{sup 18}F-labeled RGD peptide: initial evaluation for imaging brain tumor angiogenesis

Energy Technology Data Exchange (ETDEWEB)

Chen Xiaoyuan; Park, Ryan; Shahinian, Anthony H.; Tohme, Michel; Khankaldyyan, Vazgen; Bozorgzadeh, Mohammed H.; Bading, James R.; Moats, Rex; Laug, Walter E.; Conti, Peter S. E-mail: pconti@usc.edu

2004-02-01

Brain tumors are highly angiogenesis dependent. The cell adhesion receptor integrin {alpha}{sub v}{beta}{sub 3} is overexpressed in glioma and activated endothelial cells and plays an important role in brain tumor growth, spread and angiogenesis. Suitably labeled {alpha}{sub v}{beta}{sub 3}-integrin antagonists may therefore be useful for imaging brain tumor associated angiogenesis. Cyclic RGD peptide c(RGDyK) was labeled with {sup 18}F via N-succinimidyl-4-[{sup 18}F]fluorobenzoate through the side-chain {epsilon}-amino group of the lysine residue. The radiotracer was evaluated in vivo for its tumor targeting efficacy and pharmacokinetics in subcutaneously implanted U87MG and orthotopically implanted U251T glioblastoma nude mouse models by means of microPET, quantitative autoradiography and direct tissue sampling. The N-4-[{sup 18}F]fluorobenzoyl-RGD ([{sup 18}F]FB-RGD) was produced in less than 2 h with 20-25% decay-corrected yields and specific activity of 230 GBq/{mu}mol at end of synthesis. The tracer showed very rapid blood clearance and both hepatobiliary and renal excretion. Tumor-to-muscle uptake ratio at 30 min was approximately 5 in the subcutaneous U87MG tumor model. MicroPET imaging with the orthotopic U251T brain tumor model revealed very high tumor-to-brain ratio, with virtually no uptake in the normal brain. Successful blocking of tumor uptake of [{sup 18}F]FB-RGD in the presence of excess amount of c(RGDyK) revealed receptor specific activity accumulation. Hence, N-4-[{sup 18}F]fluorobenzoyl labeled cyclic RGD peptide [{sup 18}F]FB-RGD is a potential tracer for imaging {alpha}{sub v}{beta}{sub 3}-integrin positive tumors in brain and other anatomic locations.

Radium-223-Dichloride in Castration Resistant Metastatic Prostate Cancer—Preliminary Results of the Response Evaluation Using F-18-Fluoride PET/CT

Directory of Open Access Journals (Sweden)

Kalevi Kairemo

2015-10-01

Full Text Available The purpose of this study was to evaluate the outcome after Radium-223-dichloride (223RaCl2 treatment of patients with skeletal metastases of castration resistant prostate cancer using whole-body 18F-Fluoride PET/CT. Sodium 18F-fluoride [18F]-NaF PET/CT was performed prior the treatment of 223RaCl2, after the first cycle and after the sixth cycle. The skeletal metastases were analyzed quantitatively using modified PET response evaluation PERCIST criteria. The patients were also analyzed for S-PSA. All ten patients responded in [18F]-NaF scans after 6 cycles, but interim analysis after the 1st cycle did not give additional information about the outcome. The S-PSA decrease correlated with [18F]-NaF response, only 1 patient demonstrated progressive disease, i.e., >25% increase in S-PSA values during 223RaCl2. Our results (although preliminary suggest that 18F-Fluoride PET/CT is useful in the follow-up of castration resistant prostate cancer with skeletal metastases.

Synthesis and Preliminary Evaluation of 5-[18F]fluoroleucine.

Science.gov (United States)

Chin, Bennett B; McDougald, Darryl; Weitzel, Douglas H; Hawk, Thomas; Reiman, Robert E; Zalutsky, Michael R; Vaidyanathan, Ganesan

2017-01-01

Amino acid transporters, such as LAT1, are overexpressed in aggressive prostate and breast carcinomas, directly influencing pathways of growth and proliferation. The purpose of this study was to synthesize and characterize a novel 18F labeled leucine analog, 5-[18F]fluoroleucine, as a potential imaging agent for aggressive tumors which may not be amenable to imaging by FDG PET. 5-fluoroleucine was synthesized and characterized, and its 18F-labeled analog was synthesized from a mesylate precursor. First, breast cancer cell line assays were performed to evaluate uptake of 3H- or 14C-labeled L-leucine and other essential amino acids. Both L-leucine and 5- [18F]fluoroleucine were tested for uptake and accumulation over time, and for uptake via LAT1. Biodistribution studies were performed to estimate radiation dosimetry for human studies. Small animal PET / CT studies of a breast cancer were performed to evaluate in vivo 5-[18F]fluoroleucine tumor uptake. Breast cancer cell lines showed increasing high net accumulation of L-[14C]leucine. Both L-leucine and 5-[18F]fluoroleucine showed increasing uptake over time in in vitro tumor cell assays, and uptake was also shown to occur via LAT1. The biodistribution study of 5-[18F]fluoroleucine showed rapid renal excretion, no significant in vivo metabolism, and acceptable dosimetry for use in humans. In vivo small animal PET / CT imaging of a breast cancer xenograft showed uptake of 5- [18F]fluoroleucine in the tumor, which progressively increased over time. 5-[18F]fluoroleucine is a leucine analog which may be useful in identifying tumors with high or upregulated expression of amino acid transporters, providing additional information that may not be provided by FDG PET. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Synthesis and preliminary evaluation of [18F]FEtP4A, a promising PET tracer for mapping acetylcholinesterase in vivo

International Nuclear Information System (INIS)

Zhang Mingrong; Tsuchiyama, Akio; Haradahira, Terushi; Furutsuka, Kenji; Yoshida, Yuichiro; Junko Noguchi, Takayo Kida; Irie, Toshiaki; Suzuki, Kazutoshi

2002-01-01

N-[ 18 F]Fluoroethyl-4-piperidyl acetate ([ 18 F]FEtP4A), an analog of [ 11 C]MP4A for mapping brain acetylcholineseterase (AchE) activity, was prepared by reacting 4-piperidyl acetate (P4A) with [ 18 F]fluoroethyl bromide ([ 18 F]FEtBr) using a newly developed automated system. Preliminary evaluation showed that the initial uptake of [ 18 F]FEtP4A in the mouse brain was > 8% injected dose/g tissue. The distribution pattern of [ 18 F]FEtP4A in the brain was striatum>cerebral cortex>cerebellum within 10-120 min post-injection, which reflected the distribution rank pattern of AchE activity in the brain. Moreover, chemical analysis of in vivo radioactive metabolites in the mouse brain indicated that 83% of [ 18 F]FEtP4A was hydrolyzed to N-[ 18 F]fluoroethyl-4-piperidinol ([ 18 F]FEtP4OH) after 1 min intravenous injection. From these results, [ 18 F]FEtP4A may become a promising PET tracer for mapping the AchE in vivo

Hanford Waste Vitrification Plant Quality Assurance Program description for defense high-level waste form development and qualification

International Nuclear Information System (INIS)

Hand, R.L.

1992-01-01

This document describes the quality assurance (QA) program of the Hanford Waste Vitrification Plant (HWVP) Project. The purpose of the QA program is to control project activities in such a manner as to achieve the mission of the HWVP Project in a safe and reliable manner. A major aspect of the HWVP Project QA program is the control of activities that relate to high-level waste (HLW) form development and qualification. This document describes the program and planned actions the Westinghouse Hanford Company (Westinghouse Hanford) will implement to demonstrate and ensure that the HWVP Project meets the US Department of Energy (DOE) and ASME regulations. The actions for meeting the requirements of the Waste Acceptance Preliminary Specifications (WAPS) will be implemented under the HWVP product qualification program with the objective of ensuring that the HWVP and its processes comply with the WAPS established by the federal repository

Preliminary melter performance assessment report

International Nuclear Information System (INIS)

Elliott, M.L.; Eyler, L.L.; Mahoney, L.A.; Cooper, M.F.; Whitney, L.D.; Shafer, P.J.

1994-08-01

The Melter Performance Assessment activity, a component of the Pacific Northwest Laboratory's (PNL) Vitrification Technology Development (PVTD) effort, was designed to determine the impact of noble metals on the operational life of the reference Hanford Waste Vitrification Plant (HWVP) melter. The melter performance assessment consisted of several activities, including a literature review of all work done with noble metals in glass, gradient furnace testing to study the behavior of noble metals during the melting process, research-scale and engineering-scale melter testing to evaluate effects of noble metals on melter operation, and computer modeling that used the experimental data to predict effects of noble metals on the full-scale melter. Feed used in these tests simulated neutralized current acid waste (NCAW) feed. This report summarizes the results of the melter performance assessment and predicts the lifetime of the HWVP melter. It should be noted that this work was conducted before the recent Tri-Party Agreement changes, so the reference melter referred to here is the Defense Waste Processing Facility (DWPF) melter design

Active sites of the cytochrome p450cam (CYP101) F87W and F87A mutants. Evidence for significant structural reorganization without alteration of catalytic regiospecificity.

Science.gov (United States)

Tuck, S F; Graham-Lorence, S; Peterson, J A; Ortiz de Montellano, P R

1993-01-05

Ferricyanide oxidation of the aryl-iron complexes formed by the reaction of cytochrome P450 enzymes with arylhydrazines causes in situ migration of the aryl group from the iron to the porphyrin nitrogen atoms. The regiochemistry of this migration, defined by the ratio of the four possible N-arylprotoporphyrin IX isomers, provides a method for mapping the topologies of cytochrome P450 active sites. The method has been validated by using it to examine the active site of cytochrome P450cam (CYP101), for which a crystal structure is available. In agreement with the crystal structure, reaction with phenylhydrazine gives a 5:25:70 ratio of the NA:NC:ND (subscript indicates pyrrole ring) N-phenylprotoporphyrin IX isomers. Naphthylhydrazine, however, yields exclusively the NC regioisomer and 4-(phenyl)phenylhydrazine the NA:NC:ND isomers in a 14:40:46 ratio. These isomer ratio differences are readily explained by topological differences between the upper and lower reaches of the active site. Having validated the aryl-iron shift as a topological probe, we used it to investigate the structural changes caused by mutation of Phe-87, a residue that provides the ceiling over pyrrole ring D in the crystal structure of cytochrome P450cam. Mutation of Phe-87 to a tryptophan causes no detectable change in the regiochemistry of camphor hydroxylation and only minor changes in the N-aryl isomer ratios. However, mutation of Phe-87 to an alanine, which was expected to open up the region above pyrrole ring D, severely decreased the proportion of the ND in favor of the NA isomer. Less rather than more space is therefore available over pyrrole ring D in the F87A mutant despite the fact that the regiochemistry of camphor hydroxylation remains unchanged. These results provide evidence for significant structural reorganization in the upper regions of the substrate binding site without alteration of the camphor hydroxylation regiospecificity in the F87A mutant.

Skew cyclic codes over F_q+uF_q+vF_q+uvF_q

Directory of Open Access Journals (Sweden)

Ting Yao

2015-09-01

Full Text Available In this paper, we study skew cyclic codes over the ring $R=F_q+uF_q+vF_q+uvF_q$, where $u^{2}=u,v^{2}=v,uv=vu$, $q=p^{m}$ and $p$ is an odd prime. We investigate the structural properties of skew cyclic codes over $R$ through a decomposition theorem. Furthermore, we give a formula for the number of skew cyclic codes of length $n$ over $R.$

26 CFR 5c.168(f)(8)-7 - Reporting of income, deductions and investment tax credit; at risk rules.

Science.gov (United States)

2010-04-01

... tax credit; at risk rules. 5c.168(f)(8)-7 Section 5c.168(f)(8)-7 Internal Revenue INTERNAL REVENUE... investment tax credit; at risk rules. (a) In general. The fact that the lessor's payments of interest and... property shall be limited to the extent the at risk rules under the investment tax credit provisions and...

Evaluation of the 235U fission cross-section from 100 eV to 20 MeV

International Nuclear Information System (INIS)

Bhat, M.R.

1976-01-01

The evaluation of the 235 U fission cross section from 100 eV to 20 MeV for ENDF/B-V is described. The evaluated average cross sections from 100 eV to 200 keV are given, and it is proposed to include structure in the cross section in this energy region. Above 200 keV, the cross section is given as a smooth curve, and is recommended as a standard. Preliminary error estimates in the cross section are also given

Elemental abundance analyses with DAO spectrograms: XXXII. HR 6455 (A3 III), δ Aqr (A3 V), η Lep (F2 V), and 1 Boo (A1 V)

Science.gov (United States)

Yüce, K.; Adelman, S. J.; Gulliver, A. F.; Hill, G.

2011-08-01

We examine the sharp-lined stars HR 6455 (A3 III, v sin i = 8.7 km s-1) and η Lep (F2 V, v sin i = 13.5 km s-1) as well as δ Aqr (A3 V, v sin i = 81 km s-1) and 1 Boo (A1 V, v sin i = 59 km s-1) to increase the number consistently analyzed A and F stars using high dispersion and high S/N (≥200) spectrograms obtained with CCD detectors at the long Coudé camera of the 1.22-m telescope of the Dominion Astrophysical Observatory. Such studies contribute to understanding systematic abundance differences between normal and non-magnetic main-sequence band chemically peculiar A and early F stars. LTE fine analyses of HR 6455, δ Aqr, and 1 Boo using Kurucz's ATLAS suite programs show the same general elemental abundance trends with differences in the metal richness. Light and iron-peak element abundances are generally solar or overabundant while heavy element and rare earth element abundances are overabundant. HR 6455 is an evolved Am star while δ Aqr and 1 Boo show the phenomenon to different extents. Most derived abundances of η Lep are solar. Table 3 is available at the CDS via http://cdsarc.u-strasbg.fr/cgi-bin/qcat?J/AN/332/681

On a possible origin of the 2.87 eV optical transition in GaNP

International Nuclear Information System (INIS)

Buyanova, I A; Izadifard, M; Chen, W M; Xin, H P; Tu, C W

2006-01-01

Temperature dependent photoluminescence excitation spectroscopy is employed to evaluate basic physical properties of the 2.87 eV absorption peak, recently discovered for the GaN x P 1-x alloys. Whereas the appearance of this transition is found to be facilitated by incorporation of N and also H atoms, its intensity does not scale with the N content in the alloys. This questions the possible association of this feature with an N-related localized state. On the basis of the results of temperature dependent measurements, it is concluded that the state involved has a non-Γ character. Excitation of the known N-related localized states via this state is found to be non-selective, unlike that between the N-related centres. The observed properties are shown to be barely consistent with those predicted for the higher lying localized state of the isolated N atom derived from the Γ conduction band minimum (CBM). Alternative explanations for the '2.87 eV' state as being due to either a t 2 component of the X 3 c (or L 1 c ) CBM or a level arising from a complex of N and H (in some form) are also discussed

Hanford Waste Vitrification Program process development: Melt testing subtask, pilot-scale ceramic melter experiment, run summary

International Nuclear Information System (INIS)

Nakaoka, R.K.; Bates, S.O.; Elmore, M.R.; Goles, R.W.; Perez, J.M.; Scott, P.A.; Westsik, J.H.

1996-03-01

Hanford Waste Vitrification Program (HWVP) activities for FY 1985 have included engineering and pilot-scale melter experiments HWVP-11/HBCM-85-1 and HWVP-12/PSCM-22. Major objectives designated by HWVP fo these tests were to evaluate the processing characteristics of the current HWVP melter feed during actual melter operation and establish the product quality of HW-39 borosilicate glass. The current melter feed, defined during FY 85, consists of reference feed (HWVP-RF) and glass-forming chemicals added as frit

Preliminary results of the round-robin testing of F82H

Energy Technology Data Exchange (ETDEWEB)

Shiba, K.; Yamanouchi, N.; Tohyama, A.

1996-10-01

Preliminary results of metallurgical, physical and mechanical properties of low activation ferritic steel F82H (IEA heat) were obtained in the round-robin test in Japan. The properties of IEA heat F82H were almost the same as the original F82H.

Constacyclic codes over the ring F_q+v{F}_q+v2F_q and their applications of constructing new non-binary quantum codes

Science.gov (United States)

Ma, Fanghui; Gao, Jian; Fu, Fang-Wei

2018-06-01

Let R={F}_q+v{F}_q+v2{F}_q be a finite non-chain ring, where q is an odd prime power and v^3=v. In this paper, we propose two methods of constructing quantum codes from (α +β v+γ v2)-constacyclic codes over R. The first one is obtained via the Gray map and the Calderbank-Shor-Steane construction from Euclidean dual-containing (α +β v+γ v2)-constacyclic codes over R. The second one is obtained via the Gray map and the Hermitian construction from Hermitian dual-containing (α +β v+γ v2)-constacyclic codes over R. As an application, some new non-binary quantum codes are obtained.

13.-20. VI võtab ehtekunstnike rühmitus F.F.F.F....

Index Scriptorium Estoniae

2004-01-01

Rühmitus F.F.F.F. (Ketli Tiitsar, Kristi Paap, Kaire Rannik, Berit Teeäär, Maria Valdma) võtab osa Bostonis toimuvast rahvusvahelisest ehtekunstinäitusest ja konverentsist "ContacT 2004", juhendab Massachusetts College of Art üliõpilastele ja kunstnikele korraldatud workshop'i "SWOP". Bakalar Gallerys esineb rühmitus näitusega "Igapäevaleib", osalejate individuaalset loomingut eksponeerib galerii Mobilia

Comparison of mouse, guinea pig and rabbit models for evaluation of plague subunit vaccine F1+rV270.

Science.gov (United States)

Qi, Zhizhen; Zhou, Lei; Zhang, Qingwen; Ren, Lingling; Dai, Ruixia; Wu, Benchuan; Wang, Tang; Zhu, Ziwen; Yang, Yonghai; Cui, Baizhong; Wang, Zuyun; Wang, Hu; Qiu, Yefeng; Guo, Zhaobiao; Yang, Ruifu; Wang, Xiaoyi

2010-02-10

In this study, a new subunit vaccine that comprised native F1 and recombinant rV270 was evaluated for protective efficacy using mouse, guinea pig and rabbit models in comparison with the live attenuated vaccine EV76. Complete protection against challenging with 10(6) colony-forming units (CFU) of virulent Yersinia pestis strain 141 was observed for mice immunized with the subunit vaccines and EV76 vaccine. In contrast, the subunit vaccine recipes VII (F1-20 microg+rV270-10 microg) and IX (F1-40 microg+rV270-20 microg) and EV76 vaccine provided 86%, 79% and 93% protection against the same level of challenge in guinea pigs and 100%, 83% and 100% protection in rabbits, respectively. The immunized mice with the vaccines had significantly higher IgG titres than the guinea pigs and rabbits, and the immunized guinea pigs developed significantly higher IgG titres than the rabbits, but the anti-F1 response in guinea pigs was more variable than in the mice and rabbits, indicating that guinea pig is not an ideal model for evaluating protective efficacy of plague subunit vaccine, instead the rabbits could be used as an alternative model. All the immunized animals with EV76 developed a negligible IgG titre to rV270 antigen. Furthermore, analysis of IgG subclasses in the immunized animals showed a strong response for IgG1, whereas those receiving EV76 immunization demonstrated predominant production of IgG1 and IgG2a isotypes. The subunit vaccine and EV76 vaccine are able to provide protection for animals against Y. pestis challenge, but the subunit vaccines have obvious advantages over EV76 in terms of safety of use. Copyright (c) 2009 Elsevier Ltd. All rights reserved.

An Experiment to Locate the Site of TeV Flaring in M87

Energy Technology Data Exchange (ETDEWEB)

Harris, D.E.; /Harvard-Smithsonian Ctr. Astrophys.; Massaro, F.; /Harvard-Smithsonian Ctr. Astrophys. /KIPAC, Menlo Park /SLAC; Cheung, C.C.; /Natl. Acad. Sci. /Naval Research Lab, Wash., D.C.; Horns, D.; Raue, M.; /Hamburg U.; Stawarz, L.; /JAXA, Sagamihara /Jagiellonian U., Astron. Observ.; Wagner, S.; /Heidelberg Observ.; Colin, P.; /Munich, Max Planck Inst.; Mazin, D.; /Barcelona, IFAE; Wagner, R.; /Munich, Max Planck Inst.; Beilicke, M.; /McDonnell Ctr. Space Sci.; LeBohec, S.; Hui, M.; /Utah U.; Mukherjee, R.; /Barnard Coll.

2012-05-18

We describe a Chandra X-ray target-of-opportunity project designed to isolate the site of TeV flaring in the radio galaxy M87. To date, we have triggered the Chandra observations only once (2010 April) and by the time of the first of our nine observations, the TeV flare had ended. However, we found that the X-ray intensity of the unresolved nucleus was at an elevated level for our first observation. Of the more than 60 Chandra observations we have made of the M87 jet covering nine years, the nucleus was measured at a comparably high level only three times. Two of these occasions can be associated with TeV flaring, and at the time of the third event, there were no TeV monitoring activities. From the rapidity of the intensity drop of the nucleus, we infer that the size of the emitting region is of order a few light days x the unknown beaming factor; comparable to the same sort of estimate for the TeV emitting region. We also find evidence of spectral evolution in the X-ray band which seems consistent with radiative losses affecting the non-thermal population of the emitting electrons within the unresolved nucleus.

Preliminary evaluation of 1′-[18F]fluoroethyl-β-D-lactose ([18F]FEL) for detection of pancreatic cancer in nude mouse orthotopic xenografts

International Nuclear Information System (INIS)

Arumugam, Thiruvengadam; Paolillo, Vincenzo; Young, Daniel; Wen, XiaoXia; Logsdon, Craig D.; De Palatis, Louis; Alauddin, Mian M.

2014-01-01

Introduction: Early detection of pancreatic cancer could save many thousands of lives. Non-invasive diagnostic imaging, including PET with [ 18 F]FDG, has inadequate resolution for detection of small (2–3 mm) pancreatic tumours. We demonstrated the efficacy of PET imaging with an 18 F-labelled lactose derivative, [ 18 F]FEDL, that targets HIP/PAP, a biomarker that is overexpressed in the peritumoural pancreas. We developed another analogue, 1-[ 18 F]fluoroethyl lactose ([ 18 F]FEL), which is simpler to synthesise, for the same application. We conducted a preliminary evaluation of the new probe and its efficacy in detecting orthotopic pancreatic carcinoma xenografts in mice. Methods: Xenografts were developed in nude mice by injecting L3.6pl/GL + pancreatic carcinoma cells into the pancreas of each mouse. Tumour growth was monitored by bioluminescence imaging (BLI); accuracy of BLI tumour size estimates was verified by MRI in two representative mice. When the tumour size reached approximately 2–3 mm, the animals were injected with [ 18 F]FEL (3.7 MBq) and underwent static PET/CT scans. Blood samples were collected at 2, 5, 10, 20 and 60 min after [ 18 F]FEL injection to track blood clearance. Following imaging, animals were sacrificed and their organs and tumours/pancreatic tissue were collected and counted on a gamma counter. Pancreas, including tumour, was frozen, sliced and used for autoradiography and immunohistochemical analysis of HIP/PAP expression. Results: Tumour growth was rapid, as observed by BLI and MRI. Blood clearance of [ 18 F]FEL was bi-exponential, with half-lives of approximately 3.5 min and 40 min. Mean accumulation of [ 18 F]FEL in the peritumoural pancreatic tissue was 1.29 ± 0.295 %ID/g, and that in the normal pancreas of control animals was 0.090 ± 0.101 %ID/g. [ 18 F]FEL was cleared predominantly by the kidneys. Comparative analysis of autoradiographic images and immunostaining results demonstrated a correlation between [ 18 F

Evaluation of HWVP feed preparation chemistry for an NCAW simulant -- Fiscal year 1993: Effect of noble metals concentration on offgas generation and ammonia formation

International Nuclear Information System (INIS)

Patello, G.K.; Wiemers, K.D.; Bell, R.D.; Smith, H.D.; Williford, R.E.; Clemmer, R.G.

1995-03-01

The High-Level Waste Vitrification Program is developing technology for the Department of Energy to immobilize high-level and transuranic wastes as glass for permanent disposal. Pacific Northwest Laboratory (PNL) is conducting laboratory-scale melter feed preparation studies using a HWVP simulated waste slurry, Neutralized Current Acid Waste (NCAW). A FY 1993 laboratory-scale study focused on the effects of noble metals (Pd, Rh, and Ru) on feed preparation offgas generation and NH 3 production. The noble metals catalyze H 2 and NH 3 production, which leads to safety concerns. The information gained from this study is intended to be used for technology development in pilot scale testing and design of the Hanford High-Level Waste Vitrification Facility. Six laboratory-scale feed preparation tests were performed as part of the FY 1993 testing activities using nonradioactive NCAW simulant. Tests were performed with 10%, 25%, 50% of nominal noble metals content. Also tested were 25% of the nominal Rh and a repeat of 25% nominal noble metals. The results of the test activities are described. 6 refs., 28 figs., 12 tabs

Evaluation of HWVP feed preparation chemistry for an NCAW simulant -- Fiscal year 1993: Effect of noble metals concentration on offgas generation and ammonia formation

Energy Technology Data Exchange (ETDEWEB)

Patello, G.K.; Wiemers, K.D.; Bell, R.D.; Smith, H.D.; Williford, R.E.; Clemmer, R.G.

1995-03-01

The High-Level Waste Vitrification Program is developing technology for the Department of Energy to immobilize high-level and transuranic wastes as glass for permanent disposal. Pacific Northwest Laboratory (PNL) is conducting laboratory-scale melter feed preparation studies using a HWVP simulated waste slurry, Neutralized Current Acid Waste (NCAW). A FY 1993 laboratory-scale study focused on the effects of noble metals (Pd, Rh, and Ru) on feed preparation offgas generation and NH{sub 3} production. The noble metals catalyze H{sub 2} and NH{sub 3} production, which leads to safety concerns. The information gained from this study is intended to be used for technology development in pilot scale testing and design of the Hanford High-Level Waste Vitrification Facility. Six laboratory-scale feed preparation tests were performed as part of the FY 1993 testing activities using nonradioactive NCAW simulant. Tests were performed with 10%, 25%, 50% of nominal noble metals content. Also tested were 25% of the nominal Rh and a repeat of 25% nominal noble metals. The results of the test activities are described. 6 refs., 28 figs., 12 tabs.

Biological control of strawberry Fusarium wilt caused by Fusarium oxysporum f. sp. fragariae using Bacillus velezensis BS87 and RK1 formulation.

Science.gov (United States)

Nam, Myeong Hyeon; Park, Myung Soo; Kim, Hong Gi; Yoo, Sung Joon

2009-05-01

Two isolates, Bacillus sp. BS87 and RK1, selected from soil in strawberry fields in Korea, showed high levels of antagonism towards Fusarium oxysporum f. sp. fragariae in vitro. The isolates were identified as B. velezensis based on the homology of their gyrA sequences to reference strains. BS87 and RK1 were evaluated for control of Fusarium wilt in strawberries in pot trials and field trials conducted in Nonsan, Korea. In the pot trials, the optimum applied concentration of BS87 and RK1 for pre-plant root-dip application to control Fusarium wilt was 10(5) and 10(6) colony-forming units (CFU)/ml, respectively. Meanwhile, in the 2003 and 2005 field trials, the biological control efficacies of formulations of RK1 were similar to that of a conventional fungicide (copper hydroxide) when compared with a non-treated control. The RK1 formulation was also more effective than BS87 in suppressing Fusarium wilt under field conditions. Therefore, the results indicated that formulation of B.velezensis BS87 and RK1 may have potential to control Fusarium wilt in strawberries.

V and V Efforts of Auroral Precipitation Models: Preliminary Results

Science.gov (United States)

Zheng, Yihua; Kuznetsova, Masha; Rastaetter, Lutz; Hesse, Michael

2011-01-01

Auroral precipitation models have been valuable both in terms of space weather applications and space science research. Yet very limited testing has been performed regarding model performance. A variety of auroral models are available, including empirical models that are parameterized by geomagnetic indices or upstream solar wind conditions, now casting models that are based on satellite observations, or those derived from physics-based, coupled global models. In this presentation, we will show our preliminary results regarding V&V efforts of some of the models.

Hadronic origin of the TeV flare of M87 in April 2010

Energy Technology Data Exchange (ETDEWEB)

Sahu, Sarira [Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Nucleares, Circuito Exterior, C.U., A., Postal 70-543, Mexico, DF (Mexico); Palacios, Eddie [Universidad Veracruzana, Facultad de Fisica e Inteligencia Artificial, Xalapa, Veracruz (Mexico)

2015-02-01

M87 is a giant radio galaxy with FR-I morphology. It underwent three episodes of TeV flaring in recent years with the strongest one in April 2010 which was jointly monitored by MAGIC, VERITAS and H.E.S.S. We explain its spectral energy distribution in the energy range 0.3-5 TeV by assuming that the flaring occurs in the innermost region of the jet. In this region the low energy SSC photons serve as the target for the Fermi-accelerated high energy protons of energy ≤30 TeV to form a delta resonance. The TeV photons are produced from the subsequent decay of the delta resonance to neutral pions. In this scenario the observed TeV flux of April 2010 flare is fitted very well. (orig.)

Hadronic origin of the TeV flare of M87 in April 2010

International Nuclear Information System (INIS)

Sahu, Sarira; Palacios, Eddie

2015-01-01

M87 is a giant radio galaxy with FR-I morphology. It underwent three episodes of TeV flaring in recent years with the strongest one in April 2010 which was jointly monitored by MAGIC, VERITAS and H.E.S.S. We explain its spectral energy distribution in the energy range 0.3-5 TeV by assuming that the flaring occurs in the innermost region of the jet. In this region the low energy SSC photons serve as the target for the Fermi-accelerated high energy protons of energy ≤30 TeV to form a delta resonance. The TeV photons are produced from the subsequent decay of the delta resonance to neutral pions. In this scenario the observed TeV flux of April 2010 flare is fitted very well. (orig.)

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of universal stress protein F (YnaF) from Salmonella typhimurium

Energy Technology Data Exchange (ETDEWEB)

Sagurthi, Someswar Rao; Panigrahi, Rashmi Rekha; Gowda, Giri [Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012 (India); Savithri, H. S. [Department of Biochemistry, Indian Institute of Science, Bangalore 560012 (India); Murthy, M. R. N., E-mail: mrn@mbu.iisc.ernet.in [Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012 (India)

2007-11-01

The cloning, purification and crystallization of YnaF from S. typhimurium are reported along with preliminary X-ray crystallographic studies. The universal stress protein UspF (YnaF) is a small cytoplasmic bacterial protein. The expression of stress proteins is enhanced when cells are exposed to heat shock, nutrition starvation and certain other stress-inducing agents. YnaF promotes cell survival during prolonged exposure to stress and may activate a general mechanism for stress endurance. This manuscript reports preliminary crystallographic studies on YnaF from Salmonella typhimurium. The gene coding for YnaF was cloned and overexpressed and the protein was purified by Ni–NTA affinity chromatography. Purified YnaF was crystallized using vapour-diffusion and microbatch methods. The crystals belong to space group P2{sub 1}, with unit-cell parameters a = 37.51, b = 77.18, c = 56.34 Å, β = 101.8°. A data set was collected to 2.5 Å resolution with 94.6% completeness using an image-plate detector system mounted on a rotating-anode X-ray generator. Attempts to determine the structure are in progress.

Modifying the rheological properties of melter feed for the Hanford Waste Vitrification Plant

International Nuclear Information System (INIS)

Blair, H.T.; McMakin, A.H.

1986-03-01

Selected high-level nuclear wastes from the Hanford Site may be vitrified in the future Hanford Waste Vitrification Plant (HWVP) by Rockwell Hanford Company, the contractor responsible for reprocessing and waste management at the Hanford Site. The Pacific Northwest Laboratory (PNL), is responsible for providing technical support for the HWVP. In this capacity, PNL performed rheological evaluations of simulated HWVP feed in order to determine which processing factors could be modified to best optimize the vitrification process. To accomplish this goal, a simulated HWVP feed was first created and characterized. Researchers then evaluated how the chemical and physical form of the glass-forming additives affected the rheological properties and melting behavior of melter feed prepared with the simulated HWVP feed. The effects of adding formic acid to the waste were also evaluated. Finally, the maximum melter feed concentration with acceptable rheological properties was determined

Synthesis and preliminary evaluation of 9-(4-[{sup 18}F]fluoro-3-hydroxymethylbutyl) guanine ([{sup 18}F]FHBG) in HSV1-tk gene transduced hepatoma cell

Energy Technology Data Exchange (ETDEWEB)

Moon, Byung Seok; Lee, Tae Sup [Korea Institute of Radiological and Medical Sciences, Seoul (Korea, Republic of); Lee, Myoung Keun [Yonsei University, Wonju (Korea, Republic of)] (and others)

2006-08-15

The HSV1-tk reporter gene system is the most widely used system because of its advantage that direct monitoring is possible without the introduction of a separate reporter gene in case of HSV1-tk suicide gene therapy. In this study, we investigate the usefulness of the reporter probe (substrate), 9-(4-[{sup 18}F]fluoro-3-hydroxymethylbutyl) guanine ([{sup 18}F]FHBG) for non-invasive reporter gene imaging using PET in HSV1-tk expressing hepatoma model. Radiolabeled FHBG was prepared in 8 steps from a commercially available triester. The labeling reaction was carried out by NCA nucleophilic substitution with K[{sup 18}F]/K2.2.2. in acetonitrile using N2-monomethoxytrityl-9-[4-(tosly)-3-monomethoxytritylmethylbutl] guanine as a precursor, followed by deprotection with 1 N HCI. Preliminary biological properties of the probe were evaluated with MCA cells and MCA-tk cells transduced with HSV1-tk reporter gene. In vitro uptake and release-out studies of [{sup 18}F]FHBG were performed, and was analyzed correlation between [{sup 18}F]FHBG uptake ratio according to increasing numeric count of MCA-tk cells and degree of gene expression. MicroPET scan image was obtained with MCA and MCA-tk tumor beating Balb/c-nude mouse model. [{sup 18}F]FHBG was purified by reverse phase semi-HPLC system and collected at around 16-18 min. Radiochemical yield was about 20-25% (corrected for decay), radiochemical purity was > 95% and specific activity was around > 55.5 GBq/ {mu} mol. Specific accumulation of [{sup 18}F]FHBG was observed in HSV1-tk gene transduced MCA-tk cells but not MCA cells, and consecutive 1 hour release-out results showed more than 86% of uptaked [{sup 18}F]FHBG was retained inside of cells. The uptake of [{sup 18}F]FHBG was showed a highly significant linear correlation (R{sup 2} = 0.995) with increasing percentage of MCA-tk numeric cell count. In microPET scan images, remarkable difference of accumulation was observed for the two type of tumors. [{sup 18}F]FHBG appears

The impact of safety analyses on the design of the Hanford Waste Vitrification Plant

International Nuclear Information System (INIS)

Koppenaal, T.J.; Yee, A.K.; Reisdorf, J.B.; Hall, B.W.

1993-04-01

Accident analyses are being performed to evaluate and document the safety of the Hanford Waste Vitrification Plant (HWVP). The safety of the HWVP is assessed by evaluating worst-case accident scenarios and determining the dose to offsite and onsite receptors. Air dispersion modeling is done with the GENII computer code. Three accidents are summarized in this paper, and their effects on the safety and the design of the HWVP are demonstrated

Preliminary study on the evaluation of Langerhans cell histiocytosis using F-18-fluoro-deoxy-glucose PET/CT

Institute of Scientific and Technical Information of China (English)

Zhou Wenlan; Wu Hubing; Han Yanjiang; Wang Shaobo; Dong Ye; Wang Quanshi

2014-01-01

Background Limited number of studies have been reported regarding the utilization of F-18-fluoro-deoxy-glucose (F-18-FDG) positron emission tomography/computed tomography (F-18-FDG PET/CT) in Langerhans cell histiocytosis (LCH).The aim of this study was to assess the role of F-18-FDG PET/CT in the diagnosis and treatment of LCH.Methods Eight newly diagnosed and seven recurrent patients with LCH received F-18-FDG PET/CT scans.The diagnosis of LCH was established by pathology,multi-modality imaging,and clinical follow-up.Results F-18-FDG PET/CT was positive in 14 patients with 13 true positives and one false positive.All 45 LCH lesions were F-18-FDG avid including six small bone lesions ＜1.0 cm in diameter.The mean maximal standardized uptake value (SUVmax) was 7.13±4.91.F-18-FDG uptake showed no significant difference between newly diagnosed lesions vs recurrent lesions (SUVmax:6.50±2.97 vs.7.93±6.60,t=-0.901,P=0.376).Among 45 LCH lesions,68.9％ (31/45) were found in bones and 31.1％ (14/45) in soft tissue.The most commonly involved bones were the pelvis and vertebrae.There was no significant difference in F-18-FDG uptake between bone lesions vs.non-bone lesions (SUVmax:6.30±2.87 vs.8.97±7.58,t=1.277,P=0.221).In two patients,changes in F-18-FDG uptake on serial PET/CT scans reflected response of lesions to treatment.Conclusions The present study suggests that F-18-FDG PET/CT may be useful for diagnosis and assessing the treatment response of LCH.Because of the small sample size,further research is warranted to confirm our findings.

Sensorless V/f Control of Permanent Magnet Synchronous Motors

OpenAIRE

Montesinos-Miracle, Daniel; Perera, P. D. Chandana; Galceran-Arellano, Samuel; Blaabjerg, Frede

2010-01-01

V/f control strategy for permanent magnet synchronous motors can be useful for HVAC applications, where not high performance is required. Permanent magnet synchronous motors have efficiency advantages over the induction motor. But open loop V/f control is not stable in the whole frequency range. As demonstrated, the V/f control strategy becomes

Human Factors engineering criteria and design for the Hanford Waste Vitrification Plant preliminary safety analysis report

International Nuclear Information System (INIS)

Wise, J.A.; Schur, A.; Stitzel, J.C.L.

1993-09-01

This report provides a rationale and systematic methodology for bringing Human Factors into the safety design and operations of the Hanford Waste Vitrification Plant (HWVP). Human Factors focuses on how people perform work with tools and machine systems in designed settings. When the design of machine systems and settings take into account the capabilities and limitations of the individuals who use them, human performance can be enhanced while protecting against susceptibility to human error. The inclusion of Human Factors in the safety design of the HWVP is an essential ingredient to safe operation of the facility. The HWVP is a new construction, nonreactor nuclear facility designed to process radioactive wastes held in underground storage tanks into glass logs for permanent disposal. Its design and mission offer new opposites for implementing Human Factors while requiring some means for ensuring that the Human Factors assessments are sound, comprehensive, and appropriately directed

Determination of partial decay widths for the 2-, 8.87 MeV level in 16O

International Nuclear Information System (INIS)

Vermeer, W.J.; Poletti, A.R.

1982-01-01

The E2/M1 mixing ratios for the decays of the 2 - , 8.87 MeV level in 16 O to the 1 - , 7.12 MeV and 3 - , 6.13 MeV levels were measured. The results were delta(2 - → 1 - )= + 2.1 +- 0.5 and delta(2 - → 3 - )= + 2.90 +- 0.20. These are compared with previous experiments and with shell-model predictions. (author)

F.S.V.B. Volleyball

CERN Multimedia

unknown

1980-01-01

22me assemblée des délégués de la Fédération Suisse de Volley Ball (F.S.V.B.), en présence entre autres de Claude Delay, représentant de la société fédérale de gymnastique et Hans Gauer, représentant de l'association suisse de gymnastique féminine. Annonce d'une démonstration d'un mini match de volley ball à Meyrin dans l'après-midi.

18F-labelled annexin V: a PET tracer for apoptosis imaging

International Nuclear Information System (INIS)

Murakami, Yoshihiro; Tatsumi, Mitsuyoshi; Ichise, Rikiya; Nishimura, Shintaro; Takamatsu, Hiroyuki; Noda, Akihiro; Taki, Junichi; Tait, Jonathan F.

2004-01-01

Annexin V can be used to detect apoptotic cells in vitro and in vivo, based on its ability to identify extracellular phosphatidylserine, which arises during apoptosis. In the present study, we examined the synthesis of fluorine-18 labelled annexin V as a positron emission tomography tracer for apoptosis imaging. The distribution of [ 18 F]annexin V and technetium-99m labelled annexin V, a well-characterised SPET tracer for apoptosis imaging, was compared. [ 18 F]annexin V was synthesised using N-succinimidyl 4-[ 18 F]fluorobenzoate as an 18 F labelling reagent. Synthesised and purified [ 18 F]annexin V was confirmed by SDS-PAGE. In an ex vivo imaging experiment, [ 18 F]annexin V was intravenously injected into rats 24 h after the induction of myocardial ischaemia, and accumulation in the left ventricle was examined. [ 18 F]annexin V accumulated in the infarct area of the left ventricle, where apoptotic cells were observed. In separate experiments, [ 18 F]annexin V or [ 99m Tc]annexin V was intravenously injected into ischaemic or normal animals, and the distribution of the tracers was compared. In ischaemic animals, accumulation of [ 18 F]annexin V and [ 99m Tc]annexin V in the infarct area was about threefold higher than in the non-infarct area. Furthermore, the ratio of accumulation in the normal heart to the blood radioactivity was not significantly different between the tracers. In normal animals, however, the uptake of [ 18 F]annexin V in the liver, spleen and kidney was much lower than that of [ 99m Tc]annexin V. The low uptake of [ 18 F]annexin V in these organs might represent an advantage over [ 99m Tc]annexin V. (orig.)

Absolute and relative cross section measurements of 237Np(n,f and 238U(n,f at the National Physical Laboratory

Directory of Open Access Journals (Sweden)

Salvador-Castiñeira Paula

2017-01-01

Full Text Available Cross section measurements in the fast energy region are being demanded as one of the key ingredients for modelling Generation-IV nuclear power plants. However, in facilities where there are no time-of-flight possibilities or it is not convenient to use them, using the 235U(n,f cross section as a benchmark would require a careful knowledge of the room scatter in the experimental area. In this paper we present measurements of two threshold reactions, 238U(n,f and 237Np(n,f, that could become a standard between their fission threshold and 2.5 MeV, if the discrepancies shown in the evaluations and in some experimental data can be solved. The preliminary results are in agreement with the present ENDF/B-VII.1 evaluation.

[{sup 18}F]Fluciclatide in the in vivo evaluation of human melanoma and renal tumors expressing α{sub v}β{sub 3} and α{sub v}β{sub 5} integrins

Energy Technology Data Exchange (ETDEWEB)

Mena, Esther; Turkbey, Baris; Choyke, Peter; Kurdziel, Karen [NCI, NIH, Molecular Imaging Program, Bethesda, MD (United States); Owenius, Rikard [GE Healthcare, Life Sciences, Uppsala (Sweden); Sherry, Richard [NCI, NIH, Surgery Branch, Bethesda, MD (United States); Bratslavsky, Gennady [NCI, NIH, Urologic Oncology Branch, Bethesda, MD (United States); Macholl, Sven; Miller, Matthew P.; Somer, Ed J. [GE Healthcare, Life Sciences, Amersham (United Kingdom); Lindenberg, Liza [NCI, NIH, Molecular Imaging Program, Bethesda, MD (United States); National Cancer Institute, Molecular Imaging Program, Bethesda, MD (United States); Adler, Stephen [Clinical Research Directorate/CMRP SAIC-Frederick Inc., NCI-Frederick, Bethesda, MD (United States); Shih, Joanna [DCTD, NCI, NIH, Biometric Research Branch, Rockville, MD (United States)

2014-10-15

[{sup 18}F]Fluciclatide is an integrin-targeted PET radiopharmaceutical. α{sub v}β{sub 3} and α{sub v}β{sub 5} are upregulated in tumor angiogenesis as well as on some tumor cell surfaces. Our aim was to use [{sup 18}F]fluciclatide (formerly known as [{sup 18}F]AH111585) for PET imaging of angiogenesis in melanoma and renal tumors and compare with tumor integrin expression. Eighteen evaluable patients with solid tumors ≥2.0 cm underwent [{sup 18}F]fluciclatide PET/CT. All patients underwent surgery and tumor tissue samples were obtained. Immunohistochemical (IHC) staining with mouse monoclonal antibodies and diaminobenzidine (DAB) was applied to snap-frozen tumor specimens, and additional IHC was done on formalin-fixed paraffin-embedded samples. DAB optical density (OD) data from digitized whole-tissue sections were compared with PET SUV{sub 80%} {sub max}, and Patlak influx rate constant (K{sub i}) data, tumor by tumor. Tumors from all 18 patients demonstrated measurable [{sup 18}F ]fluciclatide uptake. At the final dynamic time-point (55 min after injection), renal malignancies (in 11 patients) demonstrated an average SUV{sub 80%} {sub max} of 6.4 ± 2.0 (range 3.8 - 10.0), while the average SUV{sub 80%} {sub max} for metastatic melanoma lesions (in 6 patients) was 3.0 ± 2.0 (range 0.7 - 6.5). There was a statistically significant difference in [{sup 18}F ]fluciclatide uptake between chromophobe and nonchromophobe renal cell carcinoma (RCCs), with SUV{sub 80%} {sub max} of 8.2 ± 1.8 and 5.4 ± 1.4 (P = 0.020) and tumor-to-normal kidney (T/N) ratios of 1.5 ± 0.4 and 0.9 ± 0.2, respectively (P = 0.029). The highest Pearson's correlation coefficients were obtained when comparing Patlak K{sub i} and α{sub v}β{sub 5} OD when segregating the patient population between melanoma and RCC (r = 0.83 for K{sub i} vs. melanoma and r = 0.91 for K{sub i} vs. RCC). SUV{sub 80%} {sub max} showed a moderate correlation with α{sub v}β{sub 5} and α{sub v}β{sub 3

^2H(^18F,p)^19F Study at 6 MeV/u

Science.gov (United States)

Kozub, R. L.; Nesaraja, C. D.; Moazen, B. H.; Scott, J. P.; Bardayan, D. W.; Blackmon, J. C.; Gross, C. J.; Shapira, D.; Smith, M. S.; Batchelder, J. C.; Brune, C. R.; Champagne, A. E.; Sahin, L.; Cizewski, J. A.; Thomas, J. S.; Davinson, T.; Woods, P. J.; Greife, U.; Jewett, C.; Livesay, R. J.; Ma, Z.; Parker, P. D.

2003-04-01

The degree to which the (p,α) and (p,γ) reactions destroy ^18F at temperatures ˜1-4 x 10^8 K is important for understanding the synthesis of nuclei in nova explosions and for using ^18F as a monitor of nova mechanisms in gamma ray astronomy. The reactions are dominated by low-lying proton resonances near the ^18F+p threshold (E_x=6.411 MeV excitation energy in ^19Ne). To gain further information about these resonances, we have used the inverse ^18F(d,p)^19F neutron transfer reaction at the Holifield Radioactive Ion Beam Facility to selectively populate corresponding mirror states in ^19F. Proton angular distributions were measured for states in ^19F in the excitation energy range 0-9 MeV. Results and implications for the ^18F+p reactions and nuclear structure will be presented. ^1Supported by DOE. ^2ORNL is managed by UT-Battelle, LLC, for the USDOE.

Preliminary assessment of blending Hanford tank wastes

International Nuclear Information System (INIS)

Geeting, J.G.H.; Kurath, D.E.

1993-03-01

A parametric study of blending Hanford tank wastes identified possible benefits from blending wastes prior to immobilization as a high level or low level waste form. Track Radioactive Components data were used as the basis for the single-shell tank (SST) waste composition, while analytical data were used for the double-shell tank (DST) composition. Limiting components were determined using the existing feed criteria for the Hanford Waste Vitrification Plant (HWVP) and the Grout Treatment Facility (GTF). Results have shown that blending can significantly increase waste loading and that the baseline quantities of immobilized waste projected for the sludge-wash pretreatment case may have been drastically underestimated, because critical components were not considered. Alternatively, the results suggest further review of the grout feed specifications and the solubility of minor components in HWVP borosilicate glass. Future immobilized waste estimates might be decreased substantially upon a thorough review of the appropriate feed specifications

Preliminary assessment of blending Hanford tank wastes

Energy Technology Data Exchange (ETDEWEB)

Geeting, J.G.H.; Kurath, D.E.

1993-03-01

A parametric study of blending Hanford tank wastes identified possible benefits from blending wastes prior to immobilization as a high level or low level waste form. Track Radioactive Components data were used as the basis for the single-shell tank (SST) waste composition, while analytical data were used for the double-shell tank (DST) composition. Limiting components were determined using the existing feed criteria for the Hanford Waste Vitrification Plant (HWVP) and the Grout Treatment Facility (GTF). Results have shown that blending can significantly increase waste loading and that the baseline quantities of immobilized waste projected for the sludge-wash pretreatment case may have been drastically underestimated, because critical components were not considered. Alternatively, the results suggest further review of the grout feed specifications and the solubility of minor components in HWVP borosilicate glass. Future immobilized waste estimates might be decreased substantially upon a thorough review of the appropriate feed specifications.

Search for Ψ* production in K-p interactions at 2.87 GeV/c

International Nuclear Information System (INIS)

Briefel, E.; Gourevitch, S.A.; Kirsch, L.; Schmidt, P.; Chang, C.Y.; Hemingway, R.J.; Khoury, B.V.; Stottlemyer, A.R.; Yodh, G.B.; Fernow, R.C.; Glickman, S.L.; Goldberg, M.; Jacobs, S.M.; Meadows, B.T.; Moneti, G.C.; Weygand, D.P.; Tompkins, J.; Canter, J.; Katsoufis, E.; Mann, W.A.; Schneps, J.; Wolsky, G.

1977-01-01

Evidence is presented for production of Ψ* resonances, decaying into Ψπ, Ψ (1530) π, ΛK-bar, and ΣK-bar, in K - p interactions at 2.87 GeV/c. The data represent final combined results from a 30-events/μb hydrogen exposure and an 18-events/μb exposure in deuterium designed to study Ψ* production in the mass interval 1.46--2.07 GeV/c 2 . In addition to Ψ (1820) and Ψ (1940), signals have been observed at masses of 1630 MeV/c 2 and 1860 MeV/c 2 decaying into Ψ - π + and YK-bar, respectively. Reaction cross sections have been measured for all final states containing two visible signs of strangeness, and for the final states ΛK - K + and Σ 0 K - K +

The role of active-site Phe87 in modulating the organic co-solvent tolerance of cytochrome P450 BM3 monooxygenase

International Nuclear Information System (INIS)

Kuper, Jochen; Tee, Kang Lan; Wilmanns, Matthias; Roccatano, Danilo; Schwaneberg, Ulrich; Wong, Tuck Seng

2012-01-01

Active-site Phe87 of cytochrome P450 BM3 protects the haem from DMSO molecule, thereby conferring higher organic co-solvent tolerance. Understanding the effects of organic co-solvents on protein structure and function is pivotal to engineering enzymes for biotransformation in non-aqueous solvents. The effects of DMSO on the catalytic activity of cytochrome P450 BM3 have previously been investigated and the importance of Phe87 in its organic co-solvent tolerance was identified. To probe the DMSO inactivation mechanism and the functional role of Phe87 in modulating the organic co-solvent tolerance of P450 BM3, the haem domain (Thr1–Leu455) of the F87A variant was cocrystallized in the presence of 14%(v/v) and 28%(v/v) DMSO. At both DMSO concentrations the protein retained the canonical structure of the P450 haem domain without any sign of partial or global unfolding. Interestingly, a DMSO molecule was found in the active site of both structures, with its O atom pointing towards the haem iron. The orientation of the DMSO molecule indicated a dynamic coordination process that was in competition with the active-site water molecule. The ability of the DMSO molecule to coordinate the haem iron is plausibly the main reason why P450 BM3 is inactivated at elevated DMSO concentrations. The data allowed an interesting comparison with the wild-type structures reported previously. A DMSO molecule was found when the wild-type protein was placed in 28%(v/v) DMSO, in which the DMSO molecule coordinated the haem iron directly via its S atom. Intriguingly, no DMSO molecule was observed at 14%(v/v) DMSO for the wild-type structure. These results suggested that the bulky phenyl side chain of Phe87 protects the haem from being accessed by the DMSO molecule and explains the higher tolerance of the wild-type enzyme towards organic co-solvents compared with its F87A variant

Synthesis and Molecular Structure of 6-Amino-3-benzylmercapto-1,2,4-triazolo[3,4-f][1,2,4]triazin-8(7H-one

Directory of Open Access Journals (Sweden)

Gene-Hsiang Lee

2006-03-01

Full Text Available The title compound 6-amino-3-benzylmercapto-1,2,4-triazolo[3,4-f][1,2,4]-triazin-8(7H-one (4, molecular formula C11H10N6OS, was obtained by the reaction of3-amino-2-benzyl-6-hydrazino-1,2,4-triazin-5(2H-one (3 with carbon disulfide in awater/pyridine mixture. Compound 4 can also be synthesized by reacting6-amino-3(2Hmercapto-1,2,4-triazolo[3,4-f][1,2,4]triazin-8(7H-one (7 with benzylbromide in methanolic ammonia water. The compound crystallizes in the monoclinicspace group P21/c with a = 7.2926(15, b = 14.456(2, c = 11.436(2 å, β = 105.30(2°, V= 1162.9(4 å3 and Z = 4, resulting in a density Dcalc of 1.567 g/cm3. Molecules of 4 arelinked by extensive intermolecular N-H···N and N-H···O hydrogen bonding [graph set R22 (9]. The structure is further stabilized by π-π stacking interactions. 2

The Late Devonian Frasnian-Famennian (F/F) biotic crisis: Insights from δ13C carb, δ13C org and 87Sr / 86Sr isotopic systematics

Science.gov (United States)

Chen, Daizhao; Qing, Hairuo; Li, Renwei

2005-06-01

A severe biotic crisis occurred during the Late Devonian Frasnian-Famennian (F/F) transition (± 367 Myr). Here we present δ13C carb, δ13C org and 87Sr / 86Sr isotopic systematics, from identical samples of two sections across F/F boundary in South China, which directly demonstrate large and frequent climatic fluctuations (˜200 kyr) from warming to cooling during the F/F transition. These climate fluctuations are interpreted to have been induced initially by increased volcanic outgassing, and subsequent enhanced chemical weathering linked to the rapid expansion of vascular plants on land, which would have increased riverine delivery to oceans and primary bioproductivity, and subsequent burial of organic matter, thereby resulting in climate cooling. Such large and frequent climatic fluctuations, together with volcanic-induced increases in nutrient (e.g., biolimiting Fe), toxin (sulfide) and anoxic water supply, and subsequent enhanced riverine fluxes and microbial bloom, were likely responsible for the stepwise faunal demise of F/F biotic crisis.

Significance of combined detection of JAK2V617F, MPL and CALR gene mutations in patients with essential thrombocythemia.

Science.gov (United States)

Ji, Liying; Qian, Mengyao; Wu, Nana; Wu, Jianmin

2017-03-01

The aim of this study was to analyze the mutation rate of JAK2V617F, MPLW515L/K and CALR genes in adult patients with essential thrombocythemia (ET) and the accuracy of the combined detection by the receiver operating curve. Three hundred and forty-two cases with high-platelets (≥300×10 9 /l) were consecutively selected. The patients were analyzed for routine blood examination, bone marrow biopsy and genetic testing. One hundred and fifty-four cases (45.03%) were diagnosed with ET and 188 cases of secondary thrombocythemia according to the hematopoietic and lymphoid tissue tumor classification standards of 2008. It was found that the mutant type of three genes showed three bands, whereas only one band for wild-type. The JAK2V617F and MPL mutations did not cause a change in the open reading frame and the CALR mutation resulted in its change. The mutation rate of JAK2V617F and CALR in ET group was significantly higher than that in the secondary thrombocythemia group (p<0.05). The positive mutation rate of MPL was only 4.55%. JAK2V617F-positive mutation alone was used to diagnose with ET. The area under the curve (AUC) was 0.721. The sensitivity was 72.4%, the specificity was 79.5% and the cut-off value was 0.25. When CALR-positive mutation alone was used to diagnose ET, the AUC, sensitivity, specificity and cut-off value were 0.664, 68.4, 82.4 and 0.09%, respectively. JAK2V617F combined with CALR mutation were used for diagnosis of ET. The AUC was 0.862, the sensitivity was 85.9%, the specificity was 87.8%, and the cut-off values were 0.21 and 0.07. In conclusion, the positive mutation rate of JAK2V617F and CALR in ET was higher, and the sensitivity, specificity and accuracy of the diagnosis of ET were significantly improved using the detection of JAK2V617F and CALR.

Impact of JAK2V617F Mutation Burden on Disease Phenotype in Chinese Patients with JAK2V617F-positive Polycythemia Vera (PV) and Essential thrombocythemia (ET).

Science.gov (United States)

Zhao, Shixiang; Zhang, Xiang; Xu, Yang; Feng, Yufeng; Sheng, Wenhong; Cen, Jiannong; Wu, Depei; Han, Yue

2016-01-01

Most patients with polycythemia vera (PV) and half of essential thrombocythemia (ET) possess an activating JAK2V617F mutation. The objective of this study was to better define the effect of JAK2V617F mutant allele burden on clinical phenotypes in Chinese patients, especially thrombosis. By real-time polymerase chain reaction (RT-PCR), the JAK2V617F mutation burden was detected in 170 JAK2V617F-positive patients, including 54 PV and 116 ET. The results showed that JAK2V617F allele burden was higher in PV than in ET (PET (68.5% VS 26.7%) (PET patients showed increased JAK2V617F allele burden in the group with higher hemoglobin (HGB above 150 g/L) (PET. In PV patients, JAK2V617F mutation burden had influence on WBC counts. And the clinical characteristics of ET patients, such as WBC counts, hemoglobin level, splenomegaly and thrombosis, were influenced by JAK2V617F mutation burden. Male, high hemoglobin (HGB above 150 g/L), and increased JAK2V617F mutation burden (JAK2V617F allele burden ≥ 16.5%) were risks of thrombosis (PET patients by Logistic Regression.

Assessment of realizability constraints in v2-f turbulence models

International Nuclear Information System (INIS)

Sveningsson, A.; Davidson, L.

2004-01-01

The use of the realizability constraint in v 2 -f turbulence models is assessed by computing a stator vane passage flow. In this flow the stagnation region is large and it is shown that the time scale bound suggested by [Int. J. Heat Fluid Flow 17 (1995) 89] is well suited to prevent unphysical growth of turbulence kinetic energy. However, this constraint causes numerical instabilities when used in the equation for the relaxation parameter, f. It is also shown that the standard use of the realizability constraint in the v 2 -f model is inconsistent and some modifications are suggested. These changes of the v 2 -f model are examined and shown to have negligible effect on the overall performance of the v 2 -f model. In this work two different versions of the v 2 -f model are investigated and the results obtained are compared with experimental data. The model on a form similar to that originally suggested by Durbin (e.g. [AIAA J. 33 (1995) 659]) produced the overall best agreement with stator vane heat transfer data

Application of the HWVP measurement error model and feed test algorithms to pilot scale feed testing

International Nuclear Information System (INIS)

Adams, T.L.

1996-03-01

The purpose of the feed preparation subsystem in the Hanford Waste Vitrification Plant (HWVP) is to provide, for control of the properties of the slurry that are sent to the melter. The slurry properties are adjusted so that two classes of constraints are satisfied. Processability constraints guarantee that the process conditions required by the melter can be obtained. For example, there are processability constraints associated with electrical conductivity and viscosity. Acceptability constraints guarantee that the processed glass can be safely stored in a repository. An example of an acceptability constraint is the durability of the product glass. The primary control focus for satisfying both processability and acceptability constraints is the composition of the slurry. The primary mechanism for adjusting the composition of the slurry is mixing the waste slurry with frit of known composition. Spent frit from canister decontamination is also recycled by adding it to the melter feed. A number of processes in addition to mixing are used to condition the waste slurry prior to melting, including evaporation and the addition of formic acid. These processes also have an effect on the feed composition

Production, crystallization and preliminary X-ray diffraction analysis of the allergen Can f 2 from Canis familiaris

International Nuclear Information System (INIS)

Madhurantakam, Chaithanya; Nilsson, Ola B.; Jönsson, Klas; Grönlund, Hans; Achour, Adnane

2009-01-01

The recombinant form of the allergen Can f 2 from C. familiaris was produced, isolated and crystallized in two different forms. Preliminary X-ray diffraction analyses are reported for the two crystal forms of Can f 2. The allergen Can f 2 from dog (Canis familiaris) present in saliva, dander and fur is an important cause of allergic sensitization worldwide. Here, the production, isolation, crystallization and preliminary X-ray diffraction analysis of two crystal forms of recombinant Can f 2 are reported. The first crystal form belonged to space group C222, with unit-cell parameters a = 68.7, b = 77.3, c = 65.1 Å, and diffracted to 1.55 Å resolution, while the second crystal form belonged to space group C2, with unit-cell parameters a = 75.7, b = 48.3, c = 68.7 Å, β = 126.5°, and diffracted to 2.1 Å resolution. Preliminary data analysis indicated the presence of a single molecule in the asymmetric unit for both crystal forms

Materials selection for process equipment in the Hanford waste vitrification plant

Energy Technology Data Exchange (ETDEWEB)

Elmore, M R; Jensen, G A

1991-07-01

The Hanford Waste Vitrification Plant (HWVP) is being designed to vitrify defense liquid high-level wastes and transuranic wastes stored at Hanford. The HWVP Functional Design Criteria (FDC) requires that materials used for fabrication of remote process equipment and piping in the facility be compatible with the expected waste stream compositions and process conditions. To satisfy FDC requirements, corrosion-resistant materials have been evaluated under simulated HWVP-specific conditions and recommendations have been made for HWVP applications. The materials recommendations provide to the project architect/engineer the best available corrosion rate information for the materials under the expected HWVP process conditions. Existing data and sound engineering judgement must be used and a solid technical basis must be developed to define an approach to selecting suitable construction materials for the HWVP. This report contains the strategy, approach, criteria, and technical basis developed for selecting materials of construction. Based on materials testing specific to HWVP and on related outside testing, this report recommends for constructing specific process equipment and identifies future testing needs to complete verification of the performance of the selected materials. 30 refs., 7 figs., 11 tabs.

Measurement of the 19F(n,2n)18F cross section from 18 to 27 MeV

International Nuclear Information System (INIS)

Hartmann, C.L.; DeLuca, P.M. Jr.

1990-01-01

the 19 F(n,2n) 18 F cross section was measured at neutron energies of 18, 21, 23, and 27 MeV. Nearly monoenergetic neutrons bombarded teflon (CF 2 ), Zr, and Au samples. 19 F(n,2n) 18 F cross section values were determined relative to nat Zr(n,xn) 89 Zr and 197 Au(n,2n) 196 Au from measurements of the 18 F, 89 Zr, and 196 Au activities. Our results are in agreement with previous measurements below 20 MeV and extend the usefulness of this reaction to 27 MeV. 22 refs., 1 fig., 2 tabs

Prognostic significance of ASXL1, JAK2V617F mutations and JAK2V617F allele burden in Philadelphia-negative myeloproliferative neoplasms

Directory of Open Access Journals (Sweden)

Yonal-Hindilerden I

2015-06-01

Full Text Available Ipek Yonal-Hindilerden, Aynur Daglar-Aday, Basak Akadam-Teker, Ceylan Yilmaz, Meliha Nalcaci, Akif Selim Yavuz, Deniz SarginDivision of Hematology, Department of Internal Medicine, Istanbul Medical Faculty, Istanbul University, Fatih-Istanbul, Turkey Background: Despite insights into the genetic basis of Philadelphia-negative myeloproliferative neoplasms (Ph-negative MPNs, a significant proportion of essential thrombocythemia (ET and primary myelofibrosis (PMF patients present with no known MPN disease alleles. There were no previous studies investigating the impact of ASXL1 mutations in Ph-negative MPNs in Turkey. In the current study, we investigated the prognostic significance of ASXL1 mutations in Turkish MPN patients. We also aimed to determine the prognostic significance of JAK2V617F allele burden and the relationship of JAK2V617F mutation with ASXL1 mutations in Ph-negative MPNs. Methods: About 184 patients from a single center diagnosed with Ph-negative MPNs were screened for ASXL1, JAK2V617F mutations, and JAK2V617F allele burden: 107 ET and 77 PMF. Results: A total of 29 ASXL1 mutations were detected in 24.7% of PMF and 8.4% of ET patients. ASXL1-mutated ET patients showed a trend toward an increase in the incidence of cerebrovascular events and higher total leukocyte counts. ASXL1-mutation in PMF was associated with older age and a higher prevalence of bleeding complications. In univariate analysis, overall survival (OS was significantly reduced in ASXL1-mutated PMF patients. In multivariate analysis, Dynamic International Prognostic Scoring System-plus high-risk category and ASXL1 mutation status were independently associated with shorter survival in PMF. In PMF, mutational status and allele burden of JAK2V617F showed no difference in terms of OS and leukemia-free survival. Conclusion: We conclude that ASXL1 mutations are molecular predictors of short OS in PMF. Keywords: Philadelphia-negative myeloproliferative neoplasms (Ph

242Pu: Preliminary evaluation with consideration of 240Pu, and some sensitivity results

International Nuclear Information System (INIS)

Jary, J.; Lagrange, C.; Philis, C.

1978-01-01

A preliminary evaluation of 242 Pu nuclear data is presented for the neutron energy range from 10 keV to 20 MeV. The fission cross section is based upon recent experimental measurements on 242 Pu. The remaining cross sections have been calculated using various nuclear models with parameters obtained mainly by both fits on 240 Pu experimental data and general reflexions on the actinides. Particular care has been taken of the direct interactions. The laws of secondary neutron energy spectra and the average number of neutrons produced per fission have been evaluated. The results have been placed in ENDF/BIV format and combined with the low energy region of ENDF/BIV MAT = 1161 data to make complete the evaluation over the whole energy range 10 -5 eV - 20 MeV. Finally, the sensitivities of some of these nuclear data available for reactor calculations are given in terms of the variation of the calculated critical masses

Micro-PET Imaging of αvβ3-Integrin Expression with 18F-Labeled Dimeric RGD Peptide

Directory of Open Access Journals (Sweden)

Xiaoyuan Chen

2004-04-01

Full Text Available The αv integrins, which act as cell adhesion molecules, are closely involved with tumor invasion and angiogenesis. In particular, αvβ3 integrin, which is specifically expressed on proliferating endothelial cells and tumor cells, is a logical target for development of a radiotracer method to assess angiogenesis and anti-angiogenic therapy. In this study, a dimeric cyclic RGD peptide E[c(RGDyK]2 was labeled with 18F (t1/2 = 109.7 min by using a prosthetic 4-[18F]fluorobenzoyl moiety to the amino group of the glutamate. The resulting [18F]FB-E[c(RGDyK]2, with high specific activity (200–250 GBq/μmol at the end of synthesis, was administered to subcutaneous U87MG glioblastoma xenograft models for micro-PET and autoradiographic imaging as well as direct tissue sampling to assess tumor targeting efficacy and in vivo kinetics of this PET tracer. The dimeric RGD peptide demonstrated significantly higher tumor uptake and prolonged tumor retention in comparison with a monomeric RGD peptide analog [18F]FB-c(RGDyK. The dimeric RGD peptide had predominant renal excretion, whereas the monomeric analog was excreted primarily through the biliary route. Micro-PET imaging 1 hr after injection of the dimeric RGD peptide exhibited tumor to contralateral background ratio of 9.5 ± 0.8. The synergistic effect of polyvalency and improved pharmacokinetics may be responsible for the superior imaging characteristics of [18F]FB-E[c(RGDyK]2.

Hanford Waste Vitrification Plant Technology Plan

International Nuclear Information System (INIS)

Sexton, R.A.

1988-06-01

The reference Hanford plan for disposal of defense high-level waste is based on waste immobilization in glass by the vitrification process and temporary vitrified waste storage at the Hanford Site until final disposal in a geologic repository. A companion document to the Hanford Waste Management Plan (HWMP) is the Draft, Interim Hanford Waste Management Technology Plan (HWMTP), which provides a description of the technology that must be developed to meet the reference waste management plan. One of the issues in the HWMTP is DST-6, Immobilization (Glass). The HWMTP includes all expense funding needed to complete the Hanford Waste Vitrification Plant (HWVP) project. A preliminary HWVP Technology Plan was prepared in 1985 as a supporting document to the HWMTP to provide a more detailed description of the technology needed to construct and operate a vitrification facility. The plan was updated and issued in 1986, and revised in 1987. This document is an annual update of the plan. The HWVP Technology Plan is limited in scope to technology that requires development or confirmation testing. Other expense-funded activities are not included. The relationship between the HWVP Technology Plan and other waste management issues addressed in the HWMTP is described in section 1.6 of this plan. 6 refs., 4 figs., 34 tabs

Preliminary evaluation of beta-spodumene as a fusion reactor structural material

International Nuclear Information System (INIS)

Kelsey, P.V. Jr.; Schmunk, R.E.; Henslee, S.P.

1982-01-01

Beta-spodumene was investigated as a candidate material for use in fusion reactor environments. Properties which support the use of beta-spodumene include good thermal shock resistance, a very low coefficient of thermal expansion, a low-Z composition which would result in minimum impact on the plasma, and flexibility in fabrication processes. Specimens were irradiated in the Advanced Test Reactor (ATR) to a fluence of 5.3 x 10 22 n/m 2 , E > MeV, and 4.9 x 10 23 n/m 2 thermal fluence in order to obtain a preliminary evaluation of the impact of irradiation on the material. Preliminary data indicate that the mechanical properties of beta-spodumene are little affected by irradiation. Gas production and release have also been investigated. (orig.)

Preliminary study of the 19F(7Li,7Be)19O reaction at 52 MeV with MAGNEX

CERN Document Server

Cavallaro, M; Cappuzzello, F; Carbone, D; Foti, A; Orrigo, S E A; Rodrigues, M R D; Schillaci, M; Borello-Lewin, T; Petrascu, H

2010-01-01

The 19F(7Li,7Be)19O charge-exchange reaction at 52 MeV incident energy has been performed at INFN-LNS in Catania using the MAGNEX spectrometer. The use of an algebraic ray-reconstruction technique has allowed to extract the 19O excitation energy spectrum and the experimental angular distributions obtained with a single angular setting of the spectrometer.

Simultaneous observations of electromagnetically induced transparency (EIT) and absorption (EIA) in a multi-level V-type system of 87Rb and theoretical simulation of the observed spectra using a multi-mode approach.

Science.gov (United States)

Das, Bankim Chandra; Bhattacharyya, Dipankar; Das, Arpita; Chakrabarti, Shrabana; De, Sankar

2016-12-14

We report here simultaneous experimental observation of Electromagnetically Induced Transparency (EIT) and Electromagnetically Induced Absorption (EIA) in a multi-level V-type system in D 2 transition of Rb87, i.e., F=2→F ' with a strong pump and a weak probe beam. We studied the probe spectrum by locking the probe beam to the transition F=2→F ' =2 while the pump is scanned from F=2→F ' . EIA is observed for the open transition (F=2→F ' =2) whereas EIT is observed in the closed transition (F=2→F ' =3). Sub natural line-width is observed for the EIA. To simulate the observed spectra theoretically, Liouville equation for the three-level V-type system is solved analytically with a multi-mode approach for the density matrix elements. We assumed both the pump and the probe beams can couple the excited states. A multi-mode approach for the coherence terms facilitates the study of all the frequency contributions due to the pump and the probe fields. Since the terms contain higher harmonics of the pump and the probe frequencies, we expressed them in Fourier transformed forms. To simulate the probe spectrum, we have solved inhomogeneous difference equations for the coherence terms using the Green's function technique and continued fraction theory. The experimental line-widths of the EIT and the EIA are compared with our theoretical model. Our system can be useful in optical switching applications as it can be precisely tuned to render the medium opaque and transparent simultaneously.

Measurement of the 234U(n, f ) cross-section with quasi-monoenergetic beams in the keV and MeV range using a Micromegas detector assembly

Science.gov (United States)

Stamatopoulos, A.; Kanellakopoulos, A.; Kalamara, A.; Diakaki, M.; Tsinganis, A.; Kokkoris, M.; Michalopoulou, V.; Axiotis, M.; Lagoyiannis, A.; Vlastou, R.

2018-01-01

The 234U neutron-induced fission cross-section has been measured at incident neutron energies of 452, 550, 651 keV and 7.5, 8.7, 10 MeV using the 7Li ( p, n) and the 2H( d, n) reactions, respectively, relative to the 235U( n, f ) and 238U( n, f ) reference reactions. The measurement was performed at the neutron beam facility of the National Center for Scientific Research "Demokritos", using a set-up based on Micromegas detectors. The active mass of the actinide samples and the corresponding impurities were determined via α-spectroscopy using a surface barrier silicon detector. The neutron spectra intercepted by the actinide samples have been thoroughly studied by coupling the NeuSDesc and MCNP5 codes, taking into account the energy and angular straggling of the primary ion beams in the neutron source targets in addition to contributions from competing reactions ( e.g. deuteron break-up) and neutron scattering in the surrounding materials. Auxiliary Monte Carlo simulations were performed making combined use of the FLUKA and GEF codes, focusing particularly on the determination of the fission fragment detection efficiency. The developed methodology and the final results are presented.

Brown Adipose Tissue Can Be Activated or Inhibited within an Hour before 18F-FDG Injection: A Preliminary Study with MicroPET

Directory of Open Access Journals (Sweden)

Chenxi Wu

2011-01-01

Full Text Available Brown adipose tissue (BAT is emerging as a potential target for treating human obesity. It has been indicated that BAT is rich in innervations of sympathetic nerve control. Using 18F-FDG microPET imaging, this study aims at evaluating how factors related to sympathetic activation/inhibition changed BAT metabolism of mice. BAT 18F-FDG uptake were semiquantitatively evaluated in different groups of mice under temperature (cold or warm stimulus or pharmacological interventions (norepinephrine, epinephrine, isoprenaline, or propranolol and were compared with the corresponding controls. It was found that BAT activation can be stimulated by cold exposure (P=1.96×10−4, norepinephrine (P=.002, or both (P=2.19×10−6 within an hour before 18F-FDG injection and can also be alleviated by warming up (P=.001 or propranolol lavage (P=.027. This preliminary study indicated that BAT function could be evaluated by 18F-FDG PET imaging through short-term interventions, which paved the way for further investigation of the relationship between human obesity and BAT dysfunction.

Preliminary design of GDT-based 14 MeV neutron source

International Nuclear Information System (INIS)

Du Hongfei; Chen Dehong; Wang Hui; Wang Fuqiong; Jiang Jieqiong; Wu Yican; Chen Yiping

2012-01-01

To meet the need of D-T fusion neutron source for fusion material testing, design goals were presented in this paper according to the international requirements of neutron source for fusion material testing. A preliminary design scheme of GDT-based 14 MeV neutron source was proposed, and a physics model of the neutron source was built based on progress of GDT experiments. Two preliminary design schemes (i. e. FDS-GDT1, FDS-GDT2) were designed; among which FDS-GDT2 can be used for fusion material testing with neutron first wall loading of 2 MW/m 2 . (authors)

Synthesis and evaluation of an 18F-labeled pyrimidine-pyridine amine for targeting CXCR4 receptors in gliomas

International Nuclear Information System (INIS)

Demoin, Dustin Wayne; Shindo, Masahiro; Zhang, Hanwen; Edwards, Kimberly J.; Serganova, Inna; Pillarsetty, Naga Vara Kishore; Lewis, Jason S.; Blasberg, Ronald G.

2016-01-01

Introduction: Chemokine receptor-4 (CXCR4, fusin, CD184) is expressed on several tissues involved in immune regulation and is upregulated in many diseases including malignant gliomas. A radiolabeled small molecule that readily crosses the blood–brain barrier can aid in identifying CXCR4-expressing gliomas and monitoring CXCR4-targeted therapy. In the current work, we have synthesized and evaluated an [ 18 F]-labeled small molecule based on a pyrimidine–pyridine amine for its ability to target CXCR4. Experimental: The nonradioactive standards and the nitro precursor used in this study were prepared using established methods. An HPLC method was developed to separate the nitro-precursor from the nonradioactive standard and radioactive product. The nitro-precursor was radiolabeled with 18 F under inert, anhydrous conditions using the [ 18 F]-kryptofix 2.2.2 complex to form the desired N-(4-(((6-[ 18 F]fluoropyridin-2-yl)amino)methyl)benzyl)pyrimidin-2-amine ([ 18 F]-3). The purified radiolabeled compound was used in serum stability, partition coefficient, cellular uptake, and in vivo cancer targeting studies. Results: [ 18 F]-3 was synthesized in 4–10% decay-corrected yield (to start of synthesis). [ 18 F]-3 (t R ≈ 27 min) was separated from the precursor (t R ≈ 30 min) using a pentafluorophenyl column with an isocratic solvent system. [ 18 F]-3 displayed acceptable serum stability over 2 h. The amount of [ 18 F]-3 bound to the plasma proteins was determined to be > 97%. The partition coefficient (LogD 7.4 ) is 1.4 ± 0.5. Competitive in vitro inhibition indicated 3 does not inhibit uptake of 67 Ga-pentixafor. Cell culture media incubation and ex vivo urine analysis indicate rapid metabolism of [ 18 F]-3 into hydrophilic metabolites. Thus, in vitro uptake of [ 18 F]-3 in CXCR4 overexpressing U87 cells (U87 CXCR4) and U87 WT indicated no specific binding. In vivo studies in mice bearing U87 CXCR4 and U87 WT tumors on the left and right shoulders were carried

Re(CO)3([18F]FEDA), a novel 18F PET renal tracer: Radiosynthesis and preclinical evaluation.

Science.gov (United States)

Lipowska, Malgorzata; Jarkas, Nashwa; Voll, Ronald J; Nye, Jonathon A; Klenc, Jeffrey; Goodman, Mark M; Taylor, Andrew T

2018-03-01

Our previous work demonstrated that the 99m Tc renal tracer, 99m Tc(CO) 3 (FEDA) ( 99m Tc-1), has a rapid clearance comparable in rats to that of 131 I-OIH, the radioactive gold standard for the measurement of effective renal plasma flow. The uncharged fluoroethyl pendant group of 99m Tc-1 provides a route to the synthesis of a structurally analogous rhenium-tricarbonyl 18 F renal imaging agent, Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1). Our goal was to develop an efficient one-step method for the preparation of 18 F-1 and to compare its pharmacokinetic properties with those of 131 I-OIH in rats. 18 F-1 was prepared by the nucleophilic 18 F-fluorination of its tosyl precursor. The labeled compound was isolated by HPLC and subsequently evaluated in Sprague-Dawley rats using 131 I-OIH as an internal control and by dynamic PET/CT imaging. Plasma protein binding (PPB) and erythrocyte uptake (RCB) were determined and the urine was analyzed for metabolites. 18 F-1 was efficiently prepared as a single species with high radiochemical purity (>99%) and it displayed high radiochemical stability in vitro and in vivo. PPB was 87% and RCB was 21%. Biodistribution studies confirmed rapid renal extraction and high specificity for renal excretion, comparable to that of 131 I-OIH, with minimal hepatic/gastrointestinal elimination. The activity in the urine, as a percentage of 131 I-OIH, was 92% and 95% at 10 and 60 min, respectively. All other organs (heart, spleen, lungs) showed a negligible tracer uptake (F-1 through the kidneys and into the bladder; there was no demonstrable activity in bone verifying the absence of free [ 18 F]fluoride. 18 F-1 exhibited a high specificity for the kidney, rapid renal excretion comparable to that of 131 I-OIH and high in vivo radiochemical stability. Not only is 18 F-1 a promising PET renal tracer, but it provides a route to the development of a pair of analogous 18 F/ 99m Tc renal imaging agents with almost identical structures and comparable

Assessment of a recombinant F1-V fusion protein vaccine intended to protect Canada lynx (Lynx canadensis) from plague

Science.gov (United States)

Wolfe, Lisa L.; Shenk, Tanya M.; Powell, Bradford; Rocke, Tonie E.

2011-01-01

As part of an ongoing restoration program in Colorado, USA, we evaluated adverse reactions and seroconversion in captive Canada lynx (Lynx canadensis) after vaccination with a recombinant F1-V fusion protein vaccine against Yersinia pestis, the bacterium that causes plague. Ten adult female lynx received the F1-V vaccine; 10 source- and age-matched lynx remained unvaccinated as controls. All of the vaccinated and control lynx remained apparently healthy throughout the confinement period. We observed no evidence of injection site or systemic reactions to the F1-V vaccine. Among vaccinated lynx, differences in log10 reciprocal antibody titers measured in sera collected before and after vaccination (two doses) ranged from 1.2 to 5.2 for anti-F1 antibodies and from 0.6 to 5.2 for anti-V antibodies; titers in unvaccinated lynx did not change appreciably over the course of confinement prior to release, and thus differences in anti-F1 (P=0.003) and anti-V (P=0.0005) titers were greater among vaccinated lynx than among controls. Although our findings suggest that the F1-V fusion protein vaccine evaluated here is likely to stimulate antibody responses that may help protect Canada lynx from plague, we observed no apparent differences in survival between vaccinated and unvaccinated subject animals. Retrospectively, 22 of 50 (44%; 95% confidence interval 29–59%) unvaccinated lynx captured or recaptured in Colorado during 2000–08 had passive hemagglutination antibody titers >1:16, consistent with exposure to Y. pestis; paired pre- and postrelease titers available for eight of these animals showed titer increases similar in magnitude to those seen in response to vaccination, suggesting at least some lynx may naturally acquire immunity to plague in Colorado habitats.

JAK2V617F mutation is associated with special alleles in essential thrombocythemia.

Science.gov (United States)

Hsiao, Hui-Hua; Liu, Yi-Chang; Tsai, Hui-Jen; Lee, Ching-Ping; Hsu, Jui-Feng; Lin, Sheng-Fung

2011-03-01

Janus kinase 2 mutation (JAK2V617F) has been identified in myeloproliferative neoplasms. Furthermore, special single nucleoside polymorphisms (SNPs) have been found to be associated with the JAK2V617F mutation. Therefore, the associations among JAK2V617F and special SNPs and the allelic location between them were investigated in patients with essential thrombocythemia (ET). A total of 61 patients with ET and 106 healthy individuals were enrolled. The PCR-RFLP method was applied to investigate the pattern of three SNPs, rs10974944, rs12343867, and rs12340895. Allele-specific PCR was used to examine the allelic location between rs10974944 and JAK2V617F. Among the patients with ET, 34 (55.7%, 34/61) were JAK2V617F positive (heterozygous) while the other 27 (44.3%, 27/61) were negative, and there were no MPLW515L/K mutations noted. The pattern of special SNPs in JAK2V617F(+) was significantly different from that in normal individuals (p <0.05), while there was no difference between JAK2V617F(-) patients and normal individuals. Allele-specific PCR showed high association of a cis-location between the special G-allele of rs10974944 and JAK2V617F(+). Based on this small numbered study, the results show the association between special SNPs and JAK2V617F mutation and a cis-location between the special G-allelic form of rs10974944 and the JAK2V617F mutation. These data highlight a close relationship between them in patients with ET.

Sällskapsdjurens betydelse för de äldres välbefinnande

OpenAIRE

Gäddnäs, Emma

2016-01-01

Denna studie undersöker sällskapsdjurens betydelse för de äldres välbefinnande. Syftet med studien är att diskutera vad sällskapsdjur betyder för de äldres välbefinnande och vad djur kan betyda för de äldre som bor på äldreboende. Frågeställningarna i studien lyder ”Vilken betydelse har sällskapsdjur för de äldres välbefinnande och deras känsla av delaktighet?” och ”Vilken betydelse har sällskapsdjur inom äldreomsorgen?” Metoden som används för att svara på frågeställningarna är både tidigare...

Hanford Waste Vitrification Plant Project advanced conceptual design summary report

International Nuclear Information System (INIS)

Anderson, T.D.

1988-11-01

The Hanford Waste Vitrification Plant (HWVP) will immobilize Hanford defense liquid high-level waste in borosilicate glass in preparation for shipment to a geologic repository. The shipment of the waste to the repository will satisfy an objective in the President's Defense Waste Management Plan. The glass product will be cast into stainless steel canisters, which will be sealed and stored at Hanford until they are shipped. This document summarizes work performed during the Advance Conceptual Design (ACD) of the HWVP. In the Reference Conceptual Design phase, which preceded the ACD, a number of design issues were identified with the potential to improve cost effectiveness, safety, constructibility, and operability. The ACD addressed and evaluated these design issues. Implementation of recommendations derived from ACD work will occur in subsequent design phases. The next design phase is preliminary design which will be followed by detailed design and construction. Net potential cost improvements of more than $36.9M were identified along with improvements in safety, constructibility, and operability. No negative schedule impacts will result from implementation of the improvements. 11 refs., 5 figs., 3 tabs

Preliminary design of a 150 kJ repetitive plasma focus for the production of 18-F

Science.gov (United States)

Sumini, Marco; Mostacci, Domiziano; Rocchi, Federico; Frignani, Michele; Tartari, Agostino; Angeli, Ergisto; Galaverni, Dario; Coli, Ugo; Ascione, Bernardino; Cucchi, Giorgio

2006-06-01

Experiments in the past five years have demonstrated production of short-lived radioisotopes with a Plasma Focus device, using the so-termed Endogenous Mode. So far radioisotope activities of only a few microcuries have been obtained from single discharges in small scale Plasma Focus machines (capacitor bank energies of approximately 7 kJ). It is expected that higher activities could be obtained with larger bank energies, operating at high pulse repetition rates, e.g. 1 Hz. However, many scientific and technological issues must be addressed for a high-energy Plasma Focus device to run at one pulse per second. Aim of this paper is to present preliminary results pertaining to the plasma, electrical, fluid-dynamical, thermal, material and mechanical design of a 150 kJ Plasma Focus, capable of a repetition rate of 1 Hz, that will be operated at 30 kV with a 350 μF capacitor bank and a maximum total current of 1.5 MA. This device will be used to breed 18-F for the synthesis of drugs used in positron-emission medical examinations, such as FDG for PET.

Preliminary design of a 150 kJ repetitive plasma focus for the production of 18-F

International Nuclear Information System (INIS)

Sumini, Marco; Mostacci, Domiziano; Rocchi, Federico; Frignani, Michele; Tartari, Agostino; Angeli, Ergisto; Galaverni, Dario; Coli, Ugo; Ascione, Bernardino; Cucchi, Giorgio

2006-01-01

Experiments in the past five years have demonstrated production of short-lived radioisotopes with a Plasma Focus device, using the so-termed Endogenous Mode. So far radioisotope activities of only a few microcuries have been obtained from single discharges in small scale Plasma Focus machines (capacitor bank energies of approximately 7 kJ). It is expected that higher activities could be obtained with larger bank energies, operating at high pulse repetition rates, e.g. 1 Hz. However, many scientific and technological issues must be addressed for a high-energy Plasma Focus device to run at one pulse per second. Aim of this paper is to present preliminary results pertaining to the plasma, electrical, fluid-dynamical, thermal, material and mechanical design of a 150 kJ Plasma Focus, capable of a repetition rate of 1 Hz, that will be operated at 30 kV with a 350 μF capacitor bank and a maximum total current of 1.5 MA. This device will be used to breed 18-F for the synthesis of drugs used in positron-emission medical examinations, such as FDG for PET

F-1 Engine for Saturn V Undergoing a Static Test

Science.gov (United States)

1964-01-01

The flame and exhaust from the test firing of an F-1 engine blast out from the Saturn S-IB Static Test Stand in the east test area of the Marshall Space Flight Center. A Cluster of five F-1 engines, located in the S-IC (first) stage of the Saturn V vehicle, provided over 7,500,000 pounds of thrust to launch the giant rocket. The towering 363-foot Saturn V was a multistage, multiengine launch vehicle standing taller than the Statue of Liberty. Altogether, the Saturn V engines produced as much power as 85 Hoover Dams.

Evaluation of uterine cervix cancer hypoxia by PET with {sup 18}F-F.E.T.N.I.M.. Relation with squamous cell carcinoma (S.C.C.) and osteopontin tumoral markers; Evaluation de l'hypoxie du cancer du col uterin par la TEP au {sup 18}F-FETNIM. Relation avec les marqueurs tumoraux SCC et osteopontine

Energy Technology Data Exchange (ETDEWEB)

Barre, E.; Schlageter, M.H.; Groheux, D.; Vercellino, L.; Lussato, D.; Thoury, A.; Hennequin, V.; Hindie, E.; Barranger, E.; Moretti, J.L. [CHU Saint-Louis-Lariboisiere, universite Paris-7, IMDCT, 75 - Paris (France)

2010-07-01

Purpose: to evaluate the imaging feasibility of hypoxia of cervical squamous cell carcinoma by PET with {sup 18}F-F.E.T.N.I.M. (Iason). to compare the {sup 18}F-F.E.T.N.I.M. uptake to the metabolic uptake in {sup 18}F-F.D.G. and to study their relationship with the squamous cell carcinoma, tumoral marker and osteoponin, circulating tracer of hypoxia. Conclusions: the F.E.T.N.I.M. uptake by the tumor is always of lower level than the {sup 18}F-F.D.G. uptake. By using the ratio Tumor/Muscle, these preliminary results do not enlighten any correlation between the circulating hypoxia tracer and the tumor uptake of {sup 18}F-F.E.T.N.I.M.. (N.C.)

Infection Imaging With 18F-FDS and First-in-Human Evaluation

International Nuclear Information System (INIS)

Yao, Shaobo; Xing, Haiqun; Zhu, Wenjia; Wu, Zhanhong; Zhang, Yingqiang; Ma, Yanru; Liu, Yimin; Huo, Li; Zhu, Zhaohui; Li, Zibo; Li, Fang

2016-01-01

Purpose: The noninvasive imaging of bacterial infections is critical in order to reduce mortality and morbidity caused by these diseases. The recently reported 18 F-FDS ( 18 F-2-fluorodeoxy sorbitol) as a PET (positron emission tomography) tracer can be used to image Enterobacteriaceae-specific infections and provides a potential alternative to this problem compared with other probes for imaging infections. In this study, automatic synthesis, validation of 18 F-FDS and a first-in-human study were performed and discussed. Methods: A multifunctional synthesis module was employed for the radiosynthesis of 18 F-FDG ( 18 F-2-fluorodeoxy glucose) and 18 F-FDS starting from 18 F ion using two-pot three-step fully automated reactions. The behavior of 18 F-FDS as an in vivo imaging probe for infections was evaluated in an Escherichia coli mouse infection model. The first detailed pharmacokinetic and biodistribution parameters were obtained from healthy human volunteers. Results: The uptake of 18 F-FDS in an E. coli mouse-myositis infection model was easily differentiated from other organs and normal muscle. Intensive lesion uptake declined after antibiotic treatment. In the pilot human study, no adverse effects due to 18 F-FDS were observed up to 24 h post-injection. The radiotracer was rapidly cleared from the circulation and excreted mainly through the urinary system. Conclusion: We conclude that 18 F-FDS PET holds great potential for appropriate and effective for the imaging of bacterial infections in vivo. These preliminary results indicate that further clinical studies are warranted.

Methodological development for 87Sr/86Sr measurement in olive oil and preliminary discussion of its use for geographical traceability of PDO Nîmes (France).

Science.gov (United States)

Medini, Salim; Janin, Myriam; Verdoux, Patrick; Techer, Isabelle

2015-03-15

The lack of a geographical identification protocol for olive oils can lead to fraud and health risks. As some works call for Sr isotopes for the geographical identification of agri-food products, this study focus on the feasibility of extracting Sr from olive oils for isotopic measurements by TIMS. In fact, existing protocols for purification of Sr are unsuitable for lipid matrix. The defined protocol is applied to samples of PDO Nîmes olive oil. The accuracy of the extraction procedure is tested against isotopic standards. The values obtained are in conformity with NIST certified values. This consistency demonstrates that no modification of (87)Sr/(86)Sr ratio is brought about by this protocol. Consequently, the method is preliminary used on PDO Nîmes and Moroccan oils to evaluate the feasibility of a discriminant Sr signature on the two geographical products. This study provides promising results for the geographical discrimination and identification of PDO olive oils. Copyright © 2014 Elsevier Ltd. All rights reserved.

Measurement of the {sup 234}U(n, f) cross-section with quasi-monoenergetic beams in the keV and MeV range using a Micromegas detector assembly

Energy Technology Data Exchange (ETDEWEB)

Stamatopoulos, A.; Kanellakopoulos, A.; Kalamara, A.; Kokkoris, M.; Michalopoulou, V.; Vlastou, R. [National Technical University of Athens, Department of Physics, Athens (Greece); Diakaki, M. [National Technical University of Athens, Department of Physics, Athens (Greece); European Organisation for Nuclear Research (CERN), Geneva (Switzerland); Tsinganis, A. [European Organisation for Nuclear Research (CERN), Geneva (Switzerland); Axiotis, M.; Lagoyiannis, A. [Tandem Accelerator Laboratory, Institute of Nuclear and Particle Physics, N.C.S.R. Demokritos, Athens (Greece)

2018-01-15

The {sup 234}U neutron-induced fission cross-section has been measured at incident neutron energies of 452, 550, 651 keV and 7.5, 8.7, 10 MeV using the {sup 7}Li (p, n) and the {sup 2}H(d, n) reactions, respectively, relative to the {sup 235}U(n, f) and {sup 238}U(n, f) reference reactions. The measurement was performed at the neutron beam facility of the National Center for Scientific Research ''Demokritos'', using a set-up based on Micromegas detectors. The active mass of the actinide samples and the corresponding impurities were determined via α-spectroscopy using a surface barrier silicon detector. The neutron spectra intercepted by the actinide samples have been thoroughly studied by coupling the NeuSDesc and MCNP5 codes, taking into account the energy and angular straggling of the primary ion beams in the neutron source targets in addition to contributions from competing reactions (e.g. deuteron break-up) and neutron scattering in the surrounding materials. Auxiliary Monte Carlo simulations were performed making combined use of the FLUKA and GEF codes, focusing particularly on the determination of the fission fragment detection efficiency. The developed methodology and the final results are presented. (orig.)

/sup 87/Rb NMR study at the cubic to tetragonal phase transition in RbCaF/sub 3/

Energy Technology Data Exchange (ETDEWEB)

Bulou, A [Angers Univ., 72 - Le Mans (France). Centre Universitaire; Theveneau, H; Trokiner, A; Papon, P [Ecole Superieure de Physique et Chimie Industrielles, 75 - Paris (France)

1979-07-01

The /sup 87/Rb nuclear magnetic resonance spectrum, in perovskite single crystal of RbCaF/sub 3/, is studied above and below the cubic-to-tetragonal phase transition occurring at 198 K. In the high-temperature cubic phase, the temperature dependence of the resonance line amplitude deviates from the Curie law and this can be attributed to the existence of tetragonal domains. In the low temperature tetragonal phase, a second-order quadrupole shift of the central line is observed, from which the CaF/sub 6/ tilt angle (order parameter) is derived. The order parameter temperature dependence is described by a power law with a cross over from exponent 0.5 to exponent 0.32 at 150 K. The tilt angle PHI is compared to the values obtained from X-ray and neutron powder diffraction data.

Preliminary Evaluation on Characteristics of Waste from Pyro-processing (FS v5.1)

International Nuclear Information System (INIS)

Kim, In-Young; Choi, Heui-Joo

2016-01-01

In this study, characteristic of waste from pyro-processing which based on material balance FS v5.1 is evaluated for revising of A-KRS concept. To reduce volume and toxicity of PWR SNFs, the P and T Technology using pyro-processing and SFR is under development in KAERI. In accordance with this R and D, the A-KRS was developed by KAERI for disposal of waste from pyro-processing. After A-KRS concept development, material balance has been revised and characteristics of waste have been changed. To solve impending saturation of storage capacity of NPP sites, national policy on SNF management seems to be determined shortly. Demand on detailed analysis on impact of P and T using pyro-processing and SFR as base data is increasing. To compare direct disposal scenario to the P and T scenario using pyro-processing and SFR, updating of A-KRS reflecting amendment of material balance is required. In this study, characteristic of waste from pyro-processing which based on material balance FS v5.1 is evaluated. Every 15 types of outputs generated from pyro-processing are evaluated. Great reduction can be achieved by complete reuse of U/TRU/RE in SFR, because most of decay heat, radioactivity and radiotoxicity are generated from U/TRU/RE ingot. Within about 300 years, the fly ash filter containing Cs, Sr containing salt waste are important waste in perspective of decay heat and radioactivity. Importance of RE containing salt waste reduced. Management of hold-ups must be clarified because hold-ups take large portion of decay heat, radioactivity in the material balance FS v5.1

Preliminary Evaluation on Characteristics of Waste from Pyro-processing (FS v5.1)

Energy Technology Data Exchange (ETDEWEB)

Kim, In-Young; Choi, Heui-Joo [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

2016-10-15

In this study, characteristic of waste from pyro-processing which based on material balance FS v5.1 is evaluated for revising of A-KRS concept. To reduce volume and toxicity of PWR SNFs, the P and T Technology using pyro-processing and SFR is under development in KAERI. In accordance with this R and D, the A-KRS was developed by KAERI for disposal of waste from pyro-processing. After A-KRS concept development, material balance has been revised and characteristics of waste have been changed. To solve impending saturation of storage capacity of NPP sites, national policy on SNF management seems to be determined shortly. Demand on detailed analysis on impact of P and T using pyro-processing and SFR as base data is increasing. To compare direct disposal scenario to the P and T scenario using pyro-processing and SFR, updating of A-KRS reflecting amendment of material balance is required. In this study, characteristic of waste from pyro-processing which based on material balance FS v5.1 is evaluated. Every 15 types of outputs generated from pyro-processing are evaluated. Great reduction can be achieved by complete reuse of U/TRU/RE in SFR, because most of decay heat, radioactivity and radiotoxicity are generated from U/TRU/RE ingot. Within about 300 years, the fly ash filter containing Cs, Sr containing salt waste are important waste in perspective of decay heat and radioactivity. Importance of RE containing salt waste reduced. Management of hold-ups must be clarified because hold-ups take large portion of decay heat, radioactivity in the material balance FS v5.1.

Effect of D.C. testing water tree deteriorated cable and a preliminary evaluation of V.L.F. as alternate

International Nuclear Information System (INIS)

Eager, G.S. Jr.; Fryszczyn, B.; Katz, C.; ElBadaly, H.A.; Jean, A.R.

1992-01-01

This paper reports that according to the experience of some power utilities, the application of industry recommended high voltage d.c. field tests on 5-35 kV extruded dielectric cables, containing water trees, sometimes causes further deterioration of the insulation. Tests conducted on laboratory aged 15 kV ethylene propylene rubber (EP) and 15 and 28 kV crosslinked polyethylene (XLPE) insulated cables indicate that d.c. proof tests in accordance with AEIC specifications an IEEE test guides without flashover do not appear to cause further deterioration. Depending on the degree of cable aging and the level of test voltage, when flashovers take place, damage may be inflicted to XLPE cables. No damage was observed on aged EP cable, at the same test levels. Because of the aforementioned power utility experience, some users have requested an alternate field proof test. Tests conducted on new XLPE and EP cables indicate that damage to the insulation structure can be detected using VLF (0.1 Hz) voltage at approximately one-third the d.c. voltage level. Field tests conducted on severely tree deteriorated 15 kV polyethylene (PE) cable using AEIC recommended d.c. voltage level of about five times operating voltage level caused cable failure; VLF voltage levels up to two times operating voltage did not. VLF voltage appears to be a suitable alternate to d.c. voltage for field proof testing

Evaluation of Nd-Loaded SnO2:F Films Coated via Spray Pyrolysis

Science.gov (United States)

Turgut, G.

2018-07-01

Thin layers of single (F)- and double (F/Nd)-incorporated tin oxide have been coated on glass substrate via spray pyrolysis. The structural, morphological, electrical, and optical features of F-incorporated samples were evaluated depending on the Nd loading. X-ray diffraction analysis revealed that samples had tetragonal tin oxide structure with (211) and (200) preferential directions. The crystallite size and strain values varied from 37.98 nm and 1.21 × 10-3 to 52.12 nm and 1.88 × 10-3. Scanning electron microscopy analysis showed that the samples consisted of pyramidal, polyhedral, and needle-shaped granules. The lowest sheet resistance value of 1.22 Ω was found for 1.8 at.% Nd + 25 at.% F-coloaded SnO2. However, the widest optical bandgap of 4.01 eV was observed for the single 25 at.% F-loaded sample. The Urbach tail and figure of merit also changed in the ranges of 664 meV to 1296 meV and 6.4 × 10-2 Ω-1 to 2.3 × 10-3 Ω-1, respectively. The results presented herein indicate that the character of F-doped tin oxide films can be controlled by Nd loading and that these films could be useful for technological applications.

Simultaneous whole body 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging for evaluation of pediatric cancer: Preliminary experience and comparison with 18F-fluorodeoxyglucose positron emission tomography computed tomogra

Institute of Scientific and Technical Information of China (English)

Brian S Pugmire; Alexander R Guimaraes; Ruth Lim; Alison M Friedmann; Mary Huang; David Ebb; Howard Weinstein; Onofrio A Catalano; Umar Mahmood; Ciprian Catana; Michael S Gee

2016-01-01

AIM: To describe our preliminary experience with simultaneous whole body 18F-fluorodeoxyglucose(18F-FDG)positron emission tomography and magnetic resonance imaging（PET-MRI） in the evaluation of pediatric oncology patients.METHODS: This prospective, observational, singlecenter study was Health Insurance Portability and Accountability Act-compliant, and institutional review board approved. To be eligible, a patient was required to:（1） have a known or suspected cancer diagnosis;（2） be under the care of a pediatric hematologist/oncologist; and（3） be scheduled for clinically indicated 18F-FDG PETCT examination at our institution. Patients underwent PET-CT followed by PET-MRI on the same day. PET-CT examinations were performed using standard department protocols. PET-MRI studies were acquired with an integrated 3 Tesla PET-MRI scanner using whole body T1 Dixon, T2 HASTE, EPI diffusion-weighted imaging（DWI） and STIR sequences. No additional radiotracer was given for the PET-MRI examination. Both PET-CT and PETMRI examinations were reviewed by consensus by two study personnel. Test performance characteristics of PETMRI, for the detection of malignant lesions, including FDG maximum standardized uptake value（SUVmax） and minimum apparent diffusion coefficient（ADCmin）, were calculated on a per lesion basis using PET-CT as a reference standard.RESULTS: A total of 10 whole body PET-MRI exams were performed in 7 pediatric oncology patients. The mean patient age was 16.1 years（range 12-19 years） including 6 males and 1 female. A total of 20 malignant and 21 benign lesions were identified on PET-CT. PET-MRI SUVmax had excellent correlation with PET-CT SUVmax for both benign and malignant lesions（R = 0.93）. PETMRI SUVmax > 2.5 had 100% accuracy for discriminating benign from malignant lesions using PET-computed tomography（CT） reference. Whole body DWI was also evaluated: the mean ADCmin of malignant lesions（780.2 + 326.6） was significantly

Simultaneous whole body (18)F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging for evaluation of pediatric cancer: Preliminary experience and comparison with (18)F-fluorodeoxyglucose positron emission tomography computed tomography.

Science.gov (United States)

Pugmire, Brian S; Guimaraes, Alexander R; Lim, Ruth; Friedmann, Alison M; Huang, Mary; Ebb, David; Weinstein, Howard; Catalano, Onofrio A; Mahmood, Umar; Catana, Ciprian; Gee, Michael S

2016-03-28

To describe our preliminary experience with simultaneous whole body (18)F-fluorodeoxyglucose ((18)F-FDG) positron emission tomography and magnetic resonance imaging (PET-MRI) in the evaluation of pediatric oncology patients. This prospective, observational, single-center study was Health Insurance Portability and Accountability Act-compliant, and institutional review board approved. To be eligible, a patient was required to: (1) have a known or suspected cancer diagnosis; (2) be under the care of a pediatric hematologist/oncologist; and (3) be scheduled for clinically indicated (18)F-FDG positron emission tomography-computed tomography (PET-CT) examination at our institution. Patients underwent PET-CT followed by PET-MRI on the same day. PET-CT examinations were performed using standard department protocols. PET-MRI studies were acquired with an integrated 3 Tesla PET-MRI scanner using whole body T1 Dixon, T2 HASTE, EPI diffusion-weighted imaging (DWI) and STIR sequences. No additional radiotracer was given for the PET-MRI examination. Both PET-CT and PET-MRI examinations were reviewed by consensus by two study personnel. Test performance characteristics of PET-MRI, for the detection of malignant lesions, including FDG maximum standardized uptake value (SUVmax) and minimum apparent diffusion coefficient (ADCmin), were calculated on a per lesion basis using PET-CT as a reference standard. A total of 10 whole body PET-MRI exams were performed in 7 pediatric oncology patients. The mean patient age was 16.1 years (range 12-19 years) including 6 males and 1 female. A total of 20 malignant and 21 benign lesions were identified on PET-CT. PET-MRI SUVmax had excellent correlation with PET-CT SUVmax for both benign and malignant lesions (R = 0.93). PET-MRI SUVmax > 2.5 had 100% accuracy for discriminating benign from malignant lesions using PET-CT reference. Whole body DWI was also evaluated: the mean ADCmin of malignant lesions (780.2 + 326.6) was significantly lower than

Simultaneous whole body 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging for evaluation of pediatric cancer: Preliminary experience and comparison with 18F-fluorodeoxyglucose positron emission tomography computed tomography

Science.gov (United States)

Pugmire, Brian S; Guimaraes, Alexander R; Lim, Ruth; Friedmann, Alison M; Huang, Mary; Ebb, David; Weinstein, Howard; Catalano, Onofrio A; Mahmood, Umar; Catana, Ciprian; Gee, Michael S

2016-01-01

AIM: To describe our preliminary experience with simultaneous whole body 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography and magnetic resonance imaging (PET-MRI) in the evaluation of pediatric oncology patients. METHODS: This prospective, observational, single-center study was Health Insurance Portability and Accountability Act-compliant, and institutional review board approved. To be eligible, a patient was required to: (1) have a known or suspected cancer diagnosis; (2) be under the care of a pediatric hematologist/oncologist; and (3) be scheduled for clinically indicated 18F-FDG positron emission tomography-computed tomography (PET-CT) examination at our institution. Patients underwent PET-CT followed by PET-MRI on the same day. PET-CT examinations were performed using standard department protocols. PET-MRI studies were acquired with an integrated 3 Tesla PET-MRI scanner using whole body T1 Dixon, T2 HASTE, EPI diffusion-weighted imaging (DWI) and STIR sequences. No additional radiotracer was given for the PET-MRI examination. Both PET-CT and PET-MRI examinations were reviewed by consensus by two study personnel. Test performance characteristics of PET-MRI, for the detection of malignant lesions, including FDG maximum standardized uptake value (SUVmax) and minimum apparent diffusion coefficient (ADCmin), were calculated on a per lesion basis using PET-CT as a reference standard. RESULTS: A total of 10 whole body PET-MRI exams were performed in 7 pediatric oncology patients. The mean patient age was 16.1 years (range 12-19 years) including 6 males and 1 female. A total of 20 malignant and 21 benign lesions were identified on PET-CT. PET-MRI SUVmax had excellent correlation with PET-CT SUVmax for both benign and malignant lesions (R = 0.93). PET-MRI SUVmax > 2.5 had 100% accuracy for discriminating benign from malignant lesions using PET-CT reference. Whole body DWI was also evaluated: the mean ADCmin of malignant lesions (780.2 + 326.6) was

Combined 18F-NaF and 18F-FDG PET/CT in the Evaluation of Sarcoma Patients.

Science.gov (United States)

Jackson, Tatianie; Mosci, Camila; von Eyben, Rie; Mittra, Erik; Ganjoo, Kristen; Biswal, Sandip; Gambhir, Sanjiv Sam; Iagaru, Andrei

2015-09-01

The combined administration of F-NaF and F-FDG in a single PET/CT scan has the potential to improve patient convenience and cancer detection. Here we report the use of this approach for patients with sarcomas. This is a retrospective review of 21 patients (12 men, 9 women; age, 19-66 years) with biopsy-proven sarcomas who had separate F-NaF PET/CT, F-FDG PET/CT, and combined F-NaF/F-FDG PET/CT scans for evaluation of malignancy. Two board-certified nuclear medicine physicians and 1 board-certified musculoskeletal radiologist were randomly assigned to review the scans. Results were analyzed for sensitivity and specificity, using linear regression and receiver operating characteristics. A total of 13 patients had metastatic disease on F-NaF PET/CT, F-FDG PET/CT, and combined F-NaF/F-FDG PET/CT. Skeletal disease was more extensive on the F-NaF PET/CT scan than on the F-FDG PET/CT in 3 patients, whereas in 1 patient, F-FDG PET/CT showed skeletal disease and the F-NaF PET/CT was negative. Extraskeletal lesions were detected on both F-FDG and combined F-NaF/F-FDG PET/CT in 20 patients, with 1 discordant finding in the lung. The combined F-NaF/F-FDG PET/CT scan allows for accurate evaluation of sarcoma patients. Further evaluation of this proposed imaging modality is warranted to identify the most suitable clinical scenarios, including initial treatment strategy and evaluation of response to therapy.

Verkligt värde vid första redovisningstillfället?

DEFF Research Database (Denmark)

Thinggaard, Frank; Nilsson, Sven-Arne

2007-01-01

I en första artikel av två diskuterar Frank Thinggaard och Sven-Arne Nilsson en rapport från IASB som föreslår verkligt värde istället för anskaffningsvärde vid första redovisningstillfället. Där rapporten står för nytänkande undersöker de om denär i linje med en rapport om förbättrad föreställni...

EEG-fMRI Evaluation of Patients with Mesial Temporal Lobe Sclerosis

Science.gov (United States)

Avesani, Mirko; Giacopuzzi, Silvia; Bongiovanni, Luigi Giuseppe; Borelli, Paolo; Cerini, Roberto; Pozzi Mucelli, Roberto; Fiaschi, Antonio

2014-01-01

Summary This preliminary study sought more information on blood oxygen level dependent (BOLD) activation, especially contralateral temporal/extratemporal spread, during continuous EEG-fMRI recordings in four patients with mesial temporal sclerosis (MTS). In two patients, EEG showed unilateral focal activity during the EEG-fMRI session concordant with the interictal focus previously identified with standard and video-poly EEG. In the other two patients EEG demonstrated a contralateral diffusion of the irritative focus. In the third patient (with the most drug-resistant form and also extratemporal clinical signs), there was an extratemporal diffusion over frontal regions, ipsilateral to the irritative focus. fMRI analysis confirmed a single activation in the mesial temporal region in two patients whose EEG showed unilateral focal activity, while it demonstrated a bilateral activation in the mesial temporal regions in the other two patients. In the third patient, fMRI demonstrated an activation in the supplementary motxor area. This study confirms the most significant activation with a high firing rate of the irritative focus, but also suggests the importance of using new techniques (such as EEG-fMRI to examine cerebral blood flow) to identify the controlateral limbic activation, and any other extratemporal activations, possible causes of drug resistance in MTS that may require a more precise pre-surgical evaluation with invasive techniques. PMID:24571833

A recombinant raccoon poxvirus vaccine expressing both Yersinia pestis F1 and truncated V antigens protects animals against lethal plague.

Science.gov (United States)

Rocke, Tonie E.; Kingstad-Bakke, B; Berlier, W; Osorio, J.E.

2014-01-01

In previous studies, we demonstrated in mice and prairie dogs that simultaneous administration of two recombinant raccoon poxviruses (rRCN) expressing Yersinia pestis antigens (F1 and V307-a truncated version of the V protein) provided superior protection against plague challenge compared to individual single antigen constructs. To reduce costs of vaccine production and facilitate implementation of a sylvatic plague vaccine (SPV) control program for prairie dogs, a dual antigen construct is more desirable. Here we report the construction and characterization of a novel RCN-vectored vaccine that simultaneously expresses both F1 and V307 antigens. This dual antigen vaccine provided similar levels of protection against plague in both mice and prairie dogs as compared to simultaneous administration of the two single antigen constructs and was also shown to protect mice against an F1 negative strain of Y. pestis.. The equivalent safety, immunogenicity and efficacy profile of the dual RCN-F1/V307 construct warrants further evaluation in field efficacy studies in sylvatic plague endemic areas.

V. F. Gening and problems of the Volga Bulgaria archaeology

Directory of Open Access Journals (Sweden)

Rudenko Konstantin A.

2014-03-01

Full Text Available V.F. Gening’s contribution to the study of the Volga Bulgaria and his views concerning the history and archaeology of this state formation are considered in the article. His sphere of interest first of all included the links between the Volga Bulgaria population and the tribes belonging to preceding cultures, such as Pyanoborye and Imenkovo ones. V.F. Gening investigated the Bolshie Tarkhany, Turaevo and Rozdestveno burial grounds dated by the 8-9th, 5th and 6-7th centuries A.D., and the materials collected modified the notion of the time and character of the Bulgars arrival in the Volga area. He reconsidered the chronology and interpretation of the archaeological monuments, which referred to the epoch preceding the Bulgars appearance on the Volga, and proposed a hypothesis of the Volga Bulgars as a Turkic-Ugrian ethnos. V.F. Gening created a periodization of the Bolgars history in Eastern Europe within the time span between 1st-3rd centuries AD and the early 13th century AD

Prospective evaluation of {sup 18}F-FACBC PET/CT and PET/MRI versus multiparametric MRI in intermediate- to high-risk prostate cancer patients (FLUCIPRO trial)

Energy Technology Data Exchange (ETDEWEB)

Jambor, Ivan [University of Turku, Department of Diagnostic Radiology, Turku (Finland); University of Massachusetts Medical School - Baystate, Department of Radiology, Springfield, MA (United States); Turku PET Centre, Turku (Finland); Kuisma, Anna [Turku University Hospital, Department of Oncology and Radiotherapy, Turku (Finland); Kaehkoenen, Esa; Bostroem, Peter J. [Turku University Hospital, Department of Urology, Turku (Finland); Kemppainen, Jukka [Turku PET Centre, Turku (Finland); Turku University Hospital, Department of Clinical Physiology and Nuclear Medicine, Turku (Finland); Merisaari, Harri [University of Turku, Department of Diagnostic Radiology, Turku (Finland); Turku PET Centre, Turku (Finland); University of Turku, Department of Information Technology, Turku (Finland); Eskola, Olli; Teuho, Jarmo [Turku PET Centre, Turku (Finland); Montoya Perez, Ileana [University of Turku, Department of Diagnostic Radiology, Turku (Finland); University of Turku, Department of Information Technology, Turku (Finland); Turku University Hospital, Medical Imaging Centre of Southwest Finland, Turku (Finland); Pesola, Marko [University of Turku, Department of Diagnostic Radiology, Turku (Finland); Aronen, Hannu J. [University of Turku, Department of Diagnostic Radiology, Turku (Finland); Turku University Hospital, Medical Imaging Centre of Southwest Finland, Turku (Finland); Taimen, Pekka [University of Turku and Turku University Hospital, Department of Pathology, Turku (Finland); Minn, Heikki [Turku PET Centre, Turku (Finland); Turku University Hospital, Department of Oncology and Radiotherapy, Turku (Finland)

2018-03-15

The purpose of this study was to evaluate {sup 18}F-FACBC PET/CT, PET/MRI, and multiparametric MRI (mpMRI) in detection of primary prostate cancer (PCa). Twenty-six men with histologically confirmed PCa underwent PET/CT immediately after injection of 369 ± 10 MBq {sup 18}F-FACBC (fluciclovine) followed by PET/MRI started 55 ± 7 min from injection. Maximum standardized uptake values (SUV{sub max}) were measured for both hybrid PET acquisitions. A separate mpMRI was acquired within a week of the PET scans. Logan plots were used to calculate volume of distribution (V{sub T}). The presence of PCa was estimated in 12 regions with radical prostatectomy findings as ground truth. For each imaging modality, area under the curve (AUC) for detection of PCa was determined to predict diagnostic performance. The clinical trial registration number is NCT02002455. In the visual analysis, 164/312 (53%) regions contained PCa, and 41 tumor foci were identified. PET/CT demonstrated the highest sensitivity at 87% while its specificity was low at 56%. The AUC of both PET/MRI and mpMRI significantly (p < 0.01) outperformed that of PET/CT while no differences were detected between PET/MRI and mpMRI. SUV{sub max} and V{sub T} of Gleason score (GS) >3 + 4 tumors were significantly (p < 0.05) higher than those for GS 3 + 3 and benign hyperplasia. A total of 442 lymph nodes were evaluable for staging, and PET/CT and PET/MRI demonstrated true-positive findings in only 1/7 patients with metastatic lymph nodes. Quantitative {sup 18}F-FACBC imaging significantly correlated with GS but failed to outperform MRI in lesion detection. {sup 18}F-FACBC may assist in targeted biopsies in the setting of hybrid imaging with MRI. (orig.)

Preclinical evaluation of an {sup 18}F-trifluoroborate methionine derivative for glioma imaging

Energy Technology Data Exchange (ETDEWEB)

Yang, Xiangyu [Medical School of Southeast University, Jiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Department of Radiology, Zhongda Hospital, Nanjing (China); National Institutes of Health (NIH), Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, Bethesda, MD (United States); Liu, Zhibo; Zhang, Huimin; Li, Zhu; Niu, Gang; Chen, Xiaoyuan [National Institutes of Health (NIH), Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, Bethesda, MD (United States); Munasinghe, Jeeva P. [NIH, Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke, Bethesda, MD (United States); Teng, Gaojun [Medical School of Southeast University, Jiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Department of Radiology, Zhongda Hospital, Nanjing (China)

2018-04-15

{sup 11}C-methionine (MET) is one of the most commonly used amino acid tracers for PET imaging of brain tumors. In this study, we report an {sup 18}F-labeled boron-derived methionine analogue, denoted as {sup 18}F-B-MET, as a potential substitute of {sup 11}C-MET for glioma PET imaging. {sup 19}F-B-MET was synthesized from readily available chemicals according to our previous publication. For kit development, {sup 19}F-B-MET was aliquoted in quantities of 10 nmol for on-demand one-step labeling. The {sup 18}F-labeling was performed by {sup 18}F-{sup 19}F isotope exchange, and quality control was performed by both HPLC and radio-TLC. Uptake of the tracer was determined in GL26, C6 and U87 tumor cells. PET imaging and the biodistribution assay were performed on mice bearing subcutaneous or orthotopic C6 and U87 tumor xenografts. Starting with 740-1110 MBq {sup 18}F-fluoride, >370 MBq of {sup 18}F-B-MET was obtained in 25 min (n = 5) with >99% purity and high specific activity (>37 GBq/μmol). {sup 18}F-B-MET demonstrated excellent in vitro stability with <1% decomposition after incubation with plasma for 2 h. In vitro cell uptake assay showed that {sup 18}F-B-MET accumulated in tumor cells in a time dependent manner and could be competitively inhibited by natural methionine and other L-type transporter transported amino acids. In vivo biodistribution and imaging studies showed high tumor accumulation (2.99 ± 0.23 %ID/g, n = 6) compared with low uptake of brain (0.262 ± 0.05 %ID/g, n = 6) at 60 min after injection in a subcutaneous C6 tumor model. Orthotropic C6 and U87 tumors were clearly visualized with high tumor to brain ratios at 60 min post-injection, corroborating with tumor L-type amino acid transporter 1 (LAT-1) expression levels. {sup 18}F-B-MET was radiolabeled with high yield in a one-step labeling process, showed excellent pharmacokinetic properties in vivo, with high tumor-to-brain contrast. (orig.)

Fine structure of V2+ energy levels in CsCaF3:V2+

International Nuclear Information System (INIS)

Avram, C.N.; Brik, M.G.

2004-01-01

Theoretical investigations of the fine structure of the lasing 4 T 2g level in a CsCaF 3 :V 2+ crystal were carried out. The spin-orbit splitting of the 4 T 2g term in the static low crystal field was obtained from the Eisenstein matrices and using parameters (Dq, B, C, ζ SO ) appropriate for the 4 T 2g - 4 A 2g zero-phonon line. The 4 T 2g spinor splitting has been modeled by the second-order spin-orbit Hamiltonian. The effect of the dynamical Jahn-Teller interaction on the spin-orbit splitting of the 4 T 2g term was taken into account; the Jahn-Teller stabilization energy, ZPL splitting and the Huang-Rhys parameter for the e g normal mode were all evaluated

Bim and Mcl-1 exert key roles in regulating JAK2V617F cell survival

International Nuclear Information System (INIS)

Rubert, Joëlle; Qian, Zhiyan; Andraos, Rita; Guthy, Daniel A; Radimerski, Thomas

2011-01-01

The JAK2 V617F mutation plays a major role in the pathogenesis of myeloproliferative neoplasms and is found in the vast majority of patients suffering from polycythemia vera and in roughly every second patient suffering from essential thrombocythemia or from primary myelofibrosis. The V617F mutation is thought to provide hematopoietic stem cells and myeloid progenitors with a survival and proliferation advantage. It has previously been shown that activated JAK2 promotes cell survival by upregulating the anti-apoptotic STAT5 target gene Bcl-xL. In this study, we have investigated the role of additional apoptotic players, the pro-apoptotic protein Bim as well as the anti-apoptotic protein Mcl-1. Pharmacological inhibition of JAK2/STAT5 signaling in JAK2 V617F mutant SET-2 and MB-02 cells was used to study effects on signaling, cell proliferation and apoptosis by Western blot analysis, WST-1 proliferation assays and flow cytometry. Cells were transfected with siRNA oligos to deplete candidate pro- and anti-apoptotic proteins. Co-immunoprecipitation assays were performed to assess the impact of JAK2 inhibition on complexes of pro- and anti-apoptotic proteins. Treatment of JAK2 V617F mutant cell lines with a JAK2 inhibitor was found to trigger Bim activation. Furthermore, Bim depletion by RNAi suppressed JAK2 inhibitor-induced cell death. Bim activation following JAK2 inhibition led to enhanced sequestration of Mcl-1, besides Bcl-xL. Importantly, Mcl-1 depletion by RNAi was sufficient to compromise JAK2 V617F mutant cell viability and sensitized the cells to JAK2 inhibition. We conclude that Bim and Mcl-1 have key opposing roles in regulating JAK2 V617F cell survival and propose that inactivation of aberrant JAK2 signaling leads to changes in Bim complexes that trigger cell death. Thus, further preclinical evaluation of combinations of JAK2 inhibitors with Bcl-2 family antagonists that also tackle Mcl-1, besides Bcl-xL, is warranted to assess the therapeutic potential

A Recombinant Trivalent Fusion Protein F1-LcrV-HSP70(II) Augments Humoral and Cellular Immune Responses and Imparts Full Protection against Yersinia pestis.

Science.gov (United States)

Verma, Shailendra K; Batra, Lalit; Tuteja, Urmil

2016-01-01

Plague is one of the most dangerous infections in humans caused by Yersinia pestis, a Gram-negative bacterium. Despite of an overwhelming research success, no ideal vaccine against plague is available yet. It is well established that F1/LcrV based vaccine requires a strong cellular immune response for complete protection against plague. In our earlier study, we demonstrated that HSP70(II) of Mycobacterium tuberculosis modulates the humoral and cellular immunity of F1/LcrV vaccine candidates individually as well as in combinations in a mouse model. Here, we made two recombinant constructs caf1-lcrV and caf1-lcrV-hsp70(II). The caf1 and lcrV genes of Y. pestis and hsp70 domain II of M. tuberculosis were amplified by polymerase chain reaction. Both the recombinant constructs caf1-lcrV and caf1-lcrV-hsp70(II) were cloned in pET28a vector and expressed in Escherichia coli. The recombinant fusion proteins F1-LcrV and F1-LcrV-HSP70(II) were purified using Ni-NTA columns and formulated with alum to evaluate the humoral and cell mediated immune responses in mice. The protective efficacies of F1-LcrV and F1-LcrV-HSP70(II) were determined following challenge of immunized mice with 100 LD50 of Y. pestis through intraperitoneal route. Significant differences were noticed in the titers of IgG and it's isotypes, i.e., IgG1, IgG2b, and IgG3 in anti- F1-LcrV-HSP70(II) sera in comparison to anti-F1-LcrV sera. Similarly, significant differences were also noticed in the expression levels of IL-2, IFN-γ and TNF-α in splenocytes of F1-LcrV-HSP(II) immunized mice in comparison to F1-LcrV. Both F1-LcrV and F1-LcrV-HSP70(II) provided 100% protection. Our research findings suggest that F1-LcrV fused with HSP70 domain II of M. tuberculosis significantly enhanced the humoral and cellular immune responses in mouse model.

Dissociative scattering of low-energy SiF{sub 3}{sup +} and SiF{sup +} ions (5-200 eV) on Cu(100) surface

Energy Technology Data Exchange (ETDEWEB)

Yamamoto, Hiroyuki; Baba, Yuji; Sasaki, T A [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

1997-03-01

Dissociative scattering of molecular SiF{sub 3}{sup +} and SiF{sup +} ions from a Cu(100) single crystal surface has been investigated in the incident energy range from 5 eV to 200 eV with a scattering angle of 77deg. The scattered ion intensity of dissociative ions and parent molecular ions were measured as a function of incident ion energy. The observed data show that onset energies of dissociation for SiF{sub 3}{sup +} and SiF{sup +} ions are 30 eV and 40 eV, respectively. The obtained threshold energies are consistent with a impulsive collision model where the dissociation of incident ion is caused by vibrational excitation during collision. (author)

Radiopharmacological evaluation of 18F-labeled phosphatidylserine-binding peptides for molecular imaging of apoptosis

International Nuclear Information System (INIS)

Wuest, Melinda; Perreault, Amanda; Kapty, Janice; Richter, Susan; Foerster, Christian; Bergman, Cody; Way, Jenilee; Mercer, John; Wuest, Frank

2015-01-01

Introduction: Radiolabeled phosphatidylserine (PS)-binding peptides represent an innovative strategy for molecular imaging of apoptosis with positron emission tomography (PET). The goal of this study was the radiopharmacological evaluation of radiolabeled peptides for their binding to PS on apoptotic cancer cells, involving metabolic stability, cellular uptake, biodistribution, and dynamic PET imaging experiments. Methods: Binding of peptides LIKKPF, PGDLSR, FBz-LIKKPF, FBz-PGDLSR, FBAM-CLIKKPF and FBAM-CPGDLSR to PS was analyzed in a newly developed radiometric binding assay using 64 Cu-labeled wild-type annexin-V as radiotracer. Radiolabeling of most potent peptides with fluorine-18 was carried out with thiol-selective prosthetic group [ 18 F]FBAM to give [ 18 F]FBAM-CLIKKPF and [ 18 F]FBAM-CPGDLSR. [ 18 F]FBAM-labeled peptides were studied in camptothecin-induced apoptotic human T lymphocyte Jurkat cells, and in a murine EL4 tumor model of apoptosis using dynamic PET imaging and biodistribution. Results: Peptides LIKKPF and PGDLSR inhibited binding of 64 Cu-labeled annexin-V to immobilized PS in the millimolar range (IC 50 10–15 mM) compared to annexin-V (45 nM). Introduction of FBAM prosthetic group slightly increased inhibitory potencies (FBAM-CLIKKPF: IC 50 = 1 mM; FBAM-CPGDLSR: IC 50 = 6 mM). Radiolabeling succeeded in good radiochemical yields of 50–54% using a chemoselective alkylation reaction of peptides CLIKKPF and CPGDLSR with [ 18 F]FBAM. In vivo metabolic stability studies in mice revealed 40–60% of intact peptides at 5 min p.i. decreasing to 25% for [ 18 F]FBAM-CLIKKPF and less than 5% for [ 18 F]FBAM-CPGDLSR at 15 min p.i.. Cell binding of [ 18 F]FBAM-CLIKKPF in drug-treated Jurkat cells was significantly higher compared to untreated cells, but this was not observed for [ 18 F]FBAM-CPGDLSR. Dynamic PET imaging experiments showed that baseline uptake of [ 18 F]FBAM-CLIKKPF in EL4 tumors was higher (SUV 5min 0.46, SUV 60min 0.13) compared to

Preliminary report on electron energy-loss measurements for CCl3, CCl2F2, CCl3F

International Nuclear Information System (INIS)

Bushnell, D.L. Jr.; Huebner, R.H.; Celotta, R.J.; Mielczarek, S.R.

1975-01-01

Currently, nation-wide research efforts are devoted to studying the possible ozone (O 3 ) depletion in the stratosphere by the chemical action of chlorine atoms released from CCl 2 F 2 or CCl 3 F upon absorption of ultraviolet radiation. Since electron-impact data taken in the forward scattering direction can be used to derive oscillator strengths and thus to yield apparent photoabsorption cross sections, such an analysis for CCl 2 F 2 , CCLl 3 F, and CClF 3 was carried out. Oscillator-strength distributions were obtained between 5 and 20 eV and are compared to available photoabsorption data. Certain photoabsorption values agree very well with these electron-impact data, but other optical studies deviate in some spectral regions by as much as a factor of 5. Also, the electron energy-loss spectrum reveals electronic transitions previously undetected by photoabsorption

18F-FDG PET/CT prediction of malignant versus benign lesion in patients presenting with adnexal mass

DEFF Research Database (Denmark)

Frost, Majbritt; Iyer, Victor Vishwanath; Fisker, Rune Vincents

2011-01-01

classified as benign or malignant and the results were compared with the registered intra-operative findings and the final histopathological examination. Preoperative 18F-FDG PET/CT-based staging was compared to the final histopathological staging according to FIGO 2003. Results: The preliminary results......%] and a specificity of 76 % CI: [63; 87%]. Conclusions: 18F-FDG PET/CT-scanning can be useful for preoperative differentiation between benign and malignant adnexal masses. Furthermore, as a whole-body examination with intravenous contrast, it gives useful information of metastasis extent of malignant diseases...

PET/CT studies of multiple myeloma using {sup 18}F-FDG and {sup 18}F-NaF: comparison of distribution patterns and tracers' pharmacokinetics

Energy Technology Data Exchange (ETDEWEB)

Sachpekidis, Christos [Clinical Cooperation Unit Nuclear Medicine, German Cancer Research Center, Heidelberg (Germany); German Cancer Research Center, Medical PET Group - Biological Imaging Clinical Cooperation Unit Nuclear Medicine, Heidelberg (Germany); Goldschmidt, Hartmut; Hose, Dirk [University of Heidelberg, Medical Clinic V, Heidelberg (Germany); National Center for Tumor Diseases Heidelberg, Heidelberg (Germany); Pan, Leyun; Cheng, Caixia; Dimitrakopoulou-Strauss, Antonia [Clinical Cooperation Unit Nuclear Medicine, German Cancer Research Center, Heidelberg (Germany); Kopka, Klaus [German Cancer Research Center, Division of Radiopharmaceutical Chemistry, Heidelberg (Germany); Haberkorn, Uwe [Clinical Cooperation Unit Nuclear Medicine, German Cancer Research Center, Heidelberg (Germany); University of Heidelberg, Division of Nuclear Medicine, Heidelberg (Germany)

2014-07-15

following mean values: SUV{sub aver} = 5.1, k{sub 1} = 0.37 (1/min), k{sub 3} = 0.10 (1/min), V{sub B} = 0.06, influx = 0.04 (1/min), FD = 1.28; the respective values for {sup 18}F-NaF were SUV{sub average} = 10.7, k{sub 1} = 0.25 (1/min), k{sub 3} = 0.34 (1/min), V{sub B} = 0.02, influx = 0.10 (1/min), FD = 1.37. Apart from the correlation between V{sub B} of {sup 18}F-FDG and k{sub 1} of {sup 18}F-NaF (r = 0.54), no other significant correlation was observed between the two tracers' kinetic parameters. We found a significant correlation between FD and SUV{sub average} (r = 0.93), FD and SUV{sub max} (r = 0.80), FD and influx (r = 0.85), as well as between influx and SUV{sub average} (r = 0.74) for {sup 18}F-FDG. In {sup 18}F-NaF we observed the most significant correlations between FD and SUV{sub average} (r = 0.97), FD and SUV{sub max} (r = 0.87), and between influx and k{sub 1} (r = 0.72). The combined use of {sup 18}F-FDG PET/CT and {sup 18}F-NaF PET/CT provides different molecular information regarding the biological processes that take place in a MM osseous lesion. {sup 18}F-FDG PET/CT proved to be a more specific biomarker than {sup 18}F-NaF PET/CT in multiple myeloma skeletal assessment. (orig.)

JAK2V617F mutation in chronic myeloid leukemia predicts early disease progression

International Nuclear Information System (INIS)

Pahore, Z.A.A.; Shamsi, T.S.; Taj, M.; Farzana, T.; Ansari, S.H.; Nadeem, M.; Ahmad, M.; Naz, A.

2011-01-01

Objective: To determine the association of JAK2V617F mutation along with BCR-ABL translocation or Philadelphia chromosome in chronic myeloid leukemia with early disease progression to advanced stages (accelerated phase or blast crisis) and poor outcome. Study Design: Case series. Place and Duration of Study: National Institute of Blood Diseases and Bone Marrow Transplantation, Karachi, from February 2008 to August 2009. Methodology: All the newly diagnosed cases of BCR-ABL or Philadelphia positive CML were tested for JAK2V617F mutation by Nested PCR. Demographic data, spleen size, hemoglobin levels, white blood cell and platelet counts were recorded. Independent sample t-test was used for age, haemoglobin level and spleen size. Fisher's exact test was applied to compare disease progression in JAK2V617F mutation positive and negative cases. Results: Out of 45 newly diagnosed cases of CML, 40 were in chronic phase, 01 in accelerated phase and 04 in blast crisis. JAK2V617F mutation was detected in 12 (26.7%) patients; 09 (22.5%) in chronic phase, none in accelerated phase and 03 (75%) in blast crisis. During a mean follow-up of 8 months, 03 patients in chronic phase transformed in blast crisis and 02 into accelerated phase. Overall 08 out of 11 (73%) JAK2V617F positive patients either had advanced disease or showed disease progression. Only 2 of 20 (10%) available patients, negative for the mutation, showed disease progression by transforming into blast crisis (p < 0.001). No statistically significant difference was seen in the age, spleen size, haemoglobin levels, white blood cells and platelets counts in JAK2V617F positive patients. Conclusion: JAK2V617F mutation was detected in 26.7% cases of chronic myeloid leukemia. A significant proportion of them showed early disease progression. (author)

Single shot diffraction of picosecond 8.7-keV x-ray pulses

Directory of Open Access Journals (Sweden)

F. H. O’Shea

2012-02-01

Full Text Available We demonstrate multiphoton, single shot diffraction images of x rays produced by inverse Compton scattering a high-power CO_{2} laser from a relativistic electron beam, creating a pulse of 8.7 keV x rays. The tightly focused, relatively high peak brightness electron beam and high photon density from the 2 J CO_{2} laser yielded 6×10^{7} x-ray photons over the full opening angle in a single shot. Single shot x-ray diffraction is performed by passing the x rays though a vertical slit and on to a flat silicon (111 crystal. 10^{2} diffracted photons were detected. The spectrum of the detected x rays is compared to simulation. The diffraction and detection of 10^{2} x rays is a key step to a more efficient time resolved diagnostic in which the number of observed x rays might reach 10^{4}; enabling a unique, flexible x-ray source as a sub-ps resolution diagnostic for studying the evolution of chemical reactions, lattice deformation and melting, and magnetism.

Evaluation of TSPO PET Ligands [18F]VUIIS1009A and [18F]VUIIS1009B: Tracers for Cancer Imaging.

Science.gov (United States)

Tang, Dewei; Li, Jun; Buck, Jason R; Tantawy, Mohamed Noor; Xia, Yan; Harp, Joel M; Nickels, Michael L; Meiler, Jens; Manning, H Charles

2017-08-01

Positron emission tomography (PET) ligands targeting translocator protein (TSPO) are potential imaging diagnostics of cancer. In this study, we report two novel, high-affinity TSPO PET ligands that are 5,7 regioisomers, [ 18 F]VUIIS1009A ([ 18 F]3A) and [ 18 F]VUIIS1009B ([ 18 F]3B), and their initial in vitro and in vivo evaluation in healthy mice and glioma-bearing rats. VUIIS1009A/B was synthesized and confirmed by X-ray crystallography. Interactions between TSPO binding pocket and novel ligands were evaluated and compared with contemporary TSPO ligands using 2D 1 H- 15 N heteronuclear single quantum coherence (HSQC) spectroscopy. In vivo biodistribution of [ 18 F]VUIIS1009A and [ 18 F]VUIIS1009B was carried out in healthy mice with and without radioligand displacement. Dynamic PET imaging data were acquired simultaneously with [ 18 F]VUIIS1009A/B injections in glioma-bearing rats, with binding reversibility and specificity evaluated by radioligand displacement. In vivo radiometabolite analysis was performed using radio-TLC, and quantitative analysis of PET data was performed using metabolite-corrected arterial input functions. Imaging was validated with histology and immunohistochemistry. Both VUIIS1009A (3A) and VUIIS1009B (3B) were found to exhibit exceptional binding affinity to TSPO, with observed IC 50 values against PK11195 approximately 500-fold lower than DPA-714. However, HSQC NMR suggested that VUIIS1009A and VUIIS1009B share a common binding pocket within mammalian TSPO (mTSPO) as DPA-714 and to a lesser extent, PK11195. [ 18 F]VUIIS1009A ([ 18 F]3A) and [ 18 F]VUIIS1009B ([ 18 F]3B) exhibited similar biodistribution in healthy mice. In rats bearing C6 gliomas, both [ 18 F]VUIIS1009A and [ 18 F]VUIIS1009B exhibited greater binding potential (k 3 /k 4 )in tumor tissue compared to [ 18 F]DPA-714. Interestingly, [ 18 F]VUIIS1009B exhibited significantly greater tumor uptake (V T ) than [ 18 F]VUIIS1009A, which was attributed primarily to greater plasma

Prospective evaluation of 18F-FACBC PET/CT and PET/MRI versus multiparametric MRI in intermediate- to high-risk prostate cancer patients (FLUCIPRO trial).

Science.gov (United States)

Jambor, Ivan; Kuisma, Anna; Kähkönen, Esa; Kemppainen, Jukka; Merisaari, Harri; Eskola, Olli; Teuho, Jarmo; Perez, Ileana Montoya; Pesola, Marko; Aronen, Hannu J; Boström, Peter J; Taimen, Pekka; Minn, Heikki

2018-03-01

The purpose of this study was to evaluate 18 F-FACBC PET/CT, PET/MRI, and multiparametric MRI (mpMRI) in detection of primary prostate cancer (PCa). Twenty-six men with histologically confirmed PCa underwent PET/CT immediately after injection of 369 ± 10 MBq 18 F-FACBC (fluciclovine) followed by PET/MRI started 55 ± 7 min from injection. Maximum standardized uptake values (SUV max ) were measured for both hybrid PET acquisitions. A separate mpMRI was acquired within a week of the PET scans. Logan plots were used to calculate volume of distribution (V T ). The presence of PCa was estimated in 12 regions with radical prostatectomy findings as ground truth. For each imaging modality, area under the curve (AUC) for detection of PCa was determined to predict diagnostic performance. The clinical trial registration number is NCT02002455. In the visual analysis, 164/312 (53%) regions contained PCa, and 41 tumor foci were identified. PET/CT demonstrated the highest sensitivity at 87% while its specificity was low at 56%. The AUC of both PET/MRI and mpMRI significantly (p PET/CT while no differences were detected between PET/MRI and mpMRI. SUV max and V T of Gleason score (GS) >3 + 4 tumors were significantly (p PET/CT and PET/MRI demonstrated true-positive findings in only 1/7 patients with metastatic lymph nodes. Quantitative 18 F-FACBC imaging significantly correlated with GS but failed to outperform MRI in lesion detection. 18 F-FACBC may assist in targeted biopsies in the setting of hybrid imaging with MRI.

V.F. Gening and issues on the archaeology of Volga Bulgaria

Directory of Open Access Journals (Sweden)

Rudenko Konstantin A.

2014-03-01

Full Text Available V.F. Gening’s contribution to the study of the Volga Bulgaria and his views concerning the history and archaeology of this state formation are considered in the article. His sphere of interest first of all included the links between the Volga Bulgaria population and the tribes belonging to preceding cultures, such as Pyanoborye and Imenkovo ones. V.F. Gening investigated the Bolshie Tarkhany, Turaevo and Rozdestveno burial grounds dated by the 8-9th, 5th and 6-7th centuries A.D., and the materials collected modified the notion of the time and character of the Bulgars arrival in the Volga area. He reconsidered the chronology and interpretation of the archaeological monuments, which referred to the epoch preceding the Bulgars appearance on the Volga, and proposed a hypothesis of the Volga Bulgars as a Turkic-Ugrian ethnos. V.F. Gening created a periodization of the Bolgars history in Eastern Europe within the time span between 1st-3rd centuries AD and the early 13th century AD

Direct measurement of the half-life of Rb{sup 87}; Mesure directe de la periode du rubidium-87; Pryamoe izmerenie poluraspada rubidiya-87; Medicion directa del periodo del {sup 87}Rb

Energy Technology Data Exchange (ETDEWEB)

McNair, A; Wilson, H W [United Kingdom Atomic Energy Authority, Aldermaston, Berks. (United Kingdom)

1962-01-15

especial atencion a una reduccion del espesor de la fuente y de su soporte. Los autores han recurrido a diversos procedimientos para aumentar la precision de las mediciones. El espesor de la fuente se redujo empleando: i) un contador proporcional 4 {pi} de gran superficie util (hasta 100 cm{sup 2}), ii) un circuito de anticoincidencias para disminuir la intensidad de la radiacion de fondo, y iii) {sup 87}Rb enriquecido, lo que permite reducir a la cuarta parte el espesor de la fuente para una actividad dada. De esta forma, se han podido medir fuentes hasta de 5 {mu}g/cm{sup 2}. Tambien examinaron la relacion entre el periodo de semidesintegracion y el espesor de la fuente, a fin de poder introducir una correccion para tener en cuenta la debil absorcion remanente. El efecto del espesor del soporte de la fuente no estan considerable y puede calcularse partiendo: i) de la diferencia entre la velocidad de recuento en ambos lados de los delgados soportes utilizados, y ii) de un estudio de la relacion existente entre el periodo de semidesintegracion y el espesor del soporte de la fuente. Estos experimentos dan un valor aproximado de 5,25 x 10{sup 12} anos para el periodo de semidesintegracion del {sup 87}Rb. (author) [Russian] Period poluraspada Rb''8''7 izmeryalsya neposredstvenno putem opredeleniya spetsificheskoj aktivnosti metodom scheta. Poluchennye ranee periody poluraspada nakhodyatsya v predelakh 4 - 6 kh 10''1''0 let s velichinoj, kotoraya beretsya obychno v 5 kh 10''1''0 let. Metod pryamogo scheta yavlyaetsya dovol'no slozhnym iz-za nalichiya bol'shogo chisla ehlektronov ochen' maloj ehnergii v spektre rubidiya-87. Odnako ves'ma aktual'nym yavlyaetsya poluchenie tochnoj velichiny metodom scheta, t.e. to, chto avtory pytalis' prodelat'. V ehtikh izmereniyakh sleduet udelit' osoboe vnimanie umen'sheniyu tolshchiny istochnika i ego podkladki. Dlya uvelicheniya tochnosti izmerenij byli ispol'zovany sleduyushchie metody. Tolshchina istochnika umen'shalas' putem ispol

Specific phobia: a review of DSM-IV specific phobia and preliminary recommendations for DSM-V.

Science.gov (United States)

LeBeau, Richard T; Glenn, Daniel; Liao, Betty; Wittchen, Hans-Ulrich; Beesdo-Baum, Katja; Ollendick, Thomas; Craske, Michelle G

2010-02-01

The present review was conducted in order to evaluate the current diagnostic criteria for specific phobia (SP) in light of the empirical evidence gathered since DSM-IV and to propose changes to DSM-V where change is clearly and reliably indicated by the evidence. In response to questions put forth by the DSM-V Anxiety, OC Spectrum, Posttraumatic, and Dissociative Disorder Work Group, four primary areas were determined for this review: the accuracy and utility of the current SP type classification system, the validity of test anxiety as a type of SP, the boundary between agoraphobia and SP, and the reliability and utility of the diagnostic criteria for SP. Developmental issues are addressed within each area. Literature reviews examining academic findings published between 1994 and 2009 were carried out and the results are included herein. The review presents a number of options and preliminary recommendations to be considered for DSM-V. All of these recommendations should be considered tentative as they await the field trials and expert consensus necessary prior to their inclusion in the DSM-V. The present review also reveals a great need for future research in the area of SP and directions for such research is provided.

Employer branding på Q-Med : En fallstudie i hur företagsspecifika värden förmedlas till potentiella och befintliga medarbetare

OpenAIRE

Nilsson, Stina; Ljungmark, Erik

2011-01-01

Sammanfattning "Employer branding på Q-Med – En fallstudie i hur företagsspecifika värden förmedlas till potentiella och befintliga medarbetare" Datum: 19 januari, 2011 Nivå: Kandidatuppsats i företagsekonomi, 15 ECTS Institution: Akademin för hållbar samhälls‐ och teknikutveckling, HST, Mälardalens Högskola Författare: Erik Ljungmark 29 januari 1980, Stina Nilsson 25 april 1983 Titel: Employer branding på Q-Med – En fallstudie i hur företagsspecifika värden förmedlas till potentiella och...

Impact of JAK2V617F Mutational Status on Phenotypic Features in Essential Thrombocythemia and Primary Myelofibrosis

Directory of Open Access Journals (Sweden)

İpek Yönal

2016-05-01

Full Text Available Objective: The JAK2V617F mutation is present in the majority of patients with essential thrombocythemia (ET and primary myelofibrosis (PMF. The impact of this mutation on disease phenotype in ET and PMF is still a matter of discussion. This study aims to determine whether there are differences in clinical presentation and disease outcome between ET and PMF patients with and without the JAK2V617F mutation. Materials and Methods: In this single-center study, a total of 184 consecutive Philadelphia-negative chronic myeloproliferative neoplasms, 107 cases of ET and 77 cases of PMF, were genotyped for JAK2V617F mutation using the JAK2 Ipsogen MutaScreen assay, which involves allele-specific polymerase chain reaction. Results: ET patients positive for JAK2V617F mutation had higher hemoglobin (Hb and hematocrit (Hct levels, lower platelet counts, and more prevalent splenomegaly at diagnosis compared to patients negative for the JAK2V617F mutation, but rates of major thrombotic events, arterial thrombosis, and venous thrombosis were comparable between the groups. At presentation, PMF patients with JAK2V617F mutation had significantly higher Hb and Hct levels and leukocyte counts than patients without the mutation. Similar to the findings of ET patients, thromboembolic rates were similar in PMF patients with and without theJAK2V617F mutation. For ET and PMF patients, no difference was observed in rates of death with respect to JAK2V617F mutational status. Moreover, leukemic transformation rate was not different in our PMF patients with and without JAK2V617F mutation. Conclusion: We conclude that JAK2V617F-mutated ET patients express a polycythemia vera-like phenotype and JAK2V617F mutation in PMF patients is associated with a more pronounced myeloproliferative phenotype.

The thrombopoietin receptor, MPL, is critical for development of a JAK2V617F-induced myeloproliferative neoplasm.

Science.gov (United States)

Sangkhae, Veena; Etheridge, S Leah; Kaushansky, Kenneth; Hitchcock, Ian S

2014-12-18

The most frequent contributing factor in Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) is the acquisition of a V617F mutation in Janus kinase 2 (JAK2) in hematopoietic stem cells (HSCs). Recent evidence has demonstrated that to drive MPN transformation, JAK2V617F needs to directly associate with a functional homodimeric type I cytokine receptor, suggesting that, although acquiring JAK2V617F may promote disease, there are additional cellular components necessary for MPN development. Here we show that loss of the thrombopoietin (TPO) receptor (MPL) significantly ameliorates MPN development in JAK2V617F(+) transgenic mice, whereas loss of TPO only mildly affects the disease phenotype. Specifically, compared with JAK2V617F(+) mice, JAK2V617F(+)Mpl(-/-) mice exhibited reduced thrombocythemia, neutrophilia, splenomegaly, and neoplastic stem cell pool. The importance of MPL is highlighted as JAK2V617FMpl(+/-) mice displayed a significantly reduced MPN phenotype, indicating that Mpl level may have a substantial effect on MPN development and severity. Splenomegaly and the increased neoplastic stem cell pool were retained in JAK2V617F(+)Tpo(-/-) mice, although thrombocytosis was reduced compared with JAK2V617F(+) mice. These results demonstrate that Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) MPNs, highlighting an entirely novel target for therapeutic intervention. © 2014 by The American Society of Hematology.

Development of an HPLC method for the radiochemical purity evaluation of [18F]Fluoroestradiol

International Nuclear Information System (INIS)

Bispo, Ana Carolina de A.; Nascimento, Leonardo T.C. do; Costa, Flávia M.; Silva, Juliana B. da; Mamede, Marcelo

2017-01-01

18 F-Fluoroestradiol ([ 18 F]FES), an estrogen analog, is a radiopharmaceutical used in Positron Emission Tomography (PET) that allows evaluating the tumor cell receptor profile and the best therapy strategy, the staging, the prognosis and the response to therapy in several breast cancer cases. As there is not any pharmacopoeia's monograph of [ 18 F]FES to standardize its quality control criteria, this work presents a new HPCL's method to perform the [ 18 F]FES radiochemical purity. A liquid chromatograph was used with radioactivity and ultraviolet detectors. Three concentrations of fluoroestradiol standard solution were used along the test. Their retention time was compared to its relative radiolabelled analogue to confirm its identity. Several mobile phases with acetonitrile and two mobile phase flows were tested to optimize the runs. Peaks symmetry, retention time, theoretical plates and resolution were analyzed to choose the best conditions. The mean retention time of both standard Fluoroestradiol and [ 18 F]FES solutions were the same, demonstrating that [ 18 F]FES formulation did not interfere with [ 18 F]FES analysis. The best conditions were 1.2 mL/min and isocratic 40% V/V acetonitrile in water, which gave [ 18 F]FES peak resolution greater than 6 and symmetry factor of 1. Thus, the developed method is ready to be validated and implemented in [ 18 F]FES quality control routine in CDTN/Brazil. (author)

QTLs and potential candidate genes for heat stress tolerance identified from the mapping populations specifically segregating for F_v/F_m in wheat

DEFF Research Database (Denmark)

Sharma, Dew Kumari; Torp, Anna Maria; Rosenqvist, Eva

2017-01-01

Despite the fact that F-v/F-m (maximum quantum efficiency of photosystem II) is the most widely used parameter for a rapid non-destructive measure of stress detection in plants, there are barely any studies on the genetic understanding of this trait under heat stress. Our aim was to identify...... quantitative trait locus (QTL) and the potential candidate genes linked to F-v/F-m for improved photosynthesis under heat stress in wheat (Triticum aestivum L.). Three bi-parental F-2 mapping populations were generated by crossing three heat tolerant male parents (origin: Afghanistan and Pakistan) selected...... for high F-v/F-m with a common heat susceptible female parent (origin: Germany) selected for lowest F-v/F-m out of a pool of 1274 wheat cultivars of diverse geographic origin. Parents together with 140 F-2 individuals in each population were phenotyped by F-v/F-m under heat stress (40 degrees C for 3 days...

An unusual cysteine VL87 affects the antibody fragment conformations without interfering with the disulfide bond formation.

Science.gov (United States)

Attallah, Carolina; Aguilar, María Fernanda; Garay, A Sergio; Herrera, Fernando E; Etcheverrigaray, Marina; Oggero, Marcos; Rodrigues, Daniel E

2017-10-01

The Cys residues are almost perfectly conserved in all antibodies. They contribute significantly to the antibody fragment stability. The relevance of two natural contiguous Cys residues of an anti-recombinant human-follicle stimulation hormone (rhFSH) in a format of single-chain variable fragment (scFv) was studied. This scFv contains 5 Cys residues: V H 22 and V H 92 in the variable heavy chain (V H ) and V L 23, V L 87 and V L 88 in the variable light chain (V L ). The influence of two unusual contiguous Cys at positions V L 87 and V L 88 was studied by considering the wild type fragment and mutant variants: V L -C88S, V L -C87S, V L -C87Y. The analysis was carried out using antigen-binding ability measurement by indirect specific ELISA and a detailed molecular modeling that comprises homology methods, long molecular dynamics simulations and docking. We found that V L -C87 affected the antibody fragment stability without interfering with the disulfide bond formation. The effect of mutating the V L -C87 by a usual residue at this position like Tyr caused distant structural changes at the V H region that confers a higher mobility to the V H -CDR2 and V H -CDR3 loops improving the scFv binding to the antigen. Copyright © 2017 Elsevier Ltd. All rights reserved.

A possible approach to 14MeV neutron moderation: A preliminary study case.

Science.gov (United States)

Flammini, D; Pilotti, R; Pietropaolo, A

2017-07-01

Deuterium-Tritium (D-T) interactions produce almost monochromatic neutrons with about 14MeV energy. These neutrons are used in benchmark experiments as well as for neutron cross sections assessment in fusion reactors technology. The possibility to moderate 14MeV neutrons for purposes beyond fusion is worth to be studied in relation to projects of intense D-T sources. In this preliminary study, carried out using the MCNP Monte Carlo code, the moderation of 14MeV neutrons is approached foreseeing the use of combination of metallic materials as pre-moderator and reflectors coupled to standard water moderators. Copyright © 2017 Elsevier Ltd. All rights reserved.

Molecular genetic tests for JAK2V617F, Exon12_JAK2 and MPLW515K/L are highly informative in the evaluation of patients suspected to have BCR-ABL1-negative myeloproliferative neoplasms.

Science.gov (United States)

dos Santos, Marcos Tadeu; Mitne-Neto, Miguel; Miyashiro, Kozue; Chauffaille, Maria de Lourdes L Ferrari; Rizzatti, Edgar Gil

2014-02-01

Polycythaemia vera (PV), essential thrombocythemia (ET) and idiopathic myelofibrosis (MF), are the most common myeloproliferative neoplasms (MPN) in patients without the BCR-ABL1 gene rearrangement. They are caused by clonal expansion of haematopoietic stem cells and share, as a diagnostic criterion, the identification of JAK2V617F mutation. Classically, when other clinical criteria are present, a JAK2V617F negative case requires the analysis of Exon12_JAK2 for the diagnosis of PV, and of MPL515K/L mutations for the diagnosis of ET and MF. Here, we evaluated 78 samples from Brazilian patients suspected to have MPN, without stratification for PV, ET or MF. We found that 28 (35.9%) are JAK2V617F carriers; from the 50 remaining samples, one (2%) showed an Exon12_JAK2 mutation, and another (2%) was positive for MPLW515L mutation. In summary, the investigation of JAK2V617F, Exon12_JAK2 and MPLW515K/L was relevant for the diagnosis of 38.4% of patients suspected to have BCR-ABL1-negative MPN, suggesting that molecular genetic tests are useful for a quick and unequivocal diagnosis of MPN.

Effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts.

Science.gov (United States)

Arlen, Philip A; Singleton, Michael; Adamovicz, Jeffrey J; Ding, Yi; Davoodi-Semiromi, Abdolreza; Daniell, Henry

2008-08-01

The chloroplast bioreactor is an alternative to fermentation-based systems for production of vaccine antigens and biopharmaceuticals. We report here expression of the plague F1-V fusion antigen in chloroplasts. Site-specific transgene integration and homoplasmy were confirmed by PCR and Southern blotting. Mature leaves showed the highest level of transgene expression on the third day of continuous illumination, with a maximum level of 14.8% of the total soluble protein. Swiss Webster mice were primed with adjuvant-containing subcutaneous (s.c.) doses of F1-V and then boosted with either adjuvanted s.c. doses (s.c. F1-V mice) or unadjuvanted oral doses (oral F1-V mice). Oral F1-V mice had higher prechallenge serum immunoglobulin G1 (IgG1) titers than s.c. F1-V mice. The corresponding serum levels of antigen-specific IgG2a and IgA were 2 and 3 orders of magnitude lower, respectively. After vaccination, mice were exposed to an inhaled dose of 1.02 x 10(6) CFU of aerosolized Yersinia pestis CO92 (50% lethal dose, 6.8 x 10(4) CFU). All control animals died within 3 days. F1-V given s.c. (with adjuvant) protected 33% of the immunized mice, while 88% of the oral F1-V mice survived aerosolized Y. pestis challenge. A comparison of splenic Y. pestis CFU counts showed that there was a 7- to 10-log reduction in the mean bacterial burden in survivors. Taken together, these data indicate that oral booster doses effectively elicit protective immune responses in vivo. In addition, this is the first report of a plant-derived oral vaccine that protected animals from live Y. pestis challenge, bringing the likelihood of lower-cost vaccines closer to reality.

A Java-based fMRI processing pipeline evaluation system for assessment of univariate general linear model and multivariate canonical variate analysis-based pipelines.

Science.gov (United States)

Zhang, Jing; Liang, Lichen; Anderson, Jon R; Gatewood, Lael; Rottenberg, David A; Strother, Stephen C

2008-01-01

As functional magnetic resonance imaging (fMRI) becomes widely used, the demands for evaluation of fMRI processing pipelines and validation of fMRI analysis results is increasing rapidly. The current NPAIRS package, an IDL-based fMRI processing pipeline evaluation framework, lacks system interoperability and the ability to evaluate general linear model (GLM)-based pipelines using prediction metrics. Thus, it can not fully evaluate fMRI analytical software modules such as FSL.FEAT and NPAIRS.GLM. In order to overcome these limitations, a Java-based fMRI processing pipeline evaluation system was developed. It integrated YALE (a machine learning environment) into Fiswidgets (a fMRI software environment) to obtain system interoperability and applied an algorithm to measure GLM prediction accuracy. The results demonstrated that the system can evaluate fMRI processing pipelines with univariate GLM and multivariate canonical variates analysis (CVA)-based models on real fMRI data based on prediction accuracy (classification accuracy) and statistical parametric image (SPI) reproducibility. In addition, a preliminary study was performed where four fMRI processing pipelines with GLM and CVA modules such as FSL.FEAT and NPAIRS.CVA were evaluated with the system. The results indicated that (1) the system can compare different fMRI processing pipelines with heterogeneous models (NPAIRS.GLM, NPAIRS.CVA and FSL.FEAT) and rank their performance by automatic performance scoring, and (2) the rank of pipeline performance is highly dependent on the preprocessing operations. These results suggest that the system will be of value for the comparison, validation, standardization and optimization of functional neuroimaging software packages and fMRI processing pipelines.

Development of the high-level waste high-temperature melter feed preparation flowsheet for vitrification process testing

International Nuclear Information System (INIS)

Seymour, R.G.

1995-01-01

High-level waste (HLW) feed preparation flowsheet development was initiated in fiscal year (FY) 1994 to evaluate alternative flowsheets for preparing melter feed for high-temperature melter (HTM) vitrification testing. Three flowsheets were proposed that might lead to increased processing capacity relative to the Hanford Waste Vitrification Plant (HWVP) and that were flexible enough to use with other HLW melter technologies. This document describes the decision path that led to the selection of flowsheets to be tested in the FY 1994 small-scale HTM tests. Feed preparation flowsheet development for the HLW HTM was based on the feed preparation flowsheet that was developed for the HWVP. This approach allowed the HLW program to build upon the extensive feed preparation flowsheet database developed under the HWVP Project. Primary adjustments to the HWVP flowsheet were to the acid adjustment and glass component additions. Developmental background regarding the individual features of the HLW feed preparation flowsheets is provided. Applicability of the HWVP flowsheet features to the new HLW vitrification mission is discussed. The proposed flowsheets were tested at the laboratory-scale at Pacific Northwest Laboratory. Based on the results of this testing and previously established criteria, a reductant-based flowsheet using glycolic acid and a nitric acid-based flowsheet were selected for the FY 1994 small-scale HTM testing

A Preliminary Verification and Validation (V and V) Methodology for the Artifacts Programmed with a Hardware Description Language (HDL)

International Nuclear Information System (INIS)

Suh, Yong Suk; Keum, Jong Yong; Park, Je Youn; Jo, Ki Ho; Jo, Chang Whan

2008-01-01

Nowadays, the FPGA (Field Programmable Gate Array) is widely used in various fields of industry. The FPGA was evolved from the technology of PLD (Programmable Logic Device). The FPGA provides more logic gates than the PLD, which integrates millions of programmable logic gates into a chip. It also provides a massive, fast and reliable processing performance. So, we can integrate a system's functions into a FPGA, which can be a SoC (System on Chip). Furthermore, we can make a FPGA-based DSP, which DSP functions are implemented with a FPGA. With these merits, the FPGA is also used in the nuclear industry. For example, the safety-critical I and C component is manufactured with the FPGA. The FPGA is programmed with a HDL. The quality of the artifacts programmed with a HDL can impact on the quality of a FPGA. When a hazard fault exists in the artifact of a FPGA and is activated during its operation, an accident caused by the fault in the FPGA occurs. So, it is necessary to ensure the quality of the artifacts. This paper, for the purpose of applying it to the SMART (System-integrated Modular Advanced ReacTor) MMIS project, is to present a preliminary V and V methodology for HDL programmed artifacts. For this, we reviewed the following items: - Characteristics of HDL programming - Applicable requirements for a HDL program used for the safety-critical systems - Fault modes of a FPGA Based on the review, we establish the preliminary V and V methodology

WP5 Evaluation: D54-D55 Evaluation Results V2 (V3)

NARCIS (Netherlands)

Van Rosmalen, Peter

2011-01-01

Van Rosmalen, P. (2010, 19 May). WP5 Evaluation: D54-D55 Evaluation Results V2 (V3). Presentation at idSpace Final Review, Heerlen, The Netherlands: Open University of the Netherlands. idSpace-project.

Preliminary evaluation of FY98 KALIMER shielding design

Energy Technology Data Exchange (ETDEWEB)

Yoo, Jae Woon; Kang, Chang Mu; Kim, Young Jin [Korea Atomic Energy Research Institute, Taejon (Korea)

1999-01-01

This report describes a preliminary evaluation of the shielding design of FY98 KALIMER. The KALIMER shielding design includes the Inner Fixed Shield of a stainless cylinder located inside the support barrel; the Radial PSDRS Shields which are three B{sub 4}C cylinders located outside the support barrel at core level; the Lower IHX shield of a cylindrical B{sub 4}C plate located above the flow guide; and Inner and Outer IHX shields of B{sub 4}C cylinders located inside and outside of the support barrel, respectively. The DORT3.1 two-dimensional transport code was used to evaluate the KALIMER shielding design. The reactor system was represented by four axial zones, each of which was modeled in the R-Z geometry. The KAFAX-F22 library was used in the analyses, which was generated from the JEF-2.2 of OECD/NEA files for LMR applications by KAERI. The performance of the KALIMER shielding design is compared against the shielding design criteria. The results indicate that the support barrel, upper grid plate, and other reactor structures meet the maximum neutron fluence and DPA limits established in the shielding design criteria. Activities of the air effluent in the PSDRS were also evaluated and are shown to satisfy the maximum permissible concentration (MPC) limits in 10 CFR Part 20. In the future, the validation of the DORT model by a detailed three dimensional calculation such as MCNP and the justification of the current shielding design limits are needed. (author). 13 refs., 23 figs., 31 tabs.

Prevention of pneumonic plague in mice, rats, guinea pigs and non-human primates with clinical grade rV10, rV10-2 or F1-V vaccines

Science.gov (United States)

Quenee, Lauriane E.; Ciletti, Nancy A.; Elli, Derek; Hermanas, Timothy M.; Schneewind, Olaf

2012-01-01

Yersinia pestis causes plague, a disease with high mortality in humans that can be transmitted by fleabite or aerosol. A US Food and Drug Administration (FDA)-licensed plague vaccine is currently not available. Vaccine developers have focused on two subunits of Y. pestis: LcrV, a protein at the tip of type III secretion needles, and F1, the fraction 1 pilus antigen. F1-V, a hybrid generated via translational fusion of both antigens, is being developed for licensure as a plague vaccine. The rV10 vaccine is a non-toxigenic variant of LcrV lacking residues 271–300. Here we developed Current Good Manufacturing Practice (cGMP) protocols for rV10. Comparison of clinical grade rV10 with F1-V did not reveal significant differences in plague protection in mice, guinea pigs or cynomolgus macaques. We also developed cGMP protocols for rV10-2, a variant of rV10 with an altered affinity tag. Immunization with rV10-2 adsorbed to aluminum hydroxide elicited antibodies against LcrV and conferred pneumonic plague protection in mice, rats, guinea pigs, cynomolgus macaques and African Green monkeys. The data support further development of rV10-2 for FDA Investigational New Drug (IND) authorization review and clinical testing. PMID:21763383

On the chemical resolution of the {sup 87}Rb{sup +} (s{sup 0})/{sup 87}Sr{sup +} (s{sup 1}) isobaric interference: A kinetic search for an optimum reagent

Energy Technology Data Exchange (ETDEWEB)

Cheng Ping; Koyanagi, Gregory K. [Department of Chemistry, Centre for Research in Mass Spectrometry and Centre for Research in Earth and Space Science, York University, Toronto, Ontario, M3J 1P3 (Canada); Bohme, Diethard K. [Department of Chemistry, Centre for Research in Mass Spectrometry and Centre for Research in Earth and Space Science, York University, Toronto, Ontario, M3J 1P3 (Canada)], E-mail: dkbohme@yorku.ca

2008-10-03

Room-temperature reactions of the atomic cations Sr{sup +} and Rb{sup +} have been surveyed systematically with a variety of gases using an Inductively-Coupled Plasma/Selected-Ion Flow Tube (ICP/SIFT) tandem mass spectrometer. Rate coefficients and product distributions have been measured in He buffer gas at 0.35 Torr and 295 K for reactions of Sr{sup +} and Rb{sup +} with CH{sub 3}F, CH{sub 3}Cl, N{sub 2}O, CO{sub 2}, CS{sub 2}, SF{sub 6}, D{sub 2}O and NH{sub 3}. Rb{sup +} (s{sup 0}) is seen to be quite inert with these molecules and reacts either slowly by molecule addition or not at all, while Sr{sup +} (s{sup 1}) is much more reactive with all these 8 molecules, especially with CH{sub 3}F, CH{sub 3}Cl, N{sub 2}O and SF{sub 6}. Sr{sup +} reacts with CH{sub 3}F and SF{sub 6} by F-atom transfer, with CH{sub 3}Cl by Cl-atom transfer and with N{sub 2}O by O-atom transfer, and the reaction rate coefficients are all quite high, k {>=} 1.4 x 10{sup -11} cm{sup 3} molecules{sup -1} s{sup -1}. The extreme differences in reactivity with CH{sub 3}F, SF{sub 6}, CH{sub 3}Cl and N{sub 2}O provide a chemical basis for the separation of isobaric interferences of {sup 87}Rb{sup +} and {sup 87}Sr{sup +} often encountered in ICP-MS. Among these four molecules, SF{sub 6} exhibits the largest difference in reactivity, almost a factor of 10{sup 4}, and so is identified as the kinetically recommended reagent for the chemical resolution of the isobaric interference of {sup 87}Rb{sup +} and {sup 87}Sr{sup +}.

Evaluation of Prostate Cancer Bone Metastases with 18F-NaF and 18F-Fluorocholine PET/CT.

Science.gov (United States)

Beheshti, Mohsen; Rezaee, Alireza; Geinitz, Hans; Loidl, Wolfgang; Pirich, Christian; Langsteger, Werner

2016-10-01

18 F-fluorocholine is a specific promising agent for imaging tumor cell proliferation, particularly in prostate cancer, using PET/CT. It is a beneficial tool in the early detection of marrow-based metastases because it excludes distant metastases and evaluates the response to hormone therapy. In addition, 18 F-fluorocholine has the potential to differentiate between degenerative and malignant osseous abnormalities because degenerative changes are not choline-avid; however, the agent may accumulate in recent traumatic bony lesions. On the other hand, 18 F-NaF PET/CT can indicate increased bone turnover and is generally used in the assessment of primary and secondary osseous malignancies, the evaluation of response to treatment, and the clarification of abnormalities on other imaging modalities or clinical data. 18 F-NaF PET/CT is a highly sensitive method in the evaluation of bone metastases from prostate cancer, but it has problematic specificity, mainly because of tracer accumulation in degenerative and inflammatory bone diseases. In summary, 18 F-NaF PET/CT is a highly sensitive method, but 18 F-fluorocholine PET/CT can detect early bone marrow metastases and provide greater specificity in the detection of bone metastases in patients with prostate cancer. However, the difference seems not to be significant. © 2016 by the Society of Nuclear Medicine and Molecular Imaging, Inc.

Preliminary analysis of alternative fuel cycles for proliferation evaluation

Energy Technology Data Exchange (ETDEWEB)

Steindler, M. J.; Ripfel, H. C.F.; Rainey, R. H.

1977-01-01

The ERDA Division of Nuclear Research and Applications proposed 67 nuclear fuel cycles for assessment as to their nonproliferation potential. The object of the assessment was to determine which fuel cycles pose inherently low risk for nuclear weapon proliferation while retaining the major benefits of nuclear energy. This report is a preliminary analysis of these fuel cycles to develop the fuel-recycle data that will complement reactor data, environmental data, and political considerations, which must be included in the overall evaluation. This report presents the preliminary evaluations from ANL, HEDL, ORNL, and SRL and is the basis for a continuing in-depth study. (DLC)

The dimeric [V2O2F8]4− anion: Structural characterization of a magnetic basic-building-unit

International Nuclear Information System (INIS)

Lu, Hongcheng; Gautier, Romain; Li, Zuo-Xi; Jie, Wanqi; Liu, Zhengtang; Poeppelmeier, Kenneth R.

2013-01-01

New materials built from the [V 2 O 2 F 8 ] 4− anionic basic-building-unit (BBU) exhibit interesting magnetic properties owing to the proximity of the two d 1 V(IV) cations and the orbital interactions of fluoride and oxide ligands. In our search to target such materials, the vanadium oxide–fluoride compound [dpaH 2 ] 2 [V 2 O 2 F 8 ] in which a dimeric anion [V 2 O 2 F 8 ] 4− is isolated in a hydrogen bond network was hydrothermally synthesized (dpa=2,2′-dipyridylamine). This hydrogen bond network is able to stabilize the highly ionic species [V 2 O 2 F 8 ] 4− as demonstrated with bond valence calculations. The coordination of the O 2− /F − ordered ligands was investigated and antiferromagnetic coupling of the isolated BBU was measured. - The new hybrid compound [dpaH 2 ] 2 [V 2 O 2 F 8 ] built from the interesting [V 2 O 2 F 8 ] 4− magnetic basic-building-unit (BBU) was synthesized by the hydrothermal method. The coordination of the O 2− /F − ordered ligands was investigated by BVS calculations and antiferromagnetic coupling was measured. Highlights: ► A new vanadium oxyfluoride was synthesized by hydrothermal method. ► The Dimeric [V 2 O 2 F 8 ] 4− basic building unit is isolated in the hydrogen bond networks. ► The coordination of [V 2 O 2 F 8 ] 4− units to the extended structure is investigated. ► Isolated [V 2 O 2 F 8 ] 4− units exhibit antiferromagnetic coupling

Frequency of JAK2 V617F mutation in patients with Philadelphia positive Chronic Myeloid Leukemia in Pakistan.

Science.gov (United States)

Tabassum, Najia; Saboor, Mohammed; Ghani, Rubina; Moinuddin, Moinuddin

2014-01-01

Co-existence of myeloproliferative disorders (MPD) and Janus associated kinase 2 mutation (JAK2 V617F) is a well-established fact. Only few case reports are available showing presence of JAK2 V617F mutation in chronic myeloid leukemia (CML). Purpose of this study was to determine the frequency of JAK2 V617F mutation in Philadelphia Chromosome positive (Ph (+)) CML patients in Pakistan. The study was conducted from August 2009 to July 2010 at Civil Hospital and Baqai Institute of Hematology (BIH) Karachi. Blood samples from 25 patients with CML were collected. Multiplex reverse transcription polymerase chain reaction (RT-PCR) was performed for Breakpoint Cluster Region - Abelson (BCR-ABL) rearrangement. Conventional PCR was performed for JAK2 V617F mutation on BCR-ABL positive samples. All 25 samples showed BCR-ABL rearrangement. Out of these 11 samples (44%) had JAK2 V617F mutation; the remaining 14 (56%) cases showed JAK2 617V wild type. It is concluded that the co-existence of Ph (+)CML and JAK2 V617F mutation is possible.

Discovery and characterization of LY2784544, a small-molecule tyrosine kinase inhibitor of JAK2V617F

International Nuclear Information System (INIS)

Ma, L; Clayton, J R; Walgren, R A; Zhao, B; Evans, R J; Smith, M C; Heinz-Taheny, K M; Kreklau, E L; Bloem, L; Pitou, C; Shen, W; Strelow, J M; Halstead, C; Rempala, M E; Parthasarathy, S; Gillig, J R; Heinz, L J; Pei, H; Wang, Y; Stancato, L F; Dowless, M S; Iversen, P W; Burkholder, T P

2013-01-01

Owing to the prevalence of the JAK2V617F mutation in myeloproliferative neoplasms (MPNs), its constitutive activity, and ability to recapitulate the MPN phenotype in mouse models, JAK2V617F kinase is an attractive therapeutic target. We report the discovery and initial characterization of the orally bioavailable imidazopyridazine, LY2784544, a potent, selective and ATP-competitive inhibitor of janus kinase 2 (JAK2) tyrosine kinase. LY2784544 was discovered and characterized using a JAK2-inhibition screening assay in tandem with biochemical and cell-based assays. LY2784544 in vitro selectivity for JAK2 was found to be equal or superior to known JAK2 inhibitors. Further studies showed that LY2784544 effectively inhibited JAK2V617F-driven signaling and cell proliferation in Ba/F3 cells (IC 50 =20 and 55 nM, respectively). In comparison, LY2784544 was much less potent at inhibiting interleukin-3-stimulated wild-type JAK2-mediated signaling and cell proliferation (IC 50 =1183 and 1309 nM, respectively). In vivo, LY2784544 effectively inhibited STAT5 phosphorylation in Ba/F3-JAK2V617F-GFP (green fluorescent protein) ascitic tumor cells (TED 50 =12.7 mg/kg) and significantly reduced (P<0.05) Ba/F3-JAK2V617F-GFP tumor burden in the JAK2V617F-induced MPN model (TED 50 =13.7 mg/kg, twice daily). In contrast, LY2784544 showed no effect on erythroid progenitors, reticulocytes or platelets. These data suggest that LY2784544 has potential for development as a targeted agent against JAK2V617F and may have properties that allow suppression of JAK2V617F-induced MPN pathogenesis while minimizing effects on hematopoietic progenitor cells

Preliminary evaluation of the expected radiation damage of the bayonet IFMIF back-plate

Energy Technology Data Exchange (ETDEWEB)

Frisoni, M. [Athena s.a.s, Via del Battiferro 3, I-40129 Bologna (Italy)], E-mail: manuela.frisoni@enea.it; Agostini, P. [ENEA CR Brasimone, Bacino del Brasimone 40032, Camugnano (Bolivia, Plurinational State of) (Italy); Fasanella, D. [Athena s.a.s, Via del Battiferro 3, I-40129 Bologna (Italy); Micciche, G. [ENEA CR Brasimone, Bacino del Brasimone 40032, Camugnano (Bolivia, Plurinational State of) (Italy)

2009-06-15

This paper summarises and discusses the results of a preliminary damage assessment of the non-seizure coating of the bayonet IFMIF back-plate. Neutron-induced kerma factors, dpa and gas production cross sections libraries were produced in a multigroup structure for neutron energies up to 60 MeV, by processing evaluated nuclear data files with NJOY-99.259 system. The material damage evaluations in terms of heat deposition, displacement and gas production rates were calculated using these libraries and compared with the values obtained using the data contained in the pointwise ACE format files of MCNP5 code package. The calculations were performed with MCNP5 code both using the McEnea and the McDelicious neutron source models to reproduce the energy-angle distributions of the neutrons produced in IFMIF d-Li interactions.

Role of 18F-FDG PET/CT in Posttreatment Evaluation of Anal Carcinoma.

Science.gov (United States)

Houard, Clémence; Pinaquy, Jean-Baptiste; Mesguich, Charles; Henriques de Figueiredo, Bénédicte; Cazeau, Anne-Laure; Allard, Jean-Baptiste; Laharie, Hortense; Bordenave, Laurence; Fernandez, Philippe; Vendrely, Véronique

2017-09-01

The aim of this study was to evaluate the relevance of PET/CT and 18 F-FDG as a strategy for response evaluation after chemoradiotherapy for anal cancer. For this, the performance of posttreatment 18 F-FDG PET/CT, the impact on patient care, and the predictive value of metabolic response were assessed. Methods: This was a retrospective and multicenter analysis of 87 patients treated by chemoradiotherapy for anal squamous cell carcinoma between October 2007 and October 2013. All patients underwent systematic posttreatment 18 F-FDG PET/CT and were followed with at least a clinical examination every 4 mo for 2 y and every 6 mo thereafter. Disease progression was confirmed by biopsy for all patients in the case of local recurrence before surgery. Kaplan-Meier and Cox regression models were used to test for associations between metabolic or clinical endpoints and progression-free survival (PFS) or cause-specific survival (CSS). Results: The median follow-up was 25 mo. 18 F-FDG PET/CT was performed 1-8 mo (median, 4 mo) after completion of chemoradiotherapy. Overall, 25 patients relapsed and 13 died. The posttherapy 18 F-FDG PET/CT did not show any abnormal 18 F-FDG uptake (complete metabolic response [CMR]) in 55 patients whereas 32 displayed incomplete response (non-CMR): 15 patients with partial response and 17 with disease progression. The sensitivity of 18 F-FDG PET/CT to detect residual tumor tissue was 92% (95% confidence interval [CI], 75%-97%), specificity was 85% (95% CI, 75%-92%), positive predictive value was 72% (95% CI, 61%-90%), and negative predictive value was 96.4% (95% CI, 90%-98.7%). The 2-y PFS was 96% (95% CI, 90-100) for patients with CMR and 28% (95% CI, 14-47) for non-CMR patients ( P PET/CT changed patient management in 14 cases (16%), with relevant modifications in 12 (14%). A Cox proportional hazards model of survival outcome indicated that a CMR was the only significant predictor of PFS and CSS ( P PET/CT shows good accuracy in posttreatment

Search for high mass photon pairs in e +e - → f overlinefγγ ( f= e, μ, τ, v, q) at LEP

Science.gov (United States)

Buskulic, D.; de Bonis, I.; Decamp, D.; Ghez, P.; Goy, C.; Lees, J.-P.; Minard, M.-N.; Pietrzyk, B.; Alemany, R.; Ariztizabal, F.; Comas, P.; Crespo, J. M.; Delfino, M.; Fernandez, E.; Fernandez-Bosman, M.; Gaitan, V.; Garrido, Ll.; Mattison, T.; Pacheco, A.; Padilla, C.; Pascual, A.; Creanza, D.; de Palma, M.; Farilla, A.; Iaselli, G.; Maggi, G.; Natali, S.; Nuzzo, S.; Quattromini, M.; Ranieri, A.; Raso, G.; Romano, F.; Ruggieri, F.; Selvaggi, G.; Silvestris, L.; Tempesta, P.; Zito, G.; Chai, Y.; Hu, H.; Huang, D.; Huang, X.; Lin, J.; Wang, T.; Xie, Y.; Xu, D.; Xu, R.; Zhang, J.; Zhang, L.; Zhao, W.; Blucher, E.; Bonvicini, G.; Boudreau, J.; Casper, D.; Drevermann, H.; Forty, R. W.; Ganis, G.; Gay, C.; Hagelberg, R.; Harvey, J.; Haywood, S.; Hilgart, J.; Jacobsen, R.; Jost, B.; Knobloch, J.; Lehraus, I.; Lohse, T.; Maggi, M.; Markou, C.; Martinez, M.; Mato, P.; Meinhard, H.; Minten, A.; Miotto, A.; Miquel, R.; Moser, H.-G.; Palazzi, P.; Pater, J. R.; Perlas, J. A.; Pusztaszeri, J.-F.; Ranjard, F.; Redlinger, G.; Rolandi, L.; Rothberg, J.; Ruan, T.; Saich, M.; Schlatter, D.; Schmelling, M.; Sefkow, F.; Tejessy, W.; Veenhof, R.; Wachsmuth, H.; Wiedenmann, W.; Wildish, T.; Witzeling, W.; Wotschack, J.; Ajaltouni, Z.; Badaud, F.; Bardadin-Otwinowska, M.; El Fellous, R.; Falvard, A.; Gay, P.; Guicheney, C.; Henrard, P.; Jousset, J.; Michel, B.; Montret, J.-C.; Pallin, D.; Perret, P.; Podlyski, F.; Proriol, J.; Prulhière, F.; Saadi, F.; Fearnley, T.; Hansen, J. D.; Hansen, J. R.; Hansen, P. H.; Møllerud, R.; Nilsson, B. S.; Efthymiopoulos, I.; Kyriakis, A.; Simopoulou, E.; Vayaki, A.; Zachariadou, K.; Badier, J.; Blondel, A.; Bonneaud, G.; Brient, J. C.; Fouque, G.; Orteu, S.; Rougé, A.; Rumpf, M.; Tanaka, R.; Verderi, M.; Videau, H.; Candlin, D. J.; Parsons, M. I.; Veitch, E.; Focardi, E.; Moneta, L.; Parrini, G.; Corden, M.; Georgiopoulos, C.; Ikeda, M.; Lannutti, J.; Levinthal, D.; Sawyer, L.; Wasserbaech, S.; Antonelli, A.; Baldini, R.; Bencivenni, G.; Bologna, G.; Bossi, F.; Campana, P.; Capon, G.; Cerutti, F.; Chiarella, V.; D'Ettorre-Piazzoli, B.; Felici, G.; Laurelli, P.; Mannocchi, G.; Murtas, F.; Murtas, G. P.; Passalacqua, L.; Pepe-Altarelli, M.; Picchi, P.; Colrain, P.; Ten Have, I.; Lynch, J. G.; Maitland, W.; Morton, W. T.; Raine, C.; Reeves, P.; Scarr, J. M.; Smith, K.; Smith, M. G.; Thompson, A. S.; Turnbull, R. M.; Brandl, B.; Braun, O.; Geweniger, C.; Hanke, P.; Hepp, V.; Kluge, E. E.; Maumary, Y.; Putzer, A.; Rensch, B.; Stahl, A.; Tittel, K.; Wunsch, M.; Beuselinck, R.; Binnie, D. M.; Cameron, W.; Cattaneo, M.; Colling, D. J.; Dornan, P. J.; Greene, A. M.; Hassard, J. F.; Lieske, N. M.; Moutoussi, A.; Nash, J.; Patton, S.; Payne, D. G.; Phillips, M. J.; San Martin, G.; Sedgbeer, J. K.; Tomalin, I. R.; Wright, A. G.; Girtler, P.; Kneringer, E.; Kuhn, D.; Rudolph, G.; Bowdery, C. K.; Brodbeck, T. J.; Finch, A. J.; Foster, F.; Hughes, G.; Jackson, D.; Keemer, N. R.; Nuttall, M.; Patel, A.; Sloan, T.; Snow, S. W.; Whelan, E. P.; Kleinknecht, K.; Raab, J.; Renk, B.; Sander, H.-G.; Schmidt, H.; Steeg, F.; Walther, S. M.; Wanke, R.; Wolf, B.; Bencheikh, A. M.; Benchouk, C.; Bonissent, A.; Carr, J.; Coyle, P.; Drinkard, J.; Etienne, F.; Nicod, D.; Paplexiou, S.; Payre, P.; Roos, L.; Rousseau, D.; Schwemling, P.; Talby, M.; Adlung, S.; Assmann, R.; Bauer, C.; Blum, W.; Brown, D.; Cattaneo, P.; Dehning, B.; Dietl, H.; Dydak, F.; Frank, M.; Halley, A. W.; Jacobs, K.; Lauber, J.; Lütjens, G.; Lutz, G.; Männer, W.; Richter, R.; Schröder, J.; Schwarz, A. S.; Settles, R.; Seywerd, H.; Stierlin, U.; Stiegler, U.; St. Denis, R.; Wolf, G.; Boucrot, J.; Callot, O.; Cordier, A.; Davier, M.; Duflot, L.; Grivaz, J.-F.; Heusse, Ph.; Jaffe, D. E.; Janot, P.; Kim, D. W.; Le Diberder, F.; Lefrançois, J.; Lutz, A.-M.; Schune, M.-H.; Veillet, J.-J.; Videau, I.; Zhang, Z.; Abbaneo, D.; Bagliesi, G.; Batignani, G.; Bottigli, U.; Bozzi, C.; Calderini, G.; Carpinelli, M.; Ciocci, M. A.; Dell'Orso, R.; Ferrante, I.; Fidecaro, F.; Foà, L.; Forti, F.; Giassi, A.; Giorgi, M. A.; Gregorio, A.; Ligabue, F.; Lusiani, A.; Mannelli, E. B.; Marrocchesi, P. S.; Messineo, A.; Palla, F.; Rizzo, G.; Sanguinetti, G.; Spagnolo, P.; Steinberger, J.; Tenchini, R.; Tonelli, G.; Triggiani, G.; Vannini, C.; Venturi, A.; Verdini, P. G.; Walsh, J.; Betteridge, A. P.; Carter, J. M.; Gao, Y.; Green, M. G.; March, P. V.; Mir, Ll. M.; Medcalf, T.; Quazi, I. S.; Strong, J. A.; West, L. R.; Botterill, D. R.; Clifft, R. W.; Edgecock, T. R.; Edwards, M.; Fisher, S. M.; Jones, T. J.; Norton, P. R.; Salmon, D. P.; Thompson, J. C.; Bloch-Devaux, B.; Colas, P.; Duarte, H.; Emery, S.; Kozanecki, W.; Lançon, E.; Lemaire, M. C.; Locci, E.; Marx, B.; Perez, P.; Rander, J.; Renardy, J.-F.; Rosowsky, A.; Roussarie, A.; Schuller, J.-P.; Schwindling, J.; Si Mohand, D.; Vallage, B.; Johnson, R. P.; Litke, A. M.; Taylor, G.; Wear, J.; Ashman, J. G.; Babbage, W.; Booth, C. N.; Buttar, C.; Cartwright, S.; Combley, F.; Dawson, I.; Thompson, L. F.; Barberio, E.; Böhrer, A.; Brandt, S.; Cowan, G.; Grupen, C.; Lutters, G.; Rivera, F.; Schäfer, U.; Smolik, L.; Bosisio, L.; Della Marina, R.; Giannini, G.; Gobbo, B.; Ragusa, F.; Bellantoni, L.; Chen, W.; Cinabro, D.; Conway, J. S.; Cowen, D. F.; Ferguson, D. P. S.; Gao, Y. S.; Grahl, J.; Harton, J. L.; Jared, R. C.; Leclaire, B. W.; Lishka, C.; Pan, Y. B.; Saadi, Y.; Schmitt, M.; Sharma, V.; Shi, Z. H.; Walsh, A. M.; Weber, F. V.; Wu, Sau Lan; Wu, X.; Zheng, M.; Zobernig, G.; Aleph Collaboration

1993-07-01

The result of a search for high mass photon pairs from the processes e +e - → f overlinefγγ (f = e, μ, τ, v and q) with the ALEPH detector is reported. The result for f = e, μ and τ is to be compared with the observation of 4 events by the L3 Collaboration with invariant masses, Mγγ, of the two photons near 60 GeV. From a data sample approximately twice as large taken from 1990 to 1992, 6 events are found with Mγγ distributed between 50 GeV and 72 GeV, while 4.9 events are expected from a QED calculation. There is no evidence for a mass peak; only one event ( μ+μ-γγ) at Mγγ = 59.4 ± 0.2 GeV is compatible with the L3 observation. In addition, for Mγγ > 50 GeV, no event is found for e +e - → q overlineqγγ and only one event is found consistent with e +e - → v overlinevγγ ; this event has Mγγ = 58.5 ± 1.9 GeV. High mass photon pair events have also been searched for in γγ collisions. This allows one to set an upper limit of 50 MeV for the width of an assumed resonance decaying to photon pairs.

Evaluation of 6-([18F] fluoroacetamido)-1-hexanoic-anilide (18F-FAHA) as imaging probe in tumor xenograft mice model

Science.gov (United States)

Li, Fiona; Cho, Sung Ju; Yu, Lihai; Hudson, Robert H. E.; Luyt, Leonard G.; Pin, Christopher L.; Kovacs, Michael S.; Koropatnick, James; Lee, Ting-Yim

2016-03-01

Alteration in genetic expression is as important as gene mutation in cancer development and proliferation. Epigenetic changes affect gene expression without altering the DNA sequence. Histone deacetylase (HDAC), an enzyme facilitating histone remodelling, can lead to silencing of tumor suppressor genes making HDAC inhibitors viable anticancer drugs against tumors with increased activity of the enzyme. In this study we evaluated 18F-fluroacetamido-1-hexanoicanilide (18F-FAHA), an artificial HDAC substrate, as imaging probe of HDAC activity of human tumor xenografts in immunocompromised host mice. Human breast and melanoma cell lines, MDA-MB-468 and MDA-MB-435 respectively, known to overexpress HDAC activity were xenografted into immunocompromised mice and HDAC activity was imaged using 18F-FAHA. The melanoma group was treated with saline, SAHA (suberoylanilide hydroxamic acid, an approved anticancer HDAC inhibitor) in DMSO, or DMSO as positive control. Tracer kinetic modelling and SUV were used to estimate HDAC activity from dynamic PET data. Both breast tumor and melanoma group showed great variability in binding rate constant (BRC) of 18F-FAHA suggesting highly variable inter- and intra-tumoral HDAC activity. For the SAHA treated melanoma group, HDAC activity, as monitored by BRC of 18F-FAHA, decreased more than the two (positive and negative) control groups but not tumor growth. Our preliminary study showed that noninvasive PET imaging with 18F-FAHA has the potential to identify patients for whom treatment with HDAC inhibitors are appropriate, to assess the effectiveness of that treatment as an early marker of target reduction, and also eliminate the need for invasive tissue biopsy to individualize treatment.

Design and evaluation of a campaign to promote the consumption of vegetables and fruits in Mexican school-age children.

Science.gov (United States)

Galván, Marcos; Ríos-Pérez, Fernanda; López-Rodríguez, Guadalupe; Guzmán-Saldaña, Rebeca; Fernández-Cortés, Trinidad Lorena; Camacho-Bernal, Gloria; Robles-Acevedo, Manuel

2016-09-20

In Mexican school-age children the consumption of vegetables and fruits (V&F) is less than 25% of the amount recommended by the World Health Organization. Evaluate the effectiveness of a promotion campaign about the consumption of V&F in urban school children from Hidalgo, Mexico. A non controlled community trial in 226 school-age children from private and public schools was conducted, we designed and distributed printed material for promoting the consumption of V&F among the school population, access to V&F was provided through planning and sales at school stores, and consumption was supervised by the teachers. At the end of the intervention vegetable consumption increased by 50 g and plain water in 100 mL (T-test, p < 0.05); the proportion of school-age children who identified the health benefits of the consumption of V&F increased significantly (68% initial, 87% final) and greater support from parents (61% initial, 92 final%) was achieved. A promotion campaign and improved access to vegetables, fruits and water in the school environment which is supported by parents and teachers can encourage healthier eating at school.

Production of muon pairs with masses greater than 4 GeV/c2 in anti p N and π-N interactions at 125 GeV/c

International Nuclear Information System (INIS)

Anassontzis, E.; Katsanevas, S.; Kostarakis, P.

1982-07-01

We have measured the high mass (M > 4 GeV/c 2 ) dimuons produced in anti-proton-nucleon and pi minus-nucleon interactions. Preliminary differential cross sections are presented as a function of pair mass, x/suf F/, p/sub T/, and √ tau. Comparisons of these cross sections with the predictions of the Drell-Yan model are discussed and preliminary values for the K factor for the anti p and π - induced reactions are reported

TU-F-18A-01: Preliminary Results of a Prototype Quality Control Process for Spectral CT

International Nuclear Information System (INIS)

Nute, J; Jacobsen, M; Pennington, J; Cody, D; Chandler, A; Imai, Y; Baiu, C

2014-01-01

Purpose: A prototype quality control (QC) phantom and analysis process has been designed specifically to monitor dual-energy CT and address the current lack of quantitative oversight of the spectral capabilities of these scanners. Methods: A prototype solid water phantom was designed with multiple material inserts, and to support both head and body protocols. Inserts included tissue equivalent and material rods (iodine, iron, calcium) at various concentrations. The oval body phantom, measuring 30cm×40cm×15cm, was scanned using four dual-energy protocols with CTDIvol ranges of 19.6–62mGy (0.516 pitch) and 10.3–32.5mGy (0.984 pitch), and rotation times ranging from 0.5-1sec. The circular head phantom, measuring 22cm in diameter by 15cm, was scanned using three dual-energy protocols with CTDIvol ranges of 67–132.6mGy (0.531 pitch) and 36.7–72.7mGy (0.969 pitch), and rotation times ranging from 0.5–0.9sec. All images were reconstructed at 50, 70, 110 and 140 keV, and using a water-iodine material basis pair. The images were evaluated for iodine quantification accuracy and stability of monoenergetic reconstructions. The phantom was scanned twice on ten GE 750HD CT scanners to evaluate inter-scanner agreement, as well as ten times on a single scanner over a oneweek period to evaluate intra-scanner repeatability. Results: Preliminary analysis revealed consistent (inter- and intra-scanner) iodine quantification accuracy within 10% was only achieved for protocols in the upper half of dose levels assessed when grouped by pitch. Although all scanners undergo rigorous daily single-energy QC, iodine quantification accuracy from one scanner unexpectedly deviated from the other nine substantially. In general, inter-scanner agreement and intra-scanner repeatability varied with dose, rotation time and reconstructed keV. Conclusion: Preliminary results indicate the need for a dual-energy QC process to ensure inter-scanner agreement and intra-scanner repeatability. In

EFSA Scientific Opinion on Flavouring Group Evaluation 87 Revision 1 (FGE.87Rev1): Consideration of bicyclic secondary alcohols, ketones and related esters evaluated by JECFA (63rd meeting) structurally related to bicyclic secondary alcohols, ketones and related esters evaluated by EFSA in FGE.47

DEFF Research Database (Denmark)

Larsen, John Christian; Nørby, Karin Kristiane; Beltoft, Vibe Meister

evaluation is necessary, as laid down in Commission Regulation (EC) No 1565/2000. The present consideration concerns a group of 17 bicyclic secondary alcohols, ketones and related esters evaluated by the JECFA at the 63rd meeting in 2004. This revision of FGE.87 is made due to consideration of two additional...

Interfacial reaction in SiC_f/Ti-6Al-4V composite by using transmission electron microscopy

International Nuclear Information System (INIS)

Huang, Bin; Li, Maohua; Chen, Yanxia; Luo, Xian; Yang, Yanqing

2015-01-01

The interfacial reactions of continuous SiC fiber reinforced Ti-6Al-4V matrix composite (SiC_f/Ti-6Al-4V composite) and continuous SiC fiber coated by C reinforced Ti-6Al-4V matrix composite (SiC_f/C/Ti-6Al-4V composite) were investigated by using micro-beam electron diffraction (MBED) and energy disperse spectroscopy (EDS) on transmission electron microscopy (TEM). The sequence of the interfacial reactions in the as-processed and exposed at 900°C for 50h SiC_f/Ti-6Al-4V composites can be described as SiC||TiC||Ti_5Si_3 + TiC||Ti-6Al-4V and SiC||TiC||Ti_5Si_3||TiC||Ti_5Si_3||TiC||Ti_5Si_3||Ti-6Al-4V, respectively. Additionally, both in as-processed and exposed composites, Ti_3SiC_2 and Ti_3Si are absent at the interfaces. For the SiC_f/C/Ti-6Al-4V composite exposed at 900 °C for 50 h, the sequence of the interfacial reaction can be described as SiC||C||TiC_F||TiC_C||Ti-6Al-4V before C coating is completely consumed by interfacial reaction. When interfacial reaction consumes C coating completely, the sequence of the interfacial reaction can be described as SiC||TiC||Ti_5Si_3||TiC||Ti-6Al-4V. Furthermore, in SiC_f/C/Ti-6Al-4V composite, C coating can absolutely prevent Si diffusion from SiC fiber to matrix. Basing on these results, the model of formation process of the interfacial reaction products in the composites was proposed. - Highlights: • We obtained the sequence of the interfacial reactions in the as-processed and exposed at 900 °C for 50 h SiC_f/Ti-6Al-4 V composites as well as in the SiC_f/C/Ti-6Al-4 V composite exposed at 900 °C for 50 h. • We verified that both in as-processed and exposed SiC_f/Ti-6Al-4 V composites, Ti_3SiC_2 and Ti_3Si are absent at the interfaces. • Carbon coating can absolutely prevent silicon diffusion from SiC fiber to matrix. • Basing on these results, the model of formation process of the interfacial reaction products in the composites was proposed.

Theoretical estimation of "6"4Cu production with neutrons emitted during "1"8F production with a 30 MeV medical cyclotron

International Nuclear Information System (INIS)

Auditore, Lucrezia; Amato, Ernesto; Baldari, Sergio

2017-01-01

Purpose: This work presents the theoretical estimation of a combined production of "1"8F and "6"4Cu isotopes for PET applications. "6"4Cu production is induced in a secondary target by neutrons emitted during a routine "1"8F production with a 30 MeV cyclotron: protons are used to produce "1"8F by means of the "1"8O(p,n)"1"8F reaction on a ["1"8O]-H_2O target (primary target) and the emitted neutrons are used to produce "6"4Cu by means of the "6"4Zn(n,p)"6"4Cu reaction on enriched zinc target (secondary target). Methods: Monte Carlo simulations were carried out using Monte Carlo N Particle eXtended (MCNPX) code to evaluate flux and energy spectra of neutrons produced in the primary (Be+["1"8O]-H_2O) target by protons and the attenuation of neutron flux in the secondary target. "6"4Cu yield was estimated using an analytical approach based on both TENDL-2015 data library and experimental data selected from EXFOR database. Results: Theoretical evaluations indicate that about 3.8 MBq/μA of "6"4Cu can be obtained as a secondary, ‘side’ production with a 30 MeV cyclotron, for 2 h of irradiation of a proper designed zinc target. Irradiating for 2 h with a proton current of 120 μA, a yield of about 457 MBq is expected. Moreover, the most relevant contaminants result to be "6"3","6"5Zn, which can be chemically separated from "6"4Cu contrarily to what happens with proton irradiation of an enriched "6"4Ni target, which provides "6"4Cu mixed to other copper isotopes as contaminants. Conclusions: The theoretical study discussed in this paper evaluates the potential of the combined production of "1"8F and "6"4Cu for medical purposes, irradiating a properly designed target with 30 MeV protons. Interesting yields of "6"4Cu are obtainable and the estimation of contaminants in the irradiated zinc target is discussed. - Highlights: • "6"4Cu production with secondary neutrons from "1"8F production with protons was investigated. • Neutron reactions induced in enriched "6"4Zn

A technique to consider mismatches between fMRI and EEG/MEG sources for fMRI-constrained EEG/MEG source imaging: a preliminary simulation study

International Nuclear Information System (INIS)

Im, Chang-Hwan; Lee, Soo Yeol

2006-01-01

fMRI-constrained EEG/MEG source imaging can be a powerful tool in studying human brain functions with enhanced spatial and temporal resolutions. Recent studies on the combination of fMRI and EEG/MEG have suggested that fMRI prior information could be readily implemented by simply imposing different weighting factors to cortical sources overlapping with the fMRI activations. It has been also reported, however, that such a hard constraint may cause severe distortions or elimination of meaningful EEG/MEG sources when there are distinct mismatches between the fMRI activations and the EEG/MEG sources. If one wants to obtain the actual EEG/MEG source locations and uses the fMRI prior information as just an auxiliary tool to enhance focality of the distributed EEG/MEG sources, it is reasonable to weaken the strength of fMRI constraint when severe mismatches between fMRI and EEG/MEG sources are observed. The present study suggests an efficient technique to automatically adjust the strength of fMRI constraint according to the mismatch level. The use of the proposed technique rarely affects the results of conventional fMRI-constrained EEG/MEG source imaging if no major mismatch between the two modalities is detected; while the new results become similar to those of typical EEG/MEG source imaging without fMRI constraint if the mismatch level is significant. A preliminary simulation study using realistic EEG signals demonstrated that the proposed technique can be a promising tool to selectively apply fMRI prior information to EEG/MEG source imaging

Preliminary Monthly Climatological Summaries

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Preliminary Local Climatological Data, recorded since 1970 on Weather Burean Form 1030 and then National Weather Service Form F-6. The preliminary climate data pages...

Adrenal incidentaloma and the Janus Kinase 2 V617F mutation: A case-based review of the literature

Directory of Open Access Journals (Sweden)

Mustafa Unubol

2013-01-01

Full Text Available Adrenal incidentaloma was detected in an 81-year-old male patient and a 37-year-old female patient who had been diagnosed with essential thrombocytosis. Each patient′s Janus Kinase 2 (JAK2 V617F mutation was positive, and they were evaluated as having non-functional adrenal incidentaloma. The JAK2 activates the signal transducers and activators of transcription (STAT proteins which then activate the phosphoinositol-3 kinases, Ras, mitogen-activated protein (MAP kinases, and transcription. Constitutive activation causes cell proliferation and dysregulation of apoptosis. It is thought that STAT3 activation-mediated JAK family kinases have a central role in the solid tumor cell series. Permanent activation of STAT3 and STAT5 causes tumor cell proliferation, survival, metastasis, and an increase in tumor-mediated inflammation in solid and hematologic tumors. According to our literature screening, irregular JAK signaling, seen at the pathogenesis of many solid and hematologic tumors, has not been previously evaluated with regard to adrenal tumors. As a result, our cases are the first coexistence of JAK V617F mutation with adrenal incidentaloma in the literature. Because of this, we think that JAK2 mutation must be evaluated to clarify the etiology of adrenal incidentalomas.

Comparative study on the degradation of dibutyl phthalate by two newly isolated Pseudomonas sp. V21b and Comamonas sp. 51F

Directory of Open Access Journals (Sweden)

Vinay Kumar

2017-09-01

Full Text Available Dibutyl phthalate is (DBP the top priority toxicant responsible for carcinogenicity, teratogenicity and endocrine disruption. This study demonstrates the DBP degradation capability of the two newly isolated bacteria from municipal solid waste leachate samples. The isolated bacteria were designated as Pseudomonas sp. V21b and Comamonas sp. 51F after scanning electron microscopy, transmission electron microscopy, Gram-staining, antibiotic sensitivity tests, biochemical characterization, 16S-rRNA gene identification and phylogenetic studies. They were able to grow on DBP, benzyl butyl phthalate, monobutyl phthalate, diisodecyl phthalate, dioctyl phthalate, and protocatechuate. It was observed that Pseudomonas sp. V21b was more efficient in DBP degradation when compared with Comamonas sp. 51F. It degraded 57% and 76% of the initial DBP in minimal salt medium and in DBP contaminated samples respectively. Kinetics for the effects of DBP concentration on Pseudomonas sp. V21b and Comamonas sp. 51F growth was also evaluated. Stoichiometry for DBP degradation and biomass formation were compared for both the isolates. Two major metabolites diethyl phthalate and monobutyl phthalates were identified using GC–MS in the extracts. Key genes were amplified from the genomes of Pseudomonas sp. V21b and Comamonas sp. 51F. DBP degradation pathway was also proposed.

Calculation of the hyperfine constants of the V sub (K) center in CaF2, SrF2 e BaF2

International Nuclear Information System (INIS)

Bufaical, R.F.

1975-03-01

The magnetic hyperfine constants of the V sub(K) center in CaF 2 , SrF 2 and BaF 2 have been calculated, assuming a phenomenological model, based on the F - 2 'central molecule', to describe the wave function of the defect. The introduction of covalence with the ions neighboring the 'central molecule', has shown that this is a better description for the defect than a simple 'central molecule' model. It was also shown that the results for the hyperfine constants are strongly dependent on the relaxations of these neighboring ions, which have been determined by fitting the experimental data. The present results are compared with other previous calculations where similar and different methods have been used. A better description for the wave function of the defect is suggested. (author) [pt

Utility of F-18 FDG PET/CT on the evaluation of primary bone lymphoma.

Science.gov (United States)

Wang, Li-Juan; Wu, Hu-Bing; Wang, Meng; Han, Yan-Jiang; Li, Hong-Sheng; Zhou, Wen-Lan; Wang, Quan-Shi

2015-11-01

Primary bone lymphoma (PBL) is a rare type of malignant lymphoma. Few data have been reported regarding the utility of F-18 FDG PET/CT in this disease. The aim of this study was to assess the role of F-18 FDG PET/CT in the diagnosis and therapeutic effect evaluation of PBL. A total of 19 consecutive patients with PBL were enrolled. Whole-body PET/CT scan was performed for all patients. The diagnosis of PBL was established by histopathology and immunohistochemistry. F-18 FDG PET/CT was positive in 94.7% (18/19) of patients. Uptake of FDG in lesions was intense with SUVmax of 15.14 ± 11.82. Multiple involved lesions were found in 47.4% (9/19) patients, while 52.6% presented with a single involved lesion. Based on the lesions, PET detected 98.9% (87/88) lesions. Among them, 71.6% (63/88) lesions were found to be located in axial skeleton and 28.4% (25/88) in the extremity skeleton. FDG PET/CT also found the lesions infiltrate to the surrounding soft tissue in 84.2% (16/19) patients. On the syn-modality CT, the bone destruction was noted in 43.2% (38/88) of the lesions, of which 50.0% lesions presented as slight change in bone density and 50.0% as severe change. The diagnostic sensitivity of PET was much higher than that of CT (98.9% vs. 43.2%, P=0.000). PET/CT was performed for evaluation of treatment response in 13 patients. In 12 patients with complete response(CR), PET/CT found the 25 lesions were F-18 FDG fully resoluted after treatment, however, bone destruction was still presented in 72.0% (18/25) lesions. The present study suggests that F-18 FDG PET/CT was a sensitive imaging modality for diagnosis and treatment response evaluation of PBL. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Comparative Evaluation of Spectral Transmittance of Some Welding Glasses with ANSI Z87.1

Directory of Open Access Journals (Sweden)

saeed Rahmani

2016-04-01

Full Text Available Background: Welding emits harmful rays to the eyes. We evaluate and compare the UV, blue light and IR transmittance characteristics of some currently available welding safety protectors with ANSI Z87.1 criteria.Materials and Methods: Three type of welding safety protectors (three of each type have been evaluated for spectral transmittance. One-sample T-test was performed to establish is there a statistically significant difference between the standard criteria and UV, blue and IR regions for the welding protectors (α=0.05.Results: In ultraviolet (UV (far and near region, two types of the tested protectors (P1, P2 transmitted lower than the maximum allowable value specified in the standard (P<0.001. In infrared (IR spectrum only one type (P3 transmitted lower than the maximum allowable value specified in the standard (P<0.001. In blue light region, all of the tested protectors transmitted lower than the maximum allowable value specified in the standard (P<0.001.Conclusion: Although all of the tested glasses could meet some parts of the criteria, but none of them could meet the complete spectral transmittance requirements of ANSI Z87.1.

Mutagenesis in yam, Discorea rotundata: Clonal evaluation of M1V3 yam plants

International Nuclear Information System (INIS)

Nwachukwu, E.C.; Mbanso, E.N.; Ene, L.S.O.

1997-01-01

Ten thousand plants of M 1 V 3 population of the white guinea yam, Dioscorea rotundata Poir, were evaluated. There was no consistent trends in variations in plant height, number of branches, branching heights and number of leaves of the treated tubers. However, plant height were lower in the irradiated than in the control. The coefficients of variations (C.V.) were higher in the irradiated than in the control populations, indicating wider variations in the former population. Based on yield performance, 110 tubers were selected for preliminary yield trials, Considering that diplontic selection may occur in the irradiated micro-tubers, the experiment will also be carried out using nodal cultures in vitro. (author). 2 tabs

Mutated and Bacteriophage T4 Nanoparticle Arrayed F1-V Immunogens from Yersinia pestis as Next Generation Plague Vaccines

Science.gov (United States)

Tao, Pan; Mahalingam, Marthandan; Kirtley, Michelle L.; van Lier, Christina J.; Sha, Jian; Yeager, Linsey A.; Chopra, Ashok K.; Rao, Venigalla B.

2013-01-01

Pneumonic plague is a highly virulent infectious disease with 100% mortality rate, and its causative organism Yersinia pestis poses a serious threat for deliberate use as a bioterror agent. Currently, there is no FDA approved vaccine against plague. The polymeric bacterial capsular protein F1, a key component of the currently tested bivalent subunit vaccine consisting, in addition, of low calcium response V antigen, has high propensity to aggregate, thus affecting its purification and vaccine efficacy. We used two basic approaches, structure-based immunogen design and phage T4 nanoparticle delivery, to construct new plague vaccines that provided complete protection against pneumonic plague. The NH2-terminal β-strand of F1 was transplanted to the COOH-terminus and the sequence flanking the β-strand was duplicated to eliminate polymerization but to retain the T cell epitopes. The mutated F1 was fused to the V antigen, a key virulence factor that forms the tip of the type three secretion system (T3SS). The F1mut-V protein showed a dramatic switch in solubility, producing a completely soluble monomer. The F1mut-V was then arrayed on phage T4 nanoparticle via the small outer capsid protein, Soc. The F1mut-V monomer was robustly immunogenic and the T4-decorated F1mut-V without any adjuvant induced balanced TH1 and TH2 responses in mice. Inclusion of an oligomerization-deficient YscF, another component of the T3SS, showed a slight enhancement in the potency of F1-V vaccine, while deletion of the putative immunomodulatory sequence of the V antigen did not improve the vaccine efficacy. Both the soluble (purified F1mut-V mixed with alhydrogel) and T4 decorated F1mut-V (no adjuvant) provided 100% protection to mice and rats against pneumonic plague evoked by high doses of Y. pestis CO92. These novel platforms might lead to efficacious and easily manufacturable next generation plague vaccines. PMID:23853602

Mutated and bacteriophage T4 nanoparticle arrayed F1-V immunogens from Yersinia pestis as next generation plague vaccines.

Directory of Open Access Journals (Sweden)

Pan Tao

Full Text Available Pneumonic plague is a highly virulent infectious disease with 100% mortality rate, and its causative organism Yersinia pestis poses a serious threat for deliberate use as a bioterror agent. Currently, there is no FDA approved vaccine against plague. The polymeric bacterial capsular protein F1, a key component of the currently tested bivalent subunit vaccine consisting, in addition, of low calcium response V antigen, has high propensity to aggregate, thus affecting its purification and vaccine efficacy. We used two basic approaches, structure-based immunogen design and phage T4 nanoparticle delivery, to construct new plague vaccines that provided complete protection against pneumonic plague. The NH₂-terminal β-strand of F1 was transplanted to the COOH-terminus and the sequence flanking the β-strand was duplicated to eliminate polymerization but to retain the T cell epitopes. The mutated F1 was fused to the V antigen, a key virulence factor that forms the tip of the type three secretion system (T3SS. The F1mut-V protein showed a dramatic switch in solubility, producing a completely soluble monomer. The F1mut-V was then arrayed on phage T4 nanoparticle via the small outer capsid protein, Soc. The F1mut-V monomer was robustly immunogenic and the T4-decorated F1mut-V without any adjuvant induced balanced TH1 and TH2 responses in mice. Inclusion of an oligomerization-deficient YscF, another component of the T3SS, showed a slight enhancement in the potency of F1-V vaccine, while deletion of the putative immunomodulatory sequence of the V antigen did not improve the vaccine efficacy. Both the soluble (purified F1mut-V mixed with alhydrogel and T4 decorated F1mut-V (no adjuvant provided 100% protection to mice and rats against pneumonic plague evoked by high doses of Y. pestis CO92. These novel platforms might lead to efficacious and easily manufacturable next generation plague vaccines.

Development of an HPLC method for the radiochemical purity evaluation of [{sup 18}F]Fluoroestradiol

Energy Technology Data Exchange (ETDEWEB)

Bispo, Ana Carolina de A.; Nascimento, Leonardo T.C. do; Costa, Flávia M.; Silva, Juliana B. da, E-mail: anacarollbispo@gmail.com [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Unidade de Pesquisa e Produção de Radiofármacos; Mamede, Marcelo, E-mail: mamede.mm@gmail.com [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Departamento de Anatomia e Imagem

2017-07-01

{sup 18}F-Fluoroestradiol ([{sup 18}F]FES), an estrogen analog, is a radiopharmaceutical used in Positron Emission Tomography (PET) that allows evaluating the tumor cell receptor profile and the best therapy strategy, the staging, the prognosis and the response to therapy in several breast cancer cases. As there is not any pharmacopoeia's monograph of [{sup 18}F]FES to standardize its quality control criteria, this work presents a new HPCL's method to perform the [{sup 18}F]FES radiochemical purity. A liquid chromatograph was used with radioactivity and ultraviolet detectors. Three concentrations of fluoroestradiol standard solution were used along the test. Their retention time was compared to its relative radiolabelled analogue to confirm its identity. Several mobile phases with acetonitrile and two mobile phase flows were tested to optimize the runs. Peaks symmetry, retention time, theoretical plates and resolution were analyzed to choose the best conditions. The mean retention time of both standard Fluoroestradiol and [{sup 18}F]FES solutions were the same, demonstrating that [{sup 18}F]FES formulation did not interfere with [{sup 18}F]FES analysis. The best conditions were 1.2 mL/min and isocratic 40% V/V acetonitrile in water, which gave [{sup 18}F]FES peak resolution greater than 6 and symmetry factor of 1. Thus, the developed method is ready to be validated and implemented in [{sup 18}F]FES quality control routine in CDTN/Brazil. (author)

Pharmacological characterization of canine melancortin-4 receptor and its natural variant V213F.

Science.gov (United States)

Yan, J; Tao, Y-X

2011-08-01

Dogs have become one of the most important companion animals in modern society. However, it is estimated that 20% to 40% of owned dogs are obese, suggesting that obesity has become one of the most important canine health problem. In addition, obesity in dogs also leads to type II diabetes. Because the melanocortin-4 receptor (MC4R) has been shown to be essential in maintaining energy homeostasis in several different species, including rodents and humans, we initiated studies toward elucidating the roles of MC4R in obesity pathogenesis in dogs. Canine MC4R has been cloned, and a missense variant V213F was identified. We designed primers and successfully cloned canine MC4R and generated the variant V213F by site-directed mutagenesis. The objective of this study was to investigate the pharmacological properties of canine MC4R and its natural variant V213F. We measured ligand binding and signaling properties with the use of both natural and synthetic ligands. Human MC4R was also included in the experiments for comparison. Both wild-type canine MC4R and its natural variant V213F functioned normally in terms of binding and signaling. Of the ligands we used, [Nle(4), D-Phe(7)]-α-melanocyte-stimulating hormone is the most potent ligand. We conclude that the cloned canine MC4R is a functional receptor, and the natural variant V213F does not have any functional defect and therefore is not likely to cause obesity in dogs. Copyright © 2011 Elsevier Inc. All rights reserved.

The association of JAK2V617F mutation and leukocytosis with thrombotic events in essential thrombocythemia.

Science.gov (United States)

Hsiao, Hui-Hua; Yang, Ming-Yu; Liu, Yi-Chang; Lee, Ching-Ping; Yang, Wen-Chi; Liu, Ta-Chih; Chang, Chao-Sung; Lin, Sheng-Fung

2007-11-01

The Janus kinase 2 mutation, JAK2 (V617F), and megakaryocytic mutations, MPL (W515L/K), have been identified and correlated with a subtype of essential thrombocythemia (ET) patients. We investigated the frequency of mutations in ET patients and analyzed the relationship with their clinical features. Fifty-three ET patients were enrolled in the study. The amplification refractory mutation system was applied for the mutation survey of the JAK2V617F, while the polymerase chain reaction with sequencing was used for the mutation survey of MPLW515L/K. Thirty-five (66%) patients harboring the JAK2 (V617F) mutation, including 3 homozygous and 32 heterozygous changes, but no MPLW515L/K mutation, were found. During follow-up, 17 (32.1%) patients suffered from documented thrombotic events, with 15 having JAK2V617F mutations. Statistical analysis showed that patients with the JAK2 mutation had significantly higher leukocytes, hemoglobin level, and thrombotic event (p = 0.043, p = 0.001, and p = 0.029, respectively). Thrombotic events were also significantly correlated with leukocytosis and older age. The JAK2V617F mutation was noted in a certain population of ET patients and correlated with leukocytosis, high hemoglobin level, and thrombosis. Therefore, detection of the JAK2V617F mutation can affect not only the diagnosis, but also the management of ET patients.

Measurements of the {sup 235}U(n,f) cross section in the 3 to 30 MeV neutron energy region

Energy Technology Data Exchange (ETDEWEB)

Carlson, A.D.; Wasson, O.A. [National Institute of Standards and Technology, Gaithersburg, MD (United States); Lisowski, P.W. [Los Alamos National Lab., NM (United States)] [and others

1991-12-31

To improve the accuracy of the {sup 235}U(n,f) cross section, measurements have been made of this standard cross section at the target 4 facility at Los Alamos National Laboratory (LANL). The data were obtained at the 20-meter flight path of that facility. The fission reaction rate was determined with a fast parallel plate ionization chamber and the neutron fluence was measured with an annular proton recoil telescope. The measurements provide the shape of the {sup 235}U(n,f) cross section relative to the hydrogen scattering cross section for neutron energies from about 3 to 30 MeV neutron energy. The data have been normalized to the very accurately known value near 14 MeV. The results are in good agreement with the ENDF/B-VI evaluation up to about 15 MeV neutron energy. Above this energy differences as large as 5% are observed.

Evaluation of alternative chemical additives for high-level waste vitrification feed preparation processing

International Nuclear Information System (INIS)

Seymour, R.G.

1995-01-01

During the development of the feed processing flowsheet for the Defense Waste Processing Facility (DWPF) at the Savannah River Site (SRS), research had shown that use of formic acid (HCOOH) could accomplish several processing objectives with one chemical addition. These objectives included the decomposition of tetraphenylborate, chemical reduction of mercury, production of acceptable rheological properties in the feed slurry, and controlling the oxidation state of the glass melt pool. However, the DEPF research had not shown that some vitrification slurry feeds had a tendency to evolve hydrogen (H 2 ) and ammonia (NH 3 ) as the result of catalytic decomposition of CHOOH with noble metals (rhodium, ruthenium, palladium) in the feed. Testing conducted at Pacific Northwest Laboratory and later at the Savannah River Technical Center showed that the H 2 and NH 3 could evolve at appreciable rates and quantities. The explosive nature of H 2 and NH 3 (as ammonium nitrate) warranted significant mitigation control and redesign of both facilities. At the time the explosive gas evolution was discovered, the DWPF was already under construction and an immediate hardware fix in tandem with flowsheet changes was necessary. However, the Hanford Waste Vitrification Plant (HWVP) was in the design phase and could afford to take time to investigate flowsheet manipulations that could solve the problem, rather than a hardware fix. Thus, the HWVP began to investigate alternatives to using HCOOH in the vitrification process. This document describes the selection, evaluation criteria, and strategy used to evaluate the performance of the alternative chemical additives to CHOOH. The status of the evaluation is also discussed

New sensorless, efficient optimized and stabilized v/f control for pmsm machines

Science.gov (United States)

Jafari, Seyed Hesam

With the rapid advances in power electronics and motor drive technologies in recent decades, permanent magnet synchronous machines (PMSM) have found extensive applications in a variety of industrial systems due to its many desirable features such as high power density, high efficiency, and high torque to current ratio, low noise, and robustness. In low dynamic applications like pumps, fans and compressors where the motor speed is nearly constant, usage of a simple control algorithm that can be implemented with least number of the costly external hardware can be highly desirable for industry. In recent published works, for low power PMSMs, a new sensorless volts-per-hertz (V/f) controlling method has been proposed which can be used for PMSM drive applications where the motor speed is constant. Moreover, to minimize the cost of motor implementation, the expensive rotor damper winding was eliminated. By removing the damper winding, however, instability problems normally occur inside of the motor which in some cases can be harmful for a PMSM drive. As a result, to address the instability issue, a stabilizing loop was developed and added to the conventional V/f. By further studying the proposed sensorless stabilized V/f, and calculating power loss, it became known that overall motor efficiency still is needed to be improved and optimized. This thesis suggests a new V/f control method for PMSMs, where both efficiency and stability problems are addressed. Also, although in nearly all recent related research, methods have been applied to low power PMSM, for the first time, in this thesis, the suggested method is implemented for a medium power 15 kW PMSM. A C2000 F2833x Digital Signal Processor (DSP) is used as controller part for the student custom built PMSM drive, but instead of programming the DSP in Assembly or C, the main control algorithm was developed in a rapid prototype software environment which here Matlab Simulink embedded code library is used.

Critique of Hanford Waste Vitrification Plant off-gas sampling requirements

International Nuclear Information System (INIS)

Goles, R.W.

1996-03-01

Off-gas sampling and monitoring activities needed to support operations safety, process control, waste form qualification, and environmental protection requirements of the Hanford Waste Vitrification Plant (HWVP) have been evaluated. The locations of necessary sampling sites have been identified on the basis of plant requirements, and the applicability of Defense Waste Processing Facility (DWPF) reference sampling equipment to these HWVP requirements has been assessed for all sampling sites. Equipment deficiencies, if present, have been described and the bases for modifications and/or alternative approaches have been developed

FERMI LARGE AREA TELESCOPE OBSERVATIONS OF THE SUPERNOVA REMNANT G8.7–0.1

International Nuclear Information System (INIS)

Ajello, M.; Allafort, A.; Bechtol, K.; Berenji, B.; Blandford, R. D.; Bloom, E. D.; Borgland, A. W.; Buehler, R.; Cameron, R. A.; Baldini, L.; Bellazzini, R.; Bregeon, J.; Ballet, J.; Barbiellini, G.; Bastieri, D.; Buson, S.; Bonamente, E.; Brigida, M.; Bruel, P.; Caliandro, G. A.

2012-01-01

We present a detailed analysis of the GeV gamma-ray emission toward the supernova remnant (SNR) G8.7–0.1 with the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope. An investigation of the relationship between G8.7–0.1 and the TeV unidentified source HESS J1804–216 provides us with an important clue on diffusion process of cosmic rays if particle acceleration operates in the SNR. The GeV gamma-ray emission is extended with most of the emission in positional coincidence with the SNR G8.7–0.1 and a lesser part located outside the western boundary of G8.7–0.1. The region of the gamma-ray emission overlaps spatially connected molecular clouds, implying a physical connection for the gamma-ray structure. The total gamma-ray spectrum measured with LAT from 200 MeV-100 GeV can be described by a broken power-law function with a break of 2.4 ± 0.6 (stat) ± 1.2 (sys) GeV, and photon indices of 2.10 ± 0.06 (stat) ± 0.10 (sys) below the break and 2.70 ± 0.12 (stat) ± 0.14 (sys) above the break. Given the spatial association among the gamma rays, the radio emission of G8.7–0.1, and the molecular clouds, the decay of π 0 s produced by particles accelerated in the SNR and hitting the molecular clouds naturally explains the GeV gamma-ray spectrum. We also find that the GeV morphology is not well represented by the TeV emission from HESS J1804–216 and that the spectrum in the GeV band is not consistent with the extrapolation of the TeV gamma-ray spectrum. The spectral index of the TeV emission is consistent with the particle spectral index predicted by a theory that assumes energy-dependent diffusion of particles accelerated in an SNR. We discuss the possibility that the TeV spectrum originates from the interaction of particles accelerated in G8.7–0.1 with molecular clouds, and we constrain the diffusion coefficient of the particles.

Quantitative Evaluation of Tumor Early Response to a Vascular-Disrupting Agent with Dynamic PET.

Science.gov (United States)

Guo, Ning; Zhang, Fan; Zhang, Xiaomeng; Guo, Jinxia; Lang, Lixin; Kiesewetter, Dale O; Niu, Gang; Li, Quanzheng; Chen, Xiaoyuan

2015-12-01

The purpose of this study is to evaluate the early response of tumors to a vascular-disrupting agent (VDA) VEGF121/recombinant toxin gelonin (rGel) using dynamic [(18)F]FPPRGD2 positron emission tomography (PET) and kinetic parameter estimation. Two tumor xenograft models: U87MG (highly vascularized) and A549 (moderately vascularized), were selected, and both were randomized into treatment and control groups. Sixty-minute dynamic PET scans with [(18)F]FPPRGD2 that targets to integrin αvβ3 were performed at days 0 (baseline), 1, and 3 since VEGF121/rGel treatment started. Dynamic PET-derived binding potential (BPND) and parametric maps were compared with tumor uptake (%ID/g) and the static PET image at 1 h after the tracer administration. The growth of U87MG tumor was obviously delayed upon VEGF121/rGel treatment. A549 tumor was not responsive to the same treatment. BPND of treated U87MG tumors decreased significantly at day 1 (p dynamic PET with [(18)F]FPPRGD2 shows advantages in distinguishing effective from ineffective treatment during the course of VEGF121/rGel therapy at early stage and is therefore more sensitive in assessing therapy response than static PET.

Characterization and Prognosis Significance of JAK2 (V617F), MPL, and CALR Mutations in Philadelphia-Negative Myeloproliferative Neoplasms

Science.gov (United States)

Singdong, Roongrudee; Siriboonpiputtana, Teerapong; Chareonsirisuthigul, Takol; Kongruang, Adcharee; Limsuwanachot, Nittaya; Sirirat, Tanasan; Chuncharunee, Suporn; Rerkamnuaychoke, Budsaba

2016-10-01

Background: The discovery of somatic acquired mutations of JAK2 (V617F) in Philadelphia-negative myeloproliferative neoplasms (Ph-negative MPNs) including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF) has not only improved rational disease classification and prognostication but also brings new understanding insight into the pathogenesis of diseases. Dosage effects of the JAK2 (V617F) allelic burden in Ph-negative MPNs may partially influence clinical presentation, disease progression, and treatment outcome. Material and Methods: Pyrosequencing was performed to detect JAK2 (V617F) and MPL (W515K/L) and capillary electrophoresis to identify CALR exon 9.0 mutations in 100.0 samples of Ph-negative MPNs (38.0 PV, 55 ET, 4 PMF, and 3 MPN-U). Results: The results showed somatic mutations of JAK2 (V617F) in 94.7% of PV, 74.5% of ET, 25.0% of PMF, and all MPN-U. A high proportion of JAK2 (V617F) mutant allele burden (mutational load > 50.0%) was predominantly observed in PV when compared with ET. Although a high level of JAK2 (V617F) allele burden was strongly associated with high WBC counts in both PV and ET, several hematological parameters (hemoglobin, hematocrit, and platelet count) were independent of JAK2 (V617F) mutational load. MPL (W515K/L) mutations could not be detected whereas CALR exon 9.0 mutations were identified in 35.7% of patients with JAK2 negative ET and 33.3% with JAK2 negative PMF. Conclusions: The JAK2 (V617F) allele burden may be involved in progression of MPNs. Furthermore, a high level of JAK2 (V617F) mutant allele appears strongly associated with leukocytosis in both PV and ET. Creative Commons Attribution License

Comparative response of platelet fV and plasma fV to activated protein C and relevance to a model of acute traumatic coagulopathy.

Directory of Open Access Journals (Sweden)

James E Campbell

Full Text Available BACKGROUND: Acute traumatic coagulopathy (ATC has been linked to an increase in activated protein C (aPC from 40 pM in healthy individuals to 175 pM. aPC exerts its activity primarily through cleavage of active coagulation factor Va (fVa. Platelets reportedly possess fVa which is more resistant to aPC cleavage than plasma fVa; this work examines the hypothesis that normal platelets are sufficient to maintain coagulation in the presence of elevated aPC. METHODS: Coagulation responses of normal plasma, fV deficient plasma (fVdp, and isolated normal platelets in fVdp were conducted: prothrombin (PT tests, turbidimetry, and thromboelastography (TEG, including the dose response of aPC on the samples. RESULTS: PT and turbidimetric assays demonstrate that normal plasma is resistant to aPC at doses much higher than those found in ATC. Additionally, an average physiological number of washed normal platelets (200,000 platelets/mm3 was sufficient to eliminate the anti-coagulant effects of aPC up to 10 nM, nearly two orders of magnitude above the ATC concentration and even the steady-state pharmacological concentration of human recombinant aPC, as measured by TEG. aPC also demonstrated no significant effect on clot lysis in normal plasma samples with or without platelets. CONCLUSIONS: Although platelet fVa shows slightly superior resistance to aPC's effects compared to plasma fVa in static models, neither fVa is sufficiently cleaved in simulations of ATC or pharmacologically-delivered aPC to diminish coagulation parameters. aPC is likely a correlative indicator of ATC or may play a cooperative role with other activity altering products generated in ATC.

Analysis, design and implementation of sensorless V/f control in a ...

Indian Academy of Sciences (India)

SOURABH PAITANDI

Keywords. Surface-mounted PMSM; V/f control; sensorless control; stabilisation of PMSM; efficiency optimisation. ..... The power balance equation can be written as, pe ¼ Pe ю .... loading the actual speed falls for a very short transient period.

Hanford Waste Vitrification Project Building limited scope risk assessment

International Nuclear Information System (INIS)

Braun, D.J.; Lindberg, S.E.; Reardon, M.F.; Wilson, G.P.

1992-10-01

A limited scope risk assessment was performed on the preliminary design of a high-level waste interim storage facility. The Canister Storage Building (CSB) facility will be built to support remediation at the US Department of Energy Hanford Site in Washington State. The CSB will be part of the support facilities for a high level Hanford Waste Vitrification Plant (HWVP). The limited scope risk assessment is based on a preliminary design which uses forced air circulation systems to move air through the building vault. The current building design calls for natural circulation to move air through the building vault

Preparation of 3-7 MeV neutron source and preliminary results of activation cross section measurement

Energy Technology Data Exchange (ETDEWEB)

Furuta, T.; Masuda, T.; Tsurita, Y.; Hashimoto, A.; Miyajima, N. [Department of Nuclear Engineering, Nagoya University, Nagoya, Aichi (Japan); Sakane, H.; Shibata, M.; Yamamoto, H.; Kawade, K.

1999-03-01

A d-D gas target producing monoenergetic neutrons has been constructed for measurement of activation cross sections in the energy region of 3 to 7 MeV at Van de Graaff accelerator of Nagoya University. Neutron spectra and neutron fluxes were measured as a function of the incident deuteron energy. Preliminary results of activation cross sections were obtained for reactions {sup 27}Al(n, p){sup 27}Mg, {sup 27}Al(n, {alpha}){sup 24}Na, {sup 47}Ti(n, p){sup 47}Sc, {sup 56}Fe(n, p){sup 56}Mn, {sup 58}Ni(n, p){sup 58}Co and {sup 64}Zn(n, p){sup 64}Cu. The results are compared with the evaluated values of JENDL-3.2. A well-type HPGe detector was used for highly efficient detection. (author)

Functional correlates of TSH, fT3 and fT4 in Alzheimer disease: a F-18 FDG PET/CT study.

Science.gov (United States)

Chiaravalloti, Agostino; Ursini, Francesco; Fiorentini, Alessandro; Barbagallo, Gaetano; Martorana, Alessandro; Koch, Giacomo; Tavolozza, Mario; Schillaci, Orazio

2017-07-24

The present study was aimed to investigate the relationships between thyroid stimulating hormone (TSH), freeT3 (fT3) and freeT4 (fT4) and brain glucose consumption as detectable by means of 2-deoxy-2-(F-18) fluoro-D-glucose (F-18 FDG) Positron Emission Tomography/Computed Tomography (PET/CT) in a selected population with Alzheimer disease (AD). We evaluated 87 subjects (37 males and 50 females, mean age 70 (±6) years old) with AD. All of them were subjected to TSH, fT3 and fT4 assay and to cerebrospinal fluid amyloid (Aβ1-42) and tau [phosphorylated-tau (p-tau) and total-tau (t-tau)] assay prior PET/CT examination. Values for TSH, fT3 and fT4 were in the normal range. The relationships were evaluated by means of statistical parametric mapping (SPM8) using age, sex, MMSE, scholarship and CSF values of amyloid and tau as covariates. We found a significant positive correlation between TSH values and cortical glucose consumption in a wide portion of the anterior cingulate cortex bilaterally (BA32) and left frontal lobe (BA25) (p FWE-corr <0.001; p FDRcorr <0.000; cluster extent 66950). No significant relationships were found between cortical F-18 FDG uptake and T3 and T4 serum levels. The results of our study suggest that a cortical dysfunction in anterior cingulate and frontal lobes may affect serum values of TSH in AD patients.

Relationship between ionospheric F2-layer critical frequency, F10.7, and F10.7P around African EIA trough

Science.gov (United States)

Ikubanni, S. O.; Adeniyi, J. O.

2017-02-01

Improved ionospheric modeling requires a better understanding of the relationship between ionospheric parameters and their influencing solar and geomagnetic sources. Published reports of the validation of the International Reference Ionosphere (IRI) for quiet-time revealed either underestimation or overestimation at a greater magnitude during high solar fluxes, especially at low latitude. With daily foF2 data from Ouagadougou (geor. 12.4°N, 1.5°W) covering a solar cycle, we have presented preliminary results from the analysis of solar dependence of six different classifications of the data: (i) daily values, (ii) monthly mean, (iii) daily quiet values (with Ap ⩽ 20), (iv) monthly-quiet-mean values, (v) monthly median, and (vi) monthly-quiet-median values. All six classifications show good nonlinear relationship with both F10.7 and F10.7P, however, the differences between the dependence of classes (i) and (iii) of foF2 on the two solar indices is more substantial than those of classes (ii), (iv), (v), and (vi). Of all the six classes, the monthly averages are best related to both solar activity indices. Further analysis shows that magnetic disturbances are non-influential in the variations of the monthly mean of both solar activity indices; this makes both good indices for quiet-time modeling. Likewise, F10.7 and F10.7P are indistinguishable for long-term modeling around the African EIA trough region. While monthly median values may be best for mid-latitude region, either the mean/median values could be used for low-latitude region. However, it could be worthwhile to examine the distribution of the data from the station under consideration.

Frequency and clinical features of the JAK2 V617F mutation in pediatric patients with sporadic essential thrombocythemia.

Science.gov (United States)

Nakatani, Takuya; Imamura, Toshihiko; Ishida, Hiroyuki; Wakaizumi, Katsuji; Yamamoto, Tohru; Otabe, Osamu; Ishigami, Tsuyoshi; Adachi, Souichi; Morimoto, Akira

2008-12-01

Pediatric essential thrombocythemia (ET) is a rare and heterogenous disease entity. While several recent studies have focused on the role of the JAK2 V617F mutation in pediatric ET, the frequency of pediatric ET cases with this mutation and the associated clinical features remain unclear. We examined six childhood cases who had been diagnosed with ET according to WHO criteria (onset age: 0.2-14 years) for the presence of the JAK2 V617F mutation, MPLW515L mutation and JAK2 exon 12 mutations. Two sensitive PCR-based methods were used for the JAK2 V617F genotyping. We also examined the expression of polycythemia rubra vera-1 (PRV-1), which is a diagnostic marker for clonal ET. We found that three of the six cases had the JAK2 V617F mutation and that all six cases expressed PRV-1 in their peripheral granulocytes. Neither MPL W515L mutation nor JAK2 exon 12 mutations was detected in the patients without JAK2 V617F mutation. The two patients who developed thrombocythemia during infancy were JAK2 V617F-negative. These findings suggest that the JAK2 V617F mutation is not rare in childhood sporadic ET cases, and that these cases might be older and myeloproliferative features.

Noble metals-compatible melter features development Phase 1: Establishing functional and design criteria and design concepts

International Nuclear Information System (INIS)

Elmore, M.R.; Siemens, D.H.; Chapman, C.C.

1996-03-01

Premature failures have occurred in melters at Japan's Tokai Mockup Facility and at the Federal Republic of Germany (FRG) PAMELA plant during processing of feeds with high levels of noble metals. Melter failure was due to the accumulation of an electrically conductive, noble metals-containing precipitates in the glass, that then resulted in short circuiting of the electrodes. A comparison was made of the anticipated Hanford Waste Vitrification Plant (HWVP) feed with the feeds processed in the FRG and Japanese melters. The evaluation showed that comparable levels of noble metals and other potential precipitate-forming components (e.g. Cr/Fe/Ni-spinels) exist in the HWVP feed. As a result, the HWVP project made a decision to modify the present reference melter design to include features to prevent the precipitation and accumulation or otherwise accommodate precipitated phases on a routine basis without loss of production capacity

Effects of Load and Speed Variations in a Modified Closed Loop V/F ...

African Journals Online (AJOL)

This paper investigates the eects of load and reference speed variations in a modied closed loopv=f induction motor drive. A modied approach, involving the addition of a low frequency boostvoltage, is developed and adopted as an enhancement to the conventional closed loop v=f speedcontrol of a three phase squirrel ...

Effect of Wood Aging on Wine Mineral Composition and 87Sr/86Sr Isotopic Ratio.

Science.gov (United States)

Kaya, Ayse D; Bruno de Sousa, Raúl; Curvelo-Garcia, António S; Ricardo-da-Silva, Jorge M; Catarino, Sofia

2017-06-14

The evolution of mineral composition and wine strontium isotopic ratio 87 Sr/ 86 Sr (Sr IR) during wood aging were investigated. A red wine was aged in stainless steel tanks with French oak staves (Quercus sessiliflora Salisb.), with three industrial scale replicates. Sampling was carried out after 30, 60, and 90 days of aging, and the wines were evaluated in terms of general analysis, phenolic composition, total polysaccharides, multielement composition, and Sr IR. Li, Be, Mg, Al, Sc, Ti, V, Mn, Co, Ni, Cu, Zn, Ga, Ge, As, Rb, Sr, Y, Zr, Mo, Sb, Cs, Ba, Pr, Nd, Sm, Eu, Dy, Ho, Er, Yb, Lu, Tl, and Pb elements and 87 Sr/ 86 Sr were determined by quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS) and Na, K, Ca, and Fe by flame atomic absorption spectrometry (FAAS). Two-way ANOVA was applied to assess wood aging and time effect on Sr IR and mineral composition. Wood aging resulted in significantly higher concentrations of Mg, V, Co, Ni, and Sr. At the end of the aging period, wine exhibited statistically identical Sr IR compared to control. Study suggests that wood aging does not affect 87 Sr/ 86 Sr, not precluding the use of this parameter for wine traceability purposes.

High resolution 2-D imaging polarimetry of the jet of M87, in the light of U, B and V

Energy Technology Data Exchange (ETDEWEB)

Visvanathan, N; Pickles, A J [Australian National Univ., Canberra. Mount Stromlo and Siding Spring Observatories

1981-01-01

Polarisation maps to a resolution of 1 arcsec in the jet of M87 are presented. The results show that small-scale structure exists in the magnetic field down to at least this resolution, as evidenced by the fact that the 1 arcsec resolution percentage polarisations are approximately 80% higher than those obtained at a lower resolution, 2 arcsec. The sudden change of position angles of the electric vector near knot B supports shock models of the jet. The jet is significantly broader in the light of U than in B or V. The continuous energy distribution in UBV can be described as a power law spectrum, with a slope of -1.7 +- 0.3 which indicates a turnover in the synchrotron spectrum in the vicinity of V.

Synthesis and evaluation of [[sup 18]F]fluoroprogestins and [[sup 18]F]fluorometoprolol

Energy Technology Data Exchange (ETDEWEB)

De Groot, T J

1993-05-01

The author investigated if specific radioactively labelled compounds could be applied to gain insight into particular psychic diseases, f.e. Parkinson's disease and schizophrenia, by means of Positron Emission Tomography (PET). No appropriate compounds were found. In this thesis the syntheses of fluorine-18 labelled progestins and [beta][sub 1]-adrenergic ligands are described. Three approaches towards [[sup 18]F]fluorination are investigated. The first method concerns direct S[sub N]2-substitution, the second approach is the opening of an epoxide, and the third approach is [[sup 18]F]fluoroalkylation. The positron emitting radionuclide fluorine-18 was used because of its relatively long decay time and the possibility to produce it in high yields and with high specific activity. The target systems which were applied for the production of fluorine-18 are described in chapter two. Important chemical and physical aspects of [[sup 18]F]fluoride are reviewed in the same chapter. In chapter three the synthesis of 21-[[sup 18]F]fluorinated progestins is discussed. The synthesis of four 21-[[sup 18]F]fluoroprogesterone derivatives is described and the results of an in vivo evaluation of two of these ligands are discussed. Possible routes leading to 6[alpha]-[[sup 18]F]fluoroprogestins are presented in chapter four. The radiochemical approaches towards the synthesis of these ligands are discussed. In chapter five the proposed routes to the fluorine-18 labelled [beta][sub 1]-adrenergic ligands are described and evaluated in the synthesis of two model compounds. 1-[[sup 18]F]fluorometoprolol, the [[sup 18]F]fluorinated analogue of a potent beta-blocker, is prepared using one of the investigated methods. The biological effect of fluorine substitution of a [beta][sub 1]-adrenergic ligand is discussed on the basis of an in vitro and in vivo evaluation. 21 figs., 28 schemes, 19 tabs., 182 refs.

Low temperature synthesis and characterization of Na–M–(O)–F phases with M=Ti, V

Energy Technology Data Exchange (ETDEWEB)

Nava-Avendaño, Jessica [Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, E-08193 Bellaterra, Catalonia (Spain); Ayllón, José A. [Departament de Química, Universitat Autònoma de Barcelona, Campus UAB, E-08193 Bellaterra, Catalonia (Spain); Frontera, Carlos; Oró-Solé, Judith [Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, E-08193 Bellaterra, Catalonia (Spain); Estruga, Marc [Departament de Química, Universitat Autònoma de Barcelona, Campus UAB, E-08193 Bellaterra, Catalonia (Spain); Molins, Elies [Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, E-08193 Bellaterra, Catalonia (Spain); Palacín, M. Rosa, E-mail: rosa.palacin@icmab.es [Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, E-08193 Bellaterra, Catalonia (Spain)

2015-03-15

Na{sub 5}Ti{sub 3}O{sub 3}F{sub 11} was prepared by the microwave assisted method, and presents a chiolite related structure with cell parameters a=10.5016(5), b=10.4025(5), and c=10.2911(5) Å and Cmca (no. 64) space group. From solvothermal synthesis at 100 °C the cryolite Na{sub 3−δ}VO{sub 1−δ}F{sub 5+δ} was prepared, which crystallizes in the monoclinic system with a=5.5403(2), b=5.6804(2), c=7.9523(2) Å, β=90.032(7)° cell parameters and P2{sub 1}/n (no. 14) space group. Under similar synthesis conditions but with higher HF concentration the chiolite-type phase Na{sub 5−δ}V{sub 3}F{sub 14} was achieved, which exhibits a=10.5482(2), b=10.4887(1) and c=10.3243(1) Å cell parameters and Cmc2{sub 1} (no. 36) space group. A single crystal also having the chiolite structure was synthesized at 200 °C which exhibits tetragonal symmetry (a=7.380(3) and c=10.381(11) Å and space group P4{sub 2}2{sub 1}2 (no. 94)). Bond valence sum indicates that it contains V{sup 4+} and therefore can be formulated as Na{sub 5}V{sub 3}O{sub 3}F{sub 11}. - Graphical abstract: Na{sub 5}M{sub 3}(O,F){sub 14} with M=Ti and V having chiolite structure and Na{sub 3−δ}VO{sub 1−δ}F{sub 5+δ} cryolite were prepared by means of microwave-assisted and solvothermal synthesis. - Highlights: • Na{sub 5}Ti{sub 3}O{sub 3}F{sub 11} chiolite was prepared by a microwave assisted method and characterized. • Na{sub 3−δ}VO{sub 1−δ}F{sub 5+δ} and Na{sub 5−δ}V{sub 3}F{sub 14} were prepared by solvothermal synthesis. • The compounds were structurally characterized by diffraction techniques. • O/F distribution was estimated by applying Pauling’s second rule.

The V279F polymorphism might change protein character and ...

African Journals Online (AJOL)

Polymorphisms at the protein level are also estimated to correlate with increased risk factors for heart attacks. One such polymorphism is the V279F polymorphism in Lp-PLA2 which results in a change in enzyme performance capability. This in turn implies a reduced risk of acute myocardial infarct (AMI) in Korean and ...

Noninvasive imaging of tumor integrin expression using 18F-labeled RGD dimer peptide with PEG4 linkers

International Nuclear Information System (INIS)

Liu, Zhaofei; Liu, Shuanglong; Wang, Fan; Liu, Shuang; Chen, Xiaoyuan

2009-01-01

Various radiolabeled Arg-Gly-Asp (RGD) peptides have been previously investigated for tumor integrin α v β 3 imaging. To further develop RGD radiotracers with enhanced tumor-targeting efficacy and improved in vivo pharmacokinetics, we designed a new RGD homodimeric peptide with two PEG 4 spacers (PEG 4 = 15-amino-4,7,10,13-tetraoxapentadecanoic acid) between the two monomeric RGD motifs and one PEG 4 linker on the glutamate α-amino group ( 18 F-labeled PEG 4 -E[PEG 4 -c(RGDfK)] 2 , P-PRGD2), as a promising agent for noninvasive imaging of integrin expression in mouse models. P-PRGD2 was labeled with 18 F via 4-nitrophenyl 2- 18 F-fluoropropionate ( 18 F-FP) prosthetic group. In vitro and in vivo characteristics of the new dimeric RGD peptide tracer 18 F-FP-P-PRGD2 were investigated and compared with those of 18 F-FP-P-RGD2 ( 18 F-labeled RGD dimer without two PEG 4 spacers between the two RGD motifs). The ability of 18 F-FP-P-PRGD2 to image tumor vascular integrin expression was evaluated in a 4T1 murine breast tumor model. With the insertion of two PEG 4 spacers between the two RGD motifs, 18 F-FP-P-PRGD2 showed enhanced integrin α v β 3 -binding affinity, increased tumor uptake and tumor-to-nontumor background ratios compared with 18 F-FP-P-RGD2 in U87MG tumors. MicroPET imaging with 18 F-FP-P-PRGD2 revealed high tumor contrast and low background in tumor-bearing nude mice. Biodistribution studies confirmed the in vivo integrin α v β 3 -binding specificity of 18 F-FP-P-RGD2. 18 F-FP-P-PRGD2 can specifically image integrin α v β 3 on the activated endothelial cells of tumor neovasculature. 18 F-FP-P-PRGD2 can provide important information on integrin expression on the tumor vasculature. The high integrin binding affinity and specificity, excellent pharmacokinetic properties and metabolic stability make the new RGD dimeric tracer 18 F-FP-P-PRGD2 a promising agent for PET imaging of tumor angiogenesis and for monitoring the efficacy of antiangiogenic

[JAK2 V617F and exon 12 genetic variations in Korean patients with BCR/ABL1-negative myeloproliferative neoplasms].

Science.gov (United States)

Kim, Jeong Tae; Cho, Yong Gon; Choi, Sam Im; Lee, Young Jin; Kim, Hye Ran; Jang, Sook Jin; Moon, Dae Soo; Park, Young Jin; Park, Geon

2010-12-01

JAK2 genetic variations have been described in a high proportion of patients with BCR/ABL1-negative myeloproliferative neoplasms (MPN). This study was designed to analyze the frequencies of JAK2 V617F and exon 12 variations, and their correlations with clinical characteristics of Korean patients with BCR/ABL1-negative MPN. We examined a total of 154 patients with BCR/ABL1-negative MPN that included 24, 26, 89, and 15 patients with polycythemia vera (PV), primary myelofibrosis (PMF), essential thrombocythemia (ET), and unclassified myeloproliferative neoplasms (MPNU), respectively. We performed allele-specific PCR to detect V617F in all BCR/ABL1-negative patients, and performed direct sequencing to detect exon 12 variations in 47 V617F-negative MPN patients. JAK2 c.1641+179_183del5 variation was detected by restriction fragment length polymorphism assay in 176 healthy subjects. JAK2 V617F was detected in 91 patients (59.1%): PV (91.6%), PMF (46.2%), ET (52.8%), and MPNU (66.7%). In V617F-negative MPN patients, no mutations were found in exon 12. The c.1641+179_183del5 was detected in 68.1% of V617F-negative MPN patients and 45.4% of healthy subjects (P=0.008). JAK2 V617F was closely correlated with age and leukocytosis in BCR/ABL1-negative MPN patients (P<0.05). However, c.1641+179_183del5 was not related to age, sex, or complete blood cell count parameters in V617F-negative MPN patients and healthy subjects. The c.1641+179_183del5 was associated with an increased odds ratio for MPN (odds ratio, 2.6; 95% confidences interval, 1.3-5.1; P=0.007). Frequencies of V617F are similar to reported results. JAK2 exon 12 mutations may be rare and c.1641+179_183del5 may influence the occurrence of MPN in Korean patients with V6 17F-negative MPN.

Effect of time in the evaluation of F+20 and F0 diuretic renography

International Nuclear Information System (INIS)

Donoso, G.; Piepsz, A.; Ham, H.

2002-01-01

F0 diuretic renography starts gaining popularity especially because of the practical convenient of avoiding one intravenous injection. Using F0 however, the most adequate parameters for evaluating renal output are still to be determined. Aim: to evaluate which parameter of F0 and F+20 procedures gives rise to the best possible renal drainage. Methods: In this retrospective study we compared F0 and F20 quantitative parameters obtained in 36 clinically stable children, aged 10 days to 17 years, with unilateral (25) or bilateral (11)hydronephrosis, in whom both tests were performed. The mean time interval between the two tests was 17 months. F0 and F+20 Tc-99m MAG3 diuretic renography were carried out using the usual procedure. In all cases, a late image (PM) was acquired after micturition and after changing the position of the patient. The mean time at which this image was acquired was 80 min(SD : 33) for the F+20 test and 48 min(SD : 30) for the F0 test. Following parameters were calculated : Time to the maximum of the basic renogram (Tmax); normalized residual activity (NORA) and output efficiency (OE) at the end of the 20- min renogram, at the end of furosemide test (35 min) and on the post micturition image (NORA 20, NORA 35, NORA PM, OE 20, OE35 and OE PM). On the basis of all available parameters, each of the 47 hydronephrotic kidneys were classified either as 'good drainage' or as 'partial or bad drainage'. All parameters were then evaluated according to this reference. Conclusions: For both the F+20 and the F0 tests, the PM image had obviously a higher performance rate than all the other parameters, underlying the mandatory character of this acquisition. The higher performance of the PM image on F+20 is clearly related to the significantly different time at which the PM image was acquired, underlying the need of standardization of the acquisition time

Preliminary Experiments for the Assessment of V/W-band Links for Space-Earth Communications

Science.gov (United States)

Nessel, James A.; Acosta, Roberto J.; Miranda, Felix A.

2013-01-01

Since September 2012, NASA Glenn Research Center has deployed a microwave profiling radiometer at White Sands, NM, to estimate atmospheric propagation effects on communications links in the V and W bands (71-86GHz). Estimates of attenuation statistics in the millimeter wave due to gaseous and cloud components of the atmosphere show good agreement with current ITU-R models, but fail to predict link performance in the presence of moderate to heavy rain rates, due to the inherent limitations of passive radiometry. Herein, we discuss the preliminary results of these measurements and describe a design for a terrestrial link experiment to validate/refine existing rain attenuation models in the V/Wbands.

JAK2 46/1 haplotype is associated with JAK2 V617F--positive myeloproliferative neoplasms in Brazilian patients.

Science.gov (United States)

Macedo, L C; Santos, B C; Pagliarini-e-Silva, S; Pagnano, K B B; Rodrigues, C; Quintero, F C; Ferreira, M E; Baraldi, E C; Ambrosio-Albuquerque, E P; Sell, A M; Visentainer, J E L

2015-10-01

This study aimed to verify the association between the JAK2 46/1 haplotype (V617F positive) and some hematological parameters in BCR-ABL-negative chronic myeloproliferative neoplasms (cMPNs) in our population. The blood samples obtained from the patients with cMPN were genotyped for the JAK2 V617F mutation and JAK2 rs10974944 SNP screening using a PCR-RFLP assay. The JAK2 V617F mutation was detected in 80.15% of patients. The G variant of rs10974944 was more frequent in all MPNs, especially those that were JAK2 V617F positive, than in the control population. We also compared the 46/1 haplotype status in each MPN disease entity, polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis (PMF), and MPNu with controls. The G allele frequency relative to controls was significantly enriched in patients with PV and ET, but not in those with PMF and MPNu. PV and ET patients especially, all of whom had the JAK2 V617F mutation, showed significant excess of the G allele. The frequency of JAK2 V617F mutation was associated with elevated hematological parameters, but when we analyze the occurrence of the mutation and the presence of the G allele, just the high hemoglobin was significantly. In agreement with previous reports, JAK2 46/1 haplotype for JAK2 V617F was associated with cMPN positive in Brazilian patients. © 2015 John Wiley & Sons Ltd.

Quantitative analysis and comparison study of [18F]AlF-NOTA-PRGD2, [18F]FPPRGD2 and [68Ga]Ga-NOTA-PRGD2 using a reference tissue model.

Directory of Open Access Journals (Sweden)

Ning Guo

Full Text Available With favorable pharmacokinetics and binding affinity for α(vβ(3 integrin, (18F-labeled dimeric cyclic RGD peptide ([(18F]FPPRGD2 has been intensively used as a PET imaging probe for lesion detection and therapy response monitoring. A recently introduced kit formulation method, which uses an (18F-fluoride-aluminum complex labeled RGD tracer ([(18F]AlF-NOTA-PRGD2, provides a strategy for simplifying the labeling procedure to facilitate clinical translation. Meanwhile, an easy-to-prepare (68Ga-labeled NOTA-PRGD2 has also been reported to have promising properties for imaging integrin α(vβ(3. The purpose of this study is to quantitatively compare the pharmacokinetic parameters of [(18F]FPPRGD2, [(18F]AlF-NOTA-PRGD2, and [(68Ga]Ga-NOTA-PRGD2. U87MG tumor-bearing mice underwent 60-min dynamic PET scans following the injection of three tracers. Kinetic parameters were calculated using Logan graphical analysis with reference tissue. Parametric maps were generated using voxel-level modeling. All three compounds showed high binding potential (Bp(ND = k(3/k(4 in tumor voxels. [(18F]AlF-NOTA-PRGD2 showed comparable Bp(ND value (3.75±0.65 with those of [(18F]FPPRGD2 (3.39±0.84 and [(68Ga]Ga-NOTA-PRGD2 (3.09±0.21 (p>0.05. Little difference was found in volume of distribution (V(T among these three RGD tracers in tumor, liver and muscle. Parametric maps showed similar kinetic parameters for all three tracers. We also demonstrated that the impact of non-specific binding could be eliminated in the kinetic analysis. Consequently, kinetic parameter estimation showed more comparable results among groups than static image analysis. In conclusion, [(18F]AlF-NOTA-PRGD2 and [(68Ga]Ga-NOTA-PRGD2 have comparable pharmacokinetics and quantitative parameters compared to those of [(18F]FPPRGD2. Despite the apparent difference in tumor uptake (%ID/g determined from static images and clearance pattern, the actual specific binding component extrapolated from kinetic

Properties of the 4.45 eV optical absorption band in LiF:Mg, Ti

International Nuclear Information System (INIS)

Nail, I.; Oster, L.; Horowitz, Y. S.; Biderman, S.; Belaish, Y.

2006-01-01

The optical absorption (OA) and thermoluminescence (TL) of dosimetric LiF:Mg,Ti (TLD-100) as well as nominally pure LiF single crystal have been studied as a function of irradiation dose, thermal and optical bleaching in order to investigate the role of the 4.45 eV OA band in low temperature TL. Computerised deconvolution was used to resolve the absorption spectrum into individual gaussian bands and the TL glow curve into glow peaks. Although the 4.45 eV OA band shows thermal decay characteristics similar to the 4.0 eV band its dose filling constant and optical bleaching properties suggest that it cannot be associated with the TL of composite peaks 4 or 5. Its presence in optical grade single crystal LiF further suggests that it is an intrinsic defect or possibly associated with chance impurities other than Mg, Ti. (authors)

1.5-V-threshold-voltage Schottky barrier normally-off AlGaN/GaN high-electron-mobility transistors with f T/f max of 41/125 GHz

Science.gov (United States)

Hou, Bin; Ma, Xiaohua; Yang, Ling; Zhu, Jiejie; Zhu, Qing; Chen, Lixiang; Mi, Minhan; Zhang, Hengshuang; Zhang, Meng; Zhang, Peng; Zhou, Xiaowei; Hao, Yue

2017-07-01

In this paper, a normally-off AlGaN/GaN high-electron-mobility transistors (HEMT) fabricated using inductively coupled plasma (ICP) CF4 plasma recessing and an implantation technique is reported. A gate-to-channel distance of ˜10 nm and an equivalent negative fluorine sheet charge density of -1.21 × 1013 cm-2 extracted using a simple threshold voltage (V th) analytical model result in a high V th of 1.5 V, a peak transconductance of 356 mS/mm, and a subthreshold slope of 133 mV/decade. A small degradation of channel mobility leads to a high RF performance with f T/f max of 41/125 GHz, resulting in a record high f T × L g product of 10.66 GHz·µm among Schottky barrier AlGaN/GaN normally-off HEMTs with V th exceeding 1 V, to the best of our knowledge.

Semi-automatic synthesis and biological evaluation of 18F-FCH as an oncologic PET tracer

International Nuclear Information System (INIS)

Wu Zhanhong; Wang Shizhen; Zhou Qian; Fu Zhe; Qiu Feichan; Huo Li

2005-01-01

18 F-fluromethylcholine ( 18 F-FCH) as a PET tracer is synthesized. The semi-automatic synthesis assembly of 18 F-FCH is modified from CPCU(CTI). The radiochemical purity is measured by analytical HPLC. The radiochemical yield and the radiochemical purity of 18 F-FCH are 15% and >99%, respectively. The total radiosynthesis time is 55 min after EOB. The labeled product exhibited low toxicity. The biodistribution in normal mice and the toxicity are studied. PET imaging with 18 F-FCH is performed on tumor xenograft murine model. The semi-automatic synthesis assembly is promising to be used for routine clinic radiopharmaceutical preparation and preliminary study has shown the usefulness of 18 F-FCH as an oncologic PET tracer. (authors)

Large-x sub F spin asymmetry in. pi. sup 0 production by 200-GeV polarized protons

Energy Technology Data Exchange (ETDEWEB)

Adams, D L; Corcoran, M D; Cranshaw, J; Nessi-Tedaldi, F; Nessi, M; Nguyen, C; Roberts, J B; Skeens, J; White, J L [Rice Univ., Houston, TX (United States). T.W. Bonner Nuclear Lab.; Akchurin, N; Onel, Y [Iowa Univ., Iowa City, IA (United States). Dept. of Physics; Belikov, N I; Derevschikov, A A; Grachov, O A; Matulenko, Yu A; Meschanin, A P; Nurushev, S B; Patalakha, D I; Rykov, V L; Solovyanov, V L; Vasiliev, A N [Inst. of High Energy Physics, Serpukhov (Russia); Bystricky, J; Lehar, F; Lesquen, A de [CEN-Saclay, 91 - Gif-sur-Yvette (France); Cossairt, J D; Read, A L [Fermi National Accelerator Lab., Batavia, IL (United States); En' yo, H; Funahashi, H; Goto, Y; Imai, K; Itow, Y; Makino, S; Masaike, A; Miyake, K; Nagamine, T; Saito, N; Yamashita, S [Kyoto Univ. (Japan). Dept. of Physics; Grosnick, D P; Hill, D A; Laghai, M; Lopiano, D; Ohashi, Y; Spinka, H; Underwood, D G; Yokosawa, A [Argonne National Lab., IL (United States); FNAL E704 Collaboration

1992-10-01

The spin asymmetry A{sub N} for inclusive {pi}{sup 0} production by 200-GeV transversely-polarized protons on a liquid hydrogen target has been measured at Fermilab over a wide range of x{sub F}, with 0.5V/c. At x{sub F}>0.3, the asymmetry rises with increasing x{sub F} and reaches a value of A{sub N}=0.15{+-}0.03 in the region 0.6F}<0.8. This result provides new input regarding the question of the internal spin structure of transversely-polarized protons. (orig.).

Evaluation of Ti-6Al-4V surface treatments for use with a polyphenylquinoxaline adhesive

Science.gov (United States)

Progar, Donald J.

1987-01-01

Three surface treatments for Ti-6Al-4V adherends were evaluated using a thermoplastic polymer monoether polyphenylquinoxaline, MEPPQ, which had been shown in previous studies to have good potential as a high temperature adhesive for aerospace applications. Initial results based on long term thermal exposure at 232 C (450 F) using the phosphate-fluoride (PF) and chromic acid anodized (CAA) treatments with MEPPQ adhesive were not encouraging. A significant improvement in strength retention and a change in failure mode (cohesive) at 232 C (450 F) was found for the SHA treated specimens compared to the PF and CAA treatments. Although an improvement in long term thermal durability was obtained with the SHA treatment of Ti-6Al-4V, an improved surface treatment with better long term durability is still required for aerospace applications.

AFSC/ABL: Origins of salmon seized from the F/V Arctic Wind

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Samples of chum (Oncorhynchus keta), sockeye (O. nerka), and chinook salmon (O. tshawytscha) seized from the F/V Arctic Wind were analyzed to determine their region...

238U (n,f) measurements below 30 keV

International Nuclear Information System (INIS)

Slovacek, R.E.; Cramer, D.S.; Bean, E.B.; Hockenbury, R.W.; Valentine, J.R.; Block, R.C.

1975-01-01

The 238 U (n,f) cross section has been measured from 3 eV to about 30 keV with the lead slowing down spectrometer at the RPI Linac. Four fission ionization chambers containing a total of about 0.8 gm of 238 U (4.1 ppm 235 U) were used for the measurements. The fission widths of the 6.67, 20.9, and the 36.8 eV resonances were measured as (10 +- 1), (58 +- 9), and (12 +- 2) nanoelectron-volts respectively. The fission cross section integrated over the two subthreshold groups at 720 and 1210 eV and the average fission cross section from 10 to 30 keV are in agreement with a previous time of flight measurement. The fission width at 6.67 eV is 20 times smaller than an upper limit set by the only reported measurement in this energy region; the fission widths obtained in the present investigation are consistent with the (30 +- 50) nanoelectronvolt average width previously obtained for the resonances between 37 and 327 eV in a time of flight measurement using a nuclear device. From the measured fission widths, the 238 U thermal fission cross section was determined to be 2.7 +- 0.3 μ barns. The resonance fission integral was also obtained from the data as 1.33 +- 0.15 mbarns for 238 U. (4 figures, 4 tables) (U.S.)

Mutagenesis in yam, Discorea rotundata: Clonal evaluation of M{sub 1}V{sub 3} yam plants

Energy Technology Data Exchange (ETDEWEB)

Nwachukwu, E C; Mbanso, E N; Ene, L S.O. [Plant Breeding Div., National Root Crops Research Inst., Umudike, Umuahia, Abia State (Nigeria)

1997-12-01

Ten thousand plants of M{sub 1}V{sub 3} population of the white guinea yam, Dioscorea rotundata Poir, were evaluated. There was no consistent trends in variations in plant height, number of branches, branching heights and number of leaves of the treated tubers. However, plant height were lower in the irradiated than in the control. The coefficients of variations (C.V.) were higher in the irradiated than in the control populations, indicating wider variations in the former population. Based on yield performance, 110 tubers were selected for preliminary yield trials, Considering that diplontic selection may occur in the irradiated micro-tubers, the experiment will also be carried out using nodal cultures in vitro. (author). 2 tabs.

V S30, slope, H 800 and f 0: performance of various site-condition proxies in reducing ground-motion aleatory variability and predicting nonlinear site response

Science.gov (United States)

Derras, Boumédiène; Bard, Pierre-Yves; Cotton, Fabrice

2017-09-01

The aim of this paper is to investigate the ability of various site-condition proxies (SCPs) to reduce ground-motion aleatory variability and evaluate how SCPs capture nonlinearity site effects. The SCPs used here are time-averaged shear-wave velocity in the top 30 m ( V S30), the topographical slope (slope), the fundamental resonance frequency ( f 0) and the depth beyond which V s exceeds 800 m/s ( H 800). We considered first the performance of each SCP taken alone and then the combined performance of the 6 SCP pairs [ V S30- f 0], [ V S30- H 800], [ f 0-slope], [ H 800-slope], [ V S30-slope] and [ f 0- H 800]. This analysis is performed using a neural network approach including a random effect applied on a KiK-net subset for derivation of ground-motion prediction equations setting the relationship between various ground-motion parameters such as peak ground acceleration, peak ground velocity and pseudo-spectral acceleration PSA ( T), and M w, R JB, focal depth and SCPs. While the choice of SCP is found to have almost no impact on the median ground-motion prediction, it does impact the level of aleatory uncertainty. V S30 is found to perform the best of single proxies at short periods ( T < 0.6 s), while f 0 and H 800 perform better at longer periods; considering SCP pairs leads to significant improvements, with particular emphasis on [ V S30- H 800] and [ f 0-slope] pairs. The results also indicate significant nonlinearity on the site terms for soft sites and that the most relevant loading parameter for characterising nonlinear site response is the "stiff" spectral ordinate at the considered period.[Figure not available: see fulltext.

Preparation and biological evaluation of 18F-MPPF as a 5-HT1A imaging agent

International Nuclear Information System (INIS)

Wu Chunying; Lin Xiangtong; Zhang Zhengwei; Liu Ping; Xue Fangping; Lu Chunxiong; Zou Meifen; Chen Zhengping; Jiang Quanfu; Fu Ronggeng; Wang Songpei; Zhang Tongxing; Li Xiaomin; Zhu Junqing

2004-01-01

Objective: To develop and evaluate 4- 18 F-fluoro-N-2-[1-(2-methoxyphenyl)-1-piperazinyl] ethyl-N-2-pyridinyl-benzamide ( 18 F-MPPF) as a 5-hydroxytryptamine (5-HT 1A ) imaging agent. Methods: Nucleophilic substitution of fluoro replacement reaction was proceeding in dimethyl sulfoxide (DMSO) solution by oil-bath heating. Radiochemical purity (RCP) was determined by high pressure liquid chromatography (HPLC). Biological evaluations were performed in rats and mice. Results: RCP determined by HPLC was over 95% and were stable within 3 h. Biodistribution studies in rats showed that the initial uptake of 18 F-MPPF in the brain was high [(0.621±0.010)%ID/organ at 2 min]. The specific binding [(T/CB)-1] in hippocampus reaches its peak value of 2.70 at 30 min postinjection. (T/CB)-1 in hippocampus were significantly reduced to 0.89, 0.74 and 1.93 by pretreatment with 8-hydroxy-2-N, N-(di-n-propyl) aminotetralin (8-OH-DPAT), 4-(2'-methoxy-phenyl)-1-{2'-[n-(2 ) -pyridinyl]-cyclohexanecarboxamido}-ethyl piperazine (WAY100635) and spiperone at 30 min postinjection, respectively. The rat brain autoradiography and analysis showed that there was high 131 I-4-(2'-methoxy-phenyl)-1-{2'-[n-(2 ) -pyridinyl]-p-iodobenzamido}-ethyl piperazine (MPPI) uptake in hippocampus, the hippocampus/cerebellum ratio was significantly reduced from 13.98±0.87 to 1.96±0.46 by pretreatment with 8-OH-DPAT at 30 min postinjection. Conclusions: 18 F-MPPF can be specifically accumulated in hippocampus. It suggests that 18 F-MPPF might be a useful imaging agent for the studies of localization, function and modulation of 5-HT 1A receptor in the brain

Preliminary physical design of 7 MeV proton RFQ for the accelerator driven-energy system

International Nuclear Information System (INIS)

Luo Zihua

2000-01-01

The preliminary physical design of 7 MeV proton RFQ for the ADS (Accelerator Driven-energy System) is briefly described. The design features and the basic parameters and the design version of the RFQ are discussed. The matches between IS and RFQ and between RFQ and CCDTL/DTL are also discussed. The ideas of research for the RFQ are presented

18F-NaF PET/CT for the evaluation of temporomandibular joint disorder.

Science.gov (United States)

Suh, M S; Park, S H; Kim, Y-K; Yun, P-Y; Lee, W W

2018-04-01

To investigate the usefulness of a quantitative parameter (maximum standardised uptake value [SUVmax]) of 18 F-sodium fluoride (NaF) positron-emission tomography (PET)/computed tomography (CT) for the evaluation of temporomandibular joint (TMJ) disorder (TMD). Seventy-six TMD patients (male: female=14:62, age=40.3±17.1 years, bilateral: unilateral=40:36) with 152 TMJs were enrolled. The 18 F-NaF PET/CT parameter (SUVmax) was compared with the presence of TMJ arthralgia (arthralgic=86, non-arthralgic=66) and clinical subtypes based on the Research Diagnostic Criteria for TMD Axis I (TMD osteoarthritis=49, non-TMD osteoarthritis=67, and asymptomatic TMJ=36). Splint therapy was applied to 48 patients for 6 months without considering 18 F-NaF PET/CT findings. Post-splint therapy 18 F-NaF PET/CT was performed in 32 patients and clinical responses to the therapy were classified into improvement (n=33), no change (n=10), or aggravation (n=7) for 50 TMJs excluding asymptomatic TMJs (n=14). SUVmax was significantly greater in arthralgic TMJs than in non-arthralgic TMJs (6.62±3.56 versus 4.32±1.53, pchange in SUVmax was observed in improved (from 6.16±2.68 to 6.09±2.60, p=0.4915) and unchanged (from 6.46±4.19 to 6.77±4.32, p=0.3223) TMJs. 18 F-NaF PET/CT is a useful imaging tool for TMD evaluation because SUVmax showed a fair diagnostic performance for arthralgic TMJ and TMD osteoarthritis, and a correlation with the therapeutic response. Copyright © 2017 The Royal College of Radiologists. Published by Elsevier Ltd. All rights reserved.

18F-FDG PET in Parkinsonism: Differential Diagnosis and Evaluation of Cognitive Impairment.

Science.gov (United States)

Meyer, Philipp T; Frings, Lars; Rücker, Gerta; Hellwig, Sabine

2017-12-01

Accurate differential diagnosis of parkinsonism is of paramount therapeutic and prognostic importance. In addition, with the development of invasive therapies and novel disease-specific therapies, strategies for patient enrichment in trial populations are of growing importance. Imaging disease-specific patterns of regional glucose metabolism with PET and 18 F-FDG allows for a highly accurate distinction between Parkinson disease (PD) and atypical parkinsonian syndromes, including multiple-system atrophy, progressive supranuclear palsy, and corticobasal degeneration. On the basis of a preliminary metaanalysis of currently available studies with inclusion of multiple disease groups, we estimated that the diagnostic sensitivity and specificity for visual PET readings supported by voxel-based statistical analyses for diagnosis of atypical parkinsonian syndromes are 91.4% and 90.6%, respectively. The diagnostic specificity of 18 F-FDG PET for diagnosing multiple-system atrophy, progressive supranuclear palsy, and corticobasal degeneration was consistently shown to be high (>90%), whereas sensitivity was more variable (>75%). It is increasingly acknowledged that cognitive impairment represents a major challenge in PD, with mild cognitive impairment being a prodromal stage of PD with dementia (PDD). In line with clinical and neuropsychologic studies, recent PET studies demonstrated that posterior cortical dysfunction in nondemented PD patients precedes cognitive decline and the development of PDD by several years. Taken together, the current literature underscores the utility of 18 F-FDG PET for diagnostic evaluation of parkinsonism and the promising role of 18 F-FDG PET for assessment and risk stratification of cognitive impairment in PD. © 2017 by the Society of Nuclear Medicine and Molecular Imaging.

Protracted Clonal Trajectory of a JAK2 V617F-Positive Myeloproliferative Neoplasm Developing during Long-Term Remission from Acute Myeloid Leukemia

Directory of Open Access Journals (Sweden)

Stephen E. Langabeer

2018-01-01

Full Text Available Although transformation of the myeloproliferative neoplasms (MPNs to acute myeloid leukemia (AML is well documented, development of an MPN in patients previously treated for, and in remission from, AML is exceedingly rare. A case is described in which a patient was successfully treated for AML and in whom a JAK2 V617F-positive MPN was diagnosed after seven years in remission. Retrospective evaluation of the JAK2 V617F detected a low allele burden at AML diagnosis and following one course of induction chemotherapy. This putative chemoresistant clone subsequently expanded over the intervening seven years, resulting in a hematologically overt MPN. As AML relapse has not occurred, the MPN may have arose in a separate initiating cell from that of the AML. Alternatively, both malignancies possibly evolved from a common precursor defined by a predisposition mutation with divergent evolution into MPN through acquisition of the JAK2 V617F and AML through acquisition of different mutations. This case emphasizes the protracted time frame from acquisition of a disease-driving mutation to overt MPN and further underscores the clonal complexity in MPN evolution.

75 FR 70861 - Airworthiness Directives; Fokker Services B.V. Model F.28 Mark 0100, 1000, 2000, 3000, and 4000...

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2010-11-19

....V. Model F.28 Mark 0100, 1000, 2000, 3000, and 4000 Airplanes AGENCY: Federal Aviation... Fokker Services B.V. Model F28 Mark 1000, 2000, 3000, and 4000 airplanes, all serial numbers, equipped... Instructions of Fokker Service Bulletin SBF28-28-052, dated April 20, 2010 (for Model F28 Mark 1000, 2000, 3000...

Development of an atomic clock on an atom chip: Optimisation of the coherence time and preliminary characterisation

International Nuclear Information System (INIS)

Lacroute, Clement

2010-01-01

We describe the construction and preliminary characterization of an atomic clock on an atom chip. A sample of magnetically trapped 87 Rb atoms is cooled below 1 μK, close to Bose- Einstein condensation temperature. The trapped states |F = 1; m F = -1> and |F = 2;m F = 1> define our two-photon clock transition. Atoms are trapped around a field B0 = 3.23 G, where the clock frequency is first-order insensitive to magnetic field fluctuations. We have designed an atom chip that includes a microwave coplanar waveguide which drives the 6.835 GHz transition. The whole clock cycle is performed in the vicinity of the chip surface, making the physics package compact (5 cm) 3 . We first describe the experimental setup of the clock, and the optical bench that has been developed and characterized during this thesis. We then give the results obtained for atom cooling, which led to obtaining a 3 10 4 atoms Bose-Einstein condensate. We finally present the results obtained by Ramsey spectroscopy of the clock transition. We measure coherence times exceeding 10 seconds with our setup, dominated by atom losses. A preliminary measurement shows that the clock relative frequency stability is of 6 10 -12 at 1 s, limited by technical noise. Our goal is to reach a stability in the low 10 -13 at 1 s, i.e. better than commercial clocks and competitive with today's best compact clocks. (author)

fMRI-vs-MEG evaluation of post-stroke interhemispheric asymmetries in primary sensorimotor hand areas.

Science.gov (United States)

Altamura, Claudia; Torquati, Kahtya; Zappasodi, Filippo; Ferretti, Antonio; Pizzella, Vittorio; Tibuzzi, Francesco; Vernieri, Fabrizio; Pasqualetti, Patrizio; Landi, Doriana; Del Gratta, Cosimo; Romani, Gian-Luca; Maria Rossini, Paolo; Tecchio, Franca

2007-04-01

Growing evidence emphasizes a positive role of brain ipsilesional (IL) reorganization in stroke patients with partial recovery. Ten patients affected by a monohemispheric stroke in the middle cerebral artery territory underwent functional magnetic resonance (fMRI) and magnetoencephalography (MEG) evaluation of the primary sensory (S1) activation via the same paradigm (median nerve galvanic stimulation). Four patients did not present S1 fMRI activation [Rossini, P.M., Altamura, C., Ferretti, A., Vernieri, F., Zappasodi, F., Caulo, M., Pizzella, V., Del Gratta, C., Romani, G.L., Tecchio, F., 2004. Does cerebrovascular disease affect the coupling between neuronal activity and local haemodynamics? Brain 127, 99-110], although inclusion criteria required bilateral identifiable MEG responses. Mean Euclidean distance between fMRI and MEG S1 activation Talairach coordinates was 10.1+/-2.9 mm, with a 3D intra-class correlation (ICC) coefficient of 0.986. Interhemispheric asymmetries, evaluated by an MEG procedure independent of Talairach transformation, were outside or at the boundaries of reference ranges in 6 patients. In 3 of them, the IL activation presented medial or lateral shift with respect to the omega-shaped post-rolandic area while in the other 3, IL areas were outside the peri-rolandic region. In conclusion, despite dissociated intensity, the MEG and fMRI activations displayed good spatial consistency in stroke patients, thus confirming excessive interhemispheric asymmetries as a suitable indicator of unusual recruitments in the ipsilesional hemisphere, within or outside the peri-rolandic region.

76 FR 6541 - Airworthiness Directives; Fokker Services B.V. Model F.28 Mark 0100, 1000, 2000, 3000, and 4000...

Science.gov (United States)

2011-02-07

... Airworthiness Directives; Fokker Services B.V. Model F.28 Mark 0100, 1000, 2000, 3000, and 4000 Airplanes AGENCY.... Applicability (c) This AD applies to Fokker Services B.V. Model F.28 Mark 1000, 2000, 3000, and 4000 airplanes... April 20, 2010 (for Model F.28 Mark 1000, 2000, 3000, and 4000 airplanes); or SBF100-28-063, dated April...

{sup 18}F{sub F}DG PET in evaluating malignancies compared with CT, MRI and pathology in 58 cases

Energy Technology Data Exchange (ETDEWEB)

Li, L. [Air Force General Hospital, Beijing (Switzerland)

2000-07-01

diagnosed as intracranial metastasis on CT and MRI, had been corrected as infarction based on glucose metabolism. Other one lung abscess, shown dense FDG uptake on PET, was confirmed by typical manifestation on high resolution CT. Our preliminary clinical application of {sup 18}F-FDG PET imaging in oncology has demonstrated powerful advantage in characterizing tumor lesions, differentiating recurrent disease from treatment effects, staging tumors, evaluating the extent of disease, and monitoring therapy. But {sup 18}F-FDG PET still cant replace CT or MRI in malignance diagnosis. It will be used to approach and hopefully answer the difficult diagnosis problems which both CT and MRI can't adequately address at present Ifs clear that combined imaging analysis of PET and CT or MRI, which can give both and functional or metabolism changes of diseases, have an important and growing role in oncology clinical practice.

Tensile properties of V-Cr-Ti alloys after exposure in hydrogen-containing environments

International Nuclear Information System (INIS)

Natesan, K.; Soppet, W.K.

1998-01-01

A systematic study has been initiated at Argonne National Laboratory to evaluate the performance of several V-Cr-Ti alloys after exposure to environments containing hydrogen at various partial pressures. The goal is to correlate the chemistry of the exposure environment with hydrogen uptake in the samples and its influence on the microstructure and tensile properties of the alloys. At present, the principal effort has focused on the V-4Cr-4Ti alloy of heat identified as BL-71; however other alloys (V-5Cr-5Ti alloy of heats BL-63, and T87, plus V-4Cr-4Ti alloy from General Atomics [GA]) are also being evaluated. Other variables of interest are the effect of initial grain size on the tensile behavior of the alloys. Experiments conducted on specimens of various V-Cr-Ti alloys exposed to pH 2 levels of 0.01 and 3 x 10 -6 torr showed negligible effect of H 2 on either maximum engineering stress or uniform and total elongation. However, uniform and total elongation decreased substantially when the alloys were exposed to 1.0 torr H 2 pressure. Preliminary data from sequential exposures of the materials to low-pO 2 and several low-pH 2 environments did not reveal an adverse effect on the maximum engineering stress or on uniform and total elongation. Further, tests in H 2 environments on specimens annealed at different temperatures showed that grain-size variation by a factor of ∼2 had little or no effect on tensile properties

Validation of foF2 and TEC Modeling During Geomagnetic Disturbed Times: Preliminary Outcomes of International Forum for Space Weather Modeling Capabilities Assessment

Science.gov (United States)

Shim, J. S.; Tsagouri, I.; Goncharenko, L. P.; Kuznetsova, M. M.

2017-12-01

To address challenges of assessment of space weather modeling capabilities, the CCMC (Community Coordinated Modeling Center) is leading the newly established "International Forum for Space Weather Modeling Capabilities Assessment." This presentation will focus on preliminary outcomes of the International Forum on validation of modeled foF2 and TEC during geomagnetic storms. We investigate the ionospheric response to 2013 Mar. geomagnetic storm event using ionosonde and GPS TEC observations in North American and European sectors. To quantify storm impacts on foF2 and TEC, we first quantify quiet-time variations of foF2 and TEC (e.g., the median and the average of the five quietest days for the 30 days during quiet conditions). It appears that the quiet time variation of foF2 and TEC are about 10% and 20-30%, respectively. Therefore, to quantify storm impact, we focus on foF2 and TEC changes during the storm main phase larger than 20% and 50%, respectively, compared to 30-day median. We find that in European sector, both foF2 and TEC response to the storm are mainly positive phase with foF2 increase of up to 100% and TEC increase of 150%. In North America sector, however, foF2 shows negative effects (up to about 50% decrease), while TEC shows positive response (the largest increase is about 200%). To assess modeling capability of reproducing the changes of foF2 and TEC due to the storm, we use various model simulations, which are obtained from empirical, physics-based, and data assimilation models. The performance of each model depends on the selected metrics, therefore, only one metrics is not enough to evaluate the models' predictive capabilities in capturing the storm impact. The performance of the model also varies with latitude and longitude.

Presurgical evaluation of pediatric epilepsy patients prior to hemispherotomy: the prognostic value of 18F-FDG PET.

Science.gov (United States)

Traub-Weidinger, Tatjana; Weidinger, Philip; Gröppel, Gundrun; Karanikas, Georgios; Wadsak, Wolfgang; Kasprian, Gregor; Dorfer, Christian; Dressler, Anastasia; Muehlebner, Angelika; Hacker, Marcus; Czech, Thomas; Feucht, Martha

2016-12-01

OBJECTIVE The objective of this study was to investigate whether fluorine-18 fluorodeoxyglucose PET ( 18 F-FDG PET) can help to predict seizure outcome after hemispherotomy and therefore may be useful in decision making and patient selection. METHODS Children and adolescents less than 18 years of age who underwent 18 F-FDG PET studies during presurgical evaluation prior to hemispherotomy and had follow-up data of at least 12 months after surgery were included. Seizure outcome was classified according to the recommendations of the International League Against Epilepsy. PET data were reevaluated by two specialists in nuclear medicine blinded to clinical data and to MRI. MRI studies were also reinterpreted visually by an experienced neuroradiologist blinded to clinical data and PET findings. RESULTS Thirty-five patients (17 girls) with a median age of 5 years (range 0.4-17.8 years) were evaluable. Of the 35 patients, 91.4% were seizure free after surgery, including 100% of those with unilateral 18 F-FDG-PET hypometabolism compared with only 75% of those with bilateral hypometabolism. With respect to MRI, seizure freedom after surgery was observed in 96.4% of the patients with unilateral lesions compared with only 71.4% in those with bilateral MRI lesions. The best seizure outcomes were noted in patients with unilateral findings in both PET and MRI (100% seizure freedom) whereas only 50% of those with bilateral findings in both imaging techniques were seizure free. Furthermore, 100% of the patients with unilateral PET hypometabolism and bilateral MRI findings were also seizure free, but only 87.5% of those with bilateral PET hypometabolism and unilateral MRI findings. CONCLUSIONS According to these results, candidate selection for hemispherotomy can be optimized by the use of 18 F-FDG PET as part of a multimodal presurgical evaluation program, especially in patients with inconsistent (bilateral) MRI findings.

Preliminary Geological Map of the Fortuna Tessera (V-2) Quadrangle, Venus

Science.gov (United States)

Ivanov, M. A.; Head, J. W.

2009-01-01

The Fortuna Tessera quadrangle (50-75 N, 0-60 E) is a large region of tessera [1] that includes the major portion of Fortuna and Laima Tesserae [2]. Near the western edge of the map area, Fortuna Tessera is in contact with the highest moun-tain belt on Venus, Maxwell Montes. Deformational belts of Sigrun-Manto Fossae (extensional structures) and Au ra Dorsa (contractional structures) separate the tessera regions. Highly deformed terrains correspond to elevated regions and mildly deformed units are with low-lying areas. The sets of features within the V-2 quadrangle permit us to address the following important questions: (1) the timing and processes of crustal thickening/thinning, (2) the nature and origin of tesserae and deformation belts and their relation to crustal thickening processes, (3) the existence or absence of major evolutionary trends of volcanism and tectonics. The key feature in all of these problems is the regional sequence of events. Here we present description of units that occur in the V-2 quadrangle, their regional correlation chart (Fig. 1), and preliminary geological map of the region (Fig. 2).

Preliminary evaluation of a novel energy-resolved photon-counting gamma ray detector.

Science.gov (United States)

Meng, L-J; Tan, J W; Spartiotis, K; Schulman, T

2009-06-11

In this paper, we present the design and preliminary performance evaluation of a novel energy-resolved photon-counting (ERPC) detector for gamma ray imaging applications. The prototype ERPC detector has an active area of 4.4 cm × 4.4 cm, which is pixelated into 128 × 128 square pixels with a pitch size of 350 µm × 350µm. The current detector consists of multiple detector hybrids, each with a CdTe crystal of 1.1 cm × 2.2 cm × 1 mm, bump-bonded onto a custom-designed application-specific integrated circuit (ASIC). The ERPC ASIC has 2048 readout channels arranged in a 32 × 64 array. Each channel is equipped with pre- and shaping-amplifiers, a discriminator, peak/hold circuitry and an analog-to-digital converter (ADC) for digitizing the signal amplitude. In order to compensate for the pixel-to-pixel variation, two 8-bit digital-to-analog converters (DACs) are implemented into each channel for tuning the gain and offset. The ERPC detector is designed to offer a high spatial resolution, a wide dynamic range of 12-200 keV and a good energy resolution of 3-4 keV. The hybrid detector configuration provides a flexible detection area that can be easily tailored for different imaging applications. The intrinsic performance of a prototype ERPC detector was evaluated with various gamma ray sources, and the results are presented.

Association of TNF polymorphisms with JAK2 (V617F) myeloproliferative neoplasms in Brazilian patients.

Science.gov (United States)

Macedo, Luciana Conci; de Cesare Quintero, Fernanda; Pagliari-E-Silva, Sara; Pagnano, Katia Borgia Barbosa; Rodrigues, Camila; de Alencar, Josiane Bazzo; Sell, Ana Maria; Visentainer, Jeane Eliete Laguila

2016-03-01

The classical chromosome Philadelphia-negative myeloproliferative neoplasms (MPNs) are a group of disorders that share clinical, hematological, and histological features. Proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) are elevated in patients with MPN. The aim of this study was to verify the association between the polymorphisms of TNF gene (-308G/A and -238 G/A) in BCR-ABL-negative MPN in our population. Blood samples obtained from MPN patients were genotyped for the JAK2V617F mutation and both TNF polymorphisms using PCR-RFLP. Thirty three (26.8%) patients with polycythemia vera (PV), 35 (28.7%) essential thrombocythemia (ET), 22 (17.7%) primary myelofibrosis (PMF), and 33 (26.8%) with unclassifiable MPN (MPNu) were included in the study. The JAK2 V617F mutation was detected in 94 (76.42%) patients. Were observed a significant increase on the frequency of the TNF-238 GA genotype in MPN patients compared to controls (OR=2.21, 95% CI=1.02-4.80, P<0.04). The distribution of the genotypes and allelic frequencies of TNF-308 was significantly different among the MPNs, JAK2V617F positive, PV and PMF, and controls. Our data has demonstrated that the polymorphisms on TNF-238 GA, TNF-308 GA were associated to MPN development in this population, triggered by JAK2 V617F mutation. Copyright © 2015. Published by Elsevier Inc.

A Low Cost C8051F006 SoC-Based Quasi-Static C-V Meter for Characterizing Semiconductor Devices

Directory of Open Access Journals (Sweden)

Khairurrijal Khairurrijal

2012-12-01

Full Text Available Based on a C8051F006 SoC (system on-a-chip, a simple and low cost quasi-static capacitance-voltage (C-V meter was designed and developed to obtain C-V characteristics of semiconductor devices. The developed C-V meter consists of a capacitance meter, a programmable voltage source, a C8051F006 SoC-based slave controller, and a personal computer (PC as a master controller. The communication between the master and slave controllers is facilitated by the RS 232 serial communication. The accuracy of the C-V meter was guaranteed by the calibration functions, which are employed by the program in the PC and obtained through the calibration processes of analog to digital converter (ADC, digital to analog converters (DACs of the C8051F006 SoC, and the programmable voltage source. Examining 33-pF and 1000-pF capacitors as well three different p-n junction diodes, it was found that the capacitances of common capacitors are in the range of specified values and typical C-V curves of p-n junction diodes are achieved.

[Evaluation and symptomatic treatment of surinfectious exacerbations of COPD: preliminary study of antibiotic treatment combined with fenspiride (Pneumorel 80mg) versus placebo].

Science.gov (United States)

Lirsac, B; Benezet, O; Dansin, E; Nouvet, G; Stach, B; Voisin, C

2000-02-01

Exacerbations of chronic obstructive pulmonary disease (COPD) have an inflammatory component in addition to the possible infectious component. The antiinflammatory properties of fenspiride (Pneumorel(R) 80 mg) should be evaluated in this frequent clinical situation. Assess the supplementary therapeutic benefit provided by fenspiride administered in combination with antibiotics in COPD patients presenting an episode of bronchial infection. A preliminary randomized placebo-controlled double-blind study was conduced in 7 centers. Patients under 80 years of age of both sexes were included. All patients had COPD and presented a bronchial infection defined as the presence of at least 2 of the 3 criteria defined by Anthonisen. Patients were randomly assigned to group F or group P. Group F received an antibiotic therapy from day 1 to day 11 plus fenspiride (3 x 80mg/d from day 0 to day 30). Group P received the same antibiotic therapy plus placebo. Amoxicillin 500mg plus clavulanic acid 125, 3 tablets/day, was administered in both groups. Thirty-nine patients were included (group F 19 patients, group P 20 patients; 6 women and 33 men; mean age 61.1 +/- 9.8 years). The 3 Anthonisen criteria were present in 79% and 75% of the patients in group F and P respectively (NS). On day 11, expectoration resolved in 39% and 32% (NS) and cough in 44% and 16% (NS) of the patients in groups F and P respectively. Lung auscultation returned to normal in 83% of the patients in group F compared with 47% in group P (p=0.05). A composite clinical score including expectoration cough and auscultation findings showed that 28% of the patients in group F were symptom-free on day 11 compared with 0% in group P (p=0.04). On day 30, the two groups were comparable. In this preliminary study of patients with COPD presenting a bronchial superinfection, there was a significant improvement in lung auscultation and in the composite clinical score in patients given fenspiride. Fenspiride was thus found to

Preliminary 19F-MRS Study of Tumor Cell Proliferation with 3′-deoxy-3′-fluorothymidine and Its Metabolite (FLT-MP

Directory of Open Access Journals (Sweden)

In Ok Ko

2017-01-01

Full Text Available The thymidine analogue 3′-deoxy-3′-[18F]fluorothymidine, or [18F]fluorothymidine ([18F]FLT, is used to measure tumor cell proliferation with positron emission tomography (PET imaging technology in nuclear medicine. FLT is phosphorylated by thymidine kinase 1 (TK1 and then trapped inside cells; it is not incorporated into DNA. Imaging with 18F-radiolabeled FLT is a noninvasive technique to visualize cellular proliferation in tumors. However, it is difficult to distinguish between [18F]FLT and its metabolites by PET imaging, and quantification has not been attempted using current imaging methods. In this study, we successfully acquired in vivo F19 spectra of natural or nonradioactive 3′-deoxy-3′-fluorothymidine ([19F]FLT and its monophosphate metabolite (FLT-MP in a tumor xenograft mouse model using 9.4T magnetic resonance imaging (MRI. This preliminary result demonstrates that 19F magnetic resonance spectroscopy (MRS with FLT is suitable for the in vivo assessment of tumor aggressiveness and for early prediction of treatment response.

Jak2 46/1 Haplotype Is Associated With Jak2 V617f--positive Myeloproliferative Neoplasms In Brazilian Patients.

OpenAIRE

Macedo, L C; Santos, B C; Pagliarini-e-Silva, S; Pagnano, K B B; Rodrigues, C; Quintero, F C; Ferreira, M E; Baraldi, E C; Ambrosio-Albuquerque, E P; Sell, A M; Visentainer, J E L

2016-01-01

This study aimed to verify the association between the JAK2 46/1 haplotype (V617F positive) and some hematological parameters in BCR-ABL-negative chronic myeloproliferative neoplasms (cMPNs) in our population. The blood samples obtained from the patients with cMPN were genotyped for the JAK2 V617F mutation and JAK2 rs10974944 SNP screening using a PCR-RFLP assay. The JAK2 V617F mutation was detected in 80.15% of patients. The G variant of rs10974944 was more frequent in all MPNs, especially t...

Role of TiF4 in Microleakage of Silorane and Methacrylate-based Composite Resins in Class V Cavities.

Science.gov (United States)

Koohpeima, Fatemeh; Sharafeddin, Farahnaz; Jowkar, Zahra; Ahmadzadeh, Samaneh; Mokhtari, Mohammad Javad; Azarian, Babak

2016-03-01

and physical properties of modern composites than earlier methacrylate-based composites, polymerization shrinkage has been remaining as one of the main shortcomings of them. Different methods, such as using new low shrinkage resin composites and different dentin pretreatments, have been suggested to overcome this problem. This study evaluated the effect of TiF4 as pretreatment on microleakage of class V tooth preparations restored with a nanocomposite and a silorane-based resin composite.

JAK2 V617F, MPL W515L and JAK2 Exon 12 Mutations in Chinese Patients with Primary Myelofibrosis.

Science.gov (United States)

Xia, Jun; Lu, Mi-Ze; Jiang, Yuan-Qiang; Yang, Guo-Hua; Zhuang, Yun; Sun, Hong-Li; Shen, Yun-Feng

2012-03-01

JAK2 V617F, MPL W515L and JAK2 exon 12 mutations are novel acquired mutations that induce constitutive cytokine-independent activation of the JAK-STAT pathway in myeloproliferative disorders (MPD). The discovery of these mutations provides novel mechanism for activation of signal transduction in hematopoietic malignancies. This research was to investigate their prevalence in Chinese patients with primary myelofibrosis (PMF). We introduced allele-specific PCR (AS-PCR) combined with sequence analysis to simultaneously screen JAK2 V617F, MPL W515L and JAK2 exon 12 mutations in 30 patients with PMF. Fifteen PMF patients (50.0%) carried JAK2 V617F mutation, and only two JAK2 V617F-negative patients (6.7%) harbored MPL W515L mutation. None had JAK2 exon 12 mutations. Furthermore, these three mutations were not detected in 50 healthy controls. MPL W515L and JAK2 V617F mutations existed in PMF patients but JAK2 exon 12 mutations not. JAK2 V617F and MPL W515L and mutations might contribute to the primary molecular pathogenesis in patients with PMF.

Evaluations of the 58Fe(n,γ)59Fe, and 54Fe(n,p)54Mn reactions for the ENDF/B-V dosimetry file

International Nuclear Information System (INIS)

Schenter, R.E.; Schmittroth, F.; Mann, F.M.

1979-10-01

A generalized least-squares adjustment procedure was used to evaluate two important dosimetry reactions for the ENDF/B-V files. Calculations for the cross section adjustments were made with the computer code FERRET, where input data included both integral and differential experimental data results. For the 54 Fe reaction, important ratio measurements were renormalized to ENDF/B-V evaluations of 235 U(n,f), 238 U(n,f), and 56 Fe(n,p). A priori curves which are required for the calculations were obtained using Hauser-Feshbach calculations from the codes NCAP ( 58 Fe) and HAUSER-5 ( 54 Fe). Covariance matrices were also calculated and are included in the evaluations. 8 figures, 1 table

Large-xF spin asymmetry in π0 production by 200-GeV polarized protons

International Nuclear Information System (INIS)

Adams, D.L.; Corcoran, M.D.; Cranshaw, J.; Nessi-Tedaldi, F.; Nessi, M.; Nguyen, C.; Roberts, J.B.; Skeens, J.; White, J.L.; Bystricky, J.; Lehar, F.; Lesquen, A. de; Cossairt, J.D.; Read, A.L.; En'yo, H.; Funahashi, H.; Goto, Y.; Imai, K.; Itow, Y.; Makino, S.; Masaike, A.; Miyake, K.; Nagamine, T.; Saito, N.; Yamashita, S.; Iwatani, K.; Krueger, K.W.; Kuroda, K.; Michalowicz, A.; Luehring, F.C.; Miller, D.H.; Pauletta, G.; Penzo, A.; Schiavon, P.; Zanetti, A.; Salvato, G.; Villari, A.; Takeutchi, F.; Tamura, N.; Tanaka, N.; Yoshida, T.

1992-01-01

The spin asymmetry A N for inclusive π 0 production by 200-GeV transversely-polarized protons on a liquid hydrogen target has been measured at Fermilab over a wide range of x F , with 0.5 T F >0.3, the asymmetry rises with increasing x F and reaches a value of A N =0.15±0.03 in the region 0.6 F <0.8. This result provides new input regarding the question of the internal spin structure of transversely-polarized protons. (orig.)

Evaluation of the PET/CT with F.D.G. in the masculine fertility evaluation; Evaluation de la TEP/TDM au FDG dans l'evaluation de la fertilite masculine

Energy Technology Data Exchange (ETDEWEB)

Dierickx, L.O.; Zerdoud, S.; Brillouet, S.; Filleron, T.; Caselles, O. [Institut Claudius-Regaud, 31 - Toulouse (France); Huyghe, E.; Courbon, F. [Centre hospitalier universitaire de Rangeuil, 31 - Toulouse (France); Plante, P. [Centre hospitalier universitaire Paule de Viguier, 31 - Toulouse (France); Bujan, L.

2010-07-01

Purpose: the contribution of a functional imaging examination is expected by the andrology and urology community in the situations of masculine infertility (especially after chemotherapy). The spermatogonia, responsible of cells populations renewal, catch the F.D.G. tracer through GLUT3 receptors. To date, PET/CT with F.D.G. has never been used for functional study of testes with andrology aim. The objective is to search an eventual correlation between the F.D.G. testes uptake and spermatology examination. Conclusions: This preliminary study shows that among men without any known testes pathology, there is a significant correlation between the F.D.G. testes uptake and the principal parameters of masculine fertility. If these results are confirmed, clinical applications could appear in the diagnosis and prognosis exploration of hypo fertility. (N.C.)

TcoF-DB v2: update of the database of human and mouse transcription co-factors and transcription factor interactions

KAUST Repository

Schmeier, Sebastian; Alam, Tanvir; Essack, Magbubah; Bajic, Vladimir B.

2016-01-01

Transcription factors (TFs) play a pivotal role in transcriptional regulation, making them crucial for cell survival and important biological functions. For the regulation of transcription, interactions of different regulatory proteins known as transcription co-factors (TcoFs) and TFs are essential in forming necessary protein complexes. Although TcoFs themselves do not bind DNA directly, their influence on transcriptional regulation and initiation, although indirect, has been shown to be significant, with the functionality of TFs strongly influenced by the presence of TcoFs. In the TcoF-DB v2 database, we collect information on TcoFs. In this article, we describe updates and improvements implemented in TcoF-DB v2. TcoF-DB v2 provides several new features that enables exploration of the roles of TcoFs. The content of the database has significantly expanded, and is enriched with information from Gene Ontology, biological pathways, diseases and molecular signatures. TcoF-DB v2 now includes many more TFs; has substantially increased the number of human TcoFs to 958, and now includes information on mouse (418 new TcoFs). TcoF-DB v2 enables the exploration of information on TcoFs and allows investigations into their influence on transcriptional regulation in humans and mice. TcoF-DB v2 can be accessed at http://tcofdb.org/.

TcoF-DB v2: update of the database of human and mouse transcription co-factors and transcription factor interactions

KAUST Repository

Schmeier, Sebastian

2016-10-17

Transcription factors (TFs) play a pivotal role in transcriptional regulation, making them crucial for cell survival and important biological functions. For the regulation of transcription, interactions of different regulatory proteins known as transcription co-factors (TcoFs) and TFs are essential in forming necessary protein complexes. Although TcoFs themselves do not bind DNA directly, their influence on transcriptional regulation and initiation, although indirect, has been shown to be significant, with the functionality of TFs strongly influenced by the presence of TcoFs. In the TcoF-DB v2 database, we collect information on TcoFs. In this article, we describe updates and improvements implemented in TcoF-DB v2. TcoF-DB v2 provides several new features that enables exploration of the roles of TcoFs. The content of the database has significantly expanded, and is enriched with information from Gene Ontology, biological pathways, diseases and molecular signatures. TcoF-DB v2 now includes many more TFs; has substantially increased the number of human TcoFs to 958, and now includes information on mouse (418 new TcoFs). TcoF-DB v2 enables the exploration of information on TcoFs and allows investigations into their influence on transcriptional regulation in humans and mice. TcoF-DB v2 can be accessed at http://tcofdb.org/.

Kas Euroopa integratsiooni areng on võimalik föderaliseerumisega ühel ajal? / Viljar Veebel, Ulrika Hurt

Index Scriptorium Estoniae

Veebel, Viljar, 1977-

2013-01-01

Analüüsitakse põhjuslikku seost föderaliseerumise, integratsiooniprotsessi, euroopastumise ja majanduskriisi võitmise vahel, kas föderaliseerumine on teostatav ja millised valikud vastaksid Eesti huvidele

Synthesis, Molecular Structure and Characterization of Allylic Derivatives of 6-Amino-3-methyl-1,2,4-triazolo[3,4-f][1,2,4]-triazin-8(7H-one

Directory of Open Access Journals (Sweden)

Gene-Hsiang Lee

2006-06-01

Full Text Available 1-Allyl- (2 and 7-allyl-6-amino-3-methyl-1,2,4-triazolo[3,4-f][1,2,4]triazin-8(7H-one (3 were obtained via the 18-crown-6-ether catalyzed room temperature reactionof 6-amino-3-methyl-1,2,4-triazolo[3,4-f][1,2,4]triazin-8(7H-one (1 with potassiumcarbonate and allyl bromide in dry acetone. The structures of these two derivatives wereverified by 2D-NMR measurements, including gHSQC and gHMBC measurements. Theminor compound 2 may possess aromatic character. A single crystal X-ray diffractionexperiment indicated that the major compound 3 crystallizes from dimethyl sulfoxide in themonoclinic space group P21/n and its molecular structure includes an attached dimethylsulfoxide molecule, resulting in the molecular formula C10H16N6O2S. Molecular structuresof 3 are linked by extensive intermolecular N-H···N hydrogen bonding [graph set C 1 (7]. 1Each molecule is attached to the dimethyl sulfoxide oxygen via N-H···O intermolecularhydrogen bonding. The structure is further stabilized by π-π stacking interactions.

Driver mutations (JAK2V617F, MPLW515L/K or CALR), pentraxin-3 and C-reactive protein in essential thrombocythemia and polycythemia vera.

Science.gov (United States)

Lussana, Federico; Carobbio, Alessandra; Salmoiraghi, Silvia; Guglielmelli, Paola; Vannucchi, Alessandro Maria; Bottazzi, Barbara; Leone, Roberto; Mantovani, Alberto; Barbui, Tiziano; Rambaldi, Alessandro

2017-02-22

The driver mutations JAK2V617F, MPLW515L/K and CALR influence disease phenotype of myeloproliferative neoplasms (MPNs) and might sustain a condition of chronic inflammation. Pentraxin 3 (PTX3) and high-sensitivity C-reactive protein (hs-CRP) are inflammatory biomarkers potentially useful for refining prognostic classification of MPNs. We evaluated 305 with essential thrombocythemia (ET) and 172 polycythemia vera (PV) patients diagnosed according to the 2016 WHO criteria and with full molecular characterization for driver mutations. PTX3 levels were significantly increased in carriers of homozygous JAK2V617F mutation compared to all the other genotypes and triple negative ET patients, while hs-CRP levels were independent of the mutational profile. The risk of haematological evolution and death from any cause was about 2- and 1.5-fold increased in individuals with high PTX-3 levels, while the thrombosis rate tended to be lower. High hs-CRP levels were associated with risk of haematological evolution, death and also major thrombosis. After sequential adjustment for potential confounders (age, gender, diagnosis and treatments) and the presence of JAK2V617F homozygous status, high hs-CRP levels remained significant for all outcomes, while JAK2V617F homozygous status as well as treatments were the factors independently accounting for adverse outcomes among patients with high PTX3 levels. These results provide evidence that JAK2V617F mutation influences MPN-associated inflammation with a strong correlation between allele burden and PTX3 levels. Plasma levels of hs-CRP and PTX3 might be of prognostic value for patients with ET and PV, but their validation in future prospective studies is needed.

Measurement of K-electron capture probability in the decay of 87Y

International Nuclear Information System (INIS)

Prasad, N.V.S.V.; Murty, G.S.K.; Rao, M.V.S.C.; Sastry, D.L.

1993-01-01

The K-electron capture probability for the 1/2 - to 3/2 - transition in the decay of 87 Y to the 873.0 keV level in the daughter 87 Sr was measured for the first time using an x-γ summing method. The experimental P K value was found to be 0.911 ± 0.047, in agreement with the theoretical value of 0.878. (author)

Hadronic production of J/[psi] at large [chi][sub F] in 800 GeV p+Cu and p+Be collisions

Energy Technology Data Exchange (ETDEWEB)

Kowitt, M.S.

1992-12-01

The differential cross-section d[sigma]/dx[sub F] for J/[psi] production in 800 GeV proton-nucleus collisions has been measured in the kinematic range 0.30 [le] x[sub F] [le] 0.95 and x[sub F] [lt] p[perpendicular] [lt] 5 GeV through the decay mode J/[psi] [yields] [mu][sup +][mu][sup [minus

Sensorless Sinusoidal Drives for Fan and Pump Motors by V/f Control

Science.gov (United States)

Kiuchi, Mitsuyuki; Ohnishi, Tokuo

This paper proposes sensorless sinusoidal driving methods of permanent magnet synchronous motors for fans and pumps by V/f control. The proposed methods are simple methods that control the motor peak current constant by voltage or frequency control, and are characterized by DC link current detection using a single shunt resistor at carrier wave signal bottom timing. As a result of the dumping factor from square torque load characteristics of fan and pump motors, it is possible to control stable starting and stable steady state by V/f control. In general, pressure losses as a result of the fluid pass of fan and pump systems are nearly constant; therefore, the flow rate and motor torque are determined by revolutions. Accordingly, high efficiency driving is possible by setting corresponding currents to q-axis currents (torque currents) at target revolutions. Because of the simple current detection and motor control methods, the proposed methods are optimum for fan and pump motor driving systems of home appliances.

The role of the F.D.G.-PET in the evaluation of efficacy of radiofrequency treatment of metastases

International Nuclear Information System (INIS)

Ungureanu, C.M.; Angoue, O.; Blagosklonov, O.; Zsigmond, R.; Boulahdour, H.; Kastler, B.

2009-01-01

Objectives Methods such as MRI and CT usually utilized in the assessment of the radiofrequency (RF) efficacy proved to be insufficient in the early detection of the residual tumors after RF treatment. In this study, we evaluated the effectiveness of utilizing the PET-CT in the follow-up of the RF treated patients. Methods PET-CT was performed before and after five RF treatments (three patients). Absence of focally increased F.D.G. uptake was considered complete efficacy, while the presence of focally increased F.D.G. uptake was considered incomplete efficacy. The efficacy was estimated on the PET-CT performed in the same day to three months after the RF treatment. Every RF administration was followed by at least two PET-CT exams. We used radiological evaluation as a control method. Results Complete efficacy was found for three of the five RF treatments and incomplete efficacy in two cases. The disease evolution confirmed the results obtained through PET-CT in 100% of the cases studied. Conclusion This preliminary study showed that the PET-CT has the potential of evaluating the efficacy of the RF treatment. Our findings showed that the PET-CT may very early evidence the presence of residual tumors. (authors)

New results on multi-muon production in 250 GeV μ+ Fe interactions

International Nuclear Information System (INIS)

Best, C.H.

1981-05-01

Preliminary results on the contribution to the nucleon structure function F 2 from charm particle production are presented. The data consist of dimuon and trimuon events from a high statistics experiment for a beam energy of 250 GeV. Exotic trimuon events have been observed and are compared to predictions from BETABETA and tautau production and decay. (author)

Purification, crystallization and preliminary X-ray analysis of the IgV domain of human nectin-4.

Science.gov (United States)

Xu, Xiang; Zhang, Xiaoai; Lu, Guangwen; Cai, Yongping

2012-08-01

Nectin-4 belongs to a family of immunoglobulin-like cell adhesion molecules and is highly expressed in cancer cells. Recently, nectin-4 was found to be a receptor of measles virus and the IgV domain sustains strong binding to measles virus H protein. In this study, the successful expression and purification of human nectin-4 V domain (nectin-4v) is reported. The purified protein was crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to 1.8 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 Å, β = 94.7°. Preliminary analysis of the diffraction data was also performed.

Comparative study of different methodologies to detect the JAK2 V617F mutation in chronic BCR-ABL1 negative myeloproliferative neoplasms

Directory of Open Access Journals (Sweden)

Alline Didone

2016-04-01

Full Text Available Objectives: A mutation in the JAK2 gene, V617F, has been identified in several BCR-ABL1 negative myeloproliferative neoplasms (MPN: polycythemia vera (PV, essential thrombocythemia (ET, and primary myelofibrosis (PMF. Defining the presence or absence of this mutation is an essential part of clinical diagnostic algorithms and patient management. Here, we aimed to evaluate the performance of three PCR-based assays: Amplification Refractory Mutation System (ARMS, High-Resolution Melting analysis (HRM, and Sanger direct sequencing, and compare their results with those obtained by a PCR restriction fragment polymorphism assay (PCR-RFLP. Design and methods: We used blood samples from 136 patients (PV=20; PMF=20; ET=28, and other MPN suspected cases=68. Results: Comparable results were observed among the four assays in patients with PV, PMF, and MPN suspected cases. In patients with a diagnosis of ET, the JAK2 V617F mutation was detected in 67.8% of them by the PCR-ARMS and PCR-HRM assay and in 64% of them by the conventional Sanger sequence approach. The PCR-ARMS and PCR-HRM assays were 100% concordant. With these tests, only one of the 20 patients with ET and one of the three patients with clinically suspected MPN gave different results compared with those obtained by the PCR-RFLP. Conclusions: Our results have demonstrated that the PCR-ARMS and PCR-HRM assays could detect the JAK2 V617F mutation effectively in MPN patients, but PCR-HRM assays are rapid and the most cost-effective procedures. Keywords: Myeloproliferative, JAK2 V617F, Mutation, Wild type, Screening

A Preliminary Rubric Design to Evaluate Mixed Methods Research

Science.gov (United States)

Burrows, Timothy J.

2013-01-01

With the increase in frequency of the use of mixed methods, both in research publications and in externally funded grants there are increasing calls for a set of standards to assess the quality of mixed methods research. The purpose of this mixed methods study was to conduct a multi-phase analysis to create a preliminary rubric to evaluate mixed…

Connecticut Transit (CTTRANSIT) Fuel Cell Transit Bus: Preliminary Evaluation Results

Energy Technology Data Exchange (ETDEWEB)

Chandler, K.; Eudy, L.

2008-10-01

This report provides preliminary results from a National Renewable Energy Laboratory evaluation of a protoptye fuel cell transit bus operating at Connecticut Transit in Hartford. Included are descriptions of the planned fuel cell bus demonstration and equipment; early results and agency experience are also provided.

B-physics from lattice QCD...with a twist

CERN Document Server

Carrasco, N.; Frezzotti, R.; Gimenez, V.; Herdoiza, G.; Lubicz, V.; Martinelli, G.; Michael, C.; Palao, D.; Rossi, G.C.; Sanfilippo, F.; Shindler, A.; Simula, S.; Tarantino, C.

2012-01-01

We present a precise lattice QCD determination of the b-quark mass, of the B and Bs decay constants and first results for the B-meson bag parameters. For our computation we employ the so-called ratio method and our results benefit from the use of improved interpolating operators for the B-mesons. QCD calculations are performed with Nf = 2 dynamical light-quarks at four values of the lattice spacing and the results are extrapolated to the continuum limit. The preliminary results are mb(mb) = 4.35(12) GeV for the MSbar b-quark mass, fBs = 234(6) MeV and fB = 197(10) MeV for the B-meson decay constants, BBs(mb) = 0.90(5) and BB(mb) = 0.87(5) for the B-meson bag parameters.

Medium energy elementary particle physics: Technical progress report, 11/1/86 thru 5/28/87

International Nuclear Information System (INIS)

1987-01-01

We have searched for spontaneous conversion of muonium (M identical to μ + e - ) to antimuonium (anti M identical to μ - e + ) at LAMPF. When μ + of 10 MeV/c pass through an Al foil (0.2 mg/cm 2 ), M atoms with kinetic energies of 1 to 20 keV are formed by e - -capture. They pass a drift space of 342 cm, of which 206 cm is magnetically shielded to ≤20 mG, and are stopped on a 1 μm thick Bi target, which was evaporated onto a 2 mil mylar backing and coated with 75 A of MgO. The impact of M or anti M atoms on the target liberates about 5 secondary electrons each with kinetic energies of a few eV. These are electrostatically collected and guided onto a microchannel plate detector, using electric fields of about 100 V/cm. We have counted M at 1 to 20 keV with 50% efficiency using this 0.4 m 2 area active target. If an oscillation of M to anti M has occurred, stopping an anti M atom will entail formation of a Biμ - atom and emission of its characteristic muonic x rays. The crystal box detector then detects the coincident Lα and Kα x rays at 4% efficiency. The anti M event signature was defined as a triple coincidence of a Biμ - Lα x ray, a Kα x ray, and a count in the M(anti M) secondary emission counter. No statistically significant signal was seen and our preliminary data analysis yields an upper limit of G/sub M anti M/ ≤ 8 G/sub F/ (95% C.L.) on the coupling constant of a four-Fermion contact interaction. The current published limit is G/sub M anti M/ ≤ 20 G/sub F/. A conversion of M to anti M is forbidden in the standard model, since it would violate additive lepton flavor conservation. However, an alternative theory based on a lef-tright symmetric model allows the M → anti M coupling at G/sub M anti M/ - 10 G/sub F/. 8 refs., 4 figs

Detection of CALR and MPL Mutations in Low Allelic Burden JAK2 V617F Essential Thrombocythemia.

Science.gov (United States)

Usseglio, Fabrice; Beaufils, Nathalie; Calleja, Anne; Raynaud, Sophie; Gabert, Jean

2017-01-01

Myeloproliferative neoplasms are clonal hematopoietic stem cell disorders characterized by aberrant proliferation and an increased tendency toward leukemic transformation. The genes JAK2, MPL, and CALR are frequently altered in these syndromes, and their mutations are often a strong argument for diagnosis. We analyzed the mutational profiles of these three genes in a cohort of 164 suspected myeloproliferative neoplasms. JAK2 V617F mutation was detected by real-time PCR, whereas high-resolution melting analysis followed by Sanger sequencing were used for searching for mutations in JAK2 exon 12, CALR, and MPL. JAK2 V617F mutation was associated with CALR (n = 4) and MPL (n = 4) mutations in 8 of 103 essential thrombocytosis patients. These cases were harboring a JAK2 V617F allelic burden of MPL genes in myeloproliferative neoplasms and suggest that CALR and MPL should be analyzed not only in JAK2-negative patients but also in low V617F mutation patients. Follow-up of these double-mutation cases will be important for determining whether this group of patients presents particular evolution or complications. Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

Preliminary evaluation of microstructure and mechanical properties on low activation ferritic steels

International Nuclear Information System (INIS)

Hsu, C.Y.; Lechtenberg, T.A.

1985-01-01

Radioactive waste disposal has become a primary concern for the selection of materials for the structural components for fusion reactors. One way to minimize this potential environmental problem is to use structural materials in which the induced radioactivity decays quickly to levels that allow for near-surface disposal under 10CFR61 rules. The primary objective of this work is to develop low activation ferritic steels that exhibit mechanical and physical properties approximately equivalent to the HT-9 and 9Cr-1Mo steels, but which only contain elements that would permit near-surface disposal under 10CFR61 after exposure to fusion neutrons. A preliminary evaluation of the microstructure and mechanical properties of a 9Cr-2.5W-0.3V-0.15C (GA3X) low activation ferritic steel has been performed. An optimum heat treatment condition has been defined for GA3X steel. The properties and microstructure of the quenched and tempered specimens were characterized via hardness measurement and optical metallographic observation. The hot-microhardness and ductility parameter measurements were used to estimate the tensile properties at elevated temperatures. The estimated tensile strengths of GA3X steel at elevated temperatures are comparable to both 9Cr-1Mo and the modified 9Cr-1Mo steels. These preliminary results are encouraging in that they suggest that suitable low activation alloys can be successfully produced in this ferritic alloy class

Hanford Waste Vitrification Plant capacity increase options

International Nuclear Information System (INIS)

Larson, D.E.

1996-04-01

Studies are being conducted by the Hanford Waste Vitrification Plant (HWVP) Project on ways to increase the waste processing capacity within the current Vitrification Building structural design. The Phase 1 study on remote systems concepts identification and extent of capacity increase was completed. The study concluded that the HWVP capacity could be increased to four times the current capacity with minor design adjustments to the fixed facility design, and the required design changes would not impact the current footprint of the vitrification building. A further increase in production capacity may be achievable but would require some technology development, verification testing, and a more systematic and extensive engineering evaluation. The primary changes included a single advance melter with a higher capacity, new evaporative feed tank, offgas quench collection tank, ejector venturi scrubbers, and additional inner canister closure station,a smear test station, a new close- coupled analytical facility, waste hold capacity of 400,000 gallon, the ability to concentrate out-of-plant HWVP feed to 90 g/L waste oxide concentration, and limited changes to the current base slab construction package

One-step radiosynthesis of {sup 18}F-AlF-NOTA-RGD{sub 2} for tumor angiogenesis PET imaging

Energy Technology Data Exchange (ETDEWEB)

Liu, Shuanglong; Liu, Hongguang; Xu, Yingding; Cheng, Zhen [Stanford University, Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Bio-X Program, Department of Radiology, Stanford, CA (United States); Jiang, Han [Stanford University, Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Bio-X Program, Department of Radiology, Stanford, CA (United States); Institute of Nuclear Medicine and Molecular Imaging, and the Second Affiliated Hospital of Zhejiang University School of Medicine, Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Department of Nuclear Medicine, Medical PET Center, Hangzhou, Zhejiang (China); Zhang, Hong [Institute of Nuclear Medicine and Molecular Imaging, and the Second Affiliated Hospital of Zhejiang University School of Medicine, Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Department of Nuclear Medicine, Medical PET Center, Hangzhou, Zhejiang (China)

2011-09-15

One of the major obstacles of the clinical translation of {sup 18}F-labeled arginine-glycine-aspartic acid (RGD) peptides has been the laborious multistep radiosynthesis. In order to facilitate the application of RGD-based positron emission tomography (PET) probes in the clinical setting we investigated in this study the feasibility of using the chelation reaction between Al{sup 18}F and a macrocyclic chelator-conjugated dimeric RGD peptide as a simple one-step {sup 18}F labeling strategy for development of a PET probe for tumor angiogenesis imaging. Dimeric cyclic peptide E[c(RGDyK)]{sub 2} (RGD{sub 2}) was first conjugated with a macrocyclic chelator, 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA), and the resulting bioconjugate NOTA-RGD{sub 2} was then radiofluorinated via Al{sup 18}F intermediate to synthesize {sup 18}F-AlF-NOTA-RGD{sub 2}. Integrin binding affinities of the peptides were assessed by a U87MG cell-based receptor binding assay using {sup 125}I-echistatin as the radioligand. The tumor targeting efficacy and in vivo profile of {sup 18}F-AlF-NOTA-RGD{sub 2} were further evaluated in a subcutaneous U87MG glioblastoma xenograft model by microPET and biodistribution. NOTA-RGD{sub 2} was successfully {sup 18}F-fluorinated with good yield within 40 min using the Al{sup 18}F intermediate. The IC{sub 50} of {sup 19}F-AlF-NOTA-RGD{sub 2} was determined to be 46 {+-} 4.4 nM. Quantitative microPET studies demonstrated that {sup 18}F-AlF-NOTA-RGD{sub 2} showed high tumor uptake, fast clearance from the body, and good tumor to normal organ ratios. NOTA-RGD{sub 2} bioconjugate has been successfully prepared and labeled with Al{sup 18}F in one single step of radiosynthesis. The favorable in vivo performance and the short radiosynthetic route of {sup 18}F-AlF-NOTA-RGD{sub 2} warrant further optimization of the probe and the radiofluorination strategy to accelerate the clinical translation of {sup 18}F-labeled RGD peptides. (orig.)

A preliminary comparison of F region plasma drifts and E region irregularity drifts in the auroral zone

International Nuclear Information System (INIS)

Ecklund, W.L.; Balsley, B.B.; Carter, D.A.

1977-01-01

During several days in April--May 1976 the Chatanika, Alaska, incoherent scatter radar and a temporary Doppler auroral radar located at Aniak, Alaska, were directed toward ionospheric volumes along a common magnetic field line in order to compare E region and F region drifts and associated electric fields. The Chatanika radar measured F region plasma drifts via the incoherent scatter technique, while the Aniak radar measured the drifts of E region irregularities (i.e., the radar aurora). The radar geometry was arranged so that both radars measured approximately the same velocity component of a magnetically westward or eastward motion. Preliminary data show good agreement between the drift velocity components measured by the two techniques during most of the experimental period. This result indicates that relatively modest auroral radar systems may be used, with some qualifications, to determine auroral electric fields

JAK2V617F expression in mice amplifies early hematopoietic cells and gives them a competitive advantage that is hampered by IFNα.

Science.gov (United States)

Hasan, Salma; Lacout, Catherine; Marty, Caroline; Cuingnet, Marie; Solary, Eric; Vainchenker, William; Villeval, Jean-Luc

2013-08-22

The acquired gain-of-function V617F mutation in the Janus Kinase 2 (JAK2(V617F)) is the main mutation involved in BCR/ABL-negative myeloproliferative neoplasms (MPNs), but its effect on hematopoietic stem cells as a driver of disease emergence has been questioned. Therefore, we reinvestigated the role of endogenous expression of JAK2(V617F) on early steps of hematopoiesis as well as the effect of interferon-α (IFNα), which may target the JAK2(V617F) clone in humans by using knock-in mice with conditional expression of JAK2(V617F) in hematopoietic cells. These mice develop a MPN mimicking polycythemia vera with large amplification of myeloid mature and precursor cells, displaying erythroid endogenous growth and progressing to myelofibrosis. Interestingly, early hematopoietic compartments [Lin-, LSK, and SLAM (LSK/CD48-/CD150+)] increased with the age. Competitive repopulation assays demonstrated disease appearance and progressive overgrowth of myeloid, Lin-, LSK, and SLAM cells, but not lymphocytes, from a low number of engrafted JAK2(V617F) SLAM cells. Finally, IFNα treatment prevented disease development by specifically inhibiting JAK2(V617F) cells at an early stage of differentiation and eradicating disease-initiating cells. This study shows that JAK2(V617F) in mice amplifies not only late but also early hematopoietic cells, giving them a proliferative advantage through high cell cycling and low apoptosis that may sustain MPN emergence but is lost upon IFNα treatment.

Preliminary studies with [18F]haloperidol: a radioligand for in vivo studies of the dopamine receptors

International Nuclear Information System (INIS)

Tewson, T.J.; Raichle, M.E.; Welch, M.J.

1980-01-01

The authors report a synthesis of [ 18 F]haloperidol of sufficiently high specific activity to permit the mapping of dopamine receptors in vivo in man using PET. The preliminary work with this radioligand in vivo in monkeys clearly suggests that haloperidol enters brain from blood by means of carrier-mediated, facilitated diffusion rather than simple diffusion. This rather surprising observation not only assumes special importance in the interpretation of in vivo pharmacokinetic data on dopamine receptors in man or animals but may also be important in considerations of the possible mode of action of this drug on the central nervous system. (Auth.)

Evidence for core-coupled states in 87Y from a 89Y(p, t)87Y and 88Sr(p, t)86Sr comparison

International Nuclear Information System (INIS)

Oelrich, I.C.; Krien, K.; DelVecchio, R.M.; Naumann, R.A.

1976-01-01

The 89 Y(p, t) 87 Y and 88 Sr(p, t) 86 Sr reactions were studied at 42 MeV proton energy, using a quadrupole-dipole-dipole-dipole spectograph. Comparison of excitation energies, (p, t) cross section strengths and angular distribution shapes indicates that basis features of the core-coupling model apply to these nuclei. However, mixing of single particle states with the core-coupled states is evident. The (p, t) cross-section strength summed over the 87 Y multiplet is found with few exceptions to be nearly a constant multiple of the (p, t) strength of the associated 86 Sr state

Preventing structural degradation from Na3V2(PO4)3 to V2(PO4)3: F-doped Na3V2(PO4)3/C cathode composite with stable lifetime for sodium ion batteries

Science.gov (United States)

Chen, Yanjun; Xu, Youlong; Sun, Xiaofei; Zhang, Baofeng; He, Shengnan; Li, Long; Wang, Chao

2018-02-01

A prospective NASICON-type F-doped Na3V2(PO4)2.93F0.07/C (F-0.07-NVP/C) composite is synthesized by a solid-state reaction method. F-doping can restrain the structural degradation from Na3V2(PO4)3 to V2(PO4)3 and enhance the structural stability. Meanwhile, it can decrease the particle size to diminish the pathway of Na+ diffusion, which can increase ionic conductivity efficiently. The kinetic behavior is significantly improved and it is beneficial to reinforcing the electrochemical performance of F-doping composites. Compared with Undoped-NVP/C sample, F-0.07-NVP/C composite delivers a 113 mAh g-1 discharge capacity at 10 mA g-1, which is very close to the theoretical capacity (117 mAh g-1). As for cycle performance, a reversible capacity of 97.8 mAh g-1 can be obtained and it retains 86% capacity after 1000 cycles at 200 mA g-1. F-0.07-NVP/C composite presents the highest DNa+ (2.62 × 10-15 cm2s-1), two orders of magnitude higher than the undoped sample (4.8 × 10-17 cm2s-1). This outstanding electrochemical performance is ascribed to the synergetic effect from improved kinetic behavior and enhanced structural stability due to F-doping. Hence, the F-doped composite would be a promising cathode material in SIB for energy storage and conversion.

Development of a BaF2 scintillation spectrometer for evaluation of photon energy spectra in workplaces around nuclear facilities

International Nuclear Information System (INIS)

Urabe, Itsumasa; Yoshimoto, Taka-aki; Kobayashi, Katsuhei; Akiyoshi, Tsunekazu; Tsujimoto, Tadashi; Nakashima, Yoshiyuki; Oda, Keiji.

1997-01-01

A BaF 2 scintillation spectrometer has been constructed for the determination of photon energy spectra in workplaces around nuclear facilities. Energy absorption spectra by the BaF 2 detector were calculated with the EGS4 Monte Carlo code in the energy region from 0.1 to 100 MeV and a response matrix of the spectrometer was obtained from the energy absorption spectra, of which the energy resolutions were modified to fit to the experimental results. With the irradiation experiments using neutron-capture gamma rays and those from radioactive sources, it became clear that photon energy spectra can be evaluated within an error of about 10% in the energy region 0.1 MeV to a few tens of megaelectronvolts. (author)

Purification, crystallization and preliminary X-ray analysis of the IgV domain of human nectin-4

International Nuclear Information System (INIS)

Xu, Xiang; Zhang, Xiaoai; Lu, Guangwen; Cai, Yongping

2012-01-01

Nectin-4 belongs to a family of immunoglobulin-like cell adhesion molecules and is highly expressed in cancer cells. Recently, nectin-4 was found to be a receptor of measles virus and the IgV domain sustains strong binding to measles virus H protein. In this study, the successful expression and purification of human nectin-4 V domain (nectin-4v) is reported Nectin-4 belongs to a family of immunoglobulin-like cell adhesion molecules and is highly expressed in cancer cells. Recently, nectin-4 was found to be a receptor of measles virus and the IgV domain sustains strong binding to measles virus H protein. In this study, the successful expression and purification of human nectin-4 V domain (nectin-4v) is reported. The purified protein was crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to 1.8 Å resolution and belonged to space group P2 1 , with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 Å, β = 94.7°. Preliminary analysis of the diffraction data was also performed

High resolution measurement of the 231Pa(n,f) cross section from 0.4 eV to 12 MeV

International Nuclear Information System (INIS)

Plattard, S.; Auchampaugh, G.F.; de Saussure, G.

1980-01-01

The 231 Pa(n,f) reaction was studied to shed light on the existence of a shallow third minimum in the 232 Pa fission barrier. Results are plotted along with data points derived from ENDF/B-V. The occurrence of a pure vibrational state at E/sub n/ = 156.7 keV (3 + ) together with a nearby state of opposite parity favors the evidence for a third asymmetrically deformed minimum in the 232 Pa fission barrier. 2 figures

[Expression of JAK2V617F and MPLW515L/K mutation in 30 suspected cases of early myeloproliferative disorders].

Science.gov (United States)

Fan, Zheng; Zhang, Ri; Shen, Yi-Min; Fei, Hai-Rong; Zhu, Zi-Ling; Cen, Jian-Nong

2008-09-01

To investigate the prevalence of JAK2V617F and MPLW515L/K mutation in patients with slightly elevated platelets (BPC) or hemoglobin (Hb) not meeting the criteria of polycythemia vera (PV) or essential thrombocythemia (ET). Genomic DNA from bone marrow or blood mononuclear cells was screened with allele specific polymerase chain reaction (AS-PCR) for JAK2V617F and MPLW515L/K mutation. The history of thrombosis was assessed retrospectively by patients files. Of 30 patients, 14 (46.7%) were positive for the JAK2V617F mutation, none of them had the MPLW515L/ K. Five of these 14 patients had a history of thrombosis. Follow-up results were available in 22 patients. Among them, 12 patients with JAK2V617F mutation turned out to be MPD in 6-24 months; only 2 out of 10 patients without this mutation evolved to MPD. JAK2V617F mutation could be one of the diagnosis criteria of early MPD. No MPLW515L/K expression was found in early MPD.

Driver mutations (JAK2V617F, MPLW515L/K or CALR, pentraxin-3 and C-reactive protein in essential thrombocythemia and polycythemia vera

Directory of Open Access Journals (Sweden)

Federico Lussana

2017-02-01

Full Text Available Abstract Background The driver mutations JAK2V617F, MPLW515L/K and CALR influence disease phenotype of myeloproliferative neoplasms (MPNs and might sustain a condition of chronic inflammation. Pentraxin 3 (PTX3 and high-sensitivity C-reactive protein (hs-CRP are inflammatory biomarkers potentially useful for refining prognostic classification of MPNs. Methods We evaluated 305 with essential thrombocythemia (ET and 172 polycythemia vera (PV patients diagnosed according to the 2016 WHO criteria and with full molecular characterization for driver mutations. Results PTX3 levels were significantly increased in carriers of homozygous JAK2V617F mutation compared to all the other genotypes and triple negative ET patients, while hs-CRP levels were independent of the mutational profile. The risk of haematological evolution and death from any cause was about 2- and 1.5-fold increased in individuals with high PTX-3 levels, while the thrombosis rate tended to be lower. High hs-CRP levels were associated with risk of haematological evolution, death and also major thrombosis. After sequential adjustment for potential confounders (age, gender, diagnosis and treatments and the presence of JAK2V617F homozygous status, high hs-CRP levels remained significant for all outcomes, while JAK2V617F homozygous status as well as treatments were the factors independently accounting for adverse outcomes among patients with high PTX3 levels. Conclusions These results provide evidence that JAK2V617F mutation influences MPN-associated inflammation with a strong correlation between allele burden and PTX3 levels. Plasma levels of hs-CRP and PTX3 might be of prognostic value for patients with ET and PV, but their validation in future prospective studies is needed.

High prevalence of arterial thrombosis in JAK2 mutated essential thrombocythaemia: independence of the V617F allele burden

DEFF Research Database (Denmark)

Larsen, Thomas Stauffer; Pallisgaard, Niels; Møller, Michael Boe

2008-01-01

Approximately half of the patients with essential thrombocythaemia (ET) harbor the JAK2 V617F mutation. Despite a phenotypic mimicry of JAK2 V617F positive ET and polycythaemia vera (PV), the data on thromboembolic risk and correlation to JAK2 mutation status are ambiguous. On a strictly WHO defi...

Faktorer som förklarar miljövänligt beteende hos unga vuxna

OpenAIRE

Rikner, Amanda

2009-01-01

Människors miljörelaterade beteenden behöver förbättras. I studien undersöktes unga vuxna universitetsstuderandes syn på klimatproblematiken. En enkät delades ut som mätte miljövänligt beteende, ansvarskänsla, tillit till forskning, upplevelse av att kunna påverka och kunskap när det gäller klimatproblematiken. Deltagarna fick också beskriva anledningar som möjliggör eller förhindrar en förbättring av klimatproblematiken. Deltog gjorde 97 personer. Resultatet visade på signifikanta samband me...

Suicide intervention training evaluation: a preliminary report.

Science.gov (United States)

Tierney, R J

1994-01-01

To date, very little work has been done on evaluating training in suicide intervention. This study developed and piloted a comprehensive method for evaluating suicide intervention training by applying three studies of immediate training effects on (a) suicide intervention abilities, (b) attitudes to suicide and suicide intervention, and (c) knowledge about suicide. The focus of the evaluation was a broadly used 2-day suicide intervention training program. Changes in suicide intervention abilities were measured by the Suicide Intervention Response Inventory (SIRI) and by performance in simulated suicide intervention situations, scored with the Suicide Intervention Protocol (SIP). Subjects consisted of 19 workshop participants in a pre-post condition and 17 participants in a post-test only condition. Results indicated significant increases in skills in suicide intervention situations. No significant effects were noted on the SIRI. Results from the attitudes and knowledge studies were very preliminary. They are reported here so that others may become aware of the methodology being used and the status of evaluation of the target program. Implications for further research are discussed.

Evaluations on the R Wave in asymptomatic subjects: a preliminary ...

African Journals Online (AJOL)

This preliminary cross-sectional survey on the R wave of the 12-lead electrocardiogram (ECG) was undertaken to evaluate the normal R wave duration and amplitude of the young adult Nigerian from Jos; to ascertain significant correlation coefficients for R wave duration and amplitude, age and anthropometric ...

Measurement of the structure functions F 2 and xF 3 and comparison with QCD predictions including kinematical and dynamical higher twist effects

Science.gov (United States)

Varvell, K.; Cooper-Sarkar, A. M.; Parker, M. A.; Sansum, R. A.; Aderholz, M.; Armenise, N.; Baton, J. P.; Bullock, F. W.; Berggren, M.; Bertrand, D.; Brisson, V.; Burkot, W.; Calcchio, M.; Claytoh, E. F.; Coghen, T.; Erriquez, O.; Fitch, P. J.; Gerbier, G.; Guy, J.; Hulth, P. O.; Iaselli, G.; Jones, G. T.; Kasper, P.; Klein, H.; Kochowski, C.; Marage, P.; Mermikides, M.; Middleton, R. P.; Morrison, D. R. O.; Mobayyen, M. M.; Natali, S.; Neveu, M.; Nuzzo, S.; O'Neale, S. W.; Petiau, P.; Petrides, A.; Ruggieri, F.; Sacton, J.; Simopoulou, E.; Vallee, C.; Vayaki, A.; Venus, W. A.; Wachsmuth, H.; Wells, J.; Wittek, W.

1987-03-01

The isoscalar nucleon structure functions F 2( x, Q 2) and xF 3( x, Q 2) are measured in the range 0< Q 2<64 GeV2, 1.7< W 2<250 GeV2, x<0.7 using ν andbar v interactions on neon in BEBC. The data are used to evaluate possible higher twist contributions and to determine their impact on the evaluation of the QCD parameter Λ. In contrast to previous analyses reaching to such low W 2 values, it is found that a lowΛ _{overline {MS} } value in the neighbourhood of 100 MeV describes the data adequately and that the contribution of dynamical higher twist effects is small and negative.

fMRI orientation decoding in V1 does not require global maps or globally coherent orientation stimuli.

Science.gov (United States)

Alink, Arjen; Krugliak, Alexandra; Walther, Alexander; Kriegeskorte, Nikolaus

2013-01-01

The orientation of a large grating can be decoded from V1 functional magnetic resonance imaging (fMRI) data, even at low resolution (3-mm isotropic voxels). This finding has suggested that columnar-level neuronal information might be accessible to fMRI at 3T. However, orientation decodability might alternatively arise from global orientation-preference maps. Such global maps across V1 could result from bottom-up processing, if the preferences of V1 neurons were biased toward particular orientations (e.g., radial from fixation, or cardinal, i.e., vertical or horizontal). Global maps could also arise from local recurrent or top-down processing, reflecting pre-attentive perceptual grouping, attention spreading, or predictive coding of global form. Here we investigate whether fMRI orientation decoding with 2-mm voxels requires (a) globally coherent orientation stimuli and/or (b) global-scale patterns of V1 activity. We used opposite-orientation gratings (balanced about the cardinal orientations) and spirals (balanced about the radial orientation), along with novel patch-swapped variants of these stimuli. The two stimuli of a patch-swapped pair have opposite orientations everywhere (like their globally coherent parent stimuli). However, the two stimuli appear globally similar, a patchwork of opposite orientations. We find that all stimulus pairs are robustly decodable, demonstrating that fMRI orientation decoding does not require globally coherent orientation stimuli. Furthermore, decoding remained robust after spatial high-pass filtering for all stimuli, showing that fine-grained components of the fMRI patterns reflect visual orientations. Consistent with previous studies, we found evidence for global radial and vertical preference maps in V1. However, these were weak or absent for patch-swapped stimuli, suggesting that global preference maps depend on globally coherent orientations and might arise through recurrent or top-down processes related to the perception of

Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis

International Nuclear Information System (INIS)

Rathinaswamy, Priya; Pundle, Archana V.; Prabhune, Asmita A.; SivaRaman, Hepzibah; Brannigan, James A.; Dodson, Guy G.; Suresh, C. G.

2005-01-01

An unannotated protein reported from B. subtilis has been expressed in E. coli and identified as possessing penicillin V acylase activity. The crystallization and preliminary crystallographic analysis of this penicillin V acylase is presented. Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their β-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 4 M sodium formate in 100 mM Tris–HCl buffer pH 8.2. Diffraction data were collected under cryogenic conditions to a spacing of 2.5 Å. The crystals belonged to the orthorhombic space group C222 1 , with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 Å. The estimated Matthews coefficient was 3.23 Å 3 Da −1 , corresponding to 62% solvent content. The structure has been solved using molecular-replacement methods with B. sphaericus penicillin V acylase (PDB code 2pva) as the search model

A critical analysis of the majority judgment in F v Minister of Safety ...

African Journals Online (AJOL)

The majority judgment of Mogoeng CJ in F v Minister of Safety and Security 2012 1 ... norms now play an important role in deciding questions of vicarious liability. ... the conduct of the employee wrongdoer and the business of his employer as ...

G Protein-Coupled Receptor 87 (GPR87 Promotes Cell Proliferation in Human Bladder Cancer Cells

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Xia Zhang

2015-10-01

Full Text Available G protein-coupled receptor 87 (GPR87 is a newly deorphanized member of the cell surface molecule G protein-coupled receptor family. GPR signaling was shown to play a role in promotion of cell growth and survival, metastasis, and drug resistance. The overexpression of GPR87 has also been reported in many malignant tumors including bladder cancer. The aim of the present study is to examine the effect of silencing GPR87 expression with a replication-deficient recombinant adenoviral vector expressing short hairpin RNA targeting GPR87 (Ad-shGPR87 and to explore the underlying molecular mechanisms in bladder cancer cells. Six GPR87-expressing human bladder cancer cells, HT1197, HT1376, J82, RT112, TCCSUP and UMUC3, were used. Infection with Ad-shGPR87 effectively downregulated the GPR87 expression, and significantly reduced the percentage of viable cells in 4 of 6 cell lines as detected by an MTT assay. Significant inhibition on cell proliferation with Ad-shGPR87 was observed in the wild-type p53 bladder cancer cell lines (HT1197, RT112, TCCSUP and UMUC3, but not in the mutant p53 cells (HT1376 and J82. As represented by a wild-type p53 RT112 cell, Ad-shGPR87 infection significantly enhanced p53 and p21 expression and caused caspase-dependent apoptosis. Furthermore, the treatment with Ad-shGPR87 exerted a significant antitumor effect against the GPR87-expressing RT112 xenografts. GPR87 appeared to be a promising target for gene therapy, and Ad-shGPR87 had strong antitumor effects, specifically anti-proliferative and pro-apoptotic effects, against GPR87-expressing human bladder cancer cells.

Early evaluation of cerebral metabolic rate of glucose (CMRglu) with 18F-FDG PET/CT and clinical assessment in idiopathic normal pressure hydrocephalus (INPH) patients before and after ventricular shunt placement: preliminary experience

International Nuclear Information System (INIS)

Calcagni, Maria Lucia; Lavalle, Mariadea; Leccisotti, Lucia; Giordano, Alessandro; Mangiola, Annunziato; De Bonis, Pasquale; Anile, Carmelo; Indovina, Luca; Marra, Camillo; Pelliccioni, Armando

2012-01-01

We evaluated the relationships between the cerebral metabolic rate of glucose (CMRglu) measured by dynamic 18 F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) and the clinical and neuropsychological assessment before and after the surgical procedure in idiopathic normal pressure hydrocephalus (INPH) patients. Eleven selected INPH patients underwent clinical assessment (modified Rankin scale, Krauss scale, Larsson categorization system and Stein-Langfitt scale), cognitive evaluation (Mini-Mental State Examination, MMSE) and dynamic 18 F-FDG PET/CT scan 3 days before and 1 week after ventricular shunt placement. After shunting, the global CMRglu significantly increased (2.95 ± 0.44 vs 4.38 ± 0.68, p = 10 -7 ) in all INPH patients with a mean percentage value of 48.7%. After shunting, no significant change was found in the Evans ratio whereas a significant decrease in all clinical scale scores was observed. Only a slight reduction in the MMSE was found. After shunting, a significant correlation between the global CMRglu value and clinical assessment was found (R 2 = 0.75, p = 0.024); indeed all clinical scale scores varied (decreasing) and the CMRglu value also varied (increasing) in all INPH patients. Our preliminary data show that changes in the CMRglu are promptly reversible after surgery and that there is a relationship between the early metabolic changes and clinical symptoms, independently from the simultaneous changes in the ventricular size. The remarkable and prompt improvement in the global CMRglu and in symptoms may also have important implications for the current concept of ''neuronal plasticity'' and for the cells' reactivity in order to recover their metabolic function. (orig.)

Interactive Block Games for Assessing Children's Cognitive Skills: Design and Preliminary Evaluation

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Kiju Lee

2018-05-01

Full Text Available Background: This paper presents design and results from preliminary evaluation of Tangible Geometric Games (TAG-Games for cognitive assessment in young children. The TAG-Games technology employs a set of sensor-integrated cube blocks, called SIG-Blocks, and graphical user interfaces for test administration and real-time performance monitoring. TAG-Games were administered to children from 4 to 8 years of age for evaluating preliminary efficacy of this new technology-based approach.Methods: Five different sets of SIG-Blocks comprised of geometric shapes, segmented human faces, segmented animal faces, emoticons, and colors, were used for three types of TAG-Games, including Assembly, Shape Matching, and Sequence Memory. Computational task difficulty measures were defined for each game and used to generate items with varying difficulty. For preliminary evaluation, TAG-Games were tested on 40 children. To explore the clinical utility of the information assessed by TAG-Games, three subtests of the age-appropriate Wechsler tests (i.e., Block Design, Matrix Reasoning, and Picture Concept were also administered.Results: Internal consistency of TAG-Games was evaluated by the split-half reliability test. Weak to moderate correlations between Assembly and Block Design, Shape Matching and Matrix Reasoning, and Sequence Memory and Picture Concept were found. The computational measure of task complexity for each TAG-Game showed a significant correlation with participants' performance. In addition, age-correlations on TAG-Game scores were found, implying its potential use for assessing children's cognitive skills autonomously.

Las habilidades motoras en niños víctimas de maltrato físico

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Eulalia María Amador Rodero

2013-01-01

Full Text Available Objetivo: Aplicar un programa de habilidades motoras en niños víctimas de maltrato físico. Materiales y métodos: Se trata de un estudio cuasiexperimental en el que se aplica el programa de Estimulación de Habilidades Motoras formulado por el grupo POYMOCOR de la Universidad Libre a una población diana conformada por 16 niños víctimas de maltrato físico en la ciudad de Barranquilla (Colombia; los cuales cumplieron con los criterios de inclusión: haber sido víctimas de maltrato físico y no padecer de alteraciones musculoesquéliticas de origen congénito. La participación de los niños fue voluntaria, pre- via autorización de de la ONG que tiene bajo su cuidado a los niños víctimas de maltrato. El estudio fue autorizado por el Centro de Investigación de la Universidad Libre. Para el análisis de la información se utilizó el programa SPSS para estimar las frecuencias de las variables sociodemográficas y las medias y desviaciones estándar de cada subfactor de la Batería Psicomotriz (BPM de Vitor Da Fonseca, utilizada como instrumento de medición en este proyecto. Las diferencias significativas entre las medias de las puntuaciones de cada subfactor resultado de la evaluación antes y después de la intervención se establecie- ron mediante el test T de Students. Resultados: evidenciaron que el programa de estimulación es efectivo para mejorar las habilidades motoras en niños víctimas de maltrato fisico. Conclusiones: Se concluye que el maltrato físico deja secuelas que interfieren con la ad- quisición y desarrollo de habilidades motoras, pero los programas de estimulación, en este caso el formulado por el grupo investigador, son efectivos para que los niños recuperen sus habilidades y destrezas motoras.

Measurement of the inelastic branch of the stellar $^{14}$O($\\alpha$, p)$^{17}$F reaction occurring in the explosive burning in Novae and X-ray busters

CERN Document Server

He, J J; Hass, M; Warr, N; Raabe, R; Neyskens, P; Aliotta, M; Robinson, A P; Murphy, A St J; Davinson, T; Buescler, J; Wenander, F; Woods, P J; Jenkins, D G; Clement, E; Kumar, V; van der Walle, J

2010-01-01

The inelastic component of the key astrophysical resonance (1(-), E-x=6.15 MeV) in the O-14(alpha,p)F-17 reaction has been studied by using the resonant scattering of F-17+p. The experiment was done at REX-ISOLDE CERN with the Miniball setup. The thick target method in inverse kinematics was utilized in the present experiment where a 44.2 MeV F-17 beam bombarded a similar to 40 mu m thick (CH2)(n) target. The inelastic scattering protons in coincidence with the de-excited 495 keV gamma rays have been clearly seen and they are from the inelastic branch to the first excited state in F-17 following decay of the 1(-) resonance in Ne-18. Some preliminary results are reported.

Measurement of the inelastic branch of the stellar 14O(α,p)17F reaction occurring in the explosive burning in Novae and X-ray bursters

International Nuclear Information System (INIS)

He, J.J.; Woods, P.J.; Davinson, T.; Aliotta, M.; Buescler, J.; Clement, E.; Delahaye, P.; Hass, M.; Jenkins, D.G.; Kumar, V.; Murphy, A.St.J.; Neyskens, P.; Raabe, R.; Robinson, A.P.; Walle, J. van der; Warr, N.; Wenander, F.

2010-01-01

The inelastic component of the key astrophysical resonance (1 - , E x =6.15 MeV) in the 14 O(α,p) 17 F reaction has been studied by using the resonant scattering of 17 F+p. The experiment was done at REX-ISOLDE CERN with the Miniball setup. The thick target method in inverse kinematics was utilized in the present experiment where a 44.2 MeV 17 F beam bombarded a∼40μm thick (CH 2 ) n target. The inelastic scattering protons in coincidence with the de-excited 495 keV γ rays have been clearly seen and they are from the inelastic branch to the first excited state in 17 F following decay of the 1 - resonance in 18 Ne. Some preliminary results are reported.

Aceptabilidad del castigo físico en la crianza de los niños en personas que fueron víctimas de violencia física en la niñez en Perú

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Alejandra Burela

Full Text Available Objetivos. El estudio evaluó la asociación entre haber sido víctima de violencia física en la niñez y la aceptabilidad, en etapas posteriores de vida, hacia el uso del castigo físico en la crianza de los niños. Materiales y métodos. Se realizó un análisis secundario de un estudio sobre violencia en 6399 personas mayores de 14 años residentes de las ciudades de Lima, Callao, Maynas, Arequipa, Cusco, Trujillo y Huamanga. Se usó modelos de regresión logística univariados y multivariados para estimar asociaciones estadísticas. Resultados. La aceptabilidad del uso del castigo físico en la crianza de niños es mayor en personas con el antecedente de ser víctimas de violencia física durante la niñez en comparación con las no victimizadas (OR=1,8; IC 95%: 1,5-2,1; p<0,001 ajustando por potenciales variables de confusión. Conclusiones. Las personas expuestas a violencia física durante la niñez tienen más riesgo de aceptar o justificar la misma durante la adultez, lo que podría contribuir a mantener esta práctica de crianza de una generación a la siguiente. Iniciativas dirigidas a prevenir el uso del castigo físico en la crianza de niños se deben implementar para reducir la tendencia a reproducir el ejercicio de violencia en personas victimizadas.

Comparisons of [18F]-1-deoxy-1-fluoro-scyllo-inositol with [18F]-FDG for PET imaging of inflammation, breast and brain cancer xenografts in athymic mice

International Nuclear Information System (INIS)

McLarty, Kristin; Moran, Matthew D.; Scollard, Deborah A.; Chan, Conrad; Sabha, Nesrin; Mukherjee, Joydeep; Guha, Abhijit; McLaurin, JoAnne; Nitz, Mark; Houle, Sylvain; Wilson, Alan A.; Reilly, Raymond M.; Vasdev, Neil

2011-01-01

Introduction: The aim of the study was to evaluate the uptake of [ 18 F]-1-deoxy-1-fluoro-scyllo-inositol ([ 18 F]-scyllo-inositol) in human breast cancer (BC) and glioma xenografts, as well as in inflammatory tissue, in immunocompromised mice. Studies of [ 18 F]-2-fluoro-2-deoxy-D-glucose ([ 18 F]-FDG) under the same conditions were also performed. Methods: Radiosynthesis of [ 18 F]-scyllo-inositol was automated using a commercial synthesis module. Tumour, inflammation and normal tissue uptakes were evaluated by biodistribution studies and positron emission tomography (PET) imaging using [ 18 F]-scyllo-inositol and [ 18 F]-FDG in mice bearing subcutaneous MDA-MB-231, MCF-7 and MDA-MB-361 human BC xenografts, intracranial U-87 MG glioma xenografts and turpentine-induced inflammation. Results: The radiosynthesis of [ 18 F]-scyllo-inositol was automated with good radiochemical yields (24.6%±3.3%, uncorrected for decay, 65±2 min, n=5) and high specific activities (≥195 GBq/μmol at end of synthesis). Uptake of [ 18 F]-scyllo-inositol was greatest in MDA-MB-231 BC tumours and was comparable to that of [ 18 F]-FDG (4.6±0.5 vs. 5.5±2.1 %ID/g, respectively; P=.40), but was marginally lower in MDA-MB-361 and MCF-7 xenografts. Uptake of [ 18 F]-scyllo-inositol in inflammation was lower than [ 18 F]-FDG. While uptake of [ 18 F]-scyllo-inositol in intracranial U-87 MG xenografts was significantly lower than [ 18 F]-FDG, the tumour-to-brain ratio was significantly higher (10.6±2.5 vs. 2.1±0.6; P=.001). Conclusions: Consistent with biodistribution studies, uptake of [ 18 F]-scyllo-inositol was successfully visualized by PET imaging in human BC and glioma xenografts, with lower accumulation in inflammatory tissue than [ 18 F]-FDG. The tumour-to-brain ratio of [ 18 F]-scyllo-inositol was also significantly higher than that of [ 18 F]-FDG for visualizing intracranial glioma xenografts in NOD SCID mice, giving a better contrast. -- Graphical Abstract: Display Omitted

Evaluation of σ-1 receptor radioligand 18F-FTC-146 in rats and squirrel monkeys using PET

DEFF Research Database (Denmark)

James, Michelle L; Shen, Bin; Nielsen, Carsten Haagen

2014-01-01

. Pretreatment with known S1R compounds, haloperidol, or BD1047, before radioligand administration, significantly attenuated (18)F-FTC-146 accumulation in all rat brain regions by approximately 85% (P ...-FTC-146 was observed in rat plasma. Preliminary monkey PET/MRI studies demonstrated specific accumulation of (18)F-FTC-146 in the brain (mainly in cortical structures, cerebellum, and vermis) that could be attenuated by pretreatment with haloperidol. HPLC of monkey plasma suggested radioligand metabolism...

18F-FDOPA PET/CT-Guided Radiofrequency Ablation of Liver Metastases from Neuroendocrine Tumours: Technical Note on a Preliminary Experience

Energy Technology Data Exchange (ETDEWEB)

Cazzato, Roberto Luigi, E-mail: gigicazzato@hotmail.it; Garnon, Julien, E-mail: juleiengarnon@gmail.com [Nouvel Hôpital Civil (Hôpitaux Universitaires de Strasbourg, HUS), Department of Interventional Radiology (France); Ramamurthy, Nitin, E-mail: nitin-ramamurthy@hotmail.com [Norfolk and Norwich University Hospital, Department of Radiology (United Kingdom); Tsoumakidou, Georgia, E-mail: georgia.tsoumakidou@chru-strasbourg.fr [Nouvel Hôpital Civil (Hôpitaux Universitaires de Strasbourg, HUS), Department of Interventional Radiology (France); Imperiale, Alessio, E-mail: alessio.imperiale@chru-strasbourg.fr; Namer, Izzie Jacques, E-mail: izzie.jacques.namer@chru-strasbourg.fr [Hôpital de Hautepierre (Hôpitaux Universitaires de Strasbourg, HUS), Department of Biophysics and Nuclear Medicine (France); Bachellier, Philippe, E-mail: philippe.bachellier@chru-strasbourg.fr [Hôpital de Hautepierre (Hôpitaux Universitaires de Strasbourg, HUS), Hepato-Pancreato-Biliary Surgery and Liver Transplantation (France); Caudrelier, Jean, E-mail: jean.caudrelier@chru-strasbourg.fr; Rao, Pramod, E-mail: pramodrao@me.com; Koch, Guillaume, E-mail: guillaume.koch@chru-strasbourg.fr; Gangi, Afshin, E-mail: gangi@unistra.fr [Nouvel Hôpital Civil (Hôpitaux Universitaires de Strasbourg, HUS), Department of Interventional Radiology (France)

2016-09-15

AimTo review our preliminary experience with 6-l-18F-fluorodihydroxyphenylalanine (18F-FDOPA) PET/CT-guided radiofrequency ablation (RFA) of liver metastases from neuroendocrine tumours (NETs).Materials and MethodsThree patients (mean age 51.3 years; range 43–56) with gastro-entero pancreatic NET (GEP-NET) liver metastases underwent 18F-FDOPA PET/CT-guided RFA. Patients were referred with oligometastatic hepatic-confined disease (1–6 metastases; <3 cm) on 18F-FDOPA PET/CT; poor lesion visualisation on US, CT, and MR; and ongoing symptoms. Procedures were performed in an interventional PET/CT scanner under general anaesthesia using a split-dose protocol. Lesion characteristics, procedural duration and technical success (accurate probe placement and post-procedural ablation-zone photopaenia), complications, patient and operator dose, and clinical outcomes were evaluated.ResultsThirteen liver metastases (mean size 11.4 mm, range 8–16) were treated in three patients (two presented with “carcinoid syndrome”). Technical success was 100 % with a mean procedural duration of 173.3 min (range 90–210) and no immediate complications. Mean patient dose was 2844 mGy·cm (range 2104–3686). Operator and radiographer doses were acceptable other than the operator’s right hand in the first case (149 µSv); this normalised in the second case. There was no local tumour or extra-hepatic disease progression at mid-term follow-up (mean 12.6 months; range 6–20); however, two cases progressed with new liver metastases at different sites. There was 100 % clinical success (n = 2) in resolving carcinoid syndrome symptoms.Conclusion18F-FDOPA PET/CT-guided RFA appears technically feasible, safe, and effective in patients with GEP-NETs and low-burden hepatic metastases. Further prospective studies are required to elucidate its precise role in tailored multimodality management of GEP-NET liver metastases.

Cosmology based on f(R) gravity admits 1 eV sterile neutrinos.

Science.gov (United States)

Motohashi, Hayato; Starobinsky, Alexei A; Yokoyama, Jun'ichi

2013-03-22

It is shown that the tension between recent neutrino oscillation experiments, favoring sterile neutrinos with masses of the order of 1 eV, and cosmological data which impose stringent constraints on neutrino masses from the free streaming suppression of density fluctuations, can be resolved in models of the present accelerated expansion of the Universe based on f(R) gravity.

Strategy for product composition control in the Hanford Waste Vitrification Plant

International Nuclear Information System (INIS)

Bryan, M.F.; Piepel, G.F.

1996-03-01

The Hanford Waste Vitrification Plant (HWVP) will immobilize transuranic and high-level radioactive waste in borosilicate glass. The major objective of the Process/Product Model Development (PPMD) cost account of the Pacific Northwest Laboratory HWVP Technology Development (PHTD) Project is the development of a system for guiding control of feed slurry composition (which affects glass properties) and for checking and documenting product quality. This document lays out the broad structure of HWVP's product composition control system, discusses five major algorithms and technical issues relevant to this system, and sketches the path of development and testing

[RITA combined with temozolomide inhibits the proliferation of human glioblastoma U87 cells].

Science.gov (United States)

He, Xiao-Yan; Feng, Xiao-Li; Song, Xin-Pei; Zeng, Huan-Chao; Cao, Zhong-Xu; Xiao, Wei-Wei; Zhang, Bao; Wu, Qing-Hua

2016-10-20

To observe the effect of RITA, a small molecule that targets p53, combined with temozolomide (TMZ) on proliferation, colony formation and apoptosis of human glioblastoma U87 cells and explore the underlying mechanism. Cultured U87 cells were treated with RITA (1, 5, 10, 20 µmol/L), TMZ, or RITA+TMZ (half dose) for 24, 48 or 72 h. MTS assay were used to detect the cell proliferation, and the cell proliferation rate and inhibitory rate were calculated. The effect of combined treatments was evaluated by the q value. The expressions of p53, p21 and other apoptosis-associated genes were detected by qRT-PCR and Western blotting; cell apoptosis was assayed using flow cytometry with Annexin V/PI double staining; colony formation of the cells was detected with crystal violet staining. MTS assay showed that RITA at the 4 doses more potently inhibited U87 cell viability than TMZ at 72 h (P=0.000) with inhibitory rates of 25.94%-41.38% and 3.84%-8.20%, respectively. RITA combined with TMZ caused a more significant inhibition of U87 cells (29.21%-52.11%) than RITA (PRITA+TMZ for 48 h resulted in q values exceeding 1.2 and showed an obvious synergistic effect of the drugs. Both RITA and TMZ, especially the latter, significantly increased the expressions of p53, p21, puma, and other apoptosis-associated genes to accelerate apoptosis and inhibit the growth and colony formation of U87 cells, and the effect was more obvious with a combined treatment. RITA inhibits the growth of human glioblastoma cells and enhance their sensitivity to TMZ by up-regulating p53 expression, and when combined, RITA and TMZ show a synergistic effect to cause a stronger cell inhibition.

Preventative Vaccines for Zika Virus Outbreak: Preliminary Evaluation.

Science.gov (United States)

Kim, Eun; Erdos, Geza; Huang, Shaohua; Kenniston, Thomas; Falo, Louis D; Gambotto, Andrea

2016-11-01

Since it emerged in Brazil in May 2015, the mosquito-borne Zika virus (ZIKV) has raised global concern due to its association with a significant rise in the number of infants born with microcephaly and neurological disorders such as Guillain-Barré syndrome. We developed prototype subunit and adenoviral-based Zika vaccines encoding the extracellular portion of the ZIKV envelope gene (E) fused to the T4 fibritin foldon trimerization domain (Efl). The subunit vaccine was delivered intradermally through carboxymethyl cellulose microneedle array (MNA). The immunogenicity of these two vaccines, named Ad5.ZIKV-Efl and ZIKV-rEfl, was tested in C57BL/6 mice. Prime/boost immunization regimen was associated with induction of a ZIKV-specific antibody response, which provided neutralizing immunity. Moreover, protection was evaluated in seven-day-old pups after virulent ZIKV intraperitoneal challenge. Pups born to mice immunized with Ad5.ZIKV-Efl were all protected against lethal challenge infection without weight loss or neurological signs, while pups born to dams immunized with MNA-ZIKV-rEfl were partially protected (50%). No protection was seen in pups born to phosphate buffered saline-immunized mice. This study illustrates the preliminary efficacy of the E ZIKV antigen vaccination in controlling ZIKV infectivity, providing a promising candidate vaccine and antigen format for the prevention of Zika virus disease. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

PET imaging of α{sub 7} nicotinic acetylcholine receptors: a comparative study of [{sup 18}F]ASEM and [{sup 18}F]DBT-10 in nonhuman primates, and further evaluation of [{sup 18}F]ASEM in humans

Energy Technology Data Exchange (ETDEWEB)

Hillmer, Ansel T.; Li, Songye; Zheng, Ming-Qiang; Lin, Shu-fei; Nabulsi, Nabeel; Holden, Daniel; Pracitto, Richard; Labaree, David; Ropchan, Jim; Esterlis, Irina; Cosgrove, Kelly P.; Carson, Richard E.; Huang, Yiyun [Yale University, PET Center, New Haven, CT (United States); Scheunemann, Matthias; Teodoro, Rodrigo; Deuther-Conrad, Winnie; Brust, Peter [Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Leipzig (Germany)

2017-06-15

The α{sub 7} nicotinic acetylcholine receptor (nAChR) is implicated in many neuropsychiatric disorders, making it an important target for positron emission tomography (PET) imaging. The first aim of this work was to compare two α{sub 7} nAChRs PET radioligands, [{sup 18}F]ASEM 3-(1,4-diazabicyclo[3.2.2]nonan-4-yl)-6-([{sup 18}F]fluorodibenzo[b,d]thiophene 5,5-dioxide) and [{sup 18}F]DBT-10 7-(1,4-diazabicyclo[3.2.2]nonan-4-yl)-2-([{sup 18}F]fluorodibenzo[b,d]thiophene 5,5-dioxide), in nonhuman primates. The second aim was to assess further the quantification and test-retest variability of [{sup 18}F]ASEM in humans. PET scans with high specific activity [{sup 18}F]ASEM or [{sup 18}F]DBT-10 were acquired in three rhesus monkeys (one male, two female), and the kinetic properties of these radiotracers were compared. Additional [{sup 18}F]ASEM PET scans with blocking doses of nicotine, varenicline, and cold ASEM were acquired separately in two animals. Next, six human subjects (five male, one female) were imaged with [{sup 18}F]ASEM PET for 180 min, and arterial sampling was used to measure the parent input function. Different modeling approaches were compared to identify the optimal analysis method and scan duration for quantification of [{sup 18}F]ASEM distribution volume (V{sub T}). In addition, retest scans were acquired in four subjects (three male, one female), and the test-retest variability of V{sub T} was assessed. In the rhesus monkey brain [{sup 18}F]ASEM and [{sup 18}F]DBT-10 exhibited highly similar kinetic profiles. Dose-dependent blockade of [{sup 18}F]ASEM binding was observed, while administration of either nicotine or varenicline did not change [{sup 18}F]ASEM V{sub T}. [{sup 18}F]ASEM was selected for further validation because it has been used in humans. Accurate quantification of [{sup 18}F]ASEM V{sub T} in humans was achieved using multilinear analysis with at least 90 min of data acquisition, resulting in V{sub T} values ranging from 19.6 ± 2

Synthesis and characterization of new fluoride-containing manganese vanadates A{sub 2}Mn{sub 2}V{sub 2}O{sub 7}F{sub 2} (A=Rb, Cs) and Mn{sub 2}VO{sub 4}F

Energy Technology Data Exchange (ETDEWEB)

Sanjeewa, Liurukara D. [Department of Chemistry and Center for Optical Materials Science and Engineering Technologies (COMSET), Clemson University, Clemson, SC 29634-0973 (United States); McGuire, Michael A. [Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 (United States); Smith Pellizzeri, Tiffany M.; McMillen, Colin D. [Department of Chemistry and Center for Optical Materials Science and Engineering Technologies (COMSET), Clemson University, Clemson, SC 29634-0973 (United States); Ovidiu Garlea, V. [Quantum Condensed Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 (United States); Willett, Daniel; Chumanov, George [Department of Chemistry and Center for Optical Materials Science and Engineering Technologies (COMSET), Clemson University, Clemson, SC 29634-0973 (United States); Kolis, Joseph W., E-mail: kjoseph@clemson.edu [Department of Chemistry and Center for Optical Materials Science and Engineering Technologies (COMSET), Clemson University, Clemson, SC 29634-0973 (United States)

2016-09-15

Large single crystals of A{sub 2}Mn{sub 2}V{sub 2}O{sub 7}F{sub 2} (A=Rb, Cs) and Mn{sub 2}VO{sub 4}F were grown using a high-temperature (~600 °C) hydrothermal technique. Single crystal X-ray diffraction and powder X-ray diffraction were utilized to characterize the structures, which both possess MnO{sub 4}F{sub 2} building blocks. The A{sub 2}Mn{sub 2}V{sub 2}O{sub 7}F{sub 2} series crystallizes as a new structure type in space group Pbcn (No. 60), Z=4 (Rb{sub 2}Mn{sub 2}V{sub 2}O{sub 7}F{sub 2}: a=7.4389(17) Å, b=11.574(3) Å, c=10.914(2) Å; Cs{sub 2}Mn{sub 2}V{sub 2}O{sub 7}F{sub 2}: a=7.5615(15) Å, b=11.745(2) Å, c=11.127(2) Å). The structure is composed of zigzag chains of edge-sharing MnO{sub 4}F{sub 2} units running along the a-axis, and interconnected through V{sub 2}O{sub 7} pyrovanadate groups. Temperature dependent magnetic susceptibility measurements on this interesting one-dimensional structural feature based on Mn{sup 2+} indicated that Cs{sub 2}Mn{sub 2}V{sub 2}O{sub 7}F{sub 2} is antiferromagnetic with a Neél temperature, T{sub N}=~3 K and a Weiss constant, θ, of −11.7(1) K. Raman and infrared spectra were also analyzed to identify the fundamental V–O vibrational modes in Cs{sub 2}Mn{sub 2}V{sub 2}O{sub 7}F{sub 2}. Mn{sub 2}(VO{sub 4})F crystalizes in the monoclinic space group of C2/c (no. 15), Z=8 with unit cell parameters of a=13.559(2) Å, b=6.8036(7) Å, c=10.1408(13) Å and β=116.16(3)°. The structure is associated with those of triplite and wagnerite. Dynamic fluorine disorder gives rise to complex alternating chains of five-and six-coordinate Mn{sup 2+}. These interpenetrating chains are additionally connected through isolated VO{sub 4} tetrahedra to form the condensed structure. - Graphical abstract: New vanadate fluorides A{sub 2}Mn{sub 2}V{sub 2}O{sub 7}F{sub 2} (A=Rb, Cs) and Mn{sub 2}(VO{sub 4})F have been synthesized hydrothermally. Upon cooling, the one-dimensional Mn(II) substructure results in antiferromagnetic

Increased frequency of co-existing JAK2 exon-12 or MPL exon-10 mutations in patients with low JAK2(V617F) allelic burden.

Science.gov (United States)

Nussenzveig, Roberto H; Pham, Ha T; Perkins, Sherrie L; Prchal, Josef T; Agarwal, Archana M; Salama, Mohamed E

2016-01-01

The frequency of co-existing JAK2(V617F)/MPL and JAK2(V617F)/JAK2 exon-12 mutations has not been previously investigated in MPNs. Poor survival was reported in primary myelofibrosis with low JAK2(V617F) allelic burden. However, mutational status of JAK2 exon-12 or MPL were not reported in these patients. This study developed a cost-effective multiplex high resolution melt assay that screens for mutations in JAK2 gene exons-12 and -14 ((V617F)) and MPL gene exon-10. Co-existing mutations with JAK2(V617F) were detected in 2.9% (6/208; two JAK2 exon-12 and four MPL exon-10) patient specimens with known JAK2(V617F) (allelic-burden range: 0.1-96.8%). Co-existing mutations were detected in specimens with MPL exon-10 mutation should be pursued.

Melting curve analysis after T allele enrichment (MelcaTle as a highly sensitive and reliable method for detecting the JAK2V617F mutation.

Directory of Open Access Journals (Sweden)

Soji Morishita

Full Text Available Detection of the JAK2V617F mutation is essential for diagnosing patients with classical myeloproliferative neoplasms (MPNs. However, detection of the low-frequency JAK2V617F mutation is a challenging task due to the necessity of discriminating between true-positive and false-positive results. Here, we have developed a highly sensitive and accurate assay for the detection of JAK2V617F and named it melting curve analysis after T allele enrichment (MelcaTle. MelcaTle comprises three steps: 1 two cycles of JAK2V617F allele enrichment by PCR amplification followed by BsaXI digestion, 2 selective amplification of the JAK2V617F allele in the presence of a bridged nucleic acid (BNA probe, and 3 a melting curve assay using a BODIPY-FL-labeled oligonucleotide. Using this assay, we successfully detected nearly a single copy of the JAK2V617F allele, without false-positive signals, using 10 ng of genomic DNA standard. Furthermore, MelcaTle showed no positive signals in 90 assays screening healthy individuals for JAK2V617F. When applying MelcaTle to 27 patients who were initially classified as JAK2V617F-positive on the basis of allele-specific PCR analysis and were thus suspected as having MPNs, we found that two of the patients were actually JAK2V617F-negative. A more careful clinical data analysis revealed that these two patients had developed transient erythrocytosis of unknown etiology but not polycythemia vera, a subtype of MPNs. These findings indicate that the newly developed MelcaTle assay should markedly improve the diagnosis of JAK2V617F-positive MPNs.

Filtering reaction dynamics using nearside-farside theory and local angular momentum theory: application to the angular scattering of the H + D2(v(i) = 0, j(i) = 0) --> HD(v(f) = 3, j(f) = 0) + D reaction in the energy and time domains.

Science.gov (United States)

Monks, P D D; Connor, J N L; Bouakline, F

2009-04-23

We investigate methods for filtering reaction mechanisms in the angular scattering of the state-to-state reaction, H + D(2)(v(i) = 0, j(i) = 0, m(i) = 0) --> HD(v(f) = 3, j(f) = 0, m(f) = 0) + D, where v(i), j(i), and m(i) and v(f), j(f), and m(f) are initial and final vibrational, rotational, and helicity quantum numbers, respectively. The input to our filtrations is a new set of accurate quantum scattering matrix elements for total energies in the range 1.52-2.50 eV (in steps of 0.01 eV) and for total angular momentum quantum numbers in the range, 0-40, in steps of unity. We filter reaction mechanisms in both the energy domain and the time domain. The time-domain calculations employ the plane wave packet formulation of time-dependent scattering. The theoretical tools used are nearside-farside (NF) analysis of partial wave series for scattering amplitudes, together with NF local angular momentum (LAM) theory. An energy-domain LAM analysis reveals the existence of an important dynamical feature in the N scattering, a "trench" which bisects the (energy, angle) plane. We use the location of this trench to approximately filter two reaction mechanisms. Transformation to the time domain demonstrates that the two reaction mechanisms correspond to direct and delayed (by about 25 fs) scattering. Further analysis, including filtration in the time domain, shows that the pronounced LAM trench arises from the interference of the energy-domain analogues of the time-direct and time-delayed scattering. Our theory and results provide the first successful demonstration of reaction mechanism filtering carried out directly in the (energy, angle) domain. The calculations and results in this paper extend and complement earlier research reported by Monks, Connor, and Althorpe (Monks, P. D. D.; Connor, J. N. L.; Althorpe, S. C. J. Phys. Chem. A 2006, 110, 741; J. Phys. Chem. A 2007, 111, 10302).

The Cr Redox Record of fO2 Variation in Angrites. Evidence for Redox Conditions of Angrite Petrogenesis and Parent Body

Science.gov (United States)

Shearer, Charles K.; Bell, Aaron S.; Burger, Paul V.; Papike, James J.; Jones, John; Le, Loan

2016-01-01

Angrites represent some of the earliest stages of planetesimal differentiation. Not surprisingly, there is no simple petrogenetic model for their origin. Petrogenesis has been linked to both magmatic and impact processes. Studies demonstrated that melting of chondritic material (e.g. CM, CV) at redox conditions where pure iron metal is unstable (e.g., IW+1 to IW+2) produced angrite-like melts. Alternatively, angrites were produced at more reducing conditions (redox conditions during crystallization (e.g., Fe metal and a Fe-Ti oxide with potential Fe3+. There have been several estimates of fO2 for angrites. Most notably, experiments examined the variation of DEu/DGd with fO2, between plagioclase and fassaitic pyroxene in equilibrium with an angrite melt composition. They used their observations to estimate the fO2 of crystallization to be approximately IW+0.6 for angrite LEW 86010. This estimate is only a "snapshot" of fO2 conditions during co-crystallization of plagioclase and pyroxene. Preliminary XANES analyses of V redox state in pyroxenes from D'Orbigny reported changes in fO2 from IW-0.7 during early pyroxene crystallization to IW+0.5 during latter episodes of pyroxene crystallization [15]. As this was a preliminary report, it presented limited information concerning the effects of pyroxene orientation and composition on the V valence measurements, and the effect of melt composition on valence and partitioning behavior of V. A closer examination of fO2 as recorded by Cr valence state in olivine will allow us to test models for primordial melting of chondritic material to produce the angrite parent melts. Here, we report the our initial stages of examining the origin and conditions of primordial melting on the angrite parent body and test some of the above models by integrating an experimental study of Cr and V valence partitioning between olivine [OL] and an angrite melt, with micro-scale determinations of Cr and V oxidation state in OL in selected "volcanic

Super-Efficient Refrigerator Program (SERP) evaluation volume 2: Preliminary impact and market transformation assessment

Energy Technology Data Exchange (ETDEWEB)

Lee, A.D.; Conger, R.L.

1996-08-01

The Super Efficient Refrigerator Program (SERP) is a collaborative utility program intended to transform the market for energy-efficient and environmentally friendly refrigerators. It is one of the first examples of a large-scale {open_quotes}market transformation{close_quotes} energy efficiency program. This report documents the preliminary impact and market transformation evaluation of SERP ({open_quotes}the Program{close_quotes}). Pacific Northwest National Laboratory (PNNL) conducted this evaluation for the U.S. Department of Energy. This study focuses on the preliminary impact evaluation and market transformation assessment, but also presents limited process evaluation information. It is based on interviews with refrigerator dealers and manufacturers, interviews with utility participants, industry data, and information from the Program administrators. Results from this study complement those from prior process evaluation also conducted by PNNL. 42 refs., 5 figs., 4 tabs.

Measurement of K-electron capture probability in the decay of [sup 87]Y

Energy Technology Data Exchange (ETDEWEB)

Prasad, N.V.S.V.; Murty, G.S.K.; Rao, M.V.S.C.; Sastry, D.L. (Andhra Univ., Visakhapatnam (India). Labs. for Nuclear Research); Chintalapudi, S.N. (Inter University Consortium for DAE Facilities, Calcutta (India))

1993-04-01

The K-electron capture probability for the 1/2[sup -] to 3/2[sup -]transition in the decay of [sup 87]Y to the 873.0 keV level in the daughter [sup 87]Sr was measured for the first time using an x-[gamma] summing method. The experimental P[sub K] value was found to be 0.911 [+-] 0.047, in agreement with the theoretical value of 0.878. (author).

Preliminary assessment of LiF and alanine detectors for the dosimetry of proton therapy beams

International Nuclear Information System (INIS)

Fattibene, P.; Calicchia, A.; De Angelis, C.; Onori, S.; Egger, E.

1996-01-01

An experimental intercomparison between the proton response of LiF TLD-100 and alanine detectors is reported. The investigations were performed with LiF chips and alanine pellets in a 62 MeV proton beam at the Paul Scherrer Institut in Villigen (CH). Results were compared with reference dosimetry provided by Markus type parallel plate ionization chamber. The response of the detectors was studied, in a phantom, at different beam penetration depths in pristine and modulated beams. For both alanine and TL detectors, within the experimental uncertainty of the measurements, no significant energy dependence in the response was observed down to the Bragg peak region. The sensitivity of alanine and LiF detectors to protons was measured in the centre of modulated Bragg peak and no significant difference was found with respect to 60 Co. Contrary to LiF, alanine also offers a remarkable tissue equivalence which favours its choice for in-phantom dosimetry. (author)

Characterisation of a Personal TL Photon Dosemeter Based on Two LiF(Mg,Cu,P) Detectors in Terms of Hp(0.07,alpha) and Hp(10,alpha)

International Nuclear Information System (INIS)

Fantuzzi, E.; Monteventi, F.; Sermenghi, I.; Uleri, G.

1999-01-01

A study was undertaken to evaluate the response of the ENEA Photon Personal Dosemeter, slightly modified in design, in terms of H p (0.07, α) and H p (10, α). A filtration of 20 and 270 mg.cm -2 respectively for the two LiF(Mg,Cu,P) detectors employed was adopted according to a preliminary analytical simulation. The new dosemeter can measure both H p (0.07, α) with a maximum uncertainty of ±4% in the range from 10 to 200 keV and H p (10, α) with a maximum uncertainty of ±15% in the range from 10 keV to 1.25 MeV, using the detector responses together with a correction function with respect to their ratio. Moreover, tests were performed to evaluate the dosemeter angular response (0 deg., 20 deg., 40 deg., and 60 deg.) obtaining good results according to international recommendations. All irradiation tests were performed at SSL of ENEA Institute for Radiation Protection in Bologna using the ISO water phantom (30 x 30 x 15 cm 3 ) and standard ISO filtered X ray beams as well as 137 Cs and 60 Co sources. This work represents a preliminary type test of the ENEA photon personal dosemeter and allows its introduction in routine dosimetry practice in accordance with the ICRU reference quantities. (author)

Analyzing power in inclusive π+ and π- production at high xF with a 200 GeV polarized proton beam

International Nuclear Information System (INIS)

Adams, D.L.; Bonner, B.E.; Buchanan, J.A.; Clement, J.M.; Corcoran, M.D.; Cranshaw, J.; Nessi-Tedaldi, F.; Nessi, M.; Nguyen, C.; Phillips, G.C.; Roberts, J.B.; Skeens, J.; White, J.L.; Akchurin, N.; Langland, J.; Onel, Y.; Belikov, N.I.; Derevschikov, A.A.; Grachov, O.A.; Matulenko, Yu.A.; Meschanin, A.P.; Nurushev, S.B.; Patalakha, D.I.; Rykov, V.L.; Solovyanov, V.L.; Vasiliev, A.N.; Bystricky, J.; Lehar, F.; Lesquen, A. de; Cossairt, J.D.; Read, A.L.; En'yo, H.; Funahashi, H.; Goto, Y.; Imai, K.; Itow, Y.; Makino, S.; Masaike, A.; Miyake, K.; Nagamine, T.; Saito, N.; Yamashita, S.; Grosnick, D.P.; Hill, D.A.; Lopiano, D.; Ohashi, Y.; Spinka, H.; Stanek, R.W.; Underwood, D.G.; Yokosawa, A.; Iwatani, K.; Krueger, K.W.; Kuroda, K.; Michalowicz, A.; Luehring, F.C.; Miller, D.H.; Maki, T.; Pauletta, G.; Penzo, A.; Schiavon, P.; Zanetti, A.; Van Rossum, L.; Salvato, G.; Villari, A.; Takashima, R.; Takeutchi, F.; Tamura, N.; Tanaka, N.; Yoshida, T.

1991-01-01

The analyzing power in inclusive charged pion production has been measured using the 200 GeV Fermilab polarized proton beam. A striking dependence in χ F is observed in which A N increases from 0 to 0.42 with increasing χ F for the π + data and decreases from 0 to -0.38 with increasing χ F for π - data. The kinematic range covered is 0.2≤χ F ≤0.9 and 0.2≤p T ≤2.0 GeV/c. In a simple model our data indicate that at large χ F the transverse spin of the proton is correlated with that of its quark constituents. (orig.)

Preliminary studies with (/sup 18/F)haloperidol: a radioligand for in vivo studies of the dopamine receptors

Energy Technology Data Exchange (ETDEWEB)

Tewson, T J; Raichle, M E; Welch, M J [Washington Univ., St. Louis, MO (USA). Edward Mallinckrodt Inst. of Radiology

1980-06-16

The authors report a synthesis of (/sup 18/F)haloperidol of sufficiently high specific activity to permit the mapping of dopamine receptors in vivo in man using PET. The preliminary work with this radioligand in vivo in monkeys clearly suggests that haloperidol enters brain from blood by means of carrier-mediated, facilitated diffusion rather than simple diffusion. This rather surprising observation not only assumes special importance in the interpretation of in vivo pharmacokinetic data on dopamine receptors in man or animals but may also be important in considerations of the possible mode of action of this drug on the central nervous system.

Early evaluation of cerebral metabolic rate of glucose (CMRglu) with {sup 18}F-FDG PET/CT and clinical assessment in idiopathic normal pressure hydrocephalus (INPH) patients before and after ventricular shunt placement: preliminary experience

Energy Technology Data Exchange (ETDEWEB)

Calcagni, Maria Lucia; Lavalle, Mariadea; Leccisotti, Lucia; Giordano, Alessandro [Universita Cattolica del Sacro Cuore, Institute of Nuclear Medicine, Rome (Italy); Mangiola, Annunziato; De Bonis, Pasquale; Anile, Carmelo [Universita Cattolica del Sacro Cuore, Institute of Neurosurgery, Rome (Italy); Indovina, Luca [Universita Cattolica del Sacro Cuore, Institute of Physics, Rome (Italy); Marra, Camillo [Universita Cattolica del Sacro Cuore, Institute of Neurology, Rome (Italy); Pelliccioni, Armando [Istituto Nazionale per l' Assicurazione contro gli Infortuni sul Lavoro (INAIL), Rome (Italy)

2012-02-15

We evaluated the relationships between the cerebral metabolic rate of glucose (CMRglu) measured by dynamic {sup 18}F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) and the clinical and neuropsychological assessment before and after the surgical procedure in idiopathic normal pressure hydrocephalus (INPH) patients. Eleven selected INPH patients underwent clinical assessment (modified Rankin scale, Krauss scale, Larsson categorization system and Stein-Langfitt scale), cognitive evaluation (Mini-Mental State Examination, MMSE) and dynamic {sup 18}F-FDG PET/CT scan 3 days before and 1 week after ventricular shunt placement. After shunting, the global CMRglu significantly increased (2.95 {+-} 0.44 vs 4.38 {+-} 0.68, p = 10{sup -7}) in all INPH patients with a mean percentage value of 48.7%. After shunting, no significant change was found in the Evans ratio whereas a significant decrease in all clinical scale scores was observed. Only a slight reduction in the MMSE was found. After shunting, a significant correlation between the global CMRglu value and clinical assessment was found (R {sup 2} = 0.75, p = 0.024); indeed all clinical scale scores varied (decreasing) and the CMRglu value also varied (increasing) in all INPH patients. Our preliminary data show that changes in the CMRglu are promptly reversible after surgery and that there is a relationship between the early metabolic changes and clinical symptoms, independently from the simultaneous changes in the ventricular size. The remarkable and prompt improvement in the global CMRglu and in symptoms may also have important implications for the current concept of ''neuronal plasticity'' and for the cells' reactivity in order to recover their metabolic function. (orig.)

The onset of chromospheric activity among the A- and F- type stars

Science.gov (United States)

Simon, Theodore; Landsman, Wayne

1987-01-01

IUE observations of C II lambda1335 and C IV lambda1549 and ground-based observations of He I lambda5876 have previously discovered intense levels of chromospheric activity among early F type stars. Virtually all F dwarfs show stronger chromospheric and transition region emission than do the cooler and more deeply convective dwarf stars like the Sun. The IUE spectra and those of He lambda5876 place the onset of stellar activity along the main sequence near a color B - V = 0.28, which corresponds approximately to spectral type FO and an effective temperature of 7300 K. However, existing X-ray observations of A and F stars suggest that coronal activity may reach a peak blueward of this high temperature boundary at B - V = 0.28 before vanishing among the early and mid A-type stars. Discussed are preliminary results of a new effort to refine the location of the high temperature boundary to chromospheric activity among A- and F- type stars, making use of low dispersion short-wavelength spectra from the IUE archives from which the strengths of C IV, C II, and Lyman alpha emission have been measured.

Resolute Apt, Northwest Territories, Canada. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

Science.gov (United States)

1972-01-17

V6 10 T U779Z 68i, s4,2 0*0 9 43 9o,3 9295 91,e 9,1 7 ’,!) 92bo ci’O S700 U. (0. (’ I. # S) .6 4 r, ś 13,60 f, IU O 7 L 77*2 Bj.7 d 3 il 87s. 10.3...kI#? 67.7 67*7 60#3 bb,5 609S 6b.6 tbH,6 69.1 69.0 2 Boo ’Loh.: UFO b".0 00. 71l I I f1 74? 12 7. 1490 73#6 7306 73.6 73.Y 74o9 74,3 14,88 S700 (:Jet f

Evaluation of {sup 18}F-BCPP-EF for mitochondrial complex 1 imaging in the brain of conscious monkeys using PET

Energy Technology Data Exchange (ETDEWEB)

Tsukada, Hideo; Ohba, Hiroyuki; Kanazawa, Masakatsu; Kakiuchi, Takeharu; Harada, Norihiro [Hamamatsu Photonics K.K., Central Research Laboratory, Hamamatsu, Shizuoka (Japan)

2014-04-15

We have reported on the development of a novel PET probe, {sup 18}F-2-tert-butyl-4-chloro-5-{6-[2-(2-fluoroethoxy)-ethoxy] -pyridin-3-ylmethoxy}-2H-pyridazin-3-one ({sup 18}F-BCPP-EF), for quantitative imaging of mitochondrial complex 1 (MC-1) activity in the brain of the living rat. For clinical application in humans, translational research in the monkey was conducted. PET measurements with {sup 18}F-BCPP-EF were performed in young and old monkeys (Macaca mulatta) in a conscious state with arterial blood sampling. The binding specificity of {sup 18}F-BCPP-EF was evaluated with rotenone, a specific MC-1 inhibitor, in young animals. The binding (total distribution volume, V{sub T}) of {sup 18}F-BCPP-EF was calculated using Logan graphical analysis, and one-tissue compartment model (1-TC) and two-tissue compartment model (2-TC) analyses using a metabolite-corrected plasma input function. F-BCPP-EF was rapidly taken up into the brain just after intravenous injection, peaked between 10 and 20 min after injection, and was then gradually eliminated. The 2-TC analysis provided a better fit than the 1-TC analysis, and the V{sub T} values from the 2-TC analysis correlated well with those from the Logan plot. With predosing with rotenone, {sup 18}F-BCPP-EF showed a higher uptake peak in the brain, followed by more rapid elimination thereafter than in the vehicle condition, resulting in significant reductions in 2-TC V{sub T} values in all regions. In old animals, the kinetics of {sup 18}F-BCPP-EF were slightly slower with lower peak levels than in young animals, resulting age-related reductions in {sup 18}F-BCPP-EF binding in all brain regions. The present study demonstrated that {sup 18}F-BCPP-EF may be a potential PET probe for quantitative imaging MC-1 activity in the living brain using PET. (orig.)

Detection of the F meson production in the e+e--annihilation at a c.m. energy at 4.4 GeV

International Nuclear Information System (INIS)

Brandelik, R.

1981-01-01

The aim of this thesis had been to detect the F mesons via their decay into eta mesons. For this the eta rate between 4 and 5 GeV c.m. energy had been determined via the decay of the eta mesons into two photons. Energy and direction of the decay photons had been measured in the interior detector of DASP. As result of the analysis three c.m. energy intervals resulted with eta rates different from zero. An exceptionally evident eta signal of sigmasub(eta) = (5.15 +- 1.65)nb+-40% syst. error was measured for c.m. energies around 4.42 GeV and yielded thereby a first evidence for the F production. The detection of the F production resulted finally also in this energy interval from the measurement of the exclusive decay Fsup(+-)->πsup(+-)eta. From the additional detection of a correlation between produced eta mesons and low energy photons γsub(L) (Eγ + e - ->F + Fsup(-*) respectively e + e - ->Fsup(+*)Fsup(-*) was concluded. Thereby finally via a kinematic fit the detection of 6 events succeeded over a background of 0.2 events from e + e - ->F + Fsup(-*) respectively e + e - ->Fsup(+*)Fsup(-*) and Fsup(*)->γsub(L)F, Fsup(+-)->πsup(+-)eta,eta->2γ. Thereby the mass of F and Fsup(*) was determined to msub(F) = (2.04 +- 0.06)GeV and msub(F*) = (2.15 +- 0.06)MeV. The cross section for the 6 signal events was estimated to sigma(e + e - ->F + Fsup(-*))xB.R. (Fsup(+-)->πsup(+-)eta) = (0.41 +- 0.18)nb +-40% syst. error. (orig./HSI) [de

Validation of PROBA-V GEOV1 and MODIS C5 & C6 fAPAR Products in a Deciduous Beech Forest Site in Italy

Czech Academy of Sciences Publication Activity Database

Nestola, E.; Sanchez-Zapero, J.; Latorre, C.; Mazzenga, F.; Matteucci, G.; Calfapietra, Carlo; Camacho, F.

2017-01-01

Roč. 9, č. 2 (2017), č. článku 126. ISSN 2072-4292 Institutional support: RVO:86652079 Keywords : fAPAR * validation * PROBA-V GEOV1 * MODIS C5 * MODIS C6 * beech forest * up-scaling * GCOS requirements * in-situ comparison and evaluation Subject RIV: EH - Ecology, Behaviour OBOR OECD: Environmental sciences (social aspects to be 5.7) Impact factor: 3.244, year: 2016

Preliminary measurements of the 87Rb half-life by means of the needle counter

International Nuclear Information System (INIS)

Zastawny, A.; Rabsztyn, B.

1989-01-01

In order to test the detector and to obtain the experience before starting the exact measurements, the measurements of 87 Rb half-life have been made. RbNO 3 produced by Merck was used to prepare the samples. The samples were prepared by evaporation of RbNO 3 water solution on an aluminium foil of 2,9 mg/cm 2 . The water solutions were changed in the range from (3286,4±1,8) x 10 -6 to (480,85±0,3) x 10 -6 . The background of the aluminium foil was equal to (0,117±0,007) cpm. In other measurements the back-scattering effect of 87 Rb beta rays on the aluminium foil amounted to 4%, as the measurements were performed in the 2π geometry. The diameters of samples were about 10 mm. The specific radioactivities of samples were measured versus the surface changed in the range (60-320) μg/cm 2 . The extrapolated value of the half-life, corrected for the chemical purity of the compound (as declared by the manufacterer) is equal to (4,86±0,1) x 10 10 years. This value is in good agreement with commonly accepted value of 4,88 x 10 10 years and with another last measurements. 10 refs., 1 fig., 2 tabs. (author)

JAK2V617F Somatic Mutation In The General Population

DEFF Research Database (Denmark)

Nielsen, Camilla; Bojesen, Stig E; Nordestgaard, Børge G

2014-01-01

of myeloproliferative neoplasm from no disease (n=8 at re-examination) through essential thrombocythemia (n=20) and polycythemia vera (n=13) to primary myelofibrosis (n=7). Among those diagnosed with a myeloproliferative neoplasm only at re-examination in 2012, in the preceding years JAK2V617F mutation burden increased...

Comparisons of [{sup 18}F]-1-deoxy-1-fluoro-scyllo-inositol with [{sup 18}F]-FDG for PET imaging of inflammation, breast and brain cancer xenografts in athymic mice

Energy Technology Data Exchange (ETDEWEB)

McLarty, Kristin; Moran, Matthew D. [Department of Psychiatry, University of Toronto, Toronto, ON, M5T 1R8 (Canada); PET Centre, Centre for Addiction and Mental Health, Toronto, ON, M5T 1R8 (Canada); Scollard, Deborah A.; Chan, Conrad [Department of Pharmaceutical Sciences, University of Toronto, Toronto, ON, M5S 3M2 (Canada); Sabha, Nesrin; Mukherjee, Joydeep; Guha, Abhijit [Arthur and Sonia Labatt Brain Tumour Research Centre, Hospital for Sick Children, University of Toronto, ON, M5G 1X8 (Canada); McLaurin, JoAnne [Centre for Research in Neurodegenerative Diseases, University of Toronto, Toronto, ON, M5S 3H2 (Canada); Nitz, Mark [Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6 (Canada); Houle, Sylvain; Wilson, Alan A. [Department of Psychiatry, University of Toronto, Toronto, ON, M5T 1R8 (Canada); PET Centre, Centre for Addiction and Mental Health, Toronto, ON, M5T 1R8 (Canada); Reilly, Raymond M., E-mail: raymond.reilly@utoronto.ca [Department of Pharmaceutical Sciences, University of Toronto, Toronto, ON, M5S 3M2 (Canada); Toronto General Research Institute, University Health Network, Toronto, ON, M5G 2M9 (Canada); Department of Medical Imaging, University of Toronto, Toronto, ON, M5S 3M2 (Canada); Vasdev, Neil, E-mail: neil.vasdev@utoronto.ca [Department of Psychiatry, University of Toronto, Toronto, ON, M5T 1R8 (Canada); PET Centre, Centre for Addiction and Mental Health, Toronto, ON, M5T 1R8 (Canada)

2011-10-15

Introduction: The aim of the study was to evaluate the uptake of [{sup 18}F]-1-deoxy-1-fluoro-scyllo-inositol ([{sup 18}F]-scyllo-inositol) in human breast cancer (BC) and glioma xenografts, as well as in inflammatory tissue, in immunocompromised mice. Studies of [{sup 18}F]-2-fluoro-2-deoxy-D-glucose ([{sup 18}F]-FDG) under the same conditions were also performed. Methods: Radiosynthesis of [{sup 18}F]-scyllo-inositol was automated using a commercial synthesis module. Tumour, inflammation and normal tissue uptakes were evaluated by biodistribution studies and positron emission tomography (PET) imaging using [{sup 18}F]-scyllo-inositol and [{sup 18}F]-FDG in mice bearing subcutaneous MDA-MB-231, MCF-7 and MDA-MB-361 human BC xenografts, intracranial U-87 MG glioma xenografts and turpentine-induced inflammation. Results: The radiosynthesis of [{sup 18}F]-scyllo-inositol was automated with good radiochemical yields (24.6%{+-}3.3%, uncorrected for decay, 65{+-}2 min, n=5) and high specific activities ({>=}195 GBq/{mu}mol at end of synthesis). Uptake of [{sup 18}F]-scyllo-inositol was greatest in MDA-MB-231 BC tumours and was comparable to that of [{sup 18}F]-FDG (4.6{+-}0.5 vs. 5.5{+-}2.1 %ID/g, respectively; P=.40), but was marginally lower in MDA-MB-361 and MCF-7 xenografts. Uptake of [{sup 18}F]-scyllo-inositol in inflammation was lower than [{sup 18}F]-FDG. While uptake of [{sup 18}F]-scyllo-inositol in intracranial U-87 MG xenografts was significantly lower than [{sup 18}F]-FDG, the tumour-to-brain ratio was significantly higher (10.6{+-}2.5 vs. 2.1{+-}0.6; P=.001). Conclusions: Consistent with biodistribution studies, uptake of [{sup 18}F]-scyllo-inositol was successfully visualized by PET imaging in human BC and glioma xenografts, with lower accumulation in inflammatory tissue than [{sup 18}F]-FDG. The tumour-to-brain ratio of [{sup 18}F]-scyllo-inositol was also significantly higher than that of [{sup 18}F]-FDG for visualizing intracranial glioma xenografts in

A theoretical model of the M87 jet

International Nuclear Information System (INIS)

Falle, S.A.E.G.; Wilson, M.J.

1985-01-01

This paper describes a theoretical model of the knots in the M87 jet based on the idea that it is a steady fluid jet propagating through a non-uniform atmosphere. It is argued that knots D, E and F can be explained by the jet being underexpanded as it emerges from the central source, while knot A is due to reconfinement of the jet. Very high resolution numerical calculations are used to show that good agreement with the observed positions of the knots can be obtained with reasonable jet parameters and an atmosphere consistent with the X-ray observations. (author)

TL response of Eu activated LiF nanocubes irradiated by 85 MeV carbon ions

Energy Technology Data Exchange (ETDEWEB)

Salah, Numan, E-mail: nsalah@kau.edu.sa [Center of Nanotechnology, King Abdulaziz University, Jeddah 21589 (Saudi Arabia); Alharbi, Najlaa D. [Sciences Faculty for Girls, King Abdulaziz University, Jeddah 21589 (Saudi Arabia); Habib, Sami S. [Center of Nanotechnology, King Abdulaziz University, Jeddah 21589 (Saudi Arabia); Lochab, S.P. [Inter-University Accelerator Centre, Aruna Asaf Ali Marg, New Delhi 110067 (India)

2015-09-01

Carbon ions were found to be effective for cancer treatment. These heavy ions have a high relative biological effectiveness compared to those of photons. They have higher linear energy transfer and sharper Bragg peak with a very excellent local tumor control. However, the dose of these swift heavy ions needs to be measured with great accuracy. Lithium fluoride (LiF) is a highly sensitive phosphor widely used for radiation dosimetry. In this work Eu activated LiF nanocubes were exposed to 85 MeV C{sup 6+} ion beam and evaluated for their thermoluminescence (TL) response. Pellet forms of this nanomaterial were exposed to these ions in the fluence range 10{sup 9}–10{sup 13} ions/cm{sup 2}. The obtained result shows a prominent TL glow peak at around 320 °C, which is different than that induced by gamma rays. This glow peak exhibits a linear response in the range 10{sup 9}–10{sup 12} ions/cm{sup 2}, corresponding to the equivalent absorbed doses 0.273–273 kGy. The absorbed doses, penetration depths and main energy loss were calculated using TRIM code based on the Monte Carlo simulation. The supralinearity function and stopping power in this nanomaterial were also studied. The modification induced in the glow curve structure as a result of changing irradiation type might be utilized to use LiF:Eu nanocubes as a dosimeter for mixed filed radiations. Moreover, the wide linear response of LiF:Eu nanocubes along with the low fading are another imperative results suggesting that this nanomaterial might be a good candidate for carbon ions dosimetry.

TL response of Eu activated LiF nanocubes irradiated by 85 MeV carbon ions

International Nuclear Information System (INIS)

Salah, Numan; Alharbi, Najlaa D.; Habib, Sami S.; Lochab, S.P.

2015-01-01

Carbon ions were found to be effective for cancer treatment. These heavy ions have a high relative biological effectiveness compared to those of photons. They have higher linear energy transfer and sharper Bragg peak with a very excellent local tumor control. However, the dose of these swift heavy ions needs to be measured with great accuracy. Lithium fluoride (LiF) is a highly sensitive phosphor widely used for radiation dosimetry. In this work Eu activated LiF nanocubes were exposed to 85 MeV C 6+ ion beam and evaluated for their thermoluminescence (TL) response. Pellet forms of this nanomaterial were exposed to these ions in the fluence range 10 9 –10 13 ions/cm 2 . The obtained result shows a prominent TL glow peak at around 320 °C, which is different than that induced by gamma rays. This glow peak exhibits a linear response in the range 10 9 –10 12 ions/cm 2 , corresponding to the equivalent absorbed doses 0.273–273 kGy. The absorbed doses, penetration depths and main energy loss were calculated using TRIM code based on the Monte Carlo simulation. The supralinearity function and stopping power in this nanomaterial were also studied. The modification induced in the glow curve structure as a result of changing irradiation type might be utilized to use LiF:Eu nanocubes as a dosimeter for mixed filed radiations. Moreover, the wide linear response of LiF:Eu nanocubes along with the low fading are another imperative results suggesting that this nanomaterial might be a good candidate for carbon ions dosimetry

Characterization and Prognosis Significance of JAK2 (V617F), MPL, and CALR Mutations in Philadelphia-Negative Myeloproliferative Neoplasms

OpenAIRE

Singdong, Roongrudee; Siriboonpiputtana, Teerapong; Chareonsirisuthigul, Takol; Kongruang, Adcharee; Limsuwanachot, Nittaya; Sirirat, Tanasan; Chuncharunee, Suporn; Rerkamnuaychoke, Budsaba

2016-01-01

Background: The discovery of somatic acquired mutations of JAK2 (V617F) in Philadelphia-negative myeloproliferative neoplasms (Ph-negative MPNs) including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF) has not only improved rational disease classification and prognostication but also brings new understanding insight into the pathogenesis of diseases. Dosage effects of the JAK2 (V617F) allelic burden in Ph-negative MPNs may partially influence clinical ...

Three-dimensional recoil-ion momentum analyses in 8.7 MeV O7+-He collisions

International Nuclear Information System (INIS)

Kambara, T.; Tang, J.Z.; Awaya, Y.

1995-01-01

Using high-resolution recoil-ion momentum spectroscopy we have measured the differential cross sections of single-electron capture and target single-ionization processes for 8.7 MeV O 7+ -He collisions as functions of scattering angle. A transverse momentum resolution of ±0.2 au, which corresponds to an angular resolution of about ±1.5x10 -6 rad for the projectile scattering angle, was obtained by intersecting a well collimated O 7+ beam with a target of a supersonic He jet from a pre-cooled gas and by measuring the recoil-ion transverse momentum. For the single capture reaction, information on the n-value of the electron final state in O 6+ (1snl) is obtained from the longitudinal momentum of the recoil ions. In pure single-electron capture, the dominant contributions to capture were found to be those from the n=4 and higher states, whereas single capture accompanied by the ionization of the second target electron mainly populates n=2 to n=4 states. Furthermore, the measured transverse momentum distribution differs significantly between pure single capture and capture with simultaneous ionization. The measured data for the pure capture process compare favourably with theoretical results based on a molecular-state expansion method. Other experimental data are discussed in terms of the classical overbarrier model. (author)

Preliminary Seismic Performance Evaluation of RPS Cabinet in a Research Reactor

Energy Technology Data Exchange (ETDEWEB)

Kwag, Shinyoung; Oh, Jinho; Lee, Jongmin; Kim, Youngki [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

2013-05-15

This RPS cabinet mainly provides the operators with the physical interface to monitor and handle the RPS. The objective of this paper is to perform seismic analyses and evaluate the preliminary structural integrity and seismic capacity of the RPS cabinet. For this purpose, a 3-D finite element model of the RPS cabinet is developed and its modal analyses are carried out for analyzing the dynamic characteristics. Response time history analyses and related safety evaluation are performed for the RPS cabinet subjected to seismic loads. Finally, the seismic margin and seismic fragility of the RPS cabinet are investigated. The seismic analysis, and preliminary structural integrity and seismic margin of the RPS cabinet under self weight and seismic load have been evaluated. For this purpose, 3-D finite element models of the RPS cabinet were developed. A modal analysis, response time history analysis, and seismic fragility analysis were then performed. From the structural analysis results, the RPS cabinet is below the structural design limit under PGA 0.3g (hor.) and 0.2g (ver.) and structurally withstands until PGA 3g (hor.) and 2g (ver.)

Pionic decays of phi (1020) and f' (1514)

International Nuclear Information System (INIS)

Pasupathy, J.; Singh, C.A.

1978-01-01

The decay amplitudes phi (1020) → 3π and f' (1514) → ππ are calculated using unitarity corrections to the Okubo-Zweig-Iizuka rule. We use dispersion relations for the amplitudes phi → rhoπ and f' → ππ and evaluate the contributions arising from intermediate states such as KK-bar, K*K-bar, etc. The KK-bar intermediate state contributes to the absorptive part of the amplitude phi → rhoπ a value which corresponds to a partial width GAMMA (phi → 3π) of 150 keV. The contribution of the KK-bar and K*K-bar + K-barK* to the dispersive part of the amplitude phi → rhoπ within the context of approximations employed by us is somewhat larger and corresponds to a partial width of 900 keV for phi → 3π. For the f' → ππ amplitude we have evaluated the contributions of the KK-bar, etaeta, and K*K-bar + K-bar*K intermediate states using the dual model for the amplitudes KK-bar → ππ, etaeta → ππ, and K*K-bar → ππ. The effective strength of the amplitude f' → ππ arising from such unitarity corrections to the Okubo-Zweig-Iizuka rule corresponds to a partial width GAMMA (f' → ππ) of 170 keV via the absorptive part and a value of 500 keV via the dispersive part

Characterization of an apparently synonymous F5 mutation causing aberrant splicing and factor V deficiency.

Science.gov (United States)

Nuzzo, F; Bulato, C; Nielsen, B I; Lee, K; Wielders, S J; Simioni, P; Key, N S; Castoldi, E

2015-03-01

Coagulation factor V (FV) deficiency is a rare autosomal recessive bleeding disorder. We investigated a patient with severe FV deficiency (FV:C mutation in exon 4 (c.578G>C, p.Cys193Ser), predicting the abolition of a conserved disulphide bridge, and an apparently synonymous variant in exon 8 (c.1281C>G). The observation that half of the patient's F5 mRNA lacked the last 18 nucleotides of exon 8 prompted us to re-evaluate the c.1281C>G variant for its possible effects on splicing. Bioinformatics sequence analysis predicted that this transversion would activate a cryptic donor splice site and abolish an exonic splicing enhancer. Characterization in a F5 minigene model confirmed that the c.1281C>G variant was responsible for the patient's splicing defect, which could be partially corrected by a mutation-specific morpholino antisense oligonucleotide. The aberrantly spliced F5 mRNA, whose stability was similar to that of the normal mRNA, encoded a putative FV mutant lacking amino acids 427-432. Expression in COS-1 cells indicated that the mutant protein is poorly secreted and not functional. In conclusion, the c.1281C>G mutation, which was predicted to be translationally silent and hence neutral, causes FV deficiency by impairing pre-mRNA splicing. This finding underscores the importance of cDNA analysis for the correct assessment of exonic mutations. © 2014 John Wiley & Sons Ltd.

Partial sulfonation of PVdF-co-HFP: A preliminary study and characterization for application in direct methanol fuel cell

International Nuclear Information System (INIS)

Das, Suparna; Kumar, Piyush; Dutta, Kingshuk; Kundu, Patit Paban

2014-01-01

Highlights: • Synthesis of sulfonated PVdF-co-HFP by reacting with chlorosulfonic acid. • Maximum degree of sulfonation and best properties were obtained for 7 h reaction. • A maximum water uptake value of 20% was obtained. • A maximum IEC value of 0.42 meq g −1 was obtained. • A methanol permeability of 2.44 × 10 −7 cm 2 s −1 was obtained. - Abstract: Sulfonation of PVdF-co-HFP was conducted by treating the copolymer with chlorosulfonic acid. The efficiency of this sulfonated copolymer towards application as a polymer electrolyte membrane in direct methanol fuel cell (DMFC) was evaluated. For this purpose, we determined the thermal stability, water uptake, ion exchange capacity (IEC), methanol crossover, and proton conductivity of the prepared membranes as functions of duration and degree of sulfonation. The characteristic aromatic peaks obtained in the FT-IR spectra confirmed the successful sulfonation of PVdF-co-HFP. The effect of sulfonation on the semi-crystalline nature of pure PVdF-co-HFP was determined from XRD analysis. Water uptake results indicated that a sulfonation time of 7 h produced maximum water uptake value of about 20%, with a corresponding IEC and proton conductivity values of about 0.42 meq g −1 and 0.00375 S cm −1 respectively. The maximum current density was recorded to be 30 mA cm −2 at 0.2 V potential

Quality assurance program description: Hanford Waste Vitrification Plant, Part 1

International Nuclear Information System (INIS)

1992-01-01

This document describes the Department of Energy's Richland Field Office (DOE-RL) quality assurance (QA) program for the processing of high-level waste as well as the Vitrification Project Quality Assurance Program for the design and construction of the Hanford Waste Vitrification Plant (HWVP). It also identifies and describes the planned activities that constitute the required quality assurance program for the HWVP. This program applies to the broad scope of quality-affecting activities associated with the overall HWVP Facility. Quality-affecting activities include designing, purchasing, fabricating, handling, shipping, storing, cleaning, erecting, installing, inspecting, testing, maintaining, repairing, and modifying. Also included are the development, qualification, and production of waste forms which may be safely used to dispose of high-level radioactive waste resulting from national defense activities. The HWVP QA program is made up of many constituent programs that are being implemented by the participating organizations. This Quality Assurance program description is intended to outline and define the scope and application of the major programs that make up the HWVP QA program. It provides a means by which the overall program can be managed and directed to achieve its objectives. Subsequent parts of this description will identify the program's objectives, its scope, application, and structure

Complementary roles of tumour specific PET tracer {sup 18}F-FAMT to {sup 18}F-FDG PET/CT for the assessment of bone metastasis

Energy Technology Data Exchange (ETDEWEB)

Morita, Motoho [Gunma University Hospital, Department of General Medicine, Maebashi, Gunma (Japan); Gunma University Graduate School of Medicine, Department of Diagnostic Radiology and Nuclear Medicine, Maebashi, Gunma (Japan); Higuchi, Tetsuya; Tokue, Azusa; Arisaka, Yukiko; Tsushima, Yoshito [Gunma University Graduate School of Medicine, Department of Diagnostic Radiology and Nuclear Medicine, Maebashi, Gunma (Japan); Achmad, Arifudin [Gunma University Graduate School of Medicine, Department of Diagnostic Radiology and Nuclear Medicine, Maebashi, Gunma (Japan); Gadjah Mada University, Department of Radiology, Faculty of Medicine, Yogyakarta (Indonesia)

2013-10-15

The usefulness of {sup 18}F-FDG PET/CT for bone metastasis evaluation has already been established. The amino acid PET tracer [{sup 18}F]-3-fluoro-alpha-methyl tyrosine ({sup 18}F-FAMT) has been reported to be highly specific for malignancy. We evaluated the additional value of {sup 18}F-FAMT PET/CT to complement {sup 18}F-FDG PET/CT in the evaluation of bone metastasis. This retrospective study included 21 patients with bone metastases of various cancers who had undergone both {sup 18}F-FDG and {sup 18}F-FAMT PET/CT within 1 month of each other. {sup 18}F-FDG-avid bone lesions suspicious for malignancy were carefully selected based on the cut-off value for malignancy, and the SUVmax of the {sup 18}F-FAMT in the corresponding lesions were evaluated. A total of 72 {sup 18}F-FDG-positive bone lesions suspected to be metastases in the 21 patients were used as the reference standard. {sup 18}F-FAMT uptake was found in 87.5 % of the lesions. In the lesions of lung cancer origin, the uptake of the two tracers showed a good correlation (40 lesions, r = 0.68, P < 0.01). Bone metastatic lesions of oesophageal cancer showed the highest average of {sup 18}F-FAMT uptake. Bone metastatic lesions of squamous cell carcinoma showed higher {sup 18}F-FAMT uptake than those of adenocarcinoma. No significant difference in {sup 18}F-FAMT uptake was seen between osteoblastic and osteolytic bone metastatic lesions. The usefulness of {sup 18}F-FAMT PET/CT for bone metastasis detection regardless of the lesion phenotype was demonstrated. The fact that {sup 18}F-FAMT uptake was confirmed by {sup 18}F-FDG uptake suggests that {sup 18}F-FAMT PET/CT has the potential to complement {sup 18}F-FDG PET/CT for the detection of bone metastases. (orig.)

The Ongoing Growth of the M87 Halo through Accretion Events

Directory of Open Access Journals (Sweden)

Alessia Longobardi

2015-12-01

Full Text Available Planetary nebulas (PNs offer a unique tool to investigate the outer regions of massive galaxies because their strong [OIII]λ5007Å emission line makes them detectable out to several effective radii from the galaxy’s centre. We use a deep and extended spectroscopic survey of PNs (∼300 objects to study the spatial distribution, the kinematics and the stellar populations in the extended outer halo of the bright elliptical galaxy M87 (NGC 4486 in the Virgo cluster. We show that in the Virgo core, M87 stellar halo and the intracluster light are two distinct dynamical components, with different velocity distributions. Moreover the synergy of the PN kinematical information and the deep V/B-band photometry revealed an ongoing accretion event in the outer regions of M87. This satellite accretion represents a non-negligible perturbation of the halo properties: beyond 60 kpc the M87 halo is still growing with 60% of its light being added by the accretion event at the distance where it is detected.

Fission-fragment angular distributions and total kinetic energies for 235U(n,f) from .18 to 8.83 MeV

International Nuclear Information System (INIS)

Meadows, J.W.; Budtz-Joergensen, C.

1982-01-01

A gridded ion chamber was used to measure the fission fragment angular distribution and total kinetic energy for the 235 U(n,f) reaction from 0.18 to 8.81 MeV neutron energy. The anisotropies are in generally good agreement with earlier measurements. The average total kinetic energy is approx. 0.2 MeV greater than the thermal value at neutron energies < 2 MeV and shows a sudden decrease of approx. 0.8 MeV between 4 and 5 MeV neutron energy, well below the (n, n'f) threshold. Possible causes of this decrease are a change in the mass distribution or decreased shell effects in the heavy fragment

Evaluation of caesium atomic fountain NICT-CsF1

International Nuclear Information System (INIS)

Kumagai, M.; Ito, H.; Kajita, M.; Hosokawa, M.

2008-01-01

In this paper, we describe the first caesium atomic fountain primary frequency standard NICT-CsF1 of National Institute of Information Communications Technology (NICT) in Tokyo, Japan. The structure of the NICT-CsF1 system and evaluation procedure of the systematic frequency shifts and their uncertainties are presented. Typically, NICT-CsF1 has a frequency stability of 4 * 10 -13 /τ 1/2 and a frequency uncertainty of 1.9 * 10 -15 . (authors)

FY87 Evaluation of Shipboard Electro-Optical Apparatus for Use in Law Enforcement and Search.

Science.gov (United States)

1987-12-01

track, toward B-5 Cuban waters. M/V Esjoci’s master requested course--may be having compass problems. Coast Guard Officer of the Deck told him to steer...Inspeced. NCICS: MACHINERYC111 0 N jiSu[MIhE DRL$ AN TAI Olw f nS it SDg ~. (’ATGUR O WAHROBEVTONAOOERTOA SUEkARsi ySE T L tl Ten,_ _ r. 1 , D( .I _j U ,_6...bridge six feet from our magnetic compass . The FLIR’s proximity to the compass caused approximately eight degrees deviation when energized. It also

pt and xF dependence of the polarization of Σ+ hyperons produced by 800 GeV/c protons

International Nuclear Information System (INIS)

Morelos, A.; Albuquerque, I.F.; Bondar, N.F.; Carrigan, R. Jr.; Chen, D.; Cooper, P.S.; Lisheng, D.; Denisov, A.S.; Dobrovolsky, A.V.; Dubbs, T.; Endler, A.M.F.; Escobar, C.O.; Foucher, M.; Golovtsov, V.L.; Gottschalk, H.; Gouffon, P.; Grachev, V.T.; Khanzadeev, A.V.; Kubantsev, M.A.; Kuropatkin, N.P.; Lach, J.; Lang Pengfei; Lebedenko, V.N.; Li Chengze; Li Yunshan; Luksys, M.; Mahon, J.R.P.; McCliment, E.; Newsom, C.; Pommot Maia, M.C.; Samsonov, V.M.; Schegelsky, V.A.; Shi Huanzhang; Smith, V.J.; Fukun, T.; Terentyev, N.K.; Timm, S.; Tkatch, I.I.; Uvarov, L.N.; Vorobyov, A.A.; Yan Jie; Wenheng, Z.; Zheng Shuchen; Zhong Yuanyuan

1995-01-01

We utilize the angle and momentum resolution of our apparatus to study the polarization of 375 GeV/c Σ + hyperons produced by 800 GeV/c protons incident on a Cu target. By examining in detail two of our high statistics data samples, we find evidence for structure in the p t dependence of Σ + polarization and are able to extract the x F dependence of the Σ + polarization and compare it with x F behavior in the Λ 0 and Ξ - systems

The JAK2 V617F allele burden in essential thrombocythemia, polycythemia vera and primary myelofibrosis

DEFF Research Database (Denmark)

Larsen, Thomas Stauffer; Pallisgaard, Niels; Møller, Michael Boe

2007-01-01

BACKGROUND AND OBJECTIVES: The JAK2 V617F tyrosine kinase mutation is present in the great majority of patients with polycythemia vera (PV), and approximately half of the patients with essential thrombocythemia (ET) and primary myelofibrosis (PMF). The three distinct disease entities may be consi......BACKGROUND AND OBJECTIVES: The JAK2 V617F tyrosine kinase mutation is present in the great majority of patients with polycythemia vera (PV), and approximately half of the patients with essential thrombocythemia (ET) and primary myelofibrosis (PMF). The three distinct disease entities may...... = 0.03), CD34 counts (P = 0.03), lactate dehydrogenase and Polycythemia Rubra Vera gene 1 levels (P = 0.03 and P

Nuclear data evaluation for 239Pu in the energy region 100 075. ev - 15 MeV

International Nuclear Information System (INIS)

Antsipov, G.V.; Bakhanovich, L.A.; Zharkov, V.F.

1981-06-01

The present work incorporates the results of the new nuclear data evaluation for 239 Pu. The complete file compiled by the authors in 1974 was practically completely re-examined. The most substantial changes were made above 0.5 keV. In the region of unresolved resonances (0.3-100 keV), the channel contribution to the process widths for a given state and some other aspects were taken into account. The fission cross section and the α-value were evaluated with regard for the correlation of experimental errors. The non-spherical potential with optimized parameters was used to make calculations by the optical and statistical models. The contribution of pre-equilibrium emission was taken into account in calculations of secondary neutron spectra. The new standard anti nusub(sp)( 252 Csub(f)) was used to calculate anti nusub(p). The 26-group constants and g-Westcott factors were obtained from the evaluated data. The complete file of the evaluated data for 239 Pu is presented in the ENDF/B format and conveyed to the Nuclear Data Section of the IAEA. (author)

Synthesis and crystal structure of triammine pentafluorido tantalum(V) [TaF{sub 5}(NH{sub 3}){sub 3}

Energy Technology Data Exchange (ETDEWEB)

Baer, Sebastian A.; Kraus, Florian [Department Chemie, Technische Universitaet Muenchen, Lichtenbergstrasse 4, 85747 Garching (Germany); Lozinsek, Matic [Department of Inorganic Chemistry and Technology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana (Slovenia)

2013-07-01

[Sr(HF){sub 3}(TaF{sub 6}){sub 2}] reacts with liquid ammonia under the formation of colorless crystals of triammine pentafluorido tantalum(V) [TaF{sub 5}(NH{sub 3}){sub 3}] (1). The structure was elucidated by low-temperature X-ray structure analysis. Compound 1 crystallizes in the monoclinic space group P2{sub 1}/c with a = 5.1525(6), b = 11.736(1), c = 10.171(1) Aa, β = 94.843(9) , V = 612.8(1) Aa{sup 3} at 123 K with Z = 4. Its structure displays discrete TaF{sub 5}(NH{sub 3}){sub 3} molecules, which are interconnected by N-H..F hydrogen bonds to form a complex three-dimensional network. The title compound is a rare example of a neutral, molecular, eight-coordinate tantalum species. (Copyright copyright 2013 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

Hardening and formation of dislocation structures in LiF crystals irradiated with MeV-GeV ions

CERN Document Server

Manika, I; Schwartz, K; Trautmann, C

2002-01-01

Material modifications of LiF crystals irradiated with Au, Pb and Bi ions of MeV to GeV energy are studied by means of microindentation measurements and dislocation etching. Above a critical irradiation fluence of 10 sup 9 ions/cm sup 2 , the microhardness can improve by a factor of 2 in the bulk and by more than 3 on the surface. Radiation-induced hardening follows the evolution of the energy loss along the ion path. Annealing experiments indicate that complex defect aggregates created in the tracks play a major role for the hardness change. Evidence for severe structural modifications is found when etching indentation impressions in highly irradiated crystals leading to similar pattern as in amorphous or micro-grained materials. Dislocation etching also reveals long-range stress fields extending far beyond the implantation zone deep into the nonirradiated crystal.

National uranium resource evaluation, preliminary report

International Nuclear Information System (INIS)

1976-06-01

The results of the initial phase of the National Uranium Resource Evaluation (NURE) are reported. NURE is a comprehensive nationwide program to evaluate uranium resources and to identify areas favorable for uranium exploration. Part I presents estimates of uranium ore reserves and potential resources available at costs (not prices) of $10, $15, and $30 per pound U 3 O 8 (uranium oxide). These estimates comprise the national uranium resource position. They are, however, preliminary because limitations of time and available geologic data prevented adequate assessment of some areas that may be favorable for potential resources. Part II presents the potential uranium resources for each of 13 regions, whose boundaries have been drawn chiefly on geologic considerations. The general geology is summarized, and the types of uranium deposits are described. Although limited geologic reconnaissance was done in various parts of the country, the report is based primarily on the compilation and evaluation of data in ERDA files. Mining companies furnished a substantial amount of information on exploration results, development, production, and future plans. Published, manuscript, and open-file reports by government agencies, universities, and research organizations were reviewed. In addition, many individuals affiliated with universities and with state and federal agencies provided supplemental geologic information. This was particularly helpful in the eastern and central states and in Alaska, where information on uranium occurrences is limited

NMR of 19F emulsions: methodological developments and application to evaluation of oxi-metry and dynamic biodistribution in the liver and spleen and to detection of tumor angiogenesis in the rodent brain

International Nuclear Information System (INIS)

Giraudeau, C.

2012-01-01

This study aimed at developing a method for detection of brain tumors at 7 tesla thanks to 19 F MRI contrast agents. We particularly assessed the potential of this method to highlight tumor angiogenesis with RGD-functionalized contrast agents targeting αvβ3integrin, a bio-marker over-expressed at the surface of new capillary blood vessels. Owing to low local concentrations in contrast agent, the first step consisted in optimizing a multi spin echo sequence dedicated to a well-known biocompatible per-fluorocarbon, perfluoro-octylbromide (PFOB). We show that careful adjustment of sequence parameters allows cancellation of J-modulation and T2 enhancement, and yields an excellent sensitivity in vitro. Our sequence was then tested for oxygenation measurements in the mouse liver and spleen after injection of a PFOB emulsion. The results demonstrate very good accuracy of the measurements after one single infusion of emulsion. We also perform a dynamic biodistribution study in order to monitor emulsion nano-particle uptake in the liver and spleen. Moreover, we show that stealth of emulsions grafted with different quantities of polyethylene glycol (PEG) can be assessed by fitting experimental data with a pharmacokinetic empirical model. Our sequence was finally used to visualize αvβ3-targeted nano-particles in a U87 glioblastoma mouse model. Concentrations found in tumors after injection of an RGD-functionalized emulsion and a control emulsion are compared. Concentrations are found to be significantly higher with the RGD emulsion than with the control emulsion, suggesting specific binding of functionalized nano-particles with αvβ3 integrin. The last part is dedicated to a new diffusion-weighted 19 F NMR spectroscopy sequence. This method aims at suppressing vascular signal coming from circulating PFOB nano-particles in order to evaluate signal coming from bound nano-particles only. (author) [fr

Impacts of P-f & Q-V Droop Control on MicroGrids Transient Stability

Science.gov (United States)

Zhao-xia, Xiao; Hong-wei, Fang

Impacts of P-f & Q-V droop control on MicroGrid transient stability was investigated with a wind unit of asynchronous generator in the MicroGrid. The system frequency stability was explored when the motor load starts and its load power changes, and faults of different types and different locations occurs. The simulations were done by PSCAD/EMTDC.

JAK2 and MPL gene mutations in V617F-negative myeloproliferative neoplasms.

NARCIS (Netherlands)

Siemiatkowska, A.M.; Bieniaszewska, M.; Hellmann, A.; Limon, J.

2010-01-01

We report three novel mutations in JAK2 exons 12, 19 and 25 in V617F-negative patients with polycythemia vera, essential thrombocythemia and idiopathic myelofibrosis. Scanning of JAK2 exons 12-25 and MPL exon 10 revealed the presence of JAK2 alterations in six and MPL W515L/K mutations in five of 34

The JAK2V617F and CALR exon 9 mutations are shared immunogenic neoantigens in hematological malignancy

DEFF Research Database (Denmark)

Holmstrom, Morten Orebo; Hasselbalch, Hans Carl; Andersen, Mads Hald

2017-01-01

Approximately 90% of patients with the hematological malignancies termed the chronic myeloproliferative neoplasms harbor either the JAK2V617F-mutation or CALR exon 9 mutation. Both of these are recognized by T-cells, which make the mutations ideal targets for cancer immune therapy as they are sha......Approximately 90% of patients with the hematological malignancies termed the chronic myeloproliferative neoplasms harbor either the JAK2V617F-mutation or CALR exon 9 mutation. Both of these are recognized by T-cells, which make the mutations ideal targets for cancer immune therapy...

SurF: an innovative framework in biosecurity and animal health surveillance evaluation.

Science.gov (United States)

Muellner, Petra; Watts, Jonathan; Bingham, Paul; Bullians, Mark; Gould, Brendan; Pande, Anjali; Riding, Tim; Stevens, Paul; Vink, Daan; Stärk, Katharina Dc

2018-05-16

Surveillance for biosecurity hazards is being conducted by the New Zealand Competent Authority, the Ministry for Primary Industries (MPI) to support New Zealand's biosecurity system. Surveillance evaluation should be an integral part of the surveillance life cycle, as it provides a means to identify and correct problems and to sustain and enhance the existing strengths of a surveillance system. The surveillance evaluation Framework (SurF) presented here was developed to provide a generic framework within which the MPI biosecurity surveillance portfolio, and all of its components, can be consistently assessed. SurF is an innovative, cross-sectoral effort that aims to provide a common umbrella for surveillance evaluation in the animal, plant, environment and aquatic sectors. It supports the conduct of the following four distinct components of an evaluation project: (i) motivation for the evaluation, (ii) scope of the evaluation, (iii) evaluation design and implementation and (iv) reporting and communication of evaluation outputs. Case studies, prepared by MPI subject matter experts, are included in the framework to guide users in their assessment. Three case studies were used in the development of SurF in order to assure practical utility and to confirm usability of SurF across all included sectors. It is anticipated that the structured approach and information provided by SurF will not only be of benefit to MPI but also to other New Zealand stakeholders. Although SurF was developed for internal use by MPI, it could be applied to any surveillance system in New Zealand or elsewhere. © 2018 2018 The Authors. Transboundary and Emerging Diseases Published by Blackwell Verlag GmbH.

Evaluation of gas-phase technetium decontamination and safety related experiments during FY 1994. A report of work in progress

Energy Technology Data Exchange (ETDEWEB)

Simmons, D.W.; Munday, E.B.

1995-05-01

Laboratory activities for FY94 included: evaluation of decontamination of Tc by gas-phase techniques, evaluation of diluted ClF{sub 3} for removing U deposits, evaluation of potential hazard of wet air inlekage into a vessel containing ClF{sub 3}, planning and preparation for experiments to assess hazard of rapid reaction of ClF{sub 3} and hydrated UO{sub 2}F{sub 2} or powdered Al, and preliminary evaluation of compatibility of Tenic valve seat material.

76 FR 28019 - Gregory R. Swecker, Beverly F. Swecker v. Midland Power Cooperative, State of Iowa; Notice of...

Science.gov (United States)

2011-05-13

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL11-39-000] Gregory R. Swecker, Beverly F. Swecker v. Midland Power Cooperative, State of Iowa; Notice of Complaint Take notice... 1978 (PURPA),\\1\\ Gregory R. Swecker and Beverly F. Swecker (Complainants) filed a petition requesting...

Radioactive waste shredding: Preliminary evaluation

International Nuclear Information System (INIS)

Soelberg, N.R.; Reimann, G.A.

1994-07-01

The critical constraints for sizing solid radioactive and mixed wastes for subsequent thermal treatment were identified via a literature review and a survey of shredding equipment vendors. The types and amounts of DOE radioactive wastes that will require treatment to reduce the waste volume, destroy hazardous organics, or immobilize radionuclides and/or hazardous metals were considered. The preliminary steps of waste receipt, inspection, and separation were included because many potential waste treatment technologies have limits on feedstream chemical content, physical composition, and particle size. Most treatment processes and shredding operations require at least some degree of feed material characterization. Preliminary cost estimates show that pretreatment costs per unit of waste can be high and can vary significantly, depending on the processing rate and desired output particle size

Concurrent MPL515 and JAK2V617F mutations in myelofibrosis: chronology of clonal emergence and changes in mutant allele burden over time.

Science.gov (United States)

Lasho, Terra L; Pardanani, Animesh; McClure, Rebecca F; Mesa, Ruben A; Levine, Ross L; Gilliland, D Gary; Tefferi, Ayalew

2006-12-01

MPLW515L/K and JAK2V617F can co-exist in myelofibrosis with myeloid metaplasia (MMM). The chronology of clonal emergence was studied in three such cases using serially stored bone marrow. At diagnosis, a major MPL515 mutant clone was accompanied by a minor JAK2V617F clone in all three instances. At 25 time points over a period of 4-8 years, allele burden fluctuated but remained high for MPLW515L/K and low for JAK2V617F. We conclude that MPLW515L/K and JAK2V617F are both early events in MMM and allele burden, rather than the mere presence of these mutations, might be relevant to phenotypic variation in myeloproliferative disorders.

Phenotypic evaluation of the resistance in F1 carnation populations to vascular wilt caused by Fusarium oxysporum f.sp. dianthi

Directory of Open Access Journals (Sweden)

Johana Carolina Soto-Sedano

2012-08-01

Full Text Available One of the most important phytosanitary problems of the carnation crops in Colombia and in the entire world is vascular wilting produced by Fusarium oxysporum f.sp. dianthi. Currently, an effective treatment against the pathogen does not exist; the search for resistant varieties has been the most successful method for control of this disease. Breeding programs are vital to solving the problem of the carnation fusariosis. The objective of this research was the phenotypic evaluation of carnation F1 populations, products of contrasting crossing, resistant per susceptible to F. oxysporum f.sp. dianthi, in order to determine if the resistance is inherited in the lines. This information will contribute to the selection of material and to the successful introduction of the resistant characteristic, whose expression is commercially acceptable, to the gene pool. The methodology adopted was a phenotypic evaluation of the response to the parasite in the population (450 individuals and in the parental. This evaluation estimated the area under the curve (AU DPC, using a scale of symptoms reported for carnation vascular wilt. Three different phenotypes were established with this evaluation. The moderately susceptible one is the predominant phenotype and an analysis of phenotypic frequencies was carried out on it. The results show that the individuals of the evaluated F1 population were distributed between two extreme ranges, resistant and susceptible; this shows that there is segregation for the trait resistant to F. oxysporum f.sp dianthi. We did not observe clearly differentiated classes, i.e. with complete absence or presence of the disease, indicating a possible control of the resistance in the evaluated carnation material, governed by more than one gene and with a possible additive genetic action

Development and preliminary analyses of material balance evaluation model in nuclear fuel cycle

International Nuclear Information System (INIS)

Matsumura, Tetsuo

1994-01-01

Material balance evaluation model in nuclear fuel cycle has been developed using ORIGEN-2 code as basic engine. This model has feature of: It can treat more than 1000 nuclides including minor actinides and fission products. It has flexibility of modeling and graph output using a engineering work station. I made preliminary calculation of LWR fuel high burnup effect (reloading fuel average burnup of 60 GWd/t) on nuclear fuel cycle. The preliminary calculation shows LWR fuel high burnup has much effect on Japanese Pu balance problem. (author)

(11)C-labeling and preliminary evaluation of vortioxetine as a PET radioligand

DEFF Research Database (Denmark)

Andersen, Valdemar L; Hansen, Hanne D; Herth, Matthias M

2014-01-01

. Preliminary evaluation of [(11)C]vortioxetine in a Danish Landrace pig showed rapid brain uptake and brain distribution in accordance with the pharmacological profile, all though an unexpected high binding in cerebellum was also observed. [(11)C]vortioxetine displayed slow tracer kinetics with peak uptake...

Identification and quantification of N alpha-acetylated Y. pestis fusion protein F1-V expressed in Escherichia coli using LCMS E.

Science.gov (United States)

Bariola, Pauline A; Russell, Brett A; Monahan, Steven J; Stroop, Steven D

2007-05-31

N-terminal acetylation in E coli is a rare event catalyzed by three known N-acetyl-transferases (NATs), each having a specific ribosomal protein substrate. Multiple, gram-scale lots of recombinant F1-V, a fusion protein constructed from Y. Pestis antigens, were expressed and purified from a single stably transformed E. coli cell bank. A variant form of F1-V with mass increased by 42-43 Da was detected in all purified lots by electrospray orthogonal acceleration time-of-flight mass spectrometry (MS). Peptide mapping LCMS localized the increased mass to an N-terminal Lys-C peptide, residues 1-24, and defined it as +42.0308+/-0.0231 Da using a LockSpray exact mass feature and a leucine enkaphalin mass standard. Sequencing of the variant 1-24 peptide by LCMS and high-energy collision induced dissociation (LCMS(E)) further localized the modification to the amino terminal tri-peptide ADL and identified the modification as N(alpha)-acetylation. The average content of N(alpha)-acetylated F1-V in five lots was 24.7+/-2.6% indicating that a stable acetylation activity for F1-V was established in the E. coli expression system. Alignment of the F1-V N-terminal sequence with those of other known N(alpha)-acetylated ectopic proteins expressed in E. coli reveals a substrate motif analogous to the eukaryote NatA' acetylation pathway and distinct from endogenous E. coli NAT substrates.

Hanford Waste Vitrification Plant technical background document for best available radionuclide control technology demonstration

Energy Technology Data Exchange (ETDEWEB)

Carpenter, A.B.; Skone, S.S.; Rodenhizer, D.G.; Marusich, M.V. (Ebasco Services, Inc., Bellevue, WA (USA))

1990-10-01

This report provides the background documentation to support applications for approval to construct and operate new radionuclide emission sources at the Hanford Waste Vitrification Plant (HWVP) near Richland, Washington. The HWVP is required to obtain permits under federal and state statutes for atmospheric discharges of radionuclides. Since these permits must be issued prior to construction of the facility, draft permit applications are being prepared, as well as documentation to support these permits. This report addresses the applicable requirements and demonstrates that the preferred design meets energy, environmental, and economic criteria for Best Available Radionuclide Control Technology (BARCT) at HWVP. 22 refs., 11 figs., 25 tabs.

Hanford Waste Vitrification Plant technical background document for best available radionuclide control technology demonstration

International Nuclear Information System (INIS)

Carpenter, A.B.; Skone, S.S.; Rodenhizer, D.G.; Marusich, M.V.

1990-10-01

This report provides the background documentation to support applications for approval to construct and operate new radionuclide emission sources at the Hanford Waste Vitrification Plant (HWVP) near Richland, Washington. The HWVP is required to obtain permits under federal and state statutes for atmospheric discharges of radionuclides. Since these permits must be issued prior to construction of the facility, draft permit applications are being prepared, as well as documentation to support these permits. This report addresses the applicable requirements and demonstrates that the preferred design meets energy, environmental, and economic criteria for Best Available Radionuclide Control Technology (BARCT) at HWVP. 22 refs., 11 figs., 25 tabs

40 CFR 763.87 - Analysis.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Analysis. 763.87 Section 763.87... Asbestos-Containing Materials in Schools § 763.87 Analysis. (a) Local education agencies shall have bulk samples, collected under § 763.86 and submitted for analysis, analyzed for asbestos using laboratories...

Preliminary Phytochemical Evaluation of Seed Extracts of Cucurbita Maxima Duchense

OpenAIRE

Ashok Sharma; Ashish K. Sharma; Tara Chand; Manoj Khardiya; Kailash Chand Yadav

2013-01-01

Cucurbita maxima Duchense (family:Cucurbitaceae) is a trailing annual herb, widely cultivated throughout India and in most warm regions of the world, for used as vegetables as well as medicines. The present study deals with preliminary physicochemical and phytochemical evaluation of seed extract of Cucurbita maxima. The study includes preparation of different extracts by successive solvent extraction for detailed analysis. Different physicochemical parameters such as ash value (total ash, aci...

Quantitative evaluation of fMRI retinotopic maps, from V1 to V4, for cognitive experiments

Directory of Open Access Journals (Sweden)

Cecile eBordier

2015-05-01

Full Text Available FMRI retinotopic mapping is a non-invasive technique for the delineation of low-level visual areas in individual subjects. It generally relies upon the analysis of functional responses to periodic visual stimuli that encode eccentricity or polar angle in the visual field. This technique is used in vision research when the precise assignation of brain activation to retinotopic areas is an issue. It involves processing steps computed with different algorithms and embedded in various software suites. Manual intervention may be needed for some steps. Although the diversity of the available processing suites and manual interventions may potentially introduce some differences in the final delineation of visual areas, no documented comparison between maps obtained with different procedures has been reported in the literature. To explore the effect of the processing steps on the quality of the maps obtained, we used two tools, BALC, which relies on a fully automated procedure, and BrainVoyager, where areas are delineated by hand on the brain surface. To focus on the mapping procedures specifically, we used the same SPM pipeline for pretreatment and the same tissue segmentation tool. We document the consistency and differences of the fMRI retinotopic maps obtained from routine retinotopy experiments on ten subjects. The maps obtained by skilled users are never fully identical. However, the agreement between the maps, around 80% for low-level areas, is probably sufficient for most applications. Our results also indicate that assigning cognitive activations, following a specific experiment (here, color perception, to individual retinotopic maps is not free of errors. We provide measurements of this error, that may help for the cautious interpretation of cognitive activation projection onto fMRI retinotopic maps. On average, the magnitude of the error is about 20%, with much larger differences in a few subjects.

Preliminary evaluation of sweet spot size in virtual sound reproduction using dipoles

DEFF Research Database (Denmark)

Lacouture Parodi, Yesenia; Rubak, Per

2009-01-01

to the loudspeakers. In this paper we present a follow up evaluation of the performance of the three inversion techniques when these conditions are violated. A setup to measure the sweet spot of different loudspeakers arrangements is described. Preliminary measurement results are presented for loudspeakers placed...

Susceptibilities of enterovirus D68, enterovirus 71, and rhinovirus 87 strains to various antiviral compounds.

Science.gov (United States)

Smee, Donald F; Evans, W Joseph; Nicolaou, K C; Tarbet, E Bart; Day, Craig W

2016-07-01

Compounds were evaluated for antiviral activity in rhabdomyosarcoma (RD) cells against a recent 2014 clinical isolate of enterovirus D68 (EV-D68), a 1962 strain of EV-68D, rhinovirus 87 (RV-87, serologically the same as EV-D68), and enterovirus 71 (EV-71). Test substances included known-active antipicornavirus agents (enviroxime, guanidine HCl, pirodavir, pleconaril, and rupintrivir), nucleobase/nucleoside analogs (3-deazaguanine and ribavirin), and three novel epidithiodiketopiperazines (KCN-2,2'-epi-19, KCN-19, and KCN-21). Of these, rupintrivir was the most potent, with 50% inhibition of viral cytopathic effect (EC50) and 90% inhibition (EC90) of virus yield at 0.0022-0.0053 μM against EV-D68. Enviroxime, pleconaril and the KCN compounds showed efficacy at 0.01-0.3 μM; 3-deazaguanine and pirodavir inhibited EV-D68 at 7-13 μM, and guanidine HCl and ribavirin were inhibitory at 80-135 μM. Pirodavir was active against EV-71 (EC50 of 0.78 μM) but not against RV-87 or EV-D68, and all other compounds were less effective against EV-71 than against RV-87 and EV-D68. The most promising compound inhibiting both virus infections at low concentrations was rupintrivir. Antiviral activity was confirmed for the ten compounds in virus yield reduction (VYR) assays in RD cells, and for enviroxime, guanidine HCl, and pirodavir by cytopathic effect (CPE) assays in A549, HeLa-Ohio-1, and RD cells. These studies may serve as a basis for further pre-clinical discovery of anti-enterovirus inhibitors. Furthermore, the antiviral profiles and growth characteristics observed herein support the assertion that EV-D68 should be classified together with RV-87. Copyright © 2016 Elsevier B.V. All rights reserved.

Evaluation of GMI and PMI diffeomorphic‐based demons algorithms for aligning PET and CT Images

Science.gov (United States)

Yang, Juan; Zhang, You; Yin, Yong

2015-01-01

Fusion of anatomic information in computed tomography (CT) and functional information in F18‐FDG positron emission tomography (PET) is crucial for accurate differentiation of tumor from benign masses, designing radiotherapy treatment plan and staging of cancer. Although current PET and CT images can be acquired from combined F18‐FDG PET/CT scanner, the two acquisitions are scanned separately and take a long time, which may induce potential positional errors in global and local caused by respiratory motion or organ peristalsis. So registration (alignment) of whole‐body PET and CT images is a prerequisite for their meaningful fusion. The purpose of this study was to assess the performance of two multimodal registration algorithms for aligning PET and CT images. The proposed gradient of mutual information (GMI)‐based demons algorithm, which incorporated the GMI between two images as an external force to facilitate the alignment, was compared with the point‐wise mutual information (PMI) diffeomorphic‐based demons algorithm whose external force was modified by replacing the image intensity difference in diffeomorphic demons algorithm with the PMI to make it appropriate for multimodal image registration. Eight patients with esophageal cancer(s) were enrolled in this IRB‐approved study. Whole‐body PET and CT images were acquired from a combined F18‐FDG PET/CT scanner for each patient. The modified Hausdorff distance (dMH) was used to evaluate the registration accuracy of the two algorithms. Of all patients, the mean values and standard deviations (SDs) of dMH were 6.65 (± 1.90) voxels and 6.01 (± 1.90) after the GMI‐based demons and the PMI diffeomorphic‐based demons registration algorithms respectively. Preliminary results on oncological patients showed that the respiratory motion and organ peristalsis in PET/CT esophageal images could not be neglected, although a combined F18‐FDG PET/CT scanner was used for image acquisition. The PMI

A mutação JAK2 V617F e as síndromes mieloproliferativas JAK2 V617F mutation and the myeloproliferative disorders

Directory of Open Access Journals (Sweden)

Bárbara C. R. Monte-Mór

2008-01-01

Full Text Available Síndromes mieloproliferativas (SMPs são doenças hematopoéticas de origem clonal que apresentam amplificação de uma ou mais linhagens mielóides. Policitemia vera (PV, trombocitemia essencial (TE, mielofibrose idiopática (MF e leucemia mielóide crônica (LMC são consideradas SMPs clássicas e apresentam características clínicas e biológicas comuns. Ao contrário de LMC, cuja etiologia está relacionada à proteína constitutivamente ativa Bcr-Abl, o mecanismo molecular de PV, TE e MF permaneceu por muito tempo desconhecido. Esta revisão se foca na recente descoberta da mutação JAK2 V617F em pacientes com PV, TE e MF, sua relação com o fenótipo mieloproliferativo e implicações na abordagem clínica de pacientes.Myeloproliferative disorders are clonal hematopoietic diseases that are characterized by the amplification of one or more myeloid lineages. Polycythemia vera, essential thrombocythemia, idiopathic myelofibrosis and chronic myeloid leukemia are considered classic myeloproliferative disorders and share common clinical and biological features. While the genetic basis of chronic myeloid leukemia is shown to be the constitutive active protein BCR-ABL, the main molecular lesions in polycythemia vera, essential thrombocythemia and idiopathic myelofibrosis remain unknown. This review focuses on the recent discovery of the JAK2 V617F mutation, its relationship to the myeloproliferative phenotype and implications in the clinical approach of patients.

Treatment response evaluation with 18F-FDG PET/CT and 18F-NaF PET/CT in multiple myeloma patients undergoing high-dose chemotherapy and autologous stem cell transplantation.

Science.gov (United States)

Sachpekidis, Christos; Hillengass, J; Goldschmidt, H; Wagner, B; Haberkorn, U; Kopka, K; Dimitrakopoulou-Strauss, A

2017-01-01

The aim of this study was to assess the combined use of the radiotracers 18 F-FDG and 18 F-NaF in treatment response evaluation of a group of multiple myeloma (MM) patients undergoing high-dose chemotherapy (HDT) followed by autologous stem cell transplantation (ASCT) by means of static (whole-body) and dynamic PET/CT (dPET/CT). Thirty-four patients with primary, previously untreated MM scheduled for treatment with HDT followed by ASCT were enrolled in the study. All patients underwent PET/CT scanning with 18 F-FDG and 18 F-NaF before and after therapy. Treatment response by means of PET/CT was assessed according to the European Organization for Research and Treatment of Cancer (EORTC) 1999 criteria. The evaluation of dPET/CT studies was based on qualitative evaluation, semi-quantitative (SUV) calculation, and quantitative analysis based on two-tissue compartment modelling and a non-compartmental approach leading to the extraction of fractal dimension (FD). An analysis was possible in 29 patients: three with clinical complete response (CR) and 26 with non-CR (13 patients near complete response-nCR, four patients very good partial response-VGPR, nine patients partial response-PR). After treatment, 18 F-FDG PET/CT was negative in 14/29 patients and positive in 15/29 patients, showing a sensitivity of 57.5 % and a specificity of 100 %. According to the EORTC 1999 criteria, 18 F-FDG PET/CT-based treatment response revealed CR in 14 patients ( 18 F-FDG PET/CT CR), PR in 11 patients ( 18 F-FDG PET/CT PR) and progressive disease in four patients ( 18 F-FDG PET/CT PD). In terms of 18 F-NaF PET/CT, 4/29 patients (13.8 %) had a negative baseline scan, thus failed to depict MM. Regarding the patients for which a direct lesion-to-lesion comparison was feasible, 18 F-NaF PET/CT depicted 56 of the 129 18 F-FDG positive lesions (43 %). Follow-up 18 F-NaF PET/CT showed persistence of 81.5 % of the baseline 18 F-NaF positive MM lesions after treatment, despite the fact that 64

Swedish Hard Rock Laboratory first evaluation of preinvestigations 1986-87 and target area characterization

International Nuclear Information System (INIS)

Gustafson, G.; Stanfors, R.; Wikberg, P.

1988-06-01

SKB plans to site an underground research laboratory in the Simpevarp area. A regional survey started in 1986 and an extensive programme for geology, geohydrology and hydrochemistry was carried through. This report gives an evaluation of all available data gathered from the start of the project up to the drilling of core boreholes in some target areas in the autumn of 1987. A descriptive geological-tectonic model on a regional scale is presented that is intended to constitute a basis for the hydrogeological modelling work. Preliminary rock mass descriptions are also presented on a more detailed scale for some minor parts of the area. It is recommended that the island Aespoe is the principal target area for the continued work on the Swedish Hard Rock Laboratory. (orig.)

22 CFR 8.7 - Security.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Security. 8.7 Section 8.7 Foreign Relations DEPARTMENT OF STATE GENERAL ADVISORY COMMITTEE MANAGEMENT § 8.7 Security. (a) All officers and members of a committee must have a security clearance for the subject matter level of security at which the committee...

33 CFR 8.7 - Information.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Information. 8.7 Section 8.7... GUARD RESERVE § 8.7 Information. (a) Information concerning the Coast Guard Reserve may be obtained from Commandant (CG-13), 2100 2nd St. SW., Stop 7801, Washington, DC 20593-7801. (b) Information and requirements...

24 CFR 87.105 - Definitions.

Science.gov (United States)

2010-04-01

... services in the private sector. (o) Recipient includes all contractors, subcontractors at any tier, and... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Definitions. 87.105 Section 87.105... RESTRICTIONS ON LOBBYING General § 87.105 Definitions. For purposes of this part: (a) Agency, as defined in 5 U...

Laser frequency locking based on the normal and abnormal saturated absorption spectroscopy of 87Rb

International Nuclear Information System (INIS)

Wan Jian-Hong; Liu Chang; Wang Yan-Hui

2016-01-01

We present a practical method to avoid the mis-locking phenomenon in the saturated-absorption-spectrum laser-frequency-locking system and set up a simple theoretical model to explain the abnormal saturated absorption spectrum. The method uses the normal and abnormal saturated absorption spectra of the same transition 5 2 S 1/2 , F = 2–5 2 P 3/2 , F′ = 3 saturated absorption of the 87 Rb D 2 resonance line. After subtracting these two signals with the help of electronics, we can obtain a spectrum with a single peak to lock the laser. In our experiment, we use the normal and inverse signals of the transitions 5 2 S 1/2 , F = 2–5 2 P 3/2 , F′ = 3 saturated absorption of the 87 Rb D 2 resonance line to lock a 780-nm distributed feedback (DFB) diode laser. This method improves the long-term locking performance and is suitable for other kinds of diode lasers. (paper)

Den komplexa väven Att organisera för barns övergångar till och från förskoleklass

Directory of Open Access Journals (Sweden)

Helena Ackesjö

2015-09-01

Full Text Available This study focuses on how children's transitions to and from preschool classes are organized in different schools. Principal’s arguments for this organization were sought via a web-based survey and analyzed using the frame factor theory in addition to theories of practical sense and the concept of continuity. The results show that children tend to make more transitions between social communities the younger they are. The distribution of the principal’s answers show that the majority of them, because of external conditions, split the preschool groups to new classes in the transition to preschool class. However, the same movements are not indicated in the transition to first grade. The results show how the work of organizing chidren’s transitions represent a complex web of external actual conditions as the number of children and the recruitment area of the school, the internal logics and ideas about what is best for children and best for continued learning as well as the school's own traditions of working with transitions. Föreliggande studie fokuserar på hur barns övergångar till och från förskoleklass organiseras på olika skolor. Via en webbaserad enkät söks skolledares argument för denna organisering. I analysen nyttjas ett ramfaktorteoretiskt tänkande som kompletteras med teorier om praktiskt förnuft samt kontinuitetsbegreppet. Resultaten visar att barn tenderar att göra fler övergångar mellan barngrupper och sociala gemenskaper ju yngre de är. Fördelningen av skolledarnas svar visar att majoriteten, på grund av yttre villkor, delar förskolegrupperna till nya klasser i övergången till förskoleklass. Däremot indikeras inte samma rörlighet i övergången till årskurs 1. Resultaten visar hur arbetet med att organisera övergångarna utgör en komplex väv av yttre faktiska villkor som barnantal och upptagningsområdets storlek, av inre logiker och föreställningar om vad som är det bästa för barnen och det b

EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2014. Scientific Opinion on Flavouring Group Evaluation 87, Revision 2 (FGE.87Rev2): Consideration of bicyclic secondary alcohols, ketones and related esters evaluated by JECFA (63rd meeting, ) structurally related to bicyclic secondary alcohols, ketones and related esters evaluated by EFSA in FGE.47Rev1 (2008)

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Frandsen, Henrik Lauritz; Nørby, Karin Kristiane

evaluation is necessary, as laid down in Commission Regulation (EC) No 1565/2000. The present consideration concerns a group of 19 bicyclic secondary alcohols, ketones and related esters evaluated by the JECFA at the 63rd meeting in 2004. This revision of FGE.87 is made due to inclusion of two additional...

Bristande samsyn i lärares definition av hot och våld : En kvalitativ studie om förekomst av hot och våld mot lärare på högstadieskolor.

OpenAIRE

Eklund, Anton; Johansson, Tommy

2017-01-01

Denna kvalitativa studie syftar till att studera förekomsten av hot och våld mot lärare på högstadieskolor, hur man på de undersökta skolorna arbetar med att minimera förekomsten av hot och våld samt hur lärare definierar sin syn på vad som kan vara hot och våld från föräldrar och elever. Studien är baserad på ett riktat bekvämlighetsurval där skyddsombud på högstadieskolor i den undersökta kommunen valdes ut. I samband med analysen av studiens resultat har Lintons rollteori använts för att s...

RsmV a small non-coding regulatory RNA in Pseudomonas aeruginosa that sequesters RsmA and RsmF from target mRNAs.

Science.gov (United States)

Janssen, Kayley H; Diaz, Manisha R; Gode, Cindy J; Wolfgang, Matthew C; Yahr, Timothy L

2018-06-04

The Gram-negative opportunistic pathogen Pseudomonas aeruginosa has distinct genetic programs that favor either acute or chronic virulence gene expression. Acute virulence is associated with twitching and swimming motility, expression of a type III secretion system (T3SS), and the absence of alginate, Psl, or Pel polysaccharide production. Traits associated with chronic infection include growth as a biofilm, reduced motility, and expression of a type VI secretion system (T6SS). The Rsm post-transcriptional regulatory system plays important roles in the inverse control of phenotypes associated with acute and chronic virulence. RsmA and RsmF are RNA-binding proteins that interact with target mRNAs to control gene expression at the post-transcriptional level. Previous work found that RsmA activity is controlled by at least three small, non-coding regulatory RNAs (RsmW, RsmY, and RsmZ). In this study, we took an in-silico approach to identify additional sRNAs that might function in the sequestration of RsmA and/or RsmF and identified RsmV, a 192 nt transcript with four predicted RsmA/RsmF consensus binding sites. RsmV is capable of sequestering RsmA and RsmF in vivo to activate translation of tssA1 , a component of the T6SS, and to inhibit T3SS gene expression. Each of the predicted RsmA/RsmF consensus binding sites contribute to RsmV activity. Electrophoretic mobility shifts assays show that RsmF binds RsmV with >10-fold higher affinity than RsmY and RsmZ. Gene expression studies revealed that the temporal expression pattern of RsmV differs from RsmW, RsmY, and RsmZ. These findings suggest that each sRNA may play distinct roles in controlling RsmA and RsmF activity. IMPORTANCE The CsrA/RsmA family of RNA-binding proteins play important roles in post-transcriptional control of gene expression. The activity of CsrA/RsmA proteins is controlled by small non-coding RNAs that function as decoys to sequester CsrA/RsmA from target mRNAs. Pseudomonas aeruginosa has two Csr

Production and decay of the F-meson in e+e- annihilation at 10 GeV centre-of-mass energy

International Nuclear Information System (INIS)

Albrecht, H.; Binder, U.; Drews, G.; Harder, G.; Hasemann, H.; Philipp, A.; Schmidt-Parzefall, W.; Schroeder, H.; Schulz, H.D.; Selonke, F.; Wurth, R.; Drescher, A.; Graewe, B.; Hofmann, W.; Markees, A.; Matthiesen, U.; Scheck, H.; Spengler, J.; Wegener, D.; Edwards, K.W.; Yun, J.C.; Frisken, W.R.; Fukunaga, C.; Goddard, M.; Gilkinson, D.J.; Gingrich, D.M.; Kim, P.C.H.; Kutschke, R.; MacFarlane, D.B.; McKenna, J.A.; Orr, R.S.; Padley, P.; Prentice, J.D.; Seywerd, H.C.J.; Stacey, B.J.; Yoon, T.S.; Ammar, R.; Coppage, D.; Davis, R.; Kanekal, S.; Kwak, N.; Boeckmann, P.; Joensson, L.; Oku, Y.; Childers, R.; Darden, C.W.; Gennow, H.

1984-12-01

Using the ARGUS detector at DORIS, we have observed the production of Fsup(+-) mesons in e + e - annihilation at a centre of mass energy of 10 GeV through their subsequent decays into PHIπsup(+-) and PHIπ + π - πsup(+-). The values obtained for [R(e + e - -> F x). Branching Ratio] are (1.47 +- 0.32 +- 0.20)% and (1.63 +- 0.42 +- 0.41)% respectively. The observed mass is (1973.6 +- 2.6 +- 3.0) MeV/c 2 . The F momentum spectrum is as expected for the fragmentation of c quarks into charmed mesons, but is somewhat softer than for fragmentation into Dsup(*) mesons. The relevant angular distributions are consistent with a spin zero assignment of the F meson. (orig.)

The evaluation and calculation of production cross sections of {sup 18}F, {sup 77}Br and {sup 186}Re medical radioisotopes from {sup 18}O, {sup 77}Se and {sup 186}W(p,n) reactions up to 80 MeV

Energy Technology Data Exchange (ETDEWEB)

Youxiang, Zhuang [Chinese Nuclear Data Center, Beijing, BJ (China)

1996-06-01

The medical radioisotopes are used for diagnostic and therapeutic purposes, as well as metabolism and physiological function researches in modern medicine. The major applications are of functional imaging using PET agents for {sup 18}F, various therapeutic pharmaceuticals via auger electrons for {sup 77}Br, cancers diagnosis and therapy for {sup 186}Re. The evaluations of experimental data and theoretical calculations for {sup 18}O, {sup 77}Se, {sup 187}W(p,n) reactions up to 80 MeV are presented and analysed. The recommended values for {sup 18}O(p,n){sup 18}F, {sup 77}Se(p,n){sup 77}Br and {sup 186}W(p,n){sup 186}Re reaction cross sections are given. (6 figs.).

Synthesis and preliminary evaluation of 18F-labeled 4-thia palmitate as a PET tracer of myocardial fatty acid oxidation

International Nuclear Information System (INIS)

DeGrado, Timothy R.; Wang Shuyan; Holden, James E.; Nickles, R. Jerome; Taylor, Michael; Stone, Charles K.

2000-01-01

Interest remains strong for the development of a noninvasive technique for assessment of regional fatty acid oxidation rate in the myocardium. 18 F-labeled 4-thia palmitate (FTP, 16-[ 18 F]fluoro-4-thia-hexadecanoic acid) has been synthesized and preliminarily evaluated as a metabolically trapped probe of myocardial fatty acid oxidation for positron emission tomography (PET). The radiotracer is synthesized by Kryptofix 2.2.2/K 2 CO 3 assisted nucleophilic radiofluorination of an iodo-ester precursor, followed by alkaline hydrolysis and by purification by reverse phase high performance liquid chromatography. Biodistribution studies in rats showed high uptake and long retention of FTP in heart, liver, and kidneys consistent with relatively high fatty acid oxidation rates in these tissues. Inhibition of carnitine palmitoyl-transferase-I caused an 80% reduction in myocardial uptake, suggesting the dependence of trapping on the transport of tracer into the mitochondrion. Experiments with perfused rat hearts showed that the estimates of the fractional metabolic trapping rate (FR) of FTP tracked inhibition of oxidation rate of palmitate with hypoxia, whereas the FR of the 6-thia analog 17-[ 18 F]fluoro-6-thia-heptadecanoic acid was insensitive to hypoxia. In vivo defluorination of FTP in the rat was evidenced by bone uptake of radioactivity. A PET imaging study with FTP in normal swine showed excellent myocardial images, prolonged myocardial retention, and no bone uptake of radioactivity up to 3 h, the last finding suggesting a species dependence for defluorination of the omega-labeled fatty acid. The results support further investigation of FTP as a potential PET tracer for assessing regional fatty acid oxidation rate in the human myocardium

17F breakup reactions: a touchstone for indirect measurements

International Nuclear Information System (INIS)

De Napoli, M.; Raciti, G.; Sfienti, C.; Capel, P.; Baye, D.; Descouvemont, P.; Sparenberg, J.-M.; Giacoppo, F.; Rapisarda, E.; Cardella, G.; Mazzocchi, C.

2011-01-01

An exclusive study of 17 F breakup reactions has been performed at the FRIBs facility of the Laboratori Nazionali del Sud in Catania (Italy). The experiment has been performed with the aim of testing the accuracy of the Coulomb-breakup indirect technique used to infer radiative-capture cross sections at low energies. This technique has been used in the 7 Be(p,γ) 8 B case, but has never been tested. By measuring the breakup of 17 F into 16 O+p, and comparing the inferred cross section for 16 O(p,γ) 17 F to direct precise measurements, the influence of E2 transitions and higher-order effects, that are predicted to be significant in Coulomb-breakup reactions, can be evaluated. The first results and preliminary model comparison are reported.

JAK2 V617F, MPL, and CALR mutations in essential thrombocythaemia and major thrombotic complications: a single-institute retrospective analysis.

Science.gov (United States)

Pósfai, Éva; Marton, Imelda; Király, Péter Attila; Kotosz, Balázs; Kiss-László, Zsuzsanna; Széll, Márta; Borbényi, Zita

2015-07-01

Thrombo-haemorrhagic events are the main cause of morbidity and mortality in essential thrombocythemia. The aim of this study was to estimate the incidence of thrombotic events and the impact of the JAK2V617F, MPL (W515L, W515K, W515R, W515A and S505N) and CALR (type-1, type-2) mutations on 101 essential thrombocythaemia patients (72 females and 29 males with a mean age of 61 years) diagnosed in a Southern Hungarian regional academic centre. The incidence of major thrombosis was 13.86 %. Sixty percent of the patients carried the JAK2V617F mutation. The MPL mutations were analysed by sequencing and the W515L was the only one we could identify with an incidence of 3.96 %. Type-2 CALR mutation could be identified in 3 cases among the patients who had JAK2/MPL-unmutated ET. Statistical analyses revealed that the JAK2V617F mutation was associated with significantly increased levels of platelet (p = 0.042), haemoglobin (p = 0.000), red blood cell (p = 0.000) and haematocrit (p = 0.000) and hepatomegaly (p = 0.045) at diagnosis compared to JAK2V617F negative counterparts, however there was no significant association between the JAK2V617F mutation status (relative risk: 1.297, 95 % CI 0.395-4.258; p = 0.668) and subsequent thrombotic complications. The impact of JAK2V617F, MPL W515L and CALR mutations on the clinical findings at the diagnosis of ET was obvious, but their statistically significant role in the prediction of thrombotic events could not be proven in this study. Our results indirectly support the concept that, besides the quantitative and qualitative changes in the platelets, the mechanisms leading to thrombosis are more complex and multifactorial.

Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion.

Science.gov (United States)

Kent, David G; Li, Juan; Tanna, Hinal; Fink, Juergen; Kirschner, Kristina; Pask, Dean C; Silber, Yvonne; Hamilton, Tina L; Sneade, Rachel; Simons, Benjamin D; Green, Anthony R

2013-01-01

Recent descriptions of significant heterogeneity in normal stem cells and cancers have altered our understanding of tumorigenesis, emphasizing the need to understand how single stem cells are subverted to cause tumors. Human myeloproliferative neoplasms (MPNs) are thought to reflect transformation of a hematopoietic stem cell (HSC) and the majority harbor an acquired V617F mutation in the JAK2 tyrosine kinase, making them a paradigm for studying the early stages of tumor establishment and progression. The consequences of activating tyrosine kinase mutations for stem and progenitor cell behavior are unclear. In this article, we identify a distinct cellular mechanism operative in stem cells. By using conditional knock-in mice, we show that the HSC defect resulting from expression of heterozygous human JAK2V617F is both quantitative (reduced HSC numbers) and qualitative (lineage biases and reduced self-renewal per HSC). The defect is intrinsic to individual HSCs and their progeny are skewed toward proliferation and differentiation as evidenced by single cell and transplantation assays. Aged JAK2V617F show a more pronounced defect as assessed by transplantation, but mice that transform reacquire competitive self-renewal ability. Quantitative analysis of HSC-derived clones was used to model the fate choices of normal and JAK2-mutant HSCs and indicates that JAK2V617F reduces self-renewal of individual HSCs but leaves progenitor expansion intact. This conclusion is supported by paired daughter cell analyses, which indicate that JAK2-mutant HSCs more often give rise to two differentiated daughter cells. Together these data suggest that acquisition of JAK2V617F alone is insufficient for clonal expansion and disease progression and causes eventual HSC exhaustion. Moreover, our results show that clonal expansion of progenitor cells provides a window in which collaborating mutations can accumulate to drive disease progression. Characterizing the mechanism(s) of JAK2V617F

TG101209, a small molecule JAK2-selective kinase inhibitor potently inhibits myeloproliferative disorder-associated JAK2V617F and MPLW515L/K mutations.

Science.gov (United States)

Pardanani, A; Hood, J; Lasho, T; Levine, R L; Martin, M B; Noronha, G; Finke, C; Mak, C C; Mesa, R; Zhu, H; Soll, R; Gilliland, D G; Tefferi, A

2007-08-01

JAK2V617F and MPLW515L/K represent recently identified mutations in myeloproliferative disorders (MPD) that cause dysregulated JAK-STAT signaling, which is implicated in MPD pathogenesis. We developed TG101209, an orally bioavailable small molecule that potently inhibits JAK2 (IC(50)=6 nM), FLT3 (IC(50)=25 nM) and RET (IC(50)=17 nM) kinases, with significantly less activity against other tyrosine kinases including JAK3 (IC(50)=169 nM). TG101209 inhibited growth of Ba/F3 cells expressing JAK2V617F or MPLW515L mutations with an IC(50) of approximately 200 nM. In a human JAK2V617F-expressing acute myeloid leukemia cell line, TG101209-induced cell cycle arrest and apoptosis, and inhibited phosphorylation of JAK2V617F, STAT5 and STAT3. Therapeutic efficacy of TG101209 was demonstrated in a nude mouse model. Furthermore, TG101209 suppressed growth of hematopoietic colonies from primary progenitor cells harboring JAK2V617F or MPL515 mutations.

Should an obsessive-compulsive spectrum grouping of disorders be included in DSM-V?

Science.gov (United States)

Phillips, Katharine A; Stein, Dan J; Rauch, Scott L; Hollander, Eric; Fallon, Brian A; Barsky, Arthur; Fineberg, Naomi; Mataix-Cols, David; Ferrão, Ygor Arzeno; Saxena, Sanjaya; Wilhelm, Sabine; Kelly, Megan M; Clark, Lee Anna; Pinto, Anthony; Bienvenu, O Joseph; Farrow, Joanne; Leckman, James

2010-06-01

The obsessive-compulsive (OC) spectrum has been discussed in the literature for two decades. Proponents of this concept propose that certain disorders characterized by repetitive thoughts and/or behaviors are related to obsessive-compulsive disorder (OCD), and suggest that such disorders be grouped together in the same category (i.e. grouping, or "chapter") in DSM. This article addresses this topic and presents options and preliminary recommendations to be considered for DSM-V. The article builds upon and extends prior reviews of this topic that were prepared for and discussed at a DSM-V Research Planning Conference on Obsessive-Compulsive Spectrum Disorders held in 2006. Our preliminary recommendation is that an OC-spectrum grouping of disorders be included in DSM-V. Furthermore, we preliminarily recommend that consideration be given to including this group of disorders within a larger supraordinate category of "Anxiety and Obsessive-Compulsive Spectrum Disorders." These preliminary recommendations must be evaluated in light of recommendations for, and constraints upon, the overall structure of DSM-V. (c) 2010 Wiley-Liss, Inc.

Hanford Waste Vitrification Plant technical manual

Energy Technology Data Exchange (ETDEWEB)

Larson, D.E. [ed.; Watrous, R.A.; Kruger, O.L. [and others

1996-03-01

A key element of the Hanford waste management strategy is the construction of a new facility, the Hanford Waste Vitrification Plant (HWVP), to vitrify existing and future liquid high-level waste produced by defense activities at the Hanford Site. The HWVP mission is to vitrify pretreated waste in borosilicate glass, cast the glass into stainless steel canisters, and store the canisters at the Hanford Site until they are shipped to a federal geological repository. The HWVP Technical Manual (Manual) documents the technical bases of the current HWVP process and provides a physical description of the related equipment and the plant. The immediate purpose of the document is to provide the technical bases for preparation of project baseline documents that will be used to direct the Title 1 and Title 2 design by the A/E, Fluor. The content of the Manual is organized in the following manner. Chapter 1.0 contains the background and context within which the HWVP was designed. Chapter 2.0 describes the site, plant, equipment and supporting services and provides the context for application of the process information in the Manual. Chapter 3.0 provides plant feed and product requirements, which are primary process bases for plant operation. Chapter 4.0 summarizes the technology for each plant process. Chapter 5.0 describes the engineering principles for designing major types of HWVP equipment. Chapter 6.0 describes the general safety aspects of the plant and process to assist in safe and prudent facility operation. Chapter 7.0 includes a description of the waste form qualification program and data. Chapter 8.0 indicates the current status of quality assurance requirements for the Manual. The Appendices provide data that are too extensive to be placed in the main text, such as extensive tables and sets of figures. The Manual is a revision of the 1987 version.

Hanford Waste Vitrification Plant technical manual

International Nuclear Information System (INIS)

Larson, D.E.; Watrous, R.A.; Kruger, O.L.

1996-03-01

A key element of the Hanford waste management strategy is the construction of a new facility, the Hanford Waste Vitrification Plant (HWVP), to vitrify existing and future liquid high-level waste produced by defense activities at the Hanford Site. The HWVP mission is to vitrify pretreated waste in borosilicate glass, cast the glass into stainless steel canisters, and store the canisters at the Hanford Site until they are shipped to a federal geological repository. The HWVP Technical Manual (Manual) documents the technical bases of the current HWVP process and provides a physical description of the related equipment and the plant. The immediate purpose of the document is to provide the technical bases for preparation of project baseline documents that will be used to direct the Title 1 and Title 2 design by the A/E, Fluor. The content of the Manual is organized in the following manner. Chapter 1.0 contains the background and context within which the HWVP was designed. Chapter 2.0 describes the site, plant, equipment and supporting services and provides the context for application of the process information in the Manual. Chapter 3.0 provides plant feed and product requirements, which are primary process bases for plant operation. Chapter 4.0 summarizes the technology for each plant process. Chapter 5.0 describes the engineering principles for designing major types of HWVP equipment. Chapter 6.0 describes the general safety aspects of the plant and process to assist in safe and prudent facility operation. Chapter 7.0 includes a description of the waste form qualification program and data. Chapter 8.0 indicates the current status of quality assurance requirements for the Manual. The Appendices provide data that are too extensive to be placed in the main text, such as extensive tables and sets of figures. The Manual is a revision of the 1987 version

Quality control of a kV cone beam computed tomography imaging system

International Nuclear Information System (INIS)

Marguet, M.; Bodez, V.

2009-01-01

Purpose: This work presents the introduction of a quality assurance program for the On-Board Imager (O.B.I., Varian) kV cone beam computed tomography (kV C.B.C.T.) system, together with the results of 1 year monthly testing. Materials and methods: Firstly the geometric precision and stability of the equipment and of the associated software were evaluated using the Marker phantom. The coincidence of the accelerator isocenter and the imager isocenter was verified as well as the accuracy of the registration of kV cone beam computed tomography (kV C.B.C.T.) with reference CT images. Then, the kV C.B.C.T. image quality was evaluated using the Catphan 504 phantom and ArtiScan software (Aquilab) for both full-fan (F.F.) and half-fan (H.F.) imaging modes. Results: The kV C.B.C.T. isocenter and image registration with correction of the table position were found to be within a tolerance of 2.0 mm. Concerning the kV C.B.C.T. image quality, image noise and uniformity, the Hounsfield units (HU) stability and linearity, geometric distortion and high contrast resolution were all found to be within the manufacturer's recommendations for both F.F. and H.F. modes. However, the low contrast resolution for the HF mode did not meet the manufacturer's specifications. Conclusion: The quality assurance tests introduced have defined the initial system characteristics and their evolution during a period of 1 year, demonstrating the stability of the O.B.I.. (authors)

Finite-element model evaluation of barrier configurations to reduce infiltration into waste-disposal structures: preliminary results and design considerations

International Nuclear Information System (INIS)

Lu, A.H.; Phillips, S.J.; Adams, M.R.

1982-09-01

Barriers to reduce infiltration into waste burial disposal structures (trenches, pits, etc.) may be required to provide adequate waste confinement. The preliminary engineering design of these barriers should consider interrelated barrier performance factors. This paper summarizes preliminary computer simulation activities to further engineering barrier design efforts. Several barrier configurations were conceived and evaluated. Models were simulated for each barrier configuration using a finite element computer code. Results of this preliminary evaluation indicate that barrier configurations, depending on their morphology and materials, may significantly influence infiltration, flux, drainage, and storage of water through and within waste disposal structures. 9 figures

Human Rhinovirus 87 and Enterovirus 68 Represent a Unique Serotype with Rhinovirus and Enterovirus Features

Science.gov (United States)

Blomqvist, Soile; Savolainen, Carita; Råman, Laura; Roivainen, Merja; Hovi, Tapani

2002-01-01

It has recently been reported that all but one of the 102 known serotypes of the genus Rhinovirus segregate into two genetic clusters (C. Savolainen, S. Blomqvist, M. N. Mulders, and T. Hovi, J. Gen. Virol. 83:333-340, 2002). The only exception is human rhinovirus 87 (HRV87). Here we demonstrate that HRV87 is genetically and antigenically highly similar to enterovirus 68 (EV68) and is related to EV70, the other member of human enterovirus group D. The partial nucleotide sequences of the 5′ untranslated region, capsid regions VP4/VP2 and VP1, and the 3D RNA polymerase gene of the HRV87 prototype strain F02-3607 Corn showed 97.3, 97.8, 95.2, and 95.9% identity to the corresponding regions of EV68 prototype strain Fermon. The amino acid identities were 100 and 98.1% for the products of the two capsid regions and 97.9% for 3D RNA polymerase. Antigenic cross-reaction between HRV87 and EV68 was indicated by microneutralization with monotypic antisera. Phylogenetic analysis showed definite clustering of HRV87 and EV68 with EV70 for all sequences examined. Both HRV87 and EV68 were shown to be acid sensitive by two different assays, while EV70 was acid resistant, which is typical of enteroviruses. The cytopathic effect induced by HRV87 or EV68 was inhibited by monoclonal antibodies to the decay-accelerating factor known to be the receptor of EV70. We conclude that HRV87 and EV68 are strains of the same picornavirus serotype presenting features of both rhinoviruses and enteroviruses. PMID:12409401

Laste füüsiline väärkohtlemine versus karistamine ja tagajärjed : [bakalaureusetöö] / Marianne Ausen ; Tartu Ülikool, õigusteaduskond ; juhendaja: Silvia Kaugia

Index Scriptorium Estoniae

Ausen, Marianne, 1976-

2013-01-01

Empiirilise uuringu läbiviimisest ja valimist, lapse väärkohtlemise mõistest, vormidest ja subjektidest, lapse füüsilise väärkohtlemise tunnustest, karistamisest ja väärkohtlemisest, füüsilise väärkohtlemise tagajärgedest

Relationship of JAK2V617F gene mutation with cell proliferation and coagulation function in myeloproliferative neoplasms

Directory of Open Access Journals (Sweden)

Xiao-Nan Zhang

2017-05-01

Full Text Available Objective: To study the relationship of JAK2V617F gene mutation with cell proliferation and coagulation function in myeloproliferative neoplasms. Methods: Patients who were diagnosed with BCR-ABL-negative myeloproliferative neoplasms in Anyang District Hospital between June 2014 and August 2016 were selected, JAK2V617F gene mutation was detected, and according to the test results, the patients were divided into mutation-positive group and mutation-negative group. The expression of JAK2/STATs signaling pathway molecules and cell proliferation genes in bone marrow fluid as well as the coagulation function indexes in peripheral blood were detected. Results: p-JAK2, p-STAT3, p-STAT5, Survivin, C-myc, CyclinD1 and ASXL1 protein expression in myeloproliferative neoplasms of mutation-positive group were significantly higher than those of mutation-negative group, and peripheral blood PT and APTT levels were significantly lower than those of mutation-negative group while TT and FIB levels were not significantly different from those of mutation-negative group. Conclusion: JAK2V617F gene mutation in myeloproliferative neoplasms can promote the cell proliferation and cause the hypercoagulable state.

Preliminary Evaluation of Method to Monitor Landfills Resilience against Methane Emission

Science.gov (United States)

Chusna, Noor Amalia; Maryono, Maryono

2018-02-01

Methane emission from landfill sites contribute to global warming and un-proper methane treatment can pose an explosion hazard. Stakeholder and government in the cities in Indonesia been found significant difficulties to monitor the resilience of landfill from methane emission. Moreover, the management of methane gas has always been a challenging issue for long waste management service and operations. Landfills are a significant contributor to anthropogenic methane emissions. This study conducted preliminary evaluation of method to manage methane gas emission by assessing LandGem and IPCC method. From the preliminary evaluation, this study found that the IPCC method is based on the availability of current and historical country specific data regarding the waste disposed of in landfills while from the LandGEM method is an automated tool for estimating emission rates for total landfill gas this method account total gas of methane, carbon dioxide and other. The method can be used either with specific data to estimate emissions in the site or default parameters if no site-specific data are available. Both of method could be utilize to monitor the methane emission from landfill site in cities of Central Java.

SU-F-T-204: A Preliminary Approach of Reducing Contralateral Breast and Heart Dose in Left Sided Whole Breast Cancer Patients Utilizing Proton Beams

Energy Technology Data Exchange (ETDEWEB)

Islam, M; Algan, O; Jin, H; Ahmad, S; Hossain, S [University of Oklahoma Health Sciences Center, Oklahoma City, OK (United States)

2016-06-15

Purpose: To investigate the plan quality and feasibility of a hybrid plan utilizing proton and photon fields for superior coverage in the internal mammary (IM) and supraclavicular (S/C) regions while minimizing heart and contralateral breast dose for the left-sided whole breast cancer patient treatment. Methods: This preliminary study carried out on single left-sided intact breast patient involved IM and S/C nodes. The IM and S/C node fields of the 5-Field 3DCRT photon-electron base plan were replaced by two proton fields. These two along with two Field-in-Field tangential photon fields were optimized for comparable dose coverage. The treatment plans were done using Eclipse TPS for the total dose of 46Gy in 23 fractions with 95% of the prescription dose covering 95% of the RTOG PTV. The 3DCRT photon-electron and 4-Field photon-proton hybrid plans were compared for the PTV dose coverage as well as dose to OARs. Results: The overall RTOG PTV coverage for proton-hybrid and 3DCRT plan was comparable (95% of prescription dose covers 95% PTV volume). In proton-hybrid plan, 99% of IM volume received 100% dose whereas in 3DCRT only 77% received 100% dose. For S/C regions, 97% and 77% volume received 100% prescription dose in proton-hybrid and 3DCRT plans, respectively. The heart mean dose, V3Gy(%), and V5Gy(%) was 2.2Gy, 14.4%, 9.8% for proton-hybrid vs. 4.20 Gy, 21.5%, and 39% for 3DCRT plan, respectively. The maximum dose to the contralateral breast was 39.75Gy for proton-hybrid while 56.87Gy for 3DCRT plan. The mean total lung dose, V20Gy(%), and V30Gy(%) was 5.68Gy, 11.3%, 10.5% for proton-hybrid vs. 5.90Gy, 9.8%, 7.2% for 3DCRT, respectively. Conclusion: The protonhybrid plan can offer better dose coverage to the involved lymphatic tissues while lower doses to the heart and contralateral breast. More treatment plans are currently in progress before being implemented clinically.

Limited efficacy of hydroxyurea in lowering of the JAK2 V617F allele burden

DEFF Research Database (Denmark)

Larsen, Thomas Stauffer; Pallisgaard, Niels; de Stricker, Karin

2009-01-01

Besides being an invaluable marker of clonal disease in chronic myeloproliferative disorders (CMPDs), the JAK2 V617F mutation and the mutated allele burden have an impact on disease phenotype and may provide information on prognosis. Recently, hydroxyurea (HU) has been shown to induce a rapid...

Quantitative assessment of multi-isocentric radiosurgical irradiation based on the f factor

International Nuclear Information System (INIS)

Lefkopoulos, D.; Schlienger, M.; Hancilar, T.; Keraudy, K.; Touboul, E.

1995-01-01

To evaluate quantitatively the stereotactic irradiations, we defined a DVH parameter (f) resulting from the lesion underdosage and healthy tissues overdosage curves. The Lesion Underdose Factor (LUF) is the ratio of the lesion volume receiving dose inferior to a reference isodose to the lesion volume. The Healthy Tissue Overdose Factor HTOF quantitates the amount of tissue external to the lesion receiving dose equal to or greater than a given reference isodose relatively to the lesion volume. The LUF and HTOF curves represent the variation of the LUF and HTOF factors as a function of the reference isodose. The f factor is the surface situated below the intersection of the two curves. The optimization goal of the planification procedure could consist in the minimization of f. We have correlated them with clinical results (obliteration of the nidus) of a series of 34 multi-isocenters AVMs linac irradiation. The treatment dose protocol was a peripheral dose of 20 - 25 Gy (50 to 70% isodose range). According to this preliminary study, it seems that f factor provides clinically useful quantitative indices for the treatment plan evaluation. The choice of the optimal treatment plan and reference isodose is realized as follows. For a same patient different treatment plans are calculated by successive modifications of the irradiation parameters, as the number of isocenters, the distances between isocenters, the collimators diameter, the number of arcs and their angular length etc. The choice of optimal treatment plan is based on a clinically validated quantitative factor such as the f factor. According to our preliminary results one should select the treatment plan corresponding to the smallest value of f. The choice of the optimal reference isodose is based on the clinical data (lesion volume and topography, adjacent critical structures) and the LUF and HTOF factors following a well-defined protocol

Energy measurement of prompt fission neutrons in 239Pu(n,f) for incident neutron energies from 1 to 200 MeV

CERN Document Server

Chatillon, A; Granier, Th; Laurent, B; Taïeb, J; Noda, S; Haight, R C; Devlin, M; Nelson, R O; O’Donnell, J M

2010-01-01

Prompt fission neutron spectra in the neutron-induced fission of 239Pu have been measured for incident neutron energies from 1 to 200 MeV at the Los Alamos Neutron Science Center. Preliminary results are discussed and compared to theoretical model calculation.

Cosmology based on f(R) gravity with O(1) eV sterile neutrino

Energy Technology Data Exchange (ETDEWEB)

Chudaykin, Anton S.; Gorbunov, Dmitry S. [Institute for Nuclear Research of the Russian Academy of Sciences, 60th October Anniversary prospect 7a, Moscow 117312 (Russian Federation); Starobinsky, Alexei A. [L.D. Landau Institute for Theoretical Physics of the Russian Academy of Sciences, Moscow 119334 (Russian Federation); Burenin, Rodion A., E-mail: chudy@ms2.inr.ac.ru, E-mail: gorby@ms2.inr.ac.ru, E-mail: alstar@landau.ac.ru, E-mail: rodion@hea.iki.rssi.ru [Space Research Institute of the Russian Academy of Sciences (IKI), Moscow, ul. Profsoyuznaya, 84/32, 117997 (Russian Federation)

2015-05-01

We address the cosmological role of an additional O(1) eV sterile neutrino in modified gravity models. We confront the present cosmological data with predictions of the FLRW cosmological model based on a variant of f(R) modified gravity proposed by one of the authors previously. This viable cosmological model which deviation from general relativity with a cosmological constant Λ decreases as R{sup −2n} for large, but not too large values of the Ricci scalar R (while no Λ is introduced by hand at small R) provides an alternative explanation of present dark energy and the accelerated expansion of the Universe (the case n=2 is considered in the paper). Various up-to-date cosmological data sets exploited include measurements of the cosmic microwave background (CMB) anisotropy, the CMB lensing potential, the baryon acoustic oscillations (BAO), the cluster mass function and the Hubble constant. We find that the CMB+BAO constraints strongly restrict the sum of neutrino masses from above. This excludes values of the model parameter λ∼ 1 for which distinctive cosmological features of the model are mostly pronounced as compared to the ΛCDM model, since then free streaming damping of perturbations due to neutrino rest masses is not sufficient to compensate their extra growth occurring in f(R) modified gravity. Thus, in the gravity sector we obtain λ>8.2 (2σ) with the account of systematic uncertainties in galaxy cluster mass function measurements and λ>9.4 (2σ) without them. At the same time in the latter case we find for the sterile neutrino mass 0.47 eV < m{sub ν, sterile} < 1 eV (2σ) assuming that the sterile neutrinos are thermalized and the active neutrinos are massless, not significantly larger than in the standard ΛCDM with the same data set: 0.45 eV < m{sub ν, sterile} < 0.92 eV (2σ). However, a possible discovery of a sterile neutrino with the mass m{sub ν, sterile} ≈ 1.5 eV motivated by various anomalies in neutrino oscillation

Evaluation of a flow direction probe and a pitot-static probe on the F-14 airplane at high angles of attack and sideslip

Science.gov (United States)

Larson, T. J.

1984-01-01

The measurement performance of a hemispherical flow-angularity probe and a fuselage-mounted pitot-static probe was evaluated at high flow angles as part of a test program on an F-14 airplane. These evaluations were performed using a calibrated pitot-static noseboom equipped with vanes for reference flow direction measurements, and another probe incorporating vanes but mounted on a pod under the fuselage nose. Data are presented for angles of attack up to 63, angles of sideslip from -22 deg to 22 deg, and for Mach numbers from approximately 0.3 to 1.3. During maneuvering flight, the hemispherical flow-angularity probe exhibited flow angle errors that exceeded 2 deg. Pressure measurements with the pitot-static probe resulted in very inaccurate data above a Mach number of 0.87 and exhibited large sensitivities with flow angle.

Preclinical evaluation and quantification of [18F]MK-9470 as a radioligand for PET imaging of the type 1 cannabinoid receptor in rat brain

International Nuclear Information System (INIS)

Casteels, Cindy; Koole, Michel; Laere, Koen van; Celen, Sofie; Bormans, Guy

2012-01-01

[ 18 F]MK-9470 is an inverse agonist for the type 1 cannabinoid (CB1) receptor allowing its use in PET imaging. We characterized the kinetics of [ 18 F]MK-9470 and evaluated its ability to quantify CB1 receptor availability in the rat brain. Dynamic small-animal PET scans with [ 18 F]MK-9470 were performed in Wistar rats on a FOCUS-220 system for up to 10 h. Both plasma and perfused brain homogenates were analysed using HPLC to quantify radiometabolites. Displacement and blocking experiments were done using cold MK-9470 and another inverse agonist, SR141716A. The distribution volume (V T ) of [ 18 F]MK-9470 was used as a quantitative measure and compared to the use of brain uptake, expressed as SUV, a simplified method of quantification. The percentage of intact [ 18 F]MK-9470 in arterial plasma samples was 80 ± 23 % at 10 min, 38 ± 30 % at 40 min and 13 ± 14 % at 210 min. A polar radiometabolite fraction was detected in plasma and brain tissue. The brain radiometabolite concentration was uniform across the whole brain. Displacement and pretreatment studies showed that 56 % of the tracer binding was specific and reversible. V T values obtained with a one-tissue compartment model plus constrained radiometabolite input had good identifiability (≤10 %). Ignoring the radiometabolite contribution using a one-tissue compartment model alone, i.e. without constrained radiometabolite input, overestimated the [ 18 F]MK-9470 V T , but was correlated. A correlation between [ 18 F]MK-9470 V T and SUV in the brain was also found (R 2 = 0.26-0.33; p ≤ 0.03). While the presence of a brain-penetrating radiometabolite fraction complicates the quantification of [ 18 F]MK-9470 in the rat brain, its tracer kinetics can be modelled using a one-tissue compartment model with and without constrained radiometabolite input. (orig.)

Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis

Directory of Open Access Journals (Sweden)

Tognon Raquel

2012-02-01

Full Text Available Abstract Background Essential Thrombocythemia (ET and Primary Myelofibrosis (PMF are Chronic Myeloproliferative Neoplasms (MPN characterized by clonal myeloproliferation/myeloaccumulation without cell maturation impairment. The JAK2 V617F mutation and PRV1 gene overexpression may contribute to MPN physiopathology. We hypothesized that deregulation of the apoptotic machinery may also play a role in the pathogenesis of ET and PMF. In this study we evaluated the apoptosis-related gene and protein expression of BCL2 family members in bone marrow CD34+ hematopoietic stem cells (HSC and peripheral blood leukocytes from ET and PMF patients. We also tested whether the gene expression results were correlated with JAK2 V617F allele burden percentage, PRV1 overexpression, and clinical and laboratory parameters. Results By real time PCR assay, we observed that A1, MCL1, BIK and BID, as well as A1, BCLW and BAK gene expression were increased in ET and PMF CD34+ cells respectively, while pro-apoptotic BAX and anti-apoptotic BCL2 mRNA levels were found to be lower in ET and PMF CD34+ cells respectively, in relation to controls. In patients' leukocytes, we detected an upregulation of anti-apoptotic genes A1, BCL2, BCL-XL and BCLW. In contrast, pro-apoptotic BID and BIMEL expression were downregulated in ET leukocytes. Increased BCL-XL protein expression in PMF leukocytes and decreased BID protein expression in ET leukocytes were observed by Western Blot. In ET leukocytes, we found a correlation between JAK2 V617F allele burden and BAX, BIK and BAD gene expression and between A1, BAX and BIK and PRV1 gene expression. A negative correlation between PRV1 gene expression and platelet count was observed, as well as a positive correlation between PRV1 gene expression and splenomegaly. Conclusions Our results suggest the participation of intrinsic apoptosis pathway in the MPN physiopathology. In addition, PRV1 and JAK2 V617F allele burden were linked to deregulation

Analyzing power in inclusive. pi. sup + and. pi. sup - production at high x sub F with a 200 GeV polarized proton beam

Energy Technology Data Exchange (ETDEWEB)

Adams, D L; Bonner, B E; Buchanan, J A; Clement, J M; Corcoran, M D; Cranshaw, J; Nessi-Tedaldi, F; Nessi, M; Nguyen, C; Phillips, G C; Roberts, J B; Skeens, J; White, J L [T.W. Bonner Nuclear Lab., Rice Univ., Houston, TX (United States); Akchurin, N; Langland, J; Onel, Y [Dept. of Physics, Univ. of Iowa, Iowa City, IA (United States); Belikov, N I; Derevschikov, A A; Grachov, O A; Matulenko, Yu A; Meschanin, A P; Nurushev, S B; Patalakha, D I; Rykov, V L; Solovyanov, V L; Vasiliev, A N [Inst. of High Energy Physics, Serpukhov (USSR); Bystricky, J; Lehar, F; Lesquen, A de [CEN-Saclay, 91 - Gif-sur-Yvette (France); Cossairt, J D; Read, A L [Fermi National Accelerator Lab., Batavia, IL (United States); En' yo, H; Funahashi, H; Goto, Y; Imai, K; Itow, Y; Makino, S; Masaike, A; Miyake, K; Nagamine, T; Saito, N; Yamashita, S [Dept. of Physics, Kyoto Univ. (Japan); Grosnick, D P; Hill, D A; Lopiano, D; Ohashi, Y; Spinka, H; Stanek, R; FNAL E704 Collaboration

1991-08-01

The analyzing power in inclusive charged pion production has been measured using the 200 GeV Fermilab polarized proton beam. A striking dependence in {chi}{sub F} is observed in which A{sub N} increases from 0 to 0.42 with increasing {chi}{sub F} for the {pi}{sup +} data and decreases from 0 to -0.38 with increasing {chi}{sub F} for {pi}{sup -} data. The kinematic range covered is 0.2{<=}{chi}{sub F}{<=}0.9 and 0.2{<=}p{sub T}{<=}2.0 GeV/c. In a simple model our data indicate that at large {chi}{sub F} the transverse spin of the proton is correlated with that of its quark constituents. (orig.).

Fuzzy comprehensive evaluation method of F statistics weighting in ...

African Journals Online (AJOL)

In order to rapidly identify the source of water inrush in coal mine, and provide the theoretical basis for mine water damage prevention and control, fuzzy comprehensive evaluation model was established. The F statistics of water samples was normalized as the weight of fuzzy comprehensive evaluation for determining the ...

Extending Jak2V617F and MplW515 mutation analysis to single hematopoietic colonies and B and T lymphocytes.

Science.gov (United States)

Pardanani, Animesh; Lasho, Terra L; Finke, Christy; Mesa, Ruben A; Hogan, William J; Ketterling, Rhett P; Gilliland, Dwight Gary; Tefferi, Ayalew

2007-09-01

JAK2V617F and MPLW515L/K are myeloproliferative disorder (MPD)-associated mutations. We genotyped 552 individual hematopoietic colonies obtained by CD34+ cell culture from 16 affected patients (13 JAK2V617F and 3 MPLW515L/K) to determine (a) the proportion of colonies harboring a particular mutation in the presence or absence of cytokines, (b) the lineage distribution of endogenous colonies for each mutation, and (c) the differences (if any) in the pattern of mutation among the various MPDs, as established by genotyping of individual colonies. Genotyping analysis revealed cohabitation of mutation-negative and mutation-positive endogenous colonies in polycythemia vera as well as other MPDs. Culture of progenitor cells harboring MPLW515L/K yielded virtually no endogenous erythroid colonies in contrast to JAK2V617F-harboring progenitor cells. The mutation pattern (i.e., relative distribution of homozygous, heterozygous, or wild-type colonies) was not a distinguishing feature among the MPDs, and MPLW515 mutations were detected in B and/or T lymphocytes in all three patients tested. These observations suggest that clonal myelopoiesis antedates acquisition of JAK2V617F or MPLW515L/K mutations and that the latter is acquired in a lympho-myeloid progenitor cell.

Hanford Waste Vitrification Plant

International Nuclear Information System (INIS)

Larson, D.E.; Allen, C.R.; Kruger, O.L.; Weber, E.T.

1991-10-01

The Hanford Waste Vitrification Plant (HWVP) is being designed to immobilize pretreated Hanford high-level waste and transuranic waste in borosilicate glass contained in stainless steel canisters. Testing is being conducted in the HWVP Technology Development Project to ensure that adapted technologies are applicable to the candidate Hanford wastes and to generate information for waste form qualification. Empirical modeling is being conducted to define a glass composition range consistent with process and waste form qualification requirements. Laboratory studies are conducted to determine process stream properties, characterize the redox chemistry of the melter feed as a basis for controlling melt foaming and evaluate zeolite sorption materials for process waste treatment. Pilot-scale tests have been performed with simulated melter feed to access filtration for solids removal from process wastes, evaluate vitrification process performance and assess offgas equipment performance. Process equipment construction materials are being selected based on literature review, corrosion testing, and performance in pilot-scale testing. 3 figs., 6 tabs

Color tuning in alert macaque V1 assessed with fMRI and single-unit recording shows a bias toward daylight colors.

Science.gov (United States)

Lafer-Sousa, Rosa; Liu, Yang O; Lafer-Sousa, Luis; Wiest, Michael C; Conway, Bevil R

2012-05-01

Colors defined by the two intermediate directions in color space, "orange-cyan" and "lime-magenta," elicit the same spatiotemporal average response from the two cardinal chromatic channels in the lateral geniculate nucleus (LGN). While we found LGN functional magnetic resonance imaging (fMRI) responses to these pairs of colors were statistically indistinguishable, primary visual cortex (V1) fMRI responses were stronger to orange-cyan. Moreover, linear combinations of single-cell responses to cone-isolating stimuli of V1 cone-opponent cells also yielded stronger predicted responses to orange-cyan over lime-magenta, suggesting these neurons underlie the fMRI result. These observations are consistent with the hypothesis that V1 recombines LGN signals into "higher-order" mechanisms tuned to noncardinal color directions. In light of work showing that natural images and daylight samples are biased toward orange-cyan, our findings further suggest that V1 is adapted to daylight. V1, especially double-opponent cells, may function to extract spatial information from color boundaries correlated with scene-structure cues, such as shadows lit by ambient blue sky juxtaposed with surfaces reflecting sunshine. © 2012 Optical Society of America

Detection of MPL exon10 mutations in 103 Chinese patients with JAK2V617F-negative myeloproliferative neoplasms.

Science.gov (United States)

Chen, Xiuhua; Qi, Xiling; Tan, Yanhong; Xu, Zhifang; Xu, Aining; Zhang, Linlin; Wang, Hongwei

2011-06-15

JAK2V617F mutation has been reported in 90% of patients with polycythemia vera (PV) and about 50% of patients with essential thromobocythemia (ET) and primary myelofibrosis (PMF). Recently, acquired mutations in the transmembrane-juxtamembrane region of MPL (MPLW515 mutations) have been reported in approximately 5% of JAK2V617F-negative PMF and about 1% of all cases of ET. MPL is the receptor for thrombopoietin that regulates the production of platelets by bone marrow. It is likely that some mutations more closely related to ET in MPL exon10 may have been missed by current assays. We inferred that there might be other mutations in MPL exon10 for MPN patients in addition to MPLW515 mutations. To investigate its mutation types and prevalence in Chinese patients with myeloproliferative neoplasms (MPN), we performed mutation detection on MPL exon10 in 103 JAK2V617F-negative MPN patients by single strand conformation polymorphism (SSCP) and allele-specific PCR (AS-PCR) combined with sequencing. As a result, one previously unrecognized MPL mutation (12-bp in-frame insertion) was identified in one patient with ET in addition to an MPLW515K mutation identified in one PMF patient. This confirms our hypothesis that BCR/ABL negative and JAK2V617F-negative MPN patients have other mutations besides W515 mutation in MPL exon10 and mutations other than single nucleotide exchange also exist. In addition, MPL mutation was associated with Chinese MPN patients. Copyright © 2011 Elsevier Inc. All rights reserved.

Treating Substance-Using Women and Their Children in Public Housing: Preliminary Evaluation Findings.

Science.gov (United States)

Metsch, Lisa R.; Wolfe, Harlan P.; Fewell, Rebecca; McCoy, Clyde B.; Elwood, William N.; Wohler-Torres, Brad; Petersen-Baston, Pamela; Haskins, Henry V.

2001-01-01

SafePort is a residential substance abuse treatment program within public housing to provide drug treatment to parenting women in Key West, Florida. All family members--women, children, and significant others--receive comprehensive assessments to determine appropriate therapeutic interventions. Preliminary evaluation findings suggest that women…

Imaging of alpha(v)beta(3) expression by a bifunctional chimeric RGD peptide not cross-reacting with alpha(v)beta(5).

Science.gov (United States)

Zannetti, Antonella; Del Vecchio, Silvana; Iommelli, Francesca; Del Gatto, Annarita; De Luca, Stefania; Zaccaro, Laura; Papaccioli, Angela; Sommella, Jvana; Panico, Mariarosaria; Speranza, Antonio; Grieco, Paolo; Novellino, Ettore; Saviano, Michele; Pedone, Carlo; Salvatore, Marco

2009-08-15

To test whether a novel bifunctional chimeric peptide comprising a cyclic Arg-Gly-Asp pentapeptide covalently bound to an echistatin domain can discriminate alpha(v)beta(3) from alpha(v)beta(5) integrin, thus allowing the in vivo selective visualization of alpha(v)beta(3) expression by single-photon and positron emission tomography (PET) imaging. The chimeric peptide was preliminarily tested for inhibition of alpha(v)beta(3)-dependent cell adhesion and competition of 125I-echistatin binding to membrane of stably transfected K562 cells expressing alpha(v)beta(3) (Kalpha(v)beta(3)) or alpha(v)beta(5) (Kalpha(v)beta(5)) integrin. The chimeric peptide was then conjugated with diethylenetriaminepentaacetic acid and labeled with 111In for single-photon imaging, whereas a one-step procedure was used for labeling the full-length peptide and a truncated derivative, lacking the last five C-terminal amino acids, with 18F for PET imaging. Nude mice bearing tumors from Kalpha(v)beta(3), Kalpha(v)beta(5), U87MG human glioblastoma, and A431 human epidermoid cells were subjected to single-photon and PET imaging. Adhesion and competitive binding assays showed that the novel chimeric peptide selectively binds to alpha(v)beta(3) integrin and does not cross-react with alpha(v)beta(5). In agreement with in vitro findings, single-photon and PET imaging studies showed that the radiolabeled chimeric peptide selectively localizes in tumor xenografts expressing alphavbeta3 and fails to accumulate in those expressing alpha(v)beta(5) integrin. When 18F-labeled truncated derivative was used for PET imaging, alphavbeta3- and alpha(v)beta(5)-expressing tumors were visualized, indicating that the five C-terminal amino acids are required to differentially bind the two integrins. Our findings indicate that the novel chimeric Arg-Gly-Asp peptide, having no cross-reaction with alphavbeta5 integrin, allows highly selective alphavbeta3 expression imaging and monitoring.

Establishing optimal quantitative-polymerase chain reaction assays for routine diagnosis and tracking of minimal residual disease in JAK2-V617F-associated myeloproliferative neoplasms: a joint European LeukemiaNet/MPN&MPNr-EuroNet (COST action BM0902) study.

Science.gov (United States)

Jovanovic, J V; Ivey, A; Vannucchi, A M; Lippert, E; Oppliger Leibundgut, E; Cassinat, B; Pallisgaard, N; Maroc, N; Hermouet, S; Nickless, G; Guglielmelli, P; van der Reijden, B A; Jansen, J H; Alpermann, T; Schnittger, S; Bench, A; Tobal, K; Wilkins, B; Cuthill, K; McLornan, D; Yeoman, K; Akiki, S; Bryon, J; Jeffries, S; Jones, A; Percy, M J; Schwemmers, S; Gruender, A; Kelley, T W; Reading, S; Pancrazzi, A; McMullin, M F; Pahl, H L; Cross, N C P; Harrison, C N; Prchal, J T; Chomienne, C; Kiladjian, J J; Barbui, T; Grimwade, D

2013-10-01

Reliable detection of JAK2-V617F is critical for accurate diagnosis of myeloproliferative neoplasms (MPNs); in addition, sensitive mutation-specific assays can be applied to monitor disease response. However, there has been no consistent approach to JAK2-V617F detection, with assays varying markedly in performance, affecting clinical utility. Therefore, we established a network of 12 laboratories from seven countries to systematically evaluate nine different DNA-based quantitative PCR (qPCR) assays, including those in widespread clinical use. Seven quality control rounds involving over 21,500 qPCR reactions were undertaken using centrally distributed cell line dilutions and plasmid controls. The two best-performing assays were tested on normal blood samples (n=100) to evaluate assay specificity, followed by analysis of serial samples from 28 patients transplanted for JAK2-V617F-positive disease. The most sensitive assay, which performed consistently across a range of qPCR platforms, predicted outcome following transplant, with the mutant allele detected a median of 22 weeks (range 6-85 weeks) before relapse. Four of seven patients achieved molecular remission following donor lymphocyte infusion, indicative of a graft vs MPN effect. This study has established a robust, reliable assay for sensitive JAK2-V617F detection, suitable for assessing response in clinical trials, predicting outcome and guiding management of patients undergoing allogeneic transplant.

Preliminary radiation shielding design for BOOMERANG

International Nuclear Information System (INIS)

Donahue, Richard J.

2002-01-01

Preliminary radiation shielding specifications are presented here for the 3 GeV BOOMERANG Australian synchrotron light source project. At this time the bulk shield walls for the storage ring and injection system (100 MeV Linac and 3 GeV Booster) are considered for siting purposes