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Sample records for hu phys rev

  1. Comments on Phys. Rev. D89 (2014) 097101 "Reevaluation of the parton distribution of strange quarks in the nucleon"

    CERN Document Server

    Stolarski, M

    2014-01-01

    The HERMES collaboration in Phys. Rev. D89 (2014) 097101 extracted information about the strange quark density in the nucleon. One of the main results is an observation that the shape of the extracted density is very different from the shapes of the strange quark density from global QCD fits and also from that of the light antiquarks. In this paper systematic studies on the HERMES published multiplicity of pion and kaon data are presented. It is shown that the conclusions concerning the strange quark distribution in the nucleon reached in Phys. Rev. D89 (2014) 097101 are at the moment premature.

  2. Reply Comments on Phys. Rev. D89, 097101 (2014) "Reevaluation of the parton distribution of strange quarks in the nucleon"

    CERN Document Server

    Aschenauer, E C; Joosten, S; Rith, K; Schnell, G; Van Hulse, C

    2015-01-01

    In the "Comments on Phys. Rev. D89, 097101 (2014)", the Author presents a number of studies to conjecture that the analysis by the HERMES Collaboration presented in Phys. Rev. D89, 097101 (2014) likely suffers from effects that invalidate the leading-order analysis used in that publication. In our opinion, the Author has drawn erroneous conclusions. We present below a discussion of his arguments and the results from a repetition of that analysis using a range of parton distribution sets. The spread in those results precludes credible conclusions and demonstrates the sensitivity of the analysis to poorly known input data (unfavored FFs, strange-quark distributions, and mixed singlet and nonsinglet quantities).

  3. Comment on "Total Negative Refraction in Real Crystals for Ballistic Electrons and Light" (Phys. Rev. Lett. 91, 157404 (2003))

    OpenAIRE

    Yau, H. -F.; Liu, J.-P.; Ke, B; Kuo, C. -H.; Ye, Z.

    2003-01-01

    Recently, Zhang et al. (Phys. Rev. Lett. 91, 157404 (2003)) have demonstrated that an amphoteric refraction, i. e. both positive and negative refraction, may prevail at the interface of two uniaxial anisotropic crystals when their optical axes are in different directions. The authors subsequently made a correspondence between such a refraction with the negative refraction expected for Left Handed Materials (LHMs). Here we comment that the amphoteric refraction can be observed even with one un...

  4. Comments on Letter (Phys. Rev. L, Vol.89, No. 10,2002) by D. Shapira and M. Saltmarsh

    CERN Document Server

    Taleyarkhan, Rusi P; Cho, JaeSeon; Lahey, Richard T; Nigmatulin, Robert I; Block, Robert C

    2013-01-01

    This article focuses on correcting several factual errors and critiques in the previously published Letter in Phys. Rev. L, Vol. 89, No. 10, 2022, by D. Shapira and M. Saltmarsh. The authors of the Letter did not perform their own "independent" experiments as claimed; they did not perform control experiments with normal acetone; and, neither did they monitor for tritium. It their Letter, the authors (D. Shapira and M. Saltmarsh) failed to disclose that the data they collected actually confirmed our claims of having observed statistically significant nuclear emissions in chilled, cavitated deuterated acetone.

  5. Comment on “Diffusion of n-type dopants in germanium” [Appl. Phys. Rev. 1, 011301 (2014)

    Energy Technology Data Exchange (ETDEWEB)

    Cowern, N. E. B., E-mail: nick.cowern@ncl.ac.uk; Simdyankin, S.; Goss, J. P. [School of Electrical and Electronic Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU (United Kingdom); Napolitani, E.; De Salvador, D. [CNR-IMM-MATIS and Dipartimento di Fisica, Università di Padova, Via Marzolo 8, 35131 Padova (Italy); Bruno, E.; Mirabella, S. [CNR-IMM-MATIS and Dipartimento di Fisica e Astronomia, Università di Catania, Via S. Sofia 64, 95123 Catania (Italy); Ahn, C. [Samsung Semiconductor, Inc., 3655 North First Street, San Jose, California 95134 (United States); Bennett, N. S. [Institute of Mechanical, Process and Energy Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom)

    2015-09-15

    The authors of the above paper call into question recent evidence on the properties of self-interstitials, I, in Ge [Cowern et al., Phys. Rev. Lett. 110, 155501 (2013)]. We show that this judgment stems from invalid model assumptions during analysis of data on B marker-layer diffusion during proton irradiation, and that a corrected analysis fully supports the reported evidence. As previously stated, I-mediated self-diffusion in Ge exhibits two distinct regimes of temperature, T: high-T, dominated by amorphous-like mono-interstitial clusters—i-morphs—with self-diffusion entropy ≈30 k, and low-T, where transport is dominated by simple self-interstitials. In a transitional range centered on 475 °C both mechanisms contribute. The experimental I migration energy of 1.84 ± 0.26 eV reported by the Münster group based on measurements of self-diffusion during irradiation at 550 °C < T < 680 °C further establishes our proposed i-morph mechanism.

  6. Erratum: Measurement of transverse single-spin asymmetries for J/psi production in polarized p+p collisions at sqrt(s)=200 GeV [Phys. Rev. D 82, 112008 (2010)

    CERN Document Server

    Adare, A; Aidala, C; Ajitanand, N N; Akiba, Y; Al-Bataineh, H; Alexander, J; Al-Ta'ani, H; Angerami, A; Aoki, K; Apadula, N; Aphecetche, L; Aramaki, Y; Asai, J; Atomssa, E T; Averbeck, R; Awes, T C; Azmoun, B; Babintsev, V; Bai, M; Baksay, G; Baksay, L; Baldisseri, A; Barish, K N; Barnes, P D; Bassalleck, B; Basye, A T; Bathe, S; Batsouli, S; Baublis, V; Baumann, C; Bazilevsky, A; Belikov, S; Belmont, R; Bennett, R; Berdnikov, A; Berdnikov, Y; Bhom, J H; Bickley, A A; Blau, D S; Boissevain, J G; Bok, J S; Borel, H; Borggren, N; Boyle, K; Brooks, M L; Buesching, H; Bumazhnov, V; Bunce, G; Butsyk, S; Camacho, C M; Campbell, S; Caringi, A; Chang, B S; Chang, W C; Charvet, J -L; Chen, C -H; Chernichenko, S; Chi, C Y; Chiu, M; Choi, I J; Choi, J B; Choudhury, R K; Christiansen, P; Chujo, T; Chung, P; Churyn, A; Chvala, O; Cianciolo, V; Citron, Z; Cole, B A; del Valle, Z Conesa; Connors, M; Constantin, P; Csanád, M; Csörgö, T; Dahms, T; Dairaku, S; Danchev, I; Das, K; Datta, A; David, G; Dayananda, M K; Denisov, A; d'Enterria, D; Deshpande, A; Desmond, E J; Dharmawardane, K V; Dietzsch, O; Dion, A; Donadelli, M; Orazio, L D; Drapier, O; Drees, A; Drees, K A; Dubey, A K; Durham, J M; Durum, A; Dutta, D; Dzhordzhadze, V; Edwards, S; Efremenko, Y V; Ellinghaus, F; Engelmore, T; Enokizono, A; En'yo, H; Esumi, S; Eyser, K O; Fadem, B; Fields, D E; Finger,, M; Finger, M; Fleuret, F; Fokin, S L; Fraenkel, Z; Frantz, J E; Franz, A; Frawley, A D; Fujiwara, K; Fukao, Y; Fusayasu, T; Garishvili, I; Glenn, A; Gong, H; Gonin, M; Gosset, J; Goto, Y; de Cassagnac, R Granier; Grau, N; Greene, S V; Grim, G; Perdekamp, M Grosse; Gunji, T; Gustafsson, H -Å; Henni, A Hadj; Haggerty, J S; Hahn, K I; Hamagaki, H; Hamblen, J; Hanks, J; Han, R; Hartouni, E P; Haruna, K; Haslum, E; Hayano, R; Heffner, M; Hemmick, T K; Hester, T; He, X; Hill, J C; Hohlmann, M; Holzmann, W; Homma, K; Hong, B; Horaguchi, T; Hornback, D; Huang, S; Ichihara, T; Ichimiya, R; Iinuma, H; Ikeda, Y; Imai, K; Imrek, J; Inaba, M; Isenhower, D; Ishihara, M; Isobe, T; Issah, M; Isupov, A; Ivanischev, D; Iwanaga, Y; Jia, B V Jacak J; Jiang, X; Jin, J; Johnson, B M; Jones, T; Joo, K S; Jouan, D; Jumper, D S; Kajihara, F; Kametani, S; Kamihara, N; Kamin, J; Kang, J H; Kapustinsky, J; Karatsu, K; Kasai, M; Kawall, D; Kawashima, M; Kazantsev, A V; Kempel, T; Khanzadeev, A; Kijima, K M; Kikuchi, J; Kim, A; Kim, B I; Kim, D H; Kim, D J; Kim, E J; Kim, E; Kim, S H; Kim, Y -J; Kinney, E; Kiriluk, K; Kiss, Á; Kistenev, E; Klay, J; Klein-Boesing, C; Kochenda, L; Komkov, B; Konno, M; Koster, J; Kozlov, A; Král, A; Kravitz, A; Kunde, G J; Kurita, K; Kurosawa, M; Kweon, M J; Kwon, Y; Kyle, G S; Lacey, R; Lai, Y S; Lajoie, J G; Layton, D; Lebedev, A; Lee, D M; Lee, J; Lee, K B; Lee, K S; Lee, T; Leitch, M J; Leite, M A L; Lenzi, B; Lichtenwalner, P; Liebing, P; Levy, L A Linden; Liška, T; Litvinenko, A; Liu, H; Liu, M X; Li, X; Love, B; Lynch, D; Maguire, C F; Makdisi, Y I; Malakhov, A; Malik, M D; Manko, V I; Mannel, E; Mao, Y; Mašek, L; Masui, H; Matathias, F; McCumber, M; McGaughey, P L; Means, N; Meredith, B; Miake, Y; Mibe, T; Mignerey, A C; Mikeš, P; Miki, K; Milov, A; Mishra, M; Mitchell, J T; Mohanty, A K; Moon, H J; Morino, Y; Morreale, A; Morrison, D P; Moukhanova, T V; Mukhopadhyay, D; Murakami, T; Murata, J; Nagamiya, S; Nagle, J L; Naglis, M; Nagy, M I; Nakagawa, I; Nakamiya, Y; Nakamura, K R; Nakamura, T; Nakano, K; Nam, S; Newby, J; Nguyen, M; Nihashi, M; Niita, T; Nouicer, R; Nyanin, A S; Oakley, C; O'Brien, E; Oda, S X; Ogilvie, C A; Okada, K; Oka, M; Onuki, Y; Oskarsson, A; Ouchida, M; Ozawa, K; Pak, R; Palounek, A P T; Pantuev, V; Papavassiliou, V; Park, I H; Park, J; Park, S K; Park, W J; Pate, S F; Pei, H; Peng, J -C; Pereira, H; Peresedov, V; Peressounko, D Yu; Petti, R; Pinkenburg, C; Pisani, R P; Proissl, M; Purschke, M L; Purwar, A K; Qu, H; Rak, J; Rakotozafindrabe, A; Ravinovich, I; Read, K F; Rembeczki, S; Reygers, K; Riabov, V; Riabov, Y; Richardson, E; Roach, D; Roche, G; Rolnick, S D; Rosati, M; Rosen, C A; Rosendahl, S S E; Rosnet, P; Rukoyatkin, P; Ružička, P; Rykov, V L; Sahlmueller, B; Saito, N; Sakaguchi, T; Sakai, S; Sakashita, K; Samsonov, V; Sano, S; Sato, T; Sawada, S; Sedgwick, K; Seele, J; Seidl, R; Semenov, A Yu; Semenov, V; Seto, R; Sharma, D; Shein, I; Shibata, T -A; Shigaki, K; Shimomura, M; Shoji, K; Shukla, P; Sickles, A; Silva, C L; Silvermyr, D; Silvestre, C; Sim, K S; Singh, B K; Singh, C P; Singh, V; Slunečka, M; Soldatov, A; Soltz, R A; Sondheim, W E; Sorensen, S P; Sourikova, I V; Staley, F; Stankus, P W; Stenlund, E; Stepanov, M; Ster, A; Stoll, S P; Sugitate, T; Suire, C; Sukhanov, A; Sziklai, J; Takagui, E M; Taketani, A; Tanabe, R; Tanaka, Y; Taneja, S; Tanida, K; Tannenbaum, M J; Tarafdar, S; Taranenko, A; Tarján, P; Themann, H; Thomas, D; Thomas, T L; Togawa, M; Toia, A; Tomášek, L; Tomita, Y; Torii, H; Towell, R S

    2012-01-01

    We previously reported [Phys. Rev. D 82, 112008 (2010)] measurements of transverse single-spin asymmetries, A_N, in J/psi production from transversely polarized p+p collisions at sqrt(s)=200 GeV with data taken by the PHENIX experiment at the Relativistic Heavy Ion Collider in 2006 and 2008. Subsequently, we have found errors in the analysis procedures for the 2008 data, which resulted in an erroneous value for the extracted A_N. The errors affected the sorting of events into the correct left/right and forward/backward bins. This produced an incorrect value for the 2008 result, but the 2006 result is unaffected. We have conducted two independent reanalyses with these errors corrected, and we present here the corrected values for the 2008 data and the combined results for 2006 and 2008. The new combined spin asymmetry in the forward region is A_N = -0.026+/-0.026(stat)+/-0.003(sys). Since this asymmetry is consistent with zero, we no longer claim that our results suggest a possible non-zero trigluon correlatio...

  7. Retraction: On the origin of power-law distributions in systems with constrained phase space [Condens. Matter Phys., 2013, vol. 16, 43802

    Directory of Open Access Journals (Sweden)

    Editorial Board

    2014-03-01

    Full Text Available The article Condens. Matter Phys., 2013, vol. 16, 43802 ( DOI:10.5488/CMP.16.43802 has been retracted by the decision of the Editorial Board. There is a significant overlap with an article: Phys. Rev. E, 2006, vol. 74, 036120 ( DOI:10.1103/PhysRevE.74.036120. Appologies are offered to readers of the journal that this was not detected during the submission process.

  8. Expanding the PhysTEC Coalition

    Science.gov (United States)

    Stein, Fredrick

    2003-04-01

    The Physics Teacher Education Coalition (PhysTEC) is a community of physics departments representing scientists and educators at institutions dedicated to improving the science preparation of future K-12 teachers. Now in its second year, PhysTEC requires physics and education faculty to work together to provide an education for future teachers that emphasizes interactive engagement and a student-centered approach to learning science. The first six Coalition members are the physics departments at Ball State University, Oregon State University, University of Arizona, University of Arkansas, Western Michigan University, and Xavier University of Louisiana. PhysTEC is creating a broad, active Coalition of physics departments that have implemented or are interested in implementing one or more of the PhysTEC Program Components. · A long-term, active collaboration among the physics department, the department of education, and the local schools. · A Teacher-in-Residence (TIR) program that provides for a full-time participant in assisting faculty in course revisions. · The redesign of physics courses based on results from physics education research. · The redesign of elementary and secondary science methods courses with an emphasis on inquiry-based teaching and learning. · The establishment of a mentoring program to provide a valuable induction experience for novice science teachers. · The participation of physics faculty in the improvement and expansion of school experiences. www.phystec.org

  9. Main: 1HU9 [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1HU9 大豆 Soybean Glycine max (L.) Merrill Seed Lipoxygenase-3 Name=Lox1.3; Synonyms=...2; CAA31664.1; ALT_SEQ.|EMBL; U50081; AAB41272.1; -.|PDB; 1HU9; X-ray; A=1-857.|PDB; 1IK3; X-ray; A=1-857.|P...WTSDTRALEAFKRFGNKLAQIENKLSERNNDEKLRNRCGPVQMPYTLLLPSSKEGLTFRGIPNSISI soybean_1HU9.jpg ...

  10. Infecciones en huéspedes inmunocomprometidos

    OpenAIRE

    Cuéllar Ponce de León, Luis Ernesto; Médico-Cirujano. Especialista en Enfermedades Infecciosas y Tropicales. Jefe del Servicio de Infectología. Instituto Nacional de Enfermedades Neoplásicas. Coordinador del Programa VIH/SIDA/TARGA-INEN. Profesor Asociado de Medicina de la Universidad Nacional Federico Villarreal. Lima.

    2013-01-01

    Las infecciones son causa frecuente de morbimortalidad en huéspedes inmunocomprometidos. La frecuencia y diversidad de infecciones en esta población dependerá del tipo de inmunocompromiso, tratamiento recibido y aspecto epidemiológico. Los huéspedes con neutropenia desarrollan infecciones por bacterias extracelulares no encapsuladas y hongos levaduriformes y filamentosos; el diagnóstico precoz y tratamiento oportuno de las mismas son considerados una emergencia por su severidad y gran mortali...

  11. Erratum: Axion Dark Matter Coupling to Resonant Photons via Magnetic Field [Phys. Rev. Lett. 116, 161804 (2016)

    Science.gov (United States)

    McAllister, Ben T.; Parker, Stephen R.; Tobar, Michael E.

    2016-10-01

    A recent comment highlights a discussion at the PATRAS Workshop on Axions, WIMPs and WISPs relating to a recent publication. We respond to this comment, and detail a calculation error in the original letter.

  12. Erratum: Creation of X-Ray Transparency of Matter by Stimulated Elastic Forward Scattering [Phys. Rev. Lett. 115 , 107402 (2015)

    Energy Technology Data Exchange (ETDEWEB)

    Stöhr, J.; Scherz, A.

    2016-01-06

    X-ray absorption by matter has long been described by the famous Beer-Lambert law. Here we show how this fundamental law needs to be modified for high-intensity coherent x-ray pulses, now available at x-ray free electron lasers, due to the onset of stimulated elastic forward scattering. We present an analytical expression for the modified polarization-dependent Beer-Lambert law for the case of resonant core-to-valence electronic transitions and incident transform limited x-ray pulses. Upon transmission through a solid, the absorption and dichroic contrasts are found to vanish with increasing x-ray intensity, with the stimulation threshold lowered by orders of magnitude through a super-radiative coherent effect. Our results have broad implications for the study of matter with x-ray lasers.

  13. A probabilistic Hu-Washizu variational principle

    Science.gov (United States)

    Liu, W. K.; Belytschko, T.; Besterfield, G. H.

    1987-01-01

    A Probabilistic Hu-Washizu Variational Principle (PHWVP) for the Probabilistic Finite Element Method (PFEM) is presented. This formulation is developed for both linear and nonlinear elasticity. The PHWVP allows incorporation of the probabilistic distributions for the constitutive law, compatibility condition, equilibrium, domain and boundary conditions into the PFEM. Thus, a complete probabilistic analysis can be performed where all aspects of the problem are treated as random variables and/or fields. The Hu-Washizu variational formulation is available in many conventional finite element codes thereby enabling the straightforward inclusion of the probabilistic features into present codes.

  14. For Hu Zhuanglin: A Prefatory Note

    Institute of Scientific and Technical Information of China (English)

    M. A. K. Halliday

    2012-01-01

    It is a very great pleasure to be able to congratulate Professor Hu Zhuanglin on the occasion of his eightieth birthday. It is also a time to remember that Professor Hu and I have known each other for 33 years, since the moment he arrived in Sydney, together with eight other Chinese scholars, to take part in a programme of studies arranged jointly by the Department of English and the Department of Linguistics at Sydney University. These first arrivals from China were people of some seniority,

  15. Phonon Squeezing by Raman Scattering.

    Science.gov (United States)

    Nori, Franco; Hu, Xuedong

    1997-03-01

    We have studied quantum fluctuation properties of a crystal lattice, and proposed several mechanisms to achieve this goal(X. Hu and F. Nori, Phys. Rev. Lett. 76, 2294 (1996); Phys. Rev. B 53, 2419 (1996); preprint.).

  16. Hu on poolel teel / James Miles

    Index Scriptorium Estoniae

    Miles, James

    2006-01-01

    2007. aastal toimuv kommunistliku partei 17. kongress tähistab Hiina presidendi Hu Jintao jaoks oma võimuaja poole peale jõudmist, ees ootavad sellised väljakutsed nagu olümpiamängude korraldamine, valimiskeeris Taiwanis ning hullunud majanduse stabiliseerimine. Vt samas: Hiina avanemise pidu

  17. Hu on poolel teel / James Miles

    Index Scriptorium Estoniae

    Miles, James

    2006-01-01

    2007. aastal toimuv kommunistliku partei 17. kongress tähistab Hiina presidendi Hu Jintao jaoks oma võimuaja poole peale jõudmist, ees ootavad sellised väljakutsed nagu olümpiamängude korraldamine, valimiskeeris Taiwanis ning hullunud majanduse stabiliseerimine. Vt samas: Hiina avanemise pidu

  18. 12MW Horns Rev experiment

    DEFF Research Database (Denmark)

    Hasager, Charlotte Bay; Peña, A.; Mikkelsen, Torben

    The 12MW project with the full title ‘12 MW wind turbines: the scientific basis for their operation at 70 to 270 m height offshore’ has the goal to experimentally investigate the wind and turbulence characteristics between 70 and 270 m above sea level and thereby establish the scientific basis...... relevant for the next generation of huge 12 MW wind turbines operating offshore. The report describes the experimental campaign at the Horns Rev offshore wind farm at which observations from Doppler Laser LIDAR and SODAR were collected from 3 May to 24 October 2006. The challenges for mounting...... profile. Further studies on this part of the work are on-going. Technical detail on LIDAR and SODAR are provided as well as theoretical work on turbulence and atmospheric boundary layer flow. Selected results from the experimental campaign are reported....

  19. SUPPORT FOR HU CFRT SUMMER HIGH SCHOOL FUSION WORKSHOP

    Energy Technology Data Exchange (ETDEWEB)

    Punjabi, Alkesh

    2010-02-09

    researchers in the HU CFRT mentor the students during summers. Mentors spend a considerable amount of time and efforts in training, teaching, guiding and supervising research projects. The HU CFRT has so far conducted nine workshops during the summers of 1996-2000 and 2002-2005. The first workshop was conducted in summer 1996. Students for the workshop are chosen from a national pool of exceptionally talented high school rising seniors/juniors. To our knowledge, most of these students have gone on to prestigious universities such as Duke University, John Hopkins University, CalTech, UCLA, Hampton University, etc. after completing their high school. For instance, Tiffany Fisher, participant of the 1996 summer workshop completed her BS in Mathematics at Hampton University in May 2001. She then went on to Wake Forest University at Winston-Salem, North Carolina to pursue graduate studies. Anshul Haldipur, participant of the 1999 summer workshop, began his undergraduate studies at Duke University in 2000. Christina Nguyen and Ilissa Martinez, participants of the 2000 summer workshop, are pursuing their undergraduate degrees at the UCLA and Florida State University respectively. The organizing committee of the APS DPP annual meeting invited Dr. Punjabi to deliver an invited talk on training the next generation of fusion scientists and engineers at the 2005 APS DPP meeting in Denver, CO. The organizing committee distributed a special flier with the Bulletin to highlight this invited talk and another talk on education as well the expo. This has given wide publicity and recognition to our workshops and Hampton University. Prof. Punjabi's talk: 'LI2 2: Training the next generation of fusion scientists and engineers: summer high school fusion science workshop, Bull. Amer. Phys. Soc. 50, 221 (2005)' was very well-received. He talked about HU education and outreach initiative and the HU CFRT Summer High School Workshop. The audience had a considerable number of questions

  20. Hu Xiaoyu—an Outstanding Pupil

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    HU Xiaoyu, 11, is a fifth grader at the Beijing Hepingli No. 4 Primary School. She is talkative, cheerful, active and likes to laugh. Her teachers say she is well-behaved and excels in her studies. She is intelligent and always asks many questions both in and outside the classroom. She has been elected a "Three Good" student every year since she started school.

  1. Hu Qiaomu and Hu Feng Event%胡乔木与胡风案

    Institute of Scientific and Technical Information of China (English)

    张成洁

    2015-01-01

    It had a gradual upgrade process on a critique of Hu Feng's literary thoughts and a political settlement to Hu Feng.As the senior vice minister of the Propaganda Department of the Central Committee of the CPC,Hu Qiaomu played an important role on it.In October 1945,Hu Qiaomu still stayed in Chungking after Chungking Negotiations and counter-acted the effects of Hu Feng's literary thoughts by criticizing"subjectiveness".Public criticism to Hufeng launched by"popular literature series" was supported by Hu Qiaomuin in Hongkong in 1948.The first two years of the founding of new China,Hu Qiaomu talked with Hufeng for many times to solve his ideological problems.Hu Qiaomu also organized criticism of Hu Feng's literary thoughts throught the opportunity of Shuwu self-criticism;Hu Qiaomu first mentioned publicly"a small cabal of literary headed by Hufeng"In his giving editorial notes of Shuwu's article.Hu Feng's private let-ters to Shuwu were disclosed which directly led to Hu Feng event upgrading to"anti-party and anti-revolution"problems in the process of the great criticism of Hu Feng.After 1954,this matter had an indirect relationship with Hu Qiaomu's suggest of writing Hu Feng sectarianism.However,Hu Qiaomu disapproved to determine Hu Feng and the people impli-cating with Hu Feng as"Hufeng Anti-Party Clique".He gave a claim in this connection before Mao Zedong.In the past, people very paid attention to Zhouyang who had deep historical grievances with Hu Feng and ignored the role of Hu Qiao-mu in Hu Feng event.%对胡风文艺思想的批判和对胡风的政治清算有一个渐次升级的过程。其中,在对胡风文艺思想的批判中,胡乔木起了重要作用。1945年10月,胡乔木随毛泽东去重庆谈判后逗留重庆,借批《论主观》来清除胡风文艺思想的影响;1948年香港的《大众文艺丛刊》对胡风发起的公开批评,也得到了胡乔木的支持;新中国成立头两年,胡乔木多次奉命找胡风谈话

  2. Comment on ``Unified explanation of the anomalous dynamic properties of highly asymmetric polymer blends'' [J. Chem. Phys. 138, 054903 (2013)

    Science.gov (United States)

    Colmenero, J.

    2013-05-01

    In a recent paper by Ngai and Capaccioli ["Unified explanation of the anomalous dynamic properties of highly asymmetric polymer blends," J. Chem. Phys. 138, 054903 (2013), 10.1063/1.4789585] the authors claimed that the so-called coupling model (CM) provides a unified explanation of all dynamical anomalies that have been reported for dynamically asymmetric blends over last ten years. Approximately half of the paper is devoted to chain-dynamic properties involving un-entangled polymers. According to the authors, the application of the CM to these results is based on the existence of a crossover at a time tc ≈ 1-2 ns of the magnitudes describing chain-dynamics. Ngai and Capaccioli claimed that the existence of such a crossover is supported by the neutron scattering and MD-simulation results, corresponding to the blend poly(methyl methacrylate)/poly(ethylene oxide), by Niedzwiedz et al. [Phys. Rev. Lett. 98, 168301 (2007), 10.1103/PhysRevLett.98.168301] and Brodeck et al. [Macromolecules 43, 3036 (2010), 10.1021/ma902820a], respectively. Being one of the authors of these two papers, I will demonstrate here that there is no evidence supporting such a crossover in the data reported in these papers.

  3. SUPPORT FOR HU CFRT SUMMER HIGH SCHOOL FUSION WORKSHOP

    Energy Technology Data Exchange (ETDEWEB)

    Punjabi, Alkesh

    2010-02-09

    researchers in the HU CFRT mentor the students during summers. Mentors spend a considerable amount of time and efforts in training, teaching, guiding and supervising research projects. The HU CFRT has so far conducted nine workshops during the summers of 1996-2000 and 2002-2005. The first workshop was conducted in summer 1996. Students for the workshop are chosen from a national pool of exceptionally talented high school rising seniors/juniors. To our knowledge, most of these students have gone on to prestigious universities such as Duke University, John Hopkins University, CalTech, UCLA, Hampton University, etc. after completing their high school. For instance, Tiffany Fisher, participant of the 1996 summer workshop completed her BS in Mathematics at Hampton University in May 2001. She then went on to Wake Forest University at Winston-Salem, North Carolina to pursue graduate studies. Anshul Haldipur, participant of the 1999 summer workshop, began his undergraduate studies at Duke University in 2000. Christina Nguyen and Ilissa Martinez, participants of the 2000 summer workshop, are pursuing their undergraduate degrees at the UCLA and Florida State University respectively. The organizing committee of the APS DPP annual meeting invited Dr. Punjabi to deliver an invited talk on training the next generation of fusion scientists and engineers at the 2005 APS DPP meeting in Denver, CO. The organizing committee distributed a special flier with the Bulletin to highlight this invited talk and another talk on education as well the expo. This has given wide publicity and recognition to our workshops and Hampton University. Prof. Punjabi's talk: 'LI2 2: Training the next generation of fusion scientists and engineers: summer high school fusion science workshop, Bull. Amer. Phys. Soc. 50, 221 (2005)' was very well-received. He talked about HU education and outreach initiative and the HU CFRT Summer High School Workshop. The audience had a considerable number of questions

  4. Regulation of HuR by DNA Damage Response Kinases

    Directory of Open Access Journals (Sweden)

    Hyeon Ho Kim

    2010-01-01

    Full Text Available As many DNA-damaging conditions repress transcription, posttranscriptional processes critically influence gene expression during the genotoxic stress response. The RNA-binding protein HuR robustly influences gene expression following DNA damage. HuR function is controlled in two principal ways: (1 by mobilizing HuR from the nucleus to the cytoplasm, where it modulates the stability and translation of target mRNAs and (2 by altering its association with target mRNAs. Here, we review evidence that two main effectors of ataxia-telangiectasia-mutated/ATM- and Rad3-related (ATM/ATR, the checkpoint kinases Chk1 and Chk2, jointly influence HuR function. Chk1 affects HuR localization by phosphorylating (hence inactivating Cdk1, a kinase that phosphorylates HuR and thereby blocks HuR's cytoplasmic export. Chk2 modulates HuR binding to target mRNAs by phosphorylating HuR's RNA-recognition motifs (RRM1 and RRM2. We discuss how HuR phosphorylation by kinases including Chk1/Cdk1 and Chk2 impacts upon gene expression patterns, cell proliferation, and survival following genotoxic injury.

  5. Flexible DNA bending in HU-DNA cocrystal structures.

    Science.gov (United States)

    Swinger, Kerren K; Lemberg, Kathryn M; Zhang, Ying; Rice, Phoebe A

    2003-07-15

    HU and IHF are members of a family of prokaryotic proteins that interact with the DNA minor groove in a sequence-specific (IHF) or non-specific (HU) manner to induce and/or stabilize DNA bending. HU plays architectural roles in replication initiation, transcription regulation and site-specific recombination, and is associated with bacterial nucleoids. Cocrystal structures of Anabaena HU bound to DNA (1P71, 1P78, 1P51) reveal that while underlying proline intercalation and asymmetric charge neutralization mechanisms of DNA bending are similar for IHF and HU, HU stabilizes different DNA bend angles ( approximately 105-140 degrees ). The two bend angles within a single HU complex are not coplanar, and the resulting dihedral angle is consistent with negative supercoiling. Comparison of HU-DNA and IHF-DNA structures suggests that sharper bending is correlated with longer DNA binding sites and smaller dihedral angles. An HU-induced bend may be better modeled as a hinge, not a rigid bend. The ability to induce or stabilize varying bend angles is consistent with HU's role as an architectural cofactor in many different systems that may require differing geometries.

  6. HU-446 and HU-465, Derivatives of the Non-psychoactive Cannabinoid Cannabidiol, Decrease the Activation of Encephalitogenic T Cells.

    Science.gov (United States)

    Kozela, Ewa; Haj, Christeene; Hanuš, Lumir; Chourasia, Mukesh; Shurki, Avital; Juknat, Ana; Kaushansky, Nathali; Mechoulam, Raphael; Vogel, Zvi

    2016-01-01

    Cannabidiol (CBD), the non-psychoactive cannabinoid, has been previously shown by us to decrease peripheral inflammation and neuroinflammation in mouse experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS). Here we have studied the anti-inflammatory effects of newly synthesized derivatives of natural (-)-CBD ((-)-8,9-dihydro-7-hydroxy-CBD; HU-446) and of synthetic (+)-CBD ((+)-8,9-dihydro-7-hydroxy-CBD; HU-465) on activated myelin oligodendrocyte glycoprotein (MOG)35-55-specific mouse encephalitogenic T cells (T(MOG) ) driving EAE/MS-like pathologies. Binding assays followed by molecular modeling revealed that HU-446 has negligible affinity toward the cannabinoid CB1 and CB2 receptors while HU-465 binds to both CB1 and CB2 receptors at the high nanomolar concentrations (Ki = 76.7 ± 5.8 nm and 12.1 ± 2.3 nm, respectively). Both, HU-446 and HU-465, at 5 and 10 μm (but not at 0.1 and 1 μm), inhibited the MOG35-55-induced proliferation of autoreactive T(MOG) cells via CB1/CB2 receptor independent mechanisms. Moreover, both HU-446 and HU-465, at 5 and 10 μm, inhibited the release of IL-17, a key autoimmune cytokine, from MOG35-55-stimulated T(MOG) cells. These results suggest that HU-446 and HU-465 have anti-inflammatory potential in inflammatory and autoimmune diseases.

  7. Multiple defects in Escherichia coli mutants lacking HU protein.

    OpenAIRE

    Huisman, O; Faelen, M; Girard, D; Jaffé, A; Toussaint, A; Rouvière-Yaniv, J

    1989-01-01

    The HU protein isolated from Escherichia coli, composed of two partially homologous subunits, alpha and beta, shares some of the properties of eucaryotic histones and is a major constituent of the bacterial nucleoid. We report here the construction of double mutants totally lacking both subunits of HU protein. These mutants exhibited poor growth and a perturbation of cell division, resulting in the formation of anucleate cells. In the absence of HU, phage Mu was unable to grow, to lysogenize,...

  8. A Working Day of Professor Hu Yamei

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    Professor Hu Yamei is a well-known pediatrician and one of the first group of academicians to the Institute of Medical Science attached to the Chinese Academy of Engineering. At the start of her career in the 1950s, she focused on nutritional anemia that afflicts children, developing treatment and methods of prevention. Later she researched etiology, pathology and physiology of pediatric toxic indigestion, helping to reduce the child death rate from 20 percent to 1 percent. Over the past 20 years, she has concentrated on pediatric acute lymphoblastic leukemia. As a result, the five-years living rate of the disease treated by Hu’s group has reached 74 percent, approaching the highest level in the world.

  9. Notas sobre el paisaje de Huánuco Pampa

    OpenAIRE

    Román Godines, Oscar; Ministerio de Cultura

    2013-01-01

    El complejo arqueológico Huánuco Pampa se encuentra ubicado en la provincia Dos de Mayo en el departamento de Huánuco y en la actualidad es quizás el principal vestigio de la cosmovisión y la planificación urbana inca.

  10. Rapid purification of HU protein from Halobacillus karajensis

    Directory of Open Access Journals (Sweden)

    Parinaz Ghadam

    2014-03-01

    Full Text Available The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to cruciform DNA and repair intermediates such as nick, gap, bulge, etc. To understand the features of HU binding to DNA and repair intermediates, a fast and easy HU protein purification method is required. Here we report a two-step purification procedure of HU from Halobacillus karajensis (the gram positive and moderately halophilic bacteria isolated from Karaj surface soil. The method of HU purification allows obtaining a pure non-tagged protein. Salting out and ion exchange chromatography were applied for purification, and the purified protein was identified by immunoblotting. Results showed that the molecular weight of the purified protein was approximately 11 kDa which is immunologically similar to the Bacillus subtilis HU protein (HBsu.

  11. Research-based resources on PhysPort

    Science.gov (United States)

    Sayre, Eleanor

    2017-01-01

    PhysPort (http://physport.org) is a website that supports physics faculty in implementing research-based teaching practices in their classrooms. We provide expert recommendations and practical information about teaching methods and assessment. The PhysPort Data Explorer is an intuitive online tool for physics faculty to analyze their assessment data. Faculty upload their students' responses using our secure interface. The Data Explorer matches their pre/post data, scores it, compares it to national data, and graphs it in an interactive and intuitive manner. The Periscope collection on Physport brings together classroom video of students working groups with professional development materials for faculty, pre-service teachers, and learning assistants. To support PhysPort's development efforts, we conduct research on faculty needs around teaching and assessment, secondary analysis of published PER studies, and primary analysis of assessment data. In this talk, I'll introduce some of PhysPort's research-based resources and the research results which support them.

  12. Rev Variation during Persistent Lentivirus Infection

    Directory of Open Access Journals (Sweden)

    Karin S. Dorman

    2011-01-01

    Full Text Available The ability of lentiviruses to continually evolve and escape immune control is the central impediment in developing an effective vaccine for HIV-1 and other lentiviruses. Equine infectious anemia virus (EIAV is considered a useful model for immune control of lentivirus infection. Virus-specific cytotoxic T lymphocytes (CTL and broadly neutralizing antibody effectively control EIAV replication during inapparent stages of disease, but after years of low-level replication, the virus is still able to produce evasion genotypes that lead to late re-emergence of disease. There is a high rate of genetic variation in the EIAV surface envelope glycoprotein (SU and in the region of the transmembrane protein (TM overlapped by the major exon of Rev. This review examines genetic and phenotypic variation in Rev during EIAV disease and a possible role for Rev in immune evasion and virus persistence.

  13. HIV Rev Assembly on the Rev Response Element (RRE: A Structural Perspective

    Directory of Open Access Journals (Sweden)

    Jason W. Rausch

    2015-06-01

    Full Text Available HIV-1 Rev is an ~13 kD accessory protein expressed during the early stage of virus replication. After translation, Rev enters the nucleus and binds the Rev response element (RRE, a ~350 nucleotide, highly structured element embedded in the env gene in unspliced and singly spliced viral RNA transcripts. Rev-RNA assemblies subsequently recruit Crm1 and other cellular proteins to form larger complexes that are exported from the nucleus. Once in the cytoplasm, the complexes dissociate and unspliced and singly-spliced viral RNAs are packaged into nascent virions or translated into viral structural proteins and enzymes, respectively. Rev binding to the RRE is a complex process, as multiple copies of the protein assemble on the RNA in a coordinated fashion via a series of Rev-Rev and Rev-RNA interactions. Our understanding of the nature of these interactions has been greatly advanced by recent studies using X-ray crystallography, small angle X-ray scattering (SAXS and single particle electron microscopy as well as biochemical and genetic methodologies. These advances are discussed in detail in this review, along with perspectives on development of antiviral therapies targeting the HIV-1 RRE.

  14. Sparks sädeleb Rabarocki öös. Ettevaatust! Eliidi rünnak. Hu? Hu da hell?

    Index Scriptorium Estoniae

    2007-01-01

    Ameerika ansamblist Sparks. Vaiko Eplik & Eliit heliplaatidest "1: Aastaajad", "2: Aastaajad" ja "3: Kosmoseodüsseia", esinemisest festivalil Rabarock. Hannaliisa Uusma, Leslie Da Bassi ja DJ Criticali projektist HU?, singlitest "Kosmos" ja "Absoluutselt" (Rabarockil esitusel materjal HU? debüütalbumilt "Film")

  15. Sparks sädeleb Rabarocki öös. Ettevaatust! Eliidi rünnak. Hu? Hu da hell?

    Index Scriptorium Estoniae

    2007-01-01

    Ameerika ansamblist Sparks. Vaiko Eplik & Eliit heliplaatidest "1: Aastaajad", "2: Aastaajad" ja "3: Kosmoseodüsseia", esinemisest festivalil Rabarock. Hannaliisa Uusma, Leslie Da Bassi ja DJ Criticali projektist HU?, singlitest "Kosmos" ja "Absoluutselt" (Rabarockil esitusel materjal HU? debüütalbumilt "Film")

  16. Revised Safety Instruction 4 (IS4 REV.)

    CERN Multimedia

    2006-01-01

    Please note that the revised safety instruction 4 (IS4 REV.) entitled 'Safety Inspections' is available on the web at the following url: https://edms.cern.ch/file/335741/LAST_RELEASED/E_IS4.pdf Paper copies can also be obtained from the SC unit secretariat, e-mail: sc.secretariat@cern.ch SC Secretariat

  17. Thermodynamics of Rev-RNA interactions in HIV-1 Rev-RRE assembly.

    Science.gov (United States)

    Jayaraman, Bhargavi; Mavor, David; Gross, John D; Frankel, Alan D

    2015-10-27

    The HIV-1 protein Rev facilitates the nuclear export of intron-containing viral mRNAs by recognizing a structured RNA site, the Rev-response-element (RRE), contained in an intron. Rev assembles as a homo-oligomer on the RRE using its α-helical arginine-rich-motif (ARM) for RNA recognition. One unique feature of this assembly is the repeated use of the ARM from individual Rev subunits to contact distinct parts of the RRE in different binding modes. How the individual interactions differ and how they contribute toward forming a functional complex is poorly understood. Here we examine the thermodynamics of Rev-ARM peptide binding to two sites, RRE stem IIB, the high-affinity site that nucleates Rev assembly, and stem IA, a potential intermediate site during assembly, using NMR spectroscopy and isothermal titration calorimetry (ITC). NMR data indicate that the Rev-IIB complex forms a stable interface, whereas the Rev-IA interface is highly dynamic. ITC studies show that both interactions are enthalpy-driven, with binding to IIB being 20-30 fold tighter than to IA. Salt-dependent decreases in affinity were similar at both sites and predominantly enthalpic in nature, reflecting the roles of electrostatic interactions with arginines. However, the two interactions display strikingly different partitioning between enthalpy and entropy components, correlating well with the NMR observations. Our results illustrate how the variation in binding modes to different RRE target sites may influence the stability or order of Rev-RRE assembly and disassembly, and consequently its function.

  18. Testing the planetary models of HU Aquarii

    CERN Document Server

    Bours, M; Breedt, E; Copperwheat, C; Dhillon, V; Leckngam, A; Littlefair, S; Parsons, S; Prasit, A

    2014-01-01

    We present new eclipse observations of the polar (i.e. semi-detached magnetic white dwarf + M-dwarf binary) HU Aqr, and mid-egress times for each eclipse, which continue to be observed increasingly early. Recent eclipses occurred more than 70 seconds earlier than the prediction from the latest model that invoked a single circumbinary planet to explain the observed orbital period variations, thereby conclusively proving this model to be incorrect. Using ULTRACAM data, we show that mid-egress times determined for simultaneous data taken at different wavelengths agree with each other. The large variations in the observed eclipse times cannot be explained by planetary models containing up to three planets, because of poor fits to the data as well as orbital instability on short time scales. The peak-to-peak amplitude of the O-C diagram of almost 140 seconds is also too great to be caused by Applegate's mechanism, movement of the accretion spot on the surface of the white dwarf, or by asynchronous rotation of the ...

  19. CB2 cannabinoid receptor agonist enantiomers HU-433 and HU-308: An inverse relationship between binding affinity and biological potency.

    Science.gov (United States)

    Smoum, Reem; Baraghithy, Saja; Chourasia, Mukesh; Breuer, Aviva; Mussai, Naama; Attar-Namdar, Malka; Kogan, Natalya M; Raphael, Bitya; Bolognini, Daniele; Cascio, Maria G; Marini, Pietro; Pertwee, Roger G; Shurki, Avital; Mechoulam, Raphael; Bab, Itai

    2015-07-14

    Activation of the CB2 receptor is apparently an endogenous protective mechanism. Thus, it restrains inflammation and protects the skeleton against age-related bone loss. However, the endogenous cannabinoids, as well as Δ(9)-tetrahydrocannabinol, the main plant psychoactive constituent, activate both cannabinoid receptors, CB1 and CB2. HU-308 was among the first synthetic, selective CB2 agonists. HU-308 is antiosteoporotic and antiinflammatory. Here we show that the HU-308 enantiomer, designated HU-433, is 3-4 orders of magnitude more potent in osteoblast proliferation and osteoclast differentiation culture systems, as well as in mouse models, for the rescue of ovariectomy-induced bone loss and ear inflammation. HU-433 retains the HU-308 specificity for CB2, as shown by its failure to bind to the CB1 cannabinoid receptor, and has no activity in CB2-deficient cells and animals. Surprisingly, the CB2 binding affinity of HU-433 in terms of [(3)H]CP55,940 displacement and its effect on [(35)S]GTPγS accumulation is substantially lower compared with HU-308. A molecular-modeling analysis suggests that HU-433 and -308 have two different binding conformations within CB2, with one of them possibly responsible for the affinity difference, involving [(35)S]GTPγS and cAMP synthesis. Hence, different ligands may have different orientations relative to the same binding site. This situation questions the usefulness of universal radioligands for comparative binding studies. Moreover, orientation-targeted ligands have promising potential for the pharmacological activation of distinct processes.

  20. Quicklook overview of model changes in Melcor 2.2: Rev 6342 to Rev 9496

    Energy Technology Data Exchange (ETDEWEB)

    Humphries, Larry L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-05-01

    MELCOR 2.2 is a significant official release of the MELCOR code with many new models and model improvements. This report provides the code user with a quick review and characterization of new models added, changes to existing models, the effect of code changes during this code development cycle (rev 6342 to rev 9496), a preview of validation results with this code version. More detailed information is found in the code Subversion logs as well as the User Guide and Reference Manuals.

  1. On the HU Aquarii planetary system hypothesis

    Science.gov (United States)

    Goździewski, Krzysztof; Nasiroglu, Ilham; Słowikowska, Aga; Beuermann, Klaus; Kanbach, Gottfried; Gauza, Bartosz; Maciejewski, Andrzej J.; Schwarz, Robert; Schwope, Axel D.; Hinse, Tobias C.; Haghighipour, Nader; Burwitz, Vadim; Słonina, Mariusz; Rau, Arne

    2012-09-01

    In this paper, we investigate the eclipse timing of the polar binary HU Aquarii that has been observed for almost two decades. Recently, Qian et al. attributed large (O-C) deviations between the eclipse ephemeris and observations to a compact system of two massive Jovian companions. We improve the Keplerian, kinematic model of the light travel time effect and re-analyse the whole currently available data set. We add almost 60 new, yet unpublished, mostly precision light curves obtained using the time high-resolution photopolarimeter Optical Timing Analyzer (OPTIMA), as well as photometric observations performed at the Monitoring Network of Telescopes/North, Physics Innovations Robotic Astronomical Telescope Explorer and Carlos Sánchez Telescope. We determine new mid-egress times with a mean uncertainty at the level of 1 s or better. We claim that because the observations that currently exist in the literature are non-homogeneous with respect to spectral windows (ultraviolet, X-ray, visual and polarimetric mode) and the reported mid-egress measurements errors, they may introduce systematics that affect orbital fits. Indeed, we find that the published data, when taken literally, cannot be explained by any unique solution. Many qualitatively different and best-fit two-planet configurations, including self-consistent, Newtonian N-body solutions may be able to explain the data. However, using high-resolution, precision OPTIMA light curves, we find that the (O-C) deviations are best explained by the presence of a single circumbinary companion orbiting at a distance of ˜4.5 au with a small eccentricity and having ˜7 Jupiter masses. This object could be the next circumbinary planet detected from the ground, similar to the announced companions around close binaries HW Vir, NN Ser, UZ For, DP Leo, FS Aur or SZ Her, and planets of this type around Kepler-16, Kepler-34 and Kepler-35.

  2. Revised Safety Instruction 41 (IS41 REV.)

    CERN Multimedia

    SC Secretariat

    2005-01-01

    Please note that the Revised Safety Instruction No. 41 (IS41 REV.), entitled 'The use of plastic and other non-metallic materials at CERN with respect to fire safety and radiation resistance' is available on the web at the following url: https://edms.cern.ch/document/335806/LAST_RELEASED Paper copies can also be obtained from the SC Unit Secretariat, e-mail: sc.secretariat@cern.ch SC Secretariat

  3. The PhysTEC Teacher Education Program at FIU

    Science.gov (United States)

    Kramer, Laird

    2010-10-01

    The FIU PhysTEC Project is an integral component of the Physics Department's educational transformation that has led to more than a ten-fold increase in majors. The transformation seeks to increase the quality and quantity of physics majors and future physics teachers, including those from historically underrepresented groups. Elements of the efforts include transformed introductory physics courses, establishment of a physics research and learning community, engagement of stakeholders spanning high school through the university administration, and advocacy by a physics education research group. The PhysTEC Project supports future physics teachers through a Learning Assistant program coupled to newly revised secondary education programs. The Learning Assistant program is an experiential program that recruits new students into teaching careers while providing a mechanism for transforming courses - undergraduates experience the rewards and intellectual challenges of teaching through providing interactive engagement learning experiences for their peers in introductory physics courses. Students that continue in the program enroll in a multidisciplinary teacher preparation program and may receive significant financial support. FIU is a minority-serving urban public research institution in Miami, Florida serving over 39,000 students, of which 64% are Hispanic, 13% are Black, and 56% are women. Programmatic strategies and impacts of the program will be provided.

  4. Introducing RevPASH: The Free Webtool Application

    Directory of Open Access Journals (Sweden)

    Peter Szende

    2014-10-01

    Full Text Available RevPASH (Revenue Per Available Seat Hour is an important measure that helps restaurant operators understand how efficiently each seat in a restaurant generates revenue. The RevPASH app is an easy-to-use web-tool that provides an operator with a quick way to input a few relevant numbers and calculate RevPASH.The application has the ability to compare RevPASH over different times, days, weeks, and months.

  5. Publisher's Note: Search for ultrahigh energy neutrinos in highly inclined events at the Pierre Auger Observatory [Phys. Rev. D 84, 122005

    NARCIS (Netherlands)

    Abreu, P.; Aglietta, M.; Ahlers, M.; Ahn, E. J.; Albuquerque, I. F. M.; Allard, D.; Allekotte, I.; Allen, J.; Allison, P.; Almela, A.; Alvarez Castillo, J.; Alvarez-Muñiz, J.; Ambrosio, M.; Aminaei, A.; Anchordoqui, L.; Andringa, S.; Anticic, T.; Aramo, C.; Arganda, E.; Arqueros, F.; Asorey, H.; Assis, P.; Aublin, J.; Ave, M.; Avenier, M.; Avila, G.; Bäcker, T.; Badescu, A. M.; Balzer, M.; Barber, K. B.; Barbosa, A. F.; Bardenet, R.; Barroso, S. L. C.; Baughman, B.; Bäuml, J.; Beatty, J. J.; Becker, B. R.; Becker, K. H.; Bellétoile, A.; Bellido, J. A.; Benzvi, S.; Berat, C.; Bertou, X.; Biermann, P. L.; Billoir, P.; Blanco, F.; Blanco, M.; Bleve, C.; Blümer, H.; Bohácová, M.; Boncioli, D.; Bonifazi, C.; Bonino, R.; Borodai, N.; Brack, J.; Brancus, I.; Brogueira, P.; Brown, W. C.; Bruijn, R.; Buchholz, P.; Bueno, A.; Burton, R. E.; Caballero-Mora, K. S.; Caccianiga, B.; Caramete, L.; Caruso, R.; Castellina, A.; Catalano, O.; Cataldi, G.; Cazon, L.; Cester, R.; Chauvin, J.; Cheng, S. H.; Chiavassa, A.; Chinellato, J. A.; Chirinos Diaz, J.; Chudoba, J.; Clay, R. W.; Coluccia, M. R.; Conceição, R.; Contreras, F.; Cook, H.; Cooper, M. J.; Coppens, J.; Cordier, A.; Coutu, S.; Covault, C. E.; Creusot, A.; Criss, A.; Cronin, J.; Curutiu, A.; Dagoret-Campagne, S.; Dallier, R.; Dasso, S.; Daumiller, K.; Dawson, B. R.; de Almeida, R. M.; de Domenico, M.; de Donato, C.; de Jong, S. J.; de La Vega, G.; de Mello, W. J. M., Jr.; de Mello Neto, J. R. T.; de Mitri, I.; de Souza, V.; de Vries, K. D.; Del Peral, L.; Del Río, M.; Deligny, O.; Dembinski, H.; Dhital, N.; di Giulio, C.; Díaz Castro, M. L.; Diep, P. N.; Diogo, F.; Dobrigkeit, C.; Docters, W.; D'Olivo, J. C.; Dong, P. N.; Dorofeev, A.; Dos Anjos, J. C.; Dova, M. T.; D'Urso, D.; Dutan, I.; Ebr, J.; Engel, R.; Erdmann, M.; Escobar, C. O.; Espadanal, J.; Etchegoyen, A.; Facal San Luis, P.; Fajardo Tapia, I.; Falcke, H.; Farrar, G.; Fauth, A. C.; Fazzini, N.; Ferguson, A. P.; Fick, B.; Filevich, A.; Filipcic, A.; Fliescher, S.; Fracchiolla, C. E.; Fraenkel, E. D.; Fratu, O.; Fröhlich, U.; Fuchs, B.; Gaior, R.; Gamarra, R. F.; Gambetta, S.; García, B.; Garcia Roca, S. T.; Garcia-Gamez, D.; Garcia-Pinto, D.; Gascon, A.; Gemmeke, H.; Ghia, P. L.; Giaccari, U.; Giller, M.; Glass, H.; Gold, M. S.; Golup, G.; Gomez Albarracin, F.; Gómez Berisso, M.; Gómez Vitale, P. F.; Gonçalves, P.; Gonzalez, D.; Gonzalez, J. G.; Gookin, B.; Gorgi, A.; Gouffon, P.; Grashorn, E.; Grebe, S.; Griffith, N.; Grigat, M.; Grillo, A. F.; Guardincerri, Y.; Guarino, F.; Guedes, G. P.; Guzman, A.; Hansen, P.; Harari, D.; Harmsma, S.; Harrison, T. A.; Harton, J. L.; Haungs, A.; Hebbeker, T.; Heck, D.; Herve, A. E.; Hojvat, C.; Hollon, N.; Holmes, V. C.; Homola, P.; Hörandel, J. R.; Horneffer, A.; Horvath, P.; Hrabovský, M.; Huber, D.; Huege, T.; Insolia, A.; Ionita, F.; Italiano, A.; Jarne, C.; Jiraskova, S.; Josebachuili, M.; Kadija, K.; Kampert, K. H.; Karhan, P.; Kasper, P.; Kégl, B.; Keilhauer, B.; Keivani, A.; Kelley, J. L.; Kemp, E.; Kieckhafer, R. M.; Klages, H. O.; Kleifges, M.; Kleinfeller, J.; Knapp, J.; Koang, D.-H.; Kotera, K.; Krohm, N.; Krömer, O.; Kruppke-Hansen, D.; Kuehn, F.; Kuempel, D.; Kulbartz, J. K.; Kunka, N.; La Rosa, G.; Lachaud, C.; Lauer, R.; Lautridou, P.; Le Coz, S.; Leão, M. S. A. B.; Lebrun, D.; Lebrun, P.; Leigui de Oliveira, M. A.; Letessier-Selvon, A.; Lhenry-Yvon, I.; Link, K.; López, R.; Lopez Agüera, A.; Louedec, K.; Lozano Bahilo, J.; Lu, L.; Lucero, A.; Ludwig, M.; Lyberis, H.; Macolino, C.; Maldera, S.; Mandat, D.; Mantsch, P.; Mariazzi, A. G.; Marin, J.; Marin, V.; Maris, I. C.; Marquez Falcon, H. R.; Marsella, G.; Martello, D.; Martin, L.; Martinez, H.; Martínez Bravo, O.; Mathes, H. J.; Matthews, J.; Matthews, J. A. J.; Matthiae, G.; Maurel, D.; Maurizio, D.; Mazur, P. O.; Medina-Tanco, G.; Melissas, M.; Melo, D.; Menichetti, E.; Menshikov, A.; Mertsch, P.; Meurer, C.; Micanovic, S.; Micheletti, M. I.; Miramonti, L.; Molina-Bueno, L.; Mollerach, S.; Monasor, M.; Monnier Ragaigne, D.; Montanet, F.; Morales, B.; Morello, C.; Moreno, E.; Moreno, J. C.; Mostafá, M.; Moura, C. A.; Muller, M. A.; Müller, G.; Münchmeyer, M.; Mussa, R.; Navarra, G.; Navarro, J. L.; Navas, S.; Necesal, P.; Nellen, L.; Nelles, A.; Neuser, J.; Newton, D.; Nhung, P. T.; Niechciol, M.; Niemietz, L.; Nierstenhoefer, N.; Nitz, D.; Nosek, D.; Nožka, L.; Nyklicek, M.; Oehlschläger, J.; Olinto, A.; Ortiz, M.; Pacheco, N.; Pakk Selmi-Dei, D.; Palatka, M.; Pallotta, J.; Palmieri, N.; Parente, G.; Parizot, E.; Parra, A.; Pastor, S.; Paul, T.; Pech, M.; Pekala, J.; Pelayo, R.; Pepe, I. M.; Perrone, L.; Pesce, R.; Petermann, E.; Petrera, S.; Petrinca, P.; Petrolini, A.; Petrov, Y.; Pfendner, C.; Piegaia, R.; Pierog, T.; Pieroni, P.; Pimenta, M.

    2012-01-01

    The Surface Detector of the Pierre Auger Observatory is sensitive to neutrinos of all flavours above 0.1 EeV. These interact through charged and neutral currents in the atmosphere giving rise to extensive air showers. When interacting deeply in the atmosphere at nearly horizontal incidence, neutrino

  6. Comment on Y. Couder and E. Fort: "Single-Particle Diffraction and Interference at a Macroscopic Scale", Phys. Rev. Lett. (2006)

    CERN Document Server

    Andersen, Anders; Reichelt, Christian; Ahl, Sonja Rosenlund; Lautrup, Benny; Ellegaard, Clive; Levinsen, Mogens T; Bohr, Tomas

    2014-01-01

    In a paper from 2006, Couder and Fort [1] describe a version of the famous double slit experiment performed with drops bouncing on a vibrated fluid surface, where interference in the particle statistics is found even though it is possible to determine unambiguously which slit the "walking" drop passes. It is one of the first papers in an impressive series, showing that such walking drops closely resemble de Broglie waves and can reproduce typical quantum phenomena like tunneling and quantized states [2-13]. The double slit experiment is, however, a more stringent test of quantum mechanics, because it relies upon superposition and phase coherence. In the present comment we first point out that the experimental data presented in [1] are not convincing, and secondly we argue that it is not possible in general to capture quantum mechanical results in a system, where the trajectory of the particle is well-defined.

  7. Comment on "Observation of a push force on the end face of a nanometer silica filament exerted by outgoing light," Phys. Rev. Lett. 101, 243601 (2008)

    CERN Document Server

    Mansuripur, Masud

    2012-01-01

    In a recent paper, W. She, J. Yu and R. Feng reported the slight deformations observed upon transmission of a light pulse through a fairly short length of a silica glass nano-fiber. Relating the shape and magnitude of these deformations to the momentum of the light pulse both inside and outside the fiber, these authors concluded that, within the fiber, the photons carry the Abraham momentum. In my view, the authors' claim that they have resolved the Abraham-Minkowski controversy surrounding the momentum of photons inside dielectric media is premature. A correct interpretation of the experiments of She et al requires precise calculations that would properly account not only for the electromagnetic momentum (both inside and outside the fiber) but also for the Lorentz force exerted on the fiber by the light pulse in its entire path through this nano-waveguide.

  8. Introduction to "contextual values" and a simpler counterexample to a claim of Dressel, Agarwal, and Jordan [Phys. Rev. Lett. 104 240401 (2010)

    CERN Document Server

    Parrott, Stephen

    2011-01-01

    The abstract of the paper mentioned in the title, called DAJ below, states: "We introduce contextual values as a generalization of the eigenvalues of an observable that takes into account both the system observable and a general measurement procedure. This technique leads to a natural definition of a general conditioned average that converges uniquely to the quantum weak value in the minimal disturbance limit." A counterexample to the claim of the last sentence was presented in arXiv:1102.4407v6, a 32-page paper discussing various topics related to DAJ. The counterexample relied on a fairly complicated solution of a system of linear equations with algebraic coefficients, and so was not entirely intuitive. The second half of the present note gives a simplified counterexample, all of whose steps can be verified mentally. The first half summarizes the main ideas of DAJ.

  9. Publisher's Note: Search for ultrahigh energy neutrinos in highly inclined events at the Pierre Auger Observatory [Phys. Rev. D 84, 122005 (2011)

    Science.gov (United States)

    Abreu, P.; Aglietta, M.; Ahlers, M.; Ahn, E. J.; Albuquerque, I. F. M.; Allard, D.; Allekotte, I.; Allen, J.; Allison, P.; Almela, A.; Alvarez Castillo, J.; Alvarez-Muñiz, J.; Ambrosio, M.; Aminaei, A.; Anchordoqui, L.; Andringa, S.; Anticic, T.; Aramo, C.; Arganda, E.; Arqueros, F.; Asorey, H.; Assis, P.; Aublin, J.; Ave, M.; Avenier, M.; Avila, G.; Bäcker, T.; Badescu, A. M.; Balzer, M.; Barber, K. B.; Barbosa, A. F.; Bardenet, R.; Barroso, S. L. C.; Baughman, B.; Bäuml, J.; Beatty, J. J.; Becker, B. R.; Becker, K. H.; Bellétoile, A.; Bellido, J. A.; Benzvi, S.; Berat, C.; Bertou, X.; Biermann, P. L.; Billoir, P.; Blanco, F.; Blanco, M.; Bleve, C.; Blümer, H.; Bohácová, M.; Boncioli, D.; Bonifazi, C.; Bonino, R.; Borodai, N.; Brack, J.; Brancus, I.; Brogueira, P.; Brown, W. C.; Bruijn, R.; Buchholz, P.; Bueno, A.; Burton, R. E.; Caballero-Mora, K. S.; Caccianiga, B.; Caramete, L.; Caruso, R.; Castellina, A.; Catalano, O.; Cataldi, G.; Cazon, L.; Cester, R.; Chauvin, J.; Cheng, S. H.; Chiavassa, A.; Chinellato, J. A.; Chirinos Diaz, J.; Chudoba, J.; Clay, R. W.; Coluccia, M. R.; Conceição, R.; Contreras, F.; Cook, H.; Cooper, M. J.; Coppens, J.; Cordier, A.; Coutu, S.; Covault, C. E.; Creusot, A.; Criss, A.; Cronin, J.; Curutiu, A.; Dagoret-Campagne, S.; Dallier, R.; Dasso, S.; Daumiller, K.; Dawson, B. R.; de Almeida, R. M.; de Domenico, M.; de Donato, C.; de Jong, S. J.; de La Vega, G.; de Mello, W. J. M., Jr.; de Mello Neto, J. R. T.; de Mitri, I.; de Souza, V.; de Vries, K. D.; Del Peral, L.; Del Río, M.; Deligny, O.; Dembinski, H.; Dhital, N.; di Giulio, C.; Díaz Castro, M. L.; Diep, P. N.; Diogo, F.; Dobrigkeit, C.; Docters, W.; D'Olivo, J. C.; Dong, P. N.; Dorofeev, A.; Dos Anjos, J. C.; Dova, M. T.; D'Urso, D.; Dutan, I.; Ebr, J.; Engel, R.; Erdmann, M.; Escobar, C. O.; Espadanal, J.; Etchegoyen, A.; Facal San Luis, P.; Fajardo Tapia, I.; Falcke, H.; Farrar, G.; Fauth, A. C.; Fazzini, N.; Ferguson, A. P.; Fick, B.; Filevich, A.; Filipcic, A.; Fliescher, S.; Fracchiolla, C. E.; Fraenkel, E. D.; Fratu, O.; Fröhlich, U.; Fuchs, B.; Gaior, R.; Gamarra, R. F.; Gambetta, S.; García, B.; Garcia Roca, S. T.; Garcia-Gamez, D.; Garcia-Pinto, D.; Gascon, A.; Gemmeke, H.; Ghia, P. L.; Giaccari, U.; Giller, M.; Glass, H.; Gold, M. S.; Golup, G.; Gomez Albarracin, F.; Gómez Berisso, M.; Gómez Vitale, P. F.; Gonçalves, P.; Gonzalez, D.; Gonzalez, J. G.; Gookin, B.; Gorgi, A.; Gouffon, P.; Grashorn, E.; Grebe, S.; Griffith, N.; Grigat, M.; Grillo, A. F.; Guardincerri, Y.; Guarino, F.; Guedes, G. P.; Guzman, A.; Hansen, P.; Harari, D.; Harmsma, S.; Harrison, T. A.; Harton, J. L.; Haungs, A.; Hebbeker, T.; Heck, D.; Herve, A. E.; Hojvat, C.; Hollon, N.; Holmes, V. C.; Homola, P.; Hörandel, J. R.; Horneffer, A.; Horvath, P.; Hrabovský, M.; Huber, D.; Huege, T.; Insolia, A.; Ionita, F.; Italiano, A.; Jarne, C.; Jiraskova, S.; Josebachuili, M.; Kadija, K.; Kampert, K. H.; Karhan, P.; Kasper, P.; Kégl, B.; Keilhauer, B.; Keivani, A.; Kelley, J. L.; Kemp, E.; Kieckhafer, R. M.; Klages, H. O.; Kleifges, M.; Kleinfeller, J.; Knapp, J.; Koang, D.-H.; Kotera, K.; Krohm, N.; Krömer, O.; Kruppke-Hansen, D.; Kuehn, F.; Kuempel, D.; Kulbartz, J. K.; Kunka, N.; La Rosa, G.; Lachaud, C.; Lauer, R.; Lautridou, P.; Le Coz, S.; Leão, M. S. A. B.; Lebrun, D.; Lebrun, P.; Leigui de Oliveira, M. A.; Letessier-Selvon, A.; Lhenry-Yvon, I.; Link, K.; López, R.; Lopez Agüera, A.; Louedec, K.; Lozano Bahilo, J.; Lu, L.; Lucero, A.; Ludwig, M.; Lyberis, H.; Macolino, C.; Maldera, S.; Mandat, D.; Mantsch, P.; Mariazzi, A. G.; Marin, J.; Marin, V.; Maris, I. C.; Marquez Falcon, H. R.; Marsella, G.; Martello, D.; Martin, L.; Martinez, H.; Martínez Bravo, O.; Mathes, H. J.; Matthews, J.; Matthews, J. A. J.; Matthiae, G.; Maurel, D.; Maurizio, D.; Mazur, P. O.; Medina-Tanco, G.; Melissas, M.; Melo, D.; Menichetti, E.; Menshikov, A.; Mertsch, P.; Meurer, C.; Micanovic, S.; Micheletti, M. I.; Miramonti, L.; Molina-Bueno, L.; Mollerach, S.; Monasor, M.; Monnier Ragaigne, D.; Montanet, F.; Morales, B.; Morello, C.; Moreno, E.; Moreno, J. C.; Mostafá, M.; Moura, C. A.; Muller, M. A.; Müller, G.; Münchmeyer, M.; Mussa, R.; Navarra, G.; Navarro, J. L.; Navas, S.; Necesal, P.; Nellen, L.; Nelles, A.; Neuser, J.; Newton, D.; Nhung, P. T.; Niechciol, M.; Niemietz, L.; Nierstenhoefer, N.; Nitz, D.; Nosek, D.; Nožka, L.; Nyklicek, M.; Oehlschläger, J.; Olinto, A.; Ortiz, M.; Pacheco, N.; Pakk Selmi-Dei, D.; Palatka, M.; Pallotta, J.; Palmieri, N.; Parente, G.; Parizot, E.; Parra, A.; Pastor, S.; Paul, T.; Pech, M.; Pekala, J.; Pelayo, R.; Pepe, I. M.; Perrone, L.; Pesce, R.; Petermann, E.; Petrera, S.; Petrinca, P.; Petrolini, A.; Petrov, Y.; Pfendner, C.; Piegaia, R.; Pierog, T.; Pieroni, P.; Pimenta, M.; Pirronello, V.; Platino, M.; Ponce, V. H.; Pontz, M.; Porcelli, A.; Privitera, P.; Prouza, M.; Quel, E. J.; Querchfeld, S.; Rautenberg, J.; Ravel, O.; Ravignani, D.; Revenu, B.; Ridky, J.; Riggi, S.; Risse, M.; Ristori, P.; Rivera, H.; Rizi, V.; Roberts, J.; Rodrigues de Carvalho, W.; Rodriguez, G.; Rodriguez Martino, J.; Rodriguez Rojo, J.; Rodriguez-Cabo, I.; Rodríguez-Frías, M. D.; Ros, G.; Rosado, J.; Rossler, T.; Roth, M.; Rouillé-D'Orfeuil, B.; Roulet, E.; Rovero, A. C.; Rühle, C.; Saftoiu, A.; Salamida, F.; Salazar, H.; Salesa Greus, F.; Salina, G.; Sánchez, F.; Santo, C. E.; Santos, E.; Santos, E. M.; Sarazin, F.; Sarkar, B.; Sarkar, S.; Sato, R.; Scharf, N.; Scherini, V.; Schieler, H.; Schiffer, P.; Schmidt, A.; Scholten, O.; Schoorlemmer, H.; Schovancova, J.; Schovánek, P.; Schröder, F.; Schulte, S.; Schuster, D.; Sciutto, S. J.; Scuderi, M.; Segreto, A.; Settimo, M.; Shadkam, A.; Shellard, R. C.; Sidelnik, I.; Sigl, G.; Silva Lopez, H. H.; Sima, O.; Smialkowski, A.; Šmída, R.; Snow, G. R.; Sommers, P.; Sorokin, J.; Spinka, H.; Squartini, R.; Srivastava, Y. N.; Stanic, S.; Stapleton, J.; Stasielak, J.; Stephan, M.; Stutz, A.; Suarez, F.; Suomijärvi, T.; Supanitsky, A. D.; Šuša, T.; Sutherland, M. S.; Swain, J.; Szadkowski, Z.; Szuba, M.; Tapia, A.; Tartare, M.; Tascau, O.; Tavera Ruiz, C. G.; Tcaciuc, R.; Tegolo, D.; Thao, N. T.; Thomas, D.; Tiffenberg, J.; Timmermans, C.; Tkaczyk, W.; Todero Peixoto, C. J.; Toma, G.; Tomé, B.; Tonachini, A.; Travnicek, P.; Tridapalli, D. B.; Tristram, G.; Trovato, E.; Tueros, M.; Ulrich, R.; Unger, M.; Urban, M.; Valdés Galicia, J. F.; Valiño, I.; Valore, L.; van den Berg, A. M.; Varela, E.; Vargas Cárdenas, B.; Vázquez, J. R.; Vázquez, R. A.; Veberic, D.; Verzi, V.; Vicha, J.; Videla, M.; Villaseñor, L.; Wahlberg, H.; Wahrlich, P.; Wainberg, O.; Walz, D.; Watson, A. A.; Weber, M.; Weidenhaupt, K.; Weindl, A.; Werner, F.; Westerhoff, S.; Whelan, B. J.; Widom, A.; Wieczorek, G.; Wiencke, L.; Wilczynska, B.; Wilczynski, H.; Will, M.; Williams, C.; Winchen, T.; Wommer, M.; Wundheiler, B.; Yamamoto, T.; Yapici, T.; Younk, P.; Yuan, G.; Yushkov, A.; Zamorano, B.; Zas, E.; Zavrtanik, D.; Zavrtanik, M.; Zaw, I.; Zepeda, A.; Zhu, Y.; Zimbres Silva, M.; Ziolkowski, M.

    2012-01-01

    The Surface Detector of the Pierre Auger Observatory is sensitive to neutrinos of all flavours above 0.1 EeV. These interact through charged and neutral currents in the atmosphere giving rise to extensive air showers. When interacting deeply in the atmosphere at nearly horizontal incidence, neutrinos can be distinguished from regular hadronic cosmic rays by the broad time structure of their shower signals in the water-Cherenkov detectors. In this paper we present for the first time an analysis based on down-going neutrinos. We describe the search procedure, the possible sources of background, the method to compute the exposure and the associated systematic uncertainties. No candidate neutrinos have been found in data collected from 1 January 2004 to 31 May 2010. Assuming an E^-2 differential energy spectrum the limit on the single flavour neutrino is (E^2 * dN/dE) < 1.74x10^-7 GeV cm^-2 s^-1 sr^-1 at 90% C.L. in the energy range 1x10^17 eV < E < 1x10^20 eV.

  10. Comments on ``The Euclidean gravitational action as black hole entropy, singularities, and space-time voids'' [J. Math. Phys. 49, 042501 (2008)

    Science.gov (United States)

    Mitra, Abhas

    2009-04-01

    We point out that the space-time void inferred by Castro [J. Math. Phys. 49, 042501 (2008)] results from his choice of a discontinuous radial gauge. Further since the integration constant α0=2M0 (G =c=1) occurring in the vacuum Hilbert/Schwarzschild solution of a neutral "point mass" is zero [Arnowitt et al., in Gravitation: An Introduction to Current Research, edited by L. Witten (Wiley, New York, 1962), Chap. 7, p. 227; also Phys. Rev. Lett. 4, 375 (1960). A. Mitra, Adv. Space Res. 38, 2917 (2006); Proceedings of the XIth Marcel-Grossmann Conference on General Relativity (World Scientific, Singapore, 2008), Vol. 3, p. 1968], Castro's gauge reduces to the well behaved and physical Hilbert gauge. Physically this means that true Hilbert/Schwarzschild black holes have unique gravitational mass M =0. Accordingly, the unphysical space-time void inferred by Castro is actually nonexistent.

  11. Hu Shi's Study of Chinese Medieval intellectual History

    Institute of Scientific and Technical Information of China (English)

    Lou Yulie

    2006-01-01

    Hu Shi frequently gave lectures on the history of Chinese philosophy,especially the history of ancient Chinese philosophy,from the year 1919 to 1937.A large number of papers and dissertations published during this period are related to his research on this topic.In his opinion,there are three characteristics of the history of ancient Chinese philosophy:" religionalization of thought," "Indianization of philosophy," and "conflict between Chinese thought and Indian thought." In this paper,I explore Hu Shi's deep insight into the religionalization of Confucianism in Han dynasty and into the thought of Taoism in the medieval times.

  12. Treatment of β-Thalassemia With Hydroxyurea (HU)——Effects of HU on Globin Gene Expression

    Institute of Scientific and Technical Information of China (English)

    黄淑帧; 任兆瑞; 陈美珏; 许洪平; 曾溢滔; G.P.Rodgers; 曾凡一; A.N.Schechter

    1994-01-01

    A newly developed method of RT-PCR/competitive PCR for measuring the relative and ab-solute content of globin mRNAs as well as micro-globin chain biosynthetic assay have been used to study thealterations of globin gene expressions in the patients with β-thalassemia pre-and post-hydroxyurea(HU)treatment.It was found for the first time that HU had the effect of enhancing β-globin gene expression insome patients.Two cases with β-thalassemia who were subjected to HU treatment for over two years showeda marked increase in β-globin mRNA level and β-globin chain synthesis,resulting in more effective erythro-poiesis and the alleviation of clinical symptoms.

  13. Suppression of atherosclerosis by synthetic REV-ERB agonist

    Energy Technology Data Exchange (ETDEWEB)

    Sitaula, Sadichha [Department of Molecular Therapeutics, The Scripps Research Institute, Jupiter, FL 33458 (United States); Billon, Cyrielle [Department of Pharmacological & Physiological Science, Saint Louis University School of Medicine, St. Louis, MO 63104 (United States); Kamenecka, Theodore M.; Solt, Laura A. [Department of Molecular Therapeutics, The Scripps Research Institute, Jupiter, FL 33458 (United States); Burris, Thomas P., E-mail: burristp@slu.edu [Department of Pharmacological & Physiological Science, Saint Louis University School of Medicine, St. Louis, MO 63104 (United States)

    2015-05-08

    The nuclear receptors for heme, REV-ERBα and REV-ERBβ, play important roles in the regulation of metabolism and inflammation. Recently it was demonstrated that reduced REV-ERBα expression in hematopoetic cells in LDL receptor null mice led to increased atherosclerosis. We sought to determine if synthetic REV-ERB agonists that we have developed might have the ability to suppress atherosclerosis in this model. A previously characterized synthetic REV-ERB agonist, SR9009, was used to determine if activation of REV-ERB activity would affect atherosclerosis in LDL receptor deficient mice. Atherosclerotic plaque size was significantly reduced (p < 0.05) in mice administered SR9009 (100 mg/kg) for seven weeks compared to control mice (n = 10 per group). SR9009 treatment of bone marrow-derived mouse macrophages (BMDM) reduced the polarization of BMDMs to proinflammatory M1 macrophage while increasing the polarization of BMDMs to anti-inflammatory M2 macrophages. Our results suggest that pharmacological targeting of REV-ERBs may be a viable therapeutic option for treatment of atherosclerosis. - Highlights: • Synthetic REV-ERB agonist treatment reduced atherosclerosis in a mouse model. • Pharmacological activation of REV-ERB decreased M1 macrophage polarization. • Pharmacological activation of REV-ERB increased M2 macrophage polarization.

  14. HU-444, a Novel, Potent Anti-Inflammatory, Nonpsychotropic Cannabinoid.

    Science.gov (United States)

    Haj, Christeene G; Sumariwalla, Percy F; Hanuš, Lumír; Kogan, Natalya M; Yektin, Zhana; Mechoulam, Raphael; Feldmann, Mark; Gallily, Ruth

    2015-10-01

    Cannabidiol (CBD) is a component of cannabis, which does not cause the typical marijuana-type effects, but has a high potential for use in several therapeutic areas. In contrast to Δ(9)-tetrahydrocannabinol (Δ(9)-THC), it binds very weakly to the CB1 and CB2 cannabinoid receptors. It has potent activity in both in vitro and in vivo anti-inflammatory assays. Thus, it lowers the formation of tumor necrosis factor (TNF)-α, a proinflammatory cytokine, and was found to be an oral antiarthritic therapeutic in murine collagen-induced arthritis in vivo. However, in acidic media, it can cyclize to the psychoactive Δ(9)-THC. We report the synthesis of a novel CBD derivative, HU-444, which cannot be converted by acid cyclization into a Δ(9)-THC-like compound. In vitro HU-444 had anti-inflammatory activity (decrease of reactive oxygen intermediates and inhibition of TNF-α production by macrophages); in vivo it led to suppression of production of TNF-α and amelioration of liver damage as well as lowering of mouse collagen-induced arthritis. HU-444 did not cause Δ(9)-THC-like effects in mice. We believe that HU-444 represents a potential novel drug for rheumatoid arthritis and other inflammatory diseases.

  15. HuR in pheochromocytomas and paragangliomas - overexpression in verified malignant tumors.

    Science.gov (United States)

    Leijon, Helena; Salmenkivi, Kaisa; Heiskanen, Ilkka; Hagström, Jaana; Louhimo, Johanna; Heikkilä, Päivi; Ristimäki, Ari; Paavonen, Timo; Metso, Saara; Mäenpää, Hanna; Haglund, Caj; Arola, Johanna

    2016-09-01

    Pheochromocytomas and paragangliomas are rare, neural crest-originating, neuroendocrine tumors. HuR is an mRNA-binding protein of the ELAV/Hu-protein family, which participates in posttranscriptional regulation of many cancer-associated genes. HuR expression has been connected with aggressive behavior of several malignancies. Cyclooxygenase-2 (COX-2) is also expressed in several malignant tumors, and its expression is regulated by HuR. Tissue microarray of 153 primary pheochromocytomas and paragangliomas was investigated for the expression of HuR and COX-2 proteins by immunohistochemistry using two different HuR antibodies (HuR19F12 and HuR3A). In these tumors, the expression of both intranuclear and cytoplasmic HuR was detectable. Increased cytoplasmic HuR expression was significantly associated with metastatic tumors. Increased COX-2 and MIB-1 expression also was associated with metastatic potential, and moreover, HuR and COX-2 expression correlated with each other. Our data suggest that increased expression of HuR protein is associated with metastatic potential of paragangliomas and pheochromocytomas, and COX-2 seems to be a target of HuR.

  16. Structure-based analysis of HU-DNA binding.

    Science.gov (United States)

    Swinger, Kerren K; Rice, Phoebe A

    2007-01-26

    HU and IHF are prokaryotic proteins that induce very large bends in DNA. They are present in high concentrations in the bacterial nucleoid and aid in chromosomal compaction. They also function as regulatory cofactors in many processes, such as site-specific recombination and the initiation of replication and transcription. HU and IHF have become paradigms for understanding DNA bending and indirect readout of sequence. While IHF shows significant sequence specificity, HU binds preferentially to certain damaged or distorted DNAs. However, none of the structurally diverse HU substrates previously studied in vitro is identical with the distorted substrates in the recently published Anabaena HU(AHU)-DNA cocrystal structures. Here, we report binding affinities for AHU and the DNA in the cocrystal structures. The binding free energies for formation of these AHU-DNA complexes range from approximately 10-14.5 kcal/mol, representing K(d) values in the nanomolar to low picomolar range, and a maximum stabilization of at least approximately 6.3 kcal/mol relative to complexes with undistorted, non-specific DNA. We investigated IHF binding and found that appropriate structural distortions can greatly enhance its affinity. On the basis of the coupling of structural and relevant binding data, we estimate the amount of conformational strain in an IHF-mediated DNA kink that is relieved by a nick (at least 0.76 kcal/mol) and pinpoint the location of the strain. We show that AHU has a sequence preference for an A+T-rich region in the center of its DNA-binding site, correlating with an unusually narrow minor groove. This is similar to sequence preferences shown by the eukaryotic nucleosome.

  17. Structure-based Analysis to Hu-DNA Binding

    Energy Technology Data Exchange (ETDEWEB)

    Swinger,K.; Rice, P.

    2007-01-01

    HU and IHF are prokaryotic proteins that induce very large bends in DNA. They are present in high concentrations in the bacterial nucleoid and aid in chromosomal compaction. They also function as regulatory cofactors in many processes, such as site-specific recombination and the initiation of replication and transcription. HU and IHF have become paradigms for understanding DNA bending and indirect readout of sequence. While IHF shows significant sequence specificity, HU binds preferentially to certain damaged or distorted DNAs. However, none of the structurally diverse HU substrates previously studied in vitro is identical with the distorted substrates in the recently published Anabaena HU(AHU)-DNA cocrystal structures. Here, we report binding affinities for AHU and the DNA in the cocrystal structures. The binding free energies for formation of these AHU-DNA complexes range from 10-14.5 kcal/mol, representing K{sub d} values in the nanomolar to low picomolar range, and a maximum stabilization of at least 6.3 kcal/mol relative to complexes with undistorted, non-specific DNA. We investigated IHF binding and found that appropriate structural distortions can greatly enhance its affinity. On the basis of the coupling of structural and relevant binding data, we estimate the amount of conformational strain in an IHF-mediated DNA kink that is relieved by a nick (at least 0.76 kcal/mol) and pinpoint the location of the strain. We show that AHU has a sequence preference for an A+T-rich region in the center of its DNA-binding site, correlating with an unusually narrow minor groove. This is similar to sequence preferences shown by the eukaryotic nucleosome.

  18. HU-GFP and DAPI co-localize on the Escherichia coli nucleoid.

    Science.gov (United States)

    Wery, M; Woldringh, C L; Rouviere-Yaniv, J

    2001-02-01

    The heterodimeric HU protein, one of the most abundant DNA binding proteins, plays a pleiotropic role in bacteria. Among others, HU was shown to contribute to the maintenance of DNA superhelical density in Escherichia coli. By its properties HU shares some traits with histones and HMG proteins. More recently, its specific binding to DNA recombination and repair intermediates suggests that HU should be considered as a DNA damage sensor. For all these reasons, it will be of interest to follow the localization of HU within the living bacterial cells. To this end, we constructed HU-GFP fusion proteins and compared by microscopy the GFP green fluorescence with images of the nucleoid after DAPI staining. We show that DAPI and HU-GFP colocalize on the E. coli nucleoid. HU, therefore, can be considered as a natural tracer of DNA in the living bacterial cell.

  19. Targeting Mycobacterium tuberculosis nucleoid-associated protein HU with structure-based inhibitors

    Science.gov (United States)

    Bhowmick, Tuhin; Ghosh, Soumitra; Dixit, Karuna; Ganesan, Varsha; Ramagopal, Udupi A.; Dey, Debayan; Sarma, Siddhartha P.; Ramakumar, Suryanarayanarao; Nagaraja, Valakunja

    2014-06-01

    The nucleoid-associated protein HU plays an important role in maintenance of chromosomal architecture and in global regulation of DNA transactions in bacteria. Although HU is essential for growth in Mycobacterium tuberculosis (Mtb), there have been no reported attempts to perturb HU function with small molecules. Here we report the crystal structure of the N-terminal domain of HU from Mtb. We identify a core region within the HU-DNA interface that can be targeted using stilbene derivatives. These small molecules specifically inhibit HU-DNA binding, disrupt nucleoid architecture and reduce Mtb growth. The stilbene inhibitors induce gene expression changes in Mtb that resemble those induced by HU deficiency. Our results indicate that HU is a potential target for the development of therapies against tuberculosis.

  20. Administrative Circular No. 14 (Rev. 2)

    CERN Multimedia

    HR Department

    2007-01-01

    The HR Department wishes to draw the attention of members of the personnel to a number of amendments to Administrative Circular No. 14 (Rev. 2) entitled "Protection of members of the personnel against the financial consequences of illness, accident and disability" which came into force on 1st July 2006 (cf. Weekly Bulletin of 14 and 21 August 2006). Occupational Accident Declaration Form (HS50) https://cern.ch/service-procedures/AdminMan/Forms/HS50E.doc •\tIt must be completed within 10 working days of the date on which the accident occurred (§ 29.2.1), unless the person concerned is materially unable to meet this deadline. • The completed formula must be accompanied by a medical certificate giving details of any bodily injuries resulting from the accident (Annex 1, § 5). The medical certificate must be obtained from the doctor who has been consulted for that purpose. Benefits resulting from illnesses and accidents Medical treatment will cease to be reimbursed under ...

  1. Genesis Research与Morphosys扩展HuCAL抗体协议

    Institute of Scientific and Technical Information of China (English)

    黄晓燕(摘)

    2008-01-01

    Genesis Research与Morphosys扩展协议后,靶向FGFR5蛋白的抗体将继续作为其研究的焦点。Genesis将利用Morphosys分公司Serotec开发的人组织抗体库(HuCAL)继续开发成纤维细胞生长因子受体5(FGFR5)靶点。

  2. Preface: phys. stat. sol. (a) 202/12

    Science.gov (United States)

    Neumann, Wolfgang; Stutzmann, Martin; Hildebrandt, Stefan

    2005-09-01

    The present special issue contains a collection of Original Papers dedicated to Professor Johannes Heydenreich on the occasion of his 75th birthday.Johannes Heydenreich, born on 20 June 1930 in Plauen/Vogtland near Dresden, studied physics at the Pädagogische Hochschule Potsdam, where he obtained his first academic degree Dipl. Phys. in 1958. He received his doctoral degree at the Martin Luther University in Halle in 1961 and the Habilitation degree in 1969. Already during his studies in Potsdam, he showed an interest in electron microscopy due to the influence of his teacher and supervisor Prof. Picht, one of the pioneers in electron optics. His interests were strengthened when Johannes Heydenreich did the experimental work for his Diploma degree at the Institute for Experimental Physics of the University of Halle, where he met Prof. Heinz Bethge for the first time. This was the beginning of a fruitful and longstanding collaboration. In 1962 Johannes Heydenreich joined the team of the later Institute for Solid State Physics and Electron Microscopy of the Academy of Sciences of the GDR, in Halle, for which the basis was laid by Prof. Bethge in 1960.Heydenreich has been working as Assistant Director for many years and played a decisive role in introducing and organising the various techniques of electron microscopy in the institute.The research activities of Prof. Heydenreich covered a broad spectrum over the years. At the beginning of his career he made significant contributions in the field of electron mirror microscopy. After that, his main interests were focused on transmission electron microscopy, ranging from diffraction contrast analysis of crystal defects to high-resolution electron microscopy and image processing. His favourite field was studies of defect-induced phenomena in advanced materials. The so-called Bethge-Heydenreich, the book Electron Microscopy in Solid State Physics, published at first in a German edition in 1982 and later in a revised

  3. Rev-erbα and Rev-erbβ coordinately protect the circadian clock and normal metabolic function

    DEFF Research Database (Denmark)

    Bugge, Anne Skovsø; Feng, Dan; Everett, Logan J;

    2012-01-01

    of binding sites across the genome, enriched near metabolic genes. Depletion of both Rev-erbs in liver synergistically derepresses several metabolic genes as well as genes that control the positive limb of the molecular clock. Moreover, deficiency of both Rev-erbs causes marked hepatic steatosis, in contrast...

  4. Wave power plant at Horns Rev. Screening[Denmark]; Boelgekraftanlaeg ved Horns Rev. Screening

    Energy Technology Data Exchange (ETDEWEB)

    Soerensen, Hans C.; Nielsen, Kim; Steenstrup, P.R.; Friis-Madsen, E.; Wigant, L.

    2005-12-15

    The objective for the analysis has been to establish data for the sea at Horns Rev wind farm in the North Sea in order to assess the opportunity for using the site as test site for demonstration of wave energy devices exemplified by three different devices under development in Denmark. For comparison alternative sites like Hanstholm, Samsoe and Nissum Bredning are also assessed as well as the test centre EMEC at the Orkney Islands and the proposed test site Wave Hub at the north coast of Cornwall. The analysis shows that it is possible without major technical problems to connect 2-4 MW power generated by 3 different wave energy devices (AquaBuOY, Wave Star Energy and Wave Dragon) to the wind farm at Horns Rev (www.hornsrev.dk). The expenses for connection and regulation within the wind farm is about 200,000 DKK (30,00 EURO). On top of this comes the cost for individual sub sea cable connection to the wave devices, pull in of the sub sea cable through the existing J-tube in turbine T04 and the necessary regulation/control system in the individual wave devices to avoid damaging the power system in case of too high production. The analysis of the co-production of wind and wave power is dealt with in a separate report which shows that over a time period of half to one hour the time variation for wind generated electricity is 3 times as large as for wave energy generated power based on the actual measurement at Horns Rev. Further on the analysis shows that the wave generated power is more predictable than wind energy generated power as the power from the waves first is present about 2 hours after the wind is acting and last for 3 to 6 hours after the wind dies out; 6 to 12 hours with wind from west. The time is off course strongly depending of the direction of the wind i.e. the fetch. As this special report has a more general scope than the analysis as such it is reported in English (Annex Report II). The analysis shows that it is up to the individual device developer

  5. DNA nuclease activity of Rev-coupled transition metal chelates.

    Science.gov (United States)

    Joyner, Jeff C; Keuper, Kevin D; Cowan, J A

    2012-06-07

    Artificial nucleases containing Rev-coupled metal chelates based on combinations of the transition metals Fe(2+), Co(2+), Ni(2+), and Cu(2+) and the chelators DOTA, DTPA, EDTA, NTA, tripeptide GGH, and tetrapeptide KGHK have been tested for DNA nuclease activity. Originally designed to target reactive transition metal chelates (M-chelates) to the HIV-1 Rev response element mRNA, attachment to the arginine-rich Rev peptide also increases DNA-binding affinity for the attached M-chelates. Apparent K(D) values ranging from 1.7 to 3.6 µM base pairs for binding of supercoiled pUC19 plasmid DNA by Ni-chelate-Rev complexes were observed, as a result of electrostatic attraction between the positively-charged Rev peptide and negatively-charged DNA. Attachment of M-chelates to the Rev peptide resulted in enhancements of DNA nuclease activity ranging from 1-fold (no enhancement) to at least 13-fold (for Cu-DTPA-Rev), for the rate of DNA nicking, with second order rate constants for conversion of DNA(supercoiled) to DNA(nicked) up to 6 × 10(6) M(-1) min(-1), and for conversion of DNA(nicked) to DNA(linear) up to 1 × 10(5) M(-1) min(-1). Freifelder-Trumbo analysis and the ratios of linearization and nicking rate constants (k(lin)/k(nick)) revealed concerted mechanisms for nicking and subsequent linearization of plasmid DNA for all of the Rev-coupled M-chelates, consistent with higher DNA residency times for the Rev-coupled M-chelates. Observed rates for Rev-coupled M-chelates were less skewed by differing DNA-binding affinities than for M-chelates lacking Rev, as a result of the narrow range of DNA-binding affinities observed, and therefore relationships between DNA nuclease activity and other catalyst properties, such as coordination unsaturation, the ability to consume ascorbic acid and generate diffusible radicals, and the identity of the metal center, are now clearly illustrated in light of the similar DNA-binding affinities of all M-chelate-Rev complexes. This work

  6. HuCOP1 contributes to the regulation of DNA repair in keratinocytes.

    Science.gov (United States)

    Fazekas, B; Carty, M P; Németh, I; Kemény, L; Széll, M; Ádám, É

    2017-03-01

    We have previously demonstrated that the E3 ligase Human Constitutive Photomorphogenic Protein (huCOP1) is expressed in human keratinocytes and negatively regulates p53. The MutS homolog 2 (MSH2) protein plays a central role in DNA MMR mechanism and is implicated in the cellular response to anticancer agents, such as cisplatin. Our aim was to clarify whether huCOP1 plays a role in DNA MMR by affecting MSH2 protein level in human keratinocytes. To define the role of huCOP1 in DNA mismatch repair, we determined whether huCOP1 affects MSH2 abundance. MSH2 protein level was detected by immunocytochemical staining using a keratinocyte cell line in which the expression level of huCOP1 was stably decreased (siCOP1). To investigate whether huCOP1 silencing influences cisplatin-induced cell death, control and siCOP1 keratinocyte cells were treated with increasing concentrations of cisplatin and cell viability was recorded after 48 and 96 h. Stable silencing of huCOP1 in human keratinocytes resulted in a reduced level of MSH2 protein. huCOP1 silencing also sensitized keratinocytes to the interstrand crosslinking inducer cisplatin. Our results indicate that decreased huCOP1 correlates with lower MSH2 levels. These protein level changes lead to increased sensitivity toward cisplatin treatment, implicating that huCOP1 plays a positive role in maintaining genome integrity in human keratinocytes.

  7. Low level anti-Hu reactivity: a risk marker for small cell lung cancer?

    Science.gov (United States)

    Tsou, Jeffrey A.; Kazarian, Meleeneh; Patel, Ankur; Galler, Janice S.; Laird-Offringa, Ite A.; Carpenter, Catherine L.; London, Stephanie J.

    2010-01-01

    Background Previous experimental and laboratory studies have implicated antibodies against Hu proteins (anti-Hu) as a potential marker for small cell lung cancer (SCLC); there are no estimates of the association between anti-Hu and SCLC using a population-based design. Methods We used stored plasma specimens to evaluate anti-Hu reactivity in relationship to small cell lung cancer in a population-based case-control study. Using Western Blot analysis, we measured anti-Hu reactivity against recombinant Hu family member, HuD, in plasma samples from forty-one (41) SCLC cases and seventy-nine (79) controls individually matched for age, race, sex, and smoking status (never, past, current). We analyzed the association between anti-Hu reactivity and SCLC using conditional logistic regression. Results Anti-Hu reactivity was associated with SCLC, both before and after adjustment for amount of smoking. We observed a smoking-adjusted odds ratio of 3.2 (95 % confidence interval from 0.98 to 13.4) comparing subjects above 1800 units (the lower limit of the second tertile of the distribution among antibody positive controls) to subjects with lower reactivity. We also found suggestive evidence in follow-up of our cases that anti-Hu above 1800 units was related to longer-term survival from SCLC. The present research is the first report of anti-Hu reactivity and SCLC in a population-based study. Conclusions Given the suggestive evidence in this study, prospective analyses to examine whether anti-Hu reactivity might predict risk of developing SCLC, or whether anti-Hu reactivity could serve as an early marker for SCLC, may be warranted. PMID:19070439

  8. Administrative Circular N° 26 (Rev.  5) - November 2004

    CERN Multimedia

    Human Resources Department

    2004-01-01

    Procedure governing the career evolution of staff members The introduction of an electronic individual appraisal report form via EDH for the MAPS exercise entails some modifications to Administrative Circular N° 26 (Rev. 4). The revised version (Rev. 5) is available in departmental secretariats as well as on the Web at the following address: http://cern.ch/hr-web/internal/admin_services/admincirc/listadmincirc.asp Human Resources Department Tel. 74128

  9. IHF and HU: flexible architects of bent DNA.

    Science.gov (United States)

    Swinger, Kerren K; Rice, Phoebe A

    2004-02-01

    The energetic cost of bending short segments of DNA is very high. This bending is critical for the packaging of DNA and is exploited to regulate many cellular processes. In prokaryotes, IHF and HU are key architectural proteins present at high concentrations. New protein-DNA co-crystal structures, and the adaptation of advanced biophysical and biochemical techniques have led to an improved understanding of how these proteins interact with DNA. These techniques include time-resolved synchrotron X-ray footprinting, differential scanning calorimetry, isothermal titration calorimetry and single-molecule experiments.

  10. Pärnu REV sai kuuekümneseks / Andres Mets

    Index Scriptorium Estoniae

    Mets, Andres, 1943-

    2004-01-01

    Ehitusfirma Pärnu REV tähistab ainsa toimiva ja sisu muutnud remondi- ja ehitusvalitsusena kuuekümneaastast juubelit. Kommenteerivad REV-i nõukogu esimees Raivo Pulk ja REV-i juhataja Uno Kõressaar

  11. Pärnu REV sai kuuekümneseks / Andres Mets

    Index Scriptorium Estoniae

    Mets, Andres, 1943-

    2004-01-01

    Ehitusfirma Pärnu REV tähistab ainsa toimiva ja sisu muutnud remondi- ja ehitusvalitsusena kuuekümneaastast juubelit. Kommenteerivad REV-i nõukogu esimees Raivo Pulk ja REV-i juhataja Uno Kõressaar

  12. HuPSON: the human physiology simulation ontology.

    Science.gov (United States)

    Gündel, Michaela; Younesi, Erfan; Malhotra, Ashutosh; Wang, Jiali; Li, Hui; Zhang, Bijun; de Bono, Bernard; Mevissen, Heinz-Theodor; Hofmann-Apitius, Martin

    2013-11-22

    Large biomedical simulation initiatives, such as the Virtual Physiological Human (VPH), are substantially dependent on controlled vocabularies to facilitate the exchange of information, of data and of models. Hindering these initiatives is a lack of a comprehensive ontology that covers the essential concepts of the simulation domain. We propose a first version of a newly constructed ontology, HuPSON, as a basis for shared semantics and interoperability of simulations, of models, of algorithms and of other resources in this domain. The ontology is based on the Basic Formal Ontology, and adheres to the MIREOT principles; the constructed ontology has been evaluated via structural features, competency questions and use case scenarios.The ontology is freely available at: http://www.scai.fraunhofer.de/en/business-research-areas/bioinformatics/downloads.html (owl files) and http://bishop.scai.fraunhofer.de/scaiview/ (browser). HuPSON provides a framework for a) annotating simulation experiments, b) retrieving relevant information that are required for modelling, c) enabling interoperability of algorithmic approaches used in biomedical simulation, d) comparing simulation results and e) linking knowledge-based approaches to simulation-based approaches. It is meant to foster a more rapid uptake of semantic technologies in the modelling and simulation domain, with particular focus on the VPH domain.

  13. Adaptive and maladaptive expression of the mRNA regulatory protein HuR

    Institute of Scientific and Technical Information of China (English)

    Suman; Govindaraju; Beth; S; Lee

    2013-01-01

    The RNA-binding proteins involved in regulation of mRNA post-transcriptional processing and translation control the fates of thousands of mRNA transcripts and basic cellular processes. The best studied of these, HuR, is well characterized as a mediator of mRNA stability and translation, and more recently, as a factor in nuclear functions such as pre-mRNA splicing. Due to HuR’s role in regulating thousands of mRNA transcripts, including those for other RNA-binding proteins, HuR can act as a master regulator of cell survival and proliferation. HuR itself is subject to multiple post-translationa modifications including regulation of its nucleocytoplasmic distribution. However, the mechanisms that govern HuR levels in the cell have only recently begun to be defined. These mechanisms are critical to cell health, as it has become clear in recent years that aberrant expression of HuR can lead alternately to decreased cell viability or to promotion of pathological proliferation and invasiveness. HuR is expressed as alternate mRNAs that vary in their untranslated regions, leading to differences in transcript stability and translatability. Multiple transcription factors and modulators of mRNA stability that regulate HuR mRNA expression have been identified. In addition, translation of HuR is regulated by numerous microRNAs, several of which have been demonstrated to have anti-tumor properties due to their suppression of HuR expression. This review summarizes the current state of knowledge of the factors that regulate HuR expression, along with the circumstances under which these factors contribute to cancer and inflammation.

  14. Fingerprint Recognition Using Local Features and Hu Moments

    Directory of Open Access Journals (Sweden)

    G. Aguilar-Torres

    2012-10-01

    Full Text Available Person identification systems based on fingerprint patterns called Automatic Fingerprint Identification Systems, AFIS,are some of the most widely used biometric methods since they provide a high degree of success. The accuracy ofAFIS is mainly due to some unique characteristics called minutiae, which are points where a curve track finishes,intersects with another curve track, or branches off. During past decades several efficient minutia-based fingerprintrecognition algorithms have been proposed which achieve false recognition rates close to 1%, however, theirrecognition rate may be still improved. To this end, this paper presents a fingerprint recognition method using acombination of the Fast Fourier Transform (FFT with Gabor filters for image enhancement. Next, fingerprintrecognition is carried out using a novel recognition stage based on Local Features and Hu invariant moments forverification.

  15. HIV-1 tat and rev upregulates osteoclast bone resorption

    Directory of Open Access Journals (Sweden)

    Nicholas Chew

    2014-11-01

    Full Text Available Introduction: Disruption in bone homeostasis with increased osteoclastic resorption may lead to osteoporosis. HIV tat has been found to increase differentiation of precursor cells into osteoclast (OC (1. Presence of soluble HIV proteins in virally suppressed HIV patients on ART may drive a bone resorption phenotype. We investigated the role of soluble HIV proteins (tat, gp120 Mn and Bal, rev and p55-gag on osteoclastogenesis and OC resorptive capacity. Methods: Mouse monocyte RAW 264.7 cells were cultured in vitro and induced to differentiate into OCs with 50 ng/mL RANKL and 25 ng/mL mCSF. Medium was supplemented with 100 ng/mL of recombinant HIV tat, gp120 (Mn and Bal, rev, nef and p55-gag, respectively, with zolendronate as negative control. Differentiated OCs were stained for TRAP and counted. OC resorption function was examined by culturing differentiated OCs (in the presence of respective HIV proteins on dentin-coated plates and examining the following (i sealing zone formation, (ii volume of resorption pits and (iii area of resorption pits per field using confocal microscopy. Expression of OC specific genes including NFATc1 and cathepsin K was investigated by qPCR. Reactive oxygen species (ROS production is essential in RANKL-induced OC differentiation (2,3; effect of these proteins on ROS production was assessed using the fluorescent H2DCFH-DA. Mean fluorescence intensity was then measured by flow cytometry. TNFα production by OC precursors when incubated with tat and rev was measured by ELISA. Results: Tat and rev treatment was associated with increased OC formation by 70 and 26%, respectively (p<0.01, relative to control, while zolendronate significantly inhibited OC formation by 75%. Gp120 Mn and Bal, nef and p55-gag treatment had no effect on OC differentiation. Interestingly, neither tat nor rev treatment caused significant increases in sealing zone formation but increased dentin resorption pit area by 28 and 19%, respectively, and

  16. Administrative Circular No. 26 (Rev. 9) – Recognition of Merit

    CERN Multimedia

    2012-01-01

    Administrative Circular No. 26 (Rev. 9) entitled "Recognition of Merit”, approved by the Director-General following discussion in the Standing Concertation Committee meeting on 27 September 2011 is available on the intranet site of the Human Resources Department: https://cern.ch/hr-docs/admincirc/admincirc.asp The circular was above all revised in order to integrate the new CERN Competency Model into the annual procedure of performance appraisal. It cancels and replaces Administrative Circular No. 26 (Rev. 8) entitled "Recognition of merit” of September 2008. Department Head Office HR Department

  17. Administrative Circular No. 26 (Rev. 7) – May 2007

    CERN Multimedia

    HR Department

    2007-01-01

    Recognition of Merit of Staff Members Administrative Circular No. 26 (Rev. 7) is now available on the intranet site of the Human Resources Department. This circular cancels and replaces Administrative Circular No. 26 (Rev. 6) - Procedures governing the career development of staff members. Copies will shortly be available in Departmental secretariats. If you require any additional information on the new staff-member merit assessment and recognition system, you may consult the FAQ, which has been available on the Human Resources Department intranet site since February 2007. Human Resources Department Tel. 78003

  18. “Meticulous Crafting” and “Plain Style”: Hu Shi's Prose

    Institute of Scientific and Technical Information of China (English)

    ChenPingyuan; ZhuShida; DavidKelly

    2004-01-01

    Hu Shi's Outline History of Chinese Philosophy, despite a number of shortcomings, opened a new era for an academic prose in vernacular Chinese. Hu set great store by the structural features of a """"work,"""" arguing that it was not enough to assemble quotes,

  19. A Meta Analysis and Hierarchical Classification of HU-Based Atherosclerotic Plaque Characterization Criteria

    NARCIS (Netherlands)

    Kristanto, Wisnumurti; van Ooijen, Peter M. A.; Jansen-van der Weide, Marijke C.; Vliegenthart, Rozemarijn; Oudkerk, Matthijs

    2013-01-01

    Background: Many computed tomography (CT) studies have reported that lipid-rich, presumably rupture-prone atherosclerotic plaques can be characterized according to their Hounsfield Unit (HU) value. However, the published HU-based characterization criteria vary considerably. The present study aims to

  20. HuD Regulates Coding and Noncoding RNA to Induce APP→Aβ Processing

    Directory of Open Access Journals (Sweden)

    Min-Ju Kang

    2014-06-01

    Full Text Available The primarily neuronal RNA-binding protein HuD is implicated in learning and memory. Here, we report the identification of several HuD target transcripts linked to Alzheimer’s disease (AD pathogenesis. HuD interacted with the 3′ UTRs of APP mRNA (encoding amyloid precursor protein and BACE1 mRNA (encoding β-site APP-cleaving enzyme 1 and increased the half-lives of these mRNAs. HuD also associated with and stabilized the long noncoding (lncRNA BACE1AS, which partly complements BACE1 mRNA and enhances BACE1 expression. Consistent with HuD promoting production of APP and APP-cleaving enzyme, the levels of APP, BACE1, BACE1AS, and Aβ were higher in the brain of HuD-overexpressing mice. Importantly, cortex (superior temporal gyrus from patients with AD displayed significantly higher levels of HuD and, accordingly, elevated APP, BACE1, BACE1AS, and Aβ than did cortical tissue from healthy age-matched individuals. We propose that HuD jointly promotes the production of APP and the cleavage of its amyloidogenic fragment, Aβ.

  1. Comment on “A study of vertical and in-plane electron mobility due to interface roughness scattering at low temperature in InAs-GaSb superlattices” [J. Appl. Phys. 114, 053712 (2013)

    Energy Technology Data Exchange (ETDEWEB)

    Szmulowicz, F., E-mail: Frank.Szmulowicz.ctr@wpafb.af.mil [University of Dayton Research Institute, 300 College Park Ave., Dayton, Ohio 45469-0072 (United States)

    2014-04-14

    The purpose of this comment is to point out that the paper by Safa, Asgari, and Faraone [J. Appl. Phys. 114, 053712 (2013)] (SAF) on electronic transport in superlattices contains a number of errors in physics and execution. By dealing with a finite number of periods and forcing the wave function to be zero at the upper and lower boundaries of the superlattice stack, SAF have turned the system into a quantum well for which the momentum along the growth axis is not a good quantum number, so that the bands in the growth direction are flat and the corresponding carrier velocities and vertical mobilities are zero. A number of other errors allow the authors to get nonzero results and to reach conclusions that qualitatively mirror those of Szmulowicz, Haugan, Elhamri, and Brown [Phys. Rev. B 84, 155307 (2011)].

  2. Comment on "A study of vertical and in-plane electron mobility due to interface roughness scattering at low temperature in InAs-GaSb superlattices" [J. Appl. Phys. 114, 053712 (2013)

    Science.gov (United States)

    Szmulowicz, F.

    2014-04-01

    The purpose of this comment is to point out that the paper by Safa, Asgari, and Faraone [J. Appl. Phys. 114, 053712 (2013)] (SAF) on electronic transport in superlattices contains a number of errors in physics and execution. By dealing with a finite number of periods and forcing the wave function to be zero at the upper and lower boundaries of the superlattice stack, SAF have turned the system into a quantum well for which the momentum along the growth axis is not a good quantum number, so that the bands in the growth direction are flat and the corresponding carrier velocities and vertical mobilities are zero. A number of other errors allow the authors to get nonzero results and to reach conclusions that qualitatively mirror those of Szmulowicz, Haugan, Elhamri, and Brown [Phys. Rev. B 84, 155307 (2011)].

  3. Immune response in lung cancer mouse model mimics human anti-Hu reactivity

    Science.gov (United States)

    Kazarian, Meleeneh; Calbo, Joaquim; Proost, Natalie; Carpenter, Catherine L.; Berns, Anton; Laird-Offringa, Ite A.

    2009-01-01

    Most patients with paraneoplastic encephalomyelitis/sensory neuronopathy PEM/SN have small-cell lung cancer (SCLC) and develop antibodies against neuronal-specific Hu proteins, which are abnormally expressed in the tumor. Anti-Hu reactivity is present in ~16% of SCLC patients without PEM/SN. Here we test the hypothesis that engineered SCLC-prone mice may exhibit anti-Hu reactivity. We show that tumors from SCLC-prone mice misexpress Hu proteins, and 14% of mice harbor anti-Hu antibodies. Mice appear to show reactivity prior to clinical diagnosis of SCLC. This mouse model system will be useful to study SCLC-associated autoimmunity, its diagnostic value, and the potential protective role of oncoantigen-directed autoantibodies. PMID:19765830

  4. Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel insights into the regulation of Rev nuclear import.

    LENUS (Irish Health Repository)

    Gu, Lili

    2011-01-01

    The HIV-1 regulatory protein Rev, which is essential for viral replication, mediates the nuclear export of unspliced viral transcripts. Rev nuclear function requires active nucleocytoplasmic shuttling, and Rev nuclear import is mediated by the recognition of its Nuclear Localisation Signal (NLS) by multiple import factors, which include transportin and importin β. However, it remains unclear which nuclear import pathway(s) predominate in vivo, and the cellular environment that modulates Rev nucleocytoplasmic shuttling remains to be characterised.

  5. HuR knockdown changes the oncogenic potential of oral cancer cells.

    Science.gov (United States)

    Kakuguchi, Wataru; Kitamura, Tetsuya; Kuroshima, Takeshi; Ishikawa, Makoto; Kitagawa, Yoshimasa; Totsuka, Yasunori; Shindoh, Masanobu; Higashino, Fumihiro

    2010-04-01

    HuR binds to AU-rich element-containing mRNA to protect them from rapid degradation. Here, we show that knockdown of HuR changes the oncogenic properties of oral cancer cells. Oral squamous cell carcinoma cell lines, HSC-3 and Ca9.22, which express HuR protein and cytoplasmic AU-rich element mRNA more abundantly than normal cells, were subjected to HuR knockdown. In the HuR-knockdown cancer cells, the cytoplasmic expression of c-fos, c-myc, and COX-2 mRNAs was inhibited compared with those in cells that had been transfected with a control small interfering RNA, and the half-lives of these mRNAs were shorter than those of their counterparts in the control cells. HuR-knockdown cells failed to make colonies in soft agar, suggesting that the cells had lost their ability for anchorage-independent cell growth. Additionally, the motile and invasive activities of the cells decreased remarkably by HuR knockdown. Furthermore, the expression of cell cycle-related proteins, such as cyclin A, cyclin B1, cyclin D1, and cyclin-dependent kinase 1, was reduced in HuR-knockdown cancer cells, and HuR bound to cdk1 mRNA to stabilize it. These findings suggest that HuR knockdown changes the features of oral cancer cells, at least in part, by affecting their cell cycle and shows potential as an effective therapeutic approach.

  6. HuR contributes to Hepatic Stellate Cell activation and liver fibrosis

    Science.gov (United States)

    Woodhoo, A.; Iruarrizaga-Lejarreta, M.; Beraza, N.; García-Rodríguez, J.L.; Embade, N.; Fernández-Ramos, D.; Matinez-Lopez, N.; Gutiérrez, Virginia; Arteta, B.; Caballeria, J.; Lu, S.C.; Mato, J.M.; Varela-Rey, M.; Martinez-Chantar, M.L.

    2012-01-01

    RNA-binding proteins (RBPs) play a major role in control of mRNA turnover and translation rates. We examined the role of the RBP human antigen R (HuR) during cholestatic liver injury and hepatic stellate cells (HSC) activation. HuR silencing attenuated fibrosis development in vivo after BDL, reducing liver damage, oxidative stress, inflammation, and collagen and α-SMA (α-smooth muscle actin) expression. HuR expression increased in activated HSC from BDL mice and during HSC activation in vitro, and HuR silencing markedly reduced HSC activation. HuR regulated platelet-derived growth factor (PDGF)-induced proliferation and migration, and controlled expression of several mRNAs involved in these processes (Actin, MMP9, Cyclin D1 and B1). These functions of HuR were linked to its abundance and cytoplasmic localisation, controlled by PDGF, via ERK and PI3K activation, and ERK-LKB1 activation respectively. More importantly, we identified the tumor suppressor LKB1 as a novel downstream target of PDGF-induced ERK activation in HSC. HuR also controlled transforming growth factor beta (TGF-β-induced profibrogenic actions by regulating expression of TGF-β, α-SMA, and p21. This was likely due to an increased cytoplasmic localisation of HuR, controlled by TGF-β-induced p38 MAPK activation. Finally, we found that HuR and LKB1 (Ser428) levels were highly expressed in activated HSC in human cirrhotic samples. Conclusion: Our results show that HuR is important for pathogenesis of liver fibrosis development in the cholestatic injury model, for HSC activation, and for the response of activated HSC to PDGF and TGF-β. PMID:22576182

  7. Wind Farm Wake: The Horns Rev Photo Case

    DEFF Research Database (Denmark)

    Hasager, Charlotte Bay; Rasmussen, Leif; Peña, Alfredo

    2013-01-01

    The aim of the paper is to examine the nowadays well-known wind farm wake photographs taken on 12 February 2008 at the offshore Horns Rev 1 wind farm. The meteorological conditions are described from observations from several satellite sensors quantifying clouds, surface wind vectors and sea...

  8. Revised Safety Instruction NO. 4 (IS4 REV.)

    CERN Multimedia

    2006-01-01

    Please note that the revised safety instruction no. 4 (IS4 REV.), entitled 'Safety Inspections' is available on the web at the following url: https://edms.cern.ch/file/335741/LAST_RELEASED/E_IS4.pdf Paper copies can be obtained from the SC unit secretariat. SC Secretariat e-mail: sc.secretariat@cern.ch

  9. Revised Safety Instruction NO. 4 (IS4 REV.)

    CERN Multimedia

    SC Secretariat

    2006-01-01

    Please note that the revised safety instruction No. 4 (IS4 REV.), entitled 'Safety Inspections' is available on the web at the following url: https://edms.cern.ch/file/335741/LAST_RELEASED/E_IS4.pdf Paper copies can be obtained from the SC unit secretariat. SC Secretariat e-mail: sc.secretariat@cern.ch

  10. Revised Safety Instruction NO. 4 (IS4 REV.)

    CERN Multimedia

    2006-01-01

    Please note that the revised safety instruction no. 4 (IS4 REV.), entitled 'Safety Inspections' is available on the web at the following url: https://edms.cern.ch/file/335741/LAST_RELEASED/E_IS4.pdf Paper copies can be obtained from the SC unit secretariat. SC Secretariat sc.secretariat@cern.ch

  11. Wind Farm Wake: The 2016 Horns Rev Photo Case

    DEFF Research Database (Denmark)

    Hasager, Charlotte Bay; Nygaard, Nicolai Gayle; Volker, Patrick

    2017-01-01

    Offshore wind farm wakes were observed and photographed in foggy conditions at Horns Rev 2 on 25 January 2016 at 12:45 UTC. These new images show highly contrasting conditions regarding the wind speed, turbulence intensity, atmospheric stability, weather conditions and wind farm wake development...

  12. Regulation of the mRNA-binding protein HuR by posttranslational modification: spotlight on phosphorylation.

    Science.gov (United States)

    Eberhardt, Wolfgang; Doller, Anke; Pfeilschifter, Josef

    2012-06-01

    The ubiquitous mRNA-binding protein human antigen R (HuR) and its neuronal relatives (HuB, HuC, HuD) participate in the post-transcriptional regulation of many AU-rich element-bearing mRNAs. In addition to its originally described role in controlling mRNA decay, the binding of HuR to target mRNAs can affect many aspects of mRNA processing including splicing, polyadenylation, intracellular trafficking, translation and modulation of mRNA repression by miRNAs. In accordance to the growing list of signalling events which are involved in regulating these different HuR functions, recent data implicate that posttranslational modification, namely protein kinase-triggered phosphorylation of HuR plays a crucial role in connecting extracellular signal inputs to a specific post-transcriptional program by HuR. Notably, in addition to directly targeting HuR functions, posttranslational modifications of HuR have a major impact on the sequestration and binding to various HuR ligand proteins as has been demonstrated e.g. for the 14-3-3 chaperones. However, the detailed mechanisms of how a specific modification of HuR coordinates different aspects in HuR regulation are currently poorly understood. Due to the fact that most of the described HuR activities are closely related to its subcellular localization and the binding to cargo mRNA, this review will focus on these aspects of HuR functions and their control by posttranslational modification, particularly by HuR phosphorylations by different protein kinases.

  13. Structural characterization of the maytansinoid–monoclonal antibody immunoconjugate, huN901–DM1, by mass spectrometry

    National Research Council Canada - National Science Library

    Wang, Lintao; Amphlett, Godfrey; Blättler, Walter A; Lambert, John M; Zhang, Wei

    2005-01-01

    ...‐specific drug delivery. The immunoconjugate, huN901–DM1, composed of the humanized monoclonal IgG 1 antibody, huN901, and the maytansinoid drug, DM1, is being tested in clinical trials to treat small cell lung carcinoma (SCLC). huN901...

  14. Measurement of hard tissue density of head phantom based on the HU by using CBCT

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Moon Sun; Kang, Dong Wan; Kim, Jae Duk [School of Dentistry, Chosun University, Gwangju (Korea, Republic of)

    2009-09-15

    The purpose of this study was to determine a conversion coefficient for Hounsfield Units(HU) to material density (g cm{sup -3}) obtained from cone-beam computed tomography (CBMercuRay{sup TM}) data and to measure the hard tissue density based on the Hounsfield scale on dental head phantom. CT Scanner Phantom (AAPM) equipped with CT Number Insert consists of five cylindrical pins of materials with different densities and teflon ring was scanned by using the CBMercuRay{sup TM} (Hitachi, Tokyo, Japan) volume scanner. The raw data were converted into DICOM format and the HU of different areas of CT number insert measured by using CBWorks{sup TM}. Linear regression analysis and Student t-test were performed statistically. There was no significant difference (P>0.54) between real densities and measured densities. A linear regression was performed using the density, {rho} (g cm{sup -3}), as the dependent variable in terms of the HU (H). The regression equation obtained was {rho}=0.00072 H-0.01588 with an R2 value of 0.9968. Density values based on the Hounsfield scale was 1697.1 {+-} 24.9 HU in cortical bone, 526.5 {+-} 44.4 HU in trabecular bone, 2639.1 {+-} 48.7 HU in enamel, 1246.1 {+-} 39.4 HU in dentin of dental head phantom. CBCT provides an effective option for determination of material density expressed as Hounsfield Units.

  15. On the ephemeris of the eclipsing polar HU Aquarii

    CERN Document Server

    Schwope, A D

    2014-01-01

    The magnetic cataclysmic variable HU Aquarii displayed pronounced quasi-periodic modulations of its eclipse timing. These were interpreted in terms of the light-travel time (LTT) effect caused by a circumbinary planet or planetary system. We report new photometric observations that revealed another precise eclipse timing for the October 2013 epoch, the first obtained in a high accretion state after many years in low or intermediate states. The eclipse was observed to occur earlier by 95.3 +- 2.0 s or 62.8 +- 2.0 s than expected for an assumed linear or quadratic ephemeris, respectively. The implied apparent strong evolution of the orbital period calls for a revision of the current planetary model or the planetary parameters. The object deserves further monitoring to uncover the true nature of the observed variability and to constrain the properties of the proposed planet or planetary system. The new observations prove that advanced amateur equipment can successfully be used in the growing field of planet sear...

  16. Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of Rev nuclear import.

    LENUS (Irish Health Repository)

    Gu, Lili

    2011-03-14

    Abstract Background The HIV-1 regulatory protein Rev, which is essential for viral replication, mediates the nuclear export of unspliced viral transcripts. Rev nuclear function requires active nucleocytoplasmic shuttling, and Rev nuclear import is mediated by the recognition of its Nuclear Localisation Signal (NLS) by multiple import factors, which include transportin and importin β. However, it remains unclear which nuclear import pathway(s) predominate in vivo, and the cellular environment that modulates Rev nucleocytoplasmic shuttling remains to be characterised. Results In our study, we have identified the cellular protein HIC (Human I-mfa domain-Containing protein) as a novel interactor of HIV-1 Rev. We demonstrate that HIC selectively interferes with Rev NLS interaction with importin β and impedes its nuclear import and function, but does not affect Rev nuclear import mediated by transportin. Hence, the molecular determinants mediating Rev-NLS recognition by importin β and transportin appear to be distinct. Furthermore, we have employed HIC and M9 M, a peptide specifically designed to inhibit the transportin-mediated nuclear import pathway, to characterise Rev nuclear import pathways within different cellular environments. Remarkably, we could show that in 293T, HeLa, COS7, Jurkat, U937, THP-1 and CEM cells, Rev nuclear import is cell type specific and alternatively mediated by transportin or importin β, in a mutually exclusive fashion. Conclusions Rev cytoplasmic sequestration by HIC may represent a novel mechanism for the control of Rev function. These studies highlight that the multivalent nature of the Rev NLS for different import receptors enables Rev to adapt its nuclear trafficking strategy.

  17. Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of Rev nuclear import

    Directory of Open Access Journals (Sweden)

    Sheehy Noreen

    2011-03-01

    Full Text Available Abstract Background The HIV-1 regulatory protein Rev, which is essential for viral replication, mediates the nuclear export of unspliced viral transcripts. Rev nuclear function requires active nucleocytoplasmic shuttling, and Rev nuclear import is mediated by the recognition of its Nuclear Localisation Signal (NLS by multiple import factors, which include transportin and importin β. However, it remains unclear which nuclear import pathway(s predominate in vivo, and the cellular environment that modulates Rev nucleocytoplasmic shuttling remains to be characterised. Results In our study, we have identified the cellular protein HIC (Human I-mfa domain-Containing protein as a novel interactor of HIV-1 Rev. We demonstrate that HIC selectively interferes with Rev NLS interaction with importin β and impedes its nuclear import and function, but does not affect Rev nuclear import mediated by transportin. Hence, the molecular determinants mediating Rev-NLS recognition by importin β and transportin appear to be distinct. Furthermore, we have employed HIC and M9 M, a peptide specifically designed to inhibit the transportin-mediated nuclear import pathway, to characterise Rev nuclear import pathways within different cellular environments. Remarkably, we could show that in 293T, HeLa, COS7, Jurkat, U937, THP-1 and CEM cells, Rev nuclear import is cell type specific and alternatively mediated by transportin or importin β, in a mutually exclusive fashion. Conclusions Rev cytoplasmic sequestration by HIC may represent a novel mechanism for the control of Rev function. These studies highlight that the multivalent nature of the Rev NLS for different import receptors enables Rev to adapt its nuclear trafficking strategy.

  18. HuR contributes to Hepatic Stellate Cell activation and liver fibrosis

    OpenAIRE

    Woodhoo, A.; Iruarrizaga-Lejarreta, M.; Beraza, N.; García-Rodríguez, J.L.; Embade, N.; Fernández-Ramos, D.; Matinez-Lopez, N.; Gutiérrez, Virginia; Arteta, B; Caballeria, J.; Lu, S.C. (Shelly C.); Mato, J.M. (José María); Varela-Rey, M.; Martinez-Chantar, M.L.

    2012-01-01

    RNA-binding proteins (RBPs) play a major role in control of mRNA turnover and translation rates. We examined the role of the RBP human antigen R (HuR) during cholestatic liver injury and hepatic stellate cells (HSC) activation. HuR silencing attenuated fibrosis development in vivo after BDL, reducing liver damage, oxidative stress, inflammation, and collagen and α-SMA (α-smooth muscle actin) expression. HuR expression increased in activated HSC from BDL mice and during HSC activation in vitro...

  19. Administrative Circular No. 26 (Rev.10) - Recognition of merit

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 26 (Rev. 10) entitled “Recognition of Merit”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 5 December 2013 and entering into force on 1 January 2014, is available on the intranet site of the Human Resources Department (see here).   This circular is applicable to staff members. It cancels and replaces Administrative Circular No. 26 (Rev. 9) entitled “Recognition of Merit” of December 2011. The circular was revised in order to take into account the work performed in the framework of an elective mandate during the exercise of merit recognition of staff members. In addition, the circular was revised to provide that, in the case of staff members on special leave for professional reasons for a period equal to or longer than half a year, it will no longer be possible to grant an exceptional advancement. Department Head Office HR Department

  20. Administrative Circular No. 23 (Rev. 4) - Special working hours

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 23 (Rev. 4) entitled "Special working hours", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 22 March 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208539.   This revised circular cancels and replaces Administrative Circular No. 23 (Rev. 3) also entitled "Special working hours" of January 2013. This document contains modifications to reflect the new career structure and ensuring the provision consistent with practice that compensation or remuneration of special working hours performed remotely is possible only in case of emergency.   This circular will enter into force on 1st September 2016.

  1. Operational circular No. 1 (Rev. 1) – Operational circulars

    CERN Multimedia

    HR Department

    2011-01-01

    Operational Circular No. 1 (Rev. 1) is applicable to members of the personnel and other persons concerned. Operational Circular No. 1 (Rev. 1) entitled "Operational circulars", approved following discussion at the Standing Concertation Committee meeting on 4 May 2011, is available on the intranet site of the Human Resources Department: https://hr-docs.web.cern.ch/hr-docs/opcirc/opcirc.asp It cancels and replaces Operational Circular No. 1 entitled "Operational Circulars” of December 1996. This new version clarifies, in particular, that operational circulars do not necessarily arise from the Staff Rules and Regulations, and the functional titles have been updated to bring them into line with the current CERN organigram. Department Head Office  

  2. Safety Instruction nº 23 (IS 23 Rev. 3)

    CERN Multimedia

    SC Secretariat

    2005-01-01

    Please note that the revised version of Safety Instruction no 23 (IS 23 rev. 3) entitled "Criteria and standard test methods for the selection of electric cables and wires with respect to fire safety and radiation resistance" is available on the web at the following url: https://edms.cern.ch/document/335745/LAST_RELEASED/ Paper copies can also be obtained from the SC Secretariat, e-mail: sc.secretariat@cern.ch SC Secretariat

  3. International Field Reversible Thermal Connector (RevCon) Challenge

    Science.gov (United States)

    2016-07-01

    AFRL-RY-WP-TR-2016-0108 INTERNATIONAL FIELD REVERSIBLE THERMAL CONNECTOR (RevCon) CHALLENGE Chung-Lung Chen University of...or corporation; or convey any rights or permission to manufacture, use, or sell any patented invention that may relate to them. This report is...DD-MM-YY) 2. REPORT TYPE 3. DATES COVERED (From - To) July 2016 Final 07 November 2013 – 15 March 2016 4. TITLE AND SUBTITLE INTERNATIONAL

  4. REV-ERB and ROR: therapeutic targets for treating myopathies

    Science.gov (United States)

    Welch, Ryan D.; Flaveny, Colin A.

    2017-08-01

    Muscle is primarily known for its mechanical roles in locomotion, maintenance of posture, and regulation of cardiac and respiratory function. There are numerous medical conditions that adversely affect muscle, myopathies that disrupt muscle development, regeneration and protein turnover to detrimental effect. Skeletal muscle is also a vital secretory organ that regulates thermogenesis, inflammatory signaling and directs context specific global metabolic changes in energy substrate preference on a daily basis. Myopathies differ in the causative factors that drive them but share common features including severe reduction in quality of life and significantly increased mortality all due irrefutably to the loss of muscle mass. Thus far clinically viable approaches for preserving muscle proteins and stimulating new muscle growth without unwanted side effects or limited efficacy has been elusive. Over the last few decades, evidence has emerged through in vitro and in vivo studies that suggest the nuclear receptors REV-ERB and ROR might modulate pathways involved in myogenesis and mitochondrial biogenesis. Hinting that REV-ERB and ROR might be targeted to treat myopathies. However there is still a need for substantial investigation into the roles of these nuclear receptors in in vivo rodent models of degenerative muscle diseases and acute injury. Although exciting, REV-ERB and ROR have somewhat confounding roles in muscle physiology and therefore more studies utilizing in vivo models of skeletal muscle myopathies are needed. In this review we highlight the molecular forces driving some of the major degenerative muscular diseases and showcase two promising molecular targets that may have the potential to treat myopathies: ROR and REV-ERB.

  5. RevPAR、GOP率、GOPPAR的联动增长

    Institute of Scientific and Technical Information of China (English)

    陈雪羽

    2007-01-01

    RevPAR,指平均每间可售客房产生的收入,其计算方式是酒店客房的全年营收总额除以全年可供出售的客房总间数,或者用更简化的方式,就是用平均房价乘以平均出租率。

  6. Ms HU Ling-xiang's Experience in Treating Blood Stasis by Acupuncture Balancing Method

    Institute of Scientific and Technical Information of China (English)

    FU Jia; LI Ji; ZHANG Cai-rong

    2005-01-01

    @@ HU Ling-xiang, 60, is a chief doctor and supervisor of postgraduates of master degree. She specializes in the syndrome differentiation and treatment of different kinds of chronic and difficult disorders from the perspective of blood stasis theory.

  7. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export.

    Science.gov (United States)

    Behrens, Ryan T; Aligeti, Mounavya; Pocock, Ginger M; Higgins, Christina A; Sherer, Nathan M

    2017-02-01

    HIV-1's Rev protein forms a homo-oligomeric adaptor complex linking viral RNAs to the cellular CRM1/Ran-GTP nuclear export machinery through the activity of Rev's prototypical leucine-rich nuclear export signal (NES). In this study, we used a functional fluorescently tagged Rev fusion protein as a platform to study the effects of modulating Rev NES identity, number, position, or strength on Rev subcellular trafficking, viral RNA nuclear export, and infectious virion production. We found that Rev activity was remarkably tolerant of diverse NES sequences, including supraphysiological NES (SNES) peptides that otherwise arrest CRM1 transport complexes at nuclear pores. Rev's ability to tolerate a SNES was both position and multimerization dependent, an observation consistent with a model wherein Rev self-association acts to transiently mask the NES peptide(s), thereby biasing Rev's trafficking into the nucleus. Combined imaging and functional assays also indicated that NES masking underpins Rev's well-known tendency to accumulate at the nucleolus, as well as Rev's capacity to activate optimal levels of late viral gene expression. We propose that Rev multimerization and NES masking regulates Rev's trafficking to and retention within the nucleus even prior to RNA binding.

  8. Zebrafish transgenic line huORFZ is an effective living bioindicator for detecting environmental toxicants.

    Directory of Open Access Journals (Sweden)

    Hung-Chieh Lee

    Full Text Available Reliable animal models are invaluable for monitoring the extent of pollution in the aquatic environment. In this study, we demonstrated the potential of huORFZ, a novel transgenic zebrafish line that harbors a human upstream open reading frame of the chop gene fused with GFP reporter, as an animal model for monitoring environmental pollutants and stress-related cellular processes. When huORFZ embryos were kept under normal condition, no leaked GFP signal could be detected. When treated with hazardous chemicals, including heavy metals and endocrine-disrupting chemicals near their sublethal concentrations (LC50, huORFZ embryos exhibited different tissue-specific GFP expression patterns. For further analysis, copper (Cu2+, cadmium (Cd2+ and Chlorpyrifos were applied. Cu2+ triggered GFP responses in skin and muscle, whereas Cd2+ treatment triggered GFP responses in skin, olfactory epithelium and pronephric ducts. Moreover, fluorescence intensity, as exhibited by huORFZ embryos, was dose-dependent. After surviving treated embryos were returned to normal condition, survival rates, as well as TUNEL signals, returned to pretreatment levels with no significant morphological defects observed. Such results indicated the reversibility of treatment conditions used in this study, as long as embryos survived such conditions. Notably, GFP signals decreased along with recovery, suggesting that GFP signaling of huORFZ embryos likely reflected the overall physiological condition of the individual. To examine the performance of the huORFZ line under real-world conditions, we placed huORFZ embryos in different river water samples. We found that the huORFZ embryos correctly detected the presence of various kinds of pollutants. Based on these findings, we concluded that such uORFchop-based system can be integrated into a first-line water alarm system monitoring the discharge of hazardous pollutants.

  9. Hu'rthle Cell Thyroid Adenoma with an Eggshell Calcification: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dong Gil; Lee, Sang Kwon; Choe, Mi Sun; Chang, Hyuk Won; Rho, Byung Hak [Dongsan Medical Center, Keimyung University School of Medicine, Daegu (Korea, Republic of)

    2009-09-15

    We report here a case of Hu'rthle cell adenoma with eggshell calcification that presented as a thyroid incidentaloma on ultrasonography (US) in a 58-year-old woman. The mass was hypoechoic with continuous eggshell calcification and intranodular vascularity as seen on gray-scale and power Doppler (PD) US. Hu 'rthle cell adenoma should be considered in the differential diagnosis of a thyroid nodule with eggshell calcification

  10. Neuroprotective effects of the cannabinoid agonist HU210 on retinal degeneration.

    Science.gov (United States)

    Lax, Pedro; Esquiva, Gema; Altavilla, Cesare; Cuenca, Nicolás

    2014-03-01

    Cannabinoids have been demonstrated to exert neuroprotective effects on different types of neuronal insults. Here we have addressed the therapeutic potential of the synthetic cannabinoid HU210 on photoreceptor degeneration, synaptic connectivity and functional activity of the retina in the transgenic P23H rat, an animal model for autosomal dominant retinitis pigmentosa (RP). In P23H rats administered with HU210 (100 μg/kg, i.p.) from P24 to P90, ERG recordings showed an amelioration of vision loss, as compared to vehicle-administered animals. Under scotopic conditions, the maximum a-wave amplitudes recorded at P60 and P90 were higher in HU210-treated animals, as compared to the values obtained in untreated animals. The scotopic b-waves were significantly higher in treated animals than in untreated rats at P30, P60 and P90. This attenuation of visual deterioration correlated with a delay in photoreceptor degeneration and the preservation of retinal cytoarchitecture. HU210-treated animals had 40% more photoreceptors than untreated animals. Presynaptic and postsynaptic elements, as well as the synaptic contacts between photoreceptors and bipolar or horizontal cells, were also preserved in HU210-treated P23H rats. These results indicate that HU210 preserves cone and rod structure and function, together with their contacts with postsynaptic neurons, in P23H rats. These data suggest that cannabinoids are potentially useful to delay retinal degeneration in RP patients.

  11. Pertussis toxin B-oligomer inhibits HIV infection and replication in hu-PBL-SCID mice.

    Science.gov (United States)

    Lapenta, Caterina; Spada, Massimo; Santini, Stefano M; Racca, Sara; Dorigatti, Fernanda; Poli, Guido; Belardelli, Filippo; Alfano, Massimo

    2005-04-01

    Bordetella pertussis toxin B-oligomer (PTX-B) has been shown to inhibit HIV infection and replication in vitro. The potential anti-viral effect of PTX-B was tested here in an in vivo surrogate model of HIV infection, i.e. SCID mice reconstituted with human peripheral blood leukocytes (PBL) (hu-PBL-SCID) and infected with a CCR5-dependent (R5) HIV-1 strain. SCID mice inoculated intra-peritoneal (i.p.) with PTX-B and then infected with the R5 strain SF-162 were sacrificed 7 days later and analyzed for human PBL (hu-PBL) lymphoid tissue reconstitution, infection of hu-PBL, plasma viremia and viral rescue from ex vivo-cultivated i.p. hu-PBL. Unlike mice treated with 500 ng per animal of PTX-B showing no evidence of viral inhibition, daily administration of PTX-B (50 ng per mouse) strongly inhibited virus infection and replication, as determined by undetectable viremia, absence of infected hu-PBL and lack of rescue of infectious HIV in most animals. Furthermore, PTX-B injection 2 h before and twice after infection prevented HIV-1 infection and replication in all (10/10) tested animals. Thus, PTX-B potently inhibited virus infection and replication in hu-PBL-SCID mice, supporting the hypothesis that it may represent a new pharmacological agent against HIV-1 infection.

  12. Different modes of interaction by TIAR and HuR with target RNA and DNA.

    Science.gov (United States)

    Kim, Henry S; Wilce, Matthew C J; Yoga, Yano M K; Pendini, Nicole R; Gunzburg, Menachem J; Cowieson, Nathan P; Wilson, Gerald M; Williams, Bryan R G; Gorospe, Myriam; Wilce, Jacqueline A

    2011-02-01

    TIAR and HuR are mRNA-binding proteins that play important roles in the regulation of translation. They both possess three RNA recognition motifs (RRMs) and bind to AU-rich elements (AREs), with seemingly overlapping specificity. Here we show using SPR that TIAR and HuR bind to both U-rich and AU-rich RNA in the nanomolar range, with higher overall affinity for U-rich RNA. However, the higher affinity for U-rich sequences is mainly due to faster association with U-rich RNA, which we propose is a reflection of the higher probability of association. Differences between TIAR and HuR are observed in their modes of binding to RNA. TIAR is able to bind deoxy-oligonucleotides with nanomolar affinity, whereas HuR affinity is reduced to a micromolar level. Studies with U-rich DNA reveal that TIAR binding depends less on the 2'-hydroxyl group of RNA than HuR binding. Finally we show that SAXS data, recorded for the first two domains of TIAR in complex with RNA, are more consistent with a flexible, elongated shape and not the compact shape that the first two domains of Hu proteins adopt upon binding to RNA. We thus propose that these triple-RRM proteins, which compete for the same binding sites in cells, interact with their targets in fundamentally different ways.

  13. User Instructions for the Systems Assessment Capability, Rev. 1, Computer Codes Volume 3: Utility Codes

    Energy Technology Data Exchange (ETDEWEB)

    Eslinger, Paul W.; Aaberg, Rosanne L.; Lopresti, Charles A.; Miley, Terri B.; Nichols, William E.; Strenge, Dennis L.

    2004-09-14

    This document contains detailed user instructions for a suite of utility codes developed for Rev. 1 of the Systems Assessment Capability. The suite of computer codes for Rev. 1 of Systems Assessment Capability performs many functions.

  14. Administrative Circulars No. 12 A (Rev. 2) - "Education fees” and No. 12 B (Rev. 2) - “Education fees and language courses”

    CERN Multimedia

    2013-01-01

    Administrative Circulars No. 12 A (Rev. 2) entitled “Education fees” and No. 12 B (Rev. 2) entitled “Education fees and language courses”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 27 June 2013 and entering into force on 1 August 2013, are available on the intranet site of the Human Resources Department (see here).   Administrative Circular No. 12 A (Rev. 2) is applicable to Staff Members (except former “Local Staff Members”) recruited before 1st January 2007. Administrative Circular No. 12 B (Rev. 2) is applicable to Staff Members recruited on or after 1st January 2007, to Fellows, to Scientific Associates, to Guest Professors and to former “Local Staff” recruited before 1st January 2007. They cancel and replace Administrative Circulars No. 12 A (Rev. 1/Corr.) entitled "Education fees” and No. 12 B (Rev. 1/Corr.) entitled “Edu...

  15. Sampling and estimation techniques for the implementation of new classification systems: the change-over from NACE Rev. 1.1 to NACE Rev. 2 in business surveys

    Directory of Open Access Journals (Sweden)

    Jan van den Brakel

    2010-09-01

    Full Text Available This paper describes some of the methodological problems encountered with the change-over from the NACE Rev. 1.1 to the NACE Rev. 2 in business statistics. Different sampling and estimation strategies are proposed to produce reliable figures for the domains under both classifications simultaneously. Furthermore several methods are described that can be used to reconstruct time series for the domains under the NACE Rev. 2.

  16. RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Feng; Zhang, Junsong; Zhang, Yijun; Geng, Guannan; Liang, Juanran; Li, Yingniang; Chen, Jingliang [Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Liu, Chao, E-mail: liuchao9@mail.sysu.edu.cn [Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Zhang, Hui [Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China); Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080 (China)

    2015-12-15

    Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various steps. However, the endogenous MOV10 was required in certain step(s) of HIV-1 replication. In this report, we found that MOV10 potently enhances the nuclear export of viral mRNAs and subsequently increases the expression of Gag protein and other late products through affecting the Rev/RRE axis. The co-immunoprecipitation analysis indicated that MOV10 interacts with Rev in an RNA-independent manner. The DEAG-box of MOV10 was required for the enhancement of Rev/RRE-dependent nuclear export and the DEAG-box mutant showed a dominant-negative activity. Our data propose that HIV-1 utilizes the anti-viral factor MOV10 to function as a co-factor of Rev and demonstrate the complicated effects of MOV10 on HIV-1 life cycle. - Highlights: • MOV10 can function as a co-factor of HIV-1 Rev. • MOV10 facilitates Rev/RRE-dependent transport of viral mRNAs. • MOV10 interacts with Rev in an RNA-independent manner. • The DEAG-box of MOV10 is required for the enhancement of Rev/RRE-dependent export.

  17. Chemistry Teacher Education Coalition: Extending the PhysTEC Model to Chemistry

    Science.gov (United States)

    Kirchhoff, Mary

    2012-02-01

    The American Association of Employment in Education reports that chemistry, like physics, faces ``some shortage'' of educators. Inspired by the success of the Physics Teacher Education Coalition (PhysTEC), the American Chemical Society (ACS) is developing the Chemistry Teacher Education Coalition (CTEC) to actively engage chemistry departments in the preparation of future chemistry teachers. Engaging chemistry departments in teacher preparation would increase the number and diversity of well-prepared high school chemistry teachers while catalyzing cultural change within chemistry departments. Many features of PhysTEC, such as a grant competition to create model teacher preparation programs and regular conferences, are directly applicable to chemistry. This presentation will provide an overview of ACS efforts to launch a successful CTEC initiative.

  18. Comment on Weakly dissipative dust-ion acoustic wave modulation (J. Plasma Phys. 82, 905820104, 2016)

    Science.gov (United States)

    Kourakis, I.; Elkamash, I. S.

    2016-10-01

    In a recent article (J. Plasma Phys., vol. 82, 2009, 905820104), weakly dissipative dust-ion acoustic wave modulation in dusty plasmas was considered. It is shown in this Comment that the analysis therein involved severe fallacies, and is in fact based on an erroneous plasma fluid model, which fails to satisfy an equilibrium condition, among other shortcomings. The subsequent analysis therefore is dubious and of limited scientific value.

  19. Design of octahedral spherical hohlraum for CH Rev5 ignition capsule

    Science.gov (United States)

    Cao, Hui; Chen, Yao-Hua; Zhai, Chuanlei; Zheng, Chunyang; Lan, Ke

    2017-08-01

    In this paper, we design an octahedral spherical Au hohlraum for CH Rev5 ignition capsule [S. W. Haan et al., Phys. Plasmas 18, 051001 (2011)] by using the initial design method and two-dimensional (2D) simulations, and we investigate its laser entrance hole (LEH) closure and laser-plasma instabilities (LPI) by using a spherical hohlraum with two different-size LEHs via 2D simulations. The designed spherical hohlraum with RH=5 RC, RL=1.2 mm , and RL*=2 RL requires an ignition laser pulse of 1.92 MJ in energy and 670 TW in peak power, where RH, RC, RL, and RL* are radii of the spherical hohlraum, capsule, LEH, and the cylindrical LEH outer ring, respectively. From 2D simulations, the closure and opening up of LEH are clearly obtained. The LEH closure and its rate are strongly connected to the radiation pulse, while the LEH opening-up and its rate are strongly connected to the laser pulse. The smallest radius of LEH during closure is 0.6 mm before opening up, which leaves enough room for arranging the laser beams with a radius of 0.5 mm in our design. By using a post-process code for LPI, a relatively high stimulated Brillouin scattering fraction and a very low stimulated Raman scattering fraction are predicted, which may be due to the neglection of three-dimensional density gradients of the ablative flow along the laser transportation in 2D simulations. This work provides the energy and power references for the future ignition laser facility which uses octahedral spherical hohlraums as ignition targets.

  20. Functional characterization of the HuR:CD83 mRNA interaction.

    Directory of Open Access Journals (Sweden)

    Dorothea Pieper

    Full Text Available Maturation of dendritic cells (DC is characterized by expression of CD83, a surface protein that appears to be necessary for the effective activation of naïve T-cells and T-helper cells by DC. Lately it was shown that CD83 expression is regulated on the posttranscriptional level by interaction of the shuttle protein HuR with a novel posttranscriptional regulatory RNA element (PRE, which is located in the coding region of the CD83 transcript. Interestingly, this interaction commits the CD83 mRNA to efficient nuclear export via the CRM1 pathway. To date, however, the structural basis of this interaction, which potentially involves three distinct RNA recognition motifs (RRM1-3 in HuR and a complex three-pronged RNA stem-loop element in CD83 mRNA, has not been investigated in detail. In the present work we analyzed this interaction in vitro and in vivo using various HuR- and CD83 mRNA mutants. We are able to demonstrate that both, RRM1 and RRM2 are crucial for binding, whereas RRM3 as well as the HuR hinge region contributed only marginally to this protein:RNA interaction. Furthermore, mutation of uridine rich patches within the PRE did not disturb HuR:CD83 mRNA complex formation while, in contrast, the deletion of specific PRE subfragments from the CD83 mRNA prevented HuR binding in vitro and in vivo. Interestingly, the observed inhibition of HuR binding to CD83 mRNA does not lead to a nuclear trapping of the transcript but rather redirected this transcript from the CRM1- towards the NXF1/TAP-specific nuclear export pathway. Thus, the presence of a functional PRE permits nucleocytoplasmic trafficking of the CD83 transcript via the CRM1 pathway.

  1. 12MW Horns Rev experiment[Wind farm

    Energy Technology Data Exchange (ETDEWEB)

    Hasager, C.B.; Pena, A; Mikkelsen, T.; Courtney, M.; Antoniou, I.; Gryning, S.-E.; Hansen, P. [Risoe National Lab., DTU, Wind Energy Dept. (Denmark); Soerensen, P.B. [DONG Energy (Denmark)

    2007-10-15

    The 12MW project with the full title '12 MW wind turbines: the scientific basis for their operation at 70 to 270 m height offshore' has the goal to experimentally investigate the wind and turbulence characteristics between 70 and 270 m above sea level and thereby establish the scientific basis relevant for the next generation of huge 12 MW wind turbines operating offshore. The report describes the experimental campaign at the Horns Rev offshore wind farm at which observations from Doppler Laser LIDAR and SODAR were collected from 3 May to 24 October 2006. The challenges for mounting and operating the instruments on the transformer platform at Horns Rev were overcome by a close collaboration between DONG energy and Risoe National Laboratory DTU. The site is presented. In particular, three tall offshore meteorological masts, up to 70 m tall, provided a useful source of meteorological data for comparison to the remotely sensed wind and turbulence observations. The comparison showed high correlation. The LIDAR and SODAR wind and turbulence observations were collected far beyond the height of the masts (up to 160 m above sea level) and the extended profiles were compared to the logarithmic wind profile. Further studies on this part of the work are on-going. Technical detail on LIDAR and SODAR are provided as well as theoretical work on turbulence and atmospheric boundary layer flow. Selected results from the experimental campaign are reported. (au)

  2. Comparative analysis of Rev function in human immunodeficiency virus types 1 and 2.

    OpenAIRE

    Garrett, E D; Cullen, B R

    1992-01-01

    The Rev proteins of the related but distinct human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) display incomplete functional reciprocity. One possible explanation for this observation is that HIV-2 Rev is unable to interact with the HIV-1 Rev-response element (RRE1). However, an analysis of the biological activity of chimeric proteins derived from HIV-1 and HIV-2 Rev reveals that this target specificity does not map to the Rev RNA binding domain but is instead primarily determined ...

  3. Analysis of the virulence-associated RevSR two-component signal transduction system of Clostridium perfringens.

    Science.gov (United States)

    Cheung, Jackie K; Wisniewski, Jessica A; Adams, Vicki M; Quinsey, Noelene S; Rood, Julian I

    2016-09-01

    Clostridium perfringens is a Gram-positive, anaerobic, spore-forming bacterium that causes human gas gangrene (clostridial myonecrosis) and food poisoning. Early studies showed that virulence was regulated by the VirSR two-component signal transduction system. However, our identification of the RevR orphan response regulator indicated that more than one system was involved in controlling virulence. To further characterize this virulence-associated regulator, gel mobility shift experiments, coupled with DNase I footprinting, were used to identify the RevR DNA binding sequence. Bioinformatics analysis suggested that an orphan sensor histidine kinase, CPE1757 (renamed RevS), was the cognate sensor of RevR. Interaction between RevS and RevR was demonstrated by use of a bacterial two-hybrid system and validated by protein-protein interaction studies using biolayer interferometry. To assess the involvement of RevS in virulence regulation, the revS gene was inactivated by Targetron insertion. When isogenic wild-type, revS and complemented revS strains were tested in a mouse myonecrosis model, the revS mutant was found to be attenuated in virulence, which was similar to the attenuation observed previously with the revR mutant. However, transcriptional analysis of selected RevR-regulated genes in the revS mutant revealed a different pattern of expression to a revR mutant, suggesting that the RevSR system is more complex than originally thought. Taken together, the results have led to the identification and characterization of the two essential parts of a new regulatory network that is involved in the regulation of virulence in C. perfringens.

  4. Hydroxyurea (HU) for prevention of recurrent stroke in sickle cell anemia (SCA).

    Science.gov (United States)

    Sumoza, Abraham; de Bisotti, Renate; Sumoza, David; Fairbanks, Virgil

    2002-11-01

    Cerebrovascular accident (CVA) is a major cause of morbidity and death in sickle cell anemia (SCA). Transfusion of packed erythrocytes is widely used to prevent this complication. However, chronic transfusion may lead to iron overload, alloimmunization, or infections. Cost and compliance may compromise transfusion therapy. A possible alternative, the prophylactic use of hydroxyurea (HU), has not been tried to determine whether it may prevent recurrent stroke. We used HU in five children with SCA who had suffered stroke, in three of them after a first episode and in the other two after a second CVA. Four had infarctive stroke and one a transient ischemic attack (TIA). Four patients took HU at a dose of 40 mg/kg/d, one patient at 30 mg/kg/d. None of the patients had recurrent stroke during 42-112 months of observation. None experienced pain crises. In all, HbF increased significantly and was maintained above 14.7% during treatment. The total Hb concentration increased 19.5 g/L (median) above the value before treatment. HU was well tolerated. None of the five children had leukopenia or thrombocytopenia during therapy. HU appears to prevent recurrence of stroke in SCA without risk of major toxicity.

  5. dNTP deficiency induced by HU via inhibiting ribonucleotide reductase affects neural tube development.

    Science.gov (United States)

    Guan, Zhen; Wang, Xiuwei; Dong, Yanting; Xu, Lin; Zhu, Zhiqiang; Wang, Jianhua; Zhang, Ting; Niu, Bo

    2015-02-03

    Exposure to environmental toxic chemicals in utero during the neural tube development period can cause developmental disorders. To evaluate the disruption of neural tube development programming, the murine neural tube defects (NTDs) model was induced by interrupting folate metabolism using methotrexate in our previous study. The present study aimed to examine the effects of dNTP deficiency induced by hydroxyurea (HU), a specific ribonucleotide reductase (RNR) inhibitor, during murine neural tube development. Pregnant C57BL/6J mice were intraperitoneally injected with various doses of HU on gestation day (GD) 7.5, and the embryos were checked on GD 11.5. RNR activity and deoxynucleoside triphosphate (dNTP) levels were measured in the optimal dose. Additionally, DNA damage was examined by comet analysis and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. Cellular behaviors in NTDs embryos were evaluated with phosphorylation of histone H3 (PH-3) and caspase-3 using immunohistochemistry and western blot analysis. The results showed that NTDs were observed mostly with HU treatment at an optimal dose of 225 mg/kg b/w. RNR activity was inhibited and dNTP levels were decreased in HU-treated embryos with NTDs. Additionally, increased DNA damage, decreased proliferation, and increased caspase-3 were significant in NTDs embryos compared to the controls. Results indicated that HU induced murine NTDs model by disturbing dNTP metabolism and further led to the abnormal cell balance between proliferation and apoptosis.

  6. Publication Of Administrative Circulars: No. 4 (Rev. 4) – Unemployment Insurance Scheme No. 30 (Rev. 2) – Financial Benefits on Taking Up Appointment and on Termination of Contract

    CERN Multimedia

    HR Department

    2008-01-01

    Administrative Circular No. 4 (Rev. 4) – Unemployment insurance scheme Administrative Circular No. 4 (Rev. 4) – "Unemployment insurance scheme", approved following discussion at the Standing Concertation Committee meetings of 28 August 2007 and 27 February 2008, is now available on the intranet site of the Human Resources Department. It cancels and replaces Administrative Circular No. 4 (Rev. 3) – "Unemployment insurance" of October 1993. Copies will shortly be available in Departmental secretariats. Human Resources Department Tel. 78003 Administrative Circular No. 30 (Rev. 2) – Financial benefits on taking up appointment and termination of contract Administrative Circular No. 30 (Rev. 2) – "Financial benefits on taking up appointment and termination of contract", approved following discussion at the Standing Concertation Committee meetings of 28 August 2007 and 27 February 2008, is now available on the intranet site of the Human Resources De...

  7. Publication Of Administrative Circulars: No. 4 (Rev. 4) – Unemployment Insurance Scheme No. 30 (Rev. 2) – Financial Benefits on Taking Up Appointment and on Termination of Contract

    CERN Multimedia

    HR Department

    2008-01-01

    Administrative Circular No. 4 (Rev. 4) – Unemployment insurance scheme Administrative Circular No. 4 (Rev. 4) – "Unemployment insurance scheme", approved following discussion in the Standing Concertation Committee meetings of 28 August 2007 and 27 February 2008, is now available on the intranet site of the Human Resources Department. It cancels and replaces Administrative Circular No. 4 (Rev. 3) – "Unemployment insurance" of October 1993. Copies will shortly be available in Departmental secretariats. Human Resources Department Tel. 78003 Administrative Circular No. 30 (Rev. 2) – Financial benefits on taking up appointment and termination of contract Administrative Circular No. 30 (Rev. 2) – "Financial benefits on taking up appointment and termination of contract", approved following discussion in the Standing Concertation Committee meetings of 28 August 2007 and 27 February 2008, is now available on the intranet site of the Human Resources De...

  8. Multiple Functions of the RNA-Binding Protein HuR in Cancer Progression, Treatment Responses and Prognosis

    Directory of Open Access Journals (Sweden)

    Baocheng Wang

    2013-05-01

    Full Text Available The human embryonic lethal abnormal vision-like protein, HuR, is a member of the Hu family of RNA-binding proteins. Over the past decade, this ubiquitously expressed protein has been extensively investigated in cancer research because it is involved in the regulation of mRNA stability and translation in many cell types. HuR activity and function is associated with its subcellular distribution, transcriptional regulation, translational and post-translational modifications. HuR regulation of target mRNAs is based on the interaction between the three specific domains of HuR protein and one or several U- or AU-rich elements (AREs in the untranslated region of target mRNAs. A number of cancer-related transcripts containing AREs, including mRNAs for proto-oncogenes, cytokines, growth factors, and invasion factors, have been characterized as HuR targets. It has been proposed that HuR has a central tumorigenic activity by enabling multiple cancer phenotypes. In this review, we comprehensively survey the existing evidence with regard to the diverse functions of HuR in caner development and progression. The current data also suggest that HuR might be a novel and promising therapeutic target and a marker for treatment response and prognostic evaluation.

  9. Temporally defined neocortical translation and polysome assembly are determined by the RNA-binding protein Hu antigen R.

    Science.gov (United States)

    Kraushar, Matthew L; Thompson, Kevin; Wijeratne, H R Sagara; Viljetic, Barbara; Sakers, Kristina; Marson, Justin W; Kontoyiannis, Dimitris L; Buyske, Steven; Hart, Ronald P; Rasin, Mladen-Roko

    2014-09-09

    Precise spatiotemporal control of mRNA translation machinery is essential to the development of highly complex systems like the neocortex. However, spatiotemporal regulation of translation machinery in the developing neocortex remains poorly understood. Here, we show that an RNA-binding protein, Hu antigen R (HuR), regulates both neocorticogenesis and specificity of neocortical translation machinery in a developmental stage-dependent manner in mice. Neocortical absence of HuR alters the phosphorylation states of initiation and elongation factors in the core translation machinery. In addition, HuR regulates the temporally specific positioning of functionally related mRNAs into the active translation sites, the polysomes. HuR also determines the specificity of neocortical polysomes by defining their combinatorial composition of ribosomal proteins and initiation and elongation factors. For some HuR-dependent proteins, the association with polysomes likewise depends on the eukaryotic initiation factor 2 alpha kinase 4, which associates with HuR in prenatal developing neocortices. Finally, we found that deletion of HuR before embryonic day 10 disrupts both neocortical lamination and formation of the main neocortical commissure, the corpus callosum. Our study identifies a crucial role for HuR in neocortical development as a translational gatekeeper for functionally related mRNA subgroups and polysomal protein specificity.

  10. Coordination of genomic structure and transcription by the main bacterial nucleoid-associated protein HU.

    Science.gov (United States)

    Berger, Michael; Farcas, Anca; Geertz, Marcel; Zhelyazkova, Petya; Brix, Klaudia; Travers, Andrew; Muskhelishvili, Georgi

    2010-01-01

    The histone-like protein HU is a highly abundant DNA architectural protein that is involved in compacting the DNA of the bacterial nucleoid and in regulating the main DNA transactions, including gene transcription. However, the coordination of the genomic structure and function by HU is poorly understood. Here, we address this question by comparing transcript patterns and spatial distributions of RNA polymerase in Escherichia coli wild-type and hupA/B mutant cells. We demonstrate that, in mutant cells, upregulated genes are preferentially clustered in a large chromosomal domain comprising the ribosomal RNA operons organized on both sides of OriC. Furthermore, we show that, in parallel to this transcription asymmetry, mutant cells are also impaired in forming the transcription foci-spatially confined aggregations of RNA polymerase molecules transcribing strong ribosomal RNA operons. Our data thus implicate HU in coordinating the global genomic structure and function by regulating the spatial distribution of RNA polymerase in the nucleoid.

  11. Geology of outer Horns Rev, Danish North Sea

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, Joern B.; Gravesen, P.; Lomholt, S. (Geological Survey of Denmark and Greenland, Copenhagen (Denmark))

    2008-07-15

    In 2006, Dong Energy initiated the development of the Horns Rev II offshore wind farm in the North Sea. In order to evaluate and map the characteristics of the surface features of the sea bed and to characterise the subsurface in the wind farm area, the Geological Survey of Denmark and Greenland (GEUS) conducted a geophysical survey of the area. The survey utilised a variety of instruments: sparker, side-scan sonar, marine caesium magnetometer and a multibeam echo-sounder. In addition, information on the subsurface sediments was obtained by cone penetration tests (CPT) and by drilling to 30-50 m below the sea bottom. Geological correlation of the CPT results with the other survey results was extremely complicated but was required in order to understand the architecture of the ice marginal glaciotectonic complex. Information on the geology is crucial for evaluation of the geotechnical problems of the region. (au)

  12. Ensemble-based Probabilistic Forecasting at Horns Rev

    DEFF Research Database (Denmark)

    Pinson, Pierre; Madsen, Henrik

    2009-01-01

    of probabilistic forecasts, the resolution of which may be maximized by using meteorological ensemble predictions as input. The paper concentrates on the test case of the Horns Rev wind form over a period of approximately 1 year, in order to describe, apply and discuss a complete ensemble-based probabilistic...... the benefit of yielding predictive distributions that are of increased reliability (in a probabilistic sense) in comparison with the raw ensemble forecasts, at the some time taking advantage of their high resolution. Copyright (C) 2008 John Wiley & Sons, Ltd....... are then converted into predictive distributions with an original adaptive kernel dressing method. The shape of the kernels is driven by a mean-variance model, the parameters of which ore recursively estimated in order to maximize the overall skill of obtained predictive distributions. Such a methodology has...

  13. Human REV3 DNA Polymerase Zeta Localizes to Mitochondria and Protects the Mitochondrial Genome.

    Science.gov (United States)

    Singh, Bhupendra; Li, Xiurong; Owens, Kjerstin M; Vanniarajan, Ayyasamy; Liang, Ping; Singh, Keshav K

    2015-01-01

    To date, mitochondrial DNA polymerase γ (POLG) is the only polymerase known to be present in mammalian mitochondria. A dogma in the mitochondria field is that there is no other polymerase present in the mitochondria of mammalian cells. Here we demonstrate localization of REV3 DNA polymerase in the mammalian mitochondria. We demonstrate localization of REV3 in the mitochondria of mammalian tissue as well as cell lines. REV3 associates with POLG and mitochondrial DNA and protects the mitochondrial genome from DNA damage. Inactivation of Rev3 leads to reduced mitochondrial membrane potential, reduced OXPHOS activity, and increased glucose consumption. Conversely, inhibition of the OXPHOS increases expression of Rev3. Rev3 expression is increased in human primary breast tumors and breast cancer cell lines. Inactivation of Rev3 decreases cell migration and invasion, and localization of Rev3 in mitochondria increases survival and the invasive potential of cancer cells. Taken together, we demonstrate that REV3 functions in mammalian mitochondria and that mitochondrial REV3 is associated with the tumorigenic potential of cells.

  14. RNA-guided assembly of Rev-RRE nuclear export complexes.

    Science.gov (United States)

    Bai, Yun; Tambe, Akshay; Zhou, Kaihong; Doudna, Jennifer A

    2014-08-27

    HIV replication requires nuclear export of unspliced and singly spliced viral transcripts. Although a unique RNA structure has been proposed for the Rev-response element (RRE) responsible for viral mRNA export, how it recruits multiple HIV Rev proteins to form an export complex has been unclear. We show here that initial binding of Rev to the RRE triggers RNA tertiary structural changes, enabling further Rev binding and the rapid formation of a viral export complex. Analysis of the Rev-RRE assembly pathway using SHAPE-Seq and small-angle X-ray scattering (SAXS) reveals two major steps of Rev-RRE complex formation, beginning with rapid Rev binding to a pre-organized region presenting multiple Rev binding sites. This step induces long-range remodeling of the RNA to expose a cryptic Rev binding site, enabling rapid assembly of additional Rev proteins into the RNA export complex. This kinetic pathway may help maintain the balance between viral replication and maturation.DOI: http://dx.doi.org/10.7554/eLife.03656.001.

  15. REV3L modulates cisplatin sensitivity of non-small cell lung cancer H1299 cells.

    Science.gov (United States)

    Wang, Wenjie; Sheng, Wenjiong; Yu, Chenxiao; Cao, Jianping; Zhou, Jundong; Wu, Jinchang; Zhang, Huojun; Zhang, Shuyu

    2015-09-01

    Lung cancer remains the leading cause of cancer-related mortality worldwide and non-small cell lung cancer (NSCLC) accounts for approximately 80-85% of all cases of lung cancer. Cisplatin plays a significant role in the management of human lung cancer. Translesion DNA synthesis (TLS) is involved in DNA damage repair. DNA polymerase ζ (Pol ζ) is able to mediate the DNA replication bypass of DNA damage, which is suggested to be involved in chemoresistance. REV3L is the catalytic subunit of Pol ζ. Due to its critical role in translesion DNA synthesis, whether REV3L modulates cisplatin response in NSCLC cells remains unknown. In this study, REV3L overexpression and silencing H1299 cell lines were established. The reports showed that cisplatin induced the expression of REV3L by recruiting Sp1 to its promoter. Similar results were obtained when the ability of the cells to express luciferase from a platinated plasmid was measured. Co-transfection of the reporter with the REV3L overexpression vector or REV3L plus REV7L significantly enhanced the reporter activity. Nuclear condensation and fragmentation of shRNA-REV3L H1299 cells were more pronounced than shRNA-NC H1299 cells after cisplatin exposure, indicating that REV3L overexpression abolished cisplatin-induced DNA damage. Moreover, a forced expression of REV3L conferred the resistance of H1299 cells to cisplatin, whereas the knockdown of REV3L sensitized cisplatin efficacy in H1299 cells. Taken together, we demonstrated that inhibition of REV3L sensitized lung cancer H1299 cells to cisplatin treatment. Thus, REV3L may be a novel target for the chemotherapy of NSCLC.

  16. Ship Targets Discrimination Algorithm in SAR Images Based on Hu Moment Feature and Texture Feature

    Directory of Open Access Journals (Sweden)

    Liu Lei

    2016-01-01

    Full Text Available To discriminate the ship targets in SAR images, this paper proposed the method based on combination of Hu moment feature and texture feature. Firstly,7 Hu moment features should be extracted, while gray level co-occurrence matrix is then used to extract the features of mean, variance, uniformity, energy, entropy, inertia moment, correlation and differences. Finally the k-neighbour classifier was used to analysis the 15 dimensional feature vectors. The experimental results show that the method of this paper has a good effect.

  17. Neuroprotective effects of the cannabinoid agonist HU210 on retinal degeneration

    OpenAIRE

    Lax Zapata, Pedro; Esquiva Sobrino, Gema; Altavilla, Cesare; Cuenca Navarro, Nicolás

    2014-01-01

    Cannabinoids have been demonstrated to exert neuroprotective effects on different types of neuronal insults. Here we have addressed the therapeutic potential of the synthetic cannabinoid HU210 on photoreceptor degeneration, synaptic connectivity and functional activity of the retina in the transgenic P23H rat, an animal model for autosomal dominant retinitis pigmentosa (RP). In P23H rats administered with HU210 (100 μg/kg, i.p.) from P24 to P90, ERG recordings showed an amelioration of vision...

  18. The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA.

    Science.gov (United States)

    López de Silanes, Isabel; Gorospe, Myriam; Taniguchi, Hiroaki; Abdelmohsen, Kotb; Srikantan, Subramanya; Alaminos, Miguel; Berdasco, María; Urdinguio, Rocío G; Fraga, Mario F; Jacinto, Filipe V; Esteller, Manel

    2009-05-01

    The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression levels increase during tumorigenesis. Here, we present evidence that the expression of DNMT3b is post-transcriptionally regulated by HuR, an RNA-binding protein that stabilizes and/or modulates the translation of target mRNAs. The presence of a putative HuR-recognition motif in the DNMT3b 3'UTR prompted studies to investigate if this transcript associated with HuR. The interaction between HuR and DNMT3b mRNA was studied by immunoprecipitation of endogenous HuR ribonucleoprotein complexes followed by RT-qPCR detection of DNMT3b mRNA, and by in vitro pulldown of biotinylated DNMT3b RNAs followed by western blotting detection of HuR. These studies revealed that binding of HuR stabilized the DNMT3b mRNA and increased DNMT3b expression. Unexpectedly, cisplatin treatment triggered the dissociation of the [HuR-DNMT3b mRNA] complex, in turn promoting DNMT3b mRNA decay, decreasing DNMT3b abundance, and lowering the methylation of repeated sequences and global DNA methylation. In summary, our data identify DNMT3b mRNA as a novel HuR target, present evidence that HuR affects DNMT3b expression levels post-transcriptionally, and reveal the functional consequences of the HuR-regulated DNMT3b upon DNA methylation patterns.

  19. Hu Shi and the Continuation of the Revolution of1911——Discussing Intellectuals and Social Revolution%Hu Shi and the Continuation of the Revolution of 1911——Discussing Intellectuals and Social Revolution

    Institute of Scientific and Technical Information of China (English)

    Zhu Cheng

    2011-01-01

    Hu Shi is regarded as one of the most important intellectuals after the Revolution of 1911,and he has a far- reaching influence in 20th century.In the perspective of Hu Shi,the contribution and continuation of the Revolution of 1911 has some inspiration for us to analyze the relationship between Chinese intellectuals and social revolution.

  20. Comment on the paper Photoionization of endohedral atoms using R-matrix methods: application to Xe@C60 by T. W. Gorczyca, M. F. Hasoglu and S. T. Manson, Phys. Rev. A86, 033204 (2012) [1

    CERN Document Server

    Amusia, M Ya

    2013-01-01

    We demonstrate serious misrepresentation of existing experimental data and important omissions in the List of references in the paper [1]. We demonstrate that what is called in [1] total photoionization cross-section is in fact a partial photoionization cross-section. We demonstrate that long before [1] and presented there experimental data for photoionization of Xe@C60+ were obtained, a theoretical prediction for Xe@C60 have been published that was in a reasonable agreement with experiment. These data are even not mentioned in [1].

  1. Comment on the numerical measurements of the magnetohydrodynamic turbulence spectrum by A. Beresnyak (Phys. Rev. Lett. 106 (2011) 075001; MNRAS 422 (2012) 3495; ApJ 784 (2014) L20)

    CERN Document Server

    Perez, J C; Boldyrev, S; Cattaneo, F

    2014-01-01

    The inertial-interval energy spectrum of strong magnetohydrodynamic (MHD) turbulence with a uniform background magnetic field was observed numerically to be close to $k^{-3/2}$ by a number of independent groups. A dissenting opinion has been voiced by Beresnyak, A. 2011, PRL, 106, 075001-. 2012, MNRAS, 422, 3495-. 2014, ApJ, 784, L20 that the spectral scaling is close to $k^{-5/3}$. The conclusions of these papers are however incorrect as they are based on numerical simulations that are drastically unresolved, so that the discrete numerical scheme does not approximate the physical solution at the scales where the measurements are performed. These results have been rebutted in our more detailed papers Perez, J. C., Mason, J., Boldyrev, S., & Cattaneo, F. 2012, PRX, 2, 041005-. 2014, ApJL, 793, L13; here, by popular demand, we present a brief and simple explanation of our major criticism of Beresnyak's work.

  2. Publication of administrative circular No. 26 (Rev. 8) – Recognition of merit

    CERN Multimedia

    HR Department

    2008-01-01

    Administrative Circular No. 26 (Rev. 8) – Recognition of merit Administrative Circular No. 26 (Rev. 8) – "Recognition of merit", approved by the Standing Concertation Committee at its meeting on 3 September 2008, is now available on the intranet site of the Human Resources Department. It cancels and replaces Administrative Circular No. 26 (Rev. 7) – "Recognition of merit of staff members" of May 2007. Paper copies will shortly be available in Departmental secretariats. Human Resources Department Tel. 78003

  3. Publication of administrative circular No. 26 (Rev. 8) – Recognition of merit

    CERN Multimedia

    HR Department

    2008-01-01

    Administrative Circular No. 26 (Rev. 8) – Recognition of merit Administrative Circular No. 26 (Rev. 8) – "Recognition of merit", approved by the Standing Concertation Committee meeting on 3 September 2008, is now available on the intranet site of the Human Resources Department. It cancels and replaces Administrative Circular No. 26 (Rev. 7) – "Recognition of merit of staff members" of May 2007. Paper copies will shortly be available in Departmental secretariats. Human Resources Department Tel. 78003

  4. Suured fondid on varunud börsile mineva Teede REV-2 aktsiaid / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Seoses Teede REV-2 aktsionäride kavaga aktsiate noteerimiseks Tallinna börsil 2005. aasta alguses, on Hansa Balti Kasvufond, Sampo Uus Euroopa Fond ja Ühispanga Kasvufond ostnud hallatavatesse investeerimisportfellidesse ehitusettevõtte aktsiaid. Diagrammid: Suure osa Teede REV-2 aktsiate omanikest tegevjuhid; Teede-Rev 2 ootab tänavu käibe kasvu. Kommenteerib Aivaras Abromavicius

  5. The HU Regulon Is Composed of Genes Responding to Anaerobiosis, Acid Stress, High Osmolarity and SOS Induction

    Science.gov (United States)

    Oberto, Jacques; Nabti, Sabrina; Jooste, Valérie; Mignot, Hervé; Rouviere-Yaniv, Josette

    2009-01-01

    Background The Escherichia coli heterodimeric HU protein is a small DNA-bending protein associated with the bacterial nucleoid. It can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Cells lacking HU grow very poorly and display many phenotypes. Methodology/Principal Findings We analyzed the transcription profile of every Escherichia coli gene in the absence of one or both HU subunits. This genome-wide in silico transcriptomic approach, performed in parallel with in vivo genetic experimentation, defined the HU regulon. This large regulon, which comprises 8% of the genome, is composed of four biologically relevant gene classes whose regulation responds to anaerobiosis, acid stress, high osmolarity, and SOS induction. Conclusions/Significance The regulation a large number of genes encoding enzymes involved in energy metabolism and catabolism pathways by HU explains the highly pleiotropic phenotype of HU-deficient cells. The uniform chromosomal distribution of the many operons regulated by HU strongly suggests that the transcriptional and nucleoid architectural functions of HU constitute two aspects of a unique protein-DNA interaction mechanism. PMID:19194530

  6. The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction.

    Directory of Open Access Journals (Sweden)

    Jacques Oberto

    Full Text Available BACKGROUND: The Escherichia coli heterodimeric HU protein is a small DNA-bending protein associated with the bacterial nucleoid. It can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Cells lacking HU grow very poorly and display many phenotypes. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed the transcription profile of every Escherichia coli gene in the absence of one or both HU subunits. This genome-wide in silico transcriptomic approach, performed in parallel with in vivo genetic experimentation, defined the HU regulon. This large regulon, which comprises 8% of the genome, is composed of four biologically relevant gene classes whose regulation responds to anaerobiosis, acid stress, high osmolarity, and SOS induction. CONCLUSIONS/SIGNIFICANCE: The regulation a large number of genes encoding enzymes involved in energy metabolism and catabolism pathways by HU explains the highly pleiotropic phenotype of HU-deficient cells. The uniform chromosomal distribution of the many operons regulated by HU strongly suggests that the transcriptional and nucleoid architectural functions of HU constitute two aspects of a unique protein-DNA interaction mechanism.

  7. Immunostimulatory effects of natural human interferon-alpha (huIFN-alpha) on carps Cyprinus carpio L.

    Science.gov (United States)

    Watanuki, Hironobu; Chakraborty, Gunimala; Korenaga, Hiroki; Kono, Tomoya; Shivappa, R B; Sakai, Masahiro

    2009-10-15

    Human interferon-alpha (huIFN-alpha) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. However, the immunostimulatory effects of huIFN-alpha in fish remain to be investigated. In the current study, the immune responses of the carp species Cyprinus carpio L. to treatment with huIFN-alpha were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes including interleukin-1beta, tumor necrosis factor-alpha and interleukin 10. Low doses of huIFN-alpha were administered orally once a day for 3 days, and sampling was carried out at 1, 3 and 5 days post-treatment. Our results indicate that a low dose of huIFN-alpha significantly increased phagocytic activity and superoxide anion production in the carp kidney. The huIFN-alpha-treated fish also displayed a significant upregulation in cytokine gene expression. The current study demonstrates the stimulatory effects of huIFN-alpha on the carp immune system and highlights the immunomodulatory role of huIFN-alpha in fish.

  8. DDX5 facilitates HIV-1 replication as a cellular co-factor of Rev.

    Directory of Open Access Journals (Sweden)

    Xiuxia Zhou

    Full Text Available HIV-1 Rev plays an important role in the late phase of HIV-1 replication, which facilitates export of unspliced viral mRNAs from the nucleus to cytoplasm in infected cells. Recent studies have shown that DDX1 and DDX3 are co-factors of Rev for the export of HIV-1 transcripts. In this report, we have demonstrated that DDX5 (p68, which is a multifunctional DEAD-box RNA helicase, functions as a new cellular co-factor of HIV-1 Rev. We found that DDX5 affects Rev function through the Rev-RRE axis and subsequently enhances HIV-1 replication. Confocal microscopy and co-immunoprecipitation analysis indicated that DDX5 binds to Rev and this interaction is largely dependent on RNA. If the DEAD-box motif of DDX5 is mutated, DDX5 loses almost all of its ability to bind to Rev, indicating that the DEAD-box motif of DDX5 is required for the interaction between DDX5 and Rev. Our data indicate that interference of DDX5-Rev interaction could reduce HIV-1 replication and potentially provide a new molecular target for anti-HIV-1 therapeutics.

  9. Identification of the first small-molecule inhibitor of the REV7 DNA repair protein interaction.

    Science.gov (United States)

    Actis, Marcelo L; Ambaye, Nigus D; Evison, Benjamin J; Shao, Youming; Vanarotti, Murugendra; Inoue, Akira; McDonald, Ezelle T; Kikuchi, Sotaro; Heath, Richard; Hara, Kodai; Hashimoto, Hiroshi; Fujii, Naoaki

    2016-09-15

    DNA interstrand crosslink (ICL) repair (ICLR) has been implicated in the resistance of cancer cells to ICL-inducing chemotherapeutic agents. Despite the clinical significance of ICL-inducing chemotherapy, few studies have focused on developing small-molecule inhibitors for ICLR. The mammalian DNA polymerase ζ, which comprises the catalytic subunit REV3L and the non-catalytic subunit REV7, is essential for ICLR. To identify small-molecule compounds that are mechanistically capable of inhibiting ICLR by targeting REV7, high-throughput screening and structure-activity relationship (SAR) analysis were performed. Compound 1 was identified as an inhibitor of the interaction of REV7 with the REV7-binding sequence of REV3L. Compound 7 (an optimized analog of compound 1) bound directly to REV7 in nuclear magnetic resonance analyses, and inhibited the reactivation of a reporter plasmid containing an ICL in between the promoter and reporter regions. The normalized clonogenic survival of HeLa cells treated with cisplatin and compound 7 was lower than that for cells treated with cisplatin only. These findings indicate that a small-molecule inhibitor of the REV7/REV3L interaction can chemosensitize cells by inhibiting ICLR.

  10. First International Workshop on Human Factors in Modeling (HuFaMo 2015)

    DEFF Research Database (Denmark)

    Störrle, Harald; Chaudron, Michel R. V.; Amaral, Vasco

    2015-01-01

    and systematic approaches to assert usability in modeling and confirm the claims of productivity. This workshop creates a space for discussion being a get together of both MDE, Usability, Human Interfaces and the Experimental Software engineering community. HuFaMo expressly focuses on human factors, in order...

  11. Rare Association of Anti-Hu Antibody Positive Paraneoplastic Neurological Syndrome and Transitional Cell Bladder Carcinoma

    Directory of Open Access Journals (Sweden)

    S. Lukacs

    2012-01-01

    Full Text Available Introduction. Paraneoplastic encephalomyelitis (PEM and subacute sensory neuronopathy (SSN are remote effects of cancer, usually associated with small-cell lung carcinoma and positive anti-Hu antibody. We describe the rare association of bladder transitional cell carcinoma (TCC with anti-Hu antibody positivity resulting in this paraneoplastic neurological syndrome. Patient. A 76-year-old female presented with bilateral muscle weakness and paraesthesia of the upper and lower limbs in a length-dependent “glove and stocking” distribution. Central nervous system symptoms included cognitive problems, personality change, and truncal ataxia. Case notes and the literature were reviewed. Result. Autoantibody screening was positive for anti-Hu antibody (recently renamed antineuronal nuclear antibody 1, ANNA-1. The diagnosis of PEM and SSN was supported by MRI and lumbar puncture results. A superficial bladder TCC was demonstrated on CT and subsequently confirmed on histology. No other primary neoplasm was found on full-body imaging. The neurological symptoms were considered to be an antibody-mediated paraneoplastic neurological syndrome and improved after resection of the tumour. Discussion. The association of anti-Hu positive paraneoplastic neurological syndrome and TCC has not been described in the literature previously. We emphasize the need for detailed clinical examination and the importance of a multidisciplinary thought process and encourage further awareness of this rare association.

  12. Comment on ``Minimum free-energy pathway of nucleation'' [J. Chem. Phys. 135, 134508 (2011)

    Science.gov (United States)

    Schmelzer, Jürn W. P.; Abyzov, Alexander S.

    2012-03-01

    In their recent paper [J. Chem. Phys. 135, 134508 (2011)], Philippe and Blavette, 10.1063/1.3644935 claimed to have found for the first time—based on minimum free energy considerations—that the nucleation pathway in solid solutions involves a universal two-step behavior: nuclei sharply enrich up to near equilibrium values of the concentration and then grow. This claim of priority is not correct. The same scenario was proposed by us long ago [J. W. P. Schmelzer, A. R. Gokhman, and V. M. Fokin, J. Colloid Interface Sci. 272, 109 (2004)], 10.1016/j.jcis.2003.08.038 and then applied and further developed. In particular, it was shown by us that only the account of both thermodynamic and kinetic factors allows one to determine correctly the most probable path of evolution in nucleation-growth processes. The two-step scenario is retained, however, also in these generalizations of our original approach.

  13. Comment on "Deterministic six states protocol for quantum communication" [Phys. Lett. A 358 (2006) 85

    CERN Document Server

    El-Orany, Faisal A A

    2011-01-01

    In [J.S. Shaari, M. Lucamarini, M.R.B. Wahiddin, Phys. Lett. A 358 (2006) 85-90] the deterministic six states protocol (6DP) for quantum communication has been developed. This protocol is based on three mutually unbiased bases and four encoding operators. Information is transmitted between the users via two qubits from different bases. Three attacks have been studied; namely intercept-resend attack (IRA), double-CNOT attack (2CNOTA) and quantum man-in-the-middle attack. In this Letter, we show that the IRA and 2CNOTA are not properly addressed. For instance, we show that the probability of detecting Eve in the control mode of the IRA is 70% instead of 50% in the previous study. Moreover, in the 2CNOTA, Eve can only obtain 50% of the data not all of it as argued earlier.

  14. HuB localizes to polysomes and alters C/EBP-beta expression in 3T3-L1 adipocytes.

    Science.gov (United States)

    Gantt, Kira R; Jain, Renu G; Dudek, Ronald W; Pekala, Phillip H

    2004-01-16

    The RNA binding protein HuB was ectopically expressed in 3T3-L1 preadipocytes and localized primarily to the nucleus, as the cells differentiated HuB redistributed to the cytosol and on analysis localized to the dense polysomes. Electron micrographs confirm association of HuB with the ribosomes in the adipocytes consistent with a proposed role in control of translation and mRNA stability. Examination of the expression of C/EBP-beta in the cells expressing HuB relative to the parental 3T3-L1 adipocytes demonstrated an alteration in the LAP to LIP ratio. The data support a role for endogenous Hu proteins in the differentiation process, potentially affecting the rate of differentiation by controlling the concentration of the dominant negative transcription inhibitor, LIP.

  15. Redox behaviors of the neurotoxic portion in human prion protein, HuPrP(106-126)

    Science.gov (United States)

    Yamamoto, Norifumi; Kuwata, Kazuo

    2010-09-01

    A peptide fragment of human prion protein, HuPrP(106-126), has been reported to mimic the pathological features underlying prion diseases. Although the actual neurotoxic mechanism of HuPrP(106-126) has not been elucidated, several hypotheses has been proposed based on the role for copper. In this study, to understand the toxic function of HuPrP(106-126) from a viewpoint of electrochemical competence, we investigated redox properties of copper ion complexes with four different binding motifs of a model of HuPrP(106-126) based on density functional theory calculations. We found that the HuPrP(106-126)-derived models exhibited diverse redox activities that depended on copper-binding conformations.

  16. Epistatic participation of REV1 and REV3 in the formation of UV-induced frameshift mutations in cell cycle-arrested yeast cells

    Energy Technology Data Exchange (ETDEWEB)

    Heidenreich, Erich [Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria)]. E-mail: erich.heidenreich@meduniwien.ac.at; Eisler, Herfried [Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria); Steinboeck, Ferdinand [Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria)

    2006-01-29

    Mutations arising in times of cell cycle arrest may provide a selective advantage for unicellular organisms adapting to environmental changes. For multicellular organisms, however, they may pose a serious threat, in that such mutations in somatic cells contribute to carcinogenesis and ageing. The budding yeast Saccharomyces cerevisiae presents a convenient model system for studying the incidence and the mechanisms of stationary-phase mutation in a eukaryotic organism. Having studied the emergence of frameshift mutants after several days of starvation-induced cell cycle arrest, we previously reported that all (potentially error-prone) translesion synthesis (TLS) enzymes identified in S. cerevisiae did not contribute to the basal level of spontaneous stationary-phase mutations. However, we observed that an increased frequency of stationary-phase frameshift mutations, brought about by a defective nucleotide excision repair (NER) pathway or by UV irradiation, was dependent on Rev3p, the catalytic subunit of the TLS polymerase zeta (Pol {zeta}). Employing the same two conditions, we now examined the effect of deletions of the genes coding for polymerase eta (Pol {eta}) (RAD30) and Rev1p (REV1). In a NER-deficient strain background, the increased incidence of stationary-phase mutations was only moderately influenced by a lack of Pol {eta} but completely reduced to wild type level by a knockout of the REV1 gene. UV-induced stationary-phase mutations were abundant in wild type and rad30{delta} strains, but substantially reduced in a rev1{delta} as well as a rev3{delta} strain. The similarity of the rev1{delta} and the rev3{delta} phenotype and an epistatic relationship evident from experiments with a double-deficient strain suggests a participation of Rev1p and Rev3p in the same mutagenic pathway. Based on these results, we propose that the response of cell cycle-arrested cells to an excess of exo- or endogenously induced DNA damage includes a novel replication

  17. Comment on “Effects of damping solitary wave in a viscosity bounded plasma” [Phys. Plasmas 21, 022118 (2014)

    Energy Technology Data Exchange (ETDEWEB)

    Ghosh, Uday Narayan, E-mail: unghosh1@rediffmail.com; Chatterjee, Prasanta; Roychoudhury, Rajkumar [Department of Mathematics, Siksha Bhavana, Visva Bharati, Santiniketan 731235 (India)

    2015-07-15

    Recently Gun Li et al. discussed “Effects of damping solitary wave in a viscosity bounded plasma” [Phys. Plasmas 21, 022118 (2014)]. The paper contains some serious errors which have been pointed out in this Comment.

  18. Wind Farm Wake: The Horns Rev Photo Case

    Directory of Open Access Journals (Sweden)

    Pierre-Elouan Réthoré

    2013-02-01

    Full Text Available The aim of the paper is to examine the nowadays well-known wind farm wake photographs taken on 12 February 2008 at the offshore Horns Rev 1 wind farm. The meteorological conditions are described from observations from several satellite sensors quantifying clouds, surface wind vectors and sea surface temperature as well as ground-based information at and near the wind farm, including Supervisory Control and Data Acquisition (SCADA data. The SCADA data reveal that the case of fog formation occurred 12 February 2008 on the 10:10 UTC. The fog formation is due to very special atmospheric conditions where a layer of cold humid air above a warmer sea surface re-condensates to fog in the wake of the turbines. The process is fed by warm humid air up-drafted from below in the counter-rotating swirl generated by the clock-wise rotating rotors. The condensation appears to take place primarily in the wake regions with relatively high axial velocities and high turbulent kinetic energy. The wind speed is near cut-in and most turbines produce very little power. The rotational pattern of spiraling bands produces the large-scale structure of the wake fog.

  19. Drug Transporter Expression and Activity in Human Hepatoma HuH-7 Cells

    Directory of Open Access Journals (Sweden)

    Elodie Jouan

    2016-12-01

    Full Text Available Human hepatoma cells may represent a valuable alternative to the use of human hepatocytes for studying hepatic drug transporters, which is now a regulatory issue during drug development. In the present work, we have characterized hepatic drug transporter expression, activity and regulation in human hepatoma HuH-7 cells, in order to determine the potential relevance of these cells for drug transport assays. HuH-7 cells displayed notable multidrug resistance-associated protein (MRP activity, presumed to reflect expression of various hepatic MRPs, including MRP2. By contrast, they failed to display functional activities of the uptake transporters sodium taurocholate co-transporting polypeptide (NTCP, organic anion-transporting polypeptides (OATPs and organic cation transporter 1 (OCT1, and of the canalicular transporters P-glycoprotein and breast cancer resistance protein (BCRP. Concomitantly, mRNA expressions of various sinusoidal and canalicular hepatic drug transporters were not detected (NTCP, OATP1B1, organic anion transporter 2 (OAT2, OCT1 and bile salt export pump or were found to be lower (OATP1B3, OATP2B1, multidrug and toxin extrusion protein 1, BCRP and MRP3 in hepatoma HuH-7 cells than those found in human hepatocytes, whereas other transporters such as OAT7, MRP4 and MRP5 were up-regulated. HuH-7 cells additionally exhibited farnesoid X receptor (FXR- and nuclear factor erythroid 2-related factor 2 (Nrf2-related up-regulation of some transporters. Such data indicate that HuH-7 cells, although expressing rather poorly some main hepatic drug transporters, may be useful for investigating interactions of drugs with MRPs, notably MRP2, and for studying FXR- or Nrf2-mediated gene regulation.

  20. Discriminating between HuR and TTP binding sites using the k-spectrum kernel method

    Science.gov (United States)

    Goldberg, Debra S.; Dowell, Robin

    2017-01-01

    Background The RNA binding proteins (RBPs) human antigen R (HuR) and Tristetraprolin (TTP) are known to exhibit competitive binding but have opposing effects on the bound messenger RNA (mRNA). How cells discriminate between the two proteins is an interesting problem. Machine learning approaches, such as support vector machines (SVMs), may be useful in the identification of discriminative features. However, this method has yet to be applied to studies of RNA binding protein motifs. Results Applying the k-spectrum kernel to a support vector machine (SVM), we first verified the published binding sites of both HuR and TTP. Additional feature engineering highlighted the U-rich binding preference of HuR and AU-rich binding preference for TTP. Domain adaptation along with multi-task learning was used to predict the common binding sites. Conclusion The distinction between HuR and TTP binding appears to be subtle content features. HuR prefers strongly U-rich sequences whereas TTP prefers AU-rich as with increasing A content, the sequences are more likely to be bound only by TTP. Our model is consistent with competitive binding of the two proteins, particularly at intermediate AU-balanced sequences. This suggests that fine changes in the A/U balance within a untranslated region (UTR) can alter the binding and subsequent stability of the message. Both feature engineering and domain adaptation emphasized the extent to which these proteins recognize similar general sequence features. This work suggests that the k-spectrum kernel method could be useful when studying RNA binding proteins and domain adaptation techniques such as feature augmentation could be employed particularly when examining RBPs with similar binding preferences. PMID:28333956

  1. Transcriptomic-Wide Discovery of Direct and Indirect HuR RNA Targets in Activated CD4+ T Cells.

    Directory of Open Access Journals (Sweden)

    Patsharaporn Techasintana

    Full Text Available Due to poor correlation between steady state mRNA levels and protein product, purely transcriptomic profiling methods may miss genes posttranscriptionally regulated by RNA binding proteins (RBPs and microRNAs (miRNAs. RNA immunoprecipitation (RIP methods developed to identify in vivo targets of RBPs have greatly elucidated those mRNAs which may be regulated via transcript stability and translation. The RBP HuR (ELAVL1 and family members are major stabilizers of mRNA. Many labs have identified HuR mRNA targets; however, many of these analyses have been performed in cell lines and oftentimes are not independent biological replicates. Little is known about how HuR target mRNAs behave in conditional knock-out models. In the present work, we performed HuR RIP-Seq and RNA-Seq to investigate HuR direct and indirect targets using a novel conditional knock-out model of HuR genetic ablation during CD4+ T activation and Th2 differentiation. Using independent biological replicates, we generated a high coverage RIP-Seq data set (>160 million reads that was analyzed using bioinformatics methods specifically designed to find direct mRNA targets in RIP-Seq data. Simultaneously, another set of independent biological replicates were sequenced by RNA-Seq (>425 million reads to identify indirect HuR targets. These direct and indirect targets were combined to determine canonical pathways in CD4+ T cell activation and differentiation for which HuR plays an important role. We show that HuR may regulate genes in multiple canonical pathways involved in T cell activation especially the CD28 family signaling pathway. These data provide insights into potential HuR-regulated genes during T cell activation and immune mechanisms.

  2. Intestinal epithelial HuR modulates distinct pathways of proliferation and apoptosis and attenuates small intestinal and colonic tumor development.

    Science.gov (United States)

    Giammanco, Antonina; Blanc, Valerie; Montenegro, Grace; Klos, Coen; Xie, Yan; Kennedy, Susan; Luo, Jianyang; Chang, Sung-Hee; Hla, Timothy; Nalbantoglu, Ilke; Dharmarajan, Sekhar; Davidson, Nicholas O

    2014-09-15

    HuR is a ubiquitous nucleocytoplasmic RNA-binding protein that exerts pleiotropic effects on cell growth and tumorigenesis. In this study, we explored the impact of conditional, tissue-specific genetic deletion of HuR on intestinal growth and tumorigenesis in mice. Mice lacking intestinal expression of HuR (Hur (IKO) mice) displayed reduced levels of cell proliferation in the small intestine and increased sensitivity to doxorubicin-induced acute intestinal injury, as evidenced by decreased villus height and a compensatory shift in proliferating cells. In the context of Apc(min/+) mice, a transgenic model of intestinal tumorigenesis, intestinal deletion of the HuR gene caused a three-fold decrease in tumor burden characterized by reduced proliferation, increased apoptosis, and decreased expression of transcripts encoding antiapoptotic HuR target RNAs. Similarly, Hur(IKO) mice subjected to an inflammatory colon carcinogenesis protocol [azoxymethane and dextran sodium sulfate (AOM-DSS) administration] exhibited a two-fold decrease in tumor burden. Hur(IKO) mice showed no change in ileal Asbt expression, fecal bile acid excretion, or enterohepatic pool size that might explain the phenotype. Moreover, none of the HuR targets identified in Apc(min/+)Hur(IKO) were altered in AOM-DSS-treated Hur(IKO) mice, the latter of which exhibited increased apoptosis of colonic epithelial cells, where elevation of a unique set of HuR-targeted proapoptotic factors was documented. Taken together, our results promote the concept of epithelial HuR as a contextual modifier of proapoptotic gene expression in intestinal cancers, acting independently of bile acid metabolism to promote cancer. In the small intestine, epithelial HuR promotes expression of prosurvival transcripts that support Wnt-dependent tumorigenesis, whereas in the large intestine epithelial HuR indirectly downregulates certain proapoptotic RNAs to attenuate colitis-associated cancer. Cancer Res; 74(18); 5322-35. ©2014 AACR.

  3. H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation.

    Directory of Open Access Journals (Sweden)

    Fu-Ming Tsai

    Full Text Available H-rev107 is a member of the HREV107 type II tumor suppressor gene family and acts as a phospholipase to catalyze the release of fatty acids from glycerophospholipid. H-rev107 has been shown to play an important role in fat metabolism in adipocytes through the PGE2/cAMP pathway, but the detailed molecular mechanism underlying H-rev107-mediated lipid degradation has not been studied. In this study, the interaction between H-rev107 and cytochrome P450 reductase (POR, which is involved in hepatic lipid content regulation, was determined by yeast two-hybrid screen and confirmed by using in vitro pull down assays and immunofluorescent staining. The expression of POR in H-rev107-expressing cells enhanced the H-rev107-mediated release of arachidonic acid. However, H-rev107 inhibited POR activity and relieved POR-mediated decreased triglyceride content in HtTA and HeLa cervical cells. The inhibitory effect of H-rev107 will be abolished when POR-expressing cells transfected with PLA2-lacking pH-rev107 or treated with PLA2 inhibitor. Silencing of H-rev107 using siRNA resulted in increased glycerol production and reversion of free fatty acid-mediated growth suppression in Huh7 hepatic cells. In summary, our results revealed that H-rev107 is also involved in lipid accumulation in liver cells through the POR pathway via its PLA2 activity.

  4. 胞质HuR在卵巢上皮性癌的表达及意义%The expression and significance of cytoplasmic HuR in epithelial ovarian carcinoma

    Institute of Scientific and Technical Information of China (English)

    齐玉明; 石彬; 郭皓; 成艳梅

    2011-01-01

    Objective To investigate the correlation between the expression of HuR in epithelial ovarian cancer and clinicopathological parameters in order to explore the factors that affect the prognosis of patients with epithelial ovarian carcinoma. Methods The expressions of cytoplasmic and nuclear HuR in normal ovary tissues,borderline tissue of ovary tumor and epithelial ovarian carcinoma were detected by SP immunohistochemical technique and RT-PCR. The relationship between cytoplasmic HuR expression and prognostic factors was analyzed.Results The expressions of cytoplasmic and nuclear HuR in epithelial ovarian cancer tissue were significantly higher than those in borderline tissue of epithelial ovarian tumor and normal ovarian tissues,however,there was no significant difference between the borderline tissue of epithelial ovarian tumor and normal ovarian tissue ( P >0.05). The statistical analysis showed that the positive expression rate of cytoplasmic HuR in epithelial ovarian carcinoma was closely correlated with histological grade and FIGO stage, but the positive expression rate of nuclear HuR was not correlated with the clinicopathological parameters. Conclusion The over-expression of HuR may play an important role in the growth and progress of epithelial ovarian carcinoma. To detect the expression of cytoplasmic HuR in epithelial ovarian carcinoma may predict the characteristics of oncobiology behaviour. The expression of cytoplasmic HuR may act as an independent prognostic indicator.%目的 检测HuR在卵巢上皮性癌中的表达及与临床病理参数的关系,寻求卵巢癌的预后因素.方法 采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法和逆转录RT-PCR技术,检测胞质和胞核HuR在正常、交界性、恶性卵巢组织中的表达;分析胞质HuR的表达与预后的关系.结果 胞质和胞核HuR在恶性卵巢肿瘤组织的表达明显高于交界性卵巢肿瘤和正常卵巢组织,胞质和胞核HuR在交界性

  5. Molecular interaction between K-Ras and H-REV107 in the Ras signaling pathway.

    Science.gov (United States)

    Han, Chang Woo; Jeong, Mi Suk; Jang, Se Bok

    2017-09-16

    Ras proteins are small GTPases that serve as master moderators of a large number of signaling pathways involved in various cellular processes. Activating mutations in Ras are found in about one-third of cancers. H-REV107, a K-Ras binding protein, plays an important role in determining K-Ras function. H-REV107 is a member of the HREV107 family of class II tumor suppressor genes and a growth inhibitory Ras target gene that suppresses cellular growth, differentiation, and apoptosis. Expression of H-REV107 was strongly reduced in about 50% of human carcinoma cell lines. However, the specific molecular mechanism by which H-REV107 inhibits Ras is still unknown. In the present study, we suggest that H-REV107 forms a strong complex with activating oncogenic mutation Q61H K-Ras from various biochemical binding assays and modeled structures. In addition, the interaction sites between K-Ras and H-REV107 were predicted based on homology modeling. Here, we found that some structure-based mutants of the K-Ras disrupted the complex formation with H-REV107. Finally, a novel molecular mechanism describing K-Ras and H-REV107 binding is suggested and insights into new K-Ras effector target drugs are provided. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Biological Characterization of CVRM2-BAC, A Recombinant CV1988 Virus Containing an REV LTR Insertion

    Science.gov (United States)

    It has been previously reported that avian retroviruses, i.e. avian leukosis virus (ALV) and reticoloendotheliosis virus (REV), integrate in the Marek’s disease virus genome affecting MDV pathogenicity. RM-2 is an attenuated serotype 1 MDV virus generated by insertion of the REV LTR in the genome of...

  7. 48 CFR 53.301-18 - SF 18 (Rev. 6/95), Request for Quotations.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false SF 18 (Rev. 6/95), Request for Quotations. 53.301-18 Section 53.301-18 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Illustrations of Forms 53.301-18 SF 18 (Rev. 6/95),...

  8. 48 CFR 53.301-1414 - SF 1414 (Rev. 10/93) Consent of Surety.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false SF 1414 (Rev. 10/93) Consent of Surety. 53.301-1414 Section 53.301-1414 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS Illustrations of Forms 53.301-1414 SF 1414 (Rev....

  9. Protective efficacy of a recombinant BAC clone of Marek's disease virus containing REV-LTR

    Science.gov (United States)

    Insertion of reticuloendotheliosis virus (REV) long-terminal repeat (LTR) into a bacterial artificial chromosome (BAC) clone of a very virulent strain of Marek’s disease (MD) virus (MDV), Md5 (Kim et al, 2011) rendered the resultant recombinant virus termed rMd5 REV-LTR BAC fully attenuated at passa...

  10. Pharmacological and Genetic Modulation of REV-ERB Activity and Expression Affects Orexigenic Gene Expression.

    Directory of Open Access Journals (Sweden)

    Ariadna Amador

    Full Text Available The nuclear receptors REV-ERBα and REV-ERBβ are transcription factors that play pivotal roles in the regulation of the circadian rhythm and various metabolic processes. The circadian rhythm is an endogenous mechanism, which generates entrainable biological changes that follow a 24-hour period. It regulates a number of physiological processes, including sleep/wakeful cycles and feeding behaviors. We recently demonstrated that REV-ERB-specific small molecules affect sleep and anxiety. The orexinergic system also plays a significant role in mammalian physiology and behavior, including the regulation of sleep and food intake. Importantly, orexin genes are expressed in a circadian manner. Given these overlaps in function and circadian expression, we wanted to determine whether the REV-ERBs might regulate orexin. We found that acute in vivo modulation of REV-ERB activity, with the REV-ERB-specific synthetic ligand SR9009, affects the circadian expression of orexinergic genes in mice. Long term dosing with SR9009 also suppresses orexinergic gene expression in mice. Finally, REV-ERBβ-deficient mice present with increased orexinergic transcripts. These data suggest that the REV-ERBs may be involved in the repression of orexinergic gene expression.

  11. PREFACE: International Symposium "Nanoscience and Quantum Physics 2011" (nanoPHYS'11)

    Science.gov (United States)

    Saito, Susumu; Tanaka, Hidekazu; Nakamura, Takashi; Nakamura, Masaaki

    2011-07-01

    Quantum physics has developed modern views of nature for more than a century. In addition to this traditional role, quantum physics has acquired new significance in the 21st century as the field responsible for driving and supporting nanoscience research, which will have even greater importance in the future because nanoscience will be the academic foundation for new technologies. The Department of Physics, Tokyo Institute of Technology, are now conducting a "Nanoscience and Quantum Physics" project (Physics G-COE project) supported by the Global Center of Excellence Program of the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) in order to promote research and education in these important academic fields. The International Symposium on Nanoscience and Quantum Physics, held in Tokyo, Japan, 26-28 January 2011 (nanoPHYS'11) was organized by the Physics G-COE project of the Tokyo Institute of Technology to provide an international forum for the open exchange of topical information and for stimulating discussion on novel concepts and future prospects of nanoscience and quantum physics. There were a total of 118 papers including 34 invited papers. This nanoPHYS'11 is the fourth symposium of this kind organized by the Tokyo Institute of Technology. Topics focused on in the symposium included: Category 1: Novel nanostructure (Nanowires, Nanotubes, Spin-related structure, etc) Category 2: Novel transport and electronic properties (Graphene, Topological insulators, Coherent control, etc) Category 3: Electronic and optical properties of nanostructure Category 4: Fundamental physics and new concept in quantum physics Category 5: Quantum Physics - Quantum information Category 6: Quantum Physics - Nuclear and Hadron Physics Category 7: Quantum Physics - Astrophysics, etc All the papers submitted to this issue have been reviewed under a stringent refereeing process, according to the normal rules of this Journal. The editors are grateful to all the

  12. HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3

    Energy Technology Data Exchange (ETDEWEB)

    Kula, Anna; Gharu, Lavina [The Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Padriciano 99, 34012 Trieste (Italy); Marcello, Alessandro, E-mail: marcello@icgeb.org [The Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Padriciano 99, 34012 Trieste (Italy)

    2013-01-20

    Human immunodeficiency virus gene expression and replication are regulated at several levels. Incompletely spliced viral RNAs and full-length genomic RNA contain the RRE element and are bound by the viral trans-acting protein Rev to be transported out of the nucleus. Previously we found that the nuclear matrix protein MATR3 was a cofactor of Rev-mediated RNA export. Here we show that the pleiotropic protein PSF binds viral RNA and is associated with MATR3. PSF is involved in the maintenance of a pool of RNA available for Rev activity. However, while Rev and PSF bind the viral pre-mRNA at the site of viral transcription, MATR3 interacts at a subsequent step. We propose that PSF and MATR3 define a novel pathway for RRE-containing HIV-1 RNAs that is hijacked by the viral Rev protein.

  13. Reversible HuR-microRNA binding controls extracellular export of miR-122 and augments stress response.

    Science.gov (United States)

    Mukherjee, Kamalika; Ghoshal, Bartika; Ghosh, Souvik; Chakrabarty, Yogaditya; Shwetha, Shivaprasad; Das, Saumitra; Bhattacharyya, Suvendra N

    2016-08-01

    microRNAs (miRNAs), the tiny but stable regulatory RNAs in metazoan cells, can undergo selective turnover in presence of specific internal and external cues to control cellular response against the changing environment. We have observed reduction in cellular miR-122 content, due to their accelerated extracellular export in human hepatic cells starved for small metabolites including amino acids. In this context, a new role of human ELAV protein HuR has been identified. HuR, a negative regulator of miRNA function, accelerates extracellular vesicle (EV)-mediated export of miRNAs in human cells. In stressed cells, HuR replaces miRNPs from target messages and is both necessary and sufficient for the extracellular export of corresponding miRNAs. HuR could reversibly bind miRNAs to replace them from Ago2 and subsequently itself gets freed from bound miRNAs upon ubiquitination. The ubiquitinated form of HuR is predominantly associated with multivesicular bodies (MVB) where HuR-unbound miRNAs also reside. These MVB-associated pool of miRNAs get exported out via EVs thereby delimiting cellular miR-122 level during starvation. Therefore, by modulating extracellular export of miR-122, HuR could control stress response in starved human hepatic cells.

  14. SU-E-T-470: Importance of HU-Mass Density Calibration Technique in Proton Pencil Beam Dose Calculation

    Energy Technology Data Exchange (ETDEWEB)

    Penfold, S; Miller, A [University of Adelaide, Adelaide, SA (Australia)

    2015-06-15

    Purpose: Stoichiometric calibration of Hounsfield Units (HUs) for conversion to proton relative stopping powers (RStPs) is vital for accurate dose calculation in proton therapy. However proton dose distributions are not only dependent on RStP, but also on relative scattering power (RScP) of patient tissues. RScP is approximated from material density but a stoichiometric calibration of HU-density tables is commonly neglected. The purpose of this work was to quantify the difference in calculated dose of a commercial TPS when using HU-density tables based on tissue substitute materials and stoichiometric calibrated ICRU tissues. Methods: Two HU-density calibration tables were generated based on scans of the CIRS electron density phantom. The first table was based directly on measured HU and manufacturer quoted density of tissue substitute materials. The second was based on the same CT scan of the CIRS phantom followed by a stoichiometric calibration of ICRU44 tissue materials. The research version of Pinnacle{sup 3} proton therapy was used to compute dose in a patient CT data set utilizing both HU-density tables. Results: The two HU-density tables showed significant differences for bone tissues; the difference increasing with increasing HU. Differences in density calibration table translated to a difference in calculated RScP of −2.5% for ICRU skeletal muscle and 9.2% for ICRU femur. Dose-volume histogram analysis of a parallel opposed proton therapy prostate plan showed that the difference in calculated dose was negligible when using the two different HU-density calibration tables. Conclusion: The impact of HU-density calibration technique on proton therapy dose calculation was assessed. While differences were found in the calculated RScP of bony tissues, the difference in dose distribution for realistic treatment scenarios was found to be insignificant.

  15. Altered regulation of ELAVL1/HuR in HLA-B27-expressing U937 monocytic cells.

    Directory of Open Access Journals (Sweden)

    Anna S Sahlberg

    Full Text Available OBJECTIVE: To investigate the role of HLA-B27 expression in the regulation of RNA binding protein (RBP Embryonic Lethal Abnormal Vision (ELAV L1/Human antigen R (HuR expression in Salmonella-infected or LPS-stimulated human monocytic cells, since HuR is a critical regulator of the post-transcriptional fate of many genes (e.g. TNFα important in inflammatory response. METHODS: U937 monocytic cells were stably transfected with pSV2neo resistant vector (mock, wild type HLA-B27, or mutated HLA-B27 with amino acid substitutions in the B pocket. Cells were differentiated, infected with Salmonella enteritidis or stimulated with lipopolysaccharide. The expression levels of HuR protein and cleavage products (CP1 and CP2 were detected by Western blotting and flow cytometry. Specific inhibitors were used to study the role of PKR and p38 in HuR expression and generation of CPs. TNFα and IL-10 secretion after p38 and PKR inhibition were measured by ELISA. RESULTS: Full length HuR is overexpressed and HuR cleavage is disturbed in U937 monocytic cells expressing HLA-B27 heavy chains (HC. Increased full length HuR expression, disturbed cleavage and reduced dependence on PKR after infection correlate with the expression of glutamic acid 45 in the B pocket that is linked to the misfolding of HLA-B27. CONCLUSION: Results show that the expression of HLA-B27 HCs modulates the intracellular environment of U937 monocyte/macrophages by altering HuR regulation. This phenomenon is at least partly dependent on the misfolding feature of the B27 molecule. Since HuR is an important regulator of multiple genes involved in inflammatory response observations offer an explanation how HLA-B27 may modulate inflammatory response.

  16. PyDecay/GraphPhys: A Unified Language and Storage System for Particle Decay Process Descriptions

    Energy Technology Data Exchange (ETDEWEB)

    Dunietz, Jesse N.; /MIT /SLAC

    2011-06-22

    To ease the tasks of Monte Carlo (MC) simulation and event reconstruction (i.e. inferring particle-decay events from experimental data) for long-term BaBar data preservation and analysis, the following software components have been designed: a language ('GraphPhys') for specifying decay processes, common to both simulation and data analysis, allowing arbitrary parameters on particles, decays, and entire processes; an automated visualization tool to show graphically what decays have been specified; and a searchable database storage mechanism for decay specifications. Unlike HepML, a proposed XML standard for HEP metadata, the specification language is designed not for data interchange between computer systems, but rather for direct manipulation by human beings as well as computers. The components are interoperable: the information parsed from files in the specification language can easily be rendered as an image by the visualization package, and conversion between decay representations was implemented. Several proof-of-concept command-line tools were built based on this framework. Applications include building easier and more efficient interfaces to existing analysis tools for current projects (e.g. BaBar/BESII), providing a framework for analyses in future experimental settings (e.g. LHC/SuperB), and outreach programs that involve giving students access to BaBar data and analysis tools to give them a hands-on feel for scientific analysis.

  17. The PhysTEC project: A perspective on what it takes to recruit and educate more physics teachers

    Science.gov (United States)

    Plisch, Monica

    2012-03-01

    The PhysTEC project has more than doubled the number of physics teachers educated at supported sites. These institutions were selected for their potential to implement change primarily in physics departments and build model teacher education programs. Key components of PhysTEC programs include active recruiting, early teaching experiences, pedagogical content knowledge, Learning Assistants, and induction and mentoring. Important structural elements include a program champion, a Teacher in Residence, assessment, collaboration, and institutional commitment. The PhysTEC project has supported about 20 institutions to date. In order to more fully address the national need for qualified physics teachers, the effort would need to be scaled up substantially. There is evidence of growing interest among physics departments in taking on this issue, and a national coalition committed to improving the education of future physics teachers has expanded to include more than 250 member institutions. The project is experimenting with targeted sites, funded at a lower level, to implement focused programs. In addition, PhysTEC is partnering with aligned efforts to magnify its impact. PhysTEC is a project led by APS with AAPT, and supported by the NSF and the APS Campaign for the 21st Century.

  18. Central hypoventilation as the presenting symptom in Hu associated paraneoplastic encephalomyelitis.

    Science.gov (United States)

    Gómez-Choco, Manuel J; Zarranz, Juan J; Saiz, Albert; Forcadas, María I; Graus, Francesc

    2007-10-01

    Central hypoventilation is usually caused by ischaemic or neoplastic lesions of the medulla and upper cervical spinal cord. An autoimmune disorder is not usually considered in the differential diagnosis of this syndrome. We retrospectively identified 14 patients from our database of 202 patients with Hu antibodies who presented with brainstem symptoms. Three were admitted to hospital because of central hypoventilation. All underwent intubation and mechanical ventilation. They could breathe properly while they were awake but suffered deep apnoeas during sleep. Two died, but one is still alive requiring ventilatory assistance during sleep. Autopsy was performed in one of the patients which showed severe inflammatory infiltrates and neuronal loss in the medulla. All patients had normal brain imaging studies and the cause of central hypoventilation was an unsolved problem until Hu antibodies were determined.

  19. Reply to “Comment on ‘Gauge invariance and k-factorization of exclusive processes’ [Phys. Lett. B 674 (2009) 176]” [Phys. Lett. B 674 (2009) 182

    Science.gov (United States)

    Feng, F.; Ma, J. P.; Wang, Q.

    2009-06-01

    A new method is proposed to calculate wave functions in kT-factorization in [H.-n. Li, S. Mishima, Phys. Lett. B 674 (2009) 182, arXiv:0808.1526] as a comment about our paper [F. Feng, J.P. Ma, Q. Wang, Phys. Lett. B 674 (2009) 176, arXiv:0807.0296]. We point out that the results obtained with the method are in conflict with the translation invariance and depend on the chosen contours for loop-integrals. Therefore, the method is in principle unacceptable and the results with the method cannot be correct.

  20. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells

    Directory of Open Access Journals (Sweden)

    Xiao-Hong Li

    2013-01-01

    Full Text Available Calcium signals in hepatocytes control cell growth, proliferation, and death. Members of the transient receptor potential (TRP cation channel superfamily are candidate calcium influx channels. NFκB activation strictly depends on calcium influx and often induces antiapoptotic genes favouring cell survival. Previously, we reported that S-[6]-gingerol is an efficacious agonist of the transient receptor potential cation channel subfamily V member 1 (TRPV1 in neurones. In this study, we tested the effect of S-[6]-gingerol on HuH-7 cells using the Fluo-4 calcium assay, RT-qPCR, transient cell transfection, and luciferase measurements. We found that S-[6]-gingerol induced a transient rise in [Ca2+]i in HuH-7 cells. The increase in [Ca2+]i induced by S-[6]-gingerol was abolished by preincubation with EGTA and was also inhibited by the TRPV1 channel antagonist capsazepine. Expression of TRPV1 in HuH-7 cells was confirmed by mRNA analysis as well as a test for increase of [Ca2+]i by TRPV1 agonist capsaicin and its inhibition by capsazepine. We found that S-[6]-gingerol induced rapid NFκB activation through TRPV1 in HuH-7 cells. Furthermore, S-[6]-gingerol-induced NFκB activation was dependent on the calcium gradient and TRPV1. The rapid NFκB activation by S-[6]-gingerol was associated with an increase in mRNA levels of NFκB-target genes: cIAP-2, XIAP, and Bcl-2 that encode antiapoptotic proteins.

  1. Effects of animal class and genotype on beef muscle nanostructure, pHu, colour and tenderness

    Directory of Open Access Journals (Sweden)

    Amanda Y. Chulayo

    2016-07-01

    Full Text Available The objective of the study was to determine the effects of animal class and genotype of cattle on Muscularis longissimus thoracis et lumborum (LTL nanostructure, ultimate pH (pHu, colour and tenderness of beef. We found significant positive relationships between distance travelled (DT and meat temperature (Tm (p"less than"0.01; lairage duration (LDhr and lightness of colour (L* (p"less than"0.01; ambient temperature (Ta and L* (p"less than"0.05 and LDhr and yellowness (b* (p"less than"0.05 of beef from Bonsmara cattle. Positive linear relationships were observed between DT and Tm (p"less than"0.05 and DT and L* (p"less than"0.01 of the non-descript cattle. There were no significant relationships between pre-slaughter stress and other beef quality parameters (pHu, Warner– Bratzler shear force [WBSF], redness [a*] and b* of Bonsmara, Nguni and non-descript cattle. Muscle fibres differed among class and genotype and had an effect on meat quality. The Bonsmara, non-descript and Nguni cows and heifers had visible skeletal muscle fibres which were thin and long, promising improved tenderness of beef. Genotype and class had significant effects on meat quality parameters (Tm, pHu, L*, a*, b* and WBSF. The first important principal components as they appeared from the analysis were pHu, Tm, L*, a*, b* and WBSF. Therefore, animal class did not affect the nanostructure of beef; instead, meat tenderness was enhanced by the longer and visible muscle fibres. Nguni cattle produced meat of superior quality to that of the Bonsmara and the non-descript cattle.

  2. The outcome of PIE *#Hi- and *#Hu- in Germanic

    DEFF Research Database (Denmark)

    Hansen, Bjarne Simmelkjær Sandgaard

    2015-01-01

    It has been established with a great amount of certainty that PIE *#Hu- > Gr. #Vὐ- and that PIE *#Hi- > Gr. #ἰ-. It still remains to be demonstrated what happens in other Western Indo-European branches, including Germanic. In this article , I reject the statement by Ringe (1988: 433) that PIE *#H...... tentatively propose that PG *#au- > PG *#u- when followed by a labial consonant; a development partially paralleled in Greek and in English....

  3. Sustainable groundwater development and management in the Quaternary Hang-Jia-Hu Plain, China

    Institute of Scientific and Technical Information of China (English)

    朱琰

    2002-01-01

    Based on the results of study on regional water supply system, water quality assessments, Quaternary aquifers investigation, and correlation analysis of groundwater depression resulting from land subsidence in the Hang-Jia-Hu Quaternary Plain, this paper presents the groundwater resources policy and sustainable management methods suitable for this area. Suggestions for controlling land subsidence by implementation of wise groundwater policy and management measures are also given.

  4. The outcome of PIE *#Hi- and *#Hu- in Germanic

    DEFF Research Database (Denmark)

    Hansen, Bjarne Simmelkjær Sandgaard

    2015-01-01

    It has been established with a great amount of certainty that PIE *#Hu- > Gr. #Vὐ- and that PIE *#Hi- > Gr. #ἰ-. It still remains to be demonstrated what happens in other Western Indo-European branches, including Germanic. In this article , I reject the statement by Ringe (1988: 433) that PIE *#H...... tentatively propose that PG *#au- > PG *#u- when followed by a labial consonant; a development partially paralleled in Greek and in English....

  5. Screening candidate microRNAs (miRNAs) in different lambskin hair follicles in Hu sheep.

    Science.gov (United States)

    Gao, Wen; Sun, Wei; Yin, Jinfeng; Lv, Xiaoyang; Bao, Jianjun; Yu, Jiarui; Wang, Lihong; Jin, Chengyan; Hu, Liang

    2017-01-01

    Hu sheep lambskin is a unique white lambskin from China that exhibits three types of flower patterns, including small waves, medium waves, and large waves, with small waves considered the best quality. However, our understanding of the molecular mechanism underlying flower pattern formation in Hu sheep lambskin is limited. The aim of the present study was to further explore the relevance between candidate microRNAs (miRNAs) and developmental characteristics of hair follicles and screen miRNAs for later functional validation. Herein, we employed Illumina Hiseq 2500 to identify differentially expressed miRNAs in hair follicles of different flower patterns with small, medium, and large waves to construct a comprehensive sequence database on the mechanism of hair follicle development. Paraffin sections of lambskin tissue were prepared to assess the structure of different hair follicles. Expression levels of candidate miRNAs in different flower patterns were analyzed by relative quantitation using real-time PCR, combined with histological observation and micro-observation technologies, and the correlation between expression levels of candidate miRNAs and histological properties of hair follicles was analyzed by using SPSS 17.0. A total of 522 differentially expressed miRNAs were identified, and RNA-seq analysis detected 7,266 target genes in different groups of flower patterns. Gene ontological analysis indicated these target genes were mainly involved in cell proliferation, differentiation, growth, apoptosis, and ion transport, and 14 miRNAs, including miR-143, miR-10a, and let-7 were screened as candidate miRNAs in Hu sheep hair follicle growth and development. In the same field of vision, variance analysis showed that the number of secondary follicles in small waves was significantly larger than that in large and medium waves (Phair follicles, highly significant differences in miRNA-143 expression levels between large and small waves were observed (Phair follicle

  6. Downregulation of β-Adrenoceptors in Isoproterenol-Induced Cardiac Remodeling through HuR.

    Directory of Open Access Journals (Sweden)

    Qian Yin

    Full Text Available β-adrenergic receptors (β-ARs play an important role in cardiac remodeling, which is the key pathological process in various heart diseases and leads to heart failure. However, the regulation of β-AR expression in remodeling hearts is still unclear. This study aims to clarify the possible mechanisms underlying the regulation of β1- and β2-AR expression in cardiac remodeling. The rat model of cardiac remodeling was established by subcutaneous injection of isoproterenol(ISO at the dose of 0.25 mg·kg(-1·d(-1 for 7 days. We found that the expression of β1- and β2-ARs decreased in the remodeling heart. The mechanisms may include the inhibition of DNA transcription and the increase of mRNA degradation. cAMP-response element binding protein(CREB is a well-known transcription factor of β-AR. However, the expression and activation of CREB was not changed in the remodeling heart. Further, human Antigen-R (HuR, a RNA binding protein, which binds to the 3'-untranslated region of the β-AR mRNA and promotes RNA degradation, was increased in the remodeling model. And in vitro, HuR deficiency reversed the reduction of β-AR mRNA induced by ISO. Therefore, the present findings indicate that HuR, but not CREB, is responsible for the reduction of β-AR expression in ISO induced cardiac remodeling.

  7. Intraperirhinal cortex administration of the synthetic cannabinoid, HU210, disrupts object recognition memory in rats.

    Science.gov (United States)

    Sticht, Martin A; Jacklin, Derek L; Mechoulam, Raphael; Parker, Linda A; Winters, Boyer D

    2015-03-25

    Cannabinoids disrupt learning and memory in human and nonhuman participants. Object recognition memory, which is particularly susceptible to the impairing effects of cannabinoids, relies critically on the perirhinal cortex (PRh); however, to date, the effects of cannabinoids within PRh have not been assessed. In the present study, we evaluated the effects of localized administration of the synthetic cannabinoid, HU210 (0.01, 1.0 μg/hemisphere), into PRh on spontaneous object recognition in Long-Evans rats. Animals received intra-PRh infusions of HU210 before the sample phase, and object recognition memory was assessed at various delays in a subsequent retention test. We found that presample intra-PRh HU210 dose dependently (1.0 μg but not 0.01 μg) interfered with spontaneous object recognition performance, exerting an apparently more pronounced effect when memory demands were increased. These novel findings show that cannabinoid agonists in PRh disrupt object recognition memory.

  8. 论胡锦涛的人才观%On HuJintao' s View of Talent

    Institute of Scientific and Technical Information of China (English)

    于伟峰; 黄静

    2012-01-01

    胡锦涛的人才观主要体现在:树立人才资源为第一资源、人人皆可成才和以人为本的观念。胡锦涛的人才观的核心内容与前三代领导集体人才观的核心内容既一眯相承又有其独特的创新点,他首次提出了党管人才的原则和将人才观与以人为本的发展观相结合的原则。%Hu Jintao' s view of talents is embodied in 1 ) his view that human resources are paramount to all other resources; 2) his belief that everyone can develop into a man of caliber; and 3) his people-oriented approach to development. The kernel of Hu Jintao' s view of talent is imbued with the same spirit reflected in the general out- look on talent of previous three generations of central collective leadership of the CPC, while boasting unique inno- vations. Hu first puts forward the principle of managing talents by the party and the principle of combining the sci- entific view of talent with the people-oriented approach to development.

  9. Anti-solar differential rotation on the active sub-giant HU Virginis

    CERN Document Server

    Harutyunyan, G; Künstler, A; Carroll, T A; Weber, M

    2016-01-01

    Measuring surface differential rotation (DR) on different types of stars is important when characterizing the underlying stellar dynamo. It has been suggested that anti-solar DR laws can occur when strong meridional flows exist. We aim to investigate the differential surface rotation on the primary star of the RS CVn binary HU Vir by tracking its starspot distribution as a function of time. We also aim to recompute and update the values for several system parameters of the triple system HU Vir (close and wide orbits). Time-series high-resolution spectroscopy for four continuous months was obtained with the 1.2-m robotic STELLA telescope. Nine consecutive Doppler images were reconstructed from these data, using our line-profile inversion code iMap. An image cross-correlation method was applied to derive the surface differential-rotation law for HU Vir. New orbital elements for the close and the wide orbits were computed using our new STELLA radial velocities (RVs) combined with the RV data available in the lit...

  10. CTXφ Replication Depends on the Histone-Like HU Protein and the UvrD Helicase.

    Directory of Open Access Journals (Sweden)

    Eriel Martínez

    2015-05-01

    Full Text Available The Vibrio cholerae bacterium is the agent of cholera. The capacity to produce the cholera toxin, which is responsible for the deadly diarrhea associated with cholera epidemics, is encoded in the genome of a filamentous phage, CTXφ. Rolling-circle replication (RCR is central to the life cycle of CTXφ because amplification of the phage genome permits its efficient integration into the genome and its packaging into new viral particles. A single phage-encoded HUH endonuclease initiates RCR of the proto-typical filamentous phages of enterobacteriaceae by introducing a nick at a specific position of the double stranded DNA form of the phage genome. The rest of the process is driven by host factors that are either essential or crucial for the replication of the host genome, such as the Rep SF1 helicase. In contrast, we show here that the histone-like HU protein of V. cholerae is necessary for the introduction of a nick by the HUH endonuclease of CTXφ. We further show that CTXφ RCR depends on a SF1 helicase normally implicated in DNA repair, UvrD, rather than Rep. In addition to CTXφ, we show that VGJφ, a representative member of a second family of vibrio integrative filamentous phages, requires UvrD and HU for RCR while TLCφ, a satellite phage, depends on Rep and is independent from HU.

  11. Cannabinoids & Stress: impact of HU-210 on behavioral tests of anxiety in acutely stressed mice.

    Science.gov (United States)

    Kinden, Renee; Zhang, Xia

    2015-05-01

    Anxiety disorders are one of the most prevalent classes of mental disorders affecting the general population, but current treatment strategies are restricted by their limited efficacy and side effect profiles. Although the cannabinoid system is speculated to be a key player in the modulation of stress responses and emotionality, the vast majority of current research initiatives had not incorporated stress exposure into their experimental designs. This study was the first to investigate the impact of exogenous cannabinoid administration in an acutely stressed mouse model, where CD1 mice were pre-treated with HU-210, a potent CB1R agonist, prior to acute stress exposure and subsequent behavioral testing. Exogenous cannabinoid administration induced distinct behavioral phenotypes in stressed and unstressed mice. While low doses of HU-210 were anxiolytic in unstressed subjects, this effect was abolished when mice were exposed to an acute stressor. The administration of higher HU-210 doses in combination with acute stress exposure led to severe locomotor deficits that were not previously observed at the same dose in unstressed subjects. These findings suggest that exogenous cannabinoids and acute stress act synergistically in an anxiogenic manner. This study underlies the importance of including stress exposure into future anxiety-cannabinoid research due to the differential impact of cannabinoid administration on stressed and unstressed subjects.

  12. Clinical study on intravitreal injection of cannabinoid HU-211 for optic nerve damage in glaucoma rats

    Directory of Open Access Journals (Sweden)

    Hui-Feng Liu

    2014-09-01

    Full Text Available AIM: To evaluate the protective effects of intravitreal injection of cannabinoid HU-211 for optic nerve in glaucoma rats, providing the experimental evidence in the treatment of glaucoma optic nerve injury.METHODS: Glaucoma rats(18 eyeswere modeled by electric coagulation sclera surface vein and randomized into 3 groups, group A received intravitreal injection of 1mg/0.1mL cannabinoid HU-211 every other day respectively; group B was given intravitreal injection of 0.1mL water every other day, group C was high intraocular pressure(IOPgroup, 6 eyes were randomly selected for blank control group(group D. IOP was observed every day. The rats were sacrificed after treatment 4wk, froze retina section, HE stain. The density fluctuation of retinal ganglion cell(RGCneurons assessment the optic nerve of rat model with chronic high IOP glaucoma were measured.RESULTS: The apoptosis and damage degree of RGC in group B was obviously higher than that in group A, with statistically significant difference(PP >0.05.CONCLUSION: Intravitreal injection of cannabinoid HU-211 shows obvious protective effect on optic nerve in glaucom rat models.

  13. Rev1 is a base excision repair enzyme with 5′-deoxyribose phosphate lyase activity

    Science.gov (United States)

    Prasad, Rajendra; Poltoratsky, Vladimir; Hou, Esther W.; Wilson, Samuel H.

    2016-01-01

    Rev1 is a member of the Y-family of DNA polymerases and is known for its deoxycytidyl transferase activity that incorporates dCMP into DNA and its ability to function as a scaffold factor for other Y-family polymerases in translesion bypass events. Rev1 also is involved in mutagenic processes during somatic hypermutation of immunoglobulin genes. In light of the mutation pattern consistent with dCMP insertion observed earlier in mouse fibroblast cells treated with a base excision repair-inducing agent, we questioned whether Rev1 could also be involved in base excision repair (BER). Here, we uncovered a weak 5′-deoxyribose phosphate (5′-dRP) lyase activity in mouse Rev1 and demonstrated the enzyme can mediate BER in vitro. The full-length Rev1 protein and its catalytic core domain are similar in their ability to support BER in vitro. The dRP lyase activity in both of these proteins was confirmed by NaBH4 reduction of the Schiff base intermediate and kinetics studies. Limited proteolysis, mass spectrometry and deletion analysis localized the dRP lyase active site to the C-terminal segment of Rev1's catalytic core domain. These results suggest that Rev1 could serve as a backup polymerase in BER and could potentially contribute to AID-initiated antibody diversification through this activity. PMID:27683219

  14. Structural model of the Rev regulatory protein from equine infectious anemia virus.

    Directory of Open Access Journals (Sweden)

    Yungok Ihm

    Full Text Available Rev is an essential regulatory protein in the equine infectious anemia virus (EIAV and other lentiviruses, including HIV-1. It binds incompletely spliced viral mRNAs and shuttles them from the nucleus to the cytoplasm, a critical prerequisite for the production of viral structural proteins and genomic RNA. Despite its important role in production of infectious virus, the development of antiviral therapies directed against Rev has been hampered by the lack of an experimentally-determined structure of the full length protein. We have used a combined computational and biochemical approach to generate and evaluate a structural model of the Rev protein. The modeled EIAV Rev (ERev structure includes a total of 6 helices, four of which form an anti-parallel four-helix bundle. The first helix contains the leucine-rich nuclear export signal (NES. An arginine-rich RNA binding motif, RRDRW, is located in a solvent-exposed loop region. An ERLE motif required for Rev activity is predicted to be buried in the core of modeled structure where it plays an essential role in stabilization of the Rev fold. This structural model is supported by existing genetic and functional data as well as by targeted mutagenesis of residues predicted to be essential for overall structural integrity. Our predicted structure should increase understanding of structure-function relationships in Rev and may provide a basis for the design of new therapies for lentiviral diseases.

  15. Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation

    Directory of Open Access Journals (Sweden)

    Jun Takezawa

    2010-01-01

    Full Text Available When a replicative DNA polymerase stalls upon encountering a lesion on the template strand, it is relieved by other low-processivity polymerase(s, which insert nucleotide(s opposite the lesion, extend by a few nucleotides, and dissociate from the 3′-OH. The replicative polymerase then resumes DNA synthesis. This process, termed translesion replication (TLS or replicative bypass, may involve at least five different polymerases in mammals, although the participating polymerases and their roles have not been entirely characterized. Using siRNAs originally designed and an alkaline sucrose density gradient sedimentation technique, we verified the involvement of several polymerases in ultraviolet (UV light-induced TLS in HeLa cells. First, siRNAs to Rev3 or Rev7 largely abolished UV-TLS, suggesting that these 2 gene products, which comprise Polζ, play a main role in mutagenic TLS. Second, Rev1-targeted siRNA also abrogated UV-TLS, indicating that Rev1 is also indispensable to mutagenic TLS. Third, Polη-targeted siRNA also prevented TLS to a greater extent than our expectations. Forth, although siRNA to Polι had no detectable effect, that to Polκ delayed UV-TLS. To our knowledge, this is the first study reporting apparent evidence for the participation of Polκ in UV-TLS.

  16. PhysTrack’: a Matlab based environment for video tracking of kinematics in the physics laboratory

    Science.gov (United States)

    Umar Hassan, Muhammad; Sabieh Anwar, Muhammad

    2017-07-01

    In the past two decades, several computer software tools have been developed to investigate the motion of moving bodies in physics laboratories. In this article we report a Matlab based video tracking library, PhysTrack, primarily designed to investigate kinematics. We compare PhysTrack with other commonly available video tracking tools and outline its salient features. The general methodology of the whole video tracking process is described with a step by step explanation of several functionalities. Furthermore, results of some real physics experiments are also provided to demonstrate the working of the automated video tracking, data extraction, data analysis and presentation tools that come with this development environment. We believe that PhysTrack will be valuable for the large community of physics teachers and students already employing Matlab.

  17. The cytoskeletal inhibitors latrunculin A and blebbistatin exert antitumorigenic properties in human hepatocellular carcinoma cells by interfering with intracellular HuR trafficking

    Energy Technology Data Exchange (ETDEWEB)

    Doller, Anke; Badawi, Amel [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Schmid, Tobias; Brauß, Thilo [Institut für Biochemie I (Pathobiochemie), Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Pleli, Thomas [Medizinische Klinik 1, Schwerpunkt Gastroenterologie und Hepatologie, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Meyer zu Heringdorf, Dagmar [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Piiper, Albrecht [Medizinische Klinik 1, Schwerpunkt Gastroenterologie und Hepatologie, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Pfeilschifter, Josef [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Eberhardt, Wolfgang, E-mail: w.eberhardt@em.uni-frankfurt.de [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany)

    2015-01-01

    The impact of the RNA-binding protein HuR for the post-transcriptional deregulation of tumor-relevant genes is well established. Despite of elevations in HuR expression levels, an increase in cytoplasmic HuR abundance in many cases correlates with a high grade of malignancy. Here, we demonstrated that administration of the actin-depolymerizing macrolide latrunculin A, or blebbistatin, an inhibitor of myosin II ATPase activity, caused a dose- and time-dependent reduction in the high cytoplasmic HuR content of HepG2 and Huh7 hepatocellular carcinoma (HCC) cells. Subcellular fractionation revealed that in addition, both inhibitors strongly attenuated cytoskeletal and membrane-bound HuR abundance and conversely increased the HuR amount in nuclear cell fractions. Concomitant with changes in intracellular HuR localization, both cytoskeletal inhibitors markedly decreased the half-lives of cyclooxygenase-2 (COX-2), cyclin A and cyclin D{sub 1} encoding mRNAs resulting in a significant reduction in their expression levels in HepG2 cells. Importantly, a similar reduction in the expression of these HuR targets was achieved by a RNA interference (RNAi)-mediated knockdown of either HuR or nonmuscle myoin IIA. Using polysomal fractionation, we further demonstrate that the decrease in cytoplasmic HuR by latrunculin A or blebbistatin is accompanied by a marked change in the allocation of HuR and its mRNA cargo from polysomes to ribonucleoprotein (RNP) particles. Functionally, the basal migration and prostaglandin E{sub 2} synthesis are similarly impaired in inhibitor-treated and stable HuR-knockdown HepG2 cells. Our data demonstrate that interfering with the actomyosin-dependent HuR trafficking may comprise a valid therapeutic option for antagonizing pathologic posttranscriptional gene expression by HuR and furthermore emphasize the potential benefit of HuR inhibitory strategies for treatment of HCC. - Highlights: • We tested the effects of latrunculin A and blebbistatin on

  18. High-level Expression and Purification of Human Zona Pellucida huZP3a22-176 and huZP3b177-348 Peptides in Escherichia coli

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Objective To try making huZP3a22-176 and huZP3b177-348 polypeptides (representing an intact huZP322-348 protein without its N-terminal signal peptide and C-terminal transmembrane domain ) express in E. coli at a higher levelMethods The cDNAs encoding huZP3a and huZP3b were obtained with PCR method.The pB V221 plasmid was used to construct thermo-inducible recombinant expression vector. Purification of two target expression products employed an improved method of preparative gel polyacrylamide gel electrophoresis.Results Two polypeptides of recombinant huZP3a (rhuZP3a) and recombinant huZP3b(rhuZP3b) were all expressed respectively in an E. coli BL21(DE3)pLysS strain at a higher level, which were recognized by two specific polyclonal antisera in Western blotting test which recognize a linear B cell epitope present in rhuZP3a or rhuZP3b respectively. Using the shake-flask method, approximately 5 mg of rhuZP3a and rhuZP3b with more than 95% relative homogeneity were harvested from 1 L culture respectively.Conclusion The availability of two rhuZP3 polypeptides will help in detecting the immunogenicities of rhuZP3a and rhuZP3b through animal experiments and confirming the function domain of non-glycosylated huZP3 to induce acrosome reaction in vitro.

  19. Rev-erb beta regulates the Srebp-1c promoter and mRNA expression in skeletal muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Ramakrishnan, Sathiya N.; Lau, Patrick; Crowther, Lisa M. [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia); Cleasby, Mark E. [Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St. Vincent' s Hospital, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 (Australia); Millard, Susan; Leong, Gary M. [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia); Cooney, Gregory J. [Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St. Vincent' s Hospital, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 (Australia); Muscat, George E.O., E-mail: g.muscat@imb.uq.edu.au [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia)

    2009-10-30

    The nuclear hormone receptor, Rev-erb beta operates as a transcriptional silencer. We previously demonstrated that exogenous expression of Rev-erb{beta}{Delta}E in skeletal muscle cells increased Srebp-1c mRNA expression. We validated these in vitro observations by injection of an expression vector driving Rev-erb{beta}{Delta}E expression into mouse tibialis muscle that resulted in increased Srebp-1c mRNA expression. Paradoxically, Rev-erb{beta} siRNA expression in skeletal muscle cells repressed Srebp-1c expression, and indicated that Rev-erb{beta} expression was necessary for Srebp-1c expression. ChIP analysis demonstrated that Rev-erb{beta} was recruited to the Srebp-1c promoter. Moreover, Rev-erb{beta} trans-activated the Srebp-1c promoter, in contrast, Rev-erb{beta} efficiently repressed the Rev-erb{alpha} promoter, a previously characterized target gene. Finally, treatment with the Rev-erb agonist (hemin) (i) increased the trans-activation of the Srebp-1c promoter by Rev-erb{beta}; and (ii) increased Rev-erb{beta} and Srebp-1c mRNA expression. These data suggest that Rev-erb{beta} has the potential to activate gene expression, and is a positive regulator of Srebp-1c, a regulator of lipogenesis.

  20. Theoretical Study of Solid State Quantum Information Processing

    Science.gov (United States)

    2013-08-28

    Physical Review A, (02 2013): 0. doi: 10.1103/PhysRevA.87.022332 08/28/2013 30.00 Peihao Huang, Xuedong Hu. Spin qubit relaxation in a moving quantum dot, Physical Review B, (08 2013): 0. doi: 10.1103/PhysRevB.88.075301 08/28/2013 29.00 Lukasz Cywinski, Xuedong Hu, S. Das Sarma, Jo-Tzu Hung. Hyperfine interaction induced dephasing of coupled spin qubits in semiconductor double quantum dots, Physical Review B, (08 2013): 0. doi: 10.1103/PhysRevB.88.085314 08/28/2013 28.00 Ting Yu, WenXian Zhang, XueDong Hu,

  1. Comment on “Temperature dependence of atomic vibrations in mono-layer graphene” [J. Appl. Phys. 118, 074302 (2015)

    Energy Technology Data Exchange (ETDEWEB)

    Susi, T., E-mail: toma.susi@univie.ac.at; Kotakoski, J. [Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna (Austria)

    2016-02-14

    In an interesting recent study [Allen et al., J. Appl. Phys. 118, 074302 (2015)] (see also their Erratum [Allen et al., J. Appl. Phys. 118, 159902 (2015)]), Allen and co-workers measured the mean square amplitudes of graphene lattice vibrations between 100 and 1300 K and used a simplified theoretical approximation for the acoustic phonon modes to evaluate the maximum phonon wavelengths supported by the lattice. By fitting their data using the smallest wave-vector as the fitting parameter, they found this to be significantly smaller than the physical size of the graphene crystallites.

  2. Isolation, purification, crystallization, and preliminary X-ray diffraction study of the crystals of HU protein from M. gallisepticum

    Science.gov (United States)

    Nikolaeva, A. Yu.; Timofeev, V. I.; Boiko, K. M.; Korzhenevskii, D. A.; Rakitina, T. V.; Dorovatovskii, P. V.; Lipkin, A. V.

    2015-11-01

    HU proteins are involved in bacterial DNA and RNA repair. Since these proteins are absent in cells of higher organisms, inhibitors of HU proteins can be used as effective and safe antibiotics. The crystallization conditions for the M. gallisepticum HU protein were found and optimized by the vapor-diffusion method. The X-ray diffraction data set was collected to 2.91 Å resolution from the crystals grown by the vapor-diffusion method on a synchrotron source. The crystals of the HU protein belong to sp. gr. P41212 and have the following unit-cell parameters: a = b = 97.94 Å, c = 77.92 Å, α = β = γ = 90°.

  3. Rev-Erb co-regulates muscle regeneration via tethered interaction with the NF-Y cistrome

    Directory of Open Access Journals (Sweden)

    Ryan D. Welch

    2017-07-01

    Conclusions: Disrupting Rev-Erb activity in injured muscle accelerates regenerative muscle repair/differentiation through transcriptional de-repression of myogenic programs. Rev-Erb, therefore, may be a potent therapeutic target for a myriad of muscular disorders.

  4. HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells

    Directory of Open Access Journals (Sweden)

    Ennifar Eric

    2008-06-01

    Full Text Available Abstract Reverse transcription of the genetic material of human immunodeficiency virus type 1 (HIV-1 is a critical step in the replication cycle of this virus. This process, catalyzed by reverse transcriptase (RT, is well characterized at the biochemical level. However, in infected cells, reverse transcription occurs in a multiprotein complex – the reverse transcription complex (RTC – consisting of viral genomic RNA associated with viral proteins (including RT and, presumably, as yet uncharacterized cellular proteins. Very little is known about the cellular proteins interacting with the RTC, and with reverse transcriptase in particular. We report here that HIV-1 reverse transcription is affected by the levels of a nucleocytoplasmic shuttling protein – the RNA-binding protein HuR. A direct protein-protein interaction between RT and HuR was observed in a yeast two-hybrid screen and confirmed in vitro by homogenous time-resolved fluorescence (HTRF. We mapped the domain interacting with HuR to the RNAse H domain of RT, and the binding domain for RT to the C-terminus of HuR, partially overlapping the third RRM RNA-binding domain of HuR. HuR silencing with specific siRNAs greatly impaired early and late steps of reverse transcription, significantly inhibiting HIV-1 infection. Moreover, by mutagenesis and immunoprecipitation studies, we could not detect the binding of HuR to the viral RNA. These results suggest that HuR may be involved in and may modulate the reverse transcription reaction of HIV-1, by an as yet unknown mechanism involving a protein-protein interaction with HIV-1 RT.

  5. Domain-specific phosphomimetic mutation allows dissection of different protein kinase C (PKC) isotype-triggered activities of the RNA binding protein HuR.

    Science.gov (United States)

    Schulz, Sebastian; Doller, Anke; Pendini, Nicole R; Wilce, Jacqueline A; Pfeilschifter, Josef; Eberhardt, Wolfgang

    2013-12-01

    The ubiquitous mRNA binding protein human antigen R (HuR) participates in the post-transcriptional regulation of many AU-rich element (ARE)-bearing mRNAs. Previously, by using in vitro kinase assay, we have identified serines (Ser) 158, 221 and 318 as targets of protein kinase C (PKC)-triggered phosphorylation. In this study, we tested whether GFP- or GST-tagged HuR constructs bearing a phosphomimetic Ser (S)-to-Asp (D) substitution at the different PKC target sites, would affect different HuR functions including HuR nucleo-cytoplasmic redistribution and binding to different types of ARE-containing mRNAs. The phosphomimetic GFP-tagged HuR protein bearing a phosphomimetic substitution in the hinge region of HuR (HuR-S221D) showed an increased cytoplasmic abundance when compared to wild-type HuR. Conversely, data from in vitro kinase assay and electrophoretic mobility shift assay (EMSA), implicates that phosphorylation at Ser 221 is not relevant for mRNA binding of HuR. Quantification of in vitro binding affinities of GST-tagged wild-type HuR and corresponding HuR proteins bearing a phosphomimetic substitution in either RRM2 (HuR-S158D) or in RRM3 (HuR-S318D) by microscale thermophoresis (MST) indicates a specific binding of wild-type HuR to type I, II or type III-ARE-oligonucleotides in the high nanomolar range. Interestingly, phosphomimetic mutation at position 158 or 318 had a negative influence on HuR binding to type I- and type II-ARE-mRNAs whereas it significantly enhanced HuR affinity to a type III-ARE substrate. Our data suggest that differential phosphorylation of HuR by PKCs at different HuR domains coordinates subcellular HuR distribution and leads to a preferential binding to U-rich bearing target mRNA.

  6. A Review of the Pharmacological Effects of the Dried Root of Polygonum cuspidatum (Hu Zhang and Its Constituents

    Directory of Open Access Journals (Sweden)

    Huan Zhang

    2013-01-01

    Full Text Available Traditional Chinese medicine (TCM has been widely used in China for thousands of years to treat and prevent diseases. TCM has been proven safe and effective, and it is being considered as one of the important types of complementary and alternative medicine and receives increasing attention worldwide. The dried root of Polygonum cuspidatum Sieb. et Zucc. (also known as “Hu Zhang” in Chinese is one of the medicinal herbs listed in the Pharmacopoeia of the People's Republic of China. Hu Zhang is widely distributed in the world. It can be found in Asia and North America and is used as folk medicine in countries such as Japan and Korea. In China, Hu Zhang is usually used in combination with other TCM herbs. The therapeutic uses of those Hu Zhang-containing TCM prescriptions or formulations are for treating cough, hepatitis, jaundice, amenorrhea, leucorrhea, arthralgia, burns and snake bites. Recent pharmacological and clinical studies have indicated that Hu Zhang has antiviral, antimicrobial, anti-inflammatory, neuroprotective, and cardioprotective functions. This review gives a summary of the reported therapeutic effects of the active compounds and the different extracts of Hu Zhang.

  7. Teede REV-2 otsustas börsile minna / Gea Velthut-Sokka

    Index Scriptorium Estoniae

    Velthut-Sokka, Gea, 1977-

    2004-01-01

    Eelmise aasta edukaima ehitusfirma Teede REV-2 ja tema suurinvestori Alta Kapital esindajad põhjendavad börsile mineku otsust. Tabelid ja diagrammid: Veerandi aktsiate omanikuvahetus börsilemineku peaprooviks

  8. Rev. Konstanty Michalski's life and work. On his 50th anniversary of death

    Directory of Open Access Journals (Sweden)

    Stanisław Piech

    1998-12-01

    Full Text Available The article outlines the life and work of Rev. Konstanty Michalski, a lecturer at the Department of Theology at the Jagiellonian University, on the fiftieth anniversary of his death (deceased in 1947.

  9. Multiple roles of Rev3, the catalytic subunit of polzeta in maintaining genome stability in vertebrates

    NARCIS (Netherlands)

    E. Sonoda (Eiichiro); S. Takeda (Shiunichi); T. Okada (Takashi); G.Y. Zhao (Guang); S. Tateishi (Satoshi); K. Araki (Kasumi); M. Yamaizumi (Masaru); T. Yagi (Takashi); N.S. Verkaik (Nicole); D.C. van Gent (Dik); M. Takata (Minoru)

    2003-01-01

    textabstractTranslesion DNA synthesis (TLS) and homologous DNA recombination (HR) are two major postreplicational repair (PRR) pathways. The REV3 gene of Saccharomyces cerevisiae encodes the catalytic subunit of DNA polymerase zeta, which is involved in mutagenic TLS. To

  10. 78 FR 27971 - Determination That REV-EYES (Dapiprazole Hydrochloride Ophthalmic Solution), 0.5%, Was Not...

    Science.gov (United States)

    2013-05-13

    ... HUMAN SERVICES Food and Drug Administration Determination That REV-EYES (Dapiprazole Hydrochloride... determined that REV-EYES (dapiprazole hydrochloride ophthalmic solution), 0.5%, was not withdrawn from sale... refer to a listed drug. REV-EYES (dapiprazole hydrochloride ophthalmic solution), 0.5%, is the...

  11. 75 FR 27840 - NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-05-18

    ...: 2010-11842] NUCLEAR REGULATORY COMMISSION [NRC-2010-0080] NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3...: ``NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for General... the existing guidance contained in Supplement 3 to NUREG- 0654/FEMA-REP-1, Rev. 1, ``Criteria...

  12. 75 FR 10524 - NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-03-08

    ... COMMISSION NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for... document entitled: ``NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action...-4737, or by e-mail to pdr.resource@nrc.gov . NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3,...

  13. Comment on : "A novel approach for solving the Fisher equation using Exp-function method" [Phys. Lett. A 372 3836

    NARCIS (Netherlands)

    Kudryashov, Nikolai A.

    2009-01-01

    Using Exp-function method Ozis and Koroglu [T. Ozis, C. Koroglu, Phys. Lett. A 372 (2008) 3836] have found exact "solutions" of the Fisher equation. In this comment we demonstrate that all these solutions do not satisfy the Fisher equation. The efficiency of application of Exp-function method to sea

  14. Comment on "Soret motion in non-ionic binary molecular mixtures" [J. Chem. Phys. 135, 054102 (2011)].

    Science.gov (United States)

    Semenov, Semen N; Schimpf, Martin E

    2012-09-28

    The material transport equations derived in the article by Leroyer and Würger [J. Chem. Phys. 135, 054102 (2011)] do not adequately provide a description of material transport in liquid binary systems. An alternate approach based on non-equilibrium thermodynamics is presented.

  15. Comment on "Structure and properties of reduced LaCoO3" [J. Appl. Phys. 44, 5553 (1973)

    Science.gov (United States)

    Zhang, A. M.

    2013-06-01

    A hexagonal crystal lattice with the perovskite structure can be treated as rhombohedral or pseudocubic structure. The publication by Sis and Wirtz [J. Appl. Phys. 44, 5553 (1973)] presented a schematic diagram to explain these relationships, but made a mistake in the hexagonal lattice, which is corrected here.

  16. Multi-Faceted Post-Transcriptional Functions of HIV-1 Rev

    Directory of Open Access Journals (Sweden)

    Kuan-Teh Jeang

    2012-07-01

    Full Text Available Post-transcriptional regulation of HIV-1 gene expression is largely governed by the activities of the viral Rev protein. In this minireview, the multiple post-transcriptional activities of Rev in the export of partially spliced and unspliced HIV-1 RNAs from the nucleus to the cytoplasm, in the translation of HIV-1 transcripts, and in the packaging of viral genomic RNAs are reviewed in brief.

  17. Offshore wind farms in the local environment - an examination at Horns Rev Offshore Wind Farm. Background report; Havvindmoeller i lokalomraadet - en undersoegelse ved Horns Rev Havmoellepark. Baggrundsrapport

    Energy Technology Data Exchange (ETDEWEB)

    Kuehn, Susanne

    2005-07-01

    Horns Rev Offshore Wind Farm was built in 2002. A presentation is made of a sociological, qualitative survey on the local community's reception of the offshore wind farm. The survey aims at identifying attitudes towards the farm before and after the construction, with a view to identifying possible changes in attitudes, and explain the reasons for these (ml)

  18. Application of RevPASH index in restaurant management%RevPASH指标在餐饮管理中的应用

    Institute of Scientific and Technical Information of China (English)

    王琪

    2013-01-01

    随着社会经济水平的提高,我国的餐饮业发展非常迅速。餐饮业和其他企业一样,也需要做好管理,而RevPASH指标作为一种重要的管理方法已在餐饮业得到了广泛的应用。文章主要对RevPASH指标在餐饮管理中的应用做了探讨。%With the improvement of socio-economic level, catering industry in our country is developing very quickly. The catering industry and other enterprises, also need to do a good job in the management, and take RevPASH index as an important management method, has been widely used in the food and beverage industry. This paper explored the application of RevPASH index in restaurant management.

  19. Unperturbed posttranscriptional regulatory Rev protein function and HIV-1 replication in astrocytes.

    Directory of Open Access Journals (Sweden)

    Ashok Chauhan

    Full Text Available Astrocytes protect neurons, but also evoke proinflammatory responses to injury and viral infections, including HIV. There is a prevailing notion that HIV-1 Rev protein function in astrocytes is perturbed, leading to restricted viral replication. In earlier studies, our finding of restricted viral entry into astrocytes led us to investigate whether there are any intracellular restrictions, including crippled Rev function, in astrocytes. Despite barely detectable levels of DDX3 (Rev-supporting RNA helicase and TRBP (anti-PKR in primary astrocytes compared to astrocytic cells, Rev function was unperturbed in wild-type, but not DDX3-ablated astrocytes. As in permissive cells, after HIV-1 entry bypass in astrocytes, viral-encoded Tat and Rev proteins had robust regulatory activities, leading to efficient viral replication. Productive HIV-1 infection in astrocytes persisted for several weeks. Our findings on HIV-1 entry bypass in astrocytes demonstrated that the intracellular environment is conducive to viral replication and that Tat and Rev functions are unperturbed.

  20. CONSTRUCTION OF HU-PBL/SCID CHIMERAS AND DEVELOPMENT OF EBV-RELATED LYMPHOMAS

    Institute of Scientific and Technical Information of China (English)

    Run-liang Gan; Ke Lan; Zhi-hua Yin; Li-jiang Wang; Ying Song; Kai-tai Yao

    2005-01-01

    Objective To construct hu-PBL/SCID chimeras and to investigate the development of lymphoma and oncogenicity of the Epstein-Barr virus (EBV).Mtehods Human peripheral blood lymphocytes (PBLs) were isolated from healthy adult donors and transplanted intraperitoneally into severe combined immunodeficient (SCID) mice. Mice with hu-PBL engraftment from healthy EBV seronegative donors were injected intraperitoneally with EBV-containing supematant from suspension culture of B95-8 cell line (active infection), whereas mice receiving lymphocytes from healthy EBV seropositive donors were not re-infected with B95-8 derived EBV (latent infection). Pathological examination and molecular analysis were performed on experimental animals and induced neoplasms.Results In the early stage of this experiment, 12 mice died of acute graft-versus-host disease, mortality was 34.3%(12/35 mice) with an average life span of 17.5 days. In 19 survival hu-PBL/SCID chimeric recipients from 12 healthy donors,tumor incidence was 84.2% (16/19 mice). The average survival time of tumor-bearing mice was 65.5 days. EBV-related neoplasms in SCID mice were nodular tumors with aggressive and fatal features. Histological morphology of tumors exhibited diffuse large cell lymphomas. Immunohistochemistry revealed that LCA (CD45) and L26 (CD20) were positive, but both PS1 (CD3) and UCHL-1 (CD45RO) were negative, and EBV products ZEBRA, LMP1, and EBNA2 were expressed in a small number of tumor cells. EB virus particles were seen in the nuclei of some tumor cells by electron microscopy, and EBV DNA could be amplified in the tumor tissues by PCR. In situ hybridization indicated that the nuclei of tumor cells contained human-specific Alu sequence.Conclusions EBV-induced tumors were human B-cell malignant lymphomas. We obtained direct causative evidence dealing with EBV-associated tumor deriving from normal human cells.

  1. 'Hu Hong' (bad thing): parental perceptions of teenagers' sexuality in urban Vietnam.

    Science.gov (United States)

    Do, Lan Anh Thi; Boonmongkon, Pimpawun; Paek, Seung Chun; Guadamuz, Thomas E

    2017-02-28

    Teenagers under 18 years old in Vietnam are considered as minors who usually lack the autonomy to make decisions. They are also sometimes viewed as contributors to social evils including crime, violence and substance use. Moreover, most Vietnamese teenagers have unsafe sex before marriage. The objective of this study is to explore the parental perceptions relating to their teenagers' sexuality, particularly the social and cultural forces, that may hinder access to sexuality information. Guided by a Community Advisory Board (CAB), this qualitative study uses four focus group discussions (FGDs) consisting of 12 mothers and 12 fathers, as well as twelve individual in-depth interviews (IDIs) with a diverse sample of parents of teens in Ho Chi Minh City (HCMC), Vietnam. Content and discourse analysis were conducted, based on Foucauldian concepts. Four themes emerged: 1) Meanings of sexuality and sexuality education, 2) Early sexual intercourse destroys teenagers' future, 3) Teenagers are not hu hong (spoil/bad thing), are innocent and virgin, and 4) Policing and controlling of sexual intercourse among teens. Parents did not view their teenage children as sexual beings; those who are sexual are considered hu hong. Parents believed that teens need to be policed and controlled to prevent them from becoming hu hong, particularly girls. Controlling of sexuality information by parents was therefore common in HCMC, but differed by gender and educational levels of parents. For example, fathers more than mothers were not comfortable teaching their teenage children about sex and sexuality. Parents with higher education police their teenage children's usage of the Internet and social media, while parents with lower education control who can be friends with their teenage children. Vietnamese parents in general have negative views of sex and sexuality education for their teenage children. Recognizing that many Vietnamese teenagers have unsafe sex before marriage, parents need to

  2. Effects of weaning age on growth, nutrient digestibility and metabolism, and serum parameters in Hu lambs

    Directory of Open Access Journals (Sweden)

    Jianmin Chai

    2015-12-01

    Full Text Available This study was conducted to investigate the effect of weaning age on growth performance, nutrient digestion and metabolism, and serological indicators, and to obtain an optimal weaning age in Hu lambs. Forty-eight newborn Hu lambs (birth weight, 2.53 ± 0.14 kg were randomly divided into 4 groups. The lambs in control group (ER suckled their dams. The lambs in other three experimental groups were weaned on milk replacer at 10, 20, and 30 days of age (EW10, EW20, and EW30 groups, respectively. The results were as follows: 1 lambs in EW10 and EW30 groups had a lower (P  0.05 among groups; however, the apparent digestibility and deposition of calcium in early weaned lambs were lower (P < 0.05 than those in ewe-reared lambs. 4 The albumin content in EW30 group was lower (P < 0.05 than that in ER group; the globulin content in EW30 group was higher (P < 0.05 than that in other groups; the content of serum insulin-like growth factor-Ⅰ in weaned lambs tended to increase compared with lambs in ER group. Finally, the growth rate of lambs decreased within 10 days post-weaning, but early weaning boosted creep feed intake, leading to better growth and health later in life. The Hu lambs can be weaned on milk replacer and creep feed at 10 days of age.

  3. Chemical Constituents from the Seeds of Ziziphus jujuba var. Spinosa (Bunge) Hu

    Institute of Scientific and Technical Information of China (English)

    Li-Mei LI; Xun LIAO; Shu-Lin PENG; Li-Sheng DING

    2005-01-01

    To search for new and bioactive minor components from traditional Chinese medicines, a new compound, named jujuphenoside (1), was isolated from the seeds of Ziziphusjujuba var. spinosa (Bunge)Hu. The structure of jujuphenoside was elucidated by spectral and chemical methods, particularly twodimensional nuclear magnetic resonance analysis. Together with the new compound, 22 known compounds were also isolated and identified from the seeds of Z. jujuba var. spinosa, among which, epiceanothic acid (2) was first obtained from natural resources, whereas compounds 7-16 were first obtained from this plant.

  4. Opsoclonus-myoclonus and anti-Hu positive limbic encephalitis in a patient with neuroblastoma.

    Science.gov (United States)

    Morales La Madrid, Andres; Rubin, Charles M; Kohrman, Michael; Pytel, Peter; Cohn, Susan L

    2012-03-01

    Opsoclonus-myoclonus syndrome (OMS) is seen in 2-3% of children with neuroblastoma and is believed to be caused by an autoimmune process elicited by the tumor. Although long-term neurologic sequelae are common in children with OMS, limbic encephalitis has not previously been reported. We report a child who developed limbic encephalitis associated with anti-Hu antibodies, 6 years after her initial diagnosis of neuroblastoma and OMS. This case demonstrates that patients with neuroblastoma and OMS are at risk for developing new paraneoplastic symptoms years after their original diagnosis and emphasizes the need for careful long-term follow-up.

  5. Micología Lista de Huéspedes Colombianos

    Directory of Open Access Journals (Sweden)

    Obregon Botero Rafael

    1964-12-01

    Full Text Available El presente trabajo es una lista de hongos encontrados en Colombia. Está ordenada por Familias del huésped y cada nombre científico del hongo va seguido por la mención de los lugares donde fue encontrado así como la altura de tales localidades. Luego precede un número que corresponde a la colección existente en el Ministerio de Agricultura de Colombia. No pretendo sino iniciar la labor que han de completar especialistas mejor informados y espero que sea de alguna utilidad para los que se dedican a esta rama de las Ciencias Naturales.

  6. Hybrid Semiloof elements for plates and shells based upon a modified Hu-Washizu principle

    Science.gov (United States)

    Pian, T. H. H.; Sumihara, K.

    1984-01-01

    Hybrid SemiLoof elements for plates and shells are developed based upon modified Hu-Washizu principle. In the new version of the assumed stress hybrid formulation the equilibrium equations are satisfied through the introduction of internal displacement parameters as Lagrange multipliers. The inversion of the resulting H-matrices is simplified particularly when the stresses are expressed in terms of natural coordinates. A 24-DOF triangular element and a 32-DOF quadrilateral element based on shallow shell theory are derived and evaluated.

  7. HuLiS, a program to teach mesomerism and more

    Science.gov (United States)

    Carissan, Yannick; Goudard, Nicolas; Hagebaum-Reignier, Denis; Humbel, Stéphane

    2016-08-01

    The HuLiS program is presented as a tool to decompose a delocalized wave function as a linear combination of localized electronic structures. The principles of the energy based HL-CI and the overlap based schemes HL-P are developed. The results obtained for a set of 10 relevant organic molecules are shown to compare very well with high level quantum chemistry calculations. A trust factort is introduced and its use is shown on the allyl radical case where symmetry must be taken into account.

  8. MicroRNA-16 Modulates HuR Regulation of Cyclin E1 in Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Xun Guo

    2015-03-01

    Full Text Available RNA binding protein (RBPs and microRNAs (miRNAs or miRs are post-transcriptional regulators of gene expression that are implicated in development of cancers. Although their individual roles have been studied, the crosstalk between RBPs and miRNAs is under intense investigation. Here, we show that in breast cancer cells, cyclin E1 upregulation by the RBP HuR is through specific binding to regions in the cyclin E1 mRNA 3' untranslated region (3'UTR containing U-rich elements. Similarly, miR-16 represses cyclin E1, dependent on its cognate binding sites in the cyclin E1 3'UTR. Evidence in the literature indicates that HuR can regulate miRNA expression and recruit or dissociate RNA-induced silencing complexes (RISC. Despite this, miR-16 and HuR do not affect the other’s expression level or binding to the cyclin E1 3'UTR. While HuR overexpression partially blocks miR-16 repression of a reporter mRNA containing the cyclin E1 3'UTR, it does not block miR-16 repression of endogenous cyclin E1 mRNA. In contrast, miR-16 blocks HuR-mediated upregulation of cyclin E1. Overall our results suggest that miR-16 can override HuR upregulation of cyclin E1 without affecting HuR expression or association with the cyclin E1 mRNA.

  9. Direct and Indirect Suppression of Interleukin-6 Gene Expression in Murine Macrophages by Nuclear Orphan Receptor REV-ERBα

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    Shogo Sato

    2014-01-01

    Full Text Available It is now evident that many nuclear hormone receptors can modulate target gene expression. REV-ERBα, one of the nuclear hormone receptors with the capacity to alter clock function, is critically involved in lipid metabolism, adipogenesis, and the inflammatory response. Recent studies suggest that REV-ERBα plays a key role in the mediation between clockwork and inflammation. The purpose of the current study was to investigate the role of REV-ERBα in the regulation of interleukin-6 (il6 gene expression in murine macrophages. REV-ERBα agonists, or overexpression of rev-erbα in the murine macrophage cell line RAW264 cells, suppressed the induction of il6 mRNA following a lipopolysaccharide (LPS endotoxin challenge. Also, rev-erbα overexpression decreased LPS-stimulated nuclear factor κB (NFκB activation in RAW264 cells. We showed that REV-ERBα represses il6 expression not only indirectly through an NFκB binding motif but also directly through a REV-ERBα binding motif in the murine il6 promoter region. Furthermore, peritoneal macrophages from mice lacking rev-erbα increased il6 mRNA expression. These data suggest that REV-ERBα regulates the inflammatory response of macrophages through the suppression of il6 expression. REV-ERBα may therefore be identified as a potent anti-inflammatory receptor and be a therapeutic target receptor of inflammatory diseases.

  10. Nullbasic, a potent anti-HIV tat mutant, induces CRM1-dependent disruption of HIV rev trafficking.

    Directory of Open Access Journals (Sweden)

    Min-Hsuan Lin

    Full Text Available Nullbasic, a mutant of the HIV-1 Tat protein, has anti-HIV-1 activity through mechanisms that include inhibition of Rev function and redistribution of the HIV-1 Rev protein from the nucleolus to the nucleoplasm and cytoplasm. Here we investigate the mechanism of this effect for the first time, establishing that redistribution of Rev by Nullbasic is not due to direct interaction between the two proteins. Rather, Nullbasic affects subcellular localization of cellular proteins that regulate Rev trafficking. In particular, Nullbasic induced redistribution of exportin 1 (CRM1, nucleophosmin (B23 and nucleolin (C23 from the nucleolus to the nucleus when Rev was coexpressed, but never in its absence. Inhibition of the Rev:CRM1 interaction by leptomycin B or a non-interacting RevM10 mutant completely blocked redistribution of Rev by Nullbasic. Finally, Nullbasic did not inhibit importin β- or transportin 1-mediated nuclear import, suggesting that cytoplasmic accumulation of Rev was due to increased export by CRM1. Overall, our data support the conclusion that CRM1-dependent subcellular redistribution of Rev from the nucleolus by Nullbasic is not through general perturbation of either nuclear import or export. Rather, Nullbasic appears to interact with and disrupt specific components of a Rev trafficking complex required for its nucleocytoplasmic shuttling and, in particular, its nucleolar accumulation.

  11. 可在鱼体内表达hu-IFN-α的基因重组构建及转化%Construction of a Fish Expression hu-IFN-α Recombinant Gene

    Institute of Scientific and Technical Information of China (English)

    张学文; 章怀云; 符少辉; 陈韬; 陈立祥; 肖调义

    2001-01-01

    通过分子重组,将人α-干扰素(hu-IFN-α)基因编码序列克隆到鲤鱼β-肌动蛋白基因启动子下游,构建成能在鱼体内组成型表达hu-IFN-α的基因重组分子。经限制性酶切分析、Southern杂交和序列测定,证明重组分子构建的正确性。采用显微注射法将这一重组基因转化草鱼受精卵,获得了表达人α-干扰素的转基因草鱼。%The coding sequence of hu-IFN-α gene is cloned under the control of Carp β-actin gene promoter. Thus a fish constantly expression of hu-IFN-α gene recombinant is constructed. Southern blot and sequencing results show that the recombinant molecule is correctly constructed. The recombinant gene is transferred into fertilized zygotes of grass carp via microinjection. And hu-IFN-α is detected out in 20% of transgenic fish by ELISA.

  12. Impact of REV-ERB alpha gene polymorphisms on obesity phenotypes in adult and adolescent samples.

    Science.gov (United States)

    Goumidi, L; Grechez, A; Dumont, J; Cottel, D; Kafatos, A; Moreno, L A; Molnar, D; Moschonis, G; Gottrand, F; Huybrechts, I; Dallongeville, J; Amouyel, P; Delaunay, F; Meirhaeghe, A

    2013-05-01

    REV-ERBα has been shown to regulate adipogenesis and lipid metabolism as well as to link the circadian timing system to whole body metabolic homeostasis. We thus tested whether polymorphisms in REV-ERBα could be associated with metabolic phenotypes in human population samples. We analyzed the associations between 5 REV-ERBα polymorphisms and anthropometric (body weight, body mass index (BMI), waist and hip circumferences), biochemical (plasma lipid, glucose and insulin levels) and clinical (systolic and diastolic blood pressure) variables in three population-based studies (MONICA Lille n=1155 adults, MONA LISA Lille n=1170 adults and HELENA n=1155 adolescents). We assessed in vitro, the potential influence of one REV-ERBα polymorphism in transient transfection assays using two different cell lines. We observed significant and consistent associations between the T minor allele of the REV-ERBα rs2071427 polymorphism (located in intron 1) and higher BMI (mean allele effect=+0.33 kg m(-2)) in the MONICA Lille (P=0.02), MONA LISA (P=0.02) and HELENA (P=0.03) studies. The odds ratios for obesity associated with this allele were 1.67 (1.00-2.79) (P=0.05) in MONICA Lille, 1.29 (1.01-1.65) (P=0.04) in MONA LISA Lille and the odds ratio for overweight was 1.48 (1.08-2.03) (P=0.01) in HELENA. In transfection experiments in human hepatocyte-derived cell lines, the REV-ERBα intron 1 directed the transcription of a luciferase reporter gene independently of the rs2071427 polymorphism. Our results suggest that the REV-ERBα rs2071427 polymorphism modulates body fat mass in both adult and young people.

  13. REV-ERBalpha participates in circadian SREBP signaling and bile acid homeostasis.

    Directory of Open Access Journals (Sweden)

    Gwendal Le Martelot

    2009-09-01

    Full Text Available In mammals, many aspects of behavior and physiology, and in particular cellular metabolism, are coordinated by the circadian timing system. Molecular clocks are thought to rely on negative feedback loops in clock gene expression that engender oscillations in the accumulation of transcriptional regulatory proteins, such as the orphan receptor REV-ERBalpha. Circadian transcription factors then drive daily rhythms in the expression of clock-controlled output genes, for example genes encoding enzymes and regulators of cellular metabolism. To gain insight into clock output functions of REV-ERBalpha, we carried out genome-wide transcriptome profiling experiments with liver RNA from wild-type mice, Rev-erbalpha knock-out mice, or REV-ERBalpha overexpressing mice. On the basis of these genetic loss- and gain-of-function experiments, we concluded that REV-ERBalpha participates in the circadian modulation of sterol regulatory element-binding protein (SREBP activity, and thereby in the daily expression of SREBP target genes involved in cholesterol and lipid metabolism. This control is exerted via the cyclic transcription of Insig2, encoding a trans-membrane protein that sequesters SREBP proteins to the endoplasmic reticulum membranes and thereby interferes with the proteolytic activation of SREBPs in Golgi membranes. REV-ERBalpha also participates in the cyclic expression of cholesterol-7alpha-hydroxylase (CYP7A1, the rate-limiting enzyme in converting cholesterol to bile acids. Our findings suggest that this control acts via the stimulation of LXR nuclear receptors by cyclically produced oxysterols. In conclusion, our study suggests that rhythmic cholesterol and bile acid metabolism is not just driven by alternating feeding-fasting cycles, but also by REV-ERBalpha, a component of the circadian clockwork circuitry.

  14. Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export function

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    St-Laurent Georges

    2006-11-01

    Full Text Available Abstract Background The HIV Rev protein is known to facilitate export of incompletely spliced and unspliced viral transcripts to the cytoplasm, a necessary step in virus life cycle. The Rev-mediated nucleo-cytoplasmic transport of nascent viral transcripts, dependents on interaction of Rev with the RRE RNA structural element present in the target RNAs. The C-terminal variant of dsRNA-binding nuclear protein 90 (NF90ctv has been shown to markedly attenuate viral replication in stably transduced HIV-1 target cell line. Here we examined a mechanism of interference of viral life cycle involving Rev-NF90ctv interaction. Results Since Rev:RRE complex formations depend on protein:RNA and protein:protein interactions, we investigated whether the expression of NF90ctv might interfere with Rev-mediated export of RRE-containing transcripts. When HeLa cells expressed both NF90ctv and Rev protein, we observed that NF90ctv inhibited the Rev-mediated RNA transport. In particular, three regions of NF90ctv protein are involved in blocking Rev function. Moreover, interaction of NF90ctv with the RRE RNA resulted in the expression of a reporter protein coding sequences linked to the RRE structure. Moreover, Rev influenced the subcellular localization of NF90ctv, and this process is leptomycin B sensitive. Conclusion The dsRNA binding protein, NF90ctv competes with HIV Rev function at two levels, by competitive protein:protein interaction involving Rev binding to specific domains of NF90ctv, as well as by its binding to the RRE-RNA structure. Our results are consistent with a model of Rev-mediated HIV-1 RNA export that envisions Rev-multimerization, a process interrupted by NF90ctv.

  15. Genetic determination of the enhanced drought resistance of rice maintainer HuHan2B by pedigree breeding.

    Science.gov (United States)

    Wei, Haibin; Feng, Fangjun; Lou, Qiaojun; Xia, Hui; Ma, Xiaosong; Liu, Yunhua; Xu, Kai; Yu, Xinqiao; Mei, Hanwei; Luo, Lijun

    2016-11-17

    The ongoing deficit of fresh water resource in rice growing regions has made the selection of water-saving and drought-resistance rice (WDR) a crucial factor in developing sustainable cultivation. HuHan2B, a new japonica maintainer for WDR breeding, had the same yield potential as recurrent parent HanFengB but showed improved drought resistance in fields. We investigated the genomic content accumulation and candidate genes passed from parent to offspring using the genomic and transcriptomic approaches. The genomic constitution indicated that the genetic similarity was 84% between HuHan2B and HanFengB; additionally, 7,256 genes with specific alleles were inherited by HuHan2B from parents other than HanFengB. The differentially expressed genes (DEGs) under drought stress showed that biological function was significantly enriched for transcript regulation in HuHan2B, while the oxidation-reduction process was primarily enriched in HanFengB. Furthermore, 36 DEGs with specific inherited alleles in HuHan2B were almost involved in the regulatory network of TFs and target genes. These findings suggested that major-effect genes were congregated and transformed into offspring in manner of interacting network by breeding. Thus, exploiting the potential biological function of allelic-influencing DEGs would be of great importance for improving selection efficiency and the overall genetic gain of multiple complex traits.

  16. HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes

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    Apostolia Guialis

    2013-10-01

    Full Text Available Post-transcriptional regulatory networks are dependent on the interplay of many RNA-binding proteins having a major role in mRNA processing events in mammals. We have been interested in the concerted action of the two RNA-binding proteins hnRNP A1 and HuR, both stable components of immunoselected hnRNP complexes and having a major nuclear localization. Specifically, we present here the application of the RNA-immunoprecipitation (RIP-Chip technology to identify a population of nuclear transcripts associated with hnRNP A1-RNPs as isolated from the nuclear extract of either HuR WT or HuR-depleted (KO mouse embryonic fibroblast (MEF cells. The outcome of this analysis was a list of target genes regulated via HuR for their association (either increased or reduced with the nuclear hnRNP A1-RNP complexes. Real time PCR analysis was applied to validate a selected number of nuclear mRNA transcripts, as well as to identify pre-spliced transcripts (in addition to their mature mRNA counterpart within the isolated nuclear hnRNP A1-RNPs. The differentially enriched mRNAs were found to belong to GO categories relevant to biological processes anticipated for hnRNP A1 and HuR (such as transport, transcription, translation, apoptosis and cell cycle indicating their concerted function in mRNA metabolism.

  17. Effects of rHu-EPO on Myocyte Apoptosis and Cardiac Function Follow-ing Acute Myocardial Infarction in Rats

    Institute of Scientific and Technical Information of China (English)

    YE Liang; DU Xinling; XIA Jiahong; JIANG Ping

    2005-01-01

    The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infarction in rats were studied. Thirty-two rats were divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI+ EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery. rHu-EPO was administered i. p. in MI+EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI+EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2, bax) and TUNEL dyeing. The results showed that hemodynamic function in MI+ EPO group was much better than in MI group.The number of the cells positive for bax and TUNEL in MI+EPO group was less than that in MI group. The number of the cells positive for bcl-2 in MI+EPO group was more than that in MI group. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function.

  18. The Use of Soluble Transferrin Receptor in the Detection of rHuEPO Abuse in Sports

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    Lorenzo Gordon

    2010-02-01

    Full Text Available Erythropoietin (EPO increases the number of circulating erythrocytes and muscle oxygenation. The recombinant forms of EPO have indiscriminately been used by athletes, mainly in endurance sports to increase their erythrocytes concentration, thus generating a better delivery of oxygen to the muscle tissue. The administration of recombinant human erythropoietin (rHuEPO except for therapeutic use was prohibited by the International Olympic Committee (IOC and its unauthorized use considered as doping. In the last few years, a number of studies using parameters indicative of accelerated erythropoiesis have investigated a number of indirect methods for the detection of rHuEPO abuse. No single indirect marker has been found that can satisfactorily demonstrated rHuEPO misuse. Soluble transferrin receptor (sTfR is a new marker of iron status and erythropoietic activity. It has been included in multivariable blood testing models for the detection of performance enhancing EPO abuse in sports. Indirect markers of altered erythropoiesis give reliable evidence of current or discontinued rHuEPO usage. This review describes the physical, biological and pharmacokinetic properties of endogenous EPO and its recombinant form. It also discusses the available strategies for the detection of rHuEPO abuse in sports, involving the use of sTfR concentration directly or in mathematical multivariate models.

  19. Comments on ``Lightning return stroke. A numerical calculation of the optical radiation'' [Phys. Fluids 29, 2736 (1986)

    Science.gov (United States)

    Hill, R. D.

    1987-08-01

    It is noted that the energy computed by Paxton et al. [Phys. Fluids 29, 2736 (1986)] for the dissipation in unit length of a typical lightning channel is much less than a widely quoted experimental value, and also less than an earlier computed value. A further comment on the work of Paxton et al. that a large fraction of energy is ``radiated away,'' is also made.

  20. Potential Risk of Hybridization in Ex Situ Collections of Two Endangered Species of Sinojackia Hu (Styracaceae)

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Spontaneous hybridization in ex situ facilities can undermine the genetic integrity of ex situ collections and potentially contaminate open-pollinated seeds or seedlings destined for the reintroduction of endangered plant species into the wild. In the present study, the potential risk of hybridization between two endangered Chinese endemic species, namely Sinojackia xylocarpa Hu and S. rehderiana Hu, which are naturally allopatric species but were conserved ex situ in Wuhan Botanical Garden (WBG), Wuhan, China, were investigated over three consecutive years from 2003 to 2005. The entire overlapping flowering period of the two species was 14-20 d and the two species shared the same pollinator insects during the entire flowering season in WBG. The floral isolation between the two species was not an issue in the ex situ collection at WBG. The results suggest an opportunity for pollen transfer between species and a potential risk of genetic introgression and loss of genetic identity of open-pollinated seeds produced in the ex situ collection of these two endangered species. An artificial reciprocal cross between S. xylocarpa and S. rehderiana confirmed that the two congener species could readily set seeds, indicating no post-pollination barriers to hybridization and the importance of spatial isolation as a barrier to inter-specific crossing. Therefore, to manage these crossable species with overlapping flowering times and shared pollination vectors in ex situ facilities, spatial isolation should be carefully considered to minimize the possibility of spontaneous hybridization.

  1. Combining wavelets transform and Hu moments with self-organizing maps for medical image categorization

    Science.gov (United States)

    Silva, Leandro A.; Del-Moral-Hernandez, Emilio; Moreno, Ramon A.; Furuie, Sérgio S.

    2011-10-01

    Images are fundamental sources of information in modern medicine. The images stored in a database and divided in categories are an important step for image retrieval. For an automatic categorization process, detailed analysis is done regarding image representation and generalization method. The baseline method for this process, in the medical image context, is using thumbnails and K-nearest neighbor (KNN), which is easily implemented and has had satisfactory results in literature. This work addresses an alternative method for automatic categorization, which jointly uses discrete wavelet transform with Hu's moments for image representation and self-organizing maps (SOM) neural networks combined with the KNN classifier (SOM-KNN), for medical image categorization. Furthermore, extensive experiments are conducted, to define the best wavelet family and to select the best coefficients set, to consider the remaining wavelet coefficients set (not selected as the best ones) through their Hu's moments, and to carry out a contrastive study with other successful approaches for categorization. The categorization result from a database with 10,000 images in 116 categories yielded 81.8% of correct rate, which is much better than the 67.9% obtained by the baseline method; and the time consumed in classification processing with SOM-KNN is 100 times shorter than KNN.

  2. Hu1-2: a metal-poor bipolar planetary nebula with fast collimated outflows

    CERN Document Server

    Fang, Xuan; Miranda, Luis F; Riera, Angels; Velazquez, Pablo F; Raga, Alejandro C

    2015-01-01

    We present narrow-band optical and near-IR imaging and optical long-slit spectroscopic observations of Hu1-2, a Galactic planetary nebula (PN) with a pair of [N II]-bright, fast-moving (> 340 km/s) bipolar knots. Intermediate-dispersion spectra are used to derive physical conditions and abundances across the nebula, and high-dispersion spectra to study the spatio-kinematical structure. Generally Hu1-2 has high He/H (~0.14) and N/O ratios (~0.9), typical of Type I PNe. On the other hand, its abundances of O, Ne, S, and Ar are low as compared with the average abundances of Galactic bulge and disc PNe. The position-velocity maps can be generally described as an hour-glass shaped nebula with bipolar expansion, although the morphology and kinematics of the innermost regions cannot be satisfactorily explained with a simple, tilted equatorial torus. The spatio-kinematical study confines the inclination angle of its major axis to be within 10 degrees of the plane of sky. As in the irradiated bow-shocks of IC4634 and ...

  3. Revisiting the proposed planetary system orbiting the eclipsing polar HU Aquarii

    CERN Document Server

    Wittenmyer, Robert A; Marshall, J P; Butters, O W; Tinney, C G

    2011-01-01

    It has recently been proposed, on the basis of eclipse-timing data, that the eclipsing polar cataclysmic variable HU Aquarii is host to at least two giant planets. However, that result has been called into question based upon the dynamical stability of the proposed planets. In this work, we present a detailed re-analysis of all eclipse timing data available for the HU Aquarii system, making use of standard techniques used to fit orbits to radial-velocity data. We find that the eclipse timings can be used to obtain a two-planet solution that does not require the presence of additional bodies within the system. We then perform a highly detailed dynamical analysis of the proposed planetary system. We show that the improved orbital parameters we have derived correspond to planets that are dynamically unstable on unfeasibly short timescales (of order 10^4 years or less). Given these results, we discuss briefly how the observed signal might in fact be the result of the intrinsic properties of the eclipsing polar, r...

  4. ReHuNa- La Red por la Humanización del Parto y Nacimiento

    Directory of Open Access Journals (Sweden)

    Daphne RATTNER

    2010-12-01

    Full Text Available La ReHuNa - Red por la Humanización del Parto y Nacimiento - es una organización de la sociedad civil que actúa desde 1993 en forma de red de asociados en el Brasil. Su principal objetivo es la divulgación de la asistencia y cuidados perinatales con base en evidencias científicas y de acuerdo a las recomendaciones de la Organización Mundial de la Salud. Esta red tuvo un papel fundamental en la estructura de un movimiento que hoy se denomina “humanización de la asistencia al parto y nacimiento”, que pretende disminuir las intervenciones innecesarias y promover cuidados al proceso del embarazo-parto-nacimiento-lactancia comprendido como un proceso natural y fisiológico basado en conocimientos científicos. Este artículo relata la historia de la ReHuNa y sus principales realizaciones y señala sus caminos futuros.

  5. The impact of the vaccination program for hemorrhagic fever with renal syndrome in Hu County, China.

    Science.gov (United States)

    Xiao, Dan; Wu, Kejian; Tan, Xin; Yan, Tiecheng; Li, Haitao; Yan, Yongping

    2014-02-03

    The incidence of hemorrhagic fever with renal syndrome (HFRS) in Hu County ranked third of all counties in China in 2010. Although this county has provided a HFRS vaccination program freely since 1994, the impact of HFRS remains quite substantial. In order to continue the vaccination program effectively and control HFRS, a detailed understanding of the effect of the vaccination program should be undertaken. The Cochran-Armitage trend test was employed to examine the temporal trends of HFRS incidences, mortality rate and vaccination compliance. Temporal cluster analysis was performed to detect time periods of high HFRS risk. Cross correlation analysis was conducted to detect the correlation between HFRS incidence and vaccination compliance. Wavelet analysis was employed to detect the shift of the periodicity of HFRS. Between 1971 and 2011, the HFRS incidence and mortality rate ranged from 9.53/100,000 to 300.57/100,000 and 0 to 24.91/100,000, respectively, with a fluctuating but distinctly declining trend (incidence: Z=-34.38, P1994. In conclusion, the increase in vaccination compliance may play an important role in HFRS control and prevention in Hu County, China.

  6. The SCID-hu mouse as a model for HIV-1 infection.

    Science.gov (United States)

    Aldrovandi, G M; Feuer, G; Gao, L; Jamieson, B; Kristeva, M; Chen, I S; Zack, J A

    1993-06-24

    During normal fetal ontogeny, one of the first organs to harbour CD4-positive cells is the thymus. This organ could therefore be one of the earliest targets infected by human immunodeficiency virus type 1 (HIV-1) in utero. HIV-1-infected cells and pathological abnormalities of the thymus have been seen in HIV-1-infected adults and children, and in some fetuses aborted from infected women. Studies of HIV-1 pathogenesis have been hampered by lack of a suitable animal model system. Here we use the SCID-hu mouse as a model to investigate the effect of virus infection on human tissue. The mouse is homozygous for the severe combined immunodeficiency (SCID) defect. The model is constructed by implanting human fetal liver and thymus under the mouse kidney capsule. A conjoint human organ develops, which allows normal maturation of human thymocytes. After direct inoculation of HIV-1 into these implants, we observed severe depletion of human CD4-bearing cells within a few weeks of infection. This correlated with increasing virus load in the implants. Thus the SCID-hu mouse may be a useful in vivo system for the study of HIV-1-induced pathology.

  7. RNA-binding protein HuD reduces triglyceride production in pancreatic β cells by enhancing the expression of insulin-induced gene 1.

    Science.gov (United States)

    Kim, Chongtae; Lee, Heejin; Kang, Hoin; Shin, Jung Jae; Tak, Hyosun; Kim, Wook; Gorospe, Myriam; Lee, Eun Kyung

    2016-04-01

    Although triglyceride (TG) accumulation in the pancreas leads to β-cell dysfunction and raises the chance to develop metabolic disorders such as type 2 diabetes (T2DM), the molecular mechanisms whereby intracellular TG levels are regulated in pancreatic β cells have not been fully elucidated. Here, we present evidence that the RNA-binding protein HuD regulates TG production in pancreatic β cells. Mouse insulinoma βTC6 cells stably expressing a small hairpin RNA targeting HuD (shHuD) (βTC6-shHuD) contained higher TG levels compared to control cells. Moreover, downregulation of HuD resulted in a decrease in insulin-induced gene 1 (INSIG1) levels but not in the levels of sterol regulatory element-binding protein 1c (SREBP1c), a key transcription factor for lipid production. We identified Insig1 mRNA as a direct target of HuD by using ribonucleoprotein immunoprecipitation (RIP) and biotin pulldown analyses. By associating with the 3'-untranslated region (3'UTR) of Insig1 mRNA, HuD promoted INSIG1 translation; accordingly, HuD downregulation reduced while ectopic HuD expression increased INSIG1 levels. We further observed that HuD downregulation facilitated the nuclear localization of SREBP1c, thereby increasing the transcriptional activity of SREBP1c and the expression of target genes involved in lipogenesis; likewise, we observed lower INSIG1 levels in the pancreatic islets of HuD-null mice. Taken together, our results indicate that HuD functions as a novel repressor of lipid synthesis in pancreatic β cells.

  8. cdma2000 1x EV-DO Rev.A的多流分组应用%MFPA of cdma2000 1x EV-DO Rev.A

    Institute of Scientific and Technical Information of China (English)

    摩托罗拉公司

    2007-01-01

    cdma2000 1x EV-DO版本A(即release A)将是中国CDMA运营商的3G选择.为使用户能同时支持不同QoS的实时应用,EV-DO Rev.A的数据平面引入了多流分组应用.为配合MFPA的应用,分组数据业务节点(PDSN)亦须做相应的升级.

  9. HuR-targeted nanotherapy in combination with AMD3100 suppresses CXCR4 expression, cell growth, migration, and invasion in lung cancer

    Science.gov (United States)

    Muralidharan, Ranganayaki; Panneerselvam, Janani; Chen, Allshine; Zhao, Yan Daniel; Munshi, Anupama; Ramesh, Rajagopal

    2015-01-01

    The CXCR4 chemokine receptor plays an important role in cancer cell metastasis. The CXCR4 antagonist, AMD3100, has limited efficacy in controlling metastasis. HuR, an RNA-binding protein, regulates CXCR4 in cancer cells. We therefore investigated whether targeting HuR using a siRNA-based nanoparticle plus AMD3100 would suppress CXCR4 and inhibit lung cancer metastasis. We treated human H1299 lung cancer cell with HuR-specific siRNA contained in a folate-targeted lipid nanoparticle (HuR-FNP) plus AMD3100, and compared this with AMD3100 alone, HuR-FNP alone and no treatment. HuR-FNP plus AMD3100 treatment produced a G1 phase cell-cycle arrest and reduced cell viability above and beyond the effects of AMD3100 alone. HuR and CXCR4 mRNA and protein expression levels were markedly reduced in all treatment groups. Phosphorylated (p) AKTS473 protein was also reduced. P27 protein expression increased with HuR-FNP and combination treatment. Promoter-based reporter studies showed that the combination inhibited CXCR4 promoter activity more than did either treatment alone. Cell migration and invasion was significantly reduced with all treatment; the combination provided the most inhibition. Reduced matrix metalloprotease (MMP) -2 and -9 expression was associated with reduced invasion in all treatment groups. Thus, we found that combined HuR and CXCR4 targeting effectively controlled lung cancer metastasis. PMID:26494555

  10. HuR-targeted nanotherapy in combination with AMD3100 suppresses CXCR4 expression, cell growth, migration and invasion in lung cancer.

    Science.gov (United States)

    Muralidharan, R; Panneerselvam, J; Chen, A; Zhao, Y D; Munshi, A; Ramesh, R

    2015-12-01

    The CXCR4 chemokine receptor has an important role in cancer cell metastasis. The CXCR4 antagonist, AMD3100, has limited efficacy in controlling metastasis. HuR, an RNA-binding protein, regulates CXCR4 in cancer cells. We therefore investigated whether targeting HuR using a siRNA-based nanoparticle plus AMD3100 would suppress CXCR4 and inhibit lung cancer metastasis. We treated human H1299 lung cancer cells with HuR-specific siRNA contained in a folate-targeted lipid nanoparticle (HuR-FNP) plus AMD3100, and compared this with AMD3100 alone, HuR-FNP alone and no treatment. HuR-FNP plus AMD3100 treatment produced a G1 phase cell cycle arrest and reduced cell viability above and beyond the effects of AMD3100 alone. HuR and CXCR4 mRNA and protein expression levels were markedly reduced in all treatment groups. Phosphorylated (p) AKT(S473) protein was also reduced. P27 protein expression increased with HuR-FNP and combination treatment. Promoter-based reporter studies showed that the combination inhibited CXCR4 promoter activity more than did either treatment alone. Cell migration and invasion was significantly reduced with all treatments; the combination provided the most inhibition. Reduced matrix metalloprotease (MMP)-2 and -9 expression was associated with reduced invasion in all treatment groups. Thus, we found that combined HuR and CXCR4 targeting effectively controlled lung cancer metastasis.

  11. Expression of ARE-binding proteins AUF1 and HuR in follicular adenoma and carcinoma of thyroid gland.

    Science.gov (United States)

    Trojanowicz, B; Sekulla, C; Dralle, H; Hoang-Vu, C

    2016-01-01

    Both adenylate-uridylate rich elements binding proteins AUF1 and HuR may participate in thyroid carcinoma progression. In this study we investigated the expression of both factors on a protein level with a special focus on follicular adenoma and follicular thyroid carcinoma. By employment of immunofluorescence and western blot on 68 thyroid tissues including 7 goiter, 16 follicular adenoma (4 adenomatous hyperplasia), 19 follicular thyroid carcinomas, 13 papillary thyroid carcinomas and 14 undifferentiated thyroid carcinomas we investigated protein expression of AUF1 and HuR. In addition to previous results we demonstrated that AUF1 and HuR are significantly up-regulated in carcinoma tissues as compared with follicular adenoma or goiter tissues. Furthermore, by evaluation of AUF1 or HuR expression, or combination of both proteins on total tissue lysates, we were able to demonstrate a significant difference between follicular adenoma and follicular thyroid carcinoma. Overexpression of AUF1 and HuR is a common finding observed in thyroid malignancy. Analysis of the tissues obtained by surgical resection as demonstrated in this study is comparable to a fine needle aspiration and in combination with AUF1/HuR immuno-analysis may support the conventional immunohistological investigations. The promising results of this study were performed on relatively small collective, but justify future development of a quick thyroid diagnostic test on larger cohort of the patients, especially for thyroid samples which are inadequate for histological examinations.

  12. Empirical investigation of wind farm blockage effects in Horn Rev 1 offshore wind farm

    DEFF Research Database (Denmark)

    Mitraszewski, Karol; Hansen, Kurt Schaldemose; Nygaard, Nicolai;

    We present an empirical study of wind farm blockage effects based on Horns Rev 1 SCADA data. The mean inflow non-uniformities in wind speed are analyzed by calculating the mean power outputs of turbines located along the outer edges of the farm for different wind directions, wind speeds and stabi......We present an empirical study of wind farm blockage effects based on Horns Rev 1 SCADA data. The mean inflow non-uniformities in wind speed are analyzed by calculating the mean power outputs of turbines located along the outer edges of the farm for different wind directions, wind speeds...

  13. Administrative Circular No. 13 (Rev. 4) - Guarantees for representatives of the personnel

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 13 (Rev. 4) entitled "Guarantees for representatives of the personnel", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 22 March 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208527.   This revised circular cancels and replaces Administrative Circular No. 13 (Rev. 3) also entitled "Guarantees for representatives of the personnel" of January 2014. This document contains a single change to reflect the terminology under the new career structure: the term "career path" is replaced by "grade". This circular will enter into force on 1st September 2016.

  14. Unprecedented NES non-antagonistic inhibitor for nuclear export of Rev from Sida cordifolia.

    Science.gov (United States)

    Tamura, Satoru; Kaneko, Masafumi; Shiomi, Atsushi; Yang, Guang-Ming; Yamaura, Toshiaki; Murakami, Nobutoshi

    2010-03-15

    Bioassay-guided separation from the MeOH extract of the South American medicinal plant Sida cordifolia resulted in isolation of (10E,12Z)-9-hydroxyoctadeca-10,12-dienoic acid (1) as an unprecedented NES non-antagonistic inhibitor for nuclear export of Rev. This mechanism of action was established by competitive experiment by the biotinylated probe derived from leptomycin B, the known NES antagonistic inhibitor. Additionally, structure-activity relationship analysis by use of the synthesized analogs clarified cooperation of several functionalities in the Rev-export inhibitory activity of 1. Copyright 2010 Elsevier Ltd. All rights reserved.

  15. Switching studies for the Horns Rev 2 wind farm main cable

    DEFF Research Database (Denmark)

    Jensen, Christian Flytkjær; Faria da Silva, Filipe; Bak, Claus Leth

    2011-01-01

    This article proposes a method of constructing a PSCAD model suitable for switching studies in a system containing long HVAC cables. The transmission network connection to the 215 MW offshore wind farm Horns Rev 2 is used as a case study. The connection to Horns Rev 2 consists of two land cable...... model used in PSCAD are given. Results obtained using the model are compared to full scale measurement of the energization of the system, and good agreement is found. The influence of different simulation parameters are examined; amongst them the modeling of the screen, the relative permittivity...

  16. Rev-erbα and the circadian transcriptional regulation of metabolism

    DEFF Research Database (Denmark)

    Gerhart-Hines, Z.; Lazar, M. A.

    2015-01-01

    The circadian clock orchestrates the coordinated rhythmicity of numerous metabolic pathways to anticipate daily and seasonal changes in energy demand. This vital physiol. function is controlled by a set of individual clock components that are present in each cell of the body, and regulate each...... other as well as clock output genes. A key factor is the nuclear receptor, Rev-erbα, a transcriptional repressor which functions not only as a clock component but also as a modulator of metabolic programming in an array of tissues. This review explores the role of Rev-erbα in mediating this crosstalk...

  17. Administrative Circular No. 11 (Rev. 4) - Categories of members of the personnel

    CERN Multimedia

    HR Department

    2016-01-01

    Administrative Circular No. 11 (Rev. 4) entitled "Categories of members of the personnel", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 29 April 2016, will be available on 1 August 2016 via this following link.    This revised circular cancels and replaces the Administrative Circular No. 11 (Rev. 3) also entitled "Categories of members of the personnel" of September 2014. The main changes concern the status of apprentices and their transfer from the category of employed members of personnel to associated members of personnel. This circular will enter into force on 1 August 2016. Department Head Office

  18. Investigations of migratory birds during operation of Horns Rev offshore wind farm. Annual status report 2004

    Energy Technology Data Exchange (ETDEWEB)

    Kjaer Christensen, T.; Hounisen, J.P. [NERI, Dept. of Wildlife Ecology and Biodiversity, Roskilde (Denmark)

    2005-07-01

    The aim of the project is to assess the collision risk between birds and wind turbines at the Horns Rev wind farm. The study focused on describing bird movements in relation to the wind farm and to identify the species-specific behavioural responses towards the wind turbines shown by migrating and staging bird species. The study was based on data from spring 2004. The Horns Rev area lies in a region known to be of importance for substantial water bird migration as well as holding internationally important numbers of several wintering and staging water bird species. (au)

  19. Administrative Circular No. 22B (Rev. 2) - Compensation for hours of long-term shift work

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 22B (Rev. 2) entitled "Compensation for hours of long-term shift work",  approved by the Director-General following discussion in the Standing Concertation Committee meeting on 22 March 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208538.   This revised circular cancels and replaces Administrative Circular No. 22B (Rev. 1) also entitled "Compensation for hours of long-term shift work" of March 2011. This document contains minor changes to reflect the new career structure. This circular will enter into force on 1st September 2016.

  20. Antonio Rodríguez Huéscar y José Ortega y Gasset: Tres cartas

    OpenAIRE

    Esteban Enguita, José Emilio; Lasaga Medina, José

    2015-01-01

    El objetivo principal de este artículo es comentar las cartas que se enviaron Antonio Rodríguez Huéscar y José Ortega y Gasset entre 1942 y 1943. Dichas cartas se incluyen en el artículo. The principal aim of this paper is to comment on the letters which Antonio Rodríguez Huéscar and José Ortega y Gasset sent each other between 1942 and 1943. The above said letters are included in the paper

  1. The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA

    OpenAIRE

    Lopez de Silanes, I.; Gorospe, M.; Taniguchi, H; Abdelmohsen, K; Srikantan, S.; Alaminos, M.; Berdasco, M.; Urdinguio, R. G.; Fraga, M. F.; Jacinto, F. V.; Esteller, M.

    2009-01-01

    The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression levels increase during tumorigenesis. Here, we present evidence that the expression of DNMT3b is post-transcriptionally regulated by HuR, an RNA-binding protein that stabilizes and/or modulates the translation of target mRNAs. The presence of a putative HuR-recognition motif in the DNMT3b 3???UTR pr...

  2. Comparison of the Debye–Hückel and the Mean Spherical Approximation Theories for Electrolyte Solutions

    DEFF Research Database (Denmark)

    Maribo-Mogensen, Bjørn; Kontogeorgis, Georgios M.; Thomsen, Kaj

    2012-01-01

    The thermodynamics of electrolyte solutions has been investigated by many scientists throughout the last century. While several theories have been presented, the most popular models for the electrostatic interactions are based on the Debye–Hückel and mean spherical approximation (MSA) theories....... In this paper we investigate the differences between the Debye–Hückel and the MSA theories, and comparisons of the numerical results for the Helmholtz energy and its derivatives with respect to temperature, volume and composition are presented. The investigation shows that the nonrestricted primitive MSA...

  3. Anti-solar differential rotation on the active sub-giant HU Virginis

    Science.gov (United States)

    Harutyunyan, G.; Strassmeier, K. G.; Künstler, A.; Carroll, T. A.; Weber, M.

    2016-08-01

    Context. Measuring surface differential rotation (DR) on different types of stars is important when characterizing the underlying stellar dynamo. It has been suggested that anti-solar DR laws can occur when strong meridional flows exist. Aims: We aim to investigate the differential surface rotation on the primary star of the RS CVn binary, HU Vir, by tracking its starspot distribution as a function of time. We also aim to recompute and update the values for several system parameters of the triple system HU Vir (close and wide orbits). Methods: Time-series high-resolution spectroscopy for four continuous months was obtained with the 1.2-m robotic STELLA telescope. Nine consecutive Doppler images were reconstructed from these data, using our line-profile inversion code iMap. An image cross-correlation method was applied to derive the surface differential-rotation law for HU Vir. New orbital elements for the close and the wide orbits were computed using our new STELLA radial velocities (RVs) combined with the RV data available in the literature. Photometric observations were performed with the Amadeus Automatic Photoelectric Telescope (APT), providing contemporaneous Johnson-Cousins V and I data for approximately 20 yrs. This data was used to determine the stellar rotation period and the active longitudes. Results: We confirm anti-solar DR with a surface shear parameter α of -0.029 ± 0.005 and -0.026 ± 0.009, using single-term and double-term differential rotation laws, respectively. These values are in good agreement with previously claimed results. The best fit is achieved assuming a solar-like double-term law with a lap time of ≈400 d. Our orbital solutions result in a period of 10.387678 ± 0.000003 days for the close orbit and 2726 ± 7 d (≈7.5 yr) for the wide orbit. A Lomb-Scarge (L-S) periodogram of the pre-whitened V-band data reveals a strong single peak providing a rotation period of 10.391 ± 0.008 d, well synchronized to the short orbit. Based on

  4. Isolation and characterization of a catalase gene "HuCAT3" from pitaya (Hylocereus undatus) and its expression under abiotic stress.

    Science.gov (United States)

    Nie, Qiong; Gao, Guo-Li; Fan, Qing-jie; Qiao, Guang; Wen, Xiao-Peng; Liu, Tao; Peng, Zhi-Jun; Cai, Yong-Qiang

    2015-05-25

    Abiotic stresses usually cause H2O2 accumulation, with harmful effects, in plants. Catalase may play a key protective role in plant cells by detoxifying this excess H2O2. Pitaya (Hylocereus undatus) shows broad ecological adaptation due to its high tolerance to abiotic stresses, e.g. drought, heat and poor soil. However, involvement of the pitaya catalase gene (HuCAT) in tolerance to abiotic stresses is unknown. In the present study, a full-length HuCAT3 cDNA (1870 bp) was isolated from pitaya based on our previous microarray data and RACE method. The cDNA sequence and deduced amino acid sequence shared 73-77% and 75-80% identity with other plant catalases, respectively. HuCAT3 contains conserved catalase family domain and catalytic sites. Pairwise comparison and phylogenetic analysis indicated that HuCAT3 is most similar to Eriobotrya japonica CAT, followed by Dimocarpus longan CAT and Nicotiana tabacum CAT1. Expression profile analysis demonstrated that HuCAT3 is mainly expressed in green cotyledons and mature stems, and was regulated by H2O2, drought, cold and salt stress, whereas, its expression patterns and maximum expression levels varied with stress types. HuCAT activity increased as exposure to the tested stresses, and the fluctuation of HuCAT activity was consistent with HuCAT3 mRNA abundance (except for 0.5 days upon drought stress). HuCAT3 mRNA elevations and HuCAT activities changes under cold stress were also in conformity with the cold tolerances among the four genotypes. The obtained results confirmed a major role of HuCAT3 in abiotic stress response of pitaya. This may prove useful in understanding pitaya's high tolerance to abiotic stresses at molecular level.

  5. Energy restriction affect liver development in Hu sheep ram lambs through Hippo signaling pathway.

    Science.gov (United States)

    Zhang, Ting-Ting; Zhang, Guo-Min; Jin, Yu-Hang; Guo, Yi-Xuan; Wang, Zhen; Fan, Yi-Xuan; El-Samahy, M A; Wang, Feng

    2017-09-02

    This study aimed to evaluate the effects of dietary energy restriction on postnatal liver development in Hu sheep ram lambs. A total of 16 ram lambs were randomly divided into two groups: 100% energy requirements diet and 55% energy requirements diet, which were fed for 75 d. Results showed that the final body and liver weights decreased with energy restriction (p Energy restriction caused a significant decrease in the levels of circulating insulin-like growth factor 1 (IGF1) and an increase in growth hormone secretion (p energy restriction, respectively (p energy restriction group (p energy restriction group (p energy restriction in ram lambs impairs liver development by increasing apoptosis, which may occur via the Hippo signaling pathway. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Flight Testing Surfaces Engineered for Mitigating Insect Adhesion on a Falcon HU-25C

    Science.gov (United States)

    Shanahan, Michelle; Wohl, Chris J.; Smith, Joseph G., Jr.; Connell, John W.; Siochi, Emilie J.; Doss, Jereme R.; Penner, Ronald K.

    2015-01-01

    Insect residue contamination on aircraft wings can decrease fuel efficiency in aircraft designed for natural laminar flow. Insect residues can cause a premature transition to turbulent flow, increasing fuel burn and making the aircraft less environmentally friendly. Surfaces, designed to minimize insect residue adhesion, were evaluated through flight testing on a Falcon HU-25C aircraft flown along the coast of Virginia and North Carolina. The surfaces were affixed to the wing leading edge and the aircraft remained at altitudes lower than 1000 feet throughout the flight to assure high insect density. The number of strikes on the engineered surfaces was compared to, and found to be lower than, untreated aluminum control surfaces flown concurrently. Optical profilometry was used to determine insect residue height and areal coverage. Differences in results between flight and laboratory tests suggest the importance of testing in realistic use environments to evaluate the effectiveness of engineered surface designs.

  7. The Curious Case of HU Aquarii - Dynamically Testing Proposed Planetary Systems

    CERN Document Server

    Horner, Jonathan; Marshall, Jonathan P; Tinney, Chris G; Butters, Oliver W

    2012-01-01

    In early 2011, the discovery of two planets moving on surprisingly extreme orbits around the eclipsing polar cataclysmic variable system HU Aquraii was announced based on variations in the timing of mutual eclipses between the two central stars. We perform a detailed dynamical analysis of the stability of the exoplanet system as proposed in that work, revealing that it is simply dynamically unfeasible. We then apply the latest rigorous methods used by the Anglo-Australian Planet Search to analyse radial velocity data to re-examine the data used to make the initial claim. Using that data, we arrive at a significantly different orbital solution for the proposed planets, which we then show through dynamical analysis to be equally unfeasible. Finally, we discuss the need for caution in linking eclipse-timing data for cataclysmic variables to the presence of planets, and suggest a more likely explanation for the observed signal.

  8. Congenital Pelger-Huët anomaly in a Danish/Swedish Farmdog: Case Report

    Directory of Open Access Journals (Sweden)

    Allison Robin W

    2011-03-01

    Full Text Available Abstract A 13 year old Danish/Swedish Farmdog from Denmark was evaluated in a veterinary clinic in Warsaw, Poland for evaluation of an orthopedic problem. Radiographs revealed spondylosis and degenerative vertebral disease, which responded to treatment with anti-inflammatory medications. A predominance of hyposegmented neutrophils and eosinophils containing condensed chromatin and normal cytoplasm were identified on a routine CBC. Follow-up blood film evaluations over the course of 12 months confirmed that the hyposegmented granulocytes persisted. The majority of neutrophils contained Grade 2 nuclei (slightly indented, and the mean nuclear score varied from 1.9 to 2.3. Pelger-Huët anomaly (PHA, presumably congenital, was diagnosed based on persistent hyposegmented granulocytes in the absence of an underlying cause for acquired PHA; genetically related dogs were unavailable for testing to confirm vertical transmission. To the authors' knowledge this is the first report of PHA in a Danish/Swedish Farmdog.

  9. Potential therapeutic use of antibodies directed towards HuIFN-gamma.

    Science.gov (United States)

    Froyen, G; Billiau, A

    1997-01-01

    IFN-gamma is an important regulator of immune responses and inflammation. Studies in animal models of inflammation, autoimmunity, cancer, transplant rejection and delayed-type hypersensitivity have indicated that administration of antibodies against IFN-gamma can prevent the occurrence of diseases or alleviate disease manifestations. Therefore, it is speculated that such antibodies may have therapeutical efficacy in human diseases. Since animal-derived antibodies are immunogenic in patients several strategies are being developed in order to reduce or abolish this human anti-mouse antibody (HAMA) response. In our laboratory, we have constructed a single-chain variable fragment (scFv) derived from a mouse antibody with neutralizing potential for human IFN-gamma. A scFv consists of only variable domains tethered together by a flexible linker. The scFv was demonstrated to neutralize the antiviral activity of HuIFN-gamma in vitro and therefore might be considered as a candidate for human therapy.

  10. La voz y la letra en "Un huésped", de Yoko Tawada

    Directory of Open Access Journals (Sweden)

    María Lucía Correa

    2009-05-01

    Full Text Available Los textos de Yoko Tawada, escritorajaponesa residente en Alemania,hacen presente el desgarramientode quien vive fuera de la lenguamaterna. Es una obra que se balanceasobre el límite entre loscódigos, donde nada hay evidente,donde la palabra parece tener vidapropia. Entre los textos que tomanesta extrañeza del lenguaje comosustrato, se encuentra el relato"Un huésped", cuya particular referenciaa la voz y a la letra comodos registros que se entrelazan yse repelen alternativamente, loconvierte en una valiosa fuente dereflexiones sobre lo oral, la voz, laletra, la lectura y la escritura.Palabras clave: Yoko Tawada, literaturajaponesa contemporánea, voz,letra, escritura.

  11. The SCID-hu mouse as a tool in immunotoxicological risk assessment: Effects of 2-acetyl-4(5)-tetrahydroxybutyl-imidazole (THI) and di-n-butyltin dichloride (DBTC) on the human thymus in SCID-hu mice

    NARCIS (Netherlands)

    Heer, C. de; Schuurman, H.J.; Houben, G.F.; Pieters, R.H.H.; Penninks, A.H.; Loveren, H. van

    1995-01-01

    SCID mice engrafted with human fetal thymus and liver tissue fragments (SCID-hu mice) are currently considered as a new tool in human immunotoxicological risk assessment. Testing of various immunotoxicants exerting thymotoxicity via different intrathymic target cell types is necessary for validation

  12. The complete chloroplast genome sequence of the relict woody plant Metasequoia glyptostroboides Hu et Cheng.

    Science.gov (United States)

    Chen, Jinhui; Hao, Zhaodong; Xu, Haibin; Yang, Liming; Liu, Guangxin; Sheng, Yu; Zheng, Chen; Zheng, Weiwei; Cheng, Tielong; Shi, Jisen

    2015-01-01

    Metasequoia glyptostroboides Hu et Cheng is the only species in the genus Metasequoia Miki ex Hu et Cheng, which belongs to the Cupressaceae family. There were around 10 species in the Metasequoia genus, which were widely spread across the Northern Hemisphere during the Cretaceous of the Mesozoic and in the Cenozoic. M. glyptostroboides is the only remaining representative of this genus. Here, we report the complete chloroplast (cp) genome sequence and the cp genomic features of M. glyptostroboides. The M. glyptostroboides cp genome is 131,887 bp in length, with a total of 117 genes comprised of 82 protein-coding genes, 31 tRNA genes and four rRNA genes. In this genome, 11 forward repeats, nine palindromic repeats, and 15 tandem repeats were detected. A total of 188 perfect microsatellites were detected through simple sequence repeat (SSR) analysis and these were distributed unevenly within the cp genome. Comparison of the cp genome structure and gene order to those of several other land plants indicated that a copy of the inverted repeat (IR) region, which was found to be IR region A (IRA), was lost in the M. glyptostroboides cp genome. The five most divergent and five most conserved genes were determined and further phylogenetic analysis was performed among plant species, especially for related species in conifers. Finally, phylogenetic analysis demonstrated that M. glyptostroboides is a sister species to Cryptomeria japonica (L. F.) D. Don and to Taiwania cryptomerioides Hayata. The complete cp genome sequence information of M. glyptostroboides will be great helpful for further investigations of this endemic relict woody plant and for in-depth understanding of the evolutionary history of the coniferous cp genomes, especially for the position of M. glyptostroboides in plant systematics and evolution.

  13. Comment on "A three-loop radiative neutrino mass model with dark matter" [Phys. Lett. B 741 (2015) 163

    CERN Document Server

    Geng, Chao-Qiang; Tsai, Lu-Hsing

    2015-01-01

    We revisit the calculation of the three-loop diagrams for the radiative neutrino mass generation and consider some relevant constraints on the model recently proposed by L. Jin {\\it et al} [Phys. Lett. B 741 (2015) 163]. We find that the previous approximation is inappropriate due to the neglect of some important contributions, and the benchmark point proposed can neither give rise to enough neutrino masses nor accommodate these additional constraints, such as the validity of the perturbation theory, the electroweak precision measurements, and the neutrinoless double beta decays.

  14. Comment on "Propagation of surface waves on a semi-bounded quantum magnetized collisional plasma" [Phys. Plasmas 20, 122106 (2013)

    Science.gov (United States)

    Moradi, Afshin

    2016-04-01

    In a recent article [Niknam et al., Phys. Plasmas 20, 122106 (2013)], Niknam et al. investigated the propagation of TM surface waves on a semi-bounded quantum magnetized collisional plasma in the Faraday configuration (in this case, the magnetic field is parallel to the both of the plasma surface and direction of propagation). Here, we present a fresh look at the problem and show that TM surface waves cannot propagate on surface of the present system. We find in the Faraday configuration the surface waves acquire both TM and TE components due to the cyclotron motion of electrons. Therefore, the main result of the work by Niknam et al. is incorrect.

  15. Comment on "Surface electromagnetic wave equations in a warm magnetized quantum plasma" [Phys. Plasmas 21, 072114 (2014)

    Science.gov (United States)

    Moradi, Afshin

    2016-07-01

    In a recent article [C. Li et al., Phys. Plasmas 21, 072114 (2014)], Li et al. studied the propagation of surface waves on a magnetized quantum plasma half-space in the Voigt configuration (in this case, the magnetic field is parallel to the surface but is perpendicular to the direction of propagation). Here, we present a fresh look at the problem and obtain a new form of dispersion relation of surface waves of the system. We find that our new dispersion relation does not agree with the result obtained by Li et al.

  16. Comment on “On the quantum theory of molecules” [J. Chem. Phys. 137, 22A544 (2012)

    Energy Technology Data Exchange (ETDEWEB)

    Sutcliffe, Brian T., E-mail: bsutclif@ulb.ac.be [Service de Chimie quantique et Photophysique, Université Libre de Bruxelles, B-1050 Bruxelles (Belgium); Woolley, R. Guy [School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS (United Kingdom)

    2014-01-21

    In our previous paper [B. T. Sutcliffe and R. G. Woolley, J. Chem. Phys. 137, 22A544 (2012)] we argued that the Born-Oppenheimer approximation could not be based on an exact transformation of the molecular Schrödinger equation. In this Comment we suggest that the fundamental reason for the approximate nature of the Born-Oppenheimer model is the lack of a complete set of functions for the electronic space, and the need to describe the continuous spectrum using spectral projection.

  17. Comment on "The physics origin of the hierarchy of bodies in space" [J. Appl. Phys. 119, 094901 (2016)

    Science.gov (United States)

    Swartz, C. H.

    2016-09-01

    A recent paper [A. Bejan and R. W. Wagstaff, J. Appl. Phys. 119, 094901 (2016)] concludes that bodies of the same size suspended uniformly in space are in a state of high internal tension. The tension is then relieved by rearrangement of the bodies into a non-uniform distribution of mass. In this Comment, it is shown that the conclusions are based upon calculations which are in error, and that the amount of tension is not in fact decreased by such a rearrangement.

  18. Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export function.

    Science.gov (United States)

    Yedavalli, Venkat S R K; Neuveut, Christine; Chi, Ya-Hui; Kleiman, Lawrence; Jeang, Kuan-Teh

    2004-10-29

    A single transcript in its unspliced and spliced forms directs the synthesis of all HIV-1 proteins. Although nuclear export of intron-containing cellular transcripts is restricted in mammalian cells, HIV-1 has evolved the viral Rev protein to overcome this restriction for viral transcripts. Previously, CRM1 was identified as a cellular cofactor for Rev-dependent export of intron-containing HIV-1 RNA. Here, we present evidence that Rev/CRM1 activity utilizes the ATP-dependent DEAD box RNA helicase, DDX3. We show that DDX3 is a nucleo-cytoplasmic shuttling protein, which binds CRM1 and localizes to nuclear membrane pores. Knockdown of DDX3 using either antisense vector or dominant-negative mutants suppressed Rev-RRE-function in the export of incompletely spliced HIV-1 RNAs. Plausibly, DDX3 is the human RNA helicase which functions in the CRM1 RNA export pathway analogously to the postulated role for Dbp5p in yeast mRNA export.

  19. Horns Rev 2 offshore wind farm photo case with wakes observed in 2016

    DEFF Research Database (Denmark)

    Hasager, Charlotte Bay; Nygaard, Nicolai Gayle; Volker, Patrick

    Offshore wind farm wakes were photographed in foggy conditions at Horns Rev 2 on 25 January 2016 at 12:45 UTC (See1). The study examines the atmospheric conditions from satellite images, radiosonde, vertical profiling wind lidar located at the transformer platform and SCADA data. Furthermore...

  20. Multiple roles of Rev3, the catalytic subunit of polzeta in maintaining genome stability in vertebrates

    NARCIS (Netherlands)

    E. Sonoda (Eiichiro); S. Takeda (Shiunichi); T. Okada (Takashi); G.Y. Zhao (Guang); S. Tateishi (Satoshi); K. Araki (Kasumi); M. Yamaizumi (Masaru); T. Yagi (Takashi); N.S. Verkaik (Nicole); D.C. van Gent (Dik); M. Takata (Minoru)

    2003-01-01

    textabstractTranslesion DNA synthesis (TLS) and homologous DNA recombination (HR) are two major postreplicational repair (PRR) pathways. The REV3 gene of Saccharomyces cerevisiae encodes the catalytic subunit of DNA polymerase zeta, which is involved in mutagenic TLS. To investigat

  1. Power deficits due to wind turbine wakes at Horns Rev wind farm

    DEFF Research Database (Denmark)

    Hansen, Kurt Schaldemose; Barthelmie, Rebecca; Jensen, Leo E.

    2010-01-01

    The wind turbine operational charac-teristics, power measurements and the meteorological measurements as 10 minute statistical data from Horns Rev offshore wind farm have been identified, synchronized, quality screened and stored in a common database. A number of flow cases have been identified...

  2. TextRev: A Window into How General and Organic Chemistry Students Use Textbook Resources.

    Science.gov (United States)

    Smith, Bradley D.; Jacobs, Dennis C.

    2003-01-01

    Points out the important use of textbooks and their ancillary resources in lower-division chemistry courses and the scientific misconceptions found in them. Introduces the TextRev Project which is a new resource for data collection and analysis. Investigates how first and second year chemistry students use and value their textbooks and their study…

  3. Regulation of bile acid synthesis by the nuclear receptor Rev-erb alpha

    NARCIS (Netherlands)

    Duez, Helene; Van Der Veen, Jelske N.; Duhem, Christian; Pourcet, Benoit; Touvier, Thierry; Fontaine, Coralie; Derudas, Bruno; Bauge, Eric; Havinga, Rick; Bloks, Vincent W.; Wolters, Henk; Van Der Sluijs, Fjodor H.; Vennstrom, Bjorn; Kuipers, Folkert; Staels, Bart

    2008-01-01

    Background & Aims: Conversion into bile acids represents an important route to remove excess cholesterol from the body. Rev-erb alpha is a nuclear receptor that participates as one of the clock genes in the control of circadian rhythmicity and plays a regulatory role in lipid metabolism and adipogen

  4. Determining the REV for Fracture Rock Mass Based on Seepage Theory

    Directory of Open Access Journals (Sweden)

    Lili Zhang

    2017-01-01

    Full Text Available Seepage problems of the fractured rock mass have always been a heated topic within hydrogeology and engineering geology. The equivalent porous medium model method is the main method in the study of the seepage of the fractured rock mass and its engineering application. The key to the method is to determine a representative elementary volume (REV. The FractureToKarst software, that is, discrete element software, is a main analysis tool in this paper and developed by a number of authors. According to the standard of rock classification established by ISRM, this paper aims to discuss the existence and the size of REV of fractured rock masses with medium tractility and provide a general method to determine the existence of REV. It can be gleaned from the study that the existence condition of fractured rock mass with medium tractility features average fracture spacing smaller than 0.6 m. If average fracture spacing is larger than 0.6 m, there is no existence of REV. The rationality of the model is verified by a case study. The present research provides a method for the simulation of seepage field in fissured rocks.

  5. Antidepressant effects of curcumin and HU-211 coencapsulated solid lipid nanoparticles against corticosterone-induced cellular and animal models of major depression

    Science.gov (United States)

    He, Xiaolie; Zhu, Yanjing; Wang, Mei; Jing, Guoxin; Zhu, Rongrong; Wang, Shilong

    2016-01-01

    Major depression is a complex neuropsychiatric disorder with few treatment approaches. The use of nontargeted antidepressants induced many side effects with their low efficacy. A more precise targeting strategy is to develop nanotechnology-based drug delivery systems; hence, we employed solid lipid nanoparticles (SLNs) to encapsulate HU-211 and curcumin (Cur). The antidepressant effects of the dual-drug nanoparticles (Cur/SLNs-HU-211) for major depression treatment were investigated in corticosterone-induced cellular and animal models of major depression. Cur/SLNs-HU-211 can effectively protect PC12 cells from corticosterone-induced apoptosis and can release more dopamine, which may be associated with the higher uptake of Cur/SLNs-HU-211 shown by cellular uptake behavior analysis. Additionally, Cur/SLNs-HU-211 significantly reduced the immobility time in forced swim test, enhanced fall latency in rotarod test, and improved the level of dopamine in mice blood. Cur/SLNs-HU-211 can deliver more Cur to the brain and thus produce a significant increase in neurotransmitters level in brain tissue, especially in the hippocampus and striatum. The results of Western blot and immunofluorescence revealed that Cur/SLNs-HU-211 can significantly enhance the expression of CB1, p-MEK1, and p-ERK1/2. Our study suggests that Cur/SLNs-HU-211 may have great potential for major depression treatment. PMID:27757031

  6. Offshore wind farms in the local community - a survey at Horns Rev wind farm. Background report; Havvindmoeller i lokalomraedet - en undersoegelse ved Horns Rev havmoellepark. Baggrundsrapport

    Energy Technology Data Exchange (ETDEWEB)

    Kuehn, S.

    2005-07-15

    This report is part of a socio-economic project about offshore wind farms' influence on the local community. The project is part of the monitoring programme in connection with the establishment of the offshore wind farms at Horns Rev and Nysted. The socio-economic project consists of a sociological and an environment-economic project. This report is part of the sociological project which has the purpose of identifying attitudes towards the wind farm before and after the erection with a view to identify possible changes in opinions and reasons for these changes. (BA)

  7. HuD interacts with Bdnf mRNA and is essential for activity-induced BDNF synthesis in dendrites.

    Directory of Open Access Journals (Sweden)

    Filip Vanevski

    Full Text Available Highly specific activity-dependent neuronal responses are necessary for modulating synapses to facilitate learning and memory. We present evidence linking a number of important processes involved in regulating synaptic plasticity, suggesting a mechanistic pathway whereby activity-dependent signaling, likely through protein kinase C (PKC-mediated phosphorylation of HuD, can relieve basal repression of Bdnf mRNA translation in dendrites, allowing for increased TrkB signaling and synaptic remodeling. We demonstrate that the neuronal ELAV family of RNA binding proteins associates in vivo with several Bdnf mRNA isoforms present in the adult brain in an activity-dependent manner, and that one member, HuD, interacts directly with sequences in the long Bdnf 3' untranslated region (3'UTR and co-localizes with Bdnf mRNA in dendrites of hippocampal neurons. Activation of PKC leads to increased dendritic translation of mRNAs containing the long Bdnf 3'UTR, a process that is dependent on the presence of HuD and its phosphorylation at threonine residues 149 and/or 165. Thus, we found a direct effect of HuD on regulating translation of dendritic Bdnf mRNAs to mediate local and activity-dependent increases in dendritic BDNF synthesis.

  8. Genetic diversity and population structure in the narrow endemic Chinese walnut Juglans hopeiensis Hu: implications for conservation

    Science.gov (United States)

    Yiheng Hu; Meng Dang; Xiaojia Feng; Keith Woeste; Peng Zhao

    2017-01-01

    The conservation of narrow endemic species relies on accurate information regarding their population structure. Juglans hopeiensis Hu (Ma walnut), found only in Hebei province, Beijing, and Tianjin, China, is a threatened tree species valued commercially for its nut and wood. Sequences of two maternally inherited mitochondrial markers and two...

  9. Hard bottom substrate monitoring Horns Rev offshore wind farm. Annual status report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S.B.; Pedersen, John

    2004-05-15

    Elsam and Eltra built the offshore demonstration wind farm at Horns Rev in the North Sea. Elsam is the owner and is responsible for the operation of the wind farm. Eltra is responsible for the connection of the wind farm to the national onshore grid. In the summer months of 2002, Elsam constructed the world's largest offshore wind farm off the Danish west coast. The wind farm is sited 14-20 km into the North Sea, west of Blaevands Huk. The first wind turbine was erected in May 2002 and the last wind turbine tower of a total of 80 was in place by August 2002. The construction work was completed with the last connecting cables sluiced down in September 2002. All the wind turbines were in production by December 2002. The expected impact of the wind farm will primarily be an alternation of habitats due to the introduction of hard bottom substrates as wind turbine towers and scour protections. A continuous development in the epifouling communities will be expected together with an introduction of new or alien species in the area. The indigenous benthic community in the area of Horns Rev can be characterised by infauna species belonging to the Goniadella-Spisula community. This community is typical of sandbanks in the North Sea area, although communities in such areas are very variable and site-specific. Character species used as indicators for environmental changes in the Horns Rev area are the bristle worms Goniadella bobretzkii, Ophelia borealis, Psione remota and Orbinia sertulata and the mussels Goodallia triangularis and Spisula solida. In connection with the implementation of the monitoring programme concerning the ecological impact of the introduction of hard substrate related to the Horns Rev Wind Farm, surveys on hard bottom substrate was conducted in March 2003 and in September 2003. This report describes the first year results of surveys on hard substrate after the completion of the offshore wind farm at Horns Rev. (au)

  10. Peptides derived from HIV-1 integrase that bind Rev stimulate viral genome integration.

    Directory of Open Access Journals (Sweden)

    Aviad Levin

    Full Text Available BACKGROUND: The human immunodeficiency virus type 1 (HIV-1 integrase protein (IN, catalyzes the integration of viral DNA into the host cell genome. IN catalyzes the first step of the integration process, namely the 3'-end processing in which IN removes a pGT dinucleotide from the 3' end of each viral long terminal repeat (LTR. Following nuclear import of the viral preintegration complex, the host chromosomal DNA becomes accessible to the viral cDNA and the second step of the integration process, namely the strand-transfer step takes place. This ordered sequence of events, centered on integration, is mandatory for HIV replication. METHODOLOGY/PRINCIPAL FINDINGS: Using an integrase peptide library, we selected two peptides, designated INr-1 and INr-2, which interact with the Rev protein and probably mediate the Rev-integrase interaction. Using an in-vitro assay system, we show that INr-1 and INr-2 are able to abrogate the inhibitory effects exerted by Rev and Rev-derived peptides on integrase activity. Both INr-1 and INr-2 were found to be cell-permeable and nontoxic, allowing a study of their effect in HIV-1-infected cultured cells. Interestingly, both INr peptides stimulated virus infectivity as estimated by production of the viral P24 protein, as well as by determination of the appearance of newly formed virus particles. Furthermore, kinetics studies revealed that the cell-permeable INr peptides enhance the integration process, as was indeed confirmed by direct determination of viral DNA integration by real-time PCR. CONCLUSIONS/SIGNIFICANCE: The results of the present study raise the possibility that in HIV-infected cells, the Rev protein may be involved in the integration of proviral DNA by controlling/regulating the activity of the integrase. Release from such inhibition leads to stimulation of IN activity and multiple viral DNA integration events.

  11. Lasers (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Hellman, Hal

    1969-01-01

    A laser is an instrument that produces an enormously intense pencil-thin beam of light. In this booklet we shall learn what there is about the laser that gives it so much promise. We shall investigate what it is, how it works, and the different kinds of lasers there are.

  12. Computers (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Corliss, William R

    1967-01-01

    This booklet relates how wires, transistors, and human ingenuity are combined to produce machines that surpass all the calculating prodigies that ever lived in speed, accuracy, and stamina, though perhaps not in the matter of mystery.

  13. Computers (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Corliss, William R

    1967-01-01

    This booklet relates how wires, transistors, and human ingenuity are combined to produce machines that surpass all the calculating prodigies that ever lived in speed, accuracy, and stamina, though perhaps not in the matter of mystery.

  14. A cell-penetrating antibody fragment against HIV-1 Rev has high antiviral activity: characterization of the paratope.

    Science.gov (United States)

    Zhuang, Xiaolei; Stahl, Stephen J; Watts, Norman R; DiMattia, Michael A; Steven, Alasdair C; Wingfield, Paul T

    2014-07-18

    The HIV-1 protein Rev oligomerizes on viral transcripts and directs their nuclear export. Previously, a Fab against Rev generated by phage display was used to crystallize and solve the structure of the Rev oligomerization domain. Here we have investigated the capability of this Fab to block Rev oligomerization and inhibit HIV-1 replication. The Fab itself did not have antiviral activity, but when a Tat-derived cell-penetrating peptide was appended, the resulting molecule (FabRev1-Tat) was strongly inhibitory of three different CCR5-tropic HIV-1 isolates (IC50 = 0.09-0.44 μg/ml), as assessed by suppression of reverse transcriptase activity in infected peripheral blood mononuclear cells, and had low cell toxicity (TC50 > 100 μg/ml). FabRev1-Tat was taken up by both peripheral blood mononuclear and HEK293T cells, appearing in both the cytoplasm and nucleus, as shown by immunofluorescence confocal laser scanning microscopy. Computational alanine scanning was used to identify key residues in the complementarity-determining regions to guide mutagenesis experiments. Residues in the light chain CDR3 (LCDR3) were assessed to be important. Residues in LCDR3 were mutated, and LCDR3-Tyr(92) was found to be critical for binding to Rev, as judged by surface plasmon resonance and electron microscopy. Peptides corresponding to all six CDR regions were synthesized and tested for Rev binding. None of the linear peptides had significant affinity for Rev, but four of the amide-cyclic forms did. Especially cyclic-LCDR3 (LGGYPAASYRTA) had high affinity for Rev and was able to effectively depolymerize Rev filaments, as shown by both surface plasmon resonance and electron microscopy.

  15. The Preliminary Research on Rupture and Factors Affecting Rupturing of the Hoh Sai Hu Segment in Eastern Kunlun

    Institute of Scientific and Technical Information of China (English)

    Bai Yuzhu; Xu Jie; Zhou Qing; Zhou Bengang

    2012-01-01

    On November 14, 2001, an extraordinarily large earthquake (Ms8. 1) occurred on the Hoh Sai Hu segment of the Eastern Kunlun Fault, in the northern Qinghai-Tibetan Plateau. The seismogenic fault, the Hoh Sai Hu segment, is a left-lateral fault with a high slip rate in geological history, with the average slip rate reaching( 14. 8± 2. 8)mm/a since the late Pleistocene. Different slip rates of the Hoh Sai Hu segment can affect fault motion in the future. Therefore, this paper analyzes the effect of different slip rates and different initial friction coefficients on the fault plane of the Hoh Sai Hu segment of the eastern Kunlun Fault on the rupture behaviors of the fault. In this research, we apply the single degree of spring block model controlled by the rate- and state-dependent frictional constitutive laws. Using the fault dislocation model and based on ancient earthquake research, historical earthquakes data and the achievements of previous researchers, we obtained the parameters of this model. Through numerical simulation of the rupturing motion of the Hoh Sai Hu segment in the next 6500 years under different slip rates, we find that a faster annual slip rate will shorten the recurrence interval of the earthquake. For example, the earthquake recurrence interval is 2100a at a slip rate of 0. 014m/a, which agrees with previous research, but, the recurrence interval will be 1000 - 1500a and 2100 - 2500a, corresponding to the slip rates of 0. 018m/a and 0. 008m/a, respectively. The fault slip rate has no regular effect on the coseismic slip rate and fault displacement in an earthquake. The initial friction coefficient on the fault surface has an effect on the earthquake recurrence interval. A smaller initial friction coefficient will lengthen the earthquake recurrence interval. At the same time, the smaller initial friction coefficient will lead to larger slip rates and fault displacement when earthquakes occur.

  16. Striatal synaptic dysfunction and hippocampal plasticity deficits in the Hu97/18 mouse model of Huntington disease.

    Directory of Open Access Journals (Sweden)

    Karolina Kolodziejczyk

    Full Text Available Huntington disease (HD is a fatal neurodegenerative disorder caused by a CAG repeat expansion in the gene (HTT encoding the huntingtin protein (HTT. This mutation leads to multiple cellular and synaptic alterations that are mimicked in many current HD animal models. However, the most commonly used, well-characterized HD models do not accurately reproduce the genetics of human disease. Recently, a new 'humanized' mouse model, termed Hu97/18, has been developed that genetically recapitulates human HD, including two human HTT alleles, no mouse Hdh alleles and heterozygosity of the HD mutation. Previously, behavioral and neuropathological testing in Hu97/18 mice revealed many features of HD, yet no electrophysiological measures were employed to investigate possible synaptic alterations. Here, we describe electrophysiological changes in the striatum and hippocampus of the Hu97/18 mice. At 9 months of age, a stage when cognitive deficits are fully developed and motor dysfunction is also evident, Hu97/18 striatal spiny projection neurons (SPNs exhibited small changes in membrane properties and lower amplitude and frequency of spontaneous excitatory postsynaptic currents (sEPSCs; however, release probability from presynaptic terminals was unaltered. Strikingly, these mice also exhibited a profound deficiency in long-term potentiation (LTP at CA3-to-CA1 synapses. In contrast, at 6 months of age we found only subtle alterations in SPN synaptic transmission, while 3-month old animals did not display any electrophysiologically detectable changes in the striatum and CA1 LTP was intact. Together, these data reveal robust, progressive deficits in synaptic function and plasticity in Hu97/18 mice, consistent with previously reported behavioral abnormalities, and suggest an optimal age (9 months for future electrophysiological assessment in preclinical studies of HD.

  17. Alternativa terapeutica en la isquemia cerebral empleando la Eritropoyetina humana recombinante (rHu-EPO CIM como neuroprotector.

    Directory of Open Access Journals (Sweden)

    Iliana Sosa Testé

    2005-01-01

    Full Text Available Las enfermedades vasculares constituyen las terceras causas de muerte en Cuba. Su variante isquémica es causa de invalidez y perdida de la calidad de la vida. La búsqueda de agentes neuroprotectores es muy importante sí tenemos en cuenta que nuestra población está envejecida. Muchos son las razones por la que una molécula con propiedades neuroprotectoras en los modelos animales no tenga los resultados en ensayos clínicos en humanos. Este trabajo analiza y discute como mejorar estos aspectos. La Eritropoyetina humana recombinante (rHu EPO, conocido por su papel en la diferenciación del eritrocito, ha mostrado ser neuroprotector durante la isquemia cerebral en los modelos de animales adultos. De esta molécula se exponen los mecanismos de acción, que son las bases de los resultados positivos alcanzaron en las investigaciones preclínicas y los ensayos clínicos, que lo proponen como el neuroprotector ideal. Finalmente se comparan los resultados alcanzados por los autores en las investigaciones con la rHu EPO producida por el Centro Molecular de Inmunología de Cuba (CIM. El efecto de la aplicación de rHu EPO se evidenció por la disminución del edema cerebral, preservación de la conducta exploratoria y la disminución de la incidencia de señales clínicas de infarto cerebral y alteraciones patológicas. El grupo de resultados demostró las diferencias significantes entre los animales controles y tratados con el rHu EPO con los no tratados que sugieren que el rHu EPO debe ser considerado como una alternativa terapéutica contra el infarto cerebral en su fase aguda.

  18. A novel high throughput biochemical assay to evaluate the HuR protein-RNA complex formation.

    Directory of Open Access Journals (Sweden)

    Vito G D'Agostino

    Full Text Available The RNA binding protein HuR/ELAVL1 binds to AU-rich elements (AREs promoting the stabilization and translation of a number of mRNAs into the cytoplasm, dictating their fate. We applied the AlphaScreen technology using purified human HuR protein, expressed in a mammalian cell-based system, to characterize in vitro its binding performance towards a ssRNA probe whose sequence corresponds to the are present in TNFα 3' untranslated region. We optimized the method to titrate ligands and analyzed the kinetic in saturation binding and time course experiments, including competition assays. The method revealed to be a successful tool for determination of HuR binding kinetic parameters in the nanomolar range, with calculated Kd of 2.5±0.60 nM, k on of 2.76±0.56*10(6 M(-1 min(-1, and k off of 0.007±0.005 min(-1. We also tested the HuR-RNA complex formation by fluorescent probe-based RNA-EMSA. Moreover, in a 384-well plate format we obtained a Z-factor of 0.84 and an averaged coefficient of variation between controls of 8%, indicating that this biochemical assay fulfills criteria of robustness for a targeted screening approach. After a screening with 2000 small molecules and secondary verification with RNA-EMSA we identified mitoxantrone as an interfering compound with rHuR and TNFα probe complex formation. Notably, this tool has a large versatility and could be applied to other RNA Binding Proteins recognizing different RNA, DNA, or protein species. In addition, it opens new perspectives in the identification of small-molecule modulators of RNA binding proteins activity.

  19. 75 FR 32493 - NUREG-0654/FEMA-REP-, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-06-08

    ... SECURITY Federal Emergency Management Agency NUREG-0654/FEMA-REP-, Rev. 1, Supplement 3, Guidance for... be held to discuss the proposed Supplement 3 to NUREG-0654/FEMA-REP-1, Rev.1, Guidance for Protective Action Recommendations for General Emergencies (NUREG Supp 3). NUREG Supp 3 addresses onsite and...

  20. 48 CFR 53.301-1415 - SF 1415 (Rev. 7/93) Consent of Surety and Increase of Penalty.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false SF 1415 (Rev. 7/93) Consent of Surety and Increase of Penalty. 53.301-1415 Section 53.301-1415 Federal Acquisition Regulations...-1415 SF 1415 (Rev. 7/93) Consent of Surety and Increase of Penalty. EC01MY91.102...

  1. 78 FR 63964 - Request for Comments on Draft NIST Interagency Report (NISTIR) 7628 Rev. 1, Guidelines for Smart...

    Science.gov (United States)

    2013-10-25

    ... (NISTIR) 7628 Rev. 1, Guidelines for Smart Grid Cyber Security AGENCY: National Institute of Standards and... Smart Grid Cyber Security. Draft NISTIR 7628 Rev. 1 was completed by the NIST-led Smart Grid Cybersecurity Committee (formerly the Cyber Security Working Group) of the Smart Grid Interoperability...

  2. A new phantom for image quality, geometric destortion, and HU calibration in MSCT and CBCT

    Science.gov (United States)

    Voigt, Johannes M.; Blendl, Christian; Selbach, Markus; Uphoff, Clemens; Fiebich, Martin

    2012-03-01

    Flat panel cone-beam computed tomography (CBCT) is developing to the state-of-the-art technique in several medical disciplines such as dental and otorhinolaryngological imaging. Dental and otorhinolaryngological CBCT systems offer a variety of different field-of-view sizes from 6.0 to 17.0 cm. Standard phantoms are only designed for the use in multi-slices CT (MSCT) and there is no phantom which provides detail structures for all common characteristic values and Hounsfield calibration. In this study we present a new phantom specially designed for use with MSCT and CBCT systems providing detail structures for MTF, 3D MTF, NPS, SNR, geometric distortion and HU calibration. With this phantom you'll only need one acquisition for image quality investigation and assurance. Materials and methods: The phantom design is shown in figure 1. To investigate the practicability, the phantom was scanned using dedicated MSCT-scanners, 3D C-arms und digital volume tomographs. The acquired axial image stacks were analyzed using a dedicated computer program, which is provided as an ImageJ plugin. The MTF was compared to other methodologies such as a thin wire, a sphere or noise response [10, 13, 14]. The HU values were also computed using other common methods. Results: These results are similar to the results of others studies [10, 13, 14]. The method has proven to be stable and delivers comparable results to other methodologies such as using a thin wire. The NPS was calculated for all materials. Furthermore, CT numbers for all materials were computed and compared to the desired values. The measurement of geometric deformation has proven to be accurate. Conclusion: A unique feature of this phantom is to compute the geometric deformation of the 3D-volume image. This offers the chance to improve accuracy, e.g. in dental implant planning. Another convenient feature is that the phantom needs to be scanned only once with otorhinolaryngological volume tomographs to be fully displayed. It is

  3. {sup 89}Zr-huJ591 immuno-PET imaging in patients with advanced metastatic prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Pandit-Taskar, Neeta; Solomon, Stephen B.; Durack, Jeremy C.; Carrasquillo, Jorge A.; Lefkowitz, Robert A.; Osborne, Joseph R. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); O' Donoghue, Joseph A. [Memorial Sloan Kettering Cancer Center, Department of Medical Physics, New York, NY (United States); Beylergil, Volkan; Ruan, Shutian; Cheal, Sarah M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Lyashchenko, Serge [Memorial Sloan Kettering Cancer Center, Department of Radiochemistry and Molecular Imaging Probes Core, New York, NY (United States); Gonen, Mithat [Memorial Sloan Kettering Cancer Center, Department of Epidemiology and Biostatistics, New York, NY (United States); Lewis, Jason S. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Department of Radiochemistry and Molecular Imaging Probes Core, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Program in Molecular Pharmacology and Chemistry, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); Holland, Jason P. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Harvard Medical School, Department of Radiology of Massachusetts General Hospital, Boston, MA (United States); Reuter, Victor E. [Memorial Sloan Kettering Cancer Center, Department of Pathology, New York, NY (United States); Weill Cornell Medical College, Department of Pathology, New York, NY (United States); Loda, Massimo F. [Dana-Farber Cancer Institute, Boston, MA (United States); Broad Institute of Harvard and MIT, Cambridge, MA (United States); Smith-Jones, Peter M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Department of Psychiatry and Behavioral Science of Stony Brook University, Stony Brook, NY (United States); Weber, Wolfgang A.; Larson, Steven M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Program in Molecular Pharmacology and Chemistry, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); Bander, Neil H. [Memorial Sloan Kettering Cancer Center, Department of Surgery, New York, NY (United States); Weill Cornell Medical College, Department of Urology, New York, NY (United States); Scher, Howard I.; Morris, Michael J. [Memorial Sloan Kettering Cancer Center, Department of Medicine, New York, NY (United States); Weill Cornell Medical College, Department of Medicine, New York, NY (United States)

    2014-11-15

    Given the bone tropism of prostate cancer, conventional imaging modalities poorly identify or quantify metastatic disease. {sup 89}Zr-huJ591 positron emission tomography (PET) imaging was performed in patients with metastatic prostate cancer to analyze and validate this as an imaging biomarker for metastatic disease. The purpose of this initial study was to assess safety, biodistribution, normal organ dosimetry, and optimal imaging time post-injection for lesion detection. Ten patients with metastatic prostate cancer received 5 mCi of {sup 89}Zr-huJ591. Four whole-body scans with multiple whole-body count rate measurements and serum activity concentration measurements were obtained in all patients. Biodistribution, clearance, and lesion uptake by {sup 89}Zr-huJ591 immuno-PET imaging was analyzed and dosimetry was estimated using MIRD techniques. Initial assessment of lesion targeting of {sup 89}Zr-huJ591 was done. Optimal time for imaging post-injection was determined. The dose was well tolerated with mild chills and rigors seen in two patients. The clearance of {sup 89}Zr-huJ591 from serum was bi-exponential with biological half-lives of 7 ± 4.5 h (range 1.1-14 h) and 62 ± 13 h (range 51-89 h) for initial rapid and later slow phase. Whole-body biological clearance was 219 ± 48 h (range 153-317 h). The mean whole-body and liver residence time was 78.7 and 25.6 h, respectively. Dosimetric estimates to critical organs included liver 7.7 ± 1.5 cGy/mCi, renal cortex 3.5 ± 0.4 cGy/mCi, and bone marrow 1.2 ± 0.2 cGy/mCi. Optimal time for patient imaging after injection was 7 ± 1 days. Lesion targeting of bone or soft tissue was seen in all patients. Biopsies were performed in 8 patients for a total 12 lesions, all of which were histologically confirmed as metastatic prostate cancer. One biopsy-proven lesion was not positive on {sup 89}Zr-huJ591, while the remaining 11 lesions were {sup 89}Zr-huJ591 positive. Two biopsy-positive nodal lesions were noted only on

  4. Identification of Brucella melitensis Rev.1 vaccine-strain genetic markers: Towards understanding the molecular mechanism behind virulence attenuation.

    Science.gov (United States)

    Issa, Mohammad Nouh; Ashhab, Yaqoub

    2016-09-22

    Brucella melitensis Rev.1 is an avirulent strain that is widely used as a live vaccine to control brucellosis in small ruminants. Although an assembled draft version of Rev.1 genome has been available since 2009, this genome has not been investigated to characterize this important vaccine. In the present work, we used the draft genome of Rev.1 to perform a thorough genomic comparison and sequence analysis to identify and characterize the panel of its unique genetic markers. The draft genome of Rev.1 was compared with genome sequences of 36 different Brucella melitensis strains from the Brucella project of the Broad Institute of MIT and Harvard. The comparative analyses revealed 32 genetic alterations (30 SNPs, 1 single-bp insertion and 1 single-bp deletion) that are exclusively present in the Rev.1 genome. In silico analyses showed that 9 out of the 17 non-synonymous mutations are deleterious. Three ABC transporters are among the disrupted genes that can be linked to virulence attenuation. Out of the 32 mutations, 11 Rev.1 specific markers were selected to test their potential to discriminate Rev.1 using a bi-directional allele-specific PCR assay. Six markers were able to distinguish between Rev.1 and a set of control strains. We succeeded in identifying a panel of 32 genome-specific markers of the B. melitensis Rev.1 vaccine strain. Extensive in silico analysis showed that a considerable number of these mutations could severely affect the function of the associated genes. In addition, some of the discovered markers were able to discriminate Rev.1 strain from a group of control strains using practical PCR tests that can be applied in resource-limited settings.

  5. Mechanism of RNA binding protein HuR in regulating the activity of prostate cancer cells%RNA结合蛋白HuR调控前列腺癌细胞活力机制的研究

    Institute of Scientific and Technical Information of China (English)

    周宁; 程娟; 钟毅; 冉新建; 于文燕; 马琪

    2016-01-01

    目的 前列腺癌在我国的发病率及病死率逐年上升,但其发病机制尚未明确,本研究从转录后水平初步探讨RNA结合蛋白HuR参与调控前列腺癌细胞活力的机制.方法 免疫荧光检测前列腺增生细胞(BPH)及前列腺癌细胞(PC3)中HuR蛋白的定位,蛋白质印迹法检测HuR及环氧合酶-2(cyclooxygenase,COX-2)蛋白在细胞内的表达,MTT法检测细胞活力,脂质体转染法转染质粒及干扰片段,qPCR检测干扰效率,RNA-pull down实验验证HuR对COX-2的调控.结果 HuR在BPH细胞主要定位于细胞核,在PC3细胞中主要定位于胞质.PC3细胞中HuR表达水平为1.699±0.011,高于BPH细胞的0.654±0.028,差异有统计学意义,t=58.67,P<0.001.PC3细胞中过表达HuR后,转染空质粒组细胞活力为1.038±0.117,转染HuR表达质粒后细胞活力为1.838±0.057,差异有统计学意义,t=10.656,P<0.001.干扰HuR表达后,转染siNC组细胞活力为1.254±0.095,转染siHuR干扰片段组细胞活力为0.66±0.102,t=7.383,P=0.002;BPH组和PC3组COX-2表达水平分别为0.449±0.055和1.066±0.068,t=12.147,P<0.001;过表达COX-2后PC3细胞活力明显增加,转染空质粒组细胞活力为1.296±0.114,转染COX-2表达质粒后细胞活力为1.954±0.062,t=18.062,P<0.001.干扰COX-2表达后,PC3细胞活力明显降低,转染siNC组细胞活力为1.233±0.145,转染siCOX-2干扰片段后细胞活力为0.661±0.096,t=5.688,P=0.005.HuR可以结合于COX-2的3′-UTR并上调COX-2蛋白表达,转染空质粒组COX-2蛋白表达水平为0.638±0.067,转染HuR表达质粒组为1.703±0.022,t=26.26,P<0.001.结论 HuR参与调控前列腺癌细胞活力可能是通过上调COX-2表达而实现.

  6. Administrative Cicular No. 31 (Rev. 2) - International indemnity and non-resident allowance

    CERN Multimedia

    Department Head Office - HR Department

    2016-01-01

    Administrative Circular No. 31 (Rev. 2) entitled "International indemnity and non-resident allowance", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 23 June 2016, will be available on 1st September 2016 via the following link: https://cds.cern.ch/record/2208547.   This revised circular cancels and replaces Administrative Circular No. 31 (Rev. 1) also entitled "International indemnity and non-resident allowance" of October 2007. The main changes reflect the decision taken in the framework of the five-yearly review to extend eligibility for international indemnity to all staff members, as well to introduce a distinction between current staff members and those recruited as from 1st September 2016. For the latter, the international indemnity will be calculated as a percentage of the minimum salary of the grade into which they are recruited; the amount granted to the former will not change, and is now expressed ...

  7. The "effects" of Rev-1 vaccination of sheep and goats on human brucellosis in Greece.

    Science.gov (United States)

    Minas, A; Minas, M; Stournara, A; Tselepidis, S

    2004-06-10

    Vaccination of young animals (3-6-month-old sheep and goats) with Rev-1 vaccine for 15 years in Greece, importantly decreased the abortions in sheep and goats as well as the incidence of brucellosis in humans. After the stop of vaccination in 1994, all over Greece, the prevalence of brucellosis in animals and the incidence in humans quickly increased. It was a positive rank correlation (0.90) among these variables. Once an emergency mass-vaccination programme of young and adult animals with Rev-1 vaccine was started in 1998, the human incidence again decreased. The association of the vaccination coverage of animals and incidence of brucellosis in humans was not linear; the decrease in human brucellosis incidence was observed when the vaccination coverage of animals was >30%.

  8. Administrative Circular No. 11 (Rev. 3) - Categories of members of the personnel

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 11 (Rev. 3) entitled “Categories of members of the personnel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 3 July 2014 and entering into force on 1 September 2014, is available on the intranet site of the Human Resources Department.   This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 11 (Rev. 2) entitled “Categories of members of the personnel” of January 2013. The circular was revised in order to include a minor adjustment of the determination of required period of break in the payment of subsistence allowance to certain categories of associated members of the personnel (taking account of possible technical means of control). Furthermore, the possibility of traineeships of long duration was restricted to cases in which the traineeship is awarded pursuant to an agreement between CERN and a...

  9. Administrative Circular No. 13 (Rev. 3) - Guarantees for representatives of the personnel

    CERN Multimedia

    2014-01-01

    Administrative Circular No. 13 (Rev. 3) entitled “Guarantees for representatives of the personnel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 5 December 2013 and entering into force on 1 January 2014, is available on the intranet site of the Human Resources Department (see here).   This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 13 (Rev. 2) entitled “Guarantees for members of the personnel holding representative office” of November 1992. The circular was revised in order to adapt the time given to the representatives of the personnel to perform their elective mandate and to ensure more transparency in their activities, by indicating, inter alia, the percentage of time worked in the framework of their mandate, as well as the training, activities and ensuing results. Department Head Office HR Department

  10. A Circular Motion Activity with Hot Wheels® Rev-Ups

    Science.gov (United States)

    Wagner, Glenn

    2009-02-01

    Hot Wheels® Rev-Ups provide a pedagogically engaging and inexpensive culminating activity for the application of circular motion with constant speed in introductory mechanics. The introductory Rev-Up, shown in Fig. 1, consists of a very durable car with two strong magnets built into the front and back of the car. The track is a piece of flexible plastic with a built-in metallic strip through its center that can then be formed into a circle. Pushing the car forward several times on a flat surface allows the car to move in a vertical circle when placed inside the track. What makes this toy attractive is that the gearing system allows the car to move at a relatively constant speed for about three to five seconds before slowing down appreciably.

  11. Portable Tester Friction (PFT). Mesures du coefficient de frottement dynamique de revêtements de sol

    OpenAIRE

    Marchal, P.; Jacques, M.

    2014-01-01

    Le Portable Tester Friction (PFT) est un appareil destiné à mesurer le coefficient de frottement dynamique d’un revêtement de sol en laboratoire et sur site. Ce document décrit le protocole de mesure avec le PFT et les résultats de mesures effectuées sur un panel de quinze revêtements de sol de caractéristiques différentes (matériaux, états de surface) réalisées en présence de deux polluants (huile et solution d’eau). Le PFT présente une très bonne sensibilité et une très bonne répétabilité. ...

  12. RevTrans: multiple alignment of coding DNA from aligned amino acid sequences

    DEFF Research Database (Denmark)

    Wernersson, Rasmus; Pedersen, Anders Gorm

    2003-01-01

    The simple fact that proteins are built from 20 amino acids while DNA only contains four different bases, means that the 'signal-to-noise ratio' in protein sequence alignments is much better than in alignments of DNA. Besides this information-theoretical advantage, protein alignments also benefit...... proteins. It is therefore preferable to align coding DNA at the amino acid level and it is for this purpose we have constructed the program RevTrans. RevTrans constructs a multiple DNA alignment by: (i) translating the DNA; (ii) aligning the resulting peptide sequences; and (iii) building a multiple DNA...... alignment by 'reverse translation' of the aligned protein sequences. In the resulting DNA alignment, gaps occur in groups of three corresponding to entire codons, and analogous codon positions are therefore always lined up. These features are useful when constructing multiple DNA alignments for phylogenetic...

  13. Administrative circular No. 20 (Rev. 2) – Use of private vehicules for journeys on official duty

    CERN Multimedia

    HR Department

    2011-01-01

    Administrative Circular No. 20 (Rev. 2) entitled "Use of private vehicles for journeys on official duty", adopted following discussion in the Standing Concertation Committee meeting of 21 September 2010 and entering into force on 1 January 2011, is available on the intranet site of the Human Resources Department: http://cern.ch/hr-docs/admincirc/admincirc.asp It cancels and replaces Administrative Circular No. 20 (Rev. 1) entitled "Use of private vehicles on official duty" of April 1993. This new version introduces, in particular, the payment of a kilometer allowance in case of emergency during standby duty or when on call and a simplified calculation of the allowance for journeys between sites. This circular also clarifies the type of permitted private vehicles. Department Head Office  

  14. Administrative circular n°23 (Rev. 3) – Special working hours

    CERN Multimedia

    2013-01-01

    Administrative Circular No. 23 (Rev. 3) entitled “Special working hours”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 11 October 2012 and entering into force on 1 January 2013, is available on the intranet site of the Human Resources Department.   This circular is applicable to staff members and fellows. It cancels and replaces Administrative Circular No. 23 (Rev. 2) entitled “Special working hours” of December 2008. Paragraph 6 a) of Annex II of this circular was revised following the modification of Article III 1.04 of the Staff Regulations approved by Council on 14 December 2012. The modification serves to adapt the minimum rest time to the fact that, in case of rapidly alternating shifts, a maximum of seven consecutive shifts may be performed. Department Head Office HR Department

  15. Administrative circular n°11 (REV. 2) – Categories of members of the personnel

    CERN Multimedia

    2012-01-01

    Administrative Circular No. 11 (Rev. 2) entitled “Categories of members of the personnel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 11 May 2012 and entering into force on 1 January 2013, is available on the intranet site of the Human Resources Department. This circular is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 11 (Rev. 1) entitled “Categories of members of the personnel” of January 1997 as regards all contracts of members of the personnel issued on or after 1 January 2013. The circular was revised in order to take into account developments since the last revision of the categories of personnel in 1997 as well as the needs of the Organization and collaborating institutes. In particular, it introduces a new system for distinguishing categories of associated members of the personnel, namely with regard to the purpo...

  16. RevPAR、收益管理与收益管理经理

    Institute of Scientific and Technical Information of China (English)

    王大悟

    2013-01-01

    一、RevPAR这是酒店业熟悉的指标,即每间可供出租客房的实际平均房价,以客房门市价与出租率的乘积得出。这个指标至少考量了出租率与房价两个重要因素的组合,单纯出租率和房价并不能科学反映酒店的经营业绩。因此到目前为止,RevPAR依然是衡量与比较酒店经营状况的一个标准。

  17. Effects on birds of an offshore wind park at Horns Rev: Environmental impact assessment

    Energy Technology Data Exchange (ETDEWEB)

    Noer, H.; Kjaer Christensen, T.; Clausager, I.; Krag Petersen, I. [DMU, Dept. of Coastal Zone Ecology (Denmark)

    2000-07-01

    This report presents the technical background to the ornithological environmental impact assessment for the construction of an offshore windpark at Horns Rev, 14 km west-south-west of Blaevandshuk, Denmark. Construction of the park is planned to commence in 2001. The park will consist of c. 80 wind turbines, each of at least 1.8 MW, and cover an area of 27.5 km{sup 2} (including the 200 m exclusion zone around the park). (au)

  18. Hard bottom substrate monitoring Horns Rev offshore wind farm. Annual status report. 2004

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S.B.; Pedersen, John

    2005-05-15

    Elsam and Eltra have built the offshore demonstration wind farm at Horns Rev in the North Sea. Elsam is the owner and is responsible for the operation of the wind farm. Eltra is responsible for the connection of the wind farm to the national onshore grid. In the summer months of 2002, Elsam constructed the world's largest offshore wind farm at the Danish west coast. The wind farm is located 14-20 km into the North Sea, west of Blaevands Huk. The first wind turbine foundation was in place in March 2002 and the last mono-pile was in place in August 2002 for a total of 80. The construction work was completed with the last connecting cables sluiced down in September 2002. All the wind turbines were in production in December 2002. The expected impact from the wind farm will primarily be an alternation of habitats due to the introduction of hard bottom substrates as wind mono-piles and scour protections. A continuous development in the epifouling communities will be expected together with an introduction of new or alien species in the area. The indigenous benthic community in the area of Horn Rev can be characterised by infauna species belonging to the Goniadella-Spisula community. This community is typical of sandbanks in the North Sea area, although communities in such areas are very variable and site specific. Character species used as indicators for environmental changes in the Horns Rev area are the bristle worms Goniadella bobretzkii, Ophelia borealis, Psione remota and Orbinia sertulata and the mussels Goodallia triangularis and Spisula solida. In connection with the implementation of the monitoring programme concerning the ecological impact of the introduction of hard substrate related to the Horns Rev Wind Farm, surveys on hard bottom substrates were initialised in March 2003 with monitoring conducted in September 2003 and March and September 2004. This report describes the results from surveys on hard substrates in 2004. (au)

  19. International AIDS Society conference update. Compound targeting Rev protein promising. Orphaned approach still looking for home.

    Science.gov (United States)

    2005-09-01

    While clinicians and HIV/AIDS patients anxiously watch the trend of the virus developing resistance to multiple antiretroviral therapies, the question remains whether new drug research will continue to save the day. Some suggest there will need to be multiple new classes of antiretroviral drugs developed in order to stretch further the life span of longtime HIV patients. One potential new class would target the Rev protein, an approach that has received very little attention from the research and pharmaceutical communities.

  20. Detection of neutralizing antibodies to erythropoietin by inhibition of rHuEPO-stimulated EGR1 gene expression in the UT-7/EPO cell line.

    Science.gov (United States)

    Ferguson, Jackie; Bird, Chris; Wadhwa, Meenu; Burns, Chris

    2013-01-31

    Recombinant erythropoietin (rHuEPO) is used extensively to treat anaemia associated with chronic kidney disease. However, the development of neutralizing antibodies (NAbs) to rHuEPO can result in the development of antibody-mediated pure red cell aplasia (PRCA). The detection of NAb in patient sera by in vitro bioassay relies on the inhibition of a cellular response to rHuEPO. Current bioassays for rHuEPO measure proliferation in responsive cell lines such as the erythroleukaemic cell lines, UT-7 and UT-7/EPO, the latter sensitized to EPO. Using these cell lines, we show the dose-responsive induction of both PIM1 and EGR1 gene expression in UT-7 cells and of EGR1 in UT-7/EPO cells. The expression of EGR1 in UT-7/EPO cells in response to rHuEPO was comparable to the proliferative response measured by (3)H-thymidine incorporation and could be inhibited by serum from a patient with NAb-mediated PRCA in a dilution-dependent manner. Bioassays based on the induction of endogenous gene expression are comparable to current bioassays but are considerably quicker given that incubation time is decreased from 2-3 days to 50 min. Measurement of EGR1 gene expression in response to rHuEPO in UT-7/EPO cells offers a rapid, non-radioactive and automatable alternative to current assays for the detection of rHuEPO NAbs.

  1. 生物钟基因Rev-erb-α缺失导致雌鼠生理周期延长%Circadian clock gene Rev-erb-α deficiency prolongs female estrous cyclicity

    Institute of Scientific and Technical Information of China (English)

    张芹; 董珍; 吴曦; 颜洁; 高基民

    2013-01-01

    在哺乳动物中,生物节律与生殖生理和生殖内分泌密切相关,流行病学研究显示长期倒时差的女性表现出生殖力低下和不良妊娠的概率更高,但是人们对生物节律影响生殖的机制了解还很少.本研究旨在以时钟基因Rev-erb-α敲除型(Reverb-α-/-)雌性小鼠为模型初步探索生物节律对生殖的影响.将Rev-erb-α-/-和野生型(Rev-erb-α+/+)小鼠与C57BL/6J小鼠交配观察生殖力变化,用苏木精-伊红(HE)染色法观察卵巢组织变化,将Rev-erb-α+/+与Rev-erb-α-/-雌性小鼠分别与C57BL/6J雄性小鼠交配后比较见栓后1.5 d统计的胚胎数量,用阴道涂片结合周期图和周期谱观察小鼠生理周期变化.结果显示,Rev-erb-α-/-雌性小鼠与C57BL/6J雄鼠交配是不生育的,并且Rev-erb-α-/-雌性小鼠生成的胚胎数量明显减少.Rev-erb-α-/-雌性小鼠卵巢存在各级卵泡,并且结构与野生型(Rev-erb-α+/+)雌性小鼠相比无明显差异.Rev-erb-α-/-雌性小鼠的生理周期较Rev-erb-α+/+小鼠周期长约3.22 d,较Rev-erb-α+/+雌性小鼠延长63.5%.以上结果提示,生物钟基因Rev-erb-α缺失导致雌性小鼠生理周期明显延长,可能是影响雌性小鼠生育的原因之一.因此,生物节律的稳定对于生理周期具有重要作用.%The circadian clock has been linked to female reproductive physiology and endocrine in mammals.Epidemiological studies of female shift workers have shown increased rates of abnormal reproduction and adverse pregnancy.But little is known how the circadian rhythms affect reproduction.The aim of the present study was to investigate the influences of circadian rhythms on estrous cycle in female mice using clock gene Rev-erb-α knock out (Rev-erb-α-/-) mice.To test the fertility of Rev-erb-α-/-mice,litter sizes were counted after mating with C57BL/6J male mice.HE staining was used to observe the change of follicle development.The number of embryos of Rev-erb-α+/+ and Rev

  2. Losers, Food, and Sex. Clerical Masculinity in the BBC Sitcom Rev.

    Directory of Open Access Journals (Sweden)

    Alexander Ornella

    2016-11-01

    Full Text Available Clerical masculinities, much like their lay/secular counterparts, often appear unchang¬ing because they are the products of naturalization processes. Clerical masculinities, however, are far from stable, for they live and breathe the dynamics of both their socio-religious context and their secular “others”. The BBC sitcom Rev. (BBC2, UK 2010–2014 is a refreshing take on the everyday life and problems of a vicar in the Church of England trying to avoid stereotypes that often come with clerical roles. Rev. (2010–2014 can be interpreted as an attempt to explore the negotiation processes of masculinity within an institution that is involved in the “production” of religion and gender roles. It shows that being a man in an institutional setting is as much a perfor¬mance as it is a more or less successful negotiation of other people’s expectations and one’s own worldview. In particular, the main male clerical characters in Rev. (2010– 2014 inhabit a position of power but all have their flaws. They can best be understood as losers whose clash with masculine systems renders them more human.

  3. Testing Hu-Sawicki f(R) gravity with the Effective Field Theory approach

    CERN Document Server

    Hu, Bin; Rizzato, Matteo; Silvestri, Alessandra

    2016-01-01

    We show how to fully map a specific model of modified gravity into the Einstein-Boltzmann solver EFTCAMB. This approach consists in few steps and allows to obtain the cosmological phenomenology of a model with minimal effort. We discuss all these steps, from the solution of the dynamical equations for the cosmological background of the model to the use of the mapping relations to cast the model into the effective field theory language and use the latter to solve for perturbations. We choose the Hu-Sawicki f(R) model of gravity as our working example. After solving the background and performing the mapping, we interface the algorithm with EFTCAMB and take advantage of the effective field theory framework to integrate the full dynamics of linear perturbations, returning all quantities needed to accurately compare the model with observations. We discuss some observational signatures of this model, focusing on the linear growth of cosmic structures. In particular we present the behavior of $f\\sigma_8$ and $E_G$ t...

  4. La huída en la obra de Mario Vargas Llosa

    Directory of Open Access Journals (Sweden)

    German Arce Ross

    Full Text Available Se trata de poner en perspectiva la mayor parte de novelas de Mario Vargas Llosa en función del tema de la huída, partiendo del hecho que é1 solicitó la nacionalidad Española inmediatamente después de haber perdido las elecciones para la Presidencia del Perú y de haber escrito El pez en el agua. Nuestro análisis intenta demostrar la importancia de la figura paterna – principalmente en el sentido de su ausencia y de sus bruscas reapariciones – en la cristalización sintomática del tema de la fuga. Es por eso que una analogía con la posición fóbica, en relación al padre o a sus substitutos, nos ha parecido pertinente. Teniendo como pivot el análisis de la forma, del contexto y del contenido de su libro de memorias, este trabajo definiría así las bases de una contribución literaria de la obra de Vargas Llosa a la clinica de la fobia.

  5. Systematics of a kleptoplastidal dinoflagellate, Gymnodinium eucyaneum Hu (Dinophyceae, and its cryptomonad endosymbiont.

    Directory of Open Access Journals (Sweden)

    Shuang Xia

    Full Text Available New specimens of the kleptoplastidal dinoflagellate Gymnodinium eucyaneum Hu were collected in China. We investigated the systematics of the dinoflagellate and the origin of its endosymbiont based on light morphology and phylogenetic analyses using multiple DNA sequences. Cells were dorsoventrally flattened with a sharply acute hypocone and a hemispherical epicone. The confusion between G. eucyaneum and G. acidotum Nygaard still needs to be resolved. We found that the hypocone was conspicuously larger than the epicone in most G. eucyaneum cells, which differed from G. acidotum, but there were a few cells whose hypocone and epicone were of nearly the same size. In addition, there was only one site difference in the partial nuclear LSU rDNA sequences of a sample from Japan given the name G. acidotum and G. eucyaneum in the present study, which suggest that G. eucyaneum may be a synonym of G. acidotum. Spectroscopic analyses and phylogenetic analyses based on nucleomorph SSU rDNA sequences and chloroplast 23 s rDNA sequences suggested that the endosymbiont of G. eucyaneum was derived from Chroomonas (Cryptophyta, and that it was most closely related to C. coerulea Skuja. Moreover, the newly reported kleptoplastidal dinoflagellates G. myriopyrenoides and G. eucyaneum in our study were very similar, and the taxonomy of kleptoplastidal dinoflagellates was discussed.

  6. Screening of a xylanase clone from a fosmid library of rumen microbiota in Hu sheep.

    Science.gov (United States)

    Wang, Jiakun; Sun, Zhongyuan; Zhou, Yan; Wang, Qian; Ye, Jun'an; Chen, Zhenming; Liu, Jianxin

    2012-01-01

    The glycosyl hydrolase family 11, which is responsible for carbohydrate metabolism, was identified in the open reading frame (ORF) 6 of a xylanase positive clone from a fosmid library of rumen microbiota of Hu sheep. A BLASTP search of GenBank revealed that ORF6 encoded a 355-amino acid putative endoxylanase, having 61% similarity (e(-73)) to endo-1,4-β-xylanase of Fibrobacter succinogenes S85 (YP_003250510.1). Predicted with the SWISS-MODEL, there were two separate β-sandwich clusters linked with a high serine containing linker in ORF6. The N-terminal β-sandwich is a novel endoxylanase of the glycosyl hydrolase family 11 with a specific activity of 1150.00 U/mg. The optimal pH and temperature for this enzyme were shown to be pH 5.0 and 50°C, respectively. The C-terminal helped increase the stability of the xylanase but decreased the activity to some degree. The C-terminal β-sandwich could bind avicel, but no conserved domain could be found. It may be a novel carbohydrate-binding module.

  7. Managing Stability in the Taiwan Strait: Non-Military Policy towards Taiwan under Hu Jintao

    Directory of Open Access Journals (Sweden)

    Wu-ueh Chang

    2009-10-01

    Full Text Available China’s Taiwan policy has been one of coupling intimidation (the “stick” approach with coercion (the “carrot” approach, a policy mix which, in the near term, is not likely to change, as is evidenced by the passage of the “Anti-Secession Law” in March, 2005. However, under Hu Jintao, the focus has been on pragmatism. The warm atmosphere that presently reigns in the Taiwan Strait area is unprecedented. Further talks are expected before the two cross-Strait leaders are slated to step down, simultaneously, in 2012. An era of reconciliation and negotiations has dawned. For the first time there is consensus regarding norms of interaction between the two sides. Cross-Strait relations have stabilized after years of tumult. More open, stable and predictable cross-Strait relations are in the interests of both sides. Difficulties surely lie ahead, but they will be dealt with in a different manner than what has been witnessed in the past.

  8. Detection of a planetary system orbiting the eclipsing polar HU Aqr

    CERN Document Server

    Qian, S -B; Liao, W -P; Li, L -J; Zhu, L -Y; Dai, Z -B; He, J -J; Zhao, E -G; Zhang, J; Li, K

    2011-01-01

    Using the precise times of mid-egress of the eclipsing polar HU Aqr, we discovered that this polar is orbited by two or more giant planets. The two planets detected so far have masses of at least 5.9 and 4.5\\,M_{Jup}. Their respective distances from the polar are 3.6 AU and 5.4 AU with periods of 6.54 and 11.96 years, respectively. The observed rate of period decrease derived from the downward parabolic change in O-C curve is a factor 15 larger than the value expected for gravitational radiation. This indicates that it may be only a part of a long-period cyclic variation, revealing the presence of one more planet. It is interesting to note that the two detected circumbinary planets follow the Titus-Bode law of solar planets with n=5 and 6. We estimate that another 10 years of observations will reveal the presence of the predicted third planet.

  9. Construction of a eukaryotic expression plasmid pcDNA3.1-HuR-FLAG and its transient expression in NIH3T3 cells

    Directory of Open Access Journals (Sweden)

    Tao LI

    2011-04-01

    Full Text Available Objective To construct a eukaryotic expression vector for HuR and analyze its expression and biological function in NIH3T3 cells.Methods The total RNA was extracted from NIH3T3 cells and reverse transcribed to cDNAs.The coding region sequence of mouse HuR was then amplified by PCR and subcloned into the pcDNA3.1-FLAG plasmid.The recombinant plasmid pcDNA3.1-HuR-FLAG was verified by PCR and restriction endonuclease analysis,confirmed by DNA sequence analysis,and then transiently transfected into NIH3T3 cells with Lipofectamine LTX.The expression of HuR protein was determined by Western blotting,and the mRNA level of HuR and DUSP1 were analyzed by using real-time PCR.Result The recombinant plasmid pcDNA3.1-HuR-FLAG was correctly constructed.Twenty-four hours after transfection of the recombinant plasmid into NIH3T3 cells,the fusion protein was found to have highly expressed in the cells as revealed by Western blotting.Real-time PCR results detected that the over-expression of HuR could up-regulate the expression of DUSP1.Conclusion The eukaryotic expression vector for HuR-FLAG fusion protein has been successfully constructed and transiently expressed in NIH3T3 cells.It can be used in further analysis of the posttranscriptional regulation of DUSP1 by HuR in cancer cells.

  10. Massive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips.

    Energy Technology Data Exchange (ETDEWEB)

    Krylov, A. S.; Zasedateleva, O. A.; Prokopenko, D. V.; Rouviere-Yaniv, J.; Mirzabekov, A. D.; Biochip Technology Center; Engelhardt Inst. of Molecular Biology; Inst. de Biologie Physico-Chimique

    2001-06-15

    A generic hexadeoxyribonucleotide microchip has been applied to test the DNA-binding properties of HU histone-like bacterial protein, which is known to have a low sequence specificity. All 4096 hexamers flanked within 8mers by degenerate bases at both the 3'- and 5'-ends were immobilized within the 100 x 100 x 20 mm polyacrylamide gel pads of the microchip. Single-stranded immobilized oligonucleotides were converted in some experiments to the double-stranded form by hybridization with a specified mixture of 8mers. The DNA interaction with HU was characterized by three type of measurements: (i) binding of FITC-labeled HU to microchip oligonucleotides; (ii) melting curves of complexes of labeled HU with single-stranded microchip oligonucleotides; (iii) the effect of HU binding on melting curves of microchip double-stranded DNA labeled with another fluorescent dye, Texas Red. Large numbers of measurements of these parameters were carried out in parallel for all or many generic microchip elements in real time with a multi-wavelength fluorescence microscope. Statistical analysis of these data suggests some preference for HU binding to G/C-rich single-stranded oligonucleotides. HU complexes with double-stranded microchip 8mers can be divided into two groups in which HU binding either increased the melting temperature (T{sub m}) of duplexes or decreased it. The stabilized duplexes showed some preference for presence of the sequence motifs AAG, AGA and AAGA. In the second type of complex, enriched with A/T base pairs, the destabilization effect was higher for longer stretches of A/T duplexes. Binding of HU to labeled duplexes in the second type of complex caused some decrease in fluorescence. This decrease also correlates with the higher A/T content and lower T{sub m}. The results demonstrate that generic microchips could be an efficient approach in analysis of sequence specificity of proteins.

  11. Antidepressant effects of curcumin and HU-211 coencapsulated solid lipid nanoparticles against corticosterone-induced cellular and animal models of major depression

    Directory of Open Access Journals (Sweden)

    He XL

    2016-10-01

    Full Text Available Xiaolie He,* Yanjing Zhu,* Mei Wang, Guoxin Jing, Rongrong Zhu, Shilong Wang Research Center for Translational Medicine at East Hospital, School of Life Science and Technology, Tongji University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Major depression is a complex neuropsychiatric disorder with few treatment approaches. The use of nontargeted antidepressants induced many side effects with their low efficacy. A more precise targeting strategy is to develop nanotechnology-based drug delivery systems; hence, we employed solid lipid nanoparticles (SLNs to encapsulate HU-211 and curcumin (Cur. The antidepressant effects of the dual-drug nanoparticles (Cur/SLNs-HU-211 for major depression treatment were investigated in corticosterone-induced cellular and animal models of major depression. Cur/SLNs-HU-211 can effectively protect PC12 cells from corticosterone-induced apoptosis and can release more dopamine, which may be associated with the higher uptake of Cur/SLNs-HU-211 shown by cellular uptake behavior analysis. Additionally, Cur/SLNs-HU-211 significantly reduced the immobility time in forced swim test, enhanced fall latency in rotarod test, and improved the level of dopamine in mice blood. Cur/SLNs-HU-211 can deliver more Cur to the brain and thus produce a significant increase in neurotransmitters level in brain tissue, especially in the hippocampus and striatum. The results of Western blot and immunofluorescence revealed that Cur/SLNs-HU-211 can significantly enhance the expression of CB1, p-MEK1, and p-ERK1/2. Our study suggests that Cur/SLNs-HU-211 may have great potential for major depression treatment. Keywords: major depression, curcumin, HU-211, solid lipid nanoparticles, dopamine

  12. Design and Implementation of 3D Dressing System Based on Physics Engine PhysX%基于物理引擎PhysX的3D试衣系统的设计与实现

    Institute of Scientific and Technical Information of China (English)

    徐康熙; 郝泳涛

    2014-01-01

    In order to achieve the 3D fitting function and solve the problem of large algorithm time complexity of virtual try modeling, based on virtual reality technology, this paper tend to model clothing fabric by sprint-mass model. Then, based on Newton's second law, we analysis the spring-mass model-based forces of clothing fabric modeling. Combined with GPU-based physics engine PhysX, build the 3D fitting room, simulate the sports interactive situation of fabrics and mannequin. Finally add mannequin adjustment, the election of clothing, dressing and other functions. Built under Windows platform, designed and devel-oped by using VisualStudio2008, combined with the comprehensive application of virtual reality technology, we establish the 3D fitting system, including functions such as clothing select, dressing, body parameter adjustment .%为了实现3D试衣功能及解决虚拟试衣建模算法时间复杂度比较大的问题,该文结合虚拟现实等技术,首先用弹簧-质点模型对服装布料进行建模,然后以牛顿第二定律为理论基础分析以质点-弹簧模型建模的服装布料的受力情况,结合以GPU为基础的物理引擎PhysX,建立3D试衣间,仿真服装布料与人体模型的运动交互状况。最后加入人体模型调整、选衣、试衣等功能。该系统在Windows平台下,利用VisualStudio2008设计开发,结合虚拟现实等技术的综合应用,建立了一套包括选衣、试衣、人体参数调整等功能的3D试衣系统。

  13. Effect of various factors and substrates on the growth of a human hepatoblastoma cell line, HuH-6 in a serum-free medium.

    Directory of Open Access Journals (Sweden)

    Tokiwa,Takayoshi

    1989-12-01

    Full Text Available The effect of various factors and substrates on the growth of a human hepatoblastoma cell line, HuH-6, which was inoculated at low density in a serum-free medium was examined. Several supplements were required to enhance cell growth of HuH-6. These included cholera toxin (CT, glucagon (Glu and selenium (Se. Type IV collagen (C-IV provided the most conductive environment tested for cell growth. These results suggest that CT, Glu, Se, and C-IV are important stimulators for the continuous growth of HuH-6 in a serum-free medium at low density.

  14. Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function

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    Myers Michael P

    2011-07-01

    Full Text Available Abstract Background Central to the fully competent replication cycle of the human immunodeficiency virus type 1 (HIV-1 is the nuclear export of unspliced and partially spliced RNAs mediated by the Rev posttranscriptional activator and the Rev response element (RRE. Results Here, we introduce a novel method to explore the proteome associated with the nuclear HIV-1 RNAs. At the core of the method is the generation of cell lines harboring an integrated provirus carrying RNA binding sites for the MS2 bacteriophage protein. Flag-tagged MS2 is then used for affinity purification of the viral RNA. By this approach we found that the viral RNA is associated with the host nuclear matrix component MATR3 (Matrin 3 and that its modulation affected Rev activity. Knockdown of MATR3 suppressed Rev/RRE function in the export of unspliced HIV-1 RNAs. However, MATR3 was able to associate with Rev only through the presence of RRE-containing viral RNA. Conclusions In this work, we exploited a novel proteomic method to identify MATR3 as a cellular cofactor of Rev activity. MATR3 binds viral RNA and is required for the Rev/RRE mediated nuclear export of unspliced HIV-1 RNAs.

  15. Identification of flavonoid diglycosides in several genotypes of asparagus from the Huétor-Tájar population variety.

    Science.gov (United States)

    Fuentes-Alventosa, José María; Rodríguez, Guillermo; Cermeño, Pedro; Jiménez, Ana; Guillén, Rafael; Fernández-Bolaños, Juan; Rodríguez-Arcos, Rocío

    2007-11-28

    The qualitative and quantitative composition of flavonoids from the Huétor-Tájar population variety of asparagus (commonly known as " triguero") was investigated. Flavonoids were analyzed by reversed-phase high-performance liquid chromatography-diode array detection (HPLC-DAD). Liquid chromatography-mass spectrometry (LC-MS) under identical HPLC conditions was used to verify the identities of the flavonoid glycosides from triguero asparagus. The quantities of asparagus flavonoids were calculated according to concentration curves constructed with authentic standards. Total flavonoid contents, calculated as the sum of individual compounds, were determined and ranged from 400 to 700 mg/kg fresh weight. The most abundant was rutin, which represented 55-98% of the total flavonoid complement. Triguero asparagus were revealed to be an important source of not only quercetin derivatives but also kaempferol and isorhamnetin glycosides. Significant differences (p asparagus genotypes from the Huétor-Tájar population variety.

  16. Status report of seabird surveys at Horns Rev, 2000-2001

    Energy Technology Data Exchange (ETDEWEB)

    Kjaer Christensen, T.; Clausager, I.; Krag Petersen, I. [NERI, Dept. of Coastal Zone Ecology, Roskilde (Denmark)

    2002-07-01

    The present report presents the results of three bird surveys conducted in the Horns Rev area during the second half of 2001. Due to poor weather conditions in December 2001, the last survey was, however, performed on 7 January 2002. The surveys are part of the base-line investigations of birds performed in relation to the proposed construction of an offshore wind farm at Horns Rev in the Danish part of the North Sea ca 14 km southwest of Blaevandshuk. The results of the surveys during August 2001 - January 2002 are presented together with the results obtained during the period August 2000 - April 2001, and are also compared to results obtained during the period August 1999 - April 2000. Based on the distribution of the most abundant bird species recorded during 16 aerial surveys performed during August 1999 - January 2002, there were no indications that the wind farm area was of any particular importance to the birds' exploitation of the Horns Rev area. Fish-eating species like divers, gannet, terns, auks and gulls generally showed scattered and variable distributions, mainly occurring in the areas north and south of Horns Rev, and with low numbers on the reef proper and within the planned wind farm area. The distribution of benthic foraging species, eider and common Scoter, showed that they mainly exploited the coastal parts of the area off Blaevandshuk and Skallingen, although common scoter was found in relatively high numbers on the southeast slopes of the Horns Rev and within the wind farm area in the April 2001 survey. Common scoters occurred in very high numbers in January 2002. This was probably related to increased immigration of birds from the inner Danish waters during a cold period in late December 2001. Preference analyses of bird exploitation of the Horns Rev area showed that if the birds completely avoid the wind farm area after erection of the wind turbines, this will affect less than 1% of the various species, except divers where 1.58% will be

  17. HTLV-1 Infection and Neuropathogenesis in the Context of Rag1(-/-)γc(-/-) (RAG1-Hu) and BLT Mice.

    Science.gov (United States)

    Ginwala, Rashida; Caruso, Breanna; Khan, Zafar K; Pattekar, Ajinkya; Chew, Glen M; Corley, Michael J; Loonawat, Ronak; Jacobson, Steven; Sreedhar, Sreesha; Ndhlovu, Lishomwa C; Jain, Pooja

    2017-09-01

    To date, the lack of a suitable small animal model has hindered our understanding of Human T-cell lymphotropic virus (HTLV)-1 chronic infection and associated neuropathogenesis defined as HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The host immune response plays a critical role in the outcome of HTLV-1 infection, which could be better tested in the context of humanized (hu) mice. Thus, we employ here the Balb/c-Rag1(-/-)γc(-/-) or Rag1 as well as Bone marrow-Liver-Thymic (BLT) mouse models for engraftment of human CD34(+) hematopoietic stem cells. Flow cytometry and histological analyses confirmed reconstitution of Rag1 and BLT mice with human immune cells. Following HTLV-1 infection, proviral load (PVL) was detected in the blood of Rag-1 and BLT hu-mice as early as 2 weeks post-infection (wpi) with sustained elevation in the subsequent weeks followed by Tax expression. Additionally, infection was compared between adult and neonatal Rag1 mice with both PVL and Tax expression considerably higher in the adult Rag1 mice as compared to the neonates. Establishment of peripheral infection led to lymphocytic infiltration with concomitant Tax expression and resulting myelin disruption within the central nervous system of infected mice. In addition, up-regulation in the expression of several immune checkpoint mediators such as programmed cell death-1 (PD-1), T-cell Ig and ITIM domain (TIGIT), and T cell Ig and mucin domain-3 protein (Tim-3) were observed on CD8(+) T cells in various organs including the CNS of infected hu-mice. Collectively, these studies represent the first attempt to establish HTLV-1 neuropathogenesis in the context of Rag-1 and BLT hu-mice as potential novel tools for understanding HTLV-1 neuropathogenesis and testing of novel therapies such as immune checkpoint blockade in the amelioration of chronic HTLV-1 infection.

  18. Comment on "Tropospheric temperature response to stratospheric ozone recovery in the 21st century" by Hu et al. (2011

    Directory of Open Access Journals (Sweden)

    C. McLandress

    2012-03-01

    Full Text Available In a recent paper Hu et al. (2011 suggest that the recovery of stratospheric ozone during the first half of this century will significantly enhance free tropospheric and surface warming caused by the anthropogenic increase of greenhouse gases, with the effects being most pronounced in Northern Hemisphere middle and high latitudes. These surprising results are based on a multi-model analysis of CMIP3 model simulations with and without prescribed stratospheric ozone recovery. Hu et al. suggest that in order to properly quantify the tropospheric and surface temperature response to stratospheric ozone recovery, it is necessary to run coupled atmosphere-ocean climate models with stratospheric ozone chemistry. The results of such an experiment are presented here, using a state-of-the-art chemistry-climate model coupled to a three-dimensional ocean model. In contrast to Hu et al., we find a much smaller Northern Hemisphere tropospheric temperature response to ozone recovery, which is of opposite sign. We suggest that their result is an artifact of the incomplete removal of the large effect of greenhouse gas warming between the two different sets of models.

  19. Cannabinoid HU210 protects isolated rat stomach against impairment caused by serum of rats with experimental acute pancreatitis.

    Directory of Open Access Journals (Sweden)

    Ming-hua Cao

    Full Text Available Acute pancreatitis (AP, especially severe acute pancreatitis often causes extra-pancreatic complications, such as acute gastrointestinal mucosal lesion (AGML which is accompanied by a considerably high mortality, yet the pathogenesis of AP-induced AGML is still not fully understood. In this report, we investigated the alterations of serum components and gastric endocrine and exocrine functions in rats with experimental acute pancreatitis, and studied the possible contributions of these alterations in the pathogenesis of AGML. In addition, we explored the intervention effects of cannabinoid receptor agonist HU210 and antagonist AM251 on isolated and serum-perfused rat stomach. Our results showed that the AGML occurred after 5 h of AP replication, and the body homeostasis was disturbed in AP rat, with increased levels of pancreatic enzymes, lipopolysaccharide (LPS, proinflammtory cytokines and chemokines in the blood, and an imbalance of the gastric secretion function. Perfusing the isolated rat stomach with the AP rat serum caused morphological changes in the stomach, accompanied with a significant increment of pepsin and [H+] release, and increased gastrin and decreased somatostatin secretion. HU210 reversed the AP-serum-induced rat pathological alterations, including the reversal of transformation of the gastric morphology to certain degree. The results from this study prove that the inflammatory responses and the imbalance of the gastric secretion during the development of AP are responsible for the pathogenesis of AGML, and suggest the therapeutic potential of HU210 for AGML associated with acute pancreatitis.

  20. Alginate hydrogel protects encapsulated hepatic HuH-7 cells against hepatitis C virus and other viral infections.

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    Nhu-Mai Tran

    Full Text Available Cell microencapsulation in alginate hydrogel has shown interesting applications in regenerative medicine and the biomedical field through implantation of encapsulated tissue or for bioartificial organ development. Although alginate solution is known to have low antiviral activity, the same property regarding alginate gel has not yet been studied. The aim of this work is to investigate the potential protective effect of alginate encapsulation against hepatitis C virus (HCV infection for a hepatic cell line (HuH-7 normally permissive to the virus. Our results showed that alginate hydrogel protects HuH-7 cells against HCV when the supernatant was loaded with HCV. In addition, alginate hydrogel blocked HCV particle release out of the beads when the HuH-7 cells were previously infected and encapsulated. There was evidence of interaction between the molecules of alginate hydrogel and HCV, which was dose- and incubation time-dependent. The protective efficiency of alginate hydrogel towards HCV infection was confirmed against a variety of viruses, whether or not they were enveloped. This promising interaction between an alginate matrix and viruses, whose chemical mechanisms are discussed, is of great interest for further medical therapeutic applications based on tissue engineering.

  1. Chemopreventive apigenin controls UVB-induced cutaneous proliferation and angiogenesis through HuR and thrombospondin-1.

    Science.gov (United States)

    Tong, Xin; Mirzoeva, Salida; Veliceasa, Dorina; Bridgeman, Bryan B; Fitchev, Philip; Cornwell, Mona L; Crawford, Susan E; Pelling, Jill C; Volpert, Olga V

    2014-11-30

    Plant flavonoid apigenin prevents and inhibits UVB-induced carcinogenesis in the skin and has strong anti-proliferative and anti-angiogenic properties. Here we identify mechanisms, by which apigenin controls these oncogenic events. We show that apigenin acts, at least in part, via endogenous angiogenesis inhibitor, thrombospondin-1 (TSP1). TSP1 expression by the epidermal keratinocytes is potently inhibited by UVB. It inhibits cutaneous angiogenesis and UVB-induced carcinogenesis. We show that apigenin restores TSP1 in epidermal keratinocytes subjected to UVB and normalizes proliferation and angiogenesis in UVB-exposed skin. Importantly, reconstituting TSP1 anti-angiogenic function in UVB-irradiated skin with a short bioactive peptide mimetic representing exclusively its anti-angiogenic domain reproduced the anti-proliferative and anti-angiogenic effects of apigenin. Cox-2 and HIF-1α are important mediators of angiogenesis. Both apigenin and TSP1 peptide mimetic attenuated their induction by UVB. Finally we identified the molecular mechanism, whereby apigenin did not affect TSP1 mRNA, but increased de novo protein synthesis. Knockdown studies implicated the RNA-binding protein HuR, which controls mRNA stability and translation. Apigenin increased HuR cytoplasmic localization and physical association with TSP1 mRNA causing de novo TSP1 synthesis. HuR cytoplasmic localization was, in turn, dependent on CHK2 kinase. Together, our data provide a new mechanism, by which apigenin controls UVB-induced carcinogenesis.

  2. Cannabinoid HU210 Protects Isolated Rat Stomach against Impairment Caused by Serum of Rats with Experimental Acute Pancreatitis

    Science.gov (United States)

    Cao, Ming-hua; Li, Yong-yu; Xu, Jing; Feng, Ya-jing; Lin, Xu-hong; Li, Kun; Han, Tong; Chen, Chang-Jie

    2012-01-01

    Acute pancreatitis (AP), especially severe acute pancreatitis often causes extra-pancreatic complications, such as acute gastrointestinal mucosal lesion (AGML) which is accompanied by a considerably high mortality, yet the pathogenesis of AP-induced AGML is still not fully understood. In this report, we investigated the alterations of serum components and gastric endocrine and exocrine functions in rats with experimental acute pancreatitis, and studied the possible contributions of these alterations in the pathogenesis of AGML. In addition, we explored the intervention effects of cannabinoid receptor agonist HU210 and antagonist AM251 on isolated and serum-perfused rat stomach. Our results showed that the AGML occurred after 5 h of AP replication, and the body homeostasis was disturbed in AP rat, with increased levels of pancreatic enzymes, lipopolysaccharide (LPS), proinflammtory cytokines and chemokines in the blood, and an imbalance of the gastric secretion function. Perfusing the isolated rat stomach with the AP rat serum caused morphological changes in the stomach, accompanied with a significant increment of pepsin and [H+] release, and increased gastrin and decreased somatostatin secretion. HU210 reversed the AP-serum-induced rat pathological alterations, including the reversal of transformation of the gastric morphology to certain degree. The results from this study prove that the inflammatory responses and the imbalance of the gastric secretion during the development of AP are responsible for the pathogenesis of AGML, and suggest the therapeutic potential of HU210 for AGML associated with acute pancreatitis. PMID:23285225

  3. Effect of route of infection on outcome of Toxoplasma gondii infection in hu-PBL SCID mice.

    Science.gov (United States)

    Meyer, Desiree J; Allan, Jane E; Beaman, Miles H

    2013-03-01

    Toxoplasma gondii (T. gondii) causes serious infection, especially in immunocompromised hosts. The relevance of animal models of toxoplasmosis to human disease is unclear, but have indicated that the route of Toxoplasma infection may affect the outcome. A humanized model of toxoplasmosis of immunocompromised mice (i.e. hu-PBL SCID), using the intraperitoneal (IP) route demonstrated long-term engraftment of human cells and worsening of inflammation compared to controls. In this study, we examined the effect of route of infection on this hu-PBL SCID model using a Toxoplasma strain (i.e. DAG) isolated from an immunocompromised human. Oral infection led to an asymptomatic infection, whereas animals infected by the IP route succumbed more quickly to infection. Human cells, detected through species-specific β-actin mRNA, were not as prominent in IP-infected animals as compared to orally infected and uninfected animals. There was evidence of control of toxoplasmosis in some orally infected animals, and this was associated with the presence of human cells in multiple tissues. Thus, the route of infection dramatically affects the outcome of infection, either by affecting parasite replication or expansion of human immune cells. Further studies of oral Toxoplasma infection using hu-PBL SCID mice may help in developing chemotherapies and immunotherapeutic strategies for toxoplasmosis.

  4. A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

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    Zhaoyang Zhang

    2011-11-01

    Full Text Available We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α. In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor, on the surface of which the complementary sequences are linked (as cODN-AuNPs and pre-hybridized with carboxymethylfluorescein (FAM-labeled anti-rHuEPO-α aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-α concentrations as low as 0.92 nM within a few hours.

  5. Comment on "Tropospheric temperature response to stratospheric ozone recovery in the 21st century" by Hu et al. (2011

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    M. Previdi

    2012-01-01

    Full Text Available Stratospheric ozone recovery is expected to figure prominently in twenty-first century climate change. In a recent paper, Hu et al. (2011 argue that one impact of ozone recovery will be to enhance the warming of the surface-troposphere system produced by increases in well-mixed greenhouse gases; furthermore, this enhanced warming would be strongest in the Northern Hemisphere, which is surprising since previous studies have consistently shown the effects of stratospheric ozone changes to be most pronounced in the Southern Hemisphere. Hu et al. (2011 base their claims largely on differences in the simulated temperature change between two groups of IPCC climate models, one group which included stratospheric ozone recovery in its twenty-first century simulations and a second group which did not. Both groups of models were forced with the same increases in well-mixed greenhouse gases according to the A1B emissions scenario. In the current work, we compare the surface temperature responses of the same two groups of models in a different experiment in which atmospheric CO2 was increased by 1% per year until doubling. We find remarkably similar differences in the simulated surface temperature change between the two sets of models as Hu et al. (2011 found for the A1B experiment, suggesting that the enhanced warming which they attribute to stratospheric ozone recovery is actually a reflection of different responses of the two model groups to greenhouse gas forcing.

  6. Morphological and kinematic signatures of a binary central star in the planetary nebula Hu 2-1

    CERN Document Server

    Miranda, L F; Guerrero, M; Vázquez, R A; Gómez, Y; Miranda, Luis F.; Torrelles, Jose M.; Guerrero, Martin A.; Vazquez, Roberto; Gomez, Yolanda

    2000-01-01

    We present narrow-band optical images, VLA-A 3.6 cm continuum and H92alpha emission line data and high resolution long-slit [N II] spectra of Hu 2-1. The formation of Hu 2-1 appears dominated by anisotropic mass ejection during the late AGB stage of its progenitor and by variable, ``precessing'' collimated bipolar outflows during the proto-PN and/or early PN phases. The data strongly support the existence of a binary central star at center of Hu 2-1: (1) the brightness distribution of the bipolar lobes and inner shell is point-symmetric, and the bipolar lobes show departures from axial symmetry; (2) the position of the central star is off-center; (3) mass ejection towards the equatorial plane is detected; (4) ``precessing'' collimated outflows are present. Besides all this circumstantial evidences, the analysis of the kinematics shows that the systemic velocity of the bipolar outflows does not coincide with the systemic velocity of the nebula. We propose that this velocity difference is a direct evidence of o...

  7. Modeling and predicting hemorrhagic fever with renal syndrome trends based on meteorological factors in Hu County, China.

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    Dan Xiao

    Full Text Available Hu County is a serious hemorrhagic fever with renal syndrome (HFRS epidemic area, with notable fluctuation of the HFRS epidemic in recent years. This study aimed to explore the optimal model for HFRS epidemic prediction in Hu.Three models were constructed and compared, including a generalized linear model (GLM, a generalized additive model (GAM, and a principal components regression model (PCRM. The fitting and predictive adjusted R2 of each model were calculated. Ljung-Box Q tests for fitted and predicted residuals of each model were conducted. The study period was stratified into before (1971-1993 and after (1994-2012 vaccine implementation epochs to avoid the confounding factor of vaccination.The autocorrelation of fitted and predicted residuals of the GAM in the two epochs were not significant (Ljung-Box Q test, P>.05. The adjusted R2 for the predictive abilities of the GLM, GAM, and PCRM were 0.752, 0.799, and 0.665 in the early epoch, and 0.669, 0.756, and 0.574 in the recent epoch. The adjusted R2 values of the three models were lower in the early epoch than in the recent epoch.GAM is superior to GLM and PCRM for monthly HFRS case number prediction in Hu County. A shift in model reliability coincident with vaccination implementation demonstrates the importance of vaccination in HFRS control and prevention.

  8. Orally administrated Juzen-taiho-to/TJ-48 ameliorates erythropoietin (rHuEPO)-resistant anemia in patients on hemodialysis.

    Science.gov (United States)

    Nakamoto, Hidetomo; Mimura, Taku; Honda, Nobuko

    2008-10-01

    Maintenance of the red blood cell volume is a fundamental aspect of ensuring oxygen supply to the tissue. Recombinant human erythropoietin (rHuEPO) was approved for marketing in Japan in 1990 for the treatment of anemia in patients on dialysis. Recombinant human erythropoietin caused a significant increase in hemoglobin (Hb) levels in patients on dialysis. However, not all have a good response to rHuEPO therapy; the causes of rHuEPO failure include iron deficiency, infection, uremia, and interaction of some drugs. Juzen-taiho-to (TJ-48), a mixture of extracts from 10 medicinal herbs, has been used traditionally to treat patients with anemia, anorexia, or fatigue. To clarify the effect of TJ-48 on erythropoietin-resistant anemia, we studied the effect of TJ-48 in patients on hemodialysis with erythropoietin-resistant anemia. We divided 42 end-stage renal disease patients on hemodialysis with erythropoietin-resistant anemia (Hbrenal disease patients. This effect was, at least in part, due to the anti-inflammatory effect of TJ-48 in patients on hemodialysis.

  9. Bone and muscle assessment in patients undergoing total hip arthroplasty using HU based analysis

    Directory of Open Access Journals (Sweden)

    Þröstur Pétursson

    2012-09-01

    Full Text Available Total hip arthroplasty (THA is performed with or without the use of bone cement. The lack of reliable clinical guidelines for deciding which one to implement has encouraged this approach of joint clinical and engineering with the following objectives: 1. Validate quadriceps muscles and femur bone atrophy by extracting the mineral density from Computer Tomographic (CT images. 2. Validate computational processes based on 3-D modeling and Finite Element Methods (FEM. A clinical trial was started, where 36 volunteer patients underwent THA surgery for the first time: 18 receiving cemented implant and 18 receiving uncemented implant. The patients were CT scanned prior-, immediately after and 12 months post-surgery. The CT data are further processed to segment muscles and bones and to create 3D-models for the simulation and for calculating bone mineral density (BMD. Furthermore quadriceps muscle density Hounsfield (HU based value is calculated from the segmented file on healthy and operated leg. These preliminary results indicate computational tools and methods that are able to quantitatively analyse patient’s condition pre and post-surgery. The BMD and muscle density measurement in correlation with the fracture risk analysis display a potential method for eligibility to receive non-cemented implant; the preliminary results show that also elderly that according with current clinical evaluation receives a cemented implant are suitable for the non-cemented type. The risk for structural failure during THA surgery is estimated by calculating femoral bone fracture risk index (FRI as a ratio between compressive stress during surgery and estimated failure stress on bone. The correlations with the BMD observations during the clinical trial will assess and validate this potential predictor tool.

  10. Publicidad y consumo de alimentos en estudiantes de Huánuco (Perú

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    María Luisa Sevillano García

    2012-10-01

    Full Text Available Las importantes carencias nutritivas apreciadas en estudiantes del distrito de Huánuco (Perú, junto con la publicidad televisiva de productos de dudosa calidad nutricional observada, nos llevó a plantear y desarrollar una investigación con el objeto de analizar la relación entre ambos fenómenos titulada: Influencia de los anuncios televisivos en el consumo de alimentos publicitados durante el 2010, cuyo resumen se presenta en este artículo. En la investigación planteamos un estudio correlacional con una muestra de 120 escolares de diversas instituciones educativas, a quienes se aplicaron satisfactoriamente encuestas, entrevistas y guía de evaluación física; también se analizó la publicidad emitida por los canales de televisión sobre alimentos y su contenido nutricional. El análisis inferencial aplicado fue Ji cuadrado (?2. Con esta investigación se ha demostrado que el estado de salud de los escolares está afectado, siendo muy agudo respecto a la caries dental, problema que se atribuye al consumo de ciertos alimentos. Se constata que existe relación positiva, con alta significancia estadística, entre la adherencia a los anuncios televisivos y el consumo de alimentos publicitados de alto contenido en sodio, calcio, azúcar y de calorías por los escolares estudiados; así mismo, se prueba la interdependencia entre la exposición a los anuncios televisivos y el consumo de alimentos con productos de alto contenido en grasa que merecen ser vigilados y controlados por los responsables.

  11. Benchmark critical experiments on low-enriched uranium oxide systems with H/U = 0. 77

    Energy Technology Data Exchange (ETDEWEB)

    Tuck, G; Oh, I

    1979-08-01

    Ten benchmark experiments were performed at the Critical Mass Laboratory at Rockwell International's Rocky Flats Plant, Golden, Colorado, for the US Nuclear Regulatory Commission. They provide accurate criticality data for low-enriched damp uranium oxide (U/sub 3/O/sub 8/) systems. The core studied consisted of 152 mm cubical aluminum cans containing an average of 15,129 g of low-enriched (4.46% /sup 235/U) uranium oxide compacted to a density of 4.68 g/cm/sup 3/ and with an H/U atomic ratio of 0.77. One hundred twenty five (125) of these cans were arranged in an approx. 770 mm cubical array. Since the oxide alone cannot be made critical in an array of this size, an enriched (approx. 93% /sup 235/U) metal or solution driver was used to achieve criticality. Measurements are reported for systems having the least practical reflection and for systems reflected by approx. 254-mm-thick concrete or plastic. Under the three reflection conditions, the mass of the uranium metal driver ranged from 29.87 kg to 33.54 kg for an oxide core of 1864.6 kg. For an oxide core of 1824.9 kg, the weight of the high concentration (351.2 kg U/m/sup 3/) solution driver varied from 14.07 kg to 16.14 kg, and the weight of the low concentration (86.4 kg U/m/sup 3/) solution driver from 12.4 kg to 14.0 kg.

  12. HuMiTar: A sequence-based method for prediction of human microRNA targets

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    Chen Ke

    2008-12-01

    Full Text Available Abstract Background MicroRNAs (miRs are small noncoding RNAs that bind to complementary/partially complementary sites in the 3' untranslated regions of target genes to regulate protein production of the target transcript and to induce mRNA degradation or mRNA cleavage. The ability to perform accurate, high-throughput identification of physiologically active miR targets would enable functional characterization of individual miRs. Current target prediction methods include traditional approaches that are based on specific base-pairing rules in the miR's seed region and implementation of cross-species conservation of the target site, and machine learning (ML methods that explore patterns that contrast true and false miR-mRNA duplexes. However, in the case of the traditional methods research shows that some seed region matches that are conserved are false positives and that some of the experimentally validated target sites are not conserved. Results We present HuMiTar, a computational method for identifying common targets of miRs, which is based on a scoring function that considers base-pairing for both seed and non-seed positions for human miR-mRNA duplexes. Our design shows that certain non-seed miR nucleotides, such as 14, 18, 13, 11, and 17, are characterized by a strong bias towards formation of Watson-Crick pairing. We contrasted HuMiTar with several representative competing methods on two sets of human miR targets and a set of ten glioblastoma oncogenes. Comparison with the two best performing traditional methods, PicTar and TargetScanS, and a representative ML method that considers the non-seed positions, NBmiRTar, shows that HuMiTar predictions include majority of the predictions of the other three methods. At the same time, the proposed method is also capable of finding more true positive targets as a trade-off for an increased number of predictions. Genome-wide predictions show that the proposed method is characterized by 1.99 signal

  13. miR-29b, miR-205 and miR-221 enhance chemosensitivity to gemcitabine in HuH28 human cholangiocarcinoma cells.

    Directory of Open Access Journals (Sweden)

    Kinya Okamoto

    Full Text Available BACKGROUND AND AIMS: Cholangiocarcinoma (CCA is highly resistant to chemotherapy, including gemcitabine (Gem treatment. MicroRNAs (miRNAs are endogenous, non-coding, short RNAs that can regulate multiple genes expression. Some miRNAs play important roles in the chemosensitivity of tumors. Here, we examined the relationship between miRNA expression and the sensitivity of CCA cells to Gem. METHODS: Microarray analysis was used to determine the miRNA expression profiles of two CCA cell lines, HuH28 and HuCCT1. To determine the effect of candidate miRNAs on Gem sensitivity, expression of each candidate miRNA was modified via either transfection of a miRNA mimic or transfection of an anti-oligonucleotide. Ontology-based programs were used to identify potential target genes of candidate miRNAs that were confirmed to affect the Gem sensitivity of CCA cells. RESULTS: HuCCT1 cells were more sensitive to Gem than were HuH28 cells, and 18 miRNAs were differentially expressed whose ratios over ± 2log2 between HuH28 and HuCCT1. Among these 18 miRNAs, ectopic overexpression of each of three downregulated miRNAs in HuH28 (miR-29b, miR-205, miR-221 restored Gem sensitivity to HuH28. Suppression of one upregulated miRNA in HuH28, miR-125a-5p, inhibited HuH28 cell proliferation independently to Gem treatment. Selective siRNA-mediated downregulation of either of two software-predicted targets, PIK3R1 (target of miR-29b and miR-221 or MMP-2 (target of miR-29b, also conferred Gem sensitivity to HuH28. CONCLUSIONS: miRNA expression profiling was used to identify key miRNAs that regulate Gem sensitivity in CCA cells, and software that predicts miRNA targets was used to identify promising target genes for anti-tumor therapies.

  14. MiR-291b-3p Induces Apoptosis in Liver Cell Line NCTC1469 by Reducing the Level of RNA-binding Protein HuR

    Directory of Open Access Journals (Sweden)

    Jun Guo

    2014-03-01

    Full Text Available Background: There is increasing evidence that miRNAs are involved in cellular apoptosis. However, the specific role of miR-291b-3p in apoptosis has not been elucidated. In the present study, we investigated the effect of miR-291b-3p on NCTC1469 cell growth and apoptosis. Methods: Cell viability and apoptosis were examined in NCTC1469 cells transfected with miR-291b-3p mimics, inhibitor miRNA or negative control. Using computational miRNA target prediction databases, HuR was predicted as a target of miR-291b-3p. Luciferase assay, immunofluorescence and western blot were used to further explore the effects of miR-291b-3p on HuR expression. In addition, the effect of HuR on cell apoptosis was evaluated using a HuR-specific siRNA. Results: TNF-α-induced hepatocyte apoptosis was accompanied by enhanced expression of miR-291b-3p, suggesting that miR-291b-3p might contribute to the apoptotic process. Follow-up experiments showed that upregulation of miR-291b-3p decreased cell viability and induced NCTC1469 cell apoptosis. Additionally, similar to the activity of miR-519, which is another member of the same miRNA family, miR-291b-3p suppressed HuR translation through binding to the HuR coding region (CR. We further showed that the downregulation of HuR expression by miR-291b-3p was accompanied by reduced Bcl-2 expression. Moreover, knockdown of HuR also impaired Bcl-2 expression and increased the ratio of Bax/Bcl-2. More significantly, downregulation of miR-291b-3p failed to increase Bcl2 expression in NCTC1469 cells that were co-transfected with siRNA-HuR. Finally, inhibition of miR-291b-3p led to reduced apoptosis, while knockdown of HuR by siRNA promoted apoptosis, even in NCTC1469 cells that were co-transfected with the miR-291b-3p inhibitor. Conclusion: The current data suggested that miR-291b-3p contributed to NCTC1469 cell apoptosis by regulating the expression of HuR, which in turn increased Bcl-2 stability.

  15. PKCε promotes HuD-mediated neprilysin mRNA stability and enhances neprilysin-induced Aβ degradation in brain neurons.

    Directory of Open Access Journals (Sweden)

    Chol Seung Lim

    Full Text Available Amyloid-beta (Aβ peptide accumulation in the brain is a pathological hallmark of all forms of Alzheimer's disease. An imbalance between Aβ production and clearance from the brain may contribute to accumulation of neurotoxic Aβ and subsequent synaptic loss, which is the strongest correlate of the extent of memory loss in AD. The activity of neprilysin (NEP, a potent Aβ-degrading enzyme, is decreased in the AD brain. Expression of HuD, an mRNA-binding protein important for synaptogenesis and neuronal plasticity, is also decreased in the AD brain. HuD is regulated by protein kinase Cε (PKCε, and we previously demonstrated that PKCε activation decreases Aβ levels. We hypothesized that PKCε acts through HuD to stabilize NEP mRNA, modulate its localization, and support NEP activity. Conversely, loss of PKCε-activated HuD in AD leads to decreased NEP activity and accumulation of Aβ. Here we show that HuD is associated with NEP mRNA in cultures of human SK-N-SH cells. Treatment with bryostatin, a PKCε-selective activator, enhanced NEP association with HuD and increased NEP mRNA stability. Activation of PKCε also increased NEP protein levels, increased NEP phosphorylation, and induced cell surface expression. In addition, specific PKCε activation directly stimulated NEP activity, leading to degradation of a monomeric form of Aβ peptide and decreased Aβ neuronal toxicity, as measured by cell viability. Bryostatin treatment also rescued Aβ-mediated inhibition of HuD-NEP mRNA binding, NEP protein expression, and NEP cell membrane translocation. These results suggest that PKCε activation reduces Aβ by up-regulating, via the mRNA-binding protein HuD, Aβ-degrading enzymes such as NEP. Thus, PKCε activation may have therapeutic efficacy for AD by reducing neurotoxic Aβ accumulation as well as having direct anti-apoptotic and synaptogenic effects.

  16. President Hu is in India but Who Am I in China?%胡主席在印度,我却在中国

    Institute of Scientific and Technical Information of China (English)

    Rajesh Kanoi (Jack)

    2007-01-01

    @@ Today, Sunday morning, I sit thinking and typing at my computer. My thoughts are in my homeland, India and my body is in China. The body is here and mind there for a good reason. Mr. Hu is president and I am resident and both are in each other's countries (what a difference the absence of one small letter makes!). Mr. Hu will return in a few days and I will stay on longer.

  17. HuGE Watch: tracking trends and patterns of published studies of genetic association and human genome epidemiology in near-real time.

    Science.gov (United States)

    Yu, Wei; Wulf, Anja; Yesupriya, Ajay; Clyne, Melinda; Khoury, Muin Joseph; Gwinn, Marta

    2008-09-01

    HuGE Watch is a web-based application for tracking the evolution of published studies on genetic association and human genome epidemiology in near-real time. The application allows users to display temporal trends and spatial distributions as line charts and google maps, providing a quick overview of progress in the field. http://www.hugenavigator.net/HuGENavigator/startPageWatch.do

  18. WBN-1 Cycle 10 TPBAR Tritium Release, Deduced From Analysis of RCS Data TTP-1-3046-00, Rev 0

    Energy Technology Data Exchange (ETDEWEB)

    Shaver, Mark W.; Niehus, Mark T.; Love, Edward F.

    2012-02-19

    This document contains the calculation of the TPBAR tritium release from the Mark 9.2 design TPBARs irradiated in WBN cycle 10. The calculation utilizes the generalized cycle analysis methodology given in TTP-1-3045 Rev. 0.

  19. DC Brushless Motor Control Design and Preliminary Testing for Independent 4-Wheel Drive Rev-11 Robotic Platform

    Directory of Open Access Journals (Sweden)

    Roni Permana Saputra

    2012-03-01

    Full Text Available This paper discusses the design of control system for brushless DC motor using microcontroller ATMega 16 that will be applied to an independent 4-wheel drive Mobile Robot LIPI version 2 (REV-11. The control system consists of two parts which are brushless DC motor control module and supervisory control module that coordinates the desired command to the motor control module. To control the REV-11 platform, supervisory control transmit the reference data of speed and direction of motor to control the speed and direction of each actuator on the platform REV-11. From the test results it is concluded that the designed control system work properly to coordinate and control the speed and direction of motion of the actuator motor REV-11 platform. 

  20. Horns Rev offshore wind farm. Environmental impact assessment of sea bottom and marine biology

    Energy Technology Data Exchange (ETDEWEB)

    Leonhard, S.B.

    2000-03-15

    An Environmental Impact Assessment (EIA) of a planned 150 MW offshore wind farm at Horns Rev has been carried out for the marine biology and sea bottom in the area, and includes vegetation and benthic fauna. The study forms part of a total EIA of the planned offshore wind farm. This EIA study has been drawn up in accordance with the guidelines laid down by the Ministry of Environment and Energy in the publication, 'Guidelines for preparation of EIAstudies for offshore wind farms. Horns Rev is situated off Blaevands Huk, which is Denmark's most westerly point. It is a shallow reef with water depths between 2 and 9 metres and is primarily composed of sand, gravel and pebbles. The area designated for the wind farm lies directly south of Horns Rev and is dominated by sand with a median particle size of 0.3 mm. Along the edges, towards areas of greater depth, the particle size increases. There are areas of fine sand in the deepest area, and in isolated pockets within the proposed wind farm site. The sediment is characterised by a very low (<1%) organic matter content. On the basis of the expected impact from the establishment of the wind farm, it is not deemed necessary to carry out special programmes during the construction phase for monitoring of the environmental-biological conditions. A monitoring and control programme is recommended during the production phase in order to follow the copper concentration in bivalves, or alternatively to initiate recovery or elimination of the copper-laden waste. A control programme is recommended during the production phase in order to follow the establishment and succession of the fouling community on the wind turbine foundations and scour-protecting revetments. (BA)

  1. Pilot power based rate control in CDMA 2000 1x EV-DO Rev-A

    Institute of Scientific and Technical Information of China (English)

    LIU Xiao-feng; GU Jian; YANG Hong-wen

    2008-01-01

    This article proposes a new algorithm to improvethe rate control efficiency of enhanced reverse link mediumaccess control (RLMAC) in the code division multiple access(CDMA) lx EV-DO release A(Rev. A) system. The newalgorithm brings reverse access terminal (AT) pilot power tothe RLMAC rate control procedure and makes it easier for alow pilot power user to increase its data rate when the systemis slightly loaded and harder to decrease its date rate when thesystem is heavily loaded. Numerical results of system levelsimulations show that the new algorithm can bring highersystem throughput, lower AT transmission power, and lowersystem load.

  2. De novo mutations in Plxnd1 and Rev3l cause mobius syndrome

    OpenAIRE

    Kayserili Karabey, Hülya; Tomas-Roca, Laura; Tsaalbi-Shtylik, Anastasia; Jansen, Jacob G.; Singh, Manvendra K.; Epstein, Jonathan A.; Altunoglu, Umut; Verzijl, Harriette; Soria, Laura; van Beusekom, Ellen; Roscioli, Tony; Iqbal, Zafar; Gilissen, Christian; Hoischen, Alexander; de Brouwer,Arjan P. M.; Erasmus, Corrie; Schubert, Dirk; Brunner, Han; Aytes, Antonio Perez; Marin, Faustino; Aroca, Pilar; Carta, Arturo; de Wind, Niels; Padberg, George W.; van Bokhoven, Hans

    2015-01-01

    ARTICLE Received 15 Nov 2014 | Accepted 17 Apr 2015 | Published 12 Jun 2015 De novo mutations in PLXND1 and REV3L cause Mo¨bius syndrome Laura Tomas-Roca1,2, Anastasia Tsaalbi-Shtylik3, Jacob G. Jansen3, Manvendra K. Singh4,5, Jonathan A. Epstein4, Umut Altunoglu6, Harriette Verzijl7, Laura Soria1, Ellen van Beusekom1, Tony Roscioli1,8, Zafar Iqbal1, Christian Gilissen1, Alexander Hoischen9, Arjan P.M. de Brouwer1, Corrie Erasmus7, Dirk Schubert10, Han Brunner1,11, Antoni...

  3. Simulaton of the Avedøreværket Unit 1 Cogeneration Plant with DNA

    DEFF Research Database (Denmark)

    Elmegaard, Brian; Houbak, Niels

    2003-01-01

    The simulator contest proposed for the ECOS 2003 conference has been solved using the DNA energy system simulator. The contest concerns the steam process of the Avedøreværket Unit 1 (AVV1) power plant. The plant is a 250 MWCHP plant with a maximum district heat production of 330 MJ/s. The plant has...... a brief description of the DNA simulator, as well as a short notice on some of the component models used. In particular, there is a small investigation of the difference obtained in the solution depending on the model used for the turbine (isentropic efficiency, polytropic efficiency, or straight...

  4. Bird numbers and distributions in the Horns Rev offshore wind farm area. Annual status report 2004

    Energy Technology Data Exchange (ETDEWEB)

    Krag Petersen, I.

    2005-07-01

    This report presents data from four aerial surveys of birds in the Horns Rev wind farm area in 2004. Three surveys from the winter and spring of 2004 are thoroughly reported here. The fourth survey of 9 September 2004 is reported in general terms, but not included in presentations of distribution and effect analyses of the wind farm. Data from this survey will be thoroughly dealt with in a future report. Including the four surveys of 2004, a total of 29 surveys have been performed in that area since August 1999. (au)

  5. RNA-binding protein HuR sequesters microRNA-21 to prevent translation repression of proinflammatory tumor suppressor gene programmed cell death 4.

    Science.gov (United States)

    Poria, D K; Guha, A; Nandi, I; Ray, P S

    2016-03-31

    Translation control of proinflammatory genes has a crucial role in regulating the inflammatory response and preventing chronic inflammation, including a transition to cancer. The proinflammatory tumor suppressor protein programmed cell death 4 (PDCD4) is important for maintaining the balance between inflammation and tumorigenesis. PDCD4 messenger RNA translation is inhibited by the oncogenic microRNA, miR-21. AU-rich element-binding protein HuR was found to interact with the PDCD4 3'-untranslated region (UTR) and prevent miR-21-mediated repression of PDCD4 translation. Cells stably expressing miR-21 showed higher proliferation and reduced apoptosis, which was reversed by HuR expression. Inflammatory stimulus caused nuclear-cytoplasmic relocalization of HuR, reversing the translation repression of PDCD4. Unprecedentedly, HuR was also found to bind to miR-21 directly, preventing its interaction with the PDCD4 3'-UTR, thereby preventing the translation repression of PDCD4. This suggests that HuR might act as a 'miRNA sponge' to regulate miRNA-mediated translation regulation under conditions of stress-induced nuclear-cytoplasmic translocation of HuR, which would allow fine-tuned gene expression in complex regulatory environments.

  6. Changes in Cellular mRNA Stability, Splicing, and Polyadenylation through HuR Protein Sequestration by a Cytoplasmic RNA Virus

    Directory of Open Access Journals (Sweden)

    Michael D. Barnhart

    2013-11-01

    Full Text Available The impact of RNA viruses on the posttranscriptional regulation of cellular gene expression is unclear. Sindbis virus causes a dramatic relocalization of the cellular HuR protein from the nucleus to the cytoplasm in infected cells. This is to the result of the expression of large amounts of viral RNAs that contain high-affinity HuR binding sites in their 3′ UTRs effectively serving as a sponge for the HuR protein. Sequestration of HuR by Sindbis virus is associated with destabilization of cellular mRNAs that normally bind HuR and rely on it to regulate their expression. Furthermore, significant changes can be observed in nuclear alternative polyadenylation and splicing events on cellular pre-mRNAs as a result of sequestration of HuR protein by the 3′ UTR of transcripts of this cytoplasmic RNA virus. These studies suggest a molecular mechanism of virus-host interaction that probably has a significant impact on virus replication, cytopathology, and pathogenesis.

  7. UVB-dependent changes in the expression of fast-responding early genes is modulated by huCOP1 in keratinocytes.

    Science.gov (United States)

    Fazekas, B; Polyánka, H; Bebes, A; Tax, G; Szabó, K; Farkas, K; Kinyó, A; Nagy, F; Kemény, L; Széll, M; Ádám, É

    2014-11-01

    Ultraviolet (UV) B is the most prominent physical carcinogen in the environment leading to the development of various skin cancers. We have previously demonstrated that the human ortholog of the Arabidopsis thaliana constitutive photomorphogenesis 1 (COP1) protein, huCOP1, is expressed in keratinocytes in a UVB-regulated manner and is a negative regulator of p53 as a posttranslational modifier. However, it was not known whether huCOP1 plays a role in mediating the UVB-induced early transcriptional responses of human keratinocytes. In this study, we report that stable siRNA-mediated silencing of huCOP1 affects the UVB response of several genes within 2 h of irradiation, indicating that altered huCOP1 expression sensitizes the cells toward UVB. Pathway analysis identified a molecular network in which 13 of the 30 examined UVB-regulated genes were organized around three central proteins. Since the expression of the investigated genes was upregulated by UVB in the siCOP1 cell line, we hypothesize that huCOP1 is a repressor of the identified pathway. Several members of the network have been implicated previously in the pathogenesis of non-melanoma skin cancers; therefore, clarifying the role of huCOP1 in these skin diseases may have clinical relevance in the future.

  8. CTL Responses to Regulatory Proteins Tat and Rev in HIV-1 B'/C Virus-Infected Individuals

    Institute of Scientific and Technical Information of China (English)

    MING-MING JIA; KUN-XUE HONG; JIAN-PING CHEN; HONG-WEI LIU; SHA LIU; XIAO-QING ZHANG; HONG-JING ZHAO; YI-MING SHAO

    2008-01-01

    To characterize HIV-1 specific CTL responses to regulatory proteins Tat and Rev in HIV-B'/C vires-infected ART-naive individuals. Methods HIV-1-specific CTL responses were analyzed by IFN-γ ELISPOT assay using overlapping peptides spanning the consensus sequences of HIV-1 clade C Tat and Rev proteins. Statistical analysis and graphical presentation were performed using SIGMAPLOT 10.0 and SIGMASTAT 3.5. For samples with a positive response, the magnitude of CTL responses was compared between HIV-1 C proteins by Wilcoxon rank sum test, and the significance threshold was P<0.05. Results Tat and Rev were frequently recognized, with 23% and 52% of the tested individuals having detectable responses to these proteins, respectively. Several immunodominant regions were detected in Rev. No significant correlation was observed between the magnitude and breadth of CTL responses to regulatory proteins and the control of virus replication in this study. Conclusion Tat and Rev can serve as targets for HIV-1-specific CTL, and several immunodominant regions are detectable in Rev. Further characterization of epitopes and their role in virus control may shed light on pathogenesis of HIV-1 natural infection and also be useful for the design and testing of candidate vaccines.

  9. HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment.

    Science.gov (United States)

    Taniguchi, Ichiro; Mabuchi, Naoto; Ohno, Mutsuhito

    2014-06-01

    Nuclear RNA export pathways in eukaryotes are often linked to the fate of a given RNA. Therefore, the choice of export pathway should be well-controlled to avoid an unfavorable effect on gene expression. Although some RNAs could be exported by more than one pathway, little is known about how the choice is regulated. This issue is highlighted when the human immunodeficiency virus type 1 (HIV-1) Rev protein induces the export of singly spliced and unspliced HIV-1 transcripts. How these RNAs are exported is not well understood because such transcripts should have the possibility of utilizing CRM1-dependent export via Rev or cellular TAP/NXF1-dependent export via the transcription/export (TREX) complex, or both. Here we found that Rev suppressed TAP/NXF1-dependent export of model RNA substrates that recapitulated viral transcripts. In this effect, Rev interacted with the cap-binding complex and inhibited the recruitment of the TREX complex. Thus, Rev controls the identity of the factor occupying the cap-proximal region that determines the RNA export pathway. This ribonucleoprotein remodeling activity of Rev may favor viral gene expression.

  10. Squeezed Phonons: Modulating Quantum Fluctuations of Atomic Displacements.

    Science.gov (United States)

    Hu, Xuedong; Nori, Franco

    1997-03-01

    We have studied phonon squeezed states and also put forward several proposals for their generation(On phonon parametric process, X. Hu and F. Nori, Phys. Rev. Lett. 76), 2294 (1996); on polariton mechanism, X. Hu and F. Nori, Phys. Rev. B 53, 2419 (1996); on second-order Raman scattering, X. Hu and F. Nori, preprint.. Here, we compare the relative merits and limitations of these approaches, including several factors that will limit the amount of phonon squeezing. In particular, we investigate the effect of the initial thermal states on the phonon modes. Using a model for the phonon density matrix, we also study the mixing of the phonon squeezed states with thermal states, which describes the decay of the phonon coherence. Finally, we calculate the maximum possible squeezing from a phonon parametric process limited by phonon decay.

  11. Effect of HU308 on microglia induced by LPS in secretion of NO and IL-6%HU308对脂多糖诱导小胶质细胞分泌NO及IL-6的影响

    Institute of Scientific and Technical Information of China (English)

    赵建云; 邓展进; 刘瑞珍

    2014-01-01

    Objective To study the effect in secretion of NO and IL-6 of cannabinoid CB2 receptor agonist (HU308) on microglia induced by lipopolysaccharide (LPS). Methods vitro culture BV-2 microglia were divided into control group, LPS stimulation group and intervention group (LPS + HU308). The morphological changes in microglia were observed by microscope, the proliferation in microglia was detected by CCK-8, the NO contents were detected by Griess reaction, the IL-6 contents were detected by ELISA. Results A large number of microglia in LPS stimulation group showed auxetic body, stubby or disappeared pseudopodium and necrotic cells, cell proliferation was poor, the expression level of NO and IL-6 increased significantly. After the intervention of CB2 receptor agonist (HU308), most of microglia showed slightly large body, obvious pseudopodia and light damage, cell proliferation was better, the expression of inflammatory factor decreased significantly. Conclusion Activating CB2 receptors expressing in microglia can alleviate the excessive activation of microglia caused by LPS, inhibit the secretion of N0 and IL-6, so as to achieve the protective effect of central nervous system inflammatory injury.%目的:研究大麻素CB2受体激动剂HU308对脂多糖(LPS)诱导小胶质细胞激活后NO及IL-6分泌的影响。方法体外培养小鼠小胶质细胞株(BV-2细胞),分为对照组、LPS刺激组及干预组(LPS+HU308)。通过显微镜观察各组小胶质细胞的形态学变化,CCK-8法检测各组小胶质细胞的增殖情况,Griess法检测各组NO含量,ELISA法检测各组IL-6水平。结果LPS刺激组的小胶质细胞中出现大量胞体增大,伪足粗短或消失的细胞和一些坏死细胞,细胞增殖较差,NO及IL-6表达水平显著增高。经大麻素CB2受体激动剂HU308干预后,大部分细胞胞体稍大,伪足尚明显,细胞破坏程度轻,增殖较好,炎性因子表达均明显下降。结论激动小

  12. A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity

    Institute of Scientific and Technical Information of China (English)

    SUN AiJun; LAWRENCE Petherbridge; ZHAO YuGuang; LI YanPeng; NAIR Venugopal K; CUI ZhiZhong

    2009-01-01

    The complete genome of Marek's disease virus (MDV) strain GX0101,which was integrated with the LTR sequences of REV,was cloned in Escherichia coli as a bacterial artificial chromosome (BAC).BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination.The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B.Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF).This BAC viral clone was named bac-GX0101.When the reconstituted virus was inoculated into 1-day-old birds,visceral tumors could be detected as early as 62 d post infection.There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus.The BAC derived virus maintained its oncogenicity and immunosuppressive effects.In conclusion,the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued.With the powerful BAC manipulation system,the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV.

  13. High amplitude phase resetting in rev-erbalpha/per1 double mutant mice.

    Directory of Open Access Journals (Sweden)

    Corinne Jud

    Full Text Available Over time, organisms developed various strategies to adapt to their environment. Circadian clocks are thought to have evolved to adjust to the predictable rhythms of the light-dark cycle caused by the rotation of the Earth around its own axis. The rhythms these clocks generate persist even in the absence of environmental cues with a period of about 24 hours. To tick in time, they continuously synchronize themselves to the prevailing photoperiod by appropriate phase shifts. In this study, we disrupted two molecular components of the mammalian circadian oscillator, Rev-Erbalpha and Period1 (Per1. We found that mice lacking these genes displayed robust circadian rhythms with significantly shorter periods under constant darkness conditions. Strikingly, they showed high amplitude resetting in response to a brief light pulse at the end of their subjective night phase, which is rare in mammals. Surprisingly, Cry1, a clock component not inducible by light in mammals, became slightly inducible in these mice. Taken together, Rev-Erbalpha and Per1 may be part of a mechanism preventing drastic phase shifts in mammals.

  14. Purification and characterization of a novel chlorpyrifos hydrolase from Cladosporium cladosporioides Hu-01.

    Science.gov (United States)

    Gao, Yan; Chen, Shaohua; Hu, Meiying; Hu, Qiongbo; Luo, Jianjun; Li, Yanan

    2012-01-01

    Chlorpyrifos is of great environmental concern due to its widespread use in the past several decades and its potential toxic effects on human health. Thus, the degradation study of chlorpyrifos has become increasing important in recent years. A fungus capable of using chlorpyrifos as the sole carbon source was isolated from organophosphate-contaminated soil and characterized as Cladosporium cladosporioides Hu-01 (collection number: CCTCC M 20711). A novel chlorpyrifos hydrolase from cell extract was purified 35.6-fold to apparent homogeneity with 38.5% overall recovery by ammoniumsulfate precipitation, gel filtration chromatography and anion-exchange chromatography. It is a monomeric structure with a molecular mass of 38.3 kDa. The pI value was estimated to be 5.2. The optimal pH and temperature of the purified enzyme were 6.5 and 40°C, respectively. No cofactors were required for the chlorpyrifos-hydrolysis activity. The enzyme was strongly inhibited by Hg²⁺, Fe³⁺, DTT, β-mercaptoethanol and SDS, whereas slight inhibitory effects (5-10% inhibition) were observed in the presence of Mn²⁺, Zn²⁺, Cu²⁺, Mg²⁺, and EDTA. The purified enzyme hydrolyzed various organophosphorus insecticides with P-O and P-S bond. Chlorpyrifos was the preferred substrate. The Km and Vmax values of the enzyme for chlorpyrifos were 6.7974 μM and 2.6473 μmol·min⁻¹, respectively. Both NH2-terminal sequencing and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometer (MALDI-TOF-MS) identified an amino acid sequence MEPDGELSALTQGANS, which shared no similarity with any reported organophosphate-hydrolyzing enzymes. These results suggested that the purified enzyme was a novel hydrolase and might conceivably be developed to fulfill the practical requirements to enable its use in situ for detoxification of chlorpyrifos. Finally, this is the first described chlorpyrifos hydrolase from fungus.

  15. Response to "Comment on ‘Electric Multipole Moments for Some First-Row Diatomic Hydride Molecules' [Commun. Theor. Phys. 38 (2002) 256]"

    Institute of Scientific and Technical Information of China (English)

    Metin Orbay; Telhat Ozdogan

    2003-01-01

    The comment of Guseinov is irrelevant and also unjust. In contrast to his comment, we show that theobtained electric multipole moment values for some first-row diatomic molecules are original and better than his values(I.I. Guseinov, E. Akin, and A.M. Rzaeva, J. Mol. Struct. (Theochem) 453 (1998) 163) with respect to Hartree-Fockvalues. Moreover, it must be noted that all the formulas are cited in our paper (M. Orbay and T. Ozdogan, Commun.Theor. Phys. (Beijing, China) 35 (2001) 585) and corrigendum (M. Orbay and T. Ozdogan, Commun. Theor. Phys.(Beijing, China) 37 (2002) 768).

  16. A Bite of Hu Flavour in Diets of Tang Dynasty%唐代舌尖上的胡味

    Institute of Scientific and Technical Information of China (English)

    刘锡涛

    2015-01-01

    Tang Dynasty was an innovative, open and tolerant society with booming economy and constant re-newals, which was characterized by strong Hu flavor in diet. There were plenty kinds of Hu diets on staples, vegetables, fruits, wines and flavourings. While pastries were the staple diet, there were such vegetables as spinach, cucumber, eggplant, preserved pickle, lettuce and such fruits as golden peach, grape, date and fla-vourings as sugar, parsley, pepper and such wine as grape wine, long-gao wine. The popularity of Hu diet in Tang Dynasty accelerates the multi-ethnic mélange of dietetic cultures.%唐代是个改革、开放与包容的社会,经济发展,吐旧纳新,人们在饮食上多体现出胡味现象,胡食品种繁多,集中体现在主食、蔬菜、水果、酒及调味品上。主食多吃面饼,蔬菜主要有菠菜、黄瓜、茄子、榨菜、莴苣等,水果有金桃、葡萄、波斯枣等,调味品主要有蔗糖、香菜、胡椒等,酒有葡萄酒、龙膏酒等水果酒。唐代胡食的盛行,加快了我国多民族饮食文化的融合发展。

  17. The structural basis of gas-responsive transcription by the human nuclear hormone receptor REV-ERBbeta.

    Directory of Open Access Journals (Sweden)

    Keith I Pardee

    2009-02-01

    Full Text Available Heme is a ligand for the human nuclear receptors (NR REV-ERBalpha and REV-ERBbeta, which are transcriptional repressors that play important roles in circadian rhythm, lipid and glucose metabolism, and diseases such as diabetes, atherosclerosis, inflammation, and cancer. Here we show that transcription repression mediated by heme-bound REV-ERBs is reversed by the addition of nitric oxide (NO, and that the heme and NO effects are mediated by the C-terminal ligand-binding domain (LBD. A 1.9 A crystal structure of the REV-ERBbeta LBD, in complex with the oxidized Fe(III form of heme, shows that heme binds in a prototypical NR ligand-binding pocket, where the heme iron is coordinately bound by histidine 568 and cysteine 384. Under reducing conditions, spectroscopic studies of the heme-REV-ERBbeta complex reveal that the Fe(II form of the LBD transitions between penta-coordinated and hexa-coordinated structural states, neither of which possess the Cys384 bond observed in the oxidized state. In addition, the Fe(II LBD is also able to bind either NO or CO, revealing a total of at least six structural states of the protein. The binding of known co-repressors is shown to be highly dependent upon these various liganded states. REV-ERBs are thus highly dynamic receptors that are responsive not only to heme, but also to redox and gas. Taken together, these findings suggest new mechanisms for the systemic coordination of molecular clocks and metabolism. They also raise the possibility for gas-based therapies for the many disorders associated with REV-ERB biological functions.

  18. The structural basis of gas-responsive transcription by the human nuclear hormone receptor REV-ERBbeta.

    Science.gov (United States)

    Pardee, Keith I; Xu, Xiaohui; Reinking, Jeff; Schuetz, Anja; Dong, Aiping; Liu, Suya; Zhang, Rongguang; Tiefenbach, Jens; Lajoie, Gilles; Plotnikov, Alexander N; Botchkarev, Alexey; Krause, Henry M; Edwards, Aled

    2009-02-24

    Heme is a ligand for the human nuclear receptors (NR) REV-ERBalpha and REV-ERBbeta, which are transcriptional repressors that play important roles in circadian rhythm, lipid and glucose metabolism, and diseases such as diabetes, atherosclerosis, inflammation, and cancer. Here we show that transcription repression mediated by heme-bound REV-ERBs is reversed by the addition of nitric oxide (NO), and that the heme and NO effects are mediated by the C-terminal ligand-binding domain (LBD). A 1.9 A crystal structure of the REV-ERBbeta LBD, in complex with the oxidized Fe(III) form of heme, shows that heme binds in a prototypical NR ligand-binding pocket, where the heme iron is coordinately bound by histidine 568 and cysteine 384. Under reducing conditions, spectroscopic studies of the heme-REV-ERBbeta complex reveal that the Fe(II) form of the LBD transitions between penta-coordinated and hexa-coordinated structural states, neither of which possess the Cys384 bond observed in the oxidized state. In addition, the Fe(II) LBD is also able to bind either NO or CO, revealing a total of at least six structural states of the protein. The binding of known co-repressors is shown to be highly dependent upon these various liganded states. REV-ERBs are thus highly dynamic receptors that are responsive not only to heme, but also to redox and gas. Taken together, these findings suggest new mechanisms for the systemic coordination of molecular clocks and metabolism. They also raise the possibility for gas-based therapies for the many disorders associated with REV-ERB biological functions.

  19. Les structures génotypiques multilocus des populations d'huîtres plates en France

    Directory of Open Access Journals (Sweden)

    Françoise BLANC

    1987-09-01

    Full Text Available La structure génétique des populations de l'huître plate Ostrea edulis est décrite sur la base des génotypes multilocus. Associée au traitement des données de type multivarié, cette nouvelle procédure permet d'identifier les sous-ensembles génétiques intrapopulationnels qui peuvent être repérés dans un échantillon composite résultant de transferts comme dans la lagune de Thau.

  20. pp32 (ANP32A expression inhibits pancreatic cancer cell growth and induces gemcitabine resistance by disrupting HuR binding to mRNAs.

    Directory of Open Access Journals (Sweden)

    Timothy K Williams

    Full Text Available The expression of protein phosphatase 32 (PP32, ANP32A is low in poorly differentiated pancreatic cancers and is linked to the levels of HuR (ELAV1, a predictive marker for gemcitabine response. In pancreatic cancer cells, exogenous overexpression of pp32 inhibited cell growth, supporting its long-recognized role as a tumor suppressor in pancreatic cancer. In chemotherapeutic sensitivity screening assays, cells overexpressing pp32 were selectively resistant to the nucleoside analogs gemcitabine and cytarabine (ARA-C, but were sensitized to 5-fluorouracil; conversely, silencing pp32 in pancreatic cancer cells enhanced gemcitabine sensitivity. The cytoplasmic levels of pp32 increased after cancer cells are treated with certain stressors, including gemcitabine. pp32 overexpression reduced the association of HuR with the mRNA encoding the gemcitabine-metabolizing enzyme deoxycytidine kinase (dCK, causing a significant reduction in dCK protein levels. Similarly, ectopic pp32 expression caused a reduction in HuR binding of mRNAs encoding tumor-promoting proteins (e.g., VEGF and HuR, while silencing pp32 dramatically enhanced the binding of these mRNA targets. Low pp32 nuclear expression correlated with high-grade tumors and the presence of lymph node metastasis, as compared to patients' tumors with high nuclear pp32 expression. Although pp32 expression levels did not enhance the predictive power of cytoplasmic HuR status, nuclear pp32 levels and cytoplasmic HuR levels associated significantly in patient samples. Thus, we provide novel evidence that the tumor suppressor function of pp32 can be attributed to its ability to disrupt HuR binding to target mRNAs encoding key proteins for cancer cell survival and drug efficacy.

  1. Saturated fatty acids induce post-transcriptional regulation of HAMP mRNA via AU-rich element-binding protein, human antigen R (HuR).

    Science.gov (United States)

    Lu, Sizhao; Mott, Justin L; Harrison-Findik, Duygu Dee

    2015-10-02

    Iron is implicated in fatty liver disease pathogenesis. The human hepcidin gene, HAMP, is the master switch of iron metabolism. The aim of this study is to investigate the regulation of HAMP expression by fatty acids in HepG2 cells. For these studies, both saturated fatty acids (palmitic acid (PA) and stearic acid (SA)) and unsaturated fatty acid (oleic acid (OA)) were used. PA and, to a lesser extent, SA, but not OA, up-regulated HAMP mRNA levels, as determined by real-time PCR. To understand whether PA regulates HAMP mRNA at the transcriptional or post-transcriptional level, the transcription inhibitor actinomycin D was employed. PA-mediated induction of HAMP mRNA expression was not blocked by actinomycin D. Furthermore, PA activated HAMP 3'-UTR, but not promoter, activity, as shown by reporter assays. HAMP 3'-UTR harbors a single AU-rich element (ARE). Mutation of this ARE abolished the effect of PA, suggesting the involvement of ARE-binding proteins. The ARE-binding protein human antigen R (HuR) stabilizes mRNA through direct interaction with AREs on 3'-UTR. HuR is regulated by phosphorylation-mediated nucleo-cytoplasmic shuttling. PA activated this process. The binding of HuR to HAMP mRNA was also induced by PA in HepG2 cells. Silencing of HuR by siRNA abolished PA-mediated up-regulation of HAMP mRNA levels. PKC is known to phosphorylate HuR. Staurosporine, a broad-spectrum PKC inhibitor, inhibited both PA-mediated translocation of HuR and induction of HAMP expression. Similarly, rottlerin, a novel class PKC inhibitor, abrogated PA-mediated up-regulation of HAMP expression. In conclusion, lipids mediate post-transcriptional regulation of HAMP throughPKC- and HuR-dependent mechanisms.

  2. HuR and podoplanin expression is associated with a high risk of malignant transformation in patients with oral preneoplastic lesions

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Higashino, Fumihiro; Hida, Kyoko; Totsuka, Yasunori; Shindoh, Masanobu

    2016-01-01

    The risk of malignant transformation in oral preneoplastic lesions (OPLs) is challenging to assess. The objective of the present study was to determine the expression of ELAV like RNA binding protein 1 (HuR) and podoplanin in OPLs, and to evaluate the use of each protein as biomarkers for the risk assessment of malignant transformations. Immunohistochemistry for HuR and podoplanin was performed on the tissues of 51 patients with OPL, including cases of low grade dysplasia (LGD) and high grade dysplasia (HGD). The association between the protein expression patterns and clinicopathological parameters, including oral cancer free survival (OCFS) time, was analyzed during the follow-up period. HuR and podoplanin expression was observed in 28 (55%) and 36 (71%) of 51 patients, respectively. Kaplan-Meier analysis showed that the expression of HuR and podoplanin was associated with the risk of progression to oral cancer (P<0.05). Multivariate analysis revealed that HuR and podoplanin expression was associated with a 2.93-fold (95% confidence interval (CI), 0.98–10.34; P=0.055) and 2.06-fold (95% CI, 0.55–8.01; P=0.283) increase in risk of malignant transformation, respectively. The risk of OPL malignant transformation was considerably increased with the coexpression of HuR and podoplanin compared with the histological grading (95% CI, 1.64–23.59; P=0.005). The results of the present study demonstrated that the expression of HuR and podoplanin associates with malignant transformation and suggests that the proteins may be used as biomarkers to identify OPL patients with an increased risk of cancer development. PMID:27899983

  3. GC–MS-Based Metabonomic Profiling Displayed Differing Effects of Borna Disease Virus Natural Strain Hu-H1 and Laboratory Strain V Infection in Rat Cortical Neurons

    Directory of Open Access Journals (Sweden)

    Siwen Liu

    2015-08-01

    Full Text Available Borna disease virus (BDV persists in the central nervous systems of a wide variety of vertebrates and causes behavioral disorders. Previous studies have revealed that metabolic perturbations are associated with BDV infection. However, the pathophysiological effects of different viral strains remain largely unknown. Rat cortical neurons infected with human strain BDV Hu-H1, laboratory BDV Strain V, and non-infected control (CON cells were cultured in vitro. At day 12 post-infection, a gas chromatography coupled with mass spectrometry (GC–MS metabonomic approach was used to differentiate the metabonomic profiles of 35 independent intracellular samples from Hu-H1-infected cells (n = 12, Strain V-infected cells (n = 12, and CON cells (n = 11. Partial least squares discriminant analysis (PLS-DA was performed to demonstrate discrimination between the three groups. Further statistical testing determined which individual metabolites displayed significant differences between groups. PLS-DA demonstrated that the whole metabolic pattern enabled statistical discrimination between groups. We identified 31 differential metabolites in the Hu-H1 and CON groups (21 decreased and 10 increased in Hu-H1 relative to CON, 35 differential metabolites in the Strain V and CON groups (30 decreased and 5 increased in Strain V relative to CON, and 21 differential metabolites in the Hu-H1 and Strain V groups (8 decreased and 13 increased in Hu-H1 relative to Strain V. Comparative metabonomic profiling revealed divergent perturbations in key energy and amino acid metabolites between natural strain Hu-H1 and laboratory Strain V of BDV. The two BDV strains differentially alter metabolic pathways of rat cortical neurons in vitro. Their systematic classification provides a valuable template for improved BDV strain definition in future studies.

  4. Increase of the RNA-binding protein HuD and posttranscriptional up-regulation of the GAP-43 gene during spatial memory

    Science.gov (United States)

    Pascale, Alessia; Gusev, Pavel A.; Amadio, Marialaura; Dottorini, Tania; Govoni, Stefano; Alkon, Daniel L.; Quattrone, Alessandro

    2004-01-01

    Neuronal ELAV-like proteins (HuB, HuC, and HuD) are highly conserved RNA-binding proteins able to selectively associate with the 3′ UTR of a subset of target mRNAs and increase their cytoplasmic stability and rate of translation. We previously demonstrated the involvement of these proteins in learning, reporting that they undergo a sustained up-regulation in the hippocampus of mice trained in a spatial discrimination task. Here, we extend this finding, showing that a similar up-regulation occurs in the hippocampus of rats trained in another spatial learning paradigm, the Morris water maze. HuD, a strictly neuron-specific ELAV-like protein, is shown to increase after learning, with a preferential binding to the cytoskeletal fraction. HuD up-regulation is associated with an enhancement of GAP-43 mRNA and protein levels, with an apparently increased HuD colocalization with the GAP-43 mRNA and an increased association of neuronal ELAV-like proteins with the GAP-43 mRNA. These learning-dependent biochemical events appear to be spatiotemporally controlled, because they do not occur in another brain region involved in learning, the retrosplenial cortex, and at the level of protein expression they show extinction 1 month after training despite memory retention. By contrast, HuD mRNA levels still remain increased after 1 month in the CA1 region. This persistence may have implications for long-term memory recall. PMID:14745023

  5. HuR and podoplanin expression is associated with a high risk of malignant transformation in patients with oral preneoplastic lesions.

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Higashino, Fumihiro; Hida, Kyoko; Totsuka, Yasunori; Shindoh, Masanobu

    2016-11-01

    The risk of malignant transformation in oral preneoplastic lesions (OPLs) is challenging to assess. The objective of the present study was to determine the expression of ELAV like RNA binding protein 1 (HuR) and podoplanin in OPLs, and to evaluate the use of each protein as biomarkers for the risk assessment of malignant transformations. Immunohistochemistry for HuR and podoplanin was performed on the tissues of 51 patients with OPL, including cases of low grade dysplasia (LGD) and high grade dysplasia (HGD). The association between the protein expression patterns and clinicopathological parameters, including oral cancer free survival (OCFS) time, was analyzed during the follow-up period. HuR and podoplanin expression was observed in 28 (55%) and 36 (71%) of 51 patients, respectively. Kaplan-Meier analysis showed that the expression of HuR and podoplanin was associated with the risk of progression to oral cancer (P<0.05). Multivariate analysis revealed that HuR and podoplanin expression was associated with a 2.93-fold (95% confidence interval (CI), 0.98-10.34; P=0.055) and 2.06-fold (95% CI, 0.55-8.01; P=0.283) increase in risk of malignant transformation, respectively. The risk of OPL malignant transformation was considerably increased with the coexpression of HuR and podoplanin compared with the histological grading (95% CI, 1.64-23.59; P=0.005). The results of the present study demonstrated that the expression of HuR and podoplanin associates with malignant transformation and suggests that the proteins may be used as biomarkers to identify OPL patients with an increased risk of cancer development.

  6. Construction of Eukaryotic Expression Vector Containing HuCTLA4Ig and its Expression in Mammalian Cells%HuCTLA4Ig真核表达载体的构建及其表达

    Institute of Scientific and Technical Information of China (English)

    祁小平; 钱军华; 谢晋; 马宝骊

    2000-01-01

    用RT-PCR方法从人外周血单个核细胞总RNA中分别扩增出CTLA4的胞膜外区基因和Igγ1恒定区基因,将它们拼接后插入质粒pcDNA3.将重组的HuCTLA4Ig/pcDNA质粒转染细胞,经筛选得到了高效、稳定表达HuCTLA4Ig的克隆.培养上清经ProteinA亲和层析柱纯化后,所得蛋白在非还原条件下SDS-PAGE呈分子量为11kD的一条带;FACS检测显示该纯化蛋白与B7分子有良好的结合能力;在体外能抑制MLR的增殖反应.

  7. Comment on "A new exactly solvable quantum model in $N$ dimensions" [Phys. Lett. A 375(2011)1431, arXiv:1007.1335

    CERN Document Server

    Ley, B L Moreno

    2011-01-01

    We pinpoint that the work about "a new exactly solvable quantum model in $N$ dimensions" by Ballesteros et al. [Phys. Lett. A {\\bf 375} (2011) 1431, arXiv:1007.1335] is not a new exactly solvable quantum model since the flaw of the position-dependent mass Hamiltonian proposed by them makes it less valuable in physics.

  8. Comment on ``Barut-Girardello and Klauder-Perelomov coherent states for the Kravchuk functions'' [J. Math. Phys. 48, 112106 (2007)

    Science.gov (United States)

    Fakhri, H.; Dehghani, A.

    2008-04-01

    We call attention to the misconstructions in a paper recently published in this journal [A. Chenaghlou and O. Faizy, J. Math. Phys. 48, 112106 (2007)]. It is shown that the constructed Barut-Girardello coherent states are problematic from the view points of the definition and the measure. The claimed coherencies for the Kravchuk functions cannot actually exist.

  9. Some comments on the paper: Controllability of fractional neutral stochastic functional differential systems, Z. Angew. Math. Phys. 65 (2014), no. 5, 941-959

    Science.gov (United States)

    Pierri, Michelle; O'Regan, Donal

    2016-04-01

    The abstract results and applications presented in "Controllability of fractional neutral stochastic functional differential systems, Z. Angew. Math. Phys. 65 (2014), no. 5, 941-959, are not correct. Moreover, the class of differential control problems studied in [1] is not H-controllable.

  10. Comments on the paper: Synthesis growth and characterization of copper mercury thiocyanate crystal [Indian J Pure & App Phys 49 (2011) 340-343

    OpenAIRE

    Srinivasan, Bikshandarkoil R.

    2015-01-01

    The unit cell parameters, infrared and UV-Vis spectral data reported in the paper by Vijayabhaskaran et al (Indian J Pure & App Phys 49 (2011) 340-343) cannot belong to the colourless crystalline compound formulated as copper mercury thiocyanate CuHg(SCN)4 as claimed by the authors.

  11. Erratum to ;Neutron-19C scattering: Emergence of universal properties in a finite range potential; [Phys. Lett. B 764 (2017) 196

    Science.gov (United States)

    Shalchi, M. A.; Yamashita, M. T.; Hadizadeh, M. R.; Frederico, T.; Tomio, Lauro

    2017-08-01

    Numerical results for the function (1 -EK /E0) kcot ⁡δ0R, as given in Phys. Lett. B 764 (2017) 196, are revised. Fig. 2 and Tables 2 and 3 should be replaced by the following corresponding figure and tables. The conclusions of the original paper remain unchanged.

  12. Erratum to “Neutron–19C scattering: Emergence of universal properties in a finite range potential” [Phys. Lett. B 764 (2017 196

    Directory of Open Access Journals (Sweden)

    M.A. Shalchi

    2017-08-01

    Full Text Available Numerical results for the function (1−EK/E0kcot⁡δ0R, as given in Phys. Lett. B 764 (2017 196, are revised. Fig. 2 and Tables 2 and 3 should be replaced by the following corresponding figure and tables. The conclusions of the original paper remain unchanged.

  13. Comment on "Rethinking first-principles electron transport theories with projection operators: The problems caused by partitioning the basis set" [J. Chem. Phys. 139, 114104 (2013)

    DEFF Research Database (Denmark)

    Brandbyge, Mads

    2014-01-01

    In a recent paper Reuter and Harrison [J. Chem. Phys.139, 114104 (2013)] question the widely used mean-field electron transport theories, which employ nonorthogonal localized basis sets. They claim these can violate an “implicit decoupling assumption,” leading to wrong results for the current...

  14. On the theory of MHD modes driven by strong ExB velocity shear in tokamaks. Addendum. 2000 Plasma Phys. Control. Fusion 42 57

    Energy Technology Data Exchange (ETDEWEB)

    Mikhailovskii, A.B.; Sharapov, S.E.; Timofeev, A.V. [JET Joint Undertaking, Abingdon, Oxfordshire OX14 3EA (United Kingdom)

    2000-07-01

    The theory of MHD modes driven by strong ExB velocity shear in tokamaks given by Mikhailovskii and Sharapov (2000 Plasma Phys. Control. Fusion 42 57) is revised. It is suggested that, in the approximations taken by these authors, there are no MHD eigenmodes if the cross-field velocity shear is larger then the Alfven frequency shear. (author)

  15. Overlapping cis sites used for splicing of HIV-1 env/nef and rev mRNAs.

    Science.gov (United States)

    Swanson, A K; Stoltzfus, C M

    1998-12-18

    Alternative splicing is used to generate more than 30 human immunodeficiency virus type 1 (HIV-1) spliced and unspliced mRNAs from a single primary transcript. The abundance of HIV-1 mRNAs is determined by the efficiencies with which its different 5' and 3' splice sites are used. Three splice sites (A4c, A4a, and A4b) are upstream of the rev initiator AUG. RNAs spliced at A4c, A4a, and A4b are used as mRNAs for Rev. Another 3' splice site (A5) is immediately downstream of the rev initiator. RNAs spliced at A5 are used as mRNAs for Env and Nef. In this report, primer extension analysis of splicing intermediates was used to show that there are eight branch points in this region, all of which map to adenosine residues. In addition, cis elements recognized by the cellular splicing machinery overlap; the two most 3' branch points overlap with the AG dinucleotides at rev 3' splice sites A4a and A4b. Competition of the overlapping cis sites for different splicing factors may play a role in maintaining the appropriate balance of mRNAs in HIV-1-infected cells. In support of this possibility, mutations at rev 3' splice site A4b AG dinucleotide dramatically increased splicing of the env/nef 3' splice site A5. This correlated with increased usage of the four most 3' branch points, which include those within the rev 3' splice site AG dinucleotides. Consistent with these results, analysis of a mutant in which three of the four env/nef branch points were inactivated indicated that use of splice site A5 was inhibited and splicing was shifted predominantly to the most 5' rev 3' splice site A4c with preferential use of the two most 5' branch points. Our results suggest that spliceosomes formed at rev A4a-4b, rev A4c, and env/nef A5 3' splice sites each recognize different subsets of the eight branch point sequences.

  16. Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expression

    Directory of Open Access Journals (Sweden)

    Jeang Kuan-Teh

    2011-07-01

    Full Text Available Abstract Post-transcriptional regulation of HIV-1 gene expression is mediated by interactions between viral transcripts and viral/cellular proteins. For HIV-1, post-transcriptional nuclear control allows for the export of intron-containing RNAs which are normally retained in the nucleus. Specific signals on the viral RNAs, such as instability sequences (INS and Rev responsive element (RRE, are binding sites for viral and cellular factors that serve to regulate RNA-export. The HIV-1 encoded viral Rev protein binds to the RRE found on unspliced and incompletely spliced viral RNAs. Binding by Rev directs the export of these RNAs from the nucleus to the cytoplasm. Previously, Rev co-factors have been found to include cellular factors such as CRM1, DDX3, PIMT and others. In this work, the nuclear matrix protein Matrin 3 is shown to bind Rev/RRE-containing viral RNA. This binding interaction stabilizes unspliced and partially spliced HIV-1 transcripts leading to increased cytoplasmic expression of these viral RNAs.

  17. Development of a real-time quantitative RT-PCR to detect REV contamination in live vaccine.

    Science.gov (United States)

    Luan, Huaibiao; Wang, Yixin; Li, Yang; Cui, Zhizhong; Chang, Shuang; Zhao, Peng

    2016-09-01

    Based on the published Avian reticuloendotheliosis virus (REV) whole genome sequence, primers and TaqMan probes were designed and synthesized, and the TaqMan probe fluorescence real-time quantitative RT-PCR (qRT-PCR) method for detecting the REV pol gene was established by optimizing the reaction conditions. Sensitivity analysis showed that the qRT-PCR method had a sensitivity that was 1,000-fold higher than conventional PCR. Additionally, no amplification signals were obtained when we attempted to detect DNA or cDNA of ALV-A/B/J, MDV, CIAV, IBDV, ARV, NDV, AIV, or other viruses, suggesting a high specificity for our method. Various titers of REV were artificially "spiked" into the FPV and MDV vaccines to simulate REV contamination in attenuated vaccines to validate this qRT-PCR method. Our findings indicated that this qRT-PCR method could detect REV contamination at a dose of 1 TCID50/1,000 feathers, which was 10,000-fold more sensitive than the regular RT-PCR detection (10(4) TCID50/1000 feathers).

  18. 一种CDMA EV-DO revA网络性能优化方案的设计%A Kind of Paln Design about Network Property Mayorization Based on CDMA EV-DO Rev A

    Institute of Scientific and Technical Information of China (English)

    李晓堂; 湛邵斌; 谢忠时

    2010-01-01

    CDMA EV-DO Rev.A作为CDMA EV-DO Rel.O技术之后的下一代版本极大地增强了对实时业务的支持.CDMA EV-DO Rev.A除了继续提高数据业务的带宽之外,引入了一系列关键技术,保证各种业务流获得其相应的业务质量保证.提供了一种针对CDMA EV-DO Rev.A QoS算法进行的优化设计方案,以保证更优的网络性能.

  19. Numerical Modelling of Large-Diameter Steel Piles at Horns Rev

    DEFF Research Database (Denmark)

    Augustesen, Anders Hust; Brødbæk, K. T.; Møller, M.;

    2009-01-01

    Today large-diameter monopiles are the most common foundation type used for large offshore wind farms. This paper aims to investigate the behaviour of monopiles under monotonic loading taking the interaction between the pile and the subsoil into account. Focus is paid to a monopile used as founda......Today large-diameter monopiles are the most common foundation type used for large offshore wind farms. This paper aims to investigate the behaviour of monopiles under monotonic loading taking the interaction between the pile and the subsoil into account. Focus is paid to a monopile used...... as foundation for a wind turbine at Horns Rev located in the Danish sector of the North Sea. The outer diameter of the pile is 4 m and the subsoil at the location consists primarily of sand. The behaviour of the pile is investigated under realistic loading conditions by means of a traditional Winkler...

  20. Update to Rev6 ignition designs NIF, with details about support tent in particular

    Science.gov (United States)

    Haan, S. W.; Berzak Hopkins, L.; Clark, D. S.; Eder, D.; Hammel, B. A.; Hamza, A.; Ho, D.; Jones, O. S.; Kritcher, A.; Lafortune, K.; MacGowan, B. J.; Meezan, N. B.; Milovich, J.; Peterson, J. L.; Robey, H. F.; Salmonson, J. D.; Spears, B. K.; Town, R. P.; Kline, J. L.; Wilson, D. C.; Simakov, A. N.; Yi, S. A.; Nikroo, A.; Huang, H.; Hoover, D.

    2013-10-01

    Ignition experiments on the National Ignition Facility will use an indirectly driven spherical implosion to assemble and ignite a mass of DT fuel. Requirements describing the specifics of the experiment and the corresponding expected performance were established several years prior. These requirements include laser features, target fabrication and characterization, and data obtained from pre-ignition experiments. Since those requirements were originally set, various NIF experiments using surrogate targets have motivated updates to the target designs and requirements. A summary of these updates will be presented. Rev6 designs for CH(Si), C(W), and Be(Cu) will be summarized. One particularly significant change regards the thickness of the tent films supporting the capsule, and the presentation will include updated thickness goals and the experimental motivation for the change. Prepared by LLNL under Contract DE-AC52-07NA27344.

  1. OPERATIONAL CIRCULAR No. 4 (REV. 1) – USE OF VEHICLES BELONGING TO OR RENTED BY CERN

    CERN Multimedia

    2012-01-01

    Operational Circular No. 4 (Rev. 1) entitled “Use of vehicles belonging to or rented by CERN”, approved by the Director-general following discussion in the Standing Concertation Committee meeting of 15 February 2012, is available on the intranet site of the Human Resources Department: https://hr-docs.web.cern.ch/hr-docs/opcirc/opcirc.asp It cancels and replaces Operational Circular No. 4 entitled “Conditions for use by members of the CERN personnel of vehicles belonging to or rented by CERN” of April 2003. This new version enables, in particular, to include CERN contractors and their personnel, to harmonize the structure of the circular with other circulars and to simplify the procedures by permitting electronics forms. Department Head Office HR Department

  2. Administrative Circulars Nos. 12 A and B (Rev. 1) – Education Fees

    CERN Multimedia

    HR Department

    2007-01-01

    Administrative Circulars Nos. 12 A and B (Rev. 1) – "Education Fees" are now available on the intranet site of the Human Resources Department: http://https://hr-docs.web.cern.ch/hr-docs/admincirc/admincirc_fr.asp These circulars cancel and replace Administrative Circular No.12 – "Education Grant" of April 1981. Copies will shortly be available in Departmental secretariats. Administrative Circular No. 12 A is applicable to staff members (except former "local staff"), fellows and paid associates, recruited before 1st January 2007. It may be noted that, at the initiative of the Human Resources Department, a number of important simplifications have been introduced. These cover, in particular, lump sum payments to compensate for accommodation, meals and journey expenses. Administrative Circular No. 12 B is applicable to staff members, fellows, scientific associates recruited as of 1st January 2007, as well as to former "local staff" recruited prior to this date. If you requi...

  3. Administrative Circulars Nos. 12 A and B (Rev. 1) – Education Fees

    CERN Multimedia

    HR Department

    2007-01-01

    Administrative Circulars Nos. 12 A and B (Rev. 1) – "Education Fees" are now available on the intranet site of the Human Resources Department: http://cern.ch/hr-docs/admincirc/admincirc.asp These Circulars cancel and replace Administrative Circular No.12 – "Education Grant" of April 1981. Copies will shortly be available in Departmental secretariats. Administrative Circular No. 12 A is applicable to staff members (except former "local staff"), fellows and paid associates, recruited before 1st January 2007. It may be noted that, at the initiative of the Human Resources Department, a number of important simplifications have been introduced. These cover in particular lump sum payments to compensate for accommodation, meals and journey expenses. Administrative Circular No. 12 B is applicable to staff members, fellows, scientific associates recruited as of 1st January 2007, as well as to former "local staff" recruited prior to this date. If you require any additional informa...

  4. Ensemble-based forecasting at Horns Rev: Ensemble conversion and kernel dressing

    DEFF Research Database (Denmark)

    Pinson, Pierre; Madsen, Henrik

    methodology. In a first stage, ensemble forecasts of meteorological variables are converted to power through a suitable power curve model. The relevance and benefits of employing a newly developed orthogonal fitting method for the power curve model over the traditional least-squares one are discussed...... predictive distributions. Such a methodology has the benefit of yielding predictive distributions that are of increased reliability (in a probabilistic sense) in comparison with the raw ensemble forecasts, while taking advantage of their high resolution....... of probabilistic forecasts, the resolution of which may be maximized by using meteorological ensemble predictions as input. The paper concentrates on the test case of the Horns Rev wind farm over a period of approximately one year, in order to describe, apply and discuss a complete ensemble-based forecasting...

  5. Indledende undersøgelser af Wave Star lokaliteten ved Horns Rev

    DEFF Research Database (Denmark)

    Kramer, Morten

    Havområdet ved Horns Rev havvindmøllepark er, pga. tilslutningsmulighederne for el, egnet til udbygning med et eller flere demonstrationsanlæg baseret på bølgekraft. Nærværende rapport beskriver detaljerede undersøgelser vedr. placering af den næste skala 1:2 maskine af Wave Star på lokaliteten....... Formålet med nærværende skrift er at beskrive bølgerne på lokaliteten mht. bølgehøjde, -periode og -retning, at estimere årsproduktionen fra en skala 1:2 Wave Star maskine og forventet orientering af maskinen for maksimal årsproduktion samt at vurdere de største bølger i samspil med vandstand som kan...

  6. HuR cytoplasmic expression is associated with increased cyclin A expression and poor outcome with upper urinary tract urothelial carcinoma

    Directory of Open Access Journals (Sweden)

    Liang Peir-In

    2012-12-01

    Full Text Available Abstract Background HuR is an RNA-binding protein that post-transcriptionally modulates the expressions of various target genes implicated in carcinogenesis, such as CCNA2 encoding cyclin A. No prior study attempted to evaluate the significance of HuR expression in a large cohort with upper urinary tract urothelial carcinomas (UTUCs. Methods In total, 340 cases of primary localized UTUC without previous or concordant bladder carcinoma were selected. All of these patients received ureterectomy or radical nephroureterectomy with curative intents. Pathological slides were reviewed, and clinical findings were collected. Immunostaining for HuR and cyclin A was performed and evaluated by using H-score. The results of cytoplasmic HuR and nuclear cyclin A expressions were correlated with disease-specific survival (DSS, metastasis-free survival (MeFS, urinary bladder recurrence-free survival (UBRFS, and various clinicopathological factors. Results HuR cytoplasmic expression was significantly related to the pT status, lymph node metastasis, a higher histological grade, the pattern of invasion, vascular and perineurial invasion, and cyclin A expression (p = 0.005. Importantly, HuR cytoplasmic expression was strongly associated with a worse DSS (p p p = 0.0370 in the univariate analysis, and the first two results remained independently predictive of adverse outcomes (p = 0.038, relative risk [RR] = 1.996 for DSS; p = 0.027, RR = 1.880 for MeFS. Cyclin A nuclear expression was associated with a poor DSS (p = 0.0035 and MeFS (p = 0.0015 in the univariate analysis but was not prognosticatory in the multivariate analyses. High-risk patients (pT3 or pT4 with/without nodal metastasis with high HuR cytoplasmic expression had better DSS if adjuvant chemotherapy was performed (p = 0.015. Conclusions HuR cytoplasmic expression was correlated with adverse phenotypes and cyclin A overexpression and also independently predictive

  7. Bayesian Assessment of Accuracy Properties of Rose Bengal Test in REV-1 Vaccinated Small Ruminants

    Directory of Open Access Journals (Sweden)

    KUJTIM MERSINAJ

    2015-12-01

    Full Text Available The live Brucella melitensis Rev-1 strain is considered the best vaccine available for the prevention of brucellosis in small ruminants. In Albania, vaccination of small ruminants with REV-1 has been used under various regimes since 2004. The effectiveness of the vaccination campaigns is monitored through a post vaccination monitoring and surveillance system based on testing vaccinated animals with Rose Bengal Plate Test (RBPT. Regrettably the test accuracy properties such as the sensitivity and specificity have not been validated in vaccinated small ruminants. The lack of knowledge on these properties hampers the correct evaluation of the true sero-conversion rate of the post vaccination monitoring at national and at flock scale. This study addressed this issue by using a Bayesian modelling framework to estimate two serological tests the RBPT which is the standard serological test used in the post vaccination monitoring and Complement Fixation Test (CFT. Serum samples from 191 reportedly vaccinated small ruminants were tested in parallel with RBPT and CFT. The estimates of sensitivity and specificity values of RBPT were 91% (95% CrI: 82 -98 and 89% (95% CrI: 70 - 98. For CFT the sensitivity resulted 86% (95% CrI: 70 - 95 and the specificity 95% (95% CrI: 80 - 99. The good sensitivity and acceptable specificity of RBPT support its utilization as screening test for post vaccination monitoring. The interpretation of the post vaccination data with RBPT are well acceptable at national level but not well suited for flock status interpretation. In terms of disease diagnosis, especially for a latter phase of the brucellosis control strategy in Albania, both tests could justify their use in association.

  8. A new human cholangiocellular carcinoma cell line (HuCC-T1) producing carbohydrate antigen 19/9 in serum-free medium.

    Science.gov (United States)

    Miyagiwa, M; Ichida, T; Tokiwa, T; Sato, J; Sasaki, H

    1989-06-01

    A human cholangiocellular carcinoma cell line, HuCC-T1, was established in vitro from the malignant cells of ascites of a 56-yr-old patient. Histologic findings of the primary liver tumor revealed a moderately differentiated adenocarcinoma. Tumor cells from the ascites have been cultured with RPMI 1640 medium containing 0.2% lactalbumin hydrolysate and the cultured cells grew as monolayers with a population doubling time of 74 h during exponential growth at Passage 25. They had an epithelial-like morphology and were positive for mucine staining. Ultrastructural studies revealed the presence of microvilli on the cell surface and poorly developed organelles in the cytoplasm. The HuCC-T1 cell was tumorigenic in nude mice. The number of chromosomes in HuCC-T1 ranged from 61 to 80. These human cholangiocellular carcinoma cells in serum-free medium secreted several tumor markers, including carbohydrate antigen 19/9, carbohydrate antigen 125, carcinoembryonic antigen, and tissue polypeptide antigen. The carbohydrate antigen 19/9 secretion level of HuCC-T1 cells cultured in RPMI 1640 medium with 1% fetal bovine serum was sixfold higher than that with 0.2% lactalbumin hydrolysate. These findings suggest that HuCC-T1 will provide useful information to clarify the mechanism of tumor marker secretion and tumor cell growth in the human cholangiocellular carcinoma.

  9. Comparison between two treatment protocols with recombinant Human Erythropoietin (rHuEpo in the treatment of late anemia in neonates with Rh-Isoimmunization

    Directory of Open Access Journals (Sweden)

    A.A. Zuppa

    2012-08-01

    Full Text Available Objectve. The Rh-hemolytic disease can lead to a late anemia by hemolytic and hyporigenerative mechanism. We compared the effectiveness of rHuEPO in two care protocols that differ for doses of rHuEPO administrated and for timing of administration. Methods. A cohort of 14 neonates was investigated. The neonates were treated with two different protocols. Protocol A: a dose of 200 U/kg/day of rHuEpo administered subcutaneously starting from the end of the second week of life; Protocol B: a dose of 400 U/kg/day of rHuEpo administered subcutaneously starting from the end of the first week of life. Results. The hematocrit values in the protocol A group decreased during treatment (32,5% vs 25,2%, whereas the hematocrit value in protocol B group remained almost stable (38,7% vs 42,8%. The mean numbers of platelets remained stable in both groups while neutrophils increased in protocol A group and decreased in protocol B (p<0,05. Reticulocyte count increased during treatment in both groups, although only in protocol B group it was statistically significative (p<0,05. Conclusions. Our results suggest a similar efficacy between the two treatment protocols. Increasing doses of rHuEPO do not seem enhancing their effectiveness and the incidence of side effects.

  10. Cellular ability to sense spatial gradients in the presence of multiple competitive ligands

    Science.gov (United States)

    Liou, Shu-Hao; Chen, Chia-Chu

    2012-01-01

    Many eukaryotic and prokaryotic cells can exhibit remarkable sensing ability under small gradients of chemical compounds. In this study, we approach this phenomenon by considering the contribution of multiple ligands to the chemical kinetics within the Michaelis-Menten model. This work was inspired by the recent theoretical findings of Hu [Phys. Rev. Lett.PRLTAO0031-900710.1103/PhysRevLett.105.048104 105, 048104 (2010)]. Our treatment with practical binding energies and chemical potentials provides results that are consistent with experimental observations.

  11. Pharmacological Progress of HuZhang%中药虎杖药理作用研究进展

    Institute of Scientific and Technical Information of China (English)

    黄海量

    2012-01-01

    总结近年来关于中药虎杖中有效成分治疗心血管系统、消化系统、呼吸系统、内分泌系统、生殖系统、免疫系统等疾病的文献报道,发现虎杖含有的丰富活性成分具有多项药理作用,临床应用日益广泛,但其毒理研究和有效成分提取方法的报道鲜见.%Literature and reports on effective constituents of HuZhang (Polygonum cuspidatum) in treating the diseases of cardiovascular system, digestive system, respiratory system, endocrine system, reproductive system, immune system and so on were concluded in recent years and the result of study showed that rich active constituents in HuZhang had pharmacological functions and were widely used day by day, but toxicological studies and extraction of effective constituent were rarely reported.

  12. Optimization and risk analyses for rule curves of reservoir operation: application to Tien-Hua-Hu Reservoir in Taiwan.

    Science.gov (United States)

    Kuo, J T; Hsu, N S; Chiu, S K

    2006-01-01

    Tien-Hua-Hu Reservoir is currently under planning by the Water Resources Agency, Taiwan to meet the increasing water demands of central Taiwan arising from rapid growth of domestic water supply, and high-tech industrial parks. This study develops a simulation model for the ten-day period reservoir operation to calculate the ten-day water shortage index under varying rule curves. A genetic algorithm is coupled to the simulation model to find the optimal rule curves using the minimum ten-day water shortage index as an objective function. This study generates many sets of synthetic streamflows for risk, reliability, resiliency, and vulnerability analyses of reservoir operation. ARMA and disaggregation models are developed and applied to the synthetic streamflow generation. The optimal rule curves obtained from this study perform better in the ten-day shortage index when compared to the originally designed rule curves from a previous study. The optimal rule curves are also superior to the originally designed rule curves in terms of vulnerability. However, in terms of reliability and resiliency, the optimal rule curves are inferior to the those originally designed. Results from this study have provided in general a set of improved rule curves for operation of the Tien-Hua-Hu Reservoir. Furthermore, results from reliability, resiliency and vulnerability analyses offer much useful information for decision making in reservoir operation.

  13. r-huEPO在胃肠道恶性肿瘤患者围手术期应用

    Institute of Scientific and Technical Information of China (English)

    缪传文; 李继坤; 温玉刚; 周立生; 李旭

    2006-01-01

    胃肠道恶性肿瘤往往伴有程度不等的贫血,以往临床上除了输血疗效确切外,只有使用各种生血制剂,主要是铁剂,往往起效缓慢,难以令人满意。而输血由于血源紧张,还有各种输血的并发症,及传播各种严重传染病性疾病的危险,使用受到限制。重组人促红细胞生成素(recombinant erythropoietin)的围手术期红细胞动员也越来越受到临床重视,有研究表现大剂量应用r-huEPO有明显的红细胞动员作用。2003年-2005年,我们应用r-huEPO治疗胃肠道恶性肿瘤伴贫血的手术患者276例,取得了较好的疗效。

  14. Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell

    Directory of Open Access Journals (Sweden)

    Aris Haryanto

    2015-10-01

    Full Text Available The application of fluorescent proteins as expression markers and protein fusion partners has provedimmensely valuable for resolving the organization of biological events in living cells. EGFP and DsRed2 arecommonly fluorescent marker protein which is used for biotechnology and cell biology research. The presentstudy was designed to identify the expression vector that suitable to ligate with DNA encoding HBV coreprotein for intracellular localization study in hepatocyte cell, which were expressed as fusion proteins. We alsocompared and quantified the expressed fluorescent protein which predominantly localized in the cellcompartment. The results indicated that DsRed2 shown as less than ideal for intracellular localization study ofthan EGFP, because of its tetrameric structure of the fluorescent protein and when fused to a protein of interest,the fusion protein often forms aggregates in the living cells. In contrast, EGFP fluorescent protein shown a muchhigher proportion of cytoplasmic localization, thus being more suitable for analysis of intracellular localizationthan DsRed2 fluorescent protein. EGFP fluorescent protein is also capable to produce a strong green fluorescencewhen excited by blue light, without any exogenously added substrate or cofactor, events inside living cell canthus be visualized in a non-invasive way. Based on our present quantitative data and some reasons above shownthat EGFP is more suitable than DsRed2 as a fluorescent marker protein for intracellular localization study intoHuH-7 cell.Keywords: EGFP, DsRed2 fluorescent protein , HuH-7 cell, HBV, intracellular localization

  15. Role of AtPolζ, AtRev1, and AtPolη in UV light-induced mutagenesis in Arabidopsis.

    Science.gov (United States)

    Nakagawa, Mayu; Takahashi, Shinya; Tanaka, Atsushi; Narumi, Issay; Sakamoto, Ayako N

    2011-01-01

    Translesion synthesis (TLS) is a DNA damage tolerance mechanism in which DNA lesions are bypassed by specific polymerases. To investigate the role of TLS activities in ultraviolet light-induced somatic mutations, we analyzed Arabidopsis (Arabidopsis thaliana) disruptants of AtREV3, AtREV1, and/or AtPOLH genes that encode TLS-type polymerases. The mutation frequency in rev3-1 or rev1-1 mutants decreased compared with that in the wild type, suggesting that AtPolζ and AtRev1 perform mutagenic bypass events, whereas the mutation frequency in the polh-1 mutant increased, suggesting that AtPolη performs nonmutagenic bypass events with respect to ultraviolet light-induced lesions. The rev3-1 rev1-1 double mutant showed almost the same mutation frequency as the rev1-1 single mutant. The increased mutation frequency found in polh-1 was completely suppressed in the rev3-1 polh-1 double mutant, indicating that AtPolζ is responsible for the increased mutations found in polh-1. In summary, these results suggest that AtPolζ and AtRev1 are involved in the same (error-prone) TLS pathway that is independent from the other (error-free) TLS pathway mediated by AtPolη.

  16. Administrative Circular No. 30 (Rev. 3) - “Financial benefits on taking up appointment and on termination of contract”

    CERN Multimedia

    2013-01-01

    Administrative Circular No. 30 (Rev. 3) entitled “Financial benefits on taking up appointment and on termination of contract”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 27 June 2013 and entering into force on 1 August 2013, is available on the intranet site of the Human Resources Department (see here).   Administrative Circular No. 30 (Rev. 3) is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 30 (Rev. 2/Corr.) entitled "Financial benefits on taking up appointment and on termination of contract” of September 2009. This circular was revised in order to implement the modifications introduced into the Staff Rules and Regulations in January 2013 relating to the introduction of the status of Associate Member States and new categories of associated members of the personnel. In particular, the notion of “Member State” in Annexe II (&a...

  17. Administrative Circular No. 30 (Rev. 3) - “Financial benefits on taking up appointment and on termination of contract”

    CERN Multimedia

    2013-01-01

    Administrative Circular No. 30 (Rev. 3) entitled “Financial benefits on taking up appointment and on termination of contract”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 27 June 2013 and entering into force on 1 August 2013, is available on the intranet site of the Human Resources Department (see here).   Administrative Circular No. 30 (Rev. 3) is applicable to all members of the personnel. It cancels and replaces Administrative Circular No. 30 (Rev. 2/Corr.) entitled "Financial benefits on taking up appointment and on termination of contract” of September 2009. This circular was revised in order to implement the modifications introduced into the Staff Rules and Regulations in January 2013 relating to the introduction of the status of Associate Member States and new categories of associated members of the personnel. In particular, the notion of “Member State” in Annexe II (&a...

  18. Protection against brucellosis in goats, five years after vaccination with reduced-dose Brucella melitensis Rev 1 vaccine.

    Science.gov (United States)

    Díaz-Aparicio, E; Hernández, L; Suárez-Güemes, F

    2004-02-01

    The protection conferred by the reduced-dose Rev 1 Brucella melitensis vaccine in goats that had been immunized 5 years previously was evaluated. Sixteen goats vaccinated 5 years before with Rev 1 (1 x 10(5) cfu) and 5 non-vaccinated goats were challenged with B. melitensis 16M (4 x 10(5) cfu) using the conjunctival route. After giving birth or aborting, the goats were sacrificed and tissue samples were taken for bacteriological study. The challenge strain was recovered in 12%, of the animals from the vaccinated group, and in (80% of the control group. It is concluded, therefore that the use of reduced-dose Rev 1 protects goats vaccinated in endemic areas for at least 5 years after immunization.

  19. Administrative Circular No. 2 (Rev. 7) - Recruitment, appointment and possible developments regarding the contractual position of staff members

    CERN Multimedia

    2015-01-01

    Administrative Circular No. 2 (Rev. 7), entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members", approved by the Director-General following discussion at the Standing Concertation Committee meeting held on 17 February 2015 is available via the following link: AC No. 2 (Rev.7).   This revised circular cancels and replaces Administrative Circular No. 2 (Rev. 6), entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members" and dated January 2015. The circular was revised in order to implement the amendment to Article R II 1.17 of the Staff Regulations, which introduces the possibility of extending limited-duration (LD) contracts up to a maximum total duration of eight years from the previous duration of five years. The award of indefinite contracts will continue to be subject to the outcome of a competitive process. Department Head Of...

  20. Mouse monocyte/macrophage cell line J774A.1 migration induced by the activation of CB1 depends on HuR%激活大麻素受体1诱导的单核巨噬细胞J774A.1的迁移依赖RNA结合蛋白HuR

    Institute of Scientific and Technical Information of China (English)

    赵中新; 常娜; 盖菁菁; 田蕾; 李丽英

    2016-01-01

    目的 研究大麻素受体1(cannabinoid receptor 1,CB1)在单核巨噬细胞迁移中的重要作用以及RNA结合蛋白人抗原R (human antigen R,HuR)参与其中的可能机制.方法 选用单核巨噬细胞系J774A.1,应用琼脂糖凝胶电泳和免疫荧光染色技术鉴定J774A.1中CB1以及HuR的表达;ACEA和AM281分别为CB1的药理学激动剂和拮抗剂,应用Boyden chamber法检测ACEA和AM281对J774A.1迁移活性的影响.HuR的基因干扰用于确定激活CB1诱导的J774A.1迁移功能是否依赖HuR;胞质蛋白的分离用于探究激活CB1是否能引起J774A.1胞质中HuR的富集;RT-qPCR和Western blotting法检测CB1和HuR mRNA和蛋白质的变化情况.结果 该研究证明J774A.1在基因和蛋白质水平上均表达CB1和HuR;激活CB1能够促进J774A.1的迁移(P<0.01)并且能够被其药理学拮抗剂AM281所抑制;激活CB1诱导的J774A.1的迁移依赖HuR;激活CB1促进了J774A.1胞质中HuR的富集进一步影响了CB1的表达,由此HuR参与了激活CB1诱导的J774A.1的迁移.结论 激活CB1能够诱导单核巨噬细胞系J774A.1的迁移,且此过程依赖RNA结合蛋白HuR.

  1. Temperature inducible β-sheet structure in the transactivation domains of retroviral regulatory proteins of the Rev family

    Science.gov (United States)

    Thumb, Werner; Graf, Christine; Parslow, Tristram; Schneider, Rainer; Auer, Manfred

    1999-11-01

    The interaction of the human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev with cellular cofactors is crucial for the viral life cycle. The HIV-1 Rev transactivation domain is functionally interchangeable with analog regions of Rev proteins of other retroviruses suggesting common folding patterns. In order to obtain experimental evidence for similar structural features mediating protein-protein contacts we investigated activation domain peptides from HIV-1, HIV-2, VISNA virus, feline immunodeficiency virus (FIV) and equine infectious anemia virus (EIAV) by CD spectroscopy, secondary structure prediction and sequence analysis. Although different in polarity and hydrophobicity, all peptides showed a similar behavior with respect to solution conformation, concentration dependence and variations in ionic strength and pH. Temperature studies revealed an unusual induction of β-structure with rising temperatures in all activation domain peptides. The high stability of β-structure in this region was demonstrated in three different peptides of the activation domain of HIV-1 Rev in solutions containing 40% hexafluoropropanol, a reagent usually known to induce α-helix into amino acid sequences. Sequence alignments revealed similarities between the polar effector domains from FIV and EIAV and the leucine rich (hydrophobic) effector domains found in HIV-1, HIV-2 and VISNA. Studies on activation domain peptides of two dominant negative HIV-1 Rev mutants, M10 and M32, pointed towards different reasons for the biological behavior. Whereas the peptide containing the M10 mutation (L 78E 79→D 78L 79) showed wild-type structure, the M32 mutant peptide (L 78L 81L 83→A 78A 81A 83) revealed a different protein fold to be the reason for the disturbed binding to cellular cofactors. From our data, we conclude, that the activation domain of Rev proteins from different viral origins adopt a similar fold and that a β-structural element is involved in binding to a

  2. Administrative circular No. 2 (Rev. 5) – Recruitment, appointment and possible developments regarding the contractual position of staff members

    CERN Multimedia

    HR Department

    2011-01-01

    Administrative Circular No. 2 (Rev. 5) entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 1 September 2011, is available on the intranet site of the Human Resources Department: https://cern.ch/hr-docs/admincirc/admincirc.asp It cancels and replaces Administrative Circular No. 2 (Rev. 4) entitled "Recruitment, appointment and possible developments regarding the contractual position of staff members" of September 2009. Department Head Office

  3. A stably expressed llama single-domain intrabody targeting Rev displays broad-spectrum anti-HIV activity.

    Science.gov (United States)

    Boons, Eline; Li, Guangdi; Vanstreels, Els; Vercruysse, Thomas; Pannecouque, Christophe; Vandamme, Anne-Mieke; Daelemans, Dirk

    2014-12-01

    The HIV Rev protein mediates the transport of partially and unspliced HIV mRNA from the nucleus to the cytoplasm. Rev multimerizes on a secondary stem-loop structure present in the viral intron-containing mRNA species and recruits the cellular karyopherin CRM1 to export viral mRNAs from the nucleus to the cytoplasm. Previously we have identified a single-domain intrabody (Nb(190)), derived from a llama heavy-chain antibody, which efficiently inhibits Rev multimerization and suppresses the production of infectious virus. We recently mapped the epitope of this nanobody and demonstrated that Rev residues K20 and Y23 are crucial for interaction while residues V16, H53 and L60 are important to a lesser extent. Here, we generated cell lines stably expressing Nb(190) and assessed the capacity of these cell lines to suppress the replication of different HIV-1 subtypes. These cells stably expressing the single-domain antibody are protected from virus-induced cytopathogenic effect even in the context of high multiplicity of infection. In addition, the replication of different subtypes of group M and one strain of group O is significantly suppressed in these cell lines. Next, we analysed the natural variations of Rev amino acids in sequence samples from HIV-1 infected patients worldwide and assessed the effect of Nb(190) on the most prevalent polymorphisms occurring at the key epitope positions (K20 and Y23) in Rev. We found that Nb(190) was able to suppress the function of these Rev variants except for the K20N mutant, which was present in only 0.7% of HIV-1 sequence populations (n = 4632). Cells stably expressing the single-domain intrabody Nb(190) are protected against virus-induced cytopathogenic effect and display a selective survival advantage upon infection. In addition, Nb(190) suppresses the replication of a wide range of different HIV-1 subtypes. Large-scale sequence analysis reveals that the Nb(190) epitope positions in Rev are well conserved across major HIV-1

  4. Reliability of Concentric, Eccentric and Isometric Knee Extension and Flexion when using the REV9000 Isokinetic Dynamometer

    DEFF Research Database (Denmark)

    de Carvalho Froufe Andrade, Alberto César Pereira; Caserotti, Paolo; de Carvalho, Carlos Manuel Pereira

    2013-01-01

    The aim of this study was to assess the reliability of isokinetic and ISO knee extensor and flexor muscle strength when using the REV9000 (Technogym) isokinetic dynamometer. Moreover, the reliability of several strength imbalance indices and bilateral ratios were also examined. Twenty......). The results of this study showed a high reproducibility in eccentric (0.95-0.97), concentric (0.95-0.96) and isometric (0.93-0.96), isokinetic strength for knee extensor and flexor muscles, thus indicating that the REV9000 isokinetic dynamometer can be used in future sports performance studies. A low...

  5. iNR-PhysChem: a sequence-based predictor for identifying nuclear receptors and their subfamilies via physical-chemical property matrix.

    Directory of Open Access Journals (Sweden)

    Xuan Xiao

    Full Text Available Nuclear receptors (NRs form a family of ligand-activated transcription factors that regulate a wide variety of biological processes, such as homeostasis, reproduction, development, and metabolism. Human genome contains 48 genes encoding NRs. These receptors have become one of the most important targets for therapeutic drug development. According to their different action mechanisms or functions, NRs have been classified into seven subfamilies. With the avalanche of protein sequences generated in the postgenomic age, we are facing the following challenging problems. Given an uncharacterized protein sequence, how can we identify whether it is a nuclear receptor? If it is, what subfamily it belongs to? To address these problems, we developed a predictor called iNR-PhysChem in which the protein samples were expressed by a novel mode of pseudo amino acid composition (PseAAC whose components were derived from a physical-chemical matrix via a series of auto-covariance and cross-covariance transformations. It was observed that the overall success rate achieved by iNR-PhysChem was over 98% in identifying NRs or non-NRs, and over 92% in identifying NRs among the following seven subfamilies: NR1--thyroid hormone like, NR2--HNF4-like, NR3--estrogen like, NR4--nerve growth factor IB-like, NR5--fushi tarazu-F1 like, NR6--germ cell nuclear factor like, and NR0--knirps like. These rates were derived by the jackknife tests on a stringent benchmark dataset in which none of protein sequences included has ≥60% pairwise sequence identity to any other in a same subset. As a user-friendly web-server, iNR-PhysChem is freely accessible to the public at either http://www.jci-bioinfo.cn/iNR-PhysChem or http://icpr.jci.edu.cn/bioinfo/iNR-PhysChem. Also a step-by-step guide is provided on how to use the web-server to get the desired results without the need to follow the complicated mathematics involved in developing the predictor. It is anticipated that iNR-PhysChem may

  6. Human-derived IgG level as an indicator for EBV-associated lymphoma model in Hu-PBL/SCID chimeras.

    Science.gov (United States)

    Tang, Yunlian; He, Rongfang; Zhang, Yang; Liu, Fang; Cheng, Ailan; Wu, Yimou; Gan, Runliang

    2011-05-09

    Epstein-Barr virus (EBV) has a close association with various types of human lymphomas. Animal models are essential to elucidate the pathogenesis of human EBV-associated lymphomas. The aim of the present study is to evaluate the association between human IgG concentration and EBV-associated lymphoma development in huPBL/SCID mice. Human peripheral blood lymphocytes (hu-PBL) from EBV-seropositive donors were inoculated intraperitoneally into SCID mouse. Immunohistochemical staining was used to examine differentiated antigens of tumor cells. EBV infection of the induced tumors was detected by in situ hybridization. IgG concentrations in the serums of 12 SCID mice were measured by unidirectional immunodiffusion assay. 21 out of 29 mice developed tumors in their body. Immunohistochemical staining showed that all induced tumors were LCA (leukocyte common antigen) positive, B-cell markers (CD20, CD79a) positive, and T-cell markers (both CD3 and CD45RO) negative. The tumors can be diagnosed as human B-cell lymphomas by these morphological and immunohistochemical features. In situ hybridization exhibited resultant tumor cells had EBV encoded small RNA-1 (EBER-1). Human-derived IgG could be found in the serum from SCID mice on the 15th day following hu-PBL transplantation, and IgG levels increased with the tumor development in 6 hu-PBL/SCID chimeras. Intraperitoneal transfer of hu-PBLs from EBV+ donors to SCID mice leads to high human IgG levels in mouse serum and B cell lymphomas. Our findings suggest that increasing levels of human-derived IgG in peripheral blood from hu-PBL/SCID mice could be used to monitor EBV-related human B-cell lymphoma development in experimental animals.

  7. Development and Characterization of a Humanized Anti-HER2 Antibody HuA21 with Potent Anti-Tumor Properties in Breast Cancer Cells.

    Science.gov (United States)

    Li, Ruilin; Hu, Siyi; Chang, Yan; Zhang, Zhihui; Zha, Zhao; Huang, Hui; Shen, Guodong; Liu, Jing; Song, Lihua; Wei, Wei

    2016-04-15

    Human epidermal growth factor receptor 2 (HER2) is one of the most studied tumor-associated antigens for cancer immunotherapy. An engineered anti-HER-2 chimeric A21 antibody (chA21) is a chimeric antibody targeted to subdomain I of the HER2 extracellular domain. Here, we report the anti-tumor activity of the novel engineered monoclonal antibody humanized chA21 (HuA21) that targets HER2 on the basis of chA21, and we describe the underlying mechanisms. Our results reveal that HuA21 markedly inhibits the proliferation and migration of HER2-overexpressing breast cancer cells and causes enhanced antibody-dependent cell-mediated cytotoxicity potency against HER2-overexpressing tumor cells. In particular, HuA21, but not trastuzumab (Tra), markedly suppresses growth and enhances the internalization of the antibody in Tra-resistant BT-474 breast cancer cells. These characteristics are highly associated with the intrinsic ability of HuA21 to down-regulate HER2 activation and inhibit the extracellular signal-regulated kinase 1/2 (ERK1/2) and protein kinase B (Akt) signaling pathways. Furthermore, the combination of HuA21 with Tra synergistically enhances the anti-tumor effects in vitro and in vivo and inhibits HER2 activation and the ERK1/2 and Akt signaling pathways. Altogether, our results suggest that HuA21 may represent a unique anti-HER2 antibody with potential as a therapeutic candidate alone or in combination with other anti-HER2 reagents in cancer therapy.

  8. Development and Characterization of a Humanized Anti-HER2 Antibody HuA21 with Potent Anti-Tumor Properties in Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Ruilin Li

    2016-04-01

    Full Text Available Human epidermal growth factor receptor 2 (HER2 is one of the most studied tumor-associated antigens for cancer immunotherapy. An engineered anti-HER-2 chimeric A21 antibody (chA21 is a chimeric antibody targeted to subdomain I of the HER2 extracellular domain. Here, we report the anti-tumor activity of the novel engineered monoclonal antibody humanized chA21 (HuA21 that targets HER2 on the basis of chA21, and we describe the underlying mechanisms. Our results reveal that HuA21 markedly inhibits the proliferation and migration of HER2-overexpressing breast cancer cells and causes enhanced antibody-dependent cell-mediated cytotoxicity potency against HER2-overexpressing tumor cells. In particular, HuA21, but not trastuzumab (Tra, markedly suppresses growth and enhances the internalization of the antibody in Tra-resistant BT-474 breast cancer cells. These characteristics are highly associated with the intrinsic ability of HuA21 to down-regulate HER2 activation and inhibit the extracellular signal-regulated kinase 1/2 (ERK1/2 and protein kinase B (Akt signaling pathways. Furthermore, the combination of HuA21 with Tra synergistically enhances the anti-tumor effects in vitro and in vivo and inhibits HER2 activation and the ERK1/2 and Akt signaling pathways. Altogether, our results suggest that HuA21 may represent a unique anti-HER2 antibody with potential as a therapeutic candidate alone or in combination with other anti-HER2 reagents in cancer therapy.

  9. HIV-1 Pathogenesis and Therapeutic Intervention in the SCID-hu Thy/Liv Mouse: A Model for Primary HIV-1 Infection in the Human Thymus

    OpenAIRE

    Su, Lishan

    1997-01-01

    The SCID-hu Thy/Liv mouse is a model for the analysis of human thymopoiesis. It has been constructed by engrafting fragments of human fetal liver and thymus into the immunodeficient C.B-17 scid/scid (SCID) mouse. The resulting ‘Thy/Liv’ organ promotes long-term differentiation of human T cells. Given the apparently normal physiology of the SCID-hu Thy/Liv organ, it has been used to explore the pathophysiologic mechanisms of HIV-1 infection in vivo, and to test therapeutic modalities such as a...

  10. HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-γc-/- (RAG-hu mouse model

    Directory of Open Access Journals (Sweden)

    Connick Elizabeth

    2006-11-01

    Full Text Available Abstract Background The currently well-established humanized mouse models, namely the hu-PBL-SCID and SCID-hu systems played an important role in HIV pathogenesis studies. However, despite many notable successes, several limitations still exist. They lack multi-lineage human hematopoiesis and a functional human immune system. These models primarily reflect an acute HIV infection with rapid CD4 T cell loss thus limiting pathogenesis studies to a short-term period. The new humanized Rag2-/-γc-/- mouse model (RAG-hu created by intrahepatic injection of CD34 hematopoietic stem cells sustains long-term multi-lineage human hematopoiesis and is capable of mounting immune responses. Thus, this model shows considerable promise to study long-term in vivo HIV infection and pathogenesis. Results Here we demonstrate that RAG-hu mice produce human cell types permissive to HIV-1 infection and that they can be productively infected by HIV-1 ex vivo. To assess the capacity of these mice to sustain long-term infection in vivo, they were infected by either X4-tropic or R5-tropic HIV-1. Viral infection was assessed by PCR, co-culture, and in situ hybridization. Our results show that both X4 and R5 viruses are capable of infecting RAG-hu mice and that viremia lasts for at least 30 weeks. Moreover, HIV-1 infection leads to CD4 T cell depletion in peripheral blood and thymus, thus mimicking key aspects of HIV-1 pathogenesis. Additionally, a chimeric HIV-1 NL4-3 virus expressing a GFP reporter, although capable of causing viremia, failed to show CD4 T cell depletion possibly due to attenuation. Conclusion The humanized RAG-hu mouse model, characterized by its capacity for sustained multi-lineage human hematopoiesis and immune response, can support productive HIV-1 infection. Both T cell and macrophage tropic HIV-1 strains can cause persistent infection of RAG-hu mice resulting in CD4 T cell loss. Prolonged viremia in the context of CD4 T cell depletion seen in this

  11. Response to Comment on "On Higher-Order Corrections to Gyrokinetic Vlasov-Poisson Equations in the Long Wavelength Limit [Phys. Plasmas 16, 044506 (2009)]"

    Energy Technology Data Exchange (ETDEWEB)

    W. W. Lee, and R. A. Kolesnikov

    2009-11-20

    We show in this Response that the nonlinear Poisson's equation in our original paper derived from the drift kinetic approach can be verified by using the nonlinear gyrokinetic Poisson's equation of Dubin et al. [Phys. Fluids 26, 3524 (1983)]. This nonlinear contribution in φ2 is indeed of the order of k4⊥ in the long wavelength limit and remains finite for zero ion temperature, in contrast to the nonlinear term by Parra and Catto [Plasma Phys. Control. Fusion 50, 065014 (2008)], which is of the order of k2⊥ and diverges for Ti → 0. For comparison, the leading term for the gyrokinetic Poisson's equation in this limit is of the order of k2⊥φ,

  12. Comment on “Maxwell's equations and electromagnetic Lagrangian density in fractional form” [J. Math. Phys. 53, 033505 (2012)

    Energy Technology Data Exchange (ETDEWEB)

    Rabei, Eqab M.; Al-Jamel, A.; Widyan, H. [Physics Department, Al al-Bayt University, Mafraq (Jordan); Baleanu, D. [Department of Chemical and Materials Engineering, Faculty of Engineering, King Abdulaziz University, Jeddah (Saudi Arabia); Department of Mathematics and Computer Sciences, Cankaya University, Ankara (Turkey); Institute of Space Sciences, Magurele-Bucharest (Romania)

    2014-03-15

    In a recent paper, Jaradat et al. [J. Math. Phys. 53, 033505 (2012)] have presented the fractional form of the electromagnetic Lagrangian density within the Riemann-Liouville fractional derivative. They claimed that the Agrawal procedure [O. P. Agrawal, J. Math. Anal. Appl. 272, 368 (2002)] is used to obtain Maxwell's equations in the fractional form, and the Hamilton's equations of motion together with the conserved quantities obtained from fractional Noether's theorem are reported. In this comment, we draw the attention that there are some serious steps of the procedure used in their work are not applicable even though their final results are correct. Their work should have been done based on a formulation as reported by Baleanu and Muslih [Phys. Scr. 72, 119 (2005)].

  13. Erratum: ``Whittaker pairs for the Virasoro algebra and the Gaiotto-Bonelli-Maruyoshi-Tanzini states'' [J. Math. Phys. 53, 033504 (2012)

    Science.gov (United States)

    Felińska, Ewa; Jaskólski, Zbigniew; Kosztołowicz, Michał

    2012-12-01

    A flow in the formulation and proof of Lemma 2.7 of E. Felińska, Z. Jaskólski, and M. M. Kosztołowicz, J. Math. Phys. 53, 033504 (2012) is fixed in Sec. I of this Erratum. This has no consequences for the rest of the paper. An essential error was made in Theorems 3.5, 3.6, and Corollary 3.7 of Sec. III of E. Felińska, Z. Jaskólski, and M. M. Kosztołowicz, J. Math. Phys. 53, 033504 (2012). As it was pointed out by V. Mazorchuk and K. Zhao ["Simple Virasoro modules which are locally finite over positive part," e-print arXiv:1205.5937v2 [math.RT

  14. Infauna monitoring Horns Rev offshore wind farm. Annual status report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Bech, M.; Leonhars, S.B.; Pedersen, John

    2004-05-15

    ELSAM and ELTRA have established an offshore wind farm with an output of 160 MW in the waters of Horns Rev 1420 km off Blaevands Huk, which is the most westerly point of Denmark. The first phase of construction of the wind farm started in spring 2002. Before the construction activities took place, a baseline description of the benthos was conducted as a part of an environmental monitoring programme for the establishment of the Horns Rev Offshore Wind Farm. The baseline surveys for the present monitoring programme were conducted in the wind farm area on three occasions: spring 1999, spring 2001 and September 2001. In designated reference areas, surveys were conducted in spring 1999 and September 2001. The reference areas in 1999 and September 2001 were placed at two different geographical locations because the survey in September 2001 was planned to be a part of a fish monitoring programme. A comparison between the baseline study in spring 2001 and the baseline study in autumn 2001 clearly revealed that the biomass of most species increased considerably from spring to September. Despite the increase in biomass, the overall distribution of the species and their relative abundance did not change. In order to use the baseline data to investigate a possible impact after the construction of the wind farm, it was essential to arrange the monitoring programme either in spring or in September 2003, because the baseline studies were conducted in these periods. The monitoring programme was conducted in September 2003 after the wind farm had become operational, parallel with the survey on hard bottom substrates. The impacts of the wind farm on the benthic fauna (infauna) in the area were mainly expected to be due to the alteration of the local currents. As the changes in the currents are only minor, impacts on the water chemistry and on the benthic fauna resulting from hydrodynamic causes were expected to be limited or non-existent. The main objective of the present monitoring

  15. Infauna monitoring Horns Rev offshore wind farm. Annual state report 2004

    Energy Technology Data Exchange (ETDEWEB)

    Bech, M.; Frederiksen, R.; Pedersen, John; Leonhard, S.B.

    2005-04-15

    A total of 40 species were identified from the surveys in the Horns Rev area in September 2004 while 42 species were identified in 2003 and 47 species in September 2001. The decline in the number of species occurred both inside the wind farm and reference areas, which indicates that the decline could be a combination of changes in sediment characteristics and natural variation rather than an effect from the establishment of the wind farm. More species were not associated with the hard substrate at the turbine sites in 2004 compared to 2003, while in 2001, more species were associated with fine-grained sand. The median sediment grain size increased from 2001 to 2003 to 2004, which suggests that the velocity of the current increased, but modelling calculations on current speed predicted a 2% reduction in the wind farm area and up to a 15% reduction very close to the scour protection. These results agreed with the grain sizes found at the stations 5, 25 and 100 metres from the scour protection. At most stations, the medium grain size was 5 metres lower from the scour protection compared with the station 100 metres from the scour protection, which indicates that the velocity of the current was lower close to the scour protection. No significant impact on the infauna in the wind farm area was detectable concerning distance-related effects. Though general reductions in the population size of some of the character species in the surveyed areas might be related to changes in the sediment structure, the infauna community at Horns Rev showed no obvious sign of stress response as a consequence of possible impact from construction and operating activities. New species were observed in 2003 and 2004 and some of these might be a result of sediment characteristics, less predation or natural variation. The recording of other species might be a result of the introduction of hard bottom habitants in the wind farm area. The density of the most abundant bivalves and bristle worms was

  16. Persistence of attenuated HIV-1 rev alleles in an epidemiologically linked cohort of long-term survivors infected with nef-deleted virus

    Directory of Open Access Journals (Sweden)

    Wesselingh Steven L

    2007-07-01

    Full Text Available Abstract Background The Sydney blood bank cohort (SBBC of long-term survivors consists of multiple individuals infected with nef-deleted, attenuated strains of human immunodeficiency virus type 1 (HIV-1. Although the cohort members have experienced differing clinical courses and now comprise slow progressors (SP as well as long-term nonprogressors (LTNP, longitudinal analysis of nef/long-terminal repeat (LTR sequences demonstrated convergent nef/LTR sequence evolution in SBBC SP and LTNP. Thus, the in vivo pathogenicity of attenuated HIV-1 strains harboured by SBBC members is dictated by factors other than nef/LTR. Therefore, to determine whether defects in other viral genes contribute to attenuation of these HIV-1 strains, we characterized dominant HIV-1 rev alleles that persisted in 4 SBBC subjects; C18, C64, C98 and D36. Results The ability of Rev derived from D36 and C64 to bind the Rev responsive element (RRE in RNA binding assays was reduced by approximately 90% compared to Rev derived from HIV-1NL4-3, C18 or C98. D36 Rev also had a 50–60% reduction in ability to express Rev-dependent reporter constructs in mammalian cells. In contrast, C64 Rev had only marginally decreased Rev function despite attenuated RRE binding. In D36 and C64, attenuated RRE binding was associated with rare amino acid changes at 3 highly conserved residues; Gln to Pro at position 74 immediately N-terminal to the Rev activation domain, and Val to Leu and Ser to Pro at positions 104 and 106 at the Rev C-terminus, respectively. In D36, reduced Rev function was mapped to an unusual 13 amino acid extension at the Rev C-terminus. Conclusion These findings provide new genetic and mechanistic insights important for Rev function, and suggest that Rev function, not Rev/RRE binding may be rate limiting for HIV-1 replication. In addition, attenuated rev alleles may contribute to viral attenuation and long-term survival of HIV-1 infection in a subset of SBBC members.

  17. Analysis of translesion DNA synthesis activity of the human REV1 protein, which is a key player in radiation-induced mutagenesis

    Energy Technology Data Exchange (ETDEWEB)

    Masuda, Yuji; Kamiya, Kenji [Hiroshima Univ., Research Institute for Radiation Biology and Medicine, Kasumi, Hiroshima (Japan)

    2003-07-01

    Ionizing radiation frequently causes oxidative DNA damage in cells. It has been suggested that functions of the REV1 gene are induction of mutations and prevention of cell death caused by ionizing radiation through the damage bypass DNA replication. The gene product possesses a deoxycytidyl transferase activity, which is required for translesion DNA synthesis of a variety of damaged bases and an abasic site. To elucidate molecular mechanisms of the mutagenesis and translesion DNA synthesis, it is important to characterize the enzymatic properties of the REV1 protein. Here, we describe a novel method for purifying the recombinant human REV1 protein and the anzymatic properties of the protein. We established an efficient system for induction of the recombinant human REV1 protein in Escherichia coli cells. The REV1 protein was purified to homogeneity using nickel-chelating sepharose, heparin sepharose and superdex 200 chromatography. When purified by this method, REV1 protein is free of endo-, exonuclease and DNA polymerase activities. The purified REV1 protein is suitable for enzymological studies, and we used this to biochemical characterization. The REV1 protein inserts dCMP opposite templates G, A, T, C and an abasic site and inserts dGMP and dTMP opposite template G. Kinetic analysis provided evidence for high efficiency for dCMP insertion opposite template G and an abasic site, suggesting that the REV1 protein play a role in translesion DNA synthesis of an abasic site. (author)

  18. Comment on "A study of phantom scalar field cosmology using Lie and Noether symmetries" [Int. J. Mod. Phys. D 25 (2016) 1650051

    CERN Document Server

    Paliathanasis, Andronikos; Tsamparlis, Michael

    2016-01-01

    We show that the recent results of \\ [Int. J. Mod. Phys. D 25 (2016) 1650051] on the application of Lie/Noether symmetries in scalar field cosmology are well-known in the literature while the problem could have been solved easily under a coordinate transformation. That follows from the property, that the admitted group of invariant transformations of dynamical system is independent on the coordinate system.

  19. Note: Derivation of two-photon circular dichroism - Addendum to "two-photon circular dichroism" [J. Chem. Phys. 62, 1006 (1975)

    OpenAIRE

    Friese, Daniel Henrik

    2015-01-01

    Published version, also available at http://dx.doi.org/10.1063/1.4930017 This addendum shows the detailed derivation of the fundamental equations for two-photon circular dichroism which are given in a very condensed form in the original publication [I. Tinoco, J. Chem. Phys. 62, 1006 (1975)]. In addition, some minor errors are corrected and some of the derivations in the original publication are commented.

  20. Comment on "Four-component relativistic theory for NMR parameters: Unified formulation and numerical assessment of different approaches" [J. Chem. Phys. 130, 144102 (2009)

    CERN Document Server

    Szmytkowski, Radoslaw

    2011-01-01

    In the paper commented on [J. Chem. Phys. 130 (2009) 144102], Cheng et al. derived a formula for the magnetic dipole shielding constant $\\sigma$ for the Dirac one-electron atom in its ground state. That formula involves an infinite series of ratios of the Euler's gamma functions. We show that with some algebra the series may be expressed in terms of elementary functions. This leads to a simple closed-form expression for the shielding constant.

  1. Comment on ``Monte Carlo investigation of current voltage and avalanche noise in GaN double-drift impact diodes'' [J. Appl. Phys. 97, 043709 (2005)

    Science.gov (United States)

    Dash, G. N.

    2005-11-01

    The avalanche noise behavior of impact avalanche transit-time (IMPATT) diodes has been modeled by Reklaitis and Reggiani [J. Appl. Phys. 97, 043709 (2005)]. They have obtained general agreement of their results with those of McIntyre [IEEE Trans. Electron Devices ED-13, 164 (1966)]. However, McIntyre's theory predicts the opposite noise behavior from that observed in the IMPATT diode. Hence the applicability of the noise model of Reklaitis and Reggiani to IMPATT diode is questionable.

  2. The calculational VVER burnup Credit Benchmark No.3 results with the ENDF/B-VI rev.5 (1999)

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Gual, Maritza [Centro de Tecnologia Nuclear, La Habana (Cuba). E-mail: mrgual@ctn.isctn.edu.cu

    2000-07-01

    The purpose of this papers to present the results of CB3 phase of the VVER calculational benchmark with the recent evaluated nuclear data library ENDF/B-VI Rev.5 (1999). This results are compared with the obtained from the other participants in the calculations (Czech Republic, Finland, Hungary, Slovaquia, Spain and the United Kingdom). The phase (CB3) of the VVER calculation benchmark is similar to the Phase II-A of the OECD/NEA/INSC BUC Working Group benchmark for PWR. The cases without burnup profile (BP) were performed with the WIMS/D-4 code. The rest of the cases have been carried with DOTIII discrete ordinates code. The neutron library used was the ENDF/B-VI rev. 5 (1999). The WIMS/D-4 (69 groups) is used to collapse cross sections from the ENDF/B-VI Rev. 5 (1999) to 36 groups working library for 2-D calculations. This work also comprises the results of CB1 (obtained with ENDF/B-VI rev. 5 (1999), too) and CB3 for cases with Burnup of 30 MWd/TU and cooling time of 1 and 5 years and for case with Burnup of 40 MWd/TU and cooling time of 1 year. (author)

  3. 77 FR 30546 - Notice of Proposed Information Collection: Comment Request; Form HUD-40221(rev) “LOCCS/VRS Self...

    Science.gov (United States)

    2012-05-23

    ...) ``LOCCS/VRS Self-Help Homeownership Opportunity Program Payment Voucher'' AGENCY: Office of the Assistant... Payment Voucher'' prior to requesting a drawdown of funds through LOCCS/VRS. Title of Proposal: Form HUD-40221(rev) ``LOCCS/VRS Self-help Homeownership Opportunity Program Payment Voucher''. OMB Control...

  4. Specific amino acid residues are involved in substrate discrimination and template binding of human REV1 protein.

    Science.gov (United States)

    Piao, Jinlian; Masuda, Yuji; Kamiya, Kenji

    2010-02-05

    REV1 is a member of the Y-family DNA polymerases, but is atypical in utilizing only dCTP with a preference for guanine (G) as the template. Crystallography of the REV1-DNA-dCTP ternary complex has revealed a unique mechanism by which template G is evicted from the DNA helix and incoming dCTP is recognized by an arginine residue in an alpha-loop, termed the N-digit. To better understand functions of its individual amino acid residues, we made a series of mutant human REV1 proteins. We found that R357 and L358 play vital roles in template binding. Furthermore, extensive mutation analysis revealed a novel function of R357 for substrate discrimination, in addition to previously proposed specific interaction with incoming dCTP. We found that the binding pocket for dCTP of REV1 has also significant but latent affinity for dGTP. The results suggest that the positive charge on R357 could prevent interaction with dGTP. We propose that both direct and indirect mechanisms mediated by R357 ensure specificity for dCTP.

  5. OPERATIONAL CIRCULAR NO2 (REV. 1) - APRIL 1998 'CONDITIONS OF ACCESS TO THE FENCED PARTS OF THE CERN SITE'

    CERN Multimedia

    Human Resources Division

    2001-01-01

    Subsidiary document 'Implementation Measures' (Rev. 2) - April 2001 The subsidiary document has been amended. Copies are available from Divisional Secretariats and at CERN card issue points. Note : Administrative and operational circulars, as well as the lists of those in force, are available for consultation on WWW : ADMINISTRATIVE CIRCULARS OPERATIONAL CIRCULARS

  6. Commentary of Hu Yihong' s Animation Theory%胡依红动画理论述评

    Institute of Scientific and Technical Information of China (English)

    屈立丰

    2012-01-01

    本文对上海美影厂女动画家胡依红的动画理论思想与动画创作实践做了简单梳理,细致分析了其“动画创新论”和“动画本体论”的理论来源和框架构成,对其动画理论思想和动画实践之间的互动关系进行了初步讨论。%This paper gave a brief introduction of the animation theory and creations by Hu Yihong, a female animator from Shanghai Animation Film Studio, made a detailed analysis of the sources and framework of her "ani- mation innovation theory" and "animation ontology", and finally presented a tentative discussion about the interac- tion between her ideology of animation theory and animation creation.

  7. A New Arthropod, Guangweicaris Luo, Fu et Hu gen. nov.from the Early Cambrian Guanshan Fauna, Kunming, China

    Institute of Scientific and Technical Information of China (English)

    LUO Huilin; FU Xiaoping; HU Shixue; LI Yong; HOU Shuguang; YOU Ting; PANG Jiyuan; LIU Qi

    2007-01-01

    The Guanshan Fauna is a soft-bodied fauna dominated by arthropods (including trilobites,trilobitoides, Tuzoia, Isoxys, and bradorids) in association with priapulids, brachiopods,anomalocaridids, vetulicoliids, sponges, chancellorids, and echinoderms. This paper reports and describes a new arthropod from the yellowish green mudstone at the lower part of the Wulongqing Formation, Canglangpuan Stage, Lower Cambrian in Kunming, Yunnan, China. The stratigraphic and geographic distribution, classification, fossil preservation, life style of this new arthropod and comparisons with other fossil arthropods are also discussed in details. The discovery and research of the non-mineralized arthropod, Guangweicaris Luo, Fu et Hu gen. nov. from the Guanshan Fauna adds new members to the taxonomic list and provides new information to the evolution of early arthropods.Furthermore, this study would shed new light into the "Cambrian Explosion" and the evolution of early life.

  8. Chinese President Hu Jintao and Austrian President Heinz Fischer Send Messages of Congratulations on 2006 China Year in Austria

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    <正>Editor’s note: In January 2004, Benita Ferrero-Waldner, Austrian foreign minister at the time, announced that China Year would be held in Austria in 2006 to mark the 35th anniversary of the establishment of diplomatic relations between Austria and China and to promote understanding of China and bilateral cooperation. The two sides decided after consultation that the activities for the China Year would be jointly organized by the Austria-China Friendship Association and the Chinese People’s Association for Friendship with Foreign Countries. The governments of the two countries attach great importance to the holding of the China Year. Chinese President Hu Jintao, Austrian President Heinz Fischer and Federal Chancellor Wolfgang Schussel and other state leaders

  9. Effects of vesicular-arbuscular mycorrhizae on the drought resistance of wild jujube (Zizyphs spinosus Hu) seedlings

    Institute of Scientific and Technical Information of China (English)

    LU Jinying; LIU Min; MAO Yongmin; SHEN Lianying

    2007-01-01

    The current study explored the effects of vesicular-arbuscular mycorrhizae (VAM) inoculation on the growth and water requirement of pot-grown wild jujube (Zizyphs spinosus Hu).Three water regimes (20%,40% and 60% of soil water content) were conducted.The VAM inoculation could significantly increase plant growth (including plant height,leaf area,and fresh and dry mass),enhance relative leaf water content,photosynthetic rates,transpiration rates and stomatal conductance,and improve plant drought tolerance.The water consumption of the mycorrhizal plants producing 1 g of dry matter was 18.7%-26.6% lower than the consumption of non-mycorrhizal plants grown under the same soil water content conditions.

  10. Antioxidant status in pregnant ewes vaccinated with Rev 1 against brucellosis

    Directory of Open Access Journals (Sweden)

    W. S. Al- Khafaji

    2012-01-01

    Full Text Available The aim of this study was to examine the changes in the indicators of free radicals and antioxidant activity, represented by malondialdehyde and glutathione peroxidase in the sera of ewes vaccinated with Rev 1 vaccine. The experiment included 28 animals which were divided into four equal groups. Animals of the first and second groups were vaccinated subcutaneously with 2×109 and 2×107 colony forming units (CFU, respectively, whereas the animals of third and fourth groups were vaccinated conjunctively with 2×109 and 2×107 CFU, respectively. Sera were collected monthly for 6 months. Antibody responses were assessed by classical tests (Rose Bengal test, tube agglutination and 2-mercaptoethanol tests in comparison with competitive ELISA. The antibody titers were higher and remained for along period in the subcutaneously vaccinated groups with the two doses compared those vaccinated conjunctively. There was a significant increase in serum glutathione peroxidase activity in the 8th week post vaccination in subcutaneously vaccinated groups and during the 12th week in those vaccinated conjunctively. Significant increase of serum malondialdehyde levels occurred during the 4th week in those vaccinated conjunctively and in 8th week in those vaccinated subcutaneously. This study concluded that the route of administration of the vaccine affects glutathione peroxidase activity and malondialdehyde level, which act as indicators of oxidative stress response, more than the vaccine dose.

  11. Effect of nacelle on the wake meandering in Horns Rev wind farm

    Science.gov (United States)

    Yang, Xiaolei; Foti, Daniel; Sotiropoulos, Fotis

    2016-11-01

    Turbine wake meandering has considerable effects on the velocity deficit and turbulence intensity in the wake. However, the mechanism for wake meandering is still not well understood and low-order models cannot take into account the wake meandering effects accurately. A recent work by Kang, Yang and Sotiropoulos showed that the nacelle has a significant effect on the wake meandering of a hydrokinetic turbine. To examine the nacelle contributions to wake meandering and wake interactions in utility-scale wind farms, we simulate the atmospheric turbulent flow over the Horns Rev wind farm using large-eddy simulation with actuator type models. In a preliminary simulation on a coarse grid using actuator line model for turbine blades without a nacelle model, the computed power shows overall good agreement with field measurements. Fine grid simulations using an actuator surface model for turbine blades with and without a nacelle model are being carried out. The corresponding results will be presented with analysis on wake meandering dynamics using the technique proposed by Horward et al. and Foti et al.. This work was supported by DOE (DE-AC04-94AL85000), Xcel Energy (Grant RD4-13) and Sandia National Laboratories. Computational resources were provided by National Renewable Energy Laboratory and University of Minnesota Supercomputing Institute.

  12. Modeling RNA-ligand interactions: the Rev-binding element RNA-aminoglycoside complex.

    Science.gov (United States)

    Leclerc, F; Cedergren, R

    1998-01-15

    An approach to the modeling of ligand-RNA complexes has been developed by combining three-dimensional structure-activity relationship (3D-SAR) computations with a docking protocol. The ability of 3D-SAR to predict bound conformations of flexible ligands was first assessed by attempting to reconstruct the known, bound conformations of phenyloxazolines complexed with human rhinovirus 14 (HRV14) RNA. Subsequently, the same 3D-SAR analysis was applied to the identification of bound conformations of aminoglycosides which associate with the Rev-binding element (RBE) RNA. Bound conformations were identified by parsing ligand conformational data sets with pharmacophores determined by the 3D-SAR analysis. These "bioactive" structures were docked to the receptor RNA, and optimization of the complex was undertaken by extensive searching of ligand conformational space coupled with molecular dynamics computations. The similarity between the bound conformations of the ligand from the 3D-SAR analysis and those found in the docking protocol suggests that this methodology is valid for the prediction of bound ligand conformations and the modeling of the structure of the ligand-RNA complexes.

  13. Bird numbers and distribution in the Horns Rev offshore wind farm area. Annual status report 2003

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    This report presents data from six aerial surveys of birds in the Horns Rev wind farm area in 2003. Including 16 surveys conducted before construction of the wind farm started and three during the construction phase, a total of 25 surveys have been performed in the area since August 1999. Up until August 2002 the study area was surveyed from 26 north-south oriented, parallel transect lines. After that time four short transects were added eastwards from the previously easternmost transect. From August 2002 slight adjustments to the transect lines in the wind farm area had to be made in order to avoid collision, as survey altitude was 76 m and wind turbines are 110 m to highest wing tip. The six surveys in 2003 were performed on 13 February, 16 March, 23 April, 5 September, 4 and 30 December. The operational phase of the wind farm commenced in 2002. Hence the six surveys from 2003 are all considered post-construction data sets. A preliminary evaluation of the potential impact of the wind turbines on bird distributions has been carried out by comparison of these data to those from the 16 pre-construction surveys. (au)

  14. REVS: a radar-based enhanced vision system for degraded visual environments

    Science.gov (United States)

    Brailovsky, Alexander; Bode, Justin; Cariani, Pete; Cross, Jack; Gleason, Josh; Khodos, Victor; Macias, Gary; Merrill, Rahn; Randall, Chuck; Rudy, Dean

    2014-06-01

    Sierra Nevada Corporation (SNC) has developed an enhanced vision system utilizing fast-scanning 94 GHz radar technology to provide three-dimensional measurements of an aircraft's forward external scene topography. This threedimensional data is rendered as terrain imagery, from the pilot's perspective, on a Head-Up Display (HUD). The image provides the requisite "enhanced vision" to continue a safe approach along the flight path below the Decision Height (DH) in Instrument Meteorological Conditions (IMC) that would otherwise be cause for a missed approach. Terrain imagery is optionally fused with digital elevation model (DEM) data of terrain outside the radar field of view, giving the pilot additional situational awareness. Flight tests conducted in 2013 show that REVS™ has sufficient resolution and sensitivity performance to allow identification of requisite visual references well above decision height in dense fog. This paper provides an overview of the Enhanced Flight Vision System (EFVS) concept, of the technology underlying REVS, and a detailed discussion of the flight test results.

  15. Domain- and nucleotide-specific Rev response element regulation of feline immunodeficiency virus production.

    Science.gov (United States)

    Na, Hong; Huisman, Willem; Ellestad, Kristofor K; Phillips, Tom R; Power, Christopher

    2010-09-01

    Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analyses were performed in both an infectious FIV molecular clone and a FIV CAT-RRE reporter system. These studies disclosed that the stems within SLA (SA1, 2, 3, 4, and 5) of the RRE were critical but SA6 was not essential for FIV replication and CAT expression. These studies also revealed that the secondary structure rather than an antisense protein (ASP) mediates virus expression and replication in vitro. In addition, a single synonymous mutation within the FIV-RRE, SA3/45, reduced viral reverse transcriptase activity and p24 expression after transfection but in addition also showed a marked reduction in viral expression and production following infection.

  16. A computational screen for site selective A-to-I editing detects novel sites in neuron specific Hu proteins

    Directory of Open Access Journals (Sweden)

    Furey Terrence S

    2010-01-01

    Full Text Available Abstract Background Several bioinformatic approaches have previously been used to find novel sites of ADAR mediated A-to-I RNA editing in human. These studies have discovered thousands of genes that are hyper-edited in their non-coding intronic regions, especially in alu retrotransposable elements, but very few substrates that are site-selectively edited in coding regions. Known RNA edited substrates suggest, however, that site selective A-to-I editing is particularly important for normal brain development in mammals. Results We have compiled a screen that enables the identification of new sites of site-selective editing, primarily in coding sequences. To avoid hyper-edited repeat regions, we applied our screen to the alu-free mouse genome. Focusing on the mouse also facilitated better experimental verification. To identify candidate sites of RNA editing, we first performed an explorative screen based on RNA structure and genomic sequence conservation. We further evaluated the results of the explorative screen by determining which transcripts were enriched for A-G mismatches between the genomic template and the expressed sequence since the editing product, inosine (I, is read as guanosine (G by the translational machinery. For expressed sequences, we only considered coding regions to focus entirely on re-coding events. Lastly, we refined the results from the explorative screen using a novel scoring scheme based on characteristics for known A-to-I edited sites. The extent of editing in the final candidate genes was verified using total RNA from mouse brain and 454 sequencing. Conclusions Using this method, we identified and confirmed efficient editing at one site in the Gabra3 gene. Editing was also verified at several other novel sites within candidates predicted to be edited. Five of these sites are situated in genes coding for the neuron-specific RNA binding proteins HuB and HuD.

  17. Validation of the SCID-hu Thy/Liv mouse model with four classes of licensed antiretrovirals.

    Directory of Open Access Journals (Sweden)

    Cheryl A Stoddart

    Full Text Available BACKGROUND: The SCID-hu Thy/Liv mouse model of HIV-1 infection is a useful platform for the preclinical evaluation of antiviral efficacy in vivo. We performed this study to validate the model with representatives of all four classes of licensed antiretrovirals. METHODOLOGY/PRINCIPAL FINDINGS: Endpoint analyses for quantification of Thy/Liv implant viral load included ELISA for cell-associated p24, branched DNA assay for HIV-1 RNA, and detection of infected thymocytes by intracellular staining for Gag-p24. Antiviral protection from HIV-1-mediated thymocyte depletion was assessed by multicolor flow cytometric analysis of thymocyte subpopulations based on surface expression of CD3, CD4, and CD8. These mice can be productively infected with molecular clones of HIV-1 (e.g., the X4 clone NL4-3 as well as with primary R5 and R5X4 isolates. To determine whether results in this model are concordant with those found in humans, we performed direct comparisons of two drugs in the same class, each of which has known potency and dosing levels in humans. Here we show that second-generation antiretrovirals were, as expected, more potent than their first-generation predecessors: emtricitabine was more potent than lamivudine, efavirenz was more potent than nevirapine, and atazanavir was more potent than indinavir. After interspecies pharmacodynamic scaling, the dose ranges found to inhibit viral replication in the SCID-hu Thy/Liv mouse were similar to those used in humans. Moreover, HIV-1 replication in these mice was genetically stable; treatment of the mice with lamivudine did not result in the M184V substitution in reverse transcriptase, and the multidrug-resistant NY index case HIV-1 retained its drug-resistance substitutions. CONCLUSION: Given the fidelity of such comparisons, we conclude that this highly reproducible mouse model is likely to predict clinical antiviral efficacy in humans.

  18. Dieta de roedores sigmodontinos (Cricetidae en los bosques montanos tropicales de Huánuco, Perú

    Directory of Open Access Journals (Sweden)

    Maggie C. Noblecilla

    2013-06-01

    Full Text Available Se analizó el contenido estomacal de cinco especies de roedores sigmodontinos: Akodon orophilus, Microry-zomys altissimus, M. minutus, Thomasomys notatus yT. kalinowskii, procedentes de los bosques montanos de Huánuco, Perú (2564 - 3850 m de altitud. Concluimos que A. orophiluses insectívora por haber presentado un alto volumen de artrópodos (adultos y larvas en el contenido estomacal (90,1%; mientras que T. notatusy T. kalinowskiison principalmente herbívoras por el alto volumen de materia vegetal, 89% y 67,75% respectiva-mente; y que M. altissimusy M. minutusson omnívoras por presentar volúmenes similares tanto para vegetales como para artrópodos. Thomasomys kalinowskiies considerada generalista por la mayor amplitud de nicho (4,61, mientras A. orophiluses considerada especialista por el menor valor (1,70. Akodon orophilusmostró una preferencia por el consumo de artrópodos adultos al tener un bajo coeficiente de variación (CV= 20% y también un significativo aumento en el consumo de larvas de artrópodos en la época húmeda, siendo la única especie con variación estacional en la dieta. Por otro lado, la sobreposición de nicho fue menor a 0,75 en el 80% de los pares de especies comparados, indicando una baja similitud en la dieta. La mayor similitud en la dieta se observó entre M. altissimus- T. notatus(0,822 y M. minutus - T. kalinowskii(0,816. Se concluye que estos roedores sigmodontinos, simpátricos en los bosques montanos de Huánuco, exhiben dietas disímiles, probablemente como una estrategia para evitar o aminorar la competencia interespecífica.

  19. Validation of the SCID-hu Thy/Liv mouse model with four classes of licensed antiretrovirals.

    Science.gov (United States)

    Stoddart, Cheryl A; Bales, Cheryl A; Bare, Jennifer C; Chkhenkeli, George; Galkina, Sofiya A; Kinkade, April N; Moreno, Mary E; Rivera, José M; Ronquillo, Rollie E; Sloan, Barbara; Black, Paul L

    2007-08-01

    The SCID-hu Thy/Liv mouse model of HIV-1 infection is a useful platform for the preclinical evaluation of antiviral efficacy in vivo. We performed this study to validate the model with representatives of all four classes of licensed antiretrovirals. Endpoint analyses for quantification of Thy/Liv implant viral load included ELISA for cell-associated p24, branched DNA assay for HIV-1 RNA, and detection of infected thymocytes by intracellular staining for Gag-p24. Antiviral protection from HIV-1-mediated thymocyte depletion was assessed by multicolor flow cytometric analysis of thymocyte subpopulations based on surface expression of CD3, CD4, and CD8. These mice can be productively infected with molecular clones of HIV-1 (e.g., the X4 clone NL4-3) as well as with primary R5 and R5X4 isolates. To determine whether results in this model are concordant with those found in humans, we performed direct comparisons of two drugs in the same class, each of which has known potency and dosing levels in humans. Here we show that second-generation antiretrovirals were, as expected, more potent than their first-generation predecessors: emtricitabine was more potent than lamivudine, efavirenz was more potent than nevirapine, and atazanavir was more potent than indinavir. After interspecies pharmacodynamic scaling, the dose ranges found to inhibit viral replication in the SCID-hu Thy/Liv mouse were similar to those used in humans. Moreover, HIV-1 replication in these mice was genetically stable; treatment of the mice with lamivudine did not result in the M184V substitution in reverse transcriptase, and the multidrug-resistant NY index case HIV-1 retained its drug-resistance substitutions. Given the fidelity of such comparisons, we conclude that this highly reproducible mouse model is likely to predict clinical antiviral efficacy in humans.

  20. Endogenous rhythmicity of Bmal1 and Rev-erb alpha in the hamster pineal gland is not driven by norepinephrine.

    Science.gov (United States)

    Wongchitrat, Prapimpun; Felder-Schmittbuhl, Marie-Paule; Phansuwan-Pujito, Pansiri; Pévet, Paul; Simonneaux, Valérie

    2009-05-01

    Pineal melatonin is synthesized with daily and seasonal rhythms following the hypothalamic clock-driven release of norepinephrine (NE). The pineal gland of rats and mice, like the biological clock, expresses a number of clock genes. However, the role of pineal clock elements in pineal physiology is still unknown. We examined the expression and regulation of several clock genes (Per1, Cry2, Bmal1 and Rev-erb alpha) under different lighting conditions or following adrenergic treatments in the Syrian hamster, a seasonal rodent. We found that Per1 and Cry2 genes were similarly regulated by the nocturnal release of NE: levels of Per1 and Cry2 mRNA displayed a nocturnal increase that was maintained after 2 days in constant darkness (DD) but abolished after 2 days under constant light (LL), a condition that suppresses endogenous NE release, or after an early night administration of the adrenergic antagonist propranolol. In contrast, Bmal1 and Rev-erb alpha exhibited a different pattern of expression and regulation. mRNA levels of both clock genes displayed a marked daily variation, maintained in DD, with higher values at midday for Bmal1 and at day/night transition for Rev-erb alpha. Remarkably, the daily variation of both Bmal1 and Rev-erb alpha mRNA was maintained in LL conditions and was not affected by propranolol. This study confirms the daily regulation of Per1 and Cry2 gene expression by NE in the pineal gland of rodents and shows for the first time that a second set of clock genes, Bmal1 and Rev-erb alpha are expressed with a circadian rhythm independent of the hypothalamic clock-driven noradrenergic signal.

  1. Infección por Gnathostoma (Spirurida: Gnathostomatidae en Hoplias microlepis: prevalencia, correlación con la talla del pez, huéspedes e implicaciones para salud pública en Ecuador

    Directory of Open Access Journals (Sweden)

    Juan José Alava

    2009-12-01

    Conclusiones. La gnathostomiasis sigue siendo prevalente en la zona costera de Ecuador y el agente patógeno es aún encontrado en el huésped intermediario. Diversos mamíferos neotropicales estarían actuando como huésped reservorio definitivo en el ciclo biológico de Gnathostoma en Ecuador.

  2. HuCNS-SC Human NSCs Fail to Differentiate, Form Ectopic Clusters, and Provide No Cognitive Benefits in a Transgenic Model of Alzheimer's Disease

    Directory of Open Access Journals (Sweden)

    Samuel E. Marsh

    2017-02-01

    Full Text Available Transplantation of neural stem cells (NSCs can improve cognition in animal models of Alzheimer's disease (AD. However, AD is a protracted disorder, and prior studies have examined only short-term effects. We therefore used an immune-deficient model of AD (Rag-5xfAD mice to examine long-term transplantation of human NSCs (StemCells Inc.; HuCNS-SCs. Five months after transplantation, HuCNS-SCs had engrafted and migrated throughout the hippocampus and exhibited no differences in survival or migration in response to β-amyloid pathology. Despite robust engraftment, HuCNS-SCs failed to terminally differentiate and over a quarter of the animals exhibited ectopic human cell clusters within the lateral ventricle. Unlike prior short-term experiments with research-grade HuCNS-SCs, we also found no evidence of improved cognition, no changes in brain-derived neurotrophic factor, and no increase in synaptic density. These data, while disappointing, reinforce the notion that individual human NSC lines need to be carefully assessed for efficacy and safety in appropriate long-term models.

  3. Short communication. Employment of molecular markers to develop tetraploid “supermale” asparagus from andromonoecious plants of the landrace ‘Morado de Huétor’

    Directory of Open Access Journals (Sweden)

    Jose J. Regalado

    2014-10-01

    Full Text Available The aim of this work was the development of new “supermale” genotypes retaining the highest genetic diversity possible of the tetraploid asparagus landrace ‘Morado de Huétor’. The elite andromonoecious male HT664 of the ‘Morado de Huétor’ asparagus landrace and the andromonoecious hybrid male HC027, obtained by crossing between this landrace and a commercial cultivar of Asparagus officinalis, were selected for self-pollination to produce possible “supermales” with genes of ‘Morado de Huétor’ (SMHT. To confirm the hybrid nature of HC027, we characterized this genotype with EST-SSR (Expressed Sequence Tag-derived Simple Sequence Repeats markers. We also adopted the sex-linked marker Asp1-T7 for sex determination in ‘Morado de Huétor’ and the resulting hybrids between this landrace and other commercial cultivars. Asp1-T7 marker was used for the selection and genotyping of SMHT. “All-male” cultivars with a different genetic background can be generated by crossing females with these new “supermale” genotypes, and the agronomical traits of these new cultivars would be very different from the “all-male” cultivars currently available in the market, making them extremely interesting for asparagus breeding programs.

  4. On the Theoretical Character of Hu Jintao's Youth Viewpoint%胡锦涛同志青年观的理论品格思想研究

    Institute of Scientific and Technical Information of China (English)

    邹洪伟

    2009-01-01

    Mr. Hu Jintao has delivered lots pithy opinions on the youth work and the healthy growth of Chi-nese youths, with which Mr. Hu has developed the Marxist youth theory to its new level. This paper makes a brief illustration of the guidelines, the kernel, the goal and the historical mission of the youth work and the healthy growth of Chinese youths mentioned by Mr. Hu, trying to discuss the theoretical character of Mr. Hu Jintao's youth viewpoint.%胡锦涛同志对中国青年成长成才和青年工作提出了诸多精辟的观点,开拓了马克思主义青年观的新境界.本文就其中青年成长成才和青年工作的指导思想、核心、努力目标和历史使命等问题进行了简要阐述,充分展现胡锦涛同志青年观的理论品格.

  5. Purchase of Microwave Reactors for Implementation of Small-scale Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate Curriculum and Synthetic Chemistry Research at HU

    Science.gov (United States)

    2015-05-16

    Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate Curriculum and Synthetic Chemistry Research at HU The views, opinions and/or...Research Triangle Park, NC 27709-2211 Microwave laboratory facility, Undergraduate organic chemistry laboratory, Implementation/Development of...Purchase of Microwave Reactors for Implementation of Small-scale Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate

  6. HuD promotes BDNF expression in brain neurons via selective stabilization of the BDNF long 3'UTR mRNA.

    Directory of Open Access Journals (Sweden)

    Megan Allen

    Full Text Available Complex regulation of brain-derived neurotrophic factor (BDNF governs its intricate functions in brain development and neuronal plasticity. Besides tight transcriptional control from multiple distinct promoters, alternative 3'end processing of the BDNF transcripts generates either a long or a short 3'untranslated region (3'UTR. Previous reports indicate that distinct RNA sequence in the BDNF 3'UTRs differentially regulates BDNF production in the brain to accommodate neuronal activity changes, conceivably through differential interactions with undefined trans-acting factors that regulate stability and translation of these BDNF mRNA isoforms. In this study, we report that the neuronal RNA-binding protein (RBP HuD interacts with a highly conserved AU-rich element (ARE specifically located in the BDNF long 3'UTR. Such interaction is necessary and sufficient for selective stabilization of mRNAs that contain the BDNF long 3'UTR in vitro and in vivo. Moreover, in a HuD transgenic mouse model, the BDNF long 3'UTR mRNA is increased in the hippocampal dentate granule cells (DGCs, leading to elevated expression of BDNF protein that is transported and stored in the mossy fiber (MF terminals. Our results identify HuD as the first trans-acting factor that enhances BDNF expression specifically through the long 3'UTR and a novel mechanism that regulates BDNF protein production in selected neuronal populations by HuD abundance.

  7. The problem of the ethical root of truth (A tribute to Antonio Rodríguez Huéscar on his birth centenary

    Directory of Open Access Journals (Sweden)

    Juan Padilla

    2013-12-01

    Full Text Available This paper deals with the meaning of truth as «coincidence of man with himself» accordingto Ortega y Gasset and his disciple rodríguez huéscar. What the concept does not mean and which implications it has are also explained.

  8. PARP1 promotes gene expression at the post-transcriptional level by modulating the RNA-binding protein HuR

    Science.gov (United States)

    Ke, Yueshuang; Han, Yanlong; Guo, Xiaolan; Wen, Jitao; Wang, Ke; Jiang, Xue; Tian, Xue; Ba, Xueqing; Boldogh, Istvan; Zeng, Xianlu

    2017-01-01

    Poly(ADP-ribosyl)ation (PARylation) is mainly catalysed by poly-ADP-ribose polymerase 1 (PARP1), whose role in gene transcription modulation has been well established. Here we show that, in response to LPS exposure, PARP1 interacts with the adenylateuridylate-rich element-binding protein embryonic lethal abnormal vision-like 1 (Elavl1)/human antigen R (HuR), resulting in its PARylation, primarily at site D226. PARP inhibition and the D226 mutation impair HuR's PARylation, nucleocytoplasmic shuttling and mRNA binding. Increases in mRNA level or stability of pro-inflammatory cytokines/chemokines are abolished by PARP1 ablation or inhibition, or blocked in D226A HuR-expressing cells. The present study demonstrates a mechanism to regulate gene expression at the post-transcriptional level, and suggests that blocking the interaction of PARP1 with HuR could be a strategy to treat inflammation-related diseases that involve increased mRNA stability. PMID:28272405

  9. Priming with r-metHuSCF and filgrastim or chemotherapy and filgrastim in patients with malignant lymphomas: a randomized phase II pilot study of mobilization and engraftment

    DEFF Research Database (Denmark)

    Johnsen, Hanne; Geisler, C; Juvonen, E

    2011-01-01

    SCF has been shown to synergize with G-CSF to mobilize CD34(+) PBPCs. In this study we report results from this combination after a phase II trial of 32 patients with malignant lymphoma randomized to receive recombinant methionyl human SCF (ancestim, r-metHuSCF) in combination with recombinant me...

  10. Investigation of the HU-density conversion method and comparison of dose distribution for dose calculation on MV cone beam CT images

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Min Joo; Lee, Seu Ran; Suh, Tae Suk [Dept. of Biomedical Engineering, The Catholic University of Korea, Bucheon (Korea, Republic of)

    2011-11-15

    Modern radiation therapy techniques, such as Image-guided radiation therapy (IGRT), Adaptive radiation therapy (ART) has become a routine clinical practice on linear accelerators for the increase the tumor dose conformity and improvement of normal tissue sparing at the same time. For these highly developed techniques, megavoltage cone beam computed tomography (MVCBCT) system produce volumetric images at just one rotation of the x-ray beam source and detector on the bottom of conventional linear accelerator for real-time application of patient condition into treatment planning. MV CBCT image scan be directly registered to a reference CT data set which is usually kilo-voltage fan-beam computed tomography (kVFBCT) on treatment planning system and the registered image scan be used to adjust patient set-up error. However, to use MV CBCT images in radiotherapy, reliable electron density (ED) distribution are required. Patients scattering, beam hardening and softening effect caused by different energy application between kVCT, MV CBCT can cause cupping artifacts in MV CBCT images and distortion of Houns field Unit (HU) to ED conversion. The goal of this study, for reliable application of MV CBCT images into dose calculation, MV CBCT images was modified to correct distortion of HU to ED using the relationship of HU and ED from kV FBCT and MV CBCT images. The HU-density conversion was performed on MV CBCT image set using Dose difference map was showing in Figure 1. Finally, percentage differences above 3% were reduced depending on applying density calibration method. As a result, total error co uld be reduced to under 3%. The present study demonstrates that dose calculation accuracy using MV CBCT image set can be improved my applying HU-density conversion method. The dose calculation and comparison of dose distribution from MV CBCT image set with/without HU-density conversion method was performed. An advantage of this study compared to other approaches is that HU

  11. Horns Rev offshore wind power farm. Environmental impact assessment on water quality

    Energy Technology Data Exchange (ETDEWEB)

    Andersen, Per

    2000-05-15

    As part of an overall Environmental Impact Assessment (EIA) undertaken in connection with a planned 150 MW offshore wind farm at Horns Rev, an assessment was made of the effects the wind farm would have on the water quality in the area. This EIA study was drawn up in accordance with the guidelines laid down by the Danish Ministry of Environment and Energy in the publication 'Guidelines for the preparation of EIA studies for offshore wind farms'. Horns Rev is situated off Blaevands Huk, which is Denmark's most westerly point. It is a shallow reef with water depths between 2 and 9 metres and is primarily composed of sand, gravel and pebbles. Only local and minor changes are anticipated in connection with the currents, sediments and wave conditions during the production phase. These will occur in the immediate vicinity of the individual foundations. For these reasons, no changes are expected in the water quality. This also includes also the pelagic primary production and the occurrence of plankton in the area. Increased local copper contamination of phytoplankton and zooplankton may be expected during the production phase, as a result of the total annual discharge of 206 kg copper from the slip-rings in the wind turbines. The contamination will potentially result in a local reduction of the pelagic primary production and changes in the species composition of the plankton. The wind turbines will be sandblasted and painted once during their lifetime, as part of the routine maintenance. The sandblasting and painting will lead to a temporary spill of paint, paint waste and sand. The impacts on water quality and plankton production are unknown. It is recommended that factors such as the toxicity of the paint be investigated, and that spills and the impact of waste be reduced as much as possible. The water quality and the plankton in the wind farm area and along the cable line's passage to shore through the international protected area, will only be

  12. HRensembleHR. High resolution ensemble for Horns Rev. Final project report; Offshore wind power

    Energy Technology Data Exchange (ETDEWEB)

    2010-03-15

    The wind farm output of an offshore-farm such as Horns Rev changes between nearly constant output to highly variable power output. A balance responsible will therefore benefit from knowing the variability of a wind farm in advance. Some understanding of the observed variability and the corresponding forecast error on offshore wind farms had been gathered in the past few years, while a large fraction (about 60%) of the error still lacked understanding and required further intense research. This was the outset at the beginning of the HREnsemble project. Results from the wave study, variability study, ocean coupling, findings from the sensitivity experiments, the iEnKF short-term forecast and finally the demonstration phase have given significant synergy. The most basic research result in the project is that the two empirical mode decomposition approaches, Hilbert-Huang and later the Ensemble Empirical Mode Decomposition (EEMD). Both approaches confirmed that significant variability exists in offshore conditions. It was found that the variability of the short time-scales (24 minutes) are either not explicitly visible in the grid-scale of the NWP models or in the best case significantly smoothed out in all of the tested model configurations. If we interpret 2/3 of the variability due to vertical waves not present in the mean flow and 1/3 of the variability due to meso-scale weather, then the model results and EEMD are consistent. We can however not verify this theory. At present we do not know if EEMD counts neither the events correct nor whether the ensemble forecast suppresses variability. The existence of variability above the time-scale related to friction between ocean waves and air can in fact explain some of the inconsistent results published in the literature and set a question mark behind the correctness of the calculation of friction in wave, ocean and weather modelling. (LN)

  13. Mapping Wind Farm Loads and Power Production - A Case Study on Horns Rev 1

    Science.gov (United States)

    Galinos, Christos; Dimitrov, Nikolay; Larsen, Torben J.; Natarajan, Anand; Hansen, Kurt S.

    2016-09-01

    This paper describes the development of a wind turbine (WT) component lifetime fatigue load variation map within an offshore wind farm. A case study on the offshore wind farm Horns Rev I is conducted with this purpose, by quantifying wake effects using the Dynamic Wake Meandering (DWM) method, which has previously been validated based on CFD, Lidar and full scale load measurements. Fully coupled aeroelastic load simulations using turbulent wind conditions are conducted for all wind directions and mean wind speeds between cut-in and cut-out using site specific turbulence level measurements. Based on the mean wind speed and direction distribution, the representative 20-year lifetime fatigue loads are calculated. It is found that the heaviest loaded WT is not the same when looking at blade root, tower top or tower base components. The blade loads are mainly dominated by the wake situations above rated wind speed and the highest loaded blades are in the easternmost row as the dominating wind direction is from West. Regarding the tower components, the highest loaded WTs are also located towards the eastern central location. The turbines with highest power production are, not surprisingly, the ones facing a free sector towards west and south. The power production results of few turbines are compared with SCADA data. The results of this paper are expected to have significance for operation and maintenance planning, where the schedules for inspection and service activities can be adjusted to the requirements arising from the varying fatigue levels. Furthermore, the results can be used in the context of remaining fatigue lifetime assessment and planning of decommissioning.

  14. Detrimental ELAVL-1/HuR-dependent GSK3β mRNA stabilization impairs resolution in acute respiratory distress syndrome

    Science.gov (United States)

    Hoffman, Olivia; Burns, Nana; Vadász, István; Eltzschig, Holger K.; Edwards, Michael G.

    2017-01-01

    A hallmark of acute respiratory distress syndrome (ARDS) is accumulation of protein-rich edema in the distal airspaces and its removal is critical for patient survival. Previous studies have shown a detrimental role of Glycogen Synthase Kinase (GSK) 3β during ARDS via inhibition of alveolar epithelial protein transport. We hypothesized that post-transcriptional regulation of GSK3β could play a functional role in ARDS resolution. To address this hypothesis, we performed an in silico analysis to identify regulatory genes whose expression correlation to GSK3β messenger RNA utilizing two lung cancer cell line array datasets. Among potential regulatory partners of GSK3β, these studies identified the RNA-binding protein ELAVL-1/HuR (Embryonic Lethal, Abnormal Vision, Drosophila-Like) as a central component in a likely GSK3β signaling network. ELAVL-1/HuR is a RNA-binding protein that selectively binds to AU-rich elements of mRNA and enhances its stability thereby increasing target gene expression. Subsequent studies with siRNA suppression of ELAVL-1/HuR demonstrated deceased GSK3β mRNA and protein expression and improved clearance of FITC-albumin in A549 cells. Conversely, stabilization of ELAVL-1/HuR with the proteasome inhibitor MG-132 resulted in induction of GSK3β at mRNA and protein level and attenuated FITC-albumin clearance. Utilizing ventilator-induced lung injury or intra-tracheal installation of hydrochloric acid to induce ARDS in mice, we observed increased mRNA and protein expression of ELAVL-1/HuR and GSK3β. Together, our findings indicate a previously unknown interaction between GSK3β and ELAV-1 during ARDS, and suggest the inhibition of the ELAV-1- GSK3β pathways as a novel ARDS treatment approach. PMID:28196122

  15. The Anti-Inflammatory Effect and Intestinal Barrier Protection of HU210 Differentially Depend on TLR4 Signaling in Dextran Sulfate Sodium-Induced Murine Colitis.

    Science.gov (United States)

    Lin, Sisi; Li, Yongyu; Shen, Li; Zhang, Ruiqin; Yang, Lizhi; Li, Min; Li, Kun; Fichna, Jakub

    2017-02-01

    Ulcerative colitis (UC) is strongly associated with inflammation and intestinal barrier disorder. The nonselective cannabinoid receptor agonist HU210 has been shown to ameliorate inflamed colon in colitis, but its effects on intestinal barrier function and extraintestinal inflammation are unclear. To investigate the effects and the underlying mechanism of HU210 action on the UC in relation to a role of TLR4 and MAP kinase signaling. Wild-type (WT) and TLR4 knockout (Tlr4 (-/-)) mice were exposed to 4% dextran sulfate sodium (DSS) for 7 days. The effects of HU210 on inflammation and intestinal barrier were explored. Upon DSS challenge, mice suffered from bloody stool, colon shortening, intestinal mucosa edema, pro-inflammatory cytokine increase and intestinal barrier destruction with goblet cell depletion, increased intestinal microflora accompanied with elevated plasma lipopolysaccharide, reduced mRNA expression of the intestinal tight junction proteins, and abnormal ratio of CD4(+)/CD8(+) T cells in the intestinal Peyer's patches. Pro-inflammatory cytokines in the plasma and the lung, as well as pulmonary myeloperoxidase activity, indicators of extraintestinal inflammation were increased. Protein expression of p38α and pp38 was up-regulated in the colon of WT mice. Tlr4 (-/-) mice showed milder colitis. HU210 reversed the intestinal barrier changes in both strains of mice, but alleviated inflammation only in WT mice. Our study indicates that in experimental colitis, HU210 displays a protective effect on the intestinal barrier function independently of the TLR4 signaling pathway; however, in the extraintestinal tissues, the anti-inflammatory action seems through affecting TLR4-mediated p38 mitogen-activated protein kinase pathway.

  16. Cytoplasmic expression of HuR may be a valuable diagnostic tool for determining the potential for malignant transformation of oral verrucous borderline lesions

    Science.gov (United States)

    HABIBA, UMMA; KITAMURA, TETSUYA; YANAGAWA-MATSUDA, AYA; HIDA, KYOKO; HIGASHINO, FUMIHIRO; OHIRO, YOICHI; TOTSUKA, YASUNORI; SHINDOH, MASANOBU

    2014-01-01

    Oral verrucous carcinoma (OVC) is a low grade variant of oral squamous cell carcinoma, and oral verrucous hyperplasia (OVH) is a benign lesion without malignant features. However, pathologists are sometimes presented with borderline lesions and are indecisive as to diagnose them as benign or malignant. Thus, these lesions are tentatively termed oral verrucous lesions (OVLs). HuR is an ARE mRNA-binding protein, normally localized in the nucleus but cytoplasmic exportation is frequently observed in cancer cells. The present study aimed to elucidate whether expression of the HuR protein facilitates the diagnosis of true malignant lesions. Clinicopathological features were evaluated, and immunohistochemical analysis for p53, Ki67 and HuR proteins was performed in 48 cases of OVH, OVC and OVL, and the outcomes were correlated using appropriate statistical analysis. The association of these three proteins in relation to malignant transformation was analyzed after a 3-year follow-up of 25 OVL cases. The basal characteristics (age, gender and location) of all cases had no significant association with the types of lesions. Gingiva (39.4%) was the common site for all lesions. Distribution of the examined proteins had a significant association with the lesions. As compared with the OVLs, the number of immunostained-positive cells was significantly higher in the OVCs and lower in the OVH cases. During follow-up, 24% of the OVLs underwent malignant transformation for which high HuR expression and a diffuse staining pattern in the epithelium were observed. Taken together, the high degree of HuR expression with diffuse staining pattern in the epithelium may be an effective diagnostic tool that determines the potential of OVLs for malignant transformation. PMID:24534848

  17. Cytoplasmic expression of HuR may be a valuable diagnostic tool for determining the potential for malignant transformation of oral verrucous borderline lesions.

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Hida, Kyoko; Higashino, Fumihiro; Ohiro, Yoichi; Totsuka, Yasunori; Shindoh, Masanobu

    2014-04-01

    Oral verrucous carcinoma (OVC) is a low grade variant of oral squamous cell carcinoma, and oral verrucous hyperplasia (OVH) is a benign lesion without malignant features. However, pathologists are sometimes presented with borderline lesions and are indecisive as to diagnose them as benign or malignant. Thus, these lesions are tentatively termed oral verrucous lesions (OVLs). HuR is an ARE mRNA-binding protein, normally localized in the nucleus but cytoplasmic exportation is frequently observed in cancer cells. The present study aimed to elucidate whether expression of the HuR protein facilitates the diagnosis of true malignant lesions. Clinicopathological features were evaluated, and immunohistochemical analysis for p53, Ki67 and HuR proteins was performed in 48 cases of OVH, OVC and OVL, and the outcomes were correlated using appropriate statistical analysis. The association of these three proteins in relation to malignant transformation was analyzed after a 3-year follow-up of 25 OVL cases. The basal characteristics (age, gender and location) of all cases had no significant association with the types of lesions. Gingiva (39.4%) was the common site for all lesions. Distribution of the examined proteins had a significant association with the lesions. As compared with the OVLs, the number of immunostained-positive cells was significantly higher in the OVCs and lower in the OVH cases. During follow-up, 24% of the OVLs underwent malignant transformation for which high HuR expression and a diffuse staining pattern in the epithelium were observed. Taken together, the high degree of HuR expression with diffuse staining pattern in the epithelium may be an effective diagnostic tool that determines the potential of OVLs for malignant transformation.

  18. Interleukin 1β Regulation of the System xc- Substrate-specific Subunit, xCT, in Primary Mouse Astrocytes Involves the RNA-binding Protein HuR.

    Science.gov (United States)

    Shi, Jingxue; He, Yan; Hewett, Sandra J; Hewett, James A

    2016-01-22

    System xc(-) is a heteromeric amino acid cystine/glutamate antiporter that is constitutively expressed by cells of the CNS, where it functions in the maintenance of intracellular glutathione and extracellular glutamate levels. We recently determined that the cytokine, IL-1β, increases the activity of system xc(-) in CNS astrocytes secondary to an up-regulation of its substrate-specific light chain, xCT, and that this occurs, in part, at the level of transcription. However, an in silico analysis of the murine xCT 3'-UTR identified numerous copies of adenine- and uridine-rich elements, raising the possibility that undefined trans-acting factors governing mRNA stability and translation may also contribute to xCT expression. Here we show that IL-1β increases the level of mRNA encoding xCT in primary cultures of astrocytes isolated from mouse cortex in association with an increase in xCT mRNA half-life. Additionally, IL-1β induces HuR translocation from the nucleus to the cytoplasm. RNA immunoprecipitation analysis reveals that HuR binds directly to the 3'-UTR of xCT in an IL-1β-dependent manner. Knockdown of endogenous HuR protein abrogates the IL-1β-mediated increase in xCT mRNA half-life, whereas overexpression of HuR in unstimulated primary mouse astrocytes doubles the half-life of constitutive xCT mRNA. This latter effect is accompanied by an increase in xCT protein levels, as well as a functional increase in system xc(-) activity. Altogether, these data support a critical role for HuR in mediating the IL-1β-induced stabilization of astrocyte xCT mRNA.

  19. Type-Ⅰcannabinoid receptor mediates down-regulation of CXCR4 in astrocytes by cannabinoid HU210%Ⅰ型大麻素受体介导大麻素HU210对星型胶质细胞CXCR4水平下调

    Institute of Scientific and Technical Information of China (English)

    朱舟; 韩静; 张遐; 徐逸

    2016-01-01

    目的 探寻大麻素抑制中枢神经系统免疫反应的机制,为大麻素临床药物的合理应用提供实验依据.方法 用不同浓度的HU210刺激培养的星形胶质细胞,利用Western blot检测并比较刺激组和未刺激组细胞CXCR4蛋白水平的差异,进而用Ⅰ型大麻素受体(type-1 cannabinoid receptor,CB1R)拮抗剂AM281刺激细胞后,观察HU210对CXCR4表达的影响.结果 Western blot检测结果显示,高浓度HU210能下调星形胶质细胞CXCR4表达,AM28能阻断HU210所致的CXCR4下调.结论 HU210经由CB1R下调CXCR4,这可能是大麻发挥免疫抑制作用的机理之一.

  20. Administrative circulars No. 22A (rev. 1) – Award of additional periods of membership in the Pensions Fund for long-term shift work and No. 22B (rev. 1) – Compensation for long-term shift work hours

    CERN Multimedia

    HR Department

    2011-01-01

    Administrative Circulars No. 22A (Rev. 1) entitled "Award of additional periods of membership in the Pension Fund for long-term shift work" and No. 22B (Rev.1) entitled “Compensation for long-term shift work hours”, adopted following discussion in the Standing Concertation Committee meeting of 21 September 2010 and entering into force on 1 March 2011, are available on the intranet site of the Human Resources Department: http://cern.ch/hr-docs/admincirc/admincirc.asp They cancel and replace Administrative Circulars No. 22A and 22B entitled "Award of additional periods of membership in the Pension Fund to shift workers (Early Departure)” and “Duration and special compensation for shift work” of January 2000. This new version clarifies, in particular, the compensation of effective long-term shift work hours. Department Head Office  

  1. Resposta imunitária à vacinação conjuntival com a estirpe Rev.1 de Brucella melitensis em ovinos e caprinos Serological response of sheep and goats to conjunctival Brucella melitensis Rev.1 vaccine

    Directory of Open Access Journals (Sweden)

    P. Poeta

    2003-04-01

    Full Text Available The live B. melitensis Rev.1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants, especially when used at the standard dose by the conjunctival route. In the present study a 1´ 10(9 CFU dose for both sheep and goats conjunctivally vaccinated was tested to evaluate the duration of serological responses. Conjunctival vaccination with Rev. 1 performed in adult animals induced a rapid rise in serological titres as measured by Rose Bengal Plate Test (RBPT, Complement Fixation Test (CF and Modified Rose Bengal Plate Test (MRBPT. Titres then decreased and became negative in most animals by four months after vaccination (except MRBPT. The goats responded better to the vaccination than the sheep as one month after vaccination 100% of the goats revealed positive results to RB and RBM and 93.4% to FC test. The RBM was the one that detected more positive animals along the study.

  2. RevMan、Stata和R软件在生存资料Meta分析的应用%The Application of RevMan, Stata and R Software for Meta-analysis of Survival Data

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    生存资料的Meta分析越来越多.从原始文献计算出风险比(HR)及其95%可信区间,实际频数和理论频数的差值(O-E)及其标准差后,可以使用Review Manager (RevMan)、Stata和R等软件计算合并HR.RevMan软件简单易学,但存在一定的局限;Stata和R软件功能强大,灵活多样,能够绘制多种图形,但需要进行适当的编程.

  3. 无对照二分类数据的Meta分析在RevMan软件中的实现%Implement Meta-Analysis with Non-Comparative Binary Data in RevMan Software

    Institute of Scientific and Technical Information of China (English)

    陈月红; 杜亮; 耿兴远; 刘关键

    2014-01-01

    本文介绍无对照二分类数据资料的2种效应指标及其标准误的计算方法,然后通过1个实例分别用上述2种方法计算效应指标及其标准误,将计算所得的效应指标及其标准误在RevMan软件中进行Meta分析,最后将计算结果与用实例的原始数据在Stata软件中的Meta分析结果进行比较.结果显示RevMan和Stata软件进行无对照二分类数据资料Meta分析的结果一致.

  4. Comment on “Two-dimensional positive column structure in a discharge tube with radius discontinuity” [Phys. Plasmas 21, 113503 (2014)

    Energy Technology Data Exchange (ETDEWEB)

    Demidova, M. V. [Department of Chemistry, Wright State University, Dayton, Ohio 45435 (United States); Division of NLP Technology, INTEPH Technology LLC, Dayton, Ohio 45066 (United States); Kudryavtsev, A. A. [Division of NLP Technology, INTEPH Technology LLC, Dayton, Ohio 45066 (United States); International Laboratory “Nonlocal Plasma in Nanotechnology and Medicine”, ITMO University, Kronverkskiy pr. 49, St. Petersburg 197101 (Russian Federation); Kurlyandskaya, I. P. [International Laboratory “Nonlocal Plasma in Nanotechnology and Medicine”, ITMO University, Kronverkskiy pr. 49, St. Petersburg 197101 (Russian Federation); Department of Optics, St. Petersburg State University, St. Petersburg 199034 (Russian Federation); Saifutdinov, A. I.; Stepanova, O. M. [Department of Optics, St. Petersburg State University, St. Petersburg 199034 (Russian Federation)

    2015-09-15

    Zobnin et al. have published a paper [Phys. Plasmas, 21, 113503 (2014)] on a topic of discharge physics in the presence of a sharp change in cylindrical discharge geometry. In the comment it is pointed out that for untrapped electrons a full kinetic equation, which includes dependences on spatial coordinates and energies, has to be used for the electron velocity distribution function determination. It is also unclear what probe theories Zobnin et al. have used in their paper for the calculation of electron current to the discharge tube wall.

  5. Comment on “Competition between coherent emission and broadband spontaneous emission in the quantum free electron laser” [Phys. Plasmas 20, 033106 (2013)

    Energy Technology Data Exchange (ETDEWEB)

    Petrillo, V.; Rossi, A. R.; Serafini, L. [Università di Milano-INFN, Via Celoria, 16 Milano (Italy)

    2013-12-15

    We point out that in the equation for the electron distribution evolution during Thomson/Compton or undulator radiation used in the paper: “Competition between coherent emission and broadband spontaneous emission in the quantum free electron laser” by G. R. M. Robb and R. Bonifacio [Phys. Plasmas 20, 033106 (2013)], the weight function should be the distribution of the number of emitted photons and not the photon energy distribution. Nevertheless, the considerations expressed in this comment do not alter the conclusions drawn in the paper in object.

  6. Comment on ``Competition between coherent emission and broadband spontaneous emission in the quantum free electron laser'' [Phys. Plasmas 20, 033106 (2013)

    Science.gov (United States)

    Petrillo, V.; Rossi, A. R.; Serafini, L.

    2013-12-01

    We point out that in the equation for the electron distribution evolution during Thomson/Compton or undulator radiation used in the paper: "Competition between coherent emission and broadband spontaneous emission in the quantum free electron laser" by G. R. M. Robb and R. Bonifacio [Phys. Plasmas 20, 033106 (2013)], the weight function should be the distribution of the number of emitted photons and not the photon energy distribution. Nevertheless, the considerations expressed in this comment do not alter the conclusions drawn in the paper in object.

  7. Comment on “The two dimensional motion of a particle in an inverse square potential: Classical and quantum aspects” [J. Math. Phys. 54, 053509 (2013)

    Energy Technology Data Exchange (ETDEWEB)

    Bietenholz, Wolfgang, E-mail: wolbi@nucleares.unam.mx; Chryssomalakos, Chryssomalis, E-mail: chryss@nucleares.unam.mx; Salgado, Marcelo, E-mail: marcelo@nucleares.unam.mx [Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, A.P. 70-543, México D.F. 04510, México (Mexico)

    2015-10-15

    We comment on a fatal flaw in the analysis contained in the work of Martínez-y-Romero et al., [J. Math. Phys. 54, 053509 (2013)], which concerns the motion of a point particle in an inverse square potential, and show that most conclusions reached there are wrong. In particular, the manifestly senseless claim that, in the attractive potential case, no bounded orbits exist for negative energies, is traced to a sign error. Several more mistakes, both in the classical and the quantum cases, are pointed out.

  8. Comment on “Frequency-domain stimulated and spontaneous light emission signals at molecular junctions” [J. Chem. Phys. 141, 074107 (2014)

    Energy Technology Data Exchange (ETDEWEB)

    Galperin, Michael [Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093 (United States); Ratner, Mark A. [Department of Chemistry, Northwestern University, Evanston, Illinois 60208 (United States); Nitzan, Abraham [School of Chemistry, Tel Aviv University, Tel Aviv 69978 (Israel)

    2015-04-07

    We discuss the derivation of the optical response in molecular junctions presented by U. Harbola et al. [J. Chem. Phys. 141, 074107 (2014)], which questions some terms in the theory of Raman scattering in molecular junctions developed in our earlier publications. We show that the terms considered in our theory represent the correct contribution to calculated Raman scattering and are in fact identical to those considered by Harbola et al. We also indicate drawbacks of the presented approach in treating the quantum transport part of the problem.

  9. Comment on "Communication: Simple and accurate uniform electron gas correlation energy for the full range of densities" [J. Chem. Phys. 145, 021101 (2016)

    CERN Document Server

    Karasiev, Valentin V

    2016-01-01

    A simple expression for the uniform electron gas (UEG) correlation energy, recently presented in Ref. [J. Chem. Phys. 145, 021101 (2016)], deviates from the reference quantum Monte-Carlo (QMC) data at large r_s. We propose to define one of the parameters from a requirement to match the large-rs QMC data. Functional with the new parameter provides much better agreement with the QMC data at large r_s without deterioration of the functional quality at small and intermediate r_s.

  10. Response to "Comment on 'Rethinking first-principles electron transport theories with projection operators: the problems caused by partitioning the basis set'" [J. Chem. Phys. 140, 177103 (2014)].

    Science.gov (United States)

    Reuter, Matthew G; Harrison, Robert J

    2014-05-01

    The thesis of Brandbyge's comment [J. Chem. Phys. 140, 177103 (2014)] is that our operator decoupling condition is immaterial to transport theories, and it appeals to discussions of nonorthogonal basis sets in transport calculations in its arguments. We maintain that the operator condition is to be preferred over the usual matrix conditions and subsequently detail problems in the existing approaches. From this operator perspective, we conclude that nonorthogonal projectors cannot be used and that the projectors must be selected to satisfy the operator decoupling condition. Because these conclusions pertain to operators, the choice of basis set is not germane.

  11. ADMINISTRATIVE CIRCULAR No. 25 (REV. 3) - Special provisions for the fire and rescue service governing working and rest time

    CERN Document Server

    2012-01-01

    Administrative Circular No. 25 (Rev. 3) entitled “Special provisions for the Fire and Rescue Service governing working and rest time”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 28 September 2012 and entering into force in October 2012, is available on the intranet site of the Human Resources Department: http://cern.ch/hr-docs/admincirc/admincirc.asp This Circular is applicable to staff members of the Fire and Rescue Service It cancels and replaces Administrative Circular No. 25 (Rev. 2) entitled “Shift work – Special provisions for the Fire and Rescue Service” of April 2003. This new version takes into account the new organisation of the Fire and Rescue Service, members of which will henceforth not exclusively perform their functions in the context of shift work, but also during reference working hours and during stand-by duty. Additionally, applicable limits regarding working and rest times an...

  12. Operational Circular No.2 (Rev. 2) - Conditions of access to the fenced parts of the CERN site

    CERN Multimedia

    2014-01-01

    Operational Circular No. 2 (Rev. 2) entitled “Conditions of access to the fenced parts of the CERN site” and its “implementation measures”, approved by the Director-General following discussion at the Standing Concertation Committee meeting on 20 May 2014 and entering into force on 1 September 2014, are available on the intranet site of the Human Resources Department (see here).   This circular is applicable to members of the personnel and other persons concerned. It cancels and replaces Operational Circular No. 2 (Rev. 1) entitled “Conditions of access to the fenced parts of the CERN site”, of April 1998. In particular, the revised circular provides for the possibility of mandating a person responsible for the proper implementation of the circular, specifies the rules relating to vehicles allowed on the site and the respective responsibilities of their owners, and relaxes certain administrative formalities in case of loss, theft or di...

  13. Operational Circular No. 2 (Rev. 3) - Conditions of access to the fenced parts of the CERN site

    CERN Multimedia

    HR Department

    2015-01-01

    Operational Circular No. 2 (Rev. 3) entitled "Conditions of access to the fenced parts of the CERN site", approved by the Director-General following discussion in the Standing Concertation Committee meeting on 24 September 2015, is now available via this link.   This revised circular cancels and replaces Operational Circular No. 2 (Rev. 2) also entitled "Conditions of access to the fenced parts of the CERN site", of September 2014. The circular was revised predominantly in order to specify that access to the CERN site is granted to CERN Pension Fund beneficiaries only provided that they are actually in receipt of payments from the Fund; and to allow the Director-General to permit special types of vehicles on site, such as trailers. It also includes a certain number of text improvements and an updated version of the implementation measures, in particular with regard to vehicle identification, road traffic and parking.  

  14. Administrative circular n°3 (Rev. 2) – Home leave, travel to home station and assimilated leave and travel

    CERN Multimedia

    2013-01-01

    Administrative Circular No. 3 (Rev. 2) entitled “Home leave, travel to the home station and assimilated leave and travel”, approved by the Director-General following discussion at the Standing Concertation Committee meeting of 11 October 2012 and entering into force on 1 January 2013, is available on the intranet site of the Human Resources Department.   This circular is applicable to employed members of the personnel. It cancels and replaces Administrative Circular No. 3 (Rev. 1) entitled “Travel to the home station and home leave” of June 2002. The circular was revised in order to take into account the new status of Associate Member State and the fact that henceforth, home stations may be situated on territory outside of Europe. It is proposed to introduce a new system of determination of the benefits (Travel expenses, travel time and distance indemnity) granted in the context of home leave and supplementary journeys to the home station.  For t...

  15. LabINRS. Mesures en laboratoire du coefficient de frottement dynamique de revêtements de sol

    OpenAIRE

    Marchal, P.; Jacques, M.

    2014-01-01

    Le LabINRS est un banc d’essai de laboratoire destiné à mesurer le coefficient de frottement dynamique d’un revêtement de sol. Ce document décrit le protocole de mesures avec le LabINRS et les résultats de mesures effectuées sur un panel de quinze revêtements de sol de caractéristiques différentes (matériaux, états de surface) réalisées en présence de deux polluants (huile et solution d’eau). Le LabINRS présente une sensibilité élevée et une très bonne répétabilité. Ces résultats ont été comp...

  16. Final Report - Sulfate Solubility in RPP-WTP HLW Glasses, VSL-06R6780-1, Rev. 0

    Energy Technology Data Exchange (ETDEWEB)

    Kruger, Albert A.; Pegg, I. L.; Feng, A.; Gan, H.; Kot, W. K.

    2013-12-03

    This report describes the results of work and testing specified by Test Specifications 24590-HLW-TSP-RT-01-006 Rev 1, Test Plans VSL-02T7800-1 Rev 1 and Test Exceptions 24590-HLW-TEF-RT-05-00007. The work and any associated testing followed established quality assurance requirements and were conducted as authorized. The descriptions provided in this report are an accurate account of both the conduct of the work and the data collected. Results required by the Test Plans are reported. Also reported are any unusual or anomalous occurrences that are different from the starting hypotheses. The test results and this report have been reviewed and verified.

  17. A new Method to Estimate the Representative Elementary Volume (REV) for Porosity in Heterogeneous Karst Aquifers Using Geographic Information Systems

    Science.gov (United States)

    Gross, M. R.; Manda, A. K.

    2004-12-01

    Karst limestones are characterized by solution-enhanced macropores and conduits that lead to exceptional heterogeneity at the aquifer scale. The interconnected network of solution cavities often results in a conduit flow regime that bypasses the less permeable rock matrix. Efforts to manage and protect karst aquifers, which are vital water resources in many parts of the world, will benefit from meaningful characterizations of the heterogeneity inherent in these formations. To this end, we propose a new method to estimate the representative elementary volume (REV) for macroporosity within karst aquifers using techniques borrowed from remote sensing and geospatial analysis. The REV represents a sampling window in which numerous measurements of a highly-variable property (e.g., porosity, hydraulic conductivity) can be averaged into a single representative value of statistical and physical significance. High-resolution borehole images are classified into binary images consisting of pixels designated as either rock matrix or pore space. A two-dimensional porosity is calculated by summing the total area occupied by pores within a rectangular sampling window placed over the binary image. Small sampling windows quantify the heterogeneous nature of porosity distribution in the aquifer, whereas large windows provide an estimate of overall porosity. Applying this procedure to imagery taken from the Biscayne aquifer of south Florida yields a macroporosity of ~40%, considerably higher than the ~28% porosity measured from recovered core samples. Geospatial analysis may provide the more reliable estimate because it incorporates large solution cavities and conduits captured by the borehole image. The REV is estimated by varying the size of sampling windows around prominent conduits and evaluating the change in porosity as a function of window size. Average porosities decrease systematically with increasing sampling size, eventually converging to a constant value and thus

  18. Nuclear receptor REV-ERBα mediates circadian sensitivity to mortality in murine vesicular stomatitis virus-induced encephalitis.

    Science.gov (United States)

    Gagnidze, Khatuna; Hajdarovic, Kaitlyn H; Moskalenko, Marina; Karatsoreos, Ilia N; McEwen, Bruce S; Bulloch, Karen

    2016-05-17

    Certain components and functions of the immune system, most notably cytokine production and immune cell migration, are under circadian regulation. Such regulation suggests that circadian rhythms may have an effect on disease onset, progression, and resolution. In the vesicular stomatitis virus (VSV)-induced encephalitis model, the replication, caudal penetration, and survivability of intranasally applied VSV depends on both innate and adaptive immune mechanisms. In the current study, we investigated the effect of circadian time of infection on the progression and outcome of VSV-induced encephalitis and demonstrated a significant decrease in the survival rate in mice infected at the start of the rest cycle, zeitgeber time 0 (ZT0). The lower survival rate in these mice was associated with higher levels of circulating chemokine (C-C motif) ligand 2 (CCL2), a greater number of peripherally derived immune cells accumulating in the olfactory bulb (OB), and increased production of proinflammatory cytokines, indicating an immune-mediated pathology. We also found that the acrophase of molecular circadian clock component REV-ERBα mRNA expression in the OB coincides with the start of the active cycle, ZT12, when VSV infection results in a more favorable outcome. This result led us to hypothesize that REV-ERBα may mediate the circadian effect on survival following VSV infection. Blocking REV-ERBα activity before VSV administration resulted in a significant increase in the expression of CCL2 and decreased survival in mice infected at the start of the active cycle. These data demonstrate that REV-ERBα-mediated inhibition of CCL2 expression during viral-induced encephalitis may have a protective effect.

  19. Modelo de gestión cultural: Una experiencia con el Qhapaq Ñan en Huánuco

    Directory of Open Access Journals (Sweden)

    Denesy Palacios Jiménez

    2014-06-01

    Full Text Available Objetivos: Plantear el aprovechamiento del patrimonio cultural arqueológico del Qhapaq Ñan en Huánuco en condición de parque cultural, mediante un modelo eficiente de gestión cultural basada en la investigación, protección, conservación y uso social, para convertir a este lugar en un destino turístico y así contribuir con un proyecto no solo para salvaguardarlo sino también para incrementar los ingresos económicos y reducir los niveles de pobreza en la zona. Métodos: Se trata de un estudio que describe y recoge experiencias de gestión cultural respecto a cómo se aprovecha en otros sitios el legado o patrimonio cultural arqueológico, tanto en el ámbito mundial como nacional. La investigación fue cuantitativa y cualitativa, con un alcance correlacional. Se usó el diseño descriptivo, comparativo, analítico, histórico, deductivo. Resultados: El estudio plantea la necesidad de optimizar los recursos para el aprovechamiento del Qhapaq Ñan como parque cultural a través de una adecuada gestión cultural, y que dicho parque comprenderá 68,34 km, desde el tambo de Tambococha o Tunsucancha hasta el tambo de Taparako, por ser uno de los tramos mejor conservados e involucra importantes zonas de patrimonio cultural material e inmaterial. Conclusiones: El Camino Inca en Huánuco es uno de los patrimonios más importantes del país, y es posible ser impulsado como un parque cultural mediante un modelo de gestión cultural, si tenemos en cuenta las diversas experiencias de gestión en el país y en mundo, cuyos aportes deben servir de apoyo para un modelo de gestión en este campo.

  20. The Clock Gene Rev-Erbα Regulates Methamphetamine Actions on Circadian Timekeeping in the Mouse Brain.

    Science.gov (United States)

    Salaberry, Nora L; Mateo, Maria; Mendoza, Jorge

    2017-09-01

    Circadian rhythms are strongly affected by drugs. In rodents, chronic methamphetamine (METH) intake changes circadian activity rhythms, mainly by altering light synchronization that generates the expression of a free-running rhythm with a period longer than 24 h and a second behavioral component that is independent of the main suprachiasmatic (SCN) clock. Although a number of clock genes do not appear to be involved in the effects of METH on circadian behavior, the molecular clockwork controlling these changes is still unclear. Therefore, we investigated the role of the clock gene Rev-Erbα in METH-induced behavioral and molecular responses using knockout mice and their wild-type littermates. Chronic intake of METH alters period circadian behavior of wild-type mice. However, in mice lacking the clock gene Rev-Erbα METH had no effect on their behavioral rhythms. Furthermore, PER2 bioluminescence rhythms in two extra-SCN brain oscillators, the dorsomedial hypothalamus and the habenula, were altered by METH in wild type but not in KO mice. Together, the present results implicate Rev-Erbα in the modulation of the circadian responses to METH and may provide a better comprehension into the mechanisms underlying circadian alterations provoked by drug addiction.