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Sample records for hu feng counter-revolutionary

  1. Hu Qiaomu and Hu Feng Event%胡乔木与胡风案

    Institute of Scientific and Technical Information of China (English)

    张成洁

    2015-01-01

    It had a gradual upgrade process on a critique of Hu Feng's literary thoughts and a political settlement to Hu Feng.As the senior vice minister of the Propaganda Department of the Central Committee of the CPC,Hu Qiaomu played an important role on it.In October 1945,Hu Qiaomu still stayed in Chungking after Chungking Negotiations and counter-acted the effects of Hu Feng's literary thoughts by criticizing"subjectiveness".Public criticism to Hufeng launched by"popular literature series" was supported by Hu Qiaomuin in Hongkong in 1948.The first two years of the founding of new China,Hu Qiaomu talked with Hufeng for many times to solve his ideological problems.Hu Qiaomu also organized criticism of Hu Feng's literary thoughts throught the opportunity of Shuwu self-criticism;Hu Qiaomu first mentioned publicly"a small cabal of literary headed by Hufeng"In his giving editorial notes of Shuwu's article.Hu Feng's private let-ters to Shuwu were disclosed which directly led to Hu Feng event upgrading to"anti-party and anti-revolution"problems in the process of the great criticism of Hu Feng.After 1954,this matter had an indirect relationship with Hu Qiaomu's suggest of writing Hu Feng sectarianism.However,Hu Qiaomu disapproved to determine Hu Feng and the people impli-cating with Hu Feng as"Hufeng Anti-Party Clique".He gave a claim in this connection before Mao Zedong.In the past, people very paid attention to Zhouyang who had deep historical grievances with Hu Feng and ignored the role of Hu Qiao-mu in Hu Feng event.%对胡风文艺思想的批判和对胡风的政治清算有一个渐次升级的过程。其中,在对胡风文艺思想的批判中,胡乔木起了重要作用。1945年10月,胡乔木随毛泽东去重庆谈判后逗留重庆,借批《论主观》来清除胡风文艺思想的影响;1948年香港的《大众文艺丛刊》对胡风发起的公开批评,也得到了胡乔木的支持;新中国成立头两年,胡乔木多次奉命找胡风谈话

  2. Difference Existed Is Difficult to Obtain Agreement: Hu Feng's View on May 4th Different from the Mainstream and Its Influence on Hu Feng's Doom%“存异”难以“求同”——胡风“五四”观异于主流的歧见及其对胡风命运的影响

    Institute of Scientific and Technical Information of China (English)

    谢刚

    2012-01-01

    如何看待“五四”,是审视胡风思想内质及其与左翼主流分歧的一个关键角度。两者在“五四”总主题的认识上并无差异,而在“五四”领导者的认定及历史意义的阐发上却有不同。胡风认为:经过真切实践的鲁迅在“五四”时期已经成为共产主义知识分子.理当居于“五四”领导者之列,这与权威的“鲁迅转变论”产生龃龉;“五四”的历史意义主要在于文学/文化层面,而非政治范畴。“五四”的反封建传统较之其反帝传统更为根本和重要。这些独异之见动摇了主流意识形态合法性论证的逻辑,是导致胡风悲剧命运的重要祸根。%How to look on May 4th is key point that surveys Hu Feng's thought and its divergence with Left Wing's main thought. Both had no difference on cognition of main thesis of May 4th but difference on leader and historical significance of May 4th, Hu Feng argued that Lu Xun had become communist through personal experience in May 4th period and should become a leader of May 4th. The view had discord with authoritative conclusion about Lu Xun change. Hu Feng thought that historical significance of May 4th focused on literature or culture not on political category and anti-feudalism was more fundamental and important than anti-imperialism tradition. These unique views shook demonstrative logic of legitimacy of the mainstream ideology, which led to Hu Feng's tragic doom.

  3. Enlightenment and Sensing:Hu Feng's Literary Thought Associated with Lu Xun's Spirit%启蒙与实感:胡风文艺思想与鲁迅传统的关联

    Institute of Scientific and Technical Information of China (English)

    蔡洞峰

    2015-01-01

    胡风的文艺思想通过其在现实的实践斗争中形成了启蒙现实主义理论体系.胡风继承了鲁迅的文学"为人生"的"五四"启蒙精神,将启蒙思想纳入到他的"主观战斗精神"、"大众化"等理论体系中,并通过文学的"实感"使启蒙思想通过文学的方式得以实现.总体说来,通过文学实现五四启蒙精神,并建构起启蒙现实主义的理论是胡风传承鲁迅精神的实质和核心.%Hu Feng's literary thought is the enlightenment theory of realism formed through his practical struggle. Inherited from Lu Xun's "May 4th"spirit of enlightenment in literature "for life", Hu Feng brought the enlightenment thought into his theoretical system of "Subjective fighting spirit" and "Popularity", and had the enlightenment thought come into being by literary"sensing" in literature. Generally speaking, the essence and core of Hu Feng's inheritance from Lu Xun's spirit is to realize "May 4th"spirit of enlightenment in literature, and subsequently to form his own enlightenment theory of realism

  4. Revolutionary in Counter-Revolutionary Times: Elaborating Fanonian National Consciousness into the Twenty-First Century

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    Jane Anna Gordon

    2011-06-01

    Full Text Available One of the unique challenges of reading Les damnés de la terre (The Wretched of the Earth today is that while it is an irredeemably revolutionary text, we live in a counter-revolutionary moment or in a global context that has tried very hard to discredit even the possibility of revolution. Fanon’s text does not only narrate the effective undertaking of an anti-colonial struggle—of what is required for people to identify the actual causes of their alienation and unfreedom and together to will their elimination—it also outlines the various, often dialectical challenges of restructuring a society from the bottom up. Guiding and evident in the latter is the flourishing of what Fanon suggestively called national consciousness. Elaborating its meaning and ongoing usefulness is the focus of this essay.

  5. “展缓判断”求真相:冯友兰与胡适对孔老先后之争的学术意义--兼对冯友兰复胡适信函时间考证%Suspension of Judgement:The Academic Significance of Debate Between Feng Youlan and Hu Shi ---Textual Research on Time of Feng Youlan and Hu Shi’s Letters

    Institute of Scientific and Technical Information of China (English)

    张婷

    2014-01-01

    Early 30s of the last century, Feng Youlan and Hu Shi had several debates on Confucius and Laozi. Each side held dif-ferent opinons from his own ideological analysis and textual research. After nearly half a century, finally under the support of the un-earthed cultural relics, Hu Shi was proved to be true. His“Suspension of judgment” academic principles on issues of the present academ-ic research has great significance.%20世纪30年代初,冯友兰与胡适等人针对老子其人其书与孔子的先后问题,曾经展开过多次交锋。双方各自从所擅长的思想系统分析和考证方法辨伪等几个方面进行讨论,意见迭出。近半个世纪后,最终在出土文物的佐证下,胡适生前的推断被证明为真。他所力倡的“展缓判断”学术原则及对争议问题的处理态度对当下的学术研究具有很大的启示意义。

  6. "酒"与"山水之间"和"醉翁之意"——胡风与周扬恩怨是非新论%"Wine", "the Scenery of Mountains and Rivers" and the Intention of "the Drunkard":New Discussion of Gratitude or Resentment between Zhou Yang and Hu Feng for Several Dozens Years

    Institute of Scientific and Technical Information of China (English)

    李乐平

    2011-01-01

    Many scholars always think that injustice case of Fufeng has a quite big negative function in the feeling of gratitude or resentment between Zhou Yang and Fu Feng. It cannot be denied that Zhou Yang played an irreplaceable function in injustice case of Fufeng and even the rehabiliation process of the case. However, dozens of years' contradiction between Hu Feng and Zhou Yang, and even "the indictment" which Hu Feng accused to Zhou Yang, all belongs to the category of Thought on Literary and Arts. We shall acknowledge the measures and the attitude which Zhou Yang dealt with the issues of Fu Feng, that's not "A wine lover's mind which is not dipped in the bobbled, but dwells in nature", but is the meaning of "the drunkard" who draws support from "the scenery of mountains and rivers" to complete the intention of drinking "wine". Hu Feng's disaster is a tragedy of that time, and its significance exceeds his own destiny completely. Therefore, the theory of literature and arts cannot be tied up together with politics again. It's also the consideration left to posterity. In this way can avoid injustice of disaster because of a personal grudge. If we can't go outside and continue to stand in the "Left" situation to animadvert the loop of strange mighty "Left", we would not be able to be practical and realistic on the fundamental problems in literary, and not to mention to recover the pursuit of human liberation of the spirit of "Wusi", and to recover hominine literature, in particular, the historical tradition of democracy and science.%很多学者总认为周扬和胡风几十年的恩怨在胡风冤案中起了很大负面作用,但胡风和周扬几十年的矛盾乃至他所指控周扬的"罪状"都属于当时主流文艺思想的范畴.周扬在处理胡风问题上所采取的措施和态度,不是"醉翁之意不在酒,在于山水之间",而是"醉翁"借助"山水之间"行"酒"之意.胡风的舛难是一个时代的悲剧,其意义完全超越他个人命运,

  7. Connotation of the Enlightenment Discourse and Debate about“National Form” in Literature and Art from Perspective of HU Feng s Doubt%启蒙话语交锋与“民族形式”文艺论争的实际内涵--以胡风的质疑为视角

    Institute of Scientific and Technical Information of China (English)

    毕海

    2013-01-01

    The debate about “national form” in literature and art around 1940 s involved a series of theo-retical issues such as how to use old form, and the appraisement of“May 4th Movement”, etc. HU Feng, who had analyzed and questioned the debate, not only criticized the viewpoint of the Popular Reading Publishing House,i. e. ,“new character was born out of the‘old character’”, but also disagreed with other left-wing writ-ers. He was actually adhering to the May 4th Enlightened Literature Tradition initiated by LU Xun. The fact that HU Feng was criticized indicates that the“National Form” literature movement was intertwined with politi-cal ideology, which marked the beginning of the intellectuals. destiny of being transformed.%20世纪40年代前后文艺的“民族形式”论争,涉及了如何利用旧形式、评价“五四”新文学等一系列的理论问题。胡风对“民族形式”论争的分析,既批评了通俗读物编刊社“新质发生于旧质的胎内”的观点,也与其他左翼作家的意见不同,实际上是坚持鲁迅所开创的“五四”启蒙文学传统。胡风的质疑和被批判,显示了“民族形式”运动文学与政治意识形态纠缠的实际内涵,同时也标志着知识分子被改造命运的开始。

  8. The counter-revolutionary myth of the «Restauration» | El mito contrarrevolucionario de la «Restauración»

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    Pedro Rújula

    2014-12-01

    Full Text Available During the Peninsular war, the ultra-realist sectors put forward the view that the basis of the old order had been attacked, and that its foundations had been destroyed. Widespread press activity promoting this view flourished. However, just before the return of the king in 1814, these sectors envisaged an opportunity to recover lost power and altered their strategy. The realists turned the myth of the recovery of the lost order through the restoration of the Bourbon dynasty into an instrument intended to deny the relevance of previous changes. This essay aims to explain the role performed by the construction of the restoration myth in supporting the counter-revolutionary coup on May 1814. | Durante la guerra de la independencia los sectores ultra-realistas construyeron la imagen de que el mundo anterior había sido atacado en sus bases y destruidos los elementos que lo sustentaban. Una amplia publicística fue creciendo en este contexto. Sin embargo, ante el inminente regreso del rey en 1814, vieron la oportunidad de recuperar el poder perdido y cambiaron de estrategia. El mito de la recuperación del orden perdido mediante la restauración de los Borbones va a ser el instrumento del que los realistas se valdrán para negar la magnitud de los cambios vividos durante aquellos años. El presente artículo intenta explicar la importancia que tuvo la construcción del mito de la restauración para sustentar el golpe contrarrevolucionario de mayo de 1814.

  9. Feng Shui dalam Mendesain Logo

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    Monica Monica

    2011-04-01

    Full Text Available A good logo is a reflection of a corporate identity, vision and mission, as well as a summary of the company's character. Feng shui is the ancient Chinese science of topography is believed to affect the success of making a good logo. According to Feng shui, every color, shape, letters, and numbers can affect the energy contained in the company logo. And these factors also have a grouping of the elements of each, a case of elements of earth, metal, fire, wood, and water. If the combination of the elements is wrong, it will create a destructive energy flowing so that hamper the pace of corporate fortunes. With the approach of the study and literature review, this article will explain how the relationship between the selection of colors, shapes, letters, and numbers in accordance with Feng Shui and may still have some artistic value.  

  10. Feng Shui dalam Mendesain Logo

    Directory of Open Access Journals (Sweden)

    Monica

    2011-03-01

    Full Text Available A good logo is a reflection of a corporate identity, vision and mission, as well as a summary of the company's character. Feng shui is the ancient Chinese science of topography is believed to affect the success of making a good logo. According to Feng shui, every color, shape, letters, and numbers can affect the energy contained in the company logo. And these factors also have a grouping of the elements of each, a case of elements of earth, metal, fire, wood, and water. If the combination of the elements is wrong, it will create a destructive energy flowing so that hamper the pace of corporate fortunes. With the approach of the study and literature review, this article will explain how the relationship between the selection of colors, shapes, letters, and numbers in accordance with Feng Shui and may still have some artistic value.

  11. Landscape Design and Feng-Shui

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    L. Erdinc

    2009-09-01

    Full Text Available Feng-shui is a comprehensive concept dealing with design professions such as city and regional planning, architecture, indoor design and landscape architecture as a design phenomenon. It is also a design concept including space design principles whose main design aims are observing nature to ensure balance with nature and conserving nature by designing harmonies environments with nature. These principles make feng-shui extracted only from being hobby, mystical belief or art. Many actual design approaches such as sustainable design, ecological settlements, ecological planning and design were based on this philosophy which appeared centuries ago in the Far East. The main aim of this study is to evaluate the theoretical base and the implementations of feng-shui and to determine the ideal feng-shui models in open green space design approaches. At the first stage conceptual explanation about feng-shui and the development of feng-shui through history was set. At the second stage experiences and application at international stage were discussed. And, finally the usage of feng-shui in landscape architecture design was evaluated and proposals were offered.

  12. Feng shui viis elementi / Ann Tenno

    Index Scriptorium Estoniae

    Tenno, Ann, 1952-

    2003-01-01

    Feng shui õpetuse kohaselt on viie põhielemendi vahelised suhted vastastikku loovad või hävitavad ja elementide kasutamisel oma isikliku ruumi kujundamisel tuleb arvestada eelkõige elementide omavahelisi seoseid

  13. Feng shui viis elementi / Ann Tenno

    Index Scriptorium Estoniae

    Tenno, Ann, 1952-

    2003-01-01

    Feng shui õpetuse kohaselt on viie põhielemendi vahelised suhted vastastikku loovad või hävitavad ja elementide kasutamisel oma isikliku ruumi kujundamisel tuleb arvestada eelkõige elementide omavahelisi seoseid

  14. Genetic determination of the enhanced drought resistance of rice maintainer HuHan2B by pedigree breeding.

    Science.gov (United States)

    Wei, Haibin; Feng, Fangjun; Lou, Qiaojun; Xia, Hui; Ma, Xiaosong; Liu, Yunhua; Xu, Kai; Yu, Xinqiao; Mei, Hanwei; Luo, Lijun

    2016-11-17

    The ongoing deficit of fresh water resource in rice growing regions has made the selection of water-saving and drought-resistance rice (WDR) a crucial factor in developing sustainable cultivation. HuHan2B, a new japonica maintainer for WDR breeding, had the same yield potential as recurrent parent HanFengB but showed improved drought resistance in fields. We investigated the genomic content accumulation and candidate genes passed from parent to offspring using the genomic and transcriptomic approaches. The genomic constitution indicated that the genetic similarity was 84% between HuHan2B and HanFengB; additionally, 7,256 genes with specific alleles were inherited by HuHan2B from parents other than HanFengB. The differentially expressed genes (DEGs) under drought stress showed that biological function was significantly enriched for transcript regulation in HuHan2B, while the oxidation-reduction process was primarily enriched in HanFengB. Furthermore, 36 DEGs with specific inherited alleles in HuHan2B were almost involved in the regulatory network of TFs and target genes. These findings suggested that major-effect genes were congregated and transformed into offspring in manner of interacting network by breeding. Thus, exploiting the potential biological function of allelic-influencing DEGs would be of great importance for improving selection efficiency and the overall genetic gain of multiple complex traits.

  15. Feng shui põhiprintsiibid - ajastamine / Ann Tenno

    Index Scriptorium Estoniae

    Tenno, Ann, 1952-

    2003-01-01

    Mida mõistetakse ajastamise all feng shui's. Feng shui abil endale soodsa elukoha leidmisel tuleb silmas pidada järgnevaid põhielemente: chi energia, mäed, vesi, tuul, maapind, suund ja orientatsioon ning ajastamine

  16. Feng shui põhiprintsiibid - ajastamine / Ann Tenno

    Index Scriptorium Estoniae

    Tenno, Ann, 1952-

    2003-01-01

    Mida mõistetakse ajastamise all feng shui's. Feng shui abil endale soodsa elukoha leidmisel tuleb silmas pidada järgnevaid põhielemente: chi energia, mäed, vesi, tuul, maapind, suund ja orientatsioon ning ajastamine

  17. Weierstrass semigroups and the Feng-Rao Distance

    DEFF Research Database (Denmark)

    Campillo, Antonio; Farran, Ignacio

    2000-01-01

    We detrmine the Feng-Rao distance for several claases of codes from algebraic geometry usingthe weierstrass semigroups......We detrmine the Feng-Rao distance for several claases of codes from algebraic geometry usingthe weierstrass semigroups...

  18. Feng shui taevased olendid / Ann Tenno

    Index Scriptorium Estoniae

    Tenno, Ann, 1952-

    2003-01-01

    Traditsioonilise maastikukoolkonna feng shui sümboliteks on roheline draakon, valge tiiger, must kilpkonn, punane fööniks. Taevased olendid tähistavad eelkõige maastikuvorme, nende abil võib tähistada olemasolevaid ehitisi ja struktuure, mis ümbritsevad meie kodu

  19. Feng shui taevased olendid / Ann Tenno

    Index Scriptorium Estoniae

    Tenno, Ann, 1952-

    2003-01-01

    Traditsioonilise maastikukoolkonna feng shui sümboliteks on roheline draakon, valge tiiger, must kilpkonn, punane fööniks. Taevased olendid tähistavad eelkõige maastikuvorme, nende abil võib tähistada olemasolevaid ehitisi ja struktuure, mis ümbritsevad meie kodu

  20. A remembrance of Professor TP FENG

    Institute of Scientific and Technical Information of China (English)

    Richard W. Tsien

    2007-01-01

    @@ It is both an honor and pleasure to be given this opportunity to remember Professor TP FENG and his many contributions.He made many important scientific discoveries,labored mightily to establish firmly Physiology and Neuroscience in China,and was a major force in setting a high standard for scientific inquiry.

  1. Feng Yun-A higher Class

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    TO this Weiqi player, rising to a higher class is like climbing a mountain. Hangzhou city is a lucky place for Feng Yun for it is where she won her first national championship 10 years ago. Commonly known by it's Japanese name "Go", Weiqi is actually of Chinese origin and is a game of

  2. On the Feng-Rao bound for generalized hamming weights

    DEFF Research Database (Denmark)

    Geil, Hans Olav; Thommesen, Christian

    2006-01-01

    The Feng-Rao bound gives good estimates of the minimum distance of a large class of codes. In this work we are concerned with the problem of how to extend the Feng-Rao bound so that it deals with all the generalized Hamming weights. The problem was solved by Heijnen and Pellikaan in [7] for a lar...

  3. On the Feng-Rao bound for generalized Hamming weights

    DEFF Research Database (Denmark)

    Geil, Olav; Thommesen, Christian

    2005-01-01

    The Feng-Rao bound gives good estimates of the minimum distance of a large class of codes. In this work we are concerned with the problem of how to extend the Feng-Rao bound so that it deals with all the generalized Hamming weights. The problem was solved by Heijnen and Pellikaan in [7] for a large...... family of codes that includes the duals of one-point geometric Goppa codes and the q-ary Reed-Muller codes, but not the Feng-Rao improved such ones. We show that Heijnen and Pellikaan’s results holds for the more general class of codes for which the traditional Feng-Rao bound can be applied. We also...... establish the connection to the Shibuya-Sakaniwa bound for generalized Hamming weights ([15], [16], [17], [18], [19] and [20]). More precisely we show that the Shibuya-Sakaniwa bound is a consequence of the extended Feng-Rao bound. In particular the extended Feng-Rao bound gives always at least as good...

  4. Keeping Current: Feng Shui and the School Library Media Specialist.

    Science.gov (United States)

    Barron, Daniel D.

    2000-01-01

    Discusses the changing shift in education, including learning resources centers, from a production to a consumer mind-set, focusing on the activity of learning rather than teaching. Highlights include learning communities; personal mastery; developing a vision; the interconnectedness of organizations; harmony and balance, similar to feng shui; and…

  5. Eestimaine feng shui talus ja mõisas / Kadi Lehtmets

    Index Scriptorium Estoniae

    Lehtmets, Kadi

    2006-01-01

    Geobioloogia ja feng shui asjatundjad Siret ja Janno Seeder on uurinud ja mõõtnud Eesti talukohtade ja mõisate energeetikat. Ilmneb, et on osatud end loodusenergiatega tasakaalu seada. S. ja J. Seederi nõuandeid uue maja ehitajaile

  6. Keeping Current: Feng Shui and the School Library Media Specialist.

    Science.gov (United States)

    Barron, Daniel D.

    2000-01-01

    Discusses the changing shift in education, including learning resources centers, from a production to a consumer mind-set, focusing on the activity of learning rather than teaching. Highlights include learning communities; personal mastery; developing a vision; the interconnectedness of organizations; harmony and balance, similar to feng shui; and…

  7. Eestimaine feng shui talus ja mõisas / Kadi Lehtmets

    Index Scriptorium Estoniae

    Lehtmets, Kadi

    2006-01-01

    Geobioloogia ja feng shui asjatundjad Siret ja Janno Seeder on uurinud ja mõõtnud Eesti talukohtade ja mõisate energeetikat. Ilmneb, et on osatud end loodusenergiatega tasakaalu seada. S. ja J. Seederi nõuandeid uue maja ehitajaile

  8. Dr. Feng Xinghua's Experience in Treating Ankylosing Spondylitis

    Institute of Scientific and Technical Information of China (English)

    Liu Hongxiao

    2006-01-01

    @@ Ankylosing spondylitis (AS) is a rheumatic disease mainly involving the axial joints, characterized by sacro-iliilis and rigid spine. Dr. Feng Xinghua from Guang'anmen Hospital affiliated to China Academy of Chinese Medical Sciences has been engaged in the clinical research and treatment of arthralgia syndrome for more than 30 years. His experience is summarized as follows.

  9. FENG SHUI IN MODERN HOUSE DESIGN SEARCHING FOR THE RATIONALE AND POSSIBLE IMPACTS ASSESSMENT

    Directory of Open Access Journals (Sweden)

    OCTAVIA Linda

    2014-07-01

    Full Text Available The Feng Shui, the "wind-water", is a Chinese system of geomancy using the laws of both Heaven and Earth for harmony and balance of life with the positive qi. Feng Shui is widely used to orient temples, palaces, shops, houses, and other structures, with the reference to local features such as bodies of water, stars, or a compass. The Feng Shui also informs the physical arrangements for manipulation and channeling the qi (positive or negative energy. Although Feng Shui is related to traditional believe and propitious manner, but some of its principles are rational. The current research is conducted to investigate any positive impacts of the Feng Shui application in modern house design. A house in Jakarta designed with Feng Shui was analyzed. The strategies were later examined based on the sustainable architectural design principles, especially its impact on the house functionality.

  10. Fast Erasure and Error decoding of Algebraic Geometry Codes up to the Feng-Rao Bound

    DEFF Research Database (Denmark)

    Jensen, Helge Elbrønd; Sakata, S.; Leonard, D.

    1996-01-01

    This paper gives an errata(that is erasure-and error-) decoding algorithm of one-point algebraic geometry codes up to the Feng-Rao designed minimum distance using Sakata's multidimensional generalization of the Berlekamp-massey algorithm and the votin procedure of Feng and Rao.......This paper gives an errata(that is erasure-and error-) decoding algorithm of one-point algebraic geometry codes up to the Feng-Rao designed minimum distance using Sakata's multidimensional generalization of the Berlekamp-massey algorithm and the votin procedure of Feng and Rao....

  11. Main: 1HU9 [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1HU9 大豆 Soybean Glycine max (L.) Merrill Seed Lipoxygenase-3 Name=Lox1.3; Synonyms=...2; CAA31664.1; ALT_SEQ.|EMBL; U50081; AAB41272.1; -.|PDB; 1HU9; X-ray; A=1-857.|PDB; 1IK3; X-ray; A=1-857.|P...WTSDTRALEAFKRFGNKLAQIENKLSERNNDEKLRNRCGPVQMPYTLLLPSSKEGLTFRGIPNSISI soybean_1HU9.jpg ...

  12. PENERAPAN FORMULA FENG SHUI DENGAN PERTIMBANGAN FORM DALAM BANGUNAN DAN LINGKUNGAN BINAAN

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    Dewi Mariana

    2015-06-01

    Full Text Available Feng Shui merupakan ilmu Tiongkok kuno yang digunakan sebagai salah satu pendekatan desain dan penataan lingkungan binaan hingga zaman ini. Namun, banyak pihak lupa, tidak sadar, atau bahkan tidak tahu bahwa Feng Shui juga mementingkan bentuk (form dan bukan hanya penerapan formula atau perhitungan saja. Masalah muncul pada image praktisi Feng Shui yang negatif, yaitu bagi mayoritas arsitek, pelaksana bangunan dan sebagian pihak pemilik proyek, jika praktisi Feng Shui terlibat, desain akan berantakan, aneh, tidak enak dilihat, dan membuat bingung mereka semua. Makalah ini mendeskripsikan dan menganalisis empat pendekatan yang lazim diterapkan oleh praktisi Feng Shui yang pada praktiknya lebih banyak menekankan penerapan formula dan melupakan bentuk (form, padahal teori dasar Feng Shui menyatakan bahwa penerapannya menitikberatkan keserasian dengan lingkungan. Di sini juga ditunjukkan bahwa masing-masing pendekatan dapat ditengahi secara logis tanpa menyalahi aturan dasar Feng Shui itu sendiri. Hasil pembahasan memperlihatkan bahwa bentuk (form dan penerapan formula dalam Feng Shui bukan merupakan hal yang saling terpisah, tetapi justru diperlukan diterapkan bersamaan untuk tujuan desain yang baik.

  13. Site Planning Here and There: Comparing Western Site Planning and Design and Eastern Feng Shui

    Directory of Open Access Journals (Sweden)

    Mintai Kim

    2004-06-01

    Full Text Available Feng Shui, in comparison with contemporary site design tools, employs a broad range of criteria in the observation and assessment of a site. Feng Shui includes information that is gathered at a different level of awareness and used to define what it is that causes a space to feel the way it does. As a result, Feng Shui may better respond to human needs and perceptions by providing the physical conditions that are comfortable for people using the space. One of the intentions of this study is to close the gap between Western ('here' and Eastern ('there' ideas. This study examines 1 whether or not Feng Shui and Western site planning are comparable, and 2 if Feng Shui can be a valid planning, design and evaluation tool for modern site planning. We examine and compare urban plazas from both Feng Shui and Western site planning perspectives. The study assessed a variety of existing urban places in the Tucson and Phoenix metropolitan areas, and Sedona, Arizona for conditions of human comfort as measured by 1 Feng Shui principles, and 2 contemporary site design recommendations. This study used the principles of Feng Shui as the criteria for evaluation of urban spaces based on human needs for physical comfort. Results of this study indicate that places that are well designed from a Feng Shui point of view are also perceived as well designed from a contemporary site design vantage. The outcome suggests that Feng Shui is a valuable tool in the site design process for Western designers.

  14. Rock Engineering Design by Xiating Feng and John A Hudson

    Institute of Scientific and Technical Information of China (English)

    E.T.Brown

    2012-01-01

    This highly original and innovative book is the outcome of the work of the Commission on Rock Engineering Design Methodology of the International Society for Rock Mechanics (ISRM) in the period 2007-2011.The work of the Commission was managed by the authors,Professor John A Hudson,ISRM President for 2007-2011,and Professor Xiating Feng,ISRM President for 2011-2015,in association with the Chinese Society for Rock Mechanics and Engineering.Recently,this reviewer (2011) has argued that the work of its Commissions has been among the major achievements of the ISRM in the 50 years since its foundation in 1962.This book adds to that impressive record of achievement.

  15. Infecciones en huéspedes inmunocomprometidos

    OpenAIRE

    Cuéllar Ponce de León, Luis Ernesto; Médico-Cirujano. Especialista en Enfermedades Infecciosas y Tropicales. Jefe del Servicio de Infectología. Instituto Nacional de Enfermedades Neoplásicas. Coordinador del Programa VIH/SIDA/TARGA-INEN. Profesor Asociado de Medicina de la Universidad Nacional Federico Villarreal. Lima.

    2013-01-01

    Las infecciones son causa frecuente de morbimortalidad en huéspedes inmunocomprometidos. La frecuencia y diversidad de infecciones en esta población dependerá del tipo de inmunocompromiso, tratamiento recibido y aspecto epidemiológico. Los huéspedes con neutropenia desarrollan infecciones por bacterias extracelulares no encapsuladas y hongos levaduriformes y filamentosos; el diagnóstico precoz y tratamiento oportuno de las mismas son considerados una emergencia por su severidad y gran mortali...

  16. An Analysis of the Feminine Awakening of Rebecca and Xi-feng

    Institute of Scientific and Technical Information of China (English)

    WANG Xue

    2013-01-01

    In Rebecca and A Dream of Red Mansions, the authors create two similar female characters -Rebecca and Wang Xi-feng,who have aroused numerous discussions among scholars. Although Rebecca and Xi-feng live in different times, they are quite similar in their characters and destinies, and are often referred as representatives of awakened female. This dissertation in-tends to conduct a cross-culture comparison between Rebecca and Xi-feng by answering the following questions: 1) what are the historical and individual causes of the feminine awakening of Rebecca and Xi-feng; 2) what are the similarities between Re-becca’s and Xi-feng’s feminine consciousness? 3) What are the differences between Rebecca and A Dream of Red Mansions in dis-playing characters’feminine consciousness?

  17. A probabilistic Hu-Washizu variational principle

    Science.gov (United States)

    Liu, W. K.; Belytschko, T.; Besterfield, G. H.

    1987-01-01

    A Probabilistic Hu-Washizu Variational Principle (PHWVP) for the Probabilistic Finite Element Method (PFEM) is presented. This formulation is developed for both linear and nonlinear elasticity. The PHWVP allows incorporation of the probabilistic distributions for the constitutive law, compatibility condition, equilibrium, domain and boundary conditions into the PFEM. Thus, a complete probabilistic analysis can be performed where all aspects of the problem are treated as random variables and/or fields. The Hu-Washizu variational formulation is available in many conventional finite element codes thereby enabling the straightforward inclusion of the probabilistic features into present codes.

  18. For Hu Zhuanglin: A Prefatory Note

    Institute of Scientific and Technical Information of China (English)

    M. A. K. Halliday

    2012-01-01

    It is a very great pleasure to be able to congratulate Professor Hu Zhuanglin on the occasion of his eightieth birthday. It is also a time to remember that Professor Hu and I have known each other for 33 years, since the moment he arrived in Sydney, together with eight other Chinese scholars, to take part in a programme of studies arranged jointly by the Department of English and the Department of Linguistics at Sydney University. These first arrivals from China were people of some seniority,

  19. Hu on poolel teel / James Miles

    Index Scriptorium Estoniae

    Miles, James

    2006-01-01

    2007. aastal toimuv kommunistliku partei 17. kongress tähistab Hiina presidendi Hu Jintao jaoks oma võimuaja poole peale jõudmist, ees ootavad sellised väljakutsed nagu olümpiamängude korraldamine, valimiskeeris Taiwanis ning hullunud majanduse stabiliseerimine. Vt samas: Hiina avanemise pidu

  20. Hu on poolel teel / James Miles

    Index Scriptorium Estoniae

    Miles, James

    2006-01-01

    2007. aastal toimuv kommunistliku partei 17. kongress tähistab Hiina presidendi Hu Jintao jaoks oma võimuaja poole peale jõudmist, ees ootavad sellised väljakutsed nagu olümpiamängude korraldamine, valimiskeeris Taiwanis ning hullunud majanduse stabiliseerimine. Vt samas: Hiina avanemise pidu

  1. Fast Erasure-and error decoding of algebraic geometry codes up to the Feng-Rao bound

    DEFF Research Database (Denmark)

    Høholdt, Tom; Jensen, Helge Elbrønd; Sakata, Shojiro

    1998-01-01

    This correspondence gives an errata (that is erasure-and error-) decoding algorithm of one-point algebraic-geometry codes up to the Feng-Rao designed minimum distance using Sakata's multidimensional generalization of the Berlekamp-Massey algorithm and the voting procedure of Feng and Rao....

  2. Feng shui väärtus on vaimujõus / Marika Tomberg ; komment. Lea Eermann, komment. Rein Weber

    Index Scriptorium Estoniae

    Tomberg, Marika

    1999-01-01

    Feng shui järgi korraldatud Lea Eermanni kodust Nõmmel. Feng shui- ja geobioloogia-alase nõustamisega tegelev arhitekt Rein Weber keskkonna mõjust tervisele, magamistoast. Enda ja maja kaitsmisest halbade mõjude eest. 10 illustratsiooni

  3. Feng shui väärtus on vaimujõus / Marika Tomberg ; komment. Lea Eermann, komment. Rein Weber

    Index Scriptorium Estoniae

    Tomberg, Marika

    1999-01-01

    Feng shui järgi korraldatud Lea Eermanni kodust Nõmmel. Feng shui- ja geobioloogia-alase nõustamisega tegelev arhitekt Rein Weber keskkonna mõjust tervisele, magamistoast. Enda ja maja kaitsmisest halbade mõjude eest. 10 illustratsiooni

  4. Hu Xiaoyu—an Outstanding Pupil

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    HU Xiaoyu, 11, is a fifth grader at the Beijing Hepingli No. 4 Primary School. She is talkative, cheerful, active and likes to laugh. Her teachers say she is well-behaved and excels in her studies. She is intelligent and always asks many questions both in and outside the classroom. She has been elected a "Three Good" student every year since she started school.

  5. Pricking, Cupping and Qu Feng Tiao Ying Decoction for Treatment of Chronic Urticaria

    Institute of Scientific and Technical Information of China (English)

    刘代红

    2002-01-01

    @@ Chronic urticaria is a commonly encountered skin illness characterized by protracted course and recurrent episodes. The author had treated 26 cases of chronic urticaria by pricking, cupping and Qu Feng Tiao Ying (QFTY) decoction (祛风调营汤the Wind-dispelling and Ying system-regulating Decoction) with satisfactory results. A report is as follows.

  6. Regulation of HuR by DNA Damage Response Kinases

    Directory of Open Access Journals (Sweden)

    Hyeon Ho Kim

    2010-01-01

    Full Text Available As many DNA-damaging conditions repress transcription, posttranscriptional processes critically influence gene expression during the genotoxic stress response. The RNA-binding protein HuR robustly influences gene expression following DNA damage. HuR function is controlled in two principal ways: (1 by mobilizing HuR from the nucleus to the cytoplasm, where it modulates the stability and translation of target mRNAs and (2 by altering its association with target mRNAs. Here, we review evidence that two main effectors of ataxia-telangiectasia-mutated/ATM- and Rad3-related (ATM/ATR, the checkpoint kinases Chk1 and Chk2, jointly influence HuR function. Chk1 affects HuR localization by phosphorylating (hence inactivating Cdk1, a kinase that phosphorylates HuR and thereby blocks HuR's cytoplasmic export. Chk2 modulates HuR binding to target mRNAs by phosphorylating HuR's RNA-recognition motifs (RRM1 and RRM2. We discuss how HuR phosphorylation by kinases including Chk1/Cdk1 and Chk2 impacts upon gene expression patterns, cell proliferation, and survival following genotoxic injury.

  7. Flexible DNA bending in HU-DNA cocrystal structures.

    Science.gov (United States)

    Swinger, Kerren K; Lemberg, Kathryn M; Zhang, Ying; Rice, Phoebe A

    2003-07-15

    HU and IHF are members of a family of prokaryotic proteins that interact with the DNA minor groove in a sequence-specific (IHF) or non-specific (HU) manner to induce and/or stabilize DNA bending. HU plays architectural roles in replication initiation, transcription regulation and site-specific recombination, and is associated with bacterial nucleoids. Cocrystal structures of Anabaena HU bound to DNA (1P71, 1P78, 1P51) reveal that while underlying proline intercalation and asymmetric charge neutralization mechanisms of DNA bending are similar for IHF and HU, HU stabilizes different DNA bend angles ( approximately 105-140 degrees ). The two bend angles within a single HU complex are not coplanar, and the resulting dihedral angle is consistent with negative supercoiling. Comparison of HU-DNA and IHF-DNA structures suggests that sharper bending is correlated with longer DNA binding sites and smaller dihedral angles. An HU-induced bend may be better modeled as a hinge, not a rigid bend. The ability to induce or stabilize varying bend angles is consistent with HU's role as an architectural cofactor in many different systems that may require differing geometries.

  8. HU-446 and HU-465, Derivatives of the Non-psychoactive Cannabinoid Cannabidiol, Decrease the Activation of Encephalitogenic T Cells.

    Science.gov (United States)

    Kozela, Ewa; Haj, Christeene; Hanuš, Lumir; Chourasia, Mukesh; Shurki, Avital; Juknat, Ana; Kaushansky, Nathali; Mechoulam, Raphael; Vogel, Zvi

    2016-01-01

    Cannabidiol (CBD), the non-psychoactive cannabinoid, has been previously shown by us to decrease peripheral inflammation and neuroinflammation in mouse experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS). Here we have studied the anti-inflammatory effects of newly synthesized derivatives of natural (-)-CBD ((-)-8,9-dihydro-7-hydroxy-CBD; HU-446) and of synthetic (+)-CBD ((+)-8,9-dihydro-7-hydroxy-CBD; HU-465) on activated myelin oligodendrocyte glycoprotein (MOG)35-55-specific mouse encephalitogenic T cells (T(MOG) ) driving EAE/MS-like pathologies. Binding assays followed by molecular modeling revealed that HU-446 has negligible affinity toward the cannabinoid CB1 and CB2 receptors while HU-465 binds to both CB1 and CB2 receptors at the high nanomolar concentrations (Ki = 76.7 ± 5.8 nm and 12.1 ± 2.3 nm, respectively). Both, HU-446 and HU-465, at 5 and 10 μm (but not at 0.1 and 1 μm), inhibited the MOG35-55-induced proliferation of autoreactive T(MOG) cells via CB1/CB2 receptor independent mechanisms. Moreover, both HU-446 and HU-465, at 5 and 10 μm, inhibited the release of IL-17, a key autoimmune cytokine, from MOG35-55-stimulated T(MOG) cells. These results suggest that HU-446 and HU-465 have anti-inflammatory potential in inflammatory and autoimmune diseases.

  9. Multiple defects in Escherichia coli mutants lacking HU protein.

    OpenAIRE

    Huisman, O; Faelen, M; Girard, D; Jaffé, A; Toussaint, A; Rouvière-Yaniv, J

    1989-01-01

    The HU protein isolated from Escherichia coli, composed of two partially homologous subunits, alpha and beta, shares some of the properties of eucaryotic histones and is a major constituent of the bacterial nucleoid. We report here the construction of double mutants totally lacking both subunits of HU protein. These mutants exhibited poor growth and a perturbation of cell division, resulting in the formation of anucleate cells. In the absence of HU, phage Mu was unable to grow, to lysogenize,...

  10. The Freudian subject and the Maoist mind: the diaries of Hermine Hug-Hellmuth and Lei Feng.

    Science.gov (United States)

    Larson, Wendy

    2011-01-01

    Although written in vastly different cultural contexts and periods, Hermine Hug-Hellmuth's "Diary of a Young Girl" (1919) and Lei Feng's "The Diary of Lei Feng" (1963) both to a large extent were written as concept pieces, with the goal of illustrating specific theories of the self. In the case of Hug-Hellmuth, the underlying theory is the Freudian sexualized unconscious mind, whereas for Lei Feng, it is Maoist revolutionary optimism. Additionally, both diaries have unusually strong inauthentic or 'fake' aspects. Although Hug-Hellmuth never admitted to writing the diary, most critics believed it came from her pen. Lei Feng's diary was revised through his own attempts to present himself as politically progressive, by editors, and through successive political movements that focused on the person behind the diary and made him one of the most widely-recognized figures in China. As such, the diaries are excellent windows into powerful and long-lasting ideological constructs.

  11. China has a natural environment, too! Consumerist and ideological eco-imaginaries in the cinema of Feng Xiaogang

    OpenAIRE

    Neri, Corrado

    2013-01-01

    International audience; Feng Xiaogang, the 'Chinese Spielberg', produces huge blockbusters and hilarious comedies directed at a broad audience. Although his work does not strictly deal with environmental issues, it provides us with a privileged perspective through which to analyse the discourses around the theme of nature. Feng Xiaogang's works are not explicitly environmental, they do address the contradictions and differing anxieties linked to China's economic and environmental development....

  12. Treatment of urticaria from Feng Xie%从风邪论治荨麻疹

    Institute of Scientific and Technical Information of China (English)

    钟卫红; 莫惠芳; 储开宇

    2011-01-01

    Feng Xie closely related with the incidence of urticaria,from exogenous and endogenous aspects of urticaria and two of "Feng Xie," produced by different ways and their clinical features, propose appropriate treatment method for an accurate clinical identify causes and provide a reference syndrome.%荨麻疹的发病同风邪有密切关系,从外感、内生两个方面探讨荨麻疹之“风邪”产生的不同途径及各自的临床特点,提出相应的治法,为临床能够准确识别病因和辨证提供参考.

  13. 阳虚生风论%Theory of Feng-Syndrome Caused by Yang Deficiency

    Institute of Scientific and Technical Information of China (English)

    潘远根

    2016-01-01

    The treatment of Feng-syndrome in TCM frequently focused on the wind syndromes caused by the strong hot, the hyperactivity of Yang, the Yin deficiency and the blood deficiency, but the Feng syndrome rarely deals with Yang deficiency. In this paper, we puts forward the Feng syndromes also caused by Yang deficiency and briefly discusses on it in the theory, the type of diseases and the syndrome differentiation, and then, to break down the syndrome differentiating keys with some clinical cases.%中医论风证的辨证治疗,向来仅注重火热、阳亢、阴虚、血虚等引起的风证,绝少论及阳虚动风。本文从阳虚生风的理论、病证类型、辨证要点等方面简要论述了阳虚阴盛同样引起动风证,并例举临床病例以详解辨证关键。

  14. A Working Day of Professor Hu Yamei

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    Professor Hu Yamei is a well-known pediatrician and one of the first group of academicians to the Institute of Medical Science attached to the Chinese Academy of Engineering. At the start of her career in the 1950s, she focused on nutritional anemia that afflicts children, developing treatment and methods of prevention. Later she researched etiology, pathology and physiology of pediatric toxic indigestion, helping to reduce the child death rate from 20 percent to 1 percent. Over the past 20 years, she has concentrated on pediatric acute lymphoblastic leukemia. As a result, the five-years living rate of the disease treated by Hu’s group has reached 74 percent, approaching the highest level in the world.

  15. Notas sobre el paisaje de Huánuco Pampa

    OpenAIRE

    Román Godines, Oscar; Ministerio de Cultura

    2013-01-01

    El complejo arqueológico Huánuco Pampa se encuentra ubicado en la provincia Dos de Mayo en el departamento de Huánuco y en la actualidad es quizás el principal vestigio de la cosmovisión y la planificación urbana inca.

  16. Rapid purification of HU protein from Halobacillus karajensis

    Directory of Open Access Journals (Sweden)

    Parinaz Ghadam

    2014-03-01

    Full Text Available The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to cruciform DNA and repair intermediates such as nick, gap, bulge, etc. To understand the features of HU binding to DNA and repair intermediates, a fast and easy HU protein purification method is required. Here we report a two-step purification procedure of HU from Halobacillus karajensis (the gram positive and moderately halophilic bacteria isolated from Karaj surface soil. The method of HU purification allows obtaining a pure non-tagged protein. Salting out and ion exchange chromatography were applied for purification, and the purified protein was identified by immunoblotting. Results showed that the molecular weight of the purified protein was approximately 11 kDa which is immunologically similar to the Bacillus subtilis HU protein (HBsu.

  17. Sparks sädeleb Rabarocki öös. Ettevaatust! Eliidi rünnak. Hu? Hu da hell?

    Index Scriptorium Estoniae

    2007-01-01

    Ameerika ansamblist Sparks. Vaiko Eplik & Eliit heliplaatidest "1: Aastaajad", "2: Aastaajad" ja "3: Kosmoseodüsseia", esinemisest festivalil Rabarock. Hannaliisa Uusma, Leslie Da Bassi ja DJ Criticali projektist HU?, singlitest "Kosmos" ja "Absoluutselt" (Rabarockil esitusel materjal HU? debüütalbumilt "Film")

  18. Sparks sädeleb Rabarocki öös. Ettevaatust! Eliidi rünnak. Hu? Hu da hell?

    Index Scriptorium Estoniae

    2007-01-01

    Ameerika ansamblist Sparks. Vaiko Eplik & Eliit heliplaatidest "1: Aastaajad", "2: Aastaajad" ja "3: Kosmoseodüsseia", esinemisest festivalil Rabarock. Hannaliisa Uusma, Leslie Da Bassi ja DJ Criticali projektist HU?, singlitest "Kosmos" ja "Absoluutselt" (Rabarockil esitusel materjal HU? debüütalbumilt "Film")

  19. 五年梦寻丰子恺--«丰子恺全集»责编手记%Searching For Feng Zikai for Five Years---Editor’s Notes for “Complete works of Feng Zikai”

    Institute of Scientific and Technical Information of China (English)

    眉睫

    2015-01-01

    近几年来,社会各界兴起了一股“丰子恺热”。海豚出版社社长俞晓群成为这股热潮的最初发动者。五年来,海豚出版社出版了丰子恺著作达一百多种,将丰子恺生前几乎所有的单行本集子予以再版。同时,海豚出版社还与杭州师范大学弘一大师丰子恺研究中心合作整理«丰子恺全集»,并将于2015年出版问世。%In recent years,the society witnessed an increasing interest in the study of Feng Zikai,in which President Yu zoqun of Dolphin became the original instigators of the craze.For five years,Dolphin Press pub-lished over one hundred kinds of books of Feng Zikai,and almost all of Feng Zikai lifetime collection booklets have been reprinted.Meanwhile,Dolphin Press have been compiling " Complete Works of Feng Zikai " in col-laboration with Normal Master Hongyi Feng Zikai Research Center,Hangzhou Normal University,which would be published in 201 5.

  20. Testing the planetary models of HU Aquarii

    CERN Document Server

    Bours, M; Breedt, E; Copperwheat, C; Dhillon, V; Leckngam, A; Littlefair, S; Parsons, S; Prasit, A

    2014-01-01

    We present new eclipse observations of the polar (i.e. semi-detached magnetic white dwarf + M-dwarf binary) HU Aqr, and mid-egress times for each eclipse, which continue to be observed increasingly early. Recent eclipses occurred more than 70 seconds earlier than the prediction from the latest model that invoked a single circumbinary planet to explain the observed orbital period variations, thereby conclusively proving this model to be incorrect. Using ULTRACAM data, we show that mid-egress times determined for simultaneous data taken at different wavelengths agree with each other. The large variations in the observed eclipse times cannot be explained by planetary models containing up to three planets, because of poor fits to the data as well as orbital instability on short time scales. The peak-to-peak amplitude of the O-C diagram of almost 140 seconds is also too great to be caused by Applegate's mechanism, movement of the accretion spot on the surface of the white dwarf, or by asynchronous rotation of the ...

  1. CB2 cannabinoid receptor agonist enantiomers HU-433 and HU-308: An inverse relationship between binding affinity and biological potency.

    Science.gov (United States)

    Smoum, Reem; Baraghithy, Saja; Chourasia, Mukesh; Breuer, Aviva; Mussai, Naama; Attar-Namdar, Malka; Kogan, Natalya M; Raphael, Bitya; Bolognini, Daniele; Cascio, Maria G; Marini, Pietro; Pertwee, Roger G; Shurki, Avital; Mechoulam, Raphael; Bab, Itai

    2015-07-14

    Activation of the CB2 receptor is apparently an endogenous protective mechanism. Thus, it restrains inflammation and protects the skeleton against age-related bone loss. However, the endogenous cannabinoids, as well as Δ(9)-tetrahydrocannabinol, the main plant psychoactive constituent, activate both cannabinoid receptors, CB1 and CB2. HU-308 was among the first synthetic, selective CB2 agonists. HU-308 is antiosteoporotic and antiinflammatory. Here we show that the HU-308 enantiomer, designated HU-433, is 3-4 orders of magnitude more potent in osteoblast proliferation and osteoclast differentiation culture systems, as well as in mouse models, for the rescue of ovariectomy-induced bone loss and ear inflammation. HU-433 retains the HU-308 specificity for CB2, as shown by its failure to bind to the CB1 cannabinoid receptor, and has no activity in CB2-deficient cells and animals. Surprisingly, the CB2 binding affinity of HU-433 in terms of [(3)H]CP55,940 displacement and its effect on [(35)S]GTPγS accumulation is substantially lower compared with HU-308. A molecular-modeling analysis suggests that HU-433 and -308 have two different binding conformations within CB2, with one of them possibly responsible for the affinity difference, involving [(35)S]GTPγS and cAMP synthesis. Hence, different ligands may have different orientations relative to the same binding site. This situation questions the usefulness of universal radioligands for comparative binding studies. Moreover, orientation-targeted ligands have promising potential for the pharmacological activation of distinct processes.

  2. On the HU Aquarii planetary system hypothesis

    Science.gov (United States)

    Goździewski, Krzysztof; Nasiroglu, Ilham; Słowikowska, Aga; Beuermann, Klaus; Kanbach, Gottfried; Gauza, Bartosz; Maciejewski, Andrzej J.; Schwarz, Robert; Schwope, Axel D.; Hinse, Tobias C.; Haghighipour, Nader; Burwitz, Vadim; Słonina, Mariusz; Rau, Arne

    2012-09-01

    In this paper, we investigate the eclipse timing of the polar binary HU Aquarii that has been observed for almost two decades. Recently, Qian et al. attributed large (O-C) deviations between the eclipse ephemeris and observations to a compact system of two massive Jovian companions. We improve the Keplerian, kinematic model of the light travel time effect and re-analyse the whole currently available data set. We add almost 60 new, yet unpublished, mostly precision light curves obtained using the time high-resolution photopolarimeter Optical Timing Analyzer (OPTIMA), as well as photometric observations performed at the Monitoring Network of Telescopes/North, Physics Innovations Robotic Astronomical Telescope Explorer and Carlos Sánchez Telescope. We determine new mid-egress times with a mean uncertainty at the level of 1 s or better. We claim that because the observations that currently exist in the literature are non-homogeneous with respect to spectral windows (ultraviolet, X-ray, visual and polarimetric mode) and the reported mid-egress measurements errors, they may introduce systematics that affect orbital fits. Indeed, we find that the published data, when taken literally, cannot be explained by any unique solution. Many qualitatively different and best-fit two-planet configurations, including self-consistent, Newtonian N-body solutions may be able to explain the data. However, using high-resolution, precision OPTIMA light curves, we find that the (O-C) deviations are best explained by the presence of a single circumbinary companion orbiting at a distance of ˜4.5 au with a small eccentricity and having ˜7 Jupiter masses. This object could be the next circumbinary planet detected from the ground, similar to the announced companions around close binaries HW Vir, NN Ser, UZ For, DP Leo, FS Aur or SZ Her, and planets of this type around Kepler-16, Kepler-34 and Kepler-35.

  3. Feng Shui in cold water production. Energy-efficient cold water unit for domestic comfort, trade and industry; Feng Shui in der Bereitstellung von Kaltwasser. Energieeffizienter Kaltwassersatz fuer Komfort, Gewerbe und Indsutrie

    Energy Technology Data Exchange (ETDEWEB)

    Roeben, J. [Menerga Apparatebau GmbH, Muelheim (Germany)

    2003-10-01

    Feng Shui means 'wind and water'. According to Chinese philosophy, all processes in nature are influenced by five different elements. This principle has always been applied in air conditioning engineering, e.g. in the development of a novel, ecological cold water production process presented by Menerga. (orig.) [German] Feng steht fuer Wind, Shui fuer Wasser. Das Zusammenspiel aller Prozesse in der Natur wird nach der chinesischen Philosophie von weiteren Grundelementen beeinflusst. In der Klimatechnik hat man sich dieses Prinzip immer wieder zunutze gemacht. So auch bei der Entwicklung eines neuen oekologischen Verfahrens zur Kaltwasserbereitstellung der Firma Menerga. (orig.)

  4. Comment on 'Investigations into the impact of astronomical phenomena on the terrestrial biosphere and climate' (arXiv:1505.07856 [astro-ph.EP]) by Fabo Feng

    CERN Document Server

    Melott, Adrian L

    2015-01-01

    This work by Feng and papers which published its conclusions do not cite nor do they deal with objections by the author published in 2013-2014. There are many fundamental problems. We will summarize here the principal problems, as published by Melott and Bambach which render irrelevant most of the work presented by Feng.

  5. Reduction in nerve root compression by the nucleus pulposus after Feng's Spinal Manipulation

    Institute of Scientific and Technical Information of China (English)

    Yu Feng; Yan Gao; Wendong Yang; Tianyou Feng

    2013-01-01

    Ninety-four patients with lumbar intervertebral disc herniation were enrolled in this study. Of these, 48 were treated with Feng's Spinal Manipulation, hot fomentation, and bed rest (treatment group). The remaining 46 patients were treated with hot fomentation and bed rest only (control group). After 3 weeks of treatment, clinical parameters including the angle of straight-leg raising, visual analogue scale pain score, and Japanese Orthopaedic Association score for low back pain were improved. The treatment group had significantly better improvement in scores than the control group. Magnetic resonance myelography three-dimensional reconstruction imaging of the vertebral canal demonstrated that filling of the compressed nerve root sleeve with cerebrospinal fluid increased significantly in the treatment group. The diameter of the nerve root sleeve was significantly larger in the treatment group than in the control group. However, the sagittal diameter index of the herniated nucleus pulposus and the angle between the nerve root sleeve and the thecal sac did not change significantly in either the treatment or control groups. The effectiveness of Feng's Spinal Manipulation for the treatment of symptoms associated with lumbar intervertebral disc herniation may be attributable to the relief of nerve root compression, without affecting the herniated nucleus pulposus or changing the morphology or position of the nerve root.

  6. A Corpus-Based Study of Chinese Classical Poem-Taking Feng Qiao Ye Bo as an Example

    Institute of Scientific and Technical Information of China (English)

    李晨曦

    2014-01-01

    In recent years,the translation of Chinese classical poem has aroused more and more attention. From the perspective of statistics, this paper will discuss the meaning of the word which the translators used. Based on the corpus linguistics, this paper will further compare and explore the translation styles of the poem Feng Qiao Ye Bo by analyzing three translators’versions.

  7. Comparing the do's & taboos in Chinese Feng-Shui and Indian Vāstu-Shāstra architectural traditions

    NARCIS (Netherlands)

    Huang, En-Yu

    2012-01-01

    Chinese Feng-Shui and Indian Vāstu-Shāstra are two of the world’s best-known and longest-enduring systems of geomancy which have, for centuries, informed architectural practice of their respective cultural spheres. Albeit developed in different geo-cultural contexts and reinforced by their distinct

  8. 宋玉《讽赋》之讽%Critics on Feng Fu by Songyu

    Institute of Scientific and Technical Information of China (English)

    刘刚

    2012-01-01

    Feng Fu written by Songyu is a piece of critics on the statement of "Chu Xiangwang is lecherous". It generalizes the rules of female' s showing of love and basic principles of male' s self-control, which provides references for monarchs to identify woman' s charm. The nature of it is to prevent obscene.%宋玉的《讽赋》是针对“楚襄王好女色”的专题讽谏文章,全文借自我辩白,概括了女性示爱的典型规律和男性自律的基本规范,为君王认知女色和应对女色提供了经验性借鉴,其讽谏的实质是止欲与戒淫。

  9. Gauge theory one-loop amplitudes and the Britto-Cachazo-Feng-Witten recursion relations

    Science.gov (United States)

    Kharel, Savan; Siopsis, George

    2012-07-01

    We calculate gauge theory one-loop amplitudes with the aid of the complex shift used in the Britto-Cachazo-Feng-Witten (BCFW) recursion relations of tree amplitudes. We apply the shift to the integrand and show that the contribution from the limit of infinite shift vanishes after integrating over the loop momentum, with a judicious choice of basis for polarization vectors. This enables us to write the one-loop amplitude in terms of on shell tree and lower-point one-loop amplitudes. Some of the tree amplitudes are forward amplitudes. We show that their potential singularities do not contribute and the BCFW recursion relations can be applied in such a way as to avoid these singularities altogether. We calculate in detail n-point one-loop amplitudes for n=2, 3, 4, and outline the generalization of our method to n>4.

  10. L’EVOLUTION HYDRODYNAMIQUE DES AFTERGLOWS POUR LE MODELE DE FENG

    Directory of Open Access Journals (Sweden)

    E ZOUAOUI

    2015-06-01

    Full Text Available Nous avons modélisé l'évolution du choc externe produit par la décélération d'un jet relativiste, éjecté par un progéniteur d'un sursaut gamma, par un milieu interstellaire (MIS. Nous avons adopté le modèle de Feng et al. (2002, étant donné qu'il est le modèle le plus réaliste dans la description de l'énergie interne produite par le choc et celle restituée dans la boule de feu, dans un autre côté son accord avec les solutions de Sedov avec ses conditions.

  11. 冯梦龙的小说理论%Feng Menglong' s Novel Theory

    Institute of Scientific and Technical Information of China (English)

    傅承洲

    2012-01-01

    Feng Menglong composed three prefaces for his own novels San Yah. Though each preface focused on one question, they connected closely with and complemented each other, forming Feng's own novel theoretical system. The Prefaces of Ancient and Contemporary Novels first proposed the Popular Romance was originated in the art of speaking, compared to the Tang Dynasty folktales, the Popular Romance language to content are easy for or- dinary people to understand, and to accept and appreciate, playing a greater role in the society. The Preface of Jingshitongyaa explores the relationship between real life and artistic fiction, Feng used the fictional method that the figure does not have to experience such events, and such events do not have to happen to the figure. " Fiction should be composed to the rule that when event is real, the reason should not be pseudo; while the event is pseudo, the reason should also be. true. The story of the novel may be fictional, but historical novel should be based on strict historical facts. The "Preface of Xingshihengyan put forward the "theory of awakening the world," Feng Menglong bitterly felt the turbid and chaos of the late Ming Dynasty, he wanted to use the Popular Romance to alert the world to turn things around. To achieve the purpose of awakening the world, the popular fiction should be created with a good content, but also have to follow the laws of the novel.%冯梦龙为“三言”所作的三篇序,每篇重点论述一个问题,各篇又相互勾连补充,形成了自己的小说理论体系。《古今小说叙》首次提出通俗演义起源于说话艺术,与唐人传奇相比,通俗演义从语言到内容都通俗易懂,可以为普通百姓所接受和欣赏,发挥更大的社会作用。《警世通言叙》探讨了小说创作中生活真实与艺术虚构的关系问题,提出了“入不必有其事,事不必丽其人”的虚构方法,虚构要做到“事真而理不赝,即事赝而理亦

  12. Hu Shi's Study of Chinese Medieval intellectual History

    Institute of Scientific and Technical Information of China (English)

    Lou Yulie

    2006-01-01

    Hu Shi frequently gave lectures on the history of Chinese philosophy,especially the history of ancient Chinese philosophy,from the year 1919 to 1937.A large number of papers and dissertations published during this period are related to his research on this topic.In his opinion,there are three characteristics of the history of ancient Chinese philosophy:" religionalization of thought," "Indianization of philosophy," and "conflict between Chinese thought and Indian thought." In this paper,I explore Hu Shi's deep insight into the religionalization of Confucianism in Han dynasty and into the thought of Taoism in the medieval times.

  13. Treatment of β-Thalassemia With Hydroxyurea (HU)——Effects of HU on Globin Gene Expression

    Institute of Scientific and Technical Information of China (English)

    黄淑帧; 任兆瑞; 陈美珏; 许洪平; 曾溢滔; G.P.Rodgers; 曾凡一; A.N.Schechter

    1994-01-01

    A newly developed method of RT-PCR/competitive PCR for measuring the relative and ab-solute content of globin mRNAs as well as micro-globin chain biosynthetic assay have been used to study thealterations of globin gene expressions in the patients with β-thalassemia pre-and post-hydroxyurea(HU)treatment.It was found for the first time that HU had the effect of enhancing β-globin gene expression insome patients.Two cases with β-thalassemia who were subjected to HU treatment for over two years showeda marked increase in β-globin mRNA level and β-globin chain synthesis,resulting in more effective erythro-poiesis and the alleviation of clinical symptoms.

  14. HU-444, a Novel, Potent Anti-Inflammatory, Nonpsychotropic Cannabinoid.

    Science.gov (United States)

    Haj, Christeene G; Sumariwalla, Percy F; Hanuš, Lumír; Kogan, Natalya M; Yektin, Zhana; Mechoulam, Raphael; Feldmann, Mark; Gallily, Ruth

    2015-10-01

    Cannabidiol (CBD) is a component of cannabis, which does not cause the typical marijuana-type effects, but has a high potential for use in several therapeutic areas. In contrast to Δ(9)-tetrahydrocannabinol (Δ(9)-THC), it binds very weakly to the CB1 and CB2 cannabinoid receptors. It has potent activity in both in vitro and in vivo anti-inflammatory assays. Thus, it lowers the formation of tumor necrosis factor (TNF)-α, a proinflammatory cytokine, and was found to be an oral antiarthritic therapeutic in murine collagen-induced arthritis in vivo. However, in acidic media, it can cyclize to the psychoactive Δ(9)-THC. We report the synthesis of a novel CBD derivative, HU-444, which cannot be converted by acid cyclization into a Δ(9)-THC-like compound. In vitro HU-444 had anti-inflammatory activity (decrease of reactive oxygen intermediates and inhibition of TNF-α production by macrophages); in vivo it led to suppression of production of TNF-α and amelioration of liver damage as well as lowering of mouse collagen-induced arthritis. HU-444 did not cause Δ(9)-THC-like effects in mice. We believe that HU-444 represents a potential novel drug for rheumatoid arthritis and other inflammatory diseases.

  15. HuR in pheochromocytomas and paragangliomas - overexpression in verified malignant tumors.

    Science.gov (United States)

    Leijon, Helena; Salmenkivi, Kaisa; Heiskanen, Ilkka; Hagström, Jaana; Louhimo, Johanna; Heikkilä, Päivi; Ristimäki, Ari; Paavonen, Timo; Metso, Saara; Mäenpää, Hanna; Haglund, Caj; Arola, Johanna

    2016-09-01

    Pheochromocytomas and paragangliomas are rare, neural crest-originating, neuroendocrine tumors. HuR is an mRNA-binding protein of the ELAV/Hu-protein family, which participates in posttranscriptional regulation of many cancer-associated genes. HuR expression has been connected with aggressive behavior of several malignancies. Cyclooxygenase-2 (COX-2) is also expressed in several malignant tumors, and its expression is regulated by HuR. Tissue microarray of 153 primary pheochromocytomas and paragangliomas was investigated for the expression of HuR and COX-2 proteins by immunohistochemistry using two different HuR antibodies (HuR19F12 and HuR3A). In these tumors, the expression of both intranuclear and cytoplasmic HuR was detectable. Increased cytoplasmic HuR expression was significantly associated with metastatic tumors. Increased COX-2 and MIB-1 expression also was associated with metastatic potential, and moreover, HuR and COX-2 expression correlated with each other. Our data suggest that increased expression of HuR protein is associated with metastatic potential of paragangliomas and pheochromocytomas, and COX-2 seems to be a target of HuR.

  16. Structure-based analysis of HU-DNA binding.

    Science.gov (United States)

    Swinger, Kerren K; Rice, Phoebe A

    2007-01-26

    HU and IHF are prokaryotic proteins that induce very large bends in DNA. They are present in high concentrations in the bacterial nucleoid and aid in chromosomal compaction. They also function as regulatory cofactors in many processes, such as site-specific recombination and the initiation of replication and transcription. HU and IHF have become paradigms for understanding DNA bending and indirect readout of sequence. While IHF shows significant sequence specificity, HU binds preferentially to certain damaged or distorted DNAs. However, none of the structurally diverse HU substrates previously studied in vitro is identical with the distorted substrates in the recently published Anabaena HU(AHU)-DNA cocrystal structures. Here, we report binding affinities for AHU and the DNA in the cocrystal structures. The binding free energies for formation of these AHU-DNA complexes range from approximately 10-14.5 kcal/mol, representing K(d) values in the nanomolar to low picomolar range, and a maximum stabilization of at least approximately 6.3 kcal/mol relative to complexes with undistorted, non-specific DNA. We investigated IHF binding and found that appropriate structural distortions can greatly enhance its affinity. On the basis of the coupling of structural and relevant binding data, we estimate the amount of conformational strain in an IHF-mediated DNA kink that is relieved by a nick (at least 0.76 kcal/mol) and pinpoint the location of the strain. We show that AHU has a sequence preference for an A+T-rich region in the center of its DNA-binding site, correlating with an unusually narrow minor groove. This is similar to sequence preferences shown by the eukaryotic nucleosome.

  17. Structure-based Analysis to Hu-DNA Binding

    Energy Technology Data Exchange (ETDEWEB)

    Swinger,K.; Rice, P.

    2007-01-01

    HU and IHF are prokaryotic proteins that induce very large bends in DNA. They are present in high concentrations in the bacterial nucleoid and aid in chromosomal compaction. They also function as regulatory cofactors in many processes, such as site-specific recombination and the initiation of replication and transcription. HU and IHF have become paradigms for understanding DNA bending and indirect readout of sequence. While IHF shows significant sequence specificity, HU binds preferentially to certain damaged or distorted DNAs. However, none of the structurally diverse HU substrates previously studied in vitro is identical with the distorted substrates in the recently published Anabaena HU(AHU)-DNA cocrystal structures. Here, we report binding affinities for AHU and the DNA in the cocrystal structures. The binding free energies for formation of these AHU-DNA complexes range from 10-14.5 kcal/mol, representing K{sub d} values in the nanomolar to low picomolar range, and a maximum stabilization of at least 6.3 kcal/mol relative to complexes with undistorted, non-specific DNA. We investigated IHF binding and found that appropriate structural distortions can greatly enhance its affinity. On the basis of the coupling of structural and relevant binding data, we estimate the amount of conformational strain in an IHF-mediated DNA kink that is relieved by a nick (at least 0.76 kcal/mol) and pinpoint the location of the strain. We show that AHU has a sequence preference for an A+T-rich region in the center of its DNA-binding site, correlating with an unusually narrow minor groove. This is similar to sequence preferences shown by the eukaryotic nucleosome.

  18. HU-GFP and DAPI co-localize on the Escherichia coli nucleoid.

    Science.gov (United States)

    Wery, M; Woldringh, C L; Rouviere-Yaniv, J

    2001-02-01

    The heterodimeric HU protein, one of the most abundant DNA binding proteins, plays a pleiotropic role in bacteria. Among others, HU was shown to contribute to the maintenance of DNA superhelical density in Escherichia coli. By its properties HU shares some traits with histones and HMG proteins. More recently, its specific binding to DNA recombination and repair intermediates suggests that HU should be considered as a DNA damage sensor. For all these reasons, it will be of interest to follow the localization of HU within the living bacterial cells. To this end, we constructed HU-GFP fusion proteins and compared by microscopy the GFP green fluorescence with images of the nucleoid after DAPI staining. We show that DAPI and HU-GFP colocalize on the E. coli nucleoid. HU, therefore, can be considered as a natural tracer of DNA in the living bacterial cell.

  19. Targeting Mycobacterium tuberculosis nucleoid-associated protein HU with structure-based inhibitors

    Science.gov (United States)

    Bhowmick, Tuhin; Ghosh, Soumitra; Dixit, Karuna; Ganesan, Varsha; Ramagopal, Udupi A.; Dey, Debayan; Sarma, Siddhartha P.; Ramakumar, Suryanarayanarao; Nagaraja, Valakunja

    2014-06-01

    The nucleoid-associated protein HU plays an important role in maintenance of chromosomal architecture and in global regulation of DNA transactions in bacteria. Although HU is essential for growth in Mycobacterium tuberculosis (Mtb), there have been no reported attempts to perturb HU function with small molecules. Here we report the crystal structure of the N-terminal domain of HU from Mtb. We identify a core region within the HU-DNA interface that can be targeted using stilbene derivatives. These small molecules specifically inhibit HU-DNA binding, disrupt nucleoid architecture and reduce Mtb growth. The stilbene inhibitors induce gene expression changes in Mtb that resemble those induced by HU deficiency. Our results indicate that HU is a potential target for the development of therapies against tuberculosis.

  20. 冯玉祥集团与晋绥地区%Feng Yuxiang Group and Jin-Sui Region

    Institute of Scientific and Technical Information of China (English)

    贾熟村

    2012-01-01

    Feng Yuxiang advocated Sun Yat-sen's revolutionary policy of unitig Rusia,uniting the communist party and supporting farmers and workers, while Yah Xishan was basically against it. The relationship between them changed constantly, separating and uniting many times. After the surrender of Japan, Yan followed Jiang Kai-shek and started the civil war. He did not accept Feng Yuxiang's persvasion and ended up in failure.%冯玉祥奉行孙中山联俄、联共、扶助农工的革命政策,阎锡山基本上是反对三大政策的,双方分分合合,变幻多端。日本投降后,阎锡山追随蒋介石,挑起内战,不听冯玉祥的忠告,终归于失败。

  1. 试论胡风文学主体性内涵%On the Connotations of Hu Feng’s Literature Subjectivity

    Institute of Scientific and Technical Information of China (English)

    吴亚南

    2012-01-01

      胡风是中国第一个真正实质意义上建立了完整的以马克思主义思想为基础的主体性现实主义理论体系的文艺思想家。他结合时代历史语境和自身艺术实践,提出了“主观战斗精神”等一系列强调文艺主体性作用的文学主张,丰富了中国马克思主义文学批评的理论内涵,有效拓宽了马克思主义文学批评的知识视野和理论空间。论文分别从主体性思考的知识逻辑起点、实践主体性视野、历史条件下的复合主体性内涵三个方面探讨胡风对中国马克思主义文学批评理论建设的贡献。%  Hu Feng is the first Chinese critic who truly establishes subjective realism literature theory based on Marxist literary criticism. Combining historical context of times and his own artistic practice, he advo⁃cates subjective fighting spirit and series of literary claims which emphasize literary subjective importance. His literary claims enrich the connotations of Chinese Marxist literary criticism. and also effectively broaden the vi⁃sion and theoretical space. This paper mainly expounds about Hu Feng’s contribution from three aspects to the construction of Chinese Marxist literary criticism:the knowledge and logic starting point of subjective thinking, practical vision of subjectivity, the compound subjectivities of historical conditions.

  2. The Difference in the Main Conflict between Shakespeare ’s Hamlet and Feng Xiaogang’s The Banquet

    Institute of Scientific and Technical Information of China (English)

    江瑜婷

    2014-01-01

    Famous Chinese director Feng Xiaogang made a film The Banquet in 2006, which is adapted from Shakespeare’s play Hamlet. However, accounting for the background and cultural difference, the main conflict shown in this film is totally different from Hamlet. The paper will compare Hamlet’s reaction to his father’s death with the plot of the film to illustrate the difference of main conflict between Hamlet and The Banquet.

  3. Feng Youlan’s Interpretation of Western Philosophy:A Critical Examination from the Perspective of Metaphysical Methodology

    Directory of Open Access Journals (Sweden)

    Derong Chen

    2015-04-01

    Full Text Available The paper aims to display a limited observation of Feng’s interpretation of Western philosophy through the window of metaphysical methodology. This paper concentrates on Feng’s interpretation of Western philosophy from the perspective of metaphysical methodology and aims to display a limited observation of Feng’s interpretation of Western philosophy through the window of metaphysical methodology. Based on a brief review the recent studies of Feng Youlan and Western philosophy, this paper analyzes the progress and insufficient aspects in current studies on this issue and particularly clarifies what are the metaphysics and metaphysical methods in the context of Feng Youlan’s philosophy. In clarifying Feng’s interpretation of Western philosophy from the perspective of methodology, this paper further critically analyzes the Feng’s positive metaphysical methods and negative metaphysical methods, and assumes that Feng’s negative metaphysical methods essentially is a kind of attitudes towards metaphysics but neither a kind of metaphysics nor a kind of metaphysical methods. Instead of characterizing metaphysical methods as positive and negative as Feng did, this paper suggests an alternative division of metaphysical methods: direct and indirect methods of dealing with metaphysical issues.

  4. Progress in technology for the 2005 height determination of Qomolangma Feng (Mt. Everest)

    Institute of Scientific and Technical Information of China (English)

    CHEN; Junyong; YUAN; Janli; GUO; Chunxi; ZHANG; Yanping; ZHANG; Peng

    2006-01-01

    In 2005 China carried out a new geodetic campaign for the height determination of Qomolangma Feng--Mt. Everest (QF in short). The technical progresses in geodesy for the 2005 campaign are presented in the paper. GPS positioning was the key technique in the campaign. After summarizing the experiences and lessons of the GPS positioning on the QF summit in the previous QF height determination campaigns, some measures were taken to raise the accuracy and reliability of the height determination with GPS techniques. In order to raise the accuracy of the height determination of the QF summit with classical geodetic techniques, laser ranging was used together with the trigonometric levelling in the 2005 campaign. It is the first time in China the thickness of the ice-snow layer on the QF summit was measured by ground penetrating radar integrated with GPS. The local gravity field and geoid in the QF area was improved on the basis of earth gravity field model integrated with new ground gravity data, DTM data and GPS leveling data in the QF area. In the 2005 campaign the normal height and orthometric height (height above sea level) of the snow surface of the QF summit were obtained as 8846.67 m and 8847.93 m respectively. The orthometric height of the rock surface of the QF summit is 8844.43 m,and the thickness of the ice-snow layer on the QF summit is 3.50 m.

  5. Study on the volcanic ash cloud with Feng Yun-3 meteorological satellite data

    Science.gov (United States)

    Gong, Cai-lan T.; Jiang, Shan; Hu, Yong; Meng, Peng

    2013-09-01

    Volcano eruption can produce a mass of volcanic ash floating in the air for a long period, which will seriously threaten the aerial planes safety, and cause the air pollution, it could do harm to people's living environment and their health. Take the Iceland Eyjafjallajokull volcano as an example which erupted in April to May 2010, the volcano ash cloud were derived with the visible and infrared scanning radiometer of FengYun-3(FY-3 VIRR) meteorological satellite data. The medium wave infrared (MWIR) and the thermal infrared split windows (THIR-SW) data were used separately. the MODIS THIR-SW data were also be used to retrieve ash cloud to test the results derived from FY-3 VIRR data. It showed that the MWIR was more applicable for the ash cloud retrieving than the THIR-SW with FY-3 VIRR data, and the threshold value should be adjusted to around negative 1 rather than 0 for VIRR THIR-SW data. And the threshold should be adjusted with the THIR-SW of FY-3. The ash cloud radiation and bright temperature(BT), spatial distribution characteristics were also analyzed quantitatively with the two channels data. The study could provide parameters for the prediction of volcanic ash cloud dispersion simulate. When the real temperature of lava flow were high enough, the sensor will show a false bright temperature, how to retrieve the real temperature of the higher lava flow is a problem need to be studied in the future.

  6. Genesis Research与Morphosys扩展HuCAL抗体协议

    Institute of Scientific and Technical Information of China (English)

    黄晓燕(摘)

    2008-01-01

    Genesis Research与Morphosys扩展协议后,靶向FGFR5蛋白的抗体将继续作为其研究的焦点。Genesis将利用Morphosys分公司Serotec开发的人组织抗体库(HuCAL)继续开发成纤维细胞生长因子受体5(FGFR5)靶点。

  7. 风水研究现状反思(上)%Reflections on the Current Study of Feng Shui

    Institute of Scientific and Technical Information of China (English)

    胡义成

    2015-01-01

    中国风水在哲学和科学上均大异于西方,“五四”后却被判为“迷信”。改革开放后,天津大学建筑学教授王其亨先生率先再审风水,认为风水虽杂有迷信成分,但它实际是中国古代地理学、气象学、生态学、景观学、规划学、建筑学等自然科学群集,应加以批判继承,引起“风水热”,也激起反弹。在此后国内学界关于风水“含科学说”和风水“迷信说”的争论中,虽然风水“意匠说”和“美学说”获某种共识,但风水“含科学说”至今面对严重挑战。目前,作为风水“孪生学术”的中医“含科学说”越来越获公认,国内风水研究亟待“突围”。由于吴良镛等人认同王其亨思路,目前这种突破正在实施。%The Chinese Feng Shui in philosophy and science has great different to that of the west , but since the May 4th Movement, it has been judged as “superstition.” After the reform and open-up, the Architecture Pro-fessor Wang Qiheng with Tianjin University first reexamined Feng Shui, he believes though there is a superstition ingredient in Feng Shui, it actually is the ancient Chinese cluster of geography , meteorology, ecology, landscape, planning , architecture , and other natural science , and it should be critically inherited .This has made Feng Shui a hot issue .Later, the hot debate in the domestic academic circles about “science-included theory” and “supersti-tion theory” has won some consensus for both the “artistic conception” and“aesthetic” of Feng Shui, but today the“science-included theory” still faces serious challenges .At present , the“science-included theory” of the tradition-al Chinese medicine , as the twin brother of Feng Shui, has got much recognition , so the domestic research of Feng Shui needs a urgent break-through .Because Wu Liangyong and others agree with Wang Qiheng ideas , such a breakthrough is being implemented .

  8. Active cooling convectors in the ''Altstadt-Palais'' building. Feng Shui criteria and users' wishes were considered; Aktive Kuehlkonvektoren im Altstadt-Palais. Feng Shui-Kriterien und Nutzerwuensche beruecksichtigen

    Energy Technology Data Exchange (ETDEWEB)

    Schmid, Wolfgang

    2009-07-01

    Modern air conditioning systems are not chosen for technical and economic aspects alone, but there are various boundary conditions that are generally considered. In the case of the Altstadt-Palais building at Karl-Scharnagl-Ring, Munich, which has an office floor space of about 9,800 m{sup 2}, also Feng Shui criteria were taken into account apart from 'normal' criteria like room flexibility, thermal comfort, and economic efficiency. The new system has cooling convectors with primary air supply that are integrated in the ceilings. (orig.)

  9. The New Chinese Cultural Landscape Feng Shui Applications%新中式景观中的风水文化演绎

    Institute of Scientific and Technical Information of China (English)

    施雪燕; 武文婷

    2016-01-01

    New Chinese landscape is a modern interpretation of traditional Chinese culture, and Feng Shui culture is unique traditional culture. Based on previous studies on Feng Shui culture, explore new polyol derived Chinese culture Feng Shui landscape design and interpretation of the new Chinese landscape Feng Shui culture, from different angles of the new Chinese cultural landscape Feng Shui use to think.%新中式景观是对中国传统文化的现代性演绎,而风水文化是中国独特的传统文化。文章在前人风水文化研究的基础上,探讨了新中式景观中风水文化的多元衍化以及新中式景观中风水文化的设计演绎,从不同的角度对新中式景观中的风水文化运用进行思考。

  10. Concept "feng shui" as a word of Chinese origin in the consciousness of native Russian language (based on the psycholinguistic experiments

    Directory of Open Access Journals (Sweden)

    Yang Xi

    2016-12-01

    Full Text Available This article studies one borrowing from Chinese – «feng shui» with the standpoint of cognitive science and psycholinguistics. According to the statistics from «The Russian National Corpus», «YandexNews» and our experiments we can conclude that this borrowing «feng shui» is quite common in Russian language. We expand research based on the theory and method of Russian linguists: Z.D. Popova and I.A. Sternin, implying three steps for description of concept «feng shui»: 1 describe macrostructure of concept; 2 describe categorical structure of concept; 3 describe field organization of concept. According to the data of psycholinguistic experiments we can point out not only the adaptive information of «feng shui» among Russianspeakers, but also concept content. Associative experiments create a concept model of «feng shui», and divide it into the core, proximal, distal and the extreme periphery.

  11. HuCOP1 contributes to the regulation of DNA repair in keratinocytes.

    Science.gov (United States)

    Fazekas, B; Carty, M P; Németh, I; Kemény, L; Széll, M; Ádám, É

    2017-03-01

    We have previously demonstrated that the E3 ligase Human Constitutive Photomorphogenic Protein (huCOP1) is expressed in human keratinocytes and negatively regulates p53. The MutS homolog 2 (MSH2) protein plays a central role in DNA MMR mechanism and is implicated in the cellular response to anticancer agents, such as cisplatin. Our aim was to clarify whether huCOP1 plays a role in DNA MMR by affecting MSH2 protein level in human keratinocytes. To define the role of huCOP1 in DNA mismatch repair, we determined whether huCOP1 affects MSH2 abundance. MSH2 protein level was detected by immunocytochemical staining using a keratinocyte cell line in which the expression level of huCOP1 was stably decreased (siCOP1). To investigate whether huCOP1 silencing influences cisplatin-induced cell death, control and siCOP1 keratinocyte cells were treated with increasing concentrations of cisplatin and cell viability was recorded after 48 and 96 h. Stable silencing of huCOP1 in human keratinocytes resulted in a reduced level of MSH2 protein. huCOP1 silencing also sensitized keratinocytes to the interstrand crosslinking inducer cisplatin. Our results indicate that decreased huCOP1 correlates with lower MSH2 levels. These protein level changes lead to increased sensitivity toward cisplatin treatment, implicating that huCOP1 plays a positive role in maintaining genome integrity in human keratinocytes.

  12. Low level anti-Hu reactivity: a risk marker for small cell lung cancer?

    Science.gov (United States)

    Tsou, Jeffrey A.; Kazarian, Meleeneh; Patel, Ankur; Galler, Janice S.; Laird-Offringa, Ite A.; Carpenter, Catherine L.; London, Stephanie J.

    2010-01-01

    Background Previous experimental and laboratory studies have implicated antibodies against Hu proteins (anti-Hu) as a potential marker for small cell lung cancer (SCLC); there are no estimates of the association between anti-Hu and SCLC using a population-based design. Methods We used stored plasma specimens to evaluate anti-Hu reactivity in relationship to small cell lung cancer in a population-based case-control study. Using Western Blot analysis, we measured anti-Hu reactivity against recombinant Hu family member, HuD, in plasma samples from forty-one (41) SCLC cases and seventy-nine (79) controls individually matched for age, race, sex, and smoking status (never, past, current). We analyzed the association between anti-Hu reactivity and SCLC using conditional logistic regression. Results Anti-Hu reactivity was associated with SCLC, both before and after adjustment for amount of smoking. We observed a smoking-adjusted odds ratio of 3.2 (95 % confidence interval from 0.98 to 13.4) comparing subjects above 1800 units (the lower limit of the second tertile of the distribution among antibody positive controls) to subjects with lower reactivity. We also found suggestive evidence in follow-up of our cases that anti-Hu above 1800 units was related to longer-term survival from SCLC. The present research is the first report of anti-Hu reactivity and SCLC in a population-based study. Conclusions Given the suggestive evidence in this study, prospective analyses to examine whether anti-Hu reactivity might predict risk of developing SCLC, or whether anti-Hu reactivity could serve as an early marker for SCLC, may be warranted. PMID:19070439

  13. 冯梦龙的音乐文学思想%Thoughts Expressed in Feng Menglong's Musical Literature

    Institute of Scientific and Technical Information of China (English)

    王慧

    2011-01-01

    Feng Menglong, an eminent litterateur in the Ming Dynasty, obtaines remarkable achievements in literature. However, people oftentimes merely focus on the novels edited and selected by Feng Menglong, while just superficially studying his other works. Mountain Songs and Taixia Xinzou, anthologies of folk songs of that time collected by Feng on his own, provide helpful materials for the study on vulgar literature development and local folk customs. Several parts of the collections are indicators of Feng' s insights into such musical literature as folk songs and vulgar music, which constitute an essential part of Chinese ancient musical and literary theories.%明代杰出文学家冯梦龙在文学领域的成就颇丰,然而世人对他的关注往往只停留在他编选的小说上,对其他方面的研究相对不够深入。他凭借个人之力搜集、编辑了当时的民歌俗曲集《山歌》、《太霞新奏》等作品,为后世研究当时俗文学和地方的民俗风情提供了宝贵的资料,其中某些篇章道出了冯梦龙对民歌俗曲这类音乐文学的独特见解,成为我国古代乐论、文论的重要组成部分。

  14. 试析丰子恺的人生论美学观%On Feng Zikai’s Life Aesthetics

    Institute of Scientific and Technical Information of China (English)

    余连祥

    2015-01-01

    Feng Zikai thinks that life is depressed and the way to escape from depression is art and religion. From a practical point of view, art is useless. However, Feng advocates life with art and art with life and believes that art can beautify life, and“comfort our feelings with a subtle way”. This is the“big use”of art. Feng Zikai claimed to be an admirer of children and that“children heart”, art heart and religion heart are in harmoniously combination. Art can nurture“children heart”and maintain art heart and religion heart of the adult.%丰子恺认为,人生是苦闷的,脱离苦海的途径便是艺术和宗教。从实用的角度看,艺术是无用的。然而,丰子恺主张艺术的人生化和人生的艺术化,认为艺术能美化人生,且能“用慰安的方式来潜移默化我们的感情”,这是艺术的“大用”。丰子恺自称是儿童崇拜者,认为“童心”与艺术心、宗教心、“赤子之心”三位一体,艺术能长养“童心”,永葆成人的艺术心、宗教心和“赤子之心”。

  15. IHF and HU: flexible architects of bent DNA.

    Science.gov (United States)

    Swinger, Kerren K; Rice, Phoebe A

    2004-02-01

    The energetic cost of bending short segments of DNA is very high. This bending is critical for the packaging of DNA and is exploited to regulate many cellular processes. In prokaryotes, IHF and HU are key architectural proteins present at high concentrations. New protein-DNA co-crystal structures, and the adaptation of advanced biophysical and biochemical techniques have led to an improved understanding of how these proteins interact with DNA. These techniques include time-resolved synchrotron X-ray footprinting, differential scanning calorimetry, isothermal titration calorimetry and single-molecule experiments.

  16. Influence of surface-normal ground acceleration on the initiation of the Jih-Feng-Erh-Shan landslide during the 1999 Chi-Chi, Taiwan, earthquake

    Science.gov (United States)

    Huang, C.-C.; Lee, Y.-H.; Liu, Huaibao P.; Keefer, D.K.; Jibson, R.W.

    2001-01-01

    The 1999 Chi-Chi, Taiwan, earthquake triggered numerous landslides throughout a large area in the Central Range, to the east, southeast, and south of the fault rupture. Among them are two large rock avalanches, at Tsaoling and at Jih-Feng-Erh-Shan. At Jih-Feng-Erh-Shan, the entire thickness (30-50 m) of the Miocene Changhukeng Shale over an area of 1 km2 slid down its bedding plane for a distance of about 1 km. Initial movement of the landslide was nearly purely translational. We investigate the effect of surface-normal acceleration on the initiation of the Jih-Feng-Erh-Shan landslide using a block slide model. We show that this acceleration, currently not considered by dynamic slope-stability analysis methods, significantly influences the initiation of the landslide.

  17. Un ente innovativo di ricerca e di servizi per la informazione geospaziale: GIS Research Center della Feng-Chia University di Taiwan (GIS.FCU

    Directory of Open Access Journals (Sweden)

    Mauro Salvemini

    2012-04-01

    Full Text Available La storia di un Laboratorio GIS a Taiwan nato da un accordo tra Feng-Chia University e la Università di Roma “LaSapienza” oggi tra i primi nel mondo nel settore dell’informazione geospaziale per la ricerca nell’ambito dei testdell’interoperabilità.A body of research and innovative services for geospatial infor-mation: GIS Research Center of Feng-Chia University in Taiwan (GIS.FCUThe story of a GIS laboratory in Taiwan born on an agreement between the Feng-Chia University and the University of Rome "La Sapienza" now among the first in the world in the field of information for research for geospatial application.

  18. HuPSON: the human physiology simulation ontology.

    Science.gov (United States)

    Gündel, Michaela; Younesi, Erfan; Malhotra, Ashutosh; Wang, Jiali; Li, Hui; Zhang, Bijun; de Bono, Bernard; Mevissen, Heinz-Theodor; Hofmann-Apitius, Martin

    2013-11-22

    Large biomedical simulation initiatives, such as the Virtual Physiological Human (VPH), are substantially dependent on controlled vocabularies to facilitate the exchange of information, of data and of models. Hindering these initiatives is a lack of a comprehensive ontology that covers the essential concepts of the simulation domain. We propose a first version of a newly constructed ontology, HuPSON, as a basis for shared semantics and interoperability of simulations, of models, of algorithms and of other resources in this domain. The ontology is based on the Basic Formal Ontology, and adheres to the MIREOT principles; the constructed ontology has been evaluated via structural features, competency questions and use case scenarios.The ontology is freely available at: http://www.scai.fraunhofer.de/en/business-research-areas/bioinformatics/downloads.html (owl files) and http://bishop.scai.fraunhofer.de/scaiview/ (browser). HuPSON provides a framework for a) annotating simulation experiments, b) retrieving relevant information that are required for modelling, c) enabling interoperability of algorithmic approaches used in biomedical simulation, d) comparing simulation results and e) linking knowledge-based approaches to simulation-based approaches. It is meant to foster a more rapid uptake of semantic technologies in the modelling and simulation domain, with particular focus on the VPH domain.

  19. Effect of Direct Sowing Rates on Mid-season “Rice Feng-liang-you-xiang-1”

    Directory of Open Access Journals (Sweden)

    Wen-jing He

    2013-04-01

    Full Text Available To study the suitable seeding rate of middle-season rice sown directly in Jianghan Plain, 6 treatments were set up with different seeding rates for hybrid rice Feng-liang-you-xiang-1 which was sown directly: 22.5, 30, 37.5, 45, 52.5, 60 kg/hm2, respectively and the optimum seeding rate was selected. Some initiative results were made. The seeding rate influences whole growth period, yield components, yield and relative economic output of Feng-liang-you-xiang-1 significantly. It shows a quadratic curve relationship between seeding rate and whole growth period and when the seeding rates were less than 30 kg/hm2 or more than 52.5 kg/hm2, the whole growth period was obviously shortened compared with that of other seeding rate. Among the yield components, panicles, grains panicles, seed-setting rate and harvest index are effected very significantly by the seeding rate, showing a linear relationship of quadratic curve; while to 1000 seed weight the influence don’t reach the level of significant difference. And seeding rate shows in curve relation to yield (y = 2.5248x2 + 198.89x + 7620.1; when the seeding rate is 39.387 kg/hm2, the yield reaches the maximum value of 11536.95 kg/hm2.

  20. SUPPORT FOR HU CFRT SUMMER HIGH SCHOOL FUSION WORKSHOP

    Energy Technology Data Exchange (ETDEWEB)

    Punjabi, Alkesh

    2010-02-09

    Nine summer fusion science research workshops for minority and female high school students were conducted at the Hampton University Center for Fusion Research and Training from 1996 to 2005. Each workshop was of the duration of eight weeks. In all 35 high school students were mentored. The students presented 28 contributed papers at the annual meetings of the American Physical Society Division of Plasma Physics. These contributed papers were very well received by the plasma physics and fusion science research community. The students won a number of prestigious local, state, and national honors, awards, prizes, and scholarships. The notable among these are the two regional finalist positions in the 1999 Siemens-Westinghouse Science and Technology Competitions; 1st Place U.S. Army Award, 2006; 1st Place U.S. Naval Science Award, 2006; Yale Science and Engineering Association Best 11th Grade Project, 2006; Society of Physics Students Book Award, 2006; APS Corporate Minority Scholarship and others. This workshop program conducted by the HU CFRT has been an exemplary success, and served the minority and female students exceptionally fruitfully. The Summer High School Fusion Science Workshop is an immensely successful outreach activity conducted by the HU CFRT. In this workshop, we train, motivate, and provide high quality research experiences to young and talented high school scholars with emphasis on under-represented minorities and female students in fusion science and related areas. The purpose of this workshop is to expose minority and female students to the excitement of research in science at an early stage in their academic lives. It is our hope that this may lead the high school students to pursue higher education and careers in physical sciences, mathematics, and perhaps in fusion science. To our knowledge, this workshop is the first and only one to date, of fusion science for under-represented minorities and female high school students at an HBCU. The faculty

  1. Adaptive and maladaptive expression of the mRNA regulatory protein HuR

    Institute of Scientific and Technical Information of China (English)

    Suman; Govindaraju; Beth; S; Lee

    2013-01-01

    The RNA-binding proteins involved in regulation of mRNA post-transcriptional processing and translation control the fates of thousands of mRNA transcripts and basic cellular processes. The best studied of these, HuR, is well characterized as a mediator of mRNA stability and translation, and more recently, as a factor in nuclear functions such as pre-mRNA splicing. Due to HuR’s role in regulating thousands of mRNA transcripts, including those for other RNA-binding proteins, HuR can act as a master regulator of cell survival and proliferation. HuR itself is subject to multiple post-translationa modifications including regulation of its nucleocytoplasmic distribution. However, the mechanisms that govern HuR levels in the cell have only recently begun to be defined. These mechanisms are critical to cell health, as it has become clear in recent years that aberrant expression of HuR can lead alternately to decreased cell viability or to promotion of pathological proliferation and invasiveness. HuR is expressed as alternate mRNAs that vary in their untranslated regions, leading to differences in transcript stability and translatability. Multiple transcription factors and modulators of mRNA stability that regulate HuR mRNA expression have been identified. In addition, translation of HuR is regulated by numerous microRNAs, several of which have been demonstrated to have anti-tumor properties due to their suppression of HuR expression. This review summarizes the current state of knowledge of the factors that regulate HuR expression, along with the circumstances under which these factors contribute to cancer and inflammation.

  2. Fingerprint Recognition Using Local Features and Hu Moments

    Directory of Open Access Journals (Sweden)

    G. Aguilar-Torres

    2012-10-01

    Full Text Available Person identification systems based on fingerprint patterns called Automatic Fingerprint Identification Systems, AFIS,are some of the most widely used biometric methods since they provide a high degree of success. The accuracy ofAFIS is mainly due to some unique characteristics called minutiae, which are points where a curve track finishes,intersects with another curve track, or branches off. During past decades several efficient minutia-based fingerprintrecognition algorithms have been proposed which achieve false recognition rates close to 1%, however, theirrecognition rate may be still improved. To this end, this paper presents a fingerprint recognition method using acombination of the Fast Fourier Transform (FFT with Gabor filters for image enhancement. Next, fingerprintrecognition is carried out using a novel recognition stage based on Local Features and Hu invariant moments forverification.

  3. SUPPORT FOR HU CFRT SUMMER HIGH SCHOOL FUSION WORKSHOP

    Energy Technology Data Exchange (ETDEWEB)

    Punjabi, Alkesh

    2010-02-09

    Nine summer fusion science research workshops for minority and female high school students were conducted at the Hampton University Center for Fusion Research and Training from 1996 to 2005. Each workshop was of the duration of eight weeks. In all 35 high school students were mentored. The students presented 28 contributed papers at the annual meetings of the American Physical Society Division of Plasma Physics. These contributed papers were very well received by the plasma physics and fusion science research community. The students won a number of prestigious local, state, and national honors, awards, prizes, and scholarships. The notable among these are the two regional finalist positions in the 1999 Siemens-Westinghouse Science and Technology Competitions; 1st Place U.S. Army Award, 2006; 1st Place U.S. Naval Science Award, 2006; Yale Science and Engineering Association Best 11th Grade Project, 2006; Society of Physics Students Book Award, 2006; APS Corporate Minority Scholarship and others. This workshop program conducted by the HU CFRT has been an exemplary success, and served the minority and female students exceptionally fruitfully. The Summer High School Fusion Science Workshop is an immensely successful outreach activity conducted by the HU CFRT. In this workshop, we train, motivate, and provide high quality research experiences to young and talented high school scholars with emphasis on under-represented minorities and female students in fusion science and related areas. The purpose of this workshop is to expose minority and female students to the excitement of research in science at an early stage in their academic lives. It is our hope that this may lead the high school students to pursue higher education and careers in physical sciences, mathematics, and perhaps in fusion science. To our knowledge, this workshop is the first and only one to date, of fusion science for under-represented minorities and female high school students at an HBCU. The faculty

  4. “Meticulous Crafting” and “Plain Style”: Hu Shi's Prose

    Institute of Scientific and Technical Information of China (English)

    ChenPingyuan; ZhuShida; DavidKelly

    2004-01-01

    Hu Shi's Outline History of Chinese Philosophy, despite a number of shortcomings, opened a new era for an academic prose in vernacular Chinese. Hu set great store by the structural features of a """"work,"""" arguing that it was not enough to assemble quotes,

  5. A Meta Analysis and Hierarchical Classification of HU-Based Atherosclerotic Plaque Characterization Criteria

    NARCIS (Netherlands)

    Kristanto, Wisnumurti; van Ooijen, Peter M. A.; Jansen-van der Weide, Marijke C.; Vliegenthart, Rozemarijn; Oudkerk, Matthijs

    2013-01-01

    Background: Many computed tomography (CT) studies have reported that lipid-rich, presumably rupture-prone atherosclerotic plaques can be characterized according to their Hounsfield Unit (HU) value. However, the published HU-based characterization criteria vary considerably. The present study aims to

  6. HuD Regulates Coding and Noncoding RNA to Induce APP→Aβ Processing

    Directory of Open Access Journals (Sweden)

    Min-Ju Kang

    2014-06-01

    Full Text Available The primarily neuronal RNA-binding protein HuD is implicated in learning and memory. Here, we report the identification of several HuD target transcripts linked to Alzheimer’s disease (AD pathogenesis. HuD interacted with the 3′ UTRs of APP mRNA (encoding amyloid precursor protein and BACE1 mRNA (encoding β-site APP-cleaving enzyme 1 and increased the half-lives of these mRNAs. HuD also associated with and stabilized the long noncoding (lncRNA BACE1AS, which partly complements BACE1 mRNA and enhances BACE1 expression. Consistent with HuD promoting production of APP and APP-cleaving enzyme, the levels of APP, BACE1, BACE1AS, and Aβ were higher in the brain of HuD-overexpressing mice. Importantly, cortex (superior temporal gyrus from patients with AD displayed significantly higher levels of HuD and, accordingly, elevated APP, BACE1, BACE1AS, and Aβ than did cortical tissue from healthy age-matched individuals. We propose that HuD jointly promotes the production of APP and the cleavage of its amyloidogenic fragment, Aβ.

  7. Introduction to plant feng shui culture expression in landscape%植物风水文化在景观中的表达

    Institute of Scientific and Technical Information of China (English)

    黄红艳

    2015-01-01

    This article expounds the history and culture origin between the plant landscape and feng shui, and through analyze the plants feng shui feng shui culture in constructing the Chinese traditional garden and the application of modern plant landscape and expres-sion. Put forward the flexible use of the traditional Chinese fengshui theory, achieve the sustainable utilization of the natural environment, the special value of constructing modern landscape plants feng shui culture is a inevitable choice of the return of human culture.%阐述了植物景观与风水之间的历史文化渊源,并通过对植物风水的剖析阐明风水文化在构造中国传统园林和现代植物景观中的运用和表达。提出灵活运用中国传统风水学理论,实现自然环境的可持续利用,以植物风水文化的特殊价值构建现代景观是人类文化回归的必然选择。

  8. Immune response in lung cancer mouse model mimics human anti-Hu reactivity

    Science.gov (United States)

    Kazarian, Meleeneh; Calbo, Joaquim; Proost, Natalie; Carpenter, Catherine L.; Berns, Anton; Laird-Offringa, Ite A.

    2009-01-01

    Most patients with paraneoplastic encephalomyelitis/sensory neuronopathy PEM/SN have small-cell lung cancer (SCLC) and develop antibodies against neuronal-specific Hu proteins, which are abnormally expressed in the tumor. Anti-Hu reactivity is present in ~16% of SCLC patients without PEM/SN. Here we test the hypothesis that engineered SCLC-prone mice may exhibit anti-Hu reactivity. We show that tumors from SCLC-prone mice misexpress Hu proteins, and 14% of mice harbor anti-Hu antibodies. Mice appear to show reactivity prior to clinical diagnosis of SCLC. This mouse model system will be useful to study SCLC-associated autoimmunity, its diagnostic value, and the potential protective role of oncoantigen-directed autoantibodies. PMID:19765830

  9. 中国风水地理哲学基础与人地关系%Philosophical Foundation of Chinese Feng Shui Geography and Man-Land Relationship

    Institute of Scientific and Technical Information of China (English)

    孙峰华; 方创琳; 王振波; 颜廷真

    2014-01-01

    The paper firstly examines traditional Feng Shui geography from the perspective of dialectical materialism, and constructs a pattern diagram from traditional to modern Feng Shui geography based on critique of Chinese Feng Shui philosophy geography. The concept of“Tian Ren He Yi”(Man and nature in one) in“The Book of Changes”laid the philosophical foundation of traditional Feng Shui geography, owing to the functions of concept of“Tian Ren He Yi”. Modern Feng Shui geography practices have endowed the concept of“Tian Ren He Yi”with new connotation. The renewed philosophical ideology of“Tian Ren He Yi”bears important significance on Modern Feng Shui geography research, the former of the two closely related to “man-land relationship”.“Man-land relationship”is not only the research theme and core of modern human geography, but also the source and the core of the crux of modern Feng Shui geography. In this paper, the systematic rethinking of the“man-land relationship” theoretical model lays a solid theoretical foundation for the study of man-land relationship of modern Feng shui geography. And this study points out that traditional Feng Shui geography contains a large number of superstition and pseudo-scientific thoughts, and must be subjected to philosophical examination of science and innovation. Relevant modern scientific theories, methods, and techniques must be introduced, so that the study will develop from the qualitative research to empirical research, from traditional to modern Feng Shui Geography.%首先用辩证唯物主义哲学观考察了传统风水地理学,在对中国风水地理哲学批判的基础上,构建了从传统风水地理学走向现代风水地理学的模式图。《周易》中的“天人合一”观之所以奠定了传统风水地理学的哲学基础,它由“天人合一”思想的功能所决定。现代风水地理学实践赋予了“天人合一”新内涵,新“天人合一”的哲学

  10. HuR knockdown changes the oncogenic potential of oral cancer cells.

    Science.gov (United States)

    Kakuguchi, Wataru; Kitamura, Tetsuya; Kuroshima, Takeshi; Ishikawa, Makoto; Kitagawa, Yoshimasa; Totsuka, Yasunori; Shindoh, Masanobu; Higashino, Fumihiro

    2010-04-01

    HuR binds to AU-rich element-containing mRNA to protect them from rapid degradation. Here, we show that knockdown of HuR changes the oncogenic properties of oral cancer cells. Oral squamous cell carcinoma cell lines, HSC-3 and Ca9.22, which express HuR protein and cytoplasmic AU-rich element mRNA more abundantly than normal cells, were subjected to HuR knockdown. In the HuR-knockdown cancer cells, the cytoplasmic expression of c-fos, c-myc, and COX-2 mRNAs was inhibited compared with those in cells that had been transfected with a control small interfering RNA, and the half-lives of these mRNAs were shorter than those of their counterparts in the control cells. HuR-knockdown cells failed to make colonies in soft agar, suggesting that the cells had lost their ability for anchorage-independent cell growth. Additionally, the motile and invasive activities of the cells decreased remarkably by HuR knockdown. Furthermore, the expression of cell cycle-related proteins, such as cyclin A, cyclin B1, cyclin D1, and cyclin-dependent kinase 1, was reduced in HuR-knockdown cancer cells, and HuR bound to cdk1 mRNA to stabilize it. These findings suggest that HuR knockdown changes the features of oral cancer cells, at least in part, by affecting their cell cycle and shows potential as an effective therapeutic approach.

  11. HuR contributes to Hepatic Stellate Cell activation and liver fibrosis

    Science.gov (United States)

    Woodhoo, A.; Iruarrizaga-Lejarreta, M.; Beraza, N.; García-Rodríguez, J.L.; Embade, N.; Fernández-Ramos, D.; Matinez-Lopez, N.; Gutiérrez, Virginia; Arteta, B.; Caballeria, J.; Lu, S.C.; Mato, J.M.; Varela-Rey, M.; Martinez-Chantar, M.L.

    2012-01-01

    RNA-binding proteins (RBPs) play a major role in control of mRNA turnover and translation rates. We examined the role of the RBP human antigen R (HuR) during cholestatic liver injury and hepatic stellate cells (HSC) activation. HuR silencing attenuated fibrosis development in vivo after BDL, reducing liver damage, oxidative stress, inflammation, and collagen and α-SMA (α-smooth muscle actin) expression. HuR expression increased in activated HSC from BDL mice and during HSC activation in vitro, and HuR silencing markedly reduced HSC activation. HuR regulated platelet-derived growth factor (PDGF)-induced proliferation and migration, and controlled expression of several mRNAs involved in these processes (Actin, MMP9, Cyclin D1 and B1). These functions of HuR were linked to its abundance and cytoplasmic localisation, controlled by PDGF, via ERK and PI3K activation, and ERK-LKB1 activation respectively. More importantly, we identified the tumor suppressor LKB1 as a novel downstream target of PDGF-induced ERK activation in HSC. HuR also controlled transforming growth factor beta (TGF-β-induced profibrogenic actions by regulating expression of TGF-β, α-SMA, and p21. This was likely due to an increased cytoplasmic localisation of HuR, controlled by TGF-β-induced p38 MAPK activation. Finally, we found that HuR and LKB1 (Ser428) levels were highly expressed in activated HSC in human cirrhotic samples. Conclusion: Our results show that HuR is important for pathogenesis of liver fibrosis development in the cholestatic injury model, for HSC activation, and for the response of activated HSC to PDGF and TGF-β. PMID:22576182

  12. A Discussion on Images of Lyrics by Feng Yansi%冯延巳词意象浅论

    Institute of Scientific and Technical Information of China (English)

    魏玮

    2011-01-01

    冯延巳词由自然意象群和室内外建筑与装饰意象群两大意象群组成。词的意象选择与前代词相比有突出的变化。总体表现为:在一定程度上消解了以女性为中心的意象群特征,意象组合崇尚清雅,同时带有明显的萧疏、衰飒、孤寂之感。%This article researches on the images of lyrics by Feng Yansi,which composed of the natural images and images of inside outside buildings and decorations.The choice of word image had prominent changes comparing with word used in the previous dynasty.The m

  13. Regulation of the mRNA-binding protein HuR by posttranslational modification: spotlight on phosphorylation.

    Science.gov (United States)

    Eberhardt, Wolfgang; Doller, Anke; Pfeilschifter, Josef

    2012-06-01

    The ubiquitous mRNA-binding protein human antigen R (HuR) and its neuronal relatives (HuB, HuC, HuD) participate in the post-transcriptional regulation of many AU-rich element-bearing mRNAs. In addition to its originally described role in controlling mRNA decay, the binding of HuR to target mRNAs can affect many aspects of mRNA processing including splicing, polyadenylation, intracellular trafficking, translation and modulation of mRNA repression by miRNAs. In accordance to the growing list of signalling events which are involved in regulating these different HuR functions, recent data implicate that posttranslational modification, namely protein kinase-triggered phosphorylation of HuR plays a crucial role in connecting extracellular signal inputs to a specific post-transcriptional program by HuR. Notably, in addition to directly targeting HuR functions, posttranslational modifications of HuR have a major impact on the sequestration and binding to various HuR ligand proteins as has been demonstrated e.g. for the 14-3-3 chaperones. However, the detailed mechanisms of how a specific modification of HuR coordinates different aspects in HuR regulation are currently poorly understood. Due to the fact that most of the described HuR activities are closely related to its subcellular localization and the binding to cargo mRNA, this review will focus on these aspects of HuR functions and their control by posttranslational modification, particularly by HuR phosphorylations by different protein kinases.

  14. Landscape in the Soul-On the Prose Collection Landscape of Feng Zhi%灵魂里的山水--论冯至散文集《山水》

    Institute of Scientific and Technical Information of China (English)

    陈邑华

    2015-01-01

    Feng Zhi has been hailed by Lu Xun as'the most distinguished lyric poet of China'.His prose collection Landscape is a unique existence in Chinese modern prose.During the War of Resist-ance Against Japan,the landscape in a plain and quiet corner in Kunming became the spiritual home of Feng Zhi.He absorbed the ideas in Chinese Zen and accretion of'the world and me,and the union of all and me'in Taoism,blended with the landscape concept from Rilke and the Metamorphosis Theory of Goethe,to form his original view of nature which is rich in'universe spirit',and that is all are in u-nion,from the same root,and moving upwards and forward in integration.This view has a strong hu-manistic color.In Landscape ,it focuses on'landscape in the soul',with the flowing feeling of benign and open-hearted,and the theme of'Right of Life and Death'running through.This wise and mag-nanimous view of life and death is full of personality strength of responsibility,perseverance and posi-tivity.It has a clear and deeply graceful writing style,with the fusion of poetics and philosophy,as well as the lingering charm,like a meditative poetry.%冯至被鲁迅誉为“中国最为杰出的抒情诗人”,其散文集《山水》在中国现代散文中可谓是一个独特的存在。抗战时期,昆明一隅素朴、安静的自然山水成为冯至的精神寓所,冯至汲取中国禅宗、道家“天地与我并生,万物与我一体”的思想,融合里尔克的山水理念以及歌德的蜕变论,形成自己独到的富于“宇宙精神”的自然观,即众生一体、万有同源,一切均在关联变化中向前向上的自然观。这一自然观具有强烈的人本主义色彩。《山水》关注“灵魂里的山川”,流淌着光风霁月的情怀,贯穿着“正当的死生”的主旋律。这一睿智坦荡的生死观,充溢着担当、坚忍、积极向上的人格力量。《山水》行文清朗深婉,诗情与哲思水乳交融,韵味悠长,宛如一首沉思的诗。

  15. Structural characterization of the maytansinoid–monoclonal antibody immunoconjugate, huN901–DM1, by mass spectrometry

    National Research Council Canada - National Science Library

    Wang, Lintao; Amphlett, Godfrey; Blättler, Walter A; Lambert, John M; Zhang, Wei

    2005-01-01

    ...‐specific drug delivery. The immunoconjugate, huN901–DM1, composed of the humanized monoclonal IgG 1 antibody, huN901, and the maytansinoid drug, DM1, is being tested in clinical trials to treat small cell lung carcinoma (SCLC). huN901...

  16. Measurement of hard tissue density of head phantom based on the HU by using CBCT

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Moon Sun; Kang, Dong Wan; Kim, Jae Duk [School of Dentistry, Chosun University, Gwangju (Korea, Republic of)

    2009-09-15

    The purpose of this study was to determine a conversion coefficient for Hounsfield Units(HU) to material density (g cm{sup -3}) obtained from cone-beam computed tomography (CBMercuRay{sup TM}) data and to measure the hard tissue density based on the Hounsfield scale on dental head phantom. CT Scanner Phantom (AAPM) equipped with CT Number Insert consists of five cylindrical pins of materials with different densities and teflon ring was scanned by using the CBMercuRay{sup TM} (Hitachi, Tokyo, Japan) volume scanner. The raw data were converted into DICOM format and the HU of different areas of CT number insert measured by using CBWorks{sup TM}. Linear regression analysis and Student t-test were performed statistically. There was no significant difference (P>0.54) between real densities and measured densities. A linear regression was performed using the density, {rho} (g cm{sup -3}), as the dependent variable in terms of the HU (H). The regression equation obtained was {rho}=0.00072 H-0.01588 with an R2 value of 0.9968. Density values based on the Hounsfield scale was 1697.1 {+-} 24.9 HU in cortical bone, 526.5 {+-} 44.4 HU in trabecular bone, 2639.1 {+-} 48.7 HU in enamel, 1246.1 {+-} 39.4 HU in dentin of dental head phantom. CBCT provides an effective option for determination of material density expressed as Hounsfield Units.

  17. On the ephemeris of the eclipsing polar HU Aquarii

    CERN Document Server

    Schwope, A D

    2014-01-01

    The magnetic cataclysmic variable HU Aquarii displayed pronounced quasi-periodic modulations of its eclipse timing. These were interpreted in terms of the light-travel time (LTT) effect caused by a circumbinary planet or planetary system. We report new photometric observations that revealed another precise eclipse timing for the October 2013 epoch, the first obtained in a high accretion state after many years in low or intermediate states. The eclipse was observed to occur earlier by 95.3 +- 2.0 s or 62.8 +- 2.0 s than expected for an assumed linear or quadratic ephemeris, respectively. The implied apparent strong evolution of the orbital period calls for a revision of the current planetary model or the planetary parameters. The object deserves further monitoring to uncover the true nature of the observed variability and to constrain the properties of the proposed planet or planetary system. The new observations prove that advanced amateur equipment can successfully be used in the growing field of planet sear...

  18. HuR contributes to Hepatic Stellate Cell activation and liver fibrosis

    OpenAIRE

    Woodhoo, A.; Iruarrizaga-Lejarreta, M.; Beraza, N.; García-Rodríguez, J.L.; Embade, N.; Fernández-Ramos, D.; Matinez-Lopez, N.; Gutiérrez, Virginia; Arteta, B; Caballeria, J.; Lu, S.C. (Shelly C.); Mato, J.M. (José María); Varela-Rey, M.; Martinez-Chantar, M.L.

    2012-01-01

    RNA-binding proteins (RBPs) play a major role in control of mRNA turnover and translation rates. We examined the role of the RBP human antigen R (HuR) during cholestatic liver injury and hepatic stellate cells (HSC) activation. HuR silencing attenuated fibrosis development in vivo after BDL, reducing liver damage, oxidative stress, inflammation, and collagen and α-SMA (α-smooth muscle actin) expression. HuR expression increased in activated HSC from BDL mice and during HSC activation in vitro...

  19. Ms HU Ling-xiang's Experience in Treating Blood Stasis by Acupuncture Balancing Method

    Institute of Scientific and Technical Information of China (English)

    FU Jia; LI Ji; ZHANG Cai-rong

    2005-01-01

    @@ HU Ling-xiang, 60, is a chief doctor and supervisor of postgraduates of master degree. She specializes in the syndrome differentiation and treatment of different kinds of chronic and difficult disorders from the perspective of blood stasis theory.

  20. Zebrafish transgenic line huORFZ is an effective living bioindicator for detecting environmental toxicants.

    Directory of Open Access Journals (Sweden)

    Hung-Chieh Lee

    Full Text Available Reliable animal models are invaluable for monitoring the extent of pollution in the aquatic environment. In this study, we demonstrated the potential of huORFZ, a novel transgenic zebrafish line that harbors a human upstream open reading frame of the chop gene fused with GFP reporter, as an animal model for monitoring environmental pollutants and stress-related cellular processes. When huORFZ embryos were kept under normal condition, no leaked GFP signal could be detected. When treated with hazardous chemicals, including heavy metals and endocrine-disrupting chemicals near their sublethal concentrations (LC50, huORFZ embryos exhibited different tissue-specific GFP expression patterns. For further analysis, copper (Cu2+, cadmium (Cd2+ and Chlorpyrifos were applied. Cu2+ triggered GFP responses in skin and muscle, whereas Cd2+ treatment triggered GFP responses in skin, olfactory epithelium and pronephric ducts. Moreover, fluorescence intensity, as exhibited by huORFZ embryos, was dose-dependent. After surviving treated embryos were returned to normal condition, survival rates, as well as TUNEL signals, returned to pretreatment levels with no significant morphological defects observed. Such results indicated the reversibility of treatment conditions used in this study, as long as embryos survived such conditions. Notably, GFP signals decreased along with recovery, suggesting that GFP signaling of huORFZ embryos likely reflected the overall physiological condition of the individual. To examine the performance of the huORFZ line under real-world conditions, we placed huORFZ embryos in different river water samples. We found that the huORFZ embryos correctly detected the presence of various kinds of pollutants. Based on these findings, we concluded that such uORFchop-based system can be integrated into a first-line water alarm system monitoring the discharge of hazardous pollutants.

  1. Hu'rthle Cell Thyroid Adenoma with an Eggshell Calcification: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dong Gil; Lee, Sang Kwon; Choe, Mi Sun; Chang, Hyuk Won; Rho, Byung Hak [Dongsan Medical Center, Keimyung University School of Medicine, Daegu (Korea, Republic of)

    2009-09-15

    We report here a case of Hu'rthle cell adenoma with eggshell calcification that presented as a thyroid incidentaloma on ultrasonography (US) in a 58-year-old woman. The mass was hypoechoic with continuous eggshell calcification and intranodular vascularity as seen on gray-scale and power Doppler (PD) US. Hu 'rthle cell adenoma should be considered in the differential diagnosis of a thyroid nodule with eggshell calcification

  2. 绍兴鲁迅故居风水浅析%Analysis Of Feng-Shui Of Shaoxing Lu Xun's Former Residence

    Institute of Scientific and Technical Information of China (English)

    张耀升; 罗一萍

    2016-01-01

    中国传统民居营建过程中包含着风水的思想,本文以绍兴周家新台门出卖给东邻朱家前绘制的平面图为研究对象,从周边环境、宅形、营建理念等对绍兴鲁迅故居进行风水浅析。%The construction of Chinese traditional houses include the idea of Feng-shui.The Building plan before the sale of Shaoxing Lu Xun's former residence. Based on this, the paper analysis of Feng-shui of Shaoxing Lu Xun's former residence from the surounding environment, the house shape, the theory of construction.

  3. 论风景园林设计中的风水观%The Outlook of Feng-shui in Landscape Architecture Design

    Institute of Scientific and Technical Information of China (English)

    王一飞; 马英豪; 叶文学; 郝应林

    2013-01-01

      在分析了风水学的科学性和民俗文化性的基础上,提出了树立和弘扬全新的风水观才能使风水学在现代中国民族风景园林设计中发挥其应有的作用。%  On the analysis of the geomantic learn is scientific and the folk custom culture, on the basis of the proposed set up and carry forward the new concept of Feng-shui to Feng-shui in the landscape design of mod⁃ern Chinese people play its proper role.

  4. 傅友丰教授治疗闭经经验%Experience of FU You-feng Professor Treatment Amenorrhea

    Institute of Scientific and Technical Information of China (English)

    周红光

    2012-01-01

    对傅友丰教授治疗闭经临床经验进行总结,介绍其治病特色.傅友丰教授治疗闭经强调辨证分虚实;补肾调周,采用中药建立人工周期;补肾填精,健脾和中贯穿始终等方法,取得很好的临床效果,值得临床推广应用.%This paper summarized FU You-feng professor of clinical experience in the treatment of amenorrhea, introduced her Cure characteristics. FUYou-feng professor emphasize that syndrome differentiation distinguish deficiency and exces; nourish kidney use of Chinese medicine build artificial cycle and nourish kidney replenishing essence. Acquired very good clinical effect and deserved clinical application.

  5. Neuroprotective effects of the cannabinoid agonist HU210 on retinal degeneration.

    Science.gov (United States)

    Lax, Pedro; Esquiva, Gema; Altavilla, Cesare; Cuenca, Nicolás

    2014-03-01

    Cannabinoids have been demonstrated to exert neuroprotective effects on different types of neuronal insults. Here we have addressed the therapeutic potential of the synthetic cannabinoid HU210 on photoreceptor degeneration, synaptic connectivity and functional activity of the retina in the transgenic P23H rat, an animal model for autosomal dominant retinitis pigmentosa (RP). In P23H rats administered with HU210 (100 μg/kg, i.p.) from P24 to P90, ERG recordings showed an amelioration of vision loss, as compared to vehicle-administered animals. Under scotopic conditions, the maximum a-wave amplitudes recorded at P60 and P90 were higher in HU210-treated animals, as compared to the values obtained in untreated animals. The scotopic b-waves were significantly higher in treated animals than in untreated rats at P30, P60 and P90. This attenuation of visual deterioration correlated with a delay in photoreceptor degeneration and the preservation of retinal cytoarchitecture. HU210-treated animals had 40% more photoreceptors than untreated animals. Presynaptic and postsynaptic elements, as well as the synaptic contacts between photoreceptors and bipolar or horizontal cells, were also preserved in HU210-treated P23H rats. These results indicate that HU210 preserves cone and rod structure and function, together with their contacts with postsynaptic neurons, in P23H rats. These data suggest that cannabinoids are potentially useful to delay retinal degeneration in RP patients.

  6. Pertussis toxin B-oligomer inhibits HIV infection and replication in hu-PBL-SCID mice.

    Science.gov (United States)

    Lapenta, Caterina; Spada, Massimo; Santini, Stefano M; Racca, Sara; Dorigatti, Fernanda; Poli, Guido; Belardelli, Filippo; Alfano, Massimo

    2005-04-01

    Bordetella pertussis toxin B-oligomer (PTX-B) has been shown to inhibit HIV infection and replication in vitro. The potential anti-viral effect of PTX-B was tested here in an in vivo surrogate model of HIV infection, i.e. SCID mice reconstituted with human peripheral blood leukocytes (PBL) (hu-PBL-SCID) and infected with a CCR5-dependent (R5) HIV-1 strain. SCID mice inoculated intra-peritoneal (i.p.) with PTX-B and then infected with the R5 strain SF-162 were sacrificed 7 days later and analyzed for human PBL (hu-PBL) lymphoid tissue reconstitution, infection of hu-PBL, plasma viremia and viral rescue from ex vivo-cultivated i.p. hu-PBL. Unlike mice treated with 500 ng per animal of PTX-B showing no evidence of viral inhibition, daily administration of PTX-B (50 ng per mouse) strongly inhibited virus infection and replication, as determined by undetectable viremia, absence of infected hu-PBL and lack of rescue of infectious HIV in most animals. Furthermore, PTX-B injection 2 h before and twice after infection prevented HIV-1 infection and replication in all (10/10) tested animals. Thus, PTX-B potently inhibited virus infection and replication in hu-PBL-SCID mice, supporting the hypothesis that it may represent a new pharmacological agent against HIV-1 infection.

  7. Different modes of interaction by TIAR and HuR with target RNA and DNA.

    Science.gov (United States)

    Kim, Henry S; Wilce, Matthew C J; Yoga, Yano M K; Pendini, Nicole R; Gunzburg, Menachem J; Cowieson, Nathan P; Wilson, Gerald M; Williams, Bryan R G; Gorospe, Myriam; Wilce, Jacqueline A

    2011-02-01

    TIAR and HuR are mRNA-binding proteins that play important roles in the regulation of translation. They both possess three RNA recognition motifs (RRMs) and bind to AU-rich elements (AREs), with seemingly overlapping specificity. Here we show using SPR that TIAR and HuR bind to both U-rich and AU-rich RNA in the nanomolar range, with higher overall affinity for U-rich RNA. However, the higher affinity for U-rich sequences is mainly due to faster association with U-rich RNA, which we propose is a reflection of the higher probability of association. Differences between TIAR and HuR are observed in their modes of binding to RNA. TIAR is able to bind deoxy-oligonucleotides with nanomolar affinity, whereas HuR affinity is reduced to a micromolar level. Studies with U-rich DNA reveal that TIAR binding depends less on the 2'-hydroxyl group of RNA than HuR binding. Finally we show that SAXS data, recorded for the first two domains of TIAR in complex with RNA, are more consistent with a flexible, elongated shape and not the compact shape that the first two domains of Hu proteins adopt upon binding to RNA. We thus propose that these triple-RRM proteins, which compete for the same binding sites in cells, interact with their targets in fundamentally different ways.

  8. [Mining analysis and experience summary for chronic atrophic gastritis cases treated by Professor LIU Feng-bin].

    Science.gov (United States)

    Hou, Zheng-kun; Liu, Feng-bin; Li, Pei-wu; Zhuang, Kun-hai

    2015-06-01

    To summarize Professor LIU Feng-bin's clinical experience and theoretical thoughts on chronic atrophic gastritis (CAG), the study group designed a retrospective study on his case series and expert interview. First of all, the data of CAG patients treated in the First Affiliated Hospital of Guangzhou University of Chinese Medicine between 2009 and 2013, e. g. herbs, diseases, syndrome type, prescription amount and number of herbs, was collected and processed. The statistical description and binary logistic regression were used to determined the syndrome type, initial basic remedy and modification. During the statistics, a complete and sub-group analysis was performed simultaneously. After the expert interview, the syndrome type and medication were finalized. As a result, a total of 228 CAG patients aged at (50.30 ± 10.18) were collected, including 151 males (66.23%). Of them, the TCM diagnosis and syndrome type were extracted from the information of 157 patients, including 115 cases with gastric stuffiness, 23 cases with gastric pain, 19 missing cases, 2 cases with spleen-stomach weakness syndrome, 57 cases with spleen deficiency and dampness-heat syndrome, 18 cases with spleen-stomach disharmony syndrome, 23 cases with syndrome of liver depression syndrome, 21 cases with liver qi invading stomach syndrome and 26 qi and yin deficiency syndrome, respectively. All of the 228 patients used totally 104 herbs, while the subgroups with 157 patients used 94 herbs. The most frequently used 15 herbs used in each groups were analyzed to determine the initial basic remedy and modification. Subsequently, based on the information of the sub-groups with 157 patients, with the syndrome type as the dependent variable, the logistic regression analysis was made on the most frequently used 32 herbs, in order to determined the modification in herbs for different syndrome types. After experts reviewed and modified, they believed the main causes of CAG were dietary irregularities

  9. 论我国古代城市规划中的风水观%The Feng-shui Theory in the Urban Planning of Ancient China

    Institute of Scientific and Technical Information of China (English)

    赵景伟

    2011-01-01

    风水在我国城市选址、城市规划、建筑设计、园林设计和营造中都有所体现,中国古都风貌的精髓就是在古代城市规划布局中所体现出来的风水观念。《周易》、《管子》以及道家思想,是中国古代风水理论产生的思想背景,其阴阳哲学思想在古代城市规划中具有非常显著的运用,风水理论所体现的生态观念仍然是现代城市规划的目标之一。正确运用风水理论中科学的观念去正确处理城市规划和环境问题,是当前国内外规划和设计人员所面临的任务。%The theory of Feng-shui, the essence of Chinese ancient city landscape, was adequately reflected in the urban location, the urban planning, architectural design, gardening design and building in ancient China. The Yin-Yang philosophy in Feng-shui theory, which is derived from the thoughts of Zhouyi, Guanzi and Taoism, was widely applied in ancient urban planning, and the ecological idea in Feng-shui is applicable even in today's urban planning. It is believed that ideas in Feng-shui theory should be used scientifically to solve the environment problem coming of urban planning and desig ning, with which designers and planners home and abroad are confronted.

  10. 雷锋精神与竞技体育道德重塑%Spirit of Lei Feng and the Competitive Sports Moral Reconstruction

    Institute of Scientific and Technical Information of China (English)

    宋惠娟

    2012-01-01

    运用文献资料法和逻辑分析法,对雷锋精神视角下的我国竞技体育道德重塑进行深入研究。首先简析了雷锋精神的时代内涵。其次对雷锋精神视角下的我国竞技体育理论道德缺失特征进行了全面分析,其中主要包括:不敬业而敬"金",诚信和公平竞赛精神的缺失;崇尚形象,抛弃节俭,甚至不计成本;个人与集体矛盾重重三大方面。最后,指出了以雷锋精神重塑我国竞技体育道德的相关对策与建议。%With the research methods of literature study and logical analysis,the paper has an in-depth study on the competitive sports moral reconstruction of China from the perspective of Lei Feng spirit.The paper analyses the time connotation of LEI Feng Spirit and has a comprehensive analysis of moral deficiency of competitive sport theory in China from the perspective of LEI Feng Spirit.The paper proposes the reasons of the deficiency,such as more thinking of money than work,lack of the spirit of faith and fair competition;image advocating,thrift abandoning and the conflicts between the individual and the group.The paper,points out the countermeasures and suggestions about the reconstruction of China's competitive sports morality through LEI Feng Spirit.

  11. Concept Of Feng Shui In The Application Of Landscape Design%风水理念在园林设计中的应用

    Institute of Scientific and Technical Information of China (English)

    闫俊文; 刘庭风

    2016-01-01

    today, the landscape design should consider many aspects, feng shui is one of the important factors about the rationality of the landscape planning, even wil involve the problem of good or il luck, should be taken seriously, and strengthen the relevant theoretical research. This article first to feng shui theory and its basic principle to do a simple introduction, and from the site selection, landscape design, plant configure three aspects analyzes the practical application of the concept of feng shui.%如今园林设计要综合考虑很多方面,风水是其中一个重要因素,关乎园林规划的合理性,甚至会涉及到吉凶问题,应当引起重视,并加强相关理论研究。本文先对风水理论及其基本原则做了简单介绍,然后从选址立基、景观设计、植物配置三方面分析了风水理念的实际应用。

  12. 风水理论中的美学原则及审美取向%The Aesthetic Principles and Standards of Feng Shui Theory

    Institute of Scientific and Technical Information of China (English)

    肖冠兰

    2012-01-01

    Feng Shui is the most important theory system of Chinese traditional culture to estimate the natural environment for site selection. The main aesthetic principles and standards of Feng Shui such as clear priorities, balance and stability, etc. will be analyzed in this paper. And it will also propose that the aesthetic principles and standards of Feng Shui theory play a guiding role in the organization and construction of the classical architectures.%本文通过分析风水理论中对自然环境的评价原则,指出其中所包含的“主次分明”、“均衡稳定”、“宽阔大气”、“屈曲灵动”等美学思想和审美取向及其对中国古典建筑的环境景观、空间构成、建筑群体组合等的指导作用。

  13. Study on the art of language of Feng Gong's sketch%冯巩小品语言艺术的研究

    Institute of Scientific and Technical Information of China (English)

    李馥君

    2013-01-01

    Feng Gong's sketch was a public concern, the main reason were the characteristics of the language approach, humorous joke, vivid interpretation. So his sketch loved by all of us. In this article, the language of Feng Gong's sketch in recent years are analyzed and explored by the voice pronunciation, vocabulary and sentence structure .And summarizes the expression features of the characteristics of the language of the Feng Gong's sketch.%冯巩的小品被大众所关注,主要是因为特色化的语言方式,诙谐幽默的笑料,生动形象的演绎,使得他的小品深受大家的喜爱。本文主要通过语音、词汇、句式方面将冯巩近几年小品的语言进行分析和探究,概括出冯巩小品特色化语言的表达特点。

  14. On the Style Transformation in Feng Chih's Poems before 1949%论冯至1949年前诗歌风格的转变

    Institute of Scientific and Technical Information of China (English)

    杨洋

    2013-01-01

    Before the year of 1949, Feng Chih's poetry writing went through a great transformation from "Romanticism Lyrics"to"Modernism Lyrics".The poetries written between 1920s and 1930s were different from those in 1940s in the intensity of the subject spirit's interposing , the description objects , and the implications .When Feng Chih was in Germany , he had been deeply influenced by Rilke, a German poet, and the philosophy of "existentialism"prevailing at that time in Germany , both of which had also played an innegligible part in the transformation of Feng Chih's poetic style .%冯至的诗歌创作在1949年前经历了一次由“浪漫主义抒情诗”向“现代主义抒情诗”的巨大转变,二十至三十年代的诗歌与四十年代的诗歌在主体精神的介入程度、描写对象和思想意蕴等方面发生了变化。在冯至留德期间,德语诗人里尔克以及盛行于当时德国的存在主义哲学对冯至的创作产生了巨大影响,并且在冯至诗风转变中起到不可忽视的作用。

  15. A Module Analysis Approach to Investigate Molecular Mechanism of TCM Formula: A Trial on Shu-feng-jie-du Formula

    Directory of Open Access Journals (Sweden)

    Jianglong Song

    2013-01-01

    Full Text Available At the molecular level, it is acknowledged that a TCM formula is often a complex system, which challenges researchers to fully understand its underlying pharmacological action. However, module detection technique developed from complex network provides new insight into systematic investigation of the mode of action of a TCM formula from the molecule perspective. We here proposed a computational approach integrating the module detection technique into a 2-class heterogeneous network (2-HN which models the complex pharmacological system of a TCM formula. This approach takes three steps: construction of a 2-HN, identification of primary pharmacological units, and pathway analysis. We employed this approach to study Shu-feng-jie-du (SHU formula, which aimed at discovering its molecular mechanism in defending against influenza infection. Actually, four primary pharmacological units were identified from the 2-HN for SHU formula and further analysis revealed numbers of biological pathways modulated by the four pharmacological units. 24 out of 40 enriched pathways that were ranked in top 10 corresponding to each of the four pharmacological units were found to be involved in the process of influenza infection. Therefore, this approach is capable of uncovering the mode of action underlying a TCM formula via module analysis.

  16. Streptomyces pactum assisted phytoremediation in Zn/Pb smelter contaminated soil of Feng County and its impact on enzymatic activities

    Science.gov (United States)

    Ali, Amjad; Guo, Di; Mahar, Amanullah; Ma, Fang; Li, Ronghua; Shen, Feng; Wang, Ping; Zhang, Zengqiang

    2017-04-01

    Anthropogenic activities, such as industrial expansion, smelting, mining and agricultural practices, have intensified the discharge of potentially toxic trace elements (PTEs) into the environment, threatening human health and other organisms. To assist phytoremediation by sorghum in soil contaminated by smelters/mines in Feng County (FC), a pot experiment was performed to examine the phytoremediation potential of Streptomyces pactum (Act12) + biochar. The results showed that root uptake of Zn and Cd was reduced by 45 and 22%, respectively, while the uptake of Pb and Cu increased by 17 and 47%, respectively. The shoot and root dry weight and chlorophyll content improved after Act12 inoculation. β-glucosidase, alkaline phosphatase and urease activities in soil improved and antioxidant activities (POD, PAL, PPO) decreased after application of Act12 + biochar due to a reduction in stress from PTEs. BCF, TF and MEA confirmed the role of Act12 in the amelioration and translocation of PTEs. PCA analysis showed a correlation between different factors that affect the translocation of PTEs. Overall, Act12 promoted the phytoremediation of PTEs. Field experiments on Act12 + biochar may provide new insights into the rehabilitation and restoration of soils contaminated by mines.

  17. Research Review on Pu Feng and the Others%蒲风研究综述及其他

    Institute of Scientific and Technical Information of China (English)

    杨俏凡

    2012-01-01

    蒲风是20世纪30年代重要的左翼诗人,是中国诗歌会的骨干人物,但当下的学术界一直缺少对他的关注。通过对蒲风的创作情况、蒲风的研究现状以及蒲风研究的意义三个方面进行介绍分析,以方便更多的研究者去探究蒲风的艺术世界。%Pu Feng was an important leftwing poet in 1930s. He was regarded as the backbone in China Poetry Association. But the current academic circle had not paid much attention to him and his works for a long time. This article makes an introductory analysis its significance in order to help more on the base of the poet himself, his writing career, current study on him and researchers enter his literature world.

  18. Streptomyces pactum assisted phytoremediation in Zn/Pb smelter contaminated soil of Feng County and its impact on enzymatic activities

    Science.gov (United States)

    Ali, Amjad; Guo, Di; Mahar, Amanullah; Ma, Fang; Li, Ronghua; Shen, Feng; Wang, Ping; Zhang, Zengqiang

    2017-01-01

    Anthropogenic activities, such as industrial expansion, smelting, mining and agricultural practices, have intensified the discharge of potentially toxic trace elements (PTEs) into the environment, threatening human health and other organisms. To assist phytoremediation by sorghum in soil contaminated by smelters/mines in Feng County (FC), a pot experiment was performed to examine the phytoremediation potential of Streptomyces pactum (Act12) + biochar. The results showed that root uptake of Zn and Cd was reduced by 45 and 22%, respectively, while the uptake of Pb and Cu increased by 17 and 47%, respectively. The shoot and root dry weight and chlorophyll content improved after Act12 inoculation. β-glucosidase, alkaline phosphatase and urease activities in soil improved and antioxidant activities (POD, PAL, PPO) decreased after application of Act12 + biochar due to a reduction in stress from PTEs. BCF, TF and MEA confirmed the role of Act12 in the amelioration and translocation of PTEs. PCA analysis showed a correlation between different factors that affect the translocation of PTEs. Overall, Act12 promoted the phytoremediation of PTEs. Field experiments on Act12 + biochar may provide new insights into the rehabilitation and restoration of soils contaminated by mines. PMID:28387235

  19. Functional characterization of the HuR:CD83 mRNA interaction.

    Directory of Open Access Journals (Sweden)

    Dorothea Pieper

    Full Text Available Maturation of dendritic cells (DC is characterized by expression of CD83, a surface protein that appears to be necessary for the effective activation of naïve T-cells and T-helper cells by DC. Lately it was shown that CD83 expression is regulated on the posttranscriptional level by interaction of the shuttle protein HuR with a novel posttranscriptional regulatory RNA element (PRE, which is located in the coding region of the CD83 transcript. Interestingly, this interaction commits the CD83 mRNA to efficient nuclear export via the CRM1 pathway. To date, however, the structural basis of this interaction, which potentially involves three distinct RNA recognition motifs (RRM1-3 in HuR and a complex three-pronged RNA stem-loop element in CD83 mRNA, has not been investigated in detail. In the present work we analyzed this interaction in vitro and in vivo using various HuR- and CD83 mRNA mutants. We are able to demonstrate that both, RRM1 and RRM2 are crucial for binding, whereas RRM3 as well as the HuR hinge region contributed only marginally to this protein:RNA interaction. Furthermore, mutation of uridine rich patches within the PRE did not disturb HuR:CD83 mRNA complex formation while, in contrast, the deletion of specific PRE subfragments from the CD83 mRNA prevented HuR binding in vitro and in vivo. Interestingly, the observed inhibition of HuR binding to CD83 mRNA does not lead to a nuclear trapping of the transcript but rather redirected this transcript from the CRM1- towards the NXF1/TAP-specific nuclear export pathway. Thus, the presence of a functional PRE permits nucleocytoplasmic trafficking of the CD83 transcript via the CRM1 pathway.

  20. Hydroxyurea (HU) for prevention of recurrent stroke in sickle cell anemia (SCA).

    Science.gov (United States)

    Sumoza, Abraham; de Bisotti, Renate; Sumoza, David; Fairbanks, Virgil

    2002-11-01

    Cerebrovascular accident (CVA) is a major cause of morbidity and death in sickle cell anemia (SCA). Transfusion of packed erythrocytes is widely used to prevent this complication. However, chronic transfusion may lead to iron overload, alloimmunization, or infections. Cost and compliance may compromise transfusion therapy. A possible alternative, the prophylactic use of hydroxyurea (HU), has not been tried to determine whether it may prevent recurrent stroke. We used HU in five children with SCA who had suffered stroke, in three of them after a first episode and in the other two after a second CVA. Four had infarctive stroke and one a transient ischemic attack (TIA). Four patients took HU at a dose of 40 mg/kg/d, one patient at 30 mg/kg/d. None of the patients had recurrent stroke during 42-112 months of observation. None experienced pain crises. In all, HbF increased significantly and was maintained above 14.7% during treatment. The total Hb concentration increased 19.5 g/L (median) above the value before treatment. HU was well tolerated. None of the five children had leukopenia or thrombocytopenia during therapy. HU appears to prevent recurrence of stroke in SCA without risk of major toxicity.

  1. dNTP deficiency induced by HU via inhibiting ribonucleotide reductase affects neural tube development.

    Science.gov (United States)

    Guan, Zhen; Wang, Xiuwei; Dong, Yanting; Xu, Lin; Zhu, Zhiqiang; Wang, Jianhua; Zhang, Ting; Niu, Bo

    2015-02-03

    Exposure to environmental toxic chemicals in utero during the neural tube development period can cause developmental disorders. To evaluate the disruption of neural tube development programming, the murine neural tube defects (NTDs) model was induced by interrupting folate metabolism using methotrexate in our previous study. The present study aimed to examine the effects of dNTP deficiency induced by hydroxyurea (HU), a specific ribonucleotide reductase (RNR) inhibitor, during murine neural tube development. Pregnant C57BL/6J mice were intraperitoneally injected with various doses of HU on gestation day (GD) 7.5, and the embryos were checked on GD 11.5. RNR activity and deoxynucleoside triphosphate (dNTP) levels were measured in the optimal dose. Additionally, DNA damage was examined by comet analysis and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. Cellular behaviors in NTDs embryos were evaluated with phosphorylation of histone H3 (PH-3) and caspase-3 using immunohistochemistry and western blot analysis. The results showed that NTDs were observed mostly with HU treatment at an optimal dose of 225 mg/kg b/w. RNR activity was inhibited and dNTP levels were decreased in HU-treated embryos with NTDs. Additionally, increased DNA damage, decreased proliferation, and increased caspase-3 were significant in NTDs embryos compared to the controls. Results indicated that HU induced murine NTDs model by disturbing dNTP metabolism and further led to the abnormal cell balance between proliferation and apoptosis.

  2. Multiple Functions of the RNA-Binding Protein HuR in Cancer Progression, Treatment Responses and Prognosis

    Directory of Open Access Journals (Sweden)

    Baocheng Wang

    2013-05-01

    Full Text Available The human embryonic lethal abnormal vision-like protein, HuR, is a member of the Hu family of RNA-binding proteins. Over the past decade, this ubiquitously expressed protein has been extensively investigated in cancer research because it is involved in the regulation of mRNA stability and translation in many cell types. HuR activity and function is associated with its subcellular distribution, transcriptional regulation, translational and post-translational modifications. HuR regulation of target mRNAs is based on the interaction between the three specific domains of HuR protein and one or several U- or AU-rich elements (AREs in the untranslated region of target mRNAs. A number of cancer-related transcripts containing AREs, including mRNAs for proto-oncogenes, cytokines, growth factors, and invasion factors, have been characterized as HuR targets. It has been proposed that HuR has a central tumorigenic activity by enabling multiple cancer phenotypes. In this review, we comprehensively survey the existing evidence with regard to the diverse functions of HuR in caner development and progression. The current data also suggest that HuR might be a novel and promising therapeutic target and a marker for treatment response and prognostic evaluation.

  3. Temporally defined neocortical translation and polysome assembly are determined by the RNA-binding protein Hu antigen R.

    Science.gov (United States)

    Kraushar, Matthew L; Thompson, Kevin; Wijeratne, H R Sagara; Viljetic, Barbara; Sakers, Kristina; Marson, Justin W; Kontoyiannis, Dimitris L; Buyske, Steven; Hart, Ronald P; Rasin, Mladen-Roko

    2014-09-09

    Precise spatiotemporal control of mRNA translation machinery is essential to the development of highly complex systems like the neocortex. However, spatiotemporal regulation of translation machinery in the developing neocortex remains poorly understood. Here, we show that an RNA-binding protein, Hu antigen R (HuR), regulates both neocorticogenesis and specificity of neocortical translation machinery in a developmental stage-dependent manner in mice. Neocortical absence of HuR alters the phosphorylation states of initiation and elongation factors in the core translation machinery. In addition, HuR regulates the temporally specific positioning of functionally related mRNAs into the active translation sites, the polysomes. HuR also determines the specificity of neocortical polysomes by defining their combinatorial composition of ribosomal proteins and initiation and elongation factors. For some HuR-dependent proteins, the association with polysomes likewise depends on the eukaryotic initiation factor 2 alpha kinase 4, which associates with HuR in prenatal developing neocortices. Finally, we found that deletion of HuR before embryonic day 10 disrupts both neocortical lamination and formation of the main neocortical commissure, the corpus callosum. Our study identifies a crucial role for HuR in neocortical development as a translational gatekeeper for functionally related mRNA subgroups and polysomal protein specificity.

  4. Dormancy Properties of Paeonia ostii ‘Feng Dan’%凤丹种子休眠特性研究

    Institute of Scientific and Technical Information of China (English)

    郭丽萍; 张延龙; 牛立新; 罗建让

    2016-01-01

    This research focused on the dormancy traits of the seeds of a tree peony cultivar,Paeonia ostii ‘Feng Dan’,by studying the water permeability of the seed coat,form and vitality of the seed embryo as well as activity of the endogenous inhibitors.The results indicated that the seed coat hindered the speed of water absorption,but it did not affect the final water absorbance ratio of the seeds.The seeds were saturated after they were immersed in water for 48 hours,from which the germination rate would not be enhanced by de-coating or acid degradation treatments.The vitality of the mature seeds could reach to as high as 95.6%.However,the embryos were not fully developed,stratification treatment should be carried out to accelerate embryo differentiation and to finish post-mature process.The endogenous inhibitors contained both in seed coat and embryo had significant inhibiting effects on seeds of Brassica pekeinensis and peony it self.The contents and the types of endogenous inhibitors in seed coat were different from those in seed embryo.Extraneous hormone GA3 with a concentration of 300 mg · L-1 could enhance the seed germination rate.In conclusion,the seed dormancy of P.ostii ‘Feng Dan’ was a comprehensive result caused by the im maturity of seeds and the inhibitors.%以凤丹种子(Paeonia ostii)为试验材料,通过对凤丹种子种皮透水性、种胚形态和生活力、种子内源抑制物质活性的测定,研究凤丹种子休眠特性.结果表明:凤丹种皮降低种子吸水速度,但不影响最终吸水率,完整种子浸种56 h种子吸水基本达到饱和,对凤丹种皮进行酸蚀或去皮处理不会提高萌发率,凤丹种皮不是休眠的主要原因;成熟的凤丹种子生活力达95.6%,但种胚发育不完全,其需经过一定时间的层积,种胚才能不断生长分化,完成后熟;种皮和胚乳中含有的萌发抑制物质对白菜种子和自身种子萌发有显著抑制作用,且种皮和胚乳中的

  5. Coordination of genomic structure and transcription by the main bacterial nucleoid-associated protein HU.

    Science.gov (United States)

    Berger, Michael; Farcas, Anca; Geertz, Marcel; Zhelyazkova, Petya; Brix, Klaudia; Travers, Andrew; Muskhelishvili, Georgi

    2010-01-01

    The histone-like protein HU is a highly abundant DNA architectural protein that is involved in compacting the DNA of the bacterial nucleoid and in regulating the main DNA transactions, including gene transcription. However, the coordination of the genomic structure and function by HU is poorly understood. Here, we address this question by comparing transcript patterns and spatial distributions of RNA polymerase in Escherichia coli wild-type and hupA/B mutant cells. We demonstrate that, in mutant cells, upregulated genes are preferentially clustered in a large chromosomal domain comprising the ribosomal RNA operons organized on both sides of OriC. Furthermore, we show that, in parallel to this transcription asymmetry, mutant cells are also impaired in forming the transcription foci-spatially confined aggregations of RNA polymerase molecules transcribing strong ribosomal RNA operons. Our data thus implicate HU in coordinating the global genomic structure and function by regulating the spatial distribution of RNA polymerase in the nucleoid.

  6. Screening active components from Yu-ping-feng-san for regulating initiative key factors in allergic sensitization.

    Science.gov (United States)

    Shen, Dandan; Xie, Xuejian; Zhu, Zhijie; Yu, Xi; Liu, Hailiang; Wang, Huizhu; Fan, Hongwei; Wang, Dawei; Jiang, Guorong; Hong, Min

    2014-01-01

    Yu-ping-feng-san (YPFS) is a Chinese medical formula that is used clinically for allergic diseases and characterized by reducing allergy relapse. Our previous studies demonstrated that YPFS efficiently inhibited T helper 2 cytokines in allergic inflammation. The underlying mechanisms of action of YPFS and its effective components remain unclear. In this study, it was shown that YPFS significantly inhibited production of thymic stromal lymphopoietin (TSLP), an epithelial cell-derived initiative factor in allergic inflammation, in vitro and in vivo. A method of human bronchial epithelial cell (16HBE) binding combined with HPLC-MS (named 16HBE-HPLC-MS) was established to explore potential active components of YPFS. The following five components bound to 16HBE cells: calycosin-7-glucoside, ononin, claycosin, sec-o-glucosylhamaudol and formononetin. Serum from YPFS-treated mice was analyzed and three major components were detected claycosin, formononetin and cimifugin. Among these, claycosin and formononetin were detected by 16HBE-HPLC-MS and in the serum of YPFS-treated mice. Claycosin and formononetin decreased the level of TSLP markedly at the initial stage of allergic inflammation in vivo. Nuclear factor (NF)-κB, a key transcription factor in TSLP production, was also inhibited by claycosin and formononetin, either in terms of transcriptional activation or its nuclear translocation in vitro. Allergic inflammation was reduced by claycosin and formononetin when they are administered only at the initial stage in a murine model of atopic contact dermatitis. Thus, epithelial cell binding combined with HPLC-MS is a valid method for screening active components from complex mixtures of Chinese medicine. It was demonstrated that the compounds screened from YPFS significantly attenuated allergic inflammation probably by reducing TSLP production via regulating NF-κB activation.

  7. Ship Targets Discrimination Algorithm in SAR Images Based on Hu Moment Feature and Texture Feature

    Directory of Open Access Journals (Sweden)

    Liu Lei

    2016-01-01

    Full Text Available To discriminate the ship targets in SAR images, this paper proposed the method based on combination of Hu moment feature and texture feature. Firstly,7 Hu moment features should be extracted, while gray level co-occurrence matrix is then used to extract the features of mean, variance, uniformity, energy, entropy, inertia moment, correlation and differences. Finally the k-neighbour classifier was used to analysis the 15 dimensional feature vectors. The experimental results show that the method of this paper has a good effect.

  8. Neuroprotective effects of the cannabinoid agonist HU210 on retinal degeneration

    OpenAIRE

    Lax Zapata, Pedro; Esquiva Sobrino, Gema; Altavilla, Cesare; Cuenca Navarro, Nicolás

    2014-01-01

    Cannabinoids have been demonstrated to exert neuroprotective effects on different types of neuronal insults. Here we have addressed the therapeutic potential of the synthetic cannabinoid HU210 on photoreceptor degeneration, synaptic connectivity and functional activity of the retina in the transgenic P23H rat, an animal model for autosomal dominant retinitis pigmentosa (RP). In P23H rats administered with HU210 (100 μg/kg, i.p.) from P24 to P90, ERG recordings showed an amelioration of vision...

  9. The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA.

    Science.gov (United States)

    López de Silanes, Isabel; Gorospe, Myriam; Taniguchi, Hiroaki; Abdelmohsen, Kotb; Srikantan, Subramanya; Alaminos, Miguel; Berdasco, María; Urdinguio, Rocío G; Fraga, Mario F; Jacinto, Filipe V; Esteller, Manel

    2009-05-01

    The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression levels increase during tumorigenesis. Here, we present evidence that the expression of DNMT3b is post-transcriptionally regulated by HuR, an RNA-binding protein that stabilizes and/or modulates the translation of target mRNAs. The presence of a putative HuR-recognition motif in the DNMT3b 3'UTR prompted studies to investigate if this transcript associated with HuR. The interaction between HuR and DNMT3b mRNA was studied by immunoprecipitation of endogenous HuR ribonucleoprotein complexes followed by RT-qPCR detection of DNMT3b mRNA, and by in vitro pulldown of biotinylated DNMT3b RNAs followed by western blotting detection of HuR. These studies revealed that binding of HuR stabilized the DNMT3b mRNA and increased DNMT3b expression. Unexpectedly, cisplatin treatment triggered the dissociation of the [HuR-DNMT3b mRNA] complex, in turn promoting DNMT3b mRNA decay, decreasing DNMT3b abundance, and lowering the methylation of repeated sequences and global DNA methylation. In summary, our data identify DNMT3b mRNA as a novel HuR target, present evidence that HuR affects DNMT3b expression levels post-transcriptionally, and reveal the functional consequences of the HuR-regulated DNMT3b upon DNA methylation patterns.

  10. 20世纪中国文学中的 "风水" 叙事%The Feng Shui Narration in the 20 th Century Chinese Literature

    Institute of Scientific and Technical Information of China (English)

    陈旋波

    2015-01-01

    近代以来, 作为中国传统文化一脉的风水观因其缺乏 "真确的知识" 而受到摒弃和批判, 五四以来风水一直受到科学理性、 唯物主义主导的文学思潮的攻伐. 随后, 20世纪中国社会及文化思潮发生了巨大的变迁,风水在文学中的境遇也因时而易, 这不但反映了时代思潮的嬗变, 还纠结着20世纪纷繁复杂的政治、 哲学及美学取向. 尤其是随着90年代中国文化语境的变迁, 风水文化逐渐浮现出其反思现代性的 "复魅" 特质.%In modern times, Feng Shui, which has been inherent to traditional Chinese culture, has been denounced and defamed as lack of scientific and truthful knowledge, and has long been criticized by the rational and materialistic school of literature since the May Fourth Movement. In the 20th century, as the Chinese society and ideology is undergoing dramatic changes, Feng Shui varies from time to time in literature, which reflects not only the transformation of ideology but also the complication of politics, philosophy and aesthetics. Particularly in the 1990s, as the cultural environment in China has greatly changed, the culture of Feng Shui tends to be charged with the nature of "revitalizing the enchantment of the world", i. e. reflections upon modernism.

  11. Hu Shi and the Continuation of the Revolution of1911——Discussing Intellectuals and Social Revolution%Hu Shi and the Continuation of the Revolution of 1911——Discussing Intellectuals and Social Revolution

    Institute of Scientific and Technical Information of China (English)

    Zhu Cheng

    2011-01-01

    Hu Shi is regarded as one of the most important intellectuals after the Revolution of 1911,and he has a far- reaching influence in 20th century.In the perspective of Hu Shi,the contribution and continuation of the Revolution of 1911 has some inspiration for us to analyze the relationship between Chinese intellectuals and social revolution.

  12. 生态建筑学与中国风水学的文化比较%Cultural Comparison of Arcologies and Feng-shui of China

    Institute of Scientific and Technical Information of China (English)

    职建民

    2011-01-01

    This paper introduce the conception of Arcologies and Feng-shui of China.Through cultural comparing with Chinese culture and westen culture,we wish to search a more suitable and effectively design method and norm.%本文对生态建筑学与中国风水学从概念上作一介绍,力图通过在东、西方文化上的比较,得到一个更适用、有效的设计方法和准则。

  13. The Exploration on the Feng Shuey Philosophy of Parametric Architectural Design%探究参数化建筑设计中所蕴藏的风水哲理

    Institute of Scientific and Technical Information of China (English)

    张硕; 王丽

    2014-01-01

    Author studies on feng shuey philosophy of para-metric architectural design, taking galaxy SOHO and CCTV new building as examples to il ustrate that the feng shuey cu-lture which has been passed down for thousand years must have it’s value of spread and that parametric architectural also wil be dif icult to get the inheritance of history out of the feng shuey philosophy.%笔者就参数化建筑中所蕴藏的风水哲理进行探讨,以银河SOHO和央视新大楼为例意在阐明在中国流传了千年之久的风水文化必有其传播价值,并且脱离了风水的参数化建筑也将很难得到历史的传承。

  14. The HU Regulon Is Composed of Genes Responding to Anaerobiosis, Acid Stress, High Osmolarity and SOS Induction

    Science.gov (United States)

    Oberto, Jacques; Nabti, Sabrina; Jooste, Valérie; Mignot, Hervé; Rouviere-Yaniv, Josette

    2009-01-01

    Background The Escherichia coli heterodimeric HU protein is a small DNA-bending protein associated with the bacterial nucleoid. It can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Cells lacking HU grow very poorly and display many phenotypes. Methodology/Principal Findings We analyzed the transcription profile of every Escherichia coli gene in the absence of one or both HU subunits. This genome-wide in silico transcriptomic approach, performed in parallel with in vivo genetic experimentation, defined the HU regulon. This large regulon, which comprises 8% of the genome, is composed of four biologically relevant gene classes whose regulation responds to anaerobiosis, acid stress, high osmolarity, and SOS induction. Conclusions/Significance The regulation a large number of genes encoding enzymes involved in energy metabolism and catabolism pathways by HU explains the highly pleiotropic phenotype of HU-deficient cells. The uniform chromosomal distribution of the many operons regulated by HU strongly suggests that the transcriptional and nucleoid architectural functions of HU constitute two aspects of a unique protein-DNA interaction mechanism. PMID:19194530

  15. The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction.

    Directory of Open Access Journals (Sweden)

    Jacques Oberto

    Full Text Available BACKGROUND: The Escherichia coli heterodimeric HU protein is a small DNA-bending protein associated with the bacterial nucleoid. It can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Cells lacking HU grow very poorly and display many phenotypes. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed the transcription profile of every Escherichia coli gene in the absence of one or both HU subunits. This genome-wide in silico transcriptomic approach, performed in parallel with in vivo genetic experimentation, defined the HU regulon. This large regulon, which comprises 8% of the genome, is composed of four biologically relevant gene classes whose regulation responds to anaerobiosis, acid stress, high osmolarity, and SOS induction. CONCLUSIONS/SIGNIFICANCE: The regulation a large number of genes encoding enzymes involved in energy metabolism and catabolism pathways by HU explains the highly pleiotropic phenotype of HU-deficient cells. The uniform chromosomal distribution of the many operons regulated by HU strongly suggests that the transcriptional and nucleoid architectural functions of HU constitute two aspects of a unique protein-DNA interaction mechanism.

  16. Immunostimulatory effects of natural human interferon-alpha (huIFN-alpha) on carps Cyprinus carpio L.

    Science.gov (United States)

    Watanuki, Hironobu; Chakraborty, Gunimala; Korenaga, Hiroki; Kono, Tomoya; Shivappa, R B; Sakai, Masahiro

    2009-10-15

    Human interferon-alpha (huIFN-alpha) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. However, the immunostimulatory effects of huIFN-alpha in fish remain to be investigated. In the current study, the immune responses of the carp species Cyprinus carpio L. to treatment with huIFN-alpha were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes including interleukin-1beta, tumor necrosis factor-alpha and interleukin 10. Low doses of huIFN-alpha were administered orally once a day for 3 days, and sampling was carried out at 1, 3 and 5 days post-treatment. Our results indicate that a low dose of huIFN-alpha significantly increased phagocytic activity and superoxide anion production in the carp kidney. The huIFN-alpha-treated fish also displayed a significant upregulation in cytokine gene expression. The current study demonstrates the stimulatory effects of huIFN-alpha on the carp immune system and highlights the immunomodulatory role of huIFN-alpha in fish.

  17. First International Workshop on Human Factors in Modeling (HuFaMo 2015)

    DEFF Research Database (Denmark)

    Störrle, Harald; Chaudron, Michel R. V.; Amaral, Vasco

    2015-01-01

    and systematic approaches to assert usability in modeling and confirm the claims of productivity. This workshop creates a space for discussion being a get together of both MDE, Usability, Human Interfaces and the Experimental Software engineering community. HuFaMo expressly focuses on human factors, in order...

  18. Rare Association of Anti-Hu Antibody Positive Paraneoplastic Neurological Syndrome and Transitional Cell Bladder Carcinoma

    Directory of Open Access Journals (Sweden)

    S. Lukacs

    2012-01-01

    Full Text Available Introduction. Paraneoplastic encephalomyelitis (PEM and subacute sensory neuronopathy (SSN are remote effects of cancer, usually associated with small-cell lung carcinoma and positive anti-Hu antibody. We describe the rare association of bladder transitional cell carcinoma (TCC with anti-Hu antibody positivity resulting in this paraneoplastic neurological syndrome. Patient. A 76-year-old female presented with bilateral muscle weakness and paraesthesia of the upper and lower limbs in a length-dependent “glove and stocking” distribution. Central nervous system symptoms included cognitive problems, personality change, and truncal ataxia. Case notes and the literature were reviewed. Result. Autoantibody screening was positive for anti-Hu antibody (recently renamed antineuronal nuclear antibody 1, ANNA-1. The diagnosis of PEM and SSN was supported by MRI and lumbar puncture results. A superficial bladder TCC was demonstrated on CT and subsequently confirmed on histology. No other primary neoplasm was found on full-body imaging. The neurological symptoms were considered to be an antibody-mediated paraneoplastic neurological syndrome and improved after resection of the tumour. Discussion. The association of anti-Hu positive paraneoplastic neurological syndrome and TCC has not been described in the literature previously. We emphasize the need for detailed clinical examination and the importance of a multidisciplinary thought process and encourage further awareness of this rare association.

  19. HuB localizes to polysomes and alters C/EBP-beta expression in 3T3-L1 adipocytes.

    Science.gov (United States)

    Gantt, Kira R; Jain, Renu G; Dudek, Ronald W; Pekala, Phillip H

    2004-01-16

    The RNA binding protein HuB was ectopically expressed in 3T3-L1 preadipocytes and localized primarily to the nucleus, as the cells differentiated HuB redistributed to the cytosol and on analysis localized to the dense polysomes. Electron micrographs confirm association of HuB with the ribosomes in the adipocytes consistent with a proposed role in control of translation and mRNA stability. Examination of the expression of C/EBP-beta in the cells expressing HuB relative to the parental 3T3-L1 adipocytes demonstrated an alteration in the LAP to LIP ratio. The data support a role for endogenous Hu proteins in the differentiation process, potentially affecting the rate of differentiation by controlling the concentration of the dominant negative transcription inhibitor, LIP.

  20. Redox behaviors of the neurotoxic portion in human prion protein, HuPrP(106-126)

    Science.gov (United States)

    Yamamoto, Norifumi; Kuwata, Kazuo

    2010-09-01

    A peptide fragment of human prion protein, HuPrP(106-126), has been reported to mimic the pathological features underlying prion diseases. Although the actual neurotoxic mechanism of HuPrP(106-126) has not been elucidated, several hypotheses has been proposed based on the role for copper. In this study, to understand the toxic function of HuPrP(106-126) from a viewpoint of electrochemical competence, we investigated redox properties of copper ion complexes with four different binding motifs of a model of HuPrP(106-126) based on density functional theory calculations. We found that the HuPrP(106-126)-derived models exhibited diverse redox activities that depended on copper-binding conformations.

  1. Drug Transporter Expression and Activity in Human Hepatoma HuH-7 Cells

    Directory of Open Access Journals (Sweden)

    Elodie Jouan

    2016-12-01

    Full Text Available Human hepatoma cells may represent a valuable alternative to the use of human hepatocytes for studying hepatic drug transporters, which is now a regulatory issue during drug development. In the present work, we have characterized hepatic drug transporter expression, activity and regulation in human hepatoma HuH-7 cells, in order to determine the potential relevance of these cells for drug transport assays. HuH-7 cells displayed notable multidrug resistance-associated protein (MRP activity, presumed to reflect expression of various hepatic MRPs, including MRP2. By contrast, they failed to display functional activities of the uptake transporters sodium taurocholate co-transporting polypeptide (NTCP, organic anion-transporting polypeptides (OATPs and organic cation transporter 1 (OCT1, and of the canalicular transporters P-glycoprotein and breast cancer resistance protein (BCRP. Concomitantly, mRNA expressions of various sinusoidal and canalicular hepatic drug transporters were not detected (NTCP, OATP1B1, organic anion transporter 2 (OAT2, OCT1 and bile salt export pump or were found to be lower (OATP1B3, OATP2B1, multidrug and toxin extrusion protein 1, BCRP and MRP3 in hepatoma HuH-7 cells than those found in human hepatocytes, whereas other transporters such as OAT7, MRP4 and MRP5 were up-regulated. HuH-7 cells additionally exhibited farnesoid X receptor (FXR- and nuclear factor erythroid 2-related factor 2 (Nrf2-related up-regulation of some transporters. Such data indicate that HuH-7 cells, although expressing rather poorly some main hepatic drug transporters, may be useful for investigating interactions of drugs with MRPs, notably MRP2, and for studying FXR- or Nrf2-mediated gene regulation.

  2. Discriminating between HuR and TTP binding sites using the k-spectrum kernel method

    Science.gov (United States)

    Goldberg, Debra S.; Dowell, Robin

    2017-01-01

    Background The RNA binding proteins (RBPs) human antigen R (HuR) and Tristetraprolin (TTP) are known to exhibit competitive binding but have opposing effects on the bound messenger RNA (mRNA). How cells discriminate between the two proteins is an interesting problem. Machine learning approaches, such as support vector machines (SVMs), may be useful in the identification of discriminative features. However, this method has yet to be applied to studies of RNA binding protein motifs. Results Applying the k-spectrum kernel to a support vector machine (SVM), we first verified the published binding sites of both HuR and TTP. Additional feature engineering highlighted the U-rich binding preference of HuR and AU-rich binding preference for TTP. Domain adaptation along with multi-task learning was used to predict the common binding sites. Conclusion The distinction between HuR and TTP binding appears to be subtle content features. HuR prefers strongly U-rich sequences whereas TTP prefers AU-rich as with increasing A content, the sequences are more likely to be bound only by TTP. Our model is consistent with competitive binding of the two proteins, particularly at intermediate AU-balanced sequences. This suggests that fine changes in the A/U balance within a untranslated region (UTR) can alter the binding and subsequent stability of the message. Both feature engineering and domain adaptation emphasized the extent to which these proteins recognize similar general sequence features. This work suggests that the k-spectrum kernel method could be useful when studying RNA binding proteins and domain adaptation techniques such as feature augmentation could be employed particularly when examining RBPs with similar binding preferences. PMID:28333956

  3. Transcriptomic-Wide Discovery of Direct and Indirect HuR RNA Targets in Activated CD4+ T Cells.

    Directory of Open Access Journals (Sweden)

    Patsharaporn Techasintana

    Full Text Available Due to poor correlation between steady state mRNA levels and protein product, purely transcriptomic profiling methods may miss genes posttranscriptionally regulated by RNA binding proteins (RBPs and microRNAs (miRNAs. RNA immunoprecipitation (RIP methods developed to identify in vivo targets of RBPs have greatly elucidated those mRNAs which may be regulated via transcript stability and translation. The RBP HuR (ELAVL1 and family members are major stabilizers of mRNA. Many labs have identified HuR mRNA targets; however, many of these analyses have been performed in cell lines and oftentimes are not independent biological replicates. Little is known about how HuR target mRNAs behave in conditional knock-out models. In the present work, we performed HuR RIP-Seq and RNA-Seq to investigate HuR direct and indirect targets using a novel conditional knock-out model of HuR genetic ablation during CD4+ T activation and Th2 differentiation. Using independent biological replicates, we generated a high coverage RIP-Seq data set (>160 million reads that was analyzed using bioinformatics methods specifically designed to find direct mRNA targets in RIP-Seq data. Simultaneously, another set of independent biological replicates were sequenced by RNA-Seq (>425 million reads to identify indirect HuR targets. These direct and indirect targets were combined to determine canonical pathways in CD4+ T cell activation and differentiation for which HuR plays an important role. We show that HuR may regulate genes in multiple canonical pathways involved in T cell activation especially the CD28 family signaling pathway. These data provide insights into potential HuR-regulated genes during T cell activation and immune mechanisms.

  4. Intestinal epithelial HuR modulates distinct pathways of proliferation and apoptosis and attenuates small intestinal and colonic tumor development.

    Science.gov (United States)

    Giammanco, Antonina; Blanc, Valerie; Montenegro, Grace; Klos, Coen; Xie, Yan; Kennedy, Susan; Luo, Jianyang; Chang, Sung-Hee; Hla, Timothy; Nalbantoglu, Ilke; Dharmarajan, Sekhar; Davidson, Nicholas O

    2014-09-15

    HuR is a ubiquitous nucleocytoplasmic RNA-binding protein that exerts pleiotropic effects on cell growth and tumorigenesis. In this study, we explored the impact of conditional, tissue-specific genetic deletion of HuR on intestinal growth and tumorigenesis in mice. Mice lacking intestinal expression of HuR (Hur (IKO) mice) displayed reduced levels of cell proliferation in the small intestine and increased sensitivity to doxorubicin-induced acute intestinal injury, as evidenced by decreased villus height and a compensatory shift in proliferating cells. In the context of Apc(min/+) mice, a transgenic model of intestinal tumorigenesis, intestinal deletion of the HuR gene caused a three-fold decrease in tumor burden characterized by reduced proliferation, increased apoptosis, and decreased expression of transcripts encoding antiapoptotic HuR target RNAs. Similarly, Hur(IKO) mice subjected to an inflammatory colon carcinogenesis protocol [azoxymethane and dextran sodium sulfate (AOM-DSS) administration] exhibited a two-fold decrease in tumor burden. Hur(IKO) mice showed no change in ileal Asbt expression, fecal bile acid excretion, or enterohepatic pool size that might explain the phenotype. Moreover, none of the HuR targets identified in Apc(min/+)Hur(IKO) were altered in AOM-DSS-treated Hur(IKO) mice, the latter of which exhibited increased apoptosis of colonic epithelial cells, where elevation of a unique set of HuR-targeted proapoptotic factors was documented. Taken together, our results promote the concept of epithelial HuR as a contextual modifier of proapoptotic gene expression in intestinal cancers, acting independently of bile acid metabolism to promote cancer. In the small intestine, epithelial HuR promotes expression of prosurvival transcripts that support Wnt-dependent tumorigenesis, whereas in the large intestine epithelial HuR indirectly downregulates certain proapoptotic RNAs to attenuate colitis-associated cancer. Cancer Res; 74(18); 5322-35. ©2014 AACR.

  5. 也说《飞鸟集》冯唐译本%Comment on Feng Tang’s Translation of Stray Birds

    Institute of Scientific and Technical Information of China (English)

    胡俊麟

    2016-01-01

    《飞鸟集》冯唐译本,多数读者持有激烈的抵制意见,也有读者持较包容的态度。浙江文艺出版社宣布下架并召回该书,将争论引向高潮。冯唐译本引发争议,至少包含三个方面的问题:在翻译历史背景下有失规范,粗暴挣脱中国现代文学史的影响力,未能展现《飞鸟集》深刻的精神内核等。%Although some readers hold a more tolerant attitude, Stray Birds, Feng Tang’s translated version, has drawn fierce resistance from the majority of readers. The announcement, which Zhejiang Literature and Art Publishing House made to take the book off the shelves and recall the books sold, led the controversy to a climax. Feng Tang’s translation doesn’t consist with the norms which are formed in translation history and it rudely breaks away from the influence of Chinese modern literature. Meanwhile it failed to present the deep spiritual core of Stray Birds. In part, these are the reasons triggering the controversy.

  6. 冯友兰代理清华校务的贡献%On the Contributions of Feng Youlan as Acting President of Qinghua University

    Institute of Scientific and Technical Information of China (English)

    王仁宇

    2012-01-01

    In the development history of Qinghua University,Feng Youlan was elected as Acting President of Qinghua University during the period between the vocation of presidency of Qinghua by Luo Jialun and the assumption of that post by Mei Yiqi.Feng Youlan had made huge contributions to the stability and development of Qinghua University under extreme historical circumstances by leading the university council in maintaining the sound operation of university affairs,resisting the interference of warlords,standing against disruptive attempts at obstructing the tradition of "rule of professors" by Wu Nanxuan,and actively participating in and organizing activities for the War of Resistance Against Japan.%在清华大学的发展史上,从罗家伦辞职到梅贻琦到任一年多的时间里,冯友兰被推举代理校务。他领导校务委员会,在维持学校正常运作的同时,抵制军阀势力对清华的干预,反对吴南轩对教授治校传统的破坏,积极参加并组织抗日活动,为清华大学在特殊时期的稳定和发展作出了重大贡献。

  7. Carrying forward the Spirit of Lei Feng and the Innovation of Social Management%弘扬雷锋精神与社会管理创新

    Institute of Scientific and Technical Information of China (English)

    邓智平

    2012-01-01

    在现阶段社会管理创新中。很少跳出“社会”论社会管理,忽视了社会管理与政治、经济、文化等其他领域的密切关系。雷锋精神作为时代精神的重要内容,对社会管理创新具有重要价值。在政府职能转变、调动社会资源增进民生福祉、激发公众参与、社会工作和志愿服务等方面雷锋精神有着广泛的应用。%At the present stage, the innovation of social management still stays in the social management of "social theory", ignores the close relationship between the social management and the political, economic, cultural and other fields. Lei Feng spirit, as the important content of the times spirit, has the important value to the innovate the social management. The spirit of Lei Feng has a wide range of applications to transit the government functions, mobilize community resources, improve people's livelihood, stimulate public participation, social work and volunteer services and other aspects.

  8. 胞质HuR在卵巢上皮性癌的表达及意义%The expression and significance of cytoplasmic HuR in epithelial ovarian carcinoma

    Institute of Scientific and Technical Information of China (English)

    齐玉明; 石彬; 郭皓; 成艳梅

    2011-01-01

    Objective To investigate the correlation between the expression of HuR in epithelial ovarian cancer and clinicopathological parameters in order to explore the factors that affect the prognosis of patients with epithelial ovarian carcinoma. Methods The expressions of cytoplasmic and nuclear HuR in normal ovary tissues,borderline tissue of ovary tumor and epithelial ovarian carcinoma were detected by SP immunohistochemical technique and RT-PCR. The relationship between cytoplasmic HuR expression and prognostic factors was analyzed.Results The expressions of cytoplasmic and nuclear HuR in epithelial ovarian cancer tissue were significantly higher than those in borderline tissue of epithelial ovarian tumor and normal ovarian tissues,however,there was no significant difference between the borderline tissue of epithelial ovarian tumor and normal ovarian tissue ( P >0.05). The statistical analysis showed that the positive expression rate of cytoplasmic HuR in epithelial ovarian carcinoma was closely correlated with histological grade and FIGO stage, but the positive expression rate of nuclear HuR was not correlated with the clinicopathological parameters. Conclusion The over-expression of HuR may play an important role in the growth and progress of epithelial ovarian carcinoma. To detect the expression of cytoplasmic HuR in epithelial ovarian carcinoma may predict the characteristics of oncobiology behaviour. The expression of cytoplasmic HuR may act as an independent prognostic indicator.%目的 检测HuR在卵巢上皮性癌中的表达及与临床病理参数的关系,寻求卵巢癌的预后因素.方法 采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法和逆转录RT-PCR技术,检测胞质和胞核HuR在正常、交界性、恶性卵巢组织中的表达;分析胞质HuR的表达与预后的关系.结果 胞质和胞核HuR在恶性卵巢肿瘤组织的表达明显高于交界性卵巢肿瘤和正常卵巢组织,胞质和胞核HuR在交界性

  9. Reversible HuR-microRNA binding controls extracellular export of miR-122 and augments stress response.

    Science.gov (United States)

    Mukherjee, Kamalika; Ghoshal, Bartika; Ghosh, Souvik; Chakrabarty, Yogaditya; Shwetha, Shivaprasad; Das, Saumitra; Bhattacharyya, Suvendra N

    2016-08-01

    microRNAs (miRNAs), the tiny but stable regulatory RNAs in metazoan cells, can undergo selective turnover in presence of specific internal and external cues to control cellular response against the changing environment. We have observed reduction in cellular miR-122 content, due to their accelerated extracellular export in human hepatic cells starved for small metabolites including amino acids. In this context, a new role of human ELAV protein HuR has been identified. HuR, a negative regulator of miRNA function, accelerates extracellular vesicle (EV)-mediated export of miRNAs in human cells. In stressed cells, HuR replaces miRNPs from target messages and is both necessary and sufficient for the extracellular export of corresponding miRNAs. HuR could reversibly bind miRNAs to replace them from Ago2 and subsequently itself gets freed from bound miRNAs upon ubiquitination. The ubiquitinated form of HuR is predominantly associated with multivesicular bodies (MVB) where HuR-unbound miRNAs also reside. These MVB-associated pool of miRNAs get exported out via EVs thereby delimiting cellular miR-122 level during starvation. Therefore, by modulating extracellular export of miR-122, HuR could control stress response in starved human hepatic cells.

  10. SU-E-T-470: Importance of HU-Mass Density Calibration Technique in Proton Pencil Beam Dose Calculation

    Energy Technology Data Exchange (ETDEWEB)

    Penfold, S; Miller, A [University of Adelaide, Adelaide, SA (Australia)

    2015-06-15

    Purpose: Stoichiometric calibration of Hounsfield Units (HUs) for conversion to proton relative stopping powers (RStPs) is vital for accurate dose calculation in proton therapy. However proton dose distributions are not only dependent on RStP, but also on relative scattering power (RScP) of patient tissues. RScP is approximated from material density but a stoichiometric calibration of HU-density tables is commonly neglected. The purpose of this work was to quantify the difference in calculated dose of a commercial TPS when using HU-density tables based on tissue substitute materials and stoichiometric calibrated ICRU tissues. Methods: Two HU-density calibration tables were generated based on scans of the CIRS electron density phantom. The first table was based directly on measured HU and manufacturer quoted density of tissue substitute materials. The second was based on the same CT scan of the CIRS phantom followed by a stoichiometric calibration of ICRU44 tissue materials. The research version of Pinnacle{sup 3} proton therapy was used to compute dose in a patient CT data set utilizing both HU-density tables. Results: The two HU-density tables showed significant differences for bone tissues; the difference increasing with increasing HU. Differences in density calibration table translated to a difference in calculated RScP of −2.5% for ICRU skeletal muscle and 9.2% for ICRU femur. Dose-volume histogram analysis of a parallel opposed proton therapy prostate plan showed that the difference in calculated dose was negligible when using the two different HU-density calibration tables. Conclusion: The impact of HU-density calibration technique on proton therapy dose calculation was assessed. While differences were found in the calculated RScP of bony tissues, the difference in dose distribution for realistic treatment scenarios was found to be insignificant.

  11. Altered regulation of ELAVL1/HuR in HLA-B27-expressing U937 monocytic cells.

    Directory of Open Access Journals (Sweden)

    Anna S Sahlberg

    Full Text Available OBJECTIVE: To investigate the role of HLA-B27 expression in the regulation of RNA binding protein (RBP Embryonic Lethal Abnormal Vision (ELAV L1/Human antigen R (HuR expression in Salmonella-infected or LPS-stimulated human monocytic cells, since HuR is a critical regulator of the post-transcriptional fate of many genes (e.g. TNFα important in inflammatory response. METHODS: U937 monocytic cells were stably transfected with pSV2neo resistant vector (mock, wild type HLA-B27, or mutated HLA-B27 with amino acid substitutions in the B pocket. Cells were differentiated, infected with Salmonella enteritidis or stimulated with lipopolysaccharide. The expression levels of HuR protein and cleavage products (CP1 and CP2 were detected by Western blotting and flow cytometry. Specific inhibitors were used to study the role of PKR and p38 in HuR expression and generation of CPs. TNFα and IL-10 secretion after p38 and PKR inhibition were measured by ELISA. RESULTS: Full length HuR is overexpressed and HuR cleavage is disturbed in U937 monocytic cells expressing HLA-B27 heavy chains (HC. Increased full length HuR expression, disturbed cleavage and reduced dependence on PKR after infection correlate with the expression of glutamic acid 45 in the B pocket that is linked to the misfolding of HLA-B27. CONCLUSION: Results show that the expression of HLA-B27 HCs modulates the intracellular environment of U937 monocyte/macrophages by altering HuR regulation. This phenomenon is at least partly dependent on the misfolding feature of the B27 molecule. Since HuR is an important regulator of multiple genes involved in inflammatory response observations offer an explanation how HLA-B27 may modulate inflammatory response.

  12. A Study on the Belief and Theoretical Form of Feng shui in the Han Dynasty from Tai ping Ching%《太平经》所见汉代风水观念及理论形态

    Institute of Scientific and Technical Information of China (English)

    张齐明

    2011-01-01

    The concept of "Qi tu" good or ill luck and "live ghost and spirit"(a theory of the Feng shui) proposed in Tai ping Ching,written in the late Eastern Han Dynasty,is the reflection of rapid expansion of Feng shui belief in the Han Dynasty.It also marks the theoretical form of Feng shui funeral.The text suggests that Taoism transforms and absorbs Feng shui in its religious system,not to meet the belief of people,but rather to guide and transform it into Taoism belief system.%成书于东汉中晚期的《太平经》所提出的"起土"吉凶观念及"魂神还养"的丧葬风水理论,是汉代社会风水信仰急剧膨胀的反映,它也标志着丧葬风水术理论形态基本形成。《太平经》中风水文献表明,道教对风水术的吸收与改造是在宗教体系之内进行的,并不是对民众信仰的无限制迎合,而是加以引导和转化,从而纳入其信仰体系之中。

  13. Central hypoventilation as the presenting symptom in Hu associated paraneoplastic encephalomyelitis.

    Science.gov (United States)

    Gómez-Choco, Manuel J; Zarranz, Juan J; Saiz, Albert; Forcadas, María I; Graus, Francesc

    2007-10-01

    Central hypoventilation is usually caused by ischaemic or neoplastic lesions of the medulla and upper cervical spinal cord. An autoimmune disorder is not usually considered in the differential diagnosis of this syndrome. We retrospectively identified 14 patients from our database of 202 patients with Hu antibodies who presented with brainstem symptoms. Three were admitted to hospital because of central hypoventilation. All underwent intubation and mechanical ventilation. They could breathe properly while they were awake but suffered deep apnoeas during sleep. Two died, but one is still alive requiring ventilatory assistance during sleep. Autopsy was performed in one of the patients which showed severe inflammatory infiltrates and neuronal loss in the medulla. All patients had normal brain imaging studies and the cause of central hypoventilation was an unsolved problem until Hu antibodies were determined.

  14. Discuss on the Idea of FENG Gui-fen's Society Integration%冯桂芬社会整合思想探析

    Institute of Scientific and Technical Information of China (English)

    刘庆艳; 王蕊; 花琦; 许南海

    2011-01-01

    Discuss on the idea of FENG gui-fen's society integration from the sociological angle.In the case of internal and external problems,how to solve social problems,make the society become an orderly and normal track,so as to achieve to be able to rule the people internally and against enemy externally,FENG gui-fen proposed the idea of society integration that "Chinese old ethics and norms are the most fundamental,at the same time we need to study the prosperous methods of the other countries which are prosperous at that time.In order to describe the idea of FENG gui-fen's society Integrtion,this article from three aspects that "strengthening of the patriarchal organization and the patriarchal hierarchy","imitation the sages' method of three generations' to consolidate the state";"learning western skills to make the country become rich and strong".So that the idea of the background,content and meaning show up clearly.%在内忧外患的情况下,怎样解决社会问题,使社会形成一个有序、正常发展的轨道,从而达到内能治民、外能敌国的目标,冯桂芬提出了"以中国之伦常名教为原本,辅以诸国富强之术"的社会整合思想。文章从加强宗法组织与宗法等级制、仿"三代圣人之法"以固国本、学西洋长技以强国富国三个方面对冯桂芬的社会整合思想进行阐述,展现了这一思想提出的背景、内容及意义。

  15. 试论丰子恺的工艺美术思想%On the Ideological Systems of Arts and Crafts of Feng Zikai

    Institute of Scientific and Technical Information of China (English)

    崔晓彦

    2016-01-01

    丰子恺工艺美术思想的主要内容为工艺美术本质观、工艺美术伦理观和工艺美术审美观。其思想当是对丰子恺工艺美术创作的表现形式、艺术理论中与工艺美术问题相关的观点、见解甚至生平行事的提炼和归纳,而非出自丰子恺自己有意识地整理与归纳,因此也就决定了该思想系统性和深刻性的不足,影响了个人特色和时代精神的充分显现。瑕不掩瑜,丰子恺有关工艺美术的美育功能、对“人”的痕迹的重视和“中西融合”的价值观等对当代艺术设计依然具有重要的启迪作用。%The ideological system of arts and crafts of Feng Zikai contains three aspects, i.e. arts and crafts essence view, arts and crafts ethical view, and arts and crafts aesthetical view. It is from the summary of the form of expression in his arts and crafts works, relevant content of arts and crafts in his arts theory, his essay, correspondence, life chronicle, and not concluded by Feng Zikai himself consciously. Therefore, the ideological system of arts and crafts of Feng Zikai is insufifcient in systemic and depth. The defects cannot obscure the virtues it is well revelatory for the development of modern art design in these respects, i.e. aesthetic education function of arts and crafts, high attention of traces of people in creation of art, and the fusion of west and east arts and crafts.

  16. Cotton Science Research of Feng Zefang and His Main Contribution%冯泽芳的棉作科学研究及其主要贡献

    Institute of Scientific and Technical Information of China (English)

    曾玉珊

    2012-01-01

    冯泽芳是中国现代棉作科学的主要奠基人。他首次列出完整的中棉分类系统表,奠定了中国亚洲棉分类的基础。冯泽芳是我国用孟德尔定律对亚洲棉性状进行遗传研究的先导,较早从事棉花种间杂交及后代的遗传学和细胞学研究,研究结论处于同类研究的先进水平。冯泽芳对木棉进行科学鉴定,肯定其经济价值并大力推广。冯泽芳主持的棉花区试在主要农作物的区域试验中首开先河,为20世纪30年代中叶以后推广斯字棉与德字棉提供了科学依据。冯泽芳关于棉区划分及棉纺工业布局的科学思想成为我国发展商品棉基地,重新进行棉纺业布局调整的重要根据。%Feng Zefang was main founder of modem cotton science in China.Feng was the first in the history of China to list the complete classification form of Chinese cotton, which established the foundation of the classification of Chinese Asiatic cotton. He also pioneered genetic research on Asiatic cotton by Mendel's Law in China, and was among the first to work on the cytology and genetics research of their offspring. Feng' s conclusion reached an advanced level in similar research field that captured the botany world's attention from all over the world. He made a scientific verification for tree-cotton, affirmed it economic value and made a lot of efforts to release it. His cotton-planting region experiment imitated the region experiment of staple crops,which provided the scientific evidence for the release of Delfos and Stonevile. His scientific thoughts about the divi- sion of cotton-planting regions and the layout of cotton spinning industry lay an important foundation for the development commodity cotton bases and the readjustment of the layout of cotton spinning industry in China.

  17. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells

    Directory of Open Access Journals (Sweden)

    Xiao-Hong Li

    2013-01-01

    Full Text Available Calcium signals in hepatocytes control cell growth, proliferation, and death. Members of the transient receptor potential (TRP cation channel superfamily are candidate calcium influx channels. NFκB activation strictly depends on calcium influx and often induces antiapoptotic genes favouring cell survival. Previously, we reported that S-[6]-gingerol is an efficacious agonist of the transient receptor potential cation channel subfamily V member 1 (TRPV1 in neurones. In this study, we tested the effect of S-[6]-gingerol on HuH-7 cells using the Fluo-4 calcium assay, RT-qPCR, transient cell transfection, and luciferase measurements. We found that S-[6]-gingerol induced a transient rise in [Ca2+]i in HuH-7 cells. The increase in [Ca2+]i induced by S-[6]-gingerol was abolished by preincubation with EGTA and was also inhibited by the TRPV1 channel antagonist capsazepine. Expression of TRPV1 in HuH-7 cells was confirmed by mRNA analysis as well as a test for increase of [Ca2+]i by TRPV1 agonist capsaicin and its inhibition by capsazepine. We found that S-[6]-gingerol induced rapid NFκB activation through TRPV1 in HuH-7 cells. Furthermore, S-[6]-gingerol-induced NFκB activation was dependent on the calcium gradient and TRPV1. The rapid NFκB activation by S-[6]-gingerol was associated with an increase in mRNA levels of NFκB-target genes: cIAP-2, XIAP, and Bcl-2 that encode antiapoptotic proteins.

  18. Effects of animal class and genotype on beef muscle nanostructure, pHu, colour and tenderness

    Directory of Open Access Journals (Sweden)

    Amanda Y. Chulayo

    2016-07-01

    Full Text Available The objective of the study was to determine the effects of animal class and genotype of cattle on Muscularis longissimus thoracis et lumborum (LTL nanostructure, ultimate pH (pHu, colour and tenderness of beef. We found significant positive relationships between distance travelled (DT and meat temperature (Tm (p"less than"0.01; lairage duration (LDhr and lightness of colour (L* (p"less than"0.01; ambient temperature (Ta and L* (p"less than"0.05 and LDhr and yellowness (b* (p"less than"0.05 of beef from Bonsmara cattle. Positive linear relationships were observed between DT and Tm (p"less than"0.05 and DT and L* (p"less than"0.01 of the non-descript cattle. There were no significant relationships between pre-slaughter stress and other beef quality parameters (pHu, Warner– Bratzler shear force [WBSF], redness [a*] and b* of Bonsmara, Nguni and non-descript cattle. Muscle fibres differed among class and genotype and had an effect on meat quality. The Bonsmara, non-descript and Nguni cows and heifers had visible skeletal muscle fibres which were thin and long, promising improved tenderness of beef. Genotype and class had significant effects on meat quality parameters (Tm, pHu, L*, a*, b* and WBSF. The first important principal components as they appeared from the analysis were pHu, Tm, L*, a*, b* and WBSF. Therefore, animal class did not affect the nanostructure of beef; instead, meat tenderness was enhanced by the longer and visible muscle fibres. Nguni cattle produced meat of superior quality to that of the Bonsmara and the non-descript cattle.

  19. The outcome of PIE *#Hi- and *#Hu- in Germanic

    DEFF Research Database (Denmark)

    Hansen, Bjarne Simmelkjær Sandgaard

    2015-01-01

    It has been established with a great amount of certainty that PIE *#Hu- > Gr. #Vὐ- and that PIE *#Hi- > Gr. #ἰ-. It still remains to be demonstrated what happens in other Western Indo-European branches, including Germanic. In this article , I reject the statement by Ringe (1988: 433) that PIE *#H...... tentatively propose that PG *#au- > PG *#u- when followed by a labial consonant; a development partially paralleled in Greek and in English....

  20. Sustainable groundwater development and management in the Quaternary Hang-Jia-Hu Plain, China

    Institute of Scientific and Technical Information of China (English)

    朱琰

    2002-01-01

    Based on the results of study on regional water supply system, water quality assessments, Quaternary aquifers investigation, and correlation analysis of groundwater depression resulting from land subsidence in the Hang-Jia-Hu Quaternary Plain, this paper presents the groundwater resources policy and sustainable management methods suitable for this area. Suggestions for controlling land subsidence by implementation of wise groundwater policy and management measures are also given.

  1. The outcome of PIE *#Hi- and *#Hu- in Germanic

    DEFF Research Database (Denmark)

    Hansen, Bjarne Simmelkjær Sandgaard

    2015-01-01

    It has been established with a great amount of certainty that PIE *#Hu- > Gr. #Vὐ- and that PIE *#Hi- > Gr. #ἰ-. It still remains to be demonstrated what happens in other Western Indo-European branches, including Germanic. In this article , I reject the statement by Ringe (1988: 433) that PIE *#H...... tentatively propose that PG *#au- > PG *#u- when followed by a labial consonant; a development partially paralleled in Greek and in English....

  2. Screening candidate microRNAs (miRNAs) in different lambskin hair follicles in Hu sheep.

    Science.gov (United States)

    Gao, Wen; Sun, Wei; Yin, Jinfeng; Lv, Xiaoyang; Bao, Jianjun; Yu, Jiarui; Wang, Lihong; Jin, Chengyan; Hu, Liang

    2017-01-01

    Hu sheep lambskin is a unique white lambskin from China that exhibits three types of flower patterns, including small waves, medium waves, and large waves, with small waves considered the best quality. However, our understanding of the molecular mechanism underlying flower pattern formation in Hu sheep lambskin is limited. The aim of the present study was to further explore the relevance between candidate microRNAs (miRNAs) and developmental characteristics of hair follicles and screen miRNAs for later functional validation. Herein, we employed Illumina Hiseq 2500 to identify differentially expressed miRNAs in hair follicles of different flower patterns with small, medium, and large waves to construct a comprehensive sequence database on the mechanism of hair follicle development. Paraffin sections of lambskin tissue were prepared to assess the structure of different hair follicles. Expression levels of candidate miRNAs in different flower patterns were analyzed by relative quantitation using real-time PCR, combined with histological observation and micro-observation technologies, and the correlation between expression levels of candidate miRNAs and histological properties of hair follicles was analyzed by using SPSS 17.0. A total of 522 differentially expressed miRNAs were identified, and RNA-seq analysis detected 7,266 target genes in different groups of flower patterns. Gene ontological analysis indicated these target genes were mainly involved in cell proliferation, differentiation, growth, apoptosis, and ion transport, and 14 miRNAs, including miR-143, miR-10a, and let-7 were screened as candidate miRNAs in Hu sheep hair follicle growth and development. In the same field of vision, variance analysis showed that the number of secondary follicles in small waves was significantly larger than that in large and medium waves (Phair follicles, highly significant differences in miRNA-143 expression levels between large and small waves were observed (Phair follicle

  3. Downregulation of β-Adrenoceptors in Isoproterenol-Induced Cardiac Remodeling through HuR.

    Directory of Open Access Journals (Sweden)

    Qian Yin

    Full Text Available β-adrenergic receptors (β-ARs play an important role in cardiac remodeling, which is the key pathological process in various heart diseases and leads to heart failure. However, the regulation of β-AR expression in remodeling hearts is still unclear. This study aims to clarify the possible mechanisms underlying the regulation of β1- and β2-AR expression in cardiac remodeling. The rat model of cardiac remodeling was established by subcutaneous injection of isoproterenol(ISO at the dose of 0.25 mg·kg(-1·d(-1 for 7 days. We found that the expression of β1- and β2-ARs decreased in the remodeling heart. The mechanisms may include the inhibition of DNA transcription and the increase of mRNA degradation. cAMP-response element binding protein(CREB is a well-known transcription factor of β-AR. However, the expression and activation of CREB was not changed in the remodeling heart. Further, human Antigen-R (HuR, a RNA binding protein, which binds to the 3'-untranslated region of the β-AR mRNA and promotes RNA degradation, was increased in the remodeling model. And in vitro, HuR deficiency reversed the reduction of β-AR mRNA induced by ISO. Therefore, the present findings indicate that HuR, but not CREB, is responsible for the reduction of β-AR expression in ISO induced cardiac remodeling.

  4. Intraperirhinal cortex administration of the synthetic cannabinoid, HU210, disrupts object recognition memory in rats.

    Science.gov (United States)

    Sticht, Martin A; Jacklin, Derek L; Mechoulam, Raphael; Parker, Linda A; Winters, Boyer D

    2015-03-25

    Cannabinoids disrupt learning and memory in human and nonhuman participants. Object recognition memory, which is particularly susceptible to the impairing effects of cannabinoids, relies critically on the perirhinal cortex (PRh); however, to date, the effects of cannabinoids within PRh have not been assessed. In the present study, we evaluated the effects of localized administration of the synthetic cannabinoid, HU210 (0.01, 1.0 μg/hemisphere), into PRh on spontaneous object recognition in Long-Evans rats. Animals received intra-PRh infusions of HU210 before the sample phase, and object recognition memory was assessed at various delays in a subsequent retention test. We found that presample intra-PRh HU210 dose dependently (1.0 μg but not 0.01 μg) interfered with spontaneous object recognition performance, exerting an apparently more pronounced effect when memory demands were increased. These novel findings show that cannabinoid agonists in PRh disrupt object recognition memory.

  5. 论胡锦涛的人才观%On HuJintao' s View of Talent

    Institute of Scientific and Technical Information of China (English)

    于伟峰; 黄静

    2012-01-01

    胡锦涛的人才观主要体现在:树立人才资源为第一资源、人人皆可成才和以人为本的观念。胡锦涛的人才观的核心内容与前三代领导集体人才观的核心内容既一眯相承又有其独特的创新点,他首次提出了党管人才的原则和将人才观与以人为本的发展观相结合的原则。%Hu Jintao' s view of talents is embodied in 1 ) his view that human resources are paramount to all other resources; 2) his belief that everyone can develop into a man of caliber; and 3) his people-oriented approach to development. The kernel of Hu Jintao' s view of talent is imbued with the same spirit reflected in the general out- look on talent of previous three generations of central collective leadership of the CPC, while boasting unique inno- vations. Hu first puts forward the principle of managing talents by the party and the principle of combining the sci- entific view of talent with the people-oriented approach to development.

  6. Anti-solar differential rotation on the active sub-giant HU Virginis

    CERN Document Server

    Harutyunyan, G; Künstler, A; Carroll, T A; Weber, M

    2016-01-01

    Measuring surface differential rotation (DR) on different types of stars is important when characterizing the underlying stellar dynamo. It has been suggested that anti-solar DR laws can occur when strong meridional flows exist. We aim to investigate the differential surface rotation on the primary star of the RS CVn binary HU Vir by tracking its starspot distribution as a function of time. We also aim to recompute and update the values for several system parameters of the triple system HU Vir (close and wide orbits). Time-series high-resolution spectroscopy for four continuous months was obtained with the 1.2-m robotic STELLA telescope. Nine consecutive Doppler images were reconstructed from these data, using our line-profile inversion code iMap. An image cross-correlation method was applied to derive the surface differential-rotation law for HU Vir. New orbital elements for the close and the wide orbits were computed using our new STELLA radial velocities (RVs) combined with the RV data available in the lit...

  7. CTXφ Replication Depends on the Histone-Like HU Protein and the UvrD Helicase.

    Directory of Open Access Journals (Sweden)

    Eriel Martínez

    2015-05-01

    Full Text Available The Vibrio cholerae bacterium is the agent of cholera. The capacity to produce the cholera toxin, which is responsible for the deadly diarrhea associated with cholera epidemics, is encoded in the genome of a filamentous phage, CTXφ. Rolling-circle replication (RCR is central to the life cycle of CTXφ because amplification of the phage genome permits its efficient integration into the genome and its packaging into new viral particles. A single phage-encoded HUH endonuclease initiates RCR of the proto-typical filamentous phages of enterobacteriaceae by introducing a nick at a specific position of the double stranded DNA form of the phage genome. The rest of the process is driven by host factors that are either essential or crucial for the replication of the host genome, such as the Rep SF1 helicase. In contrast, we show here that the histone-like HU protein of V. cholerae is necessary for the introduction of a nick by the HUH endonuclease of CTXφ. We further show that CTXφ RCR depends on a SF1 helicase normally implicated in DNA repair, UvrD, rather than Rep. In addition to CTXφ, we show that VGJφ, a representative member of a second family of vibrio integrative filamentous phages, requires UvrD and HU for RCR while TLCφ, a satellite phage, depends on Rep and is independent from HU.

  8. Cannabinoids & Stress: impact of HU-210 on behavioral tests of anxiety in acutely stressed mice.

    Science.gov (United States)

    Kinden, Renee; Zhang, Xia

    2015-05-01

    Anxiety disorders are one of the most prevalent classes of mental disorders affecting the general population, but current treatment strategies are restricted by their limited efficacy and side effect profiles. Although the cannabinoid system is speculated to be a key player in the modulation of stress responses and emotionality, the vast majority of current research initiatives had not incorporated stress exposure into their experimental designs. This study was the first to investigate the impact of exogenous cannabinoid administration in an acutely stressed mouse model, where CD1 mice were pre-treated with HU-210, a potent CB1R agonist, prior to acute stress exposure and subsequent behavioral testing. Exogenous cannabinoid administration induced distinct behavioral phenotypes in stressed and unstressed mice. While low doses of HU-210 were anxiolytic in unstressed subjects, this effect was abolished when mice were exposed to an acute stressor. The administration of higher HU-210 doses in combination with acute stress exposure led to severe locomotor deficits that were not previously observed at the same dose in unstressed subjects. These findings suggest that exogenous cannabinoids and acute stress act synergistically in an anxiogenic manner. This study underlies the importance of including stress exposure into future anxiety-cannabinoid research due to the differential impact of cannabinoid administration on stressed and unstressed subjects.

  9. Clinical study on intravitreal injection of cannabinoid HU-211 for optic nerve damage in glaucoma rats

    Directory of Open Access Journals (Sweden)

    Hui-Feng Liu

    2014-09-01

    Full Text Available AIM: To evaluate the protective effects of intravitreal injection of cannabinoid HU-211 for optic nerve in glaucoma rats, providing the experimental evidence in the treatment of glaucoma optic nerve injury.METHODS: Glaucoma rats(18 eyeswere modeled by electric coagulation sclera surface vein and randomized into 3 groups, group A received intravitreal injection of 1mg/0.1mL cannabinoid HU-211 every other day respectively; group B was given intravitreal injection of 0.1mL water every other day, group C was high intraocular pressure(IOPgroup, 6 eyes were randomly selected for blank control group(group D. IOP was observed every day. The rats were sacrificed after treatment 4wk, froze retina section, HE stain. The density fluctuation of retinal ganglion cell(RGCneurons assessment the optic nerve of rat model with chronic high IOP glaucoma were measured.RESULTS: The apoptosis and damage degree of RGC in group B was obviously higher than that in group A, with statistically significant difference(PP >0.05.CONCLUSION: Intravitreal injection of cannabinoid HU-211 shows obvious protective effect on optic nerve in glaucom rat models.

  10. The cytoskeletal inhibitors latrunculin A and blebbistatin exert antitumorigenic properties in human hepatocellular carcinoma cells by interfering with intracellular HuR trafficking

    Energy Technology Data Exchange (ETDEWEB)

    Doller, Anke; Badawi, Amel [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Schmid, Tobias; Brauß, Thilo [Institut für Biochemie I (Pathobiochemie), Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Pleli, Thomas [Medizinische Klinik 1, Schwerpunkt Gastroenterologie und Hepatologie, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Meyer zu Heringdorf, Dagmar [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Piiper, Albrecht [Medizinische Klinik 1, Schwerpunkt Gastroenterologie und Hepatologie, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Pfeilschifter, Josef [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany); Eberhardt, Wolfgang, E-mail: w.eberhardt@em.uni-frankfurt.de [Pharmazentrum Frankfurt/ZAFES, Klinikum der Goethe-Universität Frankfurt, Frankfurt/Main (Germany)

    2015-01-01

    The impact of the RNA-binding protein HuR for the post-transcriptional deregulation of tumor-relevant genes is well established. Despite of elevations in HuR expression levels, an increase in cytoplasmic HuR abundance in many cases correlates with a high grade of malignancy. Here, we demonstrated that administration of the actin-depolymerizing macrolide latrunculin A, or blebbistatin, an inhibitor of myosin II ATPase activity, caused a dose- and time-dependent reduction in the high cytoplasmic HuR content of HepG2 and Huh7 hepatocellular carcinoma (HCC) cells. Subcellular fractionation revealed that in addition, both inhibitors strongly attenuated cytoskeletal and membrane-bound HuR abundance and conversely increased the HuR amount in nuclear cell fractions. Concomitant with changes in intracellular HuR localization, both cytoskeletal inhibitors markedly decreased the half-lives of cyclooxygenase-2 (COX-2), cyclin A and cyclin D{sub 1} encoding mRNAs resulting in a significant reduction in their expression levels in HepG2 cells. Importantly, a similar reduction in the expression of these HuR targets was achieved by a RNA interference (RNAi)-mediated knockdown of either HuR or nonmuscle myoin IIA. Using polysomal fractionation, we further demonstrate that the decrease in cytoplasmic HuR by latrunculin A or blebbistatin is accompanied by a marked change in the allocation of HuR and its mRNA cargo from polysomes to ribonucleoprotein (RNP) particles. Functionally, the basal migration and prostaglandin E{sub 2} synthesis are similarly impaired in inhibitor-treated and stable HuR-knockdown HepG2 cells. Our data demonstrate that interfering with the actomyosin-dependent HuR trafficking may comprise a valid therapeutic option for antagonizing pathologic posttranscriptional gene expression by HuR and furthermore emphasize the potential benefit of HuR inhibitory strategies for treatment of HCC. - Highlights: • We tested the effects of latrunculin A and blebbistatin on

  11. High-level Expression and Purification of Human Zona Pellucida huZP3a22-176 and huZP3b177-348 Peptides in Escherichia coli

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Objective To try making huZP3a22-176 and huZP3b177-348 polypeptides (representing an intact huZP322-348 protein without its N-terminal signal peptide and C-terminal transmembrane domain ) express in E. coli at a higher levelMethods The cDNAs encoding huZP3a and huZP3b were obtained with PCR method.The pB V221 plasmid was used to construct thermo-inducible recombinant expression vector. Purification of two target expression products employed an improved method of preparative gel polyacrylamide gel electrophoresis.Results Two polypeptides of recombinant huZP3a (rhuZP3a) and recombinant huZP3b(rhuZP3b) were all expressed respectively in an E. coli BL21(DE3)pLysS strain at a higher level, which were recognized by two specific polyclonal antisera in Western blotting test which recognize a linear B cell epitope present in rhuZP3a or rhuZP3b respectively. Using the shake-flask method, approximately 5 mg of rhuZP3a and rhuZP3b with more than 95% relative homogeneity were harvested from 1 L culture respectively.Conclusion The availability of two rhuZP3 polypeptides will help in detecting the immunogenicities of rhuZP3a and rhuZP3b through animal experiments and confirming the function domain of non-glycosylated huZP3 to induce acrosome reaction in vitro.

  12. 湖南通道县侗族村寨风水林树种资源调查%Investigation of Tree Species Resources in Feng-shui Forests of Dong Villages in Tongdao County,Hunan Province

    Institute of Scientific and Technical Information of China (English)

    吴和意; 李屈强; 朱大兴; 肖际亮; 陆安信; 杨昌知; 杨昌岩

    2014-01-01

    指出了侗族先民们把聚居村寨周围的古树林木封为“风水林”,风水林内任何人都不许砍伐林木,不许开垦,不许放牧,这种保护风水林中古树林木的习俗一直流传至今。对保存较完整的几片风水林中的树种资源进行了调查,发现风水林中的树种资源较丰富,有些属国家重点保护的珍稀濒危树种,对于保护和研究珍稀树种具有较大价值。%The ancestors of Dong people regarded the ancent trees around their villages as Feng -shui forests ,and nobody is allowed to cut down trees ,reclaim and graze in the Feng-shui forests .Such custom of protecting ancient trees in the Feng -shui forests has been maintained in the Dong communities till now .The article investigates tree species in some Feng -shui forests which have been well-protected and finds that there are abundant tree species in the Feng -shui forests ,in which some are rare and endangered species in the national protected species list .The rare tree species are valuable for conservation and further studies .

  13. Isolation, purification, crystallization, and preliminary X-ray diffraction study of the crystals of HU protein from M. gallisepticum

    Science.gov (United States)

    Nikolaeva, A. Yu.; Timofeev, V. I.; Boiko, K. M.; Korzhenevskii, D. A.; Rakitina, T. V.; Dorovatovskii, P. V.; Lipkin, A. V.

    2015-11-01

    HU proteins are involved in bacterial DNA and RNA repair. Since these proteins are absent in cells of higher organisms, inhibitors of HU proteins can be used as effective and safe antibiotics. The crystallization conditions for the M. gallisepticum HU protein were found and optimized by the vapor-diffusion method. The X-ray diffraction data set was collected to 2.91 Å resolution from the crystals grown by the vapor-diffusion method on a synchrotron source. The crystals of the HU protein belong to sp. gr. P41212 and have the following unit-cell parameters: a = b = 97.94 Å, c = 77.92 Å, α = β = γ = 90°.

  14. A Brief Discussion on Feng Youlan's Realm and Awareness Theory%境界与觉解--冯友兰境界与觉悟理论述略

    Institute of Scientific and Technical Information of China (English)

    李洪卫

    2014-01-01

    It is generally acknowledged that Feng Youlan changes the Neo-Confucianism concept into logic concept, which ap-pears to be extrinsic, though in a much better form.However, the ultimate goal of Feng Youlan's efforts is to have a good mas-tery of the universe, from which the realm of man is supposed to be greatly promoted.It is of vital importance to identify the“universal law” before the mastery of the universe.Moreover, they are complementary to each other to form an integral part, aiming to refrain from the attachment of perceptual existence through an abstract understanding of“universalia”, so as to make a self confirmation in the society and the universe.On the course of this confirmation, there embodies the participation of moral intuition in metaphysics.Feng Youlan believes that the consciousness of man attributes to the spiritual perception development. However, the proposition of spiritual perception is by its nature featured in a strong mind understanding.In his pursuit and con-firmation of “the universing self”, Feng Youlan has revised the previous ideas in a conscious or unconscious manner, which tru-ly accepts the moral self-development.Fundamentally, his proposition is neither Cheng-Zhu-styled nor Lu-Wang-styled, but a combination of Confucianism, Buddhism and Taoism with the help of western classics.According to him, in the contradiction between conceptualization and disconceptualization, not only the limitation, but also the advantage of being a route for personal moral cultivation of idea understanding can be perceived.%一般认为,冯友兰将理学概念转变为逻辑概念,超越但不内在了。但是冯友兰的目标在于对“宇宙”“大全”的把握,在此基础上使人的境界有一个根本的提升。但是对宇宙、大全把握之前必须对其有一个“性理”的确认,这两者互为表里。其目的在于通过“共相”抽象、超象的认识,超越个体对感性存在的执著,使人

  15. 论封衡养生思想对后世的影响%The Impact ofFENG Heng Health Thought on Future Generations

    Institute of Scientific and Technical Information of China (English)

    贾双保; 许俊明

    2011-01-01

    Through collecting “NingYuan XianZhi”“WuShan ShiHua”“LongXi XianZhi”these literature, from the diet,diet,exercise,patterns of life, environmental factors to explore the health culture offamous doctors FENG Hengof Donghan Dynasty,then to analyze the impact of cultural development by the health culture concept for future generations health.%通过搜集整理等文献资料,从药膳、饮食、运动、生活规律、环境因素等方面探讨东汉名医封衡的养生文化,分析其养生文化理念对后世养生文化发展的影响.

  16. Back to the Sources: A Rational Interpretation of "Feng Shui"%正本清源:理性地解读“风水”

    Institute of Scientific and Technical Information of China (English)

    王振复

    2011-01-01

    The key of ancient Chinese art of feng shui (风水) is not "to acquire water" or "to store wind", but "to assemble qi". The idea of qi(气) is the root of the theory as well as the source of feng shui studies and its art. The Book of Change puts up an ideal mo%古代中华风水术的关键并非“得水”、“藏风”,而是“聚气”,即通过“得水为上,藏风次之”的方式,使气“聚之使不散,行之使有止”。气的理念,是风水学、风水术的立论之本与人文之原。《周易》八卦方位尤其后天八卦方位,奠定了风水的理想模式。作为古代中华的一种巫性文化,风水具有“畏天”兼“知命”的二重性,既背反又合一。在中华古代风水学(木)中,人是一个有趣而尴尬的角色,糊涂与清醒同在,迷信同理智并存,委琐和尊严兼有,崇拜携审美偕行。迷信风水命理,只能导致人类居住的困难。理性,尤其科学理性,作为人性中的高贵部分,应当予

  17. HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells

    Directory of Open Access Journals (Sweden)

    Ennifar Eric

    2008-06-01

    Full Text Available Abstract Reverse transcription of the genetic material of human immunodeficiency virus type 1 (HIV-1 is a critical step in the replication cycle of this virus. This process, catalyzed by reverse transcriptase (RT, is well characterized at the biochemical level. However, in infected cells, reverse transcription occurs in a multiprotein complex – the reverse transcription complex (RTC – consisting of viral genomic RNA associated with viral proteins (including RT and, presumably, as yet uncharacterized cellular proteins. Very little is known about the cellular proteins interacting with the RTC, and with reverse transcriptase in particular. We report here that HIV-1 reverse transcription is affected by the levels of a nucleocytoplasmic shuttling protein – the RNA-binding protein HuR. A direct protein-protein interaction between RT and HuR was observed in a yeast two-hybrid screen and confirmed in vitro by homogenous time-resolved fluorescence (HTRF. We mapped the domain interacting with HuR to the RNAse H domain of RT, and the binding domain for RT to the C-terminus of HuR, partially overlapping the third RRM RNA-binding domain of HuR. HuR silencing with specific siRNAs greatly impaired early and late steps of reverse transcription, significantly inhibiting HIV-1 infection. Moreover, by mutagenesis and immunoprecipitation studies, we could not detect the binding of HuR to the viral RNA. These results suggest that HuR may be involved in and may modulate the reverse transcription reaction of HIV-1, by an as yet unknown mechanism involving a protein-protein interaction with HIV-1 RT.

  18. Domain-specific phosphomimetic mutation allows dissection of different protein kinase C (PKC) isotype-triggered activities of the RNA binding protein HuR.

    Science.gov (United States)

    Schulz, Sebastian; Doller, Anke; Pendini, Nicole R; Wilce, Jacqueline A; Pfeilschifter, Josef; Eberhardt, Wolfgang

    2013-12-01

    The ubiquitous mRNA binding protein human antigen R (HuR) participates in the post-transcriptional regulation of many AU-rich element (ARE)-bearing mRNAs. Previously, by using in vitro kinase assay, we have identified serines (Ser) 158, 221 and 318 as targets of protein kinase C (PKC)-triggered phosphorylation. In this study, we tested whether GFP- or GST-tagged HuR constructs bearing a phosphomimetic Ser (S)-to-Asp (D) substitution at the different PKC target sites, would affect different HuR functions including HuR nucleo-cytoplasmic redistribution and binding to different types of ARE-containing mRNAs. The phosphomimetic GFP-tagged HuR protein bearing a phosphomimetic substitution in the hinge region of HuR (HuR-S221D) showed an increased cytoplasmic abundance when compared to wild-type HuR. Conversely, data from in vitro kinase assay and electrophoretic mobility shift assay (EMSA), implicates that phosphorylation at Ser 221 is not relevant for mRNA binding of HuR. Quantification of in vitro binding affinities of GST-tagged wild-type HuR and corresponding HuR proteins bearing a phosphomimetic substitution in either RRM2 (HuR-S158D) or in RRM3 (HuR-S318D) by microscale thermophoresis (MST) indicates a specific binding of wild-type HuR to type I, II or type III-ARE-oligonucleotides in the high nanomolar range. Interestingly, phosphomimetic mutation at position 158 or 318 had a negative influence on HuR binding to type I- and type II-ARE-mRNAs whereas it significantly enhanced HuR affinity to a type III-ARE substrate. Our data suggest that differential phosphorylation of HuR by PKCs at different HuR domains coordinates subcellular HuR distribution and leads to a preferential binding to U-rich bearing target mRNA.

  19. A Review of the Pharmacological Effects of the Dried Root of Polygonum cuspidatum (Hu Zhang and Its Constituents

    Directory of Open Access Journals (Sweden)

    Huan Zhang

    2013-01-01

    Full Text Available Traditional Chinese medicine (TCM has been widely used in China for thousands of years to treat and prevent diseases. TCM has been proven safe and effective, and it is being considered as one of the important types of complementary and alternative medicine and receives increasing attention worldwide. The dried root of Polygonum cuspidatum Sieb. et Zucc. (also known as “Hu Zhang” in Chinese is one of the medicinal herbs listed in the Pharmacopoeia of the People's Republic of China. Hu Zhang is widely distributed in the world. It can be found in Asia and North America and is used as folk medicine in countries such as Japan and Korea. In China, Hu Zhang is usually used in combination with other TCM herbs. The therapeutic uses of those Hu Zhang-containing TCM prescriptions or formulations are for treating cough, hepatitis, jaundice, amenorrhea, leucorrhea, arthralgia, burns and snake bites. Recent pharmacological and clinical studies have indicated that Hu Zhang has antiviral, antimicrobial, anti-inflammatory, neuroprotective, and cardioprotective functions. This review gives a summary of the reported therapeutic effects of the active compounds and the different extracts of Hu Zhang.

  20. A Study on the FengShui Ideas and Application in Three Kingdom Period in Korea%朝鲜半岛三国时代对中国风水的认识与接纳

    Institute of Scientific and Technical Information of China (English)

    金惠贞[韩国

    2012-01-01

    The inducing time of traditional Chinese FengShui to Korea, has been crossed lips generally with the inducing time of Buddhism. That is, it is generally considered to be the beginning period of 8C. of SinLuo Dynasty. But it should be regarded as Three Kingdom Period in Korea. Long before Three Kingdom Period, in GaoJuLi region, the dragon which was closely connected with Feng- Shui ideas thousands years ago had been treated as an important creature, and ancient stones were constructed on the basis of geograph- ical features, solemn funeral, and distinct bearing thoughts. This means that before the inducement of Chinese FengShui through the cultural correspondence with China, it had its own geographical ideas, and that there was basic soil to induce Chinese FengShui or something Bimilar with Fen~Shui. In this envirionment, LuoPan and the defense tombs system of GaoJuLi, Zhen's epitaph, GaoJuLi tombs wall painting and an astronomical chart, the positioning of tombs of JiaYe Dynasty and remains, the positioning of BaiJi WuNing king~ tomb, its structure and remains, the recording of old books of Korea, shows that Chinese FengShui was well known to people and actually vigorously adopted on the structure of tombs in Three Kingdom Period. This provides the possibility that Chinese FengShui made contributions to some extent on the choice of location of the temples which had been constructed in Three Kingdom Period, and have continued to prosper until today.%中国风水不是在统一新罗时期传来的,在这之前的三国时代对中国风水的认识与接纳是很明显的,风水成为三国时代的主要文化因素,并在墓葬中应用了中国风水。《崇福寺碑文》的风水内容及佛国寺的建立,是经过几百年的风水认识与实践以后,对中国风水接纳的结果。

  1. CONSTRUCTION OF HU-PBL/SCID CHIMERAS AND DEVELOPMENT OF EBV-RELATED LYMPHOMAS

    Institute of Scientific and Technical Information of China (English)

    Run-liang Gan; Ke Lan; Zhi-hua Yin; Li-jiang Wang; Ying Song; Kai-tai Yao

    2005-01-01

    Objective To construct hu-PBL/SCID chimeras and to investigate the development of lymphoma and oncogenicity of the Epstein-Barr virus (EBV).Mtehods Human peripheral blood lymphocytes (PBLs) were isolated from healthy adult donors and transplanted intraperitoneally into severe combined immunodeficient (SCID) mice. Mice with hu-PBL engraftment from healthy EBV seronegative donors were injected intraperitoneally with EBV-containing supematant from suspension culture of B95-8 cell line (active infection), whereas mice receiving lymphocytes from healthy EBV seropositive donors were not re-infected with B95-8 derived EBV (latent infection). Pathological examination and molecular analysis were performed on experimental animals and induced neoplasms.Results In the early stage of this experiment, 12 mice died of acute graft-versus-host disease, mortality was 34.3%(12/35 mice) with an average life span of 17.5 days. In 19 survival hu-PBL/SCID chimeric recipients from 12 healthy donors,tumor incidence was 84.2% (16/19 mice). The average survival time of tumor-bearing mice was 65.5 days. EBV-related neoplasms in SCID mice were nodular tumors with aggressive and fatal features. Histological morphology of tumors exhibited diffuse large cell lymphomas. Immunohistochemistry revealed that LCA (CD45) and L26 (CD20) were positive, but both PS1 (CD3) and UCHL-1 (CD45RO) were negative, and EBV products ZEBRA, LMP1, and EBNA2 were expressed in a small number of tumor cells. EB virus particles were seen in the nuclei of some tumor cells by electron microscopy, and EBV DNA could be amplified in the tumor tissues by PCR. In situ hybridization indicated that the nuclei of tumor cells contained human-specific Alu sequence.Conclusions EBV-induced tumors were human B-cell malignant lymphomas. We obtained direct causative evidence dealing with EBV-associated tumor deriving from normal human cells.

  2. 'Hu Hong' (bad thing): parental perceptions of teenagers' sexuality in urban Vietnam.

    Science.gov (United States)

    Do, Lan Anh Thi; Boonmongkon, Pimpawun; Paek, Seung Chun; Guadamuz, Thomas E

    2017-02-28

    Teenagers under 18 years old in Vietnam are considered as minors who usually lack the autonomy to make decisions. They are also sometimes viewed as contributors to social evils including crime, violence and substance use. Moreover, most Vietnamese teenagers have unsafe sex before marriage. The objective of this study is to explore the parental perceptions relating to their teenagers' sexuality, particularly the social and cultural forces, that may hinder access to sexuality information. Guided by a Community Advisory Board (CAB), this qualitative study uses four focus group discussions (FGDs) consisting of 12 mothers and 12 fathers, as well as twelve individual in-depth interviews (IDIs) with a diverse sample of parents of teens in Ho Chi Minh City (HCMC), Vietnam. Content and discourse analysis were conducted, based on Foucauldian concepts. Four themes emerged: 1) Meanings of sexuality and sexuality education, 2) Early sexual intercourse destroys teenagers' future, 3) Teenagers are not hu hong (spoil/bad thing), are innocent and virgin, and 4) Policing and controlling of sexual intercourse among teens. Parents did not view their teenage children as sexual beings; those who are sexual are considered hu hong. Parents believed that teens need to be policed and controlled to prevent them from becoming hu hong, particularly girls. Controlling of sexuality information by parents was therefore common in HCMC, but differed by gender and educational levels of parents. For example, fathers more than mothers were not comfortable teaching their teenage children about sex and sexuality. Parents with higher education police their teenage children's usage of the Internet and social media, while parents with lower education control who can be friends with their teenage children. Vietnamese parents in general have negative views of sex and sexuality education for their teenage children. Recognizing that many Vietnamese teenagers have unsafe sex before marriage, parents need to

  3. Effects of weaning age on growth, nutrient digestibility and metabolism, and serum parameters in Hu lambs

    Directory of Open Access Journals (Sweden)

    Jianmin Chai

    2015-12-01

    Full Text Available This study was conducted to investigate the effect of weaning age on growth performance, nutrient digestion and metabolism, and serological indicators, and to obtain an optimal weaning age in Hu lambs. Forty-eight newborn Hu lambs (birth weight, 2.53 ± 0.14 kg were randomly divided into 4 groups. The lambs in control group (ER suckled their dams. The lambs in other three experimental groups were weaned on milk replacer at 10, 20, and 30 days of age (EW10, EW20, and EW30 groups, respectively. The results were as follows: 1 lambs in EW10 and EW30 groups had a lower (P  0.05 among groups; however, the apparent digestibility and deposition of calcium in early weaned lambs were lower (P < 0.05 than those in ewe-reared lambs. 4 The albumin content in EW30 group was lower (P < 0.05 than that in ER group; the globulin content in EW30 group was higher (P < 0.05 than that in other groups; the content of serum insulin-like growth factor-Ⅰ in weaned lambs tended to increase compared with lambs in ER group. Finally, the growth rate of lambs decreased within 10 days post-weaning, but early weaning boosted creep feed intake, leading to better growth and health later in life. The Hu lambs can be weaned on milk replacer and creep feed at 10 days of age.

  4. Chemical Constituents from the Seeds of Ziziphus jujuba var. Spinosa (Bunge) Hu

    Institute of Scientific and Technical Information of China (English)

    Li-Mei LI; Xun LIAO; Shu-Lin PENG; Li-Sheng DING

    2005-01-01

    To search for new and bioactive minor components from traditional Chinese medicines, a new compound, named jujuphenoside (1), was isolated from the seeds of Ziziphusjujuba var. spinosa (Bunge)Hu. The structure of jujuphenoside was elucidated by spectral and chemical methods, particularly twodimensional nuclear magnetic resonance analysis. Together with the new compound, 22 known compounds were also isolated and identified from the seeds of Z. jujuba var. spinosa, among which, epiceanothic acid (2) was first obtained from natural resources, whereas compounds 7-16 were first obtained from this plant.

  5. Opsoclonus-myoclonus and anti-Hu positive limbic encephalitis in a patient with neuroblastoma.

    Science.gov (United States)

    Morales La Madrid, Andres; Rubin, Charles M; Kohrman, Michael; Pytel, Peter; Cohn, Susan L

    2012-03-01

    Opsoclonus-myoclonus syndrome (OMS) is seen in 2-3% of children with neuroblastoma and is believed to be caused by an autoimmune process elicited by the tumor. Although long-term neurologic sequelae are common in children with OMS, limbic encephalitis has not previously been reported. We report a child who developed limbic encephalitis associated with anti-Hu antibodies, 6 years after her initial diagnosis of neuroblastoma and OMS. This case demonstrates that patients with neuroblastoma and OMS are at risk for developing new paraneoplastic symptoms years after their original diagnosis and emphasizes the need for careful long-term follow-up.

  6. Micología Lista de Huéspedes Colombianos

    Directory of Open Access Journals (Sweden)

    Obregon Botero Rafael

    1964-12-01

    Full Text Available El presente trabajo es una lista de hongos encontrados en Colombia. Está ordenada por Familias del huésped y cada nombre científico del hongo va seguido por la mención de los lugares donde fue encontrado así como la altura de tales localidades. Luego precede un número que corresponde a la colección existente en el Ministerio de Agricultura de Colombia. No pretendo sino iniciar la labor que han de completar especialistas mejor informados y espero que sea de alguna utilidad para los que se dedican a esta rama de las Ciencias Naturales.

  7. Hybrid Semiloof elements for plates and shells based upon a modified Hu-Washizu principle

    Science.gov (United States)

    Pian, T. H. H.; Sumihara, K.

    1984-01-01

    Hybrid SemiLoof elements for plates and shells are developed based upon modified Hu-Washizu principle. In the new version of the assumed stress hybrid formulation the equilibrium equations are satisfied through the introduction of internal displacement parameters as Lagrange multipliers. The inversion of the resulting H-matrices is simplified particularly when the stresses are expressed in terms of natural coordinates. A 24-DOF triangular element and a 32-DOF quadrilateral element based on shallow shell theory are derived and evaluated.

  8. HuLiS, a program to teach mesomerism and more

    Science.gov (United States)

    Carissan, Yannick; Goudard, Nicolas; Hagebaum-Reignier, Denis; Humbel, Stéphane

    2016-08-01

    The HuLiS program is presented as a tool to decompose a delocalized wave function as a linear combination of localized electronic structures. The principles of the energy based HL-CI and the overlap based schemes HL-P are developed. The results obtained for a set of 10 relevant organic molecules are shown to compare very well with high level quantum chemistry calculations. A trust factort is introduced and its use is shown on the allyl radical case where symmetry must be taken into account.

  9. MicroRNA-16 Modulates HuR Regulation of Cyclin E1 in Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Xun Guo

    2015-03-01

    Full Text Available RNA binding protein (RBPs and microRNAs (miRNAs or miRs are post-transcriptional regulators of gene expression that are implicated in development of cancers. Although their individual roles have been studied, the crosstalk between RBPs and miRNAs is under intense investigation. Here, we show that in breast cancer cells, cyclin E1 upregulation by the RBP HuR is through specific binding to regions in the cyclin E1 mRNA 3' untranslated region (3'UTR containing U-rich elements. Similarly, miR-16 represses cyclin E1, dependent on its cognate binding sites in the cyclin E1 3'UTR. Evidence in the literature indicates that HuR can regulate miRNA expression and recruit or dissociate RNA-induced silencing complexes (RISC. Despite this, miR-16 and HuR do not affect the other’s expression level or binding to the cyclin E1 3'UTR. While HuR overexpression partially blocks miR-16 repression of a reporter mRNA containing the cyclin E1 3'UTR, it does not block miR-16 repression of endogenous cyclin E1 mRNA. In contrast, miR-16 blocks HuR-mediated upregulation of cyclin E1. Overall our results suggest that miR-16 can override HuR upregulation of cyclin E1 without affecting HuR expression or association with the cyclin E1 mRNA.

  10. 可在鱼体内表达hu-IFN-α的基因重组构建及转化%Construction of a Fish Expression hu-IFN-α Recombinant Gene

    Institute of Scientific and Technical Information of China (English)

    张学文; 章怀云; 符少辉; 陈韬; 陈立祥; 肖调义

    2001-01-01

    通过分子重组,将人α-干扰素(hu-IFN-α)基因编码序列克隆到鲤鱼β-肌动蛋白基因启动子下游,构建成能在鱼体内组成型表达hu-IFN-α的基因重组分子。经限制性酶切分析、Southern杂交和序列测定,证明重组分子构建的正确性。采用显微注射法将这一重组基因转化草鱼受精卵,获得了表达人α-干扰素的转基因草鱼。%The coding sequence of hu-IFN-α gene is cloned under the control of Carp β-actin gene promoter. Thus a fish constantly expression of hu-IFN-α gene recombinant is constructed. Southern blot and sequencing results show that the recombinant molecule is correctly constructed. The recombinant gene is transferred into fertilized zygotes of grass carp via microinjection. And hu-IFN-α is detected out in 20% of transgenic fish by ELISA.

  11. HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes

    Directory of Open Access Journals (Sweden)

    Apostolia Guialis

    2013-10-01

    Full Text Available Post-transcriptional regulatory networks are dependent on the interplay of many RNA-binding proteins having a major role in mRNA processing events in mammals. We have been interested in the concerted action of the two RNA-binding proteins hnRNP A1 and HuR, both stable components of immunoselected hnRNP complexes and having a major nuclear localization. Specifically, we present here the application of the RNA-immunoprecipitation (RIP-Chip technology to identify a population of nuclear transcripts associated with hnRNP A1-RNPs as isolated from the nuclear extract of either HuR WT or HuR-depleted (KO mouse embryonic fibroblast (MEF cells. The outcome of this analysis was a list of target genes regulated via HuR for their association (either increased or reduced with the nuclear hnRNP A1-RNP complexes. Real time PCR analysis was applied to validate a selected number of nuclear mRNA transcripts, as well as to identify pre-spliced transcripts (in addition to their mature mRNA counterpart within the isolated nuclear hnRNP A1-RNPs. The differentially enriched mRNAs were found to belong to GO categories relevant to biological processes anticipated for hnRNP A1 and HuR (such as transport, transcription, translation, apoptosis and cell cycle indicating their concerted function in mRNA metabolism.

  12. Effects of rHu-EPO on Myocyte Apoptosis and Cardiac Function Follow-ing Acute Myocardial Infarction in Rats

    Institute of Scientific and Technical Information of China (English)

    YE Liang; DU Xinling; XIA Jiahong; JIANG Ping

    2005-01-01

    The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infarction in rats were studied. Thirty-two rats were divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI+ EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery. rHu-EPO was administered i. p. in MI+EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI+EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2, bax) and TUNEL dyeing. The results showed that hemodynamic function in MI+ EPO group was much better than in MI group.The number of the cells positive for bax and TUNEL in MI+EPO group was less than that in MI group. The number of the cells positive for bcl-2 in MI+EPO group was more than that in MI group. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function.

  13. The Use of Soluble Transferrin Receptor in the Detection of rHuEPO Abuse in Sports

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    Lorenzo Gordon

    2010-02-01

    Full Text Available Erythropoietin (EPO increases the number of circulating erythrocytes and muscle oxygenation. The recombinant forms of EPO have indiscriminately been used by athletes, mainly in endurance sports to increase their erythrocytes concentration, thus generating a better delivery of oxygen to the muscle tissue. The administration of recombinant human erythropoietin (rHuEPO except for therapeutic use was prohibited by the International Olympic Committee (IOC and its unauthorized use considered as doping. In the last few years, a number of studies using parameters indicative of accelerated erythropoiesis have investigated a number of indirect methods for the detection of rHuEPO abuse. No single indirect marker has been found that can satisfactorily demonstrated rHuEPO misuse. Soluble transferrin receptor (sTfR is a new marker of iron status and erythropoietic activity. It has been included in multivariable blood testing models for the detection of performance enhancing EPO abuse in sports. Indirect markers of altered erythropoiesis give reliable evidence of current or discontinued rHuEPO usage. This review describes the physical, biological and pharmacokinetic properties of endogenous EPO and its recombinant form. It also discusses the available strategies for the detection of rHuEPO abuse in sports, involving the use of sTfR concentration directly or in mathematical multivariate models.

  14. Potential Risk of Hybridization in Ex Situ Collections of Two Endangered Species of Sinojackia Hu (Styracaceae)

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Spontaneous hybridization in ex situ facilities can undermine the genetic integrity of ex situ collections and potentially contaminate open-pollinated seeds or seedlings destined for the reintroduction of endangered plant species into the wild. In the present study, the potential risk of hybridization between two endangered Chinese endemic species, namely Sinojackia xylocarpa Hu and S. rehderiana Hu, which are naturally allopatric species but were conserved ex situ in Wuhan Botanical Garden (WBG), Wuhan, China, were investigated over three consecutive years from 2003 to 2005. The entire overlapping flowering period of the two species was 14-20 d and the two species shared the same pollinator insects during the entire flowering season in WBG. The floral isolation between the two species was not an issue in the ex situ collection at WBG. The results suggest an opportunity for pollen transfer between species and a potential risk of genetic introgression and loss of genetic identity of open-pollinated seeds produced in the ex situ collection of these two endangered species. An artificial reciprocal cross between S. xylocarpa and S. rehderiana confirmed that the two congener species could readily set seeds, indicating no post-pollination barriers to hybridization and the importance of spatial isolation as a barrier to inter-specific crossing. Therefore, to manage these crossable species with overlapping flowering times and shared pollination vectors in ex situ facilities, spatial isolation should be carefully considered to minimize the possibility of spontaneous hybridization.

  15. Combining wavelets transform and Hu moments with self-organizing maps for medical image categorization

    Science.gov (United States)

    Silva, Leandro A.; Del-Moral-Hernandez, Emilio; Moreno, Ramon A.; Furuie, Sérgio S.

    2011-10-01

    Images are fundamental sources of information in modern medicine. The images stored in a database and divided in categories are an important step for image retrieval. For an automatic categorization process, detailed analysis is done regarding image representation and generalization method. The baseline method for this process, in the medical image context, is using thumbnails and K-nearest neighbor (KNN), which is easily implemented and has had satisfactory results in literature. This work addresses an alternative method for automatic categorization, which jointly uses discrete wavelet transform with Hu's moments for image representation and self-organizing maps (SOM) neural networks combined with the KNN classifier (SOM-KNN), for medical image categorization. Furthermore, extensive experiments are conducted, to define the best wavelet family and to select the best coefficients set, to consider the remaining wavelet coefficients set (not selected as the best ones) through their Hu's moments, and to carry out a contrastive study with other successful approaches for categorization. The categorization result from a database with 10,000 images in 116 categories yielded 81.8% of correct rate, which is much better than the 67.9% obtained by the baseline method; and the time consumed in classification processing with SOM-KNN is 100 times shorter than KNN.

  16. Hu1-2: a metal-poor bipolar planetary nebula with fast collimated outflows

    CERN Document Server

    Fang, Xuan; Miranda, Luis F; Riera, Angels; Velazquez, Pablo F; Raga, Alejandro C

    2015-01-01

    We present narrow-band optical and near-IR imaging and optical long-slit spectroscopic observations of Hu1-2, a Galactic planetary nebula (PN) with a pair of [N II]-bright, fast-moving (> 340 km/s) bipolar knots. Intermediate-dispersion spectra are used to derive physical conditions and abundances across the nebula, and high-dispersion spectra to study the spatio-kinematical structure. Generally Hu1-2 has high He/H (~0.14) and N/O ratios (~0.9), typical of Type I PNe. On the other hand, its abundances of O, Ne, S, and Ar are low as compared with the average abundances of Galactic bulge and disc PNe. The position-velocity maps can be generally described as an hour-glass shaped nebula with bipolar expansion, although the morphology and kinematics of the innermost regions cannot be satisfactorily explained with a simple, tilted equatorial torus. The spatio-kinematical study confines the inclination angle of its major axis to be within 10 degrees of the plane of sky. As in the irradiated bow-shocks of IC4634 and ...

  17. Revisiting the proposed planetary system orbiting the eclipsing polar HU Aquarii

    CERN Document Server

    Wittenmyer, Robert A; Marshall, J P; Butters, O W; Tinney, C G

    2011-01-01

    It has recently been proposed, on the basis of eclipse-timing data, that the eclipsing polar cataclysmic variable HU Aquarii is host to at least two giant planets. However, that result has been called into question based upon the dynamical stability of the proposed planets. In this work, we present a detailed re-analysis of all eclipse timing data available for the HU Aquarii system, making use of standard techniques used to fit orbits to radial-velocity data. We find that the eclipse timings can be used to obtain a two-planet solution that does not require the presence of additional bodies within the system. We then perform a highly detailed dynamical analysis of the proposed planetary system. We show that the improved orbital parameters we have derived correspond to planets that are dynamically unstable on unfeasibly short timescales (of order 10^4 years or less). Given these results, we discuss briefly how the observed signal might in fact be the result of the intrinsic properties of the eclipsing polar, r...

  18. ReHuNa- La Red por la Humanización del Parto y Nacimiento

    Directory of Open Access Journals (Sweden)

    Daphne RATTNER

    2010-12-01

    Full Text Available La ReHuNa - Red por la Humanización del Parto y Nacimiento - es una organización de la sociedad civil que actúa desde 1993 en forma de red de asociados en el Brasil. Su principal objetivo es la divulgación de la asistencia y cuidados perinatales con base en evidencias científicas y de acuerdo a las recomendaciones de la Organización Mundial de la Salud. Esta red tuvo un papel fundamental en la estructura de un movimiento que hoy se denomina “humanización de la asistencia al parto y nacimiento”, que pretende disminuir las intervenciones innecesarias y promover cuidados al proceso del embarazo-parto-nacimiento-lactancia comprendido como un proceso natural y fisiológico basado en conocimientos científicos. Este artículo relata la historia de la ReHuNa y sus principales realizaciones y señala sus caminos futuros.

  19. The impact of the vaccination program for hemorrhagic fever with renal syndrome in Hu County, China.

    Science.gov (United States)

    Xiao, Dan; Wu, Kejian; Tan, Xin; Yan, Tiecheng; Li, Haitao; Yan, Yongping

    2014-02-03

    The incidence of hemorrhagic fever with renal syndrome (HFRS) in Hu County ranked third of all counties in China in 2010. Although this county has provided a HFRS vaccination program freely since 1994, the impact of HFRS remains quite substantial. In order to continue the vaccination program effectively and control HFRS, a detailed understanding of the effect of the vaccination program should be undertaken. The Cochran-Armitage trend test was employed to examine the temporal trends of HFRS incidences, mortality rate and vaccination compliance. Temporal cluster analysis was performed to detect time periods of high HFRS risk. Cross correlation analysis was conducted to detect the correlation between HFRS incidence and vaccination compliance. Wavelet analysis was employed to detect the shift of the periodicity of HFRS. Between 1971 and 2011, the HFRS incidence and mortality rate ranged from 9.53/100,000 to 300.57/100,000 and 0 to 24.91/100,000, respectively, with a fluctuating but distinctly declining trend (incidence: Z=-34.38, P1994. In conclusion, the increase in vaccination compliance may play an important role in HFRS control and prevention in Hu County, China.

  20. The SCID-hu mouse as a model for HIV-1 infection.

    Science.gov (United States)

    Aldrovandi, G M; Feuer, G; Gao, L; Jamieson, B; Kristeva, M; Chen, I S; Zack, J A

    1993-06-24

    During normal fetal ontogeny, one of the first organs to harbour CD4-positive cells is the thymus. This organ could therefore be one of the earliest targets infected by human immunodeficiency virus type 1 (HIV-1) in utero. HIV-1-infected cells and pathological abnormalities of the thymus have been seen in HIV-1-infected adults and children, and in some fetuses aborted from infected women. Studies of HIV-1 pathogenesis have been hampered by lack of a suitable animal model system. Here we use the SCID-hu mouse as a model to investigate the effect of virus infection on human tissue. The mouse is homozygous for the severe combined immunodeficiency (SCID) defect. The model is constructed by implanting human fetal liver and thymus under the mouse kidney capsule. A conjoint human organ develops, which allows normal maturation of human thymocytes. After direct inoculation of HIV-1 into these implants, we observed severe depletion of human CD4-bearing cells within a few weeks of infection. This correlated with increasing virus load in the implants. Thus the SCID-hu mouse may be a useful in vivo system for the study of HIV-1-induced pathology.

  1. RNA-binding protein HuD reduces triglyceride production in pancreatic β cells by enhancing the expression of insulin-induced gene 1.

    Science.gov (United States)

    Kim, Chongtae; Lee, Heejin; Kang, Hoin; Shin, Jung Jae; Tak, Hyosun; Kim, Wook; Gorospe, Myriam; Lee, Eun Kyung

    2016-04-01

    Although triglyceride (TG) accumulation in the pancreas leads to β-cell dysfunction and raises the chance to develop metabolic disorders such as type 2 diabetes (T2DM), the molecular mechanisms whereby intracellular TG levels are regulated in pancreatic β cells have not been fully elucidated. Here, we present evidence that the RNA-binding protein HuD regulates TG production in pancreatic β cells. Mouse insulinoma βTC6 cells stably expressing a small hairpin RNA targeting HuD (shHuD) (βTC6-shHuD) contained higher TG levels compared to control cells. Moreover, downregulation of HuD resulted in a decrease in insulin-induced gene 1 (INSIG1) levels but not in the levels of sterol regulatory element-binding protein 1c (SREBP1c), a key transcription factor for lipid production. We identified Insig1 mRNA as a direct target of HuD by using ribonucleoprotein immunoprecipitation (RIP) and biotin pulldown analyses. By associating with the 3'-untranslated region (3'UTR) of Insig1 mRNA, HuD promoted INSIG1 translation; accordingly, HuD downregulation reduced while ectopic HuD expression increased INSIG1 levels. We further observed that HuD downregulation facilitated the nuclear localization of SREBP1c, thereby increasing the transcriptional activity of SREBP1c and the expression of target genes involved in lipogenesis; likewise, we observed lower INSIG1 levels in the pancreatic islets of HuD-null mice. Taken together, our results indicate that HuD functions as a novel repressor of lipid synthesis in pancreatic β cells.

  2. Clinical Experience of Syndrome Differentiation and Treatment of Psoriasis by Feng Xianzhang%冯宪章辨证治疗银屑病的临床经验

    Institute of Scientific and Technical Information of China (English)

    郑志广

    2015-01-01

    Psoriasis can be divided into 7 syndromes by Feng Xianzhang:wind heat and blood heat,blood stasis and blood dryness,blood deficiency and wind dryness,wind-dampness violate exterior,imbalance of Chong and Ren,damp-heat stagnancy,blood deficiency and hyperactivity of the liver.Respectively treating by dispelling wind and clearing heat,cooling and invigorate blood,promoting blood circu-lation to remove blood stasis,dredging collaterals and eliminating stagnation,nourishing blood and skin moisten,dispelling wind and ar-resting itching,dispersing wind and removing dampness,invigoration of Chong Ren,clearing heat and promoting diuresis,nourishing blood and liver.Feng Xianzhang make a diagnosis and give treatment of psoriasis focus on overall adjustment,not treatment of skin le-sion.Starting from the overall concept,in order to cure the disease by adjusting the function of viscera,treating the inner and the more its outside.At the same time pay attention to regulating liver,harmonizing Qi and blood,pay attention to science education and psycho-logical counseling.%冯宪章将银屑病分为风热血热证、血瘀血燥证、血虚风燥证、风湿袭表证、冲任失调证、湿热郁遏证、血虚肝旺证。分别采用疏风清热、凉血活血法,活血化瘀、通络散结法,养血润肤、祛风止痒法,散风除湿法,调补冲任法,清热利湿法,养血柔肝法以治之。冯宪章诊治银屑病注重整体调节,不提倡针对皮损的治疗。从整体观念出发,以调整人体脏腑功能失调,治其内而愈其外,达到“治其人”而“愈其病”的目的。临证注重调肝与调和气血,同时注重科普教育与心理疏导。

  3. Issues in the Studies on Scientific Thought of Xue Feng-zuo%薛凤祚科学思想研究的若干问题

    Institute of Scientific and Technical Information of China (English)

    马来平

    2012-01-01

    关于《历学会通》对哥白尼日心体系的修改问题,科学史工作者提供的证据颇有说服力,应予以认真对待,但尚有探讨余地;明末清初正是中国传统天文历法、数学与占验开始萌发分离动向的时期,薛凤祚是这一潮流的见证人和主要参与者。其占验思想是其科学思想的有机组成部分,应给予宽容而客观的分析和评价;薛凤祚的科学观是其科学思想的核心,研究它须以近代科学观为参照系,如此,方能正确判断其科学观的性质,厘清其中弥足珍贵的近代因素。%Concerning the amendation of Copernicus's Heliocentric theory in Li Xue Hui Tong,historians of science have provided convincing evidences,which should be taken seriously and needs further investigation.Xue Feng-zuo was the witness and the main participant in the trend of Chinese traditional astronomy,calendar and mathematics dissociating from divination during the period of late Ming Dynasty and early Qing Dynasty.His thought on divination is an integral part of his scientific thought system,which should be given to tolerant and objective analysis and evaluation.Xue Feng-zuo's view of science is the core of his scientific thoughts,on which researches need to be based on the modern view of science to determine the nature of his view of science and clarify the precious modern factors from it.

  4. HuR-targeted nanotherapy in combination with AMD3100 suppresses CXCR4 expression, cell growth, migration, and invasion in lung cancer

    Science.gov (United States)

    Muralidharan, Ranganayaki; Panneerselvam, Janani; Chen, Allshine; Zhao, Yan Daniel; Munshi, Anupama; Ramesh, Rajagopal

    2015-01-01

    The CXCR4 chemokine receptor plays an important role in cancer cell metastasis. The CXCR4 antagonist, AMD3100, has limited efficacy in controlling metastasis. HuR, an RNA-binding protein, regulates CXCR4 in cancer cells. We therefore investigated whether targeting HuR using a siRNA-based nanoparticle plus AMD3100 would suppress CXCR4 and inhibit lung cancer metastasis. We treated human H1299 lung cancer cell with HuR-specific siRNA contained in a folate-targeted lipid nanoparticle (HuR-FNP) plus AMD3100, and compared this with AMD3100 alone, HuR-FNP alone and no treatment. HuR-FNP plus AMD3100 treatment produced a G1 phase cell-cycle arrest and reduced cell viability above and beyond the effects of AMD3100 alone. HuR and CXCR4 mRNA and protein expression levels were markedly reduced in all treatment groups. Phosphorylated (p) AKTS473 protein was also reduced. P27 protein expression increased with HuR-FNP and combination treatment. Promoter-based reporter studies showed that the combination inhibited CXCR4 promoter activity more than did either treatment alone. Cell migration and invasion was significantly reduced with all treatment; the combination provided the most inhibition. Reduced matrix metalloprotease (MMP) -2 and -9 expression was associated with reduced invasion in all treatment groups. Thus, we found that combined HuR and CXCR4 targeting effectively controlled lung cancer metastasis. PMID:26494555

  5. HuR-targeted nanotherapy in combination with AMD3100 suppresses CXCR4 expression, cell growth, migration and invasion in lung cancer.

    Science.gov (United States)

    Muralidharan, R; Panneerselvam, J; Chen, A; Zhao, Y D; Munshi, A; Ramesh, R

    2015-12-01

    The CXCR4 chemokine receptor has an important role in cancer cell metastasis. The CXCR4 antagonist, AMD3100, has limited efficacy in controlling metastasis. HuR, an RNA-binding protein, regulates CXCR4 in cancer cells. We therefore investigated whether targeting HuR using a siRNA-based nanoparticle plus AMD3100 would suppress CXCR4 and inhibit lung cancer metastasis. We treated human H1299 lung cancer cells with HuR-specific siRNA contained in a folate-targeted lipid nanoparticle (HuR-FNP) plus AMD3100, and compared this with AMD3100 alone, HuR-FNP alone and no treatment. HuR-FNP plus AMD3100 treatment produced a G1 phase cell cycle arrest and reduced cell viability above and beyond the effects of AMD3100 alone. HuR and CXCR4 mRNA and protein expression levels were markedly reduced in all treatment groups. Phosphorylated (p) AKT(S473) protein was also reduced. P27 protein expression increased with HuR-FNP and combination treatment. Promoter-based reporter studies showed that the combination inhibited CXCR4 promoter activity more than did either treatment alone. Cell migration and invasion was significantly reduced with all treatments; the combination provided the most inhibition. Reduced matrix metalloprotease (MMP)-2 and -9 expression was associated with reduced invasion in all treatment groups. Thus, we found that combined HuR and CXCR4 targeting effectively controlled lung cancer metastasis.

  6. 自拟痛风汤加减治疗老年原发性痛风30例%Modified Self-made TongFengTang in Treating 30 Cases of Senile Primary Gout

    Institute of Scientific and Technical Information of China (English)

    孙涛

    2012-01-01

    目的:观察自拟痛风汤加减治疗老年原发性痛风的疗效.方法:以自拟痛风汤为基础方辨证加减,治疗痛风30例,1荆/d,7天1个疗程,治疗1~3个疗程后观察临床疗效及血尿酸变化情况.结果:总有效率为93.3%.结论:自拟痛风汤治疗痛风疗效显著.%Objective: To observe therapeutic effects of modified self-made TongFengTang in treating primary gout. Method: Self-made TongFengTang, modified based on syndrome differentiation and treatment, was used to treat 30 cases of gout, one dose per day, one course of treatment was seven days, curative effects and changes of blood uric acid were observed after one to three courses of treatment. Result: Total effective rate was 93.3%. Conclusion: Self-made TongFengTang shows significant effects in treating gout.

  7. 塞隆风湿酒对小鼠镇痛、抗寒的实验研究%Experimental Research Analgesic and resist-cool effects in Mice by Sai long Feng shi Wine

    Institute of Scientific and Technical Information of China (English)

    徐淑玲; 沈华; 王笑红

    2001-01-01

    To investigate Analgesic and resist-cool effects of sai long Feng shi wine in mice. Methods: Analgesic action of sai long Feng shi wine was obserated acetic acid-induced writhing test in mice. Anti-cool test was obserad in mice in -20℃ refrigerator.Results: Sai long feng shi wine can inhibit writing reaction and prolong the surrival time in cooled mice.%目的:观察塞隆风湿酒A、B及大小剂量对小鼠镇痛、抗寒冷作用影响。方法:采用昆明系小鼠灌胃塞隆酒A、B不同剂量,观察各组动物对醋酸致痛后发生扭体反应数及各组动物在-20℃低温条件下存活时间。结果:塞隆风湿酒A、B大小剂量对实验动物具有明显的镇痛及抗寒作用。

  8. Explanation Practice of Political Comment and Literature-On the Literary Criticism of FENG Xuefeng%政论与文艺的解释学实践——冯雪峰文学批评研究

    Institute of Scientific and Technical Information of China (English)

    刘志华; 周兴清

    2012-01-01

    以文艺政策为参照,以自身文艺领导干部的合法优势来确立文学批评标准和内容的规范,这是作为一个文学“批评”家的冯雪峰之文学批评“政论”特色的具体体现。另一方面,作为一个“文学”批评家,冯雪峰在典型、现实主义、艺术真实等问题上都有着一个艺术家所具有的艺术感受和认识。故其文学批评呈现出“政论与文艺”兼具的特色。%Analysing the literary policy as the reference, and establishing the standard and the content standard of the literary criticism by the leading cadre's legitimate superiority, which was the concrete manifestation of FENG Xuefeng' literary criticism "the political comment " characteristic when he is a literary "critics". On the other hand, FENG Xuefeng had some artistic feeling and understanding on the type, realism, art reality when he is a "literary" critics. So FENG Xuefeng' literary criticism showed the characteristics of both literature and political comment.

  9. Expression of ARE-binding proteins AUF1 and HuR in follicular adenoma and carcinoma of thyroid gland.

    Science.gov (United States)

    Trojanowicz, B; Sekulla, C; Dralle, H; Hoang-Vu, C

    2016-01-01

    Both adenylate-uridylate rich elements binding proteins AUF1 and HuR may participate in thyroid carcinoma progression. In this study we investigated the expression of both factors on a protein level with a special focus on follicular adenoma and follicular thyroid carcinoma. By employment of immunofluorescence and western blot on 68 thyroid tissues including 7 goiter, 16 follicular adenoma (4 adenomatous hyperplasia), 19 follicular thyroid carcinomas, 13 papillary thyroid carcinomas and 14 undifferentiated thyroid carcinomas we investigated protein expression of AUF1 and HuR. In addition to previous results we demonstrated that AUF1 and HuR are significantly up-regulated in carcinoma tissues as compared with follicular adenoma or goiter tissues. Furthermore, by evaluation of AUF1 or HuR expression, or combination of both proteins on total tissue lysates, we were able to demonstrate a significant difference between follicular adenoma and follicular thyroid carcinoma. Overexpression of AUF1 and HuR is a common finding observed in thyroid malignancy. Analysis of the tissues obtained by surgical resection as demonstrated in this study is comparable to a fine needle aspiration and in combination with AUF1/HuR immuno-analysis may support the conventional immunohistological investigations. The promising results of this study were performed on relatively small collective, but justify future development of a quick thyroid diagnostic test on larger cohort of the patients, especially for thyroid samples which are inadequate for histological examinations.

  10. Antonio Rodríguez Huéscar y José Ortega y Gasset: Tres cartas

    OpenAIRE

    Esteban Enguita, José Emilio; Lasaga Medina, José

    2015-01-01

    El objetivo principal de este artículo es comentar las cartas que se enviaron Antonio Rodríguez Huéscar y José Ortega y Gasset entre 1942 y 1943. Dichas cartas se incluyen en el artículo. The principal aim of this paper is to comment on the letters which Antonio Rodríguez Huéscar and José Ortega y Gasset sent each other between 1942 and 1943. The above said letters are included in the paper

  11. The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA

    OpenAIRE

    Lopez de Silanes, I.; Gorospe, M.; Taniguchi, H; Abdelmohsen, K; Srikantan, S.; Alaminos, M.; Berdasco, M.; Urdinguio, R. G.; Fraga, M. F.; Jacinto, F. V.; Esteller, M.

    2009-01-01

    The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression levels increase during tumorigenesis. Here, we present evidence that the expression of DNMT3b is post-transcriptionally regulated by HuR, an RNA-binding protein that stabilizes and/or modulates the translation of target mRNAs. The presence of a putative HuR-recognition motif in the DNMT3b 3???UTR pr...

  12. Comparison of the Debye–Hückel and the Mean Spherical Approximation Theories for Electrolyte Solutions

    DEFF Research Database (Denmark)

    Maribo-Mogensen, Bjørn; Kontogeorgis, Georgios M.; Thomsen, Kaj

    2012-01-01

    The thermodynamics of electrolyte solutions has been investigated by many scientists throughout the last century. While several theories have been presented, the most popular models for the electrostatic interactions are based on the Debye–Hückel and mean spherical approximation (MSA) theories....... In this paper we investigate the differences between the Debye–Hückel and the MSA theories, and comparisons of the numerical results for the Helmholtz energy and its derivatives with respect to temperature, volume and composition are presented. The investigation shows that the nonrestricted primitive MSA...

  13. Anti-solar differential rotation on the active sub-giant HU Virginis

    Science.gov (United States)

    Harutyunyan, G.; Strassmeier, K. G.; Künstler, A.; Carroll, T. A.; Weber, M.

    2016-08-01

    Context. Measuring surface differential rotation (DR) on different types of stars is important when characterizing the underlying stellar dynamo. It has been suggested that anti-solar DR laws can occur when strong meridional flows exist. Aims: We aim to investigate the differential surface rotation on the primary star of the RS CVn binary, HU Vir, by tracking its starspot distribution as a function of time. We also aim to recompute and update the values for several system parameters of the triple system HU Vir (close and wide orbits). Methods: Time-series high-resolution spectroscopy for four continuous months was obtained with the 1.2-m robotic STELLA telescope. Nine consecutive Doppler images were reconstructed from these data, using our line-profile inversion code iMap. An image cross-correlation method was applied to derive the surface differential-rotation law for HU Vir. New orbital elements for the close and the wide orbits were computed using our new STELLA radial velocities (RVs) combined with the RV data available in the literature. Photometric observations were performed with the Amadeus Automatic Photoelectric Telescope (APT), providing contemporaneous Johnson-Cousins V and I data for approximately 20 yrs. This data was used to determine the stellar rotation period and the active longitudes. Results: We confirm anti-solar DR with a surface shear parameter α of -0.029 ± 0.005 and -0.026 ± 0.009, using single-term and double-term differential rotation laws, respectively. These values are in good agreement with previously claimed results. The best fit is achieved assuming a solar-like double-term law with a lap time of ≈400 d. Our orbital solutions result in a period of 10.387678 ± 0.000003 days for the close orbit and 2726 ± 7 d (≈7.5 yr) for the wide orbit. A Lomb-Scarge (L-S) periodogram of the pre-whitened V-band data reveals a strong single peak providing a rotation period of 10.391 ± 0.008 d, well synchronized to the short orbit. Based on

  14. Feng Menglong's Thoughts on Literature and their Origin from Confucianism%冯梦龙的文学思想及其儒学根源

    Institute of Scientific and Technical Information of China (English)

    周群

    2000-01-01

    冯梦龙是一位致力于通俗文学的创作与批评的文学家。冯梦龙的尚俗主张主要是就文学体裁而言.冯梦龙的“情教”说,本源于儒家的学术思想.冯梦龙的文学直接承嗣了李贽,汤显祖、袁宏道等人的文学主张,并在俗文学领域中得到了新的拓展。%Feng Menglong is a writer and critic of popular literature. His thoughts on popularity of literary creation refer tc the genre of literature. His thoughts on literature's purpose of arousing emotions and education come from Confucianism. His literary creations are based on the development from his inheritance of the thoughts of Li Zhi, Tang Xianzu and Yuan Hongdao.

  15. “学雷锋”的假作文与德育教育反思%False composition of "Lei Feng" and the moral education reflection

    Institute of Scientific and Technical Information of China (English)

    舍娜莉

    2013-01-01

      “学雷锋”假作文折射出德育教育理念功利、内容空洞和方法单一等诸多问题,德国无私的德育理念、日本丰富的德育内容和美国宽松的德育方法给予我们很多思考和借鉴。培养诚实守信、脚踏实地、勇于担当的国民才是中国发展崛起的希望。%"Lei Feng" false composition reflects the moral education utilitarian, devoid of content and method for single and many other issues, methods of moral education moral education content of German selfless moral concept, Japan and the United States of America rich loose to give us a lot of thinking and reference. Honest culture, stand on solid ground, to bear the national is the Chinese development of the rise of hope.

  16. Isolation and characterization of a catalase gene "HuCAT3" from pitaya (Hylocereus undatus) and its expression under abiotic stress.

    Science.gov (United States)

    Nie, Qiong; Gao, Guo-Li; Fan, Qing-jie; Qiao, Guang; Wen, Xiao-Peng; Liu, Tao; Peng, Zhi-Jun; Cai, Yong-Qiang

    2015-05-25

    Abiotic stresses usually cause H2O2 accumulation, with harmful effects, in plants. Catalase may play a key protective role in plant cells by detoxifying this excess H2O2. Pitaya (Hylocereus undatus) shows broad ecological adaptation due to its high tolerance to abiotic stresses, e.g. drought, heat and poor soil. However, involvement of the pitaya catalase gene (HuCAT) in tolerance to abiotic stresses is unknown. In the present study, a full-length HuCAT3 cDNA (1870 bp) was isolated from pitaya based on our previous microarray data and RACE method. The cDNA sequence and deduced amino acid sequence shared 73-77% and 75-80% identity with other plant catalases, respectively. HuCAT3 contains conserved catalase family domain and catalytic sites. Pairwise comparison and phylogenetic analysis indicated that HuCAT3 is most similar to Eriobotrya japonica CAT, followed by Dimocarpus longan CAT and Nicotiana tabacum CAT1. Expression profile analysis demonstrated that HuCAT3 is mainly expressed in green cotyledons and mature stems, and was regulated by H2O2, drought, cold and salt stress, whereas, its expression patterns and maximum expression levels varied with stress types. HuCAT activity increased as exposure to the tested stresses, and the fluctuation of HuCAT activity was consistent with HuCAT3 mRNA abundance (except for 0.5 days upon drought stress). HuCAT3 mRNA elevations and HuCAT activities changes under cold stress were also in conformity with the cold tolerances among the four genotypes. The obtained results confirmed a major role of HuCAT3 in abiotic stress response of pitaya. This may prove useful in understanding pitaya's high tolerance to abiotic stresses at molecular level.

  17. Energy restriction affect liver development in Hu sheep ram lambs through Hippo signaling pathway.

    Science.gov (United States)

    Zhang, Ting-Ting; Zhang, Guo-Min; Jin, Yu-Hang; Guo, Yi-Xuan; Wang, Zhen; Fan, Yi-Xuan; El-Samahy, M A; Wang, Feng

    2017-09-02

    This study aimed to evaluate the effects of dietary energy restriction on postnatal liver development in Hu sheep ram lambs. A total of 16 ram lambs were randomly divided into two groups: 100% energy requirements diet and 55% energy requirements diet, which were fed for 75 d. Results showed that the final body and liver weights decreased with energy restriction (p Energy restriction caused a significant decrease in the levels of circulating insulin-like growth factor 1 (IGF1) and an increase in growth hormone secretion (p energy restriction, respectively (p energy restriction group (p energy restriction group (p energy restriction in ram lambs impairs liver development by increasing apoptosis, which may occur via the Hippo signaling pathway. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Flight Testing Surfaces Engineered for Mitigating Insect Adhesion on a Falcon HU-25C

    Science.gov (United States)

    Shanahan, Michelle; Wohl, Chris J.; Smith, Joseph G., Jr.; Connell, John W.; Siochi, Emilie J.; Doss, Jereme R.; Penner, Ronald K.

    2015-01-01

    Insect residue contamination on aircraft wings can decrease fuel efficiency in aircraft designed for natural laminar flow. Insect residues can cause a premature transition to turbulent flow, increasing fuel burn and making the aircraft less environmentally friendly. Surfaces, designed to minimize insect residue adhesion, were evaluated through flight testing on a Falcon HU-25C aircraft flown along the coast of Virginia and North Carolina. The surfaces were affixed to the wing leading edge and the aircraft remained at altitudes lower than 1000 feet throughout the flight to assure high insect density. The number of strikes on the engineered surfaces was compared to, and found to be lower than, untreated aluminum control surfaces flown concurrently. Optical profilometry was used to determine insect residue height and areal coverage. Differences in results between flight and laboratory tests suggest the importance of testing in realistic use environments to evaluate the effectiveness of engineered surface designs.

  19. The Curious Case of HU Aquarii - Dynamically Testing Proposed Planetary Systems

    CERN Document Server

    Horner, Jonathan; Marshall, Jonathan P; Tinney, Chris G; Butters, Oliver W

    2012-01-01

    In early 2011, the discovery of two planets moving on surprisingly extreme orbits around the eclipsing polar cataclysmic variable system HU Aquraii was announced based on variations in the timing of mutual eclipses between the two central stars. We perform a detailed dynamical analysis of the stability of the exoplanet system as proposed in that work, revealing that it is simply dynamically unfeasible. We then apply the latest rigorous methods used by the Anglo-Australian Planet Search to analyse radial velocity data to re-examine the data used to make the initial claim. Using that data, we arrive at a significantly different orbital solution for the proposed planets, which we then show through dynamical analysis to be equally unfeasible. Finally, we discuss the need for caution in linking eclipse-timing data for cataclysmic variables to the presence of planets, and suggest a more likely explanation for the observed signal.

  20. Congenital Pelger-Huët anomaly in a Danish/Swedish Farmdog: Case Report

    Directory of Open Access Journals (Sweden)

    Allison Robin W

    2011-03-01

    Full Text Available Abstract A 13 year old Danish/Swedish Farmdog from Denmark was evaluated in a veterinary clinic in Warsaw, Poland for evaluation of an orthopedic problem. Radiographs revealed spondylosis and degenerative vertebral disease, which responded to treatment with anti-inflammatory medications. A predominance of hyposegmented neutrophils and eosinophils containing condensed chromatin and normal cytoplasm were identified on a routine CBC. Follow-up blood film evaluations over the course of 12 months confirmed that the hyposegmented granulocytes persisted. The majority of neutrophils contained Grade 2 nuclei (slightly indented, and the mean nuclear score varied from 1.9 to 2.3. Pelger-Huët anomaly (PHA, presumably congenital, was diagnosed based on persistent hyposegmented granulocytes in the absence of an underlying cause for acquired PHA; genetically related dogs were unavailable for testing to confirm vertical transmission. To the authors' knowledge this is the first report of PHA in a Danish/Swedish Farmdog.

  1. Potential therapeutic use of antibodies directed towards HuIFN-gamma.

    Science.gov (United States)

    Froyen, G; Billiau, A

    1997-01-01

    IFN-gamma is an important regulator of immune responses and inflammation. Studies in animal models of inflammation, autoimmunity, cancer, transplant rejection and delayed-type hypersensitivity have indicated that administration of antibodies against IFN-gamma can prevent the occurrence of diseases or alleviate disease manifestations. Therefore, it is speculated that such antibodies may have therapeutical efficacy in human diseases. Since animal-derived antibodies are immunogenic in patients several strategies are being developed in order to reduce or abolish this human anti-mouse antibody (HAMA) response. In our laboratory, we have constructed a single-chain variable fragment (scFv) derived from a mouse antibody with neutralizing potential for human IFN-gamma. A scFv consists of only variable domains tethered together by a flexible linker. The scFv was demonstrated to neutralize the antiviral activity of HuIFN-gamma in vitro and therefore might be considered as a candidate for human therapy.

  2. La voz y la letra en "Un huésped", de Yoko Tawada

    Directory of Open Access Journals (Sweden)

    María Lucía Correa

    2009-05-01

    Full Text Available Los textos de Yoko Tawada, escritorajaponesa residente en Alemania,hacen presente el desgarramientode quien vive fuera de la lenguamaterna. Es una obra que se balanceasobre el límite entre loscódigos, donde nada hay evidente,donde la palabra parece tener vidapropia. Entre los textos que tomanesta extrañeza del lenguaje comosustrato, se encuentra el relato"Un huésped", cuya particular referenciaa la voz y a la letra comodos registros que se entrelazan yse repelen alternativamente, loconvierte en una valiosa fuente dereflexiones sobre lo oral, la voz, laletra, la lectura y la escritura.Palabras clave: Yoko Tawada, literaturajaponesa contemporánea, voz,letra, escritura.

  3. The SCID-hu mouse as a tool in immunotoxicological risk assessment: Effects of 2-acetyl-4(5)-tetrahydroxybutyl-imidazole (THI) and di-n-butyltin dichloride (DBTC) on the human thymus in SCID-hu mice

    NARCIS (Netherlands)

    Heer, C. de; Schuurman, H.J.; Houben, G.F.; Pieters, R.H.H.; Penninks, A.H.; Loveren, H. van

    1995-01-01

    SCID mice engrafted with human fetal thymus and liver tissue fragments (SCID-hu mice) are currently considered as a new tool in human immunotoxicological risk assessment. Testing of various immunotoxicants exerting thymotoxicity via different intrathymic target cell types is necessary for validation

  4. The complete chloroplast genome sequence of the relict woody plant Metasequoia glyptostroboides Hu et Cheng.

    Science.gov (United States)

    Chen, Jinhui; Hao, Zhaodong; Xu, Haibin; Yang, Liming; Liu, Guangxin; Sheng, Yu; Zheng, Chen; Zheng, Weiwei; Cheng, Tielong; Shi, Jisen

    2015-01-01

    Metasequoia glyptostroboides Hu et Cheng is the only species in the genus Metasequoia Miki ex Hu et Cheng, which belongs to the Cupressaceae family. There were around 10 species in the Metasequoia genus, which were widely spread across the Northern Hemisphere during the Cretaceous of the Mesozoic and in the Cenozoic. M. glyptostroboides is the only remaining representative of this genus. Here, we report the complete chloroplast (cp) genome sequence and the cp genomic features of M. glyptostroboides. The M. glyptostroboides cp genome is 131,887 bp in length, with a total of 117 genes comprised of 82 protein-coding genes, 31 tRNA genes and four rRNA genes. In this genome, 11 forward repeats, nine palindromic repeats, and 15 tandem repeats were detected. A total of 188 perfect microsatellites were detected through simple sequence repeat (SSR) analysis and these were distributed unevenly within the cp genome. Comparison of the cp genome structure and gene order to those of several other land plants indicated that a copy of the inverted repeat (IR) region, which was found to be IR region A (IRA), was lost in the M. glyptostroboides cp genome. The five most divergent and five most conserved genes were determined and further phylogenetic analysis was performed among plant species, especially for related species in conifers. Finally, phylogenetic analysis demonstrated that M. glyptostroboides is a sister species to Cryptomeria japonica (L. F.) D. Don and to Taiwania cryptomerioides Hayata. The complete cp genome sequence information of M. glyptostroboides will be great helpful for further investigations of this endemic relict woody plant and for in-depth understanding of the evolutionary history of the coniferous cp genomes, especially for the position of M. glyptostroboides in plant systematics and evolution.

  5. Antidepressant effects of curcumin and HU-211 coencapsulated solid lipid nanoparticles against corticosterone-induced cellular and animal models of major depression

    Science.gov (United States)

    He, Xiaolie; Zhu, Yanjing; Wang, Mei; Jing, Guoxin; Zhu, Rongrong; Wang, Shilong

    2016-01-01

    Major depression is a complex neuropsychiatric disorder with few treatment approaches. The use of nontargeted antidepressants induced many side effects with their low efficacy. A more precise targeting strategy is to develop nanotechnology-based drug delivery systems; hence, we employed solid lipid nanoparticles (SLNs) to encapsulate HU-211 and curcumin (Cur). The antidepressant effects of the dual-drug nanoparticles (Cur/SLNs-HU-211) for major depression treatment were investigated in corticosterone-induced cellular and animal models of major depression. Cur/SLNs-HU-211 can effectively protect PC12 cells from corticosterone-induced apoptosis and can release more dopamine, which may be associated with the higher uptake of Cur/SLNs-HU-211 shown by cellular uptake behavior analysis. Additionally, Cur/SLNs-HU-211 significantly reduced the immobility time in forced swim test, enhanced fall latency in rotarod test, and improved the level of dopamine in mice blood. Cur/SLNs-HU-211 can deliver more Cur to the brain and thus produce a significant increase in neurotransmitters level in brain tissue, especially in the hippocampus and striatum. The results of Western blot and immunofluorescence revealed that Cur/SLNs-HU-211 can significantly enhance the expression of CB1, p-MEK1, and p-ERK1/2. Our study suggests that Cur/SLNs-HU-211 may have great potential for major depression treatment. PMID:27757031

  6. HuD interacts with Bdnf mRNA and is essential for activity-induced BDNF synthesis in dendrites.

    Directory of Open Access Journals (Sweden)

    Filip Vanevski

    Full Text Available Highly specific activity-dependent neuronal responses are necessary for modulating synapses to facilitate learning and memory. We present evidence linking a number of important processes involved in regulating synaptic plasticity, suggesting a mechanistic pathway whereby activity-dependent signaling, likely through protein kinase C (PKC-mediated phosphorylation of HuD, can relieve basal repression of Bdnf mRNA translation in dendrites, allowing for increased TrkB signaling and synaptic remodeling. We demonstrate that the neuronal ELAV family of RNA binding proteins associates in vivo with several Bdnf mRNA isoforms present in the adult brain in an activity-dependent manner, and that one member, HuD, interacts directly with sequences in the long Bdnf 3' untranslated region (3'UTR and co-localizes with Bdnf mRNA in dendrites of hippocampal neurons. Activation of PKC leads to increased dendritic translation of mRNAs containing the long Bdnf 3'UTR, a process that is dependent on the presence of HuD and its phosphorylation at threonine residues 149 and/or 165. Thus, we found a direct effect of HuD on regulating translation of dendritic Bdnf mRNAs to mediate local and activity-dependent increases in dendritic BDNF synthesis.

  7. Genetic diversity and population structure in the narrow endemic Chinese walnut Juglans hopeiensis Hu: implications for conservation

    Science.gov (United States)

    Yiheng Hu; Meng Dang; Xiaojia Feng; Keith Woeste; Peng Zhao

    2017-01-01

    The conservation of narrow endemic species relies on accurate information regarding their population structure. Juglans hopeiensis Hu (Ma walnut), found only in Hebei province, Beijing, and Tianjin, China, is a threatened tree species valued commercially for its nut and wood. Sequences of two maternally inherited mitochondrial markers and two...

  8. The Preliminary Research on Rupture and Factors Affecting Rupturing of the Hoh Sai Hu Segment in Eastern Kunlun

    Institute of Scientific and Technical Information of China (English)

    Bai Yuzhu; Xu Jie; Zhou Qing; Zhou Bengang

    2012-01-01

    On November 14, 2001, an extraordinarily large earthquake (Ms8. 1) occurred on the Hoh Sai Hu segment of the Eastern Kunlun Fault, in the northern Qinghai-Tibetan Plateau. The seismogenic fault, the Hoh Sai Hu segment, is a left-lateral fault with a high slip rate in geological history, with the average slip rate reaching( 14. 8± 2. 8)mm/a since the late Pleistocene. Different slip rates of the Hoh Sai Hu segment can affect fault motion in the future. Therefore, this paper analyzes the effect of different slip rates and different initial friction coefficients on the fault plane of the Hoh Sai Hu segment of the eastern Kunlun Fault on the rupture behaviors of the fault. In this research, we apply the single degree of spring block model controlled by the rate- and state-dependent frictional constitutive laws. Using the fault dislocation model and based on ancient earthquake research, historical earthquakes data and the achievements of previous researchers, we obtained the parameters of this model. Through numerical simulation of the rupturing motion of the Hoh Sai Hu segment in the next 6500 years under different slip rates, we find that a faster annual slip rate will shorten the recurrence interval of the earthquake. For example, the earthquake recurrence interval is 2100a at a slip rate of 0. 014m/a, which agrees with previous research, but, the recurrence interval will be 1000 - 1500a and 2100 - 2500a, corresponding to the slip rates of 0. 018m/a and 0. 008m/a, respectively. The fault slip rate has no regular effect on the coseismic slip rate and fault displacement in an earthquake. The initial friction coefficient on the fault surface has an effect on the earthquake recurrence interval. A smaller initial friction coefficient will lengthen the earthquake recurrence interval. At the same time, the smaller initial friction coefficient will lead to larger slip rates and fault displacement when earthquakes occur.

  9. Striatal synaptic dysfunction and hippocampal plasticity deficits in the Hu97/18 mouse model of Huntington disease.

    Directory of Open Access Journals (Sweden)

    Karolina Kolodziejczyk

    Full Text Available Huntington disease (HD is a fatal neurodegenerative disorder caused by a CAG repeat expansion in the gene (HTT encoding the huntingtin protein (HTT. This mutation leads to multiple cellular and synaptic alterations that are mimicked in many current HD animal models. However, the most commonly used, well-characterized HD models do not accurately reproduce the genetics of human disease. Recently, a new 'humanized' mouse model, termed Hu97/18, has been developed that genetically recapitulates human HD, including two human HTT alleles, no mouse Hdh alleles and heterozygosity of the HD mutation. Previously, behavioral and neuropathological testing in Hu97/18 mice revealed many features of HD, yet no electrophysiological measures were employed to investigate possible synaptic alterations. Here, we describe electrophysiological changes in the striatum and hippocampus of the Hu97/18 mice. At 9 months of age, a stage when cognitive deficits are fully developed and motor dysfunction is also evident, Hu97/18 striatal spiny projection neurons (SPNs exhibited small changes in membrane properties and lower amplitude and frequency of spontaneous excitatory postsynaptic currents (sEPSCs; however, release probability from presynaptic terminals was unaltered. Strikingly, these mice also exhibited a profound deficiency in long-term potentiation (LTP at CA3-to-CA1 synapses. In contrast, at 6 months of age we found only subtle alterations in SPN synaptic transmission, while 3-month old animals did not display any electrophysiologically detectable changes in the striatum and CA1 LTP was intact. Together, these data reveal robust, progressive deficits in synaptic function and plasticity in Hu97/18 mice, consistent with previously reported behavioral abnormalities, and suggest an optimal age (9 months for future electrophysiological assessment in preclinical studies of HD.

  10. Alternativa terapeutica en la isquemia cerebral empleando la Eritropoyetina humana recombinante (rHu-EPO CIM como neuroprotector.

    Directory of Open Access Journals (Sweden)

    Iliana Sosa Testé

    2005-01-01

    Full Text Available Las enfermedades vasculares constituyen las terceras causas de muerte en Cuba. Su variante isquémica es causa de invalidez y perdida de la calidad de la vida. La búsqueda de agentes neuroprotectores es muy importante sí tenemos en cuenta que nuestra población está envejecida. Muchos son las razones por la que una molécula con propiedades neuroprotectoras en los modelos animales no tenga los resultados en ensayos clínicos en humanos. Este trabajo analiza y discute como mejorar estos aspectos. La Eritropoyetina humana recombinante (rHu EPO, conocido por su papel en la diferenciación del eritrocito, ha mostrado ser neuroprotector durante la isquemia cerebral en los modelos de animales adultos. De esta molécula se exponen los mecanismos de acción, que son las bases de los resultados positivos alcanzaron en las investigaciones preclínicas y los ensayos clínicos, que lo proponen como el neuroprotector ideal. Finalmente se comparan los resultados alcanzados por los autores en las investigaciones con la rHu EPO producida por el Centro Molecular de Inmunología de Cuba (CIM. El efecto de la aplicación de rHu EPO se evidenció por la disminución del edema cerebral, preservación de la conducta exploratoria y la disminución de la incidencia de señales clínicas de infarto cerebral y alteraciones patológicas. El grupo de resultados demostró las diferencias significantes entre los animales controles y tratados con el rHu EPO con los no tratados que sugieren que el rHu EPO debe ser considerado como una alternativa terapéutica contra el infarto cerebral en su fase aguda.

  11. A novel high throughput biochemical assay to evaluate the HuR protein-RNA complex formation.

    Directory of Open Access Journals (Sweden)

    Vito G D'Agostino

    Full Text Available The RNA binding protein HuR/ELAVL1 binds to AU-rich elements (AREs promoting the stabilization and translation of a number of mRNAs into the cytoplasm, dictating their fate. We applied the AlphaScreen technology using purified human HuR protein, expressed in a mammalian cell-based system, to characterize in vitro its binding performance towards a ssRNA probe whose sequence corresponds to the are present in TNFα 3' untranslated region. We optimized the method to titrate ligands and analyzed the kinetic in saturation binding and time course experiments, including competition assays. The method revealed to be a successful tool for determination of HuR binding kinetic parameters in the nanomolar range, with calculated Kd of 2.5±0.60 nM, k on of 2.76±0.56*10(6 M(-1 min(-1, and k off of 0.007±0.005 min(-1. We also tested the HuR-RNA complex formation by fluorescent probe-based RNA-EMSA. Moreover, in a 384-well plate format we obtained a Z-factor of 0.84 and an averaged coefficient of variation between controls of 8%, indicating that this biochemical assay fulfills criteria of robustness for a targeted screening approach. After a screening with 2000 small molecules and secondary verification with RNA-EMSA we identified mitoxantrone as an interfering compound with rHuR and TNFα probe complex formation. Notably, this tool has a large versatility and could be applied to other RNA Binding Proteins recognizing different RNA, DNA, or protein species. In addition, it opens new perspectives in the identification of small-molecule modulators of RNA binding proteins activity.

  12. On the Multiple Perspectives through which Feng Zikai Appreciated the Beauty of Folk Customs%论丰子恺民俗审美的多重视角

    Institute of Scientific and Technical Information of China (English)

    黄江平

    2012-01-01

    重视艺术与现实生活的密切联系,是丰子恺审美活动的逻辑起点。面对丰富的民俗生活和民俗事象,丰子恺从多角度、多层面展开民俗审美活动。在物质民俗审美中,丰子恺以对“人间烟火气”的盎然兴致,观察其家乡的衣食住行习俗,捕捉司空见惯的原生态民俗,刻画富于人情味和人情美的民俗生活。在艺术民俗审美中,不仅以玩具与花灯为代表的民间美术和民间工艺品是其民俗审美的重要内容,连日常生活中的叫卖声、小贩的招揽声、打桩的号子声也成了亲切而感人的“自然的音乐”,具有丰富的民俗文化价值。在信仰民俗审美中,他关注了家乡的佛道信仰、祖先崇拜及原始的动植物崇拜,通过对节日仪式的审美观照,展现了丰富的民间宗教信仰和原始巫术遗存。%Attaching importance to the close relationship between art and reality is the logical point of departure of Feng Zikai's aesthetic activities. Faced with a rich variety of folk customs and ways, Feng Zikai pursued his aesthetic appreciation of them from multi-angles and on multi-layers. When appreciating the material folk customs, he showed a keen interest in the everyday life of ordinary people, observing the daily habits of his country folks, taking note of pristine ways and customs which often escape an unobservant eye and depicting folk life that is filled with a human touch. When appreciating folk cus- toms of art, he not only studied typical arts and crafts like toys and festive lanterns, but also "the natural music" of crying one's ware, soliciting customers and chanting while piling that is rich in cultural value. When studying the folk customs of faith, he paid attention to the Buddhist and Taoist faiths of his country folks, their ancestor worship and their primitive wor- ship of animals and plants. Through an aesthetic appreciation of the festive rituals he revealed

  13. 孙维峰治疗痛风性关节炎经验%Sun Wei-feng's Experience of Treating Gouty Arthritis

    Institute of Scientific and Technical Information of China (English)

    劳贝妮

    2016-01-01

    Professor Sun Wei-feng thinks that gouty arthritis during the intermittent period and chronic phase are mainly due to yin deficiency of the liver and kidney leading to the spleen failing to activate,internal phlegmatic dampness,intermingled phlegm and blood stasis blocking in meridians,joints and the viscera.He emphasizes that "deficiency of the liver,spleen and kidney,intermingled phlegm and blood stasis" are the root of gouty arthritis during the intermittent period and chronic phase,so the treatment should focus on "purging turbidity and removing pathogenesis,dissolving the dampness and clearing away heat,activating blood circulation to remove blood stasis." He uses self-made (XZCBD) to treat it.He especially pays attention to prevention and regulation of patients with gouty arthritis.%孙维峰教授认为痛风性关节炎间歇期及慢性期的病因多为肝肾阴虚,脾失健运,痰湿内生,久则痰瘀互结,留滞经脉、关节、脏腑,强调“肝脾肾虚、痰瘀互结”为痛风的发病根源.孙教授认为痛风性关节炎间歇期及慢性期的治疗原则为“泄浊祛邪、化湿清热、活血化瘀”,自拟“泄浊除痹汤”治疗痛风间歇期及慢性期.孙维峰教授特别注重对痛风性关节炎患者生活上的预防调摄.

  14. Analgesic effect and mechanism of the three TCM-herbal drug-combination Tou Feng Yu pill on treatment of migraine.

    Science.gov (United States)

    Li, Jia-chuan; Shen, Xiao-fei; Meng, Xian-li; Zhang, Yi; Lai, Xian-rong

    2011-06-15

    It is well known that pain is one of the most important characteristics of migraine. Therefore, it is important and interesting to investigate the analgesic effect and mechanism of drugs which are used to treat migraine. Tou Feng Yu pill (TFY) is a traditional Chinese herbal medicine, consisting of the three Chinese herbal drugs Radix Angelicae dahuricae (Baizhi), Rhizome Ligustici (Chuanxiong) and Folium Camelliae sinensis (green tea) for the treatment of migraine. In this study, we found that TFY could significantly reduce the writhing times induced by acetic acid and licking foot response induced by formalin, and extend the writhing latent period. But the analgesic effect was not observed at hot-plate test. Meanwhile, experimental migrainous model induced by nitroglycerin was used to investigate the therapeutic effect of TFY. Compared with the control group, the levels of plasma calcitonin gene related to peptide (CGRP), serum nitric oxide (NO) and contents of brain dopamine (DA) in TFY administration groups were significantly decreased, and the levels of plasma endothelin (ET) and contents of brain 5-hydroxytryptamine (5-HT) and norepinephrine (NE) were remarkably increased, also the ratio of ET/NO was clearly corrected. Furthermore, the improving effect of behavior abnormality was observed in TFY administration rats. Meanwhile, isolated vascular ring test indicated that TFY had an significant effect on vasomotion, and antagonize vasospasm; moreover TFY also could increase cerebral blood flow (CBF) and reduce cerebrovascular resistance index (RI) in normal rabbits, which verified the effect of TFY on vasomotion and abnormal hemorheology. All the results indicate that TFY has an effective therapeutical action on migraine, which may be related to three aspects as following: firstly, adjusting the level of neurotransmitters, neuropeptides and vasoactive substances, relieving neurogenic inflammation; secondly, improving vasomotion, increase cerebral blood flow

  15. A new phantom for image quality, geometric destortion, and HU calibration in MSCT and CBCT

    Science.gov (United States)

    Voigt, Johannes M.; Blendl, Christian; Selbach, Markus; Uphoff, Clemens; Fiebich, Martin

    2012-03-01

    Flat panel cone-beam computed tomography (CBCT) is developing to the state-of-the-art technique in several medical disciplines such as dental and otorhinolaryngological imaging. Dental and otorhinolaryngological CBCT systems offer a variety of different field-of-view sizes from 6.0 to 17.0 cm. Standard phantoms are only designed for the use in multi-slices CT (MSCT) and there is no phantom which provides detail structures for all common characteristic values and Hounsfield calibration. In this study we present a new phantom specially designed for use with MSCT and CBCT systems providing detail structures for MTF, 3D MTF, NPS, SNR, geometric distortion and HU calibration. With this phantom you'll only need one acquisition for image quality investigation and assurance. Materials and methods: The phantom design is shown in figure 1. To investigate the practicability, the phantom was scanned using dedicated MSCT-scanners, 3D C-arms und digital volume tomographs. The acquired axial image stacks were analyzed using a dedicated computer program, which is provided as an ImageJ plugin. The MTF was compared to other methodologies such as a thin wire, a sphere or noise response [10, 13, 14]. The HU values were also computed using other common methods. Results: These results are similar to the results of others studies [10, 13, 14]. The method has proven to be stable and delivers comparable results to other methodologies such as using a thin wire. The NPS was calculated for all materials. Furthermore, CT numbers for all materials were computed and compared to the desired values. The measurement of geometric deformation has proven to be accurate. Conclusion: A unique feature of this phantom is to compute the geometric deformation of the 3D-volume image. This offers the chance to improve accuracy, e.g. in dental implant planning. Another convenient feature is that the phantom needs to be scanned only once with otorhinolaryngological volume tomographs to be fully displayed. It is

  16. {sup 89}Zr-huJ591 immuno-PET imaging in patients with advanced metastatic prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Pandit-Taskar, Neeta; Solomon, Stephen B.; Durack, Jeremy C.; Carrasquillo, Jorge A.; Lefkowitz, Robert A.; Osborne, Joseph R. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); O' Donoghue, Joseph A. [Memorial Sloan Kettering Cancer Center, Department of Medical Physics, New York, NY (United States); Beylergil, Volkan; Ruan, Shutian; Cheal, Sarah M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Lyashchenko, Serge [Memorial Sloan Kettering Cancer Center, Department of Radiochemistry and Molecular Imaging Probes Core, New York, NY (United States); Gonen, Mithat [Memorial Sloan Kettering Cancer Center, Department of Epidemiology and Biostatistics, New York, NY (United States); Lewis, Jason S. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Department of Radiochemistry and Molecular Imaging Probes Core, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Program in Molecular Pharmacology and Chemistry, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); Holland, Jason P. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Harvard Medical School, Department of Radiology of Massachusetts General Hospital, Boston, MA (United States); Reuter, Victor E. [Memorial Sloan Kettering Cancer Center, Department of Pathology, New York, NY (United States); Weill Cornell Medical College, Department of Pathology, New York, NY (United States); Loda, Massimo F. [Dana-Farber Cancer Institute, Boston, MA (United States); Broad Institute of Harvard and MIT, Cambridge, MA (United States); Smith-Jones, Peter M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Department of Psychiatry and Behavioral Science of Stony Brook University, Stony Brook, NY (United States); Weber, Wolfgang A.; Larson, Steven M. [Memorial Sloan Kettering Cancer Center, Department of Radiology, New York, NY (United States); Memorial Sloan Kettering Cancer Center, Program in Molecular Pharmacology and Chemistry, New York, NY (United States); Weill Cornell Medical College, Department of Radiology, New York, NY (United States); Bander, Neil H. [Memorial Sloan Kettering Cancer Center, Department of Surgery, New York, NY (United States); Weill Cornell Medical College, Department of Urology, New York, NY (United States); Scher, Howard I.; Morris, Michael J. [Memorial Sloan Kettering Cancer Center, Department of Medicine, New York, NY (United States); Weill Cornell Medical College, Department of Medicine, New York, NY (United States)

    2014-11-15

    Given the bone tropism of prostate cancer, conventional imaging modalities poorly identify or quantify metastatic disease. {sup 89}Zr-huJ591 positron emission tomography (PET) imaging was performed in patients with metastatic prostate cancer to analyze and validate this as an imaging biomarker for metastatic disease. The purpose of this initial study was to assess safety, biodistribution, normal organ dosimetry, and optimal imaging time post-injection for lesion detection. Ten patients with metastatic prostate cancer received 5 mCi of {sup 89}Zr-huJ591. Four whole-body scans with multiple whole-body count rate measurements and serum activity concentration measurements were obtained in all patients. Biodistribution, clearance, and lesion uptake by {sup 89}Zr-huJ591 immuno-PET imaging was analyzed and dosimetry was estimated using MIRD techniques. Initial assessment of lesion targeting of {sup 89}Zr-huJ591 was done. Optimal time for imaging post-injection was determined. The dose was well tolerated with mild chills and rigors seen in two patients. The clearance of {sup 89}Zr-huJ591 from serum was bi-exponential with biological half-lives of 7 ± 4.5 h (range 1.1-14 h) and 62 ± 13 h (range 51-89 h) for initial rapid and later slow phase. Whole-body biological clearance was 219 ± 48 h (range 153-317 h). The mean whole-body and liver residence time was 78.7 and 25.6 h, respectively. Dosimetric estimates to critical organs included liver 7.7 ± 1.5 cGy/mCi, renal cortex 3.5 ± 0.4 cGy/mCi, and bone marrow 1.2 ± 0.2 cGy/mCi. Optimal time for patient imaging after injection was 7 ± 1 days. Lesion targeting of bone or soft tissue was seen in all patients. Biopsies were performed in 8 patients for a total 12 lesions, all of which were histologically confirmed as metastatic prostate cancer. One biopsy-proven lesion was not positive on {sup 89}Zr-huJ591, while the remaining 11 lesions were {sup 89}Zr-huJ591 positive. Two biopsy-positive nodal lesions were noted only on

  17. Influence of acupuncturing Feng-fu acupoint on jaw-opening reflex of rats with tension-type headache%针刺风府穴对紧张型头痛大鼠张颌反射的影响

    Institute of Scientific and Technical Information of China (English)

    吴宏赟; 王希友; 王福文; 侯书伟

    2014-01-01

    Objective To explore the influence of acupuncturing Feng-fu acupoint on amplitude and latency of jaw-opening reflex (JOR) of rats with tension-type headache (TTH). Methods The TTH rat model was prepared by injecting ATP into rats' bilateral semispinalis capitis. 40 SPF-Wistar rats weighting 220-260 g, half male and half female, were divided into four groups: blank group, model group, Feng-fu acupoint group and non-acpoint group. Change of pain threshold and sensitivity of muscle and fascia in head and neck was observed by making quantitative analysis on JOR amplitude and latency of TTH model rats. Results No change of JOR's amplitude and latency was caused in blank group with NS injected into semispinalis capitis (P>0.05), JOR's amplitude was decreased in model group with ATP in-jected (P0.05), JOR's amplitude was increased and latency was shortened in Feng-fu acupoint group, JOR's amplitude was increased in non-acupoint group (P 0.05). Compared with model group, JOR's amplitude and latency of Feng-fu acupoint group were increased remarkably (P 0.05). Compared with non-acupoint group, JOR's amplitude of Feng-fu acupoint group was increased observably (P 0.05). Conclusion Acupuncturing Feng-fu acupoint could increase JOR's amplitude and extend its latency, in oth-er words, it can increase pain threshold and decrease sensitivity of muscle and fascia in head and neck.%目的:探索针刺风府穴对紧张型头痛大鼠张颌反射波幅和潜伏期的影响。方法采用大鼠双侧半棘肌注射三磷酸腺苷二钠注射液(ATP)的方法制备紧张型头痛大鼠模型。体重220~260 g的SPF级Wistar大鼠40只,雌雄各半,分为空白组、模型组、风府穴组和假穴组四组,通过定量分析紧张型头痛模型大鼠张颌反射波幅及潜伏期,反映头颈部皮肤肌肉筋膜痛阈及敏感性变化。结果空白组半棘肌注射生理盐水不引起张颌反射波幅及潜伏期变化(P>0.05);模型组半棘肌注射ATP导

  18. Mechanism of RNA binding protein HuR in regulating the activity of prostate cancer cells%RNA结合蛋白HuR调控前列腺癌细胞活力机制的研究

    Institute of Scientific and Technical Information of China (English)

    周宁; 程娟; 钟毅; 冉新建; 于文燕; 马琪

    2016-01-01

    目的 前列腺癌在我国的发病率及病死率逐年上升,但其发病机制尚未明确,本研究从转录后水平初步探讨RNA结合蛋白HuR参与调控前列腺癌细胞活力的机制.方法 免疫荧光检测前列腺增生细胞(BPH)及前列腺癌细胞(PC3)中HuR蛋白的定位,蛋白质印迹法检测HuR及环氧合酶-2(cyclooxygenase,COX-2)蛋白在细胞内的表达,MTT法检测细胞活力,脂质体转染法转染质粒及干扰片段,qPCR检测干扰效率,RNA-pull down实验验证HuR对COX-2的调控.结果 HuR在BPH细胞主要定位于细胞核,在PC3细胞中主要定位于胞质.PC3细胞中HuR表达水平为1.699±0.011,高于BPH细胞的0.654±0.028,差异有统计学意义,t=58.67,P<0.001.PC3细胞中过表达HuR后,转染空质粒组细胞活力为1.038±0.117,转染HuR表达质粒后细胞活力为1.838±0.057,差异有统计学意义,t=10.656,P<0.001.干扰HuR表达后,转染siNC组细胞活力为1.254±0.095,转染siHuR干扰片段组细胞活力为0.66±0.102,t=7.383,P=0.002;BPH组和PC3组COX-2表达水平分别为0.449±0.055和1.066±0.068,t=12.147,P<0.001;过表达COX-2后PC3细胞活力明显增加,转染空质粒组细胞活力为1.296±0.114,转染COX-2表达质粒后细胞活力为1.954±0.062,t=18.062,P<0.001.干扰COX-2表达后,PC3细胞活力明显降低,转染siNC组细胞活力为1.233±0.145,转染siCOX-2干扰片段后细胞活力为0.661±0.096,t=5.688,P=0.005.HuR可以结合于COX-2的3′-UTR并上调COX-2蛋白表达,转染空质粒组COX-2蛋白表达水平为0.638±0.067,转染HuR表达质粒组为1.703±0.022,t=26.26,P<0.001.结论 HuR参与调控前列腺癌细胞活力可能是通过上调COX-2表达而实现.

  19. 冯友兰伦理思想的精神特质与理论旨趣%On the spiritual characteristics and theoretical purport of Feng Youlan’s ethics thought

    Institute of Scientific and Technical Information of China (English)

    张克政; 范鹏

    2014-01-01

    Mr. Feng Youlan is an important ethicists in modern China. The traditional Confucian ethics is based the theoretical access which is highlighting human rational and self-conscious, looking for the inner world and meaning-world on the moralism theory of human nature, but Mr. Feng Youlan is different from traditional Confucian, he based the theoretical access on the rationalism theory of human nature, and constructed an ethics system which regards rationalism as its spiritual characteristic. To carry forward human rational spirit is not the final end-result of Mr. Feng Youlan’s ethics system, its theoretical purport expected people to use their reason, so as to continuously improve the spiritual realm, and shape the ideal personality that has extremely brilliant realm that accords with the Golden Mean, and properly settles self , finally.%冯友兰是我国现代一位重要的伦理学家。与传统儒家伦理学将彰显理性自觉,追求精神、意义世界的理路建立在道德人性论基础上不同,冯友兰从理性人性论这一理论进路,建构了以理性主义为精神特质的伦理思想体系。开明人的理性精神并不是冯友兰伦理思想体系的最终归宿,其理论旨趣是期望人们运用理性,不断提高精神境界,最终成就“极高明而道中庸”的理想人格,以妥善安顿自身。

  20. Anti-tradition and Tradition:Feng Yuanjun’ s Pain of “Love”---A Case Study of Feng Yuanjun’s Novel Writing in the 1920s%反传统与传统:冯沅君的“爱情”之痛--以冯沅君1920年代的小说创作为中心

    Institute of Scientific and Technical Information of China (English)

    杨华丽

    2013-01-01

    In her works written during the May 4th Movement, Feng Yuanjun, a female writer of new litera-ture, who has contributed three novel anthologies-Juan Shi, Chun Hen and Jie Hui-focuses on the heroine’ s pain due to her hesitation, indecision and struggle between her parent-child love and love for lovers. The destiny of the heroine’ s painful struggle in “love” is consistent with the conflict between the authoress’ rebellion under the impact of new ideas and her conversion because of her love for her mother. The vacillation between rebellion and attachment reflects exactly the plight of female intellectuals of the May 4 th Movement who have just made an appear-ance in society in the period of transition. Judged from the second and the third sections of her Tao Sha, it is ra-tional and inevitable that Feng Yuanjun should depict her pain of “love” in a highly autobiographic manner.%为“五四”文坛贡献了《卷葹》、《春痕》、《劫灰》这三部小说集的新文学女作家冯沅君,在此期小说中重点刻绘了女主人公在亲子之爱与情人之爱之间犹豫、彷徨、挣扎的痛苦情状。女主人公在“爱情”中痛苦挣扎的命运和作者自身因受新思潮的影响而具有的反叛性、因母亲之爱而具有的皈依性之间的冲突正相吻合。这种在反叛与眷恋之间的左右摇摆,拔心不死,正反应了“五四”一代刚刚浮出历史地表的女性知识分子在过渡时期的艰难处境。而从冯沅君的《淘沙》(二)(三)来看,她以自叙传色彩浓厚的方式来抒写自己的“爱情”之痛,乃是一种有着理性认知背景的必然。

  1. Detection of neutralizing antibodies to erythropoietin by inhibition of rHuEPO-stimulated EGR1 gene expression in the UT-7/EPO cell line.

    Science.gov (United States)

    Ferguson, Jackie; Bird, Chris; Wadhwa, Meenu; Burns, Chris

    2013-01-31

    Recombinant erythropoietin (rHuEPO) is used extensively to treat anaemia associated with chronic kidney disease. However, the development of neutralizing antibodies (NAbs) to rHuEPO can result in the development of antibody-mediated pure red cell aplasia (PRCA). The detection of NAb in patient sera by in vitro bioassay relies on the inhibition of a cellular response to rHuEPO. Current bioassays for rHuEPO measure proliferation in responsive cell lines such as the erythroleukaemic cell lines, UT-7 and UT-7/EPO, the latter sensitized to EPO. Using these cell lines, we show the dose-responsive induction of both PIM1 and EGR1 gene expression in UT-7 cells and of EGR1 in UT-7/EPO cells. The expression of EGR1 in UT-7/EPO cells in response to rHuEPO was comparable to the proliferative response measured by (3)H-thymidine incorporation and could be inhibited by serum from a patient with NAb-mediated PRCA in a dilution-dependent manner. Bioassays based on the induction of endogenous gene expression are comparable to current bioassays but are considerably quicker given that incubation time is decreased from 2-3 days to 50 min. Measurement of EGR1 gene expression in response to rHuEPO in UT-7/EPO cells offers a rapid, non-radioactive and automatable alternative to current assays for the detection of rHuEPO NAbs.

  2. 东风EQ1166G车制动系统介绍及常见故障排除%Introduction of DongFeng EQ1166G brake system and its common trouble shooting.

    Institute of Scientific and Technical Information of China (English)

    蒋显斌; 王涛

    2001-01-01

    介绍了EQ1166G车制动系统部分制动阀类的功用,针对制动系统较容易出现的故障,进行分析判断,找出引起故障的原因,结合实际提出故障排除的具体方法。%Use of brake valves for brake system in DongFeng EQ1166G isintroduced and its common troubles are analyzed、 diagnosed and removed

  3. 一战期间冯国璋对中德关系的态度转变探析%Analysis of FENG Guo-zhang's Changes in Attitude toward Sino-German Relation during the First World War

    Institute of Scientific and Technical Information of China (English)

    彭贺超

    2011-01-01

    During the First World War,China's policy for German went through three stages:neutrality,protesting and breaking off relations,and declaring war.In the last two stages,FENG Guo-zhang's attitude towards Sino-German relation changed twice.In order to mediate the dispute between the Department of State and the Office of the President,FENG Guo-zhang's attitude changed from opposing to fighting against and breaking with German into approving of breaking off relations.At the third stage,FENG Guo-zhang's attitude shifted from being against going to war into strongly advocating participating in the war,which was done to some extent out of the consideration of national long-term interests.In comparison with the first change,the second one is more aggressive.FENG Guo-zhang's two changes in attitude objectively promoted the successful implementation of the policy of protesting and breaking off relations,and declaring war on German.%一战期间,中国对德政策经历中立、抗议绝交、宣战三个阶段,在后两阶段冯国璋态度历经两度转变。冯国璋从调解府院之争的角度出发,由反对抗德绝交转变为赞同绝交;在第三阶段,冯国璋由反对参战到力主参战的转变,一定程度上出于国家长远利益的考量。相较前者而论,冯国璋后者态度转变更具积极进取色彩。冯国璋态度的两次转变客观上催化了北京政府的抗德绝交政策、对德奥宣战政策的顺利实施。

  4. Testing Hu-Sawicki f(R) gravity with the Effective Field Theory approach

    CERN Document Server

    Hu, Bin; Rizzato, Matteo; Silvestri, Alessandra

    2016-01-01

    We show how to fully map a specific model of modified gravity into the Einstein-Boltzmann solver EFTCAMB. This approach consists in few steps and allows to obtain the cosmological phenomenology of a model with minimal effort. We discuss all these steps, from the solution of the dynamical equations for the cosmological background of the model to the use of the mapping relations to cast the model into the effective field theory language and use the latter to solve for perturbations. We choose the Hu-Sawicki f(R) model of gravity as our working example. After solving the background and performing the mapping, we interface the algorithm with EFTCAMB and take advantage of the effective field theory framework to integrate the full dynamics of linear perturbations, returning all quantities needed to accurately compare the model with observations. We discuss some observational signatures of this model, focusing on the linear growth of cosmic structures. In particular we present the behavior of $f\\sigma_8$ and $E_G$ t...

  5. La huída en la obra de Mario Vargas Llosa

    Directory of Open Access Journals (Sweden)

    German Arce Ross

    Full Text Available Se trata de poner en perspectiva la mayor parte de novelas de Mario Vargas Llosa en función del tema de la huída, partiendo del hecho que é1 solicitó la nacionalidad Española inmediatamente después de haber perdido las elecciones para la Presidencia del Perú y de haber escrito El pez en el agua. Nuestro análisis intenta demostrar la importancia de la figura paterna – principalmente en el sentido de su ausencia y de sus bruscas reapariciones – en la cristalización sintomática del tema de la fuga. Es por eso que una analogía con la posición fóbica, en relación al padre o a sus substitutos, nos ha parecido pertinente. Teniendo como pivot el análisis de la forma, del contexto y del contenido de su libro de memorias, este trabajo definiría así las bases de una contribución literaria de la obra de Vargas Llosa a la clinica de la fobia.

  6. Systematics of a kleptoplastidal dinoflagellate, Gymnodinium eucyaneum Hu (Dinophyceae, and its cryptomonad endosymbiont.

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    Shuang Xia

    Full Text Available New specimens of the kleptoplastidal dinoflagellate Gymnodinium eucyaneum Hu were collected in China. We investigated the systematics of the dinoflagellate and the origin of its endosymbiont based on light morphology and phylogenetic analyses using multiple DNA sequences. Cells were dorsoventrally flattened with a sharply acute hypocone and a hemispherical epicone. The confusion between G. eucyaneum and G. acidotum Nygaard still needs to be resolved. We found that the hypocone was conspicuously larger than the epicone in most G. eucyaneum cells, which differed from G. acidotum, but there were a few cells whose hypocone and epicone were of nearly the same size. In addition, there was only one site difference in the partial nuclear LSU rDNA sequences of a sample from Japan given the name G. acidotum and G. eucyaneum in the present study, which suggest that G. eucyaneum may be a synonym of G. acidotum. Spectroscopic analyses and phylogenetic analyses based on nucleomorph SSU rDNA sequences and chloroplast 23 s rDNA sequences suggested that the endosymbiont of G. eucyaneum was derived from Chroomonas (Cryptophyta, and that it was most closely related to C. coerulea Skuja. Moreover, the newly reported kleptoplastidal dinoflagellates G. myriopyrenoides and G. eucyaneum in our study were very similar, and the taxonomy of kleptoplastidal dinoflagellates was discussed.

  7. Screening of a xylanase clone from a fosmid library of rumen microbiota in Hu sheep.

    Science.gov (United States)

    Wang, Jiakun; Sun, Zhongyuan; Zhou, Yan; Wang, Qian; Ye, Jun'an; Chen, Zhenming; Liu, Jianxin

    2012-01-01

    The glycosyl hydrolase family 11, which is responsible for carbohydrate metabolism, was identified in the open reading frame (ORF) 6 of a xylanase positive clone from a fosmid library of rumen microbiota of Hu sheep. A BLASTP search of GenBank revealed that ORF6 encoded a 355-amino acid putative endoxylanase, having 61% similarity (e(-73)) to endo-1,4-β-xylanase of Fibrobacter succinogenes S85 (YP_003250510.1). Predicted with the SWISS-MODEL, there were two separate β-sandwich clusters linked with a high serine containing linker in ORF6. The N-terminal β-sandwich is a novel endoxylanase of the glycosyl hydrolase family 11 with a specific activity of 1150.00 U/mg. The optimal pH and temperature for this enzyme were shown to be pH 5.0 and 50°C, respectively. The C-terminal helped increase the stability of the xylanase but decreased the activity to some degree. The C-terminal β-sandwich could bind avicel, but no conserved domain could be found. It may be a novel carbohydrate-binding module.

  8. Managing Stability in the Taiwan Strait: Non-Military Policy towards Taiwan under Hu Jintao

    Directory of Open Access Journals (Sweden)

    Wu-ueh Chang

    2009-10-01

    Full Text Available China’s Taiwan policy has been one of coupling intimidation (the “stick” approach with coercion (the “carrot” approach, a policy mix which, in the near term, is not likely to change, as is evidenced by the passage of the “Anti-Secession Law” in March, 2005. However, under Hu Jintao, the focus has been on pragmatism. The warm atmosphere that presently reigns in the Taiwan Strait area is unprecedented. Further talks are expected before the two cross-Strait leaders are slated to step down, simultaneously, in 2012. An era of reconciliation and negotiations has dawned. For the first time there is consensus regarding norms of interaction between the two sides. Cross-Strait relations have stabilized after years of tumult. More open, stable and predictable cross-Strait relations are in the interests of both sides. Difficulties surely lie ahead, but they will be dealt with in a different manner than what has been witnessed in the past.

  9. Detection of a planetary system orbiting the eclipsing polar HU Aqr

    CERN Document Server

    Qian, S -B; Liao, W -P; Li, L -J; Zhu, L -Y; Dai, Z -B; He, J -J; Zhao, E -G; Zhang, J; Li, K

    2011-01-01

    Using the precise times of mid-egress of the eclipsing polar HU Aqr, we discovered that this polar is orbited by two or more giant planets. The two planets detected so far have masses of at least 5.9 and 4.5\\,M_{Jup}. Their respective distances from the polar are 3.6 AU and 5.4 AU with periods of 6.54 and 11.96 years, respectively. The observed rate of period decrease derived from the downward parabolic change in O-C curve is a factor 15 larger than the value expected for gravitational radiation. This indicates that it may be only a part of a long-period cyclic variation, revealing the presence of one more planet. It is interesting to note that the two detected circumbinary planets follow the Titus-Bode law of solar planets with n=5 and 6. We estimate that another 10 years of observations will reveal the presence of the predicted third planet.

  10. Construction of a eukaryotic expression plasmid pcDNA3.1-HuR-FLAG and its transient expression in NIH3T3 cells

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    Tao LI

    2011-04-01

    Full Text Available Objective To construct a eukaryotic expression vector for HuR and analyze its expression and biological function in NIH3T3 cells.Methods The total RNA was extracted from NIH3T3 cells and reverse transcribed to cDNAs.The coding region sequence of mouse HuR was then amplified by PCR and subcloned into the pcDNA3.1-FLAG plasmid.The recombinant plasmid pcDNA3.1-HuR-FLAG was verified by PCR and restriction endonuclease analysis,confirmed by DNA sequence analysis,and then transiently transfected into NIH3T3 cells with Lipofectamine LTX.The expression of HuR protein was determined by Western blotting,and the mRNA level of HuR and DUSP1 were analyzed by using real-time PCR.Result The recombinant plasmid pcDNA3.1-HuR-FLAG was correctly constructed.Twenty-four hours after transfection of the recombinant plasmid into NIH3T3 cells,the fusion protein was found to have highly expressed in the cells as revealed by Western blotting.Real-time PCR results detected that the over-expression of HuR could up-regulate the expression of DUSP1.Conclusion The eukaryotic expression vector for HuR-FLAG fusion protein has been successfully constructed and transiently expressed in NIH3T3 cells.It can be used in further analysis of the posttranscriptional regulation of DUSP1 by HuR in cancer cells.

  11. Massive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips.

    Energy Technology Data Exchange (ETDEWEB)

    Krylov, A. S.; Zasedateleva, O. A.; Prokopenko, D. V.; Rouviere-Yaniv, J.; Mirzabekov, A. D.; Biochip Technology Center; Engelhardt Inst. of Molecular Biology; Inst. de Biologie Physico-Chimique

    2001-06-15

    A generic hexadeoxyribonucleotide microchip has been applied to test the DNA-binding properties of HU histone-like bacterial protein, which is known to have a low sequence specificity. All 4096 hexamers flanked within 8mers by degenerate bases at both the 3'- and 5'-ends were immobilized within the 100 x 100 x 20 mm polyacrylamide gel pads of the microchip. Single-stranded immobilized oligonucleotides were converted in some experiments to the double-stranded form by hybridization with a specified mixture of 8mers. The DNA interaction with HU was characterized by three type of measurements: (i) binding of FITC-labeled HU to microchip oligonucleotides; (ii) melting curves of complexes of labeled HU with single-stranded microchip oligonucleotides; (iii) the effect of HU binding on melting curves of microchip double-stranded DNA labeled with another fluorescent dye, Texas Red. Large numbers of measurements of these parameters were carried out in parallel for all or many generic microchip elements in real time with a multi-wavelength fluorescence microscope. Statistical analysis of these data suggests some preference for HU binding to G/C-rich single-stranded oligonucleotides. HU complexes with double-stranded microchip 8mers can be divided into two groups in which HU binding either increased the melting temperature (T{sub m}) of duplexes or decreased it. The stabilized duplexes showed some preference for presence of the sequence motifs AAG, AGA and AAGA. In the second type of complex, enriched with A/T base pairs, the destabilization effect was higher for longer stretches of A/T duplexes. Binding of HU to labeled duplexes in the second type of complex caused some decrease in fluorescence. This decrease also correlates with the higher A/T content and lower T{sub m}. The results demonstrate that generic microchips could be an efficient approach in analysis of sequence specificity of proteins.

  12. Antidepressant effects of curcumin and HU-211 coencapsulated solid lipid nanoparticles against corticosterone-induced cellular and animal models of major depression

    Directory of Open Access Journals (Sweden)

    He XL

    2016-10-01

    Full Text Available Xiaolie He,* Yanjing Zhu,* Mei Wang, Guoxin Jing, Rongrong Zhu, Shilong Wang Research Center for Translational Medicine at East Hospital, School of Life Science and Technology, Tongji University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Major depression is a complex neuropsychiatric disorder with few treatment approaches. The use of nontargeted antidepressants induced many side effects with their low efficacy. A more precise targeting strategy is to develop nanotechnology-based drug delivery systems; hence, we employed solid lipid nanoparticles (SLNs to encapsulate HU-211 and curcumin (Cur. The antidepressant effects of the dual-drug nanoparticles (Cur/SLNs-HU-211 for major depression treatment were investigated in corticosterone-induced cellular and animal models of major depression. Cur/SLNs-HU-211 can effectively protect PC12 cells from corticosterone-induced apoptosis and can release more dopamine, which may be associated with the higher uptake of Cur/SLNs-HU-211 shown by cellular uptake behavior analysis. Additionally, Cur/SLNs-HU-211 significantly reduced the immobility time in forced swim test, enhanced fall latency in rotarod test, and improved the level of dopamine in mice blood. Cur/SLNs-HU-211 can deliver more Cur to the brain and thus produce a significant increase in neurotransmitters level in brain tissue, especially in the hippocampus and striatum. The results of Western blot and immunofluorescence revealed that Cur/SLNs-HU-211 can significantly enhance the expression of CB1, p-MEK1, and p-ERK1/2. Our study suggests that Cur/SLNs-HU-211 may have great potential for major depression treatment. Keywords: major depression, curcumin, HU-211, solid lipid nanoparticles, dopamine

  13. Effect of various factors and substrates on the growth of a human hepatoblastoma cell line, HuH-6 in a serum-free medium.

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    Tokiwa,Takayoshi

    1989-12-01

    Full Text Available The effect of various factors and substrates on the growth of a human hepatoblastoma cell line, HuH-6, which was inoculated at low density in a serum-free medium was examined. Several supplements were required to enhance cell growth of HuH-6. These included cholera toxin (CT, glucagon (Glu and selenium (Se. Type IV collagen (C-IV provided the most conductive environment tested for cell growth. These results suggest that CT, Glu, Se, and C-IV are important stimulators for the continuous growth of HuH-6 in a serum-free medium at low density.

  14. Identification of flavonoid diglycosides in several genotypes of asparagus from the Huétor-Tájar population variety.

    Science.gov (United States)

    Fuentes-Alventosa, José María; Rodríguez, Guillermo; Cermeño, Pedro; Jiménez, Ana; Guillén, Rafael; Fernández-Bolaños, Juan; Rodríguez-Arcos, Rocío

    2007-11-28

    The qualitative and quantitative composition of flavonoids from the Huétor-Tájar population variety of asparagus (commonly known as " triguero") was investigated. Flavonoids were analyzed by reversed-phase high-performance liquid chromatography-diode array detection (HPLC-DAD). Liquid chromatography-mass spectrometry (LC-MS) under identical HPLC conditions was used to verify the identities of the flavonoid glycosides from triguero asparagus. The quantities of asparagus flavonoids were calculated according to concentration curves constructed with authentic standards. Total flavonoid contents, calculated as the sum of individual compounds, were determined and ranged from 400 to 700 mg/kg fresh weight. The most abundant was rutin, which represented 55-98% of the total flavonoid complement. Triguero asparagus were revealed to be an important source of not only quercetin derivatives but also kaempferol and isorhamnetin glycosides. Significant differences (p asparagus genotypes from the Huétor-Tájar population variety.

  15. METOD FOR ESTABLISHING FRAGILE MATRIXES OF GROUPS OF BUILDINGS IN SHIHEZI CITY AND ITS EARTHQUAKE DISASTER PREDICTIONSONG Li-jun1,TANG Li-hua1,YIN Li-feng1,HU Wei-hua1,%石河子市建筑物群体易损性矩阵的建立方法及震害预测

    Institute of Scientific and Technical Information of China (English)

    宋立军; 唐丽华; 尹力峰; 胡伟华; 薛健; 邢佑宏; 苗军; 陈伟

    2001-01-01

    在前人建立的易损性矩阵方法的基础上,结合具体预测对象的实际,并根据普查资料统计出建筑物设防情况和现状,给出相应的权重,进行加权平均,建立具体预测对象群体建筑物的易损性矩阵.以石河子市为例,运用该方法给出了分类建筑物易损性矩阵及震害预测结果.

  16. HTLV-1 Infection and Neuropathogenesis in the Context of Rag1(-/-)γc(-/-) (RAG1-Hu) and BLT Mice.

    Science.gov (United States)

    Ginwala, Rashida; Caruso, Breanna; Khan, Zafar K; Pattekar, Ajinkya; Chew, Glen M; Corley, Michael J; Loonawat, Ronak; Jacobson, Steven; Sreedhar, Sreesha; Ndhlovu, Lishomwa C; Jain, Pooja

    2017-09-01

    To date, the lack of a suitable small animal model has hindered our understanding of Human T-cell lymphotropic virus (HTLV)-1 chronic infection and associated neuropathogenesis defined as HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The host immune response plays a critical role in the outcome of HTLV-1 infection, which could be better tested in the context of humanized (hu) mice. Thus, we employ here the Balb/c-Rag1(-/-)γc(-/-) or Rag1 as well as Bone marrow-Liver-Thymic (BLT) mouse models for engraftment of human CD34(+) hematopoietic stem cells. Flow cytometry and histological analyses confirmed reconstitution of Rag1 and BLT mice with human immune cells. Following HTLV-1 infection, proviral load (PVL) was detected in the blood of Rag-1 and BLT hu-mice as early as 2 weeks post-infection (wpi) with sustained elevation in the subsequent weeks followed by Tax expression. Additionally, infection was compared between adult and neonatal Rag1 mice with both PVL and Tax expression considerably higher in the adult Rag1 mice as compared to the neonates. Establishment of peripheral infection led to lymphocytic infiltration with concomitant Tax expression and resulting myelin disruption within the central nervous system of infected mice. In addition, up-regulation in the expression of several immune checkpoint mediators such as programmed cell death-1 (PD-1), T-cell Ig and ITIM domain (TIGIT), and T cell Ig and mucin domain-3 protein (Tim-3) were observed on CD8(+) T cells in various organs including the CNS of infected hu-mice. Collectively, these studies represent the first attempt to establish HTLV-1 neuropathogenesis in the context of Rag-1 and BLT hu-mice as potential novel tools for understanding HTLV-1 neuropathogenesis and testing of novel therapies such as immune checkpoint blockade in the amelioration of chronic HTLV-1 infection.

  17. Generalized Split-Window Algorithm for Estimate of Land Surface Temperature from Chinese Geostationary FengYun Meteorological Satellite (FY-2C Data

    Directory of Open Access Journals (Sweden)

    Jun Xia

    2008-02-01

    Full Text Available On the basis of the radiative transfer theory, this paper addressed the estimate ofLand Surface Temperature (LST from the Chinese first operational geostationarymeteorological satellite-FengYun-2C (FY-2C data in two thermal infrared channels (IR1,10.3-11.3 μ m and IR2, 11.5-12.5 μ m , using the Generalized Split-Window (GSWalgorithm proposed by Wan and Dozier (1996. The coefficients in the GSW algorithmcorresponding to a series of overlapping ranging of the mean emissivity, the atmosphericWater Vapor Content (WVC, and the LST were derived using a statistical regressionmethod from the numerical values simulated with an accurate atmospheric radiativetransfer model MODTRAN 4 over a wide range of atmospheric and surface conditions.The simulation analysis showed that the LST could be estimated by the GSW algorithmwith the Root Mean Square Error (RMSE less than 1 K for the sub-ranges with theViewing Zenith Angle (VZA less than 30° or for the sub-rangs with VZA less than 60°and the atmospheric WVC less than 3.5 g/cm2 provided that the Land Surface Emissivities(LSEs are known. In order to determine the range for the optimum coefficients of theGSW algorithm, the LSEs could be derived from the data in MODIS channels 31 and 32 provided by MODIS/Terra LST product MOD11B1, or be estimated either according tothe land surface classification or using the method proposed by Jiang et al. (2006; and theWVC could be obtained from MODIS total precipitable water product MOD05, or beretrieved using Li et al.’ method (2003. The sensitivity and error analyses in term of theuncertainty of the LSE and WVC as well as the instrumental noise were performed. Inaddition, in order to compare the different formulations of the split-window algorithms,several recently proposed split-window algorithms were used to estimate the LST with thesame simulated FY-2C data. The result of the intercomparsion showed that most of thealgorithms give

  18. 志愿者精神对雷锋精神的传承和发展%Volunteerism: Tradition and Development of Spirit of Lei Feng

    Institute of Scientific and Technical Information of China (English)

    苏艳丽

    2012-01-01

    志愿者精神与雷锋精神一脉相承,两种精神拥有许多共核,如心存犬爱、奉献为乐等等。但二者也有明显不同。与雷锋的公而忘私相比,当今的志愿者在利他的同时,也尊重自身的意愿和权利,并以付出收获成长。从“毫不利己、专门利人”的雷锋精神到“赠人玫瑰、手有余香”的志愿者群体,服务理念的发展变化体现了以人为本,体现了时代进步,也体现了公民意识的觉醒。本文还提出,志愿服务工作和公民道德建设宜着眼于基础性的道德层面,引导公众从力所能及的善举做起,逐步提升社会文明的整体水平,并要警惕以崇高的名义陷入道德偏执。%Volunteerism and the spirit of Lei Feng have much in common in that they embrace the values such as love and devotion. However, the qualities of volunteerism such as advocates of their own rights and willingness also set them apart. The shift from altruism to the extreme to offering services and improving oneself reflect the progress of our times such as the recognition of self-awareness. It is pointed out volunteerism and ethics development should start with the fundamental level and basic services people can offer to the public to promote the social progress. At the same time, we should shun the trap of moral bigotry in the name of sublime.

  19. Comment on "Tropospheric temperature response to stratospheric ozone recovery in the 21st century" by Hu et al. (2011

    Directory of Open Access Journals (Sweden)

    C. McLandress

    2012-03-01

    Full Text Available In a recent paper Hu et al. (2011 suggest that the recovery of stratospheric ozone during the first half of this century will significantly enhance free tropospheric and surface warming caused by the anthropogenic increase of greenhouse gases, with the effects being most pronounced in Northern Hemisphere middle and high latitudes. These surprising results are based on a multi-model analysis of CMIP3 model simulations with and without prescribed stratospheric ozone recovery. Hu et al. suggest that in order to properly quantify the tropospheric and surface temperature response to stratospheric ozone recovery, it is necessary to run coupled atmosphere-ocean climate models with stratospheric ozone chemistry. The results of such an experiment are presented here, using a state-of-the-art chemistry-climate model coupled to a three-dimensional ocean model. In contrast to Hu et al., we find a much smaller Northern Hemisphere tropospheric temperature response to ozone recovery, which is of opposite sign. We suggest that their result is an artifact of the incomplete removal of the large effect of greenhouse gas warming between the two different sets of models.

  20. Cannabinoid HU210 protects isolated rat stomach against impairment caused by serum of rats with experimental acute pancreatitis.

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    Ming-hua Cao

    Full Text Available Acute pancreatitis (AP, especially severe acute pancreatitis often causes extra-pancreatic complications, such as acute gastrointestinal mucosal lesion (AGML which is accompanied by a considerably high mortality, yet the pathogenesis of AP-induced AGML is still not fully understood. In this report, we investigated the alterations of serum components and gastric endocrine and exocrine functions in rats with experimental acute pancreatitis, and studied the possible contributions of these alterations in the pathogenesis of AGML. In addition, we explored the intervention effects of cannabinoid receptor agonist HU210 and antagonist AM251 on isolated and serum-perfused rat stomach. Our results showed that the AGML occurred after 5 h of AP replication, and the body homeostasis was disturbed in AP rat, with increased levels of pancreatic enzymes, lipopolysaccharide (LPS, proinflammtory cytokines and chemokines in the blood, and an imbalance of the gastric secretion function. Perfusing the isolated rat stomach with the AP rat serum caused morphological changes in the stomach, accompanied with a significant increment of pepsin and [H+] release, and increased gastrin and decreased somatostatin secretion. HU210 reversed the AP-serum-induced rat pathological alterations, including the reversal of transformation of the gastric morphology to certain degree. The results from this study prove that the inflammatory responses and the imbalance of the gastric secretion during the development of AP are responsible for the pathogenesis of AGML, and suggest the therapeutic potential of HU210 for AGML associated with acute pancreatitis.

  1. Alginate hydrogel protects encapsulated hepatic HuH-7 cells against hepatitis C virus and other viral infections.

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    Nhu-Mai Tran

    Full Text Available Cell microencapsulation in alginate hydrogel has shown interesting applications in regenerative medicine and the biomedical field through implantation of encapsulated tissue or for bioartificial organ development. Although alginate solution is known to have low antiviral activity, the same property regarding alginate gel has not yet been studied. The aim of this work is to investigate the potential protective effect of alginate encapsulation against hepatitis C virus (HCV infection for a hepatic cell line (HuH-7 normally permissive to the virus. Our results showed that alginate hydrogel protects HuH-7 cells against HCV when the supernatant was loaded with HCV. In addition, alginate hydrogel blocked HCV particle release out of the beads when the HuH-7 cells were previously infected and encapsulated. There was evidence of interaction between the molecules of alginate hydrogel and HCV, which was dose- and incubation time-dependent. The protective efficiency of alginate hydrogel towards HCV infection was confirmed against a variety of viruses, whether or not they were enveloped. This promising interaction between an alginate matrix and viruses, whose chemical mechanisms are discussed, is of great interest for further medical therapeutic applications based on tissue engineering.

  2. Chemopreventive apigenin controls UVB-induced cutaneous proliferation and angiogenesis through HuR and thrombospondin-1.

    Science.gov (United States)

    Tong, Xin; Mirzoeva, Salida; Veliceasa, Dorina; Bridgeman, Bryan B; Fitchev, Philip; Cornwell, Mona L; Crawford, Susan E; Pelling, Jill C; Volpert, Olga V

    2014-11-30

    Plant flavonoid apigenin prevents and inhibits UVB-induced carcinogenesis in the skin and has strong anti-proliferative and anti-angiogenic properties. Here we identify mechanisms, by which apigenin controls these oncogenic events. We show that apigenin acts, at least in part, via endogenous angiogenesis inhibitor, thrombospondin-1 (TSP1). TSP1 expression by the epidermal keratinocytes is potently inhibited by UVB. It inhibits cutaneous angiogenesis and UVB-induced carcinogenesis. We show that apigenin restores TSP1 in epidermal keratinocytes subjected to UVB and normalizes proliferation and angiogenesis in UVB-exposed skin. Importantly, reconstituting TSP1 anti-angiogenic function in UVB-irradiated skin with a short bioactive peptide mimetic representing exclusively its anti-angiogenic domain reproduced the anti-proliferative and anti-angiogenic effects of apigenin. Cox-2 and HIF-1α are important mediators of angiogenesis. Both apigenin and TSP1 peptide mimetic attenuated their induction by UVB. Finally we identified the molecular mechanism, whereby apigenin did not affect TSP1 mRNA, but increased de novo protein synthesis. Knockdown studies implicated the RNA-binding protein HuR, which controls mRNA stability and translation. Apigenin increased HuR cytoplasmic localization and physical association with TSP1 mRNA causing de novo TSP1 synthesis. HuR cytoplasmic localization was, in turn, dependent on CHK2 kinase. Together, our data provide a new mechanism, by which apigenin controls UVB-induced carcinogenesis.

  3. Cannabinoid HU210 Protects Isolated Rat Stomach against Impairment Caused by Serum of Rats with Experimental Acute Pancreatitis

    Science.gov (United States)

    Cao, Ming-hua; Li, Yong-yu; Xu, Jing; Feng, Ya-jing; Lin, Xu-hong; Li, Kun; Han, Tong; Chen, Chang-Jie

    2012-01-01

    Acute pancreatitis (AP), especially severe acute pancreatitis often causes extra-pancreatic complications, such as acute gastrointestinal mucosal lesion (AGML) which is accompanied by a considerably high mortality, yet the pathogenesis of AP-induced AGML is still not fully understood. In this report, we investigated the alterations of serum components and gastric endocrine and exocrine functions in rats with experimental acute pancreatitis, and studied the possible contributions of these alterations in the pathogenesis of AGML. In addition, we explored the intervention effects of cannabinoid receptor agonist HU210 and antagonist AM251 on isolated and serum-perfused rat stomach. Our results showed that the AGML occurred after 5 h of AP replication, and the body homeostasis was disturbed in AP rat, with increased levels of pancreatic enzymes, lipopolysaccharide (LPS), proinflammtory cytokines and chemokines in the blood, and an imbalance of the gastric secretion function. Perfusing the isolated rat stomach with the AP rat serum caused morphological changes in the stomach, accompanied with a significant increment of pepsin and [H+] release, and increased gastrin and decreased somatostatin secretion. HU210 reversed the AP-serum-induced rat pathological alterations, including the reversal of transformation of the gastric morphology to certain degree. The results from this study prove that the inflammatory responses and the imbalance of the gastric secretion during the development of AP are responsible for the pathogenesis of AGML, and suggest the therapeutic potential of HU210 for AGML associated with acute pancreatitis. PMID:23285225

  4. Effect of route of infection on outcome of Toxoplasma gondii infection in hu-PBL SCID mice.

    Science.gov (United States)

    Meyer, Desiree J; Allan, Jane E; Beaman, Miles H

    2013-03-01

    Toxoplasma gondii (T. gondii) causes serious infection, especially in immunocompromised hosts. The relevance of animal models of toxoplasmosis to human disease is unclear, but have indicated that the route of Toxoplasma infection may affect the outcome. A humanized model of toxoplasmosis of immunocompromised mice (i.e. hu-PBL SCID), using the intraperitoneal (IP) route demonstrated long-term engraftment of human cells and worsening of inflammation compared to controls. In this study, we examined the effect of route of infection on this hu-PBL SCID model using a Toxoplasma strain (i.e. DAG) isolated from an immunocompromised human. Oral infection led to an asymptomatic infection, whereas animals infected by the IP route succumbed more quickly to infection. Human cells, detected through species-specific β-actin mRNA, were not as prominent in IP-infected animals as compared to orally infected and uninfected animals. There was evidence of control of toxoplasmosis in some orally infected animals, and this was associated with the presence of human cells in multiple tissues. Thus, the route of infection dramatically affects the outcome of infection, either by affecting parasite replication or expansion of human immune cells. Further studies of oral Toxoplasma infection using hu-PBL SCID mice may help in developing chemotherapies and immunotherapeutic strategies for toxoplasmosis.

  5. A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

    Directory of Open Access Journals (Sweden)

    Zhaoyang Zhang

    2011-11-01

    Full Text Available We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α. In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor, on the surface of which the complementary sequences are linked (as cODN-AuNPs and pre-hybridized with carboxymethylfluorescein (FAM-labeled anti-rHuEPO-α aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-α concentrations as low as 0.92 nM within a few hours.

  6. Comment on "Tropospheric temperature response to stratospheric ozone recovery in the 21st century" by Hu et al. (2011

    Directory of Open Access Journals (Sweden)

    M. Previdi

    2012-01-01

    Full Text Available Stratospheric ozone recovery is expected to figure prominently in twenty-first century climate change. In a recent paper, Hu et al. (2011 argue that one impact of ozone recovery will be to enhance the warming of the surface-troposphere system produced by increases in well-mixed greenhouse gases; furthermore, this enhanced warming would be strongest in the Northern Hemisphere, which is surprising since previous studies have consistently shown the effects of stratospheric ozone changes to be most pronounced in the Southern Hemisphere. Hu et al. (2011 base their claims largely on differences in the simulated temperature change between two groups of IPCC climate models, one group which included stratospheric ozone recovery in its twenty-first century simulations and a second group which did not. Both groups of models were forced with the same increases in well-mixed greenhouse gases according to the A1B emissions scenario. In the current work, we compare the surface temperature responses of the same two groups of models in a different experiment in which atmospheric CO2 was increased by 1% per year until doubling. We find remarkably similar differences in the simulated surface temperature change between the two sets of models as Hu et al. (2011 found for the A1B experiment, suggesting that the enhanced warming which they attribute to stratospheric ozone recovery is actually a reflection of different responses of the two model groups to greenhouse gas forcing.

  7. Morphological and kinematic signatures of a binary central star in the planetary nebula Hu 2-1

    CERN Document Server

    Miranda, L F; Guerrero, M; Vázquez, R A; Gómez, Y; Miranda, Luis F.; Torrelles, Jose M.; Guerrero, Martin A.; Vazquez, Roberto; Gomez, Yolanda

    2000-01-01

    We present narrow-band optical images, VLA-A 3.6 cm continuum and H92alpha emission line data and high resolution long-slit [N II] spectra of Hu 2-1. The formation of Hu 2-1 appears dominated by anisotropic mass ejection during the late AGB stage of its progenitor and by variable, ``precessing'' collimated bipolar outflows during the proto-PN and/or early PN phases. The data strongly support the existence of a binary central star at center of Hu 2-1: (1) the brightness distribution of the bipolar lobes and inner shell is point-symmetric, and the bipolar lobes show departures from axial symmetry; (2) the position of the central star is off-center; (3) mass ejection towards the equatorial plane is detected; (4) ``precessing'' collimated outflows are present. Besides all this circumstantial evidences, the analysis of the kinematics shows that the systemic velocity of the bipolar outflows does not coincide with the systemic velocity of the nebula. We propose that this velocity difference is a direct evidence of o...

  8. Modeling and predicting hemorrhagic fever with renal syndrome trends based on meteorological factors in Hu County, China.

    Directory of Open Access Journals (Sweden)

    Dan Xiao

    Full Text Available Hu County is a serious hemorrhagic fever with renal syndrome (HFRS epidemic area, with notable fluctuation of the HFRS epidemic in recent years. This study aimed to explore the optimal model for HFRS epidemic prediction in Hu.Three models were constructed and compared, including a generalized linear model (GLM, a generalized additive model (GAM, and a principal components regression model (PCRM. The fitting and predictive adjusted R2 of each model were calculated. Ljung-Box Q tests for fitted and predicted residuals of each model were conducted. The study period was stratified into before (1971-1993 and after (1994-2012 vaccine implementation epochs to avoid the confounding factor of vaccination.The autocorrelation of fitted and predicted residuals of the GAM in the two epochs were not significant (Ljung-Box Q test, P>.05. The adjusted R2 for the predictive abilities of the GLM, GAM, and PCRM were 0.752, 0.799, and 0.665 in the early epoch, and 0.669, 0.756, and 0.574 in the recent epoch. The adjusted R2 values of the three models were lower in the early epoch than in the recent epoch.GAM is superior to GLM and PCRM for monthly HFRS case number prediction in Hu County. A shift in model reliability coincident with vaccination implementation demonstrates the importance of vaccination in HFRS control and prevention.

  9. Orally administrated Juzen-taiho-to/TJ-48 ameliorates erythropoietin (rHuEPO)-resistant anemia in patients on hemodialysis.

    Science.gov (United States)

    Nakamoto, Hidetomo; Mimura, Taku; Honda, Nobuko

    2008-10-01

    Maintenance of the red blood cell volume is a fundamental aspect of ensuring oxygen supply to the tissue. Recombinant human erythropoietin (rHuEPO) was approved for marketing in Japan in 1990 for the treatment of anemia in patients on dialysis. Recombinant human erythropoietin caused a significant increase in hemoglobin (Hb) levels in patients on dialysis. However, not all have a good response to rHuEPO therapy; the causes of rHuEPO failure include iron deficiency, infection, uremia, and interaction of some drugs. Juzen-taiho-to (TJ-48), a mixture of extracts from 10 medicinal herbs, has been used traditionally to treat patients with anemia, anorexia, or fatigue. To clarify the effect of TJ-48 on erythropoietin-resistant anemia, we studied the effect of TJ-48 in patients on hemodialysis with erythropoietin-resistant anemia. We divided 42 end-stage renal disease patients on hemodialysis with erythropoietin-resistant anemia (Hbrenal disease patients. This effect was, at least in part, due to the anti-inflammatory effect of TJ-48 in patients on hemodialysis.

  10. The extraction and purification process of Qing Feng Teng in the Compound Qufeng sustained - release tablets%复方祛风缓释片中青风藤的提取纯化工艺研究

    Institute of Scientific and Technical Information of China (English)

    方达任; 张志

    2011-01-01

    目的 优选复方祛风缓释片中青风藤的提取纯化工艺.方法 以青藤碱作为指标,采用正交试验和单因素相结合的方法优选青风藤提取纯化工艺.结果 青藤碱最佳提取工艺为:70%的乙醇6倍量提取2次,每次2h;纯化工艺为:加1% HCl调pH值为2,氯仿萃取5次.结论 该提取纯化工艺科学、合理.%Objective Optimization of the extraction and purification process of Qing Feng Teng in the Compound Qufeng sustained -release tablets. Method With sinomenine as an indicator,orthogonal test and single factors combined to optimize of extraction and purification process of Qing Feng Teng. Result The best extraction of Sinomenine is; 70% ethanol 6 times capacity of Caulis Sinomenii, extracted 2 times, 2 hours per time;the purification technology are; using dilute HC1 to adjust PH to 2, then adding chloroform to extract 5 times. Conclusion The extraction and purification is scientific and rational.

  11. Clinical Study on Treatment of 85 Cases of Gouty Arthritis with Tong Feng Lotion%痛风洗剂治疗痛风性关节炎85例临床研究

    Institute of Scientific and Technical Information of China (English)

    张史昭; 马红珍; 李学铭

    2001-01-01

    150例患者分为两组,分别应用痛风洗剂(85例)及扶他林洗剂(65例)外用治疗,结果显示中药痛风洗剂对痛风患者具有改善临床症状及关节功能的作用,能降低血沉、C反应蛋白、血尿酸及血液粘度。治疗组总有效率为89.41%,与对照组(75.38%)比较差异有显著性(P<0.05)。无毒副作用,且治疗效果与中医证型无明显关系。%One hundred and fifty cases of gouty arthritis were divided into two groups.Treatment group(85 cases) were treated with Tong Feng lotion and control group(65 cases) with external application of Voltaren. Results indicated that Tong Feng lotion could improve clinical symptoms and function of joints, and decrease blood sedimentation,reaction protien C,blood uric acid and blood viscosity; the total effective rate was 89.42% in the treatment group and 75.38% in the control group, with significant difference between the two groups(P<0.05); there was no obvious relation between therapeutic effects and TCM syndrome types.

  12. 丰子恺音乐理论著述对我国近代音乐的影响%On the Influence of Feng Zikai’ s Writings of Music Theory on China’ s Modern Music

    Institute of Scientific and Technical Information of China (English)

    刘建东

    2015-01-01

    Feng Zikai’ s music writings cover various aspects including introductions to Western mu-sicians and their works, Western music history, music aesthetic education, music technological the-ories and musical aesthetics.Through the translation of Western music theories, Feng Zikai has made an outstanding contribution to the public understanding of the Western music in China, sinici-zation of Western music aesthetic theories as well as the specification and promotion of music educa-tion in schools, thus pushes the historical process of eastward flowing of the Western music as a whole.%丰子恺音乐著述的内容涉及西方音乐家及其作品介绍、西方音乐史、音乐审美教育、音乐技术理论、音乐美学等方面。丰子恺通过西方音乐理论的译介,对我国社会民众了解西方音乐、对西方音乐美学理论的中国化实践、对规范与提升学校音乐教育等方面均做出了突出的贡献,进而整体推动了我国社会“西乐东渐”的历史进程。

  13. A Research on Multiple Implications of Fomalhaut's Image in Feng Ge's Cang Hai%凤歌《沧海》"北落师门"形象的多重意蕴初探

    Institute of Scientific and Technical Information of China (English)

    刘中望

    2009-01-01

    On levels of text structure, image tune, referential meaning, Fomalhaut's animal image in Feng Ge's fiction Cang Hai loads multiple implications. It enhances the overall sense of the fiction, deepens mysterious color, generates deep meaning reference, conveys the global viewpoint on the relationship between Chinese and Western cultures, modernity, world image, etc, which reflects the unique features of Cang Hai and Feng Ge's continued innovation ability.%在文本结构、形象调性、意义指涉等层面,凤歌中"北落师门"动物形象具有多重意蕴:增强作品的一体感,凸显全书的玄幻性,生成深层的意义指涉,体现对中西文化关系、现代性、世界图景等的全球化思考,这反映了的独特魅力、凤歌的持续创新能力.

  14. Bone and muscle assessment in patients undergoing total hip arthroplasty using HU based analysis

    Directory of Open Access Journals (Sweden)

    Þröstur Pétursson

    2012-09-01

    Full Text Available Total hip arthroplasty (THA is performed with or without the use of bone cement. The lack of reliable clinical guidelines for deciding which one to implement has encouraged this approach of joint clinical and engineering with the following objectives: 1. Validate quadriceps muscles and femur bone atrophy by extracting the mineral density from Computer Tomographic (CT images. 2. Validate computational processes based on 3-D modeling and Finite Element Methods (FEM. A clinical trial was started, where 36 volunteer patients underwent THA surgery for the first time: 18 receiving cemented implant and 18 receiving uncemented implant. The patients were CT scanned prior-, immediately after and 12 months post-surgery. The CT data are further processed to segment muscles and bones and to create 3D-models for the simulation and for calculating bone mineral density (BMD. Furthermore quadriceps muscle density Hounsfield (HU based value is calculated from the segmented file on healthy and operated leg. These preliminary results indicate computational tools and methods that are able to quantitatively analyse patient’s condition pre and post-surgery. The BMD and muscle density measurement in correlation with the fracture risk analysis display a potential method for eligibility to receive non-cemented implant; the preliminary results show that also elderly that according with current clinical evaluation receives a cemented implant are suitable for the non-cemented type. The risk for structural failure during THA surgery is estimated by calculating femoral bone fracture risk index (FRI as a ratio between compressive stress during surgery and estimated failure stress on bone. The correlations with the BMD observations during the clinical trial will assess and validate this potential predictor tool.

  15. Publicidad y consumo de alimentos en estudiantes de Huánuco (Perú

    Directory of Open Access Journals (Sweden)

    María Luisa Sevillano García

    2012-10-01

    Full Text Available Las importantes carencias nutritivas apreciadas en estudiantes del distrito de Huánuco (Perú, junto con la publicidad televisiva de productos de dudosa calidad nutricional observada, nos llevó a plantear y desarrollar una investigación con el objeto de analizar la relación entre ambos fenómenos titulada: Influencia de los anuncios televisivos en el consumo de alimentos publicitados durante el 2010, cuyo resumen se presenta en este artículo. En la investigación planteamos un estudio correlacional con una muestra de 120 escolares de diversas instituciones educativas, a quienes se aplicaron satisfactoriamente encuestas, entrevistas y guía de evaluación física; también se analizó la publicidad emitida por los canales de televisión sobre alimentos y su contenido nutricional. El análisis inferencial aplicado fue Ji cuadrado (?2. Con esta investigación se ha demostrado que el estado de salud de los escolares está afectado, siendo muy agudo respecto a la caries dental, problema que se atribuye al consumo de ciertos alimentos. Se constata que existe relación positiva, con alta significancia estadística, entre la adherencia a los anuncios televisivos y el consumo de alimentos publicitados de alto contenido en sodio, calcio, azúcar y de calorías por los escolares estudiados; así mismo, se prueba la interdependencia entre la exposición a los anuncios televisivos y el consumo de alimentos con productos de alto contenido en grasa que merecen ser vigilados y controlados por los responsables.

  16. Benchmark critical experiments on low-enriched uranium oxide systems with H/U = 0. 77

    Energy Technology Data Exchange (ETDEWEB)

    Tuck, G; Oh, I

    1979-08-01

    Ten benchmark experiments were performed at the Critical Mass Laboratory at Rockwell International's Rocky Flats Plant, Golden, Colorado, for the US Nuclear Regulatory Commission. They provide accurate criticality data for low-enriched damp uranium oxide (U/sub 3/O/sub 8/) systems. The core studied consisted of 152 mm cubical aluminum cans containing an average of 15,129 g of low-enriched (4.46% /sup 235/U) uranium oxide compacted to a density of 4.68 g/cm/sup 3/ and with an H/U atomic ratio of 0.77. One hundred twenty five (125) of these cans were arranged in an approx. 770 mm cubical array. Since the oxide alone cannot be made critical in an array of this size, an enriched (approx. 93% /sup 235/U) metal or solution driver was used to achieve criticality. Measurements are reported for systems having the least practical reflection and for systems reflected by approx. 254-mm-thick concrete or plastic. Under the three reflection conditions, the mass of the uranium metal driver ranged from 29.87 kg to 33.54 kg for an oxide core of 1864.6 kg. For an oxide core of 1824.9 kg, the weight of the high concentration (351.2 kg U/m/sup 3/) solution driver varied from 14.07 kg to 16.14 kg, and the weight of the low concentration (86.4 kg U/m/sup 3/) solution driver from 12.4 kg to 14.0 kg.

  17. HuMiTar: A sequence-based method for prediction of human microRNA targets

    Directory of Open Access Journals (Sweden)

    Chen Ke

    2008-12-01

    Full Text Available Abstract Background MicroRNAs (miRs are small noncoding RNAs that bind to complementary/partially complementary sites in the 3' untranslated regions of target genes to regulate protein production of the target transcript and to induce mRNA degradation or mRNA cleavage. The ability to perform accurate, high-throughput identification of physiologically active miR targets would enable functional characterization of individual miRs. Current target prediction methods include traditional approaches that are based on specific base-pairing rules in the miR's seed region and implementation of cross-species conservation of the target site, and machine learning (ML methods that explore patterns that contrast true and false miR-mRNA duplexes. However, in the case of the traditional methods research shows that some seed region matches that are conserved are false positives and that some of the experimentally validated target sites are not conserved. Results We present HuMiTar, a computational method for identifying common targets of miRs, which is based on a scoring function that considers base-pairing for both seed and non-seed positions for human miR-mRNA duplexes. Our design shows that certain non-seed miR nucleotides, such as 14, 18, 13, 11, and 17, are characterized by a strong bias towards formation of Watson-Crick pairing. We contrasted HuMiTar with several representative competing methods on two sets of human miR targets and a set of ten glioblastoma oncogenes. Comparison with the two best performing traditional methods, PicTar and TargetScanS, and a representative ML method that considers the non-seed positions, NBmiRTar, shows that HuMiTar predictions include majority of the predictions of the other three methods. At the same time, the proposed method is also capable of finding more true positive targets as a trade-off for an increased number of predictions. Genome-wide predictions show that the proposed method is characterized by 1.99 signal

  18. miR-29b, miR-205 and miR-221 enhance chemosensitivity to gemcitabine in HuH28 human cholangiocarcinoma cells.

    Directory of Open Access Journals (Sweden)

    Kinya Okamoto

    Full Text Available BACKGROUND AND AIMS: Cholangiocarcinoma (CCA is highly resistant to chemotherapy, including gemcitabine (Gem treatment. MicroRNAs (miRNAs are endogenous, non-coding, short RNAs that can regulate multiple genes expression. Some miRNAs play important roles in the chemosensitivity of tumors. Here, we examined the relationship between miRNA expression and the sensitivity of CCA cells to Gem. METHODS: Microarray analysis was used to determine the miRNA expression profiles of two CCA cell lines, HuH28 and HuCCT1. To determine the effect of candidate miRNAs on Gem sensitivity, expression of each candidate miRNA was modified via either transfection of a miRNA mimic or transfection of an anti-oligonucleotide. Ontology-based programs were used to identify potential target genes of candidate miRNAs that were confirmed to affect the Gem sensitivity of CCA cells. RESULTS: HuCCT1 cells were more sensitive to Gem than were HuH28 cells, and 18 miRNAs were differentially expressed whose ratios over ± 2log2 between HuH28 and HuCCT1. Among these 18 miRNAs, ectopic overexpression of each of three downregulated miRNAs in HuH28 (miR-29b, miR-205, miR-221 restored Gem sensitivity to HuH28. Suppression of one upregulated miRNA in HuH28, miR-125a-5p, inhibited HuH28 cell proliferation independently to Gem treatment. Selective siRNA-mediated downregulation of either of two software-predicted targets, PIK3R1 (target of miR-29b and miR-221 or MMP-2 (target of miR-29b, also conferred Gem sensitivity to HuH28. CONCLUSIONS: miRNA expression profiling was used to identify key miRNAs that regulate Gem sensitivity in CCA cells, and software that predicts miRNA targets was used to identify promising target genes for anti-tumor therapies.

  19. MiR-291b-3p Induces Apoptosis in Liver Cell Line NCTC1469 by Reducing the Level of RNA-binding Protein HuR

    Directory of Open Access Journals (Sweden)

    Jun Guo

    2014-03-01

    Full Text Available Background: There is increasing evidence that miRNAs are involved in cellular apoptosis. However, the specific role of miR-291b-3p in apoptosis has not been elucidated. In the present study, we investigated the effect of miR-291b-3p on NCTC1469 cell growth and apoptosis. Methods: Cell viability and apoptosis were examined in NCTC1469 cells transfected with miR-291b-3p mimics, inhibitor miRNA or negative control. Using computational miRNA target prediction databases, HuR was predicted as a target of miR-291b-3p. Luciferase assay, immunofluorescence and western blot were used to further explore the effects of miR-291b-3p on HuR expression. In addition, the effect of HuR on cell apoptosis was evaluated using a HuR-specific siRNA. Results: TNF-α-induced hepatocyte apoptosis was accompanied by enhanced expression of miR-291b-3p, suggesting that miR-291b-3p might contribute to the apoptotic process. Follow-up experiments showed that upregulation of miR-291b-3p decreased cell viability and induced NCTC1469 cell apoptosis. Additionally, similar to the activity of miR-519, which is another member of the same miRNA family, miR-291b-3p suppressed HuR translation through binding to the HuR coding region (CR. We further showed that the downregulation of HuR expression by miR-291b-3p was accompanied by reduced Bcl-2 expression. Moreover, knockdown of HuR also impaired Bcl-2 expression and increased the ratio of Bax/Bcl-2. More significantly, downregulation of miR-291b-3p failed to increase Bcl2 expression in NCTC1469 cells that were co-transfected with siRNA-HuR. Finally, inhibition of miR-291b-3p led to reduced apoptosis, while knockdown of HuR by siRNA promoted apoptosis, even in NCTC1469 cells that were co-transfected with the miR-291b-3p inhibitor. Conclusion: The current data suggested that miR-291b-3p contributed to NCTC1469 cell apoptosis by regulating the expression of HuR, which in turn increased Bcl-2 stability.

  20. PKCε promotes HuD-mediated neprilysin mRNA stability and enhances neprilysin-induced Aβ degradation in brain neurons.

    Directory of Open Access Journals (Sweden)

    Chol Seung Lim

    Full Text Available Amyloid-beta (Aβ peptide accumulation in the brain is a pathological hallmark of all forms of Alzheimer's disease. An imbalance between Aβ production and clearance from the brain may contribute to accumulation of neurotoxic Aβ and subsequent synaptic loss, which is the strongest correlate of the extent of memory loss in AD. The activity of neprilysin (NEP, a potent Aβ-degrading enzyme, is decreased in the AD brain. Expression of HuD, an mRNA-binding protein important for synaptogenesis and neuronal plasticity, is also decreased in the AD brain. HuD is regulated by protein kinase Cε (PKCε, and we previously demonstrated that PKCε activation decreases Aβ levels. We hypothesized that PKCε acts through HuD to stabilize NEP mRNA, modulate its localization, and support NEP activity. Conversely, loss of PKCε-activated HuD in AD leads to decreased NEP activity and accumulation of Aβ. Here we show that HuD is associated with NEP mRNA in cultures of human SK-N-SH cells. Treatment with bryostatin, a PKCε-selective activator, enhanced NEP association with HuD and increased NEP mRNA stability. Activation of PKCε also increased NEP protein levels, increased NEP phosphorylation, and induced cell surface expression. In addition, specific PKCε activation directly stimulated NEP activity, leading to degradation of a monomeric form of Aβ peptide and decreased Aβ neuronal toxicity, as measured by cell viability. Bryostatin treatment also rescued Aβ-mediated inhibition of HuD-NEP mRNA binding, NEP protein expression, and NEP cell membrane translocation. These results suggest that PKCε activation reduces Aβ by up-regulating, via the mRNA-binding protein HuD, Aβ-degrading enzymes such as NEP. Thus, PKCε activation may have therapeutic efficacy for AD by reducing neurotoxic Aβ accumulation as well as having direct anti-apoptotic and synaptogenic effects.

  1. President Hu is in India but Who Am I in China?%胡主席在印度,我却在中国

    Institute of Scientific and Technical Information of China (English)

    Rajesh Kanoi (Jack)

    2007-01-01

    @@ Today, Sunday morning, I sit thinking and typing at my computer. My thoughts are in my homeland, India and my body is in China. The body is here and mind there for a good reason. Mr. Hu is president and I am resident and both are in each other's countries (what a difference the absence of one small letter makes!). Mr. Hu will return in a few days and I will stay on longer.

  2. HuGE Watch: tracking trends and patterns of published studies of genetic association and human genome epidemiology in near-real time.

    Science.gov (United States)

    Yu, Wei; Wulf, Anja; Yesupriya, Ajay; Clyne, Melinda; Khoury, Muin Joseph; Gwinn, Marta

    2008-09-01

    HuGE Watch is a web-based application for tracking the evolution of published studies on genetic association and human genome epidemiology in near-real time. The application allows users to display temporal trends and spatial distributions as line charts and google maps, providing a quick overview of progress in the field. http://www.hugenavigator.net/HuGENavigator/startPageWatch.do

  3. RNA-binding protein HuR sequesters microRNA-21 to prevent translation repression of proinflammatory tumor suppressor gene programmed cell death 4.

    Science.gov (United States)

    Poria, D K; Guha, A; Nandi, I; Ray, P S

    2016-03-31

    Translation control of proinflammatory genes has a crucial role in regulating the inflammatory response and preventing chronic inflammation, including a transition to cancer. The proinflammatory tumor suppressor protein programmed cell death 4 (PDCD4) is important for maintaining the balance between inflammation and tumorigenesis. PDCD4 messenger RNA translation is inhibited by the oncogenic microRNA, miR-21. AU-rich element-binding protein HuR was found to interact with the PDCD4 3'-untranslated region (UTR) and prevent miR-21-mediated repression of PDCD4 translation. Cells stably expressing miR-21 showed higher proliferation and reduced apoptosis, which was reversed by HuR expression. Inflammatory stimulus caused nuclear-cytoplasmic relocalization of HuR, reversing the translation repression of PDCD4. Unprecedentedly, HuR was also found to bind to miR-21 directly, preventing its interaction with the PDCD4 3'-UTR, thereby preventing the translation repression of PDCD4. This suggests that HuR might act as a 'miRNA sponge' to regulate miRNA-mediated translation regulation under conditions of stress-induced nuclear-cytoplasmic translocation of HuR, which would allow fine-tuned gene expression in complex regulatory environments.

  4. Changes in Cellular mRNA Stability, Splicing, and Polyadenylation through HuR Protein Sequestration by a Cytoplasmic RNA Virus

    Directory of Open Access Journals (Sweden)

    Michael D. Barnhart

    2013-11-01

    Full Text Available The impact of RNA viruses on the posttranscriptional regulation of cellular gene expression is unclear. Sindbis virus causes a dramatic relocalization of the cellular HuR protein from the nucleus to the cytoplasm in infected cells. This is to the result of the expression of large amounts of viral RNAs that contain high-affinity HuR binding sites in their 3′ UTRs effectively serving as a sponge for the HuR protein. Sequestration of HuR by Sindbis virus is associated with destabilization of cellular mRNAs that normally bind HuR and rely on it to regulate their expression. Furthermore, significant changes can be observed in nuclear alternative polyadenylation and splicing events on cellular pre-mRNAs as a result of sequestration of HuR protein by the 3′ UTR of transcripts of this cytoplasmic RNA virus. These studies suggest a molecular mechanism of virus-host interaction that probably has a significant impact on virus replication, cytopathology, and pathogenesis.

  5. UVB-dependent changes in the expression of fast-responding early genes is modulated by huCOP1 in keratinocytes.

    Science.gov (United States)

    Fazekas, B; Polyánka, H; Bebes, A; Tax, G; Szabó, K; Farkas, K; Kinyó, A; Nagy, F; Kemény, L; Széll, M; Ádám, É

    2014-11-01

    Ultraviolet (UV) B is the most prominent physical carcinogen in the environment leading to the development of various skin cancers. We have previously demonstrated that the human ortholog of the Arabidopsis thaliana constitutive photomorphogenesis 1 (COP1) protein, huCOP1, is expressed in keratinocytes in a UVB-regulated manner and is a negative regulator of p53 as a posttranslational modifier. However, it was not known whether huCOP1 plays a role in mediating the UVB-induced early transcriptional responses of human keratinocytes. In this study, we report that stable siRNA-mediated silencing of huCOP1 affects the UVB response of several genes within 2 h of irradiation, indicating that altered huCOP1 expression sensitizes the cells toward UVB. Pathway analysis identified a molecular network in which 13 of the 30 examined UVB-regulated genes were organized around three central proteins. Since the expression of the investigated genes was upregulated by UVB in the siCOP1 cell line, we hypothesize that huCOP1 is a repressor of the identified pathway. Several members of the network have been implicated previously in the pathogenesis of non-melanoma skin cancers; therefore, clarifying the role of huCOP1 in these skin diseases may have clinical relevance in the future.

  6. Effect of HU308 on microglia induced by LPS in secretion of NO and IL-6%HU308对脂多糖诱导小胶质细胞分泌NO及IL-6的影响

    Institute of Scientific and Technical Information of China (English)

    赵建云; 邓展进; 刘瑞珍

    2014-01-01

    Objective To study the effect in secretion of NO and IL-6 of cannabinoid CB2 receptor agonist (HU308) on microglia induced by lipopolysaccharide (LPS). Methods vitro culture BV-2 microglia were divided into control group, LPS stimulation group and intervention group (LPS + HU308). The morphological changes in microglia were observed by microscope, the proliferation in microglia was detected by CCK-8, the NO contents were detected by Griess reaction, the IL-6 contents were detected by ELISA. Results A large number of microglia in LPS stimulation group showed auxetic body, stubby or disappeared pseudopodium and necrotic cells, cell proliferation was poor, the expression level of NO and IL-6 increased significantly. After the intervention of CB2 receptor agonist (HU308), most of microglia showed slightly large body, obvious pseudopodia and light damage, cell proliferation was better, the expression of inflammatory factor decreased significantly. Conclusion Activating CB2 receptors expressing in microglia can alleviate the excessive activation of microglia caused by LPS, inhibit the secretion of N0 and IL-6, so as to achieve the protective effect of central nervous system inflammatory injury.%目的:研究大麻素CB2受体激动剂HU308对脂多糖(LPS)诱导小胶质细胞激活后NO及IL-6分泌的影响。方法体外培养小鼠小胶质细胞株(BV-2细胞),分为对照组、LPS刺激组及干预组(LPS+HU308)。通过显微镜观察各组小胶质细胞的形态学变化,CCK-8法检测各组小胶质细胞的增殖情况,Griess法检测各组NO含量,ELISA法检测各组IL-6水平。结果LPS刺激组的小胶质细胞中出现大量胞体增大,伪足粗短或消失的细胞和一些坏死细胞,细胞增殖较差,NO及IL-6表达水平显著增高。经大麻素CB2受体激动剂HU308干预后,大部分细胞胞体稍大,伪足尚明显,细胞破坏程度轻,增殖较好,炎性因子表达均明显下降。结论激动小

  7. Purification and characterization of a novel chlorpyrifos hydrolase from Cladosporium cladosporioides Hu-01.

    Science.gov (United States)

    Gao, Yan; Chen, Shaohua; Hu, Meiying; Hu, Qiongbo; Luo, Jianjun; Li, Yanan

    2012-01-01

    Chlorpyrifos is of great environmental concern due to its widespread use in the past several decades and its potential toxic effects on human health. Thus, the degradation study of chlorpyrifos has become increasing important in recent years. A fungus capable of using chlorpyrifos as the sole carbon source was isolated from organophosphate-contaminated soil and characterized as Cladosporium cladosporioides Hu-01 (collection number: CCTCC M 20711). A novel chlorpyrifos hydrolase from cell extract was purified 35.6-fold to apparent homogeneity with 38.5% overall recovery by ammoniumsulfate precipitation, gel filtration chromatography and anion-exchange chromatography. It is a monomeric structure with a molecular mass of 38.3 kDa. The pI value was estimated to be 5.2. The optimal pH and temperature of the purified enzyme were 6.5 and 40°C, respectively. No cofactors were required for the chlorpyrifos-hydrolysis activity. The enzyme was strongly inhibited by Hg²⁺, Fe³⁺, DTT, β-mercaptoethanol and SDS, whereas slight inhibitory effects (5-10% inhibition) were observed in the presence of Mn²⁺, Zn²⁺, Cu²⁺, Mg²⁺, and EDTA. The purified enzyme hydrolyzed various organophosphorus insecticides with P-O and P-S bond. Chlorpyrifos was the preferred substrate. The Km and Vmax values of the enzyme for chlorpyrifos were 6.7974 μM and 2.6473 μmol·min⁻¹, respectively. Both NH2-terminal sequencing and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometer (MALDI-TOF-MS) identified an amino acid sequence MEPDGELSALTQGANS, which shared no similarity with any reported organophosphate-hydrolyzing enzymes. These results suggested that the purified enzyme was a novel hydrolase and might conceivably be developed to fulfill the practical requirements to enable its use in situ for detoxification of chlorpyrifos. Finally, this is the first described chlorpyrifos hydrolase from fungus.

  8. 丰子恺的佛学价值取向初探%Feng Zikai’s Orientation of Budhist Value

    Institute of Scientific and Technical Information of China (English)

    冉祥华

    2015-01-01

    丰子恺推重佛学,是受弘一法师的影响,但是他的佛学思想比较驳杂,华严宗、法相宗、净土宗、禅宗、律宗等在其身上都有所体现。丰子恺信佛但不迷信佛,不是为了成佛,更不是为了获得与佛同在的最高幸福。他信佛的目的只有一个,那就是“以精神生活代替物质生活”。在他的眼里,佛只是一种最高的人格境界。佛教的“无我说”乃苦难的解脱之道。“无我”首先要抛弃“我私我欲”的贪念,正是在这一基点上,丰子恺毕生笃守佛家信念,并身体力行致力于个人品性的修炼。丰子恺对佛教中的迷信部分始终是否定的,他强调直接用心灵与佛陀的精神进行“对话”,去理解佛陀物我一体的慈悲心和四大皆空的基本义,这无疑与“五四”时代对待宗教的态度是一致的。%Influenced by Master Hong Yi,Feng Zikai has high regard for Buddhism,but his Buddhist thoughts are more complex,which embodied Huayan School,Hossoshu,Pure-land School,Zen,Vinaya School,and so on.Though he trusts in Buddhism,he has neither blind faith on it nor becomes a Buddha,even nor obtains the supreme happiness of being with Buddha.It is “to substitute the spiritual life for the material life”that is his only purpose.Buddhism,in which “the Theory of Anatman”is the way to relieve oneself from suffering,is only the supreme realm of personality in his mind.Being “anatman”,one must firstly abandon “oneself and his desires”,in which he keeps his belief all his life and cultivates his personality with his practice.He keeps a negative attitude to the superstition belief in Buddhism,and emphasizes the direct dialogue between one’s soul and the spirit of Buddha to understand Buddha’s compassion of “the integrity of oneself and the universe”and the essential meaning of “being void of vanities”,which is undoubtedly consistent with the attitude towards religions

  9. A Bite of Hu Flavour in Diets of Tang Dynasty%唐代舌尖上的胡味

    Institute of Scientific and Technical Information of China (English)

    刘锡涛

    2015-01-01

    Tang Dynasty was an innovative, open and tolerant society with booming economy and constant re-newals, which was characterized by strong Hu flavor in diet. There were plenty kinds of Hu diets on staples, vegetables, fruits, wines and flavourings. While pastries were the staple diet, there were such vegetables as spinach, cucumber, eggplant, preserved pickle, lettuce and such fruits as golden peach, grape, date and fla-vourings as sugar, parsley, pepper and such wine as grape wine, long-gao wine. The popularity of Hu diet in Tang Dynasty accelerates the multi-ethnic mélange of dietetic cultures.%唐代是个改革、开放与包容的社会,经济发展,吐旧纳新,人们在饮食上多体现出胡味现象,胡食品种繁多,集中体现在主食、蔬菜、水果、酒及调味品上。主食多吃面饼,蔬菜主要有菠菜、黄瓜、茄子、榨菜、莴苣等,水果有金桃、葡萄、波斯枣等,调味品主要有蔗糖、香菜、胡椒等,酒有葡萄酒、龙膏酒等水果酒。唐代胡食的盛行,加快了我国多民族饮食文化的融合发展。

  10. Les structures génotypiques multilocus des populations d'huîtres plates en France

    Directory of Open Access Journals (Sweden)

    Françoise BLANC

    1987-09-01

    Full Text Available La structure génétique des populations de l'huître plate Ostrea edulis est décrite sur la base des génotypes multilocus. Associée au traitement des données de type multivarié, cette nouvelle procédure permet d'identifier les sous-ensembles génétiques intrapopulationnels qui peuvent être repérés dans un échantillon composite résultant de transferts comme dans la lagune de Thau.

  11. pp32 (ANP32A expression inhibits pancreatic cancer cell growth and induces gemcitabine resistance by disrupting HuR binding to mRNAs.

    Directory of Open Access Journals (Sweden)

    Timothy K Williams

    Full Text Available The expression of protein phosphatase 32 (PP32, ANP32A is low in poorly differentiated pancreatic cancers and is linked to the levels of HuR (ELAV1, a predictive marker for gemcitabine response. In pancreatic cancer cells, exogenous overexpression of pp32 inhibited cell growth, supporting its long-recognized role as a tumor suppressor in pancreatic cancer. In chemotherapeutic sensitivity screening assays, cells overexpressing pp32 were selectively resistant to the nucleoside analogs gemcitabine and cytarabine (ARA-C, but were sensitized to 5-fluorouracil; conversely, silencing pp32 in pancreatic cancer cells enhanced gemcitabine sensitivity. The cytoplasmic levels of pp32 increased after cancer cells are treated with certain stressors, including gemcitabine. pp32 overexpression reduced the association of HuR with the mRNA encoding the gemcitabine-metabolizing enzyme deoxycytidine kinase (dCK, causing a significant reduction in dCK protein levels. Similarly, ectopic pp32 expression caused a reduction in HuR binding of mRNAs encoding tumor-promoting proteins (e.g., VEGF and HuR, while silencing pp32 dramatically enhanced the binding of these mRNA targets. Low pp32 nuclear expression correlated with high-grade tumors and the presence of lymph node metastasis, as compared to patients' tumors with high nuclear pp32 expression. Although pp32 expression levels did not enhance the predictive power of cytoplasmic HuR status, nuclear pp32 levels and cytoplasmic HuR levels associated significantly in patient samples. Thus, we provide novel evidence that the tumor suppressor function of pp32 can be attributed to its ability to disrupt HuR binding to target mRNAs encoding key proteins for cancer cell survival and drug efficacy.

  12. Saturated fatty acids induce post-transcriptional regulation of HAMP mRNA via AU-rich element-binding protein, human antigen R (HuR).

    Science.gov (United States)

    Lu, Sizhao; Mott, Justin L; Harrison-Findik, Duygu Dee

    2015-10-02

    Iron is implicated in fatty liver disease pathogenesis. The human hepcidin gene, HAMP, is the master switch of iron metabolism. The aim of this study is to investigate the regulation of HAMP expression by fatty acids in HepG2 cells. For these studies, both saturated fatty acids (palmitic acid (PA) and stearic acid (SA)) and unsaturated fatty acid (oleic acid (OA)) were used. PA and, to a lesser extent, SA, but not OA, up-regulated HAMP mRNA levels, as determined by real-time PCR. To understand whether PA regulates HAMP mRNA at the transcriptional or post-transcriptional level, the transcription inhibitor actinomycin D was employed. PA-mediated induction of HAMP mRNA expression was not blocked by actinomycin D. Furthermore, PA activated HAMP 3'-UTR, but not promoter, activity, as shown by reporter assays. HAMP 3'-UTR harbors a single AU-rich element (ARE). Mutation of this ARE abolished the effect of PA, suggesting the involvement of ARE-binding proteins. The ARE-binding protein human antigen R (HuR) stabilizes mRNA through direct interaction with AREs on 3'-UTR. HuR is regulated by phosphorylation-mediated nucleo-cytoplasmic shuttling. PA activated this process. The binding of HuR to HAMP mRNA was also induced by PA in HepG2 cells. Silencing of HuR by siRNA abolished PA-mediated up-regulation of HAMP mRNA levels. PKC is known to phosphorylate HuR. Staurosporine, a broad-spectrum PKC inhibitor, inhibited both PA-mediated translocation of HuR and induction of HAMP expression. Similarly, rottlerin, a novel class PKC inhibitor, abrogated PA-mediated up-regulation of HAMP expression. In conclusion, lipids mediate post-transcriptional regulation of HAMP throughPKC- and HuR-dependent mechanisms.

  13. HuR and podoplanin expression is associated with a high risk of malignant transformation in patients with oral preneoplastic lesions

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Higashino, Fumihiro; Hida, Kyoko; Totsuka, Yasunori; Shindoh, Masanobu

    2016-01-01

    The risk of malignant transformation in oral preneoplastic lesions (OPLs) is challenging to assess. The objective of the present study was to determine the expression of ELAV like RNA binding protein 1 (HuR) and podoplanin in OPLs, and to evaluate the use of each protein as biomarkers for the risk assessment of malignant transformations. Immunohistochemistry for HuR and podoplanin was performed on the tissues of 51 patients with OPL, including cases of low grade dysplasia (LGD) and high grade dysplasia (HGD). The association between the protein expression patterns and clinicopathological parameters, including oral cancer free survival (OCFS) time, was analyzed during the follow-up period. HuR and podoplanin expression was observed in 28 (55%) and 36 (71%) of 51 patients, respectively. Kaplan-Meier analysis showed that the expression of HuR and podoplanin was associated with the risk of progression to oral cancer (P<0.05). Multivariate analysis revealed that HuR and podoplanin expression was associated with a 2.93-fold (95% confidence interval (CI), 0.98–10.34; P=0.055) and 2.06-fold (95% CI, 0.55–8.01; P=0.283) increase in risk of malignant transformation, respectively. The risk of OPL malignant transformation was considerably increased with the coexpression of HuR and podoplanin compared with the histological grading (95% CI, 1.64–23.59; P=0.005). The results of the present study demonstrated that the expression of HuR and podoplanin associates with malignant transformation and suggests that the proteins may be used as biomarkers to identify OPL patients with an increased risk of cancer development. PMID:27899983

  14. GC–MS-Based Metabonomic Profiling Displayed Differing Effects of Borna Disease Virus Natural Strain Hu-H1 and Laboratory Strain V Infection in Rat Cortical Neurons

    Directory of Open Access Journals (Sweden)

    Siwen Liu

    2015-08-01

    Full Text Available Borna disease virus (BDV persists in the central nervous systems of a wide variety of vertebrates and causes behavioral disorders. Previous studies have revealed that metabolic perturbations are associated with BDV infection. However, the pathophysiological effects of different viral strains remain largely unknown. Rat cortical neurons infected with human strain BDV Hu-H1, laboratory BDV Strain V, and non-infected control (CON cells were cultured in vitro. At day 12 post-infection, a gas chromatography coupled with mass spectrometry (GC–MS metabonomic approach was used to differentiate the metabonomic profiles of 35 independent intracellular samples from Hu-H1-infected cells (n = 12, Strain V-infected cells (n = 12, and CON cells (n = 11. Partial least squares discriminant analysis (PLS-DA was performed to demonstrate discrimination between the three groups. Further statistical testing determined which individual metabolites displayed significant differences between groups. PLS-DA demonstrated that the whole metabolic pattern enabled statistical discrimination between groups. We identified 31 differential metabolites in the Hu-H1 and CON groups (21 decreased and 10 increased in Hu-H1 relative to CON, 35 differential metabolites in the Strain V and CON groups (30 decreased and 5 increased in Strain V relative to CON, and 21 differential metabolites in the Hu-H1 and Strain V groups (8 decreased and 13 increased in Hu-H1 relative to Strain V. Comparative metabonomic profiling revealed divergent perturbations in key energy and amino acid metabolites between natural strain Hu-H1 and laboratory Strain V of BDV. The two BDV strains differentially alter metabolic pathways of rat cortical neurons in vitro. Their systematic classification provides a valuable template for improved BDV strain definition in future studies.

  15. Increase of the RNA-binding protein HuD and posttranscriptional up-regulation of the GAP-43 gene during spatial memory

    Science.gov (United States)

    Pascale, Alessia; Gusev, Pavel A.; Amadio, Marialaura; Dottorini, Tania; Govoni, Stefano; Alkon, Daniel L.; Quattrone, Alessandro

    2004-01-01

    Neuronal ELAV-like proteins (HuB, HuC, and HuD) are highly conserved RNA-binding proteins able to selectively associate with the 3′ UTR of a subset of target mRNAs and increase their cytoplasmic stability and rate of translation. We previously demonstrated the involvement of these proteins in learning, reporting that they undergo a sustained up-regulation in the hippocampus of mice trained in a spatial discrimination task. Here, we extend this finding, showing that a similar up-regulation occurs in the hippocampus of rats trained in another spatial learning paradigm, the Morris water maze. HuD, a strictly neuron-specific ELAV-like protein, is shown to increase after learning, with a preferential binding to the cytoskeletal fraction. HuD up-regulation is associated with an enhancement of GAP-43 mRNA and protein levels, with an apparently increased HuD colocalization with the GAP-43 mRNA and an increased association of neuronal ELAV-like proteins with the GAP-43 mRNA. These learning-dependent biochemical events appear to be spatiotemporally controlled, because they do not occur in another brain region involved in learning, the retrosplenial cortex, and at the level of protein expression they show extinction 1 month after training despite memory retention. By contrast, HuD mRNA levels still remain increased after 1 month in the CA1 region. This persistence may have implications for long-term memory recall. PMID:14745023

  16. HuR and podoplanin expression is associated with a high risk of malignant transformation in patients with oral preneoplastic lesions.

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Higashino, Fumihiro; Hida, Kyoko; Totsuka, Yasunori; Shindoh, Masanobu

    2016-11-01

    The risk of malignant transformation in oral preneoplastic lesions (OPLs) is challenging to assess. The objective of the present study was to determine the expression of ELAV like RNA binding protein 1 (HuR) and podoplanin in OPLs, and to evaluate the use of each protein as biomarkers for the risk assessment of malignant transformations. Immunohistochemistry for HuR and podoplanin was performed on the tissues of 51 patients with OPL, including cases of low grade dysplasia (LGD) and high grade dysplasia (HGD). The association between the protein expression patterns and clinicopathological parameters, including oral cancer free survival (OCFS) time, was analyzed during the follow-up period. HuR and podoplanin expression was observed in 28 (55%) and 36 (71%) of 51 patients, respectively. Kaplan-Meier analysis showed that the expression of HuR and podoplanin was associated with the risk of progression to oral cancer (P<0.05). Multivariate analysis revealed that HuR and podoplanin expression was associated with a 2.93-fold (95% confidence interval (CI), 0.98-10.34; P=0.055) and 2.06-fold (95% CI, 0.55-8.01; P=0.283) increase in risk of malignant transformation, respectively. The risk of OPL malignant transformation was considerably increased with the coexpression of HuR and podoplanin compared with the histological grading (95% CI, 1.64-23.59; P=0.005). The results of the present study demonstrated that the expression of HuR and podoplanin associates with malignant transformation and suggests that the proteins may be used as biomarkers to identify OPL patients with an increased risk of cancer development.

  17. Construction of Eukaryotic Expression Vector Containing HuCTLA4Ig and its Expression in Mammalian Cells%HuCTLA4Ig真核表达载体的构建及其表达

    Institute of Scientific and Technical Information of China (English)

    祁小平; 钱军华; 谢晋; 马宝骊

    2000-01-01

    用RT-PCR方法从人外周血单个核细胞总RNA中分别扩增出CTLA4的胞膜外区基因和Igγ1恒定区基因,将它们拼接后插入质粒pcDNA3.将重组的HuCTLA4Ig/pcDNA质粒转染细胞,经筛选得到了高效、稳定表达HuCTLA4Ig的克隆.培养上清经ProteinA亲和层析柱纯化后,所得蛋白在非还原条件下SDS-PAGE呈分子量为11kD的一条带;FACS检测显示该纯化蛋白与B7分子有良好的结合能力;在体外能抑制MLR的增殖反应.

  18. 下梅古村落风水意象及养生内涵的解读%Mei Feng Shui Ancient Villages under the Imagery and Health Connotation Interpretation

    Institute of Scientific and Technical Information of China (English)

    周世辉

    2015-01-01

    This article through the analysis of carding mei feng shui cultural resources of ancient villages under the living experience, analyzes in cas fukang "content" under the guidance of the concept, more than five hundred to more than sixty kinds of surname how an ancient village of three thousand generations live in this is subject to ex-plore and crack in this paper.%笔者通过分析梳理下梅古村落风水人文资源中的养生经验, 剖析在"物阜民安"理念指导下,500多户60多种姓氏近3 000人口的古村落如何世代在此安居乐业,并对此逐一进行探究和解读.

  19. 冯金福教授半导体电子输运研究工作评述%A Review of Professor Feng Jin-Fu’s Research on the Electronic Transport Properties of Semiconductor

    Institute of Scientific and Technical Information of China (English)

    刘玉申

    2014-01-01

    This paper conducts a review of Professor Feng Jin-fu’s research on the field of the electronic trans-port properties of semiconductor from four aspects: spatial modulation differential reflectance spectroscopy, quan-tum theory of mesoscopic electrical circuit, quantum transport properties of quantum dot and DNA and spin char-acteristic of quantum dot.%从空间调制微分反射光谱、介观电路的量子力学处理、量子点和DNA的量子输运性质、量子点的自旋特性四个方面介绍了冯金福教授开展的在半导体电子输运领域所做的工作。

  20. HuR cytoplasmic expression is associated with increased cyclin A expression and poor outcome with upper urinary tract urothelial carcinoma

    Directory of Open Access Journals (Sweden)

    Liang Peir-In

    2012-12-01

    Full Text Available Abstract Background HuR is an RNA-binding protein that post-transcriptionally modulates the expressions of various target genes implicated in carcinogenesis, such as CCNA2 encoding cyclin A. No prior study attempted to evaluate the significance of HuR expression in a large cohort with upper urinary tract urothelial carcinomas (UTUCs. Methods In total, 340 cases of primary localized UTUC without previous or concordant bladder carcinoma were selected. All of these patients received ureterectomy or radical nephroureterectomy with curative intents. Pathological slides were reviewed, and clinical findings were collected. Immunostaining for HuR and cyclin A was performed and evaluated by using H-score. The results of cytoplasmic HuR and nuclear cyclin A expressions were correlated with disease-specific survival (DSS, metastasis-free survival (MeFS, urinary bladder recurrence-free survival (UBRFS, and various clinicopathological factors. Results HuR cytoplasmic expression was significantly related to the pT status, lymph node metastasis, a higher histological grade, the pattern of invasion, vascular and perineurial invasion, and cyclin A expression (p = 0.005. Importantly, HuR cytoplasmic expression was strongly associated with a worse DSS (p p p = 0.0370 in the univariate analysis, and the first two results remained independently predictive of adverse outcomes (p = 0.038, relative risk [RR] = 1.996 for DSS; p = 0.027, RR = 1.880 for MeFS. Cyclin A nuclear expression was associated with a poor DSS (p = 0.0035 and MeFS (p = 0.0015 in the univariate analysis but was not prognosticatory in the multivariate analyses. High-risk patients (pT3 or pT4 with/without nodal metastasis with high HuR cytoplasmic expression had better DSS if adjuvant chemotherapy was performed (p = 0.015. Conclusions HuR cytoplasmic expression was correlated with adverse phenotypes and cyclin A overexpression and also independently predictive

  1. 论冯契广义认识论中的两条认识进路%On the two Approaches of Cognition in Feng Qi's General Epistemology

    Institute of Scientific and Technical Information of China (English)

    章伟

    2012-01-01

    传统马克思主义哲学教材无外是沿着"感性经验—理论思维—具体真理"进路对认识论进行阐述。这一认识进路克服了先验论和经验论的缺陷,但也存在诸多弊端;特别是其淡化了主体自由人格的发展。而在冯契的广义认识论中,除传统认识进路以外,还突显出"认知—评价—理想"进路。它对前者的不足有所克服。比较这两条认识进路,可以进一步挖掘冯契对认识论的独特贡献。%The traditional materials of Marxist philosophy demonstrated the epistemology only along the approach of 'perceptual experience-theoretic thinking-specific truth'.This approach of cognition has overcome the defects of apriorism and empiricism,but still some shortcomings in it;it has neglected especially the development of the free personality.On the contrary,it is in Feng Qi's General Epistemology that an other approach is prominent besides the traditional one,named'cognition-valuation-ideal',which resolves the troubles of the former to some extent.We can go further into the special contribution of the epistemology from Feng Qi by contrasting them.

  2. ZhengQing FengTongNing injection iontophoresis treatment of rheumatoid arthritis%正清风痛宁注射液离子导入治疗风湿性关节炎

    Institute of Scientific and Technical Information of China (English)

    王娅娟

    2013-01-01

      目的 临床应用正清风痛宁注射液离子导入法治疗风湿性关节炎,观察其临床疗效及进行安全评估.方法将60例临床诊断风湿性关节炎患者随机双盲分为治疗组与对照组,每组各30例;对照组予细胞毒药物+糖皮质激素+非甾体类抗炎药联合治疗,治疗组在对照组基础上加用正清风痛宁注射液离子导入治疗,治疗1个月后,观察两组治疗疗效及不良反应.结果治疗组在临床疗效上优于对照组(P 0.05).结论正清风痛宁注射液离子导入治疗风湿性关节炎具有临床实用意义.%Objective ZhengQing FengTongNing injection iontophoresis treatment of rheumatoid arthritis. Methods 60 patients with clinical diagnosis of rheumatoid arthritis patients were randomly divided into treatment group and control group 30 cases each,after 1 month of therapy group to observe the curative effect and adverse reaction.Results he treatment group was better than control group in the clinical curative effect(P < 0.05). Conclusion ZhengQing FengTongNing injection iontophoresis treatment of rheumatoid arthritis is safe and effective.

  3. A new human cholangiocellular carcinoma cell line (HuCC-T1) producing carbohydrate antigen 19/9 in serum-free medium.

    Science.gov (United States)

    Miyagiwa, M; Ichida, T; Tokiwa, T; Sato, J; Sasaki, H

    1989-06-01

    A human cholangiocellular carcinoma cell line, HuCC-T1, was established in vitro from the malignant cells of ascites of a 56-yr-old patient. Histologic findings of the primary liver tumor revealed a moderately differentiated adenocarcinoma. Tumor cells from the ascites have been cultured with RPMI 1640 medium containing 0.2% lactalbumin hydrolysate and the cultured cells grew as monolayers with a population doubling time of 74 h during exponential growth at Passage 25. They had an epithelial-like morphology and were positive for mucine staining. Ultrastructural studies revealed the presence of microvilli on the cell surface and poorly developed organelles in the cytoplasm. The HuCC-T1 cell was tumorigenic in nude mice. The number of chromosomes in HuCC-T1 ranged from 61 to 80. These human cholangiocellular carcinoma cells in serum-free medium secreted several tumor markers, including carbohydrate antigen 19/9, carbohydrate antigen 125, carcinoembryonic antigen, and tissue polypeptide antigen. The carbohydrate antigen 19/9 secretion level of HuCC-T1 cells cultured in RPMI 1640 medium with 1% fetal bovine serum was sixfold higher than that with 0.2% lactalbumin hydrolysate. These findings suggest that HuCC-T1 will provide useful information to clarify the mechanism of tumor marker secretion and tumor cell growth in the human cholangiocellular carcinoma.

  4. Comparison between two treatment protocols with recombinant Human Erythropoietin (rHuEpo in the treatment of late anemia in neonates with Rh-Isoimmunization

    Directory of Open Access Journals (Sweden)

    A.A. Zuppa

    2012-08-01

    Full Text Available Objectve. The Rh-hemolytic disease can lead to a late anemia by hemolytic and hyporigenerative mechanism. We compared the effectiveness of rHuEPO in two care protocols that differ for doses of rHuEPO administrated and for timing of administration. Methods. A cohort of 14 neonates was investigated. The neonates were treated with two different protocols. Protocol A: a dose of 200 U/kg/day of rHuEpo administered subcutaneously starting from the end of the second week of life; Protocol B: a dose of 400 U/kg/day of rHuEpo administered subcutaneously starting from the end of the first week of life. Results. The hematocrit values in the protocol A group decreased during treatment (32,5% vs 25,2%, whereas the hematocrit value in protocol B group remained almost stable (38,7% vs 42,8%. The mean numbers of platelets remained stable in both groups while neutrophils increased in protocol A group and decreased in protocol B (p<0,05. Reticulocyte count increased during treatment in both groups, although only in protocol B group it was statistically significative (p<0,05. Conclusions. Our results suggest a similar efficacy between the two treatment protocols. Increasing doses of rHuEPO do not seem enhancing their effectiveness and the incidence of side effects.

  5. Pharmacological Progress of HuZhang%中药虎杖药理作用研究进展

    Institute of Scientific and Technical Information of China (English)

    黄海量

    2012-01-01

    总结近年来关于中药虎杖中有效成分治疗心血管系统、消化系统、呼吸系统、内分泌系统、生殖系统、免疫系统等疾病的文献报道,发现虎杖含有的丰富活性成分具有多项药理作用,临床应用日益广泛,但其毒理研究和有效成分提取方法的报道鲜见.%Literature and reports on effective constituents of HuZhang (Polygonum cuspidatum) in treating the diseases of cardiovascular system, digestive system, respiratory system, endocrine system, reproductive system, immune system and so on were concluded in recent years and the result of study showed that rich active constituents in HuZhang had pharmacological functions and were widely used day by day, but toxicological studies and extraction of effective constituent were rarely reported.

  6. Optimization and risk analyses for rule curves of reservoir operation: application to Tien-Hua-Hu Reservoir in Taiwan.

    Science.gov (United States)

    Kuo, J T; Hsu, N S; Chiu, S K

    2006-01-01

    Tien-Hua-Hu Reservoir is currently under planning by the Water Resources Agency, Taiwan to meet the increasing water demands of central Taiwan arising from rapid growth of domestic water supply, and high-tech industrial parks. This study develops a simulation model for the ten-day period reservoir operation to calculate the ten-day water shortage index under varying rule curves. A genetic algorithm is coupled to the simulation model to find the optimal rule curves using the minimum ten-day water shortage index as an objective function. This study generates many sets of synthetic streamflows for risk, reliability, resiliency, and vulnerability analyses of reservoir operation. ARMA and disaggregation models are developed and applied to the synthetic streamflow generation. The optimal rule curves obtained from this study perform better in the ten-day shortage index when compared to the originally designed rule curves from a previous study. The optimal rule curves are also superior to the originally designed rule curves in terms of vulnerability. However, in terms of reliability and resiliency, the optimal rule curves are inferior to the those originally designed. Results from this study have provided in general a set of improved rule curves for operation of the Tien-Hua-Hu Reservoir. Furthermore, results from reliability, resiliency and vulnerability analyses offer much useful information for decision making in reservoir operation.

  7. r-huEPO在胃肠道恶性肿瘤患者围手术期应用

    Institute of Scientific and Technical Information of China (English)

    缪传文; 李继坤; 温玉刚; 周立生; 李旭

    2006-01-01

    胃肠道恶性肿瘤往往伴有程度不等的贫血,以往临床上除了输血疗效确切外,只有使用各种生血制剂,主要是铁剂,往往起效缓慢,难以令人满意。而输血由于血源紧张,还有各种输血的并发症,及传播各种严重传染病性疾病的危险,使用受到限制。重组人促红细胞生成素(recombinant erythropoietin)的围手术期红细胞动员也越来越受到临床重视,有研究表现大剂量应用r-huEPO有明显的红细胞动员作用。2003年-2005年,我们应用r-huEPO治疗胃肠道恶性肿瘤伴贫血的手术患者276例,取得了较好的疗效。

  8. Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell

    Directory of Open Access Journals (Sweden)

    Aris Haryanto

    2015-10-01

    Full Text Available The application of fluorescent proteins as expression markers and protein fusion partners has provedimmensely valuable for resolving the organization of biological events in living cells. EGFP and DsRed2 arecommonly fluorescent marker protein which is used for biotechnology and cell biology research. The presentstudy was designed to identify the expression vector that suitable to ligate with DNA encoding HBV coreprotein for intracellular localization study in hepatocyte cell, which were expressed as fusion proteins. We alsocompared and quantified the expressed fluorescent protein which predominantly localized in the cellcompartment. The results indicated that DsRed2 shown as less than ideal for intracellular localization study ofthan EGFP, because of its tetrameric structure of the fluorescent protein and when fused to a protein of interest,the fusion protein often forms aggregates in the living cells. In contrast, EGFP fluorescent protein shown a muchhigher proportion of cytoplasmic localization, thus being more suitable for analysis of intracellular localizationthan DsRed2 fluorescent protein. EGFP fluorescent protein is also capable to produce a strong green fluorescencewhen excited by blue light, without any exogenously added substrate or cofactor, events inside living cell canthus be visualized in a non-invasive way. Based on our present quantitative data and some reasons above shownthat EGFP is more suitable than DsRed2 as a fluorescent marker protein for intracellular localization study intoHuH-7 cell.Keywords: EGFP, DsRed2 fluorescent protein , HuH-7 cell, HBV, intracellular localization

  9. Mouse monocyte/macrophage cell line J774A.1 migration induced by the activation of CB1 depends on HuR%激活大麻素受体1诱导的单核巨噬细胞J774A.1的迁移依赖RNA结合蛋白HuR

    Institute of Scientific and Technical Information of China (English)

    赵中新; 常娜; 盖菁菁; 田蕾; 李丽英

    2016-01-01

    目的 研究大麻素受体1(cannabinoid receptor 1,CB1)在单核巨噬细胞迁移中的重要作用以及RNA结合蛋白人抗原R (human antigen R,HuR)参与其中的可能机制.方法 选用单核巨噬细胞系J774A.1,应用琼脂糖凝胶电泳和免疫荧光染色技术鉴定J774A.1中CB1以及HuR的表达;ACEA和AM281分别为CB1的药理学激动剂和拮抗剂,应用Boyden chamber法检测ACEA和AM281对J774A.1迁移活性的影响.HuR的基因干扰用于确定激活CB1诱导的J774A.1迁移功能是否依赖HuR;胞质蛋白的分离用于探究激活CB1是否能引起J774A.1胞质中HuR的富集;RT-qPCR和Western blotting法检测CB1和HuR mRNA和蛋白质的变化情况.结果 该研究证明J774A.1在基因和蛋白质水平上均表达CB1和HuR;激活CB1能够促进J774A.1的迁移(P<0.01)并且能够被其药理学拮抗剂AM281所抑制;激活CB1诱导的J774A.1的迁移依赖HuR;激活CB1促进了J774A.1胞质中HuR的富集进一步影响了CB1的表达,由此HuR参与了激活CB1诱导的J774A.1的迁移.结论 激活CB1能够诱导单核巨噬细胞系J774A.1的迁移,且此过程依赖RNA结合蛋白HuR.

  10. Human-derived IgG level as an indicator for EBV-associated lymphoma model in Hu-PBL/SCID chimeras.

    Science.gov (United States)

    Tang, Yunlian; He, Rongfang; Zhang, Yang; Liu, Fang; Cheng, Ailan; Wu, Yimou; Gan, Runliang

    2011-05-09

    Epstein-Barr virus (EBV) has a close association with various types of human lymphomas. Animal models are essential to elucidate the pathogenesis of human EBV-associated lymphomas. The aim of the present study is to evaluate the association between human IgG concentration and EBV-associated lymphoma development in huPBL/SCID mice. Human peripheral blood lymphocytes (hu-PBL) from EBV-seropositive donors were inoculated intraperitoneally into SCID mouse. Immunohistochemical staining was used to examine differentiated antigens of tumor cells. EBV infection of the induced tumors was detected by in situ hybridization. IgG concentrations in the serums of 12 SCID mice were measured by unidirectional immunodiffusion assay. 21 out of 29 mice developed tumors in their body. Immunohistochemical staining showed that all induced tumors were LCA (leukocyte common antigen) positive, B-cell markers (CD20, CD79a) positive, and T-cell markers (both CD3 and CD45RO) negative. The tumors can be diagnosed as human B-cell lymphomas by these morphological and immunohistochemical features. In situ hybridization exhibited resultant tumor cells had EBV encoded small RNA-1 (EBER-1). Human-derived IgG could be found in the serum from SCID mice on the 15th day following hu-PBL transplantation, and IgG levels increased with the tumor development in 6 hu-PBL/SCID chimeras. Intraperitoneal transfer of hu-PBLs from EBV+ donors to SCID mice leads to high human IgG levels in mouse serum and B cell lymphomas. Our findings suggest that increasing levels of human-derived IgG in peripheral blood from hu-PBL/SCID mice could be used to monitor EBV-related human B-cell lymphoma development in experimental animals.

  11. Development and Characterization of a Humanized Anti-HER2 Antibody HuA21 with Potent Anti-Tumor Properties in Breast Cancer Cells.

    Science.gov (United States)

    Li, Ruilin; Hu, Siyi; Chang, Yan; Zhang, Zhihui; Zha, Zhao; Huang, Hui; Shen, Guodong; Liu, Jing; Song, Lihua; Wei, Wei

    2016-04-15

    Human epidermal growth factor receptor 2 (HER2) is one of the most studied tumor-associated antigens for cancer immunotherapy. An engineered anti-HER-2 chimeric A21 antibody (chA21) is a chimeric antibody targeted to subdomain I of the HER2 extracellular domain. Here, we report the anti-tumor activity of the novel engineered monoclonal antibody humanized chA21 (HuA21) that targets HER2 on the basis of chA21, and we describe the underlying mechanisms. Our results reveal that HuA21 markedly inhibits the proliferation and migration of HER2-overexpressing breast cancer cells and causes enhanced antibody-dependent cell-mediated cytotoxicity potency against HER2-overexpressing tumor cells. In particular, HuA21, but not trastuzumab (Tra), markedly suppresses growth and enhances the internalization of the antibody in Tra-resistant BT-474 breast cancer cells. These characteristics are highly associated with the intrinsic ability of HuA21 to down-regulate HER2 activation and inhibit the extracellular signal-regulated kinase 1/2 (ERK1/2) and protein kinase B (Akt) signaling pathways. Furthermore, the combination of HuA21 with Tra synergistically enhances the anti-tumor effects in vitro and in vivo and inhibits HER2 activation and the ERK1/2 and Akt signaling pathways. Altogether, our results suggest that HuA21 may represent a unique anti-HER2 antibody with potential as a therapeutic candidate alone or in combination with other anti-HER2 reagents in cancer therapy.

  12. Development and Characterization of a Humanized Anti-HER2 Antibody HuA21 with Potent Anti-Tumor Properties in Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Ruilin Li

    2016-04-01

    Full Text Available Human epidermal growth factor receptor 2 (HER2 is one of the most studied tumor-associated antigens for cancer immunotherapy. An engineered anti-HER-2 chimeric A21 antibody (chA21 is a chimeric antibody targeted to subdomain I of the HER2 extracellular domain. Here, we report the anti-tumor activity of the novel engineered monoclonal antibody humanized chA21 (HuA21 that targets HER2 on the basis of chA21, and we describe the underlying mechanisms. Our results reveal that HuA21 markedly inhibits the proliferation and migration of HER2-overexpressing breast cancer cells and causes enhanced antibody-dependent cell-mediated cytotoxicity potency against HER2-overexpressing tumor cells. In particular, HuA21, but not trastuzumab (Tra, markedly suppresses growth and enhances the internalization of the antibody in Tra-resistant BT-474 breast cancer cells. These characteristics are highly associated with the intrinsic ability of HuA21 to down-regulate HER2 activation and inhibit the extracellular signal-regulated kinase 1/2 (ERK1/2 and protein kinase B (Akt signaling pathways. Furthermore, the combination of HuA21 with Tra synergistically enhances the anti-tumor effects in vitro and in vivo and inhibits HER2 activation and the ERK1/2 and Akt signaling pathways. Altogether, our results suggest that HuA21 may represent a unique anti-HER2 antibody with potential as a therapeutic candidate alone or in combination with other anti-HER2 reagents in cancer therapy.

  13. HIV-1 Pathogenesis and Therapeutic Intervention in the SCID-hu Thy/Liv Mouse: A Model for Primary HIV-1 Infection in the Human Thymus

    OpenAIRE

    Su, Lishan

    1997-01-01

    The SCID-hu Thy/Liv mouse is a model for the analysis of human thymopoiesis. It has been constructed by engrafting fragments of human fetal liver and thymus into the immunodeficient C.B-17 scid/scid (SCID) mouse. The resulting ‘Thy/Liv’ organ promotes long-term differentiation of human T cells. Given the apparently normal physiology of the SCID-hu Thy/Liv organ, it has been used to explore the pathophysiologic mechanisms of HIV-1 infection in vivo, and to test therapeutic modalities such as a...

  14. HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-γc-/- (RAG-hu mouse model

    Directory of Open Access Journals (Sweden)

    Connick Elizabeth

    2006-11-01

    Full Text Available Abstract Background The currently well-established humanized mouse models, namely the hu-PBL-SCID and SCID-hu systems played an important role in HIV pathogenesis studies. However, despite many notable successes, several limitations still exist. They lack multi-lineage human hematopoiesis and a functional human immune system. These models primarily reflect an acute HIV infection with rapid CD4 T cell loss thus limiting pathogenesis studies to a short-term period. The new humanized Rag2-/-γc-/- mouse model (RAG-hu created by intrahepatic injection of CD34 hematopoietic stem cells sustains long-term multi-lineage human hematopoiesis and is capable of mounting immune responses. Thus, this model shows considerable promise to study long-term in vivo HIV infection and pathogenesis. Results Here we demonstrate that RAG-hu mice produce human cell types permissive to HIV-1 infection and that they can be productively infected by HIV-1 ex vivo. To assess the capacity of these mice to sustain long-term infection in vivo, they were infected by either X4-tropic or R5-tropic HIV-1. Viral infection was assessed by PCR, co-culture, and in situ hybridization. Our results show that both X4 and R5 viruses are capable of infecting RAG-hu mice and that viremia lasts for at least 30 weeks. Moreover, HIV-1 infection leads to CD4 T cell depletion in peripheral blood and thymus, thus mimicking key aspects of HIV-1 pathogenesis. Additionally, a chimeric HIV-1 NL4-3 virus expressing a GFP reporter, although capable of causing viremia, failed to show CD4 T cell depletion possibly due to attenuation. Conclusion The humanized RAG-hu mouse model, characterized by its capacity for sustained multi-lineage human hematopoiesis and immune response, can support productive HIV-1 infection. Both T cell and macrophage tropic HIV-1 strains can cause persistent infection of RAG-hu mice resulting in CD4 T cell loss. Prolonged viremia in the context of CD4 T cell depletion seen in this

  15. Commentary of Hu Yihong' s Animation Theory%胡依红动画理论述评

    Institute of Scientific and Technical Information of China (English)

    屈立丰

    2012-01-01

    本文对上海美影厂女动画家胡依红的动画理论思想与动画创作实践做了简单梳理,细致分析了其“动画创新论”和“动画本体论”的理论来源和框架构成,对其动画理论思想和动画实践之间的互动关系进行了初步讨论。%This paper gave a brief introduction of the animation theory and creations by Hu Yihong, a female animator from Shanghai Animation Film Studio, made a detailed analysis of the sources and framework of her "ani- mation innovation theory" and "animation ontology", and finally presented a tentative discussion about the interac- tion between her ideology of animation theory and animation creation.

  16. A New Arthropod, Guangweicaris Luo, Fu et Hu gen. nov.from the Early Cambrian Guanshan Fauna, Kunming, China

    Institute of Scientific and Technical Information of China (English)

    LUO Huilin; FU Xiaoping; HU Shixue; LI Yong; HOU Shuguang; YOU Ting; PANG Jiyuan; LIU Qi

    2007-01-01

    The Guanshan Fauna is a soft-bodied fauna dominated by arthropods (including trilobites,trilobitoides, Tuzoia, Isoxys, and bradorids) in association with priapulids, brachiopods,anomalocaridids, vetulicoliids, sponges, chancellorids, and echinoderms. This paper reports and describes a new arthropod from the yellowish green mudstone at the lower part of the Wulongqing Formation, Canglangpuan Stage, Lower Cambrian in Kunming, Yunnan, China. The stratigraphic and geographic distribution, classification, fossil preservation, life style of this new arthropod and comparisons with other fossil arthropods are also discussed in details. The discovery and research of the non-mineralized arthropod, Guangweicaris Luo, Fu et Hu gen. nov. from the Guanshan Fauna adds new members to the taxonomic list and provides new information to the evolution of early arthropods.Furthermore, this study would shed new light into the "Cambrian Explosion" and the evolution of early life.

  17. Chinese President Hu Jintao and Austrian President Heinz Fischer Send Messages of Congratulations on 2006 China Year in Austria

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    <正>Editor’s note: In January 2004, Benita Ferrero-Waldner, Austrian foreign minister at the time, announced that China Year would be held in Austria in 2006 to mark the 35th anniversary of the establishment of diplomatic relations between Austria and China and to promote understanding of China and bilateral cooperation. The two sides decided after consultation that the activities for the China Year would be jointly organized by the Austria-China Friendship Association and the Chinese People’s Association for Friendship with Foreign Countries. The governments of the two countries attach great importance to the holding of the China Year. Chinese President Hu Jintao, Austrian President Heinz Fischer and Federal Chancellor Wolfgang Schussel and other state leaders

  18. Effects of vesicular-arbuscular mycorrhizae on the drought resistance of wild jujube (Zizyphs spinosus Hu) seedlings

    Institute of Scientific and Technical Information of China (English)

    LU Jinying; LIU Min; MAO Yongmin; SHEN Lianying

    2007-01-01

    The current study explored the effects of vesicular-arbuscular mycorrhizae (VAM) inoculation on the growth and water requirement of pot-grown wild jujube (Zizyphs spinosus Hu).Three water regimes (20%,40% and 60% of soil water content) were conducted.The VAM inoculation could significantly increase plant growth (including plant height,leaf area,and fresh and dry mass),enhance relative leaf water content,photosynthetic rates,transpiration rates and stomatal conductance,and improve plant drought tolerance.The water consumption of the mycorrhizal plants producing 1 g of dry matter was 18.7%-26.6% lower than the consumption of non-mycorrhizal plants grown under the same soil water content conditions.

  19. A computational screen for site selective A-to-I editing detects novel sites in neuron specific Hu proteins

    Directory of Open Access Journals (Sweden)

    Furey Terrence S

    2010-01-01

    Full Text Available Abstract Background Several bioinformatic approaches have previously been used to find novel sites of ADAR mediated A-to-I RNA editing in human. These studies have discovered thousands of genes that are hyper-edited in their non-coding intronic regions, especially in alu retrotransposable elements, but very few substrates that are site-selectively edited in coding regions. Known RNA edited substrates suggest, however, that site selective A-to-I editing is particularly important for normal brain development in mammals. Results We have compiled a screen that enables the identification of new sites of site-selective editing, primarily in coding sequences. To avoid hyper-edited repeat regions, we applied our screen to the alu-free mouse genome. Focusing on the mouse also facilitated better experimental verification. To identify candidate sites of RNA editing, we first performed an explorative screen based on RNA structure and genomic sequence conservation. We further evaluated the results of the explorative screen by determining which transcripts were enriched for A-G mismatches between the genomic template and the expressed sequence since the editing product, inosine (I, is read as guanosine (G by the translational machinery. For expressed sequences, we only considered coding regions to focus entirely on re-coding events. Lastly, we refined the results from the explorative screen using a novel scoring scheme based on characteristics for known A-to-I edited sites. The extent of editing in the final candidate genes was verified using total RNA from mouse brain and 454 sequencing. Conclusions Using this method, we identified and confirmed efficient editing at one site in the Gabra3 gene. Editing was also verified at several other novel sites within candidates predicted to be edited. Five of these sites are situated in genes coding for the neuron-specific RNA binding proteins HuB and HuD.

  20. Validation of the SCID-hu Thy/Liv mouse model with four classes of licensed antiretrovirals.

    Directory of Open Access Journals (Sweden)

    Cheryl A Stoddart

    Full Text Available BACKGROUND: The SCID-hu Thy/Liv mouse model of HIV-1 infection is a useful platform for the preclinical evaluation of antiviral efficacy in vivo. We performed this study to validate the model with representatives of all four classes of licensed antiretrovirals. METHODOLOGY/PRINCIPAL FINDINGS: Endpoint analyses for quantification of Thy/Liv implant viral load included ELISA for cell-associated p24, branched DNA assay for HIV-1 RNA, and detection of infected thymocytes by intracellular staining for Gag-p24. Antiviral protection from HIV-1-mediated thymocyte depletion was assessed by multicolor flow cytometric analysis of thymocyte subpopulations based on surface expression of CD3, CD4, and CD8. These mice can be productively infected with molecular clones of HIV-1 (e.g., the X4 clone NL4-3 as well as with primary R5 and R5X4 isolates. To determine whether results in this model are concordant with those found in humans, we performed direct comparisons of two drugs in the same class, each of which has known potency and dosing levels in humans. Here we show that second-generation antiretrovirals were, as expected, more potent than their first-generation predecessors: emtricitabine was more potent than lamivudine, efavirenz was more potent than nevirapine, and atazanavir was more potent than indinavir. After interspecies pharmacodynamic scaling, the dose ranges found to inhibit viral replication in the SCID-hu Thy/Liv mouse were similar to those used in humans. Moreover, HIV-1 replication in these mice was genetically stable; treatment of the mice with lamivudine did not result in the M184V substitution in reverse transcriptase, and the multidrug-resistant NY index case HIV-1 retained its drug-resistance substitutions. CONCLUSION: Given the fidelity of such comparisons, we conclude that this highly reproducible mouse model is likely to predict clinical antiviral efficacy in humans.

  1. Dieta de roedores sigmodontinos (Cricetidae en los bosques montanos tropicales de Huánuco, Perú

    Directory of Open Access Journals (Sweden)

    Maggie C. Noblecilla

    2013-06-01

    Full Text Available Se analizó el contenido estomacal de cinco especies de roedores sigmodontinos: Akodon orophilus, Microry-zomys altissimus, M. minutus, Thomasomys notatus yT. kalinowskii, procedentes de los bosques montanos de Huánuco, Perú (2564 - 3850 m de altitud. Concluimos que A. orophiluses insectívora por haber presentado un alto volumen de artrópodos (adultos y larvas en el contenido estomacal (90,1%; mientras que T. notatusy T. kalinowskiison principalmente herbívoras por el alto volumen de materia vegetal, 89% y 67,75% respectiva-mente; y que M. altissimusy M. minutusson omnívoras por presentar volúmenes similares tanto para vegetales como para artrópodos. Thomasomys kalinowskiies considerada generalista por la mayor amplitud de nicho (4,61, mientras A. orophiluses considerada especialista por el menor valor (1,70. Akodon orophilusmostró una preferencia por el consumo de artrópodos adultos al tener un bajo coeficiente de variación (CV= 20% y también un significativo aumento en el consumo de larvas de artrópodos en la época húmeda, siendo la única especie con variación estacional en la dieta. Por otro lado, la sobreposición de nicho fue menor a 0,75 en el 80% de los pares de especies comparados, indicando una baja similitud en la dieta. La mayor similitud en la dieta se observó entre M. altissimus- T. notatus(0,822 y M. minutus - T. kalinowskii(0,816. Se concluye que estos roedores sigmodontinos, simpátricos en los bosques montanos de Huánuco, exhiben dietas disímiles, probablemente como una estrategia para evitar o aminorar la competencia interespecífica.

  2. Validation of the SCID-hu Thy/Liv mouse model with four classes of licensed antiretrovirals.

    Science.gov (United States)

    Stoddart, Cheryl A; Bales, Cheryl A; Bare, Jennifer C; Chkhenkeli, George; Galkina, Sofiya A; Kinkade, April N; Moreno, Mary E; Rivera, José M; Ronquillo, Rollie E; Sloan, Barbara; Black, Paul L

    2007-08-01

    The SCID-hu Thy/Liv mouse model of HIV-1 infection is a useful platform for the preclinical evaluation of antiviral efficacy in vivo. We performed this study to validate the model with representatives of all four classes of licensed antiretrovirals. Endpoint analyses for quantification of Thy/Liv implant viral load included ELISA for cell-associated p24, branched DNA assay for HIV-1 RNA, and detection of infected thymocytes by intracellular staining for Gag-p24. Antiviral protection from HIV-1-mediated thymocyte depletion was assessed by multicolor flow cytometric analysis of thymocyte subpopulations based on surface expression of CD3, CD4, and CD8. These mice can be productively infected with molecular clones of HIV-1 (e.g., the X4 clone NL4-3) as well as with primary R5 and R5X4 isolates. To determine whether results in this model are concordant with those found in humans, we performed direct comparisons of two drugs in the same class, each of which has known potency and dosing levels in humans. Here we show that second-generation antiretrovirals were, as expected, more potent than their first-generation predecessors: emtricitabine was more potent than lamivudine, efavirenz was more potent than nevirapine, and atazanavir was more potent than indinavir. After interspecies pharmacodynamic scaling, the dose ranges found to inhibit viral replication in the SCID-hu Thy/Liv mouse were similar to those used in humans. Moreover, HIV-1 replication in these mice was genetically stable; treatment of the mice with lamivudine did not result in the M184V substitution in reverse transcriptase, and the multidrug-resistant NY index case HIV-1 retained its drug-resistance substitutions. Given the fidelity of such comparisons, we conclude that this highly reproducible mouse model is likely to predict clinical antiviral efficacy in humans.

  3. Infección por Gnathostoma (Spirurida: Gnathostomatidae en Hoplias microlepis: prevalencia, correlación con la talla del pez, huéspedes e implicaciones para salud pública en Ecuador

    Directory of Open Access Journals (Sweden)

    Juan José Alava

    2009-12-01

    Conclusiones. La gnathostomiasis sigue siendo prevalente en la zona costera de Ecuador y el agente patógeno es aún encontrado en el huésped intermediario. Diversos mamíferos neotropicales estarían actuando como huésped reservorio definitivo en el ciclo biológico de Gnathostoma en Ecuador.

  4. FengYin Tang in Treating 47 Patients with Perimenopause of Liver-Yang Hyperactivity Pattern%风引汤治疗肝阳上亢型绝经综合征47例

    Institute of Scientific and Technical Information of China (English)

    李江慧; 曹保利

    2014-01-01

    Objective:To survey therapeutic effects of modified FengYin Tang in treating perimenopause of liver-Yang hyperactivity pattern. Methods:Ninety-four patients were allocated to the observation group and the con-trol group randomly, 47 cases of the observation group took modified FengYin Tang and the control group Xin-ShenAn capsule. Curative effects of both groups, the improvements of TCM syndrome, the changes of follicle-stimu-lating hormone (FSH), leuteinizing hormone (LH) and estradiol (E2) were observed. Results:Total effective rate of the observation group was 93.62%, higher than 80.85%of the control group, the difference had statistical meaning (P0.05). After treating, the levels of E2, FSH and LH in both groups were notably improved (P0.05), the difference had statistical meaning in FSH and E2 after treating (P<0.05). Conclusion:FengYin Tang indicates the advantages of improving the symptoms and internal secretion of the patients suffering from perimenopause of liver-Yang hyperactivity pattern without obvious adverse reaction.%目的:观察风引汤加减治疗肝阳上亢型绝经综合征的临床疗效。方法:选择94例肝阳上亢型绝经综合征门诊患者,随机分为观察组与对照组。观察组47例给予风引汤加减治疗,对照组47例给予心神安胶囊。观察2组临床疗效、中医证候的改善情况及血清中卵泡刺激素(FSH)、促黄体生成激素(LH)及雌二醇(E2)的水平变化。结果:总有效率观察组为93.62%,对照组为80.85%,2组比较差异有统计学意义(P<0.05);观察组改善烘热汗出、烦躁易怒、眩晕耳鸣、胁肋胀满、皮肤干燥、阴道干涩、悲伤欲哭、口苦咽干等症状的疗效优于对照组(P<0.05);对照组改善腰膝酸软的症状疗效优于观察组(P<0.05);其余症状2组比较,差异无统计学意义(P>0.05)。治疗后E2、FSH、LH水平2组均明显改善(P<0.05);LH治疗后组间比较,差异无统计学意义(P>0

  5. The State of Harmony Constituted by Four Hearts on Feng Zikai's Aesthetic Ideal%“四心”圆融之境:丰子恺审美理想论

    Institute of Scientific and Technical Information of China (English)

    蒋霞; 杨晓河

    2014-01-01

    If we want to understand Feng Zikai's aesthetic view,we need understand the metaphor of the “three-story building”about reality,art and religion.The layer of reality is the foundation and the ultimate purport;the layer of art has double identities of ontological significance and pivotal role;and the layer of religion is the highest state and has spiritual leading meaning to the below layers.Feng Zi-kai considers only religion can reveal the most profound meaning of “the true”.“The good”is a com-bination of compassion of Buddhism and Confucian benevolence.“The beautiful”contains the beauty of the form which has nothing to do with the content and the beauty of the spirit which is based on “the true“and “the good”.The latter is higher than the former.The beauty of spirit has the implication of Buddhism.The heart of art is associated with the heart of religion.At the same time the heart of art is associated with the heart of benevolence and the heart of childlike innocence.The four hearts are inter-linked with each other to the state of harmony,which constitutes Feng Zikai's unique aesthetic ideal.%对丰子恺的审美观的认识,需要放在现实∕艺术∕宗教的“三层楼”的比喻中。现实层是基础,亦是最终旨归,艺术层兼有本体意义与枢纽作用双重身份,宗教层是至高境界,对下具有精神引领的作用。丰子恺认为,对“真”的最深刻的揭示惟有宗教能够担当;“善”是佛教之慈悲与儒家之仁的结合;“美”包含与内容无涉的形式之美以及以真善为底子的精神之美,后者高于前者,精神之美具有佛教的蕴涵。艺术心与宗教心相沟通,同时还与仁心、童心相沟通,“四心”相通达至圆融之境,构成了丰子恺独特的审美理想。

  6. HuCNS-SC Human NSCs Fail to Differentiate, Form Ectopic Clusters, and Provide No Cognitive Benefits in a Transgenic Model of Alzheimer's Disease

    Directory of Open Access Journals (Sweden)

    Samuel E. Marsh

    2017-02-01

    Full Text Available Transplantation of neural stem cells (NSCs can improve cognition in animal models of Alzheimer's disease (AD. However, AD is a protracted disorder, and prior studies have examined only short-term effects. We therefore used an immune-deficient model of AD (Rag-5xfAD mice to examine long-term transplantation of human NSCs (StemCells Inc.; HuCNS-SCs. Five months after transplantation, HuCNS-SCs had engrafted and migrated throughout the hippocampus and exhibited no differences in survival or migration in response to β-amyloid pathology. Despite robust engraftment, HuCNS-SCs failed to terminally differentiate and over a quarter of the animals exhibited ectopic human cell clusters within the lateral ventricle. Unlike prior short-term experiments with research-grade HuCNS-SCs, we also found no evidence of improved cognition, no changes in brain-derived neurotrophic factor, and no increase in synaptic density. These data, while disappointing, reinforce the notion that individual human NSC lines need to be carefully assessed for efficacy and safety in appropriate long-term models.

  7. Short communication. Employment of molecular markers to develop tetraploid “supermale” asparagus from andromonoecious plants of the landrace ‘Morado de Huétor’

    Directory of Open Access Journals (Sweden)

    Jose J. Regalado

    2014-10-01

    Full Text Available The aim of this work was the development of new “supermale” genotypes retaining the highest genetic diversity possible of the tetraploid asparagus landrace ‘Morado de Huétor’. The elite andromonoecious male HT664 of the ‘Morado de Huétor’ asparagus landrace and the andromonoecious hybrid male HC027, obtained by crossing between this landrace and a commercial cultivar of Asparagus officinalis, were selected for self-pollination to produce possible “supermales” with genes of ‘Morado de Huétor’ (SMHT. To confirm the hybrid nature of HC027, we characterized this genotype with EST-SSR (Expressed Sequence Tag-derived Simple Sequence Repeats markers. We also adopted the sex-linked marker Asp1-T7 for sex determination in ‘Morado de Huétor’ and the resulting hybrids between this landrace and other commercial cultivars. Asp1-T7 marker was used for the selection and genotyping of SMHT. “All-male” cultivars with a different genetic background can be generated by crossing females with these new “supermale” genotypes, and the agronomical traits of these new cultivars would be very different from the “all-male” cultivars currently available in the market, making them extremely interesting for asparagus breeding programs.

  8. On the Theoretical Character of Hu Jintao's Youth Viewpoint%胡锦涛同志青年观的理论品格思想研究

    Institute of Scientific and Technical Information of China (English)

    邹洪伟

    2009-01-01

    Mr. Hu Jintao has delivered lots pithy opinions on the youth work and the healthy growth of Chi-nese youths, with which Mr. Hu has developed the Marxist youth theory to its new level. This paper makes a brief illustration of the guidelines, the kernel, the goal and the historical mission of the youth work and the healthy growth of Chinese youths mentioned by Mr. Hu, trying to discuss the theoretical character of Mr. Hu Jintao's youth viewpoint.%胡锦涛同志对中国青年成长成才和青年工作提出了诸多精辟的观点,开拓了马克思主义青年观的新境界.本文就其中青年成长成才和青年工作的指导思想、核心、努力目标和历史使命等问题进行了简要阐述,充分展现胡锦涛同志青年观的理论品格.

  9. Purchase of Microwave Reactors for Implementation of Small-scale Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate Curriculum and Synthetic Chemistry Research at HU

    Science.gov (United States)

    2015-05-16

    Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate Curriculum and Synthetic Chemistry Research at HU The views, opinions and/or...Research Triangle Park, NC 27709-2211 Microwave laboratory facility, Undergraduate organic chemistry laboratory, Implementation/Development of...Purchase of Microwave Reactors for Implementation of Small-scale Microwave-accelerated Organic Chemistry Laboratory Program in Undergraduate

  10. HuD promotes BDNF expression in brain neurons via selective stabilization of the BDNF long 3'UTR mRNA.

    Directory of Open Access Journals (Sweden)

    Megan Allen

    Full Text Available Complex regulation of brain-derived neurotrophic factor (BDNF governs its intricate functions in brain development and neuronal plasticity. Besides tight transcriptional control from multiple distinct promoters, alternative 3'end processing of the BDNF transcripts generates either a long or a short 3'untranslated region (3'UTR. Previous reports indicate that distinct RNA sequence in the BDNF 3'UTRs differentially regulates BDNF production in the brain to accommodate neuronal activity changes, conceivably through differential interactions with undefined trans-acting factors that regulate stability and translation of these BDNF mRNA isoforms. In this study, we report that the neuronal RNA-binding protein (RBP HuD interacts with a highly conserved AU-rich element (ARE specifically located in the BDNF long 3'UTR. Such interaction is necessary and sufficient for selective stabilization of mRNAs that contain the BDNF long 3'UTR in vitro and in vivo. Moreover, in a HuD transgenic mouse model, the BDNF long 3'UTR mRNA is increased in the hippocampal dentate granule cells (DGCs, leading to elevated expression of BDNF protein that is transported and stored in the mossy fiber (MF terminals. Our results identify HuD as the first trans-acting factor that enhances BDNF expression specifically through the long 3'UTR and a novel mechanism that regulates BDNF protein production in selected neuronal populations by HuD abundance.

  11. The problem of the ethical root of truth (A tribute to Antonio Rodríguez Huéscar on his birth centenary

    Directory of Open Access Journals (Sweden)

    Juan Padilla

    2013-12-01

    Full Text Available This paper deals with the meaning of truth as «coincidence of man with himself» accordingto Ortega y Gasset and his disciple rodríguez huéscar. What the concept does not mean and which implications it has are also explained.

  12. PARP1 promotes gene expression at the post-transcriptional level by modulating the RNA-binding protein HuR

    Science.gov (United States)

    Ke, Yueshuang; Han, Yanlong; Guo, Xiaolan; Wen, Jitao; Wang, Ke; Jiang, Xue; Tian, Xue; Ba, Xueqing; Boldogh, Istvan; Zeng, Xianlu

    2017-01-01

    Poly(ADP-ribosyl)ation (PARylation) is mainly catalysed by poly-ADP-ribose polymerase 1 (PARP1), whose role in gene transcription modulation has been well established. Here we show that, in response to LPS exposure, PARP1 interacts with the adenylateuridylate-rich element-binding protein embryonic lethal abnormal vision-like 1 (Elavl1)/human antigen R (HuR), resulting in its PARylation, primarily at site D226. PARP inhibition and the D226 mutation impair HuR's PARylation, nucleocytoplasmic shuttling and mRNA binding. Increases in mRNA level or stability of pro-inflammatory cytokines/chemokines are abolished by PARP1 ablation or inhibition, or blocked in D226A HuR-expressing cells. The present study demonstrates a mechanism to regulate gene expression at the post-transcriptional level, and suggests that blocking the interaction of PARP1 with HuR could be a strategy to treat inflammation-related diseases that involve increased mRNA stability. PMID:28272405

  13. Priming with r-metHuSCF and filgrastim or chemotherapy and filgrastim in patients with malignant lymphomas: a randomized phase II pilot study of mobilization and engraftment

    DEFF Research Database (Denmark)

    Johnsen, Hanne; Geisler, C; Juvonen, E

    2011-01-01

    SCF has been shown to synergize with G-CSF to mobilize CD34(+) PBPCs. In this study we report results from this combination after a phase II trial of 32 patients with malignant lymphoma randomized to receive recombinant methionyl human SCF (ancestim, r-metHuSCF) in combination with recombinant me...

  14. Investigation of the HU-density conversion method and comparison of dose distribution for dose calculation on MV cone beam CT images

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Min Joo; Lee, Seu Ran; Suh, Tae Suk [Dept. of Biomedical Engineering, The Catholic University of Korea, Bucheon (Korea, Republic of)

    2011-11-15

    Modern radiation therapy techniques, such as Image-guided radiation therapy (IGRT), Adaptive radiation therapy (ART) has become a routine clinical practice on linear accelerators for the increase the tumor dose conformity and improvement of normal tissue sparing at the same time. For these highly developed techniques, megavoltage cone beam computed tomography (MVCBCT) system produce volumetric images at just one rotation of the x-ray beam source and detector on the bottom of conventional linear accelerator for real-time application of patient condition into treatment planning. MV CBCT image scan be directly registered to a reference CT data set which is usually kilo-voltage fan-beam computed tomography (kVFBCT) on treatment planning system and the registered image scan be used to adjust patient set-up error. However, to use MV CBCT images in radiotherapy, reliable electron density (ED) distribution are required. Patients scattering, beam hardening and softening effect caused by different energy application between kVCT, MV CBCT can cause cupping artifacts in MV CBCT images and distortion of Houns field Unit (HU) to ED conversion. The goal of this study, for reliable application of MV CBCT images into dose calculation, MV CBCT images was modified to correct distortion of HU to ED using the relationship of HU and ED from kV FBCT and MV CBCT images. The HU-density conversion was performed on MV CBCT image set using Dose difference map was showing in Figure 1. Finally, percentage differences above 3% were reduced depending on applying density calibration method. As a result, total error co uld be reduced to under 3%. The present study demonstrates that dose calculation accuracy using MV CBCT image set can be improved my applying HU-density conversion method. The dose calculation and comparison of dose distribution from MV CBCT image set with/without HU-density conversion method was performed. An advantage of this study compared to other approaches is that HU

  15. Modernization Transition of the Connotation and Practice Method of the Spirit of Lei Feng%论雷锋精神内涵及实践方式的现代转向

    Institute of Scientific and Technical Information of China (English)

    谢俊春

    2014-01-01

    Since the Chinese economic reform, our country’s political task and social practice environment have big differences with the past. In this situation, we must implement the modernization transition of the meaning and practice method of the spirit of Lei Feng, to advance and promote the civism, the innovation spirit, the independence spirit, and the team spirit, which are contained in the spirit of Lei Feng. Besides, the randomized temporary assistance should be converted to the formal social relief, and the personal behaviors should be changed into organized group behaviors. A variety of volunteer activities should be accumulated to rescue the huge weak community, so as to face the big challenge of the aging society.%改革开放以来,我国面临的政治社会任务和社会实践环境与过去已有很大的不同,要求我们必须实现雷锋精神内涵及实践方式的现代转向,着力提倡和弘扬雷锋精神蕴含的公民精神、创新精神、自主精神、团结精神,从强烈的阶级情感转向普遍的对全体社会成员乃至全人类的人文关怀,从计划经济年代的服从意识和臣民人格转向市场经济条件下的独立意识和公民人格。同时,由随机的临时的救助转向正规的社会工作救助,由分散的个人行为转向有组织的团体行为,发起各类志愿者行动,救助庞大的弱势群体,应对老龄社会提出的巨大挑战。

  16. Detrimental ELAVL-1/HuR-dependent GSK3β mRNA stabilization impairs resolution in acute respiratory distress syndrome

    Science.gov (United States)

    Hoffman, Olivia; Burns, Nana; Vadász, István; Eltzschig, Holger K.; Edwards, Michael G.

    2017-01-01

    A hallmark of acute respiratory distress syndrome (ARDS) is accumulation of protein-rich edema in the distal airspaces and its removal is critical for patient survival. Previous studies have shown a detrimental role of Glycogen Synthase Kinase (GSK) 3β during ARDS via inhibition of alveolar epithelial protein transport. We hypothesized that post-transcriptional regulation of GSK3β could play a functional role in ARDS resolution. To address this hypothesis, we performed an in silico analysis to identify regulatory genes whose expression correlation to GSK3β messenger RNA utilizing two lung cancer cell line array datasets. Among potential regulatory partners of GSK3β, these studies identified the RNA-binding protein ELAVL-1/HuR (Embryonic Lethal, Abnormal Vision, Drosophila-Like) as a central component in a likely GSK3β signaling network. ELAVL-1/HuR is a RNA-binding protein that selectively binds to AU-rich elements of mRNA and enhances its stability thereby increasing target gene expression. Subsequent studies with siRNA suppression of ELAVL-1/HuR demonstrated deceased GSK3β mRNA and protein expression and improved clearance of FITC-albumin in A549 cells. Conversely, stabilization of ELAVL-1/HuR with the proteasome inhibitor MG-132 resulted in induction of GSK3β at mRNA and protein level and attenuated FITC-albumin clearance. Utilizing ventilator-induced lung injury or intra-tracheal installation of hydrochloric acid to induce ARDS in mice, we observed increased mRNA and protein expression of ELAVL-1/HuR and GSK3β. Together, our findings indicate a previously unknown interaction between GSK3β and ELAV-1 during ARDS, and suggest the inhibition of the ELAV-1- GSK3β pathways as a novel ARDS treatment approach. PMID:28196122

  17. The Anti-Inflammatory Effect and Intestinal Barrier Protection of HU210 Differentially Depend on TLR4 Signaling in Dextran Sulfate Sodium-Induced Murine Colitis.

    Science.gov (United States)

    Lin, Sisi; Li, Yongyu; Shen, Li; Zhang, Ruiqin; Yang, Lizhi; Li, Min; Li, Kun; Fichna, Jakub

    2017-02-01

    Ulcerative colitis (UC) is strongly associated with inflammation and intestinal barrier disorder. The nonselective cannabinoid receptor agonist HU210 has been shown to ameliorate inflamed colon in colitis, but its effects on intestinal barrier function and extraintestinal inflammation are unclear. To investigate the effects and the underlying mechanism of HU210 action on the UC in relation to a role of TLR4 and MAP kinase signaling. Wild-type (WT) and TLR4 knockout (Tlr4 (-/-)) mice were exposed to 4% dextran sulfate sodium (DSS) for 7 days. The effects of HU210 on inflammation and intestinal barrier were explored. Upon DSS challenge, mice suffered from bloody stool, colon shortening, intestinal mucosa edema, pro-inflammatory cytokine increase and intestinal barrier destruction with goblet cell depletion, increased intestinal microflora accompanied with elevated plasma lipopolysaccharide, reduced mRNA expression of the intestinal tight junction proteins, and abnormal ratio of CD4(+)/CD8(+) T cells in the intestinal Peyer's patches. Pro-inflammatory cytokines in the plasma and the lung, as well as pulmonary myeloperoxidase activity, indicators of extraintestinal inflammation were increased. Protein expression of p38α and pp38 was up-regulated in the colon of WT mice. Tlr4 (-/-) mice showed milder colitis. HU210 reversed the intestinal barrier changes in both strains of mice, but alleviated inflammation only in WT mice. Our study indicates that in experimental colitis, HU210 displays a protective effect on the intestinal barrier function independently of the TLR4 signaling pathway; however, in the extraintestinal tissues, the anti-inflammatory action seems through affecting TLR4-mediated p38 mitogen-activated protein kinase pathway.

  18. Cytoplasmic expression of HuR may be a valuable diagnostic tool for determining the potential for malignant transformation of oral verrucous borderline lesions

    Science.gov (United States)

    HABIBA, UMMA; KITAMURA, TETSUYA; YANAGAWA-MATSUDA, AYA; HIDA, KYOKO; HIGASHINO, FUMIHIRO; OHIRO, YOICHI; TOTSUKA, YASUNORI; SHINDOH, MASANOBU

    2014-01-01

    Oral verrucous carcinoma (OVC) is a low grade variant of oral squamous cell carcinoma, and oral verrucous hyperplasia (OVH) is a benign lesion without malignant features. However, pathologists are sometimes presented with borderline lesions and are indecisive as to diagnose them as benign or malignant. Thus, these lesions are tentatively termed oral verrucous lesions (OVLs). HuR is an ARE mRNA-binding protein, normally localized in the nucleus but cytoplasmic exportation is frequently observed in cancer cells. The present study aimed to elucidate whether expression of the HuR protein facilitates the diagnosis of true malignant lesions. Clinicopathological features were evaluated, and immunohistochemical analysis for p53, Ki67 and HuR proteins was performed in 48 cases of OVH, OVC and OVL, and the outcomes were correlated using appropriate statistical analysis. The association of these three proteins in relation to malignant transformation was analyzed after a 3-year follow-up of 25 OVL cases. The basal characteristics (age, gender and location) of all cases had no significant association with the types of lesions. Gingiva (39.4%) was the common site for all lesions. Distribution of the examined proteins had a significant association with the lesions. As compared with the OVLs, the number of immunostained-positive cells was significantly higher in the OVCs and lower in the OVH cases. During follow-up, 24% of the OVLs underwent malignant transformation for which high HuR expression and a diffuse staining pattern in the epithelium were observed. Taken together, the high degree of HuR expression with diffuse staining pattern in the epithelium may be an effective diagnostic tool that determines the potential of OVLs for malignant transformation. PMID:24534848

  19. Cytoplasmic expression of HuR may be a valuable diagnostic tool for determining the potential for malignant transformation of oral verrucous borderline lesions.

    Science.gov (United States)

    Habiba, Umma; Kitamura, Tetsuya; Yanagawa-Matsuda, Aya; Hida, Kyoko; Higashino, Fumihiro; Ohiro, Yoichi; Totsuka, Yasunori; Shindoh, Masanobu

    2014-04-01

    Oral verrucous carcinoma (OVC) is a low grade variant of oral squamous cell carcinoma, and oral verrucous hyperplasia (OVH) is a benign lesion without malignant features. However, pathologists are sometimes presented with borderline lesions and are indecisive as to diagnose them as benign or malignant. Thus, these lesions are tentatively termed oral verrucous lesions (OVLs). HuR is an ARE mRNA-binding protein, normally localized in the nucleus but cytoplasmic exportation is frequently observed in cancer cells. The present study aimed to elucidate whether expression of the HuR protein facilitates the diagnosis of true malignant lesions. Clinicopathological features were evaluated, and immunohistochemical analysis for p53, Ki67 and HuR proteins was performed in 48 cases of OVH, OVC and OVL, and the outcomes were correlated using appropriate statistical analysis. The association of these three proteins in relation to malignant transformation was analyzed after a 3-year follow-up of 25 OVL cases. The basal characteristics (age, gender and location) of all cases had no significant association with the types of lesions. Gingiva (39.4%) was the common site for all lesions. Distribution of the examined proteins had a significant association with the lesions. As compared with the OVLs, the number of immunostained-positive cells was significantly higher in the OVCs and lower in the OVH cases. During follow-up, 24% of the OVLs underwent malignant transformation for which high HuR expression and a diffuse staining pattern in the epithelium were observed. Taken together, the high degree of HuR expression with diffuse staining pattern in the epithelium may be an effective diagnostic tool that determines the potential of OVLs for malignant transformation.

  20. Interleukin 1β Regulation of the System xc- Substrate-specific Subunit, xCT, in Primary Mouse Astrocytes Involves the RNA-binding Protein HuR.

    Science.gov (United States)

    Shi, Jingxue; He, Yan; Hewett, Sandra J; Hewett, James A

    2016-01-22

    System xc(-) is a heteromeric amino acid cystine/glutamate antiporter that is constitutively expressed by cells of the CNS, where it functions in the maintenance of intracellular glutathione and extracellular glutamate levels. We recently determined that the cytokine, IL-1β, increases the activity of system xc(-) in CNS astrocytes secondary to an up-regulation of its substrate-specific light chain, xCT, and that this occurs, in part, at the level of transcription. However, an in silico analysis of the murine xCT 3'-UTR identified numerous copies of adenine- and uridine-rich elements, raising the possibility that undefined trans-acting factors governing mRNA stability and translation may also contribute to xCT expression. Here we show that IL-1β increases the level of mRNA encoding xCT in primary cultures of astrocytes isolated from mouse cortex in association with an increase in xCT mRNA half-life. Additionally, IL-1β induces HuR translocation from the nucleus to the cytoplasm. RNA immunoprecipitation analysis reveals that HuR binds directly to the 3'-UTR of xCT in an IL-1β-dependent manner. Knockdown of endogenous HuR protein abrogates the IL-1β-mediated increase in xCT mRNA half-life, whereas overexpression of HuR in unstimulated primary mouse astrocytes doubles the half-life of constitutive xCT mRNA. This latter effect is accompanied by an increase in xCT protein levels, as well as a functional increase in system xc(-) activity. Altogether, these data support a critical role for HuR in mediating the IL-1β-induced stabilization of astrocyte xCT mRNA.

  1. Type-Ⅰcannabinoid receptor mediates down-regulation of CXCR4 in astrocytes by cannabinoid HU210%Ⅰ型大麻素受体介导大麻素HU210对星型胶质细胞CXCR4水平下调

    Institute of Scientific and Technical Information of China (English)

    朱舟; 韩静; 张遐; 徐逸

    2016-01-01

    目的 探寻大麻素抑制中枢神经系统免疫反应的机制,为大麻素临床药物的合理应用提供实验依据.方法 用不同浓度的HU210刺激培养的星形胶质细胞,利用Western blot检测并比较刺激组和未刺激组细胞CXCR4蛋白水平的差异,进而用Ⅰ型大麻素受体(type-1 cannabinoid receptor,CB1R)拮抗剂AM281刺激细胞后,观察HU210对CXCR4表达的影响.结果 Western blot检测结果显示,高浓度HU210能下调星形胶质细胞CXCR4表达,AM28能阻断HU210所致的CXCR4下调.结论 HU210经由CB1R下调CXCR4,这可能是大麻发挥免疫抑制作用的机理之一.

  2. 北宋蜀人冯山家世行年及《安岳集》版本考并补冯山佚诗%A Research on Feng Shan’ s Family, Biography,Version of Anyue Poetry and One Uncollected Poem

    Institute of Scientific and Technical Information of China (English)

    李清华

    2014-01-01

    冯山,北宋中期蜀中诗人,颇有盛名。其家世个人资料匮乏,今无系统研究。根据古籍相关记载,对其家世谱系略作整理,编次其个人行年及其创作,试图系统地研究冯山。对《安岳集》的版本作详细地考证论述,以期对四川文学研究略有补益。又据《永乐大典》补冯山《黄沙村》七律一首。%Feng Shan was a famous poet in Sichuan in the Song Dynasty. Because of the deficiency of historical re-cords, there is not a systematic study of him. According to the records of ancient books, the essay mainly gives a systematically textual research on Feng Shan,such as his family and biography,the version of Anyue Poetry,hoping to be of help for the literature study in Sichuan. Besides,the outhor finds one of Feng Shan’ poems uncollected in other existing books from The Yongle Canon.

  3. Re-exploration on the Origin of the Prehistoric Witch Religion of the Chinese Feng Shui%中国风水的史前巫教源头再探

    Institute of Scientific and Technical Information of China (English)

    胡义成

    2015-01-01

    As early as in the 20th century, the foreign archaeology and other disciplines revealed and proved that there existed the“bottom layer of the Arminius witch”.The theory of“bottom layer of the Arminius witch” retold by Mr.Zhang Guangzhi and it was mainly constructed by Mr.Frost, in which Frost expounded that the Arminius which possessed eight ideological characteristics. Mr.Zhang himself once said that witch was very important to the prehistoric China and the eight ideological characteristics of the Arminius which were quite suitable for the study of the ancient China.The above-mentioned ideas reflected that the study of prehis-toric witch had something to do with the study of origin of the Chinese Feng Shui.The article 2 of the eight ideological characteristics of the Arminius which said that there existed the gods of four directions and the gods of four quadrants;the cosmos was divided into four quadrants which were Sep.arated by central axis between south and north, and east and west;meanwhile every direction often combined with specific color;the article 4 said witches were helped by the animal gods.By careful comparison, we found that the a-bove-mentioned ideas accorded completely with the theory of the Chinese Feng Shui, in which there existed front southern god ( scarlet bird) , behind northern god ( tortoise) , left eastern god ( green dragon) , right western god ( white tiger);there was a bas-ic chart of the central axis of south and north.Article 5 said all phenomenon in the cosmos were endowed vigor by vitality or soul, which accorded with vigor philosophy proposed by the Chinese Feng Shui.All these evidences proved that the witch religion was the origin of the prehistoric culture of the Chinese Feng Shui.%国外考古学和其他学科早在20世纪就揭示并多学科证明了史前存在“亚美巫教底层”。张光直先生转述的“亚美巫教底层”理论主要建构者佛斯特先生关于其意识形态八

  4. “助人为乐”的德性论阐释:以雷锋精神为例%“Happiness in Helping Others”:An Explanation from the Theory of Virtue, with Example of Lei Feng Spirit

    Institute of Scientific and Technical Information of China (English)

    孙海霞

    2013-01-01

    助人为乐是雷锋精神的重要组成部分,在德性论的阐释视域中,雷锋助人为乐的精神展现出深刻的价值内涵和时代意义:它崇尚助人之“乐”,在德性完善中实现精神愉悦;在“乐”于助人的行为实践中,彰显自觉自主的道德精神境界;在助人“乐”己的人生信条中,绽放“人我合一”的“他者”情怀。%Helping others is a key part of Lei Feng Spirit. In the horizon of Virtue Theory, the Spirit presents profound values and contemporary significance: it highlights happiness and the spiritual enjoyment in virtue improvement. Its emphasis of the “will” in helping practice tells self-aware morality perfection; its principle of spiritual gains through helping implies an integration of self and others.

  5. 《孔子诗论》与荀子论《风》《雅》《颂》%Confucian Poetics and Xunzi’ s Criticism on Feng, Ya and Song

    Institute of Scientific and Technical Information of China (English)

    赵东栓

    2016-01-01

    Confucian Poetics, based on Confucius teaching The Book of Songs and scholars’ interpretation afterwards, basically reflects Confucian thoughts about The Book of Songs. This is overtly and covertly linked to criticism of The Book of Songs by Xunzi, who was the last master of Confucianism in the Pre-Qin period. Seen from Confucian Poetics and Xunzi’ s criticism on Feng, Ya and Song, there are some similar opinions between them, which shows that the relation between Confucian Poetics and Xunzi’ s criticism on The Book of Songs is an academic one of source and flow.%《孔子诗论》是孔子诗教及后学阐释的产物,基本上体现的是孔子的诗学思想,而先秦最后的儒学大师荀子的论《诗》,与之存在着一些显性或隐性的联系。从《孔子诗论》与荀子论《风》《雅》《颂》来看,二者存在着某些相同或相近的观点,这说明《孔子诗论》与荀子论《诗》存在着源和流的学术联系。

  6. The Discourse Cohesion Features of Modern Poem From "Sonnet Collection" of Feng Zhi%从冯至“十四行诗”的篇章衔接看诗歌篇章的特色

    Institute of Scientific and Technical Information of China (English)

    罗菲

    2012-01-01

    There was very little study for discourse cohesion of modern poems, and almost none for the comp,-u-ative study between discourse cohesion of poem and prose and other literature styles. It is not appropriate for the name of poem-country. In order to disclose discourse cohesion features and regular patterns of modern poem, "Sonnet Collec- tion" of Feng Zhi, a famous modern poet, has been analyzed, in the form of granmmtical, lexical, rhetorical and punctuation marks.%前人对现代诗歌的篇章衔接研究很少,涉及诗歌篇章衔接与散文等其他文体比较研究的则几乎没有,认为这与素有“诗国”之称的我国很不相称。因此,选取了现代著名诗人冯至的《十四行集》,从语法形式、词汇、修辞和标点符号四个方面来深入分析冯至诗歌的篇章结构衔接方式,以便更好地理解与探求现代诗歌的篇章衔接特色,并寻求其规律。

  7. Modelo de gestión cultural: Una experiencia con el Qhapaq Ñan en Huánuco

    Directory of Open Access Journals (Sweden)

    Denesy Palacios Jiménez

    2014-06-01

    Full Text Available Objetivos: Plantear el aprovechamiento del patrimonio cultural arqueológico del Qhapaq Ñan en Huánuco en condición de parque cultural, mediante un modelo eficiente de gestión cultural basada en la investigación, protección, conservación y uso social, para convertir a este lugar en un destino turístico y así contribuir con un proyecto no solo para salvaguardarlo sino también para incrementar los ingresos económicos y reducir los niveles de pobreza en la zona. Métodos: Se trata de un estudio que describe y recoge experiencias de gestión cultural respecto a cómo se aprovecha en otros sitios el legado o patrimonio cultural arqueológico, tanto en el ámbito mundial como nacional. La investigación fue cuantitativa y cualitativa, con un alcance correlacional. Se usó el diseño descriptivo, comparativo, analítico, histórico, deductivo. Resultados: El estudio plantea la necesidad de optimizar los recursos para el aprovechamiento del Qhapaq Ñan como parque cultural a través de una adecuada gestión cultural, y que dicho parque comprenderá 68,34 km, desde el tambo de Tambococha o Tunsucancha hasta el tambo de Taparako, por ser uno de los tramos mejor conservados e involucra importantes zonas de patrimonio cultural material e inmaterial. Conclusiones: El Camino Inca en Huánuco es uno de los patrimonios más importantes del país, y es posible ser impulsado como un parque cultural mediante un modelo de gestión cultural, si tenemos en cuenta las diversas experiencias de gestión en el país y en mundo, cuyos aportes deben servir de apoyo para un modelo de gestión en este campo.

  8. An Imagination and Discipline of Culture Model: The Narration and Diffusion of the Story about Feng Yan in the Song Dynasty%文化合模的想象与规训——冯燕故事在宋代的叙述与传播

    Institute of Scientific and Technical Information of China (English)

    张劲松

    2012-01-01

    Feng Yan is a roaming swordsman in The Legendary of Feng Yah in the Tang Dynasty. Feng com- mitted adultery with a woman. Later, he killed the woman and surrendered himself to the officials. He became a hero and was highly praised by the officials. However, his story was not popularly read and diffused. As a re- sult, he was not widely known in history at first. To the Song Dynasty, his story was integrated into the cultural model of the Song Dynasty as a sort of cultural spirit, talked about and thought about more widely than before. Zhang Qixian and Shangguan Rong rebuilt Feng~ story indirectly by writing notes and fictionally imagining. Zeng Bu sang directly about Feng Yan~ Chivalrous spirit in the form of ci ( a form of Chinese poem). In the Song Dy- nasty, Feng's story was not only praised on and on, but also given a new name of "being loyal to the emperor". His spirit was regarded as a model that would defend traditional Confucian morals.%冯燕是唐传奇《冯燕传》中的侠客,通人之妻而又杀之并自首官府。其侠义为士大夫所激赏歌颂。但历来研究其传播接受者较少。至宋代,冯燕故事犹如一种文化精神的母题融入宋人文化的模式中被重新叙述和想象。张齐贤和上官融以笔记记录和虚构想象的话语间接传释了冯燕故事。曾布以词直接歌咏了冯燕的侠义精神。冯燕故事在宋代不仅被继续歌颂,而且还被赋予了“忠君”报国的新义。冯燕的游侠气质被转化为维护儒家道德的理想楷模。

  9. Comparison between Feng-shui and Landscape Ecology Benefit of the Buddhist Temple Garden of Tiantai Stream:A Case Study of the Guangshan Jingju Temple and Tiantai Guoqing Temple%天台宗佛教寺庙环境中“风水”和景观生态效应的比较分析——以光山净居寺和天台国清寺为例

    Institute of Scientific and Technical Information of China (English)

    刘芳; 沈朝栋; 柴明良

    2012-01-01

    风水理论,作为我国传统文化的一部分,曾在民间广泛应用.该文在梳理风水理论及理想风水模式的基础上,以光山净居寺和天台国清寺为例,立足景观生态学相关理论对天台宗佛教寺庙环境中“风水”和景观生态效应进行了相关研究和分析,并总结出传统风水理论和景观生态效应之间的联系.%Feng-shui theory can be seen as a part of folklore and culture in China. The key value orientation of geomantic thought is that the dwelling space of human should be harmonious with the environment. Through analyzing the Feng-shui theory and idealized model, with the example of Jingju temple and Guoqing temple, this article analyzed and discussed the Feng-shui and landscape ecology benefit of the buddhist temple garden of Tiantai Stream based on landscape ecology, and summed up the connection between Feng-shui theory and landscape ecology benefit.

  10. Hepatitis B Virus X Upregulates HuR Protein Level to Stabilize HER2 Expression in Hepatocellular Carcinoma Cells

    Directory of Open Access Journals (Sweden)

    Chao-Ming Hung

    2014-01-01

    Full Text Available Hepatitis B virus- (HBV- associated hepatocellular carcinoma (HCC is the most common type of liver cancer. However, the underlying mechanism of HCC tumorigenesis is very complicated and HBV-encoded X protein (HBx has been reported to play the most important role in this process. Activation of downstream signal pathways of epidermal growth factor receptor (EGFR family is known to mediate HBx-dependent HCC tumor progression. Interestingly, HER2 (also known as ErbB2/Neu/EGFR2 is frequently overexpressed in HBx-expressing HCC patients and is associated with their poor prognosis. However, it remains unclear whether and how HBx regulates HER2 expression. In this study, our data showed that HBx expression increased HER2 protein level via enhancing its mRNA stability. The induction of RNA-binding protein HuR expression by HBx mediated the HER2 mRNA stabilization. Finally, the upregulated HER2 expression promoted the migration ability of HBx-expressing HCC cells. These findings deciphered the molecular mechanism of HBx-mediated HER2 upregulation in HBV-associated HCC.

  11. New Light-Travel Time Models and Orbital Stability Study of the Proposed Planetary System HU Aquarii

    CERN Document Server

    Hinse, Tobias C; Gozdziewski, Krzysztof; Haghighipour, Nader; Lee, Chung-Uk; Scullion, Eamon M

    2011-01-01

    In this work we propose a new orbital architecture for the two proposed circumbinary planets around the polar eclipsing binary HU Aquarii. We base the new two-planet, light-travel time model on the result of a Monte Carlo simulation driving a least-squares Levenberg-Marquardt minimisation algorithm on the observed eclipse egress times. Our best-fitting model with $\\chi_{r}^2=1.43$ resulted in high final eccentricities for the two companions leading to an unstable orbital configuration. From a large ensemble of initial guesses we examined the distribution of final eccentricities and semi-major axes for different $\\chi_{r}^2$ parameter intervals and encountered qualitatively a second population of best-fitting parameters. The main characteristic of this population is described by low-eccentric orbits favouring long-term orbital stability of the system. We present our best-fitting model candidate for the proposed two-planet system and demonstrate orbital stability over one million years using numerical integrati...

  12. Characterization and hepatoprotective effect of polysaccharides from Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou sarcocarp.

    Science.gov (United States)

    Yue, Yuan; Wu, Shuangchan; Zhang, Huifang; Zhang, Xuyan; Niu, Yunhui; Cao, Xiaoqin; Huang, Fuwei; Ding, Hong

    2014-12-01

    The polysaccharides from Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou sarcocarp (PWJS) is evaluated for the chemical composition, antioxidant activity and hepatoprotective effect. The characteristics of PWJS were determined by FT-IR spectral and HPLC analysis. The antioxidant activity was investigated using in vitro systems. An in vivo study of PWJS against CCl4 induced liver injury was also conducted. HPLC analysis showed that PWJS is an acidic heteropolysaccharide, rich in glucose (38.59%), arabinose (23.16%), galacturonic acid (17.64%) and galactose (10.44%). PWJS displayed strong antioxidant activity in vitro. In the in vivo study, PWJS treatment lowered the serum levels of ALT and AST in CCl4-intoxicated mice. Additionally, levels of antioxidant enzymes (SOD and CAT) and GSH were elevated in liver damage mice by PWJS intervention, while content of MDA was lessened. Meanwhile, PWJS reverses the suppression of Nrf2 nuclear translocation, and increases the protein expression of HO-1, GSTα and NQO1 in liver damage mice. Hematoxylin-eosin staining showed that the condition of liver damage was mitigated. This study demonstrates that PWJS reverses hepatotoxicity in CCl4-intoxicated mice through the mechanisms of antioxidant activity, as well as an augmentation of the Nrf2 pathway in liver tissue.

  13. A Network Pharmacology Approach to Uncover the Pharmacological Mechanism of XuanHuSuo Powder on Osteoarthritis

    Science.gov (United States)

    Zhang, Xinyue; Luo, Shilin; Zhang, Baixia; Duan, Xiaojie; Zhang, Zhiqian; Wang, Wenqi; Wang, Yun; Sun, Yikun

    2016-01-01

    As the most familiar type of arthritis and a chronic illness of the joints, Osteoarthritis (OA) affects a great number of people on the global scale. XuanHuSuo powder (XHSP), a conventional herbal formula from China, has been extensively applied in OA treatment. Nonetheless, its pharmacological mechanism has not been completely expounded. In this research, a network pharmacology approach has been chosen to study the pharmacological mechanism of XHSP on OA, and the pharmacology networks were established based on the relationship between four herbs found in XHSP, compound targets, and OA targets. The pathway enrichment analysis revealed that the significant bioprocess networks of XHSP on OA were regulation of inflammation, interleukin-1β (IL-1β) production and nitric oxide (NO) biosynthetic process, response to cytokine or estrogen stimuli, and antiapoptosis. These effects have not been reported previously. The comprehensive network pharmacology approach developed by our research has revealed, for the first time, a connection between four herbs found in XHSP, corresponding compound targets, and OA pathway systems that are conducive to expanding the clinical application of XHSP. The proposed network pharmacology approach could be a promising complementary method by which researchers might better evaluate multitarget or multicomponent drugs on a systematic level. PMID:27110264

  14. Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

    Directory of Open Access Journals (Sweden)

    Chun-Ching Lin

    2012-01-01

    Full Text Available Xiao-Chai-Hu-Tang (XCHT, a traditional Chinese medicine formula consisting of seven medicinal plants, is used in the treatment of various diseases. We show here that XCHT could protect type-1 diabetic mice against diabetic nephropathy, using streptozotocin (STZ-induced diabetic mice and high-glucose (HG-exposed rat mesangial cell (RMC as models. Following 4 weeks of oral administration with XCHT, renal functions and renal hypertrophy significantly improved in the STZ-diabetic mice, while serum glucose was only moderately reduced compared to vehicle treatment. Treatment with XCHT in the STZ-diabetic mice and HG-exposed RMC resulted in a decrease in expression levels of TGF-β1, fibronectin, and collagen IV, with concomitant increase in BMP-7 expression. Data from DPPH assay, DHE stain, and CM-H2DCFDA analysis indicated that XCHT could scavenge free radicals and inhibit high-glucose-induced ROS in RMCs. Taken together, these results suggest that treatment with XCHT can improve renal functions in STZ-diabetic mice, an effect that is potentially mediated through decreasing oxidative stress and production of TGF-β1, fibronectin, and collagen IV in the kidney during development of diabetic nephropathy. XCHT, therefore merits further investigation for application to improve renal functions in diabetic disorders.

  15. Synergistic Anticancer Effects of Vorinostat and Epigallocatechin-3-Gallate against HuCC-T1 Human Cholangiocarcinoma Cells

    Directory of Open Access Journals (Sweden)

    Tae Won Kwak

    2013-01-01

    Full Text Available The aim of this study was to investigate the effect of the combination of vorinostat and epigallocatechin-3-gallate against HuCC-T1 human cholangiocarcinoma cells. A novel chemotherapy strategy is required as cholangiocarcinomas rarely respond to conventional chemotherapeutic agents. Both vorinostat and EGCG induce apoptosis and suppress invasion, migration, and angiogenesis of tumor cells. The combination of vorinostat and EGCG showed synergistic growth inhibitory effects and induced apoptosis in tumor cells. The Bax/Bcl-2 expression ratio and caspase-3 and -7 activity increased, but poly (ADP-ribose polymerase expression decreased when compared to treatment with each agent alone. Furthermore, invasion, matrix metalloproteinase (MMP expression, and migration of tumor cells decreased following treatment with the vorinostat and EGCG combination compared to those of vorinostat or EGCG alone. Tube length and junction number of human umbilical vein endothelial cells (HUVECs decreased as well as vascular endothelial growth factor expression following vorinostat and EGCG combined treatment. These results indicate that the combination of vorinostat and EGCG had a synergistic effect on inhibiting tumor cell angiogenesis potential. We suggest that the combination of vorinostat and EGCG is a novel option for cholangiocarcinoma chemotherapy.

  16. Trigeminal Neuralgia as the First Clinical Manifestation of Anti-Hu Paraneoplastic Syndrome Induced by a Borderline Ovarian Mucinous Tumor

    Directory of Open Access Journals (Sweden)

    Hossein Kalanie

    2014-01-01

    Full Text Available Paraneoplastic neurologic syndrome (PNS is an uncommon manifestation of cancer that is not caused by the tumor or metastasis. Trigeminal neuralgia (TN is an initial symptom of this disease, but it has rarely been reported in the literature. Here, we report the case of a 76-year-old woman who presented with classic TN, followed by limbic encephalitis due to an underlying ovarian intestinal-type mucinous borderline tumor, with the presence of anti-Hu antibodies. She recovered quickly after removal of the tumor and was essentially free of symptoms 2 weeks after surgery. Because PNS precedes the tumor in approximately 60% of cases, its rapid detection and treatment are crucial. Therefore, we propose that PNS be considered during the management of TN when brain imaging is normal, as it is followed by other central and/or peripheral neurological manifestations as well as the presence of systemic symptoms such as anemia, fatigability, loss of appetite, or weight loss.

  17. Bioactive comparison of main components from unripe fruits of Rubus chingii Hu and identification of the effective component.

    Science.gov (United States)

    Zhang, Tian-Tian; Yang, Li; Jiang, Jian-Guo

    2015-07-01

    Dried and unripe fruit of Rubus chingii Hu, known as "Fu-pen-zi" in Chinese, has been used as a food and tonic in China for a long time. In order to analyze its effective ingredients, polysaccharides, flavonoids, saponins and alkaloids were extracted from the unripe fruits and their contents were determined. The in vitro antioxidant, anticomplementary and anticancer activities against human lung adenocarcinoma A549 cells of the four major constituents were investigated. Results showed that total flavonoids exhibited an obvious antioxidant activity, which was very close to ascorbic acid. The anticomplementary and anticancer activities of flavonoids were also the best among the four chemical compositions. Therefore, extraction process optimization of flavonoids was conducted using response surface methodology. The optimal conditions were as follows: extraction temperature 72.8 °C, ethanol concentration 30.67%, extraction time 2.66 h, and a liquid/solid ratio of 19.54 : 1. In addition, total flavonoids were subsequently separated by column chromatography and the major flavonoid was identified as tiliroside. Further experimental data revealed that tiliroside treatment could suppress the proliferation and induced the apoptosis of A549 cells.

  18. 胡绳史料学思想初探%Preliminary Study on Hu Sheng' s Thoughts of Historical Materials

    Institute of Scientific and Technical Information of China (English)

    胡现岭

    2012-01-01

    Hu Sheng was critical to the historians who were good at historical materials,arguing that their work was only the initial stage of historical research.This attitude didn' t mean that he ignored the important role of historical materials.He pointed out that we should guide historical materials by Marxism and historical data should service views;In addition,when carrying out the work of historical materials,we should estimate its value at the same time.%胡绳对以史料见长的史家一贯持批评态度,认为他们的工作只是停留在史学研究的初始阶段,但这并不表明他不重视史料在史学研究中的作用,而是提出以马克思主义来指导史料工作,在对史料进行考证的同时对其价值的高低做出评判,强调史料要为观点服务。

  19. Karyotypes of Akodon orophilus Osgood 1913 and Thomasomys sp. (Rodentia: Sigmodontinae from Huánuco, Peru

    Directory of Open Access Journals (Sweden)

    Víctor Pacheco

    2012-10-01

    Full Text Available Conventional chromosomal preparations were made of three native mice from Huánuco, Peru: a male and a female of Thomasomys sp., and a male of Akodon orophilus. Thomasomys sp. had a karyotype of 2n = 42, XY (n = 21, meanwhile A. orophilus presented 2n = 22, XY (n = 11. Comparisons between chromosomal pairs from the existent literature indicate that both are new karyotypes. Thomasomys sp. has a distinct sexual Y chromosome, the only metacentric (m reported for the genus. The chromosomes X and Y of A. orophilus are acrocentrics (a; and the length of chromosome Y (2/3 of the length of X distinguishes A. orophilus from other congeneric. Because the structural differences between the sexual chromosomes usually generates mechanism of reproductive isolation at intraspecific level and are bigger still in interspecific crosses, we concluded that the karyotypes reported here support the validity of the species A. orophilus and suggest that Thomasomys sp. represents a new species to science.

  20. Optimization of in vitro HBV replication and HBsAg production in HuH7 cell line.

    Science.gov (United States)

    Cavallone, Daniela; Moriconi, Francesco; Colombatto, Piero; Oliveri, Filippo; Bonino, Ferruccio; Brunetto, Maurizia Rossana

    2013-04-01

    The Gunther's vector-free method (GM), using PCR-amplified full length HBV-DNA (fl-HBV-DNA), is currently the best in vitro HBV replication system despite the low intracellular HBV-DNA production. The replication efficiency and HBsAg secretion of 12 isolates from HBsAg/HBeAg positive sera by GM, Monomer-Linear-Sticky-Ends-DNA (MLSE) and Monomer-Circular-Closed (MCC) were compared in HuH7 cells. Eight of twelve genomes (67%) were replication competent by GM; however direct sequencing (DS) showed that more than 80% of input DNA was undigested in spite of SapI treatment. Replication Intermediates (RI) were detected earlier (24 vs. 48h) and in higher amounts (2.51±0.32 and 6.43±0.43 fold) by MCC than GM or MLSE. By MCC 10 of 12 genomes (83%) were replication competent and 7 produced high RI levels. RI and HBsAg kinetics correlated positively in MCC (R=0.696, p=0.017 overall; R=0.928, p=0.008), but not in GM (R=-0.437, p=0.179 overall; R=-0.395, p=0.439) in genotype D isolates. In conclusion, HBV-DNA circularization prior transfection improves in vitro viral replication and replication competent HBsAg production, mimicking better the in vivo conditions.

  1. Avaliação dos óbitos ocorridos no Hospital Universitário da Universidade Federal de Santa Catarina (HU/UFSC Evaluation of deaths occurred at the University Hospital (HU/UFSC

    Directory of Open Access Journals (Sweden)

    Rachel Duarte Moritz

    2008-10-01

    Full Text Available OBJETIVO: Avaliar os óbitos ocorridos no HU/UFSC. Comparar o perfil dos pacientes que morreram nas enfermarias com o daqueles que morreram na UTI. Verificar se os óbitos foram considerados esperados (E e não evitáveis (NE. Constatar se houve recusa/suspensão de terapêutica (RST precedendo a morte. MÉTODOS: Obtivemos dados por meio de ficha elaborada pela Comissão de Óbito Hospitalar (COH, na qual constavam os aspectos demográficos, clínicos e terapêuticos dos pacientes e a avaliação da COH sobre a expectativa/evitabilidade dos óbitos. RESULTADOS: Analisamos os dados dos 326 pacientes com mais de 14 anos, que morreram entre julho/2004 e dezembro/2005. Desses, 128 morreram na UTI (G1 e 198 nas enfermarias (G2. A RST precedeu 38,2% dos óbitos no G1 e 2% no G2 (pOBJECTIVE: To evaluate the deaths which occurred at the HU/UFSC. To compare the profile of patients who died in the wards with that of patients who died in the ICU. To classify deaths which were expected or not, and avoidable or not. To verify how often withholding or withdrawing (WW therapy preceded death. METHODS: Archives of the Hospital Death Commission (HDC were analyzed. The clinical and demographic records were retrieved from the HDC data bank. Deaths were classified by the HDC as expected or not and avoidable or not. RESULTS: Data from 326 dead persons over 14 years of age were analyzed. One hundred and twenty eight deaths occurred in the ICU (G1 and 198 in the wards (G2. WW therapy preceded 38.2% of deaths in G1 and 2% in G2 (p<0.001. The main WW therapies were vasoactive drugs and antibiotics. Almost 20% of deaths were unexpected/avoidable in G1. In G2 6.5% were considered unexpected and 5% avoidable (p<0.005. Patients in G1 were younger and mostly male (p<0.005. Do-not-resuscitate orders were registered in 48.4% of patients' medical charts in G2 and 6.3% in G1 (p<0.001. Cardiopulmonary resuscitation was performed in 23.4% of patients in G1 and in 5.5% in G2 (p<0

  2. 22(R)-hydroxycholesterol induces HuR-dependent MAP kinase phosphatase-1 expression via mGluR5-mediated Ca(2+)/PKCα signaling.

    Science.gov (United States)

    Kim, Hyunmi; Woo, Joo Hong; Lee, Jee Hoon; Joe, Eun-Hye; Jou, Ilo

    2016-08-01

    MAP kinase phosphatase (MKP)-1 plays a pivotal role in controlling MAP kinase (MAPK)-dependent (patho) physiological processes. Although MKP-1 gene expression is tightly regulated at multiple levels, the underlying mechanistic details remain largely unknown. In this study, we demonstrate that MKP-1 expression is regulated at the post-transcriptional level by 22(R)-hydroxycholesterol [22(R)-HC] through a novel mechanism. 22(R)-HC induces Hu antigen R (HuR) phosphorylation, cytoplasmic translocation and binding to MKP-1 mRNA, resulting in stabilization of MKP-1 mRNA. The resulting increase in MKP-1 leads to suppression of JNK-mediated inflammatory responses in brain astrocytes. We further demonstrate that 22(R)-HC-induced phosphorylation of nuclear HuR is mediated by PKCα, which is activated in the cytosol by increases in intracellular Ca(2+) levels mediated by the phospholipase C/inositol 1,4,5-triphosphate receptor (PLC/IP3R) pathway and translocates from cytoplasm to nucleus. In addition, pharmacological interventions reveal that metabotropic glutamate receptor5 (mGluR5) is responsible for the increases in intracellular Ca(2+) that underlie these actions of 22(R)-HC. Collectively, our findings identify a novel anti-inflammatory mechanism of 22(R)-HC, which acts through PKCα-mediated cytoplasmic shuttling of HuR to post-transcriptionally regulate MKP-1 expression. These findings provide an experimental basis for the development of a RNA-targeted therapeutic agent to control MAPK-dependent inflammatory responses.

  3. Intratumoral hu14.18-IL-2 (IC) induces local and systemic antitumor effects that involve both activated T and NK cells as well as enhanced IC retention.

    Science.gov (United States)

    Yang, Richard K; Kalogriopoulos, Nicholas A; Rakhmilevich, Alexander L; Ranheim, Erik A; Seo, Songwon; Kim, Kyungmann; Alderson, Kory L; Gan, Jacek; Reisfeld, Ralph A; Gillies, Stephen D; Hank, Jacquelyn A; Sondel, Paul M

    2012-09-01

    hu14.18-IL-2 (IC) is an immunocytokine consisting of human IL-2 linked to hu14.18 mAb, which recognizes the GD2 disialoganglioside. Phase 2 clinical trials of i.v. hu14.18-IL-2 (i.v.-IC) in neuroblastoma and melanoma are underway and have already demonstrated activity in neuroblastoma. We showed previously that intratumoral hu14.18-IL-2 (IT-IC) results in enhanced antitumor activity in mouse models compared with i.v.-IC. The studies presented in this article were designed to determine the mechanisms involved in this enhanced activity and to support the future clinical testing of intratumoral administration of immunocytokines. Improved survival and inhibition of growth of both local and distant tumors were observed in A/J mice bearing s.c. NXS2 neuroblastomas treated with IT-IC compared with those treated with i.v.-IC or control mice. The local and systemic antitumor effects of IT-IC were inhibited by depletion of NK cells or T cells. IT-IC resulted in increased NKG2D receptors on intratumoral NKG2A/C/E⁺ NKp46⁺ NK cells and NKG2A/C/E⁺ CD8⁺ T cells compared with control mice or mice treated with i.v.-IC. NKG2D levels were augmented more in tumor-infiltrating lymphocytes compared with splenocytes, supporting the localized nature of the intratumoral changes induced by IT-IC treatment. Prolonged retention of IC at the tumor site was seen with IT-IC compared with i.v.-IC. Overall, IT-IC resulted in increased numbers of activated T and NK cells within tumors, better IC retention in the tumor, enhanced inhibition of tumor growth, and improved survival compared with i.v.-IC.

  4. A new species of frog of the genus Pristimantis from Tingo María National Park, Huánuco Department, central Peru (Anura, Craugastoridae)

    Science.gov (United States)

    Chávez, Germán; Catenazzi, Alessandro

    2016-01-01

    Abstract A new species of Craugastoridae frog encountered from 1000–1700 m in elevation in the premontane forests of the Peruvian central Andes is described. The new species is similar in appearance to many other species of Pristimantis, but is easily distinguishable from these species by having bright red coloration on the groin, posterior surface of thighs, and shanks. The new species is only known for two localities 27 km apart in the Huánuco Region. PMID:27587978

  5. Contents of the heavy metals nickel and lead in leaves of Paulownia elongata S.Y. Hu and Paulownia fortunei Hems. in Serbia

    OpenAIRE

    2009-01-01

    A comparative study was carried out on the capabilities of the two species Paulownia elongata S. Y. Hu and Paulownia fortunei Hems. as biomonitors of atmospheric (and soil) heavy-metal pollution. Research on content of the heavy metals nickel (Ni) and lead (Pb) in leaves of the species Paulownia elongata growing under urban and suburban conditions with the comparison to the concentration of these elements in leaves of the species Paulownia elongata and Paulownia fortunei on an experimental pl...

  6. La presencia de Ixodes luciae en el noroeste argentino y nuevos huéspedes para Ixodes pararicinus y algunas especies de Amblyomma (Acari: Ixodidae

    Directory of Open Access Journals (Sweden)

    Analía G. AUTINO

    2006-01-01

    Full Text Available El análisis de una colección de garrapatas de mamíferos del noroeste argentino, depositados en la Colección de Anexos de la Colección Mamíferos Lillo (CML de la Universidad Nacional de Tucumán, permitió ampliar el área de distribución de Ixodes luciae Sénevet, a las provincias de Salta y Tucumán e incrementar su rango de huéspedes al roedor sigmodontino Calomys callosus (Rengger y a los marsupiales de la familia Didelphidae Micoureus constantiae (Thomas, Thylamys cinderella Thomas y Thylamys venustus (Thomas. Se registró a C. callosus y Oligoryzomys destructor (Tschudi como nuevos huéspedes de Ixodes pararicinus Keirans & Clifford, y a los Carnivora Lycalopex gymnocercus (Fischer y Oncifelis geoffroyi (d ́Orbigny & Gervais como nuevos huéspedes de Amblyomma neumanni Ribaga y Amblyomma parvum Aragão, respectivamente. Adicionalmente, se observó la infestación de Lutreolina crassicaudata (Desmarest (Didelphidae por I. luciae y la presencia de Amblyomma tigrinum Koch, sobre L. gymnocercus. Ninfas de Amblyomma sp. se obtuvieron de Akodon Meyen (Sigmodontinae.

  7. Effects of r—HuEPO on the biophysical characteristics of erythrocyte membrane in patients with anemia of chronic renal failure

    Institute of Scientific and Technical Information of China (English)

    SHIHONGLIAN; FAJUNYANG; 等

    1994-01-01

    Using electron spin resonance (ESR) spin labeling technique,we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and mem brane fluidity of red blood cells(RBCs) from two groups of patients with anemia of chronic renal failure(ACRF).One of the groups is composed of patients who were untreated with recombinant human erythropoietin(r-HuEPO),and the other is composed of patients who were treated with r-HuEPO.The results indicated:1)the conformation of SH group binding site in RBC membrane proteins from former group was different from those of healty people.2)the fluidity in the region near the surface of RBC membrane from former group was lower than those of healthy people.3)However,the above biophysical properties of RBC membrane from later group were normal.We concluded that RBC membrane in patients with ACRF was abnormal,and the treatment of r-HuEPO may promote the production of normal RBCs,thus ameliorate the biophysical properties of RBCs from the patients with ACRF.

  8. HuR and Ago2 Bind the Internal Ribosome Entry Site of Enterovirus 71 and Promote Virus Translation and Replication.

    Directory of Open Access Journals (Sweden)

    Jing-Yi Lin

    Full Text Available EV71 (enterovirus 71 RNA contains an internal ribosomal entry site (IRES that directs cap-independent initiation of translation. IRES-dependent translation requires the host's translation initiation factors and IRES-associated trans-acting factors (ITAFs. We reported recently that mRNA decay factor AUF1 is a negative-acting ITAF that binds IRES stem-loop II. We also reported that the small RNA-processing enzyme Dicer produces at least four small RNAs (vsRNAs from the EV71 IRES. One of these, vsRNA1, derived from IRES stem-loop II, reduces IRES activity and virus replication. Since its mechanism of action is unknown, we hypothesized that it might control association of ITAFs with the IRES. Here, we identified the mRNA stability factor HuR and the RISC subunit Argonaute 2 (Ago2 as two ITAFs that bind stem-loop II. In contrast to AUF1, HuR and Ago2 promote EV71 IRES activity and virus replication. In vitro RNA-binding assays revealed that vsRNA1 can alter association of Ago2, HuR, and AUF1 with stem-loop II. This presents a possible mechanism by which vsRNA1 could control viral translation and replication.

  9. 论胡锦涛党建思想的基本价值%On the fundamental value of the Party building thought Hu Jintao

    Institute of Scientific and Technical Information of China (English)

    李强

    2011-01-01

    Since Sixteenth National Congress of the Communist Party of China,Hu Jintao as general secretary of the party leadership to actively promote the construction of the new great project,and achieved fruitful theoretical results,the formation of a building thought Hu.Hu building thought to enrich and develop Marxism in the ideological theory of party building treasury,for the party is always ahead of the times,firmly implement the Party's governing mission to provide a scientific theory and a powerful ideological weapon.%党的十六大以来,以胡锦涛为总书记的领导集体积极推进党的建设的伟大工程,取得了丰硕的理论成果,并形成了胡锦涛党建思想。胡锦涛党建思想丰富和发展了马克思主义党建理论的思想宝库,为使党始终走在时代前列,坚定不移实现党的执政使命,提供了科学理论和强大的思想武器。

  10. A comparative study of oral health attitudes and behavior using the Hiroshima University-Dental Behavioral Inventory (HU-DBI) between dental and civil engineering students in Colombia.

    Science.gov (United States)

    Jaramillo, Jorge A; Jaramillo, Fredy; Kador, Itzjak; Masuoka, David; Tong, Liyue; Ahn, Chul; Komabayashi, Takashi

    2013-03-01

    The aim of this study was to use the Hiroshima University - Dental Behavioral Inventory (HU-DBI) to compare oral health attitudes and behavior of dental and civil engineering students in Colombia. The HU-DBI's survey consisting of twenty dichotomous responses (agree-disagree) regarding tooth brushing, was completed at University Antonio Narino for the dental students and the University of Cauca for the civil engineering students. The Spanish version of the HU-DBI questionnaire was taken by 182 of 247 dental students and 411 of 762 engineering students. The data was-statistically analyzed by the chi-square test and backward logistic regression. Compared to the engineering students, the dental students were more likely to agree with questions such as "I am bothered by the color of my gums"(OR = 2.2, 95% CI: 1.3-3.7),"I think I can clean my teeth well without using toothpaste" (OR = 3.0, 95% CI: 1.5-5.9), "I have used a dye to see how clean my teeth are" (OR = 2.9, 95% CI: 1.9-4.3), and "I have had my dentist tell me that I brush very well" (OR = 2.0, 95% CI: 1.3-3.1). The dental education curriculum in a dental school compared to a civil engineering school in Colombia indicated that a three-phase curriculum in didactics and clinics increased oral health attitudes and behavior from entry to graduation.

  11. ESR1 Gene Polymorphisms and Prostate Cancer Risk: A HuGE Review and Meta-Analysis.

    Directory of Open Access Journals (Sweden)

    Yu-Mei Wang

    Full Text Available Many published data on the association between single nucleotide polymorphisms (SNPs in the ESR1 gene and prostate cancer susceptibility are inconclusive. The aim of this Human Genome Epidemiology (HuGE review and meta-analysis is to derive a more precise estimation of this relationship.A literature search of PubMed, Embase, Web of Science and Chinese Biomedical (CBM databases was conducted from their inception through July 1st, 2012. Crude odds ratios (ORs with 95% confidence intervals (CIs were calculated to assess the strength of association.Twelve case-control studies were included with a total 2,165 prostate cancer cases and 3,361 healthy controls. When all the eligible studies were pooled into the meta-analysis, ESR1 PvuII (C>T and XbaI (A>G polymorphisms showed no association with the risk of prostate cancer. However, in the stratified analyses based on ethnicity and country, the results indicated that ESR1 PvuII (C>T polymorphism was significantly associated with increased risk of prostate cancer among Asian populations, especially among Indian population; while ESR1 XbaI (A>G polymorphism may significantly increase the risk of prostate cancer among American population. Furthermore, we also performed a pooled analysis for all eligible case-control studies to explore the role of codon 10 (T>C, codon 325 (C>G, codon 594 (G>A and +261G>C polymorphisms in prostate cancer risk. Nevertheless, no significant associations between these polymorphisms and the risk of prostate cancer were observed.Results from the current meta-analysis indicate that ESR1 PvuII (C>T polymorphism may be a risk factor for prostate cancer among Asian populations, especially among Indian population; while ESR1 XbaI (A>G polymorphism may increase the risk of prostate cancer among American population.

  12. CYP3A4*1B polymorphism and cancer risk: a HuGE review and meta-analysis.

    Science.gov (United States)

    Zhou, Li-Ping; Yao, Fan; Luan, Hong; Wang, Yin-Ling; Dong, Xi-Hua; Zhou, Wen-Wen; Wang, Qi-Hui

    2013-04-01

    CYP450 3A4 (CYP3A4), encoded by the CYP3A4 gene, is a major enzyme catalyzing the metabolism of both endogenous and exogenous agents that may play a role in the etiology of carcinogenesis. Several potentially functional polymorphisms of the CYP3A4 gene have been implicated in cancer risk, but individually published studies have shown inconclusive results. The aim of this Human Genome Epidemiology (HuGE) review and meta-analysis was to investigate the association between CYP3A4*1B (rs2740574 A > G) polymorphism and cancer risk. Eleven studies were included with a total of 3,810 cancer patients and 3,173 healthy controls. We found that the G allele and GG genotype of CYP3A4*1B polymorphism were associated with increased risk of cancers using the fixed effects model (allele model: odds ratio (OR) = 1.24, 95 %CI: 1.09-1.42, P = 0.001; recessive model: OR = 1.77, 95 %CI: 1.30-2.41, P cancer type showed that the G allele and G carrier (AG + GG) of CYP3A4*1B polymorphism had significant associations with increased risk of prostate cancer, but not with breast cancer, leukemia, or other cancers. With further subgroup analysis based on different ethnicities, the results indicated that the GG genotype of CYP3A4*1B polymorphism might increase the risk of cancer among African populations. However, similar associations were not observed among Caucasian and Asian populations. Results from the current meta-analysis indicate that the G allele and GG genotype of CYP3A4*1B polymorphism might be associated with increased cancer risk, especially for prostate cancer among African populations.

  13. Bai-hu-tang, ancient chinese medicine formula, may provide a new complementary treatment option for sepsis.

    Science.gov (United States)

    Lin, Chien-Jung; Su, Yi-Chang; Lee, Cheng-Hung; Li, Tsai-Chung; Chen, Yun-An; Lin, Sunny Jui-Shan

    2013-01-01

    Bai-Hu-Tang (BHT) has been broadly applied to treating the early stage of acute infection with systemic inflammation for two thousand years in Chinese medicine. We explore whether BHT is beneficial in treating sepsis and its effects on proinflammatory cytokine, interleukin-6, and anti-inflammatory cytokine interleukin-10, in which both play key roles in the progress of sepsis. Thirty-six male Sprague-Dawley rats were randomized into six groups, with cecal ligation and puncture (CLP) performed in all but the sham-control group. Rats in CLP + BHT-L6 and CLP + BHT-H6 groups, respectively, received a low (0.45 g/kg) and high doses (0.9 g/kg) of BHT, 6 hrs postoperatively. CLP + BHT-L12 and CLP + BHT-H12 groups, respectively, received low and high doses of BHT, 12 hrs postoperatively. Sham-control and sepsis-control groups received distilled water (1 mL) as vehicle, 6 hrs postoperatively. Serial blood samples were drawn before operation, as baseline, and at 4, 8, and 12 hrs postoperatively for IL-6 and IL-10 assay. All rats were monitored for 3 days for survival study. Rats in the CLP + BHT-H6 group had significantly higher survival rate (80%) and significantly lower levels of both IL-6 and IL-10 at 12 hrs postoperatively than those in the sepsis-control group. Results suggested that BHT may be a new complementary treatment option for sepsis.

  14. Redescription and molecular analysis of Myxobolus shantungensis Hu, 1965 (Myxozoa: Myxosporea) infecting common carp Cyprinus carpio haematopterus.

    Science.gov (United States)

    Liu, Yang; Gu, Zemao; Zhang, Yunchao; Zeng, Lingbing

    2011-12-01

    Myxobolus shantungensis Hu, 1965 infects gill arches of common carp Cyprinus carpio haematopterus (Temminck and Schlegel), causing serious pathological effects on host fish. An inadequate original description and absence of molecular data make accurate early diagnosis challenging. To augment the original description, M. shantungensis is redescribed here using morphological and molecular biological methods. Mature spores of M. shantungensis were ellipsoidal or apple shaped in frontal view and lemon shaped in lateral view, averaging 8.2  ±  0.3 μm (8.0-9.0 μm) ×  10.1 ±  0.5 μm (9.2-11.1 μm)  ×  6.9  ±  0.3 μm (6.0-7.4 μm). Some spores had three to four "V"-shaped valve edge markings on the posterior of the spore. The two equal polar capsules were oval, measuring 4.3  ±  0.3 μm (3.8-5.0 μm)  ×  3.2  ±  0.2 μm (2.8-3.5 μm), situated at the anterior extremity of the spore. Polar filaments coiled with six to seven turns. Scanning electron microscopy revealed that the surface of mature spores of M. shantungensis was generally pitted with a number of irregular ridges in shape. M. shantungensis is also characterized on the molecular level using the sequence of the small subunit ribosomal RNA gene. A BLAST search revealed that this sequence did not match any available sequences in GenBank.

  15. Genetic polymorphisms of CASR and cancer risk: evidence from meta-analysis and HuGE review

    Directory of Open Access Journals (Sweden)

    Jeong S

    2016-02-01

    Full Text Available Sohyun Jeong, Jae Hyun Kim, Myeong Gyu Kim, Nayoung Han, In-Wha Kim, Therasa Kim, Jung Mi Oh College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, South Korea Background: CASR gene appears to be involved in cancer biology and physiology. However, a number of studies investigating CASR polymorphisms and cancer risks have presented inconclusive results. Thus, a systematic review and a meta-analysis of the effect of CASR polymorphisms on several cancer risks were performed to suggest a statistical evidence for the association of CASR polymorphisms with cancer risks.Methods: MEDLINE, EMBASE, Web of Science, Scopus, and the HuGE databases were searched. Nineteen articles of case–control and cohort studies were included for the final analysis.Results: The colorectal cancer risk was reduced in proximal (odds ratio [OR] =0.679, P=0.001 and distal (OR =0.753, P=0.026 colon sites with GG genotype of CASR rs1042636 and increased in distal colon site (OR =1.418, P=0.039 with GG genotype of rs1801726 by additive genetic model. The rs17251221 demonstrated noticeable associations that carrying a homozygote variant increases breast and prostate cancer risk considerably.Conclusion: The significant association of CASR polymorphisms with several cancer risks was observed in this review. In particular, the act of CASR polymorphisms as a tumor suppressor or an oncogene differs by cancer site and can be the research target for tumorigenesis. Keywords: rs1042636, rs1801725, rs1801726, systematic review, colorectal cancer

  16. 顺丰速运C2B电子商务网站设计初探%Exploration of Shun Feng Express' E-commerce Website Design On C2B

    Institute of Scientific and Technical Information of China (English)

    葛慧敏; 吴麟麟; 陈龙; 许人义

    2011-01-01

    Based on detailed analysis on value-added logistics service and C2B operational mode,we set Shun Feng Express as an example and then come up with a proposal about the value-added logistics services on C2B platform.This proposal includes expatiation on new-typed C2B concept;analytic data about market efficiency,actualities of e-commerce and costs of website design;risk assessment on Shun Feng's use of C2B website via fuzzy comprehensive evaluation method.Having colligated all the aspects mentioned,we finally prove our proposal has a great practicability.%在对物流增值服务及C2B模式进行分析的基础上,以顺丰速运为例,提出了基于C2B电子商务网站的物流增值服务的方案,并对新型C2B商务模型的概念进行了详细阐述。并通过对电子商务现状的分析、市场效益的分析、网站设计成本的分析以及运用模糊综合评价的方法对顺丰速运C2B电子商务网站的风险进行评估,验证了方案的切实可行性。

  17. Morphological Analysis on Dr. LAU Feng-shueh’s Neo-classical Dance%刘凤学“新古典”舞蹈文化形态分析

    Institute of Scientific and Technical Information of China (English)

    李超

    2015-01-01

    本文以台湾刘凤学“新古典舞团”舞蹈作品为例,对舞蹈艺术在当代文化语境下的“新古典”形态进行考察,并从训练方法、剧场演出形态、创作观等几个方面对刘凤学之“新古典”舞蹈观念进行文化形态分析研究。文章通过“文化的身体与技术的身体”两个层面的阐述对刘凤学唐乐舞训练之途径和目的展开探讨,从“古典精神的当代时态”之角度集中论述了刘凤学唐乐舞在形式及审美中与当下时空的融合及对抗,并通过对不同作品的创作分析注述了“新古典”在现代创作中的探索和实践。%This paper explores the Neo-classical dance of Lau feng-shueh ’s dance works existing in the contemporary,and analyzes the conception of Neo-classical dance from the perspectives of body training,morphology of theatre dance and the idea of choreography,explaining the significance and effect of Neo-classical dance in the contemporary choreography and performing practice.

  18. Human Trafficking in Feng Menglong and Ming Dynasty as Seen in Ling Mengchu' s Works%从“三言”“二拍”看明代人口贩卖现象

    Institute of Scientific and Technical Information of China (English)

    许菁频

    2011-01-01

    “三言”“二拍”中有关人口贩卖的篇目多达三四十篇,大致可分为亲人贩卖、主人发卖和人贩拐卖三类。人口贩卖既与“钱本位”下的生存压力有关,也与社会黑暗、盗贼猖獗相关。小说对人口贩卖的描写,使我们感受到了封建社会女性地位的低下、忠孝观念对人们思想的禁锢以及明末贞洁观念的渐趋淡薄。%There are thirty to forty stories in the three volumes of Words by Feng Menglong ( 1574 - 1646) and the two volumes of Slapping by Ling Mengchu (1580 -1644) that contain descriptions of human trafficking, falling into three categories: trafficking by families, by owners and by abductors. While the roaring business of human trafficking was attributable to the financial pressure in a money-worshipping society, it was closely related to the social injustice and prevalence of robbery and burglary at that time. From the descriptions in the novels we can infer the low status of women in the feudal society, the shackles of the so-called loyalty and filial piety on people' s mind, and the diminished concept of chastity during the end of the Ming dynasty ( 1368 - 1644).

  19. 论“三言”女性形象死亡叙事的审美意蕴%On Aesthetic Connotation of Death Narration of Female Images in FENG meng-long's Three Novel Collections

    Institute of Scientific and Technical Information of China (English)

    臧国书

    2012-01-01

    In his three collections of novels, FENG Meng-long combined the traditional concepts and time spirit and fe- male images were no longer the disaster sources and tools. When alive, they were real and had their own life stage. As for death, they lost their lives for their dreams hence they had their unique aesthetic features. The common narrative aesthetic fea- tures of positive figures are no hesitation to die for justice or a debt of gratitude and brave pursuit for love and self-respect. While the narrative aesthetic significance of the negative figures lies in that the malefactors, carnalists or degenerated deserved their punishment or flogging.%在“三言”中冯梦龙将传统观念与时代精神糅合塑造的女性形象不再是“祸水”和“工具”。生时,她们成为了实实在在的人,有鲜活的人生舞台;死时,她们为自己的理想和欲望而献身,具有独特的审美属性。正面形象,死亡叙事的审美共性是为正义、为报恩不惜一死的崇高;对爱、对尊严勇敢追逐的深情赞美。反面形象,死亡叙事的审美意味是作恶者、纵欲者的罪有应得;对丑、对堕落的无情鞭挞。

  20. The Utility of Human Plasma-Derived Butyrylcholinesterase (huBuChE) as a Therapeutic Measure in the Absence of Pre-Treatment or Conventional Post-Poisoning Therapies Against Nerve Agent

    Science.gov (United States)

    2011-10-01

    Human butyrylcholinesterase (huBuChE) has investigational new drug (IND) status in the U.S. as a pretreatment against organophosphate poisoning in humans...huBuChE) as a therapeutic measure in the absence of pre-treatment or conventional post- poisoning therapies against nerve agent. PRINCIPAL...absence of pre- treatment or conventional post- poisoning therapies against nerve agent. 5a. CONTRACT NUMBER W81WXH-10- -0044 5b. GRANT NUMBER 5c

  1. A.~()HU

    African Journals Online (AJOL)

    officer training college to improve the professional ... exercises'. ... in 1924 of the first Labour Government, headed by ... infantry tactics and stressed riding skills. At heart, ... sergeant and cadet Prince Henry, third son of .... 'Vorld, are led by men trained at Sandhurst (for stu- .... lurst does not need a 'system' to maintain its high.

  2. Cardiovascular effects of ethanol extract of Rubus chingii Hu (Rosaceae) in rats: an in vivo and in vitro approach.

    Science.gov (United States)

    Su, X H; Duan, R; Sun, Y Y; Wen, J F; Kang, D G; Lee, H S; Cho, K W; Jin, S N

    2014-06-01

    Rubus chingii Hu (Rosaceae) is an important traditional Chinese medicine that has been used to improve function of the kidney and treat excessive polyuria. However, the effects of Rubus chingii on the cardiovascular system and its pharmacological mechanisms of action have not been studied. The aim of the present study was to evaluate the cardiovascular effects of ethanol extract of Rubus chingii (ERC) in rats. The changes in systolic blood pressure and heart rate of rats and vascular tone of aortic rings in in vitro were measured using pressure transducer and force transducer, respectively, connected to a multichannel recording system. ERC decreased systolic blood pressure and heart rate in a concentration-dependent manner. ERC induced vasorelaxation in a concentration-dependent manner. The ERC-induced vasorelaxation was not observed in the absence of the endothelium. The vasorelaxant effect of ERC was significantly attenuated by inhibition of endothelial NO synthase (eNOS), soluble guanylyl cyclase (sGC), or Ca(2+) entry from extracellular sources with L-NAME, ODQ, diltiazem, or extracellular Ca(2+) depletion, respectively. Similarly, an inhibition of Akt with wortmannin attenuated the ERC-induced vasorelaxation. Modulators of the store-operated Ca(2+) entry, thapsigargin, Gd(3+), and 2-aminoethyl diphenylborinate markedly attenuated the ERC-induced vasorelaxation. Furthermore, 4-aminopyridine an inhibitor of voltage-dependent K(+) (KV) channel, significantly attenuated the ERC-induced vasorelaxation. However, tetraethylammonium and glibenclamide, had no significant effect on the ERC-induced vasorelaxation. Indomethacin, atropine, and propranolol had no effects on the ERC-induced vasorelaxation. The present study demonstrates that ERC induces vasorelaxation via endothelium-dependent two-step signaling: an activation of the Ca(2+)-eNOS-NO signaling in the endothelial cells and then subsequent stimulation of the NO-sGC-cGMP-KV channel signaling in the vascular

  3. A biblioterapia na humanização da assistência hospitalar do Hospital Universitário da Universidade Federal de Santa Catarina – HU/UFSC/Bibliotherapy for the hospital care humanization in the university hospital of Santa Catarina Federal University – HU/UFSC

    Directory of Open Access Journals (Sweden)

    Eva Seitz

    2008-01-01

    Full Text Available Este artigo tem por finalidade apresentar resultados do estudo realizado com pacientes, acompanhantes e funcionários das Unidades de Internação Cirúrgica do Hospital Universitário da Universidade Federal de Santa Catarina (UIC/HU/UFSC. O foco central foi o de verificar até que ponto a prática biblioterapêutica e as atividades de lazer contribuem no processo de Humanização da Assistência Hospitalar dos pacientes internados nas (UIC/HU/UFSC, na percepção dos pacientes, acompanhantes, funcionários da enfermagem e assistentes sociais da referida unidade de internação. Foram entrevistados 16 pacientes, 11 acompanhantes e 13 funcionários da enfermagem e assistentes sociais. As atividades de lazer analisadas neste estudo foram a prática biblioterapêutica, as atividades do grupo Reaja e a dança. Trata-se de uma pesquisa quantitativa e qualitativa. Quanto ao procedimento técnico, a presente pesquisa é classificada como estudo de caso, no qual, utilizou-se, para a coleta dos dados a entrevista estruturada e, cujos resultados demonstram que as atividades contribuem muito na Humanização da Assistência Hospitalar dos pacientes internados na UIC/HU/UFSC. This article has for purpose to present resulted of the study carried through with patients, companions and employees of the Units of Surgical Internment of the University Hospital of the Federal University of Santa Catarina (UIC/HU/UFSC. The central focus was to verify until point the practical library-therapy and the activities of leisure contribute in the process of Humanization of the Hospital Assistance of the patients interned in (UIC/HU/UFSC, in the perception of the patients, companions, employees of the nursing and social assistants of the related unit of internment. 16 patients, 11 companions and 13 social employees of the nursing and assistants had been interviewed. The analyzed activities of leisure in this study had been the practical library-therapy, the activities of

  4. Anti-Hu,Yo antibodies in patients with positive serum AQP4 antibody%水通道蛋白-4抗体阳性患者血清抗Hu、Yo抗体的检测

    Institute of Scientific and Technical Information of China (English)

    龙友明; 单福兰; 解龙昌; 陈梦宇; 郑杨波; 高聪; 杨宁

    2014-01-01

    Objective To evaluate the positive rates of serum anti‐Hu , and anti‐Yo antibodies in patients with positive serum aquaporin‐4 (AQP4) antibodies .Methods A consecutive cohort of 80 subjects were analyzed retrospectively . First , sera of patients were tested for AQP4 antibodies in an indirect immunofluorescence assay employing HEK‐293 cells transected with recombinant human AQP4 .Second ,anti‐Hu ,and anti‐Yo antibodies were tested with monkey cerebellum substrate by an indirect immunofluorescence assay .Results Eighty patients with positive AQP4 antibodies were confirmed in the present study .Only one of the examined sera was positive for anti‐Yo antibody (1.25% , 1/80 ) and none was positive for anti‐Hu antibodies .This patient suffered from optic neuritis and transverse myelitis , ranging over three vertebral segments on spinal MRI .Conclusions Anti‐Hu and anti‐Yo antibodies were rare in our patients with positive serum AQP4 antibodies .%目的:观察抗 Hu 抗体和抗 Yo 抗体在水通道蛋白‐4(AQP4)抗体阳性患者中的出现情况。方法检测收集80例 AQP4抗体阳性患者,以间接免疫荧光方法检血清抗 Hu 抗体、Yo 抗体,并回顾性分析抗 Hu 抗体、抗 Yo 抗体阳性患者的临床资料及诊断。 AQP4抗体检测:先以转染 AQP4基因的人胚肾293细胞结合血清中的 AQP4抗体,之后采用免疫荧光方法检测;抗 Hu 抗体、抗 Yo 抗体检测:先以猴小脑冰冻切片结合血清中的抗体,之后采用免疫荧光法检测。结果80例患者中,均未检测到抗 Hu 抗体,仅1例 NMO 患者血清抗 Yo 抗体阳性。结论抗 Hu 抗体和抗 Yo 抗体在无肿瘤的 AQP4抗体阳性患者中少见。

  5. Generation of a infectious cDNA clone of highly pathogenic porcine reproductive and respiratory syndrome virus HuN4%高致病性猪繁殖与呼吸综合征病毒HuN4株感染性分子克隆的建立及拯救病毒的鉴定

    Institute of Scientific and Technical Information of China (English)

    张善瑞; 周艳君; 朱建平; 童武; 姜一峰; 童光志

    2011-01-01

    In this study, a infectious cDNA clone of highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) strain HuN4 was generated by cloning of the 6 overlapped RT-PCR fragments into pBlueScript II SK(+), designated pHuN4. In addition, sp6 promoter at 5'-terminus and a Not I site in Poly(A) tail at 3'-terminus were introduced into the cDNA of PRRSV for viral RNA transcription in vitro and the pHuN4 linearization, respectively. Furthmore, the A at 14,680 nt was replaced by G (silent mutation) to generate restriction enzyme Mlu I site as molecular marker for identification of rescued virus. The pHuN4 was linearized and transcribed in vitro, the transcripts were transfected primarily into BHK cells and then Marc 145 cells, the virus was successfully rescued from Marc 145 cells identified by indirect immofluorescent assay and Mlu I digestion. The rescued virus showed similar growth curve to its parental virus HuN4 strain. All the piglets infected with the fifth passage of the rescued virus developed clinical signs at 3 day-past-infection and died within 20 days. The results showed that the rescued virus remained similar biological characterizations as its parental virus. The pHuN4 developed in the study by reverse genetic system might provide a platform for further study on pathogenesis mechanism of highly pathogenic PRRSV.%为构建高致病性猪繁殖与呼吸综合征病毒(PRRSV)感染性克隆,本实验根据PRRSV HuN4株基因组序列设计并合成PRRSV特异性引物,应用RT-PCR技术分6段扩增了PRRSV HuN4株全基因组cDNA.将扩增的各个cDNA部分重叠片段分别克隆于pBlueScript Ⅱ SK(+)载体中构建了感染性重组质粒pHuN4.并在病毒cDNA 5′末端引入sp6启动子序列便于后期的体外转录获得病毒的转录本,在3'末段Poly (A)尾引入Not Ⅰ酶切位点用于线性化pHuN4;此外,将HuN4基因组第14680位的A沉默突变为G产生一个Mlu Ⅰ酶切位点作为鉴定拯救病毒的分子标记.pHu

  6. Further Research on the Date of the First Edition of Hu Sheng Paintings%«护生画集»初版时间再考

    Institute of Scientific and Technical Information of China (English)

    陈星

    2015-01-01

    丰子恺绘作的“护生画”影响深远,但学界对«护生画集»的初版时间却一直表述不清,甚至存在争议。根据弘一法师书信和相关史料,可以厘清«护生画集»的编绘、出版始末,并知晓从«戒杀画集»到«护生画集»这一名称变化的过程。经过考证,可知«护生画集»不可能初版于1928年2月,应初版于1929年1月至7月之间。%Feng Zikai paintings are of profound impact,but there is no clear record about the exact time of the first edition of Husheng Pantings .According to Master Hong Yi’s correspondence and related historical ma-terials,the writer clarified the compilation,publication process of Husheng Pantings ,revealing the whole story of changing the title from "Jiesha Paintins”to Husheng Pantings .After research,the paper proved that Hush-eng Pantings should have been first published in from January to July,1 929,instead of February 1 928.

  7. Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by a new fungal strain Cladosporium cladosporioides Hu-01.

    Science.gov (United States)

    Chen, Shaohua; Liu, Chenglan; Peng, Chuyan; Liu, Hongmei; Hu, Meiying; Zhong, Guohua

    2012-01-01

    Intensive use of chlorpyrifos has resulted in its ubiquitous presence as a contaminant in surface streams and soils. It is thus critically essential to develop bioremediation methods to degrade and eliminate this pollutant from environments. We present here that a new fungal strain Hu-01 with high chlorpyrifos-degradation activity was isolated and identified as Cladosporium cladosporioides based on the morphology and 5.8S rDNA gene analysis. Strain Hu-01 utilized 50 mg·L(-1) of chlorpyrifos as the sole carbon of source, and tolerated high concentration of chlorpyrifos up to 500 mg·L(-1). The optimum degradation conditions were determined to be 26.8°C and pH 6.5 based on the response surface methodology (RSM). Under these conditions, strain Hu-01 completely metabolized the supplemented chlorpyrifos (50 mg·L(-1)) within 5 d. During the biodegradation process, transient accumulation of 3,5,6-trichloro-2-pyridinol (TCP) was observed. However, this intermediate product did not accumulate in the medium and disappeared quickly. No persistent accumulative metabolite was detected by gas chromatopraphy-mass spectrometry (GC-MS) analysis at the end of experiment. Furthermore, degradation kinetics of chlorpyrifos and TCP followed the first-order model. Compared to the non-inoculated controls, the half-lives (t(1/2)) of chlorpyrifos and TCP significantly reduced by 688.0 and 986.9 h with the inoculum, respectively. The isolate harbors the metabolic pathway for the complete detoxification of chlorpyrifos and its hydrolysis product TCP, thus suggesting the fungus may be a promising candidate for bioremediation of chlorpyrifos-contaminated water, soil or crop.

  8. El farmacéutico en el contexto de las enfermedades raras y los medicamentos huérfanos The pharmacist, rare diseases and orphan medicines

    Directory of Open Access Journals (Sweden)

    C. Nagore

    2008-01-01

    Full Text Available El tratamiento de las enfermedades raras muchas veces resulta complicado debido a las dificultades que plantean la investigación y desarrollo de medicamentos huérfanos por parte de la industria farmacéutica. Una de las mayores preocupaciones de este grupo de pacientes tan escaso y diverso al mismo tiempo, es el acceso a los fármacos disponibles. La Agencia Europea del Medicamento establece la denominación de medicamentos huérfanos y los incentivos y bases necesarias para su comercialización. La mayoría de los medicamentos huérfanos por sus especiales características, exigen una adecuada vigilancia y control. Los servicios de farmacia hospitalarios están activamente implicados en su gestión y dispensación, así como en un correcto seguimiento farmacoterapéutico de los pacientes.Treatment of rare diseases is often complicated due to the difficulties posed by the research and development of medicines by the pharmacological industry. One of the greatest concerns of this group of patients, which is both scarce and diverse, is access to available medicines. The European Medicine Agency establishes the term orphan medicines and the incentives and bases for their commercialisation. Due to their special characteristics, the majority of orphan medicines require suitable vigilance and control. The hospital pharmacy services are actively involved in their management and supply, as well as in a correct pharmacotherapeutic monitoring of the patients.

  9. Exploiting a novel miR-519c-HuR-ABCG2 regulatory pathway to overcome chemoresistance in colorectal cancer.

    Science.gov (United States)

    To, Kenneth K W; Leung, W W; Ng, Simon S M

    2015-11-01

    Colorectal cancer (CRC) is a major cause of mortality and morbidity worldwide. While surgery remains the mainstay of treatment for early stage CRC, adjuvant chemotherapy is usually given to reduce the risk of recurrence after colectomy. Overexpression of a multidrug resistance (MDR) transporter ABCG2 in vitro has been shown to cause resistance to 5-fluorouracil (5-FU) and irinotecan, components of the most commonly adopted regimens for treating CRC. Both anticancer drugs are known ABCG2 substrates. An effective way to predict drug response may provide guidance for better cancer treatment. We investigated the effect of ABCG2 dysregulation on cancer cell sensitivity to chemotherapy using pairs of snap-frozen paraffin-embedded archival blocks of human colorectal cancer tissues and their matched non-cancerous colon tissues from CRC patients. In CRC patients responding to chemotherapy, the tumors were found to have remarkable lower ABCG2 expression than the adjacent normal colon tissues. On the contrary, the tumors from patients not responding to 5-FU-based chemotherapy have higher ABCG2 level than the adjacent normal tissues. The high ABCG2 expression in the tumor is associated with the concomitant overexpression of the mRNA binding protein HuR but a low expression of miR-519c because miR-519c is known to target both ABCG2 and HuR. Further investigation in CRC cell lines revealed that the ABCG2 overexpression was caused by an interplay between miR-519c, HuR and the length of the 3' untranslated region (UTR) of ABCG2. These parameters may be further developed as useful biomarkers to predict patient response to adjuvant chemotherapy. Besides being predictive biomarkers, the microRNAs and mRNA binding protein identified may also be potential drug targets for modulating ABCG2 to combat resistance in CRC chemotherapy.

  10. ARG098, a novel anti-human Fas antibody, suppresses synovial hyperplasia and prevents cartilage destruction in a severe combined immunodeficient-HuRAg mouse model

    Directory of Open Access Journals (Sweden)

    Matsubara Tsukasa

    2010-09-01

    Full Text Available Abstract Background The anti-human Fas/APO-1/CD95 (Fas mouse/human chimeric monoclonal IgM antibody ARG098 (ARG098 targets the human Fas molecule. The cytotoxic effects of ARG098 on cells isolated from RA patients, on normal cells in vitro, and on RA synovial tissue and cartilage in vivo using implanted rheumatoid tissues in an SCID mouse model (SCID-HuRAg were investigated to examine the potential of ARG098 as a therapy for RA. Methods ARG098 binding to each cell was analyzed by cytometry. The effects of ARG098 on several cells were assessed by a cell viability assay in vitro. Effects on the RA synovium, lymphocytes, and cartilage were assessed in vivo using the SCID-HuRAg mouse model. Results ARG098 bound to cell surface Fas molecules, and induced apoptosis in Fas-expressing RA synoviocytes and infiltrating lymphocytes in the RA synovium in a dose-dependent manner. However, ARG098 did not affect the cell viability of peripheral blood mononuclear cells of RA patients or normal chondrocytes. ARG098 also induced apoptosis in RA synoviocytes and infiltrating lymphocytes in the RA synovium in vivo. The destruction of cartilage due to synovial invasion was inhibited by ARG098 injection in the modified SCID-HuRAg mouse model. Conclusions ARG098 treatment suppressed RA synovial hyperplasia through the induction of apoptosis and prevented cartilage destruction in vivo. These results suggest that ARG098 might become a new therapy for RA.

  11. Neuroblastoma amplified sequence gene is associated with a novel short stature syndrome characterised by optic nerve atrophy and Pelger-Huët anomaly.

    Science.gov (United States)

    Maksimova, Nadezda; Hara, Kenju; Nikolaeva, Irina; Chun-Feng, Tan; Usui, Tomoaki; Takagi, Mineo; Nishihira, Yasushi; Miyashita, Akinori; Fujiwara, Hiroshi; Oyama, Tokuhide; Nogovicina, Anna; Sukhomyasova, Aitalina; Potapova, Svetlana; Kuwano, Ryozo; Takahashi, Hitoshi; Nishizawa, Masatoyo; Onodera, Osamu

    2010-08-01

    Hereditary short stature syndromes are clinically and genetically heterogeneous disorders and the cause have not been fully identified. Yakuts are a population isolated in Asia; they live in the far east of the Russian Federation and have a high prevalence of hereditary short stature syndrome including 3-M syndrome. A novel short stature syndrome in Yakuts is reported here, which is characterised by autosomal recessive inheritance, severe postnatal growth retardation, facial dysmorphism with senile face, small hands and feet, normal intelligence, Pelger-Huët anomaly of leucocytes, and optic atrophy with loss of visual acuity and colour vision. This new syndrome is designated as short stature with optic atrophy and Pelger-Huët anomaly (SOPH) syndrome. To identify a causative gene for SOPH syndrome. Genomewide homozygosity mapping was conducted in 33 patients in 30 families. The disease locus was mapped to the 1.1 Mb region on chromosome 2p24.3, including the neuroblastoma amplified sequence (NBAS) gene. Subsequently, 33 of 34 patients were identified with SOPH syndrome and had a 5741G/A nucleotide substitution (resulting in the amino acid substitution R1914H) in the NBAS gene in the homozygous state. None of the 203 normal Yakuts individuals had this substitution in the homozygous state. Immunohistochemical analysis revealed that the NBAS protein is well expressed in retinal ganglion cells, epidermal skin cells, and leucocyte cytoplasm in controls as well as a patient with SOPH syndrome. These findings suggest that function of NBAS may associate with the pathogenesis of short stature syndrome as well as optic atrophy and Pelger-Huët anomaly.

  12. Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by a new fungal strain Cladosporium cladosporioides Hu-01.

    Directory of Open Access Journals (Sweden)

    Shaohua Chen

    Full Text Available Intensive use of chlorpyrifos has resulted in its ubiquitous presence as a contaminant in surface streams and soils. It is thus critically essential to develop bioremediation methods to degrade and eliminate this pollutant from environments. We present here that a new fungal strain Hu-01 with high chlorpyrifos-degradation activity was isolated and identified as Cladosporium cladosporioides based on the morphology and 5.8S rDNA gene analysis. Strain Hu-01 utilized 50 mg·L(-1 of chlorpyrifos as the sole carbon of source, and tolerated high concentration of chlorpyrifos up to 500 mg·L(-1. The optimum degradation conditions were determined to be 26.8°C and pH 6.5 based on the response surface methodology (RSM. Under these conditions, strain Hu-01 completely metabolized the supplemented chlorpyrifos (50 mg·L(-1 within 5 d. During the biodegradation process, transient accumulation of 3,5,6-trichloro-2-pyridinol (TCP was observed. However, this intermediate product did not accumulate in the medium and disappeared quickly. No persistent accumulative metabolite was detected by gas chromatopraphy-mass spectrometry (GC-MS analysis at the end of experiment. Furthermore, degradation kinetics of chlorpyrifos and TCP followed the first-order model. Compared to the non-inoculated controls, the half-lives (t(1/2 of chlorpyrifos and TCP significantly reduced by 688.0 and 986.9 h with the inoculum, respectively. The isolate harbors the metabolic pathway for the complete detoxification of chlorpyrifos and its hydrolysis product TCP, thus suggesting the fungus may be a promising candidate for bioremediation of chlorpyrifos-contaminated water, soil or crop.

  13. A novel cantharidin analog N-Benzylcantharidinamide reduces the expression of MMP-9 and invasive potentials of Hep3B via inhibiting cytosolic translocation of HuR

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ji-Yeon; Chung, Tae-Wook; Choi, Hee-Jung [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Lee, Chang Hyun [Department of Anatomy, College of Korean Medicine, Woosuk University, Wanju-gun, Jeonbuk (Korea, Republic of); Eun, Jae Soon; Han, Young Taek [College of Pharmacy, Woosuk University, Wanju-gun, Jeonbuk (Korea, Republic of); Choi, Jun-Yong [Department of Internal Medicine, Korean Medicine Hospital, School of Korean Medicine, Pusan National University, Yangsan 626-870 (Korea, Republic of); Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Kim, So-Yeon; Han, Chang-Woo [Department of Internal Medicine, Korean Medicine Hospital, School of Korean Medicine, Pusan National University, Yangsan 626-870 (Korea, Republic of); Jeong, Han-Sol, E-mail: jhsol33@pusan.ac.kr [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Ha, Ki-Tae, E-mail: hagis@pusan.ac.kr [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of)

    2014-05-02

    Highlights: • We examined the inhibition of N-Benzylcantharidinamide on MMP-9-mediated invasion. • Unlike cantharidin, N-Benzylcantharidinamide has very low toxicity on Hep3B cells. • The reduced MMP-9 expression was due to HuR-mediated decrease of mRNA stability. • We suggest N-Benzylcantharidinamide as a novel inhibitor of MMP-9-related invasion. - Abstract: Invasion and metastasis are major causes of malignant tumor-associated mortality. The present study aimed to investigate the molecular events underlying inhibitory effect of N-Benzylcantharidinamide, a novel synthetic analog of cantharidin, on matrix metalloproteinase-9 (MMP-9)-mediated invasion in highly metastatic hepatocellular carcinoma Hep3B cells. In this investigation, among six analogs of cantharidin, only N-Benzylcantharidinamide has the inhibitory action on MMP-9 expression at non-toxic dose. The MMP-9 expression and invasion of Hep3B cells were significantly suppressed by treatment of N-Benzylcantharidinamide in a dose-dependent manner. On the other hand, the transcriptional activity of MMP-9 promoter and nuclear levels of NF-κB and AP-1 as the main transcriptional factors inducing MMP-9 expression were not affected by it although the level of MMP-9 mRNA was reduced by treatment of N-Benzylcantharidinamide. Interestingly, the stability of MMP-9 mRNA was significantly reduced by N-Benzylcantharidinamide-treatment. In addition, the cytosolic translocation of human antigen R (HuR), which results in the increase of MMP-9 mRNA stability through interaction of HuR with 3′-untranslated region of MMP-9 mRNA, was suppressed by treatment of N-Benzylcantharidinamide, in a dose-dependent manner. Taken together, it was demonstrated, for the first time, that N-Benzylcantharidinamide suppresses MMP-9 expression by reducing HuR-mediated MMP-9 mRNA stability for the inhibition of invasive potential in highly metastatic Hep3B cells.

  14. Reflexiones bioéticas en torno a las enfermedades raras y huérfanas con impacto en el sistema bucodentario

    Directory of Open Access Journals (Sweden)

    Gilberto Cely Galindo

    2011-01-01

    Full Text Available Este artículo está dirigido a odontopediatras que forman parte de grupos interdisciplinarios de investigación y terapia clínica, a los pacientes y sus familias con problemas genéticos de las llamadas "enfermedades raras y huérfanas". Estas enfermedades sistémicas tienen repercusiones severas bucodentales. El énfasis lo ponemos en las reflexiones bioéticas que orientan la acción investigativa y terapéutica.

  15. Did Hu Shi Really Believe in Traditional Chinese Medicine%胡适对中医究竟持什么态度

    Institute of Scientific and Technical Information of China (English)

    祖述宪

    2001-01-01

    胡适是20世纪初中国新文化运动的领袖和思想家,但是,80年代后期以来,我国出版的有关他的生平的著作中,根据胡适所作的“题陆仲安秋室研经图”,认为中医陆仲安早年治愈过他的“糖尿病、慢性肾炎和心脏病”,因而断言胡适相信中医药。仔细检阅胡适的日记、书信和文章,可以得出完全不同的结论。尽管1920—1921年胡适生的那场病,开始时有医生怀疑可能是心脏病或肾炎,但很快就否定了。胡适虽然死于心肌梗塞,但他第一次冠心病发作是1938年底。实际上,中医陆仲安为他治疗的只不过是胡适所说的“小病”,是自限性的,而且胡适一再申明他根本不曾生过糖尿病和慢性肾炎。上述陆仲安治愈胡适糖尿病等疾病的说法纯属以讹传讹,或有意夸张造谣,这件事在中医界至今仍有影响。胡适自称是“信仰西医的人”,并且支持他的友人批评中医,这些批评在今天仍然具有重要的现实意义。%Dr.Hu Shi(or Hu Shih,1891—1962),pioneer of China's New CulturalMovement in the early 20th century,is not only a great thinker but also the leader of the Vernacular Movement and the subsequent literary revolution in China.His progressive and scientific attitude to academic research is epitomized by his advocacy of “dare to propose new hypotheses and then subject them to rigorous and methodical examination”.Several books published since the late 1980's claim that Hu Shi was a believer in traditional Chinese medicine(TCM).The basis for this assertion is his inscription of a painting titled “Lu Zhong-an Studying Medical Classics in Autumn” in 1921,which was done in gratitude after Hu Shi was cured by the practitioner of Chinese medicine Lu Zhong-an of diabetes,chronic nephritis and heart disease.However,a thorough review of Hu Shi's diary,letters and articles reveals that though he suffered his first heart attack in

  16. Nuevas citas y huéspedes de Phenacoccus para la Argentina (Hemiptera: Pseudococcidae New records and host plants of Phenacoccus from Argentina (Hemiptera: Pseudococcidae

    Directory of Open Access Journals (Sweden)

    María C. Granara de Willink

    2003-12-01

    Full Text Available Se cita por primera vez para la Argentina a: Phenacoccus madeirensis Green, Phenacoccus manihoti Matile-Ferrero y Phenacoccus solenopsis Tinsley y se dan a conocer nuevos huéspedes de Phenacoccus crassus Granara de Willink, Phenacoccus parvus Morrison y Phenacoccus similis Granara de Willink.Phenacoccus madeirensis Green, Phenacoccus manihoti Matile-Ferrero and Phenacoccus solenopsis Tinsley, first records from Argentina and new hosts plants of Phenacoccus crassus Granara de Willink, Phenacoccus parvus Morrison y Phenacoccus similis Granara de Willink.

  17. Efecto de una intervención educativa sobre los conocimientos de teniasis/cisticercosis en una comunidad rural de Huánuco, Perú 2005

    OpenAIRE

    Elsa Palacios-Flores; Eler Borneo-Cantalicio

    2008-01-01

    Objetivo. Evaluar el efecto de una intervención educativa sobre los conocimientos de teniasis/cisticercosis en los pobladores de la localidad de Queropalca, Huánuco. Materiales y métodos. Se realizó un ensayo comunitario, en el cual se aplicó una estrategia educativo comunicacional sobre los factores de riesgo de teniasis/cisticercosis, consistente en metodologías participativas de visitas casa en casa y campañas de promoción en toda la jurisdicción durante seis meses (junio a diciembre del 2...

  18. Un mecenas de la educación: Francisco de Solís, fundador del Colegio Menor de Huérfanos

    Directory of Open Access Journals (Sweden)

    Miguel Á. MARTÍN SÁNCHEZ

    2009-11-01

    Full Text Available RESUMEN: Durante el siglo XVI se fundaron en la Universidad de Salamanca multitud de colegios universitarios. Uno de esos colegios, fue el menor de la Concepción de Huérfanos, primer colegio universitario que se funda para amparar niños pobres y huérfanos. El fundador de este colegio fue Francisco de Solís Quiñones y Montenegro, obispo de Bagnorea, secretario y médico particular de Paulo III, entre otras cosas. Este mecenas de la educación, decidió invertir su fortuna en la construcción y dotación de un colegio donde niños pobres y huérfanos, preferiblemente de la ciudad de Salamanca, pudiesen estudiar en la Universidad hasta graduarse en la ciencia que eligiesen. La figura de tan ilustre salmantino ha pasado prácticamente desapercibida, a pesar de su relevancia. Con este trabajo se pretende dar a conocer una de las personalidades más relevantes del panorama renacentista salmantino, por la importancia y trascendencia social y educativa que tuvo su obra.ABSTRACT: During the 16* century, a lot of colleges were founded at the University of Salamanca. One of those colleges, was the Concepción de Huérfanos Minor College, the first college that is founded for protecting poor children and orphans. This college founder was Francisco de Solís Quiñones y Montenegro, bishop of Bagnorea, secretary and personal physician of Paulo III, among other things. This patron of education, decided to spend his fortune on building and endowment of a school where poor children and orphans, in the town of Salamanca preferably, were able to go to university until they graduated in the science they chose. This Salamancan s outstanding figure has gone unnoticed in spite of his importance. In this work, it's tried to introduce one of the most important personalities in Salamancan Renaissance panorama, because of the importance and social and educative implications his work had.

  19. Liver iron is a major regulator of hepcidin gene expression via BMP/SMAD pathway in a rat model of chronic renal failure under treatment with high rHuEPO doses.

    Science.gov (United States)

    Ribeiro, Sandra; Garrido, Patrícia; Fernandes, João; Rocha-Pereira, Petronila; Costa, Elísio; Belo, Luís; Reis, Flávio; Santos-Silva, Alice

    2016-05-01

    Hepcidin is the major central regulator of iron metabolism, controlling iron absorption and mobilization. Considering its interaction with several factors that are altered in chronic kidney disease (CKD), particularly in hyporesponsive CKD patients under therapy with high recombinant human erythropoietin (rHuEPO) doses, it was aimed to study the impact of increasing rHuEPO doses on the regulation of iron-hepcidin metabolism. The blood, cellular, and tissue studies, using the remnant kidney rat model of CKD induced by 5/6 nephrectomy, under rHuEPO (100, 200, 400, and 600 IU/Kg body weight [BW]/week) treatment during 3 weeks were performed. It was found that the rHuEPO stimulus triggered a first wave to achieve correction of anemia, by inhibiting hepcidin synthesis, favoring erythropoiesis and iron absorption; this continuous stimulus enhanced iron absorption leading to iron overload, as showed by the hepatic iron deposits found in rats treated with higher rHuEPO dose that seems to trigger the upregulation of hepcidin synthesis through the activation of the BMP6/SMAD pathway. The data suggested that liver iron overload was an important stimuli for hepcidin synthesis, stronger than the inhibitory effect of high rHuEPO doses; moreover, the findings raised the hypothesis that when high inflammation (triggering hepcidin expression) was associated with increased iron stores in hemodialysis patients, hepcidin expression was also upregulated via BMP6, enhancing hepcidin synthesis, leading, therefore, to worsening of anemia and, eventually, to a hyporesponse/resistance to rHuEPO therapy. © 2016 BioFactors, 42(3):296-306, 2016.

  20. A Brief Analization of the Contextual Beauty of the Black Humor in FENG Xiao-gang's New-year-congradualting Films%冯小刚贺岁电影中黑色幽默的语境美

    Institute of Scientific and Technical Information of China (English)

    陈静怡

    2011-01-01

    The language of the characters in FENG Xiao-gang's New-year-congradulating Films are rich in the expressive force.The outstanding features of his film language lie in the jocosity and jokiness of his characters'dialogues with the thinking of the life philosophy,and thus his unique language style-black humor forms.The exertion of the humor in FENG's films fully depends on the well-composed context by the author.In Feng's films,the importance of context is reflecteted in the different relationgships of the context with other important factors including wording,type of writing,sound,trope,the meaning within or beyond the language itself and so on.No other factors than the organic combination of black humor with its context can help us taste the beauty of context in the language with black humor,enjoy the black humor in the beauty of context,and accordingly further understand the author's intention and leitmotiv.%冯小刚贺岁作品中的人物语言极富表现力。其电影语言的特性表现在人物对白的诙谐性和调侃性上,幽默中带有生活哲理的思考,形成了黑色幽默的语言风格。综观其作品,语境的重要性主要体现于语境和用词、语体、语音、修辞、意内言外诸要素的关系中。正是由于黑色幽默和语境的有机结合,才让我们在黑色幽默的语言中感受语境美,在语言的语境美中品味黑色幽默,从而更好地理解作者的创作意图和主旨。

  1. Loss of Genetic Diversity of Domesticated Panax notoginseng F H Chen as Evidenced by ITS Sequence and AFLP Polymorphism: A Comparative Study with P. stipuleanatus H T Tsai et K M Feng

    Institute of Scientific and Technical Information of China (English)

    Shi-Liang ZHOU; Gao-Ming XIONG; Zhong-Yi LI; Jun WEN

    2005-01-01

    In the present study, we evaluated the genetic diversity of Panax notoginseng F H Chen, a domesticated species, and P. stipuleanatus H T Tsai et K M Feng, an endangered wild species in southeastern Yunnan and adjacent areas in Vietnam, using sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA and amplified fragment length polymorphism (AFLP) markers. Twenty-four accessions from three plantations of P. notoginseng and 51 samples from eight populations of P. stipuleanatus were assayed. A total of 694 bp of partial sequences of 18S, ITS 1, 5.8S, ITS2, and partial sequences of 26S were obtained. No sequence variation was detected within P. notoginseng and nine sites (1.30%) were variable in P. stipuleanatus. Two-thirds of the variable sites were found between Langqiao and other populations. In P. notoginseng, four pairs of AFLP primer combinations generated 312 bands, of which 240 (76.9%) were polymorphic and 60.15% of the polymorphisms were harbored within plantations. Approximately 41.0% and 66.9% of bands were polymorphic in population D7 and 5589, respectively. In P.stipuleanatus, the same four primer combinations produced 346 bands, of which 334 (96.5%) were polymorphic and approximately 62.14% of polymorphisms were maintained within populations. Considerable variations were observed. The percentage of polymorphic bands ranged from 50.2% to 84.9% and the average over populations was 70.9%. Cluster analysis did not show correlation of genetic differentiation with the distinctive leaf morphology of P. stipuleanatus (i.e. one form with bipinnatifid leaflets and the other with undivided leaflets). Because over 40% of genetic variations were maintained among populations and because of the very restricted distribution of P. stipuleanatus, all natural populations of this species should be conserved in situ. Considering that there are variations in P. notoginseng within and among plantations, we suggest establishing a genetic resource

  2. Determination of the Amino Acids Content in the BaFeng Amino Acids Oral Liquid with Accq.Tag Method%Accq. Tag法测定氨基酸口服液的氨基酸含量

    Institute of Scientific and Technical Information of China (English)

    陈永波; 汪大顺; 覃建华; 饶斌; 覃兰

    2001-01-01

    采用AccQ.Tag法对氨基酸口服液中游离氨基酸和牛磺酸含量 进行了分离测定。产品中氨基酸总量达85 mg/ml以上,共13种氨基酸。必需氨基酸与非必需 氨基酸比例为2.20~2.50∶1,支/芳比为2.30~2.55∶1.配方接近于FAO氨基酸比例模式,以 FAO氨基酸模式及化学评分评价了该制剂的营养价值,基本上不存在限制氨基酸,化学评分 均在90分以上。%The separation of the Taurine and Determintion of the f ree amino acids content in BaFeng Amino Acids Oral Liquid with AccQ.Tag mothod was described. The total amino acids contents are more than 8500 mg/100 ml. Ther e were thirteen sorts of free amino acids in this product indicating that the pr oduct was made of crystal free amino acids. The ratio of essential amino acids a nd other amino acids was between 2.20:1and 2.50∶1, the ratio of brench-chain amino acids and sweet-scented amino acids was 2.30--2.55∶1. The patterns of am ino acids component is near to the pattern recommended by FAO. According to the ratios and the patterns of amino acids recommended by FAO, the nutritive values of the product were evaluated. From the results, it was found that there was no limiting amino acids and the chemical scor of amino acids was more than ninety-f ive.

  3. Ways of the World and the Life——On the Flavor of Folk Custom Culture of Feng Zikai's prose creation%世态人生皆风情——论丰子恺散文创作的民俗文化元素

    Institute of Scientific and Technical Information of China (English)

    刘丽琼

    2011-01-01

    Feng Zikai's prose creation involves the social life,containing the rich flavor of folk custom culture,especially the prose recalling hometown people and affairs and the diaries describing what he saw and heard in Guilin in the Sino-Japanese War.Feng Zika%丰子恺的散文创作涉及社会人生的方方面面,蕴含着丰富的民俗文化元素,特别是在对故乡人事的回忆和描摹的散文以及在抗战时期逃难桂林的见闻日记里,丰子恺以清新趣味的笔调为我们描绘了一幅幅民情风俗画卷。丰子恺散文创作的民俗文化元素具体体现在,通过对饮食、方言俗语、人物称谓、岁时节日、信仰与禁忌等民俗文化元素的运用,构成他的散文的一些主要情节,揭示人物性格,营造环境氛围,体现作品浓厚的地方特色和传统文化底蕴。

  4. Tafonomia del yacimiento de vertebrados pleistoceno de Huéscar-1 (Granada, españa

    Directory of Open Access Journals (Sweden)

    Alberdi, M. T.

    2003-08-01

    Full Text Available This work analyses the sedimentary and taphonomic processes that induced the selective preservation and spatial distribution of Húescar-1 bone assemblage (early Galerian. The bone fossil remains are associated with an alluvial fan system that flowed into a lacustrine area with calcareous sedimentation. The fossils show numerous scratch and abrasion marks produced during their transportation as channel lag. The remains appear in three sedimentological settings: in paleochannel filling-up sequences, in pockets built at the outlet of the channels in the lacustrine area, and scattered within calcareous silts. Nevertheless, most of them are found in the second instante. The cumulative process was attritional as pointed out by the mortality profiles of Equus altidens and could be explained by selective predation and/or seasonal bone accumulation. The notably small number of artiodactyl species, particularly in the typical foms of bush or forest habitats and the presence of amphibious forms (Hippopotamus are indicative of an open grassland habitat. The aquatic birds represent the autochthonous component of the association. For each bone recovered severa1 variables were measured, the data obtained underwent graphic and statistical treatment that provided useful information for the site taphonomic interpretation.Se estudia el conjunto de procesos sedimentarios y rasgos tafonómicos que condicionaron la preservación selectiva y disposición espacial de los restos de vertebrados que forman el yacimiento de edad Galeriense inferior de Huéscar-l. Los restos óseos están asociados a un sistema de abanicos aluviales que desembocaba en una zona lacustre carbonatada amplia. Es un conjunto transportado donde los restos muestran numerosas señales de abrasión y arrastre. Los restos óseos aparecen en tres situaciones sedimentológicas: carga de fondo en el relleno de los paleocanales, bolsadas formadas en el punto de la desembocadura y dispersos espor

  5. A frameshift mutation in the HuP2 paired domain of the probable human homolog of murine Pax-3 is responsible for Waardenburg syndrome type 1 in an Indonesian family.

    Science.gov (United States)

    Morell, R; Friedman, T B; Moeljopawiro, S; Hartono; Soewito; Asher, J H

    1992-07-01

    Waardenburg syndrome type 1 (WS1) is an autosomal dominant disorder characterized by deafness, dystopia canthorum, heterochromia iridis, white forelock, and premature greying. A similar phenotype is caused in the mouse by mutations in the Pax-3 gene. This observation, together with comparisons of conserved syntenies in the murine and human genetic maps, suggested that at least some WS1 mutations should occur in HuP2, the probable human homolog of Pax-3. Two mutations in the HuP2 sequence of individuals with WS1 have been reported recently. Both of them occur in the highly conserved paired box region of the gene, which encodes a DNA binding domain. The functional consequences of these mutations are at present speculative. We report here a 14 bp deletion in the paired domain encoded by exon 2 of HuP2 in an Indonesian family segregating for WS1. This frameshift mutation results in a premature termination codon in exon 3. The HuP2 product is a truncated protein lacking most of the paired domain and all of the predicted homeo domain. We propose that the WS1 phenotype in this family is due to loss of function of HuP2 and discuss two mechanisms for the dominant effect of this mutation.

  6. Tissue-specific promoter usage and diverse splicing variants of found in neurons, an ancestral Hu/ELAV-like RNA-binding protein gene of insects, in the direct-developing insect Gryllus bimaculatus.

    Science.gov (United States)

    Watanabe, T; Aonuma, H

    2014-02-01

    Hu/ELAV-like RNA-binding proteins (RBPs) are involved in the post-transcriptional regulation of RNA metabolism including splicing, transport, translational control and turnover. The Hu/ELAV-like RBP genes are predominantly expressed in neurons, and are therefore used as common neuronal markers in many animals. Although the expression patterns and functions of the Hu/ELAV-like RBP genes have been extensively studied in the model insect Drosophila melanogaster, little is known in basal direct-developing insects. In the present study, we performed an identification and expression analysis of the found in neurons (fne) gene, an ancestral insect Hu/ELAV-like RBP gene, in the cricket Gryllus bimaculatus. Contrary to expectation that the Gryllus fne transcript would be predominantly expressed in the nervous system, expression analysis revealed that the Gryllus fne gene is expressed broadly. In addition, we discovered that alternative promoter usage directs tissue-specific and embryonic stage-dependent regulation of fne expression, and that alternative splicing contributes to the generation of diverse sets of fne transcripts. Our data provide novel insights into the evolutionary diversification of the Hu/ELAV-like RBP gene family in insects. © 2013 The Royal Entomological Society.

  7. HU Sirong distinguishes key points of treating cor pulmonale heart failure%胡思荣辨治肺心病心衰

    Institute of Scientific and Technical Information of China (English)

    左明晏

    2016-01-01

    胡思荣教授从事中、西医临床及科研工作40余年,积累了丰富的医疗经验。胡师精于仲景学说,兼通温病各家诸说,擅长运用相关理论辨治肺心病心衰。提倡从痰饮论治肺心病心衰,认为肺心病阳虚为本,痰饮为基本病因,以温化痰饮为主--务,灵活运用温药除其湿,采用中病既止之法则。%Professor HU Sirong engaged in clinical and scientific research between Chinese and western medicine more than 40 years,has accumulated rich experience in health care. Hu teacher skilled in zhang zhongjing theory,and warm disease all said,are good at using relevant theory of treating cor pulmonale heart failure. Advocates from phlegm retention differentiation of cor pulmonale heart failure,thought that Yang deficiency and phlegm retention as the basic etiology,phlegm drink warm as the main priority,and flexible use of medicine in addition to the wet warm,adopt the rules of disease in both check.

  8. El zorro Andino, P seudalopes P seudalopes P culpaeus , un nuevo huésped para Corynosoma obtuscens (Acanthocephala en el Perú*

    Directory of Open Access Journals (Sweden)

    Manuel Tantaleán

    2013-04-01

    Full Text Available Se describe la presencia de Corynosoma obtuscens Lincicome, 1943 (Acanthocephala, Polymor- Corynosoma obtuscens L i n c i c o m e , 1 9 4 3 ( A c a n t h o c e p h a l a , P o l y m o r - Corynosoma obtuscens phidae en el intestino delgado del zorro andino Pseudalopex culpaeus (Molina, 1782 procedente Pseudalopex culpaeus ( M o l i n a , 1 7 8 2 p r o c e d e n t e Pseudalopex culpaeus de San Juan de Marcona, Departamento de Ica, Perú. Esta es la segunda vez que se registra la infección natural de un mamífero terrestre por C. obtuscens en el Perú, por lo que se considera C. obtuscens en el Perú, por lo que se considera C. obtuscens a P. culpaeus como huésped defi nitivo accidental P. culpaeus como huésped defi nitivo accidental P. culpaeus ; además se comenta la posibilidad de que éste acantocefalo infecte al hombre.

  9. Spatio-Temporal Patterns of Urban-Rural Development and Transformation in East of the “Hu Huanyong Line”, China

    Directory of Open Access Journals (Sweden)

    Zhichao Hu

    2016-02-01

    Full Text Available Urban-rural development and transformation is profoundly changing the socioeconomic system as well as the natural environment. The study uses the AHP (Analytic Hierarchy Process method to construct a top-down index of human activity based around five dimensions (population, land, industry, society, and environment to evaluate the spatial characteristics in the region east of the Hu Huanyong line, China, in 1994 and 2010. Then, we investigate the spatial-temporal pattern using the methods of hotspot analysis, local Moran’s I index and Pearson correlation coefficient. The calculation showed that: (1 northeast China was experiencing an economic recession during study period, and the implementation of revitalization plan have not controlled the recession trend yet; (2 Pearson correlation analysis showed that the improvement of population quality promote the development of industry and society systems significantly during study period; and (3 negative correlation between Population Development Index (PDI change and Population Transformation Index (PTI change (along with the Society Transformation Index (STI change and Industry Transformation Index (ITI change reflected that east of the Hu Huanyong line, China was in a “demographic dividend” period. Then, with the help of SOFM neural network algorithm, we divided the study area into six types of region, and found that municipalities, provincial capitals, Yangtze River Delta region and cities on the North China Plain owned the greatest development, while cities in southwest and northeast China showed relatively poor development during study period.

  10. Hepatitis B Virus X Protein Stimulates Proliferation, Wound Closure and Inhibits Apoptosis of HuH-7 Cells via CDC42

    Science.gov (United States)

    Xu, Yongru; Qi, Yingzi; Luo, Jing; Yang, Jing; Xie, Qi; Deng, Chen; Su, Na; Wei, Wei; Shi, Deshun; Xu, Feng; Li, Xiangping; Xu, Ping

    2017-01-01

    Chronic hepatitis B virus (HBV) infection has been considered as the major cause of hepatocellular carcinoma (HCC). Hepatitis B virus X protein (HBx) has been reported to be oncogenic. The underlying mechanisms of HBV-related HCC are not fully understood, and the role played by the HBx protein in HBV induced carcinogenesis remains controversial. CDC42, a member of the Rho GTPase family, has been reported to be overexpressed in several different cancers, including HBV-related HCC. However, the specific role of CDC42 in HCC development remains unclear. Here, we investigated the cellular mechanisms by which CDC42 was responsible for the higher proliferation of HuH-7 cells mediated by HBx. We found that the expression level of CDC42 and its activity were significantly increased in HuH-7-HBx cells. The deficiency of CDC42 using the CRISPR/Cas9 system and inhibition by specific inhibitor CASIN led to the reduction of HBx-mediated proliferation. Furthermore, we observed that IQ Motif Containing GTPase Activating Protein 1 (IQGAP1), the downstream mediator of the CDC42 pathway, might be involved in the carcinogenesis induced by HBx. Therefore, the HBx/CDC42/IQGAP1 signaling pathway may potentially play an important role in HBx-mediated carcinogenesis. PMID:28282856

  11. Analysis of the spatiotemporal expression of major genes in the TGF-β/Smad signaling pathway and correlation analysis using Hu sheep muscle tissue.

    Science.gov (United States)

    Wang, Q Z; Su, R; Lv, X Y; Gao, W; Chen, L; Bao, J J; Yu, J R; Wang, L H; Sun, W

    2016-05-23

    The mRNA expression levels of key genes (Smads, MSTN, and MyoG) in the TGF-β/Smad signaling pathway in Hu sheep at different growth stages (2 days, 2 months, and 6 months of age) and in different skeletal muscles (longissimus dorsi muscle and soleus muscle) and different genders were detected; and correlation of the Smad family (Smad2, Smad3, Smad4, and Smad7), MSTN, MyoG expressions was analyzed in Hu sheep. The results showed that the expression of Smads was higher in the soleus muscle than in the longissimus dorsi muscle; the expressions of Smad2, Smad3, and Smad4 were significantly higher in 2-day-old sheep than in sheep belonging to the other age groups (P muscle tissues, expression of Smad2 was significantly positively correlated (P < 0.01) with that of Smad3. The expression of Smad3 was significantly positively correlated (P < 0.01) with that of Smad4, which showed that the Smad family genes could have an inhibitory effect on the TGF-β/Smad signaling pathway.

  12. HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA

    Directory of Open Access Journals (Sweden)

    Wenhui Zhao

    2014-02-01

    Full Text Available SIRT1 is a pleiotropic protein that plays critical and multifunctional roles in metabolism, senescence, longevity, stress-responses, and cancer, and has become an important therapeutic target across a range of diseases. Recent research demonstrated that SIRT1 pre-mRNA undergoes alternative splicing to produce different isoforms, such as SIRT1 full-length and SIRT1-∆Exon8 variants. Previous studies revealed these SIRT1 mRNA splice variants convey different characteristics and functions to the protein, which may in turn explain the multifunctional roles of SIRT1. However, the mechanisms underlying the regulation of SIRT1 alternative splicing remain to be elucidated. Our objective is to search for new pathways that regulate of SIRT1 alternative splicing. Here we describe experiments showing that HuR and TIA1/TIAL1, two kinds of RNA-binding proteins, were involved in the regulation of alternative splicing of SIRT1 pre-mRNA under normal and stress circumstances: HuR increased SIRT1-∆Exon8 by promoting SIRT1 exon 8 exclusion, whereas TIA1/TIAL1 inhibition of the exon 8 exclusion led to a decrease in SIRT1-∆Exon8 mRNA levels. This study provides novel insight into how the alternative splicing of SIRT1 pre-mRNA is regulated, which has fundamental implications for understanding the critical and multifunctional roles of SIRT1.

  13. Feng shui pa-kua / Ann Tenno

    Index Scriptorium Estoniae

    Tenno, Ann, 1952-

    2003-01-01

    Pa-kua sümbol, kolmikmärkide Varane Taevane Järjestus - yin pa-kua ja Hilisem Taevane Järjestus - yang pa-kua. Inimeste kodude - majade ja aedade kujundamiseks kasutatakse Hilisema Taevase Järjestuse pa-kua'd. Pa-kua kolmikmärgid yang pa-kua järjestuses, soovitusi aia kujundamiseks. 8 ill

  14. Armastuse feng shui / Peeter Rüberg

    Index Scriptorium Estoniae

    Rüberg, Peeter

    2000-01-01

    Järg Jan/13;20;27/Feb/3;10;17;23/Mar/2;9;16 ;30/Apr/13/May/4;11 lk. 22;38;56;70;87;102;118;134;150;166;198;230;278 ;296Vanal hiina kodu- ja elukunstil on igaks armuelu juhtumiks varuks praktilisi nõuandeid ja võtteid

  15. Feng shui pa-kua / Ann Tenno

    Index Scriptorium Estoniae

    Tenno, Ann, 1952-

    2003-01-01

    Pa-kua sümbol, kolmikmärkide Varane Taevane Järjestus - yin pa-kua ja Hilisem Taevane Järjestus - yang pa-kua. Inimeste kodude - majade ja aedade kujundamiseks kasutatakse Hilisema Taevase Järjestuse pa-kua'd. Pa-kua kolmikmärgid yang pa-kua järjestuses, soovitusi aia kujundamiseks. 8 ill

  16. Armastuse feng shui / Peeter Rüberg

    Index Scriptorium Estoniae

    Rüberg, Peeter

    2000-01-01

    Järg Jan/13;20;27/Feb/3;10;17;23/Mar/2;9;16 ;30/Apr/13/May/4;11 lk. 22;38;56;70;87;102;118;134;150;166;198;230;278 ;296Vanal hiina kodu- ja elukunstil on igaks armuelu juhtumiks varuks praktilisi nõuandeid ja võtteid

  17. From the Ethnic History of Asia – the Dōnghú, Wūhuán and Xiānbēi Proto-Mongolian Tribes

    Directory of Open Access Journals (Sweden)

    Nenad Vidaković

    2012-04-01

    Full Text Available The aim of this paper is to present the history of the Dōnghú, Wūhuán and Xiānbēi Proto-Mongolian tribes in the period from the 4th century B.C. to the end of the 3rd century A.D. The history of the ancient nomadic peoples who lived north of China is written in Chinese dynasty chronicles. Proto-Mongolian tribes from the 1st century B.C. are called Dōnghú in Chinese sources. The earliest news on them originates from the Warring States Period (4th – 3rd century B.C., and tells of a conflict with the northern Chinese states. Other types of sources on the history of the Proto-Mongolian tribes are archaeological findings, which associate Mongolian ethnogenesis with slab grave cultures and the Lower Xiàjiādiàn. Linguists find the materials for the research on Mongolian ethnogenesis in the Altaic linguistic family, which the Mongolian language belongs to as well. Based on the mentioned sources, the change in the political situation in the steppes at the end of the 3rd century B.C., when the people of Xiōngnú created a powerful state and conquered the Dōnghúes, is described in the paper. The remains of the shattered Dōnghúes, who had mostly migrated to the north, have been recorded in Chinese chronicles under new topoethnonyms: Xiānbēi and Wūhuán. The weakening and fall of the Xiōngnúes’ state enabled the Proto-Mongolian tribes to re-enter the historical scene. At the end of the 1st century B.C. the Chinese Hàn Empire firstly established relations with the Wūhuán tribes and in the middle of the 1st century A.D. with the Xiānbēi tribes, too. In the beginning both tribal alliances acknowledged the supreme authority of China and carried out frontier service. Under the guidance of tribal chiefs the tribes started to run an independent policy and attack China’s border areas during the 2nd century A.D. In the conclusion, the author describes the period when the Wūhuán and Xiānbēi tribes were at the peak of their power

  18. Les risques conchylicoles en Baie de Quiberon. Première partie : le risque de mortalité virale du naissain d’huître creuse Crassostra gigas. Rapport final du projet Risco 2010-2013

    OpenAIRE

    Mazurie, Joseph; Stanisiere, Jean-yves; Langlade, Aime; Bouget, Jean-francois; Dumas, Franck; Treguier, Cathy; Leclerc,Emilie; Ravaud, Emilie; Quinsat, Kevin; Gabellec, Raoul; Retho, Michael; Cochet, Helene; Dreano, Alain

    2013-01-01

    L’étude « Risco », labellisée Pôle Mer et financée par la Région Bretagne, mobilise à la fois des socio-économistes, des biologistes et des professionnels. Elle vise à comprendre et gérer les facteurs de mortalités massives d’huîtres creuses (Crassostrea gigas) enregistrées par les concessionnaires de baie de Quiberon (France, 56), à partir de 2006 sur les huîtres adultes et 2008 sur le naissain. Le protocole engagé en 2010, avec un volet expérimental basé sur le suivi mensuel de lots d’huîtr...

  19. 利用微卫星标记分析湖羊GDF8区域的遗传多样性%Genetic Polymorphism in GDF8 Region of HU Sheep Based on Microsatellite DNA

    Institute of Scientific and Technical Information of China (English)

    吴文忠; 钱建共; 陈玲; 孙伟

    2009-01-01

    [Objective] This study was to analyze the genetic polymorphism in GDF8 Region of HU sheep. [Method] Four microsatellite loci including BMS1591, TEXAN-2, FCB128 and BM81124 mapped on GDF8 region of chromosome No. 2 of sheep that may be correlated with growth performance were chosen to detect the molecular genetics foundation of growth performance of Hu sheep. [Result] Four microsatellite loci detected were high in heterozygosity, more in effective alleles number and rich in polymorphic information, all the three indices passed through the high polymorphic level (PIC>0.5). [Conclusion] The four microsatellite loci detected could be used to estimate the genetic polymorphism of growth performance of Hu sheep.

  20. The RNA-binding protein HuD is required for GAP-43 mRNA stability, GAP-43 gene expression, and PKC-dependent neurite outgrowth in PC12 cells.

    Science.gov (United States)

    Mobarak, C D; Anderson, K D; Morin, M; Beckel-Mitchener, A; Rogers, S L; Furneaux, H; King, P; Perrone-Bizzozero, N I

    2000-09-01

    The RNA-binding protein HuD binds to a regulatory element in the 3' untranslated region (3' UTR) of the GAP-43 mRNA. To investigate the functional significance of this interaction, we generated PC12 cell lines in which HuD levels were controlled by transfection with either antisense (pDuH) or sense (pcHuD) constructs. pDuH-transfected cells contained reduced amounts of GAP-43 protein and mRNA, and these levels remained low even after nerve growth factor (NGF) stimulation, a treatment that is normally associated with protein kinase C (PKC)-dependent stabilization of the GAP-43 mRNA and neuronal differentiation. Analysis of GAP-43 mRNA stability demonstrated that the mRNA had a shorter half-life in these cells. In agreement with their deficient GAP-43 expression, pDuH cells failed to grow neurites in the presence of NGF or phorbol esters. These cells, however, exhibited normal neurite outgrowth when exposed to dibutyryl-cAMP, an agent that induces outgrowth independently from GAP-43. We observed opposite effects in pcHuD-transfected cells. The GAP-43 mRNA was stabilized in these cells, leading to an increase in the levels of the GAP-43 mRNA and protein. pcHuD cells were also found to grow short spontaneous neurites, a process that required the presence of GAP-43. In conclusion, our results suggest that HuD plays a critical role in PKC-mediated neurite outgrowth in PC12 cells and that this protein does so primarily by promoting the stabilization of the GAP-43 mRNA.

  1. 胡锦涛的公民政治参与思想%Hu Jingtao's Ideology of Citizens' Politic Participation

    Institute of Scientific and Technical Information of China (English)

    杨平

    2012-01-01

    Hu Jingtao's ideology of citizens' politic participation is the same with that of Marxist revolutionary teachers and the different leading generations of the CPC.Hu Jingtao thinks that people's democracy is soul of socialism.We should broadly persuade people to join in the management of public affairs.He thinks citizens' participation should be in line with the reform of politic system.We should strengthen participating degree for the citizens in joining in public policy-making,and make full use of democratic parties and social associations in citizens' politic participation.We should enlarge the orderly politic participation by putting forward basic democracy and guarantee ordinary Party members' politic participation by putting forward democracy inside the Party.Hu Jingtao's ideology of citizens' politic participation is the focusing reflection of the new achievement from Marxist theory of political participation.%胡锦涛的公民政治参与思想是和马克思主义革命导师以及中国共产党历代领导人的政治参与理论一脉相承的。胡锦涛认为,人民民主是社会主义的生命,要广泛动员人民参加公共事务的管理,公民政治参与要和政治体制改革相适应,要提高公民对公共决策的参与度,发挥民主党派和社会组织在公民政治参与中的作用,要通过发展基层民主扩大公民有序政治参与,通过发展党内民主保障普通党员的政治参与权。胡锦涛的公民政治参与思想是马克思主义政治参与理论中国化最新成果的集中体现。

  2. DOSoReMI.hu: collection of countrywide DSM products partly according to GSM.net specifications, partly driven by specific user demands

    Science.gov (United States)

    Pásztor, László; Laborczi, Annamária; Takács, Katalin; Szatmári, Gábor; Illés, Gábor; Bakacsi, Zsófia; Szabó, József

    2017-04-01

    Due to former soil surveys and mapping activities significant amount of soil information has accumulated in Hungary. In traditional soil mapping the creation of a new map was troublesome and laborious. As a consequence, robust maps were elaborated and rather the demands were fitted to the available map products. Until recently spatial soil information demands have been serviced with the available datasets either in their actual form or after certain specific and often enforced, thematic and spatial inference. Considerable imperfection may occur in the accuracy and reliability of the map products, since there might be significant discrepancies between the available data and the expected information. The DOSoReMI.hu (Digital, Optimized, Soil Related Maps and Information in Hungary) project was started intentionally for the renewal of the national soil spatial infrastructure in Hungary. During our activities we have significantly extended the potential, how soil information requirements could be satisfied. Soil property, soil type as well as functional soil maps were targeted. The set of the applied digital soil mapping techniques has been gradually broadened incorporating and eventually integrating geostatistical, data mining and GIS tools. Soil property maps have been compiled partly according to GSM.net specifications, partly by slightly or more strictly changing some of their predefined parameters (depth intervals, pixel size, property etc.) according to the specific demands on the final products. The elaborated primary maps were further processed, since even DOSoReMI.hu intended to take steps for the regionalization of higher level soil information (processes, functions, and services) involving crop models in the spatial modelling. The framework of DOSoReMI.hu also provides opportunity for the elaboration of goal specific soil maps, with the prescription of the parameters (thematic, resolution, accuracy, reliability etc.) characterizing the map product. As a

  3. 提升连云港市城市品牌影响力的路径选择--以雷锋车精神新的时代内涵为切入点%Lianyungang City Brand Influence Route Choice:To Lei Feng car Spirit Connotation as the Breakthrough Point of the New Era

    Institute of Scientific and Technical Information of China (English)

    刘丽; 王亚光

    2013-01-01

    All over the country cities in building its own brand,in order to enhance in the national and global influence.Lianyungang city invested a lot of energy,actively promote its superior geographical loca-tion,unique scenery,the rich product resources,the formation of a certain influence,but the propaganda lack of humanistic spirit.Especially for the spirit of lianyungang city unique LeiFengChe refining,packa-ging,promotion is not enough,not the Lei Feng car spirit as an important part of city brand construction system is considered.Is the basic characteristic and important role of city brand and incorporate LeiF-engChe spirit especially the connotation of the new period,the necessity and importance of urban brand construction,and how to LeiFengChe spirit for further refining,packaging,promotion and further carry forward measures were studied.%全国各地城市都在打造各自的城市品牌,以提升在全国乃至全球的影响力。连云港市投入了大量的精力,积极宣传其优越的地理位置、独特的山海风光、丰富的物产资源,形成了一定的影响力,但对人文精神的宣传力度欠缺。特别是对于连云港市独有的雷锋车精神提炼、包装、推广不够,没有把雷锋车精神作为城市品牌建设的重要组成部分加以系统考虑。就城市品牌的基本特征及重要作用,将雷锋车精神特别是其新时期的内涵纳入城市品牌建设的必要性和重要性,以及如何将雷锋车精神进行进一步提炼、包装、宣传和进一步弘扬的措施进行了研究。

  4. New Definition of Micro-Lecture:Walking from Technology Development to Effective Design-An Interview to Pro.Hu Xiaoyong from South China Normal University and Mr.Hu Tiesheng from Foshan Education Bureau%微课新界定:从技术开发迈向有效设计--访华南师范大学胡小勇教授和佛山教育局胡铁生老师

    Institute of Scientific and Technical Information of China (English)

    冯智慧; 郑晓丹

    2015-01-01

    Leaderette:In recent years,micro-lecture has become a big event in the field of education and "nationwide micro-lecture" phenomenon almost emerged.How to understand and define the characteristics of micro-lecture?How to recognize the function of micro-lecture rationally?How to go beyond from micro-lecture technology development to effective design?In order to answer these questions,we interview Professor Hu Xiaoyong and Mr.Hu Tiesheng to introduce the new thinking in the micro-lecture tide to us.

  5. Ultra high performance liquid chromatography with tandem mass spectrometry for rapid separation and identification of main constituents in Chinese herbal formula Xuan Hu Suo San.

    Science.gov (United States)

    Tang, Huaqi; Zhang, Xinyue; Luo, Shilin; Shang, Fei; Chen, Jianping; Wang, Jinghong; Duan, Xiaojie; Gao, Xiaoyan; Sun, Yikun

    2017-02-15

    Xuan Hu Suo San (XHSS) is a traditional Chinese medicine that has been extensively applied in the treatment of osteoarthritis for many years, however, its chemical composition has not yet been elucidated. Thus, a rapid, efficient, and precise method based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was applied in both positive- and negative-ion modes to rapidly separate and identify the main chemical ingredients in XHSS for the first time. Finally, with the optimised separation and detection method, a total of 57 compounds were simultaneously separated within 13 min, among which 14 compounds were confirmed by comparing retention time and MS data with reference standards and others were tentatively identified by comparing with reference literatures. This rapid and sensitive approach is highly useful for the identification and characterisation of chemical constituents, and provides fundamental and extensive information supporting further metabolic and pharmacokinetic studies of XHSS.

  6. Acentuación de la enfermedad experimental de injerto contra huésped con el agregado de esplenocitos murinos

    OpenAIRE

    2010-01-01

    En un trabajo anterior se presentó en un modelo de Enfermedad de Rechazo de Injerto vs Huésped (EICH) en ratones ablacionados con ciclofosfamida y trasplantados con células de médula ósea con hemi-disparidad genética una alteración del ciclo celular, con detención en la fase G2-M de las células del epitelio intestinal y de la piel de ratones, asociada a un aumento de la apoptosis. Con el fin de intentar profundizar en los mecanismos de producción de daño epitelial en la EICH se modificó el mo...

  7. Essais de prégrossissement de l'huître plate (Ostrea edulis) à partir de naissain capté en Méditerranée

    OpenAIRE

    Dupuy, Blandine

    1988-01-01

    La maîtrise du prégrossissement d'huîtres plates s'avère indispensable pour valoriser sur le littoral Languedocien le naissain capté en mer ouverte. Cet essai effectue la comparaison de trois sites : - en mer ouverte à - 20 mètres à Gruissan sur une concession mytilicole. - en étang à proximité d'une zone d'élevage de palourdes à Port-la-Nouvelle. - en bassin aménagé pour l'élevage de crevettes dans les marais de Campignol. A partir de naissain de 0,5 cm en mai-juin, on a obtenu d...

  8. A dual model HU conversion from MRI intensity values within and outside of bone segment for MRI-based radiotherapy treatment planning of prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Korhonen, Juha, E-mail: juha.p.korhonen@hus.fi [Clinical Research Institute Helsinki University Central Hospital Ltd., POB-700, 00029 HUS (Finland); Department of Oncology, Helsinki University Central Hospital, POB-180, 00029 HUS (Finland); Kapanen, Mika [Clinical Research Institute Helsinki University Central Hospital Ltd., POB-700, 00029 HUS (Finland); Department of Oncology, Helsinki University Central Hospital, POB-180, 00029 HUS (Finland); Department of Medical Physics, Tampere University Hospital, POB-2000, 33521 Tampere (Finland); Keyriläinen, Jani; Seppälä, Tiina; Tenhunen, Mikko [Department of Oncology, Helsinki University Central Hospital, POB-180, 00029 HUS (Finland)

    2014-01-15

    Purpose: The lack of electron density information in magnetic resonance images (MRI) poses a major challenge for MRI-based radiotherapy treatment planning (RTP). In this study the authors convert MRI intensity values into Hounsfield units (HUs) in the male pelvis and thus enable accurate MRI-based RTP for prostate cancer patients with varying tissue anatomy and body fat contents. Methods: T{sub 1}/T{sub 2}*-weighted MRI intensity values and standard computed tomography (CT) image HUs in the male pelvis were analyzed using image data of 10 prostate cancer patients. The collected data were utilized to generate a dual model HU conversion technique from MRI intensity values of the single image set separately within and outside of contoured pelvic bones. Within the bone segment local MRI intensity values were converted to HUs by applying a second-order polynomial model. This model was tuned for each patient by two patient-specific adjustments: MR signal normalization to correct shifts in absolute intensity level and application of a cutoff value to accurately represent low density bony tissue HUs. For soft tissues, such as fat and muscle, located outside of the bone contours, a threshold-based segmentation method without requirements for any patient-specific adjustments was introduced to convert MRI intensity values into HUs. The dual model HU conversion technique was implemented by constructing pseudo-CT images for 10 other prostate cancer patients. The feasibility of these images for RTP was evaluated by comparing HUs in the generated pseudo-CT images with those in standard CT images, and by determining deviations in MRI-based dose distributions compared to those in CT images with 7-field intensity modulated radiation therapy (IMRT) with the anisotropic analytical algorithm and 360° volumetric-modulated arc therapy (VMAT) with the Voxel Monte Carlo algorithm. Results: The average HU differences between the constructed pseudo-CT images and standard CT images of each

  9. 麻核桃资源保护与开发利用研究%Conservation, Exploration and Utilization of Juglan hebeinesis Hu Resources

    Institute of Scientific and Technical Information of China (English)

    裴东; 李容海; 刘兆发; 王富强

    2006-01-01

    麻核桃(juglans hopeiensis Hu)不仅是核桃属植物中分布范围最窄,数量最少的一个种,而且是我国特有的种,但是多年来没有引起足够重视,研究报道很少,本文对麻核桃资源和生产情况进行了初步调查,并根据坚果外观变化鉴定出11个类型,目的是为资源保护和良种选育奠定基础.

  10. 沙伯基础创新塑料高强度Ultem HU1004树脂具备多重灭菌处理能力

    Institute of Scientific and Technical Information of China (English)

    Grace Tu

    2011-01-01

    最近,沙伯基础创新塑料公布第三方测试结果,证实超强UltemHU1004聚醚酰亚胺(PEI)树脂具备多重灭菌处理能力。该高性能树脂之前一直应用于其他技术,如今测试证实可在STERRADNX誖过氧化氢等离子低温灭菌过程中使用,让医疗保健供应商可以灵活选择托盘、电子医疗设备和其他设备的主要灭菌方式。

  11. 具有多重灭菌处理能力的高强度Ultem HU1004树脂

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    多重灭菌处理能力经沙伯基础创新塑料公布的第三方测试结果证实,超强Ultem HU1004聚醚酰亚胺(PEI)树脂具备多重灭菌处理能力。也就是说,该高性能树脂可在STERRAD NX 过氧化氢等离子低温灭菌过程中使用,使医疗保健供应商可以灵活选择托盘、医疗电子设备和其他医用设备的主要灭菌方式。

  12. Mössbauer Study of Ceramic Finds from the Galería de las Ofrendas, Chavín de Huántar

    Science.gov (United States)

    Lumbreras, L. G.; Gebhard, R.; Häusler, W.; Kauffmann-Doig, F.; Riederer, J.; Sieben, G.; Wagner, U.

    2003-09-01

    Ceramic finds from the Galería de las Ofrendas at Chavín de Huántar and surface finds from the settlement of Chavín were characterised by combining the results of archaeological typology with archaeometric studies using neutron activation analysis, Mössbauer spectroscopy, X-ray diffraction and thin-section microscopy. Sherds from the pyramid Tello are included in the study as representative of local material. The analyses show that the vessels were made from different raw materials and that different firing procedures were used in their production. Sherds of certain styles largely exhibit similar types of Mössbauer patterns and in many instances also have similar element compositions. This supports the archaeological notion that the vessels were brought to Chavín from the provinces, perhaps on the occasion of a festivity.

  13. Evaluación del riesgo sísmico del centro histórico de la Ciudad de Huánuco

    OpenAIRE

    Marín Guillen, Félix

    2012-01-01

    Las ciudades ubicadas en áreas de peligrosidad sísmica moderada o baja tienden a menospreciar el peligro sísmico y a descuidar precauciones básicas de protección frente a los terremotos, conllevando a un aumento de su vulnerabilidad y en consecuencia elevar el riesgo sísmico, que a su vez aumenta con el tiempo. En el presente estudio se obtuvieron escenarios de daño para el centro histórico de la ciudad de Huánuco, mediante una aproximación que posibilita y efectúa un análisis edificio a e...

  14. Priming with r-metHuSCF and filgrastim or chemotherapy and filgrastim in patients with malignant lymphomas: a randomized phase II pilot study of mobilization and engraftment

    DEFF Research Database (Denmark)

    Johnsen, Hanne; Geisler, C; Juvonen, E;

    2011-01-01

    methionyl human G-CSF (filgrastim, r-metHuG-CSF) (experimental arm A) or routine chemotherapy plus filgrastim (conventional arm B). The primary objective was to evaluate the side effects and toxicity during priming and mobilization. The secondary objectives were efficacy by the level of blood......-circulating PBPCs, the number of harvest days and the time to three-lineage engraftment after autografting. First, during priming 5 patients had 8 serious events, 4 in each arm. A summary of all adverse events revealed 30 (94%) patients suffering from 132 events of all grading. Second, neutropenia...... and thrombocytopenia was documented in arm B. Third, 9/14 (64%) patients in arm A reached the target of 5 million CD34(+) cells/kg body weight (bw) compared with 13/15 (87%) in arm B. The results represent the first randomized trial of growth factor plus chemotherapy priming and indicate that a formal phase III trial...

  15. Ultrasound-assisted extraction methodology as a tool to improve the antioxidant properties of herbal drug Xiao-chia-hu-tang.

    Science.gov (United States)

    Liu, Chu-Ting; Wu, Ching-Yi; Weng, Yih-Ming; Tseng, Chin-Yin

    2005-06-03

    Xiao-chai-hu-tang (XCHT) is an important Chinese herbal prescription for curing many types of liver diseases. The contents of bioactive constituents (saikosaponins a, c and d, baicalin, baicalein, and glycyrrhizic acid), and antioxidant properties of XCHT extracts prepared with ultrasound-assisted (US) extraction in combination with ethanol (up to 95%) as extraction modifier were studied. The results showed that the US extraction significantly increased the bioactive constituents concentrations and antioxidant properties of XCHT extracts when compared with the XCHT prepared with traditional boiling-water extraction. Among the XCHT extracts made with US extraction, the sample prepared with 95% ethanol showed the highest bioactive constituent concentrations and the best antioxidant functionalities. The results suggest that US extraction of XCHT is feasible to replace the traditional time-consuming and low efficiency preparation procedure in the future modernized and commercialized manufacture of this highly valuable Chinese herbal medicine.

  16. Communication Memoir between Hu Yaobang and Mao Zedong%胡耀邦与毛泽东交往实录(下)

    Institute of Scientific and Technical Information of China (English)

    李佑新; 吴璇

    2016-01-01

    胡耀邦是久经考验的忠诚的共产主义战士,伟大的无产阶级革命家、政治家,我军杰出的政治工作者,长期担任党的重要领导职务的卓越领导人。从20世纪30年代在陕北初识毛泽东,历经难忘的抗大时光和革命岁月,到新中国成立后主政川北、投身青年团工作、整顿中国科学院,胡耀邦得到了毛泽东的关怀、教育和培养,并始终追随毛泽东的事业。%Hu Yaobang was a long-tested loyal communist fighter,a great proletarian revolutionist,a statesman and an outstanding political worker of the people’s army,a brilliant leader who held the important post of the Party for a long time.From the 1930’s in the Northern Shaanxi he met Mao Zedong,and passed an unforgettable time in the Anti-Japanese military and political college and revolution years,to head the admin-istration in the Northern Sichuan Province,devote himself to the Communist Youth League and rectify the Chi-nese Academy of Sciences after the founding of new China,Hu Yaobang had been taken care of,educated and cultivated by Mao Zedong.He followed Mao Zedong’s career all his time.

  17. 胡适对五四运动的认识与思考%Hu Shi’ s Understanding and Thinking on the May 4th Movement

    Institute of Scientific and Technical Information of China (English)

    周良发

    2015-01-01

    胡适对五四运动的态度,前后有明显变化,由起初赞扬继而深表同情,及至晚年的批评。胡适认为五四运动的爆发除了中国政府的外交失败,还与北京高校学生庆祝一战胜利的游行活动有关,而五四运动的价值在于独立人格的形成,并成为社会发展的主要推力。胡适没有系统研究五四运动,但其言说仍对后世学人的形上致思颇多启发意义。%Hu Shi’ s attitude had a significant change about the May 4th Movement.He praised and showed sympathy in his early life, but later he harshly criticized.He thought that the May 4th outbreak was due to Chinese government diplomatic failure, and it’ s related to Peking university students to celebrate the end of the First World War.The May 4th value lied in the formation of the independent personality, and became the main thrust of social development.Hu Shi didn’ t study May 4th Movement systemical-ly, but what he said had a lot of inspiration significance for the later scholars.

  18. Analysis of the kinetic of expression of tristetraprolin and HuR by rheumatoid arthritis patients pheripheral blood mononuclear cells stimulated with lipopolysaccharide

    Directory of Open Access Journals (Sweden)

    G. Ferraccioli

    2011-09-01

    Full Text Available Objective. Given the role of TNF-α in Rheumatoid Arthritis (RA we decided to define the characteristics of the TNF- α synthesis by peripheral blood mononuclear cells (PBMNCs obtained from active-aggressive RA patients giving a particular attention to the modulation of the expression of two fundamental proteins in TNF-α mRNA stability regulation, Tristetraprolin (TTP and HuR. Methods. 11 RA patients with active disease were enrolled in the study before their entry in 2 double blind protocols: Infliximab versus MTX and Etanercept versus MTX. 9 healthy blood donors were taken as controls. PBMNCs obtained by Ficoll centrifugation and plastic adherence were stimulated with lipopolysaccharide (LPS and TNF-α was measured in the supernatant during 8 hours by ELISA. At each time point the cells were harvested and analysed for TNF- α, TTP and HuR mRNA expression by semi-quantitative PCR. Results. MNCs TNF-α secretion after LPS stimulation did not differ significantly between RA and control subjects, even if a tendency towards a more prompt response was observed in the patients. More importantly only the DMARDs responsive patients (DAS <3.7 at the 6th month, with a minimal reduction of 1.2 points disclosed precociously (at the first month a significant change in the profile of TNF-α secretion and maintained it until the 6th month. The “normalization” of the synthetic behaviour was accompanied by the resetting in the regulation of the expression of the TTP, that appeared significantly different in the patients before and after therapy. Conclusions. Independently from the type of therapy, responsive patients demonstrate a rapid change in the cellular biology at the systemic level that might drive the resolution of the phlogistic process at the synovial level.

  19. 基于中值滤波和Hu矩向量的手语识别%Sign language recognition based on Hu invariant moments and euclidean distance

    Institute of Scientific and Technical Information of China (English)

    华斌; 夏利娜

    2011-01-01

    To increase accuracy rate of sign language recognition, a recognition method based on Hu invariant moments and euclidean distance of sign language image is introduced. Firstly, its 7Hu moments are calculated, the recognition of 30 letter gestures is performed by computing distance between matching and template image to recognize image. The algorithm remains constant when the image rotates,transfers and scales. The method overcomes the impact of illumination in capturing image and marginal information loss caused by image segmentation. The experiments show the validity of this method especitaly a successful result of images with a high rate of pepper-andsalt noise.%为提高手语图像的正识率,提出一种基于中值滤波和Hu不变矩特征量相结合的手语识别方法,对待匹配图像进行预处理,计算图像的7Hu矩特征量,通过欧氏距离计算与标准手语特征向量的相似程度,识别手语,对旋转、缩放和平移等图像的非本质性改变具有很好的鲁棒性,克服了手语图像采集中光照不均的影响和传统手语识别中基于图像分割造成的边缘信息损失.实验结果表明了该方法对手语识别的有效性,特别是对含有椒盐噪声信噪比较高的图像具有很好的效果.

  20. Internal Causes and Reflections on Wretchedness of Female Existence--Comment on Feng Shui by Fang Fang%世俗环境中的痛楚阴影与美丽光芒——影片万箭穿心的女性主义阐释

    Institute of Scientific and Technical Information of China (English)

    李静

    2012-01-01

    Based on an ordinary female worker Li Baoli's marriage tragedy, Fang Fang describes her suffering, reveals the internal causes of wretchedness of modem female existence, and reflects how women maintain self-value and dignity in their marriages and family lives in her novel Feng Shui.%方方《万箭穿心》围绕普通女工李宝莉的婚姻悲剧,道尽了其处于生活中的种种痛苦。作者以冷峻的笔调揭露了现代女性生存之痛的内在原因,同时也反思女性在婚姻家庭生活中如何保持自我价值和尊严。

  1. The Value of Western Yunnan Anti-Japanese Novels--A Case Study of the Jian Sao Feng Yan written by the peasant writer, Duan Peidong%数年铸一卷剑扫风烟直--滇西抗战小说《剑扫风烟》艺术价值探析

    Institute of Scientific and Technical Information of China (English)

    刘蜀子

    2016-01-01

    《剑扫风烟》是农民作家段培东滇西抗战三部曲的第一部长篇纪实小说,全书三十余万字,以简明的结构,质朴的语言,栩栩如生的人物群象,生动的细节描写向我们勾勒了当年腾冲沦陷,人民浴血奋战的悲壮景象。%The Jian Sao Feng Yan written by the peasant writer, Duan Peidong, is the first one of his western Yunnan anti-Japanese documentary trilogy. The 300 thousand words novel lively depicts the history of the brave people fighting the Japanese invaders in Tengchong with simple structure, plain language and vivid figures.

  2. A neonate with the Pelger-Huët anomaly, cleft lip and palate, and agenesis of the corpus callosum, with a chromosomal microdeletion involving 1q41 to 1q42.12.

    Science.gov (United States)

    Christensen, R D; Yaish, H M

    2012-03-01

    We observed a neonate with cleft lip and palate, 13 sets of ribs, agenesis of the corpus callosum, slightly small penis, hypoglycemia, and what initially appeared to be a marked leukocyte 'left shift' on complete blood count, but which was actually a Pelger-Huët anomaly. A chromosomal microdeletion was identified at1q41-42.12.

  3. Phase I/II clinical and pharmacokinetic study evaluating a fully human monoclonal antibody against EGFr (HuMax-EGFr) in patients with advanced squamous cell carcinoma of the head and neck

    DEFF Research Database (Denmark)

    Bastholt, Lars; Specht, Lena; Jensen, Kenneth;

    2007-01-01

    reactions varied from few days to 2 months. No DLTs were observed and MTD was not reached. In the two highest dose groups, 7 of 11 patients obtained a PR or SD and 9 patients obtained metabolic PR or SD. CONCLUSIONS: HuMax-EGFr can be safely administered in doses up to 8 mg/kg, and preliminary data...

  4. Research Upregulation of CD23 (FcεRII Expression in Human Airway Smooth Muscle Cells (huASMC in Response to IL-4, GM-CSF, and IL-4/GM-CSF

    Directory of Open Access Journals (Sweden)

    Lew D Betty

    2005-05-01

    Full Text Available Abstract Background Airway smooth muscle cells play a key role in remodeling that contributes to airway hyperreactivity. Airway smooth muscle remodeling includes hypertrophy and hyperplasia. It has been previously shown that the expression of CD23 on ASMC in rabbits can be induced by the IgE component of the atopic serum. We examined if other components of atopic serum are capable of inducing CD23 expression independent of IgE. Methods Serum starved huASMC were stimulated with either IL-4, GM-CSF, IL-13, IL-5, PGD2, LTD4, tryptase or a combination of IL-4, IL-5, IL-13 each with GM-CSF for a period of 24 h. CD23 expression was analyzed by flow cytometry, western blot, and indirect immunofluorescence. Results The CD23 protein expression was upregulated in huASMC in response to IL-4, GM-CSF, and IL-4/GM-CSF. The percentage of cells with increased fluorescence intensity above the control was 25.1 ± 4.2% (IL-4, 15.6 ± 2.7% (GM-CSF and 32.9 ± 13.9% (IL-4/GMCSF combination(n = 3. The protein content of IL-4/GMCSF stimulated cells was significantly elevated. Expression of CD23 in response to IL-4, GM-CSF, IL-4/GM-CSF was accompanied by changes in cell morphology including depolymerization of isoactin fibers, cell spreading, and membrane ruffling. Western blot revealed abundant expression of the IL-4Rα and a low level expression of IL-2Rγc in huASMC. Stimulation with IL-4 resulted in the phosphorylation of STAT-6 and an increase in the expression of the IL-2Rγc. Conclusion CD23 on huASMC is upregulated by IL-4, GM-CSF, and IL-4/GM-CSF. The expression of CD23 is accompanied by an increase in cell volume and an increase in protein content per cell, suggesting hypertrophy. Upregulation of CD23 by IL-4/GM-CSF results in phenotypic changes in huASMC that could play a role in cell migration or a change in the synthetic function of the cells. Upregulation of CD23 in huASMC by IL-4 and GM-CSF can contribute to changes in huASMC and may provide an avenue

  5. Expression and Purification of Functional HuGALNT3 without the Transmembrane Domain (huGALNT3-sol) in Pichia pastoris%重组人GALNT3-sol蛋白在毕赤酵母中的高效表达和活性鉴定

    Institute of Scientific and Technical Information of China (English)

    孔蕴; 郜海涛; 李树芳; 王鹏; 顾国锋; 顾黎

    2012-01-01

    目的:为了获得有催化活性的人乙酰半乳糖胺转移酶3(GALNT3),构建了GALNT3可溶性区域(GALNT3-sol)的真核分泌表达载体,在巴斯德毕赤酵母中表达并纯化GALNT3-sol蛋白,体外检测其转糖基活性.方法:以构建好的pET15b/GALNT3-sol为模板进行PCR,扩增编码人GALNT3-sol的cDNA片段(1755 bp),将其克隆至真核表达载体pPIC9K,载体线性化后采用电击法转化毕赤酵母GS115.通过MD平板和G418平板筛选出阳性高拷贝重组菌株.阳性菌株经过甲醇诱导表达人GALNT3-sol重组蛋白,表达上清进行Ni-NAT分离纯化.分别采用SDS-PAGE和Western blot鉴定纯化的重组蛋白,并使用HPLC和MALDI-TOF/MS分析其转糖基化反应的活性.结果:成功构建了能够分泌表达GALNT3-sol的毕赤酵母菌株.阳性表达菌株在BMMY培养基(pH 6.0)中20℃培养,经0.5%甲醇诱导表达96 h,摇瓶表达量可达5mg/L.SDS-PAGE和Western blot结果显示表达重组蛋白为糖基化形式.活性检测显示表达的重组蛋白具有转糖基活性.结论:成功获得可以高效分泌表达具有活性的人GALNT3-sol蛋白的毕赤酵母菌株,为进一步研究人GALNT3的性质及其应用提供了基础.%Objective: In order to research the bioactivity of GALNT3, the truncated part of GALNT3 (huGALNT3-sol) which was deleted of the hydrophobic trans-membrane domain were obtained using Pichia pastoris expression system, and assayed the transferring GalNAc activity of recombinant huGALNT3-sol. Methods; The gene of human GALNT3-sol (1 755 bp)was amplified from pET15b/ GALNTi-sol and cloned into expression vector pPIC9k, and the recombinant plasmid was transformed into Pichia pastoris GS115 strain through electroporation. The high copy recombinant strains with high-level huGALNT3-sol production were screened out by MD plate and G418. High level of huGALNT3-sol was obtained in BMMY medium the induction of methanol, and purified from the supernatant with Ni-NAT. The identity of

  6. HuD蛋白对肛门直肠畸形子鼠直肠末端肠神经系统的影响%The impact of the HuD protein on the intestinal nervous system in the rectal end of animal models of congenital anorectal malformation

    Institute of Scientific and Technical Information of China (English)

    孔萌; 刘远梅; 金祝; 高明娟; 曲颜; 郑泽兵

    2016-01-01

    Objective To investigate the expression of the HuD protein in the rectal end of anorectal malformation (ARM) embryonic rats and to explore the effect of HuD expression on the development of the intestinal nervous system.Methods 20 adult pregnant Sprague Dawley rats were randomized into a control (n =10) and ARM (induced by ethylenethiourea) group (n =10).On the 20th day after pregnancy,the cesarean section fetus were obtained for morphological observations and abnormal statistics.Using hematoxylin-eosin (HE) staining to detect the perineum of sagittal gross specimen.Using immunohistochemistry (IHC) and Western blotting to detect the protein expression levels of HuD of distal rectum in fetal rats.Results The normal control group was given birth to 108 fetal rats whose survival rate was 100.0% and no rectal abnormal openings,the ARM group was given birth to 94 fetal rats whose survival rate was 93.6% and ARM rate was 96.8%.In HE staining,there was no opening at the end of the distal rectum of the ARM fetuses,the end of the rectum had thickened muscular layer and covered by hyperplastic squamous epithelial without intestinal gland distribution.The number of submucosal and myenteric plexus alse reduced significantly in ARM fetuses compared with controls (143.60 ± 14.93 vs.335.90 ± 10.46,P <0.01).The immunohistochemistry and Western blotting analysis showed that the concentration of the HuD protein in the ARM group was significantly lower than that in the control group (312.90 ± 53.40 vs.456.40 ± 57.13;0.24 ± 0.05 vs.0.45 ± 0.06,P < 0.01).Conclusion The HuD protein was abnormally expressed in the rectal end of the ARM embryonic rats and may participate in the development and maturation of the enteric nervous system (ENS).This study may provide a useful theoretical reference for the treatment of postoperative defecation dysfunction in ARM patients.%目的 检测HuD蛋白在肛门直肠畸形(ARM)子鼠直肠末端组织中的表达,探讨其可能对肠

  7. Historical Responsibility Thought of Intergenerational Climate Justice:Discussion with Dr. XU Feng%代际气候正义中的历史责任沉思--兼与徐峰博士商榷

    Institute of Scientific and Technical Information of China (English)

    叶方兴

    2014-01-01

    The formation of climate change is not only a specific social and historical process, and also a process of constantly from local to global. As a product of modern industrial civilization, the issue of climate change is the consequence of modernity. Intergenerational climate justice deals with the relationship between in-tergenerational justice on climate resource allocation and consumption, mainly includes the former generations should bear what kind of historical responsibility and contemporary people have what kind of obligation for the future. In identification of the historical responsibility of Intergenerational climate justice, Dr. Xu Feng proposed“pardonable ignorance” can not disclaim for atmospheric pollution behavior of developed countries or the previ-ous generation�s exemption. The basis of historical responsibility in the developed countries is “reasonable care”. Human beings must conduct social cooperation under the condition of limited resources, and the “rea-sonable care” is able to guarantee the smooth realization of the social cooperation and social development;Cli-mate change is the important embodiment of modernity risk, “reasonable care” is an effective idea to address climate change. Imputation foothold on climate change is that the parties to the main body of the behavior, it is the main body failed to fulfill their obligation to“reasonable care” and has caused the global greenhouse effect.%气候变化问题的生成既是一个具体的社会历史进程,也是一个不断地由地方性转向全球性的过程。作为现代工业文明的产物,气候变化问题是现代性的后果。代际气候正义处理的是代际之间在气候资源配置与消费上的正义关系,主要包括前代人应该承担什么样的历史责任和当代人对未来人具有什么样的义务。在代际气候正义的历史责任认定上,“可原谅的无知”不能为发达国家或前代人的大气污染行为免

  8. Glycosylphosphatidylinositol-Anchored Anti-HIV scFv Efficiently Protects CD4 T Cells from HIV-1 Infection and Deletion in hu-PBL Mice.

    Science.gov (United States)

    Ye, Chaobaihui; Wang, Weiming; Cheng, Liang; Li, Guangming; Wen, Michael; Wang, Qi; Zhang, Qing; Li, Dan; Zhou, Paul; Su, Lishan

    2017-02-01

    Despite success in viral inhibition and CD4 T cell recovery by highly active antiretroviral treatment (HAART), HIV-1 is still not curable due to the persistence of the HIV-1 reservoir during treatment. One patient with acute myeloid leukemia who received allogeneic hematopoietic stem cell transplantation from a homozygous CCR5 Δ32 donor has had no detectable viremia for 9 years after HAART cessation. This case has inspired a field of HIV-1 cure research focusing on engineering HIV-1 resistance in permissive cells. Here, we employed a glycosylphosphatidylinositol (GPI)-scFv X5 approach to confer resistance of human primary CD4 T cells to HIV-1. We showed that primary CD4 T cells expressing GPI-scFv X5 were resistant to CCR5 (R5)-, CXCR4 (X4)-, and dual-tropic HIV-1 and had a survival advantage compared to control cells ex vivo In a hu-PBL mouse study, GPI-scFv X5-transduced CD4 T cells were selected in peripheral blood and lymphoid tissues upon HIV-1 infection. Finally, GPI-scFv X5-transduced CD4 T cells, after being cotransfused with HIV-infected cells, showed significantly reduced viral loads and viral RNA copy numbers relative to CD4 cells in hu-PBL mice compared to mice with GPI-scFv AB65-transduced CD4 T cells. We conclude that GPI-scFv X5-modified CD4 T cells could potentially be used as a genetic intervention against both R5- and X4-tropic HIV-1 infections. Blocking of HIV-1 entry is one of most promising approaches for therapy. Genetic disruption of the HIV-1 coreceptor CCR5 by nucleases in T cells is under 2 clinical trials and leads to reduced viremia in patients. However, the emergence of viruses using the CXCR4 coreceptor is a concern for therapies applying single-coreceptor disruption. Here, we report that HIV-1-permissive CD4 T cells engineered with GPI-scFv X5 are resistant to R5-, X4-, or dual-tropic virus infection ex vivo In a preclinical study using hu-PBL mice, we show that CD4 T cells were protected and that GPI-scFv X5-transduced cells were

  9. Los orígenes del Colegio Menor de la Concepción de Huérfanos de Salamanca

    Directory of Open Access Journals (Sweden)

    Miguel A. MARTÍN SÁNCHEZ

    2009-12-01

    Full Text Available RESUMEN: El Colegio Menor de la Concepción de Huérfanos de la Universidad de Salamanca es uno más de la gran cantidad de colegios que se fundaron al amparo del estudio salmantino en el siglo XVI. Fundado por Francisco de Solís, obispo de Bagnorea y secretario de Paulo III, durante tres siglos facilitó y proporcionó amparo y educación a multitud de estudiantes pobres que no tenían recursos para ello. Fundado en la primera mitad del siglo XVI, durante esta centuria se asentó y comenzó su andadura, que terminaría en el siglo XIX. Este trabajo realiza un análisis sobre los primeros años de vida del Colegio, analizando cuestiones tan importantes como su fundación, las bulas fundacionales, el fundador, las primeras constituciones o la matrícula durante el siglo XVI, entre otras. Fue el siglo XVI el siglo de su nacimiento y consolidación, realizando durante esta centuria un gran esfuerzo institucional, como demuestra el hecho de la elaboración de las primeras constituciones, la construcción del edificio, o la incorporación a la Universidad de Salamanca, por ejemplo. A lo largo de estas páginas realizaremos un recorrido por los orígenes del primer colegio universitario que se crea para el amparo de niños pobres y huérfanos, permitiéndoles estudiar desde la gramática hasta graduarse en la ciencia que mejor les pareciere. La obra social y educativa del Colegio permitió la progresión y ascenso social de multitud de estudiantes pobres y huérfanos con talento, que de otra forma nunca hubieran podido realizar estudios en la Universidad, puesto que carecían de recursos para ello.ABSTRACT: The Conception of Orphans Minor College of the University of Salamanca is one more of the great quantity of colleges that were founded under protection of Salamancan Study in the 16th century. It was founded by Francisco de Solís, bishop of Bagnorea and secretary of Paulo III, provided protection and education to many poor students without

  10. Internação domiciliar: o perfil dos pacientes assistidos pelo Programa HU em Casa Home care: profile of patients attended by a home care program

    Directory of Open Access Journals (Sweden)

    Daniella Reis Barbosa Martelli

    2011-01-01

    Full Text Available Internação domiciliar é uma modalidade de atendimento à saúde que está se transformando em uma alternativa importante para minimizar alguns dos principais problemas inerentes aos sistemas de saúde vigentes, especialmente os da rede pública. O objetivo do estudo foi descrever o perfil sociodemográfico e clínico da população assistida pelo Programa de Internação Domiciliar (PID HU em Casa do Hospital Universitário Clemente de Faria da Universidade Estadual de Montes Claros. O estudo foi descritivo e retrospectivo por meio da análise de prontuários, realizada de maio de 2005 a maio de 2008. Foram analisados 137 pacientes, sendo 75 do gênero feminino (54,7% e 62 do masculino (45,3%. O grupo de 61 a 80 anos foi mais prevalente (37,2% e 73% dos pacientes residiam em bairros periféricos do município de Montes Claros-MG. Dos agravos mais comuns na primeira internação, a pneumonia foi prevalente, 22 casos (16,1%. A maioria dos pacientes foi encaminhada ao PID pela clinica médica (84,7%, com intervalo de maior prevalência de duas a três internações (42,4%. Do total de pacientes, 120 (87,6% permaneceram internados por 16 a 30 dias e 51,8% não necessitaram passar novamente pelo PID para uma segunda internação. Com relação à resolutividade clínica, 130 (94,9% tiveram alta clínica, no PID, na primeira internação. O PID mostrou-se ser um programa de alta resolutividade, atendendo mais ao público idoso feminino, de baixa renda e com períodos de internação relativamente curtos.The home care is a modality of health care which is becoming an important alternative to minimize some of mainly relevant problems of world health, especially the public health network. This paper aimed to describe the socio-demographic and clinical population assisted by the Home Care Program HU em Casa, of the University Hospital Clemente de Faria, Universidade Estadual de Montes Claros. It is a descriptive and retrospective study analyzing

  11. La masacre de Ambo: Histoire d'une revolte paysanne, département de Huánuco, Pérou, 1962-1963

    Directory of Open Access Journals (Sweden)

    2004-01-01

    Full Text Available À travers l’histoire de la révolte de la communauté de Huaylla y Aclacancha (vallée d’Ambo, département de Huánuco il est possible de comprendre comment s’organisent les paysans comuneros pour défendre leurs terres communautaires. L’analyse des requêtes paysannes envoyées aux autorités gouvernementales dévoile l’importance pour la communauté de faire valoir la légitimité de ses droits de propriété, en s’appuyant à la fois sur des actions légales et sur des occupations de terre. Cependant l’utilisation simultanée de ces moyens de revendication n’explique pas en soi le dérapage vers la violence, connu dans la presse comme la masacre de Ambo (la tuerie d’Ambo. Seule une étude des contextes dans lesquels interagissent tous les acteurs concernés (comuneros, syndicalistes, autorités politiques, policiers et hacendados peut permettre de saisir pourquoi les réclamations de cette communauté ont débouché sur la mort de comuneros. A través la historia de la lucha de la comunidad de Huaylla y Aclacancha (provincia de Ambo, departamento de Huánuco se puede comprender cómo se organizan los comuneros para defender sus tierras comunales. El análisis de las solicitudes enviadas por los comuneros a las autoridades gubernamentales nos muestra la importancia, para la comunidad, de hacer valer tanto la legitimidad de sus derechos de propiedad como, al mismo tiempo, apoyarse sobre acciones legales y ocupaciones de tierras. La utilización simultánea de esos medios de reivindicación no explica en sí el deslizamiento violento, conocido en la prensa como la masacre de Ambo. Pero sí, el análisis de los contextos en los cuales interactuan todos los actores de ese drama (comuneros, sindicalistas, autoridades políticas, gardias civiles y hacendados nos permite comprender por qué las reclamaciones de esta comunidad desembocan en la muerte de comuneros. Through a history of the community’s rebellion (Huaylla y Aclacancha, in

  12. La estrategia diplomática-militar de España reflejada en la correspondencia extraoficial del Duque de Huéscar entre 1747 y 1748

    Directory of Open Access Journals (Sweden)

    Naiara Pavía Dopazo

    2014-02-01

    Full Text Available En este artículo se pretende contribuir al conocimiento de los sucesos bélicos acaecidos en Génovadurante la primavera de 1747, a través del intercambio de correspondencia inédita entre algunos delos principales aliados españoles de esta república, capitaneados por Carvajal y Ensenada que encontraronen don Fernando de Silva, duque de Huéscar (luego de Alba, el mejor enlace para la conformaciónde una red social que permitiera un conocimiento extraoficial que mejorara la capacidad deactuación a favor de los intereses de España en dicha república. Un análisis interpersonal de un hechohistórico que pone de manifiesto la «tensa relación» diplomática entre Francia y España, unidas porel Pacto de Familia de 1743 y que determinó las estrategias diplomáticas llevadas por ambos durantelas negociaciones de la paz de Aquisgrán, que puso fin a la Guerra de Sucesión Austriaca.With this article we’re trying to contribute to the knowledge of the warlike events occurred in Genoaduring the spring of 1747, through the exchange of unknown correspondence among some of theprincipal Spanish allies of this republic, led by Carvajal and Ensenada who found in don Fernando ofSilva, Duke of Huéscar (later Duke of Alba, the best link for the conformation of a social network thatallowed an unofficial knowledge in order to improve the capacity of action in favour of the interestsof Spain in the above mentioned republic. An interpersonal analysis of a historical fact that revealsthe diplomatic «tense relationship” between France and Spain, joined by the Family agreement of 1743and that was decisive for the diplomatic strategies accomplished by both of them during thenegotiations of the Peace of Aachen (Aix-la-Chapelle, which came to an end with the War of theAustrian Succession.

  13. On Contributions of Hu Shi’s Reading Lvshi Chunqiu%胡适《读吕氏春秋》的贡献

    Institute of Scientific and Technical Information of China (English)

    王启才

    2015-01-01

    Reading Lvshi Chunqiu,collected in Collections ofHu Shi Essays III (vol.3 ),is a research paper on Lvshi Chunqiu written by Hu Shi in 1930.Shifting from the traditional emphasis on textual interpretation,the paper adopted a cont-rastive perspective,focusing on human desire and nature,and proposed quite a few valuable points of view.Based on histori-cal evidence,the paper took a broad scope of vision in writing,its writing style and manner were easy to accept,and its meth-od and conclusion were fresh and new.The paper preliminarily changed the roadmap of later research on Lvshi Chunqiu and became the pioneering work in the modern history of Lvshi Chunqiu research.%写于1930年,后收入《胡适文存三集》卷三的《读吕氏春秋》,是胡适的一篇研究《吕氏春秋》的论文。该文打破了有汉以来研究《吕氏春秋》只重视文本解释的局限,采用了中西比较的思路与方法,从人欲、人性的思想高度审视把握《吕氏春秋》,提出了不少现在看来仍不失为有价值的观点与问题。文章视野开阔,论从史出,文风明白晓畅,易于接受,方法与结论都令人耳目一新,初步改变了以后《吕氏春秋》研究的路向,在近代《吕氏春秋》研究史上具有开拓之功。

  14. 直接分子克隆法构建重组腺病毒AdHu5-mfsp27的研究%Direct molecular cloning to construct recombinant AdHu5-mfsp27 and its amplification and purification

    Institute of Scientific and Technical Information of China (English)

    王群; 贾蓓; 魏晓宇; 黄文祥; 周向阳

    2012-01-01

    Objective The aim of this study was to construct recombinant AdHu5-040-mfsp27 (cds) using direct molecular cloning and obtain viruses with a high titer and level of purity for laboratory and clinical trials. Methods The target gene mfsp27 was cloned into pShuttle and located between two rare restriction enzymes (REs) (I-ceuI and PI-sceI). After digestion, gel ligation, and transformation, recombinants were selected and confirmed by specific RE cutting and sequencing. The candidate recombinant plasmid was then linearized and transfected into HEK293 cells. After infection and expansion, the crude virus stock was collected and purified by modified cesium chloride gradient centrifugation. The virus multiplicity of infection (MOD was measured by series dilution of the stock. Results Recombinant AdHu5-040-fsp27 (cds) was successfully constructed. The titer (O. D. measurement) was as high as to 1 × 1013 VP/ ml and MOI was 1. 44 × 1011. The ratio of the two values was 69. 44. The recombinant virus had potent ability to infect HEK293 cells. Conclusion Direct molecular cloning to construct recombinant Ad Hu5-040-fsp27 (cds) is better than homologous recombination because it takes less time and it provides highly positive clones with fewer mutations of target genes. Modified cesium chloride gradient centrifugation combined with a liquid chromatography column and biogels is a simple and efficient way to obtain purified viruses in high tilers and with a high level of activity. This provides an experimental basis for the study of gene function and the industrial production of recombinant adenovirus.%目的 采用直接分子克隆体外连接的方法构建重组腺病毒AdHu5-040-mfsp27(cds),高效高质量扩增和纯化重组腺病毒以满足实验及临床研究的需求. 方法 将mfsp27克隆至穿梭质粒pSh CMV intron eGFP两个稀有限制性内切酶之间(I-ceuI和PI-sceI)(与腺病毒骨架质粒相同),回收酶切片段后连接,用合适的感受态细胞转

  15. 湖南名菜辣味特征及相关味型分析%Pungency Characteristics of Hu'nan Dishes and Analysis of the Related Taste Types

    Institute of Scientific and Technical Information of China (English)

    何帅; 杨帆; 叶小清; 谢定源

    2016-01-01

    Take the book TheHu'nanFlavorofChineseFamousRecipes as a statistical reference,the quantitative analysis of the pungency characteristics of Hu'nan dishes is carried out.Meanwhile,based on the reference and analysis of the spicy food's composition in the representative recipes and the spicy food's consumption per person in Hu'nan Province,synthesize the overall pungency characteristics of Hu'nan dishes.Use the HPLC method to analyze and detect the content of capsaicin and dihydrocapsaicin in seasonings.With the combination of the pungency degree calculation principle and recipes weight calculation principle,the pungency degree in all kinds of Hu'nan dishes is counted.The research shows that the average pungency degree of capsaicin in the dishes is 1.474°,while the average pungency degree of capsaicin in all dishes is 0.264°.In all Hu'nan spicy dishes,the largest proportion of taste type is “hot-and-sour”.%以《中国名菜谱·湖南风味》为统计参考,量化分析湖南名菜的辣味特征。同时,基于对湖南省代表性食谱中含辣食品的构成以及湖南省辣味食品人均消费情况的参考分析,综合归纳出湖南名菜辣味的整体特征。采用高效液相色谱法分析测定各调味料中辣椒素、二氢辣椒素的含量,并结合辣度计算和菜谱计重原则,统计出湖南各类名菜的辣度特征。研究表明:辣椒素类物质在所有添加含辣椒素类物质的菜肴中的平均辣度为1.474°,辣椒素类物质在所有菜肴中的平均辣度为0.264°。在湖南辣味菜肴中,“酸辣”味型所占比例最大。

  16. 琴叶风吹楠(肉豆蔻科)分类学位置再研究%Taxonomic Position of Horsfieldia pandurifolia Hu(Myristicaceae)

    Institute of Scientific and Technical Information of China (English)

    吴裕; 毛常丽; 张凤良; 杨晓玲; 曾建生; 段安安

    2015-01-01

    琴叶风吹楠(Horsfieldia pandurifolia Hu)自1963年命名发表以来,其分类学位置一直存在争议.本文以中国野生肉豆蔻科(Myristicaceae)11个种为材料,从形态学、油脂化学和分子遗传学方面对琴叶风吹楠分类学位置进行研究.结果表明,琴叶风吹楠与H.macrocoma ssp.prainii的形态特征高度一致,与中国风吹楠属其它类群,以及红光树属和肉豆蔻属植物在形态学和油脂化学方面存在决定性差别,在分子遗传学方面存在较大差异.支持将H.pandurifolia并入H.macrocoma ssp.prainii,以H.macrocoma为模式种建立Endocomia属,琴叶风吹楠的学名变更为Endocomia macrocoma(Miq.) de Wilde ssp.prainii(King) de Wilde.

  17. SupT1 Cell Infusion as a Possible Cell-Based Therapy for HIV: Results from a Pilot Study in Hu-PBMC BRGS Mice

    Directory of Open Access Journals (Sweden)

    Jonathan Fior

    2016-04-01

    Full Text Available In a previous in vitro study, the SupT1 cell line was explored as a decoy target for HIV-1, proposing SupT1 cell infusion as a possible cell-based therapy for HIV. In the present work, the previous in vitro model was translated into an in vivo setting. Specifically, Hu-PBMC BRGS mice were infected with a high input of HIV-1 LAI (100,000 TCID50, and 40 million 30 Gy-irradiated SupT1 cells were infused weekly for 4 weeks as a therapy. Blood samples were taken to monitor CD4+ T cell count and viral load, and mice were monitored daily for signs of illness. At the earliest time point analyzed (Week 1, there was a significantly lower plasma viral load (~10-fold in all animals treated with SupT1 cell infusion, associated with a higher CD4+ T cell count. At later time points, infection proceeded with robust viral replication and evident CD4+ T cell depletion, except in one mouse that showed complete suppression of viral replication and preservation of CD4+ T cell count. No morbidity or mortality was associated with SupT1 cell infusion. The interesting tendencies observed in the generated data suggest that this approach should be further investigated as a possible cell-based HIV therapy.

  18. Factores asociados a la infección por Ehrlichia canis en perros infestados con garrapatas en la ciudad de Huánuco, Perú

    Directory of Open Access Journals (Sweden)

    Edward Huerto-Medina

    Full Text Available El objetivo del estudio fue determinar la frecuencia y factores asociados a la infección por Ehrlichia canis en perros. Se recolectaron muestras de sangre de 150 perros infestados con garrapatas en 10 consultorios veterinarios de la ciudad de Huánuco en Perú, los perros fueron seleccionados al azar sin distinción de raza, edad ni sexo. Se detectó anticuerpos contra Ehrlichia canis mediante inmunoensayo cromatográfico. El 51,3% de perros estuvieron infectados por Ehrlichia canis. En el análisis multivariado se encontraron asociados a la presencia Ehrlichia canis, el mal estado de salud del perro (p=0,049, un promedio mayor de infestación por garrapatas (p=0,018, perros de edad adulta (p=0,038. La frecuencia de Ehrlichia canis en perros de esta ciudad es alta. Se recomienda el control de la garrapata marrón del perro (Rhipicephalus sanguineus vector de la Ehrlichia canis

  19. Hu Jintao's Publicity Ideas in New Era%试论胡锦涛新时期的宣传工作思想

    Institute of Scientific and Technical Information of China (English)

    梁友佳

    2011-01-01

    The importance of publicity is growing,awareness and advocacy work to solve the problems became one of the important issues faced by the party and the country.Comrade Hu Jintao based on the development of socialist modernization with Chinese characteristics,the new reality for the development of new international and domestic changes,explore the importance of advocacy work,guiding principles,system development and work management,and give a lot of useful and insightful answers.%宣传工作的重要性日益凸显,认识和解决宣传工作面临的问题成为党和国家工作的一大重要课题。胡锦涛同志立足于中国特色社会主义现代化建设发展的新实际,针对国际国内发展的新变化,对宣传工作的重要性、指导原则、制度建设以及工作管理等方面进行了许多有益的探索和精辟的回答。

  20. 从胡锦涛的青年观谈当代青年思想引导%On Youth Ideological Guidance from Hu Jintaos Views on Youth

    Institute of Scientific and Technical Information of China (English)

    任飞

    2012-01-01

    胡锦涛的青年观深刻洞悉当代中国社会和当代中国青年发展的内在机理和特点,特别强调用发展的眼光和全面的视野正确认识当代青年,培养青年成长成才。大学生、企业青年、农村青年以及进城务工青年这四类青年是当代青年的主要组成部分。有针对性地、分类地、具体地开展青年群体的思想引导工作,是胡锦涛青年观的重要内涵。%Hu Jintaog view on youth has a deep insight into the contemporary Chinese society and the inner mechanism and characteristics of contemporary Chinese youth development, with special emphasis on vision andat the cultivation of migrant youth explore thinkingguidance of youth groups in a specific, targeted, classified way.

  1. Direct versus indirect climate controls on Holocene diatom assemblages in a sub-tropical deep, alpine lake (Lugu Hu, Yunnan, SW China)

    Science.gov (United States)

    Wang, Qian; Yang, Xiangdong; Anderson, Nicholas John; Dong, Xuhui

    2016-07-01

    The reconstruction of Holocene environmental changes in lakes on the plateau region of southwest China provides an understanding of how these ecosystems may respond to climate change. Fossil diatom assemblages were investigated from an 11,000-year lake sediment core from a deep, alpine lake (Lugu Hu) in southwest China, an area strongly influenced by the southwest (or the Indian) summer monsoon. Changes in diatom assemblage composition, notably the abundance of the two dominant planktonic species, Cyclotella rhomboideo-elliptica and Cyclostephanos dubius, reflect the effects of climate variability on nutrient dynamics, mediated via thermal stratification (internal nutrient cycling) and catchment-vegetation processes. Statistical analyses of the climate-diatom interactions highlight the strong effect of changing orbitally-induced solar radiation during the Holocene, presumably via its effect on the lake's thermal budget. In a partial redundancy analysis, climate (solar insolation) and proxies reflecting catchment process (pollen percentages, C/N ratio) were the most important drivers of diatom ecological change, showing the strong effects of climate-catchment-vegetation interactions on lake functioning. This diatom record reflects long-term ontogeny of the lake-catchment ecosystem and suggests that climatic changes (both temperature and precipitation) impact lake ecology indirectly through shifts in thermal stratification and catchment nutrient exports.

  2. Serum metabonomics study of anti-depressive effect of Xiao-Chai-Hu-Tang on rat model of chronic unpredictable mild stress.

    Science.gov (United States)

    Xiong, Zhili; Yang, Jie; Huang, Yue; Zhang, Kuo; Bo, Yunhai; Lu, Xiumei; Su, Guangyue; Ma, Jie; Yang, Jingyu; Zhao, Longshan; Wu, Chunfu

    2016-09-01

    Xiao-Chai-Hu-Tang (XCHT) has been proven to be effective for the clinical treatment of depression. However, the mechanisms of definite antidepressant-like effects and detailed metabolic biomarkers were still unclear in this prior study. Here, we have investigated the metabolic profiles and potential biomarkers in a chronic unpredictable mild stress model after treatment with XCHT. Metabonomics based on ultra-high performance liquid chromatography coupled with mass spectrometry was used to profile the metabolic fingerprints of serum obtained from a rat model with chronic unpredictable mild stress with and without XCHT treatment. The model rats showed a significant decrease in sucrose preference and food consumption, and these depression-like symptoms were significantly improved by XCHT. Through principal component analysis (PCA), nine potential biomarkers of tryptophan, uric acid, phenylalanine, cholic acid and lysophosphatidylcholine (C18:0 LPC, C16:0 LPC, C16:1 LPC, C18:1 LPC, C20:4 LPC) were characterized as potential biomarkers involved the pathogenesis of depression. The therapeutic effect of XCHT on depression may involve in amino acid metabolism, lipid metabolism, oxidative stress and inflammation response. The present investigation highlights that metabonomics is a valuable tool for studying the essence of depression as well as evaluating the efficacy of the corresponding drug treatment.

  3. Contents of the heavy metals nickel and lead in leaves of Paulownia elongata S.Y. Hu and Paulownia fortunei Hems. in Serbia

    Directory of Open Access Journals (Sweden)

    Stanković Dragica

    2009-01-01

    Full Text Available A comparative study was carried out on the capabilities of the two species Paulownia elongata S. Y. Hu and Paulownia fortunei Hems. as biomonitors of atmospheric (and soil heavy-metal pollution. Research on content of the heavy metals nickel (Ni and lead (Pb in leaves of the species Paulownia elongata growing under urban and suburban conditions with the comparison to the concentration of these elements in leaves of the species Paulownia elongata and Paulownia fortunei on an experimental plot in Bela Crkva showed average nickel concentrations of 2.7 μg/g on the experimental plot in Bela Crkva, Serbia, twice as low as the concentrations measured under extreme urban conditions (6.62 μg/g, or 4.54 μg/g in the immediate vicinity of suburban traffic lines. Inasmuch as Paulownia elongata endures urban conditions well, it can be recommended for cultivation in parks, tree alleys, and wind-protection zones along urban and regional traffic lines.

  4. Hu-Lu-Ba-Wan Attenuates Diabetic Nephropathy in Type 2 Diabetic Rats through PKC-α/NADPH Oxidase Signaling Pathway

    Directory of Open Access Journals (Sweden)

    Lishan Zhou

    2013-01-01

    Full Text Available Hu-Lu-Ba-Wan (HLBW is a Chinese herbal prescription used to treat kidney deficiency. The aim of this study was to explore the effect and mechanism of HLBW on diabetic nephropathy (DN in type 2 diabetic rats. The rat model of DN was established by being fed a high-fat diet and intravenous injection of streptozotocin. Then, HLBW decoction was administered for 16 weeks. Blood glucose level, lipid profile, renal function, 24-hour total urinary protein, and albumin content were examined. Renal morphology and superoxide anion levels were evaluated. The activity of nicotinamide-adenine dinucleotide phosphate (NADPH and protein kinase C-alpha (PKC-α related genes expression in renal tissue were also determined. Our data demonstrated that HLBW significantly improved hyperglycemia, hyperlipidemia, and proteinuria in diabetic rats compared with those of control group. HLBW also alleviated glomerular expansion and fibrosis, extracellular matrix accumulation and effacement of the foot processes. Additionally, HLBW reduced superoxide anion level, NADPH oxidase activity, the protein and mRNA expressions of p47phox, and the protein expression of phosphorylated PKC-α in renal tissue. These results suggest that HLBW is effective in the treatment of DN in rats. The underlying mechanism may be related to the attenuation of renal oxidative stress via PKC-α/NADPH oxidase signaling pathway.

  5. Research on the Wetland Ecotourism Products Development in Chang hu Lake%长湖湿地生态旅游产品开发研究

    Institute of Scientific and Technical Information of China (English)

    袁龙义; 龙利华

    2011-01-01

    介绍了长湖湿地的概况和研究现状,结合湿地的研究理论和长湖自身的特点,提出了长湖湿地生态旅游开发的原则,并列举出长湖湿地旅游产品开发的具体方案,分为特色专项、观光、人文历史和休闲度假生态旅游产品4个系列,其中观鸟、垂钓、"农家乐"等都是长湖非常有特色的旅游开发项目.%The general situation of ChangHu wetland and the current research are introduced in this paper. Combining with the study theory of wetland and the characteristics of Changhu wetland, principles of Changhu wetland ecotourism development are proposed and also the detail programs of tourism product development are listed as: special characteristics, sightseeing, cultural history, leisure vacation and so on. Among them, the bird-watching, fishing and fanning happiness are the most characteristic tourism development project.

  6. Effect of nutrition on plasma lipid profile and mRNA levels of ovarian genes involved in steroid hormone synthesis in Hu sheep during luteal phase.

    Science.gov (United States)

    Ying, S J; Xiao, S H; Wang, C L; Zhong, B S; Zhang, G M; Wang, Z Y; He, D Y; Ding, X L; Xing, H J; Wang, F

    2013-11-01

    Ovarian steroid hormones regulate follicular growth and atresia. This study aims to determine whether key ovarian sterol-regulatory genes are differentially expressed in Hu sheep under different short-term nutritional regimens. Estrus was synchronized using intravaginal progestagen sponges. The ewes were assigned randomly to 3 groups. On d 6 to 12 of their estrous cycle, the control (CON) group received a maintenance diet (1.0×M), the supplemented (SUP) group received 1.5×M, and the restricted (R) group received 0.5×M. On d 7 to 12, blood samples were taken. The sheep were slaughtered at the end of the treatment, and their organs and ovaries were collected. The plasma concentrations of urea (P2.5 mm. Follicle size affected the mRNA expression of very low density lipoprotein receptor (VLDLR), estrogen receptor 2 (ESR2), FSH receptor (FSHR), CYP17A1, and CYP19A1 (P<0.05). In conclusion, we suggest that a potential mechanism by which short-term negative energy balance inhibits follicular growth may involve responses to disrupted reproductive hormone concentrations and influenced the intrafollicular expression of CYP17A1, CYP19A1, and ESR1. This result may be due to increased plasma urea and lipid concentrations.

  7. 培养条件对嗜热芽孢杆菌HU1合成几丁质降解酶及脱乙酰基酶的影响%Effects of Culture Conditions on the Synthesis of Chitinase and Chitin Deacetylase from Thermophilic Bacillus sp. HU1

    Institute of Scientific and Technical Information of China (English)

    戴德慧; 胡伟莲; 李巍

    2011-01-01

    [ Objective ] The aims were to investigate the effects of culture conditions on the enzyme production by Thermophilic Bacillus sp.Hul and provide basis for fermentation of enzyme and preparation of chitosan oligosaccharide. [ Method] The shake flask culture was employed to research the culture conditions of Thermophilic Bacillus sp. for producing Chitinase and Chitin Deacetylase HU1. [ Result] The optimum carbon source was powder chitinase with the optimum addition of 3.5%; while yeast power was the optimum nitrogen source with the optimal addition of 1.0%. It was beneficial to synthesize chitinase and chitin deacetylase when the original pH was 6.0. In addition, the synthesis of chitinase and chitin deacetylaso was inhibited by Cu2+ , and activated by Ca2+. However, Mg2+ and Zn2+ did not show significant inhibitory effects on the synthesis of the two enzymes. Moreover, the production of chitinaso and chitin deacetylase reached peak after culture for 72 h.The hydrolysate components of the crude enzyme solution were analyzed by using powder chitin as substrate, and the result suggested that chitosan oligosaccharide with the molecular weight of less than hexaose was the main component. [ Conclusion ] The preparation process of chitosan oligosaccharide of small molecules by using crude enzyme solution was simple, which could provided a basis for the further developments of health care production, biological pesticide, animal feed additive and food additive.%[目的]研究培养条件对嗜热芽孢杆菌HU1产酶的影响,为工业化发酵生产酶及壳寡糖的制备提供依据.[方法]采用摇瓶培养方式,对嗜热芽孢杆菌HU1产几丁质降解酶及脱乙酰基酶的培养条件进行研究.[结果]最佳诱导碳源为粉末几丁质,其最适添加量为3.5%;最佳氮源为酵母粉,最适添加量为1.0%;初始pH值为6.0时,有利于产酶;Cu2+对酶的合成表现出明显的抑制作用;而Ca2+则能有效增加产酶能力.Mg2+及Zn2+对嗜热芽孢杆菌HU

  8. Reproduction de l’huître perlière : évaluation des besoins en acides gras et stérols au cours de l’ovogenèse chez l’huître perlière Pinctada margaritifera

    OpenAIRE

    Le Moullac, Gilles; Vairuha-lechat, Isabelle; Bianchini, Jean Pierre; Le Coz, Jean-rene; Tiapari, Jerome; Laure, Frédéric; Mitermite, Yves

    2004-01-01

    Cette étude financée par le Ministère de l’Outre-Mer est une collaboration de l’Ifremer avec l’Université de Polynésie Française et l’Institut Louis Malardé. Cette étude a permis l’acquisition de connaissances nouvelles sur la nutrition de l’huître perlière Pinctada margaritifera dans le milieu naturel, et ainsi, de mieux envisager les besoins nutritionnels pendant la reproduction. Les besoins en lipides, acides gras et stérols, ont été spécifiquement étudiés. La composition en acides gra...

  9. Observation on the curative effect of Feng' s rotatory reduction manipulation at fixed points of spine in the treatment of acute lumbar sprain%冯氏脊柱定点旋转复位手法治疗急性腰扭伤的疗效观察

    Institute of Scientific and Technical Information of China (English)

    杜诚恳; 董倔强; 王海磊; 翁文水

    2012-01-01

    目的:观察冯氏脊柱定点旋转复位手法治疗急性腰扭伤的临床疗效.方法:将纳入研究的90例急性腰扭伤患者随机分成2组,每组45例.治疗组采用冯氏脊柱定点旋转复位手法治疗,对照组采用传统斜扳手法治疗 手法治疗每天1次,共治疗3次.分别于治疗前和治疗结束后采用自拟腰痛疾患疗效评定标准对2组患者的疗效进行评定.结果:治疗前2组患者腰痛评分比较,差异无统计学意义(t=0.028,P=0.978);治疗后2组患者腰痛评分均增加(t=18.183,P=0.000;t=13.726,P=0.000),治疗组增加更明显(t=2.039,P=0.044).结论:冯氏脊柱定点旋转复位手法治疗急性腰扭伤疗效显著,值得临床推广应用.%Objective:To observe the clinical curative effect of Feng' s rotatory reduction manipulation at fked points of spine in the treatment of acute lumbar sprain( ALS). Methods:Ninety ALS patients enrolled in the study were randomly divider! into 2 groups,45 cases in each group. Patients in the treatment group were administrated with Feng's rotatory reduction manipulation at fixed points of spine, while the others in the control group were administrated with traditional oblique-pulling manipulation,once a day for consecutive 3 days. The cura-tive effects were evaluated according to the self-designed standard for curative effect evaluation of low back pain before and after the treat-ment respectively. Results:There was no statistical difference in the low back pain scores between the 2 groups before the treatment (t = 0. 028, P = 0.978). The low back pain scores of the 2 groups were all increased after the ireatmen(t = 18. 183 ,P = 0. 000;/ = 13.726.t = 0. 000) ,and scores increased more significantly in the treatment group(t =2. 039 ,P=0. 044 ). Conclusion;The method of Feng' s rotatory reduction manipulation at fixed points of spine performs well in ihe. treatment of acute lumbar sprain.it is worth popularizing in the clinic.

  10. On the Theoretical Contribution to the Massification of Marxist Philosophy of Shen Hu%胡绳对马克思主义哲学大众化的理论贡献研究

    Institute of Scientific and Technical Information of China (English)

    张华

    2014-01-01

    Shen Hu wrote a lot of works on the massification of Marxist Philosophy from Anti-Japanese War to the founding of the People’ s Republic of China. Exploring the thought of the massification of Marxist Philosophy by collecting and collating those works systematically, it will give impetus to the research on the academic thought of Shen Hu.%胡绳的马克思主义哲学大众化文章、著作颇丰,散见于抗战前、抗战中、解放战争及新中国成立后不同历史时期。系统搜集和整理这些作品,深入挖掘胡绳关于马克思主义哲学大众化思想,有助于推进胡绳学术思想的全面深入研究。

  11. Comment on "Observation of mutual diffusion of macromolecules in PS/PMMA binary films by confocal Raman microscopy" by C. Hu, X. Chen, J. Chen, W. Zhang and M. Q. Zhang, Soft Matter, 2012, 8, 4780.

    Science.gov (United States)

    Tomba, J Pablo

    2016-05-18

    A paper by Hu et al. (Soft Matter, 2012, 8, 4780) reports on the use of confocal Raman microscopy to resolve mutual diffusion between polystyrene (PS) and poly(methyl methacrylate) (PMMA). In-depth optical sectioning is employed to measure the diffusive broadening of the originally planar PS-PMMA interface, from which tracer and mutual diffusion coefficients and values for the PS-PMMA thermodynamic interaction parameter are extracted. Here, a reinterpretation of Hu's data that leads to a completely different scenario is presented, as apparent diffusive broadening can be mostly attributed to optical distortions inherent to the probe methodology. It also explains the lack of consistency of kinetic and thermodynamic parameters obtained by the authors from their diffusion analysis in comparison with earlier published data on this system. Overall, it highlights the importance of carrying out appropriate data analysis when confocal Raman microscopy is applied in dry depth-profiling investigations.

  12. Study on Comparison of Growth and Development in Crossbreeding Offspring between Suffolk and Hu Sheep in Xinjiang Scale Sheep Farm%新疆规模羊场萨福克与湖羊杂交应用试验

    Institute of Scientific and Technical Information of China (English)

    桂东城; 杨华; 宋文富; 张华; 杨保华; 谭现宁

    2015-01-01

    为建立适应新疆规模化羊场的杂交生产体系,提高种羊利用率及当地绵羊的生产性能,以萨福克羊为父本、湖羊为母本,在规模羊场开展杂交应用试验。以出生日龄相近的萨湖F1为试验组,湖羊羔羊为对照组,测定初生重、2月龄重、4月龄重、6月龄体重、6月龄体尺等生长指标及宰前活重、胴体重、净肉重、屠宰率和净肉率等屠宰性能指标。结果表明,萨湖F1羔羊的2月龄重、4月龄重、6月龄体重及日增重显著高于湖羊F1羔羊(<0.05),6月龄萨湖F1羔羊平均宰前活重、胴体重、净肉重、屠宰率及净肉率显著高于湖羊F1羔羊(<0.05)。利用萨福克杂交改良规模羊场湖羊,可以显著提高羔羊的生长发育,杂种优势显著。%In order to establish the breeding and hybridization production system to adapt to the Xinjiang scale sheep farm, improve the production performance of local sheep breed and utilization rate of ram. The hybridization experiment were done by Suffolk sheep crossing Hu sheep in farm. Suffolk×Hu F1 lambs were as the trial group, Hu F1 lambs were as the control group, which had the same age. The growth indicators, such as birth weight, 2―months old body weight, 4―months old body weight, 6―months old body weight and body size, were measured. The slaughter performance indicators, such as body weight, carcass weight, meat weight, slaughter percentage rate and meat percentage rate, were also measured. The results show, under the same raising conditions, 2―months old body weight, 4―months old body weight, 6―months old body weight and average daily gain of Suffolk×Hu F1 lambs were significantly higher than Hu F1 lambs ( <0.05). The average body weight, carcass weight, meat weight, slaughter percentage rate and meat percentage rate of Suffolk×Hu F1 lambs were significantly higher than Hu F1 lambs in 6―months old ( <0.05). It was showed that hybridization can significantly improve meat

  13. 124I-HuCC49deltaCH2 for TAG-72 antigen-directed positron emission tomography (PET imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results

    Directory of Open Access Journals (Sweden)

    Mojzisik Cathy M

    2010-08-01

    Full Text Available Abstract Background 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET is widely used in diagnostic cancer imaging. However, the use of 18F-FDG in PET-based imaging is limited by its specificity and sensitivity. In contrast, anti-TAG (tumor associated glycoprotein-72 monoclonal antibodies are highly specific for binding to a variety of adenocarcinomas, including colorectal cancer. The aim of this preliminary study was to evaluate a complimentary determining region (CDR-grafted humanized CH2-domain-deleted anti-TAG-72 monoclonal antibody (HuCC49deltaCH2, radiolabeled with iodine-124 (124I, as an antigen-directed and cancer-specific targeting agent for PET-based imaging. Methods HuCC49deltaCH2 was radiolabeled with 124I. Subcutaneous tumor implants of LS174T colon adenocarcinoma cells, which express TAG-72 antigen, were grown on athymic Nu/Nu nude mice as the xenograft model. Intravascular (i.v. and intraperitoneal (i.p. administration of 124I-HuCC49deltaCH2 was then evaluated in this xenograft mouse model at various time points from approximately 1 hour to 24 hours after injection using microPET imaging. This was compared to i.v. injection of 18F-FDG in the same xenograft mouse model using microPET imaging at 50 minutes after injection. Results At approximately 1 hour after i.v. injection, 124I-HuCC49deltaCH2 was distributed within the systemic circulation, while at approximately 1 hour after i.p. injection, 124I-HuCC49deltaCH2 was distributed within the peritoneal cavity. At time points from 18 hours to 24 hours after i.v. and i.p. injection, 124I-HuCC49deltaCH2 demonstrated a significantly increased level of specific localization to LS174T tumor implants (p = 0.001 when compared to the 1 hour images. In contrast, approximately 50 minutes after i.v. injection, 18F-FDG failed to demonstrate any increased level of specific localization to a LS174T tumor implant, but showed the propensity toward more nonspecific uptake within the

  14. 舞台空间艺术的表现世界--胡佐舞台设计作品主创谈%Performance World of Stage Space Art- Key Creatives Talk about Hu Zuo's Stage Design Production

    Institute of Scientific and Technical Information of China (English)

    蓝玲; 胡佐

    2015-01-01

    点评胡佐的桂剧《七步吟》,越剧《班昭》、《大道行吟》,黄梅戏《寂寞汉卿》四部作品的舞台美术设计。%The paper commented on Hu Zuo's four stage design productions of Gui operaSeven Steps, Yue operaBanZhao, Avenue Line, and Huangmei OperaLonely Hanqing.

  15. Study of Hu Xishu's Medical Thoughts on Impotence, Emission And Prospermia%从胡希恕医学思想浅析阳痿、遗精、早泄

    Institute of Scientific and Technical Information of China (English)

    任伟明; 谭映辉; 刘文琛; 张熙; 贺菊乔; 谢明霞

    2015-01-01

    阳痿、遗精、早泄是临床上男科常见疾病,胡希恕善用经方治疗.笔者根据伤寒原文结合先生医案及自身临床经验分析,先生辨遗精、早泄多由下焦虚寒、虚阳上亢所致,故临症常以桂枝加龙骨牡蛎汤合熟附子、白薇治疗;而阳痿则主因阳气虚于下,气虚郁结血水停滞,阳气不达所致,故常以四逆散合当归芍药散为基础治之,疗效显著.%Hu Xishu is experienced with classical prescription medication for treating Impotence, emission and prospermia that are common in andrology. And the writer analyzed Hu's clinical thoughts based on original text ofTreatise on Exogenous Febrile Diseases, Hu's case records and the writer's own clinical experiences. For emission and prospermia, Professor Hu differentiated the syndromes as lower-jiao deficiency cold and hyperactivity of deficient yang, and medicated Guizhi Jia Longgu Muli Decoction added with prepared aconite root and cynanchum atratum for treatment; while impotence was differentiated as lower-jiao deficiency of yang, deficiency and stagnation of qi, retention of blood and water leading to yang-qi no reaching and medicated Sini Powder combined with Danggui Shaoyao Powder, with significant effects.

  16. Geología y paleontología de los yacimientos plio-pleistocenos de Huéscar (Depresión de Guadix-Baza. Granada

    Directory of Open Access Journals (Sweden)

    Mazo, A. V.

    1985-12-01

    Full Text Available In this work, two new vertebrare fossil faunas from the Guadix-Baza Depression (Granada, Spain are studied. Huéscar 3 is characterized by Castillomys crusafonti, Mimomys stehlini, Blancomys neglectus, Thischizolagus aff. maritsae and Anancus arvernensis; This site, which belong to the upper Ruscinian (MN 15 of Mein can be correlated with the localities of Arquillo 3, Villalba Alta, Sête and Seines. Huéscar 1, with Mimomys savini, Microtus brecciensis, cf. Microtus (Pitymys gregaloides, Hippopotamus antiquus and Mammuthus meridionalis, can be placed in the lower part of the middle Pleistocene and can be correlated with the Le Vallonet fossil site.

    En el presente trabajo se estudian dos nuevos yacimientos de vertebrados fósiles de la Depresión do Guadix-Baza (Granada. Huéscar 3, caracterizado por Castillomys crusafonti, Mimomys stehlini, Blancomys neglectus, Trischizolagus aff. maritsae y Anancus arvernensis, se sitúa en el Rusciniense superior (MN 15 de Mein, siendo correlacionable con las locali dades de Arquillo 3, Villalba Alta, Sête y Seines. Huéscar 1, con Mimomys savini, Microtus brecciensis, cf. Microtus (Pitymys gregaloides, Hippopotamus antiquus y Mammuthus meridionalis, se atribuye a la parte inferior del Pleistoceno medio, correlacionable con el yacimiento de Le Vallonet

  17. Selective Regulation of Human Immunodeficiency Virus-Infected CD4+ Lymphocytes by a Synthetic Immunomodulator Leads to Potent Virus Suppression In Vitro and in hu-PBL-SCID Mice

    OpenAIRE

    Bahr, George M.; Darcissac, Edith C. A.; Castéran, Nathalie; Amiel, Corinne; Cocude, Cécile; Truong, Marie-José; Dewulf, Joëlle; Capron, André; Mouton, Yves

    2001-01-01

    We have previously observed that the synthetic immunomodulator Murabutide inhibits human immunodeficiency virus type 1 (HIV-1) replication at multiple levels in macrophages and dendritic cells. The present study was designed to profile the activity of Murabutide on CD8-depleted phytohemagglutinin-activated lymphocytes from HIV-1-infected subjects and on the outcome of HIV-1 infection in severe combined immunodeficiency mice reconstituted with human peripheral blood leukocytes (hu-PBL-SCID mic...

  18. 拟缺香茶菜化学成分的研究%Studies on the chemical constituents of Isodon excisoides(Sun ex C.H.Hu) C.Y.Wu et H.W.Li

    Institute of Scientific and Technical Information of China (English)

    丁兰; 王瀚; 刘国安; 杨东娟

    2005-01-01

    从甘肃产拟缺香茶菜(Isodon excisoides(Sun ex C.H.Hu)C.Y.Wu et H.W.Li)的叶中分离得到6个化合物,利用现代波谱学方法鉴定其结构为:β-谷甾醇,熊果酸,线蓟素,胡麻素,胡萝卜甙,槲皮甙.

  19. A Comparative Study on the Values of West Hu'nan's Na-tive Mythology and Greek Mythology%湘西本土神话与古希腊神话价值观比较研究

    Institute of Scientific and Technical Information of China (English)

    陈娜

    2016-01-01

    一个民族对自然界和历史的认识通常会反映到其流传的神话中,作为古老东方神话重要组成部分的湘西本土神话因其独特地域性,与西方古希腊神话所表现出来的价值观大相径庭。本文拟以湘西本土神话中的创世神话与古希腊神话中奥林匹斯神话价值观对比研究,初步探讨东西方先民对人文主义的认识及其蕴含的文化内涵。%A nation's knowledge of the natural world and his-tory is generally reflected in the widespread myths among the nation. As an important component of the ancient Oriental my-thology, west Hu'nan's native mythology, with its own unique regional characteristics, carries its values that are quite different from that of Occidental Greek mythology. Through a compara-tive study on the creation myth in west Hu'nan's mythology and the myths of Olympus in Greek mythology, this paper intends to preliminarily explore Occidental ancestors' knowledge of hu-manism and the cultural connotation embodied in it.

  20. Mitilanotherium inexpectatum (Giraffidae, Mammalia from Huélago (Lower Pleistocene; Guadix-Baza basin, Granada, Spain - observations on a peculiar biogegraphic pattern

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    Morales, J.

    2011-12-01

    Full Text Available Giraffid fossils from the lowermost Pleistocene (MN17 locality Huélago (Guadix-Baza Basin, Granada, Spain are described and assigned to Mitilanotherium inexpectatum Samson & Radulesco (1966. The remains are compared to giraffid material from the Pliocene and younger. The known geographic distribution of this giraffid is disjunct; it is found in Spain and in an area stretching from Rumania and Greece to Tadzhikistan, but not in central Europe. The oldest record is from the Upper Pliocene (MN16 of Turkey and the youngest is from the Lower Pleistocene of Greece (with an estimated age of about 1.2 Ma. Shortly after 2.6 Ma it may have dispersed to Spain, where it may have lived as much as half a million years. The dispersal did not leave a fossil record in the area between SE Europe and Spain. The same occured with dispersals of other mammals in the Early, Middle and Late Miocene, Pliocene and Pleistocene. These species that show this pattern are interpreted to be adapted to open or arid environments. Their dispersals across Europe to Spain may have occurred during short periods of atypical environmental conditions and thus did not leave an easily detectable fossil record.Los fósiles de jirafas del Pleistoceno basal (MN 17 de la localidad de Huélago (Cuenca de Guadix- Baza, Granada, Spain son descritos y asignados a Mitilanotherium inexpectatum Samson & Radulesco (1966. Los restos fósiles son comparados con jiráfidos del Plioceno, y formas más recientes. La distribución geográfica conocida muestra que es disyunta; encontrándose en España y en un área que se extiende de Rumania y Grecia a Tadzhikistan, pero no en Europa central. El registro más antiguo procede del Plioceno Superior (MN 16 de Turquía y el más reciente del Pleistoceno inferior de Grecia (con una edad estimada de ca. 1, 2 Ma. Poco después de los 2,6 Ma la especie pudo haberse dispersado a España, donde como mucho pudo haber durado medio millón de años. Esta dispersion