Hyperpolarized 3He MRI and 81mKr SPECT in chronic obstructive pulmonary disease

DEFF Research Database (Denmark)

Stavngaard, Trine; Søgaard, Lise; Mortensen, J

2005-01-01

visual defect score (r=0.80, pemphysema (pulmonary function test and HRCT). The defect scores were largest on 81mKr SPECT (the score on HP 3He MRI...... was to compare ventilation imaging methods in 26 patients with chronic obstructive pulmonary disease (COPD) and nine lung healthy volunteers. METHODS: HP 3He MRI, 81mKr single-photon emission computed tomography (SPECT), high-resolution computed tomography (HRCT) and pulmonary function tests were performed....... The three scans were scored visually as percentage of non-ventilated/diseased lung, and a computer-based objective measure of the ventilated volume in HP 3He MRI and 81mKr SPECT and an emphysema index in HRCT were calculated. RESULTS: We found a good correlation between HP 3He MRI and 81mKr SPECT for both...

Cytokine and surface receptor diversity of NK cells in resistant C3H/HeN and susceptible BALB/c mice with chronic Pseudomonas aeruginosa lung infection

DEFF Research Database (Denmark)

Calum, Henrik; Moser, Claus; Jensen, Peter Østrup

2003-01-01

The purpose of the present study was to investigate whether NK cells from resistant C3H/HeN mice and susceptible BALB/c mice showed different release of cytokines and expression of surface molecules during chronic P. aeruginosa lung infection using alginate-embedded P. aeruginosa mimicking...... expression of the LFA-1 and Fc receptors on NK cells. At day 2, IFN-gamma levels increased in C3H/HeN mice but decreased in BALB/c mice. The GM-CSF levels increased only in the C3H/HeN mice at day 1 and 2. Surface expression of LFA-1 on the NK cells was higher in C3H/HeN mice at day 1 and 2. In contrast...

Inhibition of fibroblast growth factor receptor 3-dependent lung adenocarcinoma with a human monoclonal antibody

Directory of Open Access Journals (Sweden)

Yongjun Yin

2016-05-01

Full Text Available Activating mutations in fibroblast growth factor receptor 3 (FGFR3 have been identified in multiple types of human cancer and in congenital birth defects. In human lung cancer, fibroblast growth factor 9 (FGF9, a high-affinity ligand for FGFR3, is overexpressed in 10% of primary resected non-small cell lung cancer (NSCLC specimens. Furthermore, in a mouse model where FGF9 can be induced in lung epithelial cells, epithelial proliferation and ensuing tumorigenesis is dependent on FGFR3. To develop new customized therapies for cancers that are dependent on FGFR3 activation, we have used this mouse model to evaluate a human monoclonal antibody (D11 with specificity for the extracellular ligand-binding domain of FGFR3, that recognizes both human and mouse forms of the receptor. Here, we show that D11 effectively inhibits signaling through FGFR3 in vitro, inhibits the growth of FGFR3-dependent FGF9-induced lung adenocarcinoma in mice, and reduces tumor-associated morbidity. Given the potency of FGF9 in this mouse model and the absolute requirement for signaling through FGFR3, this study validates the D11 antibody as a potentially useful and effective reagent for treating human cancers or other pathologies that are dependent on activation of FGFR3.

Infrared spectra of 4HeH+, 4HeD+, 3HeH+, and 3HeD+

International Nuclear Information System (INIS)

Crofton, M.W.; Altman, R.S.; Haese, N.N.; Oka, T.

1989-01-01

Isotopic species of the HeH + molecular ion provide an excellent testing ground for studying isotopic dependence of vibration--rotation constants because of the small masses of He and H isotopes. We have observed infrared spectra of the hot band v=2 left-arrow 1 of HeH + and fundamental bands of isotopic species HeD + , 3 HeH + , and 3 HeD + , and obtained the Dunham coefficients Y kl , and the isotopically independent parameters U kl , Δ He kl , and Δ H kl

Superfluid 3He dynamcs in 3He - 4He solutions

International Nuclear Information System (INIS)

Mejerovich, A.Eh.

1984-01-01

The dynamics of a 3 He- 4 He superfluid solution with two condensates ( 3 He and 4 He) is investigated. Despite the fact that the hydrodynamics of the system is a three-velocity one (two superfluid and one normal velocity), all the thermo- and hydrodynamic functions are determined by the value of only a single linear combination of the velocities. 0n the basis of an analogy between a moving solution and a BCS system with coupling with a non-zero momentum, the dependence of the thermodynamic quantities on the velocities and critical velocities can easily be calculated for both homogeneous and inhomogeneous phases of the solution. In a magnetic field the temperature oscillations (analogue of second sound for a superfluid solution) are accompanied by oscillations of the magnetic moment. The velocity and damping of the spin-temperature waves are determined. The orienting action of a current on the inhomogeneous phases of the solution is discussed. It is shown that the energy and size of the vortexes in a superfluid solution are, due to drag effects, oscillating functions of the effective mass of the 3 He quasirartictes (pressure). At a pressure of the order of 10 atm a first order transition should take place in the vortex line which is accompanied by an abrupt change of the circulations of superfluid velocity of 3 He for a fixed circulation of the 4 He velocity

NMR and superfluidity of 3He in 3He-4He solutions

International Nuclear Information System (INIS)

Ivanova, K.D.; Mejerovich, A.Eh.

1986-01-01

Two possibilities of determining the superfluid transition temperature for 3 He in a 3 He- 4 He solution by the NMR technique are discussed. One of the methods consists in measuring the spin diffusion coefficient in weak magnetic fields at ultralow temperatures, and the other in measuring the ratio of the spin diffusion coefficient to the spin wave absorption coefficient at not very low temperatures. The transition temperature is estimated on the basis of the available experimental data. The effect of the superfluid transition in a system of 3 He quasiparticles on the propagation of transverse spin waves and longitudinal spin-sound oscillations in 3 He- 4 He solutions is studied. It is shown that there is a range of weak magnetic field intensities restricted from both sides in which the propagation of weakly damped spin-sound waves is possible

The sup(40,44,48)Ca(3He, 3He) and (3He, 4He) reactions at 50 MeV

International Nuclear Information System (INIS)

Griffiths, R.J.; Duggan, F.; Clarke, N.M.

1981-01-01

Measurements have been made of the ( 3 He, 3 He) and ( 3 He, 4 He) reactions on the isotopes of calcium 40 Ca, 44 Ca and 48 Ca at an energy of 50.4 MeV. The results have been analysed with conventional optical potentials and DWBA. The adequacy of the former and inadequacy of the latter for reactions with composite particles is discussed. (author)

Combined He3 cryostats and He3-He4 dilution refrigerators

International Nuclear Information System (INIS)

Kovac, L.; Balla, J.

1974-07-01

A modular set of equipment was designed, which consists of a 4 He Dewar vessel with a very low evaporation rate, having a pumped 4 He bath in which either a 3 He cryostat or a dilution refrigerator within removable inserts can be placed. Any of them can be simply and rapidly connected to the versatile Dewar, auxiliary 4 He-, vacuum; and 3 He- 4 He systems. Two such sets have already been completed and can be used at temperatures from 1.5K to 0.05K for thermodynamic and neutron diffraction measurements. The performance of all inserts was stable and reliable, differences between the cryostats and runs were small - nearly all within the accuracy of temperature determination. The construction of a lot of parts is identical, allowing rapid manufacture. Assembling and repairs are simple, all parts are easily accessible. (K.A.)

X-ray-induced thinning of 3He and 3He/4He mixture films

International Nuclear Information System (INIS)

Penanen, Konstantin; Fukuto, Masafumi; Silvera, Isaac F.; Pershan, Peter

2000-01-01

Films of isotopic mixtures of helium have been studied using x-ray specular reflectivity techniques. In contrast with superfluid 4 He films, x-ray exposure causes a reduction in the thickness of 4 He films above the superfluid transition as well as films of pure 3 He and 3 He/ 4 He mixtures. One proposed model that could account for this effect is a charging model, in which thinning is caused by electrostatic pressure of free charges that accumulate on the helium surface. Unfortunately, this model is not fully consistent with all of the experimental observations. A localized heating model, in which indirect heating of the film causes it to thin would explain the data if there were dissipative film flow in the 3 He/ 4 He mixtures at temperatures where the bulk is superfluid. We argue that various published experimental results suggest such an effect. In this model, film thinning data for dilute 3 He/ 4 He films indicates dissipation that is linear in 3 He content of the film over two orders of magnitude

Heterogeneous disease progression and treatment response in a C3HeB/FeJ mouse model of tuberculosis

Directory of Open Access Journals (Sweden)

Jean-Philippe Lanoix

2015-06-01

Full Text Available Mice are the most commonly used species for non-clinical evaluations of drug efficacy against tuberculosis (TB. Unlike commonly used strains, C3HeB/FeJ mice develop caseous necrosis in the lung, which might alter the representation of drug efficacy in a way that is more like human TB. Because the development of such pathology requires time, we investigated the effect of infection incubation period on the activity of six drugs in C3HeB/FeJ and BALB/c mice. Mice were aerosol infected and held for 6, 10 or 14 weeks before receiving therapy with rifampin (RIF, rifapentine (RPT, pyrazinamide (PZA, linezolid (LZD, sutezolid (PNU or metronidazole (MTZ for 4-8 weeks. Outcomes included pathological assessments, pH measurements of liquefied caseum and assessment of colony-forming unit (CFU counts from lung cultures. Remarkable heterogeneity in the timing and extent of disease progression was observed in C3HeB/FeJ mice, largely independent of incubation period. Likewise, drug efficacy in C3HeB/FeJ mice was not affected by incubation period. However, for PZA, LZD and PNU, dichotomous treatment effects correlating with the presence or absence of large caseous lesions were observed. In the case of PZA, its poor activity in the subset of C3HeB/FeJ mice with large caseous lesions might be explained by the pH of 7.36±0.09 measured in liquefied caseum. This study highlights the potential value of C3HeB/FeJ mice for non-clinical efficacy testing, especially for investigating the interaction of lesion pathology and drug effect. Careful use of this model could enhance the bridging of non-clinical results with clinical outcomes.

Specific heat of amorphous 3He films and confined liquid 3He

International Nuclear Information System (INIS)

Golov, A.; Pobell, F.

1995-01-01

We have measured the heat capacities of 3 He films and liquid 3 He in porous Vycor glass at 10 to 600 mK. With increasing the film thickness front 1 to 3 atomic layers , the specific heat evolves gradually from that typical to solid to that of liquid 3 He. At about 2 atomic layers, however, its low-temperature part is nearly temperature-independent; we interpret this as a result of gradual freezing of spins in an amorphous solid 3 He film with decreasing the temperature. The contribution of liquid 3 He in the center of the Vycor pores can be described as the specific heat of bulk liquid 3 He at corresponding pressures in the range 0 to 28 bar. The thickness of amorphous solid on the pore walls increases with external pressure roughly linearly. Preplating the walls with 4 He allows to determine the positions of 3 He atoms contributing to the surface specific heat at 10 to 50 mK. In addition, the contribution from the specific heat of 3 He- 4 He mixing at 100 to 600 mK is discussed as a function of pressure and amount of 4 He

Attractive well of He--He from 3He--4He differential elastic scattering measurements

International Nuclear Information System (INIS)

Burgmans, A.L.J.; Farrar, J.M.; Lee, Y.T.

1976-01-01

The elastic differential cross section for 3 He-- 4 He was measured at a relative collision energy of 0.799x10 -14 erg, approximately five times the well depth. The data are fitted to a multiparameter potential form with epsilon/k=10.57 degreeK and r/subm/=2.97 A. Comparisons with recent experimental and theoretical helium potentials are made. No evidence for a significant isotope effect in the 3 He-- 4 He and 4 He-- 4 He interactomic potentials is found in this work

Superfluidity of a dilute 3He-4He solution

International Nuclear Information System (INIS)

Soda, Toshio

1993-01-01

The interaction between two 3 He atoms is calculated by taking into account the backflow effect of 3 He by the 4 He in the 3 He- 4 He mixture. The effect contributes solely to the P wave part of the interaction. The repulsive S wave part of the contact interaction contributes to the exchange interaction between the 3 He atoms, while the direct one phonon exchange interaction contributes both to the S and P wave attractive interactions. The overall contribution to the attractive interaction is dominated by the P wave part and the superfluidity in the P wave is more predominant than in the S wave for the 5 % dilute 3 He- 4 He solution, and vice versa for the 1.3 % solution. (author)

3He flow in dilute 3He-4He mixtures at temperatures between 10 and 150 mK

International Nuclear Information System (INIS)

Castelijns, C.A.M.; Kuerten, J.G.M.; de Waele, A.T.A.M.; Gijsman, H.M.

1985-01-01

The mutual friction between 3 He and 4 He II below 150 mK has been studied. Empirical relations for the adiabatic and the nonadiabatic flow properties of 3 He moving through 4 He have been determined using a dilution refrigerator with a single mixing chamber. The validity of the relations is verified by osmotic-pressure measurements and by measuring the properties of a double-mixing-chamber system. It is shown that superleak shunts have a strong effect on the flow characteristics. From the nonadiabatic flow properties an expression is derived for the mutual-friction-force density between 3 He and 4 He II. This has a strong resemblance to the Gorter-Mellink mutual-friction-force density between the normal and the superfluid components in pure 4 He II. It is speculated that the 3 He flow in our systems generates a 4 He vortex tangle, which leads to the observed mutual friction between 3 He and 4 He and also to a strong clamping of the 4 He to the walls

Observation of the Rotational Spectra of 4HeH+, 4HeD+, 3HeH+, and 3HeD+

International Nuclear Information System (INIS)

Matsushima, F.; Oka, T.; Takagi, K.

1997-01-01

Low J rotational transitions of 4 HeH + , 4 HeD + , 3 HeH + , and 3 HeD + were observed in the 2 endash 5THz region with a high-precision far-infrared spectrometer. Dunham coefficients Y kl and isotopically independent parameters U kl , Δ He kl , and Δ H kl were determined. In particular, Δ parameters with k=0 and l=1,2 were determined with unprecedented accuracy, and provide important information for breakdown of the Born-Oppenheimer approximation. The lowest J=1 left-arrow 0 transition of 4 HeH + observed at 2010.1839(2)GHz will be an important future probe for detecting this species in space. copyright 1997 The American Physical Society

Effect of primarily cultured human lung cancer-associated fibroblasts on radiosensitivity of lung cancer cells

International Nuclear Information System (INIS)

Ji Xiaoqin; Ji Jiang; Chen Yongbing; Shan Fang; Lu Xueguan

2014-01-01

Objective: To investigate the effect of human lung cancer-associated fibroblasts (CAF) on the radiosensitivity of lung cancer cells when CAF is placed in direct contact co-culture with lung cancer cells. Methods: Human lung CAF was obtained from fresh human lung adenocarcinoma tissue specimens by primary culture and subculture and was then identified by immunofluorescence staining. The CAF was placed in direct contact co-culture with lung cancer A 549 and H 1299 cells, and the effects of CAF on the radiosensitivity of A 549 and H 1299 cells were evaluated by colony-forming assay. Results: The human lung CAF obtained by adherent culture could stably grow and proliferate, and it had specific expression of α-smooth muscle actin, vimentin, and fibroblast activation protein,but without expression of cytokeratin-18. The plating efficiency (PE, %) of A 549 cells at 0 Gy irradiation was (20.0 ± 3.9)% when cultured alone versus (32.3 ± 5.5)% when co-cultured with CAF (t=3.16, P<0.05), and the PE of H 1299 cells at 0 Gy irradiation was (20.6 ± 3.1)% when cultured alone versus (35.2 ± 2.3)% when co-cultured with CAF (t=6.55, P<0.05). The cell survival rate at 2 Gy irradiation (SF 2 ) of A 549 cells was 0.727 ±0.061 when cultured alone versus 0.782 ± 0.089 when co-cultured with CAF (t=0.88, P>0.05), and the SF 2 of H 1299 cells was 0.692 ±0.065 when cultured alone versus 0.782 ± 0.037 when co-cultured with CAF (t=2.08, P>0.05). The protection enhancement ratios of human lung CAF for A 549 cells and H 1299 cells were 1.29 and 1.25, respectively. Conclusions: Human lung CAF reduces the radiosensitivity of lung cancer cells when placed in direct contact co-culture with them, and the radioprotective effect may be attributed to CAF promoting the proliferation of lung cancer cells. (authors)

The cross section of 3He(3He,2p)4He measured at solar energies

International Nuclear Information System (INIS)

Junker, M.; Arpesella, C.; Bellotti, E.; Broggini, C.; Corvisiero, P.; D'Alessandro, A.; Fiorentini, G.; Fubini, A.; Gervino, G.; Greife, U.; Gustavino, C.; Lambert, J.; Prati, P.; Rodney, W.S.; Rolfs, C.; Trautvetter, H.P.; Zavatarelli, S.

1999-01-01

We report on the status of the 3 He( 3 He,2p) 4 He experiment at the underground accelerator facility LUNA (Gran Sasso). The lowest projectile energies for the measured cross section correspond already to energies below the center of the solar Gamow peak (E 0 =22 keV). The data provide no evidence for the existence of a hypothetical resonance in the energy range investigated. Although no extrapolation is needed anymore (except for energies at the low-energy tail of the Gamow peak), the data must be corrected for the effects of electron screening, clearly observed the first time for the 3 He( 3 He,2p) 4 He reaction. The effects are however larger than expected and not understood, leading presently to the largest uncertainty on the quoted S(0) value for bare nuclides (= 5.40 MeVb)

Inactivated Tianjin strain, a novel genotype of Sendai virus, induces apoptosis in HeLa, NCI-H446 and Hep3B cells.

Science.gov (United States)

Chen, Jun; Han, Han; Wang, Bin; Shi, Liying

2016-07-01

The Sendai virus strain Tianjin is a novel genotype of the Sendai virus. In previous studies, ultraviolet-inactivated Sendai virus strain Tianjin (UV-Tianjin) demonstrated antitumor effects on human breast cancer cells. The aim of the present study was to investigate the in vitro antitumor effects of UV-Tianjin on the human cervical carcinoma HeLa, human small cell lung cancer NCI-H446 and human hepatocellular carcinoma Hep 3B cell lines, and the possible underlying mechanisms of these antitumor effects. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay revealed that UV-Tianjin treatment inhibited the proliferation of HeLa, NCI-H446 and Hep 3B cells in a dose- and time-dependent manner. Hoechst and Annexin V-fluorescein isothiocyanate/propidium iodide double staining indicated that UV-Tianjin induced dose-dependent apoptosis in all three cell lines with the most significant effect observed in the HeLa cell line. In the HeLa cell line, UV-Tianjin-induced apoptosis was further confirmed by the disruption of the mitochondria membrane potential and the activation of caspases, as demonstrated by fluorescent cationic dye and colorimetric assays, respectively. In addition, western blot analysis revealed that UV-Tianjin treatment resulted in significant upregulation of cytochrome c , apoptosis protease activating factor-1, Fas, Fas ligand and Fas-associated protein with death domain, and activated caspase-9, -8 and -3 in HeLa cells. Based on these results, it is hypothesized that UV-Tianjin exhibits anticancer activity in HeLa, NCI-H446 and Hep 3B cell lines via the induction of apoptosis. In conclusion, the results of the present study indicate that in the HeLa cell line, intrinsic and extrinsic apoptotic pathways may be involved in UV-Tianjin-induced apoptosis.

Stochastic rat lung dosimetry for inhaled radon progeny: a surrogate for the human lung for lung cancer risk assessment

Energy Technology Data Exchange (ETDEWEB)

Winkler-Heil, R.; Hofmann, W. [University of Salzburg, Division of Physics and Biophysics, Department of Materials Research and Physics, Salzburg (Austria); Hussain, M. [University of Salzburg, Division of Physics and Biophysics, Department of Materials Research and Physics, Salzburg (Austria); Higher Education Commission of Pakistan, Islamabad (Pakistan)

2015-05-15

Laboratory rats are frequently used in inhalation studies as a surrogate for human exposures. The objective of the present study was therefore to develop a stochastic dosimetry model for inhaled radon progeny in the rat lung, to predict bronchial dose distributions and to compare them with corresponding dose distributions in the human lung. The most significant difference between human and rat lungs is the branching structure of the bronchial tree, which is relatively symmetric in the human lung, but monopodial in the rat lung. Radon progeny aerosol characteristics used in the present study encompass conditions typical for PNNL and COGEMA rat inhalation studies, as well as uranium miners and human indoor exposure conditions. It is shown here that depending on exposure conditions and modeling assumptions, average bronchial doses in the rat lung ranged from 5.4 to 7.3 mGy WLM{sup -1}. If plotted as a function of airway generation, bronchial dose distributions exhibit a significant maximum in large bronchial airways. If, however, plotted as a function of airway diameter, then bronchial doses are much more uniformly distributed throughout the bronchial tree. Comparisons between human and rat exposures indicate that rat bronchial doses are slightly higher than human bronchial doses by about a factor of 1.3, while lung doses, averaged over the bronchial (BB), bronchiolar (bb) and alveolar-interstitial (AI) regions, are higher by about a factor of about 1.6. This supports the current view that the rat lung is indeed an appropriate surrogate for the human lung in case of radon-induced lung cancers. Furthermore, airway diameter seems to be a more appropriate morphometric parameter than airway generations to relate bronchial doses to bronchial carcinomas. (orig.)

Kinetic energy of He atoms in liquid 4He-3He mixtures

International Nuclear Information System (INIS)

Senesi, R.; Andreani, C.; Fielding, A.L.; Mayers, J.; Stirling, W.G.

2003-01-01

Deep inelastic neutron scattering measurements on liquid 3 He- 4 He mixtures in the normal phase have been performed on the VESUVIO spectrometer at the ISIS pulsed neutron source at exchanged wave vectors of about q≅120.0 A -1 . The neutron Compton profiles J(y) of the mixtures were measured along the T=1.96 K isotherm for 3 He concentrations, x, ranging from 0.1 to 1.0 at saturated vapor pressures. Values of kinetic energies of 3 He and 4 He atoms as a function of x, (x), were extracted from the second moment of J(y). The present determinations of (x) confirm previous experimental findings for both isotopes and, in the case of 3 He, a substantial disagreement with theory is found. In particular (x) for the 3 He atoms is found to be independent of concentration yielding a value 3 (x=0.1)≅12 K, much lower than the value suggested by the most recent theoretical estimates of approximately 19 K

Study of charge symmetry in 4He by simultaneous 4He(e,e'p)3H and 4He(e,e'n)3He measurements

International Nuclear Information System (INIS)

Spahn, M.; Kihm, T.; Knopfle, K.T.; Friedrich, J.; Voegler, N.; Schmitt, C.; Walther, V.H.; Unkelbach, M.; Hofmann, H.M.

1989-01-01

We have measured the ( 3 H+p) and ( 3 He+n) breakup of 4 He by 4 He(e,e'c) coincidence experiments at low momentum transfer (q∼0.39 fm -1 ) in the excitation energy region 22≤E x ≤36 MeV. Both channels were studied simultaneously by detecting the charged fragments c=p, 3 H, and 3 He in the same detector system. The measured 4 He(e,e'p) 3 H and 4 He(e,e'n) 3 He angular correlations as well as the deduced total 4 He(e,e'p) 3 H and 4 He(e,e'n) 3 He cross sections are reproduced by microscopic model calculations with a charge-symmetric nuclear Hamiltonian

Specific-heat measurements on dilute 3He-4He mixtures

International Nuclear Information System (INIS)

Zeeuw, H.C.M. van der.

1985-01-01

The author measured the specific heat of dilute 3 He- 4 He mixtures in the concentration range from X = 1 x 10 -3 to X = 3 x 10 -3 and in the temperature range from 100 mK to 600 mK. This has been done by means of a thermal relaxation method. This method provides some interesting features and is applied, to our knowledge, for the first time to dilute 3 He- 4 He mixtures. To reach the required temperature range for our experiments a 4 He circulating 3 He- 4 He dilution refrigerator has been constructed. The results confirm the deviation of the 3 He contribution to the specific heat from the ideal Fermi gas behaviour. (Auth.)

[Effect of cisplatin on the expression of Pokemon gene: experiment with different human lung cancer cells].

Science.gov (United States)

Zhao, Zhi-Hong; Wang, Sheng-Fa; Yu, Liang; Wang, Ju; Cong, De-Gang; Chang, Hao; Wang, Xue-Feng; Zhang, Tie-Wa; Zhang, Jian; Fu, Kai; Jiang, Jiu-Yang

2008-04-29

To investigate the correlation between Pokemon gene and cisplatin mechanism. Human lung adenocarcinoma cells of the lines A549 and AGZY83-a, human lung squamous carcinoma cells of the line HE-99, and human giant cell lung cancer cells of the line 95D were cultured and cisplatin was added into the medium. Other lung cancer cells of the above mentioned lines were cultured in the medium without cisplatin and were used as control groups. RT-PCR and Western blotting were used to detect the mRNA and protein expression of Pokemon. Pokemon mRNA and protein were expressed highly in all the 4 cell lines. The Pokemon gene expression did not changed significantly after cisplatin treatment groups. There were not significant differences in the mRNA and protein expression of Pokemon among the 4 experiment groups and the control groups (all P > 0.05). Cisplatin has no effect on the Pokemon gene expression of the human lung cancer cells.

Sulfation of chlorotyrosine and nitrotyrosine by human lung endothelial and epithelial cells: Role of the human SULT1A3

International Nuclear Information System (INIS)

Yasuda, Shin; Yasuda, Tomoko; Liu, Ming-Yih; Shetty, Sreerama; Idell, Steven; Boggaram, Vijayakumar; Suiko, Masahito; Sakakibara, Yoichi; Fu Jian; Liu, Ming-Cheh

2011-01-01

During inflammation, potent reactive oxidants formed may cause chlorination and nitration of both free and protein-bound tyrosine. In addition to serving as biomarkers of inflammation-mediated oxidative stress, elevated levels of chlorotyrosine and nitrotyrosine have been linked to the pathogenesis of lung and vascular disorders. The current study was designed to investigate whether the lung cells are equipped with mechanisms for counteracting these tyrosine derivatives. By metabolic labeling, chlorotyrosine O-[ 35 S]sulfate and nitrotyrosine O-[ 35 S]sulfate were found to be generated and released into the labeling media of human lung endothelial and epithelial cells labeled with [ 35 S]sulfate in the presence of added chlorotyrosine and nitrotyrosine. Enzymatic assays using the eleven known human cytosolic sulfotransferases (SULTs) revealed SULT1A3 as the enzyme responsible for catalyzing the sulfation of chlorotyrosine and nitrotyrosine. Reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated the expression of SULT1A3 in the lung endothelial and epithelial cells used in this study. Kinetic constants of the sulfation of chlorotyrosine and nitrotyrosine by SULT1A3 were determined. Collectively, these results suggest that sulfation by SULT1A3 in lung endothelial and epithelial cells may play a role in the inactivation and/or disposal of excess chlorotyrosine and nitrotyrosine generated during inflammation.

Galactic evolution of D and 3He including stellar production of 3He

International Nuclear Information System (INIS)

Dearborn, D.S.; Steigman, G.; Tosi, M.

1996-01-01

New stellar models which track the production and destruction of 3 He (and D) have been evolved for a range of stellar masses (0.65≤M/M circle-dot ≤100), metallicities (0.01≤Z/Z circle-dot ≤1), and initial (main-sequence) 3 He mass fractions (10 -5 ≤X 3,MS ≤10 -3 ). Armed with the 3 He yields from these stellar models we have followed the evolution of D and 3 He using a variety of chemical evolution models with and without infall of primordial or processed material. Production of new 3 He by the lower mass stars overwhelms any reasonable primordial contributions and leads to predicted abundances in the presolar nebula and/or the present interstellar medium in excess of the observationally inferred values. This result, which obtains even for zero primordial D and 3 He, and was anticipated by Rood, Steigman, ampersand Tinsley is insensitive to the choice of chemical evolution model; it is driven by the large 3 He yields from low-mass stars. In an attempt to ameliorate this problem we have considered a number of nonstandard models in which the yields from low-mass stars have been modified. Although several of these nonstandard models may be consistent with the 3 He data, they may be inconsistent with observations of 12 C/ 13 C, 18 O, and, most seriously the super- 3 He rich planetary nebulae. Even using the most extreme of these nonstandard models we obtain a generous upper bound to pregalactic 3 He:X 3P ≤3.2x10 -5 which, nonetheless, leads to a stringent lower bound to the universal density of nucleons. copyright 1996 The American Astronomical Society

Spin waves at the liquid 3He-4He interface

International Nuclear Information System (INIS)

Heff, A.; Candela, D.; Edwards, D.O.; Kumar, S.

1987-01-01

The properties of various interfaces in helium and, in particular, the interface between liquid 3 He and a solution of 3 He in 4 He, may be studied using spin waves. Assuming no transverse relaxation, the boundary condition for the transverse magnetization contains one complex kinetic coefficient, b. For the normal 3 He to 3 He- 4 He interface, b is related to the 3 He quasi-particle transmission probability antiτ, which we estimate from a simple model. A calculation of the spin wave absorption spectrum for a typical geometry shows that b and antiτ may be measured by NMR. Neither b nor antiτ is greatly affected when the pure 3 He enters the A phase, but both are strongly reduced in the B phase

A 3D human tissue-engineered lung model to study influenza A infection.

Science.gov (United States)

Bhowmick, Rudra; Derakhshan, Mina; Liang, Yurong; Ritchey, Jerry; Liu, Lin; Gappa-Fahlenkamp, Heather

2018-05-05

Influenza A virus (IAV) claims approximately 250,000-500,000 lives annually worldwide. Currently, there are a few in vitro models available to study IAV immunopathology. Monolayer cultures of cell lines and primary lung cells (2D cell culture) is the most commonly used tool, however, this system does not have the in vivo-like structure of the lung and immune responses to IAV as it lacks the three-dimensional (3D) tissue structure. To recapitulate the lung physiology in vitro, a system that contains multiple cell types within a 3D environment that allows cell movement and interaction, would provide a critical tool. In this study, as a first step in designing a 3D-Human Tissue-Engineering Lung Model (3D-HTLM), we described the 3D culture of primary human small airway epithelial cells (HSAEpCs), and determined the immunophenotype of this system in response to IAV infections. We constructed a 3D chitosan-collagen scaffold and cultured HSAEpCs on these scaffolds at air-liquid interface (ALI). These 3D cultures were compared with 2D-cultured HSAEpCs for viability, morphology, marker protein expression, and cell differentiation. Results showed that the 3D-cultured HSAEpCs at ALI yielded maximum viable cells and morphologically resembled the in vivo lower airway epithelium. There were also significant increases in aquaporin-5 and cytokeratin-14 expression for HSAEpCs cultured in 3D compared to 2D. The 3D culture system was used to study the infection of HSAEpCs with two major IAV strains, H1N1 and H3N2.The HSAEpCs showed distinct changes in marker protein expression, both at mRNA and protein levels, and the release of proinflammatory cytokines. This study is the first step in the development of the 3D-HTLM, which will have wide applicability in studying pulmonary pathophysiology and therapeutics development.

Saffold Virus Type 3 (SAFV-3) Persists in HeLa Cells

Science.gov (United States)

Himeda, Toshiki; Hosomi, Takushi; Okuwa, Takako; Muraki, Yasushi; Ohara, Yoshiro

2013-01-01

Saffold virus (SAFV) was identified as a human cardiovirus in 2007. Although several epidemiological studies have been reported, they have failed to provide a clear picture of the relationship between SAFV and human diseases. SAFV genotype 3 has been isolated from the cerebrospinal fluid specimen of patient with aseptic meningitis. This finding is of interest since Theiler’s murine encephalomyelitis virus (TMEV), which is the closely related virus, is known to cause a multiple sclerosis-like syndrome in mice. TMEV persistently infects in mouse macrophage cells in vivo and in vitro, and the viral persistence is essential in TMEV-induced demyelinating disease. The precise mechanism(s) of SAFV infection still remain unclear. In order to clarify the SAFV pathogenicity, in the present study, we studied the possibilities of the in vitro persistent infection of SAFV. The two distinct phenotypes of HeLa cells, HeLa-N and HeLa-R, were identified. In these cells, the type of SAFV-3 infection was clearly different. HeLa-N cells were lyticly infected with SAFV-3 and the host suitable for the efficient growth. On the other hand, HeLa-R cells were persistently infected with SAFV-3. In addition, the SAFV persistence in HeLa-R cells is independent of type I IFN response of host cells although the TMEV persistence in mouse macrophage cells depends on the response. Furthermore, it was suggested that SAFV persistence may be influenced by the expression of receptor(s) for SAFV infection on the host cells. The present findings on SAFV persistence will provide the important information to encourage the research of SAFV pathogenicity. PMID:23308162

Energy dependence of the 3He(3He,π+)6Li reaction

International Nuclear Information System (INIS)

Le Bornec, Y.; Hibou, F.; Bimbot, L.; Hennino, T.; Jourdain, J.C.; Reide, F.; Tatischeff, B.; Willis, N.; Aslanides, E.; Bergdolt, G.; Fassnacht, P.; Racca, C.; Boudard, A.; Bruge, G.; Lugol, J.C.

1983-01-01

The 3 He( 3 He, π + ) 6 Li reaction has been studied as a function of energy using the 3 He beam at SATURNE. Cross sections for the 6 Li ground state (1 + ) and 2.18 MeV (3 + ) levels have been obtained at 350, 420, 500 and 600 MeV incident energies at angles THETAsub(π)(lab)=15 0 and 40 0 . These results are compared with two theoretical predictions. (orig.)

Validity of apparent diffusion coefficient hyperpolarized 3He-MRI using MSCT and pulmonary function tests as references

DEFF Research Database (Denmark)

Diaz, Sandra; Casselbrant, Ingrid; Piitulainen, Eeva

2008-01-01

PURPOSE: To compare apparent diffusion coefficient (ADC) measurements from hyperpolarized (HP) helium ((3)He)-magnetic resonance imaging (MRI) with quantitative data from multislice Computed Tomography (CT) (MSCT) of the whole lungs and pulmonary function tests (PFT). MATERIALS AND METHODS: Twenty......-seven subjects, 22 with established emphysema and 5 with preclinical emphysema defined by PFT criteria, were examined with HP (3)He-MRI and MSCT. Mean age was 55 (+/-12) years, 18 female and 9 male. Mean ADC from (3)He-MRI was compared with emphysema index (EI), 15th percentile and mean lung density (MLD) values...... from MSCT. Both mean ADC and MSCT data were compared to PFT, especially percent of predicted diffusing capacity of carbon monoxide (%predicted DLCO), using Pearson's correlation test. RESULTS: Mean ADC and standard deviation values were 0.392+/-0.119 cm(2)/s for the established emphysema group and 0...

The search for high-energy deuterons in the 3He +3He reaction

International Nuclear Information System (INIS)

Pigeon, R.; Slobodrian, R.J.

1979-01-01

High-energy deuterons have been detected from the 3 He + 3 He reaction with a system sensitive to cross-sections of 0.6 nb sr -1 . Several tests have permitted to evaluate the small contribution of spurious events. The deuterons are kenematically consistent with the reaction 3 He + 3 He→ 2 H + 4 He + e + +ν, but the measured cross-section at 20deg laboratory is too high for a weak-interaction process; (1.3 +- 0.2) nb sr -1 . It might be due to an interaction of intermediate strength causing the decay of pp pairs ( 3 He) into deuterons. Other alternatives and the implications concerning fusion processes and the production of neutrinos in the sun are discussed in the text

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection.

Science.gov (United States)

Braian, Clara; Svensson, Mattias; Brighenti, Susanna; Lerm, Maria; Parasa, Venkata R

2015-10-05

Tuberculosis (TB) still holds a major threat to the health of people worldwide, and there is a need for cost-efficient but reliable models to help us understand the disease mechanisms and advance the discoveries of new treatment options. In vitro cell cultures of monolayers or co-cultures lack the three-dimensional (3D) environment and tissue responses. Herein, we describe an innovative in vitro model of a human lung tissue, which holds promise to be an effective tool for studying the complex events that occur during infection with Mycobacterium tuberculosis (M. tuberculosis). The 3D tissue model consists of tissue-specific epithelial cells and fibroblasts, which are cultured in a matrix of collagen on top of a porous membrane. Upon air exposure, the epithelial cells stratify and secrete mucus at the apical side. By introducing human primary macrophages infected with M. tuberculosis to the tissue model, we have shown that immune cells migrate into the infected-tissue and form early stages of TB granuloma. These structures recapitulate the distinct feature of human TB, the granuloma, which is fundamentally different or not commonly observed in widely used experimental animal models. This organotypic culture method enables the 3D visualization and robust quantitative analysis that provides pivotal information on spatial and temporal features of host cell-pathogen interactions. Taken together, the lung tissue model provides a physiologically relevant tissue micro-environment for studies on TB. Thus, the lung tissue model has potential implications for both basic mechanistic and applied studies. Importantly, the model allows addition or manipulation of individual cell types, which thereby widens its use for modelling a variety of infectious diseases that affect the lungs.

Thermodynamic properties of 3He--4He mixtures near Tlambda

International Nuclear Information System (INIS)

Kakizaki, A.; Satoh, T.

1976-01-01

In order to investigate 3 He impurity effects on the superfluid transition, measurements were made on the thermodynamic quantities, specific heat, thermal expansion coefficients, and pressure dependence of the lambda-transition temperature of three 3 He-- 4 He mixtures and pure 4 He in the neighborhood of the lambda-transition temperature under their saturated vapor pressure. Making use of these measured quantities, it is shown that the so-called Pippard--Buckingham--Fairbank relation holds for 3 He-- 4 He mixtures as well as for pure 4 He, at least in the temperature region of 10 -4 K less than or equal to absolute value (T - T/sub lambda/) less than or equal to 10 -2 K. Based on this, the 3 He impurity effects on the behavior of the specific heat near the lambda-transition are discussed

He3 cryostat

International Nuclear Information System (INIS)

Novichkova, L.G.; Kulikov, Yu.V.; Stashkova, N.M.

1992-01-01

An effective cryostat featuring a one-cycle He 3 refrigerator with a cryosorption pump is described. Gaseous He 3 in contained in a low-pressure vessel having a common wall with a liquid N 2 bath. Due to this, the gas is cooled before sorption and the sorption rate increases approximately by a factor of ten. The cryostat is provided with a needle valve which cuts off the ballast volume of the pipelines from the refrigerator. As a result, the pressure of He 3 vapours increases before condensation and with refrigerator dimensions selected properly, the liquefaction coefficient reaches approximately 80%. Test results of the cryostat and its specifications are presented. (Author)

Efficient 3He/4He separation in a nanoporous graphenylene membrane.

Science.gov (United States)

Qu, Yuanyuan; Li, Feng; Zhao, Mingwen

2017-08-16

Helium-3 is a precious noble gas, which is essential in many advanced technologies such as cryogenics, isotope labeling and nuclear weapons. The current imbalance of 3 He demand and supply shortage leads to the search for an efficient membrane with high performance for 3 He separation. In this study, based on first-principles calculations, we demonstrated that highly efficient 3 He harvesting can be achieved in a nanoporous graphenylene membrane with industrially-acceptable selectivity and permeance. The quantum tunneling effect leads to 3 He harvesting with high efficiency via kinetic sieving. Both the quantum tunneling effect and zero-point energy (ZPE) determine the 3 He/ 4 He separation via thermally-driven equilibrium sieving, where the ZPE effect dominates efficient 3 He/ 4 He separation between two reservoirs. The quantum effects revealed in this work suggest that the nanoporous graphenylene membrane is promising for efficient 3 He harvesting that can be exploited for industrial applications.

Hyperpolarized 3helium magnetic resonance ventilation imaging of the lung in cystic fibrosis: comparison with high resolution CT and spirometry

International Nuclear Information System (INIS)

McMahon, Colm J.; Dodd, Jonathan D.; Skehan, Stephen J.; Masterson, James B.; Hill, Catherine; Woodhouse, Neil; Wild, Jim M.; Fichele, Stan; Gallagher, Charles G.; Beek, Edwin J.R. van

2006-01-01

The purpose of this study was to compare hyperpolarized 3 helium magnetic resonance imaging ( 3 He MRI) of the lungs in adults with cystic fibrosis (CF) with high-resolution computed tomography (HRCT) and spirometry. Eight patients with stable CF prospectively underwent 3 He MRI, HRCT, and spirometry within 1 week. Three-dimensional (3D) gradient-echo sequence was used during an 18-s breath-hold following inhalation of hyperpolarized 3 He. Each lung was divided into six zones; 3 He MRI was scored as percentage ventilation per lung zone. HRCT was scored using a modified Bhalla scoring system. Univariate (Spearman rank) and multivariate correlations were performed between 3 He MRI, HRCT, and spirometry. Results are expressed as mean±SD (range). Spirometry is expressed as percent predicted. There were four men and four women, mean age=31.9±9 (20-46). Mean forced expiratory volume in 1 s (FEV) 1 =52%±29 (27-93). Mean 3 He MRI score=74%±25 (55-100). Mean HRCT score=48.8±24 (13.5-83). The correlation between 3 He MRI and HRCT was strong (R=±0.89, p 3 He MRI; 3 He MRI correlated better with FEV 1 and forced vital capacity (FVC) (R=0.86 and 0.93, p 3 He MRI correlates strongly with structural HRCT abnormalities and is a stronger correlate of spirometry than HRCT in CF. (orig.)

Second sound velocities in superfluid 3He-4He solutions

International Nuclear Information System (INIS)

Dikina, L.S.; Kotenev, G.Ya.; Rudavskij, Eh.Ya.

1978-01-01

The velocities of the second sound in the superfluid He 3 -He 4 solutions were measured by the pulse method in the range of temperatures from 1.3 K to Tsub(lambda) and for He 3 concentrations up to 13%.The results obtained supplemented by those available before give the complete description of the concentration and temperature dependences of the second sound velocity in superfluid He 3 -He 4 solutions. The comprehensive comparison of the experimental data on the velocity of the second sound with the theoretical calculations for the superfluid solutions with arbitrary content of He 3 is performed. The good agreement is found between experiment and the theory. The experimental data obtained are used for determination of the potential, which determines the properties of the superfluid solutions

Third sound resonance studies of 3He-4He mixture films

International Nuclear Information System (INIS)

Heinrichs, R.M.

1985-01-01

Third sound velocity and dissipation measurements were performed on 3 He- 4 He mixture films of 3.6 and 5.3 layers 4 He and up to 3 added layers 3 He in the temperature range 0.05-0.3 K using third sound resonance techniques. The 3 He concentration dependence of the velocity was in excellent agreement with a simple layered film model down to the lowest concentrations studied. At the higher 3 He concentrations, the velocity began to deviate from the model, rising above it. The concentration dependence of the dissipation showed some unique structure with a maximum at 0.3-0.5 layers added 3 He for both 4 He coverages. The third sound velocity in the low concentration mixture films had very little temperature dependence in the range studied. The dissipation, however, had some definite temperature dependent structure that evolved with concentration. This evolution was most pronounced at concentrations near the dissipation maximum and eventually became exponential at the higher coverages. Fitting these exponentials produced estimates of the difference in binding energy for the 3 He states in and on the film. In this higher 3 He concentration regime, a temperature dependence of the velocity was observed that began as a small peak at about 0.15 K and eventually became a step at the highest concentrations studied. The exponential nature of the dissipation became clouded when the velocity evolved into this last behavior

Autoradiographic visualization of muscarinic receptor subtypes in human and guinea pig lung

International Nuclear Information System (INIS)

Mak, J.C.; Barnes, P.J.

1990-01-01

Muscarinic receptor subtypes have been localized in human and guinea pig lung sections by an autoradiographic technique, using [3H](-)quinuclidinyl benzilate [( 3H]QNB) and selective muscarinic antagonists. [3H]QNB was incubated with tissue sections for 90 min at 25 degrees C, and nonspecific binding was determined by incubating adjacent serial sections in the presence of 1 microM atropine. Binding to lung sections had the characterization expected for muscarinic receptors. Autoradiography revealed that muscarinic receptors were widely distributed in human lung, with dense labeling over submucosal glands and airway ganglia, and moderate labeling over nerves in intrapulmonary bronchi and of airway smooth muscle of large and small airways. In addition, alveolar walls were uniformly labeled. In guinea pig lung, labeling of airway smooth muscle was similar, but in contrast to human airways, epithelium was labeled but alveolar walls were not. The muscarinic receptors of human airway smooth muscle from large to small airways were entirely of the M3-subtype, whereas in guinea pig airway smooth muscle, the majority were the M3-subtype with a very small population of the M2-subtype present. In human bronchial submucosal glands, M1- and M3-subtypes appeared to coexist in the proportions of 36 and 64%, respectively. In human alveolar walls the muscarinic receptors were entirely of the M1-subtype, which is absent from the guinea pig lung. No M2-receptors were demonstrated in human lung. The localization of M1-receptors was confirmed by direct labeling with [3H]pirenzepine. With the exception of the alveolar walls in human lung, the localization of muscarinic receptor subtypes on structures in the lung is consistent with known functional studies

Photodisintegration of 3H and 3He

International Nuclear Information System (INIS)

Faul, D.D.

1980-09-01

The photoneutron cross sections for 3 H and 3 He have been measured from threshold to approx. 25 MeV with monoenergetic photons from the annihilation in flight of fast positrons at the LLL Electron-Positron Linear Accelerator facility. These reactions include the two-body breakup of 3 H and the three-body breakup of both 3 H and 3 He; these measurements for 3 H are the first to span the energy region across the peaks of the cross sections. An efficient BF 3 -tube-and-paraffin neutron detector and high-pressure gaseous samples of several moles each (the activity of the 3 H sample was approx. 200,000 Ci) were employed in these measurements. Measurements on 16 O and 2 H also were performed to verify the absolute cross-section scale. The results, when compared with each other and with results for the two-body breakup cross section for 3 He from the literature, show that the two-body breakup cross sections for 3 H and 3 He have nearly the same shape, but the one for 3 He lies lower in magnitude; the three-body breakup cross section for 3 He lies higher in magnitude and is broader in the peak region and also rises less sharply from threshold than that for 3 H; and these measured differences between the cross sections for the breakup modes largely compensate in their sum, so that the total photon absorption cross sections for 3 H and 3 He are nearly the same in both size and shape at energies near and above their peaks. Theoretical results from the literature disagree with the experimental results to a certain extent over the entire photon-energy region for which the photoneutron cross sections were measured. 50 figures, 7 tables

The 3He Supply Problem

Energy Technology Data Exchange (ETDEWEB)

Kouzes, Richard T.

2009-05-01

One of the main uses for 3He is in gas proportional counters for neutron detection. Radiation portal monitors deployed for homeland security and non-proliferation use such detectors. Other uses of 3He are for research detectors, commercial instruments, well logging detectors, dilution refrigerators, for targets or cooling in nuclear research, and for basic research in condensed matter physics. The US supply of 3He comes almost entirely from the decay of tritium used in nuclear weapons by the US and Russia. A few other countries contribute a small amount to the world’s 3He supply. Due to the large increase in use of 3He for homeland security, the supply has dwindled, and can no longer meet the demand. This white paper reviews the problems of supply, utilization, and alternatives.

Dynamics of phase-separated 3He-4He films

International Nuclear Information System (INIS)

Kurihara, Susumu

1982-01-01

A froehlich-type Hamiltonian is derived for third sound and 3 He quasi particles in phase-separated double layer of superfluid 4 He and normal 3 He liquid. It is stressed that our system is unique and valuable in that characteristic parameters can be varied in a wide range, simply by adjusting the film thickness. The effect of fermion-boson coupling on the velocity and damping of the third sound is examined. It is predicted that a rather drastic change in the third sound spectrum will occur when the Fermi velocity of 3 He system and the third sound velocity are nearly the same. It is pointed out that the system under consideration may show a variety of interesting phenomena, in addition to the softening of the third sound. (author)

Heat pulses in dilute 3He-4He mixtures

International Nuclear Information System (INIS)

Husson, L.P.J.

1983-01-01

The propagation of heat pulses in dilute 3 He- 4 He mixtures under pressure along a tube, which is long compared to its diameter, is discussed. At high temperatures, where the excitations are in local equilibrium with one another, the propagation of heat pulses in the liquid is determined by the phenomenon of second sound, which is essentially a density wave in the excitation gas. The velocity and attenuation of second sound can be determined from the shape of the transmitted pulse. Measurements on the scattering and absorption of phonons are presented, together with a detailed description of the experimental technique and the electronic equipment. Measurements on the velocity and absorption of second sound are presented. From the results for the velocity of second sound in pure 4 He, values of the phonon and roton parameters are deduced. The velocity data in the mixtures have been used to calculate values of the effective mass of 3 He in superfluid 4 He. The results of these calculations have been compared with values of the effective mass obtained by other authors. The coefficient of second-sound absorption in pure 4 He is computed from theory, making use of the phonon and roton parameters calculated from the second-sound velocity data in pure 4 He. The experimental results on the scattering and absorption of phonons have been analysed. The empirical expressions for the scattering and absorption rates obtained from the diffusive phonon signals were compared with the results of the Baym-Ebner theory, and have also been used to calculate the coefficient of thermal conductivity in dilute 3 He- 4 He mixtures. (Auth.)

Analyzing Power of 3He(n, n)3He Between 1.60 and 5.54 MeV

International Nuclear Information System (INIS)

Esterline, J.; Tornow, W.

2013-01-01

We report the measurements of the analyzing power A y (θ) of n- 3 He elastic scattering with unprecedented accuracy and angular coverage at five incident neutron energies between 1.60 and 5.54 MeV. To this end, we employed the polarization-transfer reactions 3 H(p, n) 3 He and 2 H(d, n) 3 He at 0 ° for neutron generation and a recently developed high-pressure 3 He gas scintillator as an active target, enabling neutron-time-of-flight and 3 He recoil-energy determinations. We obtained simultaneously the neutron polarization with a 4 He-based polarimeter, capitalizing on the well-known n- 4 He A y (θ). Our n- 3 He A y (θ) data are compared to rigorous four-nucleon calculations using high-precision nucleon–nucleon potential models. The agreement between data and calculations is fair at the lower energies and becomes less satisfactory with increasing neutron energy. However, in comparison to the pure isotriplet p- 3 He system in the same energy region, the agreement between measured and calculated A y is much better for the mixed (isotriplet and isosinglet) n- 3 He system, indicating large and somewhat counterintuitive isospin effects. We note that the incorporation of a Δ-mediated three-nucleon force in calculations of n- 3 He scattering was found to affect A y (θ) negligibly, suggesting the need for the reconsideration of fundamental interactions in resolving the four-nucleon analyzing power puzzle first established about a decade ago in p- 3 He scattering. (author)

Cosmogenic 3He in detrital gold

Science.gov (United States)

Stuart, Finlay; Yakubovich, Olga; Caracedo, Ana; Nesterenok, Alexander

2017-04-01

Since the measurement of cosmogenic He in an alluvial diamond by McConville and Reynolds (1996) the application of cosmogenic noble gases to individual detrital grains to quantify surface processes has not been vigorously pursued. The likely low rate of diffusion of cosmogenic He in native metals, and their resistance to weathering and disintegration during erosion and transport, makes them a potential record of long-term Earth surface processes. In an effort to assess the extent that detrital refractory metals record the exposure history during transport and storage we have undertaken a reconnaissance study of the He isotope composition in 18 grains (2-200 mg) of native gold, copper, silver, and PtPd, Pt3Fe and OsIr alloys from alluvial placer deposits from around the world. 4He is dominantly the result of U and Th decay within the grains, or decay of 190Pt in the Pt-rich alloys. 3He is measurable in 13 grains, concentrations range up to 2.7E+6 atoms/g. 3He/4He are always in excess of the crustal radiogenic ratio, up to 306 Ra. Although nucleogenic 3He produced by (n,α) reactions on 6Li, and 3He from trapped hydrothermal fluids, are present, the majority of the 3He is cosmogenic in origin. Using newly calculated cosmogenic 3He production rates in heavy metals, and a determination of the effect of implantation based on the stopping distances of spallogenic 3He and 3H, the grains have 3Hecos concentrations that are equivalent to 0.35 to 1.5 Ma exposure at Earth's surface. In a study of detrital gold grains from several sites in Scotland we have found that 10 % have 3He concentrations that are significantly in excess of that generated since the Last Glacial Maximum. These studies demonstrate that, with refinement, cosmogenic 3He in refractory detrital minerals can be used to quantify sediment transport and storage on the 1-10 Ma timescale. P. McConville & J.H. Reynolds (1989). Geochim. Cosmochim. Acta, 53, 2365-75.

Annihilation of antiprotons at rest in 3He and 4He

International Nuclear Information System (INIS)

Balestra, F.; Bossolasco, S.; Bussa, M.P.; Busso, L.; Fava, L.; Ferrero, L.; Panzieri, D.; Piragino, G.; Tosello, F.; Bendiscioli, G.; Rotondi, R.; Salvini, P.; Zenoni, A.; Batusov, Y.A.; Bunyatov, S.A.; Falomkin, I.V.; Nichitiu, F.; Pontecorvo, G.B.; Sapozhnikov, M.G.; Tretyak, V.I.; Guaraldo, C.; Maggiora, A.; Haatuft, A.; Halsteinslid, A.; Myklebost, K.; Olsen, J.M.; Breivik, F.O.; Jacobsen, T.; Sorensen, S.O.

1987-01-01

At LEAR of CERN the annihilation of antiprotons stopping in 3 He and 4 He filling a self shunted streamer chamber in magnetic field has been studied. The relative probabilities of π - production in anti p 3 He and anti p 4 He annihilation events have been obtained. The ratio between the anti p annihilation probability on the neutron and on the proton for 3 He and 4 He has been deduced to be about half the value obtained for 2 H in bubble chamber experiments. The analysis of the results shows this difference cannot be ascribed only to the pion final state interaction or to the shadow effect of the nucleons in the nuclei. 9 refs.; 2 figs

(3He,xn), (3He,pxn) and (3He, fission) reactions on 206Pb between 80 and 200MeV

International Nuclear Information System (INIS)

Andre, C.; Gauvin, H.; Le Beyec, Y.; Porile, N.T.

1976-01-01

The reactions induced in 206 Pb by 3 He particles having energies between 80 and 200MeV have been studied. Excitation functions for ( 3 He,xn) with x=3 to 14 and for ( 3 He,pxn) with x=2 to 5 have been obtained. Angular distributions of fission fragments were measured at 100, 125, 150 and 175MeV and total fission cross-sections were deduced from the data. On the basis of these results, analysis is attempted to examine the characteristics of reaction mechanisms. From these results it is concluded that non-compound processes play an important role in the reactions. Two features are characteristic of these processes: large cross-sections for charged particle emission and angular distribution of fission fragments closed to isotropy in the laboratory system. In the energy range 25 to 45MeV/nucleon, a comparison was made between the present results and those from an experimental study of α-particle induced reactions on 206 Pb. Also a comparison was made with an α-nucleus collision model applied to 206 Pb. All the observations strongly suggest a breakup of the projectile 3 He followed by the interactions of the fragments with the target nucleus [fr

Hyperpolarized {sup 3}helium magnetic resonance ventilation imaging of the lung in cystic fibrosis: comparison with high resolution CT and spirometry

Energy Technology Data Exchange (ETDEWEB)

McMahon, Colm J.; Dodd, Jonathan D.; Skehan, Stephen J.; Masterson, James B. [St. Vincent' s University Hospital, Department of Radiology, Dublin (Ireland); Hill, Catherine; Woodhouse, Neil; Wild, Jim M.; Fichele, Stan [Royal Hallamshire Hospital, The Unit of Academic Radiology, University of Sheffield, Sheffield (United Kingdom); Gallagher, Charles G. [St. Vincent' s University Hospital, Department of National Referral Centre for Adult Cystic Fibrosis, Dublin (Ireland); Beek, Edwin J.R. van [Royal Hallamshire Hospital, The Unit of Academic Radiology, University of Sheffield, Sheffield (United Kingdom); University of Iowa, Department of Radiology, Carver College of Medicine, Iowa City, IA (United States)

2006-11-15

The purpose of this study was to compare hyperpolarized {sup 3}helium magnetic resonance imaging ({sup 3}He MRI) of the lungs in adults with cystic fibrosis (CF) with high-resolution computed tomography (HRCT) and spirometry. Eight patients with stable CF prospectively underwent {sup 3}He MRI, HRCT, and spirometry within 1 week. Three-dimensional (3D) gradient-echo sequence was used during an 18-s breath-hold following inhalation of hyperpolarized {sup 3}He. Each lung was divided into six zones; {sup 3}He MRI was scored as percentage ventilation per lung zone. HRCT was scored using a modified Bhalla scoring system. Univariate (Spearman rank) and multivariate correlations were performed between {sup 3}He MRI, HRCT, and spirometry. Results are expressed as mean{+-}SD (range). Spirometry is expressed as percent predicted. There were four men and four women, mean age=31.9{+-}9 (20-46). Mean forced expiratory volume in 1 s (FEV){sub 1}=52%{+-}29 (27-93). Mean {sup 3}He MRI score=74%{+-}25 (55-100). Mean HRCT score=48.8{+-}24 (13.5-83). The correlation between {sup 3}He MRI and HRCT was strong (R={+-}0.89, p<0.001). Bronchiectasis was the only independent predictor of {sup 3}He MRI; {sup 3}He MRI correlated better with FEV{sub 1} and forced vital capacity (FVC) (R=0.86 and 0.93, p<0.01, respectively) than HRCT (R={+-}0.72 and {+-}0.81, p<0.05, respectively). This study showed that {sup 3}He MRI correlates strongly with structural HRCT abnormalities and is a stronger correlate of spirometry than HRCT in CF. (orig.)

Study of the sup 3 H( sup 3 H, 2n) sup 4 He and sup 3 He( sup 3 He, 2p) sup 4 He reactions in the framework of three-cluster microscopic model

CERN Document Server

Vasilevsky, V S; Arickx, F; Broeckhove, J

2002-01-01

The reactions sup 3 H( sup 3 H, 2n) sup 4 He and sup 3 He( sup 3 He, 2p) sup 4 He are investigated within a fully microscopic cluster model featuring a three-cluster exit channel. A Hyperspherical Harmonics basis is used to describe the three-cluster continuum. The resulting astrophysical s-factor of both reactions is in good agreement with experimental data. Analysis of the low-energy scattering parameters reveals no evidence for a hidden resonance state would increase the cross-section of the reactions, and would help to resolve the solar neutrino problem.

MiR-564 functions as a tumor suppressor in human lung cancer by targeting ZIC3

Energy Technology Data Exchange (ETDEWEB)

Yang, Bin [Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079 (China); Jia, Lin [Department of Nephrology, The Central Hospital of Wuhan, Wuhan, Hubei 430079 (China); Guo, Qiaojuan [Department of Radiation Oncology, Fujian Provincial Cancer Hospital, Provincial Clinical College of Fujian Medical University, Fuzhou, Fujian 350000 (China); Ren, Hui; Hu, Desheng; Zhou, Xiaoyi; Ren, Qingrong [Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079 (China); Hu, Yanping, E-mail: huyp1989@163.com [Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079 (China); Xie, Tao, E-mail: xietao930@hotmail.com [Department of Radiation Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079 (China)

2015-11-27

Although miR-564 was reported to be dysregulated in human malignancy, the function and mechanism of miR-564 in tumorigenesis remains unknown. In the present study, we found that miR-564 frequently downregulated in lung cancer cells and significantly inhibited cell proliferation, cell cycle progression, motility, and the tumorigenicity of lung cancer cells. Moreover, we identified zic family member 3 (ZIC3) as a direct target of miR-564. ZIC3 overexpression impaired the suppressive effects of miR-564 on the capacity of lung cancer cells for proliferation and motility. Finally, we detected the expression level of miR-564 and ZIC3 protein in tissue specimens, and found a significant negative correlation between them. Patients with low levels of miR-564 showed a poorer overall survival. Taken together, our present study revealed the tumor suppressor role of miR-564, indicating restoration of miR-564 as a potential therapeutic strategy for the treatment of lung cancer. - Highlights: • MiR-564 inhibits cancer cell proliferation, cell cycle progression, migration, and invasion. • miR-564 suppresses the tumorigenicity of lung cancer cell in vivo. • ZIC3 is a direct and functional target of miR-564. • The expression of miR-564 was negatively correlated with ZIC3 protein in tumors. • Both low miR-564 and high ZIC3 was associated with tumor stage and prognosis.

MiR-564 functions as a tumor suppressor in human lung cancer by targeting ZIC3

International Nuclear Information System (INIS)

Yang, Bin; Jia, Lin; Guo, Qiaojuan; Ren, Hui; Hu, Desheng; Zhou, Xiaoyi; Ren, Qingrong; Hu, Yanping; Xie, Tao

2015-01-01

Although miR-564 was reported to be dysregulated in human malignancy, the function and mechanism of miR-564 in tumorigenesis remains unknown. In the present study, we found that miR-564 frequently downregulated in lung cancer cells and significantly inhibited cell proliferation, cell cycle progression, motility, and the tumorigenicity of lung cancer cells. Moreover, we identified zic family member 3 (ZIC3) as a direct target of miR-564. ZIC3 overexpression impaired the suppressive effects of miR-564 on the capacity of lung cancer cells for proliferation and motility. Finally, we detected the expression level of miR-564 and ZIC3 protein in tissue specimens, and found a significant negative correlation between them. Patients with low levels of miR-564 showed a poorer overall survival. Taken together, our present study revealed the tumor suppressor role of miR-564, indicating restoration of miR-564 as a potential therapeutic strategy for the treatment of lung cancer. - Highlights: • MiR-564 inhibits cancer cell proliferation, cell cycle progression, migration, and invasion. • miR-564 suppresses the tumorigenicity of lung cancer cell in vivo. • ZIC3 is a direct and functional target of miR-564. • The expression of miR-564 was negatively correlated with ZIC3 protein in tumors. • Both low miR-564 and high ZIC3 was associated with tumor stage and prognosis.

Transforming growth factor-β1 induces expression of human coagulation factor XII via Smad3 and JNK signaling pathways in human lung fibroblasts.

Science.gov (United States)

Jablonska, Ewa; Markart, Philipp; Zakrzewicz, Dariusz; Preissner, Klaus T; Wygrecka, Malgorzata

2010-04-09

Coagulation factor XII (FXII) is a liver-derived serine protease involved in fibrinolysis, coagulation, and inflammation. The regulation of FXII expression is largely unknown. Transforming growth factor-beta1 (TGF-beta1) is a multifunctional cytokine that has been linked to several pathological processes, including tissue fibrosis by modulating procoagulant and fibrinolytic activities. This study investigated whether TGF-beta1 may regulate FXII expression in human lung fibroblasts. Treatment of human lung fibroblasts with TGF-beta1 resulted in a time-dependent increase in FXII production, activation of p44/42, p38, JNK, and Akt, and phosphorylation and translocation into the nucleus of Smad3. However, TGF-beta1-induced FXII expression was repressed only by the JNK inhibitor and JNK and Smad3 antisense oligonucleotides but not by MEK, p38, or phosphoinositide 3-kinase blockers. JNK inhibition had no effect on TGF-beta1-induced Smad3 phosphorylation, association with Smad4, and its translocation into the nucleus but strongly suppressed Smad3-DNA complex formation. FXII promoter analysis revealed that the -299/+1 region was sufficient for TGF-beta1 to induce FXII expression. Sequence analysis of this region detected a potential Smad-binding element at position -272/-269 (SBE-(-272/-269)). Chromatin immunoprecipitation and streptavidin pulldown assays demonstrated TGF-beta1-dependent Smad3 binding to SBE-(-272/-269). Mutation or deletion of SBE-(-272/-269) substantially reduced TGF-beta1-mediated activation of the FXII promoter. Clinical relevance was demonstrated by elevated FXII levels and its co-localization with fibroblasts in the lungs of patients with acute respiratory distress syndrome. Our results show that JNK/Smad3 pathway plays a critical role in TGF-beta1-induced FXII expression in human lung fibroblasts and implicate its possible involvement in pathological conditions characterized by elevated TGF-beta1 levels.

Thermometry in 3He

International Nuclear Information System (INIS)

Richardson, R.C.

1977-01-01

Some of the methods used in measuring the temperatures of the transitions in 3 He are reviewed. Although noise thermometers, γ-ray anisotropy thermometers and even Pt NMR thermometers are satisfactory for furnishing temperature standards, they are not satisfactory secondary thermometers. The susceptibility of CMN and various 3 He properties make more precise and quicker thermometric indications. (Auth.)

Δ excitation in 3He and 4He by photons

International Nuclear Information System (INIS)

Maruyama, Koichi.

1993-06-01

The use of the 3 He and 4 He photodisintegration reactions in the study of the excitation, propagation, and decay of the Δ (1232) in these nuclei is proposed. By using the data obtained with TAGX for both photon absorption on neutron-proton pairs and complete photodisintegration, we find no compelling evidence for the change of the Δ property in the 3 He and 4 He nuclei. It is also proposed to use nuclear photodisintegration in the search for the excitation of the higher-mass nucleon resonances whose absence in the total photon-absorption cross sections in nuclei is reported recently. (author)

Interactive lung segmentation in abnormal human and animal chest CT scans

International Nuclear Information System (INIS)

Kockelkorn, Thessa T. J. P.; Viergever, Max A.; Schaefer-Prokop, Cornelia M.; Bozovic, Gracijela; Muñoz-Barrutia, Arrate; Rikxoort, Eva M. van; Brown, Matthew S.; Jong, Pim A. de; Ginneken, Bram van

2014-01-01

Purpose: Many medical image analysis systems require segmentation of the structures of interest as a first step. For scans with gross pathology, automatic segmentation methods may fail. The authors’ aim is to develop a versatile, fast, and reliable interactive system to segment anatomical structures. In this study, this system was used for segmenting lungs in challenging thoracic computed tomography (CT) scans. Methods: In volumetric thoracic CT scans, the chest is segmented and divided into 3D volumes of interest (VOIs), containing voxels with similar densities. These VOIs are automatically labeled as either lung tissue or nonlung tissue. The automatic labeling results can be corrected using an interactive or a supervised interactive approach. When using the supervised interactive system, the user is shown the classification results per slice, whereupon he/she can adjust incorrect labels. The system is retrained continuously, taking the corrections and approvals of the user into account. In this way, the system learns to make a better distinction between lung tissue and nonlung tissue. When using the interactive framework without supervised learning, the user corrects all incorrectly labeled VOIs manually. Both interactive segmentation tools were tested on 32 volumetric CT scans of pigs, mice and humans, containing pulmonary abnormalities. Results: On average, supervised interactive lung segmentation took under 9 min of user interaction. Algorithm computing time was 2 min on average, but can easily be reduced. On average, 2.0% of all VOIs in a scan had to be relabeled. Lung segmentation using the interactive segmentation method took on average 13 min and involved relabeling 3.0% of all VOIs on average. The resulting segmentations correspond well to manual delineations of eight axial slices per scan, with an average Dice similarity coefficient of 0.933. Conclusions: The authors have developed two fast and reliable methods for interactive lung segmentation in

Specific heat of 4He and 3He--4He mixtures at their lambda transition

International Nuclear Information System (INIS)

Gasparini, F.M.; Moldover, M.R.

1975-01-01

We have measured the specific heat near the lambda transition of pure 4 He and of five 3 He-- 4 He mixtures up to a mole fraction of 0.39 3 He in 4 He. Our data for 4 He confirm the results of Ahlers revealing an asymmetry in the exponents above and below T/sub lambda/ when the specific heat is represented by a simple-power-law temperature dependence. Our results for these exponents (α = 0.012 plus-or-minus 0.002 and α' = -0.012 plus-or-minus 0.004) differ somewhat from Ahlers's. Our results can be reconciled with the requirement of scaling (α = α') only by supposing substantial contributions to C/sub p/ are made by singular correction terms to a simple power law. The measured specific heat of the mixtures richest in 3 He appears to be finite, continuous, and cusped at the lambda line. These qualitative features have been termed ''renormalization'' by Fisher. An analysis of our mixture data with a power-law temperature dependence does not yield a fully renormalized exponent, but rather an effective exponent. Derivatives at the lambda line were used to calculate the specific heat along paths of constant pressure and constant relative chemical potential.This specific heat behaves very much like C/sub p/ of pure 4 He, this behavior supporting the idea of universality for the specific-heat exponents. It is also true that the same asymmetry in the branches above and below T/sub lambda/ which is []bserved in pure 4 He is retained in the mixtures. The persistence of the asymmetry of C/subp//sub phi/ as one moves along the lambda line towards increasing 3 He concentration (at the saturated vapor pressure of the mixtures) is analogous to the persistence of the asymmetry of C/subp/ as one moves along the lambda line towards increasing pressure in pure 4 He

Annihilation of antiprotons at rest in 3He and 4He

International Nuclear Information System (INIS)

Balestra, F.; Bossolasco, S.; Bussa, M.P.; Busso, L.; Fava, L.; Ferrero, L.; Panzieri, D.; Piragino, G.; Tosello, F.; Bendiscioli, G.; Rotondi, A.; Salvini, P.; Zenoni, A.; Batusov, Yu.A.; Bunyatov, S.A.; Falomkin, I.V.; Nichitiu, F.; Pontecorvo, G.B.; Sapozhnikov, M.G.; Tretyak, V.I.; Guaraldo, C.; Maggiora, A.; Haatuft, A.; Halsteinslid, A.; Myklebost, K.; Olsen, J.M.; Breivik, F.O.; Jacobsen, T.; Soerensen, S.O.

1987-01-01

At LEAR of CERN the annihilation of antiprotons, stopping in 3 He and 4 He filling a self-shunted streamer chamber in a magnetic field, has been studied. The charged-particle multiplicities have been measured and the relative probabilities of π - production in anti p 3 He and anti p 4 He annihilation events have been obtained. The ratio between the anti p annihilation probability on the neutron and the proton for 3 He and 4 He has been deduced to be about half the value obtained for 2 H in bubble chamber experiments. The analysis of the results shows that this difference cannot be due only to the pion final-state interaction or to the shadow effect of the nucleons of the nuclei. The probability of anti p annihilation at rest on a proton bound in the nucleus results to be twice as high as that on a bound neutron, showing the dominance of annihilation in the I=0 isospin states. (orig.)

Regional and temporal variations in CO2/3He, 3He/4He and δ13C along the North Anatolian Fault Zone, Turkey

International Nuclear Information System (INIS)

Leeuw, G.A.M. de; Hilton, D.R.; Guelec, N.; Mutlu, H.

2010-01-01

New He and C relative abundance, isotope and concentration results from nine geothermal locations situated along an 800-km transect of the North Anatolian Fault Zone (NAFZ), Turkey, that were monitored during the period November 2001-November 2004, are reported. The geothermal waters were collected every 3-6 months to study possible links between temporal geochemical variations and seismic activity along the NAFZ. At the nine sample locations, the He isotope ratios range from 0.24 to 2.3R A , δ 13 C values range from -4.5 to +5.8 per mille, and CO 2 / 3 He ratios range from 5 x 10 9 to 5 x 10 14 . The following geochemical observations are noted: (1) the highest 3 He/ 4 He ratios are found near the Galatean volcanic region, in the central section of the NAFZ, (2) at each of the nine sample locations, the 3 He/ 4 He ratios are generally constant; however, CO 2 / 3 He ratios and He contents both show one order of magnitude variability, and δ 13 C values show up to ∼4 per mille variability, and (3) at all locations (except Resadiye), δ 13 C values show positive correlations with CO 2 contents. The results indicate that at least three processes are necessary to account for the geochemical variations: (1) binary mixing between crustal and mantle-derived volatiles can explain the general characteristics of 3 He/ 4 He ratios, δ 13 C values, and CO 2 / 3 He ratios at the nine sample locations; (2) preferential degassing of He from the geothermal waters is responsible for variations in CO 2 / 3 He values and He contents at each sample location; and (3) CO 2 dissolution followed by calcite precipitation is responsible for variations in CO 2 contents and δ 13 C values at most locations. For each of the geochemical parameters, anomalies are defined in the temporal record by values that fall outside two standard deviations of average values at each specific location. Geochemical anomalies that may be related to seismic activity are recorded on June 28, 2004 at Yalova

First clinical evaluation of radioimmunoimaging using anti-human lung cancer monoclonal antibodies

International Nuclear Information System (INIS)

Zhou Qian

1991-01-01

Anti-human large cell lung cancer monoclonal antibodies (McAb) 2E3 and 6D1 were produced in the laboratory. Immunohistochemical studies and radiobinding assay showed these antibodies possessed high specificity against lung cancer cells. 28 patients with lung masses were investigated with 131 I-labeled McAb 6D1 and/or 2E3 scintigraphy. 19 of them were histologically proven and 13 were diagnosed primary lung carcinoma. Radioimmunoimaging visualized 10/13 of the primary lung cancers with a detection rate of 77%. Only 1 case of the non-cancer patients and a false localization, giving a true negative rate of 83%. Pathologically the squamous cell lung carcinoma had the highest localization and the small cell lung carcinoma next, but the detection rate was 100% for both. The adenocarcinoma of lung was less sensitive to these McAbs, with a detection rate of only 33% (1 of 3 cases). We conclude that radioimmunoimaging with anti-human large cell lung cancer McAbs is more specific and effective in detecting primary lung cancers and differentiating lung masses than with antibodies against other tumor associated antigens

3He(d,p)4He reaction calculation with three-body Faddeev equations

International Nuclear Information System (INIS)

Oryu, S.; Uzu, E.; Sunahara, H.; Yamada, T.; Tabaru, G.; Hino, T.

1998-01-01

In order to investigate the 3 He-n-p system as a three-body problem, we have formulated 3 He-n and 3 H-p effective potentials using both a microscopic treatment and a phenomenological approach. In the microscopic treatment, potentials are generated by means of the resonating group method (RGM) based on the Minnesota nucleon-nucleon potential. These potentials are converted into separable form by means of the microscopic Pauli correct (MPC) method. The MPC potentials are properly formulated to avoid Pauli forbidden states. The phenomenological potentials are obtained by modifying parameters of the EST approximation to the Paris nucleon-nucleon potential, such that they fit the low-energy 3 He-n, 3 H-p, and 3 He-p phase shifts. Therefore, they describe the 3 He-n differential cross section, the polarization observables, and the energy levels of 4 He. The 3 He-n-p Faddeev equations are solved numerically. We reproduce correctly the ground state and the first excited state of 5 Li. Furthermore, the Paris-type potential is used to investigate the 3 He(d,p) 4 He reaction at a deuteron bombarding energy of 270 MeV, where the system is treated as a three-body problem. Results for the polarized and unpolarized differential cross sections demonstrate convergence of the Born series. (orig.)

Mobility of ions trapped on vortex lines in pure 4He and 3He--4He solutions

International Nuclear Information System (INIS)

Ostermeier, R.M.; Glaberson, W.I.

1976-01-01

Measurements have been made of the mobility of positive and negative ions trapped on vortex lines in pure 4 He and dilute 3 He-- 4 He solutions over the temperature range 1.6 greater than T greater than 0.3 K. In pure 4 He below about 0.7 K, several new effects not seen at higher temperatures are observed and are not easily explained with existing theories. Most notable are an enhanced broadening of the ion pulse and a rapid increase in the mobility with decreasing temperature. Measurements of the electric field dependence of the drift velocity in pure 4 He at low temperatures show a limiting velocity for sufficiently large fields. This behavior can be explained using a simple resonance theory. The inverse mobility data for solutions show sharp increases at certain critical temperatures, which are interpreted as being associated with the condensation of 3 He atoms onto the vortex cores. The dependence of the critical temperature on the bulk 3 He concentration is found to be in good agreement with a simple condensation theory. An extension of arguments used in this theory to lower temperatures leads to the picture of a 3 He-rich core growing with decreasing temperature, consistent with our lower temperature experimental data

Analytic Intermodel Consistent Modeling of Volumetric Human Lung Dynamics.

Science.gov (United States)

Ilegbusi, Olusegun; Seyfi, Behnaz; Neylon, John; Santhanam, Anand P

2015-10-01

Human lung undergoes breathing-induced deformation in the form of inhalation and exhalation. Modeling the dynamics is numerically complicated by the lack of information on lung elastic behavior and fluid-structure interactions between air and the tissue. A mathematical method is developed to integrate deformation results from a deformable image registration (DIR) and physics-based modeling approaches in order to represent consistent volumetric lung dynamics. The computational fluid dynamics (CFD) simulation assumes the lung is a poro-elastic medium with spatially distributed elastic property. Simulation is performed on a 3D lung geometry reconstructed from four-dimensional computed tomography (4DCT) dataset of a human subject. The heterogeneous Young's modulus (YM) is estimated from a linear elastic deformation model with the same lung geometry and 4D lung DIR. The deformation obtained from the CFD is then coupled with the displacement obtained from the 4D lung DIR by means of the Tikhonov regularization (TR) algorithm. The numerical results include 4DCT registration, CFD, and optimal displacement data which collectively provide consistent estimate of the volumetric lung dynamics. The fusion method is validated by comparing the optimal displacement with the results obtained from the 4DCT registration.

Effective interactions in dilute mixtures of 3He in 4He

International Nuclear Information System (INIS)

Hsu, W.; Pines, D.

1985-01-01

Nonlocal pseudopotentials which describe the effective interaction between 3 He quasiparticles, and between these quasiparticles and the background 4 He liquid, are obtained as a function of concentration and pressure by generalizing the Aldrich--Pines pseudopotentials for pure 3 He and 4 He to dilute mixtures. The hierarchy of physical effects which determine these pseudopotentials is established. Interaction-induced short-range correlations are the dominant physical feature; next in order of importance is the greater zero point motion associated with the replacement of a 4 He atom by a 3 He atom, while spin-duced ''Pauli principle'' correlations play a significantly smaller, albeit still important role. We find a consistent trend in the change of the effective direct quasiparticle interactions with increasing concentration, and show how the Aldrich-Pines pseudopotentials for pure 3 He quasiparticles represent a natural extension of our results for dilute mixtures. Our calculated nonlocal pseudopotential for 3 He quasiparticles is qualitatively similar to that proposed by Bardeen, Baym, and Pines; it changes sign at somewhat lower momentum transfers than the BBP result, varies little with concentration, and provides a physical basis for understanding the BBP result. The effective interaction between quasiparticles of parallel spin, here determined for the first time, is essentially repulsive in the very dilute limit; as the concentration increases, it becomes increasingly attractive at low momentum transfers, and resembles closely that between antiparallel spin quasiparticles at 5% concentration. The concentration-dependent transport properties calculated from these pseudopotentials (which involve only one phenomenological parameter) are in good agreement with experiment at saturated vapor pressure (SVP), 10 atm, and 20 atm

Hyperpolarized 3He magnetic resonance imaging: Preliminary evaluation of phenotyping potential in chronic obstructive pulmonary disease

International Nuclear Information System (INIS)

Mathew, Lindsay; Kirby, Miranda; Etemad-Rezai, Roya; Wheatley, Andrew; McCormack, David G.; Parraga, Grace

2011-01-01

Rationale and objectives: Emphysema and small airway obstruction are the pathological hallmarks of chronic obstructive pulmonary disease (COPD). The aim of this pilot study in a small group of chronic obstructive pulmonary disease (COPD) patients was to quantify hyperpolarized helium-3 ( 3 He) magnetic resonance imaging (MRI) functional and structural measurements and to explore the potential role for 3 He MRI in detecting the lung structural and functional COPD phenotypes. Materials and methods: We evaluated 20 ex-smokers with stage I (n = 1), stage II (n = 9) and stage III COPD (n = 10). All subjects underwent same-day plethysmography, spirometry, 1 H MRI and hyperpolarized 3 He MRI at 3.0 T. 3 He ventilation defect percent (VDP) was generated from 3 He static ventilation images and 1 H thoracic images and the 3 He apparent diffusion coefficient (ADC) was derived from diffusion-weighted MRI. Results: Based on the relative contribution of normalized ADC and VDP, there was evidence of a predominant 3 He MRI measurement in seven patients (n = 3 mainly ventilation defects or VDP dominant (VD), n = 4 mainly increased ADC or ADC dominant (AD)). Analysis of variance (ANOVA) showed significantly lower ADC for subjects with predominantly elevated VDP (p = 0.02 compared to subjects with predominantly elevated ADC; p = 0.008 compared to mixed group) and significantly decreased VDP for subjects with predominantly elevated ADC (p = 0.003, compared to mixed group). Conclusion: In this small pilot study, a preliminary analysis shows the potential for 3 He MRI to categorize or phenotype COPD ex-smokers, providing good evidence of feasibility for larger prospective studies.

Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model

Directory of Open Access Journals (Sweden)

Israel L. Medeiros

2012-09-01

Full Text Available OBJECTIVE: Experimental studies on lung preservation have always been performed using animal models. We present ex vivo lung perfusion as a new model for the study of lung preservation. Using human lungs instead of animal models may bring the results of experimental studies closer to what could be expected in clinical practice. METHOD: Brain-dead donors whose lungs had been declined by transplantation teams were used. The cases were randomized into two groups. In Group 1, Perfadex®was used for pulmonary preservation, and in Group 2, LPDnac, a solution manufactured in Brazil, was used. An ex vivo lung perfusion system was used, and the lungs were ventilated and perfused after 10 hours of cold ischemia. The extent of ischemic-reperfusion injury was measured using functional and histological parameters. RESULTS: After reperfusion, the mean oxygenation capacity was 405.3 mmHg in Group 1 and 406.0 mmHg in Group 2 (p = 0.98. The mean pulmonary vascular resistance values were 697.6 and 378.3 dyn·s·cm-5, respectively (p =0.035. The mean pulmonary compliance was 46.8 cm H20 in Group 1 and 49.3 ml/cm H20 in Group 2 (p =0.816. The mean wet/dry weight ratios were 2.06 and 2.02, respectively (p=0.87. The mean Lung Injury Scores for the biopsy performed after reperfusion were 4.37 and 4.37 in Groups 1 and 2, respectively (p = 1.0, and the apoptotic cell counts were 118.75/mm² and 137.50/mm², respectively (p=0.71. CONCLUSION: The locally produced preservation solution proved to be as good as Perfadex®. The clinical use of LPDnac may reduce costs in our centers. Therefore, it is important to develop new models to study lung preservation.

Coupling between Solid 3He on Aerogel and Superfluid 3He in the Low Temperature Limit

International Nuclear Information System (INIS)

Bradley, D. I.; Fisher, S. N.; Guenault, A. M.; Haley, R. P.; Pickett, G. R.; Tsepelin, V.; Whitehead, R. C. V.; Skyba, P.

2006-01-01

We have cooled liquid 3He contained in a 98% open aerogel sample surrounded by bulk superfluid 3He-B at zero pressure to below 120 μK. The aerogel sample is placed in a quasiparticle blackbody radiator cooled by a Lancaster-style nuclear cooling stage to ∼200 μK. We monitor the temperature of the 3He inside the blackbody radiator using a vibrating wire resonator. We find that reducing the magnetic field on the aerogel sample causes substantial cooling of all the superfluid inside the blackbody radiator. We believe this is due to the demagnetization of the solid 3He layers on the aerogel strands. This system has potential for achieving extremely low temperatures in the confined fluid

An image acquisition and registration strategy for the fusion of hyperpolarized helium-3 MRI and x-ray CT images of the lung

International Nuclear Information System (INIS)

Ireland, Rob H; Woodhouse, Neil; Hoggard, Nigel; Swinscoe, James A; Foran, Bernadette H; Hatton, Matthew Q; Wild, Jim M

2008-01-01

The purpose of this ethics committee approved prospective study was to evaluate an image acquisition and registration protocol for hyperpolarized helium-3 magnetic resonance imaging ( 3 He-MRI) and x-ray computed tomography. Nine patients with non-small cell lung cancer (NSCLC) gave written informed consent to undergo a free-breathing CT, an inspiration breath-hold CT and a 3D ventilation 3 He-MRI in CT position using an elliptical birdcage radiofrequency (RF) body coil. 3 He-MRI to CT image fusion was performed using a rigid registration algorithm which was assessed by two observers using anatomical landmarks and a percentage volume overlap coefficient. Registration of 3 He-MRI to breath-hold CT was more accurate than to free-breathing CT; overlap 82.9 ± 4.2% versus 59.8 ± 9.0% (p 3 He-MRI and CT to be acquired with similar breath holds and body position through the use of a birdcage 3 He-MRI body RF coil and an inspiration breath-hold CT. Fusion of 3 He-MRI to CT may be useful for the assessment of patients with lung diseases.

Effects of Δ-isobar degrees of freedom on the reactions 3He(n,γ)4He and 3He(p,e+νe)4He at low-energy

International Nuclear Information System (INIS)

Schiavilla, R.

1991-01-01

The cross sections of the radiative 3 He(n,γ) 4 He and weak 3 He(p,e + ν e ) 4 He capture reactions at thermal neutron and keV proton energies have been calculated with the Variational Monte Carlo method. The ground state and low-energy continuum wave functions have been determined variationally from a realistic Hamiltonian, and include both nucleon and Δ-isobar degrees of freedom. The electroweak transition operator contains one- and two-body components in the N + Δ Hilbert space

New estimates for human lung dimensions

International Nuclear Information System (INIS)

Kennedy, Christine; Sidavasan, Sivalal; Kramer, Gary

2008-01-01

Full text: The currently used lung dimensions in dosimetry were originally estimated in the 1940s from Army recruits. This study provides new estimates of lung dimensions based on images acquired from a sample from the general population (varying age and sex). Building accurate models, called phantoms, of the human lung requires that the spatial dimensions (length, width, and depth) be quantified, in addition to volume. Errors in dose estimates may result from improperly sized lungs as the counting efficiency of externally mounted detectors (e.g., in a lung counter) is dependent on the position of internally deposited radioactive material (i.e., the size of the lung). This study investigates the spatial dimensions of human lungs. Lung phantoms have previously been made in one of two sizes. The Lawrence Livermore National Laboratory Torso Phantom (LLNL) has deep, short lungs whose dimensions do not comply well with the data published in Report 23 (Reference Man) issued by the International Commission on Radiological Protection (ICRP). The Japanese Atomic Energy Research Institute Torso Phantom(JAERI), has longer, shallower lungs that also deviate from the ICRP values. However, careful examination of the ICRP recommended values shows that they are soft. In fact, they have been dropped from the ICRP's Report 89 which updates Report 23. Literature surveys have revealed a wealth of information on lung volume, but very little data on the spatial dimensions of human lungs. Better lung phantoms need to be constructed to more accurately represent a person so that dose estimates may be quantified more accurately in view of the new, lower, dose limits for occupationally exposed workers and the general public. Retrospective chest images of 60 patients who underwent imaging of the chest- lungs as part of their healthy persons occupational screening for lung disease were chosen. The chosen normal lung images represent the general population). Ages, gender and weight of the

A human lung xenograft mouse model of Nipah virus infection.

Directory of Open Access Journals (Sweden)

Gustavo Valbuena

2014-04-01

Full Text Available Nipah virus (NiV is a member of the genus Henipavirus (family Paramyxoviridae that causes severe and often lethal respiratory illness and encephalitis in humans with high mortality rates (up to 92%. NiV can cause Acute Lung Injury (ALI in humans, and human-to-human transmission has been observed in recent outbreaks of NiV. While the exact route of transmission to humans is not known, we have previously shown that NiV can efficiently infect human respiratory epithelial cells. The molecular mechanisms of NiV-associated ALI in the human respiratory tract are unknown. Thus, there is an urgent need for models of henipavirus infection of the human respiratory tract to study the pathogenesis and understand the host responses. Here, we describe a novel human lung xenograft model in mice to study the pathogenesis of NiV. Following transplantation, human fetal lung xenografts rapidly graft and develop mature structures of adult lungs including cartilage, vascular vessels, ciliated pseudostratified columnar epithelium, and primitive "air" spaces filled with mucus and lined by cuboidal to flat epithelium. Following infection, NiV grows to high titers (10(7 TCID50/gram lung tissue as early as 3 days post infection (pi. NiV targets both the endothelium as well as respiratory epithelium in the human lung tissues, and results in syncytia formation. NiV infection in the human lung results in the production of several cytokines and chemokines including IL-6, IP-10, eotaxin, G-CSF and GM-CSF on days 5 and 7 pi. In conclusion, this study demonstrates that NiV can replicate to high titers in a novel in vivo model of the human respiratory tract, resulting in a robust inflammatory response, which is known to be associated with ALI. This model will facilitate progress in the fundamental understanding of henipavirus pathogenesis and virus-host interactions; it will also provide biologically relevant models for other respiratory viruses.

A comparative study of the progression of radiation pulmonary injury in C57BL/6J and C3H/HeN mice

International Nuclear Information System (INIS)

Liu Ying; Li Yang; Peng Ruiyun; Wang Shuming; Gao Yabing; Ma Junjie; Song Liangwen

2007-01-01

Objective: To compare the course of radiation pulmonary injury in C57BL/6J and C3H/HeN mice. Methods: C57BL/6J and C3H/HeN mice irradiated with 20 Gy 60 Co gamma rays were used as animal models. Sirius red staining and hydroxyproline measurement were used to detect the distribution of type I and III collagens and the content of pulmonary hydroxyproline. Immunohistochemistry was used to observe the changes of the expressions of fibronectin (FN) and laminin (LN) and alpha-smooth muscle actin (α-SMA) in the lung. Results: The changes in the lungs in C57BL/6J mice were as follows: the pathological changes undergone interstitial pneumonia, proliferation and fibrosis; significant increase of collagen deposition; FN increased rapidly to a significantly higher level at 1 and 3 months after irradiation than that in the control (P<0.01), and then decreased gradually to a normal level at 6 month after irradiation; LN elevated gradually after irradiation; the expressions of α-SMA were more intense than that in C3H/HeN mice. The changes in the lungs in C3H/HeN mice were as follows: the pathological changes mainly developed institial pneumonitis; no significant changes of FN expression was observed after irradiation compared to that in the control; LN increased significantly at 1 and 3 months after irradiation, and then decreased gradually. Conclusions: The models of radiation pulmonary fibrosis-sensitive and-resistant were established by irradiation with gamma rays, C57BL/6J mice developed late radiation pulmonary fibrosis, and were characterized by the significant accumulation of collagen. C3H/HeN mice did not develop radiation pulmonary fibrosis. (authors)

Aerosolized 3-bromopyruvate inhibits lung tumorigenesis without causing liver toxicity.

Science.gov (United States)

Zhang, Qi; Pan, Jing; North, Paula E; Yang, Shoua; Lubet, Ronald A; Wang, Yian; You, Ming

2012-05-01

3-Bromopyruvate, an alkylating agent and a well-known inhibitor of energy metabolism, has been proposed as a specific anticancer agent. However, the chemopreventive effect of 3-bromopyruvate in lung tumorigenesis has not been tested. In this study, we investigated the chemopreventive activity of 3-bromopyruvate in a mouse lung tumor model. Benzo(a)pyrene was used to induce lung tumors, and 3-bromopyruvate was administered by oral gavage to female A/J mice. We found that 3-bromopyruvate significantly decreased tumor multiplicity and tumor load by 58% and 83%, respectively, at a dose of 20 mg/kg body weight by gavage. Due to the known liver toxicity of 3-bromopyruvate in animal models given large doses of 3-bromopyruvate, confirmed in this study, we decided to test the chemopreventive activity of aerosolized 3-bromopyruvate in the same lung tumor model. As expected, aerosolized 3-bromopyruvate similarly significantly decreased tumor multiplicity and tumor load by 49% and 80%, respectively, at a dose of 10 mg/mL by inhalation. Interestingly, the efficacy of aerosolized 3-bromopyruvate did not accompany any liver toxicity indicating that it is a safer route of administering this compound. Treatment with 3-bromopyruvate increased immunohistochemical staining for cleaved caspase-3, suggesting that the lung tumor inhibitory effects of 3-bromopyruvate were through induction of apoptosis. 3-Bromopyruvate also dissociated hexokinase II from mitochondria, reduced hexokinase activity, and blocked energy metabolism in cancer cells, finally triggered cancer cell death and induced apoptosis through caspase-3, and PARP in human lung cancer cell line. The ability of 3-bromopyruvate to inhibit mouse lung tumorigenesis, in part through induction of apoptosis, merits further investigation of this compound as a chemopreventive agent for human lung cancer.

Progression of emphysema in a 12-month hyperpolarized 3He-MRI study: lacunarity analysis provided a more sensitive measure than standard ADC analysis

DEFF Research Database (Denmark)

Diaz, Sandra; Casselbrant, Ingrid; Piitulainen, Eeva

2009-01-01

RATIONALE AND OBJECTIVES: Inhaled hyperpolarized (3)He magnetic resonance (MR) imaging has been used to measure alveolar size in patients with emphysema. The aim of this study was to test the hypothesis that (3)He MR images could be used to develop a biomarker of emphysema progression. MATERIALS...... AND METHODS: Twelve healthy controls and 18 patients with emphysema (eight current smokers, 10 ex-smokers) were imaged at baseline and 6 and 12 months. An additional nine subjects with alpha-1 antitrypsin deficiency (four with emphysema, six without symptoms) were also imaged at baseline and at 6 months. Each...... subject was imaged at two lung volumes: functional residual capacity (FRC) and FRC plus 15% of total lung capacity. Means and standard deviations of apparent diffusion coefficients (ADCs) were calculated from coronal images of the entire lung and correlated with pulmonary function test results...

Proteomic investigation into betulinic acid-induced apoptosis of human cervical cancer HeLa cells.

Science.gov (United States)

Xu, Tao; Pang, Qiuying; Zhou, Dong; Zhang, Aiqin; Luo, Shaman; Wang, Yang; Yan, Xiufeng

2014-01-01

Betulinic acid is a pentacyclic triterpenoid that exhibits anticancer functions in human cancer cells. This study provides evidence that betulinic acid is highly effective against the human cervical cancer cell line HeLa by inducing dose- and time-dependent apoptosis. The apoptotic process was further investigated using a proteomics approach to reveal protein expression changes in HeLa cells following betulinic acid treatment. Proteomic analysis revealed that there were six up- and thirty down-regulated proteins in betulinic acid-induced HeLa cells, and these proteins were then subjected to functional pathway analysis using multiple analysis software. UDP-glucose 6-dehydrogenase, 6-phosphogluconate dehydrogenase decarboxylating, chain A Horf6-a novel human peroxidase enzyme that involved in redox process, was found to be down-regulated during the apoptosis process of the oxidative stress response pathway. Consistent with our results at the protein level, an increase in intracellular reactive oxygen species was observed in betulinic acid-treated cells. The proteins glucose-regulated protein and cargo-selection protein TIP47, which are involved in the endoplasmic reticulum pathway, were up-regulated by betulinic acid treatment. Meanwhile, 14-3-3 family proteins, including 14-3-3β and 14-3-3ε, were down-regulated in response to betulinic acid treatment, which is consistent with the decrease in expression of the target genes 14-3-3β and 14-3-3ε. Furthermore, it was found that the antiapoptotic bcl-2 gene was down-regulated while the proapoptotic bax gene was up-regulated after betulinic acid treatment in HeLa cells. These results suggest that betulinic acid induces apoptosis of HeLa cells by triggering both the endoplasmic reticulum pathway and the ROS-mediated mitochondrial pathway.

Giant asymmetry of separation and homogenization processes in solid 3He-4He solutions

International Nuclear Information System (INIS)

Grigor'ev, V.N.; Majdanov, V.A.; Penzev, A.A.; Polev, A.V.; Rubets, S.P.; Rudavskij, Eh.Ya.; Rybalko, A.S.; Syrnikov, E.V.

2005-01-01

The kinetics of the processes of separation and homogenization of solid 3 He- 4 He solutions is compared by using the precision barometry. The experiments were made with the initial specimens of three types: weak 3 He- 4 He and 4 He- 3 He solutions and concentrated 3 He- 4 He ones. It is found that the homogenization rate at the initial stage may be more than 500 times higher that the rate of separation. This is the case for all types of the solutions studied. The appreciable rate of phase separation in the concentrated solutions where, according to the modern concepts, impurity atoms in quantum crystals should be localized, suggests that in such conditions there is a new unknown mechanism of mass-transfer, while the fast homogenization points to a nondiffusion nature of the process

TUSC3 induces autophagy in human non-small cell lung cancer cells through Wnt/?-catenin signaling

OpenAIRE

Peng, Yun; Cao, Jun; Yao, Xiao-Yi; Wang, Jian-Xin; Zhong, Mei-Zuo; Gan, Ping-Ping; Li, Jian-Huang

2017-01-01

We investigated the effects of tumor suppressor candidate 3 (TUSC3) on autophagy in human non-small cell lung cancer (NSCLC) cells. A total of 118 NSCLC patients (88 males and 30 females) who underwent surgery at our institute were enrolled in the study. Immunohistochemical analysis revealed that TUSC3 protein expression was lower in NSCLC specimens than adjacent normal tissue. Correspondingly, there was greater methylation of TUSC3 in NSCLC than adjacent normal tissue. After transient transf...

Overlap integrals of model wave functions of 4He and 3He,3H nuclei

International Nuclear Information System (INIS)

Voloshin, N.I.; Levshin, E.B.; Fursa, A.D.

1990-01-01

Overlap integrals of wave functions 4 He nucleus and 3 He and 3 H nuclei are calculated. Two types of model wave functions are used to describe the structure of nuclei. The wace function is taken as a product of the one-particle Gaussian functions of the Gaussian type in the second case

Hyperpolarized {sup 3}He magnetic resonance imaging: Preliminary evaluation of phenotyping potential in chronic obstructive pulmonary disease

Energy Technology Data Exchange (ETDEWEB)

Mathew, Lindsay, E-mail: lmathew@imaging.robarts.ca [Imaging Research Laboratories, Robarts Research Institute, London (Canada); Department of Medical Biophysics, University of Western Ontario, London (Canada); Kirby, Miranda, E-mail: mkirby@imaging.robarts.ca [Imaging Research Laboratories, Robarts Research Institute, London (Canada); Department of Medical Biophysics, University of Western Ontario, London (Canada); Etemad-Rezai, Roya, E-mail: Roya.EtemadRezai@lhsc.on.ca [Department of Medical Imaging, University of Western Ontario (Canada); Wheatley, Andrew, E-mail: awheat@imaging.robarts.ca [Imaging Research Laboratories, Robarts Research Institute, London (Canada); McCormack, David G., E-mail: David.McCormack@lhsc.on.ca [Imaging Research Laboratories, Robarts Research Institute, London (Canada); Division of Respirology, Department of Medicine, University of Western Ontario, London (Canada); Lawson Health Research Institute, London (Canada); Parraga, Grace, E-mail: gep@imaging.robarts.ca [Imaging Research Laboratories, Robarts Research Institute, London (Canada); Department of Medical Biophysics, University of Western Ontario, London (Canada); Department of Medical Imaging, University of Western Ontario (Canada); Lawson Health Research Institute, London (Canada)

2011-07-15

Rationale and objectives: Emphysema and small airway obstruction are the pathological hallmarks of chronic obstructive pulmonary disease (COPD). The aim of this pilot study in a small group of chronic obstructive pulmonary disease (COPD) patients was to quantify hyperpolarized helium-3 ({sup 3}He) magnetic resonance imaging (MRI) functional and structural measurements and to explore the potential role for {sup 3}He MRI in detecting the lung structural and functional COPD phenotypes. Materials and methods: We evaluated 20 ex-smokers with stage I (n = 1), stage II (n = 9) and stage III COPD (n = 10). All subjects underwent same-day plethysmography, spirometry, {sup 1}H MRI and hyperpolarized {sup 3}He MRI at 3.0 T. {sup 3}He ventilation defect percent (VDP) was generated from {sup 3}He static ventilation images and {sup 1}H thoracic images and the {sup 3}He apparent diffusion coefficient (ADC) was derived from diffusion-weighted MRI. Results: Based on the relative contribution of normalized ADC and VDP, there was evidence of a predominant {sup 3}He MRI measurement in seven patients (n = 3 mainly ventilation defects or VDP dominant (VD), n = 4 mainly increased ADC or ADC dominant (AD)). Analysis of variance (ANOVA) showed significantly lower ADC for subjects with predominantly elevated VDP (p = 0.02 compared to subjects with predominantly elevated ADC; p = 0.008 compared to mixed group) and significantly decreased VDP for subjects with predominantly elevated ADC (p = 0.003, compared to mixed group). Conclusion: In this small pilot study, a preliminary analysis shows the potential for {sup 3}He MRI to categorize or phenotype COPD ex-smokers, providing good evidence of feasibility for larger prospective studies.

Determination of the π3He3H coupling constant

International Nuclear Information System (INIS)

Nichitiu, F.; Sapozhnikov, M.G.

1977-01-01

Despersion relations for the real part of the antisymmetric amplitude of the π +-3 He scattering have been used in order to determine the π 3 He 3 H coupling constant. The coupling constant value determined by this method is larger than the elementary pion-nucleon coupling constant, but is in good agreement with the value obtained by another method. The obtained value is f 2 sub(π 3 He 3 H) = 0.12+-0.01. Shown is the importance of using the Coulomb corrections for dispersion relation calculations because the value of π 3 He 3 H coupling constant obtained with corrected total cross sections is larger by about 0.014 than the one obtained without these corrections. The best energy ranges for future π 3 He experiments are commented

Antimicrobial actions of the human epididymis 2 (HE2 protein isoforms, HE2alpha, HE2beta1 and HE2beta2

Directory of Open Access Journals (Sweden)

French Frank S

2004-08-01

Full Text Available Abstract Background The HE2 gene encodes a group of isoforms with similarities to the antimicrobial beta-defensins. We demonstrated earlier that the antimicrobial activity of HE2 proteins and peptides is salt resistant and structure dependent and involves permeabilization of bacterial membranes. In this study, we further characterize the antimicrobial properties of HE2 peptides in terms of the structural changes induced in E. coli and the inhibition of macromolecular synthesis. Methods E. coli treated with 50 micro g/ml of HE2alpha, HE2beta1 or HE2beta2 peptides for 30 and 60 min were visualized using transmission and scanning electron microscopy to investigate the impact of these peptides on bacterial internal and external structure. The effects of HE2alpha, HE2beta1 and HE2beta2 on E. coli macromolecular synthesis was assayed by incubating the bacteria with 2, 10 and 25 micro g/ml of the individual peptides for 0–60 min and measuring the incorporation of the radioactive precursors [methyl-3H]thymidine, [5-3H]uridine and L-[4,5-3H(N]leucine into DNA, RNA and protein. Statistical analyses using Student's t-test were performed using Sigma Plot software. Values shown are Mean ± S.D. Results E. coli treated with HE2alpha, HE2beta1 and HE2beta2 peptides as visualized by transmission electron microscopy showed extensive damage characterized by membrane blebbing, thickening of the membrane, highly granulated cytoplasm and appearance of vacuoles in contrast to the smooth and continuous membrane structure of the untreated bacteria. Similarly, bacteria observed by scanning electron microscopy after treating with HE2alpha, HE2beta1 or HE2beta2 peptides exhibited membrane blebbing and wrinkling, leakage of cellular contents, especially at the dividing septa, and external accumulation of fibrous materials. In addition, HE2alpha, HE2beta1 and HE2beta2 peptides inhibited E. coli DNA, RNA and protein synthesis. Conclusions The morphological changes observed

Expression analysis of asthma candidate genes during human and murine lung development.

Science.gov (United States)

Melén, Erik; Kho, Alvin T; Sharma, Sunita; Gaedigk, Roger; Leeder, J Steven; Mariani, Thomas J; Carey, Vincent J; Weiss, Scott T; Tantisira, Kelan G

2011-06-23

Little is known about the role of most asthma susceptibility genes during human lung development. Genetic determinants for normal lung development are not only important early in life, but also for later lung function. To investigate the role of expression patterns of well-defined asthma susceptibility genes during human and murine lung development. We hypothesized that genes influencing normal airways development would be over-represented by genes associated with asthma. Asthma genes were first identified via comprehensive search of the current literature. Next, we analyzed their expression patterns in the developing human lung during the pseudoglandular (gestational age, 7-16 weeks) and canalicular (17-26 weeks) stages of development, and in the complete developing lung time series of 3 mouse strains: A/J, SW, C57BL6. In total, 96 genes with association to asthma in at least two human populations were identified in the literature. Overall, there was no significant over-representation of the asthma genes among genes differentially expressed during lung development, although trends were seen in the human (Odds ratio, OR 1.22, confidence interval, CI 0.90-1.62) and C57BL6 mouse (OR 1.41, CI 0.92-2.11) data. However, differential expression of some asthma genes was consistent in both developing human and murine lung, e.g. NOD1, EDN1, CCL5, RORA and HLA-G. Among the asthma genes identified in genome wide association studies, ROBO1, RORA, HLA-DQB1, IL2RB and PDE10A were differentially expressed during human lung development. Our data provide insight about the role of asthma susceptibility genes during lung development and suggest common mechanisms underlying lung morphogenesis and pathogenesis of respiratory diseases.

Phase equilibrium in a polarized saturated 3He-4He mixture

International Nuclear Information System (INIS)

Rodrigues, A.; Vermeulen, G.

1997-01-01

We present experimental results on the phase equilibrium of a saturated 3 He- 4 He mixture, which has been cooled to a temperature of 10-15 mK and polarized in a 4 He circulating dilution refrigerator to a stationary polarization of 15 %, 7 times higher than the equilibrium polarization in the external field of 7 T. The pressure dependence of the polarization enhancement in the refrigerator shows that the molar susceptibilities of the concentrated and dilute phase of a saturated 3 He- 4 He mixture are equal at p = 2.60 ± 0.04 bar. This result affects the Fermi liquid parameters of the dilute phase. The osmotic pressure in the dilute phase has been measured as a function of the polarization of the coexisting concentrated phase up to 15 %. We find that the osmotic pressure at low polarization ( < 7 % ) agrees well with thermodynamics using the new Fermi liquid parameters of the dilute phase

Structure properties of the 3He-4He mixture at T = O K

International Nuclear Information System (INIS)

Boronat, J.; Polls, A.; Fabrocini, A.

1993-01-01

The spatial structure properties of 3 He- 4 He mixtures at T = O K are investigated using the hypernetted-chain formalism. The variational wave function used to describe the ground-state of the mixture is a simple generalization of the trial wave functions for pure phases and contains two- and three-body correlations. The elementary diagrams are taken into account by means of an extension of the scaling approximation to the mixtures. The two-body distribution (g (α,β) (r)) and the structure functions (S (α,β) (k)) together with the different spin-spin distribution functions for the 3 He component in the mixture are analyzed for several concentrations of 3 He. Two sum-rules, for the direct and the exchange part of the g (3,3) (r), are used to ascertain the importance of the full treatment of the Fermi statistics in the calculation. The statistical correlations are found responsible for the main differences between the several components of the distribution function. Due to its low concentration, 3 He behaves as a quasi-free Fermi gas, as far as the statistical correlations are concerned, although it is strongly correlated with the 4 He atoms through the interatomic potential

Spontaneous and radiation induced cell death in HeLa S3 human carcinoma

International Nuclear Information System (INIS)

Zaric, B.; Milosavljevic, B.; Radojcic, M.

2001-01-01

Radiation biologists have classified radiation-induced cell death based on cell proliferative capacity to either mitotic or interphase death. Cytologists have revealed two morphologically and biochemically diverse forms of cell death, apoptosis and necrosis. While the knowledge of the former is already well exploited by radiologists, cell susceptibility to apoptosis and necrosis is still under investigation. We studied characteristics of spontaneous cell death, and dose dependence and time course of radiation-induced cell death of human uterine cervix epitheloid carcinoma HeLaS 3 in culture. Cells were irradiated with 2-40 Gy of γ-rays. The effect on growth, viability, morphology and genomic DNA structure were followed 24-72 h after irradiation. Cell viability was evaluated by trypan-blue exclusion assay and cell morphology by in situ DNA staining with propidium iodide. Cell genomic DNA fragmentation pattern was determined by electrophoresis on 2% agarose gels. At all cell densities 25-35% cells were PI positive and their DNA was fragmented to a high molecular size (≥20 kbp), but the internucleosomal ladder was not observed. A significant decrease in viability to 33% was observed 72 h post 40 Gy irradiation. It corresponded to 55% of PI positive cells. A smear of smaller DNA fragments (0.1-1 kbp), 24 h after 10-20 Gy irradiation was considered as proof that the dominant form of radiation-induced cell death was necrosis. It was concluded that the dominant form of radiation-induced cell death in HeLaS 3 population was necrosis and the radiation dose which caused 50% of cell death after 72 h (termed ND 50 ) was between 30-40 Gy. (author)

Experimental considerations on producing highly polarized liquid 3He in a matrix of solid 4He

International Nuclear Information System (INIS)

Greenberg, A.S.; Hebral, B.; Papoular, M.; Beal-Monod, M.T.

1980-01-01

Two experiments are briefly reviewed in which droplets of 3 He were formed in solid 4 He. These experiments indicate such conditions are favorable for the production of quasi-stable highly polarized liquid 3 He. A solid solution of dilute 3 He in 4 He is proposed as a promising system to produce experimentally realizable highly polarized liquid 3 He using the Castaing-Nozieres decompression

Anomalous Quasiparticle Reflection from the Surface of a ^{3}He-^{4}He Dilute Solution.

Science.gov (United States)

Ikegami, Hiroki; Kim, Kitak; Sato, Daisuke; Kono, Kimitoshi; Choi, Hyoungsoon; Monarkha, Yuriy P

2017-11-10

A free surface of a dilute ^{3}He-^{4}He liquid mixture is a unique system where two Fermi liquids with distinct dimensions coexist: a three-dimensional (3D) ^{3}He Fermi liquid in the bulk and a two-dimensional (2D) ^{3}He Fermi liquid at the surface. To investigate a novel effect generated by the interaction between the two Fermi liquids, the mobility of a Wigner crystal of electrons formed on the free surface of the mixture is studied. An anomalous enhancement of the mobility, compared with the case where the 3D and 2D systems do not interact with each other, is observed. The enhancement is explained by the nontrivial reflection of 3D quasiparticles from the surface covered with the 2D ^{3}He system.

(3)He pO2 mapping is limited by delayed-ventilation and diffusion in chronic obstructive pulmonary disease.

Science.gov (United States)

Marshall, Helen; Parra-Robles, Juan; Deppe, Martin H; Lipson, David A; Lawson, Rod; Wild, Jim M

2014-03-01

Lung pO2 mapping with (3)He MRI assumes that the sources of signal decay with time during a breath-hold are radiofrequency depolarization and oxygen-dependent T1 relaxation, but the method is sensitive to other sources of spatio-temporal signal change such as diffusion. The purpose of this work was to assess the use of (3)He pO2 mapping in patients with chronic obstructive pulmonary disease. Ten patients with moderate to severe chronic obstructive pulmonary disease were scanned with a 3D single breath-hold pO2 mapping sequence. Images showed signal increasing over time in some lung regions due to delayed ventilation during breath-hold. Regions of physically unrealistic negative pO2 values were seen in all patients, and regional mean pO2 values of -0.3 bar were measured in the two patients most affected by delayed ventilation (where mean time to signal onset was 3-4 s). Movement of gas within the lungs during breath-hold causes regional changes in signal over time that are not related to oxygen concentration, leading to erroneous pO2 measurements using the linear oxygen-dependent signal decay model. These spatio-temporal sources of signal change cannot be reliably separated at present, making pO2 mapping using this methodology unreliable in chronic obstructive pulmonary disease patients with significant bullous emphysema or delayed ventilation. Copyright © 2013 Wiley Periodicals, Inc.

3He Abundances in Planetary Nebulae

Science.gov (United States)

Guzman-Ramirez, Lizette

2017-10-01

Determination of the 3He isotope is important to many fields of astrophysics, including stellar evolution, chemical evolution, and cosmology. The isotope is produced in stars which evolve through the planetary nebula phase. Planetary nebulae are the final evolutionary phase of low- and intermediate-mass stars, where the extensive mass lost by the star on the asymptotic giant branch is ionised by the emerging white dwarf. This ejecta quickly disperses and merges with the surrounding ISM. 3He abundances in planetary nebulae have been derived from the hyperfine transition of the ionised 3He, 3He+, at the radio rest frequency 8.665 GHz. 3He abundances in PNe can help test models of the chemical evolution of the Galaxy. Many hours have been put into trying to detect this line, using telescopes like the Effelsberg 100m dish of the Max Planck Institute for Radio Astronomy, the National Radio Astronomy Observatory (NRAO) 140-foot telescope, the NRAO Very Large Array, the Arecibo antenna, the Green Bank Telescope, and only just recently, the Deep Space Station 63 antenna from the Madrid Deep Space Communications Complex.

Performance of a mass spectrometer for determining low tritium levels from 3He/4He measurements

International Nuclear Information System (INIS)

Lynch, M.C.F.; Kay, D.J.

1981-01-01

The Micromass 3000 mass spectrometer is based on Clarke's 3 He/ 4 He instrument of McMaster University. The principal special features of the MM3000 are: (i) The double collector system, which uses an electron multiplier for the small 3 He + ion beams and a conventional Faraday detector for 4 He + ; (ii) The high sensitivity ion source; and (iii) The low-volume analyser tube employing static vacuum techniques. The sensitivity, resolution and background of the system are designed to permit measurement of 3 He and 4 He levels of the order of 10 -12 cm 3 STP and 10 -6 cm 3 STP, respectively, with a precision of 0.5%, although smaller quantities are detectable. The inherent resolution of the instrument is well in excess of 2000, but a resolution of 600-1000 is normally used for the 3 He + collector as this is sufficient to separate 3 He + from any interfering HD + or H + 3 ions. At this resolution 900 ions per second of 3 He + ions are obtained from 10 -12 cm 3 STP of 3 He. This is the size of 3 He samples obtained from water samples containing tritium at levels around 200 tritium units (1 tritium unit (TU) = IT/10 18 H). At lower tritium levels the 3 He + signal is correspondingly reduced, but the same techniques (described below) allow signals from 1-TU samples (5 ions/s) to be measured with good precision. The limit of detection is reached with quantities of 3 He in the region of 10 -15 cm 3 STP and tritium levels of around 0.1 TU. (author)

Growth and dissolution of liquid 3He droplets in solid 4He matrix

International Nuclear Information System (INIS)

Gan'shin, A.N.; Grigor'ev, V.N.; Majdanov, V.A.; Penzev, A.A.; Rudavskij, Eh.Ya.; Rybalko, A.S.

2000-01-01

The phase separation kinetics of solid 3 He - 4 He mixtures was investigated using pressure measurements in the conditions when the two-phase system formed consists of concentrated phase liquid droplets (almost pure 3 He) in the dilute phase crystal matrix (almost pure 4 He). It is shown that the liquid droplet growth may be described by a sum of two exponential processes with small and large time contacts as cooling down step by step. This is a result of the strong influence of strains which appear in the crystal at the phase separation due to a large difference in molar volume between the phases and probably give rise to plastic deformation of the matrix and to non-equilibrium 3 He concentration in it. The 3 He atom transfer occurs only to the extent of strain relaxation. It is found that the cyclic growth and dissolution of the liquid droplets affect the crystal quality and lead to pressure increase. The coexistence of liquid and solid phases in droplets is speculated to be possible

Quantification of human lung structure and physiology using hyperpolarized 129Xe.

Science.gov (United States)

Chang, Yulin V; Quirk, James D; Ruset, Iulian C; Atkinson, Jeffrey J; Hersman, F William; Woods, Jason C

2014-01-01

To present in vivo, human validation of a previously proposed method to measure key pulmonary parameters related to lung microstructure and physiology. Some parameters, such as blood-air barrier thickness, cannot be measured readily by any other noninvasive modality. Healthy volunteers (n = 12) were studied in 1.5T and 3T whole body human scanners using hyperpolarized xenon. Xenon uptake by lung parenchyma and blood was measured using a chemical shift saturation recovery sequence. Both dissolved-xenon peaks at 197 ppm and 217-218 ppm were fitted against a model of xenon exchange (MOXE) as functions of exchange time. Parameters related to lung function and structure can be obtained by fitting to this model. The following results were obtained from xenon uptake (averaged over all healthy volunteers): surface-area-to-volume ratio = 210 ± 50 cm(-1) ; total septal wall thickness = 9.2 ± 6.5 μm; blood-air barrier thickness = 1.0 ± 0.3 μm; hematocrit = 27 ± 4%; pulmonary capillary blood transit time = 1.3 ± 0.3 s, in good agreement with literature values from invasive experiments. More detailed fitting results are listed in the text. The initial in vivo human results demonstrate that our proposed methods can be used to noninvasively determine lung physiology by simultaneous quantification of a few important pulmonary parameters. This method is highly promising to become a versatile screening method for lung diseases. Copyright © 2013 Wiley Periodicals, Inc.

Carbonyl Reduction of NNK by Recombinant Human Lung Enzymes. Identification of HSD17β12 as the Reductase important in (R)-NNAL formation in Human Lung.

Science.gov (United States)

Ashmore, Joseph H; Luo, Shaman; Watson, Christy J W; Lazarus, Philip

2018-05-17

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is the most abundant and carcinogenic tobacco-specific nitrosamine in tobacco and tobacco smoke. The major metabolic pathway for NNK is carbonyl reduction to form the (R) and (S) enantiomers of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) which, like NNK, is a potent lung carcinogen. The goal of the present study was to characterize NNAL enantiomer formation in human lung and identify the enzymes responsible for this activity. While (S)-NNAL was the major enantiomer of NNAL formed in incubations with NNK in lung cytosolic fractions, (R)-NNAL comprised ~60 and ~95% of the total NNAL formed in lung whole cell lysates and microsomes, respectively. In studies examining the role of individual recombinant reductase enzymes in lung NNAL enantiomer formation, AKR1C1, AKR1C2, AKR1C3, AKR1C4 and CBR1 all exhibited (S)-NNAL formation activity. To identify the microsomal enzymes responsible for (R)-NNAL formation, 28 microsomal reductase enzymes were screened for expression by real-time PCR in normal human lung. HSD17β6, HSD17β12, KDSR, NSDHL, RDH10, RDH11 and SDR16C5 were all expressed at levels >HSD11β1, the only previously reported microsomal reductase enzyme with NNK-reducing activity, with HSD17β12 the most highly expressed. Of these lung-expressing enzymes, only HSD17β12 exhibited activity against NNK, forming primarily (>95%) (R)-NNAL, a pattern consistent with that observed in lung microsomes. siRNA knockdown of HSD17β12 resulted in significant decreases in (R)-NNAL formation activity in HEK293 cells. These data suggest that both cytosolic and microsomal enzymes are active against NNK and that HSD17β12 is the major active microsomal reductase that contributes to (R)-NNAL formation in human lung.

Resolution of Toll-like receptor 4-mediated acute lung injury is linked to eicosanoids and suppressor of cytokine signaling 3.

Science.gov (United States)

Hilberath, Jan N; Carlo, Troy; Pfeffer, Michael A; Croze, Roxanne H; Hastrup, Frantz; Levy, Bruce D

2011-06-01

The purpose of this study was to investigate roles for Toll-like receptor 4 (TLR4) in host responses to sterile tissue injury. Hydrochloric acid was instilled into the left mainstem bronchus of TLR4-defective (both C3H/HeJ and congenic C.C3-Tlr4(Lps-d)/J) and control mice to initiate mild, self-limited acute lung injury (ALI). Outcome measures included respiratory mechanics, barrier integrity, leukocyte accumulation, and levels of select soluble mediators. TLR4-defective mice were more resistant to ALI, with significantly decreased perturbations in lung elastance and resistance, resulting in faster resolution of these parameters [resolution interval (R(i)); ∼6 vs. 12 h]. Vascular permeability changes and oxidative stress were also decreased in injured HeJ mice. These TLR4-defective mice paradoxically displayed increased lung neutrophils [(HeJ) 24×10(3) vs. (control) 13×10(3) cells/bronchoalveolar lavage]. Proresolving mechanisms for TLR4-defective animals included decreased eicosanoid biosynthesis, including cysteinyl leukotrienes (80% mean decrease) that mediated CysLT1 receptor-dependent vascular permeability changes; and induction of lung suppressor of cytokine signaling 3 (SOCS3) expression that decreased TLR4-driven oxidative stress. Together, these findings indicate pivotal roles for TLR4 in promoting sterile ALI and suggest downstream provocative roles for cysteinyl leukotrienes and protective roles for SOCS3 in the intensity and duration of host responses to ALI.

Variations of 3/He/4He isotope ratios within the Broadlands geothermal field, New Zealand

International Nuclear Information System (INIS)

Hulston, John; Lupton, John; University of California, Santa Barbara; Rosenberg, Nina

1986-01-01

The Broadlands-Ohaaki geothermal field is located 20 km NE of Wairakei on the Central Volcanic Zone of New Zealand. It falls within a resistivity low extending 4 km by 3 km. A study of 3 He/ 4 He ratios within this field has shown R/R A ratios close to 6.0 in the Ohaaki production area (NW). In contrast the production area to the SE on the east bank of the Waikato River has R/R A values close to 3.5. Differences in chemical ratios reported previously are found to correlate with the 3 He/ 4 He measurements. A tentative interpretation of the results indicate that there is a contribution of 3 He from the mantle and that the variations in the 3 He/ 4 He ratios are probably related to differences in the geochemistry of the rocks through which the geothermal fluids flow to the surface. Preliminary measurements of the argon isotopes also show a contribution from radiogenic rocks at depth

Angular dependence of the sup(6)Li(πsup(+),sup(3)He)sup(3)He reaction

International Nuclear Information System (INIS)

McParland, B.J.; Auld, E.G.; Couvert, P.

1985-02-01

Angular distributions of the differential cross sections for the pionic fission sup(6)Li(πsup(+),sup(3)He)sup(3)He have been measured at pion energies of 60 and 80 MeV. The differential cross section is found to decrease monotonically with cossup(2)thetasup(*) and is compared with a theoretical prediction

An image acquisition and registration strategy for the fusion of hyperpolarized helium-3 MRI and x-ray CT images of the lung

Science.gov (United States)

Ireland, Rob H.; Woodhouse, Neil; Hoggard, Nigel; Swinscoe, James A.; Foran, Bernadette H.; Hatton, Matthew Q.; Wild, Jim M.

2008-11-01

The purpose of this ethics committee approved prospective study was to evaluate an image acquisition and registration protocol for hyperpolarized helium-3 magnetic resonance imaging (3He-MRI) and x-ray computed tomography. Nine patients with non-small cell lung cancer (NSCLC) gave written informed consent to undergo a free-breathing CT, an inspiration breath-hold CT and a 3D ventilation 3He-MRI in CT position using an elliptical birdcage radiofrequency (RF) body coil. 3He-MRI to CT image fusion was performed using a rigid registration algorithm which was assessed by two observers using anatomical landmarks and a percentage volume overlap coefficient. Registration of 3He-MRI to breath-hold CT was more accurate than to free-breathing CT; overlap 82.9 ± 4.2% versus 59.8 ± 9.0% (p < 0.001) and mean landmark error 0.75 ± 0.24 cm versus 1.25 ± 0.60 cm (p = 0.002). Image registration is significantly improved by using an imaging protocol that enables both 3He-MRI and CT to be acquired with similar breath holds and body position through the use of a birdcage 3He-MRI body RF coil and an inspiration breath-hold CT. Fusion of 3He-MRI to CT may be useful for the assessment of patients with lung diseases.

Accurate 3He polarimetry using the Rb Zeeman frequency shift due to the Rb-3He spin-exchange collisions

International Nuclear Information System (INIS)

Romalis, M.V.; Cates, G.D.

1998-01-01

We describe a method of 3 He polarimetry relying on the polarization-dependent frequency shift of the Rb Zeeman resonance. Our method is ideally suited for on-line measurements of the 3 He polarization produced by spin-exchange optical pumping. To calibrate the frequency shift we performed an accurate measurement of the imaginary part of the Rb- 3 He spin-exchange cross section in the temperature range typical for spin-exchange optical pumping of 3 He. We also present a detailed study of possible systematic errors in the frequency shift polarimetry. copyright 1998 The American Physical Society

Neutron scattering lengths of 3He

International Nuclear Information System (INIS)

Alfimenkov, V.P.; Akopian, G.G.; Wierzbicki, J.; Govorov, A.M.; Pikelner, L.B.; Sharapov, E.I.

1976-01-01

The total neutron scattering cross-section of 3 He has been measured in the neutron energy range from 20 meV to 2 eV. Together with the known value of coherent scattering amplitude it leads to the two sts of n 3 He scattering lengths

X-ray Debye-Waller factor measurements of solid 3He and 4He

International Nuclear Information System (INIS)

Arms, D.A.; Shah, R.S.; Simmons, R.O.

2003-01-01

X-ray synchrotron radiation was used to measure Debye-Waller factors of helium crystals for both 3 He and 4 He in both hcp and fcc phases. To our knowledge, there are no previous measurements for 3 He. The ranges studied for 3 He and 4 He crystals were 11.52-12.82 and 10.95-12.13 cm 3 , respectively, and 11.5-18.2 and 12.0-20.3 K. With small uncertainty, only a Gaussian dependence upon momentum transfer Q was found, and no anisotropy was detected in the hcp phase. Mean square atomic deviations, 2 >, and Lindemann ratios were obtained. Large Lindemann ratios confirm that these solids are highly anharmonic. The 2 > values agree within an average 1% with computations of Draeger and Ceperley from path integral Monte Carlo methods including unusual extrapolations to the thermodynamic limit. Because the path-integral Monte Carlo (PIMC) computations exhibit a T 3 dependence for 2 >, which also depends upon molar volume, an empirical analysis was made of the present data as well as of published x-ray and neutron data on hcp 4 He. The volume dependencies are similar to those found from calorimetry, over a large volume range, and the temperature dependencies show similar systematic variations with molar volume both in x-ray data and PIMC results

Apparent violation of isospin symmetry in the 3H(3He,2H)4He reaction

International Nuclear Information System (INIS)

Rai, G.; Blyth, C.O.; England, J.B.A.; Farooq, A.; Karban, O.; Rawas, E.; Roman, S.; Vlastou, R.

1988-01-01

Angular distributions of the vector analyzing powers for the 3 H( 3 He, 2 H) 4 He reaction have been measured over the incident energy range 18--33 MeV. The measurements centered about 18 MeV display a deviation from the antisymmetric shape expected from isospin symmetry. Concentrating on the explanation of the 90 0 analyzing powers, we report the results of a distorted-wave Born approximation (DWBA) analysis which includes the direct and exchange processes and the spin-orbit potential. It is shown that the anomalous behavior of the 90 0 vector analyzing powers can be largely explained by the effect of a single F-wave potential resonance which leads to the magnification of the short-range differences between the 3 He and 3 H wave functions

12C(3He,3He n)11C cross section at 910 MeV

International Nuclear Information System (INIS)

Aslanides, E.; Fassnacht, P.; Dellacasa, G.; Gallio, M.; Tuyn, J.W.N.

1981-01-01

The 12 C( 3 He, 3 He n) 11 C cross section at 910 MeV was measured by using a Ge(Li) gamma spectrometer to determine the disintegration rate and measuring the incident flux by means of a scintillator telescope. Cross sections for the production of 7 Be in C and Al and for the production of 24 Na in Al are then determined using the measured cross section as a monitor

A case of squamous cell lung cancer after treating with radiation for small cell lung cancer

International Nuclear Information System (INIS)

Hayashi, Toshinari; Ide, Hiroshi; Siomi, Katsuhiko; Nakamura, Yukinobu; Tada, Shinya; Kageyama, Hiroshi; Kido, Masamitsu

1999-01-01

A 77-year-old man was admitted due to an abnormal shadow on a chest X-ray film in September 1993. Small cell lung cancer was diagnosed by transbronchial lung biopsy of left S 3 . Because of his pulmonary and renal dysfunction, he received only 40 Gy irradiation alone, and the tumor shadow disappeared. After 38 months' observation, a new nodular shadow was detected in the left upper lung field in March 1997. A tumor was found in left B 3 by bronchoscopy, and biopsy revealed squamous cell carcinoma. Because of his advanced age and hypoxia, he has had no active treatment. This was a rare case of small cell lung cancer with long term survival, treated only by radiation, in which a different histologic type of carcinoma appeared in the same radiation field. (author)

3He impurities on liquid 4He: possible existence of excited states

International Nuclear Information System (INIS)

Pavloff, N.; Treiner, J.

1989-01-01

We study, using a density functional approach, the properties of the two dimensional system formed by 3 He atoms on the surface of liquid 4 He, as a function of 3 He coverage N s . We find that the excited state recently proposed by Dalfovo and Stringari in the case of infinite dilution does survive when the number of surface 3 He atoms increases. For small values of N s , the surface tension σ is, as expected, linear in N s 2 . For N s ≅0.035 atom per square-Angstrom, i.e. half a monolayer, a new type of surface state starts being occupied, and this feature manifests itself by a change in the slope of σ as a function of N s 2 and, more clearly, by a discontinuity in the surface specific heat, which increases by a factor of almost 2. These predictions call for experimental investigations

Primary lung abscess caused by Staphylococcus lugdunensis.

Science.gov (United States)

Chou, Deng-Wei; Lee, Chao-Tai

2017-11-01

Staphylococcus lugdunensis, a strain of coagulase-negative staphylococci, is part of the normal flora of human skin but can cause multiple infections at various sites. This microorganism has emerged as a major human pathogen. However, no study has reported primary lung abscess caused by S. lugdunensis. A 54-year-old alcoholic man without relevant past medical history was admitted because of primary lung abscesses. Empirical amoxicillin/clavulanate therapy was initially administered; however, the patient had persistent pleuritic chest pain and fever. He subsequently underwent resection of the lung abscess and removal of exudative pleural effusion on the fourth hospital day. Histopathologic examination confirmed the diagnosis of lung abscess, and colonies of gram-positive bacteria were identified. The culture specimen from the abscess was positive for S. lugdunensis, which was susceptible to amoxicillin/clavulanate, cefazolin, ciprofloxacin, clindamycin, erythromycin, oxacillin, teicoplanin, tetracycline, and vancomycin. Following resection and 3 weeks of amoxicillin/clavulanate therapy, the patient eventually recovered well without relapse. This case report is the first to describe S. lugdunensis as a cause of primary lung abscess; this microorganism should be considered a potential monomicrobial pathogen in primary lung abscess. Copyright © 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

Induction of Apoptotic Effects of Antiproliferative Protein from the Seeds of Borreria hispida on Lung Cancer (A549 and Cervical Cancer (HeLa Cell Lines

Directory of Open Access Journals (Sweden)

S. Rupachandra

2014-01-01

Full Text Available A 35 KDa protein referred to as F3 was purified from the seeds of Borreria hispida by precipitation with 80% ammonium sulphate and gel filtration on Sephadex G-100 column. RP-HPLC analysis of protein fraction (F3 on an analytical C-18 column produced a single peak, detected at 220 nm. F3 showed an apparent molecular weight of 35 KDa by SDS PAGE and MALDI-TOF-MS analyses. Peptide mass fingerprinting analysis of F3 showed the closest homology with the sequence of 1-aminocyclopropane-1-carboxylate deaminase of Pyrococcus horikoshii. The protein (F3 exhibited significant cytotoxic activity against lung (A549 and cervical (HeLa cancer cells in a dose-dependent manner at concentrations ranging from 10 µg to 1000 µg/mL, as revealed by the MTT assay. Cell cycle analysis revealed the increased growth of sub-G0 population in both cell lines exposed to a concentration of 1000 µg/mL of protein fraction F3 as examined from flow cytometry. This is the first report of a protein from the seeds of Borreria hispida with antiproliferative and apoptotic activity in lung (A549 and cervical (HeLa cancer cells.

Radiogenic 3He/4He Estimates and Their Effect on Calculating Plio-Pleistocene Cosmogenic 3He Ages of Alluvial-Fan Terraces in the Lower Colorado River Basin, USA

Science.gov (United States)

Fenton, C.; Pelletier, J.

2005-12-01

Several alluvial-fan terraces near Topock, AZ were created by successive entrenchment of Pliocene and Pleistocene alluvial-fan gravels shed from the adjacent Black Mountains along the lower Colorado River corridor below Hoover Dam. These fans interfinger with and overlie main-stem Colorado River sands and gravels and grade to terrace levels that correspond with pre-existing elevations of the Colorado River. Absolute dates for the ages of Quaternary deposits on the lower Colorado River are rare and cosmogenic 3He age estimates of these surfaces would help constrain the timing of aggradation and incision in the lower Colorado River corridor. We analyzed individual basalt boulders from several terrace surfaces for total 3He/4He concentrations to calculate cosmogenic 3He ages of each fan terrace; 3He/4He values, expressed as R/Ra where Ra is the 3He/4He of air, range from 0.29 to 590. Black Mountain volcanic rocks have reported K-Ar ages between 15 and 30 Ma and basalt samples from adjacent alluvial fans contain 0.42 to 47× 1012 at/g of 4He, which has likely accumulated due to nuclear processes. The amount of radiogenic 3He/4He can be significant in old rocks with young exposure ages and can complicate determination of cosmogenic 3 He content. Alpha-decay of U, Th, and their daughter isotopes produces large amounts of 4He, whereas significant amounts of radiogenic 3He are only produced through the neutron bombardment of Li and subsequent beta-decay of tritium. We measured Li, U, Th, major and rare-earth element concentrations in whole-rock basalts and mineral separates. These concentrations are used to estimate the ratio of radiogenic helium contributed to the total helium system in our samples. Li concentrations typically range from 6 to 17 ppm, with one outlier of 62 ppm. U contents range from calculations predict that the average radiogenic helium (R/Ra) contributed to the total helium in Black Mountain basalt samples is 0.011. Other noble gas studies have shown

Radiation-Induced Differentiation in Human Lung Fibroblast

International Nuclear Information System (INIS)

Park, Sa-Rah; Ahn, Ji-Yeon; Han, Young-Soo; Shim, Jie-Young; Yun, Yeon-Sook; Song, Jie-Young

2007-01-01

One of the most common tumors in many countries is lung cancer and patients with lung cancer may take radiotherapy. Although radiotherapy may have its own advantages, it can also induce serious problems such as acute radiation pneumonitis and pulmonary fibrosis. Pulmonary fibrosis is characterized by excessive production of α-SMA and accumulation of extracellular matrix (ECM) such as collagen and fibronectin. There has been a great amount of research about fibrosis but the exact mechanism causing the reaction is not elucidated especially in radiation-induced fibrosis. Until now it has been known that several factors such as transforming growth factor (TGF-β), tumor necrosis factor (TNF), interleukin (IL)-1, IL-6, platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) are related to fibrosis. Among them TGF-β with Smad signaling is known to be the main stream and other signaling molecules such as MAPK, ERK and JNK (3) also participates in the process. In addition to those above factors, it is thought that more diverse and complicate mechanisms may involve in the radiationinduced fibrosis. Therefore, to investigate the underlying mechanisms in radiation induced fibrosis, first of all, we confirmed whether radiation induces trans differentiation in human normal lung fibroblasts. Here, we suggest that not only TGF-β but also radiation can induce trans differentiation in human lung fibroblast WI-38 and IMR-90

Temperature dependence of He(2 3PJ) reactions: Collision-induced mixing and conversion to He2( 3Πg) molecules

International Nuclear Information System (INIS)

Zhao, X.; Soletsky, P.A.; Bryan, W.H.; Dunning, F.B.; Walters, G.K.

1993-01-01

The rate coefficients for mixing between He(2 3 P J, MJ) levels during collisions with ground-state helium atoms and for conversion of He(2 3 P J ) atoms to He 2 (b 3 Π g ) molecules via three-body reactions in helium gas have been investigated over the temperature range 1.6--300 K. The measured rate coefficients for collisionally induced P-state mixing decrease slowly with decreasing temperature, from (1.8±0.5)x10 -9 cm 3 s -1 at 300 K to (4.5±0.5)x10 -10 cm 3 s -1 at 4.2 K. The rate coefficients for the production of He 2 (b 3 Π g ) molecules via three-body reactions are observed to increase with decreasing temperature and are described by the relation k P congruent(2.5+267T -1 )x10 -32 cm 6 s -1 . This behavior, which is very different from that noted in earlier studies of the conversion of He(2 3 S 1 ) atoms to He 2 (a 3 Σ u + ) molecules through three-body reactions, suggests that the reaction is not thermally activated

Neurotrophins expression is decreased in lungs of human infants with congenital diaphragmatic hernia

Directory of Open Access Journals (Sweden)

O'Hanlon LD

2014-02-01

Full Text Available Lynn D O'Hanlon, Sherry M Mabry, Ikechukwu I EkekezieChildren's Mercy Hospitals/University of Missouri-Kansas City School of Medicine, Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Kansas City, MO, USAObjectives: To evaluate neurotrophin (NT (nerve growth factor [NGF], NT-3, and brain-derived neurotrophic factor [BDNF] expression in autopsy lung tissues of human congenital diaphragmatic hernia (CDH infants versus that of infants that expired with: 1 "normal" lungs (controls; 2 chronic lung disease (CLD; and 3 pulmonary hypertension (PPHN.Hypothesis: NT expression will be significantly altered in CDH lung tissue compared with normal lung tissue and other neonatal lung diseases.Study design: Immunohistochemical studies for NT proteins NGF, BDNF, and NT-3 were applied to human autopsy neonatal lung tissue samples.Subject selection: The samples included a control group of 18 samples ranging from 23-week gestational age to term, a CDH group of 15 samples, a PPHN group of six samples, and a CLD group of 12 samples.Methodology: The tissue samples were studied, and four representative slide fields of alveoli/saccules and four of bronchioles were recorded from each sample. These slide fields were then graded (from 0 to 3 by three blinded observers for intensity of staining.Results: BDNF, NGF, and NT-3 immunostaining intensity scores were significantly decreased in the CDH lung tissue (n=15 compared with normal neonatal lung tissue (n=18 (P<0.001. The other neonatal pulmonary diseases that were studied, CLD and PPHN, were much less likely to be affected and were much more variable in their neurotrophin expression.Conclusion: NT expression is decreased in CDH lungs. The decreased expression of NT in CDH lung tissue may suggest they contribute to the abnormality in this condition.Keywords: nerve growth factor, NGF, brain-derived neurotrophic factor, BDNF, neurotrophin-3, NT-3, chronic lung disease, persistent pulmonary hypertension, lung

Human SUV3 helicase regulates growth rate of the HeLa cells and can localize in the nucleoli.

Science.gov (United States)

Szewczyk, Maciej; Fedoryszak-Kuśka, Natalia; Tkaczuk, Katarzyna; Dobrucki, Jurek; Waligórska, Agnieszka; Stępień, Piotr P

2017-01-01

The human SUV3 helicase (SUV3, hSUV3, SUPV3L1) is a DNA/RNA unwinding enzyme belonging to the class of DexH-box helicases. It localizes predominantly in the mitochondria, where it forms an RNA-degrading complex called mitochondrial degradosome with exonuclease PNP (polynucleotide phosphorylase). Association of this complex with the polyA polymerase can modulate mitochondrial polyA tails. Silencing of the SUV3 gene was shown to inhibit the cell cycle and to induce apoptosis in human cell lines. However, since small amounts of the SUV3 helicase were found in the cell nuclei, it was not clear whether the observed phenotypes of SUV3 depletion were of mitochondrial or nuclear origin. In order to answer this question we have designed gene constructs able to inhibit the SUV3 activity exclusively in the cell nuclei. The results indicate that the observed growth rate impairment upon SUV3 depletion is due to its nuclear function(s). Unexpectedly, overexpression of the nuclear-targeted wild-type copies of the SUV3 gene resulted in a higher growth rate. In addition, we demonstrate that the SUV3 helicase can be found in the HeLa cell nucleoli, but it is not detectable in the DNA-repair foci. Our results indicate that the nucleolar-associated human SUV3 protein is an important factor in regulation of the cell cycle.

Homogeneous nucleation in phase separation of solid 3He-4He mixtures

International Nuclear Information System (INIS)

Poole, M.; Smith, A.; Maidanov, V.A.; Rudavskii, E.Ya.; Grigor'ev, V.N.; Slezov, V.V.; Saunders, J.; Cowan, B.

2003-01-01

NMR and pressure have been measured during phase separation in solid 3 He- 4 He mixtures. Spin echoes were used to observe bounded diffusion and to estimate the diffusion coefficient, size and nuclei concentration in the 3 He-enriched phase. The characteristic phase separation time constant of the mixture was found from pressure measurements. The results argue convincingly for homogeneous nucleation. The surface tension of the nuclei is found independently from NMR and from pressure measurements; the two determinations agree well and yield a surface tension coefficient of 4.9x10 -6 J m -2

Spin effects in medium-energy electron-3He scattering

International Nuclear Information System (INIS)

van den Brand, J.F.J.; Alarcon, R.; Bauer, T.

1998-01-01

New physics can be accessed by scattering polarized electrons from a polarized 3 He internal gas target. It is discussed how the asymmetries for the reactions 3 vector He(vector e,e'), 3 vector He(vector e,e'p), 3 vector He(vector e,e'n), 3 vector He(vector e,e'd), and 3 vector He(vector e,e'pn) may provide precise information on the S' and the D-wave parts of the 3 He ground-state wave function, the neutron form factors, and the role of spin-dependent reaction mechanism effects. The experiment uses up to 900 MeV (polarized) electrons from the AmPS storage ring in Amsterdam, Netherlands, in combination with large acceptance electron and hadron detectors. (orig.)

Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene

DEFF Research Database (Denmark)

Stoner, G.D.; Harris, C.C.; Autrup, Herman

1978-01-01

the predominant alveolar epithelial cell type. Lamellar inclusion bodies were released from the type 2 cells and accumulated in the alveolar spaces. The metabolism of benzo[alpha]pyrene (BP) in human lung explants cultured for up to 7 days was investigated. Human lung explants had measurable aryl hydrocarbon......Human lung explants have been maintained in vitro for a period of 25 days. Autoradiographic studies indicated that the broncholar epithelial cells, type 2 alveolar epithelial cells, and stromal fibroblasts incorporated 3H-thymidine during the culture. After 7 to 10 days, type 2 cells were...... hydroxylase activity and could metabolize BP into forms that were bound to cellular DNA and protein. Peripheral lung had significantly lower aryl hydrocarbon hydroxylase activity than cultured bronchus but both tissues had similar binding levels of BP to DNA. Radioautographic studies indicated that all cell...

The 3H–3He Charge Radii Difference

Directory of Open Access Journals (Sweden)

Myers L. S.

2016-01-01

Full Text Available The upcoming E12-14-009 [1] experiment at Jefferson Lab will determine the ratio of the electric form factors for the A=3 mirror nuclei 3He and 3H. The measurement will use a 1.1 GeV electron beam, a special collimator plate to allow for simultaneous optics measurements, and the low-activity tritium target being prepared for Jefferson Lab. By observing the dependence of the form factor ratio as a function of Q2 over 0.05–0.09 GeV2, the dependence of the radii extraction on the shape of the form factors is minimized. As a result, we anticipate the uncertainty of the extracted charge radii difference to be 0.03 fm, a reduction of 70% from the current measurement. Using precise measurements of the 3He charge radius from isotopic shift or μHe measurements [2–4], we can deduce the absolute 3H charge radius. The results will provide a direct comparison to recent calculations of the charge radii.

99Tcm-N(NOEt2 Uptake Kinetics Difference among KMB17 Human Embryonic Lung Diploid Fibroblast and Different Human Lung Cancer Cells

Directory of Open Access Journals (Sweden)

Wei JIA

2010-04-01

Full Text Available Background and objective PET/CT imaging is expensive, so searching the tumor imaging agent for SPECT/CT is necessary. 99Tcm-N(NOEt2 [bis (N-ethoxy-N-ethyl dithiocarbamato nitrido99Tcm (V] can be uptaken by lung cancer cells and other cells alike. The aim of this study is to evaluate the distinctive value in lung tumor with 99Tcm-N(NOEt2, the difference in its uptake kinetics in human embryonic lung diploid fibroblasts KMB17 and several kinds of lung cancer cells lines. Methods Firstly, six different cell culture medium which contained YTMLC Gejiu human lung squamous carcinoma cell, SPC-A1 human lung adenocarcinoma cell, AGZY low metastatic human lung adenocarcinoma, 973 high metastatic human lung adenocarcinoma cell, GLC-82 Gejiu human lung adenocarcinoma cell, and KMB17 human embryonic lung diploid fibroblast, respectively with equal cell density of 1×106/mL and the same volume were prepared; secondly, the same radioactive dose of 99Tcm-N(NOEt2 was added into each sample and then 300 μL mixed sample was taken out respectively and cultured in 37 oC culture box; Finally, 5 min, 15 min, 30 min, 45 min, 60 min, 75 min, 90 min after cultivation, centrifuged each cultured sample and determined the intracellular radiocounts of each sample, calculated each cell sample’s uptake rate of 99Tcm-N(NOEt2 at different time. Results Statistical difference was found among six cell samples, and the uptake rate sequence from high to low is 973 and SPC-A1>YTMLC>GLC-82>AGZY>KMB17 respectively; furthermore, 30 min-45 min after culture, the uptake rate reached stability, and the 45 min uptake rate of each sample was higher than its 96.7% uptake peak. Conclusion Based on the results above mentioned, it is supposed that there are discriminative clinical value when using 99Tcm-N(NOEt2 as a tumor targeting imaging agent, and 30 min or so after injection may be the best imaging time in the early imaging stage.

Nonlinear second- and first-sound wave equations in 3He-4He mixtures

International Nuclear Information System (INIS)

Mohazzab, Masoud; Mulders, Norbert

2000-01-01

We derive nonlinear Burgers equations for first and second sound in mixtures of 3 He- 4 He, using a reductive perturbation method and obtain expressions for the nonlinear and dissipation coefficients. We further find a diffusion equation for a coupled temperature-concentration mode. The amplitude of first (second) sound generated from second (first) sound in mixtures is also derived. Our derivation includes the dependence of thermodynamical quantities on temperature, pressure, and 3 He concentration, and is valid up to a first order in terms of the isobaric expansion coefficient. We show that close to the λ line the nonlinearity of second sound in mixtures is enhanced as compared with pure 4 He

Study of the 6Li + p → 3He + 4He reaction in inverse kinematics

International Nuclear Information System (INIS)

Betsou, C.; Pakou, A.; Aslanoglou, X.; Nicolis, N.G.; Sgouros, O.; Soukeras, V.; Cappuzzello, F.; Acosta, L.; Agodi, C.; Carbone, D.; Cavallaro, M.; Di Pietro, A.; Fernandez-Garcia, J.P.; Figuera, P.; Fisichella, M.; Foti, A.; Keeley, N.; Marquinez-Duran, G.; Martel, I.; Mazzocco, M.; Strano, E.; Torresi, D.; Pierroutsakou, D.; Rusek, K.; Stiliaris, E.

2015-01-01

Angular distribution measurements were performed for the 6 Li + p → 3 He + 4 He reaction in inverse kinematics at incident energies of 2.7, 3.3, 4.2 and 4.8 MeV/u. The detection of both recoils ( 3 He and 4 He) over the laboratory angle range θ lab = 16 circle to 34 circle allowed the determination of the angular distribution over a wide angular range in the center-of-mass frame (θ c.m. ∝ 40 circle to 140 circle ). The results clarify inconsistencies between existing data sets and are consistent with compound nucleus model calculations. (orig.)

Opportunities for Polarized He-3 in RHIC and EIC

Energy Technology Data Exchange (ETDEWEB)

Aschenauer E.; Deshpande, A.; Fischer, W.; Derbenev, S.; Milner, R.; Roser, T.; Zelenski, A.

2011-10-01

The workshop on opportunities for polarized He-3 in RHIC and EIC was targeted at finding practical ways of implementing and using polarized He-3 beams. Polarized He-3 beams will provide the unique opportunity for first measurements, i.e, to a full quark flavor separation measuring single spin asymmetries for p{sup +}, p{sup -} and p{sup 0} in hadron-hadron collisions. In electron ion collisions the combination of data recorded with polarized electron proton/He-3 beams allows to determine the quark flavor separated helicity and transverse momentum distributions. The workshop had sessions on polarized He-3 sources, the physics of colliding polarized He-3 beams, polarimetry, and beam acceleration in the AGS Booster, AGS, RHIC, and ELIC. The material presented at the workshop will allow making plans for the implementation of polarized He-3 beams in RHIC.

Expression of PFKFB3 and Ki67 in lung adenocarcinomas and targeting PFKFB3 as a therapeutic strategy.

Science.gov (United States)

Li, Xiaoli; Liu, Jian; Qian, Li; Ke, Honggang; Yao, Chan; Tian, Wei; Liu, Yifei; Zhang, Jianguo

2018-01-11

Phosphofructokinase-2/fructose-2, 6-bisphosphatase 3 (PFKFB3) catalyzes the synthesis of F2,6BP, which is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1): the rate-limiting enzyme of glycolysis. During tumorigenesis, PFKFB3 increases glycolysis, angiogenesis, and tumor progression. In this study, our aim was to investigate the significance of PFKFB3 and Ki67 in human lung adenocarcinomas and to target PFKFB3 as a therapeutic strategy. In this study, we determined the expression levels of PFKFB3 mRNA and proteins in cancerous and normal lung adenocarcinomas by quantitative reverse transcription PCR (qRT-PCR), Western blot analysis, and tissue microarray immunohistochemistry analysis, respectively. In human adenocarcinoma tissues, PFKFB3 and Ki67 protein levels were related to the clinical characteristics and overall survival. Both PFKFB3 mRNA and protein were significantly higher in lung adenocarcinoma cells (all P targeting PFKFB3, it inhibited cell viability and glycolytic activity. It also caused apoptosis and induced cell cycle arrest. Furthermore, the migration and invasion of A549 cells was inhibited. We conclude that PFKFB3 bears an oncogene-like regulatory element in lung adenocarcinoma progression. In the treatment of lung adenocarcinoma, targeting PFKFB3 would be a promising therapeutic strategy.

Backward elastic p3He-scattering and high momentum components of 3He wave function

International Nuclear Information System (INIS)

Uzikov, Yu.N.

1998-01-01

It is shown that owing to a dominance of np-pair transfer mechanism of backward elastic p 3 He-scattering for incident proton kinetic energies T p > 1 GeV the cross section of this process is defined mainly by the values of the Faddeev component of the wave function of 3 He nucleus, φ 23 (q 23 , p 1 ), at high relative momenta q 23 > 0.6 GeV/c of the NN-pair in the 1 S 0 -state and at low spectator momenta p 1 ∼ 0 - 0.2 GeV/c

Electric dipole moment of 3He

International Nuclear Information System (INIS)

Avishai, Y.; Fabre de la Ripelle, M.

1987-01-01

The contribution of CP violating nucleon-nucleon interaction to the electric dipole moment of 3 He is evaluated following a recent proposal for its experimental detection. Two models of CP violating interactions are used, namely, the Kobayashi-Maskawa mechanism and the occurrence of the Θ term in the QCD lagrangian. These CP violating interactions are combined with realistic strong nucleon-nucleon interactions to induce a CP forbidden component of the 3 He wave function. The matrix element of the electric dipole operator is then evaluated between CP allowed and CP forbidden components yielding the observable electric dipole moment. Using the parameters emerging from the penguin terms in the Kobaysashi-Maskawa model we obtain a result much larger than the electric dipole moment of the neutron in the same model. On the other hand, no enhancement is found for the Θ-term mechanism. A possible explanation for this difference is discussed. Numerical estimates can be given only in the Kobayashi-Maskawa model, giving d( 3 He) ≅ 10 30 e . cm. In the second mechanism, the estimate give d ( 3 He) ≅ 10 16 anti Θ. (orig.)

Superfluidity in two-dimensions. A thermal conductivity study of 4He and 3He- 4He mixture films

International Nuclear Information System (INIS)

Finotello, D.

1992-01-01

We review measurement of the thermal transport of 4 He films and 3 He- 4 He mixture films near the superfluid transition. These measurements were performed on helium films of thickness ranging from 12 to 156 A and mixture films with 3 He concentration up to 2%. The superfluid transition temperature for these films ranged from 1.2 to 2.2. K. We discuss universal features of the data as well as the behavior of the ratio of the vortex diffusion constant to vortex core-parameter, the sharpness of the transition and the superfluid transition temperature as a function of thickness and concentration. We also describe new experiments that will contribute to a better understanding of the observed behavior (Author)

Human lung fibroblast-derived matrix facilitates vascular morphogenesis in 3D environment and enhances skin wound healing.

Science.gov (United States)

Du, Ping; Suhaeri, Muhammad; Ha, Sang Su; Oh, Seung Ja; Kim, Sang-Heon; Park, Kwideok

2017-05-01

Extracellular matrix (ECM) is crucial to many aspects of vascular morphogenesis and maintenance of vasculature function. Currently the recapitulation of angiogenic ECM microenvironment is still challenging, due mainly to its diverse components and complex organization. Here we investigate the angiogenic potential of human lung fibroblast-derived matrix (hFDM) in creating a three-dimensional (3D) vascular construct. hFDM was obtained via decellularization of in vitro cultured human lung fibroblasts and analyzed via immunofluorescence staining and ELISA, which detect multiple ECM macromolecules and angiogenic growth factors (GFs). Human umbilical vein endothelial cells (HUVECs) morphology was more elongated and better proliferative on hFDM than on gelatin-coated substrate. To prepare 3D construct, hFDM is collected, quantitatively analyzed, and incorporated in collagen hydrogel (Col) with HUVECs. Capillary-like structure (CLS) formation at 7day was significantly better with the groups containing higher doses of hFDM compared to the Col group (control). Moreover, the group (Col/hFDM/GFs) with both hFDM and angiogenic GFs (VEGF, bFGF, SDF-1) showed the synergistic activity on CLS formation and found much larger capillary lumen diameters with time. Further analysis of hFDM via angiogenesis antibody array kit reveals abundant biochemical cues, such as angiogenesis-related cytokines, GFs, and proteolytic enzymes. Significantly up-regulated expression of VE-cadherin and ECM-specific integrin subunits was also noticed in Col/hFDM/GFs. In addition, transplantation of Col/hFMD/GFs with HUVECs in skin wound model presents more effective re-epithelialization, many regenerated hair follicles, better transplanted cells viability, and advanced neovascularization. We believe that current system is a very promising platform for 3D vasculature construction in vitro and for cell delivery toward therapeutic applications in vivo. Functional 3D vasculature construction in vitro is still

Specific features of kinetics of He3-He4 solid solution transformations at superlow temperatures

International Nuclear Information System (INIS)

Mikheev, V.A.; Majdanov, V.A.; Mikhin, N.P.

1986-01-01

The NMR data on the phase transition kinetics of 3 He- 4 He solid solutions at T=100 mK are considered. Studied are solid helium samples of a molecular volume of 20.55 cm 2 /mol with a 3 He content of 0.54 %. An unusually long phase transition time is found which is dependent on the prehistory of sample. The spin diffusion of 3 He in the transformated solution concentrated phase is found to be of a quasi-one-dimensional nature with the diffusion coefficient value typical of liquid

The environmental carcinogen 3-nitrobenzanthrone and its main metabolite 3-aminobenzanthrone enhance formation of reactive oxygen intermediates in human A549 lung epithelial cells

International Nuclear Information System (INIS)

Hansen, Tanja; Seidel, Albrecht; Borlak, Juergen

2007-01-01

The environmental contaminant 3-nitrobenzanthrone (3-NBA) is highly mutagenic and a suspected human carcinogen. We aimed to evaluate whether 3-NBA is able to deregulate critical steps in cell cycle control and apoptosis in human lung epithelial A549 cells. Increased intracellular Ca 2+ and caspase activities were detected upon 3-NBA exposure. As shown by cell cycle analysis, an increased number of S-phase cells was observed after 24 h of treatment with 3-NBA. Furthermore, 3-NBA was shown to inhibit cell proliferation when added to subconfluent cell cultures. The main metabolite of 3-NBA, 3-ABA, induced statistically significant increases in tail moment as judged by alkaline comet assay. The potential of 3-NBA and 3-ABA to enhance the production of reactive oxygen species (ROS) was demonstrated by flow cytometry using 2',7'-dichlorofluorescein-diacetate (DCFH-DA). The enzyme inhibitors allopurinol, dicumarol, resveratrol and SKF525A were used to assess the impact of metabolic conversion on 3-NBA-mediated ROS production. Resveratrol decreased dichlorofluorescein (DCF) fluorescence by 50%, suggesting a role for CYP1A1 in 3-NBA-mediated ROS production. Mitochondrial ROS production was significantly attenuated (20% reduction) by addition of rotenone (complex I inhibition) and thenoyltrifluoroacetone (TTFA, complex II inhibition). Taken together, the results of the present study provide evidence for a genotoxic potential of 3-ABA in human epithelial lung cells. Moreover, both compounds lead to increased intracellular ROS and create an environment favorable to DNA damage and the promotion of cancer

Specific features of concentrated phase under decomposition of weak solid /sup 3/He-/sup 4/He solution

Energy Technology Data Exchange (ETDEWEB)

Mikheev, V A; Majdamov, V A; Kal' noj, S E; Omelaenko, N I

1988-06-01

The decomposition of solid /sup 3/He-/sup 4/He solutuions is studied on the samples 0.54% /sup 3/He(V=20.55 cm/sup 3//mole) and 0.60% /sup 4/He (V=24.04-24.93 cm/sup 3//mole) using pulse NMR method. At T=100 mK the decomposition of a weak solution proceeds more than for 30 h, the decomposition rate and temperature being dependent on the sample prehistory. In the concentrated phase of the decomposed weak solution the spin diffraction of /sup 3/He is of the quasi-one-dimensional character with the diffusion coefficient D /similar to/ 10/sup -5/ cm/sup 2//sec typical of liquid /sup 3/He and exceeding that bulk solid /sup 3/He by two orders of magnitude. The longitudinal relaxation time in the quasi-one-dimensional phase (/similar to/ 1 sec) is characteristic of the solid state and coinsides with data for bulk /sup 3/He. The temperature behaviour of magnetization in the quasi-one-dimensional phase is well described by the Curie law.

Spin polarized 3He: a ''new'' quantum fluid

International Nuclear Information System (INIS)

Lhuillier, C.; Laloe, F.

1979-01-01

The physical properties of a 3 He fluid are studied, in which all nuclear spins are parallel to each other (fully polarized 3 He). At low temperatures, significant differences can exist between this polarized fluid and normal 3 He. The origin of these differences is purely quantum mechanical and arises from the Pauli exclusion principle. At low densities, only the transport properties of the gas are modified. At higher densities. The equilibrium properties (virial coefficients) are also changed by the nuclear polarization. Changes of the liquid-vapour or liquid-solid equilibrium pressures, as well as modifications of the 3 He- 4 He mixture phase diagram are predicted. This article gives a preliminary theoretical study of these new effects. Experimental prospects are briefly discussed [fr

Three-critical phenomena in the He3-He4 mixture

International Nuclear Information System (INIS)

Carvalho Filho, C.A.A. de.

1976-07-01

A review of the phenomenology of tricritical behaviour in He 3 -He 4 mixtures is made. Starting from a model proposed by Blume, Emery and Griffiths the tricritical transition is investigated by means of the Renormalization Group making use of the Callan-Symanzik equation and usual perturbative methods of Relativistic Quantum Field Theory. Mean-field tricritical exponents and logarithmic corrections are found in a sup(n) unified context. The structure of scaling laws is also studied. (author) [pt

3He functions in tokamak-pumped laser systems

International Nuclear Information System (INIS)

Jassby, D.L.

1986-10-01

3 He placed in an annular cell around a tokamak fusion generator can convert moderated fusion neutrons to energetic ions by the 3 He(n,p)T reaction, and thereby excite gaseous lasants mixed with the 3 He while simultaneously breeding tritium. The total 3 He inventory is about 4 kg for large tokamak devices. Special configurations of toroidal-field magnets, neutron moderators and beryllium reflectors are required to permit nearly uniform neutron current into the laser cell with minimal attenuation. The annular laser radiation can be combined into a single output beam at the top of the tokamak

Thermal transport properties in dilute 3He-4He mixtures near the superfluid transition

International Nuclear Information System (INIS)

Tuttle, J.G.

1991-01-01

The author describes measurements of various transport properties of dilute liquid 3 He- 4 He mixtures in two different sample cells. One cell was designed to measure the temperature drop ΔT across a fluid layer due to an applied heat flux Q, and the other allowed measurement of both ΔT and ΔX, the molar concentration drop. He studied the anomalous boundary resistance, ΔR b , in superfluid 4 He near T λ for Q between 4 and 100μW/cm 2 . He presents results for both the weakly divergent and the heat dependent, more strongly divergent contributions. He compares his data with those of Duncan and Ahlers and with theoretical predictions by Frank and Dohm. He also presents similar R b data for a mixture with X = 2.5 x 10 -6 . He determined the relaxation time τ for transients observed during thermal conductivity κ eff and thermal diffusion k T * measurements in superfluid mixtures between 1.7K and T λ with X ranging from 10 -6 to 5 x 10 -2 . He compares these results, taken with a fluid layer thickness h = 1.81 mm, with theoretical predictions and with earlier data for h = 1.47 mm. He shows the mass diffusion coefficient D computed both from τ and from κ eff data for 0.001 λ , and he compares the results of these two different determinations. In addition, he presents the 3 He-roton scattering cross section calculated using D results. In the normal phase he measured the thermal diffusion ratio k T and the thermal conductivity κ for 0.009 ≤ X ≤ 0.05 near T λ , and he compares these results with predictions by Dohm and Folk

CYP3A-mediated apoptosis of dauricine in cultured human bronchial epithelial cells and in lungs of CD-1 mice

International Nuclear Information System (INIS)

Jin, Hua; Shen, Shuijie; Chen, Xiaoyan; Zhong, Dafang; Zheng, Jiang

2012-01-01

Dauricine is the major bioactive component isolated from the root of Menispermum dauricum DC and has shown promising pharmacologic activities with a great potential for clinical use. Recently, we found that intraperitoneal exposure of dauricine produced selective pulmonary injury in mice. A quinone methide metabolite of dauricine was identified and is suggested to be associated with the pulmonary toxicity of dauricine. The present study evaluated the apoptotic effect of dauricine in cultured cells and mice, determined the change in cellular glutathione (GSH) contents after exposure to dauricine, investigated the role of GSH depletion in dauricine-induced cytotoxicity and apoptosis, and examined the role of CYP3A in dauricine-induced GSH depletion and apoptosis. Dauricine was found to induce apoptosis in NL-20 cells. Additionally, intraperitoneal administration of dauricine caused GSH depletion and apoptosis in lungs of mice. Treatment with ketoconazole, an inhibitor of CYP3A, reversed cellular GSH depletion in lungs of mice given dauricine and showed protective effect on dauricine-induced apoptosis in lungs of mice. This indicates that metabolic activation is involved in dauricine-induced GSH-depletion, cytotoxicity and apoptosis. The glutathione depletor L-buthionine sulfoximine showed potentiating effect on cytotoxicity and apoptosis induced by dauricine. We propose that dauricine is metabolized to a quinone methide intermediate which depletes cellular GSH, and the depletion of GSH may trigger and/or intensify the cytotoxicity and apoptosis induced by dauricine. -- Highlights: ► Dauricine induced apoptosis in lungs in mice and in cultured human pulmonary cells. ► Dauricine depleted cellular GSH in lungs of mice and in the human pulmonary cells. ► CYP3A subfamily mediated GSH depletion and apoptosis induced by dauricine. ► L-Buthionine sulfoximine potentiated dauricine-induced GSH depletion and apoptosis.

CYP3A-mediated apoptosis of dauricine in cultured human bronchial epithelial cells and in lungs of CD-1 mice

Energy Technology Data Exchange (ETDEWEB)

Jin, Hua; Shen, Shuijie [Center for Developmental Therapeutics, Seattle Children' s Research Institute, Division of Gastroenterology and Hepatology, Department of Pediatrics, University of Washington, Seattle, WA 98101 (United States); Chen, Xiaoyan; Zhong, Dafang [Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203 (China); Zheng, Jiang, E-mail: jiang.zheng@seattlechildrens.org [Center for Developmental Therapeutics, Seattle Children' s Research Institute, Division of Gastroenterology and Hepatology, Department of Pediatrics, University of Washington, Seattle, WA 98101 (United States)

2012-06-15

Dauricine is the major bioactive component isolated from the root of Menispermum dauricum DC and has shown promising pharmacologic activities with a great potential for clinical use. Recently, we found that intraperitoneal exposure of dauricine produced selective pulmonary injury in mice. A quinone methide metabolite of dauricine was identified and is suggested to be associated with the pulmonary toxicity of dauricine. The present study evaluated the apoptotic effect of dauricine in cultured cells and mice, determined the change in cellular glutathione (GSH) contents after exposure to dauricine, investigated the role of GSH depletion in dauricine-induced cytotoxicity and apoptosis, and examined the role of CYP3A in dauricine-induced GSH depletion and apoptosis. Dauricine was found to induce apoptosis in NL-20 cells. Additionally, intraperitoneal administration of dauricine caused GSH depletion and apoptosis in lungs of mice. Treatment with ketoconazole, an inhibitor of CYP3A, reversed cellular GSH depletion in lungs of mice given dauricine and showed protective effect on dauricine-induced apoptosis in lungs of mice. This indicates that metabolic activation is involved in dauricine-induced GSH-depletion, cytotoxicity and apoptosis. The glutathione depletor L-buthionine sulfoximine showed potentiating effect on cytotoxicity and apoptosis induced by dauricine. We propose that dauricine is metabolized to a quinone methide intermediate which depletes cellular GSH, and the depletion of GSH may trigger and/or intensify the cytotoxicity and apoptosis induced by dauricine. -- Highlights: ► Dauricine induced apoptosis in lungs in mice and in cultured human pulmonary cells. ► Dauricine depleted cellular GSH in lungs of mice and in the human pulmonary cells. ► CYP3A subfamily mediated GSH depletion and apoptosis induced by dauricine. ► L-Buthionine sulfoximine potentiated dauricine-induced GSH depletion and apoptosis.

Experimental Study of Photon Induced Reactions on 3He and 4He at Low Energies

International Nuclear Information System (INIS)

Tornow, W.

2011-01-01

Data are reported for the photodisintegration cross section of the reaction 3 He(γ, p) 2 H at ten energies between 7.0 and 16.0 MeV. Very preliminary data are presented for the reaction 4 He(γ, p) 3 H between 22.0 and 29.5 MeV in 0.5 MeV energy steps, and for the reaction 4 He(γ, n) 3 He at three energies around 28.0 MeV. High-pressure He/Xe gas scintillators served as target and detector. Our data are in better agreement with recent theoretical calculations than the majority of the existing data for all three reactions, but differ significantly from recent data taken with a mono-energetic photon beam and a time-projection chamber. (author)

Neutron polarization in polarized 3He targets

International Nuclear Information System (INIS)

Friar, J.L.; Gibson, B.F.; Payne, G.L.; Bernstein, A.M.; Chupp, T.E.

1990-01-01

Simple formulas for the neutron and proton polarizations in polarized 3 He targets are derived assuming (1) quasielastic final states; (2) no final-state interactions; (3) no meson-exchange currents; (4) large momentum transfers; (5) factorizability of 3 He SU(4) response-function components. Numerical results from a wide variety of bound-state solutions of the Faddeev equations are presented. It is found that this simple model predicts the polarization of neutrons in a fully polarized 3 He target to be 87%, while protons should have a slight residual polarization of -2.7%. Numerical studies show that this model works very well for quasielastic electron scattering

The osmotic pressure of 3He-4He mixtures along the phase separation curve

NARCIS (Netherlands)

van de Klundert, L.J.M.; Bos, M.R.E.; van der Meij, J.A.M.; Steffens, H.A.

1977-01-01

The osmotic pressure of 3He-4He mixtures was measured along the phase separation curve at temperatures up to 500 mK by balancing it with the fountain pressure of pure 4He. The usefullness of the secondary osmotic pressure thermometer was reinvestigated.

The osmotic pressure of 3He-4He mixtures along the phase separation curve

International Nuclear Information System (INIS)

Klundert, L.J.M. van de; Bos, M.R.E.; Meij, J.A.M. van der; Steffens, H.A.

1977-01-01

The osmotic pressure of 3 He- 4 He mixtures was measured along the phase separation curve at temperatures up to 500 mK by balancing it with the fountain pressure of pure 4 He. The usefullness of the secondary osmotic pressure thermometer was reinvestigated. (Auth.)

Exchange currents in low-energy nucleon capture by 3He

International Nuclear Information System (INIS)

Wervelman, Rob.

1991-01-01

The studies described in this thesis concern absolute cross-section measurements of the radiative neutron capture reactions 3 He (n,γ) 4 He and 3 He(n,γγ) 4 He, and are complements with shell-model calculations on the radiative thermal neutron capture reaction by 3 He and on the weak 3 He(p,e + ν e ) 4 He reaction. The experiments have been performed at two neutron energies, with sub-thermal neutrons where s-wave capture is dominant, and with a quasi-monochromatic 24.5 keV neutron beam (p-wave capture). It has been found that the thermal neutron capture cross section of 3 He is 55±3 μb. Measured at 24.5 keV-neutron energy, the radiative capture cross section for p-wave neutrons turned out to be 9.1±0.8 μb. A measurement on the double-photon reaction 3 He(n th ,γγ) has yielded a cross-section value of 30±80 μb. In the theoretical part of the work a standard model of nuclear weak and electromagnetic interaction currents, consisting of a one-body impulse approximation and a two-body meson-exchange current part, has been applied to the process of nucleon capture by 3 He. Within the framework of a (0+2) (h/2π)ω shell-model calculation, using Sussex matrix elements for the 3 He and 4 He ground state wave functions, the empirical radiative cross sections for thermal neutrons of 3 He could be reproduced with satisfactory agreement. The total MEC correction to the radiative thermal neutron capture cross section is rather small because large cancellations occur between the various contributions. In the 3 He(p,e + ν e ) 4 He reaction meson-exchange contributions enlarge the astrophysical S-factor by more than a factor two. The matrix element ratio of the weak 3 He+p and the electromagnetic 3 He+n reaction is calculated to be (4.3±0.6)g A C 0 . This ratio has been found to be fairly insensitive to the percentage D-state admixtures in the 3 He and 4 He ground state wave functions. (author). 129 refs.; 24 figs.; 5 tabs

The (3He,t) and (d,2He)reactions at intermediate energies

International Nuclear Information System (INIS)

Brockstedt, A.

1987-09-01

The ( 3 He,t) reaction has been studied at 0.6-2.3 GeV at small scattering angles, 0-7 degrees, on various nuclei ( 12 C, 13 C, 26 Mg, 40 Ca, 48 Ca, 54 Fe, 90 Zr, 159 Tb, 208 Pb) including a proton target. The reaction is a single-step reaction and selects the spin-isospin channel. Angular distributions for low-lying states in 12 N are well described by DWIA calculations. From 13 C to 13 N transitions the ratio J στ /J τ , at momentum transfer, q, close to zero, is derived. The ratio remains roughly constant in the region 300 - 700 MeV/nucleon. The position of the quasi-free peak is shifted compared with free nucleon-nucleon scattering. The shift is towards higher excitation energies at q approx 1.4 fm -1 , and towards lower excitation energies at q approx 2.5 fm -1 . The p( 3 He,t)Δ ++ reaction is analysed as one-pion exchange and the ( 3 He,t) form factor is extracted. The shape and position of the Δ resonance seem to be independent of target mass for the targets studied. Compared with the p to Δ ++ transition the position is shifted towards lower excitation energy in nuclei. The (d,2p[ 1 S 0 ]) reaction, with the two protons in an 1 S 0 state labelled 2 He, is studied at 0.65 and 2.0 GeV at small angles, 0-4 degrees, on some of the targets used in the ( 3 He,t) experiment (p, 12 C, 40 Ca, 54 Fe). This reaction is also a one-step reaction that can be used for studies of spin-isospin excitations. Cross sections and tensor analysing powers are determined for the p(d, 2 He)n reaction. These results are compared with PWIA calculations. The Δ resonance in carbon is also here shifted down in excitation energy compared with the proton target. (author)

A miniature adsorption3HE refrigerator

International Nuclear Information System (INIS)

Duband, L.; Ravex, A.; Lange, A.

1991-01-01

A self-contained, recyclable laboratory 3 He refrigerator has been developed. The refrigerator is very compact, portable and is designed to be safe and reliable. The unit can easily be installed on the cold plate of a superfluid 4 He cryostat. Once bolted on the cold plate, operation of the refrigerator is controlled by a single heater. In this new design the refrigerator has a cylindrical geometry. The adsorption pump is placed above the condensation point to prevent convection during the condensation phase and to improve the pumping speed. The inhibition of convection reduces the load on the 4 He bath and increases the condensation efficiency. This refrigeration technique has great potential for space applications. The absence of moving parts makes the system reliable and vibration free. Its simplicity and the absence of external components facilitate its integration on a cryostat. In fact, a rocket-borne 3 He refrigerator has already been successfully flown and has demonstrated the feasibility of this method

Quercetin suppresses HeLa cells by blocking PI3K/Akt pathway.

Science.gov (United States)

Xiang, Tao; Fang, Yong; Wang, Shi-Xuan

2014-10-01

To explore the effect of quercetin on the proliferation and apoptosis of HeLa cells, HeLa cells were incubated with quercetin at different concentrations. Cell viability was evaluated by MTT assay, cell apoptosis was detected by Annexin-V/PI double labeled cytometry and DNA ladder assay. Cell cycle was flow cytometrically determined and the morphological changes of the cells were observed under a fluorescence microscope after Hoechst 33258 staining and the apoptosis-related proteins in the HeLa cells were assessed by Western blotting. The results showed that quercetin significantly inhibited the growth of HeLa cells and induced obvious apoptosis in vitro in a time- and dose-dependent manner. Moreover, quercetin induced apoptosis of HeLa cells in cell cycle-dependent manner because quercetin could induce arrest of HeLa cells at G0/G1 phase. Quercetin treatment down-regulated the expression of the PI3K and p-Akt. In addition, quercetin could down-regulate expression of bcl-2, up-regulate Bax, but exerted no effect on the overall expression of Akt. We are led to conclude that quercetin induces apoptosis via PI3k/Akt pathways, and quercetin has potential to be used as an anti-tumor agent against human cervix cancer.

Primary populations of metastable antiprotonic $^{4}He$ and $^{3}He$ atoms

CERN Document Server

Hori, Masaki; Hayano, R S; Ishikawa, T; Sakuguchi, J; Tasaki, T; Widmann, E; Yamaguchi, H; Torii, H A; Juhász, B; Horváth, D; Yamazaki, T

2002-01-01

Initial population distributions of metastable antiprotonic **4He and **3He atoms over principal and angular momentum quantum numbers were investigated using laser spectroscopy. The total fractions of antiprotons captured into the metastable states of the atoms were deduced. Cascade calculations were performed using the measure populations to reproduce the delayed annihilation time spectrum. Results showed agreement between the simulated and measured spectra. (Edited abstract) 30 Refs.

Effects of {Delta}-isobar degrees of freedom on the reactions {sup 3}He(n,{gamma}){sup 4}He and {sup 3}He(p,e{sup +}{nu}{sub e}){sup 4}He at low-energy

Energy Technology Data Exchange (ETDEWEB)

Schiavilla, R.

1991-12-31

The cross sections of the radiative {sup 3}He(n,{gamma}){sup 4}He and weak {sup 3}He(p,e{sup +}{nu}{sub e}){sup 4}He capture reactions at thermal neutron and keV proton energies have been calculated with the Variational Monte Carlo method. The ground state and low-energy continuum wave functions have been determined variationally from a realistic Hamiltonian, and include both nucleon and {Delta}-isobar degrees of freedom. The electroweak transition operator contains one- and two-body components in the N + {Delta} Hilbert space.

Effects of. Delta. -isobar degrees of freedom on the reactions sup 3 He(n,. gamma. ) sup 4 He and sup 3 He(p,e sup +. nu. sub e ) sup 4 He at low-energy

Energy Technology Data Exchange (ETDEWEB)

Schiavilla, R.

1991-01-01

The cross sections of the radiative {sup 3}He(n,{gamma}){sup 4}He and weak {sup 3}He(p,e{sup +}{nu}{sub e}){sup 4}He capture reactions at thermal neutron and keV proton energies have been calculated with the Variational Monte Carlo method. The ground state and low-energy continuum wave functions have been determined variationally from a realistic Hamiltonian, and include both nucleon and {Delta}-isobar degrees of freedom. The electroweak transition operator contains one- and two-body components in the N + {Delta} Hilbert space.

Geometrical aspects of reaction cross sections for 3He, 4He and 12C projectiles

International Nuclear Information System (INIS)

Ingemarsson, A.; Lantz, M.

2003-04-01

A black-disc model combined with accurate matter densities has been used for an investigation of reaction cross sections for 3 He, 4 He and 12 C projectiles. A simple relation is derived between the energy dependence of the reaction cross sections and the strength of the nucleon-nucleon interaction. A comparison is also made of the reaction cross sections for 3 He and 4 He for six different nuclei 12 C, 16 O, 40 Ca, 58,60 Ni and 208 Pb

Radiation sensitization by dihydroartemisinin on human HeLa cells of cervical cancer

International Nuclear Information System (INIS)

Chen Xialin; Cao Jianping; Ji Rong; Zhu Wei; Liu Yang; Gong Xiaomei; Tang Yan; Pan Chunyan; Fan Saijun

2009-01-01

Objective: To investigate the radiosensitizing effects of dihydroartemisinin (DHA) on human HeLa cells of cervical cancer irradiated by X rays. Methods: Cell growth kinetics was determined using MTF assay. Cell survival was analyzed by elonogenic assay. The change of cell cycle and apeptosis was measured by flow cytometry. Results: Dihydroartemisinin inhibited the growth of HeLa cells of human cervical cancer and showed a dose-dependent and time-dependent manner. Dihydroartemisinin (20 μmol/L) showed the radiosensitizing effects on HeLa cells, and the sensitizing enhancement ratio (SER) was 1.47. Dihydroartemisinin abrogated radiation-induced G 2 arrest of the tested HeLa cells, the G 2 ratio of medicine + radiation group dechned from 73.58% to 48.31%. Dihydroartemisinin enhanced the apoptosis of HeLa cells by X-irradiation, the apoptosis rates of medicine + radiation group significantly increased from 29.46%, 48.04%, 70.21% to 45.79%, 66.36% and 79.58%, respectively for 2, 4 and 6 Gy. Conclusions: Dihydroartemisinin could increase the radiosensitivity of HeLa cells of human cervical cancer. Abrogation of radiation-induced C 2 arrest could be part of the mechanism. (authors)

Vectorial transcytosis of dimeric IgA by the Calu-3 human lung epithelial cell line: upregulation by IFN-gamma

NARCIS (Netherlands)

Loman, S.; Radl, J.; Jansen, H. M.; Out, T. A.; Lutter, R.

1997-01-01

We have developed an in vitro airway epithelial cell model for dimeric immunoglobulin (Ig) A (dIgA) transcytosis that allows the assessment of polymeric Ig receptor (pIgR) gene expression and actual dIgA transport. Tight monolayers of human lung-derived Calu-3 adenocarcinoma cells grown on permeable

Immune and Inflammatory Cell Composition of Human Lung Cancer Stroma.

Directory of Open Access Journals (Sweden)

G-Andre Banat

Full Text Available Recent studies indicate that the abnormal microenvironment of tumors may play a critical role in carcinogenesis, including lung cancer. We comprehensively assessed the number of stromal cells, especially immune/inflammatory cells, in lung cancer and evaluated their infiltration in cancers of different stages, types and metastatic characteristics potential. Immunohistochemical analysis of lung cancer tissue arrays containing normal and lung cancer sections was performed. This analysis was combined with cyto-/histomorphological assessment and quantification of cells to classify/subclassify tumors accurately and to perform a high throughput analysis of stromal cell composition in different types of lung cancer. In human lung cancer sections we observed a significant elevation/infiltration of total-T lymphocytes (CD3+, cytotoxic-T cells (CD8+, T-helper cells (CD4+, B cells (CD20+, macrophages (CD68+, mast cells (CD117+, mononuclear cells (CD11c+, plasma cells, activated-T cells (MUM1+, B cells, myeloid cells (PD1+ and neutrophilic granulocytes (myeloperoxidase+ compared with healthy donor specimens. We observed all of these immune cell markers in different types of lung cancers including squamous cell carcinoma, adenocarcinoma, adenosquamous cell carcinoma, small cell carcinoma, papillary adenocarcinoma, metastatic adenocarcinoma, and bronchioloalveolar carcinoma. The numbers of all tumor-associated immune cells (except MUM1+ cells in stage III cancer specimens was significantly greater than those in stage I samples. We observed substantial stage-dependent immune cell infiltration in human lung tumors suggesting that the tumor microenvironment plays a critical role during lung carcinogenesis. Strategies for therapeutic interference with lung cancer microenvironment should consider the complexity of its immune cell composition.

186Os and 187Os enrichments and high-3He/4He sources in the Earth's mantle

DEFF Research Database (Denmark)

Brandon, A.D.; Graham, D.W.; Waight, Tod Earle

2007-01-01

at present remains core-mantle interaction. While some plumes with high 3He/4He, such as Hawaii, appear to have been subjected to detectable addition of Os (and possibly He) from the outer core, others such as Iceland do not. A positive correlation between 187Os/188Os and 3He/4He from 9.6 to 19 Ra in Iceland...

Direct harvesting of Helium-3 (3He) from heavy water nuclear reactors

International Nuclear Information System (INIS)

Bentoumi, G.; Didsbury, R.; Jonkmans, G.; Rodrigo, L.; Sur, B.

2013-01-01

The thermal neutron activation of deuterium inside a heavy-water-moderated or -cooled nuclear reactor produces a build-up of tritium in the heavy water. The in situ decay of tritium can, for certain reactor types and operating conditions, produce potentially useable amounts of 3 He, which can be directly extracted via the heavy-water cover gas without first separating, collecting and storing tritium outside the reactor. It is estimated that the amount of 3 He available for recovery from the moderator cover gas of a 700 MWe class Pressurized Heavy Water Reactor (PHWR) ranges from 0.1 to 0.7 m 3 (STP) per annum, varying with the tritium activity buildup in the moderator. The harvesting of 3 He would generate approximately 12.7 m 3 (STP) of 3 He, worth more than $30M at current market rates, over a typical 25-year operating cycle of the PHWR. This paper discusses the production of 3 He in the moderator of a PHWR and its extraction from the 4 He moderator cover gas system using conventional methods. (author)

The helium flux from the continents and ubiquity of low-3He/4He recycled crust and lithosphere

Science.gov (United States)

Day, James M. D.; Barry, Peter H.; Hilton, David R.; Burgess, Ray; Pearson, D. Graham; Taylor, Lawrence A.

2015-03-01

New helium isotope and trace-element abundance data are reported for pyroxenites and eclogites from South Africa, Siberia, and the Beni Bousera Massif, Morocco that are widely interpreted to form from recycled oceanic crustal protoliths. The first He isotope data are also presented for Archaean peridotites from the Kaapvaal (South Africa), Slave (Canada), and Siberian cratons, along with recently emplaced off-craton peridotite xenoliths from Kilbourne Hole, San Carlos (USA) and Vitim (Siberia), to complement existing 3He/4He values obtained for continental and oceanic peridotites. Helium isotope compositions of peridotite xenoliths vary from 7.3 to 9.6 RA in recently (volcanics that contain a contribution from asthenospheric sources. Using the new He isotope data for cratonic peridotites and assuming that significant portions (>50%) of the Archaean and Proterozoic continental lithospheric mantle are stable and unaffected by melt or fluid infiltration on geological timescales (>0.1 Ga), and that U and Th contents vary between cratonic lithosphere and non-cratonic lithosphere, calculations yield a 3He flux of 0.25-2.2 atoms/s/cm2 for the continental lithospheric mantle. These estimates differ by a factor of ten from non-cratonic lithospheric mantle and are closer to the observed 3He flux from the continents (<1 atoms/s/cm2). Pyroxenites and eclogites from the continental regions are all characterized by 3He/4He (0.03-5.6 RA) less than the depleted upper mantle, and relatively high U and Th contents. Together with oceanic and continental lithospheric peridotites, these materials represent reservoirs with low time-integrated 3He/(U + Th) in the mantle. Pyroxenites and eclogites are also characterized by higher Fe/Mg, more radiogenic Os-Pb isotope compositions, and more variable δ18O values (∼3‰ to 7‰), compared with peridotitic mantle. These xenoliths are widely interpreted to be the metamorphic/metasomatic equivalents of recycled oceanic crustal protoliths. The

Low-temperature H2-4He and H2-3He targets for operation on an electron beam

International Nuclear Information System (INIS)

Gol'dshtejn, V.A.; Lubyanyj, V.V.

1981-01-01

Structures and basic characteristics of H 2 - 4 He and H 2 - 3 He low temperature targets are given. Technique of 3 He target filling is described. Initial target cooling up to liquid 4 He temperature and its filling up take near approximately 1 h, at that 4 He flow rate equals 15 l. Repeated filling up of 4 He takes 20 min, and target filling up with 3 He - 10-15 min. Good thermal insulation of a cryostat and targets permits the 4 He target to be operated with an electron beam of a mean current of up to 0.5 μA without filling up 4 He for 70 h. At that flow rate of liquid 4 He amounts to 0.2 l/h, and liquid hydrogen - 0.04 l/h. It is concluded that H 2 - 4 He and H 2 - 3 He targets are reliable and simple in operation and permit to work with accelerated particle beams of intensity corresponding to power release >= 0.5 W without corrections for density change [ru

Probing HeII Reionization at z>3.5 with Resolved HeII Lyman Alpha Forest Spectra

Science.gov (United States)

Worseck, Gabor

2017-08-01

The advent of GALEX and COS have revolutionized our view of HeII reionization, the final major phase transition of the intergalactic medium. COS spectra of the HeII Lyman alpha forest have confirmed with high confidence the high HeII transmission that signifies the completion of HeII reionization at z 2.7. However, the handful of z>3.5 quasars observed to date show a set of HeII transmission 'spikes' and larger regions with non-zero transmission that suggest HeII reionization was well underway by z=4. This is in striking conflict with predictions from state-of-the-art radiative transfer simulations of a HeII reionization driven by bright quasars. Explaining these measurements may require either faint quasars or more exotic sources of hard photons at z>4, with concomitant implications for HI reionization. However, many of the observed spikes are unresolved in G140L spectra and are significantly impacted by Poisson noise. Current data cannot reliably probe the ionization state of helium at z>3.5.We request 41 orbits to obtain science-grade G130M spectra of the two UV-brightest HeII-transmitting QSOs at z>3.5 to confirm and resolve their HeII transmission spikes as an unequivocal test of early HeII reionization. These spectra are complemented by recently obtained data from 8m telescopes: (1) Echelle spectra of the coeval HI Lya forest to map the underlying density field that modulates the HeII absorption, and (2) Our dedicated survey for foreground QSOs that may source the HeII transmission. Our recent HST programs revealed the only two viable targets to resolve the z>3.5 HeII Lyman alpha forest, and to conclusively solve this riddle.

Tiny plastic lung mimics human pulmonary function

Science.gov (United States)

Careers Inclusion & Diversity Work-Life Balance Career Resources Apply for a Job Postdocs Students Goals Recycling Green Purchasing Pollution Prevention Reusing Water Resources Environmental Management Releases - 2016 » April » Tiny plastic lung mimics human pulmonary function Tiny plastic lung mimics

The effect of Demkov coupling in the rotational predissociation of 3He4He+, ch. 4

International Nuclear Information System (INIS)

Asselt, N.P.F.B. van; Maas, J.G.; Los, J.

1976-01-01

The momentum distribution of the 3 He + and the 4 He + fragments, both originating from rotational predissociation of 3 He 4 He + has been measured. There is a pronounced difference between the intensity distributions in both spectra. This difference is explained as the result of a Demkov coupling between the two lowest electronic states of the molecular ion. The rotational quantum numbers of the states involved are calculated from the spectra

Anti-lung cancer effects of novel ginsenoside 25-OCH(3)-PPD.

Science.gov (United States)

Wang, Wei; Rayburn, Elizabeth R; Hang, Jie; Zhao, Yuqing; Wang, Hui; Zhang, Ruiwen

2009-09-01

20(S)-25-methoxyl-dammarane-3beta, 12beta, 20-triol (25-OCH(3)-PPD), a newly identified natural product from Panax notoginseng, exhibits activity against a variety of cancer cells. Herein, we report the effects of this compound on human A549, H358, and H838 lung cancer cells, and compare these effects with a control lung epithelial cell line, BEAS-2B. 25-OCH(3)-PPD decreased survival, inhibited proliferation, and induced apoptosis and G1 cell cycle arrest in the lung cancer cell lines. The P. notoginseng compound also decreased the levels of proteins associated with cell proliferation and cell survival. Moreover, 25-OCH(3)-PPD inhibited the growth of A549 lung cancer xenograft tumors. 25-OCH(3)-PPD demonstrated low toxicity to non-cancer cells, and no observable toxicity was seen when the compound was administered to animals. In conclusion, our preclinical data indicate that 25-OCH(3)-PPD is a potential therapeutic agent in vitro and in vivo, and further preclinical and clinical development of this agent for lung cancer is warranted.

Human models of acute lung injury

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Alastair G. Proudfoot

2011-03-01

Full Text Available Acute lung injury (ALI is a syndrome that is characterised by acute inflammation and tissue injury that affects normal gas exchange in the lungs. Hallmarks of ALI include dysfunction of the alveolar-capillary membrane resulting in increased vascular permeability, an influx of inflammatory cells into the lung and a local pro-coagulant state. Patients with ALI present with severe hypoxaemia and radiological evidence of bilateral pulmonary oedema. The syndrome has a mortality rate of approximately 35% and usually requires invasive mechanical ventilation. ALI can follow direct pulmonary insults, such as pneumonia, or occur indirectly as a result of blood-borne insults, commonly severe bacterial sepsis. Although animal models of ALI have been developed, none of them fully recapitulate the human disease. The differences between the human syndrome and the phenotype observed in animal models might, in part, explain why interventions that are successful in models have failed to translate into novel therapies. Improved animal models and the development of human in vivo and ex vivo models are therefore required. In this article, we consider the clinical features of ALI, discuss the limitations of current animal models and highlight how emerging human models of ALI might help to answer outstanding questions about this syndrome.

Effect of increases in lung volume on clearance of aerosolized solute from human lungs

Energy Technology Data Exchange (ETDEWEB)

Marks, J.D.; Luce, J.M.; Lazar, N.M.; Wu, J.N.; Lipavsky, A.; Murray, J.F.

1985-10-01

To study the effect of increases in lung volume on solute uptake, we measured clearance of /sup 99m/Tc-diethylenetriaminepentaacetic acid (Tc-DTPA) at different lung volumes in 19 healthy humans. Seven subjects inhaled aerosols (1 micron activity median aerodynamic diam) at ambient pressure; clearance and functional residual capacity (FRC) were measured at ambient pressure (control) and at increased lung volume produced by positive pressure (12 cmH2O continuous positive airway pressure (CPAP)) or negative pressure (voluntary breathing). Six different subjects inhaled aerosol at ambient pressure; clearance and FRC were measured at ambient pressure and CPAP of 6, 12, and 18 cmH2O pressure. Six additional subjects inhaled aerosol at ambient pressure or at CPAP of 12 cmH2O; clearance and FRC were determined at CPAP of 12 cmH2O. According to the results, Tc-DTPA clearance from human lungs is accelerated exponentially by increases in lung volume, this effect occurs whether lung volume is increased by positive or negative pressure breathing, and the effect is the same whether lung volume is increased during or after aerosol administration. The effect of lung volume must be recognized when interpreting the results of this method.

Effect of increases in lung volume on clearance of aerosolized solute from human lungs

International Nuclear Information System (INIS)

Marks, J.D.; Luce, J.M.; Lazar, N.M.; Wu, J.N.; Lipavsky, A.; Murray, J.F.

1985-01-01

To study the effect of increases in lung volume on solute uptake, we measured clearance of /sup 99m/Tc-diethylenetriaminepentaacetic acid (Tc-DTPA) at different lung volumes in 19 healthy humans. Seven subjects inhaled aerosols (1 micron activity median aerodynamic diam) at ambient pressure; clearance and functional residual capacity (FRC) were measured at ambient pressure (control) and at increased lung volume produced by positive pressure [12 cmH 2 O continuous positive airway pressure (CPAP)] or negative pressure (voluntary breathing). Six different subjects inhaled aerosol at ambient pressure; clearance and FRC were measured at ambient pressure and CPAP of 6, 12, and 18 cmH 2 O pressure. Six additional subjects inhaled aerosol at ambient pressure or at CPAP of 12 cmH 2 O; clearance and FRC were determined at CPAP of 12 cmH 2 O. According to the results, Tc-DTPA clearance from human lungs is accelerated exponentially by increases in lung volume, this effect occurs whether lung volume is increased by positive or negative pressure breathing, and the effect is the same whether lung volume is increased during or after aerosol administration. The effect of lung volume must be recognized when interpreting the results of this method

Energy dependence and temporal evolution of the 3He/4He ratios in heavy-ion-rich energetic particle events

International Nuclear Information System (INIS)

Moebius, E.; Hovestadt, D.; Klecker, B.; Gloeckler, G.

1980-01-01

The energy dependence of the 3 He/ 4 He ratio between 0.44 and 4.1 MeV per nucleon has been studied for six heavy-ion--rich events observed in 1974 and 1976 using the low-energy dE/dx versus E Ultralow-Energy Particle telescope (ULET) on IMP 8. We find that all selected heavy-ion--rich events are also enriched in 3 He, that the 3 He/ 4 He He ratio decreases with decreasing energies, and that a rapid temporal evolution of the 3 He/ 4 He and the Fe/(H+He) ratios is strongly correlated during one event with the maximum value at the onset. These results are discussed in terms of a model which is based on preferential injection of 3 He and Fe resulting from turbulent ion heating and subsequent Fermi acceleration

The production of He-3 and heavy ion enrichment in He-3-rich flares by electromagnetic hydrogen cyclotron waves

Science.gov (United States)

Temerin, M.; Roth, I.

1992-01-01

A new model is presented for the production of He-3 and heavy ion enrichments in He-3-rich flares using a direct single-stage mechanism. In analogy with the production of electromagnetic hydrogen cyclotron waves in earth's aurora by electron beams, it is suggested that such waves should exist in the electron acceleration region of impulsive solar flares. Both analytic and test-particle models of the effect of such waves in a nonuniform magnetic field show that these waves can selectively accelerate He-3 and heavy ions to MeV energies in a single-stage process, in contrast to other models which require a two-stage mechanism.

Bulk damping of sound in superfluid 3He--4He under stagnation of the normal component

International Nuclear Information System (INIS)

Karchava, T.A.; Sanikidze, D.G.; Chkhaidze, N.D.

1983-01-01

The propagation of waves in superfluid 3 He-- 4 He solutions is considered under partial stagnation of the normal component. The wave processes in capillaries are presented as a superposition of the first sound, second sound, and viscous and diffusion waves. The damping coefficients are calculated for the modified first sound and for the thermal wave in superfluid 3 He-- 4 He solutions and related to the viscosity, thermal conductivity, diffusion, barodiffusion, and thermodiffusion coefficients

Lagrangian of superfluid 3He

International Nuclear Information System (INIS)

Theodorakis, S.

1988-01-01

This paper presents a phenomenological Lagrangian that fully describes the dynamics of any homogeneous phase of superfluid 3 He, unitary or not, omitting relaxation. This Lagrangian is built by using the concept of a local SO(3) x SO(3) x U(1) symmetry. The spin and angular momentum play the role of gauge fields. We derive the Leggett equations for spin and orbital dynamics from the equations of motion, for both the A and the B phase. This Lagrangian not only enables us to describe both the spin and orbital dynamics of superfluid 3 He in a unified fashion, but can also be used for finding the dynamics in any experimental situation. Furthermore, it can describe the dynamics of the magnitude, as well as of the orientation of the order parameter, and thus it can be used to describe the dynamics of the A-B phase transition

The Cosmic Abundance of 3He: Green Bank Telescope Observations

Science.gov (United States)

Balser, Dana; Bania, Thomas

2018-01-01

The Big Bang theory for the origin of the Universe predicts that during the first ~1,000 seconds significant amounts of the light elements (2H, 3He, 4He, and 7Li) were produced. Many generations of stellar evolution in the Galaxy modifies these primordial abundances. Observations of the 3He+ hyperfine transition in Galactic HII regions reveals a 3He/H abundance ratio that is constant with Galactocentric radius to within the uncertainties, and is consistent with the primordial value as determined from cosmic microwave background experiments (e.g., WMAP). This "3He Plateau" indicates that the net production and destruction of 3He in stars is approximately zero. Recent stellar evolution models that include thermohaline mixing, however, predict that 3He/H abundance ratios should slightly decrease with Galactocentric radius, or in places in the Galaxy with lower star formation rates. Here we discuss sensitive Green Bank Telescope (GBT) observations of 3He+ at 3.46 cm in a subset of our HII region sample. We develop HII region models and derive accurate 3He/H abundance ratios to better constrain these new stellar evolution models.

Progression of emphysema evaluated by MRI using hyperpolarized (3)He (HP (3)He) measurements in patients with alpha-1-antitrypsin (A1AT) deficiency compared with CT and lung function tests

DEFF Research Database (Denmark)

Stavngaard, T; Søgaard, L Vejby; Batz, M

2009-01-01

BACKGROUND: The progression of emphysema is traditionally measured by pulmonary function test, with forced expiratory volume in 1 s (FEV(1)) being the most accepted and used measurement. However, FEV(1) is insensitive in detecting mild/slow progression of emphysema because of low reproducibility...... as compared to yearly decline. PURPOSE: To investigate the progression of emphysema over a period of 2 years using diffusion-weighted hyperpolarized (HP) (3)He magnetic resonance imaging (MRI) in patients with alpha-1-antitrypsin (A1AT) deficiency. MATERIAL AND METHODS: Nine patients with severe A1AT...... density [CT-PD15] and relative area of emphysema below -910 HU [CT-RA-910]). RESULTS: Seven patients were scanned three times, one patient two times, and one patient only at baseline. The mean increase in ADC values from first to last HP (3)He MR scanning was 3.8% (0.014 cm(2)/s [SD 0.024 cm(2)/s...

Modulation of intracellular calcium homeostasis by trimethyltin chloride in human tumour cells: Neuroblastoma SY5Y and cervix adenocarcinoma HeLa S3

International Nuclear Information System (INIS)

Florea, Ana-Maria; Splettstoesser, Frank; Dopp, Elke; Rettenmeier, Albert W.; Buesselberg, Dietrich

2005-01-01

Physiological modifications of intracellular Ca 2+ ([Ca 2+ ] i ) levels trigger and/or regulate a diversity of cellular activities (e.g. neurotransmitter release, synaptic plasticity, muscular contraction, cell proliferation), while calcium overloads could result in cytotoxicity. Previously, we have shown that trimethyltin chloride (Me 3 SnCl; TMT) modulates calcium homeostasis in cervix adenocarcinoma (HeLa S3) cells [Florea, A.-M., Dopp, E., Buesselberg, D., 2005. TMT induces elevated calcium transients in HeLa cells: types and levels of response. Cell Calcium 37, 252-258]. Here we compare [Ca 2+ ] i -changes induced by trimethyltin chloride in neuroblastoma SY5Y and HeLa S3 cells using calcium-sensitive dyes (fluo-4/AM (fluo-4) and rhod-2/AM (rhod-2)) and laser scanning microscopy (LSM). TMT-induced calcium elevations in neuroblastoma SY5Y as well as in HeLa S3 cells. [Ca 2+ ] i rose to a sustained plateau or to transient spikes. Overall, the detected averaged increase of the maximum calcium elevation were: 0.5 μM ∼125.6%; 5 μM ∼130.1%; 500 μM ∼145% in HeLa S3 cells and 0.5 μM ∼133.3%; 5 μM ∼136.1%; 500 μM ∼147.1% in neuroblastoma SY5Y cells. The calcium rise derived from internal stores did not significantly depend on the presence of calcium in the external solution: ∼109% (no calcium added) versus ∼117% (2 mM calcium; 5 μM TMT) in HeLa cells. This difference was similar in neuroblastoma SY5Y cells, were ∼127% versus ∼136% increase (5 μM TMT) were measured. Staining of calcium stores with rhod-2 showed a TMT-induced [Ca 2+ ] i -decrease in the stores followed by an increase of the calcium concentration in the nuclei of the two cell lines tested. Our results suggest that toxic effects in human tumour cells after exposure to trimethyltin compounds might be due to an elevation of [Ca 2+ ] i

Calculations of static properties of spin-polarized 3He--4He mixtures

International Nuclear Information System (INIS)

Owers-Bradley, J.R.; Bowley, R.M.; Main, P.C.

1985-01-01

The theory of dilute mixtures of 3 He in 4 He that have been polarized by a strong magnetic field is developed. The interaction between the quasiparticles is taken to be constant, an approximation valid at low temperatures. The polarization of the mixture depends on the strength of the interaction. The internal energy, the specific heat, the osmotic pressure, and the velocity of second sound are also calculated. The specific heat is relatively insensitive to the interaction, but it does change significantly with magnetic field. The osmotic pressure is more sensitive to the effects of the interaction for some 3 He concentrations and temperatures. The velocity of second sound behaves qualitatively like the osmotic pressure. The measurement of these quantities as a function of temperature and magnetic field is discussed with a view to obtaining the strength of the interaction between quasiparticles

Experimental studies on lung carcinogenesis and their relationship to future research on radiation-induced lung cancer in humans

International Nuclear Information System (INIS)

Cross, F.T.

1991-03-01

The usefulness of experimental systems for studying human lung carcinogenesis lies in the ease of studying components of a total problem. As an example, the main thrust of attack on possible synergistic interactions between radiation, cigarette smoke, and other irritants must be by means of research on animals. Because animals can be serially sacrificed, a systematic search can be made for progressive lung changes, thereby improving our understanding of carcinogenesis. The mechanisms of radiation-induced carcinogenesis have not yet been delineated, but modern concepts of molecular and cellular biology and of radiation dosimetry are being increasingly applied to both in vivo and in vitro exposure to determine the mechanisms of radiation-induced carcinogenesis, to elucidate human data, and to aid in extrapolating experimental animal data to human exposures. In addition, biologically based mathematical models of carcinogenesis are being developed to describe the nature of the events leading to malignancy; they are also an essential part of a rational approach to quantitative cancer risk assessment. This paper summarizes recent experimental and modeling data on radon-induced lung cancer and includes the confounding effects of cigarette-smoke exposures. The applicability of these data to understanding human exposures is emphasized, and areas of future research on human radiation-induced carcinogenesis are discussed. 7 refs., 2 figs., 3 tabs

On the 3He anomaly in hot subdwarf B stars

Science.gov (United States)

Schneider, David; Irrgang, Andreas; Heber, Ulrich; Nieva, Maria F.; Przybilla, Norbert

2017-12-01

Decades ago, 3He isotope enrichment in helium-weak B-type main-sequence, in blue horizontal branch and in hot subdwarf B (sdB) stars, i.e., helium-core burning stars of the extreme horizontal branch, were discovered. Diffusion processes in the atmosphere of these stars lead to the observed abundance anomalies. Quantitative spectral analyses of high-resolution spectra to derive photospheric isotopic helium abundance ratios for known 3He sdBs have not been performed yet. We present preliminary results of high-resolution and high S/N spectra to determine the 3He and 4He abundances of nine known 3He sdBs. We used a hybrid local/non-local thermodynamic equilibrium (LTE/NLTE) approach for B-type stars investigating multiple He i lines, including λ4922 Å and λ6678 Å, which show the strongest isotopic shifts in the optical spectral range.We also report the discovery of four new 3He sdBs from the ESO Supernova Progenitor survey. Most of the 3He sdBs cluster in a narrow temperature strip between ˜ 26000 K and ˜ 30000 K and have almost no atmospheric 4He at all. Interestingly, three 3He sdBs show evidence for vertical helium stratification.

On the 3He anomaly in hot subdwarf B stars

Directory of Open Access Journals (Sweden)

Schneider David

2017-12-01

Full Text Available Decades ago, 3He isotope enrichment in helium-weak B-type main-sequence, in blue horizontal branch and in hot subdwarf B (sdB stars, i.e., helium-core burning stars of the extreme horizontal branch, were discovered. Diffusion processes in the atmosphere of these stars lead to the observed abundance anomalies. Quantitative spectral analyses of high-resolution spectra to derive photospheric isotopic helium abundance ratios for known 3He sdBs have not been performed yet. We present preliminary results of high-resolution and high S/N spectra to determine the 3He and 4He abundances of nine known 3He sdBs. We used a hybrid local/non-local thermodynamic equilibrium (LTE/NLTE approach for B-type stars investigating multiple He i lines, including λ4922 Å and λ6678 Å, which show the strongest isotopic shifts in the optical spectral range.We also report the discovery of four new 3He sdBs from the ESO Supernova Progenitor survey. Most of the 3He sdBs cluster in a narrow temperature strip between ∼ 26000 K and ∼ 30000 K and have almost no atmospheric 4He at all. Interestingly, three 3He sdBs show evidence for vertical helium stratification.

HE4 Tissue Expression and Serum HE4 Levels in Healthy Individuals and Patients with Benign or Malignant Tumors

DEFF Research Database (Denmark)

Karlsen, Nikoline S; Karlsen, Mona A; Høgdall, Claus K

2014-01-01

, this review aims to systematically outline published results of HE4 tissue expression and serum HE4 levels in healthy individuals and patients with benign or malignant tumors. Our findings suggest scientific basis for a potential diagnostic ability of HE4 in gynecologic cancer and lung cancer, and further...

Regulator of G-protein signaling 5 (RGS5) inhibits cell proliferation and enhances radiosensitivity of human lung cancer cells

International Nuclear Information System (INIS)

Xu Zumin; Wang Jin; Zuo Yufang; Yu Zhonghua; Peng Fang; Hu Xiao; Zhou Qichao; Ma Honglian; Bao Yong; Chen Ming

2014-01-01

Objective: To investigate the effects of regulator and the underlying molecular mechanisms of G-protein signaling 5 (RGS5) on radiation response in human lung cancer cells. Methods: The effects of RGS5 on viability were determined by MTT assay, and apoptosis rate was detected by flow cytometry, in human lung cancer cells. The combined effect of ionizing radiation and RGS5 on tumor cells was detected by colony formation assay. The protein expression was detected by Western blot. Results: RGS5 overexpression remarkably inhibited the survival of human lung cancer cells, and the growth inhibition rate of RGS5 overexpression on A549 and Calu-3 cells were 44.4% (F = 29.18, P < 0.05) and 39.27% (F = 23.04, P < 0.05) at 48 h, and 54.3%(F = 103.45, P < 0.05), 44.7%(F = 108.02, P < 0.05) at 72 h post-irradiation, respectively. RGS5 might exert its inhibitory effects on human lung cancer cells by inducing tumor cell apoptosis, while the apoptotic cells rate in A549 and Calu-3 cells in control group, pTRiEX group and pTRiEX-RGS5 group were (1.3±0.2)%, (3.4±0.6)%, (19.6±2.3)% (F = 86.62, P < 0.05), and (3.2±0.8)%, (3.0±0.9)%, (12.8±1.8)% (F = 28.80, P < 0.05) at 36 h post-irradiation, respectively. Furthermore, RGS5 could sensitize the lung cancer cells to radiation. Conclusions: RGS5 might play an inhibitory role in human lung cancer cell proliferation, which may explain the pathoclinical observation thet high expression of RGSS is a favorable prognostic factor in NSCLC patients. In addition, RGS5 also enhance the anti-tumor effects of radiation in human lung cancer cells. (authors)

Emphysematous changes and normal variation in smokers and COPD patients using diffusion 3He MRI

International Nuclear Information System (INIS)

Swift, Andrew J.; Wild, Jim M.; Fichele, Stan; Woodhouse, Neil; Fleming, Sally; Waterhouse, Judith; Lawson, Rod A.; Paley, Martyn N.J.; Van Beek, Edwin J.R.

2005-01-01

Introduction: This study aims to quantify global and regional changes of diffusive motion of 3 He gas within the lung, as determined by hyperpolarized 3 He MR apparent diffusion coefficient (ADC) measurement, in non-smokers, smokers and chronic obstructive pulmonary disease (COPD) patients. Methods: Age-matched groups of six healthy non-smokers, five healthy smokers and five patients with COPD. The experiments were performed with approval from the local Research Ethics Committee. Diffusion imaging was performed following hyperpolarized 3 He gas inhalation, producing ADC maps. Mean and standard deviation of the ADCs were used to compare the subject groups and assess regional variations within individuals. Results: The intra-individual standard deviation of ADC in the healthy smokers was significantly larger than that of the non-smoking group (P < 0.02). Compared to the non-smoking group, COPD patients had significantly higher mean and standard deviation of ADC (P < 0.01). The mean ADC in the anterior half of the chest was systematically higher than in the posterior half in the healthy non-smoking subject group. Discussion: This study suggests that there are regional trends in the ADC values of healthy volunteers that may have implications for the clinical interpretation of ADC values. Less homogeneous ADC values have been detected in asymptomatic smokers, indicative of damage to the distal air spaces

/sup 3/He functions in tokamak-pumped laser systems

Energy Technology Data Exchange (ETDEWEB)

Jassby, D.L.

1986-10-01

/sup 3/He placed in an annular cell around a tokamak fusion generator can convert moderated fusion neutrons to energetic ions by the /sup 3/He(n,p)T reaction, and thereby excite gaseous lasants mixed with the /sup 3/He while simultaneously breeding tritium. The total /sup 3/He inventory is about 4 kg for large tokamak devices. Special configurations of toroidal-field magnets, neutron moderators and beryllium reflectors are required to permit nearly uniform neutron current into the laser cell with minimal attenuation. The annular laser radiation can be combined into a single output beam at the top of the tokamak.

The new phases of liquid 3He

International Nuclear Information System (INIS)

Leggett, A.J.

1976-01-01

Comments are made on two new phases of liquid 3 He, referred to as 3 He-A and 3 He-B, discovered in the temperature region below 3 mk. It has been assumed that these new phases are anisotropic superfluids, and a general picture of them is presented, which has been successful in explaining at least qualitatively, many of the static, dynamic and transport properties of the new phases. Whilst the qualitative behaviour is in good agreement with theory there are, however, some quantitative discrepancies. In many cases these may be due to orientational and geometric effects not yet understood. One of the most fruitful areas for comparison of theory and experiment is the NMR behaviour and the dynamic nuclear magnetism. The anomalous behaviour observed arises because the nuclear dipole energy, although very small, can act coherently in the superfluid state. (U.K.)

4-Methoxyestradiol-induced oxidative injuries in human lung epithelial cells

International Nuclear Information System (INIS)

Cheng Yahsin; Chang, Louis W.; Cheng Lichuan; Tsai, M.-H.; Lin Pinpin

2007-01-01

Epidemiological studies indicated that people exposed to dioxins were prone to the development of lung diseases including lung cancer. Animal studies demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) increased liver tumors and promoted lung metaplasia in females. Metabolic changes in 17β-estradiol (E 2 ) resulted from an interaction between TCDD and E 2 could be associated with gender difference. Previously, we reported that methoxylestradiols (MeOE 2 ), especially 4-MeOE 2 , accumulated in human lung cells (BEAS-2B) co-treated with TCDD and E 2 . In the present study, we demonstrate unique accumulation of 4-MeOE 2 , as a result of TCDD/E 2 interaction and revealed its bioactivity in human lung epithelial cell line (H1355). 4-Methoxyestradiol treatment significantly decreased cell growth and increased mitotic index. Elevation of ROS and SOD activity, with a concomitant decrease in the intracellular GSH/GSSG ratio, was also detected in 4-MeOE 2 -treated cells. Quantitative comet assay showed increased oxidative DNA damage in the 4-MeOE 2 -treated H1355 cells, which could be significantly reduced by the anti-oxidant N-acetylcysteine (NAC). However, inhibition of cell growth and increase in mitotic arrest induced by 4-MeOE 2 were unaffected by NAC. We concluded that 4-MeOE 2 accumulation resulting from TCDD and E 2 interaction would contribute to the higher vulnerability on lung pathogenesis in females when exposed to TCDD

Apoptotic induction activity of Dactyloctenium aegyptium (L. P.B. and Eleusine indica (L. Gaerth. extracts on human lung and cervical cancer cell lines

Directory of Open Access Journals (Sweden)

Pintusorn Hansakul

2009-08-01

Full Text Available Dactyloctenium aegyptium (L. P.B. (Yaa paak khwaai and Eleusine indica (L. Gaerth. (Yaa teen-ka have long been used in traditional Thai medicine because of their diuretic, anti-inflamatory, and antipyretic effects. The present study examined the antiproliferative and cytotoxic effects of the hexane and butanolic extracts of these two grass species. All the grass extracts exhibited selective growth inhibition effect on human lung cancer (A549 and cervical cancer (HeLa cells relative to normal human lung MRC-5 fibroblasts with IC50 values in a range of 202 to 845 mg/ml. Apparently, HeLa cellswere more sensitive to the extracts than A549 cells. Moreover, all the extracts induced lethality in both cancer cell lines atconcentrations close to 1,000 mg/ml, indicating their selective cytotoxicity effects. ELISA assay showed that only the hexaneextract of D. aegyptium (L. P.B. and E. indica (L. Gaerth. significantly increased the apoptotic level in extract-treatedA549 cells. However, DNA ladder assay detected classic DNA ladder patterns, a characteristic feature of apoptosis, in both cancer cell lines treated with all the extracts in a dose- and time-dependent manner. Taken together, these results indicatethat the cytotoxic activity of the grass extracts against lung and cervical cancer cells is mediated through the induction ofapoptosis.

Effects of epidermal growth factor, transferrin, and insulin on lipofection efficiency in human lung carcinoma cells.

Science.gov (United States)

Yanagihara, K; Cheng, H; Cheng, P W

2000-01-01

Poor transfection efficiency is the major drawback of lipofection. We showed previously that addition of transferrin (TF) to Lipofectin enhanced the expression of a reporter gene in HeLa cells by 120-fold and achieved close to 100% transfection efficiency. The purpose of this study was to determine whether TF and other ligands could improve the efficiency of lipofection in lung carcinoma cells. Confluent A549, Calu3, and H292 cells were transfected for 18 hours with a plasmid DNA (pCMVlacZ) using Lipofectin plus TF, insulin, or epidermal growth factor as the vector. The transfected cells were assessed for transfection efficiency by beta-galactosidase activity (light units/microg protein) and the percentage of blue cells following 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside staining. Lipofectin supplemented with epidermal growth factor yielded the largest enhancement of lipofection efficiency (lipofection efficiency in A549 and Calu3 cells but not in H292 cells, whereas TF showed significant lipofection efficiency-enhancing effect in Calu3 and H292 cells but not in A549 cells. The transfection efficiency correlated well with the amounts of DNA delivered to the nucleus as well as the amounts of the receptor. These results indicate that the gene delivery strategy employing ligand-facilitated lipofection can achieve high transfection efficiency in human lung carcinoma cells. In addition, enhancement of the expression of the receptor may be a possible strategy for increasing the efficiency of gene targeting.

A validation of the 3H/3He method for determining groundwater recharge

Science.gov (United States)

Solomon, D. K.; Schiff, S. L.; Poreda, R. J.; Clarke, W. B.

1993-09-01

Tritium and He isotopes have been measured at a site where groundwater flow is nearly vertical for a travel time of 100 years and where recharge rates are spatially variable. Because the mid-1960s 3H peak (arising from aboveground testing of thermonuclear devices) is well-defined, the vertical groundwater velocity is known with unusual accuracy at this site. Utilizing 3H and its stable daughter 3He to determine groundwater ages, we compute a recharge rate of 0.16 m/yr, which agrees to within about 5% of the value based on the depth of the 3H peak (measured both in 1986 and 1991) and two-dimensional modeling in an area of high recharge. Zero 3H/3He age occurs at a depth that is approximately equal to the average depth of the annual low water table, even though the capillary fringe extends to land surface during most of the year at the study site. In an area of low recharge (0.05 m/yr) where the 3H peak (and hence the vertical velocity) is also well-defined, the 3H/3He results could not be used to compute recharge because samples were not collected sufficiently far above the 3H peak; however, modeling indicates that the 3H/3He age gradient near the water table is an accurate measure of vertical velocities in the low-recharge area. Because 3H and 3He have different diffusion coefficients, and because the amount of mechanical mixing is different in the area of high recharge than in the low-recharge area, we have separated the dispersive effects of mechanical mixing from molecular diffusion. We estimate a longitudinal dispersivity of 0.07 m and effective diffusion coefficients for 3H (3HHO) and 3He of 2.4×10-5 and 1.3×10-4 m2/day, respectively. Although the 3H/3He age gradient is an excellent indicator of vertical groundwater velocities above the mid-1960s 3H peak, dispersive mixing and diffusive loss of 3He perturb the age gradient near and below the 3H peak.

Impact of field number and beam angle on functional image-guided lung cancer radiotherapy planning

Science.gov (United States)

Tahir, Bilal A.; Bragg, Chris M.; Wild, Jim M.; Swinscoe, James A.; Lawless, Sarah E.; Hart, Kerry A.; Hatton, Matthew Q.; Ireland, Rob H.

2017-09-01

To investigate the effect of beam angles and field number on functionally-guided intensity modulated radiotherapy (IMRT) normal lung avoidance treatment plans that incorporate hyperpolarised helium-3 magnetic resonance imaging (3He MRI) ventilation data. Eight non-small cell lung cancer patients had pre-treatment 3He MRI that was registered to inspiration breath-hold radiotherapy planning computed tomography. IMRT plans that minimised the volume of total lung receiving  ⩾20 Gy (V20) were compared with plans that minimised 3He MRI defined functional lung receiving  ⩾20 Gy (fV20). Coplanar IMRT plans using 5-field manually optimised beam angles and 9-field equidistant plans were also evaluated. For each pair of plans, the Wilcoxon signed ranks test was used to compare fV20 and the percentage of planning target volume (PTV) receiving 90% of the prescription dose (PTV90). Incorporation of 3He MRI led to median reductions in fV20 of 1.3% (range: 0.2-9.3% p  =  0.04) and 0.2% (range: 0 to 4.1%; p  =  0.012) for 5- and 9-field arrangements, respectively. There was no clinically significant difference in target coverage. Functionally-guided IMRT plans incorporating hyperpolarised 3He MRI information can reduce the dose received by ventilated lung without comprising PTV coverage. The effect was greater for optimised beam angles rather than uniformly spaced fields.

Hyperpolarized 3He apparent diffusion coefficient MRI of the lung: reproducibility and volume dependency in healthy volunteers and patients with emphysema

DEFF Research Database (Denmark)

Diaz, S.; Casselbrant, I.; Piitulainen, E.

2008-01-01

PURPOSE: To measure the apparent diffusion coefficient (ADC) of hyperpolarized (HP) (3)He gas using diffusion weighted MRI in healthy volunteers and patients with emphysema and examine the reproducibility and volume dependency. MATERIALS AND METHODS: A total of eight healthy volunteers and 16...... patients with emphysema were examined after inhalation of HP (3)He gas mixed with nitrogen (N(2)) during breathhold starting from functional residual capacity (FRC) in supine position. Coronal diffusion-sensitized MR images were acquired. Each subject was imaged on three separate days over a seven...... in mean ADC with increased inhaled gas volume was observed in both groups. CONCLUSION: Mean ADC and SD of HP (3)He MRI is reproducible and discriminates well between healthy controls and patients with emphysema at the higher gas volume. This method is robust and may be useful to gain new insights...

In-vivo counting of 241Am in human lungs and tracheobronchial lymph nodes

International Nuclear Information System (INIS)

Northcutt, A.R.; Binney, S.E.; Palmer, H.E.

1988-01-01

A study was conducted of a human male who had inhaled a mixture of 241 Am and Pu. To distinguish 241 Am deposited in the subject's lungs from translocated activity deposited in the tracheobronchial lymph nodes (TBLN), two intrinsic Ge detectors were collimated with 0.3-cm Pb sheeting. A tissue-equivalent phantom containing either 22.9 kBq (620 nCi) of 241 Am in the lungs or a 81.4 kBq (2200 nCi) 241 Am point source in the TBLN was measured. Calibration curves observed from lateral differential scans on the phantom were compared to data obtained by the same detection system for a human male with a measured lung deposition of 89 Bq (2.4 nCi) of 241 Am. Comparison of the human data to the calibration curves indicated the activity was restricted primarily to the lungs. The calibration curves demonstrate that this method is useful in determining the distribution of inhaled radioactivity between the lungs and TBLN. The measured activity from the male subject generally supported the ICRP Publication 30 model translocation prediction for class Y compounds

Construction of a T7 Human Lung Cancer cDNA Library

Directory of Open Access Journals (Sweden)

Wentao YUE

2008-10-01

Full Text Available Background and objective Currently, only a limited numbers of tumor markers for non small lung cancer (NSCLC diagnosis, new biomarker, such as serum autoantibody may improve the early detection of lung cancer. Our objective is construction human lung squamous carcinoma and adenocarcinoma T7 phage display cDNA library from the tissues of NSCLC patients. Methods mRNA was isolated from a pool of total RNA extract from NSCLC tissues obtained from 5 adenocarcinomas and 5 squamous carcinomas, and then mRNA was reverse transcribed into double stranded cDNA. After digestion, the cDNA was inserted into T7Select 10-3 vector. The phage display cDNA library was constructed by package reaction in vitro and plate proliferation. Plaque assay and PCR were used to evaluate the library.Results Two T7 phage display cDNA library were established. Plaque assay show the titer of lung squamas carcinoma library was 1.8×106 pfu, and the adenocarcinoma library was 5×106 pfu. The phage titer of the amplified library were 3.2×1010 pfu/mL and 2.5×1010 pfu/mL. PCR amplification of random plaque show insert ratio were 100% (24/24 in adenocarcinoma library and 95.8% in human lung squamas carcinoma library (23/24. Insert range from 300 bp to 1 500 bp. Conclusion Two phage display cDNA library from NSCLC were constructed.

Observation of flow dissipation in 3He-B

International Nuclear Information System (INIS)

Eisenstein, J.P.; Packard, R.E.

1982-01-01

Anomalous dissipation is observed in 3 He-B flowing in a U-tube device. The dissipation is of unknown origin and persists to the lowest measured velocity. The position of this result in the framework of other 3 He-B flow experiments is discussed

Capture of ions by vortex lines in pure 4He and 3He--4He solutions

International Nuclear Information System (INIS)

Ostermeier, R.M.; Glaberson, W.I.

1975-01-01

Measurements of the capture cross section for the negative ion by vortex lines in pure 4 He and a 0.94 percent solution of 3 He in 4 He are presented. A measurable capture of positive ions was not observed in either system. In pure 4 He the departure of the experimental data from the predictions of stochastic theory at lower temperatures is explained in terms of a simple breakdown in that theory, and lifetime effects are shown to be of only secondary importance. The decrease in cross section with decreasing temperature, due essentially to a reduced number density of rotons, is predicted by the results of a Monte Carlo calculation. The cross section in the solution follows stochastic theory to significantly lower temperatures, but at about 1 K there again occurs a marked discrepancy. Measurements of the ion trapping lifetime at low temperatures indicate that it is the finite lifetime that accounts for this discrepancy. Elimination of lifetime effects results in fair agreement between the cross section data in the solution and the predictions of stochastic theory. (11 figures) (auth)

Je-Chun-Jun induced apoptosis of human cervical carcinoma HeLa cells

Institute of Scientific and Technical Information of China (English)

Han-jung CHAE; Kyung-mi PARK; Geun-youn LEE; Gi-seup JEONG; Hyung-rae PARK; Hyung-min KIM; Soo-wan CHAE; Shim-keun YOO; Hyung-ryong KIM

2004-01-01

AIM: To study the mechanism of Je-Chun-Jun (JCJ)-inducing the apoptosis of the human cervical carcinoma,HeLa cells. METHODS: The cell viability was assessed using MTT assay. The optical density was measured at 570 nm. The caspase activity was measured using 50 mmol/L of fluorogenic substrate, AC-DEVD-AMC (caspase3), AC-VEID-AMC (caspase-8) or AC-LEHD-AFC (caspase-9). To confirm the expression of proteins, Western blotting was performed. To detect the characteristic of apoptosis chromatin condensation, HeLa cells were stained with Hoechst 33258 in the presence of JCJ. For the cell cycle analysis, HeLa cells were incubated with Propidium iodide (PI) solution. Fluorescence intensity of cell cycle was measured using flow cytometry system. RESULTS:The loss of viability occurred following the exposure of 10 g/L JCJ. Cells treated with 10 g/L JCJ for 3 d exhibited the apoptotic morphology (brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining) and the reduction of cell volume. Cells incubated with JCJ for 48 h were arrested at the G1 phase of cell cycle and their G1 checkpoint related gene products such as cyclin D1 were transiently decreased. We showed that JCJ induced the p38 MAPK activation in HeLa cells. The p38 MAPK inhibitor, SB203580 protected Hela cells from the JCJ-induced death as well as intervened the JCJ-induced accumulation of cells at the G1 phase. In contrast, MEK1 (-ERK upstream) inhibitor, PD98059 had no effect on HeLa cells. CONCLUSION: JCJ induced cell cycle arrest and apoptosis of HeLa cells through p38 MAPK pathway.

Radiation sensitization by CAPE on human HeLa cells of cervical cancer

International Nuclear Information System (INIS)

Wang Xiaoqiang; Cao Jianping; Fan Saijun; Zun Wei; Huang Xiaofei; Liu Yang; Chen Xialin; Gong Xiaomei; Peng Xiaomei; Zeng Jing

2009-01-01

Objective: To study the radiosensitizing effect of caffic acid phenethyl ester (CAPE) on human cervical cancer HeLa cells. Methods: MTT assay was used to measure the relation between the inhibition effect and CAPE concentrations by CAPE with different concentrations on HeLa cells for 24 hours. HeLa cells were divided into the control and experimental groups, both of which were given 0, 2, 4, 6 and 8 Gy of 60Co γ-irradiation, respectively. The cell clones were counted. Meanwhile HeLa cells were divided into the control, CAPE, irradiation and combination groups. Flow cytometric analysis was adopted to detect the changes of cell cycle distribution induced by CAPE. Results: The inhibition rate of CAPE acting on Hela cells increased with concentrations (F=126. 49 ∼ 3654.88, P 0 ) (1.45 and 1.82 Gy) and the quasi-threshold dose (D q ) (1.89 and 3.21 Gy) of HeLa cells in experimental group decreased comparing with control group, SER was 1.26. Compared with the sole irradiation group, cells in G 2 /M phase of the CAPE group and the sole irradiation group increased (P 2 /M arrest and may be related to the inhibition of the sub-lethal damage repair. (authors)

Specific features of kinetics of He/sup 3/-He/sup 4/ solid solution transformations at superlow temperatures

Energy Technology Data Exchange (ETDEWEB)

Mikheev, V A; Majdanov, V A; Mikhin, N P

1986-06-01

The NMR data on the phase transition kinetics of /sup 3/He-/sup 4/He solid solutions at T=100 mK are considered. Studied are solid helium samples of a molecular volume of 20.55 cm/sup 2//mol with a /sup 3/He content of 0.54%. An unusually long phase transition time is found which is dependent on the prehistory of sample. The spin diffusion of /sup 3/He in the transformated solution concentrated phase is found to be of a quasi-one-dimensional nature with the diffusion coefficient value typical of liquid.

Ab initio study of small He cluster ions Hen+, n=2, 3, 4, 5, and low-lying Rydberg states of He4

International Nuclear Information System (INIS)

Staemmler, V.

1990-01-01

SCF and CEPA calculations are applied to study the structure of small He cluster ions, He n 3 , n=2, 3, 4, 5 and some low-lying Rydberg states of He 4 . The effect of electron correlation upon the equilibrium structures and binding energies is discussed. He 3 + has a linear symmetric equilibrium geometry with a bond length of 2.35 a 0 and a binding energy D e =0.165 eV with respect to He 2 + +He (experimentally: D 0 =0.17 eV which corresponds to D e ≅0.20 eV). He 4 + is a very floppy molecular ion with several energetically very similar geometrical configurations. Our CEPA calculations yield a T-shaped form with a He 3 + centre (R e =2.35 a 0 ) and one inductively bound He atom (4.39 a 0 from the central He atom of He 3 + ) as equilibrium structure. Its binding energy with respect to He 3 + +He is 0.031 eV. A linear symmetric configuration consisting of a He 2 + centre with a bond length of 2.10 a 0 and two inductively bound He atoms (4.20 a 0 from the centre of He 2 + ) is only 0.02-0.03 eV higher in energy. We expect that in larger He cluster ions structures with He 2 + and He 3 + centres and n-2 or n-3 inductively bound He atoms have nearly the same energies. In He 4 a low-lying metastable Rydberg state ( 3 π symmetry for linear He 4 * , 3 B 1 for the T-shaped form) exists which is slightly stronger bound with respect to He 3 * +He than the corresponding ion. (orig.)

3He/4He production ratios by tetrahedral symmetric condensation

International Nuclear Information System (INIS)

Takahashi, Akito

2006-01-01

The present paper treats application of the Electronic Quasi-Particle Expansion Theory (EQPET) model for Tetrahedral Symmetric Condensate (TSC) of H/D mixed systems for Pd host metal. Production ratios of 3 He/ 4 He for multi-body fusion reactions in H/D mixed TSC systems are calculated as a function of H/D mixing ratio. The model is further extended to treat direct nuclear interactions between host-metal nucleus and TSC of pure four protons (or four deuterons), since TSC can become very small (far less than 1 pm radius) charge-neutral pseudo-particle. Results for the case of Ni + 4p/TSC are discussed with Ni + p capture reactions and Ni + 4p fission reactions. (authors)

N-isopropyl-p-iodoamphetamine receptors in normal and cancerous tissue of the human lung

Energy Technology Data Exchange (ETDEWEB)

Tanaka, Eiko; Mishima, Michiaki; Kawakami, Kenzo; Sakai, Naoki; Sugiura, Naoharu; Kuno, Kenshi [Kyoto Univ. (Japan). Dept. of Clinical Physiology; Taniguchi, Takashi [Kyoto Pharmaceutical Univ. (Japan). Dept. of Neurobiology

1993-04-01

N-Isopropyl-p-iodoamphetamine (IMP) receptors in normal human lung tissue were characterized using a radioligand binding assay with iodine-125 IMP as the ligand. Saturation binding studies revealed the presence of two binding sites with dissociation constant (K[sub d]) values of 53[+-]2 and 4687[+-]124 nM and maximum binding capacity (Bmax) values of 7[+-]1 and 133[+-]27 pmol/mg protein (n=5) respectively. The IC[sub 50] values of various amines were as follows: IMP, 9x10[sup -5] M; propranolol, 5x10[sup -4] M; haloperidol, 6x10[sup -4] M; ketamine, 9x10[sup -3] M; dopamine, 1x10[sup -2] M. The IMP receptors of cancerous tissue obtained from human lung also had two binding sites with K[sub d] values of 54[+-]2 and 5277[+-]652 nM and Bmax values of 7[+-]1 and 103[+-]21 pmol/mg protein (n=3) respectively. There was no significant difference in binding parameters between normal and cancerous lung tissue. These results demonstrate the existence of IMP receptors and suggest that cancer does not affect the nature of IMP receptors in human lung tissue. (orig.).

DUAL INHIBITION OF PI3K/AKT AND mTOR SIGNALING IN HUMAN NON-SMALL CELL LUNG CANCER CELLS BY A DIETARY FLAVONOID FISETIN

Science.gov (United States)

Khan, Naghma; Afaq, Farrukh; Khusro, Fatima H.; Adhami, Vaqar Mustafa; Suh, Yewseok; Mukhtar, Hasan

2011-01-01

Lung cancer is one of the most commonly occurring malignancies. It has been reported that mTOR is phosphorylated in lung cancer and its activation was more frequent in tumors with over-expression of PI3K/Akt. Therefore, dual inhibitors of PI3K/Akt and mTOR signaling could be valuable agents for treating lung cancer. In the present study, we show that fisetin, a dietary tetrahydroxyflavone inhibits cell-growth with the concomitant suppression of PI3K/Akt and mTOR signaling in human non-small cell lung cancer (NSCLC) cells. Using autodock 4, we found that fisetin physically interacts with the mTOR complex at two sites. Fisetin treatment was also found to reduce the formation of A549 cell colonies in a dose-dependent manner. Treatment of cells with fisetin caused decrease in the protein expression of PI3K (p85 and p110), inhibition of phosphorylation of Akt, mTOR, p70S6K1, eIF-4E and 4E-BP1. Fisetin-treated cells also exhibited dose-dependent inhibition of the constituents of mTOR signaling complex like Rictor, Raptor, GβL and PRAS40. There was increase in the phosphorylation of AMPKα and decrease in the phosphorylation of TSC2 on treatment of cells with fisetin. We also found that treatment of cells with mTOR inhibitor rapamycin and mTOR-siRNA caused decrease in phosphorylation of mTOR and its target proteins which were further downregulated on treatment with fisetin, suggesting that these effects are mediated in part, through mTOR signaling. Our results show that fisetin suppressed PI3K/Akt and mTOR signaling in NSCLC cells and thus, could be developed as a chemotherapeutic agent against human lung cancer. PMID:21618507

6 Ma age of carving Westernmost Grand Canyon: Reconciling geologic data with combined AFT, (U-Th)/He, and 4He/3He thermochronologic data

Science.gov (United States)

Winn, Carmen; Karlstrom, Karl E.; Shuster, David L.; Kelley, Shari; Fox, Matthew

2017-09-01

Conflicting hypotheses about the timing of carving of the Grand Canyon involve either a 70 Ma (;old;) or conflict with these lines of evidence, but are reconciled in this paper via the integration of three methods of analyses on the same sample: apatite (U-Th)/He ages (AHe), 4He/3He thermochronometry (4He/3He), and apatite fission-track ages and lengths (AFT). HeFTy software was used to generate time-temperature (t-T) paths that predict all new and published 4He/3He, AHe, and AFT data to within assumed uncertainties. These t-T paths show cooling from ∼100 °C to 40-60 °C in the Laramide (70-50 Ma), long-term residence at 40-60 °C in the mid-Tertiary (50-10 Ma), and cooling to near-surface temperatures after 10 Ma, and thus support young incision of the westernmost Grand Canyon. A subset of AHe data, when interpreted alone (i.e. without 4He/3He or AFT data), are better predicted by t-T paths that cool to surface temperatures during the Laramide, consistent with an ;old; Grand Canyon. However, the combined AFT, AHe, and 4He/3He analysis of a key sample from Separation Canyon can only be reconciled by a ;young; Canyon. Additional new AFT (5 samples) and AHe data (3 samples) in several locations along the canyon corridor also support a ;young; Canyon. This inconsistency, which mimics the overall controversy of the age of the Grand Canyon, is reconciled here by optimizing cooling paths so they are most consistent with multiple thermochronometers from the same rocks. To do this, we adjusted model parameters and uncertainties to account for uncertainty in the rate of radiation damage annealing in these apatites during sedimentary burial and the resulting variations in He retentivity. In westernmost Grand Canyon, peak burial conditions (temperature and duration) during the Laramide were likely insufficient to fully anneal radiation damage that accumulated during prolonged, near-surface residence since the Proterozoic. We conclude that application of multiple

Production of d, t, 3He, anti d, anti t and anti 3He by 200 GeV protons

International Nuclear Information System (INIS)

Bozzoli, W.; Giacomelli, G.; Rimondi, F.; Zylberajch, S.; Lesquoy, E.; Meunier, R.; Moscoso, L.; Muller, A.; Bussiere, A.

1978-01-01

Data are presented on the yields of d, t, 3 He, anti d, anti t, and anti 3 He with laboratory momenta between 12 and 37 GeV/c produced by 200 GeV protons on beryllium and aluminium. The production yield of nuclei depends significantly on the target nucleus, while the anti d production is independent of target material. The mass dependence of the production cross section is exponential for both nuclei and antinuclei

MiR-26a enhances invasive capacity by suppressing GSK3β in human lung cancer cells

Energy Technology Data Exchange (ETDEWEB)

Lin, Gaoyang; Liu, Boning [Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052 (China); Meng, Zhaowei [Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052 (China); Liu, Yunde [School of Laboratory Medicine, Tianjin Medical University, Tianjin 300052 (China); Li, Xuebing [Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052 (China); Wu, Xiang [Core Facility Center, Tianjin Medical University General Hospital, Tianjin 300052 (China); Zhou, Qinghua [Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052 (China); Xu, Ke, E-mail: ke_xu@hotmail.com [Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052 (China)

2017-03-15

Lung cancer is the common cause of death from cancer, and most lung cancer patients die of metastasis. MicroRNAs (miRNAs) function as either oncogenes or tumor suppressors, playing crucial role not only in tumorigenesis, but also in tumor invasion and metastasis. There are several studies showed that miR-26a is involved in carcinogenesis, however, its role in tumor metastasis need to be elucidated. In this study, we showed that ectopic expression of miR-26a enhanced migration and invasion of lung cancer cells. Glycogen synthase kinase-3β (GSK3β) was identified as a direct target of miR-26a. GSK3β expression negatively correlated with miR-26a expression in lung cancer tissues. Silencing of GSK3β achieved similar effect as miR-26a over-expression; over-expression of GSK3β reversed the enhanced effect of miR-26a on lung cancer cell migration and invasion. Further study indicated that miR-26a increased β-catenin expression and nuclear translocation. C-myc and cyclin D1, the downstream genes of β-catenin, were also up-regulated by miR-26a. Furthermore, xenograft study showed that miR-26a promoted lung cancer cell growth in vivo, and suppressed GSK3β expression. Collectively, our results demonstrated that miR-26a enhanced metastatic potential of lung cancer cells via activation of β-catenin pathway by targeting GSK3β, suggesting the potential applicability of miR-26a as a target for cancer treatment. - Highlights: • miR-26a enhances migration and invasion of lung cancer cells. • GSK3β is identified as a direct target of miR-26a. • miR-26a activates β-catenin pathway by targeting GSK3β. • miR-26a promotes lung cancer cell growth in vivo.

MiR-26a enhances invasive capacity by suppressing GSK3β in human lung cancer cells

International Nuclear Information System (INIS)

Lin, Gaoyang; Liu, Boning; Meng, Zhaowei; Liu, Yunde; Li, Xuebing; Wu, Xiang; Zhou, Qinghua; Xu, Ke

2017-01-01

Lung cancer is the common cause of death from cancer, and most lung cancer patients die of metastasis. MicroRNAs (miRNAs) function as either oncogenes or tumor suppressors, playing crucial role not only in tumorigenesis, but also in tumor invasion and metastasis. There are several studies showed that miR-26a is involved in carcinogenesis, however, its role in tumor metastasis need to be elucidated. In this study, we showed that ectopic expression of miR-26a enhanced migration and invasion of lung cancer cells. Glycogen synthase kinase-3β (GSK3β) was identified as a direct target of miR-26a. GSK3β expression negatively correlated with miR-26a expression in lung cancer tissues. Silencing of GSK3β achieved similar effect as miR-26a over-expression; over-expression of GSK3β reversed the enhanced effect of miR-26a on lung cancer cell migration and invasion. Further study indicated that miR-26a increased β-catenin expression and nuclear translocation. C-myc and cyclin D1, the downstream genes of β-catenin, were also up-regulated by miR-26a. Furthermore, xenograft study showed that miR-26a promoted lung cancer cell growth in vivo, and suppressed GSK3β expression. Collectively, our results demonstrated that miR-26a enhanced metastatic potential of lung cancer cells via activation of β-catenin pathway by targeting GSK3β, suggesting the potential applicability of miR-26a as a target for cancer treatment. - Highlights: • miR-26a enhances migration and invasion of lung cancer cells. • GSK3β is identified as a direct target of miR-26a. • miR-26a activates β-catenin pathway by targeting GSK3β. • miR-26a promotes lung cancer cell growth in vivo.

Coherent photoproduction of π+ on 3He in the (3,3) resonance region

International Nuclear Information System (INIS)

Boyard, J.-L.

1975-01-01

An experimental study of the 3 He(γ,π + ) 3 H reaction has been performed in the (3,3) resonance region with a bremsstrahlung photon beam and a liquid 3 He target. A magnetic spectrometer followed by a wire chamber analyzed the momentum of the outgoing pions and defined their emission angle. The tritons were detected in coincidence with the pions by two methods: a telescope of thin plastic scintillators below 40MeV; or a magnetic spectrometer followed by a series of plastic scintillators for higher energies. The experiment shows two main features: at a fixed four-momentum transfer q 2 , the resonance is shifted towards lower energies, this shift increasing with q 2 ; at a fixed angle, but variable q 2 , the minimum of the charge form factor observed in the elastic scattering of electrons on 3 He does not appear. Calculations explain partly these two effects [fr

POLARIZED-LIGHT EMISSION IN KEV HE2++NA(3S) COLLISIONS

NARCIS (Netherlands)

BODUCH, P; VANBUCHEM, J; BLIEK, FW; HOEKSTRA, R; MORGENSTERN, R; OLSON, RE

1995-01-01

We investigated l and m-distributions of He II (n = 4) states formed during 2-13.3 keV amu(-1) He2+ + Na(3s) collisions by measuring profiles and the linear polarization of the He II (4 --> 3) emission. We found that at these energies capture into He+(4f) dominates over capture into other He+(4l)

Endostar, a recombined humanized endostatin, enhances the radioresponse for human nasopharyngeal carcinoma and human lung adenocarcinoma xenografts in mice

International Nuclear Information System (INIS)

Wen Qinglian; Meng Maobin; Tu Lingli; Jia Li; Zhou Lin; Xu Yong; Lu You; Yang Bo

2009-01-01

The purpose of this paper is to determine the efficacy of combining radiation therapy with endostar, a recombined humanized endostatin, in human nasopharyngeal carcinoma and human lung adenocarcinoma xenografts. Tumor xenografts were established in the hind limb of male athymic nude mice (BALB/c-nu) by subcutaneous transplantation. The tumor-bearing mice were assigned into four treatment groups: sham therapy (control), endostar (20 mg/kg, once daily for 10 days), radiation therapy (6 Gray per day to 30 Gray, once a day for 1 week), and endostar plus radiation therapy (combination). The experiment was repeated and mice were killed at days 3, 6, and 10 after initiation therapy, and the tumor tissues and blood samples were collected to analyze the kinetics of antitumor, antiangiogenesis, and antivascularization responses of different therapies. In human nasopharyngeal carcinoma and human lung adenocarcinoma xenografts, endostar significantly enhanced the effects of tumor growth inhibition, endothelial cell and tumor cell apoptosis induction, and improved tumor cell hypoxia of radiation therapy. Histological analyses demonstrated that endostar plus radiation also induced a significant reduction in microvascular density, microvascular area, and vascular endothelial growth factor and matrix metalloproteinase-2 expression compared with radiation and endostar alone respectively. We concluded that endostar significantly sensitized the function of radiation in antitumor and antiangiogenesis in human nasopharyngeal carcinoma and human lung adenocarcinoma xenografts by increasing the apoptosis of the endothelial cell and tumor cell, improving the hypoxia of the tumor cell, and changing the proangiogenic factors. These data provided a rational basis for clinical practice of this multimodality therapy. (author)

Gene Expression Analysis to Assess the Relevance of Rodent Models to Human Lung Injury.

Science.gov (United States)

Sweeney, Timothy E; Lofgren, Shane; Khatri, Purvesh; Rogers, Angela J

2017-08-01

The relevance of animal models to human diseases is an area of intense scientific debate. The degree to which mouse models of lung injury recapitulate human lung injury has never been assessed. Integrating data from both human and animal expression studies allows for increased statistical power and identification of conserved differential gene expression across organisms and conditions. We sought comprehensive integration of gene expression data in experimental acute lung injury (ALI) in rodents compared with humans. We performed two separate gene expression multicohort analyses to determine differential gene expression in experimental animal and human lung injury. We used correlational and pathway analyses combined with external in vitro gene expression data to identify both potential drivers of underlying inflammation and therapeutic drug candidates. We identified 21 animal lung tissue datasets and three human lung injury bronchoalveolar lavage datasets. We show that the metasignatures of animal and human experimental ALI are significantly correlated despite these widely varying experimental conditions. The gene expression changes among mice and rats across diverse injury models (ozone, ventilator-induced lung injury, LPS) are significantly correlated with human models of lung injury (Pearson r = 0.33-0.45, P human lung injury. Predicted therapeutic targets, peptide ligand signatures, and pathway analyses are also all highly overlapping. Gene expression changes are similar in animal and human experimental ALI, and provide several physiologic and therapeutic insights to the disease.

Teaching basic lung isolation skills on human anatomy simulator: attainment and retention of lung isolation skills.

Science.gov (United States)

Latif, Rana K; VanHorne, Edgar M; Kandadai, Sunitha Kanchi; Bautista, Alexander F; Neamtu, Aurel; Wadhwa, Anupama; Carter, Mary B; Ziegler, Craig H; Memon, Mohammed Faisal; Akça, Ozan

2016-01-20

Lung isolation skills, such as correct insertion of double lumen endobronchial tube and bronchial blocker, are essential in anesthesia training; however, how to teach novices these skills is underexplored. Our aims were to determine (1) if novices can be trained to a basic proficiency level of lung isolation skills, (2) whether video-didactic and simulation-based trainings are comparable in teaching lung isolation basic skills, and (3) whether novice learners' lung isolation skills decay over time without practice. First, five board certified anesthesiologist with experience of more than 100 successful lung isolations were tested on Human Airway Anatomy Simulator (HAAS) to establish Expert proficiency skill level. Thirty senior medical students, who were naive to bronchoscopy and lung isolation techniques (Novice) were randomized to video-didactic and simulation-based trainings to learn lung isolation skills. Before and after training, Novices' performances were scored for correct placement using pass/fail scoring and a 5-point Global Rating Scale (GRS); and time of insertion was recorded. Fourteen novices were retested 2 months later to assess skill decay. Experts' and novices' double lumen endobronchial tube and bronchial blocker passing rates showed similar success rates after training (P >0.99). There were no differences between the video-didactic and simulation-based methods. Novices' time of insertion decayed within 2 months without practice. Novices could be trained to basic skill proficiency level of lung isolation. Video-didactic and simulation-based methods we utilized were found equally successful in training novices for lung isolation skills. Acquired skills partially decayed without practice.

Development of 3He-BOCA power ramping facility, 1

International Nuclear Information System (INIS)

Nakata, Hirokatsu; Ishii, Tadahiko; Itoh, Haruhiko; Abe, Hiroshi; Nakazaki, Chozaburo

1979-11-01

Development of a He-3 power controlled boiling water capsule, 3 He-BOCS, for LWR fuels power ramping test in JMTR has been carried out since 1978 on a five-year program; in the reactor, irradiation tests of various fuels and structual materials have been made since 1969. Using stagnant-pressurized water as a thermal medium, the capsule provides pressure and temperature conditions similar to those in LWRs. Heat generation of a fuel pin can be controlled by a He-3 gas screen surrounding the capsule. The facility is capable of testing numbers of both fresh and irradiated fuel pins under LWR operating conditions for power ramping and cycling. After explaining the operating priciples of 3 He-BOCA and the development program, the following are described: the results of preliminary out-of-pile test on heat conductive characteristics of the capsule and a conceptual design of the 3 He-BOCA for power ramping of a short fuel pin from 250 W/cm to 500 W/cm under BWR conditions. (author)

A Lentinus edodes polysaccharide induces mitochondrial-mediated apoptosis in human cervical carcinoma HeLa cells.

Science.gov (United States)

Ya, Guowei

2017-10-01

In this study, a homogeneous polysaccharide (LEP1) with an average molecular weight of 53kDa was successfully purified from the fruiting bodies of Lentinus edodes and its anticancer efficacy on human cervical carcinoma HeLa cells in vitro and associated possible molecular mechanism were also evaluated. MTT assay showed that LEP1 exhibited a dose-dependent inhibitory effect on the proliferation of HeLa cells and caused apoptotic death. Our present findings provided the first evidence that LEP1 induced the apoptosis of HeLa cells via a mitochondria dependent pathway, as indicated by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δym), the release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly (ADP-ribose) polymerase (PARP) in HeLa cells. These combined results unequivocally indicated that the involvement of mitochondria-mediated signaling pathway in LEP1-induced apoptosis and strongly provided experimental evidence for the use of LEP1 as a potential therapeutic agent in the prevention and treatment of human cervical carcinoma. Copyright © 2017 Elsevier B.V. All rights reserved.

Expectation values of the e+He(3Se) system

International Nuclear Information System (INIS)

Mitroy, J.

2005-01-01

Close to converged energies and expectation values for e + He( 3 S e ) are computed using a ground state wave function consisting of 1500 explicitly correlated Gaussians. The best estimate of the e + He( 3 S e ) energy was -2.250 595 08 hartree, which has a binding energy of 0.000 595 08 hartree against dissociation into Ps+He + . The 2γ annihilation rate for the spin doublet state was 5.713x10 9 s -1 . The estimated annihilation rate with the core He + electron was 2.506x10 6 s -1 . The derived enhancement factor for annihilation with the He + (1s) core was 2.29, just over 10% smaller than the enhancement factor derived from analyses of annihilation during e + -He + scattering. The diffuse nature of the wave function, with well separated He + and Ps subsystems, is demonstarted to be on the threshold of satisfying the formal criteria that define a quantum halo state

The detection, diagnosis and therapy of human lung cancer

International Nuclear Information System (INIS)

1978-01-01

The Cancergram covers clinical aspects of cancers of the lung and tracheo-bronchial tree, i.e., the lower respiratory tract. This includes primary lung cancer in both early and advanced disease status. The topic includes clinically relevant aspects of the prevention, detection, diagnosis, evaluation, and therapy of lung cancer. Certain aspects of metastatic lung disease treatment or therapy which involve aspects of interest to primary lung cancer are included. With certain exceptions, general pre-clinical or animal studies not directly related to the primary human disease are excluded

3He and chlorofluorocarbons (CFC) in the Southern Ocean

International Nuclear Information System (INIS)

Jean-Baptiste, P.; Jamous, D.; Mantisi, F.; Memery, L.; Universite Paris 6

1991-01-01

The distribution of 3 He across the Southern Ocean is depicted on the basis of a meridional section between Antarctica and South Africa measured during the INDIGO-3 survey (1988). A core of δ 3 He values above 10% is observed south of the Polar Front, associated with very low CFC concentrations. This 3 He enriched layer is documented from the GEOSECS and INDIGO 3 He data in the Southern Ocean. It is found at a density level around θ σ =27.8 in all the waters close to Antarctica (i.e. south of 50 degS). Its zonal distribution suggests that it is likely that it originates from the central/eastern Pacific. Hence, it provides an indication of the deep Pacific waters in the Antarctic Circumpolar Current, which are not easily detectable from the standard hydrographic parameters. (author). 19 refs.; 8 figs

Analysis of the reaction 3He + p → p + p + d at 2,5 GeV/c 3He nucleus momentum

International Nuclear Information System (INIS)

Blinov, A.V.; Chuvilo, I.V.; Drobot, V.V.

1984-01-01

The experimental data on the reaction 3 He+p → p+p+d obtained by the exposition of an 80 cm liquid hydrogen bubble chamber to the 3 He nucleus beam at 2.5 GeV/c momentum are considered. The angular, momentum and invariant mass distributions, which have been measured, are compared with theoretical calculations performed using the pole model. On the whole a satisfactory agreement between theoretical and experimental data is found in the kinematical region, where pd- rescattering effects are negligible. It is concluded, that 3 He nuclear wave function calculated by means of nonrelativistic potential models falls too sharply with the spectator momenta as compared with the data

DZNep, inhibitor of S-adenosylhomocysteine hydrolase, down-regulates expression of SETDB1 H3K9me3 HMTase in human lung cancer cells.

Science.gov (United States)

Lee, Ju-Kyung; Kim, Keun-Cheol

2013-09-06

3-Deazaneplanocin A (DZNep), an epigenetic anticancer drug, leads to the indirect suppression of S-adenosyl methionine-dependent cellular methylations by inhibiting S-adenosyl homocystein (AdoHcy) hydrolase. Although it is well known that DZNep targets the degradation of EZH2 protein, H3K27me3 HMTase, there are still uncertainties about the regulation of other types of HMTases during cell death. In this study, we describe that SETDB1 gene expression was regulated by DZNep treatment in human lung cancer cells. We confirm that DZNep induced growth inhibition and increased the dead cell population of lung cancer cells. DZNep treatment affected histone methylations, including H3K27me3 and H3K9me3, but not H3K4me3. Reduced levels of H3K27me3 and H3K9me3 were related with the decreased EZH2 and SETDB1 proteins. Real time PCR analysis showed that SETDB1 gene expression was decreased by DZNep treatment, but no effect was observed for EZH2 gene expression. We cloned the promoter region of SETDB1 and SUV39H1 genes, and performed luciferase assays. The promoter activity of SETDB1 gene was down regulated by DZNep treatment, whereas no effect on SUV39H1 promoter activity was observed. In conclusion, we suggest that DZNep regulates not only on H3K27me3 HMTase EZH2, but also H3K9 HMTase SETDB1 gene expression at the transcription level, implicating that the mechanism of action of DZNep targets multiple HMTases during the death of lung cancer cells. Copyright © 2013 Elsevier Inc. All rights reserved.

E-cigarette smoke damages DNA and reduces repair activity in mouse lung, heart, and bladder as well as in human lung and bladder cells

OpenAIRE

Lee, Hyun-Wook; Park, Sung-Hyun; Weng, Mao-wen; Wang, Hsiang-Tsui; Huang, William C.; Lepor, Herbert; Wu, Xue-Ru; Chen, Lung-Chi; Tang, Moon-shong

2018-01-01

Significance E-cigarette smoke (ECS) delivers nicotine through aerosols without burning tobacco. ECS is promoted as noncarcinogenic. We found that ECS induces DNA damage in mouse lung, bladder, and heart and reduces DNA-repair functions and proteins in lung. Nicotine and its nitrosation product 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone can cause the same effects as ECS and enhance mutations and tumorigenic cell transformation in cultured human lung and bladder cells. These results indica...

Technic and economic viability study on exploitation of lunar 3He resource

International Nuclear Information System (INIS)

Deng Baiquan

1995-01-01

From the energetics point of view, the technic and economic viability study on exploitation of lunar 3 He for fuelling the fusion reactor burning D- 3 He has been carried out. This study is divided into the following sections: analysis of solar wind parameters and estimation of potential quantity 3 He in the lunar regolith, the cost evaluation of mining He of lunar soil; the energy cost calculation of He extraction by vacuum heating degassing during lunar day, the cost calculation of cryogenic isotopic separation 3 He/ 4 He during the lunar night, the energy cost for earth/moon transportation of liquid 3 He, the energy payback calculation of fusion power burning 3 He based lunar source, and finally the comparison of the energy multiplication with that for 235 U production of nuclear fuel and for coal mining. The comparisons of cost of electricity between D- 3 He and D-T fuel cycle for different reactor types have been discussed

Spin Superfluidity and Magnone BEC in He-3

Science.gov (United States)

Bunkov, Yury

2011-03-01

The spin superfluidity -- superfluidity in the magnetic subsystem of a condensed matter -- is manifested as the spontaneous phase-coherent precession of spins first discovered in 1984 in 3 He-B. This superfluid current of spins -- spin supercurrent -- is one more representative of superfluid currents known or discussed in other systems, such as the superfluid current of mass and atoms in superfluid 4 He; superfluid current of electric charge in superconductors; superfluid current of hypercharge in Standard Model of particle physics; superfluid baryonic current and current of chiral charge in quark matter; etc. Spin superfluidity can be described in terms of the Bose condensation of spin waves -- magnons. We discuss different states of magnon superfluidity with different types of spin-orbit coupling: in bulk 3 He-B; magnetically traped `` Q -balls'' at very low temperatures; in 3 He-A and 3 He-B immerged in deformed aerogel; etc. Some effects in normal 3 He can also be treated as a magnetic BEC of fermi liquid. A very similar phenomena can be observed also in a magnetic systems with dinamical frequensy shift, like MnC03 . We will discuss the main experimental signatures of magnons superfluidity: (i) spin supercurrent, which transports the magnetization on a macroscopic distance more than 1 cm long; (ii) spin current Josephson effect which shows interference between two condensates; (iii) spin current vortex -- a topological defect which is an analog of a quantized vortex in superfluids, of an Abrikosov vortex in superconductors, and cosmic strings in relativistic theories; (iv) Goldstone modes related to the broken U (1) symmetry -- phonons in the spin-superfluid magnon gas; etc. For recent review see Yu. M. Bunkov and G. E. Volovik J. Phys. Cond. Matter. 22, 164210 (2010) This work is partly supported by the Ministry of Education and Science of the Russian Federation (contract N 02.740.11.5217).

Second sound, osmotic pressure, and Fermi-liquid parameters in 3He-4He solutions

International Nuclear Information System (INIS)

Corruccini, L.R.

1984-01-01

Second-sound velocities and osmotic pressures are analyzed to obtain the first experimental values for the Landau compressibility parameter F 0 /sup s/ in 3 He- 4 He solutions. Data are presented as a function of pressure and 3 He concentration, and are compared to theoretical predictions. The square of the second-sound velocity at finite temperature is found to be accurately proportional to the internal energy of a perfect Fermi gas. Using inertial effective masses given by the Landau-Pomeranchuk theory, the square of the velocity is found to separate into two parts: a temperature-dependent part characterized completely by ideal Fermi-gas behavior and a temperature-independent part containing all the Fermi-liquid corrections. This is related to a similar separation found in the osmotic pressure

3He electron scattering sum rules

International Nuclear Information System (INIS)

Kim, Y.E.; Tornow, V.

1982-01-01

Electron scattering sum rules for 3 He are derived with a realistic ground-state wave function. The theoretical results are compared with the experimentally measured integrated cross sections. (author)

Sex-specific differences in hyperoxic lung injury in mice: Implications for acute and chronic lung disease in humans

Energy Technology Data Exchange (ETDEWEB)

Lingappan, Krithika, E-mail: lingappa@bcm.edu [Department of Pediatrics, Section of Neonatology, Texas Children' s Hospital, Baylor College of Medicine, 1102 Bates Avenue, MC: FC530.01, Houston, TX 77030 (United States); Jiang, Weiwu; Wang, Lihua; Couroucli, Xanthi I. [Department of Pediatrics, Section of Neonatology, Texas Children' s Hospital, Baylor College of Medicine, 1102 Bates Avenue, MC: FC530.01, Houston, TX 77030 (United States); Barrios, Roberto [Department of Pathology and Genomic Medicine, The Methodist Hospital Physician Organization, 6565 Fannin Street, Suite M227, Houston, TX 77030 (United States); Moorthy, Bhagavatula [Department of Pediatrics, Section of Neonatology, Texas Children' s Hospital, Baylor College of Medicine, 1102 Bates Avenue, MC: FC530.01, Houston, TX 77030 (United States)

2013-10-15

Sex-specific differences in pulmonary morbidity in humans are well documented. Hyperoxia contributes to lung injury in experimental animals and humans. The mechanisms responsible for sex differences in the susceptibility towards hyperoxic lung injury remain largely unknown. In this investigation, we tested the hypothesis that mice will display sex-specific differences in hyperoxic lung injury. Eight week-old male and female mice (C57BL/6J) were exposed to 72 h of hyperoxia (FiO{sub 2} > 0.95). After exposure to hyperoxia, lung injury, levels of 8-iso-prostaglandin F{sub 2} alpha (8-iso-PGF 2α) (LC–MS/MS), apoptosis (TUNEL) and inflammatory markers (suspension bead array) were determined. Cytochrome P450 (CYP)1A expression in the lung was assessed using immunohistochemistry and western blotting. After exposure to hyperoxia, males showed greater lung injury, neutrophil infiltration and apoptosis, compared to air-breathing controls than females. Pulmonary 8-iso-PGF 2α levels were higher in males than females after hyperoxia exposure. Sexually dimorphic increases in levels of IL-6 (F > M) and VEGF (M > F) in the lungs were also observed. CYP1A1 expression in the lung was higher in female mice compared to males under hyperoxic conditions. Overall, our results support the hypothesis that male mice are more susceptible than females to hyperoxic lung injury and that differences in inflammatory and oxidative stress markers contribute to these sex-specific dimorphic effects. In conclusion, this paper describes the establishment of an animal model that shows sex differences in hyperoxic lung injury in a temporal manner and thus has important implications for lung diseases mediated by hyperoxia in humans. - Highlights: • Male mice were more susceptible to hyperoxic lung injury than females. • Sex differences in inflammatory markers were observed. • CYP1A expression was higher in females after hyperoxia exposure.

Sex-specific differences in hyperoxic lung injury in mice: Implications for acute and chronic lung disease in humans

International Nuclear Information System (INIS)

Lingappan, Krithika; Jiang, Weiwu; Wang, Lihua; Couroucli, Xanthi I.; Barrios, Roberto; Moorthy, Bhagavatula

2013-01-01

Sex-specific differences in pulmonary morbidity in humans are well documented. Hyperoxia contributes to lung injury in experimental animals and humans. The mechanisms responsible for sex differences in the susceptibility towards hyperoxic lung injury remain largely unknown. In this investigation, we tested the hypothesis that mice will display sex-specific differences in hyperoxic lung injury. Eight week-old male and female mice (C57BL/6J) were exposed to 72 h of hyperoxia (FiO 2 > 0.95). After exposure to hyperoxia, lung injury, levels of 8-iso-prostaglandin F 2 alpha (8-iso-PGF 2α) (LC–MS/MS), apoptosis (TUNEL) and inflammatory markers (suspension bead array) were determined. Cytochrome P450 (CYP)1A expression in the lung was assessed using immunohistochemistry and western blotting. After exposure to hyperoxia, males showed greater lung injury, neutrophil infiltration and apoptosis, compared to air-breathing controls than females. Pulmonary 8-iso-PGF 2α levels were higher in males than females after hyperoxia exposure. Sexually dimorphic increases in levels of IL-6 (F > M) and VEGF (M > F) in the lungs were also observed. CYP1A1 expression in the lung was higher in female mice compared to males under hyperoxic conditions. Overall, our results support the hypothesis that male mice are more susceptible than females to hyperoxic lung injury and that differences in inflammatory and oxidative stress markers contribute to these sex-specific dimorphic effects. In conclusion, this paper describes the establishment of an animal model that shows sex differences in hyperoxic lung injury in a temporal manner and thus has important implications for lung diseases mediated by hyperoxia in humans. - Highlights: • Male mice were more susceptible to hyperoxic lung injury than females. • Sex differences in inflammatory markers were observed. • CYP1A expression was higher in females after hyperoxia exposure

Lowest vibrational states of 4He3He+: Non-Born-Oppenheimer calculations

International Nuclear Information System (INIS)

Stanke, Monika; Bubin, Sergiy; Kedziera, Dariusz; Molski, Marcin; Adamowicz, Ludwik

2007-01-01

Very accurate quantum mechanical calculations of the first five vibrational states of the 4 He 3 He + molecular ion are reported. The calculations have been performed explicitly including the coupling of the electronic and nuclear motions [i.e., without assuming the Born-Oppenheimer (BO) approximation]. The nonrelativistic non-BO wave functions were used to calculate the α 2 relativistic mass velocity, Darwin, and spin-spin interaction corrections. For the lowest vibrational transition, whose experimental energy is established with high precision, the calculated and the experimental results differ by only 0.16 cm -1

Development of polarized {sup 3}He filter for polarized neutron experiment

Energy Technology Data Exchange (ETDEWEB)

Sakai, K.; Sato, H.; Yoshimi, A.; Asahi, K. [Tokyo Inst. of Tech. (Japan). Faculty of Science; Masuda, Y.; Muto, S.; Ishimoto, S.; Morimoto, K.

1996-08-01

A high-pressure polarized {sup 3}He gas cell, pumped with two diode lasers, has been developed at KEK for use as a polarizer and a spin analyzer for low energy neutrons. The polarization attained of {sup 3}He was determined through the measurement of the transmission of the unpolarized neutrons through the {sup 3}He cell. So far we obtained P{sub He}=18% at 10 atm and P{sub He}=12% at 20 atm. (author)

Study of the {sup 6}Li + p → {sup 3}He + {sup 4}He reaction in inverse kinematics

Energy Technology Data Exchange (ETDEWEB)

Betsou, C.; Pakou, A.; Aslanoglou, X.; Nicolis, N.G.; Sgouros, O.; Soukeras, V. [The University of Ioannina, Department of Physics and HINP, Ioannina (Greece); Cappuzzello, F. [Laboratori Nazionali del Sud, INFN, Catania (Italy); Universita di Catania, Dipartimento di Fisica e Astronomia, Catania (Italy); Acosta, L. [Universidad Nacional Autonoma de Mexico, Instituto de Fisica, Mexico Distrito Federal (Mexico); INFN, Catania (Italy); Agodi, C.; Carbone, D.; Cavallaro, M.; Di Pietro, A.; Fernandez-Garcia, J.P.; Figuera, P.; Fisichella, M. [Laboratori Nazionali del Sud, INFN, Catania (Italy); Foti, A. [Universita di Catania, Dipartimento di Fisica e Astronomia, Catania (Italy); INFN, Catania (Italy); Keeley, N. [National Centre for Nuclear Research, Otwock (Poland); Marquinez-Duran, G.; Martel, I. [Universidad de Huelva, Departamento de Fisica Aplicada, Huelva (Spain); Mazzocco, M.; Strano, E.; Torresi, D. [Universita di Padova, Departimento di Fisica e Astronomia, Padova (Italy); INFN, Padova (Italy); Pierroutsakou, D. [INFN, Napoli (Italy); Rusek, K. [University of Warsaw, Heavy Ion Laboratory, Warsaw (Poland); Stiliaris, E. [University of Athens, Institute of Accelerating Systems and Applications and Department of Physics, Athens (Greece)

2015-07-15

Angular distribution measurements were performed for the {sup 6}Li + p → {sup 3}He + {sup 4}He reaction in inverse kinematics at incident energies of 2.7, 3.3, 4.2 and 4.8 MeV/u. The detection of both recoils ({sup 3}He and {sup 4}He) over the laboratory angle range θ{sub lab} = 16 {sup circle} to 34 {sup circle} allowed the determination of the angular distribution over a wide angular range in the center-of-mass frame (θ{sub c.m.} ∝ 40 {sup circle} to 140 {sup circle}). The results clarify inconsistencies between existing data sets and are consistent with compound nucleus model calculations. (orig.)

Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells.

Science.gov (United States)

Wu, Liang; Zhang, Xiaolong; Zhao, Zhikun; Wang, Ling; Li, Bo; Li, Guibo; Dean, Michael; Yu, Qichao; Wang, Yanhui; Lin, Xinxin; Rao, Weijian; Mei, Zhanlong; Li, Yang; Jiang, Runze; Yang, Huan; Li, Fuqiang; Xie, Guoyun; Xu, Liqin; Wu, Kui; Zhang, Jie; Chen, Jianghao; Wang, Ting; Kristiansen, Karsten; Zhang, Xiuqing; Li, Yingrui; Yang, Huanming; Wang, Jian; Hou, Yong; Xu, Xun

2015-01-01

Viral infection causes multiple forms of human cancer, and HPV infection is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. HeLa is a well characterized HPV+ cervical cancer cell line. We developed a new high throughput platform to prepare single-cell RNA on a nanoliter scale based on a customized microwell chip. Using this method, we successfully amplified full-length transcripts of 669 single HeLa S3 cells and 40 of them were randomly selected to perform single-cell RNA sequencing. Based on these data, we obtained a comprehensive understanding of the heterogeneity of HeLa S3 cells in gene expression, alternative splicing and fusions. Furthermore, we identified a high diversity of HPV-18 expression and splicing at the single-cell level. By co-expression analysis we identified 283 E6, E7 co-regulated genes, including CDC25, PCNA, PLK4, BUB1B and IRF1 known to interact with HPV viral proteins. Our results reveal the heterogeneity of a virus-infected cell line. It not only provides a transcriptome characterization of HeLa S3 cells at the single cell level, but is a demonstration of the power of single cell RNA-seq analysis of virally infected cells and cancers.

Pionic annihilation of antiprotons stopped on 3He

International Nuclear Information System (INIS)

Bendiscioli, G.; Filippini, V.; Rotondi, A.; Salvini, P.; Venaglioni, A.; Bossolasco, S.; Bussa, M.P.; Busso, L.; Fava, L.; Ferrero, L.; Maggiora, A.; Panzieri, D.; Piragino, G.; Piragino, R.; Tosello, F.; Batusov, Yu.A.; Falomkin, I.V.; Pontecorvo, G.B.; Rozhdestvensky, A.M.; Sapozhnikov, M.G.; Tretyak, V.I.; Guaraldo, C.; Haatuft, A.; Halsteinslid, A.; Myklebost, K.; Olsen, J.M.; Zenoni, A.

1990-01-01

The pionic annihilation of antiprotons stopped on 3 He nuclei in a self-shunted streamer chamber exposed to the antiproton beam of LEAR is studied. The data concern charged-particle multiplicity distributions, branching ratios for different final states, the probability of final-state interaction, π - , π + , p and d momentum spectra, like and unlike pion angular correlations, π and other charged-particle angular correlations. The comparison of the 3 He data with those obtained on 1 H, 2 H and 4 He does not reveal relevant effects due to the increase of the nucleon number; the small differences can be seen as due to a weak final-state interaction. (orig.)

4He(γ,n)3He photodisintegration and the properties of O+-resonance of 4He by the resonating group method

International Nuclear Information System (INIS)

Filippov, G.F.; Vasilevsky, V.S.; Kovalenko, T.P.

1984-01-01

The reaction of 4 He electrodisintegration at small momenta transfered to the nucleus by electrons is considered on the basis of an algebraic version of the resonating group method. The calculated matrix element of the monopole transition from the ground state of 4 He into the O + -resonance state and the calculated monopole energy weighted sum rules confirm the assumption on the cluster nature of O + -resonance. In the dipole approximation the photonuclear reaction of 4 He(γ, n)He 3 at small energies of γ-quanta is studied. The obtained values of the electric dipole transition probabilities and the effective cross sections reveal a good agreement with experimental data

Gene Therapy for Human Lung Adenocarcinoma Using a Suicide Gene Driven by a Lung-Specific Promoter Delivered by JC Virus-Like Particles.

Directory of Open Access Journals (Sweden)

Chun-Nun Chao

Full Text Available Lung adenocarcinoma, the most commonly diagnosed type of lung cancer, has a poor prognosis even with combined surgery, chemotherapy, or molecular targeted therapies. Most patients are diagnosed with an in-operable advanced or metastatic disease, both pointing to the necessity of developing effective therapies for lung adenocarcinoma. Surfactant protein B (SP-B has been found to be overexpressed in lung adenocarcinoma. In addition, it has also been demonstrated that human lung adenocarcinoma cells are susceptible to the JC polyomavirus (JCPyV infection. Therefore, we designed that the JCPyV virus-like particle (VLP packaged with an SP-B promoter-driven thymidine kinase suicide gene (pSPB-tk for possible gene therapy of human lung adenocarcinoma. Plasmids expressing the GFP (pSPB-gfp or thymidine kinase gene (pSPB-tk under the control of the human SP-B promoter were constructed. The promoter's tissue specificity was tested by transfection of pSPB-gfp into A549, CH27, and H460 human lung carcinoma cells and non-lung cells. The JCPyV VLP's gene transfer efficiency and the selective cytotoxicity of pSPB-tk combined with ganciclovir (GCV were tested in vitro and in a xenograft mouse model. In the current study, we found that SP-B promoter-driven GFP was specifically expressed in human lung adenocarcinoma (A549 and large cell carcinoma (H460 cells. JCPyV VLPs were able to deliver a GFP reporter gene into A549 cells for expression. Selective cytotoxicity was observed in A549 but not non-lung cells that were transfected with pSPB-tk or infected with pSPB-tk-carrying JCPyV VLPs. In mice injected with pSPB-tk-carrying JCPyV VLPs through the tail vein and treated with ganciclovir (GCV, a potent 80% inhibition of growth of human lung adenocarcinoma nodules resulted. The JCPyV VLPs combined with the use of SP-B promoter demonstrates effectiveness as a potential gene therapy against human lung adenocarcinoma.

Gene Therapy for Human Lung Adenocarcinoma Using a Suicide Gene Driven by a Lung-Specific Promoter Delivered by JC Virus-Like Particles.

Science.gov (United States)

Chao, Chun-Nun; Lin, Mien-Chun; Fang, Chiung-Yao; Chen, Pei-Lain; Chang, Deching; Shen, Cheng-Huang; Wang, Meilin

2016-01-01

Lung adenocarcinoma, the most commonly diagnosed type of lung cancer, has a poor prognosis even with combined surgery, chemotherapy, or molecular targeted therapies. Most patients are diagnosed with an in-operable advanced or metastatic disease, both pointing to the necessity of developing effective therapies for lung adenocarcinoma. Surfactant protein B (SP-B) has been found to be overexpressed in lung adenocarcinoma. In addition, it has also been demonstrated that human lung adenocarcinoma cells are susceptible to the JC polyomavirus (JCPyV) infection. Therefore, we designed that the JCPyV virus-like particle (VLP) packaged with an SP-B promoter-driven thymidine kinase suicide gene (pSPB-tk) for possible gene therapy of human lung adenocarcinoma. Plasmids expressing the GFP (pSPB-gfp) or thymidine kinase gene (pSPB-tk) under the control of the human SP-B promoter were constructed. The promoter's tissue specificity was tested by transfection of pSPB-gfp into A549, CH27, and H460 human lung carcinoma cells and non-lung cells. The JCPyV VLP's gene transfer efficiency and the selective cytotoxicity of pSPB-tk combined with ganciclovir (GCV) were tested in vitro and in a xenograft mouse model. In the current study, we found that SP-B promoter-driven GFP was specifically expressed in human lung adenocarcinoma (A549) and large cell carcinoma (H460) cells. JCPyV VLPs were able to deliver a GFP reporter gene into A549 cells for expression. Selective cytotoxicity was observed in A549 but not non-lung cells that were transfected with pSPB-tk or infected with pSPB-tk-carrying JCPyV VLPs. In mice injected with pSPB-tk-carrying JCPyV VLPs through the tail vein and treated with ganciclovir (GCV), a potent 80% inhibition of growth of human lung adenocarcinoma nodules resulted. The JCPyV VLPs combined with the use of SP-B promoter demonstrates effectiveness as a potential gene therapy against human lung adenocarcinoma.

Follistatin is a novel biomarker for lung adenocarcinoma in humans.

Directory of Open Access Journals (Sweden)

Fangfang Chen

Full Text Available Follistatin (FST, a single chain glycoprotein, is originally isolated from follicular fluid of ovary. Previous studies have revealed that serum FST served as a biomarker for pregnancy and ovarian mucinous tumor. However, whether FST can serve as a biomarker for diagnosis in lung adenocarcinoma of humans remains unclear.The study population consisted of 80 patients with lung adenocarcinoma, 40 patients with ovarian adenocarcinoma and 80 healthy subjects. Serum FST levels in patients and healthy subjects were measured using ELISA. The results showed that the positive ratio of serum FST levels was 51.3% (41/80, which was comparable to the sensitivity of FST in 40 patients with ovarian adenocarcinoma (60%, 24/40 using the 95th confidence interval for the healthy subject group as the cut-off value. FST expressions in lung adenocarcinoma were examined by immunohistochemical staining, we found that lung adenocarcinoma could produce FST and there was positive correlation between the level of FST expression and the differential degree of lung adenocarcinoma. Furthermore, the results showed that primary cultured lung adenocarcinoma cells could secrete FST, while cells derived from non-tumor lung tissues almost did not produce FST. In addition, the results of CCK8 assay and flow cytometry showed that using anti-FST monoclonal antibody to neutralize endogenous FST significantly augmented activin A-induced lung adenocarcinoma cells apoptosis.These data indicate that lung adenocarcinoma cells can secret FST into serum, which may be beneficial to the survival of adenocarcinoma cells by neutralizing activin A action. Thus, FST can serve as a promising biomarker for diagnosis of lung adenocarcinoma and a useful biotherapy target for lung adenocarcinoma.

Mechanisms of decreased intestinal epithelial proliferation and increased apoptosis in murine acute lung injury.

Science.gov (United States)

Husain, Kareem D; Stromberg, Paul E; Woolsey, Cheryl A; Turnbull, Isaiah R; Dunne, W Michael; Javadi, Pardis; Buchman, Timothy G; Karl, Irene E; Hotchkiss, Richard S; Coopersmith, Craig M

2005-10-01

The aim of this study was to determine the effects of acute lung injury on the gut epithelium and examine mechanisms underlying changes in crypt proliferation and apoptosis. The relationship between severity and timing of lung injury to intestinal pathology was also examined. Randomized, controlled study. University research laboratory. Genetically inbred mice. Following induction of acute lung injury, gut epithelial proliferation and apoptosis were assessed in a) C3H/HeN wild-type and C3H/HeJ mice, which lack functional Toll-like receptor 4 (n = 17); b) C57Bl/6 mice that received monoclonal anti-tumor necrosis factor-alpha or control antibody (n = 22); and c) C57Bl/6 wild-type and transgenic mice that overexpress Bcl-2 in their gut epithelium (n = 21). Intestinal epithelial proliferation and death were also examined in animals with differing degrees of lung inflammation (n = 24) as well as in a time course analysis following a fixed injury (n = 18). Acute lung injury caused decreased proliferation and increased apoptosis in crypt epithelial cells in all animals studied. C3H/HeJ mice had higher levels of proliferation than C3H/HeN animals without additional changes in apoptosis. Anti-tumor necrosis factor-alpha antibody had no effect on gut epithelial proliferation or death. Overexpression of Bcl-2 did not change proliferation despite decreasing gut apoptosis. Proliferation and apoptosis were not correlated to severity of lung injury, as gut alterations were lost in mice with more severe acute lung injury. Changes in both gut epithelial proliferation and death were apparent within 12 hrs, but proliferation was decreased 36 hrs following acute lung injury while apoptosis returned to normal. Acute lung injury causes disparate effects on crypt proliferation and apoptosis, which occur, at least in part, through differing mechanisms involving Toll-like receptor 4 and Bcl-2. Severity of lung injury does not correlate with perturbations in proliferation or death in the

Beryllium-induced immune response in C3H mice

Energy Technology Data Exchange (ETDEWEB)

Benson, J.M.; Bice, D.E.; Nikula, K.J. [and others

1995-12-01

Studies conducted at ITRI over the past several years have investigated whether Beagle dogs, monkeys, and mice are suitable models for human chronic beryllium-induced lung disease (CBD). Recent studies have focused on the histopathological and immunopathological changes occurring in A/J and C3H/HeJ mice acutely exposed by inhalation to Be metal. Lung lesions in both strains of mice included focal lymphocyte aggregates comprised primarily of B lymphocytes and lesser amounts of T-helper lymphocytes and microgranulomas consisting chiefly of macrophages and T-helper lymphocytes. The distribution of proliferating cells within the microgranulomas was similar to the distribution of T-helper cells. These results strongly suggested that A/J and C3H/HeJ mice responded to inhaled Be metal in a fashion similar to humans in terms of pulmonary lesions and the apparent in situ proliferation of T-helper cells. Results of these studies confirm lymphocyte involvement in the pulmonary response to inhaled Be metal.

Non-uniform 3He polarization formed by multiple collisions of a fast 3He+ ion with polarized Rb vapor in a strong magnetic field

International Nuclear Information System (INIS)

Arimoto, Y.; Yonehara, K.; Yamagata, T.; Tanaka, M.

2001-01-01

We investigated the spatial distribution of a polarization in 3 He beam expected from a novel polarized 3 He ion source based on electron pumping, i.e., multiple electron capture and stripping collisions of an incident fast 3 He + ion with a polarized Rb vapor in a strong axial magnetic field. For this purpose, a Monte Carlo simulation was carried out for 19 keV 3 He + ions with varying Rb vapor thickness, magnetic field, and beam emittance. The calculated results showed a distribution of the 3 He polarization that we call a 'polarization hole', which has a low polarization area around the beam axis. The parameters characterizing the polarization hole, i.e., the polarization and radius of the hole, were found to depend on the Rb vapor thickness, the magnetic field, the beam size, and the angular divergence of the initial beam. These parameters were successfully reproduced with analytical functions deduced from a probability density function prescription. This provides a powerful tool to treat complex phenomena of multiple collisions in strong magnetic fields without performing time-consuming Monte Carlo calculations

D-3He fuel cycles for neutron lean reactors

International Nuclear Information System (INIS)

Kernbichler, W.; Miley, G.H.; Heindler, M.

1989-01-01

The intrinsic potential of D-3He as a reactor fuel is investigated for a large range of 3He to D density ratios. A steady-state zero-dimensional reactor model is developed in which much care is attributed to a proper treatment of fast fusion products. Useful ranges of reactor parameters as well as temperature-density windows for driven and ignited operation are identified. Various figures of merit are calculated, such as power densities, net power production, neutron production, tritium load and radiative power. These results suggest several optimistic conclusions about the performance of D-3He as a reactor fuel

Geometrical aspects of reaction cross sections for {sup 3}He, {sup 4}He and {sup 12}C projectiles

Energy Technology Data Exchange (ETDEWEB)

Ingemarsson, A. [Uppsala Univ. (Sweden). Dept. of Radiation Sciences; Lantz, M. [Uppsala Univ. (Sweden). The Svedberg Laboratory

2003-04-01

A black-disc model combined with accurate matter densities has been used for an investigation of reaction cross sections for {sup 3}He, {sup 4}He and {sup 12}C projectiles. A simple relation is derived between the energy dependence of the reaction cross sections and the strength of the nucleon-nucleon interaction. A comparison is also made of the reaction cross sections for {sup 3}He and {sup 4}He for six different nuclei {sup 12}C, {sup 16}O, {sup 40}Ca, {sup 58,60}Ni and {sup 208}Pb.

Polarized (3) He Spin Filters for Slow Neutron Physics.

Science.gov (United States)

Gentile, T R; Chen, W C; Jones, G L; Babcock, E; Walker, T G

2005-01-01

Polarized (3)He spin filters are needed for a variety of experiments with slow neutrons. Their demonstrated utility for highly accurate determination of neutron polarization are critical to the next generation of betadecay correlation coefficient measurements. In addition, they are broadband devices that can polarize large area and high divergence neutron beams with little gamma-ray background, and allow for an additional spin-flip for systematic tests. These attributes are relevant to all neutron sources, but are particularly well-matched to time of flight analysis at spallation sources. There are several issues in the practical use of (3)He spin filters for slow neutron physics. Besides the essential goal of maximizing the (3)He polarization, we also seek to decrease the constraints on cell lifetimes and magnetic field homogeneity. In addition, cells with highly uniform gas thickness are required to produce the spatially uniform neutron polarization needed for beta-decay correlation coefficient experiments. We are currently employing spin-exchange (SE) and metastability-exchange (ME) optical pumping to polarize (3)He, but will focus on SE. We will discuss the recent demonstration of 75 % (3)He polarization, temperature-dependent relaxation mechanism of unknown origin, cell development, spectrally narrowed lasers, and hybrid spin-exchange optical pumping.

Quenching of 4He(21S,21P) and 3He(21S,21P) states by collisions with Ne(1S0) atoms

International Nuclear Information System (INIS)

Blagoev, K.B.; Dimova, E.; Petrov, G.M.

2004-01-01

The cross sections and rate constants for quenching 4 He(2 1 S), 4 He(2 1 P), 3 He(2 1 S) and 3 He(2 1 P) states by collisions with ground state Ne atoms are measured by a time-resolved method in a He-Ne electron beam excited plasma at low pressure. These rate constants at T g =600 K are: k 4 He(2 1 S) =(1.6±0.2)x10 -10 , k 4 He(2 1 P) =(3.4±2.5)x10 -10 , k 3 He(2 1 S) =(1.6±0.2)x10 -10 and k 3 He(2 1 P) =(5.7±1.2)x10 -10 cm 3 s -1 . The cross sections derived from the rate constant are σ 4 He(2 1 S) =(8.4±0.8)x10 -16 , σ 4 He(2 1 P) =(1.8±1.3)x10 -15 , σ 3 He(2 1 S) =(7.1±0.9)x10 -16 and σ 3 He(2 1 P) =(2.6±0.5)x10 -15 cm 2 , respectively. The diffusion coefficient of 3 He(2 1 S) in 3 He is estimated to be D 3 He(2 1 S)- 3 He =1.9D 4 He(2 1 S)- 4 He , based on comparison with 4 He. A time-dependent collisional radiative model for an e-beam sustained He-Ne plasma is developed and the predicted line intensity of NeI λ=6328 A line is compared with the experimental data. The influence of different processes involved in population and depopulation dynamics of He(2 1 S) state are evaluated

γ-Tocotrienol Inhibits Proliferation and Induces Apoptosis via the Mitochondrial Pathway in Human Cervical Cancer HeLa Cells

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Weili Xu

2017-08-01

Full Text Available γ-Tocotrienol, a kind of isoprenoid phytochemical, has antitumor activity. However, there is limited evidence that it has an effect on cervical cancer. In this study, the capacity to inhibit proliferation and induce apoptosis in human cervical cancer HeLa cells and the mechanism underlying these effects were examined. The results indicated that a γ-tocotrienol concentration over 30 μM inhibited the growth of HeLa cells with a 50% inhibitory concentration (IC50 of 46.90 ± 3.50 μM at 24 h, and significantly down-regulated the expression of proliferative cell nuclear antigen (PCNA and Ki-67. DNA flow cytometric analysis indicated that γ-tocotrienol arrested the cell cycle at G0/G1 phase and reduced the S phase in HeLa cells. γ-tocotrienol induced apoptosis of HeLa cells in a time- and dose-dependent manner. γ-tocotrienol-induced apoptosis in HeLa cells was accompanied by down-regulation of Bcl-2, up-regulation of Bax, release of cytochrome from mitochondria, activation of caspase-9 and caspase-3, and subsequent poly (ADP-ribose polymerase (PARP cleavage. These results suggested that γ-tocotrienol could significantly inhibit cell proliferation through G0/G1 cell cycle arrest, and induce apoptosis via the mitochondrial apoptotic pathway in human cervical cancer HeLa cells. Thus, our findings revealed that γ-tocotrienol may be considered as a potential agent for cervical cancer therapy.

γ-Tocotrienol Inhibits Proliferation and Induces Apoptosis Via the Mitochondrial Pathway in Human Cervical Cancer HeLa Cells.

Science.gov (United States)

Xu, Weili; Mi, Yaqing; He, Pan; He, Shenghua; Niu, Lingling

2017-08-04

γ-Tocotrienol, a kind of isoprenoid phytochemical, has antitumor activity. However, there is limited evidence that it has an effect on cervical cancer. In this study, the capacity to inhibit proliferation and induce apoptosis in human cervical cancer HeLa cells and the mechanism underlying these effects were examined. The results indicated that a γ-tocotrienol concentration over 30 μM inhibited the growth of HeLa cells with a 50% inhibitory concentration (IC 50 ) of 46.90 ± 3.50 μM at 24 h, and significantly down-regulated the expression of proliferative cell nuclear antigen (PCNA) and Ki-67. DNA flow cytometric analysis indicated that γ-tocotrienol arrested the cell cycle at G0/G1 phase and reduced the S phase in HeLa cells. γ-tocotrienol induced apoptosis of HeLa cells in a time- and dose-dependent manner. γ-tocotrienol-induced apoptosis in HeLa cells was accompanied by down-regulation of Bcl-2, up-regulation of Bax, release of cytochrome from mitochondria, activation of caspase-9 and caspase-3, and subsequent poly (ADP-ribose) polymerase (PARP) cleavage. These results suggested that γ-tocotrienol could significantly inhibit cell proliferation through G0/G1 cell cycle arrest, and induce apoptosis via the mitochondrial apoptotic pathway in human cervical cancer HeLa cells. Thus, our findings revealed that γ-tocotrienol may be considered as a potential agent for cervical cancer therapy.

Conceptual design of D-3He FRC reactor 'ARTEMIS'

International Nuclear Information System (INIS)

Momota, H.; Ishida, A.; Kohzaki, Y.

1991-07-01

A comprehensive design study of the D- 3 He fueled field-reversed configuration (FRC) reactor 'ARTEMIS' is carried out for the purpose of proving its attractive characteristics and clarifying the critical issues for a commercial fusion reactor. The FRC burning plasma is stabilized and sustained in a steady equilibrium by means of a preferential trapping of D- 3 He fusion-produced energetic protons. A novel direct energy converter for 15MeV protons is also presented. On the bases of a consistent scenario of the fusion plasma production and simple engineering, a compact and simple reactor concept is presented. The design of the D- 3 He FRC power plant definitely offers the most attractive prospect for energy development. It is environmentally acceptable in view of radio-activity and fuel resources; and the estimated cost of electricity is low compared to a light water reactor. Critical issues concerning physics or engineering for the development of the D- 3 He FRC reactor are clarified. (author)

Elastic and charge-exchange scattering of pions from 3He and 3H

International Nuclear Information System (INIS)

Gibson, B.F.; Hess, A.T.

1976-04-01

We have examined (1) the elastic scattering of pions from the isodoublet 3 He and 3 H and (2) the single charge-exchange reaction 3 H(π + ,π 0 ) 3 He using a formalism which incorporates the π-N multiple scattering to all orders. Emphasis is placed on numerical results which illustrate those features of the differential cross sections that are expected to be of interest to the experimentalist. Realistic nuclear densities corresponding to the form factors of elastic electron scattering were used. Charge-exchange cross sections are presented in terms of angular distributions for both the π 0 and the recoil nucleus. In elastic scattering, Coulomb-nuclear interference effects are significant at incident pion kinetic energies of less than 100 MeV; form factor effects are apparent at large momentum transfer. Comparison of data and theory for π + - 3 He with that for π - - 3 He (or the conjugate π + - 3 H) will provide a test of the convergence of the fixed scatterer, multiple-scattering formalism utilized in this report. 21 figures

Polarization transfer in the 3H(rvec p,rvec n)3He reaction and the 0- level in 4He

International Nuclear Information System (INIS)

Walston, J.R.; Gould, C.R.; Haase, D.G.; Raichle, B.W.; Seely, M.L.; Walston, J.R.; Keith, C.D.; Gould, C.R.; Haase, D.G.; Raichle, B.W.; Seely, M.L.; Tornow, W.; Wilburn, W.S.; Keith, C.D.; Tornow, W.; Wilburn, W.S.; Hoffmann, G.W.; Penttilae, S.I.

1998-01-01

Longitudinal polarization-transfer coefficients for the 3 H(rvec p,rvec n) 3 He reaction have been measured at zero degrees for proton energies of 1.3 endash 2.8 MeV. The results show a striking resonance behavior for energies corresponding to excitation of the 0 - level in 4 He at 21.0 MeV. In agreement with R-matrix calculations, the value approaches unity at 1.52 MeV, the peak of the resonance. Near this same energy, at 1.62 MeV, the transverse polarization-transfer coefficient was measured to be consistent with zero. copyright 1998 The American Physical Society

The relation between temperature and concentration gradients in superfluid sup 3 He- sup 4 He solutions

CERN Document Server

Zadorozhko, A A; Rudavskij, E Y; Chagovets, V K; Sheshin, G A

2003-01-01

The temperature and concentration gradients nabla T and nabla x in a superfluid sup 3 He- sup 4 He mixture with an initial concentration 9,8 % of sup 3 He are measured in a temperature range 70-500 mK. The gradients are produced by a steady thermal flow with heating from below. It is shown that the value of nabla x/nabla T observed in the experiment is in good agreement with the theoretical model derived from the temperature and concentration dependences of osmotic pressure. The experimental data permitted us to obtain a thermal diffusion ratio of the solution responsible for the thermal diffusion coefficient.

Recent {sup 3}He radio frequency heating experiments on JET

Energy Technology Data Exchange (ETDEWEB)

Van Eester, D. [Association Euratom-Belgian State, LPP-ERM/KMS, TEC, Brussels (Belgium); Imbeaux, F.; Joffrin, E. [Association Euratom-CEA Cadarache, 13 - Saint-Paul-lez-Durance (France)] [and others

2003-07-01

Various ITER relevant experiments using {sup 3}He in a majority D plasma were performed in the recent JET campaigns. Two types can be distinguished: dedicated studies of the various RF heating scenarios which rely on the presence of {sup 3}He, and physics studies using RF heating as a working tool to provide a tunable heat source. As the success of a number of these experiments depended on the capability to keep the {sup 3}He concentration fixed, real time control of the {sup 3}He concentration was developed and used. This paper presents a brief overview of the results obtained, zooms in on some of the more interesting recent findings and discusses some of the theoretical background. (authors)

Murine respiratory mycoplasmosis (MRM) in C57BL/6N and C3H/HeN mice: strain differences in early host responses and exacerbation by nitrogen dioxide

International Nuclear Information System (INIS)

Parker, R.F.

1987-01-01

The studies reported here used genetic differences in susceptibility of C57BL/6N and C3H/HeN mice and exacerbation of the disease by nitrogen dioxide (NO 2 ) as tools in assessing the role of early host responses in the pathogenesis of MRM. The two strains did not differ in susceptibility to infection, but C3H/HeN mice were more susceptible to and had increased severity of lung lesions 14 days after intranasal inoculation as determined by 50% biological endpoints and morphometric analysis of tissues. Exposure to NO 2 for 4 hours prior to exposure to infectious aerosols exacerbated murine respiratory mycoplasmosis (MRM) by 7 days after exposure in both mouse strains. NO 2 appeared to affect host lung defense mechanisms responsible for limiting mycoplasmal growth in the lungs. The NO 2 exposure concentration required for this effect varied with the genetic background of the host, the dose of mycoplasmas administered, and the endpoint measured. Pulmonary clearance of radiolabeled M. pulmonis was determined in both mouse strains, and in C57BL/6N mice exposed to NO 2

One-body density matrix and the momentum density in 4He and 3He

International Nuclear Information System (INIS)

Whitlock, P.A.; Panoff, R.M.

1984-01-01

The one-body density matrix and the momentum density for liquid and solid 4 He, determined from Green's Function Monte Carlo calculations using the HFDHE2 pair potential, are described. Values for the condensate fraction and the kinetic energy derived from these calculations are given and compared to recent experimental results. Preliminary results from variational Monte Carlo calculations on n(r) and n(k) for liquid 3 He are also reported

Measurement of fast minority /sub 3/He/sup + +/ energy distribution during ICRF heating

Science.gov (United States)

Post, D.E. Jr.; Grisham, L.R.; Medley, S.S.

A method and means for measuring the fast /sub 3/He/sup + +/ distribution during /sub 3/He/sup + +/ minority Ion Cyclotron Resonance Frequency (ICRF) heating is disclosed. The present invention involves the use of 10 to 100 keV beams of neutral helium atoms to neutralize the fast /sub 3/He/sup + +/ ions in a heated plasma by double charge exchange (/sub 3/He/sup + +/ + /sub 4/He/sup 0/ ..-->.. /sub 3/He/sup 0/ + /sub 4/He/sup + +/). The neutralized fast /sub 3/He/sup 0/ atoms then escape from the hot plasma confined by a magnetic field and are detected by conventional neutral particle analyzing means. This technique permits the effectiveness of the coupling of the ion cyclotron waves to the /sub 3/He/sup + +/ minority ions to be accurately measured. The present invention is particularly adapted for use in evaluating the effectiveness of the intermediate coupling between the RF heating and the /sub 3/He/sup + +/ in an energetic toroidal plasma.

GSK-3α Is a Novel Target of CREB and CREB-GSK-3α Signaling Participates in Cell Viability in Lung Cancer.

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Sin-Aye Park

Full Text Available Overexpression or activation of cyclic AMP-response element-binding protein (CREB has been known to be involved in several human malignancies, including lung cancer. Genes regulated by CREB have been reported to suppress apoptosis, induce cell proliferation, inflammation, and tumor metastasis. However, the critical target genes of CREB in lung cancer have not been well understood. Here, we identified GSK-3α as one of the CREB target genes which is critical for the viability of lung cancer cells. The CREB knockdown significantly reduced the expression of GSK-3α and the direct binding of CREB on the promoter of GSK3A was identified. Kaplan-Meier analysis with a public database showed a prognostic significance of aberrant GSK-3α expression in lung cancer. Inhibition of GSK-3α suppressed cell viability, colony formation, and tumor growth. For the first time, we demonstrated that GSK-3α is regulated by CREB in lung cancer and is required for the cell viability. These findings implicate CREB-GSK-3α axis as a novel therapeutic target for lung cancer treatment.

Experimental measurements of 3He and 4He mobility in olivine and clinopyroxene at magmatic temperatures

Science.gov (United States)

Trull, T. W.; Kurz, M. D.

1993-03-01

In-vacuo heating of 0.5 -0.7 mm olivine and clinopyroxene grains separated from Hawaiian ultramafic xenoliths leads to complete He loss within hours to days for temperatures of 700-1400°C. Diffusivities calculated from the observed release rates assuming spherical grains and initially homogeneous He distributions define Arrhenius relations with activation energies of 420 ± 20 and 290 ± 40 Kj/mol and log 10D0 of +5.1 ± .7 and +2.1 ± 1.2 cm 2/s in olivine and pyroxene, respectively. Values at 1350°C are 5.3 × 10 -9 cm 2/s in olivine and 10 times faster in pyroxene (4.7 × 10 -8 cm 2/s). These values include small corrections for grain size variations and, in the case of olivine, about 15% prior diffusive He loss. However, an important factor that has not been considered in previous studies of this type, is that the xenolith He resides predominantly within CO 2 rich fluid inclusions. Theoretical description of He loss in such a case demonstrates that the diffusivities calculated using the standard approach actually represent the product of the true volume diffusivity ( D) and the helium solubility, as represented by the distribution coefficient KDv (defined by C crystal/C fluid). Although He solubility in crystals is not well determined, estimates based on the CO 2 concentrations in these samples suggest that it is very low ( KDv of 3 × 10 -4 for pyroxene and 6 × 10 -6 for olivine; which also implies low crystal-melt distribution coefficients of .05 and .001). The resultant corrected diffusion rates are significantly faster than those obtained by the standard approach (~ 10 -4 cm 2/s at 1350°C and are thus higher than basaltic melt values). The most reasonable interpretation of this result is that He release is enhanced by internal grain fractures, including the planar healed cracks along which most fluid inclusions are arrayed. This treatment illustrates the difficulties involved in extrapolating laboratory He release measurements to nature, in particular

Surface study of liquid 3He using surface state electrons

International Nuclear Information System (INIS)

Shirahama, K.; Ito, S.; Suto, H.; Kono, K.

1995-01-01

We have measured the mobility of surface state electrons (SSE) on liquid 3 He, μ 3 , aiming to study the elementary surface excitations of the Fermi liquid. A gradual increase of μ 3 below 300 mK is attributed to the scattering of electrons by ripplons. Ripplons do exist in 3 He down to 100 mK. We observe an abrupt decrease of μ 3 , due to the transition to the Wigner solid (WS). The dependences of the WS conductivity and mobility on temperature and magnetic field differ from the SSE behavior on liquid 4 He

Apparent CFC and 3H/ 3He age differences in water from Floridan Aquifer springs

Science.gov (United States)

Happell, James D.; Opsahl, Stephen; Top, Zafer; Chanton, Jeffrey P.

2006-03-01

The apparent CFC-11, -12 and -113 ages of Upper Floridan Aquifer water discharged from 31 springs located in Florida and Georgia ranged from 11 to 44 years when samples were collected in 2002 and 2003. Apparent 3H/ 3He ages in these springs ranged from 12 to 66 years. Some of the springs sampled did not yield valid CFC ages because one or more of the CFCs were contaminated by non-atmospheric sources. Of the 31 springs sampled, six were contaminated with all three CFCs and nine were contaminated with one or two CFCs. Of the remaining 16 springs, the CFC distributions of four could be modeled assuming a single source of water, and 11 were best modeled by assuming two sources of water, with one of the water sources >60 years old. The CFC and 3H/ 3He apparent ages and the simple mixing models applied to these ages suggest that past impacts to the water quality of water recharging the sampled springs may take anywhere from 0 to ˜60 years or more to appear in the discharging spring water. In 27 springs where both 3H/ 3He ages and CFC ages were available, five springs gave similar results between the two techniques, while in the other 22 cases the 3H/ 3He apparent ages were 8-40 years greater than the CFC ages. Large excesses of 4He were observed in many of the springs, consistent with a source of older water. This older water may also carry an additional and unaccounted for source of 3He, which may be responsible for the greater 3H/ 3He ages relative to the CFC ages. We believe that the large excess 3He and 4He values and apparent age differences are related to regional climate variations because our samples were obtained at the end of a 4-year drought.

Human Organotypic Lung Tumor Models: Suitable For Preclinical 18F-FDG PET-Imaging.

Directory of Open Access Journals (Sweden)

David Fecher

Full Text Available Development of predictable in vitro tumor models is a challenging task due to the enormous complexity of tumors in vivo. The closer the resemblance of these models to human tumor characteristics, the more suitable they are for drug-development and -testing. In the present study, we generated a complex 3D lung tumor test system based on acellular rat lungs. A decellularization protocol was established preserving the architecture, important ECM components and the basement membrane of the lung. Human lung tumor cells cultured on the scaffold formed cluster and exhibited an up-regulation of the carcinoma-associated marker mucin1 as well as a reduced proliferation rate compared to respective 2D culture. Additionally, employing functional imaging with 2-deoxy-2-[18F]fluoro-D-glucose positron emission tomography (FDG-PET these tumor cell cluster could be detected and tracked over time. This approach allowed monitoring of a targeted tyrosine kinase inhibitor treatment in the in vitro lung tumor model non-destructively. Surprisingly, FDG-PET assessment of single tumor cell cluster on the same scaffold exhibited differences in their response to therapy, indicating heterogeneity in the lung tumor model. In conclusion, our complex lung tumor test system features important characteristics of tumors and its microenvironment and allows monitoring of tumor growth and -metabolism in combination with functional imaging. In longitudinal studies, new therapeutic approaches and their long-term effects can be evaluated to adapt treatment regimes in future.

Spin-polarized electron capture for the Na+3He2+ system at a 3He2+ impact energy of 5.33--9.33 keV/amu

International Nuclear Information System (INIS)

Tanaka, M.; Shimakura, N.; Ohshima, T.; Katori, K.; Fujiwara, M.; Ogata, H.; Kondo, M.

1994-01-01

3 He + atomic polarizations following the spin-polarized electron capture process for the N rvec a(3s)+ 3 He 2+ system were measured at 3 He 2+ impact energies from 5.33 to 9.33 keV/amu. The magnitude of the 3 He + atomic polarizations was deduced from the 3 He + nuclear polarization measured by means of beam-foil spectroscopy. The observed polarization transfer coefficient P T defined by the ratio of the 3 He + atomic polarization to the sodium one showed a pronounced reduction from unity, which was qualitatively explained by the prediction of a simple cascade photon decay model. Evidence for a further reduction of P T from the above model and a possible impact energy dependence of P T suggested an excessive depolarization due to the presence of the collision alignment parameter A 0 col of 3 He + formed by the electron capture process. In order to see this more closely, the observed P T 's were examined theoretically using the semiclassical impact parameter method, in which an 18-state molecular expansion was employed, and atomic-type electron translation effects were rigorously taken into account. Ensuring that both the absolute values and the impact energy dependence of the observed capture cross sections were remarkably well reproduced by the calculations in which the states up to 4f in 3 He + were introduced, it was demonstrated that the calculated results for P T qualitatively reproduced not only the absolute values of the observed P T 's but also their gentle decrease with increasing impact energy. Production of nuclear polarizations resulting from the polarized electron capture processes between multicharged heavy ions and alkaline-earth-metal atoms is an example of one use of the future project of universal polarized heavy-ion sources

Observations on the expression of human papillomavirus major capsid protein in HeLa cells.

Science.gov (United States)

Xiao, Chang-Yi; Fu, Bing-Bing; Li, Zhi-Ying; Mushtaq, Gohar; Kamal, Mohammad Amjad; Li, Jia-Hua; Tang, Gui-Cheng; Xiao, Shuo-Shuang

2015-01-01

The goal of this study was to identify the nature of the inclusion bodies that have been found in HeLa cells (cervical cancer immortal cell line) by electron microscope and to determine whether the major capsid protein (L1) of human papillomavirus (HPV) can be expressed in HPV-positive uterine cervix cancer cells. HPV L1 protein expression in HeLa cells was detected with anti-HPV L1 multivalent mice monoclonal antibody and rabbit polyclonal anti-HPV L1 antibody by ELISA, light microscope immunohistochemistry, electron microscope immunocytochemistry and Western blotting assays. Reverse transcriptional PCR (RT-PCR) was performed to detect the transcription of L1 mRNA in HeLa cells. The immortalized human keratinocyte HeCat was used as the negative control. HPV L1 proteins reacted positively in the lysate of HeLa cells by ELISA assays. HRP labeled light microscope immunohistochemistry assay showed that there was a strong HPV L1 positive reaction in HeLa cells. Under the electron microscope, irregular shaped inclusion bodies, assembled by many small and uniform granules, had been observed in the cytoplasm of some HeLa cells. These granules could be labeled by the colloidal gold carried by HPV L1 antibody. The Western blotting assay showed that there was a L1 reaction strap at 80-85 kDa in the HeLa cell lysates, hence demonstrating the existence of HPV18 L1 in HeLa cells. RT-PCR assay showed that the L1 mRNA was transcribed in HeLa cells. The inclusion bodies found in the cytoplasm of HeLa cells are composed of HPV18 L1 protein. Since HeLa cell line is a type of cervical cancer cells, this implies that HeLa cells have the ability to express HPV L1 proteins.

Terrestrial cosmogenic 3He: where are we 30 years after its discovery?

Science.gov (United States)

Blard, Pierre-Henri; Pik, Raphaël; Farley, Kenneth A.; Lavé, Jérôme; Marrocchi, Yves

2016-04-01

It is now 30 years since cosmogenic 3He has been detected for the first time in a terrestrial sample (Kurz, 1986). 3He is now a widely used geochemical tool in many fields of Earth sciences: volcanology, tectonics, paleoclimatology. 3He has the advantage to have a high "production rate" to "detection limit" ratio, allowing surfaces as young as hundred of years to be dated. Although its nuclear stability implies several limitations, it moreover represents a useful alternative to 10Be in mafic environments. This contribution is a review of the progresses that have been accomplished since this discovery, and discuss strategies to improve both the accuracy and the precision of this geochronometer. 1) Measurement of cosmogenic 3He Correction of magmatic 3He. To estimate the non-cosmogenic magmatic 3He, Kurz (1986) invented a two steps method involving crushing of phenocrysts (to analyze the isotopic ratio of the magmatic component), followed by a subsequent melting of the sample, to extract the remaining components, including the cosmogenic 3He: 3Hec = 3Hemelt -4Hemelt x (3He/4He)magmatic (1) Several studies suggested that the preliminary crushing may induce a loss of cosmogenic 3He (Hilton et al., 1993; Yokochi et al., 2005; Blard et al., 2006), implying an underestimate of the cosmogenic 3He measurement. However, subsequent work did not replicate these observations (Blard et al., 2008; Goerhing et al., 2010), suggesting an influence of the used apparatus. An isochron method (by directly melting several phenocrysts aliquots) is an alternative to avoid the preliminary crushing step (Blard and Pik, 2008). Atmospheric contamination. Protin et al. (in press) provides robust evidences for a large and irreversible contamination of atmospheric helium on silicate surfaces. This unexpected behavior may reconcile the contrasted observations about the amplitude of crushing loss. This undesirable atmospheric contamination is negligible if grain fractions smaller than 150 mm are

Estimation of lung volume and pulmonary blood volume from radioisotopic images

International Nuclear Information System (INIS)

Kanazawa, Minoru

1989-01-01

Lung volume and pulmonary blood volume in man were estimated from the radioisotopic image using single photon emission computed tomography (SPECT). Six healthy volunteers were studied in a supine position with normal and altered lung volumes by applying continuous negative body-surface pressure (CNP) and by positive end-expiratory pressure (PEEP). 99m Tc labeled human serum albumin was administered as an aerosol to image the lungs. The CNP caused the diaphragm to be lowered and it increased the mean lung tissue volume obtained by SPECT from 3.09±0.49 l for baseline to 3.67±0.62 l for 10 cmH 2 O (p 2 O (p 2 O), respectively. The PEEP also increased the lung tissue volume to 3.68±0.68 l for 10 cmH 2 O as compared with the baseline (p 2 O PEEP. The lung tissue volume obtained by SPECT showed a positive correlation with functional residual capacity measured by the He dilution method (r=0.91, p 99m Tc-labeled red blood cells. The L/H ratio decreased after either the CNP or PEEP, suggesting a decrease in the blood volume per unit lung volume. However, it was suggested that the total pulmonary blood volume increased slightly either on the CNP (+7.4% for 10 cmH 2 O, p 2 O,p<0.05) when we extrapolated the L/H ratio to the whole lungs by multiplying the lung tissue volume obtained by SPECT. We concluded that SPECT could offer access to the estimation of lung volume and pulmonary blood volume in vivo. (author)

Diffusion on Networks and Diffusion Weighted NMR of the Human Lung

DEFF Research Database (Denmark)

Buhl, Niels

2011-01-01

of the diffusion propagator to general properties of the underlying graph. Diffusion weighted NMR of the human lung with hyperpolarized noble gases, which over the last decade has been demonstrated to be a very promising way of detecting and quantifying lung diseases like emphysema, represent an obvious...... application of the above mentioned theory, given that the human lung consists of a large network of bifurcating tube like airways. 90-95% of the gas in a human lung resides in the ~30000 pulmonary acini, each of these consists of ~500 airways, which are connected as the edges in a binary tree. We model...... diffusion in the pulmonary acini as diffusion on metric graphs with this structure. The metric graph for each individual pulmonary acinus is embedded in three dimensional space via line segments. By considering an isotropic distribution of acini and a symmetric branching geometry for the line segments...

Fabrication and characterization of scaffold from cadaver goat-lung tissue for skin tissue engineering applications.

Science.gov (United States)

Gupta, Sweta K; Dinda, Amit K; Potdar, Pravin D; Mishra, Narayan C

2013-10-01

The present study aims to fabricate scaffold from cadaver goat-lung tissue and evaluate it for skin tissue engineering applications. Decellularized goat-lung scaffold was fabricated by removing cells from cadaver goat-lung tissue enzymatically, to have cell-free 3D-architecture of natural extracellular matrix. DNA quantification assay and Hematoxylin and eosin staining confirmed the absence of cellular material in the decellularized lung-tissue. SEM analysis of decellularized scaffold shows the intrinsic porous structure of lung tissue with well-preserved pore-to-pore interconnectivity. FTIR analysis confirmed non-denaturation and well maintainance of collagenous protein structure of decellularized scaffold. MTT assay, SEM analysis and H&E staining of human skin-derived Mesenchymal Stem cell, seeded over the decellularized scaffold, confirms stem cell attachment, viability, biocompatibility and proliferation over the decellularized scaffold. Expression of Keratin18 gene, along with CD105, CD73 and CD44, by human skin-derived Mesenchymal Stem cells over decellularized scaffold signifies that the cells are viable, proliferating and migrating, and have maintained their critical cellular functions in the presence of scaffold. Thus, overall study proves the applicability of the goat-lung tissue derived decellularized scaffold for skin tissue engineering applications. Copyright © 2013 Elsevier B.V. All rights reserved.

The πHe3H3 coupling constant estimation using the Chew-Low equation

International Nuclear Information System (INIS)

Mach, R.; Nichitiu, F.

1976-01-01

A semi-phenomenological analysis of the π +- He 3 elastic scattering at 98, 120, 135 and 156 Mev is presented. An information of the πHe 3 H 3 coupling constant using the Chew-Low plot for the P 33 partial wave is obtained. (author)

The πHe3H3 coupling constant estimation using the Chew-Low equation

International Nuclear Information System (INIS)

Mach, R.; Nichitiu, F.

1975-01-01

In this paper it is presented an estimation of the πHe 3 H 3 coupling constant using the Chew-Low equation and a semi-phenomenological analysis of the π -+ He 3 elastic differential cross sections at 98, 120, 135 and 156 MeV

Development of a 3He nuclear spin flip system on an in-situ SEOP 3He spin filter and demonstration for a neutron reflectometer and magnetic imaging technique

International Nuclear Information System (INIS)

Hayashida, H; Kira, H; Miyata, N; Akutsu, K; Mizusawa, M; Parker, J D; Matsumoto, Y; Oku, T; Sakai, K; Hiroi, K; Shinohara, T; Takeda, M; Yamazaki, D; Oikawa, K; Harada, M; Ino, T; Imagawa, T; Ohkawara, M; Ohoyama, K; Kakurai, K

2016-01-01

We have been developing a 3 He neutron spin filter (NSF) using the spin exchange optical pumping (SEOP) technique. The 3 He NSF provides a high-energy polarized neutron beam with large beam size. Moreover the 3 He NSF can work as a π-flipper for a polarized neutron beam by flipping the 3 He nuclear spin using a nuclear magnetic resonance (NMR) technique. For NMR with the in-situ SEOP technique, the polarization of the laser must be reversed simultaneously because a non-reversed laser reduces the polarization of the spin-flipped 3 He. To change the polarity of the laser, a half-wavelength plate was installed. The rotation angle of the half-wavelength plate was optimized, and a polarization of 97% was obtained for the circularly polarized laser. The 3 He polarization reached 70% and was stable over one week. A demonstration of the 3 He nuclear spin flip system was performed at the polarized neutron reflectometer SHARAKU (BL17) and NOBORU (BL10) at J-PARC. Off-specular measurement from a magnetic Fe/Cr thin film and magnetic imaging of a magnetic steel sheet were performed at BL17 and BL10, respectively. (paper)

Quasi-free and sequential processes in the 9Be(3He,αα)4He reaction at 2,8 MeV

International Nuclear Information System (INIS)

Barbarino, S.; Lattuada, M.; Riggi, F.; Spitaleri, C.; Vinciguerra, D.

1979-01-01

The quasi-free contribution to the 9 Be( 3 He,αα) 4 He reaction at low incident energy is studied under various detection configurations. The measurements performed at quasi-free angles (symmetrical and non-symmetrical configurations) furter confirm that a large region in the energy spectra is dominated by the quasi-free 5 He( 3 He,α) 4 He process. From a comparison of these results with those taken at symmetrical angles where the minimum accepted momentum of the spectator is around 70 MeV/c, the conclusion is drawn that no significant distortion is introduced by the sequential peak tails in the deduced 5 He- 4 He momentum distribution

Spin-polarized 3He nuclear targets and metastable 4He atoms by optical pumping with a tunable, Nd:YAP laser

International Nuclear Information System (INIS)

Bohler, C.L.; Schearer, L.D.; Leduc, M.; Nacher, P.J.; Zachorowski, L.; Milner, R.G.; McKeown, R.D.; Woodward, C.E.

1988-01-01

Several Nd:YAP lasers were constructed which could be broadly tuned in the 1083-nm region which includes the helium 2 3 S-2 3 P transition, using a Lyot filter and thin, uncoated etalons within the laser cavity. 1 W of power could be extracted at 1083 nm through a 1% transmitting output coupler. This laser beam was used to optically pump metastable 4 He and 3 He 2 3 S helium atoms in a weak discharge cell, spin polarizing the metastable ensemble. In a 3 He cell the polarization is transferred to the nuclear spin system. A 3 He target cell at 0.3 Torr was polarized to 52% in a few minutes. We describe the application of this system to the design of polarized targets for experiments in nuclear physics

Spin structure of the 3He from the dd → 3Hen reaction

International Nuclear Information System (INIS)

Ladygin, V.P.; Ladygina, N.B.

1995-01-01

The polarization observables in the reaction dd → 3 Hen are considered. Their high sensitivity to the 3 He wave function at short distances is shown. Using of both polarized target and beam allows to extend sufficiently the number of possible experiments and to separate 3 He structure from the reaction mechanisms using different relative orientations of initial deuteron spins. 27 refs., 5 figs

Biodistribution of 99mTc-labeled anti-human epidermal growth factor receptor (EGF-R) humanized monoclonal antibody h-R3 in a xenograft model of human lung adenocarcinoma

International Nuclear Information System (INIS)

Morales-Morales, Alejo; Duconge, Jorge; Caballero-Torres, Idania; Nunez-Gandolff, Gilda; Fernandez, Eduardo; Iznaga-Escobar, Normando

1999-01-01

The anti-human epidermal growth factor receptor (EGF-R) humanized monoclonal antibody (MAb) h-R3 is an (IgG 1 ), which binds to an extracellular domain of EGF-R. It was used to evaluate the biodistribution on nude mice xenografted with H-125 human lung adenocarcinoma cell line. Results were compared with its murine version of the MAb ior-egf/r3. Twenty-one athymic female 4NMRI nu/nu mice were injected intraperitoneally with 10 μg/100 μCi of 99m Tc-labeled MAbs. Immunoreactivity of 99m Tc-labeled MAbs were measured by enzyme-linked immunosorbent assay (ELISA) on H-125 cell line and the immunoreactive fractions was determined by the Lindmo method. Among all organs, significant accumulation was found in serum (27.05 ± 2.08 %ID/g) and tumor (3.903 ± 0.89 %ID/g) at 4 h after injection. These values decreased to 5.03 ± 0.50 %ID/g and 2.19 ± 0.56 %ID/g for serum and tumor, respectively. The immunoreactive fraction was found to be 0.70, with a correlation coefficient r=0.9984. With the good biodistribution and tumor uptake of the 99m Tc-labeled humanized antibody h-R3, a phase I diagnostic clinical trial of tumor with epithelial origin should be pursued

CNTN-1 Enhances Chemoresistance in Human Lung Adenocarcinoma Through Induction of Epithelial-Mesenchymal Transition by Targeting the PI3K/Akt Pathway

Directory of Open Access Journals (Sweden)

Ruijie Zhang

2017-09-01

Full Text Available Background/Aims: Chemoresistance has been a major obstacle to the effective treatment of lung cancer. Previously, we found that contactin-1 (CNTN-1 is related to cisplatin resistance in lung adenocarcinoma. Here, we aimed to investigate the underlying mechanism behind the role of CNTN-1 in cisplatin resistance in lung adenocarcinoma. Methods: EMT-associated phenotypes, including alterations in cellular morphology and marker (E-cadherin, N-cadherin and Vimentin expression, were compared between A549 cells and A549/DDP cells (a cisplatin-resistant cell line of lung adenocarcinoma with abnormal CNTN-1 expression by using real-time time PCR and Western blotting. Other methods, including CNTN-1 overexpression in A549 cells and CNTN-1 knockdown in A549/DDP cells, were also used to investigate the role of CNTN-1 in mediating the EMT phenotype and thr resulting cisplatin resistance and malignant progression of cancer cells in vitro and in vivo. Results: A549/DDP cells exhibited an EMT phenotype and aggravated malignant behaviors. CNTN-1 knockdown in A549/DDP cells partly reversed the EMT phenotype, increased drug sensitivity, and attenuated the malignant progression whereas CNTN-1 overexpression in A549 cells resulted in the opposite trend. Furthermore, the PI3K/Akt pathway was involved in the effects of CNTN-1 on EMT progression in A549/DDP cells, verified by the xenograft mouse model. Conclusion: CNTN-1 promotes cisplatin resistance in human cisplatin-resistant lung adenocarcinoma through inducing the EMT process by activating the PI3K/Akt signaling pathway. CNTN-1 may be a potential therapeutic target to reverse chemoresistance in cisplatin-resistant lung adenocarcinoma.

3-(3-Hydroxy-4-methoxyphenyl)-4-(3,4,5-trimethoxyphenyl)-1,2,5-selenadiazole (G-1103), a novel combretastatin A-4 analog, induces G2/M arrest and apoptosis by disrupting tubulin polymerization in human cervical HeLa cells and fibrosarcoma HT-1080 cells.

Science.gov (United States)

Zuo, Daiying; Guo, Dandan; Jiang, Xuewei; Guan, Qi; Qi, Huan; Xu, Jingwen; Li, Zengqiang; Yang, Fushan; Zhang, Weige; Wu, Yingliang

2015-02-05

Microtubule is a popular target for anticancer drugs. In this study, we describe the effect 3-(3-hydroxy-4-methoxyphenyl)-4-(3,4,5-trimethoxyphenyl)-1,2,5-selenadiazole (G-1103), a newly synthesized analog of combretastatin A-4 (CA-4), showing a strong time- and dose-dependent anti-proliferative effect on human cervical cancer HeLa cells and human fibrosarcoma HT-1080 cells. We demonstrated that the growth inhibitory effects of G-1103 in HeLa and HT-1080 cells were associated with microtubule depolymerization and proved that G-1103 acted as microtubule destabilizing agent. Furthermore, cell cycle analysis revealed that G-1103 treatment resulted in cell cycle arrest at the G2/M phase in a time-dependent manner with subsequent apoptosis induction. Western blot analysis revealed that down-regulation of cdc25c and up-regulation of cyclin B1 was related with G2/M arrest in HeLa and HT-1080 cells treatment with G-1103. In addition, G-1103 induced HeLa cell apoptosis by up-regulating cleaved caspase-3, Fas, cleaved caspase-8 expression, which indicated that G-1103 induced HeLa cell apoptosis was mainly associated with death receptor pathway. However, G-1103 induced HT-1080 cell apoptosis by up-regulating cleaved caspase-3, Fas, cleaved caspase-8, Bax and cleaved caspase-9 expression and down-regulating anti-apoptotic protein Bcl-2 expression, which indicated that G-1103 induced HT-1080 cell apoptosis was associated with both mitochondrial and death receptor pathway. Taken together, all the data demonstrated that G-1103 exhibited its antitumor activity through disrupting the microtubule assembly, causing cell cycle arrest and consequently inducing apoptosis in HeLa and HT-1080 cells. Therefore, the novel compound G-1103 is a promising microtubule inhibitor that has great potentials for therapeutic treatment of various malignancies. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Small objects in superfluid 3He

International Nuclear Information System (INIS)

Rainer, D.; Vuorio, M.

1977-02-01

Distortions in the superfluid order parameter around a small object in 3 He are calculated together with the supercurrents and the angular momentum induced by it in the liquid. The forces acting on the impurity by the liquid texture structure are also considered. (author)

A polarized solid {sup 3}He target for neutron transmission experiments

Energy Technology Data Exchange (ETDEWEB)

Keith, C.D. [North Carolina State Univ., Raleigh, NC (United States)]|[Triangle Universities Nuclear Laboratory, Durham, NC 27708-0308 (United States); Gould, C.R. [North Carolina State Univ., Raleigh, NC (United States)]|[Triangle Universities Nuclear Laboratory, Durham, NC 27708-0308 (United States); Haase, D.G. [North Carolina State Univ., Raleigh, NC (United States)]|[Triangle Universities Nuclear Laboratory, Durham, NC 27708-0308 (United States); Huffman, P.R. [Duke University, Durham, NC 27708-0308 (United States)]|[Triangle Universities Nuclear Laboratory, Durham, NC 27708-0308 (United States); Roberson, N.R. [Duke University, Durham, NC 27708-0308 (United States)]|[Triangle Universities Nuclear Laboratory, Durham, NC 27708-0308 (United States); Seely, M.L. [North Carolina State Univ., Raleigh, NC (United States)]|[Triangle Universities Nuclear Laboratory, Durham, NC 27708-0308 (United States); Tornow, W. [Duke University, Durham, NC 27708-0308 (United States)]|[Triangle Universities Nuclear Laboratory, Durham, NC 27708-0308 (United States); Wilburn, W.S. [Duke University, Durham, NC 27708-0308 (United States)]|[Triangle Universities Nuclear Laboratory, Durham, NC 27708-0308 (United States)

1995-04-01

We describe the construction and operation of a solid {sup 3}He polarized nuclear target which we have used for measurements of the spin dependence of the n-{sup 3}He interaction at MeV energies. The target, which contains 0.4 mole of {sup 3}He was polarized to 38% at 12 mK in a field of 7 T. The target is suitable for nuclear physics measurements which are insensitive to the large magnetic field and produce beam heating of tenths of microwatts.We discuss refinements and paths to improved solid {sup 3}He targets at higher polarizations and lower fields. ((orig.)).

Anti-EGFR therapy radiosensitizes human lung adenocarcinoma xenograft in nude mouse

International Nuclear Information System (INIS)

Wang Hui; Li Tianran; Tian Jiahe; Qu Baolin; Zhu Hui

2008-01-01

Objective: To investigate the effect of Gefitinib on radiosensitivity of human lung adenocarcinoma xenograft in nude mouse. Methods: Human lung adenocarcinoma cell line A549 was used to establish nude mouse xenograft tumor model. The mice were derided into 4 groups: control, irradiation alone, Gefinitib alone and radiation combined with Genifitib. Radiation schedule was 3 fractions of 5 Gy, once daily. Gefitinib was daily administered by gavage at 100 mg/(kg·day -1 ) for 14 days. In the combination group, radiotherapy was performed 2 hours after Gefitinib administration. Tumor diameter was measured every other day. Percentage of tumor growth inhibition, growth delay time and regrowth delay time were evaluated. Results: For A549 xenografts in radiation alone, gefitinib alone and combination therapy groups, the percentage of tumor growth inhibition was 22.7%, 12.4% and 38.2%, respectively (F=25.75, P=0.000). Tumor growth delay time was 6.0, 7.8 and 21.6 days, respectively (F=70.49, P=0.000). Tumor regrowth delay time in combination therapy and irradiation alone groups was 23.4 and 10.2 days. (F=174.24, P= 0.000). Sensitizing enhancement ratio of combination group was 1.5 in growth and 1.7 in regrowth. Conclusions: Anti-EGFR therapy enhances the radiosensitivity of human lung adenocarcinoma xenograft in nude mouse. (authors)

HE3 outgassing from four working palladium and uranium beds

International Nuclear Information System (INIS)

Souers, P. C.; Coronado, P. R.; Fearon, F. M.; Garza, R. G.; Shaw , J. F.; Stump, R. K.; Tsugawa, R. T.

1988-01-01

The He 3 output from two palladium and two uranium beds storing T 2 and D-T was studied as a function of time. Three of the beds were started new and watched for a year; the fourth bed was twelve years old. All four were beds used in routine tritium handling. Initial stoichiometries were PdT/sub 0.3/ and UT/sub 0.7/ so that both operated at similar 1 to 130 kPa pressures. The He 3 from palladium ranged from the 0.002 mo1% lower level of sensitivity to 0.01% for PdT 2 at one year of age. The UT system showed 0.1% He 3 at 4 to 62 days and 0.1 to 10% at longer times, with the first cuts being high in He 3 . The palladium bed with 95 to 97% pure T 2 enriches the output to as high as 97 to 99%. 9 refs., 1 fig., 2 tabs

Ab initio calculations of 3H(d,n)4He fusion

International Nuclear Information System (INIS)

Navratil, Petr; Quaglioni, Sofia

2012-01-01

We build a new ab initio many-body approach capable of describing simultaneously both bound and scattering states in light nuclei, by combining the resonating-group method with the ab initio no-core shell model. In this way, we complement a microscopic-cluster technique with the use of realistic interactions, and a microscopic and consistent description of the nucleon clusters. We will present the first results of the d- 3 H and d- 3 He fusion calculation obtained within our ab initio approach. We will also discuss our d- 4 He, 3 H- 4 He and 3 H- 3 H scattering calculations and the outline of the extension of the formalism to include three-cluster final states with the goal to calculate the 3 H( 3 H,2n) 4 He cross section

Progress in Polarized 3He Ion Source at RCNP

International Nuclear Information System (INIS)

Tanaka, M.; Takahashi, Y.; Shimoda, T.; Yasui, S.; Yosoi, M.; Takahisa, K.; Shimakura, N.; Plis, Yu. A.; Donets, E. D.

2007-01-01

A long history on the polarized 3He ion source developed at RCNP is presented. We started with an 'OPPIS' (Optical Pumping Polarized Ion Source) and later found the fundamental difficulties in the OPPIS. To overcome them an 'EPPIS' (Electron Pumping Polarized Ion Source) was proposed and its validity was experimentally proven. However, a serious technical disadvantage was also found in the EPPIS. To avoid this disadvantage we proposed a new concept, 'SEPIS' (Spin Exchange Polarized Ion Source), which uses an enhanced spin-exchange cross section theoretically expected at low 3He+ incident energies in the 3He+ + Rb system. Next, we describe the present status of the SEPIS development: construction of a bench test device allowing the measurements of not only the spin-exchange cross sections σse but also the electron capture cross sections σec for the 3He+ + Rb system. The latest experimental data on σec are presented and compared with other previous experimental data and the theoretical calculations.Finally, a design study of the SEPIS for practical use in nuclear (cyclotron) and particle physics (synchrotron) is shortly mentioned

INVASIVE SALMONELLOSIS PRESENTING AS A LUNG ABSCESS: A CASE REPORT.

Science.gov (United States)

Songkhla, Munjit Na; Chayakulkeeree, Methee

2017-01-01

Salmonella spp are an uncommon cause of lung abscess. A 59 year old man presented to our hospital with a 1 month history of cough and low grade fever progressing to high grade fever for 1 week. He had a past medical history significant for diabetes mellitus type 2 and focal segmental glomerulosclerosis for which he was receiving prednisolone, initially at 60 mg daily tapering to 20 mg daily. On presentation he was febrile and had decreased breath sounds and dullness to percussion over the right lower lung field. A chest X-ray showed a cavitary lesion with an air-fluid level in the right lung. Computed tomography of the lung revealed 2 cavitary lesions in the right upper and lower lungs. Sputum culture revealed Salmonella spp group B. He was treated successfully with ceftriaxone intravenously for 1 month followed by oral cefdinir. A chest X-ray at 1 month showed significant improvement; he was treated conservatively without surgical drainage. Salmonella can cause lung abscesses, especially in the immune suppressed.

Recombinant human endostatin improves tumor vasculature and alleviates hypoxia in Lewis lung carcinoma

International Nuclear Information System (INIS)

Peng Fang; Wang Jin; Zou Yi; Bao Yong; Huang Wenlin; Chen Guangming; Luo Xianrong; Chen Ming

2011-01-01

Objective: To investigate whether recombinant human endostatin can create a time window of vascular normalization prior to vascular pruning to alleviate hypoxia in Lewis lung carcinoma in mice. Methods: Kinetic changes in morphology of tumor vasculature in response to recombinant human endostatin were detected under a confocal microscope with immunofluorescent staining in Lewis lung carcinomas in mice. The hypoxic cell fraction of different time was assessed with immunohistochemical staining . Effects on tumor growth were monitored as indicated in the growth curve of tumors . Results: Compared with the control group vascularity of the tumors was reduced over time by recombinant human endostatin treatment and significantly regressed for 9 days. During the treatment, pericyte coverage increased at day 3, increased markedly at day 5, and fell again at day 7. The vascular basement membrane was thin and closely associated with endothelial cells after recombinant human endostatin treatment, but appeared thickened, loosely associated with endothelial cells in control tumors. The decrease in hypoxic cell fraction at day 5 after treatment was also found. Tumor growth was not accelerated 5 days after recombinant human endostatin treatment. Conclusions: Recombinant human endostatin can normalize tumor vasculature within day 3 to 7, leading to improved tumor oxygenation. The results provide important experimental basis for combining recombinant human endostatin with radiation therapy in human tumors. (authors)

Lung density

DEFF Research Database (Denmark)

Garnett, E S; Webber, C E; Coates, G

1977-01-01

The density of a defined volume of the human lung can be measured in vivo by a new noninvasive technique. A beam of gamma-rays is directed at the lung and, by measuring the scattered gamma-rays, lung density is calculated. The density in the lower lobe of the right lung in normal man during quiet...... breathing in the sitting position ranged from 0.25 to 0.37 g.cm-3. Subnormal values were found in patients with emphsema. In patients with pulmonary congestion and edema, lung density values ranged from 0.33 to 0.93 g.cm-3. The lung density measurement correlated well with the findings in chest radiographs...... but the lung density values were more sensitive indices. This was particularly evident in serial observations of individual patients....

Study on the mechanism of the 3He+p→p+p+d reaction at 3He momentum of 5 GeV/c

International Nuclear Information System (INIS)

Blinov, A.V.; Chuvilo, I.V.; Drobot, V.V.

1985-01-01

The mechanism of the reaction 3 He+p → p+p+d is studied making use of the ITEP 80-cm liquid-hydrogen bubble chamber exposed to the beam of 5 GeV/c 3 He nuclei. The reaction cross sections is equal to 20.6+-0.3 mb. The phase space regions associated with quasi-free scattering (QFS) and final state interaction (FSI) are selected. Angular, mass and momentum distributions of the reaction products are obtained in the entire kinematically allowed range. The experimental data in the QFS region are compared with theoretical calculations based on the simplest pole diagram approximation. The 3 He and deuteron wave functions (WF) correspond to the realistic Reid potential. The D-wave components of these WF are taken into account. The absolute value of the cross section and shape of the distributions are described as a whole reasonably enough in the frame of the considered model in the kinematical region where FSI may be neglected

Dissipation of flow in superfluid 3He-A

International Nuclear Information System (INIS)

Paalanen, M.A.; Osheroff, D.D.

1980-01-01

The first direct measurements of the dissipation due to flow are performed and the effects of that flow on the NMR properties of 3 He-A are studied. A temperature-independent critical velocity v/sub c/=0.52 mm/s is observed, above which a pressure difference proportional to v/sub s/-v/sub c/ developes across our flow orifice. simultaneously, the NMR frequency in 3 He-A is lowered by an amount also proportional to v/sub s/-v/sub c/, in contrast to current theoretical predictions

Global gene expression profiling reveals a suppressed immune response pathway associated with 3q amplification in squamous carcinoma of the lung

Directory of Open Access Journals (Sweden)

Jun Qian

2015-09-01

Full Text Available Chromosome 3q26–28 is a critical region of genomic amplification in non-small cell lung cancer (NSCLC, particularly lung squamous cell carcinomas (SCCs. No molecular therapeutic target has shown clinical utility for SCC, in contrast with adenocarcinomas of the lung. To identify novel candidate drivers in this region, we performed both Array Comparative Genomic Hybridization (array CGH, Agilent Human Genome CGH 244A oligo-microarrays and Gene Expression Microarray (Agilent Human Gene Expression 4 × 44 K microarray on 24 untreated lung SCC specimens. Using our previously published integrative genomics approach, we identified 12 top amplified driver genes within this region that are highly correlated and overexpressed in lung SCC. We further demonstrated one of the 12 top amplified driver Fragile X mental retardation-related protein 1 (FXR1 as a novel cancer gene in NSCLC and FXR1 executes its regulatory function by forming a novel complex with two other oncogenes, protein kinase C, iota ( PRKCI and epithelial cell transforming 2 (ECT2 within the same amplicon in lung cancer cell. Here we report that immune response pathways are significantly suppressed in lung SCC and negatively associated with 3q driver gene expression, implying a potential role of 3q drivers in cancer immune-surveillance. In light of the attractive immunotherapy strategy using blockade of negative regulators of T cell function for multiple human cancer including lung SCC, our findings may provide a rationale for targeting 3q drivers in combination of immunotherapies for human tumors harboring the 3q amplicon. The data have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE40089.

Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA).

Science.gov (United States)

Zhou, Lijun; Lv, Zhiqiang; Shao, Jing; Xu, Ying; Luo, Xiaohong; Zhang, Yuming; Hu, Yang; Zhang, Wenji; Luo, Shuhong; Fang, Jianmin; Wang, Ying; Duan, Chaohui; Huang, Ruopan

2016-09-01

The human epididymis protein 4 (HE4) may have high specificity in the detection of malignant diseases, making the development of an immunoassay for HE4 essential. In our study, a fusion gene was constructed encoded with the HE4 protein. This protein was then produced in the bacterial cells (Escherichia coli) and used to immunize mice in order to eventually generate hybridomas specific to HE4. The hybridoma supernatants were then screened, and four positive anti-HE4 cell lines were selected. These cell lines produce monoclonal antibodies against HE4 epitopes, as demonstrated in the Western blot as well as by direct enzyme-linked immunosorbent assay (ELISA). Using the developed antibodies, we successfully identified several good antibody pairs from the hybridomas, which allowed for the development of a sandwich ELISA to measure HE4 levels. By using the HE4 ELISA, we measured HE4 levels of 60 clinical human serum samples. Compared with the Food and Drug Administration (FDA) approved kit (Roche), our results showed a strong positive correlation to those of the FDA-approved kit. In summary, highly sensitive antibody pairs were screened against HE4, and a sandwich ELISA was developed as an accurate analytical tool for the detection of HE4 in human serum, which could be especially valuable for diagnosing ovarian carcinomas. © 2015 Wiley Periodicals, Inc.

Optimizing moderation of He-3 neutron detectors for shielded fission sources

Energy Technology Data Exchange (ETDEWEB)

Rees, Lawrence B., E-mail: Lawrence_Rees@byu.edu [Department of Physics and Astronomy, Brigham Young University, Provo, UT 84602 (United States); Czirr, J. Bart, E-mail: czirr@juno.com [Department of Physics and Astronomy, Brigham Young University, Provo, UT 84602 (United States)

2012-11-01

The response of a {sup 3}He neutron detector is highly dependent on the amount of moderator incorporated into the detector system. If there is too little moderation, neutrons will not react with the {sup 3}He. If there is too much moderation, neutrons will not reach the {sup 3}He. In applications for portal or border monitors where {sup 3}He detectors are used to interdict illicit importation of plutonium, the fission source is always shielded to some extent. Since the energy distribution of neutrons emitted from the source depends on the amount and type of shielding present, the optimum placement of moderating material around {sup 3}He tubes is a function of shielding. In this paper, we use Monte Carlo techniques to model the response of {sup 3}He tubes placed in polyethylene boxes for moderation. To model the shielded fission neutron source, we use a point {sup 252}Cf source placed in the center of polyethylene spheres of varying radius. Detector efficiency as a function of box geometry and shielding is explored. We find that increasing the amount of moderator behind and to the sides of the detector generally improves the detector response, but that incremental benefits are minimal if the thickness of the polyethylene moderator is greater than about 5-7 cm. The thickness of the moderator in front of the {sup 3}He tubes, however, is very important. For bare sources, about 4-5 cm of moderator is optimum, but as the shielding increases, the optimum thickness of this moderator decreases to 0.5-1 cm. Similar conclusions can be applied to polyethylene boxes employing two {sup 3}He tubes. Two-tube boxes with front moderators of non-uniform thickness may be useful for detecting neutrons over a wide energy range.

Lung Cancer and Human Papilloma Viruses (HPVs: Examining the Molecular Evidence

Directory of Open Access Journals (Sweden)

Priya R. Prabhu

2012-01-01

Full Text Available Human papilloma virus (HPV, known to be an etiological agent for genital cancers, has been suggested also to be a possible contributory agent for lung cancer. Alternatively, lung cancer, formerly considered to be solely a smoker's disease, may now be more appropriately categorised into never smoker's and smoker's lung cancer. Through this paper we attempt to bring forth the current knowledge regarding mechanisms of HPV gaining access into the lung tissue, various strategies involved in HPV-associated tumorigenesis in lung tissue.

Results on Double-polarization Asymmetries in Quasielastic Scattering from Polarized 3He

Directory of Open Access Journals (Sweden)

Sulkosky Vincent A.

2016-01-01

Full Text Available The 3He nucleus has become extremely important in the investigation of the neutron’s spin structure. When polarized, 3He acts as an effective polarized neutron target and hence facilitates our understanding of the neutron’s internal structure. However, to be used in this manner, our understanding of the internal structure of 3He is of extreme importance. As the precision of experiments has improved, the extraction of polarized neutron information from 3He leads to an ever larger share of the systematic uncertainty for these experiments. In these proceedings, I present a precise measurement of beam-target asymmetries in the He→3(e→ ,e′d${}^3\\overrightarrow {He} (\\vec e,e'd$ and He→3(e→,e′p${}^3\\overrightarrow {He} (\\vec e,e'p$ reactions. The former process is a uniquely sensitive probe of hadron dynamics in 3He and the structure of the underlying electromagnetic currents. The measurements have been performed around the quasi-elastic peak at Q2 = 0.25 (GeV/c2 and 0.35 (GeV/c2 for recoil momenta up to 270 MeV/c. The experimental apparatus, analysis and results were presented together with a comparison to state-of-the art Faddeev calculations.

Insulin utilizes the PI 3-kinase pathway to inhibit SP-A gene expression in lung epithelial cells

Directory of Open Access Journals (Sweden)

Snyder Jeanne M

2002-10-01

Full Text Available Abstract Background It has been proposed that high insulin levels may cause delayed lung development in the fetuses of diabetic mothers. A key event in lung development is the production of adequate amounts of pulmonary surfactant. Insulin inhibits the expression of surfactant protein A (SP-A, the major surfactant-associated protein, in lung epithelial cells. In the present study, we investigated the signal transduction pathways involved in insulin inhibition of SP-A gene expression. Methods H441 cells, a human lung adenocarcinoma cell line, or human fetal lung explants were incubated with or without insulin. Transcription run-on assays were used to determine SP-A gene transcription rates. Northern blot analysis was used to examine the effect of various signal transduction inhibitors on SP-A gene expression. Immunoblot analysis was used to evaluate the levels and phosphorylation states of signal transduction protein kinases. Results Insulin decreased SP-A gene transcription in human lung epithelial cells within 1 hour. Insulin did not affect p44/42 mitogen-activated protein kinase (MAPK phosphorylation and the insulin inhibition of SP-A mRNA levels was not affected by PD98059, an inhibitor of the p44/42 MAPK pathway. In contrast, insulin increased p70 S6 kinase Thr389 phosphorylation within 15 minutes. Wortmannin or LY294002, both inhibitors of phosphatidylinositol 3-kinase (PI 3-kinase, or rapamycin, an inhibitor of the activation of p70 S6 kinase, a downstream effector in the PI 3-kinase pathway, abolished or attenuated the insulin-induced inhibition of SP-A mRNA levels. Conclusion Insulin inhibition of SP-A gene expression in lung epithelial cells probably occurs via the rapamycin-sensitive PI 3-kinase signaling pathway.

Human pericytes adopt myofibroblast properties in the microenvironment of the IPF lung.

Science.gov (United States)

Sava, Parid; Ramanathan, Anand; Dobronyi, Amelia; Peng, Xueyan; Sun, Huanxing; Ledesma-Mendoza, Adrian; Herzog, Erica L; Gonzalez, Anjelica L

2017-12-21

Idiopathic pulmonary fibrosis (IPF) is a fatal disease of unknown etiology characterized by a compositionally and mechanically altered extracellular matrix. Poor understanding of the origin of α-smooth muscle actin (α-SMA) expressing myofibroblasts has hindered curative therapies. Though proposed as a source of myofibroblasts in mammalian tissues, identification of microvascular pericytes (PC) as contributors to α-SMA-expressing populations in human IPF and the mechanisms driving this accumulation remain unexplored. Here, we demonstrate enhanced detection of α-SMA+ cells coexpressing the PC marker neural/glial antigen 2 in the human IPF lung. Isolated human PC cultured on decellularized IPF lung matrices adopt expression of α-SMA, demonstrating that these cells undergo phenotypic transition in response to direct contact with the extracellular matrix (ECM) of the fibrotic human lung. Using potentially novel human lung-conjugated hydrogels with tunable mechanical properties, we decoupled PC responses to matrix composition and stiffness to show that α-SMA+ PC accumulate in a mechanosensitive manner independent of matrix composition. PC activated with TGF-β1 remodel the normal lung matrix, increasing tissue stiffness to facilitate the emergence of α-SMA+ PC via MKL-1/MTRFA mechanotranduction. Nintedanib, a tyrosine-kinase inhibitor approved for IPF treatment, restores the elastic modulus of fibrotic lung matrices to reverse the α-SMA+ phenotype. This work furthers our understanding of the role that microvascular PC play in the evolution of IPF, describes the creation of an ex vivo platform that advances the study of fibrosis, and presents a potentially novel mode of action for a commonly used antifibrotic therapy that has great relevance for human disease.

3He release characteristics of metal tritides and scandium--tritium solid solutions

International Nuclear Information System (INIS)

Perkins, W.G.; Kass, W.J.; Beavis, L.C.

1976-01-01

Tritides of such metals as Sc, Ti, and Er are useful materials for determining the effects of He accumulation in metallic solids, for example, CTR first wall materials. Such effects include lattice strain and gross deformation which are related to 3 He retention and ultimate release. Long term gas release studies have indicated that, during the early life of a metal ditritide, a large fraction of the 3 He is retained in the solid. At more advanced ages, the 3 He release rate becomes comparable to the generation rate. Statistical analysis of the data indicates that the acceleration in 3 He release rate depends on accumulated 3 He concentration rather than strictly on age. 3 He outgassing results are presented for thin films of ScT 2 , TiT 2 , and ErT 2 , and the critical 3 He concentrations are discussed in terms of a percolation model. Phase transformations which occur on tritide formation cast some doubt on the validity of extrapolating results obtained for metal tritides to predictions regarding the accumulation of helium in metals. Sc is unique among the early transition and rare-earth metals in that the metal exhibits a very high room temperature T solubility (T/Sc = 0.4) with no phase transformation. Indeed, even the lattice parameters of the hcp Sc lattice are only minimally changed by T solution. Single crystal ScT/sub 0.3/ samples in two crystallographic orientations were obtained. Using a very sensitive technique, 3 He emission was measured from both these samples, as well as from fine-grained thin film Sc--T solid solution samples (ScT/sub 0.3-0.4/). The fine-grained film samples release 3 He at 2-3 percent of the generation rate, while the emission rate from the single-crystal samples is approximately 0.05 percent of the generation rate, indicating a strong grain size effect

Hyperfine Structure Measurements of Antiprotonic $^3$He using Microwave Spectroscopy

CERN Document Server

Friedreich, Susanne

The goal of this project was to measure the hyperfine structure of $\\overline{\\text{p}}^3$He$^+$ using the technique of laser-microwave-laser spectroscopy. Antiprotonic helium ($\\overline{\\text{p}}$He$^+$) is a neutral exotic atom, consisting of a helium nucleus, an electron and an antiproton. The interactions of the angular momenta of its constituents cause a hyperfine splitting ({HFS}) within the energy states of this new atom. The 3\\% of formed antiprotonic helium atoms which remain in a metastable, radiative decay-dominated state have a lifetime of about 1-3~$\\mu$s. This time window is used to do spectroscopic studies. The hyperfine structure of $\\overline{\\text{p}}^4$He$^+$ was already extensively investigated before. From these measurements the spin magnetic moment of the antiproton can be determined. A comparison of the result to the proton magnetic moment provides a test of {CPT} invariance. Due to its higher complexity the new exotic three-body system of $\\overline{\\text{p}}^3$He$^+$ is a cross-check...

Toona Sinensis Extracts Induced Cell Cycle Arrest and Apoptosis in the Human Lung Large Cell Carcinoma

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Cheng-Yuan Wang

2010-02-01

Full Text Available Toona sinensis extracts have been shown to exhibit anti-cancer effects in human ovarian cancer cell lines, human promyelocytic leukemia cells and human lung adenocarcinoma. Its safety has also been confirmed in animal studies. However, its anti-cancer properties in human lung large cell carcinoma have not been studied. Here, we used a powder obtained by freeze-drying the super-natant of centrifuged crude extract from Toona sinensis leaves (TSL-1 to treat the human lung carcinoma cell line H661. Cell viability was evaluated by the 3-(4-,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide assay. Flow cytometry analysis revealed that TSL-1 blocked H661 cell cycle progression. Western blot analysis showed decreased expression of cell cycle proteins that promote cell cycle progression, including cyclin-dependent kinase 4 and cyclin D1, and increased the expression of proteins that inhibit cell cycle progression, including p27. Furthermore, flow cytometry analysis showed that TSL-1 induced H661 cell apoptosis. Western blot analysis showed that TSL-1 reduced the expression of the anti-apoptotic protein B-cell lymphoma 2, and degraded the DNA repair protein, poly(ADP-ribose polymerase. TSL-1 shows potential as a novel therapeutic agent or for use as an adjuvant for treating human lung large cell carcinoma.

The ground state energy of 3He droplet in the LOCV framework

International Nuclear Information System (INIS)

Modarres, M.; Motahari, S.; Rajabi, A.

2012-01-01

The (extended) lowest order constrained variational method was used to calculate the ground state energy of liquid helium 3 ( 3 He) droplets at zero temperature. Different types of density distribution profiles, such as the Gaussian, the Quasi-Gaussian and the Woods-Saxon were used. It was shown that at least, on average, near 20 3 He atoms are needed to get the bound state for 3 He liquid droplet. Depending on the choice of the density profiles and the atomic radius of 3 He, the above estimate can increase to 300. Our calculated ground state energy and the number of atoms in liquid 3 He droplet were compared with those of Variational Monte Carlo method, Diffusion Monte Carlo method and Density Functional Theory, for which a reasonable agreement was found.

Cellular morphometry of the bronchi of human and dog lungs

International Nuclear Information System (INIS)

Robbins, E.S.

1991-09-01

One hundred and forty-seven bronchial samples (generations 3--6) from 66 patients (62 usable; 36 female, 26 male; median age 61) have been dissected by generation from fixed surgical lung specimens obtained after the removal of pathological lesions. In addition, one hundred and fifty-six mongol dog bronchi (generations 2--6) dissected from different lobes of 26 dog lungs have also been similarly prepared. One hundred and twenty-seven human samples have been completely processed for electron microscopy and have yielded 994 electron micrographs of which 655 have been entered into the Computerized Stereological Analysis System (COSAS) and been used for the measurement of the distances of basal and mucous cell nuclei to the epithelial free surface. Similarly 328 micrographs of dog epithelium from 33 bronchial samples have been used to measure the distances of basal and mucous cell nuclei to the epithelial free surface and have been entered into COSAS. Using the COSAS planimetry program, we continue to expand our established data bases which describe the volume density and nuclear numbers per electron micrograph for 5 cell types of the human bronchial epithelial lining of men and women, as well as smokers, non-smokers and ex-smokers and similar parameters for the same 5 epithelial cell types of dog bronchi. Our micrographs of human bronchial epithelium have allowed us to analyze the recent suggestion that the DNA of lymphocytes may be subject to significant damage from Rn progeny while within the lung. Since the last progress report three papers have been submitted for publication. 17 refs., 4 tabs

The 3He spectral function in light-front dynamics

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Rinaldi Matteo

2016-01-01

Full Text Available A distorted spin-dependent spectral function for 3He is considered for the extraction of the transverse-momentum dependent parton distributions in the neutron from semi-inclusive deep inelastic electron scattering off polarized 3He at finite momentum transfers, where final state interactions are taken into account. The generalization of the analysis to a Poincaré covariant framework within the light-front dynamics is outlined.

Pionic annihilation of antiprotons stopped on sup 3 He

Energy Technology Data Exchange (ETDEWEB)

Bendiscioli, G.; Filippini, V.; Rotondi, A.; Salvini, P.; Venaglioni, A. (Pavia Univ. (Italy). Dipt. di Fisica Nucleare e Teorica Istituto Nazionale di Fisica Nucleare, Pavia (Italy)); Bossolasco, S.; Bussa, M.P.; Busso, L.; Fava, L.; Ferrero, L.; Maggiora, A.; Panzieri, D.; Piragino, G.; Piragino, R.; Tosello, F. (Turin Univ. (Italy). Ist. di Fisica Generale Istituto Nazionale di Fisica Nucleare, Turin (Italy)); Batusov, Yu.A.; Falomkin, I.V.; Pontecorvo, G.B.; Rozhdestvensky, A.M.; Sapozhnikov, M.G.; Tretyak, V.I. (Joint Inst. for Nuclear Research, Dubna (USSR)); Guaraldo, C. (Istituto Nazionale di Fisica Nucleare, Frascati (Italy). Lab. Nazionale di Frascati); Lodi Rizzini, E. (Brescia Univ. (Italy). Dipt. di Automazione Industriale Istituto Nazionale di Fisica Nucleare, Turin (Italy)); Haatuft, A.; Halsteinslid, A.; Myklebost, K.; Olsen, J.M. (Bergen Univ. (Norway). Dept. of Physics); Breivik, F.O.; Jacobsen, T.; Soerensen, S.O. (Oslo Univ. (Norway). Physics Dept.); Balestra

1990-11-26

The pionic annihilation of antiprotons stopped on {sup 3}He nuclei in a self-shunted streamer chamber exposed to the antiproton beam of LEAR is studied. The data concern charged-particle multiplicity distributions, branching ratios for different final states, the probability of final-state interaction, {pi}{sup -}, {pi}{sup +}, p and d momentum spectra, like and unlike pion angular correlations, {pi} and other charged-particle angular correlations. The comparison of the {sup 3}He data with those obtained on {sup 1}H, {sup 2}H and {sup 4}He does not reveal relevant effects due to the increase of the nucleon number; the small differences can be seen as due to a weak final-state interaction. (orig.).

Theoretical charge-exchange total cross sections for B+3 + He and C+4 + He collisions

International Nuclear Information System (INIS)

Shipsey, E.J.; Browne, J.C.; Olson, R.E.

1977-01-01

Charge-exchange total cross sections for the B +3 + He and C +4 + He systems have been calculated in the velocity range v = (1--10) x 10 7 cm/sec. Ab initio potential-energy curves and coupling-matrix elements were computed and employed in the impact-parameter classical-coupled equations that describe the collision to obtain the cross sections. For the B +3 + He system, our calculations are in excellent agreement with experimental results with the finding that the single-electron-transfer cross section rises rapidly to a maximum of 1.45 x 10 -15 cm 2 at v = 5.5 x 10 7 cm/sec. For the C +4 + He system, however, we find that the double-electron-transfer process is more important than the single-electron-transfer process. For example, at v = 5 x 10 7 cm/sec, the double-electron-transfer cross section is found to be 0.6 x 10 -16 cm 2 vs 5.5 x 10 -17 for the single-electron transfer. This is in disagreement with an experiment of Crandall which gave the single-charge-transfer process as dominant. However, the more recent experiment reported by Crandall, Olson, Browne, and Shipsey verifies the double charge transfer as the dominant process for low energies

[Primitive lung abscess: an unusual situation in children].

Science.gov (United States)

Bouyahia, O; Jlidi, S; Sammoud, A

2014-12-01

Lung abscess is a localized area of non tuberculosis suppurative necrosis of the parenchyma lung, resulting in formation of a cavity containing purulent material. This pathology is uncommon in childhood. A 3-year-6 month-old boy was admitted with prolonged fever and dyspnea. Chest X-ray showed a non systemized, well limited, thick walled, hydric, and excavated opacity containing an air-fluid level. Chest ultrasound examination showed a collection of 6. 8 cm of diameter in the right pulmonary field with an air-fluid level. Hemoculture showed Staphylococcus aureus. The patient received large spectrum antibiotherapy. Three days after, he presented a septic shock and surgical drainage was indicated. Histological examination confirmed the diagnosis of lung abscess. Any underlying condition such as inoculation site, local cause or immune deficiency, was noted and diagnosis of primary abscess was made. The patient demonstrated complete recovery. He is asymptomatic with normal chest X-ray and pulmonary function after 3 years of evolution. Lung abscess represent a rare cause of prolonged fever in childhood. An underlying condition must be excluded to eliminate secondary abscess. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

Production of e(+)e(-) pairs in proton-deuteron capture to He-3

NARCIS (Netherlands)

Korchin, AY; Van Neck, D; Waroquier, M; Scholten, O; Dieperink, AEL

1998-01-01

The process p + d He-3 + gamma * at intermediate energies is described using a covariant and gauge-invariant model, and a realistic pd(3)He vertex, Both photodisintegration of He-3 and proton-deuteron capture with production of e(+)e(-) pairs are studied, and results for cross sections and response

Towards the observation of the magnetic structure of solid 3He

International Nuclear Information System (INIS)

Rufin, D.

1982-03-01

Experiments involving neutron scattering by 3 He for which thermalization problems have been solved, along with the development of novel measurement techniques (density, magnetization, temperature) by polarized neutron transmission, crystallization experiments with 3 He and 4 He: a single crystal of helium have been obtained within a sintered metal in which the pore size is less than a micron are presented [fr

Preconceptual engineering design for the APT 3He Target/Blanket concept

International Nuclear Information System (INIS)

Mensink, D.L.

1994-01-01

A preconceptual engineering design has been developed for the 3 He Target/Blanket (T/B) System for the Accelerator Production of Tritium Project. This concept uses an array of pressure tubes containing tungsten rods for the neutron spallation source and 3 He gas contained in a metal tank and blanket tubes as the tritium production material. The engineering design is based on a physics model optimized for efficient tritium production. Principle engineering consideration were: provisions for cooling all materials including the 3 He gas; containment of the gas and radionuclides; remote handling; material compatibility; minimization of 3 He, D 2 O, and activated waste; modularity; and manufacturability. The design provides a basis for estimating the cost to implement the system

Phase transition of two-dimensional 3He from a dilute to a dense phase

International Nuclear Information System (INIS)

Bhattacharyya, B.K.; Gasparini, F.M.

1985-01-01

We have measured the heat capacity of 3 He in films of 4 He as thin as 10 A formed on a Nuclepore filter substrate. At low temperatures, where the 3 He is in the lowest state as far as motion perpendicular to the film surface, we find that the 3 He undergoes a transition from a dilute phase to a dense phase. We have observed this transition for films of 4 He of 12.3- and 10-A thickness and for coverages of 3 He below about 0.2 atomic layers. For thicker 4 He films, and higher 3 He coverages, the 3 He remains homogeneously spread out over the surface of the 4 He. The striking characteristic of the transition is the sudden onset of linear temperature dependence with a slope which is proportional to the amount of 3 He in the calorimeter. This is consistent with the formation of islands of a dense two-dimensional phase which grows in extent proportionately to the amount of 3 He. Two puzzling aspects of our results are the lack of a significant heat-capacity jump at the transition and, based on a linear extrapolation of the data to zero temperature, a substantial amount of missing entropy

Mycobacterium tuberculosis Invasion of the Human Lung: First Contact

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Jeroen Maertzdorf

2018-06-01

Full Text Available Early immune responses to Mycobacterium tuberculosis (Mtb invasion of the human lung play a decisive role in the outcome of infection, leading to either rapid clearance of the pathogen or stable infection. Despite their critical impact on health and disease, these early host–pathogen interactions at the primary site of infection are still poorly understood. In vitro studies cannot fully reflect the complexity of the lung architecture and its impact on host–pathogen interactions, while animal models have their own limitations. In this study, we have investigated the initial responses in human lung tissue explants to Mtb infection, focusing primarily on gene expression patterns in different tissue-resident cell types. As first cell types confronted with pathogens invading the lung, alveolar macrophages, and epithelial cells displayed rapid proinflammatory chemokine and cytokine responses to Mtb infection. Other tissue-resident innate cells like gamma/delta T cells, mucosal associated invariant T cells, and natural killer cells showed partially similar but weaker responses, with a high degree of variability across different donors. Finally, we investigated the responses of tissue-resident innate lymphoid cells to the inflammatory milieu induced by Mtb infection. Our infection model provides a unique approach toward host–pathogen interactions at the natural port of Mtb entry and site of its implantation, i.e., the human lung. Our data provide a first detailed insight into the early responses of different relevant pulmonary cells in the alveolar microenvironment to contact with Mtb. These results can form the basis for the identification of host markers that orchestrate early host defense and provide resistance or susceptibility to stable Mtb infection.

Study of the (3He,t) reaction on 48Ca at 82 MeV

International Nuclear Information System (INIS)

Tanabe, T.; Yasue, M.; Sato, K.; Soga, F.; Igarashi, M.; Ogino, K.; Kadota, Y.; Saito, Y.; Shimokoshi, F.

1978-01-01

The reactions ( 3 He,t), ( 3 He, 3 He), ( 3 He,α) and ( 3 He,d) on 48 Ca have been studied at an incident energy of 82 MeV. The obtained angular distributions for the ( 3 He,t) reaction have been analyzed in terms of second-order DWBA theory, using a zero-range approximation. The relative ratio of the cross sections for the excitation of the fsub(7/2)f -1 sub(7/2) multiplets of 48 Sc could not be explained consistently by the one-step charge-exchange process only. However, the ratio was reproduced in order of magnitude by second-order DWBA calculations including the effect of two-step processes, such as ( 3 He-α-t) and ( 3 He-d-t) channels. The effects of the d- and α-channels on the elastic scattering of 3 He from 48 Ca were also examined in the framework of second-order full finite-range DWBA. (Auth.)

Reciprocal activation of α5-nAChR and STAT3 in nicotine-induced human lung cancer cell proliferation.

Science.gov (United States)

Zhang, Yao; Jia, Yanfei; Li, Ping; Li, Huanjie; Xiao, Dongjie; Wang, Yunshan; Ma, Xiaoli

2017-07-20

Cigarette smoking is the top environmental risk factor for lung cancer. Nicotine, the addictive component of cigarettes, induces lung cancer cell proliferation, invasion and migration via the activation of nicotinic acetylcholine receptors (nAChRs). Genome-wide association studies (GWAS) show that CHRNA5 gene encoding α5-nAChR is especially relevant to lung cancer. However, the mechanism of this subunit in lung cancer is not clear. In the present study, we demonstrate that the expression of α5-nAChR is correlated with phosphorylated STAT3 (pSTAT3) expression, smoking history and lower survival of non-small cell lung cancer (NSCLC) samples. Nicotine increased the levels of α5-nAChR mRNA and protein in NSCLC cell lines and activated the JAK2/STAT3 signaling cascade. Nicotine-induced activation of JAK2/STAT3 signaling was inhibited by the silencing of α5-nAChR. Characterization of the CHRNA5 promoter revealed four STAT3-response elements. ChIP assays confirmed that the CHRNA5 promoter contains STAT3 binding sites. By silencing STAT3 expression, nicotine-induced upregulation of α5-nAChR was suppressed. Downregulation of α5-nAChR and/or STAT3 expression inhibited nicotine-induced lung cancer cell proliferation. These results suggest that there is a feedback loop between α5-nAChR and STAT3 that contributes to the nicotine-induced tumor cell proliferation, which indicates that α5-nAChR is an important therapeutic target involved in tobacco-associated lung carcinogenesis. Copyright © 2017 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights reserved.