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Sample records for human afs cell

  1. Clone-derived human AF-amniotic fluid stem cells are capable of skeletal myogenic differentiation in vitro and in vivo.

    Science.gov (United States)

    Ma, Xiaorong; Zhang, Shengli; Zhou, Junmei; Chen, Baisong; Shang, Yafeng; Gao, Tongbing; Wang, Xue; Xie, Hua; Chen, Fang

    2012-08-01

    Stem cell-based therapy may be the most promising method to cure skeletal muscle degenerative diseases such as Duchenne muscular dystrophy (DMD) and trauma in the future. Human amniotic fluid is enriched with early-stage stem cells from developing fetuses and these cells have cardiomyogenic potential both in vitro and in vivo. In the present study, we investigated the characteristics of human amniotic fluid-derived AF-type stem (HAF-AFS) cells by flow cytometry, immunofluorescence staining, reverse-transcription polymerase chain reaction, and osteogenic and adipogenic differentiation analysis. After confirming the stemness of HAF-AFS cells, we tested whether HAF-AFS cells could differentiate into skeletal myogenic cells in vitro and incorporate into regenerating skeletal muscle in vivo. By temporary exposure to the DNA demethylation agent 5-aza-2'-deoxycytidine (5-Aza dC) or co-cultured with C2C12 myoblasts, HAF-AFS cells differentiated into skeletal myogenic cells, expressing skeletal myogenic cell-specific markers such as Desmin, Troponin I (Tn I) and α-Actinin. Four weeks after transplantation into cardiotoxin-injured and X-ray-irradiated tibialis anterior (TA) muscles of NOD/SCID mice, HAF-AFS cells survived, differentiated into myogenic precursor cells and fused with host myofibres. The findings that HAF-AFS cells differentiate into myogenic cells in vitro and incorporate in skeletal muscle regeneration in vivo hold the promise of HAF-AFS cell-based therapy for skeletal muscle degenerative diseases.

  2. Patentering af det humane genom

    DEFF Research Database (Denmark)

    Sommer, Tine

    2004-01-01

    Direktiv 98/44/EF om retlig beskyttelse af bioteknologiske opfindelser blev gennemført i dansk ret med ikrafttrædelse den 30. juli 2000. Direktivet indeholder i artikel 5 en central bestemmelse som giver adgang til patent på humane gener. I artikel 5, stk. 3, er indføjet et skærpet krav til...

  3. MLL-AF9-mediated immortalization of human hematopoietic cells along different lineages changes during ontogeny

    NARCIS (Netherlands)

    Horton, S J; Jaques, J; Woolthuis, C; van Dijk, J; Mesuraca, M; Huls, G; Morrone, G; Vellenga, E; Schuringa, J J

    The MLL-AF9 fusion gene is associated with aggressive leukemias of both the myeloid and lymphoid lineage in infants, whereas in adults, this translocation is mainly associated with acute myeloid leukemia. These observations suggest that differences exist between fetal and adult tissues in terms of

  4. MLL-AF9-mediated immortalization of human hematopoietic cells along different lineages changes during ontogeny

    NARCIS (Netherlands)

    Horton, S J; Jaques, J; Woolthuis, C; van Dijk, J; Mesuraca, M; Huls, G; Morrone, G; Vellenga, E; Schuringa, J J

    2013-01-01

    The MLL-AF9 fusion gene is associated with aggressive leukemias of both the myeloid and lymphoid lineage in infants, whereas in adults, this translocation is mainly associated with acute myeloid leukemia. These observations suggest that differences exist between fetal and adult tissues in terms of t

  5. Forebyggelse af vertikal transmission af human immundefektvirus i Danmark

    DEFF Research Database (Denmark)

    Rasmussen, Maria Birkvad; Rasmussen, Johannes Boyen; Nielsen, Vibeke Rosenfeldt

    2008-01-01

    INTRODUCTION: Human immunodeficiency virus (HIV) is a RNA virus that can be transmitted parenterally, sexually or vertically. An effective prevention strategy has been implemented in industrialised countries, thereby reducing vertical transmission from 15-25% to

  6. AF10 plays a key role in the survival of uncommitted hematopoietic cells.

    Directory of Open Access Journals (Sweden)

    Raquel Chamorro-Garcia

    Full Text Available Hematopoiesis is a complex process regulated by both cell intrinsic and cell extrinsic factors. Alterations in the expression of critical genes during hematopoiesis can modify the balance between stem cell differentiation and proliferation, and may ultimately give rise to leukemia and other diseases. AF10 is a transcription factor that has been implicated in the development of leukemia following chromosomal rearrangements between the AF10 gene and one of at least two other genes, MLL and CALM. The link between AF10 and leukemia, together with the known interactions between AF10 and hematopoietic regulators, suggests that AF10 may be important in hematopoiesis and in leukemic transformation. Here we show that AF10 is important for proper hematopoietic differentiation. The induction of hematopoietic differentiation in both human hematopoietic cell lines and murine total bone marrow cells triggers a decrease of AF10 mRNA and protein levels, particularly in stem cells and multipotent progenitors. Gain- and loss-of-function studies demonstrate that over- or under-expression of AF10 leads to apoptotic cell death in stem cells and multipotent progenitors. We conclude that AF10 plays a key role in the maintenance of multipotent hematopoietic cells.

  7. Forebyggelse af vertikal transmission af human immundefektvirus i Danmark

    DEFF Research Database (Denmark)

    Rasmussen, Maria Birkvad; Rasmussen, Johannes Boyen; Nielsen, Vibeke Rosenfeldt

    2008-01-01

    INTRODUCTION: Human immunodeficiency virus (HIV) is a RNA virus that can be transmitted parenterally, sexually or vertically. An effective prevention strategy has been implemented in industrialised countries, thereby reducing vertical transmission from 15-25% to ... was not initiated. CONCLUSION: As long as preventive treatment strategies are followed, there is no transmission of HIV from mother to child, neither during pregnancy nor during or after birth....... was to describe vertical transmission of HIV in Denmark after the introduction of ART. MATERIALS AND METHODS: The study was a retrospective study of all HIV-infected women who gave birth in Denmark between 1 January 2000 and 31 May 2005 and their children. RESULTS: 83 HIV-infected women gave birth to 96 children...

  8. The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells.

    Science.gov (United States)

    Whisenant, Thomas C; Peralta, Eigen R; Aarreberg, Lauren D; Gao, Nina J; Head, Steven R; Ordoukhanian, Phillip; Williamson, Jamie R; Salomon, Daniel R

    2015-01-01

    Activation of CD4 T cells is a reaction to challenges such as microbial pathogens, cancer and toxins that defines adaptive immune responses. The roles of T cell receptor crosslinking, intracellular signaling, and transcription factor activation are well described, but the importance of post-transcriptional regulation by RNA-binding proteins (RBPs) has not been considered in depth. We describe a new model expanding and activating primary human CD4 T cells and applied this to characterizing activation-induced assembly of splicing factors centered on U2AF2. We immunoprecipitated U2AF2 to identify what mRNA transcripts were bound as a function of activation by TCR crosslinking and costimulation. In parallel, mass spectrometry revealed the proteins incorporated into the U2AF2-centered RNA/protein interactome. Molecules that retained interaction with the U2AF2 complex after RNAse treatment were designated as "central" interactome members (CIMs). Mass spectrometry also identified a second class of activation-induced proteins, "peripheral" interactome members (PIMs), that bound to the same transcripts but were not in physical association with U2AF2 or its partners. siRNA knockdown of two CIMs and two PIMs caused changes in activation marker expression, cytokine secretion, and gene expression that were unique to each protein and mapped to pathways associated with key aspects of T cell activation. While knocking down the PIM, SYNCRIP, impacts a limited but immunologically important set of U2AF2-bound transcripts, knockdown of U2AF1 significantly impairs assembly of the majority of protein and mRNA components in the activation-induced interactome. These results demonstrated that CIMs and PIMs, either directly or indirectly through RNA, assembled into activation-induced U2AF2 complexes and play roles in post-transcriptional regulation of genes related to cytokine secretion. These data suggest an additional layer of regulation mediated by the activation-induced assembly of RNA

  9. Structure and biological activities of eumenine mastoparan-AF (EMP-AF), a new mast cell degranulating peptide in the venom of the solitary wasp (Anterhynchium flavomarginatum micado).

    Science.gov (United States)

    Konno, K; Hisada, M; Naoki, H; Itagaki, Y; Kawai, N; Miwa, A; Yasuhara, T; Morimoto, Y; Nakata, Y

    2000-11-01

    A new mast cell degranulating peptide, eumenine mastoparan-AF (EMP-AF), was isolated from the venom of the solitary wasp Anterhynchium flavomarginatum micado, the most common eumenine wasp found in Japan. The structure was analyzed by FAB-MS/MS together with Edman degradation, which was corroborated by solid-phase synthesis. The sequence of EMP-AF, Ile-Asn-Leu-Leu-Lys-Ile-Ala-Lys-Gly-Ile-Ile-Lys-Ser-Leu-NH(2), was similar to that of mastoparan, a mast cell degranulating peptide from a hornet venom; tetradecapeptide with C-terminus amidated and rich in hydrophobic and basic amino acids. In fact, EMP-AF exhibited similar activity to mastoparan in stimulating degranulation from rat peritoneal mast cells and RBL-2H3 cells. It also showed significant hemolytic activity in human erythrocytes. Therefore, this is the first example that a mast cell degranulating peptide is found in the solitary wasp venom. Besides the degranulation and hemolytic activity, EMP-AF also affects on neuromuscular transmission in the lobster walking leg preparation. Three analogs EMP-AF-1 approximately 3 were snythesized and biologically tested together with EMP-AF, resulting in the importance of the C-terminal amide structure for biological activities.

  10. A human ESC model for MLL-AF4 leukemic fusion gene reveals an impaired early hematopoietic-endothelial specification

    Institute of Scientific and Technical Information of China (English)

    Clara Bueno; Agustin F Femández; Mario F Fraga; Inmaculada Moreno-Gimeno; Deborah Burks; Maria del Carmen Plaza-Calonge; Juan C Rodríguez-Manzaneque; Pablo Menendez; Rosa Montes; Gustavo J Melen; Verónica Ramos-Mejia; Pedro J Real; Verónica Ayllón; Laura Sanchez; Gertrudis Ligero; Iván Gutierrez-Aranda

    2012-01-01

    The MLL-AF4 fusion gene is a hallmark genomic aberration in high-risk acute lymphoblastic leukemia in inants.Although it is well established that MLL-AF4 arises prenatally during human development,its effects on hematopoieric development in utero remain unexplored.We have created a human-specific cellular system to study early hemato-endothelial development in MLL-AF4-expressing human embryonic stem cells (hESCs).Functional studies,clonal analysis and gene expression profiling reveal that expression of MLL-AF4 in hESCs has a phenotypic,functional and gene expression impact.MLL-AF4 acts as a global transcriptional activator and a positive regulator of homeobox gene expression in hESCs.Functionally,MLL-AF4 enhances the specification of hemogenic precursors from hESCs but strongly impairs further hematopoietic commitment in favor of an endothelial cell fate.MLL-AF4 hESCs are transcriptionally primed to differentiate towards hemogenic precursors prone to endothelial maturation,as reflected by the marked upregulation of master genes associated to vascular-endothelial functions and early hematopoiesis.Furthermore,we report that MLL-AF4 expression is not sufficient to transform hESC-derived hematopoietic cells.This work illustrates how hESCs may provide unique insights into human development and further our understanding of how leukemic fusion genes,known to arise prenatally,regulate human embryonic hematopoietic specification.

  11. Mylar and Teflon-AF as cell culture substrates for studying endothelial cell adhesion.

    Science.gov (United States)

    Anamelechi, Charles C; Truskey, George A; Reichert, W Monty

    2005-12-01

    The textured and opaque nature of Dacron and ePTFE has prevented the use of these fabrics in conventional cell culture techniques normally employed to optimize cell attachment and retention. This lack of optimization has led, in part, to the poor performance of endothelialization strategies for improving vascular graft patency. Here we show that thin, transparent films of Mylar and Teflon-AF are viable in vitro cell culture mimics of Dacron and ePTFE vascular graft materials, particularly for the study of protein mediated endothelial cell (EC) attachment, spreading and adhesion. Glass substrates were used as controls. X-ray photoelectron spectroscopy (XPS) and contact angle analysis showed that Mylar and Teflon-AF have surface chemistries that closely match Dacron and ePTFE. (125)I radiolabeling was used to quantify fibronectin (FN) adsorption, and FN and biotinylated-BSA "dual ligand" co-adsorption onto glass, Mylar and Teflon-AF substrates. Native human umbilical vein endothelial cells (HUVEC) and streptavidin-incubated biotinylated-HUVEC (SA-b-HUVEC) spreading was measured using phase contrast microscopy. Cell retention and adhesion was determined using phase contrast microscopy under laminar flow. All surfaces lacking protein pre-treatment, regardless of surface type, showed the lowest degree of cell spreading and retention. Dual ligand treated Mylar films showed significantly greater SA-b-HUVEC spreading up to 2 h, but were similar to HUVEC on FN treated Mylar at longer times; whereas SA-b-HUVEC spreading on dual ligand treated Teflon-AF was never significantly different from HUVEC on FN treated Teflon-AF at any time point. SA-b-HUVEC retention was significantly greater on dual ligand treated Mylar compared to HUVEC on FN treated Mylar over the entire range of shear stresses tested (3.54-28.3 dynes/cm(2)); whereas SA-b-HUVEC retention to dual ligand and HUVEC retention to FN treated Teflon-AF gave similar results at each shear stress, with only the mid

  12. Heat shock proteiners inhibering af programmeret celledød

    OpenAIRE

    Cohen, Malene; Jantzen, Kim; Olsson, Kim; Rohde, Anders

    2007-01-01

    Heat shock proteiner (Hsp) har i de seneste år været genstand for interesse indenfor forskellige grene af biologien på grund af deres egenskaber indenfor programmeret celledød. Den etablerede viden er størst indenfor oncologi, hvor Hsps apoptosehæmmende egenskaber fortsat er genstand for forskning, men proteinernes præventive effekt på toksin- eller stressinduceret celledød har spredt interessen til felter som neurologi og toksologi. Målet med denne rapport har været at sammenf...

  13. Fuel Cell Shaft Power Pack - Regulering af brændselsceller

    DEFF Research Database (Denmark)

    Andreasen, Søren Juhl

    2009-01-01

    Afsluttende formidling af forskningsresultater i forbindelse med projektet Fuel Cell Shaft Power Pack......Afsluttende formidling af forskningsresultater i forbindelse med projektet Fuel Cell Shaft Power Pack...

  14. File list: His.Bld.05.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Bld.05.AllAg.MLL-AF9_leukemic_cell mm9 Histone Blood MLL-AF9 leukemic cell SRX8...,SRX692065,SRX692070,SRX692075,SRX692080,SRX734388,SRX884081 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Bld.05.AllAg.MLL-AF9_leukemic_cell.bed ...

  15. File list: InP.Bld.10.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Bld.10.AllAg.MLL-AF10_leukemic_cell mm9 Input control Blood MLL-AF10 leukemic c...ell SRX671749 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Bld.10.AllAg.MLL-AF10_leukemic_cell.bed ...

  16. File list: NoD.Bld.50.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Bld.50.AllAg.MLL-AF10_leukemic_cell mm9 No description Blood MLL-AF10 leukemic ...cell http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.Bld.50.AllAg.MLL-AF10_leukemic_cell.bed ...

  17. File list: His.Bld.50.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Bld.50.AllAg.MLL-AF10_leukemic_cell mm9 Histone Blood MLL-AF10 leukemic cell SR...SRX671755,SRX671765,SRX671754 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Bld.50.AllAg.MLL-AF10_leukemic_cell.bed ...

  18. File list: His.Bld.20.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Bld.20.AllAg.MLL-AF10_leukemic_cell mm9 Histone Blood MLL-AF10 leukemic cell SR...SRX671761,SRX671759,SRX671756 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Bld.20.AllAg.MLL-AF10_leukemic_cell.bed ...

  19. File list: Oth.Bld.50.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Bld.50.AllAg.MLL-AF10_leukemic_cell mm9 TFs and others Blood MLL-AF10 leukemic ...cell http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Bld.50.AllAg.MLL-AF10_leukemic_cell.bed ...

  20. File list: DNS.Bld.50.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Bld.50.AllAg.MLL-AF9_leukemic_cell mm9 DNase-seq Blood MLL-AF9 leukemic cell ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.Bld.50.AllAg.MLL-AF9_leukemic_cell.bed ...

  1. File list: NoD.Bld.20.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Bld.20.AllAg.MLL-AF9_leukemic_cell mm9 No description Blood MLL-AF9 leukemic ce...ll http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.Bld.20.AllAg.MLL-AF9_leukemic_cell.bed ...

  2. File list: NoD.Bld.05.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Bld.05.AllAg.MLL-AF9_leukemic_cell mm9 No description Blood MLL-AF9 leukemic ce...ll http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.Bld.05.AllAg.MLL-AF9_leukemic_cell.bed ...

  3. File list: His.Bld.10.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Bld.10.AllAg.MLL-AF10_leukemic_cell mm9 Histone Blood MLL-AF10 leukemic cell SR...SRX671756,SRX671754,SRX671761 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Bld.10.AllAg.MLL-AF10_leukemic_cell.bed ...

  4. File list: DNS.Bld.20.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Bld.20.AllAg.MLL-AF10_leukemic_cell mm9 DNase-seq Blood MLL-AF10 leukemic cell ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.Bld.20.AllAg.MLL-AF10_leukemic_cell.bed ...

  5. File list: His.Bld.50.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Bld.50.AllAg.MLL-AF9_leukemic_cell mm9 Histone Blood MLL-AF9 leukemic cell SRX8...,SRX115551,SRX692071,SRX734388,SRX692061,SRX061979,SRX884081 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Bld.50.AllAg.MLL-AF9_leukemic_cell.bed ...

  6. File list: NoD.Bld.10.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Bld.10.AllAg.MLL-AF9_leukemic_cell mm9 No description Blood MLL-AF9 leukemic ce...ll http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.Bld.10.AllAg.MLL-AF9_leukemic_cell.bed ...

  7. File list: Unc.Bld.50.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Bld.50.AllAg.MLL-AF9_leukemic_cell mm9 Unclassified Blood MLL-AF9 leukemic cell... SRX692060,SRX692059 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.Bld.50.AllAg.MLL-AF9_leukemic_cell.bed ...

  8. File list: His.Bld.05.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Bld.05.AllAg.MLL-AF10_leukemic_cell mm9 Histone Blood MLL-AF10 leukemic cell SR...SRX671759,SRX671756,SRX671755 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Bld.05.AllAg.MLL-AF10_leukemic_cell.bed ...

  9. File list: DNS.Bld.50.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Bld.50.AllAg.MLL-AF10_leukemic_cell mm9 DNase-seq Blood MLL-AF10 leukemic cell ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.Bld.50.AllAg.MLL-AF10_leukemic_cell.bed ...

  10. File list: Unc.Bld.20.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Bld.20.AllAg.MLL-AF9_leukemic_cell mm9 Unclassified Blood MLL-AF9 leukemic cell... SRX692060,SRX692059 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.Bld.20.AllAg.MLL-AF9_leukemic_cell.bed ...

  11. File list: InP.Bld.20.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Bld.20.AllAg.MLL-AF10_leukemic_cell mm9 Input control Blood MLL-AF10 leukemic c...ell SRX671749 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Bld.20.AllAg.MLL-AF10_leukemic_cell.bed ...

  12. File list: Pol.Bld.50.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bld.50.AllAg.MLL-AF10_leukemic_cell mm9 RNA polymerase Blood MLL-AF10 leukemic ...cell SRX671766,SRX671757 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Bld.50.AllAg.MLL-AF10_leukemic_cell.bed ...

  13. File list: Unc.Bld.50.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Bld.50.AllAg.MLL-AF10_leukemic_cell mm9 Unclassified Blood MLL-AF10 leukemic ce...ll SRX671758 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.Bld.50.AllAg.MLL-AF10_leukemic_cell.bed ...

  14. File list: DNS.Bld.05.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Bld.05.AllAg.MLL-AF9_leukemic_cell mm9 DNase-seq Blood MLL-AF9 leukemic cell ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.Bld.05.AllAg.MLL-AF9_leukemic_cell.bed ...

  15. File list: InP.Bld.05.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Bld.05.AllAg.MLL-AF10_leukemic_cell mm9 Input control Blood MLL-AF10 leukemic c...ell SRX671749 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Bld.05.AllAg.MLL-AF10_leukemic_cell.bed ...

  16. File list: NoD.Bld.10.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Bld.10.AllAg.MLL-AF10_leukemic_cell mm9 No description Blood MLL-AF10 leukemic ...cell http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.Bld.10.AllAg.MLL-AF10_leukemic_cell.bed ...

  17. File list: NoD.Bld.05.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Bld.05.AllAg.MLL-AF10_leukemic_cell mm9 No description Blood MLL-AF10 leukemic ...cell http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.Bld.05.AllAg.MLL-AF10_leukemic_cell.bed ...

  18. File list: Unc.Bld.10.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Bld.10.AllAg.MLL-AF10_leukemic_cell mm9 Unclassified Blood MLL-AF10 leukemic ce...ll SRX671758 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.Bld.10.AllAg.MLL-AF10_leukemic_cell.bed ...

  19. File list: Pol.Bld.10.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Bld.10.AllAg.MLL-AF10_leukemic_cell mm9 RNA polymerase Blood MLL-AF10 leukemic ...cell SRX671766,SRX671757 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Bld.10.AllAg.MLL-AF10_leukemic_cell.bed ...

  20. File list: Oth.Bld.05.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Bld.05.AllAg.MLL-AF10_leukemic_cell mm9 TFs and others Blood MLL-AF10 leukemic ...cell http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Bld.05.AllAg.MLL-AF10_leukemic_cell.bed ...

  1. File list: Oth.ALL.10.Pou2af1.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.10.Pou2af1.AllCell mm9 TFs and others Pou2af1 All cell types SRX877593,SRX8...77596 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.10.Pou2af1.AllCell.bed ...

  2. File list: Oth.ALL.05.Pou2af1.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.05.Pou2af1.AllCell mm9 TFs and others Pou2af1 All cell types SRX877593,SRX8...77596 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.05.Pou2af1.AllCell.bed ...

  3. File list: Oth.ALL.20.Pou2af1.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.20.Pou2af1.AllCell mm9 TFs and others Pou2af1 All cell types SRX877596,SRX8...77593 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.20.Pou2af1.AllCell.bed ...

  4. File list: InP.Bld.05.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  5. File list: InP.Bld.50.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  6. Acquired resistance to auranofin in cultured human cells.

    Science.gov (United States)

    Glennås, A; Rugstad, H E

    1985-01-01

    A substrain (HEAF) of cultured human epithelial cells, grown as monolayers, was selected for resistance to auranofin (AF), a gold-containing anti-arthritic drug, by growing the parental HE cells with stepwise increased concentrations of AF in the medium. HEAF cells acquired resistance to 2 mumol AF/l, twice the concentration tolerated by the sensitive HE cells. Resistance to AF was also demonstrated in another substrain (HE100) originally selected for by its cadmium resistance, and characterized by a high cytosolic metallothionein (MT) content. Following continuous exposure to 2 mumol AF/l for 4 days, 58% of the HEAF cells, 67% of the HE100 cells, and 16% of the HE cells remained adherent to the flasks, compared with non-treated controls. Following 24 h AF exposure to living cells, HEAF cells had one-half and HE100 cells twice the cellular and cytosolic gold concentration per mg protein, as compared with HE cells. Gel filtration of cell cytosols revealed gold-binding proteins with a mol. wt. of about 10 000 apparently occurring on AF exposure in HEAF and HE cells. They bound 10-15% of cytosolic gold. MT in HE100 cells bound AF-gold to about the same extent. We suggest that the ability of cells to maintain the gold concentration at a low level (HEAF) and trapping of gold by MT (HE100) or low molecular weight proteins occurring on AF treatment (HEAF) may be mechanisms contributing to the observed cellular resistance to AF.

  7. File list: Oth.Bld.20.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  8. File list: Oth.Bld.50.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  9. File list: Oth.Bld.10.AllAg.MLL-AF9_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Bld.10.AllAg.MLL-AF9_leukemic_cell mm9 TFs and others Blood MLL-AF9 leukemic ce...RX1114547,SRX1114544,SRX376584,SRX099358,SRX475269,SRX734390,SRX734386,SRX734394 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Bld.10.AllAg.MLL-AF9_leukemic_cell.bed ...

  10. File list: ALL.Bld.50.AllAg.MLL-AF10_leukemic_cell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  11. All-trans retinoic acid combined with 5-Aza-2 Prime -deoxycitidine induces C/EBP{alpha} expression and growth inhibition in MLL-AF9-positive leukemic cells

    Energy Technology Data Exchange (ETDEWEB)

    Fujiki, Atsushi [Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan); Imamura, Toshihiko, E-mail: imamura@koto.kpu-m.ac.jp [Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan); Sakamoto, Kenichi; Kawashima, Sachiko; Yoshida, Hideki; Hirashima, Yoshifumi; Miyachi, Mitsuru; Yagyu, Shigeki; Nakatani, Takuya [Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan); Sugita, Kanji [Department of Pediatrics, University of Yamanashi, Yamanashi (Japan); Hosoi, Hajime [Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan)

    2012-11-16

    Highlights: Black-Right-Pointing-Pointer We tested whether ATRA and 5-Aza affect AML cell differentiation and growth. Black-Right-Pointing-Pointer Cell differentiation and growth arrest were induced in MLL-AF9-expressing cells. Black-Right-Pointing-Pointer Increased expression of C/EBP{alpha}, C/EBP{epsilon}, and PU.1 were also observed. Black-Right-Pointing-Pointer MLL-AF4/AF5q31-expressing cells are less sensitive to ATRA and 5-Aza. Black-Right-Pointing-Pointer Different MLL fusion has distinct epigenetic properties related to RA pathway. -- Abstract: The present study tested whether all-trans retinoic acid (ATRA) and 5-Aza-2 Prime -deoxycitidine (5-Aza) affect AML cell differentiation and growth in vitro by acting on the CCAAT/enhancer binding protein {alpha} (C/EBP{alpha}) and c-Myc axis. After exposure to a combination of these agents, cell differentiation and growth arrest were significantly higher in human and murine MLL-AF9-expressing cells than in MLL-AF4/AF5q31-expressing cells, which were partly associated with increased expression of C/EBP{alpha}, C/EBP{epsilon}, and PU.1, and decreased expression of c-Myc. These findings indicate that MLL-AF9-expressing cells are more sensitive to ATRA and 5-Aza, indicating that different MLL fusion proteins possess different epigenetic properties associated with retinoic acid pathway inactivation.

  12. Gold resistance in cultured human cells possible role of metallothionein.

    Science.gov (United States)

    Glennås, A

    1983-01-01

    Insufficient therapeutic effect of auranofin (AF), used in the treatment of rheumatoid arthritis, is found in about 8% of the patients included in clinical trials until now. The mechanisms of resistance to gold-containing drugs are not known, but one reason might be acquired drug resistance. We have studied the relationship between the effects of gold and concentration of the cytoplasmic metal-binding protein metallothionein (MT), in order to evaluate MT as a possible contributing factor to resistance against AF. Different strains of cultured human epithelial cells derived from normal skin, treated with AF, were used as models. The experiments indicate two possible mechanisms for resistance against AF in cells: 1) binding of gold to pre-existent cadmium-induced MT or to de novo AF-induced MT, and 2) the cells' ability to keep the intracellular gold concentration at a low level. AF apparently causes a rapid and pronounced increase of MT-content in these cells. Preliminary results also indicated that AF causes increase of MT-content in human rheumatoid synovial cells, grown as primary cultures. These findings may have two clinical implications: 1) AF-induced MT may decrease therapeutic response, and 2) decrease the toxicity of AF.

  13. Inhibitory and Apoptosis-Inducing Effects of Newcastle Disease Virus Strain AF2240 on Mammary Carcinoma Cell Line

    Directory of Open Access Journals (Sweden)

    Umar Ahmad

    2015-01-01

    Full Text Available Breast cancer is the malignant tumour that developed from cells of the breast and is the first leading cause of cancer death among women worldwide. Surgery, radiotherapy, and chemotherapy are the available treatments for breast cancer, but these were reported to have side effects. Newcastle disease virus (NDV known as Avian paramyxovirus type-1 (APMV1 belongs to the genus Avulavirus in a family Paramyxoviridae. NDV is shown to be a promising anticancer agent, killing tumour cells while sparing normal cells unharmed. In this study, the oncolytic and cytotoxic activities of NDV AF2240 strain were evaluated on MDA-MB-231, human mammary carcinoma cell line, using MTT assay, and its inhibitory effects were further studied using proliferation and migration assays. Morphological and apoptotic-inducing effects of NDV on MD-MB-231 cells were observed using phase contrast and fluorescence microscopes. Detection of DNA fragmentation was done following terminal deoxyribonucleotide transferase-mediated Br-dUTP nick end labeling staining (TUNEL assay, which confirmed that the mode of death was through apoptosis and was quantified by flow cytometry. Furthermore, analysis of cellular DNA content demonstrated that the virus caused an increase in the sub-G1 phase (apoptotic peak of the cell cycle. It appears that NDV AF2240 strain is a potent anticancer agent that induced apoptosis in time-dependent manner.

  14. Two-way cooperative AF relaying in spectrum-sharing systems: Enhancing cell-edge performance

    KAUST Repository

    Xia, Minghua

    2012-09-01

    In this contribution, two-way cooperative amplify-and-forward (AF) relaying technique is integrated into spectrumsharing wireless systems to improve spectral efficiency of secondary users (SUs). In order to share the available spectrum resources originally dedicated to primary users (PUs), the transmit power of a SU is optimized with respect to the average tolerable interference power at primary receivers. By analyzing outage probability and achievable data rate at the base station and at a cell-edge SU, our results reveal that the uplink performance is dominated by the average tolerable interference power at primary receivers, while the downlink always behaves like conventional one-way AF relaying and its performance is dominated by the average signal-to-noise ratio (SNR). These important findings provide fresh perspectives for system designers to improve spectral efficiency of secondary users in next-generation broadband spectrum-sharing wireless systems. © 2012 IEEE.

  15. Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid

    Directory of Open Access Journals (Sweden)

    Sanie-Jahromi Fatemeh

    2012-04-01

    Full Text Available Abstract Background Retinal progenitor cells are a convenient source of cell replacement therapy in retinal degenerative disorders. The purpose of this study was to evaluate the expression patterns of the homeobox genes PAX6 and CHX10 (retinal progenitor markers during treatment of human retinal pigment epithelium (RPE cells with amniotic fluid (AF, RPE cells harvested from neonatal cadaver globes were cultured in a mixture of DMEM and Ham's F12 supplemented with 10% FBS. At different passages, cells were trypsinized and co-cultured with 30% AF obtained from normal fetuses of 1416 weeks gestational age. Results Compared to FBS-treated controls, AF-treated cultures exhibited special morphological changes in culture, including appearance of spheroid colonies, improved initial cell adhesion and ordered cell alignment. Cell proliferation assays indicated a remarkable increase in the proliferation rate of RPE cells cultivated in 30% AF-supplemented medium, compared with those grown in the absence of AF. Immunocytochemical analyses exhibited nuclear localization of retinal progenitor markers at a ratio of 33% and 27% for CHX10 and PAX6, respectively. This indicated a 3-fold increase in retinal progenitor markers in AF-treated cultures compared to FBS-treated controls. Real-time PCR data of retinal progenitor genes (PAX6, CHX10 and VSX-1 confirmed these results and demonstrated AF's capacity for promoting retinal progenitor cell generation. Conclusion Taken together, the results suggest that AF significantly promotes the rate of retinal progenitor cell generation, indicating that AF can be used as an enriched supplement for serum-free media used for the in vitro propagation of human progenitor cells.

  16. Human amniotic fluid stem cells support undifferentiated propagation and pluripotency of human embryonic stem cell without b-FGF in a density dependent manner.

    Science.gov (United States)

    Ma, Xiaorong; Li, Huanqi; Xin, Shujia; Ma, Yueting; Ouyang, Tianxiang

    2014-01-01

    Human embryonic stem cells (hESCs) are pluripotent cells which can give rise to almost all adult cell lineages. Culture system of hESCs is complex, requiring exogenous b-FGF and feeder cell layer. Human mesenchymal stem cells (MSCs) not only synthesize soluble cytokines or factors such as b-FGF, but also provide other mechanism which might play positive role on sustaining hESCs propagation and pluripotency. Human amniotic fluid stem (AFS) cells, which share characteristics of both embryonic and adult stem cells, have been regarded as promising cells for regenerative medicine. Taking advantage by AFS cells, we studied the ability of AFS cells in supporting undifferentiated propagation and pluripotency of Chinese population derived X-01 hESCs. Human AF-type amniotic fluid stem cells (hAF-AFSCs) transcribed genes including Activin A, TGF-β1, Noggin and b-FGF, which involved in maintaining pluripotency and self-renewal of hESCs. Compared to mouse embryonic fibroblasts (MEFs), hAF-AFSCs secreted higher concentration of b-FGF which was important in hESCs culture (P FGF supplementation, keeping undifferentiated status. While exogenous b-FGF was obviated, propagation of hESCs with undifferentiated status was dependent on density of hAF-AFSC feeder layer. Lower density of hAF-AFSCs resulted in rapid decline in undifferentiated clone number, while higher ones hindered the growth of colonies. The most appropriate hAF-AFSCs feeder density to maintain the X-01 hESC line without exogenous b-FGF was 15-20×10(4)/well. To the best of our knowledge, this is the first study demonstrating that hAF-AFSCs could support undifferentiated propagation and pluripotency of Chinese population derived hESCs without exogenous b-FGF supplementation.

  17. Human and murine amniotic fluid c-Kit+Lin- cells display hematopoietic activity.

    Science.gov (United States)

    Ditadi, Andrea; de Coppi, Paolo; Picone, Olivier; Gautreau, Laetitia; Smati, Rim; Six, Emmanuelle; Bonhomme, Delphine; Ezine, Sophie; Frydman, René; Cavazzana-Calvo, Marina; André-Schmutz, Isabelle

    2009-04-23

    We have isolated c-Kit(+)Lin(-) cells from both human and murine amniotic fluid (AF) and investigated their hematopoietic potential. In vitro, the c-Kit(+)Lin(-) population in both species displayed a multilineage hematopoietic potential, as demonstrated by the generation of erythroid, myeloid, and lymphoid cells. In vivo, cells belonging to all 3 hematopoietic lineages were found after primary and secondary transplantation of murine c-Kit(+)Lin(-) cells into immunocompromised hosts, thus demonstrating the ability of these cells to self-renew. Gene expression analysis of c-Kit(+) cells isolated from murine AF confirmed these results. The presence of cells with similar characteristics in the surrounding amnion indicates the possible origin of AF c-Kit(+)Lin(-) cells. This is the first report showing that cells isolated from the AF do have hematopoietic potential; our results support the idea that AF may be a new source of stem cells for therapeutic applications.

  18. The planar cell polarity (PCP) protein Diversin translocates to the nucleus to interact with the transcription factor AF9

    Energy Technology Data Exchange (ETDEWEB)

    Haribaskar, Ramachandran; Puetz, Michael; Schupp, Birte; Skouloudaki, Kassiani; Bietenbeck, Andreas; Walz, Gerd [Renal Division, University Hospital Freiburg, Hugstetter Strasse 55, D-79106 Freiburg (Germany); Schaefer, Tobias, E-mail: tobias.schaefer@uniklinik-freiburg.de [Renal Division, University Hospital Freiburg, Hugstetter Strasse 55, D-79106 Freiburg (Germany)

    2009-09-11

    The planar cell polarity (PCP) pathway, a {beta}-catenin-independent branch of the Wnt signaling pathway, orients cells and their appendages with respect to the body axes. Diversin, the mammalian homolog of the Drosophila PCP protein Diego, acts as a molecular switch that blocks {beta}-catenin-dependent and promotes {beta}-catenin-independent Wnt signaling. We report now that Diversin, containing several nuclear localization signals, translocates to the nucleus, where it interacts with the transcription factor AF9. Both Diversin and AF9 block canonical Wnt signaling; however, this occurs independently of each other, and does not require nuclear Diversin. In contrast, AF9 strongly augments the Diversin-driven activation of c-Jun N-terminal kinase (JNK)-dependent gene expression in the nucleus, and this augmentation largely depends on the presence of nuclear Diversin. Thus, our findings reveal that components of the PCP cascade translocate to the nucleus to participate in transcriptional regulation and PCP signaling.

  19. Activation of Wnt signaling pathway by AF1q enriches stem-like population and enhance mammosphere formation of breast cells.

    Science.gov (United States)

    Tse, Charlotte Olivia; Kim, Soojin; Park, Jino

    2017-03-18

    Wnt signaling pathway is believed to be responsible for control over various types of stem cells and may act as a niche factor to maintain stem cells in a self-renewing state. Moreover, dysregulated Wnt signaling pathway is strongly associated with several diseases including cancer. Previously, we have shown that AF1q associates with a poor prognosis in leukemia, myelodysplastic syndromes, multiple myeloid, ovarian cancer, and breast cancer. Also, AF1q plays a pivotal role as an oncogene and metastasis enhancer in breast cancer via activation of Wnt signaling pathway. AF1q is highly expressed in stem cells, and this expression is diminished by differentiation. To understand the role of AF1q in stem-like population, we examined stem-like cells derived from breast cells which dysregulated Wnt signaling pathway by alteration of AF1q expression. The effect of Wnt signaling pathway by AF1q on EMT marker expression, stem cell marker expression, and sphere formation was determined. Activated Wnt signaling pathway by AF1q enriched stem-like population showed enhanced sphere formation ability. Interestingly, Wnt signaling pathway inhibitor, Quercetin, decreased the sphere formation in these cells. These results suggest that AF1q would have a role as an enhancer in generation of stem-like population through activation of Wnt signaling pathway.

  20. miR-128b is a potent glucocorticoid sensitizer in MLL-AF4 acute lymphocytic leukemia cells and exerts cooperative effects with miR-221.

    Science.gov (United States)

    Kotani, Ai; Ha, Daon; Hsieh, James; Rao, Prakash K; Schotte, Diana; den Boer, Monique L; Armstrong, Scott A; Lodish, Harvey F

    2009-11-05

    MLL-AF4 acute lymphocytic leukemia (ALL) has a poor prognosis. MicroRNAs (miRNA) are small noncoding RNAs that posttranscriptionally regulate expression of target mRNAs. Our analysis of previously published data showed that expression of miR-128b and miR-221 is down-regulated in MLL-rearranged ALL relative to other types of ALL. Reexpression of these miRNAs cooperatively sensitizes 2 cultured lines of MLL-AF4 ALL cells to glucocorticoids. Target genes down-regulated by miR-128b include MLL, AF4, and both MLL-AF4 and AF4-MLL fusion genes; miR-221 down-regulates CDKN1B. These results demonstrate that down-regulation of miR-128b and miR-221 is implicated in glucocorticoid resistance and that restoration of their levels is a potentially promising therapeutic in MLL-AF4 ALL.

  1. Etiske Aspekter Inden For Brugen Af Humane Embryoniske Stamceller til Insulin Fremstillende Forskning

    OpenAIRE

    Kyhnauv, Ida Johanne; Køneke, Casper; Frost, Jeanne Birgit; Berendtsen, Nicolai Tubæk; Knudsen, Peter Alexander

    2016-01-01

    This study focuses on the ethical challenges regarding differentiation and creation of insulin producing cells from human embryonic stem cells (hESCs). We include the development, advantages and disadvantages of using hESCs and induced pluripotent stem cells (iPSCs). We outline the ethical aspects concerning the use of hESC in research, based on our case as well as ethical and biological theories on the making of hESCs and iPSCs. We likewise include legislation and articles, where ethnical di...

  2. LCA af genbrug af mursten

    DEFF Research Database (Denmark)

    Møller, Jacob; Damgaard, Anders; Astrup, Thomas Fruergaard

    Der er gennemført en livscyklusbaseret miljøvurdering af genbrug af mursten. Modelleringen er baseret på, hvordan Virksomheden ”Gamle Mursten” forbereder de gamle mursten med henblik på, at de kan genbruges i nybyggeri. Som alternativ til direkte genbrug nedknuses murstensaffaldet og anvendes som...

  3. Short Interspersed Nuclear Element (SINE) Sequences in the Genome of the Human Pathogenic Fungus Aspergillus fumigatus Af293.

    Science.gov (United States)

    Kanhayuwa, Lakkhana; Coutts, Robert H A

    2016-01-01

    Novel families of short interspersed nuclear element (SINE) sequences in the human pathogenic fungus Aspergillus fumigatus, clinical isolate Af293, were identified and categorised into tRNA-related and 5S rRNA-related SINEs. Eight predicted tRNA-related SINE families originating from different tRNAs, and nominated as AfuSINE2 sequences, contained target site duplications of short direct repeat sequences (4-14 bp) flanking the elements, an extended tRNA-unrelated region and typical features of RNA polymerase III promoter sequences. The elements ranged in size from 140-493 bp and were present in low copy number in the genome and five out of eight were actively transcribed. One putative tRNAArg-derived sequence, AfuSINE2-1a possessed a unique feature of repeated trinucleotide ACT residues at its 3'-terminus. This element was similar in sequence to the I-4_AO element found in A. oryzae and an I-1_AF long nuclear interspersed element-like sequence identified in A. fumigatus Af293. Families of 5S rRNA-related SINE sequences, nominated as AfuSINE3, were also identified and their 5'-5S rRNA-related regions show 50-65% and 60-75% similarity to respectively A. fumigatus 5S rRNAs and SINE3-1_AO found in A. oryzae. A. fumigatus Af293 contains five copies of AfuSINE3 sequences ranging in size from 259-343 bp and two out of five AfuSINE3 sequences were actively transcribed. Investigations on AfuSINE distribution in the fungal genome revealed that the elements are enriched in pericentromeric and subtelomeric regions and inserted within gene-rich regions. We also demonstrated that some, but not all, AfuSINE sequences are targeted by host RNA silencing mechanisms. Finally, we demonstrated that infection of the fungus with mycoviruses had no apparent effects on SINE activity.

  4. Comparison of the neural differentiation potential of human mesenchymal stem cells from amniotic fluid and adult bone marrow.

    Science.gov (United States)

    Yan, Zhong-Jie; Hu, Yu-Qin; Zhang, Hong-Tian; Zhang, Peng; Xiao, Zong-Yu; Sun, Xin-Lin; Cai, Ying-Qian; Hu, Chang-Chen; Xu, Ru-Xiang

    2013-05-01

    Human mesenchymal stem cells (MSCs) are considered a promising tool for cell-based therapies of nervous system diseases. Bone marrow (BM) has been the traditional source of MSCs (BM-MSCs). However, there are some limitations for their clinical use, such as the decline in cell number and differentiation potential with age. Recently, amniotic fluid (AF)-derived MSCs (AF-MSCs) have been shown to express embryonic and adult stem cell markers, and can differentiate into cells of all three germ layers. In this study, we isolated AF-MSCs from second-trimester AF by limiting dilution and compared their proliferative capacity, multipotency, neural differentiation ability, and secretion of neurotrophins to those of BM-MSCs. AF-MSCs showed a higher proliferative capacity and more rapidly formed and expanded neurospheres compared to those of BM-MSCs. Both immunocytochemical and quantitative real-time PCR analyses demonstrated that AF-MSCs showed higher expression of neural stemness markers than those of BM-MSCs following neural stem cell (NSC) differentiation. Furthermore, the levels of brain-derived growth factor and nerve growth factor secreted by AF-MSCs in the culture medium were higher than those of BM-MSCs. In addition, AF-MSCs maintained a normal karyotype in long-term cultures after NSC differentiation and were not tumorigenic in vivo. Our findings suggest that AF-MSCs are a promising and safe alternative to BM-MSCs for therapy of nervous system diseases.

  5. Boganmeldelse af ”biologisk antropologi med human osteologi” Red. Niels Lynnerup, Pia Bennike og Elisabeth Iregren. Forlag Gyldendal 2008

    DEFF Research Database (Denmark)

    Leth, Peter Mygind

    2009-01-01

    Vi har i Norden længe savnet en samlet og moderne fremstilling af biologisk antropologi set fra en skandinavisk synsvinkel. Den nye lærebog i biologisk antropologi opfylder dette behov. Den er skrevet af førende forskere fra de skandinaviske lande samt enkelte andre. Bogens opbygning er logisk og...

  6. Boganmeldelse af ”biologisk antropologi med human osteologi” Red. Niels Lynnerup, Pia Bennike og Elisabeth Iregren. Forlag Gyldendal 2008

    DEFF Research Database (Denmark)

    Leth, Peter Mygind

    2009-01-01

    Vi har i Norden længe savnet en samlet og moderne fremstilling af biologisk antropologi set fra en skandinavisk synsvinkel. Den nye lærebog i biologisk antropologi opfylder dette behov. Den er skrevet af førende forskere fra de skandinaviske lande samt enkelte andre. Bogens opbygning er logisk og...

  7. Disrupted interaction between CFTR and AF-6/afadin aggravates malignant phenotypes of colon cancer.

    Science.gov (United States)

    Sun, Ting Ting; Wang, Yan; Cheng, Hong; Xiao, Hu Zhang; Xiang, Juan Juan; Zhang, Jie Ting; Yu, Siu Bun Sydney; Martin, Tracey Amanda; Ye, Lin; Tsang, Lai Ling; Jiang, Wen Guo; Xiaohua, Jiang; Chan, Hsiao Chang

    2014-03-01

    How mutations or dysfunction of CFTR may increase the risk of malignancies in various tissues remains an open question. Here we report the interaction between CFTR and an adherens junction molecule, AF-6/afadin, and its involvement in the development of colon cancer. We have found that CFTR and AF-6/afadin are co-localized at the cell-cell contacts and physically interact with each other in colon cancer cell lines. Knockdown of CFTR results in reduced epithelial tightness and enhanced malignancies, with increased degradation and reduced stability of AF-6/afadin protein. The enhanced invasive phenotype of CFTR-knockdown cells can be completely reversed by either AF-6/afadin over-expression or ERK inhibitor, indicating the involvement of AF-6/MAPK pathway. More interestingly, the expression levels of CFTR and AF-6/afadin are significantly downregulated in human colon cancer tissues and lower expression of CFTR and/or AF-6/afadin is correlated with poor prognosis of colon cancer patients. The present study has revealed a previously unrecognized interaction between CFTR and AF-6/afadin that is involved in the pathogenesis of colon cancer and indicated the potential of the two as novel markers of metastasis and prognostic predictors for human colon cancer.

  8. Isolation of c-Kit+ human amniotic fluid stem cells from second trimester.

    Science.gov (United States)

    Pozzobon, Michela; Piccoli, Martina; Schiavo, Andrea Alex; Atala, Anthony; De Coppi, Paolo

    2013-01-01

    Amniotic fluid-derived stem (AFS) cells have been described as an appealing source of stem cells because of their (1) fetal, non-embryonic origin, (2) easy access during pregnancy overcoming the ethical issues related both to the use of human embryonic cells and to the postnatal tissue biopsy with donor site morbidity, and (3) their undemanding ability to be expanded. We and others have demonstrated the broad differentiation potential and here we describe the established protocol we developed to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.

  9. The prognosis of CALM-AF10-positive adult T-cell acute lymphoblastic leukemias depends on the stage of maturation arrest

    Science.gov (United States)

    Ben Abdelali, Raouf; Asnafi, Vahid; Petit, Arnaud; Micol, Jean-Baptiste; Callens, Céline; Villarese, Patrick; Delabesse, Eric; Reman, Oumedaly; Lepretre, Stephane; Cahn, Jean-Yves; Guillerm, Gaelle; Berthon, Céline; Gardin, Claude; Corront, Bernadette; Leguay, Thibaut; Béné, Marie-Christine; Ifrah, Norbert; Leverger, Guy; Dombret, Hervé; Macintyre, Elizabeth

    2013-01-01

    CALM-AF10 (also known as PICALM-MLLT10) is the commonest fusion protein in T-cell acute lymphoblastic leukemia, but its prognostic impact remains unclear. Molecular screening at diagnosis identified CALM-AF10 in 30/431 (7%) patients with T-cell acute lymphoblastic leukemia aged 16 years and over and in 15/234 (6%) of those aged up to 15 years. Adult CALM-AF10-positive patients were predominantly (72%) negative for surface (s)CD3/T-cell receptor, whereas children were predominantly (67%) positive for T-cell receptor. Among 22 adult CALM-AF10-positive patients treated according to the LALA94/GRAALL03-05 protocols, the poor prognosis for event-free survival (P=0.0017) and overall survival (P=0.0014) was restricted to the 15 T-cell receptor-negative cases. Among CALM-AF10-positive, T-cell receptor-negative patients, 82% had an early T-cell precursor phenotype, reported to be of poor prognosis in pediatric T-cell acute lymphoblastic leukemia. Early T-cell precursor acute lymphoblastic leukemia corresponded to 22% of adult LALA94/GRAALL03-05 T-cell acute lymphoblastic leukemias, but had no prognostic impact per se. CALM-AF10 fusion within early T-cell precursor acute lymphoblastic leukemia (21%) did, however, identify a group with a poor prognosis with regards to event-free survival (P=0.04). CALM-AF10 therefore identifies a poor prognostic group within sCD3/T-cell receptor negative adult T-cell acute lymphoblastic leukemias and is over-represented within early T-cell precursor acute lymphoblastic leukemias, in which it identifies patients in whom treatment is likely to fail. Its prognosis and overlap with early T-cell precursor acute lymphoblastic leukemia in pediatric T-cell acute lymphoblastic leukemia merits analysis. The clinical trial GRAALL was registered at Clinical Trials.gov number NCT00327678. PMID:23831922

  10. Binding of hnRNP H and U2AF65 to respective G-codes and a poly-uridine tract collaborate in the N50-5'ss selection of the REST N exon in H69 cells.

    Science.gov (United States)

    Ortuño-Pineda, Carlos; Galindo-Rosales, José Manuel; Calderón-Salinas, José Victor; Villegas-Sepúlveda, Nicolás; Saucedo-Cárdenas, Odila; De Nova-Ocampo, Mónica; Valdés, Jesús

    2012-01-01

    The splicing of the N exon in the pre-mRNA coding for the RE1-silencing transcription factor (REST) results in a truncated protein that modifies the expression pattern of some of its target genes. A weak 3'ss, three alternative 5'ss (N4-, N50-, and N62-5'ss) and a variety of putative target sites for splicing regulatory proteins are found around the N exon; two GGGG codes (G2-G3) and a poly-Uridine tract (N-PU) are found in front of the N50-5'ss. In this work we analyzed some of the regulatory factors and elements involved in the preferred selection of the N50-5'ss (N50 activation) in the small cell lung cancer cell line H69. Wild type and mutant N exon/β-globin minigenes recapitulated N50 exon splicing in H69 cells, and showed that the N-PU and the G2-G3 elements are required for N50 exon splicing. Biochemical and knockdown experiments identified these elements as U2AF65 and hnRNP H targets, respectively, and that they are also required for N50 exon activation. Compared to normal MRC5 cells, and in keeping with N50 exon activation, U2AF65, hnRNP H and other splicing factors were highly expressed in H69 cells. CLIP experiments revealed that hnRNP H RNA-binding occurs first and is a prerequisite for U2AF65 RNA binding, and EMSA and CLIP experiments suggest that U2AF65-RNA recognition displaces hnRNP H and helps to recruit other splicing factors (at least U1 70K) to the N50-5'ss. Our results evidenced novel hnRNP H and U2AF65 functions: respectively, U2AF65-recruiting to a 5'ss in humans and the hnRNP H-displacing function from two juxtaposed GGGG codes.

  11. Binding of hnRNP H and U2AF65 to respective G-codes and a poly-uridine tract collaborate in the N50-5'ss selection of the REST N exon in H69 cells.

    Directory of Open Access Journals (Sweden)

    Carlos Ortuño-Pineda

    Full Text Available The splicing of the N exon in the pre-mRNA coding for the RE1-silencing transcription factor (REST results in a truncated protein that modifies the expression pattern of some of its target genes. A weak 3'ss, three alternative 5'ss (N4-, N50-, and N62-5'ss and a variety of putative target sites for splicing regulatory proteins are found around the N exon; two GGGG codes (G2-G3 and a poly-Uridine tract (N-PU are found in front of the N50-5'ss. In this work we analyzed some of the regulatory factors and elements involved in the preferred selection of the N50-5'ss (N50 activation in the small cell lung cancer cell line H69. Wild type and mutant N exon/β-globin minigenes recapitulated N50 exon splicing in H69 cells, and showed that the N-PU and the G2-G3 elements are required for N50 exon splicing. Biochemical and knockdown experiments identified these elements as U2AF65 and hnRNP H targets, respectively, and that they are also required for N50 exon activation. Compared to normal MRC5 cells, and in keeping with N50 exon activation, U2AF65, hnRNP H and other splicing factors were highly expressed in H69 cells. CLIP experiments revealed that hnRNP H RNA-binding occurs first and is a prerequisite for U2AF65 RNA binding, and EMSA and CLIP experiments suggest that U2AF65-RNA recognition displaces hnRNP H and helps to recruit other splicing factors (at least U1 70K to the N50-5'ss. Our results evidenced novel hnRNP H and U2AF65 functions: respectively, U2AF65-recruiting to a 5'ss in humans and the hnRNP H-displacing function from two juxtaposed GGGG codes.

  12. Do mechanical strain and TNF-α interact to amplify pro-inflammatory cytokine production in human annulus fibrosus cells?

    Science.gov (United States)

    Likhitpanichkul, Morakot; Torre, Olivia M; Gruen, Jadry; Walter, Benjamin A; Hecht, Andrew C; Iatridis, James C

    2016-05-03

    During intervertebral disc (IVD) injury and degeneration, annulus fibrosus (AF) cells experience large mechanical strains in a pro-inflammatory milieu. We hypothesized that TNF-α, an initiator of IVD inflammation, modifies AF cell mechanobiology via cytoskeletal changes, and interacts with mechanical strain to enhance pro-inflammatory cytokine production. Human AF cells (N=5, Thompson grades 2-4) were stretched uniaxially on collagen-I coated chambers to 0%, 5% (physiological) or 15% (pathologic) strains at 0.5Hz for 24h under hypoxic conditions with or without TNF-α (10ng/mL). AF cells were treated with anti-TNF-α and anti-IL-6. ELISA assessed IL-1β, IL-6, and IL-8 production and immunocytochemistry measured F-actin, vinculin and α-tubulin in AF cells. TNF-α significantly increased AF cell pro-inflammatory cytokine production compared to basal conditions (IL-1β:2.0±1.4-84.0±77.3, IL-6:10.6±9.9-280.9±214.1, IL-8:23.9±26.0-5125.1±4170.8pg/ml for basal and TNF-α treatment, respectively) as expected, but mechanical strain did not. Pathologic strain in combination with TNF-α increased IL-1β, and IL-8 but not IL-6 production of AF cells. TNF-α treatment altered F-actin and α-tubulin in AF cells, suggestive of altered cytoskeletal stiffness. Anti-TNF-α (infliximab) significantly inhibited pro-inflammatory cytokine production while anti-IL-6 (atlizumab) did not. In conclusion, TNF-α altered AF cell mechanobiology with cytoskeletal remodeling that potentially sensitized AF cells to mechanical strain and increased TNF-α-induced pro-inflammatory cytokine production. Results suggest an interaction between TNF-α and mechanical strain and future mechanistic studies are required to validate these observations.

  13. Restaurering af Skjern Å

    DEFF Research Database (Denmark)

    Jessen, K.; Boysen Larsen, B.; Bundgaard, P.;

    Denne rapport indeholder resultaterne af den natur- og miljøovervågning, der blev iværksat som en del af naturgenopretningen af Skjern Å 1999-2002. Ved projektet ophørte den kunstige afvanding af den vestlige del af Skjern Å dalen. Størstedelen af de dyrkede arealer ændredes til ekstensive græsni...

  14. Serum-Free Cryopreservation of Human Amniotic Epithelial Cells

    Directory of Open Access Journals (Sweden)

    H. Niknejad

    2013-04-01

    Full Text Available Introduction & Objective: One of the important issues in long term storage of cells is removal of animal serum from cell culture environments. The aim of this study was to evaluate amni-otic fluid (AF, which is full of growth factors, as substitute for fetal bovine serum (FBS in the cryopreservation protocol. Materials & Methods: In this experimental study human amniotic epithelial cells were isolated from placentas which were seronegative for microbial infections. The cells were preserved in 24 different patterns for 12 months in -196 ?C (liquid nitrogen and viability of cells were determined before and after cryopreservation by trypan blue and MTT assay. Moreover, Oct-4 expression was studied to determine pluripotency before and after cryopreservation with immunocytochemistry. Results were compared between groups with ANOVA (Tukey Post-Test. P.value under 0.01 and 0.05 was considered statistically significant. Results: The presence of DMEM, FBS or AF is necessary for amniotic cell cryopreservation. Trypan-blue, MTT and immunocytochemistry showed that there isn’t significant difference between using AF and FBS in viability and pluripotency of cells. Moreover, results showed that DMSO is a better cryoprotectant compared to glycerol. Conclusion : Results showed that amniotic fluid can be a proper substitute for FBS in amniotic epithelial cells cryopreservation. (Sci J Hamadan Univ Med Sci 2013; 20 (1:15-24

  15. Internationalisering af servicedesign

    DEFF Research Database (Denmark)

    Henten, Anders

    Rapporten omhandler internationaliseringen af designvirksomhede med special fokus på servicedesign.......Rapporten omhandler internationaliseringen af designvirksomhede med special fokus på servicedesign....

  16. Human amniotic fluid stem cells as a model for functional studies of genes involved in human genetic diseases or oncogenesis.

    Science.gov (United States)

    Rosner, Margit; Dolznig, Helmut; Schipany, Katharina; Mikula, Mario; Brandau, Oliver; Hengstschläger, Markus

    2011-09-01

    Besides their putative usage for therapies, stem cells are a promising tool for functional studies of genes involved in human genetic diseases or oncogenesis. For this purpose induced pluripotent stem (iPS) cells can be derived from patients harbouring specific mutations. In contrast to adult stem cells, iPS cells are pluripotent and can efficiently be grown in culture. However, iPS cells are modulated due to the ectopic induction of pluripotency, harbour other somatic mutations accumulated during the life span of the source cells, exhibit only imperfectly cleared epigenetic memory of the source cell, and are often genomically instable. In addition, iPS cells from patients only allow the investigation of mutations, which are not prenatally lethal. Embryonic stem (ES) cells have a high proliferation and differentiation potential, but raise ethical issues. Human embryos, which are not transferred in the course of in vitro fertilization, because of preimplantation genetic diagnosis of a genetic defect, are still rarely donated for the establishment of ES cell lines. In addition, their usage for studies on gene functions for oncogenesis is hampered by the fact the ES cells are already tumorigenic per se. In 2003 amniotic fluid stem (AFS) cells have been discovered, which meanwhile have been demonstrated to harbour the potential to differentiate into cells of all three germ layers. Monoclonal human AFS cell lines derived from amniocenteses have a high proliferative potential, are genomically stable and are not associated with ethical controversies. Worldwide amniocenteses are performed for routine human genetic diagnosis. We here discuss how generation and banking of monoclonal human AFS cell lines with specific chromosomal aberrations or monogenic disease mutations would allow to study the functional consequences of disease causing mutations. In addition, recently a protocol for efficient and highly reproducible siRNA-mediated long-term knockdown of endogenous gene

  17. Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases

    Directory of Open Access Journals (Sweden)

    Ivana Antonucci

    2016-04-01

    Full Text Available In recent years, great interest has been devoted to the use of Induced Pluripotent Stem cells (iPS for modeling of human genetic diseases, due to the possibility of reprogramming somatic cells of affected patients into pluripotent cells, enabling differentiation into several cell types, and allowing investigations into the molecular mechanisms of the disease. However, the protocol of iPS generation still suffers from technical limitations, showing low efficiency, being expensive and time consuming. Amniotic Fluid Stem cells (AFS represent a potential alternative novel source of stem cells for modeling of human genetic diseases. In fact, by means of prenatal diagnosis, a number of fetuses affected by chromosomal or Mendelian diseases can be identified, and the amniotic fluid collected for genetic testing can be used, after diagnosis, for the isolation, culture and differentiation of AFS cells. This can provide a useful stem cell model for the investigation of the molecular basis of the diagnosed disease without the necessity of producing iPS, since AFS cells show some features of pluripotency and are able to differentiate in cells derived from all three germ layers “in vitro”. In this article, we describe the potential benefits provided by using AFS cells in the modeling of human genetic diseases.

  18. Human mesenchymal stem cells

    DEFF Research Database (Denmark)

    Abdallah, Basem; Kassem, Moustapha

    2008-01-01

    Mesenchymal stem cells (MSC) are a group of clonogenic cells present among the bone marrow stroma and capable of multilineage differentiation into mesoderm-type cells such as osteoblasts, adipocytes and chondrocytes. Due to their ease of isolation and their differentiation potential, MSC are being...... introduced into clinical medicine in variety of applications and through different ways of administration. Here, we discuss approaches for isolation, characterization and directing differentiation of human mesenchymal stem cells (hMSC). An update of the current clinical use of the cells is also provided....

  19. Om distributionen af hvidhed

    DEFF Research Database (Denmark)

    Frello, Birgitta

    2007-01-01

    Med udgangspunkt i John Fords Western, Forfølgeren fra 1956, præsenteres en analyse af repræsentationer af race og overskridelse af raceskel, særligt i relation til konstitueringen af subjektpositioner, hvorfra spørgsmål om hvidhed og ikke-hvidhed kan afgøres....

  20. Lyden-af-Leg

    DEFF Research Database (Denmark)

    Toft, Herdis

    Præsentation af seniorforsker-projekt Lyden-af-Leg i et traderingsperspektiv og med indledende fokus på YouTube som traderings-platform.......Præsentation af seniorforsker-projekt Lyden-af-Leg i et traderingsperspektiv og med indledende fokus på YouTube som traderings-platform....

  1. Undervisning af tosprogede elever

    DEFF Research Database (Denmark)

    Horst, Christian

    2003-01-01

    Artiklen fremdrager hovedresultaterne fra Virginia P. Collier's og Wayne P. Thomas's længdeundersøgelser af tosprogede elever i USA, som formentlig er de mest omfattende undersøgelser af undervisningen af tosprogede elever overhovedet. Resultaterne diskuteres i relation til udviklingen af en...

  2. Pathogenetic, Clinical, and Prognostic Features of Adult t(4;11)(q21;q23)/MLL-AF4 Positive B-Cell Acute Lymphoblastic Leukemia

    OpenAIRE

    Marchesi, F.; Girardi, K.; Avvisati, G.

    2011-01-01

    Translocation t(4;11)(q21;q23) leading to formation of MLL-AF4 fusion gene is found in about 10% of newly diagnosed B-cell acute lymphoblastic leukemia (ALL) in adult patients. Patients expressing this chromosomal aberration present typical biological, immunophenotypic, and clinical features. This form of leukemia is universally recognized as high-risk leukemia and treatment intensification with allogeneic hematopoietic stem cell transplantation (HSCT) in first complete remission (CR) could b...

  3. A novel method for generating xeno-free human feeder cells for human embryonic stem cell culture.

    Science.gov (United States)

    Meng, Guoliang; Liu, Shiying; Krawetz, Roman; Chan, Michael; Chernos, Judy; Rancourt, Derrick E

    2008-06-01

    Long-term cultures of human embryonic stem (hES) cells require a feeder layer for maintaining cells in an undifferentiated state and increasing karyotype stability. In routine hES cell culture, mouse embryonic fibroblast (MEF) feeders and animal component-containing media (FBS or serum replacement) are commonly used. However, the use of animal materials increases the risk of transmitting pathogens to hES cells and therefore is not optimal for use in cultures intended for human transplantation. There are other limitations with conventional feeder cells, such as MEFs, which have a short lifespan and can only be propagated five to six passages before senescing. Several groups have investigated maintaining existing hES cell lines and deriving new hES cell lines on human feeder layers. However, almost all of these human source feeder cells employed in previous studies were derived and cultured in animal component conditions. Even though one group previously reported the derivation and culture of human foreskin fibroblasts (HFFs) in human serum-containing medium, this medium is not optimal because HFFs routinely undergo senescence after 10 passages when cultured in human serum. In this study we have developed a completely animal-free method to derive HFFs from primary tissues. We demonstrate that animal-free (AF) HFFs do not enter senescence within 55 passages when cultured in animal-free conditions. This methodology offers alternative and completely animal-free conditions for hES cell culture, thus maintaining hES cell morphology, pluripotency, karyotype stability, and expression of pluripotency markers. Moreover, no difference in hES cell maintenance was observed when they were cultured on AF-HFFs of different passage number or independent derivations.

  4. Lyden af musik - er lyden af idealisme

    DEFF Research Database (Denmark)

    Eigtved, Michael

    2015-01-01

    Programartiklen til Det Ny Teaters opsætning af Rodgers & Hammersteins The Sound of Music, undersøger musicalgenrens - og især de konkrete ophavsmænds- affinitet til fremstillingen af såvel idealistiske som ideologiske universer.......Programartiklen til Det Ny Teaters opsætning af Rodgers & Hammersteins The Sound of Music, undersøger musicalgenrens - og især de konkrete ophavsmænds- affinitet til fremstillingen af såvel idealistiske som ideologiske universer....

  5. Type I IFN signaling constrains IL-17A/F secretion by gammadelta T cells during bacterial infections.

    Science.gov (United States)

    Henry, Thomas; Kirimanjeswara, Girish S; Ruby, Thomas; Jones, Jonathan W; Peng, Kaitian; Perret, Magali; Ho, Lena; Sauer, John-Demian; Iwakura, Yoichiro; Metzger, Dennis W; Monack, Denise M

    2010-04-01

    Recognition of intracellular bacteria by macrophages leads to secretion of type I IFNs. However, the role of type I IFN during bacterial infection is still poorly understood. Francisella tularensis, the causative agent of tularemia, is a pathogenic bacterium that replicates in the cytosol of macrophages leading to secretion of type I IFN. In this study, we investigated the role of type I IFNs in a mouse model of tularemia. Mice deficient for type I IFN receptor (IFNAR1(-/-)) are more resistant to intradermal infection with F. tularensis subspecies novicida (F. novicida). Increased resistance to infection was associated with a specific increase in IL-17A/F and a corresponding expansion of an IL-17A(+) gammadelta T cell population, indicating that type I IFNs negatively regulate the number of IL-17A(+) gammadelta T cells during infection. Furthermore, IL-17A-deficient mice contained fewer neutrophils compared with wild-type mice during infection, indicating that IL-17A contributes to neutrophil expansion during F. novicida infection. Accordingly, an increase in IL-17A in IFNAR1(-/-) mice correlated with an increase in splenic neutrophil numbers. Similar results were obtained in a mouse model of pneumonic tularemia using the highly virulent F. tularensis subspecies tularensis SchuS4 strain and in a mouse model of systemic Listeria monocytogenes infection. Our results indicate that the type I IFN-mediated negative regulation of IL-17A(+) gammadelta T cell expansion is conserved during bacterial infections. We propose that this newly described activity of type I IFN signaling might participate in the resistance of the IFNAR1(-/-) mice to infection with F. novicida and other intracellular bacteria.

  6. Type I Interferon signaling constrains IL-17A/F secretion by γδ T cells during bacterial infections

    Science.gov (United States)

    Henry, Thomas; Kirimanjeswara, Girish S.; Ruby, Thomas; Jones, Jonathan W.; Peng, Kaitian; Perret, Magali; Ho, Lena; Sauer, John-Demian; Iwakura, Yoichiro; Metzger, Dennis W.; Monack, Denise M.

    2010-01-01

    Recognition of intracellular bacteria by macrophages leads to secretion of type I Interferons. However, the role of type I IFN during bacterial infection is still poorly understood. Francisella tularensis, the causative agent of tularemia, is a pathogenic bacterium that replicates in the cytosol of macrophages leading to secretion of type I IFN. Here, we investigated the role of type I IFN in a mouse model of tularemia. Mice deficient for type I IFN receptor (IFNAR1−/−) are more resistant to intradermal infection with F. tularensis subspecies novicida (F. novicida). Increased resistance to infection was associated with a specific increase in IL-17A/F and a corresponding expansion of an IL-17A+ γδ T cell population, indicating that type I IFN negatively regulate the number of IL-17A+ γδ T cells during infection. Furthermore, IL-17A-deficient mice contained fewer neutrophils compared to WT mice upon infection, indicating that IL-17A contributes to neutrophil expansion during F. novicida infection. Accordingly, an increase in IL-17A in IFNAR1−/− mice correlated with an increase in splenic neutrophil numbers. Similar results were obtained in a mouse model of pneumonic tularemia using the highly virulent Francisella tularensis subspecies tularensis SchuS4 strain and in a mouse model of systemic Listeria monocytogenes infection. Our results indicate that the type I IFN-mediated negative regulation of IL-17A+ γδ T cell expansion is conserved during bacterial infections. We propose that this newly described activity of type I IFN signaling might participate in the resistance of the IFNAR1−/− mice to infection with F. novicida and other intracellular bacteria. PMID:20176744

  7. Pathogenetic, Clinical, and Prognostic Features of Adult t(4;11(q21;q23/MLL-AF4 Positive B-Cell Acute Lymphoblastic Leukemia

    Directory of Open Access Journals (Sweden)

    F. Marchesi

    2011-01-01

    Full Text Available Translocation t(4;11(q21;q23 leading to formation of MLL-AF4 fusion gene is found in about 10% of newly diagnosed B-cell acute lymphoblastic leukemia (ALL in adult patients. Patients expressing this chromosomal aberration present typical biological, immunophenotypic, and clinical features. This form of leukemia is universally recognized as high-risk leukemia and treatment intensification with allogeneic hematopoietic stem cell transplantation (HSCT in first complete remission (CR could be a valid option to improve prognosis, but data obtained from the literature are controversial. In this review, we briefly describe pathogenetic, clinical, and prognostic characteristics of adult t(4;11(q21;q23/MLL-AF4 positive ALL and provide a review of the clinical outcome reported by the most important cooperative groups worldwide.

  8. Acceleration of Regeneration of Large-Gap Peripheral Nerve Injuries Using Acellular Nerve Allografts Plus Amniotic Fluid Derived Stem Cells (AFS)

    Science.gov (United States)

    2014-09-01

    nerves are incorporated into other rat strains without immunologic disturbances according to the vendor (AxoGen). 15. SUBJECT TERMS 16. SECURITY...mm). The outcomes of these surgeries will be compared to those obtained with autograft nerve repairs that currently have the best outcomes for large...ANA construct with AFS cells (Group 2), or with an autograft (nerve segment is cut out, reversed, and sewn back in place)(Group 3). All surgeries

  9. Acceleration of Regeneration of Large-Gap Peripheral Nerve Injuries Using Accellular Nerve Allografts Plus Amniotic Fluid Derived Stem Cells (AFS)

    Science.gov (United States)

    2015-09-01

    2 AD______________ AWARD NUMBER: W81XWH-13-1-0310 TITLE: Acceleration of Regeneration of Large-Gap Peripheral Nerve Injuries Using Acellular... Nerve Allografts plus amniotic Fluid Derived Stem Cells (AFS). PRINCIPAL INVESTIGATOR: Li, Zhongyu CONTRACTING ORGANIZATION: Wake Forest...DATE September 2015 2. REPORT TYPE Annual Report 3. DATES COVERED 1 Sep 2014 - 31 Aug 2015 4. TITLE AND SUBTITLE Acceleration of Regeneration of Large

  10. Skoling af lyst

    DEFF Research Database (Denmark)

    Bjerg, Helle

    2011-01-01

    På baggrund af interview med tre elevgenerationer fremanalyseres skolens virkningsfuldhed i skoling af lyst i et historisk perspektiv. I afhandlingen udvikles to teoretisk-analytiske optikker: Skolen som ideologisk rum - med inspiration fra Slavoj Zizeks ideologikritik, samt Skolen som affektivt...

  11. Bliv fan af AUB

    DEFF Research Database (Denmark)

    Hansen, Karsten Kryger

    2010-01-01

    Kort gennemgang af hvad det har betydet for Aalborg Universitetsbibliotek (AUB) at gå på Facebook.......Kort gennemgang af hvad det har betydet for Aalborg Universitetsbibliotek (AUB) at gå på Facebook....

  12. An Immunocompetent Mouse Model for MLL/AF9 Leukemia Reveals the Potential of Spontaneous Cytotoxic T-Cell Response to an Antigen Expressed in Leukemia Cells.

    Directory of Open Access Journals (Sweden)

    Kana Hasegawa

    Full Text Available Leukemia differs substantially with respect to stromal milieu from tumors that progress locally as solid masses, and the physiological importance of immunosurveillance in leukemia remains unclear. However, currently available mouse leukemia models have critical limitations in the context of analyzing immunological regulation of leukemia development. In this study, we transferred mouse MLL/AF9 leukemia-initiating cells into immunocompetent recipient mice without any pre-conditioning such as irradiation, and then analyzed the spontaneous T cell response to an immunogenic antigen expressed in leukemia cells. When the minimum numbers of leukemia-initiating cells for engraftment were transferred, leukemia cells were eradicated by the adaptive immune response in most, if not all, wild-type mice, but not in Rag2-/- recipient mice, which lack adaptive immunity. By contrast, mice transplanted with larger numbers of leukemia cells always developed leukemia. In mice with advanced leukemia, antigen-specific CTLs were also expanded, but were unresponsive to antigen stimulation and expressed high levels of PD-1 and LAG-3. These results provide the first clear demonstration that the spontaneous CTL response to a tumor-cell antigen has the potential to eradicate leukemia, whereas antigen-specific CTLs are exhausted in animals with advanced leukemia. This immunocompetent mouse leukemia model provides a useful platform for developing effective immunotherapies against leukemia.

  13. Stability and sealing of PEC solar cells; Stabilitet og forsegling af PEC solceller

    Energy Technology Data Exchange (ETDEWEB)

    Lauritzen, H.

    2009-06-15

    A requirement for success of the DSC technology on the market for building-integrated PV is visually attractive DSC panels holding a lifetime comparable to alternative facade materials and delivering electricity at reasonable prices. This project concerns the stability and durability of the DSC panel as these are decisive factors for the lifetime of the panels and the price of the produced electricity. The largest challenge of the DSC sealing is the high-performing well-documented - but also aggressive and volatile - iodine/triiodide electrolyte. The heat-sensitive Ru-dyes represent another challenge, as the dye should not be exposed to temperatures above approx. 100 deg. C during the sealing process. Despite the fact that less aggressive electrolytes and more stable dyes are highly focused R and D tasks, the work in the actual project has been carried out according to a 'worst case' strategy, i.e. the search has been for a sealing which is compatible with the iodine/triiodide electrolyte and the Ru-dyes. The rationale behind this strategy is the demanding lifetime requirement s for building-integrated products, which requires the ultimate sealing. In addition to the focus on the DSC sealing, work has been dedicated to the development of a more encapsulation-friendly electrolyte, in which the electrolyte's volatile component has been replaced by an ionic liquid. The ultimate result of the project is an improved polymer edge sealing for DSC cells. The sealant is - contrary to Surlyn and Bynel - stable in contact with the aggressive iodine/ triiodide electrolyte. The sealing is formed by lamination at elevated temperature and under controlled conditions. The robustness and capacity of the sealing process need to be further improved in order to fulfill the laboratories ambitions with regard to volume production of DSC cells and modules. Furthermore should the new sealing be compared both stability-wise and cost-wise with the lead-free solder glass

  14. Sociale konsekvenser af byfornyelsen

    DEFF Research Database (Denmark)

    Andersen, Hans Skifter; Ærø, Thorkild

    byfornyede boliger. Kun meget få flytter dog som følge af byfornyelsen, men af andre grunde. I perioden efter byfornyelsen er der imidlertid en del beboere som vælger at flytte på grund af stigende huslejer. Byfornyelsen betyder, at beboersammensætningen ændres i retning af flere i beskæftigelse med høj...

  15. En verden af medier

    DEFF Research Database (Denmark)

    Tække, Jesper

    2009-01-01

    Bogen: 'En verden af medier – Medialiseringen af politik, sprog, religion og leg' anmeldes. Anmeldelsen kan læses på: http://ojs.statsbiblioteket.dk/index.php/mediekultur/article/view/1370/1499 Udgivelsesdato: 2009......Bogen: 'En verden af medier – Medialiseringen af politik, sprog, religion og leg' anmeldes. Anmeldelsen kan læses på: http://ojs.statsbiblioteket.dk/index.php/mediekultur/article/view/1370/1499 Udgivelsesdato: 2009...

  16. Forankring af skolemadsordninger

    DEFF Research Database (Denmark)

    Brinck, Nenna Maria; Kristensen, Niels Heine; Hansen, Mette Weinreich

    Denne rapport præsenterer resultaterne af de undersøgelser, der er gennemført i delprojekt 4 i EVIUS evalueringen. Fokus i dette delprojekt er forankringen af skolemadsordningerne. Af undersøgelsen fremgår det, at støtteordningen har virket bedst på skoler, hvor skolelederen har inddraget elever,...

  17. Digital formidling af kulturarv

    DEFF Research Database (Denmark)

    En af tidens store kulturelle dagsordner drejer sig om digital tilgængeliggørelse af kulturarv. Fortidens spor dukker op i nye former på nettet - næsten alle kulturinstitutioner arbejder med, og befolkningen har fået helt nye muligheder for at se med og deltage. Digital formidling af kulturarv...

  18. Ensretning af historieundervisningen

    DEFF Research Database (Denmark)

    Rønhede, Søren

    2008-01-01

    "Det er ikke kun i Putins Rusland, at statsmagten vil dirigere historieundervisningen." (Politiken havde fokuseret på "ensretning af historieundervisningen" i Rusland) "Kanonen fra 2006 er undfanget af Udvalget til Styrkelse af Historie i Folkeskolen. Dette udvalg havde regeringen nedsat; men...

  19. Udformning af en forskningsprotokol

    DEFF Research Database (Denmark)

    Ridder, Hanne Mette Ochsner; Ottesen, Aase Marie

    2008-01-01

    Nogle demenssygdomme rammer især de forreste dele af hjernen, hvilket hos den enkelte person kan medføre svære psykiatriske symptomer. Der findes ingen specifik behandling af grundsygdommene for disse frontotemporaledemenstilstande, og symptomatisk medikamentel behandling af de psykiatriske sympt...

  20. Fremme af Cykeltrafik

    DEFF Research Database (Denmark)

    Madsen, Jens Chr. Overgaard; Silva, Victor; Jensen, Ole B.

    Frem til udgangen af 2013 gennemføres et forskningsprojekt under titlen ”Bikeability”. Formålet med projektet er at fremskaffe dokumenteret viden om, hvordan brugen af cykel bedst muligt kan understøttes. Som en indledende del af projektet er der gennemført en spørgeskemaundersøgelse blandt lande...

  1. Billeder af verdens svovl

    DEFF Research Database (Denmark)

    Samson, Kristine

    2014-01-01

    Film- og billedmediet har en særlig plads i skildringen af vores samfund og økonomi. Produktionen i de vestlige samfund består i dag primært af tegn, symboler, billeder og begær, mens de forgangne tiders maskinelle og, ikke mindst, manuelle bearbejdning af materialer – det vi stadigvæk umiddelbar...

  2. Cell encoding recombinant human erythropoietin

    Energy Technology Data Exchange (ETDEWEB)

    Beck, A.K.; Withy, R.M.; Zabrecky, J.R.; Masiello, N.C.

    1990-09-04

    This patent describes a C127 cell transformed with a recombinant DNA vector. It comprises: a DNA sequence encoding human erythropoietin, the transformed cell being capable of producing N-linked and O-linked glycosylated human erythropoietin.

  3. A novel mutation in the miR-128b gene reduces miRNA processing and leads to glucocorticoid resistance of MLL-AF4 acute lymphocytic leukemia cells.

    Science.gov (United States)

    Kotani, Ai; Ha, Daon; Schotte, Diana; den Boer, Monique L; Armstrong, Scott A; Lodish, Harvey F

    2010-03-15

    MLL-AF4 acute lymphocytic leukemia has a poor prognosis, and the mechanisms by which these leukemias develop are not understood despite intensive research based on well-known concepts and methods. MicroRNAs (miRNAs) are a new class of small noncoding RNAs that post-transcriptionally regulate expression of target mRNA transcripts. We recently reported that ectopic expression of miR-128b together with miR-221, two of the miRNAs downregulated in MLL-AF4 ALL, restores glucocorticoid resistance through downregulation of the MLL-AF4 chimeric fusion proteins MLL-AF4 and AF4-MLL that are generated by chromosomal translocation t(4;11). Here we report the identification of new mutations in miR-128b in RS4;11 cells, derived from MLL-AF4 ALL patient. One novel mutation significantly reduces the processing of miR-128b. Finally, this base change occurs in a primary MLL-AF4 ALL sample as an acquired mutation. These results demonstrate that the novel mutation in miR-128b in MLL-AF4 ALL alters the processing of miR-128b and that the resultant downregulation of mature miR-128b contributes to glucocorticoid resistance through the failure to downregulate the fusion oncogenes.

  4. MicroRNA-205 downregulates mixed-lineage-AF4 oncogene expression in acute lymphoblastic leukemia

    Directory of Open Access Journals (Sweden)

    Dou L

    2013-08-01

    Full Text Available Liping Dou,1,* Jingxin Li,1,* Dehua Zheng,2,* Yonghui Li,1 Xiaoning Gao,1 Chengwang Xu,1 Li Gao,1 Lili Wang,1 Li Yu1 1Department of Hematology, Chinese PLA General Hospital, Beijing, People's Republic of China; 2Department of Hepatobiliary Surgery, Organ Transplant Center, Chinese PLA 309th Hospital, Beijing, People's Republic of China*These authors contributed equally to this workAbstract: Myeloid/lymphoid or mixed-lineage AF4 acute lymphoblastic leukemia (MLL-AF4 ALL is a pediatric leukemia that occurs rarely in adults. MLL-AF4 ALL is typically characterized by the presence of chromosomal translocation (t(4;11(q21;q23, leading to expression of MLL-AF4 fusion protein. Although MLL-AF4 fusion protein triggers a molecular pathogenesis and hematological presentations that are unique to leukemias, the precise role of this oncogene in leukemogenesis remains unclear. Previous studies have indicated that microRNAs (miRs might modulate the expression of MLL-AF4 ALL fusion protein, thereby suggesting the involvement of miR in progression or suppression of MLL-AF4 ALL. We have previously demonstrated that miR-205 negatively regulates transcription of an MLL-AF4 luciferase reporter. Here, we report that exogenous expression of miR-205 in MLL-AF4 human cell lines (RS4;11 and MV4-11 inversely regulates the expression of MLL-AF4 at both messenger RNA (mRNA and protein level. Furthermore, miR-205 significantly induced apoptosis in MLL-AF4 cells as evidenced by Annexin V staining using fluorescence-activated cell sorting (FACS analysis. The proliferative capacity of leukemic cells was suppressed by miR-205. The addition of an miR-205 inhibitor was able to restore the observed effects. In conclusion, these findings demonstrate that miR-205 may have potential value as a novel therapeutic agent in the treatment of MLL-AF4 ALL.Keywords: miR-205, MLL-AF4, leukemia, microRNA, oncogene expression, untranslated regions, proliferation

  5. AFS Monitoring The CERN AFS Console

    CERN Document Server

    CERN. Geneva

    2008-01-01

    CERN's AFS installation serves between 1 and 2 billion accesses per day to its around 20'000 users. Keeping track of the system's overall status and trying to find problems before the users do is not a trivial task, esp. as the installation is growing in almost all aspects. This talk will present CERN's AFS Console, a Lemon- and web-based monitoring tool used by the AFS administrators at CERN to quickly identify problematic entities (servers, partitions, volumes etc.) and to assist them in solving the issues found.

  6. Prekarisering af arbejdsmarkedet

    OpenAIRE

    Sebelin, Randa; Søndergaard Krogh, Niels; Olesen Dørup, Marie; Nielsen, Emil; Westerbye, Nanna

    2005-01-01

    Rapporten redegør for konsekvenserne af fleksibiliseringen af arbejdslivet for den enkelte ansatte. Med et fokus på prekære/løse ansættelser gennemgås først sociologisk teori af bl.a. Ulrich Beck, Oskar Negt og Richard Sennett, som alle fremhæver de negative sider af en løs tilknytning til arbejdsmarkedet. Den danske arbejdsmarkedsmodel og flexicurity-modellen drages ind som baggrundsviden for en vigtig forståelse af den høje tillid til forhandlingsproces frem for lovgivning vi...

  7. EVI1 is critical for the pathogenesis of a subset of MLL-AF9-rearranged AMLs.

    Science.gov (United States)

    Bindels, Eric M J; Havermans, Marije; Lugthart, Sanne; Erpelinck, Claudia; Wocjtowicz, Elizabeth; Krivtsov, Andrei V; Rombouts, Elwin; Armstrong, Scott A; Taskesen, Erdogan; Haanstra, Jurgen R; Beverloo, H Berna; Döhner, Hartmut; Hudson, Wendy A; Kersey, John H; Delwel, Ruud; Kumar, Ashish R

    2012-06-14

    The proto-oncogene EVI1 (ecotropic viral integration site-1), located on chromosome band 3q26, is aberrantly expressed in human acute myeloid leukemia (AML) with 3q26 rearrangements. In the current study, we showed, in a large AML cohort carrying 11q23 translocations, that ∼ 43% of all mixed lineage leukemia (MLL)-rearranged leukemias are EVI1(pos). High EVI1 expression occurs in AMLs expressing the MLL-AF6, -AF9, -AF10, -ENL, or -ELL fusion genes. In addition, we present evidence that EVI1(pos) MLL-rearranged AMLs differ molecularly, morphologically, and immunophenotypically from EVI1(neg) MLL-rearranged leukemias. In mouse bone marrow cells transduced with MLL-AF9, we show that MLL-AF9 fusion protein maintains Evi1 expression on transformation of Evi1(pos) HSCs. MLL-AF9 does not activate Evi1 expression in MLL-AF9-transformed granulocyte macrophage progenitors (GMPs) that were initially Evi1(neg). Moreover, shRNA-mediated knockdown of Evi1 in an Evi1(pos) MLL-AF9 mouse model inhibits leukemia growth both in vitro and in vivo, suggesting that Evi1 provides a growth-promoting signal. Using the Evi1(pos) MLL-AF9 mouse leukemia model, we demonstrate increased sensitivity to chemotherapeutic agents on reduction of Evi1 expression. We conclude that EVI1 is a critical player in tumor growth in a subset of MLL-rearranged AMLs.

  8. Efterforskning af drab i Kosovo

    DEFF Research Database (Denmark)

    Leth, Peter Mygind

    2000-01-01

    Gennemgang af baggrunden for den retsmedicinske mission til Kosovo i 1999, og af 50 obduktioner udført af forfatteren i denne forbindelse.......Gennemgang af baggrunden for den retsmedicinske mission til Kosovo i 1999, og af 50 obduktioner udført af forfatteren i denne forbindelse....

  9. The rate of spontaneous mutations in human myeloid cells

    Energy Technology Data Exchange (ETDEWEB)

    Araten, David J., E-mail: david.araten@nyumc.org [Division of Hematology, Department of Veterans Affairs New York Harbor Healthcare System (United States); Division of Hematology, Department of Medicine, NYU School of Medicine and the NYU Langone Cancer Center (United States); Krejci, Ondrej [Division of Experimental Hematology and Cancer Biology, Cincinnati Children' s Hospital Medical Center, Cincinnati, OH (United States); DiTata, Kimberly [Division of Hematology, Department of Medicine, NYU School of Medicine and the NYU Langone Cancer Center (United States); Wunderlich, Mark [Division of Experimental Hematology and Cancer Biology, Cincinnati Children' s Hospital Medical Center, Cincinnati, OH (United States); Sanders, Katie J.; Zamechek, Leah [Division of Hematology, Department of Medicine, NYU School of Medicine and the NYU Langone Cancer Center (United States); Mulloy, James C. [Division of Experimental Hematology and Cancer Biology, Cincinnati Children' s Hospital Medical Center, Cincinnati, OH (United States)

    2013-09-15

    Highlights: • We provide the first measurement of the mutation rate (μ) in human myeloid cells. • μ is measured to be 3.6–23 × 10{sup −7} per cell division. • The AML-ETO and MLL-AF9 fusions do not seem to increase μ. • Cooperating mutations in NRAS, FLT3 and p53 not seem to increase μ. • Hypermutability may be required to explain leukemogenesis. - Abstract: The mutation rate (μ) is likely to be a key parameter in leukemogenesis, but historically, it has been difficult to measure in humans. The PIG-A gene has some advantages for the detection of spontaneous mutations because it is X-linked, and therefore only one mutation is required to disrupt its function. Furthermore, the PIG-A-null phenotype is readily detected by flow cytometry. Using PIG-A, we have now provided the first in vitro measurement of μ in myeloid cells, using cultures of CD34+ cells that are transduced with either the AML-ETO or the MLL-AF9 fusion genes and expanded with cytokines. For the AML-ETO cultures, the median μ value was ∼9.4 × 10{sup −7} (range ∼3.6–23 × 10{sup −7}) per cell division. In contrast, few spontaneous mutations were observed in the MLL-AF9 cultures. Knockdown of p53 or introduction of mutant NRAS or FLT3 alleles did not have much of an effect on μ. Based on these data, we provide a model to predict whether hypermutability must occur in the process of leukemogenesis.

  10. Characteristics of human amniotic fluid mesenchymal stem cells and their tropism to human ovarian cancer.

    Directory of Open Access Journals (Sweden)

    Liru Li

    Full Text Available The mesenchymal stem cells (MSCs derived from amniotic fluid (AF have become an attractive stem cells source for cell-based therapy because they can be harvested at low cost and avoid ethical disputes. In human research, stem cells derived from AF gradually became a hot research direction for disease treatment, specifically for their plasticity, their reduced immunogenicity and their tumor tropism regardless of the tumor size, location and source. Our work aimed to obtain and characterize human amniotic fluid mesenchymal stem cells (AFMSCs and detect their ovarian cancer tropsim in nude mice model. Ten milliliters of twenty independent amniotic fluid samples were collected from 16-20 week pregnant women who underwent amniocentesis for fetal genetic determination in routine prenatal diagnosis in the first affiliated hospital of Harbin medical university. We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples. AFMSCs presented a fibroblastic-like morphology during the culture. Flow cytometry analyses showed that the cells were positive for specific stem cell markers CD73,CD90, CD105, CD166 and HLA-ABC (MHC class I, but negative for CD 45,CD40, CD34, CD14 and HLA-DR (MHC class II. RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4. AFMSCs could differentiate into bone cells, fat cells and chondrocytes under certain conditions. AFMSCs had the high motility to migrate to ovarian cancer site but didn't have the tumorigenicity. This study enhances the possibility of AFMSCs as drug carrier in human cell-based therapy. Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

  11. Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis

    Directory of Open Access Journals (Sweden)

    Jurate Savickiene

    2015-01-01

    Full Text Available Human amniotic fluid stem cells have become an attractive stem cell source for potential applications in regenerative medicine and tissue engineering. The aim of this study was to characterize amniotic fluid-derived mesenchymal stem cells (AF-MSCs from second- and third-trimester of gestation. Using two-stage protocol, MSCs were successfully cultured and exhibited typical stem cell morphological, specific cell surface, and pluripotency markers characteristics. AF-MSCs differentiated into adipocytes, osteocytes, chondrocytes, myocytes, and neuronal cells, as determined by morphological changes, cell staining, and RT-qPCR showing the tissue-specific gene presence for differentiated cell lineages. Using SYNAPT G2 High Definition Mass Spectrometry technique approach, we performed for the first time the comparative proteomic analysis between undifferentiated AF-MSCs from late trimester of gestation and differentiated into myogenic, adipogenic, osteogenic, and neurogenic lineages. The analysis of the functional and expression patterns of 250 high abundance proteins selected from more than 1400 demonstrated the similar proteome of cultured and differentiated AF-MSCs but the unique changes in their expression profile during cell differentiation that may help the identification of key markers in differentiated cells. Our results provide evidence that human amniotic fluid of second- and third-trimester contains stem cells with multilineage potential and may be attractive source for clinical applications.

  12. Installation af opvaskemaskine

    DEFF Research Database (Denmark)

    Christiansen, J.; Skibstrup Eriksen, S.; Nielsen, F.

    Denne SBI-anvisning er et led i en serie om modernisering af installationerne i den ældre boligmasse. Den henvender sig til både beboere, husejere, VVS-installatører og andre interesserede. Anvisningen indeholder almene afsnit om valg og placering af opvaskemaskine, sagsforløb ved installation......, forhold til myndigheder, priser, finansieringsmuligheder m.m. Anvisningen indeholder endvidere tekniske afsnit om vandinstallation, afløb og elinstallation i forbindelse med installation af opvaskemaskine....

  13. Strategisk ledelse af evalueringskulturen

    Directory of Open Access Journals (Sweden)

    Gitte Orlowitz

    2016-02-01

    Full Text Available Hvis evaluering skal anvendes til at udvikle uddannelser, er en af ledelsen vigtigste opgaver at udvikle en strategi for udvikling af evalueringskulturen – herunder strategier for udvikling af kommunikation og evalueringskapacitet. Den strategiske ledelse kan i den sammenhæng anvendes til at håndtere de ofte modsatte krav, som eksterne og interne interessenter stiller til evaluering.

  14. Administration af informationsmodeller

    DEFF Research Database (Denmark)

    Hundebøl, Jesper

    2013-01-01

    En informationsmodel er i denne sammenhæng en digital repræsentation af et givent byggeri. Både offentlige og private bygherrer anvender informationsmodeller, når der 'bygges digitalt'. I denne artikel undersøges det, hvilke konkrete udfordringer der følger af digitaliseringen, hvor gennemgribend......, klassifikationer og standarder, henvisninger til ydelsesspecifikationer, manualer, paradigmer – endda i form af henvisninger til bilag til bilag....

  15. ARCgame - gamification af undervisning

    DEFF Research Database (Denmark)

    Jensen, Camilla Gyldendahl

    2014-01-01

    Gamification er et begreb, der anvendes til at beskrive brugen af spilelementer i andre miljøer for at forbedre brugernes oplevelse. Følgende tekst har til formål at behandle emnet i forhold til af opnå en forståelse for de elementer der indvirker på en ”gamification af et undervisningsforløb”, her...

  16. Ledelse af Organisationens Kreativitet

    DEFF Research Database (Denmark)

    Hedemann, Lars

    2008-01-01

    forskel, så er man som leder nødt til at arbejde mere målrettet med de medarbejdere som forventes at præstere et kreativt udbytte. Inspireret af analyseværktøjet "KEYS, Assessing the Work Environment for Creativity" udviklet af den amerikanske psykolog og ledelsesforsker ved Harvard University, Teresa...... Amabile, blev en undersøgelse derfor gennemført i udviklingsafdelingerne, Idéland og Idélab hos Bang og Olufsen, for at få et indblik i anvendeligheden af KEYS som inspirationskilde for succesfuld ledelse af organisationens kreativitet....

  17. Analytiske beregninger af induktionsvarme

    DEFF Research Database (Denmark)

    Henningsen, Poul

    Ved opvarmning af et emne ved hjælp af induktion, placeres emnet i en spole. Spolen påføres en vekselstrøm og der induceres en sekundær strøm langs emnets overflade. Dybden af det strømførende lag i emnet afhænger af den på spolens påtrykte strømfrekvens. Ved høje frekvenser løber strømmen i et t...

  18. Bestyrer af egen ro

    DEFF Research Database (Denmark)

    Søndergård, Birger; L. Friis, Klaus

    2000-01-01

    Evnen til at håndtere en indre uro synes at være blevet en væsentlig egenskab i forhold til udviklingen af vort samfund og de hastige brud i kontinuiteten af et liv, der leves. arbejdsforhold. stress. arbejdsmiljø. arbejdsliv......Evnen til at håndtere en indre uro synes at være blevet en væsentlig egenskab i forhold til udviklingen af vort samfund og de hastige brud i kontinuiteten af et liv, der leves. arbejdsforhold. stress. arbejdsmiljø. arbejdsliv...

  19. Konstruktion af transducer

    DEFF Research Database (Denmark)

    Henriksen, Lars; Nielsen, Martin Pram

    Formålet med dette midtvejsprojekt er at udarbejde en transducer til måling af pressers stivhed. Dette er gjort på baggrund af en gennemgang af både presse- og stativ-typer samtidig med at udbøjningssituationen beskrives. Der introduceres en ide, der udgør grundkonceptet for opmålingsproceduren o...... færdige transducer – Load cellen. Strain gauge sørger for dataopsamlingen fra load cellen. Disse kalibreres således at transduceren er klar til de videre målinger der ligger i forlængelse af dette projekt....

  20. The gene for human U2 snRNP auxiliary factor small 35-kDa subunit (U2AF1) maps to the progressive myoclonus epilepsy (EPM1) critical region on chromosome 21q22.3

    Energy Technology Data Exchange (ETDEWEB)

    Lalioti, M.D.; Rossier, C.; Antonarakis, S.E. [Univ. of Geneva Medical School (Switzerland)] [and others

    1996-04-15

    We used targeted exon trapping to clone portions of genes from human chromosome 21q22.3. One trapped sequence showed complete homology with the cDNA of human U2AF{sup 35} (M96982; HGM-approved nomenclature U2AF1), which encodes for the small 35-kDa subunit of the U2 snRNP auxiliary factor. Using the U2AF1 cDNA as a probe, we mapped this gene to cosmid Q15D2, a P1, and YAC 350F7 of the Chumakov et al. contig, close to the cystathionine-{beta}-synthase gene (CBS) on 21q22.3. This localization was confirmed by PCR using oligonucleotides from the 3{prime} UTR and by FISH. As U2AF1 associated with a number of different factors during mRNA splicing, overexpression in trisomy 21 individuals could contribute to some Down syndrome phenotypes by interfering with the splicing process. Furthermore, because this gene maps in the critical region for the progressive myoclonus epilepsy I locus (EPM1), mutation analysis will be carried out in patients to evaluate the potential role of U2AF1 as a candidate for EPM1. 24 refs., 1 fig.

  1. Photobiomodulation on human annulus fibrosus cells during the intervertebral disk degeneration: extracellular matrix-modifying enzymes.

    Science.gov (United States)

    Hwang, Min Ho; Kim, Kyoung Soo; Yoo, Chang Min; Shin, Jae Hee; Nam, Hyo Geun; Jeong, Jin Su; Kim, Joo Han; Lee, Kwang Ho; Choi, Hyuk

    2016-05-01

    Destruction of extracellular matrix (ECM) leads to degeneration of the intervertebral disk (IVD), which is a major contributor to many spine disorders. IVD degeneration is induced by pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), which are secreted by immune cells, including macrophages and neutrophils. The cytokines modulate ECM-modifying enzymes such as matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human annulus fibrosus (AF) cells. The resulting imbalance in catabolic and anabolic enzymes can cause generalized back, neck, and low back pain (LBP). Photobiomodulation (PBM) is known to regulate inflammatory responses and wound healing. The aim of this study was to mimic the degenerative IVD microenvironment, and to investigate the effect of a variety of PBM conditions (wavelength: 635, 525, and 470 nm; energy density: 16, 32, and 64 J/cm(2)) on the production of ECM-modifying-enzymes by AF cells under degenerative conditions induced by macrophage-conditioned medium (MCM), which contains pro-inflammatory cytokines such as TNF-α and IL-β secreted by macrophage during the development of intervertebral disk inflammation. We showed that the MCM-stimulated AF cells express imbalanced ratios of TIMPs (TIMP-1 and TIMP-2) and MMPs (MMP-1 and MMP-3). PBM selectively modulated the production of ECM-modifying enzymes in AF cells. These results suggest that PBM can be a therapeutic tool for degenerative IVD disorders.

  2. Genome engineering in human cells.

    Science.gov (United States)

    Song, Minjung; Kim, Young-Hoon; Kim, Jin-Soo; Kim, Hyongbum

    2014-01-01

    Genome editing in human cells is of great value in research, medicine, and biotechnology. Programmable nucleases including zinc-finger nucleases, transcription activator-like effector nucleases, and RNA-guided engineered nucleases recognize a specific target sequence and make a double-strand break at that site, which can result in gene disruption, gene insertion, gene correction, or chromosomal rearrangements. The target sequence complexities of these programmable nucleases are higher than 3.2 mega base pairs, the size of the haploid human genome. Here, we briefly introduce the structure of the human genome and the characteristics of each programmable nuclease, and review their applications in human cells including pluripotent stem cells. In addition, we discuss various delivery methods for nucleases, programmable nickases, and enrichment of gene-edited human cells, all of which facilitate efficient and precise genome editing in human cells.

  3. Videreudvikling af webbaserede undervisningsprogrammer

    DEFF Research Database (Denmark)

    Blåbjerg, Niels Jørgen

    2005-01-01

    Aalborg Universitetsbiblioteks SWIM projekt blev afsluttet i 2004. I forbindelse med afrapporteringen var der en god anledning til at gøre status over resultater og muligheder for videreudvikling af SWIM konceptet. Perspektiverne i videreudvikling af konceptet var spændende, og det medførte en an...

  4. Konstruktionen af en park

    DEFF Research Database (Denmark)

    Møller, Camilla Hedegaard

    2017-01-01

    Procesorientering er metodeudviklingskrævende Arkitekter rådgiver ikke kun i forhold til den byggede arkitektur i offentlige byrumsprojekter, men også for at opnå særlige processuelle effekter som f.eks. at fremme demokratisk medborgerskab, lokal accept af projekter og løft af hele bykvarterer. P...

  5. Midtvejsevaluering af EGA handlingsplanen

    DEFF Research Database (Denmark)

    Møller, Niels; Hasle, Peter; Christiansen, Jørgen Møller

    Center for Alternativ Samfundsanalyse (CASA) og Institut for Teknologi og Samfund ved Danmarks Tekniske Universitet har gennemført denne midtvejsevaluering af arbejdsmarkedets parters handlingsplan mod reduktion af ensidigt gentaget arbejde. Undersøgelsen viser, at der er sket en betydelig...

  6. Dialektisk billede af Benjamin

    DEFF Research Database (Denmark)

    Bøggild, Jacob

    2004-01-01

    Anmeldelse af Lars Brückner: "Løse blade. Læseren, kritikeren og fortælleren Walter Benjamin"......Anmeldelse af Lars Brückner: "Løse blade. Læseren, kritikeren og fortælleren Walter Benjamin"...

  7. Kvalitetsvurdering af sundhedsoplysende materiale

    DEFF Research Database (Denmark)

    Osler, Merete; Bech, Lars; Sybille, Bojlén

    1990-01-01

    Lægeforeningens Hygiejnekomités arbejdsgruppe vedrørende sundhedsoplysning har udarbejdet en "huskeliste" til brug for en kvalitetsvurdering af skriftligt sundhedsoplysende materiale......Lægeforeningens Hygiejnekomités arbejdsgruppe vedrørende sundhedsoplysning har udarbejdet en "huskeliste" til brug for en kvalitetsvurdering af skriftligt sundhedsoplysende materiale...

  8. Ud af guldrammerne

    DEFF Research Database (Denmark)

    Munch, Anders V.

    2006-01-01

    Diskussion af den internationale indflydelse på dansk symbolisme og skønvirke med udgangspunkt i Johan Rohdes rejsejournaler og Emil Hannovers anmeldelser af internationale udstillinger og udgivelser. Særligt fokus er Gesamtkunstwerk-tanken som baggrund for at koble kunst og kunsthåndværk....

  9. Midtvejsevaluering af EGA handlingsplanen

    DEFF Research Database (Denmark)

    Møller, Niels; Hasle, Peter; Christiansen, Jørgen Møller

    reduktion af det ensidige gentage arbejde i den første del af handlingsplanens periode i størrelsesordenen 25%. Mange virksomheder har foretaget en omfattende indsats overfor arbejdsmiljøproblemet, men der er også virksomheder som intet har gjort. I mange tilfælde er virksomhedernes indsats kvalitetsmæssig...

  10. Kuratering af Samtidskunst

    DEFF Research Database (Denmark)

    Kuratering af Samtidskunst er den første bogudgivelse i Danmark, der fokuserer på kuratering som fag og praksis. Kuratering spiller en stadig større rolle i samtidskunsten. Bogen er en introduktion til skiftende forståelser af udstillings- og kuratorpraksisser og deres udfordringer, indkredset ge...

  11. Raman af hvide pigmenter

    DEFF Research Database (Denmark)

    Reeler, Nini Elisabeth Abildgaard; Nielsen, Ole Faurskov; Sauer, Stephan P. A.;

    2013-01-01

    Et samspil mellem kunst og kemi. I et samarbejde mellem Statens Museum for Kunst og Kemisk Institut på KU er Ramanspek-troskopi brugt til at definere sammensætningen af blandinger af blyhvidt og calcit i maleriers hvide pigmenter....

  12. Evaluering af VIFT

    DEFF Research Database (Denmark)

    Pedersen, Michael Svendsen

    Denne rapport indeholder en ekstern evaluering af aktiviteter i VIFT, Videncenter for bedre undervisning af tosprogede elever, der er etableret som et samarbejde mellem Videncenter for tosprogethed & interkulturalitet (UC2) ved Professionshøjskolen København (UCC), tidligere CVU København...

  13. Coaching af dit studieliv

    DEFF Research Database (Denmark)

    Hansen, Rasmus Thorning

    2008-01-01

    En generel beskrivelse af de problemer specialestuderende sidder med og hvorledes coaching kan hjælpe med at (gen)skabe motivation og fokus......En generel beskrivelse af de problemer specialestuderende sidder med og hvorledes coaching kan hjælpe med at (gen)skabe motivation og fokus...

  14. Anvendelse af genmodificerede planter

    DEFF Research Database (Denmark)

    Møller, F.

    I rapporten vurderes mulighederne for at gennemføre velfærdsøkonomiske analyser af anvendelsen af genmodificerede planter. Der gøres rede for kravene til konse-kvensbeskrivelsen og for mulighederne for at prissætte såvel de markedsrelaterede som de ikke-markedsrelaterede konsekvenser. Mulighederne...

  15. Behandlingseffekt af Digital Dermatitis

    DEFF Research Database (Denmark)

    Krogh, Kenneth; Thomsen, Peter

    2008-01-01

    af klovlidelser herunder især Digital Dermatitis. Klovregistreringerne viser, at der er stor dynamik og mange nyinfektioner af Digital Dermatitis svarende til problematikken ved mastitis. Behandlingseffekten ved Digital Dermatitis er høj (omkring 90 %) ved den udførte behandling. Behandlingen bestod...

  16. Behandlingseffekt af Digital Dermatitis

    DEFF Research Database (Denmark)

    Krogh, Kenneth; Thomsen, Peter

    2008-01-01

    af klovlidelser herunder især Digital Dermatitis. Klovregistreringerne viser, at der er stor dynamik og mange nyinfektioner af Digital Dermatitis svarende til problematikken ved mastitis. Behandlingseffekten ved Digital Dermatitis er høj (omkring 90 %) ved den udførte behandling. Behandlingen bestod...

  17. Kvalitetssikring af pressvejseprocessen

    DEFF Research Database (Denmark)

    Rasmussen, Mogens H.

    Det oprindelige formål med dette arbejde var udvikling af procesovervågning til pressvejsning ved en udvalgt hovedgeometri. Hovedgeometrien, ring/rør geometrien i materialekombinationerne stålring til tinbronzerør, blev valgt efter en forundersøgelse. På baggrund af et litteraturstudie samt indle...

  18. Anvendelse af genmodificerede planter

    DEFF Research Database (Denmark)

    Møller, F.

    I rapporten vurderes mulighederne for at gennemføre velfærdsøkonomiske analyser af anvendelsen af genmodificerede planter. Der gøres rede for kravene til konse-kvensbeskrivelsen og for mulighederne for at prissætte såvel de markedsrelaterede som de ikke-markedsrelaterede konsekvenser. Mulighederne...

  19. Indsattes organisering af selvforplejning

    DEFF Research Database (Denmark)

    Kjær Minke, Linda

    2012-01-01

    organiserer sig i madgrupper. Dette dels af økonomiske og praktiske hensyn, men madgrupperne fungerer også som mulighedsrum til fremvisning af forskellige former for kapital, positionering og magt. Madgrupperne har derfor både økonomiske og sociale formål og har betydning for indsattes strategier i...

  20. Raman af hvide pigmenter

    DEFF Research Database (Denmark)

    Reeler, Nini Elisabeth Abildgaard; Nielsen, Ole Faurskov; Sauer, Stephan P. A.

    2013-01-01

    Et samspil mellem kunst og kemi. I et samarbejde mellem Statens Museum for Kunst og Kemisk Institut på KU er Ramanspek-troskopi brugt til at definere sammensætningen af blandinger af blyhvidt og calcit i maleriers hvide pigmenter....

  1. Implementering af evidensbaseret praksis

    DEFF Research Database (Denmark)

    Scheel, Linda Schumann; Tewes, Marianne; Petersen, Preben Ulrich

    2015-01-01

    Projektet omhandler implementering af den kliniske retningslinje om identifikation, forebyggelse og behandling af delirium – i denne sammenhæng relateret til hjertepatienter. Projektet foregår på to hospitaler i Hjertecentret på Rigshospitalet, et intensivt afsnit og et sengeafsnit, i tæt...

  2. Implementering af evidensbaseret praksis

    DEFF Research Database (Denmark)

    Scheel, Linda Schumann; Hansen, Ida Rode

    2014-01-01

    Projektet omhandler implementering af den kliniske retningslinje om identifikation, forebyggelse og behandling af delirium – i denne sammenhæng relateret til hjertepatienter. Projektet foregår på to hospitaler i Hjertecentret på Rigshospitalet, et intensivt afsnit og et sengeafsnit, i tæt...

  3. Evaluering af afdeling H

    DEFF Research Database (Denmark)

    Kjær Minke, Linda; Birkmose, Sofie Meldal

    2016-01-01

    Som led i Kriminalforsorgens flerårsaftale 2013-2016 blev det åbne statsfængsel Søbysøgård i januar 2015 udvidet med 42 lukkede pladser i den nybyggede afdeling H. Nærværende evaluering kortlægger de erfaringer personalet og indsatte har gjort sig i løbet af det første år. Etableringen af den...... lukkede fængselsafdeling H giver en unik mulighed for at følge henholdsvis hvilke faktorer, der bidrager til udvikling af fængselskultur, og hvordan de respektive faktorer influerer på udvikling af fængselskulturen. Evalueringens metoder består af kvalitative og kvantitative metoder såsom dokumentanalyse...

  4. Hypoxic Conditioned Medium from Human Amniotic Fluid-Derived Mesenchymal Stem Cells Accelerates Skin Wound Healing through TGF-β/SMAD2 and PI3K/Akt Pathways

    Directory of Open Access Journals (Sweden)

    Eun Kyoung Jun

    2014-01-01

    Full Text Available In a previous study, we isolated human amniotic fluid (AF-derived mesenchymal stem cells (AF-MSCs and utilized normoxic conditioned medium (AF-MSC-norCM which has been shown to accelerate cutaneous wound healing. Because hypoxia enhances the wound healing function of mesenchymal stem cell-conditioned medium (MSC-CM, it is interesting to explore the mechanism responsible for the enhancement of wound healing function. In this work, hypoxia not only increased the proliferation of AF-MSCs but also maintained their constitutive characteristics (surface marker expression and differentiation potentials. Notably, more paracrine factors, VEGF and TGF-β1, were secreted into hypoxic conditioned medium from AF-MSCs (AF-MSC-hypoCM compared to AF-MSC-norCM. Moreover, AF-MSC-hypoCM enhanced the proliferation and migration of human dermal fibroblasts in vitro, and wound closure in a skin injury model, as compared to AF-MSC-norCM. However, the enhancement of migration of fibroblasts accelerated by AF-MSC-hypoCM was inhibited by SB505124 and LY294002, inhibitors of TGF-β/SMAD2 and PI3K/AKT, suggesting that AF-MSC-hypoCM-enhanced wound healing is mediated by the activation of TGF-β/SMAD2 and PI3K/AKT. Therefore, AF-MSC-hypoCM enhances wound healing through the increase of hypoxia-induced paracrine factors via activation of TGF-β/SMAD2 and PI3K/AKT pathways.

  5. Smagen af svovl

    DEFF Research Database (Denmark)

    Madsen, P. V.; Sørensen, B. T.

    2007-01-01

    Et af Sydafrikas største industriområder ligger i landets tredje største by, Durban. Industriområdet kaldes for "South Durban Industrial Basin" (SDIB), hvor der er cirka 120.000 beboere. Boligerne ligger side om side med en bred variation af tung industri. Området er et sørgeligt eksempel på den ...... tidligere Apartheid styret byplanlægning, hvor regeringen i 1940'erne valgte at placere industri i et område hovedsageligt beboet af farvede og sorte mennesker....

  6. Revisiting the biology of infant t(4;11)/MLL-AF4+ B-cell acute lymphoblastic leukemia

    Science.gov (United States)

    Bueno, Clara; Prieto, Cristina; Acha, Pamela; Stam, Ronald W.; Marschalek, Rolf; Menéndez, Pablo

    2015-01-01

    Infant B-cell acute lymphoblastic leukemia (B-ALL) accounts for 10% of childhood ALL. The genetic hallmark of most infant B-ALL is chromosomal rearrangements of the mixed-lineage leukemia (MLL) gene. Despite improvement in the clinical management and survival (∼85-90%) of childhood B-ALL, the outcome of infants with MLL-rearranged (MLL-r) B-ALL remains dismal, with overall survival leukemia latency. Despite its aggressiveness and short latency, current progress on its etiology, pathogenesis, and cellular origin is limited as evidenced by the lack of mouse/human models recapitulating the disease phenotype/latency. We propose this is because infant cancer is from an etiologic and pathogenesis standpoint distinct from adult cancer and should be seen as a developmental disease. This is supported by whole-genome sequencing studies suggesting that opposite to the view of cancer as a “multiple-and-sequential-hit” model, t(4;11) alone might be sufficient to spawn leukemia. The stable genome of these patients suggests that, in infant developmental cancer, one “big-hit” might be sufficient for overt disease and supports a key contribution of epigenetics and a prenatal cell of origin during a critical developmental window of stem cell vulnerability in the leukemia pathogenesis. Here, we revisit the biology of t(4;11)+ infant B-ALL with an emphasis on its origin, genetics, and disease models. PMID:26463423

  7. Sammenligning af kvaliteten af kommunernes sagsbehandling

    DEFF Research Database (Denmark)

    Rasmussen, Martin

    flere forskellige spørgsmål fra praksis¬undersøgelser i samme sammenligning. Vi foreslår også, at metoder til såkaldte ’multi¬ple sammenligninger’ bruges til dele af undersøgelserne. Det er en ældre og ikke særlig kendt metode, men den er særdeles relevant både i den konkrete anvendelse og i andre...

  8. Test af Software

    DEFF Research Database (Denmark)

    Dette dokument udgør slutrapporten for netværkssamarbejdet ”Testnet”, som er udført i perioden 1.4.2006 til 31.12.2008. Netværket beskæftiger sig navnlig med emner inden for test af indlejret og teknisk software, men et antal eksempler på problemstillinger og løsninger forbundet med test af...... administrativ software indgår også. Rapporten er opdelt i følgende 3 dele: Overblik. Her giver vi et resumé af netværkets formål, aktiviteter og resultater. State of the art af software test ridses op. Vi omtaler, at CISS og netværket tager nye tiltag. Netværket. Formål, deltagere og behandlede emner på ti...... række danske software-, elektronik- og IT-virksomheder....

  9. Test af Software

    DEFF Research Database (Denmark)

    Dette dokument udgør slutrapporten for netværkssamarbejdet ”Testnet”, som er udført i perioden 1.4.2006 til 31.12.2008. Netværket beskæftiger sig navnlig med emner inden for test af indlejret og teknisk software, men et antal eksempler på problemstillinger og løsninger forbundet med test af...... administrativ software indgår også. Rapporten er opdelt i følgende 3 dele: Overblik. Her giver vi et resumé af netværkets formål, aktiviteter og resultater. State of the art af software test ridses op. Vi omtaler, at CISS og netværket tager nye tiltag. Netværket. Formål, deltagere og behandlede emner på ti...... række danske software-, elektronik- og IT-virksomheder....

  10. Supervision af psykoterapi

    DEFF Research Database (Denmark)

    SUPERVISION AF PSYKOTERAPI indtager en central position i uddannelsen og udviklingen af psykoterapeuter. Trods flere lighedspunkter med psykoterapi, undervisning og konsultation er psykoterapisupervision et selvstændigt virksomhedsområde. Supervisor må foruden at være en trænet psykoterapeut kende...... supervisionens rammer og indplacering i forhold til organisation og samfund. En række kapitler drejer sig om supervisors opgaver, roller og kontrolfunktion, supervision set fra supervisandens perspektiv samt betragtninger over relationer og processer i supervision. Der drøftes fordele og ulemper ved de...... forskellige måder, hvorpå en sag kan fremlægges. Bogens første del afsluttes med refleksioner over de etiske aspekter ved psykoterapisupervision. Bogens anden del handler om de særlige forhold, der gør sig gældende ved supervision af en række specialiserede behandlingsformer eller af psykoterapi med bestemte...

  11. Termisk forgasning af biomasse

    DEFF Research Database (Denmark)

    Indlæggene i referatet er gengivet efter båndoptagelser. Der er dog foretaget nogle enklte omskrivninger af hensyn til læseligheden. Foredragsholdernes overheads er endvidere blevet lagt ind i referatet....

  12. Vitalisering af rettetheder

    DEFF Research Database (Denmark)

    Kristensen, René

    2012-01-01

    Kapitlet handler om at anvende det selvpsykologiske teorigrundlag i samspil med de systemisk konstruktionistiske ideer i arbejdet med at udvikle organisationer og samtidig kvalificere anvendelsen af PUMA -pædagogisk udredning i inklusionssammenhænge.......Kapitlet handler om at anvende det selvpsykologiske teorigrundlag i samspil med de systemisk konstruktionistiske ideer i arbejdet med at udvikle organisationer og samtidig kvalificere anvendelsen af PUMA -pædagogisk udredning i inklusionssammenhænge....

  13. Forebyggelse af kriminalitet

    DEFF Research Database (Denmark)

    Christiansen, U.; Jantzen, E.B.; Kirkegaard, O.

    Forebyggelse af kriminalitet i boligområder har længe stået på dagsordenen i alle vestlige samfund. Dansk Ingeniørforening og Ingeniørsammenslutningen har udviklet normer for kriminalpræventiv planlægning i boligområder siden midten af 1980'erne. To bebyggelser i Københavnsegnen er blevet planlag...

  14. Af Upraktiske Grunde

    DEFF Research Database (Denmark)

    Vaaben, Nana Katrine

    2001-01-01

    Artiklen baseres på studier af danske musikfans og handler om de objekter, de indsamler i forbindelse med at være fans. Der er to hovedargumenter, der løber gennem hele analysen. For det første at det indsamling af objekter handler om, er begær efter at kategorisere, ordne og systematisere, såled...

  15. Bearbejdning af Luhmanns organisationsteori

    DEFF Research Database (Denmark)

    Mortensen, Nils

    Som titlen siger, er det en gennemgang/præsertation af Luhmanns organisationsteori. Der er lagt særlig vægt på emnerne beslutning, ledelse opg forandring......Som titlen siger, er det en gennemgang/præsertation af Luhmanns organisationsteori. Der er lagt særlig vægt på emnerne beslutning, ledelse opg forandring...

  16. Diffusion inside living human cells

    DEFF Research Database (Denmark)

    Leijnse, N.; Jeon, J. -H.; Loft, Steffen

    2012-01-01

    Naturally occurring lipid granules diffuse in the cytoplasm and can be used as tracers to map out the viscoelastic landscape inside living cells. Using optical trapping and single particle tracking we found that lipid granules exhibit anomalous diffusion inside human umbilical vein endothelial...... cells. For these cells the exact diffusional pattern of a particular granule depends on the physiological state of the cell and on the localization of the granule within the cytoplasm. Granules located close to the actin rich periphery of the cell move less than those located towards to the center...... of the cell or within the nucleus. Also, granules in cells which are stressed by intense laser illumination or which have attached to a surface for a long period of time move in a more restricted fashion than those within healthy cells. For granules diffusing in healthy cells, in regions away from the cell...

  17. Tuberin and PRAS40 are anti-apoptotic gatekeepers during early human amniotic fluid stem-cell differentiation.

    Science.gov (United States)

    Fuchs, Christiane; Rosner, Margit; Dolznig, Helmut; Mikula, Mario; Kramer, Nina; Hengstschläger, Markus

    2012-03-01

    Embryoid bodies (EBs) are three-dimensional multicellular aggregates allowing the in vitro investigation of stem-cell differentiation processes mimicking early embryogenesis. Human amniotic fluid stem (AFS) cells harbor high proliferation potential, do not raise the ethical issues of embryonic stem cells, have a lower risk for tumor development, do not need exogenic induction of pluripotency and are chromosomal stable. Starting from a single human AFS cell, EBs can be formed accompanied by the differentiation into cells of all three embryonic germ layers. Here, we report that siRNA-mediated knockdown of the endogenous tuberous sclerosis complex-2 (TSC2) gene product tuberin or of proline-rich Akt substrate of 40 kDa (PRAS40), the two major negative regulators of mammalian target of rapamycin (mTOR), leads to massive apoptotic cell death during EB development of human AFS cells without affecting the endodermal, mesodermal and ectodermal cell differentiation spectrum. Co-knockdown of endogenous mTOR demonstrated these effects to be mTOR-dependent. Our findings prove this enzyme cascade to be an essential anti-apoptotic gatekeeper of stem-cell differentiation during EB formation. These data allow new insights into the regulation of early stem-cell maintenance and differentiation and identify a new role of the tumor suppressor tuberin and the oncogenic protein PRAS40 with the relevance for a more detailed understanding of the pathogenesis of diseases associated with altered activities of these gene products.

  18. Human stromal (mesenchymal) stem cells

    DEFF Research Database (Denmark)

    Aldahmash, Abdullah; Zaher, Walid; Al-Nbaheen, May

    2012-01-01

    Human stromal (mesenchymal) stem cells (hMSC) represent a group of non-hematopoietic stem cells present in the bone marrow stroma and the stroma of other organs including subcutaneous adipose tissue, placenta, and muscles. They exhibit the characteristics of somatic stem cells of self-renewal and......Human stromal (mesenchymal) stem cells (hMSC) represent a group of non-hematopoietic stem cells present in the bone marrow stroma and the stroma of other organs including subcutaneous adipose tissue, placenta, and muscles. They exhibit the characteristics of somatic stem cells of self...... of clinical applications, e.g., non-healing bone fractures and defects and also non-skeletal degenerative diseases like heart failure. Currently, the numbers of clinical trials that employ MSC are increasing. However, several biological and biotechnological challenges need to be overcome to benefit from...

  19. Immunterapi af cancersygdomme

    DEFF Research Database (Denmark)

    Andersen, Mads Hald; Schmidt, Henrik; Gehl, Julie

    2002-01-01

    with various sources of antigen and cytokines for therapeutic vaccinations against cancer. The present paper summarizes current knowledge in the field of tumour immunology, and reviews various approaches undertaken with the aim of inducing strong anti-tumour responses. To this end, we review data from clinical......Significant advances in the understanding of the cells and molecules involved in tumour/host interactions in humans have been made over the last decades. Thus, through studies of the interactions between cells of the immune system and cancer cells, several tumour antigens and their epitopes...... recognised by human leucocyte antigen class I-restricted cytotoxic T lymphocytes have been identified. Likewise, specialised antigen presenting cells have been characterised and methods developed that allow the use of such cells in clinical immunology. In the clinic, systemic administration of immune...

  20. Realisering af Vision 2020

    DEFF Research Database (Denmark)

    Bertelsen, Niels Haldor; Hansen, Ernst Jan de Place

    Repræsentanter for byggesektoren har på 11 dialogmøder drøftet Erhvervs- og Byggestyrelsens "Vision 2020 - Byggeri med mening". Drøftelserne førte til formulering af en lang række initiativforslag til realisering af visionen. Den mest centrale udfordring bliver at reducere fejl og mangler i...... byggeriet. Branchen lægger også vægt på, at styringen af Vision 2020s reaisering sker i byggesektoren. Initiativforslagene er i rapporten samlet under 3 hovedområder. Det første hovedområde lægger vægt på bygningerne, brugerbehov og det globale samfund. Det andet omhandler processen og leverancesystemet...

  1. Anvendeligheden af GIS

    DEFF Research Database (Denmark)

    Winstrup, Mie; Levin, Gregor

    2011-01-01

    Fragmentering af naturen er en trussel mod biodiversiteten, og etablering af økologiske forbindelser/korridorer mellem naturområderne er én måde hvorved nedgangen i biodiversitet kan stoppes. Med Næstved Kommune som case-område har jeg undersøgt, hvordan analyser i GIS kan bruges til at udvælge...... omdannet til reelle korridorer, hvor bredden afhænger af arealdækket som forbindelserne krydser. I implementerings øjemed er det anvendeligt at vide om nogle forbindelser er særlig vigtige for at skabe mere sammenhængende natur. GIS er anvendeligt hertil, idet GIS kan bruges til at bestemme den enkelte...

  2. Anvendeligheden af GIS

    DEFF Research Database (Denmark)

    Winstrup, Mie; Levin, Gregor

    2011-01-01

    Fragmentering af naturen er en trussel mod biodiversiteten, og etablering af økologiske forbindelser/korridorer mellem naturområderne er én måde hvorved nedgangen i biodiversitet kan stoppes. Med Næstved Kommune som case-område har jeg undersøgt, hvordan analyser i GIS kan bruges til at udvælge...... omdannet til reelle korridorer, hvor bredden afhænger af arealdækket som forbindelserne krydser. I implementerings øjemed er det anvendeligt at vide om nogle forbindelser er særlig vigtige for at skabe mere sammenhængende natur. GIS er anvendeligt hertil, idet GIS kan bruges til at bestemme den enkelte...

  3. Ledelse af sociale institutioner

    DEFF Research Database (Denmark)

    Kofod, Kasper

    Ledelse af sociale institutioner giver et overblik over den enorme forandring, der er sket indenfor det sociale område i de sidste 25-30 år. Moderniseringen af den offentlige sektor har været gennemgribende, og udviklingen på det sociale område er gået mod større instirutioner og mod, at stadig...... flere beslutninger træffes lokalt. det betyder også,a t der kommeret øget fokus på ledelse af de enkelte sociale institutioner. Forandringer i omverdenedn som globalisering, individualisering, mangel på arbejdskraft og ændringer i omgangsformer påvirker også ledelsesvilkårene i de sociale institutioner...

  4. Cloned, CD117 selected human amniotic fluid stem cells are capable of modulating the immune response.

    Directory of Open Access Journals (Sweden)

    Emily C Moorefield

    Full Text Available Amniotic fluid stem (AFS cells are broadly multipotent, can be expanded extensively in culture, are not tumorigenic and can be readily cryopreserved for cell banking. Mesenchymal stem cells (MSC show immunomodulatory activity and secrete a wide spectrum of cytokines and chemokines that suppress inflammatory responses, block mixed lymphocyte reactions (MLR and other immune reactions, and have proven therapeutic against conditions such as graft-versus-host disease. AFS cells resemble MSCs in many respects including surface marker expression and differentiation potential. We therefore hypothesized that AFS cells may exhibit similar immunomodulatory capabilities. We present data to demonstrate that direct contact with AFS cells inhibits lymphocyte activation. In addition, we show that cell-free supernatants derived from AFS cells primed with total blood monocytes or IL-1β, a cytokine released by monocytes and essential in mediation of the inflammatory response, also inhibited lymphocyte activation. Further investigation of AFS cell-free supernatants by protein array revealed secretion of multiple factors in common with MSCs that are known to be involved in immune regulation including growth related oncogene (GRO and monocyte chemotactic protein (MCP family members as well as interleukin-6 (IL-6. AFS cells activated by PBMCs released several additional cytokines as compared to BM-MSCs, including macrophage inflammatory protein-3α (MIP-3α, MIP-1α and Activin. AFS cells also released higher levels of MCP-1 and lower levels of MCP-2 compared to BM-MSCs in response to IL-1β activation. This suggests that there may be some AFS-specific mechanisms of inhibition of lymphocyte activation. Our results indicate that AFS cells are able to suppress inflammatory responses in vitro and that soluble factors are an essential component in the communication between lymphocytes and AFS cells. Their extensive self-renewal capacity, possibility for banking and

  5. Konstruktion af sandheden

    DEFF Research Database (Denmark)

    Nørreklit, Lennart

    2009-01-01

    Artiklen fremstiller sandhedsbegrebet som et læringsideal, som erstatter absolutisme og relativisme med læring. Indledningen bestemmer en problemorienteret ramme, som teorien indgår i: Mennesket er aktør, der danner virkelighed i form af relationer til sin omverden. Sandhedsbegrebet er forudsætni...... for en løbende sandhedsbestemmende læringsproces. Artiklen slutter med en diskussion af performative problemer, hvorigennem udsagn kan påvirke deres egen sandhedsværdi....

  6. Opbygning af laboratoriefaciliteter til induktionsopvarmning

    DEFF Research Database (Denmark)

    Henningsen, Poul

    Denne rapport beskriver de faciliteter, der er opbygget til induktionsopvarmning i IPL’s svejselaboratorium. Beskrivelsen omfatter: • Indkøb og installation af frekvensgenerator • Fabrikation af spoler • Positionering og fastholdelse af emner og spole • Måling og registrering.......Denne rapport beskriver de faciliteter, der er opbygget til induktionsopvarmning i IPL’s svejselaboratorium. Beskrivelsen omfatter: • Indkøb og installation af frekvensgenerator • Fabrikation af spoler • Positionering og fastholdelse af emner og spole • Måling og registrering....

  7. EVI1 is critical for the pathogenesis of a subset of MLL-AF9–rearranged AMLs

    Science.gov (United States)

    Bindels, Eric M. J.; Havermans, Marije; Lugthart, Sanne; Erpelinck, Claudia; Wocjtowicz, Elizabeth; Krivtsov, Andrei V.; Rombouts, Elwin; Armstrong, Scott A.; Taskesen, Erdogan; Haanstra, Jurgen R.; Beverloo, H. Berna; Döhner, Hartmut; Hudson, Wendy A.; Kersey, John H.; Kumar, Ashish R.

    2012-01-01

    The proto-oncogene EVI1 (ecotropic viral integration site-1), located on chromosome band 3q26, is aberrantly expressed in human acute myeloid leukemia (AML) with 3q26 rearrangements. In the current study, we showed, in a large AML cohort carrying 11q23 translocations, that ∼ 43% of all mixed lineage leukemia (MLL)–rearranged leukemias are EVI1pos. High EVI1 expression occurs in AMLs expressing the MLL-AF6, -AF9, -AF10, -ENL, or -ELL fusion genes. In addition, we present evidence that EVI1pos MLL-rearranged AMLs differ molecularly, morphologically, and immunophenotypically from EVI1neg MLL-rearranged leukemias. In mouse bone marrow cells transduced with MLL-AF9, we show that MLL-AF9 fusion protein maintains Evi1 expression on transformation of Evi1pos HSCs. MLL-AF9 does not activate Evi1 expression in MLL-AF9–transformed granulocyte macrophage progenitors (GMPs) that were initially Evi1neg. Moreover, shRNA-mediated knockdown of Evi1 in an Evi1pos MLL-AF9 mouse model inhibits leukemia growth both in vitro and in vivo, suggesting that Evi1 provides a growth-promoting signal. Using the Evi1pos MLL-AF9 mouse leukemia model, we demonstrate increased sensitivity to chemotherapeutic agents on reduction of Evi1 expression. We conclude that EVI1 is a critical player in tumor growth in a subset of MLL-rearranged AMLs. PMID:22553314

  8. Human embryonic stem cells handbook

    Directory of Open Access Journals (Sweden)

    Carlo Alberto Redi

    2013-03-01

    Full Text Available After the Nobel prize in physiology or medicine was awarded jointly to Sir John Gurdon and Shinya Yamanaka for the discovery that mature cells can be reprogrammed to become pluripotent it became imperative to write down the review for a book entirely devoted to human embryonic stem cells (hES, those cells that are a urgent need for researchers, those cells that rekindle the ethical debates and finally, last but not least, those cells whose study paved the way to obtain induced pluripotent stem cells by the OSKC’s Yamanaka method (the OSKC acronim refers, for those not familiar with the topic, to the four stemness genes used to transfect somatic fibroblasts: Oct4, Sox2, Klf4 and c-Myc....

  9. Forplumring af historien

    DEFF Research Database (Denmark)

    Winther, Gorm

    2006-01-01

    Umiddelbart efer invasionen og samme år i 1956 fik Larsenfløjen udvirket en udtalelse fra DKPs forretningsudvalg, hvor det knæsattes at ethvert folk selvstændigt skulle kunne bestemme sin udviklingsvej, ligesom man fordømte påkaldelsen af hjælp fra broderpartierne udefra....

  10. Innovationen lider af teknologifetichisme

    DEFF Research Database (Denmark)

    Pedersen, Peder Holm

    2011-01-01

    Teknologisk innovation er udråbt som vejen til fremtiden for den danske velfærdsstat. Men vi risikerer at miste innovationens fulde potentiale, hvis vi tror, at innovation nødvendigvis skal lugte af teknologi. Nogle gange kan en sort affaldssæk også gøre det....

  11. Definition af et landdistrikt

    DEFF Research Database (Denmark)

    Søgaard, Villy

    2008-01-01

    Forskellige lande arbejder med meget forskellige definitioner af begrebet landdistrikt. Arbejdspapiret diskuterer først en række generelle krav til definitioner og anbefaler på den baggrund konkrete justeringer i forhold til den hidtidige danske praksis på området. Arbejdspapiret er blevet til i...

  12. Forankring af skolemadsordninger

    DEFF Research Database (Denmark)

    Brinck, Nenna Maria; Kristensen, Niels Heine; Hansen, Mette Weinreich

    I rapporten beskrives tre forældrebetalte skolemadsordninger, der blev etableret med støtte fra FødevareErhverv i 2008. Rapporten indeholder også resultater fra en spørgeskemaundersøgelse foretaget blandt elever og forældre i en 4. og 8. klasse på de tre caseskoler. Rapporten er en del af evaluer...

  13. Inklusion af utilpassede unge

    DEFF Research Database (Denmark)

    2015-01-01

    Anholt-projektet repræsenterer en alternativ pædagogisk interventionsform for utilpassede og sårbare unge. Projektet, der i en årrække har været afprøvet af ungdomsskoler og ungdomsorganisationer i Danmark, Tyskland, Østrig, Spanien, Portugal og Italien, bygger på tanker om erfaringsbaseret, ufor...

  14. En skygge af modernitet

    DEFF Research Database (Denmark)

    2015-01-01

    Artiklen vil kort skitsere forskellige, samtidige konservative reaktioner på teknikken, hvis destruktive potentiale var blevet åbenbart i og med Første Verdenskrig, og derefter gennem en læsning af Drachmanns politiske og skønlitterære forfatterskab identificere nøgleelementer og begreber i Drach...

  15. Indeklimavurdering af linolieholdige byggematerialer

    DEFF Research Database (Denmark)

    Knudsen, Henrik Nellemose; Clausen, P.A.; Shibuya, H.

    Rapporten gennemgår en undersøgelse af afgasningen fra tre typer linolieholdige byggematerialer (gulvbelægning, vægmaling og gulvolie) og tilsvarende konventionelle ikke-linolieholdige syntetiske materialer. Undersøgelsen viser, at de undersøgte linolieholdige materialer forværrede den oplevede l...

  16. Efterisolering af etageboliger

    DEFF Research Database (Denmark)

    Munch-Andersen, Jørgen

    Denne anvisning behandler efterisolering af etageboliger bygget i perioden 1850-1977. Der gøres rede for de grundlæggende principper for efterisolering og de bygningsfysiske krav til de enkelte bygningsdele. Anvisningen indeholder desuden vejledning i, hvordan efterisolering udføres i henholdsvis...

  17. Anmeldelse af: Gruppepsykologi

    DEFF Research Database (Denmark)

    Christensen, Bodil

    2009-01-01

    , kreativitet og nytænkning. Det er her, videnskabens virkelige fremskridt sker.” Sådan lyder et citat af Albert Einstein, som Lars Svedberg bruger for at sætte sin faglige fremstilling om gruppepsykologi i perspektiv. Gruppeorganisationen er nemlig ofte rum for/ eller kan være rum for denne synergieffekt, der...

  18. Definition af et landdistrikt

    DEFF Research Database (Denmark)

    Søgaard, Villy

    2008-01-01

    Forskellige lande arbejder med meget forskellige definitioner af begrebet landdistrikt. Arbejdspapiret diskuterer først en række generelle krav til definitioner og anbefaler på den baggrund konkrete justeringer i forhold til den hidtidige danske praksis på området. Arbejdspapiret er blevet til i...

  19. Stabilitet af tynde skalkonstruktioner

    DEFF Research Database (Denmark)

    Laustsen, Bjarke

    for skalkonstruktioner, hvor der kun kræves kendskab til tensornotation og elementær variationsregning. I første del af afhandlingen er den nye teori benyttet til udvikling af en stabilitetsteori. Den ligevægtstilstand hvis stabilitet ønskes undersøgt kan ofte bestemmes vha. en lineær teori. Her er...... forskydningskræfternes bidrag til deformationer negligeret, og ligevægt er opskrevet i den udeformerede tilstand. Den klassiske stabilitetsteori fremkommer vha. den nye teori ved at opstille ligevægtsligninger for nærliggende ligevægtstilstande. Disse fremkommer ved variation af stedvektoren. Teorien kræver, som alle...... størrelser, som er styrende. Desuden udvikles en tilnærmet ikke-lineære teori, hvor det bliver vist, hvordan der kan tages hensyn til imperfektioner. I anden del af afhandlingen benyttes den udledte teori på en aksialt belastet cirkulær cylinderskal. De numeriske beregninger udføres vha. differensmetoden...

  20. Benchmark af erhvervsuddannelserne

    DEFF Research Database (Denmark)

    Bogetoft, Peter; Wittrup, Jesper

    I dette arbejdspapir diskuterer vi, hvorledes de danske erhvervsskoler kan benchmarkes, og vi præsenterer resultaterne af en række beregningsmodeller. Det er begrebsmæssigt kompliceret at benchmarke erhvervsskolerne. Skolerne udbyder en lang række forskellige uddannelser. Det gør det vanskeligt...

  1. Benchmarking af kommunernes sagsbehandling

    DEFF Research Database (Denmark)

    Amilon, Anna

    Fra 2007 skal Ankestyrelsen gennemføre benchmarking af kommuernes sagsbehandlingskvalitet. Formålet med benchmarkingen er at udvikle praksisundersøgelsernes design med henblik på en bedre opfølgning og at forbedre kommunernes sagsbehandling. Dette arbejdspapir diskuterer metoder for benchmarking...

  2. Opfindelsen af naturen

    DEFF Research Database (Denmark)

    Thomsen, Torsten Bøgh

    2016-01-01

    Kritisk omtale og kontekstualisering af Andrea Wulfs, "The Invention of Nature: Alexander von Humboldt’s New World". Der er en direkte forbindelse imellem oplysningstidens naturforståelse og den nuværende klimakrisevirkelighed. Alexander von Humboldt opfandt ikke bare naturen, han påpegede også det...

  3. Comparative distribution of binding of the muscarinic receptor ligands pirenzepine, AF-DX 384, (R,R)-I-QNB and (R,S)-I-QNB to human brain.

    Science.gov (United States)

    Piggott, Margaret; Owens, Jonathan; O'Brien, John; Paling, Sean; Wyper, David; Fenwick, John; Johnson, Mary; Perry, Robert; Perry, Elaine

    2002-09-01

    Quinuclidinyl benzilate (QNB) and its derivatives are being developed to investigate muscarinic receptor changes in vivo in Alzheimer's disease and dementia with Lewy bodies. This is the first study of [125I]-(R,R)-I-QNB and [125I]-(R,S)-I-QNB binding in vitro in human brain. We have compared the in vitro binding of the muscarinic ligands [3H]pirenzepine and [3H]AF-DX 384, which have selectivity for the M1 and M2/M4 receptor subtypes, respectively, to the binding of [125I]-(R,R)-I-QNB and [125I]-(R,S)-I-QNB. This will provide a guide to the interpretation of in vivo SPET images generated with [123I]-(R,R)-I-QNB and [123I]-(R,S)-I-QNB. Binding was investigated in striatum, globus pallidus, thalamus and cerebellum, and cingulate, insula, temporal and occipital cortical areas, which show different proportions of muscarinic receptor subtypes, in post-mortem brain from normal individuals. M1 receptors are of high density in cortex and striatum and are relatively low in the thalamus and cerebellum, while M4 receptors are mainly expressed in the striatum, and M2 receptors are most evident in the cerebellum and thalamus. [125I]-(R,R)-I-QNB and [125I]-(R,S)-I-QNB density distribution patterns were consistent with binding to both M1 and M4 receptors, with [125I]-(R,R)-I-QNB additionally binding to a non-cholinergic site not displaceable by atropine. This distribution can be exploited by in vivo imaging, developing ligands for both SPET and PET, to reveal muscarinic receptor changes in Alzheimer's disease and dementia with Lewy bodies during the disease process and following cholinergic therapy.

  4. Nibe Bredning, klapning af oprensningsmaterialer og vurdering af sedimentspredning

    DEFF Research Database (Denmark)

    Larsen, Torben; Bentzen, Thomas Ruby

    Formålet med undersøgelsen er at beregne spredningen af klapmateriale oprenset fra sejlrenden til Nibe samt Draget. Klappladsen for begge lokaliteter er umiddelbart nordvest for Klitgaard Hage. Beregningen er foretaget vha. af numerisk modellering af strømforhold samt sedimenttransport. Til formå...

  5. Forekomst af resistente bakterier og forbrug af antibiotika til hunde

    DEFF Research Database (Denmark)

    Pedersen, Karl; Pedersen, Kristina; Jensen, Helene

    2007-01-01

    Forekomsten af antibiotikaresistens i forskellige patogene bakterier fra hunde blev bestemt og resultaterne sammenholdt med forbrug af antibiotika til hunde i Danmark. I undersøgelsen indgik isolater af Staphylococcus intermedius (n=201), Streptococcus canis (n=37), Pseudomonas aeruginosa (n=39),...

  6. General strategy for development of fuel cell technology in Denmark; Overordnet strategi for udvikling af braendselscelleteknologi i Danmark

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    This strategy memo on fuel cell technology R and D is prepared by the Danish Energy Authority, Elkraft System and Eltra. The objective of the strategy process is to ensure that publicly funded research is efficient and coordinated with commercial development efforts, and, furthermore, in line with societal objectives for energy related R and D. The first strategy phase focused on establishing an updated overview of Danish R and D regarding fuel cells and evaluation of the perspectives for future use. In the second phase the strategy for fuel cell technology presented in this memo was prepared. The strategy is general and includes two tracks, 'Solid Oxide Fuel Cells' (SOFC) and 'Proton Exchange Membrane Fuel Cells' (PEMFC). A general strategy for publicly funded fuel cell projects should contribute to establish overview and targeting throughout the programme in order to ensure the best possible coordination and efficient use of R and D investments. (BA)

  7. The targeted inhibitory effects of human amniotic fluid stem cells carrying CXCR4 promoter and DAL-1 on non-small cell lung carcinoma growth.

    Science.gov (United States)

    Li, L; Li, S; Cai, T; Wang, H; Xie, X; Liu, Z; Zhang, Y

    2016-02-01

    The differentially expressed in adenocarcinoma of the lung-1 (DAL-1) protein has been demonstrated to be suppressive to various types of tumors including lung cancer. This study aimed to determine the targeted effects of human amniotic fluid stem cells (hAFS cells) carrying CXCR4 promoter driven conditionally replicable adenovirus vector overexpressing DAL-1 (Ad-CXCR4-DAL-1) on non-small cell lung carcinoma (NSCLC) growth. The apoptotic effects of virus vectors were assessed using flow cytometry, and the cytotoxicity analyzed by CCK-8 assay. In vivo imaging system was used to determine the homing capability of hAFS cells. A549 cell xenograft mouse model was created to assess the in vivo effect of DAL-1 overexpression on NSCLC growth. We found that infection of Ad-CXCR4-DAL-1 increased the apoptosis of A549 NSCLC cells but not 16HBE normal human bronchial epithelial cells. Ad-CXCR4-DAL-1 administered via intratumoral injection led to significant reduced growth and greater necrosis of A549 xenograft tumors comparing to null vector treated animals. When infused via tail vein, hAFS cells carrying Ad-CXCR4-DAL-1 homed to lung cancer xenografts, caused virus replication and DAL-1 overexpression, and led to significant lower growth and greater necrosis of A549 cell xenografts comparing to non-treatment control. In conclusion, hAFS cells are capable of carrying Ad-CXCR4-DAL-1 vectors, specifically targeting to lung cancer, and causing oncolytic effects when administered in vivo.

  8. Poser af papir og plast

    DEFF Research Database (Denmark)

    Thygesen, Jette

    2007-01-01

    Artiklen behandler de problemstillinger der er i forbindelse med fortolkning af det afgiftspligtige vareområde. I henhold til lov om afgift af visse emballager, poser, engangsservice, og pvc-folier, svares der afgift af poser, såfremt de frembyder bestemte karakteristika, herunder særligt om de er...

  9. Monitering af korrosion i fjernvarmesystemer

    DEFF Research Database (Denmark)

    Hilbert, Lisbeth Rischel; Nielsen, Lars Vendelbo; Thorarinsdottir, R.I.

    2004-01-01

    Kontrol af korrosion i fjernvarmesystemer foregår primært ved kontrol af vandkvaliteten. Generel korrosion af kulstofstål undgås således normalt ved at holde iltkoncentration og ledningsevne lav og pH høj. Lokaliseret korrosion forekommer dog i systemerne og disse korrosionsangreb detekteres i fo...

  10. Udlejers godkendelse af ny erhvervslejer

    DEFF Research Database (Denmark)

    Munk-Hansen, Carsten

    2009-01-01

    I artiklen er taget udgangspunkt i den »klassiske« afståelsessituation, hvor en lejer vil afhænde den i de lejede lokaler drevne virksomhed, og lejeren har afståelsesret til en af udlejer godkendt lejer, uden at kravene til den ny lejer er specificeret. På baggrund af Vestre Landsrets dom af 9/2 ...

  11. Dannelsen af den ansvarlige elev

    DEFF Research Database (Denmark)

    Larsen, Jesper

    2010-01-01

    Denne artikel handler om den del af lærergerningen, der har at gøre med udarbejdelse af elevplaner. Der tages udgangspunkt i en foucaultsk forståelse, hvor beskrivelsen af den enkelte elev udtrykker et særligt normativt ideal om, hvilke former for elevhed der er de ønskværdige i den danske...

  12. Generation of Human Induced Pluripotent Stem Cells from Extraembryonic Tissues of Fetuses Affected by Monogenic Diseases.

    Science.gov (United States)

    Spitalieri, Paola; Talarico, Rosa V; Botta, Annalisa; Murdocca, Michela; D'Apice, Maria Rosaria; Orlandi, Augusto; Giardina, Emiliano; Santoro, Massimo; Brancati, Francesco; Novelli, Giuseppe; Sangiuolo, Federica

    2015-08-01

    The generation of human induced pluripotent stem cells (hiPSCs) derived from an autologous extraembryonic fetal source is an innovative personalized regenerative technology that can transform own-self cells into embryonic stem-like ones. These cells are regarded as a promising candidate for cell-based therapy, as well as an ideal target for disease modeling and drug discovery. Thus, hiPSCs enable researchers to undertake studies for treating diseases or for future applications of in utero therapy. We used a polycistronic lentiviral vector (hSTEMCCA-loxP) encoding OCT4, SOX2, KLF4, and cMYC genes and containing loxP sites, excisible by Cre recombinase, to reprogram patient-specific fetal cells derived from prenatal diagnosis for several genetic disorders, such as myotonic dystrophy type 1 (DM1), β-thalassemia (β-Thal), lymphedema-distichiasis syndrome (LDS), spinal muscular atrophy (SMA), cystic fibrosis (CF), as well as from wild-type (WT) fetal cells. Because cell types tested to create hiPSCs influence both the reprogramming process efficiency and the kinetics, we used chorionic villus (CV) and amniotic fluid (AF) cells, demonstrating how they represent an ideal cell resource for a more efficient generation of hiPSCs. The successful reprogramming of both CV and AF cells into hiPSCs was confirmed by specific morphological, molecular, and immunocytochemical markers and also by their teratogenic potential when inoculated in vivo. We further demonstrated the stability of reprogrammed cells over 10 and more passages and their capability to differentiate into the three embryonic germ layers, as well as into neural cells. These data suggest that hiPSCs-CV/AF can be considered a valid cellular model to accomplish pathogenesis studies and therapeutic applications.

  13. Changes with Age and the Effect of Recombinant Human BMP-2 on Proteoglycan and Collagen Gene Expression in Rabbit Anulus Fibrosus Cells

    Institute of Scientific and Technical Information of China (English)

    Qin-Ming FEI; Xiao-Xing JIANG; Tong-Yi CHEN; Jun LI; Hideki MURAKAMI; Kai-Jow TSAI; William C. HUTTON

    2006-01-01

    In order to compare the difference between young and old intervertebral disc cells and their responsiveness to recombinant human bone morphogenetic protein-2 (rhBMP-2), disc cells were isolated from the anulus fibrosus (AF) and transition zones of lumbar discs from eight old and eight young New Zealand white rabbits. Compared with the cells from the young rabbits, cells from old rabbits respond less to rhBMP-2 treatment with respect to sulfated-glycosaminoglycan (sGAG) synthesis and aggrecan gene expression. But in collagen Ⅰ and collagen Ⅱ gene expressions, there are no significant differences between the old and the young. When comparing sGAG content, aggrecan, and collagen Ⅱ gene expression of the old AF cells after rhBMP-2 treatment with that of the young AF cells without rhBMP-2 treatment, the old AF cells with rhBMP-2 treatment have a greater capacity to synthesize sGAG bound in the cells and to release sGAG in the media, as well as to express aggrecan and collagen Ⅱ gene. It can be concluded that old AF cells after rhBMP-2 treatment have a greater capacity to synthesize sGAG and express aggrecan and collagen Ⅱ as compared to young AF cells without rhBMP-2 treatment. Thus rhBMP-2 can reverse the decline in the anabolic capacity of the disc cells with ageing. So it seems that rhBMP-2 has potential for use as an agent to retard a key component of disc degeneration and loss of disc matrix.

  14. UHM–ULM interactions in the RBM39–U2AF65 splicing-factor complex

    Energy Technology Data Exchange (ETDEWEB)

    Stepanyuk, Galina A.; Serrano, Pedro; Peralta, Eigen; Farr, Carol L.; Axelrod, Herbert L.; Geralt, Michael; Das, Debanu; Chiu, Hsiu-Ju; Jaroszewski, Lukasz; Deacon, Ashley M.; Lesley, Scott A.; Elsliger, Marc-André; Godzik, Adam; Wilson, Ian A.; Wüthrich, Kurt; Salomon, Daniel R.; Williamson, James R.

    2016-03-24

    RNA-binding protein 39 (RBM39) is a splicing factor and a transcriptional co-activator of estrogen receptors and Jun/AP-1, and its function has been associated with malignant progression in a number of cancers. The C-terminal RRM domain of RBM39 belongs to the U2AF homology motif family (UHM), which mediate protein–protein interactions through a short tryptophan-containing peptide known as the UHM-ligand motif (ULM). Here, crystal and solution NMR structures of the RBM39-UHM domain, and the crystal structure of its complex with U2AF65-ULM, are reported. The RBM39–U2AF65 interaction was confirmed by co-immunoprecipitation from human cell extracts, by isothermal titration calorimetry and by NMR chemical shift perturbation experiments with the purified proteins. When compared with related complexes, such as U2AF35–U2AF65 and RBM39–SF3b155, the RBM39-UHM–U2AF65-ULM complex reveals both common and discriminating recognition elements in the UHM–ULM binding interface, providing a rationale for the known specificity of UHM–ULM interactions. This study therefore establishes a structural basis for specific UHM–ULM interactions by splicing factors such as U2AF35, U2AF65, RBM39 and SF3b155, and a platform for continued studies of intermolecular interactions governing disease-related alternative splicing in eukaryotic cells.

  15. Anmeldelse af Evolution, Literature and Film

    DEFF Research Database (Denmark)

    Grodal, Torben Kragh

    2011-01-01

    Diskussion af basisproblemer i evolutionær fiktionsteori med udgangspunkt i en anmeldelse af Evolution, Literature and Film......Diskussion af basisproblemer i evolutionær fiktionsteori med udgangspunkt i en anmeldelse af Evolution, Literature and Film...

  16. Hætten af for Robin Hood

    DEFF Research Database (Denmark)

    Oxenbøll, Morten

    2010-01-01

    En historisk gennemgang af Robin Hood myten i anledning af den kommende Hood film med Russell Crowe i hovedrollen.......En historisk gennemgang af Robin Hood myten i anledning af den kommende Hood film med Russell Crowe i hovedrollen....

  17. En genlæsning af John Rawls i lyset af hans diskussion af forholdet til fremtidige generationer

    DEFF Research Database (Denmark)

    Lübcke, Poul

    2009-01-01

    På grundlag af en konsekventialistisk rekonstruktion af Rawls' politiske position gøres der rede for Rawls eksplicitte og implicitte vurdering af vores ansvar over for fremtidige generationer......På grundlag af en konsekventialistisk rekonstruktion af Rawls' politiske position gøres der rede for Rawls eksplicitte og implicitte vurdering af vores ansvar over for fremtidige generationer...

  18. Opbygning af alliance

    DEFF Research Database (Denmark)

    Pedersen, Inge Nygaard

    2002-01-01

    Denne artikel er et forsøg på at bringe læseren med ind i atmosfæren, i oplevelsen og overvejelserne i forbindelse med opstart på et musikterapiforløb med en patient, som er meget svær at få kontakt med. Den er skrevet i et detaljerigt praksisnært sprog og har på én gang til for mål at starte på...... dokumentation af musikterapeutisk arbejde i børnepsykiatrien på musikterapiklinikken, APS og at formidle de mange facetter i et allianceopbyggende arbejde med en meget kontaktsvag patient. Artiklen beskriver prøveforløb og første del af et behandlingsforløb og er bygget op, således at praksisbeskrivelser fra...

  19. Coaching af sygedagpengemodtagere

    DEFF Research Database (Denmark)

    Coop Henriksen, Annemette

    SFI gennemførte i foråret 2008 til foråret 2009 en pilotundersøgelse om coaching. Undersøgelsen var designet som et lodtrækningsforsøg og omfattede 42 kvindelige sygedagpengemodtagere fra Rødovre Jobcenter, der var sygemeldt med psykiske lidelser i form af stress, depression eller udbrændthed eller...... med lidelser i bevægeapparatet. Undersøgelsen er bestilt og finansieret af Rødovre Jobcenter. I rapporten undersøges, om coaching kan bidrage til at bringe sygedagpengemodtagere i arbejde eller tættere på arbejdsmarkedet målt ved, om deltagerne får fx øget motivation, mere selvtillid, øget afklaring...

  20. Nedtrykt af negative nyheder

    DEFF Research Database (Denmark)

    Skovsgaard, Morten; Søberg, Pernille Frantz

    2016-01-01

    I adskillige år er det blevet debatteret, hvorvidt nyhedernes negative fokus har konsekvenser for borgerne, og om det i sid-ste ende får flere til at vende ryggen til nyhederne. Vores viden om effekterne af positive og negative nyheder er dog begrænset, og derfor undersøges det i denne artikel......, hvordan henholdsvis positive og negative tv-nyheder påvirker seernes humør, hukom-melse af information fra indslaget og lyst til at se yderligere tv-nyheder. Det gør vi i et survey-eksperiment (N=204), hvor tre grupper så enten et originalt indslag eller det samme indslag klippet med henholdsvis et...

  1. Aflivning af heste

    DEFF Research Database (Denmark)

    Karlshøj, Merete; Fredsbo, Louise; Buhl, Rikke

    2007-01-01

    Det er efterhånden sjældent at heste dør af alderdøm. Ofte må ejer træffe den vanskelige beslutning om at lade hesten aflive. Da der tit er tale om en kær ven, er hesteejeren følelsesmæssigt påvirket. Det er derfor afgørende, at aflivningen foretages fagligt korrekt og professionelt.......Det er efterhånden sjældent at heste dør af alderdøm. Ofte må ejer træffe den vanskelige beslutning om at lade hesten aflive. Da der tit er tale om en kær ven, er hesteejeren følelsesmæssigt påvirket. Det er derfor afgørende, at aflivningen foretages fagligt korrekt og professionelt....

  2. Mesenkymale stamceller. Potentielle anvendelser i celle- og genterapi ved knogletab forårsaget af aldring og osteoporose

    DEFF Research Database (Denmark)

    Justesen, J; Dokkedahl, Karin Stenderup; Kassem, M S

    2001-01-01

    Mesenchymal stem cells (MSC) originate from bone marrow and give rise to various cell types, including osteoblasts, chondrocytes, adipocytes, and myocytes. Lineage-specific differentiation is dependent on activation of specific transcription factors. MSCs play an important role in bone modelling...... to augment healing of bone fractures. Some animal studies have shown that MSCs infused intravenously target bone and possibly participate in bone formation. These studies represent the beginning of a new era, where transplantation with autologous MSCs or genetically-modified MSCs expanded in vitro...

  3. Optisk kvantificering af gopler

    DEFF Research Database (Denmark)

    Christensen, Jens Tang

    efterfølgende med specielt udviklet software, med henblik på arts-, størrelses- og antalsfordeling. Indledende tests har vist, at mens Aurelia og Cyanea nemt registreres i lysgardinet, idet de lyser kraftigt op, er brydningsindekset for små individer af Mnemiopsis så tæt på vand, at der kræves stor kontrast på...

  4. Hvordan integration af it?

    DEFF Research Database (Denmark)

    Toft, Herdis

    2008-01-01

    Fokus i artiklen er den måde, hvorpå børn positionerer sig på i skolerummet, og på, hvordan børn gennem brug af it opbygger og integrerer digital kompetence forstået som færdigheder, kundskaber og holdninger, der skal sætte dem i stand til at agere i det lærende samfund. Med udgangspunkt i et...

  5. Relationel skabelse af ledelsesidentitet

    DEFF Research Database (Denmark)

    Hersted, Lone; Madsen, Charlotte Øland; Michelsen, Randi Riis

    2014-01-01

    forestillinger om, hvad det vil sige at være leder. Disse forestillinger er alle relationelt og kulturelt skabte og præger ofte ledere i deres daglige beslutningstagen og disponering i en sådan grad, at det får betydelige følgevirkninger for resten af organisationen og dens brugere/kunder og øvrige interessenter....

  6. Revisiting the biology of infant t(4;11)/MLL-AF4+ B-cell acute lymphoblastic leukemia

    NARCIS (Netherlands)

    A. Sanjuan-Pla (Alejandra); C. Bueno (C.); C. Prieto (Cristina); P. Acha (Pamela); R.W. Stam (Ronald); R. Marschalek (Rolf); P. Menéndez (Pablo)

    2015-01-01

    textabstractInfant B-cell acute lymphoblastic leukemia (B-ALL) accounts for 10% of childhood ALL. The genetic hallmark of most infant B-ALL is chromosomal rearrangements of the mixed-lineage leukemia (MLL) gene. Despite improvement in the clinicalmanagement and survival (∼85-90%) of childhood B-ALL,

  7. Innovation af innovation

    DEFF Research Database (Denmark)

    Harste, Gorm

    2009-01-01

    , at innovation af innovationen forsøges gennemført på en måde, hvor tiden kræves at forholde sig til sin egen tidslighed i form af fremtid, nutid, fortid og ikke mindst i form af samtidighed. I tiden skal vi iagttage, hvordan vi iagttager tiden. Vi dobbelt-koder tiden på samme måde, som forskning forsker i...... organisationssystemerne. De to typer systemer kan noget helt bestemt med fænomenet tid. De kan synkronisere. Analyseres organisationssystemer ser vi, imidlertid at innovation kræver ro. Stærkt innovative systemer er militærsystemet og kunstsystemet, der også inddrages, og hvor vi ser paradokset mellem innovation og...... involution. Tid er med et medium og ikke et lufttomt rum. Tid er end ikke en gasart, men udgør et solidt fluidum, som samfundet bader i og flyder i, konstant i bevægelse. Reformer forudsætter former, og innovation forudsætter involution. Kun sådan muliggøres evolution....

  8. Anvendelser af Pervasive Communication

    DEFF Research Database (Denmark)

    Brønsted, Jeppe

    I øjeblikket er den måde, vi kommunikerer på, ved at ændre sig drastisk. Fra at vi for få år siden var nødt til at nøjes med Internetkommunikation mellem stationære computere, forbindes en masse nye typer af enheder i dag på nye måder. Da vi nu ikke længere er bundet til en computer, der ikke kan...... flytte sig, bliver en masse nye ting mulige, fordi vi er forbundet med omverdenen i enhver kontekst. Da vi traditionelt ikke tænker i at være forbundet altid overalt er det ikke let at se, hvordan dette nye paradigme påvirker os i vores hverdag og hvordan vi kan udnytte det til nye forretnings- og...... forskningsmuligheder. For at gøre det hele lidt mere konkret, præsenteres i dette dokument en række eksempler på brug af Pervasive Communication. Udvalget af eksempler skal ikke ses som værende udtømmende, men snarere som håndplukkede eksempler der hver repræsenterer en genre. I dokumentet er både valgt...

  9. Innovation af innovation

    DEFF Research Database (Denmark)

    Harste, Gorm

    2009-01-01

    , at innovation af innovationen forsøges gennemført på en måde, hvor tiden kræves at forholde sig til sin egen tidslighed i form af fremtid, nutid, fortid og ikke mindst i form af samtidighed. I tiden skal vi iagttage, hvordan vi iagttager tiden. Vi dobbelt-koder tiden på samme måde, som forskning forsker i...... organisationssystemerne. De to typer systemer kan noget helt bestemt med fænomenet tid. De kan synkronisere. Analyseres organisationssystemer ser vi, imidlertid at innovation kræver ro. Stærkt innovative systemer er militærsystemet og kunstsystemet, der også inddrages, og hvor vi ser paradokset mellem innovation og...... involution. Tid er med et medium og ikke et lufttomt rum. Tid er end ikke en gasart, men udgør et solidt fluidum, som samfundet bader i og flyder i, konstant i bevægelse. Reformer forudsætter former, og innovation forudsætter involution. Kun sådan muliggøres evolution....

  10. Development of integrated DMFC and PEM fuel cell units. Final report; Udvikling af integrerede DMFC og PEM braendselscelle enheder. Slutrapport

    Energy Technology Data Exchange (ETDEWEB)

    Odgaard, M. (IRD Fuel Cell Technology, Svendborg (DK))

    2007-06-15

    The 36-month long project 'Development of integrated DMFC and PEM fuel cell units' has been completed. The project goal was to develop a completely new MEA concept for integrated PEM and DMFC unit cells with enhanced power density and in this way obtain a price reduction. The integrated unit cell consists of a MEA, a gas diffusion layer with flow fields completed with bipolar plates and seals. The main focus of the present project was to: 1) Develop new catalyst materials fabricated by the use of FSD (flame spray deposition method). 2) Optimisation of the state-of-the-art MEA materials and electrode structure. 3) Implementation of a model to account for the CO poisoning of PEM fuel cells. Results and progress obtained in the project established that the individual unit cell components were able to meet and follow the road map of LT-PEM FC regarding electrode catalyst loading and fulfilled the targets for Year 2006. The project has resulted in some important successes. The highlights are as follows: The project has resulted in some important successes. The highlights are as follows: 1) MEA structure knowledge acquired in the project provide a sound basis for further progress. 2) A novel method for the synthesis of electrode by using flame spray synthesis was explored. 3) Electrochemical and catalytic behaviours of catalysts activity for CH{sub 3}OH explored. 4) Implementation of a sub model to account for the CO poisoning of PEM FC has been developed. 5) Numerical study of the flow distribution in FC manifolds was developed and completed with experimental data. 6) The electrode catalyst loading targets for year 2006 achieved. 7) The DMFC MEA performance has been improved by 35%. 8) Optimisation of the MEAs fabrication process has been successfully developed. 9) A new simple flow field design has been designed. 10) A procedure for integrated seals has been developed (au)

  11. Human fetal mesenchymal stem cells.

    Science.gov (United States)

    O'Donoghue, Keelin; Chan, Jerry

    2006-09-01

    Stem cells have been isolated at all stages of development from the early developing embryo to the post-reproductive adult organism. However, the fetal environment is unique as it is the only time in ontogeny that there is migration of stem cells in large numbers into different organ compartments. While fetal neural and haemopoietic stem cells (HSC) have been well characterised, only recently have mesenchymal stem cells from the human fetus been isolated and evaluated. Our group have characterised in human fetal blood, liver and bone marrow a population of non-haemopoietic, non-endothelial cells with an immunophenotype similar to adult bone marrow-derived mesenchymal stem cells (MSC). These cells, human fetal mesenchymal stem cells (hfMSC), are true multipotent stem cells with greater self-renewal and differentiation capacity than their adult counterparts. They circulate in first trimester fetal blood and have been found to traffic into the maternal circulation, engrafting in bone marrow, where they remain microchimeric for decades after pregnancy. Though fetal microchimerism has been implicated in the pathogenesis of autoimmune disease, the biological role of hfMSC microchimerism is unknown. Potential downstream applications of hfMSC include their use as a target cell for non-invasive pre-natal diagnosis from maternal blood, and for fetal cellular and gene therapy. Using hfMSC in fetal therapy offers the theoretical advantages of avoidance of immune rejection, increased engraftment, and treatment before disease pathology sets in. Aside from allogeneic hfMSC in utero transplantation, the use of autologous hfMSC has been brought a step forward with the development of early blood sampling techniques, efficient viral transduction and clonal expansion. Work is ongoing to determine hfMSC fate post-transplantation in murine models of genetic disease. In this review we will examine what is known about hfMSC biology, as well as discussing areas for future research. The

  12. R and D of 2, generation PEC solar cells; F/U af 2. generations PEC solceller

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    The report summarizes results from the project 'R and D of 2nd generation PEC solar cells'. The project has focused strongly on development and implementation of improved counter- and photo-electrodes for PEC solar cells. This has resulted in an alternative counter-electrode which is stable in production and potentially cheaper to produce than the platinum counter-electrode which currently is the most popular. Furthermore, the project has focused on developing a rational method to produce a photo-electrode from commercially available raw materials. The idea is to reduce the number of process steps as compared to processes described in literature. The newly developed electrodes have been designed with a view to enter into a coupled system in which they are built together in a monolithic structure. For this purpose a coupled electrode system has been designed and tested. The electrodes have also been designed to subsequent micro structuring, which contributes to an increase in the solar cells yield and durability. Finally, a flexible production platform has been developed on which the number of processes have been significantly reduced. (BA)

  13. Differentiated human stem cells resemble fetal, not adult, β cells.

    Science.gov (United States)

    Hrvatin, Sinisa; O'Donnell, Charles W; Deng, Francis; Millman, Jeffrey R; Pagliuca, Felicia Walton; DiIorio, Philip; Rezania, Alireza; Gifford, David K; Melton, Douglas A

    2014-02-25

    Human pluripotent stem cells (hPSCs) have the potential to generate any human cell type, and one widely recognized goal is to make pancreatic β cells. To this end, comparisons between differentiated cell types produced in vitro and their in vivo counterparts are essential to validate hPSC-derived cells. Genome-wide transcriptional analysis of sorted insulin-expressing (INS(+)) cells derived from three independent hPSC lines, human fetal pancreata, and adult human islets points to two major conclusions: (i) Different hPSC lines produce highly similar INS(+) cells and (ii) hPSC-derived INS(+) (hPSC-INS(+)) cells more closely resemble human fetal β cells than adult β cells. This study provides a direct comparison of transcriptional programs between pure hPSC-INS(+) cells and true β cells and provides a catalog of genes whose manipulation may convert hPSC-INS(+) cells into functional β cells.

  14. Comparison of human amniotic fluid-derived and umbilical cord Wharton's Jelly-derived mesenchymal stromal cells: Characterization and myocardial differentiation capacity

    Institute of Scientific and Technical Information of China (English)

    Jing Bai; Yuan Hu; Yi-Ru Wang; Li-Feng Liu; Jie Chen; Shao-Ping Su; Yu Wang

    2012-01-01

    Objective To compare the characterization and myocardial differentiation capacity of amniotic fluid-derived mesenchymal stromal cells (AF MSCs) and umbilical cord Wharton's Jelly-derived mesenchymal stromal cells (WJ MSCs). Methods The human AF MSCs were cultured from amniotic fluid samples obtained by amniocentesis. The umbilical cord WJ MSCs were obtained from Wharton's Jelly of umbilical cords of infants delivered full-term by normal labor. The morphology, growth curves, and analyses by flow cytometry of cell surface markers were compared between the two types of cells. Myocardial genes (GATA-4, c-TnT, α-actin, and Cx43) were detected by real-time PCR and the corresponding protein expressions were detected by Western blot analysis after myocardial induced in AF MSCs and WJ MSCs. Results Our findings revealed AF MSCs and WJ MSCs shared similar morphological characteristics of the fibroblastoid shape. The AF MSCs were easily obtained than the WJ MSCs and had a shorter time to reach adherence of 2.7 ± 1.6 days to WJ MSCs of 6.5 ± 1.8 days. The growth curves by MTT cytotoxic assay showed the AF MSCs had a similar proliferative capacity at passage 5 and passage 10. However, the proliferative capacities of WJ MSCs were decreased at 5 passage relative to 10 passage. Both AF stem cells and WJ stem cells had the characteristics of mesenchymal stromal cells with some characteristics of embryonic stem cells. They express CD29 and CD105, but not CD34. They were positive for Class I major histocompatibility (MHC I) antigens (HLA-ABC), and were negative, or mildly positive, for MHC Class II (HLA-DR) antigen. Oct-4 was positive in all the two cells types. Both AF MSCs and WJ MSCs could differentiate along myocardium. The differentiation capacities were detected by the expression of GATA-4, c-TnT, α-actin, Cx43 after myocardial induction. Conclusions Both AF MSCs and WJ MSCs have the potential clinical application for myogenesis in cardiac regenerative therapy.

  15. Reduktion af metanemissionen fra Klintholm losseplads ved etablering af biocover

    DEFF Research Database (Denmark)

    Pedersen, Rasmus Broen; Scheutz, Charlotte; Kjeldsen, Peter

    Rapporten omhandler etableringen og funktionen af et biocover på deponietape 0 på lossepladsen Klintholm I/S. Biocoveret blev etableret som en del af et samlet projekt for slutafdækning og gashåndtering. Projektets hovedformål var at nedsætte deponietapens metanemission med en faktor 10. Virkning....... Virkningsgraden blev dokumenteret vha. sporstofmålinger af deponiets samlede metanemission før, under og efter etableringen af biocoveret. Emissionen faldt fra 8,3 kg/t til 1,2 kg/t svarende til en reduktion på ca. 86 %....

  16. Inhibition of glycogen synthase kinase-3 (GSK3) promotes the neural differentiation of full-term amniotic fluid-derived stem cells towards neural progenitor cells.

    Science.gov (United States)

    Gao, Liyang; Zhao, Mingyan; Ye, Wei; Huang, Jinzhi; Chu, Jiaqi; Yan, Shouquan; Wang, Chaojun; Zeng, Rong

    2016-08-01

    The amniotic fluid has a heterogeneous population of cells. Some human amniotic fluid-derived stem (hAFS) cells have been shown to harbor the potential to differentiate into neural cells. However, the neural differentiation efficiency of hAFS cells remains low. In this study, we isolated CD117-positive hAFS cells from amniotic fluid and then examined the pluripotency of these cells through the formation of embryoid bodies (EBs). Additionally, we induced the neural differentiation of these cells using neuroectodermal medium. This study revealed that the GSK3-beta inhibitor SB216763 was able to stimulate the proliferation of CD117-positive hAFS cells without influencing their undifferentiated state. Moreover, SB216763 can efficiently promote the neural differentiation of CD117-positive hAFS cells towards neural progenitor cells in the presence of DMEM/F12 and N2 supplement. These findings provide an easy and low-cost method to maintain the proliferation of hAFS cells, as well as induce an efficacious generation of neural progenitor cells from hAFS cells. Such induction of the neural commitment of hAFS cells may provide an option for the treatment of neurodegenerative diseases by hAFS cells-based therapies.

  17. Korrosionshastigheder af rene zinkcoatings

    DEFF Research Database (Denmark)

    Bech-Nielsen, G.; Tang, Peter Torben; Ulrich, Dan

    1991-01-01

    Det har længe været den fremherskende opfattelse i galvanobranchen, at et zinkbad var et zinkbad og at der, tiltrods for de forskellige leverandørers gyldne løfter, ikke var den store forskel på de bade der kan købes på markedet idag. Det har ydermere været den almindelige opfattelse, at zinkbade......-bestandighed og udseende. Badenes alder har også vist sig at have endog meget stor betydning for kvaliteten af belægningerne....

  18. Kulturelle spejlinger af Profeten Muhammed

    DEFF Research Database (Denmark)

    Høgsbro Lading, Åse

    2008-01-01

    Artiklen er en analyse af to forløb i den såkaldte Muhammedkrise, der udspringer af Jyllandspostens offentliggørelse af karrikaturer af profeten Muhammed. Den første i 2006 ses som et  udspil, der først og frememst er styret  af markedsmæssige interesser, og hvor de globale reaktioner kom bag på ...... forsvar opleves  af avisen i splitting-kategorier: enten kan man sige alt, eller også har man ikke ytringsfrihed. Artiklen slutter med et ønske om, at mere komplekse syn på de globale relationer kan etableres...

  19. The potency of human testicular stem cells

    NARCIS (Netherlands)

    Chikhovskaya, J.V.

    2013-01-01

    In this thesis, we evaluate the stem cell state of cells present in primary human testicular cell cultures as well as their origin and relation to germ or somatic lineages within testicular tissue. We conclude that human testis-derived embryonic stem cell-like (htES-like) colonies arising in primary

  20. Anvendelse af MIP ved kildekarakterisering og vurdering af nedbrydning af chlorerede opløsningsmidler

    DEFF Research Database (Denmark)

    Broholm, Mette Martina; Janniche, Gry Sander; Damgaard, Ida;

    2014-01-01

    Membrane Interface Probing (MIP) er et 'direct push' screeningsværktøj, som kan anvendes ved karakterisering af forurenede grunde. Karakterisering af kildeområder med chlorerede opløsningsmidler (som DNAPL) udgør et vigtigt led i udviklingen af den konceptuelle forståelse, som er essentiel...... for risikovurdering og valg af afværgestrategi. Integreret karakterisering med en række metoder for direkte såvel som indirekte dokumentation af DNAPL i moræneler, herunder MIP, er beskrevet i Kerrn-Jespersen et al. (2013). Det samlede DNAPL-karakteriseringsprojekt er beskrevet i detaljer i Janniche et al. (2013). En...... vurdering af anvendte detektorer for MIP er foretaget af Olsson (2013). Ved karakterisering af forurening med bl.a. chlorerede opløsningsmidler er der behov for en vurdering af forureningssammensætning og nedbrydning. Tilsvarende vurdering er nødvendig, når udviklingen af stimuleret reduktiv dechlorering...

  1. En verden drevet af vindkraft

    DEFF Research Database (Denmark)

    Aukdal, Kenneth Martin

    2000-01-01

    For over 100 år siden opstillede Poul la Cour og et hold videnskabsmænd den første testvindmølle i Danmark. Det var den spæde start til en udvikling, der har gjort Danmark til verdens førende eksportør af vindkraftteknologi. Over 60% af den elektricitet,der produceres af vindmøller i verden i dag...

  2. Sammenligning af energiforbrug i supermarkeder

    DEFF Research Database (Denmark)

    Knudsen, Hans Jørgen Høgaard

    2005-01-01

    Formålet med det her omtalte projekt har været at sammenligne energiforbruget til køling i fire supermarkeder af sammenlignelig størrelse men med forskellig opbygning af køleanlægget. To af supermarkederne har nyudviklede kaskadeanlæg med CO2 i lavtemperaturkredsen og R404A i højtemperaturkredsen...

  3. Slaver af Væksten

    DEFF Research Database (Denmark)

    Nørgaard, Jørgen

    2004-01-01

    Artiklen rejser spørgsmålet om der, foruden den miljømæssige nødvendighed af at bremse væksten i Brutto National Produktet, også er direkte meneskelige fordele ved et sådant skridt. Dette aspekt er illustreret med de stigende negative følger af velstanden i USA og andre overflodslande, i form af ...

  4. Centralisering overfor decentralisering af varelagre

    DEFF Research Database (Denmark)

    Graungaard Pedersen, Søren; Zachariassen, Frederik

    2010-01-01

    store virksomheder. I Danmark går langt størstedelen af virksomheder under betegnelsen små og mellemstore virksomheder (SMV) og for en stor andel af disse, er udfordringen med effektiv styring af varelagre også relevant. Denne artikel beskæftiger sig med udfordringen mellem centralisering overfor...... decentralisering af varelagre i SMV. Det viser sig, at der er betydelige forskelle i, hvilke argumenter der er for og imod, når udfordringen ses fra et SMV-perspektiv....

  5. Coaching af sygedagpengemodtagere

    DEFF Research Database (Denmark)

    Coop Henriksen, Annemette

    SFI gennemførte i foråret 2008 til foråret 2009 en pilotundersøgelse om coaching. Undersøgelsen var designet som et lodtrækningsforsøg og omfattede 42 kvindelige sygedagpengemodtagere fra Rødovre Jobcenter, der var sygemeldt med psykiske lidelser i form af stress, depression eller udbrændthed eller...... med lidelser i bevægeapparatet. Undersøgelsen er bestilt og finansieret af Rødovre Jobcenter. I rapporten undersøges, om coaching kan bidrage til at bringe sygedagpengemodtagere i arbejde eller tættere på arbejdsmarkedet målt ved, om deltagerne får fx øget motivation, mere selvtillid, øget afklaring...... og færre symptomer på sygdom. Undersøgelsen viser, at gruppen, der har modtaget coaching, oplever en positiv udvikling i forhold til stress, depression og udbrændthed. Gruppen, der modtog coaching, har den tydeligste positive udvikling, men begge grupper har oplevet en helbredsmæssig fremgang i...

  6. Stem cell differentiation and human liver disease

    Institute of Scientific and Technical Information of China (English)

    Wen-Li Zhou; Claire N Medine; Liang Zhu; David C Hay

    2012-01-01

    Human stem cells are scalable cell populations capable of cellular differentiation.This makes them a very attractive in vitro cellular resource and in theory provides unlimited amounts of primary cells.Such an approach has the potential to improve our understanding of human biology and treating disease.In the future it may be possible to deploy novel stem cell-based approaches to treat human liver diseases.In recent years,efficient hepatic differentiation from human stem cells has been achieved by several research groups including our own.In this review we provide an overview of the field and discuss the future potential and limitations of stem cell technology.

  7. The human airway epithelial basal cell transcriptome.

    Directory of Open Access Journals (Sweden)

    Neil R Hackett

    Full Text Available BACKGROUND: The human airway epithelium consists of 4 major cell types: ciliated, secretory, columnar and basal cells. During natural turnover and in response to injury, the airway basal cells function as stem/progenitor cells for the other airway cell types. The objective of this study is to better understand human airway epithelial basal cell biology by defining the gene expression signature of this cell population. METHODOLOGY/PRINCIPAL FINDINGS: Bronchial brushing was used to obtain airway epithelium from healthy nonsmokers. Microarrays were used to assess the transcriptome of basal cells purified from the airway epithelium in comparison to the transcriptome of the differentiated airway epithelium. This analysis identified the "human airway basal cell signature" as 1,161 unique genes with >5-fold higher expression level in basal cells compared to differentiated epithelium. The basal cell signature was suppressed when the basal cells differentiated into a ciliated airway epithelium in vitro. The basal cell signature displayed overlap with genes expressed in basal-like cells from other human tissues and with that of murine airway basal cells. Consistent with self-modulation as well as signaling to other airway cell types, the human airway basal cell signature was characterized by genes encoding extracellular matrix components, growth factors and growth factor receptors, including genes related to the EGF and VEGF pathways. Interestingly, while the basal cell signature overlaps that of basal-like cells of other organs, the human airway basal cell signature has features not previously associated with this cell type, including a unique pattern of genes encoding extracellular matrix components, G protein-coupled receptors, neuroactive ligands and receptors, and ion channels. CONCLUSION/SIGNIFICANCE: The human airway epithelial basal cell signature identified in the present study provides novel insights into the molecular phenotype and biology of

  8. Relevansen af Aristoteles' etik for konceptionen af eksistentiel terapi

    DEFF Research Database (Denmark)

    Dræby, Anders

    Siden sin undfangelse i begyndelsen af det 20. århundrede har den eksistentielle terapi repræsenteret et filosofisk funderet alternativ til de former for terapeutisk praksis, der er funderet i den funktionalistiske medicinske metafysik. Anvendelse af elementer fra Aristoteles' etik muliggør en...

  9. En systemteoretisk analyse af underviseres medlemskab af uddannelsessteder

    DEFF Research Database (Denmark)

    Kaspersen, Steffen Warming

    2014-01-01

    Afhandlingen undersøger ved hjælp af Niklas Luhmanns systemteori, hvordan undervisere på to uddannelsessteder der skal fusionere, er med til at bidrage til det nye uddannelsesstedets selvbeskrivelse. Der konkluderes, at undervisernes egen iagttagelse af uddannelsesstedet afgør hvilke faktorer der...

  10. Politisk brug af historien og legitimering af nutiden i Jugoslavien

    DEFF Research Database (Denmark)

    Humlebæk, Carsten

    2014-01-01

    Anmeldelse af: Tea Sindbæk: Usable History? Representations of Yugoslavia’s difϔicult past from 1945 to 2002, Aarhus University Press 2013.......Anmeldelse af: Tea Sindbæk: Usable History? Representations of Yugoslavia’s difϔicult past from 1945 to 2002, Aarhus University Press 2013....

  11. Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system

    Directory of Open Access Journals (Sweden)

    E. Bertin

    2015-11-01

    Full Text Available Induced pluripotent stem (iPS cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Amniotic fluid (AF cells are easy to obtain from routinely scheduled procedures for prenatal diagnosis and iPS cells have been generated from human AF. Here, we generated iPS cells from mouse AF cells, using a non-viral-based approach constituted by the PiggyBac (PB transposon system. All iPS cell lines obtained exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo.

  12. Simulering af dagslys i digitale modeller

    DEFF Research Database (Denmark)

    Villaume, René Domine; Ørstrup, Finn Rude

    2004-01-01

    Projektet undersøger via forskellige simuleringer af dagslys, kvaliteten af visualiseringer af komplekse lysforhold i digitale modeller i forbindelse med formidling af arkitektur via nettet. I en digital 3D model af Utzon Associates Paustians hus, simulers naturligt dagslysindfald med  forskellig...... Renderingsmetoder som: "shaded render" /  ”raytraceing” /  "Final Gather /  ”Global Illumination”...

  13. Endocannabinoids and Human Sperm Cells

    Directory of Open Access Journals (Sweden)

    Giovanna Zolese

    2010-10-01

    Full Text Available N-acylethanolamides (NAEs are naturally occurring signaling lipids consisting of amides and esters of long-chain polyunsaturated fatty acids. Usually they are present in a very small amounts in many mammalian tissues and cells, including human reproductive tracts and fluids. Recently, the presence of N-arachidonoylethanolamide (anandamide, AEA, the most characterised member of endocannabinoids, and its congeners palmitoylethanolamide (PEA and oleylethanolamide (OEA in seminal plasma, oviductal fluid, and follicular fluids was demonstrated. AEA has been shown to bind not only type-1 (CB1 and type-2 (CB2 cannabinoid receptors, but also type-1 vanilloid receptor (TRPV1, while PEA and OEA are inactive with respect to classical cannabinoid CB1 and CB2 but activate TRPV1 or peroxisome proliferator activate receptors (PPARs. This review concerns the most recent experimental data on PEA and OEA, endocannabinoid-like molecules which appear to exert their action exclusively on sperm cells with altered features, such as membrane characteristics and kinematic parameters. Their beneficial effects on these cells could suggest a possible pharmacological use of PEA and OEA on patients affected by some forms of idiopathic infertility.

  14. Reformen af erhvervsuddannelserne

    DEFF Research Database (Denmark)

    Jørgensen, Christian Helms

    2016-01-01

    inklusi-on og fastholdelse, som har været i højsædet siden reformen i 2007. Men samtidig gentager reformen på mange områder tidligere reformer, både med hensyn til hvad den udpeger som problemer, og hvad den peger på som løsninger. Reformens indførelse af et todelt grundforløb ligner til forveksling den...... løsning der indførtes med reformen i 1991. Det er dog ikke ren gentagelse. Ofte veksler de politiske reformer mellem to modsatrettede tiltag, som begge fremføres som løsning på det samme problem, for eksempel frafald. Spørgsmålet, der diskuteres i artiklen er, hvorfor reformerne tilsyneladende gentager...

  15. Domesticeringen af Ford i Danmark

    DEFF Research Database (Denmark)

    Wagner, Michael

    med en markedsandel på mere end 50%. En position som Ford måtte afgive til General Motors i løbet af årtiet. I Danmark var mere end hvert andet indregistreret automobil i 1930 antageligt af mærket Ford, og helt frem til verdenskrigens udbrud var Ford Motor Company et brand, som spillede en meget...

  16. Virkelighedsflugt og masser af sex

    DEFF Research Database (Denmark)

    Lorentzen, Rasmus Fink

    2011-01-01

    I artiklen laves en læsning af ungdomsromanen "6" (2010) af Ronni Andersen. Dernæst udfoldes en række litteraturpædagogiske forslag tilen undervisning i romanen i skolens ældste klasser. Arbejdsforslagene er baseret på en kompetencetilgang til danskfaget og rummer fire værksteder hvor eleverne sk...

  17. Monitering af korrosion i fjernvarmesystemer

    DEFF Research Database (Denmark)

    Thorarinsdottir, R.I.; Nielsen, Kate; Nielsen, Lars Vendelbo

    2001-01-01

    Et tre-årigt nordisk projekt er netop startet. Forskere og repræsentanter fra industrien i Danmark, Finland, Island, Norge og Sverige samarbejder i projektet. Projektet har til formål at forbedre monitering af korrosion i fjernvarmesystemer bl.a. ved udvikling af en måleunit....

  18. Dimensionering af plansiloer til ensilage

    DEFF Research Database (Denmark)

    Terpager Andersen, K.

    Der er fortsat stor interesse for bygning af plansiloer til ensilage, og SBI's afdeling for landbrugsbygninger er derfor i gang med at udarbejde et sæt byggeblade herom. For at kunne dimensionere sådanne siloer er det nødvendigt at kende størrelsen af de tryk, ensilagen udøver på silosiderne, og...

  19. Evaluering af den fleksible barselsorlov

    DEFF Research Database (Denmark)

    Marianne Olsen, Bente

    forældreorloven. Undersøgelsen viser også, at der er en række områder, hvor kommunerne mener, at orlovsreglerne udgør en administrativ byrde. Det bliver ekstra tungt, fordi det IT-system, der understøtter administrationen af barselsreglerne, ikke fungerer optimalt. Omring to tredjedel af de adspurgte...

  20. Evalueringen af den fleksible barselorlov

    DEFF Research Database (Denmark)

    Marianne Olsen, Bente

    forældreorloven. Undersøgelsen viser også, at der er en række områder, hvor kommunerne mener, at orlovsreglerne udgør en administrativ byrde. Det bliver ekstra tungt, fordi det IT-system, der understøtter administrationen af barselsreglerne, ikke fungerer optimalt. Omring to tredjedel af de adspurgte...

  1. Anbefalinger til organisering af uddannelserne

    DEFF Research Database (Denmark)

    Andersen, Lars; Andersen, Lars Døvling; Hornemann, Birte C.

    2009-01-01

    Notatet sammenfatter de diskussioner, som det af fakulteterne nedsatte udvalg vedrørende nye undervisnings- og eksamensformer har haft, og de anbefalinger til en omlægning af vores uddannelsessystem, som udvalget har valgt at give. Notatet er samtidig en begyndelse på en erfaringsudveksling om ny...

  2. Rekonstruktion af en mistet identitet

    DEFF Research Database (Denmark)

    Haarder, Jon Helt

    2010-01-01

    CARL FRODE TILLER: INDKREDSNING (INNCIRKLING) Oversat fra nynorsk af Karsten Sand Iversen 300 sider, 299,95 kr. C & K Forlag Er udkommet Fire stjerner.......CARL FRODE TILLER: INDKREDSNING (INNCIRKLING) Oversat fra nynorsk af Karsten Sand Iversen 300 sider, 299,95 kr. C & K Forlag Er udkommet Fire stjerner....

  3. Udvendig efterisolering af en etageejendom

    DEFF Research Database (Denmark)

    Nørregaard, M.; Blad, H.; Christensen, G.

    Rapporten redegør for et projekt, der har til formål at belyse mulighederne for at varmeisolere eksisterende etageejendomme udvendigt. Hovedindholdet af rapporten er en nøje beskrivelse af den facadeisolering, der er gennemført på to ejendomme fra 1890'erne i København. Desuden redegøres for den ...

  4. Midtvejsevaluering af Pulje til Landsbyfornyelse

    DEFF Research Database (Denmark)

    Jensen, Jesper Ole; Blach, Vigdis E. Magnusdatter

    og bidrage til en helhedsorienteret og fremadrettet indsats i en række kommuner i de danske yderområder. Midtvejsevalueringen belyser effekten af puljen og kommunernes erfaringer med administrationen af puljen. Evalueringen er gennemført som en internet-baseret spørgeskemaundersøgelse (survey) blandt...

  5. Kvalifikation af den optiske aftastning

    DEFF Research Database (Denmark)

    Sobiecki, René

    Hvor koordinatmålemaskinen i årtier har indtaget en nøgleposition i en virksomhed til fremskaffelse af måledata, ser vi nu en udvikling i retning af mere og mere optisk måleudstyr. Profilprojektoren er vendt tilbage i en ny avanceret form. Fordele og ulemper ved mekanisk kontra optisk måling er v...

  6. Human regulatory B cells control the TFH cell response.

    Science.gov (United States)

    Achour, Achouak; Simon, Quentin; Mohr, Audrey; Séité, Jean-François; Youinou, Pierre; Bendaoud, Boutahar; Ghedira, Ibtissem; Pers, Jacques-Olivier; Jamin, Christophe

    2017-07-01

    Follicular helper T (TFH) cells support terminal B-cell differentiation. Human regulatory B (Breg) cells modulate cellular responses, but their control of TFH cell-dependent humoral immune responses is unknown. We sought to assess the role of Breg cells on TFH cell development and function. Human T cells were polyclonally stimulated in the presence of IL-12 and IL-21 to generate TFH cells. They were cocultured with B cells to induce their terminal differentiation. Breg cells were included in these cultures, and their effects were evaluated by using flow cytometry and ELISA. B-cell lymphoma 6, IL-21, inducible costimulator, CXCR5, and programmed cell death protein 1 (PD-1) expressions increased on stimulated human T cells, characterizing TFH cell maturation. In cocultures they differentiated B cells into CD138(+) plasma and IgD(-)CD27(+) memory cells and triggered immunoglobulin secretions. Breg cells obtained by Toll-like receptor 9 and CD40 activation of B cells prevented TFH cell development. Added to TFH cell and B-cell cocultures, they inhibited B-cell differentiation, impeded immunoglobulin secretions, and expanded Foxp3(+)CXCR5(+)PD-1(+) follicular regulatory T cells. Breg cells modulated IL-21 receptor expressions on TFH cells and B cells, and their suppressive activities involved CD40, CD80, CD86, and intercellular adhesion molecule interactions and required production of IL-10 and TGF-β. Human Breg cells control TFH cell maturation, expand follicular regulatory T cells, and inhibit the TFH cell-mediated antibody secretion. These novel observations demonstrate a role for the Breg cell in germinal center reactions and suggest that deficient activities might impair the TFH cell-dependent control of humoral immunity and might lead to the development of aberrant autoimmune responses. Copyright © 2016 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  7. Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

    Directory of Open Access Journals (Sweden)

    Anna Jezierski

    2012-01-01

    Full Text Available The usage of stem cells is a promising strategy for the repair of damaged tissue in the injured brain. Recently, amniotic fluid (AF cells have received a lot of attention as an alternative source of stem cells for cell-based therapies. However, the success of this approach relies significantly on proper interactions between graft and host tissue. In particular, the reestablishment of functional brain networks requires formation of gap junctions, as a key step to provide sufficient intercellular communication. In this study, we show that AF cells express high levels of CX43 (GJA1 and are able to establish functional gap junctions with cortical cultures. Furthermore, we report an induction of Cx43 expression in astrocytes following injury to the mouse motor cortex and demonstrate for the first time CX43 expression at the interface between implanted AF cells and host brain cells. These findings suggest that CX43-mediated intercellular communication between AF cells and cortical astrocytes may contribute to the reconstruction of damaged tissue by mediating modulatory, homeostatic, and protective factors in the injured brain and hence warrants further investigation.

  8. Satellite cells in human skeletal muscle plasticity.

    Science.gov (United States)

    Snijders, Tim; Nederveen, Joshua P; McKay, Bryon R; Joanisse, Sophie; Verdijk, Lex B; van Loon, Luc J C; Parise, Gianni

    2015-01-01

    Skeletal muscle satellite cells are considered to play a crucial role in muscle fiber maintenance, repair and remodeling. Our knowledge of the role of satellite cells in muscle fiber adaptation has traditionally relied on in vitro cell and in vivo animal models. Over the past decade, a genuine effort has been made to translate these results to humans under physiological conditions. Findings from in vivo human studies suggest that satellite cells play a key role in skeletal muscle fiber repair/remodeling in response to exercise. Mounting evidence indicates that aging has a profound impact on the regulation of satellite cells in human skeletal muscle. Yet, the precise role of satellite cells in the development of muscle fiber atrophy with age remains unresolved. This review seeks to integrate recent results from in vivo human studies on satellite cell function in muscle fiber repair/remodeling in the wider context of satellite cell biology whose literature is largely based on animal and cell models.

  9. Health Information in Somali (Af-Soomaali )

    Science.gov (United States)

    ... Emergency Plan - Af-Soomaali (Somali) MP4 Healthy Roads Media Mass Casualty Patient Self-Assessment Form - Af-Soomaali (Somali) Bilingual PDF Health Information ... MP4 Healthy Roads Media Pets and Disaster - English Pets and Disaster - Af- ...

  10. IMMUNORESPONSES OF HUMANIZED SCID MICE TO HUMAN LUNG CANCER CELLS

    Institute of Scientific and Technical Information of China (English)

    陈力真; 王树蕙; 张云; 王世真

    1996-01-01

    HuPBL-SCID mice were used to explore how they would response to human ttmoor cells of 801/MLC.Living 801/MLC cells appeared to be fetal to the the mice due to the production of human TNF. The huP-BL-SCID rniee did not generate any noticeable amotmt of specific human immunoglobttlin either by single immunization with living 801/MLC cells or by repeated immunization with irradiated 801/MLC cells. Our preliminary experiments with huPBL-SCID mice showed that such chimeras would he a very useful models for tumor immunological researches.

  11. Search for naive human pluripotent stem cells

    Institute of Scientific and Technical Information of China (English)

    Simone Aparecida Siqueira Fonseca; Roberta Montero Costas; Lygia Veiga Pereira

    2015-01-01

    Normal mouse pluripotent stem cells were originallyderived from the inner cell mass (ICM) of blastocystsand shown to be the in vitro equivalent of those preimplantationembryonic cells, and thus were calledembryonic stem cells (ESCs). More than a decade later,pluripotent cells were isolated from the ICM of humanblastocysts. Despite being called human ESCs, thesecells differ significantly from mouse ESCs, includingdifferent morphology and mechanisms of control ofpluripotency, suggesting distinct embryonic originsof ESCs from the two species. Subsequently, mousepluripotent stem cells were established from the ICMderivedepiblast of post-implantation embryos. Thesemouse epiblast stem cells (EpiSCs) are morphologicaland epigenetically more similar to human ESCs. Thisraised the question of whether cells from the humanICM are in a more advanced differentiation stage thantheir murine counterpart, or whether the availableculture conditions were not adequate to maintain thosehuman cells in their in vivo state, leading to a transitioninto EpiSC-like cells in vitro . More recently, novel cultureconditions allowed the conversion of human ESCs intomouse ESC-like cells called naive (or ground state)human ESCs, and the derivation of naive human ESCsfrom blastocysts. Here we will review the characteristicsof each type of pluripotent stem cells, how (andwhether) these relate to different stages of embryonicdevelopment, and discuss the potential implications ofnaive human ESCs in research and therapy.

  12. Udvikling af CO2 neutralt byrumsarmatur

    DEFF Research Database (Denmark)

    Poulsen, Peter Behrensdorff; Dam-Hansen, Carsten; Corell, Dennis Dan

    Denne rapport indeholder en beskrivelse af arbejdet udført i og resultaterne af forsknings- og udviklingsprojektet ” Udvikling af CO2 neutralt byrumsarmatur” og udgør slutrapportering for dette projekt.......Denne rapport indeholder en beskrivelse af arbejdet udført i og resultaterne af forsknings- og udviklingsprojektet ” Udvikling af CO2 neutralt byrumsarmatur” og udgør slutrapportering for dette projekt....

  13. Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells

    Institute of Scientific and Technical Information of China (English)

    Cheng-Ye; Che; Jing-Fen; Zhang; Ji-Eun; Lee; Jing; Lin; Li-Ting; Hu; Nan; Jiang; Qian; Wang; Qiang; Xu; Gui-Qiu; Zhao

    2015-01-01

    AIM: To evaluate the early expression of mannose-binding lectin 2(MBL2) in human corneal epithelial cells(HCECs) infected by Aspergillus fumigatus(AF).METHODS: HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 m RNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay(ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24 h.RESULTS: MBL2 m RNA and protein are expressed in normal HCECs. The expression of MBL2 m RNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 m RNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of24 h.· CONCLUSION: MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.

  14. Endothelial cells derived from human embryonic stem cells

    Science.gov (United States)

    Levenberg, Shulamit; Golub, Justin S.; Amit, Michal; Itskovitz-Eldor, Joseph; Langer, Robert

    2002-04-01

    Human embryonic stem cells have the potential to differentiate into various cell types and, thus, may be useful as a source of cells for transplantation or tissue engineering. We describe here the differentiation steps of human embryonic stem cells into endothelial cells forming vascular-like structures. The human embryonic-derived endothelial cells were isolated by using platelet endothelial cell-adhesion molecule-1 (PECAM1) antibodies, their behavior was characterized in vitro and in vivo, and their potential in tissue engineering was examined. We show that the isolated embryonic PECAM1+ cells, grown in culture, display characteristics similar to vessel endothelium. The cells express endothelial cell markers in a pattern similar to human umbilical vein endothelial cells, their junctions are correctly organized, and they have high metabolism of acetylated low-density lipoprotein. In addition, the cells are able to differentiate and form tube-like structures when cultured on matrigel. In vivo, when transplanted into SCID mice, the cells appeared to form microvessels containing mouse blood cells. With further studies, these cells could provide a source of human endothelial cells that could be beneficial for potential applications such as engineering new blood vessels, endothelial cell transplantation into the heart for myocardial regeneration, and induction of angiogenesis for treatment of regional ischemia.

  15. Induced pluripotent stem cell lines derived from human somatic cells.

    Science.gov (United States)

    Yu, Junying; Vodyanik, Maxim A; Smuga-Otto, Kim; Antosiewicz-Bourget, Jessica; Frane, Jennifer L; Tian, Shulan; Nie, Jeff; Jonsdottir, Gudrun A; Ruotti, Victor; Stewart, Ron; Slukvin, Igor I; Thomson, James A

    2007-12-21

    Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.

  16. Modellering af elforbrug til netanalyse

    DEFF Research Database (Denmark)

    Østergaard, Jacob

    I denne rapport undersøges mulighederne for at etablere en model for elforbruget, der kan anvendes ved analyser af det østdanske transmissionsnet. Rapporten henvender sig til ingeniører og beslutningstagere, der ønsker viden om betydningen af en nøjagtig elforbrugsmodel og om mulighederne for i p...... simuleringsresultater med mindre usikkerhed, og der opnås en integration mellem eksisterende systemer, idet data fra en række systemer bl.a. Panda, elforbrugsprognoser, elforbrugspaneler og stationsprognoser kan udnyttes til etableringen af en model....

  17. Trophoblast lineage cells derived from human induced pluripotent stem cells

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Ying, E-mail: ying.chen@hc.msu.edu [Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, 333 Bostwick NE, Grand Rapids, MI 49503 (United States); Wang, Kai; Chandramouli, Gadisetti V.R. [Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, 333 Bostwick NE, Grand Rapids, MI 49503 (United States); Knott, Jason G. [Developmental Epigenetics Laboratory, Department of Animal Science, Michigan State University (United States); Leach, Richard, E-mail: Richard.leach@hc.msu.edu [Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, 333 Bostwick NE, Grand Rapids, MI 49503 (United States); Department of Obstetrics, Gynecology and Women’s Health, Spectrum Health Medical Group (United States)

    2013-07-12

    Highlights: •Epithelial-like phenotype of trophoblast lineage cells derived from human iPS cells. •Trophoblast lineage cells derived from human iPS cells exhibit trophoblast function. •Trophoblasts from iPS cells provides a proof-of-concept in regenerative medicine. -- Abstract: Background: During implantation, the blastocyst trophectoderm attaches to the endometrial epithelium and continues to differentiate into all trophoblast subtypes, which are the major components of a placenta. Aberrant trophoblast proliferation and differentiation are associated with placental diseases. However, due to ethical and practical issues, there is almost no available cell or tissue source to study the molecular mechanism of human trophoblast differentiation, which further becomes a barrier to the study of the pathogenesis of trophoblast-associated diseases of pregnancy. In this study, our goal was to generate a proof-of-concept model for deriving trophoblast lineage cells from induced pluripotency stem (iPS) cells from human fibroblasts. In future studies the generation of trophoblast lineage cells from iPS cells established from patient’s placenta will be extremely useful for studying the pathogenesis of individual trophoblast-associated diseases and for drug testing. Methods and results: Combining iPS cell technology with BMP4 induction, we derived trophoblast lineage cells from human iPS cells. The gene expression profile of these trophoblast lineage cells was distinct from fibroblasts and iPS cells. These cells expressed markers of human trophoblasts. Furthermore, when these cells were differentiated they exhibited invasive capacity and placental hormone secretive capacity, suggesting extravillous trophoblasts and syncytiotrophoblasts. Conclusion: Trophoblast lineage cells can be successfully derived from human iPS cells, which provide a proof-of-concept tool to recapitulate pathogenesis of patient placental trophoblasts in vitro.

  18. Forebyggelse af vertikal transmission af human immundefektvirus i Danmark

    DEFF Research Database (Denmark)

    Rasmussen, Maria Birkvad; Rasmussen, Johannes Boyen; Nielsen, Vibeke Rosenfeldt

    2008-01-01

    during the study period. In 79% of the cases, the woman knew her HIV status at the beginning of her pregnancy. The median CD4 count before delivery was 447 x 10(6)/l, and in 76% of the cases the HIV-RNA was ... breastfed. None of the children were infected during pregnancy, delivery or after birth. During the same period of time, 8 children were diagnosed with HIV in Denmark; they were born to mothers whose HIV infection was not diagnosed during pregnancy or delivery and therefore preventive treatment...... was not initiated. CONCLUSION: As long as preventive treatment strategies are followed, there is no transmission of HIV from mother to child, neither during pregnancy nor during or after birth. Udgivelsesdato: 2008-Aug-18...

  19. Neurogenic differentiation of amniotic fluid stem cells.

    Science.gov (United States)

    Rosner, M; Mikula, M; Preitschopf, A; Feichtinger, M; Schipany, K; Hengstschläger, M

    2012-05-01

    In 2003, human amniotic fluid has been shown to contain stem cells expressing Oct-4, a marker for pluripotency. This finding initiated a rapidly growing and very promising new stem cell research field. Since then, amniotic fluid stem (AFS) cells have been demonstrated to harbour the potential to differentiate into any of the three germ layers and to form three-dimensional aggregates, so-called embryoid bodies, known as the principal step in the differentiation of pluripotent stem cells. Marker selection and minimal dilution approaches allow the establishment of monoclonal AFS cell lineages with high proliferation potential. AFS cells have a lower risk for tumour development and do not raise the ethical issues of embryonic stem cells. Compared to induced pluripotent stem cells, AFS cells do not need exogenic treatment to induce pluripotency, are chromosomal stable and do not harbour the epigenetic memory and accumulated somatic mutations of specific differentiated source cells. Compared to adult stem cells, AFS can be grown in larger quantities and show higher differentiation potential. Accordingly, in the recent past, AFS became increasingly accepted as an optimal tool for basic research and probably also for specific cell-based therapies. Here, we review the current knowledge on the neurogenic differentiation potential of AFS cells.

  20. Human Neural Cell-Based Biosensor

    Science.gov (United States)

    2013-05-28

    including incubation with factors such as SHH ) and proceed to Human Neural Progenitor Cells Dopaminergic Differentiation β-III Tubulin/TH...exposure in human embryonic stem cells. J Recept Signal Transduct Res. 2011 Jun;31(3):206-13. Gerwe BA, Angel PM, West FD, Hasneen K, Young A

  1. Expression of MLL-AF4 or AF4-MLL fusions does not impact the efficiency of DNA damage repair.

    Science.gov (United States)

    Castaño, Julio; Herrero, Ana B; Bursen, Aldeheid; González, Federico; Marschalek, Rolf; Gutiérrez, Norma C; Menendez, Pablo

    2016-05-24

    The most frequent rearrangement of the human MLL gene fuses MLL to AF4 resulting in high-risk infant B-cell acute lymphoblastic leukemia (B-ALL). MLL fusions are also hallmark oncogenic events in secondary acute myeloid leukemia. They are a direct consequence of mis-repaired DNA double strand breaks (DNA-DSBs) due to defects in the DNA damage response associated with exposure to topoisomerase-II poisons such as etoposide. It has been suggested that MLL fusions render cells susceptible to additional chromosomal damage upon exposure to etoposide. Conversely, the genome-wide mutational landscape in MLL-rearranged infant B-ALL has been reported silent. Thus, whether MLL fusions compromise the recognition and/or repair of DNA damage remains unanswered. Here, the fusion proteins MLL-AF4 (MA4) and AF4-MLL (A4M) were CRISPR/Cas9-genome edited in the AAVS1 locus of HEK293 cells as a model to study MLL fusion-mediated DNA-DSB formation/repair. Repair kinetics of etoposide- and ionizing radiation-induced DSBs was identical in WT, MA4- and A4M-expressing cells, as revealed by flow cytometry, by immunoblot for γH2AX and by comet assay. Accordingly, no differences were observed between WT, MA4- and A4M-expressing cells in the presence of master proteins involved in non-homologous end-joining (NHEJ; i.e.KU86, KU70), alternative-NHEJ (Alt-NHEJ; i.e.LigIIIa, WRN and PARP1), and homologous recombination (HR, i.e.RAD51). Moreover, functional assays revealed identical NHEJ and HR efficiency irrespective of the genotype. Treatment with etoposide consistently induced cell cycle arrest in S/G2/M independent of MA4/A4M expression, revealing a proper activation of the DNA damage checkpoints. Collectively, expression of MA4 or A4M does neither influence DNA signaling nor DNA-DSB repair.

  2. Sammenligning af energiforbrug i supermarkeder

    DEFF Research Database (Denmark)

    Knudsen, Hans Jørgen Høgaard

    2005-01-01

    kuldebehov som procent af det maksimale kuldebehov i hele måleperioden. For referenceanlægget er benyttet en maksimal belastning på kølesiden på 110 kW og en maksimal belastning på frostsiden på 40 kW. Endvidere er anlæggenes middel COP (forholdet mellem leveret kuldeydelse og forbrugt energi) i måleperioden...... til beregning af køleydelsen. Årsagen hertil er metodens store følsomhed over for de nominelle data. Energiforbruget beregnet på grundlag af et reference belastningsprofil er, inden for måleusikkerheden, ens for de to kaskadeanlæg og det ene af de konventionelle anlæg. De sidste konventionelle anlæg...

  3. The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms

    KAUST Repository

    Park, Hyo-Young

    2017-04-21

    The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. These proteins also move rapidly and continuously in the nuclei, and their movements are affected by ATP depletion. The U2AF65 proteins are splicing factors that interact with SF1 and U2AF35 proteins to promote U2snRNP for the recognition of the pre-mRNA 3\\' splice site during early spliceosome assembly. We have determined the subcellular localization and movement of these proteins\\' Arabidopsis homologs. It was found that Arabidopsis U2AF65 homologs, AtU2AF65a, and AtU2AF65b proteins interact with AtU2AF35a and AtU2AF35b, which are Arabidopsis U2AF35 homologs. We have examined the mobility of these proteins including AtSF1 using fluorescence recovery after photobleaching and fluorescence loss in photobleaching analyses. These proteins displayed dynamic movements in nuclei and their movements were affected by ATP depletion. We have also demonstrated that these proteins shuttle between nuclei and cytoplasms, suggesting that they may also function in cytoplasm. These results indicate that such splicing factors show very similar characteristics to their human counterparts, suggesting evolutionary conservation.

  4. Udredning af xerostomi og hyposalivation

    DEFF Research Database (Denmark)

    Pedersen, Anne Marie Lynge; Dynesen, Anja Weirsøe; Jensen, Siri Beier

    2011-01-01

    Mundtørhed kræver grundig undersøgelse Xerostomi er ofte associeret til betydelig nedsat spytsekretion, men kan forekomme, uden at der objektivt kan påvises nedsat spytsekretion. Udredning af patienter, som klager over mundtørhed, bør således altid inkludere en omhyggelig anamnese, en detaljeret ...... adækvate behandlingsstrategier for den patient, som lider af xerostomi og nedsat spytsekretion....

  5. En beskrivelse af tidligere stofmisbrugere

    DEFF Research Database (Denmark)

    Hecksher, Dorte

    2004-01-01

    Artiklen fokuserer på en del af et sample fra en større opfølgningsundersøgelse af døgnbehandlingsindsatsen overfor stofmisbrugere i Danmark. Formålet med artiklen er at beskrive den stoffri gruppe forud for indskrivning i døgnbehandling og i de første to år efter udskrivning fra døgnbehandling. ...

  6. Amniotic fluid may act as a transporting pathway for signaling molecules and stem cells during the embryonic development of amniotes.

    Science.gov (United States)

    Tong, Xinglong

    2013-11-01

    Amniotic fluid (AF) is formed at the very early stages of pregnancy, and is present throughout embryonic development of amniotes. It is well-known that AF provides a protective sac around the fetus that allows fetal movement and growth, and prevents mechanical and thermal shock. However, a growing body of evidence has shown that AF contains a number of proteins and peptides, including growth factors and cytokines, which potently affect cellular growth and proliferation. In addition, pluripotent stem cells have recently been identified in AF. Herein, this article reviews the biological properties of AF during embryonic development and speculates that AF may act as a transporting pathway for signaling molecules and stem cells during amniote embryonic development. Defining this novel function of AF is potentially significant for further understanding embryonic development and regenerative medicine, preventing genetic diseases, and developing therapeutic options for human malignancies.

  7. Cortical network from human embryonic stem cells

    OpenAIRE

    2010-01-01

    Abstract The connection of embryonic stem cell technology and developmental biology provides valuable tools to decipher the mechanisms underlying human brain development and diseases, especially among neuronal populations, that are not readily available in primary cultures. It is obviously the case of neurons forming the human cerebral cortex. In the images that are presented, the neurons were generated in vitro from human embryonic stem cells via forebrain-like progenitors. Maintained in cul...

  8. Implementering af MultifunC

    DEFF Research Database (Denmark)

    Henze-Pedersen, Sofie; Kohl, Katrine Syppli; Oldrup, Helene

    (MultifunC), som allerede havde vist lovende resultater i Norge og Sverige. I 2011 og 2012 åbnede to danske MultifunC-institutioner derfor i henholdsvis København og Aarhus. Denne rapport undersøger, hvordan implementeringen af MultifunC-programmet er forløbet i Danmark. Undersøgelsen viser, at ingen af de...... to danske institutioner lykkedes med at implementere MultifunC tilfredsstillende i løbet af implementeringsperioden. Det har fx været vanskeligt at implementere det tætte tværfaglige samarbejde mellem medarbejdere med forskellige fagprofessionelle baggrunde, som behandlingsprogrammet indebærer. Det har...... desuden været særdeles vanskeligt for de danske MultifunC-institutioner at få etableret det eksterne samarbejde med de sociale myndigheder i kommunerne, politiet, skoler og praktiksteder samt fritidstilbud i nærområdet, som også er en væsentlig del af behandlingsprogrammet. Undersøgelsen af finansieret af...

  9. Overvejelser vedr. effektvurdering af det Digitale Sygehus

    DEFF Research Database (Denmark)

    Andersen, Povl Erik Rostgård; Wit, Camilla Kølsen de

    2001-01-01

    Nærværende skrift indeholder en beskrivelse af de overvejelser og planer, der er lavet på projektet 'Effektvurdering af Det Digitale Sygehus' medio dec. 2001.......Nærværende skrift indeholder en beskrivelse af de overvejelser og planer, der er lavet på projektet 'Effektvurdering af Det Digitale Sygehus' medio dec. 2001....

  10. Dimensionering af rørisolering

    DEFF Research Database (Denmark)

    Olufsen, P.

    Meddelelsen gennemgår metoderne til dimensionering af termisk isolering af tekniske installationer i bygninger med hovedvægten på isolering af rør og beholdere i varme- og brugsvandsanlæg. Grundlaget for opstilling af mindstekrav til rørisolering diskuteres og der sammenlignes med tilsvarende...

  11. Overvejelser vedr. effektvurdering af det Digitale Sygehus

    DEFF Research Database (Denmark)

    Andersen, Povl Erik Rostgård; Wit, Camilla Kølsen de

    2001-01-01

    Nærværende skrift indeholder en beskrivelse af de overvejelser og planer, der er lavet på projektet 'Effektvurdering af Det Digitale Sygehus' medio dec. 2001.......Nærværende skrift indeholder en beskrivelse af de overvejelser og planer, der er lavet på projektet 'Effektvurdering af Det Digitale Sygehus' medio dec. 2001....

  12. Udvikling af systemleverancer, parametri og BIM

    DEFF Research Database (Denmark)

    Kortegaard, Per

    2016-01-01

    Seminaret rummede indlæg fra både nogle af projektets tidligere ph.d.-studerende og forskellige kompetente professionelle personer fra forskellige grene af praksis. Seminaret gav samlet et fint billede af status indenfor udviklingen af systemleverancer og deres anvendelse i relation til BIM...

  13. Kants Kritik af dømmekraften

    DEFF Research Database (Denmark)

    Introduktion til Kants Kritik af dømmekraften bestående af otte artikler, der følger værkets egen tematiske opbygning......Introduktion til Kants Kritik af dømmekraften bestående af otte artikler, der følger værkets egen tematiske opbygning...

  14. Demokratiseringen af den politiske diskurs i Polen

    DEFF Research Database (Denmark)

    Arnfast, Juni Søderberg

    1999-01-01

    Gennem en analyse af brugen af personlige pronominer i politiske tekster fra udvalgte, polske medier diskuteres forandringerne i den polske, politiske diskurs gennem 1980'erne. Det påvises at det kommunistiske styres anvendelse af pronominet 'vi' skifter konnotationer i retning af en mindre ideol...

  15. På sporet af de gyldne opskrifter?

    DEFF Research Database (Denmark)

    Poulfelt, Flemming

    2015-01-01

    Børsen Ledelse. Markedet for kogebøger er stort. For der produceres hvert år et hav nye af slagsen. Nogle udgives af kendte kokke, nogle af særlige eksperter inden for kost og motion, og andre af personer, der har en ægte interesse inden for gastronomi og mad. På ledelsesområdet findes en...

  16. Histone Acetyltransferase Activity of MOF Is Required for MLL-AF9 Leukemogenesis.

    Science.gov (United States)

    Valerio, Daria G; Xu, Haiming; Chen, Chun-Wei; Hoshii, Takayuki; Eisold, Meghan E; Delaney, Christopher; Cusan, Monica; Deshpande, Aniruddha J; Huang, Chun-Hao; Lujambio, Amaia; Zheng, YuJun George; Zuber, Johannes; Pandita, Tej K; Lowe, Scott W; Armstrong, Scott A

    2017-02-15

    Chromatin-based mechanisms offer therapeutic targets in acute myeloid leukemia (AML) that are of great current interest. In this study, we conducted an RNAi-based screen to identify druggable chromatin regulator-based targets in leukemias marked by oncogenic rearrangements of the MLL gene. In this manner, we discovered the H4K16 histone acetyltransferase (HAT) MOF to be important for leukemia cell growth. Conditional deletion of Mof in a mouse model of MLL-AF9-driven leukemogenesis reduced tumor burden and prolonged host survival. RNA sequencing showed an expected downregulation of genes within DNA damage repair pathways that are controlled by MOF, as correlated with a significant increase in yH2AX nuclear foci in Mof-deficient MLL-AF9 tumor cells. In parallel, Mof loss also impaired global H4K16 acetylation in the tumor cell genome. Rescue experiments with catalytically inactive mutants of MOF showed that its enzymatic activity was required to maintain cancer pathogenicity. In support of the role of MOF in sustaining H4K16 acetylation, a small-molecule inhibitor of the HAT component MYST blocked the growth of both murine and human MLL-AF9 leukemia cell lines. Furthermore, Mof inactivation suppressed leukemia development in an NUP98-HOXA9-driven AML model. Taken together, our results establish that the HAT activity of MOF is required to sustain MLL-AF9 leukemia and may be important for multiple AML subtypes. Blocking this activity is sufficient to stimulate DNA damage, offering a rationale to pursue MOF inhibitors as a targeted approach to treat MLL-rearranged leukemias. Cancer Res; 77(7); 1-10. ©2017 AACR.

  17. Improvement and biological applications of fluorescent probes for zinc, ZnAFs.

    Science.gov (United States)

    Hirano, Tomoya; Kikuchi, Kazuya; Urano, Yasuteru; Nagano, Tetsuo

    2002-06-12

    The development and cellular applications of novel fluorescent probes for Zn2+, ZnAF-1F, and ZnAF-2F are described. Fluorescein is used as a fluorophore of ZnAFs, because its excitation and emission wavelengths are in the visible range, which minimizes cell damage and autofluorescence by excitation light. N,N-Bis(2-pyridylmethyl)ethylenediamine, used as an acceptor for Zn2+, is attached directly to the benzoic acid moiety of fluorescein, resulting in very low quantum yields of 0.004 for ZnAF-1F and 0.006 for ZnAF-2F under physiological conditions (pH 7.4) due to the photoinduced electron-transfer mechanism. Upon the addition of Zn2+, the fluorescence intensity is quickly increased up to 69-fold for ZnAF-1F and 60-fold for ZnAF-2F. Apparent dissociation constants (K(d)) are in the nanomolar range, which affords sufficient sensitivity for biological applications. ZnAFs do not fluoresce in the presence of other biologically important cations such as Ca2+ and Mg2+, and are insensitive to change of pH. The complexes with Zn2+ of previously developed ZnAFs, ZnAF-1, and ZnAF-2 decrease in fluorescence intensity below pH 7.0 owing to protonation of the phenolic hydroxyl group of fluorescein, whose pKa value is 6.2. On the other hand, the Zn2+ complexes of ZnAF-1F and ZnAF-2F emit stable fluorescence around neutral and slightly acidic conditions because the pKa values are shifted to 4.9 by substitution of electron-withdrawing fluorine at the ortho position of the phenolic hydroxyl group. For application to living cells, the diacetyl derivative of ZnAF-2F, ZnAF-2F DA, was synthesized. ZnAF-2F DA can permeate through the cell membrane, and is hydrolyzed by esterase in the cytosol to yield ZnAF-2F, which is retained in the cells. Using ZnAF-2F DA, we could measure the changes of intracellular Zn2+ in cultured cells and hippocampal slices.

  18. Human hematopoietic cell culture, transduction, and analyses

    DEFF Research Database (Denmark)

    Bonde, Jesper; Wirthlin, Louisa; Kohn, Donald B;

    2008-01-01

    This unit provides methods for introducing genes into human hematopoietic progenitor cells. The Basic Protocol describes isolation of CD34(+) cells, transduction of these cells with a retroviral vector on fibronectin-coated plates, assaying the efficiency of transduction, and establishing long...

  19. Human embryonic stem cells derived by somatic cell nuclear transfer.

    Science.gov (United States)

    Tachibana, Masahito; Amato, Paula; Sparman, Michelle; Gutierrez, Nuria Marti; Tippner-Hedges, Rebecca; Ma, Hong; Kang, Eunju; Fulati, Alimujiang; Lee, Hyo-Sang; Sritanaudomchai, Hathaitip; Masterson, Keith; Larson, Janine; Eaton, Deborah; Sadler-Fredd, Karen; Battaglia, David; Lee, David; Wu, Diana; Jensen, Jeffrey; Patton, Phillip; Gokhale, Sumita; Stouffer, Richard L; Wolf, Don; Mitalipov, Shoukhrat

    2013-06-06

    Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state.

  20. Betydningen af pesticider for forekomsten af vilde bier

    DEFF Research Database (Denmark)

    Bruus, Marianne; Dupont, Yoko Luise; Grant, Ruth;

    Både i Danmark og andre steder er der observeret en nedgang i de insekter, der bestøver landmandens afgrøder og de vilde planter. Tilbagegangen falder sammen med, at landbrugsdriften er blevet mere intensiv, og anvendelsen af pesticider er øget. Vi ved imidlertid ikke, hvilken rolle pesticiderne...... vigtige for bestøvningen af fx kløver og mange vilde planter. Da humlebier og andre vilde bier har en anden adfærd og placerer deres reder andre steder end honningbierne, er det ikke givet, at de er beskyttet mod pesticideksponering, selv om honningbierne er. I pilotprojektet ”Betydningen af pesticider...... for forekomsten af vilde bier og honningbier” har Aarhus Universitet på to landbrugsbedrifter gennemført en indledende undersøgelse af, om humlebier udsættes for pesticider i danske landbrugsområder. Humlebistader blev sat ud i to værkstedsområder (ved Fensten og Rodstenseje syd for Odder), hvor driftsformen...

  1. Effekter ved udlicitering af offentlige opgaver

    DEFF Research Database (Denmark)

    Petersen, Ole Helby; Hjelmar, Ulf; Vrangbæk, Karsten;

    2014-01-01

    Denne rapport præsenterer resultaterne af en systematisk forskningsoversigt over studier af effekter af udlicitering publiceret i perioden 2011-2014. Forskningsoversigten opdaterer en AKF-rapport fra 2011, som gennemgik publicerede undersøgelser af effekter ved udlicitering i perioden 2000-2011 (......-2014. Indsamlingen og vurderingen af studier er afsluttet d. 27. oktober 2014, hvilket betyder, at studier udgivet efter denne dato ikke indgår i forskningsoversigten....

  2. Human Neuroepithelial Cells Express NMDA Receptors

    Directory of Open Access Journals (Sweden)

    Cappell B

    2003-11-01

    Full Text Available Abstract L-glutamate, an excitatory neurotransmitter, binds to both ionotropic and metabotropic glutamate receptors. In certain parts of the brain the BBB contains two normally impermeable barriers: 1 cerebral endothelial barrier and 2 cerebral epithelial barrier. Human cerebral endothelial cells express NMDA receptors; however, to date, human cerebral epithelial cells (neuroepithelial cells have not been shown to express NMDA receptor message or protein. In this study, human hypothalamic sections were examined for NMDA receptors (NMDAR expression via immunohistochemistry and murine neuroepithelial cell line (V1 were examined for NMDAR via RT-PCR and Western analysis. We found that human cerebral epithelium express protein and cultured mouse neuroepithelial cells express both mRNA and protein for the NMDA receptor. These findings may have important consequences for neuroepithelial responses during excitotoxicity and in disease.

  3. A novel method to derive amniotic fluid stem cells for therapeutic purposes

    Directory of Open Access Journals (Sweden)

    Vantanasiri Chanchai

    2010-10-01

    Full Text Available Abstract Background Human amniotic fluid stem (hAFS cells have become an attractive stem cell source for medical therapy due to both their ability to propagate as stem cells and the lack of ethical debate that comes with the use of embryonic stem cells. Although techniques to derive stem cells from amniotic fluid are available, the techniques have limitations for clinical uses, including a requirement of long periods of time for stem cell production, population heterogeneity and xeno-contamination from using animal antibody-coated magnetic beads. Herein we describe a novel isolation method that fits for hAFS derivation for cell-based therapy. Methods and Results With our method, single hAFS cells generate colonies in a primary culture of amniotic fluid cells. Individual hAFS colonies are then expanded by subculturing in order to make a clonal hAFS cell line. This method allows derivation of a substantial amount of a pure stem cell population within a short period of time. Indeed, 108 cells from a clonal hAFS line can be derived in two weeks using our method, while previous techniques require two months. The resultant hAFS cells show a 2-5 times greater proliferative ability than with previous techniques and a population doubling time of 0.8 days. The hAFS cells exhibit typical hAFS cell characteristics including the ability to differentiate into adipogenic-, osteogenic- and neurogenic lineages, expression of specific stem cell markers including Oct4, SSEA4, CD29, CD44, CD73, CD90, CD105 and CD133, and maintenance of a normal karyotype over long culture periods. Conclusions We have created a novel hAFS cell derivation method that can produce a vast amount of high quality stem cells within a short period of time. Our technique makes possibility for providing autogenic fetal stem cells and allogeneic cells for future cell-based therapy.

  4. Modeling BCR-ABL and MLL-AF9 leukemia in a human bone marrow-like scaffold-based xenograft model

    NARCIS (Netherlands)

    Sontakke, P.; Carretta, M.; Jaques, J.; Brouwers-Vos, A. Z.; Lubbers-Aalders, L.; Yuan, H.; de Bruijn, J. D.; Martens, A. C. M.; Vellenga, E.; Groen, R. W. J.; Schuringa, J. J.

    2016-01-01

    Although NOD-SCID IL2R gamma(-/-) (NSG) xenograft mice are currently the most frequently used model to study human leukemia in vivo, the absence of a human niche severely hampers faithful recapitulation of the disease. We used NSG mice in which ceramic scaffolds seeded with human mesenchymal stromal

  5. Cell Cycle Progression of Human Cells Cultured in Rotating Bioreactor

    Science.gov (United States)

    Parks, Kelsey

    2009-01-01

    Space flight has been shown to alter the astronauts immune systems. Because immune performance is complex and reflects the influence of multiple organ systems within the host, scientists sought to understand the potential impact of microgravity alone on the cellular mechanisms critical to immunity. Lymphocytes and their differentiated immature form, lymphoblasts, play an important and integral role in the body's defense system. T cells, one of the three major types of lymphocytes, play a central role in cell-mediated immunity. They can be distinguished from other lymphocyte types, such as B cells and natural killer cells by the presence of a special receptor on their cell surface called T cell receptors. Reported studies have shown that spaceflight can affect the expression of cell surface markers. Cell surface markers play an important role in the ability of cells to interact and to pass signals between different cells of the same phenotype and cells of different phenotypes. Recent evidence suggests that cell-cycle regulators are essential for T-cell function. To trigger an effective immune response, lymphocytes must proliferate. The objective of this project is to investigate the changes in growth of human cells cultured in rotating bioreactors and to measure the growth rate and the cell cycle distribution for different human cell types. Human lymphocytes and lymphoblasts will be cultured in a bioreactor to simulate aspects of microgravity. The bioreactor is a cylindrical culture vessel that incorporates the aspects of clinostatic rotation of a solid fluid body around a horizontal axis at a constant speed, and compensates gravity by rotation and places cells within the fluid body into a sustained free-fall. Cell cycle progression and cell proliferation of the lymphocytes will be measured for a number of days. In addition, RNA from the cells will be isolated for expression of genes related in cell cycle regulations.

  6. Cell Cycle Progression of Human Cells Cultured in Rotating Bioreactor

    Science.gov (United States)

    Parks, Kelsey

    2009-01-01

    Space flight has been shown to alter the astronauts immune systems. Because immune performance is complex and reflects the influence of multiple organ systems within the host, scientists sought to understand the potential impact of microgravity alone on the cellular mechanisms critical to immunity. Lymphocytes and their differentiated immature form, lymphoblasts, play an important and integral role in the body's defense system. T cells, one of the three major types of lymphocytes, play a central role in cell-mediated immunity. They can be distinguished from other lymphocyte types, such as B cells and natural killer cells by the presence of a special receptor on their cell surface called T cell receptors. Reported studies have shown that spaceflight can affect the expression of cell surface markers. Cell surface markers play an important role in the ability of cells to interact and to pass signals between different cells of the same phenotype and cells of different phenotypes. Recent evidence suggests that cell-cycle regulators are essential for T-cell function. To trigger an effective immune response, lymphocytes must proliferate. The objective of this project is to investigate the changes in growth of human cells cultured in rotating bioreactors and to measure the growth rate and the cell cycle distribution for different human cell types. Human lymphocytes and lymphoblasts will be cultured in a bioreactor to simulate aspects of microgravity. The bioreactor is a cylindrical culture vessel that incorporates the aspects of clinostatic rotation of a solid fluid body around a horizontal axis at a constant speed, and compensates gravity by rotation and places cells within the fluid body into a sustained free-fall. Cell cycle progression and cell proliferation of the lymphocytes will be measured for a number of days. In addition, RNA from the cells will be isolated for expression of genes related in cell cycle regulations.

  7. Ledelsesroller i globalisering af cleantech

    DEFF Research Database (Denmark)

    Knudsen, Poul Frøjk; Tambo, Torben

    2011-01-01

    Cleantech anses i de fleste industrialiserede lande for den nye vækstbølge, der både skal fastholde arbejdspladser og beskæftigelse og også redde miljøet. Danmark har været early-mover på vindkraft. Aktiv ledelse har til dato fastholdt et forspring. Denne aktive ledelse har sikret anvendelse af...... nyeste teknologier, globalisering af salg, installation og produktion, og at vækstdagsordenen er blevet fastholdt. Den globale satsning på cleantech sætter disse ledelsesmæssige kvaliteter under pres. Dette studie ser på en mellemstor komponentleverandør til vindindustrien, og dennes kamp...... økonomisk bæredygtighed præger ledelsesprocesserne. Studiet giver et sæt anbefalinger til en justering af egen-forståelsen i den bredere del af cleantech-sektoren med fokus på ledelse, som i denne sektor i særlig grad skal håndtere krav ud over den klassiske supply chain i form af politiske dagsordener i...

  8. Derivation of naive human embryonic stem cells.

    Science.gov (United States)

    Ware, Carol B; Nelson, Angelique M; Mecham, Brigham; Hesson, Jennifer; Zhou, Wenyu; Jonlin, Erica C; Jimenez-Caliani, Antonio J; Deng, Xinxian; Cavanaugh, Christopher; Cook, Savannah; Tesar, Paul J; Okada, Jeffrey; Margaretha, Lilyana; Sperber, Henrik; Choi, Michael; Blau, C Anthony; Treuting, Piper M; Hawkins, R David; Cirulli, Vincenzo; Ruohola-Baker, Hannele

    2014-03-25

    The naïve pluripotent state has been shown in mice to lead to broad and more robust developmental potential relative to primed mouse epiblast cells. The human naïve ES cell state has eluded derivation without the use of transgenes, and forced expression of OCT4, KLF4, and KLF2 allows maintenance of human cells in a naïve state [Hanna J, et al. (2010) Proc Natl Acad Sci USA 107(20):9222-9227]. We describe two routes to generate nontransgenic naïve human ES cells (hESCs). The first is by reverse toggling of preexisting primed hESC lines by preculture in the histone deacetylase inhibitors butyrate and suberoylanilide hydroxamic acid, followed by culture in MEK/ERK and GSK3 inhibitors (2i) with FGF2. The second route is by direct derivation from a human embryo in 2i with FGF2. We show that human naïve cells meet mouse criteria for the naïve state by growth characteristics, antibody labeling profile, gene expression, X-inactivation profile, mitochondrial morphology, microRNA profile and development in the context of teratomas. hESCs can exist in a naïve state without the need for transgenes. Direct derivation is an elusive, but attainable, process, leading to cells at the earliest stage of in vitro pluripotency described for humans. Reverse toggling of primed cells to naïve is efficient and reproducible.

  9. Brug af alternativ isolering i forbindelse med renovering af ældre etageejendomme

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place

    2003-01-01

    Resume af rapport om praktiske erfaringer med brug af alternative isoleringsmaterialer i en renoveringsopgave, udarbejdet af Byfornyelse København m.fl. under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"...

  10. Regulatory T Cells in Human Ovarian Cancer

    Directory of Open Access Journals (Sweden)

    Dong-Jun Peng

    2012-01-01

    Full Text Available Multiple layers of suppressive components including regulatory T (TReg cells, suppressive antigen-presenting cells, and inhibitory cytokines form suppressive networks in the ovarian cancer microenvironment. It has been demonstrated that as a major suppressive element, TReg cells infiltrate tumor, interact with several types of immune cells, and mediate immune suppression through different molecular and cellular mechanisms. In this paper, we focus on human ovarian cancer and will discuss the nature of TReg cells including their subsets, trafficking, expansion, and function. We will briefly review the development of manipulation of TReg cells in preclinical and clinical settings.

  11. Generation of pancreatic islet cells from human embryonic stem cells

    Institute of Scientific and Technical Information of China (English)

    ZHANG DongHui; JIANG Wei; SHI Yan; DENG HongKui

    2009-01-01

    Efficiently obtaining functional pancreaUc islet cells derived from human embryonic stem (hES) cells not only provides great potential to solve the shortage of islets sources for type I diabetes cell therapy,but also benefits the study of the development of the human pancreas and diabetes pathology. In 2001,hES cells were reported to have the capacity to generate insulin-producing cells by spontaneous differentiation in vitro. Since then, many strategies (such as overexpression of key transcription factors,delivery of key proteins for pancreatic development, co-transplantation of differentiated hES cells along with fetal pancreas, stepwise differentiation by mimicking in vivo pancreatic development) have been employed in order to induce the differentiation of pancreatic islet cells from hES cells. Moreover, patient-specific induced pluripotent stem (iPS) cells can be generated by reprogramming somatic cells.iPS cells have characteristics similar to those of ES cells and offer a new cell source for type I diabetes cell therapy that reduces the risk of immunologic rejection. In this review, we summarize the recent progress made in the differentiation of hES and iPS cells into functional pancreatic islet cells and discuss the challenges for their future study.

  12. Generation of pancreatic islet cells from human embryonic stem cells

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Efficiently obtaining functional pancreatic islet cells derived from human embryonic stem(hES) cells not only provides great potential to solve the shortage of islets sources for type I diabetes cell therapy,but also benefits the study of the development of the human pancreas and diabetes pathology.In 2001,hES cells were reported to have the capacity to generate insulin-producing cells by spontaneous differentiation in vitro.Since then,many strategies(such as overexpression of key transcription factors,delivery of key proteins for pancreatic development,co-transplantation of differentiated hES cells along with fetal pancreas,stepwise differentiation by mimicking in vivo pancreatic development) have been employed in order to induce the differentiation of pancreatic islet cells from hES cells.Moreover,patient-specific induced pluripotent stem(iPS) cells can be generated by reprogramming somatic cells.iPS cells have characteristics similar to those of ES cells and offer a new cell source for type I diabetes cell therapy that reduces the risk of immunologic rejection.In this review,we summarize the recent progress made in the differentiation of hES and iPS cells into functional pancreatic islet cells and discuss the challenges for their future study.

  13. Plasma membrane proteomics of human embryonic stem cells and human embryonal carcinoma cells.

    NARCIS (Netherlands)

    Dormeyer, W.; van Hoof, D.; Braam, S.R.; Heck, A.J.R.; Mummery, C.L.; Krijgsveld, J.

    2008-01-01

    Human embryonic stem cells (hESCs) are of immense interest in regenerative medicine as they can self-renew indefinitely and can give rise to any adult cell type. Human embryonal carcinoma cells (hECCs) are the malignant counterparts of hESCs found in testis tumors. hESCs that have acquired chromosom

  14. Den gode facilitator af refleksionsarbejde

    DEFF Research Database (Denmark)

    Jørgensen, Pia

    2009-01-01

    tværfaglig lektorgruppe fra social og sundhedssektoren.’Learning by doing’, selvevaluering og sparring følger herefter som bud på, hvordan man kan leve op til de tilsyneladende ret utopiske krav til en god facilitator. At kunne skabe det tillidsfulde refleksionsrum og at kunne stille gode...... præsenteres i det følgende afsnit, og forfatteren argumenterer for begrebet facilitator af refleksionsarbejde. Herefter udfoldes rollen som facilitator ifølge Ghay og Lillyman. De har fokus på positive praksisoplevelser og tillidsfulde relationer. Gillie Boltons teoretiske og praktiske referenceramme...... for facilitatorrollen beskrives herefter. Bolton beskriver refleksionsarbejde som en fysisk (ikke ren kognitiv), passioneret (ikke ren intellektuel) kontekstbunden kunstnerisk proces, som kræver flair, stil og intuition. I de følgende afsnit beskrives den gode facilitator af refleksionsarbejde detaljeret af en...

  15. Reduktionen af satsen for selskabsskat gør en ændring af creditreglerne aktuel

    DEFF Research Database (Denmark)

    Rønfeldt, Thomas; Werlauff, Erik

    2013-01-01

    Artiklen analyserer sammenhængen mellem satsen for selskabsskatten og maksimeringen af creditlempelsen ved aktivitet i udlandet. Der argumenteres for, at en nedsættelse af selskabsskatten aktualoiserer behovet for en gfennemgribende reform af reglerne om maksimeret credit.......Artiklen analyserer sammenhængen mellem satsen for selskabsskatten og maksimeringen af creditlempelsen ved aktivitet i udlandet. Der argumenteres for, at en nedsættelse af selskabsskatten aktualoiserer behovet for en gfennemgribende reform af reglerne om maksimeret credit....

  16. Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells.

    Science.gov (United States)

    Unger, Christian; Felldin, Ulrika; Rodin, Sergey; Nordenskjöld, Agneta; Dilber, Sirac; Hovatta, Outi

    2016-02-03

    After the first derivations of human embryonic stem cell (hESC) lines on fetal mouse feeder cell layers, the idea of using human cells instead of mouse cells as feeder cells soon arose. Mouse cells bear a risk of microbial contamination, and nonhuman immunogenic proteins are absorbed from the feeders to hESCs. Human skin fibroblasts can be effectively used as feeder cells for hESCs. The same primary cell line, which can be safely used for up to 15 passages after stock preparations, can be expanded and used for large numbers of hESC derivations and cultures. These cells are relatively easy to handle and maintain. No animal facilities or animal work is needed. Here, we describe the derivation, culture, and cryopreservation procedures for research-grade human skin fibroblast lines. We also describe how to make feeder layers for hESCs using these fibroblasts.

  17. Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth

    Energy Technology Data Exchange (ETDEWEB)

    Varga, Nora [Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, Budapest (Hungary); Vereb, Zoltan; Rajnavoelgyi, Eva [Department of Immunology, Medical and Health Science Centre, University of Debrecen, Debrecen (Hungary); Nemet, Katalin; Uher, Ferenc; Sarkadi, Balazs [Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, Budapest (Hungary); Apati, Agota, E-mail: apati@kkk.org.hu [Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, Budapest (Hungary)

    2011-10-28

    Highlights: Black-Right-Pointing-Pointer MSC like cells were derived from hESC by a simple and reproducible method. Black-Right-Pointing-Pointer Differentiation and immunosuppressive features of MSCl cells were similar to bmMSC. Black-Right-Pointing-Pointer MSCl cells as feeder cells support the undifferentiated growth of hESC. -- Abstract: Mesenchymal stem cell like (MSCl) cells were generated from human embryonic stem cells (hESC) through embryoid body formation, and isolated by adherence to plastic surface. MSCl cell lines could be propagated without changes in morphological or functional characteristics for more than 15 passages. These cells, as well as their fluorescent protein expressing stable derivatives, efficiently supported the growth of undifferentiated human embryonic stem cells as feeder cells. The MSCl cells did not express the embryonic (Oct4, Nanog, ABCG2, PODXL, or SSEA4), or hematopoietic (CD34, CD45, CD14, CD133, HLA-DR) stem cell markers, while were positive for the characteristic cell surface markers of MSCs (CD44, CD73, CD90, CD105). MSCl cells could be differentiated toward osteogenic, chondrogenic or adipogenic directions and exhibited significant inhibition of mitogen-activated lymphocyte proliferation, and thus presented immunosuppressive features. We suggest that cultured MSCl cells can properly model human MSCs and be applied as efficient feeders in hESC cultures.

  18. Styring af international transfer pricing

    DEFF Research Database (Denmark)

    Reuther, Peter; Rossing, Christian Plesner

    2011-01-01

    Nærværende artikel introducerer international transfer pricing, dels som et skatteretligt fænomen og dels som et værktøj til planlægning og økonomisk styring af koncernforbundne selskaber i en multinational virksomhed (MNV). Med udgangspunkt i en case-analyse af MNV’en gives der et konkret eksempel...... international transfer pricing i betragtning, når økonomiske styringssystemer i en MNV skal anvendes til planlægning og opfølgning....

  19. Styring af international transfer pricing

    DEFF Research Database (Denmark)

    Reuther, Peter; Rossing, Christian Plesner

    2011-01-01

    Nærværende artikel introducerer international transfer pricing, dels som et skatteretligt fænomen og dels som et værktøj til planlægning og økonomisk styring af koncernforbundne selskaber i en multinational virksomhed (MNV). Med udgangspunkt i en case-analyse af MNV’en gives der et konkret eksempel...... international transfer pricing i betragtning, når økonomiske styringssystemer i en MNV skal anvendes til planlægning og opfølgning....

  20. SCREENING OF DRUG RESISTANCE-RELATED GENES FROM HUMAN OVARIAN CANCER CELL LINE OC3/ADR BY DD-PCR

    Institute of Scientific and Technical Information of China (English)

    田方; 程国均; 周海胜; 王宏; 肖凤君

    2001-01-01

    Objective: To screen novel genes related to adriamycin (Adr) resistance from human ovarian cancer resistance cell line OC3/Adr. Methods: Multidrug resistant ovarian cancer cell line OC3/Adr was induced by intermittent treatment of the human parent cell line OC3 with high concentration Adr. The difference of gene expression was screened by using different display analysis to the acquired Adr-resistance subline OC3/Adr and its parent cell line OC3. Results: OC3/Adr cell line was obtained which was more resistance to Adr than the parent cell line OC3 with the resistance index (RI) of 15.4. The OC3/Adr cell line also showed cross-resistance to other anti-cancer drugs (VP16, CDDP,5FU ). It grew slowly and exhibited changes of cell cycle. A number of differentially expressed ESTs (Expressed Sequence Tags, ESTs) were identified at mRNA level between the OC3/Adr and OC3. Four of 18 different ESTs were sequenced. The 431/432 base pair S1 was homologous to human sperm zona pellucida binding protein, while the other two ESTs, S3 and S4, were new gene segments, which were registered to GenBank with the number of AF 117656 and AF 126507 respectively. Particularly, the expression of S2 sequence increased in all the drug-resistance cell lines and S3 sequence overexpressed in human ovarian cancer tissues as compared with benign ovarian tumors. Adr in ovarian cancer OC3/Adr is involved with changes of multiple gene expressions.

  1. Undersøgelse af kommunernes administration af bygningsreglementets elevatorkrav

    DEFF Research Database (Denmark)

    Grangaard, Sidse

    Denne rapport undersøger kommunernes praksis omkring administrationen af bygningsreglementets krav om elevator ved nybyggeri og ombygning. Blandt de 26 kommuner, der har deltaget i undersøgelsen, var der generelt konsensus om at stille krav om elevator ved nybyggeri. Kommunerne modtager kun meget...

  2. Elektrokemisk fjernelse af cadmium fra bioasker

    DEFF Research Database (Denmark)

    Pedersen, Anne Juul; Ottosen, Lisbeth M.; Simonsen, Peter

    Posteren viser resultater fra elektrokemiske rensningsforsøg på bioasker udført i forbindelse med PSO projekt 5205 "Elektrokemisk fjernelse af cadmium fra bioasker i pilotskala og vurdering af mulighederne for nyttiggørelse af behandlet aske i beton".......Posteren viser resultater fra elektrokemiske rensningsforsøg på bioasker udført i forbindelse med PSO projekt 5205 "Elektrokemisk fjernelse af cadmium fra bioasker i pilotskala og vurdering af mulighederne for nyttiggørelse af behandlet aske i beton"....

  3. På sporet af den tabte sans

    DEFF Research Database (Denmark)

    Nielsen, Thomas Rosendal

    2014-01-01

    Analyse af en workshop om interaktive strategier i børneteater for børn. Workshoppen fandt sted på Dramatikkens hus i Oslo og blev ledet af Sara Topsøe-Jensen, kunstneriske leder af teatret Carte Blanche.......Analyse af en workshop om interaktive strategier i børneteater for børn. Workshoppen fandt sted på Dramatikkens hus i Oslo og blev ledet af Sara Topsøe-Jensen, kunstneriske leder af teatret Carte Blanche....

  4. Fiktionen, der var ude af sig selv

    DEFF Research Database (Denmark)

    Hansen, Kim Toft

    2007-01-01

    På baggrund af en analyse af Lars Frosts roman Smukke biler efter krigen (2004) reflekterer jeg over fiktionen som på én gang selvberoende og selvnedbrydende størrelse, og der henvises til Adornos æstetik som en mulig teoretisk ramme for begribelsen af dette tilsyneladende paradoks.......På baggrund af en analyse af Lars Frosts roman Smukke biler efter krigen (2004) reflekterer jeg over fiktionen som på én gang selvberoende og selvnedbrydende størrelse, og der henvises til Adornos æstetik som en mulig teoretisk ramme for begribelsen af dette tilsyneladende paradoks....

  5. Stem cells in the human breast

    DEFF Research Database (Denmark)

    Petersen, Ole William; Polyak, Kornelia

    2010-01-01

    The origins of the epithelial cells participating in the development, tissue homeostasis, and cancer of the human breast are poorly understood. However, emerging evidence suggests a role for adult tissue-specific stem cells in these processes. In a hierarchical manner, these generate the two main...

  6. Human hair genealogies and stem cell latency

    Directory of Open Access Journals (Sweden)

    Tavaré Simon

    2006-02-01

    Full Text Available Abstract Background Stem cells divide to reproduce themselves and produce differentiated progeny. A fundamental problem in human biology has been the inability to measure how often stem cells divide. Although it is impossible to observe every division directly, one method for counting divisions is to count replication errors; the greater the number of divisions, the greater the numbers of errors. Stem cells with more divisions should produce progeny with more replication errors. Methods To test this approach, epigenetic errors (methylation in CpG-rich molecular clocks were measured from human hairs. Hairs exhibit growth and replacement cycles and "new" hairs physically reappear even on "old" heads. Errors may accumulate in long-lived stem cells, or in their differentiated progeny that are eventually shed. Results Average hair errors increased until two years of age, and then were constant despite decades of replacement, consistent with new hairs arising from infrequently dividing bulge stem cells. Errors were significantly more frequent in longer hairs, consistent with long-lived but eventually shed mitotic follicle cells. Conclusion Constant average hair methylation regardless of age contrasts with the age-related methylation observed in human intestine, suggesting that error accumulation and therefore stem cell latency differs among tissues. Epigenetic molecular clocks imply similar mitotic ages for hairs on young and old human heads, consistent with a restart with each new hair, and with genealogies surreptitiously written within somatic cell genomes.

  7. Human neutrophils facilitate tumor cell transendothelial migration.

    LENUS (Irish Health Repository)

    Wu, Q D

    2012-02-03

    Tumor cell extravasation plays a key role in tumor metastasis. However, the precise mechanisms by which tumor cells migrate through normal vascular endothelium remain unclear. In this study, using an in vitro transendothelial migration model, we show that human polymorphonuclear neutrophils (PMN) assist the human breast tumor cell line MDA-MB-231 to cross the endothelial barrier. We found that tumor-conditioned medium (TCM) downregulated PMN cytocidal function, delayed PMN apoptosis, and concomitantly upregulated PMN adhesion molecule expression. These PMN treated with TCM attached to tumor cells and facilitated tumor cell migration through different endothelial monolayers. In contrast, MDA-MB-231 cells alone did not transmigrate. FACScan analysis revealed that these tumor cells expressed high levels of intercellular adhesion molecule-1 (ICAM-1) but did not express CD11a, CD11b, or CD18. Blockage of CD11b and CD18 on PMN and of ICAM-1 on MDA-MB-231 cells significantly attenuated TCM-treated, PMN-mediated tumor cell migration. These tumor cells still possessed the ability to proliferate after PMN-assisted transmigration. These results indicate that TCM-treated PMN may serve as a carrier to assist tumor cell transendothelial migration and suggest that tumor cells can exploit PMN and alter their function to facilitate their extravasation.

  8. Kardiovaskulaere manifestationer af hypotyreoidisme

    DEFF Research Database (Denmark)

    Nyrop, M; Bjørnholm, K I; Nielsen, F E;

    1991-01-01

    different enzymes (including creatine kinase (CK), CK-MB and LDH) may be raised in myxoedema. The reason for this is perhaps compromized membrane function in the skeletal muscle cells. The diagnosis of myocardial infarction in myoedema requires that CK-MB constitutes at least 6% of the total CK...

  9. Af materialitet er du kommet

    DEFF Research Database (Denmark)

    Skiveren, Tobias; Gregersen, Martin

    2015-01-01

    På baggrund af bogen "Den materielle drejning. En (ny) optik i og på aktuel dansk litteratur", der udkommer i 2016, skitserer artiklen, hvordan en filosofisk-teoretisk nyorientering under overskriften "den materielle drejning" i øjeblikket udkrystalliserer sig på en henholdsvis økokritisk, posthu......, posthuman og kropsmaterialistisk strømning i dansk samtidslitteratur....

  10. Reformering af vejforvaltning i Afrika

    DEFF Research Database (Denmark)

    Kristiansen, Jørgen

    2004-01-01

    I 1980´erne skete der et hurtigt forfald af vejnettet i mange lande i Afrika syd for Sahara. Både Verdensbanken, EU og de bilaterale donorer blev klar over, at nye institutionelle løsninger var nødvendige, hvis udviklingen skulle vendes. I 1995 blev der under det såkaldte "Road Management...

  11. Reduktion af varmebehovet i kontorbyggeri

    DEFF Research Database (Denmark)

    Aggerholm, Søren

    I rapporten beskrives et projekt, der undersøger muligheden for at opnå yderligere markant reduktion af varmeforbruget i nyt kontorbyggeri, uden at indeklimaet forringes. Undersøgelserne er foretaget for tre nye kontorbygninger. I bygningerne er der målt og registreret energiforbrug, temperatur- ...

  12. På sporet af fremtidens konkurrenceevne

    DEFF Research Database (Denmark)

    Boje Rasmussen, Martin Møller

    2013-01-01

    Michael Porter og Harvard Business School står bag nyt banebrydende initiativ, Social Progress Index, som måler et samfunds evne til at skabe livskvalitet for borgerne. Dette aktualiserer debatten om udviklingen af helt nye konkurrencemodeller. Der er tale om et valg mellem tre forskellige typer...

  13. Udredning af xerostomi og hyposalivation

    DEFF Research Database (Denmark)

    Pedersen, Anne Marie Lynge; Dynesen, Anja Weirsøe; Jensen, Siri Beier

    2011-01-01

    Mundtørhed kræver grundig undersøgelse Xerostomi er ofte associeret til betydelig nedsat spytsekretion, men kan forekomme, uden at der objektivt kan påvises nedsat spytsekretion. Udredning af patienter, som klager over mundtørhed, bør således altid inkludere en omhyggelig anamnese, en detaljeret ...

  14. Digitalisering af det eksisterende byggeri

    DEFF Research Database (Denmark)

    Sørensen, Nils Lykke; Øien, Turid Borgestrand

    2010-01-01

    Digitalisering af det eksisterende byggeri er en vanskelig og uendelig opgave. Det giver kun mening at digitalisere eksisterende byggeri, når man har et konkret behov, og da kun at indhente og lagre de informationer, som man strategisk eller konkret har behov for. Erhvervs og Byggestyrelsen har i...

  15. Bliver seksualitetsforskningen lykkelig af moralismeanklager?

    DEFF Research Database (Denmark)

    Frederiksen, Maja Bissenbakker

    2010-01-01

    Anmeldelse: ‘Piger bliver ikke lykkelige af analsex’ er underholdende og sine steder forstemmende læsning om de problematiske måder, hvorpå offentlige debatter de seneste år har gjort seksualitet til genstand for en overvældende bekymring og deraf følgende krav om regulering og kontrol"...

  16. Fluoridering af drikkevandet i Danmark?

    DEFF Research Database (Denmark)

    Arvin, Erik; Spliid, Henrik; Bruvo, M.;

    2010-01-01

    Første gang sammenhængen mellem caries (huller i tænderne) og fluoridindholdet i drikkevand blev diskuteret i vandværkskredse i Danmark var ved årsmødet i 1958. Her blev det oplyst, at fluorid i drikkevand beskytter mod caries, men man konstaterede også, at der nok ikke var stemning for fluorid......-tilsætning til drikkevandet (fluoridering). Siden da er emnet ikke taget op. Formålet med dette indlæg er at besvare spørgsmålene: 1. Er der behov for fluoridering af drikkevandet i Danmark? 2. Er der alternativer? Hvordan påvirker blødgøring af vand og andre vandbehandlingsmetoder dental caries hos børn og unge......? Som baggrund herfor vil vi redegøre for resultaterne af et netop afsluttet studium af sammenhængen mellem caries og drikkevandets sammensætning, specielt m.h.t. luoridindholdet og vandets calciumindhold (hårdhed). Undersøgelsen bekræfter, at fluorid i drikkevand i væsentlig grad beskytter mod caries...

  17. Udvikling af ledelse via ledelseskoncepter

    DEFF Research Database (Denmark)

    Frimann, Søren; Thomassen, Anja Overgaard; Keller, Hanne Dauer

    I denne artikel beskæftiger vi os med, hvordan ledelse kan udvikles i spændingsfeltet mellem udefrakommende krav om mere fælles fodslag i ledelsen og den enkelte leders behov for at tilpasse sin ledelsesstil til den aktuelle kontekst. Konkret ser vi nærmere på, hvordan der blandt en gruppe af led...

  18. Organisering og styring af byggeprocessen

    DEFF Research Database (Denmark)

    Wandahl, Søren; Bohnstedt, Kristian Ditlev; Rasmussen, Arne P.

    2017-01-01

    Hermed foreligger en ny udgave af Anlægsteknik 2, hvor der er en del ændringer i forhold til 3. udgave. Dette skyldes at fagområdet Anlægsteknik er under konstant udvikling, og nye relevante emner derfor er medtaget i denne udgave samt at andre forældede emner er fjernet. Kapitel 1 omkring byggep...

  19. Virksomhedernes opfattelse af arbejdsmarkedets "restgrupper"

    DEFF Research Database (Denmark)

    Larsen, Christian Albrekt

    2010-01-01

    Artiklen beskriver, hvorledes danske arbejdsgivere opfatter ældre ledige, ledige indvandrere og ledige med lang forudgående ledighed. Det teoretiske udgangspunkt er, at arbejdsmarkedets fundamentale informationsproblemer fører til brug af statistisk diskrimination og frygt for «lemons». Analyserne...

  20. Domesticeringen af Ford i Danmark

    DEFF Research Database (Denmark)

    Wagner, Michael

    2011-01-01

    Det amerikanske automobilfirma Ford Motor Company (1903) med hovedkvarter i Detroit begyndte at drive forretning i Danmark omkring 1906. Dermed havde Ford tidligt fået et stærkt fodfæste i Nordeuropa, som udviklingen i salget af Ford automobiler de næste årtier til fulde skulle demonstrere...

  1. Signalement af en tabers temperament

    DEFF Research Database (Denmark)

    Holm, Claus

    2009-01-01

    En moderne taber tager ting for tungt, benytter sig ikke nok af glemslens nådegave og har vanskeligt ved at finde en lykkelig fortælling om fortiden. Derfor siger vi i dag: Tag en slapper, tag en pille og tag nogle samtaler....

  2. INTERACTIONS BETWEEN THE HUMAN GASTRIC CARCINOMA CELL AND THE HUMAN VASCULAR ENDOTHELIAL CELL

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective To definite the interactions between the human gastric carcinoma cell and the human vascular endothelial cell during the establishment and maintenance of the tumor vascular system and the tumor hematogenous metastasis.Methods We prepared the conditioned mediums of each cell so as to study the effect of the conditioned medium on itself or others by MTT colorimetry. The comprehensive effect of interactions between two cells was determined by stratified transfilter co-culture or direct contact co-culture.Results The conditioned medium of human gastric carcinoma cell can stimulate the proliferation of the human vascular endothelial cell, but the CM of HVEC can inhibit the growth of HGCC. Both kinds of cells can inhibit the growth of itself. The ultimate comprehensive effect of the interactions between two kinds of cells was increase of total cell numbers.Conclusion There exist the complicated interactions between the human gastric carcinoma cell and the human vascular endothelial cell during the tumor angiogenesis and the tumor hematogenous metastasis. The ultimate comprehensive effect of the interactions is increase of total cells numbers and tumor volume.

  3. Fibronectin production by human mammary cells

    Energy Technology Data Exchange (ETDEWEB)

    Stampfer, M.R. (Univ. of California, Berkeley); Vlodavsky, I.; Smith, H.S.; Ford, R.; Becker, F.F.; Riggs, J.

    1981-01-01

    Human mammary cells were examined for the presence of the high-molecular-weight surface glycoprotein fibronectin. Early passage mammary epithelial cell and fibroblast cultures from both carcinomas and normal tissues were tested for the presence of cell-associated fibronectin by immunofluorescence microscopy and for the synthesis and secretion of fibronectin by specific immunoprecipitation of metabolically labeled protein. In vivo frozen sections of primary carcinomas and normal tissues were tested for the localization of fibronectin by immunofluorescence microscopy. In contrast to the extensive fibrillar networks of fibronectin found in the fibroblast cultures, the epithelial cell cultures from both tissue sources displayed a pattern of cell-associated fibronectin characterizd by powdery, punctate staining. However, the cultured epithelial cells, as well as the fibroblasts, secreted large quantities of fibronectin into the medium. Putative myoepithelial cells also displayed extensive fibrillar networks of fibronectin. The difference in cell-associated fibronectin distribution between the epithelial cells and the fibroblasts and putative myoepithelial cells provided a simple means of quantitating stromal and myoepithelial cell contamination of the mammary epithelial cells in culture. In vivo, normal tissues showed fibronectin primarily localized in the basement membrane surrounding the epithelial cells and in the stroma. Most primary carcinomas displayed powdery, punctate staining on the epithelial cells in addition to the fibronectin present in the surrounding stroma.

  4. Æstetisk tolkning af moderne landbrugslandskaber?

    DEFF Research Database (Denmark)

    Krarup, Jonna Majgaard

    2001-01-01

    Præsentation af del af igangværende ph.d.projekt, titel: landbrugeren som kulturlandskabsforvalter - AAA, DK. I projektet arbejdes der udfra en tese om naturopfattelse som af instrumentiel og formdannende karakter. Det moderne landbrugslandskabs ses således som fysisk og æstetisk udtryk for abstr......Præsentation af del af igangværende ph.d.projekt, titel: landbrugeren som kulturlandskabsforvalter - AAA, DK. I projektet arbejdes der udfra en tese om naturopfattelse som af instrumentiel og formdannende karakter. Det moderne landbrugslandskabs ses således som fysisk og æstetisk udtryk...... for abstrakte og kulturelt definerede holdninger af forholdet mellem menneske og natur - forvaltet af den moderne landbruger. I artiklen fokuseres der på et enkelt landskabselement og dets iboende muligheder for æstetisk erfaring og fortolkning....

  5. Case-Studie af Fredericia Battle 1849

    DEFF Research Database (Denmark)

    Konzack, Lars

    2015-01-01

    En gennemgang af hvordan oplevelsesøkomien fungerer diskursivt - med Fredericia Battle 1849 som case.......En gennemgang af hvordan oplevelsesøkomien fungerer diskursivt - med Fredericia Battle 1849 som case....

  6. Politisk afmagt og udhuling af frihed

    DEFF Research Database (Denmark)

    Thomsen, Steen

    2015-01-01

    Vi oplever lige nu en politisk handlingslammelse som forudset af den liberale økonom von Hayek.......Vi oplever lige nu en politisk handlingslammelse som forudset af den liberale økonom von Hayek....

  7. Laser printing of skin cells and human stem cells.

    Science.gov (United States)

    Koch, Lothar; Kuhn, Stefanie; Sorg, Heiko; Gruene, Martin; Schlie, Sabrina; Gaebel, Ralf; Polchow, Bianca; Reimers, Kerstin; Stoelting, Stephanie; Ma, Nan; Vogt, Peter M; Steinhoff, Gustav; Chichkov, Boris

    2010-10-01

    Laser printing based on laser-induced forward transfer (LIFT) is a new biofabrication technique for the arrangement of biological materials or living cells in well-defined patterns. In the current study, skin cell lines (fibroblasts/keratinocytes) and human mesenchymal stem cells (hMSC) were chosen for laser printing experiments due to their high potential in regeneration of human skin and new application possibilities of stem cell therapy. To evaluate the influence of LIFT on the cells, their survival rate, their proliferation and apoptotic activity, and the DNA damages and modifications of their cell surface markers were assessed and statistically evaluated over several days. The cells survived the transfer procedure with a rate of 98%  +/- 1% standard error of the mean (skin cells) and 90%  +/- 10% (hMSC), respectively. All used cell types maintain their ability to proliferate after LIFT. Further, skin cells and hMSC did not show an increase of apoptosis or DNA fragmentation. In addition, the hMSC keep their phenotype as proven by fluorescence activated cell sorting (FACS) analysis. This study demonstrates LIFT as a suitable technique for unharmed computer-controlled positioning of different cell types and a promising tool for future applications in the ex vivo generation of tissue replacements.

  8. Human spleen and red blood cells

    Science.gov (United States)

    Pivkin, Igor; Peng, Zhangli; Karniadakis, George; Buffet, Pierre; Dao, Ming

    2016-11-01

    Spleen plays multiple roles in the human body. Among them is removal of old and altered red blood cells (RBCs), which is done by filtering cells through the endothelial slits, small micron-sized openings. There is currently no experimental technique available that allows us to observe RBC passage through the slits. It was previously noticed that people without a spleen have less deformable red blood cells, indicating that the spleen may play a role in defining the size and shape of red blood cells. We used detailed RBC model implemented within the Dissipative Particle Dynamics (DPD) simulation framework to study the filter function of the spleen. Our results demonstrate that spleen indeed plays major role in defining the size and shape of the healthy human red blood cells.

  9. Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus.

    Directory of Open Access Journals (Sweden)

    Nansalmaa Amarsaikhan

    Full Text Available The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals. The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease. We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates. Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased. Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293. Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.

  10. Rotating cell culture systems for human cell culture: human trophoblast cells as a model.

    Science.gov (United States)

    Zwezdaryk, Kevin J; Warner, Jessica A; Machado, Heather L; Morris, Cindy A; Höner zu Bentrup, Kerstin

    2012-01-18

    The field of human trophoblast research aids in understanding the complex environment established during placentation. Due to the nature of these studies, human in vivo experimentation is impossible. A combination of primary cultures, explant cultures and trophoblast cell lines support our understanding of invasion of the uterine wall and remodeling of uterine spiral arteries by extravillous trophoblast cells (EVTs), which is required for successful establishment of pregnancy. Despite the wealth of knowledge gleaned from such models, it is accepted that in vitro cell culture models using EVT-like cell lines display altered cellular properties when compared to their in vivo counterparts. Cells cultured in the rotating cell culture system (RCCS) display morphological, phenotypic, and functional properties of EVT-like cell lines that more closely mimic differentiating in utero EVTs, with increased expression of genes mediating invasion (e.g. matrix metalloproteinases (MMPs)) and trophoblast differentiation. The Saint Georges Hospital Placental cell Line-4 (SGHPL-4) (kindly donated by Dr. Guy Whitley and Dr. Judith Cartwright) is an EVT-like cell line that was used for testing in the RCCS. The design of the RCCS culture vessel is based on the principle that organs and tissues function in a three-dimensional (3-D) environment. Due to the dynamic culture conditions in the vessel, including conditions of physiologically relevant shear, cells grown in three dimensions form aggregates based on natural cellular affinities and differentiate into organotypic tissue-like assemblies. The maintenance of a fluid orbit provides a low-shear, low-turbulence environment similar to conditions found in vivo. Sedimentation of the cultured cells is countered by adjusting the rotation speed of the RCCS to ensure a constant free-fall of cells. Gas exchange occurs through a permeable hydrophobic membrane located on the back of the bioreactor. Like their parental tissue in vivo, RCCS

  11. Autophagy in human embryonic stem cells.

    Directory of Open Access Journals (Sweden)

    Thien Tra

    Full Text Available Autophagy (macroautophagy is a degradative process that involves the sequestration of cytosolic material including organelles into double membrane vesicles termed autophagosomes for delivery to the lysosome. Autophagy is essential for preimplantation development of mouse embryos and cavitation of embryoid bodies. The precise roles of autophagy during early human embryonic development, remain however largely uncharacterized. Since human embryonic stem cells constitute a unique model system to study early human embryogenesis we investigated the occurrence of autophagy in human embryonic stem cells. We have, using lentiviral transduction, established multiple human embryonic stem cell lines that stably express GFP-LC3, a fluorescent marker for the autophagosome. Each cell line displays both a normal karyotype and pluripotency as indicated by the presence of cell types representative of the three germlayers in derived teratomas. GFP expression and labelling of autophagosomes is retained after differentiation. Baseline levels of autophagy detected in cultured undifferentiated hESC were increased or decreased in the presence of rapamycin and wortmannin, respectively. Interestingly, autophagy was upregulated in hESCs induced to undergo differentiation by treatment with type I TGF-beta receptor inhibitor SB431542 or removal of MEF secreted maintenance factors. In conclusion we have established hESCs capable of reporting macroautophagy and identify a novel link between autophagy and early differentiation events in hESC.

  12. Sygeplejestuderendes brug af portfolio i klinisk undervisning

    DEFF Research Database (Denmark)

    Hjorth, Anne Charlotte Overgaard; Bruhn, Helle

    2014-01-01

    Brugen af portfolio var mangelfuld, men et udviklingsprojekt i samarbejde med den kommunale sygepleje motiverede både sygeplejestuderende og kliniske vejledere til at anvende læringsdelen af portfolio aktivt.......Brugen af portfolio var mangelfuld, men et udviklingsprojekt i samarbejde med den kommunale sygepleje motiverede både sygeplejestuderende og kliniske vejledere til at anvende læringsdelen af portfolio aktivt....

  13. Kortlægning af unge

    DEFF Research Database (Denmark)

    Skou, Carl Verner

    2009-01-01

    Computerprogram til Windows XP/Vista platformen til indberetning af unge under 18 år til registering af stofmisbrug og sociale data. Udgave: 1.0.1.1 Udgivelsesdato: 01-01-2009......Computerprogram til Windows XP/Vista platformen til indberetning af unge under 18 år til registering af stofmisbrug og sociale data. Udgave: 1.0.1.1 Udgivelsesdato: 01-01-2009...

  14. Udviklingen af rumopfattelsen 1600-2000

    DEFF Research Database (Denmark)

    Brock, Steen

    2010-01-01

    Hovedtræk af rumopfattelsens idehistorie fra Descartes til Wittgenstein med speciel vægt på Kant, værkbegrebet, modelbegrebet og den fænomenologiske opfattelse af rummet som handlingens sted......Hovedtræk af rumopfattelsens idehistorie fra Descartes til Wittgenstein med speciel vægt på Kant, værkbegrebet, modelbegrebet og den fænomenologiske opfattelse af rummet som handlingens sted...

  15. Human embryonic stem cells for neuronal repair.

    Science.gov (United States)

    Ben-Hur, Tamir

    2006-02-01

    Human embryonic stem cells may serve as a potentially endeless source of transplantable cells to treat various neurologic disorders. Accumulating data have shown the therapeutic value of various neural precursor cell types in experimental models of neurologic diseases. Tailoring cell therapy for specific disorders requires the generation of cells that are committed to specific neural lineages. To this end, protocols were recently developed for the derivation of dopaminergic neurons, spinal motor neurons and oligodendrocytes from hESC. These protocols recapitulate normal development in culture conditions. However, a novel concept emerging from these studies is that the beneficial effect of transplanted stem cells is not only via cell replacement in damaged host tissue, but also by trophic and protective effects, as well as by an immunomodulatory effect that down-regulates detrimental brain inflammation.

  16. Novel agents inhibit human leukemic cells

    Institute of Scientific and Technical Information of China (English)

    Wei-ping YU; Juan LI

    2012-01-01

    Ouabain (OUA) and pyrithione zinc (PZ) have been proved as the potential drugs for treating acute myeloid leukemia (AML).Selected from a screening among 1040 Food and Drug Administration-approved pharmacological agents,both drugs showability to induce apoptosis of the culturing AML cells,exhibiting the poisoning effect on the cells.Studies also reveal the efficiency of the drugs in inhibiting the growth of human AML cells injected into the mice lacking of immunity and killing primary AML cells from the peripheral blood of AML patients[1].

  17. Dimensionering af indblæsning

    DEFF Research Database (Denmark)

    Nielsen, Peter V.

    1974-01-01

    Dimensionering af indblæsningsarmaturer og bestemmelse af luftfordeling i lokaler bygger traditionelt på kendskabet til isotermiske fristråler og vægstråler. Som det fremgår af skitserne i indholdsfortegnelsen, indleder vi også med dette emne. Derefter udsætter vi - skridt for skridt - de ideele...

  18. Energioptimering af bæredygtighedscertificeringsprocesserne

    DEFF Research Database (Denmark)

    Christensen, Dorthe Holmberg; Carlsen, Mads; Alon, Shlomo Nevo

    2014-01-01

    Større fokus på bæredygtighedscertificeringer som DGNB har yderligere øget kompleksiteten af designet af bygninger. Introduktionen af teknologier som building information modelling (BIM) og proces metoder som integreret design proces (IDP) kan hjælpe med at reducere kompleksiteten og bedre...

  19. Forskningsbibliotekernes bidrag til produktion af evidensbaseret viden

    DEFF Research Database (Denmark)

    Johannsen, Carl Gustav

    2012-01-01

    Artiklen indeholder en karakteristik af evidensbevægelsens metodemæssige grundlag, hvor det pointeres, at evidensbevægelsen er et forgrenet, internationalt samrabejde om produktion og formidling af systematiske reviews vedr. effektiviteten af offentlige indsatser, interventioner, som hovedformål....

  20. Registrering af biometriske og biologiske personoplysninger

    DEFF Research Database (Denmark)

    Blume, Peter Erik; Herrmann, Janne Rothmar

    2009-01-01

    Med udgangspunkt i menneskeretsdomstolens Marper dom drøftes i artiklen indretningen af dansk rets regler om registrering af fingeraftryk og dna-profil med tilhørende biologisk materiale, og om dommen nødvendiggør ændringer af disse regler....

  1. Redskaber til monitorering af trafikken (REMOTE)

    DEFF Research Database (Denmark)

    Denne rapport samler op på erfaringerne fra REMOTE projektet. Rapporten r skrevet på en blanding af dansk og engelsk, da der både har været dansk og engelsk talende personer med i projektet. Den første del af rapporten beskriver overordnet, hvad der er sket i projektet. Herefter kommer der et afs...

  2. Regulering af jødiske kroppe

    DEFF Research Database (Denmark)

    Schleicher, Marianne

    2014-01-01

    på teori dels af Michel Foucault om, hvordan kultur sikrer sin egen overlevelse ved at indsamle viden om bl.a. kroppe med henblik på at regulere brugen af dem, dels af Judith Butler, der bl.a. forklarer, hvordan kultur trods alt er åben for såkaldt subversion, idet det bidrager til at udvide og...

  3. Måling af mineraluldsfibre i indeklimaet

    DEFF Research Database (Denmark)

    Nielsen, O.; Andersen, N.E.; Bredsdorff, P.;

    Rapporten beskriver resultaterne af målinger af luftbårne mineraluldsfibre, luftbårne ikke-mineraluldsfibre, luftbårent støv samt sedimenterede mineraluldsfibre i 105 lokaler med forskellige loftstyper. Resultaterne vil bl.a. kunne anvendes i forbindelse med vurdering af mulighederne for reduceri...

  4. Forskningsbibliotekernes bidrag til produktion af evidensbaseret viden

    DEFF Research Database (Denmark)

    Johannsen, Carl Gustav

    2012-01-01

    Artiklen indeholder en karakteristik af evidensbevægelsens metodemæssige grundlag, hvor det pointeres, at evidensbevægelsen er et forgrenet, internationalt samrabejde om produktion og formidling af systematiske reviews vedr. effektiviteten af offentlige indsatser, interventioner, som hovedformål....

  5. Registrering og Benchmarking af Modus 2 Forskning

    DEFF Research Database (Denmark)

    Duus, Henrik Johannsen; Jørgensen, Jens E.

    2004-01-01

    Fremvæksten af modus 2 forskning stiller krav om en revurdering af højere læreanstalters evaluerings- og meriteringspraksis. Den endimensionale prioritering af modus 1 forskning såvel som dennes ligeså endimensionale evaluering via publikationer må afvises som utidssvarende. Som en konsekvens her...

  6. Modelanalyser af mobilitet og miljø

    DEFF Research Database (Denmark)

    Christensen, Linda; Gudmundsson, Henrik

    Begrebet bæredygtig transport diskuteres og ved hjælp af persontrafikmodellen ALTRANS belyses mulighederne for at fremme bæredygtig udvikling gennem en række scenarier. Desuden præsenteres en mere detaljeret analyse af ændringer i CO2-belastningen ved forbedring af den kollektive trafiks servicen...

  7. Identifikation af referencevandløb til implementering af vandrammedirektivet i Danmark

    DEFF Research Database (Denmark)

    Kristensen, Esben Astrup; Baattrup-Pedersen, Annette; Skriver, Jens

    Definition og beskrivelse af referencetilstanden er centralt for implementering af vandrammedirektivet, da bedømmelse af den økologiske tilstand sker som afvigelsen fra den upåvirkede referencetilstand. I denne rapport præsenteres analyser af danske, udenlandske og historiske data med det formål...

  8. Anmeldelse af Attila Aspelunds oversættelse af Ducharmes 'Slugerens slugt.'

    DEFF Research Database (Denmark)

    Hansen, Troels Hughes

    2016-01-01

    En oversættelsesfaglig gennemgang af oversættelsen fra Québec-fransk til dansk af Réjean Ducharmes berømte bog.......En oversættelsesfaglig gennemgang af oversættelsen fra Québec-fransk til dansk af Réjean Ducharmes berømte bog....

  9. Fermentering af korn øger fordøjeligheden af næringsstoffer

    DEFF Research Database (Denmark)

    Pedersen, Anni Øyan; Jørgensen, Henry Johs. Høgh; Knudsen, Knud Erik Bach;

    2010-01-01

    Et fordøjelighedsforsøg har vist, at energiværdien af byg og hvede steg ved fermentering. Effekten var størst ved fermentering af byg, hvor fordøjeligheden af organisk stof for enden af tyndtarmen steg statistisk sikkert med 9 %. Ved fermentering af hvede steg fordøjeligheden af organisk stof...... statistisk sikkert med 3 %. Stigningen i energiværdien ved fermentering af korn kunne ikke vises ved foderanalyser (FEsv), hvilket viser, at in vitro-analyserne EFOS og EFOSi ikke kan anvendes til vurdering af effekten af fermentering. Fordøjeligheden af fosfor i foderblandinger baseret på byg eller hvede og...... tilskudsfoder steg statistisk sikkert ved fermentering af kornet. Der blev ikke tilsat mikrobiel fytase. Ved fermentering af byg steg fordøjeligheden af fosfor i foderblandingen fra 32 til 47 %, og ved fermentering af hvede steg fordøjeligheden af fosfor i blandingen fra 43 til 57 %. Fordøjeligheden af fosfor i...

  10. Isolation and antiproliferative activity of Lotus corniculatus lectin towards human tumour cell lines.

    Science.gov (United States)

    Rafiq, Shaista; Majeed, Rabiya; Qazi, Asif Khurshid; Ganai, Bashir Ahmad; Wani, Ishfak; Rakhshanda, Syed; Qurishi, Yasrib; Sharma, P R; Hamid, Abid; Masood, Akbar; Hamid, Rabia

    2013-12-15

    The objective of the study was to investigate the anti cancer activity of a lectin isolated from Lotus corniculatus seeds. A tetrameric 70kDa galactose specific lectin was purified using two step simple purification protocol which involved affinity chromatography on AF-BlueHC650M and gel filtration on Sephadex G-100. The lectin was adsorbed on AF-BlueHC650M and desorbed using 1M NaCl in the starting buffer. Gel filtration on Sephadex G-100 yielded a major peak absorbance that gave two bands of 15kDa and 20kDa in SDS PAGE. Hemagglutination activity was completely preserved, when the temperature was in the range of 20-60°C. However, drastic reduction in activity occurred at temperatures above 60°C. Full hemagglutination activity was retained at ambient pH 4-12. Thereafter no activity was observed above pH 13. Hemaglutination of the lectin was inhibited by d-galactose. The lectin showed a strong antiproliferative activity towards human leukemic (THP-1) cancer cells followed by lung cancer (HOP62) cells and HCT116 with an IC50 of 39μg/ml and 50μg/ml and 60μg/ml respectively. Flow cytometry analysis showed an increase in the percentage of cells in sub G0G1 phase confirming that Lotus corniculatus lectin induced apoptosis. Morphological observations showed that Lotus corniculatus lectin (LCL) treated THP-1 cells displayed apparent apoptosis characteristics such as nuclear fragmentation, appearance of membrane enclosed apoptotic bodies and DNA fragmentation. Lotus corniculatus lectin (LCL) effectively inhibits the cell migration in a dose dependent manner as indicated by the wound healing assay.

  11. 3 CFR - Guidelines for Human Stem Cell Research

    Science.gov (United States)

    2010-01-01

    ... 3 The President 1 2010-01-01 2010-01-01 false Guidelines for Human Stem Cell Research Presidential Documents Other Presidential Documents Memorandum of July 30, 2009 Guidelines for Human Stem Cell Research..., scientifically worthy human stem cell research, including human embryonic stem cell research, to the extent...

  12. Produktion og afsætning af nicheprodukter

    DEFF Research Database (Denmark)

    Jensen, Jonas Baungaard; Hansen, Henning Otte

    2016-01-01

    . Egnsmærker opnår generelt en merpris på 20-30 pct. Det er dog bemærkelsesværdigt, at landmanden “kun” har omkring 13 pct. af forbrugerkronen på Lammefjords-kartofler. Her er der tale om unikke produkter, hvor det i praksis udelukkende er landmanden og landmandens placering og produktion, som skaber de unikke...

  13. Trafikanalyser af et net af letbaner og BRT i Hovedstaden

    DEFF Research Database (Denmark)

    Nielsen, Otto Anker; Ingvardson, Jesper Bláfoss; Andersen, Jonas Lohmann Elkjær

    Denne rapport gennemgår et projekt for Region Hovedstaden, hvor letbanenet fra forårets opgave for Region Hovedstaden (Nielsen, Ingvardson & Andersen, 2013) blev detaljeret, viderebearbejdet og forbedret, hvorefter der er gennemført beregninger af forskellige varianter med OTM Trafikmodellen. Arb...... og sammenlignes med tidligere net og beregninger, der er blevet gennemført i Trængselskommissionens regi....

  14. Undersøgelse af kommunernes administration af bygningsreglementets elevatorkrav

    DEFF Research Database (Denmark)

    Grangaard, Sidse

    få dispensationsansøgninger for elevator ved nybyggeri. Undersøgelsen tyder dog på, at nogle bygherrer og rådgivere er opmærksomme på kravet og i nogle tilfælde vælger at disponere bygningen, så der ikke stilles krav om elevator. Ved ombygning og tilbygning oplever langt over halvdelen af kommunerne...

  15. Myristoylation profiling in human cells and zebrafish

    Directory of Open Access Journals (Sweden)

    Malgorzata Broncel

    2015-09-01

    Full Text Available Human cells (HEK 293, HeLa, MCF-7 and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC. This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223–6 (PXD001863 and PXD001876 and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.

  16. Human cells derived from degenerate intervertebral discs respond differently to those derived from non-degenerate intervertebral discs following application of dynamic hydrostatic pressure.

    Science.gov (United States)

    Le Maitre, Christine Lyn; Frain, Jennie; Fotheringham, Andrew P; Freemont, Anthony J; Hoyland, Judith Alison

    2008-01-01

    The intervertebral disc (IVD) is one of the body's most important load-bearing structures with the major mechanical force experienced in the nucleus pulposus (NP) being hydrostatic pressure (HP). Physiological levels of HP have an anabolic effect on IVD matrix metabolism in cells derived from non-degenerate animal and herniated IVD while excessive HP has a catabolic effect. However, no studies have investigated the response of non-degenerate and degenerate human disc cells derived from non-herniated discs to HP. Here we investigate the effect of physiological HP on such cells using a novel loading rig. Human IVD cells (both NP and AF) cultured in alginate were subjected to dynamic HP (0.8-1.7 MPa 0.5 Hz) for 2 h. Cell viability was assessed, RNA extracted and qRT-PCR for 18 s, c-fos, Sox-9, collagen type II, aggrecan and MMP-3 performed. Cell viability was unaffected by the loading regime. In non-degenerate NP cells, HP increased c-fos, aggrecan, Sox-9 and collagen type II (significantly so in the case of c-fos and aggrecan), but not MMP-3 gene expression. In contrast, application of HP to AF or degenerate NP cells had no effect on target gene expression. Our data shows that cells obtained from the healthy NP respond to dynamic HP by up-regulating genes indicative of healthy matrix homeostasis. However, responses differed in degenerate NP cells suggesting that an altered mechanotransduction pathway may be operational.

  17. Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

    Science.gov (United States)

    Fukusumi, Hayato; Shofuda, Tomoko; Bamba, Yohei; Yamamoto, Atsuyo; Kanematsu, Daisuke; Handa, Yukako; Okita, Keisuke; Nakamura, Masaya; Yamanaka, Shinya; Okano, Hideyuki; Kanemura, Yonehiro

    2016-01-01

    Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes. PMID:27212953

  18. Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins.

    Science.gov (United States)

    Fukusumi, Hayato; Shofuda, Tomoko; Bamba, Yohei; Yamamoto, Atsuyo; Kanematsu, Daisuke; Handa, Yukako; Okita, Keisuke; Nakamura, Masaya; Yamanaka, Shinya; Okano, Hideyuki; Kanemura, Yonehiro

    2016-01-01

    Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

  19. Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell lines.

    Science.gov (United States)

    Zou, Chunlin; Chou, Bin-Kuan; Dowey, Sarah N; Tsang, Kitman; Huang, Xiaosong; Liu, Cyndi F; Smith, Cory; Yen, Jonathan; Mali, Prashant; Zhang, Yu Alex; Cheng, Linzhao; Ye, Zhaohui

    2012-08-10

    Derivation of pluripotent stem cells (iPSCs) induced from somatic cell types and the subsequent genetic modifications of disease-specific or patient-specific iPSCs are crucial steps in their applications for disease modeling as well as future cell and gene therapies. Conventional procedures of these processes require co-culture with primary mouse embryonic fibroblasts (MEFs) to support self-renewal and clonal growth of human iPSCs as well as embryonic stem cells (ESCs). However, the variability of MEF quality affects the efficiencies of all these steps. Furthermore, animal sourced feeders may hinder the clinical applications of human stem cells. In order to overcome these hurdles, we established immortalized human feeder cell lines by stably expressing human telomerase reverse transcriptase, Wnt3a, and drug resistance genes in adult mesenchymal stem cells. Here, we show that these immortalized human feeders support efficient derivation of virus-free, integration-free human iPSCs and long-term expansion of human iPSCs and ESCs. Moreover, the drug-resistance feature of these feeders also supports nonviral gene transfer and expression at a high efficiency, mediated by piggyBac DNA transposition. Importantly, these human feeders exhibit superior ability over MEFs in supporting homologous recombination-mediated gene targeting in human iPSCs, allowing us to efficiently target a transgene into the AAVS1 safe harbor locus in recently derived integration-free iPSCs. Our results have great implications in disease modeling and translational applications of human iPSCs, as these engineered human cell lines provide a more efficient tool for genetic modifications and a safer alternative for supporting self-renewal of human iPSCs and ESCs.

  20. Argyrophilic nucleolar organizer regions (AgNORs) in odontogenic myxoma (OM) and ameloblastic fibroma (AF).

    Science.gov (United States)

    Martins, C; Carvalho, Y R; do Carmo, M A

    2001-09-01

    Ten cases of odontogenic myxoma (OM) and six cases of ameloblastic fibroma (AF) were subjected to comparative analysis by the AgNOR technique, in order to determine a possible difference in cell proliferation index between these lesions. The mean AgNOR number of the mesenchymal component of AF was compared with its epithelial component and the difference was not found to be statistically significant. The mean AgNOR index of the AF group was significantly higher than that of the OM group. Moreover, the mesenchymal component of AF demonstrated increased AgNOR numbers compared with that of OM (P<0.05). These results suggest that the epithelial and mesenchymal components of AF may have similar cell proliferative activity. However, the cell proliferative index of this lesion seems to be higher than that of OM.

  1. Overvågning af influenza i svin 2012

    DEFF Research Database (Denmark)

    Breum, Solvej Østergaard; Hjulsager, Charlotte Kristiane; Larsen, Lars Erik

    måneder, men influenza virus påvises med samme prævalens hele året. I alt 78 influenzavirus positive indsendelser blev undersøgt ved sekvensanalyse for at bestemme subtypen. Resultatet af disse analyser viste, at de to mest almindelige subtyper i danske svin er den danske variant af H1N2 og H1N1 (avian...... aspekter ved influenza A virus i svin i Danmark. Undersøgelserne har med stor sandsynlighed vist at H1N1pdm09, som stadig må betragtes som en zoonose, nu er etableret i den danske svine population, hvor den cirkulerer uafhængigt af den humane influenzavirus sæson. Overvågningen har endvidere vist...... influenzavirus positive prøver for pandemisk H1N1 (H1N1pdm09) ved real time RT-PCR der specifikt detekterer H-genet i H1N1pdm09 virus; 3) Subtypning af positive prøver ved sekvensanalyse (H og N generne); 4) Komplet genom karakterisering af udvalgte virusisolater. Der er totalt set iværksat undersøgelse...

  2. [Human pluripotent stem cell and neural differentiation].

    Science.gov (United States)

    Wataya, Takafumi; Muguruma, Keiko; Sasai, Yoshiki

    2008-10-01

    Recovery of lost brain function is an important issue in medical studies because neurons of the central nervous system (CNS) have poor potential for regeneration. Since few CNS diseases can be treated completely by medicines, regenerative therapy by using stem cells should be studied as a new type of therapeutic intervention. The efficacy of cell replacement therapy in Parkinson's disease has been well investigated. Several studies on fetal tissue transplantation have revealed that quantity and purity of transplanted cells are necessary for recovery of symptoms. SFEB (Serum-free floating culture of embryoid body-like aggregates) method is capable of inducing multi-potential CNS progenitors that can be steered to differentiate into region-specific tissues. On the basis of the existing knowledge of embryology, we have succeeded in the generating of various types of neurons such as telencephalic, cerebeller (Purkinje and granule cells), retinal (photoreceptor cells) and hypothalamic neurons. Application of this culture method to human ES (hES) cells is necessary for clinical purpose: however, poor survival of hES cells in SFEB culture might limit the possibility of using these cells for future medical applications. We found that a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, markedly diminished the dissociation-induced apoptosis of hES cells and enabled the cells to form aggregates in SFEB culture. For both mouse and human ES cells, SFEB culture is a favorable method that can generate large amounts of region-specific neurons. However, stem cell-based therapy continues to face several obstacles. It is important that researchers in the basic sciences and clinical medicine should discuss these problems together to overcome both scientific and ethical issues related to stem cells.

  3. T-cell response in human leishmaniasis

    DEFF Research Database (Denmark)

    Kharazmi, A; Kemp, K; Ismail, A

    1999-01-01

    In the present communication we provide evidence for the existence of a Th1/Th2 dichotomy in the T-cell response to Leishmania antigens in human leishmaniasis. Our data suggest that the pattern of IL-4 and IFN-gamma response is polarised in these patients. Lymphocytes from individuals recovered......+. Furthermore, IL-10 plays an important role in the development of post kala azar dermal leishmaniasis (PKDL) from VL. The balance between the parasitic-specific T-cell response plays an important regulatory role in determining the outcome of Leishmania infections in humans....

  4. Characterization of human pluripotent stem cells.

    Science.gov (United States)

    Gokhale, Paul J; Andrews, Peter W

    2013-12-18

    Human pluripotent stem cells (PSCs), whether embryonic stem cells or induced PSCs, offer enormous opportunities for regenerative medicine and other biomedical applications once we have developed the ability to harness their capacity for extensive differentiation. Central to this is our ability to identify and characterize such PSCs, but this is fraught with potential difficulties that arise from a tension between functional definitions of pluripotency and the more convenient use of 'markers', a problem exacerbated by ethical issues, our lack of knowledge of early human embryonic development, and differences from the mouse paradigm.

  5. CLOSTRIDIUM SPORE ATTACHMENT TO HUMAN CELLS

    Energy Technology Data Exchange (ETDEWEB)

    PANESSA-WARREN,B.; TORTORA,G.; WARREN,J.

    1997-08-10

    This paper uses high resolution scanning electron microscopy (SEM) with a LaB6 gun and the newest commercial field emission guns, to obtain high magnification images of intact clostridial spores throughout the activation/germination/outgrowth process. By high resolution SEM, the clostridial exosporial membrane can be seen to produce numerous delicate projections (following activation), that extend from the exosporial surface to a nutritive substrate (agar), or cell surface when anaerobically incubated in the presence of human cells (embryonic fibroblasts and colon carcinoma cells). Magnifications of 20,000 to 200,000Xs at accelerating voltages low enough to minimize or eliminate specimen damage (1--5 kV) have permitted the entire surface of C.sporogenes and C.difficile endospores to be examined during all stages of germination. The relationships between the spore and the agar or human cell surface were also clearly visible.

  6. Human pluripotent stem cells in contemporary medicine

    Directory of Open Access Journals (Sweden)

    S. A. Rodin

    2015-01-01

    Full Text Available Human pluripotent stem cells (hPSCs are capable of indefinite proliferation and can be differentiated into any cell type of the human body. Therefore, they are a promising source of cells for treatment of numerous degenerative diseases and injuries. Pluripotent stem cells are also associated with a number of ethical, safety and technological issues. In this review, we describe various types of hPSCs, safety issues that concern all or some types of hPSCs and methods of clinical-grade hPSC line development. Also, we discuss current and past clinical trials involving hPSCs, their outcomes and future perspectives of hPSC-based therapy. 

  7. Cell pattern in adult human corneal endothelium.

    Directory of Open Access Journals (Sweden)

    Carlos H Wörner

    Full Text Available A review of the current data on the cell density of normal adult human endothelial cells was carried out in order to establish some common parameters appearing in the different considered populations. From the analysis of cell growth patterns, it is inferred that the cell aging rate is similar for each of the different considered populations. Also, the morphology, the cell distribution and the tendency to hexagonallity are studied. The results are consistent with the hypothesis that this phenomenon is analogous with cell behavior in other structures such as dry foams and grains in polycrystalline materials. Therefore, its driving force may be controlled by the surface tension and the mobility of the boundaries.

  8. Merkel cell distribution in the human eyelid

    Directory of Open Access Journals (Sweden)

    C.A. May

    2013-10-01

    Full Text Available Although Merkel cell carcinoma of the eye lid is reported frequently in the literature, only limited information exists about the distribution of Merkel cells in this tissue. Therefore, serial sections of 18 human cadaver eye lids (donors ages ranging between 63 and 97 years were stained for cytokeratin 20 in various planes. The overall appearance of Merkel cells in these samples was low and mainly located in the outer root layer of the cilia hair follicles. Merkel cells were more frequent in the middle, and almost not detectable at the nasal and temporal edges. The localization is in accordance with that of Merkel cell carcinoma, but concerning the scarce appearance within this adulthood group, a specific physiological role of these cells in the eye lid is difficult to establish.

  9. Natural killer cells in human autoimmune disorders

    Science.gov (United States)

    2013-01-01

    Natural killer (NK) cells are innate lymphocytes that play a critical role in early host defense against viruses. Through their cytolytic capacity and generation of cytokines and chemokines, NK cells modulate the activity of other components of the innate and adaptive immune systems and have been implicated in the initiation or maintenance of autoimmune responses. This review focuses on recent research elucidating a potential immunoregulatory role for NK cells in T-cell and B-cell-mediated autoimmune disorders in humans, with a particular focus on multiple sclerosis, rheumatoid arthritis, and systemic lupus erythematous. A better understanding of the contributions of NK cells to the development of autoimmunity may lead to novel therapeutic targets in these diseases. PMID:23856014

  10. Måling af diskrimination på baggrund af etnisk oprindelse

    DEFF Research Database (Denmark)

    Jensen, Tina Gudrun; Weibel, Kristina; Tørslev, Mette Kirstine

    Denne rapport sætter fokus på, hvordan man kan måle diskrimination på baggrund af etnisk oprindelse. På baggrund af et studie af tidligere undersøgelser på området og relevant metodelitteratur, søger rapporten at udvikle metoder til valide målinger af diskrimination på baggrund af etnisk oprindel...

  11. Human Colon Cancer Cells Cultivated in Space

    Science.gov (United States)

    1995-01-01

    Within five days, bioreactor cultivated human colon cancer cells (shown) grown in Microgravity on the STS-70 mission in 1995, had grown 30 times the volume of the control specimens on Earth. The samples grown in space had a higher level of cellular organization and specialization. Because they more closely resemble tumors found in the body, microgravity grown cell cultures are ideal for research purposes.

  12. Quercetin Inhibits Cell Migration and Invasion in Human Osteosarcoma Cells

    Directory of Open Access Journals (Sweden)

    Haifeng Lan

    2017-09-01

    Full Text Available Background/Aims: Osteosarcoma is a malignant tumor associated with high mortality; however, no effective therapies for the disease have been developed. Several studies have focused on elucidating the pathogenesis of osteosarcoma and have aimed to develop novel therapies for the disease. Quercetin is a vital dietary flavonoid that has been shown to have a variety of anticancer effects, as it induces cell cycle arrest, apoptosis, and differentiation and is involved in cell adhesion, metastasis and angiogenesis. Herein, we aimed to investigate the effects of quercetin on osteosarcoma migration and invasion in vitro and in vivo and to explore the molecular mechanisms underlying its effects on osteosarcoma migration and invasion. Methods: Cell viability, cell cycle activity and cell apoptosis were measured using CCK-8 assay and flow cytometry, and cell migration and invasion were evaluated by wound healing and transwell assays, respectively. The mRNA and protein expression levels of several proteins of interest were assessed by real-time quantitative PCR and western blotting, respectively. Moreover, a nude mouse model of human osteosarcoma lung metastasis was established to assess the anti-metastatic effects of quercetin in vivo. Results: We noted no significant differences in cell cycle activity and apoptosis between HOS and MG63 cells and control cells. Treatment with quercetin significantly attenuated cell migration and invasion in HOS and MG63 cells compared with treatment with control medium. Moreover HIF-1α, VEGF, MMP2, and MMP9 mRNA and protein expression levels were significantly downregulated in HOS cells treated with quercetin compared with HOS cells treated with controls. Additionally, treatment with quercetin attenuated metastatic lung tumor formation and growth in the nude mouse model of osteosarcoma compared with treatment with controls. Conclusion: Our findings regarding the inhibitory effects of quercetin on cell migration and

  13. Human Mammary Luminal Epithelial Cells Contain Progenitors to Myoepithelial Cells

    Energy Technology Data Exchange (ETDEWEB)

    Pechoux, Christine; Gudjonsson, Thorarinn; Ronnov-Jessen, Lone; Bissell, Mina J; Petersen, Ole

    1999-02-01

    The origin of the epithelial and myoepithelial cells in the human breast has not been delineated. In this study we have addressed whether luminal epithelial cells and myoepithelial cells are vertically connected, i.e., whether one is the precursor for the other. We used a primary culture assay allowing preservation of basic phenotypic traits of luminal epithelial and myoepithelial cells in culture. The two cell types were then separated immunomagnetically using antibodies directed against lineage-specific cell surface antigens into at best 100% purity. The cellular identity was ascertained by cytochemistry, immunoblotting, and 2-D gel electrophoresis. Luminal epithelial cells were identified by strong expression of cytokeratins 18 and 19 while myoepithelial cells were recognized by expression of vimentin and {alpha}-smooth muscle actin. We used a previously devised culture medium (CDM4) that allows vigorous expansion of proliferative myoepithelial cells and also devised a medium (CDM6) that allowed sufficient expansion of differentiated luminal epithelial cells based on addition of hepatocyte growth factor/scatter factor. The two different culture media supported each lineage for at least five passages without signs of interconversion. We used parallel cultures where we switched culture media, thus testing the ability of each lineage to convert to the other. Whereas the myoepithelial lineage showed no signs of interconversion, a subset of luminal epithelial cells, gradually, but distinctly, converted to myoepithelial cells. We propose that in the mature human breast, it is the luminal epithelial cell compartment that gives rise to myoepithelial cells rather than the other way around.

  14. Genetic Manipulation of Human Embryonic Stem Cells.

    Science.gov (United States)

    Eiges, Rachel

    2016-01-01

    One of the great advantages of embryonic stem (ES) cells over other cell types is their accessibility to genetic manipulation. They can easily undergo genetic modifications while remaining pluripotent, and can be selectively propagated, allowing the clonal expansion of genetically altered cells in culture. Since the first isolation of ES cells in mice, many effective techniques have been developed for gene delivery and manipulation of ES cells. These include transfection, electroporation, and infection protocols, as well as different approaches for inserting, deleting, or changing the expression of genes. These methods proved to be extremely useful in mouse ES cells, for monitoring and directing differentiation, discovering unknown genes, and studying their function, and are now being extensively implemented in human ES cells (HESCs). This chapter describes the different approaches and methodologies that have been applied for the genetic manipulation of HESCs and their applications. Detailed protocols for generating clones of genetically modified HESCs by transfection, electroporation, and infection will be described, with special emphasis on the important technical details that are required for this purpose. All protocols are equally effective in human-induced pluripotent stem (iPS) cells.

  15. Forskningsoversigt - Effekterne af Cooperative Learning

    DEFF Research Database (Denmark)

    Larsen, Lea Lund

    Kan Cooperative Learning - en undervisningsform hvor lærerens tid ved tavlen mindskes og hvor de lærende samarbejder om stoffet - maksimere de lærendes indlæring og medvirke til en forbedring af deres interpersonelle og kommunikative kompetencer, samt øge deres motivation for læring? Den megen...... Learning, som didaktisk metode være medvirkende til at imødekomme de komplekse krav, der stilles for at udvikle de lærendes faglige, sociale og kommunikative sider på tværs af forskellighederne. Når denne viden bringes over i en voksenuddannelsessammenhæng er det interessant at se på, om der er evidens for...

  16. Halvering af dagpengeperioden og akutpakken

    DEFF Research Database (Denmark)

    Nilsson, Klara; Holt, Helle

    Formålet med denne undersøgelse er at give et øjebliksbillede af jobcentrenes og a-kassernes beskæftigelsesindsats her og nu. Gennem en række kvalitative interview med medarbejdere fra jobcentre og a-kasser belyses det, hvordan henholdsvis jobcentre og a-kasser har håndteret halveringen af...... at finde et job, hvilket blandt andet har betydet en tidligere og intensiveret indsats overfor de ledige. Den særlige indsats har derfor bidraget med et større fokus og sammenhæng i indsatsen for den enkelte borger gennem blandt andet en personlig jobformidler og fællessamtaler mellem den ledige, a...

  17. Vaccination af slagtekalve mod BRSV

    DEFF Research Database (Denmark)

    Graumann, Anne Mette; Larsen, Lars Erik

    2009-01-01

    Rspiratory Suncytial Virus) blev fundet til at være den typisk fundne virus, der forårsager lungebetændelse. Vacciner imod BRSV er tilgængelige på markedet, men de er aldrig blevt afprøvet systematisk under danske forhold. Formålet med dette forsøg er at undersøge effekten af to forskellige...

  18. Konstruktionen af det selvstyrende jobcenter

    DEFF Research Database (Denmark)

    Hjortsø Pedersen, Maria; Hansen, Agnete Meldgaard

    2011-01-01

    Forvaltningsstrukturen på beskæftigelsesområdet har over de seneste år gennemgået en række ændringer, hvor ansvaret for beskæftigelsesindsatsen bl.a. er blevet decentraliseret til kommunerne. Denne decentralisering er dog gået hånd i hånd med statslige ambitioner om stærk styring af området. For ...

  19. Ledelsesroller i globalisering af cleantech

    DEFF Research Database (Denmark)

    Knudsen, Poul Frøjk; Tambo, Torben

    2011-01-01

    Cleantech anses i de fleste industrialiserede lande for den nye vækstbølge, der både skal fastholde arbejdspladser og beskæftigelse og også redde miljøet. Danmark har været early-mover på vindkraft. Aktiv ledelse har til dato fastholdt et forspring. Denne aktive ledelse har sikret anvendelse af...

  20. Enriched retinal ganglion cells derived from human embryonic stem cells

    Science.gov (United States)

    Gill, Katherine P.; Hung, Sandy S. C.; Sharov, Alexei; Lo, Camden Y.; Needham, Karina; Lidgerwood, Grace E.; Jackson, Stacey; Crombie, Duncan E.; Nayagam, Bryony A.; Cook, Anthony L.; Hewitt, Alex W.; Pébay, Alice; Wong, Raymond C. B.

    2016-01-01

    Optic neuropathies are characterised by a loss of retinal ganglion cells (RGCs) that lead to vision impairment. Development of cell therapy requires a better understanding of the signals that direct stem cells into RGCs. Human embryonic stem cells (hESCs) represent an unlimited cellular source for generation of human RGCs in vitro. In this study, we present a 45-day protocol that utilises magnetic activated cell sorting to generate enriched population of RGCs via stepwise retinal differentiation using hESCs. We performed an extensive characterization of these stem cell-derived RGCs by examining the gene and protein expressions of a panel of neural/RGC markers. Furthermore, whole transcriptome analysis demonstrated similarity of the hESC-derived RGCs to human adult RGCs. The enriched hESC-RGCs possess long axons, functional electrophysiological profiles and axonal transport of mitochondria, suggestive of maturity. In summary, this RGC differentiation protocol can generate an enriched population of functional RGCs from hESCs, allowing future studies on disease modeling of optic neuropathies and development of cell therapies. PMID:27506453

  1. Human ES cells: starting culture from frozen cells.

    Science.gov (United States)

    Trish, Erin; Dimos, John; Eggan, Kevin

    2006-11-09

    Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock. First, a one to two day old ten cm plate of approximately one (to two) million irradiated mouse embryonic fibroblast feeder cells is rinsed with HuES media to remove residual serum and cell debris, and then HuES media added and left to equilibrate in the cell culture incubator. A frozen vial of cells from long term liquid nitrogen storage or a -80 C freezer is sourced and quickly submerged in a 37 C water bath for quick thawing. Cells in freezing media are then removed from the vial and placed in a large volume of HuES media. The large volume of HuES media facilitates removal of excess serum and DMSO, which can cause HuES human embryonic stem cells to differentiate. Cells are gently spun out of suspension, and then re-suspended in a small volume of fresh HuES media that is then used to seed the MEF plate. It is considered important to seed the MEF plate by gently adding the HuES cells in a drop wise fashion to evenly disperse them throughout the plate. The newly established HuES culture plate is returned to the incubator for 48 hrs before media is replaced, then is fed every 24 hours thereafter.

  2. Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells

    Directory of Open Access Journals (Sweden)

    Hong-Mei An

    2013-12-01

    Full Text Available Hepatocarcinogenesis is associated with epigenetic changes, including histone deacetylases (HDACs. Epigenetic modulation by HDAC inhibition is a potentially valuable approach for hepatocellular carcinoma treatment. In present study, we evaluated the anticancer effects of sodium valproate (SVP, a known HDAC inhibitor, in human hepatocarcinoma cells. The results showed SVP inhibited the proliferation of Bel-7402 cells in a dose-dependent manner. Low dose SVP treatment caused a large and flat morphology change, positive SA-β-gal staining, and G0/G1 phase cell cycle arrest in human hepatocarcinoma cells. Low dose SVP treatment also increased acetylation of histone H3 and H4 on p21 promoter, accompanied by up-regulation of p21 and down-regulation of RB phosphorylation. These observations suggested that a low dose of SVP could induce cell senescence in hepatocarcinoma cells, which might correlate with hyperacetylation of histone H3 and H4, up-regulation of p21, and inhibition of RB phosphorylation. Since the effective concentration inducing cell senescence in hepatocarcinoma cells is clinically available, whether a clinical dose of SVP could induce cell senescence in clinical hepatocarcinoma is worthy of further study.

  3. Human embryonic stem cell lines model experimental human cytomegalovirus latency.

    Science.gov (United States)

    Penkert, Rhiannon R; Kalejta, Robert F

    2013-05-28

    Herpesviruses are highly successful pathogens that persist for the lifetime of their hosts primarily because of their ability to establish and maintain latent infections from which the virus is capable of productively reactivating. Human cytomegalovirus (HCMV), a betaherpesvirus, establishes latency in CD34(+) hematopoietic progenitor cells during natural infections in the body. Experimental infection of CD34(+) cells ex vivo has demonstrated that expression of the viral gene products that drive productive infection is silenced by an intrinsic immune defense mediated by Daxx and histone deacetylases through heterochromatinization of the viral genome during the establishment of latency. Additional mechanistic details about the establishment, let alone maintenance and reactivation, of HCMV latency remain scarce. This is partly due to the technical challenges of CD34(+) cell culture, most notably, the difficulty in preventing spontaneous differentiation that drives reactivation and renders them permissive for productive infection. Here we demonstrate that HCMV can establish, maintain, and reactivate in vitro from experimental latency in cultures of human embryonic stem cells (ESCs), for which spurious differentiation can be prevented or controlled. Furthermore, we show that known molecular aspects of HCMV latency are faithfully recapitulated in these cells. In total, we present ESCs as a novel, tractable model for studies of HCMV latency.

  4. Brug af alternativ isolering i Finland og Sverige

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place

    2001-01-01

    Resume af rapport om anvendelse af alternative isoleringsmaterialer i Finland og Sverige, udarbejdet af SBI under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering".......Resume af rapport om anvendelse af alternative isoleringsmaterialer i Finland og Sverige, udarbejdet af SBI under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"....

  5. The M sub 1 muscarinic receptor and its second messenger coupling in human neuroblastoma cells and transfected murine fibroblast cells

    Energy Technology Data Exchange (ETDEWEB)

    Mei, Lin.

    1989-01-01

    The data of this study indicate that pirenzepine (PZ)-high affinity muscarinic receptors (mAChRs) are coupled to the hydrolysis of inositol lipids and not to the adenylate cyclase system in human neuroblastoma SH-SY5Y cells. The maximal carbachol(CCh)-stimulated ({sup 3}H)IP{sub 1} accumulation in the SH-SY5Y cells was decreased in the presence of 1{mu}g/ml pertussis toxin, suggesting that a pertussis toxin sensitive G-protein may be involved in the coupling. Several cell clones which express only M{sub 1} mAChR were generated by transfecting the murine fibroblast B82 cells with the cloned rat genomic m{sub 1} gene. The transfected B82 cells (cTB10) showed specific ({sup 3}H)(-)QNB binding activity. The mAChRs in these cells are of the M{sub 1} type defined by their high affinity for PZ and low affinity for AF-DX 116 and coupled to hydrolysis of inositol lipids, possibly via a pertussis toxin sensitive G protein. The relationship between the M{sub 1} mAChR density and the receptor-mediated hydrolysis of inositol lipids was studied in 7 clones. The M{sub 1} mAChR densities in these cells characterized by ({sup 3}H)(-)MQNB binding ranged from 12 fmol/10{sup 6} cells in LK3-1 cells to 260 fmol/10{sup 6} cells in the LK3-8 cells.

  6. [Immune system evolution. (From cells to humans)].

    Science.gov (United States)

    Belek, A S

    1992-01-01

    The great variety of cells and molecules observed in the mammalian immune system can be explained by stepwise acquisition of them during phylogeny. Self/nonself discrimination and cell-mediated immunity have been present since the early stages of evolution. Although some inducible antimicrobial molecules have been demonstrated in invertebrates, immunoglobulins appear in vertebrates. T and B cell diversity, development of the lymphoid organs, MHC molecules, complement and cytokines are the characteristics that appear through the evolution of vertebrates. Further knowledge that will be obtained from phylogenetic studies will improve our understanding of the immune system of human.

  7. Centre for human development, stem cells & regeneration.

    Science.gov (United States)

    Oreffo, Richard O C

    2014-01-01

    The Centre for Human Development, Stem Cells and Regeneration (CHDSCR) was founded in 2004 as a cross-disciplinary research and translational program within the Faculty of Medicine at the University of Southampton. The Centre undertakes fundamental research into early development and stem cells together with applied translational research for patient benefit. The Centre has vibrant and thriving multidisciplinary research programs that harness the translational strength of the Faculty together with an innovative Stem Cell PhD program, outstanding clinical infrastructure and enterprise to deliver on this vision.

  8. Biobanking human embryonic stem cell lines

    DEFF Research Database (Denmark)

    Holm, Søren

    2016-01-01

    Stem cell banks curating and distributing human embryonic stem cells have been established in a number of countries and by a number of private institutions. This paper identifies and critically discusses a number of arguments that are used to justify the importance of such banks in policy...... are curiously absent from the particular stem cell banking policy discourse. This to some extent artificially isolates this discourse from the broader discussions about the flows of reproductive materials and tissues in modern society, and such isolation may lead to the interests of important actors being...

  9. Advances in human B cell phenotypic profiling

    Directory of Open Access Journals (Sweden)

    Denise A Kaminski

    2012-10-01

    Full Text Available To advance our understanding and treatment of disease, research immunologists have been called-upon to place more centralized emphasis on impactful human studies. Such endeavors will inevitably require large-scale study execution and data management regulation (Big Biology, necessitating standardized and reliable metrics of immune status and function. A well-known example setting this large-scale effort in-motion is identifying correlations between eventual disease outcome and T lymphocyte phenotype in large HIV-patient cohorts using multiparameter flow cytometry. However, infection, immunodeficiency, and autoimmunity are also characterized by correlative and functional contributions of B lymphocytes, which to-date have received much less attention in the human Big Biology enterprise. Here, we review progress in human B cell phenotyping, analysis, and bioinformatics tools that constitute valuable resources for the B cell research community to effectively join in this effort.

  10. Human plasma cells express granzyme B.

    Science.gov (United States)

    Xu, Wei; Narayanan, Priya; Kang, Ning; Clayton, Sandra; Ohne, Yoichiro; Shi, Peiqing; Herve, Marie-Cecile; Balderas, Robert; Picard, Capucine; Casanova, Jean-Laurent; Gorvel, Jean-Pierre; Oh, Sangkon; Pascual, Virginia; Banchereau, Jacques

    2014-01-01

    While studying the plasma cell (PC) compartment in human tonsils, we identified that immunoglobulin kappa or lambda chain-expressing PCs are the main cells expressing granzyme B (GrzB). In vitro studies revealed that activated B cells differentiated into GrzB-expressing PCs when co-cultured with macrophages and follicular helper T cells. This effect could be reproduced on combined stimulation of IL-15 (produced by macrophages) and IL-21 (produced by T follicular helper cells) in a STAT3-dependent manner. Whereas IL-21 triggers the transcription of mRNA of GrzB, IL-15 synergizes the translation of GrzB proteins. The precise role of GrzB in PC biology remains to be understood and studies in mice will not help as their PCs do not express GrzB.

  11. Human CD56bright NK Cells

    DEFF Research Database (Denmark)

    Michel, Tatiana; Poli, Aurélie; Cuapio, Angelica

    2016-01-01

    Human NK cells can be subdivided into various subsets based on the relative expression of CD16 and CD56. In particular, CD56(bright)CD16(-/dim) NK cells are the focus of interest. They are considered efficient cytokine producers endowed with immunoregulatory properties, but they can also become...... cytotoxic upon appropriate activation. These cells were shown to play a role in different disease states, such as cancer, autoimmunity, neuroinflammation, and infection. Although their phenotype and functional properties are well known and have been extensively studied, their lineage relationship with other...... NK cell subsets is not fully defined, nor is their precise hematopoietic origin. In this article, we summarize recent studies about CD56(bright) NK cells in health and disease and briefly discuss the current controversies surrounding them....

  12. Human embryonic stem cells: preclinical perspectives

    Directory of Open Access Journals (Sweden)

    Sarda Kanchan

    2008-01-01

    Full Text Available Abstract Human embryonic stem cells (hESCs have been extensively discussed in public and scientific communities for their potential in treating diseases and injuries. However, not much has been achieved in turning them into safe therapeutic agents. The hurdles in transforming hESCs to therapies start right with the way these cells are derived and maintained in the laboratory, and goes up-to clinical complications related to need for patient specific cell lines, gender specific aspects, age of the cells, and several post transplantation uncertainties. The different types of cells derived through directed differentiation of hESC and used successfully in animal disease and injury models are described briefly. This review gives a brief outlook on the present and the future of hESC based therapies, and talks about the technological advances required for a safe transition from laboratory to clinic.

  13. Kvalitetssikring af Ul prober på baggrund af protokol til vurdering af tekniske fantom billeder

    DEFF Research Database (Denmark)

    Vernegreen, Susanne

    2013-01-01

    fordel sorteres fra i en revidering af protokollen. Jeg havde valgt at medtage dem, da de indgik i den oprindelige CIRS protokol, og det kan i en startfase være en fordel at have flere målinger at holde sig til. Min vurdering er dog at denne fordel ikke opvejer det øgede tidsforbrug. Der er dog nogle...... ikke muligt at undgå subjektivitet i forbindelse med måling og vurdering af de tekniske fantombilleder, da disse foretages manuelt. Det er derfor vigtigt at foretage flere målinger, og måske også oftere målinger end hvis målingerne var objektive. Flere og oftere målinger tager tid, og kan gå ud over...

  14. Stem cell factor and c-Kit in human primordial germ cells and fetal ovaries

    DEFF Research Database (Denmark)

    Høyer, Poul Erik; Byskov, Anne Grete; Møllgård, Kjeld

    2005-01-01

    Prenatal ovary (human), Primordial germ cells, Folliculogenesis, c-Kit, Stem cell factor, immunohistochemistry......Prenatal ovary (human), Primordial germ cells, Folliculogenesis, c-Kit, Stem cell factor, immunohistochemistry...

  15. A novel culture platform for fast proliferation of human annulus fibrosus cells.

    Science.gov (United States)

    Xiao, Li; Ding, Mengmeng; Saadoon, Osama; Vess, Eric; Fernandez, Andrew; Zhao, Ping; Jin, Li; Li, Xudong

    2017-02-01

    Tissue engineering provides a promising approach to treat degenerative disc disease, which usually requires a large quantity of seed cells. A simple and reliable in vitro culture system to expand seed cells in a timely fashion is necessary to implement the application clinically. Here, we sought to establish a cost-effective culture system for expanding human annulus fibrosus cells using extracellular matrix (ECM) proteins as culture substrates. Cells were cultured onto a plastic surface coated with various types of ECMs, including fibronectin, vitronectin, collagen type I, gelatin and cell-free matrix deposited by human nucleus pulposus cells. AF cell morphology, growth, adhesion and phenotype (anabolic and catabolic markers) were assessed by microscopy, real-time RT-PCR, western blotting, zymography, immunofluorescence staining and biochemical assays. Fibronectin, collagen and gelatin promoted cell proliferation and adhesion in a dose-dependent manner. Fibronectin elevated mRNA expression of proteoglycan and enhanced glycosaminoglycan production. Both collagen and gelatin increased protein expression of type II collagen. Consistent with increased cell adhesion, collagen and fibronectin promoted formation of focal adhesion complexes in the cell-matrix junction, suggesting enhanced binding of the actin network with both ECM substrates. On the other hand, fibronectin, collagen and gelatin decreased expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 in media. Finally, a mixture of fibronectin (1.7 μg/mL) and collagen (1.3 μg/mL) was identified as the most promising in vitro culture substrate system in promoting proliferation and maintaining anabolic-catabolic balance. Our method provides a simple and cost-effective platform for tissue engineering applications in intervertebral disc research.

  16. Forekomst af resistente bakterier og forbrug af antibiotika til hunde

    DEFF Research Database (Denmark)

    Pedersen, Karl; Pedersen, Kristina; Jensen, Helene;

    2007-01-01

    ), Pasteurella multocida (n=25), Bordetella bronchiseptica (n=14), Proteus spp. (n=29), og E. coli (n=449). I undersøgelsen anvendtes data fra VetStat databasen. Størstedelen af de antibiotika, der bruges til hunde er bredspektrede. Penicilliner med udvidet spektrum, cephalosporiner samt sulphonamider...... penicillin, 30,2% overfor fucidin og 27,9% overfor macrolider. E. coli isolaterne var mest resistente overfor ampicillin, sulphonamider, trimethoprim, tetracykliner og streptomycin, og anhæmolytiske isolater var oftere resistente overfor tetracykliner, trimethoprim og chloramphenikol end hæmolytiske isolater...

  17. Differences in the microrheology of human embryonic stem cells and human induced pluripotent stem cells.

    Science.gov (United States)

    Daniels, Brian R; Hale, Christopher M; Khatau, Shyam B; Kusuma, Sravanti; Dobrowsky, Terrence M; Gerecht, Sharon; Wirtz, Denis

    2010-12-01

    Embryonic and adult fibroblasts can be returned to pluripotency by the expression of reprogramming genes. Multiple lines of evidence suggest that these human induced pluripotent stem (hiPS) cells and human embryonic stem (hES) cells are behaviorally, karyotypically, and morphologically similar. Here we sought to determine whether the physical properties of hiPS cells, including their micromechanical properties, are different from those of hES cells. To this end, we use the method of particle tracking microrheology to compare the viscoelastic properties of the cytoplasm of hES cells, hiPS cells, and the terminally differentiated parental human fibroblasts from which our hiPS cells are derived. Our results indicate that although the cytoplasm of parental fibroblasts is both viscous and elastic, the cytoplasm of hiPS cells does not exhibit any measurable elasticity and is purely viscous over a wide range of timescales. The viscous phenotype of hiPS cells is recapitulated in parental cells with disassembled actin filament network. The cytoplasm of hES cells is predominantly viscous but contains subcellular regions that are also elastic. This study supports the hypothesis that intracellular elasticity correlates with the degree of cellular differentiation and reveals significant differences in the mechanical properties of hiPS cells and hES cells. Because mechanical stimuli have been shown to mediate the precise fate of differentiating stem cells, our results support the concept that stem cell "softness" is a key feature of force-mediated differentiation of stem cells and suggest there may be subtle functional differences between force-mediated differentiation of hiPS cells and hES cells.

  18. Autofluorescence of human cells in vitro as a biomarker of their metabolic activity

    Science.gov (United States)

    Dobrzyńska, Monika; Stepińska, Małgorzata; Lewandowski, Rafał; Gietka, Andrzej; Łapiński, Mariusz P.; Trafny, ElŻbieta A.

    2016-12-01

    Autofluorescence (AF) is the natural emission of light by intrinsic fluorophores. Oxidized mitochondrial flavins, lipofuscin and reduced nicotinamideadenine dinucleotide phosphate (NAD(P)H) are the main sources of the autofluorescence in cells upon excitation with visible light. The aim of the study was to investigate changes in the metabolism of four cell lines by monitoring their autofluorescence with a microplate reader. Autofluorescence intensities of cells were collected at two wavelengths for the excitation and fluorescence emission: for endogenous NAD(P)H at 366/450 nm, for the oxidized flavoproteins and lipofuscin at 460/540 nm. Human mesenchymal stem cells (hMSC), epithelial cells from mammary gland (MCF 10A), breast ductal carcinoma (T-47D) prostate carcinoma (DU-145) were observed daily for 16 days. The level of NAD(P)H autofluorescence did not differ among the cell lines investigated. The significant increase in oxidized flavoproteins fluorescence intensity was recorded for hMSC and ranged from 140 to 175% of control. During 28 days differentiation process, the NAD(P)H, FAD and lipofuscin fluorescence intensities were recorded every day, and the redox ratio was then calculated. The redox ratio gradually decreased during the last eight days of osteogenesis and adipogenesis. Therefore, in our opinion the NAD(P)H, FAD, and lipofuscin fluorescence emission at the wavelengths selected are the optimal parameters to be collected during the differentiation process in order to monitor the metabolism of hMSC undergoing structural and morphological changes.

  19. Generation of mature hematopoietic cells from human pluripotent stem cells.

    Science.gov (United States)

    Togarrati, Padma Priya; Suknuntha, Kran

    2012-06-01

    A number of malignant and non-malignant hematological disorders are associated with the abnormal production of mature blood cells or primitive hematopoietic precursors. Their capacity for continuous self-renewal without loss of pluripotency and the ability to differentiate into adult cell types from all three primitive germ layers make human embryonic stem cells and induced pluripotent stem cells (hiPSCs) attractive complementary cell sources for large-scale production of transfusable mature blood cell components in cell replacement therapies. The generation of patient-specific hematopoietic stem/precursor cells from iPSCs by the regulated manipulation of various factors involved in reprograming to ensure complete pluripotency, and developing innovative differentiation strategies for generating unlimited supply of clinically safe, transplantable, HLA-matched cells from hiPSCs to outnumber the inadequate source of hematopoietic stem cells obtained from cord blood, bone marrow and peripheral blood, would have a major impact on the field of regenerative and personalized medicine leading to translation of these results from bench to bedside.

  20. Human colostral cells. I. Separation and characterization.

    Science.gov (United States)

    Crago, S S; Prince, S J; Pretlow, T G; McGhee, J R; Mestecky, J

    1979-12-01

    Analyses of the cells present in human colostrum obtained from fifty-four healthy donors during the first four days of lactation revealed that there were 3.3 x 10(6) (range 1.1 x 10(5)--1.2 x 10(7)) cells per ml of colostrum. Based on histochemical examinations, it was found that this population consisted of 30--47% macrophages, 40--60% polymorphonuclear leucocytes, 5.2--8.9% lymphocytes, and 1.3--2.8% colostral corpuscles; epithelial cells were rarely encountered. The identity of various cell types was confirmed by Wright's stain and by a series of histochemical techniques which disclosed the presence of non-specific esterase, peroxidase, and lipids. For further characterization, the different types of cells were separated by various methods, such as Ficoll-Hypaque density centrifugation, isokinetic centrifugation on a linear Ficoll gradient, adherence to glass or plastic, and phagocytosis of carbonyl iron. Immunohistochemical staining with FITC- and/or TRITC-labelled reagents to IgA, IgM, IgG, K- and lambda-chains, secretory component, lactoferrin, and alpha-lactalbumin were applied to unseparated as well as separated colostral cells. Polymorphonuclear leucocytes (staining for peroxidase) as well as macrophages and colostral corpuscles (staining for non-specific esterase) exhibited numerous intracellular vesicles that contained lipids as well as various combinations of milk proteins. Lymphoid cells did not stain with any of these reagents and plasma cells were not detected among the colostral cells. Individual phagocytic cells contained immunoglobulins of the IgA and IgM classes, both K and lambda light chains, secretory component, lactoferrin, and alpha-lactalbumin. The coincidental appearance of these proteins in single, phagocytic cells but not in lymphoid cells indicate that the cells acquired these proteins by ingestion from the environment. Markers commonly used for the identification of B lymphocytes (surface immunoglobulins) and T lymphocytes (receptors

  1. 21 CFR 864.2280 - Cultured animal and human cells.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Cultured animal and human cells. 864.2280 Section... (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Cell And Tissue Culture Products § 864.2280 Cultured animal and human cells. (a) Identification. Cultured animal and human cells are in vitro...

  2. DNA repair responses in human skin cells

    Energy Technology Data Exchange (ETDEWEB)

    Hanawalt, P.C.; Liu, S.C.; Parsons, C.S.

    1981-07-01

    Sunlight and some environmental chemical agents produce lesions in the DNA of human skin cells that if unrepaired may interfere with normal functioning of these cells. The most serious outcome of such interactions may be malignancy. It is therefore important to develop an understanding of mechanisms by which the lesions may be repaired or tolerated without deleterious consequences. Our models for the molecular processing of damaged DNA have been derived largely from the study of bacterial systems. Some similarities but significant differences are revealed when human cell responses are tested against these models. It is also of importance to learn DNA repair responses of epidermal keratinocytes for comparison with the more extensive studies that have been carried out with dermal fibroblasts. Our experimental results thus far indicate similarities for the excision-repair of ultraviolet-induced pyrimidine dimers in human keratinocytes and fibroblasts. Both the monoadducts and the interstrand crosslinks produced in DNA by photoactivated 8-methoxypsoralen (PUVA) can be repaired in normal human fibroblasts but not in those from xeroderma pigmentosum patients. The monoadducts, like pyrimidine dimers, are probably the more mutagenic/carcinogenic lesions while the crosslinks are less easily repaired and probably result in more effective blocking of DNA function. It is suggested that a split-dose protocol that maximizes the production of crosslinks while minimizing the yield of monoadducts may be more effective and potentially less carcinogenic than the single ultraviolet exposure regimen in PUVA therapy for psoriasis.

  3. Human T Cell Memory: A Dynamic View

    Directory of Open Access Journals (Sweden)

    Derek C. Macallan

    2017-02-01

    Full Text Available Long-term T cell-mediated protection depends upon the formation of a pool of memory cells to protect against future pathogen challenge. In this review we argue that looking at T cell memory from a dynamic viewpoint can help in understanding how memory populations are maintained following pathogen exposure or vaccination. For example, a dynamic view resolves the apparent paradox between the relatively short lifespans of individual memory cells and very long-lived immunological memory by focussing on the persistence of clonal populations, rather than individual cells. Clonal survival is achieved by balancing proliferation, death and differentiation rates within and between identifiable phenotypic pools; such pools correspond broadly to sequential stages in the linear differentiation pathway. Each pool has its own characteristic kinetics, but only when considered as a population; single cells exhibit considerable heterogeneity. In humans, we tend to concentrate on circulating cells, but memory T cells in non-lymphoid tissues and bone marrow are increasingly recognised as critical for immune defence; their kinetics, however, remain largely unexplored. Considering vaccination from this viewpoint shifts the focus from the size of the primary response to the survival of the clone and enables identification of critical system pinch-points and opportunities to improve vaccine efficacy.

  4. Biological impact of human embryonic stem cells.

    Science.gov (United States)

    Martín, Miguel; Menéndez, Pablo

    2012-01-01

    Research on human embryonic stem cells (hESCs) and induced pluripotent (iPS) stem cells is currently a field of great potential in biomedicine. These cells represent a highly valuable tool for developmental biology studies, disease models, and drug screening and toxicity. The ultimate goal of hESCs and iPS cell research is the treatment of diseases or disorders for which there is currently no treatment or existing therapies are only partially effective. Despite the disproportionate short-term hopes generated, which are putting too much pressure on scientists, the international scientific community is making rapid progress in understanding hESCs and iPS cells. Nonetheless, great efforts have to be made to provide an answer to still quite basic questions concerning their biology. Moreover, translation to clinical applications in cell replacement therapy requires prior solution to ethical barriers. The recent development of iPS cells has provided a strong alternative to overcome ethical issues concerning hESCs. However, an in-depth characterization of their genetic and epigenetic features, as well as their differentiation potential still remains to be undertaken. This chapter will describe, precisely, what the critical issues are, where scientific and ethical barriers stand, and how we are to overcome them. Only then, we shall finally discover whether hESCs and iPS cells will allow building reproducible disease models, and whether they really are a safe tool, with great potential for regenerative medicine.

  5. Rat full term amniotic fluid harbors highly potent stem cells.

    Science.gov (United States)

    Mun-Fun, Hoo; Ferdaos, Nurfarhana; Hamzah, Siti Nurusaadah; Ridzuan, Noridzzaida; Hisham, Nurul Afiqah; Abdullah, Syahril; Ramasamy, Rajesh; Cheah, Pike See; Thilakavathy, Karrupiah; Yazid, Mohd Nazri; Nordin, Norshariza

    2015-10-01

    Amniotic fluid stem cells (AFSCs) are commonly isolated from mid-term amniotic fluid (AF) of animals and human collected via an invasive technique, amniocentesis. Alternatively, AFSCs could be collected at full-term. However, it is unclear whether AFSCs are present in the AF at full term. Here, we aimed to isolate and characterize stem cells isolated from AF of full term pregnant rats. Three stem cell lines have been established following immuno-selection against the stem cell marker, c-kit. Two of the new lines expressed multiple markers of pluripotency until more than passage 90. Further, they spontaneously differentiated into derivatives of the three primary germ layers through the formation of good quality embryoid bodies (EBs), and can be directly differentiated into neural lineage. Their strong stemness and potent neurogenic properties highlight the presence of highly potent stem cells in AF of full-term pregnancies, which could serve as a potential source of stem cells for regenerative medicine.

  6. Human fetal liver stromal cells expressing erythropoietin promote hematopoietic development from human embryonic stem cells.

    Science.gov (United States)

    Yang, Chao; Ji, Lei; Yue, Wen; Shi, Shuang-Shuang; Wang, Ruo-Yong; Li, Yan-Hua; Xie, Xiao-Yan; Xi, Jia-Fei; He, Li-Juan; Nan, Xue; Pei, Xue-Tao

    2012-02-01

    Blood cells transfusion and hematopoietic stem cells (HSCs) transplantation are important methods for cell therapy. They are widely used in the treatment of incurable hematological disorder, infectious diseases, genetic diseases, and immunologic deficiency. However, their availability is limited by quantity, capacity of proliferation and the risk of blood transfusion complications. Recently, human embryonic stem cells (hESCs) have been shown to be an alternative resource for the generation of hematopoietic cells. In the current study, we describe a novel method for the efficient production of hematopoietic cells from hESCs. The stable human fetal liver stromal cell lines (hFLSCs) expressing erythropoietin (EPO) were established using the lentiviral system. We observed that the supernatant from the EPO transfected hFLSCs could induce the hESCs differentiation into hematopoietic cells, especially erythroid cells. They not only expressed fetal and embryonic globins but also expressed the adult-globin chain on further maturation. In addition, these hESCs-derived erythroid cells possess oxygen-transporting capacity, which indicated hESCs could generate terminally mature progenies. This should be useful for ultimately developing an animal-free culture system to generate large numbers of erythroid cells from hESCs and provide an experimental model to study early human erythropoiesis.

  7. Cell phoney: human cloning after Quintavalle.

    Science.gov (United States)

    Morgan, Derek; Ford, Mary

    2004-12-01

    Reproductive cloning has thrown up new scientific possibilities, ethical conundrums, and legal challenges. An initial question, considered by the English courts in 2003, was whether the technique presently available, that of cell nucleus replacement, falls outside the provisions of the Human Fertilisation and Embryology Act 1990. If it does, the creation and use, including use in research protocols, of human embryos would be unregulated, disclosing a need to consider remedial legislation. The resolution by the courts of this legal question dramatically engages them in a resolution of fundamental ethical dilemmas, and discloses the possibilities and limitation of negotiating science policy through the processes of litigation.

  8. Gennemgang af den danske samfundsregulering

    DEFF Research Database (Denmark)

    Falch, Morten

    investeringer hos en bred række af markedsaktører’1. Midlet til at opnå dette mål har været at skabe et regelsæt der gør det lettere for nye virksomheder at komme ind på det nye telemarked, og konkurrere på lige fod med TDC. Herudover, har man hjulpet de nye operatører på vej gennem en såkaldt asymmetrisk...

  9. Etablering af global distribueret produktion

    DEFF Research Database (Denmark)

    Sørensen, Brian Vejrum; Johansen, John; Riis, Jens Ove

    2007-01-01

      Mange danske produktionsvirksomheder står over for en ny æra, som handler om at udvikle og styre globale distribuerede produktionsenheder og det netværk, disse til sammen danner. Interviews med top- og mellemledere i 15 danske produktionsvirksomheder med varierende størrelse og internationalt...... erfaringsgrundlag viser, at der i begyndelsen af globaliserings- og produktionsudflytningsprocessen isoleret set er stor fokus på omkostningsminimering - nogle gange kædet sammen med ønsker om markedsekspansion, men også at fokus og argumenterne ofte ændrer sig over tid og efterhånden bliver mere nuanceret. Ved...

  10. Lymphoid Cell-Glioma Cell Interaction Enhances Cell Coat Production by Human Gliomas: Novel Suppressor Mechanism

    Science.gov (United States)

    Dick, Steven J.; Macchi, Beatrice; Papazoglou, Savvas; Oldfield, Edward H.; Kornblith, Paul L.; Smith, Barry H.; Gately, Maurice K.

    1983-05-01

    Certain human glioma lines produce mucopolysaccharide coats that impair the generation of cytolytic lymphocytes in response to these lines in vitro. Coat production is substantially enhanced by the interaction of glioma cells with a macromolecular factor released by human peripheral blood mononuclear cells in culture. This interaction thus constitutes an unusual mechanism by which inflammatory cells may nonspecifically suppress the cellular immune response to at least one class of solid tumors in humans.

  11. Human induced pluripotent stem cell-derived models to investigate human cytomegalovirus infection in neural cells.

    Directory of Open Access Journals (Sweden)

    Leonardo D'Aiuto

    Full Text Available Human cytomegalovirus (HCMV infection is one of the leading prenatal causes of congenital mental retardation and deformities world-wide. Access to cultured human neuronal lineages, necessary to understand the species specific pathogenic effects of HCMV, has been limited by difficulties in sustaining primary human neuronal cultures. Human induced pluripotent stem (iPS cells now provide an opportunity for such research. We derived iPS cells from human adult fibroblasts and induced neural lineages to investigate their susceptibility to infection with HCMV strain Ad169. Analysis of iPS cells, iPS-derived neural stem cells (NSCs, neural progenitor cells (NPCs and neurons suggests that (i iPS cells are not permissive to HCMV infection, i.e., they do not permit a full viral replication cycle; (ii Neural stem cells have impaired differentiation when infected by HCMV; (iii NPCs are fully permissive for HCMV infection; altered expression of genes related to neural metabolism or neuronal differentiation is also observed; (iv most iPS-derived neurons are not permissive to HCMV infection; and (v infected neurons have impaired calcium influx in response to glutamate.

  12. Human somatic cell nuclear transfer and cloning.

    Science.gov (United States)

    2012-10-01

    This document presents arguments that conclude that it is unethical to use somatic cell nuclear transfer (SCNT) for infertility treatment due to concerns about safety; the unknown impact of SCNT on children, families, and society; and the availability of other ethically acceptable means of assisted reproduction. This document replaces the ASRM Ethics Committee report titled, "Human somatic cell nuclear transfer (cloning)," last published in Fertil Steril 2000;74:873-6. Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  13. Dopamine receptor repertoire of human granulosa cells

    Directory of Open Access Journals (Sweden)

    Kunz Lars

    2007-10-01

    Full Text Available Abstract Background High levels of dopamine (DA were described in human ovary and recently evidence for DA receptors in granulosa and luteal cells has been provided, as well. However, neither the full repertoire of ovarian receptors for DA, nor their specific role, is established. Human granulosa cells (GCs derived from women undergoing in vitro fertilization (IVF are an adequate model for endocrine cells of the follicle and the corpus luteum and were therefore employed in an attempt to decipher their DA receptor repertoire and functionality. Methods Cells were obtained from patients undergoing IVF and examined using cDNA-array, RT-PCR, Western blotting and immunocytochemistry. In addition, calcium measurements (with FLUO-4 were employed. Expression of two DA receptors was also examined by in-situ hybridization in rat ovary. Effects of DA on cell viability and cell volume were studied by using an ATP assay and an electronic cell counter system. Results We found members of the two DA receptor families (D1- and D2 -like associated with different signaling pathways in human GCs, namely D1 (as expected and D5 (both are Gs coupled and linked to cAMP increase and D2, D4 (Gi/Gq coupled and linked to IP3/DAG. D3 was not found. The presence of the trophic hormone hCG (10 IU/ml in the culture medium for several days did not alter mRNA (semiquantitative RT-PCR or protein levels (immunocytochemistry/Western blotting of D1,2,4,5 DA receptors. Expression of prototype receptors for the two families, D1 and D2, was furthermore shown in rat granulosa and luteal cells by in situ hybridization. Among the DA receptors found in human GCs, D2 expression was marked both at mRNA and protein levels and it was therefore further studied. Results of additional RT-PCR and Western blots showed two splice variants (D2L, D2S. Irrespective of these variants, D2 proved to be functional, as DA raised intracellular calcium levels. This calcium mobilizing effect of DA was observed

  14. Effekter af koordineret hjerneskaderehabilitering. Et systematisk litteraturreview

    DEFF Research Database (Denmark)

    Glintborg, Chalotte; Hansen, Tia G. B.

    Forskningsprojekt udvikler ny definition af begrebet koordination Som et led i forskningsprojektet ”Bio-psyko-sociale effekter af koordineret hjerneskaderehabilitering” er der udviklet en ny definition af koordinationsbegrebet. Forskningsprojektet ”Bio-psyko-sociale effekter af koordineret...... senhjerneskadeområdet – initiativer, der skal fremme en sammenhængende og helhedsorienteret rehabiliteringsindsats. Men hvad dækker begrebet koordination egentlig over? Det har et nyt forskningsprojekt set på og kommer med et bud på en ny definition. Dansk definition mangler Indholdet i koordination har været upræcist...... beskrevet. Der er ingen klar dansk definition af koordination. Begrebet bliver ofte brugt inkonsistent, og koordination er meget forskelligt tilrettelagt. Derfor kan man ikke generelt dokumentere, om koordinatorfunktioner er en sikker vej til forbedring af komplekse rehabiliteringsforløb. Sundhedsstyrelsen...

  15. DK-SOFCa. Development of solid oxide fuel cells. Technical final report; DK-SOFCa, udvikling af fastoxid braendselsceller. Teknisk slutrapport

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, P.H.

    2004-08-01

    This project is part of the Danish strategy for solid oxide fuel cells. The main purpose with the project was to upgrade Danish fuel cell technology within the SOFC area from established laboratory production techniques to pre-pilot scale. This target has been reached, as a 400m{sup 2} production area with equipment capable of producing up to 500 homogeneous, batch-size cells with a low rejection rate has been established. The production processes are described, analysed and optimised systematically in order to create a basis for a future upgrading to industrial production. A number of non-destructive evaluation methods have been developed for quality control of the produced cells. A reproducibility study of the established production process has contributed to identifying reasons for variations in the yield of the cells, and has enabled a continuous quality improvement. The cells produced on the pre-pilot plant are globally competitive as to mechanical strength, service life and electrical yield. Thus, the project has opened an opportunity for a demonstration of Danish fuel cell technology. (BA)

  16. TALEN-Induced Translocations in Human Cells.

    Science.gov (United States)

    Piganeau, Marion; Renouf, Benjamin; Ghezraoui, Hind; Brunet, Erika

    2016-01-01

    Induction of chromosomal translocations in human cells is of a great interest to study tumorigenesis and genome instability. Here, we explain in detail a method to induce translocations using the transcription activator-like effector nucleases (TALENs). We describe how to detect translocation formation by PCR, calculate translocation frequency by 96-well PCR screen, and analyze breakpoint junctions. When inducing cancer translocations, it is also possible to detect the fusion gene by FISH analysis or western blot.

  17. Danskernes brug af nyhedsmedier: et nyt landkort

    DEFF Research Database (Denmark)

    Schrøder, Kim Christian

    2010-01-01

    Artiklen fremlægger første del af en analyse, der på en ny måde kortlægger danskernes brug og oplevelse af nyheder i det medialiserede samfund, på tværs af de forskellige – traditionelle og digitale - medieplatforme og genrer. Teoretisk er undersøgelsen forankret i offentlighedsteorien og den beg...

  18. Praktik - set i lyset af billedkunstfaget

    DEFF Research Database (Denmark)

    Buhl, Mie; Skov, Kirsten; Meisner Christensen, Kirsten

    Kamelgruppen har vi været optaget af at pege på relevante temaer for udvikling af billedfaget og forandring i uddannelsen af kommende lærere. Foråret 2004 præsenterede gruppens medlemmer papers på symposiet 'Visual culture in education' på kongressen på Nordisk Forening for Pædagogisk forskning i Reykjavik...

  19. Konceptuelt design og udvikling af Intergomus

    DEFF Research Database (Denmark)

    Ellebæk, P.; Pedersen, T.; Stendal, Karsten

    1999-01-01

    Denne rapport gennemgår udviklingsforløbet af et træningsapparat til genoptræning af læderede fingre. Rapporten omhandler kun den mekaniske del af udstyret. Opgaven er blevet løst i samarbejde med Ergoterapien på Køge Amtssygehus, Procare, Centret forAvanceret Teknologi og flere afdelinger på RIS...

  20. Refleksioner over udarbejdelse af tests i Blackboard

    DEFF Research Database (Denmark)

    Grinsted, Annelise

    2006-01-01

    2 lektorer, der indgår i et pædagogisk udviklingsarbejde som en del af e-læringsstrategien på Syddansk Universitet, skal udvikle 3 former for tests i universitetets valgte læringssystem, Blackboard, afprøve disse og derefter evaluere på resultaterne af denne proces. Udarbejdelsen af spørgsmål i...

  1. Metodebeskrivelse for evaluering af arkitektonisk kvalitet

    DEFF Research Database (Denmark)

    Beim, Anne

    2000-01-01

    Artiklen indgår i en større publikation som er en vejledning i evaluering af boligbebyggelsers standard og kvalitet. Vejledningen er bygget op over et evalueringskoncept, der primært er tilrettelagt med henblik på evaluering af (almene) boligbebyggelser og er udarbejdet til vurdering af 10...... forsøgsbyggerier inden for Erhvervsfremmestyrelsens og By - Boligministeriets udviklingsinitiativ ' Proces og Produktudvikling i byggeriet' - det såkaldte PPB- program....

  2. Offentliggørelse af kursrelevante oplysninger

    DEFF Research Database (Denmark)

    Werlauff, Erik; Schaumburg-Müller, Peer

    2013-01-01

    Artiklen analyserer, særligt på baggrund af EU-Domstolens dom af 28. juni 2012 i C-19/11, Daimler AG, hvilken betydning en række mellemliggende trin, der fører frem til en kursrelevant og offentliggørelsespligtig begivenhed, har for tidspunktet for indtrædelsen af pligten til offentliggørelse. De...

  3. En kritisk analyse af Leviathan & Samfundspagten

    OpenAIRE

    Bjelobrk, Aleksandar

    2006-01-01

    I denne rapport undersøges de politiske filosoffer Thomas Hobbes’ (1588-1679) og Jean-Jacques Rousseaus (1712-1778) udgaver af kontraktteorien. Med udgangspunkt i Leviathan del 1, Of Man og del 2, Of Common-Wealth af Thomas Hobbes (Hobbes 1991, oprindelig udgivet 1651) og Samfundspagten af Jean-Jacques Rousseau (Rousseau 1987, oprindelig udgivet 1762) klarlægges inkonsisten i deres teorier gennem to separate analyser. Med udgangspunkt i den fundne inkonsistens foretages en komparativ analyse,...

  4. Afgivelse af formaldehyd fra byggevarer og forbrugerprodukter

    DEFF Research Database (Denmark)

    Kolarik, Barbara; Gunnarsen, Lars; Funch, Llis Winther

    Rapporten præsenterer resultatet af en undersøgelse af formaldehydafgivelse fra 22 mulige kilder i indeklimaet. Undersøgelsen er gennemført for om muligt at finde årsagen til de høje koncentrationer af formaldehyd i indeluften, der blev fundet i enkelte danske boliger under en foregående undersøg...

  5. Motivation af den Kreative Sælger

    OpenAIRE

    Sørensen, Mikkel Vallentin; Floor, Jeppe Hjelmsted

    2012-01-01

    Projektet tager udgangspunkt i motivationen af danske sælgere. Med inspiration fra den positive psykologiske videnskab, udfordres eksisterende motivationsredskaber, med henblik på at motivere den enkelte medarbejder til at yde et bedre stykke arbejde for virksomheden. Daniel Pink, Bruno Frey og Frederick Herzberg danner det teoretiske grundlag for opgaven, og problemstillingen undersøges gennem en sammenholdelse af deres teorier med empirisk materiale i en fænomenologisk tilgang. Rapporten af...

  6. The core regulatory network in human cells.

    Science.gov (United States)

    Kim, Man-Sun; Kim, Dongsan; Kang, Nam Sook; Kim, Jeong-Rae

    2017-03-04

    In order to discover the common characteristics of various cell types in the human body, many researches have been conducted to find the set of genes commonly expressed in various cell types and tissues. However, the functional characteristics of a cell is determined by the complex regulatory relationships among the genes rather than by expressed genes themselves. Therefore, it is more important to identify and analyze a core regulatory network where all regulatory relationship between genes are active across all cell types to uncover the common features of various cell types. Here, based on hundreds of tissue-specific gene regulatory networks constructed by recent genome-wide experimental data, we constructed the core regulatory network. Interestingly, we found that the core regulatory network is organized by simple cascade and has few complex regulations such as feedback or feed-forward loops. Moreover, we discovered that the regulatory links from genes in the core regulatory network to genes in the peripheral regulatory network are much more abundant than the reverse direction links. These results suggest that the core regulatory network locates at the top of regulatory network and plays a role as a 'hub' in terms of information flow, and the information that is common to all cells can be modified to achieve the tissue-specific characteristics through various types of feedback and feed-forward loops in the peripheral regulatory networks. We also found that the genes in the core regulatory network are evolutionary conserved, essential and non-disease, non-druggable genes compared to the peripheral genes. Overall, our study provides an insight into how all human cells share a common function and generate tissue-specific functional traits by transmitting and processing information through regulatory network.

  7. Cell Culture Assay for Human Noroviruses [response

    Energy Technology Data Exchange (ETDEWEB)

    Straub, Tim M.; Honer Zu Bentrup, Kerstin; Orosz Coghlan, Patricia; Dohnalkova, Alice; Mayer, Brooke K.; Bartholomew, Rachel A.; Valdez, Catherine O.; Bruckner-Lea, Cindy J.; Gerba, Charles P.; Abbaszadegan, Morteza A.; Nickerson, Cheryl A.

    2007-07-01

    We appreciate the comments provided by Leung et al., in response to our recently published article “In Vitro Cell Culture Infectivity Assay for Human Noroviruses” by Straub et al. (1). The specific aim of our project was to develop an in vitro cell culture infectivity assay for human noroviruses (hNoV) to enhance risk assessments when they are detected in water supplies. Reverse transcription (RT) qualitative or quantitative PCR are the primary assays for waterborne NoV monitoring. However, these assays cannot distinguish between infectious vs. non-infectious virions. When hNoV is detected in water supplies, information provided by our infectivity assay will significantly improve risk assessment models and protect human health, regardless of whether we are propagating NoV. Indeed, in vitro cell culture infectivity assays for the waterborne pathogen Cryptosporidium parvum that supplement approved fluorescent microscopy assays, do not result in amplification of the environmentally resistant hard-walled oocysts (2). However, identification of life cycle stages in cell culture provides evidence of infectious oocysts in a water supply. Nonetheless, Leung et al.’s assertion regarding the suitability of our method for the in vitro propagation of high titers of NoV is valid for the medical research community. In this case, well-characterized challenge pools of virus would be useful for developing and testing diagnostics, therapeutics, and vaccines. As further validation of our published findings, we have now optimized RT quantitative PCR to assess the level of viral production in cell culture, where we are indeed finding significant increases in viral titer. The magnitude and time course of these increases is dependent on both virus strain and multiplicity of infection. We are currently preparing a manuscript that will discuss these findings in greater detail, and the implications this may have for creating viral challenge pools

  8. Preliminary study on human fibroblasts as feeder layer for human embryonic stem cells culture in vitro

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    To avoid the direct contact with mouse cells and possible heterogeneous pathogen in future application, we need to replace mouse embryonic fibroblastswith human fibroblasts as the feeder layer to maintain human embryonic stem cells growth in the undifferentiated state. We successfully use human fibroblasts derived from aborted fetus and adult prepuce as feeder layer to maintain human embryonic stem cells growth. During the passage and growth on this feeder layer, the human embryonic stem cells can keep their undifferentiated state.

  9. Prøvetagning af grundvand

    DEFF Research Database (Denmark)

    Sørensen, Inga

    Bogen fokuserer på prøveatgning af grundvand og drikkevand til forskellige formål såsom undersøgelse af forurenede grunde, grundvandsmonitering samt produktion af drikkevand. Da grundvand i sagens natur findes under jordoverfladen, beskriver bogen også væsentlige forhold om hydrogeologi og grundv...... grundvandskemi i det omfang, disse forhold har betydning for den praktiske prøvetagning. Der redegøres også for de forskellige typer af pumper og udstyr samt emballage til vandprøver inklusive fordele og ulemer ved de forskellige typer....

  10. Omsætning af fast ejendom

    DEFF Research Database (Denmark)

    Edlund, Hans Henrik

    Bogen er en systematisk gennemgang af lov om omsætning af fast ejendom, der regulerer ejendomsmægleres virke. Følgende emner er bl.a. behandlet: Hvem kan drive ejendomsmæglervirksomhed og ejendomsmæglernes pligter og ansvar.......Bogen er en systematisk gennemgang af lov om omsætning af fast ejendom, der regulerer ejendomsmægleres virke. Følgende emner er bl.a. behandlet: Hvem kan drive ejendomsmæglervirksomhed og ejendomsmæglernes pligter og ansvar....

  11. Review af arbejdsmarkedsstyrelsen survey om rekruttering

    DEFF Research Database (Denmark)

    Bach, Henning Bjerregaard; Milhøj, Anders

    Arbejdsmarkedsstyrelsen har bestilt konsulentfirmaet Rambøll Management til to gange årligt at gennemføre en survey om danske virksomheders rekruttering af medarbejdere. I dette review gennemgår og vurderer SFI surveyens design, herunder udformningen af spørgeskemaer og vejledninger, dataindsamli......Arbejdsmarkedsstyrelsen har bestilt konsulentfirmaet Rambøll Management til to gange årligt at gennemføre en survey om danske virksomheders rekruttering af medarbejdere. I dette review gennemgår og vurderer SFI surveyens design, herunder udformningen af spørgeskemaer og vejledninger...

  12. NIR optimerer produktionen af gammeldags modnede sild

    DEFF Research Database (Denmark)

    Svensson, Vibeke Tølbøl; Bro, Rasmus; Nielsen, Henrik Hauch;

    2005-01-01

    Måling med nærinfrarødt (NIR) lys er et godt supplement til de nuværende metoder til at følge modningen af sild saltede i tønder. Det viser resultaterne af et forskningsprojekt udført i samarbejde mellem Lykkeberg A/S, Danmarks Fiskeriundersøgelser og Den Kgl Veterinær- og Landbohøjskole. Ved hjælp...... af avanceret matematik er det nemt og hurtigt at bestemme modningsgraden af sild direkte fra en NIR måling....

  13. Opstilling af ventilationsarmaturer i CFD-beregninger

    DEFF Research Database (Denmark)

    Nielsen, Peter V.

    2009-01-01

    Opstilling af armaturer i CFD-beregninger er et område, hvor ventilationsbranchen skal tilføre CFD-metoderne en specialviden, som ikke på forhånd er indlagt i programmerne. I artiklen bringes nogle eksempler på specificering af en indblæsningsåbning......Opstilling af armaturer i CFD-beregninger er et område, hvor ventilationsbranchen skal tilføre CFD-metoderne en specialviden, som ikke på forhånd er indlagt i programmerne. I artiklen bringes nogle eksempler på specificering af en indblæsningsåbning...

  14. Primitive cardiac cells from human embryonic stem cells.

    Science.gov (United States)

    Hudson, James; Titmarsh, Drew; Hidalgo, Alejandro; Wolvetang, Ernst; Cooper-White, Justin

    2012-06-10

    Pluripotent stem cell-derived cardiomyocytes are currently being investigated for in vitro human heart models and as potential therapeutics for heart failure. In this study, we have developed a differentiation protocol that minimizes the need for specific human embryonic stem cell (hESC) line optimization. We first reduced the heterogeneity that exists within the starting population of bulk cultured hESCs by using cells adapted to single-cell passaging in a 2-dimensional (2D) culture format. Compared with bulk cultures, single-cell cultures comprised larger fractions of TG30(hi)/OCT4(hi) cells, corresponding to an increased expression of pluripotency markers OCT4 and NANOG, and reduced expression of early lineage-specific markers. A 2D temporal differentiation protocol was then developed, aimed at reducing the inherent heterogeneity and variability of embryoid body-based protocols, with induction of primitive streak cells using bone morphogenetic protein 4 and activin A, followed by cardiogenesis via inhibition of Wnt signaling using the small molecules IWP-4 or IWR-1. IWP-4 treatment resulted in a large percentage of cells expressing low amounts of cardiac myosin heavy chain and expression of early cardiac progenitor markers ISL1 and NKX2-5, thus indicating the production of large numbers of immature cardiomyocytes (~65,000/cm(2) or ~1.5 per input hESC). This protocol was shown to be effective in HES3, H9, and, to a lesser, extent, MEL1 hESC lines. In addition, we observed that IWR-1 induced predominantly atrial myosin light chain (MLC2a) expression, whereas IWP-4 induced expression of both atrial (MLC2a) and ventricular (MLC2v) forms. The intrinsic flexibility and scalability of this 2D protocol mean that the output population of primitive cardiomyocytes will be particularly accessible and useful for the investigation of molecular mechanisms driving terminal cardiomyocyte differentiation, and potentially for the future treatment of heart failure.

  15. Appearance of Human Plasma Cells Following Differentiation of Human B Cells in NOD/SCID Mouse Spleen

    OpenAIRE

    2003-01-01

    Relatively little is known for the differentiation and maturation process of human B cells to plasma cells. This is particularly important in reconstitution work involving transfer of autoantibodies. To address this issue, we transplanted human peripheral blood mononuclear cells (PBMC) directly into the spleen of irradiated NOD/SCID mice depleted of natural killer cell activity. Within 6 weeks, naïve B cells differentiated into memory B cells and, importantly, the numbers of human CD138+ plas...

  16. Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

    Directory of Open Access Journals (Sweden)

    Hayato Fukusumi

    2016-01-01

    Full Text Available Human neural progenitor cells (hNPCs have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi. Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

  17. Human microglial cells synthesize albumin in brain.

    Directory of Open Access Journals (Sweden)

    Sung-Min Ahn

    Full Text Available Albumin, an abundant plasma protein with multifunctional properties, is mainly synthesized in the liver. Albumin has been implicated in Alzheimer's disease (AD since it can bind to and transport amyloid beta (Abeta, the causative agent of AD; albumin is also a potent inhibitor of Abeta polymerization. Despite evidence of non-hepatic transcription of albumin in many tissues including kidney and pancreas, non-hepatic synthesis of albumin at the protein level has been rarely confirmed. In a pilot phase study of Human Brain Proteome Project, we found evidence that microglial cells in brain may synthesize albumin. Here we report, for the first time, the de novo synthesis of albumin in human microglial cells in brain. Furthermore, we demonstrate that the synthesis and secretion of albumin from microglial cells is enhanced upon microglial activation by Abeta(1-42- or lipopolysaccharide (LPS-treatment. These data indicate that microglial cells may play a beneficial role in AD by secreting albumin that not only inhibits Abeta polymerization but also increases its clearance.

  18. Neocortical glial cell numbers in human brains

    DEFF Research Database (Denmark)

    Pelvig, D.P.; Pakkenberg, H.; Stark, A.K.

    2008-01-01

    and neurons and counting were done in each of the four lobes. The study showed that the different subpopulations of glial cells behave differently as a function of age; the number of oligodendrocytes showed a significant 27% decrease over adult life and a strong correlation to the total number of neurons...... while the total astrocyte number is constant through life; finally males have a 28% higher number of neocortical glial cells and a 19% higher neocortical neuron number than females. The overall total number of neocortical neurons and glial cells was 49.3 billion in females and 65.2 billion in males......, a difference of 24% with a high biological variance. These numbers can serve as reference values in quantitative studies of the human neocortex. (C) 2007 Elsevier Inc. All rights reserved Udgivelsesdato: 2008/11...

  19. Characterizing motility dynamics in human RPE cells

    Science.gov (United States)

    Liu, Zhuolin; Kurokawa, Kazuhiro; Zhang, Furu; Miller, Donald T.

    2017-02-01

    Retinal pigment epithelium (RPE) cells are vital to health of the outer retina, however, are often compromised in ageing and ocular diseases that lead to blindness. Early manifestation of RPE disruption occurs at the cellular level, but while in vivo biomarkers at this scale hold considerable promise, RPE cells have proven extremely challenging to image in the living human eye. Recently we addressed this problem by using organelle motility as a novel contrast agent to enhance the RPE cell in conjunction with 3D resolution of adaptive optics-optical coherence tomography (AO-OCT) to section the RPE layer. In this study, we expand on the central novelty of our method - organelle motility - by characterizing the dynamics of the motility in individual RPE cells, important because of its direct link to RPE physiology. To do this, AO-OCT videos of the same retinal patch were acquired at approximately 1 min intervals or less, time stamped, and registered in 3D with sub-cellular accuracy. Motility was quantified by an exponential decay time constant, the time for motility to decorrelate the speckle field across an RPE cell. In two normal subjects, we found the decay time constant to be just 3 seconds, thus indicating rapid motility in normal RPE cells.

  20. Human cell culture in a space bioreactor

    Science.gov (United States)

    Morrison, Dennis R.

    1988-01-01

    Microgravity offers new ways of handling fluids, gases, and growing mammalian cells in efficient suspension cultures. In 1976 bioreactor engineers designed a system using a cylindrical reactor vessel in which the cells and medium are slowly mixed. The reaction chamber is interchangeable and can be used for several types of cell cultures. NASA has methodically developed unique suspension type cell and recovery apparatus culture systems for bioprocess technology experiments and production of biological products in microgravity. The first Space Bioreactor was designed for microprocessor control, no gaseous headspace, circulation and resupply of culture medium, and slow mixing in very low shear regimes. Various ground based bioreactors are being used to test reactor vessel design, on-line sensors, effects of shear, nutrient supply, and waste removal from continuous culture of human cells attached to microcarriers. The small Bioreactor is being constructed for flight experiments in the Shuttle Middeck to verify systems operation under microgravity conditions and to measure the efficiencies of mass transport, gas transfer, oxygen consumption and control of low shear stress on cells.

  1. Snit af målinger: VK afhængig af DF og NA

    DEFF Research Database (Denmark)

    Thomsen, Søren Risbjerg

    2007-01-01

    ANALYSE: Ekstra Risbjerg-snit af meningsmålinger grundet fortsat valgrummel - VK-regeringen vil blive afhængig af både DF og NA. Udgivelsesdato: 21. august 2007......ANALYSE: Ekstra Risbjerg-snit af meningsmålinger grundet fortsat valgrummel - VK-regeringen vil blive afhængig af både DF og NA. Udgivelsesdato: 21. august 2007...

  2. Hypervirkelig(?). Studier af ungdom, skole og modernitet i udkanten af Kathmandu

    DEFF Research Database (Denmark)

    Madsen, Ulla Ambrosius

    2010-01-01

    Med udgangspunkt i etnografiske studier af ungdom og skoleliv i udkanten af Kathmandu, analyserer artiklen samspillet mellem 'det moderne', uddannelse og identitet blandt unge der lever i udkanten af den globale periferi.I modsætning til studier der beskæftiger sig med om og hvordan uddannelse bi...... fremtiden. Teoretisk trækker deres på begreber og positioner fra postmoderne metodologiskeog filosofiske orienteringer - repræsenteret blandt andre af Popkewitz, Maclure og Baudrillard....

  3. Appearance of Human Plasma Cells Following Differentiation of Human B Cells in NOD/SCID Mouse Spleen

    Directory of Open Access Journals (Sweden)

    Kentaro Kikuchi

    2003-01-01

    Full Text Available Relatively little is known for the differentiation and maturation process of human B cells to plasma cells. This is particularly important in reconstitution work involving transfer of autoantibodies. To address this issue, we transplanted human peripheral blood mononuclear cells (PBMC directly into the spleen of irradiated NOD/SCID mice depleted of natural killer cell activity. Within 6 weeks, naïve B cells differentiated into memory B cells and, importantly, the numbers of human CD138+ plasma cells in spleen increased by 100 fold after transplantation. Plasma cell numbers correlated with the detection of human IgM and IgG in serum, indicating that human B cells had differentiated into mature plasma cells in the murine spleen. In addition to CD19+ plasma cells, a distinct CD19- plasma cell population was detected, suggesting that downregulation of CD19 associated with maturation of plasma cells occurred. When purified human B cells were transplanted, those findings were not observed. Our results indicate that differentiation and maturation of human B cells and plasma cells can be investigated by transplantation of human PBMC into the spleen of NOD/SCID mice. The model will be useful for studying the differentiation of human B cells and generation of plasma cells.

  4. Midler til lindring af mundtørhed og stimulation af spytsekretion

    DEFF Research Database (Denmark)

    Dynesen, Anja Weirsøe; Pedersen, Anne Marie Lynge; Jensen, Allan Bardow;

    2013-01-01

    effekt. I relation til risikoen for at udvikle caries, der er forhøjet hos den mundtørre patient, er det dog væsentligt at anbefale produkter uden sukker. Ligesom produktets indhold af syre og dermed det erosive potentiale er af betydning i forhold til udviklingen af dentale erosioner. Systemisk...

  5. Human somatic cell nuclear transfer is alive and well.

    Science.gov (United States)

    Cibelli, Jose B

    2014-06-05

    In this issue, Chung et al. (2014) generate human embryonic stem cells by fusing an adult somatic cell to a previously enucleated human oocyte, in agreement with recent reports by the Mitalipov and Egli groups. We can now safely say that human somatic cell nuclear transfer is alive and well.

  6. Life cycle assessment of micro production technologies - solar cells, household wind turbines and micro motors; Livscyklusvurdering af mikroproduktionsteknologier - solceller, husstandsvindmoeller og mikromotorer

    Energy Technology Data Exchange (ETDEWEB)

    Faeborg Poulsen, A.; Kruse, H.; Hvid Ipsen, K.

    2000-07-15

    This report contains a life cycle based estimation of environmental effects of CHP based on the micro production technologies: solar cells, household wind turbines and micro motors. The technologies cover CHP production on very small plants which are characterised by being located by the individual consumer. (BA)

  7. Effects of c-myc oncogene modulation on differentiation of human small cell lung carcinoma cell lines

    NARCIS (Netherlands)

    Van Waardenburg, RCAM; Meijer, C; Pinto-Sietsma, SJ; De Vries, EGE; Timens, W; Mulder, NM

    1998-01-01

    Amplification and over-expression of oncogenes of the myc family are related to the prognosis of certain solid tumors such as small cell lung cancer (SCLC). For SCLC, c-myc is the oncogene most consistently found to correlate with the end stage behaviour of the tumour, in particular with survival af

  8. The association between human papillomavirus and oropharyngeal squamous cell Carcinoma

    DEFF Research Database (Denmark)

    Walvik, Lena; Svensson, Amanda Björk; Friborg, Jeppe

    2016-01-01

    There is emerging evidence of the association between human papillomavirus and a subset of head and neck cancers. However, the role of human papillomavirus as a causal factor is still debated. This review addresses the association between human papillomavirus and oropharyngeal squamous cell...... of well-defined premalignant lesions. However, a causal relationship between human papillomavirus infection and oropharyngeal squamous cell carcinoma seems evident....

  9. Barrierer for ibrugtagning af videooptaget universitetsundervisning

    Directory of Open Access Journals (Sweden)

    Pernille Rattleff

    2009-06-01

    Full Text Available Danmarks Pædagogiske Universitetsskole ved Aarhus Universitet har siden 2006 eksperimenteret med systematisk videooptagelse og -streamning af undervisning på udvalgte kandidat- og masteruddannelser. De studerende er begejstrede for denne praksis og de muligheder, dette giver de studerende for at understøtte deres læring. Men selvom de studerende har vist sig at bruge den videostreamede undervisning på en endog særdeles hensigtsmæssig måde, har der, både blandt studerende og især blandt underviserne, været modstand mod dels overhovedet at optage, dels at gøre optagelserne af undervisningen offentligt tilgængelige for studerende og andre interesserede. Der kan således iagttages nogle barrierer i forhold til ibrugtagning af videooptaget undervisning på universitetsniveau, idet underviserne for en umiddelbar betragtning tilsyneladende for det første har nogle forbehold af psykologisk, for det andet af juridisk karakter og for det tredje af organisatorisk art. Et af de centrale spørgsmål i denne forbindelse synes således at være, om optagelse af undervisning muliggør en ledelsesmæssig evaluering af den enkelte undervisers undervisningspraksis. Et andet centralt spørgsmål er tilsyneladende, om optagelse af undervisningen, der muliggør genbrug, gør underviserne overflødige. Forfatterne til denne artikel er imidlertid af den opfattelse, at undervisernes modstand følger af en anden, og mere grundlæggende, diskrepans, nemlig diskrepansen mellem det at forske og det at undervise. Efter en indledende begrebsafklaring, præsenterer forfatterne i denne artikel erfaringerne med tilbud af videostreamet og podcastet universitetsundervisning til studerende på Danmarks Pædagogiske Universitetsskole med henblik på en diskussion af de potentialer og især de barrierer, der kan iagttages i forhold til en mere systematisk, large-scale brug af castet universitetsundervisning. I forlængelse heraf formulerer forfatterne desuden en mulig

  10. Cell cycle regulation in human embryonic stem cells: links to adaptation to cell culture.

    Science.gov (United States)

    Barta, Tomas; Dolezalova, Dasa; Holubcova, Zuzana; Hampl, Ales

    2013-03-01

    Cell cycle represents not only a tightly orchestrated mechanism of cell replication and cell division but it also plays an important role in regulation of cell fate decision. Particularly in the context of pluripotent stem cells or multipotent progenitor cells, regulation of cell fate decision is of paramount importance. It has been shown that human embryonic stem cells (hESCs) show unique cell cycle characteristics, such as short doubling time due to abbreviated G1 phase; these properties change with the onset of differentiation. This review summarizes the current understanding of cell cycle regulation in hESCs. We discuss cell cycle properties as well as regulatory machinery governing cell cycle progression of undifferentiated hESCs. Additionally, we provide evidence that long-term culture of hESCs is accompanied by changes in cell cycle properties as well as configuration of several cell cycle regulatory molecules.

  11. Glycomics of human embryonic stem cells and human induced pluripotent stem cells.

    Science.gov (United States)

    Furukawa, Jun-Ichi; Okada, Kazue; Shinohara, Yasuro

    2016-10-01

    Most cells are coated by a dense glycocalyx composed of glycoconjugates such as glycosphingolipids, glycoproteins, and proteoglycans. The overall glycomic profile is believed to be crucial for the diverse roles of glycans, which are mediated by specific interactions that regulate cell-cell adhesion, the immune response, microbial pathogenesis, and other cellular events. Many cell surface markers were discovered and identified as glycoconjugates such as stage-specific embryonic antigen, Tra-1-60/81 and various other cell surface molecules (e.g., cluster of differentiation). Recent progress in the development of analytical methodologies and strategies has begun to clarify the cellular glycomics of various cells including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). The glycomic profiles of these cells are highly cell type-specific and reflect cellular alterations, such as development, differentiation and cancerous change. In this mini review, we briefly summarize the glycosylation spectra specific to hESCs and hiPSCs, which cover glycans of all major glycoconjugates (i.e., glycosphingolipids, N- and O-glycans of glycoproteins, and glycosaminoglycans) and free oligosaccharides.

  12. Isolation and characterization of human spermatogonial stem cells

    Directory of Open Access Journals (Sweden)

    Liu Shixue

    2011-10-01

    Full Text Available Abstract Background To isolate and characterization of human spermatogonial stem cells from stem spermatogonium. Methods The disassociation of spermatogonial stem cells (SSCs were performed using enzymatic digestion of type I collagenase and trypsin. The SSCs were isolated by using Percoll density gradient centrifugation, followed by differential surface-attachment method. Octamer-4(OCT4-positive SSC cells were further identified using immunofluorescence staining and flow cytometry technques. The purity of the human SSCs was also determined, and a co-culture system for SSCs and Sertoli cells was established. Results The cell viability was 91.07% for the suspension of human spermatogonial stem cells dissociated using a two-step enzymatic digestion process. The cells isolated from Percoll density gradient coupled with differential surface-attachement purification were OCT4 positive, indicating the cells were human spermatogonial stem cells. The purity of isolated human spermatogonial stem cells was 86.7% as assessed by flow cytometry. The isolated SSCs were shown to form stable human spermatogonial stem cell colonies on the feeder layer of the Sertoli cells. Conclusions The two-step enzyme digestion (by type I collagenase and trypsin process is an economical, simple and reproducible technique for isolating human spermatogonial stem cells. With little contamination and less cell damage, this method facilitates isolated human spermatogonial stem cells to form a stable cell colony on the supporting cell layer.

  13. Optofluidic Waveguides in Teflon AF-Coated PDMS Microfluidic Channels.

    Science.gov (United States)

    Cho, Sung Hwan; Godin, Jessica; Lo, Yu-Hwa

    2009-08-01

    We report a new method for fabricating an optofluidic waveguide that is compatible with polydimethylsiloxane (PDMS). The light path follows the microfluidic channels, an architecture that can maximize detection efficiency and make the most economic use of chip area in many lab-on-chip applications. The PDMS-based microfluidic channels are coated with Teflon amorphous fluoropolymers (Teflon AF) which has a lower refractive index (n = 1.31) than water (n = 1.33) to form a water/Teflon AF optical waveguide. Driven by a vacuum pump, the Teflon AF solution was flowed through the channels, leaving a thin (5-15 µm) layer of coating on the channel wall as the cladding layer of optical waveguides. This coating process resolves the limitations of spin-coating processes by reducing the elasticity mismatch between the Teflon AF cladding layer and the PDMS device body. We demonstrate that the resulting optofluidic waveguide confines and guides the laser light through the liquid core channel. Furthermore, the light in such a waveguide can be split when the fluid flow is split. This new method enables highly integrated biosensors such as lab-on-chip flow cytometers and micro-fabricated fluorescence-activated cell sorter with on-chip excitation.

  14. Cell entry by human pathogenic arenaviruses.

    Science.gov (United States)

    Rojek, Jillian M; Kunz, Stefan

    2008-04-01

    The arenaviruses Lassa virus (LASV) in Africa and Machupo (MACV), Guanarito (GTOV) and Junin viruses (JUNV) in South America cause severe haemorrhagic fevers in humans with fatality rates of 15-35%. The present review focuses on the first steps of infection with human pathogenic arenaviruses, the interaction with their cellular receptor molecules and subsequent entry into the host cell. While similarities exist in genomic organization, structure and clinical disease caused by pathogenic Old World and New World arenaviruses these pathogens use different primary receptors. The Old World arenaviruses employ alpha-dystroglycan, a cellular receptor for proteins of the extracellular matrix, and the human pathogenic New World arenaviruses use the cellular cargo receptor transferrin receptor 1. While the New World arenavirus JUNV enters cells via clathrin-dependent endocytosis, evidence occurred for clathrin-independent entry of the prototypic Old World arenavirus lymphocytic choriomeningitis virus. Upon internalization, arenaviruses are delivered to the endosome, where pH-dependent membrane fusion is mediated by the envelope glycoprotein (GP). While arenavirus GPs share characteristics with class I fusion GPs of other enveloped viruses, unusual mechanistic features of GP-mediated membrane fusion have recently been discovered for arenaviruses with important implications for viral entry.

  15. Markers of T Cell Senescence in Humans

    Directory of Open Access Journals (Sweden)

    Weili Xu

    2017-08-01

    Full Text Available Many countries are facing the aging of their population, and many more will face a similar obstacle in the near future, which could be a burden to many healthcare systems. Increased susceptibility to infections, cardiovascular and neurodegenerative disease, cancer as well as reduced efficacy of vaccination are important matters for researchers in the field of aging. As older adults show higher prevalence for a variety of diseases, this also implies higher risk of complications, including nosocomial infections, slower recovery and sequels that may reduce the autonomy and overall quality of life of older adults. The age-related effects on the immune system termed as “immunosenescence” can be exemplified by the reported hypo-responsiveness to influenza vaccination of the elderly. T cells, which belong to the adaptive arm of the immune system, have been extensively studied and the knowledge gathered enables a better understanding of how the immune system may be affected after acute/chronic infections and how this matters in the long run. In this review, we will focus on T cells and discuss the surface and molecular markers that are associated with T cell senescence. We will also look at the implications that senescent T cells could have on human health and diseases. Finally, we will discuss the benefits of having these markers for investigators and the future work that is needed to advance the field of T cell senescence markers.

  16. Human embryonic stem cells and microenvironment

    Directory of Open Access Journals (Sweden)

    Banu İskender

    2014-09-01

    Full Text Available Human embryonic stem cells (hESCs possess a great potential in the field of regenerative medicine by their virtue of pluripotent potential with indefinite proliferation capabilities. They can self renew themselves and differentiate into three embryonic germ layers. Although they are conventionally grown on mitotically inactivated mouse feeder cells, there are in vitro culture systems utilizing feeder cells of human origin in order to prevent cross-species contamination. Recently established in vitro culture systems suggested that direct interaction with feeder cells is not necessary but rather attachment to a substrate is required to ensure long-term, efficient hESC culture in vitro. This substrate is usually composed of a mixture of extracellular matrix components representing in vivo natural niche. In hESC biology, the mechanism of interaction of hESCs with extracellular matrix molecules remained insufficiently explored area of research due to their transient nature of interaction with the in vivo niche. However, an in vitro culture system established using extracellular matrix molecules may provide a safer alternative to culture systems with feeder cells while paving the way to Good Manufacturing Practice-GMP production of hESCs for therapeutic purposes. Therefore, it is essential to study the interaction of extracellular matrix molecules with hESCs in order to standardize in vitro culture systems for large-scale production of hESCs in a less labor-intensive way. This would not only provide valuable information regarding the mechanisms that control pluripotency but also serve to dissect the molecular signaling pathways of directed differentiation for prospective therapeutic applications in the future. J Clin Exp Invest 2014; 5 (3: 486-495

  17. Arecoline is cytotoxic for human endothelial cells.

    Science.gov (United States)

    Ullah, Mafaz; Cox, Stephen; Kelly, Elizabeth; Boadle, Ross; Zoellner, Hans

    2014-11-01

    Oral submucous fibrosis is a pre-malignant fibrotic condition caused by areca nut use and involves reduced mucosal vascularity. Arecoline is the principal areca nut alkaloid and is cytotoxic for epithelium and fibroblasts. Endothelial cell cycle arrest is reported on exposure to arecoline, as is cytotoxicity for endothelial-lung carcinoma hybrid cells. We here describe cytotoxicity for primary human endothelial cultures from seven separate donors. Human umbilical vein endothelial cells were exposed to increasing concentrations of arecoline and examined by: phase-contrast microscopy, haemocytometer counts, transmission electron microscopy, lactate dehydrogenase release and the methyl-thiazol-tetrazolium assay. Vacuolation and detachment of endothelium were observed at and above arecoline concentrations of 333 μg/ml or more. Ultrastructural features of cellular stress were seen after 24-h treatment with 111 μg/ml arecoline and included reduced ribosomal studding of endoplasmic reticulum, increased autophagolysosomal structures, increased vacuolation and reduced mitochondrial cristae with slight swelling. Similar changes were seen at 4 h with arecoline at 333 μg/ml or above, but with more severe mitochondrial changes including increased electron density of mitochondrial matrix and greater cristal swelling, while by 24 h, these cells were frankly necrotic. Haemocytometer counts were paralleled by both lactate dehydrogenase release and the methyl-thiazol-tetrazolium assays. Arecoline is cytotoxic via necrosis for endothelium, while biochemical assays indicate no appreciable cellular leakage before death and detachment, as well as no clear effect on mitochondrial function in viable cells. Arecoline toxicity may thus contribute to reduced vascularity in oral submucous fibrosis. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  18. Functional interactions of the AF-2 activation domain core region of the human androgen receptor with the amino-terminal domain and with the transcriptional coactivator TIF2 (transcriptional intermediary factor2)

    NARCIS (Netherlands)

    C.A. Berrevoets (Cor); P. Doesburg (Paul); K. Steketee (Karine); J. Trapman (Jan); A.O. Brinkmann (Albert)

    1998-01-01

    textabstractPrevious studies in yeast and mammalian cells showed a functional interaction between the amino-terminal domain and the carboxy-terminal, ligand-binding domain (LBD) of the human androgen receptor (AR). In the present study, the AR subdomains involved in thi

  19. Amniotic fluid-derived stem cells in regenerative medicine research.

    Science.gov (United States)

    Joo, Sunyoung; Ko, In Kap; Atala, Anthony; Yoo, James J; Lee, Sang Jin

    2012-02-01

    The stem cells isolated from amniotic fluid present an exciting possible contribution to the field of regenerative medicine and amniotic fluid-derived stem (AFS) cells have significant potential for research and therapeutic applications. AFS cells are multipotent, showing the ability to differentiate into cell types from all three embryonic germ layers. They express both embryonic and adult stem cell markers, expand extensively without feeder cells, double in 36 h, and are not tumorigenic. The AFS cells can be maintained for over 250 population doublings and preserve their telomere length and a normal karyotype. They differentiate easily into specific cell lineages and do not require human embryo tissue for their isolation, thus avoiding the current controversies associated with the use of human embryonic stem (ES) cells. The discovery of the AFS cells has been recent, and a great deal of work remains to be performed on the characterization and use of these cells. This review describes the various differentiated lineages that AFS cells can form and the future of these promising new stem cells in regenerative medicine research.

  20. Måling af stress hos mink

    DEFF Research Database (Denmark)

    Malmkvist, Jens

    2010-01-01

    temadagen præsenteres nye resultater og metoder til at vurdere stress hos mink. I en metode måles koncentrationen af nedbrydningsprodukter af hormonet cortisol i gødning fra mink. Der er en række fordele ved denne metode, frem for blodprøvetagning. Forsøg viste, at hovedparten af cortisol udskilles i fæces...... (83 %) frem for i urin (17 %), samt at omsætningen af radioaktivt mærket cortisol ikke er forskellig mellem mink med hhv. lav og høj forekomst af stereotypi. Denne viden kan bruges til bedre at forstå koblingen f.eks. unormal adfærd og stress hos mink.......Sammendrag Stress indgår som et element ved vurdering af dyrevelfærd. Endvidere kan stress mål anvendes i undersøgelser, hvor forskellige typer af avl, burmiljø og pasning af mink sammenlignes. Men hvad er stress egentlig og hvordan måles det? Er unormal adfærd koblet til stress hos mink? På...

  1. Dimensionering af fortrængningsventilation

    DEFF Research Database (Denmark)

    Nielsen, Peter Vilhelm

    2000-01-01

    Lufthastigheden og temperaturfordelingen er væsentlige elementer i bestemmelsen af den termiske komfort i rum med fortrængningsventilation. Det er derfor vigtigt at fastlægge disse størrelser i selve designproceduren. Aalborg Universitet har udviklet en forenklet metode til bestemmelse af lufthas...

  2. Effekter af slægtspleje

    DEFF Research Database (Denmark)

    Knudsen, Lajla; Egelund, Tine

    Slægtspleje er en relativt ny anbringelsesform i Danmark, og den anvendes endnu kun i beskedent omfang. Denne rapport sammenligner effekterne af slægtspleje med effekterne af traditionel familiepleje, og den har til formål at danne et vidensgrundlag for den socialpolitiske diskussion om slægtsple...

  3. Grundtvigs nationalisme i lyset af nationalismeteori

    DEFF Research Database (Denmark)

    Böss/Bøss, Michael

    2013-01-01

    af det modernistiske paradigme har gjort det muligt at diskutere Grundvigs syn på fædreland og folk på en mere nuanceret måde end tidligere. Artiklen argumenterer for, at Grundtvigs tanker om folket skal forstås som en fornyelse og demokratisering af 1700-tallets patriotisme og en geopolitisk...

  4. Deregulering af arbejdsretlige EU-direktiver

    DEFF Research Database (Denmark)

    Hasselbalch, Ole; Rønnow Bruun, Jørgen

    2013-01-01

    Europa-Kommissionen vil lette de ”reguleringsmæssige byrder” for små og mellemstore virksomheder (SMV). Langt hovedparten af de danske virksomheder er SMV´er. Konsekvenserne af initiativet kan derfor blive særdeles vidtgående på dansk grund. Artiklen drøfter, om det overhovedet er muligt i henhold...

  5. Udvikling af pille-doserings teknologi

    DEFF Research Database (Denmark)

    Brændgaard, Rikke; Churchill, Inge-Lise; Bolvig Poulsen, Søren;

    2015-01-01

    Lab. X har i samarbejde med Brønderslev Kommune og Firmaet LT Automation i et idegenereringsforløb omhandlende viden om "pillens vej", indhentet viden om udfordringer og barrierer i forbindelse med udlevering af medicin fra apoteket til den afslutningsvis indtages af borgeren. Formålet med unders...

  6. Dialogisk gruppecoaching – facilitering af ledelsesudvikling

    DEFF Research Database (Denmark)

    Alrø, Helle; Dahl, Poul Nørgård; Kloster, Per

    læring i ledergrupperne, og at skabe viden om disse udviklings- og læreprocesser. Vi har således været optaget af, hvordan dialogisk gruppecoaching kan facilitere ledelsesudvikling, og hvordan dialogisk coaching kan udvikles som coachingkoncept ift. grupper. Dialogisk gruppecoaching – facilitering af...

  7. Beskrivelse og modellering af produktionssystemer, Tulip

    DEFF Research Database (Denmark)

    Jacobsen, Peter

    1997-01-01

    produktionssystemer til brug ved optimering af drift og planlægning/udvikling af nye produktionssystemer. For at opnå et nødvendigt informationsgrundlag udføres projektet i samarbejde med udvalgte virksomheder.Rapporten er udført i samarbejde med Tulip International A/S i Vejle, hvor den del, der omfatter...

  8. Hvad forklarer forskellige typer af frivilligt engagement?

    DEFF Research Database (Denmark)

    Henriksen, Lars Skov; Rosdahl, David

    2008-01-01

    Artiklen undersøger empirisk gennem multivariate analyser betydningen af forskellige former for personlige og sociale ressourcer for tre forskellige typer af formelt frivilligt arbejde: aktivitetsorienteret frivilligt arbejde, velfærdsorienteret frivilligt arbejde samt politisk og (lokal)samfunds......)samfundsorienteret frivilligt engagement....

  9. Kritikken af new public management er forfejlet

    DEFF Research Database (Denmark)

    Klausen, Kurt Klaudi

    2016-01-01

    kronikken adresserer og korrigerer den opfattelse at de nyliberalistiske reformer under betegnelsen NPM ikke har bidraget positivt til moderniseringen af den offentlige sektor.......kronikken adresserer og korrigerer den opfattelse at de nyliberalistiske reformer under betegnelsen NPM ikke har bidraget positivt til moderniseringen af den offentlige sektor....

  10. Grønland stiger af havet

    DEFF Research Database (Denmark)

    Laursen, Steen; Khan, Shfaqat Abbas

    2012-01-01

    En af de varme sommernyheder er, at Grønlands bjergtinder stiger mod himmelen i takt med, at indlandsisen tynder ud. Det viser dansk forskning, som bliver bakket op af amerikanske målinger fra Nasa. Newton har besøgt Nasa på Grønland og interviewet den danske forsker bag de nye resultater....

  11. Sundhedspleje - Studier af en normaliserende praksis

    DEFF Research Database (Denmark)

    Pedersen, Oline

    2013-01-01

    Phd.-afhandling Afhandlingen er en analyse af sundhedsplejens praksis. Dener udført på baggrund af etnografisk feltarbejde i sundhedsplejen (suppleret med interviews). Afhandlingen har tre undersøgelsesspørgsmål: Hvordan formidles normalitet i sundhedsplejens praksis? Hvilke former for normalitet...

  12. Økonomisk Vurdering af Arbejdsulykker

    DEFF Research Database (Denmark)

    Rikhardsson, Pall M.; Mogensen, Birgitte; Ravn, Birgitte

    Formålet med rapporten er at præsentere en metode og en brugsanvisning, der kan anvendes til registrering og kortlægning af økonomiske omkostninger/effekter ved arbejdsulykker (ulykkesomkostninger) samt en guideline til brug ved konkretisering af de mere uhåndgribelige afledte effekter, som disse...

  13. Prioriteringer af efterretninger i kampen mod terror

    DEFF Research Database (Denmark)

    Nielsen, Thomas Galasz

    2015-01-01

    Efterretningstjenesterne indsamler stadig flere informationer i forsøget på at opdage og imødegå terrorister. Men mængden af informationer gør det ikke nødvendigvis lettere at forudsige terrorhandlinger. Efterretningstjenesterne bør derfor prioritere bearbejdning af oplysningerne højere, end de g...

  14. Udvikling af intelligent legepraksis i trampoliner

    DEFF Research Database (Denmark)

    Elbæk, Lars; Karoff, Helle Skovbjerg; Rytz Alison, Sigrid

    2012-01-01

    digitale aktiviteter. Det sikkerhedsmæssige er afgørende at medtænke, når der designes et digitalt add-on til en trampolin. Hvordan kan det fremmes uden at legen hurtigt bliver kedelig? Dette var en af de store udfordringer i udviklingen af den intelligente trampolin, som der redegøres for i artiklen...

  15. Tema 1: Programmering af robotenheder i grundskolen

    Directory of Open Access Journals (Sweden)

    Stine Ejsing-Duun

    2016-01-01

    Full Text Available I denne artikel undersøger vi potentialerne for at lære matematik gennem programmering af robotter. Vi foreslår en model med tre indbyrdes afhængige læringspotentialer, som programmering fordrer. Det omfatter elevens evne til at; (1 tænke i algoritmer, (2 producere viden og artefakter gennem brug af matematik og (3 foretage abstraktion og indkapsling. Programmering er blevet en del af grundskolens læringsmål i flere lande. I Danmark er programmering et læringsmål i forenklede fælles mål for Fysik/Kemi og desuden en del af den nationale undervisningsvejledning for matematik. En analyse af potentialerne i at anvende programmering i forbindelse med disse fag er derfor væsentlig. I denne artikel fokuserer vi på samspillet mellem matematik og programmering gennem litteraturstudier og analyse af empiriske situationer fra undervisning i programmering af LEGO Mindstorms. Teoretisk anvendes den instrumentelle tilgang til teknologi i matematikundervisning. Analysen viser en række måder, hvorpå didaktisk opmærksomhed på epistemisk forhandling kan understøtte læring af matematik gennem programmering.

  16. Gyldigheden af antagelsen om Gaussisk Turbulens

    DEFF Research Database (Denmark)

    Nielsen, Morten; Hansen, Kurt S.; Pedersen, Bo Juul

    2000-01-01

    Vindmøller designes til at modstå virkningen af den turbulente vind, og i den forbindelse plejer men at antage at hastighedens sansynlighed er normalfordelt. Det synes, på baggrund af et stort antal målinger fra flere lokaliteter, at være en rimelig antagelse i fladt homogent terræn, men der find...

  17. Tre generationer af Flow Injection Analysis

    DEFF Research Database (Denmark)

    Hansen, Elo Harald; Chomchoei, Roongrat; Long, Xiangbao

    2004-01-01

    Siden introduktionen af FIA har en række videreudviklinger resulteret i Sequential Injection Analysis (SIA) og senest i Lab-on-Valve (LOV)-metodikken. Her beskrives disse, og der gives eksempler på, hvordan metoderne kan bruges til at bestemme sporstofkoncentrationer af metaller i komplekse...

  18. Cyberwarfares udfordringer af begrebet kritisk infrastruktur

    DEFF Research Database (Denmark)

    Lauta, Kristian Cedervall; Hoffmann, Rune; Struwe, Lars Bangert

    I dag er der en stigende bekymring for cyberwarfare eller cyberkriminalitet. Det skyldes, at det moderne samfund er komplekst og sårbart. Samfundet baserer sig på udbuddet af stadig flere funktioner, som bliver tiltagende avancerede og internt afhængige af hinanden. Systemet bliver herved så avan...

  19. Manual reduktion af pattegrisedødelighed

    DEFF Research Database (Denmark)

    Bonde, Marianne; Serup, Tove

    svært at opretholde en målrettet indsats. Aarhus Universitet, Det Jordbrugsvidenskabelige Fakultet, Foulum har siden 2007 arbejdet på forskningsprojektet Reduceret pattegrisedødelighed og fravænningsdiarre. Et af produkterne fra dette projekt er denne manual for reduktion af pattegrisedødelighed...

  20. Organisering af projektudvikling - KulturarvNord

    DEFF Research Database (Denmark)

    Mark, Stine

    Organisering af projektudvikling - KulturarvNord er udarbejdet af forskningscenteret INCEVIDA, Aalborg Universitet 2012, og gennemført i et samarbejde mellem INCEVIDA og museumsnetværket KulturarvNord. I rapporten tages der udgangspunkt i empirisk materiale indhentet via interviews med nøgleperso...

  1. Udvikling af professionelle kompetencer i klinisk praksis

    DEFF Research Database (Denmark)

    Thrysøe, Lars

    2013-01-01

    kulturelt indlejret, hvor læring er en kontinuerlig proces og tæt knyttet til den kontekst, hvori aktiviteten foregår. Med afsæt i Berger og Luckmanns socialiseringsteori argumenteres for, at deltagelse er særlig central ved udvikling af professionelle kompetencer i klinisk praksis, og hvor også (erhvervs...

  2. Digitale leverancer ved aflevering af byggeri

    DEFF Research Database (Denmark)

    Bertelsen, Niels Haldor; Sørensen, Nils Lykke; Shaukat, Fatima

    Udviklingen af det digitale byggeri i Danmark har haft meget fokus på klassifikation, fælles standarder og digitale værktøjer, som skal understøtte en centralt styret bygningsmodel. Der er ved at ske et paradigmeskift, hvor den praktiske anvendelse af IKT i de enkelte faggrupper er mere i centrum...

  3. Forført af hjernen?

    DEFF Research Database (Denmark)

    Lieberoth, Andreas

    2013-01-01

    Blandingen af offentlig interesse, gode mediehistorier og smarte sælgere har ført til sejlivede neuromyter, der ikke bare distraherer almindelige mennesker, men også fører til udbredte misforståelser blandt professionelle som lærere og plejepersonale. I bedste fald leder neuromyter til spild af t...

  4. Måling af virksomheders administrative byrder

    DEFF Research Database (Denmark)

    Langsted, Lars Bo

    2004-01-01

    Selskabsstyrelsen har offentliggjort rapporten: "Rapport om måling af virksomhedernes administrative byrder ved årsregnskabsloven og rapport om revisionshonorar for B-virksomheder."......Selskabsstyrelsen har offentliggjort rapporten: "Rapport om måling af virksomhedernes administrative byrder ved årsregnskabsloven og rapport om revisionshonorar for B-virksomheder."...

  5. High-resolution micropatterned Teflon AF substrates for biocompatible nanofluidic devices.

    Science.gov (United States)

    Czolkos, Ilja; Hakonen, Bodil; Orwar, Owe; Jesorka, Aldo

    2012-02-14

    We describe a general photolithography-based process for the microfabrication of surface-supported Teflon AF structures. Teflon AF patterns primarily benefit from superior optical properties such as very low autofluorescence and a low refractive index. The process ensures that the Teflon AF patterns remain strongly hydrophobic in order to allow rapid lipid monolayer spreading and generates a characteristic edge morphology which assists directed cell growth along the structured surfaces. We provide application examples, demonstrating the well-controlled mixing of lipid films on Teflon AF structures and showing how the patterned surfaces can be used as biocompatible growth-directing substrates for cell culture. Chinese hamster ovary (CHO) cells develop in a guided fashion along the sides of the microstructures, selectively avoiding to grow over the patterned areas.

  6. Identification of a candidate stem cell in human gallbladder

    Directory of Open Access Journals (Sweden)

    Rohan Manohar

    2015-05-01

    In conclusion, we have isolated a distinct clonogenic population of epithelial cells from primary human fetal gallbladder with stem cell characteristics and found it to be unique compared to IHBD cells.

  7. Cell cycle regulation by glucosamine in human pulmonary epithelial cells.

    Science.gov (United States)

    Chuang, Kun-Han; Lu, Chih-Shen; Kou, Yu Ru; Wu, Yuh-Lin

    2013-04-01

    Airway epithelial cells play an important role against intruding pathogens. Glucosamine, a commonly used supplemental compound, has recently begun to be regarded as a potential anti-inflammatory molecule. This study aimed to uncover how glucosamine impacts on cellular proliferation in human alveolar epithelial cells (A549) and bronchial epithelial cells (HBECs). With trypan blue-exclusion assay, we observed that glucosamine (10, 20, 50 mM) caused a decrease in cell number at 24 and 48 h; with a flow cytometric analysis, we also noted an enhanced cell accumulation within the G(0)/G(1) phase at 24 h and induction of late apoptosis at 24 and 48 h by glucosamine (10, 20, 50 mM) in A549 cells and HBECs. Examination of phosphorylation in retinoblastoma (Rb) protein, we found an inhibitory effect by glucosamine at 20 and 50 mM. Glucosamine at 50 mM was demonstrated to elevate both the mRNA and protein expression of p53 and heme oxygenase-1 (HO-1), but also caused a reduction in p21 protein expression. In addition, glucosamine attenuated p21 protein stability via the proteasomal proteolytic pathway, as well as inducing p21 nuclear accumulation. Altogether, our results suggest that a high dose of glucosamine may inhibit cell proliferation through apoptosis and disturb cell cycle progression with a halt at G(0)/G(1) phase, and that this occurs, at least in part, by a reduction in Rb phosphorylation together with modulation of p21, p53 and HO-1 expression, and nuclear p21 accumulation.

  8. Review: Brug af data i skoleledelse

    DEFF Research Database (Denmark)

    Hornskov, Søren; Bjerg, Helle; Nielsen, Laura Høvsgaard

    2015-01-01

    Reviewet skaber overblik over den internationale litteratur om brug af data i skoleledelse. Formålet er at finde frem til forskning, der udpeger og beskriver de faktorer, som henholdsvis hæmmer og fremmer brugen af data i grundskolen. Arbejdsgruppen arbejdede ud fra en interesse i at finde frem til...... forskning, som havde noget at sige om betingelser for produktiv brug af data i praksis med relevans i dansk sammenhæng. Review-spørgsmål: hvilke egenskaber ved ledelse og organisation, som er relevante i forhold til opbygning af professionel kapacitet i danske skoler, henholdsvis hæmmer og fremmer brugen af...... data i grundskolen?...

  9. Miljøvurdering af isoleringsmetoder

    DEFF Research Database (Denmark)

    Krogh, H.; Rasmussen, J.O.; Nielsen, P.A.

    Når man skal gennemføre miljørigtigt byggeri er det nødvendigt at have adgang til miljødata for materialerne der knytter sig til produktet gennem hele dets livsforløb. I denne rapport præsenteres kvantitative miljødata for fremstilling af isoleringsprodukter. Det drejer sig om løsfyldsproduklter ...... cellulosefibre, ruller og plader af hør samt produkter af perlite. Rapporten viser også, hvordan disse data kan benyttes til at beregne miljøbelastninger for hele bygningsdele. Desuden gives en kvantitativ vurdering af produkternes miljøpåvirkninger af indeklimaet og ved bortskaffelse...

  10. Røntgenundersøgelse af lungerne

    DEFF Research Database (Denmark)

    Debess, Jeanne Elisabeth; Vejle-Sørensen, Jens Kristian; Johnsen, Karen Kirstine

    2014-01-01

    var 306.662 (5,3%) udført på kvinder (Statens serum institut). Stråledosen på 0,1 mSv (Strålingsguiden ioniserende stråling 2013) til den enkelte patient er lille, men det store antal undersøgelser medfører at den kollektive stråledosis er af væsentlig betydning. En mulig nedsættelse af dosis til den...... baggrund af fokus på kollimeringens betydning for dosis til patienterne. Delmål I: At vurdere antallet af røntgenbilleder af thorax med henholdsvis korrekt kollinering. Delmål II: At undersøge dosis til risikoorganerne mammae, thyroidea og ventrikel ved kollimering ud over den pr. protokol fastsatte...

  11. Human embryonic stem cells and patent protection

    Directory of Open Access Journals (Sweden)

    Radovanović Sanja M.

    2015-01-01

    Full Text Available Given the importance of biotechnological research in modern diagnostics and therapeutics, on the one hand, and stimulative function of a patent, on the other hand, this work deals with the question of the possibility of pa-tent protection of human embryonic stem cells. Taking into account that this is a biotechnological invention, the key question that this paper highlights is the interpretation of the provisions of their patentability. Namely, thanks to the advanced methods of isolation, purification and preparation for implementation, modern patent systems do not exclude a priori living organisms from patent protection. Therefore, the analysis of representative administrative decisions or court rulings sought to define the criteria that would be applied in order to give patent protection to a certain biotechnological invention (stem cells while others do not.

  12. Generation of human induced pluripotent stem cells from dermal fibroblasts

    OpenAIRE

    2008-01-01

    The generation of patient-specific pluripotent stem cells has the potential to accelerate the implementation of stem cells for clinical treatment of degenerative diseases. Technologies including somatic cell nuclear transfer and cell fusion might generate such cells but are hindered by issues that might prevent them from being used clinically. Here, we describe methods to use dermal fibroblasts easily obtained from an individual human to generate human induced pluripotent stem (iPS) cells by ...

  13. Restriction of human adenovirus replication in Chinese hamster cell lines and their hybrids with human cells.

    Science.gov (United States)

    Radna, R L; Foellmer, B; Feldman, L A; Francke, U; Ozer, H L

    1987-11-01

    We have found that the replication of human adenovirus (Ad2) is restricted in multiple Chinese hamster cell lines including CHO and V79. The major site of restriction involves differential accumulation of late viral proteins as demonstrated by immunofluorescence assay and polyacrylamide gel electrophoresis with and without prior immunoprecipitation. Synthesis of fiber and penton base are markedly reduced, whereas others, such as the 100K polypeptide, are synthesized efficiently. This pattern of restriction is similar to that previously reported for Ad2 infection of several monkey cell lines; however, the restriction is more marked in the Chinese hamster cell lines. The restriction is most likely due to a deficient cellular function since stable cell hybrids between V79 or CHO and human cells are permissive for virus replication. By analysis of a series of hybrids with reduced numbers of human chromosomes, fiber synthesis was correlated with the presence of the short arm of human chromosome 3. More hybrids showed restoration of fiber synthesis than production of progeny virus, suggesting that more than one unlinked function is required for the latter.

  14. Gymnasterkoreaynes A-F, cytotoxic polyacetylenes from Gymnaster koraiensis.

    Science.gov (United States)

    Jung, Hyun-Ju; Min, Byung-Sun; Park, Jin-Young; Kim, Young-Ho; Lee, Hyeong-Kyu; Bae, Ki-Hwan

    2002-06-01

    Six new polyacetylenes, gymnasterkoreaynes A-F (1-6), were isolated from the roots of Gymnaster koraiensis, together with 2,9,16-heptadecatrien-4,6-diyn-8-ol (7) and 1,9,16-heptadecatriene-4,6-diyn-3,8-diol (8), by bioassay-guided fractionation using the L1210 tumor cell line as a model for cytotoxicity. The structures of compounds 1-6 were established spectroscopically, which included 2D NMR experiments. Gymnasterkoreaynes A-F (1-6) are linear diacetylenes and are structurally related to falcarinol, panaxynol, panaxydiol, and panaxytriol. Of the compounds isolated, gymnasterkoreaynes B (2), C (3), F (6), and 1,9,16-heptadecatrien-4,6-diyn-3,8-diol (8) exhibited significant cytotoxicity against L1210 tumor cells with ED(50) values of 0.12-3.3 microg/mL.

  15. Phenotypic changes of human cells in human-rat liver during partial hepatectomy-induced regeneration

    Institute of Scientific and Technical Information of China (English)

    Yan Sun; Dong Xiao; Hong-An Li; Jin-Fang Jiang; Qing Li; Ruo-Shuang Zhang; Xi-Gu Chen

    2009-01-01

    AIM: To examine the human hepatic parenchymal and stromal components in rat liver and the phenotypic changes of human cells in liver of human-rat chimera (HRC) generated by in utero transplantation of human cells during partial hepatectomy (PHx)-induced liver regeneration. METHODS: Human hepatic parenchymal and stromal components and phenotypic changes of human cells during liver regeneration were examined by flow cytometry, in situ hybridization and immunohistochemistry. RESULTS: ISH analysis demonstrated human Alupositive cells in hepatic parenchyma and stroma of recipient liver. Functional human hepatocytes generated in this model potentially constituted human hepatic functional units with the presence of donor-derived human endothelial and biliary duct cells in host liver. Alpha fetoprotein (AFP)+, CD34+ and CD45+ cells were observed in the chimeric liver on day 10 after PHxinduced liver regeneration and then disappeared in PHx group, but not in non-PHx group, suggesting that dynamic phenotypic changes of human cells expressing AFP, CD34 and CD45 cells may occur during the chimeric liver regeneration. Additionally, immunostaining for human proliferating cell nuclear antigen (PCNA) showed that the number of PCNA-positive cells in the chimeric liver of PHx group was markedly increased, as compared to that of control group, indicating that donor-derived human cells are actively proliferated during PHx-induced regeneration of HRC liver.

  16. HIF induces human embryonic stem cell markers in cancer cells.

    Science.gov (United States)

    Mathieu, Julie; Zhang, Zhan; Zhou, Wenyu; Wang, Amy J; Heddleston, John M; Pinna, Claudia M A; Hubaud, Alexis; Stadler, Bradford; Choi, Michael; Bar, Merav; Tewari, Muneesh; Liu, Alvin; Vessella, Robert; Rostomily, Robert; Born, Donald; Horwitz, Marshall; Ware, Carol; Blau, C Anthony; Cleary, Michele A; Rich, Jeremy N; Ruohola-Baker, Hannele

    2011-07-01

    Low oxygen levels have been shown to promote self-renewal in many stem cells. In tumors, hypoxia is associated with aggressive disease course and poor clinical outcomes. Furthermore, many aggressive tumors have been shown to display gene expression signatures characteristic of human embryonic stem cells (hESC). We now tested whether hypoxia might be responsible for the hESC signature observed in aggressive tumors. We show that hypoxia, through hypoxia-inducible factor (HIF), can induce an hESC-like transcriptional program, including the induced pluripotent stem cell (iPSC) inducers, OCT4, NANOG, SOX2, KLF4, cMYC, and microRNA-302 in 11 cancer cell lines (from prostate, brain, kidney, cervix, lung, colon, liver, and breast tumors). Furthermore, nondegradable forms of HIFα, combined with the traditional iPSC inducers, are highly efficient in generating A549 iPSC-like colonies that have high tumorigenic capacity. To test potential correlation between iPSC inducers and HIF expression in primary tumors, we analyzed primary prostate tumors and found a significant correlation between NANOG-, OCT4-, and HIF1α-positive regions. Furthermore, NANOG and OCT4 expressions positively correlated with increased prostate tumor Gleason score. In primary glioma-derived CD133 negative cells, hypoxia was able to induce neurospheres and hESC markers. Together, these findings suggest that HIF targets may act as key inducers of a dynamic state of stemness in pathologic conditions.

  17. Danish strategy for developing SOFC fuel cells 2010-2020; Dansk strategi for udvikling af SOFC-braendselsceller 2010-2020

    Energy Technology Data Exchange (ETDEWEB)

    Smith, A.; Linderoth, S. (Technical Univ. of Denmark, Risoe National Lab. for Sustainable Energy, Roskilde (Denmark)); Themsen, J. (Dantherm Power A/S, Hobro (Denmark)); Richter, A.B.; Hansen, Hakon J.; Holm-Larsen, H.; Andersen, Claus V. (Topsoe Fuel Cell A/S, Lyngby (Denmark))

    2010-09-15

    SOFC fuel cells are a promising technology for efficient production of electricity. The technology is expected to contribute very significantly to climate and environmental goals, security of supply of energy, increased employment and exports. Denmark has a very strong technology position within the SOFC area based on years of cooperation between Topsoe Fuel Cell and Risoe DTU as well as a later developed relationship with Dantherm Power. The Danish players have received more than DKK 250 million in public funding. With this support and the players' own funding (approx. two times higher than public support), the players managed over the past 10 years to bring development of SOFC technology significantly closer to the market. This strategy has been prepared by the Danish players in the field and gives a picture of the required overall development needs in the coming years. Technology development has reached the stage where in the coming years demonstrations on a larger scale have to be launched. The focus is basically on three valuable market segments: micro power and heat, auxiliary power for mobile applications, and decentralized power and CHP, which all have a great potential for market penetration internationally. When the market is established in one or more of these segments, other business opportunities must be explored for other applications based on the established technological platform. The road to commercialization is a long process that begins with demonstrations and later introduction of commercial products. The primary actors are currently the largest companies and institutions but as the number of produced fuel cell units increases, job creation with subcontractors and other directly related businesses and research centres will increase. A conservative estimate of market size worldwide is 2-4 GW of installed capacity per. year representing an annual turnover of 15-30 billion DKK and 10.000-20.000 more jobs. The export ratio is expected to exceed

  18. Derivation of human embryonic stem cells in defined conditions.

    Science.gov (United States)

    Ludwig, Tenneille E; Levenstein, Mark E; Jones, Jeffrey M; Berggren, W Travis; Mitchen, Erika R; Frane, Jennifer L; Crandall, Leann J; Daigh, Christine A; Conard, Kevin R; Piekarczyk, Marian S; Llanas, Rachel A; Thomson, James A

    2006-02-01

    We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.

  19. Therapeutic outcomes of transplantation of amniotic fluid-derived stem cells in experimental ischemic stroke

    Directory of Open Access Journals (Sweden)

    Naoki eTajiri

    2014-08-01

    Full Text Available Accumulating preclinical evidence suggests the use of amnion as a source of stem cells for investigations of basic science concepts related to developmental cell biology, but also for stem cells’ therapeutic applications in treating human disorders. We previously reported isolation of viable rat amniotic fluid-derived stem (AFS cells. Subsequently, we recently reported the therapeutic benefits of intravenous transplantation of AFS cells in a rodent model of ischemic stroke. Parallel lines of investiagtions have provided safety and efficacy of stem cell therapy for treating stroke and other neurological disorders. This review article highlights characterization of AFS cells’ phenotype and their transplant-mediated functional effects, the need for investigations of mechanisms underlying AFS cells’ therapeutic benefits and discusses lab-to-clinic translational gating items in an effort to optimize the clinical application of cell transplantation for stroke.

  20. Optimering af behandlingseffekten i akvakultur. Minimering af forbrug og udledning af hjælpestoffer

    DEFF Research Database (Denmark)

    Sortkjær, Ole; Henriksen, Niels Henrik; Demuth Heinecke, Rasmus;

    er 1/5 af, hvad der normalt anvendes. Brintoverilte omsættes hurtigt, så det er nødvendigt at supplere med brintoverilte under behandlingen i damme. Forsøg med biofilter viser, at formalin omsættes hurtigere, hvis det i forvejen har været eksponeret med formalin, og det skader ikke den mikrobielle...... behandlingstiden samtidig bliver forlænget, sådan som toksicitetstestene viser. For at bekæmpe fiskedræberen skal der behandles flere gange med nogle dages mellemrum, når dambruget er hårdt inficeret. I recirkulerende anlæg med biofilter er det nødvendigt at supplere med formalin for at holde koncentrationen under...