Geothermal energy and hot springs in Ethiopia

Energy Technology Data Exchange (ETDEWEB)

Koga, T. (Hot Springs Therapeutics Research Institute, Kyushu, Univ., Japan)

1971-01-01

The hot springs in Ethiopia are concentrated in two areas: the North Afar depression and adjacent Red Sea shore, and a geothermal field 100 km from northeast to southwest in the central part of Ethiopia. The latter extends not only to the Great Rift Valley but also to the Aden Gulf. In the lake district in the central Great Rift Valley, there are a number of hot springs on the lake shore. These are along NE-SW fault lines, and the water is a sodium bicarbonate-type rich in HCO/sub 3/ and Na but low in C1 and Ca. In Dallol in the North Afar depression, CO/sub 2/-containing hot springs with high temperatures (110/sup 0/C) and a specific gravity of 1.4, were observed. In the South Afar depression, located in the northeastern part of the Rift Valley, there are many active volcanoes and hot springs between the lake district and the Danakil depression. The spring water is a sodium bicarbonate saline type. Nine graphs and maps are included.

Indoor radon levels in selected hot spring hotels in Guangdong, China

Energy Technology Data Exchange (ETDEWEB)

Song Gang [Guangzhou Institute of Geochemistry, Chinese Academy of Science, Guangzhou 510640 (China); Zhang Boyou [Guangzhou Institute of Geochemistry, Chinese Academy of Science, Guangzhou 510640 (China); Wang Xinming [Guangzhou Institute of Geochemistry, Chinese Academy of Science, Guangzhou 510640 (China)]. E-mail: wangxm@gig.ac.cn; Gong Jingping [Guangzhou Institute of Geochemistry, Chinese Academy of Science, Guangzhou 510640 (China); Chan, Daniel [Department of Building Services Engineering, Hong Kong Polytechnic University, Hong Kong (China); Bernett, John [Department of Building Services Engineering, Hong Kong Polytechnic University, Hong Kong (China); Lee, S.C. [Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hong Kong (China)

2005-03-01

Guangdong is one of the provinces that have most hot springs in China, and many hotels have been set up near hot springs, with spring water introduced into the bath inside each hotel room for hot spring bathing to attract tourists. In the present study, we measured radon in indoor and outdoor air, as well as in hot spring waters, in four hot spring hotels in Guangdong by using NR-667A (III) continuous radon detector. Radon concentrations ranged 53.4-292.5 Bq L{sup -1} in the hot spring water and 17.2-190.9 Bq m{sup -3} in outdoor air. Soil gas intrusion, indoor hot spring water use and inefficient ventilation all contributed to the elevated indoor radon levels in the hotel rooms. From the variation of radon levels in closed unoccupied hotel rooms, soil gas intrusion was found to be a very important source of indoor radon in hotel rooms with floors in contact with soils. When there was spring water bathing in the bathes, average radon levels were 10.9-813% higher in the hotel rooms and 13.8-489% higher in bathes compared to their corresponding average levels when there was no spring water use. Spring water use in the hotel rooms had radon transfer coefficients from 1.6x10{sup -4} to 5.0x10{sup -3}. Radon in some hotel rooms maintained in concentrations much higher than guideline levels might thus have potential health risks to the hotel workers, and technical and management measures should be taken to lower their exposure of radon through inhalation.

Report on the FY 1998 survey for preservation of Jozankei Hot Spring. Hot spring variation survey; 1998 nendo Jozankei onsen hozen chosa. Onsen hendo chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-09-01

Of the FY 1998 survey for preservation of Jozankei Hot Spring, a survey was conducted with the aim of grasping the state of variation in ingredients of hot spring, etc. in the area and of elucidating the causes of hot spring variation. During the period from October 27, 1998 to August 28, 1999, the following were carried out: sampling of specimens of spring water at 6 spring sources, river water at 2 points and precipitation at 2 points; measurement of temperature, spring temperature, pH, electric conductivity, etc.; analyses of Na, Ca, CL, HCO{sub 3}, SiO{sub 2}, etc. The results of the analysis are as follows. As to spring sources, A-2, A-7 and B-1, the precipitation or river water flow rate seem to largely affect the variation in hot spring measuring values. As to spring resources, A-6 and B-4, the relation with the precipitation or river water flow rate is not clear, but a big change is recognized in the snow-melting season. The tendency to the two variations seems to be caused by the difference between the spring with which the river water is greatly concerned by the crack system of the spring having reached the river and the spring which was closed on the earth surface. The temperature variation of springs was considered to be affected by the river water which flowed into the springs. (NEDO)

Field observations and management strategy for hot spring wastewater in Wulai area, Taiwan.

Science.gov (United States)

Lin, J Y; Chen, C F; Lei, F R; Hsieh, C D

2010-01-01

Hot springs are important centers for recreation and tourism. However, the pollution that may potentially be caused by hot spring wastewater has rarely been discussed. More than half of Taiwan's hot springs are located in areas where the water quality of water bodies is to be protected, and untreated wastewater could pollute the receiving water bodies. In this study, we investigate hot spring wastewater in the Wulai area, one of Taiwan's famous hot spring resorts. Used water from five hot spring hotels was sampled and ten sampling events were carried out to evaluate the changes in the quality of used water in different seasons, at different periods of the week, and from different types of hotels. The concentrations of different pollutants in hot spring wastewater were found to exhibit wide variations, as follows: COD, 10-250 mg/L; SS, N.D.-93 mg/L; NH(3)-N, 0.01-1.93 mg/L; TP, 0.01-0.45 mg/L; and E. coli, 10-27,500 CFU/100 mL. The quality of hot spring wastewater depends on the operation of public pools, because this affects the frequency of supplementary fresh water and the outflow volume. Two management strategies, namely, onsite treatment systems and individually packaged treatment equipment, are considered, and a multi-objective optimization model is used to determine the optimal strategy.

Hot springs in Hokuriku District

Energy Technology Data Exchange (ETDEWEB)

Sato, K. (Hot Springs Research Center, Japan)

1971-01-01

In the Hokuriku district including Toyama, Ishikawa, and Fukui Prefectures, hot springs of more than 25/sup 0/C were investigated. In the Toyama Prefecture, there are 14 hot springs which are located in an area from the Kurobe River to the Tateyama volcano and in the mountainous area in the southwest. In Ishikawa Prefecture there are 16 hot springs scattered in Hakusan and its vicinity, the Kaga mountains, and in the Noto peninsula. In northern Fukui Prefecture there are seven hot springs. The hot springs in Shirakawa in Gifu Prefecture are characterized as acid springs producing exhalations and H/sub 2/S. These are attributed to the Quaternary volcanoes. The hot springs of Wakura, Katayamazu, and Awara in Ishikawa Prefecture are characterized by a high Cl content which is related to Tertiary andesite. The hot springs of Daishoji, Yamanaka, Yamashiro, Kuritsu, Tatsunokuchi, Yuwaku, and Yunotani are characterized by a low HCO/sub 3/ content. The Ca and SO/sub 4/ content decreases from east to west, and the Na and Cl content increases from west to east. These fluctuations are related to the Tertiary tuff and rhyolite. The hot springs of Kuronagi, Kinshu, and Babadani, located along the Kurobe River are characterized by low levels of dissolved components and high CO/sub 2/ and HCO/sub 3/ content. These trends are related to late Paleozoic granite. Hot springs resources are considered to be connected to geothermal resources. Ten tables, graphs, and maps are provided.

New bathing therapy in Japanese hot springs using radiation from radon

International Nuclear Information System (INIS)

Sugimori, Kenji; Okajima, Maiko; Oowada, Mizuno; Koyama, Yoshihisa; Shozugawa, Katsumi; Matsuo, Motoyuki

2015-01-01

Japanese-style bathing is an important part of the traditional culture of Japan, and most Japanese people love hot springs. Many kinds of hot springs exist all over Japan and are often a major factor when considering where to go for travel, relaxation and rest. However, other countries, especially in Europe, also use hot springs for medical treatments such as balneo therapy, hydrokinetic therapy, fango therapy and inhalation therapy. Some hot springs in Japan are located on radioactive springs. Five typical radioactive spring areas can be found in Tamagawa (Akita Pref.), Murasugi (Niigata Pref.), Masutomi (Yamanashi Pref.), Misasa (Tottori Pref.), and Sekigane (Tottori Pref.). While hot springs in Japan are mainly used for bathing, these radioactive springs are also used for bedrock bathing and/or inhalation therapy. In Italy, Fango therapy is a medical treatment conducted under a medical doctor's super vision with peloids maturated with hot spring water called 'Fango'. Japanese style Fango, named Biofango R , has already been made by using natural hot springs that have been modified with Italian Fango. Medical evaluation of test subjects has shown good results after treatment with Fango therapy. An important point in Fango therapy is how to make satisfactory maturated peloids. For this purpose, an experiment was conducted at Masutomi hot spring to confirm the possibility of using radioactive spring water to make maturated peloids. The basement material for the peloids used for this experiment was made from bentonite mixed with original rock from the Masutomi hot spring area consisting of crushed basalt and granite that have a fine amount of radioactivity. These peloids were circulated through hot spring water for two weeks to a month and then used for treatment. The medical data showed that therapy using this method resulted in greater improvement in 'test subjects' body functions compared with the data from previous observations. This

Chemistry of Hot Spring Pool Waters in Calamba and Los Banos and Potential Effect on the Water Quality of Laguna De Bay

Science.gov (United States)

Balangue, M. I. R. D.; Pena, M. A. Z.; Siringan, F. P.; Jago-on, K. A. B.; Lloren, R. B.; Taniguchi, M.

2014-12-01

Since the Spanish Period (1600s), natural hot spring waters have been harnessed for balneological purposes in the municipalities of Calamba and Los Banos, Laguna, south of Metro Manila. There are at more than a hundred hot spring resorts in Brgy. Pansol, Calamba and Tadlac, Los Banos. These two areas are found at the northern flanks of Mt. Makiling facing Laguna de Bay. This study aims to provide some insights on the physical and chemical characteristics of hot spring resorts and the possible impact on the lake water quality resulting from the disposal of used water. Initial ocular survey of the resorts showed that temperature of the pool water ranges from ambient (>300C) to as high as 500C with an average pool size of 80m3. Water samples were collected from a natural hot spring and pumped well in Los Banos and another pumped well in Pansol to determine the chemistry. The field pH ranges from 6.65 to 6.87 (Pansol springs). Cation analysis revealed that the thermal waters belonged to the Na-K-Cl-HCO3 type with some trace amount of heavy metals. Methods for waste water disposal are either by direct discharge down the drain of the pool or by discharge in the public road canal. Both methods will dump the waste water directly into Laguna de Bay. Taking in consideration the large volume of waste water used especially during the peak season, the effect on the lake water quality would be significant. It is therefore imperative for the environmental authorities in Laguna to regulate and monitor the chemistry of discharges from the pool to protect both the lake water as well as groundwater quality.

Probability-based classifications for spatially characterizing the water temperatures and discharge rates of hot springs in the Tatun Volcanic Region, Taiwan.

Science.gov (United States)

Jang, Cheng-Shin

2015-05-01

Accurately classifying the spatial features of the water temperatures and discharge rates of hot springs is crucial for environmental resources use and management. This study spatially characterized classifications of the water temperatures and discharge rates of hot springs in the Tatun Volcanic Region of Northern Taiwan by using indicator kriging (IK). The water temperatures and discharge rates of the springs were first assigned to high, moderate, and low categories according to the two thresholds of the proposed spring classification criteria. IK was then used to model the occurrence probabilities of the water temperatures and discharge rates of the springs and probabilistically determine their categories. Finally, nine combinations were acquired from the probability-based classifications for the spatial features of the water temperatures and discharge rates of the springs. Moreover, various combinations of spring water features were examined according to seven subzones of spring use in the study region. The research results reveal that probability-based classifications using IK provide practicable insights related to propagating the uncertainty of classifications according to the spatial features of the water temperatures and discharge rates of the springs. The springs in the Beitou (BT), Xingyi Road (XYR), Zhongshanlou (ZSL), and Lengshuikeng (LSK) subzones are suitable for supplying tourism hotels with a sufficient quantity of spring water because they have high or moderate discharge rates. Furthermore, natural hot springs in riverbeds and valleys should be developed in the Dingbeitou (DBT), ZSL, Xiayoukeng (XYK), and Macao (MC) subzones because of low discharge rates and low or moderate water temperatures.

Neutron activation analysis of the rare earth elements in Nasu hot springs

International Nuclear Information System (INIS)

Ikeda, Nagao; Takahashi, Naruto.

1978-01-01

Eleven rare earth elements (lanthanum, cerium, neodymium, samarium, europium, gadolinium, terbium, holmium, thulium, ytterbium and lutetium) in hot spring waters and sinter deposits in the Nasu area were determined by the neutron activation method. The rare earth elements in hot spring water were preconcentrated in ferric hydroxide precipitate and neutron-irradiated. The rare earth elements were chemically separated into lighter and heavier groups and the activity of each group was measured with a Ge(Li) detector. Distribution of the rare earth elements between the hot spring water and the sinter deposit was also discussed. (auth.)

Biomineralization of radioactive sulfide minerals in strong acidic Tamagawa hot springs

International Nuclear Information System (INIS)

Tazaki, Kazue; Watanabe, Hiroaki

2004-01-01

Bioaccumulation of radioactive sulfide minerals by bacteria in strong acidic hot spring water was found at Tamagawa Hot Springs, Akita prefecture in Japan. The hot spring water produces Hokutolite of radioactive minerals high radium and radon. The β-ray measurements of sediments and biofilms indicate 1850-2420 and 5700 cpm, respectively, which are 50-100 times higher than that of the water and the air (50-90 cpm). The characteristics of hot spring water show pH (1.2), Eh (140 mV), EC (29 mS/cm), DO (0.8 mg/l), and water temperature (99.5degC), indicating extremely strong acidic and reducing conditions. The hot spring water contains mainly HCl associated with high concentrations of Ca 2+ , Al 3+ , Fe 2+ , HSO 4 - and SO 4 2- . SEM-EDX and TEM demonstrate some insight into how microorganisms affect the chemistry and microbiological characteristics of the strong acidic surroundings with high S, As, Ba, and Ca contents in biofilms. Especially SEM-EDX, ED-XRF, and STEM-EDX elemental content maps illustrate the distribution of sulfur-bearing compounds of barite (BaSO 4 ), gypsum (CaSO 4 ·2H 2 O), elemental sulfur (S) and orpiment(As 2 S 3 ) in the reddish orange biofilms. The presence of a hydrogen sulfide-rich (H 2 S) thermal spring and gypsum deposits suggest the volatilization of H 2 S from the spring water, oxidation of the H 2 S gas to sulfuric acid, and reaction of the sulfuric acid. TEM micrographs of bacteria in the biofilms reveal in detail the intimate connections between biological and mineralogical processes that the cells are entirely accumulated with spherical grains, 100∼200 nm in diameter. The relationship among sulfide minerals, such as barite, gypsum, sulfur, orpiment, and Hakutolite, associated with bacteria implies that heavy metals have been transported from strong acidic hot spring water to sediments through bacteria metabolism. It is possible that the capability of radioactive sulfide biofilms for heavy metal immobilization can be used to

Chemical analyses of waters from geysers, hot springs, and pools in Yellowstone National Park, Wyoming from 1974 to 1978

Energy Technology Data Exchange (ETDEWEB)

Thompson, J.M.; Yadav, S.

1979-01-01

Waters from geysers, hot springs, and pools of Yellowstone National Park have been analyzed. We report 422 complete major ion analyses from 330 different locations of geysers, hot springs, and pools, collected from 1974 to 1978. Many of the analyses from Upper, Midway, Lower, and Norris Geyser Basin are recollections of features previously reported.

Microbiology of Kamchatka Peninsula Hot Springs

Science.gov (United States)

Bonch-Osmolovsk, E.

2005-12-01

Hot springs of Uzon Caldera, Geyser Valley, Moutnovsky Volcano (Kamchatka Peninsula) served as the sources of isolation of numerous thermophilic prokaryotes, many of them representing new taxa. Among new isolates there were hyperthermophilic archaea - neutrophilic or acidophilic anaerobic organotrophs, able to use a wide range of polymeric organic substrates. Bacterial isolates were in majority represented by moderate thermophiles - organotrophs and lithoautotrophs. Latter group consisted of anaerobes oxidizing molecular hydrogen in the course of sulfate, sulfur or iron reduction, and of anaerobic CO-oxidizing, hydrogen-producing bacteria. Some of new isolates represented deep phylogenetic lineages in Bacteria domain. Microbial activity in Kamchatka hot springs was studied by means of radioisotopic tracing. The rates of methanogenesis, acetogenesis, inorganic carbon assimilation, acetate oxidation were determined in three different hot springs with pH ranging from 3.0 to 8.5 and water temeperature being in the range from 55 to 85oC. The results indicated the presence and activity of novel metabolic groups of thermophilic prokaryotes that so far have not been known in laboratory cultures.

Fiscal 1999 survey on conservation of Jozankei hot spring. Survey report of hot spring alteration; 1999 nendo jozankei onsen hozen chosa. Onsen hendo chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-06-01

As a part of fiscal 1999 survey on conservation of Jozankei hot spring, a survey was made on the change of water composition in hot springs and rivers for the purpose of elucidating the cause of hot spring alteration. In the survey, sampling and water quality analysis were conducted once a month at four sources and one river point on the upstream side of Tsukimibashi bridge on the Toyohira river and at two sources and one river point between Tsukimibashi bridge and Takayamabashi bridge. Also carried out were sampling and constituent analysis at five points for river water of a wide area. The survey results were as follows. The pattern of change in spring water temperature was recognized such that it tended to rise in summer when river temperature was high and to fall in winter. Spring water temperature rose greatly at the time of higher water level like the thaw in some sources but conversely fell in other sources. The fluctuation trend in PH values was such that they mostly went up in April of snow melting time and in early August of much rain and went down in winter of little rain and less snowmelt. As for electric conductivity and dissolved constituents, it was recognized that the conductivity lowered and that the constituents decreased in concentration, all concurrently at the time of snowmelt and much rain. (NEDO)

Report on fiscal 1998 investigation of Jozankei hot spring conservation and hot spring structure; 1998 nendo Jozankei onsen hozen chosa. Onsen kozo chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-10-01

With the purpose of evaluating recoverable hot water quantity and elucidating the change over a long term, investigations were carried out, with the results summarized, on the geology, alteration zone, gravitational analysis, fluid geochemistry and hydraulics in the area. The investigations covered the area of 7 km x 6 km in about 30 km southwest of Sapporo City and were performed for the period from September 10, 1998 to October 31, 1999. The results were as follows. In the Jozankei area, with the Usubetu layer in the Old Tertiary system as the basement, layers are superposed from the Palaeogene Oligocene to the Quaternary Pleistocene. Distributing in various places between Yunosawa vicinity and Jozankei Hot Spring area are acid to neutral geothermal alteration zones. The hot spring gushing-out zone in the Jozankei hot spring area is supposed to be regulated by side-by-side cracks in the NE-SW direction. It was inferred from tritium concentration and a minor component ratio that, as the mechanism of forming a hot spring, water of precipitation origin circulating and residing for a long time on the Usubetsu layer which is marine sediment is heated by a volcanic heat source latent in the depth. (NEDO)

Biomediated Precipitation of Calcium Carbonate in a Slightly Acidic Hot Spring

Science.gov (United States)

Jiang, L.

2015-12-01

A slightly acidic hot spring named "Female Tower" (T=73.5 °C, pH=6.64) is located in the Jifei Geothermal Field, Yunnan Province, Southwest China. The precipitates in the hot spring are composed of large amounts of calcite, aragonite, and sulfur. Scanning electron microscopy (SEM) analyses revealed that the microbial mats were formed of various coccoid, rod-shaped, and filamentous microbes. Transmission electron microscopy (TEM) showed that the intracellular sulfur granules were commonly associated with these microbes. A culture-independent molecular phylogenetic analysis demonstrated that the majority of the bacteria in the spring were sulfur-oxidizing bacteria. In the spring water, H2S concentration was up to 60 ppm, while SO42- concentration was only about 10 ppm. We speculated that H2S might be utilized by sulfur-oxidizing bacteria in this hot spring water, leading to the intracellular formation of sulfur granules. In the meantime, this reaction increased the pH in the micron-scale microdomains, which fostered the precipitation of calcium carbonate in the microbial mats. The results of this study indicated that the sulfur-oxidizing bacteria could play an important role in calcium carbonate precipitation in slightly acidic hot spring environments.

Determination of arsenic and bromine in hot spring waters by neutron activation analysis

International Nuclear Information System (INIS)

Kikawada, Y.; Kawai, S.; Oi, T.

2004-01-01

Concentrations of arsenic and bromine dissolved in hot spring waters have been determined by neutron activation analysis using 0.5 cm 3 of sample waters without any chemical pretreatment. The samples prepared for neutron irradiation were simply pieces of filter papers which were infiltrated with samples. With the results of satisfactorily high accuracy and precision, this analytical method was found to be very convenient for the determinations of arsenic and bromine dissolved in water at ppm to sub-ppm levels. (author)

Isotopic and chemical features of hot springs in Akita Prefecture

International Nuclear Information System (INIS)

Matsubaya, Osamu

1997-01-01

All over the Akita Prefecture, many hot springs are located. Most of them are of meteoric water, fossil sea water and volcanic gas origins. In the Ohdate-Kazuno area, moderate temperature hot springs of meteoric water origin are found, which may exist as rather shallow formation water in the Green Tuff formations. On the contrary, high temperature geothermal waters of meteoric origin, which are used for power generation, are obtained in two volcanic area of Hachimantai and Oyasu. Those geothermal waters are expected to come up through vertical fissures from depth deeper than 2 km. The difference of these two manners of meteoric water circulation should be necessarily explained to understand the relationship of shallow and deep geothermal systems. About some hot springs of fossil sea water origin, the relationships of δ D and Cl - don't agree to the mixing relation of sea water and meteoric water. This may be explained by two different processes, one of which is mixing of sea water with saline meteoric water (Cl - ca. 12 g/kg). The other is modification of δD by hydrogen isotopic exchange with hydrous minerals underground, or by exchange with atmospheric vapor during a relic lake before burying. (author)

Geochemical studies of Ishiwa hot springs in Yamanashi Prefecture-yearly change of hot springs

Energy Technology Data Exchange (ETDEWEB)

Akiyama, T. (Yamanashi Prefecture Womens Junior College, Japan)

1971-12-01

The effect of drilling on the Ishiwa hot springs was studied. About 50 wells have been drilled since 1961 when the first well was drilled to a depth of 146 m where 47/sup 0/C water flowed at 1376 l/min. Changes have occurred in flow rate, temperature, and chemical composition of the spring water. In area A near the foot of northern Okura-Keijisan along the Byodo and Fuefuki rivers, the pH value was 8.0 to 8.2 when drilling began, but it is now 7.4 to 8.0. In area B in the central spring area along the Chikatsu water reservoir, the pH was about 8.5 when drilling began, but is presently 10. The shift of area A pH to acidic is thought to be due to the effect of river water. The shift in area B pH to alkaline was thought to be connected to the fountainhead with pH 10 which appeared in the Kami-Ogihara Resseki district in Shioyama City. The fountainhead was located along the Fuefuki River at the foot of Obosatsu, 20 km from the Ishiwa area.

Geothermal Exploration in Hot Springs, Montana

Energy Technology Data Exchange (ETDEWEB)

Toby McIntosh, Jackola Engineering

2012-09-26

The project involves drilling deeper in the Camp Aqua well dri lled in June 1982 as part of an effort to develop an ethanol plant. The purpose of the current drill ing effort is to determine if water at or above 165°F exists for the use in low temperature resource power generation. Previous geothermal resource study efforts in and around Hot Springs , MT and the Camp Aqua area (NE of Hot Springs) have been conducted through the years. A confined gravel aquifer exists in deep alluvium overlain by approximately 250 of si lt and c lay deposits from Glacial Lake Missoula. This gravel aquifer overlies a deeper bedrock aquifer. In the Camp Aqua area several wel l s exist in the gravel aquifer which receives hot water f rom bedrock fractures beneath the area. Prior to this exploration, one known well in the Camp Aqua area penetrated into the bedrock without success in intersecting fractures transporting hot geothermal water. The exploration associated with this project adds to the physical knowledge database of the Camp Aqua area. The dri l l ing effort provides additional subsurface information that can be used to gain a better understanding of the bedrock formation that i s leaking hot geothermal water into an otherwise cold water aquifer. The exi s t ing well used for the explorat ion is located within the center of the hottest water within the gravel aquifer. This lent i t sel f as a logical and economical location to continue the exploration within the existing well. Faced with budget constraints due to unanticipated costs, changing dril l ing techniques stretched the limited project resources to maximize the overa l l well depth which f e l l short of original project goals. The project goal of finding 165°F or hotter water was not achieved; however the project provides additional information and understanding of the Camp Aqua area that could prove valuable in future exploration efforts

Effects of normal saline and selenium-enriched hot spring water on experimentally induced rhinosinusitis in rats.

Science.gov (United States)

Kim, Dong-Hyun; Yeo, Sang Won

2013-01-01

This prospective, randomized, and controlled study examined the effects of normal saline and selenium-enriched hot spring water on experimentally induced rhinosinusitis in rats. The study comprised two control groups (untreated and saline-treated) and three experimental groups of Sprague Dawley rats. The experimental groups received an instillation of lipopolysaccharide (LPS) only, LPS+normal saline (LPS/saline), or LPS+selenium-enriched hot spring water (LPS/selenium). Histopathological changes were identified using hematoxylin-eosin staining. Leakage of exudate was identified using fluorescence microscopy. Microvascular permeability was measured using the Evans blue dye technique. Expression of the Muc5ac gene was measured using reverse transcription-polymerase chain reaction. Mucosal edema and expression of the Muc5ac gene were significantly lower in the LPS/saline group than in the LPS group. Microvascular permeability, mucosal edema, and expression of the Muc5ac gene were significantly lower in the LPS/selenium group than in the LPS group. Mucosal edema was similar in the LPS/selenium group and LPS/saline group, but capillary permeability and Muc5ac expression were lower in the LPS/selenium group. This study shows that normal saline and selenium-enriched hot spring water reduce inflammatory activity and mucus hypersecretion in LPS-induced rhinosinusitis in rats. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

A study to evaluate therapeutic properties of minerals of manghopir hot spring, karachi

International Nuclear Information System (INIS)

Javed, A.; Iqbal, J.; Khan, F.A.; Siddiqui, I.

2009-01-01

European balneologists have extensively studied the therapeutic value of mineral water. Mineral springs with different mineral contents are recommended for various therapeutic uses. People have been using geothermal water for bathing and good health for many thousands of years A mineral hot spring has greater than 1000 mg/L (ppm) of naturally dissolved solids. Hot mineral spring water contains elements like calcium, magnesium, sodium, potassium as sulphates, bi- carbonates and chlorides, which are used externally to cure many diseases. Manghopir spring contain 38-84 mg/L calcium, 29-56 mg/L magnesium, 388-555 mg/L sodium, 411-599 mg/L chloride, 186-442 mg/L sulphate, 10-25 mg/L potassium, and 1509-2188 mg/L total dissolved solids while the pH was in the range of 7.2-7.8. The temperature of Manghopir Euthermal hot spring remains constant ranging between 40 to 47 degree C. (author)

A preliminary survey of radon concentrations in South Island hot springs

International Nuclear Information System (INIS)

Whitehead, N.E.

1976-02-01

Radon 222 was determined in hot spring waters from the South Island of New Zealand by a method involving the radiochemical isolation of 214 Bi. The results ranged from 137 to 1830 pCi/l with a mean of 738 pCi/l. These results are lower than those reported in the literature for North Island hot springs. (auth.)

Fiscal 1999 survey report on Jozankei Hot Spring conservation (3rd phase); 1999 nendo Jozankei onsen hozen chosa hokokusho (dai sanji)

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-11-01

The impact of geothermal exploitation in the Yunosawa district on the Jozankei hot spring and others in the neighborhood was evaluated, and a survey was conducted of the formation and eruption mechanisms of the Jozankei hot spring for the purpose of hot spring conservation. Activities were conducted in the three fields of (1) geological structure analysis, (2) geochemical analysis of fluids, and (3) comprehensive analysis. Conducted in field (2) were analysis of hot spring utilization data and the contents, analysis of hot spring water and geothermal water, analysis of fluctuations in hot springs, and fluid movement models. Studied in field (3) were the outline of large area geothermal systems, geothermal structure models, relations between geothermal reservoirs and hot spring aquifers, and impact of geothermal exploitation on hot springs. Disclosed as the result were hot spring geological structure models, formation mechanism, eruption mechanism, origins of hot spring water, fluid movement models, interference between hot spring units, and changes in the hot springs as a whole. It was then concluded that the geothermal exploitation in the Yunosawa district would not exert any impact on the hot springs. (NEDO)

Contribution of 222Rn-bearing water to indoor radon and indoor air quality assessment in hot spring hotels of Guangdong, China

International Nuclear Information System (INIS)

Song Gang; Wang Xinming; Chen Diyun; Chen Yongheng

2011-01-01

This study investigates the contribution of radon ( 222 Rn)-bearing water to indoor 222 Rn in thermal baths. The 222 Rn concentrations in air were monitored in the bathroom and the bedroom. Particulate matter (PM, both PM 10 and PM 2.5 ) and carbon dioxide (CO 2 ) were also monitored with portable analyzers. The bathrooms were supplied with hot spring water containing 66-260 kBq m -3 of 222 Rn. The results show that the spray of hot spring water from the bath spouts is the dominant mechanism by which 222 Rn is released into the air of the bathroom, and then it diffuses into the bedroom. Average 222 Rn level was 110-410% higher in the bedrooms and 510-1200% higher in the bathrooms compared to the corresponding average levels when there was no use of hot spring water. The indoor 222 Rn levels were influenced by the 222 Rn concentrations in the hot spring water and the bathing times. The average 222 Rn transfer coefficients from water to air were 6.2 x 10 -4 -4.1 x 10 -3 . The 24-h average levels of CO 2 and PM 10 in the hotel rooms were 89% and 22% higher than the present Indoor Air Quality (IAQ) standard of China. The main particle pollutant in the hotel rooms was PM 2.5 . Radon and PM 10 levels in some hotel rooms were at much higher concentrations than guideline levels, and thus the potential health risks to tourists and especially to the hotel workers should be of great concern, and measures should be taken to lower inhalation exposure to these air pollutants. - Highlights: → 222 Rn-bearing water is the main contributor to indoor radon in hot spring hotel. → The PM 2.5 and CO 2 are also the main indoor pollutants in the hotel rooms. → Higher radon and PM levels might have significant negative health effects to human. → The radon transfer coefficients are consistent with the published data.

Toxic cyanobacteria and cyanotoxins in public hot springs in Saudi Arabia.

Science.gov (United States)

Mohamed, Zakaria A

2008-01-01

Toxic cyanobacteria are well reported in rivers, lakes and even marine environments, but the toxin production of cyanobacteria in hot springs is largely unexplored. Therefore, the present study investigated the presence of toxic cyanobacteria and cyanotoxins in public hot springs in Saudi Arabia. The results of an enzyme-linked immunosorbent assay (ELISA) revealed that Saudi spring cyanobacterial mats contained microcystins (MCYSTs) at concentrations ranging from 468 to 512.5 microg g(-1). The Limulus amebocyte lystae (LAL) assay detected lipopolysaccharide (LPS) endotoxins in these mats at concentrations ranging from 433.3 to 506.8 EU g(-1). MCYSTs and endotoxins were also detected in spring waters at levels of 5.7 microg l(-1) and 640 EU ml(-1), respectively, exceeding WHO's provisional guideline value for MCYST-LR in drinking-water. High-performance liquid chromatography (HPLC) analysis revealed that only Oscillatoria limosa and Synechococcus lividus can produce MCYSTs with a profile consisting of MCYST-RR and -LR. Based on the LAL assay, 12 out of 17 cyanobacterial species contained LPS at concentrations ranging from 0.93 to 21.06 EU g(-1). However, not all LPS of these species were toxic to mice. This study suggests that the hot springs in the world including Saudi Arabia should be screened for toxic cyanobacteria to avoid the exposure of people recreating and bathing in spring waters to cyanobacterial toxins.

A radioecological survey of eatable organisms for natural radionuclides in hot spring water

International Nuclear Information System (INIS)

Zhu, H.; Huang, X.; Song, H.; Li, J.; Zhang, J.

1993-01-01

This paper reports a radioecological survey on some aquatic eatable organisms raised in a hot spring water, which is rich in 226 Ra, in Hubei Province; and on agricultural products irrigated with the water. The contents of 226 Ra, 210 Pb and 210 Po in the water, some aquatic organisms, rice, vegetable an some other connected environmental samples were determined. The Concentration Factor (CF) or Transfer Coefficient (TC) from environmental medium into the eatable parts of the organisms for these nuclides as well as relative Distribution Factor (DF) was calculated. (author). 6 refs, 1 fig., 9 tabs

Hydrosalinity studies of the Virgin River, Dixie Hot Springs, and Littlefield Springs, Utah, Arizona, and Nevada

Science.gov (United States)

Gerner, Steven J.; Thiros, Susan A.; Gerner, Steven J.; Thiros, Susan A.

2014-01-01

The Virgin River contributes a substantial amount of dissolved solids (salt) to the Colorado River at Lake Mead in the lower Colorado River Basin. Degradation of Colorado River water by the addition of dissolved solids from the Virgin River affects the suitability of the water for municipal, industrial, and agricultural use within the basin. Dixie Hot Springs in Utah are a major localized source of dissolved solids discharging to the Virgin River. The average measured discharge from Dixie Hot Springs during 2009–10 was 11.0 cubic feet per second (ft3/s), and the average dissolved-solids concentration was 9,220 milligrams per liter (mg/L). The average dissolved-solids load—a measurement that describes the mass of salt that is transported per unit of time—from Dixie Hot Springs during this period was 96,200 tons per year (ton/yr). Annual dissolved-solids loads were estimated at 13 monitoring sites in the Virgin River Basin from streamflow data and discrete measurements of dissolved-solids concentrations and (or) specific conductance. Eight of the sites had the data needed to estimate annual dissolved-solids loads for water years (WYs) 1999 through 2010. During 1999–2010, the smallest dissolved-solids loads in the Virgin River were upstream of Dixie Hot Springs (59,900 ton/yr, on average) and the largest loads were downstream of Littlefield Springs (298,200 ton/yr, on average). Annual dissolved-solids loads were smallest during 2002–03, which was a period of below normal precipitation. Annual dissolved-solids loads were largest during 2005—a year that included a winter rain storm that resulted in flooding throughout much of the Virgin River Basin. An average seepage loss of 26.7 ft3/s was calculated from analysis of monthly average streamflow from July 1998 to September 2010 in the Virgin River for the reach that extends from just upstream of the Utah/Arizona State line to just above the Virgin River Gorge Narrows. Seepage losses from three river reaches

A search for correlation between seismicity and radon anomaly in hot springs

International Nuclear Information System (INIS)

Amin, B.S.; Rama

1982-01-01

Measurements of radon contents of the exholved gas emanating from several hot water springs along the Western Coast of India are reported here. Concentration of radon in gas phase of individual sprinqs varied in general, directly with the surface temperature of the water emerging from the respective springs, and showed little variation with time. Radon measurements were carried out continuously for about two years at two hot springs located at Ganeshpuri and Sathivali in the coastal area of Northern Maharashtra. The distant tremors did not cause any variation in the radon content. There was no marked local seismic activity during the period of observations, and the levels of radon stayed essentially constant. The measurements were also carried out at a hot spring in Assam, for about 8 months. These also did not show any significant variation; this period too lacked any marked local seismicity. (author)

Radiological Studies in the Hot Spring Region of Oyoun Mossa and Hammam Faraun Thermal Spring Areas in Western Sinai

International Nuclear Information System (INIS)

Ramadan, Kh.A.; Badran, H.M.; Ramadan, Kh.A.; Seddeek, M.K.; Sharshar, T.; Sharshar, T.

2009-01-01

Radioactivity in and around the two hot springs, Oyoun Mossa and Hammam Faraun, Western Sinai has been determined. The ground water, sediment and sand samples were measured by gamma-ray spectrometer for 232 Th, 226 Ra and 40 K isotopes. The enrichment of 226 Ra in Hammam Faraun hot spring was the most prominent feature. The concentration of 226 Ra in Oyoun Mossa and Hammam Faraun hot springs are 68 and 2377 Bq/kg for sediments, 3.5 and 54.7 Bq/kg for wild plants, and 205 and 1945 mBq/l for the ground water, respectively. In addition, the concentration of sand samples are 14 times larger in the area of Hammam Faraun compared with that of Oyoun Mossa. On the other hand, the concentration of 232 Th in different samples are comparable in the two areas while 137 Cs concentrations are relatively higher in Oyoun Mossa. For the purpose of comparison, sand samples were collected from two locations 5-12 km away from each spring. The activity concentrations of the four locations are comparable and in agreement with those from the area of the two springs except in one case. The major difference was the activity concentration of 226 Ra in the area of Hammam Faraun, which is much higher. The concentrations of all detected isotopes in water samples from these two springs are much higher than that detected in 27 natural wells in north Sinai. The results of the present study indicate that water only in Hammam Faraun hot spring is contaminated with 238 U-isotopes and the surrounding area is affected by this contamination. The calculated annual effective dose equivalents in the surroundings of Hammam Faraun (81.8 μSv) is superior to the maximum contaminant levels recommended.

Characteristics and Origins of Hot Springs in the Tatun Volcano Group in Northern Taiwan

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Chia-Mei Liu

2011-01-01

Full Text Available This paper systematically surveyed distribution and field occurrences of 13 hot springs as well as geochemical investigation on the geothermal area of the Tatun Volcano Group (TVG. According to Piper diagrams, pH values, field occurrences and water-rock interactions, these hot springs can be classified into three types: (1 Type I, SO42- acidic water where the reservoir is located in the Wuchishan Formation; (2 Type II, HCO3- a near neutral spring where waters originate from the volcanic terrane (andesite; and (3 Type III, Cl- -rich acidic water where waters emanate from shallower Wuchishan Formation. In terms of isotopic ratio, δD and δ18O values, two groups of hot spring can be recognized. One is far away from the meteoric water line of the Tatun area with values ranging between -26.2‰ and -3.5‰, and from -3.2‰ to 1.6‰, respectively. However, another close to the meteoric water line of the Tatun area is between -28.4‰ and -13.6‰, and from -5.5‰ to -4.2‰, respectively. In addition, the δ34S value of thermal waters can also be distinguished into two groups, one ranging from 26.1‰ to 28.5‰, and the other between 0.8‰ and 7.8‰. Based on field occurrences and geochemical characteristics, a model has been proposed to illustrate the origin of these hot springs.

Archaeal Nitrification in Hot Springs

Science.gov (United States)

Richter, A.; Daims, H.; Reigstad, L.; Wanek, W.; Wagner, M.; Schleper, C.

2006-12-01

Biological nitrification, i.e. the aerobic conversion of ammonia to nitrate via nitrite, is a major component of the global nitrogen cycle. Until recently, it was thought that the ability to aerobically oxidize ammonia was confined to bacteria of the phylum Proteobacteria. However, it has recently been shown that Archaea of the phylum Crenarchaeota are also capable of ammonia oxidation. As many Crenarchaeota are thermophilic or hyperthermophilic, and at least some of them are capable of ammonia oxidation we speculated on the existence of (hyper)thermophilic ammonia-oxidizing archaea (AOA). Using PCR primers specifically targeting the archaeal ammonia monooxygenase (amoA) gene, we were indeed able to confirm the presence of such organisms in several hot springs in Reykjadalur, Iceland. These hot springs exhibited temperatures well above 80 °C and pH values ranging from 2.0 to 4.5. To proof that nitrification actually took place under these extreme conditions, we measured gross nitrification rates by the isotope pool dilution method; we added 15N-labelled nitrate to the mud and followed the dilution of the label by nitrate production from ammonium either in situ (incubation in the hot spring) or under controlled conditions in the laboratory (at 80 °C). The nitrification rates in the hot springs ranged from 0.79 to 2.22 mg nitrate-N per L of mud and day. Controls, in which microorganisms were killed before the incubations, demonstrated that the nitrification was of biological origin. Addition of ammonium increased the gross nitrification rate approximately 3-fold, indicating that the nitrification was ammonium limited under the conditions used. Collectively, our study provides evidence that (1) AOA are present in hot springs and (2) that they are actively nitrifying. These findings have major implications for our understanding of nitrogen cycling of hot environments.

Global occurrence of archaeal amoA genes in terrestrial hot springs.

Science.gov (United States)

Zhang, Chuanlun L; Ye, Qi; Huang, Zhiyong; Li, Wenjun; Chen, Jinquan; Song, Zhaoqi; Zhao, Weidong; Bagwell, Christopher; Inskeep, William P; Ross, Christian; Gao, Lei; Wiegel, Juergen; Romanek, Christopher S; Shock, Everett L; Hedlund, Brian P

2008-10-01

Despite the ubiquity of ammonium in geothermal environments and the thermodynamic favorability of aerobic ammonia oxidation, thermophilic ammonia-oxidizing microorganisms belonging to the crenarchaeota kingdom have only recently been described. In this study, we analyzed microbial mats and surface sediments from 21 hot spring samples (pH 3.4 to 9.0; temperature, 41 to 86 degrees C) from the United States, China, and Russia and obtained 846 putative archaeal ammonia monooxygenase large-subunit (amoA) gene and transcript sequences, representing a total of 41 amoA operational taxonomic units (OTUs) at 2% identity. The amoA gene sequences were highly diverse, yet they clustered within two major clades of archaeal amoA sequences known from water columns, sediments, and soils: clusters A and B. Eighty-four percent (711/846) of the sequences belonged to cluster A, which is typically found in water columns and sediments, whereas 16% (135/846) belonged to cluster B, which is typically found in soils and sediments. Although a few amoA OTUs were present in several geothermal regions, most were specific to a single region. In addition, cluster A amoA genes formed geographic groups, while cluster B sequences did not group geographically. With the exception of only one hot spring, principal-component analysis and UPGMA (unweighted-pair group method using average linkages) based on the UniFrac metric derived from cluster A grouped the springs by location, regardless of temperature or bulk water pH, suggesting that geography may play a role in structuring communities of putative ammonia-oxidizing archaea (AOA). The amoA genes were distinct from those of low-temperature environments; in particular, pair-wise comparisons between hot spring amoA genes and those from sympatric soils showed less than 85% sequence identity, underscoring the distinctness of hot spring archaeal communities from those of the surrounding soil system. Reverse transcription-PCR showed that amoA genes were

Distribution of sequence-based types of legionella pneumophila serogroup 1 strains isolated from cooling towers, hot springs, and potable water systems in China.

Science.gov (United States)

Qin, Tian; Zhou, Haijian; Ren, Hongyu; Guan, Hong; Li, Machao; Zhu, Bingqing; Shao, Zhujun

2014-04-01

Legionella pneumophila serogroup 1 causes Legionnaires' disease. Water systems contaminated with Legionella are the implicated sources of Legionnaires' disease. This study analyzed L. pneumophila serogroup 1 strains in China using sequence-based typing. Strains were isolated from cooling towers (n = 96), hot springs (n = 42), and potable water systems (n = 26). Isolates from cooling towers, hot springs, and potable water systems were divided into 25 sequence types (STs; index of discrimination [IOD], 0.711), 19 STs (IOD, 0.934), and 3 STs (IOD, 0.151), respectively. The genetic variation among the potable water isolates was lower than that among cooling tower and hot spring isolates. ST1 was the predominant type, accounting for 49.4% of analyzed strains (n = 81), followed by ST154. With the exception of two strains, all potable water isolates (92.3%) belonged to ST1. In contrast, 53.1% (51/96) and only 14.3% (6/42) of cooling tower and hot spring, respectively, isolates belonged to ST1. There were differences in the distributions of clone groups among the water sources. The comparisons among L. pneumophila strains isolated in China, Japan, and South Korea revealed that similar clones (ST1 complex and ST154 complex) exist in these countries. In conclusion, in China, STs had several unique allelic profiles, and ST1 was the most prevalent sequence type of environmental L. pneumophila serogroup 1 isolates, similar to its prevalence in Japan and South Korea.

Surveillance of Vittaforma corneae in hot springs by a small-volume procedure.

Science.gov (United States)

Chen, Jung-Sheng; Hsu, Tsui-Kang; Hsu, Bing-Mu; Huang, Tung-Yi; Huang, Yu-Li; Shaio, Men-Fang; Ji, Dar-Der

2017-07-01

Vittaforma corneae is an obligate intracellular fungus and can cause human ocular microsporidiosis. Although accumulating reports of V. corneae causing keratoconjunctivitis in both healthy and immunocompromised persons have been published, little is known about the organism's occurrence in aquatic environments. Limitations in detection sensitivity have meant a large sampling volume is required to detect the pathogen up to now, which is problematic. A recent study in Taiwan has shown that some individuals suffering from microsporidial keratitis (MK) were infected after exposure to the pathogen at a hot spring. As a consequence of this, a survey and analysis of environmental V. corneae present in hot springs became an urgent need. In this study, sixty water samples from six hot spring recreation areas around Taiwan were analyzed. One liter of water from each sample site was filtered to harvest the fungi. The positive samples were detected using a modified nested PCR approach followed by sequencing using specific SSU rRNA gene primer pairs for V. corneae. In total fifteen V. corneae-like isolates were identified (25.0% of sites). Among them, six isolates, which were collected from recreational areas B, C and D, were highly similar to known V. corneae keratitis strains from Taiwan and other countries. Furthermore, five isolates, which were collected from recreation areas A, C, E and F, were very similar to Vittaforma-like diarrhea strains isolated in Portugal. Cold spring water tubs and public foot bath pools had the highest detection rate (50%), suggesting that hot springs might be contaminated via untreated water sources. Comparing the detection rate across different regions of Taiwan, Taitung, which is in the east of the island, gave the highest positive rate (37.5%). Statistical analysis showed that outdoor/soil exposure and a high heterotrophic plate count (HPC) were risk factors for the occurrence of V. corneae. Our findings provide empirical evidence

Silicon isotope fractionation during silica precipitation from hot-spring waters

Science.gov (United States)

Geilert, Sonja; Vroon, Pieter; Keller, Nicole; Gudbrnadsson, Snorri; Stefánsson, Andri; van Bergen, Manfred

2014-05-01

Hot-spring systems in the Geysir geothermal area, Iceland, have been studied to explore silicon isotope fractionation in a natural setting where sinter deposits are actively formed over a temperature interval between 20° and 100° C. The SiO2(aq)concentrations in spring and stream waters range between 290 and 560ppm and stay relatively constant along downstream trajectories, irrespective of significant cooling gradients. The waters are predominantly oversaturated in amorphous silica at the temperatures measured in the field. Correlations between the saturation indices, temperature and amounts of evaporative water loss suggest that cooling and evaporation are the main causes of subaqueous silica precipitation. The δ30Si values of dissolved silica in spring water and outflowing streams average around +1o probably due to the small quantities of instantaneously precipitating silica relative to the dissolved amount. Siliceous sinters, in contrast, range between -0.1o to -4.0o consistent with a preferred incorporation of the light silicon isotope and with values for precipitated silica becoming more negative with downstream decreasing temperatures. Larger fractionation magnitudes are inversely correlated with the precipitation rate, which itself is dependent on temperature, saturation state and the extent of a system. The resulting magnitudes of solid-fluid isotopic fractionation generally decline from -3.5o at 10° C to -2.0o at 90° C. These values confirm a similar relationship between fractionation magnitude and temperature that we found in laboratory-controlled silica-precipitation experiments. However, a relatively constant offset of ca. -2.9o between field and experimental fractionation values indicates that temperature alone cannot be responsible for the observed shifts. We infer that precipitation kinetics are a prominent control of silicon isotope fractionation in aqueous environments, whereby the influence of the extent of the system on the precipitation

Contribution of (222)Rn-bearing water to indoor radon and indoor air quality assessment in hot spring hotels of Guangdong, China.

Science.gov (United States)

Song, Gang; Wang, Xinming; Chen, Diyun; Chen, Yongheng

2011-04-01

This study investigates the contribution of radon ((222)Rn)-bearing water to indoor (222)Rn in thermal baths. The (222)Rn concentrations in air were monitored in the bathroom and the bedroom. Particulate matter (PM, both PM(10) and PM(2.5)) and carbon dioxide (CO(2)) were also monitored with portable analyzers. The bathrooms were supplied with hot spring water containing 66-260 kBq m(-3) of (222)Rn. The results show that the spray of hot spring water from the bath spouts is the dominant mechanism by which (222)Rn is released into the air of the bathroom, and then it diffuses into the bedroom. Average (222)Rn level was 110-410% higher in the bedrooms and 510-1200% higher in the bathrooms compared to the corresponding average levels when there was no use of hot spring water. The indoor (222)Rn levels were influenced by the (222)Rn concentrations in the hot spring water and the bathing times. The average (222)Rn transfer coefficients from water to air were 6.2 × 10(-4)-4.1 × 10(-3). The 24-h average levels of CO(2) and PM(10) in the hotel rooms were 89% and 22% higher than the present Indoor Air Quality (IAQ) standard of China. The main particle pollutant in the hotel rooms was PM(2.5). Radon and PM(10) levels in some hotel rooms were at much higher concentrations than guideline levels, and thus the potential health risks to tourists and especially to the hotel workers should be of great concern, and measures should be taken to lower inhalation exposure to these air pollutants. Copyright © 2011 Elsevier Ltd. All rights reserved.

Depositional facies and aqueous-solid geochemistry of travertine-depositing hot springs (Angel Terrace, Mammoth Hot Springs, Yellowstone National Park, USA)

Energy Technology Data Exchange (ETDEWEB)

Fouke, B.W.; Farmer, J.D.; Des Marais, D.J.; Pratt, L.; Sturchio, N.C.; Burns, P.C.; Discipulo, M.K.

2000-05-01

Petrographic and geochemical analyses of travertine-depositing hot springs at Angel Terrace, Mammoth Hot Springs, Yellowstone National Park, have been used to define five depositional facies along the spring drainage system. Spring waters are expelled in the vent facies at 71 to 73 C and precipitate mounded travertine composed of aragonite needle botryoids. The apron and channel facies (43--72 C) is floored by hollow tubes composed of aragonite needle botryoids that encrust sulfide-oxidizing Aquificales bacteria. The travertine of the pond facies (30--62 C) varies in composition from aragonite needle shrubs formed at higher temperatures to ridged networks of calcite and aragonite at lower temperatures. Calcite ice sheets, calcified bubbles, and aggregates of aragonite needles (fuzzy dumbbells) precipitate at the air-water interface and settle to pond floors. The proximal-slope facies (28--54 C), which forms the margins of terracette pools, is composed of arcuate aragonite needle shrubs that create small microterracettes on the steep slope face. Finally, the distal-slope facies (28--30 C) is composed of calcite spherules and calcite feather crystals. Despite the presence of abundant microbial mat communities and their observed role in providing substrates for mineralization, the compositions of spring-water and travertine predominantly reflect abiotic physical and chemical processes. Vigorous CO{sub 2} degassing causes a +2 unit increase in spring water pH, as well as Rayleigh-type covariations between the concentration of dissolved inorganic carbon and corresponding {delta}{sup 13}C. Travertine {delta}{sup 13}C and {delta}{sup 18}O are nearly equivalent to aragonite and calcite equilibrium values calculated from spring water in the higher-temperature ({approximately}50--73 C) depositional facies. Conversely, travertine precipitating in the lower-temperature (<{approximately}50 C) depositional facies exhibits {delta}{sup 13}C and {delta}{sup 18}O values that are as

Contribution of {sup 222}Rn-bearing water to indoor radon and indoor air quality assessment in hot spring hotels of Guangdong, China

Energy Technology Data Exchange (ETDEWEB)

Song Gang, E-mail: songg2005@126.co [School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006 (China); Wang Xinming [Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); Chen Diyun; Chen Yongheng [School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006 (China)

2011-04-15

This study investigates the contribution of radon ({sup 222}Rn)-bearing water to indoor {sup 222}Rn in thermal baths. The {sup 222}Rn concentrations in air were monitored in the bathroom and the bedroom. Particulate matter (PM, both PM{sub 10} and PM{sub 2.5}) and carbon dioxide (CO{sub 2}) were also monitored with portable analyzers. The bathrooms were supplied with hot spring water containing 66-260 kBq m{sup -3} of {sup 222}Rn. The results show that the spray of hot spring water from the bath spouts is the dominant mechanism by which {sup 222}Rn is released into the air of the bathroom, and then it diffuses into the bedroom. Average {sup 222}Rn level was 110-410% higher in the bedrooms and 510-1200% higher in the bathrooms compared to the corresponding average levels when there was no use of hot spring water. The indoor {sup 222}Rn levels were influenced by the {sup 222}Rn concentrations in the hot spring water and the bathing times. The average {sup 222}Rn transfer coefficients from water to air were 6.2 x 10{sup -4}-4.1 x 10{sup -3}. The 24-h average levels of CO{sub 2} and PM{sub 10} in the hotel rooms were 89% and 22% higher than the present Indoor Air Quality (IAQ) standard of China. The main particle pollutant in the hotel rooms was PM{sub 2.5}. Radon and PM{sub 10} levels in some hotel rooms were at much higher concentrations than guideline levels, and thus the potential health risks to tourists and especially to the hotel workers should be of great concern, and measures should be taken to lower inhalation exposure to these air pollutants. - Highlights: {yields} {sup 222}Rn-bearing water is the main contributor to indoor radon in hot spring hotel. {yields} The PM{sub 2.5} and CO{sub 2} are also the main indoor pollutants in the hotel rooms. {yields} Higher radon and PM levels might have significant negative health effects to human. {yields} The radon transfer coefficients are consistent with the published data.

Microbial ecology of two hot springs of Sikkim: Predominate population and geochemistry.

Science.gov (United States)

Najar, Ishfaq Nabi; Sherpa, Mingma Thundu; Das, Sayak; Das, Saurav; Thakur, Nagendra

2018-10-01

H, alkalinity, Ca 2+ , Mg 2+ , Cl 2+ , and sulfur were main environmental variables influencing the microbial community composition and diversity. Also the piper diagram suggested that the water of both the hot springs are Ca-HCO 3- type and can be predicted as shallow fresh ground waters. This study has provided an insight into the ecological interaction of the diverse microbial communities and associated physicochemical parameters, which will help in determining the future studies on different biogeochemical pathways in these hot springs. Copyright © 2018. Published by Elsevier B.V.

Metagenomic Study of Iron Homeostasis in Iron Depositing Hot Spring Cyanobacterial Community

Science.gov (United States)

Brown, I.; Franklin H.; Tringe, S. G.; Klatt, C. G.; Bryant, D. A.; Sarkisova, S. A.; Guevara, M.

2010-01-01

Introduction: It is not clear how an iron-rich thermal hydrosphere could be hospitable to cyanobacteria, since reduced iron appears to stimulate oxidative stress in all domains of life and particularly in oxygenic phototrophs. Therefore, metagenomic study of cyanobacterial community in iron-depositing hot springs may help elucidate how oxygenic prokaryotes can withstand the extremely high concentrations of reactive oxygen species (ROS) produced by interaction between environmental Fe2+ and O2. Method: Anchor proteins from various species of cyanobacteria and some anoxygenic phototrophs were selected on the basis of their hypothetical role in Fe homeostasis and the suppression of oxidative stress and were BLASTed against the metagenomes of iron-depositing Chocolate Pots and freshwater Mushroom hot springs. Results: BLASTing proteins hypothesized to be involved in Fe homeostasis against the microbiomes from the two springs revealed that iron-depositing hot spring has a greater abundance of defensive proteins such as bacterioferritin comigratory protein (Bcp) and DNA-binding Ferritin like protein (Dps) than a fresh-water hot spring. One may speculate that the abundance of Bcp and Dps in an iron-depositing hot spring is connected to the need to suppress oxidative stress in bacteria inhabiting environments with high Fe2+ concnetration. In both springs, Bcp and Dps are concentrated within the cyanobacterial fractions of the microbial community (regardless of abundance). Fe3+ siderophore transport (from the transport system permease protein query) may be less essential to the microbial community of CP because of the high [Fe]. Conclusion: Further research is needed to confirm that these proteins are unique to photoautotrophs such as those living in iron-depositing hot spring.

Enzyme activity screening of thermophilic bacteria isolated from Dusun Tua Hot Spring, Malaysia

Science.gov (United States)

Msarah, Marwan; Ibrahim, Izyanti; Aqma, Wan Syaidatul

2018-04-01

Thermophilic bacteria have biotechnological importance due to the availability of unique enzymes which are stable in extreme circumstances. The aim of this study includes to isolate thermophilic bacteria from hot spring and screen for important enzyme activities. Water samples from the Dusun Tua Hot Spring were collected and the physiochemical characterisation of water was measured. Eight thermophilic bacteria were isolated and determined to have at least three strong enzyme activity including protease, lipase, amylase, cellulase, pectinase and xylanase. The results showed that HuluC2 displayed all the enzyme activities and can be further studied.

Bacterial and archaeal diversities in Yunnan and Tibetan hot springs, China.

Science.gov (United States)

Song, Zhao-Qi; Wang, Feng-Ping; Zhi, Xiao-Yang; Chen, Jin-Quan; Zhou, En-Min; Liang, Feng; Xiao, Xiang; Tang, Shu-Kun; Jiang, Hong-Chen; Zhang, Chuanlun L; Dong, Hailiang; Li, Wen-Jun

2013-04-01

Thousands of hot springs are located in the north-eastern part of the Yunnan-Tibet geothermal zone, which is one of the most active geothermal areas in the world. However, a comprehensive and detailed understanding of microbial diversity in these hot springs is still lacking. In this study, bacterial and archaeal diversities were investigated in 16 hot springs (pH 3.2-8.6; temperature 47-96°C) in Yunnan Province and Tibet, China by using a barcoded 16S rRNA gene-pyrosequencing approach. Aquificae, Proteobacteria, Firmicutes, Deinococcus-Thermus and Bacteroidetes comprised the large portion of the bacterial communities in acidic hot springs. Non-acidic hot springs harboured more and variable bacterial phyla than acidic springs. Desulfurococcales and unclassified Crenarchaeota were the dominated groups in archaeal populations from most of the non-acidic hot springs; whereas, the archaeal community structure in acidic hot springs was simpler and characterized by Sulfolobales and Thermoplasmata. The phylogenetic analyses showed that Aquificae and Crenarchaeota were predominant in the investigated springs and possessed many phylogenetic lineages that have never been detected in other hot springs in the world. Thus findings from this study significantly improve our understanding of microbial diversity in terrestrial hot springs. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

Siliceous Shrubs in Yellowstone's Hot Springs: Implications for Exobiological Investigations

Science.gov (United States)

Guidry, S. A.; Chafetz, H. S.

2003-01-01

Potential relict hot springs have been identified on Mars and, using the Earth as an analog, Martian hot springs are postulated to be an optimal locality for recognizing preserved evidence of extraterrestrial life. Distinctive organic and inorganic biomarkers are necessary to recognize preserved evidence of life in terrestrial and extraterrestrial hot spring accumulations. Hot springs in Yellowstone National Park, Wyoming, U.S.A., contain a wealth of information about primitive microbial life and associated biosignatures that may be useful for future exobiological investigations. Numerous siliceous hot springs in Yellowstone contain abundant, centimeter-scale, spinose precipitates of opaline silica (opal-A). Although areally extensive in siliceous hot spring discharge channel facies, these spinose forms have largely escaped attention. These precipitates referred to as shrubs, consist of porous aggregates of spinose opaline silica that superficially resemble miniature woody plants, i.e., the term shrubs. Shrubs in carbonate precipitating systems have received considerable attention, and represent naturally occurring biotically induced precipitates. As such, shrubs have great potential as hot spring environmental indicators and, more importantly, proxies for pre-existing microbial life.

The Mycoflora of Hot Spring Soil in Northern Taiwan

Directory of Open Access Journals (Sweden)

Kuei-Yu Chen

2003-09-01

Full Text Available An investigation of the mycoflora in northern Taiwan from August 1999 to June 2000, particularly of thermophilic and thermotolerant fungi inhabiting sulfurous hot spring soils, resulted in identification 12 taxa: Aspergillus fumigatus var. fumigatus (66.85 %, A. fumigatus var. 1 with green colony (7.86 %, A. fumigatus var. 2 with brown colony (4.81 %, A. niger (1.14 %, unidentified Asperigillus sp. (0.045 %, Chrysosporium sp. (0.18 %, Papulaspora thermophila (2.72 %, Scytalidium thermophilum (0.045 %, Sporotrichum sp. (0.045 %, Mycelia sterilia sp.1 with white colony (6.63 %, Mycelia sterilia sp.2 with yellow colony (5.27 % and Mycelia sterilia sp. 3 with gray colony (4.405 %. A total of 2202 colonies were isolated from three sampling sites: site 1 (hot springhead, site 2 (2 m from site 1 and site 3 (4 m from site 1. Fungal colonies isolated as well as species percentage at three sites were as follows: 32.92 % in 9 taxa from site 1, 37.87 % in 11 taxa from site 2, and 29.21 % in 8 taxa from site 3. The dominant species was Aspergillus fumigatus var. fumigatus, which was isolated year around from three sampling sites. A. fumigatus var. 1 appeared from February to June 2000. A. fumigatus var. 2 was isolated only in August and October 1999. Within the sampling range of hot spring niches, there was evidence of the presence of ecotypes in the A. fumigatus complex. Chrysosporium sp. and Sporotrichum sp. were isolated only from the soils without hot water treatment, but Aspergillus sp. and Scytalidium thermophilum were isolated only from the soils pre-treated with hot water for 30 min. at 60℃. The significance level (P value of fungal communities between hot water treatment and no treatment was 0.866, indicating that no significant difference between both treatments.

Biodiversity of the microbial mat of the Garga hot spring.

Science.gov (United States)

Rozanov, Alexey Sergeevich; Bryanskaya, Alla Victorovna; Ivanisenko, Timofey Vladimirovich; Malup, Tatyana Konstantinovna; Peltek, Sergey Evgenievich

2017-12-28

Microbial mats are a good model system for ecological and evolutionary analysis of microbial communities. There are more than 20 alkaline hot springs on the banks of the Barguzin river inflows. Water temperature reaches 75 °C and pH is usually 8.0-9.0. The formation of microbial mats is observed in all hot springs. Microbial communities of hot springs of the Baikal rift zone are poorly studied. Garga is the biggest hot spring in this area. In this study, we investigated bacterial and archaeal diversity of the Garga hot spring (Baikal rift zone, Russia) using 16S rRNA metagenomic sequencing. We studied two types of microbial communities: (i) small white biofilms on rocks in the points with the highest temperature (75 °C) and (ii) continuous thick phototrophic microbial mats observed at temperatures below 70 °C. Archaea (mainly Crenarchaeota; 19.8% of the total sequences) were detected only in the small biofilms. The high abundance of Archaea in the sample from hot springs of the Baikal rift zone supplemented our knowledge of the distribution of Archaea. Most archaeal sequences had low similarity to known Archaea. In the microbial mats, primary products were formed by cyanobacteria of the genus Leptolyngbya. Heterotrophic microorganisms were mostly represented by Actinobacteria and Proteobacteria in all studied samples of the microbial mats. Planctomycetes, Chloroflexi, and Chlorobi were abundant in the middle layer of the microbial mats, while heterotrophic microorganisms represented mostly by Firmicutes (Clostridia, strict anaerobes) dominated in the bottom part. Besides prokaryotes, we detect some species of Algae with help of detection their chloroplasts 16 s rRNA. High abundance of Archaea in samples from hot springs of the Baikal rift zone supplemented our knowledge of the distribution of Archaea. Most archaeal sequences had low similarity to known Archaea. Metagenomic analysis of microbial communities of the microbial mat of Garga hot spring showed that

The source, discharge, and chemical characteristics of water from Agua Caliente Spring, Palm Springs, California

Science.gov (United States)

Contributors: Brandt, Justin; Catchings, Rufus D.; Christensen, Allen H.; Flint, Alan L.; Gandhok, Gini; Goldman, Mark R.; Halford, Keith J.; Langenheim, V.E.; Martin, Peter; Rymer, Michael J.; Schroeder, Roy A.; Smith, Gregory A.; Sneed, Michelle; Martin, Peter

2011-01-01

Agua Caliente Spring, in downtown Palm Springs, California, has been used for recreation and medicinal therapy for hundreds of years and currently (2008) is the source of hot water for the Spa Resort owned by the Agua Caliente Band of the Cahuilla Indians. The Agua Caliente Spring is located about 1,500 feet east of the eastern front of the San Jacinto Mountains on the southeast-sloping alluvial plain of the Coachella Valley. The objectives of this study were to (1) define the geologic structure associated with the Agua Caliente Spring; (2) define the source(s), and possibly the age(s), of water discharged by the spring; (3) ascertain the seasonal and longer-term variability of the natural discharge, water temperature, and chemical characteristics of the spring water; (4) evaluate whether water-level declines in the regional aquifer will influence the temperature of the spring discharge; and, (5) estimate the quantity of spring water that leaks out of the water-collector tank at the spring orifice.

Archaeal diversity in Icelandic hot springs

DEFF Research Database (Denmark)

Kvist, Thomas; Ahring, Birgitte Kiær; Westermann, Peter

2007-01-01

Whole-cell density gradient extractions from three solfataras (pH 2.5) ranging in temperature from 81 to 90 degrees C and one neutral hot spring (81 degrees C, pH 7) from the thermal active area of Hveragerethi (Iceland) were analysed for genetic diversity and local geographical variation...... of Archaea by analysis of amplified 16S rRNA genes. In addition to the three solfataras and the neutral hot spring, 10 soil samples in transects of the soil adjacent to the solfataras were analysed using terminal restriction fragment length polymorphism (t-RFLP). The sequence data from the clone libraries...... enzymes AluI and BsuRI. The sequenced clones from this solfatara belonged to Sulfolobales, Thermoproteales or were most closest related to sequences from uncultured Archaea. Sequences related to group I.1b were not found in the neutral hot spring or the hyperthermophilic solfatara (90 degrees C)....

Evaluation of radon in hot spring waters in Zacatecas State, Mexico

International Nuclear Information System (INIS)

Favila R, E.; Lopez del Rio, H.; Davila R, I.; Mireles G, F.

2010-10-01

It is well know that radon is a potent human carcinogen. Because of the health concern of radon exposure, concentrations of 222 Rn were determined in ten hot spring water samples from the Mexican state of Zacatecas. The thermal water is collected in pools and used mainly for recreational purposes. In addition to radon level, the water samples were characterized for temperature, conductivity, and ph. Liquid scintillation spectrometry was used to measure 222 Rn and its decay products by mixing directly an aliquot of water with a commercial liquid scintillation. All measurements were carried out using a liquid scintillation counter (Wallac 1411). The water temperature ranged from 28 to 59 C, while the ph varied from 7.2 to 9.0, and the water conductivity was between 202.4 and 1072 μS/cm. The 222 Rn concentration varied in the range 3.9-32.6 Bq/L. In addition, the risk to radon exposure was assessed by considering three -real and possible- radon exposure scenarios: 1) ingestion of bottled thermal water, 2) direct ingestion of thermal water; and 3) vapor inhalation. The annual effective dose calculated for ingestion of bottled thermal water was 0.010-0.083 mSv/yr; for ingestion of water was 0.65-5.47 mSv/yr; and for inhalation was 0.28-2.81 mSv/yr. (Author)

Microbiological studies of hot springs in India: a review.

Science.gov (United States)

Poddar, Abhijit; Das, Subrata K

2018-01-01

The earliest microbiological studies on hot springs in India date from 2003, a much later date compared to global attention in this striking field of study. As of today, 28 out of 400 geothermal springs have been explored following both culturable and non-culturable approaches. The temperatures and pH of the springs are 37-99 °C and 6.8-10, respectively. Several studies have been performed on the description of novel genera and species, characterization of different bio-resources, metagenomics of hot spring microbiome and whole genome analysis of few isolates. 17 strains representing novel species and many thermostable enzymes, including lipase, protease, chitinase, amylase, etc. with potential biotechnological applications have been reported by several authors. Influence of physico-chemical conditions, especially that of temperature, on shaping the hot spring microbiome has been established by metagenomic investigations. Bacteria are the predominant life forms in all the springs with an abundance of phyla Firmicutes, Proteobacteria, Actinobacteria, Thermi, Bacteroidetes, Deinococcus-Thermus and Chloroflexi. In this review, we have discussed the findings on all microbiological studies that have been carried out to date, on the 28 hot springs. Further, the possibilities of extrapolating these studies for practical applications and environmental impact assessment towards protection of natural ecosystem of hot springs have also been discussed.

Distribution of glycerol dialkyl glycerol tetraethers in Tibetan hot springs

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Liu He

2012-05-01

Full Text Available Isoprenoidal glycerol dialkyl glycerol tetraethers (iGDGTs from the Gulu hot springs (23–83.6 °C, pH > 7 and Yangbajing hot springs (80–128 °C, pH > 7 were analyzed in order to investigate the distribution of archaeal lipids among different hot springs in Tibet. A soil sample from Gulu was incubated at different temperatures and analyzed for changes in iGDGTs to help evaluate whether surrounding soil may contribute to the iGDGTs in hot springs. The sources of bacterial GDGTs (bGDGTs in these hot springs were also investigated. The results revealed different profiles of iGDGTs between Gulu and Yangbajing hot springs. Core iGDGTs and polar iGDGTs also presented different patterns in each hot spring. The PCA analysis showed that the structure of polar iGDGTs can be explained by three factors and suggested multiple sources of these compounds. Bivariate correlation analysis showed significant positive correlations between polar and core bGDGTs, suggesting the in situ production of bGDGTs in the hot springs. Furthermore, in the soil incubation experiment, temperature had the most significant influence on concentration of bGDGTs rather than iGDGTs, and polar bGDGTs had greater variability than core bGDGTs with changing temperature. Our results indicated that soil input had little influence on the composition of GDGTs in Tibetan hot springs. On the other hand, ring index and TEX86 values were both positively correlated with incubation temperature, suggesting that the structure of archaeal lipids changed in response to varying temperature during incubation.

Diversity and Distribution of Thermophilic Bacteria in Hot Springs of Pakistan.

Science.gov (United States)

Amin, Arshia; Ahmed, Iftikhar; Salam, Nimaichand; Kim, Byung-Yong; Singh, Dharmesh; Zhi, Xiao-Yang; Xiao, Min; Li, Wen-Jun

2017-07-01

Chilas and Hunza areas, located in the Main Mantle Thrust and Main Karakoram Thrust of the Himalayas, host a range of geochemically diverse hot springs. This Himalayan geothermal region encompassed hot springs ranging in temperature from 60 to 95 °C, in pH from 6.2 to 9.4, and in mineralogy from bicarbonates (Tato Field), sulfates (Tatta Pani) to mixed type (Murtazaabad). Microbial community structures in these geothermal springs remained largely unexplored to date. In this study, we report a comprehensive, culture-independent survey of microbial communities in nine samples from these geothermal fields by employing a bar-coded pyrosequencing technique. The bacterial phyla Proteobacteria and Chloroflexi were dominant in all samples from Tato Field, Tatta Pani, and Murtazaabad. The community structures however depended on temperature, pH, and physicochemical parameters of the geothermal sites. The Murtazaabad hot springs with relatively higher temperature (90-95 °C) favored the growth of phylum Thermotogae, whereas the Tatta Pani thermal spring site TP-H3-b (60 °C) favored the phylum Proteobacteria. At sites with low silica and high temperature, OTUs belonging to phylum Chloroflexi were dominant. Deep water areas of the Murtazaabad hot springs favored the sulfur-reducing bacteria. About 40% of the total OTUs obtained from these samples were unclassified or uncharacterized, suggesting the presence of many undiscovered and unexplored microbiota. This study has provided novel insights into the nature of ecological interactions among important taxa in these communities, which in turn will help in determining future study courses in these sites.

Hydrogeochemical Characteristics and Evolution of Hot Springs in Eastern Tibetan Plateau Geothermal Belt, Western China: Insight from Multivariate Statistical Analysis

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Zheming Shi

2017-01-01

Full Text Available The eastern Tibetan Plateau geothermal belt is one of the important medium-high temperature geothermal belts in China. However, less work has been done on the hydrochemical characteristic and its geological origin. Understanding the chemical characteristics and the hydrochemical evolution processes is important in evaluating the geothermal energy potential in this area. In the present study, we discussed the hydrochemical properties and their origins of 39 hot springs located in the eastern Tibetan Plateau geothermal belt (Kangding-Litang-Batang geothermal belt. Cluster analysis and factor analysis are employed to character the hydrochemical properties of hot springs in different fault zones and the possible hydrochemical evolution processes of these hot springs. Our study shows that the hot springs can be divided into three groups based on their locations. The hot springs in the first group mainly originate from the volcanic rock and the springs in the second group originate from the metamorphic rock while the springs in the third group originate from the result of mixture of shallow water. Water-rock interaction, cation exchange, and the water environment are the three dominant factors that control the hydrochemical evolution process in the eastern Tibetan Plateau. These results are also in well agreement with the isotopic and chemical analysis.

Legionella thermalis sp. nov., isolated from hot spring water in Tokyo, Japan.

Science.gov (United States)

Ishizaki, Naoto; Sogawa, Kazuyuki; Inoue, Hiroaki; Agata, Kunio; Edagawa, Akiko; Miyamoto, Hiroshi; Fukuyama, Masafumi; Furuhata, Katsunori

2016-03-01

Strain L-47(T) of a novel bacterial species belonging to the genus Legionella was isolated from a sample of hot spring water from Tokyo, Japan. The 16S rRNA gene sequences (1477 bp) of this strain (accession number AB899895) had less than 95.0% identity with other Legionella species. The dominant fatty acids of strain L-47(T) were a15:0 (29.6%) and the major ubiquinone was Q-12 (71.1%). It had a guanine-plus-cytosine content of 41.5 mol%. The taxonomic description of Legionella thermalis sp. nov. is proposed to be type strain L-47(T) (JCM 30970(T)  = KCTC 42799(T)). © 2016 The Societies and John Wiley & Sons Australia, Ltd.

Study of tourists exposure rate in Mahallat hot Spring Region

Energy Technology Data Exchange (ETDEWEB)

Tavakoli, H. M.B. [Isfahan Univ. of Medical Sciences, Isfahan (Iran, Islamic Republic of); Fallah, M.G. [Isfahan University of Medical Sciences (Iran, Islamic Republic of); Ghiasinejad, M. [Iran Atomic Energy Organization, Tehran (Iran, Islamic Republic of)

2006-07-01

Introduction: High level radiation areas have been recognized on various parts of the earth. Some of these areas include: Brasilia, India, and Iran. Mahallat hot spring region in the central part of Iran is also one of these areas. Study of exposure in these areas could be helpful in investigating the effects of ionizing radiation. Materials and Methods: In addition to several seasonal springs, Mahallat hot spring region contains five permanent springs named: Soleimani, Shafa, Dombe, Romatism and Sauda. Internal exposure (due to inhalation of radon gas and drinking water) and external exposure (due to cosmic rays and radioactive elements in the ground) to the tourists was studied. Used materials and apparatus include: RSS -112 ionizing chamber for environmental gamma rays exposure measurement, highly pure germanium detector for measuring radioactive elements in the ground, liquid scintillation counter for measuring {sup 222}Rn gas concentration in water samples, Bubbler chamber and Locus cells for Rn concentration measurements (Emanation method) and Alfa guard detector for {sup 226}Ra concentration measurements. Conclusions and Discussion: A total of 270 visitors are included in this study. Considering residual durations of the studied group in open and closed environment of bathrooms, hotel and inn rooms, obtained annual external effective dose is 75.4{+-}8.7{mu}Sv and 138.3{+-}11.8{mu}Sv for natives and travelers respectively. EEC coefficients has been used for calculating annual internal effective dose due to radon gas inhalation. Annual internal effective dose, in this path, is 0.9 and 2.1 mSv in open and closed environment for native and visitors respectively. Annual internal effective dose due to drinking water, is 0.43 and 0.09{mu}Sv for natives people and travelers, respectively. Measurements show that more than 90% of the received dose in the studied groups is due to radon gas inhalation. External and internal dose summation is 0.98 mSv for natives and 2

Study of tourists exposure rate in Mahallat hot Spring Region

International Nuclear Information System (INIS)

Tavakoli, H. M.B.; Fallah, M.G.; Ghiasinejad, M.

2006-01-01

Introduction: High level radiation areas have been recognized on various parts of the earth. Some of these areas include: Brasilia, India, and Iran. Mahallat hot spring region in the central part of Iran is also one of these areas. Study of exposure in these areas could be helpful in investigating the effects of ionizing radiation. Materials and Methods: In addition to several seasonal springs, Mahallat hot spring region contains five permanent springs named: Soleimani, Shafa, Dombe, Romatism and Sauda. Internal exposure (due to inhalation of radon gas and drinking water) and external exposure (due to cosmic rays and radioactive elements in the ground) to the tourists was studied. Used materials and apparatus include: RSS -112 ionizing chamber for environmental gamma rays exposure measurement, highly pure germanium detector for measuring radioactive elements in the ground, liquid scintillation counter for measuring 222 Rn gas concentration in water samples, Bubbler chamber and Locus cells for Rn concentration measurements (Emanation method) and Alfa guard detector for 226 Ra concentration measurements. Conclusions and Discussion: A total of 270 visitors are included in this study. Considering residual durations of the studied group in open and closed environment of bathrooms, hotel and inn rooms, obtained annual external effective dose is 75.4±8.7μSv and 138.3±11.8μSv for natives and travelers respectively. EEC coefficients has been used for calculating annual internal effective dose due to radon gas inhalation. Annual internal effective dose, in this path, is 0.9 and 2.1 mSv in open and closed environment for native and visitors respectively. Annual internal effective dose due to drinking water, is 0.43 and 0.09μSv for natives people and travelers, respectively. Measurements show that more than 90% of the received dose in the studied groups is due to radon gas inhalation. External and internal dose summation is 0.98 mSv for natives and 2.2 mSv for for

Amorphous calcium carbonate associated with biofilms in hot spring deposits

Science.gov (United States)

Jones, Brian; Peng, Xiaotong

2012-08-01

Calcium carbonate nanoparticles are intimately associated with crystalline calcite and aragonite in the Eryuan, Gongxiaoshe, and Zhuyuan hot springs (water temperature > 75 °C), which are located in Yunnan Province, China. The nanoparticles, springs, the ACC is always found under, in, or on top of biofilms, commonly in close proximity to crystalline calcite and/or aragonite. Textural evidence indicates that the ACC probably developed in microdomains that develop in the complex biofilm hydrogels. Critically, there is no evidence to support the notion that the nanoparticles are calcified nannobacteria. In the Chinese springs, ACC appears to play a formative role in the development of wheat-sheaf arrays of aragonite crystals and some of the calcite crystals. Hollow cores in some of the aragonite bundles probably formed as ACC was dissolved and many of the aragonite crystals appear to have developed as ACC recrystallized. Similarly, layers of ACC that coat the surfaces of some calcite crystals could be diagenetically transformed into calcite. The development of ACC in hot spring systems may be widespread and may play a critical but transitory role in the development of crystalline CaCO3 in these high temperature environments.

Comparison of the microbial communities of hot springs waters and the microbial biofilms in the acidic geothermal area of Copahue (Neuquén, Argentina).

Science.gov (United States)

Urbieta, María Sofía; González-Toril, Elena; Bazán, Ángeles Aguilera; Giaveno, María Alejandra; Donati, Edgardo

2015-03-01

Copahue is a natural geothermal field (Neuquén province, Argentina) dominated by the Copahue volcano. As a consequence of the sustained volcanic activity, Copahue presents many acidic pools, hot springs and solfataras with different temperature and pH conditions that influence their microbial diversity. The occurrence of microbial biofilms was observed on the surrounding rocks and the borders of the ponds, where water movements and thermal activity are less intense. Microbial biofilms are particular ecological niches within geothermal environments; they present different geochemical conditions from that found in the water of the ponds and hot springs which is reflected in different microbial community structure. The aim of this study is to compare microbial community diversity in the water of ponds and hot springs and in microbial biofilms in the Copahue geothermal field, with particular emphasis on Cyanobacteria and other photosynthetic species that have not been detected before in Copahue. In this study, we report the presence of Cyanobacteria, Chloroflexi and chloroplasts of eukaryotes in the microbial biofilms not detected in the water of the ponds. On the other hand, acidophilic bacteria, the predominant species in the water of moderate temperature ponds, are almost absent in the microbial biofilms in spite of having in some cases similar temperature conditions. Species affiliated with Sulfolobales in the Archaea domain are the predominant microorganism in high temperature ponds and were also detected in the microbial biofilms.

Microstructure of Sinter Deposit Formed at Hot Springs in West Sumatera

Science.gov (United States)

Putra, A.; Inanda, D. Y.; Buspa, F.; Salim, A. F.

2018-03-01

Sinter deposit emerged and spread at several hot springs in West Sumatera is divided into three types, they are full silica, half silica-carbonate and full carbonate. This work intends to investigate the characteristic of each type by its crystalline structure and morphology and its correlation to surface temperature. The research is focused on Sapan Maluluang hot spring (full silica), Garara hot spring (half silica-carbonate) and Bawah Kubang hot spring (full carbonate). Crystalline structure is analyzed by X-Ray Diffraction (XRD) methods, it showed that deposit from Sapan Maluluang has opal-A structure, Garara has opal-CT structure and Bawah Kubang has crystalline structure. The Scanning Electron Microscopy (SEM) methods is applied to describe its morphology surface, in which spherical, almost rounded and irregular textured was formed at each deposit, respectively. Surface temperature of hot spring also has given impact on deposit texture.

Multi proxy approach to evaluate and delineate the potential of hot springs in the Kotli District (Kashmir, Pakistan)

Energy Technology Data Exchange (ETDEWEB)

Anees, M.; Shah, M.; Qureshi, A.; Manzoor, S.

2017-11-01

Tattapani hot springs are located near the Kotli District of Azad Kashmir, Pakistan. This study evaluates these hot springs based on surface geological information, radon emission measurements, hydro-geochemical and isotopic signatures and potential source mechanisms. Field observations reveal that the hot springs are located at the crest of the Tattapani anticline along the faulted contact of Cambrian carbonates with Paleocene siliciclastics. In addition, remnants of igneous intrusions in the Cambrian carbonates are commonly observed. Spatial distribution of radon emissions (ranging between 2.1 and 29.5KBq m-3) indicates an anomalous zone located over the Cambrian-Paleocene faulted contact. Hydro-geochemical data show sodium-bicarbonate affinity of hot springs. The highest surface temperature of these springs is recorded at 60.8ºC. Average reservoir temperatures based on silica and cation geo-thermometers are 101ºC and 115ºC, respectively. Giggenbach ternary diagram (Na-K-Mg) suggests a non-equilibrium state between fluid and rock, whereas isotopic and chemical data indicate heat loss by conductive cooling and mixing with groundwater during the flow of thermal water up to the surface. Oxygen and deuterium isotopes indicate that thermal water is of meteoric origin, rain and/or snow in the north at higher altitudes providing the potential recharge. Furthermore, absence of tritium in the thermal water suggests a residence time of more than 50 years.

Biogeochemical characteristics of Kuan-Tzu-Ling, Chung-Lun and Bao-Lai hot springs in southern Taiwan.

Science.gov (United States)

Maity, Jyoti Prakash; Liu, Chia-Chuan; Nath, Bibhash; Bundschuh, Jochen; Kar, Sandeep; Jean, Jiin-Shuh; Bhattacharya, Prosun; Liu, Jiann-Hong; Atla, Shashi B; Chen, Chien-Yen

2011-01-01

Hot springs are the important natural sources of geothermally heated groundwater from the Earth's crust. Kuan-Tzu-Ling (KTL), Chung-Lun (CL) and Bao-Lai (BL) are well-known hot springs in southern Taiwan. Fluid and mud (sediments) samples were collected from the eruption points of three hot springs for detailed biogeochemical characterization. The fluid sample displays relatively high concentrations of Na(+) and Cl(-) compared with K(+), Mg(2+), Ca(2+), NO(2) (-), and SO(4) (2-), suggesting a possible marine origin. The concentrations of Fe, Cr, Mn, Ni, V and Zn were significantly higher in the mud sediments compared with fluids, whereas high concentrations of As, Ba, Cu, Se, Sr and Rb were observed in the fluids. This suggests that electronegative elements were released during sediment-water interactions. High As concentration in the fluids was observed to be associated with low redox (Eh) conditions. The FTIR spectra of the humic acid fractions of the sediments showed the presence of possible functional groups of secondary amines, ureas, urethanesm (amide), and silicon. The sulfate-reducing deltaproteobacterium 99% similar to Desulfovibrio psychrotolerans (GU329907) were rich in the CL hot spring while mesophilic, proteolytic, thiosulfate- and sulfur-reducing bacterium that 99% similar to Clostridium sulfidigenes (GU329908) were rich in the BL hot spring.

Determining barriers to developing geothermal power generation in Japan: Societal acceptance by stakeholders involved in hot springs

International Nuclear Information System (INIS)

Kubota, Hiromi; Hondo, Hiroki; Hienuki, Shunichi; Kaieda, Hideshi

2013-01-01

After many years of stagnant growth in geothermal power generation, development plans for new geothermal plants have recently emerged throughout Japan. Through a literature review, we investigated the relationships between the principal barriers to geothermal development and we thereby analyzed the deciding factors in the future success of such enterprises. The results show that the societal acceptance of geothermal power by local stakeholders is the fundamental barrier as it affects almost all other barriers, such as financial, technical, and political risks. Thus, we conducted semi-structured interviews with 26 stakeholders including developers, hot spring inn managers, and local government officials. Some hot spring inn managers and local government officials noted that they have always been strongly concerned about the adverse effects of geothermal power generation on hot springs; their opposition has delayed decision-making by local governments regarding drilling permits, prolonged lead times, and caused other difficulties. A key reason for opposition was identified as uncertainty about the reversibility and predictability of the adverse effects on hot springs and other underground structures by geothermal power production and reinjection of hot water from reservoirs. Therefore, we discuss and recommend options for improving the risk management of hot springs near geothermal power plants. - Highlights: • We clarify relationships between barriers to geothermal power development in Japan. • Local acceptance by hot spring managers is the most prominent barrier. • Uncertainty of reversibility and predictability induces low acceptance. • Risk transfer system and dialogue are needed to alleviate concerns

Geologic setting and chemical characteristics of hot springs in central and western Alaska

Science.gov (United States)

Miller, Thomas P.; Barnes, Ivan; Pattan, William Wallace

1973-01-01

Numerous hot springs occur in a variety of geologic provinces in central and western Alaska. Granitic plutons are common to all the provinces and the hot springs are spatially associated with the contacts of these plutons. Of 23 hot springs whose bedrock geology is known, all occur within 3 miles of a granitic pluton. The occurrence of hot springs, however, appears to be independent of the age, composition, or magmatic history of the pluton.

Sol Duc Hot Springs feasibility study

Energy Technology Data Exchange (ETDEWEB)

1981-12-01

Sol Duc Springs is located in the Olympic National Park in western Washington state. Since the turn of the century, the area has served as a resort, offering hot mineral baths, lodge and overnight cabin accommodations. The Park Service, in conjunction with the concessionaire, is in the process of renovating the existing facilities, most of which are approximately 50 years old. The present renovation work consists of removing all of the existing cabins and replacing them with 36 new units. In addition, a new hot pool is planned to replace the existing one. This report explores the possibility of a more efficient use of the geothermal resource to accompany other planned improvements. It is important to note that the system outlined is based upon the resource development as it exists currently. That is, the geothermal source is considered to be: the two existing wells and the hot springs currently in use. In addition, every effort has been made to accommodate the priorities for utilization as set forth by the Park Service.

Morphological and phylogenetic diversity of thermophilic cyanobacteria in Algerian hot springs.

Science.gov (United States)

Amarouche-Yala, Samia; Benouadah, Ali; El Ouahab Bentabet, Abd; López-García, Purificación

2014-11-01

Geothermal springs in Algeria have been known since the Roman Empire. They mainly locate in Eastern Algeria and are inhabited by thermophilic organisms, which include cyanobacteria forming mats and concretions. In this work, we have investigated the cyanobacterial diversity of these springs. Cyanobacteria were collected from water, concretions and mats in nine hot springs with water temperatures ranging from 39 to 93 °C. Samples were collected for isolation in culture, microscopic morphological examination, and molecular diversity analysis based on 16S rRNA gene sequences. Nineteen different cyanobacterial morphotypes were identified, the most abundant of which were three species of Leptolyngbya, accompanied by members of the genera Gloeocapsa, Gloeocapsopsis, Stigonema, Fischerella, Synechocystis, Microcoleus, Cyanobacterium, Chroococcus and Geitlerinema. Molecular diversity analyses were in good general agreement with classical identification and allowed the detection of additional species in three springs with temperatures higher than 50 °C. They corresponded to a Synechococcus clade and to relatives of the intracellularly calcifying Candidatus Gloeomargarita lithophora. The hottest springs were dominated by members of Leptolyngbya, Synechococcus-like cyanobacteria and Gloeomargarita, whereas Oscillatoriales other than Leptolyngbya, Chroococcales and Stigonematales dominated lower temperature springs. The isolation of some of these strains sets the ground for future studies on the biology of thermophilic cyanobacteria.

Beneficial effect of hot spring bathing on stress levels in Japanese macaques.

Science.gov (United States)

Takeshita, Rafaela S C; Bercovitch, Fred B; Kinoshita, Kodzue; Huffman, Michael A

2018-05-01

The ability of animals to survive dramatic climates depends on their physiology, morphology and behaviour, but is often influenced by the configuration of their habitat. Along with autonomic responses, thermoregulatory behaviours, including postural adjustments, social aggregation, and use of trees for shelter, help individuals maintain homeostasis across climate variations. Japanese macaques (Macaca fuscata) are the world's most northerly species of nonhuman primates and have adapted to extremely cold environments. Given that thermoregulatory stress can increase glucocorticoid concentrations in primates, we hypothesized that by using an available hot spring, Japanese macaques could gain protection against weather-induced cold stress during winter. We studied 12 adult female Japanese macaques living in Jigokudani Monkey Park, Japan, during the spring birth season (April to June) and winter mating season (October to December). We collected faecal samples for determination of faecal glucocorticoid (fGC) metabolite concentrations by enzyme immunoassay, as well as behavioural data to determine time spent in the hot springs, dominance rank, aggression rates, and affiliative behaviours. We used nonparametric statistics to examine seasonal changes in hot spring bathing, and the relationship between rank and air temperature on hot spring bathing. We used general linear mixed-effect models to examine factors impacting hormone concentrations. We found that Japanese macaques use hot spring bathing for thermoregulation during the winter. In the studied troop, the single hot spring is a restricted resource favoured by dominant females. High social rank had both costs and benefits: dominant females sustained high fGC levels, which were associated with high aggression rates in winter, but benefited by priority of access to the hot spring, which was associated with low fGC concentrations and therefore might help reduce energy expenditure and subsequent body heat loss. This unique

Effects of Physiochemical Factors on Prokaryotic Biodiversity in Malaysian Circumneutral Hot Springs

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Chia S. Chan

2017-07-01

Full Text Available Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US, Sungai Klah (SK, Dusun Tua (DT, Sungai Serai (SS, Semenyih (SE, and Ayer Hangat (AH hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3–V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334–26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.

Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia

Directory of Open Access Journals (Sweden)

Heni Yohandini

2015-07-01

Full Text Available A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80 °C–91 °C and pH 7–8. We used a simple method for culturing the microbes, by enriching the spring water with nutrient broth media. Phylogenetic analysis showed that the method could recover microbes, which clustered within four distinct taxonomic groups: Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. These microbes closely related to Anoxybacillus rupiensis, Anoxybacillus flavithermus, Geobacillus pallidus, Brevibacillus thermoruber, Bacillus licheniformis, and Bacillus thermoamylovorans. The 16S ribosomal RNA gene sequence of one isolate only had 96% similarity with Brevibacillus sequence in GenBank.

Feasibility for development of an aquaculture facility at Hot Spring Cove

Energy Technology Data Exchange (ETDEWEB)

1986-01-01

This report describes the feasibilty of obtaining geothermally warmed water for use in aquaculture at Hot Springs Cove, British Columbia, and concludes that while the sources can probably be assessed from two sites in the cove, neither this nor the quantity of water available can be known for certain without field trials. The report also examines the feasibility of culturing various species of sea life at Hot Springs Cove, and concludes that a combination of rearing coho salmon smolts and oysters, with the late addition of tilapia, appears to be the most suitable both for biological and economic reasons. The total capital investment amounts to about $1,033,000. Operating costs would be about $450,000 annually, and additional capital to cover this would be needed in the first years of operation. A business plan is provided which includes cash flow projections for the first nine years of operation, and this shows that a maximum investment of approximately $1.2 million would be needed by the third year of operation. If sufficient warm water is available, and the facility is operated successfully, it should pay off the investment in seven to nine years, provided that interest free loans are available for capital investments. 20 refs., 1 fig., 8 tabs.

Comparative Metagenomics of Eight Geographically Remote Terrestrial Hot Springs.

Science.gov (United States)

Menzel, Peter; Gudbergsdóttir, Sóley Ruth; Rike, Anne Gunn; Lin, Lianbing; Zhang, Qi; Contursi, Patrizia; Moracci, Marco; Kristjansson, Jakob K; Bolduc, Benjamin; Gavrilov, Sergey; Ravin, Nikolai; Mardanov, Andrey; Bonch-Osmolovskaya, Elizaveta; Young, Mark; Krogh, Anders; Peng, Xu

2015-08-01

Hot springs are natural habitats for thermophilic Archaea and Bacteria. In this paper, we present the metagenomic analysis of eight globally distributed terrestrial hot springs from China, Iceland, Italy, Russia, and the USA with a temperature range between 61 and 92 (∘)C and pH between 1.8 and 7. A comparison of the biodiversity and community composition generally showed a decrease in biodiversity with increasing temperature and decreasing pH. Another important factor shaping microbial diversity of the studied sites was the abundance of organic substrates. Several species of the Crenarchaeal order Thermoprotei were detected, whereas no single bacterial species was found in all samples, suggesting a better adaptation of certain archaeal species to different thermophilic environments. Two hot springs show high abundance of Acidithiobacillus, supporting the idea of a true thermophilic Acidithiobacillus species that can thrive in hyperthermophilic environments. Depending on the sample, up to 58 % of sequencing reads could not be assigned to a known phylum, reinforcing the fact that a large number of microorganisms in nature, including those thriving in hot environments remain to be isolated and characterized.

Subaqueous hot springs in Köyceğiz Lake, Dalyan Channel and Fethiye-Göcek Bay (SW Turkey): Locations, chemistry and origins

KAUST Repository

Avşar, Özgür

2017-08-07

In this study, horizontal temperature measurements along organized grids have been used to detect subaqueous hot springs. The study area, located in the southwest of Turkey and comprised of Köyceğiz Lake, Dalyan Channel and Fethiye-Göcek Bay, was scanned by measuring temperatures horizontally, 2–3m above the bottom of the lake or sea. After analyzing the temperature data along the grids, the locations with anomalous temperature values were detected, and divers headed here for further verification. Accordingly, among these anomalies, the divers confirmed seven of them as subaqueous hot springs. Three of these hot springs are located in the Köyceğiz Lake, three of them are located in the Dalyan Channel and one hot spring is located in the Fethiye-Göcek Bay. At the locations where temperature anomalies were detected, the divers collected samples directly from the subaqueous hot spring using a syringe-type sampler. We evaluated these water samples together with samples collected from hot and cold springs on land and from local rivers, lakes and the sea, with an aim to generate a conceptual hydrogeochemical model of the geothermal system in the study area. This model predicts that rainwater precipitating in the highlands percolates through fractures and faults into the deeper parts of the Earth\\'s crust, here it is heated and ascends through the sea bottom via buried faults. Pervious carbonate nappes that are underlain and overlain by impervious rocks create a confined aquifer. The southern boundary of the Carbonate-Marmaris nappes is buried under alluvium and/or sea/lake water bodies and this phenomenon determines whether hot springs occur on land or subaqueous. The chemical and isotopic properties of the hot springs point to seawater mixing at deep levels. Thus, the mixing most probably occurs while the water is ascending through the faults and fractures. The gas geochemistry results reveal that the lowest mantle He contributions occur in the samples from K�

Subaqueous hot springs in Köyceğiz Lake, Dalyan Channel and Fethiye-Göcek Bay (SW Turkey): Locations, chemistry and origins

Science.gov (United States)

Avşar, Özgür; Avşar, Ulaş; Arslan, Şebnem; Kurtuluş, Bedri; Niedermann, Samuel; Güleç, Nilgün

2017-10-01

In this study, horizontal temperature measurements along organized grids have been used to detect subaqueous hot springs. The study area, located in the southwest of Turkey and comprised of Köyceğiz Lake, Dalyan Channel and Fethiye-Göcek Bay, was scanned by measuring temperatures horizontally, 2-3 m above the bottom of the lake or sea. After analyzing the temperature data along the grids, the locations with anomalous temperature values were detected, and divers headed here for further verification. Accordingly, among these anomalies, the divers confirmed seven of them as subaqueous hot springs. Three of these hot springs are located in the Köyceğiz Lake, three of them are located in the Dalyan Channel and one hot spring is located in the Fethiye-Göcek Bay. At the locations where temperature anomalies were detected, the divers collected samples directly from the subaqueous hot spring using a syringe-type sampler. We evaluated these water samples together with samples collected from hot and cold springs on land and from local rivers, lakes and the sea, with an aim to generate a conceptual hydrogeochemical model of the geothermal system in the study area. This model predicts that rainwater precipitating in the highlands percolates through fractures and faults into the deeper parts of the Earth's crust, here it is heated and ascends through the sea bottom via buried faults. Pervious carbonate nappes that are underlain and overlain by impervious rocks create a confined aquifer. The southern boundary of the Carbonate-Marmaris nappes is buried under alluvium and/or sea/lake water bodies and this phenomenon determines whether hot springs occur on land or subaqueous. The chemical and isotopic properties of the hot springs point to seawater mixing at deep levels. Thus, the mixing most probably occurs while the water is ascending through the faults and fractures. The gas geochemistry results reveal that the lowest mantle He contributions occur in the samples from K�

An environmental survey of Serpentine Hot Springs: Geology, hydrology, geochemistry, and microbiology

Science.gov (United States)

Nordstrom, D. Kirk; Hasselbach, Linda; Ingebritsen, Steven E.; Skorupa, Dana; McCleskey, R. Blaine; McDermott, Timothy R.

2015-01-01

Serpentine Hot Springs is the most visited site in the Bering Land Bridge National Preserve. The hot springs have traditionally been used by the Native people of the Seward Peninsula for religious, medicinal and spiritual purposes and continue to be used in many of the same ways by Native people today. The hot springs are also popular with non-Native users from Nome and other communities, recreational users and pilots from out of the area, and hunters and hikers.

Hyperspatial Thermal Imaging of Surface Hydrothermal Features at Pilgrim Hot Springs, Alaska using a small Unmanned Aerial System (sUAS)

Science.gov (United States)

Haselwimmer, C. E.; Wilson, R.; Upton, C.; Prakash, A.; Holdmann, G.; Walker, G.

2013-12-01

Thermal remote sensing provides a valuable tool for mapping and monitoring surface hydrothermal features associated with geothermal activity. The increasing availability of low-cost, small Unmanned Aerial Systems (sUAS) with integrated thermal imaging sensors offers a means to undertake very high spatial resolution (hyperspatial), quantitative thermal remote sensing of surface geothermal features in support of exploration and long-term monitoring efforts. Results from the deployment of a quadcopter sUAS equipped with a thermal camera over Pilgrim Hot Springs, Alaska for detailed mapping and heat flux estimation for hot springs, seeps, and thermal pools are presented. Hyperspatial thermal infrared imagery (4 cm pixels) was acquired over Pilgrim Hot Springs in July 2013 using a FLIR TAU 640 camera operating from an Aeryon Scout sUAS flying at an altitude of 40m. The registered and mosaicked thermal imagery is calibrated to surface temperature values using in-situ measurements of uniform blackbody tarps and the temperatures of geothermal and other surface pools acquired with a series of water temperature loggers. Interpretation of the pre-processed thermal imagery enables the delineation of hot springs, the extents of thermal pools, and the flow and mixing of individual geothermal outflow plumes with an unprecedented level of detail. Using the surface temperatures of thermal waters derived from the FLIR data and measured in-situ meteorological parameters the hot spring heat flux and outflow rate is calculated using a heat budget model for a subset of the thermal drainage. The heat flux/outflow rate estimates derived from the FLIR data are compared against in-situ measurements of the hot spring outflow rate recorded at the time of the thermal survey.

Radiation education using local environment. Educational experiment using Misasa spring water

International Nuclear Information System (INIS)

Nakamura, Mariko; Esaka, Takao; Kamata, Masahiro

2005-01-01

Hoping that use of natural radioactivity as teaching materials helps learners to understand the existence of radiation in nature, the authors developed several kinds of safe and inexpensive experiments for elementary and junior high school education using hot spring water taken from Misasa, situated in Tottori prefecture, Japan. Here, they report the details of experimental procedure to observe the radioactive equilibrium between Rn 222 released from the hot spring water and its daughters as well as the decay after isolation from Rn 222. The experiment needs no hazardous chemicals nor Bunsen burners, and can be carried out in normal classrooms without any special apparatus. (S. Ohno)

Annual absorbed dose rate at the surface of 38 hot and mineral springs in Iran

Energy Technology Data Exchange (ETDEWEB)

Bahreyni Toosi, M.; Orougi, M.H.; Sadeghzadeh, A.; Aghamir, A.; Jomehzadeh, A.; Zare, H. [Mashhad Univ. of Medical Sciences, Medical Physics Dep., Faculty of Medicine (Iran, Islamic Republic of)

2006-07-01

Full text of publication follows: Measurement of background radiation is very important from different points of view especially to human health. In some cases exposure rate near hot and mineral springs are higher than those of normal areas. The high background radiation of hot and mineral springs is primarily due to the presence of very high amounts of Ra 226 and its decay products. In this research, environmental gamma radiation of hot and mineral springs in Khorasan, Mazandaran and Sareeyn town in Ardabil province have been measured. Equipment used in this work included: a survey meter (R.D.S. -110), a tripod and an aluminium frame to hold the survey meter horizontally.R.D.S. -110 is a microprocessor controlled detector. This survey meter has been designed for monitoring X and rays and radiation. Measurements were carried out at one meter above water level in the vicinity of hot and mineral springs. Dose rates were recorded for one hour. The average of all recorded dose rates over one hour period was taken as the exposure rate for each station. The results indicate that in Khorasan province the highest and lowest annual absorbed dose rates were equal to 10.80 mSv/y at Shanigarmab and 0.52 mSv/y at Nasradin source respectively. In Mazandaran province maximum and minimum exposure rates equal to 54.4 and 0.53 mSv/y were obtained at the surface of Talleshmahalleh and Ghormerz sources. Exposure rates at the vicinity of Sarein sources were not very different and ranged from 1.39 to 1.59 mSv/y. The results indicate that in Khorasan province Shahingarmab hot spring has the highest annual absorbed dose rate (10.80 mSv/y) and Nasraddin in Sarbisheh has the lowest level of radiation (0.62 mSv/y). In Mazandaran province Taleshmahalleh hot mineral spring has the highest annual absorbed dose rate (54.41 mSv/y) and Ghormerz mineral spring has the lowest radiation level (0.53 mSv/y). Also in Sareeyn (in Ardabil province) Abechashm source has the highest annual absorbed dose

Thermal springs of Malaysia and their potentialdevelopment

Science.gov (United States)

Rahim Samsudin, Abdul; Hamzah, Umar; Rahman, Rakmi Ab.; Siwar, Chamhuri; Fauzi Mohd. Jani, Mohd; Othman, Redzuan

The study on the potential development of hot springs for the tourism industry in Malaysiawas conducted. Out of the 40 hot springs covered, the study identified 9 hot springs having a high potential for development, 14 having medium potential and the remaining 17 having low or least potential for development. This conclusion was arrived at after considering the technical and economic feasibility of the various hot springs. Technical feasibility criteria includes geological factors, water quality, temperature and flow rate. The economic feasibility criteria considers measures such as accessibility, current and market potentials in terms of visitors, surrounding attractions and existing inventory and facilities available. A geological input indicates that high potential hot springs are located close to or within the granite body and associated with major permeable fault zones. They normally occur at low elevation adjacent to topographic highs. High potential hot springs are also characterised by high water temperature, substantial flowrate and very good water quality which is important for water-body contact activities such as soaking. Economic criteria for high potential hot springs are associated with good accessibility, good market, good surrounding attractions like rural and village setting and well developed facilities and infrastructures.

Microbial diversity and autotrophic activity in Kamchatka hot springs.

Science.gov (United States)

Merkel, Alexander Yu; Pimenov, Nikolay V; Rusanov, Igor I; Slobodkin, Alexander I; Slobodkina, Galina B; Tarnovetckii, Ivan Yu; Frolov, Evgeny N; Dubin, Arseny V; Perevalova, Anna A; Bonch-Osmolovskaya, Elizaveta A

2017-03-01

Microbial communities of Kamchatka Peninsula terrestrial hot springs were studied using molecular, radioisotopic and cultural approaches. Analysis of 16S rRNA gene fragments performed by means of high-throughput sequencing revealed that aerobic autotrophic sulfur-oxidizing bacteria of the genus Sulfurihydrogenibium (phylum Aquificae) dominated in a majority of streamers. Another widely distributed and abundant group was that of anaerobic bacteria of the genus Caldimicrobium (phylum Thermodesulfobacteria). Archaea of the genus Vulcanisaeta were abundant in a high-temperature, slightly acidic hot spring, where they were accompanied by numerous Nanoarchaeota, while the domination of uncultured Thermoplasmataceae A10 was characteristic for moderately thermophilic acidic habitats. The highest rates of inorganic carbon assimilation determined by the in situ incubation of samples in the presence of 14 C-labeled bicarbonate were found in oxygen-dependent streamers; in two sediment samples taken from the hottest springs this process, though much weaker, was found to be not dependent on oxygen. The isolation of anaerobic lithoautotrophic prokaryotes from Kamchatka hot springs revealed a wide distribution of the ability for sulfur disproportionation, a new lithoautotrophic process capable to fuel autonomous anaerobic ecosystems.

The main chemical properties of hot and cold mineral waters in Bayankhongor, Mongolia

Directory of Open Access Journals (Sweden)

D Oyuntsetseg

2014-12-01

Full Text Available In the current study, hot and cold mineral springs and sub mineral waters in the Bayankhongor province were examined for their chemical characteristics and identified cold mineral waters classification according to mineral water classification of Mongolia. The hot spring waters belong to Na+-HCO3- and Na+-SO42- types. The cold mineral spring of Lkham belongs to Ca2+-HCO3- type. All sub mineral waters are generally located in the two areas (northern part or mountain forest area and the southern part or Gobi desert area. TDS concentrations of cold springs of the southern part in the study area were higher than northern part’s cold springs. The total dissolved silica content of cold spring was ranged from 4.5mg/L to 26 mg/L which did not correspond to requirements of mineral water standard of Mongolia. Thus, these cold springs are belonging to sub mineral water classification. The sub mineral waters were characterized into four types such as a Ca2+-SO42-, Na+-SO42-, Na+-HCO3 and Ca2+ - HCO3 by their chemical composition in the study area. The values for the quartz, chalcedony geothermometer and the Na/K geothermometer were quite different. The silica-enthalpy mixing model predicts a subsurface reservoir temperature between 124 and 197°C and most of the hot waters have been  probably mixed with cold water. The result shows that an averaged value of calculated temperature ranges from 77°C to 119°C which indicates that studied area has low temperature geothermal resources. DOI: http://doi.dx.org/10.5564/mjc.v15i0.324 Mongolian Journal of Chemistry 15 (41, 2014, p56-62

Diversity and Ecological Functions of Crenarchaeota in Terrestrial Hot Springs of Tengchong, China

Science.gov (United States)

Li, W.; Song, Z.; Chen, J.; Jiang, H.; Zhou, E.; Wang, F.; Xiao, X.; Zhang, C.

2010-12-01

The diversity and potential ecological functions of Crenarchaeota were investigated in eight terrestrial hot springs (pH: 2.8-7.7; temperature: 43.6-96 C) located in Tengchong, China, using 16S rRNA gene phylogenetic analysis. A total of 826 crenarchaeotal clones were analyzed and a total of 47 Operational taxonomic units (OTUs) were identified. Most (93%) of the identified OTUs were closely related (89-99%) to those retrieved from hot springs and other thermal environments. Our data showed that temperature may predominate over pH in affecting crenarchaeotal diversity in Tengchong hot springs. Crenarchaeotal diversity in moderate-temperature (59 to 77 C) hot springs was the highest, indicating that the moderate-temperature hot springs are more inclusive for Crenarchaeota. To understand what ecological functions these Crenarchaeota may play in Tengchong hot springs, we isolated the environmental RNA and constructed four cDNA clone libraries of the archaeal accA gene that encodes Acetyl CoA carboxylase. The accA gene represents one of the key enzymes responsible for the CO2 fixation in the 3-hydroxypropionate/4-hydroxybutyrate pathway. The results of phylogenetic analysis showed all the transcribed accA gene sequences can be classified into three large clusters, with the first one being affiliated with marine crenarchaeota, the second one with cultured crenarchaeota, and the third one with Chlorobi (Green sulfur bacteria), which have been proved to employ the 3-hydroxypropionate/4-hydroxybutyrate pathway. The long-branch distances of the phylogenetic tree suggest that these sequences represent novel accA-like gene. Our results also showed that sequences of the accA-like gene from the same hot spring belonged to one cluster, which suggests that a single crenarchaeotal group may fix CO2 via 3-hydroxypropionate/4-hydroxybutyrate pathway in the investigated hot springs.

Geochemistry of hydrothermal alteration at the Roosevelt Hot Springs thermal area, Utah

Energy Technology Data Exchange (ETDEWEB)

Parry, W T; Ballantyne, J M; Bryant, N L; Dedolph, R E

1980-01-01

Hot spring deposits in the Roosevelt thermal area consist of opaline sinter and sinter-cemented alluvium. Alluvium, plutonic rocks, and amphibolite-facies gneiss have been altered by acid-sulfate water to alunite and opal at the surface, and alunite, kaolinite, montmorillonite, and muscovite to a depth of 70 m. Marcasite, pyrite, chlorite, and calcite occur below the water table at about 30 m. The thermal water is dilute (ionic strength 0.1 to 0.2) sodium-chloride brine. The spring water now contains 10 times as much Ca, 100 times as much Mg, and up to 2.5 times as much SO/sub 4/ as the deep water. Although the present day spring temperature is 25/sup 0/C, the temperature was 85/sup 0/C in 1950. A model for development of the observed alteration is supported by observation and irreversible mass transfer calculations. Hydrothermal fluid convectively rises along major fractures. Water cools by conduction and steam separation, and the pH rises due to carbon dioxide escape. At the surface, hydrogen and sulfate ions are produced by oxidation of H/sub 2/S. The low pH water percolates downward and reacts with feldspar in the rocks to produce alunite, kaolinite, montmorillonite, and muscovite as hydrogen ion is consumed. 4 figures, 4 tables.

Hot spring deposits on a cliff face: A case study from Jifei, Yunnan Province, China

Science.gov (United States)

Jones, Brian; Peng, Xiaotong

2014-04-01

A cliff face in the Jifei karst area, southwest China, is covered by a spectacular succession of precipitates that formed from the hot spring water that once flowed down its surface. This layered succession is formed of aragonite layers that are formed largely of “fountain dendrites”, calcite layers that are formed mostly of “cone dendrites”, and microlaminated layers that contain numerous microbes and extracellular polymeric substances (EPS). Many of the aragonite crystals are hollow due to preferential dissolution of their cores. The calcite cone dendrites are commonly covered with biofilms, reticulate Si-Mg coatings, and other precipitates. The microbial layers include dodecahedral calcite crystals and accessory minerals that include opal-A, amorphous Si-Mg coatings, trona, barite, potassium sulfate crystals, mirabillite, and gaylussite. Interpretation of the δ18O(calcite) and δ18O(aragonite) indicates precipitation from water with a temperature of 54 to 66 °C. The active hot spring at the top of the cliff presently ejects water at a temperature of 65 °C. Layers, 1 mm to 6 cm thick, record temporal changes in the fluids from which the precipitates formed. This succession is not, however, formed of recurring cycles that can be linked to diurnal or seasonal changes in the local climate. Indeed, it appears that the climatic contrast between the wet season and the dry season had little impact on precipitation from the spring waters that flowed down the cliff face. Integration of currently available evidence suggests that the primary driving force was aperiodic changes in the CO2 content of the spring waters because that seems to be the prime control on the saturation levels that underpinned precipitation of the calcite and aragonite as well as the dissolution of the aragonite. Such variations in the CO2 content of the spring water were probably due to changes that took place in the subterranean plumbing system of the spring.

Microbial community analysis of a coastal hot spring in Kagoshima, Japan, using molecular- and culture-based approaches.

Science.gov (United States)

Nishiyama, Minako; Yamamoto, Shuichi; Kurosawa, Norio

2013-08-01

Ibusuki hot spring is located on the coastline of Kagoshima Bay, Japan. The hot spring water is characterized by high salinity, high temperature, and neutral pH. The hot spring is covered by the sea during high tide, which leads to severe fluctuations in several environmental variables. A combination of molecular- and culture-based techniques was used to determine the bacterial and archaeal diversity of the hot spring. A total of 48 thermophilic bacterial strains were isolated from two sites (Site 1: 55.6°C; Site 2: 83.1°C) and they were categorized into six groups based on their 16S rRNA gene sequence similarity. Two groups (including 32 isolates) demonstrated low sequence similarity with published species, suggesting that they might represent novel taxa. The 148 clones from the Site 1 bacterial library included 76 operational taxonomy units (OTUs; 97% threshold), while 132 clones from the Site 2 bacterial library included 31 OTUs. Proteobacteria, Bacteroidetes, and Firmicutes were frequently detected in both clone libraries. The clones were related to thermophilic, mesophilic and psychrophilic bacteria. Approximately half of the sequences in bacterial clone libraries shared <92% sequence similarity with their closest sequences in a public database, suggesting that the Ibusuki hot spring may harbor a unique and novel bacterial community. By contrast, 77 clones from the Site 2 archaeal library contained only three OTUs, most of which were affiliated with Thaumarchaeota.

Comparative metagenomics of eight geographically remote terrestrial hot springs

DEFF Research Database (Denmark)

Menzel, Peter; Islin, Sóley Ruth; Rike, Anne Gunn

2015-01-01

Hot springs are natural habitats for thermophilic Archaea and Bacteria. In this paper, we present the metagenomic analysis of eight globally distributed terrestrial hot springs from China, Iceland, Italy, Russia, and the USA with a temperature range between 61 and 92 (∘)C and pH between 1.8 and 7....... A comparison of the biodiversity and community composition generally showed a decrease in biodiversity with increasing temperature and decreasing pH. Another important factor shaping microbial diversity of the studied sites was the abundance of organic substrates. Several species of the Crenarchaeal order...

Diagenetic Changes in Common Hot Spring Microfacies

Science.gov (United States)

Hinman, N. W.; Kendall, T. A.; MacKenzie, L. A.; Cady, S. D.

2016-05-01

The friable nature of silica hot spring deposits makes them susceptible to mechanical weathering. Rapid diagenesis must take place for these rocks to persist in the geologic record. The properties of two microfacies at two deposits were compared.

How important is hydrotherapy? Effects of dynamic action of hot spring water as a rehabilitative treatment for burn patients in Switzerland.

Science.gov (United States)

Moufarrij, S; Deghayli, L; Raffoul, W; Hirt-Burri, N; Michetti, M; de Buys Roessingh, A; Norberg, M; Applegate, L A

2014-12-31

Burn rehabilitation using hydrotherapy can have multiple benefits for the burn patient. The therapy uses specific mineral enriched hot spring water and water jets with varied hydro-pressure to combat hypertrophy, inflammatory reaction signs, abnormal pigmentation, and, more specifically, redness and scarring. Standard operating procedures for burn rehabilitation have been developed and integrated into the Standard of Care at the CHUV hospital using localized hydro-mechanical stimulation of burn sites (20 minutes of alternating anatomical sites) followed by constant pressure large-bore and filiform showers targeting specific scarred areas. These therapeutic regimens are repeated daily for 2 to 3 weeks. Patients showed lasting effects from this regimen (up to 3-6 months), the results becoming permanent with more uniform skin structure, color and visco-elasticity in addition to a decrease in pruritus. The specifications of clinical protocols are described herein along with the virtues of hot spring hydro-pressure therapy for burn rehabilitation. The use of hydrotherapy, which has been a controversial topic among burn units across the world, is also discussed. In North America, hydrotherapy is defined only within the scope of in-patient wound cleansing and is thought to lead to microbial auto-contamination and bacterial resistance. In Switzerland and France the emphasis of hydrotherapy is on rehabilitation after the wound has closed.

Geologic reconnaissance of the Hot Springs Mountains, Churchill County, Nevada

Science.gov (United States)

Voegtly, Nickolas E.

1981-01-01

A geologic reconnaissance of the Hot Springs Mountains and adjacent areas, which include parts of the Brady-Hazen and the Stillwater-Soda Lake Known Geothermal Resource Areas, during June-December 1975, resulted in a reinterpretation of the nature and location of some Basin and Range faults. In addition, the late Cenozoic stratigraphy has been modified, chiefly on the basis of radiometric dates of volcanic rocks by U.S. Geological Survey personnel and others. The Hot Springs Mountains are in the western part of the Basin and Range province, which is characterized by east-west crustal extension and associated normal faulting. In the surrounding Trinity, West Humboldt, Stillwater, and Desert Mountains, Cenozoic rocks overlie ' basement ' rocks of the Paleozoic and Mesozoic age. A similar relation is inferred in the Hot Springs Mountains. Folding and faulting have taken place from the late Tertiary to the present. (USGS)

Abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China.

Science.gov (United States)

Song, Zhao-Qi; Wang, Li; Wang, Feng-Ping; Jiang, Hong-Chen; Chen, Jin-Quan; Zhou, En-Min; Liang, Feng; Xiao, Xiang; Li, Wen-Jun

2013-09-01

It has been suggested that archaea carrying the accA gene, encoding the alpha subunit of the acetyl CoA carboxylase, autotrophically fix CO2 using the 3-hydroxypropionate/4-hydroxybutyrate pathway in low-temperature environments (e.g., soils, oceans). However, little new information has come to light regarding the occurrence of archaeal accA genes in high-temperature ecosystems. In this study, we investigated the abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China, using DNA- and RNA-based phylogenetic analyses and quantitative polymerase chain reaction. The results showed that archaeal accA genes were present and expressed in the investigated Yunnan hot springs with a wide range of temperatures (66-96 °C) and pH (4.3-9.0). The majority of the amplified archaeal accA gene sequences were affiliated with the ThAOA/HWCG III [thermophilic ammonia-oxidizing archaea (AOA)/hot water crenarchaeotic group III]. The archaeal accA gene abundance was very close to that of AOA amoA gene, encoding the alpha subunit of ammonia monooxygenase. These data suggest that AOA in terrestrial hot springs might acquire energy from ammonia oxidation coupled with CO2 fixation using the 3-hydroxypropionate/4-hydroxybutyrate pathway.

FY 2000 report on the survey for introduction of the hot spring effect prediction method in the geothermal development promotion survey. Improvement of the hot spring effect prediction method in the geothermal development promotion survey; 2000 nendo chinetsu kaihatsu sokushin chosa. Onsen eikyo yosoku shuho donyu chosa - Chinetsu kaihatsu sokushin chosa ni okeru onsen eikyo yosoku shuho no kairyo hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

Supposing the case where the geothermal development promotion survey was carried out in promising geothermal areas in Japan, investigational study was conducted on possibilities of introducing the hot spring effect prediction method, improvement of the method, etc. In the survey, adjustment/classification of formation mechanisms of hot spring were made. For each of the formation mechanisms, the mechanisms in case of the geothermal development having effects were studied/summarized. As to how effects are brought about, presumed were the lowering of water level and decrease in discharge amount in accordance with the decreasing pressure and the dilution by increase in mixture of the ground water around the area. Also cited were the vaporization of hot spring aquifers by the increasing rate of vapor inflow, etc. For the introduction of the hot spring effect prediction method to the geothermal development promotion survey, the problem is short supply of various data, and the examination for it was made. Based on the results of the survey, items to be studied in case of introducing the hot spring effect prediction method were selected. Further, the hot spring effect prediction flow in case of introducing surface survey and well survey was made out. (NEDO)

High Prevalence, Genetic Diversity and Intracellular Growth Ability of Legionella in Hot Spring Environments

Science.gov (United States)

Zhou, Haijian; Wang, Huanxin; Xu, Ying; Zhao, Mingqiang; Guan, Hong; Li, Machao; Shao, Zhujun

2013-01-01

Background Legionella is the causative agent of Legionnaires' disease, and hot springs are a major source of outbreaks of this disease. It is important from a public health perspective to survey hot spring environments for the presence of Legionella. Methods Prospective surveillance of the extent of Legionella pollution was conducted at three hot spring recreational areas in Beijing, China in 2011. Pulsed-field gel electrophoresis (PFGE) and sequence-based typing (SBT) were used to describe the genetic polymorphism of isolates. The intracellular growth ability of the isolates was determined by interacting with J774 cells and plating the dilutions onto BCYE agar plates. Results Overall, 51.9% of spring water samples showed Legionella-positive, and their concentrations ranged from 1 CFU/liter to 2,218 CFU/liter. The positive rates of Legionella were significantly associated with a free chlorine concentration of ≥0.2 mg/L, urea concentration of ≥0.05 mg/L, total microbial counts of ≥400 CFU/ml and total coliform of ≥3 MPN/L (pLegionella concentrations were significantly associated with sample temperature, pH, total microbial counts and total coliform (pLegionella pneumophila was the most frequently isolated species (98.9%), and the isolated serogroups included serogroups 3 (25.3%), 6 (23.4%), 5 (19.2%), 1 (18.5%), 2 (10.2%), 8 (0.4%), 10 (0.8%), 9 (1.9%) and 12 (0.4%). Two hundred and twenty-eight isolates were analyzed by PFGE and 62 different patterns were obtained. Fifty-seven L. pneumophila isolates were selected for SBT analysis and divided into 35 different sequence types with 5 main clonal groups. All the 57 isolates had high intracellular growth ability. Conclusions Our results demonstrated high prevalence and genetic polymorphism of Legionella in springs in Beijing, China, and the SBT and intracellular growth assay results suggested that the Legionella isolates of hot spring environments were pathogenic. Improved control and prevention strategies are

High prevalence, genetic diversity and intracellular growth ability of Legionella in hot spring environments.

Directory of Open Access Journals (Sweden)

Tian Qin

Full Text Available BACKGROUND: Legionella is the causative agent of Legionnaires' disease, and hot springs are a major source of outbreaks of this disease. It is important from a public health perspective to survey hot spring environments for the presence of Legionella. METHODS: Prospective surveillance of the extent of Legionella pollution was conducted at three hot spring recreational areas in Beijing, China in 2011. Pulsed-field gel electrophoresis (PFGE and sequence-based typing (SBT were used to describe the genetic polymorphism of isolates. The intracellular growth ability of the isolates was determined by interacting with J774 cells and plating the dilutions onto BCYE agar plates. RESULTS: Overall, 51.9% of spring water samples showed Legionella-positive, and their concentrations ranged from 1 CFU/liter to 2,218 CFU/liter. The positive rates of Legionella were significantly associated with a free chlorine concentration of ≥0.2 mg/L, urea concentration of ≥0.05 mg/L, total microbial counts of ≥400 CFU/ml and total coliform of ≥3 MPN/L (p<0.01. The Legionella concentrations were significantly associated with sample temperature, pH, total microbial counts and total coliform (p<0.01. Legionella pneumophila was the most frequently isolated species (98.9%, and the isolated serogroups included serogroups 3 (25.3%, 6 (23.4%, 5 (19.2%, 1 (18.5%, 2 (10.2%, 8 (0.4%, 10 (0.8%, 9 (1.9% and 12 (0.4%. Two hundred and twenty-eight isolates were analyzed by PFGE and 62 different patterns were obtained. Fifty-seven L. pneumophila isolates were selected for SBT analysis and divided into 35 different sequence types with 5 main clonal groups. All the 57 isolates had high intracellular growth ability. CONCLUSIONS: Our results demonstrated high prevalence and genetic polymorphism of Legionella in springs in Beijing, China, and the SBT and intracellular growth assay results suggested that the Legionella isolates of hot spring environments were pathogenic. Improved control

Hot spring therapy of the patients exposed to atomic bomb radiation, 15

Energy Technology Data Exchange (ETDEWEB)

Ouchi, Tamon [Genbaku Hibakusha Beppu Onsen Ryoyo Kenkyusho, Oita (Japan); Tsuji, Hideo

1983-03-01

The patients exposed to the atomic bomb radiation in Hiroshima area came to Beppu Spa to have hot spring therapy. During the fiscal year of 1982 (April, 1982, to March, 1983), 3972 persons came to the hot spring sanatorium, and 586 patients (14.8 %) received physical examination. Among them, 473 patients (80.7 %) were exposed to the atomic bomb radiation on August 6, 1945, or entered in the city of Hiroshima by August 20, 1945, according to the official notebook issued by the government. Physical examination was performed twice a week during their stay, and more than 53.5 % of the patients were older than 70, and the oldest was 93 years old. Blood pressure was measured when the patients came in and went out, and hypertensive patients were asked to observe the rule of treatment strictly. The complaints of the patients which brought them to the hot spring were mostly pain in bodies and lower extremities, and hypertension, common cold syndrome, diabetes and constipation. Patients took hot spring bath 2

Geofluids Assessment of the Ayub and Shafa Hot Springs in Kopet-Dagh Zone (NE Iran: An Isotopic Geochemistry Approach

Directory of Open Access Journals (Sweden)

Hossein Mohammadzadeh

2017-01-01

Full Text Available Geothermal energy has a wide range of uses in our life. It is very important to characterize the temperature and the depth of geothermal reservoirs. The aim of this paper is the determination of type, origin source of water temperature, and depth of water circulation in the Ayub-Peighambar and Shafa (AP and SH hot springs, located in NE Iran, using hydrogeochemistry and environmental isotopes (2H and 18O. AP hot spring has elevated temperature (36–40°C and as such is very important for balneotherapy and geotourism industry purposes. The average values of δ18O and δ2H for this hot spring (−10‰ and −73‰, resp. are analogous to that of geothermal and meteoric waters. This indicates that the heat source cannot be related to volcanic activities (with average δ18O value of about 5‰ and it is most probably associated with geothermal gradient with deep circulation of groundwater through faults. Based on Na-K geothermometers coupled with isotopic (18O and 2H geochemistry the temperature of the AP geothermal reservoir was estimated to be in the range of 100–150°C with 3–5 and 4.2 kilometres’ depth, respectively. Chemically, the AP samples are CaSO4 facies with a chemically homogeneous source and steam heated waters type.

Nitrogen cycling in Hot Spring Sediments and Biofilms (Invited)

Science.gov (United States)

Meyer-Dombard, D. R.; Burton, M. S.; Havig, J. R.; Shock, E.

2010-12-01

Over the past several decades, gene-targeted analyses have revealed that microbial communities in hydrothermal environments can be surprisingly diverse. However, we know shockingly little about basic ecological functions such as carbon and nitrogen cycling or community shifts over time, or environmental parameters such as growth criteria. Previous work has shown that carbon cycling in one hot spring in Yellowstone National Park [“Bison Pool”] and its associated runoff channel functions as a complex system. Analysis of carbon and nitrogen isotopes in biofilms across a temperature and chemical gradient at this location revealed that multiple autotrophic carbon fixation pathways are functioning in this system, and nitrogen fixation varies across the chemosynthetic/photosynthetic ecotone [1]. Further, sequencing of metagenomes from multiple locations at “Bison Pool” has indicated the presence of genes involved in carbon fixation [both phototrophic and autotrophic], and heterotrophy, as well as nitrogen fixation [2]. Studies from other Yellowstone locations have also found genetic evidence for carbon and nitrogen fixation [3-5]. The role of individual microbes in nitrogen cycling as environmental conditions vary over space and time is the focus of this study. Here, we explore the diversity of nifH [nitrogen fixation], nirK [nitrite reduction] and amoA [ammonia oxidation] genes across a variety of Yellowstone environments. Environmental nucleic acids were extracted, and the presence/absence of Bacteria and Archaea determined by PCR. In addition, PCR-directed screens reveal the presence or absence of the aforementioned functional genes, indicating genetic capacity for nitrogen cycling. We have examined the transition of genetic diversity and genetic capacity within sediments and biofilms at the chemosynthetic/photosynthetic ecotone in several hot springs spanning ranges of pH and geochemical conditions. By sampling across this ecotone, changes in the genetic

Change in color of the hot spring deposits at the Chinoike-Jigoku hot pool, Beppu geothermal field

Energy Technology Data Exchange (ETDEWEB)

Kazuthoshi, Oue; Ohsawa, Shinji; Yusa, Yuki [Kyoto University, Beppu (Japan). Beppu Geothermal Research Laboratory, Graduate School of Science

2002-06-01

The Chinoike-Jigoku hot pool in Beppu geothermal field, Central Kyushu, Japan, displays a blood-red color due to the hematite (Fe{sub 2}O{sub 3}) deposited at the bottom of the pool. The colors of the deposits collected on 1 October 1990, on 27 March 1995, and on 6 March 1996 were measured with a colorimeter. The results show that the red deposits became yellower in 1995 and 1996 than they were in 1990. X-ray diffraction (XRD) patterns and chemical compositions of the deposits indicate that the discoloration of the Chinoike-Jigoku pool water is caused by an increase in the content of jarosite [KFe{sub 3}(SO{sub 4}){sub 2}(OH){sub 6}]. The temperature of the subsurface thermal water beneath the Chinoike-Jigoku hot pool, as estimated by the anhydrite chemical geothermometer, has declined from 200 to 150{sup o}C over the past 25 years. The Na and Cl concentrations of the hot spring water discharging from Chinoike-Jigoku have decreased, while the SO{sub 4} concentration has increased. The temporal variations in subsurface temperature and dissolved ion concentrations suggest that the mixing ratio between the high-temperature, neutral Na-Cl type water and the relatively low-temperature, acid H-SO{sub 4} type water that form the thermal water of Chinoike-Jigoku has changed over the last 25 years. Hydrothermal studies of jarosite stability have confirmed that the increase in jarosite content in the deposits was caused by a temperature drop of the mixed thermal water beneath Chinoike-Jigoku pool, due to an increase in the contribution of the cooler H-SO{sub 4} water type to the thermal mixture. (author)

Mining Hot Springs for Biodiversity and Novel Enzymes

DEFF Research Database (Denmark)

Islin, Sóley Ruth

organisms have proven to be a great source of novel enzymes that are valuable in a variety of industrial processes. We set out to search for novel thermophilic hydrolytic enzymes by taking samples from thermal environments around the world. We employed several different methods in achieving this, both......The existence of microbial life at extreme environments, such as hot springs, has been known for a few decades. The remarkable ability of microorganisms to withstand the extreme conditions of their habitats, has astounded scientist and pushed the limits of what was considered possible. Thermophilic...... culture-dependent as well as culture-independent methods. Each hot spring sample was enriched on various polymeric substrates at high temperatures in the search of thermophilic microorganism with the ability to degrade the substrate. Enzymatic activity of the cultures was confirmed, the most promising...

Lipid Biomarkers and Stable Isotope Signatures of Microbial Mats in Hot Springs of Kamchatka, Russia

Science.gov (United States)

Romanek, C. S.; Mills, G. L.; Jones, M. E.; Paddock, L.; Li, Y.; Zhang, C. L.; Wiegel, J.

2004-12-01

Various hot springs of the Uzon Caldera, Kamchatka, were analyzed for their chemical and stable isotope composition to better understand the relationship(s) between thermophilic microorganisms and the environments in which they live. The springs had water temperatures ranging from 40-90\\deg C and pH ranging from 5.6-5.9. Gases that emanated from the springs were composed predominantly of CO2 (20 to 90%), with lesser amounts of CH4, (Archaea. Results of PLFA showed 16:0 as the most abundant fatty acid (33-44%), which is universal in all living organisms. Other significant biomarkers included 18:1ω (19 to 24%), 18:2ω (5 to 13%), 16:1ω (3 to 12%), and 18:0 (2 to 7%). These biomarkers are characteristic of cyanobacteria, green-sulfur bacteria, and green non-sulfur bacteria, respectively, which are common autotrophic organisms in terrestrial hot springs. On the other hand, biomarkers of heterotrophic bacteria, such as iso- and anteiso-15:0 were low (2-8%), indicating that the bacterial carbon cycle was dominated by autotrophic organisms. Analogous archaeal constituents were present in significant abundance in the ether lipids fraction.

Phylogenetic Analysis and Antimicrobial Profiles of Cultured Emerging Opportunistic Pathogens (Phyla Actinobacteria and Proteobacteria) Identified in Hot Springs.

Science.gov (United States)

Jardine, Jocelyn Leonie; Abia, Akebe Luther King; Mavumengwana, Vuyo; Ubomba-Jaswa, Eunice

2017-09-15

Hot spring water may harbour emerging waterborne opportunistic pathogens that can cause infections in humans. We have investigated the diversity and antimicrobial resistance of culturable emerging and opportunistic bacterial pathogens, in water and sediment of hot springs located in Limpopo, South Africa. Aerobic bacteria were cultured and identified using 16S ribosomal DNA (rDNA) gene sequencing. The presence of Legionella spp. was investigated using real-time polymerase chain reaction. Isolates were tested for resistance to ten antibiotics representing six different classes: β-lactam (carbenicillin), aminoglycosides (gentamycin, kanamycin, streptomycin), tetracycline, amphenicols (chloramphenicol, ceftriaxone), sulphonamides (co-trimoxazole) and quinolones (nalidixic acid, norfloxacin). Gram-positive Kocuria sp. and Arthrobacter sp. and gram-negative Cupriavidus sp., Ralstonia sp., Cronobacter sp., Tepidimonas sp., Hafnia sp. and Sphingomonas sp. were isolated, all recognised as emerging food-borne pathogens. Legionella spp. was not detected throughout the study. Isolates of Kocuria , Arthrobacter and Hafnia and an unknown species of the class Gammaproteobacteria were resistant to two antibiotics in different combinations of carbenicillin, ceftriaxone, nalidixic acid and chloramphenicol. Cronobacter sp. was sensitive to all ten antibiotics. This study suggests that hot springs are potential reservoirs for emerging opportunistic pathogens, including multiple antibiotic resistant strains, and highlights the presence of unknown populations of emerging and potential waterborne opportunistic pathogens in the environment.

Clumped isotopologue constraints on the origin of methane at seafloor hot springs

Science.gov (United States)

Wang, David T.; Reeves, Eoghan P.; McDermott, Jill M.; Seewald, Jeffrey S.; Ono, Shuhei

2018-02-01

Hot-spring fluids emanating from deep-sea vents hosted in unsedimented ultramafic and mafic rock commonly contain high concentrations of methane. Multiple hypotheses have been proposed for the origin(s) of this methane, ranging from synthesis via reduction of aqueous inorganic carbon (∑CO2) during active fluid circulation to leaching of methane-rich fluid inclusions from plutonic rocks of the oceanic crust. To further resolve the process(es) responsible for methane generation in these systems, we determined the relative abundances of several methane isotopologues (including 13CH3D, a "clumped" isotopologue containing two rare isotope substitutions) in hot-spring source fluids sampled from four geochemically-distinct hydrothermal vent fields (Rainbow, Von Damm, Lost City, and Lucky Strike). Apparent equilibrium temperatures retrieved from methane clumped isotopologue analyses average 310-42+53 °C, with no apparent relation to the wide range of fluid temperatures (96-370 °C) and chemical compositions (pH, [H2], [∑CO2], [CH4]) represented. Combined with very similar bulk stable isotope ratios (13C/12C and D/H) of methane across the suite of hydrothermal fluids, all available geochemical and isotopic data suggest a common mechanism of methane generation at depth that is disconnected from active fluid circulation. Attainment of equilibrium amongst methane isotopologues at temperatures of ca. 270-360 °C is compatible with the thermodynamically-favorable reduction of CO2 to CH4 at temperatures at or below ca. 400 °C under redox conditions characterizing intrusive rocks derived from sub-ridge melts. Collectively, the observations support a model where methane-rich aqueous fluids, known to be trapped in rocks of the oceanic lithosphere, are liberated from host rocks during hydrothermal circulation and perhaps represent the major source of methane venting with thermal waters at unsedimented hydrothermal fields. The results also provide further evidence that water

Investigation of geothermal development and promotion for fiscal 1997. Fluid geochemical investigation (hot-spring gas) report (No. B-5 Musadake area); 1997 nendo chinetsu kaihatus sokushin chosa. Ryutai chikagaku chosa (onsen gas) hokokusho (No.B-5 Musadake chiiki)

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-03-01

This investigation elucidated the possible existence of geothermal reservoir layer in the subject area by studying and analyzing the hot-spring gasses of Musadake. The Musadake area is the one extending over Shibetu-cho and Nakashibetu-cho, Shibetu district, Hokkaido. The sampling of the hot-spring gasses was carried out at three natural gusher sites and one hot spring well site. The gasses in the Kawakita hot spring is most affected by volcanic gasses. The origin of the volcanic gasses is a magmatic gas of andesite nature the {sup 3}He/{sup 4}He ratio of which is 8X10{sup -6} or about. As a result of the analysis, the hot-spring water is Na-Cl type, high salt concentrated, and 200 degrees C in temperature; from the result of a gas geochemical thermometer, it is estimated to be not less than 250 degrees C. In the tectonic viewpoint, the depth hot water is derived from the meteorite water that flows in through a bent zone incident to the Musadake-Shitabanupuri mountain fault and from the fossil sea water that exists in the underground depth; the depth hot water is formed by conduction heat from a magma reservoir that formed Musadake and by volcanic ejecta. This depth hot water rises along Kawakita south, Urappu River fault, etc., mixing with the meteorite water and forming the shallow reservoir layer. (NEDO)

Archaeal and bacterial community analysis of several Yellowstone National Park hot springs

Science.gov (United States)

Colman, D. R.; Takacs-Vesbach, C. D.

2012-12-01

The hot springs of Yellowstone National Park (YNP) are home to a diverse assemblage of microorganisms. Culture-independent studies have significantly expanded our understanding of the diversity of both Bacteria and Archaea present in YNP springs as well as the geochemical and ecological controls on communities. While the ecological analysis of Bacteria among the physicochemically heterogenous springs of YNP has been previously conducted, less is known about the extent of diversity of Archaeal communities and the chemical and ecological controls on their populations. Here we report a culture-independent analysis of 31 hot spring archaeal and bacterial communities of YNP springs using next generation sequencing. We found the phylogenetic diversity of Archaea to be generally comparable to that of co-occurring bacterial communities although overall, in the springs we investigated, diversity was higher for Bacteria than Archaea. Chemical and physical controls were similar for both domains with pH correlating most strongly with community composition. Community differences reflected the partitioning of taxonomic groups in low or high pH springs for both domains. Results will be discussed in a geochemical and ecological context.

Portrait of a Geothermal Spring, Hunter's Hot Springs, Oregon.

Science.gov (United States)

Castenholz, Richard W

2015-01-27

Although alkaline Hunter's Hot Springs in southeastern Oregon has been studied extensively for over 40 years, most of these studies and the subsequent publications were before the advent of molecular methods. However, there are many field observations and laboratory experiments that reveal the major aspects of the phototrophic species composition within various physical and chemical gradients of these springs. Relatively constant temperature boundaries demark the upper boundary of the unicellular cyanobacterium, Synechococcus at 73-74 °C (the world-wide upper limit for photosynthesis), and 68-70 °C the upper limit for Chloroflexus. The upper limit for the cover of the filamentous cyanobacterium, Geitlerinema (Oscillatoria) is at 54-55 °C, and the in situ lower limit at 47-48 °C for all three of these phototrophs due to the upper temperature limit for the grazing ostracod, Thermopsis. The in situ upper limit for the cyanobacteria Pleurocapsa and Calothrix is at ~47-48 °C, which are more grazer-resistant and grazer dependent. All of these demarcations are easily visible in the field. In addition, there is a biosulfide production in some sections of the springs that have a large impact on the microbiology. Most of the temperature and chemical limits have been explained by field and laboratory experiments.

Thermal algae in certain radioactive springs in Japan, (3)

International Nuclear Information System (INIS)

Mifune, Masaaki; Hirose, Hiroyuki.

1982-01-01

Shikano Hot Springs are located at five km to the south of Hamamura Station on the Sanin Line in Tottori Prefecture. The water temperature and the pH of the springs are 40.2 - 61.2 0 C, and 7.5 - 7.8, respectively. They belong to simple thermals. Hamamura Hot Springs are located in the neighbourhood of Hamamura Station. The highest radon content of the hot springs is 175.1 x 10 -10 Ci/l, and the great part of the springs belong to radioactive ones. From the viewpoint of the major ionic constituents, they are also classified under weak salt springs, sulfated salt springs, and simple thermals. Regarding the habitates of the algal flora, the water temperature and the pH of the springs are 28.0 - 68.0 0 C, and 6.8 - 7.4, respectively. The thermal algae found by Ikoma and Doi at Hamamura Hot Springs were two species of Cyanophyceae. By the authors, nine species and one variety of Cyanophyceae including Ikoma and Doi's two species were newly found at Shikano and Hamamura Hot Springs. Chlorophyceous alga was not found. The dominant thermal algae of these hot springs were Mastigocladus laminosus, and the other algae which mainly consist of Oscillatoriaceous algae. From these points, it seems that the thermal algae of Shikano and Hamamura Hot Springs belong to the normal type of thermal algae, and they are different from the thermal algae of Ikeda Mineral Springs and Masutomi Hot Springs which belong to strongly radioactive springs. (author)

Metagenomic analysis of bacterial diversity of Siloam hot water ...

African Journals Online (AJOL)

The bacterial diversity of Siloam hot water spring was determined using 454 pyrosequencing of two 16S rRNA variable regions V1-3 and V4-7. Analysis of the community DNA revealed that the phyla Proteobacteria, Cyanobacteria, Bacteriodetes, Planctomycetes, Firmicutes, Chloroflexi and Verrucomicrobia were the most ...

Chemical characteristics of the major thermal springs of Montana

Energy Technology Data Exchange (ETDEWEB)

Mariner, R.H.; Presser, T.S.; Evans, W.C.

1976-07-01

Twenty-one thermal springs in western Montana were sampled for chemical, isotope, and gas compositions. Most of the springs issue dilute to slightly saline sodium-bicarbonate waters of neutral to slightly alkaline pH. A few of the springs issue sodium-mixed anion waters of near neutral pH. Fluoride concentrations are high in most of the thermal waters, up to 18 miligrams per litre, while F/Cl ratios range from 3/1 in the dilute waters to 1/10 in the slightly saline waters. Most of the springs are theoretically in thermodynamic equilibrium with respect to calcite and fluorite. Nitrogen is the major gas escaping from most of the hot springs; however, Hunters Hot Springs issue principally methane. The deuterium content of the hot spring waters is typical of meteoric water in western Montana. Geothermal calculations based on silica concentrations and Na-K-Ca ratios indicate that most of the springs are associated with low temperature aquifers (less than 100/sup 0/C). Chalcedony may be controlling the silica concentrations in these low temperature aquifers even in ''granitic'' terranes.

Residential solar hot water

Energy Technology Data Exchange (ETDEWEB)

1982-06-01

This report examines the feasibility of using solar energy to preheat domestic water coming from the city supply at a temperature of approximately 4{degree}C. Four solar collectors totalling 7 m{sup 2} were installed on a support structure facing south at an angle of 60{degree} from the horizontal. The system worked most efficiently in the spring and early summer when the combination of long hours of sunshine, clean air and clear skies allowed for maximum availability of solar radiation. Performance dropped in late summer and fall mainly due to cloudier weather conditions. The average temperature in the storage tank over the 10 months of operation was 42{degree}C, ranging from a high of 83{degree}C in July to a low of 6{degree}C in November. The system provided a total of 7.1 GJ, which is approximately one-third the annual requirement for domestic hot water heating. At the present time domestic use of solar energy to heat water does not appear to be economically viable. High capital costs are the main problem. As a solar system with present day technology can only be expected to meet half to two-thirds of the hot water energy demand the savings are not sufficient for the system to pay for itself within a few years. 5 figs.

Serratia sp. ZF03: an efficient radium biosorbent isolated from hot-spring waters in high background radiation areas of Ramsar

International Nuclear Information System (INIS)

Zakeri, F.; Sadeghizadeh, M.; Akbari Noghabi, K.; Farshidpour, M.R.; Kardan, M.R.; Atarilar, A.

2010-01-01

Natural radionuclides, in particular those emitting alpha particles, make the largest contribution to the world population exposure. The most important example is 226 Ra, with a high potency for causing biological damages. Accordingly, it can be a potential concern in many areas, where these elements have been existed naturally, mined or processed. In addition to its own radiological properties, radium isotopes present additional environmental and health concerns due to the fact that they decay into radon ( 222 Rn); a Class-A carcinogen and the second leading cause of lung cancer estimated to cause 21,000 deaths in the US annually. Physico-chemical methods have been widely used to remove radionuclides and heavy metal ions from wastewaters. These conventional methods may be ineffective or expensive with a few major disadvantages such as high energy requirements, incomplete removal and generation of toxic sludge which needs proper disposal. Biological treatment is an innovative technology available for heavy metal and radionuclide polluted wastewaters. The aim of this study was to isolate and characterize 226 Ra biosorbing indigenous bacterial strains from soils and hot-springs of Ramsar containing high concentration of 226 Ra by using biochemical and molecular approaches. Hence, the studied biomass proved very effective and could be used as a low cost and ecofriendly biosorbent for treatment of hot-spring waters containing high levels of 226 Ra in Ramsar

Utilization of Indonesia's Hot Spring Sources for Electricity using Kalina Cycle and Organic Rankine Cycle

Science.gov (United States)

Prabumukti, Grano; Purwanto; Widodo, Wahyu

2018-02-01

Indonesia posses 40% of the world's geothermal energy sources. The existence of hydrothermal sources is usually characterized by their surface manifestations such as hot springs, geysers and fumarole. Hot spring has a potential to be used as a heat source to generate electricity especially in a rural and isolated area. Hot springs can be converted into electricity by binary thermodynamic cycles such as Kalina cycle and ORC. The aim of this study is to obtain the best performances of cycle configuration and the potential power capacity. Simulation is conducted using UNISIM software with working fluid and its operating condition as the decision variables. The simulation result shows that R1234yf and propene with simple ORC as desired working fluid and cycle configuration. It reaches a maximum thermal efficiency up to 9.6% with a specific turbine inlet pressure. Higher temperature heat source will result a higher thermal efficiency‥ Cycle thermal efficiency varies from 4.7% to 9.6% depends on source of hot spring temperature. Power capacity that can be generated using Indonesia's hot spring is ranged from 2 kWe to 61.2 kWe. The highest capacity located in Kawah Sirung and the least located in Kaendi.

Investigation of bacterial diversity of hot springs of Odisha, India

Directory of Open Access Journals (Sweden)

Rajesh Kumar Sahoo

2015-12-01

Full Text Available 16S rRNA deep sequencing analysis, targeting V3 region was performed using Illumina bar coded sequencing. Sediment samples from two hot springs (Atri and Taptapani were collected. Atri and Taptapani metagenomes were classified into 50 and 51 bacterial phyla. Proteobacteria (45.17% dominated the Taptapani sample metagenome followed by Bacteriodetes (23.43% and Cyanobacteria (10.48% while in the Atri sample, Chloroflexi (52.39%, Nitrospirae (10.93% and Proteobacteria (9.98% dominated. A large number of sequences remained taxonomically unresolved in both hot springs, indicating the presence of potentially novel microbes in these two unique habitats thus unraveling the importance of the current study. Metagenome sequence information is now available at NCBI, SRA database accession no. SRP057428.

Hot spring therapy of the patients exposed to atomic bomb radiation, 15

International Nuclear Information System (INIS)

Ouchi, Tamon; Tsuji, Hideo.

1983-01-01

The patients exposed to the atomic bomb radiation in Hiroshima area came to Beppu Spa to have hot spring therapy. During the fiscal year of 1982 (April, 1982, to March, 1983), 3972 persons came to the hot spring sanatorium, and 586 patients (14.8 %) received physical examination. Among them, 473 patients (80.7 %) were exposed to the atomic bomb radiation on August 6, 1945, or entered in the city of Hiroshima by August 20, 1945, according to the official notebook issued by the government. Physical examination was performed twice a week during their stay, and more than 53.5 % of the patients were older than 70, and the oldest was 93 years old. Blood pressure was measured when the patients came in and went out, and hypertensive patients were asked to observe the rule of treatment strictly. The complaints of the patients which brought them to the hot spring were mostly pain in bodies and lower extremities, and hypertension, common cold syndrome, diabetes and constipation. Patients took hot spring bath 2 - 3 times daily, and many patients had microwave and low frequency wave treatment. Soaking in a bath (containing 1.4 mg of cupric sulfate and 11.4 mg of zinc sulfate per liter) was practiced by diabetic patients. The therapeutic effects were difficult to judge because the period of stay of the most patients was about 10 days, but in most of them, subjective symptoms were relieved when they left the sanatorium. (Yamashita, S.)

LINEAMENTS MAP OF TURKEY FROM LANDSAT IMAGERY AND SELECTING TARGET AREAS FOR MINERAL EXPLORATION, RELATIONSHIP OF REGIONAL LINEAMENTS TO EARTHQUAKE EPICENTERS, MINERAL WATERS AND HOT SPRINGS

Directory of Open Access Journals (Sweden)

İsmail HENDEN

1984-06-01

Full Text Available Landsat coverage countrywide have been interpreted and a map of lineaments has been prepared. Circular features which are the surface expressions of deep or near   intrusions have  been  carefully mapped in order to investigate their relationship (if any to known mineralizations. From the outset it was postulated that the miner- alizations  are    located at the intersections of lineaments, especially in the vicinity of circular features.  To  test this hypo- thesis known mineralizations were placed on this map. It is noted that the metallic mineral deposits can be grouped into ten regions,  and out  of these,  two regions need to  be explored more intensively. In  some regions selected, locations of possible mineralizations were determined. Earthquake epicenters, mineral water sources and hot spring locations were placed on the  lineaments map. It is seen  that the hot springs and earthquake epicentres are located on regional fault systems.

Geological, geochemical, and geophysical survey of the geothermal resources at Hot Springs Bay Valley, Akutan Island, Alaska

Energy Technology Data Exchange (ETDEWEB)

Motyka, R.J.; Wescott, E.M.; Turner, D.L.; Swanson, S.E.; Romick, J.D.; Moorman, M.A.; Poreda, R.J.; Witte, W.; Petzinger, B.; Allely, R.D.

1985-01-01

An extensive survey was conducted of the geothermal resource potential of Hot Springs Bay Valley on Akutan Island. A topographic base map was constructed, geologic mapping, geophysical and geochemical surveys were conducted, and the thermal waters and fumarolic gases were analyzed for major and minor element species and stable isotope composition. (ACR)

Microbial diversity of acidic hot spring (kawah hujan B) in geothermal field of kamojang area, west java-indonesia.

Science.gov (United States)

Aditiawati, Pingkan; Yohandini, Heni; Madayanti, Fida; Akhmaloka

2009-01-01

Microbial communities in an acidic hot spring, namely Kawah Hujan B, at Kamojang geothermal field, West Java-Indonesia was examined using culture dependent and culture independent strategies. Chemical analysis of the hot spring water showed a characteristic of acidic-sulfate geothermal activity that contained high sulfate concentrations and low pH values (pH 1.8 to 1.9). Microbial community present in the spring was characterized by 16S rRNA gene combined with denaturing gradient gel electrophoresis (DGGE) analysis. The majority of the sequences recovered from culture-independent method were closely related to Crenarchaeota and Proteobacteria phyla. However, detail comparison among the member of Crenarchaeota showing some sequences variation compared to that the published data especially on the hypervariable and variable regions. In addition, the sequences did not belong to certain genus. Meanwhile, the 16S Rdna sequences from culture-dependent samples revealed mostly close to Firmicute and gamma Proteobacteria.

Vulcan Hot Springs known geothermal resource area: an environmental analysis

Energy Technology Data Exchange (ETDEWEB)

Spencer, S.G.; Russell, B.F. (eds.)

1979-09-01

The Vulcan Hot Springs known geothermal resource area (KGRA) is one of the more remote KGRAs in Idaho. The chemistry of Vulcan Hot Springs indicates a subsurface resource temperature of 147/sup 0/C, which may be high enough for power generation. An analysis of the limited data available on climate, meteorology, and air quality indicates few geothermal development concerns in these areas. The KGRA is located on the edge of the Idaho Batholith on a north-trending lineament which may be a factor in the presence of the hot springs. An occasional earthquake of magnitude 7 or greater may be expected in the region. Subsidence or elevation as a result of geothermal development in the KGRA do not appear to be of concern. Fragile granitic soils on steep slopes in the KGRA are unstable and may restrict development. The South fork of the Salmon River, the primary stream in the region, is an important salmon spawning grounds. Stolle Meadows, on the edge of the KGRA, is used as a wintering and calving area for elk, and access to the area is limited during this period. Socioeconomic and demographic surveys indicate that facilities and services will probably not be significantly impacted by development. Known heritage resources in the KGRA include two sites and the potential for additional cultural sites is significant.

Application of heat-flow techniques to geothermal energy exploration, Leach Hot Springs area, Grass Valley, Nevada

Energy Technology Data Exchange (ETDEWEB)

Sass, J.H.; Ziagos, J.P.; Wollenberg, H.A.; Munroe, R.J.; di Somma, D.E.; Lachenbruch, A.H.

1977-01-01

A total of 82 holes ranging in depth from 18 to 400 meters were drilled for thermal and hydrologic studies in a 200 km/sup 2/ area of Grass Valley, Nevada, near Leach Hot Springs. Outside the immediate area of Leach Hot Springs, heat flow ranges from 1 to 6.5 hfu with a mean of 2.4 hfu (1 hfu = 10/sup -6/ cal cm/sup 2/ s/sup -1/ = 41.8 mWm/sup -2/). Within 2 km of the springs, conductive heat flow ranges between 1.6 and more than 70 hfu averaging 13.6 hfu. Besides the conspicuous thermal anomaly associated with the hot springs, two additional anomalies were identified. One is associated with faults bounding the western margin of the Tobin Range near Panther Canyon, and the other is near the middle of Grass Valley about 5 km SSW of Leach Hot Springs. The mid-valley anomaly appears to be caused by hydrothermal circulation in a bedrock horst beneath about 375 meters of impermeable valley sediments. If the convective and conductive heat discharge within 2 km of the Leach Hot Springs is averaged over the entire hydrologic system (including areas of recharge), the combined heat flux from this part of Grass Valley is about 3 hfu, consistent with the average regional conductive heat flow in the Battle Mountain High. The hydrothermal system can be interpreted as being in a stationary stable phase sustained by high regional heat flow, and no localized crustal heat sources (other than hydrothermal convection to depths of a few kilometers) need be invoked to explain the existence of Leach Hot Springs.

Genome Sequence of a Novel Archaeal Rudivirus Recovered from a Mexican Hot Spring

DEFF Research Database (Denmark)

Servín-Garcidueñas, L; Peng, X; Garrett, R

2013-01-01

We report the consensus genome sequence of a novel GC-rich rudivirus, designated SMR1 (Sulfolobales Mexican rudivirus 1), assembled from a high-throughput sequenced environmental sample from a hot spring in Los Azufres National Park in western Mexico.......We report the consensus genome sequence of a novel GC-rich rudivirus, designated SMR1 (Sulfolobales Mexican rudivirus 1), assembled from a high-throughput sequenced environmental sample from a hot spring in Los Azufres National Park in western Mexico....

Biodiversity of thermophilic prokaryotes with hydrolytic activities in hot springs of Uzon Caldera, Kamchatka (Russia).

Science.gov (United States)

Kublanov, Ilya V; Perevalova, Anna A; Slobodkina, Galina B; Lebedinsky, Aleksander V; Bidzhieva, Salima K; Kolganova, Tatyana V; Kaliberda, Elena N; Rumsh, Lev D; Haertlé, Thomas; Bonch-Osmolovskaya, Elizaveta A

2009-01-01

Samples of water from the hot springs of Uzon Caldera with temperatures from 68 to 87 degrees C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or alpha- or beta-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric substrates were obtained. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments obtained after PCR with Bacteria-specific primers showed that the bacterial communities developing on carbohydrates included the genera Caldicellulosiruptor and Dictyoglomus and that those developing on proteins contained members of the Thermotogales order. DGGE analysis performed after PCR with Archaea- and Crenarchaeota-specific primers showed that archaea related to uncultured environmental clones, particularly those of the Crenarchaeota phylum, were present in both carbohydrate- and protein-degrading communities. Five isolates obtained from in situ enrichments or corresponding natural samples of water and sediments represented the bacterial genera Dictyoglomus and Caldanaerobacter as well as new archaea of the Crenarchaeota phylum. Thus, in situ enrichment and consequent isolation showed the diversity of thermophilic prokaryotes competing for biopolymers in microbial communities of terrestrial hot springs.

Studying Prokaryotic Communities in Iron Depositing Hot Springs (IDHS): Implication for Early Mars Habitability

Science.gov (United States)

Sarkisova, S. A.; Tringe, S. G.; Thomas-Keprta, K. L.; Allen, C. c.; Garrison, D. H.; McKay, David S.; Brown, I. I.

2010-01-01

We speculate that both external and intracellular iron precipitate in iron-tolerant CB might be involved in oxidative stress suppression shown by [9]. Significant differences are apparent between a set of proteins involved in the maintenance of Fe homeostasis and oxidative stress protection in iron-tolerant and fresh-water and marine CB. Correspondingly, these properties may help to make iron-tolerant CB as dominant organisms in IDHS and probably on early Earth and Mars. Further comparative analyses of hot springs metagenomes and the genomes of iron-tolerant microbes versus fresh-water/marine ones may point out to different habitable zones on early Mars.

Groundwater flow cycling between a submarine spring and an inland fresh water spring.

Science.gov (United States)

Davis, J Hal; Verdi, Richard

2014-01-01

Spring Creek Springs and Wakulla Springs are large first magnitude springs that derive water from the Upper Floridan Aquifer. The submarine Spring Creek Springs are located in a marine estuary and Wakulla Springs are located 18 km inland. Wakulla Springs has had a consistent increase in flow from the 1930s to the present. This increase is probably due to the rising sea level, which puts additional pressure head on the submarine Spring Creek Springs, reducing its fresh water flow and increasing flows in Wakulla Springs. To improve understanding of the complex relations between these springs, flow and salinity data were collected from June 25, 2007 to June 30, 2010. The flow in Spring Creek Springs was most sensitive to rainfall and salt water intrusion, and the flow in Wakulla Springs was most sensitive to rainfall and the flow in Spring Creek Springs. Flows from the springs were found to be connected, and composed of three repeating phases in a karst spring flow cycle: Phase 1 occurred during low rainfall periods and was characterized by salt water backflow into the Spring Creek Springs caves. The higher density salt water blocked fresh water flow and resulted in a higher equivalent fresh water head in Spring Creek Springs than in Wakulla Springs. The blocked fresh water was diverted to Wakulla Springs, approximately doubling its flow. Phase 2 occurred when heavy rainfall resulted in temporarily high creek flows to nearby sinkholes that purged the salt water from the Spring Creek Springs caves. Phase 3 occurred after streams returned to base flow. The Spring Creek Springs caves retained a lower equivalent fresh water head than Wakulla Springs, causing them to flow large amounts of fresh water while Wakulla Springs flow was reduced by about half. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.

10 CFR 431.102 - Definitions concerning commercial water heaters, hot water supply boilers, and unfired hot water...

Science.gov (United States)

2010-01-01

... supply boilers, and unfired hot water storage tanks. 431.102 Section 431.102 Energy DEPARTMENT OF ENERGY... Water Heaters, Hot Water Supply Boilers and Unfired Hot Water Storage Tanks § 431.102 Definitions concerning commercial water heaters, hot water supply boilers, and unfired hot water storage tanks. The...

Portrait of a Geothermal Spring, Hunter’s Hot Springs, Oregon

Directory of Open Access Journals (Sweden)

Richard W. Castenholz

2015-01-01

Full Text Available Although alkaline Hunter’s Hot Springs in southeastern Oregon has been studied extensively for over 40 years, most of these studies and the subsequent publications were before the advent of molecular methods. However, there are many field observations and laboratory experiments that reveal the major aspects of the phototrophic species composition within various physical and chemical gradients of these springs. Relatively constant temperature boundaries demark the upper boundary of the unicellular cyanobacterium, Synechococcus at 73–74 °C (the world-wide upper limit for photosynthesis, and 68–70 °C the upper limit for Chloroflexus. The upper limit for the cover of the filamentous cyanobacterium, Geitlerinema (Oscillatoria is at 54–55 °C, and the in situ lower limit at 47–48 °C for all three of these phototrophs due to the upper temperature limit for the grazing ostracod, Thermopsis. The in situ upper limit for the cyanobacteria Pleurocapsa and Calothrix is at ~47–48 °C, which are more grazer-resistant and grazer dependent. All of these demarcations are easily visible in the field. In addition, there is a biosulfide production in some sections of the springs that have a large impact on the microbiology. Most of the temperature and chemical limits have been explained by field and laboratory experiments.

Portrait of a Geothermal Spring, Hunter’s Hot Springs, Oregon

Science.gov (United States)

Castenholz, Richard W.

2015-01-01

Although alkaline Hunter’s Hot Springs in southeastern Oregon has been studied extensively for over 40 years, most of these studies and the subsequent publications were before the advent of molecular methods. However, there are many field observations and laboratory experiments that reveal the major aspects of the phototrophic species composition within various physical and chemical gradients of these springs. Relatively constant temperature boundaries demark the upper boundary of the unicellular cyanobacterium, Synechococcus at 73–74 °C (the world-wide upper limit for photosynthesis), and 68–70 °C the upper limit for Chloroflexus. The upper limit for the cover of the filamentous cyanobacterium, Geitlerinema (Oscillatoria) is at 54–55 °C, and the in situ lower limit at 47–48 °C for all three of these phototrophs due to the upper temperature limit for the grazing ostracod, Thermopsis. The in situ upper limit for the cyanobacteria Pleurocapsa and Calothrix is at ~47–48 °C, which are more grazer-resistant and grazer dependent. All of these demarcations are easily visible in the field. In addition, there is a biosulfide production in some sections of the springs that have a large impact on the microbiology. Most of the temperature and chemical limits have been explained by field and laboratory experiments. PMID:25633225

Phototrophy in Mildly Acidic Hot Spring Ecosystems

Science.gov (United States)

Fecteau, K.; Boyd, E. S.; Shock, E.

2014-12-01

Microbial light-driven reduction of carbon in continental hydrothermal ecosystems is restricted to environments at temperatures less than 73 °C. In circumneutral and alkaline systems bacterial phototrophs (cyanobacteria and anoxygenic phototrophs) are suggested to be principally responsible for this activity whereas algal (i.e., eukaryotic) phototrophs are thought to be responsible for this activity in acidic systems. In Yellowstone National Park numerous examples of phototrophic microbial communities exist at high and low pH, while hot springs with intermediate pH (values 3-5) are rare and commonly dilute. It is thought that the transition from algal photosynthesis to bacterial photosynthesis occurs within this pH range. To test this hypothesis, we sequenced bacterial and eukaryal small subunit ribosomal RNA genes, analyzed pigments, and performed comprehensive geochemical measurements from 12 hot springs within this pH realm. At all sites, the largest phototrophic population was either comprised of Cyanobacteria or affiliated with the algal order Cyanidiales, which are ubiquitous in acidic springs, yet abundant sequences of both lineages were present in 8 of the 12 sites. Nevertheless, some of these samples exceeded the known temperature limit of the algae (56 °C), suggesting that these populations are dead or inactive. Indeed, one site yielded evidence for a large Cyanidiales population as the only phototrophs present, yet an experiment at the time of sampling failed to demonstrate light-driven carbon fixation, and analysis of extracted pigments showed a large amount of the chlorophyll degradation product pheophorbide a and very little intact chlorophyll, indicating photosynthesis occurred at this site when conditions were different. Our observations illustrate the dynamic nature of these systems that may be transiently conducive to photosynthesis, which may open niches for phototrophs of both domains and likely played a role in the evolution of photosynthesis.

Gas geochemistry of the hot spring in the Litang fault zone, Southeast Tibetan Plateau

International Nuclear Information System (INIS)

Zhou, Xiaocheng; Liu, Lei; Chen, Zhi; Cui, Yueju; Du, Jianguo

2017-01-01

The southeast Tibetan Plateau is a region with high level seismic activity and strong hydrothermal activity. Several large (7.5 > M > 7) historical earthquakes have occurred in the Litang fault zone (LFZ), eastern Tibetan Plateau since 1700. Litang Ms 5.1 earthquake occurred On Sept 23, 2016, indicating the reactivation of the LFZ. This study was undertaken to elucidate spatial-temporal variations of the hot spring gas geochemistry along the LFZ from Jun 2010 to April 2016. The chemical components, He, Ne and C isotropic ratios of bubbling gas samples taken from 18 hot springs along LFZ were investigated. Helium isotope ratios ( 3 He/ 4 He) measured in hot springs varied from 0.06 to 0.93 Ra (Ra = air 3 He/ 4 He = 1.39 × 10 −6 ), with mantle-derivd He up to 11.1% in the LFZ (assuming R/Ra = 8 for mantle) indicated the fault was a crustal-scale feature that acts as a conduit for deep fluid from the mantle. CO 2 concentrations of the majority of hot spring gas samples were ≥80 vol%, CO 2 / 3 He ratios varied from 1.4 to 929.5 × 10 10 , and δ 13 C CO2 values varied from −19.2‰ to −2.3‰ (vs. PDB). The proportions of mantle-derived CO 2 varied from 0 to 1.8%. Crustal marine limestone was the major contributor (>75%) to the carbon inventory of the majority of hot spring gas samples. Before Litang Ms 5.1 earthquake, the 3 He/ 4 He ratios obviously increased in the Heni spring from May 2013 to Apr 2016. The geographical distribution of the mantle-derivd He decreased from east to west along 30°N in the southeast Tibetan Plateau relative to a corresponding increase in the radiogenic component. The gas geochemical data suggested that the upwelling mantle fluids into the crust play an important role in seismic activity in the strike-slip faults along 30°N in the southeast Tibetan Plateau. - Highlights: • Gas geochemistry of hot springs along Litang fault, Southeast Tibetan Plateau were surveyed. • Mantle-derived He decreased from east to

Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia)▿ †

OpenAIRE

Kublanov, Ilya V.; Perevalova, Anna A.; Slobodkina, Galina B.; Lebedinsky, Aleksander V.; Bidzhieva, Salima K.; Kolganova, Tatyana V.; Kaliberda, Elena N.; Rumsh, Lev D.; Haertlé, Thomas; Bonch-Osmolovskaya, Elizaveta A.

2008-01-01

Samples of water from the hot springs of Uzon Caldera with temperatures from 68 to 87°C and pHs of 4.1 to 7.0, supplemented with proteinaceous (albumin, casein, or α- or β-keratin) or carbohydrate (cellulose, carboxymethyl cellulose, chitin, or agarose) biological polymers, were filled with thermal water and incubated at the same sites, with the contents of the tubes freely accessible to the hydrothermal fluid. As a result, several enrichment cultures growing in situ on different polymeric su...

Disaggregating Hot Water Use and Predicting Hot Water Waste in Five Test Homes

Energy Technology Data Exchange (ETDEWEB)

Henderson, H.; Wade, J.

2014-04-01

While it is important to make the equipment (or 'plant') in a residential hot water system more efficient, the hot water distribution system also affects overall system performance and energy use. Energy wasted in heating water that is not used is estimated to be on the order of 10 to 30 percent of total domestic hot water (DHW) energy use. This field monitoring project installed temperature sensors on the distribution piping (on trunks and near fixtures) and programmed a data logger to collect data at 5 second intervals whenever there was a hot water draw. This data was used to assign hot water draws to specific end uses in the home as well as to determine the portion of each hot water that was deemed useful (i.e., above a temperature threshold at the fixture). Five houses near Syracuse NY were monitored. Overall, the procedures to assign water draws to each end use were able to successfully assign about 50% of the water draws, but these assigned draws accounted for about 95% of the total hot water use in each home. The amount of hot water deemed as useful ranged from low of 75% at one house to a high of 91% in another. At three of the houses, new water heaters and distribution improvements were implemented during the monitoring period and the impact of these improvements on hot water use and delivery efficiency were evaluated.

Disaggregating Hot Water Use and Predicting Hot Water Waste in Five Test Homes

Energy Technology Data Exchange (ETDEWEB)

Henderson, Hugh [ARIES Collaborative, New York, NY (United States); Wade, Jeremy [ARIES Collaborative, New York, NY (United States)

2014-04-01

While it is important to make the equipment (or "plant") in a residential hot water system more efficient, the hot water distribution system also affects overall system performance and energy use. Energy wasted in heating water that is not used is estimated to be on the order of 10%-30% of total domestic hot water (DHW) energy use. This field monitoring project installed temperature sensors on the distribution piping (on trunks and near fixtures) in five houses near Syracuse, NY, and programmed a data logger to collect data at 5 second intervals whenever there was a hot water draw. This data was used to assign hot water draws to specific end uses in the home as well as to determine the portion of each hot water that was deemed useful (i.e., above a temperature threshold at the fixture). Overall, the procedures to assign water draws to each end use were able to successfully assign about 50% of the water draws, but these assigned draws accounted for about 95% of the total hot water use in each home. The amount of hot water deemed as useful ranged from low of 75% at one house to a high of 91% in another. At three of the houses, new water heaters and distribution improvements were implemented during the monitoring period and the impact of these improvements on hot water use and delivery efficiency were evaluated.

Metagenomic Analysis of Hot Springs in Central India Reveals Hydrocarbon Degrading Thermophiles and Pathways Essential for Survival in Extreme Environments

Science.gov (United States)

Saxena, Rituja; Dhakan, Darshan B.; Mittal, Parul; Waiker, Prashant; Chowdhury, Anirban; Ghatak, Arundhuti; Sharma, Vineet K.

2017-01-01

Extreme ecosystems such as hot springs are of great interest as a source of novel extremophilic species, enzymes, metabolic functions for survival and biotechnological products. India harbors hundreds of hot springs, the majority of which are not yet explored and require comprehensive studies to unravel their unknown and untapped phylogenetic and functional diversity. The aim of this study was to perform a large-scale metagenomic analysis of three major hot springs located in central India namely, Badi Anhoni, Chhoti Anhoni, and Tattapani at two geographically distinct regions (Anhoni and Tattapani), to uncover the resident microbial community and their metabolic traits. Samples were collected from seven distinct sites of the three hot spring locations with temperature ranging from 43.5 to 98°C. The 16S rRNA gene amplicon sequencing of V3 hypervariable region and shotgun metagenome sequencing uncovered a unique taxonomic and metabolic diversity of the resident thermophilic microbial community in these hot springs. Genes associated with hydrocarbon degradation pathways, such as benzoate, xylene, toluene, and benzene were observed to be abundant in the Anhoni hot springs (43.5–55°C), dominated by Pseudomonas stutzeri and Acidovorax sp., suggesting the presence of chemoorganotrophic thermophilic community with the ability to utilize complex hydrocarbons as a source of energy. A high abundance of genes belonging to methane metabolism pathway was observed at Chhoti Anhoni hot spring, where methane is reported to constitute >80% of all the emitted gases, which was marked by the high abundance of Methylococcus capsulatus. The Tattapani hot spring, with a high-temperature range (61.5–98°C), displayed a lower microbial diversity and was primarily dominated by a nitrate-reducing archaeal species Pyrobaculum aerophilum. A higher abundance of cell metabolism pathways essential for the microbial survival in extreme conditions was observed at Tattapani. Taken together

Fiscal 1999 geothermal energy development promotion survey. Report on survey of introduction of techniques for predicting impact on hot springs; 1999 nendo chinetsu kaihatsu sokushin chosa hokokusho. Onsen eikyo yosoku shuho donyu chosa

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-08-01

In an effort to find guidelines on how to proceed with geothermal energy development so that it may coexist with hot springs, investigations are conducted into cases of impacts on hot springs imposed by geothermal energy development activities. An impact is judged to exist when geothermal development results in a decrease or depletion of pumped or spontaneously welling hot spring water, change in the concentration of dissolved chemical ingredients, fall in water temperature, or in an increase in the amount of discharged steam. Keyword-aided retrieval of data from databases is performed, and geothermal magazines are referred to for information. There are articles reporting impacts imposed by geothermal development on hot springs in the Palinpinon area (Philippines) and 12 others and in the Corwin Springs area (U.S.) and 13 others. These articles carrying outlines and impacts of geothermal development are collected, put in order, and analyzed. Cases in which such impacts are found to exist are categorized into four groups and, in each group, episodes are differentiated from each other by the type of mechanism linking the aquifer and the reservoir which is the object of development. (NEDO)

Diversity of thermophilic archaeal isolates from hot springs in Japan

Science.gov (United States)

Itoh, Takashi; Yoshikawa, Naoto; Takashina, Tomonori

2005-09-01

In the light of the significance of extremophiles as model organisms to access possible extraterrestiral life, we provide a short review of the systematics of thermophilic Archaea, and introduce our exploratory research of novel thermophilic Archaea from hot springs in Japan. Up to date, we have isolated 162 strains of the thermophilic Archaea from hot springs in Japan by the enrichment method or the most probable number/PCR method, and the 16S rRNA gene sequences were determined to reveal their phylogenetic diversity. The sequence comparison illustrated that the isolates belonged to the orders Sulfolobales (117 isolates) , Thermoproteales (29 isolates), Desulfurococcales (8 isolates) and Thermoplasmatales (8 isolates), and there were six separate lineages representing new genera, and at least seven new species as predicted by the phylogenetic distance to known species. The collection of isolates not only included novel taxa but would give some implication for a necessity to reevaluate the current taxonomy of the thermophilic Archaea.

Functional genes and thermophilic microorganisms responsible for arsenite oxidation from the shallow sediment of an untraversed hot spring outlet.

Science.gov (United States)

Yang, Ye; Mu, Yao; Zeng, Xian-Chun; Wu, Weiwei; Yuan, Jie; Liu, Yichen; Guoji, E; Luo, Feng; Chen, Xiaoming; Li, Hao; Wang, Jianing

2017-05-01

Hot Springs have unique geochemical features. Microorganisms-mediated arsenite oxidation is one of the major biogeochemical processes occurred in some hot springs. This study aimed to understand the diversities of genes and microorganisms involved in arsenite oxidation from the outlet of an untraversed hot spring located at an altitude of 4226 m. Microcosm assay indicated that the microbial community from the hot spring was able to efficiently oxidize As(III) using glucose, lactic acid, yeast extract or sodium bicarbonate as the sole carbon source. The microbial community contained 7 phyla of microorganisms, of which Proteobacteria and Firmicutes are largely dominant; this composition is unique and differs significantly from those of other described hot springs. Twenty one novel arsenite oxidase genes were identified from the samples, which are affiliated with the arsenite oxidase families of α-Proteobacteria, β-Proteobacteria or Archaea; this highlights the high diversity of the arsenite-oxidizing microorganisms from the hot spring. A cultivable arsenite-oxidizer Chelatococcu sp. GHS311 was also isolated from the sample using enrichment technique. It can completely convert 75.0 mg/L As(III) into As(V) in 18 days at 45 °C. The arsenite oxidase of GHS311 shares the maximal sequence identity (84.7%) to that of Hydrogenophaga sp. CL3, a non-thermotolerant bacterium. At the temperature lower than 30 °C or higher than 65 °C, the growth of this strain was completely inhibited. These data help us to better understand the diversity and functional features of the thermophilic arsenite-oxidizing microorganisms from hot springs.

Regulatory, Land Ownership, and Water Availability Factors for a Magma Well: Long Valley Caldera and Coso Hot Springs, California

Energy Technology Data Exchange (ETDEWEB)

Blackett, Robert

1985-09-01

The U.S. Department of Energy is currently engaged in a program to demonstrate the engineering feasibility of extracting thermal energy from high-level molten magma bodies. The program is being carried out under the direction of Sandia National Laboratories where a number of individual projects support the overall program. The existing program elements include (1) high-temperature materials compatibility testing; (2) studies of properties of melts of various compositions; and (3) the investigation of the economics of a magma energy extraction system. Another element of the program is being conducted with the cooperation of the U.S. Geological Survey, and involves locating and outlining magma bodies at selected sites using various geophysical techniques. The ultimate goal here will be to define the limits of a magma body as a drilling target. During an earlier phase of the program, more than twenty candidate study sites considered were evaluated based upon: (1) the likelihood of the presence of a shallow magma chamber, (2) the accessibility of the site, and (3) physical and institutional constraints associated with each site with respect to performing long-term experiments. From these early phase activities, the number of candidate sites were eventually narrowed to just 2. The sites currently under consideration are Coso Hot Springs and the Long Valley caldera (Figure 1). This report describes certain attributes of these sites in order to help identify potential problems related to: (1) state and federal regulations pertaining to geothermal development; (2) land ownership; and (3) water resource availability. The information sources used in this study were mainly maps, publications, and informative documents gathered from the California Division of Oil and Gas and the U.S. Department of the Interior. Environmental studies completed for the entire Long Valley caldera study area, and for portions of the Coso Hot Springs study area were also used for reference.

Diversity of Cultured Thermophilic Anaerobes in Hot Springs of Yunnan Province, China

Science.gov (United States)

Lin, L.; Lu, Y.; Dong, X.; Liu, X.; Wei, Y.; Ji, X.; Zhang, C.

2010-12-01

Thermophilic anaerobes including Archaea and Bacteria refer to those growing optimally at temperatures above 50°C and do not use oxygen as the terminal electron acceptor for growth. Study on thermophilic anaerobes will help to understand how life thrives under extreme conditions. Meanwhile thermophilic anaerobes are of importance in potential application and development of thermophilic biotechnology. We have surveyed culturable thermophilic anaerobes in hot springs (pH6.5-7.5; 70 - 94°C) in Rehai of Tengchong, Bangnazhang of Longlin, Eryuan of Dali,Yunnan, China. 50 strains in total were cultured from the hot springs water using Hungate anaerobic technique, and 30 strains were selected based on phenotypic diversity for analysis of 16S rDNA sequences. Phylogenetic analysis showed that 28 strains belonged to the members of five genera: Caldanaerobacter, Calaramator, Thermoanaerobacter, Dictyoglomus and Fervidobacterium, which formed five branches on the phylogenetic tree. Besides, 2 strains of methanogenic archaea were obtained. The majority of the isolates were the known species, however, seven strains were identified as novel species affiliated to the five genera based on the lower 16S rDNA sequence similarities (less than 93 - 97%) with the described species. This work would provide the future study on their diversity, distribution among different regions and the potential application of thermophilic enzyme. Supported by State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences(SKLMR-080605)and the Foundation of State Natural Science (30660009, 30960022, 31081220175).

Hot Springs-Garrison Fiber Optic Project

International Nuclear Information System (INIS)

1994-10-01

Bonneville Power Administration (BPA) is proposing to upgrade its operational telecommunications system between the Hot Springs Substation and the Garrison Substation using a fiber optic system. The project would primarily involve installing 190 kilometers (120 miles) of fiber optic cable on existing transmission structures and installing new fiber optic equipment in BPA's substation yards and control houses. BPA prepared an environmental assessment (EA) evaluating the proposed action. This EA was published in October 1994. The EA identifies a number of minor impacts that might occur as a result of the proposed action, as well as some recommended mitigation measures. This Mitigation Action Plan (MAP) identifies specific measures to avoid, minimize, or compensate for impacts identified in the EA

Hot Springs-Garrison Fiber Optic Project

Energy Technology Data Exchange (ETDEWEB)

1994-10-01

Bonneville Power Administration (BPA) is proposing to upgrade its operational telecommunications system between the Hot Springs Substation and the Garrison Substation using a fiber optic system. The project would primarily involve installing 190 kilometers (120 miles) of fiber optic cable on existing transmission structures and installing new fiber optic equipment in BPA`s substation yards and control houses. BPA prepared an environmental assessment (EA) evaluating the proposed action. This EA was published in October 1994. The EA identifies a number of minor impacts that might occur as a result of the proposed action, as well as some recommended mitigation measures. This Mitigation Action Plan (MAP) identifies specific measures to avoid, minimize, or compensate for impacts identified in the EA.

Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing.

Science.gov (United States)

Chan, Chia Sing; Chan, Kok-Gan; Tay, Yea-Ling; Chua, Yi-Heng; Goh, Kian Mau

2015-01-01

The Sungai Klah (SK) hot spring is the second hottest geothermal spring in Malaysia. This hot spring is a shallow, 150-m-long, fast-flowing stream, with temperatures varying from 50 to 110°C and a pH range of 7.0-9.0. Hidden within a wooded area, the SK hot spring is continually fed by plant litter, resulting in a relatively high degree of total organic content (TOC). In this study, a sample taken from the middle of the stream was analyzed at the 16S rRNA V3-V4 region by amplicon metagenome sequencing. Over 35 phyla were detected by analyzing the 16S rRNA data. Firmicutes and Proteobacteria represented approximately 57% of the microbiome. Approximately 70% of the detected thermophiles were strict anaerobes; however, Hydrogenobacter spp., obligate chemolithotrophic thermophiles, represented one of the major taxa. Several thermophilic photosynthetic microorganisms and acidothermophiles were also detected. Most of the phyla identified by 16S rRNA were also found using the shotgun metagenome approaches. The carbon, sulfur, and nitrogen metabolism within the SK hot spring community were evaluated by shotgun metagenome sequencing, and the data revealed diversity in terms of metabolic activity and dynamics. This hot spring has a rich diversified phylogenetic community partly due to its natural environment (plant litter, high TOC, and a shallow stream) and geochemical parameters (broad temperature and pH range). It is speculated that symbiotic relationships occur between the members of the community.

Site-specific analysis of hybrid geothermal/fossil power plants. Volume One. Roosevelt Hot Springs KGRA

Energy Technology Data Exchange (ETDEWEB)

1977-06-01

The economics of a particular hybrid plant must be evaluated with respect to a specific site. This volume focuses on the Roosevelt Hot Springs KGRA. The temperature, pressure, and flow rate data given suggests the site deserves serious consideration for a hybrid plant. Key siting considerations which must be addressed before an economic judgment can be attempted are presented as follows: the availability, quality, and cost of coal; the availability of water; and the availability of transmission. Seismological and climate factors are presented. (MHR)

Exposure to Particle Matters and Hazardous Volatile Organic Compounds in Selected Hot Spring Hotels in Guangdong, China

Directory of Open Access Journals (Sweden)

Qiusheng He

2016-04-01

Full Text Available In Guangdong province, many hot springs were exploited and developed into popular places for tourist. In addition, hotels have been set up near hot spring sites to attract people, including local citizens, to spend their spare time inside these so-called “spring hotels”. In our study, indoor air quality was investigated in four hot spring hotels in Guangdong province, China. Measured indoor pollutants included CO2, CO, PM10, PM2.5 and Volatile Organic Compounds (VOCs. As the result show, high concentrations of carbon dioxide might be attributed to poor ventilation; and the variations of indoor PM10, PM2.5 concentrations were related to occupants’ activities. Alpha-pinene and toluene were the most common VOC species in the hot spring hotels other than monocyclic aromatic hydrocarbons like Benzene, Toluene, Ethylbenzene and Xylenes (BTEX, which were at medium levels among the reported indoor pollutants. High cancer risk of benzene in the newly decorated rooms should be seriously taken into consideration in the future. Indoor to Outdoor air concentration ratios (I/O for CO2 and VOCs were higher than 1, indicating their strong indoor sources. Negative correlations were found between indoor CO2 and all the other compounds, and VOCs were shown to be significantly correlated (p < 0.01 to each other, including aromatic hydrocarbons and mono-terpenes. For indoor and outdoor air compounds, correlation coefficients among all compounds did not show a significant correlation, which indicated that these pollutants had different sources. Principal components analysis by SPSS showed that indoor materials, inhabitants’ activities and respiration, cleaning products and outdoor sources were the main sources of indoor detected pollutants in hot spring hotels.

The cyanobacterium Mastigocladus fulfills the nitrogen demand of a terrestrial hot spring microbial mat.

Science.gov (United States)

Estrella Alcamán, María; Fernandez, Camila; Delgado, Antonio; Bergman, Birgitta; Díez, Beatriz

2015-10-01

Cyanobacteria from Subsection V (Stigonematales) are important components of microbial mats in non-acidic terrestrial hot springs. Despite their diazotrophic nature (N2 fixers), their impact on the nitrogen cycle in such extreme ecosystems remains unknown. Here, we surveyed the identity and activity of diazotrophic cyanobacteria in the neutral hot spring of Porcelana (Northern Patagonia, Chile) during 2009 and 2011-2013. We used 16S rRNA and the nifH gene to analyze the distribution and diversity of diazotrophic cyanobacteria. Our results demonstrate the dominance of the heterocystous genus Mastigocladus (Stigonematales) along the entire temperature gradient of the hot spring (69-38 °C). In situ nitrogenase activity (acetylene reduction), nitrogen fixation rates (cellular uptake of (15)N2) and nifH transcription levels in the microbial mats showed that nitrogen fixation and nifH mRNA expression were light-dependent. Nitrogen fixation activities were detected at temperatures ranging from 58 °C to 46 °C, with maximum daily rates of 600 nmol C2H4 cm(-2) per day and 94.1 nmol N cm(-2) per day. These activity patterns strongly suggest a heterocystous cyanobacterial origin and reveal a correlation between nitrogenase activity and nifH gene expression during diurnal cycles in thermal microbial mats. N and C fixation in the mats contributed ~3 g N m(-2) per year and 27 g C m(-2) per year, suggesting that these vital demands are fully met by the diazotrophic and photoautotrophic capacities of the cyanobacteria in the Porcelana hot spring.

Archaeal and bacterial diversity in two hot spring microbial mats from a geothermal region in Romania.

Science.gov (United States)

Coman, Cristian; Drugă, Bogdan; Hegedus, Adriana; Sicora, Cosmin; Dragoş, Nicolae

2013-05-01

The diversity of archaea and bacteria was investigated in two slightly alkaline, mesophilic hot springs from the Western Plain of Romania. Phylogenetic analysis showed a low diversity of Archaea, only three Euryarchaeota taxa being detected: Methanomethylovorans thermophila, Methanomassiliicoccus luminyensis and Methanococcus aeolicus. Twelve major bacterial groups were identified, both springs being dominated by Cyanobacteria, Chloroflexi and Proteobacteria. While at the phylum/class-level the microbial mats share a similar biodiversity; at the species level the geothermal springs investigated seem to be colonized by specific consortia. The dominant taxa were filamentous heterocyst-containing Fischerella, at 45 °C and non-heterocyst Leptolyngbya and Geitlerinema, at 55 °C. Other bacterial taxa (Thauera sp., Methyloversatilis universalis, Pannonibacter phragmitetus, Polymorphum gilvum, Metallibacterium sp. and Spartobacteria) were observed for the first time in association with a geothermal habitat. Based on their bacterial diversity the two mats were clustered together with other similar habitats from Europe and part of Asia, most likely the water temperature playing a major role in the formation of specific microbial communities that colonize the investigated thermal springs.

CRISPR Spacer Arrays for Detection of Viral Signatures from Acidic Hot Springs

Science.gov (United States)

Snyder, J. C.; Bateson, M. M.; Suciu, D.; Young, M. J.

2010-04-01

Viruses are the most abundant life-like entities on the planet Earth. Using CRISPR spacer sequences, we have developed a microarray-based approach to detecting viral signatures in the acidic hot springs of Yellowstone.

Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing

Directory of Open Access Journals (Sweden)

Chia Sing eChan

2015-03-01

Full Text Available The Sungai Klah (SK hot spring is the second hottest geothermal spring in Malaysia. This hot spring is a shallow, 150-meter-long, fast-flowing stream, with temperatures varying from 50 to 110°C and a pH range of 7.0 to 9.0. Hidden within a wooded area, the SK hot spring is continually fed by plant litter, resulting in a relatively high degree of total organic content (TOC. In this study, a sample taken from the middle of the stream was analyzed at the 16S rRNA V3−V4 region by amplicon metagenome sequencing. Over 35 phyla were detected by analyzing the 16S rRNA data. Firmicutes and Proteobacteria represented approximately 57% of the microbiome. Approximately 70% of the detected thermophiles were strict anaerobes; however, Hydrogenobacter spp., obligate chemolithotrophic thermophiles, represented one of the major taxa. Several thermophilic photosynthetic microorganisms and acidothermophiles were also detected. Most of the phyla identified by 16S rRNA were also found using the shotgun metagenome approaches. The carbon, sulfur, and nitrogen metabolism within the SK hot spring community were evaluated by shotgun metagenome sequencing, and the data revealed diversity in terms of metabolic activity and dynamics. This hot spring has a rich diversified phylogenetic community partly due to its natural environment (plant litter, high TOC, and a shallow stream and geochemical parameters (broad temperature and pH range. It is speculated that symbiotic relationships occur between the members of the community.

Penentuan Strategi Pengembangan Pariwisata Menggunakan Metode Analisis Swot (Studi Pada Pemandian Air Panas atau Hot Spring di Kelurahan Siogung-Ogung Kecamatan Pangururan Kabupaten Samosir)

OpenAIRE

Napitu, Kartini Indayati

2016-01-01

Hot spring is a tourist attraction that has the potential to attract more visitors if developed with good strategy. Therefore , researchers interested in studying how to develop the tourist hot spring and strategies that can be done. This study aims to , first to analyze the factors that the strengths, weaknesses, opportunities and threats to tourism Hot Spring in Pangururan Samosir. Second, determine the right marketing strategy in tourism Hot Spring is based on the analysis of strengths , w...

Biophysical model of prokaryotic diversity in geothermal hot springs.

Science.gov (United States)

Klales, Anna; Duncan, James; Nett, Elizabeth Janus; Kane, Suzanne Amador

2012-02-01

Recent studies of photosynthetic bacteria living in geothermal hot spring environments have revealed surprisingly complex ecosystems with an unexpected level of genetic diversity. One case of particular interest involves the distribution along hot spring thermal gradients of genetically distinct bacterial strains that differ in their preferred temperatures for reproduction and photosynthesis. In such systems, a single variable, temperature, defines the relevant environmental variation. In spite of this, each region along the thermal gradient exhibits multiple strains of photosynthetic bacteria adapted to several distinct thermal optima, rather than a single thermal strain adapted to the local environmental temperature. Here we analyze microbiology data from several ecological studies to show that the thermal distribution data exhibit several universal features independent of location and specific bacterial strain. These include the distribution of optimal temperatures of different thermal strains and the functional dependence of the net population density on temperature. We present a simple population dynamics model of these systems that is highly constrained by biophysical data and by physical features of the environment. This model can explain in detail the observed thermal population distributions, as well as certain features of population dynamics observed in laboratory studies of the same organisms. © 2012 American Physical Society

Thermostable 𝜶-Amylase Activity from Thermophilic Bacteria Isolated from Bora Hot Spring, Central Sulawesi

Science.gov (United States)

Gazali, F. M.; Suwastika, I. N.

2018-03-01

α-Amylase is one of the most important enzyme in biotechnology field, especially in industrial application. Thermostability of α-Amylase produced by thermophilic bacteria improves industrial process of starch degradation in starch industry. The present study were concerned to the characterization of α-Amylase activity from indigenous thermophilic bacteria isolated from Bora hot spring, Central Sulawesi. There were 18 isolates which had successfully isolated from 90°C sediment samples of Bora hot spring and 13 of them showed amylolytic activity. The α-Amylase activity was measured qualitatively at starch agar and quantitatively based on DNS (3,5-Dinitrosalicylic acid) methods, using maltose as standard solution. Two isolates (out of 13 amylolytic bacteria), BR 002 and BR 015 showed amylolytic index of 0.8 mm and 0.5 mm respectively, after being incubated at 55°C in the 0.002% Starch Agar Medium. The α-Amylase activity was further characterized quantitatively which includes the optimum condition of pH and temperature of α-Amylase crude enzyme from each isolate. To our knowledge, this is the first report on isolation and characterization of a thermostable α-Amylase from thermophilic bacteria isolated from Central Sulawesi particularly from Bora hot spring.

Targeted removal of ant colonies in ecological experiments, using hot water.

Science.gov (United States)

Tschinkel, Walter R; King, Joshua R

2007-01-01

Ecological experiments on fire ants cannot, or should not, use poison baits to eliminate the fire ants because such baits are not specific to fire ants, or even to ants. Hot water is an extremely effective and specific killing agent for fire ant colonies, but producing large amounts of hot water in the field, and making the production apparatus mobile have been problematical. The construction and use of a charcoal-fired kiln made from a 55-gal. oil drum lined with a sand-fireclay mixture is described. An automobile heater fan powered from a 12-v battery provided a draft. Dual bilge pumps pumped water from a large tank through a long coil of copper tubing within the kiln to produce 4 to 5 l. of hot water per min. The hot water was collected in 20 l. buckets and poured into fire ant nests previously opened by piercing with a stick. The entire assembly was transported in and operated from the back of a pickup truck. Five experimental plots containing 32 to 38 colonies of the fire ant, Solenopsis invicta, Buren (Hymenoptera: Formicidae), were treated with hot water over a period of two years. All colonies on the treatment plots were treated twice with hot water early in 2004, reducing their numbers to zero. However new colonies were formed, and mature colonies expanded into the plots. A third treatment was made in the spring of 2005, after which fire ant populations were suppressed for over a year. Whereas the 5 control plots contained a total of 166 mostly large colonies, the 5 treatment plots contained no live colonies at all. Averaged over a two-year period, a 70% reduction in total number of colonies was achieved (P ants.

Fiscal 1999 survey report on introducing technique for predicting impact on hot spring; 1999 nendo onsen eikyo yosoku shuho donyu chosa hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-10-01

For the application of fruits of the geothermal development promotion project to survey phase C and for the study of technical means for appropriate development to employ after phase C, some cases of impacts imposed on hot springs by geothermal development were taken up and the causes of the impacts were investigated. Activities were conducted in the three fields of (1) the survey of actualities of impacts imposed on hot springs, (2) the survey of the causes of such impacts, and (3) a comprehensive survey. Keyword searches were conducted into the data system and geothermal energy related magazines, and 13 cases were found in which hot springs were affected by geothermal development, which included the Palinpinon district (Philippines), the Koso district (America), and the Wairakei district (New Zealand). Concerning the 13 cases, data on geology, geological structures, and geothermal fluids were collected and studies were conducted about relations of geothermal development with geological structures and geothermal fluids, as in the case of hot springs, and the two were integrated for the clarification of the causes of impacts. In concluding the report, the difference in mechanism is deliberated between cases with impacts on hot springs and cases without impacts on hot springs. (NEDO)

Serratia sp. ZF03: an efficient radium biosorbent isolated from hot-spring waters in high background radiation areas.

Science.gov (United States)

Zakeri, Farideh; Noghabi, Kambiz Akbari; Sadeghizadeh, Majid; Kardan, Mohammad Reza; Masoomi, Fatemeh; Farshidpour, Mohammad Reza; Atarilar, Ali

2010-12-01

The aim of this study is to isolate and characterize (226)Ra biosorbing indigenous bacterial strains from soils and hot-springs containing high concentrations of (226)Ra by using biochemical and molecular approaches. Fifteen bacteria were isolated and their phylogenetic affiliations were determined based on their 16S rRNA gene and the two most relevant hypervariable regions of this gene; V3 and V6 analysis. A pigmented Serratia sp. ZF03 strain isolated from the water with (226)Ra content of 50471 mBq l(-1), caused 70% removal of (226)Ra at a radioactivity level of 50 Bq ml(-1), after 5 min and 75-80% in equilibrium time of 1 h, depending on the particular biosorption system and experimental conditions studied. The biosorption equilibrium was described by Langmuir and Freundlich isotherm models. Kinetic studies indicated that the biosorption follows pseudo-second-order kinetics. Effect of different physico-chemical parameters on (226)Ra sorption, FTIR, SEM and TEM analysis were also investigated. 2010 Elsevier Ltd. All rights reserved.

Preferential soft-tissue preservation in the Hot Creek carbonate spring deposit, British Columbia, Canada

Science.gov (United States)

Rainey, Dustin K.; Jones, Brian

2010-05-01

The relict Holocene Hot Creek carbonate spring deposit in southeast British Columbia is characterized by excellent preservation of soft-tissue organisms (e.g. cyanobacteria), but poor preservation of organisms with hard-tissue (e.g. wood, diatoms). The deposit is formed mainly of calcified cyanobacteria, with fewer mineralized macrophytes (plants), bryophytes (mosses), wood, and diatoms. Cyanobacteria grew as solitary filaments ( Lyngbya) and as radiating hemispherical colonies ( Rivularia). Both were preserved by encrustation and encapsulation while alive, and as casts after filament death and decay. Sheath impregnation was rare to absent. Filament encrustation, whereby calcite crystals nucleated on, and grew away from the sheath exterior, produced moulds that replicated external filament morphology, but hastened filament decay. Filament encapsulation, whereby calcite nucleated in the vicinity of, and grew towards the encapsulated filament, promoted sheath preservation even after trichome decay. Subsequent calcite precipitation inside the hollow sheath generated sheath casts. The inability of mineralizing spring water to penetrate durable cell walls meant that bryophytes, macrophytes, and most wood was preserved by encrustation. Some wood resisted complete decay for several thousand years, and its lignified cell walls allowed rare permineralizations. Diatoms were not preserved in the relict deposit because the frustules were dissolved by the basic spring water. Amorphous calcium carbonate produced by photosynthetic CO 2 removal may have acted as nucleation sites for physicochemically precipitated calcite. Thus, metabolic activities of floral organisms probably initiated biotic mineralization, but continuous inorganic calcite precipitation on and in flora ensured that soft tissues were preserved.

Fiscal 1999 survey for promotion of geothermal energy development. Survey report of environmental impact survey - Hot spring fluctuation (No. B-5, Musadake district); 1999 nendo chinetsu kaihatsu sokushin chosa. Kankyo eikyo chosa hokokusho (onsen hendo) (No.B-5 Musadake chiiki)

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-12-01

Research boreholes N11-MD-3 and -4 were drilled in Shibetsu-cho, Shibetsu-gun, Hokkaido, in the period July 1999 through December 1999, and thermal water was examined at the sites in July and November 1999. For the purpose of detecting the impact of the said work on the environment, local hot springs were examined for their status before, during, and after the drilling period. Measurements were conducted in the period August 5, 1998, through December 31, 2000. It was found that no fluctuation in water temperature or yield attributable to the drilling was detected. Hot springs were examined for nine items including water temperature and pH level at four spots which were Kaiyo-dai, River Tenyu, and Otoko-yu and Onna-yu of Kawakita Hot Spring, when 23-31 measurements were performed from each of the four. (NEDO)

Hot and cold CO{sub 2}-rich mineral waters in Chaves geothermal area (northern Portugal)

Energy Technology Data Exchange (ETDEWEB)

Aires-Barros, Luis; Marques, Jose Manuel; Graca, Rui Cores; Matias, Maria Jose [Universidade Tecnica de Lisboa, Lab. de Mineralogia e Petrologia (LAMPIST), Lisboa (Portugal); Weijden, Cornelis H. van der; Kreulen, Rob [Utrecht Univ., Dept. of Geochemistry, Utrecht (Netherlands); Eggenkamp, Hermanus Gerardus M. [Utrecht Univ., Dept. of Geochemistry, Utrecht (Netherlands); Reading Univ., Postgraduate Research Inst. for Sedimentology, Reading (United Kingdom)

1998-02-01

In order to update the geohydrologic characterisation of Chaves geothermal area, coupled isotopic and chemical studies have been carried out on hot and cold CO{sub 2}-rich mineral waters discharging, in northern Portugal, along one of the major regional NNE-trending faults (the so-called Verin-Chaves-Penacova Depression). Based upon their location, and chemical and isotopic composition, the analysed waters can be divided into two groups. The northern group belongs to the HCO{sub 3}/Na/CO{sub 2}-rich type, and consists of the hot spring waters of Chaves and the cold spring waters of Vilarelho da Raia. The {delta}D and {delta}{sup 18}O values show that these waters are of meteoric origin. The lack of an {sup 18}O shift indicates that there is no evidence of water/rock interaction at high temperatures. The southern group includes the cold spring waters of Campilho/Vidago and Sabroso/Pedras Salgadas. Their chemistry is similar to that of the northern group but their heavier {delta}D and {delta}{sup 18}O values could be attributed to different recharge altitudes. Mixing between deep mineralised waters and dilute superficial waters of meteoric origin might explain the higher {sup 3}H activity found in the Vidago and Pedras Salgadas mineral waters. Alternatively, they could be mainly related to shallow underground flowpaths. The {delta}{sup 13}C values support a deep-seated origin for the CO{sub 2}. The {delta}{sup 37}Cl is comparable in all the mineral waters of the study areas, indicating a common origin of Cl. The {sup 87}Sr/{sup 86}Sr ratios in waters seem to be dominated by the dissolution of plagioclases or granitic rocks. (Author)

Ground water monitoring strategies at the Weldon Spring Site, Weldon Spring, Missouri

International Nuclear Information System (INIS)

Meyer, K.A. Jr.

1988-01-01

This paper presents ground water monitoring strategies at the Weldon Spring Site in east-central Missouri. The Weldon Spring Site is a former ordnance works and uranium processing facility. In 1987, elevated levels of inorganic anions and nitroaromatics were detected in ground water beneath the site. Studies are currently underway to characterize the hydrogeologic regime and to define ground water contamination. The complex hydrogeology at the Weldon Spring Site requires innovative monitoring strategies. Combinations of fracture and conduit flow exist in the limestone bedrock. Perched zones are also present near surface impoundments. Losing streams and springs surround the site. Solving this complex combination of hydrogeologic conditions is especially challenging

Argillization by descending acid at Steamboat Springs, Nevada

Science.gov (United States)

Schoen, Robert; White, Donald E.; Hemley, J.J.

1974-01-01

Steamboat Springs, Nevada, an area of present-day hot springs, clearly illustrates the genetic dependence of some kaolin deposits on hot-spring activity. Andesite, granodiorite and arkosic sediments are locally altered at the land surface to siliceous residues consisting of primary quartz and anatase, plus opal from primary silicates. These siliceous residues commonly exhibit the textural and structural features of their unaltered equivalents. Beneath the siliceous residues, kaolin and alunite replace primary silicates and fill open spaces, forming a blanketlike deposit. Beneath the kaolin-alunite zone, montmorillonite, commonly accompanied by pyrite, replaces the primary silicates. On the ground surface, the same alteration mineral zones can be traced outward from the siliceous residue; however, hematite rather than pyrite accompanies montmorillonite.Chemical analysis indicates that sulfuric acid is the active altering agent. The acid forms from hydrogen sulfide that exsolves from deep thermal water, rises above the water table and is oxidized by sulfur-oxidizing bacteria living near the ground surface. This acid dissolves in precipitation or condensed water vapor and percolates downward destroying most of the primary minerals producing a siliceous residue. Coincidence of the water table with the downward transition from siliceous residue to kaolin-alunite signifies decreasing hydrogen metasomatism because of dilution of descending acid by ground water.In hot-spring areas, beds of siliceous sinter deposited at the surface by hypogene thermal water look, superficially, like areas of surficial acid alteration. Features diagnostic of a surficial alteration are the relict rock structures of a siliceous residue and a kaolin-alunite zone immediately beneath.

Water-chemistry and on-site sulfur-speciation data for selected springs in Yellowstone National Park, Wyoming, 1994-1995

Science.gov (United States)

Ball, James W.; Nordstrom, D. Kirk; Cunningham, Kirk M.; Schoonen, Martin A.; Xu, Yong; DeMonge, Jennifer M.

1998-01-01

Forty-two water analyses are reported for samples collected at 8 hot springs and their overflow drainages, two geysers, and two ambient-temperature acid streams in Yellowstone National Park during 1994-95. These water samples were collected and analyzed as part of the initial research investigations on sulfur redox speciation in the hot springs of Yellowstone and to document chemical changes in overflows that affect major ions, redox species, and trace elements. The sulfur redox speciation research is a collaboration between the State University of New York (SUNY) at Stony Brook and the U.S. Geological Survey (USGS). Four hot springs, Ojo Caliente, Azure, Frying Pan, and Angel Terrace, were studied in detail. Analyses were performed adjacent to the sampling site or in an on-site mobile lab truck constructed by the USGS, or later in a USGS laboratory. Water temperature, specific conductance, pH, Eh, D.O., and dissolved H2S were determined adjacent to the sample source at the time of sampling. Alkalinity and F- were determined on-site on the day of sample collection. Thiosulfate and polythionates were determined as soon as possible (minutes to hours later) by ion chromatography (IC). Other major anions (Cl-, SO4 2-, Br-) also were determined on-site by IC within two days of sample collection. Ammonium, Fe(II), and Fe(total) were determined on-site by ultraviolet/visible spectrophotometry within two days of sample collection. Later in the USGS laboratory, densities were determined. Concentrations of Ca, Mg, Li, Na, and K were determined by flame atomic absorption and emission (Na, K) spectrometry. Concentrations of Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe(total), K, Mg, Mn, Na, Ni, Pb, Si, Sr, V, and Zn were determined by inductively-coupled plasma optical emission spectrometry. Trace concentrations of Al and Mg were determined by Zeeman-corrected graphite furnace atomic absorption spectrometry. Three important conclusions from the sampling and analyses are: (1

Recovery Act Validation of Innovative Exploration Techniques Pilgrim Hot Springs, Alaska

Energy Technology Data Exchange (ETDEWEB)

Holdmann, Gwen [Univ. of Alaska, Fairbanks, AK (United States)

2015-04-30

Drilling and temperature logging campaigns between the late 1970's and early 1980’s measured temperatures at Pilgrim Hot Springs in excess of 90°C. Between 2010 and 2014 the University of Alaska used a variety of methods including geophysical surveys, remote sensing techniques, heat budget modeling, and additional drilling to better understand the resource and estimate the available geothermal energy.

Some geophysical and geological studies of the Tanzawa Mountains. [Nakagawa Hot Spring area, Hokizawa, and Higashizawa

Energy Technology Data Exchange (ETDEWEB)

Minakami, T; Matsuda, T; Hiraga, S; Horai, K I; Sugita, M

1964-11-01

Joints and zeolite-veins in both metamorphic rocks and quartz diorite exposed along the Nakagawa River were studied. Fractures with zeolite-veins are most developed in three areas, the Nakagawa hot spring area, Hokizawa, and Higashizawa. They follow two prevailing directions: N--S with minor right-lateral displacement and N60/sup 0/E with minor left-lateral displacement. The two fractures should represent a conjugate set that was produced by stress with maximum principal axis of N30/sup 0/E-S30/sup 0/W. Distribution and prevailing directions of fractures are illustrated. Geothermal gradients are measured in two newly opened boreholes, at the Nakagawa hot spring area and Higashizawa. The geothermal gradients are 12.60 +- 0.48/sup 0/C/100m at the Nakagawa hot spring and 5.55 +- 0.24/sup 0/C/100m at Higashizawa. Temperature-depth relationships in the two boreholes are given. Seismic observation was made at the Higashizawa. In five days 43 shocks were recorded, of which 20 are thought to have occurred 2 to 20km from the observation station, that is, in and very near the Tanzawa mountains. None have shallower hypocenters than 2 km in depth.

Calculation of the relative chemical stabilities of proteins as a function of temperature and redox chemistry in a hot spring.

Directory of Open Access Journals (Sweden)

Jeffrey M Dick

Full Text Available Uncovering the chemical and physical links between natural environments and microbial communities is becoming increasingly amenable owing to geochemical observations and metagenomic sequencing. At the hot spring known as Bison Pool in Yellowstone National Park, the cooling of the water in the outflow channel is associated with an increase in oxidation potential estimated from multiple field-based measurements. Representative groups of proteins whose sequences were derived from metagenomic data also exhibit an increase in average oxidation state of carbon in the protein molecules with distance from the hot-spring source. The energetic requirements of reactions to form selected proteins used in the model were computed using amino-acid group additivity for the standard molal thermodynamic properties of the proteins, and the relative chemical stabilities of the proteins were investigated by varying temperature, pH and oxidation state, expressed as activity of dissolved hydrogen. The relative stabilities of the proteins were found to track the locations of the sampling sites when the calculations included a function for hydrogen activity that increases with temperature and is higher, or more reducing, than values consistent with measurements of dissolved oxygen, sulfide and oxidation-reduction potential in the field. These findings imply that spatial patterns in the amino acid compositions of proteins can be linked, through energetics of overall chemical reactions representing the formation of the proteins, to the environmental conditions at this hot spring, even if microbial cells maintain considerably different internal conditions. Further applications of the thermodynamic calculations are possible for other natural microbial ecosystems.

Structural Controls of Neal Hot Springs Geothermal Field, Malhuer County, Oregon

Science.gov (United States)

Edwards, J. H.; Faulds, J. E.

2012-12-01

Detailed mapping (1:24,000) of the Neal Hot Springs area (90 km2) in eastern Oregon is part of a larger study of geothermal systems in the Basin and Range, which focuses on the structural controls of geothermal activity. The study area lies within the intersection of two regional grabens, the middle-late Miocene, N-striking, Oregon-Idaho graben and younger late Miocene to Holocene, NW-striking, western Snake River Plain graben. The geothermal field is marked by Neal Hot Springs, which effuse from opaline sinter mounds just north of Bully Creek. Wells producing geothermal fluids, with temperatures at 138°C, intersect a major, W-dipping, NNW-striking, high-angle normal fault at depths of 850-915 m. Displacement along this structure dies southward, with likely horse-tailing, which commonly produces high fracture density and a zone of high permeability conducive for channeling hydrothermal fluids. Mapping reveals that the geothermal resource lies within a local, left step-over. 'Hard-linkage' between strands of the left-stepping normal fault, revealed through a study of well chips and well logs, occurs through two concealed structures. Both are W-striking faults, with one that runs parallel to Cottonwood Creek and one 0.5 km N of the creek. Injection wells intersect these two transverse structures within the step-over. Stepping and displacement continue to the NW of the known geothermal field, along W-dipping, N-striking faults that cut lower to middle Miocene Hog Creek Formation, consisting of silicic and mafic volcanic rocks. These N-striking faults were likely initiated during initial Oregon-Idaho graben subsidence (15.3-15.1 Ma), with continued development through late Miocene. Bully Creek Formation deposits, middle to upper Miocene lacustrine and pyroclastic rocks, concomitantly filled the sub half-grabens, and they dip gently to moderately eastward. Younger, western Snake River Plain deposits, upper Miocene to Pliocene fluvial, lacustrine, and pyroclastic rocks

Microscopic Examination of Distribution and Phenotypic Properties of Phylogenetically Diverse Chloroflexaceae-Related Bacteria in Hot Spring Microbial Mats

DEFF Research Database (Denmark)

Nübel, U.; Bateson, Mary M.; Vandieken, V.

2002-01-01

We investigated the diversity, distribution, and phenotypes of uncultivated Chloroflexaceae-related bacteria in photosynthetic microbial mats of an alkaline hot spring (Mushroom Spring, Yellowstone National Park). By applying a directed PCR approach, molecular cloning, and sequence analysis of 16S...

Sanitary hot water; Eau chaude sanitaire

Energy Technology Data Exchange (ETDEWEB)

NONE

2004-07-01

Cegibat, the information-recommendation agency of Gaz de France for building engineering professionals, has organized this conference meeting on sanitary hot water to present the solutions proposed by Gaz de France to meet its clients requirements in terms of water quality, comfort, energy conservation and respect of the environment: quantitative aspects of the hot water needs, qualitative aspects, presentation of the Dolce Vita offer for residential buildings, gas water heaters and boilers, combined solar-thermal/natural gas solutions, key-specifications of hot water distribution systems, testimony: implementation of a gas hot water reservoir and two accumulation boilers in an apartment building for young workers. (J.S.)

Pediatric deep burns caused by hot incense ashes during 2014 Spring Festival in Fuyang city, China.

Science.gov (United States)

Wang, Jian; Zhou, Bo; Tao, Ren Qin; Chen, Xu Lin

2016-01-01

The Chinese people in Fuyang city, a northwest city of Anhui Province, are accustomed to burning incense at home for blessing during the Spring Festival. Their children, especially toddlers, like playing around the burning incense and are at risk of burning by hot incense ashes. The purpose of this study was to describe the unique cause and clinical characteristics of pediatric deep burns caused by hot incense ashes during 2014 Spring Festival. Twelve consecutive children admitted to our Burn Center and Fuyang People's Hospital during 2014 Spring Festival, with burn injuries caused by hot incense ashes which were epidemiologically studied retrospectively. Data on age, gender, size, depth and site of burn, incidence by day, number of operation, hospital stay, and causes of burns were collected. All patients came from Fuyang city. Of the 12 patients, the average age was 2.17 years, with a range of 1-6. The boy-to-girl ratio was 2: 1. The mean total burn surface area (TBSA) was 5.83%, and 91.67% of the children sustained full-thickness burn. Hands were the most common parts of the body to be injured. Dry necrosis developed in 14 fingers of 3 patients. January 31, 2014, the first day of the Chinese New Year, was the time of highest incidence. Six patients (50%) required surgical intervention while the number of operations including escharectomy, excision, skin grafting, or amputation of necrotic fingers, per patient was 2. A total of 14 fingers were amputated of the necrotic parts. All children survived and mean length of hospital stay of the patients was 20 days. Hot incense ashes cause serious injuries to children in Fuyang city during the Spring Festival. Preventive programs should be directed towards high risk groups to reduce the incidence of this burn.

Alaska Open-File Report 127 Assessment of Thermal Springs Sites in Southern Southeastern Alaska - Preliminary Results and Evaluation

Energy Technology Data Exchange (ETDEWEB)

Motyka, Roman J.; Moorman, Mary A.; Reeder, John W.

1980-06-01

Information has been gathered on 13 reported thermal-spring sites, 12 in southern Southeastern Alaska and one in western British Columbia. Five of the reported sites could not be substantiated by DGGS. The eight known thermal spring sites are associated with grainitic terrain and, except for Baker Island Hot Springs, occur within or near intensively fractured Cretaceous-age pluons of the Coast Range Batholith. Thermal-spring surface temperatures range from 21 C (Twin Lakes) to 91.5 C (Bailey Bay). The greatest discharge occurs at Chief Shakes hot springs (450 1pm). Bell Island Hot Springs, which has about a 100-1 pm discharge and a 70 C temperature, has had the most development. Two previously unreported thermal-spring sites, Barnes Lake warm springs and Bradfield hot springs, have a low rate of discharge and respective surface temperatures of about 25 and 54 C. The known thermal springs probably originate from circulation of meteoric waters through deep-seated fracture and fault systems. The chemical constituents of the alkali-sulfate to alkali-chloride thermal waters are probably derived from interaction of the deeply circulating meteoric waters with the granitic wall rocks. Chemical geothermometry suggests subsurface temperatures of 55 to 151 C. If waters are being heated solely by conduction from wall rocks, circulation depths must be about 1.5 to 5 km, assuming geothermal gradients of 30 to 50 C/km. Variations in temperature, discharge, and chemistry were noted at several thermal springs for which previous records are available. A major decrease in silica and potassium concentrations at Chief Shakes hot springs is suggested by comparing recent analyses of water chemistry to Waring's (1917) original analysis. The rate of discharge at Bell Island Hot Springs may have increased by a factor of two since Waring's visit to the springs. Subsurface reservoirs associated with thermal springs in southern Southeastern Alaska are of low temperature and are

A comprehensive census of microbial diversity in hot springs of Tengchong, Yunnan Province China using 16S rRNA gene pyrosequencing.

Science.gov (United States)

Hou, Weiguo; Wang, Shang; Dong, Hailiang; Jiang, Hongchen; Briggs, Brandon R; Peacock, Joseph P; Huang, Qiuyuan; Huang, Liuqin; Wu, Geng; Zhi, Xiaoyang; Li, Wenjun; Dodsworth, Jeremy A; Hedlund, Brian P; Zhang, Chuanlun; Hartnett, Hilairy E; Dijkstra, Paul; Hungate, Bruce A

2013-01-01

The Rehai and Ruidian geothermal fields, located in Tengchong County, Yunnan Province, China, host a variety of geochemically distinct hot springs. In this study, we report a comprehensive, cultivation-independent census of microbial communities in 37 samples collected from these geothermal fields, encompassing sites ranging in temperature from 55.1 to 93.6°C, in pH from 2.5 to 9.4, and in mineralogy from silicates in Rehai to carbonates in Ruidian. Richness was low in all samples, with 21-123 species-level OTUs detected. The bacterial phylum Aquificae or archaeal phylum Crenarchaeota were dominant in Rehai samples, yet the dominant taxa within those phyla depended on temperature, pH, and geochemistry. Rehai springs with low pH (2.5-2.6), high temperature (85.1-89.1°C), and high sulfur contents favored the crenarchaeal order Sulfolobales, whereas those with low pH (2.6-4.8) and cooler temperature (55.1-64.5°C) favored the Aquificae genus Hydrogenobaculum. Rehai springs with neutral-alkaline pH (7.2-9.4) and high temperature (>80°C) with high concentrations of silica and salt ions (Na, K, and Cl) favored the Aquificae genus Hydrogenobacter and crenarchaeal orders Desulfurococcales and Thermoproteales. Desulfurococcales and Thermoproteales became predominant in springs with pH much higher than the optimum and even the maximum pH known for these orders. Ruidian water samples harbored a single Aquificae genus Hydrogenobacter, whereas microbial communities in Ruidian sediment samples were more diverse at the phylum level and distinctly different from those in Rehai and Ruidian water samples, with a higher abundance of uncultivated lineages, close relatives of the ammonia-oxidizing archaeon "Candidatus Nitrosocaldus yellowstonii", and candidate division O1aA90 and OP1. These differences between Ruidian sediments and Rehai samples were likely caused by temperature, pH, and sediment mineralogy. The results of this study significantly expand the current understanding of

The Phosphoria Formation at the Hot Springs Mine in Southeast Idaho; a source of selenium and other trace elements to surface water, ground water, vegetation, and biota

Science.gov (United States)

Piper, David Z.; Skorupa, J.P.; Presser, T.S.; Hardy, M.A.; Hamilton, S.J.; Huebner, M.; Gulbrandsen, R.A.

2000-01-01

Major-element oxides and trace elements in the Phosphoria Formation at the Hot Springs Mine, Idaho were determined by a series of techniques. In this report, we examine the distribution of trace elements between the different solid components aluminosilicates, apatite, organic matter, opal, calcite, and dolomite that largely make up the rocks. High concentrations of several trace elements throughout the deposit, for example, As, Cd, Se, Tl, and U, at this and previously examined sites have raised concern about their introduction into the environment via weathering and the degree to which mining and the disposal of mined waste rock from this deposit might be accelerating that process. The question addressed here is how might the partitioning of trace elements between these solid host components influence the introduction of trace elements into ground water, surface water, and eventually biota, via weathering? In the case of Se, it is partitioned into components that are quite labile under the oxidizing conditions of subaerial weathering. As a result, it is widely distributed throughout the environment. Its concentration exceeds the level of concern for protection of wildlife at virtually every trophic level.

Factors controlling the distribution of archaeal tetraethers in terrestrial hot springs.

Science.gov (United States)

Pearson, Ann; Pi, Yundan; Zhao, Weidong; Li, WenJun; Li, Yiliang; Inskeep, William; Perevalova, Anna; Romanek, Christopher; Li, Shuguang; Zhang, Chuanlun L

2008-06-01

Glycerol dialkyl glycerol tetraethers (GDGTs) found in hot springs reflect the abundance and community structure of Archaea in these extreme environments. The relationships between GDGTs, archaeal communities, and physical or geochemical variables are underexamined to date and when reported often result in conflicting interpretations. Here, we examined profiles of GDGTs from pure cultures of Crenarchaeota and from terrestrial geothermal springs representing a wide distribution of locations, including Yellowstone National Park (United States), the Great Basin of Nevada and California (United States), Kamchatka (Russia), Tengchong thermal field (China), and Thailand. These samples had temperatures of 36.5 to 87 degrees C and pH values of 3.0 to 9.2. GDGT abundances also were determined for three soil samples adjacent to some of the hot springs. Principal component analysis identified four factors that accounted for most of the variance among nine individual GDGTs, temperature, and pH. Significant correlations were observed between pH and the GDGTs crenarchaeol and GDGT-4 (four cyclopentane rings, m/z 1,294); pH correlated positively with crenarchaeol and inversely with GDGT-4. Weaker correlations were observed between temperature and the four factors. Three of the four GDGTs used in the marine TEX(86) paleotemperature index (GDGT-1 to -3, but not crenarchaeol isomer) were associated with a single factor. No correlation was observed for GDGT-0 (acyclic caldarchaeol): it is effectively its own variable. The biosynthetic mechanisms and exact archaeal community structures leading to these relationships remain unknown. However, the data in general show promise for the continued development of GDGT lipid-based physiochemical proxies for archaeal evolution and for paleo-ecology or paleoclimate studies.

Hydrogeological controls of radon in a few hot springs in the Western Ghats at Ratnagiri district in Maharashtra, India

International Nuclear Information System (INIS)

Ansari, Md. Arzoo; Sharma, Suman; Saravana Kumar, U.; Chatterjee, Sitangshu; Diksha; Low, Upananda

2014-01-01

Geological structures (faults, fractures and weak zones) and high heat flow in geothermal areas allow easy passage for release of radon gas to the atmosphere. Radon is constantly transported from the Earth's interior and vented out through exhalation points at permeable fault zones. 222 Rn concentrations were measured in a few hot springs and nearby groundwater using RAD7 at Tural and Rajwadi, Ratnagiri district, Maharashtra. The 222 Rn concentrations in the hot springs vary from 1087 ± 132 to 1655 ± 177 Bq/m 3 at Tural and from 152 ± 67 to 350 ± 82 Bq/m 3 at Rajwadi. Groundwaters from wells within a radius of 200 m around the geothermal fields have radon concentration between 1087 ± 132 and 5445 ± 337 Bq/m 3 . We have assessed the radon activity in the vicinity of the hot springs to understand their hydrogeological control, origin of heat source and possible effect on the tourist and the human population residing nearby. (author)

Direct uses of hot water (geothermal) in dairying

Energy Technology Data Exchange (ETDEWEB)

Barmettler, E.R.; Rose, W.R. Jr.

1978-01-01

Digital computer simulation was used to investigate the peak, steady energy utilization of a geothermal energy-supported dairy. A digital computer program was also written to assess the lifetime economics of the dairy operation. A dynamic simulation program was written to design water storage tanks under diurnal transient loading. The geothermal site specified is the artesian spring named Hobo Wells near Susanville, California. The dairy configuration studies are unique, but consist of conventional processing equipment. In the dairy, cattle waste would be used to generate methane and carbon dioxide by anaerobic digestion. Some carbon dioxide would be removed from the gas stream with a pressurized water scrubber to raise the heating value. The product gas would be combusted in a spark ignition engine connected to an electric generator. The electrical power produced would be used for operation of fans, pumps, lights and other equipment in the dairy. An absorption chiller using a geothermal water driven generator would provide milk chilling. Space heating would be done with forced air hot water unit heaters.

Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils

OpenAIRE

Aanniz,Tarik; Ouadghiri,Mouna; Melloul,Marouane; Swings,Jean; Elfahime,Elmostafa; Ibijbijen,Jamal; Ismaili,Mohamed; Amar,Mohamed

2015-01-01

The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. ...

OUT Success Stories: Solar Hot Water Technology

International Nuclear Information System (INIS)

Clyne, R.

2000-01-01

Solar hot water technology was made great strides in the past two decades. Every home, commercial building, and industrial facility requires hot water. DOE has helped to develop reliable and durable solar hot water systems. For industrial applications, the growth potential lies in large-scale systems, using flat-plate and trough-type collectors. Flat-plate collectors are commonly used in residential hot water systems and can be integrated into the architectural design of the building

OUT Success Stories: Solar Hot Water Technology

Science.gov (United States)

Clyne, R.

2000-08-01

Solar hot water technology was made great strides in the past two decades. Every home, commercial building, and industrial facility requires hot water. DOE has helped to develop reliable and durable solar hot water systems. For industrial applications, the growth potential lies in large-scale systems, using flat-plate and trough-type collectors. Flat-plate collectors are commonly used in residential hot water systems and can be integrated into the architectural design of the building.

Typing of the sausage-shaped bacteria forming A-type sulfur-turf according to cell length distributions of natural populations and physico-chemical conditions of hot spring waters; Saibo chobunpu to seiiku kankyo kara mita A gata io shiba shizen kotaigun ni okeru okamagata saikin no katabetsu ni tsuite

Energy Technology Data Exchange (ETDEWEB)

Aki, Y. [Iwate University, Iwate (Japan). Faculty of Humanities and Social Sciences

1996-01-25

In order to type the sausage-shaped bacteria forming A-type sulfur-turf, cell length distributions and physics-chemical conditions of hot spring waters were investigated on twelve samples collected from all over Japan. The frequency distributions of the cell length of eight samples were bimodal, while the other four samples were unimodal. In seven samples with bimodal distributions, two types (large and small) of the sausage-shaped bacteria could be differentiated. The cell length of the large-type was between 10.1 and 31.9{mu}m, while that of the small-type ranged 2.2 to 6.6{mu}m. The pH of seven hot spring waters were between 6 and 8, and the two types (large and small) formed together sulfur-turf. In contrast, pH of the three hot springs were over 8, and the two types of the sausage-shaped bacteria could not be detected in the sulfur-turf. Therefore, it is reasonable to set a third type of the sausage-shaped bacteria which prefers high-pH (over 8) and low calcium condition. The cell lengths of the third type were in the range of 5.5 to 8.6{mu}m, which correspond to the sausage-shaped bacteria of medium size. 20 refs., 5 figs., 2 tabs.

Ammonia oxidation driven by archaea rather than bacteria in the hot spring at Tengchong geothermal field, China.

Science.gov (United States)

Chen, Shun; Peng, Xiaotong; Xu, Hengchao; Li, Jiwei; Ta, Kaiwen

2015-04-01

The occurrence of microbial mediated ammonia oxidation and these organisms are present in large numbers in natural environments indicated a potential biogeochemical role for them in the global nitrogen cycle. However, very little is understood about their role and contribution to nitrification in the high temperature extreme environments. Here we explore the ammonia oxidation rates and abundance of potential ammonia-oxidizing archaea (AOA) in upper and bottom sediments from Gongxiaoshe hot spring, Tengchong, Yunnan, China. The 15N-incorporating AOA cells and cell aggregated were detected with Fluorescence in situ hybridization (FISH) and Nano secondary ion mass spectrometry (Nano-SIMS). Ammonia oxidation rates measured using 15N-NO3- pool dilution in upper and bottom sediments (without NH4+ stimulated) were 4.8 and 5.3 nmol N g-1h-1, respectively. Close relatives of the autotrophic, ammonia-oxidizing archaeon 'Candidatus Nitrosocaldus yellowstonii' represented the most abundant OTU in both of the two spring sediments by 16S rRNA gene analysis. Furthermore, it should be noted that no ammonia-oxidizing bacterial clones detected in this study. Quantitative PCR (qPCR) indicated that AOA and 16S rRNA genes were present at 2.75-9.80×105 and 0.128-1.96×108 gene copies g-1 sediment. Based on the reaction rates and AOA abundance, we estimated the cell-specific nitrification rates were 0.41 to 0.79 fmol N archaeal cell-1 h-1, which are comparable to those observed in estuary environment. We suggest that AOA have the responsibility in nitrification in this hot spring, and these archaea rather than bacteria may be considered as a driver in nitrogen cycling in terrestrial hot ecosystems. Key words: ammonia-oxidizing archaea (AOA); nitrification; ammonia-oxidizing rate; hot spring;

Arsenic Removal from Drinking Water by Adsorptive Media - U.S. EPA Demonstration Project at Hot Springs Mobile Home Park in Willard, Utah - Final Performance Evaluation Report

Science.gov (United States)

This report documents activities performed for and results obtained from the arsenic removal treatment technology demonstration project at the Hot Springs Mobile Home Park (HSMHP) in Willard, UT. The objectives of the project were to evaluate the effectiveness of Adsorbsia™ GTO™...

Molecular diversity of thermophilic bacteria isolated from Pasinler hot spring (Erzurum, Turkey)

OpenAIRE

ADIGÜZEL, Ahmet; İNAN, Kadriye; ŞAHİN, Fikrettin; ARASOĞLU, Tulin; GÜLLÜCE, Medine

2011-01-01

The present study was conducted to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Pasinler hot spring, Erzurum, Turkey. Fatty acid profiles, BOX PCR fingerprints, and 16S rDNA sequence data were used for the phenotypic and genotypic characterization of thermophilic bacteria. Totally 9 different bacterial strains were selected based on morphological, physiological, and biochemical tests. These strains were characterized by molecular tests includi...

Stable isotope labeling confirms mixotrophic nature of streamer biofilm communities at alkaline hot springs

Directory of Open Access Journals (Sweden)

Florence eSchubotz

2015-02-01

Full Text Available Streamer biofilm communities (SBC are often observed within chemosynthetic zones of Yellowstone hot spring outflow channels, where temperatures exceed those conducive to photosynthesis. Nearest the hydrothermal source (75-88°C SBC comprise thermophilic Archaea and Bacteria, often mixed communities including Desulfurococcales and uncultured Crenarchaeota, as well as Aquificae, Thermus, each carrying diagnostic membrane lipid biomarkers. We tested the hypothesis that SBC can alternate their metabolism between autotrophy and heterotrophy depending on substrate availability. Feeding experiments were performed at two alkaline hot springs in Yellowstone National Park: Octopus Spring and ‘Bison Pool’, using various 13C-labeled substrates (bicarbonate, formate, acetate and glucose to determine the relative uptake of these different carbon sources. Highest 13C uptake, at both sites, was from acetate into almost all bacterial fatty acids, particularly into methyl-branched C15, C17 and C19 fatty acids that are diagnostic for Thermus/Meiothermus and some Firmicutes as well as into universally common C16:0 and C18:0 fatty acids. 13C-glucose showed a similar, but a 10 to 30 times lower uptake across most fatty acids. 13C bicarbonate uptake, signifying the presence of autotrophic communities was only significant at ‘Bison Pool’ and was observed predominantly in non-specific saturated C16, C18, C20 and C22 fatty acids. Incorporation of 13C-formate occurred only at very low rates at ‘Bison Pool’ and was almost undetectable at Octopus Spring, suggesting that formate is not an important carbon source for SBC. 13C uptake into archaeal lipids occurred predominantly with 13C acetate, suggesting also that archaeal communities at both springs have primarily heterotrophic carbon assimilation pathways. We hypothesize that these communities are energy-limited and predominantly nurtured by input of exogenous organic material, with only a small fraction being

Streptomyces caldifontis sp. nov., isolated from a hot water spring of Tatta Pani, Kotli, Pakistan.

Science.gov (United States)

Amin, Arshia; Ahmed, Iftikhar; Khalid, Nauman; Osman, Ghenijan; Khan, Inam Ullah; Xiao, Min; Li, Wen-Jun

2017-01-01

A Gram-staining positive, non-motile, rod-shaped, catalase positive and oxidase negative bacterium, designated NCCP-1331 T , was isolated from a hot water spring soil collected from Tatta Pani, Kotli, Azad Jammu and Kashmir, Pakistan. The isolate grew at a temperature range of 18-40 °C (optimum 30 °C), pH 6.0-9.0 (optimum 7.0) and with 0-6 % NaCl (optimum 2 % NaCl (w/v)). The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain NCCP-1331 T belonged to the genus Streptomyces and is closely related to Streptomyces brevispora BK160 T with 97.9 % nucleotide similarity, followed by Streptomyces drosdowiczii NRRL B-24297 T with 97.8 % nucleotide similarity. The DNA-DNA relatedness values of strain NCCP-1331 T with S. brevispora KACC 21093 T and S. drosdowiczii CBMAI 0498 T were 42.7 and 34.7 %, respectively. LL-DAP was detected as diagnostic amino acid along with alanine, glycine, leucine and glutamic acid. The isolate contained MK-9(H 8 ) as the predominant menaquinone. Major polar lipids detected in NCCP-1331 T were phosphatidylethanolamine, phosphatidylinositol and unidentified phospholipids. Major fatty acids were iso-C 16: 0 , summed feature 8 (18:1 ω7c/18:1 ω6c), anteiso-C 15:0 and C 16:0 . The genomic DNA G + C content was 69.8 mol %. On the basis of phylogenetic, phenotypic and chemotaxonomic analysis, it is concluded that strain NCCP-1331 T represents a novel species of the genus Streptomyces, for which the name Streptomyces caldifontis sp. nov. is proposed. The type strain is NCCP-1331 T (=KCTC 39537 T  = CPCC 204147 T ).

21 CFR 880.6085 - Hot/cold water bottle.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Hot/cold water bottle. 880.6085 Section 880.6085... Devices § 880.6085 Hot/cold water bottle. (a) Identification. A hot/cold water bottle is a device intended for medical purposes that is in the form of a container intended to be filled with hot or cold water...

Solar Hot Water Heater

Science.gov (United States)

1978-01-01

The solar panels pictured below, mounted on a Moscow, Idaho home, are part of a domestic hot water heating system capable of providing up to 100 percent of home or small business hot water needs. Produced by Lennox Industries Inc., Marshalltown, Iowa, the panels are commercial versions of a collector co-developed by NASA. In an effort to conserve energy, NASA has installed solar collectors at a number of its own facilities and is conducting research to develop the most efficient systems. Lewis Research Center teamed with Honeywell Inc., Minneapolis, Minnesota to develop the flat plate collector shown. Key to the collector's efficiency is black chrome coating on the plate developed for use on spacecraft solar cells, the coating prevents sun heat from "reradiating," or escaping outward. The design proved the most effective heat absorber among 23 different types of collectors evaluated in a Lewis test program. The Lennox solar domestic hot water heating system has three main components: the array of collectors, a "solar module" (blue unit pictured) and a conventional water heater. A fluid-ethylene glycol and water-is circulated through the collectors to absorb solar heat. The fluid is then piped to a double-walled jacket around a water tank within the solar module.

A comprehensive census of microbial diversity in hot springs of Tengchong, Yunnan Province China using 16S rRNA gene pyrosequencing.

Directory of Open Access Journals (Sweden)

Weiguo Hou

Full Text Available The Rehai and Ruidian geothermal fields, located in Tengchong County, Yunnan Province, China, host a variety of geochemically distinct hot springs. In this study, we report a comprehensive, cultivation-independent census of microbial communities in 37 samples collected from these geothermal fields, encompassing sites ranging in temperature from 55.1 to 93.6°C, in pH from 2.5 to 9.4, and in mineralogy from silicates in Rehai to carbonates in Ruidian. Richness was low in all samples, with 21-123 species-level OTUs detected. The bacterial phylum Aquificae or archaeal phylum Crenarchaeota were dominant in Rehai samples, yet the dominant taxa within those phyla depended on temperature, pH, and geochemistry. Rehai springs with low pH (2.5-2.6, high temperature (85.1-89.1°C, and high sulfur contents favored the crenarchaeal order Sulfolobales, whereas those with low pH (2.6-4.8 and cooler temperature (55.1-64.5°C favored the Aquificae genus Hydrogenobaculum. Rehai springs with neutral-alkaline pH (7.2-9.4 and high temperature (>80°C with high concentrations of silica and salt ions (Na, K, and Cl favored the Aquificae genus Hydrogenobacter and crenarchaeal orders Desulfurococcales and Thermoproteales. Desulfurococcales and Thermoproteales became predominant in springs with pH much higher than the optimum and even the maximum pH known for these orders. Ruidian water samples harbored a single Aquificae genus Hydrogenobacter, whereas microbial communities in Ruidian sediment samples were more diverse at the phylum level and distinctly different from those in Rehai and Ruidian water samples, with a higher abundance of uncultivated lineages, close relatives of the ammonia-oxidizing archaeon "Candidatus Nitrosocaldus yellowstonii", and candidate division O1aA90 and OP1. These differences between Ruidian sediments and Rehai samples were likely caused by temperature, pH, and sediment mineralogy. The results of this study significantly expand the current

Hot water reticulation

Energy Technology Data Exchange (ETDEWEB)

Fellows, S. K.

1977-10-15

Hot water reticulation (district heating) is an established method of energy supply within cities in many countries. It is based on the fact that heat can often be obtained cheaply in bulk, and that the resultant savings can, in suitable circumstances, justify the investment in a reticulation network of insulated pipes to distribute the heat to many consumers in the form of hot water or occasionally steam. The heat can be used by domestic, commercial, and industrial consumers for space heating and water heating, and by industries for process heat. The costs of supplying domestic consumers can be determined by considering an average residential area, but industrial and commercial consumers are so varied in their requirements that every proposal must be treated independently. Fixed costs, variable costs, total costs, and demand and resource constraints are discussed.

The plumbing system of the Pagosa thermal Springs, Colorado: Application of geologically constrained geophysical inversion and data fusion

Science.gov (United States)

Revil, A.; Cuttler, S.; Karaoulis, M.; Zhou, J.; Raynolds, B.; Batzle, M.

2015-06-01

Fault and fracture networks usually provide the plumbing for movement of hydrothermal fluids in geothermal fields. The Big Springs of Pagosa Springs in Colorado is known as the deepest geothermal hot springs in the world. However, little is known about the plumbing system of this hot spring, especially regarding the position of the reservoir (if any) or the position of the major tectonic faults controlling the flow of the thermal water in this area. The Mancos shale, a Cretaceous shale, dominates many of the surface expressions around the springs and impede an easy recognition of the fault network. We use three geophysical methods (DC resistivity, self-potential, and seismic) to image the faults in this area, most of which are not recognized in the geologic fault map of the region. Results from these surveys indicate that the hot Springs (the Big Spring and a warm spring located 1.8 km further south) are located at the intersection of the Victoire Fault, a major normal crustal fault, and two north-northeast trending faults (Fault A and B). Self-potential and DC resistivity tomographies can be combined and a set of joint attributes defined to determine the localization of the flow of hot water associated with the Eight Miles Mesa Fault, a second major tectonic feature responsible for the occurrence of warm springs further West and South from the Big Springs of Pagosa Springs.

Draft genome sequence of Lampropedia cohaerens strain CT6(T) isolated from arsenic rich microbial mats of a Himalayan hot water spring.

Science.gov (United States)

Tripathi, Charu; Mahato, Nitish K; Rani, Pooja; Singh, Yogendra; Kamra, Komal; Lal, Rup

2016-01-01

Lampropedia cohaerens strain CT6(T), a non-motile, aerobic and coccoid strain was isolated from arsenic rich microbial mats (temperature ~45 °C) of a hot water spring located atop the Himalayan ranges at Manikaran, India. The present study reports the first genome sequence of type strain CT6(T) of genus Lampropedia cohaerens. Sequencing data was generated using the Illumina HiSeq 2000 platform and assembled with ABySS v 1.3.5. The 3,158,922 bp genome was assembled into 41 contigs with a mean GC content of 63.5 % and 2823 coding sequences. Strain CT6(T) was found to harbour genes involved in both the Entner-Duodoroff pathway and non-phosphorylated ED pathway. Strain CT6(T) also contained genes responsible for imparting resistance to arsenic, copper, cobalt, zinc, cadmium and magnesium, providing survival advantages at a thermal location. Additionally, the presence of genes associated with biofilm formation, pyrroloquinoline-quinone production, isoquinoline degradation and mineral phosphate solubilisation in the genome demonstrate the diverse genetic potential for survival at stressed niches.

Getting into hot water Problematizing hot water service demand: The case of Old Cairo

Science.gov (United States)

Culhane, Thomas Henry

This dissertation analyzes hot water demand and service infrastructure in two neighboring but culturally distinct communities of the urban poor in the inner-city area of central Cairo. The communities are the Historic Islamic Cairo neighborhood of Darb Al Ahmar at the foot of Al-Azhar park, and the Zurayib neighborhood of Manshiyat Nasser where the Coptic Zabaleen Recyclers live. The study focuses on the demand side of the hot water issue and involves consideration of built-environment infrastructures providing piped water, electricity, bottled gas, sewage, and the support structures (wiring and plumbing) for consumer durables (appliances such as hot water heaters, stoves, refrigerators, air conditioners) as well as water pumps and water storage tanks. The study asks the questions "How do poor communities in Cairo value hot water" and "How do cost, infrastructure and cultural preferences affect which attributes of hot water service are most highly preferred?". To answer these questions household surveys based primarily on the World Bank LSMS modules were administered by professional survey teams from Darb Al Ahmar's Aga Khan Trust for Culture and the Zabaleen's local NGO "Spirit of Youth" in their adjacent conununities in and surrounding historic Cairo. In total 463 valid surveys were collected, (231 from Darb Al Ahmar, 232 from the Zabaleen). The surveys included a contingent valuation question to explore Willingness to Pay for improved hot water service; the surveys queried household assets as proxies for income. The dissertation's findings reveal that one quarter of the residents of Darb Al Ahmar and two-thirds of the residents of Manshiyet Nasser's Zabaleen lack conventional water heating service. Instead they employ various types of stoves and self-built contraptions to heat water, usually incurring considerable risk and opportunity costs. However the thesis explores the notion that this is rational "satisficing" behavior; despite the shortcomings of such self

Thermal Water of Utah Topical Report

Energy Technology Data Exchange (ETDEWEB)

Goode, Harry D.

1978-11-01

Western and central Utah has 16 areas whose wells or springs yield hot water (35 C or higher), warm water (20-34.5 C), and slightly warm water (15.5-19.5 C). These areas and the highest recorded water temperature for each are: Lower Bear River Area, 105 C; Bonneville Salt Flats, 88 C; Cove Fort-Sulphurdale, 77 C; Curlew Valley, 43 C; East Shore Area, 60 C; Escalante Desert, 149 C; Escalante Valley (Roosevelt, 269 C, and Thermo, 85C); Fish Springs, 60.5 C; Grouse Creek Valley, 42 C; Heber Valley (Midway, 45 C); Jordan Valley, 58.5 C; Pavant Valley-Black Rock Desert, 67 C; Sevier Desert ( Abraham-Crater Hot Springs, 82 C); Sevier Valley (Monroe-Red Hill, 76.5 C, and Joseph Hot Spring, 64 C); Utah Valley, 46 C; and Central Virgin River Basin, 42 C. The only hot water in eastern Utah comes from the oil wells of the Ashley Valley Oil Field, which in 1977 yielded 4400 acre-feet of water at 43 C to 55 C. Many other areas yield warm water (20 to 34.5 C) and slightly warm water (15.5 to 19.5 C). With the possible exception of the Roosevelt KGRA, Crater Hot Springs in the Sevier Desert, Escalante Desert, Pavant-Black Rock, Cove Fort-Sulphurdale, and Coyote Spring in Curlew Valley, which may derive their heat from buried igneous bodies, the heat that warms the thermal water is derived from the geothermal gradient. Meteoric water circulates through fractures or permeable rocks deep within the earth, where it is warmed; it then rises by convection or artesian pressure and issues at the surface as springs or is tapped by wells. Most thermal springs thus rise along faults, but some thermal water is trapped in confined aquifers so that it spreads laterally as it mixes with and warms cooler near-surface water. This spreading of thermal waters is evident in Cache Valley, in Jordan Valley, and in southern Utah Valley; likely the spreading occurs in many other artesian basins where it has not yet been recognized. In the East Shore Area thermal water trapped in confined aquifers warms

Radiocarbon Ages and Environments of Deposition of the Wono and Trego Hot Springs Tephra Layers in the Pyramid Lake Subbasin, Nevada

Science.gov (United States)

Benson, L.V.; Smoot, J.P.; Kashgarian, Michaele; Sarna-Wojcicki, A.; Burdett, J.W.

1997-01-01

Uncalibrated radiocarbon data from core PLC92B taken from Wizards Cove in the Pyramid Lake subbasin indicate that the Trego Hot Springs and Wono tephra layers were deposited 23,200 ?? 300 and 27,300 ??300 14C yr B.P. (uncorrected for reservoir effect). Sedimentological data from sites in the Pyramid Lake and Smoke Creek-Black Rock Desert subbasins indicate that the Trego Hot Springs tephra layer was deposited during a relatively dry period when Pyramid Lake was at or below its spill point (1177 m) to the Winnemucca Lake subbasin. The Wono tephra layer was deposited when lake depth was controlled by spill across Emerson Pass sill (1207 m) to the Smoke Creek-Black Rock Desert subbasin. 18O data from core PLC92B also support the concept that the Trego Hot Springs tephra fell into a relatively shallow Pyramid Lake and that the Wono tephra fell into a deeper spilling lake. ?? 1997 University of Washington.

water quality evaluation of spring waters in nsukka, nigeria

African Journals Online (AJOL)

ES Obe

2013-07-02

Jul 2, 2013 ... directly from an underground formation from, which water flows naturally to the surface or from a bored ... evidence that surface runoff is readily entering the spring. This may mean the spring is contaminated with ... soil, hydrological factors that lead to runoff, and by biological processes within the aquatic.

Neutral sodium/bicarbonate/sulfate hot waters in geothermal systems

Energy Technology Data Exchange (ETDEWEB)

Mahon, W.A.J. (Dept. of Industrial and Scientific Research, Wairakei, New Zealand); Klyen, L.E.; Rhode, M.

1980-03-01

The least understood thermal water is a near neutral water which contains varying amounts of bicarbonate and sulfate as the major anions, low concentrations of chloride (< 30 ppM) and sodium as the major cation. In the past this water has been referred to as a sodium bicarbonate water but present studies suggest that the quantities of bicarbonate and sulfate in this water type are frequently of the same order. Of particular interest is the distribution and position of the sodium/bicarbonate/sulfate water in the same and different systems. Many hot springs in Indonesia, for example, discharge water of this composition. Present studies indicate that this water type can originate from high temperature reservoirs which form the secondary steam heated part of a normal high temperature geothermal system. The hydrological conditions producing these waters in geothermal systems are investigated and the relationship between the water type and vapor dominated systems is discussed. It is suggested that the major water type occurring in the so called vapor dominated parts of geothermal systems is this water. The water does not simply represent steam condensate, rather it consists essentially of meteoric water which has been steam heated. The water composition results from the interaction of carbon dioxide and hydrogen sulfide with meteoric water and the rocks confining this water in the aquifer.

Global Occurrence of Archaeal amoA Genes in Terrestrial Hot Springs▿

Science.gov (United States)

Zhang, Chuanlun L.; Ye, Qi; Huang, Zhiyong; Li, WenJun; Chen, Jinquan; Song, Zhaoqi; Zhao, Weidong; Bagwell, Christopher; Inskeep, William P.; Ross, Christian; Gao, Lei; Wiegel, Juergen; Romanek, Christopher S.; Shock, Everett L.; Hedlund, Brian P.

2008-01-01

Despite the ubiquity of ammonium in geothermal environments and the thermodynamic favorability of aerobic ammonia oxidation, thermophilic ammonia-oxidizing microorganisms belonging to the crenarchaeota kingdom have only recently been described. In this study, we analyzed microbial mats and surface sediments from 21 hot spring samples (pH 3.4 to 9.0; temperature, 41 to 86°C) from the United States, China, and Russia and obtained 846 putative archaeal ammonia monooxygenase large-subunit (amoA) gene and transcript sequences, representing a total of 41 amoA operational taxonomic units (OTUs) at 2% identity. The amoA gene sequences were highly diverse, yet they clustered within two major clades of archaeal amoA sequences known from water columns, sediments, and soils: clusters A and B. Eighty-four percent (711/846) of the sequences belonged to cluster A, which is typically found in water columns and sediments, whereas 16% (135/846) belonged to cluster B, which is typically found in soils and sediments. Although a few amoA OTUs were present in several geothermal regions, most were specific to a single region. In addition, cluster A amoA genes formed geographic groups, while cluster B sequences did not group geographically. With the exception of only one hot spring, principal-component analysis and UPGMA (unweighted-pair group method using average linkages) based on the UniFrac metric derived from cluster A grouped the springs by location, regardless of temperature or bulk water pH, suggesting that geography may play a role in structuring communities of putative ammonia-oxidizing archaea (AOA). The amoA genes were distinct from those of low-temperature environments; in particular, pair-wise comparisons between hot spring amoA genes and those from sympatric soils showed less than 85% sequence identity, underscoring the distinctness of hot spring archaeal communities from those of the surrounding soil system. Reverse transcription-PCR showed that amoA genes were

Residential hot water distribution systems: Roundtablesession

Energy Technology Data Exchange (ETDEWEB)

Lutz, James D.; Klein, Gary; Springer, David; Howard, Bion D.

2002-08-01

Residential building practice currently ignores the lossesof energy and water caused by the poor design of hot water systems. Theselosses include: combustion and standby losses from water heaters, thewaste of water (and energy) while waiting for hot water to get to thepoint of use; the wasted heat as water cools down in the distributionsystem after a draw; heat losses from recirculation systems and thediscarded warmth of waste water as it runs down the drain. Severaltechnologies are available that save energy (and water) by reducing theselosses or by passively recovering heat from wastewater streams and othersources. Energy savings from some individual technologies are reported tobe as much as 30 percent. Savings calculations of prototype systemsincluding bundles of technologies have been reported above 50 percent.This roundtable session will describe the current practices, summarizethe results of past and ongoing studies, discuss ways to think about hotwater system efficiency, and point to areas of future study. We will alsorecommend further steps to reduce unnecessary losses from hot waterdistribution systems.

Nitrification of archaeal ammonia oxidizers in a high- temperature hot spring

Science.gov (United States)

Chen, Shun; Peng, Xiaotong; Xu, Hengchao; Ta, Kaiwen

2016-04-01

The oxidation of ammonia by microbes has been shown to occur in diverse natural environments. However, the link of in situ nitrification activity to taxonomic identities of ammonia oxidizers in high-temperature environments remains poorly understood. Here, we studied in situ ammonia oxidation rates and the diversity of ammonia-oxidizing Archaea (AOA) in surface and bottom sediments at 77 °C in the Gongxiaoshe hot spring, Tengchong, Yunnan, China. The in situ ammonia oxidation rates measured by the 15N-NO3- pool dilution technique in the surface and bottom sediments were 4.80 and 5.30 nmol N g-1 h-1, respectively. Real-time quantitative polymerase chain reaction (qPCR) indicated that the archaeal 16S rRNA genes and amoA genes were present in the range of 0.128 to 1.96 × 108 and 2.75 to 9.80 × 105 gene copies g-1 sediment, respectively, while bacterial amoA was not detected. Phylogenetic analysis of 16S rRNA genes showed high sequence similarity to thermophilic Candidatus Nitrosocaldus yellowstonii, which represented the most abundant operational taxonomic units (OTU) in both surface and bottom sediments. The archaeal predominance was further supported by fluorescence in situ hybridization (FISH) visualization. The cell-specific rate of ammonia oxidation was estimated to range from 0.410 to 0.790 fmol N archaeal cell-1 h-1, higher than those in the two US Great Basin hot springs. These results suggest the importance of archaeal rather than bacterial ammonia oxidation in driving the nitrogen cycle in terrestrial geothermal environments.

Biofilm formation in a hot water system

DEFF Research Database (Denmark)

Bagh, L.K.; Albrechtsen, Hans-Jørgen; Arvin, Erik

2002-01-01

The biofilm formation rate was measured in situ in a hot water system in an apartment building by specially designed sampling equipment, and the net growth of the suspended bacteria was measured by incubation of water samples with the indigeneous bacteria. The biofilm formation rate reached......, in the sludge, or in the water from the distribution system was negligible. This indicated that bacterial growth took place on the inner surfaces in the hot water system and biofilm formation and detachment of bacteria could account for most of the suspended bacteria actually measured in hot water. Therefore...

Microbial Diversity, Distribution and Insight into Their Role in S, Fe and N Biogeochemical Cycling in the Hot Springs at Tengchong Geothermal Fields, Southwest China

Science.gov (United States)

Li, J.; Peng, X.; Zhang, L.

2014-12-01

Ten sediment samples collected from one acidic and three alkaline high temperature hot springs at Tengchong terrestrial geothermal field, Southwest China, were examined by the mineralogical, geochemical, and molecular biological techniques. The mineralogical and geochemical analyses suggested that these hot springs contain relative high concentrations of S, Fe and N chemical species. Specifically, the acidic hot spring was rich in Fe2+, SO42- and NH4+, while the alkaline hot springs were high in NO3-, H2S and S2O3-. Analyses of 16S rRNA sequences showed their bacterial communities were dominated by Aquificae, Cyanobacteria, Deinococci-Thermus, Firmicutes, Proteobacteria, and Thermodesulfobacteria, while the archeal clone libraries were dominated by Desulfurococcales, Sulfolobales, and Thermoproteales. Among them, the potential S-, N- and Fe-related oxidizing and reducing prokaryote were presenting as a relative high proportion but with a great difference in diversity and metabolic approaches of each sample. These findings provide some significant implications for the microbial function in element biogeochemical cycles within the Tengchong geothermal environments: i). the distinct differences in abundance and diversity of microbial communities of geothermal sediments were related to in situ different physicochemical conditions; ii). the S-, N- and Fe-related prokaryote would take advantage of the strong chemical disequilibria in the hot springs; iii). in return, their metabolic activities can promote the transformation of S, Fe and N chemical species, thus founded the bases of biogeochemical cycles in the terrestrial geothermal environments.

Phototrophs in high-iron-concentration microbial mats: physiological ecology of phototrophs in an iron-depositing hot spring

Science.gov (United States)

Pierson, B. K.; Parenteau, M. N.; Griffin, B. M.

1999-01-01

At Chocolate Pots Hot Springs in Yellowstone National Park the source waters have a pH near neutral, contain high concentrations of reduced iron, and lack sulfide. An iron formation that is associated with cyanobacterial mats is actively deposited. The uptake of [(14)C]bicarbonate was used to assess the impact of ferrous iron on photosynthesis in this environment. Photoautotrophy in some of the mats was stimulated by ferrous iron (1.0 mM). Microelectrodes were used to determine the impact of photosynthetic activity on the oxygen content and the pH in the mat and sediment microenvironments. Photosynthesis increased the oxygen concentration to 200% of air saturation levels in the top millimeter of the mats. The oxygen concentration decreased with depth and in the dark. Light-dependent increases in pH were observed. The penetration of light in the mats and in the sediments was determined. Visible radiation was rapidly attenuated in the top 2 mm of the iron-rich mats. Near-infrared radiation penetrated deeper. Iron was totally oxidized in the top few millimeters, but reduced iron was detected at greater depths. By increasing the pH and the oxygen concentration in the surface sediments, the cyanobacteria could potentially increase the rate of iron oxidation in situ. This high-iron-content hot spring provides a suitable model for studying the interactions of microbial photosynthesis and iron deposition and the role of photosynthesis in microbial iron cycling. This model may help clarify the potential role of photosynthesis in the deposition of Precambrian banded iron formations.

The distribution and abundance of archaeal tetraether lipids in U.S. Great Basin hot springs

Directory of Open Access Journals (Sweden)

Julienne J. eParaiso

2013-08-01

Full Text Available Isoprenoidal glycerol dialkyl glycerol tetraethers (iGDGTs are core membrane lipids of many archaea that enhance the integrity of cytoplasmic membranes in extreme environments. We examined the iGDGT profiles and corresponding aqueous geochemistry in 40 hot spring sediment and microbial mat samples from the U.S. Great Basin with temperatures ranging from 31 to 95°C and pH ranging from 6.8 to 10.7. The absolute abundance of iGDGTs correlated negatively with pH and positively with temperature. High lipid concentrations, distinct lipid profiles, and a strong relationship between polar and core lipids in hot spring samples suggested in situ production of most iGDGTs rather than contamination from local soils. Two-way cluster analysis and non-metric multidimensional scaling (NMS of polar iGDGTs indicated that the relative abundance of individual lipids was most strongly related to temperature (r2 = 0.546, with moderate correlations with pH (r2 = 0.359, nitrite (r2 = 0.286, oxygen (r2 = 0.259, and nitrate (r2 = 0.215. Relative abundance profiles of individual polar iGDGTs indicated potential temperature optima for iGDGT-0 (≤70°C, iGDGT-3 (≥55°C, and iGDGT -4 (≥60°C. These relationships likely reflect both physiological adaptations and community-level population shifts in response to temperature differences, such as a shift from cooler samples with more abundant methanogens to higher-temperature samples with more abundant Crenarchaeota. Crenarchaeol was widely distributed across the temperature gradient, which is consistent with other reports of abundant crenarchaeol in Great Basin hot springs and suggests a wide distribution for thermophilic ammonia-oxidizing archaea (AOA.

Water-Chemistry and On-Site Sulfur-Speciation Data for Selected Springs in Yellowstone National Park, Wyoming, 1996-1998

Science.gov (United States)

Ball, James W.; Nordstrom, D. Kirk; McCleskey, R. Blaine; Schoonen, Martin A.A.; Xu, Yong

2001-01-01

Fifty-eight water analyses are reported for samples collected from 19 hot springs and their overflow drainages and one ambient-temperature acid stream in Yellowstone National Park (YNP) during 1996-98. These water samples were collected and analyzed as part of research investigations on microbially mediated sulfur oxidation in stream waters and sulfur redox speciation in hot springs in YNP and chemical changes in overflow drainages that affect major ions, redox species, and trace elements. The research on sulfur redox speciation in hot springs is a collaboration with the State University of New York at Stony Brook, Northern Arizona University, and the U.S. Geological Survey (USGS). One ambient-temperature acidic stream system, Alluvium Creek and its tributaries in Brimstone Basin, was studied in detail. Analyses were performed adjacent to the sampling site, in an on-site mobile laboratory truck, or later in a USGS laboratory, depending on stability and preservability of the constituent. Water temperature, specific conductance, pH, Eh, dissolved oxygen (D.O.), and dissolved H2S were determined on-site at the time of sampling. Alkalinity and F were determined within a few days of sample collection by titration and by ion-selective electrode, respectively. Concentrations of S2O3 and SxO6 were determined as soon as possible (minutes to hours later) by ion chromatography (IC). Concentrations of Cl, SO4, and Br were determined by IC within a few days of sample collection. Concentrations of Fe(II) and Fe(total) were determined by ultraviolet/visible spectrophotometry within a few days of sample collection. Densities were determined later in the USGS laboratory. Concentrations of Li, Na, and K were determined by flame atomic absorption (Li) and emission (Na, K) spectrometry. Concentrations of Al, As(total), B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe(total), Mg, Mn, Ni, Pb, Si, Sr, V, and Zn were determined by inductively-coupled plasma optical emission spectrometry. Trace

Online analysis: Deeper insights into water quality dynamics in spring water.

Science.gov (United States)

Page, Rebecca M; Besmer, Michael D; Epting, Jannis; Sigrist, Jürg A; Hammes, Frederik; Huggenberger, Peter

2017-12-01

We have studied the dynamics of water quality in three karst springs taking advantage of new technological developments that enable high-resolution measurements of bacterial load (total cell concentration: TCC) as well as online measurements of abiotic parameters. We developed a novel data analysis approach, using self-organizing maps and non-linear projection methods, to approximate the TCC dynamics using the multivariate data sets of abiotic parameter time-series, thus providing a method that could be implemented in an online water quality management system for water suppliers. The (TCC) data, obtained over several months, provided a good basis to study the microbiological dynamics in detail. Alongside the TCC measurements, online abiotic parameter time-series, including spring discharge, turbidity, spectral absorption coefficient at 254nm (SAC254) and electrical conductivity, were obtained. High-density sampling over an extended period of time, i.e. every 45min for 3months, allowed a detailed analysis of the dynamics in karst spring water quality. Substantial increases in both the TCC and the abiotic parameters followed precipitation events in the catchment area. Differences between the parameter fluctuations were only apparent when analyzed at a high temporal scale. Spring discharge was always the first to react to precipitation events in the catchment area. Lag times between the onset of precipitation and a change in discharge varied between 0.2 and 6.7h, depending on the spring and event. TCC mostly reacted second or approximately concurrent with turbidity and SAC254, whereby the fastest observed reaction in the TCC time series occurred after 2.3h. The methodological approach described here enables a better understanding of bacterial dynamics in karst springs, which can be used to estimate risks and management options to avoid contamination of the drinking water. Copyright © 2017 Elsevier B.V. All rights reserved.

An Origin of Life in Cycling Hot Spring Pools: Emerging Evidence from Chemistry, Geology and Computational Studies

Science.gov (United States)

Deamer, D. W.; Damer, B. F.; Van Kranendonk, M. J.; Djokic, T.

2017-07-01

New evidence for an origin of life in a hot spring setting on land is supported by three studies: chemical (polymerization in wet-dry cycles), geological (stromatolites in a 3.48 Ga geothermal field) and computational (verifying the kinetic trap).

Balneological Evaluation of the Tafadek Spring, Agadez Region, Niger Republic

Science.gov (United States)

Nghargbu, K.; Latour, T.; Ponikowska, I.; Kurowska, E.

2012-04-01

West Africa, particularly Niger Republic is home to thousands of tourists annually. The vast Saharan desert and it's numerous oases in the northern parts of the Republic are a hot attraction on their own. However, in a recent survey of medicinal springs within the West African sub-region, the only hot spring in this country known locally for its therapeutic egress was analyzed. Located about 160km West of Agadez, the "Tafadek" spring with a yield of over 5l/s and temperature of about 50oC, rich in fluoride and silica is a specific water with capacity for application in balneotherapy for the treatment of orthopaedic, rheumatological, neurological, urinary tract infections, and osteoporosis. If the Tafadek spring is developed into a modern health resort promoting balneotherapy, health (balnear) tourism which is non-existent in Niger Republic today could kick start a new dawn in the health/economic development of the nation and the sub-region at large. Keywords: West Africa, Nigeria, Balneotherapy, Health tourism, Spring

Radioactivity measurement in spring waters of Cantabria, Spain

International Nuclear Information System (INIS)

Soto Torres, J.; Gomez Arozamena, J.

1999-01-01

A study of the radioactivity existing in a high number of springs located in Cantabria, Northern Spain, was made. The spring analyzed in three sampling campaign's, And alpha and beta total activities and 226 Ra and 222 Rn concentrations were determined for each sample. The measuring techniques employed were gamma spectrometry with Ge detector, counting with gas flow proportional counter, and counting with ZnS(Ag) scintillating detector. Results show that springs with high radon water concentration have high values respect to the national mean. The springs with the highest radium and radon levels have thermal waters and are located on two deep fault, those have historic seismicity and seismical and geomorphological evidences of recent tectonic activity

Direct use applications of geothermal resources at Desert Hot Springs, California. Final report, May 23, 1977--July 31, 1978. Volume II: appendixes

Energy Technology Data Exchange (ETDEWEB)

Christiansen, C.C.

1978-07-01

The following appendixes are included: Desert Hot Springs (DHS) Geothermal Project Advisory Board, Geothermal Citizens Advisory Committee, community needs assessment, geothermal resource characterization, a detailed discussion of the geothermal applications considered for DHS, space/water heating, agricultural operations, detailed analysis of a geothermal aquaculture facility, detailed discussion of proposed energy cascading systems for DHS, regulatory requirements, environmental impact assessment, resource management plan, and geothermal resources property rights and powers of cities to regulate indigenous geothermal resources and to finance construction of facilities for utilization of such resources. (MHR)

Xylanases of thermophilic bacteria from Icelandic hot springs

Energy Technology Data Exchange (ETDEWEB)

Pertulla, M; Raettoe, M; Viikari, L [VTT, Biotechnical Lab., Espoo (Finland); Kondradsdottir, M [Dept. of Biotechnology, Technological Inst. of Iceland, Reykjavik (Iceland); Kristjansson, J K [Dept. of Biotechnology, Technological Inst. of Iceland, Reykjavik (Iceland) Inst. of Biotechnology, Iceland Univ., Reykjavik (Iceland)

1993-02-01

Thermophilic, aerobic bacteria isolated from Icelandic hot springs were screened for xylanase activity. Of 97 strains tested, 14 were found to be xylanase positive. Xylanase activities up to 12 nkat/ml were produced by these strains in shake flasks on xylan medium. The xylanases of the two strains producing the highest activities (ITI 36 and ITI 283) were similar with respect to temperature and pH optima (80deg C and pH 8.0). Xylanase production of strain ITI 36 was found to be induced by xylan and xylose. Xylanase activity of 24 nkat/ml was obtained with this strain in a laboratory-scale-fermentor cultivation on xylose medium. [beta]-Xylosidase activity was also detected in the culture filtrate. The thermal half-life of ITI 36 xylanase was 24 h at 70deg C. The highest production of sugars from hydrolysis of beech xylan was obtained at 70deg C, although xylan depolymerization was detected even up to 90deg C. (orig.).

Spatial Characteristics of Geothermal Spring Temperatures and Discharge Rates in the Tatun Volcanic Area, Taiwan

Science.gov (United States)

Jang, C. S.; Liu, C. W.

2014-12-01

The Tatun volcanic area is the only potential volcanic geothermal region in the Taiwan island, and abundant in hot spring resources owing to stream water mixing with fumarolic gases. According to the Meinzer's classification, spring temperatures and discharge rates are the most important properties for characterizing spring classifications. This study attempted to spatially characterize spring temperatures and discharge rates in the Tatun volcanic area, Taiwanusing indicator kriging (IK). First, data on spring temperatures and discharge rates, which were collected from surveyed data of the Taipei City Government, were divided into high, moderate and low categories according to spring classification criteria, and the various categories were regarded as estimation thresholds. Then, IK was adopted to model occurrence probabilities of specified temperatures and discharge rates in springs, and to determine their classifications based on estimated probabilities. Finally, nine combinations were obtained from the classifications of temperatures and discharge rates in springs. Moreover, the combinations and features of spring water were spatially quantified according to seven sub-zones of spring utilization. A suitable and sustainable development strategy of the spring area was proposed in each sub-zone based on probability-based combinations and features of spring water.The research results reveal that the probability-based classifications using IK provide an excellent insight in exploring the uncertainty of spatial features in springs, and can provide Taiwanese government administrators with detailed information on sustainable spring utilization and conservation in the overexploited spring tourism areas. The sub-zones BT (Beitou), RXY (Rd. Xingyi), ZSL (Zhongshanlou) and LSK (Lengshuikeng) with high or moderate discharge rates are suitable to supply spring water for tourism hotels.Local natural hot springs should be planned in the sub-zones DBT (Dingbeitou), ZSL, XYK

Isotope hydrogeochemical characteristics of water bodies in Munigou, Sichuan

International Nuclear Information System (INIS)

Li Hongye; Yin Guan; Yang Junyi; Fan Xiao

2003-01-01

Munigou is a part of Huanglong -Jiuzhaigou national scenic in northern Sichuan province. By using isotope geochemistry technique of natural water to analyze the data of δ D, δ 18 O, tritium contents, deuterium excess parameters (d-excess) and element chemical analyses, we discusses the recharge, runoff, discharge and hydraulic relationship between the precipitation, terrain surface water, groundwater and main spring in the Munigou area. Pearl spring and Jadeite spring are major spring resources in this spot. Pearl spring is a hot spring that recharged by the precipitation that sourced from an elevation of more than 3700 m. The part of precipitation infiltrate underground and return the earth's surface after it exchanged quantity of heat with deep hot fluid. So the Pearl spring can be exploited as hot spring; The Jadeite spring is typical acidic karst water cold spring. The velocity of flow of the water in the Jadeite spring's aquifer is slower than the Pearl spring's and the water has long detained time. The spring water has a good quality and important economical value for natural mineral water; Most of runoff in Munigou recharged by precipitation and shallow groundwater. This recharge have seasonal variation and important for scenic spot. These discussed problems in article have far-reaching meaning on evaluation the exploited potential of the Pearl spring and Jadeite spring and is important to protecting and developing the precious natural resource in Munigou area. And provides reference for the similar researches too. (authors)

What's in the mud?: Water-rock-microbe interactions in thermal mudpots and springs

Science.gov (United States)

Dahlquist, G. R.; Cox, A. D.

2016-12-01

Limited aspects of mudpot geochemistry, mineralogy, and microbiology have been previously investigated in a total of 58 mudpots in Yellowstone National Park (YNP), Kamchatka, Iceland, Italy, Valles Caldera, New Mexico, Nicaragua, and the Stefanos hydrothermal crater, Greece (Allen and Day, 1935; Raymahashay, 1968; Shevenell, 1987; Bradley, 2005; Prokofeva, 2006; Bortnikova, 2007; Kaasalainen, 2012; Szynkiewicz, 2012; Hynek, 2013; Pol, 2014; Kanellopoulos, 2016). The composition of 35 mudpots was analyzed for aqueous geochemistry of filtrate and solid phase characterization. Here mudpots are defined as thermal features with viscosities between 5 and 100 centipoise at the approximate temperature of the mudpot, which was measured by an Ofite hand cranked viscometer. Analogous samples of nearby hot springs provide comparisons between mudpots and non-viscous thermal features. Aqueous geochemistry from mudpots was obtained by a novel two-step filtration process consisting of gravity prefiltration by a 100 or 50 micron trace metal cleaned polyethylene bag filter followed by syringe filtration with 0.8/0.2 Supor membrane filters. This filtered sample water was preserved and analyzed for water isotopes, major anions and cations, dissolved organic carbon, and trace metals. Mudpot meter readings show dissolved oxygen values ranging from below the detection limit of 0.156 to 22.5uM, pH values ranging from 1.41 to 6.08, and temperatures ranging from 64.8 to 92.5°C. Mudpots and turbid hot springs exhibited an inverse relationship between dissolved rare earth element concentrations and dissolved calcium concentrations (where calcium concentrations > 0.4mM). Mudpots altered existing surficial geology to form clays, primarily kaolinite, montmorillionite, and alunite. This hydrothermal alteration leaches metals, allowing mudpots to concentrate metals. DNA was extracted from mudpot solids and amplified with eukaryotic, bacterial, archaeal, and universal primers, which yielded only

Filamentous Morphology as a Means for Thermophilic Bacteria to Survive Steep Physical and Chemical Gradients in Yellowstone Hot Springs

Science.gov (United States)

Dong, Y.; Srivastava, V.; Bulone, V.; Keating, K. M.; Khetani, R. S.; Fields, C. J.; Inskeep, W.; Sanford, R. A.; Yau, P. M.; Imai, B. S.; Hernandez, A. G.; Wright, C.; Band, M.; Cann, I. K.; Ahrén, D.; Fouke, K. W.; Sivaguru, M.; Fried, G.; Fouke, B. W.

2017-12-01

The filamentous heat-loving bacterium Sulfurihydrogenibium yellowstonense makes up more than 90% of the microbial community that inhabits turbulent, dysoxic hot spring outflow channels (66-71°C, 6.2-6.5 pH, 0.5-0.75 m/s flow rate) at Mammoth Hot Spring in Yellowstone National Park. These environments contain abundantly available inorganic substrates (e.g., CO2, sulfide and thiosulfate) and are associated with extensive CaCO3 (travertine) precipitation driven in part by CO2 off-gassing. Evidence from integrated Meta-Omics analyses of DNA, RNA, and proteins (metagenomics, metatranscriptomics and metaproteomics) extracted from these S. yellowstonense-dominated communities have detected 1499 non-rRNA open reading frames (ORFs), their transcripts and cognate proteins. During chemoautotrophy and CO2 carbon fixation, chaperons facilitate enzymatic stability and functionalities under elevated temperature. High abundance transcripts and proteins for Type IV pili and exopolysaccharides (EPS) are consistent with S. yellowstonense forming strong (up to 0.5 m) intertwined microbial filaments (fettuccini streamers) composed of linked individual cells that withstand hydrodynamic shear forces and extremely rapid travertine mineralization. Their primary energy source is the oxidation of reduced sulfur (e.g., sulphide, sulfur or thiosulfate) and the simultaneous uptake of extremely low concentrations of dissolved O2 facilitated by bd-type cytochromes. Field observations indicate that the fettuccini microbial filaments build up ridged travertine platforms on the bottom of the springs, parallel to the water flow, where living filaments attach almost exclusively to the top of each ridge. This maximizes their access to miniscule amounts of dissolved oxygen, while optimizing their ability to rapidly form down-flow branched filaments and thus survive in these stressful environments that few other microbes can inhabit.

Effects of potential geothermal development in the Corwin Springs Known Geothermal Resources Area, Montana, on the thermal features of Yellowstone National Park. Water Resources Investigation

International Nuclear Information System (INIS)

Sorey, M.L.

1991-01-01

A two-year study by the U.S. Geological Survey, in collaboration with the National Park Service, Argonne National Laboratory, and Los Alamos National Laboratory was initiated in 1988 to determine the effects of potential geothermal development in the Corwin Springs Known Geothermal Resources Area (KGRA), Montana, on the thermal features of Yellowstone National Park. The study addressed three principal issues: (1) the sources of thermal water in the hot springs at Mammoth, La Duke, and Bear Creek; (2) the degree of subsurface connection between these areas; and (3) the effects of geothermal development in the Corwin Springs KGRA on the Park's thermal features. The authors investigations included, but were not limited to, geologic mapping, electrical geophysical surveys, chemical sampling and analyses of waters and rocks, determinations of the rates of discharge of various thermal springs, and hydrologic tracer tests

Near-infrared detection of ammonium minerals at Ivanhoe Hot Springs, Nevada

Science.gov (United States)

Krohn, M. D.

1986-01-01

Airborne Imaging Spectrometer (AIS) data were collected over the fossil hot spring deposit at Ivanhoe, Nevada in order to determine the surface distribution of NH4-bearing minerals. Laboratory studies show that NH4-bearing minerals have characteristic absorption features in the near-infrared (NIR). Ammonium-bearing feldspars and alunites were observed at the surface of Ivanhoe using a hand-held radiometer. However, first look analysis of the AIS images showed that the line was about 500 m east of its intended mark, and the vegetation cover was sufficiently dense to inhibit preliminary attempts at making relative reflectance images for detection of ammonium minerals.

In situ ecophysiology of Aigarchaeota from an oxic, hot-spring filamentous 'streamer' community

Science.gov (United States)

Beam, J.; Jay, Z.; Tringe, S. G.; Glavina del Rio, T.; Rusch, D.; Schmid, M.; Wagner, M.; Inskeep, W.

2014-12-01

The candidate phylum Aigarchaeota contains thermophilic archaea from terrestrial, subsurface, and marine geothermal ecosystems. The phylogeny and metabolic potential of Aigarchaeota has been deduced from several recent single-cell amplified genomes; however, an accurate description of their metabolism, potential ecological interactions, and role in biogeochemical cycling is lacking. Here we report possible ecological interactions and the in situ metabolism of an uncultivated lineage of Aigarchaeota from an oxic, terrestrial hot-spring filamentous 'streamer' community (Octopus Spring, pH = 8; T = 78 - 84 °C, Yellowstone National Park, Wyoming, USA). Fluorescence in situ hybridization (FISH) was combined with detailed genomic and transcriptomic reconstruction to elucidate the ecophysiological role of Aigarchaeota in these streamer communities. This novel population of Aigarchaeota are filamentous (~500 nm diameter by ~10-30 μm length), which is consistent with the morphology predicted by the presence and transcription of a single actin-encoding gene. Aigarchaeota filaments are intricately associated with other community members, which include both thermophilic bacteria and archaea. Metabolic reconstruction suggests that this aigarchaeon is an aerobic, chemoorganotroph. A single heme copper oxidase complex was identified in de novo genome assemblies, and was highly transcribed in environmental samples. Potential electron donors include acetate, fatty acids, sugars, peptides, and aromatic compounds. Transcripts related to genes specific to each of these potential electron donors were identified, indicating that this population of Aigarchaeota likely utilizes a broad range of reduced carbon substrates. Potential electron donors for this population may include extracellular polymeric substances produced by other microorganisms in close proximity. Flagellum genes were also highly transcribed, which suggests a potential mechanism for motility and/or cell-cell attachment

Report on achievements in fiscal 1973 in studies of technologies to develop and utilize resources and preserve national land. Study on hot water systems in geothermal areas; 1973 nendo chinetsu chiiki no nessuikei ni kansuru kenkyu seika chukan hokokusho

Energy Technology Data Exchange (ETDEWEB)

NONE

1977-03-01

It is important for geothermal energy to develop and utilize it in a rational manner. To achieve the objective, hot water systems must be studied comprehensively and elucidated from the standpoint of the systems as a whole. The present study, standing on this viewpoint, is intended to elucidate hot water systems and establish a survey method thereon. Fiscal 1973 has selected four areas (northern Hachimantai, southern Hachimantai, Onikubi and Kuju areas) as the model study fields, and used as the main field the Onikubi area, which clearly shows the structural catchment basin. Studies were performed in this area on hydraulic hot flow rates, isotopic geology, and reservoirs. In the hydraulic hot flow rate study, the amount of rainfall, amount of flowing water, and amount of hot spring water flow-out were observed continually. In the isotopic geology study, hydrogen in hot spring water and underground water, and composition of oxygen isotope were analyzed. Estimation was made from the result thereof on water balance, heat balance, and underground residence time. In the study of reservoirs, measurements were performed inside the wells, and estimation was made on locations and sizes of the reservoirs by surveying distribution of transformed minerals and cracks. (NEDO)

Domestic hot water storage: Balancing thermal and sanitary performance

International Nuclear Information System (INIS)

Armstrong, P.; Ager, D.; Thompson, I.; McCulloch, M.

2014-01-01

Thermal stratification within hot water tanks maximises the availability of stored energy and facilitates optimal use of both conventional and renewable energy sources. However, stratified tanks are also associated with the proliferation of pathogenic bacteria, such as Legionella, due to the hospitable temperatures that arise during operation. Sanitary measures, aimed at homogenising the temperature distribution throughout the tank, have been proposed; such measures reduce the effective energy storage capability that is otherwise available. Here we quantify the conflict that arises between thermodynamic performance and bacterial sterilisation within 10 real world systems. Whilst perfect stratification enhances the recovery of hot water and reduces heat losses, water samples revealed significant bacterial growth attributable to stratification (P<0.01). Temperature measurements indicated that users were exposed to potentially unsanitary water as a result. De-stratifying a system to sterilise bacteria led to a 19% reduction in effective hot water storage capability. Increasing the tank size to compensate for this loss would lead to an 11% increase in energy consumed through standing heat losses. Policymakers, seeking to utilise hot water tanks as demand response assets, should consider monitoring and control systems that prevent exposures to unsanitary hot water. - Highlights: • Domestic hot water tanks are a potential demand side asset for power networks. • A preference for bacterial growth in stratified hot water tanks has been observed. • Temperatures in base of electric hot water tanks hospitable to Legionella. • Potential exposures to unsanitary water observed. • De-stratifying a tank to sterilise leads to reduced energy storage capability

Prevalence and Genetic Diversity of Enterococcus faecalis Isolates from Mineral Water and Spring Water in China

Directory of Open Access Journals (Sweden)

Lei Wei

2017-06-01

Full Text Available Enterococcus faecalis is an important opportunistic pathogen which is frequently detected in mineral water and spring water for human consumption and causes human urinary tract infections, endocarditis and neonatal sepsis. The aim of this study was to determine the prevalence, virulence genes, antimicrobial resistance and genetic diversity of E. faecalis from mineral water and spring water in China. Of 314 water samples collected from January 2013 to January 2014, 48 samples (15.3% were contaminated E. faecalis. The highest contamination rate occurred in activated carbon filtered water of spring water (34.5%, followed by source water of spring water (32.3% and source water of mineral water (6.4%. The virulence gene test of 58 E. faecalis isolates showed that the detection rates of asa1, ace, cylA, gelE and hyl were 79.3, 39.7, 0, 100, 0%, respectively. All 58 E. faecalis isolates were not resistant to 12 kinds of antibiotics (penicillin, ampicillin, linezolid, quinupristin/dalfopristin, vancomycin, gentamicin, streptomycin, ciprofloxacin, levofloxacin, norfloxacin, nitrofurantoin, and tetracycline. Enterobacterial repetitive intergenic consensus-PCR classified 58 isolates and three reference strains into nine clusters with a similarity of 75%. This study is the first to investigate the prevalence of E. faecalis in mineral water and spring water in China. The results of this study suggested that spring water could be potential vehicles for transmission of E. faecalis.

Water Quality Evaluation of Spring Waters in Nsukka, Nigeria ...

African Journals Online (AJOL)

Water qualities of springs in their natural state are supposed to be clean and potable. Although, water quality is not a static condition it depends on the local geology and ecosystem, as well as human activities such as sewage dispersion, industrial pollution, use of water bodies as a heat sink, and overuse. The activities on ...

Visit to valuable water springs. 22. ; Kanazawa spring and springs at the mountain flank of Iwate volcano. Meisui wo tazunete. 22. ; Kanazawa shimizu to Iwate sanroku yusuigun

Energy Technology Data Exchange (ETDEWEB)

Itadera, K. (Kanagawa Hot Springs Research Institute, Kanagawa (Japan)); Shimano, Y. (Utsunomiya Bunsei Junior College, Tochigi (Japan))

1993-06-30

This paper describes the following matters on the springs at the mountain flank of Iwate volcano in Iwate Prefecture, with the Kanazawa spring as the main subject: The new and old Iwate volcanos have rock-bed flow deposits which resulted from mountain disintegration, distributed over their south, east and north flanks, and most of the spring water wells up in these areas; the south, east and north flanks have about 80 springs, about 30 springs, and about 10 springs, respectively; the number of springs and the water well-up scale show a trend of inverse proportion; the Kanazawa spring is a generic name of the several springs located on the north flank in the Kanazawa area; its main spring forms a spring pond with an area of about 100 m[sup 2] with a spring water temperature of about 11.5[degree]C, electric conductivity of 200 [mu] S/cm or higher, and a flow-out rate of 500 l/s or more; the Kanazawa spring is characterized by having as large total dissolved component amount as 170 mg/l or more and abundant amount of SO4[sup 2-] and Cl[sup -]; and the spring presents properties different from those in other springs. 10 refs., 5 figs., 1 tab.

16S RRNA Gene Analysis of Chlorate Reducing Thermophilic Bacteria From Local Hot Spring

OpenAIRE

Aminin, Agustina L. N; Katulistiwasari, Puri; Mulyani, Nies Suci

2011-01-01

Chlorates waste remediation by biological processes has been the object of current research. Strain CR, the chlorate reducing bacteria was isolated from Gedongsongo hot spring using minimal medium broth containing chlorates and acetate at 55oC. The determination of chlorate reduction from medium was carried out using turbidimetric method. CR isolate showed reducing ability 18% after four days of incubation. The phenotypic character of CR isolate including rod-shaped cells, gram-positive bacte...

Energy saving type area hot water supply system using heat of hot waste water from the sludge center as hot source for hot water; New energy rokko airando CITY. Surajjisenta karano onhaisuinetsu wo kyuyuyo netsugen ni riyosuru sho energy gata chiiki onsui kyokyu system

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-01-01

Heat source of area hot water supply system in Rokko island City (man-made island) is heat of combustion at the sludge center (sludge incineration plant) in this island. Dehydrated sludge cakes (230ton/day) brought from seven sewage disposal plants in Kobe City is combusted (850degC) in the fluid bed hearth. Combustion gas washed in the scrubber, hot waste water after the washing give heat into heat transfer water in the first heat exchanger. Temperature being 64degC in summer and about 50degC in winter, this heat transfer water is sent into the second heat exchanger at every condominium building throughout the pipe line system circulating in the area. At each home, gas heater and hot water supply devices fitted, additional combustion is not necessary in summer but is used according to demand in other seasons. This hot water supply service has been carried out since 1988 and at present has been used by 3600 homes. Amount of supplying hot water being about 3000cu.m/day, saving is calculated roughly as 60% of gas for hot water supply. Fee for this system is 1500/yen/month uniformly for each home. 14 figs.

Discharge, water quality, and native fish abundance in the Virgin River, Utah, Nevada, and Arizona, in support of Pah Tempe Springs discharge remediation efforts

Science.gov (United States)

Miller, Matthew P.; Lambert, Patrick M.; Hardy, Thomas B.

2014-01-01

Pah Tempe Springs discharge hot, saline, low dissolved-oxygen water to the Virgin River in southwestern Utah, which is transported downstream to Lake Mead and the Colorado River. The dissolved salts in the Virgin River negatively influence the suitability of this water for downstream agricultural, municipal, and industrial use. Therefore, various remediation scenarios to remove the salt load discharged from Pah Tempe Springs to the Virgin River are being considered. One concern about this load removal is the potential to impact the ecology of the Virgin River. Specifically, information is needed regarding possible impacts of Pah Tempe Springs remediation scenarios on the abundance, distribution, and survival of native fish in the Virgin River. Future efforts that aim to quantitatively assess how various remediation scenarios to reduce the load of dissolved salts from Pah Tempe Springs into the Virgin River may influence the abundance, distribution, and survival of native fish will require data on discharge, water quality, and native fish abundance. This report contains organized accessible discharge, water quality, and native fish abundance data sets from the Virgin River, documents the compilation of these data, and discusses approaches for quantifying relations between abiotic physical and chemical conditions, and fish abundance.

Update on Production Chemistry of the Roosevelt Hot Springs Reservoir

Energy Technology Data Exchange (ETDEWEB)

Simmons, Stuart; Kirby, Stefan; Allis, Rick; Moore, Joe; Fischer, Tobias

2018-02-12

Analyses of production fluids from the Roosevelt Hot Springs reservoir were acquired from well sampling campaigns in 2015 and 2016. The resulting data have been recalculated to reservoir conditions by correcting for effects of steam loss, and the values are compared to legacy data from earlier reports to quantify changes with time in response to fluid production. The reservoir composition is similar to that at the start of reservoir exploitation, having near neutral pH, total dissolved solids of 7000-10,000 mg/kg, and ionic ratios of Cl/HCO3 ~50-100, Cl/SO4 ~50-100, and Na/K ~4-5. Cation, gas and silica geothermometers indicate a range of equilibration temperatures between 240 and 300 °C, but quartz-silica values are most closely consistent with measured reservoir temperatures and well enthalpies. The largest change in fluid composition is observed in well 54-3. The fluid has evolved from being fed by a single phase liquid to a twophase mixture of steam and liquid due to pressure draw down. The fluid also shows a 25% increase in reservoir chloride and a ~20° C decrement of cooling related to mixing with injected brine. The other production wells also show increase in chloride and decrease in temperature, but these changes diminish in magnitude with distance from injection well 14-2. Stable isotope compositions indicate that the reservoir water is largely meteoric in origin, having been modified by hydrothermal waterrock interaction. The water has also become progressively enriched in isotopic values in response to steam loss and mixing of injectate. N2-Ar-He and helium isotope ratios indicate a deep magmatic source region that probably supplies the heat for the hydrothermal system, consistent with recent Quaternary volcanism in the Mineral Mountains.

Hot spring therapy of atomic bomb exposed patients, (9)

Energy Technology Data Exchange (ETDEWEB)

Hatta, O [Balneogic Sanatorium for the Atomic Bomb Injured Beppu, Oita (Japan); Tsuji, H

1978-03-01

The following description shows the statistics and the results of medical examinatin concerning the patients utilized Beppu Atomic Bomb Center from April, 1977, to March, 1978. Number of persons utilized the center was 3904, and 20285 man-days in total. Number of case treated there was 268. Number of diseases amounted to 442 of 66 sorts, excluding temporary of acute diseases such as acute entergastritis and cold diseases, etc. According to the report by the Ministry of Health and Welfare, atomic bomb-exposed persons show twice as much rate of incidence as normal persons, and owing to aging, many of them have more than two kinds of diseases. Among the diseases, 60 cases were hypertension, 32 heart disease, 30 knee-arthritis, 26 diabetes, 25 hepatitis, 23 spondylosis deformans, etc. Among 268 cases treated by hot spring therapy, 6 were totally cured, and 252 showed alleviation, while 10 showed no change.

Thermoanaerobacter mathranii sp. nov., an ethanol-producing, extremely thermophilic anaerobic bacterium from a hot spring in Iceland

DEFF Research Database (Denmark)

Larsen, L.; Nielsen, P.; Ahring, B.K.

1997-01-01

The extremely thermophilic ethanol-producing strain A3 was isolated from a hot spring in Iceland, The cells were rod-shaped, motile, and had terminal spores: cells from the mid-to-late exponential growth phase stained gram-variable but had a gram-positive cell wall structure when viewed...

Future Availability of Water Supply from Karstic Springs under Probable Climate Change. The case of Aravissos, Central Macedonia, Greece.

Science.gov (United States)

Vafeiadis, M.; Spachos, Th.; Zampetoglou, K.; Soupilas, Th.

2012-04-01

The test site of Aravissos is located at 70 Km to the West (W-NW) of Thessaloniki at the south banks of mount Païko, in the north part of Central Macedonia The karstic Aravissos springs supply 40% of total volume needed for the water supply of Thessaloniki, Greece. As the water is of excellent quality, it is feed directly in the distribution network without any previous treatment. The availability of this source is therefore of high importance for the sustainable water supply of this area with almost 1000000 inhabitants. The water system of Aravissos is developed in a karstic limestone with an age of about Late Cretaceous that covers almost the entire western part of the big-anticline of Païko Mountain. The climate in this area and the water consumption area, Thessaloniki, is a typical Mediterranean climate with mild and humid winters and hot and dry summers. The total annual number of rainy days is around 110. The production of the Aravissos springs depends mostly from the annual precipitations. As the feeding catchement and the karst aquifer are not well defined, a practical empirical balance model, that contains only well known relevant terms, is applied for the simulation of the operation of the springs under normal water extraction for water supply in present time. The estimation of future weather conditions are based on GCM and RCM simulation data and the extension of trend lines of the actual data. The future evolution of the availability of adequate water quantities from the springs is finally estimated from the balance model and the simulated future climatic data. This study has been realised within the project CC-WaterS, funded by the SEE program of the European Regional Development Fund (http://www.ccwaters.eu/).

BC SEA Solar Hot Water Acceleration project

Energy Technology Data Exchange (ETDEWEB)

Harris, N.C. [BC Sustainable Energy Association, Victoria, BC (Canada)

2005-07-01

Although solar hot water heating is an environmentally responsible technology that reduces fossil fuel consumption and helps mitigate global climate change, there are many barriers to its widespread use. Each year, domestic water heating contributes nearly 6 million tonnes of carbon dioxide towards Canada's greenhouse gas emissions. The installation of solar water heaters can eliminate up to 2 tonnes of carbon dioxide emissions per household. The BC SEA Solar Hot Water Acceleration project was launched in an effort to demonstrate that the technology has the potential to be widely used in homes and businesses across British Columbia. One of the main barriers to the widespread use of solar hot water heating is the initial cost of the system. Lack of public awareness and understanding of the technology are other barriers. However, other jurisdictions around the world have demonstrated that the use of renewables are the product of conscious policy decisions, including low-cost financing and other subsidies that have created demand for these technologies. To this end, the BC SEA Solar Hot Water Acceleration project will test the potential for the rapid acceleration of solar water heating in pilot communities where barriers are removed. The objective of the project is to install 100 solar water systems in homes and 25 in businesses and institutions in communities in British Columbia by July 2007. The project will explore the financial barriers to the installation of solar hot water systems and produce an action plan to reduce these barriers. In addition to leading by example, the project will help the solar energy marketplace, mitigate climate change and improve energy efficiency.

Heat losses through pipe connections in hot water stores

DEFF Research Database (Denmark)

Andersen, Elsa; Fan, Jianhua; Furbo, Simon

2007-01-01

The heat loss from pipe connections at the top of hot water storage tanks with and without a heat trap is investigated theoretically and compared to similar experimental investigations. Computational Fluid Dynamics (CFD) is used for the theoretical analysis. The investigations show that the heat...... loss from an ideally insulated pipe connected to the top of a hot water tank is mainly due to a natural convection flow in the pipe, that the heat loss coefficient of pipes connected to the top of a hot water tank is high, and that a heat trap can reduce the heat loss coefficient significantly. Further......, calculations show that the yearly thermal performance of solar domestic hot water systems is strongly reduced if the hot water tank has a thermal bridge located at the top of the tank....

The Origin of Carbon-bearing Volatiles in Surprise Valley Hot Springs in the Great Basin: Carbon Isotope and Water Chemistry Characterizations

Science.gov (United States)

Fu, Qi; Socki, Richard A.; Niles, Paul B.; Romanek, Christopher; Datta, Saugata; Darnell, Mike; Bissada, Adry K.

2013-01-01

There are numerous hydrothermal fields within the Great Basin of North America, some of which have been exploited for geothermal resources. With methane and other carbon-bearing compounds being observed, in some cases with high concentrations, however, their origins and formation conditions remain unknown. Thus, studying hydrothermal springs in this area provides us an opportunity to expand our knowledge of subsurface (bio)chemical processes that generate organic compounds in hydrothermal systems, and aid in future development and exploration of potential energy resources as well. While isotope measurement has long been used for recognition of their origins, there are several secondary processes that may generate variations in isotopic compositions: oxidation, re-equilibration of methane and other alkanes with CO2, mixing with compounds of other sources, etc. Therefore, in addition to isotopic analysis, other evidence, including water chemistry and rock compositions, are necessary to identify volatile compounds of different sources. Surprise Valley Hot Springs (SVHS, 41 deg 32'N, 120 deg 5'W), located in a typical basin and range province valley in northeastern California, is a terrestrial hydrothermal spring system of the Great Basin. Previous geophysical studies indicated the presence of clay-rich volcanic and sedimentary rocks of Tertiary age beneath the lava flows in late Tertiary and Quaternary. Water and gas samples were collected for a variety of chemical and isotope composition analyses, including in-situ pH, alkalinity, conductivity, oxidation reduction potential (ORP), major and trace elements, and C and H isotope measurements. Fluids issuing from SVHS can be classified as Na-(Cl)-SO4 type, with the major cation and anion being Na+ and SO4(2-), respectively. Thermodynamic calculation using ORP and major element data indicated that sulfate is the most dominant sulfur species, which is consistent with anion analysis results. Aquifer temperatures at depth

Discharge, water temperature, and water quality of Warm Mineral Springs, Sarasota County, Florida: A retrospective analysis

Science.gov (United States)

Metz, Patricia A.

2016-09-27

Warm Mineral Springs, located in southern Sarasota County, Florida, is a warm, highly mineralized, inland spring. Since 1946, a bathing spa has been in operation at the spring, attracting vacationers and health enthusiasts. During the winter months, the warm water attracts manatees to the adjoining spring run and provides vital habitat for these mammals. Well-preserved late Pleistocene to early Holocene-age human and animal bones, artifacts, and plant remains have been found in and around the spring, and indicate the surrounding sinkhole formed more than 12,000 years ago. The spring is a multiuse resource of hydrologic importance, ecological and archeological significance, and economic value to the community.The pool of Warm Mineral Springs has a circular shape that reflects its origin as a sinkhole. The pool measures about 240 feet in diameter at the surface and has a maximum depth of about 205 feet. The sinkhole developed in the sand, clay, and dolostone of the Arcadia Formation of the Miocene-age to Oligocene-age Hawthorn Group. Underlying the Hawthorn Group are Oligocene-age to Eocene-age limestones and dolostones, including the Suwannee Limestone, Ocala Limestone, and Avon Park Formation. Mineralized groundwater, under artesian pressure in the underlying aquifers, fills the remnant sink, and the overflow discharges into Warm Mineral Springs Creek, to Salt Creek, and subsequently into the Myakka River. Aquifers described in the vicinity of Warm Mineral Springs include the surficial aquifer system, the intermediate aquifer system within the Hawthorn Group, and the Upper Floridan aquifer in the Suwannee Limestone, Ocala Limestone, and Avon Park Formation. The Hawthorn Group acts as an upper confining unit of the Upper Floridan aquifer.Groundwater flow paths are inferred from the configuration of the potentiometric surface of the Upper Floridan aquifer for September 2010. Groundwater flow models indicate the downward flow of water into the Upper Floridan aquifer

[Abundances of ammonia-oxidizing archaeal accA and amoA genes in response to NO2 - and NO3 - of hot springs in Yunnan province].

Science.gov (United States)

Song, Zhaoqi; Wang, Li; Zhou, Enmin; Wang, Fengping; Xiao, Xiang; Zhang, Chuanlun; Li, Wenjun

2014-12-04

Yunnan hot springs have highly diverseammonia-oxidizing archaea (AOA), which are autotrophic and can fix CO2 using the 3-hydroxypropionate/ 4-hydroxybutyrate (HP/HD) pathway. In this study, we investigated the abundances of prokaryotic 16S rRNA gene and archaeal accA and amoA genes in the sediments of hot springs of Yunnan Province, and analysed the correlations between the above gene abundances and environmental factors. We selected the sediments of twenty representative hot springs, and detected the gene abundances by quantitative polymerase chain reaction (qPCR). The principal component analysis (PCA) and the Mantel test in the R software package were performed for the correlations of gene abundance and environmental variables. The bacterial and archaeal 16S rRNA gene abundances were from 6.6 x 10(7) to 4.19 x 10(11) and from 1.27 x 10(6) to 1.51 x 10(11) copies/g sediment, respectively; Archaeal accA and amoA genes were from 8.89 x 10(3) to 6.49 x 10(5) and from 7.64 x 10(3) to 4.36 x 10(5) copies/g sediment, respectively. The results of mantel test showed that accA gene was significantly (R = 0.98, P < 0.001) correlated with amoA gene; Both of them also were correlated significantly with NO2- and NO3 -, but not with pH. The abundances of bacterial and archaeal 16S rRNA genes and the ratio between them varied significantly among Yunnan hot springs. The archaealaccA and amoA genes showed significant correlation with each other, validating our previous finding that AOA in terrestrial hot springs might acquire energy from ammonia oxidation coupled with CO2 fixation using the 3-hydroxypropionate/4-hydroxybutyrate pathway.

Basics of Solar Heating & Hot Water Systems.

Science.gov (United States)

American Inst. of Architects, Washington, DC.

In presenting the basics of solar heating and hot water systems, this publication is organized from the general to the specific. It begins by presenting functional and operational descriptions of solar heating and domestic hot water systems, outlining the basic concepts and terminology. This is followed by a description of solar energy utilization…

Long term consumption of mineral spring water containing natural radium-226

International Nuclear Information System (INIS)

Aulenbach, D.B.; Davis, R.E.

1976-01-01

The presence of naturally occurring 226 radium in several of the spring waters of Saratoga Springs, New York has been known for some time. However, recently the recommended maximum acceptable limit for consumption of water containing radium has been lowered to the point that the limits are now lower than the concentration of radium observed in several of the wells. A survey was made of 27 individuals who have consumed water from the Hathorn No. 1 Spring for periods varying from 5-65 years. A calculation was made of the 226 radium body burden from equations provided in the literature. The calcium concentration of the springs was determined in consideration of the still unknown comparative selectivity of the body between radium and calcium. Waters from two of the springs were analzyed for 226 radium using the radon emanation method. No adverse effects of consuming the mineral water were observed in the individuals interviewed nor were there any increased incidences of broken or brittle bones among these individuals

Uranium in spring water and bryophytes at Basin Creek in central Idaho

International Nuclear Information System (INIS)

Shacklette, H.T.; Erdman, J.A.

1982-01-01

Arkosic sandstones and conglomerates of Tertiary age beneath the Challis Volcanics of Eocene age at Basin Creek, 10 km northeast of Stanley, Idaho, contain uranium-bearing vitrainized carbon fragments. The economic potential of these sandstones and conglomerates is currently being assessed. Water from 22 springs and associated bryophytes were sampled; two springs were found to contain apparently anomalous concentrations (normalized) of uranium. Water from a third spring contained slightly anomalous amounts of uranium, and two species of mosses at the spring contained anomalous uranium and high levels of both cadmium and lead. Water from a fourth spring was normal for uranium, but the moss from the water contained a moderate uranium level and highly anomalous concentrations of lead, germanium, and thallium. These results suggest that, in the Basin Creek area, moss sampling at springs may give a more reliable indication of uranium occurrence than would water sampling. (Auth.)

Solar Energy for Space Heating & Hot Water.

Science.gov (United States)

Energy Research and Development Administration, Washington, DC. Div. of Solar Energy.

This pamphlet reviews the direct transfer of solar energy into heat, particularly for the purpose of providing space and hot water heating needs. Owners of buildings and homes are provided with a basic understanding of solar heating and hot water systems: what they are, how they perform, the energy savings possible, and the cost factors involved.…

46 CFR 63.25-3 - Electric hot water supply boilers.

Science.gov (United States)

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Electric hot water supply boilers. 63.25-3 Section 63.25... water supply boilers. (a) Electric hot water supply boilers that have a capacity not greater than 454... section except the periodic testing required by paragraph (j) of this section. Electric hot water supply...

Do natural spring waters in Australia and New Zealand affect health? A systematic review.

Science.gov (United States)

Stanhope, Jessica; Weinstein, Philip; Cook, Angus

2018-02-01

Therapeutic use of spring waters has a recorded history dating back to at least 1550 BC and includes both bathing in and drinking such waters for their healing properties. In Australia and New Zealand the use of therapeutic spring waters is a much more recent phenomenon, becoming a source of health tourism from the late 1800s. We conducted a systematic review aimed at determining the potential health outcomes relating to exposure to Australian or New Zealand natural spring water. We found only low-level evidence of adverse health outcomes relating to this spring water exposure, including fatalities from hydrogen sulphide poisoning, drowning and primary amoebic meningoencephalitis. We found no studies that investigated the therapeutic use of these waters, compared with similar treatment with other types of water. From the broader literature, recommendations have been made, including fencing potentially harmful spring water, and having signage and media messages to highlight the potential harms from spring water exposure and how to mitigate the risks (e.g. not putting your head under water from geothermal springs). Sound research into the potential health benefits of Australian and New Zealand spring waters could provide an evidence base for the growing wellness tourism industry.

Water-chemistry data for selected springs, geysers, and streams in Yellowstone National Park, Wyoming, 2006-2008

Science.gov (United States)

Ball, James W.; McMleskey, R. Blaine; Nordstrom, D. Kirk

2010-01-01

Water analyses are reported for 104 samples collected from numerous thermal and non-thermal features in Yellowstone National Park (YNP) during 2006-2008. Water samples were collected and analyzed for major and trace constituents from 10 areas of YNP including Apollinaris Spring and Nymphy Creek along the Norris-Mammoth corridor, Beryl Spring in Gibbon Canyon, Norris Geyser Basin, Lower Geyser Basin, Crater Hills, the Geyser Springs Group, Nez Perce Creek, Rabbit Creek, the Mud Volcano area, and Washburn Hot Springs. These water samples were collected and analyzed as part of research investigations in YNP on arsenic, antimony, iron, nitrogen, and sulfur redox species in hot springs and overflow drainages, and the occurrence and distribution of dissolved mercury. Most samples were analyzed for major cations and anions, trace metals, redox species of antimony, arsenic, iron, nitrogen, and sulfur, and isotopes of hydrogen and oxygen. Analyses were performed at the sampling site, in an on-site mobile laboratory vehicle, or later in a U.S. Geological Survey laboratory, depending on stability of the constituent and whether it could be preserved effectively. Water samples were filtered and preserved on-site. Water temperature, specific conductance, pH, emf (electromotive force or electrical potential), and dissolved hydrogen sulfide were measured on-site at the time of sampling. Dissolved hydrogen sulfide was measured a few to several hours after sample collection by ion-specific electrode on samples preserved on-site. Acidity was determined by titration, usually within a few days of sample collection. Alkalinity was determined by titration within 1 to 2 weeks of sample collection. Concentrations of thiosulfate and polythionate were determined as soon as possible (generally a few to several hours after sample collection) by ion chromatography in an on-site mobile laboratory vehicle. Total dissolved iron and ferrous iron concentrations often were measured on-site in the

Metagenomic analysis of bacterial diversity of Siloam hot water ...

African Journals Online (AJOL)

use

2011-12-07

Dec 7, 2011 ... 14.40. Bicarbonate (HCO3). 107.36. Table 3. Trace elements composition at Siloam Hot spring. Element. Concentration (μg/L) Element. Concentration (μg/L) Element. Concentration μg/L. Antimony. 0.0. Cobalt. 0.1. Strontium. 20.4. Arsenic. 0.3. Copper. 0.0. Tellurium. 0.0. Barium. 4.2. Iodine. 1.9. Thallium.

Chemical and Hydro-Geologic Analysis of Ikogosi Warm Spring Water in Nigeria

OpenAIRE

Akinola Ikudayisi; Folasade Adeyemo; Josiah Adeyemo

2015-01-01

This study focuses on the hydro-geology and chemical constituents analysis of Ikogosi Warm Spring waters in South West Nigeria. Ikogosi warm spring is a global tourist attraction because it has both warm and cold spring sources. Water samples from the cold spring, warm spring and the meeting point were collected, analyzed and the result shows close similarity in temperature, hydrogen iron concentration (pH), alkalinity, hardness, Calcium, Magnesium, Sodium, Iron, total di...

Upgrading of biomass by carbonization in hot compressed water

Directory of Open Access Journals (Sweden)

Wiwut Tanthapanichakoon

2006-09-01

Full Text Available Carbonization of biomass (corn cob in hot compressed water was performed using a small bomb reactor at temperature 300-350ºC and pressure 10-18 MPa for 30 min. Then, the solid product or biochar was subjected to various analyses in order to investigate the effects of the carbonization in hot compressed water on the characteristics of the biochar. It was found that the yield of biochar carbonized in hot compressed water at 350ºC and pressure of 10 MPa for 30 min was 44.7%, whereas the yield of biochar carbonized in nitrogen atmosphere at 350ºC is 36.4%. Based on the information obtained from the elemental analyses of the biochar, it was found that the oxygen functional groups in the corn cob were selectively decomposed during the carbonization in hot compressed water. The pyrolysis and combustion behaviors of the biochar were found to be affected significantly by the carbonization in hot compressed water.

HEAT LOSS FROM HOT WATER SUPPLY LINE IN A RESIDENTIAL BUILDING

OpenAIRE

近藤, 修平; 鉾井, 修一

2011-01-01

In order to the evaluate heat loss from hot water supply lines in a residential building, hot water demand in a house in Chiba prefecture was measured and analyzed. The following results were obtained. 1. The heat loss of the hot water supply line was about 132kJ for the shower and 110kJ for the bathtub in winter. Since the temperature difference between the inlet and outlet of the hot water supply line is small, the measured heat loss from the hot water supply line sometimes becomes negative...

Domestic Hot Water Usage in Hotels; Tappvarmvattenanvaendning paa hotell

Energy Technology Data Exchange (ETDEWEB)

Petersson, Stefan; Werner, Sven [FVB Sverige AB, Vaesteraas (Sweden); Sandberg, Martin; Wahlstroem, Aasa [Swedish National Testing and Research Inst., Boraas (Sweden)

2004-06-01

Historically, design curves for domestic hot water, have been well sized and therefore also the components oversized. The Swedish district heating companies have noticed this and some companies replace large valves with customer-required valves, which give several advantages. There are several reasons why valves and heat exchangers can be customer-required and still the customers demand for hot water comfort can be fulfilled. The domestic hot water flow is composed, the taps are often short, large simultaneous taps are not very likely. Also, the dimensioning flows occur in the winter period, while the components are dimensioned for the summer case. The water pipes level off temporary temperature drops and the user seldom notices these because water with 55 deg C is not used in the tap. For residential buildings there are dimensioning recommendations on domestic hot water flow, but not for hotels. The purpose of this project has been to evaluate the domestic hot water use in relation to size and number of occupied beds. If the patterns of the chosen hotels coincide regarding to the sizes, dimensioning curves for domestic hot water use can be suggested. They can be used when hotels, or buildings with the same use pattern, are being built or restored. Measurements on 3 hotels with different sizes have been made. The hotels have 36, 52 and 158 rooms. The hotels are situated in the cities of Boraas and Kinna in Sweden. A short period of measurements from another hotel in the city of Gaevle (199 rooms) has also been included in this project. The measurements show that large hot water taps in hotels are rare and short. For the hotels, relative, cumulative relative frequencies and likely extreme values have been estimated. For residential buildings, The Swedish District Heating Association have recommendations for dimensioned domestic hot water flows. Formerly, these recommendations have been levelled so a cumulative relative frequency of 1 %, is reached, i.e. 99 % of all

Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library

Directory of Open Access Journals (Sweden)

Wei Yan

2017-03-01

Full Text Available A new gene encoding a lipase (designated as Lip-1 was identified from a metagenomic bacterial artificial chromosome(BAC library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622 amino acid residues. It was cloned, fused with the oleosin gene and over expressed in Escherichia coli to prepare immobilized lipase artificial oil body AOB-sole-lip-1. The monomeric Sole-lip-1 fusion protein presented a molecular mass of 102.4 kDa. Enzyme assays using olive oil and methanol as the substrates in petroleum ether confirmed its transesterification activity. Hexadecanoic acid methyl ester, 8,11-Octadecadienoic acid methyl ester, 8-Octadecenoic acid methyl ester, and Octadecanoic acid methyl ester were detected. It showed favorable transesterification activity with optimal temperature 45 °C. Besides, the maximal biodiesel yield was obtained when the petroleum ether system as the organic solvent and the substrate methanol in 350 mmol/L (at a molar ratio of methanol of 10.5:1 and the water content was 1%. In light of these advantages, this lipase presents a promising resource for biodiesel production.

Geochemical and physical drivers of microbial community structure in hot spring ecosystems

Science.gov (United States)

Havig, J. R.; Hamilton, T. L.; Boyd, E. S.; Meyer-Dombard, D. R.; Shock, E.

2012-12-01

Microbial communities in natural systems are typically characterized using samples collected from a single time point, thereby neglecting the temporal dynamics that characterize natural systems. The composition of these communities obtained from single point samples is then related to the geochemistry and physical parameters of the environment. Since most microbial life is adapted to a relatively narrow ecological niche (multiplicity of physical and chemical parameters that characterize a local habitat), these assessments provide only modest insight into the controls on community composition. Temporal variation in temperature or geochemical composition would be expected to add another dimension to the complexity of niche space available to support microbial diversity, with systems that experience greater variation supporting a greater biodiversity until a point where the variability is too extreme. . Hot springs often exhibit significant temporal variation, both in physical as well as chemical characteristics. This is a result of subsurface processes including boiling, phase separation, and differential mixing of liquid and vapor phase constituents. These characteristics of geothermal systems, which vary significantly over short periods of time, provide ideal natural laboratories for investigating how i) the extent of microbial community biodiversity and ii) the composition of those communities are shaped by temporal fluctuations in geochemistry. Geochemical and molecular samples were collected from 17 temporally variable hot springs across Yellowstone National Park, Wyoming. Temperature measurements using data-logging thermocouples, allowing accurate determination of temperature maximums, minimums, and ranges for each collection site, were collected in parallel, along with multiple geochemical characterizations as conditions varied. There were significant variations in temperature maxima (54.5 to 90.5°C), minima (12.5 to 82.5°C), and range (3.5 to 77.5°C) for

Metagenomics of Kamchatkan hot spring filaments reveal two new major (hyper)thermophilic lineages related to Thaumarchaeota.

Science.gov (United States)

Eme, Laura; Reigstad, Laila J; Spang, Anja; Lanzén, Anders; Weinmaier, Thomas; Rattei, Thomas; Schleper, Christa; Brochier-Armanet, Céline

2013-06-01

Based on phylogenetic analyses and gene distribution patterns of a few complete genomes, a new distinct phylum within the Archaea, the Thaumarchaeota, has recently been proposed. Here we present analyses of six archaeal fosmid sequences derived from a microbial hot spring community in Kamchatka. The phylogenetic analysis of informational components (ribosomal RNAs and proteins) reveals two major (hyper-)thermophilic clades ("Hot Thaumarchaeota-related Clade" 1 and 2, HTC1 and HTC2) related to Thaumarchaeota, representing either deep branches of this phylum or a new archaeal phylum and provides information regarding the ancient evolution of Archaea and their evolutionary links with Eukaryotes. Copyright © 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Modeling patterns of hot water use in households

Energy Technology Data Exchange (ETDEWEB)

Lutz, James D.; Liu, Xiaomin; McMahon, James E.; Dunham, Camilla; Shown, Leslie J.; McCure, Quandra T.

1996-01-01

This report presents a detailed model of hot water use patterns in individual households. The model improves upon an existing model by including the effects of four conditions that were previously unaccounted for: the absence of a clothes washer; the absence of a dishwasher; a household consisting of seniors only; and a household that does not pay for its own hot water use. Although these four conditions can significantly affect residential hot water use, and have been noted in other studies, this is the first time that they have been incorporated into a detailed model. This model allows detailed evaluation of the impact of potential efficiency standards for water heaters and other market transformation policies.

Hydrogeology and water chemistry of Infranz catchment springs, Bahir Dar Area, Lake Tana Basin, Ethiopia

Science.gov (United States)

Abera, F. N.

2017-12-01

The major springs in the Infranz catchment are a significant source of water for Bahir city and nearby villages, while they help to sustain Infranz River and the downstream wetlands. The aim of the research was to understand the hydrogeological conditions of these high-discharge springs, and to explain the hydrochemical composition of spring waters. Water samples from rainwater and springs were collected and analyzed and compared for major cations and anions. The hydrochemical data analysis showed that all water samples of the springs have freshwater chemistry, Ca-HCO3 type, while deep groundwater shows more evolved types. This indicates limited water-rock interaction and short residence time for the spring waters. The rise of NO3- and PO43- may indicate future water quality degradation unless the anthropogenic activities upgradient and nearby are restricted. The uptake of 75% of spring water for water supply of Bahir Dar results in wetland degradation. Key words: Spring water, Infranz River, Bahir Dar, Ethiopia, hydrochemistry

Genome Sequence of Anoxybacillus flavithermus Strain AK1, a Thermophile Isolated from a Hot Spring in Saudi Arabia

KAUST Repository

Khalil, Amjad

2015-06-04

Anoxybacillus flavithermus strain AK1 was isolated from Al-Ain Alhara, a thermal hot spring located 50 km southeast of the city of Gazan, Saudi Arabia (16°56ʹN, 43°15ʹE). The sequenced and annotated genome is 2,630,664 bp and encodes 2,799 genes.

Genome Sequence of Anoxybacillus flavithermus Strain AK1, a Thermophile Isolated from a Hot Spring in Saudi Arabia

KAUST Repository

Khalil, Amjad; Neelamegam, Sivakumar; Alqarawi, Sami

2015-01-01

Anoxybacillus flavithermus strain AK1 was isolated from Al-Ain Alhara, a thermal hot spring located 50 km southeast of the city of Gazan, Saudi Arabia (16°56ʹN, 43°15ʹE). The sequenced and annotated genome is 2,630,664 bp and encodes 2,799 genes.

Structural and Functional Insights from the Metagenome of an Acidic Hot Spring Microbial Planktonic Community in the Colombian Andes

NARCIS (Netherlands)

Jiménez Avella, Diego; Dini Andreote, Fernando; Chaves, Diego; Montaña, José Salvador; Osorio-Forero, Cesar; Junca, Howard; Zambrano, María Mercedes; Baena, Sandra

2012-01-01

A taxonomic and annotated functional description of microbial life was deduced from 53 Mb of metagenomic sequence retrieved from a planktonic fraction of the Neotropical high Andean (3,973 meters above sea level) acidic hot spring El Coquito (EC). A classification of unassembled metagenomic reads

The Origin of Carbon-bearing Volatiles in Surprise Valley Hot Springs in the Great Basin: Carbon Isotope aud Water Chemistry Characterizations

Science.gov (United States)

Fu, Qi; Socki, Richard A.; Niles, Paul B.; Romanek, Christopher; Datta, Saugata; Darnell, Mike; Bissada, Adry K.

2013-01-01

There are numerous hydrothermal fields within the Great Basin of North America, some of which have been exploited for geothermal resources. With methane and other carbon-bearing compounds being observed, in some cases with high concentrations, however, their origins and formation conditions remain unknown. Thus, studying hydrothermal springs in this area provides us an opportunity to expand our knowledge of subsurface (bio)chemical processes that generate organic compounds in hydrothermal systems, and aid in future development and exploration of potential energy resources as well. While isotope measurement has long been used for recognition of their origins, there are several secondary processes that may generate variations in isotopic compositions: oxidation, re-equilibration of methane and other alkanes with CO2, mixing with compounds of other sources, etc. Therefore, in addition to isotopic analysis, other evidence, including water chemistry and rock compositions, are necessary to identify volatile compounds of different sources. Surprise Valley Hot Springs (SVHS, 41º32'N, 120º5'W), located in a typical basin and range province valley in northeastern California, is a terrestrial hydrothermal spring system of the Great Basin. Previous geophysical studies indicated the presence of clay-rich volcanic and sedimentary rocks of Tertiary age beneath the lava flows in late Tertiary and Quaternary. Water and gas samples were collected for a variety of chemical and isotope composition analyses, including in-situ pH, alkalinity, conductivity, oxidation reduction potential (ORP), major and trace elements, and C and H isotope measurements. Fluids issuing from SVHS can be classified as Na-(Cl)-SO4 type, with the major cation and anion being Na+ and SO4 2-, respectively. Thermodynamic calculation using ORP and major element data indicated that sulfate is the most dominant sulfur species, which is consistent with anion analysis results. Aquifer temperatures at depth estimated

Modeling patterns of hot water use in households

Energy Technology Data Exchange (ETDEWEB)

Lutz, J.D.; Liu, Xiaomin; McMahon, J.E. [and others

1996-11-01

This report presents a detailed model of hot water use patterns in individual household. The model improves upon an existing model by including the effects of four conditions that were previously unaccounted for: the absence of a clothes washer; the absence of a dishwasher; a household consisting of seniors only; and a household that does not pay for its own hot water use. Although these four conditions can significantly affect residential hot water use, and have been noted in other studies, this is the first time that they have been incorporated into a detailed model. This model allows detailed evaluation of the impact of potential efficiency standards for water heaters and other market transformation policies. 21 refs., 3 figs., 10 tabs.

An assessment of the water quality of the Isinuka springs in the ...

African Journals Online (AJOL)

The physico-chemical properties of Isinuka springs, a "wonder" water resource in Port St Johns area of Eastern Cape Province, were investigated over three seasonal regimes. Water samples were collected from the five spring sources, along Isinuka river and from Ferry Point Cottage spring and analysed for their quality ...

Iron Homeostasis in Yellowstone National Park Hot Spring Microbial Communities

Science.gov (United States)

Brown, I.; Tringe, S. G.; Franklin, H.; Bryant, D. A.; Klatt, C. G.; Sarkisova, S. A.; Guevara, M.

2010-01-01

It has been postulated that life may have originated on Earth, and possibly on Mars, in association with hydrothermal activity and high concentrations of ferrous iron. However, it is not clear how an iron-rich thermal hydrosphere could be hospitable to microbes, since reduced iron appears to stimulate oxidative stress in all domains of life and particularly in oxygenic phototrophs. Therefore, the study of microbial diversity in iron-depositing hot springs (IDHS) and the mechanisms of iron homeostasis and suppression of oxidative stress may help elucidate how Precambrian organisms could withstand the extremely high concentrations of reactive oxygen species (ROS) produced by interaction between environmental Fe(2+) and O2. Proteins and clusters of orthologous groups (COGs) involved in the maintenance of Fe homeostasis found in cyanobacteria (CB) inhabiting environments with high and low [Fe] were main target of this analysis. Preliminary results of the analysis suggest that the Chocolate Pots (CP) microbial community is heavily dominated by phototrophs from the cyanobacteria (CB), Chloroflexi and Chlorobi phyla, while the Mushroom Spring (MS) effluent channel harbors a more diverse community in which Chloroflexi are the dominant phototrophs. It is speculated that CB inhabiting IDHS have an increased tolerance to both high concentrations of Fe(2+) and ROS produced in the Fenton reaction. This hypothesis was explored via a comparative analysis of the diversity of proteins and COGs involved in Fe and redox homeostasis in the CP and MS microbiomes.

Installation package for a solar heating and hot water system

Science.gov (United States)

1978-01-01

Development and installation of two commercial solar heating and hot water systems are reported. The systems consist of the following subsystems: collector, storage, transport, hot water, auxiliary energy and controls. General guidelines are provided which may be utilized in development of detailed installation plans and specifications. In addition, operation, maintenance and repair of a solar heating and hot water system instructions are included.

Isolation and Characterization of Thermophilic Bacteria from Jordanian Hot Springs: Bacillus licheniformis and Thermomonas hydrothermalis Isolates as Potential Producers of Thermostable Enzymes.

Science.gov (United States)

Mohammad, Balsam T; Al Daghistani, Hala I; Jaouani, Atef; Abdel-Latif, Saleh; Kennes, Christian

2017-01-01

The aim of this study was the isolation and characterization of thermophilic bacteria from hot springs in Jordan. Ten isolates were characterized by morphological, microscopic, biochemical, molecular, and physiological characteristics. Sequencing of the 16S rDNA of the isolates followed by BLAST search revealed that nine strains could be identified as Bacillus licheniformis and one isolate as Thermomonas hydrothermalis . This is the first report on the isolation of Thermomonas species from Jordanian hot springs. The isolates showed an ability to produce some thermostable enzymes such as amylase, protease, cellulose, gelatins, and lecithin. Moreover, the UPGMA dendrogram of the enzymatic characteristics of the ten isolates was constructed; results indicated a high phenotypic diversity, which encourages future studies to explore further industrial and environmental applications.

Isolation and Characterization of Thermophilic Bacteria from Jordanian Hot Springs: Bacillus licheniformis and Thermomonas hydrothermalis Isolates as Potential Producers of Thermostable Enzymes

Directory of Open Access Journals (Sweden)

Balsam T. Mohammad

2017-01-01

Full Text Available The aim of this study was the isolation and characterization of thermophilic bacteria from hot springs in Jordan. Ten isolates were characterized by morphological, microscopic, biochemical, molecular, and physiological characteristics. Sequencing of the 16S rDNA of the isolates followed by BLAST search revealed that nine strains could be identified as Bacillus licheniformis and one isolate as Thermomonas hydrothermalis. This is the first report on the isolation of Thermomonas species from Jordanian hot springs. The isolates showed an ability to produce some thermostable enzymes such as amylase, protease, cellulose, gelatins, and lecithin. Moreover, the UPGMA dendrogram of the enzymatic characteristics of the ten isolates was constructed; results indicated a high phenotypic diversity, which encourages future studies to explore further industrial and environmental applications.

Digital data for Quick Response (QR) codes of thermophiles to identify and compare the bacterial species isolated from Unkeshwar hot springs (India)

Science.gov (United States)

Rekadwad, Bhagwan N.; Khobragade, Chandrahasya N.

2015-01-01

16S rRNA sequences of morphologically and biochemically identified 21 thermophilic bacteria isolated from Unkeshwar hot springs (19°85′N and 78°25′E), Dist. Nanded (India) has been deposited in NCBI repository. The 16S rRNA gene sequences were used to generate QR codes for sequences (FASTA format and full Gene Bank information). Diversity among the isolates is compared with known isolates and evaluated using CGR, FCGR and PCA i.e. visual comparison and evaluation respectively. Considerable biodiversity was observed among the identified bacteria isolated from Unkeshwar hot springs. The hyperlinked QR codes, CGR, FCGR and PCA of all the isolates are made available to the users on a portal https://sites.google.com/site/bhagwanrekadwad/. PMID:26793757

Ground-water conditions in Utah, spring of 1994

Science.gov (United States)

Allen, D.V.; Garrett, R.B.; Sory, J.D.; Burden, Carole B.; Danner, M.R.; Herbert, L.R.; Steiger, J.I.; ReMillard, M.D.; Slaugh, B.A.; Swenson, R.L.; Howells, J.H.; Christiansen, H.K.; Bagley, A.D.

1994-01-01

This is the thirty-first in a series of annual reports that describe ground-water conditions in Utah. Reports in this series, published cooperatively by the U.S. Geological Survey and the Utah Division of Water Resources, provide data to enable interested parties to keep abreast of changing ground-water conditions.This report, like the others in the series, contains information on well construction, ground-water withdrawal from wells, water-level changes, related changes in precipitation and streamflow, and chemical quality of water. Supplementary data, such as maps showing water-level contours, are included in reports of this series only for those years or areas for which applicable data are available and are important to a discussion of changing ground-water conditions.This report includes individual discussions of selected significant areas of ground-water development in the State for calendar year 1993. Water-level fluctuations and selected related data, however, are described from the spring of 1989 to the spring of 1994. Much of the data used in this report were collected by the U.S. Geological Survey in cooperation with the Divisions of Water Rights and Water Resources, Utah Department of Natural Resources.

Thioarsenate Formation Coupled with Anaerobic Arsenite Oxidation by a Sulfate-Reducing Bacterium Isolated from a Hot Spring

Directory of Open Access Journals (Sweden)

Geng Wu

2017-07-01

Full Text Available Thioarsenates are common arsenic species in sulfidic geothermal waters, yet little is known about their biogeochemical traits. In the present study, a novel sulfate-reducing bacterial strain Desulfotomaculum TC-1 was isolated from a sulfidic hot spring in Tengchong geothermal area, Yunnan Province, China. The arxA gene, encoding anaerobic arsenite oxidase, was successfully amplified from the genome of strain TC-1, indicating it has a potential ability to oxidize arsenite under anaerobic condition. In anaerobic arsenite oxidation experiments inoculated with strain TC-1, a small amount of arsenate was detected in the beginning but became undetectable over longer time. Thioarsenates (AsO4-xSx2- with x = 1–4 formed with mono-, di- and tri-thioarsenates being dominant forms. Tetrathioarsenate was only detectable at the end of the experiment. These results suggest that thermophilic microbes might be involved in the formation of thioarsenates and provide a possible explanation for the widespread distribution of thioarsenates in terrestrial geothermal environments.

Thioarsenate Formation Coupled with Anaerobic Arsenite Oxidation by a Sulfate-Reducing Bacterium Isolated from a Hot Spring.

Science.gov (United States)

Wu, Geng; Huang, Liuqin; Jiang, Hongchen; Peng, Yue'e; Guo, Wei; Chen, Ziyu; She, Weiyu; Guo, Qinghai; Dong, Hailiang

2017-01-01

Thioarsenates are common arsenic species in sulfidic geothermal waters, yet little is known about their biogeochemical traits. In the present study, a novel sulfate-reducing bacterial strain Desulfotomaculum TC-1 was isolated from a sulfidic hot spring in Tengchong geothermal area, Yunnan Province, China. The arxA gene, encoding anaerobic arsenite oxidase, was successfully amplified from the genome of strain TC-1, indicating it has a potential ability to oxidize arsenite under anaerobic condition. In anaerobic arsenite oxidation experiments inoculated with strain TC-1, a small amount of arsenate was detected in the beginning but became undetectable over longer time. Thioarsenates (AsO 4-x S x 2- with x = 1-4) formed with mono-, di- and tri-thioarsenates being dominant forms. Tetrathioarsenate was only detectable at the end of the experiment. These results suggest that thermophilic microbes might be involved in the formation of thioarsenates and provide a possible explanation for the widespread distribution of thioarsenates in terrestrial geothermal environments.

Hot Water Bathing Impairs Training Adaptation in Elite Teen Archers.

Science.gov (United States)

Hung, Ta-Cheng; Liao, Yi-Hung; Tsai, Yung-Shen; Ferguson-Stegall, Lisa; Kuo, Chia-Hua; Chen, Chung-Yu

2018-04-30

Despite heat imposes considerable physiological stress to human body, hot water immersion remains as a popular relaxation modality for athletes. Here we examined the lingering effect of hot tub relaxation after training on performance-associated measures and dehydroepiandrosterone sulfate (DHEA-S) in junior archers. Ten national level archers, aged 16.6 ± 0.3 years (M = 8, F = 2), participated in a randomized counter-balanced crossover study after baseline measurements. In particular, half participants were assigned to the hot water immersion (HOT) group, whereas another halves were assigned to the untreated control (CON) group. Crossover trial was conducted following a 2-week washout period. During the HOT trial, participants immersed in hot water for 30 min at 40°C, 1 h after training, twice a week (every 3 days) for 2 weeks. Participants during CON trial sat at the same environment without hot water after training. Performance-associated measures and salivary DHEA-S were determined 3 days after the last HOT session. We found that the HOT intervention significantly decreased shooting performance (CON: -4%; HOT: -22%, P HOT: -16%, P HOT: -60%, P < 0.05) of archers, compared with untreated CON trial. No group differences were found in motor unit recruitment (root mean square electromyography, RMS EMG) of arm muscles during aiming, autonomic nervous activity (sympathetic and vagal powers of heart rate variability, HRV), and plasma cortisol levels after treatments. Our data suggest that physiological adaptation against heat exposure takes away the sources needed for normal training adaptation specific to shooting performance in archers.

Geochemistry and hydrothermal alteration at selected Utah hot springs. Final report: Volume 3 (revised)

Energy Technology Data Exchange (ETDEWEB)

Parry, W.T.; Benson, N.L.; Miller, C.D.

1976-07-01

Application of Na-K-Ca geothermometry to warm springs in Utah indicates several areas with sufficiently high apparent temperatures to be of interest as geothermal exploration targets. A zone of warm springs in the Bonneville Basin show Na-K-Ca temperatures from 150/sup 0/C to 233/sup 0/C. Examination of Great Salt Lake, Bonneville sediment pore water, and Jordan Valley well-water chemistry indicates that mixing a small percent of these fluids with warm spring water can cause substantial errors in Na-K-Ca temperature estimates. Other saline deposits which may influence Na-K-Ca temperature estimates are the Paradox formation in southeastern Utah, the Muddy Creek formation in southwestern Utah, the Arapien shale in central Utah, the Preuss formation in northeastern Utah, and Playa salts in much of western Utah. The Roosevelt KGRA is the most attractive target identified by Na-K-Ca geothermometry. Hydrothermal alteration, heavy metal distribution, and water chemistry provide additional characterization of the Roosevelt system. Chemistry of a cool water seep (25/sup 0/C) shows Na-K-Ca temperature of 241/sup 0/C and SiO/sub 2/ temperature of 125/sup 0/C. A Phillips well flowing from below 1500' (457m) shows Na-K-Ca temperature of 262/sup 0/C, SiO/sub 2/ temperature of 262/sup 0/C, and K of 1.5 times the surface spring value. The near surface alteration assemblage is best explained in terms of a decrease in pH of near surface fluids as sulfide oxidizes. Increasing potassium and pH with depth indicates that a K-feldspar stable zone may be intersected with deeper drilling. Geology and alteration were mapped in the Monroe KGRA. (JGB)

Hydrogeochemical characteristics and sources of salinity of the springs near Wenquanzhen in the eastern Sichuan Basin, China

Science.gov (United States)

Guo, Juan; Zhou, Xun; Wang, Lidong; Zhang, Yuqi; Shen, Xiaowei; Zhou, Haiyan; Ye, Shen; Fang, Bin

2017-12-01

Natural springs have the potential to provide important information on hydrogeochemical processes within aquifers. This study used traditional and classic technical methods and procedures to determine the characteristics and evolution of springs to gain further knowledge on the differences between hot saline springs and cold fresh springs. In a short river segment near Wenquanzhen in the eastern Sichuan Basin, southwest China, several natural springs coexist with total dissolved solids (TDS) ranging from less than 1 to 15 g/L and temperatures from 15 to 40 °C. The springs emanate from the outcropping Lower and Middle Triassic carbonates in the river valley cutting the core of an anticline. The cold springs are of Cl·HCO3-Na·Ca and Cl·SO4-Na types, and the hot saline springs are mainly of Cl-Na type. The chemistry of the springs has undergone some changes with time. The stable hydrogen and oxygen isotopes indicate that the spring waters are of a meteoric origin. The salinity of the springs originates from dissolution of minerals, including halite, gypsum, calcite and dolomite. The evolution of the springs involves the following mechanisms: the groundwater receives recharge from infiltration of precipitation, then undergoes deep circulation in the core of the anticline (incongruent dissolution of the salt-bearing strata occurs), and emerges in the river valley in the form of hot springs with high TDS. Groundwater also undergoes shallow circulation in the northern and southern flanks of the anticline and appears in the river valley in the form of cold springs with low TDS.

Geochemical survey for hot and mineral springs, related to the 1997 May 11 Sumikawa landslide, Akita prefecture; 1997 nen 5 gatsu 11 nichi ni hasseishita Akitaken Sumikawa onsen jisuberi ni kanrenshita kinkyu onsen kosen suishitsu chosa

Energy Technology Data Exchange (ETDEWEB)

Takahashi, M.; Endo, H. [Geological Survey of Japan, Tsukuba (Japan)

1997-07-01

Landslide and hydrothermal explosions have occurred near the Sumikawa spa in Akita Prefecture in May 1997. Investigations were performed on correlation of fountains, hot and mineral springs and open cracks seen on aerial photographs taken immediately after the events with the present landslide and hydrothermal explosions. Going toward the Sumikawa spa on the mountaineering path along the Sumikawa river from Onuma can find a flow of valley water, which has not appeared suddenly due to the present landslide (the flow has been existing from long time ago, on which bridges have been installed). The sample water quality has low chloride ion concentration and high sulfuric acid and bicarbonate ion concentrations, and is of high-temperature volcanic gas containing a great amount of hydrogen chloride gas. The water quality was determined non-relative to the present hydrothermal explosions. On the other hand, hot springs and well waters distributed around the Akita Yaeyama and Hachimantai areas have low chloride ion concentration and high sulfuric acid ion concentration. The majority is characterized in that their Ca/Na concentration ratio is relatively low. This suggests that the areas have been formed under common geological conditions. However, the water quality is thought to show different properties due to complex influences of transformation zones and exhalation gas (hydrogen sulfide). 6 refs., 2 figs., 1 tab.

Modeling fluid flow and heat transfer at Basin and Range faults: preliminary results for Leach hot springs, Nevada

Science.gov (United States)

López, Dina L.; Smith, Leslie; Storey, Michael L.; Nielson, Dennis L.

1994-01-01

The hydrothermal systems of the Basin and Range Province are often located at or near major range bounding normal faults. The flow of fluid and energy at these faults is affected by the advective transfer of heat and fluid from an to the adjacent mountain ranges and valleys, This paper addresses the effect of the exchange of fluid and energy between the country rock, the valley fill sediments, and the fault zone, on the fluid and heat flow regimes at the fault plane. For comparative purposes, the conditions simulated are patterned on Leach Hot Springs in southern Grass Valley, Nevada. Our simulations indicated that convection can exist at the fault plane even when the fault is exchanging significant heat and fluid with the surrounding country rock and valley fill sediments. The temperature at the base of the fault decreased with increasing permeability of the country rock. Higher groundwater discharge from the fault and lower temperatures at the base of the fault are favored by high country rock permabilities and fault transmissivities. Preliminary results suggest that basal temperatures and flow rates for Leach Hot Springs can not be simulated with a fault 3 km deep and an average regional heat flow of 150 mW/m2 because the basal temperature and mass discharge rates are too low. A fault permeable to greater depths or a higher regional heat flow may be indicated for these springs.

Radioactive mineral spring precipitates, their analytical and statistical data and the uranium connection

Science.gov (United States)

Cadigan, R.A.; Felmlee, J.K.

1982-01-01

Major radioactive mineral springs are probably related to deep zones of active metamorphism in areas of orogenic tectonism. The most common precipitate is travertine, a chemically precipitated rock composed chiefly of calcium carbonate, but also containing other minerals. The mineral springs are surface manifestations of hydrothermal conduit systems which extend downward many kilometers to hot source rocks. Conduits are kept open by fluid pressure exerted by carbon dioxide-charged waters rising to the surface propelled by heat and gas (CO2 and steam) pressure. On reaching the surface, the dissolved carbon dioxide is released from solution, and calcium carbonate is precipitated. Springs also contain sulfur species (for example, H2S and HS-), and radon, helium and methane as entrained or dissolved gases. The HS- ion can react to form hydrogen sulfide gas, sulfate salts, and native sulfur. Chemical salts and native sulfur precipitate at the surface. The sulfur may partly oxidize to produce detectable sulfur dioxide gas. Radioactivity is due to the presence of radium-226, radon-222, radium-228, and radon-220, and other daughter products of uranium-238 and thorium-232. Uranium and thorium are not present in economically significant amounts in most radioactive spring precipitates. Most radium is coprecipitated at the surface with barite. Barite (barium sulfate) forms in the barium-containing spring water as a product of the oxidation of sulfur species to sulfate ions. The relatively insoluble barium sulfate precipitates and removes much of the radium from solution. Radium coprecipitates to a lesser extent with manganese-barium- and iron-oxy hydroxides. R-mode factor analysis of abundances of elements suggests that 65 percent of the variance of the different elements is affected by seven factors interpreted as follows: (1) Silica and silicate contamination and precipitation; (2) Carbonate travertine precipitation; (3) Radium coprecipitation; (4) Evaporite precipitation

Production and Early Preservation of Lipid Biomarkers in Iron Hot Springs

Energy Technology Data Exchange (ETDEWEB)

Parenteau, Mary N.; Jahnke, Linda L.; Farmer, Jack D.; Cady, Sherry L.

2014-06-01

The bicarbonate-buffered anoxic vent waters at Chocolate Pots hot springs in Yellowstone National Park are 51–54°C, pH 5.5–6.0, and are very high in dissolved Fe(II) at 5.8–5.9 mg/L. The aqueous Fe(II) is oxidized by a combination of biotic and abiotic mechanisms and precipitated as primary siliceous nanophase iron oxyhydroxides (ferrihydrite). Four distinct prokaryotic photosynthetic microbial mat types grow on top of these iron deposits. Lipids were used to characterize the community composition of the microbial mats, link source organisms to geologically significant biomarkers, and investigate how iron mineralization degrades the lipid signature of the community. The phospholipid and glycolipid fatty acid profiles of the highest-temperature mats indicate that they are dominated by cyanobacteria and green nonsulfur filamentous anoxygenic phototrophs (FAPs). Diagnostic lipid biomarkers of the cyanobacteria include midchain branched mono- and dimethylalkanes and, most notably, 2-methylbacteriohopanepolyol. Diagnostic lipid biomarkers of the FAPs (Chloroflexus and Roseiflexus spp.) include wax esters and a long-chain tri-unsaturated alkene. Surprisingly, the lipid biomarkers resisted the earliest stages of microbial degradation and diagenesis to survive in the iron oxides beneath the mats. Understanding the potential of particular sedimentary environments to capture and preserve fossil biosignatures is of vital importance in the selection of the best landing sites for future astrobiological missions to Mars. Finally, this study explores the nature of organic degradation processes in moderately thermal Fe(II)-rich groundwater springs—environmental conditions that have been previously identified as highly relevant for Mars exploration.

Hydrochemical Characteristics and Formation of the Saline or Salty Springs in Eastern Sichuan Basin of China

Science.gov (United States)

Zhou, X.

2017-12-01

Saline or salty springs provide important information on the hydrogeochemical processes and hydrology within subsurface aquifers. More than 20 saline and salty springs occur in the core of anticlines in the eastern Sichuan Basin in southwestern China where the Lower and Middle Triassic carbonates outcrop. Water samples of 8 saline and salty springs (including one saline hot spring) were collected for analyses of the major and minor constituents, trace elements and stable oxygen and hydrogen isotopes. The TDS of the springs range from 4 to 83 g/L, and they are mainly of Cl-Na type. Sr, Ba and Li are the predominant trace elements. The δ2H and δ18O of the water samples indicate that they are of meteoric origin. The source of salinity of the springs originates from dissolution of minerals in the carbonates, including halite, gypsum, calcite and dolomite. The formation mechanism of the springs is that groundwater receives recharge from infiltration of precipitation, undergoes shallow or deep circulation in the core of the anticline and incongruent dissolution of the salt-bearing carbonates occurs, and emerges in the river valley in the form of springs with relatively high TDS. The 8 springs can be classified into 4 springs of shallow groundwater circulation and 4 springs of deep groundwater circulation according to the depth of groundwater circulation, 7 springs of normal temperature and 1 hot spring according to temperature. There are also 2 up-flow springs: the carbonate aquifers are overlain by relatively impervious sandstone and shale, groundwater may flows up to the ground surface through the local portion of the overlying aquiclude where fractures were relatively well developed, and emerges as an up-flow spring. Knowledge of the hydrochemical characteristics and the geneses of the saline and salty springs are of important significance for the utilization and preservation of the springs.

[Isolation and identification of seven thermophilic and anaerobic bacteria from hot springs in Tengchong Rehai].

Science.gov (United States)

Lu, Yueqing; Chen, Bo; Liu, Xiaoli; Ji, Xiuling; Wei, Yunlin; Lin, Lianbing

2009-09-01

In order to study the taxonomic characteristic and physiological, biochemical properties of anaerobic bacteria from hot springs in Tengchong Rehai, Yunnan Province, China. Using Hungate anaerobic technique We isolated seven strains from hot springs in Tengchong Rehai, Yunnan province, and analyzed their 16S rRNA gene sequences. The seven isolates were rod-shaped, Gram-negative, obligate anaerobe, and spores formation was not observed. All strains could grow well at 70 degrees C. Growth of strain RH0802 occurred between 60 and 80 degrees C, optimally around 70 degrees C. The pH range for its growth was between 5.5 and 8.5, with an optimum around 7.0. Strain RH0802 grew on a wide range of carbon sources, including glucose, starch, mannitol, mannose, ribose, maltose, cellobiose, xylose, fructose, galactose, xylan and glycerol, but it could not utilize sucrose or pyruvate. 16S rRNA gene phylogenetic analysis showed that the maximum similarity between the five strains and the strains of genus Caldanaerobacter was up to 98%, except RH0804 and RH0806, which reached to 96% and 93%, respectively. The two isolates were presumed to be potential novel species. The GenBank accession numbers of RH0802 to RH0808 were FJ748766, FJ748762, FJ748761, FJ748763, FJ748765, FJ748764 and FJ748767. The results showed that the seven thermophilic anaerobes belonged to the genus Caldanaerobacter.

Spring cleaning: rural water impacts, valuation, and property rights institutions.

Science.gov (United States)

Kremer, Michael; Leino, Jessica; Miguel, Edward; Zwane, Alix Peterson

2011-01-01

Using a randomized evaluation in Kenya, we measure health impacts of spring protection, an investment that improves source water quality. We also estimate households' valuation of spring protection and simulate the welfare impacts of alternatives to the current system of common property rights in water, which limits incentives for private investment. Spring infrastructure investments reduce fecal contamination by 66%, but household water quality improves less, due to recontamination. Child diarrhea falls by one quarter. Travel-cost based revealed preference estimates of households' valuations are much smaller than both stated preference valuations and health planners' valuations, and are consistent with models in which the demand for health is highly income elastic. We estimate that private property norms would generate little additional investment while imposing large static costs due to above-marginal-cost pricing, private property would function better at higher income levels or under water scarcity, and alternative institutions could yield Pareto improvements.

Measurements of hot water service consumptions: temperature influence

Energy Technology Data Exchange (ETDEWEB)

Secchi, R.; Vallat, D.; Cyssau, R. (COSTIC, Saint Remy-les-Chevreuse (France))

This article presents a campaign of measurements of which the aim is the observation of consumptions, for individual installations equiped with a hot water tank. The study takes an interest in the temperature of the water in the tank and the instantaneous power of the generator. The instrumentation, the installations and the results of this campaign are presented in this paper. The conclusion is the ''economic'' temperature of hot sanitary water is below 60/sup 0/C but above 55/sup 0/C.

Analysis of Spring Development and Gravity Flow System to Capture Water for Local Communities

Directory of Open Access Journals (Sweden)

Adiningrum Cita

2017-01-01

Full Text Available Springs as water sources are relatively inexpensive but highly susceptible to contamination since they are fed by shallow groundwater. Proper spring development helps protect the water from contamination. This study presents an analysis and design of spring development including the type of broncaptering/collecting wall, the dimension for the spring box and the conduction line. In addition, a guideline on “Springwater Construction” published by the Ministry of Public Works has been used in this design. A concentrated spring in Wates, Magelang, Central Java is used as a case study. The design calls for the collection of water from a spring using sets of broncaptering and a spring box, then piping it by gravity a distance of 5.1 kilometers to Van Lith Senior High School. Analysis was done using a manual calculation, which is subsequently compared to the result of HYDROFLO 3 software. Results show that the spring with a flow rate of 0.12 litre/s (manual and 0.17 litre/s (software will be collected into a 5 m3 volume of spring box. The spring box with a +543 m water surface elevation is being supplied to Van Lith +384 m ground elevation using a uniform PVC pipelines with a ¾ inch of diameter.

WATER QUALITY OF THE VRELIĆ SPRING IN DONJE DUBRAVE

Directory of Open Access Journals (Sweden)

Marina Trpčić

2008-12-01

Full Text Available In one part of the groundwater flow in the Vrelić cave (near the village Donje Dubrave, during the explorations in 2003, oil pollution was perceived. During field prospection and contact with local population, few possible pollution sources were located. There was a strong possibility that the oil traces in the cave are the result of the railway accident in 1970. Because of the railway accident on Rijeka-Zagreb railroad, tank carriage sliped off from the tracks and the content of the dangerous cargo leaked onto the nearby valley, 800 meters away from the cave entrance. A spring, used sometimes for water supply by the local population is also located nearby. Sampling of the water from the cave and the spring was carried out several times during the next period with the intention of monitoring the pollution impact on water quality in different seasonal (climatic conditions. In the course of the laboratory analysis of the samples the following parameters were determined: Total hardness; Concentrations of Calcium, Magnesium, Iron, Chlorides and Nitrates; pH-value; TOC; colony-forming unit (CFU; total Coliform; fecal Streptococus; Proteus bacteria; Salmonella bacteria and Clostridium perfringens bacteria. Several of other parameters were also measured by mobile devices: Conductivity (EC, TDS, Redox-potential, pH-value and water temperature. Water tracing with Na-fluorescine was carried out before the analysis and the connection between groundwater flow in cave and the spring water was confirmed. After the creation of a topographical (speleological map of the cave and thanks to the surface (field measurements, the distance between the place of Na-fluorescine spill in the cave and the Vrelić spring was defined (the paper is published in Croatian.

Goodenough Spring, Texas, USA: Discharge and water chemistry of a large spring deeply submerged under the binational Amistad Reservoir

Science.gov (United States)

Kamps, Ray H.; Tatum, Gregg S.; Gault, Mike; Groeger, Alan W.

2009-06-01

Goodenough Spring (Texas, USA) is a large spring near the border of the American state of Texas and the Mexican state of Coahuila, discharging into the international Amistad Reservoir on the river Rio Grande (Rio Bravo). Discharge was routinely measured from 1928 until 1968 to partition the flow of the river between the two countries in accordance with water-use treaties. Samples were analyzed for water-quality parameters in 1967-1968 prior to inundation under 45 m of Amistad Reservoir in 1968. Subsequently, discharge has been estimated indirectly by the International Boundary and Water Commission (IBWC). For the first direct measurements of the spring in 37 years, velocity and cross-sectional measurements were made and water samples collected in the summer of 2005 using advanced self-contained underwater breathing apparatus (SCUBA) techniques. Spring discharge was calculated at 2.03 m3 s-1, approximately one-half of the historical mean of 3.94 m3 s-1. In situ and laboratory analyses of samples for temperature, pH, dissolved oxygen, specific conductance, alkalinity, nitrate-nitrogen, dissolved solids, chloride, sulfate, fluoride, phosphorus, calcium, sodium, potassium, magnesium, and iron showed the water quality to be very good for human consumption and crop irrigation. Measurement values are relatively unchanged from those reported 37 years prior.

Sources and chronology of nitrate contamination in spring waters, Suwannee River basin, Florida

Science.gov (United States)

Katz, Brian G.; Hornsby, H.D.; Bohlke, J.K.; Mokray, M.F.

1999-01-01

A multi-tracer approach, which consisted of analyzing water samples for n aturally occurring chemical and isotopic indicators, was used to better understand sources and chronology of nitrate contamination in spring wate rs discharging to the Suwannee and Santa Fe Rivers in northern Florida. Dur ing 1997 and 1998, as part of a cooperative study between the Suwannee River Water Management District and the U.S. Geological Survey, water samples were collected and analyzed from 24 springs and two wells for major ions, nutrients, dissolved organic carbon, and selected environmental isotopes [18O/16O, D/H, 13C/12C, 15N/14N]. To better understand when nitrate entered the ground-water system, water samples were analyzed for chlorofluorocarbons (CFCs; CCl3F, CCl2F2, and C2Cl3F3) and tritium (3H); in this way, the apparent ages and residence times of spring waters and water from shallow zones in the Upper Floridan aquifer were determined. In addition to information obtained from the use of isotopic and other chemical tracers, information on changes in land-use activities in the basin during 1954-97 were used to estimate nitrogen inputs from nonpoint sources for five counties in the basin. Changes in nitrate concentrations in spring waters with time were compared with estimated nitrogen inputs for Lafayette and Suwannee Counties. Agricultural activities [cropland farming, animal farming operations (beef and dairy cows, poultry, and swine)] along with atmospheric deposition have contributed large quantities of nitrogen to ground water in the Suwannee River Basin in northern Florida. Changes in agricultural land use during the past 40 years in Alachua, Columbia, Gilchrist, Lafayette, and Suwannee Counties have contributed variable amounts of nitrogen to the ground-water system. During 1955-97, total estimated nitrogen from all nonpoint sources (fertilizers, animal wastes, atmospheric deposition, and septic tanks) increased continuously in Gilchrist and Lafayette Counties. In

Minimizing temperature instability of heat recovery hot water system utilizing optimized thermal energy storage

Science.gov (United States)

Suamir, I. N.; Sukadana, I. B. P.; Arsana, M. E.

2018-01-01

One energy-saving technology that starts gaining attractive for hotel industry application in Indonesia is the utilization of waste heat of a central air conditioning system to heat water for domestic hot water supply system. Implementing the technology for such application at a hotel was found that hot water capacity generated from the heat recovery system could satisfy domestic hot water demand of the hotel. The gas boilers installed in order to back up the system have never been used. The hot water supply, however, was found to be instable with hot water supply temperature fluctuated ranging from 45 °C to 62 °C. The temperature fluctuations reaches 17 °C, which is considered instable and can reduce hot water usage comfort level. This research is aimed to optimize the thermal energy storage in order to minimize the temperature instability of heat recovery hot water supply system. The research is a case study approach based on cooling and hot water demands of a hotel in Jakarta-Indonesia that has applied water cooled chillers with heat recovery systems. The hotel operation with 329 guest rooms and 8 function rooms showed that hot water production in the heat recovery system completed with 5 m3 thermal energy storage (TES) could not hold the hot water supply temperature constantly. The variations of the cooling demand and hot water demands day by day were identified. It was found that there was significant mismatched of available time (hours) between cooling demand which is directly correlated to the hot water production from the heat recovery system and hot water usage. The available TES system could not store heat rejected from the condenser of the chiller during cooling demand peak time between 14.00 and 18.00 hours. The extra heat from the heat recovery system consequently increases the temperature of hot water up to 62 °C. It is about 12 K above 50 °C the requirement hot water temperature of the hotel. In contrast, the TES could not deliver proper

Recovery of energy from geothermal brine and other hot water sources

Science.gov (United States)

Wahl, III, Edward F.; Boucher, Frederic B.

1981-01-01

Process and system for recovery of energy from geothermal brines and other hot water sources, by direct contact heat exchange between the brine or hot water, and an immiscible working fluid, e.g. a hydrocarbon such as isobutane, in a heat exchange column, the brine or hot water therein flowing countercurrent to the flow of the working fluid. The column can be operated at subcritical, critical or above the critical pressure of the working fluid. Preferably, the column is provided with a plurality of sieve plates, and the heat exchange process and column, e.g. with respect to the design of such plates, number of plates employed, spacing between plates, area thereof, column diameter, and the like, are designed to achieve maximum throughput of brine or hot water and reduction in temperature differential at the respective stages or plates between the brine or hot water and the working fluid, and so minimize lost work and maximize efficiency, and minimize scale deposition from hot water containing fluid including salts, such as brine. Maximum throughput approximates minimum cost of electricity which can be produced by conversion of the recovered thermal energy to electrical energy.

Natural radioactivity of bedrock bath instruments and hot spring instruments in Japan

International Nuclear Information System (INIS)

Kazuki Iwaoka; Hiroyuki Tabe; Hidenori Yonehara

2013-01-01

In Japan, bedrock bath instruments and hot spring instruments that contain natural radioactive nuclides are commercially available. In this study, such instruments containing natural radioactive nuclides, currently distributed in Japan, were collected and the radioactivity concentration of 238 U series, 232 Th series, and 40 K in them was determined by gamma ray spectrum analyses. Effective doses to workers and general consumers handling the materials were estimated, revealing the radioactivity concentration of 238 U series, 232 Th series, and 40 K to be lower than critical values given in the IAEA Safety Guide. The maximum effective doses to workers and general consumers were 210 and 6.1 μSv y -1 , respectively. These values are lower than the intervention exemption level (1,000 μSv y -1 ) given in ICRP Publ. 82. (author)

Microbiological and chemical assessment of spring water from a ...

African Journals Online (AJOL)

Assessment of spring water from Ikare-Akoko, a rural setting in southwest, Nigeria for microbial and chemical contaminants was carried out. Total heterotrophic bacteria count of 4.0 x 106 CFU/mL was highest during the peak of the rainy season in Omi-idu spring (Igbede community) while the lowest population of 0.14 x106 ...

Saberes e práticas termais: uma perspectiva comparada em Portugal (Termas de S. Pedro do Sul e no Brasil (Caldas da Imperatriz Thermal knowledge and therapies: a comparative view of Portugal (São Pedro do Sul hot springs and Brazil (Caldas da Imperatriz hot springs

Directory of Open Access Journals (Sweden)

Maria Manuel Quintela

2004-01-01

Full Text Available Pretende-se neste artigo discutir como o termalismo, como prática terapêutica, se constituiu como um saber dito 'científico' legitimado pela medicina, que criou estabelecimentos terapêuticos e estâncias termais. Tomaremos como fontes textos dos séculos XIX e XX. A pesquisa foi iniciada em Portugal, em 1996, sendo efetuada uma etnografia das experiências termais nas Termas de S. Pedro do Sul. No Brasil, a pesquisa nas Caldas da Imperatriz foi iniciada em agosto de 2001 e encontra-se ainda em curso.Based on nineteenth- and twentieth-century texts, the article discusses how medicine legitimized the therapeutic practice of thermalism as so-called 'scientific' knowledge, with the creation of therapeutic establishments and hot-springs resorts. My research began in Portugal in 1996, where I produced an ethnography of experiences at the São Pedro do Sul hot springs. My research at Brazil's Caldas da Imperatriz, initiated in August 2001, is still underway.

Preliminary design package for solar heating and hot water system

Science.gov (United States)

1976-01-01

Two prototype solar heating and hot water systems for use in single-family dwellings or commercial buildings were designed. Subsystems included are: collector, storage, transport, hot water, auxiliary energy, and government-furnished site data acquisition. The systems are designed for Yosemite, California, and Pueblo, Colorado. The necessary information to evaluate the preliminary design for these solar heating and hot water systems is presented. Included are a proposed instrumentation plan, a training program, hazard analysis, preliminary design drawings, and other information about the design of the system.

Screening of Thermophilic Bacteria Produce Xylanase from Sapan Sungai Aro Hot Spring South Solok

Science.gov (United States)

Irdawati, I.; Syamsuardi, S.; Agustien, A.; Rilda, Y.

2018-04-01

xylanase is one of the enzymes with great prospects as hemicellulose hydrolyzing enzyme. Global annual market demand for this enzyme reach US 200 million. This enzyme catalyzes the xylan (hemicellulose) reactions breaking into xilooligosakarida and xylose. Xylanase can be applied to various industrial sectors such as bread, sugar xylose, biofuels, especially in bleaching paper (bleaching) pulp. Xylanase Isable to replace conventional chemical bleaching using chlorine that is not friendly for the environment. Currently xylanase production is extracted from the thermophilic bacteria for enzyme stability at high temperatures that are suitable for industrial applications. Thermophilic bacteria can be isolated from a hot spring, one of the which is a source of Sapan Sungai Aro Hot Spring, located in the district South Solok. The aim of this study was to select and identification of thermophilic bacteria can produce xylanase.This roomates is a descriptive study, which was Carried out in the Laboratory of Microbiology, Mathematic and Science Faculty of Padang State University, and Laboratory of Bacteriology, BasoVeterinary Research Center. The research procedure consisted of the preparation and sterilization of materials and tools, medium manufacturing, regeneration, selection and identification. Selection is performed by using a semiquantitative screening plate that contains xylan substrate. Identification is based on microscopic and biochemical characteristics until the genus level.Selection results Showed 12 out of 16 isolates had xilanolitik activity, with the highest activity is SSA2 with xilanolitik index of 0.74. The top five index producehigestxilanolitik isolates that are SSA2, SSA3 and SSA4 identified as Bacillus sp. 1., and SSAS6 and SSA7 is Bacillus sp. 2.

Nitrate concentration in spring water at the Nogawa basin and its possible source

International Nuclear Information System (INIS)

Yoshida, Kazuhiro; Ogura, Norio

1978-01-01

Fluctuation of nitrate concentration in spring water at the Nogawa basin was studied during 1976 - 1977, and the possible source of nitrate nitrogen was discussed. Nitrate concentration in spring water at the station N-O in Kokubunji, Tokyo ranged from 360 to 574 μg at/l with an average value of 502 μg at/l. It seemed that the effluent of spring water at N-O was influenced by rainfall within a short period. A laboratory experiment on production of nitrate in soil showed that ammonium nitrogen added to fresh soil was transformed quantitatively to nitrate nitrogen during 23 days incubation. Thd sup(delta15)N value of nitrate nitrogen in spring water (+0.89%) was similar to that of ammonium nitrogen in sewage (+0.82%) discharging into the Nogawa River. In the area near N-O, domestic wastes have been discharged into the Nogawa River by simple sewers or percolated downward through the soil. These results suggest that one of the main source of nitrate nitrogen in spring water is ammonium and organic nitrogen in domestic wastes. (author)

Motel solar-hot-water system with nonpressurized storage--Jacksonville, Florida

Science.gov (United States)

1981-01-01

Modular roof-mounted copper-plated arrays collect solar energy; heated water drains from them into 1,000 gallon nonpressurized storage tank which supplies energy to existing pressurized motel hot water lines. System provides 65 percent of hot water demand. Report described systems parts and operation, maintenance, and performance and provides warranty information.

Subaqueous hot springs in Köyceğiz Lake, Dalyan Channel and Fethiye-Göcek Bay (SW Turkey): Locations, chemistry and origins

KAUST Repository

Avşar, Ö zgü r; Avsar, Ulas; Arslan, Şebnem; Kurtuluş, Bedri; Niedermann, Samuel; Gü leç , Nilgü n

2017-01-01

In this study, horizontal temperature measurements along organized grids have been used to detect subaqueous hot springs. The study area, located in the southwest of Turkey and comprised of Köyceğiz Lake, Dalyan Channel and Fethiye-Göcek Bay

Sources of nitrate contamination and age of water in large karstic springs of Florida

Science.gov (United States)

Katz, B.G.

2004-01-01

In response to concerns about the steady increase in nitrate concentrations over the past several decades in many of Florida's first magnitude spring waters (discharge ???2.8 m3/s), multiple isotopic and other chemical tracers were analyzed in water samples from 12 large springs to assess sources and timescales of nitrate contamination. Nitrate-N concentrations in spring waters ranged from 0.50 to 4.2 mg/L, and ??15N values of nitrate in spring waters ranged from 2.6 to 7.9 per mil. Most ??15N values were below 6 per mil indicating that inorganic fertilizers were the dominant source of nitrogen in these waters. Apparent ages of groundwater discharging from springs ranged from 5 to about 35 years, based on multi-tracer analyses (CFC-12, CFC-113, SF6, 3H/3He) and a piston flow assumption; however, apparent tracer ages generally were not concordant. The most reliable spring-water ages appear to be based on tritium and 3He data, because concentrations of CFCs and SF6 in several spring waters were much higher than would be expected from equilibration with modern atmospheric concentrations. Data for all tracers were most consistent with output curves for exponential and binary mixing models that represent mixtures of water in the Upper Floridan aquifer recharged since the early 1960s. Given that groundwater transit times are on the order of decades and are related to the prolonged input of nitrogen from multiple sources to the aquifer, nitrate could persist in groundwater that flows toward springs for several decades due to slow transport of solutes through the aquifer matrix.

Characterization of Thermophilic Halotolerant Aeribacillus pallidus TD1 from Tao Dam Hot Spring, Thailand

OpenAIRE

Yasawong, Montri; Areekit, Supatra; Pakpitchareon, Arda; Santiwatanakul, Somchai; Chansiri, Kosum

2011-01-01

The bacterial strain TD1 was isolated from Tao Dam hot spring in Thailand. Strain TD1 was Gram positive, rod-shaped, aerobic, motile, and endospore forming. The cell was 2.0–40 mm in length and about 0.4 mm in diameter. The optimum growth occurred at 55–60 °C and at pH 7–8. Strain TD1 was able to grow on medium containing up to 10% NaCl. The DNA G+C content was 38.9 mol%. The cellular fatty acid content was mainly C16:0, which comprised 25.04% of the total amount of cellular fatty acid. 16S r...

Characterization of novel bacteriochlorophyll-a-containing red filaments from alkaline hot springs in Yellowstone National Park.

Science.gov (United States)

Boomer, S M; Pierson, B K; Austinhirst, R; Castenholz, R W

2000-09-01

Novel red, filamentous, gliding bacteria formed deep red layers in several alkaline hot springs in Yellowstone National Park. Filaments contained densely layered intracellular membranes and bacteriochlorophyll a. The in vivo absorption spectrum of the red layer filaments was distinct from other phototrophs, with unusual bacteriochlorophyll a signature peaks in the near-infrared (IR) region (807 nm and 911 nm). These absorption peaks were similar to the wavelengths penetrating to the red layer of the mats as measured with in situ spectroradiometry. The filaments also demonstrated maximal photosynthetic uptake of radiolabeled carbon sources at these wavelengths. The red layer filaments displayed anoxygenic photoheterotrophy, as evidenced by the specific incorporation of acetate, not bicarbonate, and by the absence of oxygen production. Photoheterotrophy was unaffected by sulfide and oxygen, but was diminished by high-intensity visible light. Near-IR radiation supported photoheterotrophy. Morphologically and spectrally similar filaments were observed in several springs in Yellowstone National Park, including Octopus Spring. Taken together, these data suggest that the red layer filaments are most similar to the photoheterotroph, Heliothrix oregonensis. Notable differences include mat position and coloration, absorption spectra, and prominent intracellular membranes.

Validation of a Hot Water Distribution Model Using Laboratory and Field Data

Energy Technology Data Exchange (ETDEWEB)

Backman, C.; Hoeschele, M.

2013-07-01

Characterizing the performance of hot water distribution systems is a critical step in developing best practice guidelines for the design and installation of high performance hot water systems. Developing and validating simulation models is critical to this effort, as well as collecting accurate input data to drive the models. In this project, the ARBI team validated the newly developed TRNSYS Type 604 pipe model against both detailed laboratory and field distribution system performance data. Validation efforts indicate that the model performs very well in handling different pipe materials, insulation cases, and varying hot water load conditions. Limitations of the model include the complexity of setting up the input file and long simulation run times. In addition to completing validation activities, this project looked at recent field hot water studies to better understand use patterns and potential behavioral changes as homeowners convert from conventional storage water heaters to gas tankless units. Based on these datasets, we conclude that the current Energy Factor test procedure overestimates typical use and underestimates the number of hot water draws. This has implications for both equipment and distribution system performance. Gas tankless water heaters were found to impact how people use hot water, but the data does not necessarily suggest an increase in usage. Further study in hot water usage and patterns is needed to better define these characteristics in different climates and home vintages.

Validation of a Hot Water Distribution Model Using Laboratory and Field Data

Energy Technology Data Exchange (ETDEWEB)

Backman, C. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Hoeschele, M. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States)

2013-07-01

Characterizing the performance of hot water distribution systems is a critical step in developing best practice guidelines for the design and installation of high performance hot water systems. Developing and validating simulation models is critical to this effort, as well as collecting accurate input data to drive the models. In this project, the Building America research team ARBI validated the newly developed TRNSYS Type 604 pipe model against both detailed laboratory and field distribution system performance data. Validation efforts indicate that the model performs very well in handling different pipe materials, insulation cases, and varying hot water load conditions. Limitations of the model include the complexity of setting up the input file and long simulation run times. This project also looked at recent field hot water studies to better understand use patterns and potential behavioral changes as homeowners convert from conventional storage water heaters to gas tankless units. The team concluded that the current Energy Factor test procedure overestimates typical use and underestimates the number of hot water draws, which has implications for both equipment and distribution system performance. Gas tankless water heaters were found to impact how people use hot water, but the data does not necessarily suggest an increase in usage. Further study in hot water usage and patterns is needed to better define these characteristics in different climates and home vintages.

Origin and geochemistry of saline spring waters in the Athabasca oil sands region, Alberta, Canada

International Nuclear Information System (INIS)

Gue, Anita E.; Mayer, Bernhard; Grasby, Stephen E.

2015-01-01

Highlights: • Saline groundwater enters the Athabasca and Clearwater rivers in the AOSR via springs. • High TDS is due to subsurface dissolution of Devonian evaporites and carbonates. • Low δ 18 O values, and 3 H and 14 C data suggest some Laurentide glacial meltwater input. • Bacterial sulfate reduction, methanogenesis, and CH 4 oxidation were identified. • Metal and PAH contents are reported; bitumen does not appear to be major influence. - Abstract: The geochemistry of saline spring waters in the Athabasca oil sands region (AOSR) in Alberta (Canada) discharging from Devonian carbonate rocks into the Athabasca and Clearwater rivers was characterized for major ions, trace elements, dissolved gases, and polycyclic aromatic hydrocarbons (PAHs). In addition, stable isotope analyses of H 2 O, SO 4 , dissolved inorganic carbon (DIC), Sr, and CH 4 were used to trace the sources of spring waters and their dissolved solutes, and to identify subsurface processes affecting water chemistry. The spring waters had δ 18 O values as low as −23.5‰, suggesting they are composed of up to 75% Laurentide glacial meltwater. Tritium and radiocarbon age-dating results, analyzed for three spring waters, supported a glacial origin. The high salinity of the spring waters (TDS 7210–51,800 mg/L) was due to dissolution of Devonian evaporite and carbonate deposits in the subsurface. Spring waters were affected by bacterial (dissimilatory) sulfate reduction, methanogenesis, and methane oxidation. Trace elements were present in spring waters at varying concentrations, with only one spring containing several predominant oil sands metals (As, Fe, Mo, Ni, Se, Zn) suggesting bitumen as a source. Five springs contained elements (Al, As, B, Fe, Se) at concentrations exceeding water quality guidelines for the protection of aquatic life. Seven PAHs were detected in spring waters (total PAH concentrations ranged from 7.3 to 273.6 ng/L), but most springs contained a maximum of two PAHs

Ikaite precipitation by mixing of shoreline springs and lake water, Mono Lake, California, USA

Science.gov (United States)

Bischoff, James L.; Stine, Scott; Rosenbauer, Robert J.; Fitzpatrick, John A.; Stafford, Thomas W., Jr.

1993-08-01

Metastable ikaite (CaCO 3·6H 2O) forms abundantly during winter months along the south shoreline of Mono Lake where shoreline springs mix with lake water. Ikaite precipitates because of its decreased solubility at low temperature and because of orthophosphate-ion inhibition of calcite and aragonite. During the spring some of the ikaite is transformed to anhydrous CaCO 3 and is incorporated into tufa, but most is dispersed by wave action into the lake where it reacts to form gaylussite (Na 2Ca(CO 3) 2· 5H 2O). Spring waters have low pH values, are dominantly Ca-Na-HCO 3, have low radiocarbon activities, and are mixtures of deep-seated geothermal and cold groundwaters. Chemical modeling reveals that precipitation of CaCO 3 can occur over a broad range of mixtures of spring and lake water with a maximum production occurring at 96% spring water and 4% lake water. Under these conditions all the Ca and a significant fraction of the CO 3 of the precipitate is spring supplied. A radiocarbon age of 19,580 years obtained on a natural ikaite sample supports this conclusion. With the springs supplying a large and probably variable portion of the carbonate, and with apparent 14C age of the carbonate varying from spring to spring, tufa of similar actual antiquity may yield significantly different 14C dates, making tufa at this location unsuitable for absolute age dating by the radiocarbon method.

Time-integrated radon measurements in spring and well waters by track technique

International Nuclear Information System (INIS)

Somogyi, G.

1986-01-01

The radon content dissolved in natural waters seems to be a very sensitive indicator of potential uranium deposits. We have developed different track methods to perform time-integrated, ''in-situ'' measurements of radon in different natural waters (spring, lake, well) and their neighbouring soil gas. One of our main purposes was to study the seasonal variation of radon content and its possible correlation with certain water (yield, flow rate) and environmental (depth, temperature) parameters. Simultaneous radon measurements have been carried out in lake and spring waters in a cave, in thermal and cold water springs of a public bath and in a deep drilled well. The radon profiles obtained in the deep well lend support to the idea that the environmental radon can travel large distances in microbubbles of a ''carrier geogas''. (author)

Time scale of hydrothermal water-rock reactions in Yellowstone National Park based on radium isotopes and radon

International Nuclear Information System (INIS)

Clark, J.F.; Turekian, K.K.

1990-01-01

We have measured 224 Ra (3.4 d), 228 Ra (5.7 yr), and 226 Ra (1620 yr) and chloride in hot spring waters from the Norris-Mammoth Corridor, Yellowstone National Park. Two characteristic cold-water components mix with the primary hydrothermal water: one for the travertine-depositing water related to the Mammoth Hot Springs and the other for the sinter-depositing Norris Geyser Basin springs. The Mammoth Hot Springs water is a mixture of the primary hydrothermal fluid with meteoric waters flowing through the Madison Limestone, as shown by the systematic decrease of the ( 228 Ra/ 226 Ra) activity ratio proceeding northward. The Norris Geyser Basin springs are mixtures of primary hydrothermal water with different amounts of cold meteoric water with no modification of the primary hydrothermal ( 228 Ra/ 226 Ra) activity ratio. Using a solution and recoil model for radium isotope supply to the primary hydrothermal water, a mean water-rock reaction time prior to expansion at 350degC and supply to the surface is 540 years assuming that 250 g of water are involved in the release of the radium from one gram of rock. The maximum reaction time allowed by our model is 1150 years. (orig.)

Design data brochure: Solar hot water system

Science.gov (United States)

1978-01-01

A design calculation is detailed for a single-family residence housing a family of four in a nonspecific geographical area. The solar water heater system is designed to provide 80 gallons of 140 F hot water per day.

Surprisingly low natural gas consumption for hot water in the Netherlands in 1996

International Nuclear Information System (INIS)

Geerse, C.

1997-01-01

The Dutch use hot water more efficient than previously expected. This conclusion is drawn from a recent study of hot water consumption in Dutch households and the corresponding natural gas consumption. Based on that (once-only) hot water use survey the hot water use models, as applied in the annual Basic Survey of Natural Gas Consumption of Small-scale Consumers in the Netherlands (BAK), will be modified. 6 tabs

Prototype solar heating and cooling systems including potable hot water

Science.gov (United States)

1978-01-01

Progress is reviewed in the development, delivery, and support of two prototype solar heating and cooling systems including potable hot water. The system consisted of the following subsystems: collector, auxiliary heating, potable hot water, storage, control, transport, and government-furnished site data acquisition.

Forecasting HotWater Consumption in Residential Houses

Directory of Open Access Journals (Sweden)

Linas Gelažanskas

2015-11-01

Full Text Available An increased number of intermittent renewables poses a threat to the system balance. As a result, new tools and concepts, like advanced demand-side management and smart grid technologies, are required for the demand to meet supply. There is a need for higher consumer awareness and automatic response to a shortage or surplus of electricity. The distributed water heater can be considered as one of the most energy-intensive devices, where its energy demand is shiftable in time without influencing the comfort level. Tailored hot water usage predictions and advanced control techniques could enable these devices to supply ancillary energy balancing services. The paper analyses a set of hot water consumption data from residential dwellings. This work is an important foundation for the development of a demand-side management strategy based on hot water consumption forecasting at the level of individual residential houses. Various forecasting models, such as exponential smoothing, seasonal autoregressive integrated moving average, seasonal decomposition and a combination of them, are fitted to test different prediction techniques. These models outperform the chosen benchmark models (mean, naive and seasonal naive and show better performance measure values. The results suggest that seasonal decomposition of the time series plays the most significant part in the accuracy of forecasting.

Solar action: solar hot water in The Netherlands

International Nuclear Information System (INIS)

Van de Water, Adrie

2001-01-01

This paper focuses on the use of solar hot water systems in the Netherlands, and reports on the Dutch Solar Domestic Hot Water System agreement signed in 1999 and set up to enhance the development of the market for solar domestic hot water (SDHW) systems and their application as a sustainable energy source. The Dutch Thermal Solar Energy Programme's objectives and goals, the subsidy schemes for thermal solar energy administered by Senter - an agency of the Ministry of Economic Affairs (MEA), and the project-based and individual approaches to boosting the sales of SDHW systems are examined. Large system sales, the targeting of consumers via a national campaign, and national publicity using the slogan 'Sustainable energy. Goes without saying' commissioned by the MEA are discussed along with the support shown by the Dutch power distribution companies for SDHW systems, marketing aspects, and the outlook for sales of SDHW systems

Timescales for nitrate contamination of spring waters, northern Florida, USA

Science.gov (United States)

Katz, B.G.; Böhlke, J.K.; Hornsby, H.D.

2001-01-01

Residence times of groundwater, discharging from springs in the middle Suwannee River Basin, were estimated using chlorofluorocarbons (CFCs), tritium (3H), and tritium/helium-3 (3H/3He) age-dating methods to assess the chronology of nitrate contamination of spring waters in northern Florida. During base-flow conditions for the Suwannee River in 1997–1999, 17 water samples were collected from 12 first, second, and third magnitude springs discharging groundwater from the Upper Floridan aquifer. Extending age-dating techniques, using transient tracers to spring waters in complex karst systems, required an assessment of several models [piston-flow (PFM), exponential mixing (EMM), and binary-mixing (BMM)] to account for different distributions of groundwater age. Multi-tracer analyses of four springs yielded generally concordant PFM ages of around 20±2 years from CFC-12, CFC-113, 3H, and 3He, with evidence of partial CFC-11 degradation. The EMM gave a reasonable fit to CFC-113, CFC-12, and 3H data, but did not reproduce the observed 3He concentrations or 3H/3He ratios, nor did a combination PFM–EMM. The BMM could reproduce most of the multi-tracer data set only if both endmembers had 3H concentrations not much different from modern values. CFC analyses of 14 additional springs yielded apparent PFM ages from about 10 to 20 years from CFC-113, with evidence of partial CFC-11 degradation and variable CFC-12 contamination. While it is not conclusive, with respect to the age distribution within each spring, the data indicate that the average residence times were in the order of 10–20 years and were roughly proportional to spring magnitude. Applying similar models to recharge and discharge of nitrate based on historical nitrogen loading data yielded contrasting trends for Suwanee County and Lafayette County. In Suwanee County, spring nitrate trends and nitrogen isotope data were consistent with a peak in fertilizer input in the 1970s and a relatively high overall ratio

National uranium resource evaluation, Hot Springs Quadrangle, South Dakota and Nebraska

International Nuclear Information System (INIS)

Truesdell, D.B.; Daddazio, P.L.; Martin, T.S.

1982-06-01

The Hot Springs Quadrangle, South Dakota and Nebraska, was evaluated to a depth of 1500 m to identify environments and delineate areas favorable for the occurrence of uranium deposits. The evaluation used criteria developed by the National Uranium Resource Evaluation program. Surface reconnaissance was conducted using a portable scintillometer and a gamma spectrometer. Geochemical sampling was carried out in all geologic environments accessible within the quadrangle. Additional investigations included the followup of aerial radiometric and hydrogeochemical anomalies and a subsurface study. Environments favorable for sandstone-type deposits occur in the Inyan Kara Group and Chadron Member of the White River Group. Environments favorable for marine black-shale deposits occur in the Hayden Member of the Minnelusa Formation. A small area of the Harney Peak Granite is favorable for authigenic deposits. Environments considered unfavorable for uranium deposits are the Precambrian granitic and metasedimentary rocks and Paleozoic, Mesozoic, and Tertiary sedimentary rocks other than those previously mentioned

Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils.

Science.gov (United States)

Aanniz, Tarik; Ouadghiri, Mouna; Melloul, Marouane; Swings, Jean; Elfahime, Elmostafa; Ibijbijen, Jamal; Ismaili, Mohamed; Amar, Mohamed

2015-06-01

The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.

Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils

Directory of Open Access Journals (Sweden)

Tarik Aanniz

2015-06-01

Full Text Available The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240 thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5% represented by B. licheniformis (119, B. aerius (44, B. sonorensis (33, B. subtilis (subsp. spizizenii (2 and subsp. inaquosurum (6, B. amyloliquefaciens (subsp. amyloliquefaciens (4 and subsp. plantarum (4, B. tequilensis (3, B. pumilus (3 and Bacillus sp. (19. Only six isolates (2.5% belonged to the genus Aeribacillus represented by A. pallidus (4 and Aeribacillus sp. (2. In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.

Time-integrated radon measurements in spring and well waters by track technique

Energy Technology Data Exchange (ETDEWEB)

Somogyi, G.; Lenart, L.

1986-01-01

The radon content dissolved in natural waters seems to be a very sensitive indicator of potential uranium deposits. We have developed different track methods to perform time-integrated, ''in-situ'' measurements of radon in different natural waters (spring, lake, well) and their neighbouring soil gas. One of our main purposes was to study the seasonal variation of radon content and its possible correlation with certain water (yield, flow rate) and environmental (depth, temperature) parameters. Simultaneous radon measurements have been carried out in lake and spring waters in a cave, in thermal and cold water springs of a public bath and in a deep drilled well. The radon profiles obtained in the deep well lend support to the idea that the environmental radon can travel large distances in microbubbles of a ''carrier geogas''.

Organic compounds in hot-water-soluble fractions from water repellent soils

Science.gov (United States)

Atanassova, Irena; Doerr, Stefan

2014-05-01

Water repellency (WR) is a soil property providing hydrophobic protection and preventing rapid microbial decomposition of organic matter entering the soil with litter or plant residues. Global warming can cause changes in WR, thus influencing water storage and plant productivity. Here we assess two different approaches for analysis of organic compounds composition in hot water extracts from accelerated solvent extraction (ASE) of water repellent soils. Extracts were lyophilized, fractionated on SiO2 (sand) and SPE cartridge, and measured by GC/MS. Dominant compounds were aromatic acids, short chain dicarboxylic acids (C4-C9), sugars, short chain fatty acids (C8-C18), and esters of stearic and palmitic acids. Polar compounds (mainly sugars) were adsorbed on applying SPE clean-up procedure, while esters were highly abundant. In addition to the removal of polar compounds, hydrophobic esters and hydrocarbons (alkanes and alkenes particle wettability and C dynamics in soils. Key words: soil water repellency, hot water soluble carbon (HWSC), GC/MS, hydrophobic compounds

Phylogenetic Evidence for the Existence of Novel Thermophilic Bacteria in Hot Spring Sulfur-Turf Microbial Mats in Japan

Science.gov (United States)

Yamamoto, Hiroyuki; Hiraishi, Akira; Kato, Kenji; Chiura, Hiroshi X.; Maki, Yonosuke; Shimizu, Akira

1998-01-01

So-called sulfur-turf microbial mats, which are macroscopic white filaments or bundles consisting of large sausage-shaped bacteria and elemental sulfur particles, occur in sulfide-containing hot springs in Japan. However, no thermophiles from sulfur-turf mats have yet been isolated as cultivable strains. This study was undertaken to determine the phylogenetic positions of the sausage-shaped bacteria in sulfur-turf mats by direct cloning and sequencing of 16S rRNA genes amplified from the bulk DNAs of the mats. Common clones with 16S rDNA sequences with similarity levels of 94.8 to 99% were isolated from sulfur-turf mat samples from two geographically remote hot springs. Phylogenetic analysis showed that the phylotypes of the common clones formed a major cluster with members of the Aquifex-Hydrogenobacter complex, which represents the most deeply branching lineage of the domain bacteria. Furthermore, the bacteria of the sulfur-turf mat phylotypes formed a clade distinguishable from that of other members of the Aquifex-Hydrogenobacter complex at the order or subclass level. In situ hybridization with clone-specific probes for 16S rRNA revealed that the common phylotype of sulfur-turf mat bacteria is that of the predominant sausage-shaped bacteria. PMID:9572936

Emergency cooling system with hot-water jet pumps for nuclear reactors

International Nuclear Information System (INIS)

Reinsch, A.O.W.

1977-01-01

The ECCS for a PWR or BWR uses hot-water jet pumps to remove the thermal energy generated in the reactor vessel and stored in the water. The hot water expands in the nozzle part (Laval nozzle) of the jet pump and sucks in coolant (borated water) coming from a storage tank containing subcooled water. This water is mixing with the hot water/steam mixture from the Laval nozzle. The steam is condensed. The kinetic energy of the water is converted into a pressure increase which is sufficient to feed the water into the reactor vessel. The emergency cooling may further be helped by a jet condenser also operating according to the principle of a jet pump and condensing the steam generated in the reactor vessel. (DG) [de

Toward Complete Utilization of Miscanthus in a Hot-Water Extraction-Based Biorefinery

Directory of Open Access Journals (Sweden)

Kuo-Ting Wang

2017-12-01

Full Text Available Miscanthus (Miscanthus sp. Family: Poaceae was hot-water extracted (two h, at 160 °C at three scales: laboratory (Parr reactor, 300 cm3, intermediate (M/K digester, 4000 cm3, and pilot (65 ft3-digester, 1.841 × 106 cm3. Hot-water extracted miscanthus, hydrolyzate, and lignin recovered from hydrolyzate were characterized and evaluated for potential uses aiming at complete utilization of miscanthus. Effects of scale-up on digester yield, removal of hemicelluloses, deashing, delignification degree, lignin recovery and purity, and cellulose retention were studied. The scale-dependent results demonstrated that before implementation, hot-water extraction (HWE should be evaluated on a scale larger than a laboratory scale. The production of energy-enriched fuel pellets from hot-water extracted miscanthus, especially in combination with recovered lignin is recommended, as energy of combustion increased gradually from native to hot-water extracted miscanthus to recovered lignin. The native and pilot-scale hot-water extracted miscanthus samples were also subjected to enzymatic hydrolysis using a cellulase-hemicellulase cocktail, to produce fermentable sugars. Hot-water extracted biomass released higher amount of glucose and xylose verifying benefits of HWE as an effective pretreatment for xylan-rich lignocellulosics. The recovered lignin was used to prepare a formaldehyde-free alternative to phenol-formaldehyde resins and as an antioxidant. Promising results were obtained for these lignin valorization pathways.

Apparent CFC and 3H/ 3He age differences in water from Floridan Aquifer springs

Science.gov (United States)

Happell, James D.; Opsahl, Stephen; Top, Zafer; Chanton, Jeffrey P.

2006-03-01

The apparent CFC-11, -12 and -113 ages of Upper Floridan Aquifer water discharged from 31 springs located in Florida and Georgia ranged from 11 to 44 years when samples were collected in 2002 and 2003. Apparent 3H/ 3He ages in these springs ranged from 12 to 66 years. Some of the springs sampled did not yield valid CFC ages because one or more of the CFCs were contaminated by non-atmospheric sources. Of the 31 springs sampled, six were contaminated with all three CFCs and nine were contaminated with one or two CFCs. Of the remaining 16 springs, the CFC distributions of four could be modeled assuming a single source of water, and 11 were best modeled by assuming two sources of water, with one of the water sources >60 years old. The CFC and 3H/ 3He apparent ages and the simple mixing models applied to these ages suggest that past impacts to the water quality of water recharging the sampled springs may take anywhere from 0 to ˜60 years or more to appear in the discharging spring water. In 27 springs where both 3H/ 3He ages and CFC ages were available, five springs gave similar results between the two techniques, while in the other 22 cases the 3H/ 3He apparent ages were 8-40 years greater than the CFC ages. Large excesses of 4He were observed in many of the springs, consistent with a source of older water. This older water may also carry an additional and unaccounted for source of 3He, which may be responsible for the greater 3H/ 3He ages relative to the CFC ages. We believe that the large excess 3He and 4He values and apparent age differences are related to regional climate variations because our samples were obtained at the end of a 4-year drought.

Geologic setting and geochemistry of thermal water and geothermal assessment, Trans-Pecos Texas. Final report, June 1, 1976-May 31, 1977

Energy Technology Data Exchange (ETDEWEB)

Henry, C.D.

1977-01-01

Hot springs and wells in West Texas and adjacent Mexico are manifestations of active convective geothermal systems, concentrated in a zone along the Rio Grande between the Quitman Mountains and Big Bend National Park. Maximum temperatures are 47/sup 0/ and 72/sup 0/C for hot springs and wells in Texas and 90/sup 0/C for hot springs in Mexico within 5 km of the border. Existing information is summarized and the results of a 1-year intensive study of the area are presented. The study includes several overlapping phases: (1) compilation of existing geologic information, both regional studies of geology, structure and geophysics, and more detailed local studies of individual hot spring areas; (2) detailed geologic mapping of hot spring areas to understand the origin and geologic controls of hot springs; (3) field measurement and sampling of hot spring or well waters for geochemical analysis; and (4) synthesis and interpretation of the data.

Rise and fall of road salt contamination of water-supply springs

Science.gov (United States)

Werner, Eberhard; Dipretoro, Richard S.

2006-12-01

A storage pile of de-icing agent consisting principally of sodium chloride was placed in the recharge area of two springs, and remained there for 2 years. Water flow is through fractures in rocks with low matrix permeability, along a hydraulic gradient developed along fracture zones. Salt contamination in the springs was noticed about 1 year after the salt was placed. When the salt was removed 1 year later, chloride concentrations in the springs exceeded 500 mg/L. Monitoring for the following 5 years showed salt contamination rising for the first year, but receding to normal background after 5 years. Chloride to sodium ratios of the spring waters indicated that some sodium was initially sequestered, probably by ion exchange on clay minerals, in the early part of the monitoring period, and released during the latter part; thereby extending the period of contamination.

Radon measurements in well and spring water in Lebanon

International Nuclear Information System (INIS)

Abdallah, Samer M.; Habib, Rima R.; Nuwayhid, Rida Y.; Chatila, Malek; Katul, Gabriel

2007-01-01

The variation of dissolved radon ( 222 Rn) levels in water supplies remains of interest because of the radiation-induced public health hazards. A large part of the Lebanese population relies on springs and wells for their drinking water. 222 Rn measurements in spring and well water sources were conducted using the E-PERM method at sites ranging from sea level to 1200m above sea level and across several geologic formations within Lebanon. The dissolved radon concentrations ranged from a low of 0.91BqL -1 in a coastal well source to a high of 49.6BqL -1 for a spring source in a mountainous region. Of the 20 sites sampled, only five had radon levels above 11BqL -1 and these mostly occurred in areas adjacent to well-known geological fault zones. A preliminary national average radon level was determined to be about 11.4BqL -1 . In general, as all determined concentrations were well below the 100 and 146BqL -1 revised reference levels proposed in the European Union and the United States, respectively, it is concluded that there is no reason to believe these water sources pose any radon-related hazard. On the other hand, at locations where water is collected directly from the springhead, it is advisable to have a settling/piping system installed allowing for further radon decay and radon loss into the air to alleviate any possible radon problem

Chemical and isotopic investigation of warm springs associated with normal faults in Utah

Science.gov (United States)

Cole, David R.

1983-04-01

Thermal springs associated with normal faults in Utah have been analyzed for major cations and anions, and oxygen and hydrogen isotopes. Springs with measured temperatures averaging greater than 40°C are characterized by Na + K- and SO 4 + Cl-rich waters containing 10 3 to 10 4 mg/l of dissolved solids. Lower temperature springs, averaging less than 40°C, are more enriched in Ca + Mg relative to Na + K. Chemical variations monitored through time in selected thermal springs are probably produced by mixing with non-thermal waters. During the summer months at times of maximum flow, selected hot springs exhibit their highest temperatures and maximum enrichments in most chemical constituents. Cation ratios and silica concentrations remain relatively constant through time for selected Utah thermal springs assuring the applicability of the geothermometer calculations regardless of the time of year. Geothermometer calculations utilizing either the quartz (no steam loss), chalcedony or Mg-corrected Na/K/Ca methods indicate that most thermal springs in Utah associated with normal faults have subsurface temperatures in the range of 25 to less than 120°C. This temperature range suggests fluid circulation is restricted to depths less than about three kilometers assuming an average thermal gradient of about 40°C/km. Thermodynamic calculations suggest that most thermal springs are oversaturated with respect to calcite, quartz, pyrophyllite, (Fe, Mg)-montmorillonite, microcline and hematite, and undersaturated with respect to anhydrite, gypsum, fluorite and anorthite. Chalcedony and cristobalite appear to be the only phases consistently at or near saturation in most waters. Theoretical evaluation of mixing on mineral saturation trends indicates that anhydrite and calcite become increasingly more undersaturated as cold, dilute groundwater mixes with a hot (150°C), NaCl-rich fluid. The evolution of these thermal waters issuing from faults appears to be one involving the

Recent drilling activities at the earth power resources Tuscarora geothermal power project's hot sulphur springs lease area.

Energy Technology Data Exchange (ETDEWEB)

Goranson, Colin

2005-03-01

Earth Power Resources, Inc. recently completed a combined rotary/core hole to a depth of 3,813 feet at it's Hot Sulphur Springs Tuscarora Geothermal Power Project Lease Area located 70-miles north of Elko, Nevada. Previous geothermal exploration data were combined with geologic mapping and newly acquired seismic-reflection data to identify a northerly tending horst-graben structure approximately 2,000 feet wide by at least 6,000 feet long with up to 1,700 feet of vertical offset. The well (HSS-2) was successfully drilled through a shallow thick sequence of altered Tertiary Volcanic where previous exploration wells had severe hole-caving problems. The ''tight-hole'' drilling problems were reduced using drilling fluids consisting of Polymer-based mud mixed with 2% Potassium Chloride (KCl) to reduce Smectite-type clay swelling problems. Core from the 330 F fractured geothermal reservoir system at depths of 2,950 feet indicated 30% Smectite type clays existed in a fault-gouge zone where total loss of circulation occurred during coring. Smectite-type clays are not typically expected at temperatures above 300 F. The fracture zone at 2,950 feet exhibited a skin-damage during injection testing suggesting that the drilling fluids may have caused clay swelling and subsequent geothermal reservoir formation damage. The recent well drilling experiences indicate that drilling problems in the shallow clays at Hot Sulphur Springs can be reduced. In addition, average penetration rates through the caprock system can be on the order of 25 to 35 feet per hour. This information has greatly reduced the original estimated well costs that were based on previous exploration drilling efforts. Successful production formation drilling will depend on finding drilling fluids that will not cause formation damage in the Smectite-rich fractured geothermal reservoir system. Information obtained at Hot Sulphur Springs may apply to other geothermal systems developed in

Wide distribution of autochthonous branched glycerol dialkyl glycerol tetraethers (bGDGTs in U.S. Great Basin hot springs

Directory of Open Access Journals (Sweden)

Brian P. Hedlund

2013-08-01

Full Text Available Branched glycerol dialkyl glycerol tetraethers (bGDGTs are membrane-spanning lipids that likely stabilize membranes of some bacteria. Although bGDGTs have been reported previously in certain geothermal environments, it has been suggested that they may derive from surrounding soils since bGDGTs are known to be produced by soil bacteria. To test the hypothesis that bGDGTs can be produced by thermophiles in geothermal environments, we examined the distribution and abundance of bGDGTs, along with extensive geochemical data, in 40 sediment and mat samples collected from geothermal systems in the U.S. Great Basin (temperature: 31-95°C; pH: 6.8-10.7. bGDGTs were found in 38 out of 40 samples at concentrations up to 824 ng/g sample dry mass and comprised up to 99.5% of total GDGTs (branched plus isoprenoidal. The wide distribution of bGDGTs in hot springs, strong correlation between core and polar lipid abundances, distinctness of bGDGT profiles compared to nearby soils, and higher concentration of bGDGTs in hot springs compared to nearby soils provided evidence of in situ production, particularly for the minimally methylated bGDGTs I, Ib, and Ic. Polar bGDGTs were found almost exclusively in samples ≤ 70°C and the absolute abundance of polar bGDGTs correlated negatively with properties of chemically reduced, high temperature spring sources (temperature, H2S/HS- and positively with properties of oxygenated, low temperature sites (O2, NO3-. Two-way cluster analysis and nonmetric multidimensional scaling based on relative abundance of polar bGDGTs supported these relationships and showed a negative relationship between the degree of methylation and temperature, suggesting a higher abundance for minimally methylated bGDGTs at high temperature. This study presents evidence of the widespread production of bGDGTs in mats and sediments of natural geothermal springs in the U.S. Great Basin, especially in oxygenated, low-temperature sites (≤ 70°C.

Wide distribution of autochthonous branched glycerol dialkyl glycerol tetraethers (bGDGTs) in U.S. Great Basin hot springs

Science.gov (United States)

Hedlund, Brian P.; Paraiso, Julienne J.; Williams, Amanda J.; Huang, Qiuyuan; Wei, Yuli; Dijkstra, Paul; Hungate, Bruce A.; Dong, Hailiang; Zhang, Chuanlun L.

2013-01-01

Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are membrane-spanning lipids that likely stabilize membranes of some bacteria. Although bGDGTs have been reported previously in certain geothermal environments, it has been suggested that they may derive from surrounding soils since bGDGTs are known to be produced by soil bacteria. To test the hypothesis that bGDGTs can be produced by thermophiles in geothermal environments, we examined the distribution and abundance of bGDGTs, along with extensive geochemical data, in 40 sediment and mat samples collected from geothermal systems in the U.S. Great Basin (temperature: 31–95°C; pH: 6.8–10.7). bGDGTs were found in 38 out of 40 samples at concentrations up to 824 ng/g sample dry mass and comprised up to 99.5% of total GDGTs (branched plus isoprenoidal). The wide distribution of bGDGTs in hot springs, strong correlation between core and polar lipid abundances, distinctness of bGDGT profiles compared to nearby soils, and higher concentration of bGDGTs in hot springs compared to nearby soils provided evidence of in situ production, particularly for the minimally methylated bGDGTs I, Ib, and Ic. Polar bGDGTs were found almost exclusively in samples ≤70°C and the absolute abundance of polar bGDGTs correlated negatively with properties of chemically reduced, high temperature spring sources (temperature, H2S/HS−) and positively with properties of oxygenated, low temperature sites (O2, NO−3). Two-way cluster analysis and nonmetric multidimensional scaling based on relative abundance of polar bGDGTs supported these relationships and showed a negative relationship between the degree of methylation and temperature, suggesting a higher abundance for minimally methylated bGDGTs at high temperature. This study presents evidence of the widespread production of bGDGTs in mats and sediments of natural geothermal springs in the U.S. Great Basin, especially in oxygenated, low-temperature sites (≤70°C). PMID:23964271

Isolation and characterization of a radiation resistant thermophilic bacterium from radon hot spring

International Nuclear Information System (INIS)

Liang Xinle; Yang Long; Zhang Hong; Zhang Lei

2011-01-01

A radiation resistant and thermophilic bacterium strain R4-33 was isolated from radon hot spring water samples, pretreated with 60 Co γ-rays and UV irradiation. Tests on morphological, physiological and biochemical characters, fatty acid compositions, (G + C) mol% contents, and 16S rDNA sequencing were conducted. The results showed that strain R4-33 was of rod-shape, Gram-negative, atrichous, and endospore-forming. The optimum growth temperature and pH were 60 ℃ and 7.5, respectively. The strain utilized glucose, maltose and trehalose as carbon sources, and hydrolyzed casein and starch. Its catalase positive. The strain was sensitive to penicillin, neomycin, erythromycin, vancomycin, streptomycin, gentamycin, amikacin and ampicillin. The major cellular fatty acids were C 14:1 (48.8%) and C 15:1 (15.2%). The (G + C) mol% content of DNA was 58.2%. Phylogenetic tree based on 16S rDNA sequence showed R4-33 shared highly similarity to those of species in genus Anoxybacillus, especially to that of Anoxybacillus gonensis (99.5%). Based on the above, the strain R4-33 was proposed to the evolution branch of Anoxybacillus and designated as Anoxybacillu sp. R4-33. The UV and γ-radiation tests showed that the strain R4-33 had an ability of resistance to UV of 396 J/m 2 and 60 Co γ-rays irradiation of 14.0 kGy, indicating that the strain was a radiation resistant and thermophilic bacterium. (authors)

Microbial Communities of Terrestrial Springs in Extensional Settings of the Western U.S.A.

Science.gov (United States)

Takacs-Vesbach, C.; Hall, J.; Crossey, L. J.; Karlstrom, K. E.; Fischer, T.; Cron, B.

2008-12-01

Gas and water chemistry from hot springs, gas vents, travertine-bearing cool springs, and high-pCO2 groundwaters of the western U.S. indicate a regionally extensive flux of deeply sourced volatiles through spring vents. We use the term "continental smokers" to emphasize and test analogs to mid-ocean vent systems, and are currently concentrating on vents in the Rocky Mountain/Colorado Plateau region as part of the Colorado Rockies and Experiment and Seismic transects (CREST). Measurable mantle-derived helium components (3He/4He = 0.10 to 2.1 RA) occur in nearly all springs in Colorado and New Mexico suggesting direct fast fluid pathways from the mantle to the surface hydrologic system. Important components of the CO2 are also derived from the mantle. We surveyed the geochemistry and microbial diversity of more than forty deeply sourced terrestrial springs that ranged in temperatures from 9° to 70°C. We hypothesize that degassing in continental extensional settings supports microbial assemblages that are analogous to chemolithotrophic communities at mid-ocean ridges and continental volcanic hydrothermal systems. Geochemical characteristics of the fluids and gases structure and sustain distinctive geomicrobiological communities as indicated by the widespread presence of archaea and thermophilic organisms in cool as well as hot springs.

Development of hot water utilizing power plant in fiscal 1998. Development of a binary cycle power generation plant (development of a 10-MW class plant); 1998 nendo nessui riyo hatsuden plant nado kaihatsu. Binary cycle hatsuden plant no kaihatsu (10MW kyu plant no kaihatsu)

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-01

This paper summarizes the achievements in fiscal 1998 on developing a 10-MW geothermal power plant in the Hohi-Sugawara area being a representative area of middle-to-high temperature hot water resources. In designing the plant, domestic and overseas surveys were carried out on media suitable for binary cycle power plants, thermal cycle characteristics, construction cost, environmental effects, safety, operation, maintenance and control. Latest technologies were also surveyed and analyzed. The plant construction performed development construction around the testing devices, new construction of a plant control room building, constructions for installing electrical machines including the hot water system testing devices, river water intake facility construction, and cooling water intake facility installing construction. The environmental effect investigation included investigations on rain falls, river flow rates, hot springs, spring water, monitoring during the construction, and the state of transplantation of precious plants, and observation on groundwater variation. In verifying the geothermal water pumping system, factory tests were carried out on DHP3 demonstration machine which couples the pump section of a down-hole pump with the motor section, whose performance and functions were verified. (NEDO)

Bottled water, spas, and early years of water chemistry

Science.gov (United States)

Back, William; Landa, Edward R.; Meeks, Lisa

1995-01-01

Although hot springs have been used and enjoyed for thousands of years, it was not until the late 1700s that they changed the course of world civilization by being the motivation for development of the science of chemistry. The pioneers of chemistry such as Priestley, Cavendish, Lavoisier, and Henry were working to identify and generate gases, in part, to determine their role in carbonated beverages. In the 18th century, spas in America were developed to follow the traditional activities of popular European spas. However, they were to become a dominant political and economic force in American history on three major points: (1) By far the most important was to provide a place for the leaders of individual colonies to meet and discuss the need for separation from England and the necessity for the Revolutionary War; (2) the westward expansion of the United States was facilitated by the presence of hot springs in many locations that provided the economic justification for railroads and settlement; and (3) the desire for the preservation of hot springs led to the establishment of the National Park Service. Although mineral springs have maintained their therapeutic credibility in many parts of the world, they have not done so in the United States. We suggest that the American decline was prompted by: (1) the establishment of The Johns Hopkins School of Medicine in 1893; (2) enactment of the Pure Food and Drug Act of 1907; and (3) the remarkable achievement of providing safe water supplies for American cities by the end of the 1920s. The current expanding market for bottled water is based in part on bottled water being an alternative beverage Ito alcohol and sweetened drinks and the inconsistent palatability and perceived health hazards of some tap waters.

Contribution of hot spring bacterial consortium in cadmium and lead bioremediation through quadratic programming model

Energy Technology Data Exchange (ETDEWEB)

Sen, Sudip Kumar; Raut, Sangeeta; Dora, Tapas Kumar [Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Rayagada 765 022, Odisha (India); Mohapatra, Pradeep Kumar Das, E-mail: pkdmvu@gmail.com [Department of Microbiology, Vidyasagar University, Midnapore 721 102, West Bengal (India)

2014-01-30

Highlights: • Adsorption of cadmium and lead using hot spring microbial consortium. • Development of empirical models for % adsorption using ANOVA and response surface methodology. • Fitting of the kinetics of adsorption to Freundlich and Langmuir model. • Optimization of the operating parameters to maximize the % of adsorption. -- Abstract: In the present investigation, a number of experiments have been conducted to isolate microbial strains from Taptapani Hot Spring Odisha, India for bioremediation of cadmium and lead. The strains Stenotrophomonas maltophilia (SS1), Aeromonas veronii (SS2) and Bacillus barbaricus (SS3) have shown better adaptation to metal tolerance test, with different concentrations of cadmium and lead and hence have been selected for further studies of metal microbial interaction and optimization. The results of bioremediation process indicate that consortium of thermophilic isolates adsorbed heavy metals more effectively than the individually treated isolates. Therefore, A 24 full factorial central composite design has been employed to analyze the effect of metal ion concentration, microbial concentration and time on removal of heavy metals with consortium. Analysis of variance (ANOVA) shows a high coefficient of determination value. The kinetic data have been fitted to pseudo-first order and second-order models. The isotherm equilibrium data have been well fitted by the Langmuir and Freundlich models. The optimum removal conditions determined for initial ion concentration was 0.3 g/l; contact time 72 h; microbial concentration, 3 ml/l; and pH 7. At optimum adsorption conditions, the adsorption of cadmium and lead are found to be 92% and 93%, respectively, and presence of metals was confirmed through EDS analysis.

Contribution of hot spring bacterial consortium in cadmium and lead bioremediation through quadratic programming model

International Nuclear Information System (INIS)

Sen, Sudip Kumar; Raut, Sangeeta; Dora, Tapas Kumar; Mohapatra, Pradeep Kumar Das

2014-01-01

Highlights: • Adsorption of cadmium and lead using hot spring microbial consortium. • Development of empirical models for % adsorption using ANOVA and response surface methodology. • Fitting of the kinetics of adsorption to Freundlich and Langmuir model. • Optimization of the operating parameters to maximize the % of adsorption. -- Abstract: In the present investigation, a number of experiments have been conducted to isolate microbial strains from Taptapani Hot Spring Odisha, India for bioremediation of cadmium and lead. The strains Stenotrophomonas maltophilia (SS1), Aeromonas veronii (SS2) and Bacillus barbaricus (SS3) have shown better adaptation to metal tolerance test, with different concentrations of cadmium and lead and hence have been selected for further studies of metal microbial interaction and optimization. The results of bioremediation process indicate that consortium of thermophilic isolates adsorbed heavy metals more effectively than the individually treated isolates. Therefore, A 24 full factorial central composite design has been employed to analyze the effect of metal ion concentration, microbial concentration and time on removal of heavy metals with consortium. Analysis of variance (ANOVA) shows a high coefficient of determination value. The kinetic data have been fitted to pseudo-first order and second-order models. The isotherm equilibrium data have been well fitted by the Langmuir and Freundlich models. The optimum removal conditions determined for initial ion concentration was 0.3 g/l; contact time 72 h; microbial concentration, 3 ml/l; and pH 7. At optimum adsorption conditions, the adsorption of cadmium and lead are found to be 92% and 93%, respectively, and presence of metals was confirmed through EDS analysis

Solar heating and hot water system installed at office building, One Solar Place, Dallas, Texas

Science.gov (United States)

1980-01-01

A solar heating on cooling system is described which is designed to provide 87 percent of the space heating needs, 100 percent of the potable hot water needs and is sized for future absorption cooling. The collection subsystem consists of 28 solargenics, series 76, flat plate collectors with a total area of 1,596 square feet. The solar loop circulates an ethylene glyco water solution through the collectors into a hot water system exchanger. The water storage subsystem consists of a heat exchanger, two 2,300 gallon concrete hot water storage tanks with built in heat exchangers and a back-up electric boiler. The domestic hot water subsystem sends hot water to the 10,200 square feet floor area office building hot water water fixtures. The building cold water system provides make up to the solar loop, the heating loop, and the hot water concrete storage tanks. The design, construction, cost analysis, operation and maintenance of the solar system are described.

Enrichment of Thermophilic Ammonia-Oxidizing Archaea from an Alkaline Hot Spring in the Great Basin, USA

Science.gov (United States)

Zhang, C.; Huang, Z.; Jiang, H.; Wiegel, J.; Li, W.; Dong, H.

2010-12-01

One of the major advances in the nitrogen cycle is the recent discovery of ammonia oxidation by archaea. While culture-independent studies have revealed occurrence of ammonia-oxidizing archaea (AOA) in nearly every surface niche on earth, most of these microorganisms have resisted isolation and so far only a few species have been identified. The Great Basin contains numerous hot springs, which are characterized by moderately high temperature (40-65 degree C) and circumneutral or alkaline pH. Unique thermophilic archaea have been identified based on molecular DNA and lipid biomarkers; some of which may be ammonia oxidizers. This study aims to isolate some of these archaea from a California hot spring that has pH around 9.0 and temperature around 42 degree C. Mat material was collected from the spring and transported on ice to the laboratory. A synthetic medium (SCM-5) was inoculated with the mat material and the culture was incubated under varying temperature (35-65 degree C) and pH (7.0-10.0) conditions using antibiotics to suppress bacterial growth. Growth of the culture was monitored by microscopy, decrease in ammonium and increase in nitrite, and increases in Crenarchaeota and AOA abundances over time. Clone libraries were constructed to compare archaeal community structures before and after the enrichment experiment. Temperature and pH profiles indicated that the culture grew optimally at pH 9.0 and temperature 45 degree C, which are consistent with the geochemical conditions of the natural environment. Phylogenetic analysis showed that the final OTU was distantly related to all known hyperthermophilic archaea. Analysis of the amoA genes showed two OTUs in the final culture; one of them was closely related to Candidatus Nitrososphaera gargensis. However, the enrichment culture always contained bacteria and attempts to separate them from archaea have failed. This highlights the difficulty in bringing AOA into pure culture and suggests that some of the AOA may

Spatiotemporal dynamics of spring and stream water chemistry in a high-mountain area

International Nuclear Information System (INIS)

Zelazny, Miroslaw; Astel, Aleksander; Wolanin, Anna; Malek, Stanislaw

2011-01-01

The present study deals with the application of the self-organizing map (SOM) technique in the exploration of spatiotemporal dynamics of spring and stream water samples collected in the Chocholowski Stream Basin located in the Tatra Mountains (Poland). The SOM-based classification helped to uncover relationships between physical and chemical parameters of water samples and factors determining the quality of water in the studied high-mountain area. In the upper part of the Chocholowski Stream Basin, located on the top of the crystalline core of the Tatras, concentrations of the majority of ionic substances were the lowest due to limited leaching. Significantly higher concentration of ionic substances was detected in spring and stream samples draining sedimentary rocks. The influence of karst-type springs on the quality of stream water was also demonstrated. - Highlights: → We use SOM approach to explore physiochemical data for mountain waters. → Geologic structure and hydrological events impact water chemistry. → Limited leaching, typical of crystalline core, reflects in low water mineralization. → Sedimentary rocks are susceptible for leaching. → Eutrophication has not been shown to be a threat in the Chocholowska Valley. - Spatiotemporal dynamics of spring and stream water chemistry in unique high-mountain area was evaluated by the self-organizing map technique.

Hydrograph monitoring and analysis for sustainable karst water management in Nyadeng Spring, East Borneo

Science.gov (United States)

Widyastuti, M.; Fatchurohman, H.; Fathoni, W. A.; Hakim, A. A.; Haryono, E.

2018-04-01

Karst aquifer stores abundant water resources within its matrix, conduits, and intergranular pores. Karst aquifer plays an important role in providing water supply, especially in the areas nearby that commonly dry and lack of surface water resources. Karst spring hydrograph analysis is very fundamental step to–assess and determines the condition of the catchment area in karst terrain. Recession curve is believed to be the most stable part in single flood hydrograph that represents the aquifer characteristics. Nyadeng is one of the most significant karst springs that located in Merabu Karst Area, East Borneo. Villagers in Merabu highly depend on Nyadeng Spring for fulfilled their freshwater need. Hydrograph monitoring has been initiated for one year in Nyadeng Spring as a preliminary action for karst water management in Merabu. Water level data series obtained using automatic water level data logger and then correlated with manual discharge measurement to generate stage-discharge rating curve. The stage-discharge rating curve formula for Nyadeng Spring calculated as y = 0,0102e5,8547x with r2 value = 0.8759. From the combination of several single flood events, Master Recession Curve (MRC) was generated to determine flow regime as the main consideration for karstification degree calculation. From the MRC result, flow regimes formula determined as Qt = 3.2-0.001t + 1.2(1-0.012t)+1.6(1-0.035t) indicated that one sub-regime with laminar flow and two sub-regimes with turbulent flow existed. From the MRC formula, the degree of karstification in Nyadeng Spring classified at seventh scale (developed karstification of the aquifer) based on Malik’s karstification degree (2012). The degree of karstification in Nyadeng Spring indicates that the aquifer formed by large conduit channels, fissures, and macro fissures which are able to provide significant water sources that can be utilized for multi purposes. Therefore, it is concluded that spring hydrograph monitoring provide

Hydrology of the coastal springs ground-water basin and adjacent parts of Pasco, Hernando, and Citrus Counties, Florida

Science.gov (United States)

Knochenmus, Lari A.; Yobbi, Dann K.

2001-01-01

The coastal springs in Pasco, Hernando, and Citrus Counties, Florida consist of three first-order magnitude springs and numerous smaller springs, which are points of substantial ground-water discharge from the Upper Floridan aquifer. Spring flow is proportional to the water-level altitude in the aquifer and is affected primarily by the magnitude and timing of rainfall. Ground-water levels in 206 Upper Floridan aquifer wells, and surface-water stage, flow, and specific conductance of water from springs at 10 gaging stations were measured to define the hydrologic variability (temporally and spatially) in the Coastal Springs Ground-Water Basin and adjacent parts of Pasco, Hernando, and Citrus Counties. Rainfall at 46 stations and ground-water withdrawals for three counties, were used to calculate water budgets, to evaluate long-term changes in hydrologic conditions, and to evaluate relations among the hydrologic components. Predictive equations to estimate daily spring flow were developed for eight gaging stations using regression techniques. Regression techniques included ordinary least squares and multiple linear regression techniques. The predictive equations indicate that ground-water levels in the Upper Floridan aquifer are directly related to spring flow. At tidally affected gaging stations, spring flow is inversely related to spring-pool altitude. The springs have similar seasonal flow patterns throughout the area. Water-budget analysis provided insight into the relative importance of the hydrologic components expected to influence spring flow. Four water budgets were constructed for small ground-water basins that form the Coastal Springs Ground-Water Basin. Rainfall averaged 55 inches per year and was the only source of inflow to the Basin. The pathways for outflow were evapotranspiration (34 inches per year), runoff by spring flow (8 inches per year), ground-water outflow from upward leakage (11 inches per year), and ground-water withdrawal (2 inches per year

Determination of groundwater travel time in a karst aquifer by stable water isotopes, Tanour and Rasoun spring (Jordan)