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Sample records for hu weisong shi

  1. Hu Shi's Study of Chinese Medieval intellectual History

    Institute of Scientific and Technical Information of China (English)

    Lou Yulie

    2006-01-01

    Hu Shi frequently gave lectures on the history of Chinese philosophy,especially the history of ancient Chinese philosophy,from the year 1919 to 1937.A large number of papers and dissertations published during this period are related to his research on this topic.In his opinion,there are three characteristics of the history of ancient Chinese philosophy:" religionalization of thought," "Indianization of philosophy," and "conflict between Chinese thought and Indian thought." In this paper,I explore Hu Shi's deep insight into the religionalization of Confucianism in Han dynasty and into the thought of Taoism in the medieval times.

  2. Hu Shi and the Continuation of the Revolution of1911——Discussing Intellectuals and Social Revolution%Hu Shi and the Continuation of the Revolution of 1911——Discussing Intellectuals and Social Revolution

    Institute of Scientific and Technical Information of China (English)

    Zhu Cheng

    2011-01-01

    Hu Shi is regarded as one of the most important intellectuals after the Revolution of 1911,and he has a far- reaching influence in 20th century.In the perspective of Hu Shi,the contribution and continuation of the Revolution of 1911 has some inspiration for us to analyze the relationship between Chinese intellectuals and social revolution.

  3. Did Hu Shi Really Believe in Traditional Chinese Medicine%胡适对中医究竟持什么态度

    Institute of Scientific and Technical Information of China (English)

    祖述宪

    2001-01-01

    胡适是20世纪初中国新文化运动的领袖和思想家,但是,80年代后期以来,我国出版的有关他的生平的著作中,根据胡适所作的“题陆仲安秋室研经图”,认为中医陆仲安早年治愈过他的“糖尿病、慢性肾炎和心脏病”,因而断言胡适相信中医药。仔细检阅胡适的日记、书信和文章,可以得出完全不同的结论。尽管1920—1921年胡适生的那场病,开始时有医生怀疑可能是心脏病或肾炎,但很快就否定了。胡适虽然死于心肌梗塞,但他第一次冠心病发作是1938年底。实际上,中医陆仲安为他治疗的只不过是胡适所说的“小病”,是自限性的,而且胡适一再申明他根本不曾生过糖尿病和慢性肾炎。上述陆仲安治愈胡适糖尿病等疾病的说法纯属以讹传讹,或有意夸张造谣,这件事在中医界至今仍有影响。胡适自称是“信仰西医的人”,并且支持他的友人批评中医,这些批评在今天仍然具有重要的现实意义。%Dr.Hu Shi(or Hu Shih,1891—1962),pioneer of China's New CulturalMovement in the early 20th century,is not only a great thinker but also the leader of the Vernacular Movement and the subsequent literary revolution in China.His progressive and scientific attitude to academic research is epitomized by his advocacy of “dare to propose new hypotheses and then subject them to rigorous and methodical examination”.Several books published since the late 1980's claim that Hu Shi was a believer in traditional Chinese medicine(TCM).The basis for this assertion is his inscription of a painting titled “Lu Zhong-an Studying Medical Classics in Autumn” in 1921,which was done in gratitude after Hu Shi was cured by the practitioner of Chinese medicine Lu Zhong-an of diabetes,chronic nephritis and heart disease.However,a thorough review of Hu Shi's diary,letters and articles reveals that though he suffered his first heart attack in

  4. 鲁迅与胡适婚恋观的异同评价——兼与谢泳先生商榷%Why Mr. Lu Xun and Mr. Hu Shi had Different Understandings in Marriages

    Institute of Scientific and Technical Information of China (English)

    姚维荣

    2011-01-01

    鲁迅与胡适同为二十世纪的文化大师,同是封建包办婚姻的受害者,但他们对待无爱婚姻的态度却迥然相异,由此影响制约了他们婚外爱情的不同结局。鲁迅与胡适不同的婚恋观引发了人们不同的评价。%Both Lu Xun and Hu Shi are masters in the field of contemporary Culture in China. They both suffered a lot from the marriage arranged by their parents. But they had different understanding of it. So, when they met the love out of their families, they had got differ- ent results, which led to the comments by later people.

  5. “展缓判断”求真相:冯友兰与胡适对孔老先后之争的学术意义--兼对冯友兰复胡适信函时间考证%Suspension of Judgement:The Academic Significance of Debate Between Feng Youlan and Hu Shi ---Textual Research on Time of Feng Youlan and Hu Shi’s Letters

    Institute of Scientific and Technical Information of China (English)

    张婷

    2014-01-01

    Early 30s of the last century, Feng Youlan and Hu Shi had several debates on Confucius and Laozi. Each side held dif-ferent opinons from his own ideological analysis and textual research. After nearly half a century, finally under the support of the un-earthed cultural relics, Hu Shi was proved to be true. His“Suspension of judgment” academic principles on issues of the present academ-ic research has great significance.%20世纪30年代初,冯友兰与胡适等人针对老子其人其书与孔子的先后问题,曾经展开过多次交锋。双方各自从所擅长的思想系统分析和考证方法辨伪等几个方面进行讨论,意见迭出。近半个世纪后,最终在出土文物的佐证下,胡适生前的推断被证明为真。他所力倡的“展缓判断”学术原则及对争议问题的处理态度对当下的学术研究具有很大的启示意义。

  6. Convention and Method---An Inquiry of Hu Shi's Academic Ideas During the New Culture Movement%“家法”与“方法”--“新文化运动”时期胡适的学术观念考辨

    Institute of Scientific and Technical Information of China (English)

    李浴洋

    2015-01-01

    "Scientific method" was endorsed by Hu Shi throughout his life, but this does not mean that his definitions of its theoretic implications, ranges of application, even the hermeneutic method itself were consistent. In fact, it had multiple meanings changing with the different people he was addressing, the different time and situation he was in, as well as his different senses of time and position in the various stages of his life. Speaking of the New Culture Movement, his adjustment and dialectics of"convention (jiafa)" and "method (fangfa)" came to the fore. Hu Shi's discourse of his academic ideas is essentially a double construction of a new historical subject and subjectivity concerning historical narration.%胡适一生推崇“科学方法”,但对其理论内涵与适用范围的界定,甚至阐释方式本身,都并非一成不变,而是根据发言对象、时代诉求以及胡适在各个阶段的位置感与历史感的差异而有所不同。具体到“新文化运动”时期,便首先体现为他对于“家法”与“方法”的调适与辩证。胡适对于其学术观念的表达,在本质上是一种新的历史主体与主体性的历史叙述的双重建构。

  7. The Research on the Relations between Shi Xiao-ying’LUO HU YE LU and YUN WO JI TAN and Their Historical Value%论释晓莹《罗湖野录》与《云卧纪谈》的关系及史料价值

    Institute of Scientific and Technical Information of China (English)

    金建锋

    2015-01-01

    Shi Xiao-ying was a big important historian in the Chinese Buddhism history,who had two important Bud-dhism works———LUO HU YE LU and YUN WO JI TAN. The two works had some relations,in which YUN WO JI TA was based on LUO HU YE LU. The two works had some historical values in the Buddhism history,literature history and histori-cal history.%释晓莹是我国佛教史上具有一定影响的佛教史家,现存有两部重要的佛教著作———《罗湖野录》和《云卧纪谈》。两书的关系为:《云卧纪谈》应是《罗湖野录》的续作。两书对佛教史、文学史、史学史研究等具有一定的史料价值。

  8. Research on Diaries of Modern Chinese Writers: Examples of Hu Shi, Lu Xun and Yu Dafu%现代作家日记研究——以胡适、鲁迅、郁达夫为例

    Institute of Scientific and Technical Information of China (English)

    桑逢康

    2012-01-01

    在现代作家之中,胡适的日记写得最多最勤也最连贯(中间只有少量的缺失),总计约在350万字左右。胡适日记的特点是:(1)丰富性,个人经历包括思想演变、社会现象包括重大事件、人际关系等等,无不尽记其中;(2)准确性,即可作为一部信史;(3)以思想札记与叙事为主,某些篇章又有一定的抒情意味。鲁迅的日记以记事为主,而且大都很简略。虽无个人情感的抒发与内心世界的展示,但它对研究鲁迅的行止提供了重要的线索和依据。由于记述简略,所以又必须将日记中的点点滴滴与鲁迅的其他文章,以及他人的记载结合起来,对某件事情方能有一个比较完整的认识。郁达夫日记是真正意义上的“日记文学”,也是作者的“生活记录”。郁达夫本着固有的真率态度,“全部揭开在大家的眼前”,为人们研究郁达夫提供了最可宝贵的未曾涂改的第一手资料。他又是用散文笔法来写日记的,许多日记摘录出来就是一篇篇绝佳的散文作品,既有议论更有情节有细节有心理活动,情景交融,形象生动,人物的音容笑貌跃然纸上。%Among modem Chinese writers, Dr. Hu Shi( 1891 -1962) ranks no doubt the most prolific writer who had approximately completed a total amount of three millions and a half Chinese diary words in a successive way with merely few absences. His diaries feature in three aspects : ( 1 ) Richness in his personal experience including transformation of mind, social phenomena, significant events and human relationships, etc. ; (2) Accuracy in his retrospect over the past years that could be regarded even as a historical book; (3) Lyricism in his story-teller' s manner and philosophical essays. Diaries of Lu Xun (1881 - 1936) mainly fo- cused on details of daily life and demonstrated a simple genre of language. Although Lu Xun

  9. “Meticulous Crafting” and “Plain Style”: Hu Shi's Prose

    Institute of Scientific and Technical Information of China (English)

    ChenPingyuan; ZhuShida; DavidKelly

    2004-01-01

    Hu Shi's Outline History of Chinese Philosophy, despite a number of shortcomings, opened a new era for an academic prose in vernacular Chinese. Hu set great store by the structural features of a """"work,"""" arguing that it was not enough to assemble quotes,

  10. 论西方社会学批评理论对“五四”文学批评的影响--以茅盾、胡适、成仿吾为例%On the Influence of Western Sociological Theory of“May Fourth” Literary Criticism---Take Mao Dun, Hu Shi, Cheng Fangwu for Example

    Institute of Scientific and Technical Information of China (English)

    宋向红

    2015-01-01

    The method of literature research from the perspective of sociology is called sociology criticism.Western sociology criticism theories spread rapidly in China in“May Fourth Period”, had a great influence on literary criticism.It contributed to the establishment of sociological criticism genre in the field of the Chinese literary criticism, promoted the transformation of Chi-nese traditional literary criticism methods, made up for the deficiency of the aesthetic criticism to ignore social reality.Mao Dun was influenced by Turner’ s“three elements”, established the basic mode of Chinese social criticism.Hu Shi successfully fused Dewey’s pragmatism method and traditional academic research methods.Cheng Fangwu referred Guyan’s and Spencer’s social doctrine to formed“social-aesthetic” style criticism.%从社会学的角度研究文学的方法被学术界称之为社会学批评。五四时期西方社会学批评理论在中国迅速传播,对文学批评产生了巨大影响。它促成中国文艺批评领域中社会学批评流派的确立,推动中国传统文学批评方法的转型,弥补了审美批评忽视社会现实的不足。其中,茅盾受泰纳的“三要素”说的影响,确立了中国社会学批评的基本模式;胡适成功地将杜威的实用主义方法与传统治学方法融会贯通;成仿吾借鉴居友与格罗塞的社会学说,形成“社会—审美”的批评风格。

  11. Shi Xinning: Reinventing Reality

    Institute of Scientific and Technical Information of China (English)

    Jade Franklin

    2007-01-01

    <正>■In order to paint in a photorealist style there must first be a photograph from which to amass visual stimulus; this is the very nature of the genre. In the work of Shi Xinning, however, the conventional photorealist working process undergoes something of a conceptual overhaul, for instead of meticulously

  12. Causal Attributions of Shy Subjects.

    Science.gov (United States)

    Teglasi, Hedwig; Hoffman, Mary Ann

    1982-01-01

    Causal attributions of shy students (N=36) were compared with those of a comparison group of students (N=36) in ten situations. Significant differences between the two groups emerged when explaining outcomes of situations considered to be problematic for shy individuals. Causal attributions may reflect realistic and situation-specific…

  13. Shi Changxu, former vice president of NSFC wins Top Prize of National Science and Technology Award of China%Shi Changxu, former vice president of NSFC wins Top Prize of National Science and Technology Award of China

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Professor Shi Changxu, former vice-president of NSFC, won the Top Prize of National Science and Technology Award of China for 2011. At a ceremony at the Great Hall of the People in Beijing, he reccived the certificate from Chinese President Hu Jintao on January 14, 2011.

  14. Bijections for the Shi and Ish arrangements

    OpenAIRE

    Leven, Emily; Rhoades, Brendon; Wilson, Andrew Timothy

    2013-01-01

    The {\\sf Shi hyperplane arrangement} Shi(n) was introduced by Shi to study the Kazhdan-Lusztig cellular structure of the affine symmetric group. The {\\sf Ish hyperplane arrangement} Ish(n) was introduced by Armstrong in the study of diagonal harmonics. Armstrong and Rhoades discovered a deep combinatorial similarity between the Shi and Ish arrangements. We solve a collection of problems posed by Armstrong and Armstrong-Rhoades by giving bijections between regions of Shi(n) and Ish(n) which pr...

  15. Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

    Directory of Open Access Journals (Sweden)

    Shuangjiao Ma

    2017-02-01

    Full Text Available Traditional She medicine is part of China’s cultural heritage and has become remarkably popular worldwide. The Shi-Liang tea is made from the processed leaves of Chimonanthus salicifolius S. Y. Hu and Chimonanthus zhejiangensis M. C. Liu. To ensure the safety and efficacy of Shi-Liang tea, we used DNA barcoding based on the internal transcribed spacer 2 (ITS2 of nuclear ribosomal DNA to distinguish the original plant sources of Shi-Liang tea from closely related species. All 71 ITS2 sequences were aligned by Clustal-W, and genetic distances were computed using MEGA 6.0 according to the Kimura 2-parameter model. The results indicated that the sequence lengths of ITS2 regions of the original plants of Shi-Liang tea and closely related species ranged from 256 bp to 260 bp. Interspecific genetic distances ranged from 0 to 0.078. The neighbor-joining (NJ tree showed that the original plants of Shi-Liang tea species can be easily differentiated from closely related species. Distinct molecular differences were found between the secondary structures of ITS2 sequences from Shi-Liang tea and closely related species. The results in the present investigation suggested that the ITS2 could be an effective DNA marker to identify the original plants of Shi-Liang tea and their closely related species. These DNA barcodes can greatly benefit the supervision of the commercial circulation of She medicine.

  16. SHI Changxu: China's Superalloy Hero

    Institute of Scientific and Technical Information of China (English)

    XIN Ling

    2011-01-01

    Prof.Shi Changxu (Chang-hsu Shih), laureate of the State Top Scientific and Technological Award for 2010, is regarded as a pioneer in the development of superalloys in China and a world-class master of materials science with excellent leadership and strategic insights.In the 1960s, he and his colleagues worked out the first generation air-cooled nickel-based superalloy turbine blades for domestic fighter aircrafts which greatly enhanced the aeroengines' performance.Via the effective control of trace elements, he developed the low segregation technology to reduce the segregation of superalloys, for which the International Union of Materials Research Societies honored him with the "Innovations in Real Materials Award" in 1998.He advocated and promoted the research and development of carbon fibers and magnesium alloys in China as well as the establishment of the Chinese Academy of Engineering.Today, 30 years after retirement, Prof.Shi still works every day in his office to read latest research results, write advisory proposals and meet young visitors to give them his best support.

  17. Women stereotypes in Shi Zhecun's short stories.

    Science.gov (United States)

    Rosenmeier, Christopher

    2010-01-01

    This article analyses the representation of women in two 1933 short story collections by Shi Zhecun: An Evening of Spring Rain and Exemplary Conduct of Virtuous Women. It discusses how the New Woman image was a site of contestation in Republican China, and argues that Shi Zhecun’s short stories contain four basic stereotypes: the enigmatic woman, the estranged wife, the prostitute, and the inhibited woman. Using these narratives of women and how they were perceived by men, Shi Zhecun deconstructed the New Woman image by subverting the various ways modernity was projected onto women.

  18. Dr. Shi Hanzhang's Experience in Treating Andropathy

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Dr. Shi Hanzhang (施汉章) of Dongzhimen Hospital affiliated to Beijing University of Traditional Chinese Medicine is experienced in both teaching and clinical practice, especially in treating andropathy, such as sexual disorder, infertility, and hyperplasia of the prostate. The author has the honour to follow him for study and really learned a lot from him. A brief introduction to Dr. Shi's way of thinking in treatment is as follows.

  19. Biases about research on HU Shi%胡适研究中的误区

    Institute of Scientific and Technical Information of China (English)

    周久凤; 徐明成

    2001-01-01

    继20世纪50年代中期批判胡适之后,80年代末至今,思想界、学术界兴起了研究胡适的风潮.遗憾的是,众多研究文章和著作几乎毫元例外地存在着各自的乃至共同的误区.

  20. Research group honored with Qiu Shi Prize

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ A research team, headed by CHEN Chuangtian from the CAS Technical Institute of Physics and Chemistry, XU Zuyan from the CAS Institute of Physics, and JIANG Minhua from Shandong University, has received the Qiu Shi Prize for Outstanding Science & Technology Team Achievement in 2007.

  1. Main: 1HU9 [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1HU9 大豆 Soybean Glycine max (L.) Merrill Seed Lipoxygenase-3 Name=Lox1.3; Synonyms=...2; CAA31664.1; ALT_SEQ.|EMBL; U50081; AAB41272.1; -.|PDB; 1HU9; X-ray; A=1-857.|PDB; 1IK3; X-ray; A=1-857.|P...WTSDTRALEAFKRFGNKLAQIENKLSERNNDEKLRNRCGPVQMPYTLLLPSSKEGLTFRGIPNSISI soybean_1HU9.jpg ...

  2. Dark matter at the SHiP experiment

    Directory of Open Access Journals (Sweden)

    Timiryasov Inar

    2016-01-01

    Full Text Available We study prospects of dark matter searches in the SHiP experiment. SHiP (Search for Hidden Particles is the recently proposed fixed target experiment which will exploit the high-intensity beam of 400 GeV protons from the CERN SPS. In addition to the hidden sector detector, SHiP will be equipped with the ντ detector, which presumably would be sensitive to dark matter particles. We describe appropriate production and detection channels and estimate SHiP’s sensitivity for a scalar dark matter coupled to the Standard model through the vector mediator.

  3. Elementary School Teachers' Reflections on Shy Children in the Classroom

    Science.gov (United States)

    Bosacki, Sandra Leanne; Coplan, Robert J.; Rose-Krasnor, Linda; Hughes, Kathleen

    2011-01-01

    This study explored teachers' perceptions of shy children in the classroom during the elementary school grades. Seven teachers (1 male, 6 female) from elementary schools located in geographically diverse areas of Canada participated in semistructured telephone interviews that explored their perceptions of and experiences with shy children in the…

  4. A Model Psychoeducation Group for Shy College Students.

    Science.gov (United States)

    Martin, Virginia; Thomas, M. Carolyn

    2000-01-01

    Considers the planning, organizing, and conducting of a proposed model for shy college students. Offers several recommendations for educators and counselors on early identification of shy students, the design of educational environments to enhance development, and further research to answer questions about efficacy of treatment modes. (Author/JDM)

  5. Dicty_cDB: SHI895 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available m3b: 0.00 m_ : 1.00 60.0 %: mitochondrial 20.0 %: nuclear 8.0 %: cytoplasmic 4.0 %: cytoskeletal 4.0 %: vacuolar 4.0 %: extracellula...r, including cell wall >> prediction for SHI895 is mit 5' end seq. ID SHI895F 5' en

  6. Infecciones en huéspedes inmunocomprometidos

    OpenAIRE

    Cuéllar Ponce de León, Luis Ernesto; Médico-Cirujano. Especialista en Enfermedades Infecciosas y Tropicales. Jefe del Servicio de Infectología. Instituto Nacional de Enfermedades Neoplásicas. Coordinador del Programa VIH/SIDA/TARGA-INEN. Profesor Asociado de Medicina de la Universidad Nacional Federico Villarreal. Lima.

    2013-01-01

    Las infecciones son causa frecuente de morbimortalidad en huéspedes inmunocomprometidos. La frecuencia y diversidad de infecciones en esta población dependerá del tipo de inmunocompromiso, tratamiento recibido y aspecto epidemiológico. Los huéspedes con neutropenia desarrollan infecciones por bacterias extracelulares no encapsuladas y hongos levaduriformes y filamentosos; el diagnóstico precoz y tratamiento oportuno de las mismas son considerados una emergencia por su severidad y gran mortali...

  7. 论胡适的"诗的经验主义"%On HU Shi's "Empiricism in Poetry"

    Institute of Scientific and Technical Information of China (English)

    杨四平

    2006-01-01

    胡适从实验的角度,在历史的文学进化论的指导下,大胆提出了"三事论" 和前后"八事论",最后形成了他的核心诗观"诗的经验主义",同时,指出了它的改良性质.

  8. A probabilistic Hu-Washizu variational principle

    Science.gov (United States)

    Liu, W. K.; Belytschko, T.; Besterfield, G. H.

    1987-01-01

    A Probabilistic Hu-Washizu Variational Principle (PHWVP) for the Probabilistic Finite Element Method (PFEM) is presented. This formulation is developed for both linear and nonlinear elasticity. The PHWVP allows incorporation of the probabilistic distributions for the constitutive law, compatibility condition, equilibrium, domain and boundary conditions into the PFEM. Thus, a complete probabilistic analysis can be performed where all aspects of the problem are treated as random variables and/or fields. The Hu-Washizu variational formulation is available in many conventional finite element codes thereby enabling the straightforward inclusion of the probabilistic features into present codes.

  9. For Hu Zhuanglin: A Prefatory Note

    Institute of Scientific and Technical Information of China (English)

    M. A. K. Halliday

    2012-01-01

    It is a very great pleasure to be able to congratulate Professor Hu Zhuanglin on the occasion of his eightieth birthday. It is also a time to remember that Professor Hu and I have known each other for 33 years, since the moment he arrived in Sydney, together with eight other Chinese scholars, to take part in a programme of studies arranged jointly by the Department of English and the Department of Linguistics at Sydney University. These first arrivals from China were people of some seniority,

  10. Hu on poolel teel / James Miles

    Index Scriptorium Estoniae

    Miles, James

    2006-01-01

    2007. aastal toimuv kommunistliku partei 17. kongress tähistab Hiina presidendi Hu Jintao jaoks oma võimuaja poole peale jõudmist, ees ootavad sellised väljakutsed nagu olümpiamängude korraldamine, valimiskeeris Taiwanis ning hullunud majanduse stabiliseerimine. Vt samas: Hiina avanemise pidu

  11. Hu on poolel teel / James Miles

    Index Scriptorium Estoniae

    Miles, James

    2006-01-01

    2007. aastal toimuv kommunistliku partei 17. kongress tähistab Hiina presidendi Hu Jintao jaoks oma võimuaja poole peale jõudmist, ees ootavad sellised väljakutsed nagu olümpiamängude korraldamine, valimiskeeris Taiwanis ning hullunud majanduse stabiliseerimine. Vt samas: Hiina avanemise pidu

  12. [Quintessence of academician SHI Xue-min's acupoint needling method].

    Science.gov (United States)

    Yang, Ming-Xing

    2008-10-01

    Under guidance of the traditional theories of acupuncture and modern knowledge of anatomy and in combination with his long-term clinical practice and clinical researches, academician SHI Xue-min formulates scien tific and strict acupuncture methods and corresponding quantitative requirement for the acupoints for treatment of stroke and relative diseases and symptoms, including patient's posture, doctor's posture, angle of needle insertion, manipulation, amount of arrival of qi, etc. Correct grasping these needling methods of acupoints is a key to learn and understand SHI Xue-min's Acupuncture Science and "Xingnao Kaiqiao" needling method and quantitative acu puncture manipulation theory. In this paper, SHI Xue-min's partial experiences on needling acupoints are introduced.

  13. Dicty_cDB: SHI721 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Homology vs DNA Score E Sequences producing significant alignments: (bits) Value ...some 7. 38 0.019 5 dna update 2006. 4.28 Homology vs Protein Score E Sequences producing significant alignments: (bits) Value...li*kgpyiisknyflnklkk--- Homology vs CSM-cDNA Score E Sequences producing signific...ant alignments: (bits) Value SHJ350 (SHJ350Q) /CSM/SH/SHJ3-C/SHJ350Q.Seq.d/ 101 2e-21 SHI721 (SHI721Q) /CSM/

  14. Hu Xiaoyu—an Outstanding Pupil

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    HU Xiaoyu, 11, is a fifth grader at the Beijing Hepingli No. 4 Primary School. She is talkative, cheerful, active and likes to laugh. Her teachers say she is well-behaved and excels in her studies. She is intelligent and always asks many questions both in and outside the classroom. She has been elected a "Three Good" student every year since she started school.

  15. Hu Qiaomu and Hu Feng Event%胡乔木与胡风案

    Institute of Scientific and Technical Information of China (English)

    张成洁

    2015-01-01

    It had a gradual upgrade process on a critique of Hu Feng's literary thoughts and a political settlement to Hu Feng.As the senior vice minister of the Propaganda Department of the Central Committee of the CPC,Hu Qiaomu played an important role on it.In October 1945,Hu Qiaomu still stayed in Chungking after Chungking Negotiations and counter-acted the effects of Hu Feng's literary thoughts by criticizing"subjectiveness".Public criticism to Hufeng launched by"popular literature series" was supported by Hu Qiaomuin in Hongkong in 1948.The first two years of the founding of new China,Hu Qiaomu talked with Hufeng for many times to solve his ideological problems.Hu Qiaomu also organized criticism of Hu Feng's literary thoughts throught the opportunity of Shuwu self-criticism;Hu Qiaomu first mentioned publicly"a small cabal of literary headed by Hufeng"In his giving editorial notes of Shuwu's article.Hu Feng's private let-ters to Shuwu were disclosed which directly led to Hu Feng event upgrading to"anti-party and anti-revolution"problems in the process of the great criticism of Hu Feng.After 1954,this matter had an indirect relationship with Hu Qiaomu's suggest of writing Hu Feng sectarianism.However,Hu Qiaomu disapproved to determine Hu Feng and the people impli-cating with Hu Feng as"Hufeng Anti-Party Clique".He gave a claim in this connection before Mao Zedong.In the past, people very paid attention to Zhouyang who had deep historical grievances with Hu Feng and ignored the role of Hu Qiao-mu in Hu Feng event.%对胡风文艺思想的批判和对胡风的政治清算有一个渐次升级的过程。其中,在对胡风文艺思想的批判中,胡乔木起了重要作用。1945年10月,胡乔木随毛泽东去重庆谈判后逗留重庆,借批《论主观》来清除胡风文艺思想的影响;1948年香港的《大众文艺丛刊》对胡风发起的公开批评,也得到了胡乔木的支持;新中国成立头两年,胡乔木多次奉命找胡风谈话

  16. A bright example for all of us- Professor Shi Changxu

    Institute of Scientific and Technical Information of China (English)

    Wei Gao

    2011-01-01

    Professor Shi Chanxu received the Top Scientists Award of China in early 2011. This is a very important event. As a materials scientist originally from China, I am very happy to hear this news. I see this award as a great encouragement, not only for people working in the field of Materials Science and Engineering, but also for all scientists and technologists in China.

  17. Ma Shi Wen Tong and its Theory of Language

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    <正>Ma Shi Wen Tong is a very influential book in the field of linguistics.Its theory of language is closely related to the social background of China at that time.The paper will deal with concept of language in this book from a cultural and historical perspective.

  18. Working with Shy or Withdrawn Students. ERIC Digest.

    Science.gov (United States)

    Brophy, Jere

    This ERIC digest focuses on students who are commonly described as shy (inhibited, lacking in confidence, socially anxious) or withdrawn (unresponsive, uncommunicative, or daydreaming). Symptoms of shyness or withdrawal may appear as part of the student's overall personality or as a situation-specific response to a particular stress factor.…

  19. Shy-Drager syndrome a case report with polysomnography

    Directory of Open Access Journals (Sweden)

    F. S. Aloe

    1990-06-01

    Full Text Available The authors report a case of Shy-Drager syndrome in a 53 year-old male patient. Autonomic failure was made evident by physical examiration as well as laboratory tests. A sleep recording showed decreased percentage of REM sleep and apneas of the central type. The possible mechanisms for this sleep disorder are discussed.

  20. Regulation of HuR by DNA Damage Response Kinases

    Directory of Open Access Journals (Sweden)

    Hyeon Ho Kim

    2010-01-01

    Full Text Available As many DNA-damaging conditions repress transcription, posttranscriptional processes critically influence gene expression during the genotoxic stress response. The RNA-binding protein HuR robustly influences gene expression following DNA damage. HuR function is controlled in two principal ways: (1 by mobilizing HuR from the nucleus to the cytoplasm, where it modulates the stability and translation of target mRNAs and (2 by altering its association with target mRNAs. Here, we review evidence that two main effectors of ataxia-telangiectasia-mutated/ATM- and Rad3-related (ATM/ATR, the checkpoint kinases Chk1 and Chk2, jointly influence HuR function. Chk1 affects HuR localization by phosphorylating (hence inactivating Cdk1, a kinase that phosphorylates HuR and thereby blocks HuR's cytoplasmic export. Chk2 modulates HuR binding to target mRNAs by phosphorylating HuR's RNA-recognition motifs (RRM1 and RRM2. We discuss how HuR phosphorylation by kinases including Chk1/Cdk1 and Chk2 impacts upon gene expression patterns, cell proliferation, and survival following genotoxic injury.

  1. Flexible DNA bending in HU-DNA cocrystal structures.

    Science.gov (United States)

    Swinger, Kerren K; Lemberg, Kathryn M; Zhang, Ying; Rice, Phoebe A

    2003-07-15

    HU and IHF are members of a family of prokaryotic proteins that interact with the DNA minor groove in a sequence-specific (IHF) or non-specific (HU) manner to induce and/or stabilize DNA bending. HU plays architectural roles in replication initiation, transcription regulation and site-specific recombination, and is associated with bacterial nucleoids. Cocrystal structures of Anabaena HU bound to DNA (1P71, 1P78, 1P51) reveal that while underlying proline intercalation and asymmetric charge neutralization mechanisms of DNA bending are similar for IHF and HU, HU stabilizes different DNA bend angles ( approximately 105-140 degrees ). The two bend angles within a single HU complex are not coplanar, and the resulting dihedral angle is consistent with negative supercoiling. Comparison of HU-DNA and IHF-DNA structures suggests that sharper bending is correlated with longer DNA binding sites and smaller dihedral angles. An HU-induced bend may be better modeled as a hinge, not a rigid bend. The ability to induce or stabilize varying bend angles is consistent with HU's role as an architectural cofactor in many different systems that may require differing geometries.

  2. HU-446 and HU-465, Derivatives of the Non-psychoactive Cannabinoid Cannabidiol, Decrease the Activation of Encephalitogenic T Cells.

    Science.gov (United States)

    Kozela, Ewa; Haj, Christeene; Hanuš, Lumir; Chourasia, Mukesh; Shurki, Avital; Juknat, Ana; Kaushansky, Nathali; Mechoulam, Raphael; Vogel, Zvi

    2016-01-01

    Cannabidiol (CBD), the non-psychoactive cannabinoid, has been previously shown by us to decrease peripheral inflammation and neuroinflammation in mouse experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS). Here we have studied the anti-inflammatory effects of newly synthesized derivatives of natural (-)-CBD ((-)-8,9-dihydro-7-hydroxy-CBD; HU-446) and of synthetic (+)-CBD ((+)-8,9-dihydro-7-hydroxy-CBD; HU-465) on activated myelin oligodendrocyte glycoprotein (MOG)35-55-specific mouse encephalitogenic T cells (T(MOG) ) driving EAE/MS-like pathologies. Binding assays followed by molecular modeling revealed that HU-446 has negligible affinity toward the cannabinoid CB1 and CB2 receptors while HU-465 binds to both CB1 and CB2 receptors at the high nanomolar concentrations (Ki = 76.7 ± 5.8 nm and 12.1 ± 2.3 nm, respectively). Both, HU-446 and HU-465, at 5 and 10 μm (but not at 0.1 and 1 μm), inhibited the MOG35-55-induced proliferation of autoreactive T(MOG) cells via CB1/CB2 receptor independent mechanisms. Moreover, both HU-446 and HU-465, at 5 and 10 μm, inhibited the release of IL-17, a key autoimmune cytokine, from MOG35-55-stimulated T(MOG) cells. These results suggest that HU-446 and HU-465 have anti-inflammatory potential in inflammatory and autoimmune diseases.

  3. Multiple defects in Escherichia coli mutants lacking HU protein.

    OpenAIRE

    Huisman, O; Faelen, M; Girard, D; Jaffé, A; Toussaint, A; Rouvière-Yaniv, J

    1989-01-01

    The HU protein isolated from Escherichia coli, composed of two partially homologous subunits, alpha and beta, shares some of the properties of eucaryotic histones and is a major constituent of the bacterial nucleoid. We report here the construction of double mutants totally lacking both subunits of HU protein. These mutants exhibited poor growth and a perturbation of cell division, resulting in the formation of anucleate cells. In the absence of HU, phage Mu was unable to grow, to lysogenize,...

  4. [Doctor HUANG Shi-ping's acupuncture with golden needles].

    Science.gov (United States)

    Chen, Teng-Fei; Ma, Zeng-Bin; Xin, Si-Yuan; Zhu, Jiang

    2013-08-01

    Taking Doctor HUANG Shi-ping as the representative, the school of Huang's golden needle is based on Chinese martial art. Golden needles are adopted as main tool. Attaching great importance on the combination of acupuncture and moxibustioin, it is also characterized with penetrating needling with long needles. Through the development of three generations, it once outshone other schools in the field of acupuncture, and became famous all over the world. It made great contribution to the development of the course of acupuncture. However, with the development of the history, the form of acupuncture education as well as apparatus were all undergone an unified reform. Therefore, Doctor HUANG Shi-ping's acupuncture school be lost gradually.

  5. A SHiP to explore new routes

    CERN Multimedia

    Antonella Del Rosso

    2015-01-01

    SHiP (Search for Hidden Particles) is a proposed new facility for CERN's SPS accelerator. Its challenging goals include a direct search for hidden non-Standard-Model particles. Predicted by a large number of theoretical models, such particles could have slipped under the radar of the LHC experiments because of their specific features. In its first symposium held at CERN on 2 July, SHiP scientists showed the broader scientific community how they plan to unveil the hidden world.     The SHiP experiment is designed to be installed next to the SPS North Area.  Powerful accelerators like the LHC allow physicists to explore extensively the primordial phenomena at energy densities equivalent to those that existed just a few moments after the Big Bang. In this way, thanks to the high precision of the LHC experiments, scientists have been able to comprehensively confirm the predictions of the Standard Model (SM), including the existence of a Higgs boson. However, new...

  6. Shy Couplings, CAT(0) Spaces, and the Lion and Man

    CERN Document Server

    Bramson, Maury; Kendall, Wilfrid S

    2010-01-01

    Two random processes X and Y on a metric space are said to be epsilon-shy coupled if there is positive probability of them staying at least a positive distance epsilon apart from each other forever. Interest in the literature centres on nonexistence results subject to topological and geometric conditions; motivation arises from the desire to gain a better understanding of probabilistic coupling. Previous non-existence results for co-adapted shy coupling of reflected Brownian motion required convexity conditions; we remove these conditions by showing the non-existence of shy co-adapted couplings of reflecting Brownian motion in any bounded CAT(0) domain with boundary satisfying uniform exterior sphere and interior cone conditions, for example, simply-connected bounded planar domains with C2 boundary. The proof uses a Cameron-Martin-Girsanov argument, together with a continuity property of the Skorokhod transformation and properties of the intrinsic metric of the domain. To this end, a generalization of Gauss' ...

  7. A Working Day of Professor Hu Yamei

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    Professor Hu Yamei is a well-known pediatrician and one of the first group of academicians to the Institute of Medical Science attached to the Chinese Academy of Engineering. At the start of her career in the 1950s, she focused on nutritional anemia that afflicts children, developing treatment and methods of prevention. Later she researched etiology, pathology and physiology of pediatric toxic indigestion, helping to reduce the child death rate from 20 percent to 1 percent. Over the past 20 years, she has concentrated on pediatric acute lymphoblastic leukemia. As a result, the five-years living rate of the disease treated by Hu’s group has reached 74 percent, approaching the highest level in the world.

  8. Notas sobre el paisaje de Huánuco Pampa

    OpenAIRE

    Román Godines, Oscar; Ministerio de Cultura

    2013-01-01

    El complejo arqueológico Huánuco Pampa se encuentra ubicado en la provincia Dos de Mayo en el departamento de Huánuco y en la actualidad es quizás el principal vestigio de la cosmovisión y la planificación urbana inca.

  9. Rapid purification of HU protein from Halobacillus karajensis

    Directory of Open Access Journals (Sweden)

    Parinaz Ghadam

    2014-03-01

    Full Text Available The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to cruciform DNA and repair intermediates such as nick, gap, bulge, etc. To understand the features of HU binding to DNA and repair intermediates, a fast and easy HU protein purification method is required. Here we report a two-step purification procedure of HU from Halobacillus karajensis (the gram positive and moderately halophilic bacteria isolated from Karaj surface soil. The method of HU purification allows obtaining a pure non-tagged protein. Salting out and ion exchange chromatography were applied for purification, and the purified protein was identified by immunoblotting. Results showed that the molecular weight of the purified protein was approximately 11 kDa which is immunologically similar to the Bacillus subtilis HU protein (HBsu.

  10. $\\mu$-flux measurements for SHiP using NA61/SHINE

    CERN Document Server

    Dijkstra, H; Korzenev, A; Mermod, P

    2016-01-01

    A major concern for the design of the SHiP experiment is the lack of a precise knowledge of the muon flux. This is a proposal to measure the expected muon flux in the SHiP experiment by installing a replica of the SHiP target in a 400 GeV/c proton beam in front of the NA61/SHINE spectrometer. We propose to do a first measurement in june 2017.

  11. Sparks sädeleb Rabarocki öös. Ettevaatust! Eliidi rünnak. Hu? Hu da hell?

    Index Scriptorium Estoniae

    2007-01-01

    Ameerika ansamblist Sparks. Vaiko Eplik & Eliit heliplaatidest "1: Aastaajad", "2: Aastaajad" ja "3: Kosmoseodüsseia", esinemisest festivalil Rabarock. Hannaliisa Uusma, Leslie Da Bassi ja DJ Criticali projektist HU?, singlitest "Kosmos" ja "Absoluutselt" (Rabarockil esitusel materjal HU? debüütalbumilt "Film")

  12. Sparks sädeleb Rabarocki öös. Ettevaatust! Eliidi rünnak. Hu? Hu da hell?

    Index Scriptorium Estoniae

    2007-01-01

    Ameerika ansamblist Sparks. Vaiko Eplik & Eliit heliplaatidest "1: Aastaajad", "2: Aastaajad" ja "3: Kosmoseodüsseia", esinemisest festivalil Rabarock. Hannaliisa Uusma, Leslie Da Bassi ja DJ Criticali projektist HU?, singlitest "Kosmos" ja "Absoluutselt" (Rabarockil esitusel materjal HU? debüütalbumilt "Film")

  13. Testing the planetary models of HU Aquarii

    CERN Document Server

    Bours, M; Breedt, E; Copperwheat, C; Dhillon, V; Leckngam, A; Littlefair, S; Parsons, S; Prasit, A

    2014-01-01

    We present new eclipse observations of the polar (i.e. semi-detached magnetic white dwarf + M-dwarf binary) HU Aqr, and mid-egress times for each eclipse, which continue to be observed increasingly early. Recent eclipses occurred more than 70 seconds earlier than the prediction from the latest model that invoked a single circumbinary planet to explain the observed orbital period variations, thereby conclusively proving this model to be incorrect. Using ULTRACAM data, we show that mid-egress times determined for simultaneous data taken at different wavelengths agree with each other. The large variations in the observed eclipse times cannot be explained by planetary models containing up to three planets, because of poor fits to the data as well as orbital instability on short time scales. The peak-to-peak amplitude of the O-C diagram of almost 140 seconds is also too great to be caused by Applegate's mechanism, movement of the accretion spot on the surface of the white dwarf, or by asynchronous rotation of the ...

  14. First steps towards geometry optimization for Spectrometer Straw Tracker of SHiP detector

    CERN Document Server

    Solovev, Vladimir

    2017-01-01

    This report contains details of CERN Summer Student project which was performed for SHiP experiment (Search for Hidden Particles). The main aim of the project is optimization of Spectrometer Straw Tracker (SST) geometry implemented in FairSHiP simulation program.

  15. Dr.Shi Yanqing's Experience in Acupuncture Treatment of Paralysis Agitans

    Institute of Scientific and Technical Information of China (English)

    施孝文

    2004-01-01

    @@ Dr. Shi Yanqing (施延庆), a chief physician of traditional Chinese medicine, is an expert using warm-needling therapy to treat obstinate and miscellaneous diseases. In his long years of clinical practice, Dr. Shi has treated several tens of paralysis agitans cases, and obtained quite good therapeutic effects as is introduced in the following.

  16. Monitoring the Surface Heat Island (shi) Effects of Industrial Enterprises

    Science.gov (United States)

    Şekertekin, A.; Kutoglu, Ş. H.; Kaya, S.; Marangoz, A. M.

    2016-06-01

    The aim of this study is to present the effects of industrial enterprises on Land Surface Temperature (LST) and to retrieve Surface Heat Island (SHI) maps of these regions. SHI is one of the types of Urban Heat Island (UHI) and as the urban areas grow in a city, UHI effect becomes bigger. The city centre of Zonguldak was chosen as study area and Landsat 5 satellite data were used as materials. Zonguldak has important industrial enterprises like thermal power plants and iron and steel plant. ERDEMIR is the biggest iron and steel plant in Turkey and it is one of the biggest ones in Europe, as well. There are three operating thermal power plants in the region namely CATES, ZETES1 and ZETES2. In order to investigate these industrial regions, Landsat 5 satellite data were processed using mono-window algorithm to retrieve LST and they were acquired on 11.09.1987, 18.09.2007 and 29.09.2011, respectively. The obtained results revealed that from 1987 to 2011, spatial and temporal variability in LST in industrial enterprises became higher than the surroundings. Besides, the sizes of SHIs in 2011 are bigger than the ones in 1987. For the countries and governments, having industrial enterprises is crucial for the development and it is also important to present the community better conditions in life. Thus, decision makers should consider mitigating the effects of these regions on LST.

  17. MONITORING THE SURFACE HEAT ISLAND (SHI EFFECTS OF INDUSTRIAL ENTERPRISES

    Directory of Open Access Journals (Sweden)

    A. Şekertekin

    2016-06-01

    Full Text Available The aim of this study is to present the effects of industrial enterprises on Land Surface Temperature (LST and to retrieve Surface Heat Island (SHI maps of these regions. SHI is one of the types of Urban Heat Island (UHI and as the urban areas grow in a city, UHI effect becomes bigger. The city centre of Zonguldak was chosen as study area and Landsat 5 satellite data were used as materials. Zonguldak has important industrial enterprises like thermal power plants and iron and steel plant. ERDEMIR is the biggest iron and steel plant in Turkey and it is one of the biggest ones in Europe, as well. There are three operating thermal power plants in the region namely CATES, ZETES1 and ZETES2. In order to investigate these industrial regions, Landsat 5 satellite data were processed using mono-window algorithm to retrieve LST and they were acquired on 11.09.1987, 18.09.2007 and 29.09.2011, respectively. The obtained results revealed that from 1987 to 2011, spatial and temporal variability in LST in industrial enterprises became higher than the surroundings. Besides, the sizes of SHIs in 2011 are bigger than the ones in 1987. For the countries and governments, having industrial enterprises is crucial for the development and it is also important to present the community better conditions in life. Thus, decision makers should consider mitigating the effects of these regions on LST.

  18. CB2 cannabinoid receptor agonist enantiomers HU-433 and HU-308: An inverse relationship between binding affinity and biological potency.

    Science.gov (United States)

    Smoum, Reem; Baraghithy, Saja; Chourasia, Mukesh; Breuer, Aviva; Mussai, Naama; Attar-Namdar, Malka; Kogan, Natalya M; Raphael, Bitya; Bolognini, Daniele; Cascio, Maria G; Marini, Pietro; Pertwee, Roger G; Shurki, Avital; Mechoulam, Raphael; Bab, Itai

    2015-07-14

    Activation of the CB2 receptor is apparently an endogenous protective mechanism. Thus, it restrains inflammation and protects the skeleton against age-related bone loss. However, the endogenous cannabinoids, as well as Δ(9)-tetrahydrocannabinol, the main plant psychoactive constituent, activate both cannabinoid receptors, CB1 and CB2. HU-308 was among the first synthetic, selective CB2 agonists. HU-308 is antiosteoporotic and antiinflammatory. Here we show that the HU-308 enantiomer, designated HU-433, is 3-4 orders of magnitude more potent in osteoblast proliferation and osteoclast differentiation culture systems, as well as in mouse models, for the rescue of ovariectomy-induced bone loss and ear inflammation. HU-433 retains the HU-308 specificity for CB2, as shown by its failure to bind to the CB1 cannabinoid receptor, and has no activity in CB2-deficient cells and animals. Surprisingly, the CB2 binding affinity of HU-433 in terms of [(3)H]CP55,940 displacement and its effect on [(35)S]GTPγS accumulation is substantially lower compared with HU-308. A molecular-modeling analysis suggests that HU-433 and -308 have two different binding conformations within CB2, with one of them possibly responsible for the affinity difference, involving [(35)S]GTPγS and cAMP synthesis. Hence, different ligands may have different orientations relative to the same binding site. This situation questions the usefulness of universal radioligands for comparative binding studies. Moreover, orientation-targeted ligands have promising potential for the pharmacological activation of distinct processes.

  19. On the HU Aquarii planetary system hypothesis

    Science.gov (United States)

    Goździewski, Krzysztof; Nasiroglu, Ilham; Słowikowska, Aga; Beuermann, Klaus; Kanbach, Gottfried; Gauza, Bartosz; Maciejewski, Andrzej J.; Schwarz, Robert; Schwope, Axel D.; Hinse, Tobias C.; Haghighipour, Nader; Burwitz, Vadim; Słonina, Mariusz; Rau, Arne

    2012-09-01

    In this paper, we investigate the eclipse timing of the polar binary HU Aquarii that has been observed for almost two decades. Recently, Qian et al. attributed large (O-C) deviations between the eclipse ephemeris and observations to a compact system of two massive Jovian companions. We improve the Keplerian, kinematic model of the light travel time effect and re-analyse the whole currently available data set. We add almost 60 new, yet unpublished, mostly precision light curves obtained using the time high-resolution photopolarimeter Optical Timing Analyzer (OPTIMA), as well as photometric observations performed at the Monitoring Network of Telescopes/North, Physics Innovations Robotic Astronomical Telescope Explorer and Carlos Sánchez Telescope. We determine new mid-egress times with a mean uncertainty at the level of 1 s or better. We claim that because the observations that currently exist in the literature are non-homogeneous with respect to spectral windows (ultraviolet, X-ray, visual and polarimetric mode) and the reported mid-egress measurements errors, they may introduce systematics that affect orbital fits. Indeed, we find that the published data, when taken literally, cannot be explained by any unique solution. Many qualitatively different and best-fit two-planet configurations, including self-consistent, Newtonian N-body solutions may be able to explain the data. However, using high-resolution, precision OPTIMA light curves, we find that the (O-C) deviations are best explained by the presence of a single circumbinary companion orbiting at a distance of ˜4.5 au with a small eccentricity and having ˜7 Jupiter masses. This object could be the next circumbinary planet detected from the ground, similar to the announced companions around close binaries HW Vir, NN Ser, UZ For, DP Leo, FS Aur or SZ Her, and planets of this type around Kepler-16, Kepler-34 and Kepler-35.

  20. Development and validation of the shy bladder scale.

    Science.gov (United States)

    Deacon, Brett J; Lickel, James J; Abramowitz, Jonathan S; McGrath, Patrick B

    2012-01-01

    Paruresis, characterized by the difficulty or inability to urinate in a variety of social contexts, is a scientifically under-studied phenomenon. One reason for this state of affairs is the paucity of reliable and valid measures for assessing this problem. The present article attempted to address this limitation by investigating the psychometric properties and validity of a new measure of paruresis: the Shy Bladder Scale (SBS). In two undergraduate samples, the SBS demonstrated excellent internal consistency and a stable factor structure assessing difficulty urinating in public, impairment and distress, and paruresis-related fear of negative evaluation. Undergraduate students evidenced very low levels of paruresis-related concerns. In contrast, SBS scores were markedly elevated among individuals recruited from an online support network who appeared to meet diagnostic criteria for paruresis-specific social phobia. Our findings highlight the SBS's potential utility as a measure of paruresis in clinical and research contexts.

  1. Search for hidden particles with the SHiP experiment

    Energy Technology Data Exchange (ETDEWEB)

    Hagner, Caren; Bick, Daniel; Bieschke, Stefan; Ebert, Joachim; Schmidt-Parzefall, Walter [Universitaet Hamburg, Institut fuer Experimentalphysik, Luruper Chaussee 149, 22761 Hamburg (Germany)

    2016-07-01

    Many theories beyond the standard model predict long lived neutral (hidden) particles. There might be a whole Hidden Sector (HS) of weakly interacting particles, which cannot be detected in existing high energy experiments. The SHiP experiment (Search for Hidden Particles) requires a high intensity beam dump, which could be realized by a new facility at the CERN SPS accelerator. New superweakly interacting particles with masses below O(10) GeV could be produced in the beam dump and detected in a general purpose Hidden Sector (HS) detector. In addition there will be a dedicated tau neutrino subdetector. I present the major requirements and technical challenges for the HS detector and discuss how the HS can be accessed through several portals: neutrino portal, scalar portal, vector portal and many more.

  2. Infant shy temperament predicts preschoolers Theory of Mind.

    Science.gov (United States)

    Mink, Daniela; Henning, Anne; Aschersleben, Gisa

    2014-02-01

    The aim of this longitudinal study was to investigate the relation between infant temperament at 18 months and early Theory of Mind (ToM) abilities at 3 years of age. Temperament was assessed with the Early Childhood Behavior Questionnaire (ECBQ) and ToM by assessing children's understanding of divergent desires and beliefs, and of knowledge access. Our results are in line with a social-emotional reactivity perspective postulating more sophisticated ToM abilities for children with less reactive more observant temperament. Children with shy temperament at 18 months and at 3 years were better in reasoning about others' mental states at age 3. Language, siblings and parental education had no effect on ToM. Findings indicate that temperament is related to ToM earlier in development than previously found, and that this relation is thus not unique to false-belief understanding.

  3. The computer-assisted interview In My Shoes can benefit shy preschool children's communication

    Science.gov (United States)

    Salari, Raziye; Eriksson, Maria; Sarkadi, Anna

    2017-01-01

    Interviewing children is a cognitively, socially, and emotionally challenging situation, especially for young and shy children. Thus, finding methods that aid rapport and increase these children’s communication is important. The present study investigated whether children’s verbal and non-verbal communicative behavior developed differently during the rapport phase, depending on whether children were situationally shy or not, and whether the interview was conducted using the computer-assisted interview In My Shoes (IMS) or a Standard verbal interview. The sample consisted of 60 children aged 4 to 5-years-old. The results showed that for the shy children in the IMS group their talkativeness increased and their answer latency decreased including the amount of encouragement the child needed to talk, while no changes were observed for the shy children in the Standard verbal interview group. There were no significant differences in the non-verbal behavior for the shy children regardless of the interview method used. For the non-shy children, overall, the interview method did not affect either the verbal or the non-verbal outcomes. Our findings indicate that IMS can be a useful tool during the rapport-building phase with shy children as it helps these children to improve their verbal communication. PMID:28813534

  4. Shy birds play it safe: personality in captivity predicts risk responsiveness during reproduction in the wild.

    Science.gov (United States)

    Cole, Ella F; Quinn, John L

    2014-05-01

    Despite a growing body of evidence linking personality to life-history variation and fitness, the behavioural mechanisms underlying these relationships remain poorly understood. One mechanism thought to play a key role is how individuals respond to risk. Relatively reactive and proactive (or shy and bold) personality types are expected to differ in how they manage the inherent trade-off between productivity and survival, with bold individuals being more risk-prone with lower survival probability, and shy individuals adopting a more risk-averse strategy. In the great tit (Parus major), the shy-bold personality axis has been well characterized in captivity and linked to fitness. Here, we tested whether 'exploration behaviour', a captive assay of the shy-bold axis, can predict risk responsiveness during reproduction in wild great tits. Relatively slow-exploring (shy) females took longer than fast-exploring (bold) birds to resume incubation after a novel object, representing an unknown threat, was attached to their nest-box, with some shy individuals not returning within the 40 min trial period. Risk responsiveness was consistent within individuals over days. These findings provide rare, field-based experimental evidence that shy individuals prioritize survival over reproductive investment, supporting the hypothesis that personality reflects life-history variation through links with risk responsiveness.

  5. An Analysis of HU Shi’ s Career Selection in the US:from the Perspective of Social and Cultural History%胡适留美择业的社会文化史解析

    Institute of Scientific and Technical Information of China (English)

    徐希军

    2016-01-01

    HU Shi firstly selected agriculture as his major when he studied in the United States. It was determined by two fac-tors. On the one hand,“Science is useful” was a fashion of the times, and on the other, money saved from the tuition-free major could be a great help to his family. Chinese overseas students’ career selection reflected the profound influence on education from the change of Chinese political ecology. Since he had a preference for literature and history and thanks to the credit system, HU Shi later on abandoned agriculture for literature, which was a turning point of his life.%胡适留学美国初选农科,其决定性因素是“科学”有用的从众心理的作用和家庭境遇的考量。留学生的择业价值取向也折射出中国政治生态变化对教育的深刻影响。胡适后来弃农从文,缘于偏好文史的性情,成于学分制的支持,是其一生志业的转折点。

  6. The Sogdian Trade Caravan Traveled Between Liu Hu Zhou and LuoYang in Tang Dynasty%唐代六胡州、洛阳间的粟特商队

    Institute of Scientific and Technical Information of China (English)

    岳东

    2015-01-01

    The Liu Hu Zhou trade caravan is still at work on long distance trade in Tang Dynasty.Shi Zhou attracts the caravan to live there for its traffic hub position,economic hinterland superiority condition.The trade route starting from Liu Hu Zhou, passing Shi Zhou and finally arriving at Luo Yang is in a good condition to travel. Wu Ding River Valley, Fen River Valley, and the influx of husbandry, towns, and traffic have provided great convenience for long distant trade. Further, the image of caravan Zoroastrian gods comes from here.%唐代六胡州仍保持着经商的传统,从事着远程长途贸易。石州因着地理交通的便利,经济腹地的支撑,吸引了大量六胡州粟特商队前来定居,形成聚落。从六胡州出发,经石州水陆转运,通往洛阳的商路十分畅通,无定河谷、汾河谷道中,农牧业、城镇、交通的汇集,为远程商贸的展开提供了便利,骆驼祆神形象就诞生在这条路上。

  7. Revolution, Modernity and (Trans)National Shi`i Islam: Rethinking Religious Conversion in Senegal.

    Science.gov (United States)

    Leichtman, Mara A

    2009-07-01

    The establishment of a Shi`i Islamic network in Senegal is one alternative to following the country's dominant Sufi orders. I examine Senegalese conversion narratives and the central role played by the Iranian Revolution, contextualizing life stories (trans)nationally in Senegal's political economy and global networks with Iran and Lebanon. Converts localize foreign religious ideologies into a 'national' Islam through the discourse that Shi`i education can bring peace and economic development to Senegal. Senegalese Shi`a perceive that proselytizing, media technologies, and Muslim networking can lead to social, cultural and perhaps even political change through translating the Iranian Revolution into a non-violent reform movement.

  8. R-Parity Violation and Light Neutralinos at SHiP and the LHC

    CERN Document Server

    de Vries, Jordy; Schmeier, Daniel

    2015-01-01

    We study the sensitivity of the proposed SHiP experiment to the LQD operator in R-parity violating supersymmetric theories. We focus on single neutralino production via rare meson decays and the observation of downstream neutralino decays into charged mesons inside the SHiP decay chamber. We provide a generic list of effective operators and decay width formulae for any LQD coupling and show the resulting expected SHiP sensitivity for a widespread list of benchmark scenarios via numerical simulations. We compare this sensitivity to expected limits from testing the same decay topology at the LHC with ATLAS.

  9. Disease progresses more quickly in introverts. Shy, inhibited personalities may fare worse.

    Science.gov (United States)

    2004-03-01

    Los Angeles researchers recently have discovered clinical evidence that HIV-positive people with shy and introverted personalities tend to have a faster disease progression and less optimal outcomes under antiretroviral treatment than do people with extroverted personalities.

  10. 差比序列视角下“不是A但是/只是/而是B”之比较%Comparison of “Bu Shi(不是) A Dan Shi(但是)/Zhi Shi(只是)/Er Shi(而是) B”from the View of Semantic Gap Sequence

    Institute of Scientific and Technical Information of China (English)

    王振

    2013-01-01

    语言中的某些词或词组可以形成一个差比序列。将不同差比序列项分别嵌入“不是A但是/只是/而是B”框架,对其嵌入条件、语义内涵和否定类别分别加以比较,可从新的视角发现这三个框架深层次的区别与联系。%Some words and phrases can form a semantic gap sequence. By inserting different items of the sequence into the structures of “bu shi A dan shi/ zhi shi/ er shi B”, this paper compares their inserting conditions, semantic connotations and negative catego-ries, finding out some underlying similarities and differences of the three structures from a new angle of view.

  11. CAS President Offers Condolences over the Death of Mr. Shiing-shen Chern

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ On the demise of Mr.Shiing-shen Chern,CAS President Lu Yongxiang sent his letter of condolences on Dec. 5 to Nankai University, where the noted mathematician had served as director of its mathematics center.

  12. Muon-flux measurements for SHiP at H4

    CERN Document Server

    van Herwijnen, E

    2017-01-01

    A major concern for the design of the SHiP experiment is the lack of a precise knowledge of the muon flux. This is a proposal to measure the expected muon flux in the SHiP experiment by installing a replica of the SHiP target in a 400 GeV/c proton beam at H4. We intend building a spectrometer using the drift tube prototypes that were constructed for OPERA. A muon tagger will be built using RPCs, which will also serve as a module-0 for SHiP. We propose to do this measurement in early 2018. Accumulating $\\sim 10^{11}$ 400 GeV/c POT will enable us to make a more realistic design of the muon shield. With some modifications, this setup can also be used to measure the charm cross section (including the cascade production). We intend to test this setup after the measurement of the muon flux.

  13. Sensitivity of the SHiP experiment to a light scalar particle mixing with the Higgs

    CERN Document Server

    Lanfranchi, Gaia

    2017-01-01

    This conceptual study shows the ultimate sensitivity of the SHiP experiment for the search of a light scalar particle mixing with the Higgs for a dataset corresponding to 5-years of SHiP operation at a nominal intensity of 4 1013 protons on target per second. The sensitivity as a function of the length of the vessel and of its distance from the target as well as a function of the background contamination is also studied.

  14. Hu Shi's Views on "Ultra-utilitarianism" Quality Education%胡适"超功利主义"素质教育观

    Institute of Scientific and Technical Information of China (English)

    艾亚玮; 刘爱华

    2010-01-01

    对胡适教育思想的研究,成果比较丰硕,但鲜有文章从素质教育角度加以探析.他的"超功利主义"素质教育观,主要体现在注意学科、学说的自由协调发展,重视生活教育、工具性学科教育,倡导实验主义治学方法.值得我们借鉴!

  15. Different Influences on Tacrolimus Pharmacokinetics by Coadministrations of Zhi Ke and Zhi Shi in Rats

    Directory of Open Access Journals (Sweden)

    Shiuan-Pey Lin

    2011-01-01

    Full Text Available Tacrolimus, an immunosuppressant with narrow therapeutic window, has been used widely in transplant patients. Grapefruit juice and pomelo have been reported to increase the blood levels of tacrolimus. Zhi Ke and Zhi Shi, the ripe peels and unripe fruits of Citrus aurantium which is chemotaxonomically related to grapefruit and pomelo, are in wide use in clinical Chinese medicine. To investigate the possible interaction of these two Citrus herbs with tacrolimus, male Sprague-Dawley rats were orally given tacrolimus (1.5 mg/kg with and without Zhi Ke and Zhi Shi decoctions in a cross-over design. Blood samples were withdrawn via cardiopuncture at specific time and quantitated by a microparticle enzyme immunoassay. In addition, to explore the mechanism of interaction, LS 180 cell line was used for the transport study of rhodamine 123, a typical substrate of P-glycoprotein (P-gp. The results showed that Zhi Shi significantly decreased the Cmax⁡ and AUC0-t of tacrolimus by 72.4% and 72.0%, respectively, whereas Zhi Ke did not affect tacrolimus pharmacokinetics. LS 180 cell line study indicated that Zhi Shi increased the efflux activity of P-gp, enabling us to explain the decreased oral bioavailability of tacrolimus caused by Zhi Shi. Hence, we suggest that Zhi Shi be contraindicated for transplant patients treated with tacrolimus to reduce the risk of allograft rejection.

  16. Treatment of β-Thalassemia With Hydroxyurea (HU)——Effects of HU on Globin Gene Expression

    Institute of Scientific and Technical Information of China (English)

    黄淑帧; 任兆瑞; 陈美珏; 许洪平; 曾溢滔; G.P.Rodgers; 曾凡一; A.N.Schechter

    1994-01-01

    A newly developed method of RT-PCR/competitive PCR for measuring the relative and ab-solute content of globin mRNAs as well as micro-globin chain biosynthetic assay have been used to study thealterations of globin gene expressions in the patients with β-thalassemia pre-and post-hydroxyurea(HU)treatment.It was found for the first time that HU had the effect of enhancing β-globin gene expression insome patients.Two cases with β-thalassemia who were subjected to HU treatment for over two years showeda marked increase in β-globin mRNA level and β-globin chain synthesis,resulting in more effective erythro-poiesis and the alleviation of clinical symptoms.

  17. The SHiP experiment at CERN SPS

    CERN Document Server

    Di Crescenzoon, A

    2016-01-01

    SHiP is a new general purpose fixed target facility, whose Technical Proposal has been recently submitted to the CERN SPS Committee. In its initial phase, the 400 GeV proton beam extracted from the SPS will be dumped on a heavy target with the aim of integrating 2 × 1020 pot in 5 years. A dedicated detector located downstream of the target, based on a long vacuum tank followed by a spectrometer and particle identification detectors, will allow probing a variety of models with light long-lived exotic particles and masses below a few GeV/c2. The beam dump is also an ideal source of tau neutrinos, the less known particle in the Standard Model. Another dedicated detector, based on the Emulsion Cloud Chamber technology already used in the OPERA experiment, will allow to perform for the first time measurements of the tau neutrino deep inelastic scattering cross section. Tau neutrinos will be distinguished from tau anti-neutrinos, thus providing the first observation of the tau anti-neutrino.

  18. Neutrino physics with the SHiP experiment

    CERN Document Server

    AUTHOR|(SzGeCERN)759942

    2015-01-01

    Despite the Standard Model (SM) has been strongly confirmed by the Higgs discovery, several experimental facts are still not explained. The SHiP experiment (Search for Hidden Particles), a beam dump experiment at CERN, aims at the observation of long lived particles very weakly coupled with ordinary matter. These particles of the GeV mass scale, foreseen in many extensions of the SM, might come from the decay of charmed hadrons produced in the collision of a 400 GeV proton beam on a target. High rates of all the three active neutrinos are also expected. For the first time the properties and the cross section of the ντ will be studied thanks to a detector based on nuclear emulsions, with the micrometric resolution needed to identify the tau lepton produced in neutrino interactions. Measuring the charge of the tau daughters, will enable the first observation of the ν ̄τ and the study of its cross section.

  19. HU-444, a Novel, Potent Anti-Inflammatory, Nonpsychotropic Cannabinoid.

    Science.gov (United States)

    Haj, Christeene G; Sumariwalla, Percy F; Hanuš, Lumír; Kogan, Natalya M; Yektin, Zhana; Mechoulam, Raphael; Feldmann, Mark; Gallily, Ruth

    2015-10-01

    Cannabidiol (CBD) is a component of cannabis, which does not cause the typical marijuana-type effects, but has a high potential for use in several therapeutic areas. In contrast to Δ(9)-tetrahydrocannabinol (Δ(9)-THC), it binds very weakly to the CB1 and CB2 cannabinoid receptors. It has potent activity in both in vitro and in vivo anti-inflammatory assays. Thus, it lowers the formation of tumor necrosis factor (TNF)-α, a proinflammatory cytokine, and was found to be an oral antiarthritic therapeutic in murine collagen-induced arthritis in vivo. However, in acidic media, it can cyclize to the psychoactive Δ(9)-THC. We report the synthesis of a novel CBD derivative, HU-444, which cannot be converted by acid cyclization into a Δ(9)-THC-like compound. In vitro HU-444 had anti-inflammatory activity (decrease of reactive oxygen intermediates and inhibition of TNF-α production by macrophages); in vivo it led to suppression of production of TNF-α and amelioration of liver damage as well as lowering of mouse collagen-induced arthritis. HU-444 did not cause Δ(9)-THC-like effects in mice. We believe that HU-444 represents a potential novel drug for rheumatoid arthritis and other inflammatory diseases.

  20. HuR in pheochromocytomas and paragangliomas - overexpression in verified malignant tumors.

    Science.gov (United States)

    Leijon, Helena; Salmenkivi, Kaisa; Heiskanen, Ilkka; Hagström, Jaana; Louhimo, Johanna; Heikkilä, Päivi; Ristimäki, Ari; Paavonen, Timo; Metso, Saara; Mäenpää, Hanna; Haglund, Caj; Arola, Johanna

    2016-09-01

    Pheochromocytomas and paragangliomas are rare, neural crest-originating, neuroendocrine tumors. HuR is an mRNA-binding protein of the ELAV/Hu-protein family, which participates in posttranscriptional regulation of many cancer-associated genes. HuR expression has been connected with aggressive behavior of several malignancies. Cyclooxygenase-2 (COX-2) is also expressed in several malignant tumors, and its expression is regulated by HuR. Tissue microarray of 153 primary pheochromocytomas and paragangliomas was investigated for the expression of HuR and COX-2 proteins by immunohistochemistry using two different HuR antibodies (HuR19F12 and HuR3A). In these tumors, the expression of both intranuclear and cytoplasmic HuR was detectable. Increased cytoplasmic HuR expression was significantly associated with metastatic tumors. Increased COX-2 and MIB-1 expression also was associated with metastatic potential, and moreover, HuR and COX-2 expression correlated with each other. Our data suggest that increased expression of HuR protein is associated with metastatic potential of paragangliomas and pheochromocytomas, and COX-2 seems to be a target of HuR.

  1. Structure-based analysis of HU-DNA binding.

    Science.gov (United States)

    Swinger, Kerren K; Rice, Phoebe A

    2007-01-26

    HU and IHF are prokaryotic proteins that induce very large bends in DNA. They are present in high concentrations in the bacterial nucleoid and aid in chromosomal compaction. They also function as regulatory cofactors in many processes, such as site-specific recombination and the initiation of replication and transcription. HU and IHF have become paradigms for understanding DNA bending and indirect readout of sequence. While IHF shows significant sequence specificity, HU binds preferentially to certain damaged or distorted DNAs. However, none of the structurally diverse HU substrates previously studied in vitro is identical with the distorted substrates in the recently published Anabaena HU(AHU)-DNA cocrystal structures. Here, we report binding affinities for AHU and the DNA in the cocrystal structures. The binding free energies for formation of these AHU-DNA complexes range from approximately 10-14.5 kcal/mol, representing K(d) values in the nanomolar to low picomolar range, and a maximum stabilization of at least approximately 6.3 kcal/mol relative to complexes with undistorted, non-specific DNA. We investigated IHF binding and found that appropriate structural distortions can greatly enhance its affinity. On the basis of the coupling of structural and relevant binding data, we estimate the amount of conformational strain in an IHF-mediated DNA kink that is relieved by a nick (at least 0.76 kcal/mol) and pinpoint the location of the strain. We show that AHU has a sequence preference for an A+T-rich region in the center of its DNA-binding site, correlating with an unusually narrow minor groove. This is similar to sequence preferences shown by the eukaryotic nucleosome.

  2. Structure-based Analysis to Hu-DNA Binding

    Energy Technology Data Exchange (ETDEWEB)

    Swinger,K.; Rice, P.

    2007-01-01

    HU and IHF are prokaryotic proteins that induce very large bends in DNA. They are present in high concentrations in the bacterial nucleoid and aid in chromosomal compaction. They also function as regulatory cofactors in many processes, such as site-specific recombination and the initiation of replication and transcription. HU and IHF have become paradigms for understanding DNA bending and indirect readout of sequence. While IHF shows significant sequence specificity, HU binds preferentially to certain damaged or distorted DNAs. However, none of the structurally diverse HU substrates previously studied in vitro is identical with the distorted substrates in the recently published Anabaena HU(AHU)-DNA cocrystal structures. Here, we report binding affinities for AHU and the DNA in the cocrystal structures. The binding free energies for formation of these AHU-DNA complexes range from 10-14.5 kcal/mol, representing K{sub d} values in the nanomolar to low picomolar range, and a maximum stabilization of at least 6.3 kcal/mol relative to complexes with undistorted, non-specific DNA. We investigated IHF binding and found that appropriate structural distortions can greatly enhance its affinity. On the basis of the coupling of structural and relevant binding data, we estimate the amount of conformational strain in an IHF-mediated DNA kink that is relieved by a nick (at least 0.76 kcal/mol) and pinpoint the location of the strain. We show that AHU has a sequence preference for an A+T-rich region in the center of its DNA-binding site, correlating with an unusually narrow minor groove. This is similar to sequence preferences shown by the eukaryotic nucleosome.

  3. Shi Changxu--a great teacher and mentor for materials scientists%Shi Changxu--a great teacher and mentor for materials scientists

    Institute of Scientific and Technical Information of China (English)

    Gaoqing Max Lu

    2011-01-01

    I have met Professor Shi Changxu only a few times at meetings in China. One encounter that I can never forget is a meeting held in Beijing late December 2002. I was invited to participate in the inaugural meeting of the Centre for Interracial Materials of the Institute of Metal Research,

  4. HU-GFP and DAPI co-localize on the Escherichia coli nucleoid.

    Science.gov (United States)

    Wery, M; Woldringh, C L; Rouviere-Yaniv, J

    2001-02-01

    The heterodimeric HU protein, one of the most abundant DNA binding proteins, plays a pleiotropic role in bacteria. Among others, HU was shown to contribute to the maintenance of DNA superhelical density in Escherichia coli. By its properties HU shares some traits with histones and HMG proteins. More recently, its specific binding to DNA recombination and repair intermediates suggests that HU should be considered as a DNA damage sensor. For all these reasons, it will be of interest to follow the localization of HU within the living bacterial cells. To this end, we constructed HU-GFP fusion proteins and compared by microscopy the GFP green fluorescence with images of the nucleoid after DAPI staining. We show that DAPI and HU-GFP colocalize on the E. coli nucleoid. HU, therefore, can be considered as a natural tracer of DNA in the living bacterial cell.

  5. Targeting Mycobacterium tuberculosis nucleoid-associated protein HU with structure-based inhibitors

    Science.gov (United States)

    Bhowmick, Tuhin; Ghosh, Soumitra; Dixit, Karuna; Ganesan, Varsha; Ramagopal, Udupi A.; Dey, Debayan; Sarma, Siddhartha P.; Ramakumar, Suryanarayanarao; Nagaraja, Valakunja

    2014-06-01

    The nucleoid-associated protein HU plays an important role in maintenance of chromosomal architecture and in global regulation of DNA transactions in bacteria. Although HU is essential for growth in Mycobacterium tuberculosis (Mtb), there have been no reported attempts to perturb HU function with small molecules. Here we report the crystal structure of the N-terminal domain of HU from Mtb. We identify a core region within the HU-DNA interface that can be targeted using stilbene derivatives. These small molecules specifically inhibit HU-DNA binding, disrupt nucleoid architecture and reduce Mtb growth. The stilbene inhibitors induce gene expression changes in Mtb that resemble those induced by HU deficiency. Our results indicate that HU is a potential target for the development of therapies against tuberculosis.

  6. Genesis Research与Morphosys扩展HuCAL抗体协议

    Institute of Scientific and Technical Information of China (English)

    黄晓燕(摘)

    2008-01-01

    Genesis Research与Morphosys扩展协议后,靶向FGFR5蛋白的抗体将继续作为其研究的焦点。Genesis将利用Morphosys分公司Serotec开发的人组织抗体库(HuCAL)继续开发成纤维细胞生长因子受体5(FGFR5)靶点。

  7. The Chinese copula SHI and Its Origin: A Congnitive-based Approach

    Directory of Open Access Journals (Sweden)

    Jung-hsing Chang

    2006-06-01

    Full Text Available This paper discusses the proposals of Li and Thompson (1977, Yen (1986, and Feng (1993 in relation to the development of the Chinese copula and argues that Li and Thompson’s suggestion of a topic mechanism, Yen’s analogical change, and Feng’s phonological pause are unsatisfactory in explaining the development of the copula. It is suggested that Katz’s (1996 cognitive concept of existence in time and space between pronouns and copulas is what relates the demonstrative pronoun shi to a copula, while the verbal form shi occurring in the same syntactic context as the demonstrative pronoun shi is what triggers this demonstrative pronoun to turn into a copula.

  8. [Contribution of Professor SHI Xue-min's academic thoughts to treatment of stroke].

    Science.gov (United States)

    Liu, Jian; Fan, Xiao-Nong; Wang, Shu

    2014-01-01

    Based on the thought of Zhishen (a kind of mind regulation), Professor SHI Xue-min, academician of the China Academy of Engineering, found the Xingnao Kaiqiao (to refresh the mind and to cause resuscitation) acupuncture method, which still plays an important role in the acupuncture treatment of wind stroke nowadays. Meanwhile, great importance is attached to the comprehensive treatment of wind stroke. Danqi Piantan capsule (see text) is developed and "wind stroke unit" is set up. In recent years, Professor SHI shifts the center of research to the treatment of hypertension, the risk factor of wind stroke. Taking Renying (ST 9) as the major acupoint, acupuncture with standard measurement and manipulations is established. And good clinical effect has been obtained as well. Therefore, this article focuses on the introduction of Professor SHI Xue-min's contribution to wind stroke treatment.

  9. Personal autonomy in health settings and Shi'i Islamic Jurisprudence: a literature review.

    Science.gov (United States)

    Naji, Zohrehsadat; Zamani, Zari; Koutlaki, Sofia A; Salamati, Payman

    2016-10-03

    Respect for personal autonomy in decision-making is one of the four ethical principles in medical circumstances. This paper aims to present evidence that can be considered good exemplars in the clarification of the ethical viewpoints of the western and Shi'i Islamic perspectives on this issue. The method followed was originally a search in international indexing services in April 2016. Our findings point towards various controversies on individuals' autonomy lead to different decision making outcomes by health workers in both different traditions. We concluded that although Shi'i Islamic jurisprudence does not seem to allow for personal autonomy in the sense it is understood in a western context, evidence indicates that Shi'i Islamic jurisprudence respects personal autonomy.

  10. Enhancing Contextualized Curriculum: Integrated Identity in Young Shi'i Muslim Arabic-Canadian Students' Social Worlds

    Science.gov (United States)

    Al-Fartousi, May

    2016-01-01

    This research explored 10 young female Shi'i Muslim Arabic-Canadian students' experiences associated with wearing the Hijab (headscarf) within their home, community, and predominantly White Canadian public elementary school environments. The in-depth case study sought to address the dearth of information about Shi'is' experiences in schools…

  11. On Contributions of Hu Shi’s Reading Lvshi Chunqiu%胡适《读吕氏春秋》的贡献

    Institute of Scientific and Technical Information of China (English)

    王启才

    2015-01-01

    Reading Lvshi Chunqiu,collected in Collections ofHu Shi Essays III (vol.3 ),is a research paper on Lvshi Chunqiu written by Hu Shi in 1930.Shifting from the traditional emphasis on textual interpretation,the paper adopted a cont-rastive perspective,focusing on human desire and nature,and proposed quite a few valuable points of view.Based on histori-cal evidence,the paper took a broad scope of vision in writing,its writing style and manner were easy to accept,and its meth-od and conclusion were fresh and new.The paper preliminarily changed the roadmap of later research on Lvshi Chunqiu and became the pioneering work in the modern history of Lvshi Chunqiu research.%写于1930年,后收入《胡适文存三集》卷三的《读吕氏春秋》,是胡适的一篇研究《吕氏春秋》的论文。该文打破了有汉以来研究《吕氏春秋》只重视文本解释的局限,采用了中西比较的思路与方法,从人欲、人性的思想高度审视把握《吕氏春秋》,提出了不少现在看来仍不失为有价值的观点与问题。文章视野开阔,论从史出,文风明白晓畅,易于接受,方法与结论都令人耳目一新,初步改变了以后《吕氏春秋》研究的路向,在近代《吕氏春秋》研究史上具有开拓之功。

  12. HuCOP1 contributes to the regulation of DNA repair in keratinocytes.

    Science.gov (United States)

    Fazekas, B; Carty, M P; Németh, I; Kemény, L; Széll, M; Ádám, É

    2017-03-01

    We have previously demonstrated that the E3 ligase Human Constitutive Photomorphogenic Protein (huCOP1) is expressed in human keratinocytes and negatively regulates p53. The MutS homolog 2 (MSH2) protein plays a central role in DNA MMR mechanism and is implicated in the cellular response to anticancer agents, such as cisplatin. Our aim was to clarify whether huCOP1 plays a role in DNA MMR by affecting MSH2 protein level in human keratinocytes. To define the role of huCOP1 in DNA mismatch repair, we determined whether huCOP1 affects MSH2 abundance. MSH2 protein level was detected by immunocytochemical staining using a keratinocyte cell line in which the expression level of huCOP1 was stably decreased (siCOP1). To investigate whether huCOP1 silencing influences cisplatin-induced cell death, control and siCOP1 keratinocyte cells were treated with increasing concentrations of cisplatin and cell viability was recorded after 48 and 96 h. Stable silencing of huCOP1 in human keratinocytes resulted in a reduced level of MSH2 protein. huCOP1 silencing also sensitized keratinocytes to the interstrand crosslinking inducer cisplatin. Our results indicate that decreased huCOP1 correlates with lower MSH2 levels. These protein level changes lead to increased sensitivity toward cisplatin treatment, implicating that huCOP1 plays a positive role in maintaining genome integrity in human keratinocytes.

  13. Low level anti-Hu reactivity: a risk marker for small cell lung cancer?

    Science.gov (United States)

    Tsou, Jeffrey A.; Kazarian, Meleeneh; Patel, Ankur; Galler, Janice S.; Laird-Offringa, Ite A.; Carpenter, Catherine L.; London, Stephanie J.

    2010-01-01

    Background Previous experimental and laboratory studies have implicated antibodies against Hu proteins (anti-Hu) as a potential marker for small cell lung cancer (SCLC); there are no estimates of the association between anti-Hu and SCLC using a population-based design. Methods We used stored plasma specimens to evaluate anti-Hu reactivity in relationship to small cell lung cancer in a population-based case-control study. Using Western Blot analysis, we measured anti-Hu reactivity against recombinant Hu family member, HuD, in plasma samples from forty-one (41) SCLC cases and seventy-nine (79) controls individually matched for age, race, sex, and smoking status (never, past, current). We analyzed the association between anti-Hu reactivity and SCLC using conditional logistic regression. Results Anti-Hu reactivity was associated with SCLC, both before and after adjustment for amount of smoking. We observed a smoking-adjusted odds ratio of 3.2 (95 % confidence interval from 0.98 to 13.4) comparing subjects above 1800 units (the lower limit of the second tertile of the distribution among antibody positive controls) to subjects with lower reactivity. We also found suggestive evidence in follow-up of our cases that anti-Hu above 1800 units was related to longer-term survival from SCLC. The present research is the first report of anti-Hu reactivity and SCLC in a population-based study. Conclusions Given the suggestive evidence in this study, prospective analyses to examine whether anti-Hu reactivity might predict risk of developing SCLC, or whether anti-Hu reactivity could serve as an early marker for SCLC, may be warranted. PMID:19070439

  14. A Cognitive-Behavioral Approach to Social Skills Training with Shy Persons.

    Science.gov (United States)

    Haynes-Clements, Lynda A.; Avery, Arthur W.

    1984-01-01

    Developed, implemented, and evaluated a social skills training program for shy persons (N=12). Results indicated that subjects in the experimental group, relative to the control group, significantly decreased their level of social anxiety, decreased their negative self-statements, and increased their perceived ability to participate actively in…

  15. Canadian Early-Childhood Educators' Perceptions of Children's Gendered Shy, Aggressive, and Prosocial Behaviors

    Science.gov (United States)

    Woods, Heather; Bosacki, Sandra; Coplan, Robert J.

    2016-01-01

    Early childhood educators' (ECE) perceptions of gender roles may contribute to the development of children's own gender-role identities. This qualitative study examined 40 Canadian female ECEs' perceptions of gender and children's shy, aggressive, and prosocial behaviors. Content analysis of extensive interviews revealed three themes: (1) shyness…

  16. Canadian Early-Childhood Educators' Perceptions of Children's Gendered Shy, Aggressive, and Prosocial Behaviors

    Science.gov (United States)

    Woods, Heather; Bosacki, Sandra; Coplan, Robert J.

    2016-01-01

    Early childhood educators' (ECE) perceptions of gender roles may contribute to the development of children's own gender-role identities. This qualitative study examined 40 Canadian female ECEs' perceptions of gender and children's shy, aggressive, and prosocial behaviors. Content analysis of extensive interviews revealed three themes: (1) shyness…

  17. Canadian Elementary School Teachers' Perceptions of Gender Differences in Shy Girls and Boys in the Classroom

    Science.gov (United States)

    Akseer, Tabasum; Bosacki, Sandra L.; Rose-Krasnor, Linda; Coplan, Robert J.

    2014-01-01

    Shyness may play a significant role in children's socio-emotional and cognitive competence in the classroom. This qualitative study investigated elementary school teachers' perceptions of the concept of shyness in their students. In particular, we explored teachers' perceptions regarding the role that gender plays in shy children's behaviours, and…

  18. Preservice Teachers' Knowledge of Effective Classroom Management Strategies: Shy or Withdrawn Students.

    Science.gov (United States)

    Lacina-Gifford, Lorna J.; Kher, Neelam; Besant, Kyesha

    This study identified preservice teachers' knowledge about effective and ineffective classroom management strategies. A group of 108 preservice teachers at a southern rural public university generated classroom management strategies in response to hypothetical vignettes depicting shy and withdrawn student behavior. Researchers coded the extended…

  19. Treatment of Chronic Bronchitis with Modified Ma Xing Shi Gan Tang and Er Chen Tang

    Institute of Scientific and Technical Information of China (English)

    朱颖; 刘晓东

    2004-01-01

    @@ Chronic bronchitis is a disease commonly seen in the clinic. The authors have treated 78 cases of the disease with modified Ma Xing Shi Gan Tang (麻杏石甘汤 Decoction of Ephedra, Armeniacae Amarum, Glycyrrhizae and Gypsum Fibrosum) plus Er Chen Tang (二陈汤 Two Old Drugs Decoction). The results were satisfactory and reported as follows.

  20. The quantitative genetic architecture of the bold-shy continuum in zebrafish, Danio rerio.

    Directory of Open Access Journals (Sweden)

    Mary E Oswald

    Full Text Available In studies of consistent individual differences (personality along the bold-shy continuum, a pattern of behavioral correlations frequently emerges: individuals towards the bold end of the continuum are more likely to utilize risky habitat, approach potential predators, and feed under risky conditions. Here, we address the hypothesis that observed phenotypic correlations among component behaviors of the bold-shy continuum are a result of underlying genetic correlations (quantitative genetic architecture. We used a replicated three-generation pedigree of zebrafish (Danio rerio to study three putative components of the bold-shy continuum: horizontal position, swim level, and feeding latency. We detected significant narrow-sense heritabilities as well as significant genetic and phenotypic correlations among all three behaviors, such that fish selected for swimming at the front of the tank swam closer to the observer, swam higher in the water column, and fed more quickly than fish selected for swimming at the back of the tank. Further, the lines varied in their initial open field behavior (swim level and activity level. The quantitative genetic architecture of the bold-shy continuum indicates that the multivariate behavioral phenotype characteristic of a "bold" personality type may be a result of correlated evolution via underlying genetic correlations.

  1. Faith or Reason? The Shi'ite Tradition of Political Ideas in the Light of the Theological Debate

    Directory of Open Access Journals (Sweden)

    Mohammad Fazlhashemi

    2016-02-01

    Full Text Available The 11th February, 2010 marked the commemoration of the thirty-first anniversary of the Islamic revolution in Iran. In 1979, the Shi'ite Islamists assumed power there. Since then, Shi'a politics has played a dominant role in the development of both Iran and several other countries in the Middle East. This has included the restructuring of the political map in Iraq since the fall of the dictator Saddam Hussein, which has meant that Shi'ite political parties and leading Shi'ite scribes play a decisive role in the politics of the country, as well as the dominant role of Hizbollah in the Lebanon. Shi'ite groups in a number of countries supporting a Sunni majority have also moved more to the fore. The development that has taken place in Shi'a dominated areas over the last three decades is seen to be based on a Shi'ite tradition of political ideas which has emerged during the 1400 year history of Shi'a Islam. One feature of this development has been the predominant role of the scribes in politics, and their regard for a tradition of political ideas which excludes any sharp distinction between the state/politics and religion. The question is whether this picture agrees with the view held by Shi'ite thinkers regarding the relationship between the state and religion. A backward glance at Shi'a history reveals that no shared view exists among Shi'ite scholars on the subject of politics and this particular relationship. Seen from a history of ideas perspective, it is not possible to speak about an unchanged metahistorical-political discourse which must have coloured all political thought throughout Shi'a history. We find, rather, a broad spectrum of different views among Shi'ite scholars. Another question is whether, aside from the influence of social, historical and political structures and relations, a closer look should also be taken at the importance of the tradition of theological interpretation. Did, for example, the view of the relationship between

  2. 相门出相--试论史浩对史弥远之影响%The Minsisters in the Home of a Statesman:the Influence of Shi Hao on Shi Mi-yuan

    Institute of Scientific and Technical Information of China (English)

    李超

    2016-01-01

    四明史氏为南宋历史上罕有的宰相世家,史浩、史弥远、史嵩之先后为相,尤其是史弥远,执掌朝政更长达26年,令史氏家族显赫一时。史弥远早年虽曾一度师从理学中人,但对理学思想的接受较为有限,他在政治上深受其父史浩影响,他参与政变、对外主和以及用人方式等方面,无不或直接或间接的在效仿乃父。%In the Southern Song Dynasty, the family of Shi is an uncommon prime minister family. Shi Hao, Shi Mi-yuan and Shi Song-zhi successively served as prime ministers. Shi Mi-yuan was typical since he served as a prime minister for 26 years. In his youth Shi Mi-yuan has studied with some parapsychologists, but he did not accept neo-confucianism. His political thought roots from his father Shi Hao. He always imitated his father in taking part in coup attempts, foreign policy and employment approach.

  3. IHF and HU: flexible architects of bent DNA.

    Science.gov (United States)

    Swinger, Kerren K; Rice, Phoebe A

    2004-02-01

    The energetic cost of bending short segments of DNA is very high. This bending is critical for the packaging of DNA and is exploited to regulate many cellular processes. In prokaryotes, IHF and HU are key architectural proteins present at high concentrations. New protein-DNA co-crystal structures, and the adaptation of advanced biophysical and biochemical techniques have led to an improved understanding of how these proteins interact with DNA. These techniques include time-resolved synchrotron X-ray footprinting, differential scanning calorimetry, isothermal titration calorimetry and single-molecule experiments.

  4. Interface reactions between Pd thin films and SiC by thermal annealing and SHI irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Njoroge, E.G., E-mail: eric.njoroge@up.ac.za [Department of Physics, University of Pretoria, Pretoria (South Africa); Theron, C.C. [Department of Physics, University of Pretoria, Pretoria (South Africa); Skuratov, V.A. [Joint Institute for Nuclear Research, Dubna (Russian Federation); Wamwangi, D. [School of Physics, University of Witwatersrand, Johannesburg (South Africa); Hlatshwayo, T.T. [Department of Physics, University of Pretoria, Pretoria (South Africa); Comrie, C.M. [MRD, iThemba LABS, P.O. Box 722, Somerset West 7129 (South Africa); Malherbe, J.B. [Department of Physics, University of Pretoria, Pretoria (South Africa)

    2016-03-15

    The solid-state reactions between Pd thin films and 6H-SiC substrates induced by thermal annealing, room temperature swift heavy ion (SHI) irradiation and high temperature SHI irradiation have been investigated by in situ and real-time Rutherford backscattering spectrometry (RBS) and Grazing incidence X-ray diffraction (GIXRD). At room temperature, no silicides were detected to have formed in the Pd/SiC samples. Two reaction growth zones were observed in the samples annealed in situ and analysed by real time RBS. The initial reaction growth region led to formation of Pd{sub 3}Si or (Pd{sub 2}Si + Pd{sub 4}Si) as the initial phase(s) to form at a temperature of about 450 °C. Thereafter, the reaction zone did not change until a temperature of 640 °C was attained where Pd{sub 2}Si was observed to form in the reaction zone. Kinetic analysis of the initial reaction indicates very fast reaction rates of about 1.55 × 10{sup 15} at cm{sup −2}/s and the Pd silicide formed grew linear with time. SHI irradiation of the Pd/SiC samples was performed by 167 MeV Xe{sup 26+} ions at room temperature at high fluences of 1.07 × 10{sup 14} and 4 × 10{sup 14} ions/cm{sup 2} and at 400 °C at lower fluences of 5 × 10{sup 13} ions/cm{sup 2}. The Pd/SiC interface was analysed by RBS and no SHI induced diffusion was observed for room temperature irradiations. The sample irradiated at 400 °C, SHI induced diffusion was observed to occur accompanied with the formation of Pd{sub 4}Si, Pd{sub 9}Si{sub 2} and Pd{sub 5}Si phases which were identified by GIXRD analysis.

  5. Overexpression of the AtSHI gene in poinsettia, Euphorbia pulcherrima, results in compact plants.

    Directory of Open Access Journals (Sweden)

    M Ashraful Islam

    Full Text Available Euphorbia pulcherrima, poinsettia, is a non-food and non-feed vegetatively propagated ornamental plant. Appropriate plant height is one of the most important traits in poinsettia production and is commonly achieved by application of chemical growth retardants. To produce compact poinsettia plants with desirable height and reduce the utilization of growth retardants, the Arabidopsis SHORT INTERNODE (AtSHI gene controlled by the cauliflower mosaic virus 35S promoter was introduced into poinsettia by Agrobacterium-mediated transformation. Three independent transgenic lines were produced and stable integration of transgene was verified by PCR and Southern blot analysis. Reduced plant height (21-52% and internode lengths (31-49% were obtained in the transgenic lines compared to control plants. This correlates positively with the AtSHI transcript levels, with the highest levels in the most dwarfed transgenic line (TL1. The indole-3-acetic acid (IAA content appeared lower (11-31% reduction in the transgenic lines compared to the wild type (WT controls, with the lowest level (31% reduction in TL1. Total internode numbers, bract numbers and bract area were significantly reduced in all transgenic lines in comparison with the WT controls. Only TL1 showed significantly lower plant diameter, total leaf area and total dry weight, whereas none of the AtSHI expressing lines showed altered timing of flower initiation, cyathia abscission or bract necrosis. This study demonstrated that introduction of the AtSHI gene into poinsettia by genetic engineering can be an effective approach in controlling plant height without negatively affecting flowering time. This can help to reduce or avoid the use of toxic growth retardants of environmental and human health concern. This is the first report that AtSHI gene was overexpressed in poinsettia and transgenic poinsettia plants with compact growth were produced.

  6. 胡适对五四运动的认识与思考%Hu Shi’ s Understanding and Thinking on the May 4th Movement

    Institute of Scientific and Technical Information of China (English)

    周良发

    2015-01-01

    胡适对五四运动的态度,前后有明显变化,由起初赞扬继而深表同情,及至晚年的批评。胡适认为五四运动的爆发除了中国政府的外交失败,还与北京高校学生庆祝一战胜利的游行活动有关,而五四运动的价值在于独立人格的形成,并成为社会发展的主要推力。胡适没有系统研究五四运动,但其言说仍对后世学人的形上致思颇多启发意义。%Hu Shi’ s attitude had a significant change about the May 4th Movement.He praised and showed sympathy in his early life, but later he harshly criticized.He thought that the May 4th outbreak was due to Chinese government diplomatic failure, and it’ s related to Peking university students to celebrate the end of the First World War.The May 4th value lied in the formation of the independent personality, and became the main thrust of social development.Hu Shi didn’ t study May 4th Movement systemical-ly, but what he said had a lot of inspiration significance for the later scholars.

  7. HuPSON: the human physiology simulation ontology.

    Science.gov (United States)

    Gündel, Michaela; Younesi, Erfan; Malhotra, Ashutosh; Wang, Jiali; Li, Hui; Zhang, Bijun; de Bono, Bernard; Mevissen, Heinz-Theodor; Hofmann-Apitius, Martin

    2013-11-22

    Large biomedical simulation initiatives, such as the Virtual Physiological Human (VPH), are substantially dependent on controlled vocabularies to facilitate the exchange of information, of data and of models. Hindering these initiatives is a lack of a comprehensive ontology that covers the essential concepts of the simulation domain. We propose a first version of a newly constructed ontology, HuPSON, as a basis for shared semantics and interoperability of simulations, of models, of algorithms and of other resources in this domain. The ontology is based on the Basic Formal Ontology, and adheres to the MIREOT principles; the constructed ontology has been evaluated via structural features, competency questions and use case scenarios.The ontology is freely available at: http://www.scai.fraunhofer.de/en/business-research-areas/bioinformatics/downloads.html (owl files) and http://bishop.scai.fraunhofer.de/scaiview/ (browser). HuPSON provides a framework for a) annotating simulation experiments, b) retrieving relevant information that are required for modelling, c) enabling interoperability of algorithmic approaches used in biomedical simulation, d) comparing simulation results and e) linking knowledge-based approaches to simulation-based approaches. It is meant to foster a more rapid uptake of semantic technologies in the modelling and simulation domain, with particular focus on the VPH domain.

  8. Design of a detector to study associated charm production in the SHiP beam dump facility

    CERN Document Server

    Iuliano, Antonio; Di Crescenzo, Antonia

    A dedicated experiment has been proposed by the SHiP Collaboration, to study associated charm production and decay of charmed hadrons. In this thesis we report the first design of such an experiment. This work has been carried out within the Naples neutrino group that participates to the SHiP experiment. The aim of the experiment designed in this thesis is to measure the differential associated charm production cross sections with respect to the angular and energy spectra of charmed particles. This measurement could give the acceptance of the SHiP detector for hidden particles and tau neutrinos, which are produced from charmed hadron decays.

  9. SUPPORT FOR HU CFRT SUMMER HIGH SCHOOL FUSION WORKSHOP

    Energy Technology Data Exchange (ETDEWEB)

    Punjabi, Alkesh

    2010-02-09

    Nine summer fusion science research workshops for minority and female high school students were conducted at the Hampton University Center for Fusion Research and Training from 1996 to 2005. Each workshop was of the duration of eight weeks. In all 35 high school students were mentored. The students presented 28 contributed papers at the annual meetings of the American Physical Society Division of Plasma Physics. These contributed papers were very well received by the plasma physics and fusion science research community. The students won a number of prestigious local, state, and national honors, awards, prizes, and scholarships. The notable among these are the two regional finalist positions in the 1999 Siemens-Westinghouse Science and Technology Competitions; 1st Place U.S. Army Award, 2006; 1st Place U.S. Naval Science Award, 2006; Yale Science and Engineering Association Best 11th Grade Project, 2006; Society of Physics Students Book Award, 2006; APS Corporate Minority Scholarship and others. This workshop program conducted by the HU CFRT has been an exemplary success, and served the minority and female students exceptionally fruitfully. The Summer High School Fusion Science Workshop is an immensely successful outreach activity conducted by the HU CFRT. In this workshop, we train, motivate, and provide high quality research experiences to young and talented high school scholars with emphasis on under-represented minorities and female students in fusion science and related areas. The purpose of this workshop is to expose minority and female students to the excitement of research in science at an early stage in their academic lives. It is our hope that this may lead the high school students to pursue higher education and careers in physical sciences, mathematics, and perhaps in fusion science. To our knowledge, this workshop is the first and only one to date, of fusion science for under-represented minorities and female high school students at an HBCU. The faculty

  10. Adaptive and maladaptive expression of the mRNA regulatory protein HuR

    Institute of Scientific and Technical Information of China (English)

    Suman; Govindaraju; Beth; S; Lee

    2013-01-01

    The RNA-binding proteins involved in regulation of mRNA post-transcriptional processing and translation control the fates of thousands of mRNA transcripts and basic cellular processes. The best studied of these, HuR, is well characterized as a mediator of mRNA stability and translation, and more recently, as a factor in nuclear functions such as pre-mRNA splicing. Due to HuR’s role in regulating thousands of mRNA transcripts, including those for other RNA-binding proteins, HuR can act as a master regulator of cell survival and proliferation. HuR itself is subject to multiple post-translationa modifications including regulation of its nucleocytoplasmic distribution. However, the mechanisms that govern HuR levels in the cell have only recently begun to be defined. These mechanisms are critical to cell health, as it has become clear in recent years that aberrant expression of HuR can lead alternately to decreased cell viability or to promotion of pathological proliferation and invasiveness. HuR is expressed as alternate mRNAs that vary in their untranslated regions, leading to differences in transcript stability and translatability. Multiple transcription factors and modulators of mRNA stability that regulate HuR mRNA expression have been identified. In addition, translation of HuR is regulated by numerous microRNAs, several of which have been demonstrated to have anti-tumor properties due to their suppression of HuR expression. This review summarizes the current state of knowledge of the factors that regulate HuR expression, along with the circumstances under which these factors contribute to cancer and inflammation.

  11. Direct determination of absolute configuration: a vibrational circular dichroism study on dimethyl-substituted phenyloxiranes synthesized by Shi epoxidation

    DEFF Research Database (Denmark)

    Fristrup, Peter; Lassen, Peter Rygaard; Tanner, David Ackland;

    2008-01-01

    The three possible dimethylsubstituted phenyloxiranes (cis, trans and geminal) were synthesized in both racemic (mCPBA) and enantiomerically enriched forms (Shi epoxidation) and subjected to a vibrational circular dichroism study. The experimental spectra were compared to theoretical spectra obta...

  12. Fingerprint Recognition Using Local Features and Hu Moments

    Directory of Open Access Journals (Sweden)

    G. Aguilar-Torres

    2012-10-01

    Full Text Available Person identification systems based on fingerprint patterns called Automatic Fingerprint Identification Systems, AFIS,are some of the most widely used biometric methods since they provide a high degree of success. The accuracy ofAFIS is mainly due to some unique characteristics called minutiae, which are points where a curve track finishes,intersects with another curve track, or branches off. During past decades several efficient minutia-based fingerprintrecognition algorithms have been proposed which achieve false recognition rates close to 1%, however, theirrecognition rate may be still improved. To this end, this paper presents a fingerprint recognition method using acombination of the Fast Fourier Transform (FFT with Gabor filters for image enhancement. Next, fingerprintrecognition is carried out using a novel recognition stage based on Local Features and Hu invariant moments forverification.

  13. SUPPORT FOR HU CFRT SUMMER HIGH SCHOOL FUSION WORKSHOP

    Energy Technology Data Exchange (ETDEWEB)

    Punjabi, Alkesh

    2010-02-09

    Nine summer fusion science research workshops for minority and female high school students were conducted at the Hampton University Center for Fusion Research and Training from 1996 to 2005. Each workshop was of the duration of eight weeks. In all 35 high school students were mentored. The students presented 28 contributed papers at the annual meetings of the American Physical Society Division of Plasma Physics. These contributed papers were very well received by the plasma physics and fusion science research community. The students won a number of prestigious local, state, and national honors, awards, prizes, and scholarships. The notable among these are the two regional finalist positions in the 1999 Siemens-Westinghouse Science and Technology Competitions; 1st Place U.S. Army Award, 2006; 1st Place U.S. Naval Science Award, 2006; Yale Science and Engineering Association Best 11th Grade Project, 2006; Society of Physics Students Book Award, 2006; APS Corporate Minority Scholarship and others. This workshop program conducted by the HU CFRT has been an exemplary success, and served the minority and female students exceptionally fruitfully. The Summer High School Fusion Science Workshop is an immensely successful outreach activity conducted by the HU CFRT. In this workshop, we train, motivate, and provide high quality research experiences to young and talented high school scholars with emphasis on under-represented minorities and female students in fusion science and related areas. The purpose of this workshop is to expose minority and female students to the excitement of research in science at an early stage in their academic lives. It is our hope that this may lead the high school students to pursue higher education and careers in physical sciences, mathematics, and perhaps in fusion science. To our knowledge, this workshop is the first and only one to date, of fusion science for under-represented minorities and female high school students at an HBCU. The faculty

  14. Overexpression of the AtSHI gene in poinsettia, Euphorbia pulcherrima, results in compact plants

    DEFF Research Database (Denmark)

    Islam, M. Ashraful; Lütken, Henrik Vlk; Haugslien, Sissel

    2013-01-01

    Euphorbia pulcherrima, poinsettia, is a non-food and non-feed vegetatively propagated ornamental plant. Appropriate plant height is one of the most important traits in poinsettia production and is commonly achieved by application of chemical growth retardants. To produce compact poinsettia plants...... of the AtSHI gene into poinsettia by genetic engineering can be an effective approach in controlling plant height without negatively affecting flowering time. This can help to reduce or avoid the use of toxic growth retardants of environmental and human health concern. This is the first report that At...... integration of transgene was verified by PCR and Southern blot analysis. Reduced plant height (21–52%) and internode lengths (31–49%) were obtained in the transgenic lines compared to control plants. This correlates positively with the AtSHI transcript levels, with the highest levels in the most dwarfed...

  15. An Analysis of Sexual Abnormal Psychology of Shi Xiu Described by Shi Zhecun%施蛰存笔下石秀性变态心理解析

    Institute of Scientific and Technical Information of China (English)

    杜九霞

    2011-01-01

    施蛰存是中国现代文学史上独辟蹊径创作了大量心理分析小说的一位作家。运用弗洛依德的精神分析理论对其小说《石秀》进行解读,进而探讨石秀性变态心理及其原因。认为,施蛰存笔下的石秀从《水浒传》里的神坛走了下来,变成了“人”,然而却是一个人格扭曲、心理变态的形象。%Shi Zhecun is a unique writer in modem Chinese literary history, who created a lot of psycho- logical analysis novels on his own. By using Freud's psychoanalytic theory, this paper interprets his novel "Shi Xiu" , and then discuses the sexual abnormal psy

  16. SHiP: a new multipurpose beam-dump experiment at the SPS

    Science.gov (United States)

    Dijkstra, H. B.

    2016-11-01

    SHiP is an experiment to look for very weakly interacting particles at a new to be constructed beam-dump facility at the CERN SPS. The SHiP Technical Proposal has been submitted to the CERN SPS Committee in April 2015. The 400 GeV/c proton beam extracted from the SPS will be dumped on a heavy target with the aim of integrating 2 × 1020 proton on target in five years. A detector located downstream of the target, based on a long vacuum tank followed by a spectrometer and particle identification detectors, will allow probing a variety of models with light long-lived exotic particles and masses below a few GeV/c2. The main focus will be the physics of the so-called Hidden Portals, i.e. search for Dark Photons, Light scalars and pseudo-scalars, and Heavy Neutral Leptons (HNL). The sensitivity to HNL will allow for the first time to probe, in the mass range between the kaon and the charm meson mass, a coupling range for which Baryogenesis and active neutrino masses could also be explained. Integrated in SHiP is an Emulsion Cloud Chamber, already used in the OPERA experiment, which will allow to study active neutrino cross-sections and angular distributions. In particular SHiP can distinguish between vτ and v¯τ, and their deep inelastic scattering cross sections will be measured with statistics three orders of magnitude larger than currently available.

  17. A Meta Analysis and Hierarchical Classification of HU-Based Atherosclerotic Plaque Characterization Criteria

    NARCIS (Netherlands)

    Kristanto, Wisnumurti; van Ooijen, Peter M. A.; Jansen-van der Weide, Marijke C.; Vliegenthart, Rozemarijn; Oudkerk, Matthijs

    2013-01-01

    Background: Many computed tomography (CT) studies have reported that lipid-rich, presumably rupture-prone atherosclerotic plaques can be characterized according to their Hounsfield Unit (HU) value. However, the published HU-based characterization criteria vary considerably. The present study aims to

  18. HuD Regulates Coding and Noncoding RNA to Induce APP→Aβ Processing

    Directory of Open Access Journals (Sweden)

    Min-Ju Kang

    2014-06-01

    Full Text Available The primarily neuronal RNA-binding protein HuD is implicated in learning and memory. Here, we report the identification of several HuD target transcripts linked to Alzheimer’s disease (AD pathogenesis. HuD interacted with the 3′ UTRs of APP mRNA (encoding amyloid precursor protein and BACE1 mRNA (encoding β-site APP-cleaving enzyme 1 and increased the half-lives of these mRNAs. HuD also associated with and stabilized the long noncoding (lncRNA BACE1AS, which partly complements BACE1 mRNA and enhances BACE1 expression. Consistent with HuD promoting production of APP and APP-cleaving enzyme, the levels of APP, BACE1, BACE1AS, and Aβ were higher in the brain of HuD-overexpressing mice. Importantly, cortex (superior temporal gyrus from patients with AD displayed significantly higher levels of HuD and, accordingly, elevated APP, BACE1, BACE1AS, and Aβ than did cortical tissue from healthy age-matched individuals. We propose that HuD jointly promotes the production of APP and the cleavage of its amyloidogenic fragment, Aβ.

  19. Study of nu-tau properties with the SHiP experiment

    CERN Document Server

    AUTHOR|(CDS)2209555

    The SHiP experiment (Search for Hidden Particles) is a beam dump experiment proposed at the CERN SPS with the submission of a Technical Proposal in April 2015. SHiP aims at the observation of long lived particles very weakly coupled with ordinary matter. These particles are mostly produced in the decay of charmed hadrons whose production is therefore enhanced through the definition of the characteristics of both the beam and the proton target. This makes the SHiP experiment a Standard Model neutrino factory too, in particular of tau neutrinos produced by the Ds decay chain. My studies have mainly focused on the design of the neutrino detector and on the evaluation of its performances. The Neutrino Detector is placed in a magnetic field and it exploits the Emulsion Cloud Chamber Technology with the micrometric position resolution needed to disentangle the tau lepton decay vertex from the neutrino interaction vertex. This peculiarity, together with the high electron identification efficiency, makes this det...

  20. SHiP: a new multipurpose beam-dump experiment at the SPS

    Directory of Open Access Journals (Sweden)

    Dijkstra H.B.

    2016-01-01

    to be constructed beam-dump facility at the CERN SPS. The SHiP Technical Proposal has been submitted to the CERN SPS Committee in April 2015. The 400 GeV/c proton beam extracted from the SPS will be dumped on a heavy target with the aim of integrating 2 × 1020 proton on target in five years. A detector located downstream of the target, based on a long vacuum tank followed by a spectrometer and particle identification detectors, will allow probing a variety of models with light long-lived exotic particles and masses below a few GeV/c2. The main focus will be the physics of the so-called Hidden Portals, i.e. search for Dark Photons, Light scalars and pseudo-scalars, and Heavy Neutral Leptons (HNL. The sensitivity to HNL will allow for the first time to probe, in the mass range between the kaon and the charm meson mass, a coupling range for which Baryogenesis and active neutrino masses could also be explained. Integrated in SHiP is an Emulsion Cloud Chamber, already used in the OPERA experiment, which will allow to study active neutrino cross-sections and angular distributions. In particular SHiP can distinguish between vτ and v̄τ, and their deep inelastic scattering cross sections will be measured with statistics three orders of magnitude larger than currently available.

  1. Development of a Summarized Health Index (SHI for use in predicting survival in sea turtles.

    Directory of Open Access Journals (Sweden)

    Tsung-Hsien Li

    Full Text Available Veterinary care plays an influential role in sea turtle rehabilitation, especially in endangered species. Physiological characteristics, hematological and plasma biochemistry profiles, are useful references for clinical management in animals, especially when animals are during the convalescence period. In this study, these factors associated with sea turtle surviving were analyzed. The blood samples were collected when sea turtles remained alive, and then animals were followed up for surviving status. The results indicated that significantly negative correlation was found between buoyancy disorders (BD and sea turtle surviving (p < 0.05. Furthermore, non-surviving sea turtles had significantly higher levels of aspartate aminotranspherase (AST, creatinine kinase (CK, creatinine and uric acid (UA than surviving sea turtles (all p < 0.05. After further analysis by multiple logistic regression model, only factors of BD, creatinine and UA were included in the equation for calculating summarized health index (SHI for each individual. Through evaluation by receiver operating characteristic (ROC curve, the result indicated that the area under curve was 0.920 ± 0.037, and a cut-off SHI value of 2.5244 showed 80.0% sensitivity and 86.7% specificity in predicting survival. Therefore, the developed SHI could be a useful index to evaluate health status of sea turtles and to improve veterinary care at rehabilitation facilities.

  2. Immune response in lung cancer mouse model mimics human anti-Hu reactivity

    Science.gov (United States)

    Kazarian, Meleeneh; Calbo, Joaquim; Proost, Natalie; Carpenter, Catherine L.; Berns, Anton; Laird-Offringa, Ite A.

    2009-01-01

    Most patients with paraneoplastic encephalomyelitis/sensory neuronopathy PEM/SN have small-cell lung cancer (SCLC) and develop antibodies against neuronal-specific Hu proteins, which are abnormally expressed in the tumor. Anti-Hu reactivity is present in ~16% of SCLC patients without PEM/SN. Here we test the hypothesis that engineered SCLC-prone mice may exhibit anti-Hu reactivity. We show that tumors from SCLC-prone mice misexpress Hu proteins, and 14% of mice harbor anti-Hu antibodies. Mice appear to show reactivity prior to clinical diagnosis of SCLC. This mouse model system will be useful to study SCLC-associated autoimmunity, its diagnostic value, and the potential protective role of oncoantigen-directed autoantibodies. PMID:19765830

  3. HuR knockdown changes the oncogenic potential of oral cancer cells.

    Science.gov (United States)

    Kakuguchi, Wataru; Kitamura, Tetsuya; Kuroshima, Takeshi; Ishikawa, Makoto; Kitagawa, Yoshimasa; Totsuka, Yasunori; Shindoh, Masanobu; Higashino, Fumihiro

    2010-04-01

    HuR binds to AU-rich element-containing mRNA to protect them from rapid degradation. Here, we show that knockdown of HuR changes the oncogenic properties of oral cancer cells. Oral squamous cell carcinoma cell lines, HSC-3 and Ca9.22, which express HuR protein and cytoplasmic AU-rich element mRNA more abundantly than normal cells, were subjected to HuR knockdown. In the HuR-knockdown cancer cells, the cytoplasmic expression of c-fos, c-myc, and COX-2 mRNAs was inhibited compared with those in cells that had been transfected with a control small interfering RNA, and the half-lives of these mRNAs were shorter than those of their counterparts in the control cells. HuR-knockdown cells failed to make colonies in soft agar, suggesting that the cells had lost their ability for anchorage-independent cell growth. Additionally, the motile and invasive activities of the cells decreased remarkably by HuR knockdown. Furthermore, the expression of cell cycle-related proteins, such as cyclin A, cyclin B1, cyclin D1, and cyclin-dependent kinase 1, was reduced in HuR-knockdown cancer cells, and HuR bound to cdk1 mRNA to stabilize it. These findings suggest that HuR knockdown changes the features of oral cancer cells, at least in part, by affecting their cell cycle and shows potential as an effective therapeutic approach.

  4. HuR contributes to Hepatic Stellate Cell activation and liver fibrosis

    Science.gov (United States)

    Woodhoo, A.; Iruarrizaga-Lejarreta, M.; Beraza, N.; García-Rodríguez, J.L.; Embade, N.; Fernández-Ramos, D.; Matinez-Lopez, N.; Gutiérrez, Virginia; Arteta, B.; Caballeria, J.; Lu, S.C.; Mato, J.M.; Varela-Rey, M.; Martinez-Chantar, M.L.

    2012-01-01

    RNA-binding proteins (RBPs) play a major role in control of mRNA turnover and translation rates. We examined the role of the RBP human antigen R (HuR) during cholestatic liver injury and hepatic stellate cells (HSC) activation. HuR silencing attenuated fibrosis development in vivo after BDL, reducing liver damage, oxidative stress, inflammation, and collagen and α-SMA (α-smooth muscle actin) expression. HuR expression increased in activated HSC from BDL mice and during HSC activation in vitro, and HuR silencing markedly reduced HSC activation. HuR regulated platelet-derived growth factor (PDGF)-induced proliferation and migration, and controlled expression of several mRNAs involved in these processes (Actin, MMP9, Cyclin D1 and B1). These functions of HuR were linked to its abundance and cytoplasmic localisation, controlled by PDGF, via ERK and PI3K activation, and ERK-LKB1 activation respectively. More importantly, we identified the tumor suppressor LKB1 as a novel downstream target of PDGF-induced ERK activation in HSC. HuR also controlled transforming growth factor beta (TGF-β-induced profibrogenic actions by regulating expression of TGF-β, α-SMA, and p21. This was likely due to an increased cytoplasmic localisation of HuR, controlled by TGF-β-induced p38 MAPK activation. Finally, we found that HuR and LKB1 (Ser428) levels were highly expressed in activated HSC in human cirrhotic samples. Conclusion: Our results show that HuR is important for pathogenesis of liver fibrosis development in the cholestatic injury model, for HSC activation, and for the response of activated HSC to PDGF and TGF-β. PMID:22576182

  5. Regulation of the mRNA-binding protein HuR by posttranslational modification: spotlight on phosphorylation.

    Science.gov (United States)

    Eberhardt, Wolfgang; Doller, Anke; Pfeilschifter, Josef

    2012-06-01

    The ubiquitous mRNA-binding protein human antigen R (HuR) and its neuronal relatives (HuB, HuC, HuD) participate in the post-transcriptional regulation of many AU-rich element-bearing mRNAs. In addition to its originally described role in controlling mRNA decay, the binding of HuR to target mRNAs can affect many aspects of mRNA processing including splicing, polyadenylation, intracellular trafficking, translation and modulation of mRNA repression by miRNAs. In accordance to the growing list of signalling events which are involved in regulating these different HuR functions, recent data implicate that posttranslational modification, namely protein kinase-triggered phosphorylation of HuR plays a crucial role in connecting extracellular signal inputs to a specific post-transcriptional program by HuR. Notably, in addition to directly targeting HuR functions, posttranslational modifications of HuR have a major impact on the sequestration and binding to various HuR ligand proteins as has been demonstrated e.g. for the 14-3-3 chaperones. However, the detailed mechanisms of how a specific modification of HuR coordinates different aspects in HuR regulation are currently poorly understood. Due to the fact that most of the described HuR activities are closely related to its subcellular localization and the binding to cargo mRNA, this review will focus on these aspects of HuR functions and their control by posttranslational modification, particularly by HuR phosphorylations by different protein kinases.

  6. Structural characterization of the maytansinoid–monoclonal antibody immunoconjugate, huN901–DM1, by mass spectrometry

    National Research Council Canada - National Science Library

    Wang, Lintao; Amphlett, Godfrey; Blättler, Walter A; Lambert, John M; Zhang, Wei

    2005-01-01

    ...‐specific drug delivery. The immunoconjugate, huN901–DM1, composed of the humanized monoclonal IgG 1 antibody, huN901, and the maytansinoid drug, DM1, is being tested in clinical trials to treat small cell lung carcinoma (SCLC). huN901...

  7. Measurement of hard tissue density of head phantom based on the HU by using CBCT

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Moon Sun; Kang, Dong Wan; Kim, Jae Duk [School of Dentistry, Chosun University, Gwangju (Korea, Republic of)

    2009-09-15

    The purpose of this study was to determine a conversion coefficient for Hounsfield Units(HU) to material density (g cm{sup -3}) obtained from cone-beam computed tomography (CBMercuRay{sup TM}) data and to measure the hard tissue density based on the Hounsfield scale on dental head phantom. CT Scanner Phantom (AAPM) equipped with CT Number Insert consists of five cylindrical pins of materials with different densities and teflon ring was scanned by using the CBMercuRay{sup TM} (Hitachi, Tokyo, Japan) volume scanner. The raw data were converted into DICOM format and the HU of different areas of CT number insert measured by using CBWorks{sup TM}. Linear regression analysis and Student t-test were performed statistically. There was no significant difference (P>0.54) between real densities and measured densities. A linear regression was performed using the density, {rho} (g cm{sup -3}), as the dependent variable in terms of the HU (H). The regression equation obtained was {rho}=0.00072 H-0.01588 with an R2 value of 0.9968. Density values based on the Hounsfield scale was 1697.1 {+-} 24.9 HU in cortical bone, 526.5 {+-} 44.4 HU in trabecular bone, 2639.1 {+-} 48.7 HU in enamel, 1246.1 {+-} 39.4 HU in dentin of dental head phantom. CBCT provides an effective option for determination of material density expressed as Hounsfield Units.

  8. On the ephemeris of the eclipsing polar HU Aquarii

    CERN Document Server

    Schwope, A D

    2014-01-01

    The magnetic cataclysmic variable HU Aquarii displayed pronounced quasi-periodic modulations of its eclipse timing. These were interpreted in terms of the light-travel time (LTT) effect caused by a circumbinary planet or planetary system. We report new photometric observations that revealed another precise eclipse timing for the October 2013 epoch, the first obtained in a high accretion state after many years in low or intermediate states. The eclipse was observed to occur earlier by 95.3 +- 2.0 s or 62.8 +- 2.0 s than expected for an assumed linear or quadratic ephemeris, respectively. The implied apparent strong evolution of the orbital period calls for a revision of the current planetary model or the planetary parameters. The object deserves further monitoring to uncover the true nature of the observed variability and to constrain the properties of the proposed planet or planetary system. The new observations prove that advanced amateur equipment can successfully be used in the growing field of planet sear...

  9. HuR contributes to Hepatic Stellate Cell activation and liver fibrosis

    OpenAIRE

    Woodhoo, A.; Iruarrizaga-Lejarreta, M.; Beraza, N.; García-Rodríguez, J.L.; Embade, N.; Fernández-Ramos, D.; Matinez-Lopez, N.; Gutiérrez, Virginia; Arteta, B; Caballeria, J.; Lu, S.C. (Shelly C.); Mato, J.M. (José María); Varela-Rey, M.; Martinez-Chantar, M.L.

    2012-01-01

    RNA-binding proteins (RBPs) play a major role in control of mRNA turnover and translation rates. We examined the role of the RBP human antigen R (HuR) during cholestatic liver injury and hepatic stellate cells (HSC) activation. HuR silencing attenuated fibrosis development in vivo after BDL, reducing liver damage, oxidative stress, inflammation, and collagen and α-SMA (α-smooth muscle actin) expression. HuR expression increased in activated HSC from BDL mice and during HSC activation in vitro...

  10. 关于苏轼并非音律家的探究%Su Shi Is not A Musician

    Institute of Scientific and Technical Information of China (English)

    刘明哥

    2016-01-01

    Academia has long been a debate on the question of whether the ci‐poetry of Sushi in music .Various opinions vary .Someone said that Su Shi understand some of the music ,also somebody said that Su Shi is a musician . There are differences of temperament to understand the melody .From the aspects the rules .The ci‐poetry of Su Shi clearly meets the requirements ,but from the aspects of melody ,many of the ci‐poetry of Su Shi do not conform to the melody ,so Su Shi is not a musician .%苏轼词是否和音律这一问题,学界争论由来已久,各家众说纷纭,有人说苏轼只是略懂音律,也有人说苏轼精通音律,是音律家。各家对于音律的理解都存在着分歧,在格律方面苏轼作词显然符合要求,但从音律方面来讲,苏轼的词有很多不和音律的现象,不能说苏轼是音律家。

  11. SHiP: a new facility to search for heavy neutrinos and study $\

    CERN Document Server

    De Serio, Marilisa

    2016-01-01

    SHiP (Search for Hidden Particles) is a newly designed fixed target facility, proposed at the CERN SPS accelerator, with the aim of complementing searches for New Physics at LHC by searching for light long-lived exotic particles with masses below a few GeV/c2. The sensitivity to Heavy Neutrinos will allow for the first time probing a region of the parameter space where Baryogenesis and active neutrino masses and oscillation could also be explained. A dedicated detector, based on OPERA-like bricks, will provide the first observation of the tau anti-neutrino. Moreover, $\

  12. SHI Zai-xiang (史载祥)--An Expert of Cardiology in Integrative Medicine

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ Dr. SHI Zai-xiang was born in TengzhouCity of Shandong Province on June 18, 1942.He was graduated from Nanjing University of TCM in 1965. From 1965 to 1978, he has been working in Nantong Municipal TCM Hospital, where he learnt from and followed Prof. ZHU Liang-chun, a well-known veteran TCM doctor. After that he finished his Master's degree courses in cardiovascular field of integrative Chinese and Western medicine (ICWM) in Beijing University of Chinese Medicine in 1981, and has been holding the post in Beijing China-Japan Friendship Hospital until now.

  13. Surface Morphology Dependent Copper Sulphide Ammonia Gas Sensor Working at Room Temperature: Effect of SHI Irradiation

    Directory of Open Access Journals (Sweden)

    Ramphal Sharma

    2009-02-01

    Full Text Available We report the synthesis of copper sulphide (CuS nano-pillars on copper sulphide thin film surface by using swift heavy ion (SHI irradiation. Thin films of CuS are irradiated with 100 MeV gold ions at fluence varying from 1011 to 5 ´ 1012 ions/cm2. These nanostructures grown on the surface of copper sulphide has been used for the detection of ammonia gas at room temperature. The time dependent surface conductance measurements show the utility of copper sulphide for the detection of ammonia. It is observed that the response of the material is highly influenced by the irradiation fluence.

  14. Surface Morphology Dependent Copper Sulphide Ammonia Gas Sensor Working at Room Temperature: Effect of SHI Irradiation

    OpenAIRE

    Ramphal Sharma; Sagade, Abhay A.; J. C. Vyas; P. K. Nema; Anil Ghule; Sung-Hwan Han

    2009-01-01

    We report the synthesis of copper sulphide (CuS) nano-pillars on copper sulphide thin film surface by using swift heavy ion (SHI) irradiation. Thin films of CuS are irradiated with 100 MeV gold ions at fluence varying from 1011 to 5 ´ 1012 ions/cm2. These nanostructures grown on the surface of copper sulphide has been used for the detection of ammonia gas at room temperature. The time dependent surface conductance measurements show the utility of copper sulphide for the detection of ammonia. ...

  15. Ms HU Ling-xiang's Experience in Treating Blood Stasis by Acupuncture Balancing Method

    Institute of Scientific and Technical Information of China (English)

    FU Jia; LI Ji; ZHANG Cai-rong

    2005-01-01

    @@ HU Ling-xiang, 60, is a chief doctor and supervisor of postgraduates of master degree. She specializes in the syndrome differentiation and treatment of different kinds of chronic and difficult disorders from the perspective of blood stasis theory.

  16. Zebrafish transgenic line huORFZ is an effective living bioindicator for detecting environmental toxicants.

    Directory of Open Access Journals (Sweden)

    Hung-Chieh Lee

    Full Text Available Reliable animal models are invaluable for monitoring the extent of pollution in the aquatic environment. In this study, we demonstrated the potential of huORFZ, a novel transgenic zebrafish line that harbors a human upstream open reading frame of the chop gene fused with GFP reporter, as an animal model for monitoring environmental pollutants and stress-related cellular processes. When huORFZ embryos were kept under normal condition, no leaked GFP signal could be detected. When treated with hazardous chemicals, including heavy metals and endocrine-disrupting chemicals near their sublethal concentrations (LC50, huORFZ embryos exhibited different tissue-specific GFP expression patterns. For further analysis, copper (Cu2+, cadmium (Cd2+ and Chlorpyrifos were applied. Cu2+ triggered GFP responses in skin and muscle, whereas Cd2+ treatment triggered GFP responses in skin, olfactory epithelium and pronephric ducts. Moreover, fluorescence intensity, as exhibited by huORFZ embryos, was dose-dependent. After surviving treated embryos were returned to normal condition, survival rates, as well as TUNEL signals, returned to pretreatment levels with no significant morphological defects observed. Such results indicated the reversibility of treatment conditions used in this study, as long as embryos survived such conditions. Notably, GFP signals decreased along with recovery, suggesting that GFP signaling of huORFZ embryos likely reflected the overall physiological condition of the individual. To examine the performance of the huORFZ line under real-world conditions, we placed huORFZ embryos in different river water samples. We found that the huORFZ embryos correctly detected the presence of various kinds of pollutants. Based on these findings, we concluded that such uORFchop-based system can be integrated into a first-line water alarm system monitoring the discharge of hazardous pollutants.

  17. Hu'rthle Cell Thyroid Adenoma with an Eggshell Calcification: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dong Gil; Lee, Sang Kwon; Choe, Mi Sun; Chang, Hyuk Won; Rho, Byung Hak [Dongsan Medical Center, Keimyung University School of Medicine, Daegu (Korea, Republic of)

    2009-09-15

    We report here a case of Hu'rthle cell adenoma with eggshell calcification that presented as a thyroid incidentaloma on ultrasonography (US) in a 58-year-old woman. The mass was hypoechoic with continuous eggshell calcification and intranodular vascularity as seen on gray-scale and power Doppler (PD) US. Hu 'rthle cell adenoma should be considered in the differential diagnosis of a thyroid nodule with eggshell calcification

  18. Temperament, disordered attachment, and parental sensitivity in foster care: differential findings on attachment security for shy children.

    Science.gov (United States)

    De Schipper, J Clasien; Oosterman, Mirjam; Schuengel, Carlo

    2012-01-01

    In a foster care sample, the moderating effect of temperamental shyness on the association between parental sensitivity and attachment quality was tested. The foster parents of 59 foster children (age M = 57 months, SD = 16.4) filled out the Child Behavior Questionnaire. To control for confounds, symptoms of inhibited and disinhibited disordered attachment were derived from the Disturbances of Attachment Interview. The Strange Situation Procedure as well as a 15 minute parent-child interaction task were administered. Analyses indicated an interaction effect between parental perceptions of shyness and parental sensitivity for attachment quality. Shy children who had more sensitive foster parents were more often securely attached. For less shy children, no differences in attachment security were found in relation to the foster parents' sensitivity. These results are partially consistent with the differential susceptibility hypothesis. Shy children may benefit more from more sensitive foster parents when entering foster care.

  19. Neuroprotective effects of the cannabinoid agonist HU210 on retinal degeneration.

    Science.gov (United States)

    Lax, Pedro; Esquiva, Gema; Altavilla, Cesare; Cuenca, Nicolás

    2014-03-01

    Cannabinoids have been demonstrated to exert neuroprotective effects on different types of neuronal insults. Here we have addressed the therapeutic potential of the synthetic cannabinoid HU210 on photoreceptor degeneration, synaptic connectivity and functional activity of the retina in the transgenic P23H rat, an animal model for autosomal dominant retinitis pigmentosa (RP). In P23H rats administered with HU210 (100 μg/kg, i.p.) from P24 to P90, ERG recordings showed an amelioration of vision loss, as compared to vehicle-administered animals. Under scotopic conditions, the maximum a-wave amplitudes recorded at P60 and P90 were higher in HU210-treated animals, as compared to the values obtained in untreated animals. The scotopic b-waves were significantly higher in treated animals than in untreated rats at P30, P60 and P90. This attenuation of visual deterioration correlated with a delay in photoreceptor degeneration and the preservation of retinal cytoarchitecture. HU210-treated animals had 40% more photoreceptors than untreated animals. Presynaptic and postsynaptic elements, as well as the synaptic contacts between photoreceptors and bipolar or horizontal cells, were also preserved in HU210-treated P23H rats. These results indicate that HU210 preserves cone and rod structure and function, together with their contacts with postsynaptic neurons, in P23H rats. These data suggest that cannabinoids are potentially useful to delay retinal degeneration in RP patients.

  20. Pertussis toxin B-oligomer inhibits HIV infection and replication in hu-PBL-SCID mice.

    Science.gov (United States)

    Lapenta, Caterina; Spada, Massimo; Santini, Stefano M; Racca, Sara; Dorigatti, Fernanda; Poli, Guido; Belardelli, Filippo; Alfano, Massimo

    2005-04-01

    Bordetella pertussis toxin B-oligomer (PTX-B) has been shown to inhibit HIV infection and replication in vitro. The potential anti-viral effect of PTX-B was tested here in an in vivo surrogate model of HIV infection, i.e. SCID mice reconstituted with human peripheral blood leukocytes (PBL) (hu-PBL-SCID) and infected with a CCR5-dependent (R5) HIV-1 strain. SCID mice inoculated intra-peritoneal (i.p.) with PTX-B and then infected with the R5 strain SF-162 were sacrificed 7 days later and analyzed for human PBL (hu-PBL) lymphoid tissue reconstitution, infection of hu-PBL, plasma viremia and viral rescue from ex vivo-cultivated i.p. hu-PBL. Unlike mice treated with 500 ng per animal of PTX-B showing no evidence of viral inhibition, daily administration of PTX-B (50 ng per mouse) strongly inhibited virus infection and replication, as determined by undetectable viremia, absence of infected hu-PBL and lack of rescue of infectious HIV in most animals. Furthermore, PTX-B injection 2 h before and twice after infection prevented HIV-1 infection and replication in all (10/10) tested animals. Thus, PTX-B potently inhibited virus infection and replication in hu-PBL-SCID mice, supporting the hypothesis that it may represent a new pharmacological agent against HIV-1 infection.

  1. Different modes of interaction by TIAR and HuR with target RNA and DNA.

    Science.gov (United States)

    Kim, Henry S; Wilce, Matthew C J; Yoga, Yano M K; Pendini, Nicole R; Gunzburg, Menachem J; Cowieson, Nathan P; Wilson, Gerald M; Williams, Bryan R G; Gorospe, Myriam; Wilce, Jacqueline A

    2011-02-01

    TIAR and HuR are mRNA-binding proteins that play important roles in the regulation of translation. They both possess three RNA recognition motifs (RRMs) and bind to AU-rich elements (AREs), with seemingly overlapping specificity. Here we show using SPR that TIAR and HuR bind to both U-rich and AU-rich RNA in the nanomolar range, with higher overall affinity for U-rich RNA. However, the higher affinity for U-rich sequences is mainly due to faster association with U-rich RNA, which we propose is a reflection of the higher probability of association. Differences between TIAR and HuR are observed in their modes of binding to RNA. TIAR is able to bind deoxy-oligonucleotides with nanomolar affinity, whereas HuR affinity is reduced to a micromolar level. Studies with U-rich DNA reveal that TIAR binding depends less on the 2'-hydroxyl group of RNA than HuR binding. Finally we show that SAXS data, recorded for the first two domains of TIAR in complex with RNA, are more consistent with a flexible, elongated shape and not the compact shape that the first two domains of Hu proteins adopt upon binding to RNA. We thus propose that these triple-RRM proteins, which compete for the same binding sites in cells, interact with their targets in fundamentally different ways.

  2. 广式腊肠风味研究进展%Advances of research on flavor of guang - shi sausage

    Institute of Scientific and Technical Information of China (English)

    李伟雄

    2012-01-01

    The major aromas, the formation mechanism of flavor, the development direction and the prospect of guang - shi sausage were expounded in this paper, to provide a basis for guang - shi sausage processing.%阐述了广式腊肠风味主体及其风味物质形成机理的研究进展,并对其发展方向和前景进行了展望,为广式腊肠的加工提供依据。

  3. SHiP: a new multipurpose beam-dump experiment at the SPS.

    CERN Document Server

    Dijkstra, Hans

    2016-01-01

    SHiP is an experiment to look for very weakly interacting particles at a new to be constructed beam-dum p facility at the CERN SPS. The SHiP Technical Proposal has been submitted to the CERN SPS Committee in April 2015. The 400 GeV/c proton beam extracted from the SPS will be dumped on a heavy target with the aim of integ rating $2\\times 10^{20}$ proton on target in five years. A detector located downstream of the target, based on a long vacuum tank followed by a spectrometer and particle identification detectors, will allow probing a variety of models with light long-lived exotic particles and masses below a few GeV/c$^2$. The main focus will be the physics of the so-called Hidden Portals, i.e. search for Dark Photons, Light scalars and pseudo-scalars, and Heavy Neutral Leptons (HNL). The sensitivity to HNL will allow for the first time to probe, in the mass range between the kaon and the charm meson mass, a coupling range for which Baryogenesis and active neutrino masses could also be explained...

  4. Making Muslim babies: IVF and gamete donation in Sunni versus Shi'a Islam.

    Science.gov (United States)

    Inhorn, Marcia C

    2006-12-01

    Medical anthropological research on science, biotechnology, and religion has focused on the "local moral worlds" of men and women as they make difficult decisions regarding their health and the beginnings and endings of human life. This paper focuses on the local moral worlds of infertile Muslims as they attempt to make, in the religiously correct fashion, Muslim babies at in vitro fertilization (IVF) clinics in Egypt and Lebanon. As early as 1980, authoritative fatwas issued from Egypt's famed Al-Azhar University suggested that IVF and similar technologies are permissible as long as they do not involve any form of third-party donation (of sperm, eggs, embryos, or uteruses). Since the late 1990s, however, divergences in opinion over third-party gamete donation have occurred between Sunni and Shi'ite Muslims, with Iran's leading ayatollah permitting gamete donation under certain conditions. This Iranian fatwa has had profound implications for the country of Lebanon, where a Shi'ite majority also seeks IVF services. Based on three periods of ethnographic research in Egyptian and Lebanese IVF clinics, this paper explores official and unofficial religious discourses surrounding the practice of IVF and third-party donation in the Muslim world, as well as the gender implications of gamete donation for Muslim marriages.

  5. Effects of Language Context on Ratings of Shy and Unsociable Behaviors in English Language Learning Children

    Science.gov (United States)

    Ash, Andrea C.; Rice, Mabel L.; Redmond, Sean M.

    2014-01-01

    Purpose The primary goal of this study was to explore the effect of the language context on the socially withdrawn behaviors of school aged-children who are English Language Learners (ELLs) from middle to high SES backgrounds. This is one of the first studies to address the frequently confused concepts of shyness and unsociability as independent constructs within the ELL population. This study also investigated the feasibility of an experimental parent and child questionnaire that examines shyness and unsociability across native and English speaking contexts. Method Children and parents (34 ELL and 37 native English speaking) were administered an experimental questionnaire examining shy and unsociable behavior in native language and English-speaking contexts. Results Parents and children from the ELL group reported significantly higher ratings of shy behavior in English versus native language contexts, whereas unsociable ratings did not differ across language contexts. Conclusions Shyness and unsociability are distinguishable behaviors in ELL children and these constructs should be considered when examining withdrawal. Additionally, examining ELL children’s behavior across language contexts provides a valuable method for investigating language influenced behavioral problems. This study demonstrates the need for service providers to evaluate behavior across subtype and language context before pathologizing withdrawal in ELL children. PMID:24687767

  6. Congratulations to Academician Changxu Shi on the Occasion of His Winning the 2010 Chinese Science & Technology Grand Prize%Congratulations to Academician Changxu Shi on the Occasion of His Winning the 2010 Chinese Science & Technology Grand Prize

    Institute of Scientific and Technical Information of China (English)

    Ju Li; Kai Chen; Zhiwei Shan; Guanjun Qiao; Jun Sun; Evan Ma

    2011-01-01

    On behalf of friends and colleagues at MIT and Johns Hopkins University, as well as students and faculty at the Center for Advancing Materials Performance from the Nanoscale (CAMP-Nano) at Xi'an Jiaotong University, we would like to express our hearty congratulations to Academician Shi on the occasion of his winning the highest science and technology award of China.

  7. Functional characterization of the HuR:CD83 mRNA interaction.

    Directory of Open Access Journals (Sweden)

    Dorothea Pieper

    Full Text Available Maturation of dendritic cells (DC is characterized by expression of CD83, a surface protein that appears to be necessary for the effective activation of naïve T-cells and T-helper cells by DC. Lately it was shown that CD83 expression is regulated on the posttranscriptional level by interaction of the shuttle protein HuR with a novel posttranscriptional regulatory RNA element (PRE, which is located in the coding region of the CD83 transcript. Interestingly, this interaction commits the CD83 mRNA to efficient nuclear export via the CRM1 pathway. To date, however, the structural basis of this interaction, which potentially involves three distinct RNA recognition motifs (RRM1-3 in HuR and a complex three-pronged RNA stem-loop element in CD83 mRNA, has not been investigated in detail. In the present work we analyzed this interaction in vitro and in vivo using various HuR- and CD83 mRNA mutants. We are able to demonstrate that both, RRM1 and RRM2 are crucial for binding, whereas RRM3 as well as the HuR hinge region contributed only marginally to this protein:RNA interaction. Furthermore, mutation of uridine rich patches within the PRE did not disturb HuR:CD83 mRNA complex formation while, in contrast, the deletion of specific PRE subfragments from the CD83 mRNA prevented HuR binding in vitro and in vivo. Interestingly, the observed inhibition of HuR binding to CD83 mRNA does not lead to a nuclear trapping of the transcript but rather redirected this transcript from the CRM1- towards the NXF1/TAP-specific nuclear export pathway. Thus, the presence of a functional PRE permits nucleocytoplasmic trafficking of the CD83 transcript via the CRM1 pathway.

  8. Understanding Relations among Children's Shy and Antisocial/Aggressive Behaviors and Mothers' Parenting: The Role of Maternal Beliefs

    Science.gov (United States)

    Evans, Cortney A.; Nelson, Larry J.; Porter, Christin L.; Nelson, David A.; Hart, Craig H.

    2012-01-01

    This study assesses the relationships between children's shy and antisocial/aggressive behaviors and maternal beliefs, and concomitant parenting behaviors. Structural equation models examined 199 mothers' perceptions of aggression and shyness in their preschool-age children (average age = 59.63 months); maternal beliefs (i.e., locus of control,…

  9. A facility to search for hidden particles at the CERN SPS: the SHiP physics case

    Science.gov (United States)

    Alekhin, Sergey; Altmannshofer, Wolfgang; Asaka, Takehiko; Batell, Brian; Bezrukov, Fedor; Bondarenko, Kyrylo; Boyarsky, Alexey; Choi, Ki-Young; Corral, Cristóbal; Craig, Nathaniel; Curtin, David; Davidson, Sacha; de Gouvêa, André; Dell'Oro, Stefano; deNiverville, Patrick; Bhupal Dev, P. S.; Dreiner, Herbi; Drewes, Marco; Eijima, Shintaro; Essig, Rouven; Fradette, Anthony; Garbrecht, Björn; Gavela, Belen; Giudice, Gian F.; Goodsell, Mark D.; Gorbunov, Dmitry; Gori, Stefania; Grojean, Christophe; Guffanti, Alberto; Hambye, Thomas; Hansen, Steen H.; Helo, Juan Carlos; Hernandez, Pilar; Ibarra, Alejandro; Ivashko, Artem; Izaguirre, Eder; Jaeckel, Joerg; Jeong, Yu Seon; Kahlhoefer, Felix; Kahn, Yonatan; Katz, Andrey; Kim, Choong Sun; Kovalenko, Sergey; Krnjaic, Gordan; Lyubovitskij, Valery E.; Marcocci, Simone; Mccullough, Matthew; McKeen, David; Mitselmakher, Guenakh; Moch, Sven-Olaf; Mohapatra, Rabindra N.; Morrissey, David E.; Ovchynnikov, Maksym; Paschos, Emmanuel; Pilaftsis, Apostolos; Pospelov, Maxim; Hall Reno, Mary; Ringwald, Andreas; Ritz, Adam; Roszkowski, Leszek; Rubakov, Valery; Ruchayskiy, Oleg; Schienbein, Ingo; Schmeier, Daniel; Schmidt-Hoberg, Kai; Schwaller, Pedro; Senjanovic, Goran; Seto, Osamu; Shaposhnikov, Mikhail; Shchutska, Lesya; Shelton, Jessie; Shrock, Robert; Shuve, Brian; Spannowsky, Michael; Spray, Andy; Staub, Florian; Stolarski, Daniel; Strassler, Matt; Tello, Vladimir; Tramontano, Francesco; Tripathi, Anurag; Tulin, Sean; Vissani, Francesco; Winkler, Martin W.; Zurek, Kathryn M.

    2016-12-01

    This paper describes the physics case for a new fixed target facility at CERN SPS. The SHiP (search for hidden particles) experiment is intended to hunt for new physics in the largely unexplored domain of very weakly interacting particles with masses below the Fermi scale, inaccessible to the LHC experiments, and to study tau neutrino physics. The same proton beam setup can be used later to look for decays of tau-leptons with lepton flavour number non-conservation, τ \\to 3μ and to search for weakly-interacting sub-GeV dark matter candidates. We discuss the evidence for physics beyond the standard model and describe interactions between new particles and four different portals—scalars, vectors, fermions or axion-like particles. We discuss motivations for different models, manifesting themselves via these interactions, and how they can be probed with the SHiP experiment and present several case studies. The prospects to search for relatively light SUSY and composite particles at SHiP are also discussed. We demonstrate that the SHiP experiment has a unique potential to discover new physics and can directly probe a number of solutions of beyond the standard model puzzles, such as neutrino masses, baryon asymmetry of the Universe, dark matter, and inflation.

  10. Is Silence Golden? Elementary School Teachers' Strategies and Beliefs regarding Hypothetical Shy/Quiet and Exuberant/Talkative Children

    Science.gov (United States)

    Coplan, Robert J.; Hughes, Kathleen; Bosacki, Sandra; Rose-Krasnor, Linda

    2011-01-01

    The primary goal of the present study was to examine elementary teachers' strategies, attitudes, and beliefs regarding hypothetical shy (i.e., quiet), exuberant (i.e., overly talkative), and average (i.e., typical) children. We explored whether these strategies and beliefs varied as a function of the gender of the hypothetical child as well as…

  11. Hydroxyurea (HU) for prevention of recurrent stroke in sickle cell anemia (SCA).

    Science.gov (United States)

    Sumoza, Abraham; de Bisotti, Renate; Sumoza, David; Fairbanks, Virgil

    2002-11-01

    Cerebrovascular accident (CVA) is a major cause of morbidity and death in sickle cell anemia (SCA). Transfusion of packed erythrocytes is widely used to prevent this complication. However, chronic transfusion may lead to iron overload, alloimmunization, or infections. Cost and compliance may compromise transfusion therapy. A possible alternative, the prophylactic use of hydroxyurea (HU), has not been tried to determine whether it may prevent recurrent stroke. We used HU in five children with SCA who had suffered stroke, in three of them after a first episode and in the other two after a second CVA. Four had infarctive stroke and one a transient ischemic attack (TIA). Four patients took HU at a dose of 40 mg/kg/d, one patient at 30 mg/kg/d. None of the patients had recurrent stroke during 42-112 months of observation. None experienced pain crises. In all, HbF increased significantly and was maintained above 14.7% during treatment. The total Hb concentration increased 19.5 g/L (median) above the value before treatment. HU was well tolerated. None of the five children had leukopenia or thrombocytopenia during therapy. HU appears to prevent recurrence of stroke in SCA without risk of major toxicity.

  12. dNTP deficiency induced by HU via inhibiting ribonucleotide reductase affects neural tube development.

    Science.gov (United States)

    Guan, Zhen; Wang, Xiuwei; Dong, Yanting; Xu, Lin; Zhu, Zhiqiang; Wang, Jianhua; Zhang, Ting; Niu, Bo

    2015-02-03

    Exposure to environmental toxic chemicals in utero during the neural tube development period can cause developmental disorders. To evaluate the disruption of neural tube development programming, the murine neural tube defects (NTDs) model was induced by interrupting folate metabolism using methotrexate in our previous study. The present study aimed to examine the effects of dNTP deficiency induced by hydroxyurea (HU), a specific ribonucleotide reductase (RNR) inhibitor, during murine neural tube development. Pregnant C57BL/6J mice were intraperitoneally injected with various doses of HU on gestation day (GD) 7.5, and the embryos were checked on GD 11.5. RNR activity and deoxynucleoside triphosphate (dNTP) levels were measured in the optimal dose. Additionally, DNA damage was examined by comet analysis and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. Cellular behaviors in NTDs embryos were evaluated with phosphorylation of histone H3 (PH-3) and caspase-3 using immunohistochemistry and western blot analysis. The results showed that NTDs were observed mostly with HU treatment at an optimal dose of 225 mg/kg b/w. RNR activity was inhibited and dNTP levels were decreased in HU-treated embryos with NTDs. Additionally, increased DNA damage, decreased proliferation, and increased caspase-3 were significant in NTDs embryos compared to the controls. Results indicated that HU induced murine NTDs model by disturbing dNTP metabolism and further led to the abnormal cell balance between proliferation and apoptosis.

  13. Effect of swift heavy ion (SHI) irradiation on transparent conducting oxide electrodes for dye-sensitized solar cell applications

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Hemant Kr. [University School of Basic and Applied Sciences, Guru Gobind Singh Indraprastha University, New Delhi (India); Avasthi, D.K. [Inter University Accelerator Center, Post Box 10502, New Delhi (India); Aggarwal, Shruti, E-mail: shruti.al@gmail.com [University School of Basic and Applied Sciences, Guru Gobind Singh Indraprastha University, New Delhi (India)

    2015-06-15

    Highlights: •The objective is to study the effect of swift heavy ion (SHI) irradiation on photoanode of DSSC for better efficiency. •This work presents the effect of SHI irradiation on various Transparent conducting oxides (TCOs). •Effects are studied in terms of conductivity and transmittance of TCOs. •ITO-PET gives best results in comparison to ITO and FTO for DSSC application under SHI irradiation. -- Abstract: Transparent conducting oxides (TCOs) are used as electrodes in dye-sensitized solar cells (DSSCs) because of their properties such as high transmittance and low resistivity. In the present work, the effects of swift heavy ion (SHI) irradiation on various types of TCOs are presented. The objective of this study is to investigate the effect of SHI on TCOs. For the present study, three different types of TCOs are considered, namely, (a) FTO (fluorine-doped tin oxide, SnO{sub 2}:F) on a Nippon glass substrate, (b) ITO (indium tin oxide, In{sub 2}O{sub 3}:Sn) coated on polyethylene terephthalate (PET) on a Corning glass substrate, and (c) ITO on a Corning glass substrate. These films are irradiated with 120 MeV Ag{sup +9} ions at fluences ranging from 3.0 × 10{sup 11} ions/cm{sup 2} to 3.0 × 10{sup 13} ions/cm{sup 2}. The structural, morphological, optical and electrical properties are studied via X-ray diffraction (XRD), atomic force microscopy (AFM), UV–Vis absorption spectroscopy and four-probe resistivity measurements, respectively. The ITO-PET electrode is found to exhibit superior conductivity and transmittance properties in comparison with the others after irradiation and, therefore, to be the most suitable for solar cell applications.

  14. First reliability test of a surface micromachined microengine using SHiMMeR

    Energy Technology Data Exchange (ETDEWEB)

    Tanner, D.M.; Smith, N.F.; Bowman, D.J. [and others

    1997-08-01

    The first-ever reliability stress test on surface micromachined microengines developed at Sandia National Laboratories (SNL) has been completed. We stressed 41 microengines at 36,000 RPM and inspected the functionality at 60 RPM. We have observed an infant mortality region, a region of low failure rate (useful life), and no signs of wearout in the data. The reliability data are presented and interpreted using standard reliability methods. Failure analysis results on the stressed microengines are presented. In our effort to study the reliability of MEMS, we need to observe the failures of large numbers of parts to determine the failure modes. To facilitate testing of large numbers of micromachines. The Sandia High Volume Measurement of Micromachine Reliability (SHiMMeR) system has computer controlled positioning and the capability to inspect moving parts. The development of this parallel testing system is discussed in detail.

  15. Self-Identified Childhood Shyness and Perceptions of Shy Children: Voices of Elementary School Teachers

    Directory of Open Access Journals (Sweden)

    Mary Grace LAO

    2013-07-01

    Full Text Available Childhood shyness plays an important role in terms of learning and socialization and can have profound effects on individuals’ thoughts, feelings, and behaviors that can carry over into adulthood. The present study investigated how self-identified childhood shyness might influence elementary school teachers’ current perceptions of shyness among their students. Participants were 14 elementary school teachers. Interviews were conducted over the telephone and ranscribed for content analysis. Analysis revealed themes of social factors,personal factors,and relating to shyness that contributed to childhood shyness. Moreover, these experiences of childhood shyness appeared to contribute to teachers’ current perceptions of shyness (among teachers who self-identified as shy children. Implications for future research and holistic educational practices are discussed.

  16. SHI induced modification in structural, optical, dielectric and thermal properties of poly ethylene oxide films

    Science.gov (United States)

    Patel, Gnansagar B.; Bhavsar, Shilpa; Singh, N. L.; Singh, F.; Kulriya, P. K.

    2016-07-01

    Poly ethylene oxide (PEO) films were synthesized by solution cast method. These self-standing films were exposed with 60 MeV C+5 ion and 100 MeV Ni+7 ion at different fluences. SHI induced effect was investigated by employing various techniques. The crystalline size decreased upon irradiation as observed from XRD analysis. FTIR analysis reveals the decrement in the peak intensity upon irradiation. Tauc's method was used to determine the optical band gap (Eg), which shows decreasing trends with increase of fluence. The dielectric properties were investigated in the frequency range 10 Hz to 10 MHz for unirradiated and irradiated films. The dielectric constant remains same for the broad-spectrum of frequency and increases at lower frequency. The dielectric loss also moderately influence as a function of frequency due to irradiation. DSC analysis validated the results of XRD. Scanning electron microscopy (SEM) reveals that there is significant change in the surface morphology due to irradiation.

  17. Multiple Functions of the RNA-Binding Protein HuR in Cancer Progression, Treatment Responses and Prognosis

    Directory of Open Access Journals (Sweden)

    Baocheng Wang

    2013-05-01

    Full Text Available The human embryonic lethal abnormal vision-like protein, HuR, is a member of the Hu family of RNA-binding proteins. Over the past decade, this ubiquitously expressed protein has been extensively investigated in cancer research because it is involved in the regulation of mRNA stability and translation in many cell types. HuR activity and function is associated with its subcellular distribution, transcriptional regulation, translational and post-translational modifications. HuR regulation of target mRNAs is based on the interaction between the three specific domains of HuR protein and one or several U- or AU-rich elements (AREs in the untranslated region of target mRNAs. A number of cancer-related transcripts containing AREs, including mRNAs for proto-oncogenes, cytokines, growth factors, and invasion factors, have been characterized as HuR targets. It has been proposed that HuR has a central tumorigenic activity by enabling multiple cancer phenotypes. In this review, we comprehensively survey the existing evidence with regard to the diverse functions of HuR in caner development and progression. The current data also suggest that HuR might be a novel and promising therapeutic target and a marker for treatment response and prognostic evaluation.

  18. Temporally defined neocortical translation and polysome assembly are determined by the RNA-binding protein Hu antigen R.

    Science.gov (United States)

    Kraushar, Matthew L; Thompson, Kevin; Wijeratne, H R Sagara; Viljetic, Barbara; Sakers, Kristina; Marson, Justin W; Kontoyiannis, Dimitris L; Buyske, Steven; Hart, Ronald P; Rasin, Mladen-Roko

    2014-09-09

    Precise spatiotemporal control of mRNA translation machinery is essential to the development of highly complex systems like the neocortex. However, spatiotemporal regulation of translation machinery in the developing neocortex remains poorly understood. Here, we show that an RNA-binding protein, Hu antigen R (HuR), regulates both neocorticogenesis and specificity of neocortical translation machinery in a developmental stage-dependent manner in mice. Neocortical absence of HuR alters the phosphorylation states of initiation and elongation factors in the core translation machinery. In addition, HuR regulates the temporally specific positioning of functionally related mRNAs into the active translation sites, the polysomes. HuR also determines the specificity of neocortical polysomes by defining their combinatorial composition of ribosomal proteins and initiation and elongation factors. For some HuR-dependent proteins, the association with polysomes likewise depends on the eukaryotic initiation factor 2 alpha kinase 4, which associates with HuR in prenatal developing neocortices. Finally, we found that deletion of HuR before embryonic day 10 disrupts both neocortical lamination and formation of the main neocortical commissure, the corpus callosum. Our study identifies a crucial role for HuR in neocortical development as a translational gatekeeper for functionally related mRNA subgroups and polysomal protein specificity.

  19. Effects of Climate Change and Fisheries Bycatch on Shy Albatross (Thalassarche cauta) in Southern Australia.

    Science.gov (United States)

    Thomson, Robin B; Alderman, Rachael L; Tuck, Geoffrey N; Hobday, Alistair J

    2015-01-01

    The impacts of climate change on marine species are often compounded by other stressors that make direct attribution and prediction difficult. Shy albatrosses (Thalassarche cauta) breeding on Albatross Island, Tasmania, show an unusually restricted foraging range, allowing easier discrimination between the influence of non-climate stressors (fisheries bycatch) and environmental variation. Local environmental conditions (rainfall, air temperature, and sea-surface height, an indicator of upwelling) during the vulnerable chick-rearing stage, have been correlated with breeding success of shy albatrosses. We use an age-, stage- and sex-structured population model to explore potential relationships between local environmental factors and albatross breeding success while accounting for fisheries bycatch by trawl and longline fisheries. The model uses time-series of observed breeding population counts, breeding success, adult and juvenile survival rates and a bycatch mortality observation for trawl fishing to estimate fisheries catchability, environmental influence, natural mortality rate, density dependence, and productivity. Observed at-sea distributions for adult and juvenile birds were coupled with reported fishing effort to estimate vulnerability to incidental bycatch. The inclusion of rainfall, temperature and sea-surface height as explanatory variables for annual chick mortality rate was statistically significant. Global climate models predict little change in future local average rainfall, however, increases are forecast in both temperatures and upwelling, which are predicted to have detrimental and beneficial effects, respectively, on breeding success. The model shows that mitigation of at least 50% of present bycatch is required to offset losses due to future temperature changes, even if upwelling increases substantially. Our results highlight the benefits of using an integrated modeling approach, which uses available demographic as well as environmental data

  20. Effects of Climate Change and Fisheries Bycatch on Shy Albatross (Thalassarche cauta in Southern Australia.

    Directory of Open Access Journals (Sweden)

    Robin B Thomson

    Full Text Available The impacts of climate change on marine species are often compounded by other stressors that make direct attribution and prediction difficult. Shy albatrosses (Thalassarche cauta breeding on Albatross Island, Tasmania, show an unusually restricted foraging range, allowing easier discrimination between the influence of non-climate stressors (fisheries bycatch and environmental variation. Local environmental conditions (rainfall, air temperature, and sea-surface height, an indicator of upwelling during the vulnerable chick-rearing stage, have been correlated with breeding success of shy albatrosses. We use an age-, stage- and sex-structured population model to explore potential relationships between local environmental factors and albatross breeding success while accounting for fisheries bycatch by trawl and longline fisheries. The model uses time-series of observed breeding population counts, breeding success, adult and juvenile survival rates and a bycatch mortality observation for trawl fishing to estimate fisheries catchability, environmental influence, natural mortality rate, density dependence, and productivity. Observed at-sea distributions for adult and juvenile birds were coupled with reported fishing effort to estimate vulnerability to incidental bycatch. The inclusion of rainfall, temperature and sea-surface height as explanatory variables for annual chick mortality rate was statistically significant. Global climate models predict little change in future local average rainfall, however, increases are forecast in both temperatures and upwelling, which are predicted to have detrimental and beneficial effects, respectively, on breeding success. The model shows that mitigation of at least 50% of present bycatch is required to offset losses due to future temperature changes, even if upwelling increases substantially. Our results highlight the benefits of using an integrated modeling approach, which uses available demographic as well as

  1. Coordination of genomic structure and transcription by the main bacterial nucleoid-associated protein HU.

    Science.gov (United States)

    Berger, Michael; Farcas, Anca; Geertz, Marcel; Zhelyazkova, Petya; Brix, Klaudia; Travers, Andrew; Muskhelishvili, Georgi

    2010-01-01

    The histone-like protein HU is a highly abundant DNA architectural protein that is involved in compacting the DNA of the bacterial nucleoid and in regulating the main DNA transactions, including gene transcription. However, the coordination of the genomic structure and function by HU is poorly understood. Here, we address this question by comparing transcript patterns and spatial distributions of RNA polymerase in Escherichia coli wild-type and hupA/B mutant cells. We demonstrate that, in mutant cells, upregulated genes are preferentially clustered in a large chromosomal domain comprising the ribosomal RNA operons organized on both sides of OriC. Furthermore, we show that, in parallel to this transcription asymmetry, mutant cells are also impaired in forming the transcription foci-spatially confined aggregations of RNA polymerase molecules transcribing strong ribosomal RNA operons. Our data thus implicate HU in coordinating the global genomic structure and function by regulating the spatial distribution of RNA polymerase in the nucleoid.

  2. Ship Targets Discrimination Algorithm in SAR Images Based on Hu Moment Feature and Texture Feature

    Directory of Open Access Journals (Sweden)

    Liu Lei

    2016-01-01

    Full Text Available To discriminate the ship targets in SAR images, this paper proposed the method based on combination of Hu moment feature and texture feature. Firstly,7 Hu moment features should be extracted, while gray level co-occurrence matrix is then used to extract the features of mean, variance, uniformity, energy, entropy, inertia moment, correlation and differences. Finally the k-neighbour classifier was used to analysis the 15 dimensional feature vectors. The experimental results show that the method of this paper has a good effect.

  3. Neuroprotective effects of the cannabinoid agonist HU210 on retinal degeneration

    OpenAIRE

    Lax Zapata, Pedro; Esquiva Sobrino, Gema; Altavilla, Cesare; Cuenca Navarro, Nicolás

    2014-01-01

    Cannabinoids have been demonstrated to exert neuroprotective effects on different types of neuronal insults. Here we have addressed the therapeutic potential of the synthetic cannabinoid HU210 on photoreceptor degeneration, synaptic connectivity and functional activity of the retina in the transgenic P23H rat, an animal model for autosomal dominant retinitis pigmentosa (RP). In P23H rats administered with HU210 (100 μg/kg, i.p.) from P24 to P90, ERG recordings showed an amelioration of vision...

  4. The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA.

    Science.gov (United States)

    López de Silanes, Isabel; Gorospe, Myriam; Taniguchi, Hiroaki; Abdelmohsen, Kotb; Srikantan, Subramanya; Alaminos, Miguel; Berdasco, María; Urdinguio, Rocío G; Fraga, Mario F; Jacinto, Filipe V; Esteller, Manel

    2009-05-01

    The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression levels increase during tumorigenesis. Here, we present evidence that the expression of DNMT3b is post-transcriptionally regulated by HuR, an RNA-binding protein that stabilizes and/or modulates the translation of target mRNAs. The presence of a putative HuR-recognition motif in the DNMT3b 3'UTR prompted studies to investigate if this transcript associated with HuR. The interaction between HuR and DNMT3b mRNA was studied by immunoprecipitation of endogenous HuR ribonucleoprotein complexes followed by RT-qPCR detection of DNMT3b mRNA, and by in vitro pulldown of biotinylated DNMT3b RNAs followed by western blotting detection of HuR. These studies revealed that binding of HuR stabilized the DNMT3b mRNA and increased DNMT3b expression. Unexpectedly, cisplatin treatment triggered the dissociation of the [HuR-DNMT3b mRNA] complex, in turn promoting DNMT3b mRNA decay, decreasing DNMT3b abundance, and lowering the methylation of repeated sequences and global DNA methylation. In summary, our data identify DNMT3b mRNA as a novel HuR target, present evidence that HuR affects DNMT3b expression levels post-transcriptionally, and reveal the functional consequences of the HuR-regulated DNMT3b upon DNA methylation patterns.

  5. 新文化运动时期胡适女性主义思想研究综述%On HU Shi's Feminist Thought during the New Culture Movement

    Institute of Scientific and Technical Information of China (English)

    叶长青

    2009-01-01

    新文化运动时期,胡适提出了自己的女性主义观念.20世纪80年代后,随着大陆胡适研究的兴起,胡适的女性主义思想也逐渐引起学术界的关注,并出现了与之相关的一些著述,这一课题已成为热点问题.

  6. Review: Hu Houxuan, "Xia and the Tu State in the Oracle Inscriptions: A Consideration" Gudai Chengbang Shi Yanjiu (Studies on Ancient City States) Renmin Press, 1989, Pp. 340-353.

    Institute of Scientific and Technical Information of China (English)

    RiZhi

    1995-01-01

    This article verified that the Tu state mentioned in the oracle inscriptions was the Xia people after the Xia Dynasty had been conquered by Tang, the first king of the Shang Dynasty m c.a. 16th century B.C. The state of Xia, which originated from Yu, was entitled "Xia Hou"; its king was hence called "Xia Hou Di" (Emperor of Xia Hou), e.g."Xia Hou Di Qi". The Xia people, together with the Yin (Shang) people, and the Zhou people, were the so-called "people of San Dai" (Three Dynasties). As mentioned in "Lunyu, Bayi", they founded the states, with the Xia people being associated with the pine, the Yin people with the cypress, and the Zhou people with the chestnut.

  7. Hu Shi, Lei Zhen and "Free China": Concurrent Discussion on the Path for Realizing Freedom of Speech%胡适与雷震及《自由中国》——与马庆先生商榷

    Institute of Scientific and Technical Information of China (English)

    吴麟

    2010-01-01

    在创办时,雷震与胡适的基本理念并不迥异,只是具体观点有别.胡适在"雷震案"中的表现常遭误解,其实他已尽力斡旋.胡适支持积极争取言论自由,同时再三告诫:富有勇气之外还应讲究技巧才能在威权政治体制下延续与拓展生存空间.

  8. 胡适《三国演义》研究的贡献与偏颇%Contributions and Biases of Hu Shi's Study on Romance of the Three Kingdoms

    Institute of Scientific and Technical Information of China (English)

    汪大白

    2011-01-01

    对于研究来说,胡适的考证及其结论具有奠基性意义,甚至他认为"是一部很有势力的通俗历史讲义"的观点对于突破今天狭隘的文学研究也有某种启示.然而,胡适否定的文学价值,其学术见解与价值评判也存在着严重偏颇.

  9. Generation and Interpretation of Hu Shi's Experimenter Identity of New Poetry%胡适新诗“尝试者”身份的生成及其解读史

    Institute of Scientific and Technical Information of China (English)

    黄晓东

    2015-01-01

    胡适对新诗的“尝试”能够取得成功有三个原因:一是外部条件的变化;二是文言不能适应文学在新形势下的需要;三是胡适在与梅光迪等人进行“文白之辩”时受到的启发.而此后的新诗教育中对其“尝试者”身份的解读也有很大不同:民国时期废名、苏雪林、朱自清等人的颇具学理性;“十七年”时期则偏向于“政治性”;而新时期以来则又重回学理性与客观性.

  10. The HU Regulon Is Composed of Genes Responding to Anaerobiosis, Acid Stress, High Osmolarity and SOS Induction

    Science.gov (United States)

    Oberto, Jacques; Nabti, Sabrina; Jooste, Valérie; Mignot, Hervé; Rouviere-Yaniv, Josette

    2009-01-01

    Background The Escherichia coli heterodimeric HU protein is a small DNA-bending protein associated with the bacterial nucleoid. It can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Cells lacking HU grow very poorly and display many phenotypes. Methodology/Principal Findings We analyzed the transcription profile of every Escherichia coli gene in the absence of one or both HU subunits. This genome-wide in silico transcriptomic approach, performed in parallel with in vivo genetic experimentation, defined the HU regulon. This large regulon, which comprises 8% of the genome, is composed of four biologically relevant gene classes whose regulation responds to anaerobiosis, acid stress, high osmolarity, and SOS induction. Conclusions/Significance The regulation a large number of genes encoding enzymes involved in energy metabolism and catabolism pathways by HU explains the highly pleiotropic phenotype of HU-deficient cells. The uniform chromosomal distribution of the many operons regulated by HU strongly suggests that the transcriptional and nucleoid architectural functions of HU constitute two aspects of a unique protein-DNA interaction mechanism. PMID:19194530

  11. The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction.

    Directory of Open Access Journals (Sweden)

    Jacques Oberto

    Full Text Available BACKGROUND: The Escherichia coli heterodimeric HU protein is a small DNA-bending protein associated with the bacterial nucleoid. It can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Cells lacking HU grow very poorly and display many phenotypes. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed the transcription profile of every Escherichia coli gene in the absence of one or both HU subunits. This genome-wide in silico transcriptomic approach, performed in parallel with in vivo genetic experimentation, defined the HU regulon. This large regulon, which comprises 8% of the genome, is composed of four biologically relevant gene classes whose regulation responds to anaerobiosis, acid stress, high osmolarity, and SOS induction. CONCLUSIONS/SIGNIFICANCE: The regulation a large number of genes encoding enzymes involved in energy metabolism and catabolism pathways by HU explains the highly pleiotropic phenotype of HU-deficient cells. The uniform chromosomal distribution of the many operons regulated by HU strongly suggests that the transcriptional and nucleoid architectural functions of HU constitute two aspects of a unique protein-DNA interaction mechanism.

  12. Immunostimulatory effects of natural human interferon-alpha (huIFN-alpha) on carps Cyprinus carpio L.

    Science.gov (United States)

    Watanuki, Hironobu; Chakraborty, Gunimala; Korenaga, Hiroki; Kono, Tomoya; Shivappa, R B; Sakai, Masahiro

    2009-10-15

    Human interferon-alpha (huIFN-alpha) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. However, the immunostimulatory effects of huIFN-alpha in fish remain to be investigated. In the current study, the immune responses of the carp species Cyprinus carpio L. to treatment with huIFN-alpha were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes including interleukin-1beta, tumor necrosis factor-alpha and interleukin 10. Low doses of huIFN-alpha were administered orally once a day for 3 days, and sampling was carried out at 1, 3 and 5 days post-treatment. Our results indicate that a low dose of huIFN-alpha significantly increased phagocytic activity and superoxide anion production in the carp kidney. The huIFN-alpha-treated fish also displayed a significant upregulation in cytokine gene expression. The current study demonstrates the stimulatory effects of huIFN-alpha on the carp immune system and highlights the immunomodulatory role of huIFN-alpha in fish.

  13. First International Workshop on Human Factors in Modeling (HuFaMo 2015)

    DEFF Research Database (Denmark)

    Störrle, Harald; Chaudron, Michel R. V.; Amaral, Vasco

    2015-01-01

    and systematic approaches to assert usability in modeling and confirm the claims of productivity. This workshop creates a space for discussion being a get together of both MDE, Usability, Human Interfaces and the Experimental Software engineering community. HuFaMo expressly focuses on human factors, in order...

  14. Rare Association of Anti-Hu Antibody Positive Paraneoplastic Neurological Syndrome and Transitional Cell Bladder Carcinoma

    Directory of Open Access Journals (Sweden)

    S. Lukacs

    2012-01-01

    Full Text Available Introduction. Paraneoplastic encephalomyelitis (PEM and subacute sensory neuronopathy (SSN are remote effects of cancer, usually associated with small-cell lung carcinoma and positive anti-Hu antibody. We describe the rare association of bladder transitional cell carcinoma (TCC with anti-Hu antibody positivity resulting in this paraneoplastic neurological syndrome. Patient. A 76-year-old female presented with bilateral muscle weakness and paraesthesia of the upper and lower limbs in a length-dependent “glove and stocking” distribution. Central nervous system symptoms included cognitive problems, personality change, and truncal ataxia. Case notes and the literature were reviewed. Result. Autoantibody screening was positive for anti-Hu antibody (recently renamed antineuronal nuclear antibody 1, ANNA-1. The diagnosis of PEM and SSN was supported by MRI and lumbar puncture results. A superficial bladder TCC was demonstrated on CT and subsequently confirmed on histology. No other primary neoplasm was found on full-body imaging. The neurological symptoms were considered to be an antibody-mediated paraneoplastic neurological syndrome and improved after resection of the tumour. Discussion. The association of anti-Hu positive paraneoplastic neurological syndrome and TCC has not been described in the literature previously. We emphasize the need for detailed clinical examination and the importance of a multidisciplinary thought process and encourage further awareness of this rare association.

  15. DAR2 acts as an important node connecting cytokinin, auxin, SHY2 and PLT1/2 in root meristem size control.

    Science.gov (United States)

    Peng, Yuancheng; Chen, Liangliang; Lu, Yaru; Ma, Wenying; Tong, Yiping; Li, Yunhai

    2013-06-01

    Cytokinin and auxin antagonistically affect cell proliferation and differentiation and thus regulate root meristem size by influencing the abundance of SHORT HYPOCOTYL2 (SHY2/IAA3). SHY2 affects auxin distribution in the root meristem by repressing the auxin-inducible expression of PIN-FORMED (PIN) auxin transport genes. The PLETHORA (PLT1/2) genes influence root meristem growth by promoting stem cells and transit-amplifying cells. However, the factors connecting cytokinin, auxin, SHY2 and PLT1/2 are largely unknown. In a recent study, we have shown that the DA1-related protein 2 (DAR2) acts downstream of cytokinin and SHY2 but upstream of PLT1/2 to affect root meristem size. Here, we discuss the possible molecular mechanisms by which Arabidopsis DAR2 controls root meristem size.

  16. HuB localizes to polysomes and alters C/EBP-beta expression in 3T3-L1 adipocytes.

    Science.gov (United States)

    Gantt, Kira R; Jain, Renu G; Dudek, Ronald W; Pekala, Phillip H

    2004-01-16

    The RNA binding protein HuB was ectopically expressed in 3T3-L1 preadipocytes and localized primarily to the nucleus, as the cells differentiated HuB redistributed to the cytosol and on analysis localized to the dense polysomes. Electron micrographs confirm association of HuB with the ribosomes in the adipocytes consistent with a proposed role in control of translation and mRNA stability. Examination of the expression of C/EBP-beta in the cells expressing HuB relative to the parental 3T3-L1 adipocytes demonstrated an alteration in the LAP to LIP ratio. The data support a role for endogenous Hu proteins in the differentiation process, potentially affecting the rate of differentiation by controlling the concentration of the dominant negative transcription inhibitor, LIP.

  17. Redox behaviors of the neurotoxic portion in human prion protein, HuPrP(106-126)

    Science.gov (United States)

    Yamamoto, Norifumi; Kuwata, Kazuo

    2010-09-01

    A peptide fragment of human prion protein, HuPrP(106-126), has been reported to mimic the pathological features underlying prion diseases. Although the actual neurotoxic mechanism of HuPrP(106-126) has not been elucidated, several hypotheses has been proposed based on the role for copper. In this study, to understand the toxic function of HuPrP(106-126) from a viewpoint of electrochemical competence, we investigated redox properties of copper ion complexes with four different binding motifs of a model of HuPrP(106-126) based on density functional theory calculations. We found that the HuPrP(106-126)-derived models exhibited diverse redox activities that depended on copper-binding conformations.

  18. 浅谈尿路感染从湿热论治%Treating urinary tract infection from Shi're

    Institute of Scientific and Technical Information of China (English)

    李智全; 张靖芳; 卢跃卿

    2015-01-01

    Urinary tract infections are common urinary system diseases, belongs to the Lin syndrome. The important pathogenesis of urinary tract infection is Shi're. From TCM theory, discussion on the important role of Shi're in the urinary tract infection was significant.%尿路感染是常见的泌尿系统疾病,属中医"淋证"范畴.湿热是尿路感染迁延难愈和持续发展的重要病因病机,从中医理论出发探讨湿热在尿路感染中的重要作用,对临床更具指导意义.

  19. Shi Tiesheng's Religious Outlook%史铁生的宗教观

    Institute of Scientific and Technical Information of China (English)

    胡山林

    2011-01-01

    Due to the unlucky fate,Shi Tie sheng read some religious books for the sake of ideological liberation,and constantly talked about religious beliefs and Buddha sayings.During this period,he had his own unique understanding of religion through his own experience,forming a particular spiritual personality of religion.The main content is that the Buddha is not a source of blessing but a rescue road for the souls in misery;People can head for the heaven,but never get to it,as the heaven always exists in the process of pursuing life belief,Religious spirit is not equal to religion and religion resorts to the salvation of human beings by God.Religious spirit is that people save themselves with their own spiritual power.The essense of Shi Tiesheng's religious outlook is seeking for a final home of soul both for himself and the human race.%由于命运的不幸,史铁生为求思想上的解脱看了一些宗教类的书,也曾不断地谈佛说道谈信仰谈宗教。这期间,史铁生结合自己的体验对宗教有了自己独特的理解,形成了独具精神个性的宗教观。主要内容是:佛(神)不是福报的源泉而是苦难里心魂的一条救路;人可以走向天堂,不可以走到天堂,天堂在人追求信仰的过程中;宗教精神不等于宗教,宗教的要旨是靠神对人的拯救,宗教精神是人依靠自己的精神力量对自我的拯救。史铁生宗教观的实质是为自己为人类寻求灵魂的归宿。

  20. Drug Transporter Expression and Activity in Human Hepatoma HuH-7 Cells

    Directory of Open Access Journals (Sweden)

    Elodie Jouan

    2016-12-01

    Full Text Available Human hepatoma cells may represent a valuable alternative to the use of human hepatocytes for studying hepatic drug transporters, which is now a regulatory issue during drug development. In the present work, we have characterized hepatic drug transporter expression, activity and regulation in human hepatoma HuH-7 cells, in order to determine the potential relevance of these cells for drug transport assays. HuH-7 cells displayed notable multidrug resistance-associated protein (MRP activity, presumed to reflect expression of various hepatic MRPs, including MRP2. By contrast, they failed to display functional activities of the uptake transporters sodium taurocholate co-transporting polypeptide (NTCP, organic anion-transporting polypeptides (OATPs and organic cation transporter 1 (OCT1, and of the canalicular transporters P-glycoprotein and breast cancer resistance protein (BCRP. Concomitantly, mRNA expressions of various sinusoidal and canalicular hepatic drug transporters were not detected (NTCP, OATP1B1, organic anion transporter 2 (OAT2, OCT1 and bile salt export pump or were found to be lower (OATP1B3, OATP2B1, multidrug and toxin extrusion protein 1, BCRP and MRP3 in hepatoma HuH-7 cells than those found in human hepatocytes, whereas other transporters such as OAT7, MRP4 and MRP5 were up-regulated. HuH-7 cells additionally exhibited farnesoid X receptor (FXR- and nuclear factor erythroid 2-related factor 2 (Nrf2-related up-regulation of some transporters. Such data indicate that HuH-7 cells, although expressing rather poorly some main hepatic drug transporters, may be useful for investigating interactions of drugs with MRPs, notably MRP2, and for studying FXR- or Nrf2-mediated gene regulation.

  1. Discriminating between HuR and TTP binding sites using the k-spectrum kernel method

    Science.gov (United States)

    Goldberg, Debra S.; Dowell, Robin

    2017-01-01

    Background The RNA binding proteins (RBPs) human antigen R (HuR) and Tristetraprolin (TTP) are known to exhibit competitive binding but have opposing effects on the bound messenger RNA (mRNA). How cells discriminate between the two proteins is an interesting problem. Machine learning approaches, such as support vector machines (SVMs), may be useful in the identification of discriminative features. However, this method has yet to be applied to studies of RNA binding protein motifs. Results Applying the k-spectrum kernel to a support vector machine (SVM), we first verified the published binding sites of both HuR and TTP. Additional feature engineering highlighted the U-rich binding preference of HuR and AU-rich binding preference for TTP. Domain adaptation along with multi-task learning was used to predict the common binding sites. Conclusion The distinction between HuR and TTP binding appears to be subtle content features. HuR prefers strongly U-rich sequences whereas TTP prefers AU-rich as with increasing A content, the sequences are more likely to be bound only by TTP. Our model is consistent with competitive binding of the two proteins, particularly at intermediate AU-balanced sequences. This suggests that fine changes in the A/U balance within a untranslated region (UTR) can alter the binding and subsequent stability of the message. Both feature engineering and domain adaptation emphasized the extent to which these proteins recognize similar general sequence features. This work suggests that the k-spectrum kernel method could be useful when studying RNA binding proteins and domain adaptation techniques such as feature augmentation could be employed particularly when examining RBPs with similar binding preferences. PMID:28333956

  2. Transcriptomic-Wide Discovery of Direct and Indirect HuR RNA Targets in Activated CD4+ T Cells.

    Directory of Open Access Journals (Sweden)

    Patsharaporn Techasintana

    Full Text Available Due to poor correlation between steady state mRNA levels and protein product, purely transcriptomic profiling methods may miss genes posttranscriptionally regulated by RNA binding proteins (RBPs and microRNAs (miRNAs. RNA immunoprecipitation (RIP methods developed to identify in vivo targets of RBPs have greatly elucidated those mRNAs which may be regulated via transcript stability and translation. The RBP HuR (ELAVL1 and family members are major stabilizers of mRNA. Many labs have identified HuR mRNA targets; however, many of these analyses have been performed in cell lines and oftentimes are not independent biological replicates. Little is known about how HuR target mRNAs behave in conditional knock-out models. In the present work, we performed HuR RIP-Seq and RNA-Seq to investigate HuR direct and indirect targets using a novel conditional knock-out model of HuR genetic ablation during CD4+ T activation and Th2 differentiation. Using independent biological replicates, we generated a high coverage RIP-Seq data set (>160 million reads that was analyzed using bioinformatics methods specifically designed to find direct mRNA targets in RIP-Seq data. Simultaneously, another set of independent biological replicates were sequenced by RNA-Seq (>425 million reads to identify indirect HuR targets. These direct and indirect targets were combined to determine canonical pathways in CD4+ T cell activation and differentiation for which HuR plays an important role. We show that HuR may regulate genes in multiple canonical pathways involved in T cell activation especially the CD28 family signaling pathway. These data provide insights into potential HuR-regulated genes during T cell activation and immune mechanisms.

  3. Intestinal epithelial HuR modulates distinct pathways of proliferation and apoptosis and attenuates small intestinal and colonic tumor development.

    Science.gov (United States)

    Giammanco, Antonina; Blanc, Valerie; Montenegro, Grace; Klos, Coen; Xie, Yan; Kennedy, Susan; Luo, Jianyang; Chang, Sung-Hee; Hla, Timothy; Nalbantoglu, Ilke; Dharmarajan, Sekhar; Davidson, Nicholas O

    2014-09-15

    HuR is a ubiquitous nucleocytoplasmic RNA-binding protein that exerts pleiotropic effects on cell growth and tumorigenesis. In this study, we explored the impact of conditional, tissue-specific genetic deletion of HuR on intestinal growth and tumorigenesis in mice. Mice lacking intestinal expression of HuR (Hur (IKO) mice) displayed reduced levels of cell proliferation in the small intestine and increased sensitivity to doxorubicin-induced acute intestinal injury, as evidenced by decreased villus height and a compensatory shift in proliferating cells. In the context of Apc(min/+) mice, a transgenic model of intestinal tumorigenesis, intestinal deletion of the HuR gene caused a three-fold decrease in tumor burden characterized by reduced proliferation, increased apoptosis, and decreased expression of transcripts encoding antiapoptotic HuR target RNAs. Similarly, Hur(IKO) mice subjected to an inflammatory colon carcinogenesis protocol [azoxymethane and dextran sodium sulfate (AOM-DSS) administration] exhibited a two-fold decrease in tumor burden. Hur(IKO) mice showed no change in ileal Asbt expression, fecal bile acid excretion, or enterohepatic pool size that might explain the phenotype. Moreover, none of the HuR targets identified in Apc(min/+)Hur(IKO) were altered in AOM-DSS-treated Hur(IKO) mice, the latter of which exhibited increased apoptosis of colonic epithelial cells, where elevation of a unique set of HuR-targeted proapoptotic factors was documented. Taken together, our results promote the concept of epithelial HuR as a contextual modifier of proapoptotic gene expression in intestinal cancers, acting independently of bile acid metabolism to promote cancer. In the small intestine, epithelial HuR promotes expression of prosurvival transcripts that support Wnt-dependent tumorigenesis, whereas in the large intestine epithelial HuR indirectly downregulates certain proapoptotic RNAs to attenuate colitis-associated cancer. Cancer Res; 74(18); 5322-35. ©2014 AACR.

  4. Two Choices: Li Dazhao and Hu Shi during the May Fourth Movement: An Investigation of the Relations between Li Dazhao and Hu Shi in Their Dispute over "Problems and - isms"%两种选择:"五四"时期的李大钊与胡适——"问题与主义"之争中李大钊与胡适关系探析

    Institute of Scientific and Technical Information of China (English)

    刘志光

    2007-01-01

    "问题与主义"之争涉及马克思主义与实验主义之间的最初冲突.虽然李大钊与胡适在论战中各自表达了不同的哲学与社会政治思想,但是这种冲突具有"原初"性质;在李大钊的"根本解决"与胡适的"点滴改良"之间存在着可以沟通的环节;李大钊与胡适的关系表明,中国革命可以在原则的坚定性和策略的灵活性之间保持"必要的张力".

  5. A facility to Search for Hidden Particles at the CERN SPS: the SHiP physics case

    CERN Document Server

    Alekhin, Sergey; Asaka, Takehiko; Batell, Brian; Bezrukov, Fedor; Bondarenko, Kyrylo; Boyarsky, Alexey; Choi, Ki-Young; Corral, Cristobal; Craig, Nathaniel; Curtin, David; Davidson, Sacha; de Gouvea, Andre; Dell'Oro, Stefano; deNiverville, Patrick; Bhupal Dev, P.S.; Dreiner, Herbi; Drewes, Marco; Eijima, Shintaro; Essig, Rouven; Fradette, Anthony; Garbrecht, Bjorn; Gavela, Belen; Giudice, Gian F.; Goodsell, Mark D.; Gorbunov, Dmitry; Gori, Stefania; Grojean, Christophe; Guffanti, Alberto; Hambye, Thomas; Hansen, Steen H.; Helo, Juan Carlos; Hernandez, Pilar; Ibarra, Alejandro; Ivashko, Artem; Izaguirre, Eder; Jaeckel, Joerg; Jeong, Yu Seon; Kahlhoefer, Felix; Kahn, Yonatan; Katz, Andrey; Kim, Choong Sun; Kovalenko, Sergey; Krnjaic, Gordan; Lyubovitskij, Valery E.; Marcocci, Simone; Mccullough, Matthew; McKeen, David; Mitselmakher, Guenakh; Moch, Sven-Olaf; Mohapatra, Rabindra N.; Morrissey, David E.; Ovchynnikov, Maksym; Paschos, Emmanuel; Pilaftsis, Apostolos; Pospelov, Maxim; Reno, Mary Hall; Ringwald, Andreas; Ritz, Adam; Roszkowski, Leszek; Rubakov, Valery; Ruchayskiy, Oleg; Schienbein, Ingo; Schmeier, Daniel; Schmidt-Hoberg, Kai; Schwaller, Pedro; Senjanovic, Goran; Seto, Osamu; Shaposhnikov, Mikhail; Shchutska, Lesya; Shelton, Jessie; Shrock, Robert; Shuve, Brian; Spannowsky, Michael; Spray, Andy; Staub, Florian; Stolarski, Daniel; Strassler, Matt; Tello, Vladimir; Tramontano, Francesco; Tripathi, Anurag; Tulin, Sean; Vissani, Francesco; Winkler, Martin W.; Zurek, Kathryn M.; CERN. Geneva. SPS and PS Experiments Committee; SPSC

    2016-01-01

    This paper describes the physics case for a new fixed target facility at CERN SPS. The SHiP (Search for Hidden Particles) experiment is intended to hunt for new physics in the largely unexplored domain of very weakly interacting particles with masses below the Fermi scale, inaccessible to the LHC experiments, and to study tau neutrino physics. The same proton beam setup can be used later to look for decays of tau-leptons with lepton flavour number non-conservation, $\\tau\\to 3\\mu$ and to search for weakly-interacting sub-GeV dark matter candidates. We discuss the evidence for physics beyond the Standard Model and describe interactions between new particles and four different portals - scalars, vectors, fermions or axion-like particles. We discuss motivations for different models, manifesting themselves via these interactions, and how they can be probed with the SHiP experiment and present several case studies. The prospects to search for relatively light SUSY and composite particles at SHiP are also discussed....

  6. Cosmic-ray exposure histories of the lunar meteorites AaU 012 and Shişr 166

    Science.gov (United States)

    Mészáros, Marianna; Leya, Ingo; Hofmann, Beda A.

    2017-09-01

    We measured the concentrations and isotopic compositions of the stable isotopes of He, Ne, Ar, Kr, and Xe in the two lunar impact-melt breccias Abar al' Uj (AaU) 012 and Shişr 166 to obtain information on their cosmic-ray exposure histories and possible launch pairing; the latter was suggested because of their similar chemical composition. AaU 012 has higher gas concentrations than Shişr 166 and clearly contains implanted solar wind gases, indicating a shallow to moderate shielding for this meteorite in the lunar regolith. The maximum shielding depth of AaU 012 was most likely ≤310 g cm-2 and its lunar regolith residence time was ≥420 ± 70 Ma. Our results indicate that in Shişr 166 the trapped component is a mixture of air and solar wind. The low concentration of cosmogenic and solar wind gases indicate substantial diffusive gas loss and a shielding depth of exposure history on the Moon does not exclude the possibility that the two meteorites were ejected by a single, large impact event.

  7. Cognitive Research on Lüshi and Antithesis%律诗及其对仗的认知探究

    Institute of Scientific and Technical Information of China (English)

    张炼强

    2015-01-01

    律诗及其对仗,历来为旧体诗研究者所关注. 不过,从认知角度来研究的,似不曾见到.文章作一个尝试.基于语言与认知的关系,我们看到律诗及其对仗,都是以整齐对称和错落参差以及"起点—路经—终点"认知图式为认知底蕴的.而这些认知底蕴可以从律诗及其对仗的语言的意义内容与语言的形式两个方面看到.这是文章探究的出发点,也是文章探究的归宿.%Researchers for classical poetry always focus on Lüshi and its antithesis. However, it seems not to have seen from a cognitive point of view. The article makes a try. Based on the relationship between language and cognition, we see Lüshi and its antithesis as symmetry and random irregularity as well as cognitive schema of "starting point—path—terminal point" as cognitive foundation. These details can be seen from the cognitive meaning of content and language form of Lüshi and its antithesis. This is the starting point and the end result of the article.

  8. Texture research on the circulating editions and it's composition of Dou tai shi zhen jing(the acu-puncture canon of Dou tai shi)%《窦太师针经》传本及构成考

    Institute of Scientific and Technical Information of China (English)

    黄幼民; 黄龙祥

    2009-01-01

    为元代针灸古籍,现存清代及民国两种抄本.清代抄本题作"秘抄杨氏家传针经图",民国抄本与"玉龙歌"合抄,题作"玉龙歌".经考证,这两种抄本确系的不同传本,但确切书名尚无定论.其作者并非窦汉卿本人,当是窦氏传人整理,又经后人补注.其中清代抄本具有本文、附注、提行集注、眉批等多种构成.由于为甄权后又一次,也是唯一次对针灸腧穴基于临床实践的总结,因而极具文献价值和实用价值.%Dou tai shi zhen jing(the acupuncture canon of Dou tai shi)was a ancient book of acupuncture and moxibustion of Yuan dynasty which had two kinds of transcript of Qing dynasty and the Republic of China.The transcript of qing dynasty entitled"Mi chao yang shi jia chuan zhen jing tu"(The secret note of acupuncture canon picture of Yang's family heritage).The transcript of the Republic of China was copied together with Yu long ge,so entitled Yu long ge.By making texture research,the two kinds of transcripts were actually different circulating editions of Dou tai shi zhen jing,but the exact title Was still uncer-tain.The author of this book Was not DOU Han-qing himself,It should be arranged by Dou's successor and annotated by his descendants.The transcript of Qing dynasty consisted of text,annotation,variorum and headnote.Because Dou tai shi zhen jing Was a single conclusion based on clinical practice of acupoint after Zhen quan zhen jing(the acupuncture canon of Zhen quan),so it had high documentary and practical val-ues.

  9. 胞质HuR在卵巢上皮性癌的表达及意义%The expression and significance of cytoplasmic HuR in epithelial ovarian carcinoma

    Institute of Scientific and Technical Information of China (English)

    齐玉明; 石彬; 郭皓; 成艳梅

    2011-01-01

    Objective To investigate the correlation between the expression of HuR in epithelial ovarian cancer and clinicopathological parameters in order to explore the factors that affect the prognosis of patients with epithelial ovarian carcinoma. Methods The expressions of cytoplasmic and nuclear HuR in normal ovary tissues,borderline tissue of ovary tumor and epithelial ovarian carcinoma were detected by SP immunohistochemical technique and RT-PCR. The relationship between cytoplasmic HuR expression and prognostic factors was analyzed.Results The expressions of cytoplasmic and nuclear HuR in epithelial ovarian cancer tissue were significantly higher than those in borderline tissue of epithelial ovarian tumor and normal ovarian tissues,however,there was no significant difference between the borderline tissue of epithelial ovarian tumor and normal ovarian tissue ( P >0.05). The statistical analysis showed that the positive expression rate of cytoplasmic HuR in epithelial ovarian carcinoma was closely correlated with histological grade and FIGO stage, but the positive expression rate of nuclear HuR was not correlated with the clinicopathological parameters. Conclusion The over-expression of HuR may play an important role in the growth and progress of epithelial ovarian carcinoma. To detect the expression of cytoplasmic HuR in epithelial ovarian carcinoma may predict the characteristics of oncobiology behaviour. The expression of cytoplasmic HuR may act as an independent prognostic indicator.%目的 检测HuR在卵巢上皮性癌中的表达及与临床病理参数的关系,寻求卵巢癌的预后因素.方法 采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法和逆转录RT-PCR技术,检测胞质和胞核HuR在正常、交界性、恶性卵巢组织中的表达;分析胞质HuR的表达与预后的关系.结果 胞质和胞核HuR在恶性卵巢肿瘤组织的表达明显高于交界性卵巢肿瘤和正常卵巢组织,胞质和胞核HuR在交界性

  10. The Variation of Riverbed Material due to Tropical Storms in Shi-Wen River, Taiwan

    Directory of Open Access Journals (Sweden)

    Chin-Ping Lin

    2014-01-01

    Full Text Available Taiwan, because of its location, is a flood prone region and is characterised by typhoons which brings about two-thirds to three quarters of the annual rainfall amount. Consequently, enormous flows result in rivers and entrain some fractions of the grains that constitute the riverbed. Hence, the purpose of the study is to quantify the impacts of these enormous flows on the distribution of grain size in riverbeds. The characteristics of riverbed material prior to and after the typhoon season are compared in Shi-Wen River located at southern Taiwan. These include grain size variation, bimodality, and roughness coefficient. A decrease (65% and increase (50% in geometric mean size of grains were observed for subsurface and surface bed material, respectively. Geometric standard deviation decreased in all sites after typhoon. Subsurface material was bimodal prior to typhoons and polymodal after. For surface material, modal class is in the gravel class, while after typhoons it shifts towards cobble class. The reduction in geometric mean resulted to a decrease in roughness coefficient by up to 30%. Finally, the relationship of Shields and Froude numbers are studied and a change in the bed form to antidunes and transition form is observed, respectively.

  11. The variation of riverbed material due to tropical storms in Shi-Wen River, Taiwan.

    Science.gov (United States)

    Lin, Chin-Ping; Wang, Yu-Min; Tfwala, Samkele S; Chen, Ching-Nuo

    2014-01-01

    Taiwan, because of its location, is a flood prone region and is characterised by typhoons which brings about two-thirds to three quarters of the annual rainfall amount. Consequently, enormous flows result in rivers and entrain some fractions of the grains that constitute the riverbed. Hence, the purpose of the study is to quantify the impacts of these enormous flows on the distribution of grain size in riverbeds. The characteristics of riverbed material prior to and after the typhoon season are compared in Shi-Wen River located at southern Taiwan. These include grain size variation, bimodality, and roughness coefficient. A decrease (65%) and increase (50%) in geometric mean size of grains were observed for subsurface and surface bed material, respectively. Geometric standard deviation decreased in all sites after typhoon. Subsurface material was bimodal prior to typhoons and polymodal after. For surface material, modal class is in the gravel class, while after typhoons it shifts towards cobble class. The reduction in geometric mean resulted to a decrease in roughness coefficient by up to 30%. Finally, the relationship of Shields and Froude numbers are studied and a change in the bed form to antidunes and transition form is observed, respectively.

  12. Neurologic deterioration with progressive CT changes in a child with Kearns-Shy syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Yoda, Satoru; Kitahara, Fuminori; Akabane, Taro (Shinshu Univ., Matsumoto, Nagano (Japan). Faculty of Medicine); Terauchi, Akiko

    1984-01-01

    A case of the rare juvenile form of Kearns-Shy syndrome with progressive external ophthalmoplegia and lid ptosis, carditis, skeletal muscle weakness, seizures, mental subnormality, short stature, EEG abnormality and deafness is presented. Electromyography revealed a myopathic pattern. Histochemical studies on quadriceps biopsy specimens showed atrophy of type II fibers and ''ragged-red fibers.'' On electron microscopy these muscle cells were seen to contain an increased amount of glycogen particles and abnormal mitochondria were increased in number and size. It is of interest that abrupt deterioration of neurological findings such as seizures, mental subnormality, speech disturbance and deafness was present in our case. Computed tomographic scanning showed progressive changes of cerebral atrophy, low density of cerebral white matter and basal ganglia calcification, which were well associated with the clinical deterioration. A review of the literature also indicated that some patients with this syndrome showed abrupt neurological deterioration in childhood. Involvement of the central nervous system in this syndrome has to be considered as the cause of sudden deterioration and death in childhood.

  13. SiPM readout for the SHiP timing detector

    Science.gov (United States)

    Betancourt, C.; Bezshyiko, I.; Brundler, R.; Serra, N.; Storaci, B.

    2017-02-01

    The SHiP experiment is a new general purpose fixed target experiment proposed at the CERN SPS accelerator. A dedicated beam line in the North Area will be aimed at a fixed target station, followed by a magnetic shield to reduce beam induced background. The experiment will comprise a compact tau neutrino detector and a detector to search for hidden particles. Background rejection is ensured by use of background taggers and a dedicated timing detector. The timing detector will reduce combinatorial di-muon background by requiring incoming particles to be coincident in time within 100 ps. One option for the timing detector consists of columns of horizontal scintillating bars read-out on each end by Silicon Photomultiplier arrays. This study includes characterization of SiPMs from different manufacturers to be used in the timing detector in terms of Current-Voltage behavior, cross-talk probability, dark count rate and single photon timing resolution. Initial results of the time resolution of a EJ-200 scintillating bar read out on both ends by a single SiPM are presented.

  14. Examining the Tendency of Students‧ Shying Away from Departments of Engineering

    Science.gov (United States)

    Taki, Noriko

    Although Japan professes to be a scientific and technological country, more and more students have refused to apply to departments of engineering. From fiscal year 1992 through fiscal year 2008 the number of university applicants dropped to 71.6%. The number of applicants for engineering departments dropped to 61.1%, which is sharper than the decline in the number of total applicants. Among all faculties of national and public universities, engineering departments (first stage of individual exam) have the lowest difficulty level. Almost half of the engineering departments in national and public universities allow more than 50% of their applicants to pass the exam. The gap in difficulty level of entrance examinations between topnotch national universities and rural public universities has been expanding. The difficulty of entrance examination of medical technology departments is much higher than that of engineering ones. However, high-performing students have been enrolling in medical technology departments. For private universities, the gap in the number of engineering applicants between major metropolitan universities and rural ones has become considerable. Almost half of the high school teachers have realized the tendency of students' shying away from engineering departments. The contents of learning of engineering departments are too segmentalzed to understand, which makes it difficult for students to get an image of what they can do in their future after graduation. It is crucial for engineering departments to offer easy-to-understand information on future occupations to junior high students and high school students.

  15. SHI induced modification in structural, optical, dielectric and thermal properties of poly ethylene oxide films

    Energy Technology Data Exchange (ETDEWEB)

    Patel, Gnansagar B.; Bhavsar, Shilpa [Department of Physics, The M.S. University of Baroda, Vadodara 390002 (India); Singh, N.L., E-mail: nl.singh-phy@msubaroda.ac.in [Department of Physics, The M.S. University of Baroda, Vadodara 390002 (India); Singh, F.; Kulriya, P.K. [Inter University Accelerator Centre, Aruna Asaf Ali Marg, New Delhi 110067 (India)

    2016-07-15

    Poly ethylene oxide (PEO) films were synthesized by solution cast method. These self-standing films were exposed with 60 MeV C{sup +5} ion and 100 MeV Ni{sup +7} ion at different fluences. SHI induced effect was investigated by employing various techniques. The crystalline size decreased upon irradiation as observed from XRD analysis. FTIR analysis reveals the decrement in the peak intensity upon irradiation. Tauc’s method was used to determine the optical band gap (E{sub g}), which shows decreasing trends with increase of fluence. The dielectric properties were investigated in the frequency range 10 Hz to 10 MHz for unirradiated and irradiated films. The dielectric constant remains same for the broad-spectrum of frequency and increases at lower frequency. The dielectric loss also moderately influence as a function of frequency due to irradiation. DSC analysis validated the results of XRD. Scanning electron microscopy (SEM) reveals that there is significant change in the surface morphology due to irradiation.

  16. Development and validation of the Shy Bladder and Bowel Scale (SBBS)

    Science.gov (United States)

    Knowles, Simon R.; Skues, Jason

    2016-01-01

    Abstract Currently research exploring paruresis and parcopresis, anxiety relating to urinating and having bowel motions in public respectively is limited. While there are several validated measures of paruresis, no valid measure assessing parcopresis is currently available. The present study investigates the development and validation of the Shy Bladder and Bowel Scale (SBBS) which assesses both paruresis and parcopresis. Two participant groups were utilised to validate this scale, a student psychology cohort (n = 387) and a public cohort (n = 334). An eight-item two-factor model was identified in the psychology cohort and confirmed in the public cohort. The two-factor SBBS was found to be a valid and reliable measure of paruresis and parcopresis. Paruresis and parcopresis-related concerns were associated with social anxiety in both cohorts. Subscales for both paruresis and parcopresis (i.e. difficulty, interference and distress) were positively correlated, suggesting individuals are likely to report similar levels of concerns across both conditions. Further, individuals self-identifying with either paruresis or parcopresis reported significantly higher scores on the respective SBBS subscales than non-identifying paruresis and parcopresis individuals. The SBBS also demonstrated strong test–retest reliability in a small sample of adults (n = 13). Overall, the developed scale provides clinicians and researchers with a valuable tool to evaluate both paruresis and parcopresis. PMID:27216857

  17. AT-RvD1 Promotes Resolution of Inflammation in NOD/ShiLtJ mice

    Science.gov (United States)

    Wang, Ching-Shuen; Maruyama, Christina L.; Easley, Justin T.; Trump, Bryan G.; Baker, Olga J.

    2017-01-01

    Sjögren’s syndrome (SS) is a chronic inflammatory autoimmune disease characterized by diminished secretory function of the exocrine glands. Treatments for hyposalivation are limited to the use of saliva substitutes and medications that provide only temporary relief. In light of the high degree of need and the limitations of current therapies, development of alternative treatments to restore functioning is essential. Resolvins (Rv), which are highly potent lipid mediators, offer a viable alternative for better treating inflammatory diseases such as SS. The goal of this study was to determine whether systemic preventive treatment with Aspirin-triggered RvD1 (AT-RvD1) reduces inflammation and preserves secretory functioning in NOD/ShiLtJ SS-like mice. Our results indicate that systemic treatment with AT-RvD1 diminishes the progression of the disease in salivary epithelium from female mice as follows: (a) improves secretory function, (b) reduces pro-inflammatory molecule gene expression, (c) increases anti-inflammatory molecule gene expression and (d) induces M2 macrophage polarization. Finally, AT-RvD1 decreases lymphocytic infiltration into the salivary glands when used with small doses of the steroid, dexamethasone, and promotes the tissue healing process. PMID:28361884

  18. Investigation of thermally stimulated properties of SHI beam irradiated polycarbonate/polystyrene double layered samples

    Science.gov (United States)

    Rathore, Bhupendra Singh; Gaur, Mulayam Singh; Singh, Kripa Shanker

    2011-12-01

    The double layered samples of polycarbonate/polystyrene (PC/PS) have been prepared by solvent casting method and irradiated with 55 MeV C 5+ beam at different ion fluences range from 1 × 10 11 to 1 × 10 13 ion/cm 2. The effect of swift heavy ion (SHI) beam in interfacial phenomena, phase change, dielectric relaxation, degradation temperature, stability, charge storage and transport mechanism of PC/PS pristine and irradiated double layered samples have been investigated by thermally stimulated discharge current (TSDC), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). TSDC show α, β-relaxation peaks shifted to the lower temperatures side with increase of fluence. The activation energy and relaxation time decrease, while the depolarization current and charge released increase with increase in the ion fluences. DSC curve show the glass transition temperature ( T g) and heat capacity decreases with increase in the ion fluences. The TGA characteristics represent the thermal stability, which is found to be decreased with increase in the ion fluences.

  19. Mechanosensitivity below Ground: Touch-Sensitive Smell-Producing Roots in the Shy Plant Mimosa pudica.

    Science.gov (United States)

    Musah, Rabi A; Lesiak, Ashton D; Maron, Max J; Cody, Robert B; Edwards, David; Fowble, Kristen L; Dane, A John; Long, Michael C

    2016-02-01

    The roots of the shy plant Mimosa pudica emit a cocktail of small organic and inorganic sulfur compounds and reactive intermediates into the environment, including SO2, methanesulfinic acid, pyruvic acid, lactic acid, ethanesulfinic acid, propanesulfenic acid, 2-aminothiophenol, S-propyl propane 1-thiosulfinate, phenothiazine, and thioformaldehyde, an elusive and highly unstable compound that, to our knowledge, has never before been reported to be emitted by a plant. When soil around the roots is dislodged or when seedling roots are touched, an odor is detected. The perceived odor corresponds to the emission of higher amounts of propanesulfenic acid, 2-aminothiophenol, S-propyl propane 1-thiosulfinate, and phenothiazine. The mechanosensitivity response is selective. Whereas touching the roots with soil or human skin resulted in odor detection, agitating the roots with other materials such as glass did not induce a similar response. Light and electron microscopy studies of the roots revealed the presence of microscopic sac-like root protuberances. Elemental analysis of these projections by energy-dispersive x-ray spectroscopy revealed them to contain higher levels of K(+) and Cl(-) compared with the surrounding tissue. Exposing the protuberances to stimuli that caused odor emission resulted in reductions in the levels of K(+) and Cl(-) in the touched area. The mechanistic implications of the variety of sulfur compounds observed vis-à-vis the pathways for their formation are discussed. © 2016 American Society of Plant Biologists. All Rights Reserved.

  20. 周代邿国地望及相关问题再探%A Study of the Locations of Shi State and Other Relevant Issues

    Institute of Scientific and Technical Information of China (English)

    朱继平

    2013-01-01

    This paper reexamines the locations of the Shi State as well as its history in Zhou Dynasty and comes to the conclu-sions as following:1)Originally, Shi State was located in the eastern Pingyin County of Han&Jin Dynasties, which was defen-sive for the southeast frontier of Qi State after Shi State was forced to migrate under the military pressure from Qi State by the middle of the 7th century B.C.2)The new residence for Shi State was located in the southwest of Kangfu County which was very close to Qufu, the capital of Lu State.As a result of this migration, Shi State descended to a client state of Lu State, and Lu State took the opportunity of Shi's inner division in 650 B.C.to destroy it.3) Based on the strategic position of the Shi castle as well as the process of Qi archaeological cultural western expansion, the Shi State destroyed by Lu in 650 B.C.is not around Pingying, but Kangfu, and the former state corresponds to the Xianrentai Cemetery excavated in Changqing District, Jinan.Not only that, given several differences between the tombs of group A and B at Xianrentai Cemetery in distribution, time, tomb structure, character of funerary objects and marriage relationships, the five tombs in group A belong to the Shi State located in Pingyin County during early Spring &Autumn period, and one tomb in group B is likely to relate to the descendents of the Shi State destroyed by Lu State in late Spring&Autumn period.%  针对学界有关认识,综合传世文献与考古发现,对周代邿国地望变迁进行重新梳理,得出以下认识:邿国初位于汉晋平阴以东,因战略位置险要,故在齐势西扩的压力下约于春秋中期早段南迁,在汉晋任城亢父邿亭立国,平阴故地成为齐之邿邑。南迁后邿沦为鲁国附庸,公元前560年因内部分裂而灭于鲁。因此,鲁取之邿并非平阴邿国,而应是南迁后的亢父邿国,山东长清仙人台邿国墓地当对应平阴之邿。

  1. Reversible HuR-microRNA binding controls extracellular export of miR-122 and augments stress response.

    Science.gov (United States)

    Mukherjee, Kamalika; Ghoshal, Bartika; Ghosh, Souvik; Chakrabarty, Yogaditya; Shwetha, Shivaprasad; Das, Saumitra; Bhattacharyya, Suvendra N

    2016-08-01

    microRNAs (miRNAs), the tiny but stable regulatory RNAs in metazoan cells, can undergo selective turnover in presence of specific internal and external cues to control cellular response against the changing environment. We have observed reduction in cellular miR-122 content, due to their accelerated extracellular export in human hepatic cells starved for small metabolites including amino acids. In this context, a new role of human ELAV protein HuR has been identified. HuR, a negative regulator of miRNA function, accelerates extracellular vesicle (EV)-mediated export of miRNAs in human cells. In stressed cells, HuR replaces miRNPs from target messages and is both necessary and sufficient for the extracellular export of corresponding miRNAs. HuR could reversibly bind miRNAs to replace them from Ago2 and subsequently itself gets freed from bound miRNAs upon ubiquitination. The ubiquitinated form of HuR is predominantly associated with multivesicular bodies (MVB) where HuR-unbound miRNAs also reside. These MVB-associated pool of miRNAs get exported out via EVs thereby delimiting cellular miR-122 level during starvation. Therefore, by modulating extracellular export of miR-122, HuR could control stress response in starved human hepatic cells.

  2. SU-E-T-470: Importance of HU-Mass Density Calibration Technique in Proton Pencil Beam Dose Calculation

    Energy Technology Data Exchange (ETDEWEB)

    Penfold, S; Miller, A [University of Adelaide, Adelaide, SA (Australia)

    2015-06-15

    Purpose: Stoichiometric calibration of Hounsfield Units (HUs) for conversion to proton relative stopping powers (RStPs) is vital for accurate dose calculation in proton therapy. However proton dose distributions are not only dependent on RStP, but also on relative scattering power (RScP) of patient tissues. RScP is approximated from material density but a stoichiometric calibration of HU-density tables is commonly neglected. The purpose of this work was to quantify the difference in calculated dose of a commercial TPS when using HU-density tables based on tissue substitute materials and stoichiometric calibrated ICRU tissues. Methods: Two HU-density calibration tables were generated based on scans of the CIRS electron density phantom. The first table was based directly on measured HU and manufacturer quoted density of tissue substitute materials. The second was based on the same CT scan of the CIRS phantom followed by a stoichiometric calibration of ICRU44 tissue materials. The research version of Pinnacle{sup 3} proton therapy was used to compute dose in a patient CT data set utilizing both HU-density tables. Results: The two HU-density tables showed significant differences for bone tissues; the difference increasing with increasing HU. Differences in density calibration table translated to a difference in calculated RScP of −2.5% for ICRU skeletal muscle and 9.2% for ICRU femur. Dose-volume histogram analysis of a parallel opposed proton therapy prostate plan showed that the difference in calculated dose was negligible when using the two different HU-density calibration tables. Conclusion: The impact of HU-density calibration technique on proton therapy dose calculation was assessed. While differences were found in the calculated RScP of bony tissues, the difference in dose distribution for realistic treatment scenarios was found to be insignificant.

  3. Altered regulation of ELAVL1/HuR in HLA-B27-expressing U937 monocytic cells.

    Directory of Open Access Journals (Sweden)

    Anna S Sahlberg

    Full Text Available OBJECTIVE: To investigate the role of HLA-B27 expression in the regulation of RNA binding protein (RBP Embryonic Lethal Abnormal Vision (ELAV L1/Human antigen R (HuR expression in Salmonella-infected or LPS-stimulated human monocytic cells, since HuR is a critical regulator of the post-transcriptional fate of many genes (e.g. TNFα important in inflammatory response. METHODS: U937 monocytic cells were stably transfected with pSV2neo resistant vector (mock, wild type HLA-B27, or mutated HLA-B27 with amino acid substitutions in the B pocket. Cells were differentiated, infected with Salmonella enteritidis or stimulated with lipopolysaccharide. The expression levels of HuR protein and cleavage products (CP1 and CP2 were detected by Western blotting and flow cytometry. Specific inhibitors were used to study the role of PKR and p38 in HuR expression and generation of CPs. TNFα and IL-10 secretion after p38 and PKR inhibition were measured by ELISA. RESULTS: Full length HuR is overexpressed and HuR cleavage is disturbed in U937 monocytic cells expressing HLA-B27 heavy chains (HC. Increased full length HuR expression, disturbed cleavage and reduced dependence on PKR after infection correlate with the expression of glutamic acid 45 in the B pocket that is linked to the misfolding of HLA-B27. CONCLUSION: Results show that the expression of HLA-B27 HCs modulates the intracellular environment of U937 monocyte/macrophages by altering HuR regulation. This phenomenon is at least partly dependent on the misfolding feature of the B27 molecule. Since HuR is an important regulator of multiple genes involved in inflammatory response observations offer an explanation how HLA-B27 may modulate inflammatory response.

  4. Central hypoventilation as the presenting symptom in Hu associated paraneoplastic encephalomyelitis.

    Science.gov (United States)

    Gómez-Choco, Manuel J; Zarranz, Juan J; Saiz, Albert; Forcadas, María I; Graus, Francesc

    2007-10-01

    Central hypoventilation is usually caused by ischaemic or neoplastic lesions of the medulla and upper cervical spinal cord. An autoimmune disorder is not usually considered in the differential diagnosis of this syndrome. We retrospectively identified 14 patients from our database of 202 patients with Hu antibodies who presented with brainstem symptoms. Three were admitted to hospital because of central hypoventilation. All underwent intubation and mechanical ventilation. They could breathe properly while they were awake but suffered deep apnoeas during sleep. Two died, but one is still alive requiring ventilatory assistance during sleep. Autopsy was performed in one of the patients which showed severe inflammatory infiltrates and neuronal loss in the medulla. All patients had normal brain imaging studies and the cause of central hypoventilation was an unsolved problem until Hu antibodies were determined.

  5. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells

    Directory of Open Access Journals (Sweden)

    Xiao-Hong Li

    2013-01-01

    Full Text Available Calcium signals in hepatocytes control cell growth, proliferation, and death. Members of the transient receptor potential (TRP cation channel superfamily are candidate calcium influx channels. NFκB activation strictly depends on calcium influx and often induces antiapoptotic genes favouring cell survival. Previously, we reported that S-[6]-gingerol is an efficacious agonist of the transient receptor potential cation channel subfamily V member 1 (TRPV1 in neurones. In this study, we tested the effect of S-[6]-gingerol on HuH-7 cells using the Fluo-4 calcium assay, RT-qPCR, transient cell transfection, and luciferase measurements. We found that S-[6]-gingerol induced a transient rise in [Ca2+]i in HuH-7 cells. The increase in [Ca2+]i induced by S-[6]-gingerol was abolished by preincubation with EGTA and was also inhibited by the TRPV1 channel antagonist capsazepine. Expression of TRPV1 in HuH-7 cells was confirmed by mRNA analysis as well as a test for increase of [Ca2+]i by TRPV1 agonist capsaicin and its inhibition by capsazepine. We found that S-[6]-gingerol induced rapid NFκB activation through TRPV1 in HuH-7 cells. Furthermore, S-[6]-gingerol-induced NFκB activation was dependent on the calcium gradient and TRPV1. The rapid NFκB activation by S-[6]-gingerol was associated with an increase in mRNA levels of NFκB-target genes: cIAP-2, XIAP, and Bcl-2 that encode antiapoptotic proteins.

  6. Effects of animal class and genotype on beef muscle nanostructure, pHu, colour and tenderness

    Directory of Open Access Journals (Sweden)

    Amanda Y. Chulayo

    2016-07-01

    Full Text Available The objective of the study was to determine the effects of animal class and genotype of cattle on Muscularis longissimus thoracis et lumborum (LTL nanostructure, ultimate pH (pHu, colour and tenderness of beef. We found significant positive relationships between distance travelled (DT and meat temperature (Tm (p"less than"0.01; lairage duration (LDhr and lightness of colour (L* (p"less than"0.01; ambient temperature (Ta and L* (p"less than"0.05 and LDhr and yellowness (b* (p"less than"0.05 of beef from Bonsmara cattle. Positive linear relationships were observed between DT and Tm (p"less than"0.05 and DT and L* (p"less than"0.01 of the non-descript cattle. There were no significant relationships between pre-slaughter stress and other beef quality parameters (pHu, Warner– Bratzler shear force [WBSF], redness [a*] and b* of Bonsmara, Nguni and non-descript cattle. Muscle fibres differed among class and genotype and had an effect on meat quality. The Bonsmara, non-descript and Nguni cows and heifers had visible skeletal muscle fibres which were thin and long, promising improved tenderness of beef. Genotype and class had significant effects on meat quality parameters (Tm, pHu, L*, a*, b* and WBSF. The first important principal components as they appeared from the analysis were pHu, Tm, L*, a*, b* and WBSF. Therefore, animal class did not affect the nanostructure of beef; instead, meat tenderness was enhanced by the longer and visible muscle fibres. Nguni cattle produced meat of superior quality to that of the Bonsmara and the non-descript cattle.

  7. The outcome of PIE *#Hi- and *#Hu- in Germanic

    DEFF Research Database (Denmark)

    Hansen, Bjarne Simmelkjær Sandgaard

    2015-01-01

    It has been established with a great amount of certainty that PIE *#Hu- > Gr. #Vὐ- and that PIE *#Hi- > Gr. #ἰ-. It still remains to be demonstrated what happens in other Western Indo-European branches, including Germanic. In this article , I reject the statement by Ringe (1988: 433) that PIE *#H...... tentatively propose that PG *#au- > PG *#u- when followed by a labial consonant; a development partially paralleled in Greek and in English....

  8. Sustainable groundwater development and management in the Quaternary Hang-Jia-Hu Plain, China

    Institute of Scientific and Technical Information of China (English)

    朱琰

    2002-01-01

    Based on the results of study on regional water supply system, water quality assessments, Quaternary aquifers investigation, and correlation analysis of groundwater depression resulting from land subsidence in the Hang-Jia-Hu Quaternary Plain, this paper presents the groundwater resources policy and sustainable management methods suitable for this area. Suggestions for controlling land subsidence by implementation of wise groundwater policy and management measures are also given.

  9. The outcome of PIE *#Hi- and *#Hu- in Germanic

    DEFF Research Database (Denmark)

    Hansen, Bjarne Simmelkjær Sandgaard

    2015-01-01

    It has been established with a great amount of certainty that PIE *#Hu- > Gr. #Vὐ- and that PIE *#Hi- > Gr. #ἰ-. It still remains to be demonstrated what happens in other Western Indo-European branches, including Germanic. In this article , I reject the statement by Ringe (1988: 433) that PIE *#H...... tentatively propose that PG *#au- > PG *#u- when followed by a labial consonant; a development partially paralleled in Greek and in English....

  10. Screening candidate microRNAs (miRNAs) in different lambskin hair follicles in Hu sheep.

    Science.gov (United States)

    Gao, Wen; Sun, Wei; Yin, Jinfeng; Lv, Xiaoyang; Bao, Jianjun; Yu, Jiarui; Wang, Lihong; Jin, Chengyan; Hu, Liang

    2017-01-01

    Hu sheep lambskin is a unique white lambskin from China that exhibits three types of flower patterns, including small waves, medium waves, and large waves, with small waves considered the best quality. However, our understanding of the molecular mechanism underlying flower pattern formation in Hu sheep lambskin is limited. The aim of the present study was to further explore the relevance between candidate microRNAs (miRNAs) and developmental characteristics of hair follicles and screen miRNAs for later functional validation. Herein, we employed Illumina Hiseq 2500 to identify differentially expressed miRNAs in hair follicles of different flower patterns with small, medium, and large waves to construct a comprehensive sequence database on the mechanism of hair follicle development. Paraffin sections of lambskin tissue were prepared to assess the structure of different hair follicles. Expression levels of candidate miRNAs in different flower patterns were analyzed by relative quantitation using real-time PCR, combined with histological observation and micro-observation technologies, and the correlation between expression levels of candidate miRNAs and histological properties of hair follicles was analyzed by using SPSS 17.0. A total of 522 differentially expressed miRNAs were identified, and RNA-seq analysis detected 7,266 target genes in different groups of flower patterns. Gene ontological analysis indicated these target genes were mainly involved in cell proliferation, differentiation, growth, apoptosis, and ion transport, and 14 miRNAs, including miR-143, miR-10a, and let-7 were screened as candidate miRNAs in Hu sheep hair follicle growth and development. In the same field of vision, variance analysis showed that the number of secondary follicles in small waves was significantly larger than that in large and medium waves (Phair follicles, highly significant differences in miRNA-143 expression levels between large and small waves were observed (Phair follicle

  11. Pragmatists, positive communicators, and shy enthusiasts: three viewpoints on Web conferencing in health sciences education.

    Science.gov (United States)

    Valaitis, Ruta; Akhtar-Danesh, Noori; Eva, Kevin; Levinson, Anthony; Wainman, Bruce

    2007-12-31

    Web conferencing is a synchronous technology that allows coordinated online audio and visual interactions with learners logged in to a central server. Recently, its use has grown rapidly in academia, while research on its use has not kept up. Conferencing systems typically facilitate communication and support for multiple presenters in different locations. A paucity of research has evaluated synchronous Web conferencing in health sciences education. McMaster University Faculty of Health Sciences trialed Wimba's Live Classroom Web conferencing technology to support education and curriculum activities with students and faculty. The purpose of this study was to explore faculty, staff, and student perceptions of Web conferencing as a support for teaching and learning in health sciences. The Live Classroom technology provided features including real-time VoIP audio, an interactive whiteboard, text chat, PowerPoint slide sharing, application sharing, and archiving of live conferences to support student education and curriculum activities. Q-methodology was used to identify unique and common viewpoints of participants who had exposure to Web conferencing to support educational applications during the trial evaluation period. This methodology is particularly useful for research on human perceptions and interpersonal relationships to identify groups of participants with different perceptions. It mixes qualitative and quantitative methods. In a Q-methodology study, the goal is to uncover different patterns of thought rather than their numerical distribution among the larger population. A total of 36 people participated in the study, including medical residents (14), nursing graduate students (11), health sciences faculty (9), and health sciences staff (2). Three unique viewpoints were identified: pragmatists (factor 1), positive communicators (factor 2A), and shy enthusiasts (factor 2B). These factors explained 28% (factor 1) and 11% (factor 2) of the total variance

  12. Downregulation of β-Adrenoceptors in Isoproterenol-Induced Cardiac Remodeling through HuR.

    Directory of Open Access Journals (Sweden)

    Qian Yin

    Full Text Available β-adrenergic receptors (β-ARs play an important role in cardiac remodeling, which is the key pathological process in various heart diseases and leads to heart failure. However, the regulation of β-AR expression in remodeling hearts is still unclear. This study aims to clarify the possible mechanisms underlying the regulation of β1- and β2-AR expression in cardiac remodeling. The rat model of cardiac remodeling was established by subcutaneous injection of isoproterenol(ISO at the dose of 0.25 mg·kg(-1·d(-1 for 7 days. We found that the expression of β1- and β2-ARs decreased in the remodeling heart. The mechanisms may include the inhibition of DNA transcription and the increase of mRNA degradation. cAMP-response element binding protein(CREB is a well-known transcription factor of β-AR. However, the expression and activation of CREB was not changed in the remodeling heart. Further, human Antigen-R (HuR, a RNA binding protein, which binds to the 3'-untranslated region of the β-AR mRNA and promotes RNA degradation, was increased in the remodeling model. And in vitro, HuR deficiency reversed the reduction of β-AR mRNA induced by ISO. Therefore, the present findings indicate that HuR, but not CREB, is responsible for the reduction of β-AR expression in ISO induced cardiac remodeling.

  13. Intraperirhinal cortex administration of the synthetic cannabinoid, HU210, disrupts object recognition memory in rats.

    Science.gov (United States)

    Sticht, Martin A; Jacklin, Derek L; Mechoulam, Raphael; Parker, Linda A; Winters, Boyer D

    2015-03-25

    Cannabinoids disrupt learning and memory in human and nonhuman participants. Object recognition memory, which is particularly susceptible to the impairing effects of cannabinoids, relies critically on the perirhinal cortex (PRh); however, to date, the effects of cannabinoids within PRh have not been assessed. In the present study, we evaluated the effects of localized administration of the synthetic cannabinoid, HU210 (0.01, 1.0 μg/hemisphere), into PRh on spontaneous object recognition in Long-Evans rats. Animals received intra-PRh infusions of HU210 before the sample phase, and object recognition memory was assessed at various delays in a subsequent retention test. We found that presample intra-PRh HU210 dose dependently (1.0 μg but not 0.01 μg) interfered with spontaneous object recognition performance, exerting an apparently more pronounced effect when memory demands were increased. These novel findings show that cannabinoid agonists in PRh disrupt object recognition memory.

  14. 论胡锦涛的人才观%On HuJintao' s View of Talent

    Institute of Scientific and Technical Information of China (English)

    于伟峰; 黄静

    2012-01-01

    胡锦涛的人才观主要体现在:树立人才资源为第一资源、人人皆可成才和以人为本的观念。胡锦涛的人才观的核心内容与前三代领导集体人才观的核心内容既一眯相承又有其独特的创新点,他首次提出了党管人才的原则和将人才观与以人为本的发展观相结合的原则。%Hu Jintao' s view of talents is embodied in 1 ) his view that human resources are paramount to all other resources; 2) his belief that everyone can develop into a man of caliber; and 3) his people-oriented approach to development. The kernel of Hu Jintao' s view of talent is imbued with the same spirit reflected in the general out- look on talent of previous three generations of central collective leadership of the CPC, while boasting unique inno- vations. Hu first puts forward the principle of managing talents by the party and the principle of combining the sci- entific view of talent with the people-oriented approach to development.

  15. Anti-solar differential rotation on the active sub-giant HU Virginis

    CERN Document Server

    Harutyunyan, G; Künstler, A; Carroll, T A; Weber, M

    2016-01-01

    Measuring surface differential rotation (DR) on different types of stars is important when characterizing the underlying stellar dynamo. It has been suggested that anti-solar DR laws can occur when strong meridional flows exist. We aim to investigate the differential surface rotation on the primary star of the RS CVn binary HU Vir by tracking its starspot distribution as a function of time. We also aim to recompute and update the values for several system parameters of the triple system HU Vir (close and wide orbits). Time-series high-resolution spectroscopy for four continuous months was obtained with the 1.2-m robotic STELLA telescope. Nine consecutive Doppler images were reconstructed from these data, using our line-profile inversion code iMap. An image cross-correlation method was applied to derive the surface differential-rotation law for HU Vir. New orbital elements for the close and the wide orbits were computed using our new STELLA radial velocities (RVs) combined with the RV data available in the lit...

  16. CTXφ Replication Depends on the Histone-Like HU Protein and the UvrD Helicase.

    Directory of Open Access Journals (Sweden)

    Eriel Martínez

    2015-05-01

    Full Text Available The Vibrio cholerae bacterium is the agent of cholera. The capacity to produce the cholera toxin, which is responsible for the deadly diarrhea associated with cholera epidemics, is encoded in the genome of a filamentous phage, CTXφ. Rolling-circle replication (RCR is central to the life cycle of CTXφ because amplification of the phage genome permits its efficient integration into the genome and its packaging into new viral particles. A single phage-encoded HUH endonuclease initiates RCR of the proto-typical filamentous phages of enterobacteriaceae by introducing a nick at a specific position of the double stranded DNA form of the phage genome. The rest of the process is driven by host factors that are either essential or crucial for the replication of the host genome, such as the Rep SF1 helicase. In contrast, we show here that the histone-like HU protein of V. cholerae is necessary for the introduction of a nick by the HUH endonuclease of CTXφ. We further show that CTXφ RCR depends on a SF1 helicase normally implicated in DNA repair, UvrD, rather than Rep. In addition to CTXφ, we show that VGJφ, a representative member of a second family of vibrio integrative filamentous phages, requires UvrD and HU for RCR while TLCφ, a satellite phage, depends on Rep and is independent from HU.

  17. Cannabinoids & Stress: impact of HU-210 on behavioral tests of anxiety in acutely stressed mice.

    Science.gov (United States)

    Kinden, Renee; Zhang, Xia

    2015-05-01

    Anxiety disorders are one of the most prevalent classes of mental disorders affecting the general population, but current treatment strategies are restricted by their limited efficacy and side effect profiles. Although the cannabinoid system is speculated to be a key player in the modulation of stress responses and emotionality, the vast majority of current research initiatives had not incorporated stress exposure into their experimental designs. This study was the first to investigate the impact of exogenous cannabinoid administration in an acutely stressed mouse model, where CD1 mice were pre-treated with HU-210, a potent CB1R agonist, prior to acute stress exposure and subsequent behavioral testing. Exogenous cannabinoid administration induced distinct behavioral phenotypes in stressed and unstressed mice. While low doses of HU-210 were anxiolytic in unstressed subjects, this effect was abolished when mice were exposed to an acute stressor. The administration of higher HU-210 doses in combination with acute stress exposure led to severe locomotor deficits that were not previously observed at the same dose in unstressed subjects. These findings suggest that exogenous cannabinoids and acute stress act synergistically in an anxiogenic manner. This study underlies the importance of including stress exposure into future anxiety-cannabinoid research due to the differential impact of cannabinoid administration on stressed and unstressed subjects.

  18. Clinical study on intravitreal injection of cannabinoid HU-211 for optic nerve damage in glaucoma rats

    Directory of Open Access Journals (Sweden)

    Hui-Feng Liu

    2014-09-01

    Full Text Available AIM: To evaluate the protective effects of intravitreal injection of cannabinoid HU-211 for optic nerve in glaucoma rats, providing the experimental evidence in the treatment of glaucoma optic nerve injury.METHODS: Glaucoma rats(18 eyeswere modeled by electric coagulation sclera surface vein and randomized into 3 groups, group A received intravitreal injection of 1mg/0.1mL cannabinoid HU-211 every other day respectively; group B was given intravitreal injection of 0.1mL water every other day, group C was high intraocular pressure(IOPgroup, 6 eyes were randomly selected for blank control group(group D. IOP was observed every day. The rats were sacrificed after treatment 4wk, froze retina section, HE stain. The density fluctuation of retinal ganglion cell(RGCneurons assessment the optic nerve of rat model with chronic high IOP glaucoma were measured.RESULTS: The apoptosis and damage degree of RGC in group B was obviously higher than that in group A, with statistically significant difference(PP >0.05.CONCLUSION: Intravitreal injection of cannabinoid HU-211 shows obvious protective effect on optic nerve in glaucom rat models.

  19. The cytoskeletal inhibitors latrunculin A and blebbistatin exert antitumorigenic properties in human hepatocellular carcinoma cells by interfering with intracellular HuR trafficking

    Energy Technology Data Exchange (ETDEWEB)

    Doller, Anke; Badawi, Amel [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Schmid, Tobias; Brauß, Thilo [Institut für Biochemie I (Pathobiochemie), Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Pleli, Thomas [Medizinische Klinik 1, Schwerpunkt Gastroenterologie und Hepatologie, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Meyer zu Heringdorf, Dagmar [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Piiper, Albrecht [Medizinische Klinik 1, Schwerpunkt Gastroenterologie und Hepatologie, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Pfeilschifter, Josef [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Eberhardt, Wolfgang, E-mail: w.eberhardt@em.uni-frankfurt.de [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany)

    2015-01-01

    The impact of the RNA-binding protein HuR for the post-transcriptional deregulation of tumor-relevant genes is well established. Despite of elevations in HuR expression levels, an increase in cytoplasmic HuR abundance in many cases correlates with a high grade of malignancy. Here, we demonstrated that administration of the actin-depolymerizing macrolide latrunculin A, or blebbistatin, an inhibitor of myosin II ATPase activity, caused a dose- and time-dependent reduction in the high cytoplasmic HuR content of HepG2 and Huh7 hepatocellular carcinoma (HCC) cells. Subcellular fractionation revealed that in addition, both inhibitors strongly attenuated cytoskeletal and membrane-bound HuR abundance and conversely increased the HuR amount in nuclear cell fractions. Concomitant with changes in intracellular HuR localization, both cytoskeletal inhibitors markedly decreased the half-lives of cyclooxygenase-2 (COX-2), cyclin A and cyclin D{sub 1} encoding mRNAs resulting in a significant reduction in their expression levels in HepG2 cells. Importantly, a similar reduction in the expression of these HuR targets was achieved by a RNA interference (RNAi)-mediated knockdown of either HuR or nonmuscle myoin IIA. Using polysomal fractionation, we further demonstrate that the decrease in cytoplasmic HuR by latrunculin A or blebbistatin is accompanied by a marked change in the allocation of HuR and its mRNA cargo from polysomes to ribonucleoprotein (RNP) particles. Functionally, the basal migration and prostaglandin E{sub 2} synthesis are similarly impaired in inhibitor-treated and stable HuR-knockdown HepG2 cells. Our data demonstrate that interfering with the actomyosin-dependent HuR trafficking may comprise a valid therapeutic option for antagonizing pathologic posttranscriptional gene expression by HuR and furthermore emphasize the potential benefit of HuR inhibitory strategies for treatment of HCC. - Highlights: • We tested the effects of latrunculin A and blebbistatin on

  20. High-level Expression and Purification of Human Zona Pellucida huZP3a22-176 and huZP3b177-348 Peptides in Escherichia coli

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Objective To try making huZP3a22-176 and huZP3b177-348 polypeptides (representing an intact huZP322-348 protein without its N-terminal signal peptide and C-terminal transmembrane domain ) express in E. coli at a higher levelMethods The cDNAs encoding huZP3a and huZP3b were obtained with PCR method.The pB V221 plasmid was used to construct thermo-inducible recombinant expression vector. Purification of two target expression products employed an improved method of preparative gel polyacrylamide gel electrophoresis.Results Two polypeptides of recombinant huZP3a (rhuZP3a) and recombinant huZP3b(rhuZP3b) were all expressed respectively in an E. coli BL21(DE3)pLysS strain at a higher level, which were recognized by two specific polyclonal antisera in Western blotting test which recognize a linear B cell epitope present in rhuZP3a or rhuZP3b respectively. Using the shake-flask method, approximately 5 mg of rhuZP3a and rhuZP3b with more than 95% relative homogeneity were harvested from 1 L culture respectively.Conclusion The availability of two rhuZP3 polypeptides will help in detecting the immunogenicities of rhuZP3a and rhuZP3b through animal experiments and confirming the function domain of non-glycosylated huZP3 to induce acrosome reaction in vitro.

  1. Isolation, purification, crystallization, and preliminary X-ray diffraction study of the crystals of HU protein from M. gallisepticum

    Science.gov (United States)

    Nikolaeva, A. Yu.; Timofeev, V. I.; Boiko, K. M.; Korzhenevskii, D. A.; Rakitina, T. V.; Dorovatovskii, P. V.; Lipkin, A. V.

    2015-11-01

    HU proteins are involved in bacterial DNA and RNA repair. Since these proteins are absent in cells of higher organisms, inhibitors of HU proteins can be used as effective and safe antibiotics. The crystallization conditions for the M. gallisepticum HU protein were found and optimized by the vapor-diffusion method. The X-ray diffraction data set was collected to 2.91 Å resolution from the crystals grown by the vapor-diffusion method on a synchrotron source. The crystals of the HU protein belong to sp. gr. P41212 and have the following unit-cell parameters: a = b = 97.94 Å, c = 77.92 Å, α = β = γ = 90°.

  2. Homologues of the Arabidopsis thaliana SHI/STY/LRP1 genes control auxin biosynthesis and affect growth and development in the moss Physcomitrella patens.

    Science.gov (United States)

    Eklund, D Magnus; Thelander, Mattias; Landberg, Katarina; Ståldal, Veronika; Nilsson, Anders; Johansson, Monika; Valsecchi, Isabel; Pederson, Eric R A; Kowalczyk, Mariusz; Ljung, Karin; Ronne, Hans; Sundberg, Eva

    2010-04-01

    The plant hormone auxin plays fundamental roles in vascular plants. Although exogenous auxin also stimulates developmental transitions and growth in non-vascular plants, the effects of manipulating endogenous auxin levels have thus far not been reported. Here, we have altered the levels and sites of auxin production and accumulation in the moss Physcomitrella patens by changing the expression level of homologues of the Arabidopsis SHI/STY family proteins, which are positive regulators of auxin biosynthesis genes. Constitutive expression of PpSHI1 resulted in elevated auxin levels, increased and ectopic expression of the auxin response reporter GmGH3pro:GUS, and in an increased caulonema/chloronema ratio, an effect also induced by exogenous auxin application. In addition, we observed premature ageing and necrosis in cells ectopically expressing PpSHI1. Knockout of either of the two PpSHI genes resulted in reduced auxin levels and auxin biosynthesis rates in leafy shoots, reduced internode elongation, delayed ageing, a decreased caulonema/chloronema ratio and an increased number of axillary hairs, which constitute potential auxin biosynthesis sites. Some of the identified auxin functions appear to be analogous in vascular and non-vascular plants. Furthermore, the spatiotemporal expression of the PpSHI genes and GmGH3pro:GUS strongly overlap, suggesting that local auxin biosynthesis is important for the regulation of auxin peak formation in non-vascular plants.

  3. HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells

    Directory of Open Access Journals (Sweden)

    Ennifar Eric

    2008-06-01

    Full Text Available Abstract Reverse transcription of the genetic material of human immunodeficiency virus type 1 (HIV-1 is a critical step in the replication cycle of this virus. This process, catalyzed by reverse transcriptase (RT, is well characterized at the biochemical level. However, in infected cells, reverse transcription occurs in a multiprotein complex – the reverse transcription complex (RTC – consisting of viral genomic RNA associated with viral proteins (including RT and, presumably, as yet uncharacterized cellular proteins. Very little is known about the cellular proteins interacting with the RTC, and with reverse transcriptase in particular. We report here that HIV-1 reverse transcription is affected by the levels of a nucleocytoplasmic shuttling protein – the RNA-binding protein HuR. A direct protein-protein interaction between RT and HuR was observed in a yeast two-hybrid screen and confirmed in vitro by homogenous time-resolved fluorescence (HTRF. We mapped the domain interacting with HuR to the RNAse H domain of RT, and the binding domain for RT to the C-terminus of HuR, partially overlapping the third RRM RNA-binding domain of HuR. HuR silencing with specific siRNAs greatly impaired early and late steps of reverse transcription, significantly inhibiting HIV-1 infection. Moreover, by mutagenesis and immunoprecipitation studies, we could not detect the binding of HuR to the viral RNA. These results suggest that HuR may be involved in and may modulate the reverse transcription reaction of HIV-1, by an as yet unknown mechanism involving a protein-protein interaction with HIV-1 RT.

  4. MeSHy: Mining unanticipated PubMed information using frequencies of occurrences and concurrences of MeSH terms.

    Science.gov (United States)

    Theodosiou, T; Vizirianakis, I S; Angelis, L; Tsaftaris, A; Darzentas, N

    2011-12-01

    PubMed is the most widely used database of biomedical literature. To the detriment of the user though, the ranking of the documents retrieved for a query is not content-based, and important semantic information in the form of assigned Medical Subject Headings (MeSH) terms is not readily presented or productively utilized. The motivation behind this work was the discovery of unanticipated information through the appropriate ranking of MeSH term pairs and, indirectly, documents. Such information can be useful in guiding novel research and following promising trends. A web-based tool, called MeSHy, was developed implementing a mainly statistical algorithm. The algorithm takes into account the frequencies of occurrences, concurrences, and the semantic similarities of MeSH terms in retrieved PubMed documents to create MeSH term pairs. These are then scored and ranked, focusing on their unexpectedly frequent or infrequent occurrences. MeSHy presents results through an online interactive interface facilitating further manipulation through filtering and sorting. The results themselves include the MeSH term pairs, along with MeSH categories, the score, and document IDs, all of which are hyperlinked for convenience. To highlight the applicability of the tool, we report the findings of an expert in the pharmacology field on querying the molecularly-targeted drug imatinib and nutrition-related flavonoids. To the best of our knowledge, MeSHy is the first publicly available tool able to directly provide such a different perspective on the complex nature of published work. Implemented in Perl and served by Apache2 at http://bat.ina.certh.gr/tools/meshy/ with all major browsers supported. Copyright © 2011 Elsevier Inc. All rights reserved.

  5. Domain-specific phosphomimetic mutation allows dissection of different protein kinase C (PKC) isotype-triggered activities of the RNA binding protein HuR.

    Science.gov (United States)

    Schulz, Sebastian; Doller, Anke; Pendini, Nicole R; Wilce, Jacqueline A; Pfeilschifter, Josef; Eberhardt, Wolfgang

    2013-12-01

    The ubiquitous mRNA binding protein human antigen R (HuR) participates in the post-transcriptional regulation of many AU-rich element (ARE)-bearing mRNAs. Previously, by using in vitro kinase assay, we have identified serines (Ser) 158, 221 and 318 as targets of protein kinase C (PKC)-triggered phosphorylation. In this study, we tested whether GFP- or GST-tagged HuR constructs bearing a phosphomimetic Ser (S)-to-Asp (D) substitution at the different PKC target sites, would affect different HuR functions including HuR nucleo-cytoplasmic redistribution and binding to different types of ARE-containing mRNAs. The phosphomimetic GFP-tagged HuR protein bearing a phosphomimetic substitution in the hinge region of HuR (HuR-S221D) showed an increased cytoplasmic abundance when compared to wild-type HuR. Conversely, data from in vitro kinase assay and electrophoretic mobility shift assay (EMSA), implicates that phosphorylation at Ser 221 is not relevant for mRNA binding of HuR. Quantification of in vitro binding affinities of GST-tagged wild-type HuR and corresponding HuR proteins bearing a phosphomimetic substitution in either RRM2 (HuR-S158D) or in RRM3 (HuR-S318D) by microscale thermophoresis (MST) indicates a specific binding of wild-type HuR to type I, II or type III-ARE-oligonucleotides in the high nanomolar range. Interestingly, phosphomimetic mutation at position 158 or 318 had a negative influence on HuR binding to type I- and type II-ARE-mRNAs whereas it significantly enhanced HuR affinity to a type III-ARE substrate. Our data suggest that differential phosphorylation of HuR by PKCs at different HuR domains coordinates subcellular HuR distribution and leads to a preferential binding to U-rich bearing target mRNA.

  6. A Review of the Pharmacological Effects of the Dried Root of Polygonum cuspidatum (Hu Zhang and Its Constituents

    Directory of Open Access Journals (Sweden)

    Huan Zhang

    2013-01-01

    Full Text Available Traditional Chinese medicine (TCM has been widely used in China for thousands of years to treat and prevent diseases. TCM has been proven safe and effective, and it is being considered as one of the important types of complementary and alternative medicine and receives increasing attention worldwide. The dried root of Polygonum cuspidatum Sieb. et Zucc. (also known as “Hu Zhang” in Chinese is one of the medicinal herbs listed in the Pharmacopoeia of the People's Republic of China. Hu Zhang is widely distributed in the world. It can be found in Asia and North America and is used as folk medicine in countries such as Japan and Korea. In China, Hu Zhang is usually used in combination with other TCM herbs. The therapeutic uses of those Hu Zhang-containing TCM prescriptions or formulations are for treating cough, hepatitis, jaundice, amenorrhea, leucorrhea, arthralgia, burns and snake bites. Recent pharmacological and clinical studies have indicated that Hu Zhang has antiviral, antimicrobial, anti-inflammatory, neuroprotective, and cardioprotective functions. This review gives a summary of the reported therapeutic effects of the active compounds and the different extracts of Hu Zhang.

  7. Violence against women in the context of war: experiences of Shi'i women and Palestinian refugee women in Lebanon.

    Science.gov (United States)

    Holt, Maria

    2013-03-01

    In times of war, women are likely to experience, in addition to the "normal" violence of peacetime, random cruelties perpetrated by the enemy against all members of the community. During research conducted with Palestinian refugees and Shi'i Muslims in Lebanon, women described various forms of violence and, in this article, I examine violence suffered by women in the context of conflict from three perspectives: victimization, trauma, and resistance. I argue that traumatic events have the effect of obliterating identity, but they can also strengthen the resolve to resist.

  8. When people in close relationships are not prepared to listen to emotional disclosures. The role of social constraints in shy people’s functioning

    Directory of Open Access Journals (Sweden)

    Irena Dzwonkowska

    2007-12-01

    Full Text Available The following article comprises a presentation of research carried out on a group of 268 adults. The survey aimed at finding answers to questions posed about the meditative role played by social constraints in the relationship between shyness and certain aspects of emotional and social functioning. The results indicate that social constraints are a destructive factor in the everyday functioning of those facing everyday problems. Many shy people experience social constraints - people in close relationships: family, relatives, and friends react inadequately and negatively, demonstrating a lack of empathy, thus discouraging people who are shy from expressing their personal thoughts and emotions. Regression analyses, conducted in order to detect the meditative effects of social constraints, show that their destructive influence is particularly severe in the case of shy people leading to their low self-esteem, a high level of depressive symptoms and a low perception of social support.

  9. "He is just a little shy like me": screening for autism in a young child.

    Science.gov (United States)

    Weissman, Laura; Sices, Laura; Augustyn, Marilyn

    2010-10-01

    accountant and father as a physicist in a commercial laboratory. Both have graduate degrees and are trilingual. The parents are conflicted in their interpretation of Jacob's behavior. His father is concerned that he is autistic and his mother feels that "he is just a little shy like me." His physical examination is normal. He is somewhat inhibited and anxious but will sit still on the examination table and alone during the examination. You update his immunizations and then struggle with where to head next.

  10. Chromatographic isolation of nanoparticles from Ma-Xing-Shi-Gan-Tang decoction and their characterization.

    Science.gov (United States)

    Zhou, Jianwu; Gao, Guanzhen; Chu, Qiuping; Wang, Huiqin; Rao, Pingfan; Ke, Lijing

    2014-02-12

    The herbal decoction is a complex dispersion system containing solutes, colloid, aggregates, emulsions and precipitates. In which phase bioactive phytochemicals are dispersed determines their delivery, action and metabolism. This study took ephedrine, a well-studied and widely used phytochemical, as an example to elucidate its exact distribution in the phases of Ma-Xing-Shi-Gan-Tang decoction (MXSGT), which is an Ephedra sinica Stapf. containing traditional Chinese medicinal formula, and the biological meaning of this distribution correspondingly. It may provide an important update to the safety and efficacy assessment of the herbal decoction and its active phytochemicals. In this study, the decoction was fractionated with size-exclusion chromatography coupled with multi-angle laser light scattering detector. The morphology of fractionated nanoparticles was observed with AFM and SEM. The bioactivities of the decoction, the ephedrine alkaloids loaded NPs (prepared by chromatography isolation) and the synthetic ephedrine were assessed by cell proliferation tests using five cell lines, namely Caco-2, L-02, Hep-G2, NR-8383, and Hela-229. Nanoparticles with radii of gyration ranged from 50 to 150 nm were isolated, in spherical shape. Further analysis of nanoparticles on the subsequent reversed phase chromatography revealed that the majority of ephedrine (99.7%) and pseudoephedrine (95.5%) were associated with these nanoparticles, rather than dispersed freely in the real solution. The addition of both the herbal decoction and the separated ephedrine-loaded nanoparticles reserved higher cell viability/proliferation than that of the sole synthetic ephedrine among the Caco-2, L-02, Hep-G2, and NR-8383 cells. In contrast, the nanoparticles reduced the proliferating power of ephedrine on Hela-229 cells. In general, the ephedrine-loaded NPs conducted the intermediate influences on the cell viability, in either way. The colloidal nanoparticles were separated from the decoction

  11. 论胡适古典小说考证的西学背景%On Western Academic Background of Hu Shi’s Textural Research on Classical Novels

    Institute of Scientific and Technical Information of China (English)

    王光和

    2012-01-01

    The modern consciousness of Hu Shi's literary criticism mainly came from western cultural thoughts.These thoughts contained ideological trend of scientism,philosophy of positivism and methods of western textual criticism,as well as criticism mode of positivism in literary criticism.His achievements on literary criticism were mainly in textural researches on classical novels,with the climax of establishing the neo-redology.His style of "understanding human beings and reflecting on society" in textural research on novels did not only improve the traditional methods,but also absorb the advantages of western criticism of positivism,which had some effect on the later literary criticism.%胡适文学批评所呈现的现代意识更多的是因为吸收西方文化思想而形成的。这些西方文化思想既包括科学主义思潮、实证主义哲学、西方校勘学方法等,也包括文学批评中的实证主义批评模式。胡适的文学批评成绩主要表现在传统小说的考证当中,其最高成就体现在"新红学"的开创。其小说考证的"知人论世"的风格既是对传统的承继和提升,也是对西方实证主义批评的有效吸纳,它对后来的文学批评产生了一定的影响。

  12. CONSTRUCTION OF HU-PBL/SCID CHIMERAS AND DEVELOPMENT OF EBV-RELATED LYMPHOMAS

    Institute of Scientific and Technical Information of China (English)

    Run-liang Gan; Ke Lan; Zhi-hua Yin; Li-jiang Wang; Ying Song; Kai-tai Yao

    2005-01-01

    Objective To construct hu-PBL/SCID chimeras and to investigate the development of lymphoma and oncogenicity of the Epstein-Barr virus (EBV).Mtehods Human peripheral blood lymphocytes (PBLs) were isolated from healthy adult donors and transplanted intraperitoneally into severe combined immunodeficient (SCID) mice. Mice with hu-PBL engraftment from healthy EBV seronegative donors were injected intraperitoneally with EBV-containing supematant from suspension culture of B95-8 cell line (active infection), whereas mice receiving lymphocytes from healthy EBV seropositive donors were not re-infected with B95-8 derived EBV (latent infection). Pathological examination and molecular analysis were performed on experimental animals and induced neoplasms.Results In the early stage of this experiment, 12 mice died of acute graft-versus-host disease, mortality was 34.3%(12/35 mice) with an average life span of 17.5 days. In 19 survival hu-PBL/SCID chimeric recipients from 12 healthy donors,tumor incidence was 84.2% (16/19 mice). The average survival time of tumor-bearing mice was 65.5 days. EBV-related neoplasms in SCID mice were nodular tumors with aggressive and fatal features. Histological morphology of tumors exhibited diffuse large cell lymphomas. Immunohistochemistry revealed that LCA (CD45) and L26 (CD20) were positive, but both PS1 (CD3) and UCHL-1 (CD45RO) were negative, and EBV products ZEBRA, LMP1, and EBNA2 were expressed in a small number of tumor cells. EB virus particles were seen in the nuclei of some tumor cells by electron microscopy, and EBV DNA could be amplified in the tumor tissues by PCR. In situ hybridization indicated that the nuclei of tumor cells contained human-specific Alu sequence.Conclusions EBV-induced tumors were human B-cell malignant lymphomas. We obtained direct causative evidence dealing with EBV-associated tumor deriving from normal human cells.

  13. 'Hu Hong' (bad thing): parental perceptions of teenagers' sexuality in urban Vietnam.

    Science.gov (United States)

    Do, Lan Anh Thi; Boonmongkon, Pimpawun; Paek, Seung Chun; Guadamuz, Thomas E

    2017-02-28

    Teenagers under 18 years old in Vietnam are considered as minors who usually lack the autonomy to make decisions. They are also sometimes viewed as contributors to social evils including crime, violence and substance use. Moreover, most Vietnamese teenagers have unsafe sex before marriage. The objective of this study is to explore the parental perceptions relating to their teenagers' sexuality, particularly the social and cultural forces, that may hinder access to sexuality information. Guided by a Community Advisory Board (CAB), this qualitative study uses four focus group discussions (FGDs) consisting of 12 mothers and 12 fathers, as well as twelve individual in-depth interviews (IDIs) with a diverse sample of parents of teens in Ho Chi Minh City (HCMC), Vietnam. Content and discourse analysis were conducted, based on Foucauldian concepts. Four themes emerged: 1) Meanings of sexuality and sexuality education, 2) Early sexual intercourse destroys teenagers' future, 3) Teenagers are not hu hong (spoil/bad thing), are innocent and virgin, and 4) Policing and controlling of sexual intercourse among teens. Parents did not view their teenage children as sexual beings; those who are sexual are considered hu hong. Parents believed that teens need to be policed and controlled to prevent them from becoming hu hong, particularly girls. Controlling of sexuality information by parents was therefore common in HCMC, but differed by gender and educational levels of parents. For example, fathers more than mothers were not comfortable teaching their teenage children about sex and sexuality. Parents with higher education police their teenage children's usage of the Internet and social media, while parents with lower education control who can be friends with their teenage children. Vietnamese parents in general have negative views of sex and sexuality education for their teenage children. Recognizing that many Vietnamese teenagers have unsafe sex before marriage, parents need to

  14. Effects of weaning age on growth, nutrient digestibility and metabolism, and serum parameters in Hu lambs

    Directory of Open Access Journals (Sweden)

    Jianmin Chai

    2015-12-01

    Full Text Available This study was conducted to investigate the effect of weaning age on growth performance, nutrient digestion and metabolism, and serological indicators, and to obtain an optimal weaning age in Hu lambs. Forty-eight newborn Hu lambs (birth weight, 2.53 ± 0.14 kg were randomly divided into 4 groups. The lambs in control group (ER suckled their dams. The lambs in other three experimental groups were weaned on milk replacer at 10, 20, and 30 days of age (EW10, EW20, and EW30 groups, respectively. The results were as follows: 1 lambs in EW10 and EW30 groups had a lower (P  0.05 among groups; however, the apparent digestibility and deposition of calcium in early weaned lambs were lower (P < 0.05 than those in ewe-reared lambs. 4 The albumin content in EW30 group was lower (P < 0.05 than that in ER group; the globulin content in EW30 group was higher (P < 0.05 than that in other groups; the content of serum insulin-like growth factor-Ⅰ in weaned lambs tended to increase compared with lambs in ER group. Finally, the growth rate of lambs decreased within 10 days post-weaning, but early weaning boosted creep feed intake, leading to better growth and health later in life. The Hu lambs can be weaned on milk replacer and creep feed at 10 days of age.

  15. SHyPIE: a new source for on-line production of multicharged radioactive condensable ion beams

    Energy Technology Data Exchange (ETDEWEB)

    Lecesne, N.; Foury, P.; Gaubert, G.; Huguet, Y.; Jardin, P.; Leroy, R.; Lewitowicz, M.; Marry, C.; Maunoury, L.; Pacquet, J.Y.; Robert, E.; Saint-Laurent, M.G.; Seron, D.; Sortais, P.; Villari, A.C.C. [Grand Accelerateur National d' Ions Lourds (GANIL), 14 - Caen (France); Angelique, J.C.; Orr, N.A. [Laboratoire de Physique Corpusculaire (CNRS/IN2P3), ISMRA, 14 - Caen (France); Blank, B. [CENBG, Centre d' Etudes Nucleaires de Bordeaux Gradignan, 33 (France); Clapier, F.; Ducourtieux, M.; Kandri-Rody, S.; Obert, J.; Pauwels, N.; Proust, J.; Putaux, J.C. [Institut de Physique Nucleaire, (IN2P3/CNRS) 91 - Orsay (France); Lepine, A. [IFUSP, C.P., Sao Paulo (Brazil)

    1999-07-01

    In order to define the future intensity and reliability of the on line radioactive beams for the SPIRAL project, an intense activity of research and development is being done around the target and the ion source problems. The main instrument for this purpose is the isotopic separator SIRa (Separateur d'Ions Radioactifs) installed in the D2 experimental cave at GANIL. One of the research axis is the production of multicharged radioactive condensable ions. In this aim, we have built a new compact ECR ion source, SHyPIE (Source Hybride pour la Production d'Ions Exotiques), whose original magnetic configuration is under patent since 1997. This new magnetic structure allows to place an internal production target very close to the plasma, while avoiding radiation damages of the sensitive permanent magnets. A series of on line experiments have been done, using SHyPIE with several internal target systems, and around thirty species of condensable and noble gases radioactive multicharged ion beams have been produced. The behaviour of the plasma in a close geometry with the production target has been studied. (authors)

  16. Effect of swift heavy ion (SHI) irradiation on transparent conducting oxide electrodes for dye-sensitized solar cell applications

    Science.gov (United States)

    Singh, Hemant Kr.; Avasthi, D. K.; Aggarwal, Shruti

    2015-06-01

    Transparent conducting oxides (TCOs) are used as electrodes in dye-sensitized solar cells (DSSCs) because of their properties such as high transmittance and low resistivity. In the present work, the effects of swift heavy ion (SHI) irradiation on various types of TCOs are presented. The objective of this study is to investigate the effect of SHI on TCOs. For the present study, three different types of TCOs are considered, namely, (a) FTO (fluorine-doped tin oxide, SnO2:F) on a Nippon glass substrate, (b) ITO (indium tin oxide, In2O3:Sn) coated on polyethylene terephthalate (PET) on a Corning glass substrate, and (c) ITO on a Corning glass substrate. These films are irradiated with 120 MeV Ag+9 ions at fluences ranging from 3.0 × 1011 ions/cm2 to 3.0 × 1013 ions/cm2. The structural, morphological, optical and electrical properties are studied via X-ray diffraction (XRD), atomic force microscopy (AFM), UV-Vis absorption spectroscopy and four-probe resistivity measurements, respectively. The ITO-PET electrode is found to exhibit superior conductivity and transmittance properties in comparison with the others after irradiation and, therefore, to be the most suitable for solar cell applications.

  17. Identification and Correction of Shi Naian’ s Epitaph%《施耐庵墓志》辨正

    Institute of Scientific and Technical Information of China (English)

    陈传坤

    2011-01-01

    Shi Naian’ s Epitaph written by Wang Daosheng of the Yuan dynasty was recorded in Xinghua County Continued History compiled in the period of Republic of China.This epitaph provides exact evidences for the biography of Shi Naian,the author of Water Margin.However,because the epitaph appeared too late from the times of its writer,there have been various doubts about its authenticity.In this paper,the author discriminates the doubts by etymological study and ancient institutions and facts.It is concluded that the text of the epitaph is not contrary to other literary material,thus proving that the epitaph is believable.%民国间修撰之《兴化县续志》载录了元人王道生所撰之《施耐庵墓志》,该墓志提供了《水浒》作者施耐庵行状之确切证据。但是,因该墓志面世时间远离作者的时代,由此质疑之声不绝如缕。本文就反对者提议的"质疑"点一一辨析,借助语源和典章故实等佐证该墓志文本并无违背其他文献史料之处,反证《施耐庵墓志》之可信无疑。

  18. SHI induced effects on the electrical and optical properties of HfO2 thin films deposited by RF sputtering

    Science.gov (United States)

    Manikanthababu, N.; Dhanunjaya, M.; Nageswara Rao, S. V. S.; Pathak, A. P.

    2016-07-01

    The continuous downscaling of Metal Oxide Semiconductor (MOS) devices has reached a limit with SiO2 as a gate dielectric material. Introducing high-k dielectric materials as a replacement for the conservative SiO2 is the only alternative to reduce the leakage current. HfO2 is a reliable and an impending material for the wide usage as a gate dielectric in semiconductor industry. HfO2 thin films were synthesized by RF sputtering technique. Here, we present a study of Swift Heavy Ion (SHI) irradiation with100 MeV Ag ions for studying the optical properties as well as 80 MeV Ni ions for studying the electrical properties of HfO2/Si thin films. Rutherford Backscattering Spectrometry (RBS), Field Emission Scanning Electron Microscope (FESEM), energy-dispersive X-ray spectroscopy (EDS), profilometer and I-V (leakage current) measurements have been employed to study the SHI induced effects on both the structural, electrical and optical properties.

  19. Chemical Constituents from the Seeds of Ziziphus jujuba var. Spinosa (Bunge) Hu

    Institute of Scientific and Technical Information of China (English)

    Li-Mei LI; Xun LIAO; Shu-Lin PENG; Li-Sheng DING

    2005-01-01

    To search for new and bioactive minor components from traditional Chinese medicines, a new compound, named jujuphenoside (1), was isolated from the seeds of Ziziphusjujuba var. spinosa (Bunge)Hu. The structure of jujuphenoside was elucidated by spectral and chemical methods, particularly twodimensional nuclear magnetic resonance analysis. Together with the new compound, 22 known compounds were also isolated and identified from the seeds of Z. jujuba var. spinosa, among which, epiceanothic acid (2) was first obtained from natural resources, whereas compounds 7-16 were first obtained from this plant.

  20. Opsoclonus-myoclonus and anti-Hu positive limbic encephalitis in a patient with neuroblastoma.

    Science.gov (United States)

    Morales La Madrid, Andres; Rubin, Charles M; Kohrman, Michael; Pytel, Peter; Cohn, Susan L

    2012-03-01

    Opsoclonus-myoclonus syndrome (OMS) is seen in 2-3% of children with neuroblastoma and is believed to be caused by an autoimmune process elicited by the tumor. Although long-term neurologic sequelae are common in children with OMS, limbic encephalitis has not previously been reported. We report a child who developed limbic encephalitis associated with anti-Hu antibodies, 6 years after her initial diagnosis of neuroblastoma and OMS. This case demonstrates that patients with neuroblastoma and OMS are at risk for developing new paraneoplastic symptoms years after their original diagnosis and emphasizes the need for careful long-term follow-up.

  1. Micología Lista de Huéspedes Colombianos

    Directory of Open Access Journals (Sweden)

    Obregon Botero Rafael

    1964-12-01

    Full Text Available El presente trabajo es una lista de hongos encontrados en Colombia. Está ordenada por Familias del huésped y cada nombre científico del hongo va seguido por la mención de los lugares donde fue encontrado así como la altura de tales localidades. Luego precede un número que corresponde a la colección existente en el Ministerio de Agricultura de Colombia. No pretendo sino iniciar la labor que han de completar especialistas mejor informados y espero que sea de alguna utilidad para los que se dedican a esta rama de las Ciencias Naturales.

  2. Hybrid Semiloof elements for plates and shells based upon a modified Hu-Washizu principle

    Science.gov (United States)

    Pian, T. H. H.; Sumihara, K.

    1984-01-01

    Hybrid SemiLoof elements for plates and shells are developed based upon modified Hu-Washizu principle. In the new version of the assumed stress hybrid formulation the equilibrium equations are satisfied through the introduction of internal displacement parameters as Lagrange multipliers. The inversion of the resulting H-matrices is simplified particularly when the stresses are expressed in terms of natural coordinates. A 24-DOF triangular element and a 32-DOF quadrilateral element based on shallow shell theory are derived and evaluated.

  3. HuLiS, a program to teach mesomerism and more

    Science.gov (United States)

    Carissan, Yannick; Goudard, Nicolas; Hagebaum-Reignier, Denis; Humbel, Stéphane

    2016-08-01

    The HuLiS program is presented as a tool to decompose a delocalized wave function as a linear combination of localized electronic structures. The principles of the energy based HL-CI and the overlap based schemes HL-P are developed. The results obtained for a set of 10 relevant organic molecules are shown to compare very well with high level quantum chemistry calculations. A trust factort is introduced and its use is shown on the allyl radical case where symmetry must be taken into account.

  4. MicroRNA-16 Modulates HuR Regulation of Cyclin E1 in Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Xun Guo

    2015-03-01

    Full Text Available RNA binding protein (RBPs and microRNAs (miRNAs or miRs are post-transcriptional regulators of gene expression that are implicated in development of cancers. Although their individual roles have been studied, the crosstalk between RBPs and miRNAs is under intense investigation. Here, we show that in breast cancer cells, cyclin E1 upregulation by the RBP HuR is through specific binding to regions in the cyclin E1 mRNA 3' untranslated region (3'UTR containing U-rich elements. Similarly, miR-16 represses cyclin E1, dependent on its cognate binding sites in the cyclin E1 3'UTR. Evidence in the literature indicates that HuR can regulate miRNA expression and recruit or dissociate RNA-induced silencing complexes (RISC. Despite this, miR-16 and HuR do not affect the other’s expression level or binding to the cyclin E1 3'UTR. While HuR overexpression partially blocks miR-16 repression of a reporter mRNA containing the cyclin E1 3'UTR, it does not block miR-16 repression of endogenous cyclin E1 mRNA. In contrast, miR-16 blocks HuR-mediated upregulation of cyclin E1. Overall our results suggest that miR-16 can override HuR upregulation of cyclin E1 without affecting HuR expression or association with the cyclin E1 mRNA.

  5. 可在鱼体内表达hu-IFN-α的基因重组构建及转化%Construction of a Fish Expression hu-IFN-α Recombinant Gene

    Institute of Scientific and Technical Information of China (English)

    张学文; 章怀云; 符少辉; 陈韬; 陈立祥; 肖调义

    2001-01-01

    通过分子重组,将人α-干扰素(hu-IFN-α)基因编码序列克隆到鲤鱼β-肌动蛋白基因启动子下游,构建成能在鱼体内组成型表达hu-IFN-α的基因重组分子。经限制性酶切分析、Southern杂交和序列测定,证明重组分子构建的正确性。采用显微注射法将这一重组基因转化草鱼受精卵,获得了表达人α-干扰素的转基因草鱼。%The coding sequence of hu-IFN-α gene is cloned under the control of Carp β-actin gene promoter. Thus a fish constantly expression of hu-IFN-α gene recombinant is constructed. Southern blot and sequencing results show that the recombinant molecule is correctly constructed. The recombinant gene is transferred into fertilized zygotes of grass carp via microinjection. And hu-IFN-α is detected out in 20% of transgenic fish by ELISA.

  6. 新型高效原油破乳剂SHY405的研制及其在小河集输站的应用%Development of Demulsifying Agent SHY405 and Application in Jingbian Plant Oil Extraction

    Institute of Scientific and Technical Information of China (English)

    任海晶; 李俊华; 刘彦锋; 刘世川; 李俊莉; 李移乐; 陈强

    2011-01-01

    The crude oil(water content3 %) extracted from Xiaohe gathering station in Jingbian Plant oil extraction plant of Yanchang petroleum group were mainly transported by tank car or in pipelines.There were several defects for the exploitation of the crude oil.For example,the exploitation time was often too long,the gas content in the crude oil and the low water were always too low.And also,the temperature of the field operation changed frequently.Additionally,the demulsifying agent used in the process could not work efficiently.Some defects,such as incomplete dehydration,strong demand for the amount,and needs of low temperature were often appeared in the utilization of the Demulsifying agent.In order to overcome the defects of the traditional Demulsifying agent,herein,we developed a new type of demulsifying agent,named SHY405.The test result indicated that the average moisture content in purify crude oil after field test processing of 9 times was 0.09 %,such a result was obtained at a precipitate temperature of 40 ℃ and the addition amount of 251~289 mg/L.The residual moisture content was accorded with the stranded described in the mineral oil technical conditions,which provide an index of 0.5 %.Our test results suggested that demulsifying agent SHY405 could satisfied the requirements in the field of production.%靖边采油厂小河集输站现用破乳剂存在脱水率低、用量大、含水高、脱水时间长等问题。文章研制的一种新型SHY405原油破乳剂,在加量251~289 mg/L时,沉降温度40℃左右的条件下,9次现场试验处理后的净化油平均含水率为0.09%(ω—质量分数),小于出矿原油技术条件(SY 7513-1988)标准要求的不大于0.50%的指标,能够满足现场生产使用要求。

  7. Genetic determination of the enhanced drought resistance of rice maintainer HuHan2B by pedigree breeding.

    Science.gov (United States)

    Wei, Haibin; Feng, Fangjun; Lou, Qiaojun; Xia, Hui; Ma, Xiaosong; Liu, Yunhua; Xu, Kai; Yu, Xinqiao; Mei, Hanwei; Luo, Lijun

    2016-11-17

    The ongoing deficit of fresh water resource in rice growing regions has made the selection of water-saving and drought-resistance rice (WDR) a crucial factor in developing sustainable cultivation. HuHan2B, a new japonica maintainer for WDR breeding, had the same yield potential as recurrent parent HanFengB but showed improved drought resistance in fields. We investigated the genomic content accumulation and candidate genes passed from parent to offspring using the genomic and transcriptomic approaches. The genomic constitution indicated that the genetic similarity was 84% between HuHan2B and HanFengB; additionally, 7,256 genes with specific alleles were inherited by HuHan2B from parents other than HanFengB. The differentially expressed genes (DEGs) under drought stress showed that biological function was significantly enriched for transcript regulation in HuHan2B, while the oxidation-reduction process was primarily enriched in HanFengB. Furthermore, 36 DEGs with specific inherited alleles in HuHan2B were almost involved in the regulatory network of TFs and target genes. These findings suggested that major-effect genes were congregated and transformed into offspring in manner of interacting network by breeding. Thus, exploiting the potential biological function of allelic-influencing DEGs would be of great importance for improving selection efficiency and the overall genetic gain of multiple complex traits.

  8. HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes

    Directory of Open Access Journals (Sweden)

    Apostolia Guialis

    2013-10-01

    Full Text Available Post-transcriptional regulatory networks are dependent on the interplay of many RNA-binding proteins having a major role in mRNA processing events in mammals. We have been interested in the concerted action of the two RNA-binding proteins hnRNP A1 and HuR, both stable components of immunoselected hnRNP complexes and having a major nuclear localization. Specifically, we present here the application of the RNA-immunoprecipitation (RIP-Chip technology to identify a population of nuclear transcripts associated with hnRNP A1-RNPs as isolated from the nuclear extract of either HuR WT or HuR-depleted (KO mouse embryonic fibroblast (MEF cells. The outcome of this analysis was a list of target genes regulated via HuR for their association (either increased or reduced with the nuclear hnRNP A1-RNP complexes. Real time PCR analysis was applied to validate a selected number of nuclear mRNA transcripts, as well as to identify pre-spliced transcripts (in addition to their mature mRNA counterpart within the isolated nuclear hnRNP A1-RNPs. The differentially enriched mRNAs were found to belong to GO categories relevant to biological processes anticipated for hnRNP A1 and HuR (such as transport, transcription, translation, apoptosis and cell cycle indicating their concerted function in mRNA metabolism.

  9. Effects of rHu-EPO on Myocyte Apoptosis and Cardiac Function Follow-ing Acute Myocardial Infarction in Rats

    Institute of Scientific and Technical Information of China (English)

    YE Liang; DU Xinling; XIA Jiahong; JIANG Ping

    2005-01-01

    The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infarction in rats were studied. Thirty-two rats were divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI+ EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery. rHu-EPO was administered i. p. in MI+EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI+EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2, bax) and TUNEL dyeing. The results showed that hemodynamic function in MI+ EPO group was much better than in MI group.The number of the cells positive for bax and TUNEL in MI+EPO group was less than that in MI group. The number of the cells positive for bcl-2 in MI+EPO group was more than that in MI group. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function.

  10. The Use of Soluble Transferrin Receptor in the Detection of rHuEPO Abuse in Sports

    Directory of Open Access Journals (Sweden)

    Lorenzo Gordon

    2010-02-01

    Full Text Available Erythropoietin (EPO increases the number of circulating erythrocytes and muscle oxygenation. The recombinant forms of EPO have indiscriminately been used by athletes, mainly in endurance sports to increase their erythrocytes concentration, thus generating a better delivery of oxygen to the muscle tissue. The administration of recombinant human erythropoietin (rHuEPO except for therapeutic use was prohibited by the International Olympic Committee (IOC and its unauthorized use considered as doping. In the last few years, a number of studies using parameters indicative of accelerated erythropoiesis have investigated a number of indirect methods for the detection of rHuEPO abuse. No single indirect marker has been found that can satisfactorily demonstrated rHuEPO misuse. Soluble transferrin receptor (sTfR is a new marker of iron status and erythropoietic activity. It has been included in multivariable blood testing models for the detection of performance enhancing EPO abuse in sports. Indirect markers of altered erythropoiesis give reliable evidence of current or discontinued rHuEPO usage. This review describes the physical, biological and pharmacokinetic properties of endogenous EPO and its recombinant form. It also discusses the available strategies for the detection of rHuEPO abuse in sports, involving the use of sTfR concentration directly or in mathematical multivariate models.

  11. Potential Risk of Hybridization in Ex Situ Collections of Two Endangered Species of Sinojackia Hu (Styracaceae)

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Spontaneous hybridization in ex situ facilities can undermine the genetic integrity of ex situ collections and potentially contaminate open-pollinated seeds or seedlings destined for the reintroduction of endangered plant species into the wild. In the present study, the potential risk of hybridization between two endangered Chinese endemic species, namely Sinojackia xylocarpa Hu and S. rehderiana Hu, which are naturally allopatric species but were conserved ex situ in Wuhan Botanical Garden (WBG), Wuhan, China, were investigated over three consecutive years from 2003 to 2005. The entire overlapping flowering period of the two species was 14-20 d and the two species shared the same pollinator insects during the entire flowering season in WBG. The floral isolation between the two species was not an issue in the ex situ collection at WBG. The results suggest an opportunity for pollen transfer between species and a potential risk of genetic introgression and loss of genetic identity of open-pollinated seeds produced in the ex situ collection of these two endangered species. An artificial reciprocal cross between S. xylocarpa and S. rehderiana confirmed that the two congener species could readily set seeds, indicating no post-pollination barriers to hybridization and the importance of spatial isolation as a barrier to inter-specific crossing. Therefore, to manage these crossable species with overlapping flowering times and shared pollination vectors in ex situ facilities, spatial isolation should be carefully considered to minimize the possibility of spontaneous hybridization.

  12. Combining wavelets transform and Hu moments with self-organizing maps for medical image categorization

    Science.gov (United States)

    Silva, Leandro A.; Del-Moral-Hernandez, Emilio; Moreno, Ramon A.; Furuie, Sérgio S.

    2011-10-01

    Images are fundamental sources of information in modern medicine. The images stored in a database and divided in categories are an important step for image retrieval. For an automatic categorization process, detailed analysis is done regarding image representation and generalization method. The baseline method for this process, in the medical image context, is using thumbnails and K-nearest neighbor (KNN), which is easily implemented and has had satisfactory results in literature. This work addresses an alternative method for automatic categorization, which jointly uses discrete wavelet transform with Hu's moments for image representation and self-organizing maps (SOM) neural networks combined with the KNN classifier (SOM-KNN), for medical image categorization. Furthermore, extensive experiments are conducted, to define the best wavelet family and to select the best coefficients set, to consider the remaining wavelet coefficients set (not selected as the best ones) through their Hu's moments, and to carry out a contrastive study with other successful approaches for categorization. The categorization result from a database with 10,000 images in 116 categories yielded 81.8% of correct rate, which is much better than the 67.9% obtained by the baseline method; and the time consumed in classification processing with SOM-KNN is 100 times shorter than KNN.

  13. Hu1-2: a metal-poor bipolar planetary nebula with fast collimated outflows

    CERN Document Server

    Fang, Xuan; Miranda, Luis F; Riera, Angels; Velazquez, Pablo F; Raga, Alejandro C

    2015-01-01

    We present narrow-band optical and near-IR imaging and optical long-slit spectroscopic observations of Hu1-2, a Galactic planetary nebula (PN) with a pair of [N II]-bright, fast-moving (> 340 km/s) bipolar knots. Intermediate-dispersion spectra are used to derive physical conditions and abundances across the nebula, and high-dispersion spectra to study the spatio-kinematical structure. Generally Hu1-2 has high He/H (~0.14) and N/O ratios (~0.9), typical of Type I PNe. On the other hand, its abundances of O, Ne, S, and Ar are low as compared with the average abundances of Galactic bulge and disc PNe. The position-velocity maps can be generally described as an hour-glass shaped nebula with bipolar expansion, although the morphology and kinematics of the innermost regions cannot be satisfactorily explained with a simple, tilted equatorial torus. The spatio-kinematical study confines the inclination angle of its major axis to be within 10 degrees of the plane of sky. As in the irradiated bow-shocks of IC4634 and ...

  14. Revisiting the proposed planetary system orbiting the eclipsing polar HU Aquarii

    CERN Document Server

    Wittenmyer, Robert A; Marshall, J P; Butters, O W; Tinney, C G

    2011-01-01

    It has recently been proposed, on the basis of eclipse-timing data, that the eclipsing polar cataclysmic variable HU Aquarii is host to at least two giant planets. However, that result has been called into question based upon the dynamical stability of the proposed planets. In this work, we present a detailed re-analysis of all eclipse timing data available for the HU Aquarii system, making use of standard techniques used to fit orbits to radial-velocity data. We find that the eclipse timings can be used to obtain a two-planet solution that does not require the presence of additional bodies within the system. We then perform a highly detailed dynamical analysis of the proposed planetary system. We show that the improved orbital parameters we have derived correspond to planets that are dynamically unstable on unfeasibly short timescales (of order 10^4 years or less). Given these results, we discuss briefly how the observed signal might in fact be the result of the intrinsic properties of the eclipsing polar, r...

  15. ReHuNa- La Red por la Humanización del Parto y Nacimiento

    Directory of Open Access Journals (Sweden)

    Daphne RATTNER

    2010-12-01

    Full Text Available La ReHuNa - Red por la Humanización del Parto y Nacimiento - es una organización de la sociedad civil que actúa desde 1993 en forma de red de asociados en el Brasil. Su principal objetivo es la divulgación de la asistencia y cuidados perinatales con base en evidencias científicas y de acuerdo a las recomendaciones de la Organización Mundial de la Salud. Esta red tuvo un papel fundamental en la estructura de un movimiento que hoy se denomina “humanización de la asistencia al parto y nacimiento”, que pretende disminuir las intervenciones innecesarias y promover cuidados al proceso del embarazo-parto-nacimiento-lactancia comprendido como un proceso natural y fisiológico basado en conocimientos científicos. Este artículo relata la historia de la ReHuNa y sus principales realizaciones y señala sus caminos futuros.

  16. The impact of the vaccination program for hemorrhagic fever with renal syndrome in Hu County, China.

    Science.gov (United States)

    Xiao, Dan; Wu, Kejian; Tan, Xin; Yan, Tiecheng; Li, Haitao; Yan, Yongping

    2014-02-03

    The incidence of hemorrhagic fever with renal syndrome (HFRS) in Hu County ranked third of all counties in China in 2010. Although this county has provided a HFRS vaccination program freely since 1994, the impact of HFRS remains quite substantial. In order to continue the vaccination program effectively and control HFRS, a detailed understanding of the effect of the vaccination program should be undertaken. The Cochran-Armitage trend test was employed to examine the temporal trends of HFRS incidences, mortality rate and vaccination compliance. Temporal cluster analysis was performed to detect time periods of high HFRS risk. Cross correlation analysis was conducted to detect the correlation between HFRS incidence and vaccination compliance. Wavelet analysis was employed to detect the shift of the periodicity of HFRS. Between 1971 and 2011, the HFRS incidence and mortality rate ranged from 9.53/100,000 to 300.57/100,000 and 0 to 24.91/100,000, respectively, with a fluctuating but distinctly declining trend (incidence: Z=-34.38, P1994. In conclusion, the increase in vaccination compliance may play an important role in HFRS control and prevention in Hu County, China.

  17. The SCID-hu mouse as a model for HIV-1 infection.

    Science.gov (United States)

    Aldrovandi, G M; Feuer, G; Gao, L; Jamieson, B; Kristeva, M; Chen, I S; Zack, J A

    1993-06-24

    During normal fetal ontogeny, one of the first organs to harbour CD4-positive cells is the thymus. This organ could therefore be one of the earliest targets infected by human immunodeficiency virus type 1 (HIV-1) in utero. HIV-1-infected cells and pathological abnormalities of the thymus have been seen in HIV-1-infected adults and children, and in some fetuses aborted from infected women. Studies of HIV-1 pathogenesis have been hampered by lack of a suitable animal model system. Here we use the SCID-hu mouse as a model to investigate the effect of virus infection on human tissue. The mouse is homozygous for the severe combined immunodeficiency (SCID) defect. The model is constructed by implanting human fetal liver and thymus under the mouse kidney capsule. A conjoint human organ develops, which allows normal maturation of human thymocytes. After direct inoculation of HIV-1 into these implants, we observed severe depletion of human CD4-bearing cells within a few weeks of infection. This correlated with increasing virus load in the implants. Thus the SCID-hu mouse may be a useful in vivo system for the study of HIV-1-induced pathology.

  18. RNA-binding protein HuD reduces triglyceride production in pancreatic β cells by enhancing the expression of insulin-induced gene 1.

    Science.gov (United States)

    Kim, Chongtae; Lee, Heejin; Kang, Hoin; Shin, Jung Jae; Tak, Hyosun; Kim, Wook; Gorospe, Myriam; Lee, Eun Kyung

    2016-04-01

    Although triglyceride (TG) accumulation in the pancreas leads to β-cell dysfunction and raises the chance to develop metabolic disorders such as type 2 diabetes (T2DM), the molecular mechanisms whereby intracellular TG levels are regulated in pancreatic β cells have not been fully elucidated. Here, we present evidence that the RNA-binding protein HuD regulates TG production in pancreatic β cells. Mouse insulinoma βTC6 cells stably expressing a small hairpin RNA targeting HuD (shHuD) (βTC6-shHuD) contained higher TG levels compared to control cells. Moreover, downregulation of HuD resulted in a decrease in insulin-induced gene 1 (INSIG1) levels but not in the levels of sterol regulatory element-binding protein 1c (SREBP1c), a key transcription factor for lipid production. We identified Insig1 mRNA as a direct target of HuD by using ribonucleoprotein immunoprecipitation (RIP) and biotin pulldown analyses. By associating with the 3'-untranslated region (3'UTR) of Insig1 mRNA, HuD promoted INSIG1 translation; accordingly, HuD downregulation reduced while ectopic HuD expression increased INSIG1 levels. We further observed that HuD downregulation facilitated the nuclear localization of SREBP1c, thereby increasing the transcriptional activity of SREBP1c and the expression of target genes involved in lipogenesis; likewise, we observed lower INSIG1 levels in the pancreatic islets of HuD-null mice. Taken together, our results indicate that HuD functions as a novel repressor of lipid synthesis in pancreatic β cells.

  19. HuR-targeted nanotherapy in combination with AMD3100 suppresses CXCR4 expression, cell growth, migration, and invasion in lung cancer

    Science.gov (United States)

    Muralidharan, Ranganayaki; Panneerselvam, Janani; Chen, Allshine; Zhao, Yan Daniel; Munshi, Anupama; Ramesh, Rajagopal

    2015-01-01

    The CXCR4 chemokine receptor plays an important role in cancer cell metastasis. The CXCR4 antagonist, AMD3100, has limited efficacy in controlling metastasis. HuR, an RNA-binding protein, regulates CXCR4 in cancer cells. We therefore investigated whether targeting HuR using a siRNA-based nanoparticle plus AMD3100 would suppress CXCR4 and inhibit lung cancer metastasis. We treated human H1299 lung cancer cell with HuR-specific siRNA contained in a folate-targeted lipid nanoparticle (HuR-FNP) plus AMD3100, and compared this with AMD3100 alone, HuR-FNP alone and no treatment. HuR-FNP plus AMD3100 treatment produced a G1 phase cell-cycle arrest and reduced cell viability above and beyond the effects of AMD3100 alone. HuR and CXCR4 mRNA and protein expression levels were markedly reduced in all treatment groups. Phosphorylated (p) AKTS473 protein was also reduced. P27 protein expression increased with HuR-FNP and combination treatment. Promoter-based reporter studies showed that the combination inhibited CXCR4 promoter activity more than did either treatment alone. Cell migration and invasion was significantly reduced with all treatment; the combination provided the most inhibition. Reduced matrix metalloprotease (MMP) -2 and -9 expression was associated with reduced invasion in all treatment groups. Thus, we found that combined HuR and CXCR4 targeting effectively controlled lung cancer metastasis. PMID:26494555

  20. HuR-targeted nanotherapy in combination with AMD3100 suppresses CXCR4 expression, cell growth, migration and invasion in lung cancer.

    Science.gov (United States)

    Muralidharan, R; Panneerselvam, J; Chen, A; Zhao, Y D; Munshi, A; Ramesh, R

    2015-12-01

    The CXCR4 chemokine receptor has an important role in cancer cell metastasis. The CXCR4 antagonist, AMD3100, has limited efficacy in controlling metastasis. HuR, an RNA-binding protein, regulates CXCR4 in cancer cells. We therefore investigated whether targeting HuR using a siRNA-based nanoparticle plus AMD3100 would suppress CXCR4 and inhibit lung cancer metastasis. We treated human H1299 lung cancer cells with HuR-specific siRNA contained in a folate-targeted lipid nanoparticle (HuR-FNP) plus AMD3100, and compared this with AMD3100 alone, HuR-FNP alone and no treatment. HuR-FNP plus AMD3100 treatment produced a G1 phase cell cycle arrest and reduced cell viability above and beyond the effects of AMD3100 alone. HuR and CXCR4 mRNA and protein expression levels were markedly reduced in all treatment groups. Phosphorylated (p) AKT(S473) protein was also reduced. P27 protein expression increased with HuR-FNP and combination treatment. Promoter-based reporter studies showed that the combination inhibited CXCR4 promoter activity more than did either treatment alone. Cell migration and invasion was significantly reduced with all treatments; the combination provided the most inhibition. Reduced matrix metalloprotease (MMP)-2 and -9 expression was associated with reduced invasion in all treatment groups. Thus, we found that combined HuR and CXCR4 targeting effectively controlled lung cancer metastasis.

  1. Analysis of "Mengyang", a Concept from Shi Tao's Commentaries on Painting%《石涛画语录》"蒙养"解析

    Institute of Scientific and Technical Information of China (English)

    令狐小

    2015-01-01

    石涛作为清初的僧人画家,以其独具别格的画风和画论被人所熟知."蒙养"是他画论中所提到的重要概念之一.这个概念并不属于石涛原创,是从中国古代《周易》中继承而来,然而在《石涛画语录》中,石涛结合自己对生活与艺术的理解,赋予"蒙养"新的含义,对画家深刻理解艺术与生活、修养与创作之间的关系深具启迪作用.%Shi Tao, as a monk painter in the early Qing Dynasty, was known for his unique painting style and theory. "Mengyang" is one of the most important concepts he referred to in his painting theory. This concept was not created by Shi Tao, but came fromThe Book of Changes. However, inShi Tao's Commentaries on Painting, in connection with his own understanding of life and art, Shi Tao endows "mengyang" with a new meaning, which deeply enlightens painters in their understanding of the relationship between art and life, accomplishment and creation.

  2. An Analysis of Tree English Translations of“To the Tune of‘Water Dragon Song1’”by Su Shi

    Institute of Scientific and Technical Information of China (English)

    谭敏

    2014-01-01

    Su Shi is known for his poems of haofang style as well as wanyue style. The author takes a comparative reading of three English renderings of“Water Dragon Song”;both achievements and problems in each version are discussed regarding imag-ery, meaning, poet’s sentiments and poetic atmosphere, for the purpose of working out a better rendering.

  3. A Polarized Light Microscopic Study to Comparatively evaluate Four Remineralizing Agents on Enamel viz CPP-ACPF, ReminPro, SHY-NM and Colgate Strong Teeth.

    Science.gov (United States)

    Rajan, Reshma; Krishnan, Ramesh; Bhaskaran, Bibin; Kumar, Suresh V

    2015-01-01

    To compare and evaluate the remineralizing potential of four commercially available products namely SHY-NM, GC Tooth Mousse Plus, ReminPro and Colgate strong teeth on demineralized human teeth. The study included 50 extracted premolars having 3 × 3 mm window prepared on the middle third of the tooth, which was then subjected to demineralization for 48 hours at 37°C. Teeth were randomly selected and grouped into five study groups of 10 teeth in each. Each group was treated with respective remineralizing agent and sectioned using hard-tissue microtome. Each section obtained was visualized under polarized light microscope and analyzed using Image J software. The statistically evaluated results revealed that SHY-NM has the most remineralizing potential followed by ReminPro, GC Tooth Mousse Plus and fluoridated toothpaste. Based on the study, the SHY-NM was superior to the GC Tooth Mousse Plus, ReminPro and Colgate strong teeth on demineralized human teeth. How to cite this article: Rajan R, Krishnan R, Bhaskaran B, Kumar SV. A Polarized Light Microscopic Study to Comparatively evaluate Four Remineralizing Agents on Enamel viz CPP-ACPF, ReminPro, SHY-NM and Colgate Strong Teeth. Int J Clin Pediatr Dent 2015;8(1):42-47.

  4. Getting to "No": Locating Critical Peace Education within Resistance and Anti-Oppression Pedagogy at a Shi'a Islamic School in Lebanon

    Science.gov (United States)

    Zakharia, Zeena

    2017-01-01

    This paper critically engages observations from a school that was aligned with a resistance movement in Lebanon during a post-war period of sustained political violence (2006-2007). Focusing on the pedagogical practices at one community-centered and community-led Shi'a Islamic urban school, the paper draws on extensive ethnographic data to…

  5. Overexpression of the AtSHI gene in poinsettia, Euphorbia pulcherrima, results in compact plants

    DEFF Research Database (Denmark)

    Islam, M. Ashraful; Lütken, Henrik Vlk; Haugslien, Sissel;

    2013-01-01

    with desirable height and reduce the utilization of growth retardants, the Arabidopsis SHORT INTERNODE (AtSHI) gene controlled by the cauliflower mosaic virus 35S promoter was introduced into poinsettia by Agrobacterium-mediated transformation. Three independent transgenic lines were produced and stable...

  6. Expression of ARE-binding proteins AUF1 and HuR in follicular adenoma and carcinoma of thyroid gland.

    Science.gov (United States)

    Trojanowicz, B; Sekulla, C; Dralle, H; Hoang-Vu, C

    2016-01-01

    Both adenylate-uridylate rich elements binding proteins AUF1 and HuR may participate in thyroid carcinoma progression. In this study we investigated the expression of both factors on a protein level with a special focus on follicular adenoma and follicular thyroid carcinoma. By employment of immunofluorescence and western blot on 68 thyroid tissues including 7 goiter, 16 follicular adenoma (4 adenomatous hyperplasia), 19 follicular thyroid carcinomas, 13 papillary thyroid carcinomas and 14 undifferentiated thyroid carcinomas we investigated protein expression of AUF1 and HuR. In addition to previous results we demonstrated that AUF1 and HuR are significantly up-regulated in carcinoma tissues as compared with follicular adenoma or goiter tissues. Furthermore, by evaluation of AUF1 or HuR expression, or combination of both proteins on total tissue lysates, we were able to demonstrate a significant difference between follicular adenoma and follicular thyroid carcinoma. Overexpression of AUF1 and HuR is a common finding observed in thyroid malignancy. Analysis of the tissues obtained by surgical resection as demonstrated in this study is comparable to a fine needle aspiration and in combination with AUF1/HuR immuno-analysis may support the conventional immunohistological investigations. The promising results of this study were performed on relatively small collective, but justify future development of a quick thyroid diagnostic test on larger cohort of the patients, especially for thyroid samples which are inadequate for histological examinations.

  7. SHiP: a new facility to search for heavy neutrinos and study ντ properties

    Science.gov (United States)

    De Serio, M.; SHiP Collaboration

    2016-05-01

    SHiP (Search for Hidden Particles) is a newly designed fixed target facility, proposed at the CERN SPS accelerator, with the aim of complementing searches for New Physics at LHC by searching for light long-lived exotic particles with masses below a few GeV/c2. The sensitivity to Heavy Neutrinos will allow for the first time probing a region of the parameter space where Baryogenesis and active neutrino masses and oscillation could also be explained. A dedicated detector, based on OPERA-like bricks, will provide the first observation of the tau anti-neutrino. Moreover, ντ and ν¯τ cross-sections will be measured with a statistics 1000 times larger than currently available data and will allow extracting the F4 and F5 structure functions, never measured so far. Charm physics studies will be performed with significantly improved accuracy with respect to past experiments.

  8. 张学峰:编著《中国墓葬史》%Xuefeng Zhang, Zhongguo muzang shi

    Institute of Scientific and Technical Information of China (English)

    邓玮光

    2010-01-01

    @@ Since the oracle bones were discovered in the 19th century, these archaeological texts have received the attention of the historical world. However, the number of texts excavated is relatively small; thus, the question of how to use archeological materials that do not have text is a problem that archeologists and historians must face. Today this problem has been limited to the textual criticism of these relics.Thus, the recent publication of Zhongguo muzang shi (History of Chinese tombs)is especially meaningful. The editor of the volume is an archeologist and the book's intended audience is the broader historical community. The way the materials are presented by archeologists and the thinking behind these is the key to the success of this book.

  9. 苏轼的诗歌及其时代%On Su Shi's Poetry and his Times

    Institute of Scientific and Technical Information of China (English)

    顾易生

    2001-01-01

    Su Shi was the most important writer in South Song Dynasty. Singin g fo r democracy and freedom, his poetry showed distinctive democratic consciousness which stood on the forward of the contemporary times, moreover developed the art istry of poetry. The realism and romanticism in his poems stood for a “new leve l" of Chinese classical literary history.%苏轼是北宋时期最大的文学家。苏诗闪耀着某种民主自由精神的光芒,表现出鲜明的主体意识,也发展了诗歌的艺术手法。其思想站在“时代前沿”,其诗歌中的现实主义与浪漫主义,创造了中国古代文学史的“新高度”。

  10. Antonio Rodríguez Huéscar y José Ortega y Gasset: Tres cartas

    OpenAIRE

    Esteban Enguita, José Emilio; Lasaga Medina, José

    2015-01-01

    El objetivo principal de este artículo es comentar las cartas que se enviaron Antonio Rodríguez Huéscar y José Ortega y Gasset entre 1942 y 1943. Dichas cartas se incluyen en el artículo. The principal aim of this paper is to comment on the letters which Antonio Rodríguez Huéscar and José Ortega y Gasset sent each other between 1942 and 1943. The above said letters are included in the paper

  11. The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA

    OpenAIRE

    Lopez de Silanes, I.; Gorospe, M.; Taniguchi, H; Abdelmohsen, K; Srikantan, S.; Alaminos, M.; Berdasco, M.; Urdinguio, R. G.; Fraga, M. F.; Jacinto, F. V.; Esteller, M.

    2009-01-01

    The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression levels increase during tumorigenesis. Here, we present evidence that the expression of DNMT3b is post-transcriptionally regulated by HuR, an RNA-binding protein that stabilizes and/or modulates the translation of target mRNAs. The presence of a putative HuR-recognition motif in the DNMT3b 3???UTR pr...

  12. Comparison of the Debye–Hückel and the Mean Spherical Approximation Theories for Electrolyte Solutions

    DEFF Research Database (Denmark)

    Maribo-Mogensen, Bjørn; Kontogeorgis, Georgios M.; Thomsen, Kaj

    2012-01-01

    The thermodynamics of electrolyte solutions has been investigated by many scientists throughout the last century. While several theories have been presented, the most popular models for the electrostatic interactions are based on the Debye–Hückel and mean spherical approximation (MSA) theories....... In this paper we investigate the differences between the Debye–Hückel and the MSA theories, and comparisons of the numerical results for the Helmholtz energy and its derivatives with respect to temperature, volume and composition are presented. The investigation shows that the nonrestricted primitive MSA...

  13. Anti-solar differential rotation on the active sub-giant HU Virginis

    Science.gov (United States)

    Harutyunyan, G.; Strassmeier, K. G.; Künstler, A.; Carroll, T. A.; Weber, M.

    2016-08-01

    Context. Measuring surface differential rotation (DR) on different types of stars is important when characterizing the underlying stellar dynamo. It has been suggested that anti-solar DR laws can occur when strong meridional flows exist. Aims: We aim to investigate the differential surface rotation on the primary star of the RS CVn binary, HU Vir, by tracking its starspot distribution as a function of time. We also aim to recompute and update the values for several system parameters of the triple system HU Vir (close and wide orbits). Methods: Time-series high-resolution spectroscopy for four continuous months was obtained with the 1.2-m robotic STELLA telescope. Nine consecutive Doppler images were reconstructed from these data, using our line-profile inversion code iMap. An image cross-correlation method was applied to derive the surface differential-rotation law for HU Vir. New orbital elements for the close and the wide orbits were computed using our new STELLA radial velocities (RVs) combined with the RV data available in the literature. Photometric observations were performed with the Amadeus Automatic Photoelectric Telescope (APT), providing contemporaneous Johnson-Cousins V and I data for approximately 20 yrs. This data was used to determine the stellar rotation period and the active longitudes. Results: We confirm anti-solar DR with a surface shear parameter α of -0.029 ± 0.005 and -0.026 ± 0.009, using single-term and double-term differential rotation laws, respectively. These values are in good agreement with previously claimed results. The best fit is achieved assuming a solar-like double-term law with a lap time of ≈400 d. Our orbital solutions result in a period of 10.387678 ± 0.000003 days for the close orbit and 2726 ± 7 d (≈7.5 yr) for the wide orbit. A Lomb-Scarge (L-S) periodogram of the pre-whitened V-band data reveals a strong single peak providing a rotation period of 10.391 ± 0.008 d, well synchronized to the short orbit. Based on

  14. 朱熹与张栻的论学:性体情用心统与性体心用导向心之九义%On the Argument between ZHU Xi and ZHANG Shi

    Institute of Scientific and Technical Information of China (English)

    成中英

    2014-01-01

    我们讨论朱熹与张栻的论辩,固然需要对文本和历史作出深刻的了解,这是经典诠释的基本要素,但是单纯的经典诠释无法获得本体层面的根源性了解,因之我们尚需采取本体诠释学的方法,从根源上寻求体系的发展与成立。在这个意义上,朱子和胡五峰、张南轩的湖湘学派,是相互引申与相互攻错的关系,而不应将之看作谁是谁非的关系。更重要的是,在这种相互引申、相互攻错的关系之中,吾人可以就孟子以来的心性之学提出九项意义,亦即“心之九义”,显示“心”的意义多形多样。我为了彰显与体会其心与性情之际的多形多样而一体之义,乃回顾了四书、二程、胡五峰、张南轩、朱子的学说,在此提出“心”之九种含义之说。%The discussion of the argument between ZHU Xi and ZHANG Shi needs to be based on the basic element of interpretation of classics, namely, the keen insight of literature and history. But the simple interpretation of classics cannot lead to the thorough understanding from ontology level. Ontological hermeneutics should be applied instead so as to look into the establishment and development of the sys-tem. In this sense, the thoughts of ZHU Xi and those of Huxiang school, with HU Wu-feng and ZHANG Nan-xuan as its representatives, are actually overlapping and complementary so that neither can be de-fined as definitely right or wrong. More importantly, based on their complementary relationship, nine points of Mencius’ “Mind has nine meanings” can be concluded, manifesting various meanings of mind. With reviews of the Four books, theories of CHENGs, theories of HU Wu-feng, theories of ZHANG Nan-xuan, and theories of ZHU Xi, the author proposes the theory of “mind has nine meanings”.

  15. Isolation and characterization of a catalase gene "HuCAT3" from pitaya (Hylocereus undatus) and its expression under abiotic stress.

    Science.gov (United States)

    Nie, Qiong; Gao, Guo-Li; Fan, Qing-jie; Qiao, Guang; Wen, Xiao-Peng; Liu, Tao; Peng, Zhi-Jun; Cai, Yong-Qiang

    2015-05-25

    Abiotic stresses usually cause H2O2 accumulation, with harmful effects, in plants. Catalase may play a key protective role in plant cells by detoxifying this excess H2O2. Pitaya (Hylocereus undatus) shows broad ecological adaptation due to its high tolerance to abiotic stresses, e.g. drought, heat and poor soil. However, involvement of the pitaya catalase gene (HuCAT) in tolerance to abiotic stresses is unknown. In the present study, a full-length HuCAT3 cDNA (1870 bp) was isolated from pitaya based on our previous microarray data and RACE method. The cDNA sequence and deduced amino acid sequence shared 73-77% and 75-80% identity with other plant catalases, respectively. HuCAT3 contains conserved catalase family domain and catalytic sites. Pairwise comparison and phylogenetic analysis indicated that HuCAT3 is most similar to Eriobotrya japonica CAT, followed by Dimocarpus longan CAT and Nicotiana tabacum CAT1. Expression profile analysis demonstrated that HuCAT3 is mainly expressed in green cotyledons and mature stems, and was regulated by H2O2, drought, cold and salt stress, whereas, its expression patterns and maximum expression levels varied with stress types. HuCAT activity increased as exposure to the tested stresses, and the fluctuation of HuCAT activity was consistent with HuCAT3 mRNA abundance (except for 0.5 days upon drought stress). HuCAT3 mRNA elevations and HuCAT activities changes under cold stress were also in conformity with the cold tolerances among the four genotypes. The obtained results confirmed a major role of HuCAT3 in abiotic stress response of pitaya. This may prove useful in understanding pitaya's high tolerance to abiotic stresses at molecular level.

  16. 葛根总黄酮体外诱导急性单核细胞白血病SHI-1细胞分化的实验研究%Experimental study on the differentiation of SHI-1 cells induced by puerariae radix flavones in vitro

    Institute of Scientific and Technical Information of China (English)

    朱国华; 张琦; 戴海萍; 翟云良; 沈群

    2013-01-01

    Objective To explore the possible effects on differentiation of SHI-1 cells induced by puerariae radix flavones(PRF)in vitro.Methods SHI-1 cells were treated with PRF in various concertration,then the inhibitory effects of cell proliferation were detected by MTT assay,the cell cycles were analyzed by flow cytometry(FCM),the cells reduction rates were detected by NBT reduction test,and the expression of CD11b and CD14 were tested by FCM.Results 10-50 μg/ml PRF could inhibit the proliferation of SHI-1 cells in a time-and dose-dependent manner,and the cell cycles were arrested in S phase.When SHI-1 cells were treated with 10,30 and 50 μg/ml PRF in 48 houres respectively,the NBT reduction rates of cells were increased in a dose-dependent with PRF(P<0.05),and the expression of cells surface differentiation antigen CD14 was also increased along with the concentration of PRF.Conclusion The SHI-1 cells could be induced to differentiation partially after treated with 10,30 and 50 μg/ml PRF in vitro.%目的 探讨葛根总黄酮(PRF)体外诱导人类急性单核细胞白血病SHI-1细胞发生部分分化的可能作用.方法 以不同浓度PRF作用SHI-1细胞不同时间,四甲基偶氮唑蓝比色法检测细胞增殖抑制率,流式细胞术(FCM)检测细胞周期,硝基四唑氮蓝(NBT)实验检测细胞还原率,FCM检测细胞表面分化抗原CD11b及CD14的表达.结果 10 ~ 50 μg/ml PRF呈时间-剂量依赖性抑制SHI-1细胞增殖,使细胞阻滞于S期.以10、30、50 μg/ml的PRF药物分别处理SHI-1细胞48 h,SHI-1细胞的NBT还原率随着药物浓度的升高而逐渐增高(P<0.05),SHI-1细胞表面的分化抗原CD14也同样随着药物浓度的升高而表达增高.结论 10、30、50μg/ml的PRF可体外诱导SHI-1细胞发生部分分化.

  17. Energy restriction affect liver development in Hu sheep ram lambs through Hippo signaling pathway.

    Science.gov (United States)

    Zhang, Ting-Ting; Zhang, Guo-Min; Jin, Yu-Hang; Guo, Yi-Xuan; Wang, Zhen; Fan, Yi-Xuan; El-Samahy, M A; Wang, Feng

    2017-09-02

    This study aimed to evaluate the effects of dietary energy restriction on postnatal liver development in Hu sheep ram lambs. A total of 16 ram lambs were randomly divided into two groups: 100% energy requirements diet and 55% energy requirements diet, which were fed for 75 d. Results showed that the final body and liver weights decreased with energy restriction (p Energy restriction caused a significant decrease in the levels of circulating insulin-like growth factor 1 (IGF1) and an increase in growth hormone secretion (p energy restriction, respectively (p energy restriction group (p energy restriction group (p energy restriction in ram lambs impairs liver development by increasing apoptosis, which may occur via the Hippo signaling pathway. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Flight Testing Surfaces Engineered for Mitigating Insect Adhesion on a Falcon HU-25C

    Science.gov (United States)

    Shanahan, Michelle; Wohl, Chris J.; Smith, Joseph G., Jr.; Connell, John W.; Siochi, Emilie J.; Doss, Jereme R.; Penner, Ronald K.

    2015-01-01

    Insect residue contamination on aircraft wings can decrease fuel efficiency in aircraft designed for natural laminar flow. Insect residues can cause a premature transition to turbulent flow, increasing fuel burn and making the aircraft less environmentally friendly. Surfaces, designed to minimize insect residue adhesion, were evaluated through flight testing on a Falcon HU-25C aircraft flown along the coast of Virginia and North Carolina. The surfaces were affixed to the wing leading edge and the aircraft remained at altitudes lower than 1000 feet throughout the flight to assure high insect density. The number of strikes on the engineered surfaces was compared to, and found to be lower than, untreated aluminum control surfaces flown concurrently. Optical profilometry was used to determine insect residue height and areal coverage. Differences in results between flight and laboratory tests suggest the importance of testing in realistic use environments to evaluate the effectiveness of engineered surface designs.

  19. The Curious Case of HU Aquarii - Dynamically Testing Proposed Planetary Systems

    CERN Document Server

    Horner, Jonathan; Marshall, Jonathan P; Tinney, Chris G; Butters, Oliver W

    2012-01-01

    In early 2011, the discovery of two planets moving on surprisingly extreme orbits around the eclipsing polar cataclysmic variable system HU Aquraii was announced based on variations in the timing of mutual eclipses between the two central stars. We perform a detailed dynamical analysis of the stability of the exoplanet system as proposed in that work, revealing that it is simply dynamically unfeasible. We then apply the latest rigorous methods used by the Anglo-Australian Planet Search to analyse radial velocity data to re-examine the data used to make the initial claim. Using that data, we arrive at a significantly different orbital solution for the proposed planets, which we then show through dynamical analysis to be equally unfeasible. Finally, we discuss the need for caution in linking eclipse-timing data for cataclysmic variables to the presence of planets, and suggest a more likely explanation for the observed signal.

  20. Congenital Pelger-Huët anomaly in a Danish/Swedish Farmdog: Case Report

    Directory of Open Access Journals (Sweden)

    Allison Robin W

    2011-03-01

    Full Text Available Abstract A 13 year old Danish/Swedish Farmdog from Denmark was evaluated in a veterinary clinic in Warsaw, Poland for evaluation of an orthopedic problem. Radiographs revealed spondylosis and degenerative vertebral disease, which responded to treatment with anti-inflammatory medications. A predominance of hyposegmented neutrophils and eosinophils containing condensed chromatin and normal cytoplasm were identified on a routine CBC. Follow-up blood film evaluations over the course of 12 months confirmed that the hyposegmented granulocytes persisted. The majority of neutrophils contained Grade 2 nuclei (slightly indented, and the mean nuclear score varied from 1.9 to 2.3. Pelger-Huët anomaly (PHA, presumably congenital, was diagnosed based on persistent hyposegmented granulocytes in the absence of an underlying cause for acquired PHA; genetically related dogs were unavailable for testing to confirm vertical transmission. To the authors' knowledge this is the first report of PHA in a Danish/Swedish Farmdog.

  1. Potential therapeutic use of antibodies directed towards HuIFN-gamma.

    Science.gov (United States)

    Froyen, G; Billiau, A

    1997-01-01

    IFN-gamma is an important regulator of immune responses and inflammation. Studies in animal models of inflammation, autoimmunity, cancer, transplant rejection and delayed-type hypersensitivity have indicated that administration of antibodies against IFN-gamma can prevent the occurrence of diseases or alleviate disease manifestations. Therefore, it is speculated that such antibodies may have therapeutical efficacy in human diseases. Since animal-derived antibodies are immunogenic in patients several strategies are being developed in order to reduce or abolish this human anti-mouse antibody (HAMA) response. In our laboratory, we have constructed a single-chain variable fragment (scFv) derived from a mouse antibody with neutralizing potential for human IFN-gamma. A scFv consists of only variable domains tethered together by a flexible linker. The scFv was demonstrated to neutralize the antiviral activity of HuIFN-gamma in vitro and therefore might be considered as a candidate for human therapy.

  2. La voz y la letra en "Un huésped", de Yoko Tawada

    Directory of Open Access Journals (Sweden)

    María Lucía Correa

    2009-05-01

    Full Text Available Los textos de Yoko Tawada, escritorajaponesa residente en Alemania,hacen presente el desgarramientode quien vive fuera de la lenguamaterna. Es una obra que se balanceasobre el límite entre loscódigos, donde nada hay evidente,donde la palabra parece tener vidapropia. Entre los textos que tomanesta extrañeza del lenguaje comosustrato, se encuentra el relato"Un huésped", cuya particular referenciaa la voz y a la letra comodos registros que se entrelazan yse repelen alternativamente, loconvierte en una valiosa fuente dereflexiones sobre lo oral, la voz, laletra, la lectura y la escritura.Palabras clave: Yoko Tawada, literaturajaponesa contemporánea, voz,letra, escritura.

  3. The SCID-hu mouse as a tool in immunotoxicological risk assessment: Effects of 2-acetyl-4(5)-tetrahydroxybutyl-imidazole (THI) and di-n-butyltin dichloride (DBTC) on the human thymus in SCID-hu mice

    NARCIS (Netherlands)

    Heer, C. de; Schuurman, H.J.; Houben, G.F.; Pieters, R.H.H.; Penninks, A.H.; Loveren, H. van

    1995-01-01

    SCID mice engrafted with human fetal thymus and liver tissue fragments (SCID-hu mice) are currently considered as a new tool in human immunotoxicological risk assessment. Testing of various immunotoxicants exerting thymotoxicity via different intrathymic target cell types is necessary for validation

  4. The complete chloroplast genome sequence of the relict woody plant Metasequoia glyptostroboides Hu et Cheng.

    Science.gov (United States)

    Chen, Jinhui; Hao, Zhaodong; Xu, Haibin; Yang, Liming; Liu, Guangxin; Sheng, Yu; Zheng, Chen; Zheng, Weiwei; Cheng, Tielong; Shi, Jisen

    2015-01-01

    Metasequoia glyptostroboides Hu et Cheng is the only species in the genus Metasequoia Miki ex Hu et Cheng, which belongs to the Cupressaceae family. There were around 10 species in the Metasequoia genus, which were widely spread across the Northern Hemisphere during the Cretaceous of the Mesozoic and in the Cenozoic. M. glyptostroboides is the only remaining representative of this genus. Here, we report the complete chloroplast (cp) genome sequence and the cp genomic features of M. glyptostroboides. The M. glyptostroboides cp genome is 131,887 bp in length, with a total of 117 genes comprised of 82 protein-coding genes, 31 tRNA genes and four rRNA genes. In this genome, 11 forward repeats, nine palindromic repeats, and 15 tandem repeats were detected. A total of 188 perfect microsatellites were detected through simple sequence repeat (SSR) analysis and these were distributed unevenly within the cp genome. Comparison of the cp genome structure and gene order to those of several other land plants indicated that a copy of the inverted repeat (IR) region, which was found to be IR region A (IRA), was lost in the M. glyptostroboides cp genome. The five most divergent and five most conserved genes were determined and further phylogenetic analysis was performed among plant species, especially for related species in conifers. Finally, phylogenetic analysis demonstrated that M. glyptostroboides is a sister species to Cryptomeria japonica (L. F.) D. Don and to Taiwania cryptomerioides Hayata. The complete cp genome sequence information of M. glyptostroboides will be great helpful for further investigations of this endemic relict woody plant and for in-depth understanding of the evolutionary history of the coniferous cp genomes, especially for the position of M. glyptostroboides in plant systematics and evolution.

  5. The Analysis of Shi Jianwu's Birth-and-death Dates%(唐)施肩吾生卒年限推断

    Institute of Scientific and Technical Information of China (English)

    詹飘飘

    2012-01-01

    本文从施肩吾的诗文作品着手,找寻能够显示施肩吾年龄或生存年代的线索,从施肩吾曾下第的经历,以及诗句"年来如抛梭,不老应不得"中折射出的沧桑感,判断出施肩吾进士及第时已人到中年,由此推出他可能出生于唐德宗建中-贞元年间。又据施肩吾的《述灵响词序》和《西山集》序言提供的两个时间线索,推出唐文宗开成六年施肩吾仍然在世。而且,施肩吾的《与徐凝书》和《喜友再相逢》,也证实了他的迟暮。最后结合《吴兴备志》和《施氏宗谱》的说法,综合考量,暂定施肩吾的生卒年限为唐德宗建中元年(780)——唐懿宗咸通二年(861)之间。%The thesis tries to find out some clues about Shi Jianwu's birth-and-death dates from his literature.Firstly,according to the experience that Shi Jianwu once failed in the examination getting the qualification to be an official,as well as his poem "Time flies,only if be old can get the qualification" which reflected a sense of vicissitudes,it could tell that Shi Jianwu was an old man when he passed the examination.So he may be born between "Jian-Zhong" to "Zhen-Yuan" under the rule of emperor "DeZhong" on Tang Dynasty.Then,another two articles written by Shi Jianwu contained two time clues,which told Shi Jianwu had still been alive in the sixth year of "Kai-Cheng" under the rule of emperor "Wen Zhong" on Tang Dynasty.And it could be proved that Shi Jianwu's longevity through a letter to Ning Xu and happy to be met again with friend written by Shi Jianwu too.Above all,combining the records from Wu-Xing records and genealogy of Shi as the last name,Jianwu Shi' birth-and-death dates could be limited between the first year of "Jian-Zhong" ruled by emperor "De Zhong" to the second year of "Xian-Tong" ruled by "Yi Zhong" on Tang Dynasty,which means that Jianwu Shi may be born in AD 780 and dead in AD 861.

  6. Antidepressant effects of curcumin and HU-211 coencapsulated solid lipid nanoparticles against corticosterone-induced cellular and animal models of major depression

    Science.gov (United States)

    He, Xiaolie; Zhu, Yanjing; Wang, Mei; Jing, Guoxin; Zhu, Rongrong; Wang, Shilong

    2016-01-01

    Major depression is a complex neuropsychiatric disorder with few treatment approaches. The use of nontargeted antidepressants induced many side effects with their low efficacy. A more precise targeting strategy is to develop nanotechnology-based drug delivery systems; hence, we employed solid lipid nanoparticles (SLNs) to encapsulate HU-211 and curcumin (Cur). The antidepressant effects of the dual-drug nanoparticles (Cur/SLNs-HU-211) for major depression treatment were investigated in corticosterone-induced cellular and animal models of major depression. Cur/SLNs-HU-211 can effectively protect PC12 cells from corticosterone-induced apoptosis and can release more dopamine, which may be associated with the higher uptake of Cur/SLNs-HU-211 shown by cellular uptake behavior analysis. Additionally, Cur/SLNs-HU-211 significantly reduced the immobility time in forced swim test, enhanced fall latency in rotarod test, and improved the level of dopamine in mice blood. Cur/SLNs-HU-211 can deliver more Cur to the brain and thus produce a significant increase in neurotransmitters level in brain tissue, especially in the hippocampus and striatum. The results of Western blot and immunofluorescence revealed that Cur/SLNs-HU-211 can significantly enhance the expression of CB1, p-MEK1, and p-ERK1/2. Our study suggests that Cur/SLNs-HU-211 may have great potential for major depression treatment. PMID:27757031

  7. HuD interacts with Bdnf mRNA and is essential for activity-induced BDNF synthesis in dendrites.

    Directory of Open Access Journals (Sweden)

    Filip Vanevski

    Full Text Available Highly specific activity-dependent neuronal responses are necessary for modulating synapses to facilitate learning and memory. We present evidence linking a number of important processes involved in regulating synaptic plasticity, suggesting a mechanistic pathway whereby activity-dependent signaling, likely through protein kinase C (PKC-mediated phosphorylation of HuD, can relieve basal repression of Bdnf mRNA translation in dendrites, allowing for increased TrkB signaling and synaptic remodeling. We demonstrate that the neuronal ELAV family of RNA binding proteins associates in vivo with several Bdnf mRNA isoforms present in the adult brain in an activity-dependent manner, and that one member, HuD, interacts directly with sequences in the long Bdnf 3' untranslated region (3'UTR and co-localizes with Bdnf mRNA in dendrites of hippocampal neurons. Activation of PKC leads to increased dendritic translation of mRNAs containing the long Bdnf 3'UTR, a process that is dependent on the presence of HuD and its phosphorylation at threonine residues 149 and/or 165. Thus, we found a direct effect of HuD on regulating translation of dendritic Bdnf mRNAs to mediate local and activity-dependent increases in dendritic BDNF synthesis.

  8. Genetic diversity and population structure in the narrow endemic Chinese walnut Juglans hopeiensis Hu: implications for conservation

    Science.gov (United States)

    Yiheng Hu; Meng Dang; Xiaojia Feng; Keith Woeste; Peng Zhao

    2017-01-01

    The conservation of narrow endemic species relies on accurate information regarding their population structure. Juglans hopeiensis Hu (Ma walnut), found only in Hebei province, Beijing, and Tianjin, China, is a threatened tree species valued commercially for its nut and wood. Sequences of two maternally inherited mitochondrial markers and two...

  9. The Preliminary Research on Rupture and Factors Affecting Rupturing of the Hoh Sai Hu Segment in Eastern Kunlun

    Institute of Scientific and Technical Information of China (English)

    Bai Yuzhu; Xu Jie; Zhou Qing; Zhou Bengang

    2012-01-01

    On November 14, 2001, an extraordinarily large earthquake (Ms8. 1) occurred on the Hoh Sai Hu segment of the Eastern Kunlun Fault, in the northern Qinghai-Tibetan Plateau. The seismogenic fault, the Hoh Sai Hu segment, is a left-lateral fault with a high slip rate in geological history, with the average slip rate reaching( 14. 8± 2. 8)mm/a since the late Pleistocene. Different slip rates of the Hoh Sai Hu segment can affect fault motion in the future. Therefore, this paper analyzes the effect of different slip rates and different initial friction coefficients on the fault plane of the Hoh Sai Hu segment of the eastern Kunlun Fault on the rupture behaviors of the fault. In this research, we apply the single degree of spring block model controlled by the rate- and state-dependent frictional constitutive laws. Using the fault dislocation model and based on ancient earthquake research, historical earthquakes data and the achievements of previous researchers, we obtained the parameters of this model. Through numerical simulation of the rupturing motion of the Hoh Sai Hu segment in the next 6500 years under different slip rates, we find that a faster annual slip rate will shorten the recurrence interval of the earthquake. For example, the earthquake recurrence interval is 2100a at a slip rate of 0. 014m/a, which agrees with previous research, but, the recurrence interval will be 1000 - 1500a and 2100 - 2500a, corresponding to the slip rates of 0. 018m/a and 0. 008m/a, respectively. The fault slip rate has no regular effect on the coseismic slip rate and fault displacement in an earthquake. The initial friction coefficient on the fault surface has an effect on the earthquake recurrence interval. A smaller initial friction coefficient will lengthen the earthquake recurrence interval. At the same time, the smaller initial friction coefficient will lead to larger slip rates and fault displacement when earthquakes occur.

  10. Striatal synaptic dysfunction and hippocampal plasticity deficits in the Hu97/18 mouse model of Huntington disease.

    Directory of Open Access Journals (Sweden)

    Karolina Kolodziejczyk

    Full Text Available Huntington disease (HD is a fatal neurodegenerative disorder caused by a CAG repeat expansion in the gene (HTT encoding the huntingtin protein (HTT. This mutation leads to multiple cellular and synaptic alterations that are mimicked in many current HD animal models. However, the most commonly used, well-characterized HD models do not accurately reproduce the genetics of human disease. Recently, a new 'humanized' mouse model, termed Hu97/18, has been developed that genetically recapitulates human HD, including two human HTT alleles, no mouse Hdh alleles and heterozygosity of the HD mutation. Previously, behavioral and neuropathological testing in Hu97/18 mice revealed many features of HD, yet no electrophysiological measures were employed to investigate possible synaptic alterations. Here, we describe electrophysiological changes in the striatum and hippocampus of the Hu97/18 mice. At 9 months of age, a stage when cognitive deficits are fully developed and motor dysfunction is also evident, Hu97/18 striatal spiny projection neurons (SPNs exhibited small changes in membrane properties and lower amplitude and frequency of spontaneous excitatory postsynaptic currents (sEPSCs; however, release probability from presynaptic terminals was unaltered. Strikingly, these mice also exhibited a profound deficiency in long-term potentiation (LTP at CA3-to-CA1 synapses. In contrast, at 6 months of age we found only subtle alterations in SPN synaptic transmission, while 3-month old animals did not display any electrophysiologically detectable changes in the striatum and CA1 LTP was intact. Together, these data reveal robust, progressive deficits in synaptic function and plasticity in Hu97/18 mice, consistent with previously reported behavioral abnormalities, and suggest an optimal age (9 months for future electrophysiological assessment in preclinical studies of HD.

  11. Alternativa terapeutica en la isquemia cerebral empleando la Eritropoyetina humana recombinante (rHu-EPO CIM como neuroprotector.

    Directory of Open Access Journals (Sweden)

    Iliana Sosa Testé

    2005-01-01

    Full Text Available Las enfermedades vasculares constituyen las terceras causas de muerte en Cuba. Su variante isquémica es causa de invalidez y perdida de la calidad de la vida. La búsqueda de agentes neuroprotectores es muy importante sí tenemos en cuenta que nuestra población está envejecida. Muchos son las razones por la que una molécula con propiedades neuroprotectoras en los modelos animales no tenga los resultados en ensayos clínicos en humanos. Este trabajo analiza y discute como mejorar estos aspectos. La Eritropoyetina humana recombinante (rHu EPO, conocido por su papel en la diferenciación del eritrocito, ha mostrado ser neuroprotector durante la isquemia cerebral en los modelos de animales adultos. De esta molécula se exponen los mecanismos de acción, que son las bases de los resultados positivos alcanzaron en las investigaciones preclínicas y los ensayos clínicos, que lo proponen como el neuroprotector ideal. Finalmente se comparan los resultados alcanzados por los autores en las investigaciones con la rHu EPO producida por el Centro Molecular de Inmunología de Cuba (CIM. El efecto de la aplicación de rHu EPO se evidenció por la disminución del edema cerebral, preservación de la conducta exploratoria y la disminución de la incidencia de señales clínicas de infarto cerebral y alteraciones patológicas. El grupo de resultados demostró las diferencias significantes entre los animales controles y tratados con el rHu EPO con los no tratados que sugieren que el rHu EPO debe ser considerado como una alternativa terapéutica contra el infarto cerebral en su fase aguda.

  12. A novel high throughput biochemical assay to evaluate the HuR protein-RNA complex formation.

    Directory of Open Access Journals (Sweden)

    Vito G D'Agostino

    Full Text Available The RNA binding protein HuR/ELAVL1 binds to AU-rich elements (AREs promoting the stabilization and translation of a number of mRNAs into the cytoplasm, dictating their fate. We applied the AlphaScreen technology using purified human HuR protein, expressed in a mammalian cell-based system, to characterize in vitro its binding performance towards a ssRNA probe whose sequence corresponds to the are present in TNFα 3' untranslated region. We optimized the method to titrate ligands and analyzed the kinetic in saturation binding and time course experiments, including competition assays. The method revealed to be a successful tool for determination of HuR binding kinetic parameters in the nanomolar range, with calculated Kd of 2.5±0.60 nM, k on of 2.76±0.56*10(6 M(-1 min(-1, and k off of 0.007±0.005 min(-1. We also tested the HuR-RNA complex formation by fluorescent probe-based RNA-EMSA. Moreover, in a 384-well plate format we obtained a Z-factor of 0.84 and an averaged coefficient of variation between controls of 8%, indicating that this biochemical assay fulfills criteria of robustness for a targeted screening approach. After a screening with 2000 small molecules and secondary verification with RNA-EMSA we identified mitoxantrone as an interfering compound with rHuR and TNFα probe complex formation. Notably, this tool has a large versatility and could be applied to other RNA Binding Proteins recognizing different RNA, DNA, or protein species. In addition, it opens new perspectives in the identification of small-molecule modulators of RNA binding proteins activity.

  13. A study on the Statement of Duality of the Origin of Chinese Characters from Xu and Shi of Things and Symbols%从事物和符号的虚实论汉字起源二元说

    Institute of Scientific and Technical Information of China (English)

    蒋瑞

    2016-01-01

    As things have xu and shi situations,the symbols to reflect things also have xu and shi. The dichotomy of early Chinese characters between pictographs and ideographs which signify xu and shi respectively proves that Chinese characters originated from duality.The dichotomous practice of xu and shi of prehistoric pictographic and ideographic symbols have prepared for the origin of Chinese characters in methods.%由于事物有虚实,则反映事物的符号也有虚实。早期汉字象形和指事一实一虚的二分,证明了汉字起源于二元。史前象形性符号和指事性符号一实一虚的二分实践,则从方法上为汉字起源于二元做好了铺垫。

  14. A new phantom for image quality, geometric destortion, and HU calibration in MSCT and CBCT

    Science.gov (United States)

    Voigt, Johannes M.; Blendl, Christian; Selbach, Markus; Uphoff, Clemens; Fiebich, Martin

    2012-03-01

    Flat panel cone-beam computed tomography (CBCT) is developing to the state-of-the-art technique in several medical disciplines such as dental and otorhinolaryngological imaging. Dental and otorhinolaryngological CBCT systems offer a variety of different field-of-view sizes from 6.0 to 17.0 cm. Standard phantoms are only designed for the use in multi-slices CT (MSCT) and there is no phantom which provides detail structures for all common characteristic values and Hounsfield calibration. In this study we present a new phantom specially designed for use with MSCT and CBCT systems providing detail structures for MTF, 3D MTF, NPS, SNR, geometric distortion and HU calibration. With this phantom you'll only need one acquisition for image quality investigation and assurance. Materials and methods: The phantom design is shown in figure 1. To investigate the practicability, the phantom was scanned using dedicated MSCT-scanners, 3D C-arms und digital volume tomographs. The acquired axial image stacks were analyzed using a dedicated computer program, which is provided as an ImageJ plugin. The MTF was compared to other methodologies such as a thin wire, a sphere or noise response [10, 13, 14]. The HU values were also computed using other common methods. Results: These results are similar to the results of others studies [10, 13, 14]. The method has proven to be stable and delivers comparable results to other methodologies such as using a thin wire. The NPS was calculated for all materials. Furthermore, CT numbers for all materials were computed and compared to the desired values. The measurement of geometric deformation has proven to be accurate. Conclusion: A unique feature of this phantom is to compute the geometric deformation of the 3D-volume image. This offers the chance to improve accuracy, e.g. in dental implant planning. Another convenient feature is that the phantom needs to be scanned only once with otorhinolaryngological volume tomographs to be fully displayed. It is

  15. {sup 89}Zr-huJ591 immuno-PET imaging in patients with advanced metastatic prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Pandit-Taskar, Neeta; Solomon, Stephen B.; Durack, Jeremy C.; Carrasquillo, Jorge A.; Lefkowitz, Robert A.; Osborne, Joseph R. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); O' Donoghue, Joseph A. [Memorial Sloan Kettering Cancer Center, Department of Medical Physics, New York, NY (United States); Beylergil, Volkan; Ruan, Shutian; Cheal, Sarah M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Lyashchenko, Serge [Memorial Sloan Kettering Cancer Center, Department of Radiochemistry and Molecular Imaging Probes Core, New York, NY (United States); Gonen, Mithat [Memorial Sloan Kettering Cancer Center, Department of Epidemiology and Biostatistics, New York, NY (United States); Lewis, Jason S. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Department of Radiochemistry and Molecular Imaging Probes Core, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Program in Molecular Pharmacology and Chemistry, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); Holland, Jason P. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Harvard Medical School, Department of Radiology of Massachusetts General Hospital, Boston, MA (United States); Reuter, Victor E. [Memorial Sloan Kettering Cancer Center, Department of Pathology, New York, NY (United States); Weill Cornell Medical College, Department of Pathology, New York, NY (United States); Loda, Massimo F. [Dana-Farber Cancer Institute, Boston, MA (United States); Broad Institute of Harvard and MIT, Cambridge, MA (United States); Smith-Jones, Peter M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Department of Psychiatry and Behavioral Science of Stony Brook University, Stony Brook, NY (United States); Weber, Wolfgang A.; Larson, Steven M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Program in Molecular Pharmacology and Chemistry, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); Bander, Neil H. [Memorial Sloan Kettering Cancer Center, Department of Surgery, New York, NY (United States); Weill Cornell Medical College, Department of Urology, New York, NY (United States); Scher, Howard I.; Morris, Michael J. [Memorial Sloan Kettering Cancer Center, Department of Medicine, New York, NY (United States); Weill Cornell Medical College, Department of Medicine, New York, NY (United States)

    2014-11-15

    Given the bone tropism of prostate cancer, conventional imaging modalities poorly identify or quantify metastatic disease. {sup 89}Zr-huJ591 positron emission tomography (PET) imaging was performed in patients with metastatic prostate cancer to analyze and validate this as an imaging biomarker for metastatic disease. The purpose of this initial study was to assess safety, biodistribution, normal organ dosimetry, and optimal imaging time post-injection for lesion detection. Ten patients with metastatic prostate cancer received 5 mCi of {sup 89}Zr-huJ591. Four whole-body scans with multiple whole-body count rate measurements and serum activity concentration measurements were obtained in all patients. Biodistribution, clearance, and lesion uptake by {sup 89}Zr-huJ591 immuno-PET imaging was analyzed and dosimetry was estimated using MIRD techniques. Initial assessment of lesion targeting of {sup 89}Zr-huJ591 was done. Optimal time for imaging post-injection was determined. The dose was well tolerated with mild chills and rigors seen in two patients. The clearance of {sup 89}Zr-huJ591 from serum was bi-exponential with biological half-lives of 7 ± 4.5 h (range 1.1-14 h) and 62 ± 13 h (range 51-89 h) for initial rapid and later slow phase. Whole-body biological clearance was 219 ± 48 h (range 153-317 h). The mean whole-body and liver residence time was 78.7 and 25.6 h, respectively. Dosimetric estimates to critical organs included liver 7.7 ± 1.5 cGy/mCi, renal cortex 3.5 ± 0.4 cGy/mCi, and bone marrow 1.2 ± 0.2 cGy/mCi. Optimal time for patient imaging after injection was 7 ± 1 days. Lesion targeting of bone or soft tissue was seen in all patients. Biopsies were performed in 8 patients for a total 12 lesions, all of which were histologically confirmed as metastatic prostate cancer. One biopsy-proven lesion was not positive on {sup 89}Zr-huJ591, while the remaining 11 lesions were {sup 89}Zr-huJ591 positive. Two biopsy-positive nodal lesions were noted only on

  16. Discussion the Theory of "Shi's Twelve Word Life Cultivation Skill"%“施氏十二字养生功”的基础理论探讨

    Institute of Scientific and Technical Information of China (English)

    胡志俊; 王世伟; 施杞; 叶秀兰; 唐占英

    2011-01-01

    目的:探讨施氏十二字养生功理论渊源及作用原理.方法:通过发掘古代医学文献对导引的论述并结合现代医学理论来探讨施氏十二字养生功的作用机理.结果:施氏十二字养生功是在中医传统导引理论指导下通过长期的临床实践创建的养生功,对以颈椎病为主的骨退行性病变引起的疼痛不适等有良好疗效.结论:施氏十二字养生功是防治颈椎病有效方法之一.%Objective: Discussion the origin of "Shi's twelve word life cultivation skill " theory and principle. Methods: To explore the ancient medical literature through the discussion on the guidance and combined with modern medical theory of health work Discussion the theory of "Shi's twelve word life cultivation skill" and it's mechanism. Results: "Shi's twelve word life cultivation skill" is guided in the theory of traditional Chinese medicine under the guidance of clinical practice through long-term work to create the health of the cervical degenerative bone disease mainly caused such pain and discomfort have a good effect. Conclusion: "Shi's twelve-word life-cultivation skill" is an effective method for prevention and treatment of cervical spondylosis.

  17. 白驹施耐庵的历史真实三证%The Three Evidences of Shi Naian' s Hometown Baiju Township

    Institute of Scientific and Technical Information of China (English)

    浦玉生

    2011-01-01

    The paper values oral transmission messages, combines written records, unearthed relics and oral literature, makes comprehensive, systematic and holographic considerations, cross checks and finally draws the conclusion that Shi Naian' s hometown is Baiju Township, which is the historic fact and goes a step further in inter- preting the mysteries of Shi Naian. Meanwhile, the paper is critical of the false claims that Xinghua of Yancheng is Shi Naian' s hometown, and Dafeng built Shi Naian' s cemetery. It points out that the people who reject Baiju Township make some errors out of lack of thorough investigation and one - sided ways.%本文重视口传史料,从文献记载、出土文物、民间口碑等三方面综合、系统、全息地思考,互相印证,从而证明白驹施耐庵的历史真实,在破译施耐庵之谜的道路上又向前推进了一步。同时,本文对当前施耐庵研究领域出现的所谓“盐城兴化”施耐庵(彦端)、大丰修建施耐庵陵园的不实之词进行了批评,指出否认白驹施耐庵的人未能深入调查:方法片面,以致闹出不应有的谬误。

  18. Mechanism of RNA binding protein HuR in regulating the activity of prostate cancer cells%RNA结合蛋白HuR调控前列腺癌细胞活力机制的研究

    Institute of Scientific and Technical Information of China (English)

    周宁; 程娟; 钟毅; 冉新建; 于文燕; 马琪

    2016-01-01

    目的 前列腺癌在我国的发病率及病死率逐年上升,但其发病机制尚未明确,本研究从转录后水平初步探讨RNA结合蛋白HuR参与调控前列腺癌细胞活力的机制.方法 免疫荧光检测前列腺增生细胞(BPH)及前列腺癌细胞(PC3)中HuR蛋白的定位,蛋白质印迹法检测HuR及环氧合酶-2(cyclooxygenase,COX-2)蛋白在细胞内的表达,MTT法检测细胞活力,脂质体转染法转染质粒及干扰片段,qPCR检测干扰效率,RNA-pull down实验验证HuR对COX-2的调控.结果 HuR在BPH细胞主要定位于细胞核,在PC3细胞中主要定位于胞质.PC3细胞中HuR表达水平为1.699±0.011,高于BPH细胞的0.654±0.028,差异有统计学意义,t=58.67,P<0.001.PC3细胞中过表达HuR后,转染空质粒组细胞活力为1.038±0.117,转染HuR表达质粒后细胞活力为1.838±0.057,差异有统计学意义,t=10.656,P<0.001.干扰HuR表达后,转染siNC组细胞活力为1.254±0.095,转染siHuR干扰片段组细胞活力为0.66±0.102,t=7.383,P=0.002;BPH组和PC3组COX-2表达水平分别为0.449±0.055和1.066±0.068,t=12.147,P<0.001;过表达COX-2后PC3细胞活力明显增加,转染空质粒组细胞活力为1.296±0.114,转染COX-2表达质粒后细胞活力为1.954±0.062,t=18.062,P<0.001.干扰COX-2表达后,PC3细胞活力明显降低,转染siNC组细胞活力为1.233±0.145,转染siCOX-2干扰片段后细胞活力为0.661±0.096,t=5.688,P=0.005.HuR可以结合于COX-2的3′-UTR并上调COX-2蛋白表达,转染空质粒组COX-2蛋白表达水平为0.638±0.067,转染HuR表达质粒组为1.703±0.022,t=26.26,P<0.001.结论 HuR参与调控前列腺癌细胞活力可能是通过上调COX-2表达而实现.

  19. Detection of neutralizing antibodies to erythropoietin by inhibition of rHuEPO-stimulated EGR1 gene expression in the UT-7/EPO cell line.

    Science.gov (United States)

    Ferguson, Jackie; Bird, Chris; Wadhwa, Meenu; Burns, Chris

    2013-01-31

    Recombinant erythropoietin (rHuEPO) is used extensively to treat anaemia associated with chronic kidney disease. However, the development of neutralizing antibodies (NAbs) to rHuEPO can result in the development of antibody-mediated pure red cell aplasia (PRCA). The detection of NAb in patient sera by in vitro bioassay relies on the inhibition of a cellular response to rHuEPO. Current bioassays for rHuEPO measure proliferation in responsive cell lines such as the erythroleukaemic cell lines, UT-7 and UT-7/EPO, the latter sensitized to EPO. Using these cell lines, we show the dose-responsive induction of both PIM1 and EGR1 gene expression in UT-7 cells and of EGR1 in UT-7/EPO cells. The expression of EGR1 in UT-7/EPO cells in response to rHuEPO was comparable to the proliferative response measured by (3)H-thymidine incorporation and could be inhibited by serum from a patient with NAb-mediated PRCA in a dilution-dependent manner. Bioassays based on the induction of endogenous gene expression are comparable to current bioassays but are considerably quicker given that incubation time is decreased from 2-3 days to 50 min. Measurement of EGR1 gene expression in response to rHuEPO in UT-7/EPO cells offers a rapid, non-radioactive and automatable alternative to current assays for the detection of rHuEPO NAbs.

  20. Testing Hu-Sawicki f(R) gravity with the Effective Field Theory approach

    CERN Document Server

    Hu, Bin; Rizzato, Matteo; Silvestri, Alessandra

    2016-01-01

    We show how to fully map a specific model of modified gravity into the Einstein-Boltzmann solver EFTCAMB. This approach consists in few steps and allows to obtain the cosmological phenomenology of a model with minimal effort. We discuss all these steps, from the solution of the dynamical equations for the cosmological background of the model to the use of the mapping relations to cast the model into the effective field theory language and use the latter to solve for perturbations. We choose the Hu-Sawicki f(R) model of gravity as our working example. After solving the background and performing the mapping, we interface the algorithm with EFTCAMB and take advantage of the effective field theory framework to integrate the full dynamics of linear perturbations, returning all quantities needed to accurately compare the model with observations. We discuss some observational signatures of this model, focusing on the linear growth of cosmic structures. In particular we present the behavior of $f\\sigma_8$ and $E_G$ t...

  1. La huída en la obra de Mario Vargas Llosa

    Directory of Open Access Journals (Sweden)

    German Arce Ross

    Full Text Available Se trata de poner en perspectiva la mayor parte de novelas de Mario Vargas Llosa en función del tema de la huída, partiendo del hecho que é1 solicitó la nacionalidad Española inmediatamente después de haber perdido las elecciones para la Presidencia del Perú y de haber escrito El pez en el agua. Nuestro análisis intenta demostrar la importancia de la figura paterna – principalmente en el sentido de su ausencia y de sus bruscas reapariciones – en la cristalización sintomática del tema de la fuga. Es por eso que una analogía con la posición fóbica, en relación al padre o a sus substitutos, nos ha parecido pertinente. Teniendo como pivot el análisis de la forma, del contexto y del contenido de su libro de memorias, este trabajo definiría así las bases de una contribución literaria de la obra de Vargas Llosa a la clinica de la fobia.

  2. Systematics of a kleptoplastidal dinoflagellate, Gymnodinium eucyaneum Hu (Dinophyceae, and its cryptomonad endosymbiont.

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    Shuang Xia

    Full Text Available New specimens of the kleptoplastidal dinoflagellate Gymnodinium eucyaneum Hu were collected in China. We investigated the systematics of the dinoflagellate and the origin of its endosymbiont based on light morphology and phylogenetic analyses using multiple DNA sequences. Cells were dorsoventrally flattened with a sharply acute hypocone and a hemispherical epicone. The confusion between G. eucyaneum and G. acidotum Nygaard still needs to be resolved. We found that the hypocone was conspicuously larger than the epicone in most G. eucyaneum cells, which differed from G. acidotum, but there were a few cells whose hypocone and epicone were of nearly the same size. In addition, there was only one site difference in the partial nuclear LSU rDNA sequences of a sample from Japan given the name G. acidotum and G. eucyaneum in the present study, which suggest that G. eucyaneum may be a synonym of G. acidotum. Spectroscopic analyses and phylogenetic analyses based on nucleomorph SSU rDNA sequences and chloroplast 23 s rDNA sequences suggested that the endosymbiont of G. eucyaneum was derived from Chroomonas (Cryptophyta, and that it was most closely related to C. coerulea Skuja. Moreover, the newly reported kleptoplastidal dinoflagellates G. myriopyrenoides and G. eucyaneum in our study were very similar, and the taxonomy of kleptoplastidal dinoflagellates was discussed.

  3. Screening of a xylanase clone from a fosmid library of rumen microbiota in Hu sheep.

    Science.gov (United States)

    Wang, Jiakun; Sun, Zhongyuan; Zhou, Yan; Wang, Qian; Ye, Jun'an; Chen, Zhenming; Liu, Jianxin

    2012-01-01

    The glycosyl hydrolase family 11, which is responsible for carbohydrate metabolism, was identified in the open reading frame (ORF) 6 of a xylanase positive clone from a fosmid library of rumen microbiota of Hu sheep. A BLASTP search of GenBank revealed that ORF6 encoded a 355-amino acid putative endoxylanase, having 61% similarity (e(-73)) to endo-1,4-β-xylanase of Fibrobacter succinogenes S85 (YP_003250510.1). Predicted with the SWISS-MODEL, there were two separate β-sandwich clusters linked with a high serine containing linker in ORF6. The N-terminal β-sandwich is a novel endoxylanase of the glycosyl hydrolase family 11 with a specific activity of 1150.00 U/mg. The optimal pH and temperature for this enzyme were shown to be pH 5.0 and 50°C, respectively. The C-terminal helped increase the stability of the xylanase but decreased the activity to some degree. The C-terminal β-sandwich could bind avicel, but no conserved domain could be found. It may be a novel carbohydrate-binding module.

  4. Managing Stability in the Taiwan Strait: Non-Military Policy towards Taiwan under Hu Jintao

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    Wu-ueh Chang

    2009-10-01

    Full Text Available China’s Taiwan policy has been one of coupling intimidation (the “stick” approach with coercion (the “carrot” approach, a policy mix which, in the near term, is not likely to change, as is evidenced by the passage of the “Anti-Secession Law” in March, 2005. However, under Hu Jintao, the focus has been on pragmatism. The warm atmosphere that presently reigns in the Taiwan Strait area is unprecedented. Further talks are expected before the two cross-Strait leaders are slated to step down, simultaneously, in 2012. An era of reconciliation and negotiations has dawned. For the first time there is consensus regarding norms of interaction between the two sides. Cross-Strait relations have stabilized after years of tumult. More open, stable and predictable cross-Strait relations are in the interests of both sides. Difficulties surely lie ahead, but they will be dealt with in a different manner than what has been witnessed in the past.

  5. Detection of a planetary system orbiting the eclipsing polar HU Aqr

    CERN Document Server

    Qian, S -B; Liao, W -P; Li, L -J; Zhu, L -Y; Dai, Z -B; He, J -J; Zhao, E -G; Zhang, J; Li, K

    2011-01-01

    Using the precise times of mid-egress of the eclipsing polar HU Aqr, we discovered that this polar is orbited by two or more giant planets. The two planets detected so far have masses of at least 5.9 and 4.5\\,M_{Jup}. Their respective distances from the polar are 3.6 AU and 5.4 AU with periods of 6.54 and 11.96 years, respectively. The observed rate of period decrease derived from the downward parabolic change in O-C curve is a factor 15 larger than the value expected for gravitational radiation. This indicates that it may be only a part of a long-period cyclic variation, revealing the presence of one more planet. It is interesting to note that the two detected circumbinary planets follow the Titus-Bode law of solar planets with n=5 and 6. We estimate that another 10 years of observations will reveal the presence of the predicted third planet.

  6. Construction of a eukaryotic expression plasmid pcDNA3.1-HuR-FLAG and its transient expression in NIH3T3 cells

    Directory of Open Access Journals (Sweden)

    Tao LI

    2011-04-01

    Full Text Available Objective To construct a eukaryotic expression vector for HuR and analyze its expression and biological function in NIH3T3 cells.Methods The total RNA was extracted from NIH3T3 cells and reverse transcribed to cDNAs.The coding region sequence of mouse HuR was then amplified by PCR and subcloned into the pcDNA3.1-FLAG plasmid.The recombinant plasmid pcDNA3.1-HuR-FLAG was verified by PCR and restriction endonuclease analysis,confirmed by DNA sequence analysis,and then transiently transfected into NIH3T3 cells with Lipofectamine LTX.The expression of HuR protein was determined by Western blotting,and the mRNA level of HuR and DUSP1 were analyzed by using real-time PCR.Result The recombinant plasmid pcDNA3.1-HuR-FLAG was correctly constructed.Twenty-four hours after transfection of the recombinant plasmid into NIH3T3 cells,the fusion protein was found to have highly expressed in the cells as revealed by Western blotting.Real-time PCR results detected that the over-expression of HuR could up-regulate the expression of DUSP1.Conclusion The eukaryotic expression vector for HuR-FLAG fusion protein has been successfully constructed and transiently expressed in NIH3T3 cells.It can be used in further analysis of the posttranscriptional regulation of DUSP1 by HuR in cancer cells.

  7. Massive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips.

    Energy Technology Data Exchange (ETDEWEB)

    Krylov, A. S.; Zasedateleva, O. A.; Prokopenko, D. V.; Rouviere-Yaniv, J.; Mirzabekov, A. D.; Biochip Technology Center; Engelhardt Inst. of Molecular Biology; Inst. de Biologie Physico-Chimique

    2001-06-15

    A generic hexadeoxyribonucleotide microchip has been applied to test the DNA-binding properties of HU histone-like bacterial protein, which is known to have a low sequence specificity. All 4096 hexamers flanked within 8mers by degenerate bases at both the 3'- and 5'-ends were immobilized within the 100 x 100 x 20 mm polyacrylamide gel pads of the microchip. Single-stranded immobilized oligonucleotides were converted in some experiments to the double-stranded form by hybridization with a specified mixture of 8mers. The DNA interaction with HU was characterized by three type of measurements: (i) binding of FITC-labeled HU to microchip oligonucleotides; (ii) melting curves of complexes of labeled HU with single-stranded microchip oligonucleotides; (iii) the effect of HU binding on melting curves of microchip double-stranded DNA labeled with another fluorescent dye, Texas Red. Large numbers of measurements of these parameters were carried out in parallel for all or many generic microchip elements in real time with a multi-wavelength fluorescence microscope. Statistical analysis of these data suggests some preference for HU binding to G/C-rich single-stranded oligonucleotides. HU complexes with double-stranded microchip 8mers can be divided into two groups in which HU binding either increased the melting temperature (T{sub m}) of duplexes or decreased it. The stabilized duplexes showed some preference for presence of the sequence motifs AAG, AGA and AAGA. In the second type of complex, enriched with A/T base pairs, the destabilization effect was higher for longer stretches of A/T duplexes. Binding of HU to labeled duplexes in the second type of complex caused some decrease in fluorescence. This decrease also correlates with the higher A/T content and lower T{sub m}. The results demonstrate that generic microchips could be an efficient approach in analysis of sequence specificity of proteins.

  8. Antidepressant effects of curcumin and HU-211 coencapsulated solid lipid nanoparticles against corticosterone-induced cellular and animal models of major depression

    Directory of Open Access Journals (Sweden)

    He XL

    2016-10-01

    Full Text Available Xiaolie He,* Yanjing Zhu,* Mei Wang, Guoxin Jing, Rongrong Zhu, Shilong Wang Research Center for Translational Medicine at East Hospital, School of Life Science and Technology, Tongji University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Major depression is a complex neuropsychiatric disorder with few treatment approaches. The use of nontargeted antidepressants induced many side effects with their low efficacy. A more precise targeting strategy is to develop nanotechnology-based drug delivery systems; hence, we employed solid lipid nanoparticles (SLNs to encapsulate HU-211 and curcumin (Cur. The antidepressant effects of the dual-drug nanoparticles (Cur/SLNs-HU-211 for major depression treatment were investigated in corticosterone-induced cellular and animal models of major depression. Cur/SLNs-HU-211 can effectively protect PC12 cells from corticosterone-induced apoptosis and can release more dopamine, which may be associated with the higher uptake of Cur/SLNs-HU-211 shown by cellular uptake behavior analysis. Additionally, Cur/SLNs-HU-211 significantly reduced the immobility time in forced swim test, enhanced fall latency in rotarod test, and improved the level of dopamine in mice blood. Cur/SLNs-HU-211 can deliver more Cur to the brain and thus produce a significant increase in neurotransmitters level in brain tissue, especially in the hippocampus and striatum. The results of Western blot and immunofluorescence revealed that Cur/SLNs-HU-211 can significantly enhance the expression of CB1, p-MEK1, and p-ERK1/2. Our study suggests that Cur/SLNs-HU-211 may have great potential for major depression treatment. Keywords: major depression, curcumin, HU-211, solid lipid nanoparticles, dopamine

  9. Cumulative approaches to track formation under swift heavy ion (SHI) irradiation: Phenomenological correlation with formation energies of Frenkel pairs

    Energy Technology Data Exchange (ETDEWEB)

    Crespillo, M.L., E-mail: mcrespil@utk.edu [Centro de Microanálisis de Materiales, CMAM-UAM, Cantoblanco, Madrid 28049 (Spain); Department of Materials Science & Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Agulló-López, F., E-mail: fal@uam.es [Centro de Microanálisis de Materiales, CMAM-UAM, Cantoblanco, Madrid 28049 (Spain); Zucchiatti, A. [Centro de Microanálisis de Materiales, CMAM-UAM, Cantoblanco, Madrid 28049 (Spain)

    2017-03-01

    Highlights: • Extensive survey formation energies Frenkel pairs and electronic stopping thresholds. • Correlation: track formation thresholds and the energies for Frenkel pair formation. • Formation energies Frenkel pairs discussed in relation to the cumulative mechanisms. • Amorphous track formation mechanisms: defect accumulation models versus melting. • Advantages cumulative models to deal with new hot topics: nuclear-electronic synergy. - Abstract: An extensive survey for the formation energies of Frenkel pairs, as representative candidates for radiation-induced point defects, is presented and discussed in relation to the cumulative mechanisms (CM) of track formation in dielectric materials under swift heavy ion (SHI) irradiation. These mechanisms rely on the generation and accumulation of point defects during irradiation followed by collapse of the lattice once a threshold defect concentration is reached. The physical basis of those approaches has been discussed by Fecht as a defect-assisted transition to an amorphous phase. Although a first quantitative analysis of the CM model was previously performed for LiNbO{sub 3} crystals, we have, here, adopted a broader phenomenological approach. It explores the correlation between track formation thresholds and the energies for Frenkel pair formation for a broad range of materials. It is concluded that the threshold stopping powers can be roughly scaled with the energies required to generate a critical Frenkel pair concentration in the order of a few percent of the total atomic content. Finally, a comparison with the predictions of the thermal spike model is discussed within the analytical Szenes approximation.

  10. SHiP: a new facility for searching for long-lived neutral particles and studying the tau neutrino properties

    CERN Document Server

    AUTHOR|(SzGeCERN)658186

    2016-01-01

    SHiP (Search for Hidden Particles) is a new general purpose fixed target facility, proposed at the CERN SPS accelerator. In its initial phase, the 400 GeV proton beam will be dumped on a heavy target, integrating $2\\times 10^{20}$ protons on target in 5 years. A dedicated detector located downstream of the target, based on a long vacuum tank and a spectrometer and particle identification detectors, will allow probing a variety of models with light long-lived neutral and very weakly interacting particles and masses below 10 GeV. The main focus will be the physics of the so-called Hidden Portals, i.e. search for Dark Photons, Light scalars and pseudo-scalars, and Heavy Neutrinos. The sensitivity to Heavy Neutrinos will allow for the first time to probe, in the mass range between the kaon and the charm meson mass, a coupling range for which the baryon asymmetry of the Universe could be explained. Direct detection of light and long-lived SUSY particles, such as RPV neutralinos and pseudo-Dirac gauginos could als...

  11. Cumulative approaches to track formation under swift heavy ion (SHI) irradiation: Phenomenological correlation with formation energies of Frenkel pairs

    Science.gov (United States)

    Crespillo, M. L.; Agulló-López, F.; Zucchiatti, A.

    2017-03-01

    An extensive survey for the formation energies of Frenkel pairs, as representative candidates for radiation-induced point defects, is presented and discussed in relation to the cumulative mechanisms (CM) of track formation in dielectric materials under swift heavy ion (SHI) irradiation. These mechanisms rely on the generation and accumulation of point defects during irradiation followed by collapse of the lattice once a threshold defect concentration is reached. The physical basis of those approaches has been discussed by Fecht as a defect-assisted transition to an amorphous phase. Although a first quantitative analysis of the CM model was previously performed for LiNbO3 crystals, we have, here, adopted a broader phenomenological approach. It explores the correlation between track formation thresholds and the energies for Frenkel pair formation for a broad range of materials. It is concluded that the threshold stopping powers can be roughly scaled with the energies required to generate a critical Frenkel pair concentration in the order of a few percent of the total atomic content. Finally, a comparison with the predictions of the thermal spike model is discussed within the analytical Szenes approximation.

  12. Chi Shi-Ying and the “Time and Tide” Magazine That Resisted the Trend(1959-1967)

    Directory of Open Access Journals (Sweden)

    Eve Chiu

    2015-04-01

    Full Text Available This thesis focuses on “Time and Tide” magazine that was published by Mr. Chi Shi-Ying during 1959 to 1967 in Taiwan, and how its content related to Chi’s critical political activities at that time. The plan is to clarify and marshal those main issues about democracy, legalization as well as press freedom on the one hand; therefore to reveal the riddle why Chi did became an opponent of KMT after being its faithful cadre for so many years. Chi devoted himself to the “New party-building” movement that against KMT, and was one of the pilot “wishenjen”(外省人) who got in the “Out of the party”(黨外) movement in Taiwan. Chi’s “Time and Tide” shared the same “structure of feeling” with the “Free China” published by Lei Zhen and with the readers of these two magazines. The “structure of feeling” once dived into the deep water of history, but is going to rise again after twenty years later.

  13. Mechanosensitivity below Ground: Touch-Sensitive Smell-Producing Roots in the Shy Plant Mimosa pudica1[OPEN

    Science.gov (United States)

    Musah, Rabi A.; Lesiak, Ashton D.; Maron, Max J.; Edwards, David; Fowble, Kristen L.; Long, Michael C.

    2016-01-01

    The roots of the shy plant Mimosa pudica emit a cocktail of small organic and inorganic sulfur compounds and reactive intermediates into the environment, including SO2, methanesulfinic acid, pyruvic acid, lactic acid, ethanesulfinic acid, propanesulfenic acid, 2-aminothiophenol, S-propyl propane 1-thiosulfinate, phenothiazine, and thioformaldehyde, an elusive and highly unstable compound that, to our knowledge, has never before been reported to be emitted by a plant. When soil around the roots is dislodged or when seedling roots are touched, an odor is detected. The perceived odor corresponds to the emission of higher amounts of propanesulfenic acid, 2-aminothiophenol, S-propyl propane 1-thiosulfinate, and phenothiazine. The mechanosensitivity response is selective. Whereas touching the roots with soil or human skin resulted in odor detection, agitating the roots with other materials such as glass did not induce a similar response. Light and electron microscopy studies of the roots revealed the presence of microscopic sac-like root protuberances. Elemental analysis of these projections by energy-dispersive x-ray spectroscopy revealed them to contain higher levels of K+ and Cl− compared with the surrounding tissue. Exposing the protuberances to stimuli that caused odor emission resulted in reductions in the levels of K+ and Cl− in the touched area. The mechanistic implications of the variety of sulfur compounds observed vis-à-vis the pathways for their formation are discussed. PMID:26661932

  14. The prose of Su Shi and the "book of changes"%苏轼散文与《周易》

    Institute of Scientific and Technical Information of China (English)

    杜佳悦

    2016-01-01

    苏轼旷达的人生态度不仅受佛、道思想影响,也受《周易》影响甚大。这种影响不仅体现在他所创作的《东坡易传》中,从苏轼的散文中,我们也可以发现,《周易》中所体现的文学思想与艺术上的特色对苏轼的散文创作也产生了重要影响。%Su Shi’s open-minded attitude toward life is not only influenced by Buddhism and Taoism, but also by the “book of changes”. This effect is reflected not only in his creation of “Dongpo Yi Zhuan”, but also in his prose. The literary thoughts and artistic characteristics in the “book of changes” has an important influence on Su Shi's prose.

  15. Effect of various factors and substrates on the growth of a human hepatoblastoma cell line, HuH-6 in a serum-free medium.

    Directory of Open Access Journals (Sweden)

    Tokiwa,Takayoshi

    1989-12-01

    Full Text Available The effect of various factors and substrates on the growth of a human hepatoblastoma cell line, HuH-6, which was inoculated at low density in a serum-free medium was examined. Several supplements were required to enhance cell growth of HuH-6. These included cholera toxin (CT, glucagon (Glu and selenium (Se. Type IV collagen (C-IV provided the most conductive environment tested for cell growth. These results suggest that CT, Glu, Se, and C-IV are important stimulators for the continuous growth of HuH-6 in a serum-free medium at low density.

  16. 师陀文学史定位的异质性%Heterogeneity of Shi Tuo’ s Orientation of History of Literature

    Institute of Scientific and Technical Information of China (English)

    叶淑钦

    2014-01-01

    文学史的叙述方式通常按照流派来纳入到文学史的整体叙述和阐释框架中,师陀的文学史定位一直引发研究者的争议和关注,左翼评论家与京派评论家都拒绝将其纳为本流派作家。通过对师陀在文学创作中的主题、风格、技巧等方面的分析,揭示师陀的作品具有主动疏离文坛文学流派地倾向,并细读师陀乡土小说主题等相似的文学作品,发现师陀的文学作品具有与他人完全不一样地艺术特色和内在意蕴,具有“奇特的风格”,从而呈现其文学史定位的异质性。%Narration form of history of literature is generally included into overall narration and interpretation framework according to school.Shi Tuo ’ s orientation of history of literature has always triggered dispute and atten-tion from researches.Both left -wing critics and Beijing -school critics reject including it into writers of this school.Through analysis of Shi Tuo ’ s theme, style and technique in literary creation , It reveals that the works of Shi Tuo have a tendency to initiatively be alienated from schools of literature of the literary world .And through reading Shi Tuo ’ s literary works similar to subjects such as local novels in details , it founds that his literary works have artistic characteristics and inherent connotation that are completely different from others and possess “peculiar style”, thus presenting heterogeneity of Shi Tuo ’ s literary history positioning.

  17. Identification of flavonoid diglycosides in several genotypes of asparagus from the Huétor-Tájar population variety.

    Science.gov (United States)

    Fuentes-Alventosa, José María; Rodríguez, Guillermo; Cermeño, Pedro; Jiménez, Ana; Guillén, Rafael; Fernández-Bolaños, Juan; Rodríguez-Arcos, Rocío

    2007-11-28

    The qualitative and quantitative composition of flavonoids from the Huétor-Tájar population variety of asparagus (commonly known as " triguero") was investigated. Flavonoids were analyzed by reversed-phase high-performance liquid chromatography-diode array detection (HPLC-DAD). Liquid chromatography-mass spectrometry (LC-MS) under identical HPLC conditions was used to verify the identities of the flavonoid glycosides from triguero asparagus. The quantities of asparagus flavonoids were calculated according to concentration curves constructed with authentic standards. Total flavonoid contents, calculated as the sum of individual compounds, were determined and ranged from 400 to 700 mg/kg fresh weight. The most abundant was rutin, which represented 55-98% of the total flavonoid complement. Triguero asparagus were revealed to be an important source of not only quercetin derivatives but also kaempferol and isorhamnetin glycosides. Significant differences (p asparagus genotypes from the Huétor-Tájar population variety.

  18. HTLV-1 Infection and Neuropathogenesis in the Context of Rag1(-/-)γc(-/-) (RAG1-Hu) and BLT Mice.

    Science.gov (United States)

    Ginwala, Rashida; Caruso, Breanna; Khan, Zafar K; Pattekar, Ajinkya; Chew, Glen M; Corley, Michael J; Loonawat, Ronak; Jacobson, Steven; Sreedhar, Sreesha; Ndhlovu, Lishomwa C; Jain, Pooja

    2017-09-01

    To date, the lack of a suitable small animal model has hindered our understanding of Human T-cell lymphotropic virus (HTLV)-1 chronic infection and associated neuropathogenesis defined as HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The host immune response plays a critical role in the outcome of HTLV-1 infection, which could be better tested in the context of humanized (hu) mice. Thus, we employ here the Balb/c-Rag1(-/-)γc(-/-) or Rag1 as well as Bone marrow-Liver-Thymic (BLT) mouse models for engraftment of human CD34(+) hematopoietic stem cells. Flow cytometry and histological analyses confirmed reconstitution of Rag1 and BLT mice with human immune cells. Following HTLV-1 infection, proviral load (PVL) was detected in the blood of Rag-1 and BLT hu-mice as early as 2 weeks post-infection (wpi) with sustained elevation in the subsequent weeks followed by Tax expression. Additionally, infection was compared between adult and neonatal Rag1 mice with both PVL and Tax expression considerably higher in the adult Rag1 mice as compared to the neonates. Establishment of peripheral infection led to lymphocytic infiltration with concomitant Tax expression and resulting myelin disruption within the central nervous system of infected mice. In addition, up-regulation in the expression of several immune checkpoint mediators such as programmed cell death-1 (PD-1), T-cell Ig and ITIM domain (TIGIT), and T cell Ig and mucin domain-3 protein (Tim-3) were observed on CD8(+) T cells in various organs including the CNS of infected hu-mice. Collectively, these studies represent the first attempt to establish HTLV-1 neuropathogenesis in the context of Rag-1 and BLT hu-mice as potential novel tools for understanding HTLV-1 neuropathogenesis and testing of novel therapies such as immune checkpoint blockade in the amelioration of chronic HTLV-1 infection.

  19. Comment on "Tropospheric temperature response to stratospheric ozone recovery in the 21st century" by Hu et al. (2011

    Directory of Open Access Journals (Sweden)

    C. McLandress

    2012-03-01

    Full Text Available In a recent paper Hu et al. (2011 suggest that the recovery of stratospheric ozone during the first half of this century will significantly enhance free tropospheric and surface warming caused by the anthropogenic increase of greenhouse gases, with the effects being most pronounced in Northern Hemisphere middle and high latitudes. These surprising results are based on a multi-model analysis of CMIP3 model simulations with and without prescribed stratospheric ozone recovery. Hu et al. suggest that in order to properly quantify the tropospheric and surface temperature response to stratospheric ozone recovery, it is necessary to run coupled atmosphere-ocean climate models with stratospheric ozone chemistry. The results of such an experiment are presented here, using a state-of-the-art chemistry-climate model coupled to a three-dimensional ocean model. In contrast to Hu et al., we find a much smaller Northern Hemisphere tropospheric temperature response to ozone recovery, which is of opposite sign. We suggest that their result is an artifact of the incomplete removal of the large effect of greenhouse gas warming between the two different sets of models.

  20. Cannabinoid HU210 protects isolated rat stomach against impairment caused by serum of rats with experimental acute pancreatitis.

    Directory of Open Access Journals (Sweden)

    Ming-hua Cao

    Full Text Available Acute pancreatitis (AP, especially severe acute pancreatitis often causes extra-pancreatic complications, such as acute gastrointestinal mucosal lesion (AGML which is accompanied by a considerably high mortality, yet the pathogenesis of AP-induced AGML is still not fully understood. In this report, we investigated the alterations of serum components and gastric endocrine and exocrine functions in rats with experimental acute pancreatitis, and studied the possible contributions of these alterations in the pathogenesis of AGML. In addition, we explored the intervention effects of cannabinoid receptor agonist HU210 and antagonist AM251 on isolated and serum-perfused rat stomach. Our results showed that the AGML occurred after 5 h of AP replication, and the body homeostasis was disturbed in AP rat, with increased levels of pancreatic enzymes, lipopolysaccharide (LPS, proinflammtory cytokines and chemokines in the blood, and an imbalance of the gastric secretion function. Perfusing the isolated rat stomach with the AP rat serum caused morphological changes in the stomach, accompanied with a significant increment of pepsin and [H+] release, and increased gastrin and decreased somatostatin secretion. HU210 reversed the AP-serum-induced rat pathological alterations, including the reversal of transformation of the gastric morphology to certain degree. The results from this study prove that the inflammatory responses and the imbalance of the gastric secretion during the development of AP are responsible for the pathogenesis of AGML, and suggest the therapeutic potential of HU210 for AGML associated with acute pancreatitis.

  1. Alginate hydrogel protects encapsulated hepatic HuH-7 cells against hepatitis C virus and other viral infections.

    Directory of Open Access Journals (Sweden)

    Nhu-Mai Tran

    Full Text Available Cell microencapsulation in alginate hydrogel has shown interesting applications in regenerative medicine and the biomedical field through implantation of encapsulated tissue or for bioartificial organ development. Although alginate solution is known to have low antiviral activity, the same property regarding alginate gel has not yet been studied. The aim of this work is to investigate the potential protective effect of alginate encapsulation against hepatitis C virus (HCV infection for a hepatic cell line (HuH-7 normally permissive to the virus. Our results showed that alginate hydrogel protects HuH-7 cells against HCV when the supernatant was loaded with HCV. In addition, alginate hydrogel blocked HCV particle release out of the beads when the HuH-7 cells were previously infected and encapsulated. There was evidence of interaction between the molecules of alginate hydrogel and HCV, which was dose- and incubation time-dependent. The protective efficiency of alginate hydrogel towards HCV infection was confirmed against a variety of viruses, whether or not they were enveloped. This promising interaction between an alginate matrix and viruses, whose chemical mechanisms are discussed, is of great interest for further medical therapeutic applications based on tissue engineering.

  2. Chemopreventive apigenin controls UVB-induced cutaneous proliferation and angiogenesis through HuR and thrombospondin-1.

    Science.gov (United States)

    Tong, Xin; Mirzoeva, Salida; Veliceasa, Dorina; Bridgeman, Bryan B; Fitchev, Philip; Cornwell, Mona L; Crawford, Susan E; Pelling, Jill C; Volpert, Olga V

    2014-11-30

    Plant flavonoid apigenin prevents and inhibits UVB-induced carcinogenesis in the skin and has strong anti-proliferative and anti-angiogenic properties. Here we identify mechanisms, by which apigenin controls these oncogenic events. We show that apigenin acts, at least in part, via endogenous angiogenesis inhibitor, thrombospondin-1 (TSP1). TSP1 expression by the epidermal keratinocytes is potently inhibited by UVB. It inhibits cutaneous angiogenesis and UVB-induced carcinogenesis. We show that apigenin restores TSP1 in epidermal keratinocytes subjected to UVB and normalizes proliferation and angiogenesis in UVB-exposed skin. Importantly, reconstituting TSP1 anti-angiogenic function in UVB-irradiated skin with a short bioactive peptide mimetic representing exclusively its anti-angiogenic domain reproduced the anti-proliferative and anti-angiogenic effects of apigenin. Cox-2 and HIF-1α are important mediators of angiogenesis. Both apigenin and TSP1 peptide mimetic attenuated their induction by UVB. Finally we identified the molecular mechanism, whereby apigenin did not affect TSP1 mRNA, but increased de novo protein synthesis. Knockdown studies implicated the RNA-binding protein HuR, which controls mRNA stability and translation. Apigenin increased HuR cytoplasmic localization and physical association with TSP1 mRNA causing de novo TSP1 synthesis. HuR cytoplasmic localization was, in turn, dependent on CHK2 kinase. Together, our data provide a new mechanism, by which apigenin controls UVB-induced carcinogenesis.

  3. Cannabinoid HU210 Protects Isolated Rat Stomach against Impairment Caused by Serum of Rats with Experimental Acute Pancreatitis

    Science.gov (United States)

    Cao, Ming-hua; Li, Yong-yu; Xu, Jing; Feng, Ya-jing; Lin, Xu-hong; Li, Kun; Han, Tong; Chen, Chang-Jie

    2012-01-01

    Acute pancreatitis (AP), especially severe acute pancreatitis often causes extra-pancreatic complications, such as acute gastrointestinal mucosal lesion (AGML) which is accompanied by a considerably high mortality, yet the pathogenesis of AP-induced AGML is still not fully understood. In this report, we investigated the alterations of serum components and gastric endocrine and exocrine functions in rats with experimental acute pancreatitis, and studied the possible contributions of these alterations in the pathogenesis of AGML. In addition, we explored the intervention effects of cannabinoid receptor agonist HU210 and antagonist AM251 on isolated and serum-perfused rat stomach. Our results showed that the AGML occurred after 5 h of AP replication, and the body homeostasis was disturbed in AP rat, with increased levels of pancreatic enzymes, lipopolysaccharide (LPS), proinflammtory cytokines and chemokines in the blood, and an imbalance of the gastric secretion function. Perfusing the isolated rat stomach with the AP rat serum caused morphological changes in the stomach, accompanied with a significant increment of pepsin and [H+] release, and increased gastrin and decreased somatostatin secretion. HU210 reversed the AP-serum-induced rat pathological alterations, including the reversal of transformation of the gastric morphology to certain degree. The results from this study prove that the inflammatory responses and the imbalance of the gastric secretion during the development of AP are responsible for the pathogenesis of AGML, and suggest the therapeutic potential of HU210 for AGML associated with acute pancreatitis. PMID:23285225

  4. Effect of route of infection on outcome of Toxoplasma gondii infection in hu-PBL SCID mice.

    Science.gov (United States)

    Meyer, Desiree J; Allan, Jane E; Beaman, Miles H

    2013-03-01

    Toxoplasma gondii (T. gondii) causes serious infection, especially in immunocompromised hosts. The relevance of animal models of toxoplasmosis to human disease is unclear, but have indicated that the route of Toxoplasma infection may affect the outcome. A humanized model of toxoplasmosis of immunocompromised mice (i.e. hu-PBL SCID), using the intraperitoneal (IP) route demonstrated long-term engraftment of human cells and worsening of inflammation compared to controls. In this study, we examined the effect of route of infection on this hu-PBL SCID model using a Toxoplasma strain (i.e. DAG) isolated from an immunocompromised human. Oral infection led to an asymptomatic infection, whereas animals infected by the IP route succumbed more quickly to infection. Human cells, detected through species-specific β-actin mRNA, were not as prominent in IP-infected animals as compared to orally infected and uninfected animals. There was evidence of control of toxoplasmosis in some orally infected animals, and this was associated with the presence of human cells in multiple tissues. Thus, the route of infection dramatically affects the outcome of infection, either by affecting parasite replication or expansion of human immune cells. Further studies of oral Toxoplasma infection using hu-PBL SCID mice may help in developing chemotherapies and immunotherapeutic strategies for toxoplasmosis.

  5. A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

    Directory of Open Access Journals (Sweden)

    Zhaoyang Zhang

    2011-11-01

    Full Text Available We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α. In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor, on the surface of which the complementary sequences are linked (as cODN-AuNPs and pre-hybridized with carboxymethylfluorescein (FAM-labeled anti-rHuEPO-α aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-α concentrations as low as 0.92 nM within a few hours.

  6. Comment on "Tropospheric temperature response to stratospheric ozone recovery in the 21st century" by Hu et al. (2011

    Directory of Open Access Journals (Sweden)

    M. Previdi

    2012-01-01

    Full Text Available Stratospheric ozone recovery is expected to figure prominently in twenty-first century climate change. In a recent paper, Hu et al. (2011 argue that one impact of ozone recovery will be to enhance the warming of the surface-troposphere system produced by increases in well-mixed greenhouse gases; furthermore, this enhanced warming would be strongest in the Northern Hemisphere, which is surprising since previous studies have consistently shown the effects of stratospheric ozone changes to be most pronounced in the Southern Hemisphere. Hu et al. (2011 base their claims largely on differences in the simulated temperature change between two groups of IPCC climate models, one group which included stratospheric ozone recovery in its twenty-first century simulations and a second group which did not. Both groups of models were forced with the same increases in well-mixed greenhouse gases according to the A1B emissions scenario. In the current work, we compare the surface temperature responses of the same two groups of models in a different experiment in which atmospheric CO2 was increased by 1% per year until doubling. We find remarkably similar differences in the simulated surface temperature change between the two sets of models as Hu et al. (2011 found for the A1B experiment, suggesting that the enhanced warming which they attribute to stratospheric ozone recovery is actually a reflection of different responses of the two model groups to greenhouse gas forcing.

  7. Morphological and kinematic signatures of a binary central star in the planetary nebula Hu 2-1

    CERN Document Server

    Miranda, L F; Guerrero, M; Vázquez, R A; Gómez, Y; Miranda, Luis F.; Torrelles, Jose M.; Guerrero, Martin A.; Vazquez, Roberto; Gomez, Yolanda

    2000-01-01

    We present narrow-band optical images, VLA-A 3.6 cm continuum and H92alpha emission line data and high resolution long-slit [N II] spectra of Hu 2-1. The formation of Hu 2-1 appears dominated by anisotropic mass ejection during the late AGB stage of its progenitor and by variable, ``precessing'' collimated bipolar outflows during the proto-PN and/or early PN phases. The data strongly support the existence of a binary central star at center of Hu 2-1: (1) the brightness distribution of the bipolar lobes and inner shell is point-symmetric, and the bipolar lobes show departures from axial symmetry; (2) the position of the central star is off-center; (3) mass ejection towards the equatorial plane is detected; (4) ``precessing'' collimated outflows are present. Besides all this circumstantial evidences, the analysis of the kinematics shows that the systemic velocity of the bipolar outflows does not coincide with the systemic velocity of the nebula. We propose that this velocity difference is a direct evidence of o...

  8. Modeling and predicting hemorrhagic fever with renal syndrome trends based on meteorological factors in Hu County, China.

    Directory of Open Access Journals (Sweden)

    Dan Xiao

    Full Text Available Hu County is a serious hemorrhagic fever with renal syndrome (HFRS epidemic area, with notable fluctuation of the HFRS epidemic in recent years. This study aimed to explore the optimal model for HFRS epidemic prediction in Hu.Three models were constructed and compared, including a generalized linear model (GLM, a generalized additive model (GAM, and a principal components regression model (PCRM. The fitting and predictive adjusted R2 of each model were calculated. Ljung-Box Q tests for fitted and predicted residuals of each model were conducted. The study period was stratified into before (1971-1993 and after (1994-2012 vaccine implementation epochs to avoid the confounding factor of vaccination.The autocorrelation of fitted and predicted residuals of the GAM in the two epochs were not significant (Ljung-Box Q test, P>.05. The adjusted R2 for the predictive abilities of the GLM, GAM, and PCRM were 0.752, 0.799, and 0.665 in the early epoch, and 0.669, 0.756, and 0.574 in the recent epoch. The adjusted R2 values of the three models were lower in the early epoch than in the recent epoch.GAM is superior to GLM and PCRM for monthly HFRS case number prediction in Hu County. A shift in model reliability coincident with vaccination implementation demonstrates the importance of vaccination in HFRS control and prevention.

  9. Orally administrated Juzen-taiho-to/TJ-48 ameliorates erythropoietin (rHuEPO)-resistant anemia in patients on hemodialysis.

    Science.gov (United States)

    Nakamoto, Hidetomo; Mimura, Taku; Honda, Nobuko

    2008-10-01

    Maintenance of the red blood cell volume is a fundamental aspect of ensuring oxygen supply to the tissue. Recombinant human erythropoietin (rHuEPO) was approved for marketing in Japan in 1990 for the treatment of anemia in patients on dialysis. Recombinant human erythropoietin caused a significant increase in hemoglobin (Hb) levels in patients on dialysis. However, not all have a good response to rHuEPO therapy; the causes of rHuEPO failure include iron deficiency, infection, uremia, and interaction of some drugs. Juzen-taiho-to (TJ-48), a mixture of extracts from 10 medicinal herbs, has been used traditionally to treat patients with anemia, anorexia, or fatigue. To clarify the effect of TJ-48 on erythropoietin-resistant anemia, we studied the effect of TJ-48 in patients on hemodialysis with erythropoietin-resistant anemia. We divided 42 end-stage renal disease patients on hemodialysis with erythropoietin-resistant anemia (Hbrenal disease patients. This effect was, at least in part, due to the anti-inflammatory effect of TJ-48 in patients on hemodialysis.

  10. Leukemia SH-1 Cells Purged by ZnPcH1-Based Photodynamic Therapy%ZnPcH1介导的光动力疗法净化白血病SHI-1细胞

    Institute of Scientific and Technical Information of China (English)

    林晓岚; 黄慧芳; 陈万紫

    2012-01-01

    本研究探讨新型酞菁类光敏剂二磺基二邻苯二甲酰亚胺甲基酞菁锌(ZnPcH1)介导的光动力疗法(PDT)对急性单核细胞白血病SHI-1细胞的杀伤作用及其机制,为PDT应用于白血病自体骨髓体外净化提供理论依据.以SHI-1细胞作为研究对象,应用MTT比色法检测细胞增殖.采用AO/EB复合染色、TUNEL、DNA二倍体分析、Annexin-V -FITC/PI双染法等分析细胞的死亡方式.SHI-1细胞与正常骨髓单个核细胞(MNC)以1∶100-1∶10000混合建立混合细胞模型,通过巢式RT-PCR检测SHI-1细胞融合基因MLL/AF6 mRNA的表达以研究ZnPcH1-PDT对骨髓MNC中所掺入的SHI-1细胞的净化效果.结果表明,ZnPcH1-PDT对SHI-1细胞有杀伤作用,且呈量效关系.ZnPcH1-PDT能诱导细胞凋亡,且呈时间依赖性.0.5 μmol/L ZnPcH1-PDT可完全杀灭模拟缓解骨髓中掺入的SHI-1细胞.结论:ZnPcH1-PDT有希望成为新的高效而简便的骨髓净化手段.%The objective of this study was to investigate the effect of a novel Zinc phthalocyanine (ZnPcH1 ) based photodynamic therapy (PDT) on acute monocytic leukemia cell lines SHI-1 and its mechanism, so as to provide theory basis for bone marrow purging in vino for patients with leukemia. The killing effect of ZnPcH, -PDT on SHI-1 cells were assessed by MTT method; the SHI-1 cell death patterns were analyzed by AO/EB fluorescence staining, TdT-mediated dUTP nick end labeling (TUNEL), DNA ploidy analysis,and Annexin V-FITC/P1 double staining. Cell mixture was established by integrating SHI-1 cells with normal bone marrow MNC ( by 1:100 - 1:10 000). Purging effect of ZnPcH1-PDT against SHI-1 mixed into normal MNC was assessed by analyzing the expression of fusion gene MLL/AF6 mRNA using nested RT-PCR. The results showed that ZnPcH, -PDT could effectively inhibit SHI-1 cell proliferation in dose-dependent manner, and ZnPcH, -PDT could induce cell apoptosis in time-dependent manner. 0.5 μmol/L ZnPcH, -PDT could completely

  11. Bone and muscle assessment in patients undergoing total hip arthroplasty using HU based analysis

    Directory of Open Access Journals (Sweden)

    Þröstur Pétursson

    2012-09-01

    Full Text Available Total hip arthroplasty (THA is performed with or without the use of bone cement. The lack of reliable clinical guidelines for deciding which one to implement has encouraged this approach of joint clinical and engineering with the following objectives: 1. Validate quadriceps muscles and femur bone atrophy by extracting the mineral density from Computer Tomographic (CT images. 2. Validate computational processes based on 3-D modeling and Finite Element Methods (FEM. A clinical trial was started, where 36 volunteer patients underwent THA surgery for the first time: 18 receiving cemented implant and 18 receiving uncemented implant. The patients were CT scanned prior-, immediately after and 12 months post-surgery. The CT data are further processed to segment muscles and bones and to create 3D-models for the simulation and for calculating bone mineral density (BMD. Furthermore quadriceps muscle density Hounsfield (HU based value is calculated from the segmented file on healthy and operated leg. These preliminary results indicate computational tools and methods that are able to quantitatively analyse patient’s condition pre and post-surgery. The BMD and muscle density measurement in correlation with the fracture risk analysis display a potential method for eligibility to receive non-cemented implant; the preliminary results show that also elderly that according with current clinical evaluation receives a cemented implant are suitable for the non-cemented type. The risk for structural failure during THA surgery is estimated by calculating femoral bone fracture risk index (FRI as a ratio between compressive stress during surgery and estimated failure stress on bone. The correlations with the BMD observations during the clinical trial will assess and validate this potential predictor tool.

  12. Publicidad y consumo de alimentos en estudiantes de Huánuco (Perú

    Directory of Open Access Journals (Sweden)

    María Luisa Sevillano García

    2012-10-01

    Full Text Available Las importantes carencias nutritivas apreciadas en estudiantes del distrito de Huánuco (Perú, junto con la publicidad televisiva de productos de dudosa calidad nutricional observada, nos llevó a plantear y desarrollar una investigación con el objeto de analizar la relación entre ambos fenómenos titulada: Influencia de los anuncios televisivos en el consumo de alimentos publicitados durante el 2010, cuyo resumen se presenta en este artículo. En la investigación planteamos un estudio correlacional con una muestra de 120 escolares de diversas instituciones educativas, a quienes se aplicaron satisfactoriamente encuestas, entrevistas y guía de evaluación física; también se analizó la publicidad emitida por los canales de televisión sobre alimentos y su contenido nutricional. El análisis inferencial aplicado fue Ji cuadrado (?2. Con esta investigación se ha demostrado que el estado de salud de los escolares está afectado, siendo muy agudo respecto a la caries dental, problema que se atribuye al consumo de ciertos alimentos. Se constata que existe relación positiva, con alta significancia estadística, entre la adherencia a los anuncios televisivos y el consumo de alimentos publicitados de alto contenido en sodio, calcio, azúcar y de calorías por los escolares estudiados; así mismo, se prueba la interdependencia entre la exposición a los anuncios televisivos y el consumo de alimentos con productos de alto contenido en grasa que merecen ser vigilados y controlados por los responsables.

  13. Benchmark critical experiments on low-enriched uranium oxide systems with H/U = 0. 77

    Energy Technology Data Exchange (ETDEWEB)

    Tuck, G; Oh, I

    1979-08-01

    Ten benchmark experiments were performed at the Critical Mass Laboratory at Rockwell International's Rocky Flats Plant, Golden, Colorado, for the US Nuclear Regulatory Commission. They provide accurate criticality data for low-enriched damp uranium oxide (U/sub 3/O/sub 8/) systems. The core studied consisted of 152 mm cubical aluminum cans containing an average of 15,129 g of low-enriched (4.46% /sup 235/U) uranium oxide compacted to a density of 4.68 g/cm/sup 3/ and with an H/U atomic ratio of 0.77. One hundred twenty five (125) of these cans were arranged in an approx. 770 mm cubical array. Since the oxide alone cannot be made critical in an array of this size, an enriched (approx. 93% /sup 235/U) metal or solution driver was used to achieve criticality. Measurements are reported for systems having the least practical reflection and for systems reflected by approx. 254-mm-thick concrete or plastic. Under the three reflection conditions, the mass of the uranium metal driver ranged from 29.87 kg to 33.54 kg for an oxide core of 1864.6 kg. For an oxide core of 1824.9 kg, the weight of the high concentration (351.2 kg U/m/sup 3/) solution driver varied from 14.07 kg to 16.14 kg, and the weight of the low concentration (86.4 kg U/m/sup 3/) solution driver from 12.4 kg to 14.0 kg.

  14. HuMiTar: A sequence-based method for prediction of human microRNA targets

    Directory of Open Access Journals (Sweden)

    Chen Ke

    2008-12-01

    Full Text Available Abstract Background MicroRNAs (miRs are small noncoding RNAs that bind to complementary/partially complementary sites in the 3' untranslated regions of target genes to regulate protein production of the target transcript and to induce mRNA degradation or mRNA cleavage. The ability to perform accurate, high-throughput identification of physiologically active miR targets would enable functional characterization of individual miRs. Current target prediction methods include traditional approaches that are based on specific base-pairing rules in the miR's seed region and implementation of cross-species conservation of the target site, and machine learning (ML methods that explore patterns that contrast true and false miR-mRNA duplexes. However, in the case of the traditional methods research shows that some seed region matches that are conserved are false positives and that some of the experimentally validated target sites are not conserved. Results We present HuMiTar, a computational method for identifying common targets of miRs, which is based on a scoring function that considers base-pairing for both seed and non-seed positions for human miR-mRNA duplexes. Our design shows that certain non-seed miR nucleotides, such as 14, 18, 13, 11, and 17, are characterized by a strong bias towards formation of Watson-Crick pairing. We contrasted HuMiTar with several representative competing methods on two sets of human miR targets and a set of ten glioblastoma oncogenes. Comparison with the two best performing traditional methods, PicTar and TargetScanS, and a representative ML method that considers the non-seed positions, NBmiRTar, shows that HuMiTar predictions include majority of the predictions of the other three methods. At the same time, the proposed method is also capable of finding more true positive targets as a trade-off for an increased number of predictions. Genome-wide predictions show that the proposed method is characterized by 1.99 signal

  15. miR-29b, miR-205 and miR-221 enhance chemosensitivity to gemcitabine in HuH28 human cholangiocarcinoma cells.

    Directory of Open Access Journals (Sweden)

    Kinya Okamoto

    Full Text Available BACKGROUND AND AIMS: Cholangiocarcinoma (CCA is highly resistant to chemotherapy, including gemcitabine (Gem treatment. MicroRNAs (miRNAs are endogenous, non-coding, short RNAs that can regulate multiple genes expression. Some miRNAs play important roles in the chemosensitivity of tumors. Here, we examined the relationship between miRNA expression and the sensitivity of CCA cells to Gem. METHODS: Microarray analysis was used to determine the miRNA expression profiles of two CCA cell lines, HuH28 and HuCCT1. To determine the effect of candidate miRNAs on Gem sensitivity, expression of each candidate miRNA was modified via either transfection of a miRNA mimic or transfection of an anti-oligonucleotide. Ontology-based programs were used to identify potential target genes of candidate miRNAs that were confirmed to affect the Gem sensitivity of CCA cells. RESULTS: HuCCT1 cells were more sensitive to Gem than were HuH28 cells, and 18 miRNAs were differentially expressed whose ratios over ± 2log2 between HuH28 and HuCCT1. Among these 18 miRNAs, ectopic overexpression of each of three downregulated miRNAs in HuH28 (miR-29b, miR-205, miR-221 restored Gem sensitivity to HuH28. Suppression of one upregulated miRNA in HuH28, miR-125a-5p, inhibited HuH28 cell proliferation independently to Gem treatment. Selective siRNA-mediated downregulation of either of two software-predicted targets, PIK3R1 (target of miR-29b and miR-221 or MMP-2 (target of miR-29b, also conferred Gem sensitivity to HuH28. CONCLUSIONS: miRNA expression profiling was used to identify key miRNAs that regulate Gem sensitivity in CCA cells, and software that predicts miRNA targets was used to identify promising target genes for anti-tumor therapies.

  16. MiR-291b-3p Induces Apoptosis in Liver Cell Line NCTC1469 by Reducing the Level of RNA-binding Protein HuR

    Directory of Open Access Journals (Sweden)

    Jun Guo

    2014-03-01

    Full Text Available Background: There is increasing evidence that miRNAs are involved in cellular apoptosis. However, the specific role of miR-291b-3p in apoptosis has not been elucidated. In the present study, we investigated the effect of miR-291b-3p on NCTC1469 cell growth and apoptosis. Methods: Cell viability and apoptosis were examined in NCTC1469 cells transfected with miR-291b-3p mimics, inhibitor miRNA or negative control. Using computational miRNA target prediction databases, HuR was predicted as a target of miR-291b-3p. Luciferase assay, immunofluorescence and western blot were used to further explore the effects of miR-291b-3p on HuR expression. In addition, the effect of HuR on cell apoptosis was evaluated using a HuR-specific siRNA. Results: TNF-α-induced hepatocyte apoptosis was accompanied by enhanced expression of miR-291b-3p, suggesting that miR-291b-3p might contribute to the apoptotic process. Follow-up experiments showed that upregulation of miR-291b-3p decreased cell viability and induced NCTC1469 cell apoptosis. Additionally, similar to the activity of miR-519, which is another member of the same miRNA family, miR-291b-3p suppressed HuR translation through binding to the HuR coding region (CR. We further showed that the downregulation of HuR expression by miR-291b-3p was accompanied by reduced Bcl-2 expression. Moreover, knockdown of HuR also impaired Bcl-2 expression and increased the ratio of Bax/Bcl-2. More significantly, downregulation of miR-291b-3p failed to increase Bcl2 expression in NCTC1469 cells that were co-transfected with siRNA-HuR. Finally, inhibition of miR-291b-3p led to reduced apoptosis, while knockdown of HuR by siRNA promoted apoptosis, even in NCTC1469 cells that were co-transfected with the miR-291b-3p inhibitor. Conclusion: The current data suggested that miR-291b-3p contributed to NCTC1469 cell apoptosis by regulating the expression of HuR, which in turn increased Bcl-2 stability.

  17. PKCε promotes HuD-mediated neprilysin mRNA stability and enhances neprilysin-induced Aβ degradation in brain neurons.

    Directory of Open Access Journals (Sweden)

    Chol Seung Lim

    Full Text Available Amyloid-beta (Aβ peptide accumulation in the brain is a pathological hallmark of all forms of Alzheimer's disease. An imbalance between Aβ production and clearance from the brain may contribute to accumulation of neurotoxic Aβ and subsequent synaptic loss, which is the strongest correlate of the extent of memory loss in AD. The activity of neprilysin (NEP, a potent Aβ-degrading enzyme, is decreased in the AD brain. Expression of HuD, an mRNA-binding protein important for synaptogenesis and neuronal plasticity, is also decreased in the AD brain. HuD is regulated by protein kinase Cε (PKCε, and we previously demonstrated that PKCε activation decreases Aβ levels. We hypothesized that PKCε acts through HuD to stabilize NEP mRNA, modulate its localization, and support NEP activity. Conversely, loss of PKCε-activated HuD in AD leads to decreased NEP activity and accumulation of Aβ. Here we show that HuD is associated with NEP mRNA in cultures of human SK-N-SH cells. Treatment with bryostatin, a PKCε-selective activator, enhanced NEP association with HuD and increased NEP mRNA stability. Activation of PKCε also increased NEP protein levels, increased NEP phosphorylation, and induced cell surface expression. In addition, specific PKCε activation directly stimulated NEP activity, leading to degradation of a monomeric form of Aβ peptide and decreased Aβ neuronal toxicity, as measured by cell viability. Bryostatin treatment also rescued Aβ-mediated inhibition of HuD-NEP mRNA binding, NEP protein expression, and NEP cell membrane translocation. These results suggest that PKCε activation reduces Aβ by up-regulating, via the mRNA-binding protein HuD, Aβ-degrading enzymes such as NEP. Thus, PKCε activation may have therapeutic efficacy for AD by reducing neurotoxic Aβ accumulation as well as having direct anti-apoptotic and synaptogenic effects.

  18. President Hu is in India but Who Am I in China?%胡主席在印度,我却在中国

    Institute of Scientific and Technical Information of China (English)

    Rajesh Kanoi (Jack)

    2007-01-01

    @@ Today, Sunday morning, I sit thinking and typing at my computer. My thoughts are in my homeland, India and my body is in China. The body is here and mind there for a good reason. Mr. Hu is president and I am resident and both are in each other's countries (what a difference the absence of one small letter makes!). Mr. Hu will return in a few days and I will stay on longer.

  19. HuGE Watch: tracking trends and patterns of published studies of genetic association and human genome epidemiology in near-real time.

    Science.gov (United States)

    Yu, Wei; Wulf, Anja; Yesupriya, Ajay; Clyne, Melinda; Khoury, Muin Joseph; Gwinn, Marta

    2008-09-01

    HuGE Watch is a web-based application for tracking the evolution of published studies on genetic association and human genome epidemiology in near-real time. The application allows users to display temporal trends and spatial distributions as line charts and google maps, providing a quick overview of progress in the field. http://www.hugenavigator.net/HuGENavigator/startPageWatch.do

  20. The Art of Writing of Su Shi's Poetry%苏轼词的辞章艺术

    Institute of Scientific and Technical Information of China (English)

    宋贝贝; 周红海

    2013-01-01

      当代汉语辞章学属于汉语研究的新领域,属于广义上的修辞学。它是一门具有民族性、融合性和桥梁性的学科。尤其是辞章章法学,作为辞章学的一个分支,成就最高,具有很强的实用性。本文立足于辞章章法学这一崭新的角度上,对苏轼词的结构进行了深刻的剖析,针对苏词中运用最多的调和类章法和中性类章法,进行了较为详尽的阐释,从而认识宋词在谋篇布局上体现出的高度艺术价值。%Contemporary Chinese study of the art of writing belongs to the new field of Chinese studies , and belongs to the broad rhetoric.It is a national, inclusive and connective discipline .The study of the art of writing, as a branch of Rhetoric, makes the highest achievement and has very strong practicability .This article analyzes the structure of Su Shi's poetry in a new view of the study of the art of writing, elaborates the harmonic and neutral art of composition .Therefore, we can recognize the high artistic value of the rules and layout of Song Poems .

  1. Neutron activation analysis of sources of raw material of Emperor Qin Shi Huang's Terracotta Warriors and Horses

    Institute of Scientific and Technical Information of China (English)

    高正耀; 赵维娟; 李国霞; 谢建忠; 韩国河; 冯松林; 范东宇; 张颖; 柴之芳; 李融武; 张仲立; 朱君孝

    2003-01-01

    There have been selected 83 samples of terracotta warriors and horses of Emperor Qin Shi Huang's Mausoleum, 20 samples of clays taken from around Qin's Mausoleum and 2 samples of Yaozhou porcelain bodies. All these samples have been measured by instrument neutron activation analysis (INAA) and as many as 32 kinds of element contents of each sample are measured. The following conclusion has been reached when fuzzy cluster analysis is conducted to element contents of all these samples: (i) The samples are roughly classified into five categories: namely, samples from pits No. 1 and No. 2; samples from pit No. 3; loam layers; the mixture of loam and loess; and Yaozhou porcelain bodies. (ii) The terracottawarriors and horses in pits No. 1, No. 2 and No. 3 are relatively independent from one another. The clays from which they were made are not entirely identical.We have found that samples in pit No. 3 are very closely related and their claysources are comparatively concentrated. Samples in pits No. 1 and No. 2 are less related and their clay sources are comparatively scattered. (iii) The clays from which the terracotta warriors and horses were made are closely related to theloam layer near Qin's Mausoleum, particularly to the loam layer of Zaoyuan village and Gaoxing village, but they are not so related to loess layers there, nor to the loam layers of Anhoubao, even less related to Yaozhou porcelain bodies. Arational deduction thus drawn is that the raw material of clays from which the terracotta warriors and horses were made might probably be taken from loam layers around Zaoyuan and Gaoxing, or loam layers near Qin's Mausoleum whose properties are identical with those of loam layers of Zaoyuan and Gaoxing, rather than loess layers around the above places. Since the raw material of the terracotta warriors and horses was taken from loam near Qin's Mausoleum, it could be deductedthat the kiln sites might be located in around Qin's Mausoleum.

  2. RNA-binding protein HuR sequesters microRNA-21 to prevent translation repression of proinflammatory tumor suppressor gene programmed cell death 4.

    Science.gov (United States)

    Poria, D K; Guha, A; Nandi, I; Ray, P S

    2016-03-31

    Translation control of proinflammatory genes has a crucial role in regulating the inflammatory response and preventing chronic inflammation, including a transition to cancer. The proinflammatory tumor suppressor protein programmed cell death 4 (PDCD4) is important for maintaining the balance between inflammation and tumorigenesis. PDCD4 messenger RNA translation is inhibited by the oncogenic microRNA, miR-21. AU-rich element-binding protein HuR was found to interact with the PDCD4 3'-untranslated region (UTR) and prevent miR-21-mediated repression of PDCD4 translation. Cells stably expressing miR-21 showed higher proliferation and reduced apoptosis, which was reversed by HuR expression. Inflammatory stimulus caused nuclear-cytoplasmic relocalization of HuR, reversing the translation repression of PDCD4. Unprecedentedly, HuR was also found to bind to miR-21 directly, preventing its interaction with the PDCD4 3'-UTR, thereby preventing the translation repression of PDCD4. This suggests that HuR might act as a 'miRNA sponge' to regulate miRNA-mediated translation regulation under conditions of stress-induced nuclear-cytoplasmic translocation of HuR, which would allow fine-tuned gene expression in complex regulatory environments.

  3. Changes in Cellular mRNA Stability, Splicing, and Polyadenylation through HuR Protein Sequestration by a Cytoplasmic RNA Virus

    Directory of Open Access Journals (Sweden)

    Michael D. Barnhart

    2013-11-01

    Full Text Available The impact of RNA viruses on the posttranscriptional regulation of cellular gene expression is unclear. Sindbis virus causes a dramatic relocalization of the cellular HuR protein from the nucleus to the cytoplasm in infected cells. This is to the result of the expression of large amounts of viral RNAs that contain high-affinity HuR binding sites in their 3′ UTRs effectively serving as a sponge for the HuR protein. Sequestration of HuR by Sindbis virus is associated with destabilization of cellular mRNAs that normally bind HuR and rely on it to regulate their expression. Furthermore, significant changes can be observed in nuclear alternative polyadenylation and splicing events on cellular pre-mRNAs as a result of sequestration of HuR protein by the 3′ UTR of transcripts of this cytoplasmic RNA virus. These studies suggest a molecular mechanism of virus-host interaction that probably has a significant impact on virus replication, cytopathology, and pathogenesis.

  4. UVB-dependent changes in the expression of fast-responding early genes is modulated by huCOP1 in keratinocytes.

    Science.gov (United States)

    Fazekas, B; Polyánka, H; Bebes, A; Tax, G; Szabó, K; Farkas, K; Kinyó, A; Nagy, F; Kemény, L; Széll, M; Ádám, É

    2014-11-01

    Ultraviolet (UV) B is the most prominent physical carcinogen in the environment leading to the development of various skin cancers. We have previously demonstrated that the human ortholog of the Arabidopsis thaliana constitutive photomorphogenesis 1 (COP1) protein, huCOP1, is expressed in keratinocytes in a UVB-regulated manner and is a negative regulator of p53 as a posttranslational modifier. However, it was not known whether huCOP1 plays a role in mediating the UVB-induced early transcriptional responses of human keratinocytes. In this study, we report that stable siRNA-mediated silencing of huCOP1 affects the UVB response of several genes within 2 h of irradiation, indicating that altered huCOP1 expression sensitizes the cells toward UVB. Pathway analysis identified a molecular network in which 13 of the 30 examined UVB-regulated genes were organized around three central proteins. Since the expression of the investigated genes was upregulated by UVB in the siCOP1 cell line, we hypothesize that huCOP1 is a repressor of the identified pathway. Several members of the network have been implicated previously in the pathogenesis of non-melanoma skin cancers; therefore, clarifying the role of huCOP1 in these skin diseases may have clinical relevance in the future.

  5. Effect of HU308 on microglia induced by LPS in secretion of NO and IL-6%HU308对脂多糖诱导小胶质细胞分泌NO及IL-6的影响

    Institute of Scientific and Technical Information of China (English)

    赵建云; 邓展进; 刘瑞珍

    2014-01-01

    Objective To study the effect in secretion of NO and IL-6 of cannabinoid CB2 receptor agonist (HU308) on microglia induced by lipopolysaccharide (LPS). Methods vitro culture BV-2 microglia were divided into control group, LPS stimulation group and intervention group (LPS + HU308). The morphological changes in microglia were observed by microscope, the proliferation in microglia was detected by CCK-8, the NO contents were detected by Griess reaction, the IL-6 contents were detected by ELISA. Results A large number of microglia in LPS stimulation group showed auxetic body, stubby or disappeared pseudopodium and necrotic cells, cell proliferation was poor, the expression level of NO and IL-6 increased significantly. After the intervention of CB2 receptor agonist (HU308), most of microglia showed slightly large body, obvious pseudopodia and light damage, cell proliferation was better, the expression of inflammatory factor decreased significantly. Conclusion Activating CB2 receptors expressing in microglia can alleviate the excessive activation of microglia caused by LPS, inhibit the secretion of N0 and IL-6, so as to achieve the protective effect of central nervous system inflammatory injury.%目的:研究大麻素CB2受体激动剂HU308对脂多糖(LPS)诱导小胶质细胞激活后NO及IL-6分泌的影响。方法体外培养小鼠小胶质细胞株(BV-2细胞),分为对照组、LPS刺激组及干预组(LPS+HU308)。通过显微镜观察各组小胶质细胞的形态学变化,CCK-8法检测各组小胶质细胞的增殖情况,Griess法检测各组NO含量,ELISA法检测各组IL-6水平。结果LPS刺激组的小胶质细胞中出现大量胞体增大,伪足粗短或消失的细胞和一些坏死细胞,细胞增殖较差,NO及IL-6表达水平显著增高。经大麻素CB2受体激动剂HU308干预后,大部分细胞胞体稍大,伪足尚明显,细胞破坏程度轻,增殖较好,炎性因子表达均明显下降。结论激动小

  6. Regional asymmetry of metabolic and antioxidant profile in the sciaenid fish shi drum (Umbrina cirrosa white muscle. Response to starvation and refeeding

    Directory of Open Access Journals (Sweden)

    M. Carmen Hidalgo

    2017-04-01

    Full Text Available The objective of the present study is to characterize the metabolic and antioxidant profile of white muscle of shi drum in two sites of the body, anterior dorsal (AM and posterior dorsal (PM portions. In addition, it will be analyzed the possible effect of starvation and a subsequent refeeding, with two different protocols, pair feeding and ad libitum. Activities of key enzymes of intermediary metabolism and of antioxidant enzymes, as well as lipid peroxidation, as an index of oxidative stress, were evaluated. The results indicate the existence of a regional asymmetry of the metabolic capacities of the white muscle of shi drum, which is likely related to the different contribution to swimming of the body regions examined. Starvation induces a metabolic depression that is more marked in those activities that support burst swimming in PM, while those activities supporting maintenance requirements are conserved. The greatest energy demands during starvation appear to lie in AM, which showed the highest oxidative metabolism rate. The increased use of fatty acids as energy source for AM leads to oxidative stress. A period of more than four weeks of refeeding for full restoration of metabolic capacities in AM is needed, probably related to the higher muscle mass located in this region. On the contrary, all enzyme activities in PM returned to control levels in both refeeding protocols, but pair feeding seems to be advantageous since compensatory growth has been taking place without signs of oxidative stress. This work was addressed to gain knowledge on the physiology of a promising fish species in aquaculture like shi drum. The results displayed here show how the starving and further re-feeding events could generate oxidative stress situations characterized by high lipid peroxidation levels which may influence negatively on the quality of the edible part of the fish. This study opens an interesting field on this fish species which deserves being

  7. Purification and characterization of a novel chlorpyrifos hydrolase from Cladosporium cladosporioides Hu-01.

    Science.gov (United States)

    Gao, Yan; Chen, Shaohua; Hu, Meiying; Hu, Qiongbo; Luo, Jianjun; Li, Yanan

    2012-01-01

    Chlorpyrifos is of great environmental concern due to its widespread use in the past several decades and its potential toxic effects on human health. Thus, the degradation study of chlorpyrifos has become increasing important in recent years. A fungus capable of using chlorpyrifos as the sole carbon source was isolated from organophosphate-contaminated soil and characterized as Cladosporium cladosporioides Hu-01 (collection number: CCTCC M 20711). A novel chlorpyrifos hydrolase from cell extract was purified 35.6-fold to apparent homogeneity with 38.5% overall recovery by ammoniumsulfate precipitation, gel filtration chromatography and anion-exchange chromatography. It is a monomeric structure with a molecular mass of 38.3 kDa. The pI value was estimated to be 5.2. The optimal pH and temperature of the purified enzyme were 6.5 and 40°C, respectively. No cofactors were required for the chlorpyrifos-hydrolysis activity. The enzyme was strongly inhibited by Hg²⁺, Fe³⁺, DTT, β-mercaptoethanol and SDS, whereas slight inhibitory effects (5-10% inhibition) were observed in the presence of Mn²⁺, Zn²⁺, Cu²⁺, Mg²⁺, and EDTA. The purified enzyme hydrolyzed various organophosphorus insecticides with P-O and P-S bond. Chlorpyrifos was the preferred substrate. The Km and Vmax values of the enzyme for chlorpyrifos were 6.7974 μM and 2.6473 μmol·min⁻¹, respectively. Both NH2-terminal sequencing and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometer (MALDI-TOF-MS) identified an amino acid sequence MEPDGELSALTQGANS, which shared no similarity with any reported organophosphate-hydrolyzing enzymes. These results suggested that the purified enzyme was a novel hydrolase and might conceivably be developed to fulfill the practical requirements to enable its use in situ for detoxification of chlorpyrifos. Finally, this is the first described chlorpyrifos hydrolase from fungus.

  8. Investigation into Emperors and Officials' Citation from Shi in the Western Han Dynasty and the Eastern Han Dynasty%两汉君臣用《诗》考

    Institute of Scientific and Technical Information of China (English)

    郑利锋

    2011-01-01

    Shi has been introduced from Qin Dynasty to the Western Han Dynasty and the Eastern Han Dynasty. In both dynasties, there has been much citation of it in the order and report of the emperors and officials. Thus two schools appeared in the defined period with Xi'an as the center of Western Han Dynasty and Luoyang the center of Eastern Han Dynasty. The two centers have demonstrated different styles of citation both in the amount and the features of application owing to their different historical periods and different development of social poli- tics, economics and ideological trends, which have reflected developmental changes and histori- cal features of Shi. These differences have manifested the development and completion of the combination of classicality and historicity of Shi and have been the perfect integration of the po- litical citation of Shi in the Chun Qiu period and the textual citation in the Warring States Period%《诗》自先秦传人两汉,在两汉历朝君臣的下诏和上奏中都大量引用到《诗》,它们分别以西汉的长安和东汉的洛阳为中心,俨然形成两个用《诗》集团。由于所处的历史时代不同和不同历史时段社会政治、经济与学术思想发展的差异,这两个用《诗》集团在引用《诗》之“风”、“雅”、“颂”的数量和在诏奏中征引时所呈现出的应用特征,又有不同;而在此中则反映出了《诗》学在两汉时期的发展流变和时代特征,显示了《诗》之经典性与历史性融通的进行和完成,是春秋时期政治用《诗》和战国时期文本用《诗》的完美融合和发展。

  9. Two Interviews with Shiing-shen Chern and Related%追忆对陈省身的两次访谈及其他

    Institute of Scientific and Technical Information of China (English)

    张友余

    2011-01-01

    追忆陈省身先生在他所接受的两次访谈中对中国数学界提出的殷切期望,介绍杨武之先生教书育人的事绩和成功经验.%In memory of the centennial of the birth of Shiing-Shen Chern, this paper revisits Chern's views and expectations for Chinese mathematics and mathematicians, and recalls the great teaching career and success stories of Mr. W.Z. Young.

  10. Application of pigskin in Guang - shi sausage%猪皮在广式腊肠中的应用研究

    Institute of Scientific and Technical Information of China (English)

    王军锋; 盛本国

    2012-01-01

    The appropriate addition of pigskin during the processing of traditional Guang - shi sausage was determined through the contrast test. The product adding pigskin dachshund was better than the traditional sausage in sensory and physical and , hemical indicators.%在传统广式腊肠的加工过程中添加适量的猪皮,通过对比试验。添加猪皮的腊肠在感官和理化指标上均好于传统腊肠。

  11. A Bite of Hu Flavour in Diets of Tang Dynasty%唐代舌尖上的胡味

    Institute of Scientific and Technical Information of China (English)

    刘锡涛

    2015-01-01

    Tang Dynasty was an innovative, open and tolerant society with booming economy and constant re-newals, which was characterized by strong Hu flavor in diet. There were plenty kinds of Hu diets on staples, vegetables, fruits, wines and flavourings. While pastries were the staple diet, there were such vegetables as spinach, cucumber, eggplant, preserved pickle, lettuce and such fruits as golden peach, grape, date and fla-vourings as sugar, parsley, pepper and such wine as grape wine, long-gao wine. The popularity of Hu diet in Tang Dynasty accelerates the multi-ethnic mélange of dietetic cultures.%唐代是个改革、开放与包容的社会,经济发展,吐旧纳新,人们在饮食上多体现出胡味现象,胡食品种繁多,集中体现在主食、蔬菜、水果、酒及调味品上。主食多吃面饼,蔬菜主要有菠菜、黄瓜、茄子、榨菜、莴苣等,水果有金桃、葡萄、波斯枣等,调味品主要有蔗糖、香菜、胡椒等,酒有葡萄酒、龙膏酒等水果酒。唐代胡食的盛行,加快了我国多民族饮食文化的融合发展。

  12. Les structures génotypiques multilocus des populations d'huîtres plates en France

    Directory of Open Access Journals (Sweden)

    Françoise BLANC

    1987-09-01

    Full Text Available La structure génétique des populations de l'huître plate Ostrea edulis est décrite sur la base des génotypes multilocus. Associée au traitement des données de type multivarié, cette nouvelle procédure permet d'identifier les sous-ensembles génétiques intrapopulationnels qui peuvent être repérés dans un échantillon composite résultant de transferts comme dans la lagune de Thau.

  13. pp32 (ANP32A expression inhibits pancreatic cancer cell growth and induces gemcitabine resistance by disrupting HuR binding to mRNAs.

    Directory of Open Access Journals (Sweden)

    Timothy K Williams

    Full Text Available The expression of protein phosphatase 32 (PP32, ANP32A is low in poorly differentiated pancreatic cancers and is linked to the levels of HuR (ELAV1, a predictive marker for gemcitabine response. In pancreatic cancer cells, exogenous overexpression of pp32 inhibited cell growth, supporting its long-recognized role as a tumor suppressor in pancreatic cancer. In chemotherapeutic sensitivity screening assays, cells overexpressing pp32 were selectively resistant to the nucleoside analogs gemcitabine and cytarabine (ARA-C, but were sensitized to 5-fluorouracil; conversely, silencing pp32 in pancreatic cancer cells enhanced gemcitabine sensitivity. The cytoplasmic levels of pp32 increased after cancer cells are treated with certain stressors, including gemcitabine. pp32 overexpression reduced the association of HuR with the mRNA encoding the gemcitabine-metabolizing enzyme deoxycytidine kinase (dCK, causing a significant reduction in dCK protein levels. Similarly, ectopic pp32 expression caused a reduction in HuR binding of mRNAs encoding tumor-promoting proteins (e.g., VEGF and HuR, while silencing pp32 dramatically enhanced the binding of these mRNA targets. Low pp32 nuclear expression correlated with high-grade tumors and the presence of lymph node metastasis, as compared to patients' tumors with high nuclear pp32 expression. Although pp32 expression levels did not enhance the predictive power of cytoplasmic HuR status, nuclear pp32 levels and cytoplasmic HuR levels associated significantly in patient samples. Thus, we provide novel evidence that the tumor suppressor function of pp32 can be attributed to its ability to disrupt HuR binding to target mRNAs encoding key proteins for cancer cell survival and drug efficacy.

  14. Saturated fatty acids induce post-transcriptional regulation of HAMP mRNA via AU-rich element-binding protein, human antigen R (HuR).

    Science.gov (United States)

    Lu, Sizhao; Mott, Justin L; Harrison-Findik, Duygu Dee

    2015-10-02

    Iron is implicated in fatty liver disease pathogenesis. The human hepcidin gene, HAMP, is the master switch of iron metabolism. The aim of this study is to investigate the regulation of HAMP expression by fatty acids in HepG2 cells. For these studies, both saturated fatty acids (palmitic acid (PA) and stearic acid (SA)) and unsaturated fatty acid (oleic acid (OA)) were used. PA and, to a lesser extent, SA, but not OA, up-regulated HAMP mRNA levels, as determined by real-time PCR. To understand whether PA regulates HAMP mRNA at the transcriptional or post-transcriptional level, the transcription inhibitor actinomycin D was employed. PA-mediated induction of HAMP mRNA expression was not blocked by actinomycin D. Furthermore, PA activated HAMP 3'-UTR, but not promoter, activity, as shown by reporter assays. HAMP 3'-UTR harbors a single AU-rich element (ARE). Mutation of this ARE abolished the effect of PA, suggesting the involvement of ARE-binding proteins. The ARE-binding protein human antigen R (HuR) stabilizes mRNA through direct interaction with AREs on 3'-UTR. HuR is regulated by phosphorylation-mediated nucleo-cytoplasmic shuttling. PA activated this process. The binding of HuR to HAMP mRNA was also induced by PA in HepG2 cells. Silencing of HuR by siRNA abolished PA-mediated up-regulation of HAMP mRNA levels. PKC is known to phosphorylate HuR. Staurosporine, a broad-spectrum PKC inhibitor, inhibited both PA-mediated translocation of HuR and induction of HAMP expression. Similarly, rottlerin, a novel class PKC inhibitor, abrogated PA-mediated up-regulation of HAMP expression. In conclusion, lipids mediate post-transcriptional regulation of HAMP throughPKC- and HuR-dependent mechanisms.

  15. HuR and podoplanin expression is associated with a high risk of malignant transformation in patients with oral preneoplastic lesions

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Higashino, Fumihiro; Hida, Kyoko; Totsuka, Yasunori; Shindoh, Masanobu

    2016-01-01

    The risk of malignant transformation in oral preneoplastic lesions (OPLs) is challenging to assess. The objective of the present study was to determine the expression of ELAV like RNA binding protein 1 (HuR) and podoplanin in OPLs, and to evaluate the use of each protein as biomarkers for the risk assessment of malignant transformations. Immunohistochemistry for HuR and podoplanin was performed on the tissues of 51 patients with OPL, including cases of low grade dysplasia (LGD) and high grade dysplasia (HGD). The association between the protein expression patterns and clinicopathological parameters, including oral cancer free survival (OCFS) time, was analyzed during the follow-up period. HuR and podoplanin expression was observed in 28 (55%) and 36 (71%) of 51 patients, respectively. Kaplan-Meier analysis showed that the expression of HuR and podoplanin was associated with the risk of progression to oral cancer (P<0.05). Multivariate analysis revealed that HuR and podoplanin expression was associated with a 2.93-fold (95% confidence interval (CI), 0.98–10.34; P=0.055) and 2.06-fold (95% CI, 0.55–8.01; P=0.283) increase in risk of malignant transformation, respectively. The risk of OPL malignant transformation was considerably increased with the coexpression of HuR and podoplanin compared with the histological grading (95% CI, 1.64–23.59; P=0.005). The results of the present study demonstrated that the expression of HuR and podoplanin associates with malignant transformation and suggests that the proteins may be used as biomarkers to identify OPL patients with an increased risk of cancer development. PMID:27899983

  16. GC–MS-Based Metabonomic Profiling Displayed Differing Effects of Borna Disease Virus Natural Strain Hu-H1 and Laboratory Strain V Infection in Rat Cortical Neurons

    Directory of Open Access Journals (Sweden)

    Siwen Liu

    2015-08-01

    Full Text Available Borna disease virus (BDV persists in the central nervous systems of a wide variety of vertebrates and causes behavioral disorders. Previous studies have revealed that metabolic perturbations are associated with BDV infection. However, the pathophysiological effects of different viral strains remain largely unknown. Rat cortical neurons infected with human strain BDV Hu-H1, laboratory BDV Strain V, and non-infected control (CON cells were cultured in vitro. At day 12 post-infection, a gas chromatography coupled with mass spectrometry (GC–MS metabonomic approach was used to differentiate the metabonomic profiles of 35 independent intracellular samples from Hu-H1-infected cells (n = 12, Strain V-infected cells (n = 12, and CON cells (n = 11. Partial least squares discriminant analysis (PLS-DA was performed to demonstrate discrimination between the three groups. Further statistical testing determined which individual metabolites displayed significant differences between groups. PLS-DA demonstrated that the whole metabolic pattern enabled statistical discrimination between groups. We identified 31 differential metabolites in the Hu-H1 and CON groups (21 decreased and 10 increased in Hu-H1 relative to CON, 35 differential metabolites in the Strain V and CON groups (30 decreased and 5 increased in Strain V relative to CON, and 21 differential metabolites in the Hu-H1 and Strain V groups (8 decreased and 13 increased in Hu-H1 relative to Strain V. Comparative metabonomic profiling revealed divergent perturbations in key energy and amino acid metabolites between natural strain Hu-H1 and laboratory Strain V of BDV. The two BDV strains differentially alter metabolic pathways of rat cortical neurons in vitro. Their systematic classification provides a valuable template for improved BDV strain definition in future studies.

  17. Increase of the RNA-binding protein HuD and posttranscriptional up-regulation of the GAP-43 gene during spatial memory

    Science.gov (United States)

    Pascale, Alessia; Gusev, Pavel A.; Amadio, Marialaura; Dottorini, Tania; Govoni, Stefano; Alkon, Daniel L.; Quattrone, Alessandro

    2004-01-01

    Neuronal ELAV-like proteins (HuB, HuC, and HuD) are highly conserved RNA-binding proteins able to selectively associate with the 3′ UTR of a subset of target mRNAs and increase their cytoplasmic stability and rate of translation. We previously demonstrated the involvement of these proteins in learning, reporting that they undergo a sustained up-regulation in the hippocampus of mice trained in a spatial discrimination task. Here, we extend this finding, showing that a similar up-regulation occurs in the hippocampus of rats trained in another spatial learning paradigm, the Morris water maze. HuD, a strictly neuron-specific ELAV-like protein, is shown to increase after learning, with a preferential binding to the cytoskeletal fraction. HuD up-regulation is associated with an enhancement of GAP-43 mRNA and protein levels, with an apparently increased HuD colocalization with the GAP-43 mRNA and an increased association of neuronal ELAV-like proteins with the GAP-43 mRNA. These learning-dependent biochemical events appear to be spatiotemporally controlled, because they do not occur in another brain region involved in learning, the retrosplenial cortex, and at the level of protein expression they show extinction 1 month after training despite memory retention. By contrast, HuD mRNA levels still remain increased after 1 month in the CA1 region. This persistence may have implications for long-term memory recall. PMID:14745023

  18. HuR and podoplanin expression is associated with a high risk of malignant transformation in patients with oral preneoplastic lesions.

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Higashino, Fumihiro; Hida, Kyoko; Totsuka, Yasunori; Shindoh, Masanobu

    2016-11-01

    The risk of malignant transformation in oral preneoplastic lesions (OPLs) is challenging to assess. The objective of the present study was to determine the expression of ELAV like RNA binding protein 1 (HuR) and podoplanin in OPLs, and to evaluate the use of each protein as biomarkers for the risk assessment of malignant transformations. Immunohistochemistry for HuR and podoplanin was performed on the tissues of 51 patients with OPL, including cases of low grade dysplasia (LGD) and high grade dysplasia (HGD). The association between the protein expression patterns and clinicopathological parameters, including oral cancer free survival (OCFS) time, was analyzed during the follow-up period. HuR and podoplanin expression was observed in 28 (55%) and 36 (71%) of 51 patients, respectively. Kaplan-Meier analysis showed that the expression of HuR and podoplanin was associated with the risk of progression to oral cancer (P<0.05). Multivariate analysis revealed that HuR and podoplanin expression was associated with a 2.93-fold (95% confidence interval (CI), 0.98-10.34; P=0.055) and 2.06-fold (95% CI, 0.55-8.01; P=0.283) increase in risk of malignant transformation, respectively. The risk of OPL malignant transformation was considerably increased with the coexpression of HuR and podoplanin compared with the histological grading (95% CI, 1.64-23.59; P=0.005). The results of the present study demonstrated that the expression of HuR and podoplanin associates with malignant transformation and suggests that the proteins may be used as biomarkers to identify OPL patients with an increased risk of cancer development.

  19. A case–control study of risk factors for HIV-negative children with cryptococcal meningitis in Shi Jiazhuang, China

    Directory of Open Access Journals (Sweden)

    Guo Jianhua

    2012-12-01

    Full Text Available Abstract Background Although cryptococcal meningitis (CM is an emerging disease worldwide, there have been few studies of the characteristics and risk factors of CM in children. Methods We used data collected from May 2007 through April 2012 in the Acute Meningitis-Encephalitis Syndrome Surveillance project in Shi Jiazhuang, China to describe the epidemiologic, clinical, and laboratory findings in children with CM. Furthermore, a matched case–control study was used to determine risk factors of CM. Results Overall 23 HIV-negative children with CM (median age: 10.91 years; range: 5 months-17 years were enrolled in our study. The average annual incidence of CM was 0.43/100,000 with a fatality rate of 1.7%. Most patients were males (60.87% and rural children (73.91%. Common clinical symptoms included increased intracranial pressure, such as headaches (78.3%, nausea (60.9%, altered mental status (56.5%, vomiting (52.2%, and seizures (43.5%, and frequent laboratory findings consisted of blood leukocytosis (87.0%, decreased CSF glucose (87.0%, pleocytosis (82.6%, increased intracranial pressure (73.9% and elevated CSF proteins (65.2%. Epidemiologic, clinical, and laboratory findings were similar between patients with and without underlying diseases. Multivariate logistic regression analysis showed that children who had contact with birds/bird droppings or saprophytes were more likely to be infected than those without such contact (odds ratio(OR =11.82; 95% confidence interval (CI, 2.21-62.24; P = 0.004. Patients with an interval of ≥20 days from onset to admission were at high risk for CM (OR= 5.31; 95%CI, 1.58-17.89; P = 0.007. Conclusions Our findings show that CM is an uncommon disease with a high mortality rate in children. Although additional studies are needed to find effective prevention and treatments for CM, clinicians should consider CM as a potential cause for pediatric meningitis in children, particularly boys from

  20. Influence of SHI upon nanohole free volume and micro scale level surface modifications of polyethyleneterephthalate polymer films

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Rajesh, E-mail: rajeshkumaripu@gmail.com; Singh, Paramjit

    2015-05-15

    Highlights: • Ion irradiation of PET polymer (used for gas separation applications) for structural, optical, chemical, surface and free volume studies. • The amorphization and decrease of the band gap energy after ions irradiation. • The value of R increased after ion irradiation due to the expansion of the polymeric chains and increase of the size of cavities. • Increase in the surface roughness creating the site of track formation. - Abstract: Topographic micro scale and in-depth nano scale level modifications of polymeric materials play an important role in engineering their physical and chemical properties. Polyethylene terephthalate (PET) is an important class of semi-crystalline polymers used for gas separation properties. The gas diffusion and permeability parameters are directly related to the free volume fractions and the hole distributions. The controlled and precise ion beam irradiation can be used to induce surface and in-depth modifications in the properties of the polymers which help in modifying free volume holes and their distributions. In the present study, the investigation of free volume (nano scale level) and surface (micro scale level) properties of PET polymeric thin films after SHI treatment were employed by means of positron annihilation lifetime spectroscopy (PALS) and atomic force microscopy (AFM), respectively. The PET thin films were irradiated by 50 MeV lithium ions as a function of ion fluence. The value of hole radius (R) and intensity (I{sub 3}) of o-Ps were observed to be increased after ion beam treatment. The further analyses were employed to calculate the free volume and fractional free volume of holes from the obtained values of R and I{sub 3}. The AFM studies reveal the surface modifications of the irradiated polymer films. The structural, optical and chemical properties were investigated by X-ray diffraction (XRD), UV–visible (UV–vis) and Fourier transform infrared (FTIR) spectrophotometry. Different parameters

  1. Neutron activation analysis of sources of raw material of Emperor Qin Shi Huang's Terracotta Warriors and Horses

    Science.gov (United States)

    Gao, Zhengyao; Zhao, Weijuan; Li, Guoxia; Xie, Jianzhong; Han, Guohe; Feng, Songlin; Fan, Dongyu; Zhang, Ying; Chai, Zhifang; Li, Rongwu; Zhang, Zhongli; Zhu, Junxiao

    2003-02-01

    There have been selected 83 samples of terracotta warriors and horses of Emperor Qin Shi Huang’s Mausoleum, 20 samples of clays taken from around Qin’s Mausoleum and 2 samples of Yaozhou porcelain bodies. All these samples have been measured by instrument neutron activation analysis (INAA) and as many as 32 kinds of element contents of each sample are measured. The following conclusion has been reached when fuzzy cluster analysis is conducted to element contents of all these samples: (i) The samples are roughly classified into five categories: namely, samples from pits No. 1 and No. 2; samples from pit No. 3; loam layers; the mixture of loam and loess; and Yaozhou porcelain bodies. (ii) The terracotta warriors and horses in pits No. 1, No. 2 and No. 3 are relatively independent from one another. The clays from which they were made are not entirely identical. We have found that samples in pit No. 3 are very closely related and their clay sources are comparatively concentrated. Samples in pits No. 1 and No. 2 are less related and their clay sources are comparatively scattered. (iii) The clays from which the terracotta warriors and horses were made are closely related to the loam layer near Qin’s Mausoleum, particularly to the loam layer of Zaoyuan village and Gaoxing village, but they are not so related to loess layers there, nor to the loam layers of Anhoubao, even less related to Yaozhou porcelain bodies. A rational deduction thus drawn is that the raw material of clays from which the terracotta warriors and horses were made might probably be taken from loam layers around Zaoyuan and Gaoxing, or loam layers near Qin’s Mausoleum whose properties are identical with those of loam layers of Zaoyuan and Gaoxing, rather than loess layers around the above places. Since the raw material of the terracotta warriors and horses was taken from loam near Qin’s Mausoleum, it could be deducted that the kiln sites might be located in around Qin’s Mausoleum.

  2. Construction of Eukaryotic Expression Vector Containing HuCTLA4Ig and its Expression in Mammalian Cells%HuCTLA4Ig真核表达载体的构建及其表达

    Institute of Scientific and Technical Information of China (English)

    祁小平; 钱军华; 谢晋; 马宝骊

    2000-01-01

    用RT-PCR方法从人外周血单个核细胞总RNA中分别扩增出CTLA4的胞膜外区基因和Igγ1恒定区基因,将它们拼接后插入质粒pcDNA3.将重组的HuCTLA4Ig/pcDNA质粒转染细胞,经筛选得到了高效、稳定表达HuCTLA4Ig的克隆.培养上清经ProteinA亲和层析柱纯化后,所得蛋白在非还原条件下SDS-PAGE呈分子量为11kD的一条带;FACS检测显示该纯化蛋白与B7分子有良好的结合能力;在体外能抑制MLR的增殖反应.

  3. Impact on Shy Individuals' Inclination of Social Behaviors by Social Cues%社交线索对害羞者社交行为倾向的影响

    Institute of Scientific and Technical Information of China (English)

    金一波; 王文娟; 张锋; 李娇

    2012-01-01

    With the development of the society, the meaning of shyness has gradually changed in China. The negative impact of shy- ness on the quality of people's life becomes more and more obvious. Shy people experience more disadvantages in the fierce social com- petition. However, compared with the numerous researches on anxiety and depression in the past few decades, researches on shyness in the field of psychology appear few and far between. As a social cognitive phenomenon, shyness is closely related to social contexts. Pre- vious researches based on clinical observation and questionnaire assessment were limited to the description of the static behavior charac- teristics of shy individuals ( e. g. the social withdrawal behaviors in the social intercourse situation, hypersensitivity of the threat infor- mation) , while they ignored the dynamic process of how these characteristics affect the withdrawal behavior of shy individuals. There- fore, this study aimed to explore the impact of different social cues upon shy individuals' inclination of social behaviors from a socio-cog- nitive perspective. A cue-exposure paradigm was used in this study, and the stimuli were real facial expressions including angry, neutral and happy faces. A 3 (angry/neutral/happy) ×2 (high shy/low shy) × 2 (before elicit/after elicit) mixed design was adopted to compare the dif- ferent levels of the subjects' inclination to participate in speech. A sample of 89 high-shy individuals and 84 low-shy individuals partici- pated in the experimental as subjects. A VAS (visual analogue scale) was used to measure the subjects' inclination. SPSS was used to analyze the data. It was shown by ANOVA that the main effect in facial expressions properties ( F = 5. 236, p = . 006 ) was found, that the main effect in groups (F= 16. 846, p =. 000) was found, that the main effect in assessing time (F =39. 520, p =. 000) and the interaction effect ( F = 3. 993, p =. 020) among the three variable were found. Further

  4. The Research of Su Shi and Li Zhi ’s Relationship%苏轼与李廌交游考

    Institute of Scientific and Technical Information of China (English)

    彭文良

    2015-01-01

    “苏门六君子”之一李廌,乃苏轼同年李惇之子,与苏轼长子苏迈同岁出生,六岁而孤。苏轼对李廌,呵护备至,期待极高,一直以道义名节相许。李廌虽才华横溢,然仕途一直不顺,苏轼不遗余力地加以提携,但从未丧失过基本的原则和底线。%Li Zhi, the son of Li Dun, was a member of a famous poet group———“Six Scholars of Su’s Family”. He was the same age as Su Shi’s eldest son, Su Mai. After Li Zhi’s parents died when he was only six years old, Su Shi took great care of him and had high expectations for his great fame and moral respon-sibility. Although Li Zhi was very talented, he had been suffering an uneven career for a long time. In the lowest point of Li Zhi’s career, Su Shi tried his best to help him but never lost his essential principles and basic bottom line.

  5. A Tentative Study on Yang Shi's Ideas about the Cultivation of the Mind%杨时心性修养思想探微

    Institute of Scientific and Technical Information of China (English)

    兰宗荣

    2014-01-01

    Yang Shi thought knowing benevolence, mind and internal tendencies as well as destiny were the bases for the cultivation of the mind, faithfulness and integrity were the keys to the cultivation of the mind, embodying the golden mean of the Confucian school through all the showed joys and sorrows of life and potential consciousness was the important way of the cultivation of the mind, and conforming to fate and consummating oneself and the nature were the goal of the cultivation of the mind. Yang Shi's mind philosophy was an important link in the inheritance and transition of Neo-Confucianism in the Song and Ming Dynasty.%杨时认为知仁、知心、知性知天是心性修养的基础;意诚心正是心性修养的关键;“未发体中,已发得和”是心性修养的重要法门;顺应天命,以成己成物是心性修养的目标。杨时的心性哲学是宋明理学传承与转化中不可或缺的一环。

  6. 西安市北周史君石椁墓%Shi Sarcophagused Tomb of the Northern Zhou Period in Xi'an

    Institute of Scientific and Technical Information of China (English)

    西安市文物保护考古所

    2004-01-01

    In 2003, the Xi'an Municipal Institute of Ancient Monument Preservation and Archaeology excavated the Shi tomb of the Northern Zhou period east of the Jingshang Village of Daminggong Township in Weiyang District, Xi'an City. They discovered stone sealing slabs, a sarcophagus and beds, as well as gold finger-rings, coins and ornaments, all valuable cultural relics. The tomb is an earthen cave, and consists of a long ramp, small yards, tunnels, a corridor and a chamber. The tombgate, lintel, ramp and chamber are decorated with murals. In the tomb is a couple's joint burial. The male tomb-owner, Shi by name, was the Sabao, or administrator of Zoroastrianism, in the Liangzhou Prefecture of the Northern Zhou period. The color-painted and gold-foiled carvings in relief on the sarcophagus, beds and sealing stones reflect both the Zoroastrianist culture and influence from the Han culture. They provide invaluable textual and pictorial data for studying the Silk Road and Sino-western cultural relations, and have very great significance to researching into the history of Sino-western cultural relations and that of Chinese art.

  7. 贾雨村讹夺石扇案辨正%Explore the Truth of JIA Yu-cun Grabbing SHI Dai-zi's Fans by Blackmail

    Institute of Scientific and Technical Information of China (English)

    房义斌

    2011-01-01

    学界历来认为讹夺石扇为贾雨村欺压良善的铁证,不假辨别地信以为真,对其人品大加挞伐,实属误会。讹夺石扇是平儿、贾琏合谋的谎言,是对纳妾鸳鸯事件的影射,并非贾琏挨打的真相。贾琏挨打的导火索是鸳鸯事件;远因是大不孝——无子。%Academia has regarded the case of JIA Yu-cun grabbing SHI Dai-zi's fans by blackmail as an indisputable fact of his bully oppression of good people without identification. Then lots of critics of JIA Yu-cun's moral character leaded to injustice, which is a misunderstanding. The truth of JIA Yu-cun grabbing SHI Dai-zi's fans by blackmail was a mendacity collaborated by Ping Er and JIA Lian, wich was a shadow Of Yuan Yang event actually, not the truth of JLA Lian beaten. The fuse of JIA Lian beaten was Yuan Yang event, and the underlying cause was that JIA Lian had no son.

  8. The Features and Significance of Learning Su Shi in Literature of Jin Dynasty%金代文学学苏的特点与意义

    Institute of Scientific and Technical Information of China (English)

    任建国; 狄宝心

    2013-01-01

    金代文学在继承北宋文学方面与南宋同,但取向不同。苏学北盛是以女真统治文化为主导的北方文化与中原文化双向交流的产物,优势互补对金代文学雄健特色的形成作用重大。苏学北盛对于了解金人在民族文化融合过程中的文化心理也有重要意义。%Like South Song Dynasty literature of Jin Dynasty also succeeded literature of North Song,besides the orientations. In the north part of China where the Jin Dynasty ruled,the prosperity of Su Shi study is the consequence of cultural communication between North Culture and Middle Culture of China,the complement contributed to the development of literature of Jin dynasty. Study on learning Su Shi will be significant for study on the cultural psychology of ethnic group in the process of national integration.

  9. SHI induced effects on the electrical and optical properties of HfO{sub 2} thin films deposited by RF sputtering

    Energy Technology Data Exchange (ETDEWEB)

    Manikanthababu, N.; Dhanunjaya, M.; Nageswara Rao, S.V.S.; Pathak, A.P., E-mail: appsp@uohyd.ernet.in

    2016-07-15

    The continuous downscaling of Metal Oxide Semiconductor (MOS) devices has reached a limit with SiO{sub 2} as a gate dielectric material. Introducing high-k dielectric materials as a replacement for the conservative SiO{sub 2} is the only alternative to reduce the leakage current. HfO{sub 2} is a reliable and an impending material for the wide usage as a gate dielectric in semiconductor industry. HfO{sub 2} thin films were synthesized by RF sputtering technique. Here, we present a study of Swift Heavy Ion (SHI) irradiation with100 MeV Ag ions for studying the optical properties as well as 80 MeV Ni ions for studying the electrical properties of HfO{sub 2}/Si thin films. Rutherford Backscattering Spectrometry (RBS), Field Emission Scanning Electron Microscope (FESEM), energy-dispersive X-ray spectroscopy (EDS), profilometer and I–V (leakage current) measurements have been employed to study the SHI induced effects on both the structural, electrical and optical properties.

  10. HuR cytoplasmic expression is associated with increased cyclin A expression and poor outcome with upper urinary tract urothelial carcinoma

    Directory of Open Access Journals (Sweden)

    Liang Peir-In

    2012-12-01

    Full Text Available Abstract Background HuR is an RNA-binding protein that post-transcriptionally modulates the expressions of various target genes implicated in carcinogenesis, such as CCNA2 encoding cyclin A. No prior study attempted to evaluate the significance of HuR expression in a large cohort with upper urinary tract urothelial carcinomas (UTUCs. Methods In total, 340 cases of primary localized UTUC without previous or concordant bladder carcinoma were selected. All of these patients received ureterectomy or radical nephroureterectomy with curative intents. Pathological slides were reviewed, and clinical findings were collected. Immunostaining for HuR and cyclin A was performed and evaluated by using H-score. The results of cytoplasmic HuR and nuclear cyclin A expressions were correlated with disease-specific survival (DSS, metastasis-free survival (MeFS, urinary bladder recurrence-free survival (UBRFS, and various clinicopathological factors. Results HuR cytoplasmic expression was significantly related to the pT status, lymph node metastasis, a higher histological grade, the pattern of invasion, vascular and perineurial invasion, and cyclin A expression (p = 0.005. Importantly, HuR cytoplasmic expression was strongly associated with a worse DSS (p p p = 0.0370 in the univariate analysis, and the first two results remained independently predictive of adverse outcomes (p = 0.038, relative risk [RR] = 1.996 for DSS; p = 0.027, RR = 1.880 for MeFS. Cyclin A nuclear expression was associated with a poor DSS (p = 0.0035 and MeFS (p = 0.0015 in the univariate analysis but was not prognosticatory in the multivariate analyses. High-risk patients (pT3 or pT4 with/without nodal metastasis with high HuR cytoplasmic expression had better DSS if adjuvant chemotherapy was performed (p = 0.015. Conclusions HuR cytoplasmic expression was correlated with adverse phenotypes and cyclin A overexpression and also independently predictive

  11. A new human cholangiocellular carcinoma cell line (HuCC-T1) producing carbohydrate antigen 19/9 in serum-free medium.

    Science.gov (United States)

    Miyagiwa, M; Ichida, T; Tokiwa, T; Sato, J; Sasaki, H

    1989-06-01

    A human cholangiocellular carcinoma cell line, HuCC-T1, was established in vitro from the malignant cells of ascites of a 56-yr-old patient. Histologic findings of the primary liver tumor revealed a moderately differentiated adenocarcinoma. Tumor cells from the ascites have been cultured with RPMI 1640 medium containing 0.2% lactalbumin hydrolysate and the cultured cells grew as monolayers with a population doubling time of 74 h during exponential growth at Passage 25. They had an epithelial-like morphology and were positive for mucine staining. Ultrastructural studies revealed the presence of microvilli on the cell surface and poorly developed organelles in the cytoplasm. The HuCC-T1 cell was tumorigenic in nude mice. The number of chromosomes in HuCC-T1 ranged from 61 to 80. These human cholangiocellular carcinoma cells in serum-free medium secreted several tumor markers, including carbohydrate antigen 19/9, carbohydrate antigen 125, carcinoembryonic antigen, and tissue polypeptide antigen. The carbohydrate antigen 19/9 secretion level of HuCC-T1 cells cultured in RPMI 1640 medium with 1% fetal bovine serum was sixfold higher than that with 0.2% lactalbumin hydrolysate. These findings suggest that HuCC-T1 will provide useful information to clarify the mechanism of tumor marker secretion and tumor cell growth in the human cholangiocellular carcinoma.

  12. Comparison between two treatment protocols with recombinant Human Erythropoietin (rHuEpo in the treatment of late anemia in neonates with Rh-Isoimmunization

    Directory of Open Access Journals (Sweden)

    A.A. Zuppa

    2012-08-01

    Full Text Available Objectve. The Rh-hemolytic disease can lead to a late anemia by hemolytic and hyporigenerative mechanism. We compared the effectiveness of rHuEPO in two care protocols that differ for doses of rHuEPO administrated and for timing of administration. Methods. A cohort of 14 neonates was investigated. The neonates were treated with two different protocols. Protocol A: a dose of 200 U/kg/day of rHuEpo administered subcutaneously starting from the end of the second week of life; Protocol B: a dose of 400 U/kg/day of rHuEpo administered subcutaneously starting from the end of the first week of life. Results. The hematocrit values in the protocol A group decreased during treatment (32,5% vs 25,2%, whereas the hematocrit value in protocol B group remained almost stable (38,7% vs 42,8%. The mean numbers of platelets remained stable in both groups while neutrophils increased in protocol A group and decreased in protocol B (p<0,05. Reticulocyte count increased during treatment in both groups, although only in protocol B group it was statistically significative (p<0,05. Conclusions. Our results suggest a similar efficacy between the two treatment protocols. Increasing doses of rHuEPO do not seem enhancing their effectiveness and the incidence of side effects.

  13. Pharmacological Progress of HuZhang%中药虎杖药理作用研究进展

    Institute of Scientific and Technical Information of China (English)

    黄海量

    2012-01-01

    总结近年来关于中药虎杖中有效成分治疗心血管系统、消化系统、呼吸系统、内分泌系统、生殖系统、免疫系统等疾病的文献报道,发现虎杖含有的丰富活性成分具有多项药理作用,临床应用日益广泛,但其毒理研究和有效成分提取方法的报道鲜见.%Literature and reports on effective constituents of HuZhang (Polygonum cuspidatum) in treating the diseases of cardiovascular system, digestive system, respiratory system, endocrine system, reproductive system, immune system and so on were concluded in recent years and the result of study showed that rich active constituents in HuZhang had pharmacological functions and were widely used day by day, but toxicological studies and extraction of effective constituent were rarely reported.

  14. Optimization and risk analyses for rule curves of reservoir operation: application to Tien-Hua-Hu Reservoir in Taiwan.

    Science.gov (United States)

    Kuo, J T; Hsu, N S; Chiu, S K

    2006-01-01

    Tien-Hua-Hu Reservoir is currently under planning by the Water Resources Agency, Taiwan to meet the increasing water demands of central Taiwan arising from rapid growth of domestic water supply, and high-tech industrial parks. This study develops a simulation model for the ten-day period reservoir operation to calculate the ten-day water shortage index under varying rule curves. A genetic algorithm is coupled to the simulation model to find the optimal rule curves using the minimum ten-day water shortage index as an objective function. This study generates many sets of synthetic streamflows for risk, reliability, resiliency, and vulnerability analyses of reservoir operation. ARMA and disaggregation models are developed and applied to the synthetic streamflow generation. The optimal rule curves obtained from this study perform better in the ten-day shortage index when compared to the originally designed rule curves from a previous study. The optimal rule curves are also superior to the originally designed rule curves in terms of vulnerability. However, in terms of reliability and resiliency, the optimal rule curves are inferior to the those originally designed. Results from this study have provided in general a set of improved rule curves for operation of the Tien-Hua-Hu Reservoir. Furthermore, results from reliability, resiliency and vulnerability analyses offer much useful information for decision making in reservoir operation.

  15. r-huEPO在胃肠道恶性肿瘤患者围手术期应用

    Institute of Scientific and Technical Information of China (English)

    缪传文; 李继坤; 温玉刚; 周立生; 李旭

    2006-01-01

    胃肠道恶性肿瘤往往伴有程度不等的贫血,以往临床上除了输血疗效确切外,只有使用各种生血制剂,主要是铁剂,往往起效缓慢,难以令人满意。而输血由于血源紧张,还有各种输血的并发症,及传播各种严重传染病性疾病的危险,使用受到限制。重组人促红细胞生成素(recombinant erythropoietin)的围手术期红细胞动员也越来越受到临床重视,有研究表现大剂量应用r-huEPO有明显的红细胞动员作用。2003年-2005年,我们应用r-huEPO治疗胃肠道恶性肿瘤伴贫血的手术患者276例,取得了较好的疗效。

  16. Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell

    Directory of Open Access Journals (Sweden)

    Aris Haryanto

    2015-10-01

    Full Text Available The application of fluorescent proteins as expression markers and protein fusion partners has provedimmensely valuable for resolving the organization of biological events in living cells. EGFP and DsRed2 arecommonly fluorescent marker protein which is used for biotechnology and cell biology research. The presentstudy was designed to identify the expression vector that suitable to ligate with DNA encoding HBV coreprotein for intracellular localization study in hepatocyte cell, which were expressed as fusion proteins. We alsocompared and quantified the expressed fluorescent protein which predominantly localized in the cellcompartment. The results indicated that DsRed2 shown as less than ideal for intracellular localization study ofthan EGFP, because of its tetrameric structure of the fluorescent protein and when fused to a protein of interest,the fusion protein often forms aggregates in the living cells. In contrast, EGFP fluorescent protein shown a muchhigher proportion of cytoplasmic localization, thus being more suitable for analysis of intracellular localizationthan DsRed2 fluorescent protein. EGFP fluorescent protein is also capable to produce a strong green fluorescencewhen excited by blue light, without any exogenously added substrate or cofactor, events inside living cell canthus be visualized in a non-invasive way. Based on our present quantitative data and some reasons above shownthat EGFP is more suitable than DsRed2 as a fluorescent marker protein for intracellular localization study intoHuH-7 cell.Keywords: EGFP, DsRed2 fluorescent protein , HuH-7 cell, HBV, intracellular localization

  17. Mouse monocyte/macrophage cell line J774A.1 migration induced by the activation of CB1 depends on HuR%激活大麻素受体1诱导的单核巨噬细胞J774A.1的迁移依赖RNA结合蛋白HuR

    Institute of Scientific and Technical Information of China (English)

    赵中新; 常娜; 盖菁菁; 田蕾; 李丽英

    2016-01-01

    目的 研究大麻素受体1(cannabinoid receptor 1,CB1)在单核巨噬细胞迁移中的重要作用以及RNA结合蛋白人抗原R (human antigen R,HuR)参与其中的可能机制.方法 选用单核巨噬细胞系J774A.1,应用琼脂糖凝胶电泳和免疫荧光染色技术鉴定J774A.1中CB1以及HuR的表达;ACEA和AM281分别为CB1的药理学激动剂和拮抗剂,应用Boyden chamber法检测ACEA和AM281对J774A.1迁移活性的影响.HuR的基因干扰用于确定激活CB1诱导的J774A.1迁移功能是否依赖HuR;胞质蛋白的分离用于探究激活CB1是否能引起J774A.1胞质中HuR的富集;RT-qPCR和Western blotting法检测CB1和HuR mRNA和蛋白质的变化情况.结果 该研究证明J774A.1在基因和蛋白质水平上均表达CB1和HuR;激活CB1能够促进J774A.1的迁移(P<0.01)并且能够被其药理学拮抗剂AM281所抑制;激活CB1诱导的J774A.1的迁移依赖HuR;激活CB1促进了J774A.1胞质中HuR的富集进一步影响了CB1的表达,由此HuR参与了激活CB1诱导的J774A.1的迁移.结论 激活CB1能够诱导单核巨噬细胞系J774A.1的迁移,且此过程依赖RNA结合蛋白HuR.

  18. Human-derived IgG level as an indicator for EBV-associated lymphoma model in Hu-PBL/SCID chimeras.

    Science.gov (United States)

    Tang, Yunlian; He, Rongfang; Zhang, Yang; Liu, Fang; Cheng, Ailan; Wu, Yimou; Gan, Runliang

    2011-05-09

    Epstein-Barr virus (EBV) has a close association with various types of human lymphomas. Animal models are essential to elucidate the pathogenesis of human EBV-associated lymphomas. The aim of the present study is to evaluate the association between human IgG concentration and EBV-associated lymphoma development in huPBL/SCID mice. Human peripheral blood lymphocytes (hu-PBL) from EBV-seropositive donors were inoculated intraperitoneally into SCID mouse. Immunohistochemical staining was used to examine differentiated antigens of tumor cells. EBV infection of the induced tumors was detected by in situ hybridization. IgG concentrations in the serums of 12 SCID mice were measured by unidirectional immunodiffusion assay. 21 out of 29 mice developed tumors in their body. Immunohistochemical staining showed that all induced tumors were LCA (leukocyte common antigen) positive, B-cell markers (CD20, CD79a) positive, and T-cell markers (both CD3 and CD45RO) negative. The tumors can be diagnosed as human B-cell lymphomas by these morphological and immunohistochemical features. In situ hybridization exhibited resultant tumor cells had EBV encoded small RNA-1 (EBER-1). Human-derived IgG could be found in the serum from SCID mice on the 15th day following hu-PBL transplantation, and IgG levels increased with the tumor development in 6 hu-PBL/SCID chimeras. Intraperitoneal transfer of hu-PBLs from EBV+ donors to SCID mice leads to high human IgG levels in mouse serum and B cell lymphomas. Our findings suggest that increasing levels of human-derived IgG in peripheral blood from hu-PBL/SCID mice could be used to monitor EBV-related human B-cell lymphoma development in experimental animals.

  19. Development and Characterization of a Humanized Anti-HER2 Antibody HuA21 with Potent Anti-Tumor Properties in Breast Cancer Cells.

    Science.gov (United States)

    Li, Ruilin; Hu, Siyi; Chang, Yan; Zhang, Zhihui; Zha, Zhao; Huang, Hui; Shen, Guodong; Liu, Jing; Song, Lihua; Wei, Wei

    2016-04-15

    Human epidermal growth factor receptor 2 (HER2) is one of the most studied tumor-associated antigens for cancer immunotherapy. An engineered anti-HER-2 chimeric A21 antibody (chA21) is a chimeric antibody targeted to subdomain I of the HER2 extracellular domain. Here, we report the anti-tumor activity of the novel engineered monoclonal antibody humanized chA21 (HuA21) that targets HER2 on the basis of chA21, and we describe the underlying mechanisms. Our results reveal that HuA21 markedly inhibits the proliferation and migration of HER2-overexpressing breast cancer cells and causes enhanced antibody-dependent cell-mediated cytotoxicity potency against HER2-overexpressing tumor cells. In particular, HuA21, but not trastuzumab (Tra), markedly suppresses growth and enhances the internalization of the antibody in Tra-resistant BT-474 breast cancer cells. These characteristics are highly associated with the intrinsic ability of HuA21 to down-regulate HER2 activation and inhibit the extracellular signal-regulated kinase 1/2 (ERK1/2) and protein kinase B (Akt) signaling pathways. Furthermore, the combination of HuA21 with Tra synergistically enhances the anti-tumor effects in vitro and in vivo and inhibits HER2 activation and the ERK1/2 and Akt signaling pathways. Altogether, our results suggest that HuA21 may represent a unique anti-HER2 antibody with potential as a therapeutic candidate alone or in combination with other anti-HER2 reagents in cancer therapy.

  20. Development and Characterization of a Humanized Anti-HER2 Antibody HuA21 with Potent Anti-Tumor Properties in Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Ruilin Li

    2016-04-01

    Full Text Available Human epidermal growth factor receptor 2 (HER2 is one of the most studied tumor-associated antigens for cancer immunotherapy. An engineered anti-HER-2 chimeric A21 antibody (chA21 is a chimeric antibody targeted to subdomain I of the HER2 extracellular domain. Here, we report the anti-tumor activity of the novel engineered monoclonal antibody humanized chA21 (HuA21 that targets HER2 on the basis of chA21, and we describe the underlying mechanisms. Our results reveal that HuA21 markedly inhibits the proliferation and migration of HER2-overexpressing breast cancer cells and causes enhanced antibody-dependent cell-mediated cytotoxicity potency against HER2-overexpressing tumor cells. In particular, HuA21, but not trastuzumab (Tra, markedly suppresses growth and enhances the internalization of the antibody in Tra-resistant BT-474 breast cancer cells. These characteristics are highly associated with the intrinsic ability of HuA21 to down-regulate HER2 activation and inhibit the extracellular signal-regulated kinase 1/2 (ERK1/2 and protein kinase B (Akt signaling pathways. Furthermore, the combination of HuA21 with Tra synergistically enhances the anti-tumor effects in vitro and in vivo and inhibits HER2 activation and the ERK1/2 and Akt signaling pathways. Altogether, our results suggest that HuA21 may represent a unique anti-HER2 antibody with potential as a therapeutic candidate alone or in combination with other anti-HER2 reagents in cancer therapy.

  1. HIV-1 Pathogenesis and Therapeutic Intervention in the SCID-hu Thy/Liv Mouse: A Model for Primary HIV-1 Infection in the Human Thymus

    OpenAIRE

    Su, Lishan

    1997-01-01

    The SCID-hu Thy/Liv mouse is a model for the analysis of human thymopoiesis. It has been constructed by engrafting fragments of human fetal liver and thymus into the immunodeficient C.B-17 scid/scid (SCID) mouse. The resulting ‘Thy/Liv’ organ promotes long-term differentiation of human T cells. Given the apparently normal physiology of the SCID-hu Thy/Liv organ, it has been used to explore the pathophysiologic mechanisms of HIV-1 infection in vivo, and to test therapeutic modalities such as a...

  2. HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-γc-/- (RAG-hu mouse model

    Directory of Open Access Journals (Sweden)

    Connick Elizabeth

    2006-11-01

    Full Text Available Abstract Background The currently well-established humanized mouse models, namely the hu-PBL-SCID and SCID-hu systems played an important role in HIV pathogenesis studies. However, despite many notable successes, several limitations still exist. They lack multi-lineage human hematopoiesis and a functional human immune system. These models primarily reflect an acute HIV infection with rapid CD4 T cell loss thus limiting pathogenesis studies to a short-term period. The new humanized Rag2-/-γc-/- mouse model (RAG-hu created by intrahepatic injection of CD34 hematopoietic stem cells sustains long-term multi-lineage human hematopoiesis and is capable of mounting immune responses. Thus, this model shows considerable promise to study long-term in vivo HIV infection and pathogenesis. Results Here we demonstrate that RAG-hu mice produce human cell types permissive to HIV-1 infection and that they can be productively infected by HIV-1 ex vivo. To assess the capacity of these mice to sustain long-term infection in vivo, they were infected by either X4-tropic or R5-tropic HIV-1. Viral infection was assessed by PCR, co-culture, and in situ hybridization. Our results show that both X4 and R5 viruses are capable of infecting RAG-hu mice and that viremia lasts for at least 30 weeks. Moreover, HIV-1 infection leads to CD4 T cell depletion in peripheral blood and thymus, thus mimicking key aspects of HIV-1 pathogenesis. Additionally, a chimeric HIV-1 NL4-3 virus expressing a GFP reporter, although capable of causing viremia, failed to show CD4 T cell depletion possibly due to attenuation. Conclusion The humanized RAG-hu mouse model, characterized by its capacity for sustained multi-lineage human hematopoiesis and immune response, can support productive HIV-1 infection. Both T cell and macrophage tropic HIV-1 strains can cause persistent infection of RAG-hu mice resulting in CD4 T cell loss. Prolonged viremia in the context of CD4 T cell depletion seen in this

  3. Analysis of Shi Tuo's View of Modern Love and Marriage%师陀的现代婚恋观探析

    Institute of Scientific and Technical Information of China (English)

    王欣

    2012-01-01

    Shi Tuo is a writer with unique personality and strong human feelings in modern Chinese lite-rature.The relation between two sexes is an entry point for his thinking about life and social problems.In his literary works,he is involved in the process of modernization by the construction and expression of modern love and marriage.Shi Tuo's views of modern love and marriage are as follows: the ideal family life should be the peaceful and quiet rural life based on mutual love of two sexes;the solid economic condition is the foundation of a marriage,however,excessive pursuit of money may destroy love;an ideal woman should be one both with traditional virtues and subject consciousness.Shi Tuo strives to seek reconciliation of two sexes in a healthy marital life,which is the premise of people's happiness.%师陀是中国现代文坛上具有独特创作个性和浓厚人文情怀的作家。两性关系是他思考人生和社会问题的一个切入口。师陀作品中的现代婚恋观表现为:理想的家庭生活,是以男女互爱为基础的、和平宁静的田园诗般的生活;稳固的经济基础才能强大婚姻自身,但过度地追求金钱,也会毁灭爱情;理想的女性,既有传统美德,又具备主体意识。师陀设法在一种健康的婚恋关系中寻求男人和女人之间的和解,因为它是整个人类获得幸福与和谐的先决条件。

  4. Test of Shi et al. Method to Infer the Magnetic Reconnection Geometry from Spacecraft Data: MHD Simulation with Guide Field and Antiparallel Kinetic Simulation

    Science.gov (United States)

    Denton, R.; Sonnerup, B. U. O.; Swisdak, M.; Birn, J.; Drake, J. F.; Heese, M.

    2012-01-01

    When analyzing data from an array of spacecraft (such as Cluster or MMS) crossing a site of magnetic reconnection, it is desirable to be able to accurately determine the orientation of the reconnection site. If the reconnection is quasi-two dimensional, there are three key directions, the direction of maximum inhomogeneity (the direction across the reconnection site), the direction of the reconnecting component of the magnetic field, and the direction of rough invariance (the "out of plane" direction). Using simulated spacecraft observations of magnetic reconnection in the geomagnetic tail, we extend our previous tests of the direction-finding method developed by Shi et al. (2005) and the method to determine the structure velocity relative to the spacecraft Vstr. These methods require data from four proximate spacecraft. We add artificial noise and calibration errors to the simulation fields, and then use the perturbed gradient of the magnetic field B and perturbed time derivative dB/dt, as described by Denton et al. (2010). Three new simulations are examined: a weakly three-dimensional, i.e., quasi-two-dimensional, MHD simulation without a guide field, a quasi-two-dimensional MHD simulation with a guide field, and a two-dimensional full dynamics kinetic simulation with inherent noise so that the apparent minimum gradient was not exactly zero, even without added artificial errors. We also examined variations of the spacecraft trajectory for the kinetic simulation. The accuracy of the directions found varied depending on the simulation and spacecraft trajectory, but all the directions could be found within about 10 for all cases. Various aspects of the method were examined, including how to choose averaging intervals and the best intervals for determining the directions and velocity. For the kinetic simulation, we also investigated in detail how the errors in the inferred gradient directions from the unmodified Shi et al. method (using the unperturbed gradient

  5. Study on the application of “Shi Di Jia”-soil conditioner on potato yield%“施地佳”土壤调理剂对马铃薯产量的影响

    Institute of Scientific and Technical Information of China (English)

    杨洋; 李智强; 刘军; 李越; 姚虞; 高娃; 孙国良

    2015-01-01

    In order to ex plore whether farmland nutrient utilization of “Shi Di Jia”-soil conditioner was improved, a field experiment was carried out in the application of “Shi Di Jia”on planting potato arable land. To clear out the effect of “Shi Di Jia”-soil conditioner on potato yield, the results showed that the use of “Shi Di Jia”-soil conditioner increased potato yield effect significantly.%为了探明“施地佳”土壤调理剂对耕地的养分利用是否具有改善作用,在施用“施地佳”的耕地进行种植马铃薯的田间试验,明确“施地佳”土壤调理剂对马铃薯产量的影响,结果表明施用“施地佳”土壤调理剂后马铃薯增产效果显著。

  6. Prevalence of swallowing and speech problems in daily life after chemoradiation for head and neck cancer based on cut-off scores of the patient-reported outcome measures SWAL-QOL and SHI

    NARCIS (Netherlands)

    Rinkel, Rico N.; Verdonck-de Leeuw, Irma M.; Doornaert, Patricia; Buter, Jan; de Bree, Remco; Langendijk, Johannes A.; Aaronson, Neil K.; Leemans, C. Rene

    The objective of this study is to assess swallowing and speech outcome after chemoradiation therapy for head and neck cancer, based on the patient-reported outcome measures Swallowing Quality of Life Questionnaire (SWAL-QOL) and Speech Handicap Index (SHI), both provided with cut-off scores. This is

  7. Prevalence of swallowing and speech problems in daily life after chemoradiation for head and neck cancer based on cut-off scores of the patient-reported outcome measures SWAL-QOL and SHI

    NARCIS (Netherlands)

    Rinkel, R.N.; Verdonck-de Leeuw, I.M.; Doornaert, P.; Buter, J.; de Bree, R.; Langendijk, J.A.; Aaronson, N.K.; Leemans, C.R.

    2016-01-01

    The objective of this study is to assess swallowing and speech outcome after chemoradiation therapy for head and neck cancer, based on the patient-reported outcome measures Swallowing Quality of Life Questionnaire (SWAL-QOL) and Speech Handicap Index (SHI), both provided with cut-off scores. This is

  8. Commentary of Hu Yihong' s Animation Theory%胡依红动画理论述评

    Institute of Scientific and Technical Information of China (English)

    屈立丰

    2012-01-01

    本文对上海美影厂女动画家胡依红的动画理论思想与动画创作实践做了简单梳理,细致分析了其“动画创新论”和“动画本体论”的理论来源和框架构成,对其动画理论思想和动画实践之间的互动关系进行了初步讨论。%This paper gave a brief introduction of the animation theory and creations by Hu Yihong, a female animator from Shanghai Animation Film Studio, made a detailed analysis of the sources and framework of her "ani- mation innovation theory" and "animation ontology", and finally presented a tentative discussion about the interac- tion between her ideology of animation theory and animation creation.

  9. A New Arthropod, Guangweicaris Luo, Fu et Hu gen. nov.from the Early Cambrian Guanshan Fauna, Kunming, China

    Institute of Scientific and Technical Information of China (English)

    LUO Huilin; FU Xiaoping; HU Shixue; LI Yong; HOU Shuguang; YOU Ting; PANG Jiyuan; LIU Qi

    2007-01-01

    The Guanshan Fauna is a soft-bodied fauna dominated by arthropods (including trilobites,trilobitoides, Tuzoia, Isoxys, and bradorids) in association with priapulids, brachiopods,anomalocaridids, vetulicoliids, sponges, chancellorids, and echinoderms. This paper reports and describes a new arthropod from the yellowish green mudstone at the lower part of the Wulongqing Formation, Canglangpuan Stage, Lower Cambrian in Kunming, Yunnan, China. The stratigraphic and geographic distribution, classification, fossil preservation, life style of this new arthropod and comparisons with other fossil arthropods are also discussed in details. The discovery and research of the non-mineralized arthropod, Guangweicaris Luo, Fu et Hu gen. nov. from the Guanshan Fauna adds new members to the taxonomic list and provides new information to the evolution of early arthropods.Furthermore, this study would shed new light into the "Cambrian Explosion" and the evolution of early life.

  10. Chinese President Hu Jintao and Austrian President Heinz Fischer Send Messages of Congratulations on 2006 China Year in Austria

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    <正>Editor’s note: In January 2004, Benita Ferrero-Waldner, Austrian foreign minister at the time, announced that China Year would be held in Austria in 2006 to mark the 35th anniversary of the establishment of diplomatic relations between Austria and China and to promote understanding of China and bilateral cooperation. The two sides decided after consultation that the activities for the China Year would be jointly organized by the Austria-China Friendship Association and the Chinese People’s Association for Friendship with Foreign Countries. The governments of the two countries attach great importance to the holding of the China Year. Chinese President Hu Jintao, Austrian President Heinz Fischer and Federal Chancellor Wolfgang Schussel and other state leaders

  11. Effects of vesicular-arbuscular mycorrhizae on the drought resistance of wild jujube (Zizyphs spinosus Hu) seedlings

    Institute of Scientific and Technical Information of China (English)

    LU Jinying; LIU Min; MAO Yongmin; SHEN Lianying

    2007-01-01

    The current study explored the effects of vesicular-arbuscular mycorrhizae (VAM) inoculation on the growth and water requirement of pot-grown wild jujube (Zizyphs spinosus Hu).Three water regimes (20%,40% and 60% of soil water content) were conducted.The VAM inoculation could significantly increase plant growth (including plant height,leaf area,and fresh and dry mass),enhance relative leaf water content,photosynthetic rates,transpiration rates and stomatal conductance,and improve plant drought tolerance.The water consumption of the mycorrhizal plants producing 1 g of dry matter was 18.7%-26.6% lower than the consumption of non-mycorrhizal plants grown under the same soil water content conditions.

  12. A computational screen for site selective A-to-I editing detects novel sites in neuron specific Hu proteins

    Directory of Open Access Journals (Sweden)

    Furey Terrence S

    2010-01-01

    Full Text Available Abstract Background Several bioinformatic approaches have previously been used to find novel sites of ADAR mediated A-to-I RNA editing in human. These studies have discovered thousands of genes that are hyper-edited in their non-coding intronic regions, especially in alu retrotransposable elements, but very few substrates that are site-selectively edited in coding regions. Known RNA edited substrates suggest, however, that site selective A-to-I editing is particularly important for normal brain development in mammals. Results We have compiled a screen that enables the identification of new sites of site-selective editing, primarily in coding sequences. To avoid hyper-edited repeat regions, we applied our screen to the alu-free mouse genome. Focusing on the mouse also facilitated better experimental verification. To identify candidate sites of RNA editing, we first performed an explorative screen based on RNA structure and genomic sequence conservation. We further evaluated the results of the explorative screen by determining which transcripts were enriched for A-G mismatches between the genomic template and the expressed sequence since the editing product, inosine (I, is read as guanosine (G by the translational machinery. For expressed sequences, we only considered coding regions to focus entirely on re-coding events. Lastly, we refined the results from the explorative screen using a novel scoring scheme based on characteristics for known A-to-I edited sites. The extent of editing in the final candidate genes was verified using total RNA from mouse brain and 454 sequencing. Conclusions Using this method, we identified and confirmed efficient editing at one site in the Gabra3 gene. Editing was also verified at several other novel sites within candidates predicted to be edited. Five of these sites are situated in genes coding for the neuron-specific RNA binding proteins HuB and HuD.

  13. Validation of the SCID-hu Thy/Liv mouse model with four classes of licensed antiretrovirals.

    Directory of Open Access Journals (Sweden)

    Cheryl A Stoddart

    Full Text Available BACKGROUND: The SCID-hu Thy/Liv mouse model of HIV-1 infection is a useful platform for the preclinical evaluation of antiviral efficacy in vivo. We performed this study to validate the model with representatives of all four classes of licensed antiretrovirals. METHODOLOGY/PRINCIPAL FINDINGS: Endpoint analyses for quantification of Thy/Liv implant viral load included ELISA for cell-associated p24, branched DNA assay for HIV-1 RNA, and detection of infected thymocytes by intracellular staining for Gag-p24. Antiviral protection from HIV-1-mediated thymocyte depletion was assessed by multicolor flow cytometric analysis of thymocyte subpopulations based on surface expression of CD3, CD4, and CD8. These mice can be productively infected with molecular clones of HIV-1 (e.g., the X4 clone NL4-3 as well as with primary R5 and R5X4 isolates. To determine whether results in this model are concordant with those found in humans, we performed direct comparisons of two drugs in the same class, each of which has known potency and dosing levels in humans. Here we show that second-generation antiretrovirals were, as expected, more potent than their first-generation predecessors: emtricitabine was more potent than lamivudine, efavirenz was more potent than nevirapine, and atazanavir was more potent than indinavir. After interspecies pharmacodynamic scaling, the dose ranges found to inhibit viral replication in the SCID-hu Thy/Liv mouse were similar to those used in humans. Moreover, HIV-1 replication in these mice was genetically stable; treatment of the mice with lamivudine did not result in the M184V substitution in reverse transcriptase, and the multidrug-resistant NY index case HIV-1 retained its drug-resistance substitutions. CONCLUSION: Given the fidelity of such comparisons, we conclude that this highly reproducible mouse model is likely to predict clinical antiviral efficacy in humans.

  14. Dieta de roedores sigmodontinos (Cricetidae en los bosques montanos tropicales de Huánuco, Perú

    Directory of Open Access Journals (Sweden)

    Maggie C. Noblecilla

    2013-06-01

    Full Text Available Se analizó el contenido estomacal de cinco especies de roedores sigmodontinos: Akodon orophilus, Microry-zomys altissimus, M. minutus, Thomasomys notatus yT. kalinowskii, procedentes de los bosques montanos de Huánuco, Perú (2564 - 3850 m de altitud. Concluimos que A. orophiluses insectívora por haber presentado un alto volumen de artrópodos (adultos y larvas en el contenido estomacal (90,1%; mientras que T. notatusy T. kalinowskiison principalmente herbívoras por el alto volumen de materia vegetal, 89% y 67,75% respectiva-mente; y que M. altissimusy M. minutusson omnívoras por presentar volúmenes similares tanto para vegetales como para artrópodos. Thomasomys kalinowskiies considerada generalista por la mayor amplitud de nicho (4,61, mientras A. orophiluses considerada especialista por el menor valor (1,70. Akodon orophilusmostró una preferencia por el consumo de artrópodos adultos al tener un bajo coeficiente de variación (CV= 20% y también un significativo aumento en el consumo de larvas de artrópodos en la época húmeda, siendo la única especie con variación estacional en la dieta. Por otro lado, la sobreposición de nicho fue menor a 0,75 en el 80% de los pares de especies comparados, indicando una baja similitud en la dieta. La mayor similitud en la dieta se observó entre M. altissimus- T. notatus(0,822 y M. minutus - T. kalinowskii(0,816. Se concluye que estos roedores sigmodontinos, simpátricos en los bosques montanos de Huánuco, exhiben dietas disímiles, probablemente como una estrategia para evitar o aminorar la competencia interespecífica.

  15. Validation of the SCID-hu Thy/Liv mouse model with four classes of licensed antiretrovirals.

    Science.gov (United States)

    Stoddart, Cheryl A; Bales, Cheryl A; Bare, Jennifer C; Chkhenkeli, George; Galkina, Sofiya A; Kinkade, April N; Moreno, Mary E; Rivera, José M; Ronquillo, Rollie E; Sloan, Barbara; Black, Paul L

    2007-08-01

    The SCID-hu Thy/Liv mouse model of HIV-1 infection is a useful platform for the preclinical evaluation of antiviral efficacy in vivo. We performed this study to validate the model with representatives of all four classes of licensed antiretrovirals. Endpoint analyses for quantification of Thy/Liv implant viral load included ELISA for cell-associated p24, branched DNA assay for HIV-1 RNA, and detection of infected thymocytes by intracellular staining for Gag-p24. Antiviral protection from HIV-1-mediated thymocyte depletion was assessed by multicolor flow cytometric analysis of thymocyte subpopulations based on surface expression of CD3, CD4, and CD8. These mice can be productively infected with molecular clones of HIV-1 (e.g., the X4 clone NL4-3) as well as with primary R5 and R5X4 isolates. To determine whether results in this model are concordant with those found in humans, we performed direct comparisons of two drugs in the same class, each of which has known potency and dosing levels in humans. Here we show that second-generation antiretrovirals were, as expected, more potent than their first-generation predecessors: emtricitabine was more potent than lamivudine, efavirenz was more potent than nevirapine, and atazanavir was more potent than indinavir. After interspecies pharmacodynamic scaling, the dose ranges found to inhibit viral replication in the SCID-hu Thy/Liv mouse were similar to those used in humans. Moreover, HIV-1 replication in these mice was genetically stable; treatment of the mice with lamivudine did not result in the M184V substitution in reverse transcriptase, and the multidrug-resistant NY index case HIV-1 retained its drug-resistance substitutions. Given the fidelity of such comparisons, we conclude that this highly reproducible mouse model is likely to predict clinical antiviral efficacy in humans.

  16. Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjögren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model.

    Science.gov (United States)

    Gervais, Elise M; Desantis, Kara A; Pagendarm, Nicholas; Nelson, Deirdre A; Enger, Tone; Skarstein, Kathrine; Liaaen Jensen, Janicke; Larsen, Melinda

    2015-09-01

    Sjögren's syndrome (SS), an autoimmune exocrinopathy, is associated with dysfunction of the secretory salivary gland epithelium, leading to xerostomia. The etiology of SS disease progression is poorly understood as it is typically not diagnosed until late stage. Since mouse models allow the study of disease progression, we investigated the NOD/ShiLtJ mouse to explore temporal changes to the salivary epithelium. In the NOD/ShiLtJ model, SS presents secondary to autoimmune diabetes, and SS disease is reportedly fully established by 20 weeks. We compared epithelial morphology in the submandibular salivary glands (SMG) of NOD/ShiLtJ mice with SMGs from the parental strain at 12, 18, and 22 weeks of age and used immunofluorescence to detect epithelial proteins, including the acinar marker, aquaporin 5, ductal cell marker, cytokeratin 7, myoepithelial cell marker, smooth muscle α-actin, and the basal cell marker, cytokeratin 5, while confirming immune infiltrates with CD45R. We also compared these proteins in the labial salivary glands of human SS patients with control tissues. In the NOD/ShiLtJ SMG, regions of lymphocytic infiltrates were not associated with widespread epithelial tissue degradation; however, there was a decrease in the area of the gland occupied by secretory epithelial cells in favor of ductal epithelial cells. We observed an expansion of cells expressing cytokeratin 5 within the ducts and within the smooth muscle α-actin(+) basal myoepithelial population. The altered acinar/ductal ratio within the NOD/ShiLtJ SMG likely contributes to salivary hypofunction, while the expansion of cytokeratin 5 positive-basal cells may reflect loss of function or indicate a regenerative response.

  17. Measurement of parameters of scintillating bars with wavelength-shifting fibres and silicon photomultiplier readout for the SHiP Muon Detector

    Science.gov (United States)

    Baldini, W.; Blondel, A.; Calcaterra, A.; Jacobsson, R.; Khotjantsev, A.; Kudenko, Y.; Kurochka, V.; Lanfranchi, G.; Mefodiev, A.; Mineev, O.; Montanari, A.; Noah Messomo, E.; Saputi, A.; Tosi, N.

    2017-03-01

    The light yield and the time resolution of different types of 3 m long scintillating bars instrumented with wavelength shifting fibres and read out by different models of silicon photomultipliers have been measured at a test beam at the T9 area at the CERN Proton Synchrotron. The results obtained with different configurations are presented. A time resolution better than 800 ps, constant along the bar length within 20%, and a light yield of 0~ 14 (70) photoelectrons are obtained for bars 3 m long, ~ 4.5 (5) cm wide and 2 (0.7) cm thick. These results nicely match the requirements for the Muon Detector of the SHiP experiment.

  18. Measurement of parameters of scintillating bars with wavelength-shifting fibres and silicon photomultiplier readout for the SHiP Muon Detector

    CERN Document Server

    Montanari, Alessandro; Baldini, Wander; Calcaterra, Alessandro; Lanfranchi, Gaia; Saputi, Alessandro; Khotyantsev, Alexey; Kudenko, Yury; Mefodev, Aleksandr; Mineev, Oleg

    2016-01-01

    The light yield and the time resolution of different types of 3 m long scintillating bars instrumented with wavelength shifting fibres and read out by different models of silicon photomultipliers have been measured at a test beam at the T9 area at the CERN Proton Synchrotron. The results obtained with different configurations are presented. A time resolution better than 800 ps, constant along the bar length within 20%, and a light yield of 140 (70) photo-electrons are obtained for bars 3 m long, 4.5 (5) cm wide and 2 (0.7) cm thick. These results nicely match the requirements for the Muon Detector of the SHiP experiment.

  19. The Analysis of YE Tian-shi's Experience in Clinical Treatment by Using Lobster Sauce%叶天士运用豆豉经验浅析

    Institute of Scientific and Technical Information of China (English)

    汪春红; 陈烨文; 陈一江

    2014-01-01

    Lobster sauce is widely used in Chinese classical prescriptions ,while now is seldom used again .For mastering the thought of using lobster sauce by Zhang Zhong -jing, the methods of researching Ye Tian -shi's works and medical cases is used , to explore lob-ster sauce's efficiency , which should be good for clinical TCM doctors'prescriptions nowadays .%豆豉在经方中应用广泛,今却已鲜为人所用。以研究叶天士的《临证指南医》中的医案为手段发掘豆豉的临床使用证候,使经方更好的运用于临床。

  20. Stratigraphic framework and coal correlation of the Upper Cretaceous Fruitland Formation, Bisti-Ah-Shi-Sle-Pah area, San Juan Basin, New Mexico

    Science.gov (United States)

    Flores, Romeo M.; Erpenbeck, Michael F.

    1982-01-01

    This report illustrates and describes the detailed stratigraphic framework and coal correlation of the Upper Cretaceous Fruitland Formation exposed in isolated badlands and along washes within a 20-mile outcrop belt in the Bisti-Ah-Shi-Sle-Pah area, southwestern San Juan Basin, Nex Mexico (see index). The stratigraphic framework showing the vertical and lateral distributions of rock types and the lateral continuity of coal beds is illustrated in cross sections. The cross sections were constructed from 112 stratigraphic sections measured at an average distance of 0.4 mi apart. Each section contained key marker beds (sandstone, coal, and tonstein) that were physically traced to adjacent sections. Each measured section was "hung" on multiple marker beds arranged in a geometric best-fit method that accounts for the differential compaction and facies associations of the deposits. 

  1. WEN Jian-min and SHI Da-zhuo : ICWM Should Be Taken as A Discipline Uniformly and The Practice Scope of ICWM Practitioners Be Defined

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ According to the current inconsistent status of subject names and stratification of ICWM and based on the principle of classification that a subject can only use one name and one code, Prof. WEN Jian-min and Prof. SHI Da-zhuo, two CPPCC members, proposed that ICWM in the subject categories of National Standardization Committee (defined as a subdiscipline under CM and Chinese materia medica) and that in the subject categories of the Academic Degree Commission of the State Council (defined as a discipline, consisting of two sub-disciplines, the basic ICWM and the clinical ICWM) should be taken as a discipline uniformly, consisting of 12 subdisciplines, Basic Medicine, Clinical Medicine, Diagnostics, Internal Medicine, Surgery, Orthopaedics, Pediatrics,Ophthalmology, Otorhinolaryngology, Dermatology and Venereology, Obstetrics and Gynecology, and Other Subjects.

  2. Light dark matter in neutrino beams: Production modeling and scattering signatures at MiniBooNE, T2K, and SHiP

    Science.gov (United States)

    deNiverville, Patrick; Chen, Chien-Yi; Pospelov, Maxim; Ritz, Adam

    2017-02-01

    We analyze the prospects for detection of light sub-GeV dark matter produced in experiments designed to study the properties of neutrinos, such as MiniBooNE, T2K, SHiP, DUNE etc. We present an improved production model, when dark matter couples to hadronic states via a dark photon or baryonic vector mediator, incorporating bremsstrahlung of the dark vector. In addition to elastic scattering, we also study signatures of light dark matter undergoing deep inelastic or quasielastic NC π0 -like scattering in the detector producing neutral pions, which for certain experiments may provide the best sensitivity. Supplemental Material provides extensive documentation for a publicly available simulation tool BdNMC that can be applied to determine the hidden sector dark matter production and scattering rate at a range of proton fixed target experiments.

  3. Light dark matter in neutrino beams: production modelling and scattering signatures at MiniBooNE, T2K and SHiP

    CERN Document Server

    deNiverville, Patrick; Pospelov, Maxim; Ritz, Adam

    2016-01-01

    We analyze the prospects for detection of light sub-GeV dark matter produced in experiments designed to study the properties of neutrinos, such as MiniBooNE, T2K, SHiP, DUNE etc. We present an improved production model, when dark matter couples to hadronic states via a dark photon or baryonic vector mediator, incorporating bremsstrahlung of the dark vector. In addition to elastic scattering, we also study signatures of light dark matter undergoing deep inelastic or quasi-elastic NC$\\pi^0$-like scattering in the detector producing neutral pions, which for certain experiments may provide the best sensitivity. An extensive appendix provides documentation for a publicly available simulation tool {\\tt BdNMC} that can be applied to determine the hidden sector dark matter production and scattering rate at a range of proton fixed target experiments.

  4. Zhou Bida’s Methodology of Commenting on Si Shi%周必大评苏的方法问题

    Institute of Scientific and Technical Information of China (English)

    杨胜宽

    2014-01-01

    周必大作为南宋前期重要的政治家,在当时朝野推崇苏学的政治背景和学术氛围下,十分关注与敬重苏轼。他采用考证、点评、辨析等方法,对苏轼的生平事迹、道德文章进行了深入考察和客观评价,体现了作者独特的人品风范与学问见识。文章对这些问题进行了系统梳理和总结概括。周必大提供的不少今人未见的苏轼佚文线索,表明苏轼研究的文献收集整理等基础工作,还需要继续努力进行。%Zhou Bida is an important politician during the former period of the Southern Song Dynasty, who paid much attention to and respected Su Shi under the circumstances of the political background and a-cademic atmosphere in the court and commonalty. He used many methods to observe and critique Su Shi’s lifetime achievements and moral articles in depth, such as textual research, commenting and discrimination, embodying his great personality and academic achievements. This essay systematically analyzes and summa-rizes what have been mentioned above. Many of the leads that Zhou Bida provided for researches on Su Shi are quite precious, indicating that more efforts should be made to the fundamental work of collecting and restoring relevant literature.

  5. Text Analysis of Yaksha by SHI Zhe -cun%施蛰存短篇小说《夜叉》文本分析

    Institute of Scientific and Technical Information of China (English)

    谷晓曦

    2012-01-01

    Yaksha by SHI Zhe cun is a very special novel written in Shanghai in the 1930s. SHI Zhe cun is one of the representative writers of neosensuatist novels in the history of Chinese modern literature. He does creatively in literature style and literary ideas in his'works and he is the first to recognize the modernists need to have Chinese characteristics. Yaksha is influenced by Chi nese classical literature and western modernists, however, the author does not copy these experi ences and he is creative in style and ideas based on the features of the times, making the novel a successful practice of his theory.%施蛰存是中国现代文学史上新感觉派的代表作家,他的文学手法以及文学观念在作品中都有突出表现,并且他是最早认识到现代派需要有中国特色的一个作家。其短篇小说《夜叉》受到了中国古典文学的影响,同时又融入了许多西方现代派的思想,然而作者并不照搬这些经验,而是结合当时的时代背景,在继承的基础上有所创新,使得《夜叉》成为他创作理论的成功实践。

  6. Infección por Gnathostoma (Spirurida: Gnathostomatidae en Hoplias microlepis: prevalencia, correlación con la talla del pez, huéspedes e implicaciones para salud pública en Ecuador

    Directory of Open Access Journals (Sweden)

    Juan José Alava

    2009-12-01

    Conclusiones. La gnathostomiasis sigue siendo prevalente en la zona costera de Ecuador y el agente patógeno es aún encontrado en el huésped intermediario. Diversos mamíferos neotropicales estarían actuando como huésped reservorio definitivo en el ciclo biológico de Gnathostoma en Ecuador.

  7. HuCNS-SC Human NSCs Fail to Differentiate, Form Ectopic Clusters, and Provide No Cognitive Benefits in a Transgenic Model of Alzheimer's Disease

    Directory of Open Access Journals (Sweden)

    Samuel E. Marsh

    2017-02-01

    Full Text Available Transplantation of neural stem cells (NSCs can improve cognition in animal models of Alzheimer's disease (AD. However, AD is a protracted disorder, and prior studies have examined only short-term effects. We therefore used an immune-deficient model of AD (Rag-5xfAD mice to examine long-term transplantation of human NSCs (StemCells Inc.; HuCNS-SCs. Five months after transplantation, HuCNS-SCs had engrafted and migrated throughout the hippocampus and exhibited no differences in survival or migration in response to β-amyloid pathology. Despite robust engraftment, HuCNS-SCs failed to terminally differentiate and over a quarter of the animals exhibited ectopic human cell clusters within the lateral ventricle. Unlike prior short-term experiments with research-grade HuCNS-SCs, we also found no evidence of improved cognition, no changes in brain-derived neurotrophic factor, and no increase in synaptic density. These data, while disappointing, reinforce the notion that individual human NSC lines need to be carefully assessed for efficacy and safety in appropriate long-term models.

  8. Short communication. Employment of molecular markers to develop tetraploid “supermale” asparagus from andromonoecious plants of the landrace ‘Morado de Huétor’

    Directory of Open Access Journals (Sweden)

    Jose J. Regalado

    2014-10-01

    Full Text Available The aim of this work was the development of new “supermale” genotypes retaining the highest genetic diversity possible of the tetraploid asparagus landrace ‘Morado de Huétor’. The elite andromonoecious male HT664 of the ‘Morado de Huétor’ asparagus landrace and the andromonoecious hybrid male HC027, obtained by crossing between this landrace and a commercial cultivar of Asparagus officinalis, were selected for self-pollination to produce possible “supermales” with genes of ‘Morado de Huétor’ (SMHT. To confirm the hybrid nature of HC027, we characterized this genotype with EST-SSR (Expressed Sequence Tag-derived Simple Sequence Repeats markers. We also adopted the sex-linked marker Asp1-T7 for sex determination in ‘Morado de Huétor’ and the resulting hybrids between this landrace and other commercial cultivars. Asp1-T7 marker was used for the selection and genotyping of SMHT. “All-male” cultivars with a different genetic background can be generated by crossing females with these new “supermale” genotypes, and the agronomical traits of these new cultivars would be very different from the “all-male” cultivars currently available in the market, making them extremely interesting for asparagus breeding programs.

  9. On the Theoretical Character of Hu Jintao's Youth Viewpoint%胡锦涛同志青年观的理论品格思想研究

    Institute of Scientific and Technical Information of China (English)

    邹洪伟

    2009-01-01

    Mr. Hu Jintao has delivered lots pithy opinions on the youth work and the healthy growth of Chi-nese youths, with which Mr. Hu has developed the Marxist youth theory to its new level. This paper makes a brief illustration of the guidelines, the kernel, the goal and the historical mission of the youth work and the healthy growth of Chinese youths mentioned by Mr. Hu, trying to discuss the theoretical character of Mr. Hu Jintao's youth viewpoint.%胡锦涛同志对中国青年成长成才和青年工作提出了诸多精辟的观点,开拓了马克思主义青年观的新境界.本文就其中青年成长成才和青年工作的指导思想、核心、努力目标和历史使命等问题进行了简要阐述,充分展现胡锦涛同志青年观的理论品格.

  10. Purchase of Microwave Reactors for Implementation of Small-scale Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate Curriculum and Synthetic Chemistry Research at HU

    Science.gov (United States)

    2015-05-16

    Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate Curriculum and Synthetic Chemistry Research at HU The views, opinions and/or...Research Triangle Park, NC 27709-2211 Microwave laboratory facility, Undergraduate organic chemistry laboratory, Implementation/Development of...Purchase of Microwave Reactors for Implementation of Small-scale Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate

  11. HuD promotes BDNF expression in brain neurons via selective stabilization of the BDNF long 3'UTR mRNA.

    Directory of Open Access Journals (Sweden)

    Megan Allen

    Full Text Available Complex regulation of brain-derived neurotrophic factor (BDNF governs its intricate functions in brain development and neuronal plasticity. Besides tight transcriptional control from multiple distinct promoters, alternative 3'end processing of the BDNF transcripts generates either a long or a short 3'untranslated region (3'UTR. Previous reports indicate that distinct RNA sequence in the BDNF 3'UTRs differentially regulates BDNF production in the brain to accommodate neuronal activity changes, conceivably through differential interactions with undefined trans-acting factors that regulate stability and translation of these BDNF mRNA isoforms. In this study, we report that the neuronal RNA-binding protein (RBP HuD interacts with a highly conserved AU-rich element (ARE specifically located in the BDNF long 3'UTR. Such interaction is necessary and sufficient for selective stabilization of mRNAs that contain the BDNF long 3'UTR in vitro and in vivo. Moreover, in a HuD transgenic mouse model, the BDNF long 3'UTR mRNA is increased in the hippocampal dentate granule cells (DGCs, leading to elevated expression of BDNF protein that is transported and stored in the mossy fiber (MF terminals. Our results identify HuD as the first trans-acting factor that enhances BDNF expression specifically through the long 3'UTR and a novel mechanism that regulates BDNF protein production in selected neuronal populations by HuD abundance.

  12. The problem of the ethical root of truth (A tribute to Antonio Rodríguez Huéscar on his birth centenary

    Directory of Open Access Journals (Sweden)

    Juan Padilla

    2013-12-01

    Full Text Available This paper deals with the meaning of truth as «coincidence of man with himself» accordingto Ortega y Gasset and his disciple rodríguez huéscar. What the concept does not mean and which implications it has are also explained.

  13. PARP1 promotes gene expression at the post-transcriptional level by modulating the RNA-binding protein HuR

    Science.gov (United States)

    Ke, Yueshuang; Han, Yanlong; Guo, Xiaolan; Wen, Jitao; Wang, Ke; Jiang, Xue; Tian, Xue; Ba, Xueqing; Boldogh, Istvan; Zeng, Xianlu

    2017-01-01

    Poly(ADP-ribosyl)ation (PARylation) is mainly catalysed by poly-ADP-ribose polymerase 1 (PARP1), whose role in gene transcription modulation has been well established. Here we show that, in response to LPS exposure, PARP1 interacts with the adenylateuridylate-rich element-binding protein embryonic lethal abnormal vision-like 1 (Elavl1)/human antigen R (HuR), resulting in its PARylation, primarily at site D226. PARP inhibition and the D226 mutation impair HuR's PARylation, nucleocytoplasmic shuttling and mRNA binding. Increases in mRNA level or stability of pro-inflammatory cytokines/chemokines are abolished by PARP1 ablation or inhibition, or blocked in D226A HuR-expressing cells. The present study demonstrates a mechanism to regulate gene expression at the post-transcriptional level, and suggests that blocking the interaction of PARP1 with HuR could be a strategy to treat inflammation-related diseases that involve increased mRNA stability. PMID:28272405

  14. Priming with r-metHuSCF and filgrastim or chemotherapy and filgrastim in patients with malignant lymphomas: a randomized phase II pilot study of mobilization and engraftment

    DEFF Research Database (Denmark)

    Johnsen, Hanne; Geisler, C; Juvonen, E

    2011-01-01

    SCF has been shown to synergize with G-CSF to mobilize CD34(+) PBPCs. In this study we report results from this combination after a phase II trial of 32 patients with malignant lymphoma randomized to receive recombinant methionyl human SCF (ancestim, r-metHuSCF) in combination with recombinant me...

  15. Investigation of the HU-density conversion method and comparison of dose distribution for dose calculation on MV cone beam CT images

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Min Joo; Lee, Seu Ran; Suh, Tae Suk [Dept. of Biomedical Engineering, The Catholic University of Korea, Bucheon (Korea, Republic of)

    2011-11-15

    Modern radiation therapy techniques, such as Image-guided radiation therapy (IGRT), Adaptive radiation therapy (ART) has become a routine clinical practice on linear accelerators for the increase the tumor dose conformity and improvement of normal tissue sparing at the same time. For these highly developed techniques, megavoltage cone beam computed tomography (MVCBCT) system produce volumetric images at just one rotation of the x-ray beam source and detector on the bottom of conventional linear accelerator for real-time application of patient condition into treatment planning. MV CBCT image scan be directly registered to a reference CT data set which is usually kilo-voltage fan-beam computed tomography (kVFBCT) on treatment planning system and the registered image scan be used to adjust patient set-up error. However, to use MV CBCT images in radiotherapy, reliable electron density (ED) distribution are required. Patients scattering, beam hardening and softening effect caused by different energy application between kVCT, MV CBCT can cause cupping artifacts in MV CBCT images and distortion of Houns field Unit (HU) to ED conversion. The goal of this study, for reliable application of MV CBCT images into dose calculation, MV CBCT images was modified to correct distortion of HU to ED using the relationship of HU and ED from kV FBCT and MV CBCT images. The HU-density conversion was performed on MV CBCT image set using Dose difference map was showing in Figure 1. Finally, percentage differences above 3% were reduced depending on applying density calibration method. As a result, total error co uld be reduced to under 3%. The present study demonstrates that dose calculation accuracy using MV CBCT image set can be improved my applying HU-density conversion method. The dose calculation and comparison of dose distribution from MV CBCT image set with/without HU-density conversion method was performed. An advantage of this study compared to other approaches is that HU

  16. Detrimental ELAVL-1/HuR-dependent GSK3β mRNA stabilization impairs resolution in acute respiratory distress syndrome

    Science.gov (United States)

    Hoffman, Olivia; Burns, Nana; Vadász, István; Eltzschig, Holger K.; Edwards, Michael G.

    2017-01-01

    A hallmark of acute respiratory distress syndrome (ARDS) is accumulation of protein-rich edema in the distal airspaces and its removal is critical for patient survival. Previous studies have shown a detrimental role of Glycogen Synthase Kinase (GSK) 3β during ARDS via inhibition of alveolar epithelial protein transport. We hypothesized that post-transcriptional regulation of GSK3β could play a functional role in ARDS resolution. To address this hypothesis, we performed an in silico analysis to identify regulatory genes whose expression correlation to GSK3β messenger RNA utilizing two lung cancer cell line array datasets. Among potential regulatory partners of GSK3β, these studies identified the RNA-binding protein ELAVL-1/HuR (Embryonic Lethal, Abnormal Vision, Drosophila-Like) as a central component in a likely GSK3β signaling network. ELAVL-1/HuR is a RNA-binding protein that selectively binds to AU-rich elements of mRNA and enhances its stability thereby increasing target gene expression. Subsequent studies with siRNA suppression of ELAVL-1/HuR demonstrated deceased GSK3β mRNA and protein expression and improved clearance of FITC-albumin in A549 cells. Conversely, stabilization of ELAVL-1/HuR with the proteasome inhibitor MG-132 resulted in induction of GSK3β at mRNA and protein level and attenuated FITC-albumin clearance. Utilizing ventilator-induced lung injury or intra-tracheal installation of hydrochloric acid to induce ARDS in mice, we observed increased mRNA and protein expression of ELAVL-1/HuR and GSK3β. Together, our findings indicate a previously unknown interaction between GSK3β and ELAV-1 during ARDS, and suggest the inhibition of the ELAV-1- GSK3β pathways as a novel ARDS treatment approach. PMID:28196122

  17. The Anti-Inflammatory Effect and Intestinal Barrier Protection of HU210 Differentially Depend on TLR4 Signaling in Dextran Sulfate Sodium-Induced Murine Colitis.

    Science.gov (United States)

    Lin, Sisi; Li, Yongyu; Shen, Li; Zhang, Ruiqin; Yang, Lizhi; Li, Min; Li, Kun; Fichna, Jakub

    2017-02-01

    Ulcerative colitis (UC) is strongly associated with inflammation and intestinal barrier disorder. The nonselective cannabinoid receptor agonist HU210 has been shown to ameliorate inflamed colon in colitis, but its effects on intestinal barrier function and extraintestinal inflammation are unclear. To investigate the effects and the underlying mechanism of HU210 action on the UC in relation to a role of TLR4 and MAP kinase signaling. Wild-type (WT) and TLR4 knockout (Tlr4 (-/-)) mice were exposed to 4% dextran sulfate sodium (DSS) for 7 days. The effects of HU210 on inflammation and intestinal barrier were explored. Upon DSS challenge, mice suffered from bloody stool, colon shortening, intestinal mucosa edema, pro-inflammatory cytokine increase and intestinal barrier destruction with goblet cell depletion, increased intestinal microflora accompanied with elevated plasma lipopolysaccharide, reduced mRNA expression of the intestinal tight junction proteins, and abnormal ratio of CD4(+)/CD8(+) T cells in the intestinal Peyer's patches. Pro-inflammatory cytokines in the plasma and the lung, as well as pulmonary myeloperoxidase activity, indicators of extraintestinal inflammation were increased. Protein expression of p38α and pp38 was up-regulated in the colon of WT mice. Tlr4 (-/-) mice showed milder colitis. HU210 reversed the intestinal barrier changes in both strains of mice, but alleviated inflammation only in WT mice. Our study indicates that in experimental colitis, HU210 displays a protective effect on the intestinal barrier function independently of the TLR4 signaling pathway; however, in the extraintestinal tissues, the anti-inflammatory action seems through affecting TLR4-mediated p38 mitogen-activated protein kinase pathway.

  18. Cytoplasmic expression of HuR may be a valuable diagnostic tool for determining the potential for malignant transformation of oral verrucous borderline lesions

    Science.gov (United States)

    HABIBA, UMMA; KITAMURA, TETSUYA; YANAGAWA-MATSUDA, AYA; HIDA, KYOKO; HIGASHINO, FUMIHIRO; OHIRO, YOICHI; TOTSUKA, YASUNORI; SHINDOH, MASANOBU

    2014-01-01

    Oral verrucous carcinoma (OVC) is a low grade variant of oral squamous cell carcinoma, and oral verrucous hyperplasia (OVH) is a benign lesion without malignant features. However, pathologists are sometimes presented with borderline lesions and are indecisive as to diagnose them as benign or malignant. Thus, these lesions are tentatively termed oral verrucous lesions (OVLs). HuR is an ARE mRNA-binding protein, normally localized in the nucleus but cytoplasmic exportation is frequently observed in cancer cells. The present study aimed to elucidate whether expression of the HuR protein facilitates the diagnosis of true malignant lesions. Clinicopathological features were evaluated, and immunohistochemical analysis for p53, Ki67 and HuR proteins was performed in 48 cases of OVH, OVC and OVL, and the outcomes were correlated using appropriate statistical analysis. The association of these three proteins in relation to malignant transformation was analyzed after a 3-year follow-up of 25 OVL cases. The basal characteristics (age, gender and location) of all cases had no significant association with the types of lesions. Gingiva (39.4%) was the common site for all lesions. Distribution of the examined proteins had a significant association with the lesions. As compared with the OVLs, the number of immunostained-positive cells was significantly higher in the OVCs and lower in the OVH cases. During follow-up, 24% of the OVLs underwent malignant transformation for which high HuR expression and a diffuse staining pattern in the epithelium were observed. Taken together, the high degree of HuR expression with diffuse staining pattern in the epithelium may be an effective diagnostic tool that determines the potential of OVLs for malignant transformation. PMID:24534848

  19. Cytoplasmic expression of HuR may be a valuable diagnostic tool for determining the potential for malignant transformation of oral verrucous borderline lesions.

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Hida, Kyoko; Higashino, Fumihiro; Ohiro, Yoichi; Totsuka, Yasunori; Shindoh, Masanobu

    2014-04-01

    Oral verrucous carcinoma (OVC) is a low grade variant of oral squamous cell carcinoma, and oral verrucous hyperplasia (OVH) is a benign lesion without malignant features. However, pathologists are sometimes presented with borderline lesions and are indecisive as to diagnose them as benign or malignant. Thus, these lesions are tentatively termed oral verrucous lesions (OVLs). HuR is an ARE mRNA-binding protein, normally localized in the nucleus but cytoplasmic exportation is frequently observed in cancer cells. The present study aimed to elucidate whether expression of the HuR protein facilitates the diagnosis of true malignant lesions. Clinicopathological features were evaluated, and immunohistochemical analysis for p53, Ki67 and HuR proteins was performed in 48 cases of OVH, OVC and OVL, and the outcomes were correlated using appropriate statistical analysis. The association of these three proteins in relation to malignant transformation was analyzed after a 3-year follow-up of 25 OVL cases. The basal characteristics (age, gender and location) of all cases had no significant association with the types of lesions. Gingiva (39.4%) was the common site for all lesions. Distribution of the examined proteins had a significant association with the lesions. As compared with the OVLs, the number of immunostained-positive cells was significantly higher in the OVCs and lower in the OVH cases. During follow-up, 24% of the OVLs underwent malignant transformation for which high HuR expression and a diffuse staining pattern in the epithelium were observed. Taken together, the high degree of HuR expression with diffuse staining pattern in the epithelium may be an effective diagnostic tool that determines the potential of OVLs for malignant transformation.

  20. Interleukin 1β Regulation of the System xc- Substrate-specific Subunit, xCT, in Primary Mouse Astrocytes Involves the RNA-binding Protein HuR.

    Science.gov (United States)

    Shi, Jingxue; He, Yan; Hewett, Sandra J; Hewett, James A

    2016-01-22

    System xc(-) is a heteromeric amino acid cystine/glutamate antiporter that is constitutively expressed by cells of the CNS, where it functions in the maintenance of intracellular glutathione and extracellular glutamate levels. We recently determined that the cytokine, IL-1β, increases the activity of system xc(-) in CNS astrocytes secondary to an up-regulation of its substrate-specific light chain, xCT, and that this occurs, in part, at the level of transcription. However, an in silico analysis of the murine xCT 3'-UTR identified numerous copies of adenine- and uridine-rich elements, raising the possibility that undefined trans-acting factors governing mRNA stability and translation may also contribute to xCT expression. Here we show that IL-1β increases the level of mRNA encoding xCT in primary cultures of astrocytes isolated from mouse cortex in association with an increase in xCT mRNA half-life. Additionally, IL-1β induces HuR translocation from the nucleus to the cytoplasm. RNA immunoprecipitation analysis reveals that HuR binds directly to the 3'-UTR of xCT in an IL-1β-dependent manner. Knockdown of endogenous HuR protein abrogates the IL-1β-mediated increase in xCT mRNA half-life, whereas overexpression of HuR in unstimulated primary mouse astrocytes doubles the half-life of constitutive xCT mRNA. This latter effect is accompanied by an increase in xCT protein levels, as well as a functional increase in system xc(-) activity. Altogether, these data support a critical role for HuR in mediating the IL-1β-induced stabilization of astrocyte xCT mRNA.

  1. Type-Ⅰcannabinoid receptor mediates down-regulation of CXCR4 in astrocytes by cannabinoid HU210%Ⅰ型大麻素受体介导大麻素HU210对星型胶质细胞CXCR4水平下调

    Institute of Scientific and Technical Information of China (English)

    朱舟; 韩静; 张遐; 徐逸

    2016-01-01

    目的 探寻大麻素抑制中枢神经系统免疫反应的机制,为大麻素临床药物的合理应用提供实验依据.方法 用不同浓度的HU210刺激培养的星形胶质细胞,利用Western blot检测并比较刺激组和未刺激组细胞CXCR4蛋白水平的差异,进而用Ⅰ型大麻素受体(type-1 cannabinoid receptor,CB1R)拮抗剂AM281刺激细胞后,观察HU210对CXCR4表达的影响.结果 Western blot检测结果显示,高浓度HU210能下调星形胶质细胞CXCR4表达,AM28能阻断HU210所致的CXCR4下调.结论 HU210经由CB1R下调CXCR4,这可能是大麻发挥免疫抑制作用的机理之一.

  2. 胡适What I Believe及My Credo and Its Evolution与《四十自述》关系考辨%A Textual Study on Hu Shi's What I Believe and My Credo and Its Evolution and Accounts at Forty

    Institute of Scientific and Technical Information of China (English)

    张婷

    2010-01-01

    目前针对胡适少年时期思想的研究,通常使用的是胡适的及(What I Believe).论文通过及书信的记载,考察胡适的My Credo and Its Evolution与What I Believe的内容与成书时间,提出它们就是写作的最初蓝本.

  3. Is it a poison or a tonic?——A study on ancient Chinese Han Shi San%毒药还是良药?——中国古代寒食散探析

    Institute of Scientific and Technical Information of China (English)

    雷志华; 高策

    2012-01-01

    Han Shi San has been very popular at one time in history.Both nobles and common people went after this medicine.However,it is difficult to control the efficacy of Han Shi San.People taking it have different results.Some were saved,others were died.Most of modern scholars take the attitude of doubting about Han Shi San and even denying it.Based on the analysis of the minerals contained in Han Shi San,it is argued that the Zi Shiying contained in Han Shi San is not amethyst but fluoritum.It is calcium fluoride,not silicon dioxide.Excessive dosage of calcium fluoride may result in fluorosis.Han Shi San is both a poison and a tonic because of the trace elements it contained.These trace elements such as fluorine,arsenic are good to health when used properly.However,overdosing of them is poisonous.%寒食散在我国历史上一度很流行,上至帝王将相,下至平民百姓,趋之若鹜。寒食散的疗效难以控制,服食者中有绝处逢生的,也有命丧黄泉的。近现代学者对其多持批判否定态度。通过对寒食散所用矿物药进行分析,我们提出:寒食散中的紫石英是萤石(CaF2),而不是过去认为的水晶(SiO2),过量摄入它会引起氟中毒;寒食散既是"毒药"又是"良药",原因在于矿物用药中所含的氟、砷等微量元素,适量的微量元素对人体有益,过量摄入则会造成中毒。

  4. Brief Explanation of"Shi"of Bone in Qi Xiao Liang Fang(《奇效良方》Prescriptions of Wonderful Efficacy)%《奇效良方》骨之“势”浅解

    Institute of Scientific and Technical Information of China (English)

    郭玉晶; 陈居伟; 刘更生

    2012-01-01

    《奇效良方·正骨兼金镞门》是古代伤科文献中的重要资料,其所记载的人身“三百六十五骨节”中包括对于骨内物质“势、液、髓”的论述.这一论述在现存古代文献记载中较为少见,但它也清楚的表明古人对于人体构造是有过系统研究的,且观察比较仔细.文章对《奇效良方·正骨兼金镞门》所载“势、液、髓”中“势”进行了研究,通过“势”字的含义演变结合现代人体解剖中骨骼构造的研究进行系统分析,认为“势、液、髓”中“势”所指代相当于现代骨骼构造中骨小梁及部分骨密质结构.综上,文章意在引起中医学界对于中医学人体解剖生理的重视.%Volume 56 of Qi Xiao Liang Fang () Prescriptions of Wonderful Efficacy) ( ) is the essential material among the ancient literatures of traumatology. It records "three hundred and sixty - five joints"of the human body and discusses"Shi,Ye,Sui" ,the substances of bone. The statement is seldom seen in the present existing ancient documents, but it clearly indicates the systematic study of the human body structure and the intensive observation by the ancients. This paper focuses on the study on the understanding of" Shi" recorded in" Shi, Ye, Sui "of the Volume 56 of Qi Xiao Liang Fang (Prescriptions of Wonderful Efficacy) (). A systematic analysis was conducted through the study on the evolution on the meaning of" Shi" in combination with the skeleton structure in modern human body anatomy. It was viewed that" Shi"recorded in" Shi, Ye, Sui" implied the bone trabecula and a part of compact bone structure. In summary,the paper aims to draw the attention of the field of traditional Chinese medicine to the human anatomy and physiology of traditional Chinese medicine.

  5. Study on Sedative And Hypnotic Effect of LONG GU and CI SHI%龙骨、磁石对小鼠镇静催眠作用的研究

    Institute of Scientific and Technical Information of China (English)

    李光华; 周旭; 贺弋

    2001-01-01

    目的:研究龙骨、磁石水煎剂对小鼠镇静催眠作用,并 对两者进行比较。方法:采用行为药理学方法观察并测定龙骨、磁石对小 鼠最大耐受量,对小鼠自发活动及对阈下剂量和阈剂量戊巴比妥钠催眠作用的影响。结果:ip龙骨、磁石水煎剂200g/kg小鼠最大耐受量倍数为667,龙骨、磁石水煎 剂50、25g/kg等均可显著减少小鼠自发活动,能明显增加阈下剂量戊巴比妥纳小鼠的入睡率 , 可显著缩短戊巴比妥钠小鼠的入睡时间并能延长其睡眠时间。结论:1)龙 骨、磁石均对小鼠具有镇静催眠的作用;2)同浓度时龙骨的镇静催眠作用强于磁石。%Objective:To study and compare the sedative and hypnot ic effect of Long Gu and Ci Shi on mice.Methods:By calculating maxim al tolerance,by observing the changes of spontaneous activity,by measuring the c hange of hypnotic action induced with pentobarbital in subthreshold or threshold doses,effects of Long Gu and Ci Shi were estimated in mice.Results: Being given intraperitoneally Long Gu and Ci shi 200g/kg,the maximal tolerance o f mice was 667 times larger than that of human being.Compared with control gr oup,Long Gu and Ci Shi(50g/kg,25g/kg ip) could remarkedly decrease the spontaneo us motion,enhance the rate of falling asleep induced by sodium pentobabital(in s ubthreshold dose),reduce the latent period of falling asleep,and prolong the sle ep time induced by sodium pentobabital(in suprathreshold dose).Conclusion: Long Gu and Ci Shi possess obviously sedative and hypnotic effects in mi ce,and these effects of Long Gu show somewhat stronger than that of Ci Shi.

  6. Modelo de gestión cultural: Una experiencia con el Qhapaq Ñan en Huánuco

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    Denesy Palacios Jiménez

    2014-06-01

    Full Text Available Objetivos: Plantear el aprovechamiento del patrimonio cultural arqueológico del Qhapaq Ñan en Huánuco en condición de parque cultural, mediante un modelo eficiente de gestión cultural basada en la investigación, protección, conservación y uso social, para convertir a este lugar en un destino turístico y así contribuir con un proyecto no solo para salvaguardarlo sino también para incrementar los ingresos económicos y reducir los niveles de pobreza en la zona. Métodos: Se trata de un estudio que describe y recoge experiencias de gestión cultural respecto a cómo se aprovecha en otros sitios el legado o patrimonio cultural arqueológico, tanto en el ámbito mundial como nacional. La investigación fue cuantitativa y cualitativa, con un alcance correlacional. Se usó el diseño descriptivo, comparativo, analítico, histórico, deductivo. Resultados: El estudio plantea la necesidad de optimizar los recursos para el aprovechamiento del Qhapaq Ñan como parque cultural a través de una adecuada gestión cultural, y que dicho parque comprenderá 68,34 km, desde el tambo de Tambococha o Tunsucancha hasta el tambo de Taparako, por ser uno de los tramos mejor conservados e involucra importantes zonas de patrimonio cultural material e inmaterial. Conclusiones: El Camino Inca en Huánuco es uno de los patrimonios más importantes del país, y es posible ser impulsado como un parque cultural mediante un modelo de gestión cultural, si tenemos en cuenta las diversas experiencias de gestión en el país y en mundo, cuyos aportes deben servir de apoyo para un modelo de gestión en este campo.

  7. Dai Ming Shi and cultural policy of early qing dynasty%戴名世与清前期文化政策

    Institute of Scientific and Technical Information of China (English)

    盛险峰

    2015-01-01

    Because of the Case 'The volume of Nanshan', Dai ming-shi lifetime was full of the pathetic.In fact, exploring his font, articles, and friends: As a representative of scholars, The necessity of the case is because of the identity question between the court and himself , and the phenomenon which qing dynast could' t acceptable.To deal with the Case, Emperor Kangxi took the methods of control and molification, the methods can' t violation the whole cultural policy of 'JI GU YOU WEN' .After this case ended, the scholars of qing dynast, especially for the the place of TongCheng origin, Adjust to the relationships with the reign in time, dispelling the cultural gulf differents outlooks on YI -XIA, and the scholars demostrated the real functions of the ancient Chi-nese prose about the emphasizing statecraft, It not only help the majority of the scholars cut of the relations with previous dynasty, but also encourged the school of Tongcheng established, and then in Qing dynasty 'the powerful of the north and the south' have colli-sioned to expressed the cultural characteristics of summarized and arranged.Dai ming -shi, the watershelds person in place of TongCheng origin which used ancient Chinese prose to make a relationships with the reign .Obviously, As we studying the TongCheng school, we can' t bypassing the person called 'Dai Ming Shi' , more cannot igore the cultural policy in earlier Qing dynasty.%戴名世因 《南山集》 案彰显悲情的一生. 其实, 探究戴名世的字号、 文章及交游发现: 此案发生的必然性在于, 戴氏作为士人的一个代表存在着对朝廷的认同问题, 而这在清统治者看来是不可容忍的. 康熙帝处理此案采取了高压与怀柔并用的手段, 这并不违背 "稽古右文" 以集大成的文化政策. 历经此案劫后, 清代士人群体特别是桐城籍士人, 及时调整了与当朝的关系, 消解夷夏之别的文化隔阂, 彰显古文经世致用的现实功能, 不仅使大部分

  8. Hepatitis B Virus X Upregulates HuR Protein Level to Stabilize HER2 Expression in Hepatocellular Carcinoma Cells

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    Chao-Ming Hung

    2014-01-01

    Full Text Available Hepatitis B virus- (HBV- associated hepatocellular carcinoma (HCC is the most common type of liver cancer. However, the underlying mechanism of HCC tumorigenesis is very complicated and HBV-encoded X protein (HBx has been reported to play the most important role in this process. Activation of downstream signal pathways of epidermal growth factor receptor (EGFR family is known to mediate HBx-dependent HCC tumor progression. Interestingly, HER2 (also known as ErbB2/Neu/EGFR2 is frequently overexpressed in HBx-expressing HCC patients and is associated with their poor prognosis. However, it remains unclear whether and how HBx regulates HER2 expression. In this study, our data showed that HBx expression increased HER2 protein level via enhancing its mRNA stability. The induction of RNA-binding protein HuR expression by HBx mediated the HER2 mRNA stabilization. Finally, the upregulated HER2 expression promoted the migration ability of HBx-expressing HCC cells. These findings deciphered the molecular mechanism of HBx-mediated HER2 upregulation in HBV-associated HCC.

  9. New Light-Travel Time Models and Orbital Stability Study of the Proposed Planetary System HU Aquarii

    CERN Document Server

    Hinse, Tobias C; Gozdziewski, Krzysztof; Haghighipour, Nader; Lee, Chung-Uk; Scullion, Eamon M

    2011-01-01

    In this work we propose a new orbital architecture for the two proposed circumbinary planets around the polar eclipsing binary HU Aquarii. We base the new two-planet, light-travel time model on the result of a Monte Carlo simulation driving a least-squares Levenberg-Marquardt minimisation algorithm on the observed eclipse egress times. Our best-fitting model with $\\chi_{r}^2=1.43$ resulted in high final eccentricities for the two companions leading to an unstable orbital configuration. From a large ensemble of initial guesses we examined the distribution of final eccentricities and semi-major axes for different $\\chi_{r}^2$ parameter intervals and encountered qualitatively a second population of best-fitting parameters. The main characteristic of this population is described by low-eccentric orbits favouring long-term orbital stability of the system. We present our best-fitting model candidate for the proposed two-planet system and demonstrate orbital stability over one million years using numerical integrati...

  10. Characterization and hepatoprotective effect of polysaccharides from Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou sarcocarp.

    Science.gov (United States)

    Yue, Yuan; Wu, Shuangchan; Zhang, Huifang; Zhang, Xuyan; Niu, Yunhui; Cao, Xiaoqin; Huang, Fuwei; Ding, Hong

    2014-12-01

    The polysaccharides from Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou sarcocarp (PWJS) is evaluated for the chemical composition, antioxidant activity and hepatoprotective effect. The characteristics of PWJS were determined by FT-IR spectral and HPLC analysis. The antioxidant activity was investigated using in vitro systems. An in vivo study of PWJS against CCl4 induced liver injury was also conducted. HPLC analysis showed that PWJS is an acidic heteropolysaccharide, rich in glucose (38.59%), arabinose (23.16%), galacturonic acid (17.64%) and galactose (10.44%). PWJS displayed strong antioxidant activity in vitro. In the in vivo study, PWJS treatment lowered the serum levels of ALT and AST in CCl4-intoxicated mice. Additionally, levels of antioxidant enzymes (SOD and CAT) and GSH were elevated in liver damage mice by PWJS intervention, while content of MDA was lessened. Meanwhile, PWJS reverses the suppression of Nrf2 nuclear translocation, and increases the protein expression of HO-1, GSTα and NQO1 in liver damage mice. Hematoxylin-eosin staining showed that the condition of liver damage was mitigated. This study demonstrates that PWJS reverses hepatotoxicity in CCl4-intoxicated mice through the mechanisms of antioxidant activity, as well as an augmentation of the Nrf2 pathway in liver tissue.

  11. A Network Pharmacology Approach to Uncover the Pharmacological Mechanism of XuanHuSuo Powder on Osteoarthritis

    Science.gov (United States)

    Zhang, Xinyue; Luo, Shilin; Zhang, Baixia; Duan, Xiaojie; Zhang, Zhiqian; Wang, Wenqi; Wang, Yun; Sun, Yikun

    2016-01-01

    As the most familiar type of arthritis and a chronic illness of the joints, Osteoarthritis (OA) affects a great number of people on the global scale. XuanHuSuo powder (XHSP), a conventional herbal formula from China, has been extensively applied in OA treatment. Nonetheless, its pharmacological mechanism has not been completely expounded. In this research, a network pharmacology approach has been chosen to study the pharmacological mechanism of XHSP on OA, and the pharmacology networks were established based on the relationship between four herbs found in XHSP, compound targets, and OA targets. The pathway enrichment analysis revealed that the significant bioprocess networks of XHSP on OA were regulation of inflammation, interleukin-1β (IL-1β) production and nitric oxide (NO) biosynthetic process, response to cytokine or estrogen stimuli, and antiapoptosis. These effects have not been reported previously. The comprehensive network pharmacology approach developed by our research has revealed, for the first time, a connection between four herbs found in XHSP, corresponding compound targets, and OA pathway systems that are conducive to expanding the clinical application of XHSP. The proposed network pharmacology approach could be a promising complementary method by which researchers might better evaluate multitarget or multicomponent drugs on a systematic level. PMID:27110264

  12. Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

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    Chun-Ching Lin

    2012-01-01

    Full Text Available Xiao-Chai-Hu-Tang (XCHT, a traditional Chinese medicine formula consisting of seven medicinal plants, is used in the treatment of various diseases. We show here that XCHT could protect type-1 diabetic mice against diabetic nephropathy, using streptozotocin (STZ-induced diabetic mice and high-glucose (HG-exposed rat mesangial cell (RMC as models. Following 4 weeks of oral administration with XCHT, renal functions and renal hypertrophy significantly improved in the STZ-diabetic mice, while serum glucose was only moderately reduced compared to vehicle treatment. Treatment with XCHT in the STZ-diabetic mice and HG-exposed RMC resulted in a decrease in expression levels of TGF-β1, fibronectin, and collagen IV, with concomitant increase in BMP-7 expression. Data from DPPH assay, DHE stain, and CM-H2DCFDA analysis indicated that XCHT could scavenge free radicals and inhibit high-glucose-induced ROS in RMCs. Taken together, these results suggest that treatment with XCHT can improve renal functions in STZ-diabetic mice, an effect that is potentially mediated through decreasing oxidative stress and production of TGF-β1, fibronectin, and collagen IV in the kidney during development of diabetic nephropathy. XCHT, therefore merits further investigation for application to improve renal functions in diabetic disorders.

  13. Synergistic Anticancer Effects of Vorinostat and Epigallocatechin-3-Gallate against HuCC-T1 Human Cholangiocarcinoma Cells

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    Tae Won Kwak

    2013-01-01

    Full Text Available The aim of this study was to investigate the effect of the combination of vorinostat and epigallocatechin-3-gallate against HuCC-T1 human cholangiocarcinoma cells. A novel chemotherapy strategy is required as cholangiocarcinomas rarely respond to conventional chemotherapeutic agents. Both vorinostat and EGCG induce apoptosis and suppress invasion, migration, and angiogenesis of tumor cells. The combination of vorinostat and EGCG showed synergistic growth inhibitory effects and induced apoptosis in tumor cells. The Bax/Bcl-2 expression ratio and caspase-3 and -7 activity increased, but poly (ADP-ribose polymerase expression decreased when compared to treatment with each agent alone. Furthermore, invasion, matrix metalloproteinase (MMP expression, and migration of tumor cells decreased following treatment with the vorinostat and EGCG combination compared to those of vorinostat or EGCG alone. Tube length and junction number of human umbilical vein endothelial cells (HUVECs decreased as well as vascular endothelial growth factor expression following vorinostat and EGCG combined treatment. These results indicate that the combination of vorinostat and EGCG had a synergistic effect on inhibiting tumor cell angiogenesis potential. We suggest that the combination of vorinostat and EGCG is a novel option for cholangiocarcinoma chemotherapy.

  14. Trigeminal Neuralgia as the First Clinical Manifestation of Anti-Hu Paraneoplastic Syndrome Induced by a Borderline Ovarian Mucinous Tumor

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    Hossein Kalanie

    2014-01-01

    Full Text Available Paraneoplastic neurologic syndrome (PNS is an uncommon manifestation of cancer that is not caused by the tumor or metastasis. Trigeminal neuralgia (TN is an initial symptom of this disease, but it has rarely been reported in the literature. Here, we report the case of a 76-year-old woman who presented with classic TN, followed by limbic encephalitis due to an underlying ovarian intestinal-type mucinous borderline tumor, with the presence of anti-Hu antibodies. She recovered quickly after removal of the tumor and was essentially free of symptoms 2 weeks after surgery. Because PNS precedes the tumor in approximately 60% of cases, its rapid detection and treatment are crucial. Therefore, we propose that PNS be considered during the management of TN when brain imaging is normal, as it is followed by other central and/or peripheral neurological manifestations as well as the presence of systemic symptoms such as anemia, fatigability, loss of appetite, or weight loss.

  15. Bioactive comparison of main components from unripe fruits of Rubus chingii Hu and identification of the effective component.

    Science.gov (United States)

    Zhang, Tian-Tian; Yang, Li; Jiang, Jian-Guo

    2015-07-01

    Dried and unripe fruit of Rubus chingii Hu, known as "Fu-pen-zi" in Chinese, has been used as a food and tonic in China for a long time. In order to analyze its effective ingredients, polysaccharides, flavonoids, saponins and alkaloids were extracted from the unripe fruits and their contents were determined. The in vitro antioxidant, anticomplementary and anticancer activities against human lung adenocarcinoma A549 cells of the four major constituents were investigated. Results showed that total flavonoids exhibited an obvious antioxidant activity, which was very close to ascorbic acid. The anticomplementary and anticancer activities of flavonoids were also the best among the four chemical compositions. Therefore, extraction process optimization of flavonoids was conducted using response surface methodology. The optimal conditions were as follows: extraction temperature 72.8 °C, ethanol concentration 30.67%, extraction time 2.66 h, and a liquid/solid ratio of 19.54 : 1. In addition, total flavonoids were subsequently separated by column chromatography and the major flavonoid was identified as tiliroside. Further experimental data revealed that tiliroside treatment could suppress the proliferation and induced the apoptosis of A549 cells.

  16. 胡绳史料学思想初探%Preliminary Study on Hu Sheng' s Thoughts of Historical Materials

    Institute of Scientific and Technical Information of China (English)

    胡现岭

    2012-01-01

    Hu Sheng was critical to the historians who were good at historical materials,arguing that their work was only the initial stage of historical research.This attitude didn' t mean that he ignored the important role of historical materials.He pointed out that we should guide historical materials by Marxism and historical data should service views;In addition,when carrying out the work of historical materials,we should estimate its value at the same time.%胡绳对以史料见长的史家一贯持批评态度,认为他们的工作只是停留在史学研究的初始阶段,但这并不表明他不重视史料在史学研究中的作用,而是提出以马克思主义来指导史料工作,在对史料进行考证的同时对其价值的高低做出评判,强调史料要为观点服务。

  17. Karyotypes of Akodon orophilus Osgood 1913 and Thomasomys sp. (Rodentia: Sigmodontinae from Huánuco, Peru

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    Víctor Pacheco

    2012-10-01

    Full Text Available Conventional chromosomal preparations were made of three native mice from Huánuco, Peru: a male and a female of Thomasomys sp., and a male of Akodon orophilus. Thomasomys sp. had a karyotype of 2n = 42, XY (n = 21, meanwhile A. orophilus presented 2n = 22, XY (n = 11. Comparisons between chromosomal pairs from the existent literature indicate that both are new karyotypes. Thomasomys sp. has a distinct sexual Y chromosome, the only metacentric (m reported for the genus. The chromosomes X and Y of A. orophilus are acrocentrics (a; and the length of chromosome Y (2/3 of the length of X distinguishes A. orophilus from other congeneric. Because the structural differences between the sexual chromosomes usually generates mechanism of reproductive isolation at intraspecific level and are bigger still in interspecific crosses, we concluded that the karyotypes reported here support the validity of the species A. orophilus and suggest that Thomasomys sp. represents a new species to science.

  18. Optimization of in vitro HBV replication and HBsAg production in HuH7 cell line.

    Science.gov (United States)

    Cavallone, Daniela; Moriconi, Francesco; Colombatto, Piero; Oliveri, Filippo; Bonino, Ferruccio; Brunetto, Maurizia Rossana

    2013-04-01

    The Gunther's vector-free method (GM), using PCR-amplified full length HBV-DNA (fl-HBV-DNA), is currently the best in vitro HBV replication system despite the low intracellular HBV-DNA production. The replication efficiency and HBsAg secretion of 12 isolates from HBsAg/HBeAg positive sera by GM, Monomer-Linear-Sticky-Ends-DNA (MLSE) and Monomer-Circular-Closed (MCC) were compared in HuH7 cells. Eight of twelve genomes (67%) were replication competent by GM; however direct sequencing (DS) showed that more than 80% of input DNA was undigested in spite of SapI treatment. Replication Intermediates (RI) were detected earlier (24 vs. 48h) and in higher amounts (2.51±0.32 and 6.43±0.43 fold) by MCC than GM or MLSE. By MCC 10 of 12 genomes (83%) were replication competent and 7 produced high RI levels. RI and HBsAg kinetics correlated positively in MCC (R=0.696, p=0.017 overall; R=0.928, p=0.008), but not in GM (R=-0.437, p=0.179 overall; R=-0.395, p=0.439) in genotype D isolates. In conclusion, HBV-DNA circularization prior transfection improves in vitro viral replication and replication competent HBsAg production, mimicking better the in vivo conditions.

  19. 蛙式扳法治疗骶髂关节源性下腰痛的临床疗效观察%Clinical Observation on Curative Effect of Wa-shi Pulling on Treating Sacroiliac Joint Pain

    Institute of Scientific and Technical Information of China (English)

    诸波; 范炳华; 王鹏; 应晓明; 徐泉珍; 郭少卿

    2012-01-01

    Objective: To observe the clinical efficacy of the Wa - shi pulling on treating sacroiliac joint pain and to explore the mechanism of the Wa - shi pulling. Methods: 60 patients diagnosed as sacroiliac joint pain were collected and treated with the four operative steps of the Wa - shi pulling to observe the clinical efficacy. Results: The treatment time ranged from 1 to 7 (average 4 times). According to evaluation standard of sacroiliac joint pain, the results were cured in 50 cases,improvement in 9 cases,inefficacy in 1 case. Conclusion:The Wa - shi pulling had many advantages such as targeted and quick effect and high rate of success in treatment of sacroiliac joint pathological changes due to low back pain.%目的:通过观察蛙式四步扳法治疗骶髂关节源性下腰痛的临床疗效,探讨蛙式四步扳法的作用机制.方法:临床收集被确诊为骶髂关节源性下腰痛患者60例,采用自体牵引,极度屈髋屈膝,蛙式扳法和后伸扳法四步操作,观察其疗效.结果:治疗次数1~7次,平均治疗4.0次.疗效评价治愈50例,好转9例,未愈1例.结论:蛙式四步扳法治疗骶髂关节源性下腰痛针对性强,见效快,成功率高.

  20. 塞隆风湿酒对小鼠镇痛、抗寒的实验研究%Experimental Research Analgesic and resist-cool effects in Mice by Sai long Feng shi Wine

    Institute of Scientific and Technical Information of China (English)

    徐淑玲; 沈华; 王笑红

    2001-01-01

    To investigate Analgesic and resist-cool effects of sai long Feng shi wine in mice. Methods: Analgesic action of sai long Feng shi wine was obserated acetic acid-induced writhing test in mice. Anti-cool test was obserad in mice in -20℃ refrigerator.Results: Sai long feng shi wine can inhibit writing reaction and prolong the surrival time in cooled mice.%目的:观察塞隆风湿酒A、B及大小剂量对小鼠镇痛、抗寒冷作用影响。方法:采用昆明系小鼠灌胃塞隆酒A、B不同剂量,观察各组动物对醋酸致痛后发生扭体反应数及各组动物在-20℃低温条件下存活时间。结果:塞隆风湿酒A、B大小剂量对实验动物具有明显的镇痛及抗寒作用。

  1. Avaliação dos óbitos ocorridos no Hospital Universitário da Universidade Federal de Santa Catarina (HU/UFSC Evaluation of deaths occurred at the University Hospital (HU/UFSC

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    Rachel Duarte Moritz

    2008-10-01

    Full Text Available OBJETIVO: Avaliar os óbitos ocorridos no HU/UFSC. Comparar o perfil dos pacientes que morreram nas enfermarias com o daqueles que morreram na UTI. Verificar se os óbitos foram considerados esperados (E e não evitáveis (NE. Constatar se houve recusa/suspensão de terapêutica (RST precedendo a morte. MÉTODOS: Obtivemos dados por meio de ficha elaborada pela Comissão de Óbito Hospitalar (COH, na qual constavam os aspectos demográficos, clínicos e terapêuticos dos pacientes e a avaliação da COH sobre a expectativa/evitabilidade dos óbitos. RESULTADOS: Analisamos os dados dos 326 pacientes com mais de 14 anos, que morreram entre julho/2004 e dezembro/2005. Desses, 128 morreram na UTI (G1 e 198 nas enfermarias (G2. A RST precedeu 38,2% dos óbitos no G1 e 2% no G2 (pOBJECTIVE: To evaluate the deaths which occurred at the HU/UFSC. To compare the profile of patients who died in the wards with that of patients who died in the ICU. To classify deaths which were expected or not, and avoidable or not. To verify how often withholding or withdrawing (WW therapy preceded death. METHODS: Archives of the Hospital Death Commission (HDC were analyzed. The clinical and demographic records were retrieved from the HDC data bank. Deaths were classified by the HDC as expected or not and avoidable or not. RESULTS: Data from 326 dead persons over 14 years of age were analyzed. One hundred and twenty eight deaths occurred in the ICU (G1 and 198 in the wards (G2. WW therapy preceded 38.2% of deaths in G1 and 2% in G2 (p<0.001. The main WW therapies were vasoactive drugs and antibiotics. Almost 20% of deaths were unexpected/avoidable in G1. In G2 6.5% were considered unexpected and 5% avoidable (p<0.005. Patients in G1 were younger and mostly male (p<0.005. Do-not-resuscitate orders were registered in 48.4% of patients' medical charts in G2 and 6.3% in G1 (p<0.001. Cardiopulmonary resuscitation was performed in 23.4% of patients in G1 and in 5.5% in G2 (p<0

  2. 22(R)-hydroxycholesterol induces HuR-dependent MAP kinase phosphatase-1 expression via mGluR5-mediated Ca(2+)/PKCα signaling.

    Science.gov (United States)

    Kim, Hyunmi; Woo, Joo Hong; Lee, Jee Hoon; Joe, Eun-Hye; Jou, Ilo

    2016-08-01

    MAP kinase phosphatase (MKP)-1 plays a pivotal role in controlling MAP kinase (MAPK)-dependent (patho) physiological processes. Although MKP-1 gene expression is tightly regulated at multiple levels, the underlying mechanistic details remain largely unknown. In this study, we demonstrate that MKP-1 expression is regulated at the post-transcriptional level by 22(R)-hydroxycholesterol [22(R)-HC] through a novel mechanism. 22(R)-HC induces Hu antigen R (HuR) phosphorylation, cytoplasmic translocation and binding to MKP-1 mRNA, resulting in stabilization of MKP-1 mRNA. The resulting increase in MKP-1 leads to suppression of JNK-mediated inflammatory responses in brain astrocytes. We further demonstrate that 22(R)-HC-induced phosphorylation of nuclear HuR is mediated by PKCα, which is activated in the cytosol by increases in intracellular Ca(2+) levels mediated by the phospholipase C/inositol 1,4,5-triphosphate receptor (PLC/IP3R) pathway and translocates from cytoplasm to nucleus. In addition, pharmacological interventions reveal that metabotropic glutamate receptor5 (mGluR5) is responsible for the increases in intracellular Ca(2+) that underlie these actions of 22(R)-HC. Collectively, our findings identify a novel anti-inflammatory mechanism of 22(R)-HC, which acts through PKCα-mediated cytoplasmic shuttling of HuR to post-transcriptionally regulate MKP-1 expression. These findings provide an experimental basis for the development of a RNA-targeted therapeutic agent to control MAPK-dependent inflammatory responses.

  3. Intratumoral hu14.18-IL-2 (IC) induces local and systemic antitumor effects that involve both activated T and NK cells as well as enhanced IC retention.

    Science.gov (United States)

    Yang, Richard K; Kalogriopoulos, Nicholas A; Rakhmilevich, Alexander L; Ranheim, Erik A; Seo, Songwon; Kim, Kyungmann; Alderson, Kory L; Gan, Jacek; Reisfeld, Ralph A; Gillies, Stephen D; Hank, Jacquelyn A; Sondel, Paul M

    2012-09-01

    hu14.18-IL-2 (IC) is an immunocytokine consisting of human IL-2 linked to hu14.18 mAb, which recognizes the GD2 disialoganglioside. Phase 2 clinical trials of i.v. hu14.18-IL-2 (i.v.-IC) in neuroblastoma and melanoma are underway and have already demonstrated activity in neuroblastoma. We showed previously that intratumoral hu14.18-IL-2 (IT-IC) results in enhanced antitumor activity in mouse models compared with i.v.-IC. The studies presented in this article were designed to determine the mechanisms involved in this enhanced activity and to support the future clinical testing of intratumoral administration of immunocytokines. Improved survival and inhibition of growth of both local and distant tumors were observed in A/J mice bearing s.c. NXS2 neuroblastomas treated with IT-IC compared with those treated with i.v.-IC or control mice. The local and systemic antitumor effects of IT-IC were inhibited by depletion of NK cells or T cells. IT-IC resulted in increased NKG2D receptors on intratumoral NKG2A/C/E⁺ NKp46⁺ NK cells and NKG2A/C/E⁺ CD8⁺ T cells compared with control mice or mice treated with i.v.-IC. NKG2D levels were augmented more in tumor-infiltrating lymphocytes compared with splenocytes, supporting the localized nature of the intratumoral changes induced by IT-IC treatment. Prolonged retention of IC at the tumor site was seen with IT-IC compared with i.v.-IC. Overall, IT-IC resulted in increased numbers of activated T and NK cells within tumors, better IC retention in the tumor, enhanced inhibition of tumor growth, and improved survival compared with i.v.-IC.

  4. A new species of frog of the genus Pristimantis from Tingo María National Park, Huánuco Department, central Peru (Anura, Craugastoridae)

    Science.gov (United States)

    Chávez, Germán; Catenazzi, Alessandro

    2016-01-01

    Abstract A new species of Craugastoridae frog encountered from 1000–1700 m in elevation in the premontane forests of the Peruvian central Andes is described. The new species is similar in appearance to many other species of Pristimantis, but is easily distinguishable from these species by having bright red coloration on the groin, posterior surface of thighs, and shanks. The new species is only known for two localities 27 km apart in the Huánuco Region. PMID:27587978

  5. Contents of the heavy metals nickel and lead in leaves of Paulownia elongata S.Y. Hu and Paulownia fortunei Hems. in Serbia

    OpenAIRE

    2009-01-01

    A comparative study was carried out on the capabilities of the two species Paulownia elongata S. Y. Hu and Paulownia fortunei Hems. as biomonitors of atmospheric (and soil) heavy-metal pollution. Research on content of the heavy metals nickel (Ni) and lead (Pb) in leaves of the species Paulownia elongata growing under urban and suburban conditions with the comparison to the concentration of these elements in leaves of the species Paulownia elongata and Paulownia fortunei on an experimental pl...

  6. La presencia de Ixodes luciae en el noroeste argentino y nuevos huéspedes para Ixodes pararicinus y algunas especies de Amblyomma (Acari: Ixodidae

    Directory of Open Access Journals (Sweden)

    Analía G. AUTINO

    2006-01-01

    Full Text Available El análisis de una colección de garrapatas de mamíferos del noroeste argentino, depositados en la Colección de Anexos de la Colección Mamíferos Lillo (CML de la Universidad Nacional de Tucumán, permitió ampliar el área de distribución de Ixodes luciae Sénevet, a las provincias de Salta y Tucumán e incrementar su rango de huéspedes al roedor sigmodontino Calomys callosus (Rengger y a los marsupiales de la familia Didelphidae Micoureus constantiae (Thomas, Thylamys cinderella Thomas y Thylamys venustus (Thomas. Se registró a C. callosus y Oligoryzomys destructor (Tschudi como nuevos huéspedes de Ixodes pararicinus Keirans & Clifford, y a los Carnivora Lycalopex gymnocercus (Fischer y Oncifelis geoffroyi (d ́Orbigny & Gervais como nuevos huéspedes de Amblyomma neumanni Ribaga y Amblyomma parvum Aragão, respectivamente. Adicionalmente, se observó la infestación de Lutreolina crassicaudata (Desmarest (Didelphidae por I. luciae y la presencia de Amblyomma tigrinum Koch, sobre L. gymnocercus. Ninfas de Amblyomma sp. se obtuvieron de Akodon Meyen (Sigmodontinae.

  7. Effects of r—HuEPO on the biophysical characteristics of erythrocyte membrane in patients with anemia of chronic renal failure

    Institute of Scientific and Technical Information of China (English)

    SHIHONGLIAN; FAJUNYANG; 等

    1994-01-01

    Using electron spin resonance (ESR) spin labeling technique,we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and mem brane fluidity of red blood cells(RBCs) from two groups of patients with anemia of chronic renal failure(ACRF).One of the groups is composed of patients who were untreated with recombinant human erythropoietin(r-HuEPO),and the other is composed of patients who were treated with r-HuEPO.The results indicated:1)the conformation of SH group binding site in RBC membrane proteins from former group was different from those of healty people.2)the fluidity in the region near the surface of RBC membrane from former group was lower than those of healthy people.3)However,the above biophysical properties of RBC membrane from later group were normal.We concluded that RBC membrane in patients with ACRF was abnormal,and the treatment of r-HuEPO may promote the production of normal RBCs,thus ameliorate the biophysical properties of RBCs from the patients with ACRF.

  8. HuR and Ago2 Bind the Internal Ribosome Entry Site of Enterovirus 71 and Promote Virus Translation and Replication.

    Directory of Open Access Journals (Sweden)

    Jing-Yi Lin

    Full Text Available EV71 (enterovirus 71 RNA contains an internal ribosomal entry site (IRES that directs cap-independent initiation of translation. IRES-dependent translation requires the host's translation initiation factors and IRES-associated trans-acting factors (ITAFs. We reported recently that mRNA decay factor AUF1 is a negative-acting ITAF that binds IRES stem-loop II. We also reported that the small RNA-processing enzyme Dicer produces at least four small RNAs (vsRNAs from the EV71 IRES. One of these, vsRNA1, derived from IRES stem-loop II, reduces IRES activity and virus replication. Since its mechanism of action is unknown, we hypothesized that it might control association of ITAFs with the IRES. Here, we identified the mRNA stability factor HuR and the RISC subunit Argonaute 2 (Ago2 as two ITAFs that bind stem-loop II. In contrast to AUF1, HuR and Ago2 promote EV71 IRES activity and virus replication. In vitro RNA-binding assays revealed that vsRNA1 can alter association of Ago2, HuR, and AUF1 with stem-loop II. This presents a possible mechanism by which vsRNA1 could control viral translation and replication.

  9. 论胡锦涛党建思想的基本价值%On the fundamental value of the Party building thought Hu Jintao

    Institute of Scientific and Technical Information of China (English)

    李强

    2011-01-01

    Since Sixteenth National Congress of the Communist Party of China,Hu Jintao as general secretary of the party leadership to actively promote the construction of the new great project,and achieved fruitful theoretical results,the formation of a building thought Hu.Hu building thought to enrich and develop Marxism in the ideological theory of party building treasury,for the party is always ahead of the times,firmly implement the Party's governing mission to provide a scientific theory and a powerful ideological weapon.%党的十六大以来,以胡锦涛为总书记的领导集体积极推进党的建设的伟大工程,取得了丰硕的理论成果,并形成了胡锦涛党建思想。胡锦涛党建思想丰富和发展了马克思主义党建理论的思想宝库,为使党始终走在时代前列,坚定不移实现党的执政使命,提供了科学理论和强大的思想武器。

  10. A comparative study of oral health attitudes and behavior using the Hiroshima University-Dental Behavioral Inventory (HU-DBI) between dental and civil engineering students in Colombia.

    Science.gov (United States)

    Jaramillo, Jorge A; Jaramillo, Fredy; Kador, Itzjak; Masuoka, David; Tong, Liyue; Ahn, Chul; Komabayashi, Takashi

    2013-03-01

    The aim of this study was to use the Hiroshima University - Dental Behavioral Inventory (HU-DBI) to compare oral health attitudes and behavior of dental and civil engineering students in Colombia. The HU-DBI's survey consisting of twenty dichotomous responses (agree-disagree) regarding tooth brushing, was completed at University Antonio Narino for the dental students and the University of Cauca for the civil engineering students. The Spanish version of the HU-DBI questionnaire was taken by 182 of 247 dental students and 411 of 762 engineering students. The data was-statistically analyzed by the chi-square test and backward logistic regression. Compared to the engineering students, the dental students were more likely to agree with questions such as "I am bothered by the color of my gums"(OR = 2.2, 95% CI: 1.3-3.7),"I think I can clean my teeth well without using toothpaste" (OR = 3.0, 95% CI: 1.5-5.9), "I have used a dye to see how clean my teeth are" (OR = 2.9, 95% CI: 1.9-4.3), and "I have had my dentist tell me that I brush very well" (OR = 2.0, 95% CI: 1.3-3.1). The dental education curriculum in a dental school compared to a civil engineering school in Colombia indicated that a three-phase curriculum in didactics and clinics increased oral health attitudes and behavior from entry to graduation.

  11. [Textual research on Guang dong xin yu (New Sayings of Guangdong) quoted in Ben cao gang mu shi yi (Supplements to Compendium of Materia Medica].

    Science.gov (United States)

    Zhang, Ruixian; Zhang, Wei; Li, Jian; Liang, Fei

    2014-05-01

    Altogether 15 terms for Guang dong xin yu (New Sayings of Guangdong) were used in Ben cao gang mu shi yi (Supplements to Compendium of Materia Medica), including Yue yu (Cantonese sayings), Chong yu (Sayings from Insect Drug), Jie yu (Sayings from Crustacean Drug), Xin yu (New Sayings), Yue hai xiang yu (Fragrant Sayings from Cantonese Region), Yue zhi mu yu (Sayings from Plants in Cantonese Annals), Guang dong suo yu (Trivial Sayings from Guangdong), Yue shan lu (Records of Cantonese Mountains), Yue lu (Cantonese Records), Jiao guang lu (Joint Guangdong Records), Yue cao zhi (Records of Cantonese Grasses), Guang guo lu (Records of Guangdong Fruits), Nan yue suo ji (Trivial Records of Southern Canton), Guang zhi (Guangdong Records), Yue zhi (Cantonese Records) etc. dealing with 57 sorts of drugs (with individual overlapping ones), the author of Xin yu was Qu Dajun, a surviving fogy of the Ming Dynasty actively involved in the activities to restore the old dynasty and resist the Qing Dynasty, and was persecuted in the literary inquisition in which his works were burnt so that Zhao Xuemin, when quoting his texts, had to go in a roundabout way.

  12. 秦始皇陵园K9901试掘简报%Trial Excavation of the Unit K9901 at the Mausoleum Garden of Qin Shi Huangdi

    Institute of Scientific and Technical Information of China (English)

    始皇陵考古队

    2001-01-01

    The accompanying burial pit K9901 is situated in the southeast of the territory within the outer walls of the Qin Shi Huangdi mausoleum garden and occupies an area of 700 sq m. A trial excavation in a part of it was carried out by the Qin Shl Huangdi Mausoleum Archaeological Team in May—June 1999. The pit is a typical earthen-and-wooden gallery-shaped underground structure, the wooden part of which was destroyed by fire shortly after the completion of the building. The astonishing finds from the trial excavation are a huge bronze ding and eleven terra-cotta figures of acrobats. The ding is a double-eared tripod with exquisite designs on the ears, belly and leg-bases, weighs 212kg and must have been an important ritual vessel in the ancestral temple of the royal family. The terra-cotta acrobats are yielded from the third passage pit. Dressed in short skirts and being in various postures, they represent different acrobatic performances and provide valuable material for studying entertainment culture in the Qin palace and the history of Chinese acrobatics.

  13. A Study on Jiao Shi Yi Lin’ s Gods Faith%从《焦氏易林》看汉代的神灵信仰

    Institute of Scientific and Technical Information of China (English)

    刘志平

    2015-01-01

    As a special cultural classic written roughly in the period of the mid-late Western Han Dynasty and the early Eastern Han Dynasty,Jiao Shi Yi Lin contains richly historical and cultural information. Record about Han people’ s faith to gods,such as the God Sun, the God Moon, the God Wind, the God Rain, the God Land and the God Mountain, shows us their complex gods faith world, and makes us understand their spirit world and knowledge system and their behaviors in daily life affected by this kind of spirit world and knowledge system more minutely and deeply.%《焦氏易林》作为一部大致成书于西汉中晚期至东汉初期的特殊文化典籍,蕴含着丰富的历史文化信息。其中关于日神、月神、风神、雨神、社神和山神崇拜的内容向我们展现了汉代人复杂的神灵信仰世界,使我们更细致、更深入地了解时人的精神世界和知识系统,以及时人在这种精神世界和知识系统影响下的日常生活行为。

  14. 成都老中医史伟治疗阳痿临床经验%Clinical experience of TCM master Shi Wei for treating impotence

    Institute of Scientific and Technical Information of China (English)

    史伟

    2015-01-01

    Objective: To summarize the experience for treating impotence treatment by TCM master Shi Wei. Methods:Application of psychological counseling, combined with TCM decoction with soft powder, tonic conditioning, diet supplement physical treatment method, change the way of life, according to the etiology, treatment. Conclusion: The clinical verification, the comprehensive TCM method, clinical accurately detailed examination of etiology, the polygenetic, etiology will be very helpful.%目的:总结老中医史伟治疗阳痿的治疗经验。方法:应用心理疏导,中药汤剂,配合粉剂冲服,膏滋补剂调理,饮食补充体质,改变生活方式,采用五位一体的治疗方法,然后依据病因分步治疗。结论:临床证实,应用老中医独创的五位一体分步治疗方法,临床准确审查病因,把握一病多因,审因论治,能取得满意的临床效果。

  15. 刘知几《史通·论赞》考析%Analysis of Shi Tong·Comments by Liu Zhiji

    Institute of Scientific and Technical Information of China (English)

    尹静静

    2012-01-01

    Comment,a form adopted to discuss history in ancient China,is to present an author's or commentator's viewpoints on historical figures and the evaluation of historical events.Centered on Liu Zhiji's monograph Shi Tong·Comments and starting from his definition of comment,the present paper focuses on the four aspects of his comments.Then it explores his standpoints on writing history books embodied in the monograph.The paper ultimately analyzes what is to be desired of the monograph from the perspectives of "what is said by Sima Qian" and the source of comments.%该文以《史通.论赞》篇为中心,从刘知几对"论赞"的命名入手,集中分析了其论赞观的四个方面,进而探讨该篇中所体现的刘知几的著史思想,最后又从"太史公曰"及论赞源流的角度对篇目中值得商榷之处予以辨析。

  16. 浅谈唯识学的梦境言说——以《唯识二十颂》为文本%Topic A Brief Probe into Dream-Logos of the Mind-Only School --Taking "Wei-Shi-Er-Shi-Song" as Study Case

    Institute of Scientific and Technical Information of China (English)

    陈昊

    2011-01-01

    佛教最终目标指向个人开悟与众生解脱,因而有种种言说法门,如说人生如梦、梦境非实有,则不必执迷此一期生命因缘际会。尤其是唯识学,主张“万法唯识”,认为外境俱非实有,皆为阿赖耶识所变现,于是,“做梦”的譬喻就很贴切地表达了唯识学的内涵,也恰到好处地回应了外道小乘对唯识学的责难。分析《唯识二十颂》中做梦、关于梦境的责难与辩说,可窥唯识学理论展开向路之一二。%As the ultimate aim of Buddhism point to personal enlightenment and liberation for all living creatures, there have various Logos, as said, life is like a dream. Dream are not exaiting, then there is no need to obsession with the life's karma.Eapecially the theory of Mind-Only School, advocated "the ten thousand dharmas are only consciousness' creation",and considered all the things outside consciousness, the realization of Alayavijnana , are unre al-exsiting.Therefore,dream as Metaphor aptly expressed the theory's oonnotation,also be just perfect response to other clans and Hinayana's criticizes. Analysis the part of Dream-Logos in "Wei-Shi-Er-Shi-Song",Blame and argue about dreams,Can see some Expansion of theoretical direction of the Mind-Only shehool.

  17. ESR1 Gene Polymorphisms and Prostate Cancer Risk: A HuGE Review and Meta-Analysis.

    Directory of Open Access Journals (Sweden)

    Yu-Mei Wang

    Full Text Available Many published data on the association between single nucleotide polymorphisms (SNPs in the ESR1 gene and prostate cancer susceptibility are inconclusive. The aim of this Human Genome Epidemiology (HuGE review and meta-analysis is to derive a more precise estimation of this relationship.A literature search of PubMed, Embase, Web of Science and Chinese Biomedical (CBM databases was conducted from their inception through July 1st, 2012. Crude odds ratios (ORs with 95% confidence intervals (CIs were calculated to assess the strength of association.Twelve case-control studies were included with a total 2,165 prostate cancer cases and 3,361 healthy controls. When all the eligible studies were pooled into the meta-analysis, ESR1 PvuII (C>T and XbaI (A>G polymorphisms showed no association with the risk of prostate cancer. However, in the stratified analyses based on ethnicity and country, the results indicated that ESR1 PvuII (C>T polymorphism was significantly associated with increased risk of prostate cancer among Asian populations, especially among Indian population; while ESR1 XbaI (A>G polymorphism may significantly increase the risk of prostate cancer among American population. Furthermore, we also performed a pooled analysis for all eligible case-control studies to explore the role of codon 10 (T>C, codon 325 (C>G, codon 594 (G>A and +261G>C polymorphisms in prostate cancer risk. Nevertheless, no significant associations between these polymorphisms and the risk of prostate cancer were observed.Results from the current meta-analysis indicate that ESR1 PvuII (C>T polymorphism may be a risk factor for prostate cancer among Asian populations, especially among Indian population; while ESR1 XbaI (A>G polymorphism may increase the risk of prostate cancer among American population.

  18. CYP3A4*1B polymorphism and cancer risk: a HuGE review and meta-analysis.

    Science.gov (United States)

    Zhou, Li-Ping; Yao, Fan; Luan, Hong; Wang, Yin-Ling; Dong, Xi-Hua; Zhou, Wen-Wen; Wang, Qi-Hui

    2013-04-01

    CYP450 3A4 (CYP3A4), encoded by the CYP3A4 gene, is a major enzyme catalyzing the metabolism of both endogenous and exogenous agents that may play a role in the etiology of carcinogenesis. Several potentially functional polymorphisms of the CYP3A4 gene have been implicated in cancer risk, but individually published studies have shown inconclusive results. The aim of this Human Genome Epidemiology (HuGE) review and meta-analysis was to investigate the association between CYP3A4*1B (rs2740574 A > G) polymorphism and cancer risk. Eleven studies were included with a total of 3,810 cancer patients and 3,173 healthy controls. We found that the G allele and GG genotype of CYP3A4*1B polymorphism were associated with increased risk of cancers using the fixed effects model (allele model: odds ratio (OR) = 1.24, 95 %CI: 1.09-1.42, P = 0.001; recessive model: OR = 1.77, 95 %CI: 1.30-2.41, P cancer type showed that the G allele and G carrier (AG + GG) of CYP3A4*1B polymorphism had significant associations with increased risk of prostate cancer, but not with breast cancer, leukemia, or other cancers. With further subgroup analysis based on different ethnicities, the results indicated that the GG genotype of CYP3A4*1B polymorphism might increase the risk of cancer among African populations. However, similar associations were not observed among Caucasian and Asian populations. Results from the current meta-analysis indicate that the G allele and GG genotype of CYP3A4*1B polymorphism might be associated with increased cancer risk, especially for prostate cancer among African populations.

  19. Bai-hu-tang, ancient chinese medicine formula, may provide a new complementary treatment option for sepsis.

    Science.gov (United States)

    Lin, Chien-Jung; Su, Yi-Chang; Lee, Cheng-Hung; Li, Tsai-Chung; Chen, Yun-An; Lin, Sunny Jui-Shan

    2013-01-01

    Bai-Hu-Tang (BHT) has been broadly applied to treating the early stage of acute infection with systemic inflammation for two thousand years in Chinese medicine. We explore whether BHT is beneficial in treating sepsis and its effects on proinflammatory cytokine, interleukin-6, and anti-inflammatory cytokine interleukin-10, in which both play key roles in the progress of sepsis. Thirty-six male Sprague-Dawley rats were randomized into six groups, with cecal ligation and puncture (CLP) performed in all but the sham-control group. Rats in CLP + BHT-L6 and CLP + BHT-H6 groups, respectively, received a low (0.45 g/kg) and high doses (0.9 g/kg) of BHT, 6 hrs postoperatively. CLP + BHT-L12 and CLP + BHT-H12 groups, respectively, received low and high doses of BHT, 12 hrs postoperatively. Sham-control and sepsis-control groups received distilled water (1 mL) as vehicle, 6 hrs postoperatively. Serial blood samples were drawn before operation, as baseline, and at 4, 8, and 12 hrs postoperatively for IL-6 and IL-10 assay. All rats were monitored for 3 days for survival study. Rats in the CLP + BHT-H6 group had significantly higher survival rate (80%) and significantly lower levels of both IL-6 and IL-10 at 12 hrs postoperatively than those in the sepsis-control group. Results suggested that BHT may be a new complementary treatment option for sepsis.

  20. Redescription and molecular analysis of Myxobolus shantungensis Hu, 1965 (Myxozoa: Myxosporea) infecting common carp Cyprinus carpio haematopterus.

    Science.gov (United States)

    Liu, Yang; Gu, Zemao; Zhang, Yunchao; Zeng, Lingbing

    2011-12-01

    Myxobolus shantungensis Hu, 1965 infects gill arches of common carp Cyprinus carpio haematopterus (Temminck and Schlegel), causing serious pathological effects on host fish. An inadequate original description and absence of molecular data make accurate early diagnosis challenging. To augment the original description, M. shantungensis is redescribed here using morphological and molecular biological methods. Mature spores of M. shantungensis were ellipsoidal or apple shaped in frontal view and lemon shaped in lateral view, averaging 8.2  ±  0.3 μm (8.0-9.0 μm) ×  10.1 ±  0.5 μm (9.2-11.1 μm)  ×  6.9  ±  0.3 μm (6.0-7.4 μm). Some spores had three to four "V"-shaped valve edge markings on the posterior of the spore. The two equal polar capsules were oval, measuring 4.3  ±  0.3 μm (3.8-5.0 μm)  ×  3.2  ±  0.2 μm (2.8-3.5 μm), situated at the anterior extremity of the spore. Polar filaments coiled with six to seven turns. Scanning electron microscopy revealed that the surface of mature spores of M. shantungensis was generally pitted with a number of irregular ridges in shape. M. shantungensis is also characterized on the molecular level using the sequence of the small subunit ribosomal RNA gene. A BLAST search revealed that this sequence did not match any available sequences in GenBank.

  1. Genetic polymorphisms of CASR and cancer risk: evidence from meta-analysis and HuGE review

    Directory of Open Access Journals (Sweden)

    Jeong S

    2016-02-01

    Full Text Available Sohyun Jeong, Jae Hyun Kim, Myeong Gyu Kim, Nayoung Han, In-Wha Kim, Therasa Kim, Jung Mi Oh College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, South Korea Background: CASR gene appears to be involved in cancer biology and physiology. However, a number of studies investigating CASR polymorphisms and cancer risks have presented inconclusive results. Thus, a systematic review and a meta-analysis of the effect of CASR polymorphisms on several cancer risks were performed to suggest a statistical evidence for the association of CASR polymorphisms with cancer risks.Methods: MEDLINE, EMBASE, Web of Science, Scopus, and the HuGE databases were searched. Nineteen articles of case–control and cohort studies were included for the final analysis.Results: The colorectal cancer risk was reduced in proximal (odds ratio [OR] =0.679, P=0.001 and distal (OR =0.753, P=0.026 colon sites with GG genotype of CASR rs1042636 and increased in distal colon site (OR =1.418, P=0.039 with GG genotype of rs1801726 by additive genetic model. The rs17251221 demonstrated noticeable associations that carrying a homozygote variant increases breast and prostate cancer risk considerably.Conclusion: The significant association of CASR polymorphisms with several cancer risks was observed in this review. In particular, the act of CASR polymorphisms as a tumor suppressor or an oncogene differs by cancer site and can be the research target for tumorigenesis. Keywords: rs1042636, rs1801725, rs1801726, systematic review, colorectal cancer

  2. The Utility of Human Plasma-Derived Butyrylcholinesterase (huBuChE) as a Therapeutic Measure in the Absence of Pre-Treatment or Conventional Post-Poisoning Therapies Against Nerve Agent

    Science.gov (United States)

    2011-10-01

    Human butyrylcholinesterase (huBuChE) has investigational new drug (IND) status in the U.S. as a pretreatment against organophosphate poisoning in humans...huBuChE) as a therapeutic measure in the absence of pre-treatment or conventional post- poisoning therapies against nerve agent. PRINCIPAL...absence of pre- treatment or conventional post- poisoning therapies against nerve agent. 5a. CONTRACT NUMBER W81WXH-10- -0044 5b. GRANT NUMBER 5c

  3. A.~()HU

    African Journals Online (AJOL)

    officer training college to improve the professional ... exercises'. ... in 1924 of the first Labour Government, headed by ... infantry tactics and stressed riding skills. At heart, ... sergeant and cadet Prince Henry, third son of .... 'Vorld, are led by men trained at Sandhurst (for stu- .... lurst does not need a 'system' to maintain its high.

  4. 论史铁生的爱情婚姻观%On Shi Tiesheng’s Theory of Love and Marriage

    Institute of Scientific and Technical Information of China (English)

    戚国华

    2014-01-01

    爱情与婚姻在史铁生“心魂”的执着追问下焕发出奇异的神彩,他认为“残疾与爱情是上帝为人性写下的最本质的两条密码”,爱情既是人的孤独体现,也是对人类残疾拯救的途径。他常引用圣经原文,以之为比照、映衬、要求,试图印证爱情与婚姻在上帝的原初设计中是统一的,男女只有在心魂合一中方能体味到爱的真谛,以此纠正人的恶与疏失,暗示出理想的爱情婚姻应有神圣根基,引发人们对爱情与婚姻本原的沉思与反省。%Love and marriage are the topics glittering in Shi Tiesheng’s persistent“soul-searching”articles. He believed that disability and love are the two essential ciphers that God has written for humanity. Love can not only reflect human loneliness, but also serve as means to rescue human disability. Often he used biblical references to compare, set off and require, trying to confirm that love and marriage were unified in God's original design and that only with the unification of heart and soul can men and women experience true love. With this theory he meant to redress human ill behavior, suggest the sacred foundation that the ideal marriage should have, trigger the introspection on the nature of secular love and marriage.

  5. Thermal annealing and SHI irradiation induced modifications in sandwiched structured Carbon-gold-Carbon (a-C/Au/a-C) nanocomposite thin film

    Science.gov (United States)

    Singh, S. K.; Singhal, R.

    2017-09-01

    In the present work, we study the annealing and swift heavy ion (SHI) beam induced modifications in the optical and structural properties of sandwiched structured Carbon-gold-Carbon (a-C/Au/a-C) nanocomposite (NCs) thin films. The NCs thin films were synthesized by electron-beam evaporation technique at room temperature with ∼30 nm thickness for both carbon layer and ∼6 nm for gold layer. Gold-carbon NCs thin films were annealed in the presence of argon at a temperature of 500 °C, 600 °C and 750 °C. The NCs thin films were also irradiated with 90 MeV Ni ions beam with different ion fluences in the range from 3 × 1012, 6 × 1012 and 1 × 1013 ions/cm2. Surface plasmon resonance (SPR) of Au nanoparticles are not observed in the pristine film but, after annealing at temperature of 600 °C and 750 °C, it was clearly seen at ∼534 nm as confirmed by UV-visible absorption spectroscopy. 90 MeV Ni irradiated thin film at the fluence of 1 × 1013 ions/cm2 also show strong absorption band at ∼534 nm. The growth and size of Au nanoparticle for pristine and 90 MeV Ni ion irradiated thin film with fluence of 1 × 1013 ions/cm2, were estimated by Transmission electron microscopy (TEM) images with the bi-model distribution. The size of the gold nanoparticle (NPs) was found to be ∼4.5 nm for the pristine film and ∼5.4 nm for the irradiated film at a fluence of 1 × 1013 ions/cm2. The thickness and metal atomic fraction in carbon matrix were estimated by Rutherford backscattering spectroscopy (RBS). The effect of annealing as well as heavy ion irradiation on D and G band of carbon matrix were studied by Raman spectroscopy.

  6. Comment on Incomplete Characters in Novels by Shi Jie%评石杰小说中的残缺人物形象

    Institute of Scientific and Technical Information of China (English)

    杨晓梅

    2012-01-01

    石杰小说中具有大量的残缺人物形象。苦难、神秘、和谐是他们的共同特征。他们承受着命运的捉弄、他人的歧视以及道德与本能的冲突所引发的痛苦,令读者产生同情和怜悯。他们的相貌习性、感知能力和对世界的认识又呈现出神秘性,给作品增添了艺术魅力。安详的生活情态、对生死的顺应和与自然之间的融洽更为小说增添了和谐美,强化了作品的认识和审美功能。所有这些,都来源于作者对底层人的关怀之心及其所受到的传统思维方式的影响。%There are a large number of incomplete characters in Shi Jie's novels.Suffering,mysterious and harmony is their common features.They suffered from twist of fate,discrimination as well as the pain caused by the instinct and moral conflicts,which made the reader's sympathy and compassion.Their appearance and habits,perception and understanding of the world take on mysterious,which added artistic charm to the works.Serene living modality,the adaptability of life and death and harmonious between natures also added harmonious beauty to the novels and strengthened the awareness and aesthetic features of the works.All these are derived from the author's concern for the people at the bottom of the society and the influence of the traditional modes of thought.

  7. On the Generation of SHI Zhe -cun' s Historical Fictions Narrative Pattern%论施蛰存历史小说叙事模式的生成

    Institute of Scientific and Technical Information of China (English)

    周晓秋

    2012-01-01

    From the subject dimensions, the paper analyses the generation of Shi Zhe - cun' s historical fictions narrative pattern. According to the tendency of personality, he had the traits of loneliness and flinches, which indicated his introversion personality. So that he like focusing on the human beings who is the subject of activities. According to psychological needs, he grew up in the circumstance where his basic need of human was met, so the self- actualization need become to the centre of his con sciousness. He wanted to create a new writing - method which was different from others. According to personal experiences, he translated a series literary works, which made him to hold the psychoanalytical writing - method and put it into practice, so create a vivid modern narrative pattern of historical fictions.%基于主体维度,施蛰存历史小说叙事模式可以从以下三个方面来探究。就人格倾向来看,施蛰存具有孤独、退守等特质,在心理类型上属于内倾型人格,在叙事上倾向于叙述作为活动主体的人;就心理需要来看,施蛰存生长在基本需要相对满足的环境,自我实现在当时成为意识中心。在创作上,意图开辟一条新路径;就个人经验来看,施蛰存通过作品翻译,掌握了西方现代心理分析创作的方法,并将它运用于实践,创作出迥异于传统历史小说的叙事文本。

  8. 《伤逝》中现代性的三维%The Three-dimensions of Modernity in the Novel Shang Shi

    Institute of Scientific and Technical Information of China (English)

    金韬

    2017-01-01

    Shang Shi is probably the most unique in Lu Xun's novels. It is the only novel with the theme of love, and its style is very different from Lu Xun's other novels. It is a romantic novel, still a mirror to the Chinese society of that time. From the surface of tragedy, it emphasizes the importance of "not to say", that is, the orientation of value in modernity. In writing this novel, Lu Xun understood the modernity with complexity, including at least autonomy, self-reflexity and sociality. It is the greatest insight of this novel, and still has practical significance in our times.%在鲁迅的众多小说中,《伤逝》可能是最独特的一篇。这是鲁迅唯一一篇以爱情为主题的小说,其文风与作者的大多数文章有很大的区别。《伤逝》并不是简单的爱情小说,它更是具有极强的现实指向。在表面的爱情悲剧之下,《伤逝》更强调“未说”的重要性,也就是现代性价值的去向。鲁迅在建构这篇小说时,采用了复调的现代性理解,至少包括了个体自主性、自反性、社会性三个不同的现代性维度。这是这篇小说的伟大之处,也是至今仍具有现实意义的重要原因。

  9. Exploring Space Landscape and Environmental Behavior of Wang Shi Yuan Garden%网师园的空间景观与环境行为探究

    Institute of Scientific and Technical Information of China (English)

    陈思; 马子龙; 曹磊

    2012-01-01

    从网师园的历史沿革、造园立意、平面布局、主要景观及空间特色等方面分析了园内环境景观与古代文人雅士的居、息、游、赏,以及现代游览者领略文化、休闲游园等行为活动之间的关系。探究了“因诗成景”、“以画入园”的独特景观营造思想和布局手法及其在有限空间的无限意境中提高人们感知、体验、参与环境的行为价值。最后结合当下的时代精神,提炼了中国古典园林中的景观环境行为理念。%From Wang Shi Yuan's history, gardening conception,layout, major landscapes and space characteristics,etc. ,this artiCle aims at exploring its relationship between environmental landscape and activities of ancient scholars' residing, resting,touring, and enjoying as well as contemporary tourists' culture appreciation and recreation. In addition, the article explores the unique landscape thoughts and layout techniques of "poems ,of the scene" and "paintings of the garden", and improves the behavior value of people's perception, experience, and environmental participation in the infinite artistic landscape. Hopefully, according to the current spirit, the refining of the environmental behavior principles in the Chinese classical gardens will benefit the modern landscape design.

  10. Dispersion, speciation and adsorption treatment of heavy metals in the vicinity of the Shi-Heung Cu-Pb-Zn mine

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Ho Song; Chon, Hyo Taek [Seoul National Univ., Seoul (Korea, Republic of)

    1995-10-01

    In order to investigate the dispersion patterns and speciation of Cu, Pb, Zn and Cd in soils, stream sediments and stream waters, geochemical studies of soil, stream sediment and stream water samples collected in the vicinity of the Shi-Heung Cu-Pb-Zn mine was carried out. Cation exchange capacity measurement, size analysis, X-ray diffraction analysis and batch test were performed to select applicable soil for adsorption treatment. The average content of Cu, Pb, Zn and Cd in soils collected from tailings and ore dressing plant is 1084 ppm, 2292 ppm, 3512 ppm and, 29.2 ppm, respectively, and therefore, tailings and ore dressing plant site may be the major contamination sources in this study area. The mean content of Cu, Pb, Zn and Cd in stream sediments is extremely high up to 794 ppm, 1633 ppm, 2946 ppm and 25.2 ppm, respectively. Tailing particles and heavy metal ions are dispersed along the tributary system. Results from the sequential extraction analysis indicate; (1) most of Cu is bound to organic matters and sulphides, (2) fraction of Pb is mainly bound to Fe and Mn oxides. Most of Zn is largely bound to Fe and Mn oxides and residual fraction. Ion exchangeable fraction of Cd is relatively higher than those of Cu, Pb and Zn. Batch test on soils collected from the kaolinite and/or pyrophyllite mines and from the control areas was carried out to select an applicable soil samples for adsorption treatment. The sample, S10, collected from the control area 2 (clay content 33.2%) shows the highest K{sub d} (distribution coefficient). Organic content in soils and several clay minerals shows relatively good correlation with K{sub d}. It means that applicable soils for adsorption treatment of heavy metals show high organic and clay content. (author). 37 refs., 9 tabs., 6 figs.

  11. Cardiovascular effects of ethanol extract of Rubus chingii Hu (Rosaceae) in rats: an in vivo and in vitro approach.

    Science.gov (United States)

    Su, X H; Duan, R; Sun, Y Y; Wen, J F; Kang, D G; Lee, H S; Cho, K W; Jin, S N

    2014-06-01

    Rubus chingii Hu (Rosaceae) is an important traditional Chinese medicine that has been used to improve function of the kidney and treat excessive polyuria. However, the effects of Rubus chingii on the cardiovascular system and its pharmacological mechanisms of action have not been studied. The aim of the present study was to evaluate the cardiovascular effects of ethanol extract of Rubus chingii (ERC) in rats. The changes in systolic blood pressure and heart rate of rats and vascular tone of aortic rings in in vitro were measured using pressure transducer and force transducer, respectively, connected to a multichannel recording system. ERC decreased systolic blood pressure and heart rate in a concentration-dependent manner. ERC induced vasorelaxation in a concentration-dependent manner. The ERC-induced vasorelaxation was not observed in the absence of the endothelium. The vasorelaxant effect of ERC was significantly attenuated by inhibition of endothelial NO synthase (eNOS), soluble guanylyl cyclase (sGC), or Ca(2+) entry from extracellular sources with L-NAME, ODQ, diltiazem, or extracellular Ca(2+) depletion, respectively. Similarly, an inhibition of Akt with wortmannin attenuated the ERC-induced vasorelaxation. Modulators of the store-operated Ca(2+) entry, thapsigargin, Gd(3+), and 2-aminoethyl diphenylborinate markedly attenuated the ERC-induced vasorelaxation. Furthermore, 4-aminopyridine an inhibitor of voltage-dependent K(+) (KV) channel, significantly attenuated the ERC-induced vasorelaxation. However, tetraethylammonium and glibenclamide, had no significant effect on the ERC-induced vasorelaxation. Indomethacin, atropine, and propranolol had no effects on the ERC-induced vasorelaxation. The present study demonstrates that ERC induces vasorelaxation via endothelium-dependent two-step signaling: an activation of the Ca(2+)-eNOS-NO signaling in the endothelial cells and then subsequent stimulation of the NO-sGC-cGMP-KV channel signaling in the vascular

  12. A biblioterapia na humanização da assistência hospitalar do Hospital Universitário da Universidade Federal de Santa Catarina – HU/UFSC/Bibliotherapy for the hospital care humanization in the university hospital of Santa Catarina Federal University – HU/UFSC

    Directory of Open Access Journals (Sweden)

    Eva Seitz

    2008-01-01

    Full Text Available Este artigo tem por finalidade apresentar resultados do estudo realizado com pacientes, acompanhantes e funcionários das Unidades de Internação Cirúrgica do Hospital Universitário da Universidade Federal de Santa Catarina (UIC/HU/UFSC. O foco central foi o de verificar até que ponto a prática biblioterapêutica e as atividades de lazer contribuem no processo de Humanização da Assistência Hospitalar dos pacientes internados nas (UIC/HU/UFSC, na percepção dos pacientes, acompanhantes, funcionários da enfermagem e assistentes sociais da referida unidade de internação. Foram entrevistados 16 pacientes, 11 acompanhantes e 13 funcionários da enfermagem e assistentes sociais. As atividades de lazer analisadas neste estudo foram a prática biblioterapêutica, as atividades do grupo Reaja e a dança. Trata-se de uma pesquisa quantitativa e qualitativa. Quanto ao procedimento técnico, a presente pesquisa é classificada como estudo de caso, no qual, utilizou-se, para a coleta dos dados a entrevista estruturada e, cujos resultados demonstram que as atividades contribuem muito na Humanização da Assistência Hospitalar dos pacientes internados na UIC/HU/UFSC. This article has for purpose to present resulted of the study carried through with patients, companions and employees of the Units of Surgical Internment of the University Hospital of the Federal University of Santa Catarina (UIC/HU/UFSC. The central focus was to verify until point the practical library-therapy and the activities of leisure contribute in the process of Humanization of the Hospital Assistance of the patients interned in (UIC/HU/UFSC, in the perception of the patients, companions, employees of the nursing and social assistants of the related unit of internment. 16 patients, 11 companions and 13 social employees of the nursing and assistants had been interviewed. The analyzed activities of leisure in this study had been the practical library-therapy, the activities of

  13. Anti-Hu,Yo antibodies in patients with positive serum AQP4 antibody%水通道蛋白-4抗体阳性患者血清抗Hu、Yo抗体的检测

    Institute of Scientific and Technical Information of China (English)

    龙友明; 单福兰; 解龙昌; 陈梦宇; 郑杨波; 高聪; 杨宁

    2014-01-01

    Objective To evaluate the positive rates of serum anti‐Hu , and anti‐Yo antibodies in patients with positive serum aquaporin‐4 (AQP4) antibodies .Methods A consecutive cohort of 80 subjects were analyzed retrospectively . First , sera of patients were tested for AQP4 antibodies in an indirect immunofluorescence assay employing HEK‐293 cells transected with recombinant human AQP4 .Second ,anti‐Hu ,and anti‐Yo antibodies were tested with monkey cerebellum substrate by an indirect immunofluorescence assay .Results Eighty patients with positive AQP4 antibodies were confirmed in the present study .Only one of the examined sera was positive for anti‐Yo antibody (1.25% , 1/80 ) and none was positive for anti‐Hu antibodies .This patient suffered from optic neuritis and transverse myelitis , ranging over three vertebral segments on spinal MRI .Conclusions Anti‐Hu and anti‐Yo antibodies were rare in our patients with positive serum AQP4 antibodies .%目的:观察抗 Hu 抗体和抗 Yo 抗体在水通道蛋白‐4(AQP4)抗体阳性患者中的出现情况。方法检测收集80例 AQP4抗体阳性患者,以间接免疫荧光方法检血清抗 Hu 抗体、Yo 抗体,并回顾性分析抗 Hu 抗体、抗 Yo 抗体阳性患者的临床资料及诊断。 AQP4抗体检测:先以转染 AQP4基因的人胚肾293细胞结合血清中的 AQP4抗体,之后采用免疫荧光方法检测;抗 Hu 抗体、抗 Yo 抗体检测:先以猴小脑冰冻切片结合血清中的抗体,之后采用免疫荧光法检测。结果80例患者中,均未检测到抗 Hu 抗体,仅1例 NMO 患者血清抗 Yo 抗体阳性。结论抗 Hu 抗体和抗 Yo 抗体在无肿瘤的 AQP4抗体阳性患者中少见。

  14. Generation of a infectious cDNA clone of highly pathogenic porcine reproductive and respiratory syndrome virus HuN4%高致病性猪繁殖与呼吸综合征病毒HuN4株感染性分子克隆的建立及拯救病毒的鉴定

    Institute of Scientific and Technical Information of China (English)

    张善瑞; 周艳君; 朱建平; 童武; 姜一峰; 童光志

    2011-01-01

    In this study, a infectious cDNA clone of highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) strain HuN4 was generated by cloning of the 6 overlapped RT-PCR fragments into pBlueScript II SK(+), designated pHuN4. In addition, sp6 promoter at 5'-terminus and a Not I site in Poly(A) tail at 3'-terminus were introduced into the cDNA of PRRSV for viral RNA transcription in vitro and the pHuN4 linearization, respectively. Furthmore, the A at 14,680 nt was replaced by G (silent mutation) to generate restriction enzyme Mlu I site as molecular marker for identification of rescued virus. The pHuN4 was linearized and transcribed in vitro, the transcripts were transfected primarily into BHK cells and then Marc 145 cells, the virus was successfully rescued from Marc 145 cells identified by indirect immofluorescent assay and Mlu I digestion. The rescued virus showed similar growth curve to its parental virus HuN4 strain. All the piglets infected with the fifth passage of the rescued virus developed clinical signs at 3 day-past-infection and died within 20 days. The results showed that the rescued virus remained similar biological characterizations as its parental virus. The pHuN4 developed in the study by reverse genetic system might provide a platform for further study on pathogenesis mechanism of highly pathogenic PRRSV.%为构建高致病性猪繁殖与呼吸综合征病毒(PRRSV)感染性克隆,本实验根据PRRSV HuN4株基因组序列设计并合成PRRSV特异性引物,应用RT-PCR技术分6段扩增了PRRSV HuN4株全基因组cDNA.将扩增的各个cDNA部分重叠片段分别克隆于pBlueScript Ⅱ SK(+)载体中构建了感染性重组质粒pHuN4.并在病毒cDNA 5′末端引入sp6启动子序列便于后期的体外转录获得病毒的转录本,在3'末段Poly (A)尾引入Not Ⅰ酶切位点用于线性化pHuN4;此外,将HuN4基因组第14680位的A沉默突变为G产生一个Mlu Ⅰ酶切位点作为鉴定拯救病毒的分子标记.pHu

  15. Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by a new fungal strain Cladosporium cladosporioides Hu-01.

    Science.gov (United States)

    Chen, Shaohua; Liu, Chenglan; Peng, Chuyan; Liu, Hongmei; Hu, Meiying; Zhong, Guohua

    2012-01-01

    Intensive use of chlorpyrifos has resulted in its ubiquitous presence as a contaminant in surface streams and soils. It is thus critically essential to develop bioremediation methods to degrade and eliminate this pollutant from environments. We present here that a new fungal strain Hu-01 with high chlorpyrifos-degradation activity was isolated and identified as Cladosporium cladosporioides based on the morphology and 5.8S rDNA gene analysis. Strain Hu-01 utilized 50 mg·L(-1) of chlorpyrifos as the sole carbon of source, and tolerated high concentration of chlorpyrifos up to 500 mg·L(-1). The optimum degradation conditions were determined to be 26.8°C and pH 6.5 based on the response surface methodology (RSM). Under these conditions, strain Hu-01 completely metabolized the supplemented chlorpyrifos (50 mg·L(-1)) within 5 d. During the biodegradation process, transient accumulation of 3,5,6-trichloro-2-pyridinol (TCP) was observed. However, this intermediate product did not accumulate in the medium and disappeared quickly. No persistent accumulative metabolite was detected by gas chromatopraphy-mass spectrometry (GC-MS) analysis at the end of experiment. Furthermore, degradation kinetics of chlorpyrifos and TCP followed the first-order model. Compared to the non-inoculated controls, the half-lives (t(1/2)) of chlorpyrifos and TCP significantly reduced by 688.0 and 986.9 h with the inoculum, respectively. The isolate harbors the metabolic pathway for the complete detoxification of chlorpyrifos and its hydrolysis product TCP, thus suggesting the fungus may be a promising candidate for bioremediation of chlorpyrifos-contaminated water, soil or crop.

  16. El farmacéutico en el contexto de las enfermedades raras y los medicamentos huérfanos The pharmacist, rare diseases and orphan medicines

    Directory of Open Access Journals (Sweden)

    C. Nagore

    2008-01-01

    Full Text Available El tratamiento de las enfermedades raras muchas veces resulta complicado debido a las dificultades que plantean la investigación y desarrollo de medicamentos huérfanos por parte de la industria farmacéutica. Una de las mayores preocupaciones de este grupo de pacientes tan escaso y diverso al mismo tiempo, es el acceso a los fármacos disponibles. La Agencia Europea del Medicamento establece la denominación de medicamentos huérfanos y los incentivos y bases necesarias para su comercialización. La mayoría de los medicamentos huérfanos por sus especiales características, exigen una adecuada vigilancia y control. Los servicios de farmacia hospitalarios están activamente implicados en su gestión y dispensación, así como en un correcto seguimiento farmacoterapéutico de los pacientes.Treatment of rare diseases is often complicated due to the difficulties posed by the research and development of medicines by the pharmacological industry. One of the greatest concerns of this group of patients, which is both scarce and diverse, is access to available medicines. The European Medicine Agency establishes the term orphan medicines and the incentives and bases for their commercialisation. Due to their special characteristics, the majority of orphan medicines require suitable vigilance and control. The hospital pharmacy services are actively involved in their management and supply, as well as in a correct pharmacotherapeutic monitoring of the patients.

  17. Exploiting a novel miR-519c-HuR-ABCG2 regulatory pathway to overcome chemoresistance in colorectal cancer.

    Science.gov (United States)

    To, Kenneth K W; Leung, W W; Ng, Simon S M

    2015-11-01

    Colorectal cancer (CRC) is a major cause of mortality and morbidity worldwide. While surgery remains the mainstay of treatment for early stage CRC, adjuvant chemotherapy is usually given to reduce the risk of recurrence after colectomy. Overexpression of a multidrug resistance (MDR) transporter ABCG2 in vitro has been shown to cause resistance to 5-fluorouracil (5-FU) and irinotecan, components of the most commonly adopted regimens for treating CRC. Both anticancer drugs are known ABCG2 substrates. An effective way to predict drug response may provide guidance for better cancer treatment. We investigated the effect of ABCG2 dysregulation on cancer cell sensitivity to chemotherapy using pairs of snap-frozen paraffin-embedded archival blocks of human colorectal cancer tissues and their matched non-cancerous colon tissues from CRC patients. In CRC patients responding to chemotherapy, the tumors were found to have remarkable lower ABCG2 expression than the adjacent normal colon tissues. On the contrary, the tumors from patients not responding to 5-FU-based chemotherapy have higher ABCG2 level than the adjacent normal tissues. The high ABCG2 expression in the tumor is associated with the concomitant overexpression of the mRNA binding protein HuR but a low expression of miR-519c because miR-519c is known to target both ABCG2 and HuR. Further investigation in CRC cell lines revealed that the ABCG2 overexpression was caused by an interplay between miR-519c, HuR and the length of the 3' untranslated region (UTR) of ABCG2. These parameters may be further developed as useful biomarkers to predict patient response to adjuvant chemotherapy. Besides being predictive biomarkers, the microRNAs and mRNA binding protein identified may also be potential drug targets for modulating ABCG2 to combat resistance in CRC chemotherapy.

  18. ARG098, a novel anti-human Fas antibody, suppresses synovial hyperplasia and prevents cartilage destruction in a severe combined immunodeficient-HuRAg mouse model

    Directory of Open Access Journals (Sweden)

    Matsubara Tsukasa

    2010-09-01

    Full Text Available Abstract Background The anti-human Fas/APO-1/CD95 (Fas mouse/human chimeric monoclonal IgM antibody ARG098 (ARG098 targets the human Fas molecule. The cytotoxic effects of ARG098 on cells isolated from RA patients, on normal cells in vitro, and on RA synovial tissue and cartilage in vivo using implanted rheumatoid tissues in an SCID mouse model (SCID-HuRAg were investigated to examine the potential of ARG098 as a therapy for RA. Methods ARG098 binding to each cell was analyzed by cytometry. The effects of ARG098 on several cells were assessed by a cell viability assay in vitro. Effects on the RA synovium, lymphocytes, and cartilage were assessed in vivo using the SCID-HuRAg mouse model. Results ARG098 bound to cell surface Fas molecules, and induced apoptosis in Fas-expressing RA synoviocytes and infiltrating lymphocytes in the RA synovium in a dose-dependent manner. However, ARG098 did not affect the cell viability of peripheral blood mononuclear cells of RA patients or normal chondrocytes. ARG098 also induced apoptosis in RA synoviocytes and infiltrating lymphocytes in the RA synovium in vivo. The destruction of cartilage due to synovial invasion was inhibited by ARG098 injection in the modified SCID-HuRAg mouse model. Conclusions ARG098 treatment suppressed RA synovial hyperplasia through the induction of apoptosis and prevented cartilage destruction in vivo. These results suggest that ARG098 might become a new therapy for RA.

  19. Neuroblastoma amplified sequence gene is associated with a novel short stature syndrome characterised by optic nerve atrophy and Pelger-Huët anomaly.

    Science.gov (United States)

    Maksimova, Nadezda; Hara, Kenju; Nikolaeva, Irina; Chun-Feng, Tan; Usui, Tomoaki; Takagi, Mineo; Nishihira, Yasushi; Miyashita, Akinori; Fujiwara, Hiroshi; Oyama, Tokuhide; Nogovicina, Anna; Sukhomyasova, Aitalina; Potapova, Svetlana; Kuwano, Ryozo; Takahashi, Hitoshi; Nishizawa, Masatoyo; Onodera, Osamu

    2010-08-01

    Hereditary short stature syndromes are clinically and genetically heterogeneous disorders and the cause have not been fully identified. Yakuts are a population isolated in Asia; they live in the far east of the Russian Federation and have a high prevalence of hereditary short stature syndrome including 3-M syndrome. A novel short stature syndrome in Yakuts is reported here, which is characterised by autosomal recessive inheritance, severe postnatal growth retardation, facial dysmorphism with senile face, small hands and feet, normal intelligence, Pelger-Huët anomaly of leucocytes, and optic atrophy with loss of visual acuity and colour vision. This new syndrome is designated as short stature with optic atrophy and Pelger-Huët anomaly (SOPH) syndrome. To identify a causative gene for SOPH syndrome. Genomewide homozygosity mapping was conducted in 33 patients in 30 families. The disease locus was mapped to the 1.1 Mb region on chromosome 2p24.3, including the neuroblastoma amplified sequence (NBAS) gene. Subsequently, 33 of 34 patients were identified with SOPH syndrome and had a 5741G/A nucleotide substitution (resulting in the amino acid substitution R1914H) in the NBAS gene in the homozygous state. None of the 203 normal Yakuts individuals had this substitution in the homozygous state. Immunohistochemical analysis revealed that the NBAS protein is well expressed in retinal ganglion cells, epidermal skin cells, and leucocyte cytoplasm in controls as well as a patient with SOPH syndrome. These findings suggest that function of NBAS may associate with the pathogenesis of short stature syndrome as well as optic atrophy and Pelger-Huët anomaly.

  20. Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by a new fungal strain Cladosporium cladosporioides Hu-01.

    Directory of Open Access Journals (Sweden)

    Shaohua Chen

    Full Text Available Intensive use of chlorpyrifos has resulted in its ubiquitous presence as a contaminant in surface streams and soils. It is thus critically essential to develop bioremediation methods to degrade and eliminate this pollutant from environments. We present here that a new fungal strain Hu-01 with high chlorpyrifos-degradation activity was isolated and identified as Cladosporium cladosporioides based on the morphology and 5.8S rDNA gene analysis. Strain Hu-01 utilized 50 mg·L(-1 of chlorpyrifos as the sole carbon of source, and tolerated high concentration of chlorpyrifos up to 500 mg·L(-1. The optimum degradation conditions were determined to be 26.8°C and pH 6.5 based on the response surface methodology (RSM. Under these conditions, strain Hu-01 completely metabolized the supplemented chlorpyrifos (50 mg·L(-1 within 5 d. During the biodegradation process, transient accumulation of 3,5,6-trichloro-2-pyridinol (TCP was observed. However, this intermediate product did not accumulate in the medium and disappeared quickly. No persistent accumulative metabolite was detected by gas chromatopraphy-mass spectrometry (GC-MS analysis at the end of experiment. Furthermore, degradation kinetics of chlorpyrifos and TCP followed the first-order model. Compared to the non-inoculated controls, the half-lives (t(1/2 of chlorpyrifos and TCP significantly reduced by 688.0 and 986.9 h with the inoculum, respectively. The isolate harbors the metabolic pathway for the complete detoxification of chlorpyrifos and its hydrolysis product TCP, thus suggesting the fungus may be a promising candidate for bioremediation of chlorpyrifos-contaminated water, soil or crop.

  1. A novel cantharidin analog N-Benzylcantharidinamide reduces the expression of MMP-9 and invasive potentials of Hep3B via inhibiting cytosolic translocation of HuR

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ji-Yeon; Chung, Tae-Wook; Choi, Hee-Jung [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Lee, Chang Hyun [Department of Anatomy, College of Korean Medicine, Woosuk University, Wanju-gun, Jeonbuk (Korea, Republic of); Eun, Jae Soon; Han, Young Taek [College of Pharmacy, Woosuk University, Wanju-gun, Jeonbuk (Korea, Republic of); Choi, Jun-Yong [Department of Internal Medicine, Korean Medicine Hospital, School of Korean Medicine, Pusan National University, Yangsan 626-870 (Korea, Republic of); Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Kim, So-Yeon; Han, Chang-Woo [Department of Internal Medicine, Korean Medicine Hospital, School of Korean Medicine, Pusan National University, Yangsan 626-870 (Korea, Republic of); Jeong, Han-Sol, E-mail: jhsol33@pusan.ac.kr [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Ha, Ki-Tae, E-mail: hagis@pusan.ac.kr [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of)

    2014-05-02

    Highlights: • We examined the inhibition of N-Benzylcantharidinamide on MMP-9-mediated invasion. • Unlike cantharidin, N-Benzylcantharidinamide has very low toxicity on Hep3B cells. • The reduced MMP-9 expression was due to HuR-mediated decrease of mRNA stability. • We suggest N-Benzylcantharidinamide as a novel inhibitor of MMP-9-related invasion. - Abstract: Invasion and metastasis are major causes of malignant tumor-associated mortality. The present study aimed to investigate the molecular events underlying inhibitory effect of N-Benzylcantharidinamide, a novel synthetic analog of cantharidin, on matrix metalloproteinase-9 (MMP-9)-mediated invasion in highly metastatic hepatocellular carcinoma Hep3B cells. In this investigation, among six analogs of cantharidin, only N-Benzylcantharidinamide has the inhibitory action on MMP-9 expression at non-toxic dose. The MMP-9 expression and invasion of Hep3B cells were significantly suppressed by treatment of N-Benzylcantharidinamide in a dose-dependent manner. On the other hand, the transcriptional activity of MMP-9 promoter and nuclear levels of NF-κB and AP-1 as the main transcriptional factors inducing MMP-9 expression were not affected by it although the level of MMP-9 mRNA was reduced by treatment of N-Benzylcantharidinamide. Interestingly, the stability of MMP-9 mRNA was significantly reduced by N-Benzylcantharidinamide-treatment. In addition, the cytosolic translocation of human antigen R (HuR), which results in the increase of MMP-9 mRNA stability through interaction of HuR with 3′-untranslated region of MMP-9 mRNA, was suppressed by treatment of N-Benzylcantharidinamide, in a dose-dependent manner. Taken together, it was demonstrated, for the first time, that N-Benzylcantharidinamide suppresses MMP-9 expression by reducing HuR-mediated MMP-9 mRNA stability for the inhibition of invasive potential in highly metastatic Hep3B cells.

  2. Reflexiones bioéticas en torno a las enfermedades raras y huérfanas con impacto en el sistema bucodentario

    Directory of Open Access Journals (Sweden)

    Gilberto Cely Galindo

    2011-01-01

    Full Text Available Este artículo está dirigido a odontopediatras que forman parte de grupos interdisciplinarios de investigación y terapia clínica, a los pacientes y sus familias con problemas genéticos de las llamadas "enfermedades raras y huérfanas". Estas enfermedades sistémicas tienen repercusiones severas bucodentales. El énfasis lo ponemos en las reflexiones bioéticas que orientan la acción investigativa y terapéutica.

  3. Nuevas citas y huéspedes de Phenacoccus para la Argentina (Hemiptera: Pseudococcidae New records and host plants of Phenacoccus from Argentina (Hemiptera: Pseudococcidae

    Directory of Open Access Journals (Sweden)

    María C. Granara de Willink

    2003-12-01

    Full Text Available Se cita por primera vez para la Argentina a: Phenacoccus madeirensis Green, Phenacoccus manihoti Matile-Ferrero y Phenacoccus solenopsis Tinsley y se dan a conocer nuevos huéspedes de Phenacoccus crassus Granara de Willink, Phenacoccus parvus Morrison y Phenacoccus similis Granara de Willink.Phenacoccus madeirensis Green, Phenacoccus manihoti Matile-Ferrero and Phenacoccus solenopsis Tinsley, first records from Argentina and new hosts plants of Phenacoccus crassus Granara de Willink, Phenacoccus parvus Morrison y Phenacoccus similis Granara de Willink.

  4. Efecto de una intervención educativa sobre los conocimientos de teniasis/cisticercosis en una comunidad rural de Huánuco, Perú 2005

    OpenAIRE

    Elsa Palacios-Flores; Eler Borneo-Cantalicio

    2008-01-01

    Objetivo. Evaluar el efecto de una intervención educativa sobre los conocimientos de teniasis/cisticercosis en los pobladores de la localidad de Queropalca, Huánuco. Materiales y métodos. Se realizó un ensayo comunitario, en el cual se aplicó una estrategia educativo comunicacional sobre los factores de riesgo de teniasis/cisticercosis, consistente en metodologías participativas de visitas casa en casa y campañas de promoción en toda la jurisdicción durante seis meses (junio a diciembre del 2...

  5. Un mecenas de la educación: Francisco de Solís, fundador del Colegio Menor de Huérfanos

    Directory of Open Access Journals (Sweden)

    Miguel Á. MARTÍN SÁNCHEZ

    2009-11-01

    Full Text Available RESUMEN: Durante el siglo XVI se fundaron en la Universidad de Salamanca multitud de colegios universitarios. Uno de esos colegios, fue el menor de la Concepción de Huérfanos, primer colegio universitario que se funda para amparar niños pobres y huérfanos. El fundador de este colegio fue Francisco de Solís Quiñones y Montenegro, obispo de Bagnorea, secretario y médico particular de Paulo III, entre otras cosas. Este mecenas de la educación, decidió invertir su fortuna en la construcción y dotación de un colegio donde niños pobres y huérfanos, preferiblemente de la ciudad de Salamanca, pudiesen estudiar en la Universidad hasta graduarse en la ciencia que eligiesen. La figura de tan ilustre salmantino ha pasado prácticamente desapercibida, a pesar de su relevancia. Con este trabajo se pretende dar a conocer una de las personalidades más relevantes del panorama renacentista salmantino, por la importancia y trascendencia social y educativa que tuvo su obra.ABSTRACT: During the 16* century, a lot of colleges were founded at the University of Salamanca. One of those colleges, was the Concepción de Huérfanos Minor College, the first college that is founded for protecting poor children and orphans. This college founder was Francisco de Solís Quiñones y Montenegro, bishop of Bagnorea, secretary and personal physician of Paulo III, among other things. This patron of education, decided to spend his fortune on building and endowment of a school where poor children and orphans, in the town of Salamanca preferably, were able to go to university until they graduated in the science they chose. This Salamancan s outstanding figure has gone unnoticed in spite of his importance. In this work, it's tried to introduce one of the most important personalities in Salamancan Renaissance panorama, because of the importance and social and educative implications his work had.

  6. TLC Identification of Pomegranate Seed and Terminalia Chebula, HPLC Determination of Cinnamal and Piperine in Tibetan Medicine Shi-Wei Xiao-Shi-San%藏药十味消食散中石榴子、诃子的TLC鉴别及桂皮醛、胡椒碱的HPLC测定*

    Institute of Scientific and Technical Information of China (English)

    陈鹏; 刘媛慧; 骆桂法; 张幸福

    2014-01-01

    This study was aimed to establish a method for quality control of Tibetan medicine Shi-Wei Xiao-Shi-San (SWXSS). Thin layer chromatography (TLC) was established for the qualitative determination. Cinnamal and piperine were determined by HPLC. The separation was performed on Phenomenex C18 column (4.6 mm × 250 mm, 5μm). The results showed that TLC can be used in the identification of pomegranate seed and Terminalia chebula. The linear ranges of cinnamal and piperine were within the range of 0.06-0.37μg and 0.05-0.33μg, respectively. And the standard line was Y=9.273 2×106X-2.348 2×105, r=0.999 8;Y=7.315×103X-3.857, r=1.000 0. It was concluded that the identification method was specific, accurate and practical, which can be applied in the quality control of SWXSS.%目的:建立藏药十味消食散的质量控制方法。方法:采用薄层色谱法进行定性鉴别,采用HPLC方法测定桂皮醛和胡椒碱的含量,色谱柱为Phenomenex C18柱(250 mm×4.6 mm,5μm)。结果:TLC法可鉴别石榴子、诃子的特征斑点;桂皮醛、胡椒碱的进样量分别在0.06-0.37μg、0.05-0.33μg范围内呈良好的线性关系,回归方程分别为Y=9.2732×106X-2.3482×105,r=0.9998;Y=7.315×103X-3.857,r=1.0000。结论:鉴别方法专属性强,定量方法准确,可操作性强,可对十味消食散的质量控制提供参考。

  7. Liver iron is a major regulator of hepcidin gene expression via BMP/SMAD pathway in a rat model of chronic renal failure under treatment with high rHuEPO doses.

    Science.gov (United States)

    Ribeiro, Sandra; Garrido, Patrícia; Fernandes, João; Rocha-Pereira, Petronila; Costa, Elísio; Belo, Luís; Reis, Flávio; Santos-Silva, Alice

    2016-05-01

    Hepcidin is the major central regulator of iron metabolism, controlling iron absorption and mobilization. Considering its interaction with several factors that are altered in chronic kidney disease (CKD), particularly in hyporesponsive CKD patients under therapy with high recombinant human erythropoietin (rHuEPO) doses, it was aimed to study the impact of increasing rHuEPO doses on the regulation of iron-hepcidin metabolism. The blood, cellular, and tissue studies, using the remnant kidney rat model of CKD induced by 5/6 nephrectomy, under rHuEPO (100, 200, 400, and 600 IU/Kg body weight [BW]/week) treatment during 3 weeks were performed. It was found that the rHuEPO stimulus triggered a first wave to achieve correction of anemia, by inhibiting hepcidin synthesis, favoring erythropoiesis and iron absorption; this continuous stimulus enhanced iron absorption leading to iron overload, as showed by the hepatic iron deposits found in rats treated with higher rHuEPO dose that seems to trigger the upregulation of hepcidin synthesis through the activation of the BMP6/SMAD pathway. The data suggested that liver iron overload was an important stimuli for hepcidin synthesis, stronger than the inhibitory effect of high rHuEPO doses; moreover, the findings raised the hypothesis that when high inflammation (triggering hepcidin expression) was associated with increased iron stores in hemodialysis patients, hepcidin expression was also upregulated via BMP6, enhancing hepcidin synthesis, leading, therefore, to worsening of anemia and, eventually, to a hyporesponse/resistance to rHuEPO therapy. © 2016 BioFactors, 42(3):296-306, 2016.

  8. On ZHANG Da-qian and His Fame as“an Expert in Studies of SHI Tao”%论张大千与“石涛专家”之名

    Institute of Scientific and Technical Information of China (English)

    陈丹

    2016-01-01

    In his early years,ZHANG Da-qian was famous for imitation of SHI Tao’s works.And his imitated paintings of SHI Tao’s works were quite difficult to distinguish them from the original ones.In this sense,he was famed “an expert in studies of SHI Tao.However,he suffered a lot of controversy because of such experience.Nevertheless,the patterning and modeling after the ancient style helped him sort out the Chinese history of art,and led to his great progress in painting tech-niques.And he finally was able to integrate the ancient theories or techniques into his paintings and then make some changes. This event fully shows the complex relationship between the traditional and modern Chinese paintings in the 20 th century.%张大千早年在上海以仿石涛出名,所仿石涛画作让人真假难辨,所以被誉为“石涛专家”。一方面,摹古、仿古的经历让他饱受争议;然而另一方面,摹古、仿古又让他在技法上梳理了中国绘画史,使他的绘画技艺突飞猛进,最终能够熔古而变今。这一事件充分地表现了二十世纪中国画中传统与现代之间复杂的关系。

  9. 从“主题表演”的出现谈北狮运动创新发展方向%Talk about BeiShi in Innovation and Development Direction from the Appearance of "Theme performance"

    Institute of Scientific and Technical Information of China (English)

    杜君鹏

    2011-01-01

    随着现代北狮运动的发展,“主题表演”这一新的形式相继出现在北狮自选套路比赛的赛场。为了更加准确地分析这一创新形式为现代北狮运动发展带来的变化、客观把握其发展动向,文章收集、观看和分析了近年来的相关视频及文献资料,并结合本领域内部分专家及一线教练员的观点,分别从技术动作、音乐伴奏、场地器材的设置等方面分析了现代北狮运动的创新发展方向,旨在为其今后的创新活动提供一定的理论支持。%With the development of BeiShi, "theme performance" has arisen in the optional routines game playing. In order to analyze the change which the innovation form makes for the development of BeiShi and to grasp their development trend, the writer has done some collection, viewed and analyzed related video and literature material in recent years, and combined with the view of a part of specialists and a line of the coaches. Finally, the writer analyzed in terms of the BeiShi innovative development direction separately from the technical movements, music accompaniment, location equipment settings, aiming at providing some certain theoretical support for its future innovation activities.

  10. Discuss on Zhong Rongs Theory and Integrity of Poetry:Focused on The Preface of Shi Pin%论钟嵘的诗学观--以《诗品序》为中心

    Institute of Scientific and Technical Information of China (English)

    刘芳

    2015-01-01

    钟嵘诗歌理论在中国古代诗论的发展史上占有非常重要的地位。《诗品序》是钟嵘《诗品》中的一篇重要诗论。以《诗品序》为中心剖析钟嵘的诗学观,即:追求自然美的“自然英旨说”;情与物之“滋味说”;定品第显优劣及诗者发泄哀怨凄苍的“风力说”。%Zhong Rongs poetry theory has an important position in the development history of Chinese ancient poetry theory.The Preface of Shi Pin is an important poetics in Zhong Rongs Shi Pin .This paper starts from Zhong Rong himself,seeking the personality of his main crea-tion figures,and focusing on The Preface of Shi Pin to analyze the Zhong Rongs poetics,that is the following three major characteristics:the first is from the perspective of nature,to pur-sue true feelings expression on natural beauty;the second is from the perspective of emotion, the perceiving emotion and substances;the third is from the perspective of poems quality,to mark the rank based on the quality of poems,and to give vent to the plaintive expression for grief,resentment.

  11. Investigation on version and author of Zeng bu shi wu ben cao bei kao (Augmented Dietary Materia Medica for Investigation)%《增补食物本草备考》版本及作者考

    Institute of Scientific and Technical Information of China (English)

    郑洪; 廖春红

    2012-01-01

    Zeng bu shi wu ben cao bei kao,a masterpiece of Guangdong dietary materia medica works,has a variety of different versions.Based on our investigation on the existing versions,it is claimed that the statement in Zhong guo zhong yi gu ji zong mu (General Catalogues of Chinese Ancient Books),showing the first version of this book to be the version at the “renzi year of Emperor Yongzheng”,is wrong.We infer that the author of Zeng bu shi wu ben cao bei kao,HE Kejian and that of Sheng cao yao xing bei yao,HE Jian,is the same person.However,the former book is mainly a book on HE Ke-jian' s experiences sorted by HE Sheng-xuan on the basis of Shi wu ben cao hui zuan,and probably it maybe initially carved in 1738.%《增补食物本草备考》为广东食物本草类著作的代表作,现有多种版本存世,经考察,《中国中医古籍总目》著录该书最早版本为“雍正壬子”之说有误.此书署名作者何克谏与《生草药性备要》署名作者何谏为同一人.此书系何省轩整理何克谏经验,以《食物本草会纂》为蓝本辑编而成,可能初刻于1738年.

  12. Prevalence of swallowing and speech problems in daily life after chemoradiation for head and neck cancer based on cut-off scores of the patient-reported outcome measures SWAL-QOL and SHI.

    Science.gov (United States)

    Rinkel, Rico N; Verdonck-de Leeuw, Irma M; Doornaert, Patricia; Buter, Jan; de Bree, Remco; Langendijk, Johannes A; Aaronson, Neil K; Leemans, C René

    2016-07-01

    The objective of this study is to assess swallowing and speech outcome after chemoradiation therapy for head and neck cancer, based on the patient-reported outcome measures Swallowing Quality of Life Questionnaire (SWAL-QOL) and Speech Handicap Index (SHI), both provided with cut-off scores. This is a cross-sectional study. Department of Otolaryngology/Head and Neck Surgery of a University Medical Center. Sixty patients, 6 months to 5 years after chemoradiation for head and neck squamous cell carcinoma. Swallowing Quality of Life Questionnaire (SWAL-QOL) and SHI, both validated in Dutch and provided with cut-off scores. Associations were tested between the outcome measures and independent variables (age, gender, tumor stage and site, and radiotherapy technique, time since treatment, comorbidity and food intake). Fifty-two patients returned the SWAL-QOL and 47 the SHI (response rate 87 and 78 %, respectively). Swallowing and speech problems were present in 79 and 55 %, respectively. Normal food intake was noticed in 45, 35 % had a soft diet and 20 % tube feeding. Patients with soft diet and tube feeding reported more swallowing problems compared to patients with normal oral intake. Tumor subsite was significantly associated with swallowing outcome (less problems in larynx/hypopharynx compared to oral/oropharynx). Radiation technique was significantly associated with psychosocial speech problems (less problems in patients treated with IMRT). Swallowing and (to a lesser extent) speech problems in daily life are frequently present after chemoradiation therapy for head and neck cancer. Future prospective studies will give more insight into the course of speech and swallowing problems after chemoradiation and into efficacy of new radiation techniques and swallowing and speech rehabilitation programs.

  13. Study on Shi Bing: This Chapter wasn't Plagiarized from Fu-niao Fu%《鹖冠子·世兵》篇非抄袭贾谊《服鸟鸟赋》辨

    Institute of Scientific and Technical Information of China (English)

    杨兆贵

    2009-01-01

    Liu Zongyuan considered that the Shi Bing Chapter of Ho-Kuan-Tzu was plagiarized from Jia Yi's Fu-niao Fu because there were some same sentences between these two essays. However, this article would reveal that these two essays have used the same quotations from some classics in pre-Qin and Han dynasty, regarding three aspects: writing skills, literature style, authors' and works' thoughts and their historical settings. Therefore, the conclusion should be that they have the same theoretical resources, and were created in a close period. Moreover, Jia Yi wrote the Fu-niao Fu after reading the Shi Bing Chapter. The Shi Bing chapter was probably finished during early Han dynasty according to the study of Cao Mo's tale.%篇有些句子和贾谊相同,柳宗元就认为是抄袭,并而断定是伪书.本文从文体研究、编纂者的思想背景、撰写手法三方面来论证篇与都引用先秦秦汉的典籍,不能证明篇抄袭.相反,两者所反映的思想却有共同的时代背景,且思想重点不同.另外,篇前部分记载曹沫一事,与后部分内容无涉.本文考证曹沫一事,推论本篇写成于战国末期与汉初之间.

  14. On the width and slope at sidewalk. At Fukuoka-shi on 1998; Hodo no baria {center_dot} furi kiso chosa. Heisei 10 nen {center_dot} Fukuokashi

    Energy Technology Data Exchange (ETDEWEB)

    Iida, Toshihiko; Kojina, Kyoji

    1999-09-01

    We sta died on the width of sidewalk and the step setting slope between road and sidewalk at Fukuoka-shi on 1998 during the summer. We surveyed about 100 spots in each seven administral districts and finally collected data of 662 spots at total Fukuoka-shi. Data were devised into three items, the 1st was the width (L) of a sidewalk, the 2nd was the step (H) between road and sidewalk, and the 3rd was the length (l) of the slope between road and sidewalk. The inclination (S) is indicated for the example at a eight degree slope, slope of 1 in 12 or eight percentage. This is said that the upper limit of the inclination for the manual wheelchair is slope of 1 in 12 or eight percentage. And so the minimum of width is 1000 mm of a sidewalk. These items are definited in the standard of the law now. First we arranged these data in 13 types which were divided on acount of road and widewalk around the sites. These types were called A, B, C and so on M. The 1st much type is H and 221 spots, type H formes when sidewalk set one course at crossing site and also crosswalk only one. The 2nd much type is A and 164 spots, type A formes when sidewalk set two directions at crossing site and also cross-walk two. The 3rd much type is F and 79 spots, type F formes when crosswalk joins at site by T junction and of course sidewalk lines the site. Next we analyzed these data according the width (L) of sidewalk Case I; L {>=} 4,000 mm Case II; L < 4,000 mm Case III; slope with walking direction Then we excepted 22 samples; M type, consideration of kerbstones which were barrier-free for wheelchair, consequently data were 640 spots. As results of this study are as follows: the average ratio of sidewalks spots for barrier-free was about 50 percent at Fukuoka-shi. The same value was 45 percent on Case I, was 12 percent on Case II and was 75 percent on Case III. The Criteria of Barrier-Free Sidewalk for wheelchair in Fukuoka-shi were legislated on 1995 which set the Minimum of Width for Wheelchair

  15. Tafonomia del yacimiento de vertebrados pleistoceno de Huéscar-1 (Granada, españa

    Directory of Open Access Journals (Sweden)

    Alberdi, M. T.

    2003-08-01

    Full Text Available This work analyses the sedimentary and taphonomic processes that induced the selective preservation and spatial distribution of Húescar-1 bone assemblage (early Galerian. The bone fossil remains are associated with an alluvial fan system that flowed into a lacustrine area with calcareous sedimentation. The fossils show numerous scratch and abrasion marks produced during their transportation as channel lag. The remains appear in three sedimentological settings: in paleochannel filling-up sequences, in pockets built at the outlet of the channels in the lacustrine area, and scattered within calcareous silts. Nevertheless, most of them are found in the second instante. The cumulative process was attritional as pointed out by the mortality profiles of Equus altidens and could be explained by selective predation and/or seasonal bone accumulation. The notably small number of artiodactyl species, particularly in the typical foms of bush or forest habitats and the presence of amphibious forms (Hippopotamus are indicative of an open grassland habitat. The aquatic birds represent the autochthonous component of the association. For each bone recovered severa1 variables were measured, the data obtained underwent graphic and statistical treatment that provided useful information for the site taphonomic interpretation.Se estudia el conjunto de procesos sedimentarios y rasgos tafonómicos que condicionaron la preservación selectiva y disposición espacial de los restos de vertebrados que forman el yacimiento de edad Galeriense inferior de Huéscar-l. Los restos óseos están asociados a un sistema de abanicos aluviales que desembocaba en una zona lacustre carbonatada amplia. Es un conjunto transportado donde los restos muestran numerosas señales de abrasión y arrastre. Los restos óseos aparecen en tres situaciones sedimentológicas: carga de fondo en el relleno de los paleocanales, bolsadas formadas en el punto de la desembocadura y dispersos espor

  16. A frameshift mutation in the HuP2 paired domain of the probable human homolog of murine Pax-3 is responsible for Waardenburg syndrome type 1 in an Indonesian family.

    Science.gov (United States)

    Morell, R; Friedman, T B; Moeljopawiro, S; Hartono; Soewito; Asher, J H

    1992-07-01

    Waardenburg syndrome type 1 (WS1) is an autosomal dominant disorder characterized by deafness, dystopia canthorum, heterochromia iridis, white forelock, and premature greying. A similar phenotype is caused in the mouse by mutations in the Pax-3 gene. This observation, together with comparisons of conserved syntenies in the murine and human genetic maps, suggested that at least some WS1 mutations should occur in HuP2, the probable human homolog of Pax-3. Two mutations in the HuP2 sequence of individuals with WS1 have been reported recently. Both of them occur in the highly conserved paired box region of the gene, which encodes a DNA binding domain. The functional consequences of these mutations are at present speculative. We report here a 14 bp deletion in the paired domain encoded by exon 2 of HuP2 in an Indonesian family segregating for WS1. This frameshift mutation results in a premature termination codon in exon 3. The HuP2 product is a truncated protein lacking most of the paired domain and all of the predicted homeo domain. We propose that the WS1 phenotype in this family is due to loss of function of HuP2 and discuss two mechanisms for the dominant effect of this mutation.

  17. Tissue-specific promoter usage and diverse splicing variants of found in neurons, an ancestral Hu/ELAV-like RNA-binding protein gene of insects, in the direct-developing insect Gryllus bimaculatus.

    Science.gov (United States)

    Watanabe, T; Aonuma, H

    2014-02-01

    Hu/ELAV-like RNA-binding proteins (RBPs) are involved in the post-transcriptional regulation of RNA metabolism including splicing, transport, translational control and turnover. The Hu/ELAV-like RBP genes are predominantly expressed in neurons, and are therefore used as common neuronal markers in many animals. Although the expression patterns and functions of the Hu/ELAV-like RBP genes have been extensively studied in the model insect Drosophila melanogaster, little is known in basal direct-developing insects. In the present study, we performed an identification and expression analysis of the found in neurons (fne) gene, an ancestral insect Hu/ELAV-like RBP gene, in the cricket Gryllus bimaculatus. Contrary to expectation that the Gryllus fne transcript would be predominantly expressed in the nervous system, expression analysis revealed that the Gryllus fne gene is expressed broadly. In addition, we discovered that alternative promoter usage directs tissue-specific and embryonic stage-dependent regulation of fne expression, and that alternative splicing contributes to the generation of diverse sets of fne transcripts. Our data provide novel insights into the evolutionary diversification of the Hu/ELAV-like RBP gene family in insects. © 2013 The Royal Entomological Society.

  18. HU Sirong distinguishes key points of treating cor pulmonale heart failure%胡思荣辨治肺心病心衰

    Institute of Scientific and Technical Information of China (English)

    左明晏

    2016-01-01

    胡思荣教授从事中、西医临床及科研工作40余年,积累了丰富的医疗经验。胡师精于仲景学说,兼通温病各家诸说,擅长运用相关理论辨治肺心病心衰。提倡从痰饮论治肺心病心衰,认为肺心病阳虚为本,痰饮为基本病因,以温化痰饮为主--务,灵活运用温药除其湿,采用中病既止之法则。%Professor HU Sirong engaged in clinical and scientific research between Chinese and western medicine more than 40 years,has accumulated rich experience in health care. Hu teacher skilled in zhang zhongjing theory,and warm disease all said,are good at using relevant theory of treating cor pulmonale heart failure. Advocates from phlegm retention differentiation of cor pulmonale heart failure,thought that Yang deficiency and phlegm retention as the basic etiology,phlegm drink warm as the main priority,and flexible use of medicine in addition to the wet warm,adopt the rules of disease in both check.

  19. El zorro Andino, P seudalopes P seudalopes P culpaeus , un nuevo huésped para Corynosoma obtuscens (Acanthocephala en el Perú*

    Directory of Open Access Journals (Sweden)

    Manuel Tantaleán

    2013-04-01

    Full Text Available Se describe la presencia de Corynosoma obtuscens Lincicome, 1943 (Acanthocephala, Polymor- Corynosoma obtuscens L i n c i c o m e , 1 9 4 3 ( A c a n t h o c e p h a l a , P o l y m o r - Corynosoma obtuscens phidae en el intestino delgado del zorro andino Pseudalopex culpaeus (Molina, 1782 procedente Pseudalopex culpaeus ( M o l i n a , 1 7 8 2 p r o c e d e n t e Pseudalopex culpaeus de San Juan de Marcona, Departamento de Ica, Perú. Esta es la segunda vez que se registra la infección natural de un mamífero terrestre por C. obtuscens en el Perú, por lo que se considera C. obtuscens en el Perú, por lo que se considera C. obtuscens a P. culpaeus como huésped defi nitivo accidental P. culpaeus como huésped defi nitivo accidental P. culpaeus ; además se comenta la posibilidad de que éste acantocefalo infecte al hombre.

  20. Spatio-Temporal Patterns of Urban-Rural Development and Transformation in East of the “Hu Huanyong Line”, China

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    Zhichao Hu

    2016-02-01

    Full Text Available Urban-rural development and transformation is profoundly changing the socioeconomic system as well as the natural environment. The study uses the AHP (Analytic Hierarchy Process method to construct a top-down index of human activity based around five dimensions (population, land, industry, society, and environment to evaluate the spatial characteristics in the region east of the Hu Huanyong line, China, in 1994 and 2010. Then, we investigate the spatial-temporal pattern using the methods of hotspot analysis, local Moran’s I index and Pearson correlation coefficient. The calculation showed that: (1 northeast China was experiencing an economic recession during study period, and the implementation of revitalization plan have not controlled the recession trend yet; (2 Pearson correlation analysis showed that the improvement of population quality promote the development of industry and society systems significantly during study period; and (3 negative correlation between Population Development Index (PDI change and Population Transformation Index (PTI change (along with the Society Transformation Index (STI change and Industry Transformation Index (ITI change reflected that east of the Hu Huanyong line, China was in a “demographic dividend” period. Then, with the help of SOFM neural network algorithm, we divided the study area into six types of region, and found that municipalities, provincial capitals, Yangtze River Delta region and cities on the North China Plain owned the greatest development, while cities in southwest and northeast China showed relatively poor development during study period.