WorldWideScience

Sample records for human carrying capacity

  1. Carrying Capacity

    DEFF Research Database (Denmark)

    Schroll, Henning; Andersen, Jan; Kjærgård, Bente

    2012-01-01

    A spatial planning act was introduced inIndonesia 1992 and renewed in 2008. It emphasised the planning role of decentralised authorities. The spatial planning act covers both spatial and environmental issues. It defines the concept of carrying capacity and includes definitions of supportive....../cities. Four different sectors (water, food production, waste, and forests) were selected as core areas for decentralised spatial planning. Indicators for SCC and ACC were identified and assessed with regard to relevance and quantifiability. For each of the indicators selected, a legal threshold or guiding...... was introduced inIndonesia 1992 and renewed in 2008. It emphasised the planning role of decentralised authorities. The spatial planning act covers both spatial and environmental issues. It defines the concept of carrying capacity and includes definitions of supportive carrying capacity (SCC) and assimilative...

  2. The Concept of Carrying Capacity in Tourism

    Directory of Open Access Journals (Sweden)

    Josef Zelenka

    2014-05-01

    Full Text Available Carrying capacity is often pragmatically, theoretically as well as purely intuitively considered as a concept in the context of tourism sustainability. The carrying capacity application has the greatest potential in protected areas, in frequently visited cultural and natural attractions, and in relation to sustaining of the lifestyle of the local community and tourism destination potential in general. Despite its importance, partial applications, determination of basic theoretical principles, and specifying connection to the other theoretical concepts in tourism (particularly destination life cycle, LAC concept, visitors management, there still is a rightful opinion of some authors suggesting that there is no consistent theory of tourism carrying capacity. This theory would be the base for sophisticated practical carrying capacity applications. This paper is therefore focused on introduction of the theoretical concept of carrying capacity, which can be discussed and possibly further elaborated.

  3. Measuring Social carrying Capacity: An Exploratory Study

    OpenAIRE

    López-Bonilla, Jesús Manuel; López-Bonilla, Luis Miguel

    2007-01-01

    The tourist carrying capacity commands a growing interest given that it is closely linked with sustainable tourist development. The justification of the utility of this concept is given by means of a simple and efficient methodological proposal, by analysing the social carrying capacity. To this end, an empirical application is carried out in the Western Andalusia. In some of the cases analysed, the satisfaction of the tourist is found to decline when the levels of the tourist use are higher ...

  4. Carrying the past to the future: Distinct brain networks underlie individual differences in human spatial working memory capacity.

    Science.gov (United States)

    Liu, Siwei; Poh, Jia-Hou; Koh, Hui Li; Ng, Kwun Kei; Loke, Yng Miin; Lim, Joseph Kai Wei; Chong, Joanna Su Xian; Zhou, Juan

    2018-08-01

    Spatial working memory (SWM) relies on the interplay of anatomically separated and interconnected large-scale brain networks. EEG studies often observe load-associated sustained negative activity during SWM retention. Yet, whether and how such sustained negative activity in retention relates to network-specific functional activation/deactivation and relates to individual differences in SWM capacity remain to be elucidated. To cover these gaps, we recorded concurrent EEG-fMRI data in 70 healthy young adults during the Sternberg delayed-match-to-sample SWM task with three memory load levels. To a subset of participants (N = 28) that performed the task properly and had artefact-free fMRI and EEG data, we employed a novel temporo-spatial principal component analysis to derive load-dependent negative slow wave (NSW) from retention-related event-related potentials. The associations between NSW responses with SWM capacity were divergent in the higher (N = 14) and lower (N = 14) SWM capacity groups. Specifically, larger load-related increase in NSW amplitude was associated with greater SWM capacity for the higher capacity group but lower SWM capacity for the lower capacity group. Furthermore, for the higher capacity group, larger NSW amplitude was related to greater activation in bilateral parietal areas of the fronto-parietal network (FPN) and greater deactivation in medial frontal gyrus and posterior mid-cingulate cortex of the default mode network (DMN) during retention. In contrast, the lower capacity group did not show similar pattern. Instead, greater NSW was linked to higher deactivation in right posterior middle temporal gyrus. Our findings shed light on the possible differential EEG-informed neural network mechanism during memory maintenance underlying individual differences in SWM capacity. Copyright © 2018 Elsevier Inc. All rights reserved.

  5. Rapid Population Growth and Human Carrying Capacity: Two Perspectives. World Bank Staff Working Papers No. 690 and Population and Development Series No. 15.

    Science.gov (United States)

    Mahar, Dennis J., Ed.; And Others

    Two perspectives on carrying capacity and population growth are examined. The first perspective, "Carrying Capacity and Rapid Population Growth: Definition, Cases, and Consequences" (Robert Muscat), explores the possible meanings of the idea of carrying capacity under developing country conditions, looks at historical and present-day cases of…

  6. Carrying capacity: the tradition and policy implications of limits

    Directory of Open Access Journals (Sweden)

    Virginia Deane Abernethy

    2001-01-01

    Full Text Available ABSTRACT: Within just the last few centuries, science and technology have enlarged human capabilities and population size until humans now take, for their own use, nearly half of the Earth's net terrestrial primary production. An ethical perspective suggests that potentials to alter, or further increase, humanity's use of global resources should be scrutinized through the lenses of self-interested foresightedness and respect for non-human life. Without overtly invoking ethics, studies of the carrying capacity achieve just this objective. Carrying capacity is an ecological concept that expresses the relationship between a population and the natural environment on which it depends for ongoing sustenance. Carrying capacity assumes limits on the number of individuals that can be supported at a given level of consumption without degrading the environment and, therefore, reducing future carrying capacity. That is, carrying capacity addresses long-term sustainability. Worldviews differ in the importance accorded to the carrying capacity concept. This paper addresses three worldviews - ecological, romantic, and entrepreneurial - and explores the ethics and the policy implications of their contrasting perspectives.

  7. Research on Water Resources Design Carrying Capacity

    Directory of Open Access Journals (Sweden)

    Guanghua Qin

    2016-04-01

    Full Text Available Water resources carrying capacity (WRCC is a recently proposed management concept, which aims to support sustainable socio-economic development in a region or basin. However, the calculation of future WRCC is not well considered in most studies, because water resources and the socio-economic development mode for one area or city in the future are quite uncertain. This paper focused on the limits of traditional methods of WRCC and proposed a new concept, water resources design carrying capacity (WRDCC, which incorporated the concept of design. In WRDCC, the population size that the local water resources can support is calculated based on the balance of water supply and water consumption, under the design water supply and design socio-economic development mode. The WRDCC of Chengdu city in China is calculated. Results show that the WRDCC (population size of Chengdu city in development modeI (II, III will be 997 ×104 (770 × 104, 504 × 104 in 2020, and 934 × 104 (759 × 104, 462 × 104 in 2030. Comparing the actual population to the carrying population (WRDCC in 2020 and 2030, a bigger gap will appear, which means there will be more and more pressure on the society-economic sustainable development.

  8. Estimating the recreational carrying capacity of a lowland river section.

    Science.gov (United States)

    Lorenz, Stefan; Pusch, Martin T

    2012-01-01

    Recreational boating represents a major human use of inland waters in many regions. However, boating tourism may affect the ecological integrity of surface waters in multiple ways. In particular, surface waves produced by boating may disturb freshwater invertebrates, such as interrupting the filtration activity of benthic mussels. As mussels may significantly contribute to self-purification, disturbance may have crucial impacts on water quality, and thus on water tourism. In this paper we calculate the carrying capacity of a river section for sustainable boating tourism based on the preservation of water quality. This approach is complemented by spatial and social approaches for carrying capacity estimates. The ecological carrying capacity significantly decreases with lower water levels during summer. Hence, the analysis of variables that influence the river's carrying capacity allows the formation of recommendations for management measures that integrate social, touristic and ecological aspects.

  9. Evaluation of carrying capacity and territorial environmental sustainability

    Directory of Open Access Journals (Sweden)

    Giuseppe Ruggiero

    2012-09-01

    Full Text Available Land use has a great impact on environmental quality, use of resources, state of ecosystems and socio-economic development. Land use can be considered sustainable if the environmental pressures of human activities do not exceed the ecological carrying capacity. A scientific knowledge of the capability of ecosystems to provide resources and absorb waste is a useful and innovative means of supporting territorial planning. This study examines the area of the Province of Bari to estimate the ecosystems’ carrying capacity, and compare it with the current environmental pressures exerted by human activities. The adapted methodology identified the environmentally sustainable level for one province.

  10. Research on Psychological Carrying Capacity of Tourism Destination

    Institute of Scientific and Technical Information of China (English)

    Fan Zhiyong; Zhong Sheng

    2009-01-01

    As a part of the carrying capacity system of tourism destination,tourism psychological carrying capacity and its makeup are very important indexes which reflect the harmonious development of tourism destination develops harmoniously,but the academy has not paid enough attention to them.Based on the concept and connotation of psychological carrying capacity,this paper explains the influencing factors which affect the psychological capacity of the tourist and the resident after the acknowledged concept,and then designs a harmonious development model of tourism destination.Finally,it offers some countermeasures against the overloading psychological capacity.

  11. The Population Growth and Carrying Capacity in Semarang City

    Science.gov (United States)

    Hariyanto; Hadi, Sudharto P.; Buchori, Imam

    2018-02-01

    Population growth and development of city activities take some lands to carry them. As a result, land use competition happens among persons, society or sector. Land necessity for settlement, industry, or sector has taken over farm land, therefore farm land has been converted intensively and massively. Chronologically, population growth will cause land necessity increase. Unproductive land, especially farm land will be converted. Furthermore, farm land conversion will cause carrying capacity change. Carrying capacity has certain bio capacity. With the population growth, it will increase resource consumption; on the other side, farm land conversion will decrease carrying capacity. The objective of the study is to know about the influence of population growth towards carrying capacity (bio capacity) in Semarang city. Land consumption per capita is indeed influenced by city population, the higher the population is, the lower the land consumption per capita. With the population growth, it will influence carrying capacity. Carrying capacity here is the ratio of area to population. Analytical descriptive method is applied in the study with all sub-districts in Semarang city as the analysis unit. Population here is sub-district area and population per sub-district in Semarang city. Population growth data period is from 2000 until 2015. Main variables of the study are area per sub-district, population, population growth, carrying capacity. Result of the study shows significant influence of carrying capacity decrease, especially some outskirts in Semarang city. This condition happens because the outskirts in Semarang city tend to have dense population growth. Range of carrying capacity in Semarang city is from 0,007 to 0,117 of 0 to 1. Almost all sub-districts in Semarang city show miserable condition, except Mijen and Tugu. The conclusion of the study is that population will decrease carrying capacity. Therefore, the government should control population growth by paying

  12. Evaluating total carrying capacity of tourism using impact indicators

    Directory of Open Access Journals (Sweden)

    R. Sharma

    2016-03-01

    Full Text Available The carrying capacity is well identified tool to manage problems due to uncontrolled tourism for any destination. This report highlights the carrying capacity estimation of Kerwa tourism area, Bhopal, India. The methodology used in this report is a new two-tier mechanism of impact analysis using index numbers derived from a survey of 123 stakeholders. From this the individual component impact analysis and the total carrying capacity of the area is computed in order to state the insight of the total carrying capacity left for the tourism activities in Kerwa tourism area. It is calculated from, the results so obtained, that the Kerwa catchment area falls in “very low impact category” and hence in a healthy state of the artwork in terms of total carrying capacity. The study conveys the current need in the destination management and tourism development as a road map for the destination managers for implementing sustainable tourism.

  13. The flexible application of carrying capacity in ecology

    Directory of Open Access Journals (Sweden)

    Eric J. Chapman

    2018-01-01

    Full Text Available Carrying capacity encompasses a broad collection of approaches used to better understand biotic interactions in ecosystems and is often applied with no explicit regard to its historical origin. In this paper, we reviewed the primary literature to examine how carrying capacity is applied in ecology. We focused our review on ecosystem studies—studies that frame their results at the ecosystem level—published after the 1950s and highlight emerging trends of this concept. We found that while carrying capacity offers some underlying commonalities, a wide range of definitions and approaches hinders a unified framework to better understand biotic ecosystem interactions. Not surprisingly, these studies most often use K—the number of individuals that the environment “can support” in a given area—to define carrying capacity, despite considerable ambiguity and uncertainty in this approach. Furthermore, the studies that we reviewed spanned several levels of ecological organization: molecules to communities and up to landscapes. To add further complexity, it is not clear whether carrying capacity was intended as a dynamic concept subject to temporal variability as it was often applied in the reviewed studies. We found that carrying capacity is most often applied to studies in conservation biology, rangeland and wildlife management, aquaculture, and fisheries biology. We explore ecosystem level responses to implications of “carrying capacity” overshoot and discuss proposed mechanisms that govern ecosystem carrying capacity. We discuss the usefulness of the concept and end with suggestions to improve carrying capacity's general application in ecosystem studies. Keywords: Carrying capacity, Conservation biology, Ecosystems, Ecosystem management, Natural resources

  14. Study on Tourist Carrying Capacity Based on Matter Element Analysis

    Institute of Scientific and Technical Information of China (English)

    LiuYunguo; FanTing; LiXin; ZhouMing; WangXianhai

    2005-01-01

    This paper proposes that it is necessary to implement the concept of tourist carrying capacity to facilitate the tourism planning, and presents a method to evaluate the carrying capacity. The method called matter element analysis can solve the uncertain and incompatible problem of the evaluated factors in assessing carrying capacity.The current state of a destination's carrying capacity can be determined by establishing the standard indexes and the matter element model. Through the evaluating of the travel industry zones of the Autonomous Prefecture of Western Hunan, the method is proved to be simple and feasible, and it is improved to be significant for the tourism planning and determination as well as the sustainable development of the regional tourism.

  15. Carrying capacity of a heterogeneous lake for migrating swans

    NARCIS (Netherlands)

    Gyimesi, A.

    2010-01-01

    One way to express the value of a natural habitat is its capacity to harbour a particular target species. In the case of migratory birds, the cumulative number of birds that can be accommodated at a site for a given period of time (‘bird-days’) became an accepted currency for this carrying capacity.

  16. [Ecological carrying capacity and Chongming Island's ecological construction].

    Science.gov (United States)

    Wang, Kaiyun; Zou, Chunjing; Kong, Zhenghong; Wang, Tianhou; Chen, Xiaoyong

    2005-12-01

    This paper overviewed the goals of Chongming Island's ecological construction and its background, analyzed the current eco-economic status and constraints of the Island, and put forward some scientific issues on its ecological construction. It was suggested that for the resources-saving and sustainable development of the Island, the researches on its ecological construction should be based on its ecological carrying capacity, fully take the regional characteristics into consideration, and refer the successful development modes at home and abroad. The carrying capacity study should ground on systemic and dynamic views, give a thorough evaluation of the Island's present carrying capacity, simulate its possible changes, and forecast its demands and risks. Operable countermeasures to promote the Island's carrying capacity should be worked out, new industry structure, population scale, and optimized distribution projects conforming to regional carrying capacity should be formulated, and effective ecological security alarming and control system should be built, with the aim of providing suggestions and strategic evidences for the decision-making of economic development and sustainable environmental resources use of the region.

  17. Concept and Connotation of Water Resources Carrying Capacity in Water Ecological Civilization Construction

    Science.gov (United States)

    Chao, Zhilong; Song, Xiaoyu; Feng, Xianghua

    2018-01-01

    Water ecological civilization construction is based on the water resources carrying capacity, guided by the sustainable development concept, adhered to the human-water harmony thoughts. This paper has comprehensive analyzed the concept and characteristics of the carrying capacity of water resources in the water ecological civilization construction, and discussed the research methods and evaluation index system of water carrying capacity in the water ecological civilization construction, finally pointed out that the problems and solutions of water carrying capacity in the water ecological civilization construction and put forward the future research prospect.

  18. Urban development control based on transportation carrying capacity

    Science.gov (United States)

    Miharja, M.; Sjafruddin, A. H.

    2017-06-01

    Severe transportation problems in Indonesian urban areas are stimulated by one fundamental factor, namely lack of awareness on transportation carrying capacity in these areas development control. Urban land use development towards more physical coverage is typically not related with the capability of transportation system to accommodate additional trips volume. Lack of clear connection between development permit with its implication on the transportation side has led to a phenomenon of exceeding transport demand over supply capacity. This paper discusses the concept of urban land use development control which will be related with transport carrying capacity. The discussion would cover both supply and demand sides of transportation. From supply side, the analysis regarding the capacity of transport system would take both existing as well as potential road network capacity could be developed. From demand side, the analysis would be through the control of a maximum floor area and public transport provision. Allowed maximum floor area for development would be at the level of generating traffic at reasonable volume. Ultimately, the objective of this paper is to introduce model to incorporate transport carrying capacity in Indonesian urban land use development control.

  19. Carrying capacity of water resources in Bandung Basin

    Science.gov (United States)

    Marganingrum, D.

    2018-02-01

    The concept of carrying capacity is widely used in various sectors as a management tool for sustainable development processes. This idea has also been applied in watershed or basin scale. Bandung Basin is the upstream of Citarum watershed known as one of the national strategic areas. This area has developed into a metropolitan area loaded with various environmental problems. Therefore, research that is related to environmental carrying capacity in this area becomes a strategic issue. However, research on environmental carrying capacity that has been done in this area is still partial either in water balance terminology, land suitability, ecological footprint, or balance of supply and demand of resources. This paper describes the application of the concept of integrated environmental carrying capacity in order to overcome the increasing complexity and dynamic environmental problems. The sector that becomes the focus of attention is the issue of water resources. The approach method to be carried out is to combine the concept of maximum balance and system dynamics. The dynamics of the proposed system is the ecological dynamics and population that cannot be separated from one another as a unity of the Bandung Basin ecosystem.

  20. Carrying capacity of Chaetoceros gracilis in Homa Lagoon and the ...

    African Journals Online (AJOL)

    The possibility for nutrient limitation to affect C. gracilis was assessed from two different ecosystems (Izmir Bay and Homa Lagoon). Our goal was to determine the growth rate of all nutrients and the maximum levels of the C. gracilis phytoplankton biomass (the maximum biomass carrying capacity) on the extent of its full ...

  1. The logistic model-generated carrying capacities for wild herbivores ...

    African Journals Online (AJOL)

    Jesse

    Under this formulation, both carrying capacity and exchange ratios are endogenously determined (Kinyua and Njoka, 2001), making it possible to empirically estimate the population growth models for Grant's gazelle, Thompson's gazelle and Zebra. (1) for i = 1,…,n-1 j = 1,…,2 and i ≠ j. Here Hit+1, measured in animal units, ...

  2. Random noise characterization on the carrying capacities of a ...

    African Journals Online (AJOL)

    The process of the survival of species dependent on a limited resource in a polluted environment which isnot a new idea can be described by the technique of a mathematical modelling. We have utilised the technique of a numerical simulation to study the impact of environmental random noise on the carrying capacities of ...

  3. Carrying capacity in a heterogeneous environment with habitat connectivity.

    Science.gov (United States)

    Zhang, Bo; Kula, Alex; Mack, Keenan M L; Zhai, Lu; Ryce, Arrix L; Ni, Wei-Ming; DeAngelis, Donald L; Van Dyken, J David

    2017-09-01

    A large body of theory predicts that populations diffusing in heterogeneous environments reach higher total size than if non-diffusing, and, paradoxically, higher size than in a corresponding homogeneous environment. However, this theory and its assumptions have not been rigorously tested. Here, we extended previous theory to include exploitable resources, proving qualitatively novel results, which we tested experimentally using spatially diffusing laboratory populations of yeast. Consistent with previous theory, we predicted and experimentally observed that spatial diffusion increased total equilibrium population abundance in heterogeneous environments, with the effect size depending on the relationship between r and K. Refuting previous theory, however, we discovered that homogeneously distributed resources support higher total carrying capacity than heterogeneously distributed resources, even with species diffusion. Our results provide rigorous experimental tests of new and old theory, demonstrating how the traditional notion of carrying capacity is ambiguous for populations diffusing in spatially heterogeneous environments. © 2017 John Wiley & Sons Ltd/CNRS.

  4. Time dependent variation of carrying capacity of prestressed precast beam

    Science.gov (United States)

    Le, Tuan D.; Konečný, Petr; Matečková, Pavlína

    2018-04-01

    The article deals with the evaluation of the precast concrete element time dependent carrying capacity. The variation of the resistance is inherited property of laboratory as well as in-situ members. Thus the specification of highest, yet possible, laboratory sample resistance is important with respect to evaluation of laboratory experiments based on the test machine loading capabilities. The ultimate capacity is evaluated through the bending moment resistance of a simply supported prestressed concrete beam. The probabilistic assessment is applied. Scatter of random variables of compressive strength of concrete and effective height of the cross section is considered. Monte Carlo simulation technique is used to investigate the performance of the cross section of the beam with changes of tendons’ positions and compressive strength of concrete.

  5. Langkawi Island, Social Aspect and the Carrying Capacity

    Directory of Open Access Journals (Sweden)

    Mohamad Diana

    2014-01-01

    Full Text Available The aptitude to convince a range of preferences has facilitated the island tourism attractions to achieve international recognition and at present KILIM Geopark is enjoyed by a prominent percentage of individuals wide-reaching. Conventional knowledge has that the island attractions are operating on the limited immobile resources, of which, have raised the scholars’ concerns over the carrying capacity issue. When analyzing carrying capacity, scholars have traditionally focused on the environmental part, hence, this working paper is motivated to convey onto the table issues relating to the social characteristics. This particular paper employed the self-administered questionnaire survey instrument which was structured to answer the two-fold objective specifically the tourists’ satisfaction level with their tourism experience and knowledge gap in relation to improving the island tourism. Targeting the tourists with minimum age of 18, this working paper collects information on the tourists’ perception towards hospitality, facilities and safety issues. In addition, this working paper comes to scrutinize different results of demographic factors as compared to what have been documented by earlier studies. Besides, it is learned that ‘safety issue’ variable plays an important role when it comes to the following factors: tourist arrival, access to facilities and tourism experience.

  6. Environmental sustainability control by water resources carrying capacity concept: application significance in Indonesia

    Science.gov (United States)

    Djuwansyah, M. R.

    2018-02-01

    This paper reviews the use of Water Resources carrying capacity concept to control environmental sustainability with the particular note for the case in Indonesia. Carrying capacity is a capability measure of an environment or an area to support human and the other lives as well as their activities in a sustainable manner. Recurrently water-related hazards and environmental problems indicate that the environments are exploited over its carrying capacity. Environmental carrying capacity (ECC) assessment includes Land and Water Carrying Capacity analysis of an area, suggested to always refer to the dimension of the related watershed as an incorporated hydrologic unit on the basis of resources availability estimation. Many countries use this measure to forecast the future sustainability of regional development based on water availability. Direct water Resource Carrying Capacity (WRCC) assessment involves population number determination together with their activities could be supported by available water, whereas indirect WRCC assessment comprises the analysis of supply-demand balance status of water. Water resource limits primarily environmental carrying capacity rather than the land resource since land capability constraints are easier. WRCC is a crucial factor known to control land and water resource utilization, particularly in a growing densely populated area. Even though capability of water resources is relatively perpetual, the utilization pattern of these resources may change by socio-economic and cultural technology level of the users, because of which WRCC should be evaluated periodically to maintain usage sustainability of water resource and environment.

  7. Examining the Resilience of Crop Production, Livestock Carrying Capacity, and Woodland Density in a Rural Zimbabwean Socio-Ecological System Using Agent-Based Models Representing Human Management Decisions

    Science.gov (United States)

    Eitzel Solera, M. V.; Neves, K.; Veski, A.; Solera, J.; Omoju, O. E.; Mawere Ndlovu, A.; Wilson, K.

    2016-12-01

    As climate change increases the pressures on arid ecosystems by changing timing and amount of rainfall, understanding the ways in which human management choices affect the resilience of these systems becomes key to their sustainability. On marginal farmland in Mazvihwa, Midlands Province, the historical carrying capacity of livestock has been consistently surprisingly high. We explore this phenomenon by building an agent-based model in NetLogo from a wealth of long-term data generated by the community-based participatory research team of The Muonde Trust, a Zimbabwean non-governmental organization. We combine the accumulated results of 35 years of indigenous and local knowledge with national datasets such as rainfall records. What factors keep the carrying capacity high? What management choices can maintain crops, livestock, and woodland at levels necessary for the community's survival? How do these choices affect long-term sustainability, and does increasing resilience at one scale reduce resilience at another scale? We use our agent-based model to explore the feedbacks between crops, livestock, and woodland and the impacts of various human choices as well as temporal and spatial ecological variation. By testing different scenarios, we disentangle the complex interactions between these components. We find that some factors out of the community's control can strongly affect the sustainability of the system through times of drought, and that supplementary feed may maintain livestock potentially at the expense of other resources. The challenges to resilience encountered by the farmers in Mazvihwa are not unique - many indigenous and rural people face drought and the legacies of colonialism, which contribute to lowered resilience to external challenges such as climate change, epidemics, and political instability. Using the agent-based model as a tool for synthesis and exploration initiates discussion about resilience-enhancing management choices for Mazvihwa's farmer-researchers.

  8. Carrying capacity of U.S. agricultural land: Ten diet scenarios

    Directory of Open Access Journals (Sweden)

    Christian J. Peters

    2016-07-01

    Full Text Available Abstract Strategies for environmental sustainability and global food security must account for dietary change. Using a biophysical simulation model we calculated human carrying capacity under ten diet scenarios. The scenarios included two reference diets based on actual consumption and eight “Healthy Diet” scenarios that complied with nutritional recommendations but varied in the level of meat content. We considered the U.S. agricultural land base and accounted for losses, processing conversions, livestock feed needs, suitability of land for crops or grazing, and land productivity. Annual per capita land requirements ranged from 0.13 to 1.08 ha person-1 year-1 across the ten diet scenarios. Carrying capacity varied from 402 to 807 million persons; 1.3 to 2.6 times the 2010 U.S. population. Carrying capacity was generally higher for scenarios with less meat and highest for the lacto-vegetarian diet. However, the carrying capacity of the vegan diet was lower than two of the healthy omnivore diet scenarios. Sensitivity analysis showed that carrying capacity estimates were highly influenced by starting assumptions about the proportion of cropland available for cultivated cropping. Population level dietary change can contribute substantially to meeting future food needs, though ongoing agricultural research and sustainable management practices are still needed to assure sufficient production levels.

  9. [Ecotourism carrying capacity of Hangzhou Xixi National Wetland Park in China].

    Science.gov (United States)

    Li, Rui; Rong, Liang

    2007-10-01

    In this paper, an integrated estimation on the ecotourism carrying capacity of Hangzhou Xixi National Wetland Park in China was made from the aspects of ecological carrying capacity, spatial carrying capacity, facility carrying capacity, management carrying capacity, and psychological carrying capacity. The results indicated that the tourism carrying capacity of the Park was 4 145 - 6 450 persons per day. The rational distance between man and bird was first adopted to determine the ecotourism carrying capacity of wetland, which provided an effective solution both to fully ensure bird safety and to appropriately develop wetland tourism. The estimation of psychological carrying capacity based on tourist satisfaction degree reflected more objectively the extent the tourist demands satisfied at the planning, construction and management of tour places. Such an integrated estimation method based on the distance between man and bird and the tourist satisfaction degree could be of practical and instructive significances in the planning and management of wetland parks.

  10. Carry

    DEFF Research Database (Denmark)

    Koijen, Ralph S.J.; Moskowitz, Tobias J.; Heje Pedersen, Lasse

    that include global equities, global bonds, currencies, commodities, US Treasuries, credit, and equity index options. This predictability underlies the strong returns to "carry trades" that go long high-carry and short low-carry securities, applied almost exclusively to currencies, but shown here...

  11. Carry

    DEFF Research Database (Denmark)

    Koijen, Ralph S.J.; Moskowitz, Tobias; Pedersen, Lasse Heje

    2018-01-01

    -sectionally and in time series for a host of different asset classes, including global equities, global bonds, commodities, US Treasuries, credit, and options. Carry is not explained by known predictors of returns from these asset classes, and it captures many of these predictors, providing a unifying framework...... for return predictability. We reject a generalized version of Uncovered Interest Parity and the Expectations Hypothesis in favor of models with varying risk premia, in which carry strategies are commonly exposed to global recession, liquidity, and volatility risks, though none fully explains carry’s premium....

  12. Introducing carrying capacity-based normalisation in LCA: framework and development of references at midpoint level

    DEFF Research Database (Denmark)

    Bjørn, Anders; Hauschild, Michael Zwicky

    2015-01-01

    carrying capacity-based normalisation references. The purpose of this article is to present a framework for normalisation against carrying capacity-based references and to develop average normalisation references (NR) for Europe and the world for all those midpoint impact categories commonly included....... A literature review was carried out to identify scientifically sound thresholds for each impact category. Carrying capacities were then calculated from these thresholds and expressed in metrics identical to midpoint indicators giving priority to those recommended by ILCD. NR was expressed as the carrying...... ozone formation and soil quality were found to exceed carrying capacities several times.The developed carrying capacity-based normalisation references offer relevant supplementary reference information to the currently applied references based on society’s background interventions by supporting...

  13. Preliminary research on quantitative methods of water resources carrying capacity based on water resources balance sheet

    Science.gov (United States)

    Wang, Yanqiu; Huang, Xiaorong; Gao, Linyun; Guo, Biying; Ma, Kai

    2018-06-01

    Water resources are not only basic natural resources, but also strategic economic resources and ecological control factors. Water resources carrying capacity constrains the sustainable development of regional economy and society. Studies of water resources carrying capacity can provide helpful information about how the socioeconomic system is both supported and restrained by the water resources system. Based on the research of different scholars, major problems in the study of water resources carrying capacity were summarized as follows: the definition of water resources carrying capacity is not yet unified; the methods of carrying capacity quantification based on the definition of inconsistency are poor in operability; the current quantitative research methods of water resources carrying capacity did not fully reflect the principles of sustainable development; it is difficult to quantify the relationship among the water resources, economic society and ecological environment. Therefore, it is necessary to develop a better quantitative evaluation method to determine the regional water resources carrying capacity. This paper proposes a new approach to quantifying water resources carrying capacity (that is, through the compilation of the water resources balance sheet) to get a grasp of the regional water resources depletion and water environmental degradation (as well as regional water resources stock assets and liabilities), figure out the squeeze of socioeconomic activities on the environment, and discuss the quantitative calculation methods and technical route of water resources carrying capacity which are able to embody the substance of sustainable development.

  14. Review on the Evaluation System of Public Safety Carrying Capacity about Small Town Community

    Institute of Scientific and Technical Information of China (English)

    Ming; SUN; Tianyu; ZHU

    2014-01-01

    Recently,small town community public safety problem has been increasingly highlighted,but its research is short on public safety carrying capacity. Through the investigation and study of community public safety carrying capacity,this paper analyzes the problem of community public safety in our country,to construct index evaluation system of public safety carrying capacity in small town community. DEA method is used to evaluate public safety carrying capacity in small town community,to provide scientific basis for the design of support and standardization theory about small town community in public safety planning.

  15. [Measuring water ecological carrying capacity with the ecosystem-service-based ecological footprint (ESEF) method: Theory, models and application].

    Science.gov (United States)

    Jiao, Wen-jun; Min, Qing-wen; Li, Wen-hua; Fuller, Anthony M

    2015-04-01

    Integrated watershed management based on aquatic ecosystems has been increasingly acknowledged. Such a change in the philosophy of water environment management requires recognizing the carrying capacity of aquatic ecosystems for human society from a more general perspective. The concept of the water ecological carrying capacity is therefore put forward, which considers both water resources and water environment, connects socio-economic development to aquatic ecosystems and provides strong support for integrated watershed management. In this paper, the authors proposed an ESEF-based measure of water ecological carrying capacity and constructed ESEF-based models of water ecological footprint and capacity, aiming to evaluate water ecological carrying capacity with footprint methods. A regional model of Taihu Lake Basin was constructed and applied to evaluate the water ecological carrying capacity in Changzhou City which located in the upper reaches of the basin. Results showed that human demand for water ecosystem services in this city had exceeded the supply capacity of local aquatic ecosystems and the significant gap between demand and supply had jeopardized the sustainability of local aquatic ecosystems. Considering aqua-product provision, water supply and pollutant absorption in an integrated way, the scale of population and economy aquatic ecosystems in Changzhou could bear only 54% of the current status.

  16. Prediction of bird-day carrying capacity on a staging site: a test of depletion models

    NARCIS (Netherlands)

    Nolet, B.A.; Gyimesi, A.; Klaassen, R.H.G.

    2006-01-01

    1. The carrying capacity of a site for migratory water birds, expressed in bird-days, can be of particular conservation value. Several attempts have been made to model this carrying capacity using ideal free distribution models such as, for instance, depletion models, in which the distribution is

  17. Rural Settlement Development and Environment Carrying Capacity Changes in Progo River Basin

    OpenAIRE

    Su Ritohardoyo; P Priyono

    2016-01-01

    Generally the broader rural settlement the heavier population pressure on agricultural land. It indicates that carrying capacity of the rural environment threatened lower. The spatial distribution of the threat in a river basin is quite important as one of the river basin management inputs. Therefore, this article aims at exposing result of research about influence rural population growth and rural settlement land changes to environment carrying capacity. This research was carried out in the ...

  18. Impact of Flexibility Options on Grid Economic Carrying Capacity of Solar and Wind: Three Case Studies

    Energy Technology Data Exchange (ETDEWEB)

    Denholm, Paul [National Renewable Energy Lab. (NREL), Golden, CO (United States); Novacheck, Joshua [National Renewable Energy Lab. (NREL), Golden, CO (United States); Jorgenson, Jennie [National Renewable Energy Lab. (NREL), Golden, CO (United States); O' Connell, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-12-01

    In this study, we attempt to quantify the benefits of various options of grid flexibility by measuring their impact on two measures: economic carrying capacity and system costs. Flexibility can increase economic carrying capacity and reduce overall system costs. In some cases, options that provide a limited increase in economic carrying capacity can provide significant operational savings, thus demonstrating the need to evaluate flexibility options using multiple metrics. The value of flexibility options varies regionally due to different generation mixes and types of renewables. The more rapid decline in PV value compared to wind makes PV more dependent on adding flexibility options, including transmission and energy storage.

  19. Quantifying spatially derived carrying capacity occupation: Framework for characterisation modelling and application to terrestrial acidification

    DEFF Research Database (Denmark)

    Bjørn, Anders; Margni, M.; Bulle, C.

    *year. This metric resembles that of the ecological footprint method and may be compared to the availability of land or water. The framework was applied to the terrestrial acidification impact category. The geochemical steady-state model PROFILE was used to quantify carrying capacities as deposition levels......The popularity of the ecological footprint method and the planetary boundaries concept shows an increasing interest among decision makers in comparing environmental impacts to carrying capacities of natural systems. Recently carrying capacity-based normalisation references were developed for impact...

  20. Temperature dependences of growth rates and carrying capacities of marine bacteria depart from metabolic theoretical predictions

    KAUST Repository

    Huete-Stauffer, Tamara Megan; Arandia-Gorostidi, Nestor; Dí az-Pé rez, Laura; Moran, Xose Anxelu G.

    2015-01-01

    Using the metabolic theory of ecology (MTE) framework, we evaluated over a whole annual cycle the monthly responses to temperature of the growth rates (μ) and carrying capacities (K) of heterotrophic bacterioplankton at a temperate coastal site. We

  1. Carrying Capacity Model Applied to Coastal Ecotourism of Baluran National Park, Indonesia

    Science.gov (United States)

    Armono, H. D.; Rosyid, D. M.; Nuzula, N. I.

    2017-07-01

    The resources of Baluran National Park have been used for marine and coastal ecotourism. The increasing number of visitors has led to the increasing of tourists and its related activities. This condition will cause the degradation of resources and the welfare of local communities. This research aims to determine the sustainability of coastal ecotourism management by calculating the effective number of tourists who can be accepted. The study uses the concept of tourism carrying capacity, consists the ecological environment, economic, social and physical carrying capacity. The results of the combined carrying capacity analysis in Baluran National Park ecotourism shows that the number of 3.288 people per day (151.248 tourists per year) is the maximum number of accepted tourists. The current number of tourist arrivals is only 241 people per day (87.990 tourists per year) which is far below the carrying capacity.

  2. Carrying capacity of Peucang Island for ecotourism management in Ujung Kulon National Park

    Science.gov (United States)

    Wiyono, K. H.; Muntasib, E. K. S. H.; Yulianda, F.

    2018-05-01

    Peucang Island is one of island in Ujung Kulon National Park (UKNP), appointed as priority area and welcome area for tourism. This research aimed to calculate the carrying capacity of Peucang Island for ecotourism development (Study sites of this research are Karang Copong jungle trail and 8 sites of Peucangs beach). This research used observation method (wildlife exploration, measure the lenght of jungle track, and measure 10 parameters of beach), literature study and and interview method to collect data. The data of jungle track analyzed use Cifuentes’s formula. The result showed that Karang Copong jungle trekking had 20,000 visitors/day for Physical Carrying Capacity (PCC), 4 838 visitors/day for Real Carrying Capacity (RCC), and 6 visitors/day for Efective Carrying Capacity (ECC). Observation of biological aspect showed that there were some damages of vegetation along the track, and the changes in animal behavior. The data of beach carrying capacity analyzed use Yulianda’s formula that measured with the suitability map approach. Based on the suitability map, two beaches were classified in suitable category, while six beaches) were classified in highly suitable category for tourism activities. All of the beaches had different number of carrying capacity, specifically there are 70 visitors/day in highly suitable beach and 27 visitors/day in suitable beach. The number of visitor nowadays still not exceed from carrying capacity number of PCC, RCC of jungle trails and carrying capacity of the beach area, but the number has exceeded from the ECC numbers.

  3. Conceptual Framework of Tourism Carrying Capacity for a Tourism City: Experiences from National Parks in the United States

    Institute of Scientific and Technical Information of China (English)

    Zheng Nasha; Zheng Xilai

    2010-01-01

    There is no universally-accepted definition of tourism carrying capacity(TCC).Numerical TCC focuses on use level and is considered as"a magic number"of the saturation point for tourism.There are several reasons why numerical tourism capacity is inadequate.Alternatively,tourism capacity can be defined in terms of limits of acceptable change,which shifts the focus from"how much use is too much"to"how much change is acceptable".This article proposes an improved conceptual framework for evaluating carrying capacity for the tourism city based on approaches used in US national parks,which consider the impact of human use on a city's economic,environmental/resource,and socio-cultural conditions.Based on the basic data of indicator values and relevant standards,the framework monitors the current indicators and predicts future indicator values; it can also be used to assess and predict TCC.

  4. Carrying capacities for nature parks as engines for sustainable regional development

    DEFF Research Database (Denmark)

    Brandt, Jesper; Holmes, Esbern

    as a departure for the comparison of the protectional conditions for the parks. Many other aspects of the nature and social carrying capacities however also constitute important conditions for the park management. Increasing emphasis on visitor experience is not only a challenge for the nature protection......Growth in the number of visitors is an upcoming problem in nature parks. Nature parks are at the same time facing increasing demand, falling public appropriations and receding focus on their conservation functions. To ensure a balancing of nature protection and economic utilization the concept...... of carrying capacity has received increasing attention among park-authorities all over the world. A comparative analysis of conditions and initiatives related to visitor/nature carrying capacities in 8 nature parks in the Baltic region has been carried out. All the parks are candidates for recognition...

  5. Measurement and assessment of carrying capacity of the environment in Ningbo, China.

    Science.gov (United States)

    Liu, R Z; Borthwick, Alistair G L

    2011-08-01

    Carrying Capacity of the Environment (CCE) provides a useful measure of the sustainable development of a region. Approaches that use integrated assessment instead of measurement can lead to misinterpretation of sustainable development because of confusion between Environmental Stress (ES) indexes and CCE indexes, and the selection of over-simple linear plus models. The present paper proposes a comprehensive measurement system for CCE which comprises models of natural resources capacity, environmental assimilative capacity, ecosystem services capacity, and society supporting capacity. The corresponding measurable indexes are designed to assess CCE using a carrying capacity surplus ratio model and a vector of surplus ratio of carrying capacity model. The former aims at direct comparison of ES and CCE based on the values of basic indexes, and the latter uses a Euclidean vector to assess CCE states. The measurement and assessment approaches are applicable to Strategic Environmental Assessment (SEA) and environmental planning and management. A case study is presented for Ningbo, China, whereby all the basic indexes of ECC are measured and the CCE states assessed for 2005 and 2010. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. Analysis of Environmental Carrying Capacity with Emergy Perspective of Jeju Island

    Directory of Open Access Journals (Sweden)

    Chanhoon Jung

    2018-05-01

    Full Text Available Jeju Island experienced an approximately 42% increase in energy consumption from 2006 to 2015 and the demand for energy consumption is expected to continue to increase. Thus, Jeju Island is planning a project entitled “Carbon Free Island by 2030” to promote sustainable development and is required to estimate the environmental carrying capacity for future energy demand changes. The purpose of this study was to calculate the emergy inherent in Jeju Island’s energy, materials, and information in 2015 using the emergy analysis method and local characteristics. In addition, this study aimed to estimate the emergy indices to evaluate the environmental carrying capacity for sustainable development in 2005, 2015, and 2030 considering the future energy demand. This study’s outputs provide the environmental carrying capacity with emergy indices, such as the percent renewable (%Renew, emergy yield ratio (EYR, environmental loading ratio (ELR, sustainability index (SI, and carrying capacity of the population (CCP for social and economic activities on Jeju Island, which are expected to be saturated. These findings show regions with heavy tourism require development strategies, including the concept of environmental carrying capacity.

  7. Modelling of carrying capacity in National Park - Fruška Gora (Serbia case study

    Directory of Open Access Journals (Sweden)

    Vujko Aleksandra

    2017-03-01

    Full Text Available Negative effects of tourism development in a destination are usually the consequence of the high concentration of tourists, accommodation facilities and the activities that are practiced in a relatively restricted area. One of the most important measures to protect the areas is to calculate the maximum number of tourists that can simultaneously reside in a region, i.e. the determination of the carrying capacity. This paper outlines a method for determining carrying capacity based on zoning of environmental resources and zoning within a region. The paper argues for a return to the idea of identifying maximum appropriate number of users. The main hypothesis of the paper is based on the statement that the development of tourism in Fruška Gora (Mountain National Park in Northern Serbia must be in accordance with the basic principles of sustainability, including the determination of carrying capacity. The main research goal was to show the opinion of local residents about the uncontrolled development of tourism, and to determine the carrying capacity in four sports and recreational zones of the mountain. The carrying capacity of the area is calculated by Lavery and Stanev formulas.

  8. The study of environmental carrying capacity for sustainable tourism in Telaga Warna Telaga Pengilon Nature Park, Dieng Plateu, Central Java

    Science.gov (United States)

    Melat Aryasa, Alexander; Nur Bambang, Azis; Muhammad, Fuad

    2017-06-01

    The increasing in quantity of the tourists visiting Telaga Warna Telaga Pengilon Nature Park, Dieng Plateau, Central Java, can cause a potential threat toward the conservation sustainability of the tourist attraction and the surrounding area. The utilization of conservation area for tourist attraction has to be carried out based on the principal of Environmental Carrying Capacity so that it will not affect the ecosystem. This study aims to determine the value of Telaga Warna Telaga Pengilon Nature Park environmental carrying capacity as a conservation area used for tourism activities. The environmental carrying capacities calculated in this study were physical carrying capacity, real carrying capacity, and effective carrying capacity. Results of this research show that the physical carrying capacity of The Telaga Warna Telaga Pengilon Nature Park was 31,302 visitors, the real capacity was 869 visitors/day and the effective carrying capacity was 579 visitors/day. Thus, the sustainable tourism development strategy is needed to manage the everlasting natural resources.

  9. Evaluation of Resources Carrying Capacity in China Based on Remote Sensing and GIS

    Science.gov (United States)

    Liu, K.; Gan, Y. H.; Zhang, T.; Luo, Z. Y.; Wang, J. J.; Lin, F. N.

    2018-04-01

    This paper accurately extracted the information of arable land, grassland (wetland), forest land, water area and construction land, based on 1 : 250000 basic geographic information data. It made model modification of comprehensive CCRR to achieve carrying capacity calculation taking resource quality into consideration. Ultimately it achieved a comprehensive assessment of CCRR status in China. The top ten cities where the status of carrying capacity of resources was overloaded were Wenzhou, Shanghai, Chengdu, Baoding, Shantou, Jieyang, Dongguan, Fuyang, Zhoukou and Handan. The cities were basically distributed in the central and southern areas with convenient transportation and more economically developed areas. Among the cities in surplus status, resources carrying capacity in Hulun Buir was the most abundant, followed by Heihe, Bayingolin Mongol Autonomous Prefecture, Qiqihar, Chifeng and Jiamusi, all of which were located in northeastern China with a small population and plentiful cultivated land.

  10. Botanical Composition, Grass Production, and Carrying Capacity of Pasture in Timor Tengah Selatan District

    Directory of Open Access Journals (Sweden)

    V. E. Se’u

    2015-12-01

    Full Text Available This study was conducted to analyze the botanical composition, grass production, carrying capacity, and potential production of nutrients in pasture located in Timor Tengah Selatan Regency. The experiment was conducted from February to July 2013, using field survey method. The botanical composition, grass production and carrying capacity on real condition were analyzed descriptively, while the grass production and carrying capacity based of cutting arrangement were analyzed by using randomized block factorial design with 3 altitude locations (Sub District of Mollo Utara with altitude of 1007 m above sea level; Sub District of Noebeba, 500 m ASL, and Sub District of Amanuban Selatan, 65 m ASL x 2 cutting intervals (1 and 2 month and 5 replications. The results showed that the grass type dominated the pasture in the Sub District of Mollo Utara, while legum type was more dominant in the pasture in the Sub Districts of Noebeba and Amanuban Selatan. The potential production of dry matter grass in Timor Tengah Selatan Regency based on real condition was 150 to 390 kg/ha/yr, this could accommodate 0.24 to 0.63 AU/ha/yr. The arrangement of cutting interval by 1 month in Mollo Utara and 2 months in Noebeba and Amanuban Selatan could increase (P<0.05 grass production and carrying capacity. The potential productions of grass nutrients were higher in Sub District of Mollo Utara, while potential production of grass dry matter was higher in Sub Districts of Noebeba and Amanuban Selatan. It was concluded that grass dry matter potential production and carrying capacity in Timor Tengah Selatan Regency were low. The arrangement of cutting interval could increase grass dry matter potential production, carrying capacity, nutrition production, and quality of nutrition.

  11. Management of marine cage aquaculture. Environmental carrying capacity method based on dry feed conversion rate.

    Science.gov (United States)

    Cai, Huiwen; Sun, Yinglan

    2007-11-01

    Marine cage aquaculture produces a large amount of waste that is released directly into the environment. To effectively manage the mariculture environment, it is important to determine the carrying capacity of an aquaculture area. In many Asian countries trash fish is dominantly used in marine cage aquaculture, which contains more water than pellet feed. The traditional nutrient loading analysis is for pellet feed not for trash fish feed. So, a more critical analysis is necessary in trash fish feed culturing areas. Corresponding to FCR (feed conversion rate), dry feed conversion rate (DFCR) was used to analyze the nutrient loadings from marine cage aquaculture where trash fish is used. Based on the hydrodynamic model and the mass transport model in Xiangshan Harbor, the relationship between the water quality and the waste discharged from cage aquaculture has been determined. The environmental carrying capacity of the aquaculture sea area was calculated by applying the models noted above. Nitrogen and phosphorus are the water quality parameters considered in this study. The simulated results show that the maximum nitrogen and phosphorus concentrations were 0.216 mg/L and 0.039 mg/L, respectively. In most of the sea area, the nutrient concentrations were higher than the water quality standard. The calculated environmental carrying capacity of nitrogen and phosphorus in Xiangshan Harbor were 1,107.37 t/yr and 134.35 t/yr, respectively. The waste generated from cage culturing in 2000 has already exceeded the environmental carrying capacity. Unconsumed feed has been identified as the most important origin of all pollutants in cage culturing systems. It suggests the importance of increasing the feed utilization and improving the feed composition on the basis of nutrient requirement. For the sustainable development of the aquaculture industry, it is an effective management measure to keep the stocking density and pollution loadings below the environmental carrying

  12. Estimating social carrying capacity through computer simulation modeling: an application to Arches National Park, Utah

    Science.gov (United States)

    Benjamin Wang; Robert E. Manning; Steven R. Lawson; William A. Valliere

    2001-01-01

    Recent research and management experience has led to several frameworks for defining and managing carrying capacity of national parks and related areas. These frameworks rely on monitoring indicator variables to ensure that standards of quality are maintained. The objective of this study was to develop a computer simulation model to estimate the relationships between...

  13. Enhanced load-carrying capacity of hairy surfaces floating on water.

    Science.gov (United States)

    Xue, Yahui; Yuan, Huijing; Su, Weidong; Shi, Yipeng; Duan, Huiling

    2014-05-08

    Water repellency of hairy surfaces depends on the geometric arrangement of these hairs and enables different applications in both nature and engineering. We investigate the mechanism and optimization of a hairy surface floating on water to obtain its maximum load-carrying capacity by the free energy and force analyses. It is demonstrated that there is an optimum cylinder spacing, as a result of the compromise between the vertical capillary force and the gravity, so that the hairy surface has both high load-carrying capacity and mechanical stability. Our analysis makes it clear that the setae on water striders' legs or some insects' wings are in such an optimized geometry. Moreover, it is shown that surface hydrophobicity can further increase the capacity of a hairy surface with thick cylinders, while the influence is negligible when the cylinders are thin.

  14. Water Quality Assessment and Determining the Carrying Capacity of Pollution Load Batang Kuranji River

    Science.gov (United States)

    Dewata, I.; Adri, Z.

    2018-04-01

    This study aims to determine the water quality and carrying capacity of pollution load Batang Kuranji River in the headwaters, middle, and downstream. This research is descriptive quantitative parameters of pH, BOD, COD, TSS, and DOES Depictions of river water quality refer to RegulationNo.82/2001, while determination of carrying capacity of pollution load river refers to the Kep Men LHNo.10/2003.The result is Kuranji Batang River water quality upstream region included in either category who meet the quality standard first class ofPP82/2001. TSS concentrations at head waters of 21 mg/L, BOD1,6 mg/L, COD7,99mg/L and DO 7,845 mg/L. While the carrying capacity of pollution load river in upstream region included in both categories namely BOD of 4,4 kg/sec, COD 273,60 kg/sec, TSS906,00kg/sec, and DO parameters of 49.20 kg/sec. Middle region (point 2, 3, and 4) water quality Batang Kuranji River has exceeded the quality standard of 82/2001 for class II and class III. Meanwhile, carrying capacity of pollution load river in area included in ugly category. The calculation is done with application Qual2Kw show that carrying capacity of pollution load river of BOD -857.3 kg/sec, COD -777.40 kg/sec, TSS +9511.5 kg/sec, and DO +69.30 kg/sec.

  15. Estimating a Global Hydrological Carrying Capacity Using GRACE Observed Water Stress

    Science.gov (United States)

    An, K.; Reager, J. T.; Famiglietti, J. S.

    2013-12-01

    Global population is expected to reach 9 billion people by the year 2050, causing increased demands for water and potential threats to human security. This study attempts to frame the overpopulation problem through a hydrological resources lens by hypothesizing that observed groundwater trends should be directly attributed to human water consumption. This study analyzes the relationships between available blue water, population, and cropland area on a global scale. Using satellite data from NASA's Gravity Recovery and Climate Experiment (GRACE) along with land surface model data from the Global Land Data Assimilation System (GLDAS), a global groundwater depletion trend is isolated, the validity of which has been verified in many regional studies. By using the inherent distributions of these relationships, we estimate the regional populations that have exceeded their local hydrological carrying capacity. Globally, these populations sum to ~3.5 billion people that are living in presently water-stressed or potentially water-scarce regions, and we estimate total cropland is exceeding a sustainable threshold by about 80 million km^2. Key study areas such as the North China Plain, northwest India, and Mexico City were qualitatively chosen for further analysis of regional water resources and policies, based on our distributions of water stress. These case studies are used to verify the groundwater level changes seen in the GRACE trend . Tfor the many populous, arid regions of the world that have already begun to experience the strains of high water demand.he many populous, arid regions of the world have already begun to experience the strains of high water demand. It will take a global cooperative effort of improving domestic and agricultural use efficiency, and summoning a political will to prioritize environmental issues to adapt to a thirstier planet. Global Groundwater Depletion Trend (Mar 2003-Dec 2011)

  16. Assessing the Social Carrying Capacity of Diving Sites in Mabul Island, Malaysia.

    Science.gov (United States)

    Zhang, Liye; Chung, ShanShan

    2015-12-01

    This study has explored social carrying capacity of an underwater environment based on divers' perceived crowding. Two dimensions were assessed, the number of divers seen and the proximity of diver. Data were obtained from a survey of 132 divers dived in Mabul Island, Malaysia during 2013-2014. Photographs depicting four levels of diver number and four levels of diver proximity in different combinations were shown to the respondents for assessing their acceptability. Between the two variables, the "number of divers" was the most influential factor for divers' perceived crowding. Divers would start to feel unacceptably crowded if 8-9 divers were visible to them at one time. Based on this, it is likely that the use level of diving sites in Mabul Island has already exceeded its social carrying capacity. Implications for future research and diving tourism management for Mabul Island are also discussed in the paper.

  17. Analysis on economic carrying capacity index of pig breeding in China

    Science.gov (United States)

    Leng, Bi-Bin; Liu, Jia-Ling; Xu, Yue-Feng

    2017-08-01

    In this paper, factor analysis method was employed to analyze and calculate the Gross Domestic Product (GDP) per capita in the last decade, the proportion of research and experiment development (R&D) expenditure equivalent to GDP, urban and rural residents’ pork consumption and explored the scale of Chinese pig breeding on economic carrying capacity index. The result showed that the growth of GDP had led to better techniques and higher field investment, and stronger support like science and technology from the government provided good conditions for large scale of pig breeding. Besides, the substantial increase of pork consumption between rural and urban residents has contributed to the pig breeding in large scale. As a result, the economic carrying capacity index in Chinese pig farming is on the rise.

  18. Assessing the Social Carrying Capacity of Diving Sites in Mabul Island, Malaysia

    Science.gov (United States)

    Zhang, Liye; Chung, ShanShan

    2015-12-01

    This study has explored social carrying capacity of an underwater environment based on divers' perceived crowding. Two dimensions were assessed, the number of divers seen and the proximity of diver. Data were obtained from a survey of 132 divers dived in Mabul Island, Malaysia during 2013-2014. Photographs depicting four levels of diver number and four levels of diver proximity in different combinations were shown to the respondents for assessing their acceptability. Between the two variables, the "number of divers" was the most influential factor for divers' perceived crowding. Divers would start to feel unacceptably crowded if 8-9 divers were visible to them at one time. Based on this, it is likely that the use level of diving sites in Mabul Island has already exceeded its social carrying capacity. Implications for future research and diving tourism management for Mabul Island are also discussed in the paper.

  19. Load Carrying Capacity of Metal Dowel Type Connections of Timber Structures

    Science.gov (United States)

    Gocál, Jozef

    2014-12-01

    This paper deals with the load-carrying capacity calculation of laterally loaded metal dowel type connections according to Eurocode 5. It is based on analytically derived, relatively complicated mathematical relationships, and thus it can be quite laborious for practical use. The aim is to propose a possible simplification of the calculation. Due to quite a great variability of fasteners' types and the connection arrangements, the attention is paid to the most commonly used nailed connections. There was performed quite an extensive parametric study focused on the calculation of load-carrying capacity of the simple shear and double shear plane nail connections, joining two or three timber parts of softwood or hardwood. Based on the study results, in conclusion there are presented simplifying recommendations for practical design.

  20. Recreation Carrying Capacity Facts and Considerations. Report 11. Surry Mountain Lake Project Area.

    Science.gov (United States)

    1980-07-01

    contributions of practical experience and knowledge , along with their assistance in arranging schedules, have made this carrying capacity research effort...This survey obtained six responses from boaters and water- skiers . 29 A~ .~ ~ ~ ~ ~ ~ ~ ~~~~~~~PEEI PAG ... ._DIAN.K+.+.+ 3+ ,.+ -,++ + _-NO FILM...User characteristics Table 17 indicates the characteristics of the boaters and water- skiers surveyed at Surry. The small sample size at

  1. Thermal carrying capacity for a thermally-sensitive species at the warmest edge of its range.

    Directory of Open Access Journals (Sweden)

    Daniel Ayllón

    Full Text Available Anthropogenic environmental change is causing unprecedented rates of population extirpation and altering the setting of range limits for many species. Significant population declines may occur however before any reduction in range is observed. Determining and modelling the factors driving population size and trends is consequently critical to predict trajectories of change and future extinction risk. We tracked during 12 years 51 populations of a cold-water fish species (brown trout Salmo trutta living along a temperature gradient at the warmest thermal edge of its range. We developed a carrying capacity model in which maximum population size is limited by physical habitat conditions and regulated through territoriality. We first tested whether population numbers were driven by carrying capacity dynamics and then targeted on establishing (1 the temperature thresholds beyond which population numbers switch from being physical habitat- to temperature-limited; and (2 the rate at which carrying capacity declines with temperature within limiting thermal ranges. Carrying capacity along with emergent density-dependent responses explained up to 76% of spatio-temporal density variability of juveniles and adults but only 50% of young-of-the-year's. By contrast, young-of-the-year trout were highly sensitive to thermal conditions, their performance declining with temperature at a higher rate than older life stages, and disruptions being triggered at lower temperature thresholds. Results suggest that limiting temperature effects were progressively stronger with increasing anthropogenic disturbance. There was however a critical threshold, matching the incipient thermal limit for survival, beyond which realized density was always below potential numbers irrespective of disturbance intensity. We additionally found a lower threshold, matching the thermal limit for feeding, beyond which even unaltered populations declined. We predict that most of our study

  2. Land Potential Productivity and Population Carrying Capacity of Yan’an City

    OpenAIRE

    Liu, Xiaoling; Zhang, Wei

    2013-01-01

    The productivity attenuation method is adopted to calculate land potential productivity of counties of Yan’an City and calculate population carrying capacity at current productivity level. Rsults shows that high photosynthetic potential productivity area and high light and temperature potential productivity area are mainly situated in the north, while high climatic potential area and high land potential productivity area are mainly concentrated in the south. From solar radiation, moisture a...

  3. On load carrying capacity of frames taking into account finite displacements

    International Nuclear Information System (INIS)

    Borkoaski, A.; Saran, M.

    1981-01-01

    An approximate method that takes into account the influence of finite displacements upon the load carrying capacity of planar unbraced frames is described. It is the iterative procedure where Quadratic Programming is applied for evaluation of subsequent configurations of the frame, whereas Linear Programming serves to find ultimate load factors for each configuration. Numerical tests show that the procedure gives practically acceptable results being at the same time much cheaper than the exact geometrically and physically non-linear incremental analysis. (orig.)

  4. The Tourism Carrying Capacity of Underwater Trails in Isabel Island National Park, Mexico

    Science.gov (United States)

    Ríos-Jara, Eduardo; Galván-Villa, Cristian Moisés; Rodríguez-Zaragoza, Fabián Alejandro; López-Uriarte, Ernesto; Muñoz-Fernández, Vicente Teófilo

    2013-08-01

    The popularity of ecotourism in the marine protected areas of Mexico has increased over the last 10 years; in particular there is a large development of a SCUBA diving industry in the Mexican Pacific including Isabel Island. Given the risks associated with human activity in the marine environments around this island, we propose two ecotourism management strategies: (1) the creation and use of underwater trails, and (2) the estimation of the specific tourism carrying capacity (TCC) for each trail. Six underwater trails were selected in sites that presented elements of biological, geological, and scenic interest, using information obtained during field observations. The methodology used to estimate the TCC was based upon the physical and biological conditions of each site, the infrastructure and equipment available, and the characteristics of the service providers and the administrators of the park. Correction factors of the TCC included elements of the quality of the visit and the threat and vulnerability of the marine environment of each trail (e.g., divers' expertise, size and distance between groups of divers, accessibility, wind, coral coverage). The TCC values ranged between 1,252 and 1,642 dives/year/trail, with a total of 8,597 dives/year for all six trails. Although these numbers are higher than the actual number of recreational visitors to the island (~1,000 dives per year), there is a need for adequate preventive management if the diving sites are to maintain their esthetic appeal and biological characteristics. Such management might be initially directed toward using only the sites and the TCC proposed here.

  5. The tourism carrying capacity of underwater trails in Isabel Island National Park, Mexico.

    Science.gov (United States)

    Ríos-Jara, Eduardo; Galván-Villa, Cristian Moisés; Rodríguez-Zaragoza, Fabián Alejandro; López-Uriarte, Ernesto; Muñoz-Fernández, Vicente Teófilo

    2013-08-01

    The popularity of ecotourism in the marine protected areas of Mexico has increased over the last 10 years; in particular there is a large development of a SCUBA diving industry in the Mexican Pacific including Isabel Island. Given the risks associated with human activity in the marine environments around this island, we propose two ecotourism management strategies: (1) the creation and use of underwater trails, and (2) the estimation of the specific tourism carrying capacity (TCC) for each trail. Six underwater trails were selected in sites that presented elements of biological, geological, and scenic interest, using information obtained during field observations. The methodology used to estimate the TCC was based upon the physical and biological conditions of each site, the infrastructure and equipment available, and the characteristics of the service providers and the administrators of the park. Correction factors of the TCC included elements of the quality of the visit and the threat and vulnerability of the marine environment of each trail (e.g., divers' expertise, size and distance between groups of divers, accessibility, wind, coral coverage). The TCC values ranged between 1,252 and 1,642 dives/year/trail, with a total of 8,597 dives/year for all six trails. Although these numbers are higher than the actual number of recreational visitors to the island (~1,000 dives per year), there is a need for adequate preventive management if the diving sites are to maintain their esthetic appeal and biological characteristics. Such management might be initially directed toward using only the sites and the TCC proposed here.

  6. Meta-analysis of carrying capacity and abundance-area relationships in marine fish species

    DEFF Research Database (Denmark)

    Mantzouni, Irene

    Knowledge on the carrying capacity and the abundance-area relationships of fish is critical to evaluate the impacts of exploitation and climate on the sustainability and also the recovery potential of the populations. Of particular interest is climate change, inducing major consequences for popul......Knowledge on the carrying capacity and the abundance-area relationships of fish is critical to evaluate the impacts of exploitation and climate on the sustainability and also the recovery potential of the populations. Of particular interest is climate change, inducing major consequences...... differences in the patterns were identified between stocks located in the upper and lower thermal range. In the latter, strong year-classes occurred mainly during warmer seasons and vice versa. For stocks located in the warmer waters, however, no significant patterns were obtained, suggesting that increased......, to incorporate the influence of ecosystem factors, mainly temperature and habitat size, on model parameters representing cod maximum reproductive rate and carrying capacity. The pattern of temperature effects on cod productivity at the species level were identified and SR model parameters were estimated...

  7. Evaluation of Water Resources Carrying Capacity in Shandong Province Based on Fuzzy Comprehensive Evaluation

    Directory of Open Access Journals (Sweden)

    Zhao Qiang

    2018-01-01

    Full Text Available Water resources carrying capacity is the maximum available water resources supporting by the social and economic development. Based on investigating and statisticing on the current situation of water resources in Shandong Province, this paper selects 13 factors including per capita water resources, water resources utilization, water supply modulus, rainfall, per capita GDP, population density, per capita water consumption, water consumption per million yuan, The water consumption of industrial output value, the agricultural output value of farmland, the irrigation rate of cultivated land, the water consumption rate of ecological environment and the forest coverage rate were used as the evaluation factors. Then,the fuzzy comprehensive evaluation model was used to analyze the water resources carrying capacity Force status evaluation. The results showed : The comprehensive evaluation results of water resources in Shandong Province were lower than 0.6 in 2001-2009 and higher than 0.6 in 2010-2015, which indicating that the water resources carrying capacity of Shandong Province has been improved.; In addition, most of the years a value of less than 0.6, individual years below 0.4, the interannual changes are relatively large, from that we can see the level of water resources is generally weak, the greater the interannual changes in Shandong Province.

  8. Ecological carrying capacity assessment of diving site: A case study of Mabul Island, Malaysia.

    Science.gov (United States)

    Zhang, Li-Ye; Chung, Shan-Shan; Qiu, Jian-Wen

    2016-12-01

    Despite considered a non-consumptive use of the marine environment, diving-related activities can cause damages to coral reefs. It is imminent to assess the maximum numbers of divers that can be accommodated by a diving site before it is subject to irreversible deterioration. This study aimed to assess the ecological carrying capacity of a diving site in Mabul Island, Malaysia. Photo-quadrat line transect method was used in the benthic survey. The ecological carrying capacity was assessed based on the relationship between the number of divers and the proportion of diver damaged hard corals in Mabul Island. The results indicated that the proportion of diver damaged hard corals occurred exponentially with increasing use. The ecological carrying capacity of Mabul Island is 15,600-16,800 divers per diving site per year at current levels of diver education and training with a quarterly threshold of 3900-4200 per site. Our calculation shows that management intervention (e.g. limiting diving) is justified at 8-14% of hard coral damage. In addition, the use of coral reef dominated diving sites should be managed according to their sensitivity to diver damage and the depth of the reefs. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Human skeletal muscle mitochondrial capacity.

    Science.gov (United States)

    Rasmussen, U F; Rasmussen, H N

    2000-04-01

    Under aerobic work, the oxygen consumption and major ATP production occur in the mitochondria and it is therefore a relevant question whether the in vivo rates can be accounted for by mitochondrial capacities measured in vitro. Mitochondria were isolated from human quadriceps muscle biopsies in yields of approximately 45%. The tissue content of total creatine, mitochondrial protein and different cytochromes was estimated. A number of activities were measured in functional assays of the mitochondria: pyruvate, ketoglutarate, glutamate and succinate dehydrogenases, palmitoyl-carnitine respiration, cytochrome oxidase, the respiratory chain and the ATP synthesis. The activities involved in carbohydrate oxidation could account for in vivo oxygen uptakes of 15-16 mmol O2 min-1 kg-1 or slightly above the value measured at maximal work rates in the knee-extensor model of Saltin and co-workers, i.e. without limitation from the cardiac output. This probably indicates that the maximal oxygen consumption of the muscle is limited by the mitochondrial capacities. The in vitro activities of fatty acid oxidation corresponded to only 39% of those of carbohydrate oxidation. The maximal rate of free energy production from aerobic metabolism of glycogen was calculated from the mitochondrial activities and estimates of the DeltaG or ATP hydrolysis and the efficiency of the actin-myosin reaction. The resultant value was 20 W kg-1 or approximately 70% of the maximal in vivo work rates of which 10-20% probably are sustained by the anaerobic ATP production. The lack of aerobic in vitro ATP synthesis might reflect termination of some critical interplay between cytoplasm and mitochondria.

  10. Analysis of Carrying Capacity of Agro-Ecosystem Coconut-Cattle in South Minahasa Regency

    Directory of Open Access Journals (Sweden)

    AHS Salendu

    2012-01-01

    Full Text Available Abstract. Coconut plantation is very dominating in South Minahasa regency seen from its production of 49,907.33 tons in 2010. The land under coconut trees can be used for food crops or forage. The waste from food crops is the source of animal feed, while the livestock’s manure is used to improve soil fertility under coconut tree. The research objective was to analyse the carrying capacity of the agro-ecosystem of coconut-cattle. Regency and district were determined purposively. A total of 86 farmers as respondents were determined based on the ownership of at least 2 cattle and cattle selling experience. Data were analyzed using effective potential of livestock development and land capability index (IDD. The result showed that the maximum potential of land resources (PMSL was 30,872.94 animal unit (AU. The capacity increase in cattle population based on the soil resources was 18,208.94 AU. The maximum potential based on farmer house holds was 127,023.00 AU. The value of land capability index was 2.14. The conclusion was South Minahasa Regency was still potential for cattle development regarding land resources or workforce potentials. Development of cattle can be integrated with the coconut to maintain and improve agro-ecosystem sustainability of coconut plantation. Keywords: cattle, carrying capacity, agro-ecosystem, coconut Animal Production 14(1:56-62, January 2012

  11. Carrying capacity an approaching procedure to the economic value of natural patrimony

    International Nuclear Information System (INIS)

    Blandon M, Luis; Ochoa S, German; Ospina N, Efren

    2001-01-01

    This study demonstrates a new alternative method for valorizing the natural heritage of various economically and environmentally important ecosystems. It was conducted in the Piedras Blancas, Antioquia watershed and considered the degree of conservation of the area and its capacity for supporting humans, by means of extractive economic activities. In this way the ecosystem itself provides a natural offer of goods and services and therefore a good approximation of its economic value

  12. Estimating carrying capacity in a newly colonized sand fly Lutzomyia serrana (Diptera: Psychodidae).

    Science.gov (United States)

    Santamaría, Erika; Munstermann, Leonard E; Ferro, Cristina

    2002-02-01

    The phlebotomine sand fly Lutzomyia serrana (Damasceno & Arouck) was mass-reared tinder conditions of varying densities in an effort to improve colony production efficiency. To do this, the experimental carrying capacity of a standard rearing chamber was determined, i.e., the optimum population size in relation to density (individuals per unit of space). Rearing chambers of 100 cm3 were populated with 1-50 L. serrana engorged females and an equal number of males. Laboratory conditions were maintained at 23-26 degrees C and 85-95% RH. The following parameters were recorded for each experimental chamber (three replicates): (1) female mortality without oviposition, (2) number of eggs oviposited and (3) number of adults emerging from the egg cohort. Female mortality began to increase substantially in the 26-female chamber, from 5.7% to 15% and finally reaching 60.2% in the 46-50 female chambers. In the chambers containing 1-20 females, egg number and realized adult progeny increased linearly to reach an asymptote. In the 20-50 female chambers, the number of eggs ranged from 420 to 699, and adult production from 306 to 432. The optimum carrying capacity for the 100-cm3 chambers was 22 +/- 2 females. Beyond this number, auto-regulation was initiated, i.e., female mortality without oviposition increased as the number of females per chamber increased. Total number of eggs and adult production was similar in all chambers containing 20-50 females. In conclusion, for optimizing production of mass reared sand flies, determination of the carrying capacity is essential to optimize use of insectary resources, to avoid loss of valuable potentially ovipositing females, and to increase overall production efficiency.

  13. Rural Settlement Development and Environment Carrying Capacity Changes in Progo River Basin

    Directory of Open Access Journals (Sweden)

    Su Ritohardoyo

    2016-12-01

    Full Text Available Generally the broader rural settlement the heavier population pressure on agricultural land. It indicates that carrying capacity of the rural environment threatened lower. The spatial distribution of the threat in a river basin is quite important as one of the river basin management inputs. Therefore, this article aims at exposing result of research about influence rural population growth and rural settlement land changes to environment carrying capacity. This research was carried out in the rural area in Progo river basin consists 56 sub districts (34 sub districts part of Jawa Tengah Province, and 22 sub districts part of Yogyakarta Special Region. The whole sub districts are such as unit analysis, and research method is based on secondary data analysis. Several data consist Districts Region in Figure 1997 and 2003 (Temanggung, Magelang, Kulon Progo, Sleman and Bantul such as secondary data analysis. Data analysis employs of frequency and cross tabulation, statistics of regression and test. Result of the research shows that population growth of the rural areas in Progo river basin are about 0.72% annum; or the household growth about 3.15% annum as long as five years (1996-2003. Spatial distribution of the population growth in the upper part of the Progo river basin is higher than in the middle and lower part of the basin. The number proportion of farmer in every sub district area in this river basin have increased from 69.95% in 1997 to 70.81% in the year of 2003. It means that work opportunities broadening are still sluggish. However, the number proportion of farmers in the upper part of the Progo river basin is lower than in the middle and lower part of the basin. The rates of settlement land areas changes (0.32 ha/annum as long as five years (1997-2003 is not as fast as the rates of agricultural land areas changes (0.47 ha/annum. Spatial land settlement areas changes in the lower (6.1 ha/annum and middle parts (2.4 ha/annum faster than

  14. Impact of Flexibility Options on Grid Economic Carrying Capacity of Solar and Wind: Three Case Studies

    Energy Technology Data Exchange (ETDEWEB)

    Denholm, Paul [National Renewable Energy Lab. (NREL), Golden, CO (United States); Novacheck, Joshua [National Renewable Energy Lab. (NREL), Golden, CO (United States); Jorgenson, Jennie [National Renewable Energy Lab. (NREL), Golden, CO (United States); O' Connell, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-12-01

    In this study, we attempt to quantify the benefits of various options of grid flexibility by measuring their impact on two measures: economic carrying capacity and system costs. Flexibility can increase ECC and reduce overall system costs. In some cases, options that provide a limited increase in ECC can provide significant operational savings, thus demonstrating the need to evaluate flexibility options using multiple metrics. The value of flexibility options varies regionally due to different generation mixes and types of renewables. The more rapid decline in PV value compared to wind makes PV more dependent on adding flexibility options, including transmission and energy storage.

  15. Review of evaluation on ecological carrying capacity: The progress and trend of methodology

    Science.gov (United States)

    Wang, S. F.; Xu, Y.; Liu, T. J.; Ye, J. M.; Pan, B. L.; Chu, C.; Peng, Z. L.

    2018-02-01

    The ecological carrying capacity (ECC) has been regarded as an important reference to indicate the level of regional sustainable development since the very beginning of twenty-first century. By a brief review of the main progress in ECC evaluation methodologies in recent five years, this paper systematically discusses the features and differences of these methods and expounds the current states and future development trend of ECC methodology. The result shows that further exploration in terms of the dynamic, comprehensive and intelligent assessment technologies needs to be provided in order to form a unified and scientific ECC methodology system and to produce a reliable basis for environmental-economic decision-makings.

  16. What is the carrying capacity for fish in the ocean? A meta analysis of population dynamics of North Atlantic cod

    DEFF Research Database (Denmark)

    Myers, R.A.; MacKenzie, Brian; Bowen, K.G.

    2001-01-01

    used empirical Bayes techniques to estimate the maximum reproductive rate and carrying capacity of each stock. In all cases, the empirical Bayes estimates were biologically reasonable, whereas a stock by stock analysis occasionally yielded nonsensical parameter estimates (e.g., infinite values). Our...... analysis showed that the carrying capacity per unit area varied by more than 20-fold among populations and that much of this variation was related to temperature. That is, the carrying capacity per square kilometre declines as temperature increases....

  17. Influence of the volume ratio of solid phase on carrying capacity of regular porous structure

    Directory of Open Access Journals (Sweden)

    Monkova Katarina

    2017-01-01

    Full Text Available Direct metal laser sintering is spread technology today. The main advantage of this method is the ability to produce parts which have a very complex geometry and which can be produced only in very complicated way by classical conventional methods. Special category of such components are parts with porous structure, which can give to the product extraordinary combination of properties. The article deals with some aspects that influence the manufacturing of regular porous structures in spite of the fact that input technological parameters at various samples were the same. The main goal of presented research has been to investigate the influence of the volume ratio of solid phase on carrying capacity of regular porous structure. Realized tests have indicated that the unit of regular porous structure with lower volume ratio is able to carry a greater load to failure than the unit with higher volume ratio.

  18. Thermal loss of life and load-carrying capacity of marine induction motors

    International Nuclear Information System (INIS)

    Gnacinski, P.

    2014-01-01

    Highlights: • The effect of voltage quality on induction motors is investigated. • Power quality significantly affects machine lifetime and load-carrying capacity. • Permissible load levels for induction motors are proposed. - Abstract: This work deals with the effect of a lowered voltage quality on the thermal loss of life and load-carrying capacity of marine induction cage machines. Results of experimental investigations and computer calculations are presented for two low power induction motors with different properties. One of them has a comparatively strongly-saturated magnetic circuit and is especially exposed to the risk of overheating under overvoltage. The other machine has a comparatively weakly-saturated magnetic circuit, and is especially sensitive to undervoltage. The induction motor lifetime expectancy is also estimated on the basis of the temperature coefficient of power quality, whose value is proportional to the windings temperature rise in induction motors especially sensitive to various power quality disturbances. The dependence of the temperature coefficient of power quality and permissible loads for induction motors supplied with voltages of lowered quality is proposed

  19. Hyperspectral Biofilm Classification Analysis for Carrying Capacity of Migratory Birds in the South Bay Salt Ponds

    Science.gov (United States)

    Hsu, Wei-Chen; Kuss, Amber Jean; Ketron, Tyler; Nguyen, Andrew; Remar, Alex Covello; Newcomer, Michelle; Fleming, Erich; Debout, Leslie; Debout, Brad; Detweiler, Angela; hide

    2011-01-01

    Tidal marshes are highly productive ecosystems that support migratory birds as roosting and over-wintering habitats on the Pacific Flyway. Microphytobenthos, or more commonly 'biofilms' contribute significantly to the primary productivity of wetland ecosystems, and provide a substantial food source for macroinvertebrates and avian communities. In this study, biofilms were characterized based on taxonomic classification, density differences, and spectral signatures. These techniques were then applied to remotely sensed images to map biofilm densities and distributions in the South Bay Salt Ponds and predict the carrying capacity of these newly restored ponds for migratory birds. The GER-1500 spectroradiometer was used to obtain in situ spectral signatures for each density-class of biofilm. The spectral variation and taxonomic classification between high, medium, and low density biofilm cover types was mapped using in-situ spectral measurements and classification of EO-1 Hyperion and Landsat TM 5 images. Biofilm samples were also collected in the field to perform laboratory analyses including chlorophyll-a, taxonomic classification, and energy content. Comparison of the spectral signatures between the three density groups shows distinct variations useful for classification. Also, analysis of chlorophyll-a concentrations show statistically significant differences between each density group, using the Tukey-Kramer test at an alpha level of 0.05. The potential carrying capacity in South Bay Salt Ponds is estimated to be 250,000 birds.

  20. Relationships between nutritional condition of adult females and relative carrying capacity for rocky mountain Elk

    Science.gov (United States)

    Piasecke, J.R.; Bender, L.C.

    2009-01-01

    Lactation can have significant costs to individual and population-level productivity because of the high energetic demands it places on dams. Because the difference in condition between lactating and dry Rocky Mountain elk (Cervus elaphus nelsoni) cows tends to disappear as nutritional quality rises, the magnitude of that difference could be used to relate condition to habitat quality or the capability of habitats to support elk. We therefore compared nutritional condition of ???2.5-yr-old lactating and dry cows from six free-ranging RockyMountain elk populations throughout the United States.Our goal was to quantify differential accrual of body fat (BF) reserves to determine whether the condition of dry and lactating cows could be used to define relevant management thresholds of habitat quality (i.e., relative carrying capacity) and consequently potential performance of elk populations. Levels of BF that lactating cows were able to accrue in autumn and the proportional difference in BF between dry and lactating cows in autumn were related (F 1-2,10???16.2, Plogistic model to predict relative proximity to ecological carrying capacity (ECC), our population-years ranged from3-97%ofECCand proportion of the population lactating (an index of calf survival) was negatively related to proportion of ECC. Results indicate that the proportional difference in accrual of BF between lactating and dry cows can provide a sensitive index to where elk populations reside relative to the quality of their range.

  1. One hundred fold increase in current carrying capacity in a carbon nanotube-copper composite

    Science.gov (United States)

    Subramaniam, Chandramouli; Yamada, Takeo; Kobashi, Kazufumi; Sekiguchi, Atsuko; Futaba, Don N.; Yumura, Motoo; Hata, Kenji

    2013-07-01

    Increased portability, versatility and ubiquity of electronics devices are a result of their progressive miniaturization, requiring current flow through narrow channels. Present-day devices operate close to the maximum current-carrying-capacity (that is, ampacity) of conductors (such as copper and gold), leading to decreased lifetime and performance, creating demand for new conductors with higher ampacity. Ampacity represents the maximum current-carrying capacity of the object that depends both on the structure and material. Here we report a carbon nanotube-copper composite exhibiting similar conductivity (2.3-4.7 × 105Scm-1) as copper (5.8 × 105Scm-1), but with a 100-times higher ampacity (6 × 108Acm-2). Vacuum experiments demonstrate that carbon nanotubes suppress the primary failure pathways in copper as observed by the increased copper diffusion activation energy (~2.0eV) in carbon nanotube-copper composite, explaining its higher ampacity. This is the only material with both high conductivity and high ampacity, making it uniquely suited for applications in microscale electronics and inverters.

  2. Characteristics and environmental carrying capacities of coastal area in Yogyakarta Special Region for aquaculture

    Science.gov (United States)

    Triyatmo, B.; Rustadi; Priyono, S. B.

    2018-03-01

    The purpose of this study were to determine characteristics and environmental carrying capacities of coastal area in Yogyakarta Special Region for aquaculture. This study was conducted in 2015 by characterizing land and water dynamics, land use, and the suitability of coastal environments for aquaculture. Evaluation on the coastal environments suitability for aquaculture ponds was based on the landforms, soil properties, water quality and land. Selection of coastal locations for aquaculture development was based on the level of suitability of coastal environment. The results showed that the coastal in Kulon Progo and Bantul Regencies were characterized by sand dune and beach ridge with sandy soil texture, while in Gunungkidul Regency was characterized by limestone hill with rocky texture. Water sources of the coastal area were the sea, river, and ground water with the salinity of 31–37, 7–11, 7–31 ppt and pH of 7.4–8.4 7.0–8.2 and 7.4–9.9, respectively. The coastal lands were used for seasonal/annual planting, ponds, fish landing sites, tourism areas and conservation areas. The coastal carrying capacity was rather suitable for aquaculture, especially in the sandy soil area. Aquaculture in that area can be done intensively for shrimp (Litopenaeus vannamei), using biocrete (biological material) or plastic sheet.

  3. Ecological Carrying Capacity of Land Use Changes in Da'an City

    Science.gov (United States)

    Wang, H.; Zhang, J.; Li, B.

    2018-04-01

    Based on GIS and RS technology, this paper analyzed the land use change in Da'an city from 1995 to 2010. land-use ecological evaluation index was constructed to evaluate the land-use ecological risk of Da 'an city dynamically, and the land-use ecological risk level map was made, and then the distribution and change of the land-use ecological carrying capacity pattern of Da'an city were analyzed qualitatively. According to the evaluation results of ecological carrying capacity, the ecological environment of Da'an city has deteriorated in fifteen years. in 1995, the poor ecological environment area is mainly distributed in the northeast area of Da'an city, and the area is small, while the area of the central and southern areas is large; In 2010, the western region also appeared environmental degradation, the northeast environment deterioration is serious, the dominant area is reduced, and a small amount of deterioration in the central and southern regions. According to the study of this paper, in the future, we should strengthen the comprehensive management of this part of the area, strengthen vegetation coverage, reduce soil erosion, ensure the effective improvement of ecological environment.

  4. Study on the carry capacity of edible jellyfish fishery in Liaodong Bay

    Science.gov (United States)

    You, Kui; Bian, Yongning; Ma, Caihua; Chi, Xupeng; Liu, Zhiqiang; Zhang, Yuyu

    2016-06-01

    Jellyfish fishing is a special type of fishery that mainly exists in some countries of East and Southeast Asia. China has the largest jellyfish fishery yield in the world with an annual harvest of around 300 thousand tons. Liaodong Bay is the most important jellyfish fishery ground in China. However, due to the high benefits of jellyfish fishery, which leads to illegal and out-of-season jellyfish fishing occurring each year in Liaodong Bay. Illegal jellyfish fishery in Liaodong Bay is a typical example of the tragedy of the commons. The key problem is that fishermen seek to an illegally initiate jellyfish fishing as early as possible. In this paper, basing on the data of edible jellyfish's biology and ecology, we mainly analyzed the history of jellyfish fishery in China, especially in Liaodong bay, and then we calculated the carry capacity of edible jellyfish in Liaodong Bay which is about 300 thousand tons one year. This number is equal to the recent annual yield of edible jellyfish in China. Furthermore, basing on the carry capacity and reasonable quotas price analysis, we set up a Jellyfish fishing quotas and deficit quotas buyback system which could be a suitable and effective solution for jellyfish fishery management and development in Liaodong Bay at the underlying roots. Although China is the first country with edible jellyfish aquaculture, the annual yield of jellyfish aquaculture is only one fifth of jellyfish fishing. So, there is a very bright developing prospect about edible jellyfish aquaculture in China.

  5. A method for prediction of extent of microbial pollution of seawater and carrying capacity of beaches

    Science.gov (United States)

    Kocasoy, Günay

    1989-07-01

    The increase in the number of tourists visiting a coast is desired by most of the countries for economic reasons. However, this increase in tourism may cause pollution of the sea. This can be only avoided by proper planning and by predicting the “carrying capacity” of the coast in terms of sea pollution. This is especially important for developing countries where part of the wastewater is discharged without any treatment into the sea. In the present study the beaches were classified according to their use — and consequently the amount of waste discharged into the sea — into four groups: (1) coasts that are used only for swimming and recreational purposes; (2) coasts used simultaneously for dwelling, swimming, and recreational purposes; (3) coasts along which only dwellings exist; and (4) natural and man-made harbors, i.e., coasts used as shelters. During the survey,40,320 observations were made between December 1985 and February 1988 to determine the effect of the number of tourists on seawater quality. The results obtained were analyzed by a multilinear regression program to obtain an empirical equation giving the extent of the sea pollution in terms of coliform concentration as a function of population density and some other environmental factors. The equations derived in this study enable the determination of the carrying capacity of a beach in terms of pollution as well as the expected degree of pollution corresponding to a given population density. Furthermore, it allows the prediction of the extra carrying capacity that can be obtained by improving the waste-disposal conditions.

  6. System dynamics model of Suzhou water resources carrying capacity and its application

    Directory of Open Access Journals (Sweden)

    Li Cheng

    2010-06-01

    Full Text Available A model of Suzhou water resources carrying capacity (WRCC was set up using the method of system dynamics (SD. In the model, three different water resources utilization programs were adopted: (1 continuity of existing water utilization, (2 water conservation/saving, and (3 water exploitation. The dynamic variation of the Suzhou WRCC was simulated with the supply-decided principle for the time period of 2001 to 2030, and the results were characterized based on socio-economic factors. The corresponding Suzhou WRCC values for several target years were calculated by the model. Based on these results, proper ways to improve the Suzhou WRCC are proposed. The model also produced an optimized plan, which can provide a scientific basis for the sustainable utilization of Suzhou water resources and for the coordinated development of the society, economy, and water resources.

  7. Meta-analysis of carrying capacity and abundance-area relationships in marine fish species

    DEFF Research Database (Denmark)

    Mantzouni, Irene

    investigated also across the north Atlantic distribution of haddock. The major motivations were first, to provide an evaluation of potential thermal effects on haddock recruitment productivity across its distribution, since the species is relatively less investigated on this regard compared to cod. In addition......Knowledge on the carrying capacity and the abundance-area relationships of fish is critical to evaluate the impacts of exploitation and climate on the sustainability and also the recovery potential of the populations. Of particular interest is climate change, inducing major consequences...... morhua), herring (Clupea harengus) and haddock (Melanogrammus aeglefinus), in order to identify the effects of temperature, habitat size and life-history on their productivity patterns. The first objective was to investigate how production and survival indices of cod recruitment (i.e. the number of new...

  8. Meta-analysis of carrying capacity and abundance-area relationships in marine fish species

    DEFF Research Database (Denmark)

    Mantzouni, Irene

    , to incorporate the influence of ecosystem factors, mainly temperature and habitat size, on model parameters representing cod maximum reproductive rate and carrying capacity. The pattern of temperature effects on cod productivity at the species level were identified and SR model parameters were estimated...... on the environmental impacts on key population parameters, which is required for an ecosystem approach to cod management, particularly under ocean-warming scenarios. The commonly used SR models, Ricker and Beverton-Holt, were extended by applying hierarchical methods, mixed-effects models and Bayesian inference...... morhua), herring (Clupea harengus) and haddock (Melanogrammus aeglefinus), in order to identify the effects of temperature, habitat size and life-history on their productivity patterns. The first objective was to investigate how production and survival indices of cod recruitment (i.e. the number of new...

  9. Load carrying capacity of RCC beams by replacing steel reinforcement bars with shape memory alloy bars

    Science.gov (United States)

    Bajoria, Kamal M.; Kaduskar, Shreya S.

    2016-04-01

    In this paper the structural behavior of reinforced concrete (RC) beams with smart rebars under two point loading system has been numerically studied, using Finite Element Method. The material used in this study is Super-elastic Shape Memory Alloys (SE SMAs) which contains nickel and titanium. In this study, different quantities of steel and SMA rebars have been used for reinforcement and the behavior of these models under two point bending loading system is studied. A comparison of load carrying capacity for the model between steel reinforced concrete beam and the beam reinforced with S.M.A and steel are performed. The results show that RC beams reinforced with combination of shape memory alloy and steel show better performance.

  10. Seasonal dynamics in wheel load-carrying capacity of a loam soil in the Swiss Plateau

    DEFF Research Database (Denmark)

    Gut, S.; Chervet, A.; Stettler, Matthias

    2015-01-01

    on in situ measurements of h, measurements of precompression stress at various h and simulations of soil stress. In this work, we concentrated on prevention of subsoil compaction. Calculations were made for different tyres (standard and low-pressure top tyres) and for soil under different tillage......Subsoil compaction is a major problem in modern agriculture caused by the intensification of agricultural production and the increase in weight of agricultural machinery. Compaction in the subsoil is highly persistent and leads to deterioration of soil functions. Wheel load-carrying capacity (WLCC......) is defined as the maximum wheel load for a specific tyre and inflation pressure that does not result in soil stress in excess of soil strength. The soil strength and hence WLCC is strongly influenced by soil matric potential (h). The aim of this study was to estimate the seasonal dynamics in WLCC based...

  11. Conditions for the management of carrying capacity in the parks of Parks&Benefits

    DEFF Research Database (Denmark)

    Brandt, Jesper; Holmes, Esbern

    2011-01-01

    Growth in the number of visitors is an upcoming problem in nature parks. Nature parks are at the same time facing increasing demand, falling public appropriations and receding focus on their conservation functions. To ensure a balancing of nature protection and economic utilization the concept...... stakeholders, balancing use and protection preferably based on scientific and/or experiential cognition. The conditions for the management of carrying capacity for the 8 nature parks in the EC Baltic Project Parks&Benefits are analysed in the report. 1. Part focus on the methodology, concentrated...... on the comparison of the common conditions related to the international nature protection obligations in the parks, primarily expressed through the management under the EU Natura2000-program. In part 2, a comparison of the 8 parks concerning extent, land use composition, population in and around the park...

  12. Evaluation of the Township Proper Carrying Capacity over Qinghai-Tibet plateau by CASA model

    Science.gov (United States)

    Wu, Chengyong; Cao, Guangchao; Xue, Huaju; Jiang, Gang; Wang, Qi; Yuan, Jie; Chen, Kelong

    2018-01-01

    The existing study of proper carrying capacity (PCC) has mostly focused on province or county administrative units, which can only macroscopically master the quantitative characteristics of PCC, but could not effectively take some animal husbandry management measures that are pertinent and operational. At town-scale, this paper used CASA model to estimate the PCC in Mongolian Autonomous County of Henan, Qinghai province, China,with serious grassland degeneration that mainly caused by overgrazing. The results showed that the PCC throughout the County was 950,417 sheep unit. For the township, the PCC of Saierlong and Duosong were the largest (247,100 sheep unit) and the smallest (82,016 sheep unit) respectively. This study will provide reference data for developing sustainable development of town-scale pasture policies and also will help to evaluate the health status of the alpine grassland ecosystem on Qinghai-Tibet plateau.

  13. Methods for determining the carrying capacity of eccentrically compressed concrete elements

    Directory of Open Access Journals (Sweden)

    Starishko Ivan Nikolaevich

    2014-04-01

    Full Text Available The author presents the results of calculations of eccentrically compressed elements in the ultimate limit state of bearing capacity, taking into account all possiblestresses in the longitudinal reinforcement from the R to the R , caused by different values of eccentricity longitudinal force. The method of calculation is based on the simultaneous solution of the equilibrium equations of the longitudinal forces and internal forces with the equilibrium equations of bending moments in the ultimate limit state of the normal sections. Simultaneous solution of these equations, as well as additional equations, reflecting the stress-strain limit state elements, leads to the solution of a cubic equation with respect to height of uncracked concrete, or with respect to the carrying capacity. According to the author it is a significant advantage over the existing methods, in which the equilibrium equations using longitudinal forces obtained one value of the height, and the equilibrium equations of bending moments - another. Theoretical studies of the author, in this article and the reasons to calculate specific examples showed that a decrease in the eccentricity of the longitudinal force in the limiting state of eccentrically compressed concrete elements height uncracked concrete height increases, the tension in the longitudinal reinforcement area gradually (not abruptly goes from a state of tension compression, and load-bearing capacity of elements it increases, which is also confirmed by the experimental results. Designed journalist calculations of eccentrically compressed elements for 4 cases of eccentric compression, instead of 2 - as set out in the regulations, fully cover the entire spectrum of possible cases of the stress-strain limit state elements that comply with the European standards for reinforced concrete, in particular Eurocode 2 (2003.

  14. A review and synthesis of recreation ecology research supporting carrying capacity and visitor use management decisionmaking

    Science.gov (United States)

    Marion, Jeff

    2016-01-01

    Resource and experiential impacts associated with visitation to wilderness and other similar backcountry settings have long been addressed by land managers under the context of “carrying capacity” decisionmaking. Determining a maximum level of allowable use, below which high-quality resource and experiential conditions would be sustained, was an early focus in the 1960s and 1970s. However, decades of recreation ecology research have shown that the severity and areal extent of visitor impact problems are influenced by an interrelated array of use-related, environmental, and managerial factors. This complexity, with similar findings from social science research, prompted scientists and managers to develop more comprehensive carrying capacity frameworks, including a new Visitor Use Management framework. These frameworks rely on a diverse array of management strategies and actions, often termed a “management toolbox,” for resolving visitor impact problems. This article reviews the most recent and relevant recreation ecology studies that have been applied in wildland settings to avoid or minimize resource impacts. The key findings and their management implications are highlighted to support the professional management of common trail, recreation site, and wildlife impact problems. These studies illustrate the need to select from a more diverse array of impact management strategies and actions based on an evaluation of problems to identify the most influential factors that can be manipulated.

  15. The scaling of population persistence with carrying capacity does not asymptote in populations of a fish experiencing extreme climate variability.

    Science.gov (United States)

    White, Richard S A; Wintle, Brendan A; McHugh, Peter A; Booker, Douglas J; McIntosh, Angus R

    2017-06-14

    Despite growing concerns regarding increasing frequency of extreme climate events and declining population sizes, the influence of environmental stochasticity on the relationship between population carrying capacity and time-to-extinction has received little empirical attention. While time-to-extinction increases exponentially with carrying capacity in constant environments, theoretical models suggest increasing environmental stochasticity causes asymptotic scaling, thus making minimum viable carrying capacity vastly uncertain in variable environments. Using empirical estimates of environmental stochasticity in fish metapopulations, we showed that increasing environmental stochasticity resulting from extreme droughts was insufficient to create asymptotic scaling of time-to-extinction with carrying capacity in local populations as predicted by theory. Local time-to-extinction increased with carrying capacity due to declining sensitivity to demographic stochasticity, and the slope of this relationship declined significantly as environmental stochasticity increased. However, recent 1 in 25 yr extreme droughts were insufficient to extirpate populations with large carrying capacity. Consequently, large populations may be more resilient to environmental stochasticity than previously thought. The lack of carrying capacity-related asymptotes in persistence under extreme climate variability reveals how small populations affected by habitat loss or overharvesting, may be disproportionately threatened by increases in extreme climate events with global warming. © 2017 The Author(s).

  16. The Analysis and Calculation Method of Urban Rail Transit Carrying Capacity Based on Express-Slow Mode

    Directory of Open Access Journals (Sweden)

    Xiaobing Ding

    2016-01-01

    Full Text Available Urban railway transport that connects suburbs and city areas is characterized by uneven temporal and spatial distribution in terms of passenger flow and underutilized carrying capacity. This paper aims to develop methodologies to measure the carrying capacity of the urban railway by introducing a concept of the express-slow mode. We first explore factors influencing the carrying capacity under the express-slow mode and the interactive relationships among these factors. Then we establish seven different scenarios to measure the carrying capacity by considering the ratio of the number of the express trains and the slow trains, the station where overtaking takes place, and the number of overtaking maneuvers. Taking Shanghai Metro Line 16 as an empirical study, the proposed methods to measure the carrying capacity under different express-slow mode are proved to be valid. This paper contributes to the literature by remodifying the traditional methods to measure the carrying capacity when different express-slow modes are applied to improve the carrying capacity of the suburban railway.

  17. Model for determining the load or carrying capacity of rivers and riverbanks for recreational use

    Directory of Open Access Journals (Sweden)

    Otero, I.

    2015-12-01

    Full Text Available The aim of this article is to determine the load or functional carrying capacity for the assimilation and recovery of rivers and riverbanks by compiling information on the environment, and to assess the potential for recreation from the environmental and scenic point of view. We set out to find the most significant basis for analysing the pressures limiting carrying capacity and constraining the demand for recreational use. This methodology will help planners and legislators in their decision-making. We also propose a methodology for assessing the recreational use of riverbanks, tested in a number of sites in the Madrid Region, in terms of the satisfaction the activity produces in the users, and the load or carrying capacity of the surrounding environment. A pilot site assessment is presented as a validation of the proposed methodology.El objetivo de este artículo es, determinar la capacidad de carga o acogida funcional, de asimilación y de recuperación de los márgenes y los cursos fluviales mediante la recopilación de información del medio; el potencial recreativo se evalúa además desde un doble punto de vista: ecológico y paisajístico. La forma en que se enfoca el problema y se presenta la metodología y los resultados puede resultar de gran utilidad para planificadores y legisladores. La metodología propuesta para valorar el uso recreativo de los márgenes fluviales, se aplica a una serie de parcelas en la Comunidad de Madrid, teniendo en cuenta la satisfacción que la actividad produce en los usuarios, y la capacidad de carga o acogida del entorno en el que se desarrollan. Como resultado, se presenta la valoración de parcelas piloto, que servirían para validar la metodología propuesta. [fr] Le but de cet article est, déterminer la capacité de charge ou réception fonctionnelle, l’assimilation et rétablissement des marges et les cours de la rivière par le biais de la collecte d’informations de l’environnement: le

  18. Measurement of the carrying capacity of benthic habitats using a metabolic-rate based index.

    Science.gov (United States)

    Edgar, G J

    1993-03-01

    Carrying capacities of grazed habitats are typically expressed as numbers or biomass of animals per unit area; however, such parameters are appropriate only when the body size of animals is constant because consumption and other metabolic-rate based parameters such as respiration and production are proportional to body mass raised by a power of ≈0.75 rather than 0 or 1. Habitat carrying levels are therefore better expressed in the form of an index of total community consumption by summing the body masses of individual animals after they have been scaled using a biomass exponent of ≈0.75. A parameter scaled in this way,P 20 , varied in a predictable manner when calculated for the mobile epifaunal assemblages associated with rope fibre habitats placed at marine and estuarine sites;P 20 showed no significant difference between 17 shallow, clear-water sites worldwide, but declined consistently when photosynthesis was reduced.P 20 also did not vary significantly when calculated for the mobile epifaunal communities associated with fourAmphibolis antarctica seagrass habitats in Australia ([Formula: see text] = 100 µg ·g -1 · day -1 ), and reached but did not significantly exceed a ceiling of ≈280 μg · g -1 · day -1 forSargassum plants. These results are consistent with the hypothesis that the production of shallow-water epifaunal communities of grazers is constrained by resource ceilings which can be quantified using metabolic-rate based indices. If this "production ceiling" hypothesis is correct then diffuse competition is generally more important than predation or environmental disturbance in restricting the growth of mobile epifaunal populations.

  19. Multiple Carrying Capacities from a management-oriented perspective to operationalize sustainable tourism in protected areas.

    Science.gov (United States)

    Salerno, Franco; Viviano, Gaetano; Manfredi, Emanuela C; Caroli, Paolo; Thakuri, Sudeep; Tartari, Gianni

    2013-10-15

    This article describes how the concept of Tourism Carrying Capacity (TCC) has shifted from a uni-dimensional approach to incorporating environmental, social and political aspects. This shift is demonstrated by a study of a large, internationally popular protected area used by trekkers, the Mt. Everest Region, where qualitative data collected from visitors was combined with environmental modeling using a participatory framework. Tourist satisfaction showed positive margins for further tourist industry expansion, but current environmental conditions limit growth and further development. Space and time dimensions were also considered. We observed that the limits on growth and further development can be manipulated, with a certain degree of flexibility, through investments and regulatory measures. We hypothesized that TCC can play an important role in the management of protected areas only if it is viewed as a systematic, strategic policy tool within a planning process rather than as a unique, intrinsic number that is not modifiable. We conclude that to translate the strategy into action using standard measures, further investigation is needed to balance the various TCC components as a part of a decision-making framework that includes the integration of different cultural approaches and policy needs. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Temperature dependences of growth rates and carrying capacities of marine bacteria depart from metabolic theoretical predictions

    KAUST Repository

    Huete-Stauffer, Tamara Megan

    2015-09-11

    Using the metabolic theory of ecology (MTE) framework, we evaluated over a whole annual cycle the monthly responses to temperature of the growth rates (μ) and carrying capacities (K) of heterotrophic bacterioplankton at a temperate coastal site. We used experimental incubations spanning 6oC with bacterial physiological groups identified by flow cytometry according to membrane integrity (live), nucleic acid content (HNA and LNA) and respiratory activity (CTC+). The temperature dependence of μat the exponential phase of growth was summarized by the activation energy (E), which was variable (-0.52 to 0.72 eV) but followed a seasonal pattern, only reaching the hypothesized value for aerobic heterotrophs of 0.65 eV during the spring bloom for the most active bacterial groups (live, HNA, CTC+). K (i.e. maximum experimental abundance) peaked at 4 × 106 cells mL-1 and generally covaried with μbut, contrary to MTE predictions, it did not decrease consistently with temperature. In the case of live cells, the responses of μand K to temperature were positively correlated and related to seasonal changes in substrate availability, indicating that the responses of bacteria to warming are far from homogeneous and poorly explained by MTE at our site. © FEMS 2015.

  1. Screening for EIA in India: enhancing effectiveness through ecological carrying capacity approach.

    Science.gov (United States)

    Rajaram, T; Das, Ashutosh

    2011-01-01

    Developing countries across the world have embraced the policy of high economic growth as a means to reduce poverty. This economic growth largely based on industrial output is fast degrading the ecosystems, jeopardizing their long term sustainability. Environmental Impact Assessment (EIA) has long been recognized as a tool which can help in protecting the ecosystems and aid sustainable development. The Screening guidelines for EIA reflect the level of commitment the nation displays towards tightening its environmental protection system. The paper analyses the screening process for EIA in India and dissects the rationale behind the exclusions and thresholds set in the screening process. The screening process in India is compared with that of the European Union with the aim of understanding the extent of deviations from a screening approach in the context of better economic development. It is found that the Indian system excludes many activities from the purview of screening itself when compared to the EU. The constraints responsible for these exclusions are discussed and the shortcomings of the current command and control system of environmental management in India are also explained. It is suggested that an ecosystem carrying capacity based management system can provide significant inputs to enhance the effectiveness of EIA process from screening to monitoring. Copyright © 2010 Elsevier Ltd. All rights reserved.

  2. Cultural carrying capacity: Organ donation advocacy, discursive framing, and social media engagement.

    Science.gov (United States)

    Bail, Christopher A

    2016-09-01

    Social media sites such as Facebook have become a powerful tool for public health outreach because they enable advocacy organizations to influence the rapidly increasing number of people who frequent these forums. Yet the very open-ness of social media sites creates fierce competition for public attention. The vast majority of social media messages provoke little or no reaction because of the sheer volume of information that confronts the typical social media user each day. In this article, I present a theory of the "cultural carrying capacity" of social media messaging campaigns. I argue that advocacy organizations inspire more endorsements, comments, and shares by social media users if they diversify the discursive content of their messages. Yet too much diversification creates large, disconnected audiences that lack the sense of shared purpose necessary to sustain an online movement. To evaluate this theory, I created a Facebook application that collects social media posts produced by forty-two organ donation advocacy organizations over 1.5 years, as well as supplemental information about the organization, its audience, and the broader social context in which they interact. Time series models provide strong evidence for my theory net of demographic characteristics of social media users, the resources and tactics of each organization, and broader external factors. I conclude by discussing the implications of these findings for public health, cultural sociology, and the nascent field of computational social science. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Assessing the air pollution carrying capacity of the northern Puget Sound region: an application of TAPAS

    Energy Technology Data Exchange (ETDEWEB)

    Wood, M.C.; Childs J.E.; Marlatt, W.E.

    1980-09-01

    The technique discussed, the Topographic Air Pollution Analysis System (TAPAS), was developed to provide valid information on the dispersion capability of the lower atmosphere in areas characterized by complex terrain features. A study was undertaken to assess the air quality (SO/sub 2/) impacts associated with energy development in the region and to determine the constraints on energy development due to regulated limitations on the amount of air quality degradation allowable in the area. The emission constraint analysis for the northern Puget Sound region indicated that total SO/sub 2/ emissions in the core area (on an annual basis) are less than half of the amount that could be tolerated in the entire core region while maintaining ambient concentrations within state and federal annual average limitations. However, comparison of source characteristics with source location and grid cell emission restrictions indicated that localized areas adjacent to the major point sources are already far in excess of the SO/sub 2/ carrying capacity. This conclusion is supported by air quality monitoring data that indicate state standard violations at several receptor locations within the study area. The annual maps of wind patterns and PI-Matrix values show areas of both good and poor dispersion characteristics under the most prevalent flow conditions.

  4. Estimating habitat carrying capacity for migrating and wintering waterfowl: Considerations, pitfalls and improvements

    Science.gov (United States)

    Williams, Christopher; Dugger, Bruce D.; Brasher, Michael G.; Coluccy, John M.; Cramer, Dane M.; Eadie, John M.; Gray, Matthew J.; Hagy, Heath M.; Livolsi, Mark; McWilliams, Scott R.; Petrie, Matthew; Soulliere, Gregory J.; Tirpak, John M.; Webb, Elisabeth B.

    2014-01-01

    Population-based habitat conservation planning for migrating and wintering waterfowl in North America is carried out by habitat Joint Venture (JV) initiatives and is based on the premise that food can limit demography (i.e. food limitation hypothesis). Consequently, planners use bioenergetic models to estimate food (energy) availability and population-level energy demands at appropriate spatial and temporal scales, and translate these values into regional habitat objectives. While simple in principle, there are both empirical and theoretical challenges associated with calculating energy supply and demand including: 1) estimating food availability, 2) estimating the energy content of specific foods, 3) extrapolating site-specific estimates of food availability to landscapes for focal species, 4) applicability of estimates from a single species to other species, 5) estimating resting metabolic rate, 6) estimating cost of daily behaviours, and 7) estimating costs of thermoregulation or tissue synthesis. Most models being used are daily ration models (DRMs) whose set of simplifying assumptions are well established and whose use is widely accepted and feasible given the empirical data available to populate such models. However, DRMs do not link habitat objectives to metrics of ultimate ecological importance such as individual body condition or survival, and largely only consider food-producing habitats. Agent-based models (ABMs) provide a possible alternative for creating more biologically realistic models under some conditions; however, ABMs require different types of empirical inputs, many of which have yet to be estimated for key North American waterfowl. Decisions about how JVs can best proceed with habitat conservation would benefit from the use of sensitivity analyses that could identify the empirical and theoretical uncertainties that have the greatest influence on efforts to estimate habitat carrying capacity. Development of ABMs at

  5. Modelling PM 10 concentrations and carrying capacity associated with woodheater emissions in Launceston, Tasmania

    Science.gov (United States)

    Luhar, Ashok K.; Galbally, Ian E.; Keywood, Melita

    Launceston is one of the Australian cities most affected by particle pollution due to the use of woodheaters in the winter months, with frequent exceedences of the national standard, the National Environment Protection Measure for Ambient Air Quality (or Air NEPM in short), of 50 micrograms per cubic metre for daily PM 10 (particulate matter with an aerodynamic diameter of 10 μm or less). The main objective of the present study was to determine the woodheater carrying capacity for Launceston—the number of woodheaters that can operate in the city without exceeding the Air NEPM. For this purpose, a prognostic meteorological and air pollution model called TAPM is used, coupled to a gridded woodheater PM 10 emissions inventory. The latter was derived using information on dwelling density, the percentage of dwellings with woodheaters, woodheater emission rates and their diurnal and seasonal variations, and the proportions of compliant/non-compliant woodheaters and open fireplaces. The model simulations are performed for the year 1998, and the concentrations are scaled for previous and subsequent years using trends in woodheater numbers and types. The modelled number of exceedences of the Air NEPM for the period 1997-2004 is in good agreement with the observations. The modelling indicates that the PM 10 Air NEPM would be met in Launceston when the total number of woodheaters is 20% of the total number of dwellings, of which 76%, 18%, 6% would be compliant woodheaters, non-compliant woodheaters and open fireplaces, respectively. With the present trends in the regional woodheater profile, this should occur in the year 2007.

  6. Changes in agriculture and abundance of snow geese affect carrying capacity of sandhill cranes in Nebraska

    Science.gov (United States)

    Pearse, A.T.; Krapu, G.L.; Brandt, D.A.; Kinzel, P.J.

    2010-01-01

    The central Platte River valley (CPRV) in Nebraska, USA, is a key spring-staging area for approximately 80 of the midcontinent population of sandhill cranes (Grus canadensis; hereafter cranes). Evidence that staging cranes acquired less lipid reserves during the 1990s compared to the late 1970s and increases in use of the CPRV by snow geese (Chen caerulescens) prompted us to investigate availability of waste corn and quantify spatial and temporal patterns of crane and waterfowl use of the region. We developed a predictive model to assess impacts of changes in availability of corn and snow goose abundance under past, present, and potential future conditions. Over a hypothetical 60-day staging period, predicted energy demand of cranes and waterfowl increased 87 between the late 1970s and 19982007, primarily because peak abundances of snow geese increased by 650,000 and cranes by 110,000. Compared to spring 1979, corn available when cranes arrived was 20 less in 1998 and 68 less in 1999; consequently, the area of cornfields required to meet crane needs increased from 14,464 ha in 1979 to 32,751 ha in 1998 and 90,559 ha in 1999. Using a pooled estimate of 88 kg/ha from springs 19981999 and 20052007, the area of cornfields needed to supply food requirements of cranes and waterfowl increased to 65,587 ha and was greatest in the eastern region of the CPRV, where an estimated 54 of cranes, 47 of Canada geese (Branta canadensis), 45 of greater white-fronted geese (Anser albifrons), and 46 of snow geese occurred during ground surveys. We estimated that a future reduction of 25 in available corn or cornfields would increase daily foraging flight distances of cranes by 2738. Crane use and ability of cranes to store lipid reserves in the CPRV could be reduced substantially if flight distance required to locate adequate corn exceeded a physiological maximum distance cranes could fly in search of food. Options to increase carrying capacity for cranes include increasing

  7. Water Environmental Carrying Capacity for Activities of Intensive Shrimp Farm in Banyuputih Sub-District, Situbondo Regency

    Directory of Open Access Journals (Sweden)

    Abdul Muqsith

    2014-09-01

    Full Text Available This research was aimed to determine the width area of an intensive shrimp farm which can be supported by water environment of Banyuputih Sub-District, Situbondo Regency according to the water assimilation capacity towards the farm’s organic waste. We used survey method and secondary data collection from other researches or result report of relevant institution. This study was using two approaches of environmental carrying capacities, i.e. (1 quantitative method on available water volume in coast (coastal water and (2 quantitative method on available Dissolved Oxygen (DO in waste receiving water body of coastal water. The result showed that the utilization of coastal area for intensive shrimp farm activities in this study area is not exceeding its carrying capacity of water yet. Based on the analysis, the water environmental carrying capacity for the farm’s organic waste is 375.637 kg TSS. Organic waste (TSS disposed from an intensive shrimp cultivation at the study area is 6.506 kg TSS.ha-1.MT-1. The width of intensive area which can be supported by Banyuputih Situbondo water environment is 58 ha. The width of available intensive pond area at study site is 113 ha, but only 39 ha is active/productive, the remaining 74 ha is inactive/unproductive area. If unproductive area will be re-operated/reactivated again, then we would recommend only 19 ha according to the water environment carrying capacity for the farm’s organic waste (58 ha. Estimation result of water environment carrying capacity for this organic waste of shrimp farm can be used as reference to determine the development or management threshold of sustainable pond area at this study site.   Keywords: environment carrying capacity, organic waste, shrimp farm

  8. Simple Evaluation of Load-Carrying Capacity of Multi-Span Folding Bridges based on Floating Supports

    Directory of Open Access Journals (Sweden)

    Jan Marszałek

    2014-12-01

    Full Text Available [b]Abstract[/b]. The papers covers simple evaluation of load-carrying capacity of multi-span folding bridges based on floating supports. Combined bridges built in this approach, could be used as a temporary crossing. The methodology of this evaluation is shown graphically on the basis of designed nomograms for two existing bridge structures i.e. MS-54 and DMS-65, mounted on rigid and floating supports. These nomograms facilitate the simple and fast determination of the impact of changing fixed support into floating support with different bridge length spans on the carrying capacity of the bridge. The paper also presents the influence of long-term use (enlarging the mounting backlash in the joints of these structures on the carrying capacity of the bridge.[b]Keywords[/b]: building, folding bridges, nomograms, assembly clearances

  9. The Application of a Phosphorus Budget Model Estimating the Carrying Capacity of Kesikköprü Dam Lake

    OpenAIRE

    PULATSÜ, Serap

    2014-01-01

    The aim of this study was to estimate the carrying capacity of Kesikköprü Dam Lake, Ankara, where cage farms for the intensive culture of rainbow trout are located. For this purpose Dillon and Rigler's phosphorus budget model was applied in a series of steps and the carrying capacity of the lake was found to be 3335 tonnes per year. This estimated value was about 10 times higher than the present production level of the lake. It seems possible to orientate the fish culture in cages in...

  10. Glove powder's carrying capacity for latex protein: analysis using the ASTM ELISA test.

    Science.gov (United States)

    Beezhold, D; Horton, K; Hickey, V; Daddona, J; Kostyal, D

    2003-01-01

    Glove donning powders carry latex proteins and disperse them into the workplace environment. We have used the ASTM D6499 ELISA to quantify the amount of latex antigen bound to and carried by glove powders. We could differentiate between a small amount of protein actually bound to the powders and a larger amount carried by the powder. Enhanced binding of a major allergen, Hev b 5, to the starch powders was demonstrated by Western blot. The D6499 ELISA is able to measure total latex antigen, soluble and powder bound, simultaneously without the need to centrifuge the samples.

  11. Parametrical Method for Determining Optimal Ship Carrying Capacity and Performance of Handling Equipment

    Directory of Open Access Journals (Sweden)

    Michalski Jan P.

    2016-04-01

    Full Text Available The paper presents a method of evaluating the optimal value of the cargo ships deadweight and the coupled optimal value of cargo handling capacity. The method may be useful at the stage of establishing the main owners requirements concerning the ship design parameters as well as for choosing a proper second hand ship for a given transportation task. The deadweight and the capacity are determined on the basis of a selected economic measure of the transport effectiveness of ship – the Required Freight Rate. The mathematical model of the problem is of a deterministic character and the simplifying assumptions are justified for ships operating in the liner trade. The assumptions are so selected that solution of the problem is obtained in analytical closed form. The presented method can be useful for application in the preliminary ship design or in the simulation of pre-investment transportation task studies.

  12. The study of carrying capacity of timber slabs with use the finite elements method

    Directory of Open Access Journals (Sweden)

    Demeshok Vitalii

    2017-01-01

    Full Text Available In the article, the results of the study of behavior of timber slab under influence of fire with the standard “time-temperature” curve are presented. The finite element method was used for it. For the calculation we constructed a grid models of timber slabs. As a result of solution of the thermal problem was obtained temperature distribution and the graphs of maximum deflection of timber slabs and its slew rate depending on the time of the test. The obtained graphs allow to obtain data on the occurrence of the limit state of loss of bearing capacity by comparing current values of displacements and velocities with the maximum allowable. Analysis of the graphs shows that the criteria limit state of loss of bearing capacity does not occur. Calculation method of evaluating the fire resistance of timber slabs was developed. For it use database about strain-stress state of this slabs in conditions of influence of the fire.

  13. Load carrying capacity of keyed joints reinforced with high strength wire rope loops

    DEFF Research Database (Denmark)

    Jørgensen, Henrik B.; Hoang, Linh Cao

    2015-01-01

    friendly shear connection can be obtained by replacing the U-bars with high strength looped wire ropes. The wire ropes have the advantage of being flexible (they have virtually no bending stiffness) which makes installation of wall elements much easier. The looped wire ropes are usually pre-installed in so......-called wire boxes which are embedded in the precast wall elements. Once the joint is grouted with mortar, the boxes will function as shear keys and the overlapping wire loops will function as transverse reinforcement that replaces the U-bars. This paper presents a rigid-plastic upper bound model to determine...... the shear capacity of wire loop connections. Tests have shown that the shear capacity of such joints – due to the relatively high tensile strength of the wire ropes - is more prone to be governed by fracture of the joint mortar in combination with yielding of the locking bar. To model this type of failure...

  14. Challenges of human resource capacity building assistance

    International Nuclear Information System (INIS)

    Noro, Naoko

    2013-01-01

    At the first Nuclear Security Summit in Washington DC in 2010, Integrated Support Center for Nuclear Nonproliferation and Nuclear Security (ISCN) of the Japan Atomic Energy Agency was established based on Japan's National Statement which expressed Japan's strong commitment to contribute to the strengthening of nuclear security in Asian region. ISCN began its activities from JFY 2011. One of the main activities of ISCN is human resource capacity building support. Since JFY 2011, ISCN has offered various nuclear security training courses, seminars and workshops and total number of the participants to the ISCN's event reached more than 700. For the past three years, ISCN has been facing variety of challenges of nuclear security human resource assistance. This paper will briefly illustrate ISCN's achievement in the past years and introduce challenges and measures of ISCN in nuclear security human resource capacity building assistance. (author)

  15. ENVIRONMENTAL CARRYING CAPACITY BASED ON LAND BALANCE FOR EVALUATION PLANNING OF SPATIAL AND REGIONAL IN SOLOK REGENCY, WEST SUMATRA

    Directory of Open Access Journals (Sweden)

    Alvan Pahuluan

    2017-05-01

    Full Text Available Act No. 26 of 2007 on Spatial Planning stated that the central and the local government should establish Spatial and Regional Planning (RTRW by considering environmental carrying capacity. The environmental carrying capacity based RTRW will ensure the balance between the use of natural resources and the prevention of negative impacts on the environment. The objective of this study was to evaluate the implementation of RTRW at Solok Regency Year 2012 - 2031 which has been established in 2013. Evaluation was conducted by calculating land carrying capacity method to determine land supply and land demand. The results showed that the land use planned as stated in the RTRW increase the land supply in supporting bio-product production to meet the needs of the population, with surplus conditions amounted to 101,005.79 hectares. The future predictions indicated that the carrying capacity of planned land could met the needs of the population bio-product until the year 2121. The evaluation of the spatial pattern plan showed that there was a need to increase the area of housing and settlements in accordance with the rate of population increase and determine the extents area for the development of the livestock sector to improve its yield.

  16. Student Responses to a Hands-On Kinesthetic Lecture Activity for Learning about the Oxygen Carrying Capacity of Blood

    Science.gov (United States)

    Breckler, Jennifer; Yu, Justin R.

    2011-01-01

    This article describes a new hands-on, or "kinesthetic," activity for use in a physiology lecture hall to help students comprehend an important concept in cardiopulmonary physiology known as oxygen carrying capacity. One impetus for designing this activity was to address the needs of students who have a preference for kinesthetic…

  17. Carrying capacity models should not use fixed prey density thresholds : a plea for using more tools of behavioural ecology

    NARCIS (Netherlands)

    van Gils, JA; Edelaar, P; Escudero, G; Piersma, T

    Earlier studies have developed models of carrying capacity to predict the number of animals a certain area can support. These models assume that resources are not renewed after consumption ('standing stock' models), and that the initial number of prey and the rate of prey consumption determine the

  18. A Study on Load Carrying Capacity of Fly Ash Based Polymer Concrete Columns Strengthened Using Double Layer GFRP Wrapping

    Directory of Open Access Journals (Sweden)

    S. Nagan

    2014-01-01

    Full Text Available This paper investigates the suitability of glass fiber reinforced polymer (GFRP sheets in strengthening of fly ash based polymer members under compression. Experimental results revealed that load carrying capacity of the confined columns increases with GFRP sheets wrapping. Altogether 18 specimens of M30 and G30 grade short columns were fabricated. The G30 specimens were prepared separately in 8 molarity and 12 molarity of sodium hydroxide concentration. Twelve specimens for low calcium fly ash based reinforced polymer concrete and six specimens of ordinary Portland cement reinforced concrete were cast. Three specimens from each molarity fly ash based reinforced polymer concrete and ordinary Portland cement reinforced concrete were wrapped with double layer of GFRP sheets. The load carrying capacity of fly ash based polymer concrete was tested and compared with control specimens. The results show increase in load carrying capacity and ductility index for all strengthened elements. The maximum increase in load carrying capacity was 68.53% and is observed in strengthened G30 specimens.

  19. A Comparative Analysis on Assessment of Land Carrying Capacity with Ecological Footprint Analysis and Index System Method.

    Directory of Open Access Journals (Sweden)

    Yao Qian

    Full Text Available Land carrying capacity (LCC explains whether the local land resources are effectively used to support economic activities and/or human population. LCC can be evaluated commonly with two approaches, namely ecological footprint analysis (EFA and the index system method (ISM. EFA is helpful to investigate the effects of different land categories whereas ISM can be used to evaluate the contributions of social, environmental, and economic factors. Here we compared the two LCC-evaluation approaches with data collected from Xiamen City, a typical region where rapid economic growth and urbanization are found in China. The results show that LCC assessments with EFA and ISM not only complement each other but also are mutually supportive. Both assessments suggest that decreases in arable land and increasingly high energy consumption have major negative effects on LCC and threaten sustainable development for Xiamen City. It is important for the local policy makers, planners and designers to reduce ecological deficits by controlling fossil energy consumption, protecting arable land and forest land from converting into other land types, and slowing down the speed of urbanization, and to promote sustainability by controlling rural-to-urban immigration, increasing hazard-free treatment rate of household garbage, and raising energy consumption per unit industrial added value. Although EFA seems more appropriate for estimating LCC for a resource-output or self-sufficient region and ISM is more suitable for a resource-input region, both approaches should be employed when perform LCC assessment in any places around the world.

  20. Multi-scale research of time and space differences about ecological footprint and ecological carrying capacity of the water resources

    Science.gov (United States)

    Li, Jiahong; Lei, Xiaohui; Fu, Qiang; Li, Tianxiao; Qiao, Yu; Chen, Lei; Liao, Weihong

    2018-03-01

    A multi-scale assessment framework for assessing and comparing the water resource sustainability based on the ecological footprint (EF) is introduced. The study aims to manage the water resource from different views in Heilongjiang Province. First of all, from the scale of each city, the water ecological carrying capacity (ECC) was calculated from 2000 to 2011, and map the spatial distribution of the recent 3 years which show that, the water ecological carrying capacity (ECC) is uneven and has a downward trend year by year. Then, from the perspective of the five secondary partition basins in Heilongjiang Province, the paper calculated the ecological carrying capacity (ECC), the ecological footprint (EF) and ecological surplus and deficit (S&D) situation of water resources from 2000 to 2011, which show that the ecological deficit situation is more prominent in Nenjiang and Suifenhe basins which are in an unsustainable development state. Finally, from the perspective of the province, the paper calculated the ecological carrying capacity (ECC), the ecological footprint (EF) and ecological S&D of water resources from 2000 to 2011 in Heilongjiang Province, which show that the ecological footprint (EF) is in the rising trend, and the correlation coefficient between the ecological carrying capacity (ECC) and the precipitation is 0.8. There are 5 years of unsustainable development state in Heilongjiang. The proposed multi-scale assessment of WEF aims to evaluate the complex relationship between water resource supply and consumption in different spatial scales and time series. It also provides more reasonable assessment result which can be used by managers and regulators.

  1. Analysis of Load-Carrying Capacity for Redundant Free-Floating Space Manipulators in Trajectory Tracking Task

    Directory of Open Access Journals (Sweden)

    Qingxuan Jia

    2014-01-01

    Full Text Available The aim of this paper is to analyze load-carrying capacity of redundant free-floating space manipulators (FFSM in trajectory tracking task. Combined with the analysis of influential factors in load-carrying process, evaluation of maximum load-carrying capacity (MLCC is described as multiconstrained nonlinear programming problem. An efficient algorithm based on repeated line search within discontinuous feasible region is presented to determine MLCC for a given trajectory of the end-effector and corresponding joint path. Then, considering the influence of MLCC caused by different initial configurations for the starting point of given trajectory, a kind of maximum payload initial configuration planning method is proposed by using PSO algorithm. Simulations are performed for a particular trajectory tracking task of the 7-DOF space manipulator, of which MLCC is evaluated quantitatively. By in-depth research of the simulation results, significant gap between the values of MLCC when using different initial configurations is analyzed, and the discontinuity of allowable load-carrying capacity is illustrated. The proposed analytical method can be taken as theoretical foundation of feasibility analysis, trajectory optimization, and optimal control of trajectory tracking task in on-orbit load-carrying operations.

  2. Environment Carrying Capacity in Developing Innovation Center of Micro Small Medium Enterprises (MSME in Kabupaten Pandeglang, Banten Province

    Directory of Open Access Journals (Sweden)

    Muhlisin

    2015-03-01

    Full Text Available The study aims to determine environmental carrying capacity strategies for developing MSMEs Innovation Center. The study research using quantitative descriptive method and instruments questionnaire given to stakeholders and government related to sheep and goats in the Juhut village, Pandeglang. Data collected was processed premises scoring and Analytical Hierarchy Process (AHP method used software of expert choice 11. The results showed that the Carrying Capacity levels of MSME was in the quite category, while aspect of facilitation as the highest proportions and the lowest was access to information. Innovation Center of MSME strategies are through the efforts increased Environment Carrying Capacity’s variables of Innovation Center of MSME are still lacking or not meet the standards through synergy efforts by Ministry of Research of Technology and Higher Education, Provincial Government. Of Banten, district/municipal government, Institute for Agricultural Technology of Banten, and universities.

  3. Calculating the Carrying Capacity of Flexural Prestressed Concrete Beams with Non-Metallic Reinforcement

    Directory of Open Access Journals (Sweden)

    Mantas Atutis

    2011-04-01

    Full Text Available The article reviews moment resistance design methods of prestressed concrete beams with fibre-reinforced polymer (FRP reinforcement. FRP tendons exhibit linear elastic response to rupture without yielding and thus failure is expected to be brittle. The structural behaviour of beams prestressed with FRP tendons is different from beams with traditional steel reinforcement. Depending on the reinforcement ratio, the flexural behaviour of the beam can be divided into several groups. The numerical results show that depending on the nature of the element failure, moment resistance calculation results are different by using reviewed methods. It was found, that the use of non-metallic reinforcement in prestressed concrete structures is effective: moment capacity is about 5% higher than that of the beams with conventional steel reinforcement.Article in Lithuanian

  4. Population growth, economic development and carrying capacity of the environment - are they compatible or in conflict?

    Directory of Open Access Journals (Sweden)

    A. E. F. Heydom

    1996-07-01

    Full Text Available In South Africa, as in many other countries, human population growth is currently reaching levels which render competition for basic human needs such as land and water, inevitable. Concurrent to population growth is the absolute necessity for economic development and job opportunities which, in turn, make tremendous demands upon the environment and natural resource base. As a consequence ever greater emphasis is placed by the news media on apparent conflicts between industrial development and social needs on the one hand, and conservation of the environment and natural resources on the other.

  5. Carrying capacity for species richness as context for conservation: a case study of North American birds

    Science.gov (United States)

    Andrew J. Hansen; Linda Bowers Phillips; Curtis H. Flather; Jim Robinson-Cox

    2011-01-01

    We evaluated the leading hypotheses on biophysical factors affecting species richness for Breeding Bird Survey routes from areas with little influence of human activities.We then derived a best model based on information theory, and used this model to extrapolate SK across North America based on the biophysical predictor variables. The predictor variables included the...

  6. Peculiarities of carrying capacity evaluations of cylindrical CFST columns with new type casing

    Directory of Open Access Journals (Sweden)

    Sinkovskaya Olena

    2017-01-01

    Full Text Available This paper deals with a cylindrical steel-concrete load-bearing unit. The feature of the unit is a steel mesh casing that is made from the expanded metal mesh. The optimal parameters of the suggested footing are determined taking into account anisotropy, i. e. different strength and rigidity in different directions that coincide and do not coincide with the direction of reinforcement. The optimal cell angle, when a casing deformation brings about maximum compression of a concrete core, is determined. Details for theoretical calculation of the bearing capacity of a cylindrical steel-concrete load-bearing unit are provided taking into consideration a complex three-dimensional structure of the suggested mesh casing. The validity of the calculations is confirmed by comparison them with the results of the Lira software package. The results of the calculations are provided in the form of the table showing the dependence of the adduced thickness of the plate on the longitudinal movement of the strip of the steel mesh casing. The dependencies to determine the adduced thickness of the mesh casing using the graphical method are provided for all the existing types of the mesh using concrete wedges or excluding them.

  7. Load carrying capacity of shear wall t-connections reinforced with high strength wire ropes

    DEFF Research Database (Denmark)

    Jørgensen, Henrik B.; Bryndom, Thor; Larsen, Michael

    2016-01-01

    -friendly solution. The wire ropes have no bending stiffness and therefore allow for an easier vertical installation of the wall elements. During the last 10 – 15 years, a number of shear tests on plane wire rope connections have been carried out. However, to the best knowledge of the authors, tests on wire rope......Traditionally, U-bar loop connections with keyed joints have been used in vertical shear connections between precast concrete wall elements. However, in the recent years, connections with looped high strength wire ropes instead of U-bar loops have proven to be a much more construction...... connections for assembly of precast elements in different planes, such as T- and L-connections, have not yet been published. This paper presents the results of a large test series recently conducted at the University of Southern Denmark to study the shear behaviour of high strength wire rope T...

  8. Spatiotemporal Distribution of β-Amyloid in Alzheimer Disease Is the Result of Heterogeneous Regional Carrying Capacities.

    Science.gov (United States)

    Whittington, Alex; Sharp, David J; Gunn, Roger N

    2018-05-01

    β-amyloid (Aβ) accumulation in the brain is 1 of 2 pathologic hallmarks of Alzheimer disease (AD), and the spatial distribution of Aβ has been studied extensively ex vivo. Methods: We applied mathematical modeling to Aβ in vivo PET imaging data to investigate competing theories of Aβ spread in AD. Results: Our results provided evidence that Aβ accumulation starts in all brain regions simultaneously and that its spatiotemporal distribution is due to heterogeneous regional carrying capacities (regional maximum possible concentration of Aβ) for the aggregated protein rather than to longer-term spreading from seed regions. Conclusion: The in vivo spatiotemporal distribution of Aβ in AD can be mathematically modeled using a logistic growth model in which the Aβ carrying capacity is heterogeneous across the brain but the exponential growth rate and time of half maximal Aβ concentration are constant. © 2018 by the Society of Nuclear Medicine and Molecular Imaging.

  9. Load Carrying Capacity of Shear Wall T-Connections Reinforced with High Strength Wire Ropes

    DEFF Research Database (Denmark)

    Jørgensen, Henrik Brøner; Bryndum, Thor; Larsen, Michael

    2017-01-01

    Traditionally, U-bar loop connections with keyed joints have been used in vertical shear connections between precast concrete wall elements. However, in the recent years, connections with looped high strength wire ropes instead of U-bar loops have proven to be a much more construction-friendly so......Traditionally, U-bar loop connections with keyed joints have been used in vertical shear connections between precast concrete wall elements. However, in the recent years, connections with looped high strength wire ropes instead of U-bar loops have proven to be a much more construction......-friendly solution. The wire ropes have no bending stiffness and therefore allow for an easier vertical installation of the wall elements. During the last 10 – 15 years, a number of shear tests on plane wire rope connections have been carried out. However, to the best knowledge of the authors, tests on wire rope...... connections for assembly of precast elements in different planes, such as T- and L-connections, have not yet been published. This paper presents the results of a large test series recently conducted at the University of Southern Denmark to study the shear behaviour of high strength wire rope T...

  10. Approximate solution of oil film load-carrying capacity of turbulent journal bearing with couple stress flow

    Science.gov (United States)

    Zhang, Yongfang; Wu, Peng; Guo, Bo; Lü, Yanjun; Liu, Fuxi; Yu, Yingtian

    2015-01-01

    The instability of the rotor dynamic system supported by oil journal bearing is encountered frequently, such as the half-speed whirl of the rotor, which is caused by oil film lubricant with nonlinearity. Currently, more attention is paid to the physical characteristics of oil film due to an oil-lubricated journal bearing being the important supporting component of the bearing-rotor systems and its nonlinear nature. In order to analyze the lubrication characteristics of journal bearings efficiently and save computational efforts, an approximate solution of nonlinear oil film forces of a finite length turbulent journal bearing with couple stress flow is proposed based on Sommerfeld and Ocvirk numbers. Reynolds equation in lubrication of a finite length turbulent journal bearing is solved based on multi-parametric principle. Load-carrying capacity of nonlinear oil film is obtained, and the results obtained by different methods are compared. The validation of the proposed method is verified, meanwhile, the relationships of load-carrying capacity versus eccentricity ratio and width-to-diameter ratio under turbulent and couple stress working conditions are analyzed. The numerical results show that both couple stress flow and eccentricity ratio have obvious influence on oil film pressure distribution, and the proposed method approximates the load-carrying capacity of turbulent journal bearings efficiently with various width-to-diameter ratios. This research proposes an approximate solution of oil film load-carrying capacity of turbulent journal bearings with different width-to-diameter ratios, which are suitable for high eccentricity ratios and heavy loads.

  11. Alveolar gas exchange and tissue oxygenation during incremental treadmill exercise, and their associations with blood O2 carrying capacity

    Directory of Open Access Journals (Sweden)

    Antti-Pekka E. Rissanen

    2012-07-01

    Full Text Available The magnitude and timing of oxygenation responses in highly active leg muscle, less active arm muscle, and cerebral tissue, have not been studied with simultaneous alveolar gas exchange measurement during incremental treadmill exercise. Nor is it known, if blood O2 carrying capacity affects the tissue-specific oxygenation responses. Thus, we investigated alveolar gas exchange and tissue (m. vastus lateralis, m. biceps brachii, cerebral cortex oxygenation during incremental treadmill exercise until volitional fatigue, and their associations with blood O2 carrying capacity in 22 healthy men. Alveolar gas exchange was measured, and near-infrared spectroscopy (NIRS was used to monitor relative concentration changes in oxy- (Δ[O2Hb], deoxy- (Δ[HHb] and total hemoglobin (Δ[tHb], and tissue saturation index (TSI. NIRS inflection points (NIP, reflecting changes in tissue-specific oxygenation, were determined and their coincidence with ventilatory thresholds (anaerobic threshold (AT, respiratory compensation point (RC; V-slope method was examined. Blood O2 carrying capacity (total hemoglobin mass (tHb-mass was determined with the CO-rebreathing method. In all tissues, NIPs coincided with AT, whereas RC was followed by NIPs. High tHb-mass associated with leg muscle deoxygenation at peak exercise (e.g., Δ[HHb] from baseline walking to peak exercise vs. tHb-mass: r = 0.64, p < 0.01, but not with arm muscle- or cerebral deoxygenation. In conclusion, regional tissue oxygenation was characterized by inflection points, and tissue oxygenation in relation to alveolar gas exchange during incremental treadmill exercise resembled previous findings made during incremental cycling. It was also found out, that O2 delivery to less active m. biceps brachii may be limited by an accelerated increase in ventilation at high running intensities. In addition, high capacity for blood O2 carrying was associated with a high level of m. vastus lateralis deoxygenation at peak

  12. The anterior-ventrolateral temporal lobe contributes to boosting visual working memory capacity for items carrying semantic information.

    Science.gov (United States)

    Chiou, Rocco; Lambon Ralph, Matthew A

    2018-04-01

    Working memory (WM) is a buffer that temporarily maintains information, be it visual or auditory, in an active state, caching its contents for online rehearsal or manipulation. How the brain enables long-term semantic knowledge to affect the WM buffer is a theoretically significant issue awaiting further investigation. In the present study, we capitalise on the knowledge about famous individuals as a 'test-case' to study how it impinges upon WM capacity for human faces and its neural substrate. Using continuous theta-burst transcranial stimulation combined with a psychophysical task probing WM storage for varying contents, we provide compelling evidence that (1) faces (regardless of familiarity) continued to accrue in the WM buffer with longer encoding time, whereas for meaningless stimuli (colour shades) there was little increment; (2) the rate of WM accrual was significantly more efficient for famous faces, compared to unknown faces; (3) the right anterior-ventrolateral temporal lobe (ATL) causally mediated this superior WM storage for famous faces. Specifically, disrupting the ATL (a region tuned to semantic knowledge including person identity) selectively hinders WM accrual for celebrity faces while leaving the accrual for unfamiliar faces intact. Further, this 'semantically-accelerated' storage is impervious to disruption of the right middle frontal gyrus and vertex, supporting the specific and causative contribution of the right ATL. Our finding advances the understanding of the neural architecture of WM, demonstrating that it depends on interaction with long-term semantic knowledge underpinned by the ATL, which causally expands the WM buffer when visual content carries semantic information. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

  13. Optimizing the Regional Industrial Structure Based on the Environmental Carrying Capacity: An Inexact Fuzzy Multi-Objective Programming Model

    Directory of Open Access Journals (Sweden)

    Wenyi Wang

    2013-12-01

    Full Text Available An inexact fuzzy multi-objective programming model (IFMOP based on the environmental carrying capacity is provided for industrial structure optimization problems. In the IFMOP model, both fuzzy linear programming (FLP and inexact linear programming (ILP methods are introduced into a multi-objective programming framework. It allows uncertainties to be directly communicated into the problem solving processing, and it can effectively reflect the complexity and uncertainty of an industrial system without impractical simplification. The two objective functions utilized in the optimization study are the maximum total output value and population size, and the constraints include water environmental capacity, water resource supply, atmospheric environmental capacity and energy supply. The model is subsequently employed in a realistic case for industrial development in the Tongzhou district, Beijing, China. The results demonstrate that the model can help to analyze whether the environmental carrying capacity of Tongzhou can meet the needs of the social economic objectives in the new town plan in the two scenarios and can assist decision makers in generating stable and balanced industrial structure patterns with consideration of the resources, energy and environmental constraints to meet the maximum social economic efficiency.

  14. Concept of perceptual carrying capacity and its use in the creation of promotional materials of tourist destination

    Directory of Open Access Journals (Sweden)

    Stanislav Mokrý

    2013-01-01

    Full Text Available The aim of this article is to introduce results of research of perceptual carrying capacity of selected area. Concept of perceptual tourist carrying capacity aims to find the number of visitors of tourist destination that the visitor is willing to accept before deciding to prefer travelling to another tourist destination. In order to determine the perceptual carrying capacity the visual method is used. The photos of destination that depict different level of visitors are presented to respondents when using this method. The primary research method used in this investigation was the questionnaire survey which was conducted via online questionnaires. The questionnaire used computer-generated images that depict different number of visitors of the particular destination – nature trail around the church of St. John of Nepomuk in Žďár nad Sázavou (UNESCO monument. The questionnaire also contained samples of photos taken from photo gallery section of CzechTourism agency (photo.czechtourism.com, which were used to determine the preferred destination for recreation. The survey was conducted within the period from May 2012 to July 2012 on a sample of 736 respondents from The Czech Republic.

  15. The Assessment of Carrying Capacity – A Crucial T ool for M anaging T ourism E ffects in T ourist D estinations

    Directory of Open Access Journals (Sweden)

    Dobrica Jovicic

    2008-01-01

    Full Text Available It is beyond doubt that the theory of tourism lags behind tourism practice. In order to decrease such a gap, theoretical work in tourism has been intensified over the last two decades. The key problems of research include: analysis, interpretation, and management of heterogeneous effects of tourism development, permeating different aspects of human life and work – from the global to the local level. This paper discusses the role, significance, and the ways of estimation and interpretation of the concept of carrying capacity within the conceptual framework of contemporary tourism. The paper also analyses various attitudes and conflicting opinions as to this, still much debated, concept. In the end, some crucial concluding remarks are presented that could alleviate the application of the concept of carrying capacity in practice, assuming its important role in planning tourist destination development and management of various tourism impacts.

  16. Conservation endocrinology: A noninvasive tool to understand relationships between carnivore colonization and ecological carrying capacity

    Science.gov (United States)

    Berger, J.; Testa, J.W.; Roffe, T.; Monfort, S.L.

    1999-01-01

    Reproductive technology, especially the diagnosis of pregnancy by radioimmunoassay of fecal steroid metabolites, is an important component of captive propagation, but its role in our understanding of ecological interactions and in situ biological restoration has been more limited. Where large herbivores have been 'released' from predation by the extirpation of carnivores, controversy often exists about possible detrimental effects at the ecosystem level A related concern is that the reestablishment of large carnivores may decrease the availability of prey populations for human subsistence. We suggest that pregnancy assays can be a valuable tool to help distinguish between the roles of predation versus food-imposed limitations on population size and their effects on juvenile recruitment in wild species. We explored this issue through analyses of fecal progestagen concentration (FPC) levels to document pregnancy in moose (Alces alces) in the southern Greater Yellowstone Ecosystem, a site where wolves (Canis lupus) and grizzly bears (Ursus arctos) are recolonizing former habitats after an absence of more than 60 years. Pregnancy was clearly discernible (mean FPC for pregnant and nonpregnant females, respectively: 10.60 vs. 2.57 ??g/g; p endocrinology can be applied to issues involving reproductive events within an ecological context. They also affirm that noninvasive and generally inexpensive endocrinological procedures will be applicable to understanding interactions between recolonizing predators and prey, an issue that will continue to arise because of global restoration efforts, and to the study of rare ungulates in remote systems where data on reproductive events are difficult to obtain.

  17. Price and prospects for oil: carrying the burden of excess capacity in the 1980s and beyond

    Energy Technology Data Exchange (ETDEWEB)

    Goldstein, W

    1985-12-01

    The continuing decline in world oil prices will not be halted in the short term, and prospects for the long run are not encouraging. There is a problem of unprecedented gravity in the surplus capacity of the oil industry. A glut of 10 million bbl/day of crude oil remains unsold, the cohesion of the OPEC cartel is becoming more strained, and a sizeable proportion of refinery plant has been taken off-stream. The basic difficulty is that high interest rates have curbed international capital formation and depressed demand. Upward pressures on the US dollar have been created by the deficit on US domestic and external accounts, and have retarded the recovery of the global economy. Today, the cost of money exceeds the factor price of oil, and the market is highly unstable. The devastating costs of carrying surplus capacity are likely to survive through the 1980s. 6 figures.

  18. Study on tourist carrying capacity of sustainable tourism---by taking Qingliang Mountain in the south-eastern china as an example

    Science.gov (United States)

    Jin, Shenglang; Liu, Ting

    2017-05-01

    This paper analyzed measurable problems of tourist carrying capacity and then studied the corresponding measures by adopting theories and methods of environment sciences. Tourist carrying capacity of Qingliang Mountain was studied by analyzing the system of tourism environment capacity. The results showed that the level of service was 9726, which was smaller than the ecological carrying capacity of 12894. The facilities and spatial capacity were identified as key factors to limit the tourist carrying capacity in Qingliang Mountain. Some advices and measures including newly built and rebuilt basic facilities, construction of management methods for slack and peak tourist season respectively, construction of new sight spot and touring path were discussed to improve the tourist carrying capacity of Qingliang Mountain and solve the problems of overloading of tourists in the peak season.

  19. Isotopic investigation of contemporary and historic changes in penguin trophic niches and carrying capacity of the southern Indian ocean.

    Science.gov (United States)

    Jaeger, Audrey; Cherel, Yves

    2011-02-02

    A temperature-defined regime shift occurred in the 1970s in the southern Indian Ocean, with simultaneous severe decreases in many predator populations. We tested a possible biological link between the regime shift and predator declines by measuring historic and contemporary feather isotopic signatures of seven penguin species with contrasted foraging strategies and inhabiting a large latitudinal range. We first showed that contemporary penguin isotopic variations and chlorophyll a concentration were positively correlated, suggesting the usefulness of predator δ¹³C values to track temporal changes in the ecosystem carrying capacity and its associated coupling to consumers. Having controlled for the Suess effect and for increase CO₂ in seawater, δ¹³C values of Antarctic penguins and of king penguins did not change over time, while δ¹³C of other subantarctic and subtropical species were lower in the 1970s. The data therefore suggest a decrease in ecosystem carrying capacity of the southern Indian Ocean during the temperature regime-shift in subtropical and subantarctic waters but not in the vicinity of the Polar Front and in southward high-Antarctic waters. The resulting lower secondary productivity could be the main driving force explaining the decline of subtropical and subantarctic (but not Antarctic) penguins that occurred in the 1970s. Feather δ¹⁵N values did not show a consistent temporal trend among species, suggesting no major change in penguins' diet. This study highlights the usefulness of developing long-term tissue sampling and data bases on isotopic signature of key marine organisms to track potential changes in their isotopic niches and in the carrying capacity of the environment.

  20. Isotopic investigation of contemporary and historic changes in penguin trophic niches and carrying capacity of the southern Indian ocean.

    Directory of Open Access Journals (Sweden)

    Audrey Jaeger

    Full Text Available A temperature-defined regime shift occurred in the 1970s in the southern Indian Ocean, with simultaneous severe decreases in many predator populations. We tested a possible biological link between the regime shift and predator declines by measuring historic and contemporary feather isotopic signatures of seven penguin species with contrasted foraging strategies and inhabiting a large latitudinal range. We first showed that contemporary penguin isotopic variations and chlorophyll a concentration were positively correlated, suggesting the usefulness of predator δ¹³C values to track temporal changes in the ecosystem carrying capacity and its associated coupling to consumers. Having controlled for the Suess effect and for increase CO₂ in seawater, δ¹³C values of Antarctic penguins and of king penguins did not change over time, while δ¹³C of other subantarctic and subtropical species were lower in the 1970s. The data therefore suggest a decrease in ecosystem carrying capacity of the southern Indian Ocean during the temperature regime-shift in subtropical and subantarctic waters but not in the vicinity of the Polar Front and in southward high-Antarctic waters. The resulting lower secondary productivity could be the main driving force explaining the decline of subtropical and subantarctic (but not Antarctic penguins that occurred in the 1970s. Feather δ¹⁵N values did not show a consistent temporal trend among species, suggesting no major change in penguins' diet. This study highlights the usefulness of developing long-term tissue sampling and data bases on isotopic signature of key marine organisms to track potential changes in their isotopic niches and in the carrying capacity of the environment.

  1. Influence of time-dependent elastic-plastic material behaviour on the load-carrying capacity of shells of revolution

    International Nuclear Information System (INIS)

    Schnabel, F.

    1987-01-01

    The present report deals with the influence of time-dependent material behavior on the load-carrying capacity of thin-walled shells of revolution. In the first part various creep-hardening hypotheses as well as the spatial and temporal discretization procedures employed are described. The adaptation of a well-tested finite element method based on ring elements to the treatment of creep problems and several time-integration procedures, in particular the iterative treatment of the coupling between creep and elastic-plastic strains as well as the important aspect of time-step-control are discussed in detail. In the second part several typical shell configurations are analyzed and a comparison with available theoretical and experimental results is made. Finally, the time-dependent load-carrying behavior of torispherical pressure vessel ends subjected to internal and external pressure is investigated and design aids for the determination of creep collapse times are proposed. (orig.) [de

  2. Graphene-on-diamond devices with increased current-carrying capacity: carbon sp2-on-sp3 technology.

    Science.gov (United States)

    Yu, Jie; Liu, Guanxiong; Sumant, Anirudha V; Goyal, Vivek; Balandin, Alexander A

    2012-03-14

    Graphene demonstrated potential for practical applications owing to its excellent electronic and thermal properties. Typical graphene field-effect transistors and interconnects built on conventional SiO(2)/Si substrates reveal the breakdown current density on the order of 1 μA/nm(2) (i.e., 10(8) A/cm(2)), which is ~100× larger than the fundamental limit for the metals but still smaller than the maximum achieved in carbon nanotubes. We show that by replacing SiO(2) with synthetic diamond, one can substantially increase the current-carrying capacity of graphene to as high as ~18 μA/nm(2) even at ambient conditions. Our results indicate that graphene's current-induced breakdown is thermally activated. We also found that the current carrying capacity of graphene can be improved not only on the single-crystal diamond substrates but also on an inexpensive ultrananocrystalline diamond, which can be produced in a process compatible with a conventional Si technology. The latter was attributed to the decreased thermal resistance of the ultrananocrystalline diamond layer at elevated temperatures. The obtained results are important for graphene's applications in high-frequency transistors, interconnects, and transparent electrodes and can lead to the new planar sp(2)-on-sp(3) carbon-on-carbon technology. © 2012 American Chemical Society

  3. Assessing Potential Habitat and Carrying Capacity for Reintroduction of Plains Bison (Bison bison bison in Banff National Park.

    Directory of Open Access Journals (Sweden)

    Robin Steenweg

    Full Text Available Interest in bison (Bison bison, B. bonasus conservation and restoration continues to grow globally. In Canada, plains bison (B. b. bison are threatened, occupying less than 0.5% of their former range. The largest threat to their recovery is the lack of habitat in which they are considered compatible with current land uses. Fences and direct management make range expansion by most bison impossible. Reintroduction of bison into previously occupied areas that remain suitable, therefore, is critical for bison recovery in North America. Banff National Park is recognized as historical range of plains bison and has been identified as a potential site for reintroduction of a wild population. To evaluate habitat quality and assess if there is sufficient habitat for a breeding population, we developed a Habitat Suitability Index (HSI model for the proposed reintroduction and surrounding areas in Banff National Park (Banff. We then synthesize previous studies on habitat relationships, forage availability, bison energetics and snowfall scenarios to estimate nutritional carrying capacity. Considering constraints on nutritional carrying capacity, the most realistic scenario that we evaluated resulted in an estimated maximum bison density of 0.48 bison/km2. This corresponds to sufficient habitat to support at least 600 to 1000 plains bison, which could be one of the largest 10 plains bison populations in North America. Within Banff, there is spatial variation in predicted bison habitat suitability and population size that suggests one potential reintroduction site as the most likely to be successful from a habitat perspective. The successful reintroduction of bison into Banff would represent a significant global step towards conserving this iconic species, and our approach provides a useful template for evaluating potential habitat for other endangered species reintroductions into their former range.

  4. Effects of tidal cycles on shorebird distribution and foraging behaviour in a coastal tropical wetland: Insights for carrying capacity assessment

    Science.gov (United States)

    Fonseca, Juanita; Basso, Enzo; Serrano, David; Navedo, Juan G.

    2017-11-01

    Wetland loss has driven negative effects on biodiversity by a reduction in potential available habitats, directly impacting wetland-dependent species such as migratory shorebirds. At coastal areas where tidal cycles can restrict food access, the degree to which density of foraging birds is mediated by conspecific abundance or by the available areas is crucial to understanding patterns of bird distribution and wetland carrying capacity. We used the bathymetry of two sectors modeled with two numerical matrices to determine the availability of intertidal foraging areas in relation to tidal level (spring and neap tides), and this information was used to estimate shorebird density and foraging activity throughout the low-tide cycle in a tropical coastal lagoon in northwestern Mexico. Relative to spring tides, an 80% reduction in available foraging areas occurred during neap tides. Overall shorebird abundance was significantly reduced during neap tide periods, with differences between species. Densities of shorebirds increased during neap tides, particularly in one sector, and remained similar throughout the low-tide period (i.e. 4 h) either during spring or neap tides. Time spent foraging was consistently lower during neap-tides relative to spring-tides, especially for Long-billed curlew (44% reduction), Willet (37% reduction) and Black-necked stilt (29% reduction). These decreases in foraging activity when available habitats became reduced can hamper the opportunities of migratory shorebirds to reach their daily energy requirements to survive during the non-breeding season. This study shows that when intertidal habitats are severely reduced an important fraction of shorebird populations would probably be forced to find alternative areas to forage or increase foraging time during the night. Serving an essential function as top-predators, these results can have important implications on carrying capacity assessment for shorebirds at coastal wetlands.

  5. Combating (Child Human Trafficking: Building Capacity

    Directory of Open Access Journals (Sweden)

    John Winterdyk

    2018-03-01

    Full Text Available The presentation/paper focuses on the challenges and necessity of building capacity at local, national, and international levels with a focus of how to more effectively combat trafficking in human beings (THB. Insight from several of initiatives are shared with the aim of illustrating how to capitalize on the vast number of opportunities that already exist at these levels and how they might be coordinated to enable collaborative work in an informed and dynamic manner to combat human trafficking. Information from several recent research projects that focus on some of these same issues is also incorporated into this paper. El artículo se centra en los desafíos de la lucha contra el tráfico de personas y en la necesidad de aumentar la capacidad para ser más eficaces en ese sentido, local, nacional e internacionalmente. Nos hacemos eco de la visión de varias iniciativas, con el fin de ilustrar cómo capitalizar el gran número de oportunidades que ya existen en los ámbitos citados, y la forma en que se podrían coordinar para posibilitar la colaboración de una manera informada y dinámica para combatir la trata de personas. También se incluye en el artículo información emanada de diversos proyectos recientes de investigación sobre el tema que nos ocupa. DOWNLOAD THIS PAPER FROM SSRN: http://ssrn.com/abstract=3086067

  6. Grid Integration and the Carrying Capacity of the U.S. Grid to Incorporate Variable Renewable Energy

    Energy Technology Data Exchange (ETDEWEB)

    Cochran, Jaquelin [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Denholm, Paul [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Speer, Bethany [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Miller, Mackay [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2015-04-23

    In the United States and elsewhere, renewable energy (RE) generation supplies an increasingly large percentage of annual demand, including nine U.S. states where wind comprised over 10% of in-state generation in 2013. This white paper summarizes the challenges to integrating increasing amounts of variable RE, identifies emerging practices in power system planning and operation that can facilitate grid integration, and proposes a unifying concept—economic carrying capacity—that can provide a framework for evaluating actions to accommodate higher penetrations of RE. There is growing recognition that while technical challenges to variable RE integration are real, they can generally be addressed via a variety of solutions that vary in implementation cost. As a result, limits to RE penetration are primarily economic, driven by factors that include transmission and the flexibility of the power grid to balance supply and demand. This limit can be expressed as economic carrying capacity, or the point at which variable RE is no longer economically competitive or desirable to the system or society.

  7. High-burnup/low-cooling-time fuel carrying capacity of the GA-4 and GA-9 spent fuel shipping casks

    International Nuclear Information System (INIS)

    Boshoven, J.K.; Hopf, J.E.

    1994-01-01

    In response to utilities' projected needs to ship higher burnup spent fuel, General Atomics (GA) has performed shielding and thermal analysis for the GA-4 and GA-9 legal weight shipping casks to determine the minimum cooling times for various burnup levels for fully loaded GA-4 and GA-9 casks and reduced payloads for the casks. Tables are provided in the paper which show the minimum cooling time for a given burnup and payload for each of the casks. The analyses show that the GA-4 and GA-9 casks can carry at least as many high-burnup and/or short-cooling-time spent fuel assemblies as present day shipping casks. In addition, the GA casks are able to carry at least twice as many assemblies as the present day shipping casks if the spent fuel burnup levels and/or cooling times are open-quotes coolerclose quotes or open-quotes as coolclose quotes as their design basis fuels. The increased shipping capacity for these more common open-quotes coolerclose quotes assemblies allows fewer shipments and therefore increases the efficiency and lowers predicted risks of the transport system

  8. Carbon carry capacity and carbon sequestration potential in China based on an integrated analysis of mature forest biomass.

    Science.gov (United States)

    Liu, YingChun; Yu, GuiRui; Wang, QiuFeng; Zhang, YangJian; Xu, ZeHong

    2014-12-01

    Forests play an important role in acting as a carbon sink of terrestrial ecosystem. Although global forests have huge carbon carrying capacity (CCC) and carbon sequestration potential (CSP), there were few quantification reports on Chinese forests. We collected and compiled a forest biomass dataset of China, a total of 5841 sites, based on forest inventory and literature search results. From the dataset we extracted 338 sites with forests aged over 80 years, a threshold for defining mature forest, to establish the mature forest biomass dataset. After analyzing the spatial pattern of the carbon density of Chinese mature forests and its controlling factors, we used carbon density of mature forests as the reference level, and conservatively estimated the CCC of the forests in China by interpolation methods of Regression Kriging, Inverse Distance Weighted and Partial Thin Plate Smoothing Spline. Combining with the sixth National Forest Resources Inventory, we also estimated the forest CSP. The results revealed positive relationships between carbon density of mature forests and temperature, precipitation and stand age, and the horizontal and elevational patterns of carbon density of mature forests can be well predicted by temperature and precipitation. The total CCC and CSP of the existing forests are 19.87 and 13.86 Pg C, respectively. Subtropical forests would have more CCC and CSP than other biomes. Consequently, relying on forests to uptake carbon by decreasing disturbance on forests would be an alternative approach for mitigating greenhouse gas concentration effects besides afforestation and reforestation.

  9. Evaluation of fiber’s misorientation effect on compliance and load carry capacity of shaped composite beams

    Science.gov (United States)

    Polilov, A. N.; Tatus’, N. A.

    2018-04-01

    The goal of this paper is analysis of design methods for composite beams and plates with curvilinear fiber trajectories. The novelty of this approach is determined by the fact that traditional composite materials are typically formed using prepregs with rectilinear fibers only. The results application area is associated with design process for shaped composite structure element by using of biomechanical principles. One of the related problems is the evaluation of fiber’s misorientation effect on stiffness and load carry capacity of shaped composite element with curvilinear fiber trajectories. Equistrong beam with constant cross-section area is considered as example, and it can be produced by unidirectional fiber bunch forming, impregnated with polymer matrix. Effective elastic modulus evaluation methods for structures with curvilinear fiber trajectories are validated. Misorientation angle range (up to 5o) when material with required accuracy can be considered as homogeneous, neglecting fiber misorientation, is determined. It is shown that for the beams with height-to-width ratio small enough it is possible to consider 2D misorientation only.

  10. The carrying capacity and the effects of protection level in three marine protected areas in the Balearic Islands (NW Mediterranean

    Directory of Open Access Journals (Sweden)

    Josep Coll

    2012-08-01

    Full Text Available The monitoring of fisheries target species in three marine protected areas (MPAs located in the NW Mediterranean provided important conclusions on management effects. The abundance, occurrence and biomass of large fish showed consistent and growing trends inside the MPAs. Fish diversity was also favoured by protection. Nevertheless, spatial variance of these indicators at a medium scale was often of the same order as that caused by time between different protection levels. The carrying capacity was determined for the first time in western Mediterranean MPAs in five out of six sites studied, and in all cases it was achieved within the first five years of protection. The observed values indicate that the system of the studied MPAs is representative of the general coastal environment of the Balearic Islands, but is far from the potential of other MPAs which are considered as hot spots in other localities. Likewise, this study shows that partially protected areas can also be fairly effective if their habitats fit with those required by target species and fishing modalities are suitably regulated or banned.

  11. A New Methodology for Solving Trajectory Planning and Dynamic Load-Carrying Capacity of a Robot Manipulator

    Directory of Open Access Journals (Sweden)

    Wanjin Guo

    2016-01-01

    Full Text Available A new methodology using a direct method for obtaining the best found trajectory planning and maximum dynamic load-carrying capacity (DLCC is presented for a 5-degree of freedom (DOF hybrid robot manipulator. A nonlinear constrained multiobjective optimization problem is formulated with four objective functions, namely, travel time, total energy involved in the motion, joint jerks, and joint acceleration. The vector of decision variables is defined by the sequence of the time-interval lengths associated with each two consecutive via-points on the desired trajectory of the 5-DOF robot generalized coordinates. Then this vector of decision variables is computed in order to minimize the cost function (which is the weighted sum of these four objective functions subject to constraints on joint positions, velocities, acceleration, jerks, forces/torques, and payload mass. Two separate approaches are proposed to deal with the trajectory planning problem and the maximum DLCC calculation for the 5-DOF robot manipulator using an evolutionary optimization technique. The adopted evolutionary algorithm is the elitist nondominated sorting genetic algorithm (NSGA-II. A numerical application is performed for obtaining best found solutions of trajectory planning and maximum DLCC calculation for the 5-DOF hybrid robot manipulator.

  12. Using Urban-Carrying Capacity as a Benchmark for Sustainable Urban Development: An Empirical Study of Beijing

    Directory of Open Access Journals (Sweden)

    Yigang Wei

    2015-03-01

    Full Text Available Sustainable urban development has been receiving growing concerns from both city managers and urban residents across the world. As a yardstick of sustainability, urban carrying capacity (UCC is an important conceptual underpinning that guides local governments in promoting sustainable urban development. However, existing studies still lack consensus not only on the theoretical aspects, but also on the methodological issues for UCC monitoring and evaluation. A knowledge gap exists, which this paper fills. This study aims to develop a practical UCC assessment framework to guide urban development towards achieving sustainability. The quantitative-based assessment framework provides a set of measurable indicators and benchmarks for city managers to conduct routine monitoring on progress toward urban sustainability, and helps identify deficient areas, which urgently need resource allocation to improve UCC. Focusing on a case study of Beijing, this study demonstrates that the framework is useful in promoting urban sustainability. This framework provides rich implications for other city prototypes in China as the nation marches into the next phase of development.

  13. Civil Service Human Resource Capacity and Information Technology

    African Journals Online (AJOL)

    Tesfaye

    2009-01-01

    Jan 1, 2009 ... had no impact on the size of jobs that require high-level of human resource capacity. Furthermore ... level human resource capacity has an effect on the size of supervisors, which is the main ...... depreciation. 5 This indicates ...

  14. 基于物元分析理论的旅游承载力研究%Study on Tourist Carrying Capacity Based on Matter Element Analysis

    Institute of Scientific and Technical Information of China (English)

    刘云国

    2005-01-01

    This paper proposes that it is necessary to implement the concept of tourist carrying capacity to facilitate the tourism planning, and presents a method to evaluate the carrying capacity. The method cailed matter element analysis can solve the uncertain a,nd incompatible problem of the evaluated factors in assessing carrying capacity.The current state of a destination s carrying capacity can be determined by establishing the standard indexes and the matter element model. Through the evaluating of the travel industry zones of the Autonomous Prefecture of Western Hunan, the method is proved to be simple and feasible, and it is improved to be significant for the tourism planning and determination as well as the sustainable development of the regional tourism.

  15. 攀枝花市旅游人口承载容量研究%Research of Tourist Population Carrying Capacity in Panzhihua City

    Institute of Scientific and Technical Information of China (English)

    孔博; 邓伟; 刘邵权; 陶和平; 刘颖

    2011-01-01

    选择四川省攀枝花市典型旅游城市5县19个景点作为研究对象,通过分析空间、社会、经济作为三大主要旅游人口容量的影响因子,并改进了社会容量方法,定量分析了攀枝花市旅游人口承载容量。研究表明,空间承载容量成为制约攀枝花市景区发展并限制接待游客容量的主要因素,主要包括旅游线路不发达,景区内可达面积小。本研究有助于丰富和完善旅游人口承载容量的理论和研究体系,可为当地的生态环境保护和旅游开发建设提供科学依据。%Five counties,including 19 scenic spots,which have features of typical tourism city,in Panzhihua city,Sichuan province,were chosen for the study of the tourism carrying capacity.To quantitatively analyze the tourism carrying capacity of the five counties,the three main factors,which were spatial carrying capacity,social carrying capacity,and economic carrying capacity,were studied and the model of the social carrying capacity was improved.The spatial carrying capacity became the main barrier to limit the reception visitor carrying capacity and restrict the development of Panzhihua scenic area,the main reasons included inconvenient tourism route and traffic transportation,the small developed reachable area.It can accelerate the establishment of economic effect and environmental effect of local tourism industry.

  16. Study plan for evaluating carrying capacity: Measure 7.1A of the Northwest Power Planning Council's 1994 Fish and Wildlife Program. Report 2 of 4, Final report

    International Nuclear Information System (INIS)

    Neitzel, D.A.; Johnson, G.E.

    1996-05-01

    This report is one report of four that the Pacific Northwest National Laboratory (PNNL) staff used to address Measure 7.1A in the Northwest Power Planning Council's (Council) fish and Wildlife Program (Program) dated December 1994 (NPPC 1994). Measure 7.1A calls for the Bonneville Power Administration (BPA) to fund an evaluation of salmon survival, ecology, carrying capacity, and limiting factors in freshwater, estuarine, and marine habitats. Additionally, the Measure asks for the development of a study plan based on the critical uncertainties and research needs identified during the evaluation. In the evaluation of carrying capacity the authors concluded that defining capacity and listing the determinants of capacity is not a simple exercise. Capacity is a complex variable among the attributes that all together define salmon performance. To pursue the capacity parameter, that is, a single number or set of numbers that quantify how many salmon the basin or any part of the basin can support, will not lead to the development of a useful study plan. To increase understanding of ecology, carrying capacity, and limiting factors, it is necessary to deal with the complexity of the sustained performance of salmon in the Columbia River Basin. This report describes the elements of a study plan that could be used to increase the understanding of ecology, carrying capacity, and limiting factors that influence salmon survival under current conditions

  17. Impact of the thermal effect on the load-carrying capacity of a slipper pair for an aviation axial-piston pump

    Directory of Open Access Journals (Sweden)

    Hesheng TANG

    2018-02-01

    Full Text Available A thermal hydraulic model based on the lumped parameter method is presented to analyze the load-carrying capacity of a slipper pair in an aviation axial-piston pump under specified operating conditions. Both theoretical and experimental results are presented to demonstrate the validity of the thermal hydraulic model. The results illustrate that the squeezing force and thermal wedge bearing force are the main factors that affect the film thickness and load-carrying capacity. At high oil temperature and high load pressure, the film thickness decreases with increasing clamping force due to a combined action of the squeezing bearing force and the thermal wedge bearing force, but the load-carrying capacity will increase. An increase of the film thickness is proven to be beneficial under high shaft rotational speed but especially dangerous as it strongly increases the ripple amplitude of the film thickness, which leads to decreasing the load-carrying capacity. The structural parameters of the slipper can be optimized to achieve desired performance, such as the slipper radius ratio and orifice length diameter ratio. To satisfy the requirement of the load-carrying capacity, the slipper radius ratio should be selected from 1.4 to 1.8, and the orifice length diameter ratio should be selected from 4 to 5. Keywords: Aviation axial piston pump, Fluid lubrication, Load-carrying capacity, Slipper pair, Thermal effect

  18. Human milk metagenome: a functional capacity analysis

    Science.gov (United States)

    2013-01-01

    Background Human milk contains a diverse population of bacteria that likely influences colonization of the infant gastrointestinal tract. Recent studies, however, have been limited to characterization of this microbial community by 16S rRNA analysis. In the present study, a metagenomic approach using Illumina sequencing of a pooled milk sample (ten donors) was employed to determine the genera of bacteria and the types of bacterial open reading frames in human milk that may influence bacterial establishment and stability in this primal food matrix. The human milk metagenome was also compared to that of breast-fed and formula-fed infants’ feces (n = 5, each) and mothers’ feces (n = 3) at the phylum level and at a functional level using open reading frame abundance. Additionally, immune-modulatory bacterial-DNA motifs were also searched for within human milk. Results The bacterial community in human milk contained over 360 prokaryotic genera, with sequences aligning predominantly to the phyla of Proteobacteria (65%) and Firmicutes (34%), and the genera of Pseudomonas (61.1%), Staphylococcus (33.4%) and Streptococcus (0.5%). From assembled human milk-derived contigs, 30,128 open reading frames were annotated and assigned to functional categories. When compared to the metagenome of infants’ and mothers’ feces, the human milk metagenome was less diverse at the phylum level, and contained more open reading frames associated with nitrogen metabolism, membrane transport and stress response (P milk metagenome also contained a similar occurrence of immune-modulatory DNA motifs to that of infants’ and mothers’ fecal metagenomes. Conclusions Our results further expand the complexity of the human milk metagenome and enforce the benefits of human milk ingestion on the microbial colonization of the infant gut and immunity. Discovery of immune-modulatory motifs in the metagenome of human milk indicates more exhaustive analyses of the functionality of the human

  19. 楼观台森林公园旅游环境容量研究%Tourism Environmental Carrying Capacity of Louguantai Forest Park

    Institute of Scientific and Technical Information of China (English)

    张晓慧; 陈强

    2011-01-01

    综合旅游空间容量、旅游生态容量、旅游设施容量、旅游心理容量4个因素,对楼观台国家森林公园旅游环境容量进行了测算,通过对比发现楼观台森林公园游客接待量远小于理论旅游环境容量,并对造成这种现状的主要原因进行了分析,提出了相关建议.%In this paper, an integrated estimation on the tourism environmental carrying capacity of Louguantai National Forest Park was made from the aspects of ecological carrying capacity, spatial carrying capacity, facility carrying capacity, and psychological carrying capacity. It was found that the number of reception tourists in the park was far less than its theoretical predication tourism environment capacity. Main reasons were analysised and some suggestions were proposed.

  20. Evaluation of carrying capacity: Measure 7.1A of the Northwest Power Planning Council's 1994 Fish and Wildlife Program. Report 1 of 4, Final report

    International Nuclear Information System (INIS)

    Neitzel, D.A.; Johnson, G.E.

    1996-05-01

    This report is one of four that the Pacific Northwest National Laboratory (PNNL) staff prepared to address Measure 7.1A in the Northwest Power Planning Council's (Council) Fish and Wildlife Program (Program) dated december 1994 (NPPC 1994). Measure 7.1A calls for the Bonneville Power Administration (BPA) to fund an evaluation of salmon survival, ecology, carrying capacity, and limiting factors in freshwater, estuarine, and marine habitats. Additionally, the Measure asks for development of a study plan based on critical uncertainties and research needs identified during the evaluation. This report deals with the evaluation of carrying capacity. It describes the analysis of different views of capacity as it relates to salmon survival and abundance. The report ends with conclusions and recommendations for studying carrying capacity

  1. Evaluation of Carrying Capacity : Measure 7.1A of the Northwest Power Planning Council`s 1994 Fish and Wildlife Program : Report 1 of 4, Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Neitzel, Duane A.; Johnson, Gary E.

    1996-05-01

    This report is one of four that the Pacific Northwest National Laboratory (PNNL) staff prepared to address Measure 7.1A in the Northwest Power Planning Council's (Council) Fish and Wildlife Program (Program) dated december 1994 (NPPC 1994). Measure 7.1A calls for the Bonneville Power Administration (BPA) to fund an evaluation of salmon survival, ecology, carrying capacity, and limiting factors in freshwater, estuarine, and marine habitats. Additionally, the Measure asks for development of a study plan based on critical uncertainties and research needs identified during the evaluation. This report deals with the evaluation of carrying capacity. It describes the analysis of different views of capacity as it relates to salmon survival and abundance. The report ends with conclusions and recommendations for studying carrying capacity.

  2. Construction of a novel lentiviral vector carrying human B-domain ...

    African Journals Online (AJOL)

    ... integration were detected in all cell lines after transfection. A novel lentiviral vector carrying human FVIII³BD was constructed, which was able to transfect different mammalian cell types accompanied by high-level activity. This lentiviral vector may provide a theoretical basis for the gene therapy of patients with hemophilia ...

  3. GIS and agent based spatial-temporal simulation modeling for assessing tourism social carrying capacity: a study on Mount Emei scenic area, China

    Science.gov (United States)

    Zhang, Renjun

    2007-06-01

    Each scenic area can sustain a specific level of acceptance of tourist development and use, beyond which further development can result in socio-cultural deterioration or a decline in the quality of the experience gained by visitors. This specific level is called carrying capacity. Social carrying capacity can be defined as the maximum level of use (in terms of numbers and activities) that can be absorbed by an area without an unacceptable decline in the quality of experience of visitors and without an unacceptable adverse impact on the society of the area. It is difficult to assess the carrying capacity, because the carrying capacity is determined by not only the number of visitors, but also the time, the type of the recreation, the characters of each individual and the physical environment. The objective of this study is to build a spatial-temporal simulation model to simulate the spatial-temporal distribution of tourists. This model is a tourist spatial behaviors simulator (TSBS). Based on TSBS, the changes of each visitor's travel patterns such as location, cost, and other states data are recoded in a state table. By analyzing this table, the intensity of the tourist use in any area can be calculated; the changes of the quality of tourism experience can be quantized and analyzed. So based on this micro simulation method the social carrying capacity can be assessed more accurately, can be monitored proactively and managed adaptively. In this paper, the carrying capacity of Mount Emei scenic area is analyzed as followed: The author selected the intensity of the crowd as the monitoring Indicators. it is regarded that longer waiting time means more crowded. TSBS was used to simulate the spatial-temporal distribution of tourists. the average of waiting time all the visitors is calculated. And then the author assessed the social carrying capacity of Mount Emei scenic area, found the key factors have impacted on social carrying capacity. The results show that the TSBS

  4. Does recombinant human Epo increase exercise capacity by means other than augmenting oxygen transport?

    DEFF Research Database (Denmark)

    Lundby, C; Robach, P; Boushel, R

    2008-01-01

    This study was performed to test the hypothesis that administration of recombinant human erythropoietin (rHuEpo) in humans increases maximal oxygen consumption by augmenting the maximal oxygen carrying capacity of blood. Systemic and leg oxygen delivery and oxygen uptake were studied during...... before rHuEpo treatment). Blood buffer capacity remained unaffected by rHuEpo treatment and hemodilution. The augmented hematocrit did not compromise peak cardiac output. In summary, in healthy humans, rHuEpo increases maximal oxygen consumption due to augmented systemic and muscular peak oxygen delivery....

  5. Theory Analysis and Experiment Research of the Leg Mechanism for the Human-Carrying Walking Chair Robot

    Directory of Open Access Journals (Sweden)

    Lingfeng Sang

    2014-01-01

    Full Text Available For the high carrying capacity of the human-carrying walking chair robot, in this paper, 2-UPS+UP parallel mechanism is selected as the leg mechanism; then kinematics, workspace, control, and experiment of the leg mechanism are researched in detail. Firstly, design of the whole mechanism is described and degrees of freedom of the leg mechanism are analyzed. Second, the forward position, inverse position, and velocity of leg mechanism are studied. Third, based on the kinematics analysis and the structural constraints, the reachable workspace of 2-UPS+UP parallel mechanism is solved, and then the optimal motion workspace is searched in the reachable workspace by choosing the condition number as the evaluation index. Fourth, according to the theory analysis of the parallel leg mechanism, its control system is designed and the compound position control strategy is studied. Finally, in optimal motion workspace, the compound position control strategy is verified by using circular track with the radius 100 mm; the experiment results show that the leg mechanism moves smoothly and does not tremble obviously. Theory analysis and experiment research of the single leg mechanism provide a theoretical foundation for the control of the quadruped human-carrying walking chair robot.

  6. Carrying capacity and tourism planning. case study at Praia Brava- Itajai, in face to the settlement of Canto da Brava Resort

    Directory of Open Access Journals (Sweden)

    Doris Van de Meene Ruschmann

    2008-05-01

    Full Text Available Sustainable tourism presumes cultural authenticity, social inclusion, conservation of natural resources and service quality. Those are major issues standing for economical viability in the long run. This article analyzes carrying capacity in Praia Brava, Itajai/SC, evaluating environmental comfort for dwellers as well as for users of the beach building future scenarios as Canto da Brava resort is settled. Tourism carrying capacity is tied to methods of identification and evaluation of environmental indicators. In this case, Cifuentes Method was chosen because it integrates physical, biotical and infra structural factors, through assessment of physical, real and effective carrying capacity. Quantitative results have been considered analyzing impacts and tolerable limits of change taking into account users perception.

  7. 承德市旅游环境承载力分析%Analysis of Tourism Environment Carrying Capacity of Chengde City

    Institute of Scientific and Technical Information of China (English)

    贾紫牧; 曾维华

    2016-01-01

    With the rapid expansion of domestic tourism demands,the number of tourists had increased dramatically.Relatively poor facilities and services led to overload of the tourism environment carrying capacity.Eleven most attractive scenic spots in Chengde City were chosen to quantitatively analyze resource carrying capacity,environment carrying capacity and psychological carrying capacity.Then,tourism environment carrying capacity of Chengde City was calculated by use of Buckets effect.The results showed that carrying capacity of the tourism environment of Chengde City was about 29.93 million tourists per year and almost reached to saturation with the increase of tourists year by year.%我国旅游需求快速膨胀,游客数量急剧增加,但配套设施及管理、服务水平相对落后,旅游环境超负荷承栽.选择承德市11个最具吸引力的景区,综合考虑资源、环境和心理3个维度,定量分析各承载力分量,在此基础上根据“木桶原理”确定承德市旅游环境承载力.研究表明:承德市全年的旅游环境承栽力约为2993万人,随着游客人数逐年递增,旅游环境承载力已接近饱和.

  8. Potential for Grey wolf Canis lupus in the Netherlands : effects of habitat fragmentation and climate change on the carrying capacity and population dynamics

    NARCIS (Netherlands)

    Potiek, A.; Wamelink, G.W.W.; Jochem, R.; Langevelde, van F.

    2012-01-01

    Recolonization of the Netherlands by wolves is likely to occur within 5 to 10 years, and for management reasons the habitat suitability should be understood. Therefore, I predicted the carrying capacity and population dynamics of the wolf in the Netherlands, and studied the effects of habitat

  9. Overview of the Domestic Research on Tourism Carrying Capacity%国内旅游容量研究述评

    Institute of Scientific and Technical Information of China (English)

    寇文波; 李德云; 秦愿; 鲁梦

    2017-01-01

    旅游饱和、旅游超载等现象频出,旅游容量及相关研究日益成为学界和业界共同关注的焦点.本文利用文献分析法,检索、梳理并评析国内旅游容量研究的相关文献,围绕旅游容量的概念、内涵、研究成果以及面临的挑战等内容进行分类归纳.研究发现,国内旅游容量研究侧重于概念、内涵解析以及旅游景区管理面临的挑战等方面,未来应侧重于完善概念体系、改进计算方法、融入可持续发展理念以及实际应用方面的研究,以期本文的研究能为今后国内相关研究和旅游地管理实践提供参考和借鉴.%The phenomenon of tourism saturation and tourism overload frequently happens,which increases the attention of academic and tourism destination managers.This paper retrieves and analyzes the relevant literatures on domestic tourism capacity research,and summarizes the contents of tourism capacity,connotation,research results and challenges.It is found that the domestic tourism capacity research should focus on the conceptual system,the connotation analysis and the challenge of tourism scenic spot management.The future should focus on perfecting the conceptual system,improving the calculation method,integrating into the concept of sustainable development and practical research.This research can provide reference for the future domestic research and tourism management practice.

  10. 福清旅游环境承载力研究%Study on the Carrying Capacity of Fuqing Tourism Environment

    Institute of Scientific and Technical Information of China (English)

    姜玲

    2012-01-01

    The policy of developing the economic zone on the west side of Taiwan strait has created a good opportunity for the development of tourism in Fujian. Due to its favorable geographical location, Fuqing will definitely become the beneficiary of the development. A significant growth in tourism is foreseeable. This paper analyzes the carrying capacity of Fuqing ecological environment, carrying capacity of resources space, residents' psychological carrying capacity as well as economic carrying capacity so as to find out the comprehensive environment carrying capacity. The obstacles to the development of tourism in Fuqing have been identified and countermeasures were provided in the hope of promoting Fuqing tourism industry.%海西建设的提出为福建旅游业发展带来了良好的契机,福清由于其优越的地理位置,势必会成为海西建设的受益者,旅游业必将会得到迅猛的发展,在这种前提下,本文通过对福清市的生态环境承载力、资源空间承载力、居民心理承载力和经济承载力的定量分析,得出了福清市的综合环境承载力,从而找出制约福清旅游业发展的主要因素,在此基础上提出相应的对策建议,以此来促进福清旅游业的发展。

  11. Physical inactivity and muscle oxidative capacity in humans.

    Science.gov (United States)

    Gram, Martin; Dahl, Rannvá; Dela, Flemming

    2014-01-01

    Physical inactivity is associated with a high prevalence of type 2 diabetes and is an independent predictor of mortality. It is possible that the detrimental effects of physical inactivity are mediated through a lack of adequate muscle oxidative capacity. This short review will cover the present literature on the effects of different models of inactivity on muscle oxidative capacity in humans. Effects of physical inactivity include decreased mitochondrial content, decreased activity of oxidative enzymes, changes in markers of oxidative stress and a decreased expression of genes and contents of proteins related to oxidative phosphorylation. With such a substantial down-regulation, it is likely that a range of adenosine triphosphate (ATP)-dependent pathways such as calcium signalling, respiratory capacity and apoptosis are affected by physical inactivity. However, this has not been investigated in humans, and further studies are required to substantiate this hypothesis, which could expand our knowledge of the potential link between lifestyle-related diseases and muscle oxidative capacity. Furthermore, even though a large body of literature reports the effect of physical training on muscle oxidative capacity, the adaptations that occur with physical inactivity may not always be opposite to that of physical training. Thus, it is concluded that studies on the effect of physical inactivity per se on muscle oxidative capacity in functional human skeletal muscle are warranted.

  12. Water ecological carrying capacity of urban lakes in the context of rapid urbanization: A case study of East Lake in Wuhan

    Science.gov (United States)

    Ding, Lei; Chen, Kun-lun; Cheng, Sheng-gao; Wang, Xu

    With the excessive development of social economy, water scarcity and water environment deterioration become a common phenomenon in metropolis. As a crucial component of urban water environment system, urban lake is mainly influenced by social economic system and tourism system. In this paper, a framework for quantitatively evaluating development sustainability of urban lake was established by a multi-objective model that represented water ecological carrying capacity (WECC). And nine key indicators including population, irrigation area, tourist quantity, the average number of hotel daily reception, TP, TN, CODMn, BOD5 were chosen from urban social-economy system and natural resilience aspects, with their index weight was determined by using the Structure Entropy Weight method. Then, we took Wuhan East Lake, the largest urban lake in China as a case study, and selected five time sections including 2002, 2004, 2007, 2009 and 2012 to synthetically evaluate and comparatively analyze the dynamic change of WECC. The results showed that: firstly, the water ecological carrying capacity values of the East Lake in five time sections were 1.17, 1.07, 1.64, 1.53 and 2.01 respectively, which all exceeded 1 and increased fluctuation. The rapid growth of population and GDP lead to sharply increasing demand for water quantity. However, a large amount of the domestic sewage and industrial waste led by economic development increases pressure on ecological environment of urban lakes. Secondly, the carrying capacity of the East Lake for tourist activities was still low. The value in 2012 was only 0.22, keeping at a slowly increasing phase, which indicates that the East Lake has large opportunity and space for developing the water resource carrying capacity and could make further efforts to attract tourists. Moreover, the WECC of the East Lake was mainly affected by rapid social and economic development and water environment damage caused by organic pollutants. From the view of urban

  13. Limited communication capacity unveils strategies for human interaction

    Science.gov (United States)

    Miritello, Giovanna; Lara, Rubén; Cebrian, Manuel; Moro, Esteban

    2013-06-01

    Connectivity is the key process that characterizes the structural and functional properties of social networks. However, the bursty activity of dyadic interactions may hinder the discrimination of inactive ties from large interevent times in active ones. We develop a principled method to detect tie de-activation and apply it to a large longitudinal, cross-sectional communication dataset (~19 months, ~20 million people). Contrary to the perception of ever-growing connectivity, we observe that individuals exhibit a finite communication capacity, which limits the number of ties they can maintain active in time. On average men display higher capacity than women, and this capacity decreases for both genders over their lifespan. Separating communication capacity from activity reveals a diverse range of tie activation strategies, from stable to exploratory. This allows us to draw novel relationships between individual strategies for human interaction and the evolution of social networks at global scale.

  14. Motor adaptation capacity as a function of age in carrying out a repetitive assembly task at imposed work paces.

    Science.gov (United States)

    Gilles, Martine Annie; Guélin, Jean-Charles; Desbrosses, Kévin; Wild, Pascal

    2017-10-01

    The working population is getting older. Workers must adapt to changing conditions to respond to the efforts required by the tasks they have to perform. In this laboratory-based study, we investigated the capacities of motor adaptation as a function of age and work pace. Two phases were identified in the task performed: a collection phase, involving dominant use of the lower limbs; and an assembly phase, involving bi-manual motor skills. Results showed that senior workers were mainly limited during the collection phase, whereas they had less difficulty completing the assembly phase. However, senior workers did increase the vertical force applied while assembling parts, whatever the work pace. In younger and middle-aged subjects, vertical force was increased only for the faster pace. Older workers could adapt to perform repetitive tasks under different time constraints, but adaptation required greater effort than for younger workers. These results point towards a higher risk of developing musculoskeletal disorders among seniors. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. An Applied Method for Predicting the Load-Carrying Capacity in Compression of Thin-Wall Composite Structures with Impact Damage

    Science.gov (United States)

    Mitrofanov, O.; Pavelko, I.; Varickis, S.; Vagele, A.

    2018-03-01

    The necessity for considering both strength criteria and postbuckling effects in calculating the load-carrying capacity in compression of thin-wall composite structures with impact damage is substantiated. An original applied method ensuring solution of these problems with an accuracy sufficient for practical design tasks is developed. The main advantage of the method is its applicability in terms of computing resources and the set of initial data required. The results of application of the method to solution of the problem of compression of fragments of thin-wall honeycomb panel damaged by impacts of various energies are presented. After a comparison of calculation results with experimental data, a working algorithm for calculating the reduction in the load-carrying capacity of a composite object with impact damage is adopted.

  16. Infrared A radiation promotes survival of human melanocytes carrying ultraviolet radiation-induced DNA damage.

    Science.gov (United States)

    Kimeswenger, Susanne; Schwarz, Agatha; Födinger, Dagmar; Müller, Susanne; Pehamberger, Hubert; Schwarz, Thomas; Jantschitsch, Christian

    2016-06-01

    The link between solar radiation and melanoma is still elusive. Although infrared radiation (IR) accounts for over 50% of terrestrial solar energy, its influence on human skin is not well explored. There is increasing evidence that IR influences the expression patterns of several molecules independently of heat. A previous in vivo study revealed that pretreatment with IR might promote the development of UVR-induced non-epithelial skin cancer and possibly of melanoma in mice. To expand on this, the aim of the present study was to evaluate the impact of IR on UVR-induced apoptosis and DNA repair in normal human epidermal melanocytes. The balance between these two effects is a key factor of malignant transformation. Human melanocytes were exposed to physiologic doses of IR and UVR. Compared to cells irradiated with UVR only, simultaneous exposure to IR significantly reduced the apoptotic rate. However, IR did not influence the repair of UVR-induced DNA damage. IR partly reversed the pro-apoptotic effects of UVR via modification of the expression and activity of proteins mainly of the extrinsic apoptotic pathway. In conclusion, IR enhances the survival of melanocytes carrying UVR-induced DNA damage and thereby might contribute to melanomagenesis. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  17. Methodology for calculating the tourist carrying capacity as a tool for environmental management and its implementation in five northern Colombian Caribbean beaches

    International Nuclear Information System (INIS)

    Botero, Camilo; Yuri, Hurtado; Gonzalez, Jose

    2008-01-01

    This paper presents a new methodology to calculate carrying capacity in tourist beaches, further than merely environmental issues. Moreover, it understands beaches as complex systems towards its sustainable development. Five beaches in the North Caribbean coast of Colombia were chosen and classified in four tourism beach sorts: intensive, conservation, shared and ethnic. The analysis was done with legal framework review, fieldwork and indicators design, within three components: environmental support, urban infrastructure and tourist services. A new model to calculate carrying capacity in tourist beaches was created, and later applied on the study beaches. Current conditions of the five beaches were highlighted, their tourist carrying capacity were calculated and more important actions in each component were recommended. The main conclusion foster to take in consideration natural conditions as a core factor in beach management, but including a holistic approach in making decision process. Also this paper showed the current conditions of Colombian beaches as a warning, giving recommendations in short and medium term. This document is result of the project Determinacion de un sistema de calificacion y certificacion de playas turisticas.

  18. Study on quantitative evaluation of environmental carrying capacity of eco-tourism%生态旅游环境承载力评价研究

    Institute of Scientific and Technical Information of China (English)

    孙金梅; 林建

    2012-01-01

    近年来生态旅游迅猛发展,成为国内外旅游的热点,但生态旅游环境压力加大,如何评价环境压力就成了不容忽视的问题。在系统分析法基础上,提出生态旅游环境承载力概念,建立一套包括经济和社会承载力等5个子系统的科学生态旅游环境承载力指标体系及评价模型,并以武夷山自然保护区为例进行实证研究,研究结果表明其环境承载力远远超过实际游客数量,需要采取加强生态监测和科学功能分区等措施。%In recent years,ecological tourism becomes the domestic and foreign tourist hot spot,but the pressure of ecological tourism environment is increased. How to evaluate the environmental stress becomes a problem that cannot ignored. Combined with the former research,based on the system analysis method, the concepts of the ecological carrying capacity of tourism environment is proposed,a set of scientific ecological carrying capacity of tourism environment index system and evaluation model are established, including five sub-systems of economic and social capacity,and taking the nature protection area of Wuyishan as a case study, the results shows that the environmental carrying capacity force is far more than the actual number of tourists. It is needed to take the measures of strengthening the ecological monitoring and scientific zoning.

  19. Tourism Planning and Design Based on the Environmental Carrying Capacity%基于环境承载力的旅游规划设计

    Institute of Scientific and Technical Information of China (English)

    冀慧萍

    2015-01-01

    旅游的发展必然会对环境造成一定的影响,如何在发展旅游与保护环境之间取得平衡,是旅游规划设计中必须考虑的一个焦点问题. 环境承载能力又称环境容量,基于环境承载力进行旅游规划设计,能很好地确保旅游与环境间的和谐发展. 笔者对环境承载力进行了简要概述,提出了基于环境自洁能力、基于环境再生能力、基于生态足迹的旅游环境承载力的计算方法. 就基于环境承载能力的旅游规划设计进行了探讨,对地区旅游业的可持续发展、旅游资源的开发和调整、旅游环境的保护具有重要意义.%The development of tourism causes certain effect on the environment, and how to achieve a balance between tourism development and environmental protection is a focus problem, which in the tourism planning and design must be considered.Environmental carrying capacity is also called the environment capacity, tourism planning and design based on the environmental carrying capacity can ensure the harmonious development between tourism and environment.In this pa-per, tourism planning and design based on the environmental carrying capacity are discussed, which has positive signifi-cance for the sustainable development of regional tourism experience, the development of tourism resources and the adjust-ment, the protection of tourism environment .

  20. Physical inactivity and muscle oxidative capacity in humans

    DEFF Research Database (Denmark)

    Gram, Martin; Dahl, Rannvá; Dela, Flemming

    2014-01-01

    Physical inactivity is associated with a high prevalence of type 2 diabetes and is an independent predictor of mortality. It is possible that the detrimental effects of physical inactivity are mediated through a lack of adequate muscle oxidative capacity. This short review will cover the present...... literature on the effects of different models of inactivity on muscle oxidative capacity in humans. Effects of physical inactivity include decreased mitochondrial content, decreased activity of oxidative enzymes, changes in markers of oxidative stress and a decreased expression of genes and contents...... of proteins related to oxidative phosphorylation. With such a substantial down-regulation, it is likely that a range of adenosine triphosphate (ATP)-dependent pathways such as calcium signalling, respiratory capacity and apoptosis are affected by physical inactivity. However, this has not been investigated...

  1. Direct FEM-computation of load carrying capacity of highly loaded passive components; Direkte FEM - Berechnung der Tragfaehigkeit hochbeanspruchter passiver Komponenten

    Energy Technology Data Exchange (ETDEWEB)

    Staat, M; Heitzer, M [Forschungszentrum Juelich GmbH (Germany). Inst. fuer Sicherheitsforschung und Reaktortechnik

    1998-11-01

    Detailed, inelastic FEM analyses yield accurate information about the stresses and deformations in passive components. The local loading conditions, however, cannot be directly compared with a limit load in terms of structural mechanics. Concentration on the load carrying capacity is an approach simplifying the analysis. Based on the plasticity theory, limit and shakedown analyses calculate the load carrying capacities directly and exactly. The paper explains the implementation of the limit and shakedown data sets in a general FEM program and the direct calculation of the load carrying capacities of passive components. The concepts used are explained with respect to common structural analysis. Examples assuming high local stresses illustrate the application of FEM-based limit and shakedown analyses. The calculated interaction diagrams present a good insight into the applicable operational loads of individual passive components. The load carrying analysis also opens up a structure mechanics-based approach to assessing the load-to-collapse of cracked components made of highly ductile fracture-resistant material. (orig./CB) [Deutsch] Genaue Kenntnis der Spannungen und Verformungen in passiven Komponenten gewinnt man mit detailierten inelastischen FEM Analysen. Die lokale Beanspruchung laesst sich aber nicht direkt mit einer Beanspruchbarkeit im strukturmechanischen Sinne vergleichen. Konzentriert man sich auf die Frage nach der Tragfaehigkeit, dann vereinfacht sich die Analyse. Im Rahmen der Plastizitaetstheorie berechnen Traglast- und Einspielanalyse die tragbaren Lasten direkt und exakt. In diesem Beitrag wird eine Implementierung der Traglast- und Einspielsaetze in ein allgemeines FEM Programm vorgestellt, mit der die Tragfaehigkeit passiver Komponenten direkt berechnet wird. Die benutzten Konzepte werden in Bezug auf die uebliche Strukturanalyse erlaeutert. Beispiele mit lokal hoher Beanspruchung verdeutlichen die Anwendung der FEM basierten Traglast- und

  2. Problem in application carrying capacity approach for land allocation assessment in Indonesian municipal spatial planning: A case of Kutai Kartanegara Regency

    Science.gov (United States)

    Wijaya, I. N. S.; Rahadi, B.; Lusiana, N.; Maulidina, I.

    2017-06-01

    Urbanization in many countries, such as Indonesia, is commonly appeared as a dynamic population of developed areas. It is followed with reducing rural uses of land for improving urban land uses such as housing, industry, infrastructure, etc. in response to the growth of population. One may not be sufficiently considered by the urban planners and the decision makers, urbanization also means escalation of natural resources consumption that should be supported by the natural capacity of the area. In this situation, balancing approach as carrying capacity calculation in spatial planning is needed for sustainability. Indonesian Spatial Planning Law 26/2007 has already expressed about the balance approach in the system. Moreover, it strictly regulates the assessment and the permission system in controlling land development, especially for the conversion. However, the reductions over the rural uses of land, especially agriculture, are continuously occurred. Concerning the planning approach, this paper aims to disclose common insufficiency of carrying capacity considerations in Indonesian spatial planning practice. This paper describes common calculation weaknesses in projecting area for the urban development by recalculating the actual gap between supply and demand of agriculture land areas. Here, municipal spatial plan of Kutai Kartanegara Regency is utilized as single sample case to discuss. As the result, the recalculation shows that: 1) there are serious deficit status of agriculture land areas in order to fulfil the demanded agriculture production for the existed population, 2) some calculation of agriculture production may be miss-interpreted because of insufficient explanation toward the productivity of each agriculture commodity.

  3. Carrying capacity of coral reefs

    Digital Repository Service at National Institute of Oceanography (India)

    Wafar, M.V.M.

    The sustainable yield of a commercially exploited fishery is assessed by the biological and environmental factors (including fishing effort). These parameters with a reef are vastly diverse-size, location, species diversity, productivity type...

  4. Development of urbanization in arid and semi arid regions based on the water resource carrying capacity -- a case study of Changji, Xinjiang

    Science.gov (United States)

    Xiao, H.; Zhang, L.; Chai, Z.

    2017-07-01

    The arid and semiarid region in China where have a relatively weak economic foundation, independent development capacity, and the low-level of urbanization. The new urbanization within these regions is facing severe challenges brought by the constraints of resources. In this paper, we selected the Changji Hui Autonomous Prefecture, Xinjiang Uyghur Autonomous Region as study area. We found that agricultural planting structure is the key water consumption index based on the research about the main water demands of domestic, agriculture and industry. Finally, we suggest that more attentions should be paid to the rational utilization of water resources, population carrying capacity, and adjust and upgrade the industrial structure, with the purpose of coordination with the Silk Road Economic Belt.

  5. Human Factors Evaluation of the Modular Lightweight Load-Carrying Equipment (MOLLE) System

    National Research Council Canada - National Science Library

    Sampson, James

    2001-01-01

    .... The FEA was used in drafting a new user requirements document and initiating the development of a modular load-carrying system which ultimately became known as the Modular Lightweight Load-carrying Equipment (MOLLE...

  6. Research on Eco-environment Carrying Capacity of Nanyi Valley Scenic Area%南伊沟景区生态环境承载力研究

    Institute of Scientific and Technical Information of China (English)

    包杰; 杨小林; 王忠斌

    2015-01-01

    Eco-environment carrying capacity of Nanyi Valley Scenic Area in Milin County,Linzhi area,Tibet was studied, the results showed that the recent ecological footprint of the scenic area was 674.70 hm2,effective ecological carrying capacity was 841.13 hm2,and ecological surplus was 166.43 hm2. In terms of current and short-term planning of the scenic area,tourism activities will not bring serious damages to the ecological environment,and ecological footprint of the study area can be further developed. But from the perspective of ecological carrying capacity,the scenic area needs a proper long-term planning,so the paper proposes the sustainable development strategies for the Nanyi Valley Scenic Area. It provides the theoretic basis for the reasonable planning,effective management and sustainable development of the scenic area.%对西藏林芝地区米林县南伊沟景区进行生态环境承载力研究,结果表明,近期南伊沟景区生态足迹量为674.70 hm2,有效生态承载力841.13 hm2,生态盈余166.43 hm2。就景区目前及近期规划来说,开展旅游活动不会对景区生态环境产生大的影响,景区生态足迹还可进一步发展。但从生态承载力方面考虑,景区应特别注意远期规划。提出了南伊沟景区可持续发展对策。

  7. African dust carries microbes across the ocean: are they affecting human and ecosystem health?

    Science.gov (United States)

    Kellogg, Christina A.; Griffin, Dale W.

    2003-01-01

    Atmospheric transport of dust from northwest Africa to the western Atlantic Ocean region may be responsible for a number of environmental hazards, including the demise of Caribbean corals; red tides; amphibian diseases; increased occurrence of asthma in humans; and oxygen depletion (eutrophication) in estuaries. Studies of satellite images suggest that hundreds of millions of tons of dust are trans-ported annually at relatively low altitudes across the Atlantic Ocean to the Caribbean Sea and southeastern United States. The dust emanates from the expanding Sahara/Sahel desert region in Africa and carries a wide variety of bacteria and fungi. The U.S. Geological Survey, in collaboration with the NASA/Goddard Spaceflight Center, is conducting a study to identify microbes--bacteria, fungi, viruses--transported across the Atlantic in African soil dust. Each year, millions of tons of desert dust blow off the west African coast and ride the trade winds across the ocean, affecting the entire Caribbean basin, as well as the southeastern United States. Of the dust reaching the U.S., Florida receives about 50 percent, while the rest may range as far north as Maine or as far west as Colorado. The dust storms can be tracked by satellite and take about one week to cross the Atlantic.

  8. Dynamic Management of Carrying Capacity in Mountain Heritage%山岳型遗产地环境容量动态管理研究

    Institute of Scientific and Technical Information of China (English)

    王德刚; 赵建峰; 黄潇婷

    2015-01-01

    管理的安全可控。%Assessment and management of heritage carrying capacity involve many disciplines. Traditional static measurements obtain the optimal value and saturation value for a certain space, which is the main technical method of traditional carrying capacity study. However, tourists, the subjects who affect carrying capacity of environment are dynamic. Especially, as to Mountain heritage sites, its environmental capacity are relatively limited for their topography characteristics. Massive and concentrated visits will not only increase the pressure of environmental protection, but also influence the quality of tourist experience, and even sometimes threaten tourist physical security. Traditional researches on tourism environmental capacity mainly depend on static measurement. By introducing time dimension and analyzing the rule of spatiotemporal distribution of tourist flow, this paper proposed a new method of dynamic management of carrying capacity in mountain heritage through putting forward the concept of “the spatiotemporal bayonet of tourist flow”, and recognizes it with time geography method. The results of data analysis of Mountain Tai show that there are seven spatial bayonets, which are Red Gate, Stone Valley, Half Way Gate to Heaven, South Gate to Heaven, Tianjie, Jade Emperor Peak and Riguan peak, while the temporal bayonets are decentralization relatively. All those temporal-spatial bayonets of the middle route in Mountain Tai only account for 5. 52% of the whole area, which attract almost 50. 04% tourists. Hence one can see that, recognition and management of Space-Time Bayonet of tourists flow is quite important to dynamic management of carrying capacity in mountain heritage. Besides, this paper discussed the academic contribution of the spatiotemporal bayonet of tourist flow and its potential application practically through regulating tourist ’s behavior, controlling tourism environmental capacity dynamically and building intelligent early-warning system three aspect

  9. 长治湿地公园生态旅游环境容量研究%Study on the Ecotourism Carrying Capacity of Changzhi Wetland Park

    Institute of Scientific and Technical Information of China (English)

    宋珂; 樊正球; 信欣; 王新军; 王原

    2011-01-01

    生态旅游环境容量作为衡量旅游环境与旅游发展是否协调的重要尺度,为旅游的可持续发展提供了较为客观的判断依据.从长治湿地公园自身的承受能力出发,综合旅游生态容量(Ce)与旅游空间容量(Cs)两方面因素评估区域的生态旅游环境容量(其中Ce为40 632~52 366(人次/d),Cs为28 872~55 376(人次/d),对长治湿地公园的规划建设和管理提出3个不同的情景方案.%Ecotourism carrying capacity is a standard to measure the coordination between tourism environment and tourism development.It provides a basis judgment for the sustainable development of tourism.Based on the analyses of the ecological environmental carrying capacity(Ce)and the ecological spatial carrying capacity(Cs),an integrated estimation on the carrying capacity of Changzhi Wetland Park was made(In which Ce is 40632—52366 passengers/day,Cs is 28872—55376 passengers/day).Finally,three scenario programs were put forward,which could be of practical and instructive significances in the planning and management of this wetland park.

  10. Giant panda (Ailuropoda melanoleuca) population dynamics and bamboo (subfamily Bambusoideae) life history: a structured population approach to examining carrying capacity when the prey are semelparous

    Science.gov (United States)

    Carter, J.; Ackleh, A.S.; Leonard, B.P.; Wang, Hongfang

    1999-01-01

    The giant panda, Ailuropoda melanoleuca, is a highly specialized Ursid whose diet consists almost entirely of various species of bamboo. Bamboo (Bambusoideae) is a grass subfamily whose species often exhibit a synchronous semelparity. Synchronous semelparity can create local drops in carrying capacity for the panda. We modeled the interaction of pandas and their bamboo food resources with an age structured panda population model linked to a natural history model of bamboo biomass dynamics based on literature values of bamboo biomass, and giant panda life history dynamics. This paper reports the results of our examination of the interaction between pandas and their bamboo food resource and its implications for panda conservation. In the model all panda populations were well below the carrying capacity of the habitat. The giant panda populations growth was most sensitive to changes in birth rates and removal of reproductive aged individuals. Periodic starvation that has been documented in conjunction with bamboo die-offs is probably related to the inability to move to other areas within the region where bamboo is still available. Based on the results of this model, giant panda conservation should concentrate on keeping breeding individuals in the wild, keep corridors to different bamboo species open to pandas, and to concentrate research on bamboo life history.

  11. Experimental and numerical assessment of the improvement of the load-carrying capacities of butterfly-shaped coupling components in composite structures

    International Nuclear Information System (INIS)

    Altan, Gurkan; Topcu, Muzaffer

    2010-01-01

    This study was designed to analyze the load-carrying capacities of composite structures connected face-to-face by a butterfly coupling component experimentally and numerically without adhesive. The results of the experimental studies were supported with numerical analysis. In addition, the butterfly coupling component was developed geometrically with a view to the results of the numerical and experimental studies. The change in the load-carrying capacity of the improved butterfly coupling components was analyzed numerically and experimentally to obtain new results. Half-specimens and butterfly-shaped lock components were cut with a water jet machine. Experiments and analyses were conducted to analyze the effects of coupling geometry parameters, such as the ratio of the butterfly end width to the specimen width (w/b), the ratio of the butterfly middle width to the butterfly end width (x/w), and the ratio of the butterfly half height to the specimen width (y/b). It was intended to determine the damage in the butterfly before any damage to the composite structure and to increase the service-life span of the composite structure with the repair of the butterfly lock. As a result of this study, it was determined that the geometrical fixed ratios (w/b) and (x/w) were 0.4 and 0.2 at 0.4 of (y/b) according to the experimental and numerical studies with basic and modified models

  12. Investigation on the fiber based approach to estimate the axial load carrying capacity of the circular concrete filled steel tube (CFST)

    Science.gov (United States)

    Piscesa, B.; Attard, M. M.; Suprobo, P.; Samani, A. K.

    2017-11-01

    External confining devices are often used to enhance the strength and ductility of reinforced concrete columns. Among the available external confining devices, steel tube is one of the most widely used in construction. However, steel tube has some drawbacks such as local buckling which needs to be considered when estimating the axial load carrying capacity of the concrete-filled-steel-tube (CFST) column. To tackle this problem in design, Eurocode 4 provided guidelines to estimate the effective yield strength of the steel tube material. To study the behavior of CFST column, in this paper, a non-linear analysis using a fiber-based approach was conducted. The use of the fiber-based approach allows the engineers to predict not only the axial load carrying capacity but also the complete load-deformation curve of the CFST columns for a known confining pressure. In the proposed fiber-based approach, an inverse analysis is used to estimate the constant confining pressure similar to design-oriented models. This paper also presents comparisons between the fiber-based approach model with the experimental results and the 3D non-linear finite element analysis.

  13. Human short-term spatial memory: precision predicts capacity.

    Science.gov (United States)

    Banta Lavenex, Pamela; Boujon, Valérie; Ndarugendamwo, Angélique; Lavenex, Pierre

    2015-03-01

    Here, we aimed to determine the capacity of human short-term memory for allocentric spatial information in a real-world setting. Young adults were tested on their ability to learn, on a trial-unique basis, and remember over a 1-min interval the location(s) of 1, 3, 5, or 7 illuminating pads, among 23 pads distributed in a 4m×4m arena surrounded by curtains on three sides. Participants had to walk to and touch the pads with their foot to illuminate the goal locations. In contrast to the predictions from classical slot models of working memory capacity limited to a fixed number of items, i.e., Miller's magical number 7 or Cowan's magical number 4, we found that the number of visited locations to find the goals was consistently about 1.6 times the number of goals, whereas the number of correct choices before erring and the number of errorless trials varied with memory load even when memory load was below the hypothetical memory capacity. In contrast to resource models of visual working memory, we found no evidence that memory resources were evenly distributed among unlimited numbers of items to be remembered. Instead, we found that memory for even one individual location was imprecise, and that memory performance for one location could be used to predict memory performance for multiple locations. Our findings are consistent with a theoretical model suggesting that the precision of the memory for individual locations might determine the capacity of human short-term memory for spatial information. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. A Review of Researches on Tourism Carrying Capacity Based on Meta-analysi%基于元分析的旅游承载力研究

    Institute of Scientific and Technical Information of China (English)

    张俊霞; 段文军; 赵立禄; 谢靖

    2012-01-01

    随着旅游可持续发展观念的不断深入,旅游承载力研究逐渐成为研究热点,为了了解旅游承载力当前研究现状及存在问题,并寻找其研究的新突破,文章借助元分析研究方法,对1979~2012年之间相关文献进行分类和统计。从文献数量变化、研究内容、研究对象、评价指标体系和旅游承载力评价方法等方面进行中外对比分析,分析相关研究在研究对象、指标选取和研究方法等方面的总体特征及国内外研究差异。%With the further development of the concept of the tourism sustainahle development, tourism carrying capacity is becoming a hot topic. This paper reviews the researches on tourism earrying capacity based on Mcta-analysis and compare the researches hetween China anti foreign countries. Through comparative analysis of relative literature from 1979 to 2012, we compared the umber of the literatures, contents and ohjects, evaluation index system and evaluation methodology. Then, this article analyzed the current research status and the problems in tourism carrying capacity researching. Lastly, some suggestions to deal with the existing problems were proposed.

  15. Development of human resource capacity building assistance for nuclear security

    International Nuclear Information System (INIS)

    Nakamura, Yo; Noro, Naoko

    2014-01-01

    The Integrated Support Center for Nuclear Nonproliferation and Nuclear Security (ISCN) of the Japan Atomic Energy Agency (JAEA) has been providing nuclear security human resource development projects targeting at nuclear emerging countries in Asia in cooperation with the authorities concerned including the Sandia National Laboratory (SNL) and the International Atomic Energy Agency (IAEA). In the aftermath of the attacks of Sept. 11, the threat of terrorism was internationally recognized and thus the human resource capacity building is underway as an urgent task. In order to responding to emerging threats, the human resource capacity building that ISCN has implemented thus far needs to be multilaterally analyzed in order to develop more effective training programs. This paper studies ISCN's future direction by analyzing its achievements, as well as introduces the collaborative relationships with SNL that contributes to the reflection and maintenance of international trends for the contents of nuclear security training, the nuclear security enhancement support with which Japan is to provide nuclear emerging countries in Asia, and the achievements of the nuclear security training program that ISCN implemented. (author)

  16. Antioxidant capacity of plasma after red wine intake in Human

    Directory of Open Access Journals (Sweden)

    M. Gianmmanco

    2009-01-01

    Full Text Available Aim: Antioxidant effects after consumption of red wine have been investigated in several studies but results are contradictory and the difference in the plasma antioxidant capacity (AC after intake of red wine between women and men has never been studies. This work purpose is manifold: to ascertain whether red wine intake modifies the human plasma AC; to study the behaviour of plasma AC of women in comparison with men and finally to investigate on the plasma uric acid concentration and its relationships with the plasma AC after red wine intake.

  17. Consciousness: a neural capacity for objectivity, especially pronounced in humans

    Science.gov (United States)

    Dijker, Anton J. M.

    2014-01-01

    Consciousness tends to be viewed either as subjective experience of sensations and feelings, or as perception and internal representation of objects. This paper argues that neither view sufficiently acknowledges that consciousness may refer to the brain’s most adaptive property: its capacity to produce states of objectivity. It is proposed that this capacity relies on multiple sensorimotor networks for internally representing objects and their properties in terms of expectancies, as well as on motivational and motor mechanisms involved in exploration, play, and care for vulnerable living and non-living objects. States of objectivity are associated with a very special phenomenal aspect; the experience that subjective aspects are absent and one is “just looking” at the world as it really is and can be. However, these states are normally closely preceded and followed by (and tend to be combined or fused with) sensations and feelings which are caused by activation of sensory and motivational mechanisms. A capacity for objectivity may have evolved in different species and can be conceived as a common basis for other elusive psychological properties such as intelligence, conscience, and esthetic experience; all three linked to crucial behaviors in human evolution such as tool making, cooperation, and art. The brain’s pervasive tendency to objectify may be responsible for wrongly equating consciousness with feelings and wrongly opposing it to well-learned or habitual (“unconscious”) patterns of perception and behavior. PMID:24672506

  18. Detection and measurement of antioxidant capacity in human sera

    International Nuclear Information System (INIS)

    Bognar, G.; Koeteles, G.J.; Otos, M.

    1998-01-01

    The total antioxidant capacity of human sera was measured by the Randox TAS assay and an average value of 1.55 mmol/L was found from 87 healthy adult persons. Exogenous antioxidant added to the blood could be measured additively. Upon X-irradiation of whole blood samples, the antioxidant value decreased down to 1 Gy linearly. Further decrease after higher doses, however, could not be detected. Reductions of radiation-induced human lymphocyte micronucleus frequency as a cytogenetic end-point were observed upon increasing the exogenous antioxidant level in serum with a water-soluble form of alpha-tocopherol, or a plant extract from Sylibum marianum L. in vitro. (author)

  19. Research and application advances in ecological carrying capacity%生态承载力研究和应用进展

    Institute of Scientific and Technical Information of China (English)

    向芸芸; 蒙吉军

    2012-01-01

    The study of ecological carrying capacity is an important basis for the settlement of resource shortage and environment deterioration and the realization of regional sustainable development, and also, one of the hot spots in geography, ecology, and economics. After reviewing the related research achievements, this paper elucidated the connotation of ecological carrying capacity , i. e. , a representation of the supporting function and compressive stress of complex ecosystem , introduced the universal measuring methods of ecological carrying capacity, e. g. , net primary productivity (NPP) , ecological footprint (EF) , balance of supply and demand, evaluation with comprehensive indices, and systematic modeling, and presented the research progress in the ecological carrying capacity of the regions being more concerned, including watersheds, ecological fragile areas, cities, agricultural areas, and tourism areas. The deficiencies in current studies, such as the shortage of complete scientific research system, the ambiguity of threshold value's ecological meaning, the lack of time series study, and the absence of spatial pattern analysis, were summarized, and accordingly, the development trends in action mechanisms, model construction, and practical application were prospected.%生态承载力研究是解决资源-环境矛盾、实现区域可持续发展的重要基础,也是地理学、生态学和经济学关注的热点与前沿领域.本文在综述国内外相关研究成果的基础上,阐述了生态承载力的概念内涵,即对复合生态系统支持力和压力的表征;评述了常用的研究方法,即净初级生产力估测法、生态足迹法、供需平衡法、综合指标评价法和系统模型法;介绍了目前较为关注的流域、生态脆弱区、城市、农业区和旅游区生态承载力的研究进展;总结了目前生态承载力研究存在的缺乏科学完整的研究体系、阈值的生态学指示意义不明确、动

  20. 农业生产与水资源承载力评价%Agricultural production and evaluation in terms of water resources carrying capacity

    Institute of Scientific and Technical Information of China (English)

    虞祎; 张晖; 胡浩

    2016-01-01

    AbstractBased on the evaluation of water resources carrying capacity, especially taking into account the impact of agricultural pollution on sustainable use of water resources, a comprehensive analysis was conducted on the strains of water resources due to farming and animal production in different regions of China to provide reference for rational estimation of potential agricultural growth and correct approaches for structural adjustments in agriculture. Excess nitrogen and grey water were calculated as indicators to quantify the impact of agricultural pollution on water resources. Following nutrient balance theory, excess nitrogen was the difference between the sum of nitrogen provided by chemical fertilizer, livestock manure and soil, and total nitrogen needed by farming. Grey water was the amount of water required for diluting excessively high concentration of nitrogen in water to a more environmental-friendly level. Agricultural water footprint was the sum of agricultural water and grey water used. The huge quantity of excess nitrogen produced by farming and livestock consequently led to excessive amount of grey water, which more than doubled the amount of water used in agriculture. There was therefore the need to reserve enough environmental space for diluting pollution when estimating water resources carrying capacity based on water sustainability and healthy development. Water surplus were constructed to reflect the potential of water resources to support agricultural production with detailed environmental consideration. Water surplus was the difference between water resources and agricultural water footprint. Using 2003-2012 nationwide samples, a panel data model was constructed to analyze the impact of change in sown area and livestock head on water surplus. The results suggested that the nationwide water in China could carry a maximum of 168.89 million hm2 or 3.57 billion pigs. The water resources carrying capacity model results also showed that the

  1. Current-carrying capacity dependence of composite Bi2Sr2CaCu2O8 superconductors on the liquid coolant conditions

    International Nuclear Information System (INIS)

    Romanovskii, V R; Watanabe, K; Awaji, S; Nishijima, G

    2006-01-01

    The thermal runaway conditions of the composite Bi 2 Sr 2 CaCu 2 O 8 superconductor cooled by liquid helium or liquid hydrogen are compared. The study based on the static analysis of thermoelectric modes was made when the volume fraction of the superconductor in a composite was varied. Some specific trends underlying the onset of thermal runaway in superconducting composites cooled by liquid coolants are discussed. It is stated that the operating modes of superconducting composites may be characterized by stable states during which the current-carrying capacity of a superconductor is not effectively used even with a high amount of superconductor in the composite. These states are possible due to the corresponding temperature variation of the resistivities of the matrix and the superconductor in the high operating temperature range. They have to be considered in experiments when the critical current of a superconductor is determined or when the optimal stable operating modes of the current-carrying elements based on the Bi 2 Sr 2 CaCu 2 O 8 superconductor, which is cooled by liquid coolant, are defined

  2. Sensitivity of C6 Glioma Cells Carrying the Human Poliovirus Receptor to Oncolytic Polioviruses.

    Science.gov (United States)

    Sosnovtseva, A O; Lipatova, A V; Grinenko, N F; Baklaushev, V P; Chumakov, P M; Chekhonin, V P

    2016-10-01

    A humanized line of rat C6 glioma cells expressing human poliovirus receptor was obtained and tested for the sensitivity to oncolytic effects of vaccine strains of type 1, 2, and 3 polioviruses. Presentation of the poliovirus receptor on the surface of C6 glioma cells was shown to be a necessary condition for the interaction of cells with polioviruses, but insufficient for complete poliovirus oncolysis.

  3. 环境承载力视角的浙江东极岛旅游发展研究%Study on the Tourism Development of Zhejiang Dongji Island from the Perspective of Environmental Carrying Capacity

    Institute of Scientific and Technical Information of China (English)

    徐卫星; 姜和忠; 潘惠红

    2018-01-01

    Based on the evaluation theory of resource carrying capacity,the carrying capacity of tourism resources was evaluated and analyzed from 4 aspects:the carrying capacity of space resources,the carrying capacity of natural environment,the economic carrying capacity and the social psychological bearing capacity.Based on above conclusions,Dongji Island tourism development suggestions were put forward,including the improvement of hardware construction,improving the carrying capacity of infrastructure;riching tourism projects,improving the bearing capacity of space resources;strengthening the off-season tourism development,improving the utilization intensity of tourism resources.%基于资源承载力评价理论,从空间资源承载力、自然环境承载力、经济承载力以及社会心理承载力4个方面对东极岛旅游资源承载力进行评价与分析,并基于以上分析结论提出东极岛旅游发展建议:完善硬件条件建设、提高基础设施承载力;丰富旅游项目,提高空间资源承载力;加强淡季旅游开发,提高旅游资源利用强度.

  4. Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene

    Directory of Open Access Journals (Sweden)

    Heema Hewitson

    2016-03-01

    Full Text Available The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  5. Prevalence and characterization of plasmids carrying sulfonamide resistance genes among Escherichia coli from pigs, pig carcasses and human

    OpenAIRE

    Wu, Shuyu; Dalsgaard, Anders; Hammerum, Anette M; Porsbo, Lone J; Jensen, Lars B

    2010-01-01

    Abstract Background Sulfonamide resistance is very common in Escherichia coli. The aim of this study was to characterize plasmids carrying sulfonamide resistance genes (sul1, sul2 and sul3) in E. coli isolated from pigs and humans with a specific objective to assess the genetic diversity of plasmids involved in the mobility of sul genes. Methods A total of 501 E. coli isolates from pig feces, pig carcasses and human stools were tested for their susceptibility to selected antimicrobial. Multip...

  6. Salivary pH and buffering capacity in early and late human immunodeficiency virus infection.

    Science.gov (United States)

    Hegde, Mithra N; Malhotra, Amit; Hegde, Nidarsh D

    2013-11-01

    Human immunodeficiency virus (HIV) causes severe immunosuppression due to progressive decrease in the CD4 T lymphocyte cells during the course of the disease and this affects all the body systems including glandular secretions. A number of lesions affecting the salivary glands have been noted in HIV infection. The objective of this study was to evaluate the salivary pH and the buffering capacity in HIV positive individuals and comparing it with the HIV negative healthy individuals. The study was carried out on 200 HIV positive subjects aged 20-40 years, divided into two groups on the basis of CD4 count and 100 HIV negative healthy individuals as control group. Both unstimulated and stimulated saliva were collected and the pH and buffering capacity ascertained using the saliva check kit. (GC Asia Dental Pvt. Ltd., Singapore, 508724). All the three groups were compared using the ANOVA and it was found there was highly significant decrease in pH and buffering capacity with increase in immunosuppression. The intergroup comparison was carried out using the Tukey honestly significant difference (HSD) and the Chi square test. Group 1; CD4 count 200 showed a significant decrease in unstimulated salivary flow, stimulated salivary flow, and pH in comparison to HIV negative individuals; however, change in buffering capacity in Group 2 was not significant. There is a decrease in pH and buffering capacity in HIV infected patients. This decrease may be one of the factors responsible for increased caries in HIV infected population.

  7. Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene.

    Science.gov (United States)

    Kattoor, Jobin Jose; Saurabh, Sharad; Malik, Yashpal Singh; Sircar, Shubhankar; Dhama, Kuldeep; Ghosh, Souvik; Bányai, Krisztián; Kobayashi, Nobumichi; Singh, Raj Kumar

    2017-12-01

    Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog. To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes. A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed. Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population. The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

  8. Ecological restoration and recovery in the wind-blown sand hazard areas of northern China: relationship between soil water and carrying capacity for vegetation in the Tengger Desert.

    Science.gov (United States)

    Li, XingRong; Zhang, ZhiShan; Tan, HuiJuan; Gao, YanHong; Liu, LiChao; Wang, XingPing

    2014-05-01

    The main prevention and control area for wind-blown sand hazards in northern China is about 320000 km(2) in size and includes sandlands to the east of the Helan Mountain and sandy deserts and desert-steppe transitional regions to the west of the Helan Mountain. Vegetation recovery and restoration is an important and effective approach for constraining wind-blown sand hazards in these areas. After more than 50 years of long-term ecological studies in the Shapotou region of the Tengger Desert, we found that revegetation changed the hydrological processes of the original sand dune system through the utilization and space-time redistribution of soil water. The spatiotemporal dynamics of soil water was significantly related to the dynamics of the replanted vegetation for a given regional precipitation condition. The long-term changes in hydrological processes in desert areas also drive replanted vegetation succession. The soil water carrying capacity of vegetation and the model for sand fixation by revegetation in aeolian desert areas where precipitation levels are less than 200 mm are also discussed.

  9. 1000 human genomes carry widespread signatures of GC biased gene conversion.

    Science.gov (United States)

    Dutta, Rajib; Saha-Mandal, Arnab; Cheng, Xi; Qiu, Shuhao; Serpen, Jasmine; Fedorova, Larisa; Fedorov, Alexei

    2018-04-16

    GC-Biased Gene Conversion (gBGC) is one of the important theories put forward to explain profound long-range non-randomness in nucleotide compositions along mammalian chromosomes. Nucleotide changes due to gBGC are hard to distinguish from regular mutations. Here, we present an algorithm for analysis of millions of known SNPs that detects a subset of so-called "SNP flip-over" events representing recent gBGC nucleotide changes, which occurred in previous generations via non-crossover meiotic recombination. This algorithm has been applied in a large-scale analysis of 1092 sequenced human genomes. Altogether, 56,328 regions on all autosomes have been examined, which revealed 223,955 putative gBGC cases leading to SNP flip-overs. We detected a strong bias (11.7% ± 0.2% excess) in AT- > GC over GC- > AT base pair changes within the entire set of putative gBGC cases. On average, a human gamete acquires 7 SNP flip-over events, in which one allele is replaced by its complementary allele during the process of meiotic non-crossover recombination. In each meiosis event, on average, gBGC results in replacement of 7 AT base pairs by GC base pairs, while only 6 GC pairs are replaced by AT pairs. Therefore, every human gamete is enriched by one GC pair. Happening over millions of years of evolution, this bias may be a noticeable force in changing the nucleotide composition landscape along chromosomes.

  10. Prevalence and characterization of plasmids carrying sulfonamide resistance genes among Escherichia coli from pigs, pig carcasses and human.

    Science.gov (United States)

    Wu, Shuyu; Dalsgaard, Anders; Hammerum, Anette M; Porsbo, Lone J; Jensen, Lars B

    2010-07-30

    Sulfonamide resistance is very common in Escherichia coli. The aim of this study was to characterize plasmids carrying sulfonamide resistance genes (sul1, sul2 and sul3) in E. coli isolated from pigs and humans with a specific objective to assess the genetic diversity of plasmids involved in the mobility of sul genes. A total of 501 E. coli isolates from pig feces, pig carcasses and human stools were tested for their susceptibility to selected antimicrobial. Multiplex PCR was conducted to detect the presence of three sul genes among the sulfonamide-resistant E. coli isolates. Fifty-seven sulfonamide-resistant E. coli were selected based on presence of sul resistance genes and subjected to conjugation and/or transformation experiments. S1 nuclease digestion followed by pulsed-field gel electrophoresis was used to visualize and determine the size of plasmids. Plasmids carrying sul genes were characterized by PCR-based replicon typing to allow a comparison of the types of sul genes, the reservoir and plasmid present. A total of 109/501 isolates exhibited sulfonamide resistance. The relative prevalences of sul genes from the three reservoirs (pigs, pig carcasses and humans) were 65%, 45% and 12% for sul2, sul1, and sul3, respectively. Transfer of resistance through conjugation was observed in 42/57 isolates. Resistances to streptomycin, ampicillin and trimethoprim were co-transferred in most strains. Class 1 integrons were present in 80% of sul1-carrying plasmids and 100% of sul3-carrying plasmids, but only in 5% of sul2-carrying plasmids. The sul plasmids ranged from 33 to 160-kb in size and belonged to nine different incompatibility (Inc) groups: FII, FIB, I1, FIA, B/O, FIC, N, HI1 and X1. IncFII was the dominant type in sul2-carrying plasmids (52%), while IncI1 was the most common type in sul1 and sul3-carrying plasmids (33% and 45%, respectively). Multireplicons were found associated with all three sul genes. Sul genes were distributed widely in E. coli isolated

  11. Prevalence and characterization of plasmids carrying sulfonamide resistance genes among Escherichia coli from pigs, pig carcasses and human

    Directory of Open Access Journals (Sweden)

    Hammerum Anette M

    2010-07-01

    Full Text Available Abstract Background Sulfonamide resistance is very common in Escherichia coli. The aim of this study was to characterize plasmids carrying sulfonamide resistance genes (sul1, sul2 and sul3 in E. coli isolated from pigs and humans with a specific objective to assess the genetic diversity of plasmids involved in the mobility of sul genes. Methods A total of 501 E. coli isolates from pig feces, pig carcasses and human stools were tested for their susceptibility to selected antimicrobial. Multiplex PCR was conducted to detect the presence of three sul genes among the sulfonamide-resistant E. coli isolates. Fifty-seven sulfonamide-resistant E. coli were selected based on presence of sul resistance genes and subjected to conjugation and/or transformation experiments. S1 nuclease digestion followed by pulsed-field gel electrophoresis was used to visualize and determine the size of plasmids. Plasmids carrying sul genes were characterized by PCR-based replicon typing to allow a comparison of the types of sul genes, the reservoir and plasmid present. Results A total of 109/501 isolates exhibited sulfonamide resistance. The relative prevalences of sul genes from the three reservoirs (pigs, pig carcasses and humans were 65%, 45% and 12% for sul2, sul1, and sul3, respectively. Transfer of resistance through conjugation was observed in 42/57 isolates. Resistances to streptomycin, ampicillin and trimethoprim were co-transferred in most strains. Class 1 integrons were present in 80% of sul1-carrying plasmids and 100% of sul3-carrying plasmids, but only in 5% of sul2-carrying plasmids. The sul plasmids ranged from 33 to 160-kb in size and belonged to nine different incompatibility (Inc groups: FII, FIB, I1, FIA, B/O, FIC, N, HI1 and X1. IncFII was the dominant type in sul2-carrying plasmids (52%, while IncI1 was the most common type in sul1 and sul3-carrying plasmids (33% and 45%, respectively. Multireplicons were found associated with all three sul genes

  12. Curcumin-carrying nanoparticles prevent ischemia-reperfusion injury in human renal cells.

    Science.gov (United States)

    Xu, Yong; Hu, Ning; Jiang, Wei; Yuan, Hong-Fang; Zheng, Dong-Hui

    2016-12-27

    Renal ischemia-reperfusion injury (IRI) is a major complication in clinical practice. However, despite its frequency, effective preventive/treatment strategies for this condition are scarce. Curcumin possesses antioxidant properties and is a promising potential protective agent against renal IRI, but its poor water solubility restricts its application. In this study, we constructed curcumin-carrying distearoylphosphatidylethanolamine-polyethylene glycol nanoparticles (Cur-NPs), and their effect on HK-2 cells exposed to IRI was examined in vitro. Curcumin encapsulated in NPs demonstrated improved water solubility and slowed release. Compared with the IRI and Curcumin groups, Cur-NP groups displayed significantly improved cell viability, downregulated protein expression levels of caspase-3 and Bax, upregulated expression of Bcl-2 protein, increased antioxidant superoxide dismutase level, and reduced apoptotic rate, reactive oxygen species level, and malondialdehyde content. Results clearly showed that Cur-NPs demonstrated good water solubility and slow release, as well as exerted protective effects against oxidative stress in cultured HK-2 cells exposed to IRI.

  13. Working memory capacity predicts dopamine synthesis capacity in the human striatum.

    NARCIS (Netherlands)

    Cools, R.; Gibbs, S.E.; Miyakawa, A.; Jagust, W.; D'Esposito, M.

    2008-01-01

    Evidence from psychopharmacological research has revealed that dopamine receptor agents have opposite effects on cognitive function depending on baseline levels of working memory capacity. These contrasting effects have been interpreted to reflect differential baseline levels of dopamine. Here we

  14. A study on the carrying capacity of the available habitat for the Rhinopithecus bieti population at Mt. Laojun in Yunnan, China.

    Science.gov (United States)

    Li, Li; Yu, Shixiao; Ren, Baoping; Li, Ming; Wu, Ruidong; Long, Yongcheng

    2009-06-01

    The Yunnan snub-nosed monkey is one of the most endangered primates in the world. It is experiencing a range of ongoing threats and the persisting effects of past disturbances. The prospects for this species are not very optimistic because habitat corridors are severely damaged by logging, grazing, and mining. Each group of the monkeys in different areas is facing a unique variety of threats. Based on genetic analysis, Rhinopithecus bieti should be separated into three management units for conservation, of which the Mt. Laojun management unit involves the most endangered primates. Despite the fact that the vegetation on Mt. Laojun is in a relatively pristine state, only two groups of monkeys, of a total of fewer than 300, survive in the area. With this paper, we aimed to address the capacity of the monkeys' habitat at the study site and the possible reasons for the small populations. Rapid ecological assessment based on a SPOT 5 image and field survey was used to simulate the vegetation of the whole area based on reference ecological factors of the GIS system. The vegetation map of the site was thus derived from this simulation. Based on the previous studies, the three vegetation types were identified as the suitable habitat of the monkeys. The confusion matrix-based field GPS points were applied to analyze the precision of the habitat map. Based on the map of suitable habitat of the monkeys, the utilization of the habitat and the carrying capacity were analyzed in the GIS. The confusion matrix-based field GPS points were applied to the habitat analysis process, and it was found that the habitat map was 81.3% precise. Then, with the current habitat map, we found that the mixed forest currently used by the monkeys is only a very small fraction (2.65%) of the overall potential habitat of the population, while the dark conifer forest is 4.09%. Poaching is the greatest short-term threat to this species, particularly in the southern range where local residents have a

  15. Range Analysis and Terrain Preference of Adult Southern White Rhinoceros (Ceratotherium simum in a South African Private Game Reserve: Insights into Carrying Capacity and Future Management.

    Directory of Open Access Journals (Sweden)

    S Thompson

    Full Text Available The Southern white rhinoceros (Ceratotherium simum is a threatened species, central to the tourism appeal of private game reserves in South Africa. Privately owned reserves in South Africa tend to be smaller than government run reserves such as Kruger National Park. Because of their relatively small size and the often heterogeneous nature of the landscape private game reserve managers benefit from detailed knowledge of white rhinoceros terrain selection preferences, which can be assessed from their ranging behaviours. We collected adult and sub-adult white rhinoceros distribution data over a 15 month period, calculating individual range size using kernel density estimation analysis within a GIS. From this, terrain selectivity was calculated using 50% and 95% kernels to extract terrain composition values. Jacob's correction of the Ivlev's selectivity index was subsequently applied to the terrain composition of each individual to identify trends in selectivity. Results reveal that adult males hold exclusive territories considerably smaller than those found in previous work conducted in "open" or large reserves. Similarly, results for the size of male versus female territories were also not in keeping with those from previous field studies, with males, rather than females, having the larger territory requirement. Terrain selection for both genders and age classes (adult and sub-adult showed a strong preference for open grassland and avoidance of hill slope and riparian terrains. This research reveals white rhinoceros terrain selection preferences and how they influence range requirements in small, closed reserves. We conclude that this knowledge will be valuable in future white rhinoceros conservation management in small private game reserves, particularly in decisions surrounding removal of surplus individuals or augmentation of existing populations, calculation of reserve carrying capacity and future private reserve acquisition.

  16. Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene

    Directory of Open Access Journals (Sweden)

    Heema Hewitson

    2016-03-01

    Full Text Available The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739–3742 ΔTTTG. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  17. Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

    Directory of Open Access Journals (Sweden)

    Cristian Miere

    2016-03-01

    Full Text Available The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  18. Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene

    Directory of Open Access Journals (Sweden)

    Laureen Jacquet

    2016-03-01

    Full Text Available The KCL028 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

  19. Proceedings from a Workshop on Ecological Carrying Capacity of Salmonids in the Columbia River Basin : Measure 7.1A of the Northwest Power Planning Council`s 1994 Fish and Wildlife Program : Report 3 of 4, Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, Gary E.; Neitzel, D.A.; Mavros, William V.

    1996-05-01

    This report contains the proceedings of a workshop held during 1995 in Portland, Oregon. The objective of the workshop was to assemble a group of experts that could help us define carrying capacity for Columbia River Basin salmonids. The workshop was one activity designed to answer the questions asked in Measure 7.1A of the Council`s Fish and Wildlife Program. Based, in part, on the information we learned during the workshop we concluded that the approach inherent in 7.1A will not increase understanding of ecology, carrying capacity, or limiting factors that influence salmon under current conditions. Measure 7.1A requires a definition of carrying capacity and a list of determinants (limiting factors) of capacity. The implication or inference then follows that by asking what we know and do not know about the determinants will lead to research that increases our understanding of what is limiting salmon survival. It is then assumed that research results will point to management actions that can remove or repair the limiting factors. Most ecologists and fisheries scientists that have studied carrying capacity clearly conclude that this approach is an oversimplification of complex ecological processes. To pursue the capacity parameter, that is, a single number or set of numbers that quantify how many salmon the basin or any part of the basin can support, is meaningless by itself and will not provide useful information.

  20. 我国东部沿海地区旅游环境相对承载力研究%THE STUDY ON THE RELATIVE CARRYING CAPACITY OF TOURISM ENVIRONMENT IN EASTERN COASTAL REGIONS IN CHINA

    Institute of Scientific and Technical Information of China (English)

    张广海; 刘佳

    2009-01-01

    Through evaluating on tourism environmental carrying capacity including the relative carrying capacity of tourism resources and the relative carrying capacity of tourism economy, in order to making disscussion on the sustainable development among tourism resources and tourist capacity and tourism economy, which providing a new study idea and study method for tourism environmental carrying capacity.Compared to nationwide, the whole the relative carrying capacity of tourism resources and tourism economy are extremly strong, it is in under the carrying capacitiy of tourism environment and carrying capacity potental is lalent, however, the relative carrying capacities of tourism environment in different provinces are different, regional disparities are so obvious. Therefore, the Coordinated and orderd development of tourism industry should be strengthened,in order to putting comprehensive development level of tourism industry and the whole carrying capacity of tourism envinronment.%选取旅游资源综合丰度和旅游经济指标,对旅游环境系统的承载状况和承载潜力进行定量测算,以探讨区域旅游资源开发与旅游经济发展水平之间的相互关系.研究表明,相对于全国而言,东部沿海地区整体旅游资源环境相对承载能力和旅游经济环境相对承载能力较强,处于弱载状态,具有一定的承载潜力,其中旅游环境相对承载力具有明显的地域差异,应加强各省市旅游业的协调有序发展,以提高东部沿海旅游环境整体承载力,进一步推动沿海发达地区旅游业的综合发展.

  1. Calculation and evaluation of the tourism environmental carrying capacity of Xi'an%西安市旅游环境承载力测算与评价研究

    Institute of Scientific and Technical Information of China (English)

    闫博雅; 毕超

    2011-01-01

    Aim To calculate and evaluate the tourism environmental carrying capacity of Xi'an. Methods The Calculation model of the tourism environmental carrying capacity is used. Results The tourism environmental carrying capacity of Xi'an is 282 600 passengers per day and the lower ecological carrying capacity is the primary factor that constrained tourism environmental carrying capacity in Xi'an. Conclusion Urban tourism environmental carrying capacity is about twice of the number of tourists in Xi'an in 2010, it can be improved by enhancing the city's capacity in terms of solid waste and wastewater treatment in the future.%目的 测算西安市旅游环境承栽力并对测算结果进行评价.方法 运用旅游管理学中的旅游环境承栽力测算模型进行测算.结果 计算出西安市旅游环境承载力为每日28.26万人次,并且发现生态承栽力较低是制约其旅游环境承载力的首要因素.结论 西安市旅游环境承载力约是2010年西安市日均游客接待数量的2倍,可通过改善影响旅游生态承载力的固体废弃物、废水处理能力,大幅提升西安市旅游环境承栽力.

  2. Threats to the Human Capacity of Regional Higher Education Institutions

    Directory of Open Access Journals (Sweden)

    Evgeny Valentinovich Romanov

    2018-03-01

    Full Text Available In recent years, the sphere of science and education in Russia undergoes significant reforms. However, the existing framework guiding the development of the higher education contradict the Strategy of Scientific and Technological Development of Russia. These contradictions concern the conditions for building an integral system of personnel reserve and recruitment, which is necessary for the scientific and technological development of the country. The change of the funding model and the transition to two-tier higher education contribute to the outflow of talented youth to the cities where branded universities are concentrated. It creates threats to the human capacity of regional higher education institutions (both regarding staffing number, and regarding personnel reserve. Decreasing trend in number of students because of the federal budget appropriation and the existing system of per capita funding for regional higher education institutions are the threats for regional higher education. These threats can result in permanent reduction of the number of academic teaching staff and in potential decline in quality of education due to increasing teachers’ workloads. The transition to the two-tier model of university education has changed the approach to evaluating the efficiency of scientific research. The number of publications in the journals, which are indexed in the Web of Science and Scopus, has increased, but the patent activity of the leading higher education institutions has decreased many times. The ratio of number of articles to the number of the granted patents in the leading Russian universities significantly exceeds a similar indicator of the leading foreign universities. It can be regarded as «brain drain». Furthermore, this fact explains why the specific weight of income from the results of intellectual activity in total income in the majority of the Russian universities is close to zero. Regional higher education institutions need

  3. Dogs leaving the ICU carry a very large multi-drug resistant enterococcal population with capacity for biofilm formation and horizontal gene transfer.

    Directory of Open Access Journals (Sweden)

    Anuradha Ghosh

    Full Text Available The enterococcal community from feces of seven dogs treated with antibiotics for 2-9 days in the veterinary intensive care unit (ICU was characterized. Both, culture-based approach and culture-independent 16S rDNA amplicon 454 pyrosequencing, revealed an abnormally large enterococcal community: 1.4±0.8×10(8 CFU gram(-1 of feces and 48.9±11.5% of the total 16,228 sequences, respectively. The diversity of the overall microbial community was very low which likely reflects a high selective antibiotic pressure. The enterococcal diversity based on 210 isolates was also low as represented by Enterococcus faecium (54.6% and Enterococcus faecalis (45.4%. E. faecium was frequently resistant to enrofloxacin (97.3%, ampicillin (96.5%, tetracycline (84.1%, doxycycline (60.2%, erythromycin (53.1%, gentamicin (48.7%, streptomycin (42.5%, and nitrofurantoin (26.5%. In E. faecalis, resistance was common to tetracycline (59.6%, erythromycin (56.4%, doxycycline (53.2%, and enrofloxacin (31.9%. No resistance was detected to vancomycin, tigecycline, linezolid, and quinupristin/dalfopristin in either species. Many isolates carried virulence traits including gelatinase, aggregation substance, cytolysin, and enterococcal surface protein. All E. faecalis strains were biofilm formers in vitro and this phenotype correlated with the presence of gelE and/or esp. In vitro intra-species conjugation assays demonstrated that E. faecium were capable of transferring tetracycline, doxycycline, streptomycin, gentamicin, and erythromycin resistance traits to human clinical strains. Multi-locus variable number tandem repeat analysis (MLVA and pulsed-field gel electrophoresis (PFGE of E. faecium strains showed very low genotypic diversity. Interestingly, three E. faecium clones were shared among four dogs suggesting their nosocomial origin. Furthermore, multi-locus sequence typing (MLST of nine representative MLVA types revealed that six sequence types (STs originating from five

  4. Building the Capacity to Innovate: The Role of Human Capital. Research Report

    Science.gov (United States)

    Smith, Andrew; Courvisanos, Jerry; Tuck, Jacqueline; McEachern, Steven

    2012-01-01

    This report examines the link between human resource management practices and innovation. It is based on a conceptual framework in which "human resource stimuli measures"--work organisation, working time, areas of training and creativity--feed into innovative capacity or innovation. Of course, having innovative capacity does not…

  5. Prevalence and characterization of plasmids carrying sulfonamide resistance genes among Escherichia coli from pigs, pig carcasses and human

    DEFF Research Database (Denmark)

    Shuyu, Wu; Dalsgaard, A.; Hammerum, A. M.

    2010-01-01

    isolates. Fifty-seven sulfonamide-resistant E. coli were selected based on presence of sul resistance genes and subjected to conjugation and/or transformation experiments. S1 nuclease digestion followed by pulsed-field gel electrophoresis was used to visualize and determine the size of plasmids. Plasmids...... and humans) were 65%, 45% and 12% for sul2, sul1, and sul3, respectively. Transfer of resistance through conjugation was observed in 42/57 isolates. Resistances to streptomycin, ampicillin and trimethoprim were co-transferred in most strains. Class 1 integrons were present in 80% of sul1-carrying plasmids...

  6. Reconstruction actions carried out in order to achieve nominal design heat capacity of hot water boilers type VKSM 40; A product of TPK-Zagreb

    International Nuclear Information System (INIS)

    Ninevski, Gjorgji; Sekovanikj, Ivica

    2000-01-01

    Hot water boilers with a steep turbines membranous produced by TPK-Zagreb in 1979 are projected for nominal capacity of 46.52 MW. But it was demonstrated in practice, according the performed measurements, that during the exploitation they do not give declared thermal power. In this article the chronological review of all successful performed reconstructive operations by engineering staff from Toplifikacija Joint-Stock Co. for district heating -Skopje (Macedonia) on the hot water boilers type VKSM40 with nominal capacity of 46.52 MW is given. (Authors)

  7. Human resource capacity for information management in selected ...

    African Journals Online (AJOL)

    Results: it was established that capacity building was usually undertaken through on-job trainings i.e. 85.1% (103) health workers had on-job training on filling of data collection tools and only 10% (13) had received formal classroom training on the same. Further, only 9.1% (11) health workers had received information ...

  8. Characterization of Variant Creutzfeldt-Jakob Disease Prions in Prion Protein-humanized Mice Carrying Distinct Codon 129 Genotypes*

    Science.gov (United States)

    Takeuchi, Atsuko; Kobayashi, Atsushi; Ironside, James W.; Mohri, Shirou; Kitamoto, Tetsuyuki

    2013-01-01

    To date, all clinical variant Creutzfeldt-Jakob disease (vCJD) patients are homozygous for methionine at polymorphic codon 129 (129M/M) of the prion protein (PrP) gene. However, the appearance of asymptomatic secondary vCJD infection in individuals with a PRNP codon 129 genotype other than M/M and transmission studies using animal models have raised the concern that all humans might be susceptible to vCJD prions, especially via secondary infection. To reevaluate this possibility and to analyze in detail the transmission properties of vCJD prions to transgenic animals carrying distinct codon 129 genotype, we performed intracerebral inoculation of vCJD prions to humanized knock-in mice carrying all possible codon 129 genotypes (129M/M, 129M/V, or 129V/V). All humanized knock-in mouse lines were susceptible to vCJD infection, although the attack rate gradually decreased from 129M/M to 129M/V and to 129V/V. The amount of PrP deposition including florid/amyloid plaques in the brain also gradually decreased from 129M/M to 129M/V and to 129V/V. The biochemical properties of protease-resistant abnormal PrP in the brain and transmissibility of these humanized mouse-passaged vCJD prions upon subpassage into knock-in mice expressing bovine PrP were not affected by the codon 129 genotype. These results indicate that individuals with the 129V/V genotype may be more susceptible to secondary vCJD infection than expected and may lack the neuropathological characteristics observed in vCJD patients with the 129M/M genotype. Besides the molecular typing of protease-resistant PrP in the brain, transmission studies using knock-in mice carrying bovine PrP may aid the differential diagnosis of secondary vCJD infection, especially in individuals with the 129V/V genotype. PMID:23792955

  9. Characterization of variant Creutzfeldt-Jakob disease prions in prion protein-humanized mice carrying distinct codon 129 genotypes.

    Science.gov (United States)

    Takeuchi, Atsuko; Kobayashi, Atsushi; Ironside, James W; Mohri, Shirou; Kitamoto, Tetsuyuki

    2013-07-26

    To date, all clinical variant Creutzfeldt-Jakob disease (vCJD) patients are homozygous for methionine at polymorphic codon 129 (129M/M) of the prion protein (PrP) gene. However, the appearance of asymptomatic secondary vCJD infection in individuals with a PRNP codon 129 genotype other than M/M and transmission studies using animal models have raised the concern that all humans might be susceptible to vCJD prions, especially via secondary infection. To reevaluate this possibility and to analyze in detail the transmission properties of vCJD prions to transgenic animals carrying distinct codon 129 genotype, we performed intracerebral inoculation of vCJD prions to humanized knock-in mice carrying all possible codon 129 genotypes (129M/M, 129M/V, or 129V/V). All humanized knock-in mouse lines were susceptible to vCJD infection, although the attack rate gradually decreased from 129M/M to 129M/V and to 129V/V. The amount of PrP deposition including florid/amyloid plaques in the brain also gradually decreased from 129M/M to 129M/V and to 129V/V. The biochemical properties of protease-resistant abnormal PrP in the brain and transmissibility of these humanized mouse-passaged vCJD prions upon subpassage into knock-in mice expressing bovine PrP were not affected by the codon 129 genotype. These results indicate that individuals with the 129V/V genotype may be more susceptible to secondary vCJD infection than expected and may lack the neuropathological characteristics observed in vCJD patients with the 129M/M genotype. Besides the molecular typing of protease-resistant PrP in the brain, transmission studies using knock-in mice carrying bovine PrP may aid the differential diagnosis of secondary vCJD infection, especially in individuals with the 129V/V genotype.

  10. Very low cost stand-off suicide bomber detection system using human gait analysis to screen potential bomb carrying individuals

    Science.gov (United States)

    Greneker, Gene, III

    2005-05-01

    Individuals who carry bombs on their bodies and detonate those bombs in public places are a security problem. There is belief that suicide bombings currently used in the mid-east may spread to the United States if the organized terrorist groups operating in the United States are not identified and the cell members arrested. While bombs in vehicles are the primary method currently used to spread terror in Iraq, U. S. warfighters are starting to face suicide bombers. This may become more of the situation if a stand-off detection capability is developed for the vehicle bomb case. This paper presents a concept, that if developed and commercialized, could provide an inexpensive suicide bomber screening system that could be used to screen individuals approaching a checkpoint while the individual is still 500 to 1,000 feet from the checkpoint. The proposed system measures both the radar cross-section of the individual and the radar derived gait characteristics that are associated with individuals carrying a bomb on their body. GTRI researchers propose to use human gait characteristics, as detected by radar, to determine if a human subject who is carrying no visible load on the body is actually carrying a concealed load under their clothes. The use of radar gait as a metric for the detection (as opposed to a video system) of a suicide bomber is being proposed because detection of gait characteristics are thought to be less sensitive to where the bomb is located on the body, lighting conditions, and the fact that the legs may be shrouded in a robe. The detection of a bomb using radar gait analysis may also prove to be less sensitive to changing tactics regarding where the bomb is placed on the body. An inert suicide bomb vest was constructed using water pipes to simulate the explosive devices. Wiring was added to simulated detonators. The vest weighs approximately 35 pounds. Radar data was taken on the volunteer subject wearing the vest that simulated the suicide bomb. This

  11. Some Mid-Life Ruminations on the Human Capacity to Transcend One's Acculturation.

    Science.gov (United States)

    Rousseve, Ronald J.

    1983-01-01

    The dialectical capacity of human consciousness enables us to generate alternatives in opposition to previous conditioning. Transcending one's acculturation need not leave one searching for gurus. Authentic personal meaning may be attained from an existential reawakening. (RM)

  12. Actovegin, a non-prohibited drug increases oxidative capacity in human skeletal muscle

    DEFF Research Database (Denmark)

    Søndergård, Stine D; Dela, Flemming; Helge, Jørn W

    2016-01-01

    Actovegin, a deproteinized haemodialysate of calf blood, is suggested to have ergogenic properties, but this potential effect has never been investigated in human skeletal muscle. To investigate this purported ergogenic effect, we measured the mitochondrial respiratory capacity in permeabilized h...

  13. Sampling capacity underlies individual differences in human associative learning.

    Science.gov (United States)

    Byrom, Nicola C; Murphy, Robin A

    2014-04-01

    Though much work has studied how external factors, such as stimulus properties, influence generalization of associative strength, there has been limited exploration of the influence that internal dispositions may contribute to stimulus processing. Here we report 2 studies using a modified negative patterning discrimination to test the relationship between global processing and generalization. Global processing was associated with stronger negative patterning discrimination, indicative of limited generalization between distinct stimulus compounds and their constituent elements. In Experiment 2, participants pretrained to adopt global processing similarly showed strong negative patterning discrimination. These results demonstrate considerable individual difference in capacity to engage in negative patterning discrimination and suggest that the tendency toward global processing may be one factor explaining this variability. The need for models of learning to account for this variability in learning is discussed.

  14. FDI spillovers, absorptive capacities and human capital development

    DEFF Research Database (Denmark)

    Narula, Rajneesh; Marin, Anabel

    2003-01-01

    spillovers accrue from MNE activity, and provide apreliminary understanding of why MNE spillovers remain somewhat ambiguous,particularly in developing countries, paying particular attention to human capitaldevelopment. Our analysis is supported by data from the Innovation Survey in Argentina.On the whole......It is nowadays generally accepted that inward foreign direct investment (FDI) is crucialas a source of technological spillovers. One of the objectives of this paper is to review theevidence on the quantity and quality of human capital employed by domestic and foreignfirms. We examine whether...

  15. Unto Others: Illustrating the Human Capacity for Cooperation

    Science.gov (United States)

    Morris, J. Andrew; Urbanski, John; Hunt, Jason

    2011-01-01

    Research in both evolutionary economics and evolutionary psychology provides strong evidence that human behavior can be, and is, a complex mix of hedonism and altruism with a strong inclination toward cooperation under certain conditions. In this article, behavioral assumptions made in mainstream business theory are compared and contrasted with…

  16. Erythropoietin treatment enhances muscle mitochondrial capacity in humans

    DEFF Research Database (Denmark)

    Plenge, Ulla; Belhage, Bo; Guadalupe-Grau, Amelia

    2012-01-01

    in humans. In six healthy volunteers rhEpo was administered by sub-cutaneous injection over 8 weeks with oral iron (100 mg) supplementation taken daily. Mitochondrial OXPHOS was quantified by high-resolution respirometry in saponin-permeabilized muscle fibers obtained from biopsies of the vastus lateralis...

  17. Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans

    DEFF Research Database (Denmark)

    Boushel, Robert Christopher; Gnaiger, Erich; Calbet, Jose A L

    2011-01-01

    Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely in-vivo maximal oxygen consumption (VO(2) max) is matched to state 3 muscle mitochondrial respira...

  18. Oriented immobilized anti-LDL antibody carrying poly(hydroxyethyl methacrylate) cryogel for cholesterol removal from human plasma

    International Nuclear Information System (INIS)

    Bereli, Nilay; Sener, Guelsu; Yavuz, Handan; Denizli, Adil

    2011-01-01

    Low density lipoprotein (LDL) cholesterol is a major ingredient of the plaque that collects in the coronary arteries and causes coronary heart diseases. Among the methods used for the extracorporeal elimination of LDL from intravasal volume, immunoaffinity technique using anti-LDL antibody as a ligand offers superior selectivity and specificity. Proper orientation of the immobilized antibody is the main issue in immunoaffinity techniques. In this study, anti-human β-lipoprotein antibody (anti-LDL antibody) molecules were immobilized and oriented through protein A onto poly(2-hydroxyethyl methacrylate) (PHEMA) cryogel in order to remove LDL from hypercholesterolemic human plasma. PHEMA cryogel was prepared by free radical polymerization initiated with N,N,N',N'-tetramethylene diamine (TEMED). PHEMA cryogel with a swelling degree of 8.89 g H 2 O/g and 67% macro-porosity was characterized by swelling studies, scanning electron microscope (SEM) and blood compatibility tests. All the clotting times were increased when compared with control plasma. The maximum immobilized anti-LDL antibody amount was 63.2 mg/g in the case of random antibody immobilization and 19.6 mg/g in the case of oriented antibody immobilization (protein A loading was 57.0 mg/g). Random and oriented anti-LDL antibody immobilized PHEMA cryogels adsorbed 111 and 129 mg LDL/g cryogel from hypercholesterolemic human plasma, respectively. Up to 80% of the adsorbed LDL was desorbed. The adsorption-desorption cycle was repeated 6 times using the same cryogel. There was no significant loss of LDL adsorption capacity. - Research highlights: → LDL cholesterol is a risk factor in the development of coronary heart diseases. → Antibodies against LDL are used for the selective extracorporeal removal of LDL. → Protein A is used for the oriented immobilization of anti LDL onto PHEMA cryogel. → PHEMA cryogels are biocompatible, exhibit a low pressure drop, lack diffusion resistance and viscous samples can be

  19. Oriented immobilized anti-LDL antibody carrying poly(hydroxyethyl methacrylate) cryogel for cholesterol removal from human plasma

    Energy Technology Data Exchange (ETDEWEB)

    Bereli, Nilay [Department of Chemistry, Hacettepe University, Beytepe, Ankara (Turkey); Sener, Guelsu [Nanotechnology and Nanomedicine Division, Hacettepe University, Ankara (Turkey); Yavuz, Handan, E-mail: handany@hacettepe.edu.tr [Department of Chemistry, Hacettepe University, Beytepe, Ankara (Turkey); Denizli, Adil [Department of Chemistry, Hacettepe University, Beytepe, Ankara (Turkey)

    2011-07-20

    Low density lipoprotein (LDL) cholesterol is a major ingredient of the plaque that collects in the coronary arteries and causes coronary heart diseases. Among the methods used for the extracorporeal elimination of LDL from intravasal volume, immunoaffinity technique using anti-LDL antibody as a ligand offers superior selectivity and specificity. Proper orientation of the immobilized antibody is the main issue in immunoaffinity techniques. In this study, anti-human {beta}-lipoprotein antibody (anti-LDL antibody) molecules were immobilized and oriented through protein A onto poly(2-hydroxyethyl methacrylate) (PHEMA) cryogel in order to remove LDL from hypercholesterolemic human plasma. PHEMA cryogel was prepared by free radical polymerization initiated with N,N,N',N'-tetramethylene diamine (TEMED). PHEMA cryogel with a swelling degree of 8.89 g H{sub 2}O/g and 67% macro-porosity was characterized by swelling studies, scanning electron microscope (SEM) and blood compatibility tests. All the clotting times were increased when compared with control plasma. The maximum immobilized anti-LDL antibody amount was 63.2 mg/g in the case of random antibody immobilization and 19.6 mg/g in the case of oriented antibody immobilization (protein A loading was 57.0 mg/g). Random and oriented anti-LDL antibody immobilized PHEMA cryogels adsorbed 111 and 129 mg LDL/g cryogel from hypercholesterolemic human plasma, respectively. Up to 80% of the adsorbed LDL was desorbed. The adsorption-desorption cycle was repeated 6 times using the same cryogel. There was no significant loss of LDL adsorption capacity. - Research highlights: {yields} LDL cholesterol is a risk factor in the development of coronary heart diseases. {yields} Antibodies against LDL are used for the selective extracorporeal removal of LDL. {yields} Protein A is used for the oriented immobilization of anti LDL onto PHEMA cryogel. {yields} PHEMA cryogels are biocompatible, exhibit a low pressure drop, lack diffusion

  20. Agricultural net primary production in relation to that liberated by the extinction of Pleistocene mega-herbivores: an estimate of agricultural carrying capacity?

    Energy Technology Data Exchange (ETDEWEB)

    Doughty, Christopher E; Field, Christopher B, E-mail: chris.doughty@ouce.ox.ac.uk, E-mail: cfield@ciw.edu [Department of Global Ecology, Carnegie Institution, Stanford, CA 94305 (United States)

    2010-10-15

    Mega-fauna (defined as animals > 44 kg) experienced a global extinction with 97 of 150 genera going extinct by {approx} 10 000 years ago. We estimate the net primary production (NPP) that was liberated following the global extinction of these mega-herbivores. We then explore how humans, through agriculture, gradually appropriated this liberated NPP, with specific calculations for 800, 1850, and 2000 AD. By 1850, most of the liberated NPP had been appropriated by people, but NPP was still available in the Western US, South America and Australia. NPP liberated following the extinction of the mega-herbivores was {approx} 2.5% ({approx}1.4 (between 1.2 and 1.6) Pg yr{sup -1} of 56 Pg C yr{sup -1}; Pg: petagrams) of global terrestrial NPP. Liberated NPP peaked during the onset of agriculture and was sufficient for sustaining human agriculture until {approx} 320 (250-500) years ago. Humans currently use {approx} 6 times more NPP than was utilized by the extinct Pleistocene mega-herbivores.

  1. Agricultural net primary production in relation to that liberated by the extinction of Pleistocene mega-herbivores: an estimate of agricultural carrying capacity?

    International Nuclear Information System (INIS)

    Doughty, Christopher E; Field, Christopher B

    2010-01-01

    Mega-fauna (defined as animals > 44 kg) experienced a global extinction with 97 of 150 genera going extinct by ∼ 10 000 years ago. We estimate the net primary production (NPP) that was liberated following the global extinction of these mega-herbivores. We then explore how humans, through agriculture, gradually appropriated this liberated NPP, with specific calculations for 800, 1850, and 2000 AD. By 1850, most of the liberated NPP had been appropriated by people, but NPP was still available in the Western US, South America and Australia. NPP liberated following the extinction of the mega-herbivores was ∼ 2.5% (∼1.4 (between 1.2 and 1.6) Pg yr -1 of 56 Pg C yr -1 ; Pg: petagrams) of global terrestrial NPP. Liberated NPP peaked during the onset of agriculture and was sufficient for sustaining human agriculture until ∼ 320 (250-500) years ago. Humans currently use ∼ 6 times more NPP than was utilized by the extinct Pleistocene mega-herbivores.

  2. A Study of Tourism Environmental Carrying Capacity in Yalong Bay Tropical Paradise Forest Park%亚龙湾热带天堂森林公园旅游环境容量研究

    Institute of Scientific and Technical Information of China (English)

    杨波; 王文

    2014-01-01

    选择森林公园类型旅游区---亚龙湾森林公园为研究对象,通过分析旅游容量指标体系,定量研究了该景区的旅游环境容量。研究结果表明:亚龙湾森林公园的旅游生态环境容量为6600人次,旅游空间环境容量为8244人次,旅游功能设施容量为6400人次,旅游社会心理容量为2310人次,综合旅游环境容量为6200人次。生物环境容量、交通环境容量和游客心理环境容量成为制约景区接待规模的限制因子。在三亚旅游旺季,尤其是春节黄金周期间,整个景区呈现超载状态。针对出现的过载问题,提出了新建扩建景点和游步道、扩建停车场面积、合理安排停车组织方式和园区内交通组织及建立淡旺季两套管理等措施,以提高亚龙湾森林公园的整体环境容量并缓解旅游旺季带来的环境容量超载问题。%the article selects the forest park type of tourist area---Yalong Bay T ropical Forest Park as the study object .Through the analysis of the system of tourism environmental capacity ,the article conducts a quantitative research of the tourism area's tourism environmental capacity .T he results show that the park's tourist environmental carrying capacity ,tourism spatial capacity ,tourist facilities carrying capacity ,tourist capacity of social psychology , and comprehensive tourism environment capacity are 6600 ,8244 ,6400 ,2310 and 6200 persons per day respectively . T he biological environmental capacity , traffic environmental capacity and tourists psychological environmental capacity are identified as key factors that limit the tourist carrying capacity in Yalong Bay Forest Park .In Sanya's peak tourist season in ,especially during Spring Festival ,the entire resort is in the situation of overload .To deal with the overload problem ,this article proposes several measures ,such as expanding new resort ,touring path and parking lot ,improving parking system and traffic

  3. Research on Forewarning System of Tourism Carrying Capacity in Pingtan Island Based on Fuzzy Comprehensive Evaluation%基于模糊综合评价的海岛旅游环境承载力预警研究

    Institute of Scientific and Technical Information of China (English)

    戴丽芳; 丁丽英

    2012-01-01

    以旅游环境承栽力预警相关理论为指导,采用定性和定量相结合的方法,建立旅游环境承栽力综合评价指标体系,并以平潭海岛为个案进行实证研究.结果表明:平潭旅游环境承栽力总体处于轻度超栽状态,为实现平潭旅游的可持续发展,提出了平潭旅游今后发展的重点方向.%This paper is using the process of qualitative and quantitative combination to build the tourism carrying capacity assessment criteria system under the guidance of the forewarning system of the tourism carrying capacity and based on the empirical research of Pingtan Island. The results show that. the overall tourism carrying capacity in Pingtan is slightly overloaded. In order to achieve the sustainable development of tourism in Pingtan,the paper presents key directions of its furture development.

  4. General Psychological Implications of the Human Capacity for Grief.

    Science.gov (United States)

    Brinkmann, Svend

    2018-06-01

    Much theorizing in psychology and related disciplines begins with a given model of the mind that is then applied in research projects to study concrete phenomena. Sometimes psychological research can be theory-driven in quite an explicit way, approaching the logic of the hypothetico-deductive method. Others reject this and prefer to work inductively, and, in the extreme case of positivism, perhaps try to avoid theorizing altogether. In this article I shall suggest another way to think of the relationship between psychological theories and psychological phenomena. My suggestion is not simply to replace the hypothetico-deductive model with an inductive one, but to argue that the most direct route to theories of the human mind that grasp its complexity is to begin with the Kantian question of transcendental philosophy: X exists - how is X possible? In the context of this article, I apply this questioning to the phenomenon of grief: Grief exists - what general psychological theory of the mind do we need in order to account for its possibility? I attempt to extract three general psychological points from the existence of grief, viz. (1) the deep relationality of the self, (2) the limitations of evolutionary accounts, and (3) the normativity of psychological phenomena. I shall argue that these are general psychological lessons to be learned from grief, although they could also be arrived at by considering several other significant psychological phenomena.

  5. 岩溶地区资源环境承载力分析——以贵州省为例%Research on the Resource and Environmental Carrying Capacity of Karst Region

    Institute of Scientific and Technical Information of China (English)

    王金凤; 代稳; 马士彬; 王立威

    2017-01-01

    With the rapid development of social economy, the contradiction between population, resources and environment is becoming more and more serious, and the carrying capacity of resources and environment is under great pressure, especially in karst area. Using the analytic hierarchy process and the state space method, constructing evaluation index system of resources and environment carrying capacity of the karst area from three aspects:resource carrying capacity,environmental carrying capacity and social economic coordination,selecting water resources, land resources, tourism resources, water environment, atmospheric environment, population, economy and society as the evaluation index layer,this paper will evaluate quantitatively the resources and environment carrying capacity in Guizhou Province in 2000, 2004 and 2008 and 2013 four phase. The results showed that resources and environment carrying capacity in Guizhou Province showed a low level state and have to the middle level towards the trend in 2013 because the resources and environment capacity index in 2000, 2004, 2008 and 2013 is 0.09364, 0.08957, 0.09230, 0.0113.Zunyi City, in Qiandongnan Prefecture, Guizhou southwest Prefecture three administrative region of resources and environment carrying capacity is in medium state and the rest of the six administrative regions in the lower level in nine administrative regions.%随着社会经济的飞速发展,人口、资源与环境之间的矛盾日趋加剧,资源环境承载力面临着巨大的压力,尤其是岩溶地区.本文利用层次分析与状态空间法,从资源承载力、环境承载力和社会经济协调力三个方面构建岩溶地区资源环境承载力评价体系,选取水资源、土地资源、旅游资源、水环境、大气环境、人口、经济及社会为评价指标层,对贵州省2000、2004、2008和2013年4个时相的资源环境承载力进行了定量评价.结果表明:贵州省2000、2004、2008、2013年的资源环

  6. Computing System Construction of Water Environment Carrying Capacity in Huaihe River Basin%淮河流域水环境承载能力计算系统的构建

    Institute of Scientific and Technical Information of China (English)

    严子奇; 夏军; 左其亭; 张永勇

    2009-01-01

    本文介绍了淮河流域水环境承载能力计算系统的总体设计、承载力数学模型的建立、软件系统的结构及应用.研究中通过计算在维系"良好水环境状况"目标下流域所能承载的社会经济规模上限来得到流域水环境承载能力.在耦合了社会经济系统模型、水资源转化关系模型、水环境系统模拟模型的基础上利用数据库、地理信息系统、可视化编程技术将"社会经济-水资源-水环境"系统模型、生态需水量计算模型、以及水环境承载能力计量模型中众多的边界条件、控制条件、可变参数及成果分析进行系统集成.所建立的淮河流域水环境承载能力计算系统可有效地实现流域现状水环境承裁能力的计算以及对未来不同时刻、不同频率来水条件下的承载力进行情景分析计算,为流域水环境承载能力管理提供决策支持.%In this paper, the overall designs and mathematical model of water environment carrying capacity, as well as the system structure and application of water environment carrying capacity computing system in Huaihe River Basin are presented. The carrying capacity of water environment in river basin is reached by calculating the upper limit of the socio-economic scale that watershed can carry under the "good status of water environment". The system is based on an integrated "socio-economic-water resource-water environment" model which is coupled with the socio-economic system model, the water resource transformation relation model and the simulation model of water environment system. The population size is a key indicator to reflect the water environment carrying capacity of the basin, and iterative algorithm is used to get the maximum population size of different water environment carrying capacities. This system also contains a subroutine for ecological water requirement model. The mean value of the results from the Tennant method, base flow method and

  7. Development of Spatial and Verbal Working Memory Capacity in the Human Brain

    Science.gov (United States)

    Thomason, Moriah E.; Race, Elizabeth; Burrows, Brittany; Whitfield-Gabrieli, Susan; Glover, Gary H.; Gabrieli, John D. E.

    2009-01-01

    A core aspect of working memory (WM) is the capacity to maintain goal-relevant information in mind, but little is known about how this capacity develops in the human brain. We compared brain activation, via fMRI, between children (ages 7-12 years) and adults (ages 20-29 years) performing tests of verbal and spatial WM with varying amounts (loads)…

  8. Study on carbon carried capacity in coastal city-A case study on Qingdao%沿海城市碳承载力研究——以青岛市为例

    Institute of Scientific and Technical Information of China (English)

    关丽娟; 丁德文; 刘大海; 赵鸣

    2013-01-01

    在构建沿海城市碳承载力评价指标体系的基础上,采用熵权法为指标赋权,构建了沿海城市碳承载力模型,并按照其取值范围将沿海城市碳承载水平划分为严重空载、轻度空载、适载、轻度超载和严重超载五个级别.利用所建模型和2000~ 2009年数据对青岛市碳承载状况进行了分析,研究表明:近几年来青岛市碳承载力状况良好,且2009年青岛市碳承载力为0.9左右,整体处于较为理想的适载状态.影响青岛市碳承载状况的主要因素包括经济增长、环境、能源、产业结构、城市化等方面.青岛市建立低碳发展模式降低碳排放,维持碳承载平衡应从调整产业结构、转变经济发展方式、建立低碳发展的长效机制、保护资源环境、大力开发新能源、发展低碳旅游业和进一步完善区域性碳承载力理论体系等方面努力.%The model of carbon carried capacity in the coastal city was structured on the basis of constructing the evaluation index system of carbon carried capacity in the coastal city. In this model, the weight of each index is determined by adopting the entropy weight method. According to its value scope, carbon carried capacity in the coastal city was divided into five levels: the serious no-load, mild no-load,proper loaded,mild overload and serious overload. The status of carbon carried capacity in Qingdao from 2000 to 2009 was analyzed by the above model. The results show that the carbon carried capacity state of Qingdao was good in resent years and its carbon carried capacity ratio in 2009 is 0.9 or so, which was in the ideal state. The main factors that influence Qingdao' s carbon loaded status including the economic growth, environment, energy, industry structure, urbanization, etc. are described. The low carbon development model for Qingdao should be established, for reducing the carbon emissions and maintaining the carbon loaded balance. It should endeavor to

  9. The Comprehensive Evaluation on Grassland Eco-tourism Environmental Carrying Capacity:A Case of Hulunbeier%草原生态旅游环境承载力综合评价--以呼伦贝尔为例

    Institute of Scientific and Technical Information of China (English)

    杨智勇

    2013-01-01

    生态旅游环境承载力的提出与发展,是协调景区旅游业发展与旅游地生态环境矛盾的一种有效手段和科学管理方法。依据草原生态旅游环境承载力的基本内涵,构建了由生态环境、经济环境、社会环境三个承载子系统构成的草原旅游环境承载力评价指标体系,采用层次分析法对呼伦贝尔草原2005-2012年旅游环境承载力水平进行量化测度与系统分析,指出了制约呼伦贝尔草原生态旅游环境承载力进一步发展的瓶颈因素,在此基础上提出了相关的政策建议。%With the development of the eco-tourism carrying capacity, it has become one of the effective means and scientific management methods with coordination of tourism development and tourism ecological contradiction. the paper constructs the evaluation system of grassland?eco-tourism environmental carrying capacity of three carrying subsystems including ecology, economy and society, and then uses the Analytic Hierarchy Process to quantitatively measure and systematically analyze the carrying capacity of the Grassland?eco-tourism during 2005-2012 in Hulunbeier. Finally, the paper finds out the bottle -neck factor restrained ecotourism development and then puts forward?relevant policy recommendations.

  10. Antioxidant capacity of hesperidin from citrus peel using electron spin resonance and cytotoxic activity against human carcinoma cell lines.

    Science.gov (United States)

    Al-Ashaal, Hanan A; El-Sheltawy, Shakinaz T

    2011-03-01

    Hesperidin is a flavonoid that has various pharmacological activities including anti-inflammatory, antimicrobial and antiviral activities. The aim of the study is the isolation of hesperidin from the peel of Citrus sinensis L. (Rutaceae), and the evaluation of its antioxidant capacity and cytotoxicity against different human carcinoma cell lines. In the present work, hesperidin is identified and confirmed using chromatographic and spectral analysis. To correlate between hesperidin concentration and antioxidant capacity of peel extracts, extraction was carried out using 1% HCl-MeOH, MeOH, alkaline solution, the concentration of hesperidin determined qualitatively and quantitatively using high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC) analysis, in vitro antioxidant capacity of hesperidin and the extracts against free radical diphenylpicrylhydrazyl (DPPH•) performed using an electron spin resonance spectrophotometer (ESR). Cytotoxic assay against larynx, cervix, breast and liver carcinoma cell lines was performed. Hesperidin was found to be moderately active as an antioxidant agent; its capacity reached 36%. In addition, the results revealed that hesperidin exhibited pronounced anticancer activity against the selected cell lines. IC₅₀ were 1.67, 3.33, 4.17, 4.58 µg/mL, respectively. Orange peels are considered to be a cheap source for hesperidin which may be used in the pharmaceutical industry as a natural chemopreventive agent. Hesperidin and orange peel extract could possess antioxidant properties with a wide range of therapeutic applications.

  11. Vegetation Composition, Biomass Production, Carrying Capacity ...

    African Journals Online (AJOL)

    Acacia tortilis, Acacia nilotica, Acacia mellifera and Acacia seyal were the most dominant shrubs with scattered Caddaba rotundifolia, Caddaba furmisa, Seddera bagshawei, Tamarix nilotica, Dobera glabra and abundant Parthenium hysterophorus, Cissus rotundifolia and C. quadrangularis. The grass biomass estimated in ...

  12. Residual load carrying capacity of timber joints

    NARCIS (Netherlands)

    Kuilen, J.W.G. van de

    1999-01-01

    Timber joints that have been preloaded for 2 to 8 years have been short term tested in accordance with EN 26891. The applied load levels varied between 30% and 50% of the average short term strength. The study comprised nailed, toothed-plate and split-ring joints. All joints were made of spruce and

  13. Standards for the Estimation of Tourism Carrying Capacity of Countryside Wetland Parks%郊野湿地公园旅游环境容量测算标准构建

    Institute of Scientific and Technical Information of China (English)

    张桦; 储九志

    2013-01-01

    Based on differences among geographic locations, Wetland Parks were divided into 3 types of city, suburban and countryside. A special function of the countryside wetland parks is tourism support. By reviewing the first batch of National Wetland Parks and taking into account of results from relevant studies, an indicators system for the tourism capacity of countryside wetland parks was developed. Standards for estimating the tourism carrying capacity was developed by integrating other relevant standards through questionnaires. The standards covers carrying capacities in 5 major aspects: ecology, resources, psychological, facilities and management capacity, including a total of 18 elements. The standards provided a good foundation for further studies.%基于地理位置差异将湿地公园分为城市、城郊与郊野3类,探讨郊野湿地公园承担旅游功能所具有的特殊性,通过对首批国家湿地公园的梳理,结合相关研究成果,提出郊野湿地公园旅游环境容量测算的指标体系,在此基础上采用相关标准指标,通过问卷调研等方法构建其测算标准.测算标准包括旅游环境容量中的生态、资源、心理、设施及管理5大容量,共计18项因子,为其测算研究提供良好基础.

  14. Evaluation of Marine Resource Carrying Capacity in the Construction of Qingdao Blue Economy Zone%青岛市蓝色经济区建设的海洋资源承载力评价

    Institute of Scientific and Technical Information of China (English)

    李京梅; 许玲

    2013-01-01

    From the standpoints of marine resources supply and marine industry demands ,this article built a comprehensive evaluation indicator system and measured marine resource carrying capacity in Qing-dao from 2001 to 2010 by the fuzzy comprehensive evaluation method .The results showed that the devel-opment of marine industry was beyond the marine resource carrying capacity in 2001 to 2006 and 2008 ,and was within the carrying capacity in the rest three years .The construction of the seaports has increased the supply ability of marine resources to some extent ,but the pollution caused by traditional marine industries is still huge ,and is a major cause for the bad performance of marine resource carrying capacity .It is sug-gested that traditional aquaculture should be reformed ,and environment friendly industries like recreation fishery and tourism should be developed in the process of constructing the blue economy zone .%从胶州湾海域资源环境供给和青岛市海洋产业增长需求的角度,构建了青岛市海洋资源承载力综合评价指标体系,利用模糊综合评价方法,对青岛市2001-2010年间海洋资源承载状况进行测度。结果表明,2001-2006年及2008年青岛市海洋资源承载力处于超载状态,其余年份处于适载状态。海岸带开发的港口建设在一定程度上提高了海洋资源的供给能力,但传统海洋产业的排污需求依然较大,是海洋资源承载力超载的重要原因。建议在蓝色经济区建设中,加快对传统养殖业的改造,大力发展休闲渔业、滨海旅游业等能耗低、排污少的产业。

  15. Assessing employability capacities and career adaptability in a sample of human resource professionals

    Directory of Open Access Journals (Sweden)

    Melinde Coetzee

    2015-06-01

    Full Text Available Orientation: Employers have come to recognise graduates’ employability capacities and their ability to adapt to new work demands as important human capital resources for sustaining a competitive business advantage. Research purpose: The study sought (1 to ascertain whether a significant relationship exists between a set of graduate employability capacities and a set of career adaptability capacities and (2 to identify the variables that contributed the most to this relationship. Motivation for the study: Global competitive markets and technological advances are increasingly driving the demand for graduate knowledge and skills in a wide variety of jobs. Contemporary career theory further emphasises career adaptability across the lifespan as a critical skill for career management agency. Despite the apparent importance attached to employees’ employability and career adaptability, there seems to be a general lack of research investigating the association between these constructs. Research approach, design and method: A cross-sectional, quantitative research design approach was followed. Descriptive statistics, Pearson product-moment correlations and canonical correlation analysis were performed to achieve the objective of the study. The participants (N = 196 were employed in professional positions in the human resource field and were predominantly early career black people and women. Main findings: The results indicated positive multivariate relationships between the variables and showed that lifelong learning capacities and problem solving, decision-making and interactive skills contributed the most to explaining the participants’ career confidence, career curiosity and career control. Practical/managerial implications: The study suggests that developing professional graduates’ employability capacities may strengthen their career adaptability. These capacities were shown to explain graduates’ active engagement in career management

  16. Mental time travel : A conceptual overview of social psychological perspectives on a fundamental human capacity

    NARCIS (Netherlands)

    Epstude, K.; Peetz, J.

    Humans have the unique capacity to mentally travel through time, that is, to reflect on the past, anticipate the future, and construct alternate realities in their minds. The ability to mentally travel through time affects a variety of social psychological topics. Representations of events can

  17. "Brain drain, brain gain. . . Brain sustain?" : Challenges in building Portuguese human research capacity

    NARCIS (Netherlands)

    Hasanefendic, Sandra

    2017-01-01

    This paper presents a systematic but essentially descriptive account of the policy measure of stimulating human research capacity development under the policy program "Commitment to Science" in Portugal in the period 2006-2009. It explores the conditions that contributed to the development of the

  18. Building Human Resources Management Capacity for University Research: The Case at Four Leading Vietnamese Universities

    Science.gov (United States)

    Nguyen, T. L.

    2016-01-01

    At research-intensive universities, building human resources management (HRM) capacity has become a key approach to enhancing a university's research performance. However, despite aspiring to become a research-intensive university, many teaching-intensive universities in developing countries may not have created effective research-promoted HRM…

  19. Building the Capacity to Innovate: The Role of Human Capital--Support Document

    Science.gov (United States)

    Smith, Andrew; Courvisanos, Jerry; Tuck, Jacqueline; McEachern, Steven

    2012-01-01

    This document was produced by the authors based on their research for the report "Building the Capacity to Innovate: The Role of Human Capital," and is an added resource for further information. This document contains the following appendices: (1) Survey methodology; (2) Synopsis of the literature; (3) Interview questions; and (4) Survey…

  20. Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

    NARCIS (Netherlands)

    Jeurink, P.V.; Lull Noguera, C.; Savelkoul, H.F.J.; Wichers, H.J.

    2008-01-01

    Immunomodulation by fungal compounds can be determined by the capacity of the compounds to influence the cytokine production by human peripheral blood mononuclear cells (hPBMC). These activities include mitogenicity, stimulation and activation of immune effector cells. Eight mushroom strains

  1. Gene expression demonstrates an immunological capacity of the human endolymphatic sac

    DEFF Research Database (Denmark)

    Møller, Martin Nue; Kirkeby, Svend; Vikeså, Jonas

    2015-01-01

    OBJECTIVES/HYPOTHESIS: The purpose of the present study is to explore, demonstrate, and describe the expression of genes related to the innate immune system in the human endolymphatic sac. It is hypothesized that the endolymphatic sac has a significant immunological function in the human inner ear...... was obtained. Multiple key elements of both the cellular and humoral innate immune system were expressed, including Toll-like receptors 4 and 7, as well as beta-defensin and lactoferrin. CONCLUSIONS: The present data provides the first direct evidence of an immunological capacity of the human endolymphatic sac...... immunological entity of the inner ear. LEVEL OF EVIDENCE: N/A....

  2. Comparison of extended-spectrum-β-lactamase (ESBL) carrying Escherichia coli from sewage sludge and human urinary tract infection

    International Nuclear Information System (INIS)

    Zarfel, G.; Galler, H.; Feierl, G.; Haas, D.; Kittinger, C.; Leitner, E.; Grisold, A.J.; Mascher, F.; Posch, J.; Pertschy, B.; Marth, E.; Reinthaler, F.F.

    2013-01-01

    For many years, extended-spectrum-beta-lactamase (ESBL) producing bacteria were a problem mainly located in medical facilities. Within the last decade however, ESBL-producing bacteria have started spreading into the community and the environment. In this study, ESBL-producing Escherichia coli from sewage sludge were collected, analysed and compared to ESBL-E. coli from human urinary tract infections (UTIs). The dominant ESBL-gene-family in both sample groups was bla CTX-M , which is the most prevalent ESBL-gene-family in human infection. Still, the distribution of ESBL genes and the frequency of additional antibiotic resistances differed in the two sample sets. Nevertheless, phenotyping did not divide isolates of the two sources into separate groups, suggesting similar strains in both sample sets. We speculate that an exchange is taking place between the ESBL E. coli populations in infected humans and sewage sludge, most likely by the entry of ESBL E. coli from UTIs into the sewage system. - Highlights: ► ESBL E. coli strains from sewage sludge harbour the same dominant ESBL enzymes as human isolates. ► High resistance rates for important antibiotics can be found in isolated ESBL strains. ► High phenotypic diversity of ESBL E. coli isolates from sewage sludge and from human sources. - The distribution of ESBL resistance genes in isolates from patients and environmental samples.

  3. Statistical modelling of networked human-automation performance using working memory capacity.

    Science.gov (United States)

    Ahmed, Nisar; de Visser, Ewart; Shaw, Tyler; Mohamed-Ameen, Amira; Campbell, Mark; Parasuraman, Raja

    2014-01-01

    This study examines the challenging problem of modelling the interaction between individual attentional limitations and decision-making performance in networked human-automation system tasks. Analysis of real experimental data from a task involving networked supervision of multiple unmanned aerial vehicles by human participants shows that both task load and network message quality affect performance, but that these effects are modulated by individual differences in working memory (WM) capacity. These insights were used to assess three statistical approaches for modelling and making predictions with real experimental networked supervisory performance data: classical linear regression, non-parametric Gaussian processes and probabilistic Bayesian networks. It is shown that each of these approaches can help designers of networked human-automated systems cope with various uncertainties in order to accommodate future users by linking expected operating conditions and performance from real experimental data to observable cognitive traits like WM capacity. Practitioner Summary: Working memory (WM) capacity helps account for inter-individual variability in operator performance in networked unmanned aerial vehicle supervisory tasks. This is useful for reliable performance prediction near experimental conditions via linear models; robust statistical prediction beyond experimental conditions via Gaussian process models and probabilistic inference about unknown task conditions/WM capacities via Bayesian network models.

  4. Research on tourism environmental carrying capacity of island tourist destination---A case study of Shanghai Chongming island%岛屿型旅游目的地旅游环境承载力研究--以上海崇明岛为例

    Institute of Scientific and Technical Information of China (English)

    刘世栋; 高峻

    2014-01-01

    以上海崇明岛为例,以长江隧桥贯通以及上海世博会为人类活动影响驱动因子,从时空角度选择旅游环境承载力评价模型,研究人类活动对岛屿型旅游目的地的影响以及崇明岛旅游业发展限制因子的变化。研究结果表明:崇明岛内水环境以及宾馆接待能力良好;入岛交通从以轮渡水上交通为主演变为隧桥陆上交通为主,隧桥陆上交通在“井喷式”旅游后逐步减压,但旅游旺季入岛交通限制因子也从轮渡水上交通变为隧桥陆上交通;崇明岛旅游发展的最大限制因子目前是旅游旺季的游客接待能力以及岛屿内部固体废弃物的清运能力。%Based on the spatial and temporal tourism environmental carrying capacity assessment model,taking the Yangtze River tunnel bridge linking and the EXPO as the human activity driv-ing factors,the impacts of human activity on the island-tourist destination and the limiting factors for the tourism industry development of Shanghai Chongming island are studied.The results showed that hotel reception and water carrying capacity can meet the needs of tourists;The out-land traffic had changed from ferry boat to bridge-road traffic,and the carrying capacity of road traffic gradually decompressed after “spurt tourism”,but it still was one of the limited factors in its′peak season.At last,the biggest limited factors of tourism industry were tourist reception carrying capacity in peak season and the removal capacity of solid waste for Chongming island.

  5. An experimental approach to estimation of human information processing capacity for diagnosis tasks in NPPs

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ji Tae

    2006-02-15

    The objectives of this research are 1) to determine the human's information processing capacity and 2) to describe the relationship between the information processing capacity and human factors. This research centers on the relationship, as experimentally determined, between an operator's mental workload and information flow during accident diagnosis tasks at nuclear power plants (NPPs). The relationship between the information flow rate and operator's mental workload is investigated experimentally. According to this relationship, the operator's information processing capacity can be established. Once the information processing capacity of a main control room (MCR) operator in a NPP is known, it is possible to apply it 1) to predict the operator's performance, 2) to design diagnosis tasks, and 3) to design human-machine interface. In advanced MCR, an operator's mental activity is more important than his or her physical activity. The mental workload is the portion of the operator's limited capacity that is actually required to perform a particular task. A high mental workload may cause an operator to make a mistake and consequently affect that the safe operation of NPPs. Thus, to predict an operator's performance is very important for the nuclear safety. The information processing capacity is the operator's ability to manage the amount of bits per second when an operator is diagnosing tasks or accidents. We can estimate the information processing capacity using the relationship between the information flow rate and human performance. That is, if the operator's performance decreases rapidly as the information flow rate (bit/sec) is increased, it is possible to determine the operator's information processing capacity. A diagnosis task is one of the most complex and mentally demanding tasks as well as a crucial part in maintaining the safe operation of NPPs. Diagnosis tasks refer to the overall tasks of finding the

  6. An experimental approach to estimation of human information processing capacity for diagnosis tasks in NPPs

    International Nuclear Information System (INIS)

    Kim, Ji Tae

    2006-02-01

    The objectives of this research are 1) to determine the human's information processing capacity and 2) to describe the relationship between the information processing capacity and human factors. This research centers on the relationship, as experimentally determined, between an operator's mental workload and information flow during accident diagnosis tasks at nuclear power plants (NPPs). The relationship between the information flow rate and operator's mental workload is investigated experimentally. According to this relationship, the operator's information processing capacity can be established. Once the information processing capacity of a main control room (MCR) operator in a NPP is known, it is possible to apply it 1) to predict the operator's performance, 2) to design diagnosis tasks, and 3) to design human-machine interface. In advanced MCR, an operator's mental activity is more important than his or her physical activity. The mental workload is the portion of the operator's limited capacity that is actually required to perform a particular task. A high mental workload may cause an operator to make a mistake and consequently affect that the safe operation of NPPs. Thus, to predict an operator's performance is very important for the nuclear safety. The information processing capacity is the operator's ability to manage the amount of bits per second when an operator is diagnosing tasks or accidents. We can estimate the information processing capacity using the relationship between the information flow rate and human performance. That is, if the operator's performance decreases rapidly as the information flow rate (bit/sec) is increased, it is possible to determine the operator's information processing capacity. A diagnosis task is one of the most complex and mentally demanding tasks as well as a crucial part in maintaining the safe operation of NPPs. Diagnosis tasks refer to the overall tasks of finding the root of cause of the faults or accidents. In this

  7. About rats and jackfruit trees: modeling the carrying capacity of a Brazilian Atlantic Forest spiny-rat Trinomys dimidiatus (Günther, 1877) - Rodentia, Echimyidae - population with varying jackfruit tree (Artocarpus heterophyllus L.) abundances.

    Science.gov (United States)

    Mello, J H F; Moulton, T P; Raíces, D S L; Bergallo, H G

    2015-01-01

    We carried out a six-year study aimed at evaluating if and how a Brazilian Atlantic Forest small mammal community responded to the presence of the invasive exotic species Artocarpus heterophyllus, the jackfruit tree. In the surroundings of Vila Dois Rios, Ilha Grande, RJ, 18 grids were established, 10 where the jackfruit tree was present and eight were it was absent. Previous results indicated that the composition and abundance of this small mammal community were altered by the presence and density of A. heterophyllus. One observed effect was the increased population size of the spiny-rat Trinomys dimidiatus within the grids where the jackfruit trees were present. Therefore we decided to create a mathematical model for this species, based on the Verhulst-Pearl logistic equation. Our objectives were i) to calculate the carrying capacity K based on real data of the involved species and the environment; ii) propose and evaluate a mathematical model to estimate the population size of T. dimidiatus based on the monthly seed production of jackfruit tree, Artocarpus heterophyllus and iii) determinate the minimum jackfruit tree seed production to maintain at least two T. dimidiatus individuals in one study grid. Our results indicated that the predicted values by the model for the carrying capacity K were significantly correlated with real data. The best fit was found considering 20~35% energy transfer efficiency between trophic levels. Within the scope of assumed premises, our model showed itself to be an adequate simulator for Trinomys dimidiatus populations where the invasive jackfruit tree is present.

  8. About rats and jackfruit trees: modeling the carrying capacity of a Brazilian Atlantic Forest spiny-rat Trinomys dimidiatus (Günther, 1877 – Rodentia, Echimyidae – population with varying jackfruit tree (Artocarpus heterophyllus L. abundances

    Directory of Open Access Journals (Sweden)

    JHF Mello

    Full Text Available We carried out a six-year study aimed at evaluating if and how a Brazilian Atlantic Forest small mammal community responded to the presence of the invasive exotic species Artocarpus heterophyllus, the jackfruit tree. In the surroundings of Vila Dois Rios, Ilha Grande, RJ, 18 grids were established, 10 where the jackfruit tree was present and eight were it was absent. Previous results indicated that the composition and abundance of this small mammal community were altered by the presence and density of A. heterophyllus. One observed effect was the increased population size of the spiny-rat Trinomys dimidiatus within the grids where the jackfruit trees were present. Therefore we decided to create a mathematical model for this species, based on the Verhulst-Pearl logistic equation. Our objectives were i to calculate the carrying capacity K based on real data of the involved species and the environment; ii propose and evaluate a mathematical model to estimate the population size of T. dimidiatus based on the monthly seed production of jackfruit tree, Artocarpus heterophyllus and iii determinate the minimum jackfruit tree seed production to maintain at least two T. dimidiatus individuals in one study grid. Our results indicated that the predicted values by the model for the carrying capacity K were significantly correlated with real data. The best fit was found considering 20~35% energy transfer efficiency between trophic levels. Within the scope of assumed premises, our model showed itself to be an adequate simulator for Trinomys dimidiatus populations where the invasive jackfruit tree is present.

  9. Auditory capacities in Middle Pleistocene humans from the Sierra de Atapuerca in Spain.

    Science.gov (United States)

    Martínez, I; Rosa, M; Arsuaga, J-L; Jarabo, P; Quam, R; Lorenzo, C; Gracia, A; Carretero, J-M; Bermúdez de Castro, J-M; Carbonell, E

    2004-07-06

    Human hearing differs from that of chimpanzees and most other anthropoids in maintaining a relatively high sensitivity from 2 kHz up to 4 kHz, a region that contains relevant acoustic information in spoken language. Knowledge of the auditory capacities in human fossil ancestors could greatly enhance the understanding of when this human pattern emerged during the course of our evolutionary history. Here we use a comprehensive physical model to analyze the influence of skeletal structures on the acoustic filtering of the outer and middle ears in five fossil human specimens from the Middle Pleistocene site of the Sima de los Huesos in the Sierra de Atapuerca of Spain. Our results show that the skeletal anatomy in these hominids is compatible with a human-like pattern of sound power transmission through the outer and middle ear at frequencies up to 5 kHz, suggesting that they already had auditory capacities similar to those of living humans in this frequency range.

  10. Building Innovation Capacity: The Role of Human Capital Formation in Enterprises--A Review of the Literature. Occasional Paper

    Science.gov (United States)

    Smith, Andrew; Courvisanos, Jerry; Tuck, Jacqueline; McEachern, Steven

    2011-01-01

    This literature review examines the role of human capital formation in building innovative capacity in firms. The aim of the review is to develop a model of human capital development factors to be used as a basis for a larger research project where the factors that develop innovation capacity in enterprises will be investigated. The review finds…

  11. Testing a measure of organizational learning capacity and readiness for transformational change in human services.

    Science.gov (United States)

    Bess, Kimberly D; Perkins, Douglas D; McCown, Diana L

    2011-01-01

    Transformative organizational change requires organizational learning capacity, which we define in terms of (1) internal and (2) external organizational systems alignment, and promoting a culture of learning, including (3) an emphasis on exploration and information, (4) open communication, (5) staff empowerment, and (6) support for professional development. We shortened and adapted Watkins and Marsick's Dimensions of Learning Organizations Questionnaire into a new 16-item Organizational Learning Capacity Scale (OLCS) geared more toward nonprofit organizations. The OLCS and its subscales measuring each of the above 6 dimensions are unusually reliable for their brevity. ANOVAs for the OLCS and subscales clearly and consistently confirmed extensive participant observations and other qualitative data from four nonprofit human service organizations and one local human service funding organization.

  12. Communication Capacity Research in the Majority World: Supporting the human right to communication specialist services.

    Science.gov (United States)

    Hopf, Suzanne C

    2018-02-01

    Receipt of accessible and appropriate specialist services and resources by all people with communication and/or swallowing disability is a human right; however, it is a right rarely achieved in either Minority or Majority World contexts. This paper considers communication specialists' efforts to provide sustainable services for people with communication difficulties living in Majority World countries. The commentary draws on human rights literature, particularly Article 19 of the Universal Declaration of Human Rights and the Communication Capacity Research program that includes: (1) gathering knowledge from policy and literature; (2) gathering knowledge from the community; (3) understanding speech, language and literacy use and proficiency; and (4) developing culturally and linguistically appropriate resources and assessments. To inform the development of resources and assessments that could be used by speech-language pathologists as well as other communication specialists in Fiji, the Communication Capacity Research program involved collection and analysis of data from multiple sources including 144 community members, 75 school students and their families, and 25 teachers. The Communication Capacity Research program may be applicable for achieving the development of evidence-based, culturally and linguistically sustainable SLP services in similar contexts.

  13. Maximal heart rate does not limit cardiovascular capacity in healthy humans

    DEFF Research Database (Denmark)

    Munch, G D W; Svendsen, J H; Damsgaard, R

    2014-01-01

    In humans, maximal aerobic power (VO2 max ) is associated with a plateau in cardiac output (Q), but the mechanisms regulating the interplay between maximal heart rate (HRmax) and stroke volume (SV) are unclear. To evaluate the effect of tachycardia and elevations in HRmax on cardiovascular function...... and capacity during maximal exercise in healthy humans, 12 young male cyclists performed incremental cycling and one-legged knee-extensor exercise (KEE) to exhaustion with and without right atrial pacing to increase HR. During control cycling, Q and leg blood flow increased up to 85% of maximal workload (WLmax...... and RAP (P healthy...

  14. Imaging characteristics, tissue distribution, and spread of a novel oncolytic vaccinia virus carrying the human sodium iodide symporter.

    Directory of Open Access Journals (Sweden)

    Dana Haddad

    Full Text Available INTRODUCTION: Oncolytic viruses show promise for treating cancer. However, to assess therapy and potential toxicity, a noninvasive imaging modality is needed. This study aims to determine the in vivo biodistribution, and imaging and timing characteristics of a vaccinia virus, GLV-1h153, encoding the human sodium iodide symporter (hNIS. METHODS: GLV-1h153 was modified from GLV-1h68 to encode the hNIS gene. Timing of cellular uptake of radioiodide (131I in human pancreatic carcinoma cells PANC-1 was assessed using radiouptake assays. Viral biodistribution was determined in nude mice bearing PANC-1 xenografts, and infection in tumors confirmed histologically and optically via Green Fluorescent Protein (GFP and bioluminescence. Timing characteristics of enhanced radiouptake in xenografts were assessed via (124I-positron emission tomography (PET. Detection of systemic administration of virus was investigated with both (124I-PET and 99m-technecium gamma-scintigraphy. RESULTS: GLV-1h153 successfully facilitated time-dependent intracellular uptake of (131I in PANC-1 cells with a maximum uptake at 24 hours postinfection (P<0.05. In vivo, biodistribution profiles revealed persistence of virus in tumors 5 weeks postinjection at 10(9 plaque-forming unit (PFU/gm tissue, with the virus mainly cleared from all other major organs. Tumor infection by GLV-1h153 was confirmed via optical imaging and histology. GLV-1h153 facilitated imaging virus replication in tumors via PET even at 8 hours post radiotracer injection, with a mean %ID/gm of 3.82 ± 0.46 (P<0.05 2 days after intratumoral administration of virus, confirmed via tissue radiouptake assays. One week post systemic administration, GLV-1h153-infected tumors were detected via (124I-PET and 99m-technecium-scintigraphy. CONCLUSION: GLV-1h153 is a promising oncolytic agent against pancreatic cancer with a promising biosafety profile. GLV-1h153 facilitated time-dependent hNIS-specific radiouptake in pancreatic

  15. Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1.

    Science.gov (United States)

    Allahverdi, Amir; Abroun, Saied; Jafarian, Arefeh; Soleimani, Masoud; Taghikhani, Mohammad; Eskandari, Fatemeh

    2015-01-01

    Type I diabetes is an immunologically-mediated devastation of insulin producing cells (IPCs) in the pancreatic islet. Stem cells that produce β-cells are a new promising tool. Adult stem cells such as mesenchymal stem cells (MSCs) are self renewing multi potent cells showing capabilities to differentiate into ectodermal, mesodermal and endodermal tissues. Pancreatic and duodenal homeobox factor 1 (PDX1) is a master regulator gene required for embryonic development of the pancreas and is crucial for normal pancreatic islets activities in adults. We induced the over-expression of the PDX1 gene in human bone marrow MSCs (BM-MSCs) by Lenti-PDX1 in order to generate IPCs. Next, we examine the ability of the cells by measuring insulin/c-peptide production and INSULIN and PDX1 gene expressions. After transduction, MSCs changed their morphology at day 5 and gradually differentiated into IPCs. INSULIN and PDX1 expressions were confirmed by real time polymerase chain reaction (RT-PCR) and immunostaining. IPC secreted insulin and C-peptide in the media that contained different glucose concentrations. MSCs differentiated into IPCs by genetic manipulation. Our result showed that lentiviral vectors could deliver PDX1 gene to MSCs and induce pancreatic differentiation.

  16. Demonstration of a day-night rhythm in human skeletal muscle oxidative capacity.

    Science.gov (United States)

    van Moorsel, Dirk; Hansen, Jan; Havekes, Bas; Scheer, Frank A J L; Jörgensen, Johanna A; Hoeks, Joris; Schrauwen-Hinderling, Vera B; Duez, Helene; Lefebvre, Philippe; Schaper, Nicolaas C; Hesselink, Matthijs K C; Staels, Bart; Schrauwen, Patrick

    2016-08-01

    A disturbed day-night rhythm is associated with metabolic perturbations that can lead to obesity and type 2 diabetes mellitus (T2DM). In skeletal muscle, a reduced oxidative capacity is also associated with the development of T2DM. However, whether oxidative capacity in skeletal muscle displays a day-night rhythm in humans has so far not been investigated. Lean, healthy subjects were enrolled in a standardized living protocol with regular meals, physical activity and sleep to reflect our everyday lifestyle. Mitochondrial oxidative capacity was examined in skeletal muscle biopsies taken at five time points within a 24-hour period. Core-body temperature was lower during the early night, confirming a normal day-night rhythm. Skeletal muscle oxidative capacity demonstrated a robust day-night rhythm, with a significant time effect in ADP-stimulated respiration (state 3 MO, state 3 MOG and state 3 MOGS, p < 0.05). Respiration was lowest at 1 PM and highest at 11 PM (state 3 MOGS: 80.6 ± 4.0 vs. 95.8 ± 4.7 pmol/mg/s). Interestingly, the fluctuation in mitochondrial function was also observed in whole-body energy expenditure, with peak energy expenditure at 11 PM and lowest energy expenditure at 4 AM (p < 0.001). In addition, we demonstrate rhythmicity in mRNA expression of molecular clock genes in human skeletal muscle. Our results suggest that the biological clock drives robust rhythms in human skeletal muscle oxidative metabolism. It is tempting to speculate that disruption of these rhythms contribute to the deterioration of metabolic health associated with circadian misalignment.

  17. Natural phenolic antioxidants in human fluids: analytical approaches and antioxidant capacity studies

    International Nuclear Information System (INIS)

    Zhang, K.; Zuo, Y.

    2006-01-01

    Phenolic compounds are the most abundant natural antioxidants in our diet. Epidemiological studies have shown the possible prevention effects of consumption of fruits and vegetables rich in phenolic compounds on degenerative diseases, such as cardiovascular diseases and cancers. However, there is a serious lack of fundamental knowledge on the uptake and metabolism of phenolic compounds in humans. It is clear that phenolic molecules, only absorbed by humans, can exert biological effects. This review presents a current knowledge on the analytical methods, antioxidant capacity measurements, as well as research strategies related to natural phenolic antioxidants on human health. Both GC-MS and LC-MS have proved to be very useful analytical techniques that can be employed to identify and quantitate targeted phenolic antioxidants and their metabolites in biofluids. Free radical quenching tests provide a direct measurement of antioxidant capacity but lack specificity and may oversimplify the in vivo human physiological environment. Research strategies are diverse and mainly focused on positive health effect of antioxidants. In the future studies, multiple potential bioactivities, both positive and negative, should be considered. (author)

  18. Strategies for Human Capacity Development in Nuclear Safety and Security in the NNRA

    International Nuclear Information System (INIS)

    Akpanowo, M.; Iwuala, E.

    2016-01-01

    Full text: The Nigerian Nuclear Regulatory Authority (NNRA) is charged with the responsibility for nuclear safety and radiological protection regulation. With Nigeria as one of the nuclear power states, NNRA has since 2010 intensified efforts in developing capacity to license the first set of nuclear power plants in Nigeria. The NNRA has employed several strategies in developing building and strengthening the human capacity to fulfill its statutory functions. This paper describes available human capacity development programmes and the strategies for implementing them including, knowledge retention and management strategy. The strategy commences with the recruitment process, initial training and education and continuous training. As a technical support arm of the NNRA, the National Institute for Radiation Protection and Research offers postgraduate diploma and masters programmes in radiation protection, where many NNRA officers have been trained. The NNRA also leverages on its collaboration with different international organizations for the training of its manpower in the area of nuclear safety and security. NNRA Officers participate in various IAEA organized programmes including fellowship for postgraduate diploma and master programmes, training course, workshops, seminars, conferences, scientific visits and on-the-job-training (OJT). Collaborations with other international organizations are also presented. (author

  19. Generation and Characterization of a Transgenic Mouse Carrying a Functional Human β-Globin Gene with the IVSI-6 Thalassemia Mutation

    Directory of Open Access Journals (Sweden)

    Giulia Breveglieri

    2015-01-01

    Full Text Available Mouse models that carry mutations causing thalassemia represent a suitable tool to test in vivo new mutation-specific therapeutic approaches. Transgenic mice carrying the β-globin IVSI-6 mutation (the most frequent in Middle-Eastern regions and recurrent in Italy and Greece are, at present, not available. We report the production and characterization of a transgenic mouse line (TG-β-IVSI-6 carrying the IVSI-6 thalassemia point mutation within the human β-globin gene. In the TG-β-IVSI-6 mouse (a the transgenic integration region is located in mouse chromosome 7; (b the expression of the transgene is tissue specific; (c as expected, normally spliced human β-globin mRNA is produced, giving rise to β-globin production and formation of a human-mouse tetrameric chimeric hemoglobin αmu-globin2/βhu-globin2 and, more importantly, (d the aberrant β-globin-IVSI-6 RNAs are present in blood cells. The TG-β-IVSI-6 mouse reproduces the molecular features of IVSI-6 β-thalassemia and might be used as an in vivo model to characterize the effects of antisense oligodeoxynucleotides targeting the cryptic sites responsible for the generation of aberrantly spliced β-globin RNA sequences, caused by the IVSI-6 mutation. These experiments are expected to be crucial for the development of a personalized therapy for β-thalassemia.

  20. Generation and Characterization of a Transgenic Mouse Carrying a Functional Human β-Globin Gene with the IVSI-6 Thalassemia Mutation

    Science.gov (United States)

    Mancini, Irene; Lampronti, Ilaria; Salvatori, Francesca; Fabbri, Enrica; Zuccato, Cristina; Cosenza, Lucia C.; Montagner, Giulia; Borgatti, Monica; Altruda, Fiorella; Fagoonee, Sharmila; Carandina, Gianni; Aiello, Vincenzo; Breda, Laura; Rivella, Stefano; Gambari, Roberto

    2015-01-01

    Mouse models that carry mutations causing thalassemia represent a suitable tool to test in vivo new mutation-specific therapeutic approaches. Transgenic mice carrying the β-globin IVSI-6 mutation (the most frequent in Middle-Eastern regions and recurrent in Italy and Greece) are, at present, not available. We report the production and characterization of a transgenic mouse line (TG-β-IVSI-6) carrying the IVSI-6 thalassemia point mutation within the human β-globin gene. In the TG-β-IVSI-6 mouse (a) the transgenic integration region is located in mouse chromosome 7; (b) the expression of the transgene is tissue specific; (c) as expected, normally spliced human β-globin mRNA is produced, giving rise to β-globin production and formation of a human-mouse tetrameric chimeric hemoglobin mu α-globin2/hu β-globin2 and, more importantly, (d) the aberrant β-globin-IVSI-6 RNAs are present in blood cells. The TG-β-IVSI-6 mouse reproduces the molecular features of IVSI-6 β-thalassemia and might be used as an in vivo model to characterize the effects of antisense oligodeoxynucleotides targeting the cryptic sites responsible for the generation of aberrantly spliced β-globin RNA sequences, caused by the IVSI-6 mutation. These experiments are expected to be crucial for the development of a personalized therapy for β-thalassemia. PMID:26097845

  1. Lithuanian reform on legal capacity: from soviet context towards the modern human rigths standards

    Directory of Open Access Journals (Sweden)

    Dovilė

    2015-12-01

    Full Text Available All human beings are born free and equal in dignity and rights. This is a basic fundamental principal upon which all the international law is based. Consequently people with mental disabilities too, are entitled to the enjoyment of the same human rights, in equal measure, as all other people. New international human rights treaties and documents are adopted in order to strengthen security and realisation of the rights of most vulnerable groups of people. UN Convention on the rights of persons with disabilities (CRPD is one of the newest UN’s legally binding instruments, adopted by UN General Assembley in 2006, with its purpose to “promote, protect and ensure the full and equal enjoyment of all human rights and fundamental freedoms by all persons with disabilities, and to promote respect for their inherent dignity”. The Convention bringing about a paradigm shift in attitudes of persons with disabilities as “subjects” of all human rights and basis for their protection. One of the most substantive areas that demonstrates major ‘paradigm shift’ of CRPD is provision of equality before the law to all the persons with disabilities. The right to recognition everywhere as persons before the law puts an end to various practices of the removal of rights of persons depending on their health, disability status. After the ratification of CRPD on 27 May, 2010, currently Lithuania has all legal obligations under CRPD, including the provisions on the equality before the law As in majority of other Eastern European region countries, both full guardianship and partial guardianship (curatorship meant to safeguard the human rights of vulnerable people lacking capacity existed in Lithuania for decades. Recently reform of this legal institute in order to adhere to the international human rights standards and respect the principals of disabled people human rights protection and nondiscrimination. There is no one state up to now with the developed ideal

  2. Morphological Effects and Antioxidant Capacity of Solanum crispum (Natre) In Vitro Assayed on Human Erythrocytes.

    Science.gov (United States)

    Suwalsky, Mario; Ramírez, Patricia; Avello, Marcia; Villena, Fernando; Gallardo, María José; Barriga, Andrés; Manrique-Moreno, Marcela

    2016-06-01

    In order to gain insight into the molecular mechanism of the antioxidant properties of Solanum crispum, aqueous extracts of its leaves were assayed on human erythrocytes and molecular models of its membrane. Phenolics and alkaloids were detected by HPLC-MS. Scanning electron and defocusing microscopy showed that S. crispum changed erythrocytes from the normal shape to echinocytes. These results imply that molecules present in the aqueous extracts were located in the outer monolayer of the erythrocyte membrane. Dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE) were chosen as representative of phospholipid classes located in the outer and inner monolayers of the erythrocyte membrane, respectively. X-ray diffraction showed that S. crispum preferentially interacted with DMPC bilayers. Experiments regarding its antioxidant properties showed that S. crispum neutralized the oxidative capacity of HClO on DMPE bilayers; defocusing microscopy and hemolysis assays demonstrated the protective effect of S. crispum against the oxidant effects of HClO on human erythrocytes.

  3. 基于土地消纳粪便能力的畜禽养殖承载力%Carrying capacity of livestock and poultry breeding based on feces disposal volume of land

    Institute of Scientific and Technical Information of China (English)

    潘瑜春; 孙超; 刘玉; 唐秀美; 任艳敏

    2015-01-01

    The livestock and poultry breeding industry in Pinggu district of Beijing developed rapidly. Compared with 1992, the slaughter of pigs and chickens increased by 18.95% and 22.29%, and the breeding stock of cattle, sheep, laying hens increased by 35.51%, 40.28% and 10.29%, especially the pig slaughter and egg production, which occupied by 11.81% and 20.21% of Beijing respectively. Gradually, the environmental pressure appears caused by the rapid growth of livestock and poultry manure quantity. And Pinggu district, the ecological conservation district, ecological barrier and water source protection of Beijing, is of a great importance to keep the environment of Beijing sustainable and healthy. As a result, it is important to connect farming and aquaculture, construct the circulation of agriculture economy chain, and determine the scale of farming according to local conditions. Based on the characteristic that orchard land took a large part of agricultural land, this paper set the cultivated land and orchard nitrogen limited standard, through calculation of the environmental capacity of farmland nitrogen, the poultry and animal feces pollution risk index and potential of livestock and poultry, then assessed the livestock carrying potential and potential contamination and analyzed the farmland and orchards’ livestock carrying potential of Pinggu and its town. The results indicated that: 1) During 2012, the total manure of Pinggu was 591 100 tons, containing nitrogen and phosphorus respectively 4 721, 998 t, which meant a huge organic resource, made it available to reduce the fertilizer use, and can effectively improve soil quality and the quality of the crops; 2) For ecological conservation development area, as the orchards occupied a large proportion of agricultural land, the agricultural land of livestock and poultry manure loading status achieved by taking the cultivated land and orchard as disposal site, may be more practical. The average manure load of

  4. Building capacity for human genetics and genomics research in Trinidad and Tobago

    Directory of Open Access Journals (Sweden)

    Allana Roach

    Full Text Available Advances in human genetics and genomic sciences and the corresponding explosion of biomedical technologies have deepened current understanding of human health and revolutionized medicine. In developed nations, this has led to marked improvements in disease risk stratification and diagnosis. These advances have also led to targeted intervention strategies aimed at promoting disease prevention, prolonging disease onset, and mitigating symptoms, as in the well-known case of breast cancer and the BRCA1 gene. In contrast, in the developing nation of Trinidad and Tobago, this scientific revolution has not translated into the development and application of effective genomics-based interventions for improving public health. While the reasons for this are multifactorial, the underlying basis may be rooted in the lack of pertinence of internationally driven genomics research to the local public health needs in the country, as well as a lack of relevance of internationally conducted genetics research to the genetic and environmental contexts of the population. Indeed, if Trinidad and Tobago is able to harness substantial public health benefit from genetics/genomics research, then there is a dire need, in the near future, to build local capacity for the conduct and translation of such research. Specifically, it is essential to establish a national human genetics/genomics research agenda in order to build sustainable human capacity through education and knowledge transfer and to generate public policies that will provide the basis for the creation of a mutually beneficial framework (including partnerships with more developed nations that is informed by public health needs and contextual realities of the nation.

  5. The state of human dimensions capacity for natural resource management: needs, knowledge, and resources

    Science.gov (United States)

    Sexton, Natalie R.; Leong, Kirsten M.; Milley, Brad J.; Clarke, Melinda M.; Teel, Tara L.; Chase, Mark A.; Dietsch, Alia M.

    2013-01-01

    The social sciences have become increasingly important in understanding natural resource management contexts and audiences, and are essential in design and delivery of effective and durable management strategies. Yet many agencies and organizations do not have the necessary resource management. We draw on the textbook definition of HD: how and why people value natural resources, what benefits people seek and derive from those resources, and how people affect and are affected by those resources and their management (Decker, Brown, and Seimer 2001). Clearly articulating how HD information can be used and integrated into natural resource management planning and decision-making is an important challenge faced by the HD field. To address this challenge, we formed a collaborative team to explore the issue of HD capacity-building for natural resource organizations and to advance the HD field. We define HD capacity as activities, efforts, and resources that enhance the ability of HD researchers and practitioners and natural managers and decision-makers to understand and address the social aspects of conservation.Specifically, we sought to examine current barriers to integration of HD into natural resource management, knowledge needed to improve HD capacity, and existing HD tools, resources, and training opportunities. We conducted a needs assessment of HD experts and practitioners, developed a framework for considering HD activities that can contribute both directly and indirectly throughout any phase of an adaptive management cycle, and held a workshop to review preliminary findings and gather additional input through breakout group discussions. This paper provides highlights from our collaborative initiative to help frame and inform future HD capacity-building efforts and natural resource organizations and also provides a list of existing human dimensions tools and resources.

  6. Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells

    Directory of Open Access Journals (Sweden)

    Nataša Nikolić

    2012-01-01

    Full Text Available We investigated the effects of PGC-1α (peroxisome proliferator-activated receptor γ coactivator-1α overexpression on the oxidative capacity of human skeletal muscle cells ex vivo. PGC-1α overexpression increased the oxidation rate of palmitic acid and mRNA expression of genes regulating lipid metabolism, mitochondrial biogenesis, and function in human myotubes. Basal and insulin-stimulated deoxyglucose uptake were decreased, possibly due to upregulation of PDK4 mRNA. Expression of fast fiber-type gene marker (MHCIIa was decreased. Compared to skeletal muscle in vivo, PGC-1α overexpression increased expression of several genes, which were downregulated during the process of cell isolation and culturing. In conclusion, PGC-1α overexpression increased oxidative capacity of cultured myotubes by improving lipid metabolism, increasing expression of genes involved in regulation of mitochondrial function and biogenesis, and decreasing expression of MHCIIa. These results suggest that therapies aimed at increasing PGC-1α expression may have utility in treatment of obesity and obesity-related diseases.

  7. Inhibition of Hepres virus plaquing capacity in human diploid fibroblasts treated with Gilvocarcin V plus near UV radiation

    International Nuclear Information System (INIS)

    Bockstahler, L.E.; Hitchins, V.M.; Carney, P.G.; Olvey, K.M.; Lytle, C.D.

    1990-01-01

    The capacity of human fibroblasts to support plaque formation by Herpes simplex virus following treatment of the cells with gilvocarcin V, a polyaromatic C-glycoside, plus near ultraviolet radiation (UVA, 320-400 nm) was examined. Gilvocarcin V, plus UVA radiation, effectively inhibited host cell capacity at concentrations five orders of magnitude lower than that of 8-methyoxypsoralen required for capacity inhibition at similar levels of UVA radiation. This result extends the observation of unusual biological potency of UVA-activated gilvocarcins from bacterial cells to human cells. (author)

  8. Topographic variation in redifferentiation capacity of chondrocytes in the adult human knee joint.

    Science.gov (United States)

    Stenhamre, H; Slynarski, K; Petrén, C; Tallheden, T; Lindahl, A

    2008-11-01

    The aim of this study was to investigate the topographic variation in matrix production and cell density in the adult human knee joint. Additionally, we have examined the redifferentiation potential of chondrocytes expanded in vitro from the different locations. Full thickness cartilage-bone biopsies were harvested from seven separate anatomical locations of healthy knee joints from deceased adult human donors. Chondrocytes were isolated, expanded in vitro and redifferentiated in a pellet mass culture. Biochemical analysis of total collagen, proteoglycans and cellular content as well as histology and immunohistochemistry were performed on biopsies and pellets. In the biochemical analysis of the biopsies, we found lower proteoglycan to collagen (GAG/HP) ratio in the non-weight bearing (NWB) areas compared to the weight bearing (WB) areas. The chondrocytes harvested from different locations in femur showed a significantly better attachment and proliferation ability as well as good post-expansion chondrogenic capacity in pellet mass culture compared with the cells harvested from tibia. These results demonstrate that there are differences in extra cellular content within the adult human knee in respect to GAG/HP ratio. Additionally, the data show that clear differences between chondrocytes harvested from femur and tibia from healthy human knee joints exist and that the differences are not completely abolished during the process of de- and redifferentiation. These findings emphasize the importance of the understanding of topographic variation in articular cartilage biology when approaching new cartilage repair strategies.

  9. 襄阳市长寿岛国家湿地公园生态旅游环境承载力分析%Eco-tourism Environmental Carrying Capacity of the Changshou Island National Wetland Park in Xiangyang City

    Institute of Scientific and Technical Information of China (English)

    冯芳; 张弢; 李柱

    2015-01-01

    Eco-tourism environmental capacity(ETEC) is the important evaluation basis of the harmonious relation between tourism activities and eco-environment resources. Wetland parks construction has been as the effective method for protecting and exploiting the wetland resources. ETEC assessment can effectively promote the sustainable development of wetland ecotourism. Taking the Changshou Island National Wetland Park in Xiangyang City as an example, this paper scientific calculated the Eco-tourism carrying capacity and discussed the features, evaluation indicators selection and potential risks of the ETEC. According to the limitation factors, this paper has put forward the corresponding optimal projects. The results of this study offer a scientific decision-making for the programming and management of wetland tourism and promote realizing the sustainable development of the wetland park.%旅游环境承载力是判断旅游活动与生态环境是否协调的重要指标. 建设湿地公园是湿地保护和开发的重要途径,评估湿地公园生态旅游环境承载力能有效促进其可持续发展. 文章以襄阳市长寿岛国家湿地公园作为研究对象,从旅游环境承载力的特征、指标、潜在问题等,尝试对它的生态旅游综合环境承载力进行了评估. 并依据环境承载力的限制因子,结合湿地实际情况提出了相应的优化策略,以期为湿地旅游资源开发的长期规划提供客观依据,为实现湿地公园的可持续发展提供必要的参考.

  10. Generation of human iPSC line from a patient with laterality defects and associated congenital heart anomalies carrying a DAND5 missense alteration

    Directory of Open Access Journals (Sweden)

    Fernando Cristo

    2017-12-01

    Full Text Available A human iPSC line was generated from exfoliated renal epithelial (ERE cells of a patient affected with Congenital Heart Disease (CHD and Laterality Defects carrying tshe variant p.R152H in the DAND5 gene. The transgene-free iPSCs were generated with the human OSKM transcription factor using the Sendai-virus reprogramming system. The established iPSC line had the specific heterozygous alteration, a stable karyotype, expressed pluripotency markers and generated embryoid bodies that can differentiate towards the three germ layers in vitro. This iPSC line offers a useful resource to study the molecular mechanisms of cardiomyocyte proliferation, as well as for drug testing.

  11. Capacity of ultraviolet-induced DNA repair in human glioma cells

    Energy Technology Data Exchange (ETDEWEB)

    Itoh, Hiroji

    1987-04-01

    A DNA repair abnormality is likely related to an increased incidence of neoplasms in several autosomal recessive diseases such as xeroderma pigmentosum, Fanconi's anemia, Bloom's syndrome and ataxia telangiectasia. In human glioma cells, however, there are only a few reports on DNA repair. In this study, an ultraviolet (UV)-induced DNA repair was examined systematically in many human glioma cells. Two human malignant glioma cell lines (MMG-851, U-251-MG) and 7 human glioma cell strains (4, benign; 3, malignant) of short term culture, in which glial fibrillary acidic protein (GFAP) staining were positive, were used. To investigate the capacity of DNA repair, UV sensitivity was determined by colony formation; excision repair by autoradiography and Cytosine Arabinoside (Ara-C) assay; and post-replication repair by the joining rate of newly synthesized DNA. As a result, the colony-forming abilities of malignant glioma cell lines were lower than those of normal human fibroblasts, but no difference was found between two malignant glioma cell lines. The excision repair of the malignant group (2 cell lines and 3 cell strains) was apparently lower than that of the benign group (4 cell strains). In two malignant glioma cell lines, the excision repair of MMG-851 was lower than that of U-251-MG, and the post-replication repair of MMG-851 was higher than that of U-251-MG. These results were considered to correspond well with colony-forming ability. The results indicate that there are some differences in each human malignant glioma cell in its UV-induced DNA repair mechanism, and that the excision repair of the malignant glioma cells is apparently lower than that of the benign glioma cells. These findings may be useful for diagnosis and treatment.

  12. 旅游者数量与旅游环境承载力耦合关系研究%Coupling Relationships Research of Tourist Number and Tourism Environment Carrying Capacity

    Institute of Scientific and Technical Information of China (English)

    杨秀平; 王立岩; 翁钢民

    2015-01-01

    The realities of rapid development of China′s tourism industry , the rapid expansion of tourism demand , con-centration of tourist holiday travel and the uneven spatial distribution , result in problems that negative impact of tourism activities on the environment has become increasingly prominent .Using the coupling theory , and taking the sustainable carrying of tourism environment as the objective , this paper defines the connotation of coupling relationship about tourist number and tourism environment carrying capacity , and analyzes the characteristics and application mechanism , forma-tion mechanism and the module of coupling relationship , as well as takes Nandaihe International Amusement Centre spot as an example to analyze .The research shows that the development of the tourism environment in the system depends on the matching degree between the elements , so we should refine the interaction between the tourism and the tourism envi-ronment to make the mechanism and process of coupling concrete .%旅游业的快速发展、 旅游需求的激增、 国内假期旅游的集中及旅游资源空间分布的不均,导致旅游环境承载力与旅游者人数之间的矛盾日益突出. 本文借鉴耦合理论, 以旅游环境可持续承载为目标, 界定了旅游者数量与旅游环境承载力耦合关系的内涵, 分析了该耦合关系的特征与适用机制、 形成机理与模块组成, 并以河北省南戴河国际娱乐中心旅游区为例进行了实证分析. 研究表明: 系统内旅游环境发展状况取决于构成要素之间的匹配程度, 需细化旅游者与旅游环境承载力耦合的相互作用, 使耦合的机理和流程具体化.

  13. Vulnerabilidad sísmica y capacidad de carga de un puente en acero basado en confiabilidad estructural Seismic vulnerability and load carrying capacity studies of a steel bridge based on structural relia

    Directory of Open Access Journals (Sweden)

    Edgar Muñoz

    2008-01-01

    Full Text Available Por medio de la confiabilidad estructural, para un Puente en Colombia después de su monitoreo. Los resultados de este trabajo son parte de una nueva metodología para evaluar puentes existentes mediante una colaboración entre Universidad-Empresas-Gobierno Nacional. Este estudio ayuda en la toma de decisiones y en la priorización de tareas de rehabilitación. Este estudio incluyó: caracterización dinámica de las vibraciones naturales del puente; adquisición de datos de fuerzas internas debidas al tráfico por medio de tecnología de LVDT's y Strain Gages; evaluación de las fuerzas actuales comparadas contra los esfuerzos máximos permitidos; modelación estática, dinámica y estructural. Adicionalmente se hizo un estudio de amenaza sísmica y respuesta dinámica del área del puente y nueve (9 diferentes espectros de respuesta fueron obtenidos, cada uno con un periodo de retorno diferente. Se encontró que las torres del puente tienen una probabilidad de falla mayor que la permitida por los códigos. Lo anterior condujo a un reforzamiento inmediato del puente.In this document, seismic vulnerability and load carrying capacity analyses are presented for a bridge in Colombia after monitoring by means of reliability of structures. Results of this work are part of a new methodology to evaluate existing bridges carried out though a partnership among University - Private Companies - and Goverment. This study helps in decision making on the priority of rehabilitation tasks. This study included: dynamic characterization of natural vibrations of the bridge; data logging of inner forces due to traffic by using LVDT's and strain gages technology; evaluation of actual forces to be compared to allowed stresses, dynamic and static structural modelling. Also, a seismic hazard and dynamic response of the local area of the bridge was developed and nine (9 different response spectra were obtained, each one varying its return period. It was found that the

  14. Storage at -80°C preserves the antioxidant capacity of preterm human milk.

    Science.gov (United States)

    Akdag, Arzu; Sari, Fatma Nur; Dizdar, Evrim Alyamac; Uras, Nurdan; Isikoglu, Semra; Erel, Ozcan; Dilmen, Ugur

    2014-09-01

    It is essential to establish optimum parameters for maintaining the quality of stored milk until the moment of consumption with minimal deterioration of its properties. The aim of the study was to evaluate total antioxidant capacity (TAC) and total oxidation status (TOS) of fresh and freeze-stored samples (at -80°C) of preterm human milk (HM). Samples of colostrum were collected from 98 healthy women within the first 4 days after delivery. The total milk volume collected (6 ml) was divided in two aliquot parts: 3 ml for the fresh analysis which was done immediately after the extraction and 3 ml for storage under freezing conditions at -80°C for three months. The antioxidant status and oxidative stress of the fresh and stored breast milk were assessed via determination of TAC and TOS levels. The mean gestational age and the birth weight of the infants were 31.26 ± 2.93 weeks and 1620 ± 581.91 g; respectively. There were no significant correlations between maternal age, route of delivery and milk oxidative stress. There was no significant difference between the levels of TAC, TOS and the oxidative stress index in fresh and freeze-stored samples of colostrum in preterm HM (p > 0.05). Freeze storage of preterm HM at -80°C for three months preserves the antioxidant capacity without changing oxidative status of HM, which could be noteworthy for the preterm infant nutrition. © 2014 Wiley Periodicals, Inc.

  15. Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro

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    Shingo Kanao

    2017-01-01

    Full Text Available The dental follicle is an ectomesenchymal tissue surrounding the developing tooth germ. Human dental follicle cells (hDFCs have the capacity to commit to differentiation into multiple cell types. Here we investigated the capacity of hDFCs to differentiate into neural cells and the efficiency of a two-step strategy involving floating neurosphere-like bodies for neural differentiation. Undifferentiated hDFCs showed a spindle-like morphology and were positive for neural markers such as nestin, β-III-tubulin, and S100β. The cellular morphology of several cells was neuronal-like including branched dendrite-like processes and neurites. Next, hDFCs were used for neurosphere formation in serum-free medium containing basic fibroblast growth factor, epidermal growth factor, and B27 supplement. The number of cells with neuronal-like morphology and that were strongly positive for neural markers increased with sphere formation. Gene expression of neural markers also increased in hDFCs with sphere formation. Next, gene expression of neural markers was examined in hDFCs during neuronal differentiation after sphere formation. Expression of Musashi-1 and Musashi-2, MAP2, GFAP, MBP, and SOX10 was upregulated in hDFCs undergoing neuronal differentiation via neurospheres, whereas expression of nestin and β-III-tubulin was downregulated. In conclusion, hDFCs may be another optimal source of neural/glial cells for cell-based therapies to treat neurological diseases.

  16. Variability in DNA repair capacity in the human population and its relationship to carcinogenic risk

    International Nuclear Information System (INIS)

    Nuzzo, F.; Stefanini, M.; Giulotto, E.; Falaschi, A.

    1980-01-01

    Several inherited diseases, all characterized by a high incidence of tumours in the homozygous patients, show pronounced defects in DNA repair mechanisms, thus confirming the relationship between the repair process and mutation induction, and indicating clearly that a fraction of the population is certainly much more exposed to cancer that the bulk of the human population. The basic molecular defects in such diseases are summarized. The estimated heterozygote frequency in tumour predisposing syndromes is considered and possible identification of heterozygotes discussed. A procedure to reveal DNA repair capacity at the cellular level would perhaps identify the cancer-prone fraction of the population. A simple assay for measuring repair synthesis is outlined which can be used to determine whether a given substance or treatment elicits repair synthesis and is hence harmful to DNA and potentially mutagenic and/or carcinogenic. It can also be used to assess the capacity of an individual to respond to a known DNA damaging agent. (Auth./C.F.)

  17. International Conference on Human Resource Development for Nuclear Power Programmes: Building and Sustaining Capacity. Presentations

    International Nuclear Information System (INIS)

    2014-01-01

    The objectives of the conference are to: • Review developments in the global status of HRD since the 2010 international conference; • Emphasize the role of human resources and capacity building programmes at the national and organizational level for achieving safe, secure and sustainable nuclear power programmes; • Discuss the importance of building competence in nuclear safety and security; • Provide a forum for information exchange on national, as well as international, policies and practices; • Share key elements and best practices related to the experience of Member States that are introducing, operating or expanding nuclear power programmes; • Highlight the practices and issues regarding HRD at the organizational and national level; • Highlight education and training programmes and practices; • Emphasize the role of nuclear knowledge management for knowledge transfer and HRD; and • Elaborate on the role and scope of various knowledge networks

  18. Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers

    Directory of Open Access Journals (Sweden)

    Gabriele A. Losa

    1998-01-01

    Full Text Available The proliferative capacity (%S‐phase fraction, DNA ploidy, apoptosis frequency (DNA fragmentation and steroid hormone receptor status (estrogen receptor, ER; progesterone receptor, PR of 110 samples of human breast tissues with ductal invasive carcinoma were measured using biochemical and cytofluorimetric procedures. The DNA fragmentation had a left‐skewed frequency distribution and an overall median value of 1.64%, whilst the median %S‐phase fraction was 8%. The median %DNA fragmentation and %S‐phase fraction were 1.96% and 16% in hyperdiploid tumours (n=29; DNA index >1.1 higher than in hypodiploid tumors (n=10; DNA index 0.96, 0.38% and 7.5%. DNA diploid tumours (n=71 had median %DNA fragmentation and %S‐phase values of 1.68% and 6%, consistently lower than the median values of DNA hyperdiploid tumours. The ER content of hypodiploid tumours was about one half (median: 5.9 fmol/mg the median values in hyperdiploid (10.6 fmol/mg and diploid tumours (14.6 fmol/mg. This may correlate with the lowest frequency of apoptosis in hypodiploid tumours, at least when measured by biochemical methods which only detect cells in the late phases of apoptosis. In contrast, the median PR was lowest in hyperdiploid tumours than in hypo and/or diploid tumours. The %S‐phase/%fragmented DNA ratio for the hypodiploid tumours was 19.7, significantly higher than the ratios for hyperdiploid (8.2 and diploid tumours (3.6. These findings indicated that there is an imbalance between proliferative capacity and cell death or growth arrest in human breast tumours. This imbalance may well be linked to a loss of steroid hormone control.

  19. Impact of human platelet lysate on the expansion and chondrogenic capacity of cultured human chondrocytes for cartilage cell therapy.

    Science.gov (United States)

    Sykes, J G; Kuiper, J H; Richardson, J B; Roberts, S; Wright, K T; Kuiper, N J

    2018-05-01

    High hopes have been pinned on regenerative medicine strategies in order to prevent the progression of cartilage damage to osteoarthritis, particularly by autologous chondrocyte implantation (ACI). The loss of chondrocyte phenotype during in vitro monolayer expansion, a necessary step to obtain sufficient cell numbers, may be a key limitation in ACI. In this study, it was determined whether a shorter monolayer expansion approach could improve chondrogenic differentiation. The effects of two supplement types, foetal bovine serum (FBS) and Stemulate™ (a commercial source of human platelet lysate), on the expansion and re-differentiation potential of human chondrocytes, isolated from five individuals, were compared. Chondrocytes were expanded with 10 % FBS or 10 % Stemulate™. Pellets were cultured for 28 d in chondrogenic differentiation medium and assessed for the presence of cartilage matrix molecules and genes associated with chondrogenicity. Stemulate™ significantly enhanced the proliferation rate [average population doubling times: FBS, 25.07 ± 6.98 d (standard error of the mean, SEM) vs. Stemulate™, 13.10 ± 2.57 d (SEM)]. Sulphated glycosaminoglycans (sGAG), total collagen and qRT-PCR analyses of cartilage genes showed that FBS-expanded chondrocytes demonstrated significantly better chondrogenic capacity than Stemulate™-expanded chondrocytes. Histologically, FBS-expanded chondrocyte pellets appeared to be more stable, with a more intense staining for toluidine blue, indicating a greater chondrogenic capacity. Although Stemulate™ positively influenced chondrocyte proliferation, it had a negative effect on chondrogenic differentiation potential. This suggested that, in the treatment of cartilage defects, Stemulate™ might not be the ideal supplement for expanding chondrocytes (which maintained a chondrocyte phenotype) and, hence, for cell therapies (including ACI).

  20. Impact of human platelet lysate on the expansion and chondrogenic capacity of cultured human chondrocytes for cartilage cell therapy

    Directory of Open Access Journals (Sweden)

    JG Sykes

    2018-05-01

    Full Text Available High hopes have been pinned on regenerative medicine strategies in order to prevent the progression of cartilage damage to osteoarthritis, particularly by autologous chondrocyte implantation (ACI. The loss of chondrocyte phenotype during in vitro monolayer expansion, a necessary step to obtain sufficient cell numbers, may be a key limitation in ACI. In this study, it was determined whether a shorter monolayer expansion approach could improve chondrogenic differentiation. The effects of two supplement types, foetal bovine serum (FBS and Stemulate™ (a commercial source of human platelet lysate, on the expansion and re-differentiation potential of human chondrocytes, isolated from five individuals, were compared. Chondrocytes were expanded with 10 % FBS or 10 % Stemulate™. Pellets were cultured for 28 d in chondrogenic differentiation medium and assessed for the presence of cartilage matrix molecules and genes associated with chondrogenicity. Stemulate™ significantly enhanced the proliferation rate [average population doubling times: FBS, 25.07 ± 6.98 d (standard error of the mean, SEM vs. Stemulate™, 13.10 ± 2.57 d (SEM]. Sulphated glycosaminoglycans (sGAG, total collagen and qRT-PCR analyses of cartilage genes showed that FBS-expanded chondrocytes demonstrated significantly better chondrogenic capacity than Stemulate™-expanded chondrocytes. Histologically, FBS-expanded chondrocyte pellets appeared to be more stable, with a more intense staining for toluidine blue, indicating a greater chondrogenic capacity. Although Stemulate™ positively influenced chondrocyte proliferation, it had a negative effect on chondrogenic differentiation potential. This suggested that, in the treatment of cartilage defects, Stemulate™ might not be the ideal supplement for expanding chondrocytes (which maintained a chondrocyte phenotype and, hence, for cell therapies (including ACI.

  1. Diffuse glomerular nodular lesions in diabetic pigs carrying a dominant-negative mutant hepatocyte nuclear factor 1-alpha, an inheritant diabetic gene in humans.

    Directory of Open Access Journals (Sweden)

    Satoshi Hara

    Full Text Available Glomerular nodular lesions, known as Kimmelstiel-Wilson nodules, are a pathological hallmark of progressive human diabetic nephropathy. We have induced severe diabetes in pigs carrying a dominant-negative mutant hepatocyte nuclear factor 1-alpha (HNF1α P291fsinsC, a maturity-onset diabetes of the young type-3 (MODY3 gene in humans. In this model, glomerular pathology revealed that formation of diffuse glomerular nodules commenced as young as 1 month of age and increased in size and incidence until the age of 10 months, the end of the study period. Immunohistochemistry showed that the nodules consisted of various collagen types (I, III, IV, V and VI with advanced glycation end-product (AGE and Nε-carboxymethyl-lysine (CML deposition, similar to those in human diabetic nodules, except for collagen type I. Transforming growth factor-beta (TGF-β was also expressed exclusively in the nodules. The ultrastructure of the nodules comprised predominant interstitial-type collagen deposition arising from the mesangial matrices. Curiously, these nodules were found predominantly in the deep cortex. However, diabetic pigs failed to show any of the features characteristic of human diabetic nephropathy; e.g., proteinuria, glomerular basement membrane thickening, exudative lesions, mesangiolysis, tubular atrophy, interstitial fibrosis, and vascular hyalinosis. The pigs showed only Armanni-Ebstein lesions, a characteristic tubular manifestation in human diabetes. RT-PCR analysis showed that glomeruli in wild-type pigs did not express endogenous HNF1α and HNF1β, indicating that mutant HNF1α did not directly contribute to glomerular nodular formation in diabetic pigs. In conclusion, pigs harboring the dominant-negative mutant human MODY3 gene showed reproducible and distinct glomerular nodules, possibly due to AGE- and CML-based collagen accumulation. Although the pathology differed in several respects from that of human glomerular nodular lesions, the

  2. BALANCE CAPACITY WITH VARIABILITY CAUSED BY HUMAN FACTOR: AN APPLICATION IN A LINE WITH MONTE CARLO SIMULATION

    Directory of Open Access Journals (Sweden)

    Augusto Sandes Mendes

    2016-07-01

    Full Text Available The variation in processing times due to the human factor between the working stations of a production line can generate queues resulting in a higher cost to the productive process. The objective of this study was to apply Monte Carlo simulation to balance the capacity of a production line with stations suffering variability in processing time due to the human factor. Simulations of the current situation of the production line were performed by comparing it with a proposal to align capacity with the production restriction in order to reduce inventories process while maintaining full capacity of the line. To develop the study, the selection involves the case of a company’s production line in the metal industry that produces machinery and products for metalworking area. The results allowed pointing suggestions for the company to reduce in-process inventory, keeping then total capacity of the line.

  3. Enhanced capacity of DNA repair in human cytomegalovirus-infected cells

    International Nuclear Information System (INIS)

    Nishiyama, Y.; Rapp, F.

    1981-01-01

    Plaque formation in Vero cells by UV-irradiated herpes simplex virus was enhanced by infection with human cytomegalovirus (HCMV), UV irradiation, or treatment with methylmethanesulfonate. Preinfection of Vero cells with HCMV enhanced reactivation of UV-irradiated herpes simplex virus more significantly than did treatment with UV or methylmethanesulfonate alone. A similar enhancement by HCMV was observed in human embryonic fibroblasts, but not in xeroderma pigmentosum (XP12BE) cells. It was also found that HCMV infection enhanced hydroxyurea-resistant DNA synthesis induced by UV light or methylmethanesulfonate. Alkaline sucrose gradient sedimentation analysis revealed an enhanced rate of synthesis of all size classes of DNA in UV-irradiated HCMV-infected Vero cells. However, HCMV infection did not induce repairable lesions in cellular DNA and did not significantly inhibit host cell DNA synthesis, unlike UV or methylmethanesulfonate. These results indicate that HCMV enhanced DNA repair capacity in the host cells without producing detectable lesions in cellular DNA and without inhibiting DNA synthesis. This repair appeared to be error proof for UV-damaged herpes simplex virus DNA when tested with herpes simplex virus thymidine kinase-negative mutants

  4. Emphysema Distribution and Diffusion Capacity Predict Emphysema Progression in Human Immunodeficiency Virus Infection

    Science.gov (United States)

    Leung, Janice M; Malagoli, Andrea; Santoro, Antonella; Besutti, Giulia; Ligabue, Guido; Scaglioni, Riccardo; Dai, Darlene; Hague, Cameron; Leipsic, Jonathon; Sin, Don D.; Man, SF Paul; Guaraldi, Giovanni

    2016-01-01

    Background Chronic obstructive pulmonary disease (COPD) and emphysema are common amongst patients with human immunodeficiency virus (HIV). We sought to determine the clinical factors that are associated with emphysema progression in HIV. Methods 345 HIV-infected patients enrolled in an outpatient HIV metabolic clinic with ≥2 chest computed tomography scans made up the study cohort. Images were qualitatively scored for emphysema based on percentage involvement of the lung. Emphysema progression was defined as any increase in emphysema score over the study period. Univariate analyses of clinical, respiratory, and laboratory data, as well as multivariable logistic regression models, were performed to determine clinical features significantly associated with emphysema progression. Results 17.4% of the cohort were emphysema progressors. Emphysema progression was most strongly associated with having a low baseline diffusion capacity of carbon monoxide (DLCO) and having combination centrilobular and paraseptal emphysema distribution. In adjusted models, the odds ratio (OR) for emphysema progression for every 10% increase in DLCO percent predicted was 0.58 (95% confidence interval [CI] 0.41–0.81). The equivalent OR (95% CI) for centrilobular and paraseptal emphysema distribution was 10.60 (2.93–48.98). Together, these variables had an area under the curve (AUC) statistic of 0.85 for predicting emphysema progression. This was an improvement over the performance of spirometry (forced expiratory volume in 1 second to forced vital capacity ratio), which predicted emphysema progression with an AUC of only 0.65. Conclusion Combined paraseptal and centrilobular emphysema distribution and low DLCO could identify HIV patients who may experience emphysema progression. PMID:27902753

  5. Impaired action potential initiation in GABAergic interneurons causes hyperexcitable networks in an epileptic mouse model carrying a human Na(V)1.1 mutation.

    Science.gov (United States)

    Hedrich, Ulrike B S; Liautard, Camille; Kirschenbaum, Daniel; Pofahl, Martin; Lavigne, Jennifer; Liu, Yuanyuan; Theiss, Stephan; Slotta, Johannes; Escayg, Andrew; Dihné, Marcel; Beck, Heinz; Mantegazza, Massimo; Lerche, Holger

    2014-11-05

    Mutations in SCN1A and other ion channel genes can cause different epileptic phenotypes, but the precise mechanisms underlying the development of hyperexcitable networks are largely unknown. Here, we present a multisystem analysis of an SCN1A mouse model carrying the NaV1.1-R1648H mutation, which causes febrile seizures and epilepsy in humans. We found a ubiquitous hypoexcitability of interneurons in thalamus, cortex, and hippocampus, without detectable changes in excitatory neurons. Interestingly, somatic Na(+) channels in interneurons and persistent Na(+) currents were not significantly changed. Instead, the key mechanism of interneuron dysfunction was a deficit of action potential initiation at the axon initial segment that was identified by analyzing action potential firing. This deficit increased with the duration of firing periods, suggesting that increased slow inactivation, as recorded for recombinant mutated channels, could play an important role. The deficit in interneuron firing caused reduced action potential-driven inhibition of excitatory neurons as revealed by less frequent spontaneous but not miniature IPSCs. Multiple approaches indicated increased spontaneous thalamocortical and hippocampal network activity in mutant mice, as follows: (1) more synchronous and higher-frequency firing was recorded in primary neuronal cultures plated on multielectrode arrays; (2) thalamocortical slices examined by field potential recordings revealed spontaneous activities and pathological high-frequency oscillations; and (3) multineuron Ca(2+) imaging in hippocampal slices showed increased spontaneous neuronal activity. Thus, an interneuron-specific generalized defect in action potential initiation causes multisystem disinhibition and network hyperexcitability, which can well explain the occurrence of seizures in the studied mouse model and in patients carrying this mutation. Copyright © 2014 the authors 0270-6474/14/3414874-16$15.00/0.

  6. Cupric ion reducing antioxidant capacity assay for antioxidants in human serum and for hydroxyl radical scavengers.

    Science.gov (United States)

    Apak, Reşat; Güçlü, Kubilay; Ozyürek, Mustafa; Bektaşoğlu, Burcu; Bener, Mustafa

    2010-01-01

    Tests measuring the combined antioxidant effect of the nonenzymatic defenses in biological fluids may be useful in providing an index of the organism's capability to counteract reactive species known as pro-oxidants, resist oxidative damage, and combat oxidative stress-related diseases. The selected chromogenic redox reagent for the assay of human serum should be easily accessible, stable, selective, and respond to all types of biologically important antioxidants such as ascorbic acid, alpha-tocopherol, beta-carotene, reduced glutathione (GSH), uric acid, and bilirubin, regardless of chemical type or hydrophilicity. Our recently developed cupric reducing antioxidant capacity (CUPRAC) spectrophotometric method for a number of polyphenols and flavonoids using the copper(II)-neocuproine reagent in ammonium acetate buffer is now applied to a complete series of plasma antioxidants for the assay of total antioxidant capacity of serum, and the resulting absorbance at 450 nm is recorded either directly (e.g., for ascorbic acid, alpha-tocopherol, and glutathione) or after incubation at 50 degrees C for 20 min (e.g., for uric acid, bilirubin, and albumin), quantitation being made by means of a calibration curve. The lipophilic antioxidants, alpha-tocopherol and beta-carotene, are assayed in dichloromethane. Lipophilic antioxidants of serum are extracted with n-hexane from an ethanolic solution of serum subjected to centrifugation. Hydrophilic antioxidants of serum are assayed in the centrifugate after perchloric acid precipitation of proteins. The CUPRAC molar absorptivities, linear ranges, and TEAC (trolox equivalent antioxidant capacity) coefficients of the serum antioxidants are established, and the results are evaluated in comparison with the findings of the ABTS/TEAC reference method. The intra- and inter-assay coefficients of variation (CVs) are 0.7 and 1.5%, respectively, for serum. The CUPRAC assay proved to be efficient for glutathione and thiol-type antioxidants

  7. Oxidative Damage to RPA Limits the Nucleotide Excision Repair Capacity of Human Cells.

    Science.gov (United States)

    Guven, Melisa; Brem, Reto; Macpherson, Peter; Peacock, Matthew; Karran, Peter

    2015-11-01

    Nucleotide excision repair (NER) protects against sunlight-induced skin cancer. Defective NER is associated with photosensitivity and a high skin cancer incidence. Some clinical treatments that cause photosensitivity can also increase skin cancer risk. Among these, the immunosuppressant azathioprine and the fluoroquinolone antibiotics ciprofloxacin and ofloxacin interact with UVA radiation to generate reactive oxygen species that diminish NER capacity by causing protein damage. The replication protein A (RPA) DNA-binding protein has a pivotal role in DNA metabolism and is an essential component of NER. The relationship between protein oxidation and NER inhibition was investigated in cultured human cells expressing different levels of RPA. We show here that RPA is limiting for NER and that oxidative damage to RPA compromises NER capability. Our findings reveal that cellular RPA is surprisingly vulnerable to oxidation, and we identify oxidized forms of RPA that are associated with impaired NER. The vulnerability of NER to inhibition by oxidation provides a connection between cutaneous photosensitivity, protein damage, and increased skin cancer risk. Our findings emphasize that damage to DNA repair proteins, as well as to DNA itself, is likely to be an important contributor to skin cancer risk.

  8. Education and Training Networks as a Tool for Nuclear Security Human Resource Development and Capacity Building

    International Nuclear Information System (INIS)

    Nikonov, D.

    2014-01-01

    Human Resource Development for Capacity Building for Nuclear Security: • Comprehensive Training Programme Objective: To raise awareness, to fill gaps between the actual performance of personnel and the required competencies and skills and, to build-up qualified instructors/trainers. • Promoting Nuclear Security Education Objective: To support the development of teaching material, faculty expertise and preparedness, and the promotion of nuclear security education in collaboration with the academic and scientific community. Ultimate Goal: To develop capabilities for supporting sustainable implementation of the international legal instruments and IAEA guidelines for nuclear security worldwide, and to foster nuclear security culture. Education priorities for the future: • Incorporate feedback from the first pilot program into future academic activities in nuclear security; • Based on feedback from pilot program: • Revise the NSS12 guidance document; • Update educational materials and textbooks. • Support INSEN members, which consider launching MSc programs at their institutions; • Continue promoting nuclear security education as part of existing degree programs (through certificate or concentration options); • Support the use of new forms of teaching and learning in nuclear security education: • Online e-learning degree programmes and modules; • Learning by experience; • Problem-oriented learning tailored to nuclear security functions

  9. Phenotype of transgenic mice carrying a very low copy number of the mutant human G93A superoxide dismutase-1 gene associated with amyotrophic lateral sclerosis.

    Directory of Open Access Journals (Sweden)

    Jeffrey S Deitch

    Full Text Available Amyotrophic lateral sclerosis (ALS is a progressive neurodegenerative disease of the motor neuron. While most cases of ALS are sporadic, 10% are familial (FALS with 20% of FALS caused by a mutation in the gene that codes for the enzyme Cu/Zn superoxide dismutase (SOD1. There is variability in sporadic ALS as well as FALS where even within the same family some siblings with the same mutation do not manifest disease. A transgenic (Tg mouse model of FALS containing 25 copies of the mutant human SOD1 gene demonstrates motor neuron pathology and progressive weakness similar to ALS patients, leading to death at approximately 130 days. The onset of symptoms and survival of these transgenic mice are directly related to the number of copies of the mutant gene. We report the phenotype of a very low expressing (VLE G93A SOD1 Tg carrying only 4 copies of the mutant G93ASOD1 gene. While weakness can start at 9 months, only 74% of mice 18 months or older demonstrate disease. The VLE mice show decreased motor neurons compared to wild-type mice as well as increased cytoplasmic translocation of TDP-43. In contrast to the standard G93A SOD1 Tg mouse which always develops motor weakness leading to death, not all VLE animals manifested clinical disease or shortened life span. In fact, approximately 20% of mice older than 24 months had no motor symptoms and only 18% of VLE mice older than 22 months reached end stage. Given the variable penetrance of clinical phenotype, prolonged survival, and protracted loss of motor neurons the VLE mouse provides a new tool that closely mimics human ALS. This tool will allow the study of pathologic events over time as well as the study of genetic and environmental modifiers that may not be causative, but can exacerbate or accelerate motor neuron disease.

  10. Whole body hyperthermia, but not skin hyperthermia, accelerates brain and locomotor limb circulatory strain and impairs exercise capacity in humans

    DEFF Research Database (Denmark)

    Trangmar, Steven J; Chiesa, Scott T; Kalsi, Kameljit K

    2017-01-01

    Cardiovascular strain and hyperthermia are thought to be important factors limiting exercise capacity in heat-stressed humans, however, the contribution of elevations in skin (Tsk) versus whole body temperatures on exercise capacity has not been characterized. To ascertain their relationships...... was associated with a plateau in MCA and two-legged vascular conductance (VC). Mechanistically, the falling MCA VC was coupled to reductions in PaCO2, whereas the plateau in leg vascular conductance was related to markedly elevated plasma [NA] and a plateau in plasma ATP These findings reveal that whole-body...... hyperthermia, but not skin hyperthermia, compromises exercise capacity in heat-stressed humans through the early attenuation of brain and active muscle blood flow....

  11. Characterization of in vivo chemoresistant human hepatocellular carcinoma cells with transendothelial differentiation capacities

    International Nuclear Information System (INIS)

    Marfels, Christian; Hoehn, Miriam; Wagner, Ernst; Günther, Michael

    2013-01-01

    Chemotherapeutic treatment of hepatocellular carcinoma often leads to chemoresistance during therapy or upon relapse of tumors. For the development of better treatments a better understanding of biochemical changes in the resistant tumors is needed. In this study, we focus on the characterization of in vivo chemoresistant human hepatocellular carcinoma HUH-REISO established from a metronomically cyclophosphamide (CPA) treated HUH7 xenograft model. SCID mice bearing subcutaneous HUH7 tumors were treated i.p. with 75 mg/kg CPA every six days. Tumors were evaluated by immunohistochemistry, a functional blood-flow Hoechst dye assay, and qRT-PCR for ALDH-1, Notch-1, Notch-3, HES-1, Thy-1, Oct-4, Sox-2 and Nanog mRNA levels. Cell lines of these tumors were analyzed by qRT-PCR and in endothelial transdifferentiation studies on matrigel. HUH-REISO cells, although slightly more sensitive against activated CPA in vitro than parental HUH-7 cells, fully retained their in vivo CPA chemoresistance upon xenografting into SCID mice. Histochemical analysis of HUH-REISO tumors in comparison to parental HUH-7 cells and passaged HUH-PAS cells (in vivo passaged without chemotherapeutic pressure) revealed significant changes in host vascularization of tumors and especially in expression of the tumor-derived human endothelial marker gene PECAM-1/CD31 in HUH-REISO. In transdifferentiation studies with limited oxygen and metabolite diffusion, followed by a matrigel assay, only the chemoresistant HUH-REISO cells exhibited tube formation potential and expression of human endothelial markers ICAM-2 and PECAM-1/CD31. A comparative study on stemness and plasticity markers revealed upregulation of Thy-1, Oct-4, Sox-2 and Nanog in resistant xenografts. Under therapeutic pressure by CPA, tumors of HUH-PAS and HUH-REISO displayed regulations in Notch-1 and Notch-3 expression. Chemoresistance of HUH-REISO was not manifested under standard in vitro but under in vivo conditions. HUH-REISO cells showed

  12. Overview of Recent Activities on Safety Culture and Human and Organizational Factors Carried Out at the Joint Research Centre of the European Commission

    International Nuclear Information System (INIS)

    Stručić, M.; Manna, G.

    2016-01-01

    The Institute for Energy and Transport (IET) of the Joint Research Centre (JRC) of the European Commission (EC) is since more than ten years active in the field of Safety Culture (SC) and Human and Organizational Factors (HOF). Several activities related to SC and HOF have been and are carried out in the frame of the EU Nuclear Safety Clearinghouse for Operating Experience Feedback (Clearinghouse). The Clearinghouse was established in 2008 to enhance nuclear safety through the lessons learned from NPP events, and to provide help in Operational Experience Feedback (OEF) process primarily to nuclear safety Regulatory Authorities and to their Technical Support Organizations within the EU. Additionally to these activities, during the Fukushima accident, Clearinghouse has been regularly providing reports on the status and progress of the accident to the EU Regulatory Authorities. Moreover, experts, selected from the JRC staffing, were directly engaged in the EU-wide risk and safety assessments of nuclear power plants known as “the Stress Tests”.

  13. Murine polyomavirus virus-like particles carrying full-length human PSA protect BALB/c mice from outgrowth of a PSA expressing tumor.

    Directory of Open Access Journals (Sweden)

    Mathilda Eriksson

    Full Text Available Virus-like particles (VLPs consist of capsid proteins from viruses and have been shown to be usable as carriers of protein and peptide antigens for immune therapy. In this study, we have produced and assayed murine polyomavirus (MPyV VLPs carrying the entire human Prostate Specific Antigen (PSA (PSA-MPyVLPs for their potential use for immune therapy in a mouse model system. BALB/c mice immunized with PSA-MPyVLPs were only marginally protected against outgrowth of a PSA-expressing tumor. To improve protection, PSA-MPyVLPs were co-injected with adjuvant CpG, either alone or loaded onto murine dendritic cells (DCs. Immunization with PSA-MPyVLPs loaded onto DCs in the presence of CpG was shown to efficiently protect mice from tumor outgrowth. In addition, cellular and humoral immune responses after immunization were examined. PSA-specific CD4(+ and CD8(+ cells were demonstrated, but no PSA-specific IgG antibodies. Vaccination with DCs loaded with PSA-MPyVLPs induced an eight-fold lower titre of anti-VLP antibodies than vaccination with PSA-MPyVLPs alone. In conclusion, immunization of BALB/c mice with PSA-MPyVLPs, loaded onto DCs and co-injected with CpG, induces an efficient PSA-specific tumor protective immune response, including both CD4(+ and CD8(+ cells with a low induction of anti-VLP antibodies.

  14. Establishment of a new human pre-B acute lymphoblastic leukemia cell line (KMO-90) with 1;19 translocation carrying p53 gene alterations.

    Science.gov (United States)

    Sotomatsu, M; Hayashi, Y; Kawamura, M; Yugami, S; Shitara, T

    1993-10-01

    A new human pre-B acute lymphoblastic leukemia cell line (KMO-90) was established from the bone marrow sample of a 12-year-old girl with acute lymphoblastic leukemia (ALL) carrying 1;19 chromosome translocation. KMO-90 cells expressed HLA-DR, CD10, CD19, and CD22 antigens. These cells had also cytoplasmic immunoglobulin lacking surface immunoglobulin, indicating that these had a pre-B phenotype. Chromosome analysis of this cell line showed 48, XX, +8, +19, t(1;19)(q23;p13). Southern blot analysis showed the same sized rearrangements of the E2A gene in KMO-90 cells as those in the original leukemic cells. By means of reverse transcriptase-polymerase chain reaction analysis, we detected E2A/PBX1 fusion transcripts in KMO-90 cells. KMO-90 is useful when studying the role of the 1;19 translocation in the etiology of pre-B ALL. Furthermore, we studied alterations of the p53 gene in this cell line by polymerase chain reaction, single-strand conformation polymorphism analysis. KMO-90 cells were identified to have a point mutation at codon 177 (CCC-->TCC) of the p53 gene, suggesting that alterations of the p53 gene may have an important role in the establishment of this cell line.

  15. Murine Polyomavirus Virus-Like Particles Carrying Full-Length Human PSA Protect BALB/c Mice from Outgrowth of a PSA Expressing Tumor

    Science.gov (United States)

    Eriksson, Mathilda; Andreasson, Kalle; Weidmann, Joachim; Lundberg, Kajsa; Tegerstedt, Karin

    2011-01-01

    Virus-like particles (VLPs) consist of capsid proteins from viruses and have been shown to be usable as carriers of protein and peptide antigens for immune therapy. In this study, we have produced and assayed murine polyomavirus (MPyV) VLPs carrying the entire human Prostate Specific Antigen (PSA) (PSA-MPyVLPs) for their potential use for immune therapy in a mouse model system. BALB/c mice immunized with PSA-MPyVLPs were only marginally protected against outgrowth of a PSA-expressing tumor. To improve protection, PSA-MPyVLPs were co-injected with adjuvant CpG, either alone or loaded onto murine dendritic cells (DCs). Immunization with PSA-MPyVLPs loaded onto DCs in the presence of CpG was shown to efficiently protect mice from tumor outgrowth. In addition, cellular and humoral immune responses after immunization were examined. PSA-specific CD4+ and CD8+ cells were demonstrated, but no PSA-specific IgG antibodies. Vaccination with DCs loaded with PSA-MPyVLPs induced an eight-fold lower titre of anti-VLP antibodies than vaccination with PSA-MPyVLPs alone. In conclusion, immunization of BALB/c mice with PSA-MPyVLPs, loaded onto DCs and co-injected with CpG, induces an efficient PSA-specific tumor protective immune response, including both CD4+ and CD8+ cells with a low induction of anti-VLP antibodies. PMID:21858228

  16. Human decellularized bone scaffolds from aged donors show improved osteoinductive capacity compared to young donor bone.

    Directory of Open Access Journals (Sweden)

    Christopher A Smith

    Full Text Available To improve the safe use of allograft bone, decellularization techniques may be utilized to produce acellular scaffolds. Such scaffolds should retain their innate biological and biomechanical capacity and support mesenchymal stem cell (MSC osteogenic differentiation. However, as allograft bone is derived from a wide age-range, this study aimed to determine whether donor age impacts on the ability an osteoinductive, acellular scaffold produced from human bone to promote the osteogenic differentiation of bone marrow MSCs (BM-MSC. BM-MSCs from young and old donors were seeded on acellular bone cubes from young and old donors undergoing osteoarthritis related hip surgery. All combinations resulted in increased osteogenic gene expression, and alkaline phosphatase (ALP enzyme activity, however BM-MSCs cultured on old donor bone displayed the largest increases. BM-MSCs cultured in old donor bone conditioned media also displayed higher osteogenic gene expression and ALP activity than those exposed to young donor bone conditioned media. ELISA and Luminex analysis of conditioned media demonstrated similar levels of bioactive factors between age groups; however, IGF binding protein 1 (IGFBP1 concentration was significantly higher in young donor samples. Additionally, structural analysis of old donor bone indicated an increased porosity compared to young donor bone. These results demonstrate the ability of a decellularized scaffold produced from young and old donors to support osteogenic differentiation of cells from young and old donors. Significantly, the older donor bone produced greater osteogenic differentiation which may be related to reduced IGFBP1 bioavailability and increased porosity, potentially explaining the excellent clinical results seen with the use of allograft from aged donors.

  17. 基于改进AHP的旅游环境承载力评价研究--以鼓浪屿景区为例%Assessment of Tourist Environment Carrying Capacity Based on Improved AHP:a Case Study of Gulangyu Island Scenic Spot

    Institute of Scientific and Technical Information of China (English)

    贾志涛; 曾繁英

    2017-01-01

    Tourist environment carrying capacity must be confirmed for protecting the ecological environment, realizing a sustainable de-velopment of sightseeing districts, managing tourist activities in scenic spots effectively, and regulating the development and utilization of tourism resources. The environment carrying capacity data of Gulangyu island on weekdays and holidays is analyzed with improved AHP by establishing an index system for assessing the environmental carrying capacity, combining the natural ecology, social psychology and e-conomic environment carrying capacity. The analysis finds out that the scenic spot should enhance the management of long-term over ca-pacity of tourists, the improvement of the atmosphere purifying capacity, pollutant disposal processing capacity, and the restrictive factors of the psychological bearing capacity of tourists. By doing so, the tourist environment carrying capacity could be promoted with tourist number controlled at an acceptable level, facilitating the sustainable development of Gulangyu island.%为了实现旅游地的生态环境保护及可持续发展目标,有效管理景区游客活动和调控旅游资源的开发利用,必须确定景区的旅游资源环境承载力。通过构建兼顾自然生态环境承载力、社会心理承载力和经济环境承载力三个维度的环境承载力评价指标体系,运用改进的层次分析模型,对景区旅游环境承载力进行量化测算和评价,分析鼓浪屿景区在工作日和节假日的旅游环境承载力数据结果,鼓浪屿景区的可持续发展既需要根据景区旅游环境承载力对当前的长期游客过载现象加强治理,也需要重视大气净化能力、污染物处理能力、游客心理承载力限制性要素的改善以提高景区自身的旅游环境承载力,把景区的旅游数量控制在可接受范围内,推动鼓浪屿景区的持续发展。

  18. Adult and Non-Formal Education: An Imperative for Human Capacity ...

    African Journals Online (AJOL)

    DR Nneka

    capacity development could be defined as the development of a workforce .... and the community and between theory and practice in Adult Education, ... ANFE provides education for women in order to empower them, enable them gain.

  19. From smallpox eradication to contemporary global health initiatives: enhancing human capacity towards a global public health goal.

    Science.gov (United States)

    Tarantola, Daniel; Foster, Stanley O

    2011-12-30

    The eradication of smallpox owes its success first and foremost to the thousands of lay health workers and community members who, throughout the campaign and across continents, took on the roles of advocates, educators, vaccinators, care providers and contributors to epidemic surveillance and containment. Bangladesh provides a good example where smallpox eradication and the capacity enhancement needed to achieve this goal resulted in a two-way mutually beneficial process. Smallpox-dedicated staff provided community members with information guidance, support and tools. In turn, communities not only created the enabling environment for smallpox program staff to perform their work but acquired the capacity to perform essential eradication tasks. Contemporary global health programmes can learn much from these core lessons including: the pivotal importance of supporting community aspirations, capacity and resilience; the critical need to enhance commitment, capacity and accountability across the workforce; and the high value of attentive human resources management and support. We owe to subsequent global disease control, elimination and eradication ventures recognition of the need for social and behavioural science to inform public health strategies; the essential roles that civil society organizations and public-private partnerships can play in public health discourse and action; the overall necessity of investing in broad-based health system strengthening; and the utility of applying human rights principles, norms and standards to public health policy and practice. Crown Copyright © 2011. Published by Elsevier Ltd. All rights reserved.

  20. Assessments of ecosystem services and human well-being in Thailand build and create demand for coproductive capacity

    Directory of Open Access Journals (Sweden)

    Louis Lebel

    2015-03-01

    Full Text Available Assessments of ecosystem services have been proposed as one way of incorporating concerns about environmental change and ecosystem conditions into subnational development planning. In Thailand a policy window for such initiatives is opening because of a transition in national policy toward area-based planning combined with broader political reforms to expand public participation and encourage more evidence-based decision making. We explored three case studies in Thailand in which central and local government agencies and research organizations partnered to engage local communities and other stakeholders in assessments of ecosystem services and human well-being. The analysis focused on the role ecosystem assessments play in building and creating demand for coproductive capacity. By coproductive capacities we mean the ability to combine scientific resources and governance capabilities in ways that bring about informed social change. We found evidence that the assessments built capacities for governance actors to explore scientific and research-based evidence, to consult scientific experts, and then to evaluate existing policies and plans using this newly acquired information. At the same time, scientific experts also learned to explore public policy issues, to consult planners and decision makers in government, and based on this knowledge to evaluate scientific evidence and revise the scope and goals of their research and analytical activities to better meet policy needs and demands. Coproductive capacities were built when various stakeholders jointly engaged in compilation and interpretation of evidence. Doing so helped legitimize the assessment process with positive feedback on both governance and science capacities. We also found evidence, however, of significant cultural and institutional constraints to designing and making better use of ecosystem services assessments. These constraints included insufficient resources for both knowledge making

  1. SLC2A9 is a high-capacity urate transporter in humans.

    Directory of Open Access Journals (Sweden)

    Mark J Caulfield

    2008-10-01

    Full Text Available Serum uric acid levels in humans are influenced by diet, cellular breakdown, and renal elimination, and correlate with blood pressure, metabolic syndrome, diabetes, gout, and cardiovascular disease. Recent genome-wide association scans have found common genetic variants of SLC2A9 to be associated with increased serum urate level and gout. The SLC2A9 gene encodes a facilitative glucose transporter, and it has two splice variants that are highly expressed in the proximal nephron, a key site for urate handling in the kidney. We investigated whether SLC2A9 is a functional urate transporter that contributes to the longstanding association between urate and blood pressure in man.We expressed both SLC2A9 splice variants in Xenopus laevis oocytes and found both isoforms mediate rapid urate fluxes at concentration ranges similar to physiological serum levels (200-500 microM. Because SLC2A9 is a known facilitative glucose transporter, we also tested whether glucose or fructose influenced urate transport. We found that urate is transported by SLC2A9 at rates 45- to 60-fold faster than glucose, and demonstrated that SLC2A9-mediated urate transport is facilitated by glucose and, to a lesser extent, fructose. In addition, transport is inhibited by the uricosuric benzbromarone in a dose-dependent manner (Ki = 27 microM. Furthermore, we found urate uptake was at least 2-fold greater in human embryonic kidney (HEK cells overexpressing SLC2A9 splice variants than nontransfected kidney cells. To confirm that our findings were due to SLC2A9, and not another urate transporter, we showed that urate transport was diminished by SLC2A9-targeted siRNA in a second mammalian cell line. In a cohort of men we showed that genetic variants of SLC2A9 are associated with reduced urinary urate clearance, which fits with common variation at SLC2A9 leading to increased serum urate. We found no evidence of association with hypertension (odds ratio 0.98, 95% confidence interval [CI

  2. 基于5A景区最大承载量和游客接待量的旅游供需关系模型研究%Study of Mathematical Model Between Tourism Supply and Demand Based on Relationship of the 5A Level Scenic Spots' Maximum Carrying Capacity and the Amount of the Tourists

    Institute of Scientific and Technical Information of China (English)

    刘静; 刘耀龙; 段锦

    2017-01-01

    旅游承载量是反映旅游供给能力的重要指标,游客接待量是衡量旅游需求状况的常用指标.在分析我国5A级景区最大承载量空间分异特征的基础上,对2014年各省市5A级景区日最大承载量和年游客接待量进行相关性和回归分析,构建基于景区承载量和游客接待量的旅游供需关系模型.结果表明:(1)5A级景区最大承载量和游客接待量之间存在显著的正相关关系;(2)旅游供需关系模型可由复合函数定量表征;(3)模型能够预测旅游供需变化,对旅游业发展具有一定的指示意义.%Tourism carrying capacity is an important index to reflect the tourism supply capacity, and the amount of the tourists is a common index to measure tourism demand. On the basis of the analysis of the spatial distribution characteristics of 5A level scenic spots' maximum carrying capacity, the paper carries out correlation and regression analysis about the 5A level scenic' maximum carrying capacity and the amount of the tourists. Sample data is calculated or statistical results of 31 provinces in 2014. Tourism supply and demand model is constructed based on the relationship between the 5A level scenic spots' maximum carrying capacity and the amount of the tourists. Results indicate: (1)There is a significant positive correlation between the 5A level scenic spot'maximum carrying capacity and the amount of tourists; (2)The model of tourism supply and demand relations can be quantitatively characterized by complex functions;(3)The model can predict the change of tourism supply and demand, which have referential meaning to the development of tourism.

  3. Legal capacity as a universal human right and a determinant of social status of people with mental disability

    Directory of Open Access Journals (Sweden)

    Marković Milan M.

    2012-01-01

    Full Text Available Adoption of the UN Convention on the Right of Persons with Disabilities (2006 brought about a core shift to how the international community and human rights law see and treat human disability in general. This paradigm shift materilizes itself in a number of provisions ranging from those which catalogue the proclaimed human rights as they are in the context of special implementation and protection of people with disabilities, to those that introduce a level of specificity in light of their holders' particular needs. But the strongest presence of the shift to this regard can be found in the Article 12 CRPD that sheds new light on the concept of (legal capacity of people with (mental disabilites. According to this norm and put quite simply - there should be no difference in observing and treating capacity of a person with disabilities to that of any other person. This is not only the matter of prohibiting discrimination on grounds of mental impairments, but furthermore preventing the system from establishing a classification in which a person with psychosocial or intellectual impairment would be a second-rate citizen, an object of law or a victim of legal, social and family abuse, someone who is a burden to his entire environment, someone who does not have a say in any case concerning his own life and wellbeing. Legal capacity should not be a goal to be fighting for, but a universal human right. Of course and unfortunatelly, such a shift is purely a formal one, when not causing due reform within the national systems and without proper implementation in the member states. What is thought urgently needed and directly required by the given provision is removing the system features that allow deprivation of legal capacity on the bases of mental impairments and introducing a humane and human rights oriented model in which the decision making of these people would be autonomous and supported, and with only very restricted exceptions, done by them and not

  4. Institutional capacity for health systems research in East and Central African Schools of Public Health: strengthening human and financial resources

    Science.gov (United States)

    2014-01-01

    Background Despite its importance in providing evidence for health-related policy and decision-making, an insufficient amount of health systems research (HSR) is conducted in low-income countries (LICs). Schools of public health (SPHs) are key stakeholders in HSR. This paper, one in a series of four, examines human and financial resources capacities, policies and organizational support for HSR in seven Africa Hub SPHs in East and Central Africa. Methods Capacity assessment done included document analysis to establish staff numbers, qualifications and publications; self-assessment using a tool developed to capture individual perceptions on the capacity for HSR and institutional dialogues. Key informant interviews (KIIs) were held with Deans from each SPH and Ministry of Health and non-governmental officials, focusing on perceptions on capacity of SPHs to engage in HSR, access to funding, and organizational support for HSR. Results A total of 123 people participated in the self-assessment and 73 KIIs were conducted. Except for the National University of Rwanda and the University of Nairobi SPH, most respondents expressed confidence in the adequacy of staffing levels and HSR-related skills at their SPH. However, most of the researchers operate at individual level with low outputs. The average number of HSR-related publications was only capacity. This study underscores the need to form effective multidisciplinary teams to enhance research of immediate and local relevance. Capacity strengthening in the SPH needs to focus on knowledge translation and communication of findings to relevant audiences. Advocacy is needed to influence respective governments to allocate adequate funding for HSR to avoid donor dependency that distorts local research agenda. PMID:24888371

  5. Public-private partnerships to build human capacity in low income countries: findings from the Pfizer program

    Directory of Open Access Journals (Sweden)

    Connelly Patrick

    2007-03-01

    Full Text Available Abstract Background The ability of health organizations in developing countries to expand access to quality services depends in large part on organizational and human capacity. Capacity building includes professional development of staff, as well as efforts to create working environments conducive to high levels of performance. The current study evaluated an approach to public-private partnership where corporate volunteers give technical assistance to improve organizational and staff performance. From 2003 to 2005, the Pfizer Global Health Fellows program sent 72 employees to work with organizations in 19 countries. This evaluation was designed to assess program impact. Methods The researchers administered a survey to 60 Fellows and 48 Pfizer Supervisors. In addition, the team conducted over 100 interviews with partner organization staff and other key informants during site visits in Uganda, Kenya, Ghana, South Africa and India, the five countries where 60% of Fellows were placed. Results Over three-quarters of Fellowships appear to have imparted skills or enhanced operations of NGOs in HIV/AIDS and other health programs. Overall, 79% of Fellows reported meeting all or most technical assistance goals. Partner organization staff reported that the Fellows provided training to clinical and research personnel; strengthened laboratory, pharmacy, financial control, and human resource management systems; and helped expand Partner organization networks. Local staff also reported the Program changed their work habits and attitudes. The evaluation identified problems in defining goals of Fellowships and matching Organizations with Fellows. Capacity building success also appears related to size and sophistication of partner organization. Conclusion Public expectations have grown regarding the role corporations should play in improving health systems in developing countries. Corporate philanthropy programs based on "donations" of personnel can help build

  6. Public-private partnerships to build human capacity in low income countries: findings from the Pfizer program.

    Science.gov (United States)

    Vian, Taryn; Richards, Sarah C; McCoy, Kelly; Connelly, Patrick; Feeley, Frank

    2007-03-02

    The ability of health organizations in developing countries to expand access to quality services depends in large part on organizational and human capacity. Capacity building includes professional development of staff, as well as efforts to create working environments conducive to high levels of performance. The current study evaluated an approach to public-private partnership where corporate volunteers give technical assistance to improve organizational and staff performance. From 2003 to 2005, the Pfizer Global Health Fellows program sent 72 employees to work with organizations in 19 countries. This evaluation was designed to assess program impact. The researchers administered a survey to 60 Fellows and 48 Pfizer Supervisors. In addition, the team conducted over 100 interviews with partner organization staff and other key informants during site visits in Uganda, Kenya, Ghana, South Africa and India, the five countries where 60% of Fellows were placed. Over three-quarters of Fellowships appear to have imparted skills or enhanced operations of NGOs in HIV/AIDS and other health programs. Overall, 79% of Fellows reported meeting all or most technical assistance goals. Partner organization staff reported that the Fellows provided training to clinical and research personnel; strengthened laboratory, pharmacy, financial control, and human resource management systems; and helped expand Partner organization networks. Local staff also reported the Program changed their work habits and attitudes. The evaluation identified problems in defining goals of Fellowships and matching Organizations with Fellows. Capacity building success also appears related to size and sophistication of partner organization. Public expectations have grown regarding the role corporations should play in improving health systems in developing countries. Corporate philanthropy programs based on "donations" of personnel can help build the organizational and human capacity of frontline agencies

  7. Contribution of International and Regional Networks in Developing and Maintaining Human Capacity Building for Nuclear Power Programmes

    International Nuclear Information System (INIS)

    Osman, O. E.

    2015-01-01

    Capacity is defined as; the ability of individuals and organizations or organizational units to perform functions effectively, efficiently and sustainably. Capacity building is an evidence-driven process of strengthening the abilities of individuals, organizations, and systems to perform core functions sustainably, and to continue to improve and develop over time. This article will explain the contributions of knowledge networks at the national, regional and international level in developing the existing capacity building and human resources for regulatory body in Sudan, to confront the future challenges regarding to nuclear power program- safety and security. The article will compare the advantages and effectiveness of these knowledge networks (IAEA, ANNuR, FNRBA) in capacity building and enhance the infrastructure of national regulatory body. And how these networks contribute to enable the regulatory bodies in Africa and Arab countries, to establish and strengthen their regulatory infrastructure for nuclear power programme consistent with international standards and recommendations. As well as the recommendations resulting and deduced from comparative study to promote the exchange of knowledge, experience and information among its members. (author)

  8. Mapping of Vegetation with the Geoinformation System and Determining of Carrying Capacity of the Pre-Urals Steppe area for a Newly Establishing Population of the Przewalski Horse Equus ferus przewalskii at the Orenburg State Nature Reserve

    Science.gov (United States)

    Fedorov, N. I.; Mikhailenko, O. I.; Zharkikh, T. L.; Bakirova, R. T.

    2018-01-01

    Mapping of the vegetation (1:25000) of the Pre-Urals Steppe area at the Orenburg State Nature Reserve was completed in 2016. A map created with the geoinformation system contains 1931 simple and complex polygons for 25 types of vegetation. In a drought year, the average stock of palatable vegetation of the whole area is estimated at 8380 tons dry weight. The estimation is based on the size of areas covered by different types of vegetation, their grass production, the correction coefficients for decreasing of pasture forage stocks in winter and decreasing of production of grass communities in dry years. Based on pasture forage stocks the area could tolerate the maximum population size of 1769 individuals of the Przewalski horse, their average density could be 0.11 horse per ha. Yet, as watering places for animals are limited in Pre-Urals Steppe, grazing pressures on the vegetation next to the water sources may increase in dry years. That is why the above-mentioned calculated maximum population size and density must be reduced at least by half until some additional watering places are established and monitoring of the grazing effect on the vegetation next to the places is carried out regularly. Thus, the maximum size of the population is estimated at 800 to 900 individuals, which is almost 1.5 times more than necessary to establish a self-sustained population of the Przewalski horse.

  9. Research of the Tourism Destination Carrying Capacity---A Case Study of the Ancient City of Kaifeng Song Du Cultural Industry Park%旅游目的地容量测量研究--以开封宋都古城文化产业园区为例

    Institute of Scientific and Technical Information of China (English)

    孙慧娟

    2016-01-01

    随着旅游市场的快速发展和游客出游的不断增加,过多游客的涌入、景区超载拥堵现象不断出现。旅游容量作为旅游目的地规划开发的科学依据和景区管理的工具,其研究成为一个重要课题。依据木桶原理及限制性因子原理,选取空间容量、设施容量、生态容量和社会心理容量等作为旅游地容量指标,构建综合容量测量模型。以开封宋都古城文化产业园区为例,选取基本空间标准,分析得出产业园区日旅游容量为190484人次,年旅游容量69526660人次,并对产业园区旅游发展提出建议。%With the rapid development of the tourism market and the increasing of tourists ,the influx of too many tourists 、the scenic spots overload and congestions phenomenons appear constantly .Tourism carrying capacity is a scientific basis of tourism destination planning development and a tool of the scenic area management ,soit has become an important subject .Based on buckets effect and the principle of limiting factors , this paper selects space capacity , facilities capacity,ecological capacity and social psychological capacity as the basic indexes of tourism carrying capacity , and constructs comprehensive capacity measurement model.Then,this paper takes the ancient city of Kaifeng Song Du cultural industry park as an example,and selects basic space standards,analyzes and concludes that the tourism carrying capacity of industry park is 190484 person/time every-day,69526660 person/time every year,finally puts forward the proposal on its tourism development .

  10. Optical Carry Adder.

    Science.gov (United States)

    1987-03-01

    AOM’s) with the deflected beam as the modulator "on" state. These AOM’s ( TeO2 crystals, manufactured by Newport E.O. Systems) have high deflection...caused by the slow acoustic propagation (4.2 - 105 cm/s for TeO2 ), but this delay can be minimized by placing the laser beam close to the acoustic...dependent jitter in the optical carry to below 1 ns, the total carry path must be less than 30 cm long (or 20 cm in glass , 14 cm in LiNbO 3). Thus, a 32

  11. Research on Associated Action between Tourism Industrial Agglomeration Development and Carrying Capacity Ascension in China′s Coastal Regions%我国沿海旅游产业集聚发展与承载力提升关联作用研究

    Institute of Scientific and Technical Information of China (English)

    刘佳; 王娟

    2016-01-01

    在剖析旅游产业集聚与旅游环境承载力关联作用的基础上,对沿海地区旅游产业集聚程度及旅游环境承载力综合指数进行测度,揭示二者的动态变化与空间差异特征;进而对2001-2012年承载能力与集聚程度指数的时间序列数据进行计量检验,构建两个变量的向量自回归模型( VAR),分析沿海地区旅游产业集聚程度与旅游环境承载力水平之间的动态效应关系。研究表明:旅游产业集聚与旅游环境承载力存在长期稳定的相关性;从二者动态关系变化上看,在特定空间和时间范围内的旅游环境承载力对旅游产业集聚发展产生了限制作用和负向影响;随着规模效应、成本效应与竞争优势的发挥,旅游产业集聚会推动区域旅游环境承载水平的提升,承载力对集聚的影响转化为正向影响;同时旅游产业集聚对旅游环境承载力变化的贡献水平远大于旅游环境承载力对集聚变化的贡献水平。%On the basis of analyzing related mechanism of tourism industrial agglomeration and carrying capacity, the pa-per measures tourism industrial concentration degree and tourism environment carrying capacity index in coastal regions to reveal the characteristics of dynamic change and space difference between them;then it tests the time series data of carry-ing capacity and concentration degree index from 2001 to 2012 and builds vector auto-regressive model ( VAR) to ana-lyze the dynamic effect between tourism industrial concentration degree and tourism environment carrying capacity index in coastal regions.The results show that:there is long-term stable relationship between them;carrying capacity has neg-ative impact on tourism industry agglomeration, but it will be transformed into positive influence alongside regional tourism carrying capacity ascension by the scale effect, cost effect and competitive advantage;the industry agglomeration

  12. 单相电流对三芯钢带铠装电缆载流量试验结果影响程度的研究%Effect of Single-phase Current on the Current-carrying Capacity of Three-core Steel Armoured Cable

    Institute of Scientific and Technical Information of China (English)

    牛海清; 张群峰; 游勇; 赵继光; 张尧

    2014-01-01

    采用剥掉铠装层通以单相电流模拟三芯钢带铠装电缆通以三相电流,将敷设于电缆沟中的完整电缆段和剥掉铠装层电缆段串联加载不同的电流进行载流量试验,对比研究了两者的温度;根据电磁场理论,计算了载流量试验中的铠装损耗。按照IEC载流量计算标准,研究了铠装损耗对载流量影响的程度。研究表明,三芯铠装电缆加载单相电流时的铠装损耗不可忽略,但对载流量的影响不超过4%,且偏保守。%The current-carrying capacity of three-core cable with and without steel armour layed in cable trench was tested through applying different single-phase current, and their temperature was compared. The loss induced by armour of the test cable was calculated according to electromagnetic field theory. The effect of loss induced by armour on the current-carrying capacity was studied according to IEC standard of current-carrying capacity. The results show that the loss induced by armour of the three-core cable can’t be neglected when applying single-phase current, and the current-carrying capacity decreases by about 4%, which is acceptable.

  13. The Chinese version of monitoring and evaluation system strengthening tool for human immunodeficiency virus (HIV) capacity building: Development and evaluation.

    Science.gov (United States)

    Zhao, Ran; Chen, Ren; Zhang, Bing; Ma, Ying; Qin, Xia; Hu, Zhi

    2015-08-01

    Monitoring and evaluation (M&E) for human immunodeficiency virus (HIV) capacity building has become a significant step for HIV prevention and control. The M&E system strengthening tool published by the United Nations Joint Programme on HIV/AIDS (UNAIDS) was intended to be the most authoritative assessment tool internationally. Facing the fact that the M&E system in China did not function at an optimum level, we considered taking the international standards for reference. By linguistic validating and different stages' discussions and revisions, we came up with the Chinese version of the capacity diagnosis tool with at least 12 components and tested its validity and reliability. The tool turned out to have a sufficiently linguistic validation and proved to be a scientific and feasible instrument which was suitable for China's national conditions.

  14. Association between in vivo bone formation and ex vivo migratory capacity of human bone marrow stromal cells

    DEFF Research Database (Denmark)

    Andersen, Rikke K.; Zaher, Walid; Larsen, Kenneth Hauberg

    2015-01-01

    INTRODUCTION: There is a clinical need for developing systemic transplantation protocols for use of human skeletal stem cells (also known bone marrow stromal stem cells) (hBMSC) in tissue regeneration. In systemic transplantation studies, only a limited number of hBMSC home to injured tissues...... populations derived from telomerized hBMSC (hBMSC-TERT) with variable ability to form heterotopic bone when implanted subcutaneously in immune deficient mice. In vitro transwell migration assay was used and the in vivo homing ability of transplanted hBMSC to bone fractures in mice was visualized...... suggesting that only a subpopulation of hBMSC possesses "homing" capacity. Thus, we tested the hypothesis that a subpopulation of hBMSC defined by ability to form heterotopic bone in vivo, is capable of homing to injured bone. METHODS: We tested ex vivo and in vivo homing capacity of a number of clonal cell...

  15. Secretion of human epidermal growth factor (EGF) in autotrophic culture by a recombinant hydrogen-utilizing bacterium, Pseudomonas pseudoflava, carrying broad-host-range EGF secretion vector pKSEGF2.

    OpenAIRE

    Hayase, N; Ishiyama, A; Niwano, M

    1994-01-01

    We constructed the broad-host-range human epidermal growth factor (EGF) secretion plasmid pKSEGF2 by inserting the Escherichia coli tac promoter, the signal sequence of Pseudomonas stutzeri amylase, and the synthesized EGF gene into the broad-host-range vector pKT230. E. coli JM109 carrying pKSEGF2 secreted EGF into the periplasm and the culture medium under the control of the tac promoter. Pseudomonas aeruginosa PAO1161 carrying pKSEGF2 and Pseudomonas putida AC10 carrying pKSEGF2 secreted E...

  16. Cancer-associated fibroblasts from human NSCLC survive ablative doses of radiation but their invasive capacity is reduced

    Directory of Open Access Journals (Sweden)

    Hellevik Turid

    2012-04-01

    Full Text Available Abstract Background Cancer-Associated Fibroblasts (CAFs are significant components of solid malignancies and play central roles in cancer sustainability, invasion and metastasis. In this study we have investigated the invasive capacity and matrix remodelling properties of human lung CAFs after exposure to ablative doses of ionizing radiation (AIR, equivalent to single fractions delivered by stereotactic ablative radiotherapy (SART for medically inoperable stage-I/II non-small-cell lung cancers. Methods CAFs were isolated from lung tumour specimens from 16 donors. Initially, intrinsic radiosensitivity was evaluated by checking viability and extent of DNA-damage response (DDR at different radiation doses. The migrative and invasive capacities of CAFs were thereafter determined after a sub-lethal single radiation dose of 18 Gy. To ascertain the mechanisms behind the altered invasive capacity of cells, expression of matrix metalloproteinases (MMPs and their endogenous inhibitors (TIMPs were measured in the conditioned media several days post-irradiation, along with expression of cell surface integrins and dynamics of focal contacts by vinculin-staining. Results Exposing CAFs to 1 × 18 Gy resulted in a potent induction of multiple nuclear DDR foci (> 9/cell with little resolution after 120 h, induced premature cellular senescence and inhibition of the proliferative, migrative and invasive capacity. AIR promoted MMP-3 and inhibited MMP-1 appearance to some extent, but did not affect expression of other major MMPs. Furthermore, surface expression of integrins α2, β1 and α5 was consistently enhanced, and a dramatic augmentation and redistribution of focal contacts was observed. Conclusions Our data indicate that ablative doses of radiation exert advantageous inhibitory effects on the proliferative, migratory and invasive capacity of lung CAFs. The reduced motility of irradiated CAFs might be a consequence of stabilized focal contacts via integrins.

  17. Robust Lentiviral Gene Delivery But Limited Transduction Capacity of Commonly Used Adeno-Associated Viral Serotypes in Xenotransplanted Human Skin.

    Science.gov (United States)

    Jakobsen, Maria; Askou, Anne Louise; Stenderup, Karin; Rosada, Cecilia; Dagnæs-Hansen, Frederik; Jensen, Thomas G; Corydon, Thomas J; Mikkelsen, Jacob Giehm; Aagaard, Lars

    2015-08-01

    Skin is an easily accessible organ, and therapeutic gene transfer to skin remains an attractive alternative for the treatment of skin diseases. Although we have previously documented potent lentiviral gene delivery to human skin, vectors based on adeno-associated virus (AAV) rank among the most promising gene delivery tools for in vivo purposes. Thus, we compared the potential usefulness of various serotypes of recombinant AAV vectors and lentiviral vectors for gene transfer to human skin in a xenotransplanted mouse model. Vector constructs encoding firefly luciferase were packaged in AAV capsids of serotype 1, 2, 5, 6, 8, and 9 and separately administered by intradermal injection in human skin transplants. For all serotypes, live bioimaging demonstrated low levels of transgene expression in the human skin graft, and firefly luciferase expression was observed primarily in neighboring tissue outside of the graft. In contrast, gene delivery by intradermally injected lentiviral vectors was efficient and led to extensive and persistent firefly luciferase expression within the human skin graft only. The study demonstrates the limited capacity of single-stranded AAV vectors of six commonly used serotypes for gene delivery to human skin in vivo.

  18. Variant at serotonin transporter gene predicts increased imitation in toddlers: relevance to the human capacity for cumulative culture.

    Science.gov (United States)

    Schroeder, Kari Britt; Asherson, Philip; Blake, Peter R; Fenstermacher, Susan K; Saudino, Kimberly J

    2016-04-01

    Cumulative culture ostensibly arises from a set of sociocognitive processes which includes high-fidelity production imitation, prosociality and group identification. The latter processes are facilitated by unconscious imitation or social mimicry. The proximate mechanisms of individual variation in imitation may thus shed light on the evolutionary history of the human capacity for cumulative culture. In humans, a genetic component to variation in the propensity for imitation is likely. A functional length polymorphism in the serotonin transporter gene, the short allele at 5HTTLPR, is associated with heightened responsiveness to the social environment as well as anatomical and activational differences in the brain's imitation circuity. Here, we evaluate whether this polymorphism contributes to variation in production imitation and social mimicry. Toddlers with the short allele at 5HTTLPR exhibit increased social mimicry and increased fidelity of demonstrated novel object manipulations. Thus, the short allele is associated with two forms of imitation that may underlie the human capacity for cumulative culture. The short allele spread relatively recently, possibly due to selection, and its frequency varies dramatically on a global scale. Diverse observations can be unified via conceptualization of 5HTTLPR as influencing the propensity to experience others' emotions, actions and sensations, potentially through the mirror mechanism. © 2016 The Author(s).

  19. Developing SyrinOX total antioxidant capacity assay for measuring antioxidants in humans.

    Science.gov (United States)

    Prasetyo, Endry N; Knes, Otto; Nyanhongo, Gibson S; Guebitz, Georg M

    2013-02-01

    Accurate monitoring of the antioxidant status or of oxidative stress in patients is still a big challenge in clinical laboratories. This study investigates the possibility of applying a newly developed total antioxidant capacity assay method based on laccase or peroxidase oxidized syringaldazine [Tetramethoxy azobismethylene quinone (TMAMQ)] which is referred to here as SyrinOX, as a diagnostic tool for monitoring both oxidative stress and antioxidant status in patients. Attempts to adapt the Randox total antioxidant procedure [simultaneous incubation of the radical generating system (metmyoglobin and H(2) O(2) ) and antioxidant sample] for SyrinOX were abandoned after it was discovered that the H(2) O(2) reacted with enzymatically generated TMAMQ and ABTS radicals at a rate of 6.4 × 10(-2) /μM/s and 5.7 × 10(-3) /μM/s respectively. Thus this study for the first time demonstrates the negative effects of H(2) O(2) in the Randox system. This leads to erroneous results because the total antioxidant values obtained are the sum of radicals reduced by antioxidants plus those reacting with the radical generating system. Therefore they should be avoided not only for this particular method but also when using other similar methods. Consequently, SyrinOX is best applied using a three-step approach involving, production of TMAMQ, recovery and purification (free from enzyme and other impurities) and then using TMAMQ for measuring the total antioxidant capacity of samples. Using this approach, the reaction conditions for application of SyrinOX when measuring the total antioxidant capacity of plasma sample were determined to be 50% (v/v) ethanol/50 mM sodium succinate buffer pH 5.5, between 20 and 25 °C for at least 1 h. © 2012 The Authors. International Journal of Experimental Pathology © 2012 International Journal of Experimental Pathology.

  20. Plasticity in mitochondrial cristae density allows metabolic capacity modulation in human skeletal muscle

    DEFF Research Database (Denmark)

    Nielsen, Joachim; Gejl, Kasper D; Hey-Mogensen, Martin

    2017-01-01

    experimental studies have shown that respiration per mitochondria varies.Modelling studies have hypothesised that this variation in respiration per mitochondria depends on plasticity in cristae density, but currently evidence for such a mechanism is lacking. Here, we confirm this hypothesis by showing that...... that this mechanism allows evasion of the trade-off between cell occupancy by mitochondria and other cellular constituents and improved metabolic capacity and fuel catabolism during prolonged elevated energy requirements. This article is protected by copyright. All rights reserved....

  1. Influence of two high-intensity intermittent training programmes on anaerobic capacity in humans

    Directory of Open Access Journals (Sweden)

    K Buśko

    2011-03-01

    Full Text Available The aim of this work was to determine the influence of a maximal effort training programme with variation of the pedalling rate on the anaerobic capacity, post-exercise maximal concentration of lactic acid (LA and acid-alkali balance changes in the capillary blood. Identification of the dependence between the lactic acid concentration and the anaerobic capacity produced in the Wingate test was also the goal. The hypothesis that cycloergometer training consisting of maximal efforts with a load equal to 10% of body weight (BW will elicit bigger changes of measured values in comparison to training with a load of 5% BW was verified. Twenty non-athletes, students of UPE took part in the study. They were divided into 2 groups. The first group (M10; n=9 performed maximal efforts with a load equal to 10% of body weight (BW. The second group (M5; n=11 performed maximal efforts with a load of 5% BW. Control measurements of anaerobic capacity (Wingate test were taken every Monday: before the test (0, during 4 weeks of training (1-4 and for 2 weeks after the test (5-6. Blood for the determination of lactic acid concentration and acid-alkali balance was taken from the fingertip before performing the Wingate test – in the 5th, 7th, 9th and 30th resting minute. Changes of maximal power (Pmax were not statistically significant in either group. Significant differences were found between the two groups after the second week of rest. For mean power (Pm the most important changes were noted in the first week from training (M10 – 6.5%; M5 – 11.0% No significant differences were found between groups. Average values of the individual LA concentration peak (the highest LA concentration occurring after the Wingate test for each individual changed significantly in group M10 from 15.233±2.367 mmol/l in the measurement made before training to 12.340±2.353 mmol/l in the measurement taken 2 weeks after training. Group M5 is characterised by a change of this factor

  2. Immune response capacity after human splenic autotransplantation - Restoration of response to individual pneumococcal vaccine subtypes

    NARCIS (Netherlands)

    Leemans, R; Manson, W; Snijder, JAM; Smit, JW; Klasen, HJ; The, TH; Timens, W

    Objective To evaluate features of general immune function, in particular the restoration of the humoral immune response to pneumococcal capsular polysaccharides, in humans undergoing a spleen autotransplantation after splenectomy because of trauma. Summary Background Data After splenectomy, patients

  3. Radiosensitivity and repair capacity of two xenografted human soft tissue sarcomas to photons and fast neutrons

    International Nuclear Information System (INIS)

    Budach, V.; Stuschke, M.; Budach, W.; Krause, U.; Streffer, C.; Sack, H.

    1989-01-01

    The radiation response, the relative biological effectiveness (RBE) and sublethal damage repair of two xenografted human soft tissue sarcomas after single doses and fractionated irradiation with 60 Co and 5.8 MeV fast neutrons are presented. (author)

  4. Characterisation of human skin models - stability, metabolic capacity and comparative investigations in percutaneous absorption

    OpenAIRE

    Schreiber, Sylvia

    2010-01-01

    In recent years, the demand for alternative test methods in safety assessment of cosmetics, risk assessment of chemicals, and testing of pharmaceuticals was increasingly included in the EU directives. Thereby, alternative test methods for the determination of percutaneous absorption should achieve a more reliable in vivo prediction of the response of human skin than animal skin. Even though freshly excised human skin is considered as a preferred test matrix its routine use is often difficult ...

  5. WNT Inhibitory Activity of Malus Pumila miller cv Annurca and Malus domestica cv Limoncella Apple Extracts on Human Colon-Rectal Cells Carrying Familial Adenomatous Polyposis Mutations.

    Science.gov (United States)

    Riccio, Gennaro; Maisto, Maria; Bottone, Sara; Badolati, Nadia; Rossi, Giovanni Battista; Tenore, Gian Carlo; Stornaiuolo, Mariano; Novellino, Ettore

    2017-11-18

    Inhibitors of the Wingless-related Integration site (WNT)/β-catenin pathway have recently been under consideration as potential chemopreventive agents against Familial Adenomatous Polyposis (FAP). This autosomal-dominant syndrome is caused by germline mutations in the gene coding for the protein APC and leads to hyperactivation of the WNT/β-catenin signaling pathway, uncontrolled intestinal cell proliferation and formation of adenocarcinomas. The aim of the present work was to: (i) test, on in vitro cultures of cells carrying FAP mutations and on ex vivo biopsies of FAP patients, the WNT inhibitory activity of extracts from two common southern Italian apples, Malus pumila Miller cv. 'Annurca' and Malus domestica cv 'Limoncella'; (ii) identify the mechanisms underpinning their activities and; (iii) evaluate their potency upon gastrointestinal digestion. We here show that both Annurca and Limoncella apple extracts act as WNT inhibitors, mostly thanks to their polyphenolic contents. They inhibit the pathway in colon cells carrying FAP mutations with active dilutions falling in ranges close to consumer-relevant concentrations. Food-grade manufacturing of apple extracts increases their WNT inhibitory activity as result of the conversion of quercetin glycosides into the aglycone quercetin, a potent WNT inhibitor absent in the fresh fruit extract. However, in vitro simulated gastrointestinal digestion severely affected WNT inhibitory activity of apple extracts, as result of a loss of polyphenols. In conclusion, our results show that apple extracts inhibit the WNT pathway in colon cells carrying FAP mutations and represent a potential nutraceutical alternative for the treatment of this pathology. Enteric coating is advisable to preserve the activity of the extracts in the colon-rectal section of the digestive tract.

  6. WNT Inhibitory Activity of Malus Pumila miller cv Annurca and Malus domestica cv Limoncella Apple Extracts on Human Colon-Rectal Cells Carrying Familial Adenomatous Polyposis Mutations

    Directory of Open Access Journals (Sweden)

    Gennaro Riccio

    2017-11-01

    Full Text Available Inhibitors of the Wingless-related Integration site (WNT/β-catenin pathway have recently been under consideration as potential chemopreventive agents against Familial Adenomatous Polyposis (FAP. This autosomal-dominant syndrome is caused by germline mutations in the gene coding for the protein APC and leads to hyperactivation of the WNT/β-catenin signaling pathway, uncontrolled intestinal cell proliferation and formation of adenocarcinomas. The aim of the present work was to: (i test, on in vitro cultures of cells carrying FAP mutations and on ex vivo biopsies of FAP patients, the WNT inhibitory activity of extracts from two common southern Italian apples, Malus pumila Miller cv. ‘Annurca’ and Malus domestica cv ‘Limoncella’; (ii identify the mechanisms underpinning their activities and; (iii evaluate their potency upon gastrointestinal digestion. We here show that both Annurca and Limoncella apple extracts act as WNT inhibitors, mostly thanks to their polyphenolic contents. They inhibit the pathway in colon cells carrying FAP mutations with active dilutions falling in ranges close to consumer-relevant concentrations. Food-grade manufacturing of apple extracts increases their WNT inhibitory activity as result of the conversion of quercetin glycosides into the aglycone quercetin, a potent WNT inhibitor absent in the fresh fruit extract. However, in vitro simulated gastrointestinal digestion severely affected WNT inhibitory activity of apple extracts, as result of a loss of polyphenols. In conclusion, our results show that apple extracts inhibit the WNT pathway in colon cells carrying FAP mutations and represent a potential nutraceutical alternative for the treatment of this pathology. Enteric coating is advisable to preserve the activity of the extracts in the colon-rectal section of the digestive tract.

  7. Carrying on the Good Fight: Summary Paper from Think Tank 2000--Advancing the Civil and Human Rights of People with Disabilities from Diverse Cultures.

    Science.gov (United States)

    National Council on Disability, Washington, DC.

    This paper summarizes a May 2000 conference about advancing the civil and human rights of people with disabilities from diverse cultures. The conference included people with disabilities from diverse cultures and members of national civil rights organizations. The conference identified five priority areas for attention: (1) cultivating leadership…

  8. 公共资源类旅游景区水环境承载力研究——以武汉市东湖风景区为例%Carrying capacity of water environment in public tourism resources: A case of East Lake scenic area in Wuhan

    Institute of Scientific and Technical Information of China (English)

    黄璨; 邓宏兵; 李小帆; 李会琴

    2013-01-01

    The water environment in public tourism resources is affected by the socio-economic system and travel system.This paper builds a multi-target model of water environment carrying capacity by selecting an index system that consists of population,GDP,irrigated area,number of tourists,number of guesthouse day reception,total nitrogen (TP),chemical oxygen demand (COD) and biochemical oxygen demand (BOD).Shuiguohu Lake,Tanglinghu Lake and Guozhenghu Lake are selected as the sub-lakes of the east lake area to evaluate the water environmental carrying capacity,by adopting the Analytic Hierarchy Process (AHP) to determine the weight of indexes on water environmental carrying capacity of the East Lake scenic area.This paper also uses a typical model to calculate water environmental carrying capacity of East Lake scenic area in 2011.The results show that the irrigation area and GDP have exceeded the carrying standards,while population and tourism indicators are within the carrying capacity.All the indicators of water quality have exceeded the carrying standards.The scores for water environmental carrying capacity of Shuiguohu,Tanglinghu and Guozhenghu lake are 4.03,3.86 and 3.95 respectively,which are greatly overloaded.%公共资源类旅游景区水环境受到社会经济系统和旅游系统两方面影响,本文通过选取人口、GDP、灌溉面积、游客量、宾馆日接待人数、TP、COD、BOD等指标,构建水环境承载力多目标模型.同时,选取水果湖、汤菱湖和郭郑湖3个子湖对东湖风景区水环境承载力进行评价,并运用层次分析法(AHP)确定各指标对东湖风景区水环境承载力的权重,采用典型水环境承载力概念模型计算了2011年武汉市东湖风景区水环境承载力.结果表明,东湖风景区水资源承载力表现为灌溉面积和GDP承载度超标,而人口和旅游指标承载度未达到饱和,水质承载力各指标的承载度超标.水果湖、汤菱湖和郭郑湖的水

  9. Sortilin-Related Receptor Expression in Human Neural Stem Cells Derived from Alzheimer’s Disease Patients Carrying the APOE Epsilon 4 Allele

    Directory of Open Access Journals (Sweden)

    Alen Zollo

    2017-01-01

    Full Text Available Alzheimer’s disease (AD is the most common form of dementia in the elderly; important risk factors are old age and inheritance of the apolipoprotein E4 (APOE4 allele. Changes in amyloid precursor protein (APP binding, trafficking, and sorting may be important AD causative factors. Secretase-mediated APP cleavage produces neurotoxic amyloid-beta (Aβ peptides, which form lethal deposits in the brain. In vivo and in vitro studies have implicated sortilin-related receptor (SORL1 as an important factor in APP trafficking and processing. Recent in vitro evidence has associated the APOE4 allele and alterations in the SORL1 pathway with AD development and progression. Here, we analyzed SORL1 expression in neural stem cells (NSCs from AD patients carrying null, one, or two copies of the APOE4 allele. We show reduced SORL1 expression only in NSCs of a patient carrying two copies of APOE4 allele with increased Aβ/SORL1 localization along the degenerated neurites. Interestingly, SORL1 binding to APP was largely compromised; this could be almost completely reversed by γ-secretase (but not β-secretase inhibitor treatment. These findings may yield new insights into the complex interplay of SORL1 and AD pathology and point to NSCs as a valuable tool to address unsolved AD-related questions in vitro.

  10. Combined short-arm centrifuge and aerobic exercise training improves cardiovascular function and physical working capacity in humans.

    Science.gov (United States)

    Yang, Chang-Bin; Zhang, Shu; Zhang, Yu; Wang, Bing; Yao, Yong-Jie; Wang, Yong-Chun; Wu, Yan-Hong; Liang, Wen-Bin; Sun, Xi-Qing

    2010-12-01

    Musculoskeletal and cardiovascular deconditioning occurring in long-term spaceflight gives rise to the needs to develop new strategies to counteract these adverse effects. Short-arm centrifuge combined with ergometer has been proposed as a strategy to counteract adverse effects of microgravity. This study sought to investigate whether the combination of short-arm centrifuge and aerobic exercise training have advantages over short-arm centrifuge or aerobic exercise training alone. One week training was conducted by 24 healthy men. They were randomly divided into 3 groups: (1) short-arm centrifuge training, (2) aerobic exercise training, 40 W, and (3) combined short-arm centrifuge and aerobic exercise training. Before and after training, the cardiac pump function represented by stroke volume, cardiac output, left ventricular ejection time, and total peripheral resistance was evaluated. Variability of heart rate and systolic blood pressure were determined by spectral analysis. Physical working capacity was surveyed by near maximal physical working capacity test. The 1-week combined short-arm centrifuge and aerobic exercise training remarkably ameliorated the cardiac pump function and enhanced vasomotor sympathetic nerve modulation and improved physical working capacity by 10.9% (Pcentrifuge nor the aerobic exercise group showed improvements in these functions. These results demonstrate that combined short-arm centrifuge and aerobic exercise training has advantages over short-arm centrifuge or aerobic exercise training alone in influencing several physiologically important cardiovascular functions in humans. The combination of short-arm centrifuge and aerobic exercise offers a promising countermeasure to microgravity.

  11. In vitro induction of alkaline phosphatase levels predicts in vivo bone forming capacity of human bone marrow stromal cells

    Directory of Open Access Journals (Sweden)

    Henk-Jan Prins

    2014-03-01

    Full Text Available One of the applications of bone marrow stromal cells (BMSCs that are produced by ex vivo expansion is for use in in vivo bone tissue engineering. Cultured stromal cells are a mixture of cells at different stages of commitment and expansion capability, leading to a heterogeneous cell population that each time can differ in the potential to form in vivo bone. A parameter that predicts for in vivo bone forming capacity is thus far lacking. We employed single colony-derived BMSC cultures to identify such predictive parameters. Using limiting dilution, we have produced sixteen single CFU-F derived BMSC cultures from human bone marrow and found that only five of these formed bone in vivo. The single colony-derived BMSC strains were tested for proliferation, osteogenic-, adipogenic- and chondrogenic differentiation capacity and the expression of a variety of associated markers. The only robust predictors of in vivo bone forming capacity were the induction of alkaline phosphatase, (ALP mRNA levels and ALP activity during in vitro osteogenic differentiation. The predictive value of in vitro ALP induction was confirmed by analyzing “bulk-cultured” BMSCs from various bone marrow biopsies. Our findings show that in BMSCs, the additional increase in ALP levels over basal levels during in vitro osteogenic differentiation is predictive of in vivo performance.

  12. Normal mitochondrial function and increased fat oxidation capacity in leg and arm muscles in obese humans

    DEFF Research Database (Denmark)

    Ara, I; Larsen, S; Stallknecht, Bente Merete

    2011-01-01

    was that fat oxidation during exercise might be differentially preserved in leg and arm muscles after weight loss.Methods:Indirect calorimetry was used to calculate fat and carbohydrate oxidation during both progressive arm-cranking and leg-cycling exercises. Muscle biopsy samples were obtained from musculus...... deltoideus (m. deltoideus) and m. vastus lateralis muscles. Fibre-type composition, enzyme activity and O(2) flux capacity of saponin-permeabilized muscle fibres were measured, the latter by high-resolution respirometry.Results:During the graded exercise tests, peak fat oxidation during leg cycling...... and the relative workload at which it occurred (FatMax) were higher in PO and O than in C. During arm cranking, peak fat oxidation was higher in O than in C, and FatMax was higher in O than in PO and C. Similar fibre-type composition was found between groups. Plasma adiponectin was higher in PO than in C and O...

  13. Human Capacity Building in Energy Efficiency and Renewable Energy System Maintenance for the Yurok Tribe

    Energy Technology Data Exchange (ETDEWEB)

    Engel, R. A.' Zoellick, J J.

    2007-07-31

    From July 2005 to July 2007, the Schatz Energy Research Center (SERC) assisted the Yurok Tribe in the implementation of a program designed to build the Tribe’s own capacity to improve energy efficiency and maintain and repair renewable energy systems in Tribal homes on the Yurok Reservation. Funding for this effort was provided by the U.S. Department of Energy’s Tribal Program under First Steps grant award #DE-FG36-05GO15166. The program’s centerpiece was a house-by-house needs assessment, in which Tribal staff visited and conducted energy audits at over fifty homes. The visits included assessment of household energy efficiency and condition of existing renewable energy systems. Staff also provided energy education to residents, evaluated potential sites for new household renewable energy systems, and performed minor repairs as needed on renewable energy systems.

  14. Generation of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene.

    Science.gov (United States)

    Liu, Senquan; Ye, Zhaohui; Gao, Yongxing; He, Chaoxia; Williams, Donna W; Moliterno, Alison; Spivak, Jerry; Huang, He; Cheng, Linzhao

    2017-01-01

    Activating point mutations in the MPL gene encoding the thrombopoietin receptor are found in 3%-10% of essential thrombocythemia (ET) and myelofibrosis patients. Here, we report the derivation of induced pluripotent stem cells (iPSCs) from an ET patient with a heterozygous MPL V501L mutation. Peripheral blood CD34 + progenitor cells were reprogrammed by transient plasmid expression of OCT4, SOX2, KLF4, c-MYC plus BCL2L1 (BCL-xL) genes. The derived line M494 carries a MPL V501L mutation, displays typical iPSC morphology and characteristics, are pluripotent and karyotypically normal. Upon differentiation, the iPSCs are able to differentiate into cells derived from three germ layers. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  15. Characterization of IncN plasmids carrying blaCTX-M-1 and qnr genes in Escherichia coli and Salmonella from animals, the environment and humans

    DEFF Research Database (Denmark)

    Dolejska, Monika; Villa, Laura; Hasman, Henrik

    2013-01-01

    were compared using restriction fragment length polymorphism (RFLP), plasmid multilocus sequence typing (pMLST) and hybridization with repN, qnrS1, qnrB19 or blaCTX-M-1 probes. Plasmids pKT58A and pHHA45 were sequenced using the 454-Genome Sequencer FLX platform on a library constructed from plasmid...... DNA purified from the respective E. coli transformants.Results Three types of IncN plasmids carrying blaCTX-M-1, qnrS1 and qnrB19 genes were identified in strains isolated from the Czech Republic, Poland, Slovakia, Denmark, Italy and the Netherlands, corresponding to pMLST sequence type (ST) 1, ST3...

  16. Using molecular classification to predict gains in maximal aerobic capacity following endurance exercise training in humans

    DEFF Research Database (Denmark)

    Timmons, James A; Knudsen, Steen; Rankinen, Tuomo

    2010-01-01

    A low maximal oxygen consumption (VO2max) is a strong risk factor for premature mortality. Supervised endurance exercise training increases VO2max with a very wide range of effectiveness in humans. Discovering the DNA variants that contribute to this heterogeneity typically requires substantial s...

  17. 基于水环境承载力评价的产业选择——以扬州市北部沿湖地区为例%Selecting industries based on evaluation of the water environment's carrying capacity: a case study of the Northern Lakeside Region of Yangzhou City

    Institute of Scientific and Technical Information of China (English)

    张姗姗; 张落成; 董雅文; 杨永可

    2017-01-01

    Natural and social conditions play a considerable role in regional industrial development and distribution.The water environment's carrying capacity,a major natural factor,has a direct effect on the development of regional industry,particularly in regions with dense water networks.The northern lakeside region of Yangzhou City,which belongs to both the Yangtze and Huaihe basins,was taken as a typical case to discuss the relationship between the water environment's carrying capacity and industrial development.First,we divided the study area into a series of ecological units,500 m × 500 m in size.Second,three indexes (including the drainage component index,water conservancy facilities index,and discrepancy of water quality index) were generated to evaluate the water environment's carrying capacity for each ecological unit through the "multi-factor comprehensive analysis" method.Then,the carrying capacity was classified into five levels,namely:"highest","higher","general","lower",and "lowest",which accounted for 0.38%,21.74%,29.67%,13.65%,and 34.56% of the total area,respectively.Considering the spatial difference of the water environment's carrying capacity and the overall deployment of the national economy,it is appropriate to develop modem ecological agriculture and lake tourism in the study area.These industries are regarded as environmentally friendly or ecological conservation-oriented industries,which belong to the category of green industry.On the one hand,modem ecological agriculture is an important choice for the transformation and development of traditional agriculture.On the other hand,developing a lake-tourism industry is the best way to take full advantage of the rich wetland resources,lake landscape,and modem water conservancy projects.In particular,areas of the Shaobo Lake and inflow rivers further downstream with "higher" and "highest" carrying capacity are suitable for the ecological breeding of special aquatic fauna.Further,the lakeshore

  18. A general native-state method for determination of proliferation capacity of human normal and tumor tissues in vitro

    International Nuclear Information System (INIS)

    Hoffman, R.M.; Connors, K.M.; Meerson-Monosov, A.Z.; Herrera, H.; Price, J.H.

    1989-01-01

    An important need in cancer research and treatment is a physiological means in vitro by which to assess the proliferation capacity of human tumors and corresponding normal tissue for comparison. The authors have recently developed a native-state, three-dimensional, gel-supported primary culture system that allows every type of human cancer to grow in vitro at more than 90% frequency, with maintenance of tissue architecture, tumor-stromal interaction, and differentiated functions. Here they demonstrate that the native-state culture system allows proliferation indices to be determined for all solid cancer types explanted directly from surgery into long-term culture. Normal tissues also proliferate readily in this system. The degree of resolution of measurement of cell proliferation by histological autoradiography within the cultured tissues is greatly enhanced with the use of epi-illumination polarization microscopy. The histological status of the cultured tissues can be assessed simultaneously with the proliferation status. Carcinomas generally have areas of high epithelial proliferation with quiescent stromal cells. Sarcomas have high proliferation of cells of mesenchymal organ. Normal tissues can also proliferate at high rates. An image analysis system has been developed to automate proliferation determination. The high-resolution physiological means described here to measure the proliferation capacity of tissues will be important in further understanding of the deregulation of cell proliferation in cancer as well as in cancer prognosis and treatment

  19. 滨海旅游环境承载力评价与量化测度研究——以山东半岛蓝色经济区为例%Evaluation and Quantitative Measurement on Coastal Tourism Environmental Carrying Capacity: A Case Study on the Blue Shandong Peninsula Economic Zone

    Institute of Scientific and Technical Information of China (English)

    刘佳; 于水仙; 王佳

    2012-01-01

    旅游环境承载力是区域旅游可持续发展的重要评判依据,目前国内外旅游环境承载力研究方法和技术手段仍显不足,多以静态评价分析为主,缺乏动态预测研究,旅游环境承载力定量研究的技术与方法尚需深入探索.本文结合滨海旗游的自身特点,构建由资源、生态、经济、社会四个承载子系统构成的滨海旅游环境承载力评价体系,按照评价体系的层次结构构造判断矩阵,采用层次分析法确定评价指标权重,运用物元评价模型和灰色预测模型,对山东半岛蓝色经济区滨海旅游环境承载力水平进行量化测度与系统分析.结果表明,半岛蓝色经济区滨海旅游环境各个承载子系统存在一定的承载差异,滨海旅游资源和生态环境承载能力较强,滨海旅游经济和社会环境子系统还有较大的承载空间;从时序变化上看,滨海旅游环境综合承载能为不断提高,并呈现可持续的运行态势;从空间差异上着,青岛、烟台、潍坊和威海四个城市滨海旅游环境承载状态处于“良好”等级,而东营、日照和滨州三个城市滨海旅游环境承载状态处于“一般”等级.因此,提高区域整体承载潜力,平衡地区承载差异,对于促进半岛蓝色经济区滨海旅游资源有序开发和滨海旅游产业合理布局具有重要的现实意义.%Tourism environmental carrying capacity is the important evaluation basis of regional tourism sustainable development; however, relative researches on its methods and techniques are still inadequate at home and abroad. Those studies are mostly based on static evaluation analysis and lack of dynamic prediction. So the techniques and methods of quantitative research on tourism environmental carrying capacity still need to be explored in depth timely. In accordance with own characteristics of the coastal tourism, the paper constructs ihe evaluation system of coastal tourism environmental

  20. Baculovirus-mediated expression and isolation of human ribosomal phosphoprotein P0 carrying a GST-tag in a functional state

    International Nuclear Information System (INIS)

    Abo, Yohichi; Hagiya, Akiko; Naganuma, Takao; Tohkairin, Yukiko; Shiomi, Kunihiro; Kajiura, Zenta; Hachimori, Akira; Uchiumi, Toshio; Nakagaki, Masao

    2004-01-01

    We constructed an overexpression system for human ribosomal phosphoprotein P0, together with P1 and P2, which is crucially important for translation. Genes for these proteins, fused with the glutathione S-transferase (GST)-tag at the N-terminus, were inserted into baculovirus and introduced to insect cells. The fusion proteins, but not the proteins without the tag, were efficiently expressed into cells as soluble forms. The fusion protein GST.P0 as well as GST.P1/GST.P2 was phosphorylated in cells as detected by incorporation of 32 P and reactivity with monoclonal anti-phosphoserine antibody. GST.P0 expressed in insect cells, but not the protein obtained in Escherichia coli, had the ability to form a complex with P1 and P2 proteins and to bind to 28S rRNA. Moreover, the GST.P0-P1-P2 complex participated in high eEF-2-dependent GTPase activity. Baculovirus expression systems appear to provide recombinant human P0 samples that can be used for studies on the structure and function

  1. A novel mouse model carrying a human cytoplasmic dynein mutation shows motor behavior deficits consistent with Charcot-Marie-Tooth type 2O disease.

    Science.gov (United States)

    Sabblah, Thywill T; Nandini, Swaran; Ledray, Aaron P; Pasos, Julio; Calderon, Jami L Conley; Love, Rachal; King, Linda E; King, Stephen J

    2018-01-29

    Charcot-Marie-Tooth disease (CMT) is a peripheral neuromuscular disorder in which axonal degeneration causes progressive loss of motor and sensory nerve function. The loss of motor nerve function leads to distal muscle weakness and atrophy, resulting in gait problems and difficulties with walking, running, and balance. A mutation in the cytoplasmic dynein heavy chain (DHC) gene was discovered to cause an autosomal dominant form of the disease designated Charcot-Marie-Tooth type 2 O disease (CMT2O) in 2011. The mutation is a single amino acid change of histidine into arginine at amino acid 306 (H306R) in DHC. In order to understand the onset and progression of CMT2, we generated a knock-in mouse carrying the corresponding CMT2O mutation (H304R/+). We examined H304R/+ mouse cohorts in a 12-month longitudinal study of grip strength, tail suspension, and rotarod assays. H304R/+ mice displayed distal muscle weakness and loss of motor coordination phenotypes consistent with those of individuals with CMT2. Analysis of the gastrocnemius of H304R/+ male mice showed prominent defects in neuromuscular junction (NMJ) morphology including reduced size, branching, and complexity. Based on these results, the H304R/+ mouse will be an important model for uncovering functions of dynein in complex organisms, especially related to CMT onset and progression.

  2. The effect of manufacturing tolerances on the load carrying capacity ...

    Indian Academy of Sciences (India)

    bearings based on Hertz theory. The nonlinear connector elements are used to model ... Keywords. Slewing bearing; Hertz theory; load distribution; nonlinear connector elements; tolerance. 1. ..... Ngoi B K A, Lim B H and Ang P S 2000 Nexus method for stack analysis of geometric dimensioning and tolerancing problems.

  3. Optimising the allocation of groundwater carrying capacity in a data ...

    African Journals Online (AJOL)

    2009-08-26

    Aug 26, 2009 ... There are, however, no unified and mature approaches to .... Change process of average annual groundwater level for. Observation Well No. ... Under this circumstance, it is rational to consider only the factors of arable land ...

  4. Saldanha Bay, South Africa III: new production and carrying capacity ...

    African Journals Online (AJOL)

    It is estimated that the total annual production of mussels and oysters, respectively, for a 1 000-ha cultivation area is approximately 40 000–53 000 t y–1 (mainly Mytilus galloprovincialis) and 4 600–6 000 t y–1 (Crassotrea gigas). The combined total production figures constitute only 24–31% of the surplus new production.

  5. The logistic model-generated carrying capacities, maximum ...

    African Journals Online (AJOL)

    This paper deals with the derivation of logistic models for cattle, sheep and goats in a commercial ranching system in Machakos District, Kenya, a savannah ecosystem with average annual rainfall of 589.3 ± 159.3mm and an area of 10 117ha. It involves modelling livestock population dynamics as discrete-time logistic ...

  6. The effect of manufacturing tolerances on the load carrying capacity ...

    Indian Academy of Sciences (India)

    diameter bearing with tolerance on ball and raceway will lead to safe design. .... The leading manufacturers of bearing SKF (Svenska Kullagerfabriken) and .... of production will affect the various dimensions in the bearing and their housings.

  7. Projecting the visual carrying capacity of recreation areas

    Science.gov (United States)

    Thomas J. Nieman; Jane L. Futrell

    1979-01-01

    The aesthetic experience of people utilizing the recreational resources of the national parks and forests of the United States is of primary importance since a large percentage of perception is visual. Undesirable intrusions into this sphere of perception substantially reduce the level of enjoyment or satisfaction derived from the recreation experience. Perceived...

  8. ecological carrying capacity of saanane island proposed national

    African Journals Online (AJOL)

    Zoology

    definition developed by Hudak in (1999) which refers ... investment. The population of introduced ... Samples were collected from stratified systematic ... average height were used to estimate the ..... about using optimum numbers of individuals.

  9. Ameloblastin Peptides Modulates the Osteogenic Capacity of Human Mesenchymal Stem Cells

    Czech Academy of Sciences Publication Activity Database

    Stakkestad, O.; Lyngstadaas, S. P.; Vondrášek, Jiří; Gordeladze, J. O.; Reseland, J. E.

    2017-01-01

    Roč. 8, Feb 7 (2017), č. článku 58. ISSN 1664-042X Institutional support: RVO:61388963 Keywords : ameloblastin * biomineralization * bone growth * exon 5 * human mesenchymal stem cells * osteogenesis * proliferation Subject RIV: ED - Physiology OBOR OECD: Physiology (including cytology) Impact factor: 4.134, year: 2016 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293776/pdf/fphys-08-00058.pdf

  10. Antioxidant capacity of Ugni molinae fruit extract on human erythrocytes: an in vitro study.

    Science.gov (United States)

    Suwalsky, Mario; Avello, Marcia

    2014-08-01

    Ugni molinae is an important source of molecules with strong antioxidant activity widely used as a medicinal plant in Southern Chile-Argentina. Total phenol concentration from its fruit extract was 10.64 ± 0.04 mM gallic acid equivalents. Analysis by means of HPLC/MS indicated the presence of the anthocyanins cyanidin and peonidin, and the flavonol quercitin, all in glycosylated forms. Its antioxidant properties were assessed in human erythrocytes in vitro exposed to HClO oxidative stress. Scanning electron microscopy showed that HClO induced an alteration in erythrocytes from a normal shape to echinocytes; however, this change was highly attenuated in samples containing U. molinae extracts. It also had a tendency in order to reduce the hemolytic effect of HClO. In addition, X-ray diffraction experiments were performed in dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine bilayers, classes of lipids preferentially located in the outer and inner monolayers, respectively, of the human erythrocyte membrane. It was observed that U. molinae only interacted with DMPC. Results by fluorescence spectroscopy on DMPC large unilamellar vesicles and isolated unsealed human erythrocyte membranes also showed that it interacted with the erythrocyte membrane and DMPC. It is possible that the location of U. molinae components into the membrane outer monolayer might hinder the diffusion of HClO and of free radicals into cell membranes and the consequent decrease of the kinetics of free radical reactions.

  11. Plasma membrane Ca2+-ATPase 4: interaction with constitutive nitric oxide synthases in human sperm and prostasomes which carry Ca2+/CaM-dependent serine kinase.

    Science.gov (United States)

    Andrews, Rachel E; Galileo, Deni S; Martin-DeLeon, Patricia A

    2015-11-01

    Deletion of the gene encoding the widely conserved plasma membrane calcium ATPase 4 (PMCA4), a major Ca(2+) efflux pump, leads to loss of sperm motility and male infertility in mice. PMCA4's partners in sperm and how its absence exerts its effect on fertility are unknown. We hypothesize that in sperm PMCA4 interacts with endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) which are rapidly activated by Ca(2+), and that these fertility-modulating proteins are present in prostasomes, which deliver them to sperm. We show that in human sperm PMCA4 is present on the acrosome, inner acrosomal membrane, posterior head, neck, midpiece and the proximal principal piece. PMCA4 localization showed inter- and intra-individual variation and was most abundant at the posterior head/neck junction, co-localizing with NOSs. Co-immunoprecipitations (Co-IP) revealed a close association of PMCA4 and the NOSs in Ca(2+) ionophore-treated sperm but much less so in uncapacitated untreated sperm. Fluorescence resonance energy transfer (FRET) showed a similar Ca(2+)-related association: PMCA4 and the NOSs are within 10 nm apart, and preferentially so in capacitated, compared with uncapacitated, sperm. FRET efficiencies varied, being significantly (P < 0.001) higher at high cytosolic Ca(2+) concentration ([Ca(2+)]c) in capacitated sperm than at low [Ca(2+)]c in uncapacitated sperm for the PMCA4-eNOS complex. These dynamic interactions were not seen for PMCA4-nNOS complexes, which had the highest FRET efficiencies. Further, along with Ca(2+)/CaM-dependent serine kinase (CASK), PMCA4 and the NOSs are present in the seminal plasma, specifically in prostasomes where Co-IP showed complexes similar to those in sperm. Finally, flow cytometry demonstrated that following co-incubation of sperm and seminal plasma, PMCA4 and the NOSs can be delivered in vitro to sperm via prostasomes. Our findings indicate that PMCA4 interacts simultaneously with the NOSs preferentially at

  12. Adaptive capacity and human cognition: the process of individual adaptation to climate change

    Energy Technology Data Exchange (ETDEWEB)

    Grothmann, T. [Potsdam Institute for Climate Impact Research, Potsdam (Germany). Department of Global Change and Social Systems; Patt, A. [Boston University (United States). Department of Geography

    2005-10-01

    Adaptation has emerged as an important area of research and assessment among climate change scientists. Most scholarly work has identified resource constraints as being the most significant determinants of adaptation. However, empirical research on adaptation has so far mostly not addressed the importance of measurable and alterable psychological factors in determining adaptation. Drawing from the literature in psychology and behavioural economics, we develop a socio-cognitive Model of Private Proactive Adaptation to Climate Change (MPPACC). MPPACC separates out the psychological steps to taking action in response to perception, and allows one to see where the most important bottlenecks occur - including risk perception and perceived adaptive capacity, a factor largely neglected in previous climate change research. We then examine two case studies - one from urban Germany and one from rural Zimbabwe - to explore the validity of MPPACC to explaining adaptation. In the German study, we find that MPPACC provides better statistical power than traditional socio-economic models. In the Zimbabwean case study, we find a qualitative match between MPPACC and adaptive behaviour. Finally, we discuss the important implications of our findings both on vulnerability and adaptation assessments, and on efforts to promote adaptation through outside intervention. (author)

  13. Caveolin-1 as a novel indicator of wound-healing capacity in aged human corneal epithelium.

    Science.gov (United States)

    Rhim, Ji Heon; Kim, Jae Hoon; Yeo, Eui-Ju; Kim, Jae Chan; Park, Sang Chul

    2010-01-01

    Excess caveolin-1 has been reported to play a role in age-dependent hyporesponsiveness to growth factors in vitro. Therefore, we hypothesized that caveolin-1-dependent hyporesponsiveness to growth factors in aged corneal epithelial cells might be responsible for delayed wound healing in vivo. To test this hypothesis, we evaluated corneal wound-healing time by vital staining using fluorescein after laser epithelial keratomileusis (LASEK). We compared wound-healing times in young, middle-aged and elderly patients. We also examined caveolin-1 levels and other aging markers, such as p53 and p21, in the corneal epithelium. Elderly patients generally had higher caveolin-1 levels in the corneal epithelia than young patients. There were, however, variations among individuals with increased caveolin-1 in some young patients and decreased levels in some elderly patients. Wound-healing time after LASEK correlated well with the corneal caveolin-1 status. Therefore, we suggest that caveolin-1 status might be responsible for delayed wound healing in elderly patients after LASEK. Caveolin-1 status might be a regulator for wound-healing capacity and a novel target for in vivo adjustment.

  14. 基于智慧旅游视角的旅游景区承载力研究综述与启示∗%Literature Review and Implications on Tourism Environmental Carrying Capacity Based on the Perspective of Smart Tourism

    Institute of Scientific and Technical Information of China (English)

    袁箐; 陈楠; 程川

    2016-01-01

    随着大数据时代的到来和互联网平台技术的广泛应用,旅游信息化建设成为中国旅游产业可持续发展的重要步骤:智慧旅游应时而生。然而,一些基于景区承载力问题发生的公共旅游安全事故凸显出智慧旅游建设的不足。立足于智慧旅游、智慧景区理论,根据旅游景区承载力理论研究进展的智慧性趋势,将旅游景区承载力分为筑基期、发展期与成熟期3个发展时期,回顾了1980-2015年间国内外承载力的相关研究,并分析每个时期中承载力理论与智慧旅游、智慧景区间的关系;接着通过总结三者间的关系框架图、共融性特征描述了承载力与智慧旅游结合的联系与必然性。最后,针对国内景区承载力研究的智慧化发展进行反思,并针对相关问题提出对策,为后续研究提供理论依据。%With the advent of the era of big data and the wide application of the Internet platform technology,building tourism in-formation has become an important step in the sustainable development of our country's tourism industry.However,some of the safety accidents based on the shortages of carrying capacity revealed the building problems of smarter tourism.Based on the theo-ry of smart tourism,tourist attractions and the smart trend of tourism carrying capacity,this paper divided TCC into 3 periods of base,development and mature.This paper reviewed the development of the carrying capacity concept between 1980-2015 in do-mestic and abroad.It also analyzed the relationship between the theory of smart tourism,tourist attractions and tourism carrying capacity in each period;then,through summarizing the framework of relationship between the three blend features,it described the tourism attractions carrying capacity and smart tourism with contact and necessity.Finally,this paper reflected on the smarter trend of the development of the domestic tourism attractions carrying capacity,and put

  15. Dorsolateral Prefrontal Cortex GABA Concentration in Humans Predicts Working Memory Load Processing Capacity.

    Science.gov (United States)

    Yoon, Jong H; Grandelis, Anthony; Maddock, Richard J

    2016-11-16

    The discovery of neural mechanisms of working memory (WM) would significantly enhance our understanding of complex human behaviors and guide treatment development for WM-related impairments found in neuropsychiatric conditions and aging. Although the dorsolateral prefrontal cortex (DLPFC) has long been considered critical for WM, we still know little about the neural elements and pathways within the DLPFC that support WM in humans. In this study, we tested whether an individual's DLPFC gamma-aminobutryic acid (GABA) content predicts individual differences in WM task performance using a novel behavioral approach. Twenty-three healthy adults completed a task that measured the unique contribution of major WM components (memory load, maintenance, and distraction resistance) to performance. This was done to address the possibility that components have differing GABA dependencies and the failure to parse WM into components would lead to missing true associations with GABA. The subjects then had their DLPFC GABA content measured by single-voxel proton magnetic spectroscopy. We found that individuals with lower DLPFC GABA showed greater performance degradation with higher load, accounting for 31% of variance, p (corrected) = 0.015. This relationship was component, neurochemical, and brain region specific. DLPFC GABA content did not predict performance sensitivity to other components tested; DLPFC glutamate + glutamine and visual cortical GABA content did not predict load sensitivity. These results confirm the involvement of DLPFC GABA in WM load processing in humans and implicate factors controlling DLPFC GABA content in the neural mechanisms of WM and its impairments. This study demonstrated for the first time that the amount of gamma-aminobutryic acid (GABA), the major inhibitory neurotransmitter of the brain, in an individual's prefrontal cortex predicts working memory (WM) task performance. Given that WM is required for many of the most characteristic cognitive and

  16. Genotypic evaluation of etravirine sensitivity of clinical human immunodeficiency virus type 1 (HIV-1) isolates carrying resistance mutations to nevirapine and efavirenz.

    Science.gov (United States)

    Oumar, A A; Jnaoui, K; Kabamba-Mukadi, B; Yombi, J C; Vandercam, B; Goubau, P; Ruelle, J

    2010-01-01

    Etravirine is a second-generation non-nucleoside reverse transcriptase inhibitor (NNRTI) with a pattern of resistance mutations quite distinct from the current NNRTIs. We collected all routine samples of HIV-1 patients followed in the AIDS reference laboratory of UCLouvain (in 2006 and 2007) carrying resistance-associated mutations to nevirapine (NVP) or efavirenz (EFV). The sensitivity to Etravirine was estimated using three different drug resistance algorithms: ANRS (July 2008), IAS (December 2008) and Stanford (November 2008). We also verified whether the mutations described as resistance mutations are not due to virus polymorphisms by the study of 58 genotypes of NNRTI-naive patients. Sixty one samples harboured resistance to NVP and EFV: 41/61 had at least one resistance mutation to Etravirine according to ANRS-IAS algorithms; 42/61 samples had at least one resistance mutation to Etravirine according to the Stanford algorithm. 48 and 53 cases were fully sensitive to Etravirine according to ANRS-IAS and Stanford algorithms, respectively. Three cases harboured more than three mutations and presented a pattern of high-degree resistance to Etravirine according to ANRS-IAS algorithm, while one case harboured more than three mutations and presented high degree resistance to Etravirine according to the Stanford algorithm. The V1061 and V179D mutations were more frequent in the ARV-naive group than in the NNRTI-experienced one. According to the currently available algorithms, Etravirine can still be used in the majority of patients with virus showing resistance to NVP and/or EFV, if a combination of other active drugs is included.

  17. Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.

    Directory of Open Access Journals (Sweden)

    Teruyo Oishi

    Full Text Available Heterotopic ossification (HO is defined as the formation of ectopic bone in soft tissue outside the skeletal tissue. HO is thought to result from aberrant differentiation of osteogenic progenitors within skeletal muscle. However, the precise origin of HO is still unclear. Skeletal muscle contains two kinds of progenitor cells, myogenic progenitors and mesenchymal progenitors. Myogenic and mesenchymal progenitors in human skeletal muscle can be identified as CD56(+ and PDGFRα(+ cells, respectively. The purpose of this study was to investigate the osteogenic differentiation potential of human skeletal muscle-derived progenitors. Both CD56(+ cells and PDGFRα(+ cells showed comparable osteogenic differentiation potential in vitro. However, in an in vivo ectopic bone formation model, PDGFRα(+ cells formed bone-like tissue and showed successful engraftment, while CD56(+ cells did not form bone-like tissue and did not adapt to an osteogenic environment. Immunohistological analysis of human HO sample revealed that many PDGFRα(+ cells were localized in proximity to ectopic bone formed in skeletal muscle. MicroRNAs (miRNAs are known to regulate many biological processes including osteogenic differentiation. We investigated the participation of miRNAs in the osteogenic differentiation of PDGFRα(+ cells by using microarray. We identified miRNAs that had not been known to be involved in osteogenesis but showed dramatic changes during osteogenic differentiation of PDGFRα(+ cells. Upregulation of miR-146b-5p and -424 and downregulation of miR-7 during osteogenic differentiation of PDGFRα(+ cells were confirmed by quantitative real-time RT-PCR. Inhibition of upregulated miRNAs, miR-146b-5p and -424, resulted in the suppression of osteocyte maturation, suggesting that these two miRNAs have the positive role in the osteogenesis of PDGFRα(+ cells. Our results suggest that PDGFRα(+ cells may be the major source of HO and that the newly identified mi

  18. Human airway eosinophils exhibit preferential reduction in STAT signaling capacity and increased CISH expression.

    Science.gov (United States)

    Burnham, Mandy E; Koziol-White, Cynthia J; Esnault, Stephane; Bates, Mary E; Evans, Michael D; Bertics, Paul J; Denlinger, Loren C

    2013-09-15

    Allergic asthma, a chronic respiratory disorder marked by inflammation and recurrent airflow obstruction, is associated with elevated levels of IL-5 family cytokines and elevated numbers of eosinophils (EOS). IL-5 family cytokines elongate peripheral blood EOS (EOS(PB)) viability, recruit EOS(PB) to the airways, and, at higher concentrations, induce degranulation and reactive oxygen species generation. Although airway EOS (EOS(A)) remain signal ready in that GM-CSF treatment induces degranulation, treatment of EOS(A) with IL-5 family cytokines no longer confers a survival advantage. Because the IL-5 family receptors have common signaling capacity, but are uncoupled from EOS(A) survival, whereas other IL-5 family induced endpoints remain functional, we tested the hypothesis that EOS(A) possess a JAK/STAT-specific regulatory mechanism (because JAK/STAT signaling is critical to EOS survival). We found that IL-5 family-induced STAT3 and STAT5 phosphorylation is attenuated in EOS(A) relative to blood EOS from airway allergen-challenged donors. However, IL-5 family-induced ERK1/2 phosphorylation is not altered between EOS(A) and EOS from airway allergen-challenged donors. These observations suggest EOS(A) possess a regulatory mechanism for suppressing STAT signaling distinct from ERK1/2 activation. Furthermore, we found, in EOS(PB), IL-5 family cytokines induce members of the suppressors of cytokine signaling (SOCS) genes, CISH and SOCS1. Additionally, following allergen challenge, EOS(A) express significantly more CISH and SOCS1 mRNA and CISH protein than EOS(PB) counterparts. In EOS(PB), long-term pretreatment with IL-5 family cytokines, to varying degrees, attenuates IL-5 family-induced STAT5 phosphorylation. These data support a model in which IL-5 family cytokines trigger a selective downregulation mechanism in EOS(A) for JAK/STAT pathways.

  19. Human Airway Eosinophils Exhibit Preferential Reduction in STAT Signaling Capacity and Increased CISH Expression1

    Science.gov (United States)

    Burnham, Mandy E.; Koziol-White, Cynthia J.; Esnault, Stephane; Bates, Mary E.; Evans, Michael D.; Bertics, Paul J.; Denlinger, Loren C.

    2013-01-01

    Allergic asthma, a chronic respiratory disorder marked by inflammation and recurrent airflow obstruction, is associated with elevated levels of Interleukin-5 (IL-5) family cytokines, and elevated numbers of eosinophils (EOS). IL-5 family cytokines elongate peripheral blood EOS (EOSPB) viability, recruit EOSPB to the airways, and at higher concentrations, induce degranulation and reactive oxygen species (ROS) generation. While, EOSA remain signal ready in that GM-CSF treatment induces degranulation, treatment of EOSA with IL-5 family cytokines no longer confers a survival advantage. Since the IL-5 family receptors have common signaling capacity, but are uncoupled from EOSA survival while other IL-5 family induced endpoints remain functional, we tested the hypothesis that EOSA possess a JAK/STAT specific regulatory mechanism (since JAK/STAT signaling is critical to EOS survival). We found that IL-5 family-induced STAT3 and STAT5 phosphorylation is attenuated in EOSA relative to blood EOS from airway allergen-challenged donors (EOSCPB). However, IL-5 family induced ERK1/2 phosphorylation is not altered between EOSA and EOSCPB. These observations suggest EOSA possess a regulatory mechanism for suppressing STAT signaling distinct from ERK1/2 activation. Furthermore, we found, in EOSPB, IL-5 family cytokines induce members of the suppressors of cytokine signaling (SOCS) genes, CISH and SOCS1. Additionally, following allergen challenge, EOSA express significantly more CISH and SOCS1 mRNA and CISH protein than EOSPB counterparts. In EOSPB, long-term pretreatment with IL-5 family cytokines, to varying degrees, attenuates IL-5 family induced STAT5 phosphorylation. These data support a model wherein IL-5 family cytokines trigger a selective down-regulation mechanism in EOSA for JAK/STAT pathways. PMID:23956426

  20. Human factors in the safe operation of nuclear power reactors. Survey of research carried out through the Commission of the European Communities

    International Nuclear Information System (INIS)

    Ancarani, A.; Reijen, G. van; Amendola, A.; Mancini, G.

    1983-01-01

    A survey is made of the study and development of approaches to model operators in routine operation and accident sequences. Particular attention is given to the application of simulators. Simulators as tools to improve safety in nuclear power plant operation can be used in two ways: for training and requalification of operators, and for assistance during routine and abnormal events. Whereas the second application is still in its infancy, training simulators of various degrees of complexity and fidelity are widely used. They range from reduced scope to replica models, or can take the form of modular mini-simulators for studying single parts of the plant. The best reliance on a simulator of any kind will be ensured when the definitions of a method for measuring relevant quantities under well-defined conditions (normal and abnormal) will have been established and agreed upon. Results are also given of a study on human factors in relation to risk management in different electricity production processes. This study derives information from the experience of the staff of power stations and analyses management responsibilities and the functions of operating personnel; both aspects have been put in perspective. (author)

  1. Answers to the questions about food irradiation. Concerning results of animal experiments in the specified integrated research. Data carrying a problem in human health were obtained?

    International Nuclear Information System (INIS)

    Ito, Hitoshi

    2007-01-01

    Experts of Food and Agriculture Organization (FAO)/ International Atomic Energy Agency (IAEA)/ World Health Organization (WHO) committee obtained their conclusion in 1980 that food irradiated with <10 kGy of radiation is safe for human health, which is now globally approved. However, in Japan, there have been still opposite opinions based on the doubt in the title on the safety of irradiated food. In this paper, the author answers those questions as he was a member to arrange the Research in the title for food irradiation. Described are data presentation and explanation about results of toxicity studies of diets added with irradiated materials of: weight reductions in rat ovary by irradiated potato (ip) in chronic studies, and in mouse testicle and ovary of F3 generation from the ancestor mice kept on diet with irradiated onion (io); bone malformation in mice by io; and reduction of body weight gain in female rats by ip and increase of mortality of male rats by ip. These are analyzed on the aspects of radiation dose-response, sustained tendency of results throughout the living period or generation, and apparent abnormality by other factors; and normal variation due to individual difference is pointed out to contribute to these findings. The safety test of irradiated food has been conducted valid not only in animal experiments but also other tests like genotoxicity and analysis of radiation-degraded products. (R.T.)

  2. An investigation on the effects of organizational memory and human capital on innovation and absorptive capacity

    Directory of Open Access Journals (Sweden)

    Zahra Mansouri

    2014-06-01

    Full Text Available Innovation is considered as an enabling tool for creating value and sustainable competitive advantage for organizations in dynamic environment of our times along with its ever-increasing complexities. Until innovation in developing countries needs more capital and talent, human capital (HC is a key factor in innovative performance. People are the most important factor of exploitation of foreign knowledge; and knowledge focus in business environment is highly dependent on external information sources in order to develop innovation and improve performance of the company. Studies have shown that although importance level of knowledge is increased, but only 30 percent of existing knowledge is being used in organizations. Expensive but evitable mistakes take place due to information blockage; and the risk of losing knowledge is exceptionally higher when people leave the organization. This study is among the field research. The population includes large manufacturing firms across Khuzestan Province having been active throughout 2013. The data collection tool was a structured questionnaire and sampling is convenience method. The research questionnaire was distributed among a sample of 331 R&D and Human resources Experts selected using simple random sampling. The collected data were analyzed using SPSS and Amos statistical programs. The results indicate that organizational memory had significant impact on ACAP and innovation. Results also showed a significant positive effect of HC on ACAP, but did not support the effect of HC on innovation.

  3. Experiments on the radioimmunological determination of the triiodothyronine binding capacity of human blood serum

    International Nuclear Information System (INIS)

    Traub, H.W.J.

    1980-01-01

    Bovine serum antibodies have been obtained from rabbits. Constant amounts of antibodies have been incubated with increasing amounts of antigens. The maximum value found for T 3 binding to the antigen-antibody complex was 66 μg RA/mg antigen. Human serum samples containing 0,05 ng 125 J-T 3 /μl were incubated with 600 μg RA, and the antigen was precipitated with 8,4 mg anti-RA. The mean value obtained for 30 enthyreotic test persons was a binding to RA of 7.9 +- 0,52% 125 J-T 3 . Comparative measurements of patient and standard serum samples shared only slight differences. This means that the measured data do not reflect the thyroid function. (orig./MG) [de

  4. An in vitro study on the antioxidant capacity of usnic acid on human erythrocytes and molecular models of its membrane.

    Science.gov (United States)

    Suwalsky, M; Jemiola-Rzeminska, M; Astudillo, C; Gallardo, M J; Staforelli, J P; Villena, F; Strzalka, K

    2015-11-01

    Usnic acid (UA) has been associated with chronic diseases through its antioxidant action. Its main target is the cell membrane; however, its effect on that of human erythrocytes has been scarcely investigated. To gain insight into the molecular mechanisms of the interaction between UA and cell membranes human erythrocytes and molecular models of its membrane have been utilized. Dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE) were chosen as representative of phospholipid classes located in the outer and inner monolayers of the erythrocyte membrane, respectively. Results by X-ray diffraction showed that UA produced structural perturbations on DMPC and DMPE bilayers. DSC studies have indicated that thermotropic behavior of DMPE was most strongly distorted by UA than DMPC, whereas the latter is mainly affected on the pretransition. Scanning electron (SEM) and defocusing microscopy (DM) showed that UA induced alterations to erythrocytes from the normal discoid shape to echinocytes. These results imply that UA molecules were located in the outer monolayer of the erythrocyte membrane. Results of its antioxidant properties showed that UA neutralized the oxidative capacity of HClO on DMPC and DMPE bilayers; SEM, DM and hemolysis assays demonstrated the protective effect of UA against the deleterious oxidant effects of HClO upon human erythrocytes. Copyright © 2015 Elsevier B.V. All rights reserved.

  5. Regional assignment of seven genes on chromosome 1 of man by use of man-Chinese hamster somatic cell hybrids. I. Results obtained after hybridization of human cells carrying reciprocal translocations involving chromosome 1.

    Science.gov (United States)

    Jongsma, A P; Burgerhout, W G

    1977-01-01

    Regional localization studies of genes coding for human PGD, PPH1, PGM1, UGPP, GuK1, Pep-C, and FH, which have been assigned to chromosome 1, were performed with man-Chinese hamster somatic cell hybrids, Informative hybrids that retained fragments of the human chromosome 1 were produced by fusion of hamster cells with human cells carrying reciprocal translocations involving chromosome 1. Analysis of the hybrids that retained one of the translocation chromosomes or de novo rearrangements involving the human 1 revealed the following gene positions: PGD and PPH1 in 1pter leads to 1p32, PGM1 in 1p32 leads to 1p22, UGPP and GuK1 in 1q21 leads to 1q42, FH in 1qter leads to 1q42, and Pep-C probably in 1q42.

  6. Use of neutron beams for fundamental research, applications and human capacity building: From national to regional perspectives

    International Nuclear Information System (INIS)

    Nothnagel, S.G.

    2010-01-01

    The SAFARI-1 research reactor at NECSA South Africa is currently one of the best utilized research reactors in the world. Apart from being used for materials irradiation and isotope production, there is a history of innovative utilization of neutron beam line techniques, such as neutron diffraction (strain scanning, powder and single crystal), neutron radiography/tomography, prompt gamma-ray neutron activation analysis and small angle neutron scattering both for NECSA research programs and external users. Through these applications neutron beam line diagnostics have been shown to make important contributions to a number of key research areas in South Africa. As a result these techniques are now being viewed as 'standard and essential' for an increasing number of researchers who came to appreciate the extra dimension of knowledge provided by neutron techniques. In addition neutron beam line facilities provide excellent training platforms for human capacity building in nuc lear and material related science and technology. Because of these reasons neutron beam line facilities at research reactors offer unique opportunities to build productive cross-cutting research collaborations, at national and regional levels. Some information on the role that nuclear beams can play, in the capacities mentioned, will be shared by virtue of some examples and the national, international and regional net-working potential of research reactor based neutron facilities shall be discussed.

  7. Influence of Laccase and Tyrosinase on the Antioxidant Capacity of Selected Phenolic Compounds on Human Cell Lines

    Directory of Open Access Journals (Sweden)

    Matthias Riebel

    2015-09-01

    Full Text Available Polyphenolic compounds affect the color, odor and taste of numerous food products of plant origin. In addition to the visual and gustatory properties, they serve as radical scavengers and have antioxidant effects. Polyphenols, especially resveratrol in red wine, have gained increasing scientific and public interest due to their presumptive beneficial impact on human health. Enzymatic oxidation of phenolic compounds takes place under the influence of polyphenol oxidases (PPO, including tyrosinase and laccase. Several studies have demonstrated the radical scavenger effect of plants, food products and individual polyphenols in vitro, but, apart from resveratrol, such impact has not been proved in physiological test systems. Furthermore, only a few data exist on the antioxidant capacities of the enzymatic oxidation products of phenolic compounds generated by PPO. We report here first results about the antioxidant effects of phenolic substances, before and after oxidation by fungal model tyrosinase and laccase. In general, the common chemical 2,2-diphenyl-1-picrylhydrazyl assay and the biological tests using two different types of cell cultures (monocytes and endothelial cells delivered similar results. The phenols tested showed significant differences with respect to their antioxidant activity in all test systems. Their antioxidant capacities after enzymatic conversion decreased or increased depending on the individual PPO used.

  8. Effects of Running Shoes with Abrasion Resistant Rubber Sole on the Exercise Capacity of the Human Body

    Directory of Open Access Journals (Sweden)

    Bo Wang

    2017-10-01

    Full Text Available With the development of industrialization, rubber has been gradually used in the manufacture of sports equipment for its favourable properties. This study involved the addition of C5 petroleum resin into brominated isobutylene-isoprene rubber (BIIR and butadiene rubber (BR while manufacturing the sole of running shoes. The effects of running shoes with abrasion resistant rubber sole on the exercise capacity of the human body were investigated by analysing the skid resistance and abrasion resistance of the running shoes, and conducting biomechanical study on naked feet and feet wearing the shoes. The results demonstrated that the rubber sole had favourable slip resistance property and mechanical properties such as stretching, abrasion resistance, and hardness. Compared to naked feet, the peak pressure intensity of the whole step of feet wearing the newly developed shoes, was significantly lower than that of feet wearing ordinary shoes. In the future, rubber can bring more comfortable experience because of its favourable properties.

  9. Transgenic cassava lines carrying heterologous alternative oxidase ...

    African Journals Online (AJOL)

    Afuape

    2013-07-03

    Jul 3, 2013 ... production of flowers, apomixis (Nassar et al., 2000; ... In order to increase the stress tolerance capacity of ... stress-related procedure due to the activities of auxin ... the evaluation of the transgenic lines for rate of OES .... Some transgenic lines carrying the 35S-AOX fragment amplified using 35S303F1 and.

  10. ANALYSIS ABSORPTION CAPACITY OF EUROPEAN FUNDS UNDER THE OPERATIONAL PROGRAMME HUMAN RESOURCES DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    DANIELA FLORESCU

    2013-10-01

    Full Text Available One of the main goals of the European Union is the economic progress. In the last 50 years, and especially beginning with the ‘80s, remarkable efforts have been made for removing the borders between the EU national economies and for creating a unique market where goods, persons, capital and services could move freely. Commercial interchanges between UE states have significantly grown and at the same time EU has become a global commercial force. EU’s goal is to become the most dynamic economy based on global recognition. This implies a significant investment in research, education and forming, which allows the population to have access to this new information. This research work displays diverse aspects concerning the Romania’s ability draw of irredeemable funds in period 2007 – 2013, focusing on human capital development activity. Today, the problem absorptions are no longer able to develop projects, that knowing a significant improvement but the stage of implementation and funding.

  11. Evaluation of anti-hepatocarcinoma capacity of puerarin nanosuspensions against human HepG2 cells

    Science.gov (United States)

    Meng, Xiang-Ping; Zhang, Zhen; Wang, Yi-Fei; Wang, Zhi-ping; Chen, Tong-sheng

    2017-02-01

    Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Puerarin (Pue), a major active ingredient in the traditional Chinese medicine Gegen, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Pue nanosuspension (Pue-NS) composed of Pue and poloxamer 188 was prepared by high pressure homogenization technique. The in vitro anti-hepatocarcinoma effects of Pue-NS relative to efficacy of bulk Pue were evaluated. The particle size and zeta potential of Pue-NS were 218.5 nm and -18.8 mV, respectively. MTT assay showed that Pue-NS effectively inhibited the proliferation of HepG2 cells, and the corresponding IC50 values of Pue-NS and bulk Pue were 3.39 and 5.73 μg/ml. These results suggest that the delivery of Pue-NS is a promising approach for treating tumors.

  12. 基于旅游生产线的景区容量计算模型构建及与传统模式的比较分析%The construction of tourism space carrying capacity calculation model based on production line and the competitive with traditional models

    Institute of Scientific and Technical Information of China (English)

    鄢慧丽

    2011-01-01

    Recently, in several articles, scholar Huang Yangshan analyzed the method of calculating the carry capacity of scenic area, and pointed out the limitation of traditional method based on the turnover. Then he deduced an improved model. But in his studies there is no comparison analysis to the difference of the optimal capacity value of the models in different conditions, and his improved model still has some limitation. Therefore, this paper first presents a new tourism scenic capacity calculation model based on production line, then analyses the balance of the attraction time and the attraction capacity of the scenic depot, which is the important fact influencing the volume of the carry capacity. And then, we compared our new model with the traditional ones in different condition. The results show that the new model based on tourism production line is suitable for wider scope, and is more reasonable.%近期,黄羊山在多篇文章中对风景区旅游空间容量的方法进行了探讨,且指出了传统周转率法的局限性,并说明了模型错误的原因.但其分析偏于定性,且没有指出不同条件下,这些模型求得的容量值的情况.本文首先提出了一种新的基于旅游生产线的景区容量计算模型,接着分析了景点时间和景点容量均衡与否对景区容量计算的影响,并对新的景区容量计算模型与传统景区容量计算模型进行了量化比较研究.结果表明基于旅游生产线的景区容量计算模型适用范围更广、更具有一般性.

  13. Biolingüística y capacidad humana Biolinguistics and Human Capacity

    Directory of Open Access Journals (Sweden)

    Chomsky Noam Abraham

    2006-06-01

    Full Text Available Este artículo presenta de manera sucinta el enfoque llamado 'biolingüístico' que empezó a desarrollarse en los primeros años de la posguerra, teniendo en cuenta los avances de la biología y las matemáticas. Se pasa revista a algunos postulados de la neurociencia (i.e. ciencia cognitiva en lo concerniente a la 'facultad del lenguaje', así como a los dos últimos modelos de la GG: el modelo de 'Principios y Parámetros' (P&P y el modelo 'Minimalista'. Estos últimos buscan responder las cuestiones fundamentales de la biología del lenguaje, su naturaleza, uso y evolución. Desde este punto de vista, se trata de caracterizar el equipamiento biológico gracias al cual los seres humanos adquieren una gramática. Así las cosas, se considera el equipamiento biológico como una función que asocia experiencia y gramática.This paper depicts a brief account of the so-called ´biolinguistic´ approach which dates back to the first postwar years, by taking into account progress in biology and mathematics. Some postulates of neuroscience (i.e. cognitive science regarding the ´faculty of language´ as well as the last two models of GG: the ´Principles and Parameters´ (P&P and the ´Minimalist´ models are reviewed. The latter aim to provide answers to key questions on the biology of language, its nature, its use, and its evolution. From this standpoint it has been attempted to characterize the biological endowment which allows human beings to acquire a grammar. Thus the biological endowment is seen as a function relating experience to grammar.

  14. The effect of gestational diabetes on proliferation capacity and viability of human umbilical cord-derived stromal cells.

    Science.gov (United States)

    Wajid, Nadia; Naseem, Rashida; Anwar, Sanam Saiqa; Awan, Sana Javaid; Ali, Muhammad; Javed, Sara; Ali, Fatima

    2015-09-01

    Stomal cells derived from Wharton's jelly of human umbilical cord (WJMSCs) are considered as the potential therapeutic agents for regeneration and are getting famous for stem cell banking. Our study aims to evaluate the effects of gestational diabetes on proliferation capacity and viability of WJMSCs. Mesenchymal stromal cells were isolated from Wharton's jelly of human umbilical cords from normal and gestational diabetic (DWJMSCs) mothers. Growth patterns of both types of cells were analyzed through MTT assay and population doubling time. Cell survival, cell death and glucose utilization were estimated through trypan blue exclusion assay, LDH assay and glucose detection assay respectively. Angiogenic ability was evaluated by immunocytochemistry and ELISA for VEGF A. Anti-cancerous potential was analyzed on HeLa cells. DWJMSCs exhibited low proliferative rate, increased population doubling time, reduced cell viability and increased cell death. Interestingly, DWJMSCs were found to have a reduced glucose utilization and anti-cancerous ability while enhanced angiogenic ability. Gestational diabetes induces adverse effects on growth, angiogenic and anti-cancerous potential of WJMSCs.

  15. Region-wide assessment of the capacity for human nutrition training in West Africa: current situation, challenges, and way forward

    Directory of Open Access Journals (Sweden)

    Roger Sodjinou

    2014-01-01

    Full Text Available Background: There is a dearth of information on existing nutrition training programs in West Africa. A preliminary step in the process of developing a comprehensive framework to strengthen human capacity for nutrition is to conduct an inventory of existing training programs. Objective: This study was conducted to provide baseline data on university-level nutrition training programs that exist in the 16 countries in West Africa. It also aimed to identify existing gaps in nutrition training and propose solutions to address them. Design: Participating institutions were identified based on information provided by in-country key informants, UNICEF offices or through internet searches. Data were collected through semi-structured interviews during on-site visits or through self-administered questionnaires. Simple descriptive and bivariate analyses were performed. Results: In total, 83 nutrition degree programs comprising 32 B.Sc. programs, 34 M.Sc. programs, and 17 Ph.D. programs were identified in the region. More than half of these programs were in Nigeria. Six countries (Cape Verde, Guinea-Bissau, Liberia, Mali, The Gambia, and Togo offered no nutrition degree program. The programs in francophone countries were generally established more recently than those in anglophone countries (age: 3.5 years vs. 21.4 years. Programs were predominantly (78% run by government-supported institutions. They did not provide a comprehensive coverage of all essential aspects of human nutrition. They were heavily oriented to food science (46%, with little emphasis on public health nutrition (24% or overnutrition (2%. Annual student intakes per program in 2013 ranged from 3 to 262; 7 to 40; and 3 to 10, respectively, for bachelor's, master's, and doctoral programs while the number of graduates produced annually per country ranged from 6 to 271; 3 to 64; and 1 to 18, respectively. External collaboration only existed in 15% of the programs. In-service training programs on

  16. Relationship between human resource ability and market access capacity on business performance. (case study of wood craft micro- and small-scale industries in Gianyar Regency, Bali)

    Science.gov (United States)

    Sukartini, N. W.; Sudarmini, N. M.; Lasmini, N. K.

    2018-01-01

    The aims of this research are to: (1) analyze the influence of Human Resource Ability on market access capacity in Wood Craft Micro and Small Industry; (2) to analyze the effect of market access capacity on business performance; (3) analyze the influence of Human Resources ability on business performance. Data were collected using questionnaires, interviews, observations, and literature studies. The resulting data were analyzed using Struture Equation Modeling (SEM). The results of the analysis show that (1) there is a positive and significant influence of the ability of Human Resources on market access capacity in Wood Craft Micro-and Small-Scale Industries in Gianyar; (2) there is a positive and significant influence of market access capacity on business performance; and (3) there is a positive and significant influence of Human Resource ability on business performance. To improve the ability to access the market and business performance, it is recommended that human resource ability need to be improved through training; government and higher education institutions are expected to play a role in improving the ability of human resources (craftsmen) through provision of training programs

  17. Nanoelectrospray high capacity ion trap multiple stage mass spectrometry for the structural analysis of human brain gangliosides

    International Nuclear Information System (INIS)

    Vukelic, Zeljka; Ratiu, Cornelia; Grozescu, Ioan; Zamfir, Alina Diana

    2006-01-01

    Full text: A novel protocol based on electrospray ionization (ESI) multiple stage high capacity ion trap (HCT) mass spectrometry (MS) was developed for glycosphingolipidomic surveys. The method was optimized for detailed structural elucidation of human brain gangliosides and particularly applied to human hippocampus-associated structures. The multiple stage MS experiments allowed for a complete structural characterization of GM1 ganglioside species, which was achieved by elucidation of the oligosaccharide sequence, identification of the GM1 a structural isomer from the data upon sialic acid localization along the sugar backbone and determination of the d18:1/18:0 of fatty acid/sphingoid base composition of the ceramide moiety. The methodology developed here is of general practical applicability for glycolipids and represents a step forward in the implementation of the advanced and most modern MS methods in glycomics. Gangliosides are glycosphingolipids, which consist of a mono- to polysialylated oligosaccharide chain of variable length attached to a ceramide portion of different composition with respect to the type of sphingoid base and fatty acid residues. Among all body systems, the central nervous system (CNS) possesses the highest content of gangliosides and they are playing a particularly important biological role at this level. Specific changes in the ganglioside expression and type of the expressed structures were observed to occur during brain development, maturation, and aging, and due to diseases or neurodegeneration processes. Gangliosides represent, therefore, an important class of biomarkers, carriers of information upon various CNS processes and events. Though in the human brain, their expression was observed to have a regional and tissue development induced specificity, the differences in ganglioside structure, composition and quantity were not systematically investigated or rigorously determined so far. (authors)

  18. TiO2 coating promotes human mesenchymal stem cell proliferation without the loss of their capacity for chondrogenic differentiation

    International Nuclear Information System (INIS)

    Kaitainen, Salla; Lappalainen, Reijo; Mähönen, Anssi J; J Lammi, Mikko; Qu, Chengjuan; Kröger, Heikki

    2013-01-01

    Human mesenchymal stem cells (hMSCs) are used in applications, which may require a large amount of cells; therefore, efficient expansion of the cells is desired. We studied whether TiO 2 coating on plastic cell culture dishes could promote proliferation of hMSCs without adverse effects in chondrogenic differentiation. TiO 2 -films were deposited on polystyrene dishes and glass coverslips using an ultrashort pulsed laser deposition technique. Human MSCs from three donors were expanded on them until 95% confluence, and the cells were evaluated by morphology, immunocytochemistry and quantitative RT-PCR (qRT-PCR). The chondrogenic differentiation in pellets was performed after cultivation on TiO 2 -coated dishes. Chondrogenesis was evaluated by histological staining of proteoglycans and type II collagen, and qRT-PCR. Human MSC-associated markers STRO-1, CD44, CD90 and CD146 did not change after expansion on TiO 2 -coated coverslips. However, the cell number after a 48h-culture period was significantly higher on TiO 2 -coated culture dishes. Importantly, TiO 2 coating caused no significant differences in the proteoglycan and type II collagen staining of the pellets, or the expression of chondrocyte-specific genes in the chondrogenesis assay. Thus, the proliferation of hMSCs could be significantly increased when cultured on TiO 2 -coated dishes without weakening their chondrogenic differentiation capacity. The transparency of TiO 2 -films allows easy monitoring of the cell growth and morphology under a phase-contrast microscope. (paper)

  19. The effect of pro-inflammatory cytokines on immunophenotype, differentiation capacity and immunomodulatory functions of human mesenchymal stem cells.

    Science.gov (United States)

    Pourgholaminejad, Arash; Aghdami, Nasser; Baharvand, Hossein; Moazzeni, Seyed Mohammad

    2016-09-01

    Mesenchymal stem cells (MSCs), as cells with potential clinical utilities, have demonstrated preferential incorporation into inflammation sites. Immunophenotype and immunomodulatory functions of MSCs could alter by inflamed-microenvironments due to the local pro-inflammatory cytokine milieu. A major cellular mediator with specific function in promoting inflammation and pathogenicity of autoimmunity are IL-17-producing T helper 17 (Th17) cells that polarize in inflamed sites in the presence of pro-inflammatory cytokines such as Interleukin-1β (IL-1β), IL-6 and IL-23. Since MSCs are promising candidate for cell-based therapeutic strategies in inflammatory and autoimmune diseases, Th17 cell polarizing factors may alter MSCs phenotype and function. In this study, human bone-marrow-derived MSCs (BM-MSC) and adipose tissue-derived MSCs (AD-MSC) were cultured with or without IL-1β, IL-6 and IL-23 as pro-inflammatory cytokines. The surface markers and their differentiation capacity were measured in cytokine-untreated and cytokine-treated MSCs. MSCs-mediated immunomodulation was analyzed by their regulatory effects on mixed lymphocyte reaction (MLR) and the level of IL-10, TGF-β, IL-4, IFN-γ and TNF-α production as immunomodulatory cytokines. Pro-inflammatory cytokines showed no effect on MSCs morphology, immunophenotype and co-stimulatory molecules except up-regulation of CD45. Adipogenic and osteogenic differentiation capacity increased in CD45+ MSCs. Moreover, cytokine-treated MSCs preserved the suppressive ability of allogeneic T cell proliferation and produced higher level of TGF-β and lower level of IL-4. We concluded pro-inflammatory cytokines up-regulate the efficacy of MSCs in cell-based therapy of degenerative, inflammatory and autoimmune disorders. Copyright © 2016. Published by Elsevier Ltd.

  20. Tourism environmental carrying capacity of Hebei province based on Theil coefficient and improved ecological footprint method%基于锡尔系数及改进生态足迹的河北省旅游环境承载力研究

    Institute of Scientific and Technical Information of China (English)

    褚英敏; 李素喜; 刘金平

    2014-01-01

    基于锡尔系数与改进后的生态足迹方法,分析了河北省旅游环境承载力。结果表明:从2001年到2012年,河北省旅游生态足迹与旅游生态承载力均呈现下降趋势,且总体表现出生态赤字状态,河北省旅游发展处于不可持续状态;空间上,在11个地级市中,秦皇岛的人均旅游生态足迹与人均旅游生态赤字均最高,沧州市最低;而张家口人均旅游生态承载力最高,唐山最低,从环渤海、环京津与环省会旅游圈来看,3个旅游圈之间的旅游环境承载力差异较小,但各旅游圈内各市的差异较大,其中以环渤海旅游圈内的差异最大;为了促进河北省旅游发展的可持续性,发展生态旅游、降低旅游生态赤字势在必行。%Based on Theil coefficient and the improved ecological footprint method,the tourism environmental carrying capacity of Hebei province is analyzed.The results showed that,the tourism ecological footprint per person(efT )and tourism ecological carrying capacity per person (ecT)in Hebei province showed a downward trend from 2001 to 2012,and the tourism develop-ment is in an unsustainable state because the tourism ecological deficit per person(ted)is positive in Hebei province.Spatially speaking,the efT and ted of Qinhuangdao are the highest in 11 cit-ies,and those of Cangzhou are lowest.While the tec of Zhangjiakou is highest and Tangshan is lowest.Between the 3 tourism circles,the difference of tourism environmental carrying capacity is small,but it is large in the circle.In order to promote the sustainable development of tourism, promotion of ecological tourism and reduction of tourism ecological deficit are imperative.

  1. Can Structural Adjustment and Carrying Capacity Promotion Promote the Tourism Economic Growth?-Case of the Three Coastal Economic Zones in Eastern China%结构调整、承载力提升能促进旅游经济增长吗?--以东部沿海三大经济区为例

    Institute of Scientific and Technical Information of China (English)

    刘佳; 周长晓

    2015-01-01

    With China’s Bohai coastal region,Yangtze river delta and Pan-pearl river delta coastal economic zone as the object of research,this paper evaluates and measures the three regions’tourism industry structure level and tourism ecological carrying index respectively from 2003 to 2012,and reveals the spatial difference of the coast-al three regions from the perspectives of structure and bearing capacity,then analyses and tests the effect of industri-al structure and bearing capacity on the tourism economic growth.The results show that three coastal regional tourism industrial structure transformed to rationality,the overall level of development is on the rise,but the Yangtze river delta and the Pearl river delta are instability,the fluctuation trend of tourism industry in Bohai Coastal region become smaller.The tourism ecology capacity of three regions has a slight increase,but tourism ecological demand is greater than the supply,it makes the three regions’tourism ecological in the deficit state,and the tourism ecological deficit is bigger and bigger,the promotion of tourism ecological carrying capacity and the tourism industry structure adjustment can effectively promote the tourism economic growth,but the positive effect of tourism industrial structure is greater than the tourist ecological carrying capacity.%以中国环渤海、长三角、泛珠三角三大沿海经济区为研究对象,分别对2003—2012年三大地区的旅游产业结构水平和旅游生态承载力指数进行测度和评价,并从结构和承载力视角,揭示沿海三大区域空间差异特征,进而分析和检验产业结构、承载力对旅游经济增长的作用。结果表明,2003—2012年中国沿海三大区域的旅游产业结构向合理化和高度化转化,总体发展水平呈上升趋势,但长三角、珠三角波动较大表现出不稳定性,环渤海地区旅游产业结构波动趋势逐渐变小。各地区旅游生态承载力略有提升

  2. TheApplication of Demarketing Theory to Adjusti ng Environmental Carrying Capacity of Tourism Resources---A Case Study of Ancient Water Towns in Southern Yangtze River%反营销理论在旅游资源群环境承载力调控中的应用--以江南水乡古镇旅游资源群为例

    Institute of Scientific and Technical Information of China (English)

    张晓帆; 谢芳

    2016-01-01

    With the case study of ancient water towns in southern Yangtze River , the uprising number of tourists makes a great pressure on the environmental carrying capacity of the tourism resources .It is suggested to use demarketing theory to adjust this .%文章从江南水乡古镇旅游资源群案例入手,分析不断攀升的客流对江南水乡旅游资源群的环境承载力所带来的影响,并提出运用反营销理论对江南水乡古镇旅游资源群环境承载力进行调控的对策及建议。

  3. Effects of marine cultural elements on coastal tourism:an empirical analysis of Zhoushan Archipelago-based cultural carrying capacity model%海洋文化要素在滨海旅游中的作用——以舟山群岛为例构建文化承载力模型实证分析

    Institute of Scientific and Technical Information of China (English)

    汤学敏; 胡麦秀

    2012-01-01

    在推进海洋文化建设的背景下对文化要素在滨海旅游中的作用进行量化,基于对滨海旅游文化承载力的分析,以舟山地区为例测量出该地区对文化感知度或是接受容纳程度的数值,结果表明:文化因素在滨海旅游中具有重要作用,并提出了加大滨海旅游中文化建设的建议.%In the background of promoting construction of marine culture the effects of cultural elements on coastal tourism were quantitatively analyzed,and the cultural perceptibility or acceptable capacity in Zhoushan area was calculated by analyzing the cultural carrying capacity of coastal tourism.lt was concluded that cultural elements had an important effect on coastal tourism. Also the suggestions and measures were put forward to enhance the cultural construction in coastal tourism.

  4. Towards an Informed Mexican and Mexican-American Citizenry: Bridging the Gap to Increase Human Capacity and Information Dissemination

    Science.gov (United States)

    Ramirez, M. D.; Ramirez, D. M.

    2008-12-01

    The research translation and community outreach goal of The University of Arizona's (UA) Superfund Basic Research Program and U.S.-Mexico Binational Center for Environmental Sciences and Toxicology is to increase human capacity and information dissemination to diverse stakeholders, including federal, state, and local government agencies as well as northern Mexican and border community stakeholders. Due to Arizona's demographic characteristics and the UA's proximity to the U.S. - Mexico border, activities target primarily Mexican and Mexican-American populations. With this in mind, a model has been established that pulls from human capital, community-based participatory research and public participation theories. The theories applied to our target population have resulted in the creation of a successful model that is used in both research translation and community outreach work. The model contains four components: community needs (participation), science translation (information), engagement (outreach), and training (education). Examples of how this model operates for various stakeholders involved in environmental science and health issues will be discussed. A case in point of how this model has been applied effectively is the partnership with promotoras (community health advocates) to do environmental science and health trainings to increase the knowledge base of specific populations disproportionately exposed to contaminants of concern. Additional case studies and methodologies used to develop innovative communicative tools (that takes into consideration cultural idiosyncrasies) for stakeholders at all levels in Arizona, the border, and Mexico will be highlighted, such as: 1) information sheets regarding local environmental issues for communities neighboring contaminated sites, 2) SciTransfer Bulletins targeting professional level stakeholders such as Project Managers, Community Involvement Coordinators and the general public, 3) coordinating technical and

  5. Loss of proliferation and differentiation capacity of aged human periodontal ligament stem cells and rejuvenation by exposure to the young extrinsic environment.

    Science.gov (United States)

    Zheng, Wei; Wang, Shi; Ma, Dandan; Tang, Liang; Duan, Yinzhong; Jin, Yan

    2009-09-01

    The application of periodontal ligament stem cells (PDLSCs) may be effective for periodontal regenerative therapy. As tissue regenerative potential may be negatively regulated by aging, whether aging and its microenvironment modify human PDLSCs remains a question. In this study, we compared the proliferation and differentiation capacity of PDLSCs obtained from young and aged donors. Then, we exposed aged PDLSCs to young periodontal ligament cell-conditioned medium (PLC-CM), and young PDLSCs were exposed to aged PLC-CM. Morphological appearance, colony-forming assay, cell cycle analysis, osteogenic and adipogenic induction media, gene expression of cementoblast phenotype, and in vivo differentiation capacities of PDLSCs were evaluated. PDLSCs obtained from aged donors exhibited decreased proliferation and differentiation capacity when compared with those from young donors. Young PLC-CM enhanced the proliferation and differentiation capacity of PDLSCs from aged donors. Aged PDLSCs induced by young PLC-CM showed enhanced tissue-regenerative capacity to produce cementum/periodontal ligament-like structures, whereas young PDLSCs induced by aged PLC-CM transplants mainly formed connective tissues. To our knowledge, this is the first study to mimic the developmental microenvironment of PDLSCs in vitro, and our data suggest that age influences the proliferation and differentiation potential of human PDLSCs, and that the activity of human PDLSCs can be modulated by the extrinsic microenvironment.

  6. A Sensitive and Rapid Method to Determine the Adhesion Capacity of Probiotics and Pathogenic Microorganisms to Human Gastrointestinal Mucins

    Directory of Open Access Journals (Sweden)

    Bélinda Ringot-Destrez

    2018-05-01

    Full Text Available Mucus is the habitat for the microorganisms, bacteria and yeast that form the commensal flora. Mucins, the main macromolecules of mucus, and more specifically, the glycans that cover them, play essential roles in microbial gastrointestinal colonization. Probiotics and pathogens must also colonize mucus to have lasting positive or deleterious effects. The question of which mucin-harboured glycan motifs favour the adhesion of specific microorganisms remains very poorly studied. In the current study, a simple test based on the detection of fluorescent-labeled microorganisms raised against microgram amounts of mucins spotted on nitrocellulose was developed. The adhesion of various probiotic, commensal and pathogenic microorganisms was evaluated on a panel of human purified gastrointestinal mucins and compared with that of commercially available pig gastric mucins (PGM and of mucins secreted by the colonic cancer cell line HT29-MTX. The latter two proved to be very poor indicators of adhesion capacity on intestinal mucins. Our results show that the nature of the sialylated cores of O-glycans, determined by MALDI MS-MS analysis, potentially enables sialic acid residues to modulate the adhesion of microorganisms either positively or negatively. Other identified factors affecting the adhesion propensity were O-glycan core types and the presence of blood group motifs. This test should help to select probiotics with enhanced adhesion capabilities as well as deciphering the role of specific mucin glycotopes on microbial adhesion.

  7. Examination of the roles and capacities of duty bearers responsible for protecting the human rights to adequate food, nutritional health and wellbeing in Ugandan children's homes.

    Science.gov (United States)

    Olafsen, Monica; Rukooko, Archangel Byaruhanga; Iversen, Per Ole; Andreassen, Bård A

    2018-04-17

    The majority of Ugandan children face vulnerability and malnutrition. As a State Party to international human rights treaties, Uganda has legal obligations of guaranteeing the fundamental rights and the best interest of the nation's children. Despite being protected under international and national law, Uganda is not providing adequate child protection, including safeguarding children's food security. Numerous privately owned and unregulated children's homes face this problem. The overall aim of the study was to examine to what extent children's homes' operations are consistent with the right to adequate food, nutritional health and wellbeing of children. We performed a qualitative role- and capacity analysis of duty bearers with human rights duties towards children living in children's homes. We studied three groups of duty bearers: caretakers working in private children's homes, State actors working in government and its institutions, and non-State actors working in civil society organizations. A human rights based approach guided all aspects of the study. An analysis of the roles, performance and capacities of duty bearers was employed, with individual face-to-face structured qualitative in-depth interviews, self-administered structured questionnaires, and a structured observational study, as well as a desk review of relevant literature. The State of Uganda's efforts to respect and realize its obligations towards children living in children's homes is inadequate. There are numerous capacity gaps among the duty bearers, and the concepts of human rights and the best interest of the child are not well understood among the duty bearers. The efforts of the State of Uganda to realize its human rights obligations towards children in children's homes are lacking in important areas. Hence the State does not fulfill its minimum obligations under the International Covenant on Economic, Social and Cultural Rights to ensure all children freedom from hunger. There is a need

  8. Human Resources Capacity Building as a Strategy in Strengthening Nuclear Knowledge Sustainability in the Experimental Fuel Element Installation of BATAN-Indonesia

    International Nuclear Information System (INIS)

    Ratih Langenati; Bambang, Herutomo; Arief Sasongko Adhi

    2014-01-01

    Strategy in Strengthening Nuclear Knowledge Sustainability: • In order to maintain human resources capacity related to nuclear fuel production technology, a nuclear knowledge preservation program is implemented in the EFEI. • The program includes coaching/training, mentoring and documenting important knowledge. • The program activities are monitored and evaluated quarterly for its improvement in the following year

  9. The experimental investigation of glioma-trophic capacity of human umbilical cord-derived mesenchymal stem cells after intraventricular administration

    Directory of Open Access Journals (Sweden)

    FAN Cun-gang

    2013-07-01

    Full Text Available Objective To explore the glioma-trophic migration capacity of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs by intraventricular administration. Methods The umbilical cord tissue were obtained during full-term pregnancy cesarean section under sterile conditions. This study was approved by Ethics Committee and got the informed consent of patient. The hUC-MSCs were isolated by trypsin and collagenase digestion, followed by adherent culture methods. The characteristics of isolated hUC-MSCs were demonstrated by cell morphylogy, phenotype analysis and multi-differentiation potentials into adipocytes, osteoblasts and neural cells. Then the hUC-MSCs were labeled with CM-DiI and injected into contralateral ventricle of glioma of the C6 glioma-bearing Sprague-Dawley (SD rats. Two weeks later, the rats were sacrificed and the brains were taken out to examine the migration and distribution of hUC-MSCs in the tumor bed, at the interface of tumor and cerebral parenchyma as well as the tumor satelites infiltrating into the normal brain. Results The hUC-MSCs demonstrated plastic-adherent characterization and homogeneous fibroblastic-like morphylogy in culture, expression of specific surface phenotypes of MSCs (CD13, CD29, CD44, CD90 but not endothelial or hematopoietic markers (CD14, CD31, CD34, CD38, CD45, CD133, and muti-differentiatiation potentials into Oil red O stained adipocytes, Alizarin red S stained osteoblasts, neuron-specific enolase (NSE-positive neurons and glial fibrillary acidic protein (GFAP-positive astrocytes in permissive inducive conditions. Importantly, after labeled hUC-MSCs injection into contralateral ventricle of glioma, the hUC-MSCs migrated from initial injection site to the glioma mass and along the interface of tumor and brain, and some of them "chasing" the glioma satellites infiltrated into the normal parenchyma. Conclusion The hUC-MSCs possess prominent tumor-specific targeting capacity and extensive intratumoral

  10. In normal human fibroblasts variation in DSB repair capacity cannot be ascribed to radiation-induced changes in the localisation, expression or activity of major NHEJ proteins

    DEFF Research Database (Denmark)

    Kasten-Pisula, Ulla; Vronskaja, Svetlana; Overgaard, Jens

    2008-01-01

    in the activity of the DNA-PK complex induced upon irradiation. CONCLUSIONS: For normal human fibroblasts, the level or activity of NHEJ proteins measured prior to or after irradiation cannot be used to predict the DSB repair capacity or cellular radiosensitivity. Udgivelsesdato: 2008-Mar......BACKGROUND AND PURPOSE: The aim of the present study was to test whether for normal human fibroblasts the variation in double-strand break (DSB) repair capacity results from radiation-induced differences in localisation, expression or activity of major non-homologous end-joining (NHEJ) proteins....... MATERIALS AND METHODS: Experiments were performed with 11 normal human fibroblast strains AF01-11. NHEJ proteins were determined by Western blot and DNA-PK activity by pulldown-assay. RESULTS: The four NHEJ proteins tested (Ku70, Ku80, XRCC4 and DNA-PKcs) were found to be localised almost exclusively...

  11. A research agenda for helminth diseases of humans: health research and capacity building in disease-endemic countries for helminthiases control.

    Directory of Open Access Journals (Sweden)

    Mike Y Osei-Atweneboana

    Full Text Available Capacity building in health research generally, and helminthiasis research particularly, is pivotal to the implementation of the research and development agenda for the control and elimination of human helminthiases that has been proposed thematically in the preceding reviews of this collection. Since helminth infections affect human populations particularly in marginalised and low-income regions of the world, they belong to the group of poverty-related infectious diseases, and their alleviation through research, policy, and practice is a sine qua non condition for the achievement of the United Nations Millennium Development Goals. Current efforts supporting research capacity building specifically for the control of helminthiases have been devised and funded, almost in their entirety, by international donor agencies, major funding bodies, and academic institutions from the developed world, contributing to the creation of (not always equitable North-South "partnerships". There is an urgent need to shift this paradigm in disease-endemic countries (DECs by refocusing political will, and harnessing unshakeable commitment by the countries' governments, towards health research and capacity building policies to ensure long-term investment in combating and sustaining the control and eventual elimination of infectious diseases of poverty. The Disease Reference Group on Helminth Infections (DRG4, established in 2009 by the Special Programme for Research and Training in Tropical Diseases (TDR, was given the mandate to review helminthiases research and identify research priorities and gaps. This paper discusses the challenges confronting capacity building for parasitic disease research in DECs, describes current capacity building strategies with particular reference to neglected tropical diseases and human helminthiases, and outlines recommendations to redress the balance of alliances and partnerships for health research between the developed countries of

  12. 北京市旅游人口与旅游承载容量适配度分析及策略研究%The adaptation degree analysis and strategy research of the tourist population and tourism carrying capacity in Beijing

    Institute of Scientific and Technical Information of China (English)

    王国力; 付硕天; 仲声

    2015-01-01

    北京市是我国的旅游业发展的核心地带之一,旅游产业发展规模大、程度高、速度快,但伴随着高速发展的旅游产业,旅游景区负荷过大,旅游人口与旅游承载容量不协调的问题已经日益凸显,以旅游承载容量为研究方法,以旅游人口为研究视角,对北京市的旅游产业的发展和负荷状况进行评价,并从管理运营制度,宣传,旅游产品形式三个方面提出优化改造和完善的对策,作为北京市解决旅游景区负荷和旅游人口过度密集等问题的一些政策参考,以期实现北京市旅游产业在可持续开发的前提下创造最大利润。%Beijing is one of the core tourism areas in our country whose tourism industry has large scale development,high degree and high speed.But with the rapid development of tourism industry,the load of tourism scenic area is too large and the incompatible problem of population and tourism carrying capacity has been growing. This paper takes tourism carrying capacity as research methods,tourist population as research perspectives,and evaluates the development and load conditions of Beijing's tourism industry,thus puts forward some countermeasures for optimizing reconstruction and perfection from the management operation system,publicity and tourism product. As policy references which aim to solve such problems as overcrowding and the load of tourism scenic spots,we hope Beijing tourism industry can create the largest profit on the premise of sustainable development.

  13. BLOOD SUBSTITUTES: EVOLUTION FROM NON-CARRYING TO OXYGEN AND GAS CARRYING FLUIDS

    Science.gov (United States)

    Cabrales, Pedro; Intaglietta, Marcos

    2013-01-01

    The development of oxygen (O2) carrying blood substitutes has evolved from the goal of replicating blood O2 transports properties to that of preserving microvascular and organ function, reducing the inherent or potential toxicity of the material used to carry O2, and treating pathologies initiated by anemia and hypoxia. Furthermore, the emphasis has shifted from blood replacement fluid to “O2 therapeutics” that restore tissue oxygenation to specific tissues regions. This review covers the different alternatives, potential and limitations of hemoglobin based O2 carriers (HBOCs) and perfluorocarbon based O2 carriers (PFCOCs), with emphasis on the physiological conditions disturbed in the situation that they will be used. It describes how concepts learned from plasma expanders without O2 carrying capacity can be applied to maintain O2 delivery and summarizes the microvascular responses due to HBOCs and PFCOCs. This review also presents alternative applications of HBOCs and PFCOCs namely: 1) How HBOC O2 affinity can be engineered to target O2 delivery to hypoxic tissues; and 2) How the high gas solubility of PFCOCs provides new opportunities for carrying, dissolving and delivering gases with biological activity. It is concluded that current blood substitutes development has amplified their applications horizon by devising therapeutic functions for oxygen carriers requiring limited O2 delivery capacity restoration. Conversely, full, blood-like O2 carrying capacity re-establishment awaits control of O2 carrier toxicity. PMID:23820271

  14. Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene.

    Science.gov (United States)

    Meraviglia, Viviana; Benzoni, Patrizia; Landi, Sara; Murano, Carmen; Langione, Marianna; Motta, Benedetta M; Baratto, Serena; Silipigni, Rosamaria; Di Segni, Marina; Pramstaller, Peter P; DiFrancesco, Dario; Gazzerro, Elisabetta; Barbuti, Andrea; Rossini, Alessandra

    2018-03-01

    Caveolinopathies are a heterogeneous family of genetic pathologies arising from alterations of the caveolin-3 gene (CAV3), encoding for the isoform specifically constituting muscle caveolae. Here, by reprogramming peripheral blood mononuclear cells, we report the generation of induced pluripotent stem cells (iPSCs) from three patients carrying the ΔYTT deletion, T78K and W101C missense mutations in caveolin-3. iPSCs displayed normal karyotypes and all the features of pluripotent stem cells in terms of morphology, specific marker expression and ability to differentiate in vitro into the three germ layers. These lines thus represent a human cellular model to study the molecular basis of caveolinopathies. Resource table. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  15. Capacity-speed relationships in prefrontal cortex.

    Directory of Open Access Journals (Sweden)

    Vivek Prabhakaran

    Full Text Available Working memory (WM capacity and WM processing speed are simple cognitive measures that underlie human performance in complex processes such as reasoning and language comprehension. These cognitive measures have shown to be interrelated in behavioral studies, yet the neural mechanism behind this interdependence has not been elucidated. We have carried out two functional MRI studies to separately identify brain regions involved in capacity and speed. Experiment 1, using a block-design WM verbal task, identified increased WM capacity with increased activity in right prefrontal regions, and Experiment 2, using a single-trial WM verbal task, identified increased WM processing speed with increased activity in similar regions. Our results suggest that right prefrontal areas may be a common region interlinking these two cognitive measures. Moreover, an overlap analysis with regions associated with binding or chunking suggest that this strategic memory consolidation process may be the mechanism interlinking WM capacity and WM speed.

  16. Estimation of folate binding capacity (unsaturated and total) in normal human serum and in β-thalassaemia

    International Nuclear Information System (INIS)

    Moulopoulos, S.; Mantzos, J.; Gyftaki, E.; Kesse-Elias, M.; Alevizou-Terzaki, V.; Souli-Tsimili, E.

    1978-01-01

    A method is described for measuring the total serum folate binding capacity (TBC) after treating the serum with urea at pH5.5, the unsaturated serum folate binding capacity (UBC) being determined without treatment with urea. The method was applied to 50 normal controls and 20 patients with homozygous β-thalassaemia. The results show an increase in folate binding capacity after treating the serum with urea in all cases studied. There is no correlation between serum folic acid level and total or unsaturated folate binding capacity or per cent saturation. The method described is a simple and rapid one for screening the different groups studied for saturated and unsaturated specific folate-binding proteins. (author)

  17. Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity

    Directory of Open Access Journals (Sweden)

    Fu Freddie H

    2011-06-01

    Full Text Available Abstract Background The human anterior cruciate ligament (hACL and medial collateral ligament (hMCL of the knee joint are frequently injured, especially in athletic settings. It has been known that, while injuries to the MCL typically heal with conservative treatment, ACL injuries usually do not heal. As adult stem cells repair injured tissues through proliferation and differentiation, we hypothesized that the hACL and hMCL contain stem cells exhibiting unique properties that could be responsible for the differential healing capacity of the two ligaments. Methods To test the above hypothesis, we derived ligament stem cells from normal hACL and hMCL samples from the same adult donors using tissue culture techniques and characterized their properties using immunocytochemistry, RT-PCR, and flow cytometry. Results We found that both hACL stem cells (hACL-SCs and hMCL stem cells (hMCL-SCs formed colonies in culture and expressed stem cell markers nucleostemin and stage-specific embryonic antigen-4 (SSEA-4. Moreover, both hACL-SCs and hMCL-SCs expressed CD surface markers for mesenchymal stem cells, including CD44 and CD90, but not those markers for vascular cells, CD31, CD34, CD45, and CD146. However, hACL-SCs differed from hMCL-SCs in that the size and number of hACL-SC colonies in culture were much smaller and grew more slowly than hMCL-SC colonies. Moreover, fewer hACL-SCs in cell colonies expressed stem cell markers STRO-1 and octamer-binding transcription factor-4 (Oct-4 than hMCL-SCs. Finally, hACL-SCs had less multi-differentiation potential than hMCL-SCs, evidenced by differing extents of adipogenesis, chondrogenesis, and osteogenesis in the respective induction media. Conclusions This study shows for the first time that hACL-SCs are intrinsically different from hMCL-SCs. We suggest that the differences in their properties contribute to the known disparity in healing capabilities between the two ligaments.

  18. Intrapopulational body size variation and cranial capacity variation in Middle Pleistocene humans: the Sima de los Huesos sample (Sierra de Atapuerca, Spain).

    Science.gov (United States)

    Lorenzo, C; Carretero, J M; Arsuaga, J L; Gracia, A; Martínez, I

    1998-05-01

    A sexual dimorphism more marked than in living humans has been claimed for European Middle Pleistocene humans, Neandertals and prehistoric modern humans. In this paper, body size and cranial capacity variation are studied in the Sima de los Huesos Middle Pleistocene sample. This is the largest sample of non-modern humans found to date from one single site, and with all skeletal elements represented. Since the techniques available to estimate the degree of sexual dimorphism in small palaeontological samples are all unsatisfactory, we have used the bootstraping method to asses the magnitude of the variation in the Sima de los Huesos sample compared to modern human intrapopulational variation. We analyze size variation without attempting to sex the specimens a priori. Anatomical regions investigated are scapular glenoid fossa; acetabulum; humeral proximal and distal epiphyses; ulnar proximal epiphysis; radial neck; proximal femur; humeral, femoral, ulnar and tibial shaft; lumbosacral joint; patella; calcaneum; and talar trochlea. In the Sima de los Huesos sample only the humeral midshaft perimeter shows an unusual high variation (only when it is expressed by the maximum ratio, not by the coefficient of variation). In spite of that the cranial capacity range at Sima de los Huesos almost spans the rest of the European and African Middle Pleistocene range. The maximum ratio is in the central part of the distribution of modern human samples. Thus, the hypothesis of a greater sexual dimorphism in Middle Pleistocene populations than in modern populations is not supported by either cranial or postcranial evidence from Sima de los Huesos.

  19. AN INVESTIGATION OF RELATIONSHIP BETWEEN LEADERSHIP STYLES OF HUMAN RESOURCES MANAGER, CREATIVE PROBLEM SOLVING CAPACITY AND CAREER SATISFACTION: AN EMPIRICAL STUDY

    Directory of Open Access Journals (Sweden)

    Hüseyin YILMAZ

    2016-12-01

    Full Text Available The aim of this study is the creative problem-solving capacity of the organization with leadership behaviors of human resources managers and employees to examine the relationship between career satisfaction and is tested empirically. Research within the scope of the required data structured questionnaire method, operating in the province of Aydin was obtained from 130 employees working in five star hotels. Democratic leadership style according to the factor analysis, easygoing, participants converter, and releasing autocratic leadership dimensions were determined. According to the analysis, the dependent variable with a significant level of research and positive leadership style has been determined that no relationships. Regression analysis revealed that the leadership of the relationship with the creative problem-solving capacity of democratic leadership in style when found to be stronger than other leadership styles, while the variable describing the career of the employee satisfaction level of the maximum it was concluded that the creative problem-solving capacity of the organization. Research in the context of human resources on the very important for organizations, leadership behavior, creative problem-solving capacity and career satisfaction studies analyzing the relationships between variables it seems to be quite limited. The discovery by analyzing the relationship between the aforementioned variables, can make significant contributions to knowledge in the literature and are expected to form the basis for future research.

  20. Is the Oxidative DNA Damage Level of Human Lymphocyte Correlated with the Antioxidant Capacity of Serum or the Base Excision Repair Activity of Lymphocyte?

    Directory of Open Access Journals (Sweden)

    Yi-Chih Tsai

    2013-01-01

    Full Text Available A random screening of human blood samples from 24 individuals of nonsmoker was conducted to examine the correlation between the oxidative DNA damage level of lymphocytes and the antioxidant capacity of serum or the base excision repair (BER activity of lymphocytes. The oxidative DNA damage level was measured with comet assay containing Fpg/Endo III cleavage, and the BER activity was estimated with a modified comet assay including nuclear extract of lymphocytes for enzymatic cleavage. Antioxidant capacity was determined with trolox equivalent antioxidant capacity assay. We found that though the endogenous DNA oxidation levels varied among the individuals, each individual level appeared to be steady for at least 1 month. Our results indicate that the oxidative DNA damage level is insignificantly or weakly correlated with antioxidant capacity or BER activity, respectively. However, lymphocytes from carriers of Helicobacter pylori (HP or Hepatitis B virus (HBV tend to give higher levels of oxidative DNA damage (P<0.05. Though sera of this group of individuals show no particular tendency with reduced antioxidant capacity, the respective BER activities of lymphocytes are lower in average (P<0.05. Thus, reduction of repair activity may be associated with the genotoxic effect of HP or HBV infection.

  1. Cytotoxic Capacity of IL-15-Stimulated Cytokine-Induced Killer Cells Against Human Acute Myeloid Leukemia and Rhabdomyosarcoma in Humanized Preclinical Mouse Models

    Energy Technology Data Exchange (ETDEWEB)

    Rettinger, Eva; Meyer, Vida; Kreyenberg, Hermann [Department of Pediatric Hematology, Oncology and Hemostaseology, University Children’s Hospital of Frankfurt/Main, Goethe-University Frankfurt/Main, Frankfurt/Main (Germany); Volk, Andreas [Chemotherapeutisches Forschungsinstitut, Georg-Speyer-Haus, Frankfurt/Main (Germany); Kuçi, Selim; Willasch, Andre [Department of Pediatric Hematology, Oncology and Hemostaseology, University Children’s Hospital of Frankfurt/Main, Goethe-University Frankfurt/Main, Frankfurt/Main (Germany); Koscielniak, Ewa [Department of Pediatric Oncology and Hematology, Olgahospital Stuttgart, Stuttgart (Germany); Fulda, Simone [Institute for Experimental Cancer Research in Pediatrics, Goethe-University Frankfurt/Main, Frankfurt/Main (Germany); Wels, Winfried S. [Chemotherapeutisches Forschungsinstitut, Georg-Speyer-Haus, Frankfurt/Main (Germany); Boenig, Halvard [Institute for Transfusion Medicine and Immunohematology, Goethe-University Frankfurt/Main, Division for Cell Processing, German Red Cross Blood Donor Service Baden-Württemberg-Hessen, Frankfurt/Main (Germany); Klingebiel, Thomas; Bader, Peter, E-mail: eva.rettinger@kgu.de, E-mail: peter.bader@kgu.de [Department of Pediatric Hematology, Oncology and Hemostaseology, University Children’s Hospital of Frankfurt/Main, Goethe-University Frankfurt/Main, Frankfurt/Main (Germany)

    2012-04-09

    Allogeneic stem cell transplantation (allo-SCT) has become an important treatment modality for patients with high-risk acute myeloid leukemia (AML) and is also under investigation for soft tissue sarcomas. The therapeutic success is still limited by minimal residual disease (MRD) status ultimately leading to patients’ relapse. Adoptive donor lymphocyte infusions based on MRD status using IL-15-expanded cytokine-induced killer (CIK) cells may prevent relapse without causing graft-versus-host-disease (GvHD). To generate preclinical data we developed mouse models to study anti-leukemic- and anti-tumor-potential of CIK cells in vivo. Immunodeficient mice (NOD/SCID/IL-2Rγc{sup −}, NSG) were injected intravenously with human leukemic cell lines THP-1, SH-2 and with human rhabdomyosarcoma (RMS) cell lines RH41 and RH30 at minimal doses required for leukemia or tumor engraftment. Mice transplanted with THP-1 or RH41 cells were randomly assigned for analysis of CIK cell treatment. Organs of mice were analyzed by flow cytometry as well as quantitative polymerase chain reaction for engraftment of malignant cells and CIK cells. Potential of CIK cells to induce GvHD was determined by histological analysis. Tissues of the highest degree of THP-1 cell expansion included bone marrow followed by liver, lung, spleen, peripheral blood (PB), and brain. RH30 and RH41 engraftment mainly took place in liver and lung, but was also detectable in spleen and PB. In spite of delayed CIK cell expansion compared with malignant cells, CIK cells injected at equal amounts were sufficient for significant reduction of RH41 cells, whereas against fast-expanding THP-1 cells 250 times more CIK than THP-1 cells were needed to achieve comparable results. Our preclinical in vivo mouse models showed a reliable 100% engraftment of malignant cells which is essential for analysis of anti-cancer therapy. Furthermore our data demonstrated that IL-15-activated CIK cells have potent cytotoxic capacity

  2. AN INVESTIGATION OF RELATIONSHIP BETWEEN LEADERSHIP STYLES OF HUMAN RESOURCES MANAGER, CREATIVE PROBLEM SOLVING CAPACITY AND CAREER SATISFACTION: AN EMPIRICAL STUDY

    OpenAIRE

    Hüseyin YILMAZ

    2016-01-01

    The aim of this study is the creative problem-solving capacity of the organization with leadership behaviors of human resources managers and employees to examine the relationship between career satisfaction and is tested empirically. Research within the scope of the required data structured questionnaire method, operating in the province of Aydin was obtained from 130 employees working in five star hotels. Democratic leadership style according to the factor analysis, easygoing, participants c...

  3. Thymidine kinase deficient human cells have increased UV sensitivity in their capacity to support herpes simplex virus but normal UV sensitivity for colony formation

    International Nuclear Information System (INIS)

    Rainbow, A.J.

    1989-01-01

    A thymidine kinase deficient (tk - ) and two thymidine kinase proficient (tk + ) human cell lines were compared for UV sensitivity using colony-forming ability as well as their capacity to support the plaque formation of herpes simplex type 1 (HSV-1).The tk - line (143 cells) was a derivative of one of the tk + lines (R970-5), whereas the other tk + line (AC4 cells) was a derivative of the 143 cells obtained by transfection with purified sheared HSV-2 DNA encoding the viral tk gene. 143, R970-5 and AC4 cells showed a similar UV sensitivity for colony-forming ability. In contrast, the capacity to support HSV-1 plaque formation immediately (within 1 h) afte UV-irradiation was reduced to a greater extent in the 143 cells compared to the R970-5 and AC4 cells. Capacity curves for plaque formation of the HSV-1: KOS wild-type (tk + ) strain were similar to those for the HSV-1: PTK3B mutant (tk - ) strain were similar to those for the HSV-1: PTK3B mutant (tk - ) strain in the 3 cell strains, indicating that the viral tk gene does not influence the ability of HSV-1 to form plaques in UV-irradiated compared to unirradiated human cells. Cellular capacity for HSV-1 plaque formation was found to recover in both tk - and tk + cells for cultures infected 24 h after UV-irradiation. These results suggest that repair of UV-damaged DNA takes place to a similar extent in both tk - and tk + human cells, but the kinetics of repair are initially slower in tk - compared to tk + human cells. (author). 33 refs.; 3 figs.; 1 tab

  4. Managing Demand and Capacity Using Multi-Sector Planning and Flexible Airspace: Human-in-the-Loop Evaluation of NextGen

    Science.gov (United States)

    Lee, Paul U.; Smith, Nancy M.; Prevot, Thomas; Homola, Jeffrey R.

    2010-01-01

    When demand for an airspace sector exceeds capacity, the balance can be re-established by reducing the demand, increasing the capacity, or both. The Multi-Sector Planner (MSP) concept has been proposed to better manage traffic demand by modifying trajectories across multiple sectors. A complementary approach to MSP, called Flexible Airspace Management (FAM), reconfigures the airspace such that capacity can be reallocated dynamically to balance the traffic demand across multiple sectors, resulting in fewer traffic management initiatives. The two concepts have been evaluated with a series of human-in-the-loop simulations at the Airspace Operations Laboratory to examine and refine the roles of the human operators in these concepts, as well as their tools and procedural requirements. So far MSP and FAM functions have been evaluated individually but the integration of the two functions is desirable since there are significant overlaps in their goals, geographic/temporal scope of the problem space, and the implementation timeframe. Ongoing research is planned to refine the humans roles in the integrated concept.

  5. Expression of the human isoform of glutamate dehydrogenase, hGDH2, augments TCA cycle capacity and oxidative metabolism of glutamate during glucose deprivation in astrocytes

    DEFF Research Database (Denmark)

    Nissen, Jakob D; Lykke, Kasper; Bryk, Jaroslaw

    2017-01-01

    A key enzyme in brain glutamate homeostasis is glutamate dehydrogenase (GDH) which links carbohydrate and amino acid metabolism mediating glutamate degradation to CO2 and expanding tricarboxylic acid (TCA) cycle capacity with intermediates, i.e. anaplerosis. Humans express two GDH isoforms, GDH1...... and 2, whereas most other mammals express only GDH1. hGDH1 is widely expressed in human brain while hGDH2 is confined to astrocytes. The two isoforms display different enzymatic properties and the nature of these supports that hGDH2 expression in astrocytes potentially increases glutamate oxidation...

  6. Low Buffer Capacity and Alternating Motility along the Human Gastrointestinal Tract: Implications for in Vivo Dissolution and Absorption of Ionizable Drugs.

    Science.gov (United States)

    Hens, Bart; Tsume, Yasuhiro; Bermejo, Marival; Paixao, Paulo; Koenigsknecht, Mark J; Baker, Jason R; Hasler, William L; Lionberger, Robert; Fan, Jianghong; Dickens, Joseph; Shedden, Kerby; Wen, Bo; Wysocki, Jeffrey; Loebenberg, Raimar; Lee, Allen; Frances, Ann; Amidon, Greg; Yu, Alex; Benninghoff, Gail; Salehi, Niloufar; Talattof, Arjang; Sun, Duxin; Amidon, Gordon L

    2017-12-04

    In this study, we determined the pH and buffer capacity of human gastrointestinal (GI) fluids (aspirated from the stomach, duodenum, proximal jejunum, and mid/distal jejunum) as a function of time, from 37 healthy subjects after oral administration of an 800 mg immediate-release tablet of ibuprofen (reference listed drug; RLD) under typical prescribed bioequivalence (BE) study protocol conditions in both fasted and fed states (simulated by ingestion of a liquid meal). Simultaneously, motility was continuously monitored using water-perfused manometry. The time to appearance of phase III contractions (i.e., housekeeper wave) was monitored following administration of the ibuprofen tablet. Our results clearly demonstrated the dynamic change in pH as a function of time and, most significantly, the extremely low buffer capacity along the GI tract. The buffer capacity on average was 2.26 μmol/mL/ΔpH in fasted state (range: 0.26 and 6.32 μmol/mL/ΔpH) and 2.66 μmol/mL/ΔpH in fed state (range: 0.78 and 5.98 μmol/mL/ΔpH) throughout the entire upper GI tract (stomach, duodenum, and proximal and mid/distal jejunum). The implication of this very low buffer capacity of the human GI tract is profound for the oral delivery of both acidic and basic active pharmaceutical ingredients (APIs). An in vivo predictive dissolution method would require not only a bicarbonate buffer but also, more significantly, a low buffer capacity of dissolution media to reflect in vivo dissolution conditions.

  7. Glucagon-like peptide-1 (GLP-1) raises blood-brain glucose transfer capacity and hexokinase activity in human brain

    DEFF Research Database (Denmark)

    Gejl, Michael; Lerche, Susanne; Egefjord, Lærke

    2013-01-01

    phosphorylation velocity (V max) in the gray matter regions of cerebral cortex, thalamus, and cerebellum, as well as increased blood-brain glucose transport capacity (T max) in gray matter, white matter, cortex, thalamus, and cerebellum. In hypoglycemia, GLP-1 had no effects on net glucose metabolism, brain...

  8. Aged human bone marrow stromal cells maintaining bone forming capacity in vivo evaluated using an improved method of visualization

    DEFF Research Database (Denmark)

    Stenderup, Karin; Rosada, Cecilia; Justesen, J

    2004-01-01

    Age-related decreased osteoblast function is a well-known but poorly understood phenomenon. Previous studies that examined the effects of donor age on osteoblast functions employed in vitro assays that may not reflect the true osteoblast capacity for bone formation. Thus, we have developed an in ...

  9. The effect of moderate alcohol consumption on adiponectin oligomers and muscle oxidative capacity: A human intervention study

    NARCIS (Netherlands)

    Beulens, J.W.J.; Loon, L.J.C. van; Kok, F.J.; Pelsers, M.; Bobbert, T.; Spranger, J.; Helander, A.; Hendriks, H.F.J.

    2007-01-01

    Aims/hypothesis: The aim of this study was to investigate whether moderate alcohol consumption increases plasma high molecular weight (HMW) adiponectin and/or muscle oxidative capacity. Materials and methods: Eleven lean (BMI 18-25 kg/m2) and eight overweight (BMI ≥27 kg/m2) men consumed 100 ml

  10. DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis.

    Science.gov (United States)

    Bargues, María Dolores; Gayo, Valeria; Sanchis, Jaime; Artigas, Patricio; Khoubbane, Messaoud; Birriel, Soledad; Mas-Coma, Santiago

    2017-02-01

    Fascioliasis is a pathogenic disease transmitted by lymnaeid snails and recently emerging in humans, in part due to effects of climate changes, anthropogenic environment modifications, import/export and movements of livestock. South America is the continent presenting more human fascioliasis hyperendemic areas and the highest prevalences and intensities known. These scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. A study including DNA marker sequencing of fasciolids and lymnaeids, an experimental study of the life cycle in Uruguay, and a review of human fascioliasis in Uruguay, are performed. The characterization of Fasciola hepatica from cattle and horses of Uruguay included the complete sequences of the ribosomal DNA ITS-2 and ITS-1 and mitochondrial DNA cox1 and nad1. ITS-2, ITS-1, partial cox1 and rDNA 16S gene of mtDNA were used for lymnaeids. Results indicated that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. The life cycle and transmission features of F. hepatica by L. neotropica of Uruguay were studied under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. On this baseline, we reviewed the 95 human fascioliasis cases reported in Uruguay and analyzed the risk of human infection in front of future climate change estimations. The correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock into Uruguay allowed to understand the molecular diversity detected. Although Uruguayan L. neotropica is a highly efficient vector, its transmission capacity is markedly lower than that of Bolivian G. truncatula. This allows to understand the transmission and epidemiological differences between Andean highlands and non

  11. Legal capacity as a universal human right and a determinant of social status of people with mental disability

    OpenAIRE

    Marković Milan M.

    2012-01-01

    Adoption of the UN Convention on the Right of Persons with Disabilities (2006) brought about a core shift to how the international community and human rights law see and treat human disability in general. This paradigm shift materilizes itself in a number of provisions ranging from those which catalogue the proclaimed human rights as they are in the context of special implementation and protection of people with disabilities, to those that introduce a level of specificity in light of th...

  12. Dose-dependent adsorptive capacity of activated charcoal for gastrointestinal decontamination of a simulated paracetamol overdose in human volunteers

    DEFF Research Database (Denmark)

    Gude, Anne-Bolette Jill; Hoegberg, Lotte Christine Groth; Riis Angelo, Helle

    2010-01-01

    The amount of activated charcoal needed to treat drug overdoses has arbitrarily been set at a charcoal-drug ratio of 10:1. Recent in vitro studies have shown a larger adsorptive capacity for activated charcoal when used in a model of paracetamol overdose. In the present study, we investigated...... whether this reserve capacity exists in vivo. This is clinically relevant in cases of large overdoses or if the full standard dose of 50 g activated charcoal cannot be administered. We performed a randomized, cross-over study (n = 16). One hour after a standard breakfast, 50 mg/kg paracetamol...... was administered, followed 1 hr later by an activated charcoal-Water slurry containing 50 (control), 25 or 5 g activated charcoal. The areas under the serum concentration-time curve (AUC) for paracetamol were used to estimate the efficacy of each activated charcoal dose. The AUC of the 25-g dose was found...

  13. Systematic analysis of adaptations in aerobic capacity and submaximal energy metabolism provides a unique insight into determinants of human aerobic performance

    DEFF Research Database (Denmark)

    Vollaard, Niels B J; Constantin-Teodosiu, Dimitru; Fredriksson, Katarina

    2009-01-01

    It has not been established which physiological processes contribute to endurance training-related changes (Delta) in aerobic performance. For example, the relationship between intramuscular metabolic responses at the intensity used during training and improved human functional capacity has...... not been examined in a longitudinal study. In the present study we hypothesized that improvements in aerobic capacity (Vo(2max)) and metabolic control would combine equally to explain enhanced aerobic performance. Twenty-four sedentary males (24 +/- 2 yr; 1.81 +/- 0.08 m; 76.6 +/- 11.3 kg) undertook...... unrelated to the change in aerobic performance. The maximal parameters DeltaVe(max) and DeltaVeq(max) (DeltaVe/Vo(2max)) accounted for 64% of the variance in DeltaVo(2max) (P

  14. Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene

    Directory of Open Access Journals (Sweden)

    Viviana Meraviglia

    2018-03-01

    Full Text Available Caveolinopathies are a heterogeneous family of genetic pathologies arising from alterations of the caveolin-3 gene (CAV3, encoding for the isoform specifically constituting muscle caveolae. Here, by reprogramming peripheral blood mononuclear cells, we report the generation of induced pluripotent stem cells (iPSCs from three patients carrying the ΔYTT deletion, T78K and W101C missense mutations in caveolin-3. iPSCs displayed normal karyotypes and all the features of pluripotent stem cells in terms of morphology, specific marker expression and ability to differentiate in vitro into the three germ layers. These lines thus represent a human cellular model to study the molecular basis of caveolinopathies.Resource tableImage 1Unique stem cell lines identifierEURACi001-AEURACi002-AEURACi003-AAlternative names of stem cell linesB2CAV3 (EURACi001-AL1CAV3 (EURACi002-AN1CAV3 (EURACi003-AInstitutionInstitute for Biomedicine, Eurac ResearchContact information of distributorAlessandra Rossini (alessandra.rossini@eurac.eduType of cell linesiPSCsOriginHumanCell sourcePeripheral blood mononuclear cells (PBMCsMethod of reprogrammingElectroporation of episomal vectors (pCXLE hOCT3/4-shp53-F, pCXLE-hSK, and pCXLE-hULMultiline rationaleNon-isogenic cell lines obtained from patients with mutations in the same gene (CAV3Gene modificationNOType of modificationSpontaneous mutationsAssociated diseaseCaveolinopathiesGene/locusHeterozygous CAV3 c.Δ184–192 (EURACi001-AHeterozygous CAV3 c.303 TGG > TGC (EURACi002-AHeterozygous CAV3 c.233 ACG > AAG (EURACi003-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateJanuary 2016 (EURACi001-ASeptember 2016 (EURACi002-AMay 2016 (EURACi003-ACell line repository/bankN/AEthical approvalPeripheral blood was collected from patients after signing the informed consent provided by Cell Line and DNA Biobank from Patients Affected by Genetic Diseases, member of the

  15. The effect of aging and cardiorespiratory fitness on the lung diffusing capacity response to exercise in healthy humans.

    Science.gov (United States)

    Coffman, Kirsten E; Carlson, Alex R; Miller, Andrew D; Johnson, Bruce D; Taylor, Bryan J

    2017-06-01

    Aging is associated with deterioration in the structure and function of the pulmonary circulation. We characterized the lung diffusing capacity for carbon monoxide (DL CO ), alveolar-capillary membrane conductance (Dm CO ), and pulmonary-capillary blood volume (Vc) response to discontinuous incremental exercise at 25, 50, 75, and 90% of peak work (W peak ) in four groups: 1 ) Young [27 ± 3 yr, maximal oxygen consumption (V̇o 2max ): 110 ± 18% age predicted]; 2) Young Highly Fit (27 ± 3 yr, V̇o 2max : 147 ± 8% age predicted); 3 ) Old (69 ± 5 yr, V̇o 2max : 116 ± 13% age predicted); and 4 ) Old Highly Fit (65 ± 5 yr, V̇o 2max : 162 ± 18% age predicted). At rest and at 90% W peak , DL CO , Dm CO , and Vc were decreased with age. At 90% W peak , DL CO , Dm CO , and Vc were greater in Old Highly Fit vs. Old adults. The slope of the DL CO -cardiac output (Q̇) relationship from rest to end exercise at 90% W peak was not different between Young, Young Highly Fit, Old, and Old Highly Fit (1.35 vs. 1.44 vs. 1.10 vs. 1.35 ml CO ·mmHg -1 ·liter blood -1 , P = 0.388), with no evidence of a plateau in this relationship during exercise; this was also true for Dm CO -Q̇ and Vc-Q̇. V̇o 2max was positively correlated with 1 ) DL CO , Dm CO , and Vc at rest; and 2 ) the rest to end exercise change in DL CO , Dm CO , and Vc. In conclusion, these data suggest that despite the age-associated deterioration in the structure and function of the pulmonary circulation, expansion of the pulmonary capillary network does not become limited during exercise in healthy individuals regardless of age or cardiorespiratory fitness level. NEW & NOTEWORTHY Healthy aging is a crucial area of research. This article details how differences in age and cardiorespiratory fitness level affect lung diffusing capacity, particularly during high-intensity exercise. We conclude that highly fit older adults do not experience a limit in lung diffusing capacity during

  16. [Effect of Earth magnetic field on circadian rhythm of total antioxidant capacity of human saliva in the North].

    Science.gov (United States)

    Borisenkov, M F

    2007-01-01

    In the inhabitants of the North during increase of geomagnetic activity and during magnetic calm the decrease of amplitude of circadian rhythm of total antioxidant capacity of saliva is observed. The most favorable conditions to display the circadian rhythm are observed at Kp from 0,5 up to 2. The long residing in the North is connected to influence of irregularly varying geomagnetic activity causing disturbance of function of circadian and antioxidant systems that, probably, is one of the reasons of acceleration of process of aging at northerner and of higher risk of occurrence in them the age associated diseases.

  17. Enhanced Local Skeletal Muscle Oxidative Capacity and Microvascular Blood Flow Following 7-Day Ischemic Preconditioning in Healthy Humans

    Directory of Open Access Journals (Sweden)

    Owen Jeffries

    2018-05-01

    Full Text Available Ischemic preconditioning (IPC, which involves intermittent periods of ischemia followed by reperfusion, is an effective clinical intervention that reduces the risk of myocardial injury and confers ischemic tolerance to skeletal muscle. Repeated bouts of IPC have been shown to stimulate long-term changes vascular function, however, it is unclear what metabolic adaptations may occur locally in the muscle. Therefore, we investigated 7 days of bilateral lower limb IPC (4 × 5 min above limb occlusion pressure (220 mmHg; n = 10, or sham (20 mmHg; n = 10, on local muscle oxidative capacity and microvascular blood flow. Oxidative capacity was measured using near-infrared spectroscopy (NIRS during repeated short duration arterial occlusions (300 mmHg. Microvascular blood flow was assessed during the recovery from submaximal isometric plantar flexion exercises at 40 and 60% of maximal voluntary contraction (MVC. Following the intervention period, beyond the late phase of protection (72 h, muscle oxidative recovery kinetics were speeded by 13% (rate constant pre 2.89 ± 0.47 min-1 vs. post 3.32 ± 0.69 min-1; P < 0.05 and resting muscle oxygen consumption (mO2 was reduced by 16.4% (pre 0.39 ± 0.16%.s-1 vs. post 0.33 ± 0.14%.s-1; P < 0.05. During exercise, changes in deoxygenated hemoglobin (HHb from rest to steady state were reduced at 40 and 60% MVC (16 and 12%, respectively, P < 0.05 despite similar measures of total hemoglobin (tHb. At the cessation of exercise, the time constant for recovery in oxygenated hemoglobin (O2Hb was accelerated at 40 and 60% MVC (by 33 and 43%, respectively suggesting enhanced reoxygenation in the muscle. No changes were reported for systemic measures of resting heart rate or blood pressure. In conclusion, repeated bouts of IPC over 7 consecutive days increased skeletal muscle oxidative capacity and microvascular muscle blood flow. These findings are consistent with enhanced mitochondrial and vascular function following

  18. Modulation of DNA-induced damage and repair capacity in humans after dietary intervention with lutein-enriched fermented milk.

    Science.gov (United States)

    Herrero-Barbudo, Carmen; Soldevilla, Beatriz; Pérez-Sacristán, Belén; Blanco-Navarro, Inmaculada; Herrera, Mercedes; Granado-Lorencio, Fernando; Domínguez, Gemma

    2013-01-01

    Dietary factors provide protection against several forms of DNA damage. Additionally, consumer demand for natural products favours the development of bioactive food ingredients with health benefits. Lutein is a promising biologically active component in the food industry. The EFSA Panel on Dietetic Products, Nutrition and Allergies considers that protection from oxidative damage may be a beneficial physiological effect but that a cause and effect relationship has not been established. Thus, our aim was to evaluate the safety and potential functional effect of a lutein-enriched milk product using the Comet Assay in order to analyze the baseline, the induced DNA-damage and the repair capacity in the lymphocytes of 10 healthy donors before and after the intake of the mentioned product. Our data suggest that the regular consumption of lutein-enriched fermented milk results in a significant increase in serum lutein levels and this change is associated with an improvement in the resistance of DNA to damage and the capacity of DNA repair in lymphocytes. Our results also support the lack of a genotoxic effect at the doses supplied as well as the absence of interactions and side effects on other nutritional and biochemicals markers.

  19. Modulation of DNA-induced damage and repair capacity in humans after dietary intervention with lutein-enriched fermented milk.

    Directory of Open Access Journals (Sweden)

    Carmen Herrero-Barbudo

    Full Text Available Dietary factors provide protection against several forms of DNA damage. Additionally, consumer demand for natural products favours the development of bioactive food ingredients with health benefits. Lutein is a promising biologically active component in the food industry. The EFSA Panel on Dietetic Products, Nutrition and Allergies considers that protection from oxidative damage may be a beneficial physiological effect but that a cause and effect relationship has not been established. Thus, our aim was to evaluate the safety and potential functional effect of a lutein-enriched milk product using the Comet Assay in order to analyze the baseline, the induced DNA-damage and the repair capacity in the lymphocytes of 10 healthy donors before and after the intake of the mentioned product. Our data suggest that the regular consumption of lutein-enriched fermented milk results in a significant increase in serum lutein levels and this change is associated with an improvement in the resistance of DNA to damage and the capacity of DNA repair in lymphocytes. Our results also support the lack of a genotoxic effect at the doses supplied as well as the absence of interactions and side effects on other nutritional and biochemicals markers.

  20. Station Capacity

    DEFF Research Database (Denmark)

    Landex, Alex

    2011-01-01

    the probability of conflicts and the minimum headway times into account. The last method analyzes how optimal platform tracks are used by examining the arrival and departure pattern of the trains. The developed methods can either be used separately to analyze specific characteristics of the capacity of a station......Stations are often limiting the capacity of railway networks. This is due to extra need of tracks when trains stand still, trains turning around, and conflicting train routes. Although stations are often the capacity bottlenecks, most capacity analysis methods focus on open line capacity. Therefore...... for platform tracks and the probability that arriving trains will not get a platform track immediately at arrival. The third method is a scalable method that analyzes the conflicts in the switch zone(s). In its simplest stage, the method just analyzes the track layout while the more advanced stages also take...

  1. Uncertainty in adaptive capacity

    International Nuclear Information System (INIS)

    Neil Adger, W.; Vincent, K.

    2005-01-01

    The capacity to adapt is a critical element of the process of adaptation: it is the vector of resources that represent the asset base from which adaptation actions can be made. Adaptive capacity can in theory be identified and measured at various scales, from the individual to the nation. The assessment of uncertainty within such measures comes from the contested knowledge domain and theories surrounding the nature of the determinants of adaptive capacity and the human action of adaptation. While generic adaptive capacity at the national level, for example, is often postulated as being dependent on health, governance and political rights, and literacy, and economic well-being, the determinants of these variables at national levels are not widely understood. We outline the nature of this uncertainty for the major elements of adaptive capacity and illustrate these issues with the example of a social vulnerability index for countries in Africa. (authors)

  2. How honey bees carry pollen

    Science.gov (United States)

    Matherne, Marguerite E.; Anyanwu, Gabriel; Leavey, Jennifer K.; Hu, David L.

    2017-11-01

    Honey bees are the tanker of the skies, carrying thirty percent of their weight in pollen per foraging trip using specialized orifices on their body. How do they manage to hang onto those pesky pollen grains? In this experimental study, we investigate the adhesion force of pollen to the honeybee. To affix pollen to themselves, honey bees form a suspension of pollen in nectar, creating a putty-like pollen basket that is skewered by leg hairs. We use tensile tests to show that the viscous force of the pollen basket is more than ten times the honeybee's flight force. This work may provide inspiration for the design of robotic flying pollinators.

  3. Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells.

    Science.gov (United States)

    Giampieri, Francesca; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y; Mazzoni, Luca; Capocasa, Franco; Sabbadini, Silvia; Alvarez-Suarez, Josè M; Afrin, Sadia; Rosati, Carlo; Pandolfini, Tiziana; Molesini, Barbara; Sánchez-Sevilla, José F; Amaya, Iraida; Mezzetti, Bruno; Battino, Maurizio

    2018-01-24

    Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

  4. Effect of low dose UVB irradiation on the migratory properties and functional capacities of human skin dendritic cells

    NARCIS (Netherlands)

    Richters, C. D.; Reits, E. A.; van Pelt, A. M.; Hoekstra, M. J.; van Baare, J.; Du Pont, J. S.; Kamperdijk, E. W.

    1996-01-01

    We recently described the 'spontaneous' migration of skin dendritic cells out of human split skin during culture. Since newly infiltrating cells from the circulation are excluded, this in vitro model is very suitable for studying the effect of UVB irradiation on the migratory properties, phenotype

  5. Differences in Cartilage-Forming Capacity of Expanded Human Chondrocytes From Ear and Nose and Their Gene Expression Profiles

    NARCIS (Netherlands)

    Hellingman, C.A.; Verwiel, E.T.P.; Slagt, I.; Koevoet, W.; Poublon, R.M.L.; Nolst-Trenite, G.J.; de Jong, R.J.B.; Jahr, H.; van Osch, G.J.V.M.

    2011-01-01

    The aim of this study was to evaluate the potential of culture-expanded human auricular and nasoseptal chondrocytes as cell source for regeneration of stable cartilage and to analyze the differences in gene expression profile of expanded chondrocytes from these specific locations. Auricular

  6. Differences in cartilage-forming capacity of expanded human chondrocytes from ear and nose and their gene expression profiles

    NARCIS (Netherlands)

    Hellingman, Catharine A.; Verwiel, Eugène T. P.; Slagt, Inez; Koevoet, Wendy; Poublon, René M. L.; Nolst-Trenité, Gilbert J.; Baatenburg de Jong, Robert J.; Jahr, Holger; van Osch, Gerjo J. V. M.

    2011-01-01

    The aim of this study was to evaluate the potential of culture-expanded human auricular and nasoseptal chondrocytes as cell source for regeneration of stable cartilage and to analyze the differences in gene expression profile of expanded chondrocytes from these specific locations. Auricular

  7. How comparative psychology can shed light on human evolution: Response to Beran et al.'s discussion of "Cognitive capacities for cooking in chimpanzees".

    Science.gov (United States)

    Rosati, Alexandra G; Warneken, Felix

    2016-06-01

    We recently reported a study (Warneken & Rosati Proceedings of the Royal Society B, 282, 20150229, 2015) examining whether chimpanzees possess several cognitive capacities that are critical to engage in cooking. In a subsequent commentary, Beran, Hopper, de Waal, Sayers, and Brosnan Learning & Behavior (2015) asserted that our paper has several flaws. Their commentary (1) critiques some aspects of our methodology and argues that our work does not constitute evidence that chimpanzees can actually cook; (2) claims that these results are old news, as previous work had already demonstrated that chimpanzees possess most or all of these capacities; and, finally, (3) argues that comparative psychological studies of chimpanzees cannot adequately address questions about human evolution, anyway. However, their critique of the premise of our study simply reiterates several points we made in the original paper. To quote ourselves: "As chimpanzees neither control fire nor cook food in their natural behavior, these experiments therefore focus not on whether chimpanzees can actually cook food, but rather whether they can apply their cognitive skills to novel problems that emulate cooking" (Warneken & Rosati Proceedings of the Royal Society B, 282, 20150229, 2015, p. 2). Furthermore, the methodological issues they raise are standard points about psychological research with animals-many of which were addressed synthetically across our 9 experiments, or else are orthogonal to our claims. Finally, we argue that comparative studies of extant apes (and other nonhuman species) are a powerful and indispensable method for understanding human cognitive evolution.

  8. Dietary fibers from mushroom Sclerotia: 2. In vitro mineral binding capacity under sequential simulated physiological conditions of the human gastrointestinal tract.

    Science.gov (United States)

    Wong, Ka-Hing; Cheung, Peter C K

    2005-11-30

    The in vitro mineral binding capacity of three novel dietary fibers (DFs) prepared from mushroom sclerotia, namely, Pleurotus tuber-regium, Polyporous rhinocerus, and Wolfiporia cocos, to Ca, Mg, Cu, Fe, and Zn under sequential simulated physiological conditions of the human stomach, small intestine, and colon was investigated and compared. Apart from releasing most of their endogenous Ca (ranged from 96.9 to 97.9% removal) and Mg (ranged from 95.9 to 96.7% removal), simulated physiological conditions of the stomach also attenuated the possible adverse binding effect of the three sclerotial DFs to the exogenous minerals by lowering their cation-exchange capacity (ranged from 20.8 to 32.3%) and removing a substantial amount of their potential mineral chelators including protein (ranged from 16.2 to 37.8%) and phytate (ranged from 58.5 to 64.2%). The in vitro mineral binding capacity of the three sclerotial DF under simulated physiological conditions of small intestine was found to be low, especially for Ca (ranged from 4.79 to 5.91% binding) and Mg (ranged from 3.16 to 4.18% binding), and was highly correlated (r > 0.97) with their residual protein contents. Under simulated physiological conditions of the colon with slightly acidic pH (5.80), only bound Ca was readily released (ranged from 34.2 to 72.3% releasing) from the three sclerotial DFs, and their potential enhancing effect on passive Ca absorption in the human large intestine was also discussed.

  9. Evaluation of the radioinduced damage, repair capacity and cell death on human tumorigenic (T-47D and MCF-7) and nontumorigenic (MCF-10) cell lines of breast

    International Nuclear Information System (INIS)

    Valdoge, Flavia Gomes Silva

    2008-01-01

    Breast cancer is one of the most common malignancies that account women, representing about one in three of all female neoplasm. Approximately, 90% of cases are considered sporadic, attributed to somatic events and about 10% have a family history and this only 4 - 5 % is due to hereditary factors. In the clinic, ionizing radiation is a major tool utilized in the control of tumour growth, besides surgery and chemotherapy. There is, however, little information concerning cellular response to the action of ionizing radiation in the target cells, i.e., cell lines originating from breast cancer. The present study proposed to analyze the radiosensitivity of the human tumorigenic (T-47D and MCF-7) and non tumorigenic (MCF-10) cell lines, originating from breast and submitted to various doses (0.5 to 30 Gy) of 60 Co rays (0.72 - 1.50 Gy/min). For this purpose, DNA radioinduced damage, repair capacity and cell death were utilized as parameters of radiosensitivity by micronucleus, single cell gel electrophoresis (Comet assay) and cell viability techniques. The data obtained showed that tumorigenic cell lines were more radiosensitive than non tumorigenic breast cells in all assays here utilized. The T-47D cell line was presenting the highest amount of radioinduced damage, a more accelerated proliferation rate and a higher rate of cell death. The three cell lines presented a relatively efficient repair capacity, since one hour after the irradiation all of them showed a considerable reduction of radioinduced damage. The techniques employed showed to be secure, sensitive and reproducible, allowing to quantify and evaluate DNA damage, repair capacity and cell death in the three human breast cell lines. (author)

  10. DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis

    Science.gov (United States)

    Gayo, Valeria; Sanchis, Jaime; Artigas, Patricio; Khoubbane, Messaoud; Birriel, Soledad; Mas-Coma, Santiago

    2017-01-01

    Background Fascioliasis is a pathogenic disease transmitted by lymnaeid snails and recently emerging in humans, in part due to effects of climate changes, anthropogenic environment modifications, import/export and movements of livestock. South America is the continent presenting more human fascioliasis hyperendemic areas and the highest prevalences and intensities known. These scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. A study including DNA marker sequencing of fasciolids and lymnaeids, an experimental study of the life cycle in Uruguay, and a review of human fascioliasis in Uruguay, are performed. Methodology/Principal findings The characterization of Fasciola hepatica from cattle and horses of Uruguay included the complete sequences of the ribosomal DNA ITS-2 and ITS-1 and mitochondrial DNA cox1 and nad1. ITS-2, ITS-1, partial cox1 and rDNA 16S gene of mtDNA were used for lymnaeids. Results indicated that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. The life cycle and transmission features of F. hepatica by L. neotropica of Uruguay were studied under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. On this baseline, we reviewed the 95 human fascioliasis cases reported in Uruguay and analyzed the risk of human infection in front of future climate change estimations. Conclusions/Significance The correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock into Uruguay allowed to understand the molecular diversity detected. Although Uruguayan L. neotropica is a highly efficient vector, its transmission capacity is markedly lower than that of Bolivian G. truncatula. This allows to understand the transmission and

  11. Increased cross-bridge recruitment contributes to transient increase in force generation beyond maximal capacity in human myocardium.

    Science.gov (United States)

    Milani-Nejad, Nima; Chung, Jae-Hoon; Canan, Benjamin D; Fedorov, Vadim V; Whitson, Bryan A; Kilic, Ahmet; Mohler, Peter J; Janssen, Paul M L

    2018-01-01

    Cross-bridge attachment allows force generation to occur, and rate of tension redevelopment (k tr ) is a commonly used index of cross-bridge cycling rate. Tension overshoots have been observed briefly after a slack-restretch k tr maneuver in various species of animal models and humans. In this study, we set out to determine the properties of these overshoots and their possible underlying mechanism. Utilizing human cardiac trabeculae, we have found that tension overshoots are temperature-dependent and that they do not occur at resting states. In addition, we have found that myosin cross-bridge cycle is vital to these overshoots as inhibition of the cycle results in the blunting of the overshoots and the magnitude of the overshoots are dependent on the level of myofilament activation. Lastly, we show that the number of cross-bridges transiently increase during tension overshoots. These findings lead us to conclude that tension overshoots are likely due to a transient enhancement of the recruitment of myosin heads into the cross-bridge cycling, regulated by the myocardium, and with potential physiological significance in determining cardiac output. We show that isolated human myocardium is capable of transiently increasing its maximal force generation capability by increasing cross-bridge recruitment following slack-restretch maneuver. This process can potentially have important implications and significance in cardiac contraction in vivo. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Possible Climate Change/Variability and Human Impacts, Vulnerability of African Drought Prone Regions, its Water Resources and Capacity Building

    Science.gov (United States)

    Gan, T. Y. Y.; Qin, X.; Ito, M.; Hülsmann, S.; Xixi, L.; Liong, S. Y.; Disse, M.; Koivusalo, H. J.

    2017-12-01

    This review article discusses the climate, water resources and historical droughts of Africa, drought indices, vulnerability, impact of global warming and landuse to drought-prone regions in West, Southern, and Greater Horn of Africa, which have suffered recurrent severe droughts in the past. Recent studies detected warming and drying trends in Africa since the mid-20th century. Based on the 4th Assessment Report of the Intergovernmental Panel of Climate Change, and that of the 5th Coupled Model Intercomparison Project (CMIP5), both northern and southern Africa are projected to experience drying such as decreasing precipitation, runoff and soil moisture in the 21st Century and could become more vulnerable to impact of droughts. The daily maximum temperature is projected to increase up to 8oC (RCP8.5 of CMIP5), precipitation indices such as total wet day precipitation (PRCPTOT) and heavy precipitation days (R10mm) could decrease, while warm spell duration (WSDI) and consecutive dry days (CDD) could increase. Uncertainties of the above long-term projections, teleconnections to climate anomalies such as ENSO and Madden Julian Oscillation which could also affect water resources of Africa, and capacity building in terms of physical infrastructure and non-structural solutions, are also discussed. Given traditional climate and hydrologic data observed in Africa are generally limited, satellite data should also be exploited to fill in the data gap for Africa in future.

  13. Metabolism: The Physiological Power-Generating Process: A History of Methods to Test Human Beings' \\"Vital Capacity\\" [Retrospectroscope].

    Science.gov (United States)

    Johnston, Richard; Valentinuzzi, Max E

    2016-01-01

    A previous "Retrospectroscope" note, published early in 2014, dealt with spirometry: it described many apparatuses used to measure the volume of inhaled and exhaled air that results from breathing [1]. Such machines, when adequately modified, are also able to measure the rate at which work is produced (specifically by an animal or a human being). Metabolism in that sense is the term used by physiologists and physicians, a word that in Greek, metabolismos, means "change" or "overthrow," in the sense of breaking down material, as in burning some stuff.

  14. Antioxidant capacity and radical scavenging effect of polyphenol rich Mallotus philippenensis fruit extract on human erythrocytes: an in vitro study.

    Science.gov (United States)

    Gangwar, Mayank; Gautam, Manish Kumar; Sharma, Amit Kumar; Tripathi, Yamini B; Goel, R K; Nath, Gopal

    2014-01-01

    Mallotus philippinensis is an important source of molecules with strong antioxidant activity widely used medicinal plant. Previous studies have highlighted their anticestodal, antibacterial, wound healing activities, and so forth. So, present investigation was designed to evaluate the total antioxidant activity and radical scavenging effect of 50% ethanol fruit glandular hair extract (MPE) and its role on Human Erythrocytes. MPE was tested for phytochemical test followed by its HPLC analysis. Standard antioxidant assays like DPPH, ABTS, hydroxyl, superoxide radical, nitric oxide, and lipid peroxidation assay were determined along with total phenolic and flavonoids content. Results showed that MPE contains the presence of various phytochemicals, with high total phenolic and flavonoid content. HPLC analysis showed the presence of rottlerin, a polyphenolic compound in a very rich quantity. MPE exhibits significant strong scavenging activity on DPPH and ABTS assay. Reducing power showed dose dependent increase in concentration absorption compared to standard, Quercetin. Superoxide, hydroxyl radical, lipid peroxidation, nitric oxide assay showed a comparable scavenging activity compared to its standard. Our finding further provides evidence that Mallotus fruit extract is a potential natural source of antioxidants which have a protective role on human Erythrocytes exhibiting minimum hemolytic activity and this justified its uses in folklore medicines.

  15. Rotator cuff tear state modulates self-renewal and differentiation capacity of human skeletal muscle progenitor cells.

    Science.gov (United States)

    Thomas, Kelsey A; Gibbons, Michael C; Lane, John G; Singh, Anshuman; Ward, Samuel R; Engler, Adam J

    2017-08-01

    Full thickness rotator cuff tendon (RCT) tears have long-term effects on RC muscle atrophy and fatty infiltration, with lasting damage even after surgical tendon repair. Skeletal muscle progenitor cells (SMPs) are critical for muscle repair in response to injury, but the inability of RC muscles to recover from chronic RCT tear indicates possible deficits in repair mechanisms. Here we investigated if muscle injury state was a crucial factor during human SMP expansion and differentiation ex vivo. SMPs were isolated from muscles in patients with no, partial-thickness (PT), or full-thickness (FT) RCT tears. Despite using growth factors, physiological niche stiffness, and muscle-mimetic extracellular matrix (ECM) proteins, we found that SMPs isolated from human RC muscle with RCT tears proliferated slower but fused into myosin heavy chain (MHC)-positive myotubes at higher rates than SMPs from untorn RCTs. Proteomic analysis of RC muscle tissue revealed shifts in muscle composition with pathology, as muscle from massive RCT tears had increased ECM deposition compared with no tear RC muscle. Together these data imply that the remodeled niche in a torn RCT primes SMPs not for expansion but for differentiation, thus limiting longer-term self-renewal necessary for regeneration after surgical repair. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1816-1823, 2017. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

  16. 基于游客感知的江南园林旅游容量探讨--以扬州个园为例%A Discussion of Tourism Carrying Capacity of JiangNan Gardens---Take Yangzhou Ge Garden for Instance

    Institute of Scientific and Technical Information of China (English)

    王彦龙

    2014-01-01

    通过访谈调研和问卷调查两种形式,对近年来扬州个园景区的基于游客感知的旅游容量的具体情况进行了实际评估和探讨研究,得出一些关于个园旅游容量的实际问题,找出影响个园旅游容量的一些主客观因素,并有针对性地提出可行性措施和建议。%This paper highlights the practical evaluation and argumentative survey of the specific condition , which based on the tourists ’ perceptional capacity of tourism through the interview survey and questionnaire survey for Yangzhou Ge Garden in recent years .Moreover , the survey gets some conclusions about the practical question of capacity of tourism .In the meanwhile , this paper also found out some subjective factors which can affect the capac-ity of the tourism and suggests some practical measures and suggestion to the specific aspects .

  17. [Differences in human evoked visual potentials depending on the capacity for the self-regulation of their parameters by feedback].

    Science.gov (United States)

    Mnatsakanian, E V

    1997-01-01

    The individual characteristics of the VEP spatio-temporal patterns were studied depending on a capability of a subject for VEP biofeedback conditioning. Thirty two healthy adult volunteers had to modify their N100-P200 VEP component (Cz derivation) within a selected time window in order to increase the number of trials in which the amplitude of the segment from the beginning of the sample to the positive peak exceeded a definite threshold. In addition to traditional visual assessment of topographical maps, the statistical analysis of multichannel VEPs and component analysis for Cz, O1, and O2 were carried out for each discrete time sample. The differences were observed in the spatio-temporal VEP patterns for successful and unsuccessful performance. In the background sessions, the central and occipital P200 components were much the same in the subjects which succeeded in task performance, whereas the central component was noticeably higher than the occipital one in the subjects who were unsuccessful in biofeedback control. During successful performance, there was a reduction of the occipital P200. Thus, the relationships of VEP components in the central and occipital derivations can predict successful biofeedback conditioning and may reflect the prevalence of verbal and non-verbal thinking.

  18. Early interferon-γ production in human lymphocyte subsets in response to nontyphoidal Salmonella demonstrates inherent capacity in innate cells.

    Directory of Open Access Journals (Sweden)

    Tonney S Nyirenda

    2010-10-01

    Full Text Available Nontyphoidal Salmonellae frequently cause life-threatening bacteremia in sub-Saharan Africa. Young children and HIV-infected adults are particularly susceptible. High case-fatality rates and increasing antibiotic resistance require new approaches to the management of this disease. Impaired cellular immunity caused by defects in the T helper 1 pathway lead to intracellular disease with Salmonella that can be countered by IFNγ administration. This report identifies the lymphocyte subsets that produce IFNγ early in Salmonella infection.Intracellular cytokine staining was used to identify IFNγ production in blood lymphocyte subsets of ten healthy adults with antibodies to Salmonella (as evidence of immunity to Salmonella, in response to stimulation with live and heat-killed preparations of the D23580 invasive African isolate of Salmonella Typhimurium. The absolute number of IFNγ-producing cells in innate, innate-like and adaptive lymphocyte subpopulations was determined.Early IFNγ production was found in the innate/innate-like lymphocyte subsets: γδ-T cells, NK cells and NK-like T cells. Significantly higher percentages of such cells produced IFNγ compared to adaptive αβ-T cells (Student's t test, P<0.001 and ≤0.02 for each innate subset compared, respectively, with CD4(+- and CD8(+-T cells. The absolute numbers of IFNγ-producing cells showed similar differences. The proportion of IFNγ-producing γδ-T cells, but not other lymphocytes, was significantly higher when stimulated with live compared with heat-killed bacteria (P<0.0001.Our findings indicate an inherent capacity of innate/innate-like lymphocyte subsets to produce IFNγ early in the response to Salmonella infection. This may serve to control intracellular infection and reduce the threat of extracellular spread of disease with bacteremia which becomes life-threatening in the absence of protective antibody. These innate cells may also help mitigate against the effect on IFN

  19. Influence of O-methylated metabolite penetrating the blood-brain barrier to estimation of dopamine synthesis capacity in human L-[β-(11)C]DOPA PET.

    Science.gov (United States)

    Matsubara, Keisuke; Ikoma, Yoko; Okada, Maki; Ibaraki, Masanobu; Suhara, Tetsuya; Kinoshita, Toshibumi; Ito, Hiroshi

    2014-02-01

    O-methyl metabolite (L-[β-(11)C]OMD) of (11)C-labeled L-3,4-dihydroxyphenylalanine (L-[β-(11)C]DOPA) can penetrate into brain tissue through the blood-brain barrier, and can complicate the estimation of dopamine synthesis capacity by positron emission tomography (PET) study with L-[β-(11)C]DOPA. We evaluated the impact of L-[β-(11)C]OMD on the estimation of the dopamine synthesis capacity in a human L-[β-(11)C]DOPA PET study. The metabolite correction with mathematical modeling of L-[β-(11)C]OMD kinetics in a reference region without decarboxylation and further metabolism, proposed by a previous [(18)F]FDOPA PET study, were implemented to estimate radioactivity of tissue L-[β-(11)C]OMD in 10 normal volunteers. The component of L-[β-(11)C]OMD in tissue time-activity curves (TACs) in 10 regions were subtracted by the estimated radioactivity of L-[β-(11)C]OMD. To evaluate the influence of omitting blood sampling and metabolite correction, relative dopamine synthesis rate (kref) was estimated by Gjedde-Patlak analysis with reference tissue input function, as well as the net dopamine synthesis rate (Ki) by Gjedde-Patlak analysis with the arterial input function and TAC without and with metabolite correction. Overestimation of Ki was observed without metabolite correction. However, the kref and Ki with metabolite correction were significantly correlated. These data suggest that the influence of L-[β-(11)C]OMD is minimal for the estimation of kref as dopamine synthesis capacity.

  20. E3L and F1L Gene Functions Modulate the Protective Capacity of Modified Vaccinia Virus Ankara Immunization in Murine Model of Human Smallpox

    Directory of Open Access Journals (Sweden)

    Asisa Volz

    2018-01-01

    Full Text Available The highly attenuated Modified Vaccinia virus Ankara (MVA lacks most of the known vaccinia virus (VACV virulence and immune evasion genes. Today MVA can serve as a safety-tested next-generation smallpox vaccine. Yet, we still need to learn about regulatory gene functions preserved in the MVA genome, such as the apoptosis inhibitor genes F1L and E3L. Here, we tested MVA vaccine preparations on the basis of the deletion mutant viruses MVA-ΔF1L and MVA-ΔE3L for efficacy against ectromelia virus (ECTV challenge infections in mice. In non-permissive human tissue culture the MVA deletion mutant viruses produced reduced levels of the VACV envelope antigen B5. Upon mousepox challenge at three weeks after vaccination, MVA-ΔF1L and MVA-ΔE3L exhibited reduced protective capacity in comparison to wildtype MVA. Surprisingly, however, all vaccines proved equally protective against a lethal ECTV infection at two days after vaccination. Accordingly, the deletion mutant MVA vaccines induced high levels of virus-specific CD8+ T cells previously shown to be essential for rapidly protective MVA vaccination. These results suggest that inactivation of the anti-apoptotic genes F1L or E3L modulates the protective capacity of MVA vaccination most likely through the induction of distinct orthopoxvirus specific immunity in the absence of these viral regulatory proteins.

  1. Effect of radiation on the expression of E-cadherin and α-catenin and invasive capacity in human lung cancer cell line in vitro

    International Nuclear Information System (INIS)

    Akimoto, Tetsuo; Mitsuhashi, Norio; Saito, Yoshihiro; Ebara, Takeshi; Niibe, Hideo

    1998-01-01

    Purpose: To investigate the effect of radiation on E-cadherin and α-catenin expression in a human lung cancer cell line, and also evaluate invasive capacity in the membrane invasion culture system using the Boyden Chamber. Materials and Methods: The immunoblot and immunofluorescence analyses were performed using the human lung cancer cell line A549 to examine altered expression of E-cadherin and α-catenin after irradiation. We also compared invasive capacity of untreated cells with that of irradiated cells. Results: Immunoblot analysis revealed that the expression of E-cadherin increased after irradiation. In a time-course analysis, the expression was increased 6 h after irradiation with 10 Gy and reached its peak level at 24 h, being 2.3 times the control value, whereas expression at 1 and 3 h after irradiation was almost equivalent to that of the control. A slight increase in expression was observed after irradiation of 2 Gy and the expression reached peak levels after 5 Gy. After fractionated irradiation, the increase in expression of both E-cadherin and α-catenin was observed, and the alteration of α-catenin was more prominent than that after a single irradiation of the same total dose. In the immunofluorescence study for E-cadherin antibody analyzed by confocal laser scanning microscopy, increased intensity in irradiated cells produced as a nondisrupted and continuous line at cell-cell contact sites. In an invasive assay, the number of migrated cells in irradiated cells after a dose of 5 and 10 Gy was reduced significantly compared to untreated cells. Conclusion: The results indicate that irradiation of A549 increased the expression of E-cadherin, possibly preserving their functional property

  2. Expression of the human isoform of glutamate dehydrogenase, hGDH2, augments TCA cycle capacity and oxidative metabolism of glutamate during glucose deprivation in astrocytes.

    Science.gov (United States)

    Nissen, Jakob D; Lykke, Kasper; Bryk, Jaroslaw; Stridh, Malin H; Zaganas, Ioannis; Skytt, Dorte M; Schousboe, Arne; Bak, Lasse K; Enard, Wolfgang; Pääbo, Svante; Waagepetersen, Helle S

    2017-03-01

    A key enzyme in brain glutamate homeostasis is glutamate dehydrogenase (GDH) which links carbohydrate and amino acid metabolism mediating glutamate degradation to CO 2 and expanding tricarboxylic acid (TCA) cycle capacity with intermediates, i.e. anaplerosis. Humans express two GDH isoforms, GDH1 and 2, whereas most other mammals express only GDH1. hGDH1 is widely expressed in human brain while hGDH2 is confined to astrocytes. The two isoforms display different enzymatic properties and the nature of these supports that hGDH2 expression in astrocytes potentially increases glutamate oxidation and supports the TCA cycle during energy-demanding processes such as high intensity glutamatergic signaling. However, little is known about how expression of hGDH2 affects the handling of glutamate and TCA cycle metabolism in astrocytes. Therefore, we cultured astrocytes from cerebral cortical tissue of hGDH2-expressing transgenic mice. We measured glutamate uptake and metabolism using [ 3 H]glutamate, while the effect on metabolic pathways of glutamate and glucose was evaluated by use of 13 C and 14 C substrates and analysis by mass spectrometry and determination of radioactively labeled metabolites including CO 2 , respectively. We conclude that hGDH2 expression increases capacity for uptake and oxidative metabolism of glutamate, particularly during increased workload and aglycemia. Additionally, hGDH2 expression increased utilization of branched-chain amino acids (BCAA) during aglycemia and caused a general decrease in oxidative glucose metabolism. We speculate, that expression of hGDH2 allows astrocytes to spare glucose and utilize BCAAs during substrate shortages. These findings support the proposed role of hGDH2 in astrocytes as an important fail-safe during situations of intense glutamatergic activity. GLIA 2017;65:474-488. © 2016 Wiley Periodicals, Inc.

  3. Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin

    Directory of Open Access Journals (Sweden)

    Kashanchi Fatah

    2006-01-01

    Full Text Available Abstract Background The early events of the HIV-1 life cycle include entry of the viral core into target cell, assembly of the reverse transcription complex (RTCs performing reverse transcription, its transformation into integration-competent complexes called pre-integration complexes (PICs, trafficking of complexes into the nucleus, and finally integration of the viral DNA into chromatin. Molecular details and temporal organization of these processes remain among the least investigated and most controversial problems in the biology of HIV. Results To quantitatively evaluate maturation and nuclear translocation of the HIV-1 RTCs, nucleoprotein complexes isolated from the nucleus (nRTC and cytoplasm (cRTC of HeLa cells infected with MLV Env-pseudotyped HIV-1 were analyzed by real-time PCR. While most complexes completed reverse transcription in the cytoplasm, some got into the nucleus before completing DNA synthesis. The HIV-specific RNA complexes could get into the nucleus when reverse transcription was blocked by reverse transcriptase inhibitor, although nuclear import of RNA complexes was less efficient than of DNA-containing RTCs. Analysis of the RTC nuclear import in synchronized cells infected in the G2/M phase of the cell cycle showed enrichment in the nuclei of RTCs containing incomplete HIV-1 DNA compared to non-synchronized cells, where RTCs with complete reverse transcripts prevailed. Immunoprecipitation assays identified viral proteins IN, Vpr, MA, and cellular Ini1 and PML associated with both cRTCs and nRTCs, whereas CA was detected only in cRTCs and RT was diminished in nRTCs. Cytoplasmic maturation of the complexes was associated with increased immunoreactivity with anti-Vpr and anti-IN antibodies, and decreased reactivity with antibodies to RT. Both cRTCs and nRTCs carried out endogenous reverse transcription reaction in vitro. In contrast to cRTCs, in vitro completion of reverse transcription in nRTCs did not increase their

  4. Bioengineering a human plasma-based epidermal substitute with efficient grafting capacity and high content in clonogenic cells.

    Science.gov (United States)

    Alexaline, Maia M; Trouillas, Marina; Nivet, Muriel; Bourreau, Emilie; Leclerc, Thomas; Duhamel, Patrick; Martin, Michele T; Doucet, Christelle; Fortunel, Nicolas O; Lataillade, Jean-Jacques

    2015-06-01

    Cultured epithelial autografts (CEAs) produced from a small, healthy skin biopsy represent a lifesaving surgical technique in cases of full-thickness skin burn covering >50% of total body surface area. CEAs also present numerous drawbacks, among them the use of animal proteins and cells, the high fragility of keratinocyte sheets, and the immaturity of the dermal-epidermal junction, leading to heavy cosmetic and functional sequelae. To overcome these weaknesses, we developed a human plasma-based epidermal substitute (hPBES) for epidermal coverage in cases of massive burn, as an alternative to traditional CEA, and set up critical quality controls for preclinical and clinical studies. In this study, phenotypical analyses in conjunction with functional assays (clonal analysis, long-term culture, or in vivo graft) showed that our new substitute fulfills the biological requirements for epidermal regeneration. hPBES keratinocytes showed high potential for cell proliferation and subsequent differentiation similar to healthy skin compared with a well-known reference material, as ascertained by a combination of quality controls. This work highlights the importance of integrating relevant multiparameter quality controls into the bioengineering of new skin substitutes before they reach clinical development. This work involves the development of a new bioengineered epidermal substitute with pertinent functional quality controls. The novelty of this work is based on this quality approach. ©AlphaMed Press.

  5. A human hemi-cornea model for eye irritation testing: quality control of production, reliability and predictive capacity.

    Science.gov (United States)

    Engelke, M; Zorn-Kruppa, M; Gabel, D; Reisinger, K; Rusche, B; Mewes, K R

    2013-02-01

    We have developed a 3-dimensional human hemi-cornea which comprises an immortalized epithelial cell line and keratocytes embedded in a collagen stroma. In the present study, we have used MTT reduction of the whole tissue to clarify whether the production of this complex 3-D-model is transferable into other laboratories and whether these tissues can be constructed reproducibly. Our results demonstrate the reproducible production of the hemi-cornea model according to standard operation procedures using 15 independent batches of reconstructed hemi-cornea models in two independent laboratories each. Furthermore, the hemi-cornea tissues have been treated with 20 chemicals of different eye-irritating potential under blind conditions to assess the performance and limitations of our test system comparing three different prediction models. The most suitable prediction model revealed an overall in vitro-in vivo concordance of 80% and 70% in the participating laboratories, respectively, and an inter-laboratory concordance of 80%. Sensitivity of the test was 77% and specificity was between 57% and 86% to discriminate classified from non-classified chemicals. We conclude that additional physiologically relevant endpoints in both epithelium and stroma have to be developed for the reliable prediction of all GHS classes of eye irritation in one stand alone test system. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. Encapsulation of metalloporphyrins improves their capacity to block the viability of the human malaria parasite Plasmodium falciparum.

    Science.gov (United States)

    Alves, Eduardo; Iglesias, Bernardo A; Deda, Daiana K; Budu, Alexandre; Matias, Tiago A; Bueno, Vânia B; Maluf, Fernando V; Guido, Rafael V C; Oliva, Glaucius; Catalani, Luiz H; Araki, Koiti; Garcia, Celia R S

    2015-02-01

    Several synthetic metallated protoporphyrins (M-PPIX) were tested for their ability to block the cell cycle of the lethal human malaria parasite Plasmodium falciparum. After encapsulating the porphyrin derivatives in micro- and nanocapsules of marine atelocollagen, their effects on cultures of red blood cells infected (RBC) with P. falciparum were verified. RBCs infected with synchronized P. falciparum incubated for 48 h showed a toxic effect over a micromolar range. Strikingly, the IC50 of encapsulated metalloporphyrins reached nanomolar concentrations, where Zn-PPIX showed the best antimalarial effect, with an IC50=330 nM. This value is an 80-fold increase in the antimalarial activity compared to the antimalarial effect of non-encapsulated Zn-PPIX. These findings reveal that the incubation of P. falciparum infected-RBCs with 20 μM Zn-PPIX reduced the size of hemozoin crystal by 34%, whereas a 28% reduction was noticed with chloroquine, confirming the importance of heme detoxification pathway in drug therapy. In this study, synthetic metalloporphyrins were tested as therapeutics that target Plasmodium falciparum. The IC50 of encapsulated metalloporphyrins was found to be in the nanomolar concentration range, with encapsulated Zn-PPIX showing an 80-fold increase in its antimalarial activity compared to the non-encapsulated form. Copyright © 2015. Published by Elsevier Inc.

  7. Markets and Institutional Capacity

    DEFF Research Database (Denmark)

    Ingemann, Jan Holm

    2010-01-01

    Adequate explanations concerning the introduction of production and consumption of organic food in Denmark imply the necessity to engage a certain understanding of markets. Markets should subsequently not be seen as entities nor places but as complex relations between human actors. Further......, the establishment, maintenance and development of markets are depending on the capacity of the actors to enter into continuous and enhancing interplay....

  8. URG4/URGCP enhances the angiogenic capacity of human hepatocellular carcinoma cells in vitro via activation of the NF-κB signaling pathway

    International Nuclear Information System (INIS)

    Xing, Sizhong; Zhang, Bing; Hua, Ruixi; Tai, William Chi-shing; Zeng, Zhirong; Xie, Binhui; Huang, Chenghui; Xue, Jisu; Xiong, Shiqiu; Yang, Jianyong; Liu, Side; Li, Heping

    2015-01-01

    Angiogenesis is essential for tumor growth. Hepatocellular carcinoma (HCC) is characterized by hypervascularity; high levels of angiogenesis are associated with poor prognosis and a highly invasive phenotype in HCC. Up-regulated gene-4 (URG4), also known as upregulator of cell proliferation (URGCP), is overexpressed in multiple tumor types and has been suggested to act as an oncogene. This study aimed to elucidate the effect of URG4/URGCP on the angiogenic capacity of HCC cells in vitro. Expression of URG4/URGCP in HCC cell lines and normal liver epithelial cell lines was examined by Western blotting and quantitative real-time PCR. URG4/URGCP was stably overexpressed or transiently knocked down using a shRNA in two HCC cell lines. The human umbilical vein endothelial cell (HUVEC) tubule formation and Transwell migration assays and chicken chorioallantoic membrane (CAM) assay were used to examine the angiogenic capacity of conditioned media from URG4/URGCP-overexpressing and knockdown cells. A luciferase reporter assay was used to examine the transcriptional activity of nuclear factor kappa – light – chain - enhancer of activated B cells (NF-κB). NF-κB was inhibited by overexpressing degradation-resistant mutant inhibitor of κB (IκB)-α. Expression of vascular endothelial growth factor C (VEGFC), tumor necrosis factor-α (TNFα), interleukin (IL)-6, IL-8 and v-myc avian myelocytomatosis viral oncogene homolog (MYC) were examined by quantitative real-time PCR; VEGFC protein expression was analyzed using an ELISA. URG4/URGCP protein and mRNA expression were significantly upregulated in HCC cell lines. Overexpressing URG4/URGCP enhanced - while silencing URG4/URGCP decreased - the capacity of HCC cell conditioned media to induce HUVEC tubule formation and migration and neovascularization in the CAM assay. Furthermore, overexpressing URG4/URGCP increased - whereas knockdown of URG4/URGCP decreased - VEGFC expression, NF-κB transcriptional activity, the levels

  9. Human mesenchymal stem cells promote CD34+ hematopoietic stem cell proliferation with preserved red blood cell differentiation capacity.

    Science.gov (United States)

    Lau, Show Xuan; Leong, Yin Yee; Ng, Wai Hoe; Ng, Albert Wee Po; Ismail, Ida Shazrina; Yusoff, Narazah Mohd; Ramasamy, Rajesh; Tan, Jun Jie

    2017-06-01

    Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34 + hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) generation in vitro. This study aimed to investigate the effects of MSCs on HSC expansion and differentiation. HSCs were co-cultured with MSCs or with 10% MSCs-derived conditioned medium, with HSCs cultured under standard medium served as a control. Cell expansion rates, number of mononuclear cell post-expansion and number of enucleated cells post-differentiation were evaluated. HSCs showed superior proliferation in the presence of MSC with mean expansion rate of 3.5 × 10 8  ± 1.8 × 10 7 after day 7 compared to the conditioned medium and the control group (8.9 × 10 7  ± 1.1 × 10 8 and 7.0 × 10 7  ± 3.3 × 10 6 respectively, P cell was greater compared to earlier passages, indicating successful RBC differentiation. Cord blood-derived CD34 + HSCs can be greatly expanded by co-culturing with MSCs without affecting the RBC differentiation capability, suggesting the importance of direct MSC-HSCs contact in HSC expansion and RBC differentiation. © 2017 International Federation for Cell Biology.

  10. Complete Genome Sequence of a Human-Invasive Salmonella enterica Serovar Typhimurium Strain of the Emerging Sequence Type 213 Harboring a Multidrug Resistance IncA/C Plasmid and a blaCMY-2-Carrying IncF Plasmid.

    Science.gov (United States)

    Silva, Claudia; Calva, Edmundo; Calva, Juan J; Wiesner, Magdalena; Fernández-Mora, Marcos; Puente, José L; Vinuesa, Pablo

    2015-11-12

    Salmonella enterica subsp. enterica serovar Typhimurium strain 33676 was isolated in Mexico City, Mexico, from a patient with a systemic infection, and its complete genome sequence was determined using PacBio single-molecule real-time technology. Strain 33676 harbors an IncF plasmid carrying the extended-spectrum cephalosporin gene blaCMY-2 and a multidrug resistance IncA/C plasmid. Copyright © 2015 Silva et al.

  11. Exerting Capacity.

    Science.gov (United States)

    Leger, J Michael; Phillips, Carolyn A

    2017-05-01

    Patient safety has been at the forefront of nursing research since the release of the Institute of Medicine's report estimating the number of preventable adverse events in hospital settings; yet no research to date has incorporated the perspectives of bedside nurses using classical grounded theory (CGT) methodology. This CGT study explored the perceptions of bedside registered nurses regarding patient safety in adult acute care hospitals. Data analysis used three techniques unique to CGT-the constant comparative method, coding, and memoing-to explore the values, realities, and beliefs of bedside nurses about patient safety. The analysis resulted in a substantive theory, Exerting Capacity, which explained how bedside nurses balance the demands of keeping their patients safe. Exerting Capacity has implications for health care organization leaders, nursing leaders, and bedside nurses; it also has indications for future research into the concept of patient safety.

  12. Sodium phenylbutyrate, a drug with known capacity to reduce endoplasmic reticulum stress, partially alleviates lipid-induced insulin resistance and beta-cell dysfunction in humans.

    Science.gov (United States)

    Xiao, Changting; Giacca, Adria; Lewis, Gary F

    2011-03-01

    Chronically elevated free fatty acids contribute to insulin resistance and pancreatic β-cell failure. Among numerous potential factors, the involvement of endoplasmic reticulum (ER) stress has been postulated to play a mechanistic role. Here we examined the efficacy of the chemical chaperone, sodium phenylbutyrate (PBA), a drug with known capacity to reduce ER stress in animal models and in vitro, on lipid-induced insulin resistance and β-cell dysfunction in humans. Eight overweight or obese nondiabetic men underwent four studies each, in random order, 4 to 6 weeks apart. Two studies were preceded by 2 weeks of oral PBA (7.5 g/day), followed by a 48-h i.v. infusion of intralipid/heparin or saline, and two studies were preceded by placebo treatment, followed by similar infusions. Insulin secretion rates (ISRs) and sensitivity (S(I)) were assessed after the 48-h infusions by hyperglycemic and hyperinsulinemic-euglycemic clamps, respectively. Lipid infusion reduced S(I), which was significantly ameliorated by pretreatment with PBA. Absolute ISR was not affected by any treatment; however, PBA partially ameliorated the lipid-induced reduction in the disposition index (DI = ISR × S(I)), indicating that PBA prevented lipid-induced β-cell dysfunction. These results suggest that PBA may provide benefits in humans by ameliorating the insulin resistance and β-cell dysfunction induced by prolonged elevation of free fatty acids.

  13. Characterization of the cloned full-length and a truncated human target of rapamycin: Activity, specificity, and enzyme inhibition as studied by a high capacity assay

    International Nuclear Information System (INIS)

    Toral-Barza, Lourdes; Zhang Weiguo; Lamison, Craig; LaRocque, James; Gibbons, James; Yu, Ker

    2005-01-01

    The mammalian target of rapamycin (mTOR/TOR) is implicated in cancer and other human disorders and thus an important target for therapeutic intervention. To study human TOR in vitro, we have produced in large scale both the full-length TOR (289 kDa) and a truncated TOR (132 kDa) from HEK293 cells. Both enzymes demonstrated a robust and specific catalytic activity towards the physiological substrate proteins, p70 S6 ribosomal protein kinase 1 (p70S6K1) and eIF4E binding protein 1 (4EBP1), as measured by phosphor-specific antibodies in Western blotting. We developed a high capacity dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA) for analysis of kinetic parameters. The Michaelis constant (K m ) values of TOR for ATP and the His6-S6K substrate were shown to be 50 and 0.8 μM, respectively. Dose-response and inhibition mechanisms of several known inhibitors, the rapamycin-FKBP12 complex, wortmannin and LY294002, were also studied in DELFIA. Our data indicate that TOR exhibits kinetic features of those shared by traditional serine/threonine kinases and demonstrate the feasibility for TOR enzyme screen in searching for new inhibitors

  14. Decreased cell survival and DNA repair capacity after UVC irradiation in association with down-regulation of GRP78/BiP in human RSa cells

    International Nuclear Information System (INIS)

    Zhai Ling; Kita, Kazuko; Wano, Chieko; Wu Yuping; Sugaya, Shigeru; Suzuki, Nobuo

    2005-01-01

    In contrast to extensive studies on the roles of molecular chaperones, such as heat shock proteins, there are only a few reports about the roles of GRP78/BiP, an endoplasmic reticulum (ER) stress-induced molecular chaperone, in mammalian cell responses to DNA-damaging stresses. To investigate whether GRP78/BiP is involved in resistance to a DNA-damaging agent, UVC (principally 254 nm in wavelength), we established human cells with down-regulation of GRP78/BiP by transfection of human RSa cells with antisense cDNA for GRP78/BiP. We found that the transfected cells showed higher sensitivity to UVC-induced cell death than control cells transfected with the vector alone. In the antisense-cDNA transfected cells, the removal capacities of the two major types of UVC-damaged DNA (thymine dimers and (6-4) photoproducts) in vivo and DNA synthesis activity of whole cell extracts to repair UVC-irradiated plasmids in vitro were remarkably decreased compared with those in the control cells. Furthermore, the antisense-cDNA transfected cells also showed slightly higher sensitivity to cisplatin-induced cell death than the control cells. Cisplatin-induced DNA damage is primarily repaired by nucleotide excision repair, like UVC-induced DNA damage. The present results suggest that GRP78/BiP plays a protective role against UVC-induced cell death possibly via nucleotide excision repair, at least in the human RSa cells tested

  15. The Human Capital of Knowledge Brokers: An analysis of attributes, capacities and skills of academic teaching and research faculty at Kenyan schools of public health.

    Science.gov (United States)

    Jessani, Nasreen; Kennedy, Caitlin; Bennett, Sara

    2016-08-02

    enhance their collective human capital and influence on public health policy and practice. Capacity strengthening of tangible skills and recognition of less tangible personality characteristics could contribute to enhanced academic-policymaker networks. These, in turn, could contribute to the relevance of SPH research and teaching programs as well as evidence-informed public health policies.

  16. Adhesion profile and differentiation capacity of human adipose tissue derived mesenchymal stem cells grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite nano-coated surfaces.

    Science.gov (United States)

    Bostancioglu, R Beklem; Gurbuz, Mevlut; Akyurekli, Ayse Gul; Dogan, Aydin; Koparal, A Savas; Koparal, A Tansu

    2017-07-01

    Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human adipose tissue derived mesenchymal stem cells (hMSC)', were grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite (HAP) nano-coated surfaces. These metal ions are known to have different chemical and surface properties; therefore we investigated their respective contributions to cell viability, cellular behavior, osteogenic differentiation capacity and substrate-cell interaction. Nano-powders were produced using a wet chemical process. Air spray deposition was used to accumulate the metal ion doped HAP films on a glass substrate. Cell viability was determined by MTT, LDH and DNA quantitation methods Osteogenic differentiation capacity of hMSCs was analyzed with Alizarin Red Staining and Alkaline Phosphatase Specific Activity. Adhesion of the hMSCs and the effect of cell adhesion on biomaterial biocompatibility were explored through cell adhesion assay, immunofluorescence staining for vinculin and f-actin cytoskeleton components, SEM and microarray including 84 known extracellular matrix proteins and cell adhesion pathway genes, since, adhesion is the first step for good biocompability. The results demonstrate that the viability and osteogenic differentiation of the hMSCs (in growth media without osteogenic stimulation) and cell adhesion capability are higher on nanocoated surfaces that include Zn, Ag and/or Cu metal ions than commercial HAP. These results reveal that Zn, Ag and Cu metal ions contribute to the biocompatibility of exogenous material. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage

    Directory of Open Access Journals (Sweden)

    MM Pleumeekers

    2014-04-01

    Full Text Available Cartilage has limited self-regenerative capacity. Tissue engineering can offer promising solutions for reconstruction of missing or damaged cartilage. A major challenge herein is to define an appropriate cell source that is capable of generating a stable and functional matrix. This study evaluated the performance of culture-expanded human chondrocytes from ear (EC, nose (NC and articular joint (AC, as well as bone-marrow-derived and adipose-tissue-derived mesenchymal stem cells both in vitro and in vivo. All cells (≥ 3 donors per source were culture-expanded, encapsulated in alginate and cultured for 5 weeks. Subsequently, constructs were implanted subcutaneously for 8 additional weeks. Before and after implantation, glycosaminoglycan (GAG and collagen content were measured using biochemical assays. Mechanical properties were determined using stress-strain-indentation tests. Hypertrophic differentiation was evaluated with qRT-PCR and subsequent endochondral ossification with histology. ACs had higher chondrogenic potential in vitro than the other cell sources, as assessed by gene expression and GAG content (p < 0.001. However, after implantation, ACs did not further increase their matrix. In contrast, ECs and NCs continued producing matrix in vivo leading to higher GAG content (p < 0.001 and elastic modulus. For NC-constructs, matrix-deposition was associated with the elastic modulus (R2 = 0.477, p = 0.039. Although all cells – except ACs – expressed markers for hypertrophic differentiation in vitro, there was no bone formed in vivo. Our work shows that cartilage formation and functionality depends on the cell source used. ACs possess the highest chondrogenic capacity in vitro, while ECs and NCs are most potent in vivo, making them attractive cell sources for cartilage repair.

  18. Reduction in cardiolipin decreases mitochondrial spare respiratory capacity and increases glucose transport into and across human brain cerebral microvascular endothelial cells.

    Science.gov (United States)

    Nguyen, Hieu M; Mejia, Edgard M; Chang, Wenguang; Wang, Ying; Watson, Emily; On, Ngoc; Miller, Donald W; Hatch, Grant M

    2016-10-01

    Microvessel endothelial cells form part of the blood-brain barrier, a restrictively permeable interface that allows transport of only specific compounds into the brain. Cardiolipin is a mitochondrial phospholipid required for function of the electron transport chain and ATP generation. We examined the role of cardiolipin in maintaining mitochondrial function necessary to support barrier properties of brain microvessel endothelial cells. Knockdown of the terminal enzyme of cardiolipin synthesis, cardiolipin synthase, in hCMEC/D3 cells resulted in decreased cellular cardiolipin levels compared to controls. The reduction in cardiolipin resulted in decreased mitochondrial spare respiratory capacity, increased pyruvate kinase activity, and increased 2-deoxy-[(3) H]glucose uptake and glucose transporter-1 expression and localization to membranes in hCMEC/D3 cells compared to controls. The mechanism for the increase in glucose uptake was an increase in adenosine-5'-monophosphate kinase and protein kinase B activity and decreased glycogen synthase kinase 3 beta activity. Knockdown of cardiolipin synthase did not affect permeability of fluorescent dextran across confluent hCMEC/D3 monolayers grown on Transwell(®) inserts. In contrast, knockdown of cardiolipin synthase resulted in an increase in 2-deoxy-[(3) H]glucose transport across these monolayers compared to controls. The data indicate that in hCMEC/D3 cells, spare respiratory capacity is dependent on cardiolipin. In addition, reduction in cardiolipin in these cells alters their cellular energy status and this results in increased glucose transport into and across hCMEC/D3 monolayers. Microvessel endothelial cells form part of the blood-brain barrier, a restrictively permeable interface that allows transport of only specific compounds into the brain. In human adult brain endothelial cell hCMEC/D3 monolayers cultured on Transwell(®) plates, knockdown of cardiolipin synthase results in decrease in mitochondrial

  19. Cytotoxic capacity of IL-15-stimulated cytokine-induced killer cells against human acute myeloid leukemia and rhabdomyosarcoma in humanized preclinical mouse models

    Directory of Open Access Journals (Sweden)

    Eva eRettinger

    2012-04-01

    Full Text Available Allogeneic stem cell transplantation (allo-SCT has become an important treatment modality for patients with high risk acute myeloid leukemia (AML and is also under investigation for soft tissue sarcomas. The therapeutic success is still limited by minimal residual disease (MRD status ultimately leading to patients’ relapse. Adoptive donor lymphocyte infusions (DLI based on MRD status using IL-15-expanded cytokine-induced killer (CIK cells may prevent relapse without causing graft-versus-host-disease (GvHD. To generate preclinical data we developed mouse models to study anti-leukemic- and anti-tumor-potential of CIK cells in vivo. Immunodeficient mice (NOD/SCID/IL2Rγc-, NSG were injected intravenously with human leukemic cell lines THP-1, SH-2 and with human rhabdomyosarcoma (RMS cell lines RH41 and RH30 at minimal doses required for leukemia or tumor engraftment. Mice transplanted with THP-1 or RH41 cells were randomly assigned for analysis of CIK cell treatment. Organs of mice were analyzed by flow cytometry as well as quantitative polymerase chain reaction (qPCR for engraftment of malignant cells and CIK cells. Potential of CIK cells to induce GvHD was determined by histological analysis. Tissues of the highest degree of THP-1 cell expansion included bone marrow (BM followed by liver, lung, spleen, peripheral blood (PB, and brain. RH30 and RH41 engraftment mainly took place in liver and lung, but was also detectable in spleen and PB. In spite of delayed CIK cell expansion compared with malignant cells, CIK cells injected at an effector to target cell (E:T ratio of 1:1 were sufficient for significant reduction of RH41 cells, whereas against fast-expanding THP-1 cells an E:T ratio of 250:1 was needed to achieve comparable results. Our preclinical in vivo mouse models showed a reliably 100% engraftment of malignant cells which is essential for analysis of anti-cancer therapy. Furthermore our data demonstrated that IL-15-activated CIK cells

  20. Capacity Building

    International Nuclear Information System (INIS)

    Molloy, Brian; Mallick, Shahid

    2014-01-01

    Outcomes & Recommendations: • Significant increase needed in the nuclear workforce both to replace soon-to-retire current generation and to staff large numbers of new units planned • Key message, was the importance of an integrated approach to workforce development. • IAEA and other International Organisations were asked to continue to work on Knowledge Management, Networks and E&T activities • IAEA requested to conduct Global Survey of HR needs – survey initiated but only 50% of operating countries (30% of capacity) took part, so results inconclusive