Frequency of helicobacter pylori (hp) infection in children with recurrent abdominal pain (rap)

International Nuclear Information System (INIS)

Mahmud, S.; Ali, S.

2015-01-01

To study the frequency of Helicobacter Pylori (HP) infection among children with recurrent abdominal pain (RAP) Study Design: Cross-sectional comparative study. Place and Duration of Study: Military Hospital (MH), Rawalpindi from December 2011 to February 2012. Patients and Methods: One hundred children of either gender aged 2 to 12 years presenting with RAP were tested for HP at Paediatric OPD MH, Rawalpindi who consented to participate in the study. Those children who tested positive for Helicobacter Pylori Stool Antigen Test (HPSAT) were labeled as those having Hp infection. The stool assay was performed using the HpSAT kit and the socio-demographic and clinical profiles of children were associated. Results: Out of 100 children included in the study HpSAT was positive in 38% children. Frequency of Hp infection was significantly associated with source of drinking water (p = 0.014), socioeconomic status (p = 0.001) and positive family history of yspepsia (p= 0.023). While age and gender have no significant association with HP infection. Conclusion: Hp infection is very common in children presenting with RAP in our Paediatric OPD. Children with family history of dyspepsia, from low socioeconomic class and those drinking filtered water are at greater risk for HP infection. It is recommended that children from other populations in our country should also be tested in their medical health facilities in order to have a wider analysis of this problem in our setup. (author)

Operation of the HP2250 with the HP9000 series 200 using PASCAL 3.0

Science.gov (United States)

Perry, John; Stroud, C. W.

1986-01-01

A computer program has been written to provide an interface between the HP Series 200 desktop computers, operating under HP Standard Pascal 3.0, and the HP2250 Data Acquisition and Control System. Pascal 3.0 for the HP9000 desktop computer gives a number of procedures for handling bus communication at various levels. It is necessary, however, to reach the lowest possible level in Pascal to handle the bus protocols required by the HP2250. This makes programming extremely complex since these protocols are not documented. The program described solves those problems and allows the user to immediately program, simply and efficiently, any measurement and control language (MCL/50) application with a few procedure calls. The complete set of procedures is available on a 5 1/4 inch diskette from Cosmic. Included in this group of procedures is an Exerciser which allows the user to exercise his HP2250 interactively. The exerciser operates in a fashion similar to the Series 200 operating system programs, but is adapted to the requirements of the HP2250. The programs on the diskette and the user's manual assume the user is acquainted with both the MCL/50 programming language and HP Standard Pascal 3.0 for the HP series 200 desktop computers.

Haptoglobin genotyping of Vietnamese: global distribution of HP del, complete deletion allele of the HP gene.

Science.gov (United States)

Soejima, Mikiko; Agusa, Tetsuro; Iwata, Hisato; Fujihara, Junko; Kunito, Takashi; Takeshita, Haruo; Lan, Vi Thi Mai; Minh, Tu Binh; Takahashi, Shin; Trang, Pham Thi Kim; Viet, Pham Hung; Tanabe, Shinsuke; Koda, Yoshiro

2015-01-01

The haptoglobin (HP) gene deletion allele (HP(del)) is responsible for anhaptoglobinemia and a genetic risk factor for anaphylaxis reaction after transfusion due to production of the anti-HP antibody. The distribution of this allele has been explored by several groups including ours. Here, we studied the frequency of HP(del) in addition to the distribution of common HP genotypes in 293 Vietnamese. The HP(del) was encountered with the frequency of 0.020. The present result suggested that this deletion allele is restricted to East and Southeast Asians. Thus, this allele seems to be a potential ancestry informative marker for these populations. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

HP 9816

CERN Multimedia

1982-01-01

The 9816 was introduced in late 1982. This was the low-cost model in the 200 Series range. It only had two expansion slots and featured a monitor integrated with the system unit and modular keyboard and mass storage (usually a 9121 dual 3.5 inch floppy drive). The monitor was nine inches diagonally with a 400 by 300 dot resolution. The HP 9816 was also designated as the HP 9000 216. It did not include any disk drives but it had a built-in 9 inch monochrome monitor, built-in HP-IB and RS-232 ports and 2 expansion slots. The standard keyboard for the 9816 is a itty-bitty number. The 9816 A came with 128K bytes of memory. The 9816 S included all of the above plus disk based BASIC and a card containing an additional 256K of memory bringing the total memory to 512K but only leaving only one expansion slot open.

On Adjusting the H-P Filter for the Frequency of Observations

NARCIS (Netherlands)

Uhlig, H.F.H.V.S.; Ravn, M.

1997-01-01

This paper studies how the HP-Filter should be adjusted, when changing the frequency of observations. The usual choices in the literature are to adjust the smoothing parameter by multiplying it with either the square of the observation frequency ratios or simply with the observation frequency. In

Weekly cycle of magnetic characteristics of PM2.5 and PM2.5-10 in Beijing, China

Science.gov (United States)

SHI, M.; Wu, H.; Zhang, S.; Li, H.; Yang, T.

2013-12-01

In urban areas,fine particle matter with aerodynamic diameter between 2.5 um and 10 um (PM2.5-10), and 2.5 um (PM2.5), as an important source of urban particulate matter (PM) pollutants, have significant negative effects on health, atmospheric visibility and climate. PM has increasingly become a significant index of indicating the atmospheric pollution of city. In recent years, Beijing, China has been listed as one of the most serious air pollution city in the world. In order to investigate the sources of air pollutants, a total of 283 pairs of PM2.5 and PM2.5-10 samples were collected daily from July, 2010 to June, 2011 in Beijing. Mineral magnetic properties and Scanning electron microscope (SEM) observations and energy dispersive X-ray spectroscopy (EDS) analyses of PM2.5 and PM2.5-10 were measured to verify the magnetic materials. Magnetic measures for PM indicated that the major magnetic phase was coarse-grained magnetite-like material. The χlf, χarm, SIRM and χarm/SIRM series of the PM2.5 and PM2.5-10 show seasonal dependences: high values in winter and low values in summer. In additional the parameters analyzed by Time-series methods show a strong cycle about 7 days above 95% confidence level. Weekly cycle of magnetic characteristics of PM2.5 and PM2.5-10 show different pattern: the concentration of magnetic particles in PM2.5-10 show high values in mid-week, and particle sizes is steady, while the concentration of magnetic particles in PM2.5 show reverse a weekly cycle pattern, and particle sizes is smaller in the mid-week.Microscopy analyses reveal basically three morphologies of magnetic grains: aggregate, spherules and angular particles. The ultrafine carbonaceous particles which tend to form complex clusters and chain-like structures, most likely come from coal burning and motor vehicle exhaust. Spherical particles in PM2.5 are dominantly composed of Fe, O and C, grain-diameters of particles range from 0.3 to 2 um. Angular particles of Fe

HP1γ function is required for male germ cell survival and spermatogenesis

Directory of Open Access Journals (Sweden)

Brown Jeremy P

2010-04-01

Full Text Available Abstract Background HP1 proteins are conserved components of eukaryotic constitutive heterochromatin. In mammals, there are three genes that encode HP1-like proteins, termed HP1α, HP1β and HP1γ, which have a high degree of homology This paper describes for the first time, to our knowledge, the physiological function of HP1γ using a gene-targeted mouse. Results While targeting the Cbx3 gene (encoding the HP1γ protein with a conditional targeting vector, we generated a hypomorphic allele (Cbx3hypo, which resulted in much reduced (barely detectable levels of HP1γ protein. Homozygotes for the hypomorphic allele (Cbx3hypo/hypo are rare, with only 1% of Cbx3hypo/hypo animals reaching adulthood. Adult males exhibit a severe hypogonadism that is associated with a loss of germ cells, with some seminiferous tubules retaining only the supporting Sertoli cells (Sertoli cell-only phenotype. The percentage of seminiferous tubules that are positive for L1 ORF1 protein (ORF1p in Cbx3hypo/hypo testes is greater than that for wild-type testes, indicating that L1 retrotransposon silencing is reversed, leading to ectopic expression of ORF1p in Cbx3hypo/hypo germ cells. Conclusions The Cbx3 gene product (the HP1γ protein has a non-redundant function during spermatogenesis that cannot be compensated for by the other two HP1 isotypes. The Cbx3hypo/hypo spermatogenesis defect is similar to that found in Miwi2 and Dnmt3L mutants. The Cbx3 gene-targeted mice generated in this study provide an appropriate model for the study of HP1γ in transposon silencing and parental imprinting.

INCREASED AIRWAYS INFLAMMATION AND MODIFIED BAL CELL SURFACE PHENOTYPES IN ASTHMATICS EXPOSED TO COARSE SIZE (PM2.5-10) CONCENTRATED AMBIENT PARTICLES (CAPS)

Science.gov (United States)

Although associations between inhalation of PM10 and disease morbidity and mortality appear stronger for fine (PM2.5) vs coarse (PM2.5-10) or ultrafine/UF (PM<0.1) PM. In vitro studies suggest that PM2.5-10 are more potent in inducing pro-inflammatory cytokine responses from alve...

Study of the quasi-free scattering at the reaction 2H(p,2p)n at Esub(p)0 = 14.1 MeV

International Nuclear Information System (INIS)

Helten, H.J.

1980-01-01

The breakup reaction 2 H(p,2p)n was studied at Ep = 14.1 MeV in complete coincidence experiments on quasifree pp scattering in a systematic range of cinematic situations of the pp-subsystem for c.m. production angles between 90 0 and 140 0 and different violation of the quasifree condition as well on interferences with final-state interaction processes. The absolute differential breakup cross section was compared with approximate solutions of the Faddeev equations with separable s-wave potentials without explicite Coulomb interaction according to Ebenhoeh. The agreement is generally good referring to the form of the spectra, but the theoretical amplitude is in the mean 20% to high. The permanent independence of the quasifree breakup from the scattering parameter asub(pp) doesn't suggest to use this process for the determination of nn-scattering lengths from the mirror reaction 2 H(n,2n)p. (orig.)

Addendum report of the JHPS expert committee on radiation protection of the lens of the eye (2). When and how should the dosimetry of beta H_p(3) be made?

International Nuclear Information System (INIS)

Akahane, Keiichi; Tatsuzaki, Hideo; Iimoto, Takeshi; Ichiji, Takeshi; Hamada, Nobuyuki; Iwai, Satoshi; Ohguchi, Hiroyuki; Ohno, Kazuko; Kawaura, Chiyo; Kurosawa, Tadahiro; Tsujimura, Norio; Hayashida, Toshiyuki; Hotta, Yutaka; Yamasaki, Tadashi; Yokoyama, Sumi

2015-01-01

In a mixed field of photon and beta radiations, the same dose assigned to skin is normally assigned to the dose to the lens of the eye as a conservative estimate of H_p(3). In exceptional cases where a very high beta dose might be imparted of the same order with the dose limit, however, the conservatively biased dose must be too limiting, and thereby an accurate estimate of beta H_p(3) is desirable. This addendum report of the Japan Health Physics Society Expert Committee on Radiation Protection of the Lens of the Eye presents a practical proposal of when and how the dosimetry of beta H_p(3) should be made. (author)

[Relationships between the enrichment of ETBF, Fn, Hp in intestinal and colorectal cancer].

Science.gov (United States)

Zhang, J; Lu, X L; Zhao, G; Shi, H T; Geng, Y; Zhong, W T; Dong, L

2018-02-23

Objective: To explore relationships between the enrichment of ETBF, Fn, Hp in feces, tissues and colorectal cancer. Methods: Feces, lesion tissue and adjacent tissue from 24 patients with colorectal cancer and 31 patients with adenomas were collected, and we collected Feces and tissue of 20 healthy control persons. Then the copy numbers of enterotoxigenic B. fragilis (ETBF), Fusobacterium nucleatum (Fn) and Helicobacter pylori (Hp) were determined by quantitative real-time PCR. Immunohistochemical method was used to examine the expression intensity of EGFR and p53, and the relationships between different expression intensity of EGFR, p53 and the numbers of three bacterias. Results: In the feces, copy numbers of ETBF and Fn were as follous: colorectal cancer group>adenomas group>healthy control group ( P healthy control group ( P healthy control group ( P adenomas group>healthy control group ( P healthy control group ( P healthy control group ( P bacteria in the lesion tissue and the adjacent tissue had no significant difference. This happened both in colorectal cancer group and adenomas group. The different expression intensity of EGFR, p53 and the number of three bacteria showed no obviously statistical correlation( P >0.05). Conclusion: Adenomatous polyp and colorectal cancer patients show high enrichment of ETBF, Fn and Hp in both feces and tissues. ETBF, Fn and Hp probably contribute to the development of adenomatous polyp and colorectal cancer. Trial registration Chinese Clinical Trial Registry, ChiCTR-BOC-17012509.

75 FR 62424 - EDS, an HP Company (Re-Branded as HP-Enterprise Services) Including On-Site Workers From: Abel...

Science.gov (United States)

2010-10-08

...-Branded as HP--Enterprise Services) Including On-Site Workers From: Abel Personnel Inc., Advantage Tech... February 4, 2010, applicable to workers of EDS, an HP Company (Re- branded as HP--Enterprise Services...-branded as HP-- Enterprise Services). These employees provided various activities related to the supply of...

Spectral/ hp element methods: Recent developments, applications, and perspectives

Science.gov (United States)

Xu, Hui; Cantwell, Chris D.; Monteserin, Carlos; Eskilsson, Claes; Engsig-Karup, Allan P.; Sherwin, Spencer J.

2018-02-01

The spectral/ hp element method combines the geometric flexibility of the classical h-type finite element technique with the desirable numerical properties of spectral methods, employing high-degree piecewise polynomial basis functions on coarse finite element-type meshes. The spatial approximation is based upon orthogonal polynomials, such as Legendre or Chebychev polynomials, modified to accommodate a C 0 - continuous expansion. Computationally and theoretically, by increasing the polynomial order p, high-precision solutions and fast convergence can be obtained and, in particular, under certain regularity assumptions an exponential reduction in approximation error between numerical and exact solutions can be achieved. This method has now been applied in many simulation studies of both fundamental and practical engineering flows. This paper briefly describes the formulation of the spectral/ hp element method and provides an overview of its application to computational fluid dynamics. In particular, it focuses on the use of the spectral/ hp element method in transitional flows and ocean engineering. Finally, some of the major challenges to be overcome in order to use the spectral/ hp element method in more complex science and engineering applications are discussed.

Ethnicity/culture modulates the relationships of the haptoglobin (Hp) 1-1 phenotype with cognitive function in older individuals with type 2 diabetes.

Science.gov (United States)

Guerrero-Berroa, Elizabeth; Ravona-Springer, Ramit; Heymann, Anthony; Schmeidler, James; Hoffman, Hadas; Preiss, Rachel; Koifmann, Keren; Greenbaum, Lior; Levy, Andrew; Silverman, Jeremy M; Leroith, Derek; Sano, Mary; Schnaider-Beeri, Michal

2016-05-01

The haptoglobin (Hp) genotype has been associated with cognitive function in type 2 diabetes. Because ethnicity/culture has been associated with both cognitive function and Hp genotype frequencies, we examined whether it modulates the association of Hp with cognitive function. This cross-sectional study evaluated 787 cognitively normal older individuals (>65 years of age) with type 2 diabetes participating in the Israel Diabetes and Cognitive Decline study. Interactions in two-way analyses of covariance compared Group (Non-Ashkenazi versus Ashkenazi Jews) on the associations of Hp phenotype (Hp 1-1 versus non- Hp 1-1) with five cognitive outcome measures. The primary control variables were age, gender, and education. Compared with Ashkenazi Jews, non-Ashkenazi Jews with the Hp 1-1 phenotype had significantly poorer cognitive function than non-Hp 1-1 in the domains of Attention/Working Memory (p = 0.035) and Executive Function (p = 0.023), but not in Language/Semantic Categorization (p = 0.432), Episodic Memory (p = 0.268), or Overall Cognition (p = 0.082). After controlling for additional covariates (type 2 diabetes-related characteristics, cardiovascular risk factors, Mini-mental State Examination, and extent of depressive symptoms), Attention/Working Memory (p = 0.038) and Executive Function (p = 0.013) remained significant. Older individuals from specific ethnic/cultural backgrounds with the Hp 1-1 phenotype may benefit more from treatment targeted at decreasing or halting the detrimental effects of Hp 1-1 on the brain. Future studies should examine differential associations of Hp 1-1 and cognitive impairment, especially for groups with high prevalence of both, such as African-Americans and Hispanics. Copyright © 2015 John Wiley & Sons, Ltd.

HP-SEE User Forum 2012

CERN Document Server

Karaivanova, Aneta; Oulas, Anastasis; Liabotis, Ioannis; Stojiljkovic, Danica; Prnjat, Ognjen

2014-01-01

This book is a collection of carefully reviewed papers presented during the HP-SEE User Forum, the meeting of the High-Performance Computing Infrastructure for South East Europe’s (HP-SEE) Research Communities, held in October 17-19, 2012, in Belgrade, Serbia. HP-SEE aims at supporting and integrating regional HPC infrastructures; implementing solutions for HPC in the region; and making HPC resources available to research communities in SEE, region, which are working in a number of scientific fields with specific needs for massively parallel execution on powerful computing resources. HP-SEE brings together research communities and HPC operators from 14 different countries and enables them to share HPC facilities, software, tools, data and research results, thus fostering collaboration and strengthening the regional and national human network; the project specifically supports research groups in the areas of computational physics, computational chemistry and the life sciences. The contributions presented i...

Evaluation of HP0605 and HP0971 genes of efflux pumps in Helicobacter pylori resistance to Metronidazole

Directory of Open Access Journals (Sweden)

Mohammad hasan Shirazi

2009-12-01

Full Text Available Background: The presence of antibiotic resistance has been reported in H.pylori and it is a major cause of treatment failure. Five families of multidrug efflux pumps are defined in bacteria and resistance-nodulation-division (RND pumps are found mainly in gram negative bacteria. TolC is one of RND pump components and play a critical role in drug resistance. It hasn’t been established that RND family has a role in drug resistance in H.pylori. In this study, we assessed the role of two efflux genes in resistant to metronidazole in H.pylori by evaluation of overexpression TolC genes by RT-PCR method. Methods: In five metronidazole resistant strains of H.pylori, total RNA was extracted. RNA treated with DNase and RNA reverse transcribed to cDNA. Aliquots of the cDNA solution were assayed by RT- PCR for HP0605 and HP0971 genes. The levels of mRNA expression were evaluated by densitometry analysis. Results: All five strains displayed overexpression for HP0605 basis of increased concentration of metronidazole. Three strains showed transcripts for HP0971. One of these had transcripts for HP0971 only in Metronidazole concentration equaled to 16 µg/ml but two strains overexpressed adapt to increase concentration of metronidazole. Conclusion: According to current study, HP0605 and HP0971 genes overexpressed due to increase metronidazole. So, increasing of Metronidazole affects in H.pylori΄s efflux system in transcription level.

A parallel direct solver for the self-adaptive hp Finite Element Method

KAUST Repository

Paszyński, Maciej R.

2010-03-01

In this paper we present a new parallel multi-frontal direct solver, dedicated for the hp Finite Element Method (hp-FEM). The self-adaptive hp-FEM generates in a fully automatic mode, a sequence of hp-meshes delivering exponential convergence of the error with respect to the number of degrees of freedom (d.o.f.) as well as the CPU time, by performing a sequence of hp refinements starting from an arbitrary initial mesh. The solver constructs an initial elimination tree for an arbitrary initial mesh, and expands the elimination tree each time the mesh is refined. This allows us to keep track of the order of elimination for the solver. The solver also minimizes the memory usage, by de-allocating partial LU factorizations computed during the elimination stage of the solver, and recomputes them for the backward substitution stage, by utilizing only about 10% of the computational time necessary for the original computations. The solver has been tested on 3D Direct Current (DC) borehole resistivity measurement simulations problems. We measure the execution time and memory usage of the solver over a large regular mesh with 1.5 million degrees of freedom as well as on the highly non-regular mesh, generated by the self-adaptive h p-FEM, with finite elements of various sizes and polynomial orders of approximation varying from p = 1 to p = 9. From the presented experiments it follows that the parallel solver scales well up to the maximum number of utilized processors. The limit for the solver scalability is the maximum sequential part of the algorithm: the computations of the partial LU factorizations over the longest path, coming from the root of the elimination tree down to the deepest leaf. © 2009 Elsevier Inc. All rights reserved.

HP investeerib tulevikku / Fredy-Edwin Esse

Index Scriptorium Estoniae

Esse, Fredy-Edwin

2010-01-01

Maailma suurima tehnoloogiafirma Hewlett-Packard (HP) tootearenduskulud kasvasid IV kvartalis 16%, 814 mln dollarini. Autor märgib, et HP aktsia on praegu väga hea saak, kuid mingit imehüpet aktsia hinnas ei tuleks kindlasti oodata

Chemical characterization of outdoor and subway fine (PM(2.5-1.0)) and coarse (PM(10-2.5)) particulate matter in Seoul (Korea) by computer-controlled scanning electron microscopy (CCSEM).

Science.gov (United States)

Byeon, Sang-Hoon; Willis, Robert; Peters, Thomas M

2015-02-13

Outdoor and indoor (subway) samples were collected by passive sampling in urban Seoul (Korea) and analyzed with computer-controlled scanning electron microscopy coupled with energy dispersive x-ray spectroscopy (CCSEM-EDX). Soil/road dust particles accounted for 42%-60% (by weight) of fine particulate matter larger than 1 µm (PM(2.5-1.0)) in outdoor samples and 18% of PM2.5-1.0 in subway samples. Iron-containing particles accounted for only 3%-6% in outdoor samples but 69% in subway samples. Qualitatively similar results were found for coarse particulate matter (PM(10-2.5)) with soil/road dust particles dominating outdoor samples (66%-83%) and iron-containing particles contributing most to subway PM(10-2.5) (44%). As expected, soil/road dust particles comprised a greater mass fraction of PM(10-2.5) than PM(2.5-1.0). Also as expected, the mass fraction of iron-containing particles was substantially less in PM(10-2.5) than in PM(2.5-1.0). Results of this study are consistent with known emission sources in the area and with previous studies, which showed high concentrations of iron-containing particles in the subway compared to outdoor sites. Thus, passive sampling with CCSEM-EDX offers an inexpensive means to assess PM(2.5-1.0) and PM(10-2.5) simultaneously and by composition at multiple locations.

An Entropy Stable h/p Non-Conforming Discontinuous Galerkin Method with the Summation-by-Parts Property

KAUST Repository

Friedrich, Lucas

2017-12-29

This work presents an entropy stable discontinuous Galerkin (DG) spectral element approximation for systems of non-linear conservation laws with general geometric (h) and polynomial order (p) non-conforming rectangular meshes. The crux of the proofs presented is that the nodal DG method is constructed with the collocated Legendre-Gauss-Lobatto nodes. This choice ensures that the derivative/mass matrix pair is a summation-by-parts (SBP) operator such that entropy stability proofs from the continuous analysis are discretely mimicked. Special attention is given to the coupling between nonconforming elements as we demonstrate that the standard mortar approach for DG methods does not guarantee entropy stability for non-linear problems, which can lead to instabilities. As such, we describe a precise procedure and modify the mortar method to guarantee entropy stability for general non-linear hyperbolic systems on h/p non-conforming meshes. We verify the high-order accuracy and the entropy conservation/stability of fully non-conforming approximation with numerical examples.

Physicochemical properties of calcium silicate-based formulations MTA Repair HP and MTA Vitalcem.

Science.gov (United States)

Guimarães, Bruno Martini; Prati, Carlo; Duarte, Marco Antonio Hungaro; Bramante, Clovis Monteiro; Gandolfi, Maria Giovanna

2018-04-05

This study aimed to analyze the following physicochemical properties: radiopacity, final setting time, calcium release, pH change, solubility, water sorption, porosity, surface morphology, and apatite-forming ability of two calcium silicate-based materials. We tested MTA Repair HP and MTA Vitalcem in comparison with conventional MTA, analyzing radiopacity and final setting time. Water absorption, interconnected pores and apparent porosity were measured after 24-h immersion in deionized water at 37°C. Calcium and pH were tested up to 28 d in deionized water. We analyzed data using two-way ANOVA with Student-Newman-Keuls tests (pcalcium release at 28 d (pcalcium phosphate on their surface after 28 d in HBSS. MTA Repair HP and MTA Vitalcem had extended alkalinizing activity and calcium release that favored calcium phosphate nucleation. The presence of the plasticizer in MTA HP might increase its solubility and porosity. The radiopacifier calcium tungstate can be used to replace bismuth oxide.

HP-multigrid as smoother algorithm for higher order discontinuous Galerkin discretizations of advection dominated flows. Part I. Multilevel Analysis

NARCIS (Netherlands)

van der Vegt, Jacobus J.W.; Rhebergen, Sander

2011-01-01

The hp-Multigrid as Smoother algorithm (hp-MGS) for the solution of higher order accurate space-(time) discontinuous Galerkin discretizations of advection dominated flows is presented. This algorithm combines p-multigrid with h-multigrid at all p-levels, where the h-multigrid acts as smoother in the

HP advances Grid Strategy for the adaptive enterprise

CERN Multimedia

2003-01-01

"HP today announced plans to further enable its enterprise infrastructure technologies for grid computing. By leveraging open grid standards, HP plans to help customers simplify the use and management of distributed IT resources. The initiative will integrate industry grid standards, including the Globus Toolkit and Open Grid Services Architecture (OGSA), across HP's enterprise product lines" (1 page).

The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage.

Science.gov (United States)

Leonard, Phoebe H; Grzenda, Adrienne; Mathison, Angela; Morbeck, Dean E; Fredrickson, Jolene R; de Assuncao, Thiago M; Christensen, Trace; Salisbury, Jeffrey; Calvo, Ezequiel; Iovanna, Juan; Coddington, Charles C; Urrutia, Raul; Lomberk, Gwen

2015-05-29

HP1γ, a well-known regulator of gene expression, has been recently identified to be a target of Aurora A, a mitotic kinase which is important for both gametogenesis and embryogenesis. The purpose of this study was to define whether the Aurora A-HP1γ pathway supports cell division of gametes and/or early embryos, using western blot, immunofluorescence, immunohistochemistry, electron microscopy, shRNA-based knockdown, site-directed mutagenesis, and Affymetrix-based genome-wide expression profiles. We find that the form of HP1γ phosphorylated by Aurora A, P-Ser83 HP1γ, is a passenger protein, which localizes to the spermatozoa centriole and axoneme. In addition, disruption in this pathway causes centrosomal abnormalities and aberrations in cell division. Expression profiling of male germ cell lines demonstrates that HP1γ phosphorylation is critical for the regulation of mitosis-associated gene expression networks. In female gametes, we observe that P-Ser83-HP1γ is not present in meiotic centrosomes of M2 oocytes, but after syngamy, it becomes detectable during cleavage divisions, coinciding with early embryonic genome activation. These results support the idea that phosphorylation of HP1γ by Aurora A plays a role in the regulation of gene expression and mitotic cell division in cells from the sperm lineage and in early embryos. Combined, this data is relevant to better understanding the function of HP1γ in reproductive biology.

Celiac disease and Hp infection association in Iran Enfermedades celiacas e infección por Hp en Irán

Directory of Open Access Journals (Sweden)

M. Rostami-Nejad

2009-12-01

Full Text Available Background: we assessed the prevalence, the related symptoms, and the endoscopic and histologic gastric features of celiac disease (CD in patients with Helicobacter pylori (Hp. Methods: 450 dyspeptic patients were studied. Biopsies of gastric antrum and duodenum, CD serology, and total IgA were obtained. Histological findings were scored with the Marsh-Rostami criteria. Results: 411 (91.3% patients were Hp positive. Duodenal histology was normal in 385 (85.6% patients, 124 (27.5% had duodenitis and 28 (6.2% showed duodenal abnormalities (Marsh I-IIIc. Twenty three/28 (82.1% patients with malabsorption pattern were also Hp positive. Serological analysis: 12 of 31 (38.7% positive patients had abnormal histology (Marsh I,-IIIc. Nine out 450 patients were IgA deficient; none of them was serologically positive for CD. Conclusion: although a high prevalence of Hp infection was found in this study, the relationship between Hp infection and CD was similar to that reported in other geographic areas.

Goal-Oriented Self-Adaptive hp Finite Element Simulation of 3D DC Borehole Resistivity Simulations

KAUST Repository

Calo, Victor M.

2011-05-14

In this paper we present a goal-oriented self-adaptive hp Finite Element Method (hp-FEM) with shared data structures and a parallel multi-frontal direct solver. The algorithm automatically generates (without any user interaction) a sequence of meshes delivering exponential convergence of a prescribed quantity of interest with respect to the number of degrees of freedom. The sequence of meshes is generated from a given initial mesh, by performing h (breaking elements into smaller elements), p (adjusting polynomial orders of approximation) or hp (both) refinements on the finite elements. The new parallel implementation utilizes a computational mesh shared between multiple processors. All computational algorithms, including automatic hp goal-oriented adaptivity and the solver work fully in parallel. We describe the parallel self-adaptive hp-FEM algorithm with shared computational domain, as well as its efficiency measurements. We apply the methodology described to the three-dimensional simulation of the borehole resistivity measurement of direct current through casing in the presence of invasion.

Quantitation of anticonvulsant drugs in serum by gas-chromatography on the stationary phase SP-2510.

Science.gov (United States)

Godolphin, W; Thoma, J

1978-03-01

A new column packing, SP-2510 DA (Supelco, Inc., Bellefonte, Pa. 16823), is an excellent stationary phase for the determination of a wide variety of anticonvulsant drugs by gas--liquid chromatography without derivatization. However, when uncomplicated extraction procedures are used, serum cholesterol interferes with the determination of primidone. By the simple expedient of adding a short "pre-column" containing another phase (SP-2250 DA) the problem is overcome.

Analysis of HP1α regulation in human breast cancer cells

DEFF Research Database (Denmark)

Thomsen, Rune; Christensen, Dennis B; Rosborg, Sanne

2011-01-01

The three mammalian HP1 proteins, HP1α/CBX5, HP1β/CBX1, and HPγ/CBX3, are involved in chromatin packing and gene regulation. The HP1α protein is down-regulated in invasive compared to non-invasive breast cancer cells and HP1α is a suppressor of cell migration and invasion. In this report, we...... examined the background for HP1α protein down-regulation in invasive breast cancer cells. We identified a strict correlation between HP1α down-regulation at the protein level and the mRNA level. The HP1α mRNA down-regulation in invasive cancer cells was not caused by mRNA destabilization. Chromatin...... immunoprecipitation analysis of the HP1α gene showed a decrease in the histone mark for transcriptional activity H3-K36 tri-methylation and RNA polymerase II in invasive breast cancer cells which correlated with a decreased abundance of basal transcription factors at the HP1α promoter. E2F transcription factors...

Studying the Efficiency of XAD-7HP Anionic Resin in the Extraction of Humic Acid from Surface Water

Directory of Open Access Journals (Sweden)

Hassan Hashemi

2017-09-01

Results: The average extraction efficiency for XAD-7HP was 77% and HA concentration determined in all seasons ranges from 0.15 to 0.29 mg/L in raw water. Conclusions: From the findings of this study, it can be concluded that the use of XAD-7HP is a proper and effective method for the extraction of HS and its extraction efficiency is more influenced by pH and turbidity than other parameters. It can also be deduced from this study that XAD-7HP resin was most efficient at a pH of 2 and an increase in turbidity resulted in a corresponding decrease in the efficiency of resin.

Interaction of HP1 and Brg1/Brm with the globular domain of histone H3 is required for HP1-mediated repression.

Directory of Open Access Journals (Sweden)

Marc Lavigne

2009-12-01

Full Text Available The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. These proteins contain two related domains known as the chromo- and the chromoshadow-domain. The chromo-domain binds histone H3 tails methylated on lysine 9. However, in vivo and in vitro experiments have shown that the affinity of HP1 proteins to native methylated chromatin is relatively poor and that the opening of chromatin occurring during DNA replication facilitates their binding to nucleosomes. These observations prompted us to investigate whether HP1 proteins have additional histone binding activities, envisioning also affinity for regions potentially occluded by the nucleosome structure. We find that the chromoshadow-domain interacts with histone H3 in a region located partially inside the nucleosomal barrel at the entry/exit point of the nucleosome. Interestingly, this region is also contacted by the catalytic subunits of the human SWI/SNF complex. In vitro, efficient SWI/SNF remodeling requires this contact and is inhibited in the presence of HP1 proteins. The antagonism between SWI/SNF and HP1 proteins is also observed in vivo on a series of interferon-regulated genes. Finally, we show that SWI/SNF activity favors loading of HP1 proteins to chromatin both in vivo and in vitro. Altogether, our data suggest that HP1 chromoshadow-domains can benefit from the opening of nucleosomal structures to bind chromatin and that HP1 proteins use this property to detect and arrest unwanted chromatin remodeling.

Comparison of four urease-dependent tests of detecting HP infection

International Nuclear Information System (INIS)

Han Yuan; Huang Gang; Zhu Cuiying; Ling Liqun

2001-01-01

Objective: The aim of this study is to compare the four urease-dependent tests of detecting Hp infection. The authors used following method: 1) rapid urease test (RUT), 2) 14 C urea breath test ( 14 C-UBT), 3) 13 C urea breath test which is measured by with infrared spectrometry ( 13 C-UBT1), 4) 13 C urea breath test with mass spectrometry ( 13 C-UBT). Methods: 54 patients underwent RUT, 14 C-UBT, 13 C-UBT1, 13 C-UBT2 respectively. Results: Sensitivity and specificity, accurate rate, positive predictive value (PPV), negative predictive value (NPV), respectively, were as follows: RUT (82.1%, 80%, 81.5%, 91.4%, 63.2%), 14 C-UBT (97.4%, 100%, 98.1%, 100%, 93.8%), 13 C-UBT1 (100%, 93.3%, 98.1%, 97.5%, 100%), 13 C-UBT2 (100%, 86.7%, 96.3%, 95.1%, 100%), PPV of four tests are not significantly different (P>0.05). Conclusion: Four urease-dependent tests are high sensitive and accurate methods for detecting Hp infection. UBT is an ideal tool for diagnosis, screen and follow-up of Hp infection

Growth, extracellular alkaline phosphatase activity, and kinetic characteristic responses of the bloom-forming toxic cyanobacterium, Microcystis aeruginosa, to atmospheric particulate matter (PM2.5, PM2.5-10, and PM>10).

Science.gov (United States)

Xu, Ziran; Wang, Shoubing; Wang, Yuanan; Zhang, Jie

2018-03-01

Atmospheric particulate matter (APM), commonly seen and widely excited in environment, appears great enough to influence the biochemical processes in aquatic microorganisms and phytoplankton. Understanding the response of cyanobacteria to various factors is fundamental for eutrophication control. To clarify the response of cyanobacteria to APM, the effects of PM 2.5 , PM 2.5-10 , and PM >10 on Microcystis aeruginosa were researched. Variabilities in cell density, chlorophyll a, soluble protein, malondialdehyde, extracellular activity, and kinetic parameters of alkaline phosphatase were evaluated by lab-cultured experiments. Results showed that the PM 2.5 had a slight stimulation impact on the growth and enhanced both of the 48- and 72-h extracellular alkaline phosphatase activity (APA), the affinity of alkaline phosphatase for substrate, and the 72-h maximum enzymatic reaction velocity (V max ). Moreover, the stimulations in extracellular APA and V max enhanced with the increasing exposure concentrations. We also found there were no obvious distinctions on the effects of growth and alkaline phosphatase in M. aeruginosa between PM 2.5-10 and PM >10 exposure groups. Obviously, inhibitory effects on growth existed in 4.0 and 8.0 mg/L PM 2.5-10 and 8.0 mg/L PM >10 at 120 h. Furthermore, PM 2.5-10 and PM >10 exerted inhibitory effects on the extracellular APA during the 72-h exposure. Simultaneously, the V max was notably inhibited and the affinity of alkaline phosphatase for substrate was more inseparable compared with control in PM 2.5-10 and PM >10 treatments. Nevertheless, the inhibitors in extracellular APA and kinetic parameters were unrelated to PM 2.5-10 and PM >10 exposure concentrations. Two-way ANOVA results revealed that there were significant interactions between exposure concentration and diameter of APM on the 120-h cell density, soluble protein content, APA, and 72 h APA of M. aeruginosa. These results in our study would be meaningful to further

hp Spectral element methods for three dimensional elliptic problems

Indian Academy of Sciences (India)

This is the first of a series of papers devoted to the study of h-p spec- .... element functions defined on mesh elements in the new system of variables with a uni- ... the spectral element functions on these elements and give construction of the stability .... By Hm( ), we denote the usual Sobolev space of integer order m ≥ 0 ...

The Effect of Replacing Fish Meal in the Diet with Enzyme-Treated Soybean Meal (HP310) on Growth and Body Composition of Rainbow Trout Fry.

Science.gov (United States)

Haghbayan, Samira; Shamsaie Mehrgan, Mehdi

2015-11-26

The potential of enzyme-treated soybean meal powder (HP310) as fish meal alternative in diets for rainbow trout weighing 1.17 ± 0.3 g was evaluated for 60 days. Fish meal was replaced with HP310 at 25%, 50%, 75% and 100% of experimental diets. A control group was also considered. The results showed that diets containing 75% and 100% HP310 had significantly higher feed conversion ratio and lower feed intake, weight gain and specific growth rate compared to fish feed diets containing higher levels of fish protein ingredients (p replacement levels of diet (p > 0.05). However increasing in level of HP310 in the diet caused a significant increase of the white blood cells (p replaced by HP310 showed the highest values of ash and moisture content among the diets and showed significantly different levels when compared with the control and other feeding treatments (p < 0.05).

Metamorphic P-T path and zircon U-Pb dating of HP mafic granulites in the Yushugou granulite-peridotite complex, Chinese South Tianshan, NW China

Science.gov (United States)

Zhang, Lu; Zhang, Lifei; Xia, Bin; Lü, Zeng

2018-03-01

Co-existing granulite and peridotite may represent relics of the paleo-suture zone and provides an optimal opportunity for better understanding of orogeny between two blocks. In this study, we carried out petrological and U-Pb zircon dating investigation on the HP mafic granulites associated with peridotite complex at Yushugou in Chinese South Tianshan. The studied samples include garnet-bearing high-pressure mafic granulites which can be subdivided into two types: Type I orthopyroxene-free and Type II orthopyroxene-bearing granulites and amphibolite. Type I granulite (Y21-2) has a mineral assemblage of garnet (33 vol.%), clinopyroxene (32 vol.%) and plagioclase (30 vol.%); and Type II granulite (Y18-8) has a mineral assemblage of garnet (22 vol.%), clinopyroxene (10 vol.%), orthopyroxene (14 vol.%), plagioclase (45 vol.%) and quartz. Garnet in both granulites exhibits core-rim structure characterized by increasing grossular and decreasing pyrope from core to rim. Petrographic observations and phase equilibrium modeling using THERMOCALC in the NCFMASHTO system for the mafic granulites (Y21-2 and Y18-8) show three stages of metamorphism: Stage I (granulite facies) was recognized by the large porphyroblastic garnet core, with P-T conditions of 9.8-10.4 Kbar and 860-900 °C (Y21-2) and 9.9-10.6 Kbar and 875-890 °C (Y18-8), respectively; Stage II (HP granulite facies) has peak P-T conditions of 12.1 Kbar at 755 °C (Y21-2) and 13.8 Kbar at 815 °C (Y18-8) using mineral assemblages combining with garnet rim compositions with maximum grossular and minimum pyrope contents; Stage III (amphibolite facies) was characterized by the development of calcic amphibole in granulites with temperature of 446-563 °C. Therefore, an anticlockwise P-T path characterized by simultaneous temperature-decreasing and pressure-increasing was inferred for the Yushugou HP mafic granulite. Studies of zircon morphology and inclusions, combined with zircon U-Pb dating and REE geochemistry

An object-oriented decomposition of the adaptive-hp finite element method

Energy Technology Data Exchange (ETDEWEB)

Wiley, J.C.

1994-12-13

Adaptive-hp methods are those which use a refinement control strategy driven by a local error estimate to locally modify the element size, h, and polynomial order, p. The result is an unstructured mesh in which each node may be associated with a different polynomial order and which generally require complex data structures to implement. Object-oriented design strategies and languages which support them, e.g., C++, help control the complexity of these methods. Here an overview of the major classes and class structure of an adaptive-hp finite element code is described. The essential finite element structure is described in terms of four areas of computation each with its own dynamic characteristics. Implications of converting the code for a distributed-memory parallel environment are also discussed.

Spatially adaptive hp refinement approach for PN neutron transport equation using spectral element method

International Nuclear Information System (INIS)

Nahavandi, N.; Minuchehr, A.; Zolfaghari, A.; Abbasi, M.

2015-01-01

Highlights: • Powerful hp-SEM refinement approach for P N neutron transport equation has been presented. • The method provides great geometrical flexibility and lower computational cost. • There is a capability of using arbitrary high order and non uniform meshes. • Both posteriori and priori local error estimation approaches have been employed. • High accurate results are compared against other common adaptive and uniform grids. - Abstract: In this work we presented the adaptive hp-SEM approach which is obtained from the incorporation of Spectral Element Method (SEM) and adaptive hp refinement. The SEM nodal discretization and hp adaptive grid-refinement for even-parity Boltzmann neutron transport equation creates powerful grid refinement approach with high accuracy solutions. In this regard a computer code has been developed to solve multi-group neutron transport equation in one-dimensional geometry using even-parity transport theory. The spatial dependence of flux has been developed via SEM method with Lobatto orthogonal polynomial. Two commonly error estimation approaches, the posteriori and the priori has been implemented. The incorporation of SEM nodal discretization method and adaptive hp grid refinement leads to high accurate solutions. Coarser meshes efficiency and significant reduction of computer program runtime in comparison with other common refining methods and uniform meshing approaches is tested along several well-known transport benchmarks

Clinical value of 13C-UBT diagnosing infection of the Hp

International Nuclear Information System (INIS)

Xu Changde; Chen Shaoliang; Liu Wenguan

2004-01-01

100 dyspeptic patients are performed an endoscopy with biopsy for histology, rapid urease test, 13 C-urea breath test( 13 C-UBT) and ASSURE TM Hp rapid Test(HpRT). Patients are considered to be infected if both histology and biopsies yield positive results, and not infected when both tests are negative. The pathology and persons responsible for endoscopy, urease test and 13 C-UBT are unaware of the results from the other diagnostic methods. The 13 C-UBT has the following results: sensitivity 96% ,specificity 95%; rapid urease test has the following results: sensitivity 79%, specificity 76%; HpRT has the following results: sensitivity 86%, specificity 88%. 13 C-UBT provides excellent sensitivity and specificity for the diagnosis of Hp. Pylori infected Hp, it can be the first choice for detecting the infection of the Hp. (authors)

PM 2.5-10, PM 2.5 and associated water-soluble inorganic species at a coastal urban site in the metropolitan region of Rio de Janeiro

Science.gov (United States)

Mariani, Rauda L.; de Mello, William Z.

The concentrations of PM 2.5-10, PM 2.5 and associated water-soluble inorganic species (WSIS) were determined in a coastal site of the metropolitan region of Rio de Janeiro, Southeastern Brazil, from October 1998 to September 1999 ( n=50). Samples were dissolved in water and analyzed for major inorganic ions. The mean (± standard deviation; median) concentrations of PM 2.5-10 and PM 2.5 were, respectively, 26 (± 16; 21) μg m -3 and 17 (± 13; 14) μg m -3. Their mean concentrations were 1.7-1.8 times higher in dry season (May-October) than in rainy season (November-April). The WSIS comprised, respectively, 34% and 28% of the PM 2.5-10 and PM 2.5 masses. Chloride, Na + and Mg 2+ were the predominant ions in PM 2.5-10, indicating a significant influence of sea-salt aerosols. In PM 2.5, SO 42- (˜97% nss-SO 42-) and NH 4+ were the most abundant ions and their equivalent concentration ratio (SO 42-/NH 4+ ˜1.0) suggests that they were present as (NH 4) 2SO 4 particles. The mean concentration of (NH 4) 2SO 4 was 3.4 μg m -3. The mean equivalent PM 2.5 NO 3- concentration was eight times smaller than those of SO 42- and NH 4+. The PM 2.5 NO 3- concentration in dry season was three times higher than in rainy season, probably due to reaction of NaCl (sea salt) with HNO 3 as a result of higher levels of NO y during the dry season and/or reduced volatilization of NH 4NO 3 due to lower wintertime temperature. Chloride depletion was observed in both size ranges, although more pronouncely in PM 2.5.

Effects of a low-energy proton irradiation on n+/p-AlInGaP solar cells

International Nuclear Information System (INIS)

Lee, H.S.; Yamaguchi, M.; Ekins-Daukes, N.J.; Khan, A.; Takamoto, T.; Imaizumi, M.; Ohshima, T.; Itoh, H.

2006-01-01

For the first time, by deep-level transient spectroscopy, 30keV proton irradiation-induced defects in n + /p-AlInGaP solar cells have been observed. After the 30keV proton irradiation, new deep-level defects such as two majority-carrier (hole) traps HP1 (E V +0.98eV, N T =3.8x10 14 cm -3 ) and HP2, and two minority-carrier (electron) traps EP1 (E C -0.71eV, N T =2.0x10 15 cm -3 ) and EP2 have been observed in p-AlInGaP. The introduction rate of majority-carrier trap center (HP1) is 380cm -1 , which is lower than that (1500cm -1 ) in 100keV proton-irradiated p-InGaP. From the minority-carrier injection annealing for HP1 defect and carrier concentration in 30keV proton-irradiated p-AlInGaP, HP1 defect is likely to act as a recombination center as well as a compensator center

HP Ge planar detectors

International Nuclear Information System (INIS)

Gornov, M.G.; Gurov, Yu.B.; Soldatov, A.M.; Osipenko, B.P.; Yurkowski, J.; Podkopaev, O.I.

1989-01-01

Parameters of planar detectors manufactured of HP Ge are presented. The possibilities to use multilayer spectrometers on the base of such semiconductor detectors for nuclear physics experiments are discussed. It is shown that the obtained detectors including high square ones have spectrometrical characteristics close to limiting possible values. 9 refs.; 3 figs.; 1 tab

Development of Hybrid pH sensor for long-term seawater pH monitoring.

Science.gov (United States)

Nakano, Y.; Egashira, T.; Miwa, T.; Kimoto, H.

2016-02-01

We have been developing the in situ pH sensor (Hybrid pH sensor: HpHS) for the long-term seawater pH monitoring. We are planning to provide the HpHS for researchers and environmental consultants for observation of the CCS (Carbon dioxide Capture and Storage) monitoring system, the coastal environment monitoring system (e.g. Blue Carbon) and ocean acidification. The HpHS has two types of pH sensors (i.e. potentiometric pH sensor and spectrophotometric pH sensor). The spectrophotometric pH sensor can measure pH correctly and stably, however it needs large power consumption and a lot of reagents in a long period of observation. The pH sensor used m-cresol purple (mCP) as an indicator of pH (Clayton and Byrne, 1993 and Liu et al., 2011). We can choose both coefficients before deployment. On the other hand, although the potentiometric pH sensor is low power consumption and high-speed response (within 10 seconds), drifts in the pH of the potentiometric measurements may possibly occur for a long-term observation. The HpHS can measure in situ pH correctly and stably combining advantage of both pH sensors. The HpHS consists of an aluminum pressure housing with optical cell (main unit) and an aluminum silicon-oil filled, pressure-compensated vessel containing pumps and valves (diaphragm pump and valve unit) and pressure-compensated reagents bags (pH indicator, pure water and Tris buffer or certified reference material: CRM) with an ability to resist water pressure to 3000m depth. The main unit holds system control boards, pump drivers, data storage (micro SD card), LED right source, photodiode, optical cell and pressure proof windows. The HpHS also has an aluminum pressure housing that holds a rechargeable lithium-ion battery or a lithium battery for the power supply (DC 24 V). The HpHS is correcting the value of the potentiometric pH sensor (measuring frequently) by the value of the spectrophotometric pH sensor (measuring less frequently). It is possible to calibrate in

Investigating tumor perfusion and metabolism using multiple hyperpolarized 13C compounds: HP001, pyruvate and urea

DEFF Research Database (Denmark)

von Morze, Cornelius; Larson, Peder E.Z.; Hu, Simon

2012-01-01

The metabolically inactive hyperpolarized agents HP001 (bis-1,1-(hydroxymethyl)-[1-13C]cyclopropane-d8) and urea enable a new type of perfusion magnetic resonance imaging based on a direct signal source that is background-free. The addition of perfusion information to metabolic information obtained...... (T1=95 s ex vivo, 32 s in vivo at 3 T) using a pulse sequence with balanced steady-state free precession and ramped flip angle over time for efficient utilization of the hyperpolarized magnetization and three-dimensional echo-planar spectroscopic imaging of urea copolarized with [1-13C...... of separate dynamic HP001 imaging and copolarized pyruvate/urea imaging were compared. A strong and significant correlation (R=0.73, P=.02) detected between the urea and HP001 data confirmed the value of copolarizing urea with pyruvate for simultaneous assessment of perfusion and metabolism....

High-resolution mapping reveals links of HP1 with active and inactive chromatin components.

Directory of Open Access Journals (Sweden)

Elzo de Wit

2007-03-01

Full Text Available Heterochromatin protein 1 (HP1 is commonly seen as a key factor of repressive heterochromatin, even though a few genes are known to require HP1-chromatin for their expression. To obtain insight into the targeting of HP1 and its interplay with other chromatin components, we have mapped HP1-binding sites on Chromosomes 2 and 4 in Drosophila Kc cells using high-density oligonucleotide arrays and the DNA adenine methyltransferase identification (DamID technique. The resulting high-resolution maps show that HP1 forms large domains in pericentric regions, but is targeted to single genes on chromosome arms. Intriguingly, HP1 shows a striking preference for exon-dense genes on chromosome arms. Furthermore, HP1 binds along entire transcription units, except for 5' regions. Comparison with expression data shows that most of these genes are actively transcribed. HP1 target genes are also marked by the histone variant H3.3 and dimethylated histone 3 lysine 4 (H3K4me2, which are both typical of active chromatin. Interestingly, H3.3 deposition, which is usually observed along entire transcription units, is limited to the 5' ends of HP1-bound genes. Thus, H3.3 and HP1 are mutually exclusive marks on active chromatin. Additionally, we observed that HP1-chromatin and Polycomb-chromatin are nonoverlapping, but often closely juxtaposed, suggesting an interplay between both types of chromatin. These results demonstrate that HP1-chromatin is transcriptionally active and has extensive links with several other chromatin components.

Differentiation-specific association of HP1alpha and HP1beta with chromocentres is correlated with clustering of TIF1beta at these sites

Czech Academy of Sciences Publication Activity Database

Bártová, Eva; Pacherník, J.; Kozubík, Alois; Kozubek, Stanislav

2007-01-01

Roč. 127, č. 4 (2007), s. 375-388 ISSN 0948-6143 R&D Projects: GA AV ČR(CZ) 1QS500040508; GA AV ČR(CZ) IAA5004306; GA MŠk(CZ) LC535; GA ČR(CZ) GA204/06/0978; GA ČR(CZ) GP524/03/P171 Institutional research plan: CEZ:AV0Z50040702 Keywords : HP1 proteiny * TIF1beta * chromocentry Subject RIV: BO - Biophysics Impact factor: 2.893, year: 2007

THE EFFECTS OF DIFFERENT K2HP04 AND NaCl LEVELS ON THE WATER-HOLDING CAPACITY AND COOKING LOSS OF GOAT MEAT

Directory of Open Access Journals (Sweden)

Mustafa Karakaya

1996-01-01

Full Text Available This research was conducted on the laboratory conditions. Different levels of K2HP04 (0.00%, 0.25%, 0.30% and NaCl (2.5%, 3.0% were applied on the goat meat and the pH, water holding capacity and cooking loss were observed. According to the results the effects of 0.25% K2HP04 addition was found statistically insignificant (p

A nonlinear HP-type complementary resistive switch

Directory of Open Access Journals (Sweden)

Paul K. Radtke

2016-05-01

Full Text Available Resistive Switching (RS is the change in resistance of a dielectric under the influence of an external current or electric field. This change is non-volatile, and the basis of both the memristor and resistive random access memory. In the latter, high integration densities favor the anti-serial combination of two RS-elements to a single cell, termed the complementary resistive switch (CRS. Motivated by the irregular shape of the filament protruding into the device, we suggest a nonlinearity in the resistance-interpolation function, characterized by a single parameter p. Thereby the original HP-memristor is expanded upon. We numerically simulate and analytically solve this model. Further, the nonlinearity allows for its application to the CRS.

A nonlinear HP-type complementary resistive switch

Science.gov (United States)

Radtke, Paul K.; Schimansky-Geier, Lutz

2016-05-01

Resistive Switching (RS) is the change in resistance of a dielectric under the influence of an external current or electric field. This change is non-volatile, and the basis of both the memristor and resistive random access memory. In the latter, high integration densities favor the anti-serial combination of two RS-elements to a single cell, termed the complementary resistive switch (CRS). Motivated by the irregular shape of the filament protruding into the device, we suggest a nonlinearity in the resistance-interpolation function, characterized by a single parameter p. Thereby the original HP-memristor is expanded upon. We numerically simulate and analytically solve this model. Further, the nonlinearity allows for its application to the CRS.

Cumulative nucleon production in 3Hep- and 3Hp interactions at momenta of colliding nuclei 5 GeV/c

International Nuclear Information System (INIS)

Abdullin, S.K.; Blinov, A.V.; Vanyushin, I.A.

1989-01-01

Inclusive cross sections of cumulative protons produced in 3 Hep- and 3 Hp-interactions under momenta of colliding 5 GeV/c nuclei are investigated. Experimental material is obtained using liquid-hydrogen ITEP chamber-80cm. Under the cumulative proton kinetic energy, exceeding 50 MeV inclusive cross section ratio σ( 3 Hep→pX)/σ( 3 Hp→pX)=1.6±0.1. Within the same energy interval evaluation of yield ratio of cumulative protons and neutrons, produced in 3 Hep -interactions, leads to ∼ 1.6 value. Asymmetry of mean multiple protons escaping forward and backward both in 3 Hep -and in 3 Hp interactions is observed. Averaged invariant distribution functions for cumulative protons and neutrons in 8 Hep-interactions and protons in 3 Hp-interactions are constructed (the averaging is performed within 90-180 deg and 120-180 deg intervals). To temperatures of such distributions are found. 43 refs.; 1 fig

Discrete conservation of nonnegativity for elliptic problems solved by the hp-FEM

Czech Academy of Sciences Publication Activity Database

Šolín, P.; Vejchodský, Tomáš; Araiza, R.

2007-01-01

Roč. 76, 1-3 (2007), s. 205-210 ISSN 0378-4754 R&D Projects: GA ČR GP201/04/P021 Institutional research plan: CEZ:AV0Z10190503 Keywords : discrete nonnegativity conservation * discrete Green's function * elliptic problems * hp-FEM * higher-order finite element methods * Poisson equation * numerical experimetns Subject RIV: BA - General Mathematics Impact factor: 0.738, year: 2007

Optimal design of PV and HP system

DEFF Research Database (Denmark)

Nepper-Rasmussen, Bjarke Christian; Rasmussen, Theis Bo

2015-01-01

Methods of utilizing residential produced photovoltaic (PV) power by converting to thermal energy through heat pumps (HP) are present in literature, where thermal energy is dispersed as either heat or hot water at the instant moment of PV production. In this paper an alternative solution is descr...... that the thermal storage with a BT is a better investment than a PV system without HP or no investment. Furthermore, it showed that the optimization model developed in this project is capable of finding the optimal combination of component sizes based on our data.......Methods of utilizing residential produced photovoltaic (PV) power by converting to thermal energy through heat pumps (HP) are present in literature, where thermal energy is dispersed as either heat or hot water at the instant moment of PV production. In this paper an alternative solution...... is described, where the thermal energy is stored in a buffer tank (BT) capable of dispersing heat to either the heating system of a house or a hot water tank, for later use. The thermal storage solution including a BT can increase the self-consumption of residentially produced PV power and thereby shift...

Concurrent proinflammatory and apoptotic activity of a Helicobacter pylori protein (HP986 points to its role in chronic persistence.

Directory of Open Access Journals (Sweden)

Ayesha Alvi

Full Text Available Helicobacter pylori induces cytokine mediated changes in gastroduodenal pathophysiology, wherein, the activated macrophages at the sub-mucosal space play a central role in mounting innate immune response against the antigens. The bacterium gains niche through persistent inflammation and local immune-suppression causing peptic ulcer disease or chronic gastritis; the latter being a significant risk factor for the development of gastric adenocarcinoma. What favors persistence of H. pylori in the gastric niches is not clearly understood. We report detailed characterization of a functionally unknown gene (HP986, which was detected in patient isolates associated with peptic ulcer and gastric carcinoma. Expression and purification of recombinant HP986 (rHP986 revealed a novel, ∼29 kDa protein in biologically active form which associates with significant levels of humoral immune responses in diseased individuals (p<0.001. Also, it induced significant levels of TNF-α and Interleukin-8 in cultured human macrophages concurrent to the translocation of nuclear transcription factor-κB (NF-κB. Further, the rHP986 induced apoptosis of cultured macrophages through a Fas mediated pathway. Dissection of the underlying signaling mechanism revealed that rHP986 induces both TNFR1 and Fas expression to lead to apoptosis. We further demonstrated interaction of HP986 with TNFR1 through computational and experimental approaches. Independent proinflammatory and apoptotic responses triggered by rHP986 as shown in this study point to its role, possibly as a survival strategy to gain niche through inflammation and to counter the activated macrophages to avoid clearance.

Microscopic calculation of the molecular-nuclear D+D → sup(3)He+n and D+D → sup(3)H+p reactions at close to zero energies

International Nuclear Information System (INIS)

Frederico, T.; Groote, J.J. de; Hornos, J.E.; Hussein, M.S.

1990-11-01

Microscopic calculations of the astrophysically interesting reactions D+D→sup(3)He+n and D+D→sup(3)H+p are performed using nuclear reaction theory and Born-Oppenheimer type molecular calculation of the D+D initial stage. The sensitivity of the fusion rate to the behaviour of the D+D wave function at close to zero separation is assessed. Relevance of the results to the cold fusion problem is discussed. (author)

Informative gene selection using Adaptive Analytic Hierarchy Process (A2HP

Directory of Open Access Journals (Sweden)

Abhishek Bhola

2017-12-01

Full Text Available Gene expression dataset derived from microarray experiments are marked by large number of genes, which contains the gene expression values at different sample conditions/time-points. Selection of informative genes from these large datasets is an issue of major concern for various researchers and biologists. In this study, we propose a gene selection and dimensionality reduction method called Adaptive Analytic Hierarchy Process (A2HP. Traditional analytic hierarchy process is a multiple-criteria based decision analysis method whose result depends upon the expert knowledge or decision makers. It is mainly used to solve the decision problems in different fields. On the other hand, A2HP is a fused method that combines the outcomes of five individual gene selection ranking methods t-test, chi-square variance test, z-test, wilcoxon test and signal-to-noise ratio (SNR. At first, the preprocessing of gene expression dataset is done and then the reduced number of genes obtained, will be fed as input for A2HP. A2HP utilizes both quantitative and qualitative factors to select the informative genes. Results demonstrate that A2HP selects efficient number of genes as compared to the individual gene selection methods. The percentage of deduction in number of genes and time complexity are taken as the performance measure for the proposed method. And it is shown that A2HP outperforms individual gene selection methods.

Notes on HP1 a software package for simulating variably-saturated water flow, heat transport, solute transport, and biogeochemistry in porous media. HP1 Version 2.2

Energy Technology Data Exchange (ETDEWEB)

Jacques, D.; Simunek, J.

2010-01-15

HP1 is a comprehensive modeling tool in terms of processes and reactions for simulating reactive transport and biogeochemical processes in variably-saturated porous media. HP1 results from coupling the water and solute transport model HYDRUS-1D (Simunek et al., 2009a) and PHREEQC-2 (Parkhurst and Appelo, 1999). This note provides an overview of how to set up and execute a HP1 project using version 2.2.002 of HP1 and version 4.13 of the graphical user interface (GUI) of HYDRUS-1D. A large part of this note are step-by-step instructions for selected examples involving mineral dissolution and precipitation, cation exchange, surface complexation and kinetic degradation networks. The implementation of variably-saturated flow conditions, changing boundary conditions, a layered soil profile or immobile water is also illustrated.

Notes on HP1 a software package for simulating variably-saturated water flow, heat transport, solute transport, and biogeochemistry in porous media. HP1 Version 2.2

International Nuclear Information System (INIS)

Jacques, D.; Simunek, J.

2010-01-01

HP1 is a comprehensive modeling tool in terms of processes and reactions for simulating reactive transport and biogeochemical processes in variably-saturated porous media. HP1 results from coupling the water and solute transport model HYDRUS-1D (Simunek et al., 2009a) and PHREEQC-2 (Parkhurst and Appelo, 1999). This note provides an overview of how to set up and execute a HP1 project using version 2.2.002 of HP1 and version 4.13 of the graphical user interface (GUI) of HYDRUS-1D. A large part of this note are step-by-step instructions for selected examples involving mineral dissolution and precipitation, cation exchange, surface complexation and kinetic degradation networks. The implementation of variably-saturated flow conditions, changing boundary conditions, a layered soil profile or immobile water is also illustrated.

Helicobacter pylori antigen HP0986 (TieA) interacts with cultured gastric epithelial cells and induces IL8 secretion via NF-κB mediated pathway.

Science.gov (United States)

Devi, Savita; Ansari, Suhail A; Vadivelu, Jamuna; Mégraud, Francis; Tenguria, Shivendra; Ahmed, Niyaz

2014-02-01

The envisaged roles and partly understood functional properties of Helicobacter pylori protein HP0986 are significant in the context of proinflammatory and or proapoptotic activities, the two important facilitators of pathogen survival and persistence. In addition, sequence analysis of this gene predicts a restriction endonuclease function which remained unknown thus far. To evaluate the role of HP0986 in gastric inflammation, we studied its expression profile using a large number of clinical isolates but a limited number of biopsies and patient sera. Also, we studied antigenic role of HP0986 in altering cytokine responses of human gastric epithelial (AGS) cells including its interaction with and localization within the AGS cells. For in vitro expression study of HP0986, 110 H. pylori clinical isolates were cultured from patients with functional dyspepsia. For expression analysis by qRT PCR of HP0986, 10 gastric biopsy specimens were studied. HP0986 was also used to detect antibodies in patient sera. AGS cells were incubated with recombinant HP0986 to determine cytokine response and NF-κB activation. Transient transfection with HP0986 cloned in pEGFPN1 was used to study its subcellular localization or homing in AGS cells. Out of 110 cultured H. pylori strains, 34 (31%) were positive for HP0986 and this observation was correlated with in vitro expression profiles. HP0986 mRNA was detected in 7 of the 10 biopsy specimens. Further, HP0986 induced IL-8 secretion in gastric epithelial cells in a dose and time-dependent manner via NF-κB pathway. Serum antibodies against HP0986 were positively associated with H. pylori positive patients. Transient transfection of AGS cells revealed both cytoplasmic and nuclear localization of HP0986. HP0986 was moderately prevalent in clinical isolates and its expression profile in cultures and gastric biopsies points to its being naturally expressed. Collective observations including the induction of IL-8 via TNFR1 and NF

HP fotovõistluse finalistid veebis / Henrik Roonemaa

Index Scriptorium Estoniae

Roonemaa, Henrik

2002-01-01

Hewlett-Packardi koduleheküljel on üleval rahvusvahelise digitaalfotograafia konkursi HP Invision Digital Photography Awards 2002 finalistide tööd, nende hulgas Katrin Arraku foto "Minu kala Vallo"

Effect of Mutations on HP Lattice Proteins

Science.gov (United States)

Shi, Guangjie; Vogel, Thomas; Landau, David; Li, Ying; Wüst, Thomas

2013-03-01

Using Wang-Landau sampling with approriate trial moves[2], we investigate the effect of different types of mutations on lattice proteins in the HP model. While exact studies have been carried out for short HP proteins[3], the systems we investigate are of much larger size and hence not accessible for exact enumerations. Based on the estimated density of states, we systematically analyse the changes in structure and degeneracy of ground states of particular proteins and measure thermodynamic quantities like the stability of ground states and the specific heat, for example. Both, neutral mutations, which do not change the structure and stability of ground states, as well as critical mutations, which do change the thermodynamic behavior qualitatively, have been observed. Research supported by NSF

Biosynthesis and characterization of diblock copolymer of p(3-hydroxypropionate)-block-p(4-hydroxybutyrate) from recombinant Escherichia coli

DEFF Research Database (Denmark)

Tripathi, Lakshmi; Wu, Linping; Meng, Dechuan

2013-01-01

Poly(4-hydroxybutyrate) (P4HB) is a highly elastic polymer, whereas poly(3-hydroxypropionate) (P3HP) is a polymer with enormous tensile strength. This study aimed to biosynthesize a block copolymer consisting of soft P4HB block with a strong P3HP block to gain unique and excellent material proper...

Analysis of the human HP1 interactome reveals novel binding partners

DEFF Research Database (Denmark)

Rosnoblet, Claire; Vandamme, Julien; Völkel, Pamela

2011-01-01

Heterochromatin protein 1 (HP1) has first been described in Drosophila as an essential component of constitutive heterochromatin required for stable epigenetic gene silencing. Less is known about the three mammalian HP1 isotypes CBX1, CBX3 and CBX5. Here, we applied a tandem affinity purification...

ComPAQS: a compact concentric UV/visible spectrometer, providing a new tool for air quality monitoring from space

Science.gov (United States)

Leigh, Roland J.; Whyte, C.; Cutter, M. A.; Lobb, D. R.; Monks, P. S.

2017-11-01

Under the first phase of the Centre for Earth Observation Instrumentation (CEOI), a breadboard demonstrator of a novel UV/VIS spectrometer has been developed. Using designs from Surrey Satellite Technology Ltd (SSTL) the demonstrator has been constructed and tested at the University of Leicester's Space Research Centre. This spectrometer provides an exceptionally compact instrument for differential optical absorption spectroscopy (DOAS) applications from LEO, GEO, HAP or ground-based platforms. Measurement of atmo spheric compounds with climate change or air quality implications is a key driver for the ground and space-based Earth Observation communities. Techniques using UV/VIS spectroscopy such as DOAS provide measurements of ozone profiles, aerosol optical depth, certain Volatile Organic Compounds, halogenated species, and key air quality parameters including tropospheric nitrogen dioxide. Compact instruments providing the necessary optical performance and spectral resolution are therefore a key enabling technology. The Compact Air Quality Spectrometer (CompAQS) features a concentric arrangement of a spherical meniscus lens, a concave spherical mirror and a suitable curved diffraction grating. This compact design provides efficiency and performance benefits over traditional concepts, improving the precision and spatial resolution available from space borne instruments with limited weight and size budgets. The breadboard spectrometer currently operating at the University of Leicester offers high throughput with a spectral range from 310 to 450 nm at 0.5nm(UV) to 1.0nm (visible) resolution, suitable for DOAS applications. The concentric design is capable of handling high relative apertures, owing to spherical aberration and coma being near zero at all surfaces. The design also provides correction for transverse chromatic aberration and distortion, in addition to correcting for the distortion called `smile' - the curvature of the slit image formed at each

The CompHP Core Competencies Framework for Health Promotion in Europe

Science.gov (United States)

Barry, Margaret M.; Battel-Kirk, Barbara; Dempsey, Colette

2012-01-01

Background: The CompHP Project on Developing Competencies and Professional Standards for Health Promotion in Europe was developed in response to the need for new and changing health promotion competencies to address health challenges. This article presents the process of developing the CompHP Core Competencies Framework for Health Promotion across…

Helicobacter pylori stool antigen test (HpSA) for the diagnosis of gastric infection

International Nuclear Information System (INIS)

Faruqui, A.N.; Majid, U.; Ahmed, L.; Khalil, M.; Hussan, M.U.

2007-01-01

To determine the accuracy of Helicobacter pylori Stool Antigen test (HpSA), compared with endoscopic histopathology for the diagnosis of gastric Helicobecter pylori infection. A total of 50 patients underwent endoscopy for gastric antral mucosal tissue biopsy for histopathology of H.pylori and advised for HpSA. Patient's information including age, gender, past history, presenting signs and symptoms, results of HpSA and histopathology were recorded. Sensitivity analysis was performed to calculate sensitivity, specificity, and predictive values of HpSA. Among 50 patients, 48% males and 52% females (M: F 1: 1.08), a total of 27 (54%) were true positive while 20 (40%) were true negative. Two patients were false negative and only one was false positive. Sensitivity of HpSA was, therefore, 93.1%, specificity 95% and positive and negative predictive values were 96.42% and 90.9% respectively. Helicobacter pylori stool antigen was an accurate and reliable test for the diagnosis of gastric H. pylori infection. (author)

Modular hp-FEM system HERMES and its application to Maxwell´s equations

Czech Academy of Sciences Publication Activity Database

Vejchodský, Tomáš; Šolín, P.; Zítka, M.

2007-01-01

Roč. 76, č. 2 (2007), s. 223-228 ISSN 0378-4754. [MODELLING 2005. Plzeň, 04.06.2005-08.06.2005] R&D Projects: GA ČR GP201/04/P021 Institutional research plan: CEZ:AV0Z10190503 Keywords : hp-FEM * time-harmonic Maxwell´s equations * hierarchic higher-order edge elements Subject RIV: BA - General Mathematics Impact factor: 0.738, year: 2007

HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.

Science.gov (United States)

Osbourn, Megan; Soares, Dinesh C; Vacca, Francesco; Cohen, E Suzanne; Scott, Ian C; Gregory, William F; Smyth, Danielle J; Toivakka, Matilda; Kemter, Andrea M; le Bihan, Thierry; Wear, Martin; Hoving, Dennis; Filbey, Kara J; Hewitson, James P; Henderson, Holly; Gonzàlez-Cìscar, Andrea; Errington, Claire; Vermeren, Sonja; Astier, Anne L; Wallace, William A; Schwarze, Jürgen; Ivens, Alasdair C; Maizels, Rick M; McSorley, Henry J

2017-10-17

Infection by helminth parasites is associated with amelioration of allergic reactivity, but mechanistic insights into this association are lacking. Products secreted by the mouse parasite Heligmosomoides polygyrus suppress type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. Here, we identified H. polygyrus Alarmin Release Inhibitor (HpARI), an IL-33-suppressive 26-kDa protein, containing three predicted complement control protein (CCP) modules. In vivo, recombinant HpARI abrogated IL-33, group 2 innate lymphoid cell (ILC2) and eosinophilic responses to Alternaria allergen administration, and diminished eosinophilic responses to Nippostrongylus brasiliensis, increasing parasite burden. HpARI bound directly to both mouse and human IL-33 (in the cytokine's activated state) and also to nuclear DNA via its N-terminal CCP module pair (CCP1/2), tethering active IL-33 within necrotic cells, preventing its release, and forestalling initiation of type 2 allergic responses. Thus, HpARI employs a novel molecular strategy to suppress type 2 immunity in both infection and allergy. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

Quantitative kinetics of In-111 autologous (In-AP) and homologous (Cr-HP) platelets in immune thrombocytopenic purpura (ITP)

International Nuclear Information System (INIS)

Lotter, M.G.; Heyns, A.D.P.; Badenhorst, P.N.; Minnaar, P.C.

1984-01-01

Contrary to the accepted view, the authors have found that platelet turnover is not always increased in ITP if the mean platelet survival time (PS) is measured with In-AP. The authors investigated the possible cause of the discrepancy by comparing kinetics of In-AP with those of Cr-HP in 10 patients with ITP. PS was estimated with the multiple hit model. The equilibrium and final in vivo distribution of In-AP was quantitated with the geometrical mean method. The patients could be divided into either those with splenic or diffuse RES platelet destruction. The authors conclude that in ITP platelet survival of In-AP is significantly (P < .05) longer than that of Cr-HP. Platelet turnover measured with In-AP is only normal in patients with mainly splenic platelet sequestration. Results with Cr-HP give a false impression of PS. It seems that in ITP those patients with severe disease also have a platelet production defect

General requirements to implement the personal dose equivalent Hp(10) in Brazil

International Nuclear Information System (INIS)

Lopes, Amanda Gomes; Silva, Francisco Cesar Augusto da

2017-01-01

To update the dosimetry quantity with the international community, Brazil is changing the Individual Dose Hx to the Personal Dose Equivalent Hp(10). A bibliographical survey on the technical and administrative requirements of nine countries that use Hp(10) was carried out to obtain the most relevant ones. All of them follow IEC and ISO guidelines for technical requirements, while administrative requirements change from country to country. Based on countries experiences, this paper presents a list of important general requirements to implement Hp(10) and to prepare the Brazilian requirements according to the international scientific community. (author)

General requirements to implement the personal dose equivalent Hp(10) in Brazil

Energy Technology Data Exchange (ETDEWEB)

Lopes, Amanda Gomes; Silva, Francisco Cesar Augusto da, E-mail: amandagl@bolsista.ird.gov.br [Instituto de Radioproteção e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

2017-07-01

To update the dosimetry quantity with the international community, Brazil is changing the Individual Dose Hx to the Personal Dose Equivalent Hp(10). A bibliographical survey on the technical and administrative requirements of nine countries that use Hp(10) was carried out to obtain the most relevant ones. All of them follow IEC and ISO guidelines for technical requirements, while administrative requirements change from country to country. Based on countries experiences, this paper presents a list of important general requirements to implement Hp(10) and to prepare the Brazilian requirements according to the international scientific community. (author)

Quasielastic 3Hp scattering at 2.5 GeV/c triton momentum

International Nuclear Information System (INIS)

Blinov, A.V.; Chuvilo, I.V.; Ergakov, V.A.

1982-01-01

The differential cross sections of the quasielastic 3 Hp-scattering at a 2.5 GeV/c tritium momentum (Tsub(p)=318 MeV) have been measured using the ITEP 80 cm hydrogen buble chamber. The experimental results are compared with the predictions of the Glauber-Sitenke multiple scattering theory combined with the the completeness condition for the excited nucleus wave functions. The validity of the Glauber sum rule for the differential cross sections is investigated

Peak {sup 14}CO{sub 2} excretion, symptoms and eradication of H.Pylori (HP) in patients with duodenal ulcer (DU)

Energy Technology Data Exchange (ETDEWEB)

Batholomeusz, F.D.L.; Bellon, M. [Royal Adelaide Hospital, Adelaide, SA (Australia). Department of Nuclear Medicine

1997-12-01

Full text: Many factors including drug efficacy, compliance and antibiotic resistance may influence HP eradication rates. The {sup 14}C urea breath test is a sensitive method of confirming HP colonisation and eradication. The aim was to study the relationships between the peak {sup 14}CO{sub 2} counts on the breath test prior to treatment, smoking and HP eradication, and to assess if persistent or recurrent symptoms implied failure of eradication. 117 patients with DU and HP colonisation proved on biopsy or rapid urease test who underwent {sup 14}C urea breath tests at least one month after eradication treatment was ceased were studied (69 male, 48 female, mean age 41, range 26-87 y). Questionnaire assessment of treatment regimen, smoking history and dyspeptic symptoms was made at the time of the breath test. A sub-group of 43 patients who had pre- and post-treatment breath tests was studied to assess if high peak {sup 14}CO{sub 2} counts on the pre-treatment breath test related to treatment success. The mean peak initial {sup 14}CO{sub 2} excretion in those who were treated successfully was 4187 (+ 472) counts/min and for failures 4817 (+739) counts/min (p 0.24). Dyspeptic symptoms were present in 36% of patients treated successfully and 37% where treatment failed. The eradication rate for the 56 smokers was 75 per cent, and the 61 non-smokers 83%. (p>0.2) High {sup 14}CO{sub 2} excretion on the initial {sup 14}C urea breath test and smoking are not associated with a significantly lower rate of HP eradication and the presence of dyspepsia is not a good indicator of persistent infection

Urinary 1-hydroxypyrene (1-HP) in environmental and occupational studies--a review

DEFF Research Database (Denmark)

Hansen, Ase M; Mathiesen, Line; Pedersen, Marie

2008-01-01

, volume (and time) or concentrations of creatinine are often used to standardize for diuresis. To be able to compare the included studies we recalculated the given concentrations to mumol 1-HP/mol creatinine. In conclusion, the highest concentrations of urinary 1-HP are found among workers...

hp-HGS strategy for inverse 3D DC resistivity logging measurement simulations

KAUST Repository

Gajda-Zaǵorska, Ewa; Paszý nski, Maciej; Schaefer, Robert; Pardo, David; Calo, Victor M.

2012-01-01

In this paper we present a twin adaptive strategy hp-HGS for solving inverse problems related to 3D DC borehole resistivity measurement simulations. The term “simulation of measurements” is widely used by the geophysical community. A quantity of interest, voltage, is measured at a receiver electrode located in the logging instrument. We use the self-adaptive goal-oriented hp-Finite Element Method (hp-FEM) computer simulations of the process of measurements in deviated wells (when the angle between the borehole and formation layers are < 90 deg). We also employ the hierarchical genetic search (HGS) algorithm to solve the inverse problem. Each individual in the population represents a single configuration of the formation layers. The evaluation of the individual is performed by solving the direct problem by means of the hp-FEM algorithm and by comparison with measured logging curve. We conclude the paper with some discussion on the parallelization of the algorithm.

hp-HGS strategy for inverse 3D DC resistivity logging measurement simulations

KAUST Repository

Gajda-Zaǵorska, Ewa

2012-06-02

In this paper we present a twin adaptive strategy hp-HGS for solving inverse problems related to 3D DC borehole resistivity measurement simulations. The term “simulation of measurements” is widely used by the geophysical community. A quantity of interest, voltage, is measured at a receiver electrode located in the logging instrument. We use the self-adaptive goal-oriented hp-Finite Element Method (hp-FEM) computer simulations of the process of measurements in deviated wells (when the angle between the borehole and formation layers are < 90 deg). We also employ the hierarchical genetic search (HGS) algorithm to solve the inverse problem. Each individual in the population represents a single configuration of the formation layers. The evaluation of the individual is performed by solving the direct problem by means of the hp-FEM algorithm and by comparison with measured logging curve. We conclude the paper with some discussion on the parallelization of the algorithm.

Synthesis gas generation by high pressure partial oxidation (HP POX {sup registered}); Synthesegaserzeugung durch Hochdruck-Partialoxidation (HP POX {sup registered})

Energy Technology Data Exchange (ETDEWEB)

Meyer, B.; Seifert, P.; Zeissler, R. [Technische Univ. Bergakademie Freiberg (Germany). Inst. fuer Energieverfahrenstechnik und Chemieingenieurwesen; Walter, St. [Lurgi AG, Frankfurt/Main (Germany)

2005-05-01

The high pressure synthesis gas process HP POX (High Pressure Partial Oxidation) is a thermal conversion process, which converts e.g. natural gas or heavy residue oil with oxygen to fuel or synthesis gas. Innovative burner technologies allow autothermal catalytic or non-catalytic reforming of gaseous feedstock and gasification of liquid feed streams. Hydrogen and carbon monoxide rich synthesis gases with low methane content (particularly suitable as feed gas for methanol synthesis) can be generated. The technology represents a new generation of entrained flow gasification characterized by the new developed equipment and a design pressure of 100 bar. According to the feeds, the HP POX gasification process is the link between the downstream synthesis chemistry (use of gaseous and liquid feeds) and the power plant process (liquid or solid fuels as suspension or slurry). The HP POX development is carried out together with the Lurgi AG at the Institute for Energy Process Engineering and Chemical Engineering (IEC). The 5 MW HP POX test plant built in Freiberg by Lurgi is core of the joint research project, which is supported by the German Federal Ministry of Economics and Labour (BMWA), the Saxon Ministry of Science and the Fine Arts (SMWK), and the mg technologies ag (parent company of Lurgi). The conducted sets of experiments indicate that the unit can be operated in a safe, smooth and stable manner. During this periods of operation, which included nine test campaigns for autothermal reforming (ATR), the maximum pressure for ATR amounted to 70 bar which exceeds the current benchmark in industry for 30 bar. Main objective of R and D work is the development of modelling tools for extreme gasification conditions and different gasification principles of up to 100 bar. These tools are supposed to improve the understanding of the entire gasification process. Their development requires a systematic investigation of the reaction mechanisms and the interactions with the process

Cross-Validation of the Spanish HP-Version of the Jefferson Scale of Empathy Confirmed with Some Cross-Cultural Differences.

Science.gov (United States)

Alcorta-Garza, Adelina; San-Martín, Montserrat; Delgado-Bolton, Roberto; Soler-González, Jorge; Roig, Helena; Vivanco, Luis

2016-01-01

Medical educators agree that empathy is essential for physicians' professionalism. The Health Professional Version of the Jefferson Scale of Empathy (JSE-HP) was developed in response to a need for a psychometrically sound instrument to measure empathy in the context of patient care. Although extensive support for its validity and reliability is available, the authors recognize the necessity to examine psychometrics of the JSE-HP in different socio-cultural contexts to assure the psychometric soundness of this instrument. The first aim of this study was to confirm its psychometric properties in the cross-cultural context of Spain and Latin American countries. The second aim was to measure the influence of social and cultural factors on the development of medical empathy in health practitioners. The original English version of the JSE-HP was translated into International Spanish using back-translation procedures. The Spanish version of the JSE-HP was administered to 896 physicians from Spain and 13 Latin American countries. Data were subjected to exploratory factor analysis using principal component analysis (PCA) with oblique rotation (promax) to allow for correlation among the resulting factors, followed by a second analysis, using confirmatory factor analysis (CFA). Two theoretical models, one based on the English JSE-HP and another on the first Spanish student version of the JSE (JSE-S), were tested. Demographic variables were compared using group comparisons. A total of 715 (80%) surveys were returned fully completed. Cronbach's alpha coefficient of the JSE for the entire sample was 0.84. The psychometric properties of the Spanish JSE-HP matched those of the original English JSE-HP. However, the Spanish JSE-S model proved more appropriate than the original English model for the sample in this study. Group comparisons among physicians classified by gender, medical specialties, cultural and cross-cultural backgrounds yielded statistically significant differences

Procedure Development to Determine Organic Compounds in the PM{sub 1}-2.5 and PM{sub 2}.5-10 Fractions of Atmospheric Aerosols; Desarrollo de la Metodologia para la Determinacion de los Compuestos Organicos en las Fracciones PM{sub 1}-2.5 y PM{sub 2}.5-10 del Aerosol Atmosferico

Energy Technology Data Exchange (ETDEWEB)

Pindado, O; Perez, R M

2011-07-28

An analytical procedure development to measure organic compounds such as aliphatic hydrocarbons, polycyclic aromatic compounds, n-alcohols and fatty acids in PM{sub 1}-2.5 and PM{sub 2}.5-10 of atmospheric aerosol is accomplished. The development encompasses an optimization of extraction step, derivatization step and chromatographic analysis. The method developed consists in a microwave extraction, followed by a stage of fractionation and analysis by GC/MS. (Author) 20 refs.

Clerget 100 hp heavy-oil engine

Science.gov (United States)

Leglise, Pierre

1931-01-01

A complete technical description of the Clerget heavy-oil engine is presented along with the general characteristics. The general characteristics are: 9 cylinders, bore 120 mm, stroke 130 mm, four-stroke cycle engine, rated power limited to 100 hp at 1800 rpm; weight 228 kg; propeller with direct drive and air cooling. Moving parts, engine block, and lubrication are all presented.

MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation

Science.gov (United States)

Agarwal, Noopur; Hardt, Tanja; Brero, Alessandro; Nowak, Danny; Rothbauer, Ulrich; Becker, Annette; Leonhardt, Heinrich; Cardoso, M. Cristina

2007-01-01

There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1γ relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation. PMID:17698499

The Grasso 5HP as a heat pump compressor for a dairy industry; De Grasso 5HP als warmtepompcompressor voor melkfabriek

Energy Technology Data Exchange (ETDEWEB)

Vermeer, H.; Gerritsen, J.; Unsworth, R. [Gea Grenco, Den Bosch (Netherlands)

2011-12-15

GEA Refrigeration has supplied a new cooling system with an additional stage heat pump to the milk factory Robert Wiseman for the pasteurisation of milk. The required condensation temperature of the additional stage heat pump is around 80C. The Grasso 5HP reciprocating compressor has a favorable energy consumption and can be used for this application. The limitation of this compressor, however, sets requirements to the design of the installation. [Dutch] Gea Refrigeration heeft een nieuwe koelinstallatie met extratrapwarmtepomp geleverd aan zuivelbedrijf Robert Wiseman ten behoeve van pasteuriseren van melk. De hiervoor benodigde condensatietemperatuur van de extratrapwarmtepomp bedraagt ongeveer 80C. De Grasso 5HP hogedrukzuigercompressor heeft een gunstig energiegebruik en kan voor deze toepassing ingezet worden. De begrenzing van het toepassingsgebied van deze compressor stelt echter eisen aan het ontwerp van de installatie.

Discrete Maximum Principle for a 1D Problem with Piecewise-Constant Coefficients Solved by hp-FEM

Czech Academy of Sciences Publication Activity Database

Vejchodský, Tomáš; Šolín, Pavel

2007-01-01

Roč. 15, č. 3 (2007), s. 233-243 ISSN 1570-2820 R&D Projects: GA ČR GP201/04/P021; GA ČR GA102/05/0629 Institutional research plan: CEZ:AV0Z10190503; CEZ:AV0Z20570509 Keywords : discrete maximum principle * hp-FEM * Poisson equation Subject RIV: BA - General Mathematics

Cross-validation of the Spanish HP-Version of the Jefferson Scale of Empathy confirmed with some cross-cultural differences

Directory of Open Access Journals (Sweden)

Adelina Alcorta-Garza

2016-07-01

Full Text Available Context: Medical educators agree that empathy is essential for physicians’ professionalism. The Health Professional Version of the Jefferson Scale of Empathy (JSE-HP was developed in response to a need for a psychometrically sound instrument to measure empathy in the context of patient care. Although extensive support for its validity and reliability is available, the authors recognize the necessity to examine psychometrics of the JSE-HP in different socio-cultural contexts to assure the psychometric soundness of this instrument. The first aim of this study was to confirm its psychometric properties in the cross-cultural context of Spain and Latin American countries. The second aim was to measure the influence of social and cultural factors on the development of medical empathy in health practitioners.Methods: The original English version of the JSE-HP was translated into International Spanish using back-translation procedures. The Spanish version of the JSE-HP was administered to 896 physicians from Spain and thirteen Latin American countries. Data were subjected to exploratory factor analysis using principal component analysis with oblique rotation (promax to allow for correlation among the resulting factors, followed by a second analysis, using confirmatory factor analysis. Two theoretical models, one based on the English JSE-HP and another on the first Spanish student version of the JSE (JSE-S, were tested. Demographic variables were compared using group comparisons.Results: A total of 715 (80% surveys were returned fully completed. Cronbach’s alpha coefficient of the JSE for the entire sample was 0.84. The psychometric properties of the Spanish JSE-HP matched those of the original English JSE-HP. However, the Spanish JSE-S model proved more appropriate than the original English model for the sample in this study. Group comparisons among physicians classified by gender, medical specialties, cultural and cross-cultural backgrounds yielded

New approach to calculate the true-coincidence effect of HpGe detector

Energy Technology Data Exchange (ETDEWEB)

Alnour, I. A., E-mail: aaibrahim3@live.utm.my, E-mail: ibrahim.elnour@yahoo.com [Department of Physics, Faculty of Pure and Applied Science, International University of Africa, 12223 Khartoum (Sudan); Wagiran, H. [Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai,Johor (Malaysia); Ibrahim, N. [Faculty of Defence Science and Technology, National Defence University of Malaysia, Kem Sungai Besi, 57000 Kuala Lumpur (Malaysia); Hamzah, S.; Elias, M. S. [Malaysia Nuclear Agency (MNA), Bangi, 43000 Kajang, Selangor D.E. (Malaysia); Siong, W. B. [Chemistry Department, Faculty of Resource Science & Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak (Malaysia)

2016-01-22

The corrections for true-coincidence effects in HpGe detector are important, especially at low source-to-detector distances. This work established an approach to calculate the true-coincidence effects experimentally for HpGe detectors of type Canberra GC3018 and Ortec GEM25-76-XLB-C, which are in operation at neutron activation analysis lab in Malaysian Nuclear Agency (NM). The correction for true-coincidence effects was performed close to detector at distances 2 and 5 cm using {sup 57}Co, {sup 60}Co, {sup 133}Ba and {sup 137}Cs as standard point sources. The correction factors were ranged between 0.93-1.10 at 2 cm and 0.97-1.00 at 5 cm for Canberra HpGe detector; whereas for Ortec HpGe detector ranged between 0.92-1.13 and 0.95-100 at 2 and 5 cm respectively. The change in efficiency calibration curve of the detector at 2 and 5 cm after correction was found to be less than 1%. Moreover, the polynomial parameters functions were simulated through a computer program, MATLAB in order to find an accurate fit to the experimental data points.

The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori

LENUS (Irish Health Repository)

Douillard, Francois P

2010-04-08

Abstract Background Helicobacter pylori is the causative agent for gastritis, and peptic and duodenal ulcers. The bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. The biochemistry and genetics of flagellar biogenesis in H. pylori has not been fully elucidated. Bioinformatics analysis suggested that the gene HP0256, annotated as hypothetical, was a FliJ homologue. In Salmonella, FliJ is a chaperone escort protein for FlgN and FliT, two proteins that themselves display chaperone activity for components of the hook, the rod and the filament. Results Ablation of the HP0256 gene in H. pylori significantly reduced motility. However, flagellin and hook protein synthesis was not affected in the HP0256 mutant. Transmission electron transmission microscopy revealed that the HP0256 mutant cells displayed a normal flagellum configuration, suggesting that HP0256 was not essential for assembly and polar localisation of the flagella in the cell. Interestingly, whole genome microarrays of an HP0256 mutant revealed transcriptional changes in a number of genes associated with the flagellar regulon and the cell envelope, such as outer membrane proteins and adhesins. Consistent with the array data, lack of the HP0256 gene significantly reduced adhesion and the inflammatory response in host cells. Conclusions We conclude that HP0256 is not a functional counterpart of FliJ in H. pylori. However, it is required for full motility and it is involved, possibly indirectly, in expression of outer membrane proteins and adhesins involved in pathogenesis and adhesion.

Study of the kinematically complete breakup reaction 2H(p vector, pp)n at Ep=13.0 MeV with polarized protons

International Nuclear Information System (INIS)

Rauprich, G.; Lemaitre, S.; Niessen, P.; Nyga, K.R.; Reckenfelderbaeumer, R.; Sydow, L.; Paetz genannt Schieck, H.; Witala, H.; Gloeckle, W.

1991-01-01

The breakup reaction 2 H(p vector,pp)n at E p =13.0 MeV has been measured in a kinematically complete experiment with polarized protons in four special kinematical situations: np final-state interaction, pp quasi-free scattering, collinearity and symmetric space star. These configurations are identical to those of a corresponding 2 H(n,nn) 1 H experiment. The cross sections d 3 σ/dΩ 3 dΩ 4 dS and the vector analyzing powers A y are presented as a function of the arclength of the relevant kinematical loci. They are compared to the rigorous Faddeev calculations using realistic meson-exchange NN potentials, i.e. the Paris and Bonn potential. Comparison with the cross section data of the analog neutron experiment is made. In the quasi-free scattering and the space-star cross sections marked discrepancies with the theoretical predictions, in the latter case also with the neutron results are found. In the collinear and final-state interaction situations the agreement is rather good as well as for all analyzing powers and is even improved by taking the finite angular spread into account. (orig.)

DAMT - DISTRIBUTED APPLICATION MONITOR TOOL (HP9000 VERSION)

Science.gov (United States)

Keith, B.

1994-01-01

processes. The independent processes communicate with each other via UNIX sockets through a Virtual Path router, or Switcher. The Switcher maintains a routing table showing the host of each component process of the tool, eliminating the need for each process to do so. The Central Monitor Complex provides the single application program interface (API) to the user and coordinates the activities of DAMT. The Central Monitor Complex is itself divided into independent objects that perform its functions. The component objects are the Central Monitor, the Process Locator, the Circuit Locator, and the Traffic Reporter. Each of these objects is an independent, asynchronously executing process. User requests to the tool are interpreted by the Central Monitor. The Process Locator identifies whether a named process is running on a monitored host and which host that is. The circuit between any two processes in the distributed application is identified using the Circuit Locator. The Traffic Reporter handles communication with the LAN Analyzer and accumulates traffic updates until it must send a traffic report to the user. The Remote Monitor process is replicated on each monitored host. It serves the Central Monitor Complex processes with application process information. The Remote Monitor process provides access to operating systems information about currently executing processes. It allows the Process Locator to find processes and the Circuit Locator to identify circuits between processes. It also provides lifetime information about currently monitored processes. The LAN Analyzer consists of two processes. Low-level monitoring is handled by the Sniffer. The Sniffer analyzes the raw data on a single, physical LAN. It responds to commands from the Analyzer process, which maintains the interface to the Traffic Reporter and keeps track of which circuits to monitor. DAMT is written in C-language for HP-9000 series computers running HP-UX and Sun 3 and 4 series computers running SunOS. DAMT

Structure of trihydrated rare-earth acid diphosphates LnHP2O7·3H2O (Ln=La, Er)

International Nuclear Information System (INIS)

Ben Moussa, S.; Ventemillas, S.; Cabeza, A.; Gutierrez-Puebla, E.; Sanz, J.

2004-01-01

In trihydrated lanthanum acid-diphosphates LnHP 2 O 7 ·3H 2 O, prepared from acid LnCl 3 and Na 4 P 2 O 7 solutions (pH=1), two crystal forms were obtained. Layered structures of two representative members of this family have been determined by single-crystal X-ray diffraction (XRD) technique. In the case of orthorhombic LaHP 2 O 7 ·3H 2 O (type I), lanthanum cations are ninefold coordinated and diphosphate groups adopt a staggered (alternated) configuration. In the case of triclinic ErHP 2 O 7 ·3H 2 O (type II), erbium cations are eightfold coordinated and diphosphate groups adopt an eclipsed configuration. In agreement with Infrared (IR) spectroscopic data, a bended configuration for diphosphate groups has been deduced. In both structures, one-dimensional chains of edge-sharing rare-earth polyhedra are linked together by diphosphate groups to form the phosphate layers. In both diphosphates, PO 4 and HPO 4 environments have been identified by 31 P MAS-NMR technique. In the two compounds, OH groups of HPO 4 tetrahedra point out of diphosphate planes interacting with adjacent layers. In La-diphosphate, the interaction between HPO 4 groups and water molecules of adjacent layers is favored; however, in Er-diphosphate, the interaction between phosphate acid groups of contiguous layers is produced. Based on structural information deduced, differences detected in IR and NMR spectra of two disphosphates are discussed

Goal-Oriented Self-Adaptive hp Finite Element Simulation of 3D DC Borehole Resistivity Simulations

KAUST Repository

Calo, Victor M.; Pardo, David; Paszyński, Maciej R.

2011-01-01

(adjusting polynomial orders of approximation) or hp (both) refinements on the finite elements. The new parallel implementation utilizes a computational mesh shared between multiple processors. All computational algorithms, including automatic hp goal

A Chaotic Oscillator Based on HP Memristor Model

Directory of Open Access Journals (Sweden)

Guangyi Wang

2015-01-01

Full Text Available This paper proposes a simple autonomous memristor-based oscillator for generating periodic signals. Applying an external sinusoidal excitation to the autonomous system, a nonautonomous oscillator is obtained, which contains HP memristor model and four linear circuit elements. This memristor-based oscillator can generate periodic, chaotic, and hyperchaotic signals under the periodic excitation and an appropriate set of circuit parameters. It also shows that the system exhibits alternately a hidden attractor with no equilibrium and a self-excited attractor with a line equilibrium as time goes on. Furthermore, some specialties including burst chaos, irregular periodic bifurcations, and nonintermittence chaos of the circuit are found by theoretical analysis and numerical simulations. Finally, a discrete model for the HP memristor is given and the main statistical properties of this memristor-based oscillator are verified via DSP chip experiments and NIST (National Institute of Standards and Technology tests.

A sensitization effect of hematoporphyrin oligomer (HpO) and caffeine for X-ray radiation of skin cancer

International Nuclear Information System (INIS)

Ishiguro, Kazumori; Ueda, Keiichi; Miyoshi, Norio; Nakanishi, Kazuo; Fukuda, Masaru

1990-01-01

Human malignant melanoma cells (G-361) were irradiated with X-ray for different irradiation times. The amount of DNA damage was much increased by the treatment of the cells with hematoporphyrin oligomer (HpO) before X-ray irradiation. Furthermore, it was found that the caffeine inhibited effectively the repair of the damaged DNA. Furthermore, HpO was administered before X-ray irradiation of transplantable skin tumor in C3H mice and/or caffeine was to be given the mouse after X-ray irradiation. The growth of the irradiated tumor combined with HpO and/or caffeine was more inhibited, and the necrotic area was more expanded than that of the tumor irradiated with X-ray only. Labeling Index utilizing bromodeoxyuridine (BrdU) in the viable area of the treated tumor decreased when HpO and/or caffeine were administered. The amount of DNA damage more increased in the irradiated tumor cells given HpO than the only irradiated cells. And, the repair of the damaged DNA was more delayed in the irradiated tumor cells combined with HpO and caffeine than those with other treatments. In conclusion, it was considered that HpO might be a radioactive sensitizer and caffeine enhanced the inhibition of the repair of DNA damaged. We hope that the treatment of X-ray radiation combined with HpO and caffeine is useful for clinical treatment of human malignant tumor. (author) 54 refs

A sensitization effect of hematoporphyrin oligomer (HpO) and caffeine for X-ray radiation of skin cancer

Energy Technology Data Exchange (ETDEWEB)

Ishiguro, Kazumori; Ueda, Keiichi; Miyoshi, Norio; Nakanishi, Kazuo; Fukuda, Masaru (Fukui Medical School, Matsuoka (Japan))

1990-05-01

Human malignant melanoma cells (G-361) were irradiated with X-ray for different irradiation times. The amount of DNA damage was much increased by the treatment of the cells with hematoporphyrin oligomer (HpO) before X-ray irradiation. Furthermore, it was found that the caffeine inhibited effectively the repair of the damaged DNA. Furthermore, HpO was administered before X-ray irradiation of transplantable skin tumor in C3H mice and/or caffeine was to be given the mouse after X-ray irradiation. The growth of the irradiated tumor combined with HpO and/or caffeine was more inhibited, and the necrotic area was more expanded than that of the tumor irradiated with X-ray only. Labeling Index utilizing bromodeoxyuridine (BrdU) in the viable area of the treated tumor decreased when HpO and/or caffeine were administered. The amount of DNA damage more increased in the irradiated tumor cells given HpO than the only irradiated cells. And, the repair of the damaged DNA was more delayed in the irradiated tumor cells combined with HpO and caffeine than those with other treatments. In conclusion, it was considered that HpO might be a radioactive sensitizer and caffeine enhanced the inhibition of the repair of DNA damaged. We hope that the treatment of X-ray radiation combined with HpO and caffeine is useful for clinical treatment of human malignant tumor. (author) 54 refs.

Monte Carlo determination of the conversion coefficients Hp(3)/Ka in a right cylinder phantom with 'PENELOPE' code. Comparison with 'MCNP' simulations.

Science.gov (United States)

Daures, J; Gouriou, J; Bordy, J M

2011-03-01

This work has been performed within the frame of the European Union ORAMED project (Optimisation of RAdiation protection for MEDical staff). The main goal of the project is to improve standards of protection for medical staff for procedures resulting in potentially high exposures and to develop methodologies for better assessing and for reducing, exposures to medical staff. The Work Package WP2 is involved in the development of practical eye-lens dosimetry in interventional radiology. This study is complementary of the part of the ENEA report concerning the calculations with the MCNP-4C code of the conversion factors related to the operational quantity H(p)(3). In this study, a set of energy- and angular-dependent conversion coefficients (H(p)(3)/K(a)), in the newly proposed square cylindrical phantom made of ICRU tissue, have been calculated with the Monte-Carlo code PENELOPE and MCNP5. The H(p)(3) values have been determined in terms of absorbed dose, according to the definition of this quantity, and also with the kerma approximation as formerly reported in ICRU reports. At a low-photon energy (up to 1 MeV), the two results obtained with the two methods are consistent. Nevertheless, large differences are showed at a higher energy. This is mainly due to the lack of electronic equilibrium, especially for small angle incidences. The values of the conversion coefficients obtained with the MCNP-4C code published by ENEA quite agree with the kerma approximation calculations obtained with PENELOPE. We also performed the same calculations with the code MCNP5 with two types of tallies: F6 for kerma approximation and *F8 for estimating the absorbed dose that is, as known, due to secondary electrons. PENELOPE and MCNP5 results agree for the kerma approximation and for the absorbed dose calculation of H(p)(3) and prove that, for photon energies larger than 1 MeV, the transport of the secondary electrons has to be taken into account.

Characteristics of particle-bound polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in atmosphere used in carbon black feeding process at a tire manufacturing plant.

Science.gov (United States)

Chuang, Kuen-Yuan; Lai, Chia-Hsiang; Peng, Yen-Ping; Yen, Ting-Yu

2015-12-01

Concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) were estimated for different particle size distributions in a carbon black feeding process at a tire manufacturing plant on 15 days in March and April of 2014. A total of 75 integrated air samples were collected using a micro-orifice uniform deposition impactor (MOUDI). Particle-bound PCDD/Fs were analyzed using a high-resolution gas chromatograph/high-resolution mass spectrometer (HRGC/HRMS). Concentrations of thoracic particles and total particles produced in the carbon black feeding process of a tire manufacturing plant were measured in ranges of 0.19-2.61 and 0.28-4.22 mg/m(3), respectively. On all sampling days, the three most abundant species of PCDD/Fs were OCDD, 1,2,3,4,6,7,8-HpCDF, and OCDF. The mean concentrations of total PCDD/Fs were 0.74-6.83 pg/m(3) within five particle size ranges. Total I-TEQ in particulate matter (PM)18 and PM2.5-10, respectively. However, the total I-TEQ of thoracic PM contributed approximately 74 % of the total I-TEQ of total PM. The assessment of health risk indicates that exposure to fractions of thoracic PM by inhalation poses a significant cancer risk (>10(-6)).

Design and realisation of a microprogramme for the analysis of nuclear spectroscopic data on the intelligent terminal, H.P.2648A

International Nuclear Information System (INIS)

Tendeku, F.K.

1980-01-01

A microprogramme has been developed for the analysis of nuclear spectroscopic data on the microprocessor-controlled terminal, H.P. 2648A. The terminal enables data to be processed locally. Many operations normally requiring connection to a computer can be performed on a stand alone basis. The principal aspects of the microprogramme are the graphical display of spectral data, the automatic and manual extraction of peaks, and the determination of peak characteristics. Among the features of the microprogramme are algorithms which enable a rapid graphical display of data in logarithmic and linear scales and in divers modes as well as functions enabling image amplification. The automatic peak searching algorithm represents a modified form of the Mariscotti method. The peak analysis procedure offers two options. The first makes use of the data directly. The second is based on the determination of the parameters of the gaussian function which best fits the data points, using the non-linear least squares principle. The terminal has been successfully interfaced to an IBM 370 computer through a satellite computer using the Parallel Duplex Register module of Hewlett Packard [fr

Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box

International Nuclear Information System (INIS)

Moriyama, Tetsuji; Sangel, Percival; Yamaguchi, Hiroki; Obuse, Chikashi; Miyamoto, Yoichi; Oka, Masahiro; Yoneda, Yoshihiro

2015-01-01

Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4). In vitro transport assay demonstrated that nuclear localization of GFP-TRIM28 NLS is mediated by importin αs, in conjunction with importin β1 and Ran. Further, we demonstrated that HP1 and importin αs compete for binding to TRIM28. Together, our findings suggest that importin α has an essential role in the nuclear delivery and preferential HP1 interaction of TRIM28. - Highlights: • TRIM28 contains an NLS within the 462-494 amino acid region. • The nuclear import of TRIM28 is mediated by importin α/importin β1. • TRIM28 NLS overlaps with HP1 Box. • HP1 and importin α compete for binding to TRIM28

Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box

Energy Technology Data Exchange (ETDEWEB)

Moriyama, Tetsuji; Sangel, Percival [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Yamaguchi, Hiroki [School of Medicine, Osaka University, Osaka 565-0871 (Japan); Obuse, Chikashi [Graduate School of Life Science, Hokkaido University, Sapporo 001-0021 (Japan); Miyamoto, Yoichi [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Oka, Masahiro, E-mail: moka@nibiohn.go.jp [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); Yoneda, Yoshihiro, E-mail: y-yoneda@nibiohn.go.jp [National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan)

2015-07-03

Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4). In vitro transport assay demonstrated that nuclear localization of GFP-TRIM28 NLS is mediated by importin αs, in conjunction with importin β1 and Ran. Further, we demonstrated that HP1 and importin αs compete for binding to TRIM28. Together, our findings suggest that importin α has an essential role in the nuclear delivery and preferential HP1 interaction of TRIM28. - Highlights: • TRIM28 contains an NLS within the 462-494 amino acid region. • The nuclear import of TRIM28 is mediated by importin α/importin β1. • TRIM28 NLS overlaps with HP1 Box. • HP1 and importin α compete for binding to TRIM28.

78 FR 48468 - Hewlett Packard Company, Hewlett Packard Enterprise Business Unit, EG HP Storage, Enterprise...

Science.gov (United States)

2013-08-08

..., Hewlett Packard Enterprise Business Unit, EG HP Storage, Enterprise Storage, Servers and Networking Storage, APP Management, Research and Development Group, Andover, Massachusetts; Notice of Investigation... Enterprise Business Unit, EG HP Storage, Enterprise Storage, Servers and Networking Storage Division, APP...

dAdd1 and dXNP prevent genome instability by maintaining HP1a localization at Drosophila telomeres.

Science.gov (United States)

Chavez, Joselyn; Murillo-Maldonado, Juan Manuel; Bahena, Vanessa; Cruz, Ana Karina; Castañeda-Sortibrán, América; Rodriguez-Arnaiz, Rosario; Zurita, Mario; Valadez-Graham, Viviana

2017-12-01

Telomeres are important contributors to genome stability, as they prevent linear chromosome end degradation and contribute to the avoidance of telomeric fusions. An important component of the telomeres is the heterochromatin protein 1a (HP1a). Mutations in Su(var)205, the gene encoding HP1a in Drosophila, result in telomeric fusions, retrotransposon regulation loss and larger telomeres, leading to chromosome instability. Previously, it was found that several proteins physically interact with HP1a, including dXNP and dAdd1 (orthologues to the mammalian ATRX gene). In this study, we found that mutations in the genes encoding the dXNP and dAdd1 proteins affect chromosome stability, causing chromosomal aberrations, including telomeric defects, similar to those observed in Su(var)205 mutants. In somatic cells, we observed that dXNP and dAdd1 participate in the silencing of the telomeric HTT array of retrotransposons, preventing anomalous retrotransposon transcription and integration. Furthermore, the lack of dAdd1 results in the loss of HP1a from the telomeric regions without affecting other chromosomal HP1a binding sites; mutations in dxnp also affected HP1a localization but not at all telomeres, suggesting a specialized role for dAdd1 and dXNP proteins in locating HP1a at the tips of the chromosomes. These results place dAdd1 as an essential regulator of HP1a localization and function in the telomere heterochromatic domain.

A replica exchange Monte Carlo algorithm for protein folding in the HP model

Directory of Open Access Journals (Sweden)

Shmygelska Alena

2007-09-01

Full Text Available Abstract Background The ab initio protein folding problem consists of predicting protein tertiary structure from a given amino acid sequence by minimizing an energy function; it is one of the most important and challenging problems in biochemistry, molecular biology and biophysics. The ab initio protein folding problem is computationally challenging and has been shown to be NP MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaacqWFneVtcqqGqbauaaa@3961@-hard even when conformations are restricted to a lattice. In this work, we implement and evaluate the replica exchange Monte Carlo (REMC method, which has already been applied very successfully to more complex protein models and other optimization problems with complex energy landscapes, in combination with the highly effective pull move neighbourhood in two widely studied Hydrophobic Polar (HP lattice models. Results We demonstrate that REMC is highly effective for solving instances of the square (2D and cubic (3D HP protein folding problem. When using the pull move neighbourhood, REMC outperforms current state-of-the-art algorithms for most benchmark instances. Additionally, we show that this new algorithm provides a larger ensemble of ground-state structures than the existing state-of-the-art methods. Furthermore, it scales well with sequence length, and it finds significantly better conformations on long biological sequences and sequences with a provably unique ground-state structure, which is believed to be a characteristic of real proteins. We also present evidence that our REMC algorithm can fold sequences which exhibit significant interaction between termini in the hydrophobic core relatively easily. Conclusion We demonstrate that REMC utilizing the pull move

Genome-wide analysis reveals the extent of EAV-HP integration in domestic chicken.

Science.gov (United States)

Wragg, David; Mason, Andrew S; Yu, Le; Kuo, Richard; Lawal, Raman A; Desta, Takele Taye; Mwacharo, Joram M; Cho, Chang-Yeon; Kemp, Steve; Burt, David W; Hanotte, Olivier

2015-10-14

EAV-HP is an ancient retrovirus pre-dating Gallus speciation, which continues to circulate in modern chicken populations, and led to the emergence of avian leukosis virus subgroup J causing significant economic losses to the poultry industry. We mapped EAV-HP integration sites in Ethiopian village chickens, a Silkie, Taiwan Country chicken, red junglefowl Gallus gallus and several inbred experimental lines using whole-genome sequence data. An average of 75.22 ± 9.52 integration sites per bird were identified, which collectively group into 279 intervals of which 5 % are common to 90 % of the genomes analysed and are suggestive of pre-domestication integration events. More than a third of intervals are specific to individual genomes, supporting active circulation of EAV-HP in modern chickens. Interval density is correlated with chromosome length (P < 2.31(-6)), and 27 % of intervals are located within 5 kb of a transcript. Functional annotation clustering of genes reveals enrichment for immune-related functions (P < 0.05). Our results illustrate a non-random distribution of EAV-HP in the genome, emphasising the importance it may have played in the adaptation of the species, and provide a platform from which to extend investigations on the co-evolutionary significance of endogenous retroviral genera with their hosts.

Weighted curve-fitting program for the HP 67/97 calculator

International Nuclear Information System (INIS)

Stockli, M.P.

1983-01-01

The HP 67/97 calculator provides in its standard equipment a curve-fit program for linear, logarithmic, exponential and power functions that is quite useful and popular. However, in more sophisticated applications, proper weights for data are often essential. For this purpose a program package was created which is very similar to the standard curve-fit program but which includes the weights of the data for proper statistical analysis. This allows accurate calculation of the uncertainties of the fitted curve parameters as well as the uncertainties of interpolations or extrapolations, or optionally the uncertainties can be normalized with chi-square. The program is very versatile and allows one to perform quite difficult data analysis in a convenient way with the pocket calculator HP 67/97

HpBase: A genome database of a sea urchin, Hemicentrotus pulcherrimus.

Science.gov (United States)

Kinjo, Sonoko; Kiyomoto, Masato; Yamamoto, Takashi; Ikeo, Kazuho; Yaguchi, Shunsuke

2018-04-01

To understand the mystery of life, it is important to accumulate genomic information for various organisms because the whole genome encodes the commands for all the genes. Since the genome of Strongylocentrotus purpratus was sequenced in 2006 as the first sequenced genome in echinoderms, the genomic resources of other North American sea urchins have gradually been accumulated, but no sea urchin genomes are available in other areas, where many scientists have used the local species and reported important results. In this manuscript, we report a draft genome of the sea urchin Hemincentrotus pulcherrimus because this species has a long history as the target of developmental and cell biology in East Asia. The genome of H. pulcherrimus was assembled into 16,251 scaffold sequences with an N50 length of 143 kbp, and approximately 25,000 genes were identified in the genome. The size of the genome and the sequencing coverage were estimated to be approximately 800 Mbp and 100×, respectively. To provide these data and information of annotation, we constructed a database, HpBase (http://cell-innovation.nig.ac.jp/Hpul/). In HpBase, gene searches, genome browsing, and blast searches are available. In addition, HpBase includes the "recipes" for experiments from each lab using H. pulcherrimus. These recipes will continue to be updated according to the circumstances of individual scientists and can be powerful tools for experimental biologists and for the community. HpBase is a suitable dataset for evolutionary, developmental, and cell biologists to compare H. pulcherrimus genomic information with that of other species and to isolate gene information. © 2018 Japanese Society of Developmental Biologists.

175Hp contrarotating homopolar motor design report

Science.gov (United States)

Cannell, Michael J.; Drake, John L.; McConnell, Richard A.; Martino, William R.

1994-06-01

A normally conducting contrarotating homopolar motor has been designed and constructed. The reaction torque, in the outer rotor, from the inner rotor is utilized to produce true contrarotation. The machine utilizes liquid cooled conductors, high performance liquid metal current collectors, and ferrous conductors in the active region. The basic machine output is 175 hp at + or - 1,200 rpm with an input of 4 volts and 35,000 amps.

Discontinuous Galerkin discretization and hp-refinement for the resolution of the neutron transport equation

International Nuclear Information System (INIS)

Fournier, Damien; Le-Tellier, Romain; Herbin, Raphaele

2013-01-01

This paper presents an hp-refinement method for a first order scalar transport reaction equation discretized by a discontinuous Galerkin method. First, the theoretical rates of convergence of h- and p-refinement are recalled and numerically tested. Then, in order to design some meshes, we propose two different estimators of the local error on the spatial domain. These quantities are analyzed and compared depending on the regularity of the solution so as to find the best way to lead the refinement process and the best strategy to choose between h- and p-refinement. Finally, the different possible refinement strategies are compared first on analytical examples and then on realistic applications for neutron transport in a nuclear reactor core. (authors)

HP-67 calculator programs for thermodynamic data and phase diagram calculations

International Nuclear Information System (INIS)

Brewer, L.

1978-01-01

This report is a supplement to a tabulation of the thermodynamic and phase data for the 100 binary systems of Mo with the elements from H to Lr. The calculations of thermodynamic data and phase equilibria were carried out from 5000 0 K to low temperatures. This report presents the methods of calculation used. The thermodynamics involved is rather straightforward and the reader is referred to any advanced thermodynamic text. The calculations were largely carried out using an HP-65 programmable calculator. In this report, those programs are reformulated for use with the HP-67 calculator; great reduction in the number of programs required to carry out the calculation results

2H(p, pp) n reaction as a probe of the short-range nuclear force

International Nuclear Information System (INIS)

Haftel, M.I.; Petersen, E.L.; Wallace, J.M.

1976-01-01

We examine the feasibility of using the 2 H(p, pp) n reaction as a means of extracting information about the short-range behavior of the nuclear force not obtainable from N-N scattering experiments. To do this we use several separable potentials and examine the predicted cross section in various regions of phase space and for beam energies between 14 and 65 MeV. The questions that we address are likely to be insensitive to Coulomb effects. Both the form factor and the energy dependence of the potentials have been modified from the usual Yamaguchi form. The form of the energy dependence is chosen to obtain phase-shift equivalence for two different form factors while guaranteeing a unitary two-body scattering amplitude. The sensitivity of breakup results to the on-shell and off-shell aspects of the nuclear force is examined and discussed. Significant on-shell sensitivity occurs for breakup amplitudes in all states and for cross sections over all regions of phase space. Off-shell sensitivity appears only in the S = 1/2, L = 0 breakup amplitudes, with all S = 3/2 and all L > 0 amplitudes exhibiting negligible off-shell dependence. This result leads to only a very small (< or = 5%) off-shell sensitivity for quasifree scattering. However, cross sections far from quasifree scattering, and in particular cross sections in the final-state interaction region of phase space, exhibit as much as a 50% variation for phase-shift-equivalent potentials. This sensitivity is small at low beam energy and increases with increasing energy. The energy dependence at negative energies of one potential is also altered to adjust the triton binding energy. This enables us to compare phase-shift-equivalent potentials differing off shell but predicting the same triton binding energy. The energy dependence of this potential is somewhat unconventional. Fixing of the triton binding energy reduces the off-shell sensitivity appreciably only for E approximately-less-than 20 MeV

A study on directional resistivity logging-while-drilling based on self-adaptive hp-FEM

Science.gov (United States)

Liu, Dejun; Li, Hui; Zhang, Yingying; Zhu, Gengxue; Ai, Qinghui

2014-12-01

Numerical simulation of resistivity logging-while-drilling (LWD) tool response provides guidance for designing novel logging instruments and interpreting real-time logging data. In this paper, based on self-adaptive hp-finite element method (hp-FEM) algorithm, we analyze LWD tool response against model parameters and briefly illustrate geosteering capabilities of directional resistivity LWD. Numerical simulation results indicate that the change of source spacing is of obvious influence on the investigation depth and detecting precision of resistivity LWD tool; the change of frequency can improve the resolution of low-resistivity formation and high-resistivity formation. The simulation results also indicate that the self-adaptive hp-FEM algorithm has good convergence speed and calculation accuracy to guide the geologic steering drilling and it is suitable to simulate the response of resistivity LWD tools.

Susceptibility of the tomato mutant high pigment-2dg (hp-2dg) to Orobanche spp. infection.

Science.gov (United States)

López-Ráez, Juan Antonio; Charnikhova, Tatsiana; Mulder, Patrick; Kohlen, Wouter; Bino, Raoul; Levin, Ilan; Bouwmeester, Harro

2008-08-13

The consumption of natural products with potential health benefits has been continuously growing, and enhanced pigmentation is of major economic importance in fruits and vegetables. The tomato hp-2 ( dg ) is an important mutant line that has been introgressed into commercial tomato cultivars marketed as lycopene rich tomatoes (LRT) because of their enhanced fruit pigmentation, attributed to higher levels of carotenoids, including lycopene. Strigolactones are signaling compounds that mediate host finding in root parasitic plants and are biosynthetically derived from carotenoids. Considering the high carotenoid content of the hp-2 ( dg ) mutant, we studied its susceptibility to the root parasite Orobanche. In a field experiment, the average number of Orobanche aegyptiaca plants growing on hp-2 ( dg ) was surprisingly significantly reduced compared with its isogenic wild-type counterpart. In vitro assays and LC-MS/MS analysis showed that this reduction was associated with a lower production of strigolactones, which apparently renders the high-carotenoid hp-2 ( dg ) mutant less susceptible to Orobanche.

Effects of recombinant plasmid pEgr-p53 transfected stably in combination with X-irradiation on cell cycle progression and proliferation in human SKOV-3 tumor cells in vitro

International Nuclear Information System (INIS)

Dong Lihua; Liu Feng; Li Yanbo; Fu Shibo; Gong Shouliang

2008-01-01

Objective: To investigate the effect of recombinant plasmid pEgr-hp53 transfected stably in combination with X-ray irradiation on the cell cycle progression and the proliferation in human SKOV-3 tumor cells. Methods: pEgr-hp53 and pcDNA3.1 packaged with liposome were stably transfected into SKOV-3 cells in vitro. SKOV-3-hp53 and SKOV-3-vect were irradiated with 0, 0.5, 2.0 and 5.0 Gy X-rays, respectively, i.e. 8 experimental groups. The SKOV-3 cell proliferation and the cell cycle progression were measured with flow cytometry and cell growth curve, respectively. Results: Compared with 0 Gy group, the cell counts in SKOV-3- hp53 plus different doses of irradiation groups 2 d after irradiation decreased significantly (P 0 /G 1 cells increased significantly (P 2 /M cells decreased in varying degrees. The cell counts in SKOV-3-hp53 plus irradiation group were significantly lower than those in corresponding SKOV-3-vect plus irradiation group, the cell counts 4-8 d after irradiation with 0.5 Gy, 2 d after 2.0 Gy irradiation and 6 d after 5.0 Gy irradiation decreased significantly (P 0 /G 1 cells increased significantly (P 2 /M cells decreased significantly (P 1 arrest in SKOV-3 cells and inhibits the cell proliferation. Ionizing radiation can activate early growth response-1 (Egr-1) gene promoter and increase the expression of p53 gene, and enhance the inhibition of tumor cell growth. (authors)

Asymmetry in the momentum distribution of H+p from dissociative ionization of H2 controlled by the carrier-envelope phase of a few-cycle pulse

Science.gov (United States)

Zeng, Shuo; Anis, Fatima; Esry, B. D.

2010-03-01

We present theoretical results on the interaction of H2 with an ultrashort linearly polarized laser pulse of 6 fs duration. We investigate the carrier-envelope phase effects of this ultrashort laser pulse on the asymmetry of the momentum distribution of H+p along the laser polarization direction. To do so, we model the H2 ionization by launching a coherent wavepacket on H2^+ potential curves at each field maximum, and then propagate wavepackets in time on H2^+ Born-Oppenheimer potential curves coupled by the laser. Nuclear rotation and vibration are both included in the Schrödinger equation for H2^+ . Our results will be compared to a recent experimental measurement [1]. We will also compare our results to calculations neglecting rotation and discuss the limitations of such a model.[4pt] [1] Manuel Kremer et al., Phys. Rev. Lett. 103.213003 (2009)

Majority- and minority-carrier deep level traps in proton-irradiated n+/p-InGaP space solar cells

International Nuclear Information System (INIS)

Dharmarasu, Nethaji; Yamaguchi, Masafumi; Bourgoin, Jacques C.; Takamoto, Tatsuya; Ohshima, Takeshi; Itoh, Hisayoshi; Imaizumi, Mitsuru; Matsuda, Sumio

2002-01-01

We report the properties of observed defects in n + /p-InGaP solar cells created by irradiation of protons of different energies. Three majority (hole) and a minority-carrier traps, labeled respectively as HP1 (E v +0.90±0.05 eV), HP2 (E v +0.73±0.05 eV), H2 (E v +0.55 eV), and EP1 (E c -0.54 eV), were identified using deep level transient spectroscopy. All majority-carrier traps were found to act as recombination centers. While the H2 trap present in the proton-irradiated p-InGaP was found to anneal out by minority-carrier injection, the other traps were not

KDM2A integrates DNA and histone modification signals through a CXXC/PHD module and direct interaction with HP1

Science.gov (United States)

Borgel, Julie; Tyl, Marek; Schiller, Karin; Pusztai, Zsofia; Dooley, Christopher M.; Deng, Wen; Wooding, Carol; White, Richard J.; Warnecke, Tobias; Leonhardt, Heinrich; Busch-Nentwich, Elisabeth M.

2017-01-01

Abstract Functional genomic elements are marked by characteristic DNA and histone modification signatures. How combinatorial chromatin modification states are recognized by epigenetic reader proteins and how this is linked to their biological function is largely unknown. Here we provide a detailed molecular analysis of chromatin recognition by the lysine demethylase KDM2A. Using biochemical approaches we identify a nucleosome interaction module within KDM2A consisting of a CXXC type zinc finger, a PHD domain and a newly identified Heterochromatin Protein 1 (HP1) interaction motif that mediates direct binding between KDM2A and HP1. This nucleosome interaction module enables KDM2A to decode nucleosomal H3K9me3 modification in addition to CpG methylation signals. The multivalent engagement with DNA and HP1 results in a nucleosome binding circuit in which KDM2A can be recruited to H3K9me3-modified chromatin through HP1, and HP1 can be recruited to unmodified chromatin by KDM2A. A KDM2A mutant deficient in HP1-binding is inactive in an in vivo overexpression assay in zebrafish embryos demonstrating that the HP1 interaction is essential for KDM2A function. Our results reveal a complex regulation of chromatin binding for both KDM2A and HP1 that is modulated by DNA- and H3K9-methylation, and suggest a direct role for KDM2A in chromatin silencing. PMID:28180290

Negative-tone imaging with EUV exposure for 14nm hp and beyond

Science.gov (United States)

Tsubaki, Hideaki; Nihashi, Wataru; Tsuchihashi, Toru; Fujimori, Toru; Momota, Makoto; Goto, Takahiro

2015-03-01

Manipulation of dissolution properties by changing organic solvent developer and rinse material provides a novel technology to obtain fine pattern beyond the limitation of imaging system based on alkaline developer. QCM study showed no swelling character in negative-tone imaging (NTI) process even for current developer of n-butyl acetate (nBA). Actually, NTI process has shown advantages on resolution and line-width roughness (LWR) in loose pitch around 30 ~ 45 nm hp as a consequence of its non-swelling character. On the other hand, bridge and collapse limited its resolution below 20 nm hp, indicating that non-negligible amount of swelling still exists for tight pitch resolution. We investigated effects of solubility parameter of organic solvents on resolution below 20 nm hp. A bridge was reduced with a decrease in the solubility parameter dp from nBA. On the other hand, much lower dp caused film remaining due to its extremely slow Rmax. Based on these results, we newly developed FN-DP301 containing organic solvent with smaller dp than nBA. Although rinse solvent gave negligible effects on bridge, there is a clear improvement on pattern collapse only in case of using new rinse solvent of FN-RP311. Lithographic performances of NTI process using nBA and FN-DP301 together with the other organic solvents were described in this paper under exposures with an E-beam and a EUV light. It is emphasized that 14 nm hp resolution was obtained only using FN-DP301 as a developer and FN-RP311 as a rinse under E-beam exposure. NTI showed 43% faster photospeed in comparison with PTI at 16 nm hp, indicating that NTI is applicable to obtain high throughput with maintaining resolution. In addition, sub-20 nm trench was obtained using NTI without bridge under EUV exposure, all of which are attributed to the low swelling character of NTI process. Similarly, NTI was able to print 20 nm dots using NXE:3100 with only a little peeling. Conversely CH patterning was significantly worse with NTI

Human factors involvement in bringing the power of AI to a heterogeneous user population

Science.gov (United States)

Czerwinski, Mary; Nguyen, Trung

1994-01-01

The Human Factors involvement in developing COMPAQ QuickSolve, an electronic problem-solving and information system for Compaq's line of networked printers, is described. Empowering customers with expert system technology so they could solve advanced networked printer problems on their own was a major goal in designing this system. This process would minimize customer down-time, reduce the number of phone calls to the Compaq Customer Support Center, improve customer satisfaction, and, most importantly, differentiate Compaq printers in the marketplace by providing the best, and most technologically advanced, customer support. This represents a re-engineering of Compaq's customer support strategy and implementation. In its first generation system, SMART, the objective was to provide expert knowledge to Compaq's help desk operation to more quickly and correctly answer customer questions and problems. QuickSolve is a second generation system in that customer support is put directly in the hands of the consumers. As a result, the design of QuickSolve presented a number of challenging issues. Because the produce would be used by a diverse and heterogeneous set of users, a significant amount of human factors research and analysis was required while designing and implementing the system. Research that shaped the organization and design of the expert system component as well.

Determination of effective dose in anisotropic gamma radiation fields: application of dosimeters calibrated in terms of Hp(10)

International Nuclear Information System (INIS)

Chumak, V. V.; Bakhanova, E. V.

2003-01-01

In this presentation authors deals with determination of effective dose in anisotropic gamma radiation fields. It was conclude that: - Straightforward application of Hp(10) as surrogate for E may not work under certain conditions; - Partial data on behavior of E and Hp(10) for different dosimeters allow to estimate E/Hp(10) conversion coefficients for any particular composite source; - In practical situations, anisotropy of workplace fields may be measured by six- collimator device assessing contribution to a dose from six orthogonal directions; - Reasonably conservative conversion coefficients may be assessed for given energy spectrum and degree of anisotropy of workplace fields; - For strongly anisotropic fields multiple dosimetry approach gives the best estimate of E comparing to plain Hp(10) readouts or integral conversion coefficients

On a Weak Discrete Maximum Principle for hp-FEM

Czech Academy of Sciences Publication Activity Database

Šolín, Pavel; Vejchodský, Tomáš

-, č. 209 (2007), s. 54-65 ISSN 0377-0427 R&D Projects: GA ČR(CZ) GA102/05/0629 Institutional research plan: CEZ:AV0Z20570509; CEZ:AV0Z10190503 Keywords : discrete maximum principle * hp-FEM Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.943, year: 2007

PPADS and suramin as antagonists at cloned P2Y- and P2U-purinoceptors.

Science.gov (United States)

Charlton, S J; Brown, C A; Weisman, G A; Turner, J T; Erb, L; Boarder, M R

1996-06-01

1. The effect of suramin and pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS) on the stimulation of phospholipase C in 1321N1 cells transfected with the human P2U-purinoceptor (h-P2U-1321N1 cells) or with the turkey P2Y-purinoceptor (t-P2Y-1321N1 cells) was investigated. 2-Methylthioadenosine triphosphate (2MeSATP) was used as the agonist at t-P2Y-1321N1 cells and uridine triphosphate (UTP) at h-P2U-1321N1 cells. 2. Suramin caused a parallel shift to the right of the concentration-response curves for 2MeSATP in the t-P2Y-1321N1 cells, yielding a Schild plot with a slope of 1.16 +/- 0.08 and a pA2 value of 5.77 +/- 0.11. 3. Suramin also caused a shift to the right of concentration-response curves for UTP in the h-P2U-1321N1 cells, and on Schild plots gave a slope different from unity (1.57 +/- 0.19) and an apparent pA2 value of 4.32 +/- 0.13. Suramin was therefore a less potent antagonist at the P2U-purinoceptor than the P2Y-purinoceptor. 4. In the presence of the ectonucleotidase inhibitor, ARL 67156 (6-N,N-diethyl-beta,gamma-dibromomethylene-D-ATP) there was no significant difference in the EC50 or shapes of curves with either cell type, and no difference in pA2 values for suramin. 5. PPADS caused an increase in the EC50 for 2MeSATP in the t-P2Y-1321N1 cells. The Schild plot had a slope different from unity (0.55 +/- 0.15) and an X-intercept corresponding to an apparent pA2 of 5.98 +/- 0.65. 6. PPADS up to 30 microM had no effect on the concentration-response curve for UTP with the h-P2U-1321N1 cells. 7. In conclusion, suramin and PPADS show clear differences in their action at the 2 receptor types, in each case being substantially more effective as an antagonist at the P2Y-purinoceptor than at the P2U-purinoceptor. Ectonucleotidase breakdown had little influence on the nature of the responses at the two receptor types, or in their differential sensitivity to suramin.

Evolution of the Eurasian Ice Sheets during the Last Deglaciation (25-10 kyr)

Science.gov (United States)

Hughes, A. L. C.; Gyllencreutz, R.; Mangerud, J.; Svendsen, J. I.; Lohne, Ø. S.

2014-12-01

Both the timing of maximum extent and subsequent pace of retreat of the interconnected Eurasian (British-Irish, Scandinavian, Svalbard-Barents-Kara Sea) Ice Sheets were spatially variable likely reflecting contrasts in response to forcing mechanisms, geographical settings and glacial dynamics both between individual ice sheets and ice-sheet sectors. For example the maximum limit along the western continental shelf edge was reached up to 3,000 years earlier than the maximum, mainly terrestrial, limits in the east. We present new time-slice reconstructions of the ice-sheet evolution through the last deglaciation based on a compiled chronology of over 5,000 dates and published ice-margin positions. Ice-sheet margins are depicted every 1,000 years (25-10 kyr) and include uncertainty estimates (represented by maximum, minimum and most-credible lines). The new ice-sheet scale reconstructions summarise and provide the means for direct comparison of the empirical geological record against simulations of the deglacial ice-sheet evolution from numerical and isostatic ice-sheet modelling and the timing of abrupt events observed in deglacial climate and ocean records. The reconstruction process has identified both instances of conflicting evidence and gaps in the geological record that should be a focus for future studies. This work is part of an on-going project to reconstruct the changing limits of the Eurasian Ice Sheets through the last glacial cycle (www.uib.no/project/dated).

DhHP-6 extends lifespan of Caenorhabditis elegans by enhancing nuclear translocation and transcriptional activity of DAF-16.

Science.gov (United States)

Huang, Lei; Li, Pengfei; Wang, Guan; Guan, Shuwen; Sun, Xiaoli; Wang, Liping

2013-04-01

Earlier studies have demonstrated that Deuterohaemin-AlaHisThrValGluLys (DhHP-6), a novel porphyrin-peptide, increases lifespan and enhances stress resistance of Caenorhabditis elegans. To explore the possible mechanisms, in this study we investigated the roles of SIR-2.1 and DAF-16 in DhHP-6's function using wild-type and various other mutant strains of C. elegans. DhHP-6's effect was dependent upon DAF-16, and it did not extend the lifespan of the loss-of-function daf-16 mutant strain (daf-16(mu86) I). DhHP-6 enhanced DAF-16 translocation from cytoplasm to nuclei; and it increased DAF-16's transcriptional activity, likely by activating the SIR-2.1/DAF-16 complex. DhHP-6's effect was also dependent upon SIR-2.1, and it did not increase the lifespan of the worms with SIR-2.1 deacetylase activity inhibited by niacin amide (SIR-2.1 inhibitor) and SIR-2.1 RNA interference (RNAi). Niacin amide and RNAi increased DAF-16's nuclear localization; but they decreased DAF-16's transcriptional activity, likely by preventing the formation of the SIR-2.1/DAF-16 complex. These results suggest that DhHP-6 extends the lifespan of C. elegans via SIR 2.1 and DAF-16, and they provide new insights into the molecular mechanisms of aging.

Corifollitropin alfa compared to daily rFSH or HP-HMG in GnRH antagonist controlled ovarian stimulation protocol for patients undergoing assisted reproduction.

Science.gov (United States)

Souza, Priscila Morais Galvão; Carvalho, Bruno Ramalho de; Nakagawa, Hitomi Miura; Rassi, Thalita Reis Esselin; Barbosa, Antônio César Paes; Silva, Adelino Amaral

2017-06-01

This study aimed to compare the outcomes of controlled ovarian stimulation (COS) with corifollitropin alfa versus daily recombinant follicle-stimulating hormone (rRFSH) or highly purified human menopausal gonadotropin (HP-HMG) in patients undergoing in vitro fertilization (IVF) cycles based on gonadotropin-releasing hormone (GnRH) antagonist protocols. The primary endpoints were total number of oocytes and mature oocytes. This retrospective study looked into 132 controlled ovarian stimulation cycles from IVF or oocyte cryopreservation performed in a private human reproduction center between January 1 and December 31, 2014. Enrollment criteria: women aged 0.05). There were no significant differences in fertilization (76.9% vs. 76.8%, p=1.0), biochemical pregnancy (66.7% vs. 47.2%, p=0.1561) or embryo implantation rates (68.7% vs. 50%, p=0.2588) between the groups using corifollitropin alfa and rFSH or HMG, respectively. Corifollitropin alfa seems to be as effective as rFSH or HP-HMG when used in the first seven days of ovulation induction for patients undergoing assisted reproduction in GnRH antagonist protocols.

Stability of plasmas obtained in E.P.P.E; Stabilite des plasmas obtenus dans E. P. P. E

Energy Technology Data Exchange (ETDEWEB)

Adam, J; Rebut, P H; Torossian, A [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

1964-07-01

The stability results obtained on Mest have been extended on a machine ten times more powerful: EppE. A plasma stable during its whole confinement time has been observed. This confinement time seems to be limited by the resistive diffusion of the magnetic fields through the plasma. The main characteristics of this plasma are: Time of confinement: 200 {mu}s; T{sub e} {approx} T{sub i}: 40 eV; {beta} = 8*{pi}*k*T*n{sub e}/B{sup 2}: 8%; n{sub e}: 2.5*10{sup 15}/cm{sup 3}. (authors) [French] Les resultats sur la stabilite obtenus dans Mest ont ete developpes dans une machine dix fois plus puissante: EppE. Un plasma stable pendant toute la duree de son confinement a ete observe et la limitation de ce temps est bien expliquee par la diffusion resistive des champs magnetiques a travers le plasma. Les principales caracteristiques du plasma d'EppE sont: Temps de confinement: 200 {mu}s; T{sub e} {approx} T{sub i}: 40 eV; {beta} 8*{pi}*k*T*n{sub e}/B{sup 2}: 8%; n{sub e}: 2.5*10{sup 15}/cm{sup 3}.

A phantom for assessing the personal dose equivalent, HP(10)

International Nuclear Information System (INIS)

Santoro, C.; Filho, J.A

2013-01-01

Characteristics of a phantom designed to evaluate the personal dose equivalent, H P (10), and appropriate for photographic dosimetry are presented. It is called HP(10) phantom due to cavities constructed to insert dosimetric films at a depth of 10 mm. The H P (10) phantom is irradiated with ionizing radiation energy, E, from 45 to 1250 keV, with doses ranging from 0.2 to 50 mSv. It is positioned in the direction α = 0 °, and the radiation field focusing perpendicular to its front surface. So, are established calibration curves of dosimeters in the position conventionally true and quantities H P (10). It made a comparison between the responses obtained with the H P (10) phantom and responses obtained when using the calibration procedure recommended by ISO dosimeters. The ISO recommends getting the air kerma, Ka, for photons at test point of the radiation field by an ionization chamber. And through conversion coefficients, h pK (10; E, α), becomes the air kerma for H P (10). The ISO 4037-3 recommendation has been studied by researchers to ensure that the low energy spectral differences occur in radiation fields which are generated by various X-ray equipment, and induce changes in the percentages of conversion coefficients on the order of 10% to 90% . On the basis of the recommendations ISO, this article develops phantom able to assess the dose to the influence of scattering and absorption of radiation, its implications with respect to dosimetry, providing improvement in the assessment of doses. (author)

hp-FEM electromechanical transduction model of ionic polymer metal composites

Czech Academy of Sciences Publication Activity Database

Pugal, D.; Šolín, Pavel; Kim, K.; Aabloo, A.

2014-01-01

Roč. 260, April (2014), s. 135-148 ISSN 0377-0427 R&D Projects: GA ČR(CZ) GAP102/11/0498 Institutional support: RVO:61388998 Keywords : hp-FEM * Nernst-Planck * Poisson Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.266, year: 2014

Effect of antiparkinson drug HP-200 (Mucuna pruriens) on the central monoaminergic neurotransmitters.

Science.gov (United States)

Manyam, Bala V; Dhanasekaran, Muralikrishnan; Hare, Theodore A

2004-02-01

HP-200, which contains Mucuna pruriens endocarp, has been shown to be effective in the treatment of Parkinson's disease. Mucuna pruriens endocarp has also been shown to be more effective compared to synthetic levodopa in an animal model of Parkinson's disease. The present study was designed to elucidate the long-term effect of Mucuna pruriens endocarp in HP-200 on monoaminergic neurotransmitters and its metabolite in various regions of the rat brain. HP-200 at a dose of 2.5, 5.0 or 10.0 g/kg/day was mixed with rat chow and fed daily ad lib to Sprague-Dawley rats (n = 6 for each group) for 52 weeks. Controls (n = 6) received no drug. Random assignment was made for doses and control. The rats were sacrificed at the end of 52 weeks and the neurotransmitters were analyzed in the cortex, hippocampus, substantia nigra and striatum. Oral administration of Mucuna pruriens endocarp in the form of HP-200 had a significant effect on dopamine content in the cortex with no significant effect on levodopa, norepinephrine or dopamine, serotonin, and their metabolites- HVA, DOPAC and 5-HIAA in the nigrostriatal tract. The failure of Mucuna pruriens endocarp to significantly affect dopamine metabolism in the striatonigral tract along with its ability to improve Parkinsonian symptoms in the 6-hydorxydopamine animal model and humans may suggest that its antiparkinson effect may be due to components other than levodopa or that it has an levodopa enhancing effect. Copyright 2004 John Wiley & Sons, Ltd. Copyright 2004 John Wiley & Sons, Ltd.

High pressure effect on the color of minced cured restructured ham at different levels of drying, pH, and NaCl.

Science.gov (United States)

Bak, Kathrine Holmgaard; Lindahl, Gunilla; Karlsson, Anders H; Lloret, Elsa; Ferrini, Gabriele; Arnau, Jacint; Orlien, Vibeke

2012-03-01

Color changes of minced cured restructured ham was studied considering the effects of high pressure (HP) treatment (600MPa, 13°C, 5min), raw meat pH(24) (low, normal, high), salt content (15, 30g/kg), and drying (20%, 50% weight loss). Raw hams were selected based on pH(24) in Semimembranosus, mixed with additives, frozen, sliced, and dried using the Quick-Dry-Slice® process. Meat color (CIE 1976 L*a*b*) and reflectance spectra were measured before and after HP treatment. HP significantly increased L*, decreased a*, and decreased b* for restructured ham dried to 20% weight loss, regardless of salt content and pH(24). L* and a* were best preserved in high pH/high salt restructured ham. HP had no effect on the color of restructured ham dried to 50% weight loss. HP had no effect on the shape of reflectance curves, indicating that the pigment responsible for minced cured restructured ham color did not change due to HP. Copyright © 2011 Elsevier Ltd. All rights reserved.

Heterochromatin protein 1 (HP1a positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila.

Directory of Open Access Journals (Sweden)

Lucia Piacentini

2009-10-01

Full Text Available Heterochromatin Protein 1 (HP1a is a well-known conserved protein involved in heterochromatin formation and gene silencing in different species including humans. A general model has been proposed for heterochromatin formation and epigenetic gene silencing in different species that implies an essential role for HP1a. According to the model, histone methyltransferase enzymes (HMTases methylate the histone H3 at lysine 9 (H3K9me, creating selective binding sites for itself and the chromodomain of HP1a. This complex is thought to form a higher order chromatin state that represses gene activity. It has also been found that HP1a plays a role in telomere capping. Surprisingly, recent studies have shown that HP1a is present at many euchromatic sites along polytene chromosomes of Drosophila melanogaster, including the developmental and heat-shock-induced puffs, and that this protein can be removed from these sites by in vivo RNase treatment, thus suggesting an association of HP1a with the transcripts of many active genes. To test this suggestion, we performed an extensive screening by RIP-chip assay (RNA-immunoprecipitation on microarrays, and we found that HP1a is associated with transcripts of more than one hundred euchromatic genes. An expression analysis in HP1a mutants shows that HP1a is required for positive regulation of these genes. Cytogenetic and molecular assays show that HP1a also interacts with the well known proteins DDP1, HRB87F, and PEP, which belong to different classes of heterogeneous nuclear ribonucleoproteins (hnRNPs involved in RNA processing. Surprisingly, we found that all these hnRNP proteins also bind heterochromatin and are dominant suppressors of position effect variegation. Together, our data show novel and unexpected functions for HP1a and hnRNPs proteins. All these proteins are in fact involved both in RNA transcript processing and in heterochromatin formation. This suggests that, in general, similar epigenetic mechanisms

Space-time adaptive hp-FEM for problems with traveling sharp fronts

Czech Academy of Sciences Publication Activity Database

Šolín, Pavel; Korous, L.

2013-01-01

Roč. 95, č. 1 (2013), S709-S722 ISSN 0010-485X Institutional support: RVO:61388998 Keywords : adaptive hp-FEM * transient problems * sharp fronts Subject RIV: BA - General Mathematics Impact factor: 1.055, year: 2013

Helicobacter pylori HP1034 (ylxH) is required for motility

NARCIS (Netherlands)

van Amsterdam, Karin; van der Ende, Arie

2004-01-01

Background. Helicobacter pylori motility is essential for the colonization and persistence in the human gastric mucosa. So far, more than 50 genes have been described to play a role in flagellar biosynthesis. H. pylori YlxH (HP1034) is annotated as an ATP-binding protein. However, H. pylori YlxH

Two-Photon Activation of p-Hydroxyphenacyl Phototriggers: Toward Spatially Controlled Release of Diethyl Phosphate and ATP.

Science.gov (United States)

Houk, Amanda L; Givens, Richard S; Elles, Christopher G

2016-03-31

Two-photon activation of the p-hydroxyphenacyl (pHP) photoactivated protecting group is demonstrated for the first time using visible light at 550 nm from a pulsed laser. Broadband two-photon absorption measurements reveal a strong two-photon transition (>10 GM) near 4.5 eV that closely resembles the lowest-energy band at the same total excitation energy in the one-photon absorption spectrum of the pHP chromophore. The polarization dependence of the two-photon absorption band is consistent with excitation to the same S3 ((1)ππ*) excited state for both one- and two-photon activation. Monitoring the progress of the uncaging reaction under nonresonant excitation at 550 nm confirms a quadratic intensity dependence and that two-photon activation of the uncaging reaction is possible using visible light in the range 500-620 nm. Deprotonation of the pHP chromophore under mildly basic conditions shifts the absorption band to lower energy (3.8 eV) in both the one- and two-photon absorption spectra, suggesting that two-photon activation of the pHP chromophore may be possible using light in the range 550-720 nm. The results of these measurements open the possibility of spatially and temporally selective release of biologically active compounds from the pHP protecting group using visible light from a pulsed laser.

p53 expresado en la mucosa gástrica de pacientes infectados por Helicobacter pylori

Directory of Open Access Journals (Sweden)

G.A. Morales-Fuentes

2013-01-01

Conclusiones: Se demuestra asociación significativa entre Hp y la expresión de p53, incluso en pacientes con lesiones preneoplásicas y ya sin la presencia del Hp. Ya que la identificación de lesiones preneoplásicas es importante para la prevención del cáncer; la toma de biopsias rutinarias durante la endoscopia para la detección de Hp podría verse beneficiada de la inmunohistoquímica, identificando a pacientes con expresión de p53, un importante oncogén regulador.

The next step in coastal numerical models: spectral/hp element methods?

DEFF Research Database (Denmark)

Eskilsson, Claes; Engsig-Karup, Allan Peter; Sherwin, Spencer J.

2005-01-01

In this paper we outline the application of spectral/hp element methods for modelling nonlinear and dispersive waves. We present one- and two-dimensional test cases for the shallow water equations and Boussinesqtype equations – including highly dispersive Boussinesq-type equations....

Mechanisms of continental subduction and exhumation of HP and UHP rocks

NARCIS (Netherlands)

Burov, Evgene; Francois, Thomas; Yamato, Philippe; Wolf, Sylvie

We discuss possible scenarios of continental collision, and their relation to mechanisms of exhumation of HP and UHP rocks, inferred from thermo-mechanical numerical models accounting for thermo-rheological complexity of the continental lithosphere. Due to this complexity, mechanisms of continental

Influence of niobium addition on the high temperature mechanical properties of a centrifugally cast HP alloy

International Nuclear Information System (INIS)

Andrade, A.R.; Bolfarini, C.; Ferreira, L.A.M.; Vilar, A.A.A.; Souza Filho, C.D.; Bonazzi, L.H.C.

2015-01-01

The influence of niobium addition on the mechanical properties at high temperature of HP alloy has been investigated. Two HP alloys were centrifugally cast with a similar chemical composition differing only in the niobium content. Low strain rate high temperature tensile tests and creep-rupture tests were performed in the range of 900–1100 °C, and the results compared between the alloys. According to the results, the high temperature mechanical behavior of both alloys is controlled by several factors like solid solution, network of eutectic carbides, intradendritic precipitation and dendrite spacing. A significant increase in the mechanical properties for the HP alloy with niobium addition was found within the temperature range of 900–1050 °C. Beyond this temperature the mechanical behavior of both alloys is basically the same

Switching the JLab Accelerator Operations Environment from an HP-UX Unix-based to a PC/Linux-based environment

International Nuclear Information System (INIS)

Mcguckin, Theodore

2008-01-01

The Jefferson Lab Accelerator Controls Environment (ACE) was predominantly based on the HP-UX Unix platform from 1987 through the summer of 2004. During this period the Accelerator Machine Control Center (MCC) underwent a major renovation which included introducing Redhat Enterprise Linux machines, first as specialized process servers and then gradually as general login servers. As computer programs and scripts required to run the accelerator were modified, and inherent problems with the HP-UX platform compounded, more development tools became available for use with Linux and the MCC began to be converted over. In May 2008 the last HP-UX Unix login machine was removed from the MCC, leaving only a few Unix-based remote-login servers still available. This presentation will explore the process of converting an operational Control Room environment from the HP-UX to Linux platform as well as the many hurdles that had to be overcome throughout the transition period

COOLII code conversion from Cyber to HP

International Nuclear Information System (INIS)

Lee, Hae Cho; Kim, Hee Kyung

1996-06-01

COOLII computer code determines the time required to cooldown the plant from shutdown cooling system initiation condition to cold shutdown or refueling condition. Required time for cooldown is calculated under the various assumption on shutdown cooling heat exchanger(SDCHX) availability, reactor coolant system (RCS) low pressure safety injection(LPSI) flowrate. RCS cooldown rates and component cooling system flow rates. This report firstly describes detailed work carried out for installation of COOLII on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

Teadmata põhjusega haavandtõve tekitaja / iiris Saluri

Index Scriptorium Estoniae

Saluri, iiris

2008-01-01

Pariisis toimunud Euroopa Gastroenteroloogia nädala raames pöörati tähelepanu kaksteistsõrmiksoolehaavandi ja selle peamiste tekitajate - Helicobacter pylori (HP) ning mittesteroidsete põletikuvastaste ravimite tarvitamisele

Functional and evolutionary analyses of Helicobacter pylori HP0231 (DsbK protein with strong oxidative and chaperone activity characterized by a highly diverged dimerization domain

Directory of Open Access Journals (Sweden)

Katarzyna Marta Bocian-Ostrzycka

2015-10-01

Full Text Available Helicobacter pylori does not encode the classical DsbA/DsbB oxidoreductases that are crucial for oxidative folding of extracytoplasmic proteins. Instead, this microorganism encodes an untypical two proteins playing a role in disulfide bond formation – periplasmic HP0231, which structure resembles that of EcDsbC/DsbG, and its redox partner, a membrane protein HpDsbI (HP0595 with a -propeller structure. The aim of presented work was to assess relations between HP0231 structure and function.We showed that HP0231 is most closely related evolutionarily to the catalytic domain of DsbG, even though it possesses a catalytic motif typical for canonical DsbA proteins. Similarly, the highly diverged N-terminal dimerization domain is homologous to the dimerization domain of DsbG. To better understand the functioning of this atypical oxidoreductase, we examined its activity using in vivo and in vitro experiments. We found that HP0231 exhibits oxidizing and chaperone activities but no isomerizing activity, even though H. pylori does not contain a classical DsbC. We also show that HP0231 is not involved in the introduction of disulfide bonds into HcpC (Helicobacter cysteine-rich protein C, a protein involved in the modulation of the H. pylori interaction with its host. Additionally, we also constructed a truncated version of HP0231 lacking the dimerization domain, denoted HP0231m, and showed that it acts in E. coli cells in a DsbB-dependent manner. In contrast, HP0231m and classical monomeric EcDsbA (Escherichia coli DsbA protein were both unable to complement the lack of HP0231 in H. pylori cells, though they exist in oxidized forms. HP0231m is inactive in the insulin reduction assay and possesses high chaperone activity, in contrast to EcDsbA. In conclusion, HP0231 combines oxidative functions characteristic of DsbA proteins and chaperone activity characteristic of DsbC/DsbG, and it lacks isomerization activity.

Metamorphic P-T-t-d evolution of (U)HP metabasites from the South Tianshan accretionary complex (NW China) - Implications for rock deformation during exhumation in a subduction channel

Czech Academy of Sciences Publication Activity Database

Soldner, J.; Oliot, E.; Schulmann, K.; Štípská, P.; Kusbach, Vladimír; Anczkiewicz, R.

2017-01-01

Roč. 47, July (2017), s. 161-187 ISSN 1342-937X Institutional support: RVO:67985530 Keywords : eclogite * Tianshan massif * (U)HP metamorphic belt Subject RIV: DB - Geology ; Mineralogy OBOR OECD: Geology Impact factor: 6.959, year: 2016

Experimental investigation of a PCM-HP heat sink on its thermal performance and anti-thermal-shock capacity for high-power LEDs

International Nuclear Information System (INIS)

Wu, Yuxuan; Tang, Yong; Li, Zongtao; Ding, Xinrui; Yuan, Wei; Zhao, Xuezhi; Yu, Binhai

2016-01-01

Highlights: • A phase-change material (PCM) base heat pipe heat sink (PCM-HP heat sink) is designed. • The PCM-HP heat sink can significantly lower the LED heating rate and temperature. • The PCM-HP heat sink achieves a best anti-thermal-shock capacity in LED cyclic working modes. - Abstract: High-power LEDs demonstrate a number of benefits compared with conventional incandescent lamps and fluorescent lamps, including a longer lifetime, higher brightness and lower power consumption. However, owing to their severe high heat flux, it is difficult to develop effective thermal management of high-power LEDs, especially under cyclic working modes, which cause serious periodic thermal stress and limit further development. Focusing on the above problem, this paper designed a phase-change material (PCM) base heat pipe heat sink (PCM-HP heat sink) that consists of a PCM base, adapter plate, heat pipe and finned radiator. Different parameters, such as three types of interior materials to fill the heat sink, three LED power inputs and eight LED cyclic working modes, were separately studied to investigate the thermal performance and anti-thermal-shock capacity of the PCM-HP heat sink. The results show that the PCM-HP heat sink possesses remarkable thermal performance owing to the reduction of the LED heating rate and peak temperature. More importantly, an excellent anti-thermal-shock capacity of the PCM-HP heat sink is also demonstrated when applied in LED cyclic working modes, and this capacity demonstrates the best range.

Optimization of statistical methods for HpGe gamma-ray spectrometer used in wide count rate ranges

Energy Technology Data Exchange (ETDEWEB)

Gervino, G., E-mail: gervino@to.infn.it [UNITO - Università di Torino, Dipartimento di Fisica, Turin (Italy); INFN - Istituto Nazionale di Fisica Nucleare, Sez. Torino, Turin (Italy); Mana, G. [INRIM - Istituto Nazionale di Ricerca Metrologica, Turin (Italy); Palmisano, C. [UNITO - Università di Torino, Dipartimento di Fisica, Turin (Italy); INRIM - Istituto Nazionale di Ricerca Metrologica, Turin (Italy)

2016-07-11

The need to perform γ-ray measurements with HpGe detectors is a common technique in many fields such as nuclear physics, radiochemistry, nuclear medicine and neutron activation analysis. The use of HpGe detectors is chosen in situations where isotope identification is needed because of their excellent resolution. Our challenge is to obtain the “best” spectroscopy data possible in every measurement situation. “Best” is a combination of statistical (number of counts) and spectral quality (peak, width and position) over a wide range of counting rates. In this framework, we applied Bayesian methods and the Ellipsoidal Nested Sampling (a multidimensional integration technique) to study the most likely distribution for the shape of HpGe spectra. In treating these experiments, the prior information suggests to model the likelihood function with a product of Poisson distributions. We present the efforts that have been done in order to optimize the statistical methods to HpGe detector outputs with the aim to evaluate to a better order of precision the detector efficiency, the absolute measured activity and the spectra background. Reaching a more precise knowledge of statistical and systematic uncertainties for the measured physical observables is the final goal of this research project.

Preliminary assessment of Geant4 HP models and cross section libraries by reactor criticality benchmark calculations

DEFF Research Database (Denmark)

Cai, Xiao-Xiao; Llamas-Jansa, Isabel; Mullet, Steven

2013-01-01

Geant4 is an open source general purpose simulation toolkit for particle transportation in matter. Since the extension of the thermal scattering model in Geant4.9.5 and the availability of the IAEA HP model cross section libraries, it is now possible to extend the application area of Geant4......, U and O in uranium dioxide, Al metal, Be metal, and Fe metal. The native HP cross section library G4NDL does not include data for elements with atomic number larger than 92. Therefore, transuranic elements, which have impacts for a realistic reactor, can not be simulated by the combination of the HP...... models and the G4NDL library. However, cross sections of those missing isotopes were made available recently through the IAEA project “new evaluated neutron cross section libraries for Geant4”....

hpGEM -- A software framework for discontinuous Galerkin finite element methods

NARCIS (Netherlands)

Pesch, L.; Bell, A.; Sollie, W.E.H.; Ambati, V.R.; Bokhove, Onno; van der Vegt, Jacobus J.W.

2006-01-01

hpGEM, a novel framework for the implementation of discontinuous Galerkin finite element methods, is described. We present structures and methods that are common for many (discontinuous) finite element methods and show how we have implemented the components as an object-oriented framework. This

Concentrations of PM2.5-10 and PM2.5 and metallic elements around the Schmidt Stream area, in the Sinos River Basin, southern Brazil

Directory of Open Access Journals (Sweden)

D. D. Alves

Full Text Available This research aimed to evaluate the air quality, by determining the concentrations of PM2.5-10, PM2.5 and the metallic elements Al, Ba, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn and Hg in the leaf part of ryegrass (Lolium multiflorum in an area close to Schmidt Stream, at the lower section of Sinos River Basin (SRB, in a research campaign of six months, from October 2013 to March 2014. The particles collected in the PM sampling were analyzed by Scanning Electron Microscopy (SEM combined with Energy Dispersive X-ray Spectrometry (EDS, in order to study their morphology and chemical composition. The mean concentration of PM2.5-10 was 9.1 µg m–3, with a range of 2.2 µg m–3 to 15.4 µg m–3 and the mean concentration of PM2.5was 4.7 µg m–3, with a range of 1.9 µg m–3 to 8.2 µg m–3. Concentrations of metallic elements, especially Pb, Cr and Zn, were classified as Class 4 (very high pollution levels, according to the classification proposed by Klumpp et al. (2004. Chemical and morphological analysis of PM revealed the presence of particles of biological origin, soot (Cr, Fe, Ni, Zn, Cd, Hg and Pb, salts (KCl and soil resuspension (Al and Si. The integrated study methodology, employing environmental variables, such as PM and ryegrass, can be of help in the preparation of wide-ranging environmental diagnoses, in addition providing information needed to develop precautionary measures designed to minimize the effects of atmospheric pollution that takes into consideration the environment’s supportive capacity and environmental quality.

Spectral/hp element methods for CFD

CERN Document Server

Karniadakis, George Em

1999-01-01

Traditionally spectral methods in fluid dynamics were used in direct and large eddy simulations of turbulent flow in simply connected computational domains. The methods are now being applied to more complex geometries, and the spectral/hp element method, which incorporates both multi-domain spectral methods and high-order finite element methods, has been particularly successful. This book provides a comprehensive introduction to these methods. Written by leaders in the field, the book begins with a full explanation of fundamental concepts and implementation issues. It then illustrates how these methods can be applied to advection-diffusion and to incompressible and compressible Navier-Stokes equations. Drawing on both published and unpublished material, the book is an important resource for experienced researchers and for those new to the field.

HP-HMG versus rFSH in treatments combining fresh and frozen IVF cycles: success rates and economic evaluation.

Science.gov (United States)

Wex-Wechowski, Jaro; Abou-Setta, Ahmed M; Kildegaard Nielsen, Sandy; Kennedy, Richard

2010-08-01

The economic implications of the choice of gonadotrophin influence decision making but their cost-effectiveness in frozen-embryo transfer cycles has not been adequately studied. An economic evaluation was performed comparing highly purified human menopausal gonadotrophin (HP-HMG) and recombinant FSH (rFSH) using individual patient data (n=986) from two large randomized controlled trials using a long agonist IVF protocol. The simulation model incorporated live birth data and published UK costs of IVF-related medical resources. After treatment for up-to-three cycles (one fresh and up to two subsequent fresh or frozen cycles conditional on availability of cryopreserved embryos), the cumulative live birth rate was 53.7% (95% CI 49.3-58.1%) for HP-HMG and 44.6% (40.2-49.0%) for rFSH (OR 1.44, 95% CI 1.12-1.85; Pcosts per IVF treatment for HP-HMG and rFSH were pound5393 ( pound5341-5449) and pound6269 ( pound6210-6324), respectively (number needed to treat to fund one additional treatment was seven; Pcosts applied, the median cost per IVF baby delivered with HP-HMG was pound11,157 ( pound11,089-11,129) and pound14,227 ( pound14,183-14,222) with rFSH (Pcost saving using HP-HMG remained after varying model parameters in a probabilistic sensitivity analysis. 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

The Weak Link HP-41C hand-held calculator program

Science.gov (United States)

Ross A. Phillips; Penn A. Peters; Gary D. Falk

1982-01-01

The Weak Link hand-held calculator program (HP-41C) quickly analyzes a system for logging production and costs. The production equations model conventional chain saw, skidder, loader, and tandemaxle truck operations in eastern mountain areas. Production of each function of the logging system may be determined so that the system may be balanced for minimum cost. The...

Heterochromatin marks HP1γ, HP1α and H3K9me3, and DNA damage response activation in human testis development and germ cell tumours

DEFF Research Database (Denmark)

Bartkova, J; Moudry, P; Hodny, Z

2011-01-01

activation in CIS; and enhanced HP1α in teratoma structures with epithelial and neuronal differentiation. Differential expression of the three heterochromatin markers suggests their partly non-overlapping roles, and separation of heterochromatinization from DDR activation highlights distinct responses...

Mutation of charged residues to neutral ones accelerates urea denaturation of HP-35.

Science.gov (United States)

Wei, Haiyan; Yang, Lijiang; Gao, Yi Qin

2010-09-16

Following the studies of urea denaturation of β-hairpins using molecular dynamics, in this paper, molecular dynamics simulations of two peptides, a 35 residue three helix bundle villin headpiece protein HP-35 and its doubly norleucine-substituent mutant (Lys24Nle/Lys29Nle) HP-35 NleNle, were undertaken in urea solutions to understand the molecular mechanism of urea denaturation of α-helices. The mutant HP-35 NleNle was found to denature more easily than the wild type. During the expansion of the small hydrophobic core, water penetration occurs first, followed by that of urea molecules. It was also found that the initial hydration of the peptide backbone is achieved through water hydrogen bonding with the backbone CO groups during the denaturation of both polypeptides. The mutation of the two charged lysine residues to apolar norleucine enhances the accumulation of urea near the hydrophobic core and facilitates the denaturation process. Urea also interacts directly with the peptide backbone as well as side chains, thereby stabilizing nonnative conformations. The mechanism revealed here is consistent with the previous study on secondary structure of β-hairpin polypeptide, GB1, PEPTIDE 1, and TRPZIP4, suggesting that there is a general mechanism in the denaturation of protein backbone hydrogen bonds by urea.

Comparison of gadolinium Cy2DOTA, a new hepatobiliary agent, and gadolinium HP-DO3A, an extracellular agent, in healthy liver and metastatic disease

International Nuclear Information System (INIS)

Runge, V.M.; Wells, J.W.; Williams, N.M.

1995-01-01

A new gadolinium (Gd) chelate with preferential hepatobiliary uptake, Gd Cy 2 DOTA, was compared in two animal species with Gd HP-DO3A (gadoteridol), a clinically approved contrast agent with extracellular distribution. Liver enhancement was evaluated for these two contrast agents using magnetic resonance imaging, whereas an experimental model of metastatic disease was used to evaluate the agents' efficacy for liver-lesion delineation. The two agents were compared in four healthy Rhesus monkeys (eight studies) and five New Zealand White rabbits with implanted VX-2 liver tumors (ten studies). The contrast dose was 0.1 mmol/kg, with the agents given in random order and at least 72 hours between contrast injections. Breathhold T1-weighted spin echo scans were obtained at 1.5 tesla (T) before and after contrast was administered. Postcontrast scans were obtained 1 to 90 minutes after injection in the monkeys and 1 to 240 minutes after injection in the rabbits. Prolonged hepatic enhancement, superior in degree to that with Gd HP-DO3A, was noted to both monkeys and rabbits after injection of Gd Cy 2 DOTA. Two minutes after contrast, liver SI was 1.94 ± 0.05 with Gd Cy 2 DOTA compared with 1.5 ± 0.05 with Gd HP-DO3A in monkeys. Sixty minutes after contrast, liver SI was 1.60 ± 0.09 compared with 1.20 ± 0.02. The difference between agents was significant at all times from 2 to 60 minutes after contrast injection (P 2 DOTA but not with Gd HP-DO3A. The maximum improvement in lesion conspicuity (rabbit) occurred 45 minutes after injection of Gd Cy 2 DOTA and 5 minutes after injection of Gd HP-DO3A. 22 refs., 12 figs

The cag PAI is intact and functional but HP0521 varies significantly in Helicobacter pylori isolates from Malaysia and Singapore.

Science.gov (United States)

Schmidt, H-M A; Andres, S; Nilsson, C; Kovach, Z; Kaakoush, N O; Engstrand, L; Goh, K-L; Fock, K M; Forman, D; Mitchell, H

2010-04-01

Helicobacter pylori-related disease is at least partially attributable to the genotype of the infecting strain, particularly the presence of specific virulence factors. We investigated the prevalence of a novel combination of H. pylori virulence factors, including the cag pathogenicity island (PAI), and their association with severe disease in isolates from the three major ethnicities in Malaysia and Singapore, and evaluated whether the cag PAI was intact and functional in vitro. Polymerase chain reaction (PCR) was used to detect dupA, cagA, cagE, cagT, cagL and babA, and to type vacA, the EPIYA motifs, HP0521 alleles and oipA ON status in 159 H. pylori clinical isolates. Twenty-two strains were investigated for IL-8 induction and CagA translocation in vitro. The prevalence of cagA, cagE, cagL, cagT, babA, oipA ON and vacA s1 and i1 was >85%, irrespective of the disease state or ethnicity. The prevalence of dupA and the predominant HP0521 allele and EPIYA motif varied significantly with ethnicity (p < 0.05). A high prevalence of an intact cag PAI was found in all ethnic groups; however, no association was observed between any virulence factor and disease state. The novel association between the HP0521 alleles, EPIYA motifs and host ethnicity indicates that further studies to determine the function of this gene are important.

Improved Functional Properties and Efficiencies of Nitinol Wires Under High-Performance Shape Memory Effect (HP-SME)

Science.gov (United States)

Casati, R.; Saghafi, F.; Biffi, C. A.; Vedani, M.; Tuissi, A.

2017-10-01

Martensitic Ti-rich NiTi intermetallics are broadly used in various cyclic applications as actuators, which exploit the shape memory effect (SME). Recently, a new approach for exploiting austenitic Ni-rich NiTi shape memory alloys as actuators was proposed and named high-performance shape memory effect (HP-SME). HP-SME is based on thermal recovery of de-twinned martensite produced by mechanical loading of the parent phase. The aim of the manuscript consists in evaluating and comparing the fatigue and actuation properties of austenitic HP-SME wires and conventional martensitic SME wires. The effect of the thermomechanical cycling on the actuation response and the changes in the electrical resistivity of both shape memory materials were studied by performing the actuation tests at different stages of the fatigue life. Finally, the changes in the transition temperatures before and after cycling were also investigated by differential calorimetric tests.

Structural effects at Λ3Hp-scattering

International Nuclear Information System (INIS)

Tartakovskij, V.K.; Fursaev, A.V.; Ivanova, O.I.

2003-01-01

In the paper the calculated dependences of falling hyper-tritons energy E=156 MeV differential cross sections from the scattering angle in the center of mass system θ and for more high energy values E from coupling energy ε 0 of Λ 3 H with regard to its disintegration into Λ-hyperon and deuteron are presented. It is established the strong dependence of differential cross section from ε 0 , moreover the sensitivity of differential cross sections to ε 0 value grows up with increasing of energy E. This allows to use the Λ 3 Hp-scattering as a method for ε 0 value elaboration

On optimal node and polynomial degree distribution in one-dimensional hp -FEM

Czech Academy of Sciences Publication Activity Database

Chleboun, J.; Šolín, Pavel

2013-01-01

Roč. 95, č. 1 (2013), s. 75-88 ISSN 1436-5057 R&D Projects: GA ČR GAP105/10/1682 Institutional support: RVO:61388998 Keywords : hp * boundary value problem * optimal mesh Subject RIV: JM - Building Engineering

A new crystal lattice structure of Helicobacter pylori neutrophil-activating protein (HP-NAP)

International Nuclear Information System (INIS)

Tsuruta, Osamu; Yokoyama, Hideshi; Fujii, Satoshi

2012-01-01

A new crystal lattice structure of H. pylori neutrophil-activating protein has been determined. Iron loading causes a series of conformational changes at the ferroxidase centre. A new crystal lattice structure of Helicobacter pylori neutrophil-activating protein (HP-NAP) has been determined in two forms: the native state (Apo) at 2.20 Å resolution and an iron-loaded form (Fe-load) at 2.50 Å resolution. The highly solvated packing of the dodecameric shell is suitable for crystallographic study of the metal ion-uptake pathway. Like other bacterioferritins, HP-NAP forms a spherical dodecamer with 23 symmetry including two kinds of channels. Iron loading causes a series of conformational changes of amino-acid residues (Trp26, Asp52 and Glu56) at the ferroxidase centre

Hp Ge: Purification, crystal growth, and annealing properties

International Nuclear Information System (INIS)

Hall, R.N.

1984-01-01

The prospects for growing HP Ge crystals of increased size and purity are examined. One interesting approach is to grow dislocation-free crystals, which must then be annealed to reduce the concentration of V 2 H traps. The phenomena which occur during annealing are discussed and compared with experiment. Hydrogen, present in atomic form at the growth temperature, forms H 2 molecules during cooling, causing the effective diffusion coefficient to decrease rapidly. Models representing the reactions between H and the V 2 H, A(H, Si), and D(H,O) complexes are presented and analyzed

Comparative analysis of the folding dynamics and kinetics of an engineered knotted protein and its variants derived from HP0242 of Helicobacter pylori

Science.gov (United States)

Wang, Liang-Wei; Liu, Yu-Nan; Lyu, Ping-Chiang; Jackson, Sophie E.; Hsu, Shang-Te Danny

2015-09-01

Understanding the mechanism by which a polypeptide chain thread itself spontaneously to attain a knotted conformation has been a major challenge in the field of protein folding. HP0242 is a homodimeric protein from Helicobacter pylori with intertwined helices to form a unique pseudo-knotted folding topology. A tandem HP0242 repeat has been constructed to become the first engineered trefoil-knotted protein. Its small size renders it a model system for computational analyses to examine its folding and knotting pathways. Here we report a multi-parametric study on the folding stability and kinetics of a library of HP0242 variants, including the trefoil-knotted tandem HP0242 repeat, using far-UV circular dichroism and fluorescence spectroscopy. Equilibrium chemical denaturation of HP0242 variants shows the presence of highly populated dimeric and structurally heterogeneous folding intermediates. Such equilibrium folding intermediates retain significant amount of helical structures except those at the N- and C-terminal regions in the native structure. Stopped-flow fluorescence measurements of HP0242 variants show that spontaneous refolding into knotted structures can be achieved within seconds, which is several orders of magnitude faster than previously observed for other knotted proteins. Nevertheless, the complex chevron plots indicate that HP0242 variants are prone to misfold into kinetic traps, leading to severely rolled-over refolding arms. The experimental observations are in general agreement with the previously reported molecular dynamics simulations. Based on our results, kinetic folding pathways are proposed to qualitatively describe the complex folding processes of HP0242 variants.

Ant colony optimization techniques for the hamiltonian p-median problem

Directory of Open Access Journals (Sweden)

M. Zohrehbandian

2010-12-01

Full Text Available Location-Routing problems involve locating a number of facilitiesamong candidate sites and establishing delivery routes to a set of users in such a way that the total system cost is minimized. A special case of these problems is Hamiltonian p-Median problem (HpMP. This research applies the metaheuristic method of ant colony optimization (ACO to solve the HpMP. Modifications are made to the ACO algorithm used to solve the traditional vehicle routing problem (VRP in order to allow the search of the optimal solution of the HpMP. Regarding this metaheuristic algorithm a computational experiment is reported as well.

Discrete frequency identification using the HP 5451B Fourier analyser

International Nuclear Information System (INIS)

Holland, L.; Barry, P.

1977-01-01

The frequency analysis by the HP5451B discrete frequency Fourier analyser is studied. The advantages of cross correlation analysis to identify discrete frequencies in a background noise are discussed in conjuction with the elimination of aliasing and wraparound error. Discrete frequency identification is illustrated by a series of graphs giving the results of analysing 'electrical' and 'acoustical' white noise and sinusoidal signals [pt

Evaluation of austenitic stainless steels for transpassive corrosion by metal purification technology. Synergistic effect of Si and P on intergranular corrosion of Fe-18Cr-14Ni alloys

International Nuclear Information System (INIS)

Mayuzumi, Masami; Ohta, Joji; Kako, Kenji; Kawakami, Eishi

2001-01-01

The synergistic effect of Si, Mn, C, P, and S on the transpassive corrosion of HP18Cr-14Ni alloys was studied in 13N nitric acid. The specimens were fabricated using a cold crucible method in a high-vacuum chamber to reduce contamination. The additions of Si<1% and Mn<2% had no effect on the corrosion behavior of HP18Cr-14Ni alloys, and the addition of Si<1% also had no effect on the corrosion behavior of HP18Cr-14Ni-1Mn alloys, although 1% Si induced intergranular corrosion in both the alloys. Thus, HP18Cr-14Ni-1Mn-0.5Si alloys were selected to evaluate the effects of C, P and S (100 ppm each). The addition of P, and the co-addition of C, P, and S to HP18Cr-14Ni-1Mn-0.5Si induced intergranular corrosion of the same degree in the solution annealed condition. This result suggests the synergistic effect of Si and P to induce intergranular corrosion, since the single addition of Si or P to this level did not lead to intergranular corrosion of HP18Cr-14Ni alloys. HP18Cr-14Ni-1Mn-0.5Si alloys containing C, P, and S at the 100 ppm level each showed superior corrosion resistance compared to a commercial Type 304L in 13N nitric acid. (author)

Crystallography and Morphology of MC Carbides in Niobium-Titanium Modified As-Cast HP Alloys

Science.gov (United States)

Buchanan, Karl G.; Kral, Milo V.; Bishop, Catherine M.

2014-07-01

The microstructures of two as-cast heats of HP alloy stainless steels modified with niobium and titanium were examined with particular attention paid to the interdendritic niobium-titanium-rich carbides formed during solidification of these alloys. Generally, these precipitates obtain a blocky morphology in the as-cast condition. However, the (NbTi)C precipitates may obtain a nodular morphology. To provide further insight to the origin of the two different morphologies obtained by the (NbTi)C precipitates in the HP-NbTi alloy, the microstructure and crystallography of each have been studied in detail using scanning electron microscopy, transmission electron microscopy, various electron diffraction methods (EBSD, SAD, and CBED), and energy-dispersive X-ray spectroscopy.

Surface adsorption of lattice HP proteins: Thermodynamics and structural transitions using Wang-Landau sampling

International Nuclear Information System (INIS)

Li Yingwai; Landau, David P; Wüst, Thomas

2012-01-01

Wang-Landau sampling has been applied to investigate the thermodynamics and structural properties of a lattice hydrophobic-polar heteropolymer (the HP protein model) interacting with an attractive substrate. For simplicity, we consider a short HP sequence consisting of only 36 monomers interacting with a substrate which attracts all monomers in the sequence. The conformational “phase transitions” have been identified by a canonical analysis of the specific heat and suitable structural observables. Three major “transitions”, namely, adsorption, hydrophobic core formation and “flattening” of adsorbed structures, are observed. Depending on the surface attractive strength relative to the intra-protein attraction among the H monomers, these processes take place in different sequences upon cooling.

COAST code conversion from Cyber to HP

International Nuclear Information System (INIS)

Lee, Hae Cho

1996-04-01

The transient thermal hydraulic behavior of reactor coolant system in a nuclear power plant following loss of coolant flow is analyzed by use of COAST digital computer code. COAST calculates individual loop flow rates and steam generator pressure drops is a function of time following coast-down of any number of reactor coolant pumps. This report firstly describes detailed work carried out for installation of COAST on HP 9000/700 series and code validation results after installation. Secondly, a series of work is also describes in relation to installation of COAST on Apollo DN10000 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

Modeling of heterogeneous elastic materials by the multiscale hp-adaptive finite element method

Science.gov (United States)

Klimczak, Marek; Cecot, Witold

2018-01-01

We present an enhancement of the multiscale finite element method (MsFEM) by combining it with the hp-adaptive FEM. Such a discretization-based homogenization technique is a versatile tool for modeling heterogeneous materials with fast oscillating elasticity coefficients. No assumption on periodicity of the domain is required. In order to avoid direct, so-called overkill mesh computations, a coarse mesh with effective stiffness matrices is used and special shape functions are constructed to account for the local heterogeneities at the micro resolution. The automatic adaptivity (hp-type at the macro resolution and h-type at the micro resolution) increases efficiency of computation. In this paper details of the modified MsFEM are presented and a numerical test performed on a Fichera corner domain is presented in order to validate the proposed approach.

An hp-local Discontinuous Galerkin Method for Parabolic Integro-Differential Equations

KAUST Repository

Pani, Amiya K.

2010-06-06

In this article, a priori error bounds are derived for an hp-local discontinuous Galerkin (LDG) approximation to a parabolic integro-differential equation. It is shown that error estimates in L 2-norm of the gradient as well as of the potential are optimal in the discretizing parameter h and suboptimal in the degree of polynomial p. Due to the presence of the integral term, an introduction of an expanded mixed type Ritz-Volterra projection helps us to achieve optimal estimates. Further, it is observed that a negative norm estimate of the gradient plays a crucial role in our convergence analysis. As in the elliptic case, similar results on order of convergence are established for the semidiscrete method after suitably modifying the numerical fluxes. The optimality of these theoretical results is tested in a series of numerical experiments on two dimensional domains. © 2010 Springer Science+Business Media, LLC.

An hp-local Discontinuous Galerkin Method for Parabolic Integro-Differential Equations

KAUST Repository

Pani, Amiya K.; Yadav, Sangita

2010-01-01

In this article, a priori error bounds are derived for an hp-local discontinuous Galerkin (LDG) approximation to a parabolic integro-differential equation. It is shown that error estimates in L 2-norm of the gradient as well as of the potential are optimal in the discretizing parameter h and suboptimal in the degree of polynomial p. Due to the presence of the integral term, an introduction of an expanded mixed type Ritz-Volterra projection helps us to achieve optimal estimates. Further, it is observed that a negative norm estimate of the gradient plays a crucial role in our convergence analysis. As in the elliptic case, similar results on order of convergence are established for the semidiscrete method after suitably modifying the numerical fluxes. The optimality of these theoretical results is tested in a series of numerical experiments on two dimensional domains. © 2010 Springer Science+Business Media, LLC.

Investigation of the three-dimensional lattice HP protein folding model using a genetic algorithm

Directory of Open Access Journals (Sweden)

Fábio L. Custódio

2004-01-01

Full Text Available An approach to the hydrophobic-polar (HP protein folding model was developed using a genetic algorithm (GA to find the optimal structures on a 3D cubic lattice. A modification was introduced to the scoring system of the original model to improve the model's capacity to generate more natural-like structures. The modification was based on the assumption that it may be preferable for a hydrophobic monomer to have a polar neighbor than to be in direct contact with the polar solvent. The compactness and the segregation criteria were used to compare structures created by the original HP model and by the modified one. An islands' algorithm, a new selection scheme and multiple-points crossover were used to improve the performance of the algorithm. Ten sequences, seven with length 27 and three with length 64 were analyzed. Our results suggest that the modified model has a greater tendency to form globular structures. This might be preferable, since the original HP model does not take into account the positioning of long polar segments. The algorithm was implemented in the form of a program with a graphical user interface that might have a didactical potential in the study of GA and on the understanding of hydrophobic core formation.

The tensor analyzing power T20 in the dd → 3Hen and dd → 3Hp reactions at the energies 140, 200 and 270 MeV and at zero degree

International Nuclear Information System (INIS)

Ladygin, V.P.; Uesaka, T.; Saito, T.

2005-01-01

The data on the tensor analyzing power T 20 in the dd → 3 Hen and dd → 3 Hp reactions at 140, 200 and 270 MeV of the deuteron kinetic energy and at zero degree obtained at RIKEN Accelerator Research Facility are presented. The observed positive sign of T 20 clearly demonstrates the sensitivity to the D/S wave ratios in the 3 He and 3 H in the energy domain of the measurements. The T 20 data for the 3 He-n and 3 H-p channels are in agreement within experimental accuracy

SGN III code conversion from Apollo to HP

International Nuclear Information System (INIS)

Lee, Hae Cho

1996-04-01

SGN III computer code is used to analyze transient behavior of reactor coolant system, pressurizer and steam generators in the event of main steam line break (MSLB), and to calculate mass and energy release for containment design. This report firstly describes detailed work carried out for installation of SFN III on Apollo DN 10000 and code validation results after installation. Secondly, a series of work is also describes in relation to installation of SGN III on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 8 refs. (Author) .new

CONTRANS 2 code conversion from Apollo to HP

International Nuclear Information System (INIS)

Lee, Hae Cho

1996-01-01

CONTRANS2 computer code is used to calculate transient thermal hydraulic responses of containment building to loss of coolant and main steam line break accident. Mass and energy release to the containment following an accident are code inputs. This report firstly describes detailed work carried out for installation of CONTRANS2 on Apollo DN10000 and code validation results after installation. Secondly, A series of work is also describes in relation to installation of CONTRANS2 on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

PULSION registered HP: Tunable, High Productivity Plasma Doping

International Nuclear Information System (INIS)

Felch, S. B.; Torregrosa, F.; Etienne, H.; Spiegel, Y.; Roux, L.; Turnbaugh, D.

2011-01-01

Plasma doping has been explored for many implant applications for over two decades and is now being used in semiconductor manufacturing for two applications: DRAM polysilicon counter-doping and contact doping. The PULSION HP is a new plasma doping tool developed by Ion Beam Services for high-volume production that enables customer control of the dominant mechanism--deposition, implant, or etch. The key features of this tool are a proprietary, remote RF plasma source that enables a high density plasma with low chamber pressure, resulting in a wide process space, and special chamber and wafer electrode designs that optimize doping uniformity.

Scoring the home falls and accidents screening tool for health professionals (HOME FAST-HP): Evidence from one epidemiological study.

Science.gov (United States)

Mackenzie, Lynette; Byles, Julie

2018-03-30

Falls in older people are a major public health concern. To target falls prevention interventions, screening tools need to be able to identify older people at greater risk of falling. This study aimed to investigate the screening capacity of the Home Falls and Accidents Screening Tool for health professionals (HOME FAST-HP), and to identify the best cut-off score to identify older people at higher risk of falls using the HOME FAST-HP. The study used cross-sectional data from a random sample of 650 women from the 1921 to 1926 cohort of the Australian Longitudinal Study of Women's Health (ALSWH). Selected women were sent a postal survey including the HOME FAST-HP, falls history, and other health factors. Scores on the home fast were calculated and the cut-point for optimal sensitivity and specificity of the HOME FAST-HP in relation to falls was assessed using a Receiver Operating Characteristic curve. A total of 567 older women participated (response rate 87%). The mean age of participants was 77.5 yrs (95% CI 77.31-77.70). A total of 153 participants (27%) reported a fall in the previous six months. The mean number of hazards using the HOME FAST-HP was 9.74 (95% CI 9.48-10.01), range 2-22. Non-fallers had a mean of 9.6 hazards (95% CI 9.32-9.91) and fallers had a mean of 10.63 hazards (95% CI 10.08-11.19) which was a significant difference (t = 3.41, P = 0.001). The area under the receiver operator curve (AUC) was 0.58 (95% CI 0.53-0.64). A HOME FAST-HP cut-off score of 9 was associated with the optimal sensitivity for falls (73.9%), with specificity (37.9%), and positive predictive value was 30.6% and negative predictive value was 79.7%. The HOME FAST-HP can be used as a screening tool to identify fallers with a cut-off score of nine indicating a higher risk of falling. © 2018 Occupational Therapy Australia.

Figure 1. Prediction of ScHP1 transmembrane domains I to XIV. (http ...

Indian Academy of Sciences (India)

Figure 2. Comparison of the ScHP1 amino acid sequence with H. +. -PPase from other species. The conserved domains GGG,. DVGADLVGKVE, DNVGDNVGD, EYYT and GNTTAA were found in the sequence alignment (shown in red boxes).

The HP 9825 A program for the determination of an isotope half-life

International Nuclear Information System (INIS)

Jujuratisbela, Uju.

1980-01-01

Determination of half life of gold 198, indium 116, manganese 56, copper 64 are presented, using least squares method in HP 9825 A. This method is more accurate and needs less time than graphic method. (author tr.)

HP1330 Contributes to Streptococcus suis Virulence by Inducing Toll-Like Receptor 2- and ERK1/2-Dependent Pro-inflammatory Responses and Influencing In Vivo S. suis Loads

Directory of Open Access Journals (Sweden)

Qiang Zhang

2017-07-01

Full Text Available Streptococcus suis 2 (SS2 has evolved into a highly invasive pathogen responsible for two large-scale outbreaks of streptococcal toxic shock-like syndrome (STSLS in China. Excessive inflammation stimulated by SS2 is considered a hallmark of STSLS, even it also plays important roles in other clinical symptoms of SS2-related disease, including meningitis, septicemia, and sudden death. However, the mechanism of SS2-caused excessive inflammation remains poorly understood. Here, a novel pro-inflammatory protein was identified (HP1330, which could induce robust expression of pro-inflammatory cytokines (TNF-α, MCP-1, and IL-1β in RAW264.7 macrophages. To evaluate the role of HP1330 in SS2 virulence, an hp1330-deletion mutant (Δhp1330 was constructed. In vitro, hp1330 disruption led to a decreased pro-inflammatory ability of SS2 in RAW 264.7 macrophages. In vivo, Δhp1330 showed reduced lethality, pro-inflammatory activity, and bacterial loads in mice. To further elucidate the mechanism of HP1330-induced pro-inflammatory cytokine production, antibody blocking and gene-deletion experiments with macrophages were performed. The results revealed that the pro-inflammatory activity of HP1330 depended on the recognition of toll-like receptor 2 (TLR2. Furthermore, a specific inhibitor of the extracellular signal-regulated kinase 1/2 (ERK1/2 pathways could significantly decrease HP1330-induced pro-inflammatory cytokine production, and western blot analysis showed that HP1330 could induce activation of the ERK1/2 pathway. Taken together, our findings demonstrate that HP1330 contributes to SS2 virulence by inducing TLR2- and ERK1/2-dependent pro-inflammatory cytokine production and influencing in vivo bacterial loads, implying that HP1330 may be associated with STSLS caused by SS2.

Assessment of reproductive and developmental effects of DINP, DnHP and DCHP using quantitative weight of evidence.

Science.gov (United States)

Dekant, Wolfgang; Bridges, James

2016-11-01

Quantitative weight of evidence (QWoE) methodology utilizes detailed scoring sheets to assess the quality/reliability of each publication on toxicity of a chemical and gives numerical scores for quality and observed toxicity. This QWoE-methodology was applied to the reproductive toxicity data on diisononylphthalate (DINP), di-n-hexylphthalate (DnHP), and dicyclohexylphthalate (DCHP) to determine if the scientific evidence for adverse effects meets the requirements for classification as reproductive toxicants. The scores for DINP were compared to those when applying the methodology DCHP and DnHP that have harmonized classifications. Based on the quality/reliability scores, application of the QWoE shows that the three databases are of similar quality; but effect scores differ widely. Application of QWoE to DINP studies resulted in an overall score well below the benchmark required to trigger classification. For DCHP, the QWoE also results in low scores. The high scores from the application of the QWoE methodology to the toxicological data for DnHP represent clear evidence for adverse effects and justify a classification of DnHP as category 1B for both development and fertility. The conclusions on classification based on the QWoE are well supported using a narrative assessment of consistency and biological plausibility. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Tinker-HP: a massively parallel molecular dynamics package for multiscale simulations of large complex systems with advanced point dipole polarizable force fields.

Science.gov (United States)

Lagardère, Louis; Jolly, Luc-Henri; Lipparini, Filippo; Aviat, Félix; Stamm, Benjamin; Jing, Zhifeng F; Harger, Matthew; Torabifard, Hedieh; Cisneros, G Andrés; Schnieders, Michael J; Gresh, Nohad; Maday, Yvon; Ren, Pengyu Y; Ponder, Jay W; Piquemal, Jean-Philip

2018-01-28

We present Tinker-HP, a massively MPI parallel package dedicated to classical molecular dynamics (MD) and to multiscale simulations, using advanced polarizable force fields (PFF) encompassing distributed multipoles electrostatics. Tinker-HP is an evolution of the popular Tinker package code that conserves its simplicity of use and its reference double precision implementation for CPUs. Grounded on interdisciplinary efforts with applied mathematics, Tinker-HP allows for long polarizable MD simulations on large systems up to millions of atoms. We detail in the paper the newly developed extension of massively parallel 3D spatial decomposition to point dipole polarizable models as well as their coupling to efficient Krylov iterative and non-iterative polarization solvers. The design of the code allows the use of various computer systems ranging from laboratory workstations to modern petascale supercomputers with thousands of cores. Tinker-HP proposes therefore the first high-performance scalable CPU computing environment for the development of next generation point dipole PFFs and for production simulations. Strategies linking Tinker-HP to Quantum Mechanics (QM) in the framework of multiscale polarizable self-consistent QM/MD simulations are also provided. The possibilities, performances and scalability of the software are demonstrated via benchmarks calculations using the polarizable AMOEBA force field on systems ranging from large water boxes of increasing size and ionic liquids to (very) large biosystems encompassing several proteins as well as the complete satellite tobacco mosaic virus and ribosome structures. For small systems, Tinker-HP appears to be competitive with the Tinker-OpenMM GPU implementation of Tinker. As the system size grows, Tinker-HP remains operational thanks to its access to distributed memory and takes advantage of its new algorithmic enabling for stable long timescale polarizable simulations. Overall, a several thousand-fold acceleration over

Effect of a long-chained fructan Raftiline HP on blood lipids and spontaneous atherosclerosis in low density receptor knockout mice

DEFF Research Database (Denmark)

Mortensen, Alicja; Poulsen, Morten; Frandsen, Henrik Lauritz

2002-01-01

The effect of a long-chained fructan Raftiline HP on spontaneous hypercholesterolemia and atherosclerosis was studied in 40 LDLR-/- male mice receiving isocaloric, balanced in fat content, purified diets with 0 or 10% Raftiline HP, for 16 weeks. The feed intake was comparable (3.9 v. 3.8 g/day) b...

Test of ring, eye lens and whole body dosemeters for the dose quantity Hp(3) to be used in interventional radiology

Science.gov (United States)

Szumska, A.; Budzanowski, M.; Kopeć, R.

2017-11-01

In its statement on tissue reactions approved on 21st April 2011, the International Commission on Radiological Protection (ICRP, 2012) reviewed its recommendation concerning the equivalent dose limit for the eye lens and reduced the dose limits for occupationally exposed persons to 20 mSv in a year, averaged over defined periods of 5 years, with no single year exceeding 50 mSv. This limit was approved and written down in the new EURATOM (European Atomic Energy Community) directive 2013/59 and in the IAEA (International Atomic Energy Agency) BSS (Basic Safety Standard) of July 2014. For that reason, the necessity to monitor the eye lens may become more important than it was before. However, specially dedicated dosemeters for the dose quantity Hp(3) are using very rarely. Commonly use are only whole body personal dosemeters for the personal dose equivalent quantities Hp(10) worn on the trunk and ring dosemeters worn on finger to measure the quantity Hp(0.07). Therefore, in this work it was investigated whether dosemeters from routine use calibrated in terms of Hp(10) and Hp(0.07) and worn on thyroid collar and protective apron could deliver similar results like dedicated eye lens dosemeter worn close to the eyes. The results show that the best method if dedicated eye lens dosimeters is not used is to measure doses in terms of Hp(0.07) on the thyroid collar (Pearson product, r=0.85). Obtained results shows also importance of proper localization of eye lens dosimeter (close to the eye, from side of the X-ray source).

A CAMAC seven-crate system on-line with an HP2116B computer

International Nuclear Information System (INIS)

Chernenko, S.P.; Chernykh, E.V.; Filatova, N.A.; Ivanov, A.B.; Kolpakov, I.F.; Nikityuk, N.N.; Smirnov, V.A.; Zanevsky, Yu.V.

1975-01-01

A branch of seven CAMAC on-line with an HP2116B computer is described. This system is used for data acquisition from a Cherenkov 90-channel spectrometer, 32 magnetostrictive chambers, 20 registers of the proportional chambers, scalers, and hodoscope registers

Epigenetic aspects of HP1 exchange kinetics in apoptotic chromatin

Czech Academy of Sciences Publication Activity Database

Legartová, Soňa; Jugová, Alžbeta; Stixová, Lenka; Kozubek, Stanislav; Fojtová, Miloslava; Zdráhal, Z.; Lochmanová, G.; Bártová, Eva

2013-01-01

Roč. 95, č. 2 (2013), s. 167-179 ISSN 0300-9084 R&D Projects: GA ČR(CZ) GAP302/10/1022; GA ČR(CZ) GBP302/12/G157; GA MŠk(CZ) LD11020; GA MŠk(CZ) ED1.1.00/02.0068 Institutional research plan: CEZ:AV0Z50040702 Institutional support: RVO:68081707 Keywords : Apoptosis * Histones * HP1 Subject RIV: BO - Biophysics Impact factor: 3.123, year: 2013

Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl.

Science.gov (United States)

Wragg, David; Mwacharo, Joram M; Alcalde, José A; Wang, Chen; Han, Jian-Lin; Gongora, Jaime; Gourichon, David; Tixier-Boichard, Michèle; Hanotte, Olivier

2013-01-01

Oocyan or blue/green eggshell colour is an autosomal dominant trait found in native chickens (Mapuche fowl) of Chile and in some of their descendants in European and North American modern breeds. We report here the identification of an endogenous avian retroviral (EAV-HP) insertion in oocyan Mapuche fowl and European breeds. Sequencing data reveals 100% retroviral identity between the Mapuche and European insertions. Quantitative real-time PCR analysis of European oocyan chicken indicates over-expression of the SLCO1B3 gene (P<0.05) in the shell gland and oviduct. Predicted transcription factor binding sites in the long terminal repeats (LTR) indicate AhR/Ar, a modulator of oestrogen, as a possible promoter/enhancer leading to reproductive tissue-specific over-expression of the SLCO1B3 gene. Analysis of all jungle fowl species Gallus sp. supports the retroviral insertion to be a post-domestication event, while identical LTR sequences within domestic chickens are in agreement with a recent de novo mutation.

Comparing Hp(3) evaluated from the conversion coefficients from air kerma to personal dose equivalent for eye lens dosimetry calibrated on a new cylindrical PMMA phantom

Science.gov (United States)

Esor, J.; Sudchai, W.; Monthonwattana, S.; Pungkun, V.; Intang, A.

2017-06-01

Based on a new occupational dose limit recommended by ICRP (2011), the annual dose limit for the lens of the eye for workers should be reduced from 150 mSv/y to 20 mSv/y averaged over 5 consecutive years in which no single year exceeding 50 mSv. This new dose limit directly affects radiologists and cardiologists whose work involves high radiation exposure over 20 mSv/y. Eye lens dosimetry (Hp(3)) has become increasingly important and should be evaluated directly based on dosimeters that are worn closely to the eye. Normally, Hp(3) dose algorithm was carried out by the combination of Hp(0.07) and Hp(10) values while dosimeters were calibrated on slab PMMA phantom. Recently, there were three reports from European Union that have shown the conversion coefficients from air kerma to Hp(3). These conversion coefficients carried out by ORAMED, PTB and CEA Saclay projects were performed by using a new cylindrical head phantom. In this study, various delivered doses were calculated using those three conversion coefficients while nanoDot, small OSL dosimeters, were used for Hp(3) measurement. These calibrations were performed with a standard X-ray generator at Secondary Standard Dosimetry Laboratory (SSDL). Delivered doses (Hp(3)) using those three conversion coefficients were compared with Hp(3) from nanoDot measurements. The results showed that percentage differences between delivered doses evaluated from the conversion coefficient of each project and Hp(3) doses evaluated from the nanoDots were found to be not exceeding -11.48 %, -8.85 % and -8.85 % for ORAMED, PTB and CEA Saclay project, respectively.

Coupled-Flow Simulation of HP-LP Turbines Has Resulted in Significant Fuel Savings

Science.gov (United States)

Veres, Joseph P.

2001-01-01

Our objective was to create a high-fidelity Navier-Stokes computer simulation of the flow through the turbines of a modern high-bypass-ratio turbofan engine. The simulation would have to capture the aerodynamic interactions between closely coupled high- and low-pressure turbines. A computer simulation of the flow in the GE90 turbofan engine's high-pressure (HP) and low-pressure (LP) turbines was created at GE Aircraft Engines under contract with the NASA Glenn Research Center. The three-dimensional steady-state computer simulation was performed using Glenn's average-passage approach named APNASA. The areas upstream and downstream of each blade row mutually interact with each other during engine operation. The embedded blade row operating conditions are modeled since the average passage equations in APNASA actively include the effects of the adjacent blade rows. The turbine airfoils, platforms, and casing are actively cooled by compressor bleed air. Hot gas leaks around the tips of rotors through labyrinth seals. The flow exiting the high work HP turbines is partially transonic and, therefore, has a strong shock system in the transition region. The simulation was done using 121 processors of a Silicon Graphics Origin 2000 (NAS 02K) cluster at the NASA Ames Research Center, with a parallel efficiency of 87 percent in 15 hr. The typical average-passage analysis mesh size per blade row was 280 by 45 by 55, or approx.700,000 grid points. The total number of blade rows was 18 for a combined HP and LP turbine system including the struts in the transition duct and exit guide vane, which contain 12.6 million grid points. Design cycle turnaround time requirements ran typically from 24 to 48 hr of wall clock time. The number of iterations for convergence was 10,000 at 8.03x10(exp -5) sec/iteration/grid point (NAS O2K). Parallel processing by up to 40 processors is required to meet the design cycle time constraints. This is the first-ever flow simulation of an HP and LP

FIST - a suite of X-ray powder crystallography programs for use with a HP-65 calculator

International Nuclear Information System (INIS)

Ferguson, I.F.; Turek, M.

1977-12-01

Programs for X-ray powder crystallography are defined for use with a Hewlett Packard HP-65 (programmable) pocket calculator. These include the prediction of all Bragg reflections for defined P-, F-, I-cubic, tetragonal, hexagonal and orthorhombic cells; the calculation of the position of a specific Bragg reflection from defined unit cells with all symmetries except triclinic; interconversion of theta, 2theta, sin 2 theta and d, as well as the calculation of the Nelson-Riley function; the computation of crystal densities; the interconversion of rhombohedral and hexagonal unit cells, lsub(c) determinations for graphite, the calculation of a and c for boron carbide; and Miller index transformations between various unit cells. (author)

Streptococcus oligofermentans Inhibits Streptococcus mutans in Biofilms at Both Neutral pH and Cariogenic Conditions.

Science.gov (United States)

Bao, Xudong; de Soet, Johannes Jacob; Tong, Huichun; Gao, Xuejun; He, Libang; van Loveren, Cor; Deng, Dong Mei

2015-01-01

Homeostasis of oral microbiota can be maintained through microbial interactions. Previous studies showed that Streptococcus oligofermentans, a non-mutans streptococci frequently isolated from caries-free subjects, inhibited the cariogenic Streptococcus mutans by the production of hydrogen peroxide (HP). Since pH is a critical factor in caries formation, we aimed to study the influence of pH on the competition between S. oligofermentans and S. mutans in biofilms. To this end, S. mutans and S. oligofermentans were inoculated alone or mixed at 1:1 ratio in buffered biofilm medium in a 96-well active attachment model. The single- and dual-species biofilms were grown under either constantly neutral pH or pH-cycling conditions. The latter includes two cycles of 8 h neutral pH and 16 h pH 5.5, used to mimic cariogenic condition. The 48 h biofilms were analysed for the viable cell counts, lactate and HP production. The last two measurements were carried out after incubating the 48 h biofilms in buffers supplemented with 1% glucose (pH 7.0) for 4 h. The results showed that S. oligofermentans inhibited the growth of S. mutans in dual-species biofilms under both tested pH conditions. The lactic acid production of dual-species biofilms was significantly lower than that of single-species S. mutans biofilms. Moreover, dual-species and single-species S. oligofermentans biofilms grown under pH-cycling conditions (with a 16 h low pH period) produced a significantly higher amount of HP than those grown under constantly neutral pH. In conclusion, S. oligofermentans inhibited S. mutans in biofilms not only under neutral pH, but also under pH-cycling conditions, likely through HP production. S. oligofermentans may be a compelling probiotic candidate against caries.

Arbitrary-Level Hanging Nodes and Automatic Adaptivity in the hp-FEM

Czech Academy of Sciences Publication Activity Database

Šolín, Pavel; Červený, Jakub; Doležel, Ivo

2008-01-01

Roč. 77, č. 1 (2008), s. 117-132 ISSN 0378-4754 R&D Projects: GA ČR(CZ) GA102/07/0496; GA ČR GA102/05/0629; GA AV ČR IAA100760702 Institutional research plan: CEZ:AV0Z20570509 Keywords : constrained approximation * hanging nodes * hp-FEM Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.930, year: 2008

Photolysis of polychlorinated dibenzo-p-dioxins and dibenzofurans dissolved in vegetable oils: influence of oil quality

International Nuclear Information System (INIS)

Isosaari, Pirjo; Laine, Olli; Tuhkanen, Tuula; Vartiainen, Terttu

2005-01-01

Sunlight or ultraviolet light irradiation of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in the presence of vegetable oil offers a potential method for the cleanup of contaminated soil. In this study, the effects of different types of vegetable oils on the photochemical degradation of 1,2,3,4,6,7,8-heptachlorodibenzofuran and heptachlorodibenzo-p-dioxin (1,2,3,4,6,7,8-HpCDF/HpCDD) were investigated in the laboratory. Using a blacklight lamp as a source of ultraviolet light, 93-100% of 1,2,3,4,6,7,8-HpCDF degraded in 60 min in rapeseed oil, extra virgin olive oil and olive oil. Less degradation occurred in palm oil (59%), toluene (39%) and hexane (20%). The better degradation in vegetable oils in comparison with organic solvents was attributed to the photooxidation of lipids producing hydrogen for PCDD/F dechlorination. In addition to the hydrogen donor capacity, permeability of ultraviolet light was involved in the differences between vegetable oils. α-Tocopherol and chlorophyll did not influence the performance of oil at concentrations normally present in vegetable oils, whereas β-carotene had an inhibitory effect on the degradation of 1,2,3,4,6,7,8-HpCDF. Up to 28% of the degradation products of 1,2,3,4,6,7,8-HpCDF were formed via the dechlorination pathway. Products included both toxic (2,3,7,8-chlorinated) and non-toxic PCDD/Fs, the toxic PCDD/Fs being more stable. Irradiation of 1,2,3,4,6,7,8-HpCDD yielded only non-toxic dechlorination products. Polychlorinated hydroxybiphenyls (OH-PCBs), polychlorinated dihydroxybiphenyls (DOH-PCBs) and polychlorinated hydroxydiphenylethers (OH-PCDEs) containing one to seven chlorine atoms were not detected in irradiated HpCDF/HpCDD samples

Photolysis of polychlorinated dibenzo-p-dioxins and dibenzofurans dissolved in vegetable oils: influence of oil quality

Energy Technology Data Exchange (ETDEWEB)

Isosaari, Pirjo [National Public Health Institute, Department of Environmental Health, P.O. Box 95, FI-70701 Kuopio (Finland)]. E-mail: pirjo.isosaari@ktl.fi; Laine, Olli [National Public Health Institute, Department of Environmental Health, P.O. Box 95, FI-70701 Kuopio (Finland); Tuhkanen, Tuula [Tampere University of Technology, Environmental Engineering and Biotechnology, P.O. Box 541, FI-33101 Tampere (Finland); Vartiainen, Terttu [National Public Health Institute, Department of Environmental Health, P.O. Box 95, FI-70701 Kuopio (Finland); University of Kuopio, Department of Environmental Sciences, P.O. Box 1627, Kuopio, FI-70211 Kuopio (Finland)

2005-03-20

Sunlight or ultraviolet light irradiation of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in the presence of vegetable oil offers a potential method for the cleanup of contaminated soil. In this study, the effects of different types of vegetable oils on the photochemical degradation of 1,2,3,4,6,7,8-heptachlorodibenzofuran and heptachlorodibenzo-p-dioxin (1,2,3,4,6,7,8-HpCDF/HpCDD) were investigated in the laboratory. Using a blacklight lamp as a source of ultraviolet light, 93-100% of 1,2,3,4,6,7,8-HpCDF degraded in 60 min in rapeseed oil, extra virgin olive oil and olive oil. Less degradation occurred in palm oil (59%), toluene (39%) and hexane (20%). The better degradation in vegetable oils in comparison with organic solvents was attributed to the photooxidation of lipids producing hydrogen for PCDD/F dechlorination. In addition to the hydrogen donor capacity, permeability of ultraviolet light was involved in the differences between vegetable oils. {alpha}-Tocopherol and chlorophyll did not influence the performance of oil at concentrations normally present in vegetable oils, whereas {beta}-carotene had an inhibitory effect on the degradation of 1,2,3,4,6,7,8-HpCDF. Up to 28% of the degradation products of 1,2,3,4,6,7,8-HpCDF were formed via the dechlorination pathway. Products included both toxic (2,3,7,8-chlorinated) and non-toxic PCDD/Fs, the toxic PCDD/Fs being more stable. Irradiation of 1,2,3,4,6,7,8-HpCDD yielded only non-toxic dechlorination products. Polychlorinated hydroxybiphenyls (OH-PCBs), polychlorinated dihydroxybiphenyls (DOH-PCBs) and polychlorinated hydroxydiphenylethers (OH-PCDEs) containing one to seven chlorine atoms were not detected in irradiated HpCDF/HpCDD samples.

One step ahead of technology Cern and HP - an ideal team project

CERN Multimedia

Garvey, Kelsey

2009-01-01

"In 2004, the It team at Cern recognized the comprehensiveness and future of the LHC Computing Grid Project and realized that the IT infrastructures were not sufficient enough to manage the data. Cern therefore partnered with HP ProCurve the following year to minimize the day-to-day workload and improve infrastructure for data output" (1 page)

Vibration and Stability of 3000-hp, Titanium Chemical Process Blower

Directory of Open Access Journals (Sweden)

Les Gutzwiller

2003-01-01

Full Text Available This 74-in-diameter blower had an overhung rotor design of titanium construction, operating at 50 pounds per square inch gauge in a critical chemical plant process. The shaft was supported by oil-film bearings and was directdriven by a 3000-hp electric motor through a metal disk type of coupling. The operating speed was 1780 rpm. The blower shaft and motor shaft motion was monitored by Bently Nevada proximity probes and a Model 3100 monitoring system.

Ab initio study of the compound-energy modeling of multisublattice structures: The (hP6) Ni2In-type intermetallics of the Ni–In–Sn system

International Nuclear Information System (INIS)

Ramos de Debiaggi, S.; González Lemus, N.V.; Deluque Toro, C.; Fernández Guillermet, A.

2015-01-01

Highlights: • A DFT study of the compounds involved in CALPHAD modeling of the Ni–In–Sn (hP6) phase. • Several three-sublattice compounds of Ni, In, Sn and vacancies are studied ab initio. • Structural, cohesive and thermodynamic properties and the electronic DOS are reported. • Trends in calculated properties are correlated with changes in electronic structure. • A picture of the chemical bonding trends for these s-p/d type compounds is discussed. - Abstract: The thermodynamic modeling of non-stoichiometric, multisublattice intermetallic phases using the Compound-Energy Formalism (CEF) involves the determination of parameters representing the Gibbs energy (G m ) of binary compounds, the so-called “end-member compounds” (EMCs), which are often metastable or hypothetical. In current CALPHAD (i.e., “Calculation of Phase Diagrams”) work, these quantities are treated as free parameters to be determined by searching for the best fit to the available information in the optimization procedure. The general purpose of this paper is to propose a theoretical approach to the study of the EMCs which makes use of density-functional-theory (DFT) ab initio calculations. The present method is applied to the EMCs involved in the CEF modeling of the non-stoichiometric (hP6) Ni 2 In-structure type phase of the Ni–In and Ni–In–Sn systems using the three-sublattice models (Ni) 1 (Ni,Va) 1 (In,Ni) 1 and (Ni,Va) 1 (Ni,Va) 1 (In,Ni,Sn) 1 , respectively. By means of systematic ab initio projected augmented waves (PAW) calculations using the VASP code we study the EMCs involved in the CEF formulations of the G m for this phase in the binary and the ternary systems. Specifically, we study the twelve EMCs corresponding to the following sublattice occupations: (Ni) 1 (Ni) 1 (In) 1 , which is usually described as Ni:Ni:In (i.e., a compound with formula “Ni 2 In”), Ni:Ni:Ni (i.e., “Ni 3 ”), Ni:Ni:Sn (“Ni 2 Sn”), Ni:Va:In (i.e., “NiIn”), Ni:Va:Ni (i

PENINGKATAN CD8 DAN IFN-γ PADA HYPERSENSITIVITY PNEUMONITIS (HP AKIBAT PAJANAN DEBU PENGGILINGAN PADI

Directory of Open Access Journals (Sweden)

Isa Ma'rufi

2017-10-01

Full Text Available Abstrak     Masalah klinis kesehatan kerja pada pekerja penggilingan padi saat ini adalah penyakit saluran pernafasan. Penelitian ini bertujuan untuk menganalisis peningkatan CD8 dan IFN-γ pada Pneumonitis Hipersensitif (HP akibat pajanan debu penggilingan padi pada mencit  (Musmusculus BALB/C. Penelitian yang dilakukan adalah penelitian eksperimental laboratoris dengan hewan coba mencit (Musmusculus BALB/C,  rancangan penelitian yang digunakan adalah Randomized the post test only control group design. Mencit  (Musmusculus BALB/C dipajan dengan debu penggilingan padi selama empat jam/hari selama tiga puluh hari dengan konsentrasi 0.50 mg/m3, 0.75 mg/m3, 1.00 mg/m3. Variabel bebas adalah debu penggilingan padi, sedangkan variabel tergantung adalah   CD8 dan IFN-γ, serta gambaran histopatologis paru mencit, sedangkan variabel kendali adalah strain, berat badan, dan umur. Hasil penelitian menunjukkan bahwa terjadi peningkatan kadar CD8 antara kontrol dengan perlakuan, dan secara statistik  ada perbedaan yang bermakna. Kemudian terjadi peningkatan IFN-γ dan secara statistik ada perbedaan yang bermakna antara kontrol dan perlakuan pada mencit  (Musmusculus BALB/C. Hasil penelitian dapat digunakan sebagai dasar upaya pencegahan dan pemberian terapi penyakit HP lebih dini sehingga dapat meningkatkan kualitas paru.   Kata Kunci: CD8, IFN-γ,  debu penggilingan padi     Abtract   The purpose of this research was to analyze increase of CD8  and IFN-γ of Hypersensitivity Pneumonitis (HP as a result from the exposure of dust from paddy milling on mice (MusmusculusBALB/C. The research done was a laboratory experimental research with mice (MusMusculus as experimental animal.  The research design used was the post only control group design using mice (MusMusculus Balb/c as experimental animal.  Mice (Musmusculus Balb/c were exposed to dust from paddy milling for four (4 hours/day and it was done for thirty (30 days with the exposed

Developing Consensus on the CompHP Professional Standards for Health Promotion in Europe

Science.gov (United States)

Speller, Viv; Parish, Richard; Davison, Heather; Zilnyk, Anna

2012-01-01

Building on the CompHP Core Competencies for health promotion the Professional Standards for Health Promotion have been developed and consulted on across Europe. The standards were formulated to fit within the complexity of professional, occupational and educational standards frameworks in Europe as learning outcome standards with performance…

Holomorphic extension of generalizations of Hp functions

Directory of Open Access Journals (Sweden)

Richard D. Carmichael

1985-01-01

Full Text Available In recent analysis we have defined and studied holomorphic functions in tubes in ℂn which generalize the Hardy Hp functions in tubes. In this paper we consider functions f(z, z=x+iy, which are holomorphic in the tube TC=ℝn+iC, where C is the finite union of open convex cones Cj, j=1,…,m, and which satisfy the norm growth of our new functions. We prove a holomorphic extension theorem in which f(z, z ϵ TC, is shown to be extendable to a function which is holomorphic in T0(C=ℝn+i0(C, where 0(C is the convex hull of C, if the distributional boundary values in 𝒮′ of f(z from each connected component TCj of TC are equal.

Measurement of personal equivalent dose Hp(3) during procedures involving in positron emission tomography; Medida del equivalente de dosis personal en cristalino Hp(3) durante los procedimientos asociados a la tomografía por emisión de positrones

Energy Technology Data Exchange (ETDEWEB)

Guiu Souto, J.; Sánchez García, M.; Vázquez, R.; Pardo Montero, J.; Pombar Cameán, M.

2017-11-01

The aim of the present study consists to determinate the personal equivalent dose Hp(3) at eye lens during the procedures involved in positron emission tomography. We have monitored the nurse's staff which is responsible of handling and dispensing 18F. We characterize the relevant stages as the preparation, the transport and the administration of monodose syringes in terms of occupational dose. We observed that the preparation presents a major contribution to the occupational doses than the other stages. In addition, during those stage there is a correlation between the dose increments and handling activity. To establish the eye lens dose we use the following dosimetry set: a direct readout solid state dosimeter for general purposes calibrated at Hp(10) and a set of thermolumiscent dosimeters specific for 18F calibrated at Hp(3). Finally, for the dose intercomparison between above dosimeters we obtain a correction factor by Monte Carlo. The eye lens dose, with a value of 2.8 ± 0.4 mSv/yr, results more critical than the dose obtained in other locations, as extremities. [Spanish] El propósito del presente trabajo consiste en la determinación del equivalente de dosis personal Hp(3) en cristalino durante los procedimientos asociados a la tomografía por emisión de positrones. Se ha monitorizado al personal de enfermería que realiza las tareas de manipulación y dispensación del 18F. Se ha realizado un estudio caracterizando la dosis en las etapas más relevantes del procedimiento: la preparación manual de las jeringuillas, el transporte de las mismas y su posterior administración al paciente. Se observó que la preparación es la etapa que más contribuye a la dosis ocupacional, además durante dicha etapa se observa una clara correlación entre el incremento de dosis y la actividad manipulada. Para determinar la dosis en cristalino se ha utilizado el siguiente equipo dosimétrico: un detector de estado sólido de lectura directa de propósito general

The Spectral/hp-Finite Element Method for Partial Differential Equations

DEFF Research Database (Denmark)

Engsig-Karup, Allan Peter

2009-01-01

dimensions. In the course the chosen programming environment is Matlab, however, this is by no means a necessary requirement. The mathematical level needed to grasp the details of this set of notes requires an elementary background in mathematical analysis and linear algebra. Each chapter is supplemented......This set of lecture notes provides an elementary introduction to both the classical Finite Element Method (FEM) and the extended Spectral/$hp$-Finite Element Method for solving Partial Differential Equations (PDEs). Many problems in science and engineering can be formulated mathematically...

BRAND VALUE OF INNOVATIVE COMPANIES – A THEORETICAL STUDY

Directory of Open Access Journals (Sweden)

José Antonio Paganotti

2013-12-01

Full Text Available The objective of this paper is to present a trajectory of performance of high-tech companies, considered innovative. The paper presents the reasons for success, stagnation, decline and fall of a group of companies chosen due to growth or decline. This is a descriptive and bibliographical research. To this purpose, we selected fifteen of the largest companies in Information Technology and Telecommunications, according to ranking prepared by Interbrand and BrandZ agencies. It is observed that companies have implemented strategies geared to innovation, resulting in high investment in R & D, spent in the past two decades. The surveys allowed us to associate trajectory and changes in brand value to business strategies and to market choices in the last twenty years, such as the acquisition of Compaq by HP and EDS, the recovery trend of Apple, competition between Apple and Nokia in market value, and the stability of the IBM.

UM ESTUDO TEÓRICO SOBRE O VALOR DA MARCA DE EMPRESAS CONSIDERADAS INOVADORAS.

Directory of Open Access Journals (Sweden)

José Antonio Paganotti

2013-07-01

Full Text Available The objective of this paper is to present a trajectory of performance of high-tech companies, considered innovative. The paper presents the reasons for success, stagnation, decline and fall of a group of companies chosen due to growth or decline. This is a descriptive and bibliographical research. To this purpose, we selected fifteen of the largest companies in Information Technology and Telecommunications, according to ranking prepared by Interbrand and BrandZ agencies. It is observed that companies have implemented strategies geared to innovation, resulting in high investment in R & D, spent in the past two decades. The surveys allowed us to associate trajectory and changes in brand value to business strategies and to market choices in the last twenty years, such as the acquisition of Compaq by HP and EDS, the recovery trend of Apple, competition between Apple and Nokia in market value, and the stability of the IBM.

Helicobacter pylori protein HP0986 (TieA) interacts with mouse TNFR1 and triggers proinflammatory and proapoptotic signaling pathways in cultured macrophage cells (RAW 264.7).

Science.gov (United States)

Ansari, Suhail A; Devi, Savita; Tenguria, Shivendra; Kumar, Ashutosh; Ahmed, Niyaz

2014-08-01

HP0986 protein of Helicobacter pylori has been shown to trigger induction of proinflammatory cytokines (IL-8 and TNF-α) through the activation of NF-κB and also to induce Fas mediated apoptosis of human macrophage cells (THP-1). In this study, we unravel mechanistic details of the biological effects of this protein in a murine macrophage environment. Up regulation of MCP-1 and TNF-α in HP0986-induced RAW 264.7 cells occurred subsequent to the activation and translocation of NF-κB to the cell nucleus. Further, HP0986 induced apoptosis of RAW 264.7 cells through Fas activation and this was in agreement with previous observations made with THP-1 cells. Our studies indicated activation of TNFR1 through interaction with HP0986 and this elicited the aforementioned responses independent of TLR2, TLR4 or TNFR2. We found that mouse TNFR1 activation by HP0986 facilitates formation of a complex comprising of TNFR1, TRADD and TRAF2, and this occurs upstream of NF-κB activation. Furthermore, FADD also forms a second complex, at a later stage, together with TNFR1 and TRADD, resulting in caspase-8 activation and thereby the apoptosis of RAW 264.7 cells. In summary, our observations reveal finer details of the functional activity of HP0986 protein in relation to its behavior in a murine macrophage cell environment. These findings reconfirm the proinflammatory and apoptotic role of HP0986 signifying it to be an important trigger of innate responses. These observations form much needed baseline data entailing future in vivo studies of the functions of HP0986 in a murine model. Copyright © 2014 Elsevier Ltd. All rights reserved.

Critical Success Factors in Crafting Strategic Architecture for E-Learning at HP University

Science.gov (United States)

Sharma, Kunal; Pandit, Pallvi; Pandit, Parul

2011-01-01

Purpose: The purpose of this paper is to outline the critical success factors for crafting a strategic architecture for e-learning at HP University. Design/methodology/approach: A descriptive survey type of research design was used. An empirical study was conducted on students enrolled with the International Centre for Distance and Open Learning…

The variable phenotype of the p.A16V mutation of cationic trypsinogen (PRSS1) in pancreatitis families

DEFF Research Database (Denmark)

Grocock, Christopher J; Rebours, Vinciane; Delhaye, Myriam

2010-01-01

Pancreatitis (HP); idiopathic disease; or pancreatitis in a single generation. HP was defined as 2 cases in 2 generations. MAIN OUTCOME MEASURES: Onset of painful episodes of pancreatitis, death from pancreatic cancer, diagnosis of diabetes mellitus and exocrine pancreatic failure. RESULTS: Ten families with p...... or Mann-Whitney-U tests. CONCLUSIONS: Penetrance of p.A16V is highly variable and family dependent, suggesting it contributes to a multigenic inheritance of a predisposition to pancreatitis....

Recent advances with quiescent power supply current (I(sub DDQ)) testing at Sandia using the HP82000

Science.gov (United States)

Righter, A. W.; Leong, D. J.; Cox, L. B.

Last year at the HP82000 Users Group Meeting, Sandia National Laboratories gave a presentation on I(sub DDQ) testing. This year, some advances are presented on this testing including DUT board fixturing, external DC PMU measurement, and automatic IDD-All circuit calibration. Implementation is examined more than theory, with results presented from Sandia tests. After a brief summary I(sub DDQ) theory and testing concepts, how the break (hold state) vector and data formatting present a test vector generation concern for the HP82000 is described. Fixturing of the DUT board for both types of I(sub DDQ) measurement is then discussed, along with how the continuity test and test vector generation must be taken into account. Results of a test including continuity, IDD-All and I(sub DDQ) Value measurements is shown. Next, measurement of low current using an external PMU is discussed, including noise considerations, implementation and some test results showing nA-range measurements. A method is presented for automatic calibration of the IDD-All analog comparator circuit using RM BASIC on the HP82000, with implementation and measurement results. Finally, future directions for research in this area is explored.

VLBA DETERMINATION OF THE DISTANCE TO NEARBY STAR-FORMING REGIONS. III. HP TAU/G2 AND THE THREE-DIMENSIONAL STRUCTURE OF TAURUS

International Nuclear Information System (INIS)

Torres, Rosa M.; Loinard, Laurent; Rodriguez, Luis F.; Mioduszewski, Amy J.

2009-01-01

Using multiepoch Very Long Baseline Array (VLBA) observations, we have measured the trigonometric parallax of the weak-line T Tauri star HP Tau/G2 in Taurus. The best fit yields a distance of 161.2 ± 0.9 pc, suggesting that the eastern portion of Taurus (where HP Tau/G2 is located) corresponds to the far side of the complex. Previous VLBA observations have shown that T Tau, to the south of the complex, is at an intermediate distance of about 147 pc, whereas the region around L1495 corresponds to the near side at roughly 130 pc. Our observations of only four sources are still too coarse to enable a reliable determination of the three-dimensional structure of the entire Taurus star-forming complex. They do demonstrate, however, that VLBA observations of multiple sources in a given star-forming region have the potential not only to provide a very accurate estimate of its mean distance, but also to reveal its internal structure. The proper motion measurements obtained simultaneously with the parallax allowed us to study the kinematics of the young stars in Taurus. Combining the four observations available so far, we estimate the peculiar velocity of Taurus to be about 10.6 km s -1 almost completely in a direction parallel to the Galactic plane. Using our improved distance measurement, we have refined the determination of the position on the H-R diagram of HP Tau/G2, and of two other members of the HP Tau group (HP Tau itself and HP Tau/G3). Most pre-main-sequence evolutionary models predict significantly discrepant ages (by 5 Myr) for those three stars-expected to be coeval. Only in the models of Palla and Stahler do they fall on a single isochrone (at 3 Myr).

Proportionate Dwarfism in Mice Lacking Heterochromatin Protein 1 Binding Protein 3 (HP1BP3) Is Associated With Alterations in the Endocrine IGF-1 Pathway.

Science.gov (United States)

Garfinkel, Benjamin P; Arad, Shiri; Le, Phuong T; Bustin, Michael; Rosen, Clifford J; Gabet, Yankel; Orly, Joseph

2015-12-01

Heterochromatin protein 1 binding protein 3 (HP1BP3) is a recently described histone H1-related protein with roles in chromatin structure and transcriptional regulation. To explore the potential physiological role of HP1BP3, we have previously described an Hp1bp3(-/-) mouse model with reduced postnatal viability and growth. We now find that these mice are proportionate dwarfs, with reduction in body weight, body length, and organ weight. In addition to their small size, microcomputed tomography analysis showed that Hp1bp3(-/-) mice present a dramatic impairment of their bone development and structure. By 3 weeks of age, mice of both sexes have severely impaired cortical and trabecular bone, and these defects persist into adulthood and beyond. Primary cultures of both osteoblasts and osteoclasts from Hp1bp3(-/-) bone marrow and splenocytes, respectively, showed normal differentiation and function, strongly suggesting that the impaired bone accrual is due to noncell autonomous systemic cues in vivo. One major endocrine pathway regulating both body growth and bone acquisition is the IGF regulatory system, composed of IGF-1, the IGF receptors, and the IGF-binding proteins (IGFBPs). At 3 weeks of age, Hp1bp3(-/-) mice exhibited a 60% reduction in circulating IGF-1 and a 4-fold increase in the levels of IGFBP-1 and IGFBP-2. These alterations were reflected in similar changes in the hepatic transcripts of the Igf1, Igfbp1, and Igfbp2 genes. Collectively, these results suggest that HP1BP3 plays a key role in normal growth and bone development by regulating transcription of endocrine IGF-1 components.

Proportionate Dwarfism in Mice Lacking Heterochromatin Protein 1 Binding Protein 3 (HP1BP3) Is Associated With Alterations in the Endocrine IGF-1 Pathway

OpenAIRE

Garfinkel, Benjamin P.; Arad, Shiri; Le, Phuong T.; Bustin, Michael; Rosen, Clifford J.; Gabet, Yankel; Orly, Joseph

2015-01-01

Heterochromatin protein 1 binding protein 3 (HP1BP3) is a recently described histone H1-related protein with roles in chromatin structure and transcriptional regulation. To explore the potential physiological role of HP1BP3, we have previously described an Hp1bp3?/? mouse model with reduced postnatal viability and growth. We now find that these mice are proportionate dwarfs, with reduction in body weight, body length, and organ weight. In addition to their small size, microcomputed tomography...

Animation company "Fast Forwards" production with HP Utility Data Center; film built using Adaptive Enterprise framework enabled by shared, virtual resource

CERN Multimedia

2003-01-01

Hewlett Packard have produced a commercial-quality animated film using an experimental rendering service from HP Labs and running on an HP Utility Data Center (UDC). The project demonstrates how computing resources can be managed virtually and illustrates the value of utility computing, in which an end-user taps into a large pool of virtual resources, but pays only for what is used (1 page).

Ab initio study of the compound-energy modeling of multisublattice structures: The (hP6) Ni{sub 2}In-type intermetallics of the Ni–In–Sn system

Energy Technology Data Exchange (ETDEWEB)

Ramos de Debiaggi, S., E-mail: susana.ramos@fain.uncoma.edu.ar [Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires 1400, 8300 Neuquén (Argentina); Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas – CONICET-UNCo (Argentina); González Lemus, N.V. [Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires 1400, 8300 Neuquén (Argentina); Deluque Toro, C. [Grupo de Nuevos Materiales, Universidad de la Guajira, Riohacha (Colombia); Fernández Guillermet, A. [CONICET - Instituto Balseiro, Centro Atómico Bariloche, Avda. Bustillo 9500, 8400 Bariloche (Argentina)

2015-01-15

Highlights: • A DFT study of the compounds involved in CALPHAD modeling of the Ni–In–Sn (hP6) phase. • Several three-sublattice compounds of Ni, In, Sn and vacancies are studied ab initio. • Structural, cohesive and thermodynamic properties and the electronic DOS are reported. • Trends in calculated properties are correlated with changes in electronic structure. • A picture of the chemical bonding trends for these s-p/d type compounds is discussed. - Abstract: The thermodynamic modeling of non-stoichiometric, multisublattice intermetallic phases using the Compound-Energy Formalism (CEF) involves the determination of parameters representing the Gibbs energy (G{sub m}) of binary compounds, the so-called “end-member compounds” (EMCs), which are often metastable or hypothetical. In current CALPHAD (i.e., “Calculation of Phase Diagrams”) work, these quantities are treated as free parameters to be determined by searching for the best fit to the available information in the optimization procedure. The general purpose of this paper is to propose a theoretical approach to the study of the EMCs which makes use of density-functional-theory (DFT) ab initio calculations. The present method is applied to the EMCs involved in the CEF modeling of the non-stoichiometric (hP6) Ni{sub 2}In-structure type phase of the Ni–In and Ni–In–Sn systems using the three-sublattice models (Ni){sub 1}(Ni,Va){sub 1}(In,Ni){sub 1} and (Ni,Va){sub 1}(Ni,Va){sub 1}(In,Ni,Sn){sub 1}, respectively. By means of systematic ab initio projected augmented waves (PAW) calculations using the VASP code we study the EMCs involved in the CEF formulations of the G{sub m} for this phase in the binary and the ternary systems. Specifically, we study the twelve EMCs corresponding to the following sublattice occupations: (Ni){sub 1}(Ni){sub 1}(In){sub 1}, which is usually described as Ni:Ni:In (i.e., a compound with formula “Ni{sub 2}In”), Ni:Ni:Ni (i.e., “Ni{sub 3}”), Ni:Ni:Sn (�

Emergence of highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) in medium-scale swine farms in southeastern Cambodia.

Science.gov (United States)

Tornimbene, B; Frossard, J-P; Chhim, V; Sorn, S; Guitian, J; Drew, T W

2015-01-01

Since 2006, reports from China and Viet Nam have alerted of an emergent highly pathogenic variant of porcine reproductive and respiratory syndrome virus (HP-PRRSV) in that region. The frequent occurrence of outbreaks in these countries puts Cambodian pig farms at high risk of infection, but no study had been conducted to investigate the presence of HP-PRRS in Cambodian farms. We investigated the presence of HP-PRRS in medium-scale (semi-commercial) swine farms in the Cambodian southeastern region. Specifically, one province bordering Viet Nam (Takeo) was selected due to the concentration of most semi-commercial farms in that province. A cross-sectional study was carried out, between July and September 2010 to assess whether the prevalence of infection in these farms was indicative of recent spread of PPRSV and to identify risk factors for infection. The number of farms to be sampled was established using methods for Lot Quality Assurance Surveys (LQAS), in order to achieve a pre-established ability to discriminate between two different prevalence settings. The target population comprised all semi-commercial farms in Takeo province from which a random sample of 35 farms was selected. Selected farms were visited and questionnaires administered to gather information on farm characteristics and husbandry practices. Blood samples from individual pigs were collected in each of the study farms and tested for PRRSV, along with a number of other swine respiratory pathogens in order to investigate potential interactions. Our results showed that the virus was already present in Takeo semi-commercial pig population (LQAS herd prevalence ≥85%) at the time of sampling. The presence of sows in the farm and farm density were significantly associated (P<0.05) with the introduction and the presence of PRRS - but this was an unadjusted association as small sample size precluded multivariate analysis. Spatiotemporal description of the supposed pattern of infection revealed that the

Derivation of P-T paths from high-pressure metagranites - Examples from the Gran Paradiso Massif, western Alps

Science.gov (United States)

Massonne, Hans-Joachim

2015-06-01

Metamorphosed granites (SiO2 ≥ 70 wt.%) are, in fact, a common rock type in high pressure (HP) and ultrahigh pressure (UHP) terrains, but these rocks were rarely used to derive metamorphic P-T paths. To test the suitability of HP metagranites for such derivations, two metagranites from the Gran Paradiso Massif were studied applying elemental mapping of phengite and garnet and calculated P-T pseudosections contoured by various chemical and modal parameters. Both rocks contain phengite with maximum Si contents of about 3.42 Si per formula unit (pfu) and 3.55 Si pfu in cores, and accessory garnet which is compositionally zoned. Garnet core compositions are rich in grossular component (XCa up to 0.72). Only a rough P-T path could be derived with peak pressures below 2 GPa because, for instance, Si contents in phengite become geobarometrically insensitive at HP conditions, when biotite is not anymore stable. A test of the pseudosection approach to a metagranite from the North Qaidam UHP metamorphic belt resulted in an ambiguous finding. In fact, compositions of garnet and phengite in this rock are indicative of both UHP and specific HP conditions ( 1.3 GPa, 530 °C), but the latter conditions fit the entire mineralogical observations better.

Off-Line High-pH Reversed-Phase Fractionation for In-Depth Phosphoproteomics

DEFF Research Database (Denmark)

Batth, Tanveer S; Francavilla, Chiara; Olsen, Jesper V

2014-01-01

thousands of phosphorylation sites. However, in-depth phosphoproteomics often require off-line enrichment and fractionation techniques. In this study, we provide a detailed analysis of the physicochemical characteristics of phosphopeptides, which have been fractionated by off-line high-pH chromatography (Hp...... phosphorylated peptides over that with SCX. Further optimizations in the pooling and concatenation strategy increased the total number of multiphosphorylated peptides detected after HpH fractionation. In conclusion, we provide a basic framework and resource for performing in-depth phosphoproteome studies...

High pH reversed-phase chromatography as a superior fractionation scheme compared to off-gel isoelectric focusing for complex proteome analysis.

Science.gov (United States)

Stein, Derek R; Hu, Xiaojie; McCorrister, Stuart J; Westmacott, Garrett R; Plummer, Francis A; Ball, Terry B; Carpenter, Michael S

2013-10-01

MS/MS is the technology of choice for analyzing complex protein mixtures. However, due to the intrinsic complexity and dynamic range present in higher eukaryotic proteomes, prefractionation is an important step to maximize the number of proteins identified. Off-gel IEF (OG-IEF) and high pH RP (Hp-RP) column chromatography have both been successfully utilized as a first-dimension peptide separation technique in shotgun proteomic experiments. Here, a direct comparison of the two methodologies was performed on ex vivo peripheral blood mononuclear cell lysate. In 12-fraction replicate analysis, Hp-RP resulted in more peptides and proteins identified than OG-IEF fractionation. Distributions of peptide pIs and hydropathy did not reveal any appreciable bias in either technique. Resolution, defined here as the ability to limit a specific peptide to one particular fraction, was significantly better for Hp-RP. This leads to a more uniform distribution of total and unique peptides for Hp-RP across all fractions collected. These results suggest that fractionation by Hp-RP over OG-IEF is the better choice for typical complex proteome analysis. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

CESEC III code conversion from Apollo to HP9000

International Nuclear Information System (INIS)

Lee, Hae Cho

1996-01-01

CESEC code is a computer program used to analyze transient behaviour of reactor coolant systems of nuclear power plants. CESEC III is an extension of original CESEC code in order to apply wide range of accident analysis including ATWS model. Major parameters during the transients are calculated by CESEC. This report firstly describes detailed work carried out for installation of CESEC III on Apollo DN10000 and code validation results after installation. Secondly, A series of work is also described in relation to installation of CESECIII on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

CESEC III code conversion from Apollo to HP9000

Energy Technology Data Exchange (ETDEWEB)

Lee, Hae Cho [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

1996-01-01

CESEC code is a computer program used to analyze transient behaviour of reactor coolant systems of nuclear power plants. CESEC III is an extension of original CESEC code in order to apply wide range of accident analysis including ATWS model. Major parameters during the transients are calculated by CESEC. This report firstly describes detailed work carried out for installation of CESEC III on Apollo DN10000 and code validation results after installation. Secondly, A series of work is also described in relation to installation of CESECIII on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new.

HP Memristor mathematical model for periodic signals and DC

KAUST Repository

Radwan, Ahmed G.

2012-07-28

In this paper mathematical models of the HP Memristor for DC and periodic signal inputs are provided. The need for a rigid model for the Memristor using conventional current and voltage quantities is essential for the development of many promising Memristors\\' applications. Unlike the previous works, which focuses on the sinusoidal input waveform, we derived rules for any periodic signals in general in terms of voltage and current. Square and triangle waveforms are studied explicitly, extending the formulas for any general square wave. The limiting conditions for saturation are also provided in case of either DC or periodic signals. The derived equations are compared to the SPICE model of the Memristor showing a perfect match.

Spectral/hp least-squares finite element formulation for the Navier-Stokes equations

International Nuclear Information System (INIS)

Pontaza, J.P.; Reddy, J.N.

2003-01-01

We consider the application of least-squares finite element models combined with spectral/hp methods for the numerical solution of viscous flow problems. The paper presents the formulation, validation, and application of a spectral/hp algorithm to the numerical solution of the Navier-Stokes equations governing two- and three-dimensional stationary incompressible and low-speed compressible flows. The Navier-Stokes equations are expressed as an equivalent set of first-order equations by introducing vorticity or velocity gradients as additional independent variables and the least-squares method is used to develop the finite element model. High-order element expansions are used to construct the discrete model. The discrete model thus obtained is linearized by Newton's method, resulting in a linear system of equations with a symmetric positive definite coefficient matrix that is solved in a fully coupled manner by a preconditioned conjugate gradient method. Spectral convergence of the L 2 least-squares functional and L 2 error norms is verified using smooth solutions to the two-dimensional stationary Poisson and incompressible Navier-Stokes equations. Numerical results for flow over a backward-facing step, steady flow past a circular cylinder, three-dimensional lid-driven cavity flow, and compressible buoyant flow inside a square enclosure are presented to demonstrate the predictive capability and robustness of the proposed formulation

Conversion of the COBRA-IV-I code from CDC CYBER to HP 9000/700 version

International Nuclear Information System (INIS)

Sohn, D. S.; Yoo, Y. J.; Nahm, K. Y.; Hwang, D. H.

1996-01-01

COBRA-IV-I is a multichannel analysis code for the thermal-hydraulic analysis of rod bundle nuclear fuel elements and cores based on the subchannel approach. The existing COBRA-IV-I code is the control data corporation (CDC) CYBER version, which has limitations on the computer core storage and gives some inconvenience to the user interface. To solve these problems, we have converted the COBRA-IV-I code form the CDC CYBER mainframe to an Hewlett Packard (HP) 9000/700-series workstation version, and have verified the converted code. as a result, we have found almost no difference between the two versions in their calculation results. Therefore we expect the HP 9000/700 version of the COBRA-IV-I code to be the basis for the future development of an improved multichannel analysis code under the more convenient user environment. (author). 3 tabs., 2 figs., 8 refs

A comparative study of stool antigen Immunocard STAT HpSA test with biopsy in diagnosis of H Pylori Infection

Directory of Open Access Journals (Sweden)

Munish Rastogi

2015-12-01

Full Text Available The present study was conducted on 78 cases in the Department of Medicine at G.S.V.M Medical College, Kanpur who reported with various gastrointestinal symptoms such as dyspepsia, heartburns, nausea, vomiting and loss of appetite. They all were advised for biopsy by the physician. Patients treated with antibiotics, bismuth or proton pump inhibitors within 4 weeks preceding the study were excluded. Invasive tests using mucosal biopsies including histology and rapid urease test (RUT were used to establish the gold standard in order to evaluate the accuracy of Immunocard STAT HpSA. Sensitivity, Specificity, Positive Predictive Value and Negative Predictive Value of Immunocard STAT HpSA as compared to gold standard were 95.5%, 81.8%, 96.9% and 75.0% respectively. It is thus concluded that the Immunocard STAT HpSA test has a diagnostic value comparable to the gold standard in detecting H Pylori. The sensitivity and specificity of the test is fair enough to be used as a test for screening purpose and also for diagnosis and treatment of H.Pylori infection in clinical practice

Search for higher twist in γp interactions

International Nuclear Information System (INIS)

Hoeger, K.C.

1989-01-01

A comparison of the experimental observations of the inclusive production of charged particles in γp interactions with 70 GeV - γ << 170 GeV and hp (h=π, K) interactions with E=80 GeV and 140 GeV is presented. (HSI)

Seven health physics calculator programs for the HP-41CV

International Nuclear Information System (INIS)

Rittmann, P.D.

1984-08-01

Several user-oriented programs for the Hewlett-Packard HP-41CV are explained. The first program builds, stores, alters, and ages a list of radionuclides. This program only handles single- and double-decay chains. The second program performs convenient conversions for the six nuclides of concern in plutonium handling. The conversions are between mass, activity, and weight percents of the isotopes. The source can be aged and/or neutron generation rates can be computed. The third program is a timekeeping program that improves the process of manually estimating and tracking personnel exposure during high dose rate tasks by replacing the pencil, paper, and stopwatch method. This program requires a time module. The remaining four programs deal with computations of time-integrated air concentrations at various distances from an airborne release. Building wake effects, source depletion by ground deposition, and sector averaging can all be included in the final printout of the X/Q - Hanford and X/Q - Pasquill programs. The shorter versions of these, H/Q and P/Q, compute centerline or sector-averaged values and include a subroutine to facilitate dose estimation by entering dose factors and quantities released. The horizontal and vertical dispersion parameters in the Pasquill-Gifford programs were modeled with simple, two-parameter functions that agreed very well with the usual textbook graphs. 8 references, 7 appendices

An hp-adaptive strategy for the solution of the exact kernel curved wire Pocklington equation

NARCIS (Netherlands)

D.J.P. Lahaye (Domenico); P.W. Hemker (Piet)

2007-01-01

textabstractIn this paper we introduce an adaptive method for the numerical solution of the Pocklington integro-differential equation with exact kernel for the current induced in a smoothly curved thin wire antenna. The hp-adaptive technique is based on the representation of the discrete solution,

Calculation of conversion coefficients Hp(3)/K air using the PENELOPE Monte Carlo code and comparison with MCNP calculation results

International Nuclear Information System (INIS)

Daures, J.; Gouriou, J.; Bordy, J.M.

2010-01-01

The authors report calculations performed using the MNCP and PENELOPE codes to determine the Hp(3)/K air conversion coefficient which allows the Hp(3) dose equivalent to be determined from the measured value of the kerma in the air. They report the definition of the phantom, a 20 cm diameter and 20 cm high cylinder which is considered as representative of a head. Calculations are performed for an energy range corresponding to interventional radiology or cardiology (20 keV-110 keV). Results obtained with both codes are compared

Silent decision: HP1 protein escorts heterochromatic RNAs to their destiny

Science.gov (United States)

Ren, Jie; Martienssen, Robert A

2012-01-01

EMBO J advance online publication 06072012; doi:10.1016/j.molcel.2012.05.009 Heterochromatin is classically perceived to be refractory to transcription because of its compact structure. However, Keller et al (2012) now demonstrated that heterochromatic transcripts can accumulate even when heterochromatin is normally packaged. By tracking down the fate of these heterochromatic RNAs, they revealed a new post-transcriptional mechanism of silencing in heterochromatin that involves the dynamic turnover of HP1Swi6 between its free, chromatin-bound and RNA-bound forms. The latter form escorts heterochromatic RNA to degradation. PMID:22705945

Trypanosoma brucei gambiense group 1 is distinguished by a unique amino acid substitution in the HpHb receptor implicated in human serum resistance.

Directory of Open Access Journals (Sweden)

Rebecca E Symula

Full Text Available Trypanosoma brucei rhodesiense (Tbr and T. b. gambiense (Tbg, causative agents of Human African Trypanosomiasis (sleeping sickness in Africa, have evolved alternative mechanisms of resisting the activity of trypanosome lytic factors (TLFs, components of innate immunity in human serum that protect against infection by other African trypanosomes. In Tbr, lytic activity is suppressed by the Tbr-specific serum-resistance associated (SRA protein. The mechanism in Tbg is less well understood but has been hypothesized to involve altered activity and expression of haptoglobin haemoglobin receptor (HpHbR. HpHbR has been shown to facilitate internalization of TLF-1 in T.b. brucei (Tbb, a member of the T. brucei species complex that is susceptible to human serum. By evaluating the genetic variability of HpHbR in a comprehensive geographical and taxonomic context, we show that a single substitution that replaces leucine with serine at position 210 is conserved in the most widespread form of Tbg (Tbg group 1 and not found in related taxa, which are either human serum susceptible (Tbb or known to resist lysis via an alternative mechanism (Tbr and Tbg group 2. We hypothesize that this single substitution contributes to reduced uptake of TLF and thus may play a key role in conferring serum resistance to Tbg group 1. In contrast, similarity in HpHbR sequence among isolates of Tbg group 2 and Tbb/Tbr provides further evidence that human serum resistance in Tbg group 2 is likely independent of HpHbR function.

Perspectives on Industrial Innovation from Agilent, HP, and Bell Labs

Science.gov (United States)

Hollenhorst, James

2014-03-01

Innovation is the life blood of technology companies. I will give perspectives gleaned from a career in research and development at Bell Labs, HP Labs, and Agilent Labs, from the point of view of an individual contributor and a manager. Physicists bring a unique set of skills to the corporate environment, including a desire to understand the fundamentals, a solid foundation in physical principles, expertise in applied mathematics, and most importantly, an attitude: namely, that hard problems can be solved by breaking them into manageable pieces. In my experience, hiring managers in industry seldom explicitly search for physicists, but they want people with those skills.

The effect of fast particles' irradiation on electrooptical properties of GaP LEDs

International Nuclear Information System (INIS)

Gontaruk, O.; Kovalenko, A.; Malyj, E.; Petrenko, I.; Pinkovska, M.; Polivtcev, L.; Tartachnyk, V.

2013-01-01

The electrooptical properties of industrial AL 102 GaP light-emitting diodes (LEDs) irradiated with fast particles have been studied. 2 MeV electrons and fast reactor neutrons were used and devices' microplsma emitting, current-voltage and capacitance-voltage characteristics were measured. It was shown that electron irradiation in the range (2-5)10 16 cm -2 leads to the drop of microplasma emitting intensity, decrease of capacity and reverse currents and increase of reverse bias. The annealing study of current-voltage characteristics after neutron irradiation of diodes confirms the assumption about prevailing radiation influence on diode base due to induce of deep levels. (authors)

Stability of Basalt plus Anhydrite plus Calcite at HP-HT: Implications for Venus, the Earth and Mars

Science.gov (United States)

Martin, A. M.; Righter, K.; Treiman, A. H.

2010-01-01

"Canali" observed at Venus surface by Magellan are evidence for very long melt flows, but their composition and origin remain uncertain. The hypothesis of water-rich flow is not reasonable regarding the temperature at Venus surface. The length of these channels could not be explained by a silicate melt composition but more likely, by a carbonate-sulfate melt which has a much lower viscosity (Kargel et al 1994). One hypothesis is that calcite CaCO3 and anhydrite CaSO4 which are alteration products of basalts melted during meteorite impacts. A famous example recorded on the Earth (Chicxulub) produced melt and gas rich in carbon and sulfur. Calcite and sulfate evaporites are also present on Mars surface, associated with basalts. An impact on these materials might release C- and S-rich melt or fluid. Another type of planetary phenomenon (affecting only the Earth) might provoke a high pressure destabilization of basalt+anhydrite+calcite. Very high contents of C and S are measured in some Earth s magmas, either dissolved or in the form of crystals (Luhr 2008). As shown by the high H content and high fO2 of primary igneous anhydrite-bearing lavas, the high S content in their source may be explained by subduction of an anhydrite-bearing oceanic crust, either directly (by melting followed by eruption) or indirectly (by release of S-rich melt or fluid that metasomatize the mantle) . Calcite is a major product of oceanic sedimentation and alteration of the crust. Therefore, sulfate- and calcite-rich material may be subducted to high pressures and high temperatures (HP-HT) and release S- and C-rich melts or fluids which could influence the composition of subduction zone lavas or gases. Both phenomena - meteorite impact and subduction - imply HP-HT conditions - although the P-T-time paths are different. Some HP experimental/theoretical studies have been performed on basalt/eclogite, calcite and anhydrite separately or on a combination of two. In this study we performed piston

Mua (HP0868) Is a Nickel-Binding Protein That Modulates Urease Activity in Helicobacter pylori

Science.gov (United States)

Benoit, Stéphane L.; Maier, Robert J.

2011-01-01

A novel mechanism aimed at controlling urease expression in Helicobacter pylori in the presence of ample nickel is described. Higher urease activities were observed in an hp0868 mutant (than in the wild type) in cells supplemented with nickel, suggesting that the HP0868 protein (herein named Mua for modulator of urease activity) represses urease activity when nickel concentrations are ample. The increase in urease activity in the Δmua mutant was linked to an increase in urease transcription and synthesis, as shown by quantitative real-time PCR, SDS-PAGE, and immunoblotting against UreAB. Increased urease synthesis was also detected in a Δmua ΔnikR double mutant strain. The Δmua mutant was more sensitive to nickel toxicity but more resistant to acid challenge than was the wild-type strain. Pure Mua protein binds 2 moles of Ni2+ per mole of dimer. Electrophoretic mobility shift assays did not reveal any binding of Mua to the ureA promoter or other selected promoters (nikR, arsRS, 5′ ureB-sRNAp). Previous yeast two-hybrid studies indicated that Mua and RpoD may interact; however, only a weak interaction was detected via cross-linking with pure components and this could not be verified by another approach. There was no significant difference in the intracellular nickel level between wild-type and mua mutant cells. Taken together, our results suggest the HP0868 gene product represses urease transcription when nickel levels are high through an as-yet-uncharacterized mechanism, thus counterbalancing the well-described NikR-mediated activation. PMID:21505055

Dynamic variation of histone H3 trimethyl Lys4 (H3K4me3) and heterochromatin protein 1 (HP1) with employment length in nickel smelting workers.

Science.gov (United States)

Zhao, Yanhong; Cheng, Ning; Dai, Min; Pu, Hongquan; Zheng, Tongzhang; Li, Haiyan; He, Jie; Bai, Yana

2017-07-01

To investigate the dynamic variation in H3K4me3 and HP1 with employment length in nickel smelting workers. Blood samples were collected from 140 nickel smelting workers and 140 age-matched office workers to test for H3K4me3, and HP1 levels. H3K4me3 was statistically significantly different (p exposure to nickel can induce oxidative damage, and increase H3K4me3 expression and inhibit HP1 expression.

A point kernel shielding code, PKN-HP, for high energy proton incident

Energy Technology Data Exchange (ETDEWEB)

Kotegawa, Hiroshi [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

1996-06-01

A point kernel integral technique code PKN-HP, and the related thick target neutron yield data have been developed to calculate neutron and secondary gamma-ray dose equivalents in ordinary concrete and iron shields for fully stopping length C, Cu and U-238 target neutrons produced by 100 MeV-10 GeV proton incident in a 3-dimensional geometry. The comparisons among calculation results of the present code and other calculation techniques, and measured values showed the usefulness of the code. (author)

Application of a Long Term Asset Management Strategy for HP Feedwater Heaters

International Nuclear Information System (INIS)

Won, Se Youl; Yun, Eun Sub; Park, Young Sheop

2008-01-01

As the commercial operating year of nuclear power plants is increased, it becomes imperative to develop integrated cost-effective asset management and to improve plans for degraded Structures, Systems, and Components (SSCs) in terms of safety and economical consideration. A long-term asset management (LTAM) strategy can improve the condition of nuclear plants, maximize their value, and optimize their operational life by maintaining their safety. This paper presents an optimized LTAM plan for HP feedwater heaters at a specific nuclear power plant

Enrichment of HP1a on Drosophila chromosome 4 genes creates an alternate chromatin structure critical for regulation in this heterochromatic domain.

Directory of Open Access Journals (Sweden)

Nicole C Riddle

2012-09-01

Full Text Available Chromatin environments differ greatly within a eukaryotic genome, depending on expression state, chromosomal location, and nuclear position. In genomic regions characterized by high repeat content and high gene density, chromatin structure must silence transposable elements but permit expression of embedded genes. We have investigated one such region, chromosome 4 of Drosophila melanogaster. Using chromatin-immunoprecipitation followed by microarray (ChIP-chip analysis, we examined enrichment patterns of 20 histone modifications and 25 chromosomal proteins in S2 and BG3 cells, as well as the changes in several marks resulting from mutations in key proteins. Active genes on chromosome 4 are distinct from those in euchromatin or pericentric heterochromatin: while there is a depletion of silencing marks at the transcription start sites (TSSs, HP1a and H3K9me3, but not H3K9me2, are enriched strongly over gene bodies. Intriguingly, genes on chromosome 4 are less frequently associated with paused polymerase. However, when the chromatin is altered by depleting HP1a or POF, the RNA pol II enrichment patterns of many chromosome 4 genes shift, showing a significant decrease over gene bodies but not at TSSs, accompanied by lower expression of those genes. Chromosome 4 genes have a low incidence of TRL/GAGA factor binding sites and a low T(m downstream of the TSS, characteristics that could contribute to a low incidence of RNA polymerase pausing. Our data also indicate that EGG and POF jointly regulate H3K9 methylation and promote HP1a binding over gene bodies, while HP1a targeting and H3K9 methylation are maintained at the repeats by an independent mechanism. The HP1a-enriched, POF-associated chromatin structure over the gene bodies may represent one type of adaptation for genes embedded in repetitive DNA.

Discrete maximum principle for Poisson equation with mixed boundary conditions solved by hp-FEM

Czech Academy of Sciences Publication Activity Database

Vejchodský, Tomáš; Šolín, P.

2009-01-01

Roč. 1, č. 2 (2009), s. 201-214 ISSN 2070-0733 R&D Projects: GA AV ČR IAA100760702; GA ČR(CZ) GA102/07/0496; GA ČR GA102/05/0629 Institutional research plan: CEZ:AV0Z10190503 Keywords : discrete maximum principle * hp-FEM * Poisson equation * mixed boundary conditions Subject RIV: BA - General Mathematics

Susceptibility of the Tomato Mutant High Pigment-2dg (hp-2dg) to Orobanche spp. Infection

NARCIS (Netherlands)

Lopez Raez, J.A.; Charnikhova, T.; Mulder, P.P.J.; Kohlen, W.; Bino, R.J.; Levin, I.; Bouwmeester, H.J.

2008-01-01

The consumption of natural products with potential health benefits has been continuously growing, and enhanced pigmentation is of major economic importance in fruits and vegetables. The tomato hp-2dg is an important mutant line that has been introgressed into commercial tomato cultivars marketed as

Comparative studies of the influence of cyclodextrins on the stability of the sunscreen agent, 2-ethylhexyl-p-methoxycinnamate.

Science.gov (United States)

Scalia, Santo; Casolari, Alberto; Iaconinoto, Antonietta; Simeoni, Silvia

2002-11-07

The effects of beta-cyclodextrin (beta-CD) and hydroxypropyl-beta-cyclodextrin (HP-beta-CD) on the base-catalyzed degradation and light-induced decomposition of the sunscreen agent, trans-2-ethylhexyl-p-methoxycinnamate (trans-EHMC) were investigated. Reversed-phase liquid chromatography was used to study the interaction between natural and modified cyclodextrins, added to the mobile phase, and the sunscreen. Among the available cyclodextrins (beta-CD, HP-beta-CD, hydroxypropyl-alpha-cyclodextrin and hydroxypropyl-gamma-cyclodextrin), only HP-beta-CD and beta-CD produced a significant decrease in the chromatographic retention of trans-EHMC. The complexation of the sunscreen agent with HP-beta-CD and beta-CD was confirmed by thermal analysis and nuclear magnetic resonance spectroscopy. beta-CD depressed the decomposition of trans-EHMC in alkaline solutions more effectively than HP-beta-CD. Moreover, the irradiation-induced degradation of the sunscreen agent in emulsion vehicles was reduced by complexation with beta-CD (the extent of degradation was 26.1% for the complex compared to 35.8% for free trans-EHMC) whereas HP-beta-CD had no significant effect. Therefore, the complex of beta-CD with trans-EHMC enhances the chemical- and photo-stability of the sunscreen agent. Moreover, it limits adverse interactions of the UV filter with other formulation ingredients.

Effect of hypoproteinemia (HP) upon FT4 and FT3 by kinetic radioligandassay (RLA)

International Nuclear Information System (INIS)

Bottger, I.G.; Schneck, H.J.

1985-01-01

HP, especially hypoalbuminemia (HA), has been associated with false low FT4 by RLA, predominantly of the analogue tracer type. Based upon previous more favourable findings in severe nonthyroidal illness (NTI), this study was designed to study this problem in more detail. Two groups of patients with severe NTI (polytrauma) were selected on the basis of their total serum protein (TSP) concentration and studied during intensive-care. Group I: N = 25, TSP 5.9 g/dl (6.0 - 7.7), A/G: 1.5, sera: N = 76. I and II: low-dose heparin (i.v. 5000 U/24 h), non-detectable in the periphery. The RLA kits used were: FT4/T4 and FT3/T3 (kinetic two-tube), and TBG, Corning Medical, rT3, Serono, TSH, Henning-Berlin, TSP/electrophoresis standard technique. The findings indicate: 1. Typical findings for follow-up of severe NTI; 2. No detection of a significant effect of HP/HA upon these RLAs for FT4 and FT3; and 3. The inter-group differences are most likely due to more severe NT1 in I (R/sub x/, A/G, rT3)

Quantification of bitumen particles in aerosol and soil samples using HP-GPC

DEFF Research Database (Denmark)

Fauser, Patrik; Tjell, Jens Christian; Mosbæk, Hans

2000-01-01

A method for identifying and quantifying bitumen particles, generated from the wear of roadway asphalts, in aerosol and soil samples has been developed. Bitumen is found to be the only contributor to airborne particles containing organic molecules with molecular weights larger than 2000 g pr. mol....... These are separated and identified using High Performance Gel Permeation Chromatography (HP-GPC) with fluorescence detection. As an additional detection method Infra Red spectrometry (IR) is employed for selected samples. The methods have been used on aerosol, soil and other samples....

A proposal for a test method for assessment of hazard property HP 12 ("Release of an acute toxic gas") in hazardous waste classification - Experience from 49 waste.

Science.gov (United States)

Hennebert, Pierre; Samaali, Ismahen; Molina, Pauline

2016-12-01

A stepwise method for assessment of the HP 12 is proposed and tested with 49 waste samples. The hazard property HP 12 is defined as "Release of an acute toxic gas": waste which releases acute toxic gases (Acute Tox. 1, 2 or 3) in contact with water or an acid. When a waste contains a substance assigned to one of the following supplemental hazards EUH029, EUH031 and EUH032, it shall be classified as hazardous by HP 12 according to test methods or guidelines (EC, 2014a, 2014b). When the substances with the cited hazard statement codes react with water or an acid, they can release HCl, Cl 2 , HF, HCN, PH 3 , H 2 S, SO 2 (and two other gases very unlikely to be emitted, hydrazoic acid HN 3 and selenium oxide SeO 2 - a solid with low vapor pressure). Hence, a method is proposed:For a set of 49 waste, water addition did not produce gas. Nearly all the solid waste produced a gas in contact with hydrochloric acid in 5 min in an automated calcimeter with a volume >0.1L of gas per kg of waste. Since a plateau of pressure is reached only for half of the samples in 5 min, 6 h trial with calorimetric bombs or glass flasks were done and confirmed the results. Identification of the gases by portable probes showed that most of the tested samples emit mainly CO 2 . Toxic gases are emitted by four waste: metallic dust from the aluminum industry (CO), two air pollution control residue of industrial waste incinerator (H 2 S) and a halogenated solvent (organic volatile(s) compound(s)). HF has not been measured in these trials started before the present definition of HP 12. According to the definition of HP 12, only the H 2 S emission of substances with hazard statement EUH031 is accounted for. In view of the calcium content of the two air pollution control residue, the presence of calcium sulphide (EUH031) can be assumed. These two waste are therefore classified potentially hazardous for HP 12, from a total of 49 waste. They are also classified as hazardous for other properties (HP 7

A proposal for a test method for assessment of hazard property HP 12 (“Release of an acute toxic gas”) in hazardous waste classification - Experience from 49 waste

OpenAIRE

Hennebert , Pierre; Samaali , Ismahen; Molina , Pauline

2016-01-01

International audience; A stepwise method for assessment of the HP 12 is proposed and tested with 49 waste samples. The hazard property HP 12 is defined as “Release of an acute toxic gas”: waste which releases acute toxic gases (Acute Tox. 1, 2 or 3) in contact with water or an acid. When a waste contains a substance assigned to one of the following supplemental hazards EUH029, EUH031 and EUH032, it shall be classified as hazardous by HP 12 according to test methods or guidelines (EC, 2014a, ...

Finite element analysis of the Girkmann problem using the modern hp-version and the classical h-version

KAUST Repository

Niemi, Antti; Babuška, Ivo M.; Pitkä ranta, Juhani; Demkowicz, Leszek F.

2011-01-01

elasticity theory and (2) by using a dimensionally reduced shell-ring model. In the first approach the problem is solved with a fully automatic hp-adaptive finite element solver whereas the classical h-version of the finite element method is used

Space and Time Adaptive Two-Mesh hp-Finite Element Method for Transient Microwave Heating Problems

Czech Academy of Sciences Publication Activity Database

Dubcová, Lenka; Šolín, Pavel; Červený, Jakub; Kůs, Pavel

1-2, č. 30 (2010), s. 23-40 ISSN 0272-6343 R&D Projects: GA ČR(CZ) GA102/07/0496; GA AV ČR IAA100760702 Institutional research plan: CEZ:AV0Z20570509 Keywords : hp-finite element method * microwave heating * edge elements Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.844, year: 2010

Maspin, p53, p63, and Ki-67 in epithelial lesions of the tongue: from hyperplasia through dysplasia to carcinoma.

Science.gov (United States)

Vered, Marilena; Allon, Irit; Dayan, Dan

2009-03-01

The pattern of changes in the expression of mammary serine protease inhibitor (maspin) tumor suppressor protein in tongue epithelial lesions [hyperplasia (HP), mild dysplasia (MD), moderate-to-severe dysplasia (MSD) and squamous cell carcinoma (SCC)] was investigated and correlated to the expression of maspin-regulating factors p53 and p63, and the proliferation marker Ki-67. Cases of HP (n = 16), MD (n = 12), MSD (n = 11), and SCC (n = 22) were immunostained for maspin, p53, p63, and Ki-67. Maspin expression was scored separately for the basal, middle, and upper thirds of the epithelial width, and as the total sum of all 'thirds' (maspin-total). p53, p63, and Ki-67 were immuno-morphometrically assessed for the entire epithelial width. Maspin expression was differential and progressive extending to higher epithelial layers as dysplastic changes aggravated and culminated in carcinoma. Strong expression was related to MSD in the middle third and to carcinoma in the upper third. It was frequently lost at the invasion front, where the tumor was less differentiated. The changes in mean scores of maspin-total in the different study groups were positively correlated to the mean scores of p63 (r = 0.5, P < 0.001), p53 (r = 0.4, P = 0.004), and Ki-67 (r = 0.5, P < 0.001). Strong expression of maspin in the middle third of the epithelium may be considered a diagnostic sign of mild-to-moderate dysplasia and an indication of carcinoma in the upper third. The correlations between maspin and controlling factors (e.g. p63 and p53) may be events with key roles in the development of tongue carcinoma.

Clinical efficacy and cost-effectiveness of HP-human FSH (Fostimon®) versus rFSH (Gonal-F®) in IVF-ICSI cycles: a meta-analysis.

Science.gov (United States)

Gerli, Sandro; Bini, Vittorio; Favilli, Alessandro; Di Renzo, Gian Carlo

2013-06-01

Clinical efficacy of human-derived follicle-stimulating hormone (FSH) versus recombinant FSH (rFSH) in IVF-ICSI cycles has long been compared, but no clear evidence of the superiority of a preparation over the other has been found. Human gonadotropins have been often grouped together, but a different glycosylation may be present in each preparation, therefore influencing the specific bioactivity. To exclude confounding factors, a meta-analysis and a cost-effectiveness analysis were designed to compare effectiveness and cost-effectiveness of a specific highly purified human FSH (HP-hFSH) (Fostimon®) versus rFSH (Gonal-F®) in IVF/ICSI cycles. Research methodology filters were applied in MEDLINE, Current Contents and Web of Science from 1980 to February 2012. Eight randomized trials met selection criteria. The meta-analysis showed no significant differences between rFSH and HP-hFSH treatment in live-birth rate (odds ratio [OR] 0.84, 95% confidence interval [CI] 0.63-1.11), clinical pregnancy rate (OR 0.85, 95% CI 0.68-1.07), number of oocytes retrieved, number of mature oocytes and days of stimulation. The cost-effectiveness ratio was € 7174 in the rFSH group and € 2056 in the HP-hFSH group. HP-hFSH is as effective as rFSH in ovarian stimulation for IVF-ICSI cycles, but the human preparation is more cost-effective.

Analysis and development of spatial hp-refinement methods for solving the neutron transport equation

International Nuclear Information System (INIS)

Fournier, D.

2011-01-01

The different neutronic parameters have to be calculated with a higher accuracy in order to design the 4. generation reactor cores. As memory storage and computation time are limited, adaptive methods are a solution to solve the neutron transport equation. The neutronic flux, solution of this equation, depends on the energy, angle and space. The different variables are successively discretized. The energy with a multigroup approach, considering the different quantities to be constant on each group, the angle by a collocation method called SN approximation. Once the energy and angle variable are discretized, a system of spatially-dependent hyperbolic equations has to be solved. Discontinuous finite elements are used to make possible the development of hp-refinement methods. Thus, the accuracy of the solution can be improved by spatial refinement (h-refinement), consisting into subdividing a cell into sub-cells, or by order refinement (p-refinement), by increasing the order of the polynomial basis. In this thesis, the properties of this methods are analyzed showing the importance of the regularity of the solution to choose the type of refinement. Thus, two error estimators are used to lead the refinement process. Whereas the first one requires high regularity hypothesis (analytical solution), the second one supposes only the minimal hypothesis required for the solution to exist. The comparison of both estimators is done on benchmarks where the analytic solution is known by the method of manufactured solutions. Thus, the behaviour of the solution as a regard of the regularity can be studied. It leads to a hp-refinement method using the two estimators. Then, a comparison is done with other existing methods on simplified but also realistic benchmarks coming from nuclear cores. These adaptive methods considerably reduces the computational cost and memory footprint. To further improve these two points, an approach with energy-dependent meshes is proposed. Actually, as the

Prediction of extracellular proteases of the human pathogen Helicobacter pylori reveals proteolytic activity of the Hp1018/19 protein HtrA.

Directory of Open Access Journals (Sweden)

Martin Löwer

Full Text Available Exported proteases of Helicobacter pylori (H. pylori are potentially involved in pathogen-associated disorders leading to gastric inflammation and neoplasia. By comprehensive sequence screening of the H. pylori proteome for predicted secreted proteases, we retrieved several candidate genes. We detected caseinolytic activities of several such proteases, which are released independently from the H. pylori type IV secretion system encoded by the cag pathogenicity island (cagPAI. Among these, we found the predicted serine protease HtrA (Hp1019, which was previously identified in the bacterial secretome of H. pylori. Importantly, we further found that the H. pylori genes hp1018 and hp1019 represent a single gene likely coding for an exported protein. Here, we directly verified proteolytic activity of HtrA in vitro and identified the HtrA protease in zymograms by mass spectrometry. Overexpressed and purified HtrA exhibited pronounced proteolytic activity, which is inactivated after mutation of Ser205 to alanine in the predicted active center of HtrA. These data demonstrate that H. pylori secretes HtrA as an active protease, which might represent a novel candidate target for therapeutic intervention strategies.

FLOOD 3 code conversion from Apollo to HP

Energy Technology Data Exchange (ETDEWEB)

Lee, Hae Cho [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

1996-01-01

FLOOD3 is a Fortran program used to analyze LOCA Reflood and Post Reflood transients for purpose of mechanistically generating containment sizing mass/energy source terms. The reflood time frame starts when safety injection water first enters the bottom of the core, following the initial LOCA blowdown phase, and the ends when liquid level in the core is high enough to quench the core. The post reflood phase starts at the end of reflood phase and lasts until the end of LOCA transient period. Longterm core cooling is initiated following post reflood phase. FLOOD3 code dose not contain separate capability for longterm cooling analysis. This report firstly describes detailed work carried out for installation of FLOOD3 on Apollo DN10000 and code validation results after installation. Secondly, A series of work is also describes in relation to installation of FLOOD3 on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new.

FLOOD 3 code conversion from Apollo to HP

International Nuclear Information System (INIS)

Lee, Hae Cho

1996-01-01

FLOOD3 is a Fortran program used to analyze LOCA Reflood and Post Reflood transients for purpose of mechanistically generating containment sizing mass/energy source terms. The reflood time frame starts when safety injection water first enters the bottom of the core, following the initial LOCA blowdown phase, and the ends when liquid level in the core is high enough to quench the core. The post reflood phase starts at the end of reflood phase and lasts until the end of LOCA transient period. Longterm core cooling is initiated following post reflood phase. FLOOD3 code dose not contain separate capability for longterm cooling analysis. This report firstly describes detailed work carried out for installation of FLOOD3 on Apollo DN10000 and code validation results after installation. Secondly, A series of work is also describes in relation to installation of FLOOD3 on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

Software quality assurance documentation for the release of NUFT 2.0 for HP platforms

International Nuclear Information System (INIS)

Fernandez, M.W.; Preckshot, G.G.; Johnson, G.L.

1998-01-01

This document is the Individual Software Plan (ISP) for version 2.0 of the Non-isothermal Unsaturated-saturated Flow and Transport (NUFT.) analysis computer program. This document addresses the applicable requirements of LLNL YMP procedure 033-YMP-QP 3.2, Section 4.2.1.1. The purpose of this ISP is to plan and organize the activities required to certify the NUFT code for quality affecting work involving problems that include cross drift analysis of the Yucca Mountain Repository facility. NUFT is software for application to the solution of a class of coupled mass and heat transport problems in porous geologic media including Yucca Mountain Repository Cross Drift Problem (YMRCDP- also known as the Enhanced Characterization of the Repository Block (ECRB)). Solution of this class of problems requires a suite of multiphase, multi-component models for numerical solution of non- isothermal flow and transport in porous media with applications to subsurface contaminant transport problems. NUFT is a suite of multiphase, multi-component models for numerical solution of non- isothermal flow and transport in porous media, with application to subsurface contaminant transport problems, and in particular, to the hydrology in and about the Yucca Mountain Repository Site. NUFI is acquired software, as defined by 033-YMP-QP 3.2, and a preliminary baseline of source code, electronic documentation, and paper documentation has been established as required by 033-YMP-QP 3.2, Section 4.1. NUFT runs on Sun Unix platforms, Solaris operating system version 5.5 and HP-UX with operating system version 10.20. The product to be qualified under this ISP is the version running on HP- UX. The HP version will be labeled Version 2.0h. The h is included to distinguish the HP version from possible future versions qualified for Sun or other platforms. The scope of the plans and procedures outlined in this ISP is limited to the effort required to qualify NUFT for the class of problems identified in

The effect of temperature and dot size on the spectral properties of colloidal InP/ZnS core-shell quantum dots.

Science.gov (United States)

Narayanaswamy, Arun; Feiner, L F; Meijerink, A; van der Zaag, P J

2009-09-22

Visual color changes between 300 and 510 K were observed in the photoluminescence (PL) of colloidal InP/ZnS core-shell nanocrystals. A subsequent study of PL spectra in the range 2-510 K and fitting the temperature dependent line shift and line width to theoretical models show that the dominant (dephasing) interaction is due to scattering by acoustic phonons of about 23 meV. Low temperature photoluminescence excitation measurements show that the excitonic band gap depends approximately inversely linearly on the quantum dot size d, which is distinctly weaker than the dependence predicted by current theories.

On the Realization of First-Order Current-Mode AP/HP Filter

Directory of Open Access Journals (Sweden)

W. Tangsrirat

2013-12-01

Full Text Available A compact circuit topology for the realization of the current-mode first-order allpass (AP and highpass (HP filters is described. The proposed circuit contains a minimum number of components, i.e., eight bipolar transistors and one grounded capacitor. The advantages of this circuit are the use of only grounded capacitor as a passive element, the electronic tunability of its parameters and its potential for low-voltage operation. Some simulation results are also reported, which demonstrate the effectiveness of the proposed circuit. Owing to the pole frequency of the filter circuit is normally dependent on temperature; a low-voltage translinear–based current source circuit for temperature compensation is also suggested.

Virus-cell fusion inhibitory activity of novel analogue peptides based on the HP (2-20) derived from N-terminus of Helicobacter pylori Ribosomal Protein L1.

Science.gov (United States)

Woo, Eun-Rhan; Lee, Dong Gun; Chang, Young-Su; Park, Yoonkyung; Hahm, Kyung-Soo

2002-12-01

HP (2-20) (AKKVFKRLEKLFSKIQNDK) is the antibacterial sequence derived from N-terminus of Helicobacter pylori Ribosomal Protein L1 (RPL1). It has a broad-spectrum microbicidal activity in vitro that is thought to be related to the membrane-disruptive properties of the peptide. Based on the putative membrane-targeted mode of action, we postulated that HP (2-20) might be possessed virus-cell fusion inhibitory activity. To develop the novel virus-cell fusion inhibitory peptides, several analogues with amino acid substitution were designed to increase or decrease only net hydrophobic region. In particular, substitution of Gln and Asp for hydrophobic amino acid, Trp at position 17 and 19 of HP (2-20) (Anal 3) caused a dramatic increase in virus-cell fusion inhibitory activity without hemolytic effect.

User manual of the multicompenent variably - saturated flow and transport model HP1

International Nuclear Information System (INIS)

Jacques, D.; Simunek, J.

2005-06-01

This report describes a new comprehensive simulation tool HP1 (HYDRUS1D-PHREEQC) that was obtained by coupling the HYDRUS-1D one-dimensional variably-saturated water flow and solute transport model with the PHREEQC geochemical code. The HP1 code incorporates modules simulating (1) transient water flow in variably-saturated media, (2) transport of multiple components, and (3) mixed equilibrium/kinetic geochemical reactions. The program numerically solves the Richards equation for variably-saturated water flow and advection-dispersion type equations for heat and solute transport. The flow equation incorporates a sink term to account for water uptake by plant roots. The heat transport equation considers transport due to conduction and convection with flowing water. The solute transport equations consider advective-dispersive transport in the liquid phase. The program can simulate a broad range of low-temperature biogeochemical reactions in water, soil and ground water systems including interactions with minerals, gases, exchangers, and sorption surfaces, based on thermodynamic equilibrium, kinetics, or mixed equilibrium-kinetic reactions. The program may be used to analyze water and solute movement in unsaturated, partially saturated, or fully saturated porous media. The flow region may be composed of nonuniform soils or sediments. Flow and transport can occur in the vertical, horizontal, or a generally inclined direction. The water flow part of the model can deal with prescribed head and flux boundaries, boundaries controlled by atmospheric conditions, as well as free drainage boundary conditions. The governing flow and transport equations were solved numerically using Galerkin-type linear finite element schemes. To test the accuracy of the coupling procedures implemented in HP1, simulation results were compared with (i) HYDRUS-1D for transport problems of multiple components subject to sequential first-order decay, (ii) PHREEQC for steady-state flow conditions, and

HPC Institutional Computing Project: W15_lesreactiveflow KIVA-hpFE Development: A Robust and Accurate Engine Modeling Software

Energy Technology Data Exchange (ETDEWEB)

Carrington, David Bradley [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Waters, Jiajia [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-01-05

KIVA-hpFE is a high performance computer software for solving the physics of multi-species and multiphase turbulent reactive flow in complex geometries having immersed moving parts. The code is written in Fortran 90/95 and can be used on any computer platform with any popular complier. The code is in two versions, a serial version and a parallel version utilizing MPICH2 type Message Passing Interface (MPI or Intel MPI) for solving distributed domains. The parallel version is at least 30x faster than the serial version and much faster than our previous generation of parallel engine modeling software, by many factors. The 5th generation algorithm construction is a Galerkin type Finite Element Method (FEM) solving conservative momentum, species, and energy transport equations along with two-equation turbulent model k-ω Reynolds Averaged Navier-Stokes (RANS) model and a Vreman type dynamic Large Eddy Simulation (LES) method. The LES method is capable modeling transitional flow from laminar to fully turbulent; therefore, this LES method does not require special hybrid or blending to walls. The FEM projection method also uses a Petrov-Galerkin (P-G) stabilization along with pressure stabilization. We employ hierarchical basis sets, constructed on the fly with enrichment in areas associated with relatively larger error as determined by error estimation methods. In addition, when not using the hp-adaptive module, the code employs Lagrangian basis or shape functions. The shape functions are constructed for hexahedral, prismatic and tetrahedral elements. The software is designed to solve many types of reactive flow problems, from burners to internal combustion engines and turbines. In addition, the formulation allows for direct integration of solid bodies (conjugate heat transfer), as in heat transfer through housings, parts, cylinders. It can also easily be extended to stress modeling of solids, used in fluid structure interactions problems, solidification, porous media

Analysis and development of spatial hp-refinement methods for solving the neutron transport equation; Analyse et developpement de methodes de raffinement hp en espace pour l'equation de transport des neutrons

Energy Technology Data Exchange (ETDEWEB)

Fournier, D.

2011-10-10

The different neutronic parameters have to be calculated with a higher accuracy in order to design the 4. generation reactor cores. As memory storage and computation time are limited, adaptive methods are a solution to solve the neutron transport equation. The neutronic flux, solution of this equation, depends on the energy, angle and space. The different variables are successively discretized. The energy with a multigroup approach, considering the different quantities to be constant on each group, the angle by a collocation method called SN approximation. Once the energy and angle variable are discretized, a system of spatially-dependent hyperbolic equations has to be solved. Discontinuous finite elements are used to make possible the development of hp-refinement methods. Thus, the accuracy of the solution can be improved by spatial refinement (h-refinement), consisting into subdividing a cell into sub-cells, or by order refinement (p-refinement), by increasing the order of the polynomial basis. In this thesis, the properties of this methods are analyzed showing the importance of the regularity of the solution to choose the type of refinement. Thus, two error estimators are used to lead the refinement process. Whereas the first one requires high regularity hypothesis (analytical solution), the second one supposes only the minimal hypothesis required for the solution to exist. The comparison of both estimators is done on benchmarks where the analytic solution is known by the method of manufactured solutions. Thus, the behaviour of the solution as a regard of the regularity can be studied. It leads to a hp-refinement method using the two estimators. Then, a comparison is done with other existing methods on simplified but also realistic benchmarks coming from nuclear cores. These adaptive methods considerably reduces the computational cost and memory footprint. To further improve these two points, an approach with energy-dependent meshes is proposed. Actually, as the

Thermodynamic studies at the low-dimensional spin systems HP-(VO)2P2O7, SrCu2(BO3)2, and azurite

International Nuclear Information System (INIS)

Bruehl, Andreas

2007-01-01

The present thesis deals with the low-temperature properties of three low-dimensional spin systems. The main experimental topic lies on measurements on the thermal expansion and on the specific heat, which were performed with a high-resolution capacitative dilatometer respectively an AC calorimeter facility. Because the so-called magnetic Grueneisen parameters, which describe the strength of the coupling of the magnetic partial system to the lattice, are throughout very large, especially the thermal-expansion measurements yield valuable information on the understanding of the treated systems. The central result of this thesis represent measurements on the high-pressure phase of (VO) 2 P 2 O 7 , briefly PP-VOPO. This system consists of alternating spin chains, whereby both exchange constants have similar values, i. e. only a weak alternation is present. In the thermal expansion an anomaly especially pronounced in chain direction at about 13 K. From the Grueneisen parameters determined by application of this model it can be concluded that the pronounced low-temperature anomaly in HP-VOPO is partly caused by the strong deformation dependence of the smaller of the two exchange constants, but partly also on the neighbourhood to a quantum critical point. The two-dimensional dimer system SrCu 2 (BO 3 ) 2 has gotten fame by the localization of the triplet excitations and the magnetization plateaus at certain fractions of the saturation magnetization conditioned by this. In the thermal expansion a distinct anomaly at the same temperature (T=8 K) is observed, as it also occurs in the specific heat. Finally measurements at the natural mineral azurite are presented, in which the spin are arranged in so-called diamond chains. In the magnetic susceptibility, the specific heat, and the thermal expansion a remarkable double structure occurs. Also the Λ-shaped antiferromagnetic order transition was studied and the phase diagram, consisting of paramagnetic, antiferromagnetic, and

Hyperpolarized Amino Acid Derivatives as Multivalent Magnetic Resonance pH Sensor Molecules

Directory of Open Access Journals (Sweden)

Christian Hundshammer

2018-02-01

Full Text Available pH is a tightly regulated physiological parameter that is often altered in diseased states like cancer. The development of biosensors that can be used to non-invasively image pH with hyperpolarized (HP magnetic resonance spectroscopic imaging has therefore recently gained tremendous interest. However, most of the known HP-sensors have only individually and not comprehensively been analyzed for their biocompatibility, their pH sensitivity under physiological conditions, and the effects of chemical derivatization on their logarithmic acid dissociation constant (pKa. Proteinogenic amino acids are biocompatible, can be hyperpolarized and have at least two pH sensitive moieties. However, they do not exhibit a pH sensitivity in the physiologically relevant pH range. Here, we developed a systematic approach to tailor the pKa of molecules using modifications of carbon chain length and derivatization rendering these molecules interesting for pH biosensing. Notably, we identified several derivatives such as [1-13C]serine amide and [1-13C]-2,3-diaminopropionic acid as novel pH sensors. They bear several spin-1/2 nuclei (13C, 15N, 31P with high sensitivity up to 4.8 ppm/pH and we show that 13C spins can be hyperpolarized with dissolution dynamic polarization (DNP. Our findings elucidate the molecular mechanisms of chemical shift pH sensors that might help to design tailored probes for specific pH in vivo imaging applications.

Hyperpolarized Amino Acid Derivatives as Multivalent Magnetic Resonance pH Sensor Molecules.

Science.gov (United States)

Hundshammer, Christian; Düwel, Stephan; Ruseckas, David; Topping, Geoffrey; Dzien, Piotr; Müller, Christoph; Feuerecker, Benedikt; Hövener, Jan B; Haase, Axel; Schwaiger, Markus; Glaser, Steffen J; Schilling, Franz

2018-02-15

pH is a tightly regulated physiological parameter that is often altered in diseased states like cancer. The development of biosensors that can be used to non-invasively image pH with hyperpolarized (HP) magnetic resonance spectroscopic imaging has therefore recently gained tremendous interest. However, most of the known HP-sensors have only individually and not comprehensively been analyzed for their biocompatibility, their pH sensitivity under physiological conditions, and the effects of chemical derivatization on their logarithmic acid dissociation constant (p K a ). Proteinogenic amino acids are biocompatible, can be hyperpolarized and have at least two pH sensitive moieties. However, they do not exhibit a pH sensitivity in the physiologically relevant pH range. Here, we developed a systematic approach to tailor the p K a of molecules using modifications of carbon chain length and derivatization rendering these molecules interesting for pH biosensing. Notably, we identified several derivatives such as [1- 13 C]serine amide and [1- 13 C]-2,3-diaminopropionic acid as novel pH sensors. They bear several spin-1/2 nuclei ( 13 C, 15 N, 31 P) with high sensitivity up to 4.8 ppm/pH and we show that 13 C spins can be hyperpolarized with dissolution dynamic polarization (DNP). Our findings elucidate the molecular mechanisms of chemical shift pH sensors that might help to design tailored probes for specific pH in vivo imaging applications.

Monocrotophos, an organophosphorus insecticide, disrupts the expression of HpNetrin and its receptor neogenin during early development in the sea urchin (Hemicentrotus pulcherrimus).

Science.gov (United States)

Zhang, Xiaona; Xu, Lei; Tian, Hua; Wang, Cuicui; Wang, Wei; Ru, Shaoguo

2017-09-01

Netrins, chemotropic guidance cues, can guide the extension of serotonergic axons by binding to netrin receptors during neural development. However, little is known about whether disruption of netrin signaling is involved in the mechanisms by which organophosphorus pesticides affect serotonergic nervous system (SNS) development. In this study, we evaluated the effects of the pesticide monocrotophos (MCP) on the expression patterns of HpNetrin and its receptor neogenin as well as on the intracellular calcium ion (Ca 2+ ) levels in Hemicentrotus pulcherrimus (sea urchin) by exposing fertilized embryos to 0, 0.01, 0.10, and 1.00mg/L MCP. The results showed that MCP disrupted HpNetrin and neogenin expression at different developmental stages in H. pulcherrimus and that Ca 2+ appeared to be involved in the MCP-induced developmental neurotoxicity. Specifically, the lower concentrations of MCP elevated HpNetrin and neogenin transcription, resulting in higher intracellular Ca 2+ levels during the early developmental stages in the sea urchin; this may affect netrin-directed cell migration/axon extension and subsequently disrupt serotonergic axon branching and synapse formation. In contrast, 1.00mg/L MCP exhibited an inhibitory effect on HpNetrin and neogenin transcription. This finding implies that the regulatory roles of these factors may be diminished during early development, thereby causing developmental defects in the sea urchin. Collectively, our results provide a basis for exploring the involvement of netrin and neogenin in the organophosphate-induced disruption of the SNS during development. Copyright © 2017 Elsevier B.V. All rights reserved.

Recruitment of the cohesin loading factor NIPBL to DNA double-strand breaks depends on MDC1, RNF168 and HP1γ in human cells

International Nuclear Information System (INIS)

Oka, Yasuyoshi; Suzuki, Keiji; Yamauchi, Motohiro; Mitsutake, Norisato; Yamashita, Shunichi

2011-01-01

Highlights: → NIPBL is recruited to DSBs. → Localization of NIPBL to DSBs is regulated by MDC1 and RNF168. → HP1γ is required for NIPBL accumulation at DSBs. -- Abstract: The cohesin loading factor NIPBL is required for cohesin to associate with chromosomes and plays a role in DNA double-strand break (DSB) repair. Although the NIPBL homolog Scc2 is recruited to an enzymatically generated DSB and promotes cohesin-dependent DSB repair in yeast, the mechanism of the recruitment remains poorly understood. Here we show that the human NIPBL is recruited to the sites of DNA damage generated by micro-irradiation as well as to the sites of DSBs induced by homing endonuclease, I-PpoI. The recruitment of NIPBL was impaired by RNAi-mediated knockdown of MDC1 or RNF168, both of which also accumulate at DSBs. We also show that the recruitment of NIPBL to the sites of DNA damage is mediated by its C-terminal region containing HEAT repeats and Heterochromatin protein 1 (HP1) interacting motif. Furthermore, NIPBL accumulation at damaged sites was also compromised by HP1γ depletion. Taken together, our study reveals that human NIPBL is a novel protein recruited to DSB sites, and the recruitment is controlled by MDC1, RNF168 and HP1γ.

Assessment of four calculation methods proposed by the EC for waste hazardous property HP 14 'Ecotoxic'.

Science.gov (United States)

Hennebert, Pierre; Humez, Nicolas; Conche, Isabelle; Bishop, Ian; Rebischung, Flore

2016-02-01

Legislation published in December 2014 revised both the List of Waste (LoW) and amended Appendix III of the revised Waste Framework Directive 2008/98/EC; the latter redefined hazardous properties HP 1 to HP 13 and HP 15 but left the assessment of HP 14 unchanged to allow time for the Directorate General of the Environment of the European Commission to complete a study that is examining the impacts of four different calculation methods for the assessment of HP 14. This paper is a contribution to the assessment of the four calculation methods. It also includes the results of a fifth calculation method; referred to as "Method 2 with extended M-factors". Two sets of data were utilised in the assessment; the first (Data Set #1) comprised analytical data for 32 different waste streams (16 hazardous (H), 9 non-hazardous (NH) and 7 mirror entries, as classified by the LoW) while the second data set (Data Set #2), supplied by the eco industries, comprised analytical data for 88 waste streams, all classified as hazardous (H) by the LoW. Two approaches were used to assess the five calculation methods. The first approach assessed the relative ranking of the five calculation methods by the frequency of their classification of waste streams as H. The relative ranking of the five methods (from most severe to less severe) is: Method 3>Method 1>Method 2 with extended M-factors>Method 2>Method 4. This reflects the arithmetic ranking of the concentration limits of each method when assuming M=10, and is independent of the waste streams, or the H/NH/Mirror status of the waste streams. A second approach is the absolute matching or concordance with the LoW. The LoW is taken as a reference method and the H wastes are all supposed to be HP 14. This point is discussed in the paper. The concordance for one calculation method is established by the number of wastes with identical classification by the considered calculation method and the LoW (i.e. H to H, NH to NH). The discordance is

Drosophila atm/telomere fusion is required for telomeric localization of HP1 and telomere position effect.

Science.gov (United States)

Oikemus, Sarah R; McGinnis, Nadine; Queiroz-Machado, Joana; Tukachinsky, Hanna; Takada, Saeko; Sunkel, Claudio E; Brodsky, Michael H

2004-08-01

Terminal deletions of Drosophila chromosomes can be stably protected from end-to-end fusion despite the absence of all telomere-associated sequences. The sequence-independent protection of these telomeres suggests that recognition of chromosome ends might contribute to the epigenetic protection of telomeres. In mammals, Ataxia Telangiectasia Mutated (ATM) is activated by DNA damage and acts through an unknown, telomerase-independent mechanism to regulate telomere length and protection. We demonstrate that the Drosophila homolog of ATM is encoded by the telomere fusion (tefu) gene. In the absence of ATM, telomere fusions occur even though telomere-specific Het-A sequences are still present. High levels of spontaneous apoptosis are observed in ATM-deficient tissues, indicating that telomere dysfunction induces apoptosis in Drosophila. Suppression of this apoptosis by p53 mutations suggests that loss of ATM activates apoptosis through a DNA damage-response mechanism. Loss of ATM reduces the levels of heterochromatin protein 1 (HP1) at telomeres and suppresses telomere position effect. We propose that recognition of chromosome ends by ATM prevents telomere fusion and apoptosis by recruiting chromatin-modifying complexes to telomeres.

Thermal stress state of cryogenic HP vessels under freezing and pressurization

International Nuclear Information System (INIS)

Tsybenko, A.S.; Kuranov, B.A.; Chepurnoj, A.D.; Shaposhnikov, V.A.; Krishchuk, N.G.

1986-01-01

A mathematical model is developed for thermomechanical processes in cryogenic HP vessels under freezing either by liquid and (or) gaseous cryogen and under pressurization. Equations of nonlinear nonstationary thermal conductivity and nonisothermal thermoelastoplasticity are used for the case of the theory off low with isotropic hardening. Semiempiricaldependences of nonstationary heat exchange for gaseous medium, experimental curves of cryogenic liquid boiling, mass exchange relationships are allowed for when formulating boundary conditions. The mathematical modelis realized on the basi of the finite element method in the form of highly automated program complex TERSOD (heat resistanceof vessels), oriented for computer of the Unified System. Heat and stress-strained states for three constructions of vessels are thoroughly studied under different conditions of gaseous, liquid and combined freezing with subsequent pressurization

Outer membrane protein P4 is not required for virulence in the human challenge model of Haemophilus ducreyi infection.

Science.gov (United States)

Janowicz, Diane M; Zwickl, Beth W; Fortney, Kate R; Katz, Barry P; Bauer, Margaret E

2014-06-24

Bacterial lipoproteins often play important roles in pathogenesis and can stimulate protective immune responses. Such lipoproteins are viable vaccine candidates. Haemophilus ducreyi, which causes the sexually transmitted disease chancroid, expresses a number of lipoproteins during human infection. One such lipoprotein, OmpP4, is homologous to the outer membrane lipoprotein e (P4) of H. influenzae. In H. influenzae, e (P4) stimulates production of bactericidal and protective antibodies and contributes to pathogenesis by facilitating acquisition of the essential nutrients heme and nicotinamide adenine dinucleotide (NAD). Here, we tested the hypothesis that, like its homolog, H. ducreyi OmpP4 contributes to virulence and stimulates production of bactericidal antibodies. We determined that OmpP4 is broadly conserved among clinical isolates of H. ducreyi. We next constructed and characterized an isogenic ompP4 mutant, designated 35000HPompP4, in H. ducreyi strain 35000HP. To test whether OmpP4 was necessary for virulence in humans, eight healthy adults were experimentally infected. Each subject was inoculated with a fixed dose of 35000HP on one arm and three doses of 35000HPompP4 on the other arm. The overall parent and mutant pustule formation rates were 52.4% and 47.6%, respectively (P = 0.74). These results indicate that expression of OmpP4 in not necessary for H. ducreyi to initiate disease or progress to pustule formation in humans. Hyperimmune mouse serum raised against purified, recombinant OmpP4 did not promote bactericidal killing of 35000HP or phagocytosis by J774A.1 mouse macrophages in serum bactericidal and phagocytosis assays, respectively. Our data suggest that, unlike e (P4), H. ducreyi OmpP4 is not a suitable vaccine candidate. OmpP4 may be dispensable for virulence because of redundant mechanisms in H. ducreyi for heme acquisition and NAD utilization.

Adenine Nucleotide Analogues Locked in a Northern Methanocarba Conformation: Enhanced Stability and Potency as P2Y1 Receptor Agonists

Science.gov (United States)

Ravi, R. Gnana; Kim, Hak Sung; Servos, Jörg; Zimmermann, Herbert; Lee, Kyeong; Maddileti, Savitri; Boyer, José L.; Harden, T. Kendall; Jacobson, Kenneth A.

2016-01-01

Preference for the Northern (N) ring conformation of the ribose moiety of nucleotide 5′-triphosphate agonists at P2Y1, P2Y2, P2Y4, and P2Y11 receptors, but not P2Y6 receptors, was established using a ring-constrained methanocarba (a 3.1.0-bicyclohexane) ring as a ribose substitute (Kim et al. J. Med. Chem. 2002, 45, 208–218.). We have now combined the ring-constrained (N)-methanocarba modification of adenine nucleotides with other functionalities known to enhance potency at P2 receptors. The potency of the newly synthesized analogues was determined in the stimulation of phospholipase C through activation of turkey erythrocyte P2Y1 or human P2Y1 and P2Y2 receptors stably expressed in astrocytoma cells. An (N)-methanocarba-2-methylthio-ADP analogue displayed an EC50 at the hP2Y1 receptor of 0.40 nM and was 55-fold more potent than the corresponding triphosphate and 16-fold more potent than the riboside 5′-diphosphate. 2-Cl–(N)-methanocarba-ATP and its N6-Me analogue were also highly selective, full agonists at P2Y1 receptors. The (N)-methanocarba-2-methylthio and 2-chloromonophosphate analogues were full agonists exhibiting micromolar potency at P2Y1 receptors, while the corresponding ribosides were inactive. Although β,γ-methylene-ATP was inactive at P2Y receptors, β,γ-methylene-(N)-methanocarba-ATP was a potent hP2Y1 receptor agonist with an EC50 of 160 nM and was selective versus hP2Y2 and hP2Y4 receptors. The rates of hydrolysis of Northern (N) and Southern (S) methanocarba analogues of AMP by rat 5′-ectonucleotidase were negligible. The rates of hydrolysis of the corresponding triphosphates by recombinant rat NTPDase1 and 2 were studied. Both isomers were hydrolyzed by NTPDase 1 at about half the rate of ATP hydrolysis. The (N) isomer was hardly hydrolyzed by NTPDase 2, while the (S) isomer was hydrolyzed at one-third of the rate of ATP hydrolysis. This suggests that new, more stable and selective nucleotide agonists may be designed on the basis of

Use of a Parabolic Microphone to Detect Hidden Subjects in Search and Rescue.

Science.gov (United States)

Bowditch, Nathaniel L; Searing, Stanley K; Thomas, Jeffrey A; Thompson, Peggy K; Tubis, Jacqueline N; Bowditch, Sylvia P

2018-03-01

This study compares a parabolic microphone to unaided hearing in detecting and comprehending hidden callers at ranges of 322 to 2510 m. Eight subjects were placed 322 to 2510 m away from a central listening point. The subjects were concealed, and their calling volume was calibrated. In random order, subjects were asked to call the name of a state for 5 minutes. Listeners with parabolic microphones and others with unaided hearing recorded the direction of the call (detection) and name of the state (comprehension). The parabolic microphone was superior to unaided hearing in both detecting subjects and comprehending their calls, with an effect size (Cohen's d) of 1.58 for detection and 1.55 for comprehension. For each of the 8 hidden subjects, there were 24 detection attempts with the parabolic microphone and 54 to 60 attempts by unaided listeners. At the longer distances (1529-2510 m), the parabolic microphone was better at detecting callers (83% vs 51%; P<0.00001 by χ 2 ) and comprehension (57% vs 12%; P<0.00001). At the shorter distances (322-1190 m), the parabolic microphone offered advantages in detection (100% vs 83%; P=0.000023) and comprehension (86% vs 51%; P<0.00001), although not as pronounced as at the longer distances. Use of a 66-cm (26-inch) parabolic microphone significantly improved detection and comprehension of hidden calling subjects at distances between 322 and 2510 m when compared with unaided hearing. This study supports the use of a parabolic microphone in search and rescue to locate responsive subjects in favorable weather and terrain. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Defining the best parallelization strategy for a diphasic compressible fluid mechanics code

International Nuclear Information System (INIS)

Berthou, Jean-Yves; Fayolle, Eric; Faucher, Eric; Scliffet, Laurent

2000-01-01

Nuclear plants use steam generator safety valves in order to regulate possible large pressure variations of fluids. In case of an incident these valves may be fed with pressurized liquid water (for instance a pressure of 9 MPa at a temperature of 300degC). When a pressurized liquid is submitted to a strong pressure drop, it will start evaporating. This phenomena is called flashing. Z. Bilicki and co-authors proposed the homogeneous relaxation model (HRM) to compute critical flashing water flows. Its computation in the case of non stationary one-dimensional flashing flows has been carried out with the development of a dedicated time dependent Finite Volume scheme based on a simplified version of the Godunov approach. Electricite De France Research and Development division have developed a monodimensional implementation of the HRM model: ECOSS, a 11000 lines FORTRAN 90. Applied to a shock tube test case with a 20000 elements monodimensional mesh, the simulation of the physical phenomenon during 2.5 seconds requires at least 100 days of computation on a SUN Sparc-Ultra60. This execution time justifies the ECOSS parallelization. Furthermore, we plan a modeling on 2D meshes for the next few years. Knowing that multiplying the mesh dimension by a factor 10 multiplies the execution time by a factor 100, ECOSS would take years of computation with small 2D meshes (1000 x 1000) on a conventional workstation. This paper describes the parallelization analysis we have conducted and we presents the experimental results we have obtained applying different programming model (MPI, OpenMP, HPF) on various platforms (a Compaq Proliant 6000 4 processors, a Cray T3E-750 300 processors, a HP class V 16 processors, a SGI Origin2000 32 processors, a cluster of PCs and a COMPAQ SC 232 processors). These experimental results will be discussed according to the following criteria: efficiency, salability, maintainability, developing costs and portability. As a conclusion, we will present the

Defining the best parallelization strategy for a diphasic compressible fluid mechanics code

Energy Technology Data Exchange (ETDEWEB)

Berthou, Jean-Yves; Fayolle, Eric [Electricite de France, Research and Development division, Modeling and Information Technologies Department, CLAMART CEDEX (France); Faucher, Eric; Scliffet, Laurent [Electricite de France, Research and Development Division, Mechanics and Component Technology Branch Department, Moret sur Loing (France)

2000-09-01

Nuclear plants use steam generator safety valves in order to regulate possible large pressure variations of fluids. In case of an incident these valves may be fed with pressurized liquid water (for instance a pressure of 9 MPa at a temperature of 300degC). When a pressurized liquid is submitted to a strong pressure drop, it will start evaporating. This phenomena is called flashing. Z. Bilicki and co-authors proposed the homogeneous relaxation model (HRM) to compute critical flashing water flows. Its computation in the case of non stationary one-dimensional flashing flows has been carried out with the development of a dedicated time dependent Finite Volume scheme based on a simplified version of the Godunov approach. Electricite De France Research and Development division have developed a monodimensional implementation of the HRM model: ECOSS, a 11000 lines FORTRAN 90. Applied to a shock tube test case with a 20000 elements monodimensional mesh, the simulation of the physical phenomenon during 2.5 seconds requires at least 100 days of computation on a SUN Sparc-Ultra60. This execution time justifies the ECOSS parallelization. Furthermore, we plan a modeling on 2D meshes for the next few years. Knowing that multiplying the mesh dimension by a factor 10 multiplies the execution time by a factor 100, ECOSS would take years of computation with small 2D meshes (1000 x 1000) on a conventional workstation. This paper describes the parallelization analysis we have conducted and we presents the experimental results we have obtained applying different programming model (MPI, OpenMP, HPF) on various platforms (a Compaq Proliant 6000 4 processors, a Cray T3E-750 300 processors, a HP class V 16 processors, a SGI Origin2000 32 processors, a cluster of PCs and a COMPAQ SC 232 processors). These experimental results will be discussed according to the following criteria: efficiency, salability, maintainability, developing costs and portability. As a conclusion, we will present the

LATE TRIASSIC OBLIQUE EXTRUSION OF UHP/HP COMPLEXES IN THE ATBASHI ACCRETIONARY COMPLEX OF SOUTH TIANSHAN, KYRGYZSTAN

Directory of Open Access Journals (Sweden)

Wenjiao Xiao

2017-01-01

Full Text Available The exhumation and tectonic emplacement of eclogites and blueschists take place in forearc accretionary complexes by either forearc- or backarc-directed extrusion, but few examples have been well analysed in detail. Here we present an example of oblique wedge extrusion of UHP/HP rocks in the Atbashi accretionary complex of the Kyrgyz South Tianshan.

Synergistic effect of elevated temperature, pCO2 and nutrients on marine biofilm

Digital Repository Service at National Institute of Oceanography (India)

Baragi, L.V.; Anil, A.C.

the respiration and in turn metabolic and energy cost of bacteria (Del Giorgio et al., 1999; Siu et al., 2014). This highlights the significant influence of nutrients on the response of bacteria to ocean acidification. Elevated temperature, irrespective of p...., Thyrhaug, R., Grossart, H.-P., 2008. Coupling of heterotrophic bacteria to phytoplankton bloom development at different pCO2 levels: a mesocosm study. Biogeosciences 5, 1007-1022. Apple, J.K., Del Giorgio, P., Kemp, W.M., 2006. Temperature regulation...

Progression of Emphysema Evaluated by MRI Using Hyperpolarized 3He (HP 3He Measurements in Patients with Alpha-1-Antitrypsin (A1AT) Deficiency Compared with CT and Lung Function Tests

International Nuclear Information System (INIS)

Stavngaard, T.; Vejby Soegaard, L.; Batz, M.; Schreiber, L.M.; Dirksen, A.

2009-01-01

Background: The progression of emphysema is traditionally measured by pulmonary function test, with forced expiratory volume in 1 s (FEV1) being the most accepted and used measurement. However, FEV1 is insensitive in detecting mild/slow progression of emphysema because of low reproducibility as compared to yearly decline. Purpose: To investigate the progression of emphysema over a period of 2 years using diffusion-weighted hyperpolarized (HP) 3 He magnetic resonance imaging (MRI) in patients with alpha-1-antitrypsin (A1AT) deficiency. Material and Methods: Nine patients with severe A1AT deficiency were studied over a period of 2 years (baseline, year 1, and year 2) with HP 3 He MRI using apparent diffusion coefficient (ADC), lung function tests (FEV1 and carbon monoxide lung diffusion capacity [DL,CO]), and computed tomography (CT) using densitometric parameters (15th percentile density [CT-PD15] and relative area of emphysema below -910 HU [CT-RA-910]). Results: Seven patients were scanned three times, one patient two times, and one patient only at baseline. The mean increase in ADC values from first to last HP 3 He MR scanning was 3.8% (0.014 cm 2 /s [SD 0.024 cm 2 /s]; not significant). The time trends for FEV1, DL,CO, CT-PD15, and CT-RA-910 were all statistically significant. We found a high correlation between ADC and DL,CO (P 3 He MRI for monitoring the progression of emphysema. However, in the future, larger studies are needed to confirm these preliminary results

Negative-tone imaging with EUV exposure toward 13nm hp

Science.gov (United States)

Tsubaki, Hideaki; Nihashi, Wataru; Tsuchihashi, Toru; Yamamoto, Kei; Goto, Takahiro

2016-03-01

Negative-tone imaging (NTI) with EUV exposure has major advantages with respect to line-width roughness (LWR) and resolution due in part to polymer swelling and favorable dissolution mechanics. In NTI process, both resist and organic solvents play important roles in determining lithography performances. The present study describes novel chemically amplified resist materials based on NTI technology with EUV using a specific organic solvents. Lithographic performances of NTI process were described in this paper under exposures using ASML NXE:3300 EUV scanner at imec. It is emphasized that 14 nm hp was nicely resolved under exposure dose of 37 mJ/cm2 without any bridge and collapse, which are attributed to the low swelling character of NTI process. Although 13 nm hp resolution was potentially obtained, a pattern collapse still restricts its resolution in case coating resist film thickness is 40 nm. Dark mask limitation due mainly to mask defectivity issue makes NTI with EUV favorable approach for printing block mask to produce logic circuit. A good resolution of CD-X 21 nm/CD-Y 32 nm was obtained for block mask pattern using NTI with usable process window and dose of 49 mJ/cm2. Minimum resolution now reaches CD-X 17 nm / CD-Y 23 nm for the block. A 21 nm block mask resolution was not affected by exposure dose and explored toward low dose down to 18 mJ/cm2 by reducing quencher loading. In addition, there was a negligible amount of increase in LCDU for isolated dot pattern when decreasing exposure dose from 66 mJ/cm2 to 24 mJ/cm2. On the other hand, there appeared tradeoff relationship between LCDU and dose for dense dot pattern, indicating photon-shot noise restriction, but strong dependency on patterning features. Design to improve acid generation efficiency was described based on acid generation mechanism in traditional chemically amplified materials which contains photo-acid generator (PAG) and polymer. Conventional EUV absorber comprises of organic compounds is

hp-version discontinuous Galerkin methods on polygonal and polyhedral meshes

CERN Document Server

Cangiani, Andrea; Georgoulis, Emmanuil H; Houston, Paul

2017-01-01

Over the last few decades discontinuous Galerkin finite element methods (DGFEMs) have been witnessed tremendous interest as a computational framework for the numerical solution of partial differential equations. Their success is due to their extreme versatility in the design of the underlying meshes and local basis functions, while retaining key features of both (classical) finite element and finite volume methods. Somewhat surprisingly, DGFEMs on general tessellations consisting of polygonal (in 2D) or polyhedral (in 3D) element shapes have received little attention within the literature, despite the potential computational advantages. This volume introduces the basic principles of hp-version (i.e., locally varying mesh-size and polynomial order) DGFEMs over meshes consisting of polygonal or polyhedral element shapes, presents their error analysis, and includes an extensive collection of numerical experiments. The extreme flexibility provided by the locally variable elemen t-shapes, element-sizes, and elemen...

Microstructure of Magnesium alloy AZ91-HP cast in permanent and non-permanent moulds

DEFF Research Database (Denmark)

Tiedje, Niels; Sørensen, Rasmus Kirkegaard; Albachari, Sami

2003-01-01

Casting of different geometries were made with AZ91-HP in different moulding media, so that a range of cooling conditions were obtained. Cooling curves were measured and compared to the microstructures found in the castings. It was shown that segregation in the alloy is highly dependent on cooling...... rate. This leads to precipitation of varying amounts of intermetallic phases. The distribution of phases in the material is controlled both by interdendritic and intergranular segregation, leading to an inhomogeneous microstructure. It is shown that local solidification time, segregation and diffusion...

An hp symplectic pseudospectral method for nonlinear optimal control

Science.gov (United States)

Peng, Haijun; Wang, Xinwei; Li, Mingwu; Chen, Biaosong

2017-01-01

An adaptive symplectic pseudospectral method based on the dual variational principle is proposed and is successfully applied to solving nonlinear optimal control problems in this paper. The proposed method satisfies the first order necessary conditions of continuous optimal control problems, also the symplectic property of the original continuous Hamiltonian system is preserved. The original optimal control problem is transferred into a set of nonlinear equations which can be solved easily by Newton-Raphson iterations, and the Jacobian matrix is found to be sparse and symmetric. The proposed method, on one hand, exhibits exponent convergence rates when the number of collocation points are increasing with the fixed number of sub-intervals; on the other hand, exhibits linear convergence rates when the number of sub-intervals is increasing with the fixed number of collocation points. Furthermore, combining with the hp method based on the residual error of dynamic constraints, the proposed method can achieve given precisions in a few iterations. Five examples highlight the high precision and high computational efficiency of the proposed method.

Sphingomonas frigidaeris sp. nov., isolated from an air conditioning system.

Science.gov (United States)

Lee, Yunho; Jeon, Che Ok

2017-10-01

A strictly aerobic Gram-stain-negative bacterium, designated strain KER25-10 T , was isolated from a laboratory air conditioning system in South Korea. Cells were yellow-pigmented, non-motile rods showing catalase- and oxidase-positive reactions. The strain grew at pH 4.0-9.0 (optimum, pH 6.0-7.0) and 10-40 °C (optimum, 30 °C) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %). The G+C content of the genomic DNA was 65.1 mol%. Strain KER25-10 T contained ubiquinone-10 (Q-10) as the predominant isoprenoid quinone and C16 : 0, C17 : 1ω6c, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) as the major fatty acids. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Only spermidine was detected as the polyamine. Phylogenetic analysis based on 16S rRNA sequences indicated that strain KER25-10 T formed a distinct phylogenetic lineage within the genus Sphingomonas of the family Sphingomonadaceae and the strain was most closely related to Sphingomonas kyeonggiense THG-DT81 T with a 96.8 % 16S rRNA gene sequence similarity. On the basis of phenotypic, chemotaxonomic and molecular features, strain KER25-10 T clearly represents a novel species of the genus Sphingomonas, for which the name Sphingomonas frigidaeris sp. nov. is proposed. The type strain is KER25-10 T (=KACC 19285 T =JCM 32053 T ).

Mechanical behaviour of Astm A 297 grade Hp joints welded using different processes

International Nuclear Information System (INIS)

Emygdio, Paulo Roberto Oliveira; Zeemann, Annelise; Almeida, Luiz Henrique de

1996-01-01

The influence of different arc welding processes on mechanical behaviour was studied for cast heat resistant stainless steel welded joints, in the as welded conditions. ASTM A 297 grade HP with niobium and niobium/titanium additions were welded following three different welding procedures, using shielded metal arc welding gas tungsten arc welding and plasma arc welding, in six welded joints. The welded joint mechanical behaviour was evaluated by ambient temperature and 870 deg C tensile tests; and creep tests at 900 deg C and 50 MPa. Mechanical test results showed that the welding procedure qualification following welding codes is not suitable for high temperature service applications. (author)

Proposal of concentration limits for determining the hazard property HP 14 for waste using ecotoxicological tests.

Science.gov (United States)

Hennebert, Pierre

2018-04-01

Different ecotoxicological test batteries and concentration limits have been proposed to assess the hazard property (HP) HP 14 'Ecotoxic' for waste in the European Union and its member states. In test batteries, if the concentration of waste in the culture/dilution medium producing 50% of inhibitory biological effect in one or more test(s) is below the concentration limit of the test, the waste is classified as hazardous. A summarized review of the test batteries proposed since 1998 is presented. The last proposed test battery uses seven aquatic and terrestrial species with standardized methods, but with options and uniform concentration limits of 10% of waste eluate or solid waste in the culture/dilution medium. No attempt was made to match this hazard assessment with the classification made in the European List of Waste (LoW). The aim of this paper is to propose for the same test battery (reduced to 6 tests without options) concentration limits that match with the European List of Waste. This list was taken as reference (despite the fact that waste can be hazardous for other properties than the most frequent HP 14, and its partly political nature for some opinions). The concentration limits (CLs) for tests are the concentrations producing the highest ecotoxicological effects for each test observed in a non-hazardous waste set. Data from Germany, France and Belgium (from in total 5 different sources from 2009 to 2016) with the above-mentioned test battery (without options) were gathered for 81 samples, being the largest set ever published. In total, ten non-hazardous (NH) waste samples (as defined by the LoW and for most of them checked by chemical composition) were used to establish CLs. These CLs were then applied to 13 hazardous (H) waste by the LoW, and all were classified as hazardous. The matching of the resulting classification with the LoW is convincing. For the 58 'mirror entries' in the LoW (hazardous or not depending of the presence of hazardous

Investigation of HP Turbine Blade Failure in a Military Turbofan Engine

Science.gov (United States)

Mishra, R. K.; Thomas, Johny; Srinivasan, K.; Nandi, Vaishakhi; Bhatt, R. Raghavendra

2017-04-01

Failure of a high pressure (HP) turbine blade in a military turbofan engine is investigated to determine the root cause of failure. Forensic and metallurgical investigations are carried out on the affected blades. The loss of coating and the presence of heavily oxidized intergranular fracture features including substrate material aging and airfoil curling in the trailing edge of a representative blade indicate that the coating is not providing adequate oxidation protection and the blade material substrate is not suitable for the application at hand. Coating spallation followed by substrate oxidation and aging leading to intergranular cracking and localized trailing edge curling is the root cause of the blade failure. The remaining portion of the blade fracture surface showed ductile overload features in the final failure. The damage observed in downstream components is due to secondary effects.

Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha

NARCIS (Netherlands)

Leão-Helder, Adriana N; Krikken, Arjen M; Gellissen, Gerd; van der Klei, Ida J; Veenhuis, Marten; Kiel, Jan A K W

2004-01-01

ATG genes are required for autophagy-related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp-Atg21p, fused to eGFP, had a dual

3He(d,p)4He reaction calculation with three-body Faddeev equations

International Nuclear Information System (INIS)

Oryu, S.; Uzu, E.; Sunahara, H.; Yamada, T.; Tabaru, G.; Hino, T.

1998-01-01

In order to investigate the 3 He-n-p system as a three-body problem, we have formulated 3 He-n and 3 H-p effective potentials using both a microscopic treatment and a phenomenological approach. In the microscopic treatment, potentials are generated by means of the resonating group method (RGM) based on the Minnesota nucleon-nucleon potential. These potentials are converted into separable form by means of the microscopic Pauli correct (MPC) method. The MPC potentials are properly formulated to avoid Pauli forbidden states. The phenomenological potentials are obtained by modifying parameters of the EST approximation to the Paris nucleon-nucleon potential, such that they fit the low-energy 3 He-n, 3 H-p, and 3 He-p phase shifts. Therefore, they describe the 3 He-n differential cross section, the polarization observables, and the energy levels of 4 He. The 3 He-n-p Faddeev equations are solved numerically. We reproduce correctly the ground state and the first excited state of 5 Li. Furthermore, the Paris-type potential is used to investigate the 3 He(d,p) 4 He reaction at a deuteron bombarding energy of 270 MeV, where the system is treated as a three-body problem. Results for the polarized and unpolarized differential cross sections demonstrate convergence of the Born series. (orig.)

Characterisation of a Personal TL Photon Dosemeter Based on Two LiF(Mg,Cu,P) Detectors in Terms of Hp(0.07,alpha) and Hp(10,alpha)

International Nuclear Information System (INIS)

Fantuzzi, E.; Monteventi, F.; Sermenghi, I.; Uleri, G.

1999-01-01

A study was undertaken to evaluate the response of the ENEA Photon Personal Dosemeter, slightly modified in design, in terms of H p (0.07, α) and H p (10, α). A filtration of 20 and 270 mg.cm -2 respectively for the two LiF(Mg,Cu,P) detectors employed was adopted according to a preliminary analytical simulation. The new dosemeter can measure both H p (0.07, α) with a maximum uncertainty of ±4% in the range from 10 to 200 keV and H p (10, α) with a maximum uncertainty of ±15% in the range from 10 keV to 1.25 MeV, using the detector responses together with a correction function with respect to their ratio. Moreover, tests were performed to evaluate the dosemeter angular response (0 deg., 20 deg., 40 deg., and 60 deg.) obtaining good results according to international recommendations. All irradiation tests were performed at SSL of ENEA Institute for Radiation Protection in Bologna using the ISO water phantom (30 x 30 x 15 cm 3 ) and standard ISO filtered X ray beams as well as 137 Cs and 60 Co sources. This work represents a preliminary type test of the ENEA photon personal dosemeter and allows its introduction in routine dosimetry practice in accordance with the ICRU reference quantities. (author)

The prognostic value of pimonidazole and tumour pO2 in human cervix carcinomas after radiation therapy: A prospective international multi-center study

International Nuclear Information System (INIS)

Nordsmark, Marianne; Loncaster, Julie; Aquino-Parsons, Christina; Chou, S.-C.; Gebski, Val; West, Catharine; Lindegaard, Jacob C.; Havsteen, Hanne; Davidson, Susan E.; Hunter, Robin; Raleigh, James A.; Overgaard, Jens

2006-01-01

Background and purpose: Hypoxia adversely affects treatment outcome in human uterine cervical cancer. Here, we present the results of a prospective international multi-centre study evaluating the prognostic value of pre-treatment tumour oxygen partial pressure (pO 2 ) and the hypoxia marker pimonidazole (pimo). Materials and methods: One hundred and twenty-seven patients with primary cervix cancer were entered. Pre-treatment tumour pO 2 measurements were obtained, and reported by the median tumour pO 2 , the fraction of pO 2 values ≤10 mmHg (HP 1 ), ≤5 mmHg (HP 5 ) and ≤2.5 mmHg (HP 2.5 ). Following intravenous pimonidazole administration, biopsies were taken, stained for pimonidazole adducts, and scored for the area of labelled tumour cells on a scale from 0 to 4. Treatment modalities were surgery (11%), radiotherapy (98%), chemotherapy (33%) and carbogen (14%). Results: None of the hypoxia descriptors were statistically significant prognostic factors for loco-regional tumour control or overall survival when analyzed as continuous variables or divided by the sample median. By univariate analysis only tumour size and nodal status were significant prognostic factors for local control. Tumour size and FIGO stage were significant for overall survival. In a multivariate analysis stratified by centre, only tumour size above 5 cm and lower pre-treatment haemoglobin predicted poorer overall survival among FIGO stage, nodal involvement, tumour size, pre-treatment haemoglobin dichotomized at 12 g/dl and pimo 1, pimo 4 and HP 5 as continuous variables. Conclusion: Neither Eppendorf nor pimonidazole should be dismissed based on the current results. However, further investigations are needed to readdress the hypotheses of the current study having optimized statistical designs, and a population of sufficient size treated more homogenously following rigorous protocols

Developmental validation of the Quantifiler(®) HP and Trio Kits for human DNA quantification in forensic samples.

Science.gov (United States)

Holt, Allison; Wootton, Sharon Chao; Mulero, Julio J; Brzoska, Pius M; Langit, Emanuel; Green, Robert L

2016-03-01

The quantification of human genomic DNA is a necessary first step in the DNA casework sample analysis workflow. DNA quantification determines optimal sample input amounts for subsequent STR (short tandem repeat) genotyping procedures, as well as being a useful screening tool to identify samples most likely to provide probative genotypic evidence. To better mesh with the capabilities of newest-generation STR analysis assays, the Quantifiler(®) HP and Quantifiler(®) Trio DNA Quantification Kits were designed for greater detection sensitivity and more robust performance with samples that contain PCR inhibitors or degraded DNA. The new DNA quantification kits use multiplex TaqMan(®) assay-based fluorescent probe technology to simultaneously quantify up to three human genomic targets, allowing samples to be assessed for total human DNA, male contributor (i.e., Y-chromosome) DNA, as well as a determination of DNA degradation state. The Quantifiler HP and Trio Kits use multiple-copy loci to allow for significantly improved sensitivity compared to earlier-generation kits that employ single-copy target loci. The kits' improved performance provides better predictive ability for results with downstream, newest-generation STR assays, and their shortened time-to-result allows more efficient integration into the forensic casework analysis workflow. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Liposome-encapsulated EF24-HP{beta}CD inclusion complex: a preformulation study and biodistribution in a rat model

Energy Technology Data Exchange (ETDEWEB)

Agashe, H; Lagisetty, P; Sahoo, K; Bourne, D [University of Oklahoma Health Sciences Center, Department of Pharmaceutical Sciences (United States); Grady, B [School of Chemical, Biological and Materials Engineering (United States); Awasthi, V [University of Oklahoma Health Sciences Center, Department of Pharmaceutical Sciences (United States)

2011-06-15

3,5-Bis(2-fluorobenzylidene)-4-piperidone (EF24) is an anti-proliferative diphenyldifluoroketone analog of curcumin with more potent activity. The authors describe a liposome preparation of EF24 using a 'drug-in-CD-in liposome' approach. An aqueous solution of EF24 and hydroxypropyl-{beta}-cyclodextrin (HP{beta}CD) inclusion complex (IC) was used to prepare EF24 liposomes. The liposome size was reduced by a combination of multiple freeze-thaw cycles. Co-encapsulation of glutathione inside the liposomes conferred them with the capability of labeling with imageable radionuclide Tc-99m. Phase solubility analysis of EF24-HP{beta}CD mixture provided k{sub 1:1} value of 9.9 M{sup -1}. The enhanced aqueous solubility of EF24 (from 1.64 to 13.8 mg/mL) due to the presence of HP{beta}CD helped in the liposome preparation. About 19% of the EF24 IC was encapsulated inside the liposomes (320.5 {+-} 2.6 nm) by dehydration-rehydration technique. With extrusion technique, the size of 177 {+-} 6.5 nm was obtained without any effect on encapsulation efficiency. The EF24-liposomes were evaluated for anti-proliferative activity in lung adenocarcinoma H441 and prostate cancer PC-3 cells. The EF24-liposomes demonstrated anti-proliferative activity superior to that of plain EF24 at 10 {mu}M dose. When injected in rats, the Tc-99m-labeled EF24-liposomes cleared from blood with an {alpha}-t{sub 1/2} of 21.4 min and {beta}-t{sub 1/2} of 397 min. Tissue radioactivity counting upon necropsy showed that the majority of clearance was due to the uptake in liver and spleen. The results suggest that using 'drug-in-CD-in liposome' approach is a feasible strategy to formulate an effective parenteral preparation of EF24. In vitro studies show that the liposomal EF24 remains anti-proliferative, while presenting an opportunity to image its biodistribution.

The intercomparison of 137Cs irradiator output measurement and personal dose equivalent, Hp(10), using TLD and film

International Nuclear Information System (INIS)

Nazaroh; Susetyo Trijoko; Sri Inang Sunaryati

2010-01-01

Intercomparison of output measurement of 137 Cs irradiator and personal dose equivalent, Hp(10) using TLD and film have been carried out in the year of 2006 to 2008. According to IAEA recommendation, intercomparison is one of audit activities but it is performed in the spirit of collaboration and support rather than in the spirit of inspection. The aim of intercomparison of output measurement of 137 Cs irradiator is to verify the dose stated by the participant laboratories. Intercomparison is also to assess the competency of the participant, to keep traceability and consistency of measurement result, to assure that instrument work correctly and the result of evaluation was in agreement, and also for fulfilling one of the clauses of ISO-17025-2005. Besides that, this intercomparison aimed to facilitate link between the system and members of national measurement and transfer of experience in measurement technique and dose evaluation of radiation. The benefit of intercomparison is important among others as tests of proficiency in dose evaluation or measurement, upgrading quality of service and for obeying supervisor body legislation (BAPETEN). TLD was used as a means of output 137 Cs irradiator measurement, whereas film and TLD were used for dose intercomparison. This paper presented result of intercomparison of output measurement and evaluation of personal dose equivalent, Hp(10) in the year of 2006 to 2008 followed by 6 participants: Balai Pengamanan Fasilitas Kesehatan (BPFK) Jakarta, Medan, Surabaya, Makasar, PTLR and Laboratory of Keselamatan dan Kesehatan Lingkungan (KKL)-PTKMR BATAN. In this intercomparison, the dose of TLD stated by participant were compared with the dose measured by Radiation Metrology Laboratory (LMR), and the results showed the differences were within 10 %, so it was satisfied. The results of intercomparison of personal dose equivalent, Hp(10) were evaluated based on ISO/IEC Guide 43-1, 1997 analysis and expressed as E n . The values of E n

Liposome-encapsulated EF24-HP{beta}CD inclusion complex: a preformulation study and biodistribution in a rat model

Energy Technology Data Exchange (ETDEWEB)

Agashe, H.; Lagisetty, P.; Sahoo, K.; Bourne, D. [University of Oklahoma Health Sciences Center, Department of Pharmaceutical Sciences (United States); Grady, B. [School of Chemical, Biological and Materials Engineering (United States); Awasthi, V., E-mail: vawasthi@ouhsc.edu [University of Oklahoma Health Sciences Center, Department of Pharmaceutical Sciences (United States)

2011-06-15

3,5-Bis(2-fluorobenzylidene)-4-piperidone (EF24) is an anti-proliferative diphenyldifluoroketone analog of curcumin with more potent activity. The authors describe a liposome preparation of EF24 using a 'drug-in-CD-in liposome' approach. An aqueous solution of EF24 and hydroxypropyl-{beta}-cyclodextrin (HP{beta}CD) inclusion complex (IC) was used to prepare EF24 liposomes. The liposome size was reduced by a combination of multiple freeze-thaw cycles. Co-encapsulation of glutathione inside the liposomes conferred them with the capability of labeling with imageable radionuclide Tc-99m. Phase solubility analysis of EF24-HP{beta}CD mixture provided k{sub 1:1} value of 9.9 M{sup -1}. The enhanced aqueous solubility of EF24 (from 1.64 to 13.8 mg/mL) due to the presence of HP{beta}CD helped in the liposome preparation. About 19% of the EF24 IC was encapsulated inside the liposomes (320.5 {+-} 2.6 nm) by dehydration-rehydration technique. With extrusion technique, the size of 177 {+-} 6.5 nm was obtained without any effect on encapsulation efficiency. The EF24-liposomes were evaluated for anti-proliferative activity in lung adenocarcinoma H441 and prostate cancer PC-3 cells. The EF24-liposomes demonstrated anti-proliferative activity superior to that of plain EF24 at 10 {mu}M dose. When injected in rats, the Tc-99m-labeled EF24-liposomes cleared from blood with an {alpha}-t{sub 1/2} of 21.4 min and {beta}-t{sub 1/2} of 397 min. Tissue radioactivity counting upon necropsy showed that the majority of clearance was due to the uptake in liver and spleen. The results suggest that using 'drug-in-CD-in liposome' approach is a feasible strategy to formulate an effective parenteral preparation of EF24. In vitro studies show that the liposomal EF24 remains anti-proliferative, while presenting an opportunity to image its biodistribution.

Timing and processes for exhumation of HP/LT rocks of the southern Brooks Range (AK): Insight from combined geochemistry and 40Ar/39Ar thermochronology of white mica

Science.gov (United States)

O'Brien, T.; Miller, E. L.; Grove, M. J.; Hayden, L. A.

2015-12-01

The obduction of an island arc onto the Arctic Alaska continental margin in the Jura-Cretaceous led to southward subduction of continental crust and high-pressure/low-temperature (HP/LT) epidote-blueschist facies metamorphism in the southern Brooks Range (BR). A regionally developed greenschist facies normal-sense shear zone system along the southern margin of the BR suggests that extensional faulting had an influential role in the exhumation of the metamorphic core. To better constrain the exhumation history of the metamorphic core of the BR, samples were collected from a N-S transect through the metamorphic core of the orogen. Electron microprobe (EMP) analyses of white micas reveal that they are composed of phengite (Si > 3.0 pfu) or a combination of phengite + paragonite. Si-content of phengites reveal a southward increase in Si from 3.1 to 3.4 pfu (corresponding to an increase in pressure). Si-contents in higher-P phengites are scattered, reflecting subsequent muscovite growth. The Si trend is matched by a southward increase in the 40Ar/39Ar total gas ages of white micas. Phengite from the north are characterized by younger ages (~115 Ma) and flatter 40Ar/39Ar spectra. Farther south, phengites and paragonites yield older 40Ar/39Ar ages. These samples yield convex, staircase 40Ar/39Ar spectra that initiate ~115-120 Ma and flatten-out ~130-138 Ma. Modeling using MacArgon proposes that an initial cooling of HP/LT metamorphism occurred ~130-138 Ma, recorded in the high-Si phengites and paragonites. Following initial cooling, modeling suggests HP/LT rocks stalled in the greenschist facies field before subsequent exhumation, resulting in the staircase 40Ar/39Ar spectra. Flatter 40Ar/39Ar spectra recorded by the northern samples and modeling of 40Ar/39Ar results from the southern samples suggest that these rocks from metamorphic core of the BR were exhumed to temperatures < 300°C by ~115 Ma.

Simulation and thermoeconomic analysis of different configurations of gas turbine (GT)-based dual-purpose power and desalination plants (DPPDP) and hybrid plants (HP)

International Nuclear Information System (INIS)

Rensonnet, Thibaut; Uche, Javier; Serra, Luis

2007-01-01

This paper contains a simulation and a thermoeconomic analysis of several configurations of gas turbine (GT)-based dual-purpose power and desalination plants (DPPDP): Gas turbine with reverse osmosis (GT+RO), combined cycle with reverse osmosis (CC+RO), combined cycle with multi-effect distillation (CC+MED) and two different hybrid plant (HP) arrangements combining CC, MED and RO (CC+MED+RO, CC+MED+RO bis ). The last two configurations only differ from the feed solution to the MED units (raw seawater or brine coming from the RO discharge). A complete thermodynamic simulation at both design and at part load conditions has been made, as well as an exergy and an exergo-economic (thermoeconomic) analysis of each configuration, in order to compare the evolution of the water and electricity cost for different arrangements. The results show that even for a significantly reduced fuel cost (1.42 $/GJ), the CC is much more profitable than a GT operating in open cycle, with electricity cost values of 1.647 and 2.166 c$/kWh, respectively. As was expected, RO is more efficient and profitable than MED desalination processes, the difference in the obtained desalted water cost being significant. In the hybrid configuration with MED fed by the RO brine discharge, a decrease in the equivalent electrical consumption of nearly 2 kWh/m 3 was achieved, but even in this case RO was more efficient (14.15 vs. 4.048 kWh/m 3 ). The evolution of electricity cost in each configuration is more similar at part load operation than at full load, but in the case of water cost, RO is once again more profitable and less sensitive to load variations. Costs given in this paper correspond to investment and fuel costs. Further, profitability and operation strategies of HP, i.e., DPPDP combining distillation and membrane processes, are also analyzed. It is shown that HP can be more profitable than RO plants in the case of increasing the water production capacity of existing DPPDP, because the profit

Validación de una escala para medir la calidad de vida laboral en hospitales públicos de Tlaxcala

Directory of Open Access Journals (Sweden)

Irma Alejandra Hernández-Vicente

2017-03-01

Full Text Available Objetivo. Validar un instrumento para medir la calidad de vida laboral en hospitales públicos (CVL-HP de Tlaxcala,México. Material y métodos. El instrumento fue validado en 669 trabajadores de seis hospitales de la Secretaría de Salud de Tlaxcala, México. Se evaluó validez de contenido por consulta a expertos, de constructo mediante análisis factorial, de criterio por comparación con otras escalas y la confiabilidad con Alpha de Cronbach. Resultados. El análisis factorial descubrió cuatro dimensiones: “bienestar individual”,“condiciones y medio ambiente de trabajo”, “organización”y “bienestar logrado a través del trabajo”. La confiabilidad fue de 0.921. Los trabajadores con mejor CVL-HP fueron menores de 50 años, de contrato, con menor antigüedad laboral, personal de jornada acumulada diurna y aquéllos con licenciatura. Conclusiones. La escala CVL-HP mostró ser psicométricamente válida y confiable. Se recomienda probar esta escala en otras instituciones públicas y privadas,y relacionarla con  indicadores de desempeño y gestión de los servicios de salud.

Determination of the dose equivalent Hp(0.07) in hands of occupationally exposed personnel in the practice of proton emission tomography (PET/CT); Determinacion de la dosis equivalente Hp(0.07) en manos de trabajadores ocupacionalmente expuestos en la practica de Tomografia por Emision de Positrones (PET/CT)

Energy Technology Data Exchange (ETDEWEB)

Lea, D. [Servicio de Radiofisica Sanitaria, Unidad de Tecnologia Nuclear, Instituto Venezolano de Investigaciones Cientificas, Ministerio de Ciencia y Tecnologia, Km 11 Carretera Panamerican, Altos del Pipe, Caracas (Venezuela); Ruiz, N.; Esteves, L. [Centro Diagnostico Docente Las Mercedes, Calle Paris cruce con calle Caroni, Edif. CDD, Las Mercedes, Caracas (Venezuela)]. e-mail: dlea@ivic.ve

2006-07-01

In Venezuela recently it was implanted the Positron Emission Tomography technique (PET) with the perspective of implanting it at national level. Even when in our country practices it of nuclear medicine it exists from early of 70, there is not experience in the determination of the occupational doses by exposure to the external radiation in hands. By this reason, a concern exists in the workers of the centers of nuclear medicine where it is practiced the Positron Emission Tomography technique. In absence of the TLD dosimetry to measure dose in hands in our country, measurements of the dose equivalent of the workers of the PET national reference center were made, using a detector of hands type diode. It was determined the dose in hands in terms of dose equivalent Hp(0.07) in two work positions, that is: the corresponding to the transfer of the receiving vial of ({sup 18}F) FDG to the shield, quality control and uni doses division. The second work position corresponds the person in charge of administering, via intravenous, the ({sup 18}F) FDG. In this work it realizes the dose equivalent in hands Hp(0.07) measures in each one of the work positions before described by daily production. The informed doses correspond to a total average produced activity of 20.4 GBq (550 mCi). The results of the measurements in terms of dose equivalent in hands Hp(0.07) correspond to 2.1 {+-} 20% mSv in the work position of division and 0.4 {+-} 10% mSv in the position of injection of the radioactive material. At short term this foreseen until 4 productions per week, what means an annual dose equivalent Hp(0.07) in hands of 400 mSv approximately, without taking into account abnormal situations as its are spills of the ({sup 18}F) FDG in the work place. This work is the starting point so that the regulatory authority settles down, in Venezuela, dose restrictions in the PET practices and implant, in the centers of nuclear medicine, an optimization politics of this practice in conformity

Analyzing powers in the dd→3Hen(3Hp) reactions at intermediate energies

International Nuclear Information System (INIS)

Ladygin, V. P.; Kiselev, A. S.; Kurilkin, A. K.; Vasiliev, T. A.; Isupov, A. Yu.; Ladygina, N. B.; Malakhov, A. I.; Reznikov, S. G.; Uesaka, T.; Saito, T.; Hatano, M.; Kato, H.; Sakoda, S.; Uchigashima, N.; Yako, K.; Janek, M.; Maeda, Y.; Nishikawa, J.; Ohnishi, T.; Sakamoto, N.

2008-01-01

Data for the deuteron analyzing powers in the dd→ 3 Hen( 3 Hp) reactions obtained at 140-270 MeV are discussed. The observed negative sign of the tensor analyzing powers A yy , A xx and A xz at small angles clearly demonstrate the sensitivity to the ratio of the D and S state components of the 3 He wave function. The behavior of the tensor analyzing powers at backward angles is sensitive to the short-range spin structure of the deuteron. However, the one-nucleon exchange calculations using standard 3 He and deuteron wave functions fail to reproduce the strong variation of the tensor analyzing powers as a function of angle in the cms. Sensitivity to relativistic effects is also discussed

Beneficial effects of hydroxyapatite on enamel subjected to 30% hydrogen peroxide.

Science.gov (United States)

Jiang, Tao; Ma, Xiao; Wang, Zhejun; Tong, Hua; Hu, Jiming; Wang, Yining

2008-11-01

To evaluate the effect of combination of hydroxyapatite (HA) and hydrogen peroxide (HP) on color, microhardness and morphology of human tooth enamel. Forty-eight human dental blocks were obtained from 12 pairs of premolars and were randomly divided into four groups. Group DW was treated with distilled water, group HP with 30% HP, group HA+DW with HA mixed with distilled water and group HA+HP with HA mixed with 30% HP. Baseline and final color measurements and microhardness test were carried out before and after bleaching experiments. Two specimens from each group were selected for morphological investigation after final tests. The DeltaE of group HP and HA+HP were significantly higher than those of group DW (p=0.000 and p=0.000) and group HA+DW (p=0.000 and p=0.000). The percentage microhardness loss of group HA+HP was significantly lower than that of group HP (p=0.047), but significantly higher than those of group DW (p=0.000) and group HA+DW (p=0.000). The obvious variation of morphology was only observed on enamel surfaces in group HP. This study suggested that combination of HA and HP was effective in tooth whitening. HA could significantly reduce the microhardness loss of enamel caused by 30% HP and keep enamel surface morphology almost unchanged.

Comparison of multimesh hp-FEM to interpolation and projection methods for spatial coupling of thermal and neutron diffusion calculations

International Nuclear Information System (INIS)

Dubcova, Lenka; Solin, Pavel; Hansen, Glen; Park, HyeongKae

2011-01-01

Multiphysics solution challenges are legion within the field of nuclear reactor design and analysis. One major issue concerns the coupling between heat and neutron flow (neutronics) within the reactor assembly. These phenomena are usually very tightly interdependent, as large amounts of heat are quickly produced with an increase in fission events within the fuel, which raises the temperature that affects the neutron cross section of the fuel. Furthermore, there typically is a large diversity of time and spatial scales between mathematical models of heat and neutronics. Indeed, the different spatial resolution requirements often lead to the use of very different meshes for the two phenomena. As the equations are coupled, one must take care in exchanging solution data between them, or significant error can be introduced into the coupled problem. We propose a novel approach to the discretization of the coupled problem on different meshes based on an adaptive multimesh higher-order finite element method (hp-FEM), and compare it to popular interpolation and projection methods. We show that the multimesh hp-FEM method is significantly more accurate than the interpolation and projection approaches considered in this study.

Lactobacillus fermentum HP3-Mediated Fermented Hericium erinaceus Juice as a Health Promoting Food Supplement to Manage Diabetes Mellitus.

Science.gov (United States)

Chaiyasut, Chaiyavat; Woraharn, Sasimar; Sivamaruthi, Bhagavathi Sundaram; Lailerd, Narissara; Kesika, Periyanaina; Peerajan, Sartjin

2018-01-01

The current study investigated the antidiabetic property of Lactobacillus fermentum HP3-mediated fermented Hericium erinaceus juice (FHJ) using male Wistar rats with streptozotocin-induced diabetes mellitus (DM). FHJ was prepared using boiled mushroom juice and L. fermentum HP3. Amino acid and γ-aminobutyric acid (GABA) content of FHJ was analyzed. Streptozotocin-induced DM rats were supplemented with FHJ in a pre- and posttreatment method. The changes in plasma insulin, plasma glucose level, glycated hemoglobin (HbA1c), representative cytokines, and the antioxidant system were assessed in experimental rats using spectrophotometric methods and enzyme-linked immunosorbent assay. The supplementation of FHJ improved the body mass, insulin level, and recovery progress of hyperglycemia. HbA1c level was altered by the FHJ intervention. The inflammatory cytokines level was suppressed in FHJ supplemented group compared with control. Intervention of FHJ and insulin improved the production of interleukin-10 and transforming growth factor--β1 in DM rat. The study suggested that fermented H erinaceus juice may be used as one of the food-based health-promoting supplement to manage DM along with medication.

Evaluation of personal dose equivalent 'HP(d)' in a external individual monitoring system for X and gamma radiation

International Nuclear Information System (INIS)

Santoro, C.; Antonio Filho, J.; Santos, M.A.P.

2007-01-01

The good of individual monitoring for external radiation is the assessment of occupational exposure from X and γ radiations in order to assure that the radiological conditions of the workplace are acceptable, safe and satisfactory. The evaluation of radiations doses for workers must not exceed dose limits specified for workers, according to national regulatory agencies. Nowadays, there are two external monitoring systems in use, both based on ICRU definitions. In the conventional system, the workers doses are evaluated in terms of Hx. The personal dosimeter is worn over chest surface and it is calibrated in function of air kerma. In the new system, the workers doses are evaluated in terms of HP(d) and the personal dosimeter is calibrated in function of phantom doses. The aim of this paper is to adapt an external dosimetry laboratory (based on photographic dosimetry) to evaluate the personal dosimeters in terms of HP(d). In this way, a simple methodology, based on linear programming, was utilized. In this adaptation, calibration curves were obtained for radiation qualities (W and N series) described by International Organization for Standardization (ISO 4037-1, 1995). These calibration curves offer a better accuracy on dose determinations and energy below 140 keV, improving the quality of service rendered the society. (author)

Analysis of Gastric Body Microbiota by Pyrosequencing: Possible Role of Bacteria Other Than Helicobacter pylori in the Gastric Carcinogenesis.

Science.gov (United States)

Sohn, Sung-Hwa; Kim, Nayoung; Jo, Hyun Jin; Kim, Jaeyeon; Park, Ji Hyun; Nam, Ryoung Hee; Seok, Yeong-Jae; Kim, Yeon-Ran; Lee, Dong Ho

2017-06-01

Gastric microbiota along with Helicobacter pylori (HP) plays a key role in gastric disease. The aim of our study is to investigate the difference of human gastric microbiota between antrum and body according to disease (control vs. gastric cancer) and HP status. Each antrum and body biopsy was collected from 12 subjects at Seoul National University Bundang Hospital. Gastric microbiota was analyzed by bar-coded 454 pyrosequencing of the 16S rRNA gene. Twelve subjects consisted of HP-negative control (n = 2), HP-negative cancer (n = 2), HP-positive control (n = 3), and HP-positive cancer (n = 5). The analysis was focused on non-HP urease-producing bacteria (UB) and non-HP nitrosating or nitroreducing bacteria (NB) between antrum and body. Gastric body samples showed higher diversity compared to gastric antrum mucosa samples but there was no significant difference. The mean of operational taxonomic units was higher in HP(-) cancer than HP(+) cancer (antrum, 273.5 vs. 228.2, P = 0.439; body, 585.5 vs. 183.2, P = 0.053). The number of non-HP UB and non-HP NB was higher in HP(-) cancer groups than the others. These differences were more pronounced in the body ( P = 0.051 and P = 0.081, respectively). Analysis of overlap of non-HP UB and non-HP NB revealed the higher composition of Streptococcus pseudopneumoniae, S. parasanguinis , and S. oralis in HP(-) cancer groups than the others, only in the body ( P = 0.030) but not in the antrum ( P = 0.123). Higher diversity and higher composition of S. pseudopneumoniae, S. parasanguinis , and S. oralis in HP(-) cancer group than the other groups in the body suggest that analysis of microbiota from body mucosa could be beneficial to identify a role of non-HP bacteria in the gastric carcinogenesis.

Distinct Clinicopathological Features and Prognosis of Helicobacter pylori Negative Gastric Cancer.

Directory of Open Access Journals (Sweden)

Kun-Feng Tsai

Full Text Available Whether the characteristics and prognosis of gastric cancer (GC are different in patients with and without Helicobacter pylori (HP remains controversial. The definitions of HP status in patients with atrophic gastritis but negative tests for HP are heterogeneous. We aimed to assess the impact of HP on the prognosis of GC using different definitions.From 1998 Nov to 2011 Jul, five hundred and sixty-seven consecutive patients with GC were included. HP status was determined by serology and histology. Patients with any positive test were defined as HP infection. Patients without HP infection whose serum pepsinogen (PG I <70 ng/dl and PG I/II ratio < 3.0 were defined as atrophic gastritis and they were categorized into model 1: HP positive; model 2: HP negative; and model 3: exclusion of these patients.We found four characteristics of HP negative GC in comparison to HP positive GC: (1 higher proportion of the proximal tumor location (24.0%, P = 0.004, (2 more diffuse histologic type (56.1%, p = 0.008, (3 younger disease onset (58.02 years, p = 0.008 and (4 more stage IV disease (40.6%, p = 0.03. Patients with negative HP had worse overall survival (24.0% vs. 35.8%, p = 0.035. In Cox regression models, the negative HP status is an independent poor prognostic factor (HR: 1.34, CI:1.04-1.71, p = 0.019 in model 1, especially in stage I, II and III patients (HR: 1.62; CI:1.05-2.51,p = 0.026.We found the distinct characteristics of HP negative GC. The prognosis of HP negative GC was poor.

Efeitos do dimetilsulfóxido no estresse oxidativo e na regeneração hepática pós-hepatectomia em ratos

Directory of Open Access Journals (Sweden)

José Ulisses de Souza Melo

Full Text Available OBJETIVO: avaliar a influência do DMSO sobre o estresse oxidativo e a regeneração hepática pós-HP via um modelo experimental. MÉTODO: 36 ratos Wistar machos jovens foram aleatoriamente distribuídos em dois Grupos de 18 animais: parcialmente hepatectomizados com infusão diária de solução salina (controle e parcialmente hepatectomizados com aporte diário intraperitoneal de DMSO, todos por duas semanas. Nos tempos 36h (T1, 168h (T2 e 336h (T3 pós-HP, glutationa (GSH foi medida no plasma e no tecido hepático, enquanto glicose e bilirrubina total foram aquilatados no sangue. A massa do fígado residual, nos mesmos tempos, foi o parâmetro utilizado para estimar a evolução da regeneração do fígado. RESULTADOS: DMSO baixou os níveis de GSH hepático e sangüíneo mas não interferiu na evolução da massa em regeneração. CONCLUSÃO: DMSO inibiu o estresse oxidativo pós-HP mas não mostrou alterações significantes na regeneração hepática em ratos.

Tensor analyzing power T20 of the dd →3Hen and dd →3Hp reactions at zero angle for energies 140, 200, and 270 MeV

International Nuclear Information System (INIS)

Ladygin, V. P.; Uesaka, T.; Saito, T.; Hatano, M.; Isupov, A. Yu.; Kato, H.; Ladygina, N. B.; Maeda, Y.; Malakhov, A. I.; Nishikawa, J.; Ohnishi, T.; Okamura, H.; Reznikov, S. G.; Sakai, H.; Sakamoto, N.; Sakoda, S.; Satou, Y.; Sekiguchi, K.; Suda, K.; Tamii, A.

2006-01-01

RIKEN Accelerator Research Facility data on the tensor analyzing power T 20 of the dd → 3 Hen and dd → 3 Hp reactions at zero angle for deuteron kinetic energies of 140, 200, and 270 MeV are reported. The observed positive sign of T 20 clearly demonstrates the sensitivity to the D/S-wave ratio in the 3 He and 3 H wave functions in the energy range of the experiment. Data on T 20 for the 3 Hen channel are in agreement with those for the 3 Hp channel within the experimental errors

Crystallography and Morphology of Niobium Carbide in As-Cast HP-Niobium Reformer Tubes

Science.gov (United States)

Buchanan, Karl G.; Kral, Milo V.

2012-06-01

The microstructures of two as-cast heats of niobium-modified HP stainless steels were characterized. Particular attention was paid to the interdendritic niobium-rich carbides formed during solidification of these alloys. At low magnifications, these precipitates are grouped in colonies of similar lamellae. Higher magnifications revealed that the lamellae actually obtain two distinct morphologies. The type I morphology exhibits broad planar interfaces with a smooth platelike shape. Type II lamellae have undulating interfaces and an overall reticulated shape. To provide further insight into the origin of these two different morphologies, the microstructure and crystallography of each have been studied in detail using high resolution scanning electron microscopy, transmission electron microscopy, various electron diffraction methods (electron backscatter diffraction (EBSD), selected area diffraction (SAD), and convergent beam electron diffraction (CBED)), and energy dispersive X-ray spectroscopy.

Programs for data processing in radioimmunoassay using the HP-41C programmable calculator

International Nuclear Information System (INIS)

1981-09-01

The programs described provide for analysis, with the Hewlett Packard HP-41C calculator, of counting data collected in radioimmunoassays or other related in-vitro assays. The immediate reason for their development was to assist laboratories having limited financial resources and serious problems of quality control. The programs are structured both for ''off-line'' use, with manual entry of counting data into the calculator through the keyboard, and, in a slightly altered version, for ''on-line'' use, with automatic data entry from an automatic well scintillation counter originally designed at the IAEA. Only the off-line variant of the programs is described. The programs determine from appropriate counting data the concentration of analyte in unknown specimens, and provide supplementary information about the reliability of these results and the consistency of current and past assay performance

Fate of Subducting Organic Carbon: Evidence from HP/UHP Metasedimentary Suites

Science.gov (United States)

Kraft, K.; Bebout, G. E.

2017-12-01

Community interest in deep-Earth C cycling has focused attention on extents of C release from subducting oceanic lithosphere and sediment and the fate of this released C. Many have suggested that, based on isotopic and other arguments, 20% of the C subducted into the deeper mantle is in reduced form (organic); however, individual margins show large variation in carbonate to organic C ratios. Despite the size of the potentially deeply subducted organic C reservoir, its fate in subducting sections remains largely unexplored, with most attention paid to release of carbonate C. To characterize the forearc behavior of organic C, metamorphosed to P-T as high as that beneath volcanic fronts, we evaluated records of reduced C (RC) contents and isotope compositions in HP/UHP metasediments: 1) Schistes Lustres/Cignana (SLC) suite (Alps; Cook-Kollars et al., 2014, Chem Geol) with abundant carbonate and resembling sediment entering the East Sunda trench; and (2) Franciscan Complex (FC), W. Baja Terrain (WBT), Catalina Schist (CS) metasediments (Sadofsky and Bebout, 2003, G3), largely sandstone-shale sequences containing very little carbonate. In general, more Al-rich samples (shaley) in the terrigenous metasedimentary suites have higher concentrations of RC, which in low-grade units preserves δ13C of its organic protoliths. Carbonate-poor rocks in the SLC suite, and at ODP Site 765, show correlated major element (Al, Mg, Mn, Ti, P) and RC contents (up to 1.2 wt.%) reflecting sandstone-shale mixture. In the FC, WBT, and CS, the more Al-rich samples contain up to 2 wt. % RC. In high-grade Catalina Schist, RC has elevated δ13C due to C loss in CH4 and high-grade Alps rocks show reduced RC wt. % normalized to Al content. We consider processes that could alter contents and isotopic compositions of RC in sediment, e.g., devolatilization, closed-system exchange with carbonate, redox reactions, isotopic exchange with C in externally-derived fluids. It appears that, on modern Earth

Determination of the dose equivalent Hp(0.07) in hands of occupationally exposed personnel in the practice of proton emission tomography (PET/CT)

International Nuclear Information System (INIS)

Lea, D.; Ruiz, N.; Esteves, L.

2006-01-01

In Venezuela recently it was implanted the Positron Emission Tomography technique (PET) with the perspective of implanting it at national level. Even when in our country practices it of nuclear medicine it exists from early of 70, there is not experience in the determination of the occupational doses by exposure to the external radiation in hands. By this reason, a concern exists in the workers of the centers of nuclear medicine where it is practiced the Positron Emission Tomography technique. In absence of the TLD dosimetry to measure dose in hands in our country, measurements of the dose equivalent of the workers of the PET national reference center were made, using a detector of hands type diode. It was determined the dose in hands in terms of dose equivalent Hp(0.07) in two work positions, that is: the corresponding to the transfer of the receiving vial of ( 18 F) FDG to the shield, quality control and uni doses division. The second work position corresponds the person in charge of administering, via intravenous, the ( 18 F) FDG. In this work it realizes the dose equivalent in hands Hp(0.07) measures in each one of the work positions before described by daily production. The informed doses correspond to a total average produced activity of 20.4 GBq (550 mCi). The results of the measurements in terms of dose equivalent in hands Hp(0.07) correspond to 2.1 ± 20% mSv in the work position of division and 0.4 ± 10% mSv in the position of injection of the radioactive material. At short term this foreseen until 4 productions per week, what means an annual dose equivalent Hp(0.07) in hands of 400 mSv approximately, without taking into account abnormal situations as its are spills of the ( 18 F) FDG in the work place. This work is the starting point so that the regulatory authority settles down, in Venezuela, dose restrictions in the PET practices and implant, in the centers of nuclear medicine, an optimization politics of this practice in conformity with the ALARA

HP-Multigrid as Smoother algorithm for higher order discontinuous Galerkin discretizations of advection dominated flows. Part II: Optimization of the Runge-Kutta smoother

NARCIS (Netherlands)

van der Vegt, Jacobus J.W.; Rhebergen, Sander

2012-01-01

Using a detailed multilevel analysis of the complete hp-Multigrid as Smoother algorithm accurate predictions are obtained of the spectral radius and operator norms of the multigrid error transformation operator. This multilevel analysis is used to optimize the coefficients in the semi-implicit

The Drosophila melanogaster CHD1 chromatin remodeling factor modulates global chromosome structure and counteracts HP1a and H3K9me2.

Science.gov (United States)

Bugga, Lakshmi; McDaniel, Ivy E; Engie, Liana; Armstrong, Jennifer A

2013-01-01

CHD1 is a conserved chromatin remodeling factor that localizes to active genes and functions in nucleosome assembly and positioning as well as histone turnover. Mouse CHD1 is required for the maintenance of stem cell pluripotency while human CHD1 may function as a tumor suppressor. To investigate the action of CHD1 on higher order chromatin structure in differentiated cells, we examined the consequences of loss of CHD1 and over-expression of CHD1 on polytene chromosomes from salivary glands of third instar Drosophila melanogaster larvae. We observed that chromosome structure is sensitive to the amount of this remodeler. Loss of CHD1 resulted in alterations of chromosome structure and an increase in the heterochromatin protein HP1a, while over-expression of CHD1 disrupted higher order chromatin structure and caused a decrease in levels of HP1a. Over-expression of an ATPase inactive form of CHD1 did not result in severe chromosomal defects, suggesting that the ATPase activity is required for this in vivo phenotype. Interestingly, changes in CHD1 protein levels did not correlate with changes in the levels of the euchromatin mark H3K4me3 or elongating RNA Polymerase II. Thus, while CHD1 is localized to transcriptionally active regions of the genome, it can function to alter the levels of HP1a, perhaps through changes in methylation of H3K9.

The Drosophila melanogaster CHD1 chromatin remodeling factor modulates global chromosome structure and counteracts HP1a and H3K9me2.

Directory of Open Access Journals (Sweden)

Lakshmi Bugga

Full Text Available CHD1 is a conserved chromatin remodeling factor that localizes to active genes and functions in nucleosome assembly and positioning as well as histone turnover. Mouse CHD1 is required for the maintenance of stem cell pluripotency while human CHD1 may function as a tumor suppressor. To investigate the action of CHD1 on higher order chromatin structure in differentiated cells, we examined the consequences of loss of CHD1 and over-expression of CHD1 on polytene chromosomes from salivary glands of third instar Drosophila melanogaster larvae. We observed that chromosome structure is sensitive to the amount of this remodeler. Loss of CHD1 resulted in alterations of chromosome structure and an increase in the heterochromatin protein HP1a, while over-expression of CHD1 disrupted higher order chromatin structure and caused a decrease in levels of HP1a. Over-expression of an ATPase inactive form of CHD1 did not result in severe chromosomal defects, suggesting that the ATPase activity is required for this in vivo phenotype. Interestingly, changes in CHD1 protein levels did not correlate with changes in the levels of the euchromatin mark H3K4me3 or elongating RNA Polymerase II. Thus, while CHD1 is localized to transcriptionally active regions of the genome, it can function to alter the levels of HP1a, perhaps through changes in methylation of H3K9.

The tensor analyzing power T20 in the dd → 3Hen and dd → 3Hp reactions at the energies 140, 200 and 270 MeV at zero angle

International Nuclear Information System (INIS)

Ladygin, V.P.; ); Uesaka, T.; Saito, T.

2006-01-01

The data on the tensor analyzing power T 20 in the dd → 3 Hen and dd → 3 Hp reactions at 140, 200 and 270 MeV of the deuteron kinetic energy and at zero angle obtained at RIKEN Accelerator Research Facility are presented. The observed positive sign of T 20 clearly demonstrates the sensitivity to the D/S wave ratios in the 3 He and 3 H in the energy domain of the measurements. The T 20 data for the 3 Hen and 3 Hp channels are in agreement within experimental accuracy [ru

Occurrence of tephra/volcanic tuff in the tertiary sediments of Himachal Himalaya from Tileli area, Mandi district, H.P.: implication for stratigraphy and uranium mineralization

International Nuclear Information System (INIS)

Pandey, Pradeep; Chabbra, Jyotsana; Joshi, G.B.; Parihar, P.S.

2014-01-01

Presence of Early Tertiary pyroclastic material (tephra) has been documented petrographically, for the first time, in the Mandi-Bilaspur Sector from Tileli area, Dharamsala basin of Himachal Pradesh. The tephra is reported from the red shale, identified as tuffaceous siltstone belonging to lower Dharamsala Formation that lies above the uraniferous sandstone body and occurs as thin layers of over 300m along the strike, close to the contact of lower and upper Dharamsala formations. The tuffaceous material shows crude but preferred orientation of minerals like biotite, muscovite, chlorite, clay, hematite and specularite. Various features indicating presence of tephra are, glass shards altered to clay but retaining 'U' shaped outline, spindle-shaped hematite with preferred orientation, spherical to sub-spherical clay and altered Fe oxide rich balls, clay groundmass with flow pattern, flaky minerals in association with clast depicting asymmetrical ramp structure. A zone of approximately 300 m length containing tuffaceous material has been established at Tileli overlying the uraniferous sandstone body. Identification of tephra at Tileli has significant implications as it enabled in demarcating the boundary between the upper and lower Dharamsala formations in central part of the basin in Bilaspur-Mandi Sector of HP Himalaya and also in guiding the uranium exploration programme in the lower Dharamsala Formation. (author)

Execution of a cooperative test by means of 'in-situ-gamma spectrometry' using HP-germanium detectors

International Nuclear Information System (INIS)

Steger, F.; Lovranich, E.; Urbanich, E.; Streit, S.

1995-06-01

A cooperative test was carried out in Salzburg, Austria, in order to determine the activity of Cs-137 and other radio nuclides from the Tschernobyl fallout 1986 and from tests of nuclear weapons in the 1960s. 24 groups from 9 countries of Europe took part. The measurements were performed by means of gamma spectrometry using HP-germanium detectors. Most of the groups had excellent results. The groups became acquainted with each other. The personal contact makes it possible to get information quickly in case of accidents of nuclear power stations in the neighbourhood of Austria

Lactobacillus fermentum HP3–Mediated Fermented Hericium erinaceus Juice as a Health Promoting Food Supplement to Manage Diabetes Mellitus

Science.gov (United States)

Chaiyasut, Chaiyavat; Woraharn, Sasimar; Sivamaruthi, Bhagavathi Sundaram; Lailerd, Narissara; Kesika, Periyanaina; Peerajan, Sartjin

2018-01-01

The current study investigated the antidiabetic property of Lactobacillus fermentum HP3–mediated fermented Hericium erinaceus juice (FHJ) using male Wistar rats with streptozotocin-induced diabetes mellitus (DM). FHJ was prepared using boiled mushroom juice and L. fermentum HP3. Amino acid and γ-aminobutyric acid (GABA) content of FHJ was analyzed. Streptozotocin-induced DM rats were supplemented with FHJ in a pre- and posttreatment method. The changes in plasma insulin, plasma glucose level, glycated hemoglobin (HbA1c), representative cytokines, and the antioxidant system were assessed in experimental rats using spectrophotometric methods and enzyme-linked immunosorbent assay. The supplementation of FHJ improved the body mass, insulin level, and recovery progress of hyperglycemia. HbA1c level was altered by the FHJ intervention. The inflammatory cytokines level was suppressed in FHJ supplemented group compared with control. Intervention of FHJ and insulin improved the production of interleukin-10 and transforming growth factor-–β1 in DM rat. The study suggested that fermented H erinaceus juice may be used as one of the food-based health-promoting supplement to manage DM along with medication. PMID:29619846

pH and effects on Streptococcus mutans growth of denture adhesives: an in vitro study.

Science.gov (United States)

Chen, Fengying; Mao, Tiantian; Cheng, Xiangrong

2014-06-01

To evaluate the pH and effects on Streptococcus mutans growth of denture adhesives. There is little information regarding the pH of contemporary adhesives and their influences on S. mutans growth. The adhesives tested were Polident® cream, Protefix® cream and Protefix® powder. Samples of each adhesive were added to deionized water to produce solutions of 10.0, 5.0, 2.5 and 1.0% w/v (cream formulations) or 5.0, 2.5,1.0 and 0.5% (powder formulation). The pH values were measured immediately after preparation and at 1-, 2-, 3-, 6-, 12-, and 24-h intervals using a digital pH meter. Streptococcus mutans UA159 was inoculated in the Brain Heart Infusion medium with or without the adhesive extracts (control). Bacterial growth was observed by measuring absorption at 600 nm every 1 h for 12 h using a spectrophotometer. The tested adhesives generally remained relatively pH-stable over 24 h, ranging from 5.5 to 7.0. There were no statistically significant differences in S. mutans growth rates between the extract-treated and control cultures (p>0.5). Some adhesives produce a pH below the critical pH of hydroxyapatite and may not be suitable for patients with natural teeth. None of the tested adhesives significantly affect S. mutans growth. © 2012 The Gerodontology Society and John Wiley & Sons A/S.

Fate and transport of mercury in soil systems : a numerical model in HP1 and sensitivity analysis

Science.gov (United States)

Leterme, Bertrand; Jacques, Diederik

2013-04-01

speciation data on a contaminated sites would be required, together with a good characterization of the pollution source. References : Blanc, P., Lassin, A. and Piantone, P. (2012), THERMODDEM a database devoted to waste minerals, BRGM, Orléans, France. http://thermoddem.brgm.fr Campolongo, F., Cariboni, J. and Saltelli, A. (2007), An effective screening design for sensitivity analysis of large models, Environmental Modelling & Software 22(10): 1509-1518. Jacques, D. and Šimůnek, J. (2010), Notes on HP1 - a software package for simulating variably-saturated water flow, heat transport, solute transport and biogeochemistry in porous media, HP1 Version 2.2 SCK•CEN-BLG-1068, Waste & Disposal Department, SCK•CEN, Mol, Belgium: 113 p. Morris, M. D. (1991), Factorial Sampling Plans for Preliminary Computational Experiments, Technometrics 33(2): 161-174. Skyllberg, U. (2012), Chemical Speciation of Mercury in Soil and Sediment. Environmental Chemistry and Toxicology of Mercury, John Wiley & Sons, Inc.: 219-258.

Effect of pH on the complexation of kaempferol-4'-glucoside with three β-cyclodextrin derivatives: isothermal titration calorimetry and spectroscopy study.

Science.gov (United States)

Zheng, Yan; Dong, Li-Na; Liu, Min; Chen, Aiju; Feng, Shangcai; Wang, Bingquan; Sun, Dezhi

2014-01-08

The utilization of kaempferol and its glycosides in food and pharmaceutical industries could be improved by the formation of inclusion complexes with cyclodextrins at different pH. This study explores the complexation of kaempferol-4'-glucoside with sulfobutyl ether-β-cyclodextrin (SBE-β-CD), hydroxypropyl-β-cyclodextrin (HP-β-CD), and methylated-β-cyclodextrin (M-β-CD) in phosphate buffer solutions of different pH using isothermal titration calorimetry, UV-vis absorption and proton nuclear magnetic resonance spectroscopy at 298.2 K. Experimental results showed that kaempferol-4'-glucoside binds with the three β- cyclodextrins in the same 1:1 stoichiometry. The rank order of stability constants is SBE-β-CD > HP-β-CD > M-β-CD at the same pH level and pH 6.0 > pH 7.4 > pH 9.0 for the same cyclodextrin. The binding of kaempferol-4'-glucoside with the three β-cyclodextrin derivatives is synergistically driven by enthalpy and entropy at pH 6.0 and enthalpy-driven at pH 7.4 and 9.0. The possible inclusion mode was that in the cavity of β-CD is included the planar benzopyranic-4-one part of the kaempferol-4'-glucoside.

Control program of the neutron four-circle-diffractometer P110 at the ORPHEE reactor/CEN Saclay

International Nuclear Information System (INIS)

Guth, H.; Paulus, H.; Reimers, W.; Heger, G.

1984-05-01

The four-circle diffractometer P110 for elastic neutron scattering on single crystals was installed at the ORPHEE reactor/CEN Saclay in 1982. The control progam, presented here, is a new update of the former program versions used at the FR2 reactor. Important improvements concerning reliability and handling of the diffractometer are added. (orig./HP) [de

Air pollution studies in terms of PM2.5, PM2.5-10, PM10, lead and black carbon in urban areas of Antananarivo-Madagascar

International Nuclear Information System (INIS)

Rasoazanany, E. O.; Andriamahenina, N. N.; Ravoson, H. N.; Raoelina Andriambololona; Randriamanivo, L. V.; Ramaherison, H.; Ahmed, H.; Harinoely, M.

2011-01-01

Atmospheric aerosols or particulate matters are chemically complex and dynamic mixtures of solid and liquid particles. Sources of particulate matters include both natural and anthropogenic processes. The present work consists in determining the concentrations of existing elements in the aerosols collected in Andravoahangy and in Ambodin Isotry in Antananarivo city (Madagascar). The size distribution of these elements and their main sources are also studied.The Total Reflection X-Ray Fluorescence spectrometer is used for the qualitative and quantitative analyses. The results show that the concentrations of the airborne particulate matters PM 2.5-10 are higher than those of PM 2.5 .The identified elements in the aerosol samples are Ti, Cr, Mn, Fe, Ni, Cu, Zn, Br, Sr and Pb. The average concentrations of these elements are also higher in the coarse particles than in the fine particles. The calculation of the enrichment factors by Mason's model shows that Cr, Ni, Cu, Zn, Br and Pb are of anthropogenic origins. The average concentrations of lead (2.8 ng.m -3 , 31.3 ng.m -3 and 19.6 ng.m -3 respectively in aerosols collected in Andravoahangy in 2007 and in 2008 and in Ambodin Isotry in 2008) are largely lower than the average concentration of 1.8 μg.m -3 obtained in 2000 in the Antananarivo urban areas. The concentration of black carbon is higher in the fine particles. The Air Quality Index category is variable in the two sites.

The High Performance Shape Memory Effect (HP-SME in Ni Rich NiTi Wires: In Situ X-Ray Diffraction on Thermal Cycling

Directory of Open Access Journals (Sweden)

Coduri Mauro

2015-01-01

Full Text Available A novel approach for using Shape Memory Alloys (SMA was recently proposed and named highperformance shape memory effect (HP-SME. The HP-SME exploits the thermal cycling of stress-induced martensite for producing extremely high mechanical work with a very stable functional fatigue behaviour in Ni rich NiTi alloy. The latter was found to differ significantly from the functional fatigue behaviour observed for conventional SMA. This study was undertaken in order to elucidate the microstructural modifications at the basis of this particular feature. To this purpose, the functional fatigue was coupled to in situ Synchrotron Radiation X-Ray Diffraction, by recording patterns on wires thermally cycled by Joule effect under a constant applied stress (800 MPa. The accurate analysis the line profile XRD data suggests the accumulation of defects upon functional cycling, while the fibre texture was not observed to change. The functional fatigue exhibits a very similar behaviour as the line broadening of XRD peaks, thus suggesting the accumulation of dislocations as the origin of the mechanism of the permanent deformation.

Enamel Mineral Content Changes After Bleaching With High and Low Hydrogen Peroxide Concentrations: Colorimetric Spectrophotometry and Total Reflection X-ray Fluorescence Analyses.

Science.gov (United States)

Pinto, Avd; Bridi, E C; Amaral, Flb; França, Fmg; Turssi, C P; Pérez, C A; Martinez, E F; Flório, F M; Basting, R T

The purpose of this study was to evaluate the calcium (Ca) and phosphorous (P) content in enamel bleached with high and low concentrations of hydrogen peroxide (HP) using Total Reflection X-Ray Fluorescence (TXRF) and colorimetric spectrophotometry (SPEC). Forty-eight sound human third molars were used. Their roots were embedded in polystyrene resin and immersed for seven days in an artificial saliva solution. Then they were distributed into six groups to receive the bleaching treatments. The agents of high HP concentration (for in-office use) evaluated were Whiteness HP Maxx/FGM (35% HP), Whiteness HP Blue/FGM (35% HP, 2% calcium gluconate), Pola Office+/SDI (37.5% HP, 5% potassium nitrate), and Opalescence Boost/Ultradent (38% HP, 1.1% ion fluoride, 3% potassium nitrate); these agents were applied to enamel in three sessions. The agents of low HP concentration (for home use) evaluated were Pola Day/SDI (9.5% HP) and White Class 10%/FGM (10% HP, potassium nitrate, calcium, fluoride), and these agents were applied for 14 days. Enamel microbiopsies were evaluated by TXRF and SPEC analysis before the bleaching treatment (baseline), during the treatment, and 14 days after the end of the treatment. For TXRF, the Kruskal-Wallis test showed that Ca and P were not influenced by agent (p>0.05). For SPEC, Pola Office+, Opalescence Boost, Pola Day, and White Class 10% caused a decrease of Ca over time; there was a significant decrease of P over time to Pola Office+ and White Class 10%. The Spearman test showed no correlation between the Ca (p=0.987; r 2 =-0.020) and P (p=0.728, r 2 =0.038) obtained by SPEC and TXRF. For TXRF and SPEC, changes in Ca and P during bleaching occurred independently of the HP concentration used.

Pyrid-2-yl and 2-CyanoPhenyl fused heterocyclic compounds as human P2X3 inhibitors: a combined approach based on homology modelling, docking and QSAR analysis.

Science.gov (United States)

Janardhan, Sridhara; Seth, Subhendu; Viswanadhan, Vellarkad N

2014-02-01

P2X receptors are hetero-oligomeric proteins that function as membrane ion channels and are gated by extracellular ATP. The hP2X[Formula: see text] subunit is a constituent of the channels on a subset of sensory neurons involved in pain signaling, where ATP released by damaged and inflamed tissue can initiate action potentials. Hence, the inhibition of ATP-activated P2X3 receptor is an exciting approach for the treatment of inflammatory and neuropathic pain. Recently, the crystal structures of zebrafish P2X4 (zP2X4) were obtained in closed, apo state (PDB ID: 3I5D) and ATP-bound, open state (PDB ID: 4DW1). These structures were used to develop a homology model of human P2X3 (hP2X3 in order to identify through docking studies, the binding modes of known P2X3 inhibitors and their key active site interactions, along with a pharmacophore-based 3D-QSAR model for a series of 136 Pyrid-2-yl and 2-CyanoPhenyl fused heterocyclic compounds. These 3D-QSAR models have been developed with different combinations of training and test set divisions obtained by random separation, Jarvis-Patrick clustering, K-means clustering and sphere exclusion methods. The best predictive 3D-QSAR model resulted in training set R2 of 0.75, internal test set Q2 of 0.74, Pearson-R value of 0.87 and root mean square error of 0.37. The information generated by the pharmacophore model and docking analyses using the homology model provides valuable clues to design novel potent hP2X3 inhibitors.

Liquid-Hydrogen-Cooled 450-hp Electric Motor Test Stand Being Developed

Science.gov (United States)

Kascak, Albert F.; Trudell, Jeffrey J.; Brown, Gerald V.

2005-01-01

With growing concerns about global warming, there is a need to develop pollution-free aircraft. One approach is to use hydrogen-fueled aircraft that use fuel cells or turbogenerators to produce electric power to drive the electric motors that turn the aircraft s propulsive fans. Hydrogen fuel would be carried as a liquid, stored at its boiling point of 20.5 K (-422.5 F). Conventional electric motors, however, are too heavy for aircraft propulsion. We need to develop high-power, lightweight electric motors (highpower- density motors). One approach is to increase the conductivity of the wires by cooling them with liquid hydrogen (LH2). This would allow superconducting rotors with an ironless core. In addition, the motor could use very pure aluminum or copper, substances that have low resistances at cryogenic temperatures. A preliminary design of a 450-hp LH2-cooled electric motor was completed and is being manufactured by a contractor. This motor will be tested at the NASA Glenn Research Center and will be used to test different superconducting materials such as magnesium diboride (MgB2). The motor will be able to operate at speeds of up to 6000 rpm.

Study of the reaction {sup 22}O(p,p') with MUST detector. Development of the Cs(Tl) part of MUST-2 detector; Etude de la reaction {sup 22}O(p,p') avec le detecteur MUST. Developpement de l'etage CsI(Tl) de l'ensemble MUST 2

Energy Technology Data Exchange (ETDEWEB)

Becheva, E

2004-11-01

Elastic and inelastic proton scattering on the unstable nuclei {sup 22}O was measured in inverse kinematics at the GANIL facility. A secondary beam of {sup 22}O at 46.6 MeV/A with intensity of {approx} 1000 pps, impinged on a (CH{sub 2}){sub n} target. Recoiling protons were detected in the silicon strip array MUST. We measured the angular distributions of the ground and 2{sub 1}{sup +} states of {sup 22}O. Phenomenological and microscopic analysis of the data were performed. The phenomenological analysis using a global potential parameterization of Becchetti and Greenlees and CH89 yields a value of the deformation parameter {beta}{sub p,p}, = 0.23{+-}0.04 for {sup 22}O, much lower than that of {sup 20}O. The ratio of neutron and proton matrix element M{sub n}/M{sub p} is found equal to 1.46{+-}0.50. The microscopic analysis used of densities and transition densities calculated within HFB and QRPA models respectively. Optical potential were obtained through both folding and JLM procedures. A ratio M{sub n}/M{sub p}=2.5{+-}1.0 is deduced. Contrary of {sup 20}O, {sup 22}O behaviours like a doubly magic nucleus, suggesting a pronounced sub-shell closure at N=14. To develop the study of direct reactions induced by radioactive beams, we have developed and built, a new multi-detector MUST II devoted to light charged particle detection. In this work we established the requirements for the CsI(Tl) detector stage, and test four CsI detector prototypes, constructed by the SCIONIX company. (author)

[A systematic review of biosynthesis of poly (3-hydroxypropionate)].

Science.gov (United States)

Chang, Le; Zhan, Yuanlong; Liu, Changli

2018-04-25

Poly (3-hydroxypropionate) (P3HP), a new member of thermoplastic of family polyhydroxyalkanoates (PHAs), has excellent characteristics of biodegradability and biocompatibility. By now no reports can be found about wild-type bacteria that naturally synthesize P3HP, so the main way to produce P3HP is chemical and biological methods. Chemical method by adding high cost 3-HP monomers or their structural analogs as precursors, has the drawbacks of toxicity, low effectiveness and high cost. Biological method using engineered strain may utilize inexpensive and renewable carbon source to produce P3HP and has gradually become more and more popular. We systematically review here the biosynthesis of P3HP research progress. The advantages and disadvantages of biosynthesis pathways of glycerol pathway, malonyl-CoA pathway and β-alanine pathway were analyzed.

Kanfenggou UHP Metamorphic Fragment in Eastern Qinling Orogen and Its Relationship to Dabie-Sulu UHP and HP Metamorphic Belts, Central China

Institute of Scientific and Technical Information of China (English)

Suo Shutian; Zhong Zengqiu; Zhou Hanwen; You Zhendong

2003-01-01

In the Central Orogenic Belt, China, two UHP metamorphic belts are discriminated mainly based on a detailed structural analysis of the Kanfenggou UHP metamorphic fragment exposed in the eastern Qinling orogen, and together with previous regional structural, petrological and geochronological data at the scale of the orogenic domain. The first one corresponds to the South Altun-North QaidamNorth Qinling UHP metamorphic belt. The other is the Dabie-Sulu UHP and HP metamorphic belts. The two UHP metamorphic belts are separated by a series of tectonic slices composed by the Qinling rock group, Danfeng rock group and Liuling or Foziling rock group etc. respectively, and are different in age of the peak UHP metamorphism and geodynamic implications for continental deep subduction and collision. Regional field and petrological relationships suggest that the Kanfenggou UHP metamorphic fragment that contains a large volume of the coesite- and microdiamond-bearing eclogite lenses is compatible with the structures recognized in the South Altun and North Qaidam UHP metamorphic fragments exposed in the western part of China, thereby forming a large UHP metamorphic belt up to 1 000 km long along the orogen strike. This UHP metamorphic belt represents an intercontinental deep subduction and collision belt between the Yangtze and Sino-Korean cratons, occurred during the Paleozoic. On the other hand, the well-constrained Dabie-Sulu UHP and HP metamorphic belts occurred mainly during Triassic time (250-220 Ma), and were produced by the intrucontinental deep subduction and collision within the Yangtze craton. The Kanfenggou UHP metamorphic fragment does not appear to link with the Dabie-Sulu UHP and HP metamorphic belts along the orogen. There is no reason to assume the two UHP metamorphic belts us a single giant deep subduction and collision zone in the Central Orogenic Belt situated between the Yangtze and Sino-Korean cratons. Therefore, any dynamic model for the orogen must account

Pain Catastrophizing and Anxiety are Associated With Heat Pain Perception in a Community Sample of Adults With Chronic Pain.

Science.gov (United States)

Terry, Marisa J; Moeschler, Susan M; Hoelzer, Bryan C; Hooten, W Michael

2016-10-01

The principle aim of this study was to investigate the associations between heat pain (HP) perception, pain catastrophizing, and pain-related anxiety in a heterogenous cohort of community-dwelling adults with chronic pain admitted to a 3-week outpatient pain rehabilitation program. All adults consecutively admitted to an outpatient pain rehabilitation program from July 2009 through January 2011 were eligible for study recruitment (n=574). Upon admission, patients completed the Pain Catastrophizing Scale (PCS), the short version of the Pain Anxiety Symptoms Scale (PASS-20), and HP perception was assessed using a standardized quantitative sensory testing (QST) method of levels. Greater PCS scores were significantly correlated with lower standardized values of HP threshold (HP 0.5) (P=0.006) and tolerance (HP 5) (P=0.003). In a multiple variable model adjusted for demographic and clinical factors known to influence HP perception, every 10-point increase in the PCS was associated with a -0.124 point change in HP 0.5 (P=0.014) and a -0.142 change in HP 5 (P=0.014) indicating that participants with higher PCS scores had lower HP thresholds and tolerances, respectively. Similarly, greater PASS-20 scores significantly correlated with lower standardized values of HP 0.5 and HP 5. In a multiple variable model, every 10-point increase in the PASS-20 was associated with a -0.084 point change in HP 0.5 (P=0.005) and a -0.116 point change in HP 5 (P=0.001) indicating that participants with higher PASS-20 scores had lower HP thresholds and tolerances, respectively. The findings of this study extend the use of a standardized method for assessing HP in a heterogenous sample of adults with chronic pain. Although pain catastrophizing shares significant variance with pain-related anxiety, our findings suggest that either measure would be appropriate for use in future studies that incorporate the QST method of levels.

Measurement of the tensor analyzing powers in the dd→3Hen and dd→3Hp reactions at RIKEN

International Nuclear Information System (INIS)

Ladygin, V.P.; Ladygina, N.B; Sakai, H.; Uesaka, T.

2000-01-01

A new experiment is proposed to measure the angular distribution of the tensor analyzing powers A yy , A xx , and A xz in the dd→ 3 Hen and dd→ 3 Hp reactions using polarized deuteron beam at RIKEN. These polarization observables are sensitive to the spin-momentum distribution of neutron (proton) in 3 He( 3 H) at short distances in the framework of one-nucleon exchange approximation. These measurements will provide new insight into the 3-nucleon system spin structure at distances unreachable at the moment using electromagnetic probes

Experiment list: SRX275500 [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and elimina...ted in 1972, (PMID: 6081590) 39717260,92.4,31.6,2510 GSM1133482: U2OS DMSO p53chip;

Determination of the approximate number of welding qualification procedures, according to the AD-HP 2/1 and NUCLEN rules

International Nuclear Information System (INIS)

Couto, J.G.V.

1986-01-01

The simple equations based on the phylosophy of AD-HP 2/1 which determine the approximate number welding qualification procedures for the manufacture either of one component, or several components requested simultaneously are presented. It is also intended to help the manufacturer in the rational preparation of scheduling the required minimum qualifications for performing the work, including giving him an idea about the involved costs which the firm will have to bear in this phase prior to manufacturing. (Author) [pt

Early Stage Blood Purification for Paraquat Poisoning: A Multicenter Retrospective Study.

Science.gov (United States)

Li, An; Li, Wenxiong; Hao, Fengtong; Wang, Haishi

2016-01-01

To evaluate the efficacy of conservative treatment vs. hemoperfusion (HP) vs. HP + continuous veno-venous hemofiltration (CVVH) for acute Paraquat (PQ) poisoning. This was a multicenter retrospective study of patients with PQ poisoning between January 2013 and June 2014. Clinical data and PQ serum levels were collected at baseline and after 24, 48, and 72 h of treatment. Seventy-five, 65, and 43 underwent conservative treatment only (conservative treatment group), conservative treatment + HP (HP group), and conservative treatment + HP + CVVH (HP + CVVH group), respectively. PQ serum levels decreased in all groups after 72 h of treatment (p treatment group. More importantly, PQ blood levels were significantly lower in the HP + CVVH group compared with the HP group at 24 h (p treatment group (p poisoning could decrease PQ blood levels, alleviate organ damage, and increase survival. © 2016 S. Karger AG, Basel.

HP-CsB{sub 5}O{sub 8}. Synthesis and characterization of an outstanding borate exhibiting the simultaneous linkage of all structural units of borates

Energy Technology Data Exchange (ETDEWEB)

Sohr, Gerhard; Huppertz, Hubert [Institut fuer Allgemeine, Anorganische und Theoretische Chemie, Leopold-Franzens-Universitaet Innsbruck (Austria); Toebbens, Daniel M. [Helmholtz-Zentrum Berlin fuer Materialien und Energie GmbH, Berlin (Germany); Schmedt auf der Guenne, Joern [Department fuer Chemie/Biologie, Universitaet Siegen (Germany)

2014-12-15

The new cesium pentaborate HP-CsB{sub 5}O{sub 8} is synthesized under high-pressure/high-temperature conditions of 6 GPa and 900 C in a Walker-type multianvil apparatus. The compound crystallizes in the orthorhombic space group Pnma (Z=4) with the parameters a=789.7(1), b=961.2(1), c=836.3(1) pm, V=0.6348(1) nm{sup 3}, R{sub 1}=0.0359 and wR{sub 2}=0.0440 (all data). The new structure type of HP-CsB{sub 5}O{sub 8} exhibits the simultaneous linkage of trigonal BO{sub 3} groups, corner-sharing BO{sub 4} tetrahedra, and edge-sharing BO{sub 4} tetrahedra including the presence of threefold-coordinated oxygen atoms. With respect to the rich structural chemistry of borates, HP-CsB{sub 5}O{sub 8} is the second structure type possessing this outstanding combination of the main structural units of borates in one compound. The structure consists of corrugated chains of corner- and edge-sharing BO{sub 4} tetrahedra interconnected through BO{sub 3} groups forming octagonal channels. Inside these channels, cesium is 13+3-fold coordinated by oxygen atoms. {sup 11}B MQMAS NMR spectra are analyzed to estimate the isotropic chemical shift values and quadrupolar parameters. IR and Raman spectra are obtained and compared to the calculated vibrational frequencies at the Γ-point. The high-temperature behavior is examined by means of temperature-programmed powder diffraction. (copyright 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

Effect of Hydrogen and Carbamide Peroxide in Bleaching, Enamel Morphology, and Mineral Composition: In vitro Study.

Science.gov (United States)

Llena, Carmen; Esteve, Irene; Forner, Leopoldo

2017-07-01

The aim of the study was to evaluate the bleaching effect, morphological changes, and variations in calcium (Ca) and phosphate (P) in the enamel with hydrogen peroxide (HP) and carbamide peroxide (CP) after the use of different application regimens. Four groups of five teeth were randomly assigned, according to the treatment protocol: HP 37.5% applied for 30 or 60 minutes (HP30, HP60), CP 16% applied for 14 or 28 hours (CP14, CP28). Changes in dental color were evaluated, according to the following formula: ΔE = [(L a -L b ) 2 +(a a -a b ) 2 + (b a -b b ) 2 ] 1/2 . Enamel morphology and Ca and P compositions were evaluated by confocal laser scanning microscope and environmental scanning electron microscopy. ΔE HP30 was significantly greater than CP14 (10.37 ± 2.65/8.56 ± 1.40), but not between HP60 and CP28. HP60 shows greater morphological changes than HP30. No morphological changes were observed in the groups treated with CP. The reduction in Ca and P was significantly greater in HP60 than in CP28 (p tooth color; HP produced morphological changes and Ca and P a gradual decrease, while CP produced no morphological changes, and the decrease in mineral component was smaller. CP 16% applied during 2 weeks could be equally effective and safer for tooth whitening than to administer two treatment sessions with HP 37.5%.

Dosimeter configurations for the measurement of H_s(0.07) and H_p(10) from photons

DEFF Research Database (Denmark)

Christensen, P.; Bøtter-Jensen, L.; Ennow, K.

1990-01-01

F TLD-700, LiF:Mg, Cu, P, and Li2B4O7:Mn TL detectors were investigated for the measurement of H(p) (10), and graphite-mixed MgB4O7:Dy, LiF and Li2B4O7 were investigated for the measurement of H(s) (0.07). Irradiations were made with a Co-60 gamma source and ISO qualities of X radiations...

Comparison of Spot Urine Protein to Creatinine Ratio to 24-Hour Proteinuria to Identify Important Change Over Time in Proteinuria in Lupus.

Science.gov (United States)

Medina-Rosas, Jorge; Su, Jiandong; Cook, Richard J; Sabapathy, Arthy; Touma, Zahi

2017-09-01

The aim of this study was to determine whether spot urine protein-to-creatinine ratio (PCR) accurately measures the change in proteinuria compared with 24-hour proteinuria (24H-P). This was a retrospective analysis on patients' paired visits and paired urine samples for PCR and 24H-P. Patients with both abnormal 24H-P (>0.5 g/d) and PCR (>0.05 g/mmol) or both normal 24H-P (≤0.5 g/d) and PCR (≤0.05 g/mmol) at baseline visit were identified.The first follow-up visit with partial recovery (50% decrease in proteinuria) or complete recovery (≤0.5 g/d) was identified for those with abnormal baseline 24H-P, and new proteinuria (>0.5 g/d) was identified for those with normal 24H-P. Twenty-four-hour urine collection and PCR end-point frequencies were compared. Twenty-four-hour urine collection results were converted to 24H-PCR. Twenty-four-hour PCR and PCR were utilized to measure the magnitude of change (by standardized response mean [SRM]) in patients who achieved the end points. Of 230 patients, at baseline, 95 patients had abnormal and 109 had normal 24H-P and PCR. On follow-up, 57 achieved partial recovery, and 53 achieved complete recovery by 24H-P. Standardized response mean was -1.03 and -1.10 for 24H-PCR and PCR, respectively. By PCR, 53 patients had partial recovery, and 27 had complete recovery. Standardized response mean was -1.25 and -0.86 by 24H-PCR and PCR, respectively.For new proteinuria, 28 patients were identified by 24H-P and 21 by PCR. Twenty-four-hour PCR SRM was 0.80, and PCR SRM was 0.68. Protein-to-creatinine ratio does not have sufficient accuracy compared with 24H-P for improvement and worsening to be used in lieu of 24H-P.

Plasma disruption forces: a program for the HP 9845 and 9872 B plotter

International Nuclear Information System (INIS)

Foss, M.H.

1982-11-01

A computer program is described for the calculation of currents, forces, torques, and voltages in the conducting first wall and limiter of a tokamak reactor experiencing a plasma quench. Instructions are given for running the program on a HP 9845 B desk-top computer with a 9872 B plotter. The first wall and limiter have been modelled by 44 coaxial loops. The inductance matrix among the loops is calculated, and the currents in them are calculated step-by-step over time, driven by the flux from the decaying plasma current and subject to the field from the plasma, from the poloidal field coils, and from all current loops. An insulating break in the limiter or first wall can be modelled by imposing a back voltage which causes all the currents in the limiter or first wall to sum to zero. This potential is related to the voltage between segments of a segmented limiter or first wall

The effect of high pressure and residual oxygen on the color stability of minced cured restructured ham at different levels of drying, pH, and NaCl.

Science.gov (United States)

Bak, Kathrine Holmgaard; Lindahl, Gunilla; Karlsson, Anders H; Lloret, Elsa; Gou, Pere; Arnau, Jacint; Orlien, Vibeke

2013-10-01

Color stability of minced cured restructured ham was studied by considering the effects of high pressure (HP) (600 MPa, 13°C, 5 min), raw meat pH24 (low, normal, high), salt content (15, 30 g/kg), drying (20%, 50% weight loss), and residual oxygen level (0.02%-0.30%). Raw hams were selected by pH24 in Semimembranosus, mixed with additives, frozen, sliced, and dried by the Quick-Dry-Slice® (QDS) process followed by HP treatment or not (control). Packaging and storage simulated industrial packaging: modified atmosphere containing 80% N2, 20% CO2, and residual O2 in one of three intervals: hams, while the stabilizing effect on 50% QDS hams was smaller, concluding that water has the dominating role. Raw meat pH24, salt content, and residual oxygen level had varying effects on the stability of the red color. Copyright © 2013 Elsevier Ltd. All rights reserved.

A portable marine geophysical data access and management system

Digital Repository Service at National Institute of Oceanography (India)

Kunte, P.D.; Narvekar, P.

Geophysical Oracle Database Management System (GPODMS) that is residing on UNIX True 64 Compaq Alpha server. GPODMS is a stable Oracle database system for longterm storage and systematic management of geophysical data and information of various disciplines...

Eclogites and garnet clinopyroxenites in the Anrakhai complex, Central Asian Orogenic Belt, Southern Kazakhstan: P-T evolution, protoliths and some geodynamic implications

Science.gov (United States)

Pilitsyna, Anfisa V.; Tretyakov, Andrey A.; Degtyarev, Kirill E.; Cuthbert, Simon J.; Batanova, Valentina G.; Kovalchuk, Elena V.

2018-03-01

The Anrakhai Metamorphic Complex (AMC), located in the SE part of the Chu-Ili Mountains of Southern Kazakhstan in the western part of Central Asian Orogenic Belt, exhibits occurrences of HP metamorphic rocks in the form of eclogites and garnet clinopyroxenites with peak metamorphic conditions of 750-850° and 15-19 kbar estimated with both conventional geothermobarometric methods and phase diagram modeling. P-T estimates as well as intimate field relations evidently imply a common metamorphic history for eclogites and garnet clinopyroxenites of the AMC. These high-pressure, medium temperature eclogite facies P-T conditions are indicative of a collision or subduction tectonic setting. Major and trace element geochemistry suggests that they probably had a common magmatic origin as part of a suite of differentiated tholeiitic intrusions. Furthermore, distinctive mineral and chemical compositions of these eclogites and garnet clinopyroxenites correspond to the Fe-Ti type of ultramafic rocks suggesting that they may have been derivatives of intraplate tholeiitic melts, introduced into continental crust before HP metamorphism.

pH-dependent structural change of the extracellular sensor domain of the DraK histidine kinase from Streptomyces coelicolor.

Science.gov (United States)

Yeo, Kwon Joo; Kim, Eun Hye; Hwang, Eunha; Han, Young-Hyun; Eo, Yumi; Kim, Hyun Jung; Kwon, Ohsuk; Hong, Young-Soo; Cheong, Chaejoon; Cheong, Hae-Kap

2013-02-15

Recently, the DraR/DraK (Sco3063/Sco3062) two-component system (TCS) of Streptomycescoelicolor has been reported to be involved in the differential regulation of antibiotic biosynthesis. However, it has not been shown that under which conditions and how the DraR/DraK TCS is activated to initiate the signal transduction process. Therefore, to understand the sensing mechanism, structural study of the sensory domain of DraK is highly required. Here, we report the biochemical and biophysical properties of the extracellular sensory domain (ESD) of DraK. We observed a reversible pH-dependent conformational change of the ESD in a pH range of 2.5-10. Size-exclusion chromatography and AUC (analytical ultracentrifugation) data indicated that the ESD is predominantly monomeric in solution and exists in equilibrium between monomer and dimer states in acidic condition. Using NMR (nuclear magnetic resonance) and CD (circular dichroism) spectroscopy, our findings suggest that the structure of the ESD at low pH is more structured than that at high pH. In particular, the glutamate at position 83 is an important residue for the pH-dependent conformational change. These results suggest that this pH-dependent conformational change of ESD may be involved in signal transduction process of DraR/DraK TCS. Copyright © 2013 Elsevier Inc. All rights reserved.

Evaluation of several clinical parameters after bleaching with hydrogen peroxide at different concentrations: A randomized clinical trial.

Science.gov (United States)

Lima, Suellen Nogueira Linares; Ribeiro, Izabella Santos; Grisotto, Marcos Augusto; Fernandes, Elizabeth Soares; Hass, Viviane; de Jesus Tavarez, Rudys Rodolfo; Pinto, Shelon Cristina Souza; Lima, Darlon Martins; Loguercio, Alessandro D; Bandeca, Matheus Coelho

2018-01-01

This randomized double-blind clinical trial compared tooth sensitivity (TS), bleaching efficacy, and cytokine levels after applying in-office bleaching treatments containing 15% and 35% hydrogen peroxide (HP15% and HP35%, respectively). Twenty-five volunteers were randomly assigned to receive HP15% or HP35% treatment. The bleaching agent was applied in three 15-min applications per session. Two bleaching sessions were separated by a 1-week interval. The participants scored TS using a visual analog scale and numerical rating scale. Bleaching efficacy was determined by subjective and objective methods. Gingival crevicular fluid was collected from three jaws sites per patient for the analysis of fluid volume. Flow cytometry was used to analyze gingival crevicular fluid levels of interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-10, tumor necrosis factor, and interferon-gamma. All measurements were obtained before and after bleaching. All data were statistically analyzed (α=0.05). The absolute risk and intensity of TS was higher for HP35% than for HP15% (p>0.002). One month post-bleaching, HP35% produced more bleaching than HP15% (p=0.02). However patient perception (p=0.06) and patient satisfaction (p=0.53) with regard to bleaching were not significantly different. No significant differences existed in the gingival fluid volume (p>0.38) or in any cytokine level (p>0.05) for either HP concentration. Treatment: with HP35% is more effective than HP15%, but generates a greater risk and intensity of TS. No inflammatory changes occurred despite the difference in the HP concentrations. Hydrogen peroxide at a lower concentration (e.g., 15%) should be considered a good treatment alternative for in-office bleaching because the higher concentration for in-office bleaching generates a greater risk and intensity of TS for patients. Copyright © 2017 Elsevier Ltd. All rights reserved.

Growth promotion in pigs by oxytetracycline coincides with down regulation of serum inflammatory parameters and of hibernation-associated protein HP-27.

Science.gov (United States)

Soler, Laura; Miller, Ingrid; Hummel, Karin; Razzazi-Fazeli, Ebrahim; Jessen, Flemming; Escribano, Damian; Niewold, Theo

2016-05-01

The growth promoting effect of supplementing animal feed with antibiotics like tetracycline has traditionally been attributed to their antibiotic character. However, more evidence has been accumulated on their direct anti-inflammatory effect during the last two decades. Here we used a pig model to explore the systemic molecular effect of feed supplementation with sub therapeutic levels of oxytetracycline (OTC) by analysis of serum proteome changes. Results showed that OTC promoted growth, coinciding with a significant down regulation of different serum proteins related to inflammation, oxidation and lipid metabolism, confirming the anti-inflammatory mechanism of OTC. Interestingly, apart from the classic acute phase reactants also down regulation was seen of a hibernation associated plasma protein (HP-27), which is to our knowledge the first description in pigs. Although the exact function in non-hibernators is unclear, down regulation of HP-27 could be consistent with increased appetite, which is possibly linked to the anti-inflammatory action of OTC. Given that pigs are good models for human medicine due to their genetic and physiologic resemblance, the present results might also be used for rational intervention in human diseases in which inflammation plays an important role such as obesity, type 2 diabetes and cardiovascular diseases. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Test report for run-in acceptance testing of Project W-151 300 HP mixing pumps

International Nuclear Information System (INIS)

Berglin, B.G.

1998-01-01

This report documents the results of a performance demonstration and operational checkout of three 300 HP mixer pumps in accordance with WHC-SD-WI51-TS-001 ''Mixer Pump Test Specification for Project W-151'' and Statement of Work 8K520-EMN-95-004 ''Mixer Pump Performance Demonstration at MASF'' in the 400 Area Maintenance and Storage Facility (MASF) building. Testing of the pumps was performed by Fast Flux Test Facility (FFTF) Engineering and funded by the Tank Waste Remediation System (TWRS) Project W-151. Testing began with the first pump on 04-01-95 and ended with the third pump on 11-01-96. Prior to testing, the MASF was modified and prepared to meet the pump testing requirements set forth by the Test Specification and the Statement of Work

[Pr2(pdc3(Hpdc(H2O4]n·n(H3hp·8n(H2O, a One-Dimensional Coordination Polymer Containing PrO6N3 Tri-Capped Trigonal Prisms and PrO8N Mono-Capped Square Anti-Prisms (H2pdc = Pyridine 2,6-Dicarboxylic Acid, C7H5NO4; 3hp = 3-Hydroxy Pyridine, C5H5NO

Directory of Open Access Journals (Sweden)

Shahzad Sharif

2012-08-01

Full Text Available The synthesis, structure and some properties of the one-dimensional coordination polymer, [Pr2(pdc3(Hpdc]n·n(H3hp·8n(H2O, (H2pdc = pyridine 2,6-dicarboxylic acid, C7H5NO4; 3hp = 3-hydroxypyridine, C5H5NO are described. One of the Pr3+ ions is coordinated by two O,N,O-tridentate pdc2− ligands and one tridentate Hpdc− anion to generate a fairly regular PrO6N3 tri-capped trigonal prism, with the N atoms acting as the caps. The second Pr3+ ion is coordinated by one tridentate pdc2− dianion, four water molecules and two monodentate bridging pdc2− ligands to result in a PrO8N coordination polyhedron that approximates to a mono-capped square-anti-prism. The ligands bridge the metal-atom nodes into a chain, which extends in the [100] direction. The H3hp+ cation and uncoordinated water molecules occupy the inter-chain regions and an N–HLO and numerous O–HLO hydrogen bonds consolidate the structure. The H3hp+ species appears to intercalate between pendant pdc rings to consolidate the polymeric structure. Crystal data: 1 (C33H43N5O29Pr2, Mr = 1255.54, triclinic,  (No. 2, Z = 2, a = 13.2567(1 Å, b = 13.6304(2 Å, c = 13.6409(2 Å, α = 89.695(1°, β = 63.049(1°, γ = 86.105(1°, V = 2191.16(5 Å3, R(F = 0.033, wR(F2 = 0.084.

Haptoglobin concentrations in free-range and temporarily captive juvenile steller sea lions.

Science.gov (United States)

Thomton, Jamie D; Mellish, Jo-Ann E

2007-04-01

Haptoglobin (Hp) is an acute-phase protein synthesized in the liver that circulates at elevated concentrations in response to tissue damage caused by inflammation, infection, and trauma. As part of a larger study, sera Hp concentrations were measured in temporarily captive (n = 21) and free-range (n = 38) western stock juvenile Steller sea lions (Eumetopias jubatus) sampled from 2003 to 2006. Baseline Hp concentration at time of capture was 133.3 +/- 17.4 mg/dl. Temporarily captive animals exhibited a 3.2-fold increase in Hp concentrations during the first 4 wk of captivity, followed by a return to entry levels by week 5. Haptoglobin levels were not influenced by age, season, or parasite load. There was a significant positive correlation between Hp concentrations and white blood cell count (P < 0.001) and globulin levels (P < 0.001) and a negative correlation to red blood cell count and hematocrit (P < 0.001 for both). There was no correlation between Hp levels and platelet count (P = 0.095) or hemoglobin (P = 0.457). Routine blubber biopsies collected under gas anesthesia did not produce a measurable Hp response. One animal with a large abscess had an Hp spike of 1,006.0 mg/dl that returned to entry levels after treatment. In conclusion, serum Hp levels correlate to the stable clinical health status observed during captivity, with moderate Hp response during capture and initial acclimation to captivity and acute response to inflammation and infection.

Culture promotes transfer of thyroid epithelial cell hyperplasia and proliferation by reducing regulatory T cell numbers.

Science.gov (United States)

Kayes, Timothy D; Braley-Mullen, Helen

2013-01-01

IFN-γ(-/-) NOD.H-2h4 mice develop a spontaneous autoimmune thyroid disease, thyroid epithelial cell hyperplasia and proliferation (TEC H/P) when given NaI in their water for 7+ mo. TEC H/P can be transferred to IFN-γ(-/-) SCID mice by splenocytes from mice with severe (4-5+) disease, and transfer of TEC H/P is improved when splenocytes are cultured prior to transfer. Older (9+ mo) IFN-γ(-/-) NOD.H-2h4 mice have elevated numbers of FoxP3(+) T reg cells, up to 2-fold greater than younger (2 mo) mice. During culture, the number of T reg decreases and this allows the improved transfer of TEC H/P. Co-culture with IL-2 prior to transfer prevents the decrease of T reg and improves their in vitro suppressive ability resulting in reduced TEC H/P in recipient mice. Therefore, culturing splenocytes improves transfer of TEC H/P by reducing the number of T reg and IL-2 inhibits transfer by preserving T reg number and function. Copyright © 2013 Elsevier Inc. All rights reserved.

Differential cross section measurements for $\gamma n\to {\pi }^{-}\mathrm{<span\; class="hlt">p</span>}$ above the first nucleon resonance region

Energy Technology Data Exchange (ETDEWEB)

Mattione, P. T.; Carman, D. S.; Strakovsky, I. I.; Workman, R. L.; Kudryavtsev, A. E.; Svarc, A.; Tarasov, V. E.; Adhikari, K. P.; Adhikari, S.; Adikaram, D.; Akbar, Z.; Anefalos Pereira, S.; Ball, J.; Baltzell, N. A.; Bashkanov, M.; Battaglieri, M.; Batourine, V.; Bedlinskiy, I.; Biselli, A. S.; Boiarinov, S.; Briscoe, W. J.; Burkert, V. D.; Cao, T.; Celentano, A.; Charles, G.; Chetry, T.; Ciullo, G.; Clark, L.; Cole, P. L.; Contalbrigo, M.; Cortes, O.; Crede, V.; D' Angelo, A.; Dashyan, N.; De Vita, R.; De Sanctis, E.; Defurne, M.; Deur, A.; Djalali, C.; Dugger, M.; Dupre, R.; Egiyan, H.; El Alaoui, A.; El Fassi, L.; Eugenio, P.; Fedotov, G.; Fersch, R.; Filippi, A.; Fleming, J. A.; Fradi, A.; Ghandilyan, Y.; Gilfoyle, G. P.; Giovanetti, K. L.; Girod, F. X.; Gleason, C.; Golovatch, E.; Gothe, R. W.; Griffioen, K. A.; Guidal, M.; Hafidi, K.; Hakobyan, H.; Hanretty, C.; Harrison, N.; Hattawy, M.; Heddle, D.; Hicks, K.; Hollis, G.; Holtrop, M.; Hughes, S. M.; Ilieva, Y.; Ireland, D. G.; Ishkhanov, B. S.; Isupov, E. L.; Jenkins, D.; Jiang, H.; Jo, H. S.; Joo, K.; Joosten, S.; Keller, D.; Khachatryan, G.; Khachatryan, M.; Khandaker, M.; Kim, A.; Kim, W.; Klein, A.; Klein, F. J.; Kubarovsky, V.; Kuleshov, S. V.; Lanza, L.; Lenisa, P.; Livingston, K.; MacGregor, I. J. D.; Markov, N.; McKinnon, B.; Meyer, C. A.; Meziani, Z. E.; Mineeva, T.; Mokeev, V.; Montgomery, R. A.; Movsisyan, A.; Munoz Camacho, C.; Murdoch, G.; Nadel-Turonski, P.; Net, L. A.; Niccolai, S.; Niculescu, G.; Niculescu, I.; Osipenko, M.; Ostrovidov, A. I.; Paolone, M.; Paremuzyan, R.; Park, K.; Pasyuk, E.; Phelps, W.; Pisano, S.; Pogorelko, O.; Price, J. W.; Procureur, S.; Prok, Y.; Protopopescu, D.; Raue, B. A.; Ripani, M.; Ritchie, B. G.; Rizzo, A.; Rosner, G.; Sabatié, F.; Salgado, C.; Schumacher, R. A.; Sharabian, Y. G.; Simonyan, A.; Skorodumina, Iu.; Smith, G. D.; Sokhan, D.; Sparveris, N.; Stankovic, I.; Stepanyan, S.; Strauch, S.; Taiuti, M.; Ungaro, M.; Voskanyan, H.; Voutier, E.; Walford, N. K.; Watts, D.; Wei, X.; Wood, M. H.; Zachariou, N.; Zhang, J.; Zhao, Z. W.

2017-09-01

The quasifree gamma d -> pi(-)p(p) differential cross section has been measured with CLAS at photon beam energies E-gamma from 0.445 to 2.510 GeV (corresponding to W from 1.311 to 2.366 GeV) for pion center-of-mass angles cos theta(c.m.)(pi) from -0.72 to 0.92. A correction for final state interactions has been applied to these data to extract the gamma n -> pi(-)p differential cross sections. These cross sections are quoted in 8428 (E-gamma, cos theta(c.m)(pi)) bins, a factor of nearly 3 increase in the world statistics for this channel in this kinematic range. These new data help to constrain coupled-channel analysis fits used to disentangle the spectrum of N* resonances and extract their properties. Selected photon decay amplitudes N* -> gamma n at the resonance poles are determined for the first time and are reported here.

Finite element analysis of the Girkmann problem using the modern hp-version and the classical h-version

KAUST Repository

Niemi, Antti

2011-06-03

We perform finite element analysis of the so called Girkmann problem in structural mechanics. The problem involves an axially symmetric spherical shell stiffened with a foot ring and is approached (1) by using the axisymmetric formulation of linear elasticity theory and (2) by using a dimensionally reduced shell-ring model. In the first approach the problem is solved with a fully automatic hp-adaptive finite element solver whereas the classical h-version of the finite element method is used in the second approach. We study the convergence behaviour of the different numerical models and show that accurate stress resultants can be obtained with both models by using effective post-processing formulas. © Springer-Verlag London Limited 2011.

High-pressure granulites in the Fuping Complex of the central North China Craton: Metamorphic P-T-t evolution and tectonic implications

Science.gov (United States)

Qian, Jiahui; Yin, Changqing; Zhang, Jian; Ma, Li; Wang, Luojuan

2018-04-01

Mafic granulites in the Fuping Complex occur as lenses or boudins within high-grade TTG (Trondhjemite-Tonalite-Granodiorite) gneisses. Petrographic observations reveal four generations of mineral assemblage in the granulites: an inclusion assemblage of hornblende + plagioclase + ilmenite + quartz within garnet core; an inferred peak assemblage composed of garnet ± hornblende + plagioclase + clinopyroxene + rutile/ilmenite + quartz; a decompression assemblage characterized by symplectites of clinopyroxene ± orthopyroxene + plagioclase, coronae of plagioclase ± clinopyroxene ± hornblende around embayed garnet porphyroblasts or a two-pyroxene association; and a late amphibolite-facies retrogressive assemblage. Two representative samples were used for pseudosection modeling in NCFMASHTO model system to determine their metamorphic evolution. The results show that these granulites experienced a high-pressure stage of metamorphism with peak P-T conditions of 12-13 kbar and 760-800 °C (Pmax) and a post-peak history under P-T conditions of ∼9.0 kbar and 805-835 °C (Tmax), indicating a nearly isothermal decompression process (ITD) with a slight heating. Metamorphic evolution from the Pmax to the Tmax is predicted to be dominated by garnet breakdown through continuous metamorphic reactions of garnet + quartz ± diopside = hornblende + plagioclase + liquid and garnet + quartz + hornblende = plagioclase + diopside + liquid + orthopyroxene. Further metamorphic evolution after the Tmax is dominated by cooling, suggesting that high-pressure (HP) granulites may also exist in the Fuping Complex. Metamorphic zircons in the Fuping HP mafic granulites have left inclined REE patterns, Ti contents of 1.68-6.88 ppm and crystallization temperatures of 602-712 °C. SIMS zircon U-Pb dating on these zircons yields 207Pb/206Pb ages of 1891 ± 14 Ma and 1849 ± 6 Ma, interpreted to represent the cooling stage of metamorphism. The P-T-t evolution of the Fuping HP mafic granulites records

Influence of the geometrical characteristics of an HpGe detector on its efficiency

International Nuclear Information System (INIS)

Vargas, M.J.; Timon, A.F.; Sanchez, D.P.

2002-01-01

Computer codes based on Monte Carlo calculations have been extensively developed for the computation of the efficiency in gamma-ray spectrometry. The errors in the specific parameters of the detector due to the lack of precise knowledge of its characteristics usually represent one of the most important sources of inaccuracy in this simulation technique. Influence of several detector parameters on the efficiency for a typical coaxial n-type HpGe detector is presented. Calculations of the full-energy peak efficiencies were performed by means of a Monte Carlo code in the range 122-1836 keV for several types of source configuration: point source, cellulose filter, and two different cylindrical boxes containing a solid matrix of SiO 2 . The detector parameters varied were the crystal diameter, crystal height, diameter of the internal core, and the position of the crystal with respect to the beryllium window. Significant deviations in the efficiency, depending on the source geometry and the photon energy, can be produced by varying only slightly some of the detector parameters. (author)

Variation in bull beef quality due to ultimate muscle pH is correlated to endopeptidase and small heat shock protein levels.

Science.gov (United States)

Pulford, D J; Dobbie, P; Fraga Vazquez, S; Fraser-Smith, E; Frost, D A; Morris, C A

2009-09-01

This study set out to determine if ultimate pH (pH(u)) affected the performance of intracellular small heat shock protein and endopeptidase dynamics in muscle during beef ageing. Longissimus dorsi muscles from 39 Angus or Limousin×Angus bulls were examined to see if pH(u) achieved at 22h post mortem (rigor) affected tenderness and water holding capacity of beef. Samples were segregated into three pH(u) groups termed high (pH>6.3), intermediate (5.7pHpHpH(u) beef. More than 30% of bull beef did not achieve acceptable tenderness at 8 days post mortem with this ageing regime. No significant differences in calpain or cathepsin enzyme levels due to meat pH were observed until after 22h post mortem, but low pH(u) beef had elevated caspase 3/7 activity soon after slaughter. At 22h post mortem, greater levels of μ-calpain enzyme were found in the high and intermediate pH(u) beef and cathepsin B levels were superior in the low pH(u) beef after 2 days post mortem. Different rates of desmin and troponin T protein degradation were also observed in aged bull beef. Both proteins were degraded within 6h post mortem for high pH(u) beef, but took >3 days post mortem for intermediate pH(u) beef. High levels of alpha β-crystallin (aβC) at 22h post mortem coincided with delayed muscle protein degradation for low pH(u) beef. Our results support the hypothesis that aβC shields myofibrils and buffers against endopeptidase degradation of beef structure during ageing.

Mesh wrapping for severe hepatic injury: a beneficial option in the trauma surgeon's armamentarium.

Science.gov (United States)

Kennedy, Ryan; Brevard, Sidney B; Bosarge, Patrick; Simmons, Jon D; Frotan, Mohammed A; Baker, Jeremy A; Tuart, Lynda; Pritchett, Cindy; Gonzalez, Richard P

2015-03-01

The purpose of this study was to assess the efficacy of absorbable mesh wrapping (MW) versus perihepatic packing (HP) for severe hepatic injury. From January 2001 to December 2012, data were collected for MW patients with hepatic injury. Patients who underwent HP were matched with MW patients by injury mechanism, liver injury grade, Injury Severity Score, and age. Twenty-six MW and twenty-six HP patients were matched. Eighteen blunt and 8 penetrating injuries were present in each group. There were 9 (35%) mortalities in the MW group and 16 (62%) in the HP groups (P = .03). Average transfusions were 12.0 and 24.5 (P = .03) packed red blood cells in the MW and HP groups, respectively. Average laparotomies per survivors were 1.3 for the MW and 3.1 for the HP groups (P = .01). Average length of stay for survivors was 19 and 47 (P = .04) days in the MW and HP groups, respectively. MW for hepatic injuries can significantly decrease mortality, transfusions, laparotomies, and length of stay. Copyright © 2015 Elsevier Inc. All rights reserved.

GPIB interface of IBM-PC compatible microcomputer and multimeter HP3457A for the automatization of temperature measurements

International Nuclear Information System (INIS)

Migliano, A.C.C.; Sircilli Neto, F.; Passaro, A.

1990-01-01

The measurement of temperature distribution of the beam transport devices, utilized in the IEAv linac, takes several hours to be completed. At the beam transport laboratory, these experiments are controlled with the help of a MS-quick BASIC code denominated TERMOJ. This code uses the software of STD-8410 plate to promote the communication between an IBM-PC compatible microcomputer and the programmable multimeter HP3457A via GPIB interface. This work presents information for the automation of experiments using thermocouples to obtain temperature distributions with the TERMOJ code. Also, suggestions of how modify the algorithm of the program for the specific situations are indicated. (author)

Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3-HP1-Me(3)K20H4 pathway.

Science.gov (United States)

Bongiorni, Silvia; Pasqualini, Barbara; Taranta, Monia; Singh, Prim B; Prantera, Giorgio

2007-03-15

Using RNA interference (RNAi) we have conducted a functional analysis of the HP1-like chromobox gene pchet2 during embryogenesis of the mealybug Planococcus citri. Knocking down pchet2 expression results in decondensation of the male-specific chromocenter that normally arises from the developmentally-regulated facultative heterochromatinisation of the paternal chromosome complement. Together with the disappearance of the chromocenter the staining levels of two associated histone modifications, tri-methylated lysine 9 of histone H3 [Me(3)K9H3] and tri-methylated lysine 20 of histone H4 [Me(3)K20H4], are reduced to undetectable levels. Embryos treated with double-stranded RNA (dsRNA) targeting pchet2 also exhibit chromosome abnormalities, such as aberrant chromosome condensation, and also the presence of metaphases that contain 'lagging' chromosomes. We conclude that PCHET2 regulates chromosome behavior during metaphase and is a crucial component of a Me(3)K9H3-HP1-Me(3)K20H4 pathway involved in the facultative heterochromatinisation of the (imprinted) paternal chromosome set.

Usefulness of high helical pitch acquisition for reduction of patient radiation dose in cardiac multidetector computed tomography

International Nuclear Information System (INIS)

Sano, Tomonari; Matsutani, Hideyuki; Kondo, Takeshi; Sekine, Takako; Arai, Takehiro; Morita, Hitomi; Takase, Shinichi

2009-01-01

Helical pitch (HP) usually has been decided automatically by the software (Heart Navi) included in the MDCT machine (Aquilion 64) depending on gantry rotation speed (r) and heart rate (HR). To reduce radiation dose, 255 consecutive patients with low HR (≤60 bpm) and without arrhythmia underwent cardiac MDCT using high HP. We had already reported that the relationship among r, HP, and the maximum data acquisition time interval (Tmax) does not create the data deficit in arrhythmia. It was represented as Tmax=(69.88/HP-0.64) r; (equation 1). From equation 1, HP=69.88 r/(Tmax+0.64 r); (equation 2) was derived. We measured the maximum R-R interval (R-Rmax) on electrocardiogram (ECG) before multi detector row CT (MDCT) acquisition, and R-Rmax x 1.1 was calculated as Tmax in consideration of R-Rmax prolongation during MDCT acquisition. The HP of high HP acquisition was calculated from equation 2. In HR≤50 bpm, Heart Navi determined r: 0.35 sec/rot and HP: 9.8, and in 51 bpm≤HR≤66 bpm, r:0.35 sec/rot and HP: 11.2. HP of the high HP (16.4±1.2) was significantly (p<0.0001) higher than that of Heart Navi HP (10.9±0.6). The scanning time (6.5±0.6 sec) of high HP was significantly (p<0.0001) shorter than that of Heart Navi (9.0±0.8 sec), and the dose length product of high HP (675±185 mGy·cm) was significantly (p<0.0001) lower than that of Heart Navi (923±252 mGy·cm). The high HP could produce fine images in 251/255 patients. In conclusion, the high HP acquisition is useful for reduction of radiation dose and scanning time. (author)

Modelling, experimentation and simulation of a reversible HP/ORC unit to get a Positive Energy Building

DEFF Research Database (Denmark)

Dumont, Olivier; Carmo, Carolina; Quoilin, Sylvain

2015-01-01

This paper presents an innovative building comprising a heat pump connected to a solar roof and a geothermal heat exchanger. This unit is able to invert its cycle and operate as an Organic Rankine Cycle (ORC). The solar roof is producing large amount of heat throughout the year. This allows...... and fluid R134a shows promising performance with a net electrical energy produced over one year reaching 4030 kWh. Following that, a prototype has been built and has proven the feasibility of the technology. Finally, a simulation code including the building, the ground heat exchanger, the thermal energy...... storage, the solar roof and the reversible HP/ORC unit is developed and allows to perform a sensivity analysis. Annual results show that this technology leads to a Positive Energy Building....

Reactive Geochemical Transport Modeling of Concentrated AqueousSolutions: Supplement to TOUGHREACT User's Guide for the PitzerIon-Interaction Model

Energy Technology Data Exchange (ETDEWEB)

Zhang, Guoxiang; Spycher, Nicolas; Xu, Tianfu; Sonnenthal, Eric; Steefel , Carl

2006-12-15

In this report, we present: -- The Pitzer ion-interactiontheory and models -- Input file requirements for using the TOUGHREACTPitzer ion-interaction model and associated databases -- Run-time errormessages -- Verification test cases and application examples. For themain code structure, features, overall solution methods, description ofinput/output files for parameters other than those specific to theimplemented Pitzer model, and error messages, see the TOUGHREACT User'sGuide (Xu et al., 2005). The TOUGHREACT Pitzer version runs on aDEC-alpha architecture CPU, under OSF1 V5.1, with Compaq Digital FortranCompiler. The compiler run-time libraries are required for execution aswell as compilation. The code also runs on Intel Pentium IV andhigher-version CPU-based machines with Compaq Visual Fortran Compiler orIntel Fortran Compiler (integrated with the Microsoft DevelopmentEnvironment). The minimum hardware configuration should include 1 GB RAMand 1 GB (2 GB recommended) of available disk space.

Applications of p-hydroxyphenacyl (pHP) and coumarin-4-ylmethyl photoremovable protecting groups.

Science.gov (United States)

Givens, Richard S; Rubina, Marina; Wirz, Jakob

2012-03-01

Most applications of photoremovable protecting groups have used o-nitrobenzyl compounds and their (often commercially available) derivatives that, however, have several disadvantages. The focus of this review is on applications of the more recently developed title compounds, which are especially well suited for time-resolved biochemical and physiological investigations, because they release the caged substrates in high yield within a few nanoseconds or less. Together, these two chromophores cover the action spectrum for photorelease from >700 nm to 250 nm.

Symmetry-Free, p-Robust Equilibrated Error Indication for the hp-Version of the FEMin Nearly Incompressible Linear Elasticity

OpenAIRE

Dörsek, Philipp; Melenk, Jens M.

2017-01-01

We consider the extension of the p-robust equilibrated error estimator due to Braess, Pillwein and Schöberl to linear elasticity. We derive a formulation where the local mixed auxiliary problems do not require symmetry of the stresses. The resulting error estimator is p-robust, and the reliability estimate is also robust in the incompressible limit if quadratics are included in the approximation space. Extensions to other systems of linear second-order partial differential equations are discu...

Thermal Evolution of Juvenile Subduction Zones ' New Constraints from Lu-Hf Geochronology on HP oceanic rocks (Halilbaǧi, Central Anatolia)

Science.gov (United States)

Pourteau, Amaury; Scherer, Erik; Schmidt, Alexander; Bast, Rebecca

2015-04-01

The thermal structure of subduction zones plays a key role on mechanical and chemical processes taking place along the slab-mantle interface. Until now, changes through time of this thermal structure have been explored mostly by the means of numerical simulations. However, both "warm" (i.e., epidote-bearing), and "cold" (i.e., lawsonite-bearing) HP oceanic rocks have been reported in some fossil subduction complexes exposed at the Earth's surface (e.g., Franciscan Complex, California; Rio San Juan Complex, Hispañola; Halilbağı Unit, Central Anatolia). These a-priori "incompatible" rocks witness different thermal stages of ancient subduction zones and their study might provide complementary constraints to numerical models. To decipher the meaning of these contrasting metamorphic rocks in the Halilbağı Unit, we are carrying out Lu-Hf geochronology on garnet (grt) and lws from a variety of HP oceanic rocks, as well as the metamorphic sole of the overlying ophiolite. We selected five samples that are representative of the variety of metamorphic evolutions (i.e. peak conditions and P-T paths) encountered in this area. Preliminary analyses yielded 110 Ma (grt-hbl isochron) for a sub-ophiolitic grt amphibolite; 92 Ma (grt-omp) for an eclogite with prograde and retrograde ep; 90 Ma (grt-omp) for an eclogitic metabasite with prograde ep and retrograde ep+lws; 87 Ma (grt-gln) for a lws eclogite with prograde ep; and 86 Ma (grt-gln) for a blueschist with prograde and retrograde lws. These ages are mainly two-point isochrons. Further-refined data will be presented at the EGU General Assembly 2015, in Vienna. The consistent younging trend from "warm" to "cold" metamorphic rocks revealed by these first-order results points to metamorphic-sole formation during the initiation of intra-oceanic subduction at ~110 Ma, and subsequent cooling of the slab-mantle interface between 92 and 86 Ma. Therefore, the contrasting metamorphic evolutions encountered in the Halilbağı Unit

Maritime Domain Awareness: The Key to Maritime Security Operational Challenges and Technical Solutions

Science.gov (United States)

2006-06-01

series with the Philippines, Indonesia, Singapore, Malaysia , Brunei, and the United States. Another example of regional collaboration is the South East...computers to choose from producers such as Sony , Fujitsu, Compaq, Toshiba, Macintosh or a custom-built PC. The selection depends on factors such as

New determinations of gamma-ray line intensities of the E{sub p}=550 and 1747 keV resonances of the {sup 13}C(p,{gamma}){sup 14}N reaction

Energy Technology Data Exchange (ETDEWEB)

Kiener, J. E-mail: kiener@csnsm.in2p3.fr; Gros, M.; Tatischeff, V.; Attie, D.; Bailly, I.; Bauchet, A.; Chapuis, C.; Cordier, B.; Deloncle, I.; Porquet, M.G.; Schanne, S.; Sereville, N. de; Tauzin, G

2004-03-01

Gamma-ray angular distributions for the resonances at E{sub p}=550 and 1747 keV of the radiative capture reaction {sup 13}C(p,{gamma}){sup 14}N have been measured, using intense proton beams on isotopically pure {sup 13}C targets. Experimental gamma-ray spectra were obtained with three HP-Germanium detectors at four angles for E{sub p}=550 keV and six angles for E{sub p}=1747 keV in the range of 0-90 deg. with respect to the proton beam. From the data, relative intensities for the strongest transitions were extracted with an accuracy of typically 5%, making these resonances new useful gamma-ray standards for efficiency calibration in the energy range from E{sub {gamma}}=1.6-9 MeV. Gamma-ray branching ratios were obtained for several levels of {sup 14}N and are compared with literature values.

Shape Optimization of Impeller Blades for 15,000 HP Centrifugal Compressor Using Fluid Structural Interaction Analysis

International Nuclear Information System (INIS)

Kang, Hyun Su; Oh, Jeongsu; Han, Jeong Sam

2014-01-01

This paper discusses a one-way fluid structural interaction (FSI) analysis and shape optimization of the impeller blades for a 15,000 HP centrifugal compressor using the response surface method (RSM). Because both the aerodynamic performance and the structural safety of the impeller are affected by the shape of its blades, shape optimization is necessary using the FSI analysis, which includes a structural analysis for the induced fluid pressure and centrifugal force. The FSI analysis is performed in ANSYS Workbench: ANSYS CFX is used for the flow field and ANSYS Mechanical is used for the structural field. The response surfaces for the FSI results (efficiency, pressure ratio, maximum stress, etc.) generated based on the design of experiments (DOE) are used to find an optimal shape for the impeller blades, which provides the maximum aerodynamic performance subject to the structural safety constraints

Shape Optimization of Impeller Blades for 15,000 HP Centrifugal Compressor Using Fluid Structural Interaction Analysis

Energy Technology Data Exchange (ETDEWEB)

Kang, Hyun Su [Sungkyunkwan University, Suwon (Korea, Republic of); Oh, Jeongsu [Daejoo Machinery Co., Daegu (Korea, Republic of); Han, Jeong Sam [Andong National University, Andong (Korea, Republic of)

2014-06-15

This paper discusses a one-way fluid structural interaction (FSI) analysis and shape optimization of the impeller blades for a 15,000 HP centrifugal compressor using the response surface method (RSM). Because both the aerodynamic performance and the structural safety of the impeller are affected by the shape of its blades, shape optimization is necessary using the FSI analysis, which includes a structural analysis for the induced fluid pressure and centrifugal force. The FSI analysis is performed in ANSYS Workbench: ANSYS CFX is used for the flow field and ANSYS Mechanical is used for the structural field. The response surfaces for the FSI results (efficiency, pressure ratio, maximum stress, etc.) generated based on the design of experiments (DOE) are used to find an optimal shape for the impeller blades, which provides the maximum aerodynamic performance subject to the structural safety constraints.

New high pressure die cast magnesium alloy AM-HP2 for powertrain applications

Energy Technology Data Exchange (ETDEWEB)

Gibson, M.A. [Queensland Univ., Brisbane (Australia). CAST CRC]|[CSIRO Manufacturing and Materials Technology, Clayton (Australia); Zhu, S.M.; Nie, J.F. [Queensland Univ., Brisbane (Australia). CAST CRC]|[Monash Univ., Monash (Australia). Dept. of Materials Engineering

2007-07-01

In order to improve the benefits available through weight savings on engine emissions, magnesium alloys must gain wider acceptance in transmission and engine applications. This paper provided details of a new alloy with exceptional creep properties developed for automotive powertrain applications. The AM-HP2 alloy was developed as part of a broader research program investigating the relationship between the composition, structure and properties of magnesium rare earth (Mg-RE) based alloys and high-pressure die casting (HPDC). Cylindrical test specimens of various alloys were produced on a cold chamber HPDC machine. Tensile creep tests were then conducted under a constant load at 177 degrees C for a period of 600 hours. Microstructures of specimens were characterized using a CM20 transmission electron microscope. Results of the tests indicated that near-grain boundary microstructure was an important parameter in securing optimum elevated temperature properties. The RE element had a significant effect on the creep behaviour of the HPDC Mg-RE alloys. It was concluded that further research is needed to investigate the influence of the RE mixture in improving creep performance. 15 refs., 2 tabs., 6 figs.

Effects of Body Mass Index on Mechanical Properties of the Plantar Fascia and Heel Pad in Asymptomatic Participants.

Science.gov (United States)

Taş, Serkan; Bek, Nilgün; Ruhi Onur, Mehmet; Korkusuz, Feza

2017-07-01

Musculoskeletal foot disorders have a high incidence among overweight and obese individuals. One of the important factors causing this high incidence may be plantar fascia and heel pad (HP)-related mechanical changes occurring in these individuals. The aim of the present study was to investigate the plantar fascia and HP stiffness and thickness parameters in overweight and obese individuals and compare these values with those of normal-weight individuals. This study was carried out in 87 (52 female, 35 male) healthy sedentary individuals between the ages of 19 and 58 years (34 ± 11 years). Participants were subsequently categorized according to body mass index (BMI) as normal weight (18.5 kg/m 2 Plantar fascia and HP thickness and stiffness were measured with an ultrasonography device using a linear ultrasonography probe. Overweight and obese individuals had higher HP thickness ( P plantar fascia thickness ( P = .001), heel pad microchamber layer (MIC) stiffness ( P plantar fascia stiffness ( P plantar fascia thickness ( P = .001, r = 0.536), MIC stiffness ( P plantar fascia stiffness ( P plantar fascia and an increase in the thickness of the plantar fascia as well as the thickness and stiffness of HP. Increased body mass could cause changes in the mechanical properties of HP and plantar fascia. Level 3, comparative study.

Investigation of Trimethyllysine Binding by the HP1 Chromodomain via Unnatural Amino Acid Mutagenesis.

Science.gov (United States)

Baril, Stefanie A; Koenig, Amber L; Krone, Mackenzie W; Albanese, Katherine I; He, Cyndi Qixin; Lee, Ga Young; Houk, Kendall N; Waters, Marcey L; Brustad, Eric M

2017-12-06

Trimethyllysine (Kme3) reader proteins are targets for inhibition due to their role in mediating gene expression. Although all such reader proteins bind Kme3 in an aromatic cage, the driving force for binding may differ; some readers exhibit evidence for cation-π interactions whereas others do not. We report a general unnatural amino acid mutagenesis approach to quantify the contribution of individual tyrosines to cation binding using the HP1 chromodomain as a model system. We demonstrate that two tyrosines (Y24 and Y48) bind to a Kme3-histone tail peptide via cation-π interactions, but linear free energy trends suggest they do not contribute equally to binding. X-ray structures and computational analysis suggest that the distance and degree of contact between Tyr residues and Kme3 plays an important role in tuning cation-π-mediated Kme3 recognition. Although cation-π interactions have been studied in a number of proteins, this work is the first to utilize direct binding assays, X-ray crystallography, and modeling, to pinpoint factors that influence the magnitude of the individual cation-π interactions.

Modeling of Yield Estimation for The Main Crops in Iran Based on Mechanization Index (hp ha-1

Directory of Open Access Journals (Sweden)

K Abbasi

2014-09-01

Full Text Available Agricultural mechanization is a method for transiting from traditional agriculture towards industrial and sustainable one. Due to the limitation of natural resources and increasing population we need to have economical production of agricultural crops. For reaching this destination; agricultural mechanization has a remarkable role. So it is necessary to have an extensive view for mechanization, because with the help of mechanization the agricultural inputs such as seeds, fertilizer and even water and soil can effectively be managed for an economical and sustainable production. This study has been carried out in many provinces of Iran. The data of agricultural tractors and cereal combine harvesters were firstly gathered by means of questionnaire. The tractors were categorized in four power levels of less than 45, 45 to 80, 80 to 110, and more than 110 hp. In addition, it was also carried out for cereal combine harvesters; it was in three power levels, i.e. between 100 to 110, 110 to 155 and 155 to 210 horse-power in 3 ages, i.e. less than 13, between 13 to 20, and more than 20 years. Information regarding to cultivation areas, production volume, and yield of main crops gathered from statistics of Ministry of Jihad-e-Agriculture. Then agriculture mechanization level index (hp ha-1 in each province was calculated. Four main crops including irrigated and rain-fed wheat and irrigated and rain-fed barley, which met the required criteria to be used in the model, were statistically analyzed. Correlation analysis was carried out in order to get an effective model between yield of the four main crops in Iran and agriculture mechanization level index. Pearson correlation index showed that there is a direct and significant correlation between these variables. Subsequently, outliers were identified in order to get a model with necessary efficiency to predict the yield through mechanization level index, by scatter diagram and estimating regression lines in 1

Asociación entre gastritis folicular y Helicobacter pylori en niños atendidos en un hospital público peruano

Directory of Open Access Journals (Sweden)

C.R. Mejia

2016-04-01

Conclusiones: Sobre la base de los datos analizados se concluye que los niños con gastritis folicular tienen más probabilidad de tener Hp con respecto a los que no presentan gastritis. Los resultados pueden ser extrapolados a otras poblaciones similares, debiendo ser evaluado cada escenario para que esto no se convierta en un problema de salud pública en unos años.

Use of a low-background and anti-Compton HpGe gamma-spectrometer in analyses of environmental samples

Energy Technology Data Exchange (ETDEWEB)

Qiong, Su; Yamin, Gao [Ministry of Public Health, Beijing, BJ (China). Lab. of Industrial Hygiene

1989-12-01

The results of application of a HpGe gamma-spectrometer in the analyses of enviromental samples are reported. The spectrometer has very low background and good property of Compton suppression. By comparison between the gamma-spectra with and without anti-coincidence shield for the same samples, the advantage in analysing environmental samples became apparent. In the analyses of carp samples, the ratio of specific activities of {sup 226}Ra in the flesh and bone of the carp was 1 to 35, which is basically in agreement with the ratio of the accumulation factors 1:37, as reported in the literature. Thus the spectrometer would play an important role in the research of the transfer of radionuclides of low activity in the environment. The method of gamma-ray data processing is also described.

Use of a low-background and anti-Compton HpGe gamma-spectrometer in analyses of environmental samples

International Nuclear Information System (INIS)

Su Qiong; Gao Yamin

1989-01-01

The results of application of a HpGe gamma-spectrometer in the analyses of enviromental samples are reported. The spectrometer has very low background and good property of Compton suppression. By comparison between the gamma-spectra with and without anti-coincidence shield for the same samples, the advantage in analysing environmental samples became apparent. In the analyses of carp samples, the ratio of specific activities of 226 Ra in the flesh and bone of the carp was 1 to 35, which is basically in agreement with the ratio of the accumulation factors 1:37, as reported in the literature. Thus the spectrometer would play an important role in the research of the transfer of radionuclides of low activity in the environment. The method of gamma-ray data processing is also described

Soybean P34 Probable Thiol Protease Probably Has Proteolytic Activity on Oleosins.

Science.gov (United States)

Zhao, Luping; Kong, Xiangzhen; Zhang, Caimeng; Hua, Yufei; Chen, Yeming

2017-07-19

P34 probable thiol protease (P34) and Gly m Bd 30K (30K) show high relationship with the protease of 24 kDa oleosin of soybean oil bodies. In this study, 9 day germinated soybean was used to separate bioprocessed P34 (P32) from bioprocessed 30K (28K). Interestingly, P32 existed as dimer, whereas 28K existed as monomer; a P32-rich sample had proteolytic activity and high cleavage site specificity (Lys-Thr of 24 kDa oleosin), whereas a 28K-rich sample showed low proteolytic activity; the P32-rich sample contained one thiol protease. After mixing with purified oil bodies, all P32 dimers were dissociated and bound to 24 kDa oleosins to form P32-24 kDa oleosin complexes. By incubation, 24 kDa oleosin was preferentially hydrolyzed, and two hydrolyzed products (HPs; 17 and 7 kDa) were confirmed. After most of 24 kDa oleosin was hydrolyzed, some P32 existed as dimer, and the other as P32-17 kDa HP. It was suggested that P32 was the protease.

Characterization and human gingival fibroblasts biocompatibility of hydroxyapatite/PMMA nanocomposites for provisional dental implant restoration

Science.gov (United States)

Zhang, Jingchao; Liao, Juan; Mo, Anchun; Li, Yubao; Li, Jidong; Wang, Xuejiang

2008-11-01

The aim of this study was to determine nHA/PMMA composites (H/P) in an optimal ratio with improved cytocompatibility as well as valid physical properties for provisional dental implant restoration. 20 wt.%, 30 wt.%, 40 wt.% and 50 wt.% H/P were developed and characterized using XPS, bending strength test and SEM. Human gingival fibroblasts cultured in extracts or directly on sample discs were investigated by fluorescent staining and MTT assay. Chemical integration in nHA/PMMA interface was indicated by XPS. Typical fusiform cells with adhesion spots were detected on H/P discs. MTT results also indicated higher cell viability in 30 wt.% and 40 wt.% H/P discs ( P provisional fixed crowns (PFC) is 0.4:1.

Prion protein with Y145STOP mutation induces mitochondria-mediated apoptosis and PrP-containing deposits in vitro

International Nuclear Information System (INIS)

Hachiya, Naomi S.; Watanabe, Kota; Kawabata, Makiko Y.; Jozuka, Akiko; Kozuka, Yoshimichi; Sakasegawa, Yuji; Kaneko, Kiyotoshi

2005-01-01

A pathogenic truncation of an amber mutation at codon 145 (Y145STOP) in Gerstmann-Straussler-Scheinker disease (GSS) was investigated through the real-time imaging in living cells, by utilizing GFP-PrP constructs. GFP-PrP(1-144) exhibited an aberrant localization to mitochondria in mouse neuroblastoma neuro2a (N2a) and HpL3-4 cells, a hippocampal cell line established from prnp gene-ablated mice, whereas full-length GFP-PrP did not. The aberrant mitochondrial localization was also confirmed by Western blot analysis. Since GFP-PrP(1-121), as previously reported, and full-length GFP-PrP do not exhibit such mitochondrial localization, the mitochondrial localization of GFP-PrP(1-144) requires not only PrP residues 121-144 (in human sequence) but also COOH-terminal truncation in the current experimental condition. Subsequently, the GFP-PrP(1-144) induced a change in the mitochondrial innermembrane potential (ΔΨ m ), release of cytochrome c from the intermembrane space into the cytosol, and DNA fragmentation in these cells. Non-fluorescent PrP(1-144) also induced the DNA fragmentation in N2a and HpL3-4 cells after the proteasomal inhibition. These data may provide clues as to the molecular mechanism of the neurotoxic property of Y145STOP mutation. Furthermore, immunoelectron microscopy revealed numerous electron-dense deposits in mitochondria clusters of GFP-PrP(1-144)-transfected N2a cells, whereas no deposit was detected in the cells transfected with full-length GFP-PrP. Co-localization of GFP/PrP-immunogold particles with porin-immunogold particles as a mitochondrial marker was observed in such electron-dense vesicular foci, resembling those found in autophagic vacuoles forming secondary lysosomes. Whether such electron-dense deposits may serve as a seed for the growth of amyloid plaques, a characteristic feature of GSS with Y145STOP, awaits further investigations

A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.

Science.gov (United States)

Tamekou Lacmata, Stephen; Yao, Lan; Xian, Mo; Liu, Hui; Kuiate, Jules-Roger; Liu, Huizhou; Feng, Xinjun; Zhao, Guang

2017-09-03

The release of intracellular products, especially polyhydroxyalkanoates, is still a great challenge in industry. To solve this bottleneck, a novel autolysis system strictly controlled with magnesium was constructed and applied to poly(3-hydroxypropionate) production in engineered Escherichia coli. The autolysis system was constructed by inserting the 5'untranslated region (5'UTR) behind promoter PmgtA with lysis genes (S, R, and Rz, from E. coli) overexpressed. The autolysis system functioned well (lysis efficiency of more than 90%) in the P3HP producer with double plasmids containing lysis genes and P3HP biosynthesis genes, whereas the P3HP production was reduced due to plasmid losses. After the autolysis genes and P3HP biosynthesis genes were integrated into one plasmid, the P3HP content of 72.7% (2.4 times of the control) and the plasmid stability of 79.8 ± 3.1% were achieved in strain Q2646 with promoter PmgtA-UTR. However, the strain Q2647 with promoter PmgtA could not accumulate P3HP because of rapid cell lysis. The novel autolysis system activated in Mg 2+ -depleted conditions proves to be feasible for polyhydroxyalkanoates production, which may have great application potential for other intracellular products.

High-Pressure Spark Plasma Sintering (HP SPS): A Promising and Reliable Method for Preparing Ti-Al-Si Alloys.

Science.gov (United States)

Knaislová, Anna; Novák, Pavel; Cygan, Sławomir; Jaworska, Lucyna; Cabibbo, Marcello

2017-04-27

Ti-Al-Si alloys are prospective material for high-temperature applications. Due to low density, good mechanical properties, and oxidation resistance, these intermetallic alloys can be used in the aerospace and automobile industries. Ti-Al-Si alloys were prepared by powder metallurgy using reactive sintering, milling, and spark plasma sintering. One of the novel SPS techniques is high-pressure spark plasma sintering (HP SPS), which was tested in this work and applied to a Ti-10Al-20Si intermetallic alloy using a pressure of 6 GPa and temperatures ranging from 1318 K (1045 °C) to 1597 K (1324 °C). The low-porosity consolidated samples consist of Ti₅Si₃ silicides in an aluminide (TiAl) matrix. The hardness varied between 720 and 892 HV 5.

Software Aspects of IEEE Floating-Point Computations for Numerical Applications in High Energy Physics

CERN Multimedia

CERN. Geneva

2010-01-01

hazards to be avoided About the speaker Jeffrey M Arnold is a Senior Software Engineer in the Intel Compiler and Languages group at Intel Corporation. He has been part of the Digital->Compaq->Intel compiler organization for nearly 20 years; part of that time, he worked on both lo...

[Relationship between intestinal mucosal inflammation and mental disorders in patients with irritable bowel syndrome].

Science.gov (United States)

Hao, Jing-xin; Han, Mai; Duan, Li-ping; Han, Ya-jing; Ge, Ying; Huang, Yue-qin

2012-08-28

To examine the relationship between inflammation and the comorbidity of mental disorders with irritable bowel syndrome (IBS) by comparing intestinal mucosa inflammatory biomarkers in patients with and without mental disorders. A total of 43 consecutive IBS patients fulfilling the Rome III criteria and 15 volunteers serving as controls without digestive symptoms were recruited and interviewed with Composite International Diagnostic Interview (CIDI) by the well-trained staff and thus classified as with or without mental disorders. All subjects underwent colonoscopy and biopsies were acquired from the mucosa of distal ileum and colon. CD3(+) lymphocytes, mast cells, 5-HT positive cells and (indoleamine 2,3-dioxygenase) IDO positive cells were identified immunohistologically in mucosa biopsies in volunteers (n = 13), IBS patients without mental disorder (n = 24) and IBS patients with mental disorder (n = 19). The incidence of mental disorders in IBS patients was significantly higher than that in the volunteers (19/43 vs 2/15, P = 0.012), including 9 patients with anxiety disorders and 8 with mood disorders. (1) The number of mast cells in IBS patients with mental disorder and that in IBS patients without mental disorder has no statistical significance ((16.7 ± 3.6)/HP vs (15.4 ± 3.1)/HP in distal ileum, (12.8 ± 2.2)/HP vs (12.3 ± 2.5)/HP in sigmoid, both P > 0.05). Similar results were seen in 5-HT positive cells ((3.7 ± 0.9)/HP vs (3.4 ± 0.8)/HP in distal ileum, (6.1 ± 1.8)/HP vs (5.2 ± 1.8)/HP in sigmoid, both P > 0.05). In distal ileum, the number of CD3(+) cells in IBS patients with mental disorder has no statistical significance with that in the IBS patients without mental disorder ((62 ± 16)/HP vs (55 ± 22)/HP, P > 0.05). Similar results were seen in IDO positive cells (6(2, 8)/HP vs 2(1, 5)/HP, P > 0.05). (2) The number of IDO positive cells from distal ileum in IBS patients with anxiety disorder was significantly higher than that in the IBS patients

Stability estimates for h-p spectral element methods for elliptic ...

Indian Academy of Sciences (India)

R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

With this mesh we seek a solution which minimizes a weighted ... uniform mesh; fractional Sobolev norms; stability estimate; polylogarithmic bounds. ... In [6,7] we restrict ourselves to examining the Poisson's equation with ... it by stating the stability theorem 3.1 for a simpler case. ..... And this gives us the following inequality:.

Stability estimates for h-p spectral element methods for general ...

Indian Academy of Sciences (India)

R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

Moreover let the bilinear form induced by the operator L satisfy the inf–sup conditions. ..... space of polynomials of degree W in ξ and η with respect to the usual inner product in. H2(S). ..... Let us define the new differential operator ...... The research by PD is partly supported by Aeronautical Research and Development Board.

High pressure 129I Moessbauer studies of GeI4 molecular crystals

International Nuclear Information System (INIS)

Pasternak, M.P.; Taylor, R.D.

1989-01-01

The Moessbauer effect in 129 I in conjunction with Diamond-Anvil-Cell high pressure techniques was applied to investigate the high pressure phase(s) of the molecular crystal GeI 4 . The 129 I Quadrupole Interaction was the main probe for characterizing the intermolecular structural transformation with pressure. With increasing pressure, at about 15 GPAa, the onset of a partial molecular-association phase (HP1) is first observed. In HP1 two out of the four iodines strongly overlap to form linear chains of GeI 4 . The HP1 phase coexists with the low pressure (LP) molecular phase, but its population increases with increasing pressure. At P ∼20 GPa a second high pressure phase (HP2) is identified where all four iodines strongly overlap to form a three dimensional, fully molecular-associated structure. With increasing pressure and at P > 20 GPa, HP2 is the only phase up to P = 34 GPa, the highest pressure used. A significant hysteresis of the relative abundances with pressure is observed. The isomer shift of the HP2 and HP1 structures is considerably larger than that of the LP one. 11 refs., 3 figs

Synthesis, vibrational characteristic and luminescence properties of Er3+in HNH4ErP3O10

Science.gov (United States)

Chékir-Mzali, Jalila; Elgharbi, Sarra; Horchani-Naifer, Karima; Férid, Mokhtar

2017-11-01

Polycrystalline powders of the triphosphate of ammonium hydrogen and erbium NH4ErHP3O10 are grown by flux method. The obtained sample was characterized by X-Ray Diffraction, Infrared and Raman spectroscopies. It was found that this compound is isostructural with NH4BiHP3O10. It crystallizes in the triclinic system, with space group P-1. The spectroscopic properties have been investigated in the UV-Vis and IR region via absorption, excitation, and emission spectra at room temperature. Also, the particle size distribution was investigated and the average crystallite size of HNH4ErP3O10 was 124.08 nm. The optical band gap energy (Eg ≈ 4.24 eV) was calculated from the diffuse reflectance by using the Kubelka-Munk function and Tauc's relation. The time-resolved luminescence of 4F9/2 emission has also been studied. The decay time was shown to be exponential with a pure radiative transition and the lifetime value for the transition 4F9/2 → 4I15/2 is found equal to 15, 4 μs. The effects of the crystal structure, NH4+ and OH groups on the lifetime have been revealed. The CIE chromaticity coordinates reveal that this phosphor can be a promising Yellow-orange emitting material.

SEASONAL VARIATIONS IN AIR POLLUTION PARTICLE-INDUCED INFLAMMATORY MEDIATOR RELEASE AND OXIDATIVE STRESS

Science.gov (United States)

Normal human bronchial epithelial (NHBE) cells and alveolar macrophages (AMs) were exposed to equal mass of coarse [PM with aerodynamic diameter of 2.510 �m (PM2.510)], fine (PM2.5), and ultrafine (PM < 0.1) ambient PM from Chapel Hill, North Carolina, during October 2001 (f...

CERN is first to demonstrate a 10 Gigabit/sec network

CERN Multimedia

CERN Press Office. Geneva

2000-01-01

CERN* has made another important step forward in high-speed computing and state of the art networking. On 15 September a Gigabyte System Network (GSN) network with a capacity of 10 Gbit per second ramped up successfully at CERN, connecting together computers from different computer companies (Compaq, IBM, SGI and Sun).

The Prevalence of Helicobacter Pylori in Dyspeptic Patients and the Relationship with Endoscopic Diagnosis

Directory of Open Access Journals (Sweden)

Ozgur Turk

2016-07-01

Full Text Available Aim: One of the most common reasons of the chronic gastritis is Helicobacter pylori (Hp infection. Recent studies reported that Hp gastritis is involved with the duodenal ulcer, atrophic gastritis, dyspepsia, gastric cancer and MALT lymphoma. The prevalence of Hp infection has regional differences. We aimed to determine the frequency of Hp infection in our region and introduce the relationship between results of endoscopy with Hp infection. Material and Method: In this study we analyzed retrospectively 412 patients%u2019 endoscopy results and histopathological results between 2013 January-2014 February.Total 246 patients who have underwent endoscopic biopsy and Hphistopathologically investigated. Results: Male and female ratio was 1.8:1. There were 77 male patients and 139 female patients. Mean age of patients was 46.50±15.05 and range of age was 15 to 82.There was not any statically meaningful association between Hp and patients ages and gender (p>0.05.47.7% of patients were diagnosed as Hp positive, There was a meaningful relationship between Hp ( and chronic gastritis ( groups (p

Remeasurement of p/suby/ for the 2H(d, n)3He reaction and its bearing on the reported f-wave admixture of the lowest 2- state in 4He

International Nuclear Information System (INIS)

Tornow, W.; Skubic, S.E.; Byrd, R.C.; Lisowski, P.W.; Walter, R.L.

1976-01-01

The polarization of neutrons from the reaction 2 H(d, n) 3 He has been measured for a deuteron energy E/subd/ = 2.44 MeV at emission angles of 45degree and 55degree (lab). The polarization values are discussed in relation both to previously reported discrepant data and to measured differences between p/suby/ and A/suby/ in the reaction 3 H(p, n) 3 He

Nodular gastritis: an endoscopic indicator of Helicobacter pylori infection in children.

Science.gov (United States)

Mazigh Mrad, Sonia; Abidi, Kamel; Brini, Ines; Boukthir, Samir; Sammoud, Azza

2012-11-01

Helicobacter pylori (Hp) infection is a common and universally distributed bacterial infection. It is predominantly acquired in childhood. To assess the relationship between endoscopic nodular gastritis and Hp infection. A retrospective study was conducted in children who underwent upper gastrointestinal endoscopy for chronic abdominal pain. All children who had five gastric biopsies for histologic analysis, urease test and Hp culture were included in the study. The Sensitivity and sensibility of nodular gastritis were determined and different parameters were studied according the presence or not of nodular gastritis(age, gender, Hp status, gastritis score and lymphoid follicles). 49 children, mean age 6.9 ± 3 years (range 1-12 years) were eligible for the analysis. Hp Infection was diagnosed in 35 out of the 49 children (71.5 %). Nodular gastritis was recorded in 16 out of 49 children and in 14 infected children and 2 out of 14 non infected children (p=0.07), 40% sensitivity, 85.7% specificity, 87.5% positive predictive value, and 36,4% negative predictive value. The parameters associated significantly to the presence of nodular gastritis were older age: (p=0.04), Hp infection: (p=0.01), chronic gastritis: (p=0.05), active gastritis: (p=0.02), follicular gastritis: (p=0.005), higher gastritis score: (p=0.005). Completely normal gastric mucosal histology was never found in infected children with antral nodularity. Among all infected children, the gastritis score was significantly higher when there was a nodular gastritis and follicular gastritis was significantly associated to nodular gastritis. The endoscopic pattern of antral nodularity identifies children with Hp infection, and active chronic follicular gastritis.

Meals based on vegetable protein sources (beans and peas are more satiating than meals based on animal protein sources (veal and pork – a randomized cross-over meal test study

Directory of Open Access Journals (Sweden)

Marlene D. Kristensen

2016-10-01

Full Text Available Background: Recent nutrition recommendations advocate a reduction in protein from animal sources (pork, beef because of environmental concerns. Instead, protein from vegetable sources (beans, peas should be increased. However, little is known about the effect of these vegetable protein sources on appetite regulation. Objective: To examine whether meals based on vegetable protein sources (beans/peas are comparable to meals based on animal protein sources (veal/pork regarding meal-induced appetite sensations. Design: In total, 43 healthy, normal-weight, young men completed this randomized, double-blind, placebo-controlled, three-way, cross-over meal test. The meals (all 3.5 MJ, 28 energy-% (E% fat were either high protein based on veal and pork meat, HP-Meat (19 E% protein, 53 E% carbohydrate, 6 g fiber/100 g; high protein based on legumes (beans and peas, HP-Legume (19 E% protein, 53 E% carbohydrate, 25 g fiber/100 g; or low-protein based on legumes, LP-Legume (9 E% protein, 62 E% carbohydrate, 10 g fiber/100 g. Subjective appetite sensations were recorded at baseline and every half hour using visual analog scales until the ad libitum meal 3 h after the test meal. Repeated measurements analyses and summary analyses were performed using ANCOVA (SAS. Results: HP-Legume induced lower composite appetite score, hunger, prospective food consumption, and higher fullness compared to HP-Meat and LP-Legume (p<0.05. Furthermore, satiety was higher after HP-Legume than HP-Meat (p<0.05. When adjusting for palatability, HP-Legume still resulted in lower composite appetite scores, hunger, prospective consumption, and higher fullness compared to HP-Meat (p<0.05. Furthermore, HP-Legume induced higher fullness than LP-Legume (p<0.05. A 12% and 13% lower energy intake, respectively, was seen after HP-Legume compared to HP-Meat or LP-Legume (p<0.01. Conclusion: Vegetable-based meals (beans/peas influenced appetite sensations favorably compared to animal-based meals

Progression of emphysema evaluated by MRI using hyperpolarized (3)He (HP (3)He) measurements in patients with alpha-1-antitrypsin (A1AT) deficiency compared with CT and lung function tests

DEFF Research Database (Denmark)

Stavngaard, T; Søgaard, L Vejby; Batz, M

2009-01-01

BACKGROUND: The progression of emphysema is traditionally measured by pulmonary function test, with forced expiratory volume in 1 s (FEV(1)) being the most accepted and used measurement. However, FEV(1) is insensitive in detecting mild/slow progression of emphysema because of low reproducibility...... as compared to yearly decline. PURPOSE: To investigate the progression of emphysema over a period of 2 years using diffusion-weighted hyperpolarized (HP) (3)He magnetic resonance imaging (MRI) in patients with alpha-1-antitrypsin (A1AT) deficiency. MATERIAL AND METHODS: Nine patients with severe A1AT...... density [CT-PD15] and relative area of emphysema below -910 HU [CT-RA-910]). RESULTS: Seven patients were scanned three times, one patient two times, and one patient only at baseline. The mean increase in ADC values from first to last HP (3)He MR scanning was 3.8% (0.014 cm(2)/s [SD 0.024 cm(2)/s...

Dual photon absorptiometer utilizing a HpGe detector and microprocessor controller

International Nuclear Information System (INIS)

Ellis, K.J.; Vartsky, D.; Pearlstein, T.B.; Alberi, J.L.; Cohn, S.H.

1978-01-01

The analysis of bone mineral content (BMC) using a single energy-photon beam assumes that there are only two materials present, bone mineral and a uniform soft tissue component. Uncertainty in the value of BMC increases with different adipose tissue components in the transmitted beam. These errors, however, are reduced by the dual energy technique. Also, extension to additional energies further identifies the separate constituents of the soft tissue component. A multi-energy bone scanning apparatus with data acquisition and analysis capability sufficient to perform multi-energy analysis of bone mineral content was designed and developed. The present work reports on the development of device operated in the dual energy mode. The high purity germanium (HpGe) detector is an integral component of the scanner. Errors in BMC due to multiple small angle scatters are reduced due to the excellent energy resolution of the detector (530 eV at 60 keV). Also, the need to filter the source or additional collimation on the detector is eliminated. A new dual source holder was designed using 200 mCi 125 I and 100 mCi 241 Am. The active areas of the two source capsules are aligned on a common axis. The congruence of the dual source was verified by measuring the collimator response function. This new holder design insures that the same tissue mass simultaneously attenuates both sources. The controller portion of the microprocessor allows for variation in total scan length, step size, and counting time per step. These options allow for multiple measurements without changes in the detector, source, or collimator. The system has been successfully used to determine the BMC content of different bones

Corrosion by sulfate-reducing bacteria in a HP gas line under a detached weld cladding; Korrosion durch sulfatreduzierende Bakterien an einer Hochdruckgasleitung unter abgeloester Schweissnahtnachumhuellung

Energy Technology Data Exchange (ETDEWEB)

Bette, Ulrich [Technische Akademie Wuppertal (Germany)

2011-07-01

Intelligent pig measurements detected several points of corrosion in a HP gas pipeline in northern Germany. Corrosion occurred in a pipe section buried in clay soil, under detached weld claddings. It was not detected in regular measurements and additional intensive measurements. When the pipes were dug up, sulfate-reducing bacteria were found as the cause of corrosion. Due to the location of the corrosion processes, cathodic protection was impossible, and IFO measurements were ineffective in the low-ohmic soil.

Helicobacter pylori Infection and Insulin Resistance in Diabetic and Nondiabetic Population

Directory of Open Access Journals (Sweden)

Jamshid Vafaeimanesh

2014-01-01

Full Text Available Helicobacter pylori (HP is a common worldwide infection with known gastrointestinal and nongastrointestinal complications. One of the gastrointestinal side effects posed for this organism is its role in diabetes and increased insulin resistance. The aim of this study was to evaluate the association between HP and insulin resistance in type 2 diabetic patients and nondiabetics. This cross-sectional study was carried out from May to December 2013 on 211 diabetic patients referred to diabetes clinic of Shahid Beheshti Hospital of Qom and 218 patients without diabetes. HP was evaluated using serology method and insulin resistance was calculated using HOMA-IR. The prevalence of H. pylori infection was 55.8% and 44.2% in diabetics and nondiabetics (P=0.001. The study population was divided into two HP positive and negative groups. Among nondiabetics, insulin resistance degree was 3.01±2.12 and 2.74±2.18 in HP+ and HP− patients, respectively P=0.704. Oppositely, insulin resistance was significantly higher in diabetic HP+ patients rather than seronegative ones (4.484±2.781 versus 3.160±2.327, P=0.013. In diabetic patients, in addition to higher prevalence of HP, it causes a higher degree of insulin resistance.

Efficacy of low-calorie, partial meal replacement diet plans on weight and abdominal fat in obese subjects with metabolic syndrome: a double-blind, randomised controlled trial of two diet plans - one high in protein and one nutritionally balanced.

Science.gov (United States)

Lee, K; Lee, J; Bae, W K; Choi, J K; Kim, H J; Cho, B

2009-02-01

Little is known about the relative efficacy of high-protein vs. conventional diet plans that include partial meal replacements on body fat loss in obese subjects with metabolic syndrome. We aimed to evaluate the efficacy of two low-calorie diets with partial meal replacement plans-a high-protein plan (HP) and a nutritionally balanced conventional (C) plan-on reducing obesity in obese subjects with metabolic syndrome. In a 12-week, double-blind study, we randomised 75 participants to either the HP- or the C-plan group. We recorded key metrics at 0 and 12 weeks. The overall mean weight loss was 5 kg in the HP-plan group and 4.9 kg in the C-plan group (p = 0.72). Truncal fat mass decreased 1.6 kg in the HP-plan group (p or = 70% dietary compliance, however, truncal and whole body fat mass decreased more in the HP-plan group (Delta 2.2 kg and Delta 3.5 kg respectively) than in the C-plan group (Delta 1.3 kg and Delta 2.3 [corrected] kg respectively) (p < 0.05). The HP- and C-plans had a similar effect on weight and abdominal fat reduction, but the HP-plan was more effective in reducing body fat among compliant subjects.

Genetic, epidemiological, and clinical aspects of hereditary pancreatitis: a population-based cohort study in Denmark

DEFF Research Database (Denmark)

Brusgaard, Klaus

2010-01-01

, respectively, and among tIP patients 9 and 12%, respectively. Pancreatic cancer was diagnosed in 5% of the HP families. CONCLUSIONS: The genotype of the Danish population with HP differs from that of previously described cohorts. The occurrence of exocrine and endocrine insufficiency is higher among patients......-degree relatives of the 18 initially identified HP patients, 38 HP patients in total were identified, and 28 patients had SPINK1-CFTR mutations. Among HP patients, no p.N29I mutations were found and the p.A16V mutation was more frequent than previously reported, 45 and 32% had exocrine and endocrine insufficiency......OBJECTIVES: In a population-based, well-defined group of patients first regarded as having pancreatitis of unknown origin (PUO), we identified, described, and compared the clinical and genetic aspects of patients with hereditary pancreatitis (HP) and with cystic fibrosis transmembrane conductance...

Bleaching Gels Containing Calcium and Fluoride: Effect on Enamel Erosion Susceptibility

Directory of Open Access Journals (Sweden)

Alessandra B. Borges

2012-01-01

Full Text Available This in vitro study evaluated the effect of 35% hydrogen peroxide (HP bleaching gel modified or not by the addition of calcium and fluoride on enamel susceptibility to erosion. Bovine enamel samples (3 mm in diameter were divided into four groups (n=15 according to the bleaching agent: control—without bleaching (C; 35% hydrogen peroxide (HP; 35% HP with the addition of 2% calcium gluconate (HP + Ca; 35% HP with the addition of 0.6% sodium fluoride (HP + F. The bleaching gels were applied on the enamel surface for 40 min, and the specimens were subjected to erosive challenge with Sprite Zero and remineralization with artificial saliva for 5 days. Enamel wear was assessed using profilometry. The data were analyzed by ANOVA/ Tukey’s test (P<0.05. There were significant differences among the groups (P=0.009. The most enamel wear was seen for C (3.37±0.80 μm, followed by HP (2.89 ± 0.98 μm and HP + F (2.72 ± 0.64 μm. HP + Ca (2.31 ± 0.92 μm was the only group able to significantly reduce enamel erosion compared to C. The application of HP bleaching agent did not increase the enamel susceptibility to erosion. However, the addition of calcium gluconate to the HP gel resulted in reduced susceptibility of the enamel to erosion.

High-pressure treatment for shelf-life extension and quality improvement of oysters cooked in a traditional Taiwanese oyster omelet.

Science.gov (United States)

Lai, Kung-Ming; Chi, Hsin-Yi; Hsu, Kuo-Chiang

2010-01-01

Whole oysters were processed using high-pressure (HP) treatment at 250 and 300 MPa for 0 to 10 min and stored at 4 degrees Celsius for up to 28 days. HP-treated oysters and untreated oysters were evaluated for lipid oxidation, growth of microorganisms, and sensory characteristics after cooking at 160 degrees Celsius for 90 s. Microbial counts after HP treatment revealed that the bacterial load was initially reduced at all pressures. HP-treated oysters had significantly higher pH and moisture (P oysters during storage. HP treatment increased lipid oxidation with unpleasant odor during storage compared with the control. HP treatment decreased redness but did not significantly affect the brightness and yellowness of cooked oysters. From tests of mechanical properties, 300 MPa-treated oysters after cooking had significantly increased toughness as measured by cutting force. HP-treated oysters after cooking received higher quality scores than did the control during the storage trial. Results indicated that 300 MPa for 2 min is the optimum HP treatment that results in oysters most acceptable for oyster omelets during storage at 4 degrees Celsius, and this treatment may extend the shelf life of these oysters to 21 days.

High pressure sintering (HP-HT) of diamond powders with titanium and titanium carbide

International Nuclear Information System (INIS)

Jaworska, L.

1999-01-01

Polycrystalline diamond compacts for cutting tools are mostly manufactured using high pressure sintering (HP-HT). The standard diamond compacts are prepared by diamond powders sintering with metallic binding phase. The first group of metallic binder are metals able to solve carbon - Co, Ni. The second group of metal binders are carbide forming elements - Ti, Cr, W and others. The paper describes high pressure sintering of diamond powder with titanium and nonstoichiometry titanium carbide for cutting tool application. A type of binding phase has the significant influence on microstructure and mechanical properties of diamond compacts. Very homogeneous structure was achieved in case of compacts obtained from metalized diamond where diamond-TiC-diamond connection were predominant. In the case of compacts prepared by mechanical mixing of diamond with titanium powders the obtained structure was nonhomogeneous with titanium carbide clusters. They had more diamond to diamond connections. These compacts compared to the compact made of metallized diamond have greater wear resistance. In the case of the diamond and TiC 0.92 sintering the strong bonding of TiC diamond grains was obtained. The microstructure observations for diamond with 5% wt. Ti and diamond with 5% wt. TiC 0.92 (the initial composition) compacts were performed in transmission microscope. For two type of compacts the strong bonding phase TiC without defects is creating. (author)

Identification of aerosol particle sources in semi-rural area of Kwabenya, near Accra, Ghana, by EDXRF techniques

DEFF Research Database (Denmark)

Aboh, Innocent Joy Kwame; Henriksson, Dag; Laursen, Jens

2009-01-01

is frequently exposed to Harmattan dust from the Sahara-Sahel region. In total 171 filters each of PM2.5 and PM(2.5-10) were collected during 1 year. Levels of elements, black carbon (BC) and mass, were determined for both particle sizes. Principal component analysis (PCA) was performed on the datasets from...... Harmattan and non-Harmattan periods. The daily average of PM10 was very high, 179 μgm-3 and the BC contents were 4 μgm-3. The presence of crustal elements was large in PM(2.5-10) as well as in PM2.5, and had amore than tenfold increase in PM(2.5-10) during the Harmattan period. Major characteristic elements...

Oxonium ions substituting cesium ions in the structure of the new high-pressure borate HP-Cs{sub 1-x}(H{sub 3}O){sub x}B{sub 3}O{sub 5} (x=0.5-0.7)

Energy Technology Data Exchange (ETDEWEB)

Sohr, Gerhard; Heymann, Gunter; Wurst, Klaus; Huppertz, Hubert [Institut fuer Allgemeine, Anorganische und Theoretische Chemie, Leopold-Franzens-Universitaet Innsbruck (Austria); Neumair, Stephanie C. [Tyrolit Schleifmittelwerke Swarovski K.G., Schwaz (Austria); Schmedt auf der Guenne, Joern [Department fuer Chemie und Biologie, Universitaet Siegen (Germany)

2014-04-07

The new high-pressure borate HP-Cs{sub 1-x}(H{sub 3}O){sub x}B{sub 3}O{sub 5} (x=0.5-0.7) was synthesized under high-pressure/high-temperature conditions of 6 GPa/900 C in a Walker-type multianvil apparatus. The compound crystallizes in the monoclinic space group C2/c (Z=8) with the parameters a=1000.6(2), b=887.8(2), c=926.3(2) pm, β=103.1(1) , V=0.8016(3) nm{sup 3}, R1=0.0452, and wR2=0.0721 (all data). The boron-oxygen network is analogous to those of the compounds HP-MB{sub 3}O{sub 5}, (M=K, Rb) and exhibits all three structural motifs of borates - BO{sub 3} groups, corner-sharing BO{sub 4} tetrahedra, and edge-sharing BO{sub 4} tetrahedra - at the same time. Channels inside the boron-oxygen framework contain the cesium and oxonium ions, which are disordered on a specific site. Estimating the amount of hydrogen by solid-state NMR spectroscopy and X-ray diffraction led to the composition HP-Cs{sub 1-x}(H{sub 3}O){sub x}B{sub 3}O{sub 5} (x=0.5-0.7), which implies a nonzero phase width. (copyright 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

Association of Helicobacter pylori infection with serum vitamin B12 level in women of gestational age in Khorram Abad

Directory of Open Access Journals (Sweden)

fatemeh Yari

2006-02-01

Findings: There was no significant difference between mean age of HP positive and HP negative participants. 76 out of 110 (72.2% samples were HP positive. The rate of HP seropositivity was significantly higher in patients with serum vitamin B12 level less than 12 pg/ml. who had lower and moderate vitamin B12 levels (145-180 pg/ml, that is 94% vs 64.5%, P<0.013. Conclusion: High HP seropositivity rate among patients with low-normal less serum vitamin B12 levels than normal indicates that there may be an association between HP infection and serum vitamin B12 levels.

Mechanisms and kinetics of proliferation and fibrosis development in a mouse model of thyrocyte hyperplasia.

Science.gov (United States)

Ciornei, Radu Tudor; Hong, So-Hee; Fang, Yujiang; Zhu, Ziwen; Braley-Mullen, Helen

2016-01-01

IFN-γ(-/-) NOD.H-2h4 mice develop autoimmune disease with extensive hyperplasia and proliferation of thyroid epithelial cells (TEC H/P) and fibrosis. Splenic T cells from donors with severe TEC H/P transfer TEC H/P to SCID recipients. The goal of this study was to determine what factors control TEC H/P development/progression by examining T cells, markers of apoptosis, senescence and proliferation in thyroids of SCID recipients over time. At 28days, T cell infiltration was maximal, thyrocytes were proliferating, and fibrosis was moderate. At days 60 and 90, thyroids were larger with more fibrosis. T cells, cytokines and thyrocyte proliferation decreased, and cell cycle inhibitor proteins, and anti-apoptotic molecules increased. T cells and thyrocytes had foci of phosphorylated histone protein H2A.X, indicative of cellular senescence, when TEC H/P progressed and thyrocyte proliferation declined. Some thyrocytes were regenerating at day 90, with irregularly shaped empty follicles and ciliated epithelium. Proliferating thyrocytes were thyroid transcription factor (TTF1)-positive, suggesting they derived from epithelial cells and not brachial cleft remnants. Copyright © 2016 Elsevier Inc. All rights reserved.

Unraveling P-T-t-D Evolution of Zermatt-Saas Ophiolites from Valtournanche: from Ocean Opening to Mountain Building

Science.gov (United States)

Rebay, G.; Tiepolo, M.; Zanoni, D.; Langone, A.; Spalla, M. I.

2015-12-01

The Zermatt-Saas (ZS) Zone, formerly part of Tethyan oceanic crust and variously affected by oceanic metamorphism, is now part of the orogenic suture that developed in the Western European Alps during the Alpine subduction and collision. The ZS rocks preserve a dominant HP to UHP metamorphic imprint overprinted by greenschist facies metamorphism. The age of the oceanic protoliths is considered to be middle to upper Jurassic whereas the HP metamorphism is mostly considered to be Eocene. In upper Valtournanche ZS ophiolites, the dominant regional S2 foliation is mapped with spatial continuity in serpentinite, metarodingite and eclogite and is defined by HP/UHP parageneses in all lithotypes. It developed at 2.5 ± 0.3 GPa and 600 ± 20°C during Alpine subduction. S2 foliation of serpentinites wraps rare clinopyroxene and zircon relics. Trace element composition of clinopyroxene suggests that they crystallised from a melt in equilibrium with plagioclase: they most likely represent relicts of gabbroic assemblages. The clinopyroxene porphyroclasts have rims indented within S2 and compositions similar to fine-grained clinopyroxeneII defining S2, suggesting that they recrystallised during Alpine subduction. Zircon cores show, under CL, sector zoning typical of magmatic growth. U-Pb dates suggest their crystallisation during Middle Jurassic. Magmatic cores have thin fringe overgrowths parallel to the S2 foliation. U-Pb concordant analyses on these domains reveal an Upper Cretaceous-Paleocene crystallization most likely representing the HP to UHP Alpine re-equilibration. This suggests that some sections of the ZS have experienced HP to UHP metamorphism earlier than previously thought, opening new interpretative geodynamic scenarios. Remarkably, these new dates are similar to those recorded for the HP re-equilibration in the continental crust of the adjacent Austroalpine units (upper plate of the Alpine subduction system) and to those recorded for prograde metamorphism in

Long term effect of reduced dietary phosphorus on feed intake and milk yield in dry and lactating dairy cows

DEFF Research Database (Denmark)

Puggaard, Liselotte; Lund, Peter; Liesegang, A.

2014-01-01

Eighteen multiparous Holstein cows were used to study the long term effect of reducing dietary P concentration on intake of DM and milk yield, on blood levels of inorganic phosphate (Pi), Ca, vitamin D3, parathyroid hormone (PTH) as well as assessing bone turnover by the use of bone formation......) (2.1g P/kg DM) and High P (HP) (2.5g P/kg DM). After parturition and throughout lactation treatments were LP (2.3g P/kg DM), MP (2.8g P/kg DM) and HP (3.4g P/kg DM). Differences in dietary P were obtained by adding 0.60 and 1.19% mono-sodium phosphate per kg DM to MP and HP, respectively. The cows...... were fed restrictively pre-partum and for ad libitum intake from one week before expected calving and throughout the experiment. Due to a high number of health problems, LP treatment was terminated after sampling in week 12, and cows were shifted to HP and continued on this treatment until week 36...

Reduction of operational amplifiers finite gain effects in switched-capacitor biquads

Directory of Open Access Journals (Sweden)

Radev Nikolay

2005-01-01

Full Text Available A combined approach for reducing the errors in the pole frequency f p, the pole Q - factor Qp and the magnitude at the pole frequency Hp, of switched capacitor biquads is presented. First, the conventional integrators in the biquads are replaced with gain-and offset-compensated integrators. Next, the errors Δ ƒp / ƒp, Δ Qp / Qp and Δ Hp / Hp are minimized by modifying three capacitances: two feedback capacitances and feed forward capacitance. The effectiveness of this approach is demonstrated by designing a band pass biquad.

Cinesioterapia previne ombro doloroso em pacientes hemiplégicos/paréticos na fase sub-aguda do acidente vascular encefálico

Directory of Open Access Journals (Sweden)

Horn Agnes Irna

2003-01-01

Full Text Available CONTEXTO: Ombro doloroso é freqüente em pacientes com hemiplegia/hemiparesia (H/P por acidente vascular encefálico (AVE, dificultando a recuperação neuromotora gerando incapacidade funcional. OBJETIVO: Estudar tratamento fisioterapêutico para a prevenção da dor no ombro com H/P em pacientes com AVE na fase sub-aguda, e analisar desfechos secundários (força muscular do ombro acometido e movimentos funcionais ativos básicos. MÉTODO: Estudaram-se 21 pacientes (12 homens, 9 mulheres; idades 26 a 87 anos com H/P. O tratamento fisioterapêutico consistiu de 30 minutos diários de cinesioterapia, desde as 48 horas após o AVE até a alta hospitalar. Os pacientes foram avaliados antes e após o tratamento em relação à presença ou ausência de dor no ombro H/P, à força dos diversos grupos musculares do ombro e quanto aos movimentos funcionais de transferência e manutenção postural básica. RESULTADOS: Nenhum paciente apresentava dor no ombro H/P na alta (p<0,001. A força muscular aumentou significativamente em relação à elevação, protusão, abdução e flexão do ombro (p<0,001. Houve melhora dos movimentos funcionais: decúbito dorsal para lateral, decúbito lateral para sentado e manter-se sentado (p<0,001. CONCLUSÃO: A cinesioterapia na fase aguda do AVE preveniu a dor no ombro H/P e favoreceu a recuperação motora.

Hypoalbuminemia is an important risk factor of hypotension during hemodialysis.

Science.gov (United States)

Nakamoto, Hidetomo; Honda, Nobuko; Mimura, Taku; Suzuki, Hiromichi

2006-10-01

Hypotension during hemodialysis (HD) is an important problem in patients on HD. To investigate the risk factors that contribute to the hypotension during HD, we compared background factors of hypotensive (HP) patients during HD. Among 58 patients undergoing HD in Tamura Memorial Hospital, 12 patients could not continue full HD because of hypotension. We compared the data of ultrafiltration volume, cardiothoracic ratio (CTR), total protein (TP), serum albumin, blood urea nitrogen (BUN), serum creatinine, total cholesterol (TC), hemoglobin (Hb), blood glucose (BS), brain natriuretic peptide (BNP), and cardiac function between HP patients (HP group; n=12) and sex- and age-matched control patients (NP group; n=12). There were no significant differences of age, sex, and duration of HD between the 2 groups. Cardiothoracic ratio is bigger and BNP is higher in the HP group compared with the NP group (CTR: HP 55.8+/-2.9% vs. NP 47.7+/-1.1%, p=0.0165; BNP: HP 602+/-171 vs. NP 147+/-38, p=0.0167). Serum albumin in the HP group is significantly lower compared with the NP group (HP 3.2+/-0.1 g/dL vs. NP 3.5+/-0.1 g/dL, p=0.0130). However, there were no significant differences of ultrafiltration rate (UFR), BS, TC, Hb, and cardiac function between the 2 groups. There is a significant negative correlation between changes of systolic blood pressure (delta systolic blood pressure) and serum albumin in these patients (r=-0.598, p=0.0016). From these data, we conclude that hypoalbuminemia is a major risk factor of hypotension during HD.

RELATIONSHIP BETWEEN THE PRESENCE OF HELICOBACTER PYLORI WITH INFLAMMATORY ENDOSCOPIC CHANGES IN GASTRODUODENAL MUCOSA.

Science.gov (United States)

Ribeiro, Irma Cláudia Saboya; Kubrusly, Luiz Fernandao; Nassif, Paulo Afonso Nunes; Ribeiro, Patrícia Fernanda Saboya; Veras, Rodrigo de Oliveira; Neppel, Aline

2016-01-01

The influence of Helicobacter pylori (HP) in inflammatory disorders of the digestive mucosa has been the subject of several studies since socioeconomic, personal and environmental factors were implicated in the bacteria transmission. To correlate the inflammatory endoscopic findings with HP infection and the onset of mucosal diseases mucous of the upper digestive tract. Comparative observational study, in which were collected data from 2247 patients who underwent upper endoscopy and biopsies for HP with urease test. The patients were divided into two groups: HP+ and HP- (control) in which endoscopic findings were observed for the following changes: esophagitis, esophageal ulcer, gastritis, erosive gastritis, gastric ulcer, bulboduodenitis, bulbar ulcer and without disease. As for esophagitis, there was little disparity in the distribution favorable to HP+ group (HP+ =67.11% and HP- =69.89%) and esophageal ulcer (HP+ =0% and HP- =0, 21%). Gastritis was favorable to HP- group (HP+ =78.34% and HP- =73.63%), as well as erosive gastritis (HP+ = 67,11% and HP- = 64,55%), in bulboduodenitis (HP+ =1,87% and HP- 1,23%), in gastric ulcer (HP+ =2,14% and HP- =2,03%) and in the absence of alterations in the HP+ group (4.81%) with the HP- control group (6,30%), in which there was little disproportion in favor of HP- group, but without statistical significance. As for the bulbar ulcer (HP +=10.16% and HP- =4.48%), there was statistically significant (p=0.00001). There is no difference between HP+ and HP- groups in inflammatory changes in endoscopic gastroduodenal mucosa, except for the relationship between HP and bulbar ulcer. A influência do Helicobacter pylori (HP) nas alterações inflamatórias das mucosas digestivas tem sido objeto de vários estudos uma vez que fatores socioeconômicos, pessoais e ambientais são implicados na transmissão da bactéria, facilitando-a. Relacionar os achados inflamatórios endoscópicos com a infecção pelo HP e o aparecimento de doen

Hypoxia-inducible factor-1 alpha has a key role in hypoxic preconditioning.

Science.gov (United States)

Taie, Satoshi; Ono, Junichiro; Iwanaga, Yasuyuki; Tomita, Shuhei; Asaga, Takehiko; Chujo, Kosuke; Ueki, Masaaki

2009-08-01

Sublethal hypoxia induces tolerance to subsequent hypoxic insults in a process known as hypoxic preconditioning (HP). Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is a key transcription protein involved in the mechanism of HP. In this study, we investigated the effects of HP on tissue oxygenation and expression of HIF-1 alpha gene targets in the brain using neural cell-specific HIF-1 alpha-deficient mice. The animals were exposed to 8% oxygen for 3 hours. Twenty-four hours later, the oxygen partial pressure (pO(2)) of brain tissue and gene expression were measured during hypoxia. HP improved the pO(2) of brain tissue during subsequent hypoxia with upregulated inducible nitric oxide synthase in wild-type mice, whereas HP had no detectable effect in the mutant mice. Our results indicate that the protective effects of HP may be partially mediated by improving tissue oxygenation via HIF-1 alpha and inducible nitric oxide synthase.

Catalyst for Change

Science.gov (United States)

Barton, Casey; Collura, Kenneth

2003-01-01

As a pioneer in education technology, Bishop Hartley High School, a Catholic high school in Columbus, Ohio, has sought to embrace change with a revolutionary computer from Hewlett-Packard known as the Compaq Tablet PC TC1000. In February 2003, Bishop Hartley became the first high school in the country to give an entire student class their own…

Effect of a High Protein Weight Loss Diet on Weight, High-Sensitivity C-Reactive Protein, and Cardiovascular Risk among Overweight and Obese Women: A Parallel Clinical Trial

Directory of Open Access Journals (Sweden)

Leila Azadbakht

2013-01-01

Full Text Available Studies regarding the effects of high protein (HP diets on cardiovascular (CVD risk factors have reported contradictory results. We aimed to determine the effects of an HP diet on CVD risk factors and high-sensitivity C-reactive protein (hs-CRP among overweight and obese women. In this randomized controlled trial, we recruited 60 overweight and obese women, aged 20–65, into an HP or energy-restricted control diet for three months (protein, carbohydrate, and fat: 25%, 45%, and 30% versus 15%, 55%, and 30%, resp.. Total protein was divided between animal and plant sources in a 1 : 1 ratio, and animal sources were distributed equally between meats and dairy products. Fasting blood samples, hs-CRP, lipid profile, systolic and diastolic blood pressure, and anthropometric measurements were assessed using standard guidelines. Percent change was significantly different between the two diet groups for weight (standard protein (SP: −3.90 ± 0.26 versus HP: −6.10 ± 0.34%; , resp. and waist circumference (SP: −3.03 ± 0.21 versus HP: −5.06 ± 0.28%; , resp.. Percent change of fasting blood glucose (FBG substantially decreased in the control group compared to the HP group (−9.13 ± 0.67 versus −4.93 ± 1.4%; P = 0.01, resp.. Total cholesterol, systolic blood pressure (SBP, and diastolic blood pressure (DBP decreased both in the HP and in the control diet groups (P = 0.06, P = 0.07, and P = 0.09, resp.; however, the results were marginally significant. Serum levels of hs-CRP were reduced both in the control (−0.08 ± 0.11%, P = 0.06 and in the high protein groups (−0.04 ± 0.09%, P = 0.06. The energy-restricted HP diet resulted in more beneficial effects on weight loss and reduction of waist circumference. CVD risk factors may improve with HP diets among overweight and obese women. When using isoenergetic weight loss diets, total cholesterol, hs-CRP, and SBP were marginally significantly reduced, independent of dietary protein content

Personal dose equivalent conversion coefficients for electrons to 1 Ge V.

Science.gov (United States)

Veinot, K G; Hertel, N E

2012-04-01

In a previous paper, conversion coefficients for the personal dose equivalent, H(p)(d), for photons were reported. This note reports values for electrons calculated using similar techniques. The personal dose equivalent is the quantity used to approximate the protection quantity effective dose when performing personal dosemeter calibrations and in practice the personal dose equivalent is determined using a 30×30×15 cm slab-type phantom. Conversion coefficients to 1 GeV have been calculated for H(p)(10), H(p)(3) and H(p)(0.07) in the recommended slab phantom. Although the conversion coefficients were determined for discrete incident energies, analytical fits of the conversion coefficients over the energy range are provided using a similar formulation as in the photon results previously reported. The conversion coefficients for the personal dose equivalent are compared with the appropriate protection quantity, calculated according to the recommendations of the latest International Commission on Radiological Protection guidance. Effects of eyewear on H(p)(3) are also discussed.

Hydroxypropyl-sulfobutyl-β-cyclodextrin improves the oral bioavailability of edaravone by modulating drug efflux pump of enterocytes.

Science.gov (United States)

Rong, Wen-Ting; Lu, Ya-Peng; Tao, Qing; Guo, Miao; Lu, Yu; Ren, Yong; Yu, Shu-Qin

2014-02-01

The objective of the study was to evaluate the effect of hydroxypropyl-sulfobutyl-β-cyclodextrin (HP-SBE-βCD) on the bioavailability and intestinal absorption of edaravone, and identify its mechanism of action. We devised HP-SBE-βCD as a carrier and modulator of P-glycoprotein (Pgp) efflux pump, and edaravone as a model drug, and prepared edaravone/HP-SBE-βCD inclusion complex. HP-SBE-βCD improved the water solubility and enhanced the bioavailability of edaravone by 10.3-fold in rats. Then, in situ single-pass intestinal perfusion showed that HP-SBE-βCD had an effect of improving the permeability and inhibiting the efflux of edaravone. Furthermore, the effects of HP-SBE-βCD on Pgp were achieved through interfering with the lipid raft and depleting the cholesterol of enterocytes membrane. From the results, we presented the novel mechanisms. First, edaravone/HP-SBE-βCD had a lower release from the inclusion compound to protect edaravone from the low pH of the stomach. Then, HP-SBE-βCD modulated the membrane microenvironment of intestinal absorption epithelial cells. At last, the result was that HP-SBE-βCD enhanced the absorption of edaravone by interfering with Pgp. In conclusion, HP-SBE-βCD improves the bioavailability of drug not only because of its enhancing water solubility of the drug, but also because it modulates the Pgp-mediated efflux from enterocytes. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association.

Performance Evaluation of HP/ORC (Heat Pump/Organic Rankine Cycle) System with Optimal Control of Sensible Thermal Storage

DEFF Research Database (Denmark)

Do Carmo, Carolina Madeira Ramos; Dumont, Olivier; Nielsen, Mads Pagh

2016-01-01

In energy systems with high share of renewable energy sources, like wind and solar power, it is paramount to deal with their intrinsic variability. The interaction between electric and thermal energy (heating and cooling) demands represent a potential area for balancing supply and demand that could...... come to contribute to the integration of intermittent renewables.This paper describes an innovative concept that consists of the addition of an Organic Rankine Cycle (ORC) to a combined solar system coupled to a ground-source heat pump (HP) in a single-family building. The ORC enables the use of solar...... energy in periods of no thermal energy demand and reverses the heat pump cycle to supply electrical power. A dynamic model based on empirical data of this system is used to determine the annual performance. Furthermore, this work assesses the benefits of different control strategies that address...

Effect of the “protein diet” and bone tissue.

Science.gov (United States)

Nascimento da Silva, Zoraide; Azevedo de Jesuz, Vanessa; De Salvo Castro, Eduardo; Soares da Costa, Carlos Alberto; Teles Boaventura, Gilson; Blondet de Azeredo, Vilma

2014-01-01

The aim of this study is to evaluate the effect of the hyperproteic diet consumption on bone tissue. The study was conducted during sixty days. Twenty eight Wistar albinus rats, adults, originated from Laboratory of Experimental Nutrition were divided in four groups: (n = 7); Control 1 (C1), Control 2 (C2), Hyperproteic 1 (HP1) e Hyperproteic 2 (HP2). The C2 and HP2 groups were submitted to 30% of food restriction. The hyperproteic diet was based on the Atkins diet and prepared to simulate the protein diet. At the end of the study the animals were anesthetized to performer bone densitometry analyses by DEXA and blood and tissue collection. Serum and bone minerals analyses were conducted by colorimetric methods in automated equipment. The total bone mineral density (BMD) of the pelvis and the spine of the food restriction groups (HP2 e C2) were lower (p hyperproteic groups (HP1 e HP2). It was observed similar effect on the osteocalcin level, that presented lower (p hyperproteic groups. The insulin level was lower only in HP2 and serum calcium of the HP1 and HP2 groups was lower than C1. The protein diet promotes significant bone change on femur and in the hormones levels related to bone synthesis and maintenance of this tissue.

EASI on the HP-25, HP-65, and HP-67

International Nuclear Information System (INIS)

Sasser, D.W.

1977-05-01

EASI (Estimate of Adversary Sequence Interruption) is an effective, simple method which has been developed for use in evaluating physical security systems. The usefulness of the method is enhanced by the fact that it can be implemented on a programmable pocket calculator. A program for the Texas Instruments SR-52 programmable pocket calculator has been developed and reported upon elsewhere. The purpose of this report is to provide programs for the Hewlett-Packard programmable pocket calculators

Aproximações entre história pública e história oral: o caso do Laboratório de História Oral da Univille

Directory of Open Access Journals (Sweden)

Ilanil Coelho

2016-12-01

Full Text Available Neste artigo, construímos uma aproximação teórico-metodológica entre a História Pública (HP e História Oral (HO. O texto encontra-se estruturado em três partes. Na primeira, além de apresentarmos nossa perspectiva conceitual sobre HP, refletimos sobre a historicidade da noção de HP na Inglaterra, nos Estados Unidos e no Brasil, pontuando seus diversos cruzamentos com a HO. Em seguida, com base numa ampla pesquisa documental, perscrutamos os sentidos de História Oral e o papel atribuído ao historiador e ao conhecimento histórico durante a criação do Laboratório de História Oral da Universidade da Região de Joinville (LHO/Univille. Nesse fazer, analisamos como, no início dos anos 1980, esse espaço foi concebido como parte de uma rede de produção de fontes orais que envolvia numerosas instituições de ensino superior do sul do Brasil e, também, entidades internacionais de atuação multilateral, tais como a Organização dos Estados Americanos (OEA e a Secretaria de Cooperação Técnica Internacional do Brasil, subordinada ao então Ministério de Educação e Cultura (SUBIN/MEC. No terceiro e último item, finalizamos o texto tecendo algumas considerações sobre os limites e as possibilidades reflexivas da HP quando articulada à HO e aos recentes debates sobre o papel público do historiador na escrita da História do Tempo Presente.   Palavras-chave: História Pública; História Oral; História do Tempo Presente; LHO/Univille.

Performance Evaluation of a HP/ORC (Heat Pump/Organic Rankine Cycle) System with Optimal Control of Sensible Thermal Storage

DEFF Research Database (Denmark)

Carmo, Carolina; Nielsen, Mads P.; Elmegaard, Brian

2016-01-01

In energy systems with high share of renewable energy sources, like wind and solar power, it is paramount to deal with their intrinsic variability. The interaction between electric and thermal energy (heating and cooling) demands representa potential area for balancing supply and demand that could...... come to contribute to the integration of intermittent renewables.This paper describes an innovative concept that consists of the addition of an Organic Rankine Cycle (ORC) toa combined solar system coupled to a ground-source heat pump (HP) in a single-family building. The ORC enables the use of solar...... energy in periods of no thermal energy demand and reverses the heat pump cycle to supply electricalpower. A dynamic model based on empirical data of this system is used to determine the annual performance. Furthermore, this work assesses the benefits of different control strategies that address...

Finite Element Modeling of Material Fatigue and Cracking Problems for Steam Power System HP Devices Exposed to Thermal Shocks

Directory of Open Access Journals (Sweden)

Pawlicki Jakub

2016-09-01

Full Text Available The paper presents a detailed analysis of the material damaging process due to low-cycle fatigue and subsequent crack growth under thermal shocks and high pressure. Finite Element Method (FEM model of a high pressure (HP by-pass valve body and a steam turbine rotor shaft (used in a coal power plant is presented. The main damaging factor in both cases is fatigue due to cycles of rapid temperature changes. The crack initiation, occurring at a relatively low number of load cycles, depends on alternating or alternating-incremental changes in plastic strains. The crack propagation is determined by the classic fracture mechanics, based on finite element models and the most dangerous case of brittle fracture. This example shows the adaptation of the structure to work in the ultimate conditions of high pressure, thermal shocks and cracking.

Effects of the dietary amount and source of protein, resistance training and anabolic-androgenic steroids on body weight and lipid profile of rats.

Science.gov (United States)

Aparicio, V A; Sánchez, C; Ortega, F B; Nebot, E; Kapravelou, G; Porres, J M; Aranda, P

2013-01-01

Dietary protein amount and source, hypertrophy resistance training (RT) and anabolicandrogenic steroids (AAS) may affect body weight and plasma and hepatic lipid profile. 157 adult male Wistar rats were randomly distributed in 16 experimental groups resulting in: normal-protein (NP) or high-protein (HP) diets, whey or soy-protein diets, with or without RT and with or without AAS, for 3 months. Final body weight was lower in the RT and AAS groups compared to sedentary and non- AAS groups, respectively (all, pweight of rats that performed RT or ingested a HP diet (all, p<0.05). HDL-cholesterol was higher when RT was combined with HP diets (p=0.010) or non-AAS and when HP diets were combined with non-AAS (both,p<0.001). Groups that combined RT with non-AAS administration obtained the lowest hepatic TAG (p<0.05). Among all the interventions tested, AAS was the factor that most negatively affected plasma and hepatic lipid profile, whereas HP diets and RT could benefit lipid profile, especially when combined. Copyright © AULA MEDICA EDICIONES 2013. Published by AULA MEDICA. All rights reserved.

Unified Hall-Petch description of nano-grain nickel hardness, flow stress and strain rate sensitivity measurements

Science.gov (United States)

Armstrong, R. W.; Balasubramanian, N.

2017-08-01

It is shown that: (i) nano-grain nickel flow stress and hardness data at ambient temperature follow a Hall-Petch (H-P) relation over a wide range of grain size; and (ii) accompanying flow stress and strain rate sensitivity measurements follow an analogous H-P relationship for the reciprocal "activation volume", (1/v*) = (1/A*b) where A* is activation area. Higher temperature flow stress measurements show a greater than expected reduction both in the H-P kɛ and in v*. The results are connected with smaller nano-grain size (tested at very low imposed strain rates.

High pressure 129I Moessbauer studies of GeI4 molecular crystals

International Nuclear Information System (INIS)

Pasternak, M.P.; Los Alamos National Lab.; Taylor, R.D.

1990-01-01

The Moessbauer effect in 129 I in cunjunction with Diamond-Anvil-Cell high pressure techniques was applied to investigate the high pressure phase(s) of the molecular crystal GeI 4 . The 129 I Quadrupole Interaction was the main probe for characterizing the intermolecular structural transformation with pressure. With increasing pressure, at about 15 GPa, the onset of a partial molecular-association phase (HP1) is first observed. In HP1 two out of the four iodines strongly overlap to form linear chains of GeI 4 . The HP1 phase coexists with the low pressure (LP) molecular phase, but its population increases with increasing pressure. At P≅20 GPa a second high pressure phase (HP2) is identified where all four iodines strongly overlap to form a three dimensional, fully molecular-associated structure. With increasing pressure and at P>20 GPa, HP2 is the only phase up to P=34 GPa, the highest pressure used. A significant hysteresis of the relative abundances with pressure is observed. The isomer shift of the HP2 and HP1 structures is considerably larger than that of the LP one. (orig.)

45 CFR 2510.20 - Definitions.

Science.gov (United States)

2010-10-01

... remuneration in an educational institution to assist students of out-of-school youth; and (2) Is beyond the age... expenses for training and travel. (2) Costs (including salary, benefits, training, travel) attributable to... certificate received by a student after the student has passed a State-authorized examination that the State...

Relationships between craniocervical posture and pain-related disability in patients with cervico-craniofacial pain

Science.gov (United States)

López-de-Uralde-Villanueva, Ibai; Beltran-Alacreu, Hector; Paris-Alemany, Alba; Angulo-Díaz-Parreño, Santiago; La Touche, Roy

2015-01-01

Objectives This cross-sectional correlation study explored the relationships between craniocervical posture and pain-related disability in patients with chronic cervico-craniofacial pain (CCFP). Moreover, we investigated the test–retest intrarater reliability of two craniocervical posture measurements: head posture (HP) and the sternomental distance (SMD). Methods Fifty-three asymptomatic subjects and 60 CCFP patients were recruited. One rater measured HP and the SMD using a cervical range of motion device and a digital caliper, respectively. The Spanish versions of the neck disability index and the craniofacial pain and disability inventory were used to assess pain-related disability (neck disability and craniofacial disability, respectively). Results We found no statistically significant correlations between craniocervical posture and pain-related disability variables (HP and neck disability [r=0.105; P>0.05]; HP and craniofacial disability [r=0.132; P>0.05]; SMD and neck disability [r=0.126; P>0.05]; SMD and craniofacial disability [r=0.195; P>0.05]). A moderate positive correlation was observed between HP and SMD for both groups (asymptomatic subjects, r=0.447; CCFP patients, r=0.52). Neck disability was strongly positively correlated with craniofacial disability (r=0.79; Pposture, but these differences were very small (mean difference =1.44 cm for HP; 6.24 mm for SMD). The effect sizes reached by these values were estimated to be small for SMD (d=0.38) and medium for HP (d=0.76). Conclusion The results showed no statistically significant correlations between craniocervical posture and variables of pain-related disability, but a strong correlation between the two variables of disability was found. Our findings suggest that small differences between CCFP patients and asymptomatic subjects exist with respect to the two measurements used to assess craniocervical posture (HP and SMD), and these measures demonstrated high test–retest intrarater reliability for

The effect of breakfast type and frequency of consumption on glycemic response in overweight/obese late adolescent girls.

Science.gov (United States)

Alwattar, A Y; Thyfault, J P; Leidy, H J

2015-08-01

The primary aim was to examine the daily glycemic response to normal-protein (NP) vs higher-protein (HP) breakfasts in overweight adolescents who habitually skip breakfast (H-BS). The secondary aim examined whether the glycemic response to these meals differed in H-BS vs habitual breakfast consumers (H-BC). Thirty-five girls (age: 19 ± 1 year; body mass index: 28.4 ± 0.7 kg/m(2)) participated in the semi-randomized crossover-design study. The participants were grouped according to habitual breakfast frequency. H-BS (n = 20) continued to skip breakfast (BS) or consumed a NP (12 g protein) or HP (32 g protein) breakfast for 3 days, whereas the H-BC (n = 15) completed the NP and HP breakfast conditions for 3 days. On day 4 of each pattern, an 8 h testing day was completed. The respective breakfast and a standard lunch meal were provided, and plasma was collected to assess morning, afternoon, and total glucose and insulin area under the curves (AUC). In H-BS, the addition of a HP breakfast increased total glucose AUC vs BS (P < 0.05), whereas NP breakfast increased total insulin AUC vs BS (P < 0.05). In H-BC, the HP breakfast reduced morning, afternoon and total glucose AUCs vs NP (all, P < 0.05). No differences in insulin were detected. When comparing the HP-NP differential glycemic responses between groups, H-BS experienced greater afternoon and total glucose AUCs following HP vs NP breakfasts (both, P<0.05). No differences in insulin responses were observed between groups. Novel differences in the glucose response to HP vs NP breakfasts were observed and were influenced by the frequency of habitual breakfast consumption in overweight adolescents.

Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway

Directory of Open Access Journals (Sweden)

Sara Cheleschi

2017-01-01

Full Text Available Mechanical loading and hydrostatic pressure (HP regulate chondrocytes’ metabolism; however, how mechanical stimulation acts remain unclear. MicroRNAs (miRNAs play an important role in cartilage homeostasis, mechanotransduction, and in the pathogenesis of osteoarthritis (OA. This study investigated the effects of a cyclic HP (1–5 MPa, in both normal and OA human chondrocytes, on the expression of miR-27a/b, miR-140, miR-146a/b, and miR-365, and of their target genes (MMP-13, ADAMTS-5, IGFBP-5, and HDAC-4. Furthermore, we assessed the possible involvement of Wnt/β-catenin pathway in response to HP. Chondrocytes were exposed to HP for 3h and the evaluations were performed immediately after pressurization, and following 12, 24, and 48 h. Total RNA was extracted and used for real-time PCR. β-catenin was detected by Western blotting analysis and immunofluorescence. In OA chondrocytes, HP induced a significant increase (p < 0.01 of the expression levels of miR-27a/b, miR-140, and miR-146a, and a significant reduction (p < 0.01 of miR-365 at all analyzed time points. MMP-13, ADAMTS-5, and HDAC-4 were significantly downregulated following HP, while no significant modification was found for IGFBP-5. β-catenin levels were significantly increased (p < 0.001 in OA chondrocytes at basal conditions and significantly reduced (p < 0.01 by HP. Pressurization did not cause any significant modification in normal cells. In conclusion, in OA chondrocytes, HP restores the expression levels of some miRNAs, downregulates MMP-13, ADAMTS-5, and HDAC-4, and modulates the Wnt/β-catenin pathway activation.

Stability Estimates for h-p Spectral Element Methods for Elliptic Problems

NARCIS (Netherlands)

Dutt, Pravir; Tomar, S.K.; Kumar, B.V. Rathish

2002-01-01

In a series of papers of which this is the first we study how to solve elliptic problems on polygonal domains using spectral methods on parallel computers. To overcome the singularities that arise in a neighborhood of the corners we use a geometrical mesh. With this mesh we seek a solution which

Application of 13C-urea breath test in patients diagnosed as H. pylori-negative by gastroscopy

International Nuclear Information System (INIS)

Li Long; Liu Changjiang; Gao Jianqing; Xu Sumei; Chen Linna; Zhou Jianming

2006-01-01

13 C-urea breath test( 13 C-UBT)was used to evaluate infection rate of H. pylori(HP) and effect of HP eradication in patients diagnosed as HP-negative by histology and rapid urease test. Patiens without gastrointestinal disorders were set as control group. Within 640 patients diagnosed as HP-negative by histology and rapid urease test, there were 389 patients showed HP-positive by 13 C-UBT. The positive rate of HP was 60.8%. 389 patients diagnosed as HP- positive by 13 C-UBT were treated with PPI-based triple therapy, PPI-based double therapy and single PPI therapy, respectively. After treatment, the negative rate of 13 C-UBT was 83.8%, 18.4% and 3.3%, respectively. The results showed significant difference between three kinds of therapy (P 13 C-UBT could improve the diagnostic rate of HP to patients who diagnosed as HP-negative by histology and rapid urease test. (authors)

Oxidative and ER stress-dependent ASK1 activation in steatotic hepatocytes and Kupffer cells sensitizes mice fatty liver to ischemia/reperfusion injury.

Science.gov (United States)

Imarisio, Chiara; Alchera, Elisa; Bangalore Revanna, Chandrashekar; Valente, Guido; Follenzi, Antonia; Trisolini, Elena; Boldorini, Renzo; Carini, Rita

2017-11-01

Steatosis intensifies hepatic ischemia/reperfusion (I/R) injury increasing hepatocyte damage and hepatic inflammation. This study evaluates if this process is associated to a differential response of steatotic hepatocytes (HP) and Kupffer cells (KC) to I/R injury and investigates the molecular mechanisms involved. Control or steatotic (treated with 50 μmol palmitic acid, PA) mouse HP or KC were exposed to hypoxia/reoxygenation (H/R). C57BL/6 mice fed 9 week with control or High Fat diet underwent to partial hepatic IR. PA increased H/R damage of HP and further activated the ASK1-JNK axis stimulated by ER stress during H/R. PA also induced the production of oxidant species (OS), and OS prevention nullified the capacity of PA to increase H/R damage and ASK1/JNK stimulation. ASK1 inhibition prevented JNK activation and entirely protected HP damage. In KC, PA directly activated ER stress, ASK1 and p38 MAPK and increased H/R damage. However, in contrast to HP, ASK1 inhibition further increased H/R damage by preventing p38 MAPK activation. In mice liver, steatosis induced the expression of activated ASK1 in only KC, whereas I/R exposure of steatotic liver activated ASK1 expression also in HP. "In vivo", ASK1 inhibition prevented ASK1, JNK and p38 MAPK activation and protected I/R damage and expression of inflammatory markers. Lipids-induced ASK1 stimulation differentially affects HP and KC by promoting cytotoxic or protective signals. ASK1 increases H/R damage of HP by stimulating JNK and protects KC activating p38MAPK. These data support the potentiality of the therapeutic employment of ASK1 inhibitors that can antagonize the damaging effects of I/R upon fatty liver surgery by the contextual reduction of HP death and of KC-mediated reactions. Copyright © 2017 Elsevier Inc. All rights reserved.

Virulence Plasmid (pYV-Associated Expression of Phenotypic Virulent Determinants in Pathogenic Yersinia Species: A Convenient Method for Monitoring the Presence of pYV under Culture Conditions and Its Application for Isolation/Detection of Yersinia pestis in Food

Directory of Open Access Journals (Sweden)

Saumya Bhaduri

2011-01-01

Full Text Available In Yersinia pestis, Y. pseudotuberculosis, and Y. enterocolitica, phenotypic expression of virulence plasmid (pYV: 70-kb-associated genetic determinants may include low-calcium response (Lcr, pinpoint colony, size = 0.36 mm, colony morphology (size = 1.13 mm, crystal violet (CV binding (dark-violet colony, Congo Red (CR uptake (red pinpoint colony, size = 0.36 mm, autoagglutination (AA = cells agglutinate, and hydrophobicity (HP = clumping of cells. Y. pseudotuberculosis is chromosomally closely related to Y. pestis; whereas, Y. enterocolitica is chromosomally more distantly related to Y. pestis and Y. pseudotuberculosis. All three species demonstrate Lcr, CV binding, and CR uptake. The colony morphology/size, AA, and HP characteristics are expressed in both Y. pseudotuberculosis and Y. enterocolitica but not in Y. pestis. Congo red uptake in Y. pestis was demonstrated only on calcium-deficient CR magnesium oxalate tryptic soy agar (CR-MOX, whereas this phenotype was expressed on both CR-MOX and low-calcium agarose media in Y. pseudotuberculosis and Y. enterocolitica. These phenotypes were detectable at 37°C within 24 h in Y. enterocolitica and Y. pseudotuberculosis but did not appear until 48 h in Y. pestis due to its slower growth rate at 37°C. The pYV is unstable (i.e., easily lost under a variety of culture conditions in all three species but is more unstable in Y. pestis. The specific CR uptake by Y. pestis in CR-MOX and the delayed time interval to express Lcr and CR uptake provide a means to differentiate Y. pestis from Y. enterocolitica and Y. pseudotuberculosis. These differences in pYV expression in Y. pestis can be used for its isolation and detection in food.

Using computer program RELAP5/MOD2 on microcomputers

International Nuclear Information System (INIS)

Grgic, D.; Bajs, T; Cavlina, N.; Debrecin, N.

1990-01-01

Our work on installation of RELAP5/MOD2 code on IBM4341, mVAX 11, MGT-386 and COMPAQ-386/20e computers is described. Main characteristics of RELAP5/MOD2 structure programming style and differences between FORTRAN VS, VAX-11 FORTRAN and NDP FORTRAN 386 are presented. We discussed basic philosophy used in modification and testing and test results. (author)

STUDY OF SERUM HAPTOGLOBIN LEVEL AND ITS RELATION TO ERYTHROPOIETIC ACTIVITY IN BETA THALASSEMIA CHILDREN .

Directory of Open Access Journals (Sweden)

Seham Ragab

2015-02-01

Full Text Available Background  :Serum haptoglobin (Hp is a reliable marker for hemolysis regardless the inflammatory state.  Objective: We investigated the possible relation between Hp depletion and hemolysis severity, hepatitis C virus (HCV infection and iron load in β-thalassemia children. Methods: Twenty  two β-thalassemia major (TM ,20 β-thalassemia  intermedia (TI children with 20 age and sex matched healthy controls were involved. Pre-transfusion hemoglobin level was considered . Serum ferritin , Hp  and transferrin receptor  levels (sTfR  (by ELISA , alanine aminotransferase (ALT and  aspartate aminotransferase (AST  (by colorimetric method were assayed. Markers of hepatitis C virus  (HCV  were done by PCR. Results:  The mean Hp levels among the studied groups were as follows; 8.02 ± 0.93 (mg/dl , 8.6 ±0.72 (mg/dl  and 122  ± 18.5(mg/dl   for TM ,TI and the controls respectively . Both patient groups had significantly lower Hp level compared to the controls (P<0.0001  with significant lower level in TM compared to TI  children ( P= 0.034  .Significant inverse correlations were  found between serum Hp and sTfR levels in thalassemia children combined and in each group (TM and TI as well as among HCV infected children. STfR   was the only significant independent predictor for  serum Hp level (t= -5.585 , P<0.0001 . Among  HCV infected patients , no significant correlation was found between serum Hp and serum transaminases  .Conclusion:  Serum Hp depletion in thalassemia had significant relation to disease severity and correlated   well with their erythropoietic activity, as assessed by the measurement of  sTfR without significant relation  HCV infection . Large sample  multicenter studies are  recommended.

Texture and structure contribution to low-temperature plasticity enhancement of Mg-Al-Zn-Mn Alloy MA2-1hp after ECAP and annealing

Science.gov (United States)

Serebryany, V. N.; D'yakonov, G. S.; Kopylov, V. I.; Salishchev, G. A.; Dobatkin, S. V.

2013-05-01

Equal channel angular pressing (ECAP) in magnesium alloys due to severe plastic shear deformations provides both grain refinement and the slope of the initial basal texture at 40°-50° to the pressing direction. These changes in microstructure and texture contribute to the improvement of low-temperature plasticity of the alloys. Quantitative texture X-ray diffraction analysis and diffraction of backscattered electrons are used to study the main textural and structural factors responsible for enhanced low-temperature plasticity based on the example of magnesium alloy MA2-1hp of the Mg-Al-Zn-Mn system. The possible mechanisms of deformation that lead to this positive effect are discussed.

A human pericardium biopolymeric scaffold for autologous heart valve tissue engineering: cellular and extracellular matrix structure and biomechanical properties in comparison with a normal aortic heart valve.

Science.gov (United States)

Straka, Frantisek; Schornik, David; Masin, Jaroslav; Filova, Elena; Mirejovsky, Tomas; Burdikova, Zuzana; Svindrych, Zdenek; Chlup, Hynek; Horny, Lukas; Daniel, Matej; Machac, Jiri; Skibová, Jelena; Pirk, Jan; Bacakova, Lucie

2018-04-01

The objective of our study was to compare the cellular and extracellular matrix (ECM) structure and the biomechanical properties of human pericardium (HP) with the normal human aortic heart valve (NAV). HP tissues (from 12 patients) and NAV samples (from 5 patients) were harvested during heart surgery. The main cells in HP were pericardial interstitial cells, which are fibroblast-like cells of mesenchymal origin similar to the valvular interstitial cells in NAV tissue. The ECM of HP had a statistically significantly (p structures of the two tissues, the dense part of fibrous HP (49 ± 2%) and the lamina fibrosa of NAV (47 ± 4%), was similar. In both tissues, the secant elastic modulus (Es) was significantly lower in the transversal direction (p structure and has the biomechanical properties required for a tissue from which an autologous heart valve replacement may be constructed.

Tumor Hypoxia is Independent of Hemoglobin and Prognostic for Loco-regional Tumor Control after Primary Radiotherapy in Advanced Head and Neck Cancer

International Nuclear Information System (INIS)

Nordsmark, Marianne; Overgaard, Jens

2004-01-01

There is evidence that tumor hypoxia adversely affects loco-regional tumor control and survival in head and neck cancer. The aim of the current study was to compare pretreatment tumor oxygenation measured by Eppendorf pO2 electrodes with known prognostic factors in advanced head and neck tumors after definitive radiotherapy, and to evaluate the prognostic significance of these parameters on loco-regional tumor control. Sixty-seven patients, median age 56 years (22-82), all with primary stage III-IV squamous cell carcinoma were available for survival analysis. Tumor oxygenation was described as the fraction of pO2 values=2.5 mmHg (HP2.5) and the median tumor pO2. By regression analysis HP2.5 was independent of known prognostic factors including stage, pretreatment hemoglobin (Hb) and the largest tumor diameter at the site of pO2 measurement. By Kaplan-Meier analysis loco-regional tumor control at 5 years was in favor of less hypoxic tumors using either HP2.5 or median tumor pO2 as descriptors and stratifying by the median values. Also, Hb was prognostic of loco-regional tumor control at 5 years using the median value as cut off. HP2.5 as continuous parameter was highly significant for loco-regional tumor control in a multivariate analysis. In conclusion both HP2.5 and total Hb were prognostic for loco-regional tumor control, but HP2.5 as continuous variable was independently the strongest prognostic indicator for loco-regional tumor control after definitive primary radiotherapy in advanced head and neck tumors

[The Additional Role of Symptom-Reflux Association Analysis of Diagnosis of Gastroesophageal Reflux Disease Using Bravo Capsule pH Test].

Science.gov (United States)

Jung, Kyoungwon; Park, Moo In; Park, Seun Ja; Moon, Won; Kim, Sung Eun; Kim, Jae Hyun

2017-10-25

Since the development of ambulatory esophageal pH monitoring test to diagnose gastroesophageal reflux disease (GERD), several parameters have been introduced. The aim of this study was to assess whether using the symptom index (SI), symptom sensitivity index (SSI), and symptom association probability (SAP), in addition to the DeMeester score (DS), would be useful for interpreting the Bravo pH monitoring test. A retrospective study, which included 68 patients with reflux symptoms refractory to proton pump inhibitor (PPI) therapy who underwent a Bravo capsule pH test between October 2006 and May 2015, was carried out. Acid reflux parameters and symptom reflux association parameters were analyzed. The median percent time of total pHvariation in percent time of total pHpH test, diagnosis of GERD, including reflux hypersensitivity, can be improved by performing an analysis of the symptom-reflux association and of the day-to-day variation.

Hypericum perforatum: pharmacokinetic, mechanism of action, tolerability, and clinical drug-drug interactions.

Science.gov (United States)

Russo, Emilio; Scicchitano, Francesca; Whalley, Benjamin J; Mazzitello, Carmela; Ciriaco, Miriam; Esposito, Stefania; Patanè, Marinella; Upton, Roy; Pugliese, Michela; Chimirri, Serafina; Mammì, Maria; Palleria, Caterina; De Sarro, Giovambattista

2014-05-01

Hypericum perforatum (HP) belongs to the Hypericaceae family and is one of the oldest used and most extensively investigated medicinal herbs. The medicinal form comprises the leaves and flowering tops of which the primary ingredients of interest are naphthodianthrones, xanthones, flavonoids, phloroglucinols (e.g. hyperforin), and hypericin. Although several constituents elicit pharmacological effects that are consistent with HP's antidepressant activity, no single mechanism of action underlying these effects has thus far been found. Various clinical trials have shown that HP has a comparable antidepressant efficacy as some currently used antidepressant drugs in the treatment of mild/moderate depression. Interestingly, low-hyperforin-content preparations are effective in the treatment of depression. Moreover, HP is also used to treat certain forms of anxiety. However, HP can induce various cytochrome P450s isozymes and/or P-glycoprotein, of which many drugs are substrates and which are the main origin of HP-drug interactions. Here, we analyse the existing evidence describing the clinical consequence of HP-drug interactions. Although some of the reported interactions are based on findings from in vitro studies, the clinical importance of which remain to be demonstrated, others are based on case reports where causality can, in some cases, be determined to reveal clinically significant interactions that suggest caution, consideration, and disclosure of potential interactions prior to informed use of HP. Copyright © 2013 John Wiley & Sons, Ltd.

The iron waste from thermal power plant (t.p.p.) as the source of radioactive contamination

International Nuclear Information System (INIS)

Kovacevic, M.; Todorovic, D.; Radenkovic, M.; Joksic, J.; Vukcevic, M.

2006-01-01

The radioactivity control of the iron waste (tubes) raised from the regular maintenance of the Gacko thermo power plant has shown the allocated spots of relatively high surface contamination, mainly beta, and no enhanced levels of absorbed radiation dose in the air. Further laboratory examination of the contaminated tube samples (10- 15 cm length) determined the level and source of contamination. Gamma spectrometry (H.P. Ge, efficiency 23%) has shown the presence of 210 Pb isotope, beta-emitting radionuclide with 47 keV X-ray emission, the member of the 238 U series. The surface contamination and concentration values, determined after samples dissolution in mineral acids, are 2.4 Bq/cm 2 and 0.6 Bq/g, respectively. The radioactivity of the tubes originate from the naturally occurring radionuclides in coal, with 210 Pb activities mostly within 0.01-0.05 Bq/g. During the T.P.P. work, radioactivity is deposed with ash, flying ash and slag and partly released into atmosphere. But, the part of radioactivity is deposited at the tubes and cauldron with gases emanated during the coal combustion. In this way, the iron becomes contaminated by the natural radionuclide during the technological process. Since there are no legislative stipulations about such cases, we have applied the international recommendations saying that dose limit should not be higher than 10 Micro Sv/year for individuals. On this basis T.E.C.D.O.C. 855 define different use of metals contaminated with natural or man-made radionuclides. According this document the contaminated iron with activity lower than 0.7 Bq/g may be further processed in the steel works that is the case with the iron waste produced in the T.P.P. Gacko. (authors)

Kunstnikuks pühitsemine Hype Gallery's / Margit Tõnson

Index Scriptorium Estoniae

Tõnson, Margit, 1978-

2004-01-01

Londonis Old Truman Brewery galeriihoones Hewlett Packardi toetusel kunstiprojekt kuni 28. veebrarini 2004, mille raames loomingulised inimesed saavad end määratleda kunstnikuna. Ainus limiteeriv kriteerium on tähtede HP olemasolu pealkirjas. Kübergaleriisse saadetud töö prinditakse ja riputatakse vana õlletehase seintele

Difference between individuals with left and right hemiparesis in the effect of gluteus medius vibration on body weight shifting.

Science.gov (United States)

Bonan, I; Butet, S; Jamal, K; Yelnik, A; Tasseel Ponche, S; Leplaideur, S

2017-12-01

To test the effect of gluteus medius (GM) vibration on the reduction of asymmetric body weight distribution in individuals with hemiparesis (HP) at two stages of postural recovery. The effects of GM vibration according to the shift of the body weight (%Shift) onto the paretic leg during GM vibration were registered while standing on a force-platform in 40 HP (19 left and 21 right; mean age 54.7±10.6years, mean time after stroke 2.0±1.3months), as soon as they could stand without assistance and 4 to 6 weeks later, and in 40 control subjects (mean age 54.7±10.5years). Without vibration, baseline body weight (BW) distribution was characterized by underloading of the paretic limb (mean BW on the paretic limb 37.2%±13.1%). At the early stage of balance recovery, % shift toward the paretic limb induced by GM vibration differed significantly between left and right HP (P=0.049) and between left HP and controls (C) (P=0.022) and was related to BW asymmetry (r=0.437, P=0.004). Later, GM vibration reduced asymmetric BW distribution in most HP and no difference was found between left and right HP and between left and C. At an advanced stage of postural recovery, GM vibration could help encourage HP to put weight on the affected limb. Interestingly, a behavioral difference was initially observed between right and left HP that could probably be explained by a different strategy due to the baseline severity of the BW asymmetry. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

High-protein diets and renal status in rats

OpenAIRE

Aparicio, V. A.; Nebot, E.; García-del Moral, R.; Machado-Vílchez, M.; Porres, J. M.; Sánchez, C.; Aranda, P.

2013-01-01

Introduction: High-protein (HP) diets might affect renal status. We aimed to examine the effects of a HP diet on plasma, urinary and morphological renal parameters in rats. Material and methods: Twenty Wistar rats were randomly distributed in 2 experimental groups with HP or normal-protein (NP) diets over 12 weeks. Results and discussion: Final body weight was a 10% lower in the HP group (p < 0.05) whereas we have not observed differences on food intake, carcass weight and muscle ashes conten...

Hartmann's Procedure or Primary Anastomosis for Generalized Peritonitis due to Perforated Diverticulitis: A Prospective Multicenter Randomized Trial (DIVERTI).

Science.gov (United States)

Bridoux, Valerie; Regimbeau, Jean Marc; Ouaissi, Mehdi; Mathonnet, Muriel; Mauvais, Francois; Houivet, Estelle; Schwarz, Lilian; Mege, Diane; Sielezneff, Igor; Sabbagh, Charles; Tuech, Jean-Jacques

2017-12-01

About 25% of patients with acute diverticulitis require emergency intervention. Currently, most patients with diverticular peritonitis undergo a Hartmann's procedure. Our objective was to assess whether primary anastomosis (PA) with a diverting stoma results in lower mortality rates than Hartmann's procedure (HP) in patients with diverticular peritonitis. We conducted a multicenter randomized controlled trial conducted between June 2008 and May 2012: the DIVERTI (Primary vs Secondary Anastomosis for Hinchey Stage III-IV Diverticulitis) trial. Follow-up duration was up to 18 months. A random sample of 102 eligible participants with purulent or fecal diverticular peritonitis from tertiary care referral centers and associated centers in France were equally randomized to either a PA arm or to an HP arm. Data were analyzed on an intention-to-treat basis. The primary end point was mortality rate at 18 months. Secondary outcomes were postoperative complications, operative time, length of hospital stay, rate of definitive stoma, and morbidity. All 102 patients enrolled were comparable for age (p = 0.4453), sex (p = 0.2347), Hinchey stage III vs IV (p = 0.2347), and Mannheim Peritonitis Index (p = 0.0606). Overall mortality did not differ significantly between HP (7.7%) and PA (4%) (p = 0.4233). Morbidity for both resection and stoma reversal operations were comparable (39% in the HP arm vs 44% in the PA arm; p = 0.4233). At 18 months, 96% of PA patients and 65% of HP patients had a stoma reversal (p = 0.0001). Although mortality was similar in both arms, the rate of stoma reversal was significantly higher in the PA arm. This trial provides additional evidence in favor of PA with diverting ileostomy over HP in patients with diverticular peritonitis. ClinicalTrials.gov Identifier: NCT 00692393. Copyright © 2017. Published by Elsevier Inc.

Helicobacter pylori cagA+ Is Associated with Milder Duodenal Histological Changes in Chilean Celiac Patients

Directory of Open Access Journals (Sweden)

Yalda Lucero

2017-08-01

A+ strains were more common in infected potential-CD than in active-CD (10/11 vs. 4/10; p = 0.020 and non-CD (10/20; p = 0.025. Among active-CD patients, Foxp3 positivity was significantly higher in subjects with cagA+ Hp+ compared to cagA- Hp+ (p < 0.01 and Hp- (p < 0.01. In cagA+ Hp+ individuals, Foxp3 positivity was also higher comparing active- to potential-CD (p < 0.01. TGF-β expression in duodenum was similar in active-CD with cagA+ Hp+ compared to Hp- and was significantly downregulated in cagA+ potential-CD subjects compared to other groups.Conclusion: Hp infection rates were similar among individuals with/without CD, but infection with cagA+ strains was associated with milder histological damage in celiac patients infected by Hp, and in active-CD cases with higher expression of T-reg markers. Results suggest that infection by cagA+ Hp may be protective for CD progression, or conversely, that these strains are prone to colonize intestinal mucosa with less severe damage.

Study of charge symmetry in 4He by simultaneous 4He(e,e'p)3H and 4He(e,e'n)3He measurements

International Nuclear Information System (INIS)

Spahn, M.; Kihm, T.; Knopfle, K.T.; Friedrich, J.; Voegler, N.; Schmitt, C.; Walther, V.H.; Unkelbach, M.; Hofmann, H.M.

1989-01-01

We have measured the ( 3 H+p) and ( 3 He+n) breakup of 4 He by 4 He(e,e'c) coincidence experiments at low momentum transfer (q∼0.39 fm -1 ) in the excitation energy region 22≤E x ≤36 MeV. Both channels were studied simultaneously by detecting the charged fragments c=p, 3 H, and 3 He in the same detector system. The measured 4 He(e,e'p) 3 H and 4 He(e,e'n) 3 He angular correlations as well as the deduced total 4 He(e,e'p) 3 H and 4 He(e,e'n) 3 He cross sections are reproduced by microscopic model calculations with a charge-symmetric nuclear Hamiltonian

Point mutation in activated c-Ha-ras gene of a chemically induced transplantable human pancreas carcinoma

International Nuclear Information System (INIS)

Maheshwari, K.K.; Parsa, I.

1986-01-01

The authors have reported a model of human pancreas carcinogenesis where repeated treatment with MNU of explants results in the development of transplantable carcinoma. This report compares the endonuclease digests of DNAs from normal human pancreas (HP) and MNU-induced transplantable tumor (HP-T1) analyzed with 32 P-labelled Ha-ras probe prepared from clone BS-9. The hybridization patterns of BamHI, BglII, EcoRI and HindIII digests of HP were significantly different from those of HP-T1. In EcoRI digests a 3.0 kb fragments of HP-T1 DNA hybridized with Ha-ras probe instead of a 4.3 kb fragments seen in HP DNA. The pattern for HindIII digests was similar to those of EcoRI. The BgIII digests of HP DNA revealed two hybridizing fragments of 8.0 and 4.3 kb whereas those of HP-T1 DNA fragments measured 8.5 and 4.0 kb. BamHI treated HP DNA showed only hybridizing fragments of 6.6 kb while the HP-T1 DNA showed to hybridizing fragments of 6.8 and 7.2 kb. The digested DNAs by HhaI, HinfI, KpnI, pstI, PvuII, SaII, SstI, TaqI and XbaI showed similar hybridization profiles. The point mutation in c-Ha-ras was examined in the HpaII and MspI double digests of both DNAs by 0.6 Kb SmaI fragments of pEJ. The hybridized fragments measured 412 and 355 bp in DNA digests from tumor and normal pancreas respectively

Heading towards the nZEB through CHP+HP systems. A comparison between retrofit solutions able to increase the energy performance for the heating and domestic hot water production in residential buildings

International Nuclear Information System (INIS)

Salata, Ferdinando; Golasi, Iacopo; Domestico, Umberto; Banditelli, Matteo; Lo Basso, Gianluigi; Nastasi, Benedetto; Lieto Vollaro, Andrea de

2017-01-01

Highlights: • Energy optimization measures to increase the energy class of buildings. • Analysis of the demands related to the space-heating season and the production of annual DHW. • Case study related to a residential building of medium size located in Rome (Italy). • Improvements on building envelope and on systems (traditional technologies or CHP+HP). • Energy and economic analysis to achieve the performance of a nZEB. - Abstract: Optimizing consumptions in the field of civil construction led to define energy labels for residential buildings. To calculate the building energy demand the EPgl was determined, i.e. the annual consumption per m"2 of primary energy. This paper examines the technical solutions useful to optimize the energy demands for heating during space-heating season and domestic hot water production (thanks to energy analysis softwares as MC11300 and TRNSYS) and, at the same time, to take into account the financial issues those interventions implied. The total inside heated surface of the building case study is 1204.00 m"2, hence the inside heated volume is about 3250.80 m"3. Besides the more traditional interventions concerning the building envelope and its systems, the paper examined the performance of a system obtained through the combination of a cogenerator (CHP) and a heat pump (HP), thus, substituting the conventional boilers of the buildings. CHP+HP solution increases the most the energy label of the building (from a D class with EPgl = 59.62 kW h m"−"2 year"−"1, to an A class, with EPgl = 25.64 kW h m"−"2 year"−"1), determining an annual energy cost saving of 3,114 € year"−"1, allowing to amortize installation costs (54,560 €) in a reasonable payback period, i.e. 15.4 years. This innovative solution in the residential sector can be realized through retrofit interventions on existing buildings, hence it leads the current dwelling towards nZEB with a remarkable benefits for the environment.

Reliability of eye lens dosimetry in workers of a positron emission tomography radiopharmaceutical production facility

International Nuclear Information System (INIS)

Silva, Teógenes A. da; Guimarães, Margarete C.; Meireles, Leonardo S.; Teles, Luciana L.D.; Lacerda, Marco Aurélio S.

2016-01-01

A new regulatory statement was issued concerning the eye lens radiation protection of persons in planned exposures. A debate was raised on the adequacy of the dosimetric quantity and on its method of measurement. The aim of this work was to establish the individual monitoring procedure with the EYE-D™ holder and a MCP-N LiF:Mg,Cu,P thermoluminescent chip detector for measuring the personal dose equivalent H_p(3) in workers of a Positron Emission Tomography Radiopharmaceutical Production Facility. - Highlights: • New regulatory statement was issued concerning eye lens radiation protection. • The calibration procedure of dosimeters for measuring H_p(3) was studied on a slab and cylindrical phantoms. • H_p(3) measurements in workers in a radiopharmaceutical production facility were done.

Reactive radical-driven bacterial inactivation by hydrogen-peroxide-enhanced plasma-activated-water

Science.gov (United States)

Wu, Songjie; Zhang, Qian; Ma, Ruonan; Yu, Shuang; Wang, Kaile; Zhang, Jue; Fang, Jing

2017-08-01

The combined effects of plasma activated water (PAW) and hydrogen peroxide (H2O2), PAW/HP, in sterilization were investigated in this study. To assess the synergistic effects of PAW/HP, S. aureus was selected as the test microorganism to determine the inactivation efficacy. Also, the DNA/RNA and proteins released by the bacterial suspensions under different conditions were examined to confirm membrane integrity. Additionally, the intracellular pH (pHi) of S. aureus was measured in our study. Electron spin resonance spectroscopy (ESR) was employed to identify the presence of radicals. Finally, the oxidation reduction potential (ORP), conductivity and pH were measured. Our results revealed that the inactivation efficacy of PAW/HP is much greater than that of PAW, while increased H2O2 concentration result in higher inactivation potential. More importantly, as compared with PAW, the much stronger intensity ESR signals and higher ORP in PAW/HP suggests that the inactivation mechanism of the synergistic effects of PAW/HP: more reactive oxygen species (ROS) and reactive nitrogen species (RNS), especially OH and NO radicals, are generated in PAW combined with H2O2 resulting in more deaths of the bacteria.

In growing pigs, the true ileal and total tract digestibility of acid hydrolyzed ether extract in extracted corn oil is greater than in intact sources of corn oil or soybean oil.

Science.gov (United States)

Kim, B G; Kil, D Y; Stein, H H

2013-02-01

An experiment was conducted to determine the true ileal digestibility (TID) and the true total tract digestibility (TTTD) of acid-hydrolyzed ether extract (AEE) in extracted corn oil, high-oil corn, distillers dried grains with solubles (DDGS), corn germ, and high protein distillers dried grains (HP DDG) and to compare these values to the TID and TTTD of AEE in full-fat soybeans. Nineteen barrows with an initial BW of 52.2 kg (SD = 3.8) were fitted with a T-cannula in the distal ileum and allotted to a 19 × 11 Youden square design with 19 diets and 11 periods. A basal diet based on cornstarch, casein, sucrose, and corn bran was formulated. Eighteen additional diets were formulated by adding 3 levels of extracted corn oil, high-oil corn, DDGS, corn germ, HP DDG, or full-fat soybeans to the basal diet. The apparent ileal and the apparent total tract digestibility of AEE were calculated for each diet. The endogenous flow of AEE associated with each ingredient and values for TID and TTTD were calculated using the regression procedure. Results indicated that digested AEE in ileal digesta and feces linearly increased as AEE intake increased regardless of ingredient (P 0.77). However, the ileal and fecal endogenous losses of AEE were different (P oil, HP DDG, and full-fat soybeans. The TID of AEE was greater (P oil (95.4%) than for all other ingredients. The TID of AEE in HP DDG (76.5%) was not different from the TID of AEE in full-fat soybeans (85.2%) but greater (P oil corn, DDGS, and corn germ (53.0, 62.1, and 50.1%, respectively). The TTTD of AEE was greater (P oil (94.3%) than for all other ingredients, and the TTTD in full-fat soybeans (79.7%) was greater (P oil corn, DDGS, corn germ, and HP DDG (41.4, 51.9, 43.9, and 70.2%, respectively). The TTTD of AEE in HP DDG was also greater (P oil corn, DDGS, and corn germ. In conclusion, the intact sources of oil originating from high-oil corn, DDGS, corn germ, or HP DDG are much less digestible than extracted corn oil

Cluster model study of the excited states of /sup 4/He

Energy Technology Data Exchange (ETDEWEB)

Furutani, H. [Osaka Univ., Suita (Japan). Research Center for Nuclear Physics; Ikegami, H.; Muraoka, M. [eds.; Osaka Univ., Suita (Japan). Research Center for Nuclear Physics

1980-01-01

Excited states of /sup 4/He are studied in the energy region E sub(x) = 20 -- 35 MeV within the framework of a (3N + N)-cluster model. (/sup 3/H + p) - (/sup 3/He + n) coupled channel calculation is carried out and results are compared with /sup 3/H(p, p)/sup 3/H, /sup 3/He(n, n)/sup 3/He and /sup 3/H(p, n)/sup 3/He reactions.

Cold snare polypectomy reduced delayed postpolypectomy bleeding compared with conventional hot polypectomy: a propensity score-matching analysis

Science.gov (United States)

Yamashina, Takeshi; Fukuhara, Manabu; Maruo, Takanori; Tanke, Gensho; Marui, Saiko; Sada, Ryota; Taki, Mio; Ohara, Yoshiaki; Sakamoto, Azusa; Henmi, Shinichiro; Sawai, Yugo; Saito, Sumio; Nishijima, Norihiro; Nasu, Akihiro; Komekado, Hideyuki; Sekikawa, Akira; Asada, Masanori; Tumura, Takehiko; Kita, Ryuichi; Kimura, Toru; Osaki, Yukio

2017-01-01

Background and study aims  Cold snare polypectomy (CSP) for small colorectal polyps has lower incidence of adverse events, especially delayed postpolypectomy bleeding (DPPB). However, few data are available on comparisons of the incidence of DPPB of CSP and hot polypectomy (HP). The aim of this study was to evaluate the incidence of DPPB after CSP and compare it with that of HP. A propensity score model was used as a secondary analysis. Patients and methods  This was a retrospective cohort study conducted in a single municipal hospital. We identified 539 patients with colorectal polyps from 2 mm to 11 mm in size who underwent CSP (804 polyps in 330 patients) or HP (530 polyps in 209 patients) between July 2013 and June 2015. Results  There were no cases of DPPB in the CSP group. Conversely, DPPB occurred in 4 patients (1.9 %) after HP, resulting in a significant difference between the CSP and HP groups (0.008 % vs 0 %, P  = 0.02). Propensity score-matching analysis created 402 matched pairs, yielding a significantly higher DPPB rate in the HP group than CSP group (0.02 % vs 0 %, P  = 0.04). However, significantly more patients in the CSP group had unclear horizontal margins that precluded assessment (83 vs 38 cases, P  < 0.001). The retrieval failure rate was significantly higher in the CSP group than in the HP group (3 % vs 0.7 %, P  = 0.01). Conclusions  DPPB was less frequent with CSP than HP, as selected by the propensity score-matching model. Our findings indicate that CSP is recommended polypectomy in daily clinical setting. However, special care should be taken during polyp retrieval and horizontal margin assessment, and these issues could be taken into account in follow-up after CSP. PMID:28670615

Stimulation of growth of the human gastric pathogen Helicobacter pylori by atmospheric level of oxygen under high carbon dioxide tension

Directory of Open Access Journals (Sweden)

Lee Na

2011-05-01

Full Text Available Abstract Background Helicobacter pylori (Hp, a human pathogen that is associated with gastritis, peptic ulcer, and gastric cancer, has been considered a microaerophile, but there is no general consensus about its specific O2 requirements. A clear understanding of Hp physiology is needed to elucidate the pathogenic mechanism(s of Hp infection. Results We cultured Hp under a range of O2 levels with or without 10% CO2 and evaluated growth profiles, morphology, intracellular pH, and energy metabolism. We found that, in the presence of 10% CO2, the normal atmospheric level of O2 inhibited Hp growth at low density but stimulated growth at a higher density. Field emission scanning electron microscopy and fluorescence microscopy of Hp cells cultured under 20% O2 tension revealed live spiral-shaped bacteria with outer membrane vesicles on a rugged cell surface, which became smooth during the stationary phase. Fermentation products including acetate, lactate, and succinate were detected in cell culture media grown under microaerobic conditions, but not under the aerobic condition. CO2 deprivation for less than 24 h did not markedly change cytoplasmic or periplasmic pH, suggesting that cellular pH homeostasis alone cannot account for the capnophilic nature of Hp. Further, CO2 deprivation significantly increased intracellular levels of ppGpp and ATP but significantly decreased cellular mRNA levels, suggesting induction of the stringent response. Conclusions We conclude, unlike previous reports, that H. pylori may be a capnophilic aerobe whose growth is promoted by atmospheric oxygen levels in the presence of 10% CO2. Our data also suggest that buffering of intracellular pH alone cannot account for the CO2 requirement of H. pylori and that CO2 deprivation initiates the stringent response in H. pylori. Our findings may provide new insight into the physiology of this fastidious human pathogen.

[Efficiency of hemoperfusion on clearing thallium based on atomic absorption spectrometry].

Science.gov (United States)

Tian, Tian; Wang, Yongan; Nie, Zhiyong; Wang, Jiao; Peng, Xiaobo; Yuan, Ye; Li, Wanhua; Qiu, Zewu; Xue, Yanping; Xiong, Yiru

2015-04-01

To determine thallium in whole blood by atomic absorption detection method, and to investigate the eliminating effect of hemoperfusion (HP) for thallium in blood. The blood of Beagle dogs which had not exposed to thallium before were obtained for preparation of thallium nitrate ( TlNO3 )-containing solution in three concentrations according to the conversion formula based on animal weight and volume of blood. HP was performed in the simulated in vivo environment. The content of TlNO3 in blood of the next group was determined on the amount of TlNO3 for the last HP of the former dose group. Thallium quantity in different samples was measured with atomic absorption spectrometer blood samples before and after HP. Finally, the thallium concentration in blood was analyzed statistically. Thallium concentrations showed a good linear relationship in the range of 0-200 μg/L (r = 0.998 4). The intra-day precision (RSD) was lower than 4.913%, the intra-day recovery rate was 96.2%-111.9%; the inter-day precision (RSD) was lower than 7.502%, the inter-day recovery rate was 89.6%-105.2%. The concentration of thallium in blood was significantly reduced after HP per time in high, middle, and low dose groups [(453.43 ± 27.80) mg/L to (56.09 ± 14.44) mg/L in high dose group, F = 8.820, P = 0.003; (64.51 ± 13.60) mg/L to (3.19 ± 0.23) mg/L in middle dose group, F = 36.312, P = 0.000; (5.40 ± 0.98) mg/L to (0.38 ± 0.25) mg/L in low dose group, F = 46.240, P = 0.000 ]. The adsorption rate of four times of HP in high, middle and low dose group were (87.63 ± 2.48 )%, (95.06 ± 1.54 )% and (92.76 ± 4.87)%, respectively, without significant difference (F = 4.231, P = 0.070). The method for measuring thallium was established, and it shows a very stable, simple, sensitive for determination of thallium. HP can effectively remove thallium from blood. Thallium concentration can be reduced by 90% after four times of HP. HP is also effective even when thallium concentration is not high.

Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus.

Science.gov (United States)

Lian, Hong; Zeldes, Benjamin M; Lipscomb, Gina L; Hawkins, Aaron B; Han, Yejun; Loder, Andrew J; Nishiyama, Declan; Adams, Michael W W; Kelly, Robert M

2016-12-01

Acetyl-Coenzyme A carboxylase (ACC), malonyl-CoA reductase (MCR), and malonic semialdehyde reductase (MRS) convert HCO 3 - and acetyl-CoA into 3-hydroxypropionate (3HP) in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation cycle resident in the extremely thermoacidophilic archaeon Metallosphaera sedula. These three enzymes, when introduced into the hyperthermophilic archaeon Pyrococcus furiosus, enable production of 3HP from maltose and CO 2 . Sub-optimal function of ACC was hypothesized to be limiting for production of 3HP, so accessory enzymes carbonic anhydrase (CA) and biotin protein ligase (BPL) from M. sedula were produced recombinantly in Escherichia coli to assess their function. P. furiosus lacks a native, functional CA, while the M. sedula CA (Msed_0390) has a specific activity comparable to other microbial versions of this enzyme. M. sedula BPL (Msed_2010) was shown to biotinylate the β-subunit (biotin carboxyl carrier protein) of the ACC in vitro. Since the native BPLs in E. coli and P. furiosus may not adequately biotinylate the M. sedula ACC, the carboxylase was produced in P. furiosus by co-expression with the M. sedula BPL. The baseline production strain, containing only the ACC, MCR, and MSR, grown in a CO 2 -sparged bioreactor reached titers of approximately 40 mg/L 3HP. Strains in which either the CA or BPL accessory enzyme from M. sedula was added to the pathway resulted in improved titers, 120 or 370 mg/L, respectively. The addition of both M. sedula CA and BPL, however, yielded intermediate titers of 3HP (240 mg/L), indicating that the effects of CA and BPL on the engineered 3HP pathway were not additive, possible reasons for which are discussed. While further efforts to improve 3HP production by regulating gene dosage, improving carbon flux and optimizing bioreactor operation are needed, these results illustrate the ancillary benefits of accessory enzymes for incorporating CO 2 into 3HP production in metabolically engineered P

Reducing operator radiation exposure during cardiac resynchronization therapy.

Science.gov (United States)

Brambilla, Marco; Occhetta, Eraldo; Ronconi, Martina; Plebani, Laura; Carriero, Alessandro; Marino, Paolo

2010-12-01

To quantify the reduction in equivalent dose at operator's hand that can be achieved by placement of a radiation-absorbing drape (RADPAD) during long-lasting cardiac resynchronization therapy (CRT) procedures. This is a prospective observational study that included 22 consecutive patients with drug-refractory heart failure who underwent implantation of a CRT device. The cases were randomly assigned to Group A (11 cases), performed without RADPAD, and to Group B (11 cases), performed using RADPAD. Dose equivalent at the examiner's hand was measured as H(p)(0.07) and as a time-adjusted H(p)(0.07) rate (mGy/min) with a direct reading dosimeter. The mean fluoroscopy time was 20.8 ± 7.7 min and the mean dose area product (DAP) was 118.6 ± 45.3 Gy cm(2). No significant differences were found between body mass index, fluoroscopy time, and DAP between patients examined with or without RADPAD. The correlation between the fluoroscopy time and the DAP was high (R(2) = 0.94, P RADPAD at the finger and hand were H(p)(0.07) = 1.27 ± 0.47 mGy per procedure and H(p)(0.07) rate = 0.057 ± 0.011 mGy/min, respectively. The dosage was reduced with the RADPAD to H(p)(0.07) = 0.48 ± 0.20 (P RADPAD. The use of the RADPAD in CRT devices implantation will make unlikely the necessity of limiting the yearly number of implants for high volume operators.

Tensile and electrical properties of unirradiated and irradiated Hycon 3HP{trademark} CuNiBe

Energy Technology Data Exchange (ETDEWEB)

Zinkle, S.J.; Eatherly, W.S. [Oak Ridge National Lab., TN (United States)

1996-10-01

The unirradiated tensile properties of two different heats of Hycon 3HP{trademark} CuNiBe (HT Temper) have been measured over the temperature range of 20-500{degrees}C for longitudinal and long transverse orientations. The room temperature electrical conductivity has also been measured for both heats. Both heats exhibited a very good combination of strength and conductivity at room temperature. The strength remained relatively high at all test temperatures, with a yield strength of 420-520 MPa at 500{degrees}C. However, low levels of ductility (<5% uniform elongation) were observed at test temperatures above 200-250{degrees}C, due to flow localization adjacent to grain boundaries. Fission neutron irradiation to a dose of {approximately}0.7 dpa at temperatures between 100 and 240{degrees}C produced a slight increase in strength and a significant decrease in ductility. The measured tensile elongation increased with increasing irradiation temperature, with a uniform elongation of {approximately}3.3% observed at 240{degrees}C. The electrical conductivity decreased slightly following irradiation, due to the presence of defect clusters and Ni, Zn, Co transmutation products. The data indicate that CuNiBe alloys have irradiated tensile and electrical properties comparable or superior to CuCrZr and oxide dispersion strengthened copper at temperatures <250{degrees}C, and may be suitable for certain fusion energy structural applications.

Meta-analysis of the association of the haptoglobin genotype with cardiovascular outcomes and the pharmacogenomic interactions with vitamin E supplementation

Directory of Open Access Journals (Sweden)

Asleh R

2018-04-01

Full Text Available Rabea Asleh,1,2 Alexandros Briasoulis,3 Elliot M Berinstein,1 Joshua B Wiener,1 Mohan Palla,4 Sudhir S Kushwaha,2 Andrew P Levy1 1Bruce and Rappaport Faculty of Medicine, Technion Institute of Technology, Haifa, Israel; 2Department of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA; 3Division of Cardiovascular Diseases, University of Iowa Hospitals and Clinics, Iowa City, IA, USA; 4Department of Cardiology, Detroit Medical Center, Wayne State University, Detroit, MI, USA Objectives: The objectives of the study were to compile and summarize the data from all of the clinical trials designed to examine the association between haptoglobin (Hp genotype and incidence of cardiovascular (CV events in patients with diabetes mellitus (DM and to assess the impact of vitamin E treatment on CV outcomes according to the Hp genotype.Background: The Hp genotype could serve as a predictive biomarker to DM patients who may benefit from vitamin E therapy.Methods: The electronic databases MEDLINE, PubMed, EMBASE and the Cochrane Library for Central Register of Clinical Trials were searched systematically using the following MESH terms: “haptoglobin genotype”, “diabetes mellitus” and “cardiovascular events”.Results: Overall, 13 studies fit the inclusion criteria for this analysis, yielding a large study population that included 6,161 patients without Hp 2-2 and 4,684 patients with Hp 2-2. The analysis of these studies showed that the incidence of CV events in DM patients with the Hp 2-2 genotype was significantly increased as compared to non-Hp 2-2 patients in all three subgroups of case–control (OR: 2.2, 95% CI: 1.3–3.6; P=0.003, cohort (OR: 1.3, 95% CI: 1.2–1.5; P=0.001 and randomized controlled trials (OR: 1.6, 1.2–2.2; P=0.005. Among patients with the Hp 2-2 genotype, administration of vitamin E was associated with lower rates of CV events (OR: 0.66, 95% CI: 0.45–0.95; P=0.025. Further investigation into the association between Hp

Pasteurization Procedures for Donor Human Milk Affect Body Growth, Intestinal Structure, and Resistance against Bacterial Infections in Preterm Pigs.

Science.gov (United States)

Li, Yanqi; Nguyen, Duc Ninh; de Waard, Marita; Christensen, Lars; Zhou, Ping; Jiang, Pingping; Sun, Jing; Bojesen, Anders Miki; Lauridsen, Charlotte; Lykkesfeldt, Jens; Dalsgaard, Trine Kastrup; Bering, Stine Brandt; Sangild, Per Torp

2017-06-01

Background: Holder pasteurization (HP) destroys multiple bioactive factors in donor human milk (DM), and UV-C irradiation (UVC) is potentially a gentler method for pasteurizing DM for preterm infants. Objective: We investigated whether UVC-treated DM improves gut maturation and resistance toward bacterial infections relative to HP-treated DM. Methods: Bacteria, selected bioactive components, and markers of antioxidant capacity were measured in unpasteurized donor milk (UP), HP-treated milk, and UVC-treated milk (all from the same DM pool). Fifty-seven cesarean-delivered preterm pigs (91% gestation; ratio of males to females, 30:27) received decreasing volumes of parental nutrition (average 69 mL · kg -1 · d -1 ) and increasing volumes of the 3 DM diets ( n = 19 each, average 89 mL · kg -1 · d -1 ) for 8-9 d. Body growth, gut structure and function, and systemic bacterial infection were evaluated. Results: A high bacterial load in the UP (6×10 5 colony forming units/mL) was eliminated similarly by HP and UVC treatments. Relative to HP-treated milk, both UVC-treated milk and UP showed greater activities of lipase and alkaline phosphatase and concentrations of lactoferrin, secretory immunoglobulin A, xanthine dehydrogenase, and some antioxidant markers (all P < 0.05). The pigs fed UVC-treated milk and pigs fed UP showed higher relative weight gain than pigs fed HP-treated milk (5.4% and 3.5%), and fewer pigs fed UVC-treated milk had positive bacterial cultures in the bone marrow (28%) than pigs fed HP-treated milk (68%) ( P < 0.05). Intestinal health was also improved in pigs fed UVC-treated milk compared with those fed HP-treated milk as indicated by a higher plasma citrulline concentration (36%) and villus height (38%) ( P < 0.05) and a tendency for higher aminopeptidase N (48%) and claudin-4 (26%) concentrations in the distal intestine ( P < 0.08). The gut microbiota composition was similar among groups except for greater proportions of Enterococcus in pigs

Clinical Comparative Study of the Effects of Helicobacter Pylori Colonization on Oral Health in Children.

Science.gov (United States)

Dane, Asim; Gurbuz, Taskin

2016-01-01

To isolate Helicobacter pylori (HP) from the dental plaque of a selected group of children and to compare the oral and salivary findings of patients with those of a healthy control group. A total of 70 children aged 5-15 years were included in this study. An intraoral examination was performed for each patient, and dental plaque and saliva specimens were collected for analysis. Oral health conditions, nutritional habits, tooth brushing frequency, saliva pH levels, flow velocity, and buffering capacities were noted. The Kruskal-Wallis test was used for comparison of the DMFT and dft index. The significance level was set at p=0.05. The prevalence of HP in dental plaque was higher in study group than controls (p0.05). Meanwhile, the buffering capacity of saliva was lower in HP gastritis patients (pdental plaque; thus, the oral cavity may be an important reservoir for HP. Good oral hygiene could be a positive contributor to the treatment of gastritis.

Clinical significance of measurement of serum IL-8, TNF-α and gastrin contents in helicobacter pylori associated digestive tract diseases

International Nuclear Information System (INIS)

Cao Jianfan; Ma Yunbao; Gao Leihua

2003-01-01

Objective: To investigate the relationship between the serum levels of IL-8, TNF-α, Gastrin and disease process in HP infection associated chronic gastritis and duodenal ulcer. Methods: Diagnosis was established in 35 HP positive chronic gastritis and 30 HP positive duodenal ulcer patients through gastroscopy, histopathology and 14 C-UBT test. RIA was adopted to measure the plasma contents of the three parameters in those 65 patients and 32 controls. Results: The serum IL-8, TNF-α and Gastrin (Gas) levels in patients with chronic gastritis were significantly higher than those in controls (p<0.01). Significant difference also existed between the contents in the two disease group (p<0.01). Conclusion: It proved that HP infection played an important role in the development of the two digestive tract diseases

Engineering description of the OMS/RCS/DAP modes used in the HP-9825A High Fidelity Relative Motion Program (HFRMP)

Science.gov (United States)

Wilson, S. W.

1978-01-01

Simplified mathematical models are reported for the space shuttle's Orbital Maneuvering System (OMS), Reaction Control System (RCS), and on-orbit Digital Autopilot (DAP) that have been incorporated in the High-Fidelity Relative Motion Program (HFRMP) for the HP-9825A desk-top calculator. Comparisons were made between data generated by the HFRMP and by the Space Shuttle Functional Simulator (SSFS), which models the cited shuttle systems in much greater detail. These data include propellant requirements for representative translational maneuvers, rotational maneuvers, and attitude maintenance options. Also included are data relating to on-orbit trajectory deviations induced by RCS translational cross coupling. Potential close-range stationkeeping problems that are suggested by HFRMP simulations of 80 millisecond (as opposed to 40 millisecond) DAP cycle effects are described. The principal function of the HFRMP is to serve as a flight design tool in the area of proximity operations.

Acute Phase Proteins and their Relation to Energy Metabolites in Dairy Cows during the Pre- and Postpartal Period

Directory of Open Access Journals (Sweden)

Gabriel Kováč

2009-01-01

Full Text Available The objective of this study was to evaluate the concentrations of selected acute phase proteins – haptoglobin (Hp and serum amyloid A (SAA, and selected variables of energy metabolism in blood serum of dairy cows during different stages of the pre- and postpartal period. The analyses were performed in dairy cows of the Slovak spotted breed and its crossbreeds (n = 57. The cows were divided into 9 groups according to the evaluated part of reproduction cycle – from 4 weeks before parturition to 10 weeks after parturition. Significant differences were found in mean Hp and SAA concentrations during the monitored period (P P P P P P P < 0.001. Moreover, significant correlations were found between Hp and non-esterified fatty acids, Hp and β-hydroxybutyrate, as well as between SAA and non-esterified fatty acids. Our results indicate that the acute phase response occurs in cows around parturition, and suggest that there are relationships between mediators of immune response and several indices of energy metabolism. Our results also suggest that in cows with higher concentrations of non-esterified fatty acids, indicating a certain degree of lipid mobilisation, higher values of acute phase proteins may be found.