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Sample records for hormone-supplemented serum-free medium

  1. Growth in serum-free medium improves isolation of Chlamydia pneumoniae.

    OpenAIRE

    Maass, M; Essig, A; Marre, R; Henkel, W

    1993-01-01

    Infectivity titers were determined for eight Chlamydia pneumoniae strains simultaneously grown in serum-free and serum-supplemented cell culture media. Use of serum-free medium resulted in a 10- to 50-fold increase in the susceptibility of HL cells to chlamydial infection. Comparative primary isolation of a wild-type strain also produced higher inclusion counts in a serum-free environment. Serum-free cultivation is recommended to increase the efficiency of C. pneumoniae isolation from clinica...

  2. A procedure for culturing rat neocortex explants in a serum-free nutrient medium

    NARCIS (Netherlands)

    Romijn, H. J.; de Jong, B. M.; Ruijter, J. M.

    1988-01-01

    A procedure is described for long-term culturing of rat neocortex explants in a serum-free growth medium. Slices spanning the entire cortical depth from pial to ventricular side are prepared from 6-day-old rat pups. After preincubation in Hanks' balanced salt solution with extra glucose, the

  3. Rabbit chondrocytes maintained in serum-free medium. I. Synthesis and secretion of hydrodynamically-small proteoglycans

    International Nuclear Information System (INIS)

    Malemud, C.J.; Papay, R.S.

    1986-01-01

    The biosynthesis of sulfated proteoglycan in vitro by rabbit articular chondrocytes in first passage monolayer culture maintained in fetal bovine serum (FBS) or in serum-free conditions was compared. Neosynthesized proteoglycan in the culture medium in the most dense fraction of an associative CsCl density gradient (fraction dAl) declined with increasing time under serum-free conditions, but not when cells were maintained in the presence of serum. After one day, the major peak of incorporated 35 SO 4 in medium fraction dAl eluted as a retarded peak on Sepharose CL-2B, whether cells were maintained under serum-free or serum-containing conditions. The hydrodynamic size of proteoglycan monomer fraction dAlDl obtained after one day of exposure to serum-free culture media was smaller than dAlDl from serum-containing cultures. The hydrodynamic size of dAlDl obtained from serum-free culture media became even progressively smaller after 2 and 3 days' exposure to these conditions. Hydrodynamically small sulfated proteoglycans were identified in the cell-associated dAlDl fraction as early as one day after switching chondrocytes from serum-containing to serum-free medium. Proteoglycan monomer from serum-containing medium reaggregated more efficiently under both conditions. No change in the size of glycosaminoglycan chains was seen in the smaller proteoglycan subpopulations, nor was there any indication of marked changes in the glycosaminoglycan types

  4. Human dental pulp stem cells cultured in serum-free supplemented medium

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    Virginie eBonnamain

    2013-12-01

    Full Text Available Growing evidence show that human dental pulp stem cells (DPSCs could provide a source of adult stem cells for the treatment of neurodegenerative pathologies. In this study, DPSCs were expanded and cultured with a protocol generally used for the culture of neural stem/progenitor cells.Methodology: DPSC cultures were established from third molars. The pulp tissue was enzymatically digested and cultured in serum-supplemented basal medium for 12 hours. Adherent (ADH and non-adherent (non-ADH cell populations were separated according to their differential adhesion to plastic and then cultured in serum-free defined N2 medium with epidermal growth factor (EGF and basic fibroblast growth factor (bFGF. Both ADH and non-ADH populations were analyzed by FACS and/or PCR.Results: FACS analysis of ADH-DPSCs revealed the expression of the mesenchymal cell marker CD90, the neuronal marker CD56, the transferrin receptor CD71, and the chemokine receptor CXCR3, whereas hematopoietic stem cells markers CD45, CD133 and CD34 were not expressed. ADH-DPSCs expressed transcripts coding for the Nestin gene, whereas expression levels of genes coding for the neuronal markers β-III tubulin and NF-M, and the oligodendrocyte marker PLP-1 were donor dependent. ADH-DPSCs did not express the transcripts for GFAP, an astrocyte marker. Cells of the non-ADH population that grew as spheroids expressed Nestin, β-III tubulin, NF-M and PLP-1 transcripts. DPSCs migrated out of the spheroids exhibited an odontoblast-like morphology and expressed a higher level of DSPP and osteocalcin transcripts than ADH-DPSCs. Conclusion: Collectively, these data indicate that human DPSCs can be expended and cultured in serum-free supplemented medium with EGF and bFGF. ADH-DPSCs and non-ADH populations contained neuronal and/or oligodendrocyte precursors at different stages of commitment and interestingly, cells from spheroid structures seem to be more engaged into the odontoblastic lineage than the

  5. Immunohistochemical expression of stem cell, endothelial cell, and chemosensitivity markers in primary glioma spheroids cultured in serum-containing and serum-free medium

    DEFF Research Database (Denmark)

    Christensen, Karina; Aaberg-Jessen, Charlotte; Andersen, Claus

    2010-01-01

    To investigate the influence of serum-free medium (SFM) supplemented with epidermal growth factor and basic fibroblast growth factor compared with conventional serum-containing medium (SCM) on the phenotype of organotypic primary spheroids from seven gliomas.......To investigate the influence of serum-free medium (SFM) supplemented with epidermal growth factor and basic fibroblast growth factor compared with conventional serum-containing medium (SCM) on the phenotype of organotypic primary spheroids from seven gliomas....

  6. Defined culture medium for stem cell differentiation: applicability of serum-free conditions in the mouse embryonic stem cell test.

    Science.gov (United States)

    Riebeling, Christian; Schlechter, Katharina; Buesen, Roland; Spielmann, Horst; Luch, Andreas; Seiler, Andrea

    2011-06-01

    The embryonic stem cell test (EST) is a validated method to assess the developmental toxicity potency of chemicals. It was developed to reduce animal use and allow faster testing for hazard assessment. The cells used in this method are maintained and differentiated in media containing foetal calf serum. This animal product is of considerable variation in quality, and individual batches require extensive testing for their applicability in the EST. Moreover, its production involves a large number of foetuses and possible animal suffering. We demonstrate the serum-free medium and feeder cell-free maintenance of the mouse embryonic stem cell line D3 and investigate the use of specific growth factors for induction of cardiac differentiation. Using a combination of bone morphogenetic protein-2, bone morphogenetic protein-4, activin A and ascorbic acid, embryoid bodies efficiently differentiated into contracting myocardium. Additionally, examining levels of intracellular marker proteins by flow cytometry not only confirmed differentiation into cardiomyocytes, but demonstrated significant differentiation into neuronal cells in the same time frame. Thus, this approach might allow for simultaneous detection of developmental effects on both early mesodermal and neuroectodermal differentiation. The serum-free conditions for maintenance and differentiation of D3 cells described here enhance the transferability and standardisation and hence the performance of the EST. Copyright © 2011 Elsevier Ltd. All rights reserved.

  7. Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

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    Lee Gyun

    2010-09-01

    Full Text Available Abstract Background Serum-containing medium (SCM, which has a number of poorly defined components with varying concentrations, hampers standardization of lymphocyte cultures. In order to develop a serum-free medium (SFM for the expansion of human lymphocytes from peripheral blood mononuclear cells (PBMCs, a statistical optimization approach based on a fractional factorial method and a response surface method was adopted. A basal medium was prepared by supplementing RPMI1640 medium with insulin, albumin, ferric citrate, ethanolamine, fatty acids, glutamine, sodium pyruvate, 2-mercaptoethanol, 1-thioglycerol, nonessential amino acids, and vitamins. We identified additional positive determinants and their optimal concentrations for cell growth through a statistical analysis. Results From a statistical analysis using the fractional factorial method, cholesterol and polyamine supplement were identified as positive determinants for cell growth. Their optimal concentrations were determined by the response surface method. The maximum viable cell concentration in the developed SFM was enhanced by more than 1.5-fold when compared to that in RPMI1640 supplemented with 10% fetal bovine serum (FBS. Furthermore, a cytotoxicity assay and an enzyme-linked immunospot assay revealed that the effector function of cytotoxic T lymphocytes generated from PBMCs grown in SFM, by stimulation of peptide-presenting dendritic cells, was retained or even better than that in SCM. Conclusions The use of a developed SFM with cholesterol and polyamine supplement for human lymphocyte culture resulted in better growth without loss of cellular function when compared to SCM.

  8. Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design.

    Science.gov (United States)

    Jeon, Min Kyoung; Lim, Jong-Baeck; Lee, Gyun Min

    2010-09-21

    Serum-containing medium (SCM), which has a number of poorly defined components with varying concentrations, hampers standardization of lymphocyte cultures. In order to develop a serum-free medium (SFM) for the expansion of human lymphocytes from peripheral blood mononuclear cells (PBMCs), a statistical optimization approach based on a fractional factorial method and a response surface method was adopted. A basal medium was prepared by supplementing RPMI1640 medium with insulin, albumin, ferric citrate, ethanolamine, fatty acids, glutamine, sodium pyruvate, 2-mercaptoethanol, 1-thioglycerol, nonessential amino acids, and vitamins. We identified additional positive determinants and their optimal concentrations for cell growth through a statistical analysis. From a statistical analysis using the fractional factorial method, cholesterol and polyamine supplement were identified as positive determinants for cell growth. Their optimal concentrations were determined by the response surface method. The maximum viable cell concentration in the developed SFM was enhanced by more than 1.5-fold when compared to that in RPMI1640 supplemented with 10% fetal bovine serum (FBS). Furthermore, a cytotoxicity assay and an enzyme-linked immunospot assay revealed that the effector function of cytotoxic T lymphocytes generated from PBMCs grown in SFM, by stimulation of peptide-presenting dendritic cells, was retained or even better than that in SCM. The use of a developed SFM with cholesterol and polyamine supplement for human lymphocyte culture resulted in better growth without loss of cellular function when compared to SCM.

  9. Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

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    Shahrul Hisham Zainal Ariffin

    2013-01-01

    Full Text Available Dental pulp tissue contains dental pulp stem cells (DPSCs. Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teeth extracted from 6- to 8-week-old mice and maintained in complete medium. The cells from the fourth passage were induced to differentiate by culturing in medium without serum or growth factors. RT-PCR molecular analysis showed characteristics of Cd146+, Cd166+, and Cd31− in DPSCs, indicating that these cells are mesenchymal stem cells rather than hematopoietic stem cells. After 5 days of neuronal differentiation, the cells showed neuron-like morphological changes and expressed MAP2 protein. The activation of Nestin was observed at low level prior to differentiation and increased after 5 days of culture in differentiation medium, whereas Tub3 was activated only after 5 days of neuronal differentiation. The proliferation of the differentiated cells decreased in comparison to that of the control cells. Dental pulp stem cells are induced to differentiate into neuron-like cells when cultured in serum- and growth factor-free medium.

  10. Taurine Biosynthesis in a Fish Liver Cell Line (ZFL) Adapted to a Serum-Free Medium.

    Science.gov (United States)

    Liu, Chieh-Lun; Watson, Aaron M; Place, Allen R; Jagus, Rosemary

    2017-05-25

    Although taurine has been shown to play multiple important physiological roles in teleosts, little is known about the molecular mechanisms underlying dietary requirements. Cell lines can provide useful tools for deciphering biosynthetic pathways and their regulation. However, culture media and sera contain variable taurine levels. To provide a useful cell line for the investigation of taurine homeostasis, an adult zebrafish liver cell line (ZFL) has been adapted to a taurine-free medium by gradual accommodation to a commercially available synthetic medium, UltraMEM™-ITES. Here we show that ZFL cells are able to synthesize taurine and be maintained in medium without taurine. This has allowed for the investigation of the effects of taurine supplementation on cell growth, cellular amino acid pools, as well as the expression of the taurine biosynthetic pathway and taurine transporter genes in a defined fish cell type. After taurine supplementation, cellular taurine levels increase but hypotaurine levels stay constant, suggesting little suppression of taurine biosynthesis. Cellular methionine levels do not change after taurine addition, consistent with maintenance of taurine biosynthesis. The addition of taurine to cells grown in taurine-free medium has little effect on transcript levels of the biosynthetic pathway genes for cysteine dioxygenase (CDO), cysteine sulfinate decarboxylase (CSAD), or cysteamine dioxygenase (ADO). In contrast, supplementation with taurine causes a 30% reduction in transcript levels of the taurine transporter, TauT. This experimental approach can be tailored for the development of cell lines from aquaculture species for the elucidation of their taurine biosynthetic capacity.

  11. Production of inactivated influenza H5N1 vaccines from MDCK cells in serum-free medium.

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    Alan Yung-Chih Hu

    Full Text Available BACKGROUND: Highly pathogenic influenza viruses pose a constant threat which could lead to a global pandemic. Vaccination remains the principal measure to reduce morbidity and mortality from such pandemics. The availability and surging demand for pandemic vaccines needs to be addressed in the preparedness plans. This study presents an improved high-yield manufacturing process for the inactivated influenza H5N1 vaccines using Madin-Darby canine kidney (MDCK cells grown in a serum-free (SF medium microcarrier cell culture system. PRINCIPAL FINDING: The current study has evaluated the performance of cell adaptation switched from serum-containing (SC medium to several commercial SF media. The selected SF medium was further evaluated in various bioreactor culture systems for process scale-up evaluation. No significant difference was found in the cell growth in different sizes of bioreactors studied. In the 7.5 L bioreactor runs, the cell concentration reached to 2.3 × 10(6 cells/mL after 5 days. The maximum virus titers of 1024 Hemagglutinin (HA units/50 µL and 7.1 ± 0.3 × 10(8 pfu/mL were obtained after 3 days infection. The concentration of HA antigen as determined by SRID was found to be 14.1 µg/mL which was higher than those obtained from the SC medium. A mouse immunogenicity study showed that the formalin-inactivated purified SF vaccine candidate formulated with alum adjuvant could induce protective level of virus neutralization titers similar to those obtained from the SC medium. In addition, the H5N1 viruses produced from either SC or SF media showed the same antigenic reactivity with the NIBRG14 standard antisera. CONCLUSIONS: The advantages of this SF cell-based manufacturing process could reduce the animal serum contamination, the cost and lot-to-lot variation of SC medium production. This study provides useful information to manufacturers that are planning to use SF medium for cell-based influenza vaccine production.

  12. Generation of megakaryocytic progenitors from human embryonic stem cells in a feeder- and serum-free medium.

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    Marjorie Pick

    Full Text Available BACKGROUND: The production of human platelets from embryonic stem cells in a defined culture system is a prerequisite for the generation of platelets for therapeutic use. As an important step towards this goal, we report the differentiation of human embryonic stem cells (hESCs towards the megakaryocyte (Mk lineage using a 'spin embryoid body' method in serum-free differentiation medium. METHODOLOGY AND PRINCIPAL FINDINGS: Immunophenotypic analyses of differentiating hESC identified a subpopulation of cells expressing high levels of CD41a that expressed other markers associated with the Mk lineage, including CD110, CD42b and CD61. Differentiated cells were sorted on the basis of their expression of CD41a, CD34 and CD45 and assessed for Mk colony formation, expression of myeloid and Mk genes and ability to endoreplicate DNA. In a collagen-based colony assay, the CD41a⁺ cells sorted from these differentiation cultures produced 100-800 Mk progenitors at day 13 and 25-160 Mk progenitors at day 20 of differentiation per 100,000 cells assayed. Differentiated Mk cells produced platelet-like particles which expressed CD42b and were activated by ADP, similar to platelets generated from precursors in cord blood. These studies were complemented by real time PCR analyses showing that subsets of cells enriched for CD41a⁺ Mk precursors expressed high levels of Mk associated genes such as PF4 and MPL. Conversely, high levels of myeloid and erythroid related transcripts, such as GATA1, TAL1/SCL and PU.1, were detected in sorted fractions containing CD34⁺ and CD45⁺ cells. CONCLUSIONS: We describe a serum- and feeder-free culture system that enabled the generation of Mk progenitors from human embryonic stem cells. These cells formed colonies that included differentiated Mks that fragmented to form platelet-like particles. This protocol represents an important step towards the generation of human platelets for therapeutic use.

  13. The influence of serum substituents on serum-free Vero cell conditioned culture media manufactured from Dulbecco's modified Eagle medium in mouse embryo culture.

    Science.gov (United States)

    Lee, Jong-Seon; Kim, Ju-Hwan; Seo, Young-Seok; Yang, Jung-Bo; Kim, Yong-Il; Kim, Hye-Jin; Lee, Ki-Hwan

    2013-09-01

    This study was conducted to examine the influences of supplementation of the serum substituents and available period of serum-free Vero cell conditioned media (SF-VCM) manufactured from Dulbecco's modified Eagle medium cultured with Vero cells for in vitro development of mouse preimplantation embryos. A total of 1,099 two-cell embryos collected from imprinting control region mice were cultured in SF-VCM with 10% and 20% human follicular fluid (hFF), serum substitute supplement (SSS), and serum protein substitute (SPS). Development of embryos was observed every 24 hours. Results between different groups were analyzed by chi-square test, and considered statistically significant when P-value was less than 0.05. The rates of embryonic development cultured in SF-VCM supplemented with serum substituents were significantly higher compare with serum-free group (P media up to 4 weeks did not affect on embryonic development.

  14. Bovine babesiosis: Cattle protected in the field with a frozen vaccine containing Babesia bovis and Babesia bigemina cultured in vitro with a serum-free medium.

    Science.gov (United States)

    Rojas-Martínez, Carmen; Rodríguez-Vivas, Roger Iván; Millán, Julio Vicente Figueroa; Bautista-Garfias, Carlos Ramón; Castañeda-Arriola, Roberto Omar; Lira-Amaya, José Juan; Urióstegui, Patricia Vargas; Carrasco, Juan José Ojeda; Martínez, Jesús Antonio Álvarez

    2018-04-01

    An attenuated live vaccine containing Babesia bovis and B. bigemina cultured in vitro with a serum-free medium was assessed for its clinical protection conferred of naïve cattle, under natural tick-challenge in a high endemicity zone to Babesia spp. Three groups of six animals were treated as follows: group I (GI) received a vaccine derived from parasites cultured with a free-serum medium; group II (GII) were immunized with the standard vaccine, with parasites cultured in a medium supplemented with 40% (v/v) bovine serum; and a control group (GIII) inoculated with non-infected bovine erythrocytes. Inocula were administered by IM route. Experimental animals were kept during 23days after vaccination in a cattle farm free of ticks and Babesia spp. Thereafter, cattle were moved to a high endemicity farm for natural exposure to Babesia spp. transmitted by Rhipicephalus microplus ticks. Protection against clinical babesiosis was observed in bovines belonging to GI (100%) and GII (83.33%), while the control animals (GIII) were not protected, and showed severe clinical signs, closely related to babesiosis, were observed for at least three consecutive days during the challenge. These were fever, anemia, which were measured simultaneously, and circulating parasites were detected by optic light microscopy. All cattle showed B. bovis and B. bigemina in stained blood films during the challenge; B. bovis antibody titers were higher than those to B. bigemina in GI and GII, and lower titers were determined in GIII. The protective capacity of the vaccine derived from B. bovis and B. bigemina cultured in vitro in a serum-free medium was demonstrated. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Bone Marrow Mesenchymal Stem Cells Enhance the Differentiation of Human Switched Memory B Lymphocytes into Plasma Cells in Serum-Free Medium

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    Guillaume Bonnaure

    2016-01-01

    Full Text Available The differentiation of human B lymphocytes into plasma cells is one of the most stirring questions with regard to adaptive immunity. However, the terminal differentiation and survival of plasma cells are still topics with much to be discovered, especially when targeting switched memory B lymphocytes. Plasma cells can migrate to the bone marrow in response to a CXCL12 gradient and survive for several years while secreting antibodies. In this study, we aimed to get closer to niches favoring plasma cell survival. We tested low oxygen concentrations and coculture with mesenchymal stem cells (MSC from human bone marrow. Besides, all cultures were performed using an animal protein-free medium. Overall, our model enables the generation of high proportions of CD38+CD138+CD31+ plasma cells (≥50% when CD40-activated switched memory B lymphocytes were cultured in direct contact with mesenchymal stem cells. In these cultures, the secretion of CXCL12 and TGF-β, usually found in the bone marrow, was linked to the presence of MSC. The level of oxygen appeared less impactful than the contact with MSC. This study shows for the first time that expanded switched memory B lymphocytes can be differentiated into plasma cells using exclusively a serum-free medium.

  16. Optimization of a serum-free culture medium for mouse embryonic stem cells using design of experiments (DoE) methodology.

    Science.gov (United States)

    Knöspel, Fanny; Schindler, Rudolf K; Lübberstedt, Marc; Petzolt, Stephanie; Gerlach, Jörg C; Zeilinger, Katrin

    2010-12-01

    The in vitro culture behaviour of embryonic stem cells (ESC) is strongly influenced by the culture conditions. Current culture media for expansion of ESC contain some undefined substances. Considering potential clinical translation work with such cells, the use of defined media is desirable. We have used Design of Experiments (DoE) methods to investigate the composition of a serum-free chemically defined culture medium for expansion of mouse embryonic stem cells (mESC). Factor screening analysis according to Plackett-Burman revealed that insulin and leukaemia inhibitory factor (LIF) had a significant positive influence on the proliferation activity of the cells, while zinc and L: -cysteine reduced the cell growth. Further analysis using minimum run resolution IV (MinRes IV) design indicates that following factor adjustment LIF becomes the main factor for the survival and proliferation of mESC. In conclusion, DoE screening assays are applicable to develop and to refine culture media for stem cells and could also be employed to optimize culture media for human embryonic stem cells (hESC).

  17. Long-term culture of rat hippocampal neurons at low density in serum-free medium: combination of the sandwich culture technique with the three-dimensional nanofibrous hydrogel PuraMatrix.

    Science.gov (United States)

    Kaneko, Ai; Sankai, Yoshiyuki

    2014-01-01

    The primary culture of neuronal cells plays an important role in neuroscience. There has long been a need for methods enabling the long-term culture of primary neurons at low density, in defined serum-free medium. However, the lower the cell density, the more difficult it is to maintain the cells in culture. Therefore, we aimed to develop a method for long-term culture of neurons at low density, in serum-free medium, without the need for a glial feeder layer. Here, we describe the work leading to our determination of a protocol for long-term (>2 months) primary culture of rat hippocampal neurons in serum-free medium at the low density of 3×10(4) cells/mL (8.9×10(3) cells/cm2) without a glial feeder layer. Neurons were cultured on a three-dimensional nanofibrous hydrogel, PuraMatrix, and sandwiched under a coverslip to reproduce the in vivo environment, including the three-dimensional extracellular matrix, low-oxygen conditions, and exposure to concentrated paracrine factors. We examined the effects of varying PuraMatrix concentrations, the timing and presence or absence of a coverslip, the timing of neuronal isolation from embryos, cell density at plating, medium components, and changing the medium or not on parameters such as developmental pattern, cell viability, neuronal ratio, and neurite length. Using our method of combining the sandwich culture technique with PuraMatrix in Neurobasal medium/B27/L-glutamine for primary neuron culture, we achieved longer neurites (≥3,000 µm), greater cell viability (≥30%) for 2 months, and uniform culture across the wells. We also achieved an average neuronal ratio of 97%, showing a nearly pure culture of neurons without astrocytes. Our method is considerably better than techniques for the primary culture of neurons, and eliminates the need for a glial feeder layer. It also exhibits continued support for axonal elongation and synaptic activity for long periods (>6 weeks).

  18. Long-term culture of rat hippocampal neurons at low density in serum-free medium: combination of the sandwich culture technique with the three-dimensional nanofibrous hydrogel PuraMatrix.

    Directory of Open Access Journals (Sweden)

    Ai Kaneko

    Full Text Available The primary culture of neuronal cells plays an important role in neuroscience. There has long been a need for methods enabling the long-term culture of primary neurons at low density, in defined serum-free medium. However, the lower the cell density, the more difficult it is to maintain the cells in culture. Therefore, we aimed to develop a method for long-term culture of neurons at low density, in serum-free medium, without the need for a glial feeder layer. Here, we describe the work leading to our determination of a protocol for long-term (>2 months primary culture of rat hippocampal neurons in serum-free medium at the low density of 3×10(4 cells/mL (8.9×10(3 cells/cm2 without a glial feeder layer. Neurons were cultured on a three-dimensional nanofibrous hydrogel, PuraMatrix, and sandwiched under a coverslip to reproduce the in vivo environment, including the three-dimensional extracellular matrix, low-oxygen conditions, and exposure to concentrated paracrine factors. We examined the effects of varying PuraMatrix concentrations, the timing and presence or absence of a coverslip, the timing of neuronal isolation from embryos, cell density at plating, medium components, and changing the medium or not on parameters such as developmental pattern, cell viability, neuronal ratio, and neurite length. Using our method of combining the sandwich culture technique with PuraMatrix in Neurobasal medium/B27/L-glutamine for primary neuron culture, we achieved longer neurites (≥3,000 µm, greater cell viability (≥30% for 2 months, and uniform culture across the wells. We also achieved an average neuronal ratio of 97%, showing a nearly pure culture of neurons without astrocytes. Our method is considerably better than techniques for the primary culture of neurons, and eliminates the need for a glial feeder layer. It also exhibits continued support for axonal elongation and synaptic activity for long periods (>6 weeks.

  19. Attachment and growth of human keratinocytes in a serum-free environment.

    Science.gov (United States)

    Gilchrest, B A; Calhoun, J K; Maciag, T

    1982-08-01

    Using a serum-free system, we have investigated the influence of human fibronectin (HFN) and selected growth factors (GF) on the attachment and growth of normal human keratinocytes in vitro. Single-cell suspensions of keratinocytes from near-confluent primary plates, plated on 5-10 microgram/cm2 HFN, showed approximately 30-40% attachment after 2-24 hours of incubation at 37 degrees C, compared with 4-6% attachment on uncoated platic plates. Percentage of attached cells was independent of seed density, tissue donor age, in vitro culture age, or medium composition, while subsequent cellular proliferation was strongly dependent on these factors. Keratinocytes grown on an adequate HFN matrix in a previously described hormone-supplemented medium (Maciag et al., 1981a) achieved four to eight population doubling over 7-12 days at densities greater than or equal to 104 cell/cm2. Removal of most GF individually from the medium had little or no effect on growth, while removal of epidermal growth factor (EGF) alone reduced growth by 30-35% and removal of bovine brain extract (BE) alone reduced growth by approximately 90%. Conversely, EGF alone in basal medium supported approximately 10% control growth, BE alone supported 30-40% control growth, and the combination of EGF and BE approximately 70%. In addition to its major effect on proliferation in this system, BE was necessary to preserve normal keratinocyte morphology and protein production. These findings expand earlier observations that HFN facilitates keratinocyte attachment in vitro and that a brain-derived extract can exert a major positive influence on cultured keratinocytes.

  20. Tumour-cytolytic human monocyte-derived macrophages: a simple and efficient method for the generation and long-term cultivation as non-adherent cells in a serum-free medium.

    Science.gov (United States)

    Streck, R J; Hurley, E L; Epstein, D A; Pauly, J L

    1992-01-01

    We report a simple and efficient culture procedure for the generation of tumour-cytolytic human monocyte-derived macrophages (MAC). In this method, normal human peripheral blood mononuclear cells, isolated using a conventional Ficoll-Hypaque density gradient procedure, are cultured as a heterogenous leukocyte population in Teflon or other hydrophobic cultureware, in a commercially available serum-free culture medium (M-SFM) that has been formulated specifically for the cultivation and ex vivo stimulation of human monocytes and MAC, and in the absence of exogenous mitogens, antigens, cytokines or other stimulants. This procedure features a negative-selection technique that takes advantage of the differential survival of blood leukocytes. Using the prescribed in vitro conditions, lymphocytes survived relatively poorly, whereas monocytes differentiated in the absence of exogenous stimulants into mature tumour-cytolytic MAC. The MAC were present as non-adherent, single cells that expressed good viability (greater than 95%) for a prolonged period (greater than 60 days). When compared to conventional procedures for generating MAC, the prescribed technique is thought to offer several important advantages in that it: (a) eliminates the tedious and cumbersome monocyte isolation procedures, thus providing a significant savings not only in time and money but also in eliminating repetitive cell manipulations that have often been associated with damage to monocyte morphology and/or function; (b) reduces the loss of monocyte subsets that are not recovered during specific isolation procedures; (c) facilitates harvesting a single cell, non-adherent suspension of immunocompetent MAC suitable for various examinations including analyses defining MAC morphology, cytochemistry, phenotype and function; and (d) eliminates variability and artifacts associated with different sera that are utilised frequently as medium supplements. The utility of the prescribed method is illustrated by the

  1. Serum-free media formulations are cell line-specific and require optimization for microcarrier culture.

    Science.gov (United States)

    Tan, Kah Yong; Teo, Kim Leng; Lim, Jessica F Y; Chen, Allen K L; Choolani, Mahesh; Reuveny, Shaul; Chan, Jerry; Oh, Steve Kw

    2015-08-01

    Mesenchymal stromal cells (MSCs) are being investigated as potential cell therapies for many different indications. Current methods of production rely on traditional monolayer culture on tissue-culture plastic, usually with the use of serum-supplemented growth media. However, the monolayer culturing system has scale-up limitations and may not meet the projected hundreds of billions to trillions batches of cells needed for therapy. Furthermore, serum-free medium offers several advantages over serum-supplemented medium, which may have supply and contaminant issues, leading to many serum-free medium formulations being developed. We cultured seven MSC lines in six different serum-free media and compared their growth between monolayer and microcarrier culture. We show that (i) expansion levels of MSCs in serum-free monolayer cultures may not correlate with expansion in serum-containing media; (ii) optimal culture conditions (serum-free media for monolayer or microcarrier culture) differ for each cell line; (iii) growth in static microcarrier culture does not correlate with growth in stirred spinner culture; (iv) and that early cell attachment and spreading onto microcarriers does not necessarily predict efficiency of cell expansion in agitated microcarrier culture. Current serum-free media developed for monolayer cultures of MSCs may not support MSC proliferation in microcarrier cultures. Further optimization in medium composition will be required for microcarrier suspension culture for each cell line. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  2. The influence of serum-free culture conditions on skeletal muscle differentiation in a tissue-engineered model

    NARCIS (Netherlands)

    Gawlitta, D.; Boonen, K.J.M.; Oomens, C.W.J.; Baaijens, F.P.T.; Bouten, C.V.C.

    2008-01-01

    The influence of differentiation medium (DM) components on C2C12 murine myoblast differentiation has only been studied in monolayer cultures. Serum-free formulations have been applied that omit the use of sera with unknown composition. The goal of the present study was to compare the influence of

  3. Development of serum-free media for lepidopteran insect cell lines.

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    Agathos, Spiros N

    2007-01-01

    Lepidopteran insect cell culture technology has progressed to the point of becoming an essential part of one of the most successful eukaryotic expression systems and is increasingly used industrially on a large scale. Therefore, there is a constant need for convenient and low-cost culture media capable of supporting good insect cell growth and ensuring high yield of baculovirus as well as the strong expression of recombinant proteins. Vertebrate sera or invertebrate hemolymph were essential supplements in first-generation insect cell media. These supplements, however, are cumbersome and expensive for routine large-scale culture; thus, their use is now circumvented by substituting the essential growth factors present in these supplements with serum-free substances. Such non-serum supplements are typically of non-animal origin and include protein hydrolysates, lipid emulsions, and specialized substances (e.g., surfactants and shear damage protecting chemicals). These supplements need to complement the defined, synthetic basal medium to ensure that the fundamental nutritional needs of the cells are satisfied. Although there is a significant number of proprietary serum-free and low-protein or protein-free media on the market, the lack of information concerning their detailed composition is a drawback in their adoption for different applications, including their adaptation to the metabolic and kinetic analysis and monitoring of a given insect cell based bioprocess. Hence, there is wide appeal for formulating serum-free media based on a rational assessment of the metabolic requirements of the lepidopteran cells during both the growth and the production phases. Techniques such as statistical experimental design and genetic algorithms adapted to the cellular behavior and the bioreactor operation mode (batch, fed-batch, or perfusion) permit the formulation of versatile serum- and protein-free media. These techniques are illustrated with recent developments of serum-free

  4. The B-domain of factor VIII reduces cell membrane attachement to host cells in serum free conditions

    DEFF Research Database (Denmark)

    Kolind, Mille Petersen; Nørby, Peder Lisby; Flintegaard, Thomas Veje

    2010-01-01

    engineered extensively throughout the years to increase the low production yields that initially were obtained from mammalian cell cultures. The scope of this work was to investigate the interaction of rFVIII with the cell membrane surface of the producing cells in serum free medium. We wondered whether...... binding of rFVIII to the cell membrane could be a factor diminishing the production yield. We studied the contribution of the rFVIII B-domain to membrane attachment by transfecting several constructs containing increasing lengths of the B-domain into cells under serum free conditions. We found that 90......% of rFVIII is attached to the cell membrane of the producing cell when the rFVIII variant contains a short B-domain (21 aa). By increasing the length of the B-domain the membrane attached fraction can be reduced to 50% of the total expressed rFVIII. Further, our studies show that the N...

  5. Newly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours

    NARCIS (Netherlands)

    Bate-Eya, Laurel T.; Ebus, Marli E.; Koster, Jan; den Hartog, Ilona J. M.; Zwijnenburg, Danny A.; Schild, Linda; van der Ploeg, Ida; Dolman, M. Emmy M.; Caron, Huib N.; Versteeg, Rogier; Molenaar, Jan J.

    2014-01-01

    Recently protocols have been devised for the culturing of cell lines from fresh tumours under serum-free conditions in defined neural stem cell medium. These cells, frequently called tumour initiating cells (TICs) closely retained characteristics of the tumours of origin. We report the isolation of

  6. Effect of IL-6 on proliferation and IG production of human EBV-transformed cell lines in serum free culture media

    NARCIS (Netherlands)

    Jochems, G. J.; Jordens, R.; van Lier, R. A.; Zeijlemaker, W. P.

    1990-01-01

    To optimalize growth and Ig production of EBV transformed B cells for large scale tissue culture, we analyzed five stable monoclonal EBV-B cell lines for their responsiveness to interleukin (IL)-6 in standard medium with 5% FCS and in several serum-free media. As we previously demonstrated these

  7. Prognostic Value of Serum Free Light Chain in Multiple Myeloma.

    Science.gov (United States)

    El Naggar, Amel A; El-Naggar, Mostafa; Mokhamer, El-Hassan; Avad, Mona W

    2015-01-01

    The measurement of serum free light chain (sFLC) has been shown to be valuable in screening for the presence of plasma cell dyscrasia as well as for baseline prognosis in newly diagnosed patients. The aim of the present work was to study the prognostic value of sFLC in multiple myeloma in relation to other serum biomarkers, response to therapy and survival. Forty five newly diagnosed patients with MM were included in the study. Patients were divided into responders and non-responders groups according to response to therapy. sFLC and serum Amyloid A (SAA) were measured by immunonephelometry. The non-responders group showed a statistically significant higher kappa/lambda or lambda/kappa ratio and higher β2 microglobulin level, but lower albumin level at presentation, as compared to the responders group (P < 0.001). However, no statistically significant difference was detected between the two groups regarding SA A or calcium levels. Comparison between sFLC ratio obtained before and after therapy revealed significant decrease after treatment in the responders group (P = 0.05). Survival was significantly inferior in patients with an FLC ratio of ≥ 2.6 or ≤ 0.56 compared with those with an FLC ratio that was between 0.56 and 2.6 (P = 0.002).

  8. Serum free light chain reference values: a critical approach.

    Science.gov (United States)

    Altinier, Sara; Seguso, Mara; Zaninotto, Martina; Varagnolo, Mariacristina; Adami, Fausto; Angeli, Paolo; Plebani, Mario

    2013-05-01

    The clinical usefulness of serum free light chain (FLC) measurement in the management of patients with plasma cell proliferative disorders has been reported in several papers, and most clinical studies use the reference ranges declared by the manufacturer. The aim of the present study was to evaluate the reproducibility of FLCs immunoassay and to validate the reference range, before introducing it in routine setting. Internal quality control materials and a pool of fresh serum samples were used to evaluate imprecision; 162 fresh sera from healthy blood donors were analyzed to evaluate the reference range for FLCs. In order to verify the κ/λ FLC ratio, 43 sera from patients with polyclonal hypergammaglobulinemia were tested. The FLC immunoassay was performed using a nephelometer with the Freelite reagents. The imprecision studies performed using a serum pool tested with two different lots of reagents showed a mean CV of 16.09% for κFLC and of 16.72% for λFLC. Lower CV%s and different mean values were found by calculating the results from each specific lot separately, while different results were obtained using the control materials provided by the manufacturer. In reference subjects, the 2.5-97.5th percentiles were found to be 4.52-22.33 and 4.84-21.88mg/L for κFLC and λFLC, respectively. The range for κ/λ ratio (0.65-2.36) was validated with the values obtained from subjects with polyclonal hypergammaglobulinemia. In retesting 15 samples from blood donor subjects with a different lot of reagents, mean bias percentages of 17.60 for κFLC and 15.26 for λFLC were obtained. These findings confirm the lot-to-lot variability of the FLC assays also in the measurement of polyclonal light chains, as well as the need to carefully validate the reference values. Published by Elsevier Inc.

  9. Serum-free Erythroid Differentiation for Efficient Genetic Modification and High-Level Adult Hemoglobin Production.

    Science.gov (United States)

    Uchida, Naoya; Demirci, Selami; Haro-Mora, Juan J; Fujita, Atsushi; Raines, Lydia N; Hsieh, Matthew M; Tisdale, John F

    2018-06-15

    In vitro erythroid differentiation from primary human cells is valuable to develop genetic strategies for hemoglobin disorders. However, current erythroid differentiation methods are encumbered by modest transduction rates and high baseline fetal hemoglobin production. In this study, we sought to improve both genetic modification and hemoglobin production among human erythroid cells in vitro . To model therapeutic strategies, we transduced human CD34 + cells and peripheral blood mononuclear cells (PBMCs) with lentiviral vectors and compared erythropoietin-based erythroid differentiation using fetal-bovine-serum-containing media and serum-free media. We observed more efficient transduction (85%-93%) in serum-free media than serum-containing media (20%-69%), whereas the addition of knockout serum replacement (KSR) was required for serum-free media to promote efficient erythroid differentiation (96%). High-level adult hemoglobin production detectable by electrophoresis was achieved using serum-free media similar to serum-containing media. Importantly, low fetal hemoglobin production was observed in the optimized serum-free media. Using KSR-containing, serum-free erythroid differentiation media, therapeutic adult hemoglobin production was detected at protein levels with β-globin lentiviral transduction in both CD34 + cells and PBMCs from sickle cell disease subjects. Our in vitro erythroid differentiation system provides a practical evaluation platform for adult hemoglobin production among human erythroid cells following genetic manipulation.

  10. Evolution of triiodothyronine nuclear binding sites in hypothalamic serum-free cultures: evidence for their presence in neurons and astrocytes

    International Nuclear Information System (INIS)

    Puymirat, J.; Faivre-Bauman, A.

    1986-01-01

    ( 125 I)Triiodothyronine (T 3 ) nuclear binding was studied in hypothalamic cultures from fetal mouse grown in serum-free medium. In enriched neuronal cultures, the apparent dissociation constant of the binding does not change with time in vitro (7 x 10 -11 M), but the maximum binding capacity (MBC) doubles between day 7 and day 14 in vitro. We show here for the first time that homologous astrocyte cell cultures, devoid of neurons as checked by tetanus toxin binding, also display T 3 nuclear binding, with the same affinity as neuronal cultures. However, their MBC is 3 times lower than that of neurons after a week in vitro, and increases more quickly thereafter (Author)

  11. Production and characterization of high-titer serum-free cell culture grown hepatitis C virus particles of genotype 1-6

    DEFF Research Database (Denmark)

    Mathiesen, Christian K; Jensen, Tanja B; Prentoe, Jannick

    2014-01-01

    Recently, cell culture systems producing hepatitis C virus particles (HCVcc) were developed. Establishment of serum-free culture conditions is expected to facilitate development of a whole-virus inactivated HCV vaccine. We describe generation of genotype 1-6 serum-free HCVcc (sf-HCVcc) from Huh7.......5 hepatoma cells cultured in adenovirus expression medium. Compared to HCVcc, sf-HCVcc showed 0.6-2.1 log10 higher infectivity titers (4.7-6.2 log10 Focus Forming Units/mL), possibly due to increased release and specific infectivity of sf-HCVcc. In contrast to HCVcc, sf-HCVcc had a homogeneous single......-peak density profile. Entry of sf-HCVcc depended on HCV co-receptors CD81, LDLr, and SR-BI, and clathrin-mediated endocytosis. HCVcc and sf-HCVcc were neutralized similarly by chronic-phase patient sera and by human monoclonal antibodies targeting conformational epitopes. Thus, we developed serum-free culture...

  12. Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State

    Directory of Open Access Journals (Sweden)

    Corsini Joe

    2004-01-01

    Full Text Available Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative. Using five mammalian lines (Crandell Feline Kidney, MCF7, A72, WI 38 and NB324K, we found that the serum-free alternative preserves nearly as efficiently as the serum-containing preservatives. Second, we compared cryostorage of those cells in suspended versus a pellet form using both aforementioned cryopreservatives. Under our conditions, cells were in general recovered equally well in a suspended versus a pellet form.

  13. Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells

    Directory of Open Access Journals (Sweden)

    Yan Mylene L

    2011-08-01

    first time the production of Influenza viruses using Vero cells in commercially available animal-component free, serum-free medium. This work can be used as a basis for efficient production of attenuated as well as wild type Influenza virus for research and vaccine production.

  14. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production.

    Science.gov (United States)

    Ferreira, Tiago B; Ferreira, Ana L; Carrondo, Manuel J T; Alves, Paula M

    2005-11-01

    Adenoviruses are promising vectors for gene therapy and vaccination protocols. Consequently, the market demands for adenovirus are increasing, driving the search for new methodologies for large-scale production of concentrated vectors with warranted purity and efficacy, in a cost-effective way. Nevertheless, the production of adenovirus is currently limited by the so-called 'cell density effect', i.e. a drop in cell specific productivity concomitant with increased cell concentration at infection. Of two different serum-free culture media (CD293 and EX-Cell), evaluated for their effect on human 293 cells growth and adenovirus production at cell densities higher than 1x10(6) cells/ml, EX-Cell proved the better medium for cell growth. Although adenovirus production was equivalent in both media when the infection was performed at 1x10(6) cells/ml, at 3x10(6) cells/ml CD293 was the better. This result related to the high ammonia content in EX-Cell medium at the highest cell concentration at infection. Besides this, the large-scale production of these vectors at high cell densities often requires re-feed strategies, which increase medium osmolality. While a negative effect on cell growth was observed with increasing osmolalities, adenovirus productivity was only affected for osmolalities higher than 430 mOsm.

  15. Interactions between Skeletal Muscle Myoblasts and their Extracellular Matrix Revealed by a Serum Free Culture System.

    Science.gov (United States)

    Chaturvedi, Vishal; Dye, Danielle E; Kinnear, Beverley F; van Kuppevelt, Toin H; Grounds, Miranda D; Coombe, Deirdre R

    2015-01-01

    Decellularisation of skeletal muscle provides a system to study the interactions of myoblasts with muscle extracellular matrix (ECM). This study describes the efficient decellularisation of quadriceps muscle with the retention of matrix components and the use of this matrix for myoblast proliferation and differentiation under serum free culture conditions. Three decellularisation approaches were examined; the most effective was phospholipase A2 treatment, which removed cellular material while maximizing the retention of ECM components. Decellularised muscle matrices were then solubilized and used as substrates for C2C12 mouse myoblast serum free cultures. The muscle matrix supported myoblast proliferation and differentiation equally as well as collagen and fibronectin. Immunofluorescence analyses revealed that myoblasts seeded on muscle matrix and fibronectin differentiated to form long, well-aligned myotubes, while myoblasts seeded on collagen were less organized. qPCR analyses showed a time dependent increase in genes involved in skeletal muscle differentiation and suggested that muscle-derived matrix may stimulate an increased rate of differentiation compared to collagen and fibronectin. Decellularized whole muscle three-dimensional scaffolds also supported cell adhesion and spreading, with myoblasts aligning along specific tracts of matrix proteins within the scaffolds. Thus, under serum free conditions, intact acellular muscle matrices provided cues to direct myoblast adhesion and migration. In addition, myoblasts were shown to rapidly secrete and organise their own matrix glycoproteins to create a localized ECM microenvironment. This serum free culture system has revealed that the correct muscle ECM facilitates more rapid cell organisation and differentiation than single matrix glycoprotein substrates.

  16. Changes in Serum Free Amino Acids and Muscle Fatigue Experienced during a Half-Ironman Triathlon.

    Directory of Open Access Journals (Sweden)

    Francisco Areces

    Full Text Available The aim of this study was to investigate the relationship between changes in serum free amino acids, muscle fatigue and exercise-induced muscle damage during a half-ironman triathlon. Twenty-six experienced triathletes (age = 37.0 ± 6.8 yr; experience = 7.4 ± 3.0 yr competed in a real half-ironman triathlon in which sector times and total race time were measured by means of chip timing. Before and after the race, a countermovement jump and a maximal isometric force test were performed, and blood samples were withdrawn to measure serum free amino acids concentrations, and serum creatine kinase levels as a blood marker of muscle damage. Total race time was 320 ± 37 min and jump height (-16.3 ± 15.2%, P 20%. However, neither the changes in serum free amino acids nor the tryptophan/BCAA ratio were related muscle fatigue or muscle damage during the race.

  17. Serum-Free Media and the Immunoregulatory Properties of Mesenchymal Stem Cells In Vivo and In Vitro

    Directory of Open Access Journals (Sweden)

    Mei Wu

    2014-02-01

    Full Text Available Background: Mesenchymal stem cells are capable of self-renewal and multi-lineage differentiation. They are used extensively to treat several diseases. Traditionally, mesenchymal stem cells are cultured in serum-containing media, typically supplemented with fetal bovine serum (FBS. However, the variability of FBS is likely to skew experimental results. Although serum-free media used to expand mesenchymal stem cells has facilitated remarkable achievements, immunomodulation of these cells in under serum-free conditions is poorly understood. We hypothesized that mesenchymal stem cells expanded in serum-free media will retain powerful immunoregulatory functions in vitro and in vivo. Design and Methods: Immunosuppressive activity and the immunomodulatory cytokines produced by mesenchymal stem cells in serum-free media were characterized in vitro. Immunomodulation by serum-free mesenchymal stem cell expansion in monocrotaline-induced pulmonary hypertension was explored in vivo. Results: Similar to cells in serum-containing media, mesenchymal stem cells expanded in serum-free media inhibited proliferation and apoptosis of CD4+T cells. They also exhibited strong immunosuppressive activities and secreted high levels of immunomodulatory cytokines such as PGE2, IDO1, COX2, IL-6, and IL-1β, but not HGF. On the other hand, growth of mesenchymal stem cells in serum-free media attenuated pulmonary vascular remodeling and inhibited mRNA expression of proinflammatory cytokines TNF-α, IFN-γ, IL-6, IL-1β, and IL-18. Conclusions: Mesenchymal stem cells in serum-free media maintained powerful immunomodulatory function in vitro and in vivo; serum-free media may replace serum-containing media for basic research and clinical applications.

  18. [Diagnostic significance of serum free DNA human telomerase reverse transcriptase quantitative determination on spinal cord injury].

    Science.gov (United States)

    Yang, M K; Tang, J; Xiang, Z; Zhang, X; Wang, J; Li, Z; Li, Y; Sheng, W B

    2018-02-06

    Objective: To investigate the relationship between the content of human telomerase reverse transcriptase (hTERT) and its clinical features in serum free DNA in patients with different degree of spinal cord injury. Methods: From December 2013 to December 2016, inpatients of the Central Hospital of Bazhong, Sichuan Province were enrolledand divided into the experimental group, the disease control group and the negative control group. For the experimental group: 46 patients with spinal cord injury were graded according to the criteria of the American Association of Spinal Cord Injury (ASIA), including 12 cases of grade A, 10 cases of grade B, 10 cases of grade C, 7 cases of grade D and 7 cases of grade E; for the disease control group: 15 patients with spinal fractures (without spinal cord injury) at the same period were included; and for the negative control group: 20 healthy adult volunteers aged 18-50 years were selected.Real-time fluorescence quantitative PCR and immunoblotting were performed to detect the content of hTERT in serum free DNA both in patients and healthy controls and to compare the difference between them. The results of the somatosensory evoked potential (SEP) of all patients were compared and analyzed.The receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of hTERT content in serum free DNA in patients with spinal cord injury. Results: Comparison of serum free DNA hTERT content: in the experimental group, the serum free DNA hTERT content of grade A, B, C, D, E was (99.63±8.23), (76.24±4.37), (46.07±5.43), (16.30±0.95) and (15.74±1.12)μg/L, respectively.While it was (15.01±1.39)μg/L in the disease control group and (14.54±1.03)μg/L in the negative control group. The total difference was statistically significant between patients of each group and the control group ( F =857.917, P spinal cord injury has a certain guiding significance for the diagnosis of spinal cord injury and the degree of injury.

  19. Active Stat3 is required for survival of human squamous cell carcinoma cells in serum-free conditions

    Directory of Open Access Journals (Sweden)

    DiGiovanni John

    2006-04-01

    Full Text Available Abstract Background Squamous cell carcinoma (SCC of the skin is the most aggressive form of non-melanoma skin cancer (NMSC, and is the single most commonly diagnosed cancer in the U.S., with over one million new cases reported each year. Recent studies have revealed an oncogenic role of activated signal transducer and activator of transcription 3 (Stat3 in many human tumors, especially in those of epithelial origin, including skin SCC. Stat3 is a mediator of numerous growth factor and cytokine signaling pathways, all of which activate it through phosphorylation of tyrosine 705. Results To further address the role of Stat3 in skin SCC tumorigenesis, we have analyzed a panel of human skin-derived cell lines ranging from normal human epidermal keratinocytes (NHEK, to non-tumorigenic transformed skin cells (HaCaT, to highly tumorigenic cells (SRB1-m7 and SRB12-p9 and observed a positive correlation between Stat3 phosphorylation and SCC malignancy. We next determined the role of Stat3 activity in cell proliferation and viability under serum-free culture conditions. This was accomplished by suppressing Stat3 activity in the SRB12-p9 cells through stable expression of a dominant negative acting form of Stat3β, which contains a tyrosine 705 to phenylalanine mutation (S3DN. The S3DN cells behaved similar to parental SRB12-p9 cells when cultured in optimal growth conditions, in the presence of 10% fetal calf serum. However, unlike the SRB12-p9 cells, S3DN cells underwent apoptotic cell death when cultured in serum-free medium (SFM. This was evidenced by multiple criteria, including accumulation of sub-G1 particles, induced PARP cleavage, and acquisition of the characteristic morphological changes associated with apoptosis. Conclusion This study provides direct evidence for a role for Stat3 in maintaining cell survival in the conditions of exogenous growth factor deprivation produced by culture in SFM. We also propose that delivery of the S3DN gene or

  20. Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method.

    Science.gov (United States)

    Kim, So-Jung; Jung, Ji-Won; Ha, Hye-Yeong; Koo, Soo Kyung; Kim, Eung-Gook; Kim, Jung-Hyun

    2017-03-01

    Embryonic stem cells (ESCs) can be expanded infinitely in vitro and have the potential to differentiate into hematopoietic stem cells (HSCs); thus, they are considered a useful source of cells for HSC production. Although several technical in vitro methods for engineering HSCs from pluripotent stem cells have been developed, clinical application of HSCs engineered from pluripotent stem cells is restricted because of the possibility of xenogeneic contamination resulting from the use of murine materials. Human ESCs (CHA-hES15) were cultured on growth factor-reduced Matrigel-coated dishes in the mTeSR1 serum-free medium. When the cells were 70% confluent, we initiated HSC differentiation by three methods involving (1) knockout serum replacement (KSR), cytokines, TGFb1, EPO, and FLT3L; (2) KSR, cytokines, and bFGF; or (3) cytokines and bFGF. Among the three differentiation methods, the minimal number of cytokines without KSR resulted in the greatest production of HSCs. The optimized method resulted in a higher proportion of CD34 + CD43 + hematopoietic progenitor cells (HPCs) and CD34 + CD45 + HPCs compared to the other methods. In addition, the HSCs showed the potential to differentiate into multiple lineages of hematopoietic cells in vitro . In this study, we optimized a two-step, serum-free, animal protein-free, KSR-free, feeder-free, chemically defined monolayer culture method for generation of HSCs and hematopoietic stem and progenitor cells (HSPCs) from human ESCs.

  1. Newly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours.

    Science.gov (United States)

    Bate-Eya, Laurel T; Ebus, Marli E; Koster, Jan; den Hartog, Ilona J M; Zwijnenburg, Danny A; Schild, Linda; van der Ploeg, Ida; Dolman, M Emmy M; Caron, Huib N; Versteeg, Rogier; Molenaar, Jan J

    2014-02-01

    Recently protocols have been devised for the culturing of cell lines from fresh tumours under serum-free conditions in defined neural stem cell medium. These cells, frequently called tumour initiating cells (TICs) closely retained characteristics of the tumours of origin. We report the isolation of eight newly-derived neuroblastoma TICs from six primary neuroblastoma tumours and two bone marrow metastases. The primary tumours from which these TICs were generated have previously been fully typed by whole genome sequencing (WGS). Array comparative genomic hybridisation (aCGH) analysis showed that TIC lines retained essential characteristics of the primary tumours and exhibited typical neuroblastoma chromosomal aberrations such as MYCN amplification, gain of chromosome 17q and deletion of 1p36. Protein analysis showed expression for neuroblastoma markers MYCN, NCAM, CHGA, DBH and TH while haematopoietic markers CD19 and CD11b were absent. We analysed the growth characteristics and confirmed tumour-forming potential using sphere-forming assays, subcutaneous and orthotopic injection of these cells into immune-compromised mice. Affymetrix mRNA expression profiling of TIC line xenografts showed an expression pattern more closely mimicking primary tumours compared to xenografts from classical cell lines. This establishes that these neuroblastoma TICs cultured under serum-free conditions are relevant and useful neuroblastoma tumour models. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Elevated serum free thyroxine by thyroxine analog radioimmunoassays in euthyroid patients with familial dysalbuminemic hyperthyroxinemia

    International Nuclear Information System (INIS)

    Rajatanavin, R.; Fournier, L.; DeCosimo, D.; Abreau, C.; Braverman, L.E.

    1982-01-01

    A study was done to ascertain whether the serum free T4 measured by free T4 radioimmunoassay kits would, like equilibrium dialysis, be normal in patients with familial dysalbuminemic hyperthyroxinemia. Five free T4 radioimmunoassay kits were used to measure free T4 in serum samples from 19 patients with familial dysalbuminemic hyperthyroxinemia and 20 healthy volunteers. Values (mean +/- SE) for T4, free T4 index, and free T4 (equilibrium dialysis) in these normal subjects and patients with familial dysalbuminemic hyperthyroxinemia, respectively, were as follows: T4, 8.1 +/- 0.2 and 18.3 +/- 0.7 μg/dL; free T4 index, 3.1 +/- 0.1 and 7.3 +/- 0.3 μg/dL; free T4, 1.4 +/- 0.1 and 1.2 +/- 0.1 ng/dL. The following free T4 radioimmunoassay methods were used: antibody coated microfine silica, microencapsulated antibody, two-step antibody-coated tube, and one-step 125 I-T4 analog (2 kits). The present findings in patients with familial dysalbuminemic hyperthyroxinemia and previous observations in ill euthyroid patients suggest that serum free T4 measured by some radioimmunoassay methods must be interpreted with caution in these two clinical situations

  3. Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21

    Directory of Open Access Journals (Sweden)

    Schneider Yves-Jacques

    2006-05-01

    Full Text Available Abstract Background The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debate regarding the value of Caco-2 cell culture to mimick in vivo situation. Indeed, some authors report in Caco-2 a low paracellular permeability and an ease of access of highly diffusible small molecules to the microvilli, due to an almost complete lack of mucus. The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. A co-culture system carried out in a serum-free medium and comprising both Caco-2 and HT29-5M21 cells was developed. The systematic use of a co-culture system requires the characterization of the monolayer under a given experimental procedure. Results In this study, we investigated the activity and localization of the alkaline phosphatase and the expression of IAP and MUC5AC genes to determine a correlation between these markers and the cellular composition of a differentiated monolayer obtained from a mixture of Caco-2 and HT29-5M21 cells. We observed that the culture conditions used (serum-free medium did not change the phenotype of each cell type, and produced a reproducible model. The alkaline phosphatase expression characterizing Caco-2 cells was influenced by the presence of HT29-5M21 cells. Conclusion The culture formed by 75% Caco-2 and 25% HT29-5M21 produce a monolayer containing the two main cell types of human intestinal epithelium and characterized by a reduced permeability to macromolecules.

  4. Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)

    Science.gov (United States)

    Nollevaux, Géraldine; Devillé, Christelle; El Moualij, Benaïssa; Zorzi, Willy; Deloyer, Patricia; Schneider, Yves-Jacques; Peulen, Olivier; Dandrifosse, Guy

    2006-01-01

    Background The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debate regarding the value of Caco-2 cell culture to mimick in vivo situation. Indeed, some authors report in Caco-2 a low paracellular permeability and an ease of access of highly diffusible small molecules to the microvilli, due to an almost complete lack of mucus. The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. A co-culture system carried out in a serum-free medium and comprising both Caco-2 and HT29-5M21 cells was developed. The systematic use of a co-culture system requires the characterization of the monolayer under a given experimental procedure. Results In this study, we investigated the activity and localization of the alkaline phosphatase and the expression of IAP and MUC5AC genes to determine a correlation between these markers and the cellular composition of a differentiated monolayer obtained from a mixture of Caco-2 and HT29-5M21 cells. We observed that the culture conditions used (serum-free medium) did not change the phenotype of each cell type, and produced a reproducible model. The alkaline phosphatase expression characterizing Caco-2 cells was influenced by the presence of HT29-5M21 cells. Conclusion The culture formed by 75% Caco-2 and 25% HT29-5M21 produce a monolayer containing the two main cell types of human intestinal epithelium and characterized by a reduced permeability to macromolecules. PMID:16670004

  5. Serum free-thyroxine modifications with age and in normal pregnancy

    International Nuclear Information System (INIS)

    Degrossi, O.J.; Altschuler, N.; Watanabe, T.; Pinkas, M.; Damilano, S.; Garcia del Rio, H.

    1982-01-01

    The possibility of using radioimmunoassay techniques (RIA) in the assessment of circulating thyroid hormones, total thyroxine (T4) and triiodothyronine (T3) has particularly increases the diagnosis of thyroid diseases. Thyroidal hormones circulate bound to proteins; therefore, variations in the transport capacity of the latter will produce important modifications in the T4 and T3 figures. Only small fractions, less than 0.05% for T4 and than 0.5% for T3, circulate in the free form and are considered as metabolically active forms of both hormones. In order to attain a correct clinical valuation, the rates of the free fractions as well as the total rates of these hormones must be known. Recently, these studies of free hormones are carried out by means of RIA, with the consequent advantages. The variations in serum free thyroxine (FT4) under certain physiogical conditions, such as for different ages and during pregnancy, were particularly studied

  6. In vitro maintenance of spermatogenesis in Xenopus laevis testis explants cultured in serum-free media

    International Nuclear Information System (INIS)

    Risley, M.S.; Miller, A.; Bumcrot, D.A.

    1987-01-01

    Spermatogenesis has been maintained for extended periods in Xenopus laevis testis explants cultured in serum-free media supplemented with bovine serum albumin, insulin, transferrin, follicle-stimulating hormone, dihydrotestosterone, testosterone, retinol, ascorbate, and tocopherol. The organization of the testis fragments was maintained for 28 days, and all stages of development were present throughout the culture period. 3 H-Thymidine-labeled secondary (Type B) spermatogonia developed in 28 days into spermatids at the acrosomal vesicle stage whereas labeled zygotene spermatocytes became mature spermatids in 28 days. Spermatogonial proliferation also continued in vitro for 28 days. Germ cell differentiation was not dependent upon exogenous testosterone, ascorbate, or tocopherol since 3 H-labeled spermatogonia became mature spermatids in testes cultured 35 days in media lacking these supplements. Autoradiography demonstrated that 55% of the luminal sperm present in explants cultured 10 days had differentiated in vitro. Sperm from testes cultured 10-35 days were similar to sperm from freshly dissected testes with regard to motility and fecundity, and eggs fertilized with sperm from explant cultures developed normally into swimming tadpoles. The results demonstrate the feasibility of maintaining vertebrate spermatogenesis in culture and suggest that in vitro analysis of Xenopus spermatogenesis using defined media may provide important insights into the evolution of regulatory mechanisms in spermatogenesis

  7. [The value of serum free light chain in differential diagnosis of monoclonal gammopathy of renal significance].

    Science.gov (United States)

    Li, C; Wen, Y B; Li, H; Su, W; Li, J; Cai, J F; Chen, L M; Li, X M; Li, X W

    2017-08-08

    Objective: To investigate the value of serum free light chain (FLC) in differential diagnosis of monoclonal gammopathy of renal significance (MGRS). Methods: Forty-nine hospitalized patients who underwent renal biopsy in Peking Union Medical College Hospital between January 2013 and December 2015 were included. Monoclonal gammopathy was detected by serum protein electrophoresis (SPE), serum immunofixation electrophoresis (IFE), urine IFE and serum FLC. All patients were classified as MGRS ( n =32) and monoclonal gammopathy of undetermined significance (MGUS) ( n =17). Results: Renal lesions in MGRS subgroup included light chain amyloidosis ( n =24, 75.0%), light chain deposition disease ( n =7, 21.9%), and fibrillary glomerulopathy ( n =1, 3.1%). Renal diseases in MGUS subgroup included membranous nephropathy ( n =10), focal segmental glomerulosclerosi (FSGS) ( n =3), diabetic glomerulopathy ( n =1), Henoch-Schonlein purpura nephritis ( n =1), anti-GBM disease concurrent with membranous nephropathy ( n =1) and glomerulomegaly ( n =1). Positive number of SPE, serum IFE, urine IFE and abnormal number of serum FLC ratio in MGRS subgroup were 12, 16, 23 and 30, respectively. Positive number of SPE, serum IFE, urine IFE and abnormal number of serum FLC ratio in MGUS subgroup were 11, 17, 6 and 3, respectively. MGRS and MGUS subgroups differed significantly in positive rate of serum IFE ( P value for MGRS, which was helpful for differential diagnosis of patients who had contraindication to renal biopsy.

  8. Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles

    Directory of Open Access Journals (Sweden)

    Jinghuan Li

    2015-05-01

    Full Text Available Extracellular vesicles (EVs play a significant role in cell–cell communication in numerous physiological processes and pathological conditions, and offer promise as novel biomarkers and therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that contribute to EV biogenesis and release from cells. However, little is known about how external stimuli such as cell culture conditions can affect the quantity and content of EVs. While N2a neuroblastoma cells cultured in serum-free (OptiMEM conditions did not result in EVs with significant biophysical or size differences compared with cells cultured in serum-containing (pre-spun conditions, the quantity of isolated EVs was greatly increased. Moreover, the expression levels of certain vesicular proteins (e.g. small GTPases, G-protein complexes, mRNA processing proteins and splicing factors, some of which were previously reported to be involved in EV biogenesis, were found to be differentially expressed in EVs under different culture conditions. These data, therefore, contribute to the understanding of how extracellular factors and intracellular molecular pathways affect the composition and release of EVs.

  9. Differentiating Graves' disease from subacute thyroiditis using ratio of serum free triiodothyronine to free thyroxine.

    Science.gov (United States)

    Sriphrapradang, Chutintorn; Bhasipol, Adikan

    2016-09-01

    The measurement of free thyroid hormone, instead of the total form, is more commonly used in current practice. We aimed to evaluate the usefulness of the ratio of serum free triiodothyronine (FT3, pg/mL) to free thyroxine (FT4, ng/dL) for differentiating Graves' disease from subacute thyroiditis. Medical records of thyrotoxic patients aged >15 years who had measurement of FT3, FT4 and thyrotropin on the first diagnosis of thyrotoxicosis before initiating treatment were retrospectively reviewed. Data were collected from all clinics, and were not limited to the endocrine clinic. Pregnant women were excluded. A total of 548 patients (468 with Graves' disease, 40 with subacute thyroiditis and 40 with toxic adenoma/multinodular goiter) were recruited. Mean age was 43.9 ± 15.4 years. Most were female 434 (79.2%), and goiter was present in 55.3%. Prevalence of T3-toxicosis and T4-toxicosis were 5.6% and 6.6%, respectively. Mean FT3/FT4 ratios were 4.62 ± 2 (10(-2) pg/ng) in patients with Graves' disease and 2.73 ± 0.5 in subacute thyroiditis. The area under the ROC curve of the FT3/FT4 ratio for diagnosis of Graves' disease was 0.83 (95%CI, 0.76-0.91). Cutoff level of this ratio >4.4 offered sensitivity of 47.2% and specificity of 92.8%. FT3/FT4 ratio of >4.4 (10(-2) pg/ng) may help in differentiating the cause of thyrotoxicosis.

  10. Increased serum free tryptophan in patients with diarrhea-predominant irritable bowel syndrome.

    Science.gov (United States)

    Christmas, David M; Badawy, Abdulla A-B; Hince, Dana; Davies, Simon J C; Probert, Christopher; Creed, Tom; Smithson, John; Afzal, Muhammad; Nutt, David J; Potokar, John P

    2010-10-01

    Irregularities of serotonin function in irritable bowel syndrome (IBS) may be due to changes in the metabolism of the serotonin precursor l-tryptophan. Dietary alteration of tryptophan intake may impact upon the mood and bowel symptoms of IBS. We hypothesized that diarrhea-predominant irritable bowel syndrome (d-IBS) patients would exhibit an increase in plasma tryptophan due to alterations in tryptophan metabolism. We also hypothesized that a diet low in tryptophan would reverse this change and reduce symptoms. Thirteen patients with d-IBS had fasting serum free and total tryptophan, large neutral amino acids, and 6 kynurenine metabolites measured before and after 2 weeks of a strict dairy-free diet. Baseline tryptophan parameters were compared with an age- and sex-matched control group. Changes in the specific tryptophan parameters before and after dairy-free diet were correlated with symptoms of IBS and mood. Compared with the control group, d-IBS patients at baseline exhibited significantly higher free serum tryptophan (10.5 ± 4.35 vs 4.75 ± 2.43 μmol/L [means ± standard deviation], P = .006) and significantly lower tryptophan dioxygenase and total tryptophan oxidation as measured by the kynurenine to free tryptophan and total kynurenines to free tryptophan ratios (23.37 ± 10.12 vs 55.33 ± 16.02, P < .001 and 49.34 ± 17.84 vs 258.46 ± 98.67, P < .001, respectively). Dairy-free diet did not modulate metabolites of the kynurenine pathway or symptoms. Tryptophan metabolism along the kynurenine pathway is inhibited in d-IBS, and a dairy-free diet does not alter this. Our findings are consistent with possible enhanced serotonin activity in d-IBS. Copyright © 2010 Elsevier Inc. All rights reserved.

  11. Shift of microRNA profile upon glioma cell migration using patient-derived spheroids and serum-free conditions

    DEFF Research Database (Denmark)

    Munthe, Sune; Halle, Bo; Boldt, Henning B

    2017-01-01

    Glioblastoma multiforme (GBM) is the most frequent malignant primary brain tumor. A major reason for the overall median survival being only 14.6 months is migrating tumor cells left behind after surgery. Another major reason is tumor cells having a so-called cancer stem cell phenotype being...... therefore resistant towards traditional chemo- and radiotherapy. A group of novel molecular targets are microRNAs (miRNAs). MiRNAs are small non-coding RNAs exerting post-transcriptional regulation of gene expression. The aim of this study was to identify differentially expressed miRNAs in migrating GBM...... cells using serum-free stem cell conditions. We used patient-derived GBM spheroid cultures for a novel serum-free migration assay. MiRNA expression of migrating tumor cells isolated at maximum migration speed was compared with corresponding spheroids using an OpenArray Real-Time PCR System. The mi...

  12. Serum-Free Media and the Immunoregulatory Properties of Mesenchymal Stem Cells In Vivo and In Vitro

    OpenAIRE

    Mei Wu; Zhi-Bo Han; Jun Feng Liu; You Wei Wang; Jian Zhong Zhang; Chun Tuan Li; Peng Liang Xin; Zhong Chao Han; Xiong Peng Zhu

    2014-01-01

    Background: Mesenchymal stem cells are capable of self-renewal and multi-lineage differentiation. They are used extensively to treat several diseases. Traditionally, mesenchymal stem cells are cultured in serum-containing media, typically supplemented with fetal bovine serum (FBS). However, the variability of FBS is likely to skew experimental results. Although serum-free media used to expand mesenchymal stem cells has facilitated remarkable achievements, immunomodulation of these cells in un...

  13. Fetal bovine serum and human constitutive androstane receptor: Evidence for activation of the SV23 splice variant by artemisinin, artemether, and arteether in a serum-free cell culture system

    Energy Technology Data Exchange (ETDEWEB)

    Lau, Aik Jiang; Chang, Thomas K.H., E-mail: thomas.chang@ubc.ca

    2014-06-01

    The naturally occurring SV23 splice variant of human constitutive androstane receptor (hCAR-SV23) is activated by di-(2-ethylhexyl)phthalate (DEHP), which is detected as a contaminant in fetal bovine serum (FBS). In our initial experiment, we compared the effect of dialyzed FBS, charcoal-stripped, dextran-treated FBS (CS-FBS), and regular FBS on the basal activity and ligand-activation of hCAR-SV23 in a cell-based reporter gene assay. In transfected HepG2 cells cultured in medium supplemented with 10% FBS, basal hCAR-SV23 activity varied with the type of FBS (regular > dialyzed > CS). DEHP increased hCAR-SV23 activity when 10% CS-FBS, but not regular FBS or dialyzed FBS, was used. With increasing concentrations (1–10%) of regular FBS or CS-FBS, hCAR-SV23 basal activity increased, whereas in DEHP-treated cells, hCAR-SV23 activity remained similar (regular FBS) or slightly increased (CS-FBS). Subsequent experiments identified a serum-free culture condition to detect DEHP activation of hCAR-SV23. Under this condition, artemisinin, artemether, and arteether increased hCAR-SV23 activity, whereas they decreased it in cells cultured in medium supplemented with 10% regular FBS. By comparison, FBS increased the basal activity of the wild-type isoform of hCAR (hCAR-WT), whereas it did not affect the basal activity of the SV24 splice variant (hCAR-SV24) or ligand activation of hCAR-SV24 and hCAR-WT by 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO). The use of serum-free culture condition was suitable for detecting CITCO activation of hCAR-WT and hCAR-SV24. In conclusion, FBS leads to erroneous classification of pharmacological ligands of hCAR-SV23 in cell-based assays, but investigations on functional ligands of hCAR isoforms can be conducted in serum-free culture condition. - Highlights: • FBS leads to erroneous pharmacological classification of hCAR-SV23 ligands. • Artemisinin, artemether, and arteether activate h

  14. [The prognostic value of baseline serum free light chain in cardiac amyloidosis].

    Science.gov (United States)

    Zhao, Lei; Tian, Zhuang; Fang, Quan

    2016-03-01

    To analyze the prognostic value of baseline serum free light chain (sFLC) in light-chain (AL) cardiac amyloidosis. Twenty-seven patients with AL cardiac amyloidosis were retrospectively reviewed from January 2014 to January 2015. sFLC was measured by immuoturbidimetric assay. Baseline characteristics, echocardiographic parameters and electrocardiogram data were analyzed. According to the median baseline dFLC (involved sFLC minus uninvolved sFLC), patients were categorized into either the low dFLC(≤ 307 mg/L) or the high dFLC group (>307 mg/L). More subjects in the high dFLC group with early/late diastolic mitral velocity ratio (E/A ratio) over 2 (71.4% vs 30.8%, P=0.035), and subjects in this group had a shorter median survival time than those in the low dFLC group (3 months vs 17 months, P=0.004). A similar phenomenon for median survival time was observed when the subjects were redivided either by a new cut-off value of 180 mg/L for dFLC (low dFLC group: 17 months; high dFLC group: 4 months, P=0.014) or a κ/λ ratio, in which subjects with κ type sFLC-ratio ≤ 19.6 and λ type sFLC-ratio>0.065 were in the low sFLC-ratio group (17 months) and those with κ type sFLC-ratio > 19.6 and λ type sFLC-ratio ≤ 0.065 were in the high sFLC-ratio group (4 months, P=0.023). In multivariate analysis, dFLC and New York Heart Association (NYHA)classification of cardiac function were two risk factors associated with all-cause mortality in patients, among which the hazard ratio for higher dFLC was 4.28 (95%CI 1.55-11.8, P=0.005). The level of sFLC could be a marker for the prognosis of AL cardiac amyloidosis.

  15. Interaction of estradiol and high density lipoproteins on proliferation of the human breast cancer cell line MCF-7 adapted to grow in serum free conditions

    International Nuclear Information System (INIS)

    Jozan, S.; Faye, J.C.; Tournier, J.F.; Tauber, J.P.; David, J.F.; Bayard, F.

    1985-01-01

    The responsiveness of the human mammary carcinoma cell line MCF-7 to estradiol and tamoxifen treatment has been studied in different culture conditions. Cells from exponentially growing cultures were compared with cells in their initial cycles after replating from confluent cultures (''confluent-log'' cells). It has been observed that estradiol stimulation of tritiated thymidine incorporation decreases with cell density and that ''confluent-log'' cells are estrogen unresponsive for a period of four cell cycles in serum-free medium conditions. On the other hand, growth of cells replated from exponentially growing, as well as from confluent cultures, can be inhibited by tamoxifen or a combined treatment with tamoxifen and the progestin levonorgestrel. This growth inhibitory effect can be rescued by estradiol when cells are replated from exponentially growing cultures. The growth inhibitory effect cannot be rescued by estradiol alone (10(-10) to 10(-8) M) when cells are replated from confluent cultures. In this condition, the addition of steroid depleted serum is necessary to reverse the state of estradiol unresponsiveness. Serum can be replaced by high density lipoproteins but not by low density lipoproteins or lipoprotein deficient serum. The present data show that estradiol and HDL interact in the control of MCF-7 cell proliferation

  16. Serum-free keloid fibroblast cell culture: an in vitro model for the study of aberrant wound healing.

    Science.gov (United States)

    Koch, R J; Goode, R L; Simpson, G T

    1997-04-01

    The purpose of this study was to develop an in vitro serum-free keloid fibroblast model. Keloid formation remains a problem for every surgeon. Prior evaluations of fibroblast characteristics in vitro, especially those of growth factor measurement, have been confounded by the presence of serum-containing tissue culture media. The serum itself contains growth factors, yet has been a "necessary evil" to sustain cell growth. The design of this study is laboratory-based and uses keloid fibroblasts obtained from five patients undergoing facial (ear lobule) keloid removal in a university-affiliated clinic. Keloid fibroblasts were established in primary cell culture and then propagated in a serum-free environment. The main outcome measures included sustained keloid fibroblast growth and viability, which was comparable to serum-based models. The keloid fibroblast cell cultures exhibited logarithmic growth, sustained a high cellular viability, maintained a monolayer, and displayed contact inhibition. Demonstrating model consistency, there was no statistically significant difference between the mean cell counts of the five keloid fibroblast cell lines at each experimental time point. The in vitro growth of keloid fibroblasts in a serum-free model has not been done previous to this study. The results of this study indicate that the proliferative characteristics described are comparable to those of serum-based models. The described model will facilitate the evaluation of potential wound healing modulators, and cellular effects and collagen modifications of laser resurfacing techniques, and may serve as a harvest source for contaminant-free fibroblast autoimplants. Perhaps its greatest utility will be in the evaluation of endogenous and exogenous growth factors.

  17. Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrick™ design, serum-free virus production and microcarrier-based cultivation of CV-1 cells.

    Science.gov (United States)

    Liu, Shuchang; Ruban, Ludmila; Wang, Yaohe; Zhou, Yuhong; Nesbeth, Darren N

    2017-02-01

    Vaccinia virus (VACV) is an established vector for vaccination and is beginning to prove effective as an oncolytic agent. Industrial production of VACV stands to benefit in future from advances made by synthetic biology in genome engineering and standardisation. The CV-1 cell line can be used for VACV propagation and has been used extensively with the CRISPR/Cas9 system for making precise edits of the VACV genome. Here we take first steps toward establishing a scalable synthetic biology platform for VACV production with CV-1 cells featuring standardised biological tools and serum free cell cultivation. We propose a new BioBrick™ plasmid backbone format for inserting transgenes into VACV. We then test the performance of CV-1 cells in propagation of a conventional recombinant Lister strain VACV, VACVL-15 RFP, in a serum-free process. CV-1 cells grown in 5% foetal bovine serum (FBS) Dulbecco's Modified Eagle Medium (DMEM) were adapted to growth in OptiPRO and VP-SFM brands of serum-free media. Specific growth rates of 0.047 h -1 and 0.044 h -1 were observed for cells adapted to OptiPRO and VP-SFM respectively, compared to 0.035 h -1 in 5% FBS DMEM. Cells adapted to OptiPRO and to 5% FBS DMEM achieved recovery ratios of over 96%, an indication of their robustness to cryopreservation. Cells adapted to VP-SFM showed a recovery ratio of 82%. Virus productivity in static culture, measured as plaque forming units (PFU) per propagator cell, was 75 PFU/cell for cells in 5% FBS DMEM. VP-SFM and OptiPRO adaptation increased VACV production to 150 PFU/cell and 350 PFU/cell respectively. Boosted PFU/cell from OptiPRO-adapted cells persisted when 5% FBS DMEM or OptiPRO medium was observed during the infection step and when titre was measured using cells adapted to 5% FBS DMEM or OptiPRO medium. Finally, OptiPRO-adapted CV-1 cells were successfully cultivated using Cytodex-1 microcarriers to inform future scale up studies.

  18. Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrick™ design, serum-free virus production and microcarrier-based cultivation of CV-1 cells

    Directory of Open Access Journals (Sweden)

    Shuchang Liu

    2017-02-01

    Full Text Available Vaccinia virus (VACV is an established vector for vaccination and is beginning to prove effective as an oncolytic agent. Industrial production of VACV stands to benefit in future from advances made by synthetic biology in genome engineering and standardisation. The CV-1 cell line can be used for VACV propagation and has been used extensively with the CRISPR/Cas9 system for making precise edits of the VACV genome. Here we take first steps toward establishing a scalable synthetic biology platform for VACV production with CV-1 cells featuring standardised biological tools and serum free cell cultivation. We propose a new BioBrick™ plasmid backbone format for inserting transgenes into VACV. We then test the performance of CV-1 cells in propagation of a conventional recombinant Lister strain VACV, VACVL-15 RFP, in a serum-free process. CV-1 cells grown in 5% foetal bovine serum (FBS Dulbecco’s Modified Eagle Medium (DMEM were adapted to growth in OptiPRO and VP-SFM brands of serum-free media. Specific growth rates of 0.047 h−1 and 0.044 h−1 were observed for cells adapted to OptiPRO and VP-SFM respectively, compared to 0.035 h−1 in 5% FBS DMEM. Cells adapted to OptiPRO and to 5% FBS DMEM achieved recovery ratios of over 96%, an indication of their robustness to cryopreservation. Cells adapted to VP-SFM showed a recovery ratio of 82%. Virus productivity in static culture, measured as plaque forming units (PFU per propagator cell, was 75 PFU/cell for cells in 5% FBS DMEM. VP-SFM and OptiPRO adaptation increased VACV production to 150 PFU/cell and 350 PFU/cell respectively. Boosted PFU/cell from OptiPRO-adapted cells persisted when 5% FBS DMEM or OptiPRO medium was observed during the infection step and when titre was measured using cells adapted to 5% FBS DMEM or OptiPRO medium. Finally, OptiPRO-adapted CV-1 cells were successfully cultivated using Cytodex-1 microcarriers to inform future scale up studies.

  19. Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII

    Science.gov (United States)

    2011-01-01

    Background Hemophilia A is a bleeding disorder caused by deficiency in coagulation factor VIII. Recombinant factor VIII (rFVIII) is an alternative to plasma-derived FVIII for the treatment of hemophilia A. However, commercial manufacturing of rFVIII products is inefficient and costly and is associated to high prices and product shortage, even in economically privileged countries. This situation may be solved by adopting more efficient production methods. Here, we evaluated the potential of transient transfection in producing rFVIII in serum-free suspension HEK 293 cell cultures and investigated the effects of different DNA concentration (0.4, 0.6 and 0.8 μg/106 cells) and repeated transfections done at 34° and 37°C. Results We observed a decrease in cell growth when high DNA concentrations were used, but no significant differences in transfection efficiency and in the biological activity of the rFVIII were noticed. The best condition for rFVIII production was obtained with repeated transfections at 34°C using 0.4 μg DNA/106 cells through which almost 50 IU of active rFVIII was produced six days post-transfection. Conclusion Serum-free suspension transient transfection is thus a viable option for high-yield-rFVIII production. Work is in progress to further optimize the process and validate its scalability. PMID:22115125

  20. Compound serum and hemin free medium for cultivation of ...

    African Journals Online (AJOL)

    Serum free cultivation of Leishmania is cost-effective and improves large scale production of well defined parasite material. Moreover, the production of recombinant pharmaceutical proteins requires cultivation of the host in a culture medium free of animal materials, so several culture media for Leishmania tarentolae ...

  1. Effect of retinol and cigarette-smoke and condensate on dye-coupled intercellular communication between hamster tracheal epithelial cells

    NARCIS (Netherlands)

    Rutten, A.A.J.J.L.; Jongen, W.M.F.; Haan, L.H.J.de; Hendriksen, E.G.J.; Koeman, J.H.

    1988-01-01

    The dye-coupled intercellular communication across gap junctions in primary hamster tracheal epithelial cells has been studied in serum-free, hormone-supplemented medium. In the absence of vitamin A, non-cytotoxic concentrations of cigarette-smoke condensate (CSC) inhibited intercellular

  2. Effects of all-trans retinol and cigarette smoke condensate on hamster tracheal epithelium in organ culture. I. A cell proliferation study

    NARCIS (Netherlands)

    Rutten, A.A.J.J.L.; Wilmer, J.W.G.M.; Beems, R.B.

    1988-01-01

    The effects of cigarette smoke condensate (CSC) and all-trans retinol on the cell proliferative activity of vitamin A-deprived hamster tracheal epithelium have been studied in vitamin A-deficient, serum-free, hormone-supplemented medium in organ culture. In the absence of retinol, CSC induced a

  3. Inactivated Enterovirus 71 Vaccine Produced by 200-L Scale Serum-Free Microcarrier Bioreactor System Provides Cross-Protective Efficacy in Human SCARB2 Transgenic Mouse.

    Science.gov (United States)

    Wu, Chia-Ying; Lin, Yi-Wen; Kuo, Chia-Ho; Liu, Wan-Hsin; Tai, Hsiu-Fen; Pan, Chien-Hung; Chen, Yung-Tsung; Hsiao, Pei-Wen; Chan, Chi-Hsien; Chang, Ching-Chuan; Liu, Chung-Cheng; Chow, Yen-Hung; Chen, Juine-Ruey

    2015-01-01

    Epidemics and outbreaks caused by infections of several subgenotypes of EV71 and other serotypes of coxsackie A viruses have raised serious public health concerns in the Asia-Pacific region. These concerns highlight the urgent need to develop a scalable manufacturing platform for producing an effective and sufficient quantity of vaccines against deadly enteroviruses. In this report, we present a platform for the large-scale production of a vaccine based on the inactivated EV71(E59-B4) virus. The viruses were produced in Vero cells in a 200 L bioreactor with serum-free medium, and the viral titer reached 10(7) TCID50/mL 10 days after infection when using an MOI of 10(-4). The EV71 virus particles were harvested and purified by sucrose density gradient centrifugation. Fractions containing viral particles were pooled based on ELISA and SDS-PAGE. TEM was used to characterize the morphologies of the viral particles. To evaluate the cross-protective efficacy of the EV71 vaccine, the pooled antigens were combined with squalene-based adjuvant (AddaVAX) or aluminum phosphate (AlPO4) and tested in human SCARB2 transgenic (Tg) mice. The Tg mice immunized with either the AddaVAX- or AlPO4-adjuvanted EV71 vaccine were fully protected from challenges by the subgenotype C2 and C4 viruses, and surviving animals did not show any degree of neurological paralysis symptoms or muscle damage. Vaccine treatments significantly reduced virus antigen presented in the central nervous system of Tg mice and alleviated the virus-associated inflammatory response. These results strongly suggest that this preparation results in an efficacious vaccine and that the microcarrier/bioreactor platform offers a superior alternative to the previously described roller-bottle system.

  4. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.

    Directory of Open Access Journals (Sweden)

    Akira Niwa

    Full Text Available Elucidating the in vitro differentiation of human embryonic stem (ES and induced pluripotent stem (iPS cells is important for understanding both normal and pathological hematopoietic development in vivo. For this purpose, a robust and simple hematopoietic differentiation system that can faithfully trace in vivo hematopoiesis is necessary. In this study, we established a novel serum-free monolayer culture that can trace the in vivo hematopoietic pathway from ES/iPS cells to functional definitive blood cells via mesodermal progenitors. Stepwise tuning of exogenous cytokine cocktails induced the hematopoietic mesodermal progenitors via primitive streak cells. These progenitors were then differentiated into various cell lineages depending on the hematopoietic cytokines present. Moreover, single cell deposition assay revealed that common bipotential hemoangiogenic progenitors were induced in our culture. Our system provides a new, robust, and simple method for investigating the mechanisms of mesodermal and hematopoietic differentiation.

  5. Clinical significance of two-step magnetic radioimmunoassay for determining serum free T3 and free T4

    International Nuclear Information System (INIS)

    Chen Bing

    1996-01-01

    The concentrations of the serum free T 3 (FT 3 ), free T 4 (FT 4 ), total triodothyronine (TT 3 ), total thyroxine (TT 4 ) and thyrotropin (TSH) are determined for 355 cases of normal persons, pregnant women and various thyropathetic patients. The normal values of FT 3 and FT 4 are 2.0-8.5 pmol/l, and 9.5-26.5 pmol/l, respectively. Neither FT 3 nor FT 4 is affected by the thyroxine combined with globulin (TBG), which is of unique diagnostic value for those with variable TBG (such as pregnant women hyperthyroidism, hypothyroidism, etc.), FT 3 and FT 4 are the most sensitive indices for diagnosis of hyperthyroidism, and hypothyroidism, respectively. In addition, FT 3 and FT 4 can greatly contribute to the observation of curative effectiveness under treatment

  6. Norepinephrine stimulates progesterone production in highly estrogenic bovine granulosa cells cultured under serum-free, chemically defined conditions

    Directory of Open Access Journals (Sweden)

    Piccinato Carla A

    2012-11-01

    Full Text Available Abstract Background Since noradrenergic innervation was described in the ovarian follicle, the actions of the intraovarian catecholaminergic system have been the focus of a variety of studies. We aimed to determine the gonadotropin-independent effects of the catecholamine norepinephrine (NE in the steroid hormone profile of a serum-free granulosa cell (GC culture system in the context of follicular development and dominance. Methods Primary bovine GCs were cultivated in a serum-free, chemically defined culture system supplemented with 0.1% polyvinyl alcohol. The culture features were assessed by hormone measurements and ultrastructural characteristics of GCs. Results GCs produced increasing amounts of estradiol and pregnenolone for 144h and maintained ultrastructural features of healthy steroidogenic cells. Progesterone production was also detected, although it significantly increased only after 96h of culture. There was a highly significant positive correlation between estradiol and pregnenolone production in high E2-producing cultures. The effects of NE were further evaluated in a dose–response study. The highest tested concentration of NE (10 (−7 M resulted in a significant increase in progesterone production, but not in estradiol or pregnenolone production. The specificity of NE effects on progesterone productio n was further investigated by incubating GCs with propranolol (10 (−8 M, a non-selective beta-adrenergic antagonist. Conclusions The present culture system represents a robust model to study the impact of intrafollicular factors, such as catecholamines, in ovarian steroidogenesis and follicular development. The results of noradrenergic effects in the steroidogenesis of GC have implications on physiological follicular fate and on certain pathological ovarian conditions such as cyst formation and anovulation.

  7. Norepinephrine stimulates progesterone production in highly estrogenic bovine granulosa cells cultured under serum-free, chemically defined conditions.

    Science.gov (United States)

    Piccinato, Carla A; Montrezor, Luis H; Collares, Cristhianna A V; Vireque, Alessandra A; Rosa e Silva, Alzira A M

    2012-11-22

    Since noradrenergic innervation was described in the ovarian follicle, the actions of the intraovarian catecholaminergic system have been the focus of a variety of studies. We aimed to determine the gonadotropin-independent effects of the catecholamine norepinephrine (NE) in the steroid hormone profile of a serum-free granulosa cell (GC) culture system in the context of follicular development and dominance. Primary bovine GCs were cultivated in a serum-free, chemically defined culture system supplemented with 0.1% polyvinyl alcohol. The culture features were assessed by hormone measurements and ultrastructural characteristics of GCs. GCs produced increasing amounts of estradiol and pregnenolone for 144h and maintained ultrastructural features of healthy steroidogenic cells. Progesterone production was also detected, although it significantly increased only after 96h of culture. There was a highly significant positive correlation between estradiol and pregnenolone production in high E2-producing cultures. The effects of NE were further evaluated in a dose-response study. The highest tested concentration of NE (10 (-7) M) resulted in a significant increase in progesterone production, but not in estradiol or pregnenolone production. The specificity of NE effects on progesterone production was further investigated by incubating GCs with propranolol (10 (-8) M), a non-selective beta-adrenergic antagonist. The present culture system represents a robust model to study the impact of intrafollicular factors, such as catecholamines, in ovarian steroidogenesis and follicular development. The results of noradrenergic effects in the steroidogenesis of GC have implications on physiological follicular fate and on certain pathological ovarian conditions such as cyst formation and anovulation.

  8. Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture

    International Nuclear Information System (INIS)

    Tunma, Somruthai; Song, Doo-Hoon; Kim, Si-Eun; Kim, Kyoung-Nam; Han, Jeon-Geon; Boonyawan, Dheerawan

    2013-01-01

    In this study, we focused on sericin hydrolysates, originating from silkworm used in serum-free human bone marrow-derived mesenchymal stem cells (hBM-MSCs) culture. We reported the effect of a covalent linkage between a bioactive protein molecule and polystyrene dish surface via a carbon intermediate layer which can slow down the release rate of protein compounds into the phosphate buffer saline (PBS) solution. Films of amorphous carbon (a-C) and functionalized-carbon were deposited on PS culture dish surfaces by using a DC magnetron sputtering system and RF PECVD system. We found that a-C based-films can increase the hydrophilicity and biocompatibility of polystyrene (PS) dishes, especially a-C films and a-C:N 2 films showed good attachment of hBM-MSCs at 24 h. However, in the case of silica surface (a-C:SiO x films), the cells showed a ragged and unattached boundary resulting from the presence of surface silanol groups. For the UV–vis absorbance, all carbon modified-PS dishes showed a lower release rate of sericin molecules into PBS solution than PS control. This revealed that the functionalized carbon could be enhanced by specific binding properties with given molecules. The carbon-coated PS dishes grafting with sericin protein were used in a serum-free condition. We also found that hBM-MSCs have higher percentage of proliferated cells at day 7 for the modified dishes with carbon films and coated with sericin than the PS control coated with sericin. The physical film properties were measured by atomic force microscopy (AFM), scanning electron microscope (SEM) and contact angle measurement. The presence of -NH 2 groups of sericin compounds on the PS dish was revealed by Fourier transform infrared spectroscopy (FTIR). The stability of covalent bonds of sericin molecules after washing out ungrafted sericin was confirmed by X-ray photoelectron spectroscopy (XPS).

  9. Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture

    Science.gov (United States)

    Tunma, Somruthai; Song, Doo-Hoon; Kim, Si-Eun; Kim, Kyoung-Nam; Han, Jeon-Geon; Boonyawan, Dheerawan

    2013-10-01

    In this study, we focused on sericin hydrolysates, originating from silkworm used in serum-free human bone marrow-derived mesenchymal stem cells (hBM-MSCs) culture. We reported the effect of a covalent linkage between a bioactive protein molecule and polystyrene dish surface via a carbon intermediate layer which can slow down the release rate of protein compounds into the phosphate buffer saline (PBS) solution. Films of amorphous carbon (a-C) and functionalized-carbon were deposited on PS culture dish surfaces by using a DC magnetron sputtering system and RF PECVD system. We found that a-C based-films can increase the hydrophilicity and biocompatibility of polystyrene (PS) dishes, especially a-C films and a-C:N2 films showed good attachment of hBM-MSCs at 24 h. However, in the case of silica surface (a-C:SiOx films), the cells showed a ragged and unattached boundary resulting from the presence of surface silanol groups. For the UV-vis absorbance, all carbon modified-PS dishes showed a lower release rate of sericin molecules into PBS solution than PS control. This revealed that the functionalized carbon could be enhanced by specific binding properties with given molecules. The carbon-coated PS dishes grafting with sericin protein were used in a serum-free condition. We also found that hBM-MSCs have higher percentage of proliferated cells at day 7 for the modified dishes with carbon films and coated with sericin than the PS control coated with sericin. The physical film properties were measured by atomic force microscopy (AFM), scanning electron microscope (SEM) and contact angle measurement. The presence of sbnd NH2 groups of sericin compounds on the PS dish was revealed by Fourier transform infrared spectroscopy (FTIR). The stability of covalent bonds of sericin molecules after washing out ungrafted sericin was confirmed by X-ray photoelectron spectroscopy (XPS).

  10. Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture

    Energy Technology Data Exchange (ETDEWEB)

    Tunma, Somruthai [The Graduate School, Chiang Mai University, 239 Huay Kaew Road, Muang District, Chiang Mai 50200 (Thailand); Thailand Center of Excellence in Physics (ThEP), 239 Huay Kaew Road, Muang District, Chiang Mai 50200 (Thailand); Song, Doo-Hoon [Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752 (Korea, Republic of); Kim, Si-Eun; Kim, Kyoung-Nam [Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752 (Korea, Republic of); Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752 (Korea, Republic of); Han, Jeon-Geon [Center for Advanced Plasma Surface Technology, Sungkyunkwan University, 300 Chunchun-dong, Jangan-gu, Suwon 440-746 (Korea, Republic of); Boonyawan, Dheerawan [Thailand Center of Excellence in Physics (ThEP), 239 Huay Kaew Road, Muang District, Chiang Mai 50200 (Thailand); Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, 239 Huay Kaew Road, Muang District, Chiang Mai 50200 (Thailand)

    2013-10-15

    In this study, we focused on sericin hydrolysates, originating from silkworm used in serum-free human bone marrow-derived mesenchymal stem cells (hBM-MSCs) culture. We reported the effect of a covalent linkage between a bioactive protein molecule and polystyrene dish surface via a carbon intermediate layer which can slow down the release rate of protein compounds into the phosphate buffer saline (PBS) solution. Films of amorphous carbon (a-C) and functionalized-carbon were deposited on PS culture dish surfaces by using a DC magnetron sputtering system and RF PECVD system. We found that a-C based-films can increase the hydrophilicity and biocompatibility of polystyrene (PS) dishes, especially a-C films and a-C:N{sub 2} films showed good attachment of hBM-MSCs at 24 h. However, in the case of silica surface (a-C:SiO{sub x} films), the cells showed a ragged and unattached boundary resulting from the presence of surface silanol groups. For the UV–vis absorbance, all carbon modified-PS dishes showed a lower release rate of sericin molecules into PBS solution than PS control. This revealed that the functionalized carbon could be enhanced by specific binding properties with given molecules. The carbon-coated PS dishes grafting with sericin protein were used in a serum-free condition. We also found that hBM-MSCs have higher percentage of proliferated cells at day 7 for the modified dishes with carbon films and coated with sericin than the PS control coated with sericin. The physical film properties were measured by atomic force microscopy (AFM), scanning electron microscope (SEM) and contact angle measurement. The presence of -NH{sub 2} groups of sericin compounds on the PS dish was revealed by Fourier transform infrared spectroscopy (FTIR). The stability of covalent bonds of sericin molecules after washing out ungrafted sericin was confirmed by X-ray photoelectron spectroscopy (XPS).

  11. Thyroid Hormone Supplementation Restores Spatial Memory, Hippocampal Markers of Neuroinflammation, Plasticity-Related Signaling Molecules, and β-Amyloid Peptide Load in Hypothyroid Rats.

    Science.gov (United States)

    Chaalal, Amina; Poirier, Roseline; Blum, David; Laroche, Serge; Enderlin, Valérie

    2018-05-23

    Hypothyroidism is a condition that becomes more prevalent with age. Patients with untreated hypothyroidism have consistently reported symptoms of severe cognitive impairments. In patients suffering hypothyroidism, thyroid hormone supplementation offers the prospect to alleviate the cognitive consequences of hypothyroidism; however, the therapeutic value of TH supplementation remains at present uncertain and the link between cellular modifications associated with hypothyroidism and neurodegeneration remains to be elucidated. In the present study, we therefore evaluated the molecular and behavioral consequences of T3 hormone replacement in an animal model of hypothyroidism. We have previously reported that the antithyroid molecule propylthiouracil (PTU) given in the drinking water favors cerebral atrophy, brain neuroinflammation, Aβ production, Tau hyperphosphorylation, and altered plasticity-related cell-signaling pathways in the hippocampus in association with hippocampal-dependent spatial memory deficits. In the present study, our aim was to explore, in this model, the effect of hippocampal T3 signaling normalization on various molecular mechanisms involved in learning and memory that goes awry under conditions of hypothyroidism and to evaluate its potential for recovery of hippocampal-dependent memory deficits. We report that T3 supplementation can alleviate hippocampal-dependent memory impairments displayed by hypothyroid rats and normalize key markers of thyroid status in the hippocampus, of neuroinflammation, Aβ production, and of cell-signaling pathways known to be involved in synaptic plasticity and memory function. Together, these findings suggest that normalization of hippocampal T3 signaling is sufficient to reverse molecular and cognitive dysfunctions associated with hypothyroidism.

  12. Enhancement of Human Prolactin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture

    Directory of Open Access Journals (Sweden)

    Herbert Rodrigues Goulart

    2010-01-01

    Full Text Available Sodium butyrate (NaBu has been used as a productivity enhancer for the synthesis of recombinant proteins in Chinese hamster ovary (CHO cells. Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL from CHO cells was investigated for the first time. CHO cell cultures were submitted to a treatment with different concentrations of NaBu (0.25 to 4 mM. Quantitative and qualitative analyses by reverse-phase high-performance liquid chromatography (RP-HPLC and Western blot or SDS-PAGE, carried out directly on CHO-conditioned medium, showed that the highest hPRL expression was obtained with 1 mM NaBu. In vitro biological assays based on noble rat lymphoma (Nb2 and mouse pro-B lymphoma (Ba/F3-LLP cells were carried out on purified hPRL. Its bioactivity in the presence of NaBu was not apparently different from that of the First International Reference Reagent of recombinant hPRL (WHO 97/714. Our results show that NaBu increased the synthesis of recombinant hPRL in CHO cells, apparently without compromising either its structure or function.

  13. Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model.

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    Rebecca Lamb

    Full Text Available Primary human epidermal stem cells isolated from skin tissues and subsequently expanded in tissue culture are used for human therapeutic use to reconstitute skin on patients and to generate artificial skin in culture for academic and commercial research. Classically, epidermal cells, known as keratinocytes, required fibroblast feeder support and serum-containing media for serial propagation. In alignment with global efforts to remove potential animal contaminants, many serum-free, feeder-free culture methods have been developed that support derivation and growth of these cells in 2-dimensional culture. Here we show that keratinocytes grown continually in serum-free and feeder-free conditions were unable to form into a stratified, mature epidermis in a skin equivalent model. This is not due to loss of cell potential as keratinocytes propagated in serum-free, feeder-free conditions retain their ability to form stratified epidermis when re-introduced to classic serum-containing media. Extracellular calcium supplementation failed to improve epidermis development. In contrast, the addition of serum to commercial, growth media developed for serum-free expansion of keratinocytes facilitated 3-dimensional stratification in our skin equivalent model. Moreover, the addition of heat-inactivated serum improved the epidermis structure and thickness, suggesting that serum contains factors that both aid and inhibit stratification.

  14. IgD multiple myeloma: Clinical, biological features and prognostic value of the serum free light chain assay.

    Science.gov (United States)

    Djidjik, R; Lounici, Y; Chergeulaïne, K; Berkouk, Y; Mouhoub, S; Chaib, S; Belhani, M; Ghaffor, M

    2015-09-01

    IgD multiple myeloma (MM) is a rare subtype of myeloma, it affects less than 2% of patients with MM. To evaluate the clinical and prognostic attributes of serum free light chains (sFLCs) analysis, we examined 17 cases of IgD MM. From 1998 to 2012, we obtained 1250 monoclonal gammapathies including 590 multiple myeloma and 17 patients had IgD MM. With preponderance of men patients with a mean age at diagnosis of: 59±12years. Patients with IgD MM have a short survival (Median survival=9months). The presenting features included: bone pain (75%), lymphadenopathy (16%), hepatomegaly (25%), splenomegaly (8%), associated AL amyloidosis (6%), renal impairment function (82%), infections (47%), hypercalcemia (37%) and anemia (93%). Serum electrophoresis showed a subtle M-spike (Mean=13.22±10g/L) in all patients associated to a hypogammaglobulinemia. There was an over-representation of Lambda light chain (65%); high serum β2-microglobulin in 91% and Bence Jones proteinuria was identified in 71%. The median rate of sFLCs κ was 19.05mg/L and 296.75mg/L for sFLCs λ. sFLCR was abnormal in 93% of patients and it showed concordance between baseline sFLCR and the survival (P=0.034). The contribution of FLC assay is crucial for the prognosis of patients with IgD MM. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  15. Prognostic value of the serum free light chain ratio in newly diagnosed myeloma: proposed incorporation into the international staging system.

    Science.gov (United States)

    Snozek, C L H; Katzmann, J A; Kyle, R A; Dispenzieri, A; Larson, D R; Therneau, T M; Melton, L J; Kumar, S; Greipp, P R; Clark, R J; Rajkumar, S V

    2008-10-01

    To determine if the serum free light chain (FLC) ratio has prognostic value in patients with symptomatic multiple myeloma (MM), baseline serum samples from a well-characterized cohort of 790 newly diagnosed MM patients were tested with the FLC assay. FLC ratio was calculated as kappa/lambda (reference range 0.26-1.65). On the basis of the distribution of values, a cutpoint kappa/lambda FLC ratio of 32 was chosen for further analysis. Overall survival was significantly inferior in patients with an abnormal FLC ratio of 32 (n=479) compared with those with an FLC ratio between 0.03 and 32 (n=311), with median survival of 30 versus 39 months, respectively. We incorporated abnormal FLC ratio with the International Staging System (ISS) risk factors (that is, albumin or=3.5 g/l), to create a risk stratification model with improved prognostic capabilities. Patients with 0, 1, 2 or 3 adverse risk factors had significantly different overall survival, with median survival times of 51, 39, 30 and 22 months, respectively (P<0.001). These findings suggest that the serum FLC ratio at initial diagnosis is an important predictor of prognosis in myeloma, and can be incorporated into the ISS for improved risk stratification.

  16. Serum Free Light Chains

    Science.gov (United States)

    ... lab's website in order to provide you with background information about the test(s) you had performed. You will need to return ... the free light chains have a much shorter half-life (3-5 hours) than ... cell disorders, the test is also used for assessing response and minimal ...

  17. Clinical usefulness of serum free light chains measurement in patients with multiple myeloma: comparative analysis of two different tests

    Directory of Open Access Journals (Sweden)

    Tadeusz Kubicki

    2017-01-01

    Full Text Available Introduction: There are two commercially available tests for measurement of serum free light chains (sFLC in multiple myeloma (MM patients – Freelite and N Latex FLC. The aim of this study was to perform an assessment and direct comparison of the usefulness of the methods in routine clinical practice.Methods: 40 refractory/relapsed MM patients underwent routine disease activity assessment studies, along with sFLC analysis using both assays. Correlation and concordance between the tests and sensitivity of studied methods of sFLC assessment were established. Special attention was focused on sFLC results in patients finally evaluated after completing the treatment. Results: A weak correlation for the measurement of both κ [Passing–Bablok slope (PB = 0.7681] and λ chains [(PB = 1.542] was found. Using Bland–Altman plots, a bias of 0.0467 (κ and -0.2133 (λ between the measurements was documented. The concordance coefficient equaled 0.87 for κ, 0.62 for λ and 0.52 for κ/λ ratio. Ten patients had an abnormal Freelite assay κ/λ ratio and normal N Latex FLC κ/λ ratio. Three of these patients had negative serum protein electrophoresis results and fulfilled diagnostic criteria of stringent complete remission (sCR according to N Latex FLC (but not according to Freelite. When the κ/λ ratio obtained by both methods was compared to patients’ serum/urine protein electrophoresis and immunofixation results, sensitivity of Freelite and N Latex FLC was established to be 62.5% and 41%, respectively. Conclusions: There was no strong correlation or concordance between the two assays, and the sensitivity in terms of sFLC detection was different. This may cause problems when diagnosis of sCR is considered.

  18. Determination of serum free thyroxine concentration (FT4) by means of fT4-fraction and total thyroxine concentration

    International Nuclear Information System (INIS)

    Passath, A.; Leb, G.

    1985-01-01

    A new equilibrium assay for the determination of serum free thyroxine was evaluated in 514 patients. The assay comprises a two-vial-procedure to measure total thyroxine and free thyroxine fraction by use of monoclonal antibodies. Free thyroxine concentrations are calculated from fT 4 -fraction and total thyroxine concentration readings. In euthyroidism the average free thyroxine fraction (%fT 4 ) was 0.011%, in hyperthyroidism this fraction was elevated, in hypothyroidism it was below normal. In patients with TBG anomalies, TBG values were inversely correlated with fT 4 fraction readings. The 'euthyroid reference range' of FT 4 (SPAC ET) was between 0.70 to 1.78ng/dl. This euthyroid range of FT 4 was determined from TT 4 concentrations measured by T 4 -RIA (SPAC T 4 MONO) which were 30% above TT 4 values measured by conventional T 4 -RIA (SPAC T 4 POLY; polyclonal antibodies). However, a different euthyroid range of FT 4 between 0.55 to 1.30 ng/dl was observed as well as by other investigators when conventional T 4 -RIA measurements were used for calculation of FT 4 values. Our results indicate that calculated FT 4 concentration values are highly dependent on the methods used for determination of total thyroxine concentrations. Precision and reproducability of this two vial equilibrium assay did not meet the requirements mandatory for the application as a clinical routine diagnostic procedure, and its general use for this purpose can as yet not be recommended. (Author)

  19. The comparison of free androgen index and serum free testosterone levels in women with hirsutism or polycystic ovary syndrome

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    M. Metin Yıldırımkaya

    2011-06-01

    Full Text Available In many laboratories free testosterone can not be measured, so that free androgen index is suggested instead. The aim of this study was to compare free androgen index and serum free testosterone levels measured by radioimmunoassay in women with hirsutism or polycystic ovary syndrome.Materials and methods: Totally 94 women referred to the polyclinics of Ankara Numune Hospital were retrospectively included. Three patient groups were composed; 55 of hirsutism, 20 of polycystic ovary syndrome and 19 of both hirsutism and polycystic ovary syndrome. Total testosterone and sex hormone binding globuline levels were measured by chemiluminescence method and free testosterone levels were measured by radioimmunoassay. Free androgen index was calculated from total testosterone and sex hormone binding globuline.Results: There was a significant positive correlation between free testosterone and free androgen index in patients with hirsutism, in patients with polycystic ovary syndrome, in patients with hirsutism and polycystic ovary syndrome, and in total patient group [r(hirsutism=0,597, r(PCOS=0,617, r(hirsutism and PCOS=0,779, r(total patient group=0,649, P<0,01].Receiver operating characteristics curves were drawn to assess the diagnostic power of parameters for all patient groups [For hirsutism (n=55 auROC (FT=0,431 auROC (FAI=0,485] [For PCOS (n=20 auROC (FT=0,431 auROC (FAI=0,359] [For hirsutism and PCOS (n=19 auROC (FT=0,676 auROC (FAI=0,669]. In our study, free testosterone and free androgen index were found useful to diagnose ‘hirsutism and polycystic ovary syndrome’ but not others.Conclusion: Free androgen index can be used instead of free testosterone in hirsutism and polycystic ovary syndrome for diagnosis. J Clin Exp Invest 2011;2(2:152-6

  20. Effects of age and health on the euthyroid reference ranges for serum free thyroxine and free triiodothyronine

    International Nuclear Information System (INIS)

    Midgley, J.E.M.

    1985-01-01

    Age-related trends in serum free thyroxine (FT 4 ) and free triiodothyronine (FT 3 ) concentrations were measured in 7248 euthyroid subjects (age-range 3 months to 106 years). 5700 were patients referred to hospitals for investigation of suspected thyroid dysfunction, but who were diagnosed euthyroid. 1548 were healthy blood donors (age-range 18-63 years) with no indication of thyroid dysfunction. FT 4 concentrations were little affected by the age, the sex or the state of health of the subjects in either group. Serum FT 3 concentrations were significantly affected by both age and health factors. The upper limit of the euthyroid reference range for young subjects up to 15 years was about 20% higher (10.4 pmol/l) than for adult subjects older than 25 years (8.8 pmol/l). The change in the upper limits typical of young subjects to that typical of adults occurred steadily over the decade 15-25 years. After this age, little further change occurred, especially in healthy subjects. Additionally, the lower limit of the euthyroid range for FT 3 was extended by the inclusion in the reference group of patients referred to hospitals. Compared with the lower limit of the FT 3 range for healthy subjects (5 pmol/l), the corresponding limit for referred subjects (young or adult) was 3.5-3.8 pmol/l. Broadening of the FT 3 reference range was probably brought about by a significant number of patients in the hospital-referred group with the 'low-T 3 syndrome' of mild non-thyroidal illness. Accordingly, FT 3 was inferior to FT 4 in the discrimination of hypothyroidism, as FT 4 was unaffected by this phenomenon. Effects of age and non-thyroidal illness on serum FT 3 concentrations require great care when selecting subjects for a laboratory euthyroid reference range typical of the routine workload. Constraints on the choice of subjects for FT 4 reference ranges are less stringent. (orig.) [de

  1. Ratio of serum free triiodothyronine to free thyroxine in Graves' hyperthyroidism and thyrotoxicosis caused by painless thyroiditis.

    Science.gov (United States)

    Yoshimura Noh, Jaeduk; Momotani, Naoko; Fukada, Shuji; Ito, Koichi; Miyauchi, Akira; Amino, Nobuyuki

    2005-10-01

    The serum T3 to T4 ratio is a useful indicator for differentiating destruction-induced thyrotoxicosis from Graves' thyrotoxicosis. However, the usefulness of the serum free T3 (FT3) to free T4 (FT4) ratio is controversial. We therefore systematically evaluated the usefulness of this ratio, based on measurements made using two widely available commercial kits in two hospitals. Eighty-two untreated patients with thyrotoxicosis (48 patients with Graves' disease and 34 patients with painless thyroiditis) were examined in Kuma Hospital, and 218 patients (126 with Graves' disease and 92 with painless thyroiditis) and 66 normal controls were examined in Ito Hospital. The FT3 and FT4 values, as well as the FT3/FT4 ratios, were significantly higher in the patients with Graves' disease than in those with painless thyroiditis in both hospitals, but considerable overlap between the two disorders was observed. Receiver operating characteristic (ROC) curves for the FT3 and FT4 values and the FT3/FT4 ratios of patients with Graves' disease and those with painless thyroiditis seen in both hospitals were prepared, and the area under the curves (AUC), the cut-off points for discriminating Graves' disease from painless thyroiditis, the sensitivity, and the specificity were calculated. AUC and sensitivity of the FT(3)/FT(4) ratio were smaller than those of FT(3) and FT(4) in both hospitals. The patients treated at Ito hospital were then divided into 4 groups according to their FT4 levels (A: 2.3 approximately 5.4 ng/dl), and the AUC, cut-off points, sensitivity, and specificity of the FT(3)/FT(4) ratios were calculated. The AUC and sensitivity of each group increased with the FT4 levels (AUC: 57.8%, 72.1%, 91.1%, and 93.4%, respectively; sensitivity: 62.6%, 50.0%, 77.8%, and 97.0%, respectively). The means +/- SE of the FT3/FT4 ratio in the Graves' disease groups were 3.1 +/- 0.22, 3.1 +/- 0.09, 3.2 +/- 0.06, and 3.1 +/- 0.07, respectively, versus 2.9 +/- 0.1, 2.6 +/- 0.07, 2.5 +/- 0

  2. Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system

    Directory of Open Access Journals (Sweden)

    Cardoso Tereza C

    2012-05-01

    Full Text Available Abstract Background The possibility for isolating bovine mesenchymal multipotent cells (MSCs from fetal adnexa is an interesting prospect because of the potential for these cells to be used for biotechnological applications. Bone marrow and adipose tissue are the most common sources of MSCs derived from adult animals. However, little knowledge exists about the characteristics of these progenitors cells in the bovine species. Traditionally most cell cultures are developed in two dimensional (2D environments. In mammalian tissue, cells connect not only to each other, but also support structures called the extracellular matrix (ECM. The three-dimensional (3D cultures may play a potential role in cell biotechnology, especially in tissue therapy. In this study, bovine-derived umbilical cord Wharton’s jelly (UC-WJ cells were isolated, characterized and maintained under 3D-free serum condition as an alternative of stem cell source for future cell banking. Results Bovine-derived UC-WJ cells, collected individually from 5 different umbilical cords sources, were successfully cultured under serum-free conditions and were capable to support 60 consecutive passages using commercial Stemline® mesenchymal stem cells expansion medium. Moreover, the UC-WJ cells were differentiated into osteocytes, chondrocytes, adipocytes and neural-like cells and cultured separately. Additionally, the genes that are considered important embryonic, POU5F1 and ITSN1, and mesenchymal cell markers, CD105+, CD29+, CD73+ and CD90+ in MSCs were also expressed in five bovine-derived UC-WJ cultures. Morphology of proliferating cells typically appeared fibroblast-like spindle shape presenting the same viability and number. These characteristics were not affected during passages. There were 60 chromosomes at the metaphase, with acrocentric morphology and intense telomerase activity. Moreover, the proliferative capacity of T cells in response to a mitogen stimulus was suppressed when

  3. In vitro culture of human osteosarcoma cell lines: a comparison of functional characteristics for cell lines cultured in medium without and with fetal calf serum.

    Science.gov (United States)

    Bruserud, Oystein; Tronstad, Karl Johan; Berge, Rolf

    2005-06-01

    Experimental in vitro models including well-characterised cell lines can be used to identify possible new therapeutic targets for the treatment of osteosarcoma. Culture media including inactivated serum is often recommended for in vitro culture of osteosarcoma cells, but the serum component then represents a nonstandardised parameter including a wide range of unidentified mediators. To improve the standardisation we have investigated whether serum-free culture media can be used in experimental in vitro studies of osteosarcoma cell lines. The seven osteosarcoma cell lines Cal72, SJSA-1, Saos-2, SK-ES-1, U2OS, 143.98.2, and KHOS-32IH were cultured in vitro in various serum-free media and media supplemented with 10% heat-inactivated fetal calf serum (FCS). Although proliferation often was relatively low in serum-free media (X-vivo 10, X-vivo 15, X-vivo 20, Stem Span SFEM), some cell lines (Cal72, KHOS-32IH, Saos-2) showed proliferation comparable with the recommended FCS-containing media even when using serum-free conditions. The optimal serum-free medium then varied between cell lines. We also compared 6 different FCS-containing media (including Stem Span with 10% FCS) and the optimal FCS-containing medium varied between cell lines. However, all cell lines proliferated well in Stem Span with FCS, and this medium was regarded as optimal for four of the lines. FCS could not be replaced by fatty acids or low density lipoprotein when testing the Stem Span medium. The release of a wide range of soluble mediators showed only minor differences when using serum-free and FCS-containing media (including Stem Span with and without FCS), and serum-free Stem Span could also be used for in vitro studies of mitogen-stimulated T cell activation in the presence of accessory osteosarcoma cells. The use of Stem Span with 10% FCS allowed the release of a wide range of chemokines by osteosarcoma cell lines (Cal72, SJSA-1), and the chemokine release profile was very similar to the

  4. Analytical Criticalities Associated to Different Immunological Methods for Serum Free Light Chain Detection in Plasma Cell Dyscrasias: A Description of Particular Clinical Cases

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    Rocco Sabatino

    2017-04-01

    Full Text Available Current criteria for differential diagnosis of multiple myeloma (MM, Monoclonal gammopathy of undetermined significance (MGUS, and smoldering multiple myeloma (SMM are included in the 2003 guidelines by the International Myeloma Working Group (IMWG. An updated version was then published in 2014, highlighting the importance of serum free light chain (sFLC detection, as well as the κ/λ ratio as excellent indicators of clonality. At present, two commercial assays for sFLC quantification are available: the Freelite™ assay and the N-Latex assay. The first was developed by The Binding Site based on a mixture of polyclonal antibodies directed against a variety of FLC epitopes. It may be run on a wide range of nephelometers, as well as on turbidimeters. The second method was developed by Siemens and runs exclusively on Siemens instruments. It employs a probe mixture of mouse monoclonal antibodies. The aim of our study was to evaluate sFLC measurement and calculated κ/λ ratio in 85 patients with monoclonal gammopathies (MGs in order to compare methods. We demonstrated that there is only a moderate concordance between the two FLC assays. In particular, in one case, we observed no qualitative alterations of the serum protein pattern, and in the absence of a Freelite™ assay, sFLC measurement would not have been possible to highlight the increase of λ FLC.

  5. Stepwise Differentiation of Pluripotent Stem Cells into Osteoblasts Using Four Small Molecules under Serum-free and Feeder-free Conditions

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    Kosuke Kanke

    2014-06-01

    Full Text Available Pluripotent stem cells are a promising tool for mechanistic studies of tissue development, drug screening, and cell-based therapies. Here, we report an effective and mass-producing strategy for the stepwise differentiation of mouse embryonic stem cells (mESCs and mouse and human induced pluripotent stem cells (miPSCs and hiPSCs, respectively into osteoblasts using four small molecules (CHIR99021 [CHIR], cyclopamine [Cyc], smoothened agonist [SAG], and a helioxanthin-derivative 4-(4-methoxyphenylpyrido[4′,3′:4,5]thieno[2,3-b]pyridine-2-carboxamide [TH] under serum-free and feeder-free conditions. The strategy, which consists of mesoderm induction, osteoblast induction, and osteoblast maturation phases, significantly induced expressions of osteoblast-related genes and proteins in mESCs, miPSCs, and hiPSCs. In addition, when mESCs defective in runt-related transcription factor 2 (Runx2, a master regulator of osteogenesis, were cultured by the strategy, they molecularly recapitulated osteoblast phenotypes of Runx2 null mice. The present strategy will be a platform for biological and pathological studies of osteoblast development, screening of bone-augmentation drugs, and skeletal regeneration.

  6. Specific estrogen-induced cell proliferation of cultured Syrian hamster renal proximal tubular cells in serum-free chemically defined media

    International Nuclear Information System (INIS)

    Oberley, T.D.; Lauchner, L.J.; Pugh, T.D.; Gonzalez, A.; Goldfarb, S.; Li, S.A.; Li, J.J.

    1989-01-01

    It has long been recognized that the renal proximal tubular epithelium of the hamster is a bona fide estrogen target tissue. The effect of estrogens on the growth of proximal tubule cell explants and dissociated single cells derived from these explant outgrowths has been studied in culture. Renal tubular cells were grown on a PF-HR-9 basement membrane under serum-free chemically defined culture conditions. At 7-14 days in culture, cell number was enhanced 3-fold in the presence of either 17β-estradiol or diethylstilbestrol. A similar 3-fold increase in cell number was also seen at 1 nM 17β-estradiol in subcultured dissociated single tubular cells derived from hamster renal tubular explant outgrowths at 21 days in culture. Concomitant exposure of tamoxifen at 3-fold molar excess in culture completely abolished the increase in cell number seen with 17β-estradiol. The proliferation effect of estrogens on proximal tubular cell growth appears to be species specific since 17β-estradiol did not alter the growth of either rat or guinea pig proximal tubules in culture. In addition, at 7-10 days in culture in the presence of 17β-estradiol, [ 3 H]thymidine labeling of hamster tubular cells was enhanced 3-fold. These results clearly indicate that estrogens can directly induce primary epithelial cell proliferation at physiologic concentrations and provide strong additional evidence for an important hormonal role in the neoplastic transformation of the hamster kidney

  7. Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions.

    Science.gov (United States)

    Patrikoski, Mimmi; Lee, Michelle Hui Ching; Mäkinen, Laura; Ang, Xiu Min; Mannerström, Bettina; Raghunath, Michael; Miettinen, Susanna

    2017-01-01

    Microenvironment plays an important role for stem cell proliferation and differentiation. Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro. The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and multilineage differentiation was studied under different conditions: fetal bovine serum- (FBS-) and human serum- (HS-) based media and xeno- and serum-free (XF/SF) media. Furthermore, the immunophenotype of ASCs under MMC was evaluated. The proliferative capacity of ASCs under MMC was attenuated in each condition. However, osteogenic differentiation was enhanced under MMC, shown by increased deposition of mineralized matrix in FBS and HS cultures. Likewise, significantly greater lipid droplet accumulation and increased collagen IV deposition indicated enhanced adipogenesis under MMC in FBS and HS cultures. In contrast, chondrogenic differentiation was attenuated in ASCs expanded under MMC. The ASC immunophenotype was maintained under MMC with significantly higher expression of CD54. However, MMC impaired metabolic activity and differentiation capacity of ASCs in XF/SF conditions. Both the supportive and inhibitory effects of MMC on ASC are culture condition dependent. In the presence of serum, MMC maintains ASC immunophenotype and enhances adipogenic and osteogenic differentiation at the cost of reduced proliferation.

  8. Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.

    Directory of Open Access Journals (Sweden)

    Ana Clara Mignaqui

    Full Text Available Foot-and-mouth disease (FMD is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV. The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.

  9. Purification and characterization of enterovirus 71 viral particles produced from vero cells grown in a serum-free microcarrier bioreactor system.

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    Chia-Chyi Liu

    Full Text Available BACKGROUND: Enterovirus 71 (EV71 infections manifest most commonly as a childhood exanthema known as hand-foot-and-mouth disease (HFMD and can cause neurological disease during acute infection. PRINCIPAL FINDING: In this study, we describe the production, purification and characterization of EV71 virus produced from Vero cells grown in a five-liter serum-free bioreactor system containing 5 g/L Cytodex 1 microcarrier. The viral titer was >10(6 TCID(50/mL by 6 days post infection when a MOI of 10(-5 was used at the initial infection. Two EV71 virus fractions were separated and detected when the harvested EV71 virus concentrate was purified by sucrose gradient zonal ultracentrifugation. The EV71 viral particles detected in the 24-28% sucrose fractions had an icosahedral structure 30-31 nm in diameter and had low viral infectivity and RNA content. Three major viral proteins (VP0, VP1 and VP3 were observed by SDS-PAGE. The EV71 viral particles detected in the fractions containing 35-38% sucrose were 33-35 nm in size, had high viral infectivity and RNA content, and were composed of four viral proteins (VP1, VP2, VP3 and VP4, as shown by SDS-PAGE analyses. The two virus fractions were formalin-inactivated and induced high virus neutralizing antibody responses in mouse immunogenicity studies. Both mouse antisera recognized the immunodominant linear neutralization epitope of VP1 (residues 211-225. CONCLUSION: These results provide important information for cell-based EV71 vaccine development, particularly for the preparation of working standards for viral antigen quantification.

  10. Impact of initial FDG-PET/CT and serum-free light chain on transformation of conventionally defined solitary plasmacytoma to multiple myeloma.

    Science.gov (United States)

    Fouquet, Guillemette; Guidez, Stéphanie; Herbaux, Charles; Van de Wyngaert, Zoé; Bonnet, Sarah; Beauvais, David; Demarquette, Hélène; Adib, Salim; Hivert, Bénédicte; Wemeau, Mathieu; Berthon, Céline; Terriou, Louis; Coiteux, Valérie; Macro, Margaret; Decaux, Olivier; Facon, Thierry; Huglo, Damien; Leleu, Xavier

    2014-06-15

    Solitary plasmacytoma (SP) is a localized proliferation of monoclonal plasma cells in either bone or soft tissue, without evidence of multiple myeloma (MM), and whose prognosis is marked by a high risk of transformation to MM. We studied the impact of FDG-PET/CT (2[18F]fluoro-2-deoxy-D-glucose positron emission tomography-computed tomography) on the risk of transformation of SP to overt MM among other markers in a series of 43 patients diagnosed with SP. Median age was 57.5 years; 48% of patients had an abnormal involved serum-free light chain (sFLC) value, and 64% had an abnormal sFLC ratio at diagnosis. Thirty-three percent had two or more hypermetabolic lesions on initial PET/CT, and 20% had two or more focal lesions on initial MRI. With a median follow-up of 50 months, 14 patients transformed to MM with a median time (TTMM) of 71 months. The risk factors that significantly shortened TTMM at diagnosis were two or more hypermetabolic lesions on PET/CT, abnormal sFLC ratio and involved sFLC, and to a lesser extent at completion of treatment, absence of normalized involved sFLC and PET/CT or MRI. In a multivariate analysis, abnormal initial involved sFLC [OR = 10; 95% confidence interval (CI), 1-87; P = 0.008] and PET/CT (OR = 5; 95% CI, 0-9; P = 0.032) independently shortened TTMM. An abnormal involved sFLC value and the presence of at least two hypermetabolic lesions on PET/CT at diagnosis of SP were the two predictors of early evolution to myeloma in our series. This data analysis will need confirmation in a larger study, and the study of these two risk factors may lead to a different management of patients with SP in the future. . ©2014 American Association for Cancer Research.

  11. Serum Free Light Chains in Neoplastic Monoclonal Gammopathies: Relative Under-Detection of Lambda Dominant Kappa/Lambda Ratio, and Underproduction of Free Lambda Light Chains, as Compared to Kappa Light Chains, in Patients With Neoplastic Monoclonal Gammopathies.

    Science.gov (United States)

    Lee, Won Sok; Singh, Gurmukh

    2018-07-01

    Quantitative evaluation of serum free light chains is recommended for the work up of monoclonal gammopathies. Immunoglobulin light chains are generally produced in excess of heavy chains. In patients with monoclonal gammopathy, κ/λ ratio is abnormal less frequently with lambda chain lesions. This study was undertaken to ascertain if the levels of overproduction of the two light chain types and their detection rates are different in patients with neoplastic monoclonal gammopathies. Results of serum protein electrophoresis (SPEP), serum protein immunofixation electrophoresis (SIFE), urine protein electrophoresis (UPEP), urine protein immunofixation electrophoresis (UIFE), and serum free light chain assay (SFLCA) in patients with monoclonal gammopathies were examined retrospectively. The κ/λ ratios were appropriately abnormal more often in kappa chain lesions. Ratios of κ/λ were normal in about 25% of patients with lambda chain lesions in whom free homogenous lambda light chains were detectable in urine. An illustrative case suggests underproduction of free lambda light chains, in some instances. The lower prevalence of lambda dominant κ/λ ratio in lesions with lambda light chains is estimated to be due to relative under-detection of lambda dominant κ/λ ratio in about 25% of the patients and because lambda chains are not produced in as much excess of heavy chains as are kappa chains, in about 5% of the patients. The results question the medical necessity and clinical usefulness of the serum free light chain assay. UPEP/UIFE is under-utilized.

  12. Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon

    Directory of Open Access Journals (Sweden)

    Dewi Pranatasari

    2016-06-01

    Full Text Available The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality.

  13. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.

    Directory of Open Access Journals (Sweden)

    Annie O Smith

    Full Text Available We describe a novel 3D fibrin matrix model using recombinant hematopoietic stem cell cytokines under serum-free defined conditions which promotes the assembly of human endothelial cell (EC tubes with co-associated pericytes. Individual ECs and pericytes are randomly mixed together and EC tubes form that is accompanied by pericyte recruitment to the EC tube abluminal surface over a 3-5 day period. These morphogenic processes are stimulated by a combination of the hematopoietic stem cell cytokines, stem cell factor, interleukin-3, stromal derived factor-1α, and Flt-3 ligand which are added in conjunction with fibroblast growth factor (FGF-2 into the fibrin matrix. In contrast, this tube morphogenic response does not occur under serum-free defined conditions when VEGF and FGF-2 are added together in the fibrin matrices. We recently demonstrated that VEGF and FGF-2 are able to prime EC tube morphogenic responses (i.e. added overnight prior to the morphogenic assay to hematopoietic stem cell cytokines in collagen matrices and, interestingly, they also prime EC tube morphogenesis in 3D fibrin matrices. EC-pericyte interactions in 3D fibrin matrices leads to marked vascular basement membrane assembly as demonstrated using immunofluorescence and transmission electron microscopy. Furthermore, we show that hematopoietic stem cell cytokines and pericytes stimulate EC sprouting in fibrin matrices in a manner dependent on the α5β1 integrin. This novel co-culture system, under serum-free defined conditions, allows for a molecular analysis of EC tube assembly, pericyte recruitment and maturation events in a critical ECM environment (i.e. fibrin matrices that regulates angiogenic events in postnatal life.

  14. [Cultivation of a permanent fish cell line in serum-free media special experiences with a cytotoxicity test for waste water samples

    Science.gov (United States)

    Kohlpoth, Martin; Rusche, Brigitte

    1997-01-01

    The use of fetal calf serum (FCS) as standard medium additive for the cell cultivation must be regarded critically from the point of view of animal welfare as well as for scientific reasons and makes it necessary to look for alternatives. In the last years an in vitro cytotoxicity assay for the testing of industrial waste waters with the permanent fish cell line RTG-2 was established and pre-validated as an alternative to the fish test with the golden orfe. The application of FCS is also a special problem with regard to the testing of waste waters in a cytotoxicity test so that FCS-alternatives were tested. The RTG-2 cells were successfully adapted to the two solvents Basal Medium Supplement (BMS) and Ultroser-G (U-G) that are used to replace serum. The characterisation of these adapted cell lines showed no significant differences in growth rate, adhesion rate, viability and sensitivity to chemicals in comparison to the original RTG-2 cells. On the determination of the cytotoxicity of industrial waste waters the RTG-2 cells adapted to the BMS medium indicated a clearly higher toxicity of the waste water samples than the original RTG-2 cells. This result confirms the thesis that serum components react with waste water elements and thus change the bio-availability of toxic compounds.

  15. Short-term, serum-free, static culture of cord blood-derived CD34+ cells: effects of FLT3-L and MIP-1alpha on in vitro expansion of hematopoietic progenitor cells.

    Science.gov (United States)

    Capmany, G; Querol, S; Cancelas, J A; García, J

    1999-08-01

    The use of ex vivo expanded cells has been suggested as a possible means to accelerate the speed of engraftment in cord blood (CB) transplantation. The aim of this study was to fix the optimal condition for the generation of committed progenitors without affecting the stem cell compartment. Analysis of the effects of FLT3-L and MIP-1alpha when combined with SCF, IL-3 and IL-6, in short-term (6 days), serum-free expansion cultures of CB-selected CD34+ cells. An important expansion was obtained that ranged between 8-15 times for CFU-GM, 21-51 times for the BFU-E/CFU-Mix population and 11 to 30 times for CD34+ cells assessed by flow cytometry. From the combinations tested, those in which FLT3-L was present had a significant increase in the expansion of committed progenitors, while the presence of MIP-1alpha had a detrimental effect on the generation of more differentiated cells. However, stem cell candidates assessed by week 5 CAFC assay could be maintained in culture when both MIP-1a and FLT3-L were present (up to 91% recovery). This culture system was also able to expand megakaryocytic precursors as determined by the co-expression of CD34 and CD61 antigens (45-70 times), in spite of the use of cytokines non-specific for the megakaryocytic lineage. The results obtained point to the combination of SCF, IL-3, IL-6, FLT3-L and MIP-1alpha as the best suited for a pre-clinical short-term serum-free static ex vivo expansion protocol of CB CD34+ cells, since it can generate large numbers of committed progenitor cells as well as maintaining week 5 CAFC.

  16. Smart management of sample dilution using an artificial neural network to achieve streamlined processes and saving resources: the automated nephelometric testing of serum free light chain as case study.

    Science.gov (United States)

    Ialongo, Cristiano; Pieri, Massimo; Bernardini, Sergio

    2017-02-01

    Saving resources is a paramount issue for the modern laboratory, and new trainable as well as smart technologies can be used to allow the automated instrumentation to manage samples more efficiently in order to achieve streamlined processes. In this regard the serum free light chain (sFLC) testing represents an interesting challenge, as it usually causes using a number of assays before achieving an acceptable result within the analytical range. An artificial neural network based on the multi-layer perceptron (MLP-ANN) was used to infer the starting dilution status of sFLC samples based on the information available through the laboratory information system (LIS). After the learning phase, the MLP-ANN simulation was applied to the nephelometric testing routinely performed in our laboratory on a BN ProSpec® System analyzer (Siemens Helathcare) using the N Latex FLC kit. The MLP-ANN reduced the serum kappa free light chain (κ-FLC) and serum lambda free light chain (λ-FLC) wasted tests by 69.4% and 70.8% with respect to the naïve stepwise dilution scheme used by the automated analyzer, and by 64.9% and 66.9% compared to a "rational" dilution scheme based on a 4-step dilution. Although it was restricted to follow-up samples, the MLP-ANN showed good predictive performance, which alongside the possibility to implement it in any automated system, made it a suitable solution for achieving streamlined laboratory processes and saving resources.

  17. The reduced serum free triiodothyronine and increased dorsal hippocampal SNAP-25 and Munc18-1 had existed in middle-aged CD-1 mice with mild spatial cognitive impairment.

    Science.gov (United States)

    Cao, Lei; Jiang, Wei; Wang, Fang; Yang, Qi-Gang; Wang, Chao; Chen, Yong-Ping; Chen, Gui-Hai

    2013-12-02

    Changes of synaptic proteins in highlighted brain regions and decreased serum thyroid hormones (THs) have been implied in age-related learning and memory decline. Previously, we showed significant pairwise correlations among markedly impaired spatial learning and memory ability, decreased serum free triiodothyronine (FT3) and increased hippocampal SNAP-25 and Munc18-1 in old Kunming mice. However, whether these changes and the correlations occur in middle-age mice remains unclear. Since this age is one of the best stages to study age-related cognitive decline, we explored the spatial learning and memory ability, serum THs, cerebral SNAP-25 and Munc18-1 levels and their relationships of middle-aged mice in this study. The learning and memory abilities of 35 CD-1 mice (19 mice aged 6 months and 16 mice aged 12 months) were measured with a radial six-arm water maze (RAWM). The SNAP-25 and Munc18-1 levels were semi-quantified by Western blotting and the serum THs were detected by radioimmunoassay. The results showed the middle-aged mice had decreased serum FT3, increased dorsal hippocampal (DH) SNAP-25 and Munc18-1, and many or long number of errors and latency in both learning and memory phases of the RAWM. The Pearson's correlation test showed that the DH SANP-25 and Munc18-1 levels were positively correlated with the number of errors and latency in learning phases of the RAWM. Meanwhile, the DH SANP-25 and Munc18-1 levels negatively correlated with the serum FT3 level. These results suggested that reduced FT3 with increased DH SNAP-25 and Munc18-1 levels might be involved in the spatial learning ability decline in the middle-aged mice. © 2013 Elsevier B.V. All rights reserved.

  18. RESULTS OF IN VITRO MATURATION OF MEIOTICALLY IMMATURE HUMAN OOCYTES IN A SIMPLE MEDIUM

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    Borut Kovačič

    2002-12-01

    Full Text Available Background. Among oocytes obtained during aspiration of preovulatory ovarian follicles in hormonally stimulated cycles, we ascertained the percentage of immature oocytes with the nucleus in the metaphase (M I oocytes or even in the prophase (GV oocytes of the first meiotic division and their capacity to mature in vitro in a simple medium without hormonal supplements.Methods. In 818 women, stimulated by gonadotropin releasing hormone agonist (GnRHa and gonadotropins, aspiration of preovulatory size follicles yielded 4972 oocytes. From these we denuded cells of cumulus oophorus and corona, meiotic maturity was evaluated under a microscope. Cells in the metaphase of the second meiotic division (M II oocytes and those maturing after 5 hours were used clinically in the intracytoplasmic sperm injection (ICSI procedure. Immature cells were left in the simple medium. The degree of their nuclear maturity was evaluated after one and after two days of culture. In vitro maturation was clinically used also in 14 cycles with no mature oocytes.Results. Among 4731 oocytes with denuded corona and cumulus, 4199 (88.8% were mature M II oocytes, 295 (6.2% immature M I oocytes and 237 (5% immature GV oocytes. Under in vitro conditions, 68.7% (90/131 GV oocytes attained maturity. Among M I oocytes, 63.6% (136/214 cells matured already after 5 hours and 26.6% (57/214 until the next day. In all 14 women with only immature oocytes, the embryos for embryotransfer were obtained after in vitro maturation and ICSI procedure. The result was four pregnancies and two deliveries.Conclusions. Immature oocytes, obtained in hormonally stimulated cycles, may become clinically applicable if left to mature in vitro in a simple medium without supplementation of growth factors and hormones.

  19. Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro.

    Science.gov (United States)

    Bardy, Cedric; van den Hurk, Mark; Eames, Tameji; Marchand, Cynthia; Hernandez, Ruben V; Kellogg, Mariko; Gorris, Mark; Galet, Ben; Palomares, Vanessa; Brown, Joshua; Bang, Anne G; Mertens, Jerome; Böhnke, Lena; Boyer, Leah; Simon, Suzanne; Gage, Fred H

    2015-05-19

    Human cell reprogramming technologies offer access to live human neurons from patients and provide a new alternative for modeling neurological disorders in vitro. Neural electrical activity is the essence of nervous system function in vivo. Therefore, we examined neuronal activity in media widely used to culture neurons. We found that classic basal media, as well as serum, impair action potential generation and synaptic communication. To overcome this problem, we designed a new neuronal medium (BrainPhys basal + serum-free supplements) in which we adjusted the concentrations of inorganic salts, neuroactive amino acids, and energetic substrates. We then tested that this medium adequately supports neuronal activity and survival of human neurons in culture. Long-term exposure to this physiological medium also improved the proportion of neurons that were synaptically active. The medium was designed to culture human neurons but also proved adequate for rodent neurons. The improvement in BrainPhys basal medium to support neurophysiological activity is an important step toward reducing the gap between brain physiological conditions in vivo and neuronal models in vitro.

  20. Ammonia-treated N-(1-naphthyl) ethylenediamine dihydrochloride as a novel matrix for rapid quantitative and qualitative determination of serum free fatty acids by matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yaping [Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dongdan San Tiao, Beijing 100005 (China); Wang, Yanmin [Department of Clinical Laboratory, Heze Municipal Hospital, Shandong (China); Guo, Shuai; Guo, Yumei; Liu, Hui [Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dongdan San Tiao, Beijing 100005 (China); Li, Zhili, E-mail: lizhili@ibms.pumc.edu.cn [Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dongdan San Tiao, Beijing 100005 (China)

    2013-09-10

    Graphical abstract: -- Highlights: •A novel MALDI matrix for the detection of serum free fatty acids is ammonia-treated N-(1-naphthyl) ethylenediamine dihydrochloride. •Multiple point internal standard calibration curves were constructed for nine FFAs, respectively, with excellent correlation coefficients between 0.991 and 0.999. •The MALDI-MS approach was used to rapidly differentiate the patients with and without hyperglycemia and healthy controls. -- Abstract: The blood free fatty acids (FFAs), which provide energy to the cell and act as substrates in the synthesis of fats, lipoproteins, liposaccharides, and eicosanoids, involve in a number of important physiological processes. In the present study, matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS) with ammonia-treated N-(1-naphthyl) ethylenediamine dihydrochloride (ATNEDC) as a novel MALDI matrix in a negative ion mode was employed to directly quantify serum FFAs. Multiple point internal standard calibration curves between the concentration ratios of individual fatty acids to internal standard (IS, C{sub 17:0}) versus their corresponding intensity ratios were constructed for C{sub 14:0}, C{sub 16:1}, C{sub 16:0}, C{sub 18:0}, C{sub 18:1}, C{sub 18:2}, C{sub 18:3}, C{sub 20:4}, and C{sub 22:6}, respectively, in their mixture, with correlation coefficients between 0.991 and 0.999 and limits of detection (LODs) between 0.2 and 5.4 μM, along with the linear dynamic range of more than two orders of magnitude. The results indicate that the multiple point internal standard calibration could reduce the impact of ion suppression and improve quantification accuracy in the MALDI mode. The quantitative results of nine FFAs from 339 serum samples, including 161 healthy controls, 118 patients with hyperglycemia and 60 patients without hyperglycemia show that FFAs levels in hyperglycemic patient sera are significantly higher than those in healthy

  1. Ammonia-treated N-(1-naphthyl) ethylenediamine dihydrochloride as a novel matrix for rapid quantitative and qualitative determination of serum free fatty acids by matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry

    International Nuclear Information System (INIS)

    Zhang, Yaping; Wang, Yanmin; Guo, Shuai; Guo, Yumei; Liu, Hui; Li, Zhili

    2013-01-01

    Graphical abstract: -- Highlights: •A novel MALDI matrix for the detection of serum free fatty acids is ammonia-treated N-(1-naphthyl) ethylenediamine dihydrochloride. •Multiple point internal standard calibration curves were constructed for nine FFAs, respectively, with excellent correlation coefficients between 0.991 and 0.999. •The MALDI-MS approach was used to rapidly differentiate the patients with and without hyperglycemia and healthy controls. -- Abstract: The blood free fatty acids (FFAs), which provide energy to the cell and act as substrates in the synthesis of fats, lipoproteins, liposaccharides, and eicosanoids, involve in a number of important physiological processes. In the present study, matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS) with ammonia-treated N-(1-naphthyl) ethylenediamine dihydrochloride (ATNEDC) as a novel MALDI matrix in a negative ion mode was employed to directly quantify serum FFAs. Multiple point internal standard calibration curves between the concentration ratios of individual fatty acids to internal standard (IS, C 17:0 ) versus their corresponding intensity ratios were constructed for C 14:0 , C 16:1 , C 16:0 , C 18:0 , C 18:1 , C 18:2 , C 18:3 , C 20:4 , and C 22:6 , respectively, in their mixture, with correlation coefficients between 0.991 and 0.999 and limits of detection (LODs) between 0.2 and 5.4 μM, along with the linear dynamic range of more than two orders of magnitude. The results indicate that the multiple point internal standard calibration could reduce the impact of ion suppression and improve quantification accuracy in the MALDI mode. The quantitative results of nine FFAs from 339 serum samples, including 161 healthy controls, 118 patients with hyperglycemia and 60 patients without hyperglycemia show that FFAs levels in hyperglycemic patient sera are significantly higher than those in healthy controls and patients without

  2. Role of hemolysis in potassium release by iodinated contrast medium

    Energy Technology Data Exchange (ETDEWEB)

    Hayakawa, K.; Nakamura, T.; Shimizu, Y. [Department of Radiology, Kyoto City Hospital (Japan)

    1999-09-01

    It has been demonstrated that an iodinated contrast medium (CM) causes release of potassium into blood vessel lumina, resulting in an increase in serum potassium. The purpose of the present study was to assess whether this potassium release is due to hemolysis. Fresh human blood was mixed in vitro with CM at a ratio of 10:2. Potassium release rates were determined, and serum haptoglobin and free hemoglobin were measured after 30 min of exposure to CM. To compare the potassium release curve between CM exposure and true hemolysis induced by distilled water, fresh human blood was also mixed with distilled water. The level of serum haptoglobin decreased due to hemodilution. Changes in haptoglobin were not correlated with potassium release rates. The serum free hemoglobin level did not increase significantly, and there was no correlation between changes in the free hemoglobin level and the rate of potassium release. Hemolysis caused by water occurred instantaneously, whereas potassium release caused by CM was a slow response, which was linearly correlated with exposure time. Potassium release from blood cannot be explained by hemolysis. (orig.) With 4 figs., 4 tabs., 3 refs.

  3. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays

    International Nuclear Information System (INIS)

    Walter, M.N.M.; Wright, K.T.; Fuller, H.R.; MacNeil, S.; Johnson, W.E.B.

    2010-01-01

    We have used in vitro scratch assays to examine the relative contribution of dermal fibroblasts and keratinocytes in the wound repair process and to test the influence of mesenchymal stem cell (MSC) secreted factors on both skin cell types. Scratch assays were established using single cell and co-cultures of L929 fibroblasts and HaCaT keratinocytes, with wound closure monitored via time-lapse microscopy. Both in serum supplemented and serum free conditions, wound closure was faster in L929 fibroblast than HaCaT keratinocyte scratch assays, and in co-culture the L929 fibroblasts lead the way in closing the scratches. MSC-CM generated under serum free conditions significantly enhanced the wound closure rate of both skin cell types separately and in co-culture, whereas conditioned medium from L929 or HaCaT cultures had no significant effect. This enhancement of wound closure in the presence of MSC-CM was due to accelerated cell migration rather than increased cell proliferation. A number of wound healing mediators were identified in MSC-CM, including TGF-β1, the chemokines IL-6, IL-8, MCP-1 and RANTES, and collagen type I, fibronectin, SPARC and IGFBP-7. This study suggests that the trophic activity of MSC may play a role in skin wound closure by affecting both dermal fibroblast and keratinocyte migration, along with a contribution to the formation of extracellular matrix.

  4. A novel liquid medium for the efficient growth of the salmonid pathogen Piscirickettsia salmonis and optimization of culture conditions.

    Directory of Open Access Journals (Sweden)

    Mirtha Henríquez

    Full Text Available Piscirickettsia salmonis is the bacterium that causes Piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. The growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eukaryotic cell lines, a process that involves high production costs and is time-consuming. Recent research has demonstrated that it is possible to culture pure P. salmonis in a blood containing (cell-free medium. In the present work we demonstrate the growth of P. salmonis in a liquid medium free from blood and serum components, thus establishing a novel and simplified bacteriological medium. Additionally, the new media reported provides improved growth conditions for P. salmonis, where biomass concentrations of approximately 800 mg cell dry weight L(-1 were obtained, about eight times higher than those reported for the blood containing medium. A 2- level full factorial design was employed to evaluate the significance of the main medium components on cell growth and an optimal temperature range of 23-27°C was determined for the microorganism to grow in the novel liquid media. Therefore, these results represent a breakthrough regarding P. salmonis research in order to optimize pure P. salmonis growth in liquid blood and serum free medium.

  5. Concept medium programme

    DEFF Research Database (Denmark)

    Bjerrum, Peter

    2005-01-01

    The present essay is an attempt to determine the architectural project of the 21st century in relation to a modern conception of space as the medium of architecture, and of sociality as its program......The present essay is an attempt to determine the architectural project of the 21st century in relation to a modern conception of space as the medium of architecture, and of sociality as its program...

  6. The Interstellar Medium

    CERN Document Server

    Lequeux, James

    2005-01-01

    Describing interstellar matter in our galaxy in all of its various forms, this book also considers the physical and chemical processes that are occurring within this matter. The first seven chapters present the various components making up the interstellar matter and detail the ways that we are able to study them. The following seven chapters are devoted to the physical, chemical and dynamical processes that control the behaviour of interstellar matter. These include the instabilities and cloud collapse processes that lead to the formation of stars. The last chapter summarizes the transformations that can occur between the different phases of the interstellar medium. Emphasizing methods over results, "The Interstellar Medium" is written for graduate students, for young astronomers, and also for any researchers who have developed an interest in the interstellar medium.

  7. Optical recording medium

    International Nuclear Information System (INIS)

    Andriech, A.; Bivol, V.; Tridukh, G.; Tsiuleanu, D.

    2002-01-01

    The invention relates of the micro- and optoelectronics, computer engineering ,in particular, to tjhe optical information media and may be used in hilography. Summary of the invention consists in that the optical image recording medium, containing a dielectric substrates, onto one surface of which there are placed in series a transparent electricity conducting layer, a photo sensitive recording layer of chalcogenic glass and a thin film electrode of aluminium, is provided with an optically transparent protective layer, applied into the thin film electrode. The result of the invention consists in excluding the dependence of chemical processes course into the medium upon environmental conditions

  8. Maturation of pig oocytes in vitro in a medium with pyruvate

    Directory of Open Access Journals (Sweden)

    H. Gonzales-Figueroa

    2005-06-01

    Full Text Available The aim of in vitro maturation oocyte systems is to produce oocytes of comparable quality to those derived in vivo. The present study was designed to examine the surface morphological changes of the cumulus-oocyte complex (COC and nuclear maturation in a culture system containing pyruvate. Ovaries were obtained from a slaughterhouseand transported to the laboratory within 2 h at 35-39ºC,and rinsed three times in 0.9% NaCl. The COCs were harvested from the ovaries and in vitro maturation was evaluated in San Marcos (SM medium, a chemically defined culture system containing 22.3 mM sodium pyruvate. Oocytes were cultured in SM, SM + porcine follicular fluid (pFF and in SM + pFF + gonadotropins (eCG and hCG for 20-22 h and then without hormonal supplements for an additional 20-22 h. After culture, the degree of cumulus expansion and frequency of nuclear maturation were determined. Oocytes matured in SM (40.9% and SM + pFF (42.9% showed moderate cumulus expansion, whereas oocytes matured in SM + pFF + gonadotropins (54.6% showed high cumulus expansion. The maturation rate of cultured oocytes, measured in function of the presence of the polar corpuscle, did not differ significantly between SM (40.9 ± 3.6% and SM + pFF (42.9 ± 3.7%. These results indicate that pig oocytes can be successfully matured in a chemically definedmedium and suggest a possible bifunctional role of pyruvate as an energy substrate and as an antioxidant protecting oocytes against the stress of the in vitro environment.

  9. Roentgen contrast medium

    International Nuclear Information System (INIS)

    Tamborski, C.

    1989-01-01

    The patent deals with a roentgen contrast medium containing a perfluorinebrominealkylether of the formula C m F 2m+1 OC n F 2n Br dispersed in water, preferentially in the presence of a non-ionic dispersing agent such as a fluorinated amidoaminoxide. 2 tabs

  10. Radon in geological medium

    Energy Technology Data Exchange (ETDEWEB)

    Hricko, J [GEOCOMPLEX, a.s., Bratislava (Slovakia)

    1996-12-31

    The paper presented deals with behavior of the radon in geological medium and with some results of the radon survey in Bratislava and Kosice regions. 1) The a{sub v} has been detected in the holes 0.80 m deep. The density of observations - 3 reference areas (one represents 20 stations) per 1 km{sup 2}. The radon risk maps in 1:25000 and 1:50000 scales have been compiled. The 56.8% of the project area lies in low radon risk, 37.6% in medium radon risk and 5.6% in high radon risk. Follow-up monitoring of the equivalent volume radon activity (EVRA) at the flats, located in the areas with high radon risk of the surface layer, has showed values several times higher than Slovak limits (Marianka, Raca, Vajnory). The evidence that neotectonic is excellent medium for rising up emanation to the subsurface layer, is shown on the map. The tectonic zone of Liscie udolie in Bratislava-Karlova Ves area has been clearly detected by profile radon survey (a{sub v} > 50 kBq/m{sup 3}). 2) At present, northern half of the area of Kosice in question was covered by radon survey. The low and medium radon risks have been observed here, while localities with high radon risk are small in extent. The part of radon risk and soil permeability map from northern Kosice area is shown. (J.K.) 3 figs., 2 refs.

  11. Radon in geological medium

    International Nuclear Information System (INIS)

    Hricko, J.

    1995-01-01

    The paper presented deals with behavior of the radon in geological medium and with some results of the radon survey in Bratislava and Kosice regions. 1) The a v has been detected in the holes 0.80 m deep. The density of observations - 3 reference areas (one represents 20 stations) per 1 km 2 . The radon risk maps in 1:25000 and 1:50000 scales have been compiled. The 56.8% of the project area lies in low radon risk, 37.6% in medium radon risk and 5.6% in high radon risk. Follow-up monitoring of the equivalent volume radon activity (EVRA) at the flats, located in the areas with high radon risk of the surface layer, has showed values several times higher than Slovak limits (Marianka, Raca, Vajnory). The evidence that neotectonic is excellent medium for rising up emanation to the subsurface layer, is shown on the map. The tectonic zone of Liscie udolie in Bratislava-Karlova Ves area has been clearly detected by profile radon survey (a v > 50 kBq/m 3 ). 2) At present, northern half of the area of Kosice in question was covered by radon survey. The low and medium radon risks have been observed here, while localities with high radon risk are small in extent. The part of radon risk and soil permeability map from northern Kosice area is shown. (J.K.) 3 figs., 2 refs

  12. Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.

    Science.gov (United States)

    Sidney, Laura E; Branch, Matthew J; Dua, Harminder S; Hopkinson, Andrew

    2015-12-01

    The limbal area of the corneal stroma has been identified as a source of mesenchymal-like stem cells, which have potential for exploitation as a cell therapy. However, the optimal culture conditions are disputed and few direct media comparisons have been performed. In this report, we evaluated several media types to identify the optimal for inducing an in vitro stem cell phenotype. Primary human corneal stroma-derived stem cells (CSSCs) were extracted from corneoscleral rims. Culture in seven different media types was compared: Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS); M199 with 20% FBS; DMEM-F12 with 20% serum replacement, basic fibroblast growth factor and leukemia inhibitory factor (SCM); endothelial growth medium (EGM); semi-solid MethoCult; serum-free keratinocyte medium (K-SFM); and StemPro-34. Effects on proliferation, morphology, protein and messenger RNA expression were evaluated. All media supported proliferation of CSSCs with the exception of K-SFM and StemPro-34. Morphology differed between media: DMEM produced large cells, whereas EGM produced very small cells. Culture in M199 produced a typical mesenchymal stromal cell phenotype with high expression of CD105, CD90 and CD73 but not CD34. Culture in SCM produced a phenotype more reminiscent of a progenitor cell type with expression of CD34, ABCG2, SSEA-4 and PAX6. Culture medium can significantly influence CSSC phenotype. SCM produced a cell phenotype closest to that of a pluripotent stem cell, and we consider it to be the most appropriate for development as a clinical-grade medium for the production of CSSC phenotypes suitable for cell therapy. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  13. Concept medium program

    DEFF Research Database (Denmark)

    Bjerrum, Peter

    2003-01-01

    The present essays is an attempt to dertermine the architecural project of the 21st century in realation to a modern conception of space as the medium of architecture, and of society as its program. This attempt adopts the internal point of view of an architect in describing a modern architectural...... project within the framework: concept - program, these notions being concieved as spatial representations primarily and immediately "given" to architecture....

  14. Influence of serum extraction from the culture medium and of sublethal X-ray irradiation upon microvilli and invaginations of the membrane of Ehrlich ascites tumor cells in monolayer culture

    International Nuclear Information System (INIS)

    Laudenbach, G.; Pfab, R.; Hess, F.; Schachtschabel, D.O.

    1984-01-01

    In order to find out modifications of microvilli and invaginations, the cellular surfaces of Ehrlich ascites tumor cells in monolayer culture (basal medium of Eagle + 10% fetal calf serum) were investigated with the aid of electron-microscopic cross-sections. The tumor cells had been cultured without serum 24 hours prior to investigation or irradiated with 2 Gy. Morphometric evaluation after cell culture in a serum-free medium showed a reduced number of microvilli and a diminution of sections of microvilli. As already described before, a reduction of cell proliferation, of the microtubule-microfilament system, and of the endocytosis activity occurs under these serum-free conditions. The number of invaginations (related to a constant membrane part) was reduced by nearly 50% after serum extraction. Similarly to serum extraction, sublethal X-ray irradiation reduced the sections of microvilli, whereas the number of microvilli increased slightly. Contrary to the effect of serum extraction, the irradiated cells showed twice as many invaginations as the non-irradiated control cells. These differences in the surface structures are interpreted as a result of modified growth stimulations (+- serum) and radiogenic reparation processes. (orig.) [de

  15. DENSE MEDIUM CYCLONE OPTIMIZATON

    Energy Technology Data Exchange (ETDEWEB)

    Gerald H. Luttrell; Chris J. Barbee; Peter J. Bethell; Chris J. Wood

    2005-06-30

    Dense medium cyclones (DMCs) are known to be efficient, high-tonnage devices suitable for upgrading particles in the 50 to 0.5 mm size range. This versatile separator, which uses centrifugal forces to enhance the separation of fine particles that cannot be upgraded in static dense medium separators, can be found in most modern coal plants and in a variety of mineral plants treating iron ore, dolomite, diamonds, potash and lead-zinc ores. Due to the high tonnage, a small increase in DMC efficiency can have a large impact on plant profitability. Unfortunately, the knowledge base required to properly design and operate DMCs has been seriously eroded during the past several decades. In an attempt to correct this problem, a set of engineering tools have been developed to allow producers to improve the efficiency of their DMC circuits. These tools include (1) low-cost density tracers that can be used by plant operators to rapidly assess DMC performance, (2) mathematical process models that can be used to predict the influence of changes in operating and design variables on DMC performance, and (3) an expert advisor system that provides plant operators with a user-friendly interface for evaluating, optimizing and trouble-shooting DMC circuits. The field data required to develop these tools was collected by conducting detailed sampling and evaluation programs at several industrial plant sites. These data were used to demonstrate the technical, economic and environmental benefits that can be realized through the application of these engineering tools.

  16. Computation as Medium

    DEFF Research Database (Denmark)

    Jochum, Elizabeth Ann; Putnam, Lance

    2017-01-01

    Artists increasingly utilize computational tools to generate art works. Computational approaches to art making open up new ways of thinking about agency in interactive art because they invite participation and allow for unpredictable outcomes. Computational art is closely linked...... to the participatory turn in visual art, wherein spectators physically participate in visual art works. Unlike purely physical methods of interaction, computer assisted interactivity affords artists and spectators more nuanced control of artistic outcomes. Interactive art brings together human bodies, computer code......, and nonliving objects to create emergent art works. Computation is more than just a tool for artists, it is a medium for investigating new aesthetic possibilities for choreography and composition. We illustrate this potential through two artistic projects: an improvisational dance performance between a human...

  17. Spiegel. Medium. Kunst

    DEFF Research Database (Denmark)

    Kacunko, Slavko

    of this kind as the marks of a meta-complex of method out of which new models of the image continually arise. The waxing intangibility of proliferating images be they of the mind, in dreams, through gestures, and the equally rampant growth of microstructuring in allocations of knowledge lay a challenge before...... to research. As a void in the apprehension of the world, the mirror obtained a scholarly perspective and the more so in areas beyond its own qualities as a medium, i.e. in images and metaphor, the paradigms of all research looking to image and text. This investigation sets out to comprehend paradoxes......, and space (albeit no expanse) is there to consider the methodological pros and cons of such a selective approach. Even in this synopsis, it has to be and can be stated that what makes for the affinities between psychological, literary and image-research approaches in research on the mirror is the shared...

  18. The diffuse interstellar medium

    Science.gov (United States)

    Cox, Donald P.

    1990-01-01

    The last 20 years of the efforts to understand the diffuse ISM are reviewed, with recent changes of fundamental aspects being highlighted. Attention is given to the interstellar pressure and its components, the weight of the ISM, the midplane pressure contributions, and pressure contributions at 1 kpc. What velocity dispersions, cosmic ray pressure, and magnetic field pressure that can be expected for a gas in a high magnetic field environment is addressed. The intercloud medium is described, with reference to the work of Cox and Slavin (1989). Various caveats are discussed and a number of areas for future investigation are identified. Steps that could be taken toward a successful phase segregation model are discussed.

  19. Supplementation of serum free media with HT is not sufficient to restore growth properties of DHFR-/- cells in fed-batch processes - Implications for designing novel CHO-based expression platforms.

    Science.gov (United States)

    Florin, Lore; Lipske, Carolin; Becker, Eric; Kaufmann, Hitto

    2011-04-10

    DHFR-deficient CHO cells are the most commonly used host cells in the biopharmaceutical industry and over the years, individual substrains have evolved, some have been engineered with improved properties and platform technologies have been designed around them. Unexpectedly, we have observed that different DHFR-deficient CHO cells show only poor growth in fed-batch cultures even in HT supplemented medium, whereas antibody producer cells derived from these hosts achieved least 2-3 fold higher peak cell densities. Using a set of different expression vectors, we were able to show that this impaired growth performance was not due to the selection procedure possibly favouring fast growing clones, but a direct consequence of DHFR deficiency. Re-introduction of the DHFR gene reproducibly restored the growth phenotype to the level of wild-type CHO cells or even beyond which seemed to be dose-dependent. The requirement for a functional DHFR gene to achieve optimal growth under production conditions has direct implications for cell line generation since it suggests that changing to a selection system other than DHFR would require another CHO host which - especially for transgenic CHO strains and tailor-suited process platforms - this could mean significant investments and potential changes in product quality. In these cases, DHFR engineering of the current CHO-DG44 or DuxB11-based host could be an attractive alternative. Copyright © 2011 Elsevier B.V. All rights reserved.

  20. Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response.

    Science.gov (United States)

    Haniu, Hisao; Saito, Naoto; Matsuda, Yoshikazu; Tsukahara, Tamotsu; Maruyama, Kayo; Usui, Yuki; Aoki, Kaoru; Takanashi, Seiji; Kobayashi, Shinsuke; Nomura, Hiroki; Okamoto, Masanori; Shimizu, Masayuki; Kato, Hiroyuki

    2013-09-01

    We examined the cytotoxicity of multi-walled carbon nanotubes (MWCNTs) and the resulting cytokine secretion in BEAS-2B cells or normal human bronchial epithelial cells (HBEpCs) in two types of culture media (Ham's F12 containing 10% FBS [Ham's F12] and serum-free growth medium [SFGM]). Cellular uptake of MWCNT was observed by fluorescent microscopy and analyzed using flow cytometry. Moreover, we evaluated whether MWCNT uptake was suppressed by 2 types of endocytosis inhibitors. We found that BEAS-2B cells cultured in Ham's F12 and HBEpCs cultured in SFGM showed similar biological responses, but BEAS-2B cells cultured in SFGM did not internalize MWCNTs, and the 50% inhibitory concentration value, i.e., the cytotoxicity, was increased by more than 10-fold. MWCNT uptake was suppressed by a clathrin-mediated endocytosis inhibitor and a caveolae-mediated endocytosis inhibitor in BEAS-2B cells cultured in Ham's F12 and HBEpCs cultured in SFGM. In conclusion, we suggest that BEAS-2B cells cultured in a medium containing serum should be used for the safety evaluation of nanomaterials as a model of normal human bronchial epithelial cells. However, the culture medium composition may affect the proteins that are expressed on the cytoplasmic membrane, which may influence the biological response to MWCNTs. Copyright © 2013 The Authors. Published by Elsevier Ltd.. All rights reserved.

  1. Apoptosis in chondrogenesis of human mesenchymal stem cells: effect of serum and medium supplements.

    Science.gov (United States)

    Wang, Chien-Yuan; Chen, Ling-Lan; Kuo, Pei-Yin; Chang, Jia-Ling; Wang, Yng-Jiin; Hung, Shih-Chieh

    2010-04-01

    Apoptosis is an inevitable process during development and is evident in the formation of articular cartilage and endochondral ossification of growth plate. Mesenchymal stem cells (MSCs) can serve as alternative sources for cell therapy in focal chondral lesions or diffuse osteoarthritis. But there are few, if any, studies investigating apoptosis during chondrogenesis by MSCs. The aim of this study was to find the better condition to prevent apoptosis during chondrogenesis by MSCs. Apoptosis were evaluated in MSCs induced in different chondrogenic media by the use of Annexin V, TUNEL staining, lysosomal labeling with lysotracker and immunostaining of apoptotic markers. We found apparent apoptosis was demonstrated by Annexin V, TUNEL staining and lysosomal labeling during chondrogenesis. Meanwhile, the degree of apoptosis was related to the reagents of the defined chondrogenic medium. Adding serum in medium increased apoptosis, however, TGF-beta1 inhibited apoptosis. The apoptosis was associated with the activation of caspase-3, the increase in the Bax/Bcl-2 ratio, the loss of lysosomal integrity, and the increase of PARP-cleavage. Pro-inflammatory cytokines, IL-1alpha, IL-1beta and TNFalpha did not induce any increase in apoptosis. Interestingly, the inhibition of apoptosis by serum free medium supplemented with ITS was also associated with an increase in the expression of type II collagen, and a decrease in the expression of type X collagen, Runx2, and other osteogenic genes, while TGF-beta1 increased the expression of Sox9, type II and type X collagen and decreased the expression of osteogenic genes. These data suggest apoptosis occurs during chondrogenesis by MSCs by cell death intrinsic pathway activation and this process may be modulated by culture conditions.

  2. Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures.

    Science.gov (United States)

    Bohlen, Christopher J; Bennett, F Chris; Tucker, Andrew F; Collins, Hannah Y; Mulinyawe, Sara B; Barres, Ben A

    2017-05-17

    Microglia, the resident macrophages of the CNS, engage in various CNS-specific functions that are critical for development and health. To better study microglia and the properties that distinguish them from other tissue macrophage populations, we have optimized serum-free culture conditions to permit robust survival of highly ramified adult microglia under defined-medium conditions. We find that astrocyte-derived factors prevent microglial death ex vivo and that this activity results from three primary components, CSF-1/IL-34, TGF-β2, and cholesterol. Using microglial cultures that have never been exposed to serum, we demonstrate a dramatic and lasting change in phagocytic capacity after serum exposure. Finally, we find that mature microglia rapidly lose signature gene expression after isolation, and that this loss can be reversed by engrafting cells back into an intact CNS environment. These data indicate that the specialized gene expression profile of mature microglia requires continuous instructive signaling from the intact CNS. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. Mesons in the nuclear Medium

    CERN Document Server

    Kotulla, M

    2006-01-01

    We discuss recent experimental results on the modification of hadron properties in a nuclear medium. Particular emphasis is placed on an $\\omega$ production experiment performed by the CBELSA/TAPS collaboration at the ELSA accelerator. The data shows a smaller $\\omega$ meson mass together with a significant increase of its width in the nuclear medium.

  4. Pions in the nuclear medium

    International Nuclear Information System (INIS)

    Chanfray, G.

    1996-07-01

    We discuss various aspects of pion physics in the nuclear medium. We first study s-wave pion-nucleus interaction in connection with chiral symmetry restoration and quark condensate in the nuclear medium. We then address the question of p-wave pion-nucleus interaction and collective pionic modes in nuclei and draw the consequences for in medium ππ correlations especially in the scalar-isoscalar channel. We finally discuss the modification of the rho meson mass spectrum at finite density and/or temperature in connection with relativistic heavy ion collisions

  5. Medium modifications of vector mesons

    International Nuclear Information System (INIS)

    Pant, L.M.

    2004-01-01

    The omega photoproduction in nuclear medium with the ELSA facility at Bonn and the present status of the HADES collaboration to investigate the in-medium hadron properties in proton, heavy ions and hadron induced reactions at GSI, Darmstadt are presented. Efforts are under way to utilise the electron beam at Indore for experimental hadron physics in order to step into the intermediate energy nuclear physics regime. The skeletal outline of the high energy electron beam now available at CAT, Indore is discussed

  6. Medium effects in direct reactions

    International Nuclear Information System (INIS)

    Karakoc, M; Bertulani, C

    2013-01-01

    We discuss medium corrections of the nucleon-nucleon (NN) cross sections and their influence on direct reactions at intermediate energies ≳50 MeV/nucleon. The results obtained with free NN cross sections are compared with those obtained with a geometrical treatment of Pauli-blocking and Dirac-Bruecker methods. We show that medium corrections may lead to sizable modifications for collisions at intermediate energies and that they are more pronounced in reactions involving weakly bound nuclei.

  7. Can serum free fatty acids assessment predict severe preeclampsia ...

    African Journals Online (AJOL)

    Evaluation of serum fasting FFAs, uric acid, liver transaminases (AST, ALT) during delivery were done. Results: The mean level of FFAs was significantly elevated in preeclampsia cases compared to women with normal blood pressure (2.12 ± 2.64, 0.43± 0.29 respectively, p= 0.003). Also, cases with high FFAs levels had ...

  8. Can serum free fatty acids assessment predict severe preeclampsia?

    African Journals Online (AJOL)

    Nermeen Saad El Beltagy

    2011-10-20

    Oct 20, 2011 ... Methods: Twenty cases with severe preeclampsia (blood pressure P 160/110 after 20th week of ges- tation and ... ing factor with preeclampsia in non-obese pregnant women. ... Preeclampsia (PE) is a common pregnancy disorder that is ... centration of free fatty acids in the serum was measured by an.

  9. A simplified ultrafiltration method for determination of serum free cortisol

    International Nuclear Information System (INIS)

    MacMahon, W.; Sgoutas, D.

    1983-01-01

    The authors describe the suitability of the Amicon MPS-1 centrifugal ultrafiltration device and the YMB membrane for measuring free cortisol in serum. The method combines two independent assays: total cortisol and the ultrafiltrate fraction of added [ 3 H]cortisol. The unbound fraction is determined in 0.25-0.30 ml of ultrafiltrate collected from 0.6 to 1 ml of serum that has been equilibrated with [ 3 H]cortisol at 37 0 C for 20 min. The assay is rapid (less than 1 h), practical (no more than 0.6 ml of serum is necessary) and repeatable (CV: 3.8% within-assay and 12.2% in different assays). Error introduced in free cortisol measurement due to dilution effects in dialysis is systematically defined, and the effect of tracer purity on the ultrafiltration method is examined. Dialyzed sera from normal men and women, from patients with Cushing's disease and adrenal insufficiency, and from pregnant women gave ultrafiltration results that accurately duplicated those obtained by previous dialysis. (Auth.)

  10. Measurements of serum-free thyroid hormone concentrations by ultrafiltration

    International Nuclear Information System (INIS)

    Konno, Norimichi; Hagiwara, Kohji; Taguchi, Hideo; Murakami, Shigeki; Taguchi, Shizuko

    1987-01-01

    An ultrafiltration method (UF) for measuring free thyroxine (FT 4 ) and free triiodothyronine (FT 3 ) using the Diaflow YM membrane (Centricon-10) is described. The results are compared with those by equilibrium dialysis (ED) and also by mathematical calculations derived from T 4 , T 3 , and binding protein concentrations. The precision with the UF method was excellent. The normal ranges of FT 4 and FT 3 by the three methods are all comparable. There was a high degree of correlation of FT 4 or FT 3 results by UF with those by ED and by calculation (r = 0.940 - 0.974, n = 161, P 4 and FT 3 by all methods agreed well for hyperthyroidism, hypothyroidism, and for patients with low T 4 -binding globulin. The mean FT 3 in pregnancy was lower than the normal value for all methods, and FT 4 concentrations by UF and calculation also decreased in late pregnancy. The mean FT 4 by UF and ED in low T 3 syndrome were significantly higher than in the normal controls, while the calculated FT 4 was lower. The FT 3 in low T 3 syndrome distributed normal to subnormal in all methods. These results indicate that a) the UF method is a reliable reference method for measuring FT 4 and FT 3 concentrations; b) the UF results agree well with those by ED and also with theoretically derived values in subjects with thyroid diseases and TBG abnormalities; c) for patients with low T 3 syndrome, the FT 4 results obtained by UF and ED are similarly discrepant from the calculated results, implying the existence of binding inhibitor(s) which affect both UF and ED measurements. (author)

  11. Properties of the nuclear medium

    International Nuclear Information System (INIS)

    Baldo, M; Burgio, G F

    2012-01-01

    We review our knowledge on the properties of the nuclear medium that have been studied, over many years, on the basis of many-body theory, laboratory experiments and astrophysical observations. Throughout the presentation particular emphasis is placed on the possible relationship and links between the nuclear medium and the structure of nuclei, including the limitations of such an approach. First we consider the realm of phenomenological laboratory data and astrophysical observations and the hints they can give on the characteristics that the nuclear medium should possess. The analysis is based on phenomenological models, that however have a strong basis on physical intuition and an impressive success. More microscopic models are also considered, and it is shown that they are able to give invaluable information on the nuclear medium, in particular on its equation of state. The interplay between laboratory experiments and astrophysical observations is particularly stressed, and it is shown how their complementarity enormously enriches our insights into the structure of the nuclear medium. We then introduce the nucleon–nucleon interaction and the microscopic many-body theory of nuclear matter, with a critical discussion about the different approaches and their results. The Landau–Fermi liquid theory is introduced and briefly discussed, and it is shown how fruitful it can be in discussing the macroscopic and low-energy properties of the nuclear medium. As an illustrative example, we discuss neutron matter at very low density, and it is shown how it can be treated within the many-body theory. The general bulk properties of the nuclear medium are reviewed to indicate at which stage of our knowledge we stand, taking into account the most recent developments both in theory and experiments. A section is dedicated to the pairing problem. The connection with nuclear structure is then discussed, on the basis of the energy density functional method. The possibility of

  12. 1 Medium Regiment, (SAHA), SAA

    African Journals Online (AJOL)

    Scientia Militaria: South African Journal of Military Studies. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 16, No 4 (1986) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register. 1 Medium Regiment, (S.A.H.A.), ...

  13. Hadron photoproduction at medium energy

    International Nuclear Information System (INIS)

    Dainton, J.B.

    1985-04-01

    Results from measurements of multibody photoproduction at medium incident photon energy (2.8 to 4.8 GeV) are presented and discussed. Particular emphasis is placed on topics which are not well understood and which therefore motivate experiments with the upgraded electron accelerator and storage ring ELSA at the University of Bonn, FR Germany. (author)

  14. Animal Locomotion in Different Mediums

    Indian Academy of Sciences (India)

    Wetlands are repositories of unique biodiversity. Wetlandorganisms are well adapted to their habitat, lying at theinterface of aquatic and terrestrial environments. In order tounderstand their adaptations in a better way, it is essential tograsp the basic properties of the medium in which variousorganisms live. This is attempted ...

  15. Conditioned medium from the stem cells of human dental pulp improves cognitive function in a mouse model of Alzheimer's disease.

    Science.gov (United States)

    Mita, Tsuneyuki; Furukawa-Hibi, Yoko; Takeuchi, Hideyuki; Hattori, Hisashi; Yamada, Kiyofumi; Hibi, Hideharu; Ueda, Minoru; Yamamoto, Akihito

    2015-10-15

    Alzheimer's disease (AD) is a progressive, neurodegenerative disease characterized by a decline in cognitive abilities and the appearance of β-amyloid plaques in the brain. Although the pathogenic mechanisms associated with AD are not fully understood, activated microglia releasing various neurotoxic factors, including pro-inflammatory cytokines and oxidative stress mediators, appear to play major roles. Here, we investigated the therapeutic benefits of a serum-free conditioned medium (CM) derived from the stem cells of human exfoliated deciduous teeth (SHEDs) in a mouse model of AD. The intranasal administration of SHEDs in these mice resulted in substantially improved cognitive function. SHED-CM contained factors involved in multiple neuroregenerative mechanisms, such as neuroprotection, axonal elongation, neurotransmission, the suppression of inflammation, and microglial regulation. Notably, SHED-CM attenuated the pro-inflammatory responses induced by β-amyloid plaques, and generated an anti-inflammatory/tissue-regenerating environment, which was accompanied by the induction of anti-inflammatory M2-like microglia. Our data suggest that SHED-CM may provide significant therapeutic benefits for AD. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Holographic Renormalization in Dense Medium

    International Nuclear Information System (INIS)

    Park, Chanyong

    2014-01-01

    The holographic renormalization of a charged black brane with or without a dilaton field, whose dual field theory describes a dense medium at finite temperature, is investigated in this paper. In a dense medium, two different thermodynamic descriptions are possible due to an additional conserved charge. These two different thermodynamic ensembles are classified by the asymptotic boundary condition of the bulk gauge field. It is also shown that in the holographic renormalization regularity of all bulk fields can reproduce consistent thermodynamic quantities and that the Bekenstein-Hawking entropy is nothing but the renormalized thermal entropy of the dual field theory. Furthermore, we find that the Reissner-Nordström AdS black brane is dual to a theory with conformal matter as expected, whereas a charged black brane with a nontrivial dilaton profile is mapped to a theory with nonconformal matter although its leading asymptotic geometry still remains as AdS space

  17. Medium modifications with recoil polarization

    Energy Technology Data Exchange (ETDEWEB)

    Brand, J.F.J. van den [Nationaal Instituut voor Kernfysica en Hoge Energiefysica, Amsterdam (Netherlands); Ent, R. [CEBAF, Newport News, VA (United States)

    1994-04-01

    The authors show that the virtual Compton scattering process allows for a precise study of the off-shell electron-nucleon vertex. In a separable model, they show the sensitivity to new unconstrained structure functions of the nucleon, beyond the usual Dirac and Pauli form factors. In addition, they show the sensitivity to bound nucleon form factors using the reaction 4He({rvec e},e{prime},{rvec p}){sup 3}H. A nucleon embedded in a nucleus represents a complex system. Firstly, the bound nucleon is necessarily off-shell and in principle a complete understanding of the dynamical structure of the nucleon is required in order to calculate its off-shell electromagnetic interaction. Secondly, one faces the possibility of genuine medium effects, such as for example quark-exchange contributions. Furthermore, the electromagnetic coupling to the bound nucleon is dependent on the nuclear dynamics through the self-energy of the nucleon in the nuclear medium.

  18. Medium modifications with recoil polarization

    International Nuclear Information System (INIS)

    Brand, J.F.J. van den; Ent, R.

    1994-01-01

    The authors show that the virtual Compton scattering process allows for a precise study of the off-shell electron-nucleon vertex. In a separable model, they show the sensitivity to new unconstrained structure functions of the nucleon, beyond the usual Dirac and Pauli form factors. In addition, they show the sensitivity to bound nucleon form factors using the reaction 4He(rvec e,e',rvec p) 3 H. A nucleon embedded in a nucleus represents a complex system. Firstly, the bound nucleon is necessarily off-shell and in principle a complete understanding of the dynamical structure of the nucleon is required in order to calculate its off-shell electromagnetic interaction. Secondly, one faces the possibility of genuine medium effects, such as for example quark-exchange contributions. Furthermore, the electromagnetic coupling to the bound nucleon is dependent on the nuclear dynamics through the self-energy of the nucleon in the nuclear medium

  19. Medium Theory and Social Systems

    DEFF Research Database (Denmark)

    Tække, Jesper

      the  possibility  for  observation both of a social micro and a social macro level from a medium perspective. In the next  section  the paper  frames  the macro  level by  a  tentative  synthesis of  the medium  theory  and  the  sociological systems theory briefly describing a socio......-evolutionary process where new media alter  the societal capacity to handle complexity  in  time and space.  In  this section it becomes probable  that  by  means  of  different  media,  social  systems  give  different  possibilities  for  actual  social  performance.  In a way,  social  systems  themselves can be......  seen as medium  for  formation. Finally  the  paper  takes  the micro  level  perspective  by  applying  the  theory  to  newsgroups,  interpreting  them as self-organizing interactive systems giving a differentiated and diversified scope for social  inclusion.  ...

  20. Medium energy ion scattering (MEIS)

    International Nuclear Information System (INIS)

    Dittmann, K.; Markwitz, A.

    2009-01-01

    This report gives an overview about the technique and experimental study of medium energy ion scattering (MEIS) as a quantitative technique to determine and analyse the composition and geometrical structure of crystalline surfaces and near surface-layers by measuring the energy and yield of the backscattered ions. The use of a lower energy range of 50 to 500 keV accelerated ions impinging onto the target surface and the application of a high-resolution electrostatic energy analyser (ESA) makes medium energy ion scattering spectroscopy into a high depth resolution and surface-sensitive version of RBS with less resulting damage effects. This report details the first steps of research in that field of measurement technology using medium energetic backscattered ions detected by means of a semiconductor radiation detector instead of an ESA. The study of medium energy ion scattering (MEIS) has been performed using the 40 keV industrial ion implanter established at GNS Sciences remodelled with supplementary high voltage insulation for the ion source in order to apply voltages up to 45 kV, extra apertures installed in the beamline and sample chamber in order to set the beam diameter accurately, and a semiconductor radiation detector. For measurement purposes a beam of positive charged helium ions accelerated to an energy of about 80 keV has been used impinging onto target surfaces of lead implanted into silicon (PbSi), scandium implanted into aluminium (ScAl), aluminium foil (Al) and glassy carbon (C). First results show that it is possible to use the upgraded industrial implanter for medium energy ion scattering. The beam of 4 He 2+ with an energy up to 88 keV has been focussed to 1 mm in diameter. The 5 nA ion beam hit the samples under 2 x 10 -8 mbar. The results using the surface barrier detector show scattering events from the samples. Cooling of the detector to liquid nitrogen temperatures reduced the electronic noise in the backscattering spectrum close to zero. A

  1. Accretion from an inhomogeneous medium

    International Nuclear Information System (INIS)

    Livio, M.; Soker, N.; Koo, M. de; Savonije, G.J.

    1986-01-01

    The problem of accretion by a compact object from an inhomogeneous medium is studied in the general γnot=1 case. The mass accretion rate is found to decrease with increasing γ. The rate of accretion of angular momentum is found to be significantly lower than the rate at which angular momentum is deposited into the Bondi-Hoyle, symmetrical, accretion cylinder. The consequences of the results are studied for the cases of neutron stars accreting from the winds of early-type companions and white dwarfs and main-sequence stars accreting from winds of cool giants. (author)

  2. A Heterogeneous Medium Analytical Benchmark

    International Nuclear Information System (INIS)

    Ganapol, B.D.

    1999-01-01

    A benchmark, called benchmark BLUE, has been developed for one-group neutral particle (neutron or photon) transport in a one-dimensional sub-critical heterogeneous plane parallel medium with surface illumination. General anisotropic scattering is accommodated through the Green's Function Method (GFM). Numerical Fourier transform inversion is used to generate the required Green's functions which are kernels to coupled integral equations that give the exiting angular fluxes. The interior scalar flux is then obtained through quadrature. A compound iterative procedure for quadrature order and slab surface source convergence provides highly accurate benchmark qualities (4- to 5- places of accuracy) results

  3. Charmed hadrons in nuclear medium

    International Nuclear Information System (INIS)

    Tolos, L.; Gamermann, D.; Molina, R.; Nieves, J.; Oset, E.; Garcia-Recio, C.; Ramos, A.

    2010-01-01

    We study the properties of charmed hadrons in dense matter within a coupled-channel approach which accounts for Pauli blocking effects and meson self-energies in a self-consistent manner. We analyze the behaviour in this dense environment of dynamically-generated baryonic resonances as well as the open-charm meson spectral functions. We discuss the implications of the in-medium properties of open-charm mesons on the D s0 (2317) and the predicted X(3700) scalar resonances. (authors)

  4. Development of brachytherapy medium doserate

    International Nuclear Information System (INIS)

    Atang Susila; Ari Satmoko; Ahmad Rifai; Kristiyanti

    2010-01-01

    Brachytherapy has proven to be an effective treatment for different types of cancers and it become a common treatment modality in most radiotherapy clinics. PRPN has had experience in development of Low Dose Rate Brachytherapy for cervix cancer treatment. However the treatment process using LDR device needs 5 hours in time that the patient feel uncomfort. Therefore PRPN develops Medium Dose Rate Brachytherapy with radiation activity not more than 5 Currie. The project is divided into two stages. Purchasing of TPS software and TDS design are held in 2010, and the construction will be in 2011. (author)

  5. Medium modification of fragmentation functions

    International Nuclear Information System (INIS)

    Nezza, Pasquale Di

    2007-01-01

    Deep Inelastic Scattering is the cleanest process to investigate the space-time evolution of the hadronization. This was studied by the influence of the nuclear medium on lepto-production of hadrons at the Hermes experiment at DESY in semi-inclusive DIS of 27.6 GeV positrons off deuterium, nitrogen, krypton and xenon targets. The differential multiplicity for heavy targets relative to that of deuterium has been measured for the first time for various identified hadrons (φ + , φ - , φ 0 , k + , k - , p and anti-p) as a function of the virtual photon energy ?, the fraction z of this energy transferred to the hadron, and the hadron transverse momentum squared p 2 t . The distribution of the hadron transverse momentum is broadened towards high p 2 t in the nuclear medium, in a manner resembling the Cronin effect observed in collisions of heavy ions and protons with nuclei. The pt -broadening results give also important information about the pre-hadron formation time. Moreover, by studying the hadron attenuation of the leading and sub-leading hadrons, we report, for the first time, the possibility to better understand the hadron absorption and the energy loss contributions to the attenuation mechanism. (Author)

  6. Gravitational lensing in plasmic medium

    Energy Technology Data Exchange (ETDEWEB)

    Bisnovatyi-Kogan, G. S., E-mail: gkogan@iki.rssi.ru; Tsupko, O. Yu., E-mail: tsupko@iki.rssi.ru [Russian Academy of Sciences, Space Research Institute (Russian Federation)

    2015-07-15

    The influence of plasma on different effects of gravitational lensing is reviewed. Using the Hamiltonian approach for geometrical optics in a medium in the presence of gravity, an exact formula for the photon deflection angle by a black hole (or another body with a Schwarzschild metric) embedded in plasma with a spherically symmetric density distribution is derived. The deflection angle in this case is determined by the mutual combination of different factors: gravity, dispersion, and refraction. While the effects of deflection by the gravity in vacuum and the refractive deflection in a nonhomogeneous medium are well known, the new effect is that, in the case of a homogeneous plasma, in the absence of refractive deflection, the gravitational deflection differs from the vacuum deflection and depends on the photon frequency. In the presence of a plasma nonhomogeneity, the chromatic refractive deflection also occurs, so the presence of plasma always makes gravitational lensing chromatic. In particular, the presence of plasma leads to different angular positions of the same image if it is observed at different wavelengths. It is discussed in detail how to apply the presented formulas for the calculation of the deflection angle in different situations. Gravitational lensing in plasma beyond the weak deflection approximation is also considered.

  7. Unsaturated medium hydrocarbons pollution evaluation

    International Nuclear Information System (INIS)

    Di Luise, G.

    1991-01-01

    When the so called porous unsaturated medium, that's the vertical subsoil section between both the ground and water-table level, is interested by a hydrocarbons spill, the problem to evaluate the pollution becomes difficult: considering, essentially, the natural coexistence in it of two fluids, air and water, and the interactions between them. This paper reports that the problems tend to increase when a third fluid, the pollutant, immiscible with water, is introduced into the medium: a three-phases flow, which presents several analogies with the flow conditions present in an oil-reservoir, will be established. In such a situation, it would be very useful to handle the matter by the commonly used parameters in the oil reservoirs studies such as: residual saturation, relative permeability, phases mobility, to derive a first semiquantitative estimation of the pollution. The subsoil pollution form hydrocarbons agents is one of the worldwide more diffused causes of contamination: such events are generally referable to two main effects: accidental (oil pipeline breakdowns, e.g.), and continuous (underground tanks breaks, industrial plants leakages, e.g.)

  8. Is the intercloud medium pervasive

    International Nuclear Information System (INIS)

    Heiles, C.

    1980-01-01

    We consider the pervasiveness of the ''not strongly absorbing'' (NSA) H I gas, which is the intercloud medium in steady state theories of the interstellar medium. We study the question by analyzing wide emission components in nearby gas, and the absence of absorption components in distant gas. We conclude that the NSA material is deficient in the immediately local solar vicinity. In nearby regions it contains 38% of the interstellar H I; it is generally pervasive and often has internal motions which greatly increase its velocity dispersion above the 5 km s -1 minimum value. It contains large holes, perhaps ranging up to 400 pc diameter, which probably occupy 10--20% of the volume. In distant regions the NSA material seems to be pervasive outside 8 kpc galactic radius. For galactic radii between 8 and 10 kpc its thickness agrees with previous determinations of 370 pc for nearby regions. Outside 10 kpc the thickness increases dramatically. Inside 8 kpc there are no data

  9. How Does the Medium Affect the Message?

    Science.gov (United States)

    Dommermuth, William P.

    1974-01-01

    This experimental comparison of the advertising effectiveness of television, movies, radio, and print finds no support for McLuhan's idea that television is a "cool" medium and movies are a "hot" medium. (RB)

  10. 27 CFR 19.914 - Medium plants.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Medium plants. 19.914... OF THE TREASURY LIQUORS DISTILLED SPIRITS PLANTS Distilled Spirits For Fuel Use Permits § 19.914 Medium plants. Any person wishing to establish a medium plant shall make application for and obtain in...

  11. Mapping of moveout in a TTI medium

    KAUST Repository

    Stovas, A.; Alkhalifah, Tariq Ali

    2012-01-01

    To compute moveout in a transversely isotropic medium with tilted symmetry axis is a very complicated problem. We propose to split this problem into two parts. First, to compute the moveout in a corresponding VTI medium. Second, to map the computed moveout to a TTI medium.

  12. Medium-size nuclear plants

    International Nuclear Information System (INIS)

    Vogelweith, L.; Lavergne, J.C.; Martinot, G.; Weiss, A.

    1977-01-01

    CEA (TECHNICATOME) has developed a range of pressurized water reactors of the type ''CAS compact'' which are adapted to civil ship propulsion, or to electric power production, combined possibly with heat production, up to outputs equivalent to 125 MWe. Nuclear plants equipped with these reactors are suitable to medium-size electric networks. Among the possible realizations, two types of plants are mentioned as examples: 1) Floating electron-nuclear plants; and 2) Combined electric power and desalting plants. The report describes the design characteristics of the different parts of a 125 MWe unit floating electro-nuclear plant: nuclear steam system CAS 3 G, power generating plant, floating platform for the whole plant. The report gives attention to the different possibilities according to site conditions (the plant can be kept floating, in a natural or artificial basin, it can be put aground, ...) and to safety and environment factors. Such unit can be used in places where there is a growing demand in electric power and fresh water. The report describes how the reactor, the power generating plant and multiflash distillation units of an electric power-desalting plant can be combined: choice of the ratio water output/electric power output, thermal cycle combination, choice of the gain ratio, according to economic considerations, and to desired goal of water output. The report analyses also some technical options, such as: choice of the extraction point of steam used as heat supply of the desalting station (bleeding a condensation turbine, or recovering steam at the exhaust of a backpressure turbine), design making the system safe. Lastly, economic considerations are dealt with: combining the production of fresh water and electric power provides usually a much better energy balance and a lower cost for both products. Examples are given of some types of installations which combine medium-size reactors with fresh water stations yielding from 10000 to 120000 m 3 per day

  13. Tattoo: a multifaceted medium of communication

    Directory of Open Access Journals (Sweden)

    Christian Wymann

    2010-11-01

    Full Text Available This article suggests the systems theoretical distinction of form/medium as a useful tool for distinguishing social phenomena that might look as if they stem from the same process. This is shown to be the case for the tattoo and tattooing. The tattoo is conceived as a medium of communication through which different forms of communication emerge. Tattooing is one of these forms of communication that shapes the medium in a particular way. The current article sheds a special light on its intricate, communicational constellation, for which the concept of parallax is suggested. Law, medicine and cosmetics as other forms of communication use the medium of tattoo in their own way as well. The form/medium distinction allows us to grasp these different forms of communication, while it shows that they share the tattoo as medium. The article’s ultimate goal is to illustrate that the tattoo figures as a multifaceted medium of communication.

  14. Medium-Based Design: Extending a Medium to Create an Exploratory Learning Environment

    Science.gov (United States)

    Rick, Jochen; Lamberty, K. K.

    2005-01-01

    This article introduces "medium-based" design -- an approach to creating "exploratory learning environments" using the method of "extending a medium". First, the characteristics of exploratory learning environments and medium-based design are described and grounded in related work. Particular attention is given to "extending a medium" --…

  15. Dreams of a New Medium

    Directory of Open Access Journals (Sweden)

    Aden Evens

    2009-01-01

    Full Text Available Problematic at best, the desire for a transparent interface nevertheless drives much of digital culture and technology. But not the Web; or at least, not Web 1.0. Thoroughly commercialized, comfortably parsed into genres, serving billions of pages of predigested content to passive consumers, the World Wide Web as developed in the '90s unabashedly embraces its role as medium. While so many digital technologies work to hide their mediacy--drawing in the user with a total simulated sensorium, dematerializing the resistances of size and weight, untangling the knots of cables tying user to machine and machine to cubicle, minimizing the interface--Web 1.0 proudly clings to the browser as a glaring reminder of its medial character. While Web 2.0 has not forsaken the browser altogether, it nevertheless seems to offer a different sort of mediation. Arising alongside the atomization of browser functions, the ubiquitization of connectivity, and the coincidence of producer and user, Web 2.0 retains the form of a medium while reaching for the experiential logic of immediacy. This is not the immediacy of the transparent interface; rather, Web 2.0 effects an immediate relationship between the individual and culture. The interface does not disappear, but its mediacy is subsumed under the general form of cultural participation. Focusing on the "version upgrade" from Web 1.0 to 2.0, this essay will explore the implications for mediacy of this transition, noting that the fantasy of immediacy which drives Web 2.0 is layered and complex. The typical account of immediacy proposes to eliminate the interface and so construct a virtual reality (VR. But Web 2.0 mostly sidesteps the virtual, propelled instead by a fantasy of intuition in which the Web already knows what you want because it is you. Crucially, fantasies about the digital are effective: the computer's futurity inhabits our world, finding its expression in politics, advertising, budgeting, strategic planning

  16. Selection of culture medium and conditions for the production of ...

    African Journals Online (AJOL)

    defined medium–A, defined medium-B, synthetic medium, rich medium and industrial medium) showed that the synthetic medium yielded maximum yeast biomass (12.8 g/LDCW) followed by rich medium (11.7 g/L DCW) and defined medium B ...

  17. The interstellar medium in galaxies

    CERN Document Server

    1997-01-01

    It has been more than five decades ago that Henk van de Hulst predicted the observability of the 21-cm line of neutral hydrogen (HI ). Since then use of the 21-cm line has greatly improved our knowledge in many fields and has been used for galactic structure studies, studies of the interstellar medium (ISM) in the Milky Way and other galaxies, studies of the mass distribution of the Milky Way and other galaxies, studies of spiral struc­ ture, studies of high velocity gas in the Milky Way and other galaxies, for measuring distances using the Tully-Fisher relation etc. Regarding studies of the ISM, there have been a number of instrumen­ tal developments over the past decade: large CCD's became available on optical telescopes, radio synthesis offered sensitive imaging capabilities, not only in the classical 21-cm HI line but also in the mm-transitions of CO and other molecules, and X-ray imaging capabilities became available to measure the hot component of the ISM. These developments meant that Milky Way was n...

  18. Studies in medium energy physics

    International Nuclear Information System (INIS)

    Green, A.; Hoffmann, G.W.; McDonough, J.; Purcell, M.J.; Ray, R.L.; Read, D.E.; Worn, S.D.

    1991-12-01

    This document constitutes the (1991--1992) technical progress report and continuation proposal for the ongoing medium energy nuclear physics research program supported by the US Department of Energy through special Research Grant DE-FG05-88ER40444. The experiments discussed are conducted at the Los Alamos National Laboratory's (LANL) Clinton P. Anderson Meson Physics Facility (LAMPF) and the Alternating Gradient Synchrotron (AGS) facility of the Brookhaven National Laboratory (BNL). The overall motivation for the work discussed in this document is driven by three main objectives: (1) provide hadron-nucleon and hadron-nucleus scattering data which serve to facilitate the study of effective two-body interactions, test (and possibly determine) nuclear structure, and help study reaction mechanisms and dynamics; (2) provide unique, first-of-a-kind ''exploratory'' hadron-nucleus scattering data in the hope that such data will lead to discovery of new phenomena and new physics; and (3) perform precision tests of fundamental interactions, such as rare decay searches, whose observation would imply fundamental new physics

  19. Optical illusions induced by rotating medium

    Science.gov (United States)

    Zang, XiaoFei; Huang, PengCheng; Zhu, YiMing

    2018-03-01

    Different from the traditional single-function electromagnetic wave rotators (rotate the electromagnetic wavefronts), we propose that rotating medium can be extended to optical illusions such as breaking the diffraction limit and overlapping illusion. Furthermore, the homogeneous but anisotropic rotating medium is simplified by homogeneous and isotropic positive-index materials according to the effective medium theory, which is helpful for future device fabrication. Finite element simulations for the two-dimensional case are performed to demonstrate these properties.

  20. Effects of thyroid hormone supplementation on anastomotic healing after segmental colonic resection.

    Science.gov (United States)

    Karaman, Kerem; Bostanci, Erdal Birol; Dincer, Nazmiye; Ulas, Murat; Ozer, Ilter; Dalgic, Tahsin; Ercin, Ugur; Bilgihan, Ayse; Ginis, Zeynep; Akoglu, Musa

    2012-08-01

    Alterations of thyroid hormones in colorectal surgery were previously studied. The aim of the present study was to determine the effects of triiodothyronine (T3) supplementation on anastomotic healing after segmental colectomy. Thirty male Wistar albino rats were divided into sham (n = 6), control (n = 12), and experimental (n = 12) groups. Sham group rats were immediately sacrificed after segmental colonic resection. Control and experimental group rats underwent resection and anastomosis. Experimental group rats received a single dose of T3 (400 μg/100 g) in postoperative day 1. Half of both control and experimental group rats were sacrificed on postoperative d 3 and the remaining half were sacrificed on postoperative d 7. Hydroxiproline (HP), myeloperoxidase (MPO), thyroid stimulating hormone (TSH), free T3 (FT3), and free thyroxine (FT4) levels, bursting pressure, and histologic analyses of the anastomotic segments were compared. FT3 levels significantly decreased in control groups rats compared with the sham group (P < 0.01). However, T3 hormone given rats had no decline in FT3 levels. Anastomotic bursting pressure was significantly higher in the experimental group rats on postoperative d 7 (P = 0.015). Histopathologic analyses of the anastomotic segments determined significantly more severe edema and necrosis in control group rats (P < 0.05). Collagen deposition in the anastomotic tissue was significantly higher in experimental group rats on postoperative d 7 (P = 0.015). Anastomosis after colon resection is associated with decreased FT3 level. T3 supplementation ameliorates the reduction in FT3 and seems to provide constructive therapeutic effects on anastomotic healing. Copyright © 2012 Elsevier Inc. All rights reserved.

  1. Disrupted fat distribution and composition due to medium-chain triglycerides in mice with a β-oxidation defect.

    Science.gov (United States)

    Tucci, Sara; Flögel, Ulrich; Sturm, Marga; Borsch, Elena; Spiekerkoetter, Ute

    2011-08-01

    Because of the enhanced recognition of inherited long-chain fatty acid oxidation disorders by worldwide newborn screening programs, an increasing number of asymptomatic patients receive medium-chain triglyceride (MCT) supplements to prevent the development of cardiomyopathy and myopathy. MCT supplementation has been recognized as a safe dietary intervention, but long-term observations into later adulthood are still not available. We investigated the consequences of a prolonged MCT diet on abdominal fat distribution and composition and on liver fat. Mice with very-long-chain acyl-coenzyme A dehydrogenase deficiency (VLCAD(-/-)) were supplemented for 1 y with a diet in which MCTs replaced long-chain triglycerides without increasing the total fat content. The dietary effects on abdominal fat accumulation and composition were analyzed by in vivo (1)H- and (13)C-magnetic resonance spectroscopy (9.4 Tesla). After 1 y of MCT supplementation, VLCAD(-/-) mice accumulated massive visceral fat and had a dramatic increase in the concentration of serum free fatty acids. Furthermore, we observed a profound shift in body triglyceride composition, ie, concentrations of physiologically important polyunsaturated fatty acids dramatically decreased. (1)H-Magnetic resonance spectroscopy analysis and histologic evaluation of the liver also showed pronounced fat accumulation and marked oxidative stress. Although the MCT-supplemented diet has been reported to prevent the development of cardiomyopathy and skeletal myopathy in fatty acid oxidation disorders, our data show that long-term MCT supplementation results in a severe clinical phenotype similar to that of nonalcoholic steatohepatitis and the metabolic syndrome.

  2. Recordable storage medium with protected data area

    NARCIS (Netherlands)

    2005-01-01

    The invention relates to a method of storing data on a rewritable data storage medium, to a corresponding storage medium, to a corresponding recording apparatus and to a corresponding playback apparatus. Copy-protective measures require that on rewritable storage media some data must be stored which

  3. Electromagnetic Sources in a Moving Conducting Medium

    DEFF Research Database (Denmark)

    Johannsen, Günther

    1971-01-01

    The problem of an arbitrary source distribution in a uniformly moving, homogeneous, isotropic, nondispersive, conducting medium is solved. The technique used is to solve the problem in the rest system of the medium and then write the result in an appropriate four-dimensional, covariant form which...

  4. Kultivasi Scenedesmus SP. Pada Medium Air Limbah

    OpenAIRE

    Kawaroe, Mujizat

    2011-01-01

    Proses fotosintesis pada mikroalga membutuhkan CO2 dan cahaya matahari serta nutrien untuk pertumbuhannya. Kultivasi Scenedesmus sp. pada medium air limbah bertujuan guna mencukupi kebutuhan mikroalga akan nutrien dan mengurangi masukan dari bahan kimia yang terkandung dalam air limbah tersebut ke lingkungan. Kultivasi Scenedesmus sp. dilakukan selama tujuh hari pada medium air limbah industri tanpa penambahan nutri...

  5. Effective medium theory for anisotropic metamaterials

    KAUST Repository

    Zhang, Xiujuan; Wu, Ying

    2015-01-01

    -dimensional metamaterial composed of a rectangular array of elliptic cylinders and derive an effective medium theory for such a metamaterial. We find that it is possible to obtain a closed-form analytical solution for the anisotropic effective medium parameters, provided

  6. Selective medium for aerobic incubation of Campylobacter

    Science.gov (United States)

    Studies were conducted on the formulation of a selective medium that could be used to isolate Campylobacter from mixed bacterial cultures using aerobic incubation. A non-selective, basal broth medium was prepared and supplemented with Bolton, Cefex, or Skirrow antibiotic mixtures. The ability of pur...

  7. Effective medium theory for anisotropic metamaterials

    KAUST Repository

    Zhang, Xiujuan

    2015-01-20

    Materials with anisotropic material parameters can be utilized to fabricate many fascinating devices, such as hyperlenses, metasolids, and one-way waveguides. In this study, we analyze the effects of geometric anisotropy on a two-dimensional metamaterial composed of a rectangular array of elliptic cylinders and derive an effective medium theory for such a metamaterial. We find that it is possible to obtain a closed-form analytical solution for the anisotropic effective medium parameters, provided the aspect ratio of the lattice and the eccentricity of the elliptic cylinder satisfy certain conditions. The derived effective medium theory not only recovers the well-known Maxwell-Garnett results in the quasi-static regime, but is also valid beyond the long-wavelength limit, where the wavelength in the host medium is comparable to the size of the lattice so that previous anisotropic effective medium theories fail. Such an advance greatly broadens the applicable realm of the effective medium theory and introduces many possibilities in the design of structures with desired anisotropic material characteristics. A real sample of a recently theoretically proposed anisotropic medium, with a near-zero index to control the flux, is achieved using the derived effective medium theory, and control of the electromagnetic waves in the sample is clearly demonstrated.

  8. Micropropagation of Alstroemeria in liquid medium using slow release of medium components

    NARCIS (Netherlands)

    Klerk, de G.J.M.; Brugge, ter J.

    2010-01-01

    Alstroemeria rhizomes were micropropagated on semi-solid medium (AM) and in liquid medium (LM). In LM, growth was much enhanced (ca. 70%). Adequate gas exchange was crucial. This was obtained by agitation and in static medium by a sufficient large contact area of the explant and the gaseous

  9. Collaborative Manufacturing for Small-Medium Enterprises

    Science.gov (United States)

    Irianto, D.

    2016-02-01

    Manufacturing systems involve decisions concerning production processes, capacity, planning, and control. In a MTO manufacturing systems, strategic decisions concerning fulfilment of customer requirement, manufacturing cost, and due date of delivery are the most important. In order to accelerate the decision making process, research on decision making structure when receiving order and sequencing activities under limited capacity is required. An effective decision making process is typically required by small-medium components and tools maker as supporting industries to large industries. On one side, metal small-medium enterprises are expected to produce parts, components or tools (i.e. jigs, fixture, mold, and dies) with high precision, low cost, and exact delivery time. On the other side, a metal small- medium enterprise may have weak bargaining position due to aspects such as low production capacity, limited budget for material procurement, and limited high precision machine and equipment. Instead of receiving order exclusively, a small-medium enterprise can collaborate with other small-medium enterprise in order to fulfill requirements high quality, low manufacturing cost, and just in time delivery. Small-medium enterprises can share their best capabilities to form effective supporting industries. Independent body such as community service at university can take a role as a collaboration manager. The Laboratory of Production Systems at Bandung Institute of Technology has implemented shared manufacturing systems for small-medium enterprise collaboration.

  10. Photonic-resonant left-handed medium

    International Nuclear Information System (INIS)

    Shen Jianqi

    2006-01-01

    A new scheme to realize simultaneously negative permittivity and permeability in a coherent atomic vapor medium (photonic-resonant material) via a coherent driving mechanism is suggested. It is verified that the atomic system coherently driven by a strong optical field will give rise to a negative refractive index in certain probe frequency ranges. One of the most remarkable features of the present scheme is such that a slab fabricated by the left-handed vapor medium is an ideal candidate for designing perfect lenses since the photonic-resonant atomic vapor cannot only exhibit an isotropic negative refractive index, but also provide a good impedance match at the air-medium interfaces

  11. Physical processes in the interstellar medium

    CERN Document Server

    Spitzer, Lyman

    2008-01-01

    Physical Processes in the Interstellar Medium discusses the nature of interstellar matter, with a strong emphasis on basic physical principles, and summarizes the present state of knowledge about the interstellar medium by providing the latest observational data. Physics and chemistry of the interstellar medium are treated, with frequent references to observational results. The overall equilibrium and dynamical state of the interstellar gas are described, with discussions of explosions produced by star birth and star death and the initial phases of cloud collapse leading to star formation.

  12. Optimization of medium composition for apple rootstocks

    African Journals Online (AJOL)

    User

    2011-05-02

    May 2, 2011 ... Key words: Apple rootstocks, medium composition, multiplication rate, plant growth regulators (PGRs). ... be extrapolated with the same success for another .... Analysis System (SAS) software program (SAS Institute Inc. 1999).

  13. Jet multiplicity distributions: medium dependence in MLLA

    International Nuclear Information System (INIS)

    Armesto, Nestor; Pajares, Carlos; Quiroga-Arias, Paloma

    2009-01-01

    We study the medium dependence of the multiplicity distributions in the modified leading logarithmic approximation. We focus in the enhancement in the number of branchings as the partons travel trough a dense medium created in a heavy-ion collision. We study the effect of a higher number of splittings in some jet observables by introducing the medium as a constant (f med ) in the splitting functions. Having as our ansatz for the quark and gluon jets mean multiplicities left angle n G right angle =e γy and left angle n Q right angle =r -1 e γy , we study in an analytic approach the dependence with the medium (f med ) of the anomalous dimension (γ), the multiplicity ratio (r), and so the mean multiplicities. We also obtain the higher-order moments of the multiplicity distribution, what allows us to study its dispersion. (orig.)

  14. Optimization of medium composition for thermostable protease ...

    African Journals Online (AJOL)

    SERVER

    2008-04-17

    Apr 17, 2008 ... Optimization of the fermentation medium for maximization of thermostable neutral protease production by Bacillus sp. ..... Each contour curve represented an infinite number of combinations of two ..... Production in sea-water of.

  15. Fractional diffusion equation for heterogeneous medium

    International Nuclear Information System (INIS)

    Polo L, M. A.; Espinosa M, E. G.; Espinosa P, G.; Del Valle G, E.

    2011-11-01

    The asymptotic diffusion approximation for the Boltzmann (transport) equation was developed in 1950 decade in order to describe the diffusion of a particle in an isotropic medium, considers that the particles have a diffusion infinite velocity. In this work is developed a new approximation where is considered that the particles have a finite velocity, with this model is possible to describe the behavior in an anomalous medium. According with these ideas the model was obtained from the Fick law, where is considered that the temporal term of the current vector is not negligible. As a result the diffusion equation of fractional order which describes the dispersion of particles in a highly heterogeneous or disturbed medium is obtained, i.e., in a general medium. (Author)

  16. Small and medium power reactors 1987

    International Nuclear Information System (INIS)

    1987-12-01

    This TECDOC follows the publication of TECDOC-347 Small and Medium Power Reactors Project Initiation Study - Phase I published in 1985 and TECDOC-376 Small and Medium Power Reactors 1985 published in 1986. It is mainly intended for decision makers in Developing Member States interested in embarking on a nuclear power programme. It consists of two parts: 1) Guidelines for the Introduction of Small and Medium Power Reactors in Developing Countries. These Guidelines were established during the Advisory Group Meeting held in Vienna from 11 to 15 May 1987. Their purpose is to review key aspects relating to the introduction of Small and Medium Power Reactors in developing countries; 2) Up-dated Information on SMPR Concepts Contributed by Supplier Industries. According to the recommendations of the Second Technical Committee Meeting on SMPRs held in Vienna in March 1985, this part contains the up-dated information formerly published in Annex I of the above mentioned TECDOC-347. Figs

  17. Incompatibility of Contrast Medium and Trisodium Citrate

    International Nuclear Information System (INIS)

    Delcour, Christian; Bruninx, Guy

    2013-01-01

    To test the compatibility of trisodium citrate, a catheter lock solution, with iodinated contrast medium. Iohexol, iobitridol, iodixanol, ioxaglate, ioxithalamate, iomeprol, and iopromide were tested. In all tests, 2 ml of contrast medium were mixed with 2 ml of trisodium citrate solution. Iodixanol and ioxaglate provoked a highly viscous gluelike precipitation when mixed with trisodium citrate. A brief transient precipitate was observed with iohexol, iomeprol, and ioxithalamate. Permanent precipitation occurred with iobitridol and iopromide. One must be aware of the potential for precipitation when contrast medium is mixed with trisodium citrate solution. Before trisodium citrate solution is injected, the catheter should be thoroughly flushed with saline if a contrast medium has previously been injected through it.

  18. Study of niobium corrosion in alkaline medium

    International Nuclear Information System (INIS)

    Almeida, S.H. de.

    1987-01-01

    A comparative study of niobium electrochemical behaviour in NaOH and KOH solution, with concentrations between 0,5 and 6,1M is presented. The studies were done through electrochemicals assays, consisting in the corrosion potential and anodic and cathodic polarization curves, complemented by loss of mass experiments. The niobium anodic behaviour in alkaline medium is characterized by passivation occurrence, with a stable film formation. The Na oH solution in alkaline medium are more corrosible to niobium than the KOH solution. The loss of mass assays showed that the corrosion velocit is more dependente of hydroxide concentration in KOH medium than the NaOH medium. (C.G.C.) [pt

  19. Small and Medium Enterprises and Biopharmaceutical Innovations ...

    African Journals Online (AJOL)

    Erah

    Benin City, 300001 Nigeria. All rights ... are challenges facing African Small and Medium Enterprises (SMEs) in biopharmaceutical industry, the ... Network for Drug and Diagnostics recognizes .... functionality is in place, integration into the.

  20. Sharing perspectives on English-medium instruction

    CERN Document Server

    Ackerley, Katherine; Helm, Francesca

    2017-01-01

    This volume gives voice to the views and experiences of researchers, lecturers, administrative staff, teacher trainers and students with regard to the implementation of English-medium instruction in a public university based in the north-east of Italy.

  1. Medium Effects in Reactions with Rare Isotopes

    International Nuclear Information System (INIS)

    Bertulani, C A; Karakoç, M

    2012-01-01

    We discuss medium effects in knockout reactions with rare isotopes of weakly-bound nuclei at intermediate energies. We show that the poorly known corrections may lead to sizable modifications of knockout cross sections and momentum dsitributions.

  2. Effective medium theory principles and applications

    CERN Document Server

    Choy, Tuck C

    2015-01-01

    Effective medium theory dates back to the early days of the theory of electricity. Faraday in 1837 proposed one of the earliest models for a composite metal-insulator dielectric and around 1870 Maxwell and later Garnett (1904) developed models to describe a composite or mixed material medium. The subject has been developed considerably since and while the results are useful for predicting materials performance, the theory can also be used in a wide range of problems in physics and materials engineering. This book develops the topic of effective medium theory by bringing together the essentials of both the static and the dynamical theory. Electromagnetic systems are thoroughly dealt with, as well as related areas such as the CPA theory of alloys, liquids, the density functional theory etc., with applications to ultrasonics, hydrodynamics, superconductors, porous media and others, where the unifying aspects of the effective medium concept are emphasized. In this new second edition two further chapters have been...

  3. Small and medium power reactors 1987

    Science.gov (United States)

    1987-12-01

    This TECDOC follows the publication of TECDOC-347: Small and Medium Power Reactors (SMPR) Project Initiation Study, Phase 1, published in 1985 and TECDOC-376: Small and Medium Power Reactors 1985 published in 1986. It is mainly intended for decision makers in Developing Member States interested in embarking on a nuclear power program. It consists of two parts: (1) guidelines for the introduction of small and medium power reactors in developing countries. These Guidelines were established during the Advisory Group Meeting held in Vienna from 11 to 15 May 1987. Their purpose is to review key aspects relating to the introduction of small and medium power reactors in developing countries; (2) up-dated information on SMPR Concepts Contributed by Supplier Industries. According to the recommendations of the Second Technical Committee Meeting on SMPRs held in Vienna in March 1985, this part contains the up-dated information formerly published in Annex 1 of the above mentioned TECDOC-347.

  4. Switching power converters medium and high power

    CERN Document Server

    Neacsu, Dorin O

    2013-01-01

    An examination of all of the multidisciplinary aspects of medium- and high-power converter systems, including basic power electronics, digital control and hardware, sensors, analog preprocessing of signals, protection devices and fault management, and pulse-width-modulation (PWM) algorithms, Switching Power Converters: Medium and High Power, Second Edition discusses the actual use of industrial technology and its related subassemblies and components, covering facets of implementation otherwise overlooked by theoretical textbooks. The updated Second Edition contains many new figures, as well as

  5. DYNAMIC DEFORMATION THE VISCOELASTIC TWOCOMPONENT MEDIUM

    Directory of Open Access Journals (Sweden)

    V. S. Polenov

    2015-01-01

    Full Text Available Summary. In the article are scope harmonious warping of the two-component medium, one component which are represent viscoelastic medium, hereditary properties which are described by the kernel aftereffect Abel integral-differential ratio BoltzmannVolterr, while second – compressible liquid. Do a study one-dimensional case. Use motion equation of two-component medium at movement. Look determination system these equalization in the form of damped wave. Introduce dimensionless coefficient. Combined equations happen to homogeneous system with complex factor relatively waves amplitude in viscoelastic component and in fluid. As a result opening system determinant receive biquadratic equation. Elastic operator express through kernel aftereffect Abel for space Fourier. With the help transformation and symbol series biquadratic equation reduce to quadratic equation. Come to the conclusion that in two-component viscoelastic medium exist two mode sonic waves. As a result solution of quadratic equation be found description advance of waves sonic in viscoelastic two-component medium, which physical-mechanical properties represent complex parameter. Velocity determination advance of sonic waves, attenuation coefficient, mechanical loss tangent, depending on characteristic porous medium and circular frequency formulas receive. Graph dependences of description advance of waves sonic from the temperature logarithm and with the fractional parameter γ are constructed.

  6. Selective medium for culture of Mycoplasma hyopneumoniae.

    Science.gov (United States)

    Cook, Beth S; Beddow, Jessica G; Manso-Silván, Lucía; Maglennon, Gareth A; Rycroft, Andrew N

    2016-11-15

    The fastidious porcine respiratory pathogen Mycoplasma hyopneumoniae has proven difficult to culture since it was first isolated in 1965. A reliable solid medium has been particularly challenging. Moreover, clinical and pathological samples often contain the fast-growing M. hyorhinis which contaminates and overgrows M. hyopneumoniae in primary culture. The aim of this study was to optimise the culture medium for recovery of M. hyopneumoniae and to devise a medium for selection of M. hyopneumoniae from clinical samples also containing M. hyorhinis. The solid medium devised by Niels Friis was improved by use of Purified agar and incorporation of DEAE-dextran. Addition of glucose or neutralization of acidity in liquid medium with NaOH did not improve the final yield of viable organisms or alter the timing of peak viability. Analysis of the relative susceptibility of M. hyopneumoniae and M. hyorhinis strains to four antimicrobials showed that M. hyopneumoniae is less susceptible than M. hyorhinis to kanamycin. This was consistent in all UK and Danish strains tested. A concentration of 2μg/ml of kanamycin selectively inhibited the growth of all M. hyorhinis tested, while M. hyopneumoniae was able to grow. This forms the basis of an effective selective culture medium for M. hyopneumoniae. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  7. Overview of medium heterogeneity and transport processes

    International Nuclear Information System (INIS)

    Tsang, Y.; Tsang, C.F.

    1993-11-01

    Medium heterogeneity can have significant impact on the behavior of solute transport. Tracer breakthrough curves from transport in a heterogeneous medium are distinctly different from that in a homogeneous porous medium. Usually the shape of the breakthrough curves are highly non-symmetrical with a fast rise at early times and very long tail at late times, and often, they consist of multiple peaks. Moreover, unlike transport in a homogeneous medium where the same transport parameters describe the entire medium, transport through heterogeneous media gives rise to breakthrough curves which have strong spatial dependence. These inherent characteristics of transport in heterogeneous medium present special challenge to the performance assessment of a potential high level nuclear waste repository with respect to the possible release of radio nuclides to the accessible environment. Since an inherently desirable site characteristic for a waste repository is that flow and transport should be slow, then transport measurements in site characterization efforts will necessarily be spatially small and temporally short compare to the scales which are of relevance to performance assessment predictions. In this paper we discuss the role of medium heterogeneity in site characterization and performance assessment. Our discussion will be based on a specific example of a 3D heterogeneous stochastic model of a site generally similar to, the Aespoe Island, the site of the Hard Rock Laboratory in Southern Sweden. For our study, alternative 3D stochastic fields of hydraulic conductivities conditioned on ''point'' measurements shall be generated. Results of stochastic flow and transport simulations would be used to address the issues of (1) the relationship of tracer breakthrough with the structure of heterogeneity, and (2) the inference from small scale testing results to large scale and long term predictions

  8. Evaluation of Insulin Medium or Chondrogenic Medium on Proliferation and Chondrogenesis of ATDC5 Cells

    OpenAIRE

    Yao, Yongchang; Zhai, Zhichen; Wang, Yingjun

    2014-01-01

    Background. The ATDC5 cell line is regarded as an excellent cell model for chondrogenesis. In most studies with ATDC5 cells, insulin medium (IM) was used to induce chondrogenesis while chondrogenic medium (CM), which was usually applied in chondrogenesis of mesenchymal stem cells (MSCs), was rarely used for ATDC5 cells. This study was mainly designed to investigate the effect of IM, CM, and growth medium (GM) on chondrogenesis of ATDC5 cells. Methods. ATDC5 cells were, respectively, cultured ...

  9. 'That proves my point': How mediums reconstrue disconfirmation in medium-sitter interactions.

    Science.gov (United States)

    Enoksen, Anette Einan; Dickerson, Paul

    2018-04-01

    Previous research has examined how the talk of mediums attends to the epistemological status of their readings. Such work has identified that mediums frequently use question-framed propositions that are typically confirmed by the sitter, thereby conferring epistemological status on the medium. This study seeks to investigate what happens when the sitter disconfirms the propositions of the medium. The study focuses on the ways in which such disconfirmation can be responded to such that it is reconstrued as evidence of the psychic nature of the medium's reading. Televised demonstrations of psychic readings involving British and US mediums and their sitters are analysed. The results suggest that mediums rework disconfirmation as proof in several ways: first, by emphasizing the different access that sitter and medium have to knowledge (e.g., about the future); second, as evidence that the medium has access to the actual voice of the deceased (and may therefore mishear what the deceased has said to them); and third, as revealing an important truth that has hitherto been concealed from the sitter. The implications of these findings are considered for cases where speakers bring different and potentially competing, epistemological resources to an interaction. © 2018 The British Psychological Society.

  10. CHROMagar Orientation Medium Reduces Urine Culture Workload

    Science.gov (United States)

    Manickam, Kanchana; Karlowsky, James A.; Adam, Heather; Lagacé-Wiens, Philippe R. S.; Rendina, Assunta; Pang, Paulette; Murray, Brenda-Lee

    2013-01-01

    Microbiology laboratories continually strive to streamline and improve their urine culture algorithms because of the high volumes of urine specimens they receive and the modest numbers of those specimens that are ultimately considered clinically significant. In the current study, we quantitatively measured the impact of the introduction of CHROMagar Orientation (CO) medium into routine use in two hospital laboratories and compared it to conventional culture on blood and MacConkey agars. Based on data extracted from our Laboratory Information System from 2006 to 2011, the use of CO medium resulted in a 28% reduction in workload for additional procedures such as Gram stains, subcultures, identification panels, agglutination tests, and biochemical tests. The average number of workload units (one workload unit equals 1 min of hands-on labor) per urine specimen was significantly reduced (P < 0.0001; 95% confidence interval [CI], 0.5326 to 1.047) from 2.67 in 2006 (preimplementation of CO medium) to 1.88 in 2011 (postimplementation of CO medium). We conclude that the use of CO medium streamlined the urine culture process and increased bench throughput by reducing both workload and turnaround time in our laboratories. PMID:23363839

  11. Hadrons in hot and dense medium

    International Nuclear Information System (INIS)

    Mallik, S.

    2004-01-01

    We review chiral perturbation theory in some detail and construct interaction terms involving the Goldstone and the different non-Goldstone fields, in presence of external (classical) fields coupled to currents. The ensemble average of the two-point functions of the currents can now be expanded in terms of Feynman diagrams. We evaluate the one-loop diagrams in the neighbourhood of the respective poles to find the effective couplings and masses of the particles in medium. We also describe the virial formula for the self-energy of a particle in medium, giving its pole position. It proves useful if the scattering amplitude of the particle with particles in medium is known experimentally. (author)

  12. Charmonium propagation through a dense medium

    Directory of Open Access Journals (Sweden)

    Kopeliovich B.Z.

    2015-01-01

    Full Text Available Attenuation of a colourless c̄c dipole propagating with a large momentum through a hot medium originates from two sources, Debye screening (melting, and inelastic collisions with surrounding scattering centres (absorption. The former never terminates completely production of a bound charmonium in heavy ion collisions, even at very high temperatures. The latter, is controlled my the magnitude of the dipole cross section, related to the transport coefficient, which is the rate of transverse momentum broadening in the medium. A novel procedure of Lorentz boosting of the Schrödinger equation is developed, which allows to calculate the charmonium survival probability employing the path-integral technique, incorporating both melting and absorption. A novel mechanism of charmonium regeneration in a dense medium is proposed.

  13. Coherent neutrino interactions in a dense medium

    International Nuclear Information System (INIS)

    Kiers, K.; Weiss, N.

    1997-01-01

    Motivated by the effect of matter on neutrino oscillations (the MSW effect) we study in more detail the propagation of neutrinos in a dense medium. The dispersion relation for massive neutrinos in a medium is known to have a minimum at nonzero momentum p∼G F ρ/√(2). We study in detail the origin and consequences of this dispersion relation for both Dirac and Majorana neutrinos both in a toy model with only neutral currents and a single neutrino flavor and in a realistic open-quotes standard modelclose quotes with two neutrino flavors. We find that for a range of neutrino momenta near the minimum of the dispersion relation, Dirac neutrinos are trapped by their coherent interactions with the medium. This effect does not lead to the trapping of Majorana neutrinos. copyright 1997 The American Physical Society

  14. Temperature distribution in a uniformly moving medium

    International Nuclear Information System (INIS)

    Mitchell, Joseph D; Petrov, Nikola P

    2009-01-01

    We apply several physical ideas to determine the steady temperature distribution in a medium moving with uniform velocity between two infinite parallel plates. We compute it in the coordinate frame moving with the medium by integration over the 'past' to account for the influence of an infinite set of instantaneous point sources of heat in past moments as seen by an observer moving with the medium. The boundary heat flux is simulated by appropriately distributed point heat sources on the inner side of an adiabatically insulating boundary. We make an extensive use of the Green functions with an emphasis on their physical meaning. The methodology used in this paper is of great pedagogical value as it offers an opportunity for students to see the connection between powerful mathematical techniques and their physical interpretation in an intuitively clear physical problem. We suggest several problems and a challenging project that can be easily incorporated in undergraduate or graduate courses

  15. Graphene wire medium: Homogenization and application

    DEFF Research Database (Denmark)

    Andryieuski, Andrei; Chigrin, Dmitry N.; Lavrinenko, Andrei

    2012-01-01

    In this contribution we analyze numerically the optical properties of the graphene wire medium, which unit cell consists of a stripe of graphene embedded into dielectric. We propose a simple method for retrieval of the isofrequency contour and effective permittivity tensor. As an example of the g......In this contribution we analyze numerically the optical properties of the graphene wire medium, which unit cell consists of a stripe of graphene embedded into dielectric. We propose a simple method for retrieval of the isofrequency contour and effective permittivity tensor. As an example...... of the graphene wire medium application we demonstrate a reconfigurable hyperlens for the terahertz subwavelength imaging capable of resolving two sources with separation λ0/5 in the far-field....

  16. Small and medium size nuclear reactors

    International Nuclear Information System (INIS)

    Al-Mugrabi, M.A.

    1996-01-01

    The purpose of this appendix is to provide up-to-date technical information relevant to the deployment of small and medium reactors (SMRs). It summarizes the status of SMRs and discusses areas of relevance to their utilization, including seawater desalination; and in particular their simplicity, their flexibility for a variety of applications and the use of passive safety features as fundamental to most of these designs. In response to important commercial developments, the energy range of small and medium reactors is now taken as being up to around 700 MW(e). Detailed information on SMR designs can be found in the IAEA report on The Design and Development Status of Small and Medium Reactor Systems 1995. 5 refs, 2 figs, 1 tab

  17. H2 molecules and the intercloud medium

    International Nuclear Information System (INIS)

    Hill, J.K.; Hollenbach, D.J.

    1976-01-01

    We discuss expected column of densities of H 2 in the intercloud medium and the possible use of molecules as indicators of intercloud physical conditions. We treat molecule formation by the H - process and on graphite grains and show that the Barlow-Silk hypothesis of a 1 eV semichemical hydrogen-graphite bond leads to a large enhancement of the intercloud molecule formation rate. Rotational excitation calculations are presented for both cloud and intercloud conditions which show, in agreement with Jura, that the presently observed optically thin H 2 absorption components are more likely to originate in cold clouds than in the intercloud medium

  18. Medium for Children’s Creativity

    DEFF Research Database (Denmark)

    Borum, Nanna; Kristensen, Kasper; Petersson, Eva

    2014-01-01

    This paper reports on an exploratory study that investigates 16 elementary school children’s interaction with two different mediums for creativity, LEGO® bricks and paper collages, drawing on the previous creativity assessment test carried out by Amabile [1]. The study is based in a playful...... on labor when working with LEGO bricks. It was also evident that the children assigned preconceived affordances to the two mediums. The results from this study should feed into to a technology enhanced playful learning environment and these are the initial steps in the design process....

  19. Heavy quark energy loss in nuclear medium

    International Nuclear Information System (INIS)

    Zhang, Benr-Wei; Wang, Enke; Wang, Xin-Nian

    2003-01-01

    Multiple scattering, modified fragmentation functions and radiative energy loss of a heavy quark propagating in a nuclear medium are investigated in perturbative QCD. Because of the quark mass dependence of the gluon formation time, the medium size dependence of heavy quark energy loss is found to change from a linear to a quadratic form when the initial energy and momentum scale are increased relative to the quark mass. The radiative energy loss is also significantly suppressed relative to a light quark due to the suppression of collinear gluon emission by a heavy quark

  20. Power converters for medium voltage networks

    CERN Document Server

    Islam, Md Rabiul; Zhu, Jianguo

    2014-01-01

    This book examines a number of topics, mainly in connection with advances in semiconductor devices and magnetic materials and developments in medium and large-scale renewable power plant technologies, grid integration techniques and new converter topologies, including advanced digital control systems for medium-voltage networks. The book's individual chapters provide an extensive compilation of fundamental theories and in-depth information on current research and development trends, while also exploring new approaches to overcoming some critical limitations of conventional grid integration te

  1. Structure and evolution of the interstellar medium

    International Nuclear Information System (INIS)

    Chieze, J.P.

    1985-10-01

    We give a two dimensional hydrodynamical analysis of HI clouds collisions in order to determine the mass spectrum of diffuse interstellar clouds. We have taken into account evaporation and abrasion by supernovae blast waves. The conditions for cloud merging or fragmentation are precised. Applications to the model of the interstellar medium of Mc Kee and Ostriker are also discussed. On the other hand, we show that molecular clouds belong to a one parameter family which can be identified to the sequence of the gravitationally unstable states of clouds bounded by the uniform pressure of the coronal phase of the interstellar medium. Hierarchical fragmentation of molecular clouds is analysed in this context [fr

  2. Optimizing culture medium for debittering constitutive enzyme ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-08-02

    Aug 2, 2010 ... naringinase on different matrices has been studied by many researchers (Busto et ... 10 g/L in the base medium compared to naringin control. Nitrogen ... Fermentation experiments were carried out in shaking flask for 5 days at 28°C with initial pH 6.0. † Values ..... fujikuroi mycelium in fluidized bioreactors.

  3. Jet multiplicity distributions: medium dependence in MLLA

    Energy Technology Data Exchange (ETDEWEB)

    Armesto, Nestor; Pajares, Carlos; Quiroga-Arias, Paloma [Universidade de Santiago de Compostela, Departamento de Fisica de Particulas and IGFAE, Santiago de Compostela (Spain)

    2009-07-15

    We study the medium dependence of the multiplicity distributions in the modified leading logarithmic approximation. We focus in the enhancement in the number of branchings as the partons travel trough a dense medium created in a heavy-ion collision. We study the effect of a higher number of splittings in some jet observables by introducing the medium as a constant (f{sub med}) in the splitting functions. Having as our ansatz for the quark and gluon jets mean multiplicities left angle n{sub G} right angle =e{sup {gamma}}{sup y} and left angle n{sub Q} right angle =r{sup -1}e{sup {gamma}}{sup y}, we study in an analytic approach the dependence with the medium (f{sub med}) of the anomalous dimension ({gamma}), the multiplicity ratio (r), and so the mean multiplicities. We also obtain the higher-order moments of the multiplicity distribution, what allows us to study its dispersion. (orig.)

  4. Wave propagation in thermoelastic saturated porous medium

    Indian Academy of Sciences (India)

    the existence and propagation of four waves in the medium. Three of the waves are ... predicted infinite speed for propagation of ther- mal signals. Lord and ..... saturated reservoir rock (North-sea Sandstone) is chosen for the numerical model ...

  5. Innovation in Small and Medium Firms.

    Science.gov (United States)

    Organisation for Economic Cooperation and Development, Paris (France). Committee for Scientific and Technical Personnel.

    A study analyzed the policies of 20 Organisation for Economic Cooperation and Development (OECD) member countries pertaining to small and medium enterprises (SMEs). Aims of the study were to assess the current role of SMEs as generators and users of innovations, to analyze the components of a favorable climate for innovation in SMEs, and to…

  6. The MIRI Medium Resolution Spectrometer calibration pipeline

    NARCIS (Netherlands)

    Labiano, A.; Azzollini, R.; Bailey, J.; Beard, S.; Dicken, D.; García-Marín, M.; Geers, V.; Glasse, A.; Glauser, A.; Gordon, K.; Justtanont, K.; Klaassen, P.; Lahuis, F.; Law, D.; Morrison, J.; Müller, M.; Rieke, G.; Vandenbussche, B.; Wright, G.

    2016-01-01

    The Mid-Infrared Instrument (MIRI) Medium Resolution Spectrometer (MRS) is the only mid-IR Integral Field Spectrometer on board James Webb Space Telescope. The complexity of the MRS requires a very specialized pipeline, with some specific steps not present in other pipelines of JWST instruments,

  7. Separation medium containing thermally exfoliated graphite oxide

    Science.gov (United States)

    Prud'homme, Robert K. (Inventor); Aksay, Ilhan A. (Inventor); Herrera-Alonso, Margarita (Inventor)

    2012-01-01

    A separation medium, such as a chromatography filling or packing, containing a modified graphite oxide material, which is a thermally exfoliated graphite oxide with a surface area of from about 300 m.sup.2/g to 2600 m.sup.2/g, wherein the thermally exfoliated graphite oxide has a surface that has been at least partially functionalized.

  8. Radiculography with water-soluble contraste medium

    International Nuclear Information System (INIS)

    Araujo Pinheiro, R.S. de

    1987-01-01

    The etiologic diagnosis of the lumbar pain is discussed. The radiculography with water-soluble contrast medium is used and 250 cases are studied. Some practical criteria of indication executation and interpretation of the examination are reported. (M.A.C.) [pt

  9. The Digital Medium Meets the Advertising Message.

    Science.gov (United States)

    Nisenholtz, Martin

    1994-01-01

    Discusses the likelihood that companies will use online services as an advertising medium. Topics addressed include the art of interactive marketing; advertising in the digital age; early experiments with interactive marketing, including the use of videotex and videodisc; and recent trends that set the stage for interactive marketing to personal…

  10. Influence of small and medium building engineering ...

    African Journals Online (AJOL)

    This study investigated the influence of small and medium building engineering entrepreneurship for the actualization of vision 20:2020 in Kebbi State, Nigeria. The study was conducted in Birnin Kebbi, Zuru and Argungu Local Government Areas of Kebbi State. The data were obtained from both primary and secondary ...

  11. Sound Art. Klang als Medium der Kunst

    DEFF Research Database (Denmark)

    Forskningsformidling på udstilling om lydkunst på ZKM i Karlsruhe. Lavet i samarbejde med Mats Lindström, leder af elektronmusikstudiet EMS (Stockholm). Udstillingen er en del af Morten Søndergaards (AAU) præsentation af skandinavisk lydkunst under titlen 'Unheard Avantgarde', der igen er en del ...... den store udstilling 'Sound as a Medium of Art'....

  12. Medium corrections to nucleon-nucleon interactions

    International Nuclear Information System (INIS)

    Dortmans, P.J.; Amos, K.

    1990-01-01

    The Bethe-Goldstone equations have been solved for both negative and positive energies to specify two nucleon G-matrices fully off of the energy shell. Medium correction effects of Pauli blocking and of the auxiliary potential are included in infinite matter systems characterized by fermi momenta in the range 0.5 fm -1 to 1.8 fm -1 . The Paris interaction is used as the starting potential in most calculations. Medium corrections are shown to be very significant over a large range of energies and densities. On the energy shell values of G-matrices vary markedly from those of free two nucleon (NN) t-matrices which have been solved by way of the Lippmann-Schwinger equation. Off of the energy shell, however, the free and medium corrected Kowalski-Noyes f-ratios rate are quite similar suggesting that a useful model of medium corrected G-matrices are appropriately scaled free NN t-matrices. The choice of auxiliary potential form is also shown to play a decisive role in the negative energy regime, especially when the saturation of nuclear matter is considered. 30 refs., 7 tabs., 7 figs

  13. Fluidized bed dry dense medium coal beneficiation

    CSIR Research Space (South Africa)

    North, Brian C

    2017-10-01

    Full Text Available medium beneficiation using a fluidized bed was investigated. Bed materials of sand, magnetite and ilmenite were used in a laboratory sized cylindrical fluidized bed. The materials were individually tested, as were mixes of sand and heavy minerals. Coal...

  14. Phase object retrieval through scattering medium

    Science.gov (United States)

    Zhao, Ming; Zhao, Meijing; Wu, Houde; Xu, Wenhai

    2018-05-01

    Optical imaging through a scattering medium has been an interesting and important research topic, especially in the field of biomedical imaging. However, it is still a challenging task due to strong scattering. This paper proposes to recover the phase object behind the scattering medium from one single-shot speckle intensity image using calibrated transmission matrices (TMs). We construct the forward model as a non-linear mapping, since the intensity image loses the phase information, and then a generalized phase retrieval algorithm is employed to recover the hidden object. Moreover, we show that a phase object can be reconstructed with a small portion of the speckle image captured by the camera. The simulation is performed to demonstrate our scheme and test its performance. Finally, a real experiment is set up, we measure the TMs from the scattering medium, and then use it to reconstruct the hidden object. We show that a phase object of size 32 × 32 is retrieved from 150 × 150 speckle grains, which is only 1/50 of the speckles area. We believe our proposed method can benefit the community of imaging through the scattering medium.

  15. Intergalactic medium heating by dark matter

    NARCIS (Netherlands)

    Ripamonti, E.; Mapelli, M.; Ferrara, A.

    2006-01-01

    Abstract: We derive the evolution of the energy deposition in the intergalactic medium (IGM) by dark matter (DM) decays/annihilations for both sterile neutrinos and light dark matter (LDM) particles. At z > 200 sterile neutrinos transfer a fraction f_abs~0.5 of their rest mass energy into the IGM;

  16. Nuclear matter as a nonlinear optical medium

    International Nuclear Information System (INIS)

    Hefter, E.F.; Papini, G.

    1986-01-01

    This paper is concerned with the question whether nuclear matter should be considered as a nonlinear optical medium. Taking, in a pragmatic way, quality and quantity of the results of well-established linear and nonlinear approaches as the main criterion, an affirmative answer is seen to be consistent with long-standing practices adhered to in nuclear physics

  17. Small and Medium Enterprises and Biopharmaceutical Innovations ...

    African Journals Online (AJOL)

    Small and Medium Enterprises and Biopharmaceutical Innovations in Africa: ... through biotechnological processes with links to biological sources especially those of live ... There are more than 500 biopharmaceutical products that have been ... By Country · List All Titles · Free To Read Titles This Journal is Open Access.

  18. Medium optimization for endochitinase production by recombinant ...

    African Journals Online (AJOL)

    The optimal concentrations of three factors were investigated by the response surface methodology using Box- Behnken design. The optimal medium components obtained for achieving the maximum activity of the endochitinase were as follows: Yeast extract 24.36 g/l, tryptone 20 g/l, YNB 5.0 g/l, potassium phosphate 100 ...

  19. Oyster mycelium on the liquid medium

    Directory of Open Access Journals (Sweden)

    Mariusz Gapiński

    2013-12-01

    Full Text Available The research presents the results of oyster mycelium growth on the liquid medium. The growth of 4-mycelium genius: Pleurotus citrinopileatus Singer, Pleurotus djamor Fries, Boedjin, Pleurotus erynii Fr. Kumm. and Pleurotus precoce Fr. Quel was tested. The quality and quantity of mycelium was assumed.

  20. Borromean structures in medium-heavy nuclei

    DEFF Research Database (Denmark)

    Hove, Dennis; Fedorov, Dmitri Vladimir; Fynbo, Hans Otto Uldall

    2014-01-01

    Borromean nuclear cluster structures are expected at the corresponding driplines. We locate the regions in the nuclear chart with the most promising constituents, it being protons and alpha-particles and investigate in details the properties of the possible borromean two-alpha systems in medium h...... 134Te−α−α structure in its ground state and low-lying spectrum....

  1. Medium Modifications of Hadrons in Photon Induced Reactions

    International Nuclear Information System (INIS)

    Schadmand, S.

    2004-01-01

    Indications for in-medium modifications of hadron properties are reported from photoabsorption and meson production experiments. Strong medium modifications are observed in inclusive photoabsorption experiments and theoretical models investigate the in-medium dynamics of baryon resonances and their coupling to mesons. Recent experiments study the in-medium behavior of scalar and vector mesons where theoretical models expect in-medium modifications of the meson spectral functions that might be connected to partial restoration of chiral symmetry

  2. Medium dependence of multiplicity distributions in MLLA

    Energy Technology Data Exchange (ETDEWEB)

    Armesto, Nestor; Pajares, Carlos; Quiroga-Arias, Paloma [Universidade de Santiago de Compostela, Departamento de Fisica de Particulas and IGFAE, Santiago de Compostela (Spain)

    2009-06-15

    We study the modification of the multiplicity distributions in MLLA due to the presence of a QCD medium. The medium is introduced through a multiplicative constant (f{sub med}) in the soft infrared parts of the kernels of the QCD evolution equations. Using the asymptotic ansatz for mean multiplicities of the quark and gluons, left angle n{sub G} right angle =e{sup {gamma}}{sup y} and left angle n{sub Q} right angle =r {sup -1}e{sup {gamma}}{sup y}, respectively, we study two cases: fixed {gamma} as previously considered in the literature, and fixed {alpha}{sub s}. We find opposite behaviors of the dispersion of the multiplicity distributions with increasing f{sub med} in both cases. For fixed {gamma} the dispersion decreases, while for fixed {alpha}{sub s} it increases. (orig.)

  3. Medium dependence of multiplicity distributions in MLLA

    International Nuclear Information System (INIS)

    Armesto, Nestor; Pajares, Carlos; Quiroga-Arias, Paloma

    2009-01-01

    We study the modification of the multiplicity distributions in MLLA due to the presence of a QCD medium. The medium is introduced through a multiplicative constant (f med ) in the soft infrared parts of the kernels of the QCD evolution equations. Using the asymptotic ansatz for mean multiplicities of the quark and gluons, left angle n G right angle =e γy and left angle n Q right angle =r -1 e γy , respectively, we study two cases: fixed γ as previously considered in the literature, and fixed α s . We find opposite behaviors of the dispersion of the multiplicity distributions with increasing f med in both cases. For fixed γ the dispersion decreases, while for fixed α s it increases. (orig.)

  4. Teaching in English-medium programmes

    DEFF Research Database (Denmark)

    Lauridsen, Karen M.; Cozart, Stacey Marie

    in such a way that they take into account their students’ diverse cultural and linguistic backgrounds and use them as a strength in the classroom; and they should be able to engage all students in joint learning activities so that both the Danish and the international students benefit from the programme......This contribution describes and discusses the module Teaching in English-medium programmes, an elective module offered as part of the teacher training programme for assistant professors (“adjunktpædagogikum”) at Aarhus University. In order to complete the whole programme, assistant professors must...... have at least one such elective module (http://upnet.au.dk/adjunktkursus/). Aarhus University offers the teacher training programme in Danish and in English for international faculty. Teaching in English-medium programmes is part of the Danish track, but taught through English. Building...

  5. Physics of the interstellar and intergalactic medium

    CERN Document Server

    Draine, Bruce T

    2010-01-01

    This is a comprehensive and richly illustrated textbook on the astrophysics of the interstellar and intergalactic medium--the gas and dust, as well as the electromagnetic radiation, cosmic rays, and magnetic and gravitational fields, present between the stars in a galaxy and also between galaxies themselves. Topics include radiative processes across the electromagnetic spectrum; radiative transfer; ionization; heating and cooling; astrochemistry; interstellar dust; fluid dynamics, including ionization fronts and shock waves; cosmic rays; distribution and evolution of the interstellar medium; and star formation. While it is assumed that the reader has a background in undergraduate-level physics, including some prior exposure to atomic and molecular physics, statistical mechanics, and electromagnetism, the first six chapters of the book include a review of the basic physics that is used in later chapters. This graduate-level textbook includes references for further reading, and serves as an invaluable resourc...

  6. Ciliated cells in vitamin A-deprived cultured hamster tracheal epithelium do divide

    International Nuclear Information System (INIS)

    Rutten, A.A.; Beems, R.B.; Wilmer, J.W.; Feron, V.J.

    1988-01-01

    The pseudostratified tracheal epithelium, composed of a heterogeneous phenotypically varying cell population, was studied with respect to the in vitro cell proliferative activity of differentiated epithelial cells. Ciliated tracheal epithelial cells so far have been considered to be terminally differentiated, nonproliferating cells. Tracheal organ cultures obtained from vitamin A-deprived Syrian Golden hamsters were cultured in a vitamin A-deficient, serum-free, hormone-supplemented medium. In vitamin A-deprived tracheal epithelium treated with physiologically active all-trans retinol and low cigarette-smoke condensate concentrations it is possible to stimulate the cell proliferation of both basal and columnar cells. Therefore, the probability of finding proliferating columnar cells was increased compared with the in vivo and the vitamin A-deprived situation in which cell proliferative activity is relatively low. In the presence of cigarette-smoke condensate in a noncytotoxic concentration, basal, small mucous granule, ciliated, and indifferent tracheal epithelial cells incorporated [methyl-3H]-thymidine into the DNA during the S phase. The finding that ciliated cells were labeled was supported by serial sections showing the same labeled ciliated cell in two section planes separated by 2 to 3 micron, without labeled epithelial cells next to the ciliated cell. Furthermore, a ciliated tracheal epithelial cell incorporating [methyl- 3 H]thymidine into DNA was also seen in tracheal cultures of vitamin A-deprived hamsters treated with all-trans retinol in a physiologic concentration

  7. El Naschie's coherence on the subquantum medium

    International Nuclear Information System (INIS)

    Agop, M.; Ioannou, P.D.; Nica, P.; Galusca, G.; Stefan, M.

    2005-01-01

    In the hydrodynamic formulation of the Scale Relativity theory one shows that a stable vortices distribution of bipolaron type induces superconducting pairs by means of the quantum potential. One builds the superconducting fractal by an iterated map and demonstrates that the superconducting pairs results as projections of this fractal. Thus, usual mechanisms (as example the exchange interaction used in the bipolaron theory) are reduced to the coherence on the subquantum medium in a ε (∞) space (El Naschie's coherence)

  8. Business Model Innovation for Small Medium Enterprises

    OpenAIRE

    Wirania Swasty

    2015-01-01

    Indonesian economy through Small Medium Enterprises (SMEs) is expected to absorb labor and contribute to the growth of Gross Domestic Product. However, SMEs lack both managerial and technical skills. This research is about business model innovation for SMEs especially in fashion and garment industry. Study used qualitative approach by mentoring four selected SMEs in Babakan Penghulu Village– Cinambo Sub-District, Eastern Bandung. The tools used to analyze them including PEST analysis, Porter’...

  9. Medium-energy ion reflection from solids

    CERN Document Server

    Mashkova, ES

    1985-01-01

    ``Medium-Energy Ion Reflection from Solids'' analyses the results of experimental, theoretical and computer investigations on the process of scattering of ions by solid surfaces. Surface scattering is a relatively young and rapidly developing branch of the physics of atomic collisions and the literature on this subject has rapidly grown.As the first monograph devoted specifically to surface scattering of ions, this book is directed at scientists involved in ion-solid interaction studies.

  10. On accretion from an inhomogeneous medium

    International Nuclear Information System (INIS)

    Davies, R.E.; Pringle, J.E.

    1980-01-01

    Hypersonic accretion flow in two dimensions from an infinite medium which contains a small density and/or velocity gradient is considered. To first order in rsub(a)/h, where rsub(a) is the accretion radius and h the scale of the gradient, the accretion rate is unaffected and the accreted angular momentum is zero. Thus previous estimates of the amount of angular momentum accreted may severely overestimate the actual value. (author)

  11. Small and medium power reactors 1985

    International Nuclear Information System (INIS)

    1986-05-01

    This report is intended for designers and planners concerned with Small and Medium Power Reactors. It provides a record of the presentations during the meetings held on this subject at the Agency's General Conference in September 1985. This information should be useful as it indicates the principal findings and main conclusions and recommendations resulting from these meetings. A separate abstract was prepared for each of the 10 presentations in this report

  12. Radiculography with reduced amounts of contrast medium

    International Nuclear Information System (INIS)

    Signorini, E.; Caputo, N.; Chiurulla, C.; Ciorba, E.; Pelliccioli, G.P.; Cianciulli, E.

    1984-01-01

    Because of the frequency and seriousness of side effects observed after radiculography, due to, among the other things, the amount of contrast medium, the authors describe a method that gives diagnostically satisfactory results using a low dose of contrast and a tangent-beam technique. Among 106 patients undergoing radiculography with a tangent-beam technique and Iopamidol, only 4 developed mild side effects, namely headache in three cases and headache with nausea in one. (Author)

  13. Leeuwpan fine coal dense medium plant

    CSIR Research Space (South Africa)

    Lundt, M

    2010-11-01

    Full Text Available Beneficiation 2010, 4–6 May 2010. 671The Journal of The Southern African Institute of Mining and Metallurgy VOLUME 110 NOVEMBER 2010 L Leeuwpan fine coal dense medium plant mixed with magnetite in the launder and enters... with production. Plant equipment operational changes Cyclone spigot changes In an attempt to lower the cut-point density, the spigot on the L 672 NOVEMBER 2010 VOLUME 110 The Journal of The Southern African Institute of Mining and Metallurgy Figure 1...

  14. Wave modulation in a nonlinear dispersive medium

    International Nuclear Information System (INIS)

    Kim, Y.C.; Khadra, L.; Powers, E.J.

    1980-01-01

    A model describing the simultaneous amplitude and phase modulation of a carrier wave propagating in a nonlinear dispersive medium is developed in terms of nonlinear wave-wave interactions between the sidebands and a low frequency wave. It is also shown that the asymmetric distribution of sidebands is determined by the wavenumber dependence of the coupling coefficient. Digital complex demodulation techniques are used to study modulated waves in a weakly ionized plasma and the experimental results support the analytical model

  15. Medium energy probes and nuclear structure

    International Nuclear Information System (INIS)

    Ginocchio, J.N.

    1984-01-01

    In this paper we explore two topics. The first topic is the marriage of medium energy reaction theory with the interacting boson model of nuclei in such a way that the multiple scattering is summed to all orders. The second topic is an exactly solvable potential model which gives realistic shell model eigenfunctions which can be used to calculate static and transition nuclear densities. (orig./HSI)

  16. Dynamical Model about Rumor Spreading with Medium

    Directory of Open Access Journals (Sweden)

    Xiaxia Zhao

    2013-01-01

    Full Text Available Rumor is a kind of social remark, that is untrue, and not be confirmed, and spreads on a large scale in a short time. Usually, it can induce a cloud of pressure, anxiety, and panic. Traditionally, it is propagated by word of mouth. Nowadays, with the emergence of the internet, rumors can be spread by instant messengers, emails, or publishing. With this new pattern of spreading, an ISRW dynamical model considering the medium as a subclass is established. Beside the dynamical analysis of the model, we mainly explore the mechanism of spreading of individuals-to-individuals and medium-to-individual. By numerical simulation, we find that if we want to control the rumor spreading, it will not only need to control the rate of change of the spreader subclass, but also need to control the change of the information about rumor in medium which has larger influence. Moreover, to control the effusion of rumor is more important than deleting existing information about rumor. On the one hand, government should enhance the management of internet. On the other hand, relevant legal institutions for punishing the rumor creator and spreader on internet who can be tracked should be established. Using this way, involved authorities can propose efficient measures to control the rumor spreading to keep the stabilization of society and development of economy.

  17. Medium temperature carbon dioxide gas turbine reactor

    International Nuclear Information System (INIS)

    Kato, Yasuyoshi; Nitawaki, Takeshi; Muto, Yasushi

    2004-01-01

    A carbon dioxide (CO 2 ) gas turbine reactor with a partial pre-cooling cycle attains comparable cycle efficiencies of 45.8% at medium temperature of 650 deg. C and pressure of 7 MPa with a typical helium (He) gas turbine reactor of GT-MHR (47.7%) at high temperature of 850 deg. C. This higher efficiency is ascribed to: reduced compression work around the critical point of CO 2 ; and consideration of variation in CO 2 specific heat at constant pressure, C p , with pressure and temperature into cycle configuration. Lowering temperature to 650 deg. C provides flexibility in choosing materials and eases maintenance through the lower diffusion leak rate of fission products from coated particle fuel by about two orders of magnitude. At medium temperature of 650 deg. C, less expensive corrosion resistant materials such as type 316 stainless steel are applicable and their performance in CO 2 have been proven during extensive operation in AGRs. In the previous study, the CO 2 cycle gas turbomachinery weight was estimated to be about one-fifth compared with He cycles. The proposed medium temperature CO 2 gas turbine reactor is expected to be an alternative solution to current high-temperature He gas turbine reactors

  18. Does culture medium influence offspring birth weight?

    Science.gov (United States)

    Carrasco, Beatriz; Boada, Montserrat; Rodríguez, Ignacio; Coroleu, Buenaventura; Barri, Pedro N; Veiga, Anna

    2013-11-01

    To determine whether the type of medium used to culture human embryos in vitro influences neonatal birth weight after IVF/intracytoplasmic sperm injection (ICSI). A prospective study and a retrospective study. Private assisted reproduction center. The prospective study included 449 IVF/ICSI cycles from August to December 2008. The retrospective analysis was performed for 2,518 IVF/ICSI cycles from October 2006 to December 2010. In the prospective study, patients were randomized for embryo culture in Cook or Vitrolife medium. The retrospective study was performed with three different culture media (MediCult, Cook, and Vitrolife). Mean birth weight, adjusted for gestational age and gender (z score) of newborns. In the prospective study, the average z score was -0.19 ± 0.85 in Cook and 0.08 ± 1.40 in Vitrolife. In the retrospective study, the z scores obtained in each group were as follows: Cook, -0.14 ± 0.96; MediCult, 0.06 ± 1.13; and Vitrolife, 0.03 ± 1.05. No significant differences were observed regarding the birth weight of children born in the different groups in both studies. The results do not show any relationship between the medium used for in vitro culture and mean birth weight adjusted for gestational age and gender of singletons born after IVF/ICSI. Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  19. Medium-Term Oil Market Report 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    How much oil will the world consume in 2011? What role will OPEC play in global oil production? Will biofuels become an important part of the oil market? The International Energy Agencys (IEA) Medium-Term Oil Market Report tackles these questions, adopting a perspective that goes beyond the traditional short-term market analysis provided in the IEAs monthly Oil Market Report. Drawing on current futures curves and the investment threshold for upstream projects, the Medium-Term Oil Market Report analyses how global demand and supply balances may develop in the next five years. The forecasts look in detail at product demand and the supply potential from all the firmly planned individual upstream and downstream projects around the world. The results provide invaluable insights on vital issues such as surplus production capacity and product supply. The rapid pace of change in the oil market means that forecasts can become outdated very quickly. This interim update provides the opportunity to rebase the data and forecasts in the annual Medium-Term Oil Market Report and to discuss and analyse new issues affecting the oil industry. Policymakers, market analysts, energy experts and anyone interested in understanding and following trends in the oil market should find this report extremely useful.

  20. Medium-Term Oil Market Report 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    How much oil will the world consume in 2012? What role will OPEC play in global oil production? Will biofuels become an important part of the oil market? How will the refinery sector cope? The International Energy Agency (IEA) Medium-Term Oil Market Report tackles these questions, adopting a perspective that goes beyond the traditional short-term market analysis provided in the IEA Oil Market Report. Drawing on current futures curves and the investment threshold for upstream projects, the Medium-Term Oil Market Report analyses how global demand and supply balances may develop. By assessing all firmly planned upstream and downstream projects worldwide, this report forecasts supply and demand potential for crude and petroleum products over the next five years. The results provide an invaluable insight into vital issues such as surplus production capacity and product supply. An essential report for all policymakers, market analysts, energy experts and anyone interested in understanding and following oil market trends, the Medium-Term Oil Market Report is a further element of the strong commitment of the IEA to improving and expanding the quality, timeliness and accuracy of energy data and analysis.

  1. Tracer transfer in consolidated porous medium and fractured porous medium: experimentations and modelling

    International Nuclear Information System (INIS)

    Dalla Costa, C.

    2007-07-01

    We try to identify and model physical and chemical mechanisms governing the water flow and the solute transport in fractured consolidated porous medium. An original experimental device was built. The 'cube' consists of an idealized fractured medium reproduced by piling up consolidated porous cubes of 5 cm edge. Meanwhile, columns of the homogeneous consolidated porous medium are studied. The same anionic tracing technique is used in both cases. Using a system analysis approach, we inject concentration pulses in the device to obtain breakthrough curves. After identifying the mass balance and the residence time, we fit the CD and the MIM models to the experimental data. The MIM model is able to reproduce experimental curves of the homogeneous consolidated porous medium better than the CD model. The mobile water fraction is in accordance with the porous medium geometry. The study of the flow rate influence highlights an interference dispersion regime. It was not possible to highlight the observation length influence in this case. On the contrary, we highlight the effect of the observation scale on the fractured and porous medium, comparing the results obtained on a small 'cube' and a big 'cube'. The CD model is not satisfactory in this case. Even if the MIM model can fit the experimental breakthrough curves, it was not possible to obtain unique parameters for the set of experiments. (author)

  2. Acceleration of diabetic wound healing with adipose-derived stem cells, endothelial-differentiated stem cells, and topical conditioned medium therapy in a swine model.

    Science.gov (United States)

    Irons, Robin F; Cahill, Kevin W; Rattigan, Deviney A; Marcotte, Joseph H; Fromer, Marc W; Chang, Shaohua; Zhang, Ping; Behling, Eric M; Behling, Kathryn C; Caputo, Francis J

    2018-05-09

    The purpose of our study was to investigate the effect of adipose-derived stem cells (ASCs), endothelial-differentiated ASCs (EC/ASCs), and various conditioned media (CM) on wound healing in a diabetic swine model. We hypothesized that ASC-based therapies would accelerate wound healing. Diabetes was induced in four Yorkshire swine through intravenous injection of streptozotocin. ASCs were harvested from flank fat and cultured in either M199 or EGM-2 medium. A duplicate series of seven full-thickness dorsal wounds were surgically created on each swine. The wounds in the cellular treatment group underwent injection of low-dose or high-dose ASCs or EC/ASCs on day 0, with a repeat injection of one half of the initial dose on day 15. Wounds assigned to the topical CM therapy were covered with 2 mL of either serum-free M199 primed by ASCs or human umbilical vein endothelial cells every 3 days. Wounds were assessed at day 0, 10, 15, 20, and 28. The swine were sacrificed on day 28. ImageJ software was used to evaluate the percentage of wound healing. The wounded skin underwent histologic, reverse transcription polymerase chain reaction, and enzyme-linked immunosorbent assay examinations to evaluate markers of angiogenesis and inflammation. We found an increase in the percentage of wound closure rates in cell-based treatments and topical therapies at various points compared with the untreated control wounds (P swine model. Enhanced angiogenesis and immunomodulation might be key contributors to this process. The purpose of the present study was to translate the known beneficial effects of adipose-derived stem cells and associated conditioned medium therapy on diabetic wound healing to a large animal model. We demonstrated that stem cell and conditioned medium therapy significantly accelerate gross wound healing in diabetic swine, with data suggesting this might result from a decreased inflammatory response and increased angiogenesis. Copyright © 2018 Society for

  3. A medium energy neutron deep penetration experiment

    International Nuclear Information System (INIS)

    Amian, W.; Cloth, P.; Druecke, V.; Filges, D.; Paul, N.; Schaal, H.

    1986-11-01

    A deep penetration experiment conducted at the Los Alamos WNR facility's Spallation Neutron Target is compared with calculations using intra-nuclear-cascade and S N -transport codes installed at KFA-IRE. In the experiment medium energy reactions induced by neutrons between 15 MeV and about 150 MeV inside a quasi infinite slab of iron have been measured using copper foil monitors. Details of the experimental procedure and the theoretical methods are described. A comparison of absolute reaction rates for both experimentally and theoretically derived reactions is given. The present knowledge of the corresponding monitor reaction cross sections is discussed. (orig.)

  4. In medium modification of nucleon electromagnetic properties

    International Nuclear Information System (INIS)

    Khanna, F.; Rakhimov, A.; Yakhsiev, U.

    1997-01-01

    Since nucleons are composite objects, their internal structure is expected to be changed by nuclear environment. A Skyrme like Lagrangian is proposed to consider such effects, namely the modification of electromagnetic (EM) properties of the nucleon. The static properties and EM form factors were obtained. It was shown that the charge radius of the nucleon increased in medium and the mass and axial coupling constant are reduced. The enhancement of magnetic moment of proton is smaller than that obtained in non-topological soliton model.Obtained results may be useful in electron nucleus scattering analysis.(A.A.D.)

  5. The Casimir effect: medium and geometry

    International Nuclear Information System (INIS)

    Marachevsky, Valery N

    2012-01-01

    Theory of the Casimir effect is presented in several examples. Casimir–Polder-type formulas, Lifshitz theory and theory of the Casimir effect for two gratings separated by a vacuum slit are derived. Equations for the electromagnetic field in the presence of a medium and dispersion are discussed. The Casimir effect for systems with a layer of 2 + 1 fermions is studied. This article is part of a special issue of Journal of Physics A: Mathematical and Theoretical in honour of Stuart Dowker's 75th birthday devoted to ‘Applications of zeta functions and other spectral functions in mathematics and physics’. (paper)

  6. Relativistic energy loss in a dispersive medium

    DEFF Research Database (Denmark)

    Houlrik, Jens Madsen

    2002-01-01

    The electron energy loss in a dispersive medium is obtained using macroscopic electrodynamics taking advantage of a static frame of reference. Relativistic corrections are described in terms of a dispersive Lorentz factor obtained by replacing the vacuum velocity c by the characteristic phase...... velocity c/n, where n is the complex index of refraction. The angle-resolved energy-loss spectrum of a Drude conductor is analyzed in detail and it is shown that the low-energy peak due to Ohmic losses is enhanced compared to the classical approximation....

  7. Interactions between intergalactic medium and galaxies

    International Nuclear Information System (INIS)

    Einasto, J.; Saar, E.

    1977-01-01

    The interaction of galaxies with the environmental gas both in clusters and in small groups of galaxies is investigated. Interaction between galaxies and the ambient medium can be considered simply as final touches in the process of galaxy formation. Large relative velocities of galaxies in their clusters and of the intercluster gas result in a loss of the intergalactic gas, that in its turn affects the morphology of cluster galaxies. Interaction between the coronal clouds and the gas in the disk of spiral galaxies may result in regular patterns of star formation and in the bending of planes of galaxies

  8. Adiabatic supernova expansion into the circumstellar medium

    International Nuclear Information System (INIS)

    Band, D.L.; Liang, E.P.

    1987-01-01

    We perform one dimensional numerical simulations with a Lagrangian hydrodynamics code of the adiabatic expansion of a supernova into the surrounding medium. The early expansion follows Chevalier's analytic self-similar solution until the reverse shock reaches the ejecta core. We follow the expansion as it evolves towards the adiabatic blast wave phase. Some memory of the earlier phases of expansion is retained in the interior even when the outer regions expand as a blast wave. We find the results are sensitive to the initial configuration of the ejecta and to the placement of gridpoints. 6 refs., 2 figs

  9. Medium energy hadron scattering from nuclei

    International Nuclear Information System (INIS)

    Ginocchio, J.N.; Wenes, G.

    1986-01-01

    The Glauber approximation for medium energy scattering of hadronic projectiles from nuclei is combined with the interacting boson model of nuclei to produce a transition matrix for elastic and inelastic scattering in algebraic form which includes coupling to all the intermediate states. We present closed form analytic expresions for the transition matrix elements for the three dynamical symmetries of the interacting boson model; that is for, a spherical quadrupole vibrator, a γ unstable rotor, and both prolate and oblate axially symmetric rotors. We give examples of application of this formalism to proton scattering from 154 Sm and 154 Gd. 27 refs., 5 figs., 1 tab

  10. Polycyclic aromatic hydrocarbons in stellar medium

    Science.gov (United States)

    Rastogi, Shantanu

    2005-06-01

    Polycyclic Aromatic Hydrocarbons (PAHs) are an important com- ponent of the Interstellar Medium (ISM). They are being used as probes for understanding of process and conditions of different astrophysical environments. The understanding of their IR spectra and its variations with PAH size and ionization state is useful in characterizing the ISM. Spectral features of model graphene sheets and also that of smaller PAH molecules are reported. The variation of intensity with charge state of the molecule shows that cations give a better correlation with observations. The relationship between changes in charge distribution with intensity changes upon ionization has been probed.

  11. Electromagnetically Induced Transparency In Rydberg Atomic Medium

    Science.gov (United States)

    Deng, Li; Cong, Lu; Chen, Ai-Xi

    2018-03-01

    Due to possessing big principal quantum number, Rydberg atom has some unique properties, for example: its radiative lifetime is long, dipole moment is large, and interaction between atoms is strong and so on. These properties make one pay attention to Rydberg atoms. In this paper we investigate the effects of Rydberg dipole-dipole interactions on electromagnetically induced transparency (EIT) schemes and group velocity in three-level systems of ladder type, which provides theoretical foundation for exploring the linear and nonlinear characteristics of light in a Rydberg electromagnetically-induced-transparency medium.

  12. Medium modifications of nucleon electromagnetic form factors

    Energy Technology Data Exchange (ETDEWEB)

    Horikawa, T. [Department of Physics, School of Science, Tokai University, Hiratsuka-shi, Kanagawa 259-1292 (Japan); Bentz, W. [Department of Physics, School of Science, Tokai University, Hiratsuka-shi, Kanagawa 259-1292 (Japan)]. E-mail: bentz@keyaki.cc.u-tokai.ac.jp

    2005-11-28

    We use the Nambu-Jona-Lasinio model as an effective quark theory to investigate the medium modifications of the nucleon electromagnetic form factors. By using the equation of state of nuclear matter derived in this model, we discuss the results based on the naive quark-scalar diquark picture, the effects of finite diquark size, and the meson cloud around the constituent quarks. We apply this description to the longitudinal response function for quasielastic electron scattering. RPA correlations, based on the nucleon-nucleon interaction derived in the same model, are also taken into account in the calculation of the response function.

  13. Electromagnetic properties of neutrinos in a medium

    International Nuclear Information System (INIS)

    Nieves, J.F.; Pal, P.B.

    1989-01-01

    We show that, contrary to the situation in the vacuum, a Majorana neutrino can have electric and magnetic dipole moments in a medium. This is because of new contributions, equal for a particle and its antiparticle, that can arise only in a material background. For Dirac neutrinos, these contributions make the magnitudes of the dipole moments of the particle and the antiparticle unequal. We discuss the conditions which give rise to such effects, with particular attention to the role played by the discrete symmetries C, P, and T

  14. Nuclear medium effects on the nucleon properties

    International Nuclear Information System (INIS)

    Gerard, A.

    1986-06-01

    The idea that the nuclear medium may modify the properties of the nucleon is not really a new one. But until these last few years the intrinsic properties of the nucleon such as its mass, baryonic or electromagnetic size, magnetic moments, were hardly suspected to be substantially modified by the nuclear environment. The question raised essentially from two categories of experimental investigations, in quite different kinematical regimes and it remains at the present time a controversal subject. In this paper I try to review the present status of experimental investigations and some of the recent theoretical developments relevant to this topic

  15. Cooperative networking in a heterogeneous wireless medium

    CERN Document Server

    Ismail, Muhammad

    2013-01-01

    This brief focuses on radio resource allocation in a heterogeneous wireless medium. It presents radio resource allocation algorithms with decentralized implementation, which support both single-network and multi-homing services. The brief provides a set of cooperative networking algorithms, which rely on the concepts of short-term call traffic load prediction, network cooperation, convex optimization, and decomposition theory. In the proposed solutions, mobile terminals play an active role in the resource allocation operation, instead of their traditional role as passive service recipients in the networking environment.

  16. Distributed medium access control in wireless networks

    CERN Document Server

    Wang, Ping

    2013-01-01

    This brief investigates distributed medium access control (MAC) with QoS provisioning for both single- and multi-hop wireless networks including wireless local area networks (WLANs), wireless ad hoc networks, and wireless mesh networks. For WLANs, an efficient MAC scheme and a call admission control algorithm are presented to provide guaranteed QoS for voice traffic and, at the same time, increase the voice capacity significantly compared with the current WLAN standard. In addition, a novel token-based scheduling scheme is proposed to provide great flexibility and facility to the network servi

  17. Superluminous laser pulse in an active medium

    International Nuclear Information System (INIS)

    Fisher, D.L.; Tajima, T.

    1993-12-01

    Physical conditions are obtained to make the propagation velocity of a laser pulse and thus the phase velocity of the excited wake be at any desired value, including that equal to or greater than the speed of light. The provision of an active-plasma laser medium with an appropriately shaped pulse allows not only replenishment of laser energy loss to the wakefield but also acceleration of the group velocity of photons. A stationary solitary solution in the accelerated frame is obtained from the model equations and simulations thereof for the laser, plasma and atoms. This approach has applications in photonics and telecommunications as well as wakefield accelerators

  18. The interstellar medium in galaxies - An overview

    Science.gov (United States)

    Knapp, G. R.

    1990-01-01

    Recent observational developments on the subject of the interstellar medium in galaxies are summarized, with emphasis placed on global properties. The properties and distribution of the ISM in the solar neighborhood and in the Galactic plane are examined and a number of results from the most important observational probes (HI, CO, and infrared) are described. A recent development is the observation of the ISM in galaxies of all morphological types, early to late. These developments are summarized and the properties of different types of galaxies are compared to one another. The origin of radio galaxies, the effect of environment, and the prospects for direct observations of ISM evolution in galaxies are discussed.

  19. Managing Safety in Small and Medium Enterprises

    DEFF Research Database (Denmark)

    Stephen, legg; Olsen, Kirsten Bendix; Ian S., laird

    2015-01-01

    on safety in SMEs, showing how most current policy and legislation on occupational health and safety (OSH) and the work environment is based on large enterprises and that there is a relative paucity of research on OSH in SMEs. In a summary of current knowledge, it is argued that modern OHS legislation......This paper presents a conceptual model for increasing acceptable working environments for SMEs. It also acts as an editorial for the special issue of Safety Science on ‘Managing safety in small and medium enterprises (SMEs)’. It describes how seven of the ten papers in the special issue originate...

  20. Laser light scattering in Brownian medium

    International Nuclear Information System (INIS)

    Suwono; Santoso, Budi; Baiquni, A.

    1983-01-01

    The principle of laser light scattering in Brownian medium and photon correlation spectroscopy are described in detail. Their application to the study of the behaviour of a polystyrene latex solution are discussed. The auto-correlation function of light scattered by the polystyrene latex solution in various angle, various temperature and in various sample times, have been measured. Information on the translation diffusion coefficient and size on the particle can be obtained from the auto-correlation function. Good agreement between the available data and experiment is shown. (author)

  1. The question of caution in professional medium

    International Nuclear Information System (INIS)

    Godard, O.

    2006-01-01

    Contrived in Europe to tackle the environment protection policies and management of natural resources, the principle of caution has seen since 1990 its interest developed towards the fields of food safety and public health. The importance and the relevance of questions have lead the national institute of research and safety to constitute a working group to explore the problems and the potentialities that could be linked to the explicit introduction of this principle and this in the field of the prevention of professional risks. This work tackles several aspects of the question of caution in professional medium such history, concepts, evolution...as well as cases judged exemplary ones. (N.C.)

  2. Degradation testing of Mg alloys in Dulbecco's modified eagle medium: Influence of medium sterilization.

    Science.gov (United States)

    Marco, Iñigo; Feyerabend, Frank; Willumeit-Römer, Regine; Van der Biest, Omer

    2016-05-01

    This work studies the in vitro degradation of Mg alloys for bioabsorbable implant applications under near physiological conditions. For this purpose, the degradation behaviour of Mg alloys in Dulbecco's modified eagle medium (DMEM) which is a commonly used cell culture medium is analysed. Unfortunately, DMEM can be contaminated by microorganisms, acidifying the medium and accelerating the Mg degradation process by dissolution of protective degradation layers, such as (Mgx,Cay)(PO4)z. In this paper the influence of sterilization by applying UV-C radiation and antibiotics (penicillin/streptomycin) is analysed with two implant material candidates: Mg-Gd and Mg-Ag alloys; and pure magnesium as well as Mg-4Y-3RE as a reference. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Characterisation of Ferrosilicon Dense Medium Separation Material

    International Nuclear Information System (INIS)

    Waanders, F. B.; Mans, A.

    2003-01-01

    Ferrosilicon is used in the dense medium separation of iron ore at Kumba resources, Sishen, South Africa. Due to high cost and losses that occur during use, maximum recovery by means of magnetic separation is aimed for. The purpose of this project was to determine the characteristics of the unused Fe-Si and then to characterise the changes that occur during storage and use thereof. Scanning electron microscopy was used to determine the composition of each sample, whilst Moessbauer spectroscopy yielded a two-sextet spectrum with hyperfine magnetic field strengths of 20 and 31 T, respectively, for the fresh samples. Additional hematite oxide peaks appeared in the Moessbauer spectra after use of the Fe-Si over a length of time, but this did not result in a dramatic degradation of the medium. No definite changes occurred during correct storage methods. It was, however, found that the biggest loss of Fe-Si was due to the abrasion of the particles, which resulted in the formation of an oxihydroxide froth, during the process.

  4. Perspective on small and medium size reactors

    International Nuclear Information System (INIS)

    Stahlkopf, K.E.; Braun, C.

    1985-01-01

    There has been renewed interests in the United States in small and medium size nuclear power plants (600 MWe or less). The reasons for this include: (1) there is a large uncertainty in load growth projections. Small to medium size nuclear power plants may be better suited to meet uncertain load growth projections. (2) It has been argued that a large economy of scale exists because of the nature of nuclear power installations. A recent examination of the French program shows that no economy of scale exists between the 900 MWe and 1300 MWe plants. Others have suggested that it is possible to reduce the economy of scale so it is not a prohibitive factor. (3) In the past in the United States, it has been customary for several smaller utilities to share the output of a large nuclear plant to take advantage of the perceived economy of scale. Difficulties have been encountered by some of these enterprises. (4) An examination of capacity factors for the United States shows that plants of smaller output appear to operate more reliably and economically than larger plants

  5. GABA interaction with lipids in organic medium

    International Nuclear Information System (INIS)

    Beltramo, D.; Kivatinitz, S.; Lassaga, E.; Arce, A.

    1987-01-01

    The interaction of 3 H-GABA and 14 C-glutamate with lipids in an aqueous organic partition system was studied. With this partition system 3 H-GABA and 14 C-glutamate were able to interact with sphingomyelin, sulfatide, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidic acid but not with cholesterol or ceramide. In an homogeneous aqueous medium the authors could not demonstrate any interaction between 3 H-GABA-lipids. The apparent dissociation constants (K/sub d/) for 3 H-GABA-lipids or 14 C-glutamate-lipids interactions inorganic medium were in the millimolar range and maximal charge between 3 and 7 moles of GABA or glutamate by mole of lipid. Amino acids such as glutamic acid, β-alanine and glycine displaced 3 H-GABA with the same potency as GABA itself; thus these results show that the interaction lacks pharmacological specificity. To detect this interaction lipid concentrations higher than 2 μM were required and in the partition system 3 H-GABA and lipid phosphorus were both concentrated at the interface. Therefore, lipids tested with a biphasic partition system do not fulfill the classical criteria for a neurotransmitter receptor at least not for GABA and glutamate. 15 references, 1 figure, 3 tables

  6. Sector boundary distortion in the interplanetary medium

    International Nuclear Information System (INIS)

    Suess, S.T.; Feynman, J.

    1977-01-01

    We address the theoretical problem of the effect of a solar wind meridional velocity gradient on the orientation, or tipping, of a line embedded within the interplanetary plasma. We find that rotations of from 30degree to 75degree, between 1.5 solar radii and I AU, are produced when observed values for the solar wind velocity and its meridional gradient are used. This is not a small effect, nor is it difficult to calculate: it is a natural consequence of any meridional velocity gradient in the interplanetary medium. In relating this result to observed sector boundaries we note that the latitude dependence of the width of interplanetary magnetic sectors (dominant polarity or Rosenberg-Coleman effect) implies that sector boundaries at I AU are generally inclined at an angle of from 10degree to 20degree to the solar equatorial plane. Conversely, studies of photospheric magnetic fields have led to the conclusion that sector boundaries near the sun are, on the average, at large angles (approx.90degree) to the solar equatorial plane. If the dominant polarity effect were to be produced by rotation in the interplanetary medium, the sign of the solar wind meridional velocity gradient must not change at the equator, but the gradient does have to change sign for +/- boundary crossings in comparison to -/+ boundary crossings

  7. Medium-size power plants. Economic options

    International Nuclear Information System (INIS)

    Vogelweith, L.; Baujat, J.; Goutail, J.

    1978-01-01

    In this paper the authors describe a method for economic evaluation of a nuclear power plant project such as advocated by the IAEA but modified through the introduction of various parameters that may affect the evaluation, i.e. the weighted evaluation rate, the annual increase in the cost of fuel, and the discount rate. The method is applied to barge-based medium-size reactors (125 MW(e)). The authors calculate the investment cost, together with the costs of administration, operation and maintenance; use is made of current assumptions regarding the price of fuel for the case of a reference nuclear plant and an oil-fired plant of the same power and in the same programme. In this way the authors derive the discounted cost of the nuclear programme and concurrent conventional programme on the basis of the following assumptions: a weighted inflation rate varying between 0 and 6% per year; an annual increase in real fuel prices ranging from 0 to 3%; and a real discount rate, equal to the real interest rate, varying between 4 and 7% per year and corresponding to nominal discount rates of up to 13.4%. The conclusion reached is that, given the real interest rates actually prevailing on the financial market, a weighted inflation rate foreseen by the majority of experts, and a rise in real fuel prices of the order of 1% per year, the medium-size nuclear power plant is more economical than a conventional plant of the same output. (author)

  8. INNOVATIVE CULTURE IN SMALL AND MEDIUM ENTERPRISES

    Directory of Open Access Journals (Sweden)

    Aluisio Broering Mambrini

    2011-10-01

    Full Text Available In the last two decades, innovation has been a key driver of economic growth. Innovation is closely related to creating value and generating wealth through successful service to consumer needs. Thus, it is not necessarily restricted to the use of new knowledge generated from research, but on the development of new products or services that are obtained with creative use of knowledge, new or already known. This study aimed to identify management practices that promote a culture of innovation in small and medium enterprises and analyze how they contribute to the innovative capacity of these companies. The research method was the multiple case study with six small and medium businesses that have at least one case of significant innovation in its history. The main results showed that amongst the practices are: a performance in highly specialized niches and deep focus on customer needs; b strong investment and incorporation of new knowledge outside the company (open innovation; c speed and agility in the absorption and deployment of new knowledge and technologies; d retention of employees; e acting as an integrator combining diverse knowledge and technologies; f the information management of the knowledge acquired by the company; g little concern to patent the technology; h flexibility and informal, fluid and open communication between employees of the company that promotes agility in management and i the management of partnerships across the value chain, including the functional areas.

  9. Shock propagation in a heterogeneous medium

    International Nuclear Information System (INIS)

    Elbaz, D.

    2011-01-01

    In the frame of the inertial confinement fusion in direct drive, the use of foams as ablator allows the reduction of hydrodynamic instabilities created on the target by the direct laser irradiation. The foam is made up of carbon (CH) fibers impregnated of cryogenic deuterium-tritium (DT). In the past, studies have been carried out considering this foam to be a homogeneous medium. Yet, the foam presents heterogeneous features. We study the effects of this heterogeneity on the shock velocity when the laser irradiates the target. Thanks to experimental and numerical studies, we show that the shock propagates faster in the heterogeneous medium than in the homogeneous one with the same averaged density. This velocity gap depends on the presence rate of the CH fibers in the foam, the density ratio, the adiabatic coefficient and the foam geometry. We model the foam by different ways, more and more complex. The shock velocity modification is due to the baroclinicity which, during the interaction between the shock front and the interface, creates a vorticity deposition, responsible for the shock acceleration. Accordingly, an interface, which is plane and perpendicular to the front shock, maximizes the vorticity deposition and increases the velocity gaps between heterogeneous and homogeneous media. We found a correlation between the kinetic energy behind the shock front and the velocities relative difference. We compared our results with two analytical models. However, the system is not closed, so we can't for the moment develop a predictive model. (author) [fr

  10. A direct sampling method for inverse electromagnetic medium scattering

    KAUST Repository

    Ito, Kazufumi; Jin, Bangti; Zou, Jun

    2013-01-01

    In this paper, we study the inverse electromagnetic medium scattering problem of estimating the support and shape of medium scatterers from scattered electric/magnetic near-field data. We shall develop a novel direct sampling method based

  11. Casimir force in the presence of a medium

    International Nuclear Information System (INIS)

    Kheirandish, Fardin; Soltani, Morteza; Sarabadani, Jalal

    2010-01-01

    We investigate the Casimir effect in the presence of a medium by quantizing the electromagnetic field in the presence of a magnetodielectric medium using the path-integral technique. For a given medium with definite electric and magnetic susceptibilities, explicit expressions for the Casimir force are obtained. The Lifshitz formula is recovered and in the absence of a medium the results tend to the original Casimir force between two conducting parallel plates immersed in the quantum electromagnetic vacuum.

  12. Medium optimization for protopectinase production by batch culture of

    African Journals Online (AJOL)

    Medium optimization for protopectinase production by batch culture of. C Fan, Z Liu, L Yao. Abstract. Optimization of medium compositions for protopectinase production by Aspergillus terreus in submerged culture was carried out. The medium components having significant effect on protopectinase production were reported ...

  13. Transitioning from Maori-Medium to English-Medium Education: Emerging Findings of a Pilot Study

    Science.gov (United States)

    Hill, Richard

    2016-01-01

    Maori-bilingual education in New Zealand has come a long way towards injecting life into the Maori language. However, only a small number of families commit to bilingual education for the long term. This paper discusses why Maori parents either turn away from Maori-bilingual education or prematurely transition to English-medium schools. It then…

  14. Ultrasound fields in an attenuating medium

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt; Gandhi,, D; O'Brien,, W.D., Jr.

    1993-01-01

    of the rectangles and sums all contributions to arrive at the spatial impulse response for the aperture and field point. This approach makes it possible to model all transducer apertures, and the program can readily calculate the emitted, pulse-echo and continuous wave field. Attenuation is included by splitting...... it into a frequency dependent part and frequency independent part. The latter results in an attenuation factor that is multiplied onto the responses from the individual elements, and the frequency dependent part is handled by attenuating the basic one-dimensional pulse. The influence on ultrasound fields from......Ultrasound fields propagating in tissue will undergo changes in shape not only due to diffraction, but also due to the frequency dependent attenuation. Linear fields can be fairly well predicted for a non-attenuating medium like water by using the Tupholme-Stepanishen method for calculating...

  15. Study of QCD medium by sum rules

    Energy Technology Data Exchange (ETDEWEB)

    Mallik, S [Saha Institute of Nuclear Physics, Calcutta (India)

    1998-08-01

    Though it has no analogue in condensed matter physics, the thermal QCD sum rules can, nevertheless, answer questions of condensed matter type about the QCD medium. The ingredients needed to write such sum rules, viz. the operator product expansion and the spectral representation at finite temperature, are reviewed in detail. The sum rules are then actually written for the case of correlation function of two vector currents. Collecting information on the thermal average of the higher dimension operators from other sources, we evaluate these sum rules for the temperature dependent {rho}-meson parameters. Possibility of extracting more information from the combined set of all sum rules from different correlation functions is also discussed. (author) 30 refs., 2 figs.

  16. Medium Duty Electric Vehicle Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Mackie, Robin J. D. [Smith Electric Vehicles Corporation, Kansas City, MO (United States)

    2015-05-31

    The Smith Electric Vehicle Demonstration Project (SDP) was integral to the Smith business plan to establish a manufacturing base in the United States (US) and produce a portfolio of All Electric Vehicles (AEV’s) for the medium duty commercial truck market. Smith focused on the commercial depot based logistics market, as it represented the market that was most ready for the early adoption of AEV technology. The SDP enabled Smith to accelerate its introduction of vehicles and increase the size of its US supply chain to support early market adoption of AEV’s that were cost competitive, fully met the needs of a diverse set of end users and were compliant with Federal safety and emissions requirements. The SDP accelerated the development and production of various electric drive vehicle systems to substantially reduce petroleum consumption, reduce vehicular emissions of greenhouse gases (GHG), and increase US jobs.

  17. Abundances in the diffuse interstellar medium

    International Nuclear Information System (INIS)

    Harris, A.W.

    1988-04-01

    The wealth of interstellar absorption line data obtained with the Copernicus and IUE satellites has opened up a new era in studies of the interstellar gas. It is now well established that certain elements, generally those with high condensation temperatures, are substantially under-abundant in the gas-phase relative to total solar or cosmic abundances. This depletion of elements is due to the existence of solid material in the form of dust grains in the interstellar medium. Surprisingly, however, recent surveys indicate that even volatile elements such as Zn and S are significantly depleted in many sight lines. Developments in this field which have been made possible by the large base of UV interstellar absorption line data built up over recent years are reviewed and the implications of the results for our understanding of the physical processes governing depletion are discussed. (author)

  18. Polarization of submillimetre lines from interstellar medium

    Science.gov (United States)

    Zhang, Heshou; Yan, Huirong

    2018-04-01

    Magnetic fields play important roles in many astrophysical processes. However, there is no universal diagnostic for the magnetic fields in the interstellar medium (ISM) and each magnetic tracer has its limitation. Any new detection method is thus valuable. Theoretical studies have shown that submillimetre fine-structure lines are polarized due to atomic alignment by ultraviolet photon-excitation, which opens up a new avenue to probe interstellar magnetic fields. We will, for the first time, perform synthetic observations on the simulated three-dimensional ISM to demonstrate the measurability of the polarization of submillimetre atomic lines. The maximum polarization for different absorption and emission lines expected from various sources, including star-forming regions are provided. Our results demonstrate that the polarization of submillimetre atomic lines is a powerful magnetic tracer and add great value to the observational studies of the submilimetre astronomy.

  19. Cosmic rays and the interstellar medium

    International Nuclear Information System (INIS)

    Wolfendale, A.W.

    1986-01-01

    It is inevitable that there is a close connection between cosmic rays and the ISM insofar as the propagation of cosmic rays is conditioned by the magnetic field in the ISM and the cosmic rays interact with the gas (and photon fluxes) in this medium. This paper deals with both topics. Propagation effects manifest themselves as an anisotropy in arrival directions and a review is given of anisotropy measurements and their interpretation. The status of studies of cosmic ray interactions is examined whit particular reference to the information about the ISM itself which comes from observations of the flux of secondary γ-rays produced by cosmic ray interactions with gas, the situation regarding molecular as in the Inner Galaxy being of particular concern

  20. Development of novel Alicyclobacillus spp. isolation medium.

    Science.gov (United States)

    Chang, S; Kang, D-H

    2005-01-01

    To develop a new isolation medium with higher recovery rates of Alicyclobacillus spp. SK agar was developed with optimized incubation temperature, pH, acidulant, Tween 80 concentration and divalent cation addition. Results indicate that detection of Alicyclobacillus spp. by SK agar was significantly higher (P > 0.05) than those obtained by K agar, orange serum agar, and potato dextrose agar. Current media used for Alicyclobacillus spp. isolation still resulted in high numbers of false negative products. The sensitivity of SK agar to Alicyclobacillus spp. allows detection of low numbers of Alicyclobacillus spp. and also provides a more higher isolation results compared with currently used media. SK agar will be useful to the fruit juice industry to obtain more accurate numbers of contaminant Alicyclobacillus spp. With this media, false negative samples can be reduced, and the likelihood of exported products being rejected can be greatly reduced.

  1. Nonlinear dynamo in the intracluster medium

    Science.gov (United States)

    Beresnyak, Andrey; Miniati, Francesco

    2018-05-01

    Hot plasma in galaxy clusters, the intracluster medium is observed to be magnetized with magnetic fields of around a μG and the correlation scales of tens of kiloparsecs, the largest scales of the magnetic field so far observed in the Universe. Can this magnetic field be used as a test of the primordial magnetic field in the early Universe? In this paper, we argue that if the cluster field was created by the nonlinear dynamo, the process would be insensitive to the value of the initial field. Our model combines state of the art hydrodynamic simulations of galaxy cluster formation in a fully cosmological context with nonlinear dynamo theory. Initial field is not a parameter in this model, yet it predicts magnetic scale and strength compatible with observations.

  2. Light Vector Mesons in the Nuclear Medium

    Energy Technology Data Exchange (ETDEWEB)

    Wood, Michael; Nasseripour, Rakhsha; Weygand, Dennis; Djalali, Chaden; Tur, Clarisse; Mosel, Ulrich; Muehlich, Pascal; Adams, Gary; Amaryan, Moscov; Amaryan, Moskov; Ambrozewicz, Pawel; Anghinolfi, Marco; Asryan, Gegham; Avagyan, Harutyun; Baghdasaryan, Hovhannes; Baillie, Nathan; Ball, James; Baltzell, Nathan; Barrow, Steve; Battaglieri, Marco; Bedlinskiy, Ivan; Bektasoglu, Mehmet; Bellis, Matthew; Benmouna, Nawal; Berman, Barry; Biselli, Angela; Blaszczyk, Lukasz; Bouchigny, Sylvain; Boyarinov, Sergey; Bradford, Robert; Branford, Derek; Briscoe, William; Brooks, William; Burkert, Volker; Butuceanu, Cornel; Calarco, John; Careccia, Sharon; Carman, Daniel; Carnahan, Bryan; Casey, Liam; Chen, Shifeng; Cheng, Lu; Cole, Philip; Collins, Patrick; Coltharp, Philip; Crabb, Donald; Crannell, Hall; Crede, Volker; Cummings, John; Dashyan, Natalya; De Vita, Raffaella; De Sanctis, Enzo; Degtiarenko, Pavel; Denizli, Haluk; Dennis, Lawrence; Deur, Alexandre; Dharmawardane, Kahanawita; Dickson, Richard; Dodge, Gail; Doughty, David; Dugger, Michael; Dytman, Steven; Dzyubak, Oleksandr; Egiyan, Hovanes; Egiyan, Kim; Elfassi, Lamiaa; Elouadrhiri, Latifa; Eugenio, Paul; Fedotov, Gleb; Feldman, Gerald; Feuerbach, Robert; Fradi, Ahmed; Funsten, Herbert; Garcon, Michel; Gavalian, Gagik; Gilfoyle, Gerard; Giovanetti, Kevin; Girod, Francois-Xavier; Goetz, John; Gordon, Christopher; Gothe, Ralf; Griffioen, Keith; Guidal, Michel; Guler, Nevzat; Guo, Lei; Gyurjyan, Vardan; Hadjidakis, Cynthia; Hafidi, Kawtar; Hakobyan, Hayk; Hakobyan, Rafael; Hanretty, Charles; Hardie, John; Hassall, Neil; Hersman, F.; Hicks, Kenneth; Hleiqawi, Ishaq; Holtrop, Maurik; Hyde, Charles; Ilieva, Yordanka; Ireland, David; Ishkhanov, Boris; Isupov, Evgeny; Ito, Mark; Jenkins, David; Jo, Hyon-Suk; Johnstone, John; Joo, Kyungseon; Juengst, Henry; Kalantarians, Narbe; Kellie, James; Khandaker, Mahbubul; Khetarpal, Puneet; Kim, Wooyoung; Klein, Andreas; Klein, Franz; Klimenko, Alexei; Kossov, Mikhail; Krahn, Zebulun; Kramer, Laird; Kubarovsky, Valery; Kuhn, Joachim; Kuhn, Sebastian; Kuleshov, Sergey; Lachniet, Jeff; Laget, Jean; Langheinrich, Jorn; Lawrence, David; Li, Ji; Livingston, Kenneth; Lu, Haiyun; MacCormick, Marion; Markov, Nikolai; Mattione, Paul; McAleer, Simeon; McKinnon, Bryan; McNabb, John; Mecking, Bernhard; Mehrabyan, Surik; Melone, Joseph; Mestayer, Mac; Meyer, Curtis; Mibe, Tsutomu; Mikhaylov, Konstantin; Minehart, Ralph; Mirazita, Marco; Miskimen, Rory; Mokeev, Viktor; Moriya, Kei; Morrow, Steven; Moteabbed, Maryam; Mueller, James; Munevar Espitia, Edwin; Mutchler, Gordon; Nadel-Turonski, Pawel; Niccolai, Silvia; Niculescu, Gabriel; Niculescu, Maria-Ioana; Niczyporuk, Bogdan; Niroula, Megh; Niyazov, Rustam; Nozar, Mina; Osipenko, Mikhail; Ostrovidov, Alexander; Park, Kijun; Pasyuk, Evgueni; Paterson, Craig; Pereira, Sergio; Pierce, Joshua; Pivnyuk, Nikolay; Pocanic, Dinko; Pogorelko, Oleg; Pozdnyakov, Sergey; Preedom, Barry; Price, John; Prok, Yelena; Protopopescu, Dan; Raue, Brian; Riccardi, Gregory; Ricco, Giovanni; Ripani, Marco; Ritchie, Barry; Ronchetti, Federico; Rosner, Guenther; Rossi, Patrizia; Sabatie, Franck; Salamanca, Julian; Salgado, Carlos; Santoro, Joseph; Sapunenko, Vladimir; Schumacher, Reinhard; Serov, Vladimir; Sharabian, Youri; Sharov, Dmitri; Shvedunov, Nikolay; Smith, Elton; Smith, Lee; Sober, Daniel; Sokhan, Daria; Stavinsky, Aleksey; Stepanyan, Stepan; Stepanyan, Samuel; Stokes, Burnham; Stoler, Paul; Strakovski, Igor; Strauch, Steffen; Taiuti, Mauro; Tedeschi, David; Tkabladze, Avtandil; Tkachenko, Svyatoslav; Todor, Luminita; Ungaro, Maurizio; Vineyard, Michael; Vlassov, Alexander; Watts, Daniel; Weinstein, Lawrence; Williams, Michael; Wolin, Elliott; Yegneswaran, Amrit; Zana, Lorenzo; Zhang, Bin; Zhang, Jixie; Zhao, Bo; Zhao, Zhiwen

    2008-07-01

    The light vector mesons ($\\rho$, $\\omega$, and $\\phi$) were produced in deuterium, carbon, titanium, and iron targets in a search for possible in-medium modifications to the properties of the $\\rho$ meson at normal nuclear densities and zero temperature. The vector mesons were detected with the CEBAF Large Acceptance Spectrometer (CLAS) via their decays to $e^{+}e^{-}$. The rare leptonic decay was chosen to reduce final-state interactions. A combinatorial background was subtracted from the invariant mass spectra using a well-established event-mixing technique. The $\\rho$ meson mass spectrum was extracted after the $\\omega$ and $\\phi$ signals were removed in a nearly model-independent way. Comparisons were made between the $\\rho$ mass spectra from the heavy targets ($A > 2$) with the mass spectrum extracted from the deuterium target. With respect to the $\\rho$-meson mass, we obtain a small shift compatible with zero. Also, we measure widths consistent with standard nuclear many-body eff

  3. Casimir stress in an inhomogeneous medium

    International Nuclear Information System (INIS)

    Philbin, T.G.; Xiong, C.; Leonhardt, U.

    2010-01-01

    The Casimir effect in an inhomogeneous dielectric is investigated using Lifshitz's theory of electromagnetic vacuum energy. A permittivity function that depends continuously on one Cartesian coordinate is chosen, bounded on each side by homogeneous dielectrics. The result for the Casimir stress is infinite everywhere inside the inhomogeneous region, a divergence that does not occur for piece-wise homogeneous dielectrics with planar boundaries. A Casimir force per unit volume can be extracted from the infinite stress but it diverges on the boundaries between the inhomogeneous medium and the homogeneous dielectrics. An alternative regularization of the vacuum stress is considered that removes the contribution of the inhomogeneity over small distances, where macroscopic electromagnetism is invalid. The alternative regularization yields a finite Casimir stress inside the inhomogeneous region, but the stress and force per unit volume diverge on the boundaries with the homogeneous dielectrics. The case of inhomogeneous dielectrics with planar boundaries thus falls outside the current understanding of the Casimir effect.

  4. A medium resolution fingerprint matching system

    Directory of Open Access Journals (Sweden)

    Ayman Mohammad Bahaa-Eldin

    2013-09-01

    Full Text Available In this paper, a novel minutiae based fingerprint matching system is proposed. The system is suitable for medium resolution fingerprint images obtained by low cost commercial sensors. The paper presents a new thinning algorithm, a new features extraction and representation, and a novel feature distance matching algorithm. The proposed system is rotation and translation invariant and is suitable for complete or partial fingerprint matching. The proposed algorithms are optimized to be executed on low resource environments both in CPU power and memory space. The system was evaluated using a standard fingerprint dataset and good performance and accuracy were achieved under certain image quality requirements. In addition, the proposed system was compared favorably to that of the state of the art systems.

  5. CAS medium-size nuclear plants

    International Nuclear Information System (INIS)

    Vogelweith, L.; Weiss, A.

    1977-01-01

    CEA has developed a range of pressurized water reactors of the type CAS Compact, which are adapted to civil ship propulsion, or to electric power production, combined possibly with heat production, up to outputs equivalent to 125MW(e). Nuclear plants equipped with these reactors are suitable for medium-size electric networks, especially in developing countries, because they are easily adaptable, owing to their flexibility; they can be installed and used in a variety of ways (on land, floating installation, combination of electric power and other production, etc.); they can be used as training reactors by countries wishing to limit their investment plans before undertaking a wider nuclear development. Examples of two possible realizations are presented: as a floating plant, and as a combined electric and desalting plant. (author)

  6. Gravitational instability in a multicomponent expanding medium

    International Nuclear Information System (INIS)

    Solov'eva, L.V.; Nurgaliev, I.S.

    1985-01-01

    In the Newtonian approximation we consider the gravitational instability of a two- or N-component medium in an expanding universe. The system of density-perturbation equations is solved in the short- and long-wave limits. For small values of the wave vector k, a result obtained for the stationary case continues to hold true: at most there can exist only one unstable mode. If k is kept fixed, the introduction of a perturbation component delta/sub i/ will speed the growth of fluctuations delta/sub j/, provided the adiabatic indices γ/sub i/>γ/sub j/. In the large-k limit, ordinary acoustic waves result. Other components will begin to manifest themselves in the first-order terms when the oscillation amplitude is expanded in powers of k -1 : provided γ/sub j/>γ/sub i/> or =4/3, the ith-component amplitude will decay more slowly than otherwise

  7. Medium wave exposure characterisation using exposure quotients.

    Science.gov (United States)

    Paniagua, Jesús M; Rufo, Montaña; Jiménez, Antonio; Antolín, Alicia; Pinar, Iván

    2010-06-01

    One of the aspects considered in the International Commission on Non-Ionizing Radiation Protection guidelines is that, in situations of simultaneous exposure to fields of different frequencies, exposure quotients for thermal and electrical stimulation effects should be examined. The aim of the present work was to analyse the electromagnetic radiation levels and exposure quotients for exposure to multiple-frequency sources in the vicinity of medium wave radio broadcasting antennas. The measurements were made with a spectrum analyser and a monopole antenna. Kriging interpolation was used to prepare contour maps and to estimate the levels in the towns and villages of the zone. The results showed that the exposure quotient criterion based on electrical stimulation effects to be more stringent than those based on thermal effects or power density levels. Improvement of dosimetry evaluations requires the spectral components of the radiation to be quantified, followed by application of the criteria for exposure to multiple-frequency sources.

  8. Magnetic Fields in the Interstellar Medium

    Science.gov (United States)

    Clark, Susan

    2017-01-01

    The Milky Way is magnetized. Invisible magnetic fields thread the Galaxy on all scales and play a vital but still poorly understood role in regulating flows of gas in the interstellar medium and the formation of stars. I will present highlights from my thesis work on magnetic fields in the diffuse interstellar gas and in accretion disks. At high Galactic latitudes, diffuse neutral hydrogen is organized into an intricate network of slender linear features. I will show that these neutral hydrogen “fibers” are extremely well aligned with the ambient magnetic field as traced by both starlight polarization (Clark et al. 2014) and Planck 353 GHz polarized dust emission (Clark et al. 2015). The structure of the neutral interstellar medium is more tightly coupled to the magnetic field than previously known. Because the orientation of neutral hydrogen is an independent predictor of the local dust polarization angle, our work provides a new tool in the search for inflationary gravitational wave B-mode polarization in the cosmic microwave background, which is currently limited by dust foreground contamination. Magnetic fields also drive accretion in astrophysical disks via the magnetorotational instability (MRI). I analytically derive the behavior of this instability in the weakly nonlinear regime and show that the saturated state of the instability depends on the geometry of the background magnetic field. The analytical model describes the behavior of the MRI in a Taylor-Couette flow, a set-up used by experimentalists in the ongoing quest to observe MRI in the laboratory (Clark & Oishi 2016a, 2016b).

  9. BIFIDUM-MEDIUM FOR ISOLATION AND CULTIVATION OF BIFIDOBACTERIA

    Directory of Open Access Journals (Sweden)

    L. V. Domotenko

    2014-01-01

    Full Text Available The comparative evaluation of Bifidum-medium quality (the nutrient medium for cultivation and selection bifidobacteria, dry and Blaurock medium has been performed. It was shown that Bifidum-medium supports the typical growth of the main types of bifidobacteria: Bifidobacterium bifidum, B. breve, B. adolescentis, B. infantis, B. longum. The comparable results for the efficacy and the activity accumulation of acid were obtained in the study of probiotic «bifidumbacterin » using both media. In studies of faecal material the medium concentration of bifidobacteria was found 8.15 lg CFU/g on Bifidum-medium and 6.68 lg CFU/g on Blaurock medium.

  10. iBook – a New Medium?

    Directory of Open Access Journals (Sweden)

    Benjamin Štular

    2013-09-01

    Full Text Available EXTENDED ABSTRACT:On January 19th 2012 Apple launched iBooks®2 for iPad® - a new application for downloading digital content to the iPad - and the new software (iBooks Author® which allows creating and publishing digital content using the .iba digital format and all-new iTunes® application offering access to a variety of digitised educational content. Our aim was to test the attractiveness of the new Apple releases and the effect of a relatively easy access to the world market of digital content on the promotion of Slovenian cultural heritage. We added three books to the iTunes digital collection which might be of interest to the general public: a re-edition of a scientific monograph by Andrej Pleterski: Mythical Reality of the Carintian Dukes' Stones (Mitska stvarnost knežjega kamna in Slovenian and German version and a non-fiction iBook by Benjamin Štular: Ljubljana in the Middle Ages. Archaeological Research (Srednjeveška Ljubljana. Arheološke raziskave. The digital version of the Andrej Pleterski's book was chosen for the actuality of the topic in Slovenia and Austria. People and politicians in Carinthia were annoyed by the book contents as it was published by the Regional Museum in Klagenfurt in December 2011. While the science in this book is sound it has been abused to meet the current needs of some of the Carinthian politicians. The incident was polemically reflected in the main Slovenian newspapers, too. The non-fiction iBook was selected because of the attractiveness of the theme, besides a lot of multimedia content had already been created and could be easily published to Apple's iBookstore. All three iBooks were posted to iTunes® less than three weeks after the release of the iBooks Author application to create digital content. We wish to present our experience with the applicability of the new medium, users' feedback and access statistics. Guidelines for Apple licenses are presented. The applicability of the new medium and

  11. Effective Medium Theory for Anisotropic Metamaterials

    KAUST Repository

    Zhang, Xiujuan

    2017-11-12

    This dissertation includes the study of effective medium theories (EMTs) and their applications in describing wave propagation in anisotropic metamaterials, which can guide the design of metamaterials. An EMT based on field averaging is proposed to describe a peculiar anisotropic dispersion relation that is linear along the symmetry line but quadratic in the perpendicular direction. This dispersion relation is associated with the topological transition of the iso-frequency contours (IFCs), suggesting interesting wave propagation behaviors from beam shaping to beam splitting. In the framework of coherent potential approximation, an analytical EMT is further developed, with the ability to build a direct connection between the microscopic structure and the macroscopic material properties, which overcomes the requirement of prior knowledge of the field distributions. The derived EMT is valid beyond the long-wavelength limit. Using the EMT, an anisotropic zero-index metamaterial is designed. Moreover, the derived EMT imposes a condition that no scattered wave is generated in the ambient medium, which suggests the input signal cannot detect any object that might exist, making it invisible. Such correspondence between the EMT and the invisibilityinspires us to explore the wave cloaking in the same framework of coherent potential approximation. To further broaden the application realm of EMT, an EMT using the parameter retrieval method is studied in the regimes where the previously-developed EMTs are no longer accurate. Based on this study, in conjunction with the EMT mentioned above, a general scheme to realize coherent perfect absorption (CPA) in anisotropic metamaterials is proposed. As an exciting area in metamaterials, the field of metasurfaces has drawn great attention recently. As an easily attainable device, a grating may be the simplest version of metasurfaces. Here, an analytical EMT for gratings made of cylinders is developed by using the multiple scattering

  12. Commercial cinema: a medium for development communication.

    Science.gov (United States)

    Riber, J; Smith, S

    1985-01-01

    concerns about family planning. Understanding audience expectations in a commercial cinema context is the key to successfully adapting this medium for social development messages. Although some foreign technical assistance was provided, the success of the 2 films described depended on how their themes were adapted to the traditional feature film format. To date, commercial cinema has been an underutilized medium for development messages. WIF's experiences demonstrate how effective, well-conceived social messages can be presented in a commercial cinema context. It is hoped this will encourage other to use this widely available channel of mass communication.

  13. IMPLEMENTING SMALL AND MEDIUM IT PROJECTS IN SMALL AND MEDIUM ENERPRISES

    Directory of Open Access Journals (Sweden)

    Felix DUMITRESCU

    2014-06-01

    Full Text Available Information technology is essential nowadays for all companies. Small enterprises are an important part of the economy and this article aims at providing some useful insight in implementing modern IT projects to their benefit. Due to the limited funding available for the IT infrastructure in most start-ups and small businesses, the projects should be adapted to fulfill the needs of the company for the lowest cost. The paper will start by defining small and medium project management theory and outlining the target of the study, small and medium sized companies. Next it will show a number of case studies of IT projects implemented in different types of small companies in Romania. Based on these implementations the article will draw some conclusions relevant to most small companies which need to design or improve their IT infrastructure.

  14. Effects of egg white protein supplementation on muscle strength and serum free amino acid concentrations.

    Science.gov (United States)

    Hida, Azumi; Hasegawa, Yuko; Mekata, Yuko; Usuda, Mika; Masuda, Yasunobu; Kawano, Hitoshi; Kawano, Yukari

    2012-10-19

    The aim of this study was to evaluate the effects of egg white protein compared to carbohydrate intake prior to exercise on fat free mass (FFM), one repetition maximum (1RM) muscle strength and blood biochemistry in female athletes. Thirty healthy female collegiate athletes were recruited for this study and matched by sport type, body fat percentage and 1RM leg curl muscle strength. Participants were randomly divided into two groups: protein group (15.0 g egg white protein; 75 kcal) and carbohydrate group (17.5 g maltodextrin, 78 kcal). Supplements were administered daily at the same time in a double-blind manner prior to training during an 8-week period. Measurements were performed before and after the 8-week regimen. The mean dietary energy intake did not change throughout the study period. FFM and 1RM assessments (i.e., leg curl, leg extension, squat, and bench press) increased in both groups. Furthermore, serum urea and serum citrulline levels after the 8-week regimen increased significantly only in the protein group. Our findings indicated that compared to the carbohydrate supplement, the protein supplement was associated with some changes in protein metabolites but not with changes in body composition or muscle strength.

  15. Effects of Egg White Protein Supplementation on Muscle Strength and Serum Free Amino Acid Concentrations

    Directory of Open Access Journals (Sweden)

    Yukari Kawano

    2012-10-01

    Full Text Available The aim of this study was to evaluate the effects of egg white protein compared to carbohydrate intake prior to exercise on fat free mass (FFM, one repetition maximum (1RM muscle strength and blood biochemistry in female athletes. Thirty healthy female collegiate athletes were recruited for this study and matched by sport type, body fat percentage and 1RM leg curl muscle strength. Participants were randomly divided into two groups: protein group (15.0 g egg white protein; 75 kcal and carbohydrate group (17.5 g maltodextrin, 78 kcal. Supplements were administered daily at the same time in a double-blind manner prior to training during an 8-week period. Measurements were performed before and after the 8-week regimen. The mean dietary energy intake did not change throughout the study period. FFM and 1RM assessments (i.e., leg curl, leg extension, squat, and bench press increased in both groups. Furthermore, serum urea and serum citrulline levels after the 8-week regimen increased significantly only in the protein group. Our findings indicated that compared to the carbohydrate supplement, the protein supplement was associated with some changes in protein metabolites but not with changes in body composition or muscle strength.

  16. Effects of Egg White Protein Supplementation on Muscle Strength and Serum Free Amino Acid Concentrations

    Science.gov (United States)

    Hida, Azumi; Hasegawa, Yuko; Mekata, Yuko; Usuda, Mika; Masuda, Yasunobu; Kawano, Hitoshi; Kawano, Yukari

    2012-01-01

    The aim of this study was to evaluate the effects of egg white protein compared to carbohydrate intake prior to exercise on fat free mass (FFM), one repetition maximum (1RM) muscle strength and blood biochemistry in female athletes. Thirty healthy female collegiate athletes were recruited for this study and matched by sport type, body fat percentage and 1RM leg curl muscle strength. Participants were randomly divided into two groups: protein group (15.0 g egg white protein; 75 kcal) and carbohydrate group (17.5 g maltodextrin, 78 kcal). Supplements were administered daily at the same time in a double-blind manner prior to training during an 8-week period. Measurements were performed before and after the 8-week regimen. The mean dietary energy intake did not change throughout the study period. FFM and 1RM assessments (i.e., leg curl, leg extension, squat, and bench press) increased in both groups. Furthermore, serum urea and serum citrulline levels after the 8-week regimen increased significantly only in the protein group. Our findings indicated that compared to the carbohydrate supplement, the protein supplement was associated with some changes in protein metabolites but not with changes in body composition or muscle strength. PMID:23201768

  17. Breast cancer proliferative activity: Is it the source of serum free DNA?

    African Journals Online (AJOL)

    Taha I. Hewala

    2013-04-13

    Apr 13, 2013 ... DNA concentration in lung cancer patients was 8-fold higher ... patients with invasive ductal carcinoma of clinical stages II and III,12 recently ... centrifuged for 20 min at 3500 rpm at 4 °C. The separated ser- um samples were ...

  18. Clinical significance of the measurements of serum free thyroxine and free triiodothyronine concentrations

    International Nuclear Information System (INIS)

    Kubota, Ken; Sasaki, Norio; Takaku, Fumimaro; Uchimura, Hidemasa

    1988-01-01

    A commercially available ''DPC'' radioimmunoassay kit was used to study the serum concentrations of free triiodothyronine (FT3) and free thyroxine (FT4) in a series of 189 patients with various thyroid diseases and 120 healthy controls. The basal serum concentrations of FT3 and FT4 in normal controls ranged from 0.98 to 2.04 ng/dl and from 1.43 to 3.66 pg/ml, respectively. All untreated patients with Graves' disease had abnormally high FT3 and FT4 values, indicating the discrimination between hyperthyroid and normal subjects. A decreased ratio of FT4 to FT3 was observed in patients managed with antithyroid drugs. In diagnosing hypothyroidism, ''DPC'' FT4 kits were more sensitive than ''DPC'' TT4 and ''Amerlex'' FT4 kits. In the case of non-thyroid diseases, FT4 and FT3 values, as well as total T4 and total T3, were normal or decreased, with the exception of occasionally unknown high values. The interference of thyroxine binding globulin abnormablity was successfully eliminated by using new versions of ''DPC'' kits. (Namekawa, K.)

  19. Serum free amino acid concentration in hepatic lipidosis of dairy cows in the periparturient period.

    Science.gov (United States)

    Shibano, Ken-ichi; Kawamura, Seiichi

    2006-04-01

    Blood samples were taken from eight multiparous cows at a dairy farm on eight occasions between the prepartum period and peak lactation to study the serum concentrations of amino acids and biochemical constituents. The cows were classified as having either severe hepatic lipidosis (HL) or non-hepatic lipidosis (non-HL) according to their clinical condition after calving and changes in serum biochemical parameters. The serum concentrations of beta-hydroxybutyric acid were higher in the HL group than in the non-HL group (ANOVA: phepatic lipidosis.

  20. Effects of a honeybee sting on the serum free amino acid profile in humans.

    Directory of Open Access Journals (Sweden)

    Jan Matysiak

    Full Text Available The aim of this study was to assess the response to a honeybee venom by analyzing serum levels of 34 free amino acids. Another goal of this study was to apply complex analytic-bioinformatic-clinical strategy based on up-to-date achievements of mass spectrometry in metabolomic profiling. The amino acid profiles were determined using hybrid triple quadrupole/linear ion trap mass spectrometer coupled with a liquid chromatography instrument. Serum samples were collected from 27 beekeepers within 3 hours after they were stung and after a minimum of 6 weeks following the last sting. The differences in amino acid profiles were evaluated using MetaboAnalyst and ROCCET web portals. Chemometric tests showed statistically significant differences in the levels of L-glutamine (Gln, L-glutamic acid (Glu, L-methionine (Met and 3-methyl-L-histidine (3MHis between the two analyzed groups of serum samples. Gln and Glu appeared to be the most important metabolites for distinguishing the beekeepers tested shortly after a bee sting from those tested at least 6 weeks later. The role of some amino acids in the response of an organism to the honeybee sting was also discussed. This study indicated that proposed methodology may allow to identify the individuals just after the sting and those who were stung at least 6 weeks earlier. The results we obtained will contribute to better understanding of the human body response to the honeybee sting.

  1. Growth Hormone Supplementation in the Luteal Phase Before Microdose GnRH Agonist Flare Protocol for In Vitro Fertilization.

    Science.gov (United States)

    Dunne, Caitlin; Seethram, Ken; Roberts, Jeffrey

    2015-09-01

    Growth hormone (GH) acts in both early and late follicular development to stimulate the proliferation and differentiation of granulosa cells and to increase the production of estradiol in animal and human ovaries. Investigators have therefore explored GH supplementation to improve outcomes in women undergoing in vitro fertilization, with the greatest interest in women with diminished ovarian reserve. Recent meta-analyses indicate that GH supplementation can be beneficial for poor responders undergoing IVF. In most studies, GH has been given concomitantly with gonadotropins during the follicular phase; this may not be optimal, since follicular recruitment begins during the preceding luteal phase. We therefore wished to examine the effect of GH supplementation in the luteal phase before controlled ovarian stimulation (COH) with a microdose GnRH agonist flare (MDF) protocol in women undergoing in vitro fertilization. We performed a retrospective matched case-control study of patients undergoing treatment at a private IVF facility between June 2012 and July 2013. Patients identified as poor responders to COH were offered adjuvant GH treatment as part of their ovarian stimulation regimen. The patients in the experimental group chose to take GH, 3.33 mg daily by subcutaneous injection for 14 days, before starting COH. All patients had an MDF stimulation protocol using 450 IU of follicle stimulating hormone (FSH) daily. A total of 42 women were included in the study. There were 14 women in the experimental group (GH) and 28 controls (C) matched for age, BMI, and day 3 FSH level. There was no difference between the groups in clinical pregnancy rate (GH = 29%, C = 32%, P = 0.99), number of mature oocytes retrieved (GH = 2.5, C = 5.0, P = 0.13), cycle cancellation rate (GH = 21%, C = 14%, P = 0.88), duration of COH (GH = 10.1, C = 10.1, P = 0.93), or mean peak estradiol level (GH = 4174 pmol/L, C = 5105 pmol/L, P = 0.44). The administration of growth hormone during the luteal phase before a microdose GnRH agonist flare protocol for in vitro fertilization did not improve outcomes in "poor responder" patients.

  2. ATMEA and medium power reactors. The ATMEA joint venture and the ATMEA1 medium power reactor

    International Nuclear Information System (INIS)

    Mathet, Eric; Castello, Gerard

    2012-01-01

    This Power Point presentation presents the ATMEA company (a joint venture of Areva and Mitsubishi), the main features of its medium power reactor (ATMEA1) and its building arrangement, indicates the general safety objectives. It outlines the features of its robust design which aim at protecting, cooling down and containing. It indicates the regulatory and safety frameworks, comments the review of the safety options by the ASN and the results of this assessment

  3. A note on the effective medium theory of random resistive-reactive medium

    International Nuclear Information System (INIS)

    Kumar, N.; Jayannavar, A.M.

    1981-06-01

    The effective medium theory for a system with randomly distributed point conductivity and polarizability is re-formulated, with attention to cross-terms involving the two disorder parameters. The treatment reveals a certain inconsistency of the conventional theory owing to the neglect of the Maxwell-Wagner effect. The results are significant for the critical resistivity and dielectric anomalies of a binary liquid mixture at the phase-separation point. (author)

  4. Evaluation of insulin medium or chondrogenic medium on proliferation and chondrogenesis of ATDC5 cells.

    Science.gov (United States)

    Yao, Yongchang; Zhai, Zhichen; Wang, Yingjun

    2014-01-01

    The ATDC5 cell line is regarded as an excellent cell model for chondrogenesis. In most studies with ATDC5 cells, insulin medium (IM) was used to induce chondrogenesis while chondrogenic medium (CM), which was usually applied in chondrogenesis of mesenchymal stem cells (MSCs), was rarely used for ATDC5 cells. This study was mainly designed to investigate the effect of IM, CM, and growth medium (GM) on chondrogenesis of ATDC5 cells. ATDC5 cells were, respectively, cultured in IM, CM, and GM for a certain time. Then the proliferation and the chondrogenesis progress of cells in these groups were analyzed. Compared with CM and GM, IM promoted the proliferation of cells significantly. CM was effective for enhancement of cartilage specific markers, while IM induced the cells to express endochondral ossification related genes. Although GAG deposition per cell in CM group was significantly higher than that in IM and GM groups, the total GAG contents in IM group were the most. This study demonstrated that CM focused on induction of chondrogenic differentiation while IM was in favor of promoting proliferation and expression of endochondral ossification related genes. Combinational use of these two media would be more beneficial to bone/cartilage repair.

  5. Evaluation of Insulin Medium or Chondrogenic Medium on Proliferation and Chondrogenesis of ATDC5 Cells

    Directory of Open Access Journals (Sweden)

    Yongchang Yao

    2014-01-01

    Full Text Available Background. The ATDC5 cell line is regarded as an excellent cell model for chondrogenesis. In most studies with ATDC5 cells, insulin medium (IM was used to induce chondrogenesis while chondrogenic medium (CM, which was usually applied in chondrogenesis of mesenchymal stem cells (MSCs, was rarely used for ATDC5 cells. This study was mainly designed to investigate the effect of IM, CM, and growth medium (GM on chondrogenesis of ATDC5 cells. Methods. ATDC5 cells were, respectively, cultured in IM, CM, and GM for a certain time. Then the proliferation and the chondrogenesis progress of cells in these groups were analyzed. Results. Compared with CM and GM, IM promoted the proliferation of cells significantly. CM was effective for enhancement of cartilage specific markers, while IM induced the cells to express endochondral ossification related genes. Although GAG deposition per cell in CM group was significantly higher than that in IM and GM groups, the total GAG contents in IM group were the most. Conclusion. This study demonstrated that CM focused on induction of chondrogenic differentiation while IM was in favor of promoting proliferation and expression of endochondral ossification related genes. Combinational use of these two media would be more beneficial to bone/cartilage repair.

  6. Degradation testing of Mg alloys in Dulbecco's modified eagle medium: Influence of medium sterilization

    International Nuclear Information System (INIS)

    Marco, Iñigo; Feyerabend, Frank; Willumeit-Römer, Regine; Van der Biest, Omer

    2016-01-01

    This work studies the in vitro degradation of Mg alloys for bioabsorbable implant applications under near physiological conditions. For this purpose, the degradation behaviour of Mg alloys in Dulbecco's modified eagle medium (DMEM) which is a commonly used cell culture medium is analysed. Unfortunately, DMEM can be contaminated by microorganisms, acidifying the medium and accelerating the Mg degradation process by dissolution of protective degradation layers, such as (Mg_x,Ca_y)(PO_4)_z. In this paper the influence of sterilization by applying UV-C radiation and antibiotics (penicillin/streptomycin) is analysed with two implant material candidates: Mg–Gd and Mg–Ag alloys; and pure magnesium as well as Mg–4Y–3RE as a reference. - Highlights: • Contamination of DMEM by microorganisms increases the degradation rate of Mg. • Mg and its alloys show passivation during long term immersion tests in DMEM. • The use of a control sample position is essential to assess H_2 evolution in DMEM.

  7. Microscopic optical potential at medium energies

    International Nuclear Information System (INIS)

    Malecki, A.

    1979-01-01

    The problems concerning a microscopic optical model for the elastic nuclear collisions at medium energies are discussed. We describe the method for constructing the optical potential which makes use of the particular properties of quantum scattering in the eikonal limit. The resulting potential is expressed in terms of the nuclear wave functions and the nucleon-nucleon scattering amplitudes. This potential has a dynamic character since by including the effects of multiple scattering it allows for the possibility of intermediate excitations of the projectile and target nuclei. The use of the potential in the exact wave equation accounts for the most important mechanisms present in the collisions between composite particles. The microscopic optical model was successfully applied in the analysis of elastic scattering of protons and α-particles on atomic nuclei in the energy range of 300-1000 MeV/nucleon. The dynamic optical potential in this case represents a considerable improvement over the eikonal Glauber model and the static optical potential of Watson. The possibilities to extend the microscopic description of the proton-nucleus interaction by considering the spin dependence of the elementary amplitude and the Majorana exchange effects were investigated. (author)

  8. SILICATE COMPOSITION OF THE INTERSTELLAR MEDIUM

    Energy Technology Data Exchange (ETDEWEB)

    Fogerty, S.; Forrest, W.; Watson, D. M.; Koch, I. [Department of Physics and Astronomy, University of Rochester, Rochester, NY 14627 (United States); Sargent, B. A., E-mail: sfogerty@pas.rochester.edu [Center for Imaging Science and Laboratory for Multiwavelength Astrophysics, Rochester Institute of Technology, 54 Lomb Memorial Drive, Rochester, NY 14623 (United States)

    2016-10-20

    The composition of silicate dust in the diffuse interstellar medium and in protoplanetary disks around young stars informs our understanding of the processing and evolution of the dust grains leading up to planet formation. An analysis of the well-known 9.7 μ m feature indicates that small amorphous silicate grains represent a significant fraction of interstellar dust and are also major components of protoplanetary disks. However, this feature is typically modeled assuming amorphous silicate dust of olivine and pyroxene stoichiometries. Here, we analyze interstellar dust with models of silicate dust that include non-stoichiometric amorphous silicate grains. Modeling the optical depth along lines of sight toward the extinguished objects Cyg OB2 No. 12 and ζ Ophiuchi, we find evidence for interstellar amorphous silicate dust with stoichiometry intermediate between olivine and pyroxene, which we simply refer to as “polivene.” Finally, we compare these results to models of silicate emission from the Trapezium and protoplanetary disks in Taurus.

  9. UV observations of local interstellar medium.

    Science.gov (United States)

    Kurt, V.; Mironova, E.; Fadeev, E.

    2008-12-01

    The methods of the interstellar matter study are described. The brief information of space missions aimed at observations in the unreachable for ground based telescopes UV spectral range (IUE, As- tron, HST and GALEX.) is presented. The history of discovery of H and He atoms entering the Solar System from the local interstellar medium (LISM) is given in brief. The results of observations performed by the group from Stern- berg Astronomical Institute (SAI MSU) and Space Research Institute (IKI RAS) performed with the help of the missions Prognoz-5, Prognoz-6 and the stations Zond-1, Venera and Mars and aimed at estimation of all basic LISM parameters (the velocity of the Sun in relation to LISM, directions of movement, densities of H and He atoms, LISM temperature) are presented. We also describe the present-day investigations of LISM performed with SOHO and ULYSSES mis- sions including the direct registration of He atoms entering the Solar System. The problem of interaction between the incoming flow of the ISM atoms ("in- terstellar wind") and the area of two shocks at the heliopause border (100-200 AU) is discussed. The LISM parameters obtained using the available data are presented in two tables.

  10. SILICATE COMPOSITION OF THE INTERSTELLAR MEDIUM

    International Nuclear Information System (INIS)

    Fogerty, S.; Forrest, W.; Watson, D. M.; Koch, I.; Sargent, B. A.

    2016-01-01

    The composition of silicate dust in the diffuse interstellar medium and in protoplanetary disks around young stars informs our understanding of the processing and evolution of the dust grains leading up to planet formation. An analysis of the well-known 9.7 μ m feature indicates that small amorphous silicate grains represent a significant fraction of interstellar dust and are also major components of protoplanetary disks. However, this feature is typically modeled assuming amorphous silicate dust of olivine and pyroxene stoichiometries. Here, we analyze interstellar dust with models of silicate dust that include non-stoichiometric amorphous silicate grains. Modeling the optical depth along lines of sight toward the extinguished objects Cyg OB2 No. 12 and ζ Ophiuchi, we find evidence for interstellar amorphous silicate dust with stoichiometry intermediate between olivine and pyroxene, which we simply refer to as “polivene.” Finally, we compare these results to models of silicate emission from the Trapezium and protoplanetary disks in Taurus.

  11. Medium Deep High Temperature Heat Storage

    Science.gov (United States)

    Bär, Kristian; Rühaak, Wolfram; Schulte, Daniel; Welsch, Bastian; Chauhan, Swarup; Homuth, Sebastian; Sass, Ingo

    2015-04-01

    Heating of buildings requires more than 25 % of the total end energy consumption in Germany. Shallow geothermal systems for indirect use as well as shallow geothermal heat storage systems like aquifer thermal energy storage (ATES) or borehole thermal energy storage (BTES) typically provide low exergy heat. The temperature levels and ranges typically require a coupling with heat pumps. By storing hot water from solar panels or thermal power stations with temperatures of up to 110 °C a medium deep high temperature heat storage (MDHTS) can be operated on relatively high temperature levels of more than 45 °C. Storage depths of 500 m to 1,500 m below surface avoid conflicts with groundwater use for drinking water or other purposes. Permeability is typically also decreasing with greater depth; especially in the crystalline basement therefore conduction becomes the dominant heat transport process. Solar-thermal charging of a MDHTS is a very beneficial option for supplying heat in urban and rural systems. Feasibility and design criteria of different system configurations (depth, distance and number of BHE) are discussed. One system is designed to store and supply heat (300 kW) for an office building. The required boreholes are located in granodioritic bedrock. Resulting from this setup several challenges have to be addressed. The drilling and completion has to be planned carefully under consideration of the geological and tectonical situation at the specific site.

  12. A Multiphase Model for the Intracluster Medium

    Science.gov (United States)

    Nagai, Daisuke; Sulkanen, Martin E.; Evrard, August E.

    1999-01-01

    Constraints on the clustered mass density of the universe derived from the observed population mean intracluster gas fraction of x-ray clusters may be biased by reliance on a single-phase assumption for the thermodynamic structure of the intracluster medium (ICM). We propose a descriptive model for multiphase structure in which a spherically symmetric ICM contains isobaric density perturbations with a radially dependent variance. Fixing the x-ray emission and emission weighted temperature, we explore two independently observable signatures of the model in the parameter space. For bremsstrahlung dominated emission, the central Sunyaev-Zel'dovich (SZ) decrement in the multiphase case is increased over the single-phase case and multiphase x-ray spectra in the range 0.1-20 keV are flatter in the continuum and exhibit stronger low energy emission lines than their single-phase counterpart. We quantify these effects for a fiducial 10e8 K cluster and demonstrate how the combination of SZ and x-ray spectroscopy can be used to identify a preferred location in the plane of the model parameter space. From these parameters the correct value of mean intracluster gas fraction in the multiphase model results, allowing an unbiased estimate of clustered mass density to he recovered.

  13. From medium-sized to megawatt turbines...

    Energy Technology Data Exchange (ETDEWEB)

    Dongen, W. van [NedWind bv, Rhenen (Netherlands)

    1996-12-31

    One of the world`s first 500 kW turbines was installed in 1989 in the Netherlands. This forerunner of the current NedWind 500 kW range also represents the earliest predesign of the NedWind megawatt turbine. After the first 500 kW turbines with steel rotor blades and rotor diameter of 34 m, several design modifications followed, e.g. the rotor diameter was increased to 35 m and a tip brake was added. Later polyester blades were introduced and the rotor diameter was increased with 5 in. The drive train was also redesigned. Improvements on the 500 kW turbine concept has resulted in decreased cost, whereas annual energy output has increased to approx. 1.3 million kWh. Wind energy can substantially contribute to electricity supply. Maximum output in kiloWatthours is the target. Further improvement of the existing technology and implementation of flexible components may well prove to be a way to increase energy output, not only in medium or large sized wind turbines. 7 figs.

  14. On Graphene in the Interstellar Medium

    Science.gov (United States)

    Chen, X. H.; Li, Aigen; Zhang, Ke

    2017-11-01

    The possible detection of C24, a planar graphene that was recently reported to be in several planetary nebulae by García-Hernández et al., inspires us to explore whether and how much graphene could exist in the interstellar medium (ISM) and how it would reveal its presence through its ultraviolet (UV) extinction and infrared (IR) emission. In principle, interstellar graphene could arise from the photochemical processing of polycyclic aromatic hydrocarbon (PAH) molecules, which are abundant in the ISM, due to the complete loss of their hydrogen atoms, and/or from graphite, which is thought to be a major dust species in the ISM, via fragmentation caused by grain–grain collisional shattering. Both quantum-chemical computations and laboratory experiments have shown that the exciton-dominated electronic transitions in graphene cause a strong absorption band near 2755 \\mathringA . We calculate the UV absorption of graphene and place an upper limit of ∼5 ppm of C/H (i.e., ∼1.9% of the total interstellar C) on the interstellar graphene abundance. We also model the stochastic heating of graphene C24 in the ISM, excited by single starlight photons of the interstellar radiation field and calculate its IR emission spectra. We also derive the abundance of graphene in the ISM to be <5 ppm of C/H by comparing the model emission spectra with that observed in the ISM.

  15. The physical state of the intergalactic medium

    International Nuclear Information System (INIS)

    Barcons, X.; Fabian, A.C.; Rees, M.J.

    1991-01-01

    Because the process of galaxy formation is most unlikely to be perfectly efficient, there is a strong possibility that some baryonic gas remains outside collapsed structures such as galaxies and clusters of galaxies. What fraction of the baryonic content of the Universe resides in this intergalactic medium (IGM) and what physical state it is in are open questions. Here we use observational limits on the density of neutral hydrogen in the IGM, on the lack of deviations from a black-body spectrum of the cosmic microwave background (MBR), and on the extragalactic component of the soft X-ray background (XRB) to constrain the state of the IGM. From the lack of MBR fluctuations, any energetic IGM (containing as much energy as the binding energy in galaxies) is inferred to be smoothly distributed on scales greater than galactic. This rules out hot IGM models for the origin of the hard X-ray background, as well as the hypothesis that cosmic explosions may have given rise to cosmological structure on scales larger than galaxies. (author)

  16. Innovative Characteristics of Small and Medium Enterprises

    Directory of Open Access Journals (Sweden)

    Barbara Bigliardi

    2011-06-01

    Full Text Available The purpose of the paper is to investigate the technological innovative characteristics in the Small and Medium Enterprises (SMEs belonging to the Italian manufacturing sector. A survey based on e-mail and fax questionnaire of 285 SMEs was conducted with a response rate of about 45%. On the basis of this survey, the innovative profile of SMEs has been highlighted as well as the factors and problems of innovative process analyzed. The methodology consisted in a descriptive analysis on general data and in a discriminant analysis on data related to the innovative activity and aimed at determine the factors distinguishing more and less innovative companies. The results show that innovative firms are market anticipation and customer focus, point to product enrichment in terms of different characteristics in respect to competitors’ products in order to obtain a superior product in terms of quality. Innovation results to be part of their business strategy and is based more around developing new ways of working than new product innovations.

  17. Gamma rays from the interstellar medium

    International Nuclear Information System (INIS)

    Bloemen, J.B.G.M.

    1985-01-01

    This thesis describes new gamma-ray views on cosmic rays and the interstellar medium. The author describes the COS-B data base and the pre-launch and in-flight calibration data used for all analyses. Diffuse galactic gamma radiation (> 50 MeV) may be either a result of cosmic-ray-matter interactions, or of the cosmic-ray electrons with the interstellar radiation field (mainly at optical and infrared wavelengths), through the inverse-Compton process. A detailed comparison between the gamma-ray observations of the large complex of interstellar clouds in Orion and Monoceros and the CO and HI surveys of this region is given. It gives insight into the cloud penetration of cosmic rays and in the relation between CO detections and molecular hydrogen column densities. Next, the radial distribution of gamma rays in the Galaxy is studied, as well as the galactic centre (more precisely, the central 400 pc), which contains a large concentration of CO molecules. The H 2 /CO abundance and the cosmic-ray density in the galactic centre are discussed and compared to the findings for the galactic disk. In various analyses in this thesis a likelihood-ratio method is applied for parameter estimation and hypothesis testing. A general description of this method is added as an appendix. (Auth.)

  18. A selective and differential medium for Vibrio harveyi.

    Science.gov (United States)

    Harris, L; Owens, L; Smith, S

    1996-01-01

    A new medium, termed Vibrio harveyi agar, has been developed for the isolation and enumeration of V. harveyi. It is possible to differentiate V. harveyi colonies from the colonies of strains representing 15 other Vibrio species with this medium. This medium has been shown to inhibit the growth of two strains of marine Pseudomonas spp. and two strains of marine Flavobacterium spp. but to allow the growth of Photobacterium strains. Colonies displaying typical V. harveyi morphology were isolated from the larval rearing water of a commercial prawn hatchery with V. harveyi agar as a primary isolation medium and were positively identified, by conventional tests, as V. harveyi. This agar displays great potential as a primary isolation medium and offers significant advantages over thiosulfate-citrate-bile salts-sucrose agar as a medium for differentiating V. harveyi from other marine and estuarine Vibrio species. PMID:8795252

  19. The effects of protoype medium on usability testing.

    Science.gov (United States)

    Boothe, Chase; Strawderman, Lesley; Hosea, Ethan

    2013-11-01

    Inconsistencies among testing methods and results in previous research prompted this study that builds upon a systematic usability testing research framework to better understand how interface medium influences users' abilities to detect usability flaws in applications. Interface medium was tested to identify its effects on users' perceptions of usability and abilities to detect usability problems and severe usability problems. Results indicated that medium has no effect on users' abilities to detect usability problems or perceptions of usability. However, results did indicate an interaction between the medium and the tested application in which users were able to identify significantly more usability problems on a higher fidelity medium using a particular application. Results also indicated that as users' perceptions of an application's usability increases, the users are less able to detect usability problems in that application. Usability testing should begin early in the design process, even if low fidelity mediums will be used. Copyright © 2013 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  20. Effect of Acidity of a Medium on Riboflavin Photodestruction

    Science.gov (United States)

    Astanov, S. Kh.; Turdiev, M.; Sharipov, M. Z.; Kurtaliev, É. N.; Nizomov, N. N.

    2016-03-01

    Effect of acidity of a medium on the spectroscopic characteristics of riboflavin aqueous solutions is investigated by the method of fluorescent and absorption spectroscopy. Significant deformation of the electronic spectra of riboflavin aqueous solutions irradiated with unfiltered light of a PRK-2 lamp is observed. It is established that riboflavin photostability in an acid medium is about twice as much as the photostability in a neutral medium, which is caused by the formation of a protonated species.

  1. A selective and differential medium for Vibrio harveyi.

    OpenAIRE

    Harris, L; Owens, L; Smith, S

    1996-01-01

    A new medium, termed Vibrio harveyi agar, has been developed for the isolation and enumeration of V. harveyi. It is possible to differentiate V. harveyi colonies from the colonies of strains representing 15 other Vibrio species with this medium. This medium has been shown to inhibit the growth of two strains of marine Pseudomonas spp. and two strains of marine Flavobacterium spp. but to allow the growth of Photobacterium strains. Colonies displaying typical V. harveyi morphology were isolated...

  2. Dust in the Diffuse Neutral Interstellar Medium

    Science.gov (United States)

    Sofia, Ulysses J.

    2008-05-01

    Studies of interstellar dust have always relied heavily upon Laboratory Astrophysics for interpretation. Laboratory values, in the broad sense that includes theory, are needed for the most basic act of measuring interstellar abundances, to the more complex determination of what grains are responsible for particular extinction. The symbiotic relationship between astronomical observations and Laboratory Astrophysics has prompted both fields to move forward, especially in the era of high-resolution ultraviolet spectroscopy when new elemental species could be interpreted and observations were able to show the limits of laboratory determinations. Thanks to this synergy, we currently have a good idea of the quantity of the most abundant elements incorporated into dust in diffuse neutral interstellar clouds: carbon, oxygen, iron, silicon and magnesium. Now the task is to figure out how, chemically and physically, those elements are integrated into interstellar grains. We can do this by comparing extinction curves to grain populations in radiative transfer models. The limitation at the present time is the availability of optical constants in the infrared through ultraviolet for species that are likely to exist in dust, i.e., those that are easy to form in the physical environments around stars and in molecular clouds. Extinction in some lines of sight can be fit within current abundance limits and with the optical constants that are available. However the inability to reproduce other extinction curves suggests that optical constants can be improved, either in quality for compounds that have been measured, or quantity in the sense of providing data for more materials. This talk will address the current state and the future of dust studies in the diffuse neutral interstellar medium. This work is supported by the grant HST-AR-10979.01-A from the Space Telescope Science Institute to Whitman College.

  3. Development of the Medium Energy Linac Systems

    International Nuclear Information System (INIS)

    Jang, Ji Ho; Kwon, Hyeok Jung; Kim, Dae Il; Kim, Han Sung; Park, Bum Sik; Seol, Kyung Tae; Song, Young Gi; Yun, Sang Pil; Cho, Yong Sub; Hong, In Seok

    2008-05-01

    The main purpose of this project is developing 100-MeV proton linear accelerator (linac) for proton engineering frontier project (PEFP). In the first phase of the PEFP, the development of the 20-MeV linac has successfully finished. Hence the work scope of this project is designing the linac to accelerate proton beams from 20-MeV up to 100-MeV, fabricating the linac up to 45 MeV, fabricating one set of the medium energy beam transport (MEBT) tank, and developing the low level radio frequency (LLRF) system and the control system. The basic role of the new proton accelerator is accelerating 20-mA proton beams from 20 MeV up to 100 MeV. The first step of the design procedure is optimizing and determining the accelerator parameters. The beam loss is also main concern in the design stage. The drift tube (DT) and the quadrupole magnets are designed to be optimized to the new linac design. The other purpose is confirming the new design by fabricating and tuning the drift tube linac (DTL). The 20MeV proton beam divided into two directions. One is supplying the beams to user group by turning on the 45-degree bending magnet. The other is guided into the 100-MeV DTL by tuning off the dipole magnet. That is why the PEFP MEBT located after 20-MeV DTL. The MEBT is realized as two small DTL tanks with three cells and a 45-degree bending magnet. The fabrication of one MEBT tank is another purpose of this project. The other purposes of this project is developing the LLRF system to control the RF signal and control system to monitor and control the vacuum system, magnet power supply, etc

  4. Solid H2 in the interstellar medium

    Science.gov (United States)

    Füglistaler, A.; Pfenniger, D.

    2018-06-01

    Context. Condensation of H2 in the interstellar medium (ISM) has long been seen as a possibility, either by deposition on dust grains or thanks to a phase transition combined with self-gravity. H2 condensation might explain the observed low efficiency of star formation and might help to hide baryons in spiral galaxies. Aims: Our aim is to quantify the solid fraction of H2 in the ISM due to a phase transition including self-gravity for different densities and temperatures in order to use the results in more complex simulations of the ISM as subgrid physics. Methods: We used molecular dynamics simulations of fluids at different temperatures and densities to study the formation of solids. Once the simulations reached a steady state, we calculated the solid mass fraction, energy increase, and timescales. By determining the power laws measured over several orders of magnitude, we extrapolated to lower densities the higher density fluids that can be simulated with current computers. Results: The solid fraction and energy increase of fluids in a phase transition are above 0.1 and do not follow a power law. Fluids out of a phase transition are still forming a small amount of solids due to chance encounters of molecules. The solid mass fraction and energy increase of these fluids are linearly dependent on density and can easily be extrapolated. The timescale is below one second, the condensation can be considered instantaneous. Conclusions: The presence of solid H2 grains has important dynamic implications on the ISM as they may be the building blocks for larger solid bodies when gravity is included. We provide the solid mass fraction, energy increase, and timescales for high density fluids and extrapolation laws for lower densities.

  5. Hydrocarbons biodegradation in unsaturated porous medium

    International Nuclear Information System (INIS)

    Gautier, C.

    2007-12-01

    Biological processes are expected to play an important role in the degradation of petroleum hydrocarbons in contaminated soils. However, factors influencing the kinetics of biodegradation are still not well known, especially in the unsaturated zone. To address these biodegradation questions in the unsaturated zone an innovative experimental set up based on a physical column model was developed. This experimental set up appeared to be an excellent tool for elaboration of a structured porous medium, with well defined porous network and adjusted water/oil saturations. Homogeneous repartition of both liquid phases (i.e., aqueous and non aqueous) in the soil pores, which also contain air, was achieved using ceramic membranes placed at the bottom of the soil column. Reproducible interfaces (and connectivity) are developed between gas, and both non mobile water and NAPL phases, depending on the above-defined characteristics of the porous media and on the partial saturations of these three phases (NAPL, water and gas). A respirometric apparatus was coupled to the column. Such experimental set up have been validated with hexadecane in dilution in an HMN phase. This approach allowed detailed information concerning n-hexadecane biodegradation, in aerobic condition, through the profile of the oxygen consumption rate. We have taken benefit of this technique, varying experimental conditions, to determine the main parameters influencing the biodegradation kinetics and compositional evolution of hydrocarbons, under steady state unsaturated conditions and with respect to aerobic metabolism. Impacts of the nitrogen quantity and of three different grain sizes have been examined. Biodegradation of petroleum cut, as diesel cut and middle distillate without aromatic fraction, were, also studied. (author)

  6. Enclosed Small and Medium Caliber Firing Experimental Facility

    Data.gov (United States)

    Federal Laboratory Consortium — This facility conducts completely instrumented terminal ballistics experimental tests with small and medium-caliber tungsten alloy penetrators against advanced armor...

  7. Amniocar as a proliferative medium for mesenchymal cells

    Directory of Open Access Journals (Sweden)

    V. V. Chestkov

    2014-01-01

    Full Text Available Objectives. To develop the Amniocar nutrient medium that contains fetal calf serum (FCS and growth factors cocktail for mass cultivation of human fibroblasts. To study proliferative activity of the medium on cultures of HUVEC cells of mesenchymal origin and mesenchymal stromal cells, as well as on cell culture of human amniotic fluid.Materials and methods. Determination of the rate of accumulation of the cellular mass and cell morphology in the course of cultivation of cells of various histogenesis in the Amniocar medium and nutrient medium that contains 10 % of FCS.Results. It has been demonstrated that the Amniocar medium is prevalent as compared to the standard DMEM medium with 10 % of FCS by 2 to 5 times for cultivation of skin fibroblasts, HUVEC, and mesenchymal stem cells. The Amniocar medium increased the quantity of endothelial cells that enter mitosis and maintained the culture of HUVEC cells with prolonged passaging in vitro. Clonal cultivation of human amniotic fluid cells in the Amniocar medium secured development of colonies of both fibroblast and epithelial type.Conclusions. Proliferative Amniocar medium is efficient for mass cultivation of various cells of mesenchymal origin and can be used for diagnostic purposes in medical genetics, oncology, etc.

  8. The Study of Birefringent Homogenous Medium with Geometric Phase

    International Nuclear Information System (INIS)

    Banerjee, Dipti

    2010-12-01

    The property of linear and circular birefringence at each point of the optical medium has been evaluated here from differential matrix N using the Jones calculus. This matrix lies on the OAM sphere for l = 1 orbital angular momentum. The geometric phase is developed by twisting the medium uniformly about the direction of propagation of the light ray. The circular birefringence of the medium, is visualized through the solid angle and the angular twist per unit thickness of the medium, k, that is equivalent to the topological charge of the optical element. (author)

  9. The influence of the interstellar medium on climate and life

    International Nuclear Information System (INIS)

    Talbot, R.J. Jr.

    1980-01-01

    Recent studies of the gas and dust between the stars, the interstellar medium, reveal a complex chemistry which indicates that prebiotic organic chemistry is ubiquitous. The relationship between this interstellar chemistry and the organic chemistry of the early solar system and the Earth is explored. The interstellar medium is also considered as likely to have a continuing influence upon the climate of the Earth and other planets. Life forms as known are not only descendants of the organic evolution begun in the interstellar medium, but their continuing evolution is also molded through occasional interactions between the interstellar medium, the Sun and the climate on Earth. (author)

  10. Risk of application of contrast medium in computed tomography

    International Nuclear Information System (INIS)

    Wende, S.; Speck, U.; Schering A.G., Berlin

    1981-01-01

    The paper deals with the risk associated with the application of contrast medium in CT. The risk is to be seen in intolerance reactions, such as allergic or circulatory reactions and neurotoxic side-effects. In this paper the problems of renal failure caused by the injection of contrast medium are given special attention. Furthermore an iodine-induced hyperthyreosis might result. Especially the possible disturbance of the kidney function means that contrast medium should not be applied arbitrarily and that the examination should be done only by experienced staff. Furthermore the indication for the application of contrast medium in CT should be strictly qualified. (orig.) [de

  11. Risk of application of contrast medium in computed tomography

    Energy Technology Data Exchange (ETDEWEB)

    Wende, S.; Speck, U.

    1981-06-01

    The paper deals with the risk associated with the application of contrast medium in CT. The risk is to be seen in intolerance reactions, such as allergic or circulatory reactions and neurotoxic side-effects. In this paper the problems of renal failure caused by the injection of contrast medium are given special attention. Furthermore an iodine-induced hyperthyreosis might result. Especially the possible disturbance of the kidney function means that contrast medium should not be applied arbitrarily and that the examination should be done only by experienced staff. Furthermore the indication for the application of contrast medium in CT should be strictly qualified.

  12. Finding purchase activity patterns in small & medium enterprises

    NARCIS (Netherlands)

    Vegter, Geert J.

    2015-01-01

    Finding purchase activity patterns in Small & Medium Enterprises in a research program to enable SMEs to improve their purchase and company performance. Posterpresentatie KCO conferentie, 16 november 2015.

  13. [THE NATIONAL NUTRIENT MEDIUM FOR DIAGNOSTIC OF PURULENT BACTERIAL MENINGITIS].

    Science.gov (United States)

    Podkopaev, Ya V; Domotenko, L V; Morozova, T P; Khramov, M K; Shepelin, A P

    2015-05-01

    The national growth mediums were developed for isolating and cultivating of main agents of purulent bacterial meningitis--haemophilus agar, chocolate agar, PBM-agar. The growing and selective characteristics of developed growth mediums are examined. The haemophilus agar ensures growth of Haemophilus influenzae. The chocolate agar, PBM-agar ensure growth of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. By growing characteristics, the national growth mediums match foreign analogues. Under application of growth mediums with selective additions it is possible to achieve selective isolation of main agents of purulent bacterial meningitis with inhibition of growth of microbes-associates.

  14. Cultivation of tea fungus on malt extract medium

    Directory of Open Access Journals (Sweden)

    Cvetković Dragoljub D.

    2002-01-01

    Full Text Available The possibility of application of malt extract as a source of carbohydrate in a medium for tea fungus was investigated. The beverage obtained on such medium was compared with that prepared in a traditional way with sucrose medium. The presence of easily adoptable sugars, glucose and fructose, as dominant in malt medium results in a very effective fermentation, which gives much more sour beverage for the same time and makes it possible to reduce the fermentation period. The obtained beverage has satisfactory sensorial characteristics.

  15. A Dietary Medium-Chain Fatty Acid, Decanoic Acid, Inhibits Recruitment of Nur77 to the HSD3B2 Promoter In Vitro and Reverses Endocrine and Metabolic Abnormalities in a Rat Model of Polycystic Ovary Syndrome.

    Science.gov (United States)

    Lee, Bao Hui; Indran, Inthrani Raja; Tan, Huey Min; Li, Yu; Zhang, Zhiwei; Li, Jun; Yong, Eu-Leong

    2016-01-01

    Hyperandrogenism is the central feature of polycystic ovary syndrome (PCOS). Due to the intricate relationship between hyperandrogenism and insulin resistance in PCOS, 50%-70% of these patients also present with hyperinsulinemia. Metformin, an insulin sensitizer, has been used to reduce insulin resistance and improve fertility in women with PCOS. In previous work, we have noted that a dietary medium-chain fatty acid, decanoic acid (DA), improves glucose tolerance and lipid profile in a mouse model of diabetes. Here, we report for the first time that DA, like metformin, inhibits androgen biosynthesis in NCI-H295R steroidogenic cells by regulating the enzyme 3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomerase type 2 (HSD3B2). The inhibitory effect on HSD3B2 and androgen production required cAMP stimulation, suggesting a mechanistic action via the cAMP-stimulated pathway. Specifically, both DA and metformin reduced cAMP-enhanced recruitment of the orphan nuclear receptor Nur77 to the HSD3B2 promoter, coupled with decreased transcription and protein expression of HSD3B2. In a letrozole-induced PCOS rat model, treatment with DA or metformin reduced serum-free testosterone, lowered fasting insulin, and restored estrous cyclicity. In addition, DA treatment lowered serum total testosterone and decreased HSD3B2 protein expression in the adrenals and ovaries. We conclude that DA inhibits androgen biosynthesis via mechanisms resulting in the suppression of HSD3B2 expression, an effect consistently observed both in vitro and in vivo. The efficacy of DA in reversing the endocrine and metabolic abnormalities of the letrozole-induced PCOS rat model are promising, raising the possibility that diets including DA could be beneficial for the management of both hyperandrogenism and insulin resistance in PCOS.

  16. Optical activity via Kerr nonlinearity in a spinning chiral medium

    Energy Technology Data Exchange (ETDEWEB)

    Khan, Anwar Ali, E-mail: anwarali@uom.edu.pk [Department of Physics, University of Malakand at Chakdara Dir(L) (Pakistan); Bacha, Bakht Amin, E-mail: aminoptics@gmail.com [Department of Physics, University of Malakand at Chakdara Dir(L) (Pakistan); Khan, Rahmat Ali, E-mail: rahmat_alipk@yahoo.com [Department of Mathematics, University of Malakand (Pakistan)

    2016-11-11

    Optical activity is investigated in a chiral medium by employing the four level cascade atomic model, in which the optical responses of the atomic medium are studied with Kerr nonlinearity. Light entering into a chiral medium splits into circular birefringent beams. The angle of divergence between the circular birefringent beams and the polarization states of the two light beams is manipulated with Kerr nonlinearity. In the stationary chiral medium the angle of divergence between the circular birefringent beams is calculated to be 1.3 radian. Furthermore, circular birefringence is optically controlled in a spinning chiral medium, where the maximum rotary photon drag angle for left (right) circularly polarized beam is ±1.1 (±1.5) microradian. The change in the angle of divergence between circular birefringent beams by rotary photon drag is calculated to be 0.4 microradian. The numerical results may help to understand image designing, image coding, discovery of photonic crystals and optical sensing technology. - Highlights: • Coherent control of a circular birefringence in a chiral medium is studied. • Angle of divergence between birefringent beams is modified with Kerr nonlinearity. • Rotary photon drag is controlled for birefringent beams and enhanced with Kerr nonlinearity in a spinning medium. • Rotation of the angle of divergence is observed with mechanical rotation of the medium about an axis and modified with Kerr effect. • A change in the angle of divergence is calculated by about a microradian with rotary photon drag.

  17. Tap Water Hydraulic Systems for Medium Power Applications

    DEFF Research Database (Denmark)

    Conrad, Finn; Adelstorp, Anders

    1998-01-01

    Presentation of new range of developed tap water hydraulic componets and applications for medium power up to 4 kW and 50 bar.......Presentation of new range of developed tap water hydraulic componets and applications for medium power up to 4 kW and 50 bar....

  18. Development of a minimal growth medium for Lactobacillus plantarum

    NARCIS (Netherlands)

    Wegkamp, H.B.A.; Teusink, B.; Vos, de W.M.; Smid, E.J.

    2010-01-01

    Aim: A medium with minimal requirements for the growth of Lactobacillus plantarum WCFS was developed. The composition of the minimal medium was compared to a genome-scale metabolic model of L. plantarum. Methods and Results: By repetitive single omission experiments, two minimal media were

  19. English medium of instruction: A situation analysis | Uys | South ...

    African Journals Online (AJOL)

    The majority of learners in southern Africa receive their education through the medium of a second language, English. Although teachers of English play a crucial role in helping learners to acquire language skills in the medium of instruction, we argue that subject content teachers' lack of attention to the teaching of the four ...

  20. Medium response to jets in heavy ion collisions

    Science.gov (United States)

    Tachibana, Yasuki

    2018-01-01

    A short overview on recent progress in studies of medium response to jet quenching in heavy ion collisions is presented. We show the typical features of medium response and give comment on their connection to jet observables by introducing the work done by the author and collaborators as an example.

  1. Wave velocities in a pre-stressed anisotropic elastic medium

    Indian Academy of Sciences (India)

    Modified Christoffel equations are derived for three-dimensional wave propagation in a general anisotropic medium under initial stress.The three roots of a cubic equation define the phase velocities of three quasi-waves in the medium.Analytical expressions are used to calculate the directional derivatives of phase ...

  2. Optimization of growth medium and fermentation conditions for ...

    African Journals Online (AJOL)

    A sequential optimization approach based on statistical experimental designs was employed to optimize growth medium and fermentation conditions, in order to improve the antibiotic activity of Xenorhabdus nematophila TB. Tryptone soyptone broth (TSB) was chosen as the original medium for optimization. Glucose and ...

  3. RX emission of thin astrophysical plasma: interstellar medium and intra-cluster medium

    International Nuclear Information System (INIS)

    Arnaud, Monique

    1984-01-01

    As previous publications presented an important discrepancy of ionisation rates in astrophysical plasmas, this research thesis first reports a systematic study (by isoelectric sequence) of ionisation cross sections, based on measurements performed by mono-energetic beams, and on quantum assessments. The author proposes simple analytic fits for ionisation rates, for direct ionisation and for excitation-self-ionisation of ions of interest in astrophysics. He reports a critical review of recombination rates published in the literature, and the calculation of radiative recombination rates for different ions (hydrogen-like, helium-like, and lithium-like). Software have then been developed to determine the ionisation rate at the equilibrium and out of it for thin plasma, and to obtain ion fraction tables for different ions (H, He, C, N, O, Ne, Na, Mg, Al, Si, S, Ar, Ca, Fe, Ni). Then, a software integrating recent data on collisional excitation rates has been used to calculate the emission spectrum of a thin plasma with respect to temperature. These results are then used for the study of the interstellar medium and of supernovae remnants, and finally for the study of the intra-cluster medium [fr

  4. A comparison of medium voltage static transfer switches and medium voltage mechanical transfer switches

    Energy Technology Data Exchange (ETDEWEB)

    Risko, W. P.

    2002-07-01

    Medium voltage static transfer switches (MVSTS) and medium voltage mechanical transfer switches (MVATS) perform a common function, namely selecting between two independent power sources to provide uninterrupted power to the loads. Although the functions are the same the method of performing that function is different and this method impacts the sources and connected load. This article describes the two methods of transfer -- mechanical and static -- their advantages and disadvantages, and their preferred applications. The MVSTS can be incorporated into many applications; it can work in conjunction with backup sources such as generators; and can replace generators as a low cost solution. The reliability of the MVSTS is very high; it also outperforms the MVATS with regard to transfer speed, and can react to anomalies in the same sub-cycle time frame. Because the design of the MVSTS is modular, it can be engineered and designed to fit into existing and future systems and applications, and can be used with different switchgear variations and protection arrangements. For example, load isolation and protection breakers can be added to the switchgear to provide flexibility and isolation.

  5. Jet-induced medium excitation in heavy-ion collisions

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Wei [Key Laboratory of Quark and Lepton Physics (MOE) and Institute of Particle Physics, Central China Normal University, Wuhan 430079 (China); Pang, Long-Gang [Frankfurt Institute for Advanced Studies, Ruth-Moufang-Strasse 1, 60438 Frankfurt am Main (Germany); Stoecker, Horst [Frankfurt Institute for Advanced Studies, Ruth-Moufang-Strasse 1, 60438 Frankfurt am Main (Germany); Gesellschaft für Schwehrionenforschung, Planckstr. 1, Darmstadt (Germany); Luo, Tan; Wang, Enke [Key Laboratory of Quark and Lepton Physics (MOE) and Institute of Particle Physics, Central China Normal University, Wuhan 430079 (China); Wang, Xin-Nian [Key Laboratory of Quark and Lepton Physics (MOE) and Institute of Particle Physics, Central China Normal University, Wuhan 430079 (China); Nuclear Science Division Mailstop 70R0319, Lawrence Berkeley National Laboratory, Berkeley, CA 94740 (United States)

    2016-12-15

    We use a Linear Boltzmann Transport (LBT) model coupled to the (3+1)D ideal hydrodynamic evolution in real time with fluctuating initial conditions to simulate both the transport of jet shower partons and jet-induced medium excitation. In this coupled approach, propagation of energetic shower partons are treated in the LBT model with the 3+1D hydrodynamic model providing the evolving bulk medium. Soft partons from both elastic and inelastic processes in the LBT are fed back into the medium as a source term in the 3+1D hydrodynamics leading to induced medium excitation. We study the effect of jet-induced medium excitation via γ-hadron correlation within this coupled LBT-hydro (CoLBT-hydro) approach.

  6. Rapid mounting of adult Drosophila structures in Hoyer's medium.

    Science.gov (United States)

    Stern, David L; Sucena, Elio

    2012-01-01

    The Drosophila cuticle carries a rich array of morphological details. Thus, cuticle examination has had a central role in the history of genetics. This protocol describes a procedure for mounting adult cuticles in Hoyer's medium, a useful mountant for both larval and adult cuticles. The medium digests soft tissues rapidly, leaving the cuticle cleared for observation. In addition, samples can be transferred directly from water to Hoyer's medium. However, specimens mounted in Hoyer's medium degrade over time. For example, the fine denticles on the larval dorsum are best observed soon after mounting; they begin to fade after 1 week, and can disappear completely after several months. More robust features, such as the ventral denticle belts, will persist for a longer period of time. Because adults cannot profitably be mounted whole in Hoyer's medium, some dissection is necessary.

  7. System and method for treatment of a medium

    Science.gov (United States)

    Singh, Surinder Prabhjot; Acharya, Harish Radhakrishna; Perry, Robert James; McDermott, John Brian

    2017-05-23

    A system and method for treatment of a medium is disclosed. The system includes a plurality of separator zones and a plurality of heat transfer zones. Each of the separator zone and the heat transfer zone among the plurality of separator zones and heat transfer zones respectively, are disposed alternatively in a flow duct. Further, each separator zone includes an injector device for injecting a sorbent into the corresponding separator zone. Within the corresponding separator zone, the injected sorbent is reacted with a gaseous medium flowing in the flow duct, so as to generate a reacted gaseous medium and a reacted sorbent. Further, each heat transfer zone exchanges heat between the reacted gaseous medium fed from the corresponding separator zone and a heat transfer medium.

  8. Propagation of monochromatic light in a hot and dense medium

    Energy Technology Data Exchange (ETDEWEB)

    Masood, Samina S. [University of Houston Clear Lake, Department of Physical and Applied Sciences, Houston, TX (United States)

    2017-12-15

    Photons, as quanta of electromagnetic fields, determine the electromagnetic properties of an extremely hot and dense medium. Considering the properties of the photons in the interacting medium of charged particles, we explicitly calculate the electromagnetic properties such as the electric permittivity, magnetic permeability, refractive index and the propagation speed of electromagnetic signals in an extremely hot and dense background. Photons acquire a dynamically generated mass in such a medium. The screening mass of the photon, the Debye shielding length and the plasma frequency are calculated as functions of the statistical parameters of the medium. We study the properties of the propagating particles in astrophysical systems of distinct statistical conditions. The modifications in the properties of the medium lead to the equation of state of the system. We mainly calculate all these parameters for extremely high temperatures of the early universe. (orig.)

  9. Small and medium business in the contractual relationship system

    Directory of Open Access Journals (Sweden)

    Obalyaeva Julia, I.

    2015-01-01

    Full Text Available The paper reveals the role of small and medium business in the national economy. The comparative analysis of the indicators characterizing the development of small business in Russia, South Korea and France is given are compared. Two laws that support small and medium businesses in public purchasing. The author’s vision of the problems of development of small and medium-sized businesses in the Russian Federation and specification of the path of the national economy development is presented. Two schemes, revealing the organization of interaction of large corporate sector and the sector of small and medium-sized enterprises in the system of contractual relations and analysis of internal and external factors in the development of small and medium-sized enterprises are presented. The experience of conducting digital auctions with participation of small businesses on an digital platform CJSC "Sberbank AST" is considered.

  10. Propagation of monochromatic light in a hot and dense medium

    Science.gov (United States)

    Masood, Samina S.

    2017-12-01

    Photons, as quanta of electromagnetic fields, determine the electromagnetic properties of an extremely hot and dense medium. Considering the properties of the photons in the interacting medium of charged particles, we explicitly calculate the electromagnetic properties such as the electric permittivity, magnetic permeability, refractive index and the propagation speed of electromagnetic signals in an extremely hot and dense background. Photons acquire a dynamically generated mass in such a medium. The screening mass of the photon, the Debye shielding length and the plasma frequency are calculated as functions of the statistical parameters of the medium. We study the properties of the propagating particles in astrophysical systems of distinct statistical conditions. The modifications in the properties of the medium lead to the equation of state of the system. We mainly calculate all these parameters for extremely high temperatures of the early universe.

  11. Isogeometric frictionless contact analysis with the third medium method

    Science.gov (United States)

    Kruse, R.; Nguyen-Thanh, N.; Wriggers, P.; De Lorenzis, L.

    2018-01-01

    This paper presents an isogeometric formulation for frictionless contact between deformable bodies, based on the recently proposed concept of the third medium. This concept relies on continuum formulations not only for the contacting bodies but also for a fictitious intermediate medium in which the bodies can move and interact. Key to the formulation is a suitable definition of the constitutive behavior of the third medium. In this work, based on a number of numerical tests, the role of the material parameters of the third medium is systematically assessed. We also assess the rate of spatial convergence for higher-order discretizations, stemming from the regularization of the non-smooth contact problem inherent to the third medium approach. Finally, problems with self contact are considered and turn out to be an attractive application of the method.

  12. Bacterial growth on Mueller Hinton medium sterilized by. gamma. -radiation

    Energy Technology Data Exchange (ETDEWEB)

    Eisenberg, E. (Israel Atomic Energy Commission, Yavne. Soreq Nuclear Research Center); Bogokowsky, B.; Altmann, G. (Sheba Medical Center, Tel Hashomer (Israel); Tel Aviv Univ. (Israel). Medical School)

    1981-12-01

    The possibility of radiosterilization of culture media for the cultivation of bacteria was investigated. Mueller Hinton agar, a medium widely used for the propagation of some fastidious pathogenic bacteria, was sterilized with 1.0 and 1.5 Mrad doses of ..gamma..-radiation. Bacteria belonging to seven different species grew as well on the radiosterilized medium as on medium sterilized by heat in a conventional way. Reduction in colony size was observed on the radiosterilized medium with Staphylococcus aureus and Shigella boydii. Neisseria meningitidis, the most fastidious bacterium tested, did not grow at all. The addition of small amounts of catalase corrected the deleterious radiation effect and all bacteria tested could be successfully grown on irradiated Mueller Hinton agar supplemented with up to 11 Keil units catalase per liter medium.

  13. van der Waals interactions in a magnetodielectric medium

    International Nuclear Information System (INIS)

    Spagnolo, S.; Dalvit, D. A. R.; Milonni, P. W.

    2007-01-01

    The van der Waals interaction between two ground-state atoms is calculated for two electrically or magnetically polarizable particles embedded in a dispersive magnetodielectric medium. Unlike previous calculations which infer the atom-atom interaction from the dilute-medium limit of the macroscopic, many-body van der Waals interaction, the interaction is calculated directly for the system of two atoms in a magnetodielectric medium. Two approaches are presented, the first based on the quantized electromagnetic field in a dispersive medium without absorption and the second on Green functions that allow for absorption. We show that the correct van der Waals interactions are obtained regardless of whether absorption in the host medium is explicitly taken into account

  14. Medium properties and total energy coupling in underground explosions

    International Nuclear Information System (INIS)

    Kurtz, S.R.

    1975-01-01

    A phenomenological model is presented that allows the direct calculation of the effects of variations in medium properties on the total energy coupling between the medium and an underground explosion. The model presented is based upon the assumption that the shock wave generated in the medium can be described as a spherical blast wave at early times. The total energy coupled to the medium is then simply the sum of the kinetic and internal energies of this blast wave. Results obtained by use of this model indicate that the energy coupling is more strongly affected by the medium's porosity than by its water content. These results agree well with those obtained by summing the energy deposited by the blast wave as a function of range

  15. The Quiet Rise of Medium-Scale Farms in Malawi

    Directory of Open Access Journals (Sweden)

    Ward Anseeuw

    2016-06-01

    Full Text Available Medium-scale farms have become a major force in Malawi’s agricultural sector. Malawi’s most recent official agricultural survey indicates that these account for over a quarter of all land under cultivation in Malawi. This study explores the causes and multifaceted consequences of the rising importance of medium-scale farms in Malawi. We identify the characteristics and pathways of entry into farming based on surveys of 300 medium-scale farmers undertaken in 2014 in the districts of Mchinji, Kasungu and Lilongwe. The area of land acquired by medium-scale farmers in these three districts is found to have almost doubled between 2000 and 2015. Just over half of the medium-scale farmers represent cases of successful expansion out of small-scale farming status; the other significant proportion of medium-scale farmers are found to be urban-based professionals, entrepreneurs and/or civil servants who acquired land, some very recently, and started farming in mid-life. We also find that a significant portion of the land acquired by medium-scale farmers was utilized by others prior to acquisition, that most of the acquired land was under customary tenure, and that the current owners were often successful in transferring the ownership structure of the acquired land to a long-term leaseholding with a title deed. The study finds that, instead of just strong endogenous growth of small-scale famers as a route for the emergence of medium-scale farms, significant farm consolidation is occurring through land acquisitions, often by urban-based people. The effects of farmland acquisitions by domestic investors on the country’s primary development goals, such as food security, poverty reduction and employment, are not yet clear, though some trends appear to be emerging. We consider future research questions that may more fully shed light on the implications of policies that would continue to promote land acquisitions by medium-scale farms.

  16. In-medium meson properties and screening correlators

    International Nuclear Information System (INIS)

    Bazavov, A; Karsch, F; Mukherjee, Swagato; Petreczky, P; Maezawa, Y

    2014-01-01

    We study spatial meson correlation functions consisting of strange quarks, strange and charm quarks and charm quarks in (2 + 1)-flavor QCD using the highly improved staggered quark action. We find that the in-medium modification of the meson correlators decreases with increasing charm quark content and decreasing size. In particular, we find strong in-medium modification of φ and D s meson correlators around the chiral transition temperature T c , while J/ψ and η c correlators show strong in-medium modification only at temperatures of 1.4T c .

  17. Analytic descriptions of cylindrical electromagnetic waves in a nonlinear medium

    Science.gov (United States)

    Xiong, Hao; Si, Liu-Gang; Yang, Xiaoxue; Wu, Ying

    2015-01-01

    A simple but highly efficient approach for dealing with the problem of cylindrical electromagnetic waves propagation in a nonlinear medium is proposed based on an exact solution proposed recently. We derive an analytical explicit formula, which exhibiting rich interesting nonlinear effects, to describe the propagation of any amount of cylindrical electromagnetic waves in a nonlinear medium. The results obtained by using the present method are accurately concordant with the results of using traditional coupled-wave equations. As an example of application, we discuss how a third wave affects the sum- and difference-frequency generation of two waves propagation in the nonlinear medium. PMID:26073066

  18. Steering of Educational Processes in a Digital Medium Environment

    DEFF Research Database (Denmark)

    Tække, Jesper; Paulsen, Michael

    2015-01-01

    by systems theory we outline a more adequate way of teaching in the new medium environment – a teaching that can manage the new situation and use the new possibilities provided by the digital media. The argumentation builds on empirical findings from the action research project Socio Media Education (SME......This paper is about challenges to steering and leadership of educational interaction in classrooms provided by the new medium environment that comes with digital media. In the new medium environment, the old way of steering what is going on in the classroom appears not to work since...

  19. Organic chemistry and biology of the interstellar medium

    Science.gov (United States)

    Sagan, C.

    1973-01-01

    Interstellar organic chemistry is discussed as the field of study emerging from the discovery of microwave lines of formaldehyde and of hydrogen cyanide in the interstellar medium. The reliability of molecular identifications and comparisons of interstellar and cometary compounds are considered, along with the degradational origin of simple organics. It is pointed out that the contribution of interstellar organic chemistry to problems in biology is not substantive but analogical. The interstellar medium reveals the operation of chemical processes which, on earth and perhaps on vast numbers of planets throughout the universe, led to the origin of life, but the actual molecules of the interstellar medium are unlikely to play any significant biological role.

  20. Collective effects in shock propagation through a clumpy medium

    International Nuclear Information System (INIS)

    Norman, M.L.; Dickel, J.R.; Livio, M.; Chu, Y.H.

    1988-01-01

    A numerical simulation of shock propagation in a clumpy medium with a weak magnetic field is presented which illustrates a number of dynamical processes of potential importance for explaining spectral line width and radio polarization measurements in supernova remnants

  1. Health and safety management practices in small and medium ...

    African Journals Online (AJOL)

    Health and safety management practices in small and medium enterprises in the ... on national economies, the construction industry receives additional attention in ... and training were considered key factors of H&S for SMCEs at project level.

  2. On Emulation of Flueric Devices in Excitable Chemical Medium.

    Directory of Open Access Journals (Sweden)

    Andrew Adamatzky

    Full Text Available Flueric devices are fluidic devices without moving parts. Fluidic devices use fluid as a medium for information transfer and computation. A Belousov-Zhabotinsky (BZ medium is a thin-layer spatially extended excitable chemical medium which exhibits travelling excitation wave-fronts. The excitation wave-fronts transfer information. Flueric devices compute via jets interaction. BZ devices compute via excitation wave-fronts interaction. In numerical model of BZ medium we show that functions of key flueric devices are implemented in the excitable chemical system: signal generator, and, xor, not and nor Boolean gates, delay elements, diodes and sensors. Flueric devices have been widely used in industry since late 1960s and are still employed in automotive and aircraft technologies. Implementation of analog of the flueric devices in the excitable chemical systems opens doors to further applications of excitation wave-based unconventional computing in soft robotics, embedded organic electronics and living technologies.

  3. Bound values for Hall conductivity of heterogeneous medium under ...

    Indian Academy of Sciences (India)

    - ditions in inhomogeneous medium has been studied. It is shown that bound values for. Hall conductivity differ from bound values for metallic conductivity. This is due to the unusual character of current percolation under quantum Hall effect ...

  4. Culture medium for amylase production by toxigenic fungi

    Directory of Open Access Journals (Sweden)

    Figueira Edson Luiz Zangrando

    2000-01-01

    Full Text Available Mycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p<0.05, and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged promoted the highest amylase production, which was at the 10th day of fermentation, both for F. moniliforme (42.32U/mL and A. flavus (4,745.54U/mL.

  5. Response of orthotropic micropolar elastic medium due to time ...

    Indian Academy of Sciences (India)

    R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

    namic response of anisotropic continuum has received the attention of ... linear theory of micropolar elasticity and bending of orthotropic micropolar ... medium due to time harmonic concentrated load, the continuum is divided into two half-.

  6. Effects of Tempo and Performing Medium on Children's Music Preference.

    Science.gov (United States)

    LeBlanc, Albert; Cote, Richard

    1983-01-01

    This study measured the effect of three levels of tempo and two levels of performing medium, vocal and instrumental, on the expressed preference of fifth- and sixth-grade students for traditional jazz music listening examples. (Author/SR)

  7. Dense Medium Machine Processing Method for Palm Kernel/ Shell ...

    African Journals Online (AJOL)

    ADOWIE PERE

    Cracked palm kernel is a mixture of kernels, broken shells, dusts and other impurities. In ... machine processing method using dense medium, a separator, a shell collector and a kernel .... efficiency, ease of maintenance and uniformity of.

  8. Optimization of critical medium components for higher phycocyanin ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-09-01

    Sep 1, 2009 ... culture medium was screened and optimized using the statistical experimental designs of Plackett- .... statistical inference for exploring the functional relation- ..... from the F-test with a very low probability value (0.0015).

  9. Options for Sustaining Small and Medium Scale Enterprises in ...

    African Journals Online (AJOL)

    FIRST LADY

    problems in Nigeria due to numerous domestic and global economic problems, and policy ... national income till today remains low (Osamwonyi, 2009). For ... managers. Nwakoby (1988) defines Small and Medium-Scale business as “any.

  10. Modification of Einstein A Coefficient in Dissipative Gas Medium

    Science.gov (United States)

    Cao, Chang-Qi; Cao, Hui; Qin, Ke-Cheng

    1996-01-01

    Spontaneous radiation in dissipative gas medium such as plasmas is investigated by Langevin equations and the modified Weisskopf-Wigner approximation. Since the refractive index of gas medium is expected to be nearly unity, we shall first neglect the medium polarization effect. We show that absorption in plasmas may in certain case modify the Einstein A coefficient significantly and cause a pit in the A coefficient-density curves for relatively low temperature plasmas and also a pit in the A coefficient-temperature curves. In the next, the effect of medium polarization is taken into account in addition. To our surprise, its effect in certain case is quite significant. The dispersive curves show different behaviors in different region of parameters.

  11. Inmates perception of the living conditions in a medium security ...

    African Journals Online (AJOL)

    Inmates perception of the living conditions in a medium security prison in North ... and adopted a number of International legal instruments to protect and guarantee ... Data analysis was done with Statistical Package for Social Sciences version ...

  12. A direct sampling method to an inverse medium scattering problem

    KAUST Repository

    Ito, Kazufumi; Jin, Bangti; Zou, Jun

    2012-01-01

    In this work we present a novel sampling method for time harmonic inverse medium scattering problems. It provides a simple tool to directly estimate the shape of the unknown scatterers (inhomogeneous media), and it is applicable even when

  13. A two-stage method for inverse medium scattering

    KAUST Repository

    Ito, Kazufumi; Jin, Bangti; Zou, Jun

    2013-01-01

    We present a novel numerical method to the time-harmonic inverse medium scattering problem of recovering the refractive index from noisy near-field scattered data. The approach consists of two stages, one pruning step of detecting the scatterer

  14. Fem Formulation of Heat Transfer in Cylindrical Porous Medium

    Science.gov (United States)

    Azeem; Khaleed, H. M. T.; Soudagar, Manzoor Elahi M.

    2017-08-01

    Heat transfer in porous medium can be derived from the fundamental laws of flow in porous region ass given by Henry Darcy. The fluid flow and energy transport inside the porous medium can be described with the help of momentum and energy equations. The heat transfer in cylindrical porous medium differs from its counterpart in radial and axial coordinates. The present work is focused to discuss the finite element formulation of heat transfer in cylindrical porous medium. The basic partial differential equations are derived using Darcy law which is the converted into a set of algebraic equations with the help of finite element method. The resulting equations are solved by matrix method for two solution variables involved in the coupled equations.

  15. Medium-Term Results After Treatment of Recalcitrant Lateral Epicondylitis

    Science.gov (United States)

    Meknas, Khaled; Al Hassoni, Thabit N.; Odden-Miland, Åshild; Castillejo, Miguel; Kartus, Jüri

    2013-01-01

    Background: Recalcitrant lateral epicondylitis (elbow extensor–origin tendinosis) is a common cause of elbow pain with many treatment options. In the present study, the medium-term results after open release and radiofrequency microtenotomy are reported. Hypothesis: Microtenotomy would provide long-term pain relief that was as good as the open release method. Study Design: Prospective, randomized trial. Methods: Twenty-four patients randomized to either open release or microtenotomy were assessed after 5 to 7 years. Clinical examination and dynamic infrared thermography (DIRT) of both elbows were performed preoperatively and at the medium-term follow-up. Magnetic resonance imaging (MRI) of both elbows was performed at the medium-term follow-up. Results: Significant pain reduction was found using a visual analog scale (VAS) at the medium-term follow-up in both groups compared with the preoperative assessment (P lateral epicondylitis. The hypothesis was thus verified. PMID:26535247

  16. Development of a new medium containing date syrup for production ...

    African Journals Online (AJOL)

    USER

    2010-08-16

    Aug 16, 2010 ... A confirmatory experiment of the optimal medium composition ... product of date industry; it is rich in carbohydrates (75% w/w) and small ... producers at cheap prices. ... date syrup using a combination of statistical strategies.

  17. Electromagnetic radiation of ultrarelativistic particles at scattering in excited medium

    International Nuclear Information System (INIS)

    Malyshevskij, V.S.

    1990-01-01

    The interaction between relativistic particles and a gaseous or condensed medium with a high density of nondegenerate excited quantum states involves the coherent conversion of atomic or molecular excitations into electromagnetic radiation

  18. Medium Range Forecast (MRF) and Nested Grid Model (NGM)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Nested Grid Model (NGM) and Medium Range Forecast (MRF) Archive is historical digital data set DSI-6140, archived at the NOAA National Centers for Environmental...

  19. Defining Small and Medium Enterprises: a critical review

    Directory of Open Access Journals (Sweden)

    Gentrit Berisha

    2015-03-01

    Full Text Available The OECD estimates that small and medium enterprises account for 90% of firms and employ 63% of the workforce in the world (Munro: 2013. Small and medium enterprises account for that amount of businesses thatit is senseless the arbitrariness with which they are defined. Language mainly used for definition is numbers, but it is difficult to find two institutions, statistical agencies or countries who speak the same language in terms of small and medium enterprises. Academics, authors, policy makers apply SMEdefinitions in terms of dichotomy between universality and standardization of a unique definition and relativity and sectored specialization. Although qualitative criteria-characteristics of SMEs easily distinguish them from large businesses, quantitative criteria are mainlyused for their dimensional classification. This paper deals with a critical approachto the definition of small and medium enterprises, inconsistencies in criteria and various proposed approaches to the definition towards universal acceptance.

  20. Medium resolution image fusion, does it enhance forest structure assessment

    CSIR Research Space (South Africa)

    Roberts, JW

    2008-07-01

    Full Text Available This research explored the potential benefits of fusing optical and Synthetic Aperture Radar (SAR) medium resolution satellite-borne sensor data for forest structural assessment. Image fusion was applied as a means of retaining disparate data...

  1. On Emulation of Flueric Devices in Excitable Chemical Medium.

    Science.gov (United States)

    Adamatzky, Andrew

    2016-01-01

    Flueric devices are fluidic devices without moving parts. Fluidic devices use fluid as a medium for information transfer and computation. A Belousov-Zhabotinsky (BZ) medium is a thin-layer spatially extended excitable chemical medium which exhibits travelling excitation wave-fronts. The excitation wave-fronts transfer information. Flueric devices compute via jets interaction. BZ devices compute via excitation wave-fronts interaction. In numerical model of BZ medium we show that functions of key flueric devices are implemented in the excitable chemical system: signal generator, and, xor, not and nor Boolean gates, delay elements, diodes and sensors. Flueric devices have been widely used in industry since late 1960s and are still employed in automotive and aircraft technologies. Implementation of analog of the flueric devices in the excitable chemical systems opens doors to further applications of excitation wave-based unconventional computing in soft robotics, embedded organic electronics and living technologies.

  2. DIORAMA ART – A POTENTIAL MEDIUM FOR MUSEUM ...

    African Journals Online (AJOL)

    User

    The study recognizes the traditional role diorama plays as an effective medium for museum edu- cation and employed both ... Keywords: Diorama, Museum, Education, Aperture, Assemblage. ... velopment of the modern world. Aside the open ...

  3. Export Marketing Involvement of African Small and Medium Size ...

    African Journals Online (AJOL)

    Export Marketing Involvement of African Small and Medium Size Enterprises: Tanzania's Fish Processing Industry. ... This study examined the Export marketing involvement of SMEs from the Least ... EMAIL FULL TEXT EMAIL FULL TEXT

  4. Kinetics of molybdenite oxidizing leaching in alkali medium by ozone

    International Nuclear Information System (INIS)

    Medvedev, A.S.; Sokratova, N.B.; Litman, I.V.; Zelikman, A.N.

    1985-01-01

    On the basis of investigation of the process kinetics proposed is a model of oxidizing leaching of molybdenite in alkali medium while ozonization of the solution by ozoneair mixture. A kinetic equation is derived, that describes experimental data satisfactorily

  5. Theoretical Investigation of Trust in Small and Medium Sized Enterprises

    Directory of Open Access Journals (Sweden)

    Valentinas Navickas

    2014-06-01

    Full Text Available The hectic pace of competition pushes the sector of small and medium enterprise to adopt sophisticated marketing ideas. In this context, customers are becoming more and more selective. However, expansion possibilities are burdened by the great variety of limitations. The current study paper aims to investigate the academic perception of trust which is treated as competitive advantage for small and medium enterprises. The object of the article is trust in small and medium enterprises. It has three objectives: to analyse small and medium enterprise characteristics through the academic perspective; to consider trust from the theoretical point of view; and to present the models that centre their attention on the trust as a construct that enhances customer satisfaction. Regarding the novelty of the current study, it brings a novel approach on a great variety of collected understandings of trust and puts an important foundation for future theory and practice investigations.

  6. Computers in the Classroom: From Tool to Medium.

    Science.gov (United States)

    Perrone, Corrina; Repenning, Alexander; Spencer, Sarah; Ambach, James

    1996-01-01

    Discusses the computer as a communication medium to support learning. Illustrates the benefits of this reconceptualization in the context of having students author and play interactive simulation games and exchange them over the Internet. (RS)

  7. Apps: a new medium for non-fiction innovation

    OpenAIRE

    Allen, Catherine

    2015-01-01

    Apps are now a dominant content medium: in the US people spend more time on apps than they do watching TV. Non-fiction content is being avidly consumed on mobile devices, but in a completely different way to the book model. This article explores three strands of potential that the app medium holds for non-fiction content, putting forward the case that apps have the power to further weave non-fiction into the fabric of society and life.

  8. Information Security in Small and Medium-Sized Companies

    OpenAIRE

    David Kral

    2011-01-01

    Information security doesn’t involve only large organizations. Small and medium-sized companies must closely examine this issue too, because they are increasingly threatened by cyber attacks. Many of them mistakenly believe, that security of their valuable data is sufficient, or that the attackers are not interested in them. Existing standards and methodologies for implementation and management of information security are often hard to transfer to the environment of small and medium-sized bus...

  9. ORGANIZATIONAL CHANGE MODELS IN SMALL AND MEDIUM SIZED ENTERPRISES (SMES)

    OpenAIRE

    Oliviana Bold, Ph. D Student

    2011-01-01

    Bringing forward the concepts of change and change management is no longer surprising nowadays. Small and Medium Enterprises (SMEs) face unique and difficult challenges in the business environment. Challenges to the growth and viability of Small and Medium Enterprises (SMEs) are arising from several external factors, like globalization, increased customer expectations or competition, technological advances, all of the factors being determined by the change. SMEs need to successfully deal with...

  10. Franchise Business Sustainability – West Java Province Small Medium Enterprises

    OpenAIRE

    Danil, Lilian; Septina, Nina

    2015-01-01

    Entrepreneurship has become a prime stimulant in the economic world, especially in the America, Japan, and Singapore. However, this has not yet occurred in Indonesia, supported by the data from of the Ministry of Cooperatives and Small and Medium Enterprises which mentioned about the number of Indonesian entrepreneurs which only 1.9 percent of 250 million inhabitants in 2013. Franchise business which is growing in Indonesia has a great opportunity for small and medium enterprises business dev...

  11. Hydrodynamic instability of compressible fluid in porous medium

    International Nuclear Information System (INIS)

    Argal, Shraddha; Tiwari, Anita; Sharma, P K; Prajapati, R P

    2014-01-01

    The hydrodynamic Rayleigh -Taylor instability of two superposed compressible fluids in porous medium has been studied. The dispersion relation is derived for such a medium by using normal mode analysis. The RT instability is discussed for various simplified configuration. The effect of porosity and dynamic viscosity has been analyzed and it is observed that porosity and dynamic viscosity have stabilizing effect on the Rayleigh- Taylor instability of compressible fluids.

  12. Solitons of an envelope in an inhomogeneous medium

    International Nuclear Information System (INIS)

    Churilov, S.M.

    1982-01-01

    Solutions of the Schroedinger nonlinear equation (SNE) used for the description of evolution of a wave packet envelope has been investigated in inhomogeneous and nonstationary media. It is shown that the SNE solution possessing two important properties exists. Firstly, the wave packet remains localized when propagating in an inhomogeneous medium. Secondly, the soliton width and amplitude are determined only with local characteristics of medium and don't depend on the prehistory. Problem of limits of obtained result applicability has been considered

  13. Interstellar medium structure and content and gamma ray astronomy

    International Nuclear Information System (INIS)

    Lebrun, F.

    1982-05-01

    A general description of gamma-ray astronomy is presented with special emphasis on the study of diffuse gamma-ray emission. This is followed by a collection of reflections and observations on the structure and the gas and dust content of the local interstellar medium. Results of gamma-ray observations on the local interstellar medium are given. The last part is devoted to the whole of the galactic gamma-ray emission and its interpretation [fr

  14. AN INVESTIGATION INTO MEDIUM-SIZED MULTINATIONAL ENTERPRISES

    Directory of Open Access Journals (Sweden)

    Daniele Schilirò

    2013-09-01

    Full Text Available The paper provides an investigation of medium-sized Italian industrial enterprises that have become multinational companies. It concetrates on the set of medium and medium-large enterprises who seem to grow more in foreign markets, either through exports or through foreign direct investment. The work also offers a descriptive empirical picture of the performance of medium-sized Italian multinationals, which is compared with the performance of large corporations. From this analysis, which is based on several data sources, it is possible to outline a profile regarding the medium-size italian multinational enterprises; the aim is to understand the complex strategy towards internationalization of these companies, where the dimension of production is important and, therefore, innovation has a key role. Also the commercial dimension is crucial, because it leads to point to the direct supervision of foreign markets and to look very carefully at the customers, offering them a wide range of services. Finally, the paper highlights some critical issues that the medium sized multinational enterprises have to face for competing: namely, the stagnant productivity, the high taxation, the insufficient institutional support for internationalization, the bureaucracy and its high costs, the lack of skilled human capital available in the labor market due to inadequate policy training.

  15. [A nutrient medium for the isolation and cultivation of Campylobacter].

    Science.gov (United States)

    Temirkhanova, Z U; Gashimova, P Sh; Safonova, N V; Moroz, A F; Khazenson, L V

    1999-01-01

    Campylobacter agar, nutrient medium intended for the isolation of bacteria of the genus Campylobacter from clinical material, has been developed. The composition of the medium includes sprat hydrolysate, aerotolerant additive (ferric sulfate--oxide, sodium pyruvate, sodium pyrosulfite), sodium glutaminate, agar. The selective properties of the medium are ensured by introducing the mixture of antibiotics consisting of polymyxin B, rifampicin, amphotericin B, ristomycin. The balanced composition of Campylobacter agar ensures the aerotolerance of Campylobacter organisms and gives the optimal conditions for their growth when the inoculated material is cultivated in the atmosphere made up of the mixture of three gases (5% of oxygen, 10% of carbon dioxide, 85% of nitrogen), as well as under the conditions of a "candle vessel". The medium suppresses the development of the associative microflora diluted 10(-1). As shown in the trial of the quality of Campylobacter agar by the inoculation of material taken from patients with acute enteric infections, agricultural animals and monkeys, the medium has pronounced selective, properties with regard to extraneous microflora, while ensuring the isolation of Campylobacter on the level of the control medium.

  16. Optical imaging of objects in turbid medium with ultrashort pulses

    Science.gov (United States)

    Wang, Chih-Yu; Sun, Chia-Wei; Yang, Chih Chung; Kiang, Yean-Woei; Lin, Chii-Wann

    2000-07-01

    Photons are seriously scattered when entering turbid medium; this the images of objects hidden in turbid medium can not be obtained by just collecting the transmitted photons. Early-arriving photons, which are also called ballistic or snake protons, are much less scattered when passing through turbid medium, and contains more image information than the late-arriving ones. Therefore, objects embedded in turbid medium can be imaged by gathering the ballistic and snake photons. In the present research we try to recover images of objects in turbid medium by simultaneously time-gate and polarization-gate to obtain the snake photons. An Argon-pumped Ti-Sapphire laser with 100fs pulses was employed as a light source. A streak camera with a 2ps temporal resolution was used to extract the ballistic and snake photons. Two pieces of lean swine meat, measured 4mmX3mm and 5xxX4mm, respectively, were placed in a 10cmX10cmX3cm acrylic tank, which was full of diluted milk. A pair of polarizer and an analyzer was used to extract the light that keeps polarization unchanged. The combination of time gating and polarization gating resulted in good images of objects hidden in turbid medium.

  17. Medium Affect Desire: Hybridising Real Virtual and the Actualised through Affective Medium Ecology

    Directory of Open Access Journals (Sweden)

    Marc Boumeester

    2014-10-01

    Full Text Available Underneath the turbulent surface of the ubiquitous media-scape lies an even more agile and aggressive set of relations. A central figure in this turmoil of desires seems to be the asignifying sign, which has a hybridising liaison with both the realm of the real virtual and the realm of the actualised. The main question is what does it want? This new materialistic, non-anthropocentric liberty of affect is creating an arena of strange attractors and other topological vector fields in which our own unconscious drive is as effective as that of the steel ball in a pinball machine. Could we isolate the intrinsic drive of the medium from its subservient position in the aesthetic, freeing its desire from the anthropocentric dominion? What does it Yen for? Perhaps this gap is not meant to be filled, as it is this yearning what it yearns for. The asignifying sign cannot be isolated, it is neither here nor there, yet it is conditionally omnipresent, it inhibits the gap, its desire is to affect.

  18. Choice of Scottish Gaelic-Medium and Welsh-Medium Education at the Primary and Secondary School Stages: Parent and Pupil Perspectives

    Science.gov (United States)

    O'Hanlon, Fiona

    2015-01-01

    Results are presented of a comparative study of the reasons for parental choice of Scottish Gaelic-medium and Welsh-medium primary education in the year 2000 and of the reasons for pupils' decisions to continue with Gaelic or Welsh-medium education at secondary school in 2007. Parents in both contexts cited the quality of Celtic-medium education…

  19. Medium-range dielectric order in systems with collectivized electrons

    International Nuclear Information System (INIS)

    Ismagilov, A.M.; Kopaev, Yu.V.

    1993-01-01

    The problem of formation of a medium-range dielectric order (on a scale much larger than the interatomic one) due to electron-electron correlations and to scattering by an impurity in a system near a phase transition into a long-range order state is solved by a microscopic approach. It is shown that for a weak impurity potential the effect of medium-range order formation is stronger than the effect of long-range order suppression related to scattering by an impurity. The influence of medium-range order on the one-particle excitation spectrum and on the density of states is considered. It is found that since the medium-range order in a system is due to correlations of electron and hole states open-quotes coupledclose quotes by a continuous set of inhomogeneity vectors (in contrast to the long-range order formed on a discrete set of such vectors), the density of states varies on an energy scale determined by the mean absolute value of these vectors. Therefore in a system undergoing phase transition into an inhomogeneous state with the modulus q 0 of inhomogeneity vectors the medium-range order forms in the density of states a pseudogap of scale length v F q 0 (v F is the Fermi velocity). This distinguishes such a system substantially from one, which tends to a phase transition into a homogeneous state (q 0 ≡0), where the medium-range order forms a pseudogap of scale length v F /ξ much-lt v F q 0 (ξ is the correlation length). The possible role of medium dielectric order effects in high-T c superconductors is discussed. 30 refs., 6 figs

  20. Effective-Medium Models for Marine Gas Hydrates, Mallik Revisited

    Science.gov (United States)

    Terry, D. A.; Knapp, C. C.; Knapp, J. H.

    2011-12-01

    Hertz-Mindlin type effective-medium dry-rock elastic models have been commonly used for more than three decades in rock physics analysis, and recently have been applied to assessment of marine gas hydrate resources. Comparisons of several effective-medium models with derivative well-log data from the Mackenzie River Valley, Northwest Territories, Canada (i.e. Mallik 2L-38 and 5L-38) were made several years ago as part of a marine gas hydrate joint industry project in the Gulf of Mexico. The matrix/grain supporting model (one of the five models compared) was clearly a better representation of the Mallik data than the other four models (2 cemented sand models; a pore-filling model; and an inclusion model). Even though the matrix/grain supporting model was clearly better, reservations were noted that the compressional velocity of the model was higher than the compressional velocity measured via the sonic logs, and that the shear velocities showed an even greater discrepancy. Over more than thirty years, variations of Hertz-Mindlin type effective medium models have evolved for unconsolidated sediments and here, we briefly review their development. In the past few years, the perfectly smooth grain version of the Hertz-Mindlin type effective-medium model has been favored over the infinitely rough grain version compared in the Gulf of Mexico study. We revisit the data from the Mallik wells to review assertions that effective-medium models with perfectly smooth grains are a better predictor than models with infinitely rough grains. We briefly review three Hertz-Mindlin type effective-medium models, and standardize nomenclature and notation. To calibrate the extended effective-medium model in gas hydrates, we use a well accepted framework for unconsolidated sediments through Hashin-Shtrikman bounds. We implement the previously discussed effective-medium models for saturated sediments with gas hydrates and compute theoretical curves of seismic velocities versus gas hydrate

  1. ANALYSIS OF ROMANIAN SMALL AND MEDIUM ENTERPRISES BANKRUPTCY RISK

    Directory of Open Access Journals (Sweden)

    Kulcsar Edina

    2014-07-01

    Full Text Available Considering the fundamental role of small and medium enterprises in Romanian economy, this paper aims to quantify the level of their bankruptcy risk for 2009 and 2012 period, after debuting of financial crisis. The main reason of selecting this type of companies is that they represent the backbone of national economy. They have an indispensable role, because they offer jobs for great part of population and their contribution for GDP stimulation is considerable. In this paper it was applied two default risk models, namely the well known Altman’s Z-score model, based on five financial ratios and a bankruptcy predictor model developed by Teti et. al (2012 used firstly exclusively for Italian small and medium-sized enterprise for 2006-2009 period. The model proposed by Teti et. is based on the investigation of financially distressed and financially non-distressed Italian small and medium-sized enterprises during the financial crisis by using a discriminant analysis model. They conclude that there are four financial ratios, which characterized well the small and medium-sized enterprises bankruptcy risk. These variables are financial ratios, like: Debt/Total Assets, Return on Sales (ROS, EBIT/Interest Expenses and Working capital/EBIDTA. They consider that small and medium-sized enterprises require a particular approach in terms of bankruptcy risk analysis. In present study I try to compare the efficiency of traditional bankruptcy risk model with a small and medium-sized specific model. The necessary database for present analysis is ensured by simplified financial reports of 120 small and medium-sized enterprises registered in Bihor County. The selected enterprises are operating in manufacturing industry (21,67% and trading (78,33%. Present investigation has an important value in actual economic background, where the healthiness and sustainability of small and medium-sized enterprises is a great issue. The results of study shows contradictory

  2. Oscillating electromagnetic soliton in an anisotropic ferromagnetic medium

    Energy Technology Data Exchange (ETDEWEB)

    Sathishkumar, P., E-mail: perumal_sathish@yahoo.co.in [Department of Physics, K.S.R. College of Engineering (Autonomous), Tiruchengode 637215, Tamilnadu (India); Senjudarvannan, R. [Department of Physics, Jansons Institute of Technology, Karumathampatty, Coimbatore 641659 (India)

    2017-05-01

    We investigate theoretically the propagation of electromagnetic oscillating soliton in the form of breather in an anisotropic ferromagnetic medium. The interaction of magnetization with the magnetic field component of the electromagnetic (EM) wave has been studied by solving Maxwell's equations coupled with a Landau–Lifshitz equation for the magnetization of the medium. We made a small perturbation on the magnetization and magnetic field along the direction of propagation of EM wave in the framework of reductive perturbation method and the associated nonlinear magnetization dynamics is governed by a generalized derivative nonlinear Schrödinger (DNLS) equation. In order to understand the dynamics of the concerned system, we employ the Jacobi elliptic function method to solve the DNLS equation and deduce breatherlike soliton modes for the EM wave in the medium. - Highlights: • The propagation of electromagnetic oscillating soliton in an anisotropic ferromagnetic medium is investigated in the presence of varying external magnetic field. • The magnetization and electromagnetic wave modulates in the form of breathing like oscillating solitons. • The governing nonlinear spin dynamical equation is studied through a reductive perturbation method. • The magnetization components of the ferromagnetic medium are derived using Jacobi elliptic functions method with the aid of symbolic computation.

  3. Development of a vinasse culture medium for plant tissue culture

    International Nuclear Information System (INIS)

    Silva, A.L.L.D.; Gollo, L.

    2014-01-01

    Vinasse is the main pollutant (effluent) obtained from the distillation of sugarcane in the production of fuel alcohol. However, this residue is rich in nutrients that are required by plants. We developed a new culture medium using vinasse for the In vitro propagation of an orchid. The vinasse was treated (decanted and filtered), and the nutrients were determined and quantified. Different formulations using vinasse were tested for an In vitro culture. The vinasse dilutions demonstrated a good buffering effect. The ideal vinasse dilution for media formulation was 2.5%. The best KC formulations with vinasse were KCV1 and KCV5. Compared to KC medium, these formulations demonstrated similar results for In vitro multiplication, with the exception of protocorm-like body number, which was inferior in the vinasse formulations. Conversely, for In vitro elongation and rooting, these vinasse media were superior to KC medium. KC medium promotes a low rooting rate (8%) compared to 68 and 100% obtained by KCV1 and KCV5, respectively. Moreover, plantlets cultured on KC medium become protocorm-like body clusters, which impeded the acclimatization of these explants. Plantlets elongated and rooted on KCV1 and KCV5 were successfully acclimatized with a 91% survival rate for both KC vinasse formulations. This study shows the great potential of this technology as a rational alternative to vinasse disposal and adds value to what is currently considered a waste product. (author)

  4. Prospect of stem cell conditioned medium in regenerative medicine.

    Science.gov (United States)

    Pawitan, Jeanne Adiwinata

    2014-01-01

    Stem cell-derived conditioned medium has a promising prospect to be produced as pharmaceuticals for regenerative medicine. To investigate various methods to obtain stem cell-derived conditioned medium (CM) to get an insight into their prospect of application in various diseases. Systematic review using keywords "stem cell" and "conditioned medium" or "secretome" and "therapy." Data concerning treated conditions/diseases, type of cell that was cultured, medium and supplements to culture the cells, culture condition, CM processing, growth factors and other secretions that were analyzed, method of application, and outcome were noted, grouped, tabulated, and analyzed. Most of CM using studies showed good results. However, the various CM, even when they were derived from the same kind of cells, were produced by different condition, that is, from different passage, culture medium, and culture condition. The growth factor yields of the various types of cells were available in some studies, and the cell number that was needed to produce CM for one application could be computed. Various stem cell-derived conditioned media were tested on various diseases and mostly showed good results. However, standardized methods of production and validations of their use need to be conducted.

  5. Medium Modifications of Hadron Properties and Partonic Processes

    Energy Technology Data Exchange (ETDEWEB)

    Brooks, W. K.; Strauch, S.; Tsushima, K.

    2011-06-01

    Chiral symmetry is one of the most fundamental symmetries in QCD. It is closely connected to hadron properties in the nuclear medium via the reduction of the quark condensate , manifesting the partial restoration of chiral symmetry. To better understand this important issue, a number of Jefferson Lab experiments over the past decade have focused on understanding properties of mesons and nucleons in the nuclear medium, often benefiting from the high polarization and luminosity of the CEBAF accelerator. In particular, a novel, accurate, polarization transfer measurement technique revealed for the first time a strong indication that the bound proton electromagnetic form factors in 4He may be modified compared to those in the vacuum. Second, the photoproduction of vector mesons on various nuclei has been measured via their decay to e+e- to study possible in-medium effects on the properties of the rho meson. In this experiment, no significant mass shift and some broadening consistent with expected collisional broadening for the rho meson has been observed, providing tight constraints on model calculations. Finally, processes involving in-medium parton propagation have been studied. The medium modifications of the quark fragmentation functions have been extracted with much higher statistical accuracy than previously possible.

  6. Lensless ghost imaging through the strongly scattering medium

    International Nuclear Information System (INIS)

    Yang Zhe; Zhao Xueliang; Li Junlin; Zhao Lianjie; Qin Wei

    2016-01-01

    Lensless ghost imaging has attracted much interest in recent years due to its profound physics and potential applications. In this paper we report studies of the robust properties of the lensless ghost imaging system with a pseudo-thermal light source in a strongly scattering medium. The effects of the positions of the strong medium on the ghost imaging are investigated. In the lensless ghost imaging system, a pseudo-thermal light is split into two correlated beams by a beam splitter. One beam goes to a charge-coupled detector camera, labeled as CCD2. The other beam goes to an object and then is collected in another charge-coupled detector camera, labeled as CCD1, which serves as a bucket detector. When the strong medium, a pane of ground glass disk, is placed between the object and CCD1, the bucket detector, the quality of ghost imaging is barely affected and a good image could still be obtained. The quality of the ghost imaging can also be maintained, even when the ground glass is rotating, which is the strongest scattering medium so far. However, when the strongly scattering medium is present in the optical path from the light source to CCD2 or the object, the lensless ghost imaging system hardly retrieves the image of the object. A theoretical analysis in terms of the second-order correlation function is also provided. (paper)

  7. Quasifree η photoproduction from nuclei and medium modifications of resonances

    International Nuclear Information System (INIS)

    Ventel, B.I.S. van der; Abu-Raddad, L.J.; Hillhouse, G.C.

    2003-01-01

    This paper establishes the case that the process of quasifree η photoproduction from nuclei is an important tool to study medium modifications and changes to the elementary process γN→ηN in the nuclear medium. We investigate the sensitivity of the differential cross section, recoil nucleon polarization, and the photon asymmetry to changes in the elementary amplitude, medium modifications of the resonance (S 11 ,D 13 ) masses, as well as nuclear target effects. All calculations are performed within a relativistic plane-wave impulse approximation formalism resulting in analytical expressions for all observables. Our results indicate that polarization observables are largely insensitive to nuclear target effects. Depending on the type of coupling, the spin observables do display a sensitivity to the magnitude of the ηNN coupling constant. The polarization observables are identified to be the prime candidates to investigate the background processes and their medium modifications in the elementary process such as the D 13 resonance. Moreover, as a consequence of the large dominance in the differential cross section of the S 11 resonance, the quasifree differential cross section provides an exceptional instrument to study medium modifications to the S 11 resonance in such a manner that helps to distinguish between various models that attempt to understand the S 11 resonance and its distinctive position as the lowest lying negative parity state in the baryon spectrum

  8. Chemistry of neptunium and of other actinides in carbonate medium

    International Nuclear Information System (INIS)

    Riglet, C.

    1990-01-01

    This work is a contribution to the establishment of a thermodynamic data base in order to forecast the behaviour of Np and other actinides in natural waters that might come into contact with a nuclear waste repository. Redox potentials and complexation constants in acidic and carbonate media are measured in aqueous solutions, in the presence of an inert salt (sodium perchlorate) of variable concentrations. The activity coefficients are calculated, from the variation of thermodynamic measurements with the ionic strength, with the Specific Interaction Theory (S.I.T.). The electrochemical data, obtained by polarography and voltamperometry, are interpreted, for irreversible systems, with numerical and graphical methods based on the Koutecky-Weber's and S.I.T. equations. The equilibrium constants in carbonate medium are measured by absorption spectrophotometry and a graphical method is proposed to determine the stoichiometries of polynuclear species. In this work are proposed numerical data relative to: - the redox potentials of the systems: M(VI/V) and M(IV/III) in acidic medium where M = U, Np, Pu; U(VI/V) in carbonate medium; Np(VI/V) in bicarbonate medium of ionic strength 3 M. - the formation constants of the hexakis (carbonato) tris [dioxoneptunate (VI)] complex in 3 M medium - the formation constants of the mono, di and tri (carbonato) dioxoneptunate (V) complexes - the influence of the ionic strength on some of the studied chemical equilibria by using, when necessary, literature results. All specific interaction coefficients (activity coefficients) of these species are also measured or calculated [fr

  9. Real group velocity in a medium with dissipation

    International Nuclear Information System (INIS)

    Muschietti, L.; Dum, C.T.

    1993-01-01

    When a medium is dissipative, the classic expression for the group velocity, dω/dk, is complex with an imaginary part often being far from negligible. To clarify the role of this imaginary term, the motion of a wave packet in a dissipative, homogeneous medium is examined. The integral representation of the packet is analyzed by means of a saddle-point method. It is shown that in a moving frame attached to its maximum the packet looks self-similar. A Gaussian packet keeps its Gaussian identity, as is familiar for the case of a nondissipative medium. However, the central wave number of the packet slowly changes because of a differential damping among the Fourier components: Im(dω/dk)=dγ/dk≠0, where ω≡ω r +iγ. The packet height can be computed self-consistently as integrated damping (or growth). The real group velocity becomes a time-dependent combination of Re(dω/dk) and Im(dω/dk). Only where the medium is both homogeneous and loss free, does the group velocity remain constant. Simple ''ray-tracing equations'' are derived to follow the packet centers in coordinate and Fourier spaces. The analysis is illustrated with a comparison to geometric optics, and by two applications: the case of a medium with some resonant damping (or growth) and the propagation of whistler waves in a collisional plasma

  10. Medium effects on spin observables of proton knockout reactions

    International Nuclear Information System (INIS)

    Krein, G.; Maris, T.A.J.; Rodrigues, B.B.; Veit, E.A.

    1994-07-01

    Medium modifications of the properties of bound nucleons and mesons are investigated by means of medium energy quasi free proton knockout reactions with polarized incident protons. The sensitivity of the spin observables of these reactions to modifications of the nucleon and meson properties is studied using the Bonn one-boson exchange model of the nucleon-nucleon interaction. A method proposed to extract the pp analysing power in medium from the (p, 2 p) asymmetries indicates a reduction of this quantity compared to its free space value. This reduction is linked to modifications of masses and coupling constants of the nucleons and mesons in the nucleus. The implications of these modifications for another spin observable to be measured in the future are discussed. (author). 39 refs, 9 figs

  11. The financial process in small and medium enterprises

    Directory of Open Access Journals (Sweden)

    Mariela Andrade Arias

    2018-02-01

    Full Text Available The small medium enterprises contribute to productive apparatus of Quevedo Canton in this line of our country. These institutions count on a great number of entrepreneurs in the all productive levels. But it has generated some difficulties in the organizations’ control, even occasionally by managers’ oversight. There also are weak supports in the control and enterprise’s addressing. The present research was done with the purpose to determine the incidence of financial management is small and medium enterprises of Quevedo Canton. Empirical and theoretical methods were used and also some researching techniques such as surveys and documentary and literature review and the law and regulations of these enterprises were consulted. One of the main results was the necessity to incorporate integral training plans to improve the financial economic management of each enterprise. It concludes panting out that 74 % of small enterprises didn´t count on appropriate organizational structure to develop productive activities like the 68 % medium enterprises.

  12. Extraction of actinides from chloride medium using pentaalkylpropanediamides

    International Nuclear Information System (INIS)

    Cuillerdier, C.; Musikas, C.

    1991-01-01

    Pyrometallurgical processes for the purification of plutonium create waste solutions containing actinides, mainly americium, in chloride medium. Studies have been undertaken to study the extraction of actinides in chloride medium (hydrochloric acid mixed with concentrated salts such as LiCl, CaCl 2 , MgCl 2 , KCl) using pentaalkylpropanediamides as extractants. Plutonium (IV) is very easily extracted, Am (III) needs a salting out agent such as LiCl. Back extraction of trivalent cations is easy in HCl <5M. Plutonium(IV) and (VI) can be stripped by reduction either with ascorbic acid or hydroxylammonium salts in weak acid medium. Several diluents can be used (aromatic, chlorinated or even aliphatic) with addition of decanol to prevent third phase formation. In conclusion diamides can be used for various wastes declassification, they are potentially completely incinerable, and, as the synthesis has been optimized, they appear to be promising extractants

  13. Out of Medium Fragmentation from Long-Lived Jet Showers

    CERN Document Server

    Casalderrey-Solana, Jorge; Quiroga-Arias, Paloma

    2012-01-01

    We study the time structure of vacuum jet evolution via a simple uncertainty principle estimate in the kinematic range explored by current heavy ion collisions at the LHC. We observe that a large fraction of the partonic splittings occur at large times, of the order of several fm. We compare the time distribution of vacuum splittings with the distribution of path lengths traversed by jets in a heavy ion collision. We find that if no medium induced modification of the jet dynamics were present, a very large fraction (larger than 80% for inclusive jets) of the jet splittings would occur outside of the medium. We confront this observation with current available data on jet properties in heavy ion collisions and discuss its implications for the dynamics of jet-medium interactions.

  14. Out of medium fragmentation from long-lived jet showers

    Energy Technology Data Exchange (ETDEWEB)

    Casalderrey-Solana, Jorge, E-mail: jorge.casalderrey@cern.ch [Departament d' Estructura i Constituents de la Materia, Universitat de Barcelona, Marti i Franques 1, 08028 Barcelona (Spain); Physics Department, Theory Unit, CERN, CH-1211 Geneve 23 (Switzerland); Milhano, Jose Guilherme, E-mail: guilherme.milhano@ist.utl.pt [CENTRA, Instituto Superior Tecnico, Universidade Tecnica de Lisboa, Av. Rovisco Pais, P-1049-001 Lisboa (Portugal); Physics Department, Theory Unit, CERN, CH-1211 Geneve 23 (Switzerland); Quiroga-Arias, Paloma, E-mail: pquiroga@lpthe.jussieu.fr [LPTHE, UPMC Univ. Paris 6 and CNRR UMR7589, Paris (France)

    2012-03-29

    We study the time structure of vacuum jet evolution via a simple uncertainty principle estimate in the kinematic range explored by current heavy ion collisions at the LHC. We observe that a large fraction of the partonic splittings occur at large times, of the order of several fm. We compare the time distribution of vacuum splittings with the distribution of path lengths traversed by jets in a heavy ion collision. We find that if no medium induced modification of the jet dynamics were present, a very large fraction (larger than 80% for inclusive jets) of the jet splittings would occur outside of the medium. We confront this observation with current available data on jet properties in heavy ion collisions and discuss its implications for the dynamics of jet-medium interactions.

  15. Out of medium fragmentation from long-lived jet showers

    International Nuclear Information System (INIS)

    Casalderrey-Solana, Jorge; Milhano, José Guilherme; Quiroga-Arias, Paloma

    2012-01-01

    We study the time structure of vacuum jet evolution via a simple uncertainty principle estimate in the kinematic range explored by current heavy ion collisions at the LHC. We observe that a large fraction of the partonic splittings occur at large times, of the order of several fm. We compare the time distribution of vacuum splittings with the distribution of path lengths traversed by jets in a heavy ion collision. We find that if no medium induced modification of the jet dynamics were present, a very large fraction (larger than 80% for inclusive jets) of the jet splittings would occur outside of the medium. We confront this observation with current available data on jet properties in heavy ion collisions and discuss its implications for the dynamics of jet-medium interactions.

  16. Medium effects on spin observables of proton knockout reactions

    Energy Technology Data Exchange (ETDEWEB)

    Krein, G [Instituto de Fisica Teorica (IFT), Sao Paulo, SP (Brazil); Maris, T A.J.; Rodrigues, B B; Veit, E A [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Inst. de Fisica

    1994-07-01

    Medium modifications of the properties of bound nucleons and mesons are investigated by means of medium energy quasi free proton knockout reactions with polarized incident protons. The sensitivity of the spin observables of these reactions to modifications of the nucleon and meson properties is studied using the Bonn one-boson exchange model of the nucleon-nucleon interaction. A method proposed to extract the pp analysing power in medium from the (p, 2 p) asymmetries indicates a reduction of this quantity compared to its free space value. This reduction is linked to modifications of masses and coupling constants of the nucleons and mesons in the nucleus. The implications of these modifications for another spin observable to be measured in the future are discussed. (author). 39 refs, 9 figs.

  17. Use of medium energy particles in radiobiology and radiotherapy

    International Nuclear Information System (INIS)

    Larsson, B.

    1984-01-01

    The radiological properties of ion beams are described and their biomedical use reviewed. The special features and potentialities of secondary radiations obtained with medium-energy accelerators for protons of electrons are mentioned, for comparison. Examples are given from work at heavy accelerators which has contributed to our basic knowledge of radiation effects on cells and tissues, or to recent advances in experimental and clinical radiology. The author tries to identify areas where important spin-off contributions from medium-energy physics to the biomedical sciences could be made, or where such contributions would have significant implications for the society. The conclusion is made that medium energy accelerators have great potential in experimental and preclinical research, particularly in neurophysiology and oncology

  18. Customer relationship management implementation in the small and medium enterprise

    Science.gov (United States)

    Nugroho, Agus; Suharmanto, Agus; Masugino

    2018-03-01

    To win the global competition and sustain the business, small and medium enterprise shall implement a reliable information technology application to support their customer data base, production and sales as well as marketing management. This paper addresses the implementation of Customer Relationship Management (CRM) in small and medium enterprise, CV. Densuko Jaya. It is a small and medium enterprises in Semarang, Central Java, Republic of Indonesia deal with rubber processing industry supply chain. ADDIE model utilized in study to setup the CRM functionality at these enterprises. The aim of the authors is to present the benefits resulting from the application of CRM technologies at these enterprises to solve their chronicle issues in the field of integrated customer data base, production management process and sales automation in order to boost their business in the near future. Training and coaching have been delivered to the enterprises staffs and management to ensure that they can execute the system.

  19. Minkowski spacetime does not apply to a homogeneously accelerating medium

    Science.gov (United States)

    Coleman, Brian

    Home and comoving inertial frame parameters of an individual point of an idealized medium of launch length L uniformly co-accelerating between identical fixed-thrust rockets, are well known. This is not the case with the varying inter-rocket radar periods and related implications regarding a changing 'noninertial own-length' Λ which differs from a front rocket's retrospective separation L from the simultaneously relatively moving rear rocket. On the other hand, the nonhomogeneous acceleration case involving every comoving frame's unchanging perception of a contrived 'rigor mortis' medium (so-called 'rigid motion' traditionally associated with 'Rindler coordinates') whereby Λ = L = L , constitutes the sole extended accelerating medium scenario where the entrenched Minkowski metric is actually applicable. Paraphrasing Wolfgang Pauli, not only is Minkowski spacetime not correct [in the general sense], it is not even wrong [in the restricted sense].

  20. Pauli blocking and medium effects in nucleon knockout reactions

    International Nuclear Information System (INIS)

    Bertulani, C. A.; De Conti, C.

    2010-01-01

    We study medium modifications of the nucleon-nucleon (NN) cross sections and their influence on the nucleon knockout reactions. Using the eikonal approximation, we compare the results obtained with free NN cross sections with those obtained with a purely geometrical treatment of Pauli blocking and with NN obtained with more elaborated Dirac-Bruecker methods. The medium effects are parametrized in terms of the baryon density. We focus on symmetric nuclear matter, although the geometrical Pauli blocking also allows for the treatment of asymmetric nuclear matter. It is shown that medium effects can change the nucleon knockout cross sections and momentum distributions up to 10% in the energy range E lab =50-300 MeV/nucleon. The effect is more evident in reactions involving halo nuclei.

  1. The Interstellar Medium in External Galaxies: Summaries of contributed papers

    Science.gov (United States)

    Hollenbach, David J. (Editor); Thronson, Harley A., Jr. (Editor)

    1990-01-01

    The Second Wyoming Conference entitled, The Interstellar Medium in External Galaxies, was held on July 3 to 7, 1989, to discuss the current understanding of the interstellar medium in external galaxies and to analyze the basic physical processes underlying interstellar phenomena. The papers covered a broad range of research on the gas and dust in external galaxies and focused on such topics as the distribution and morphology of the atomic, molecular, and dust components; the dynamics of the gas and the role of the magnetic field in the dynamics; elemental abundances and gas depletions in the atomic and ionized components; cooling flows; star formation; the correlation of the nonthermal radio continuum with the cool component of the interstellar medium; the origin and effect of hot galactic halos; the absorption line systems seen in distant quasars; and the effect of galactic collisions.

  2. Focus talk on interactions between jets and medium

    International Nuclear Information System (INIS)

    Ruppert, Joerg

    2006-01-01

    The energy and momentum lost by a hard parton propagating through hot and dense matter has to be redistributed during the nuclear medium evolution. Apart from heating the medium, there is the possibility that collective modes are excited leading to the emergence of Mach cones or Cherenkov radiation. Recent two-particle correlation measurements by STAR [F. Wang [STAR Collaboration], J. Phys. G 30, S1299 (2004) [arXiv:nucl-ex/0404010]; C. Gagliardi, these proceedings] and PHENIX [S. S. Adler et al. [PHENIX Collaboration], arXiv:nucl-ex/0507004; N. Ajitanand, these proceedings] at RHIC indicate that such phenomena may play an important role in understanding the jet-medium interactions. Possible collective modes are discussed and it is demonstrated that Mach cones as created by colorless or colored sound are a possible explanation of the hardronic two-particle correlation data

  3. The interaction of supernova ejecta with an ambient medium

    International Nuclear Information System (INIS)

    Chevalier, R.A.

    1983-01-01

    Plausible environments for supernovae are the interstellar medium with constant density or a circumstellar medium built up by mass loss with rho proportional to r -2 . Self-similar solutions for the interaction region between the expanding supernova gas and the ambient gas exist provided that the expanding gas has rho proportional to rsup(-n) with n > 5. The circumstellar medium case is likely to be important for the early evolution of Type II supernovae because their progenitor stars are probably red supergiants. The radio and X-ray emission observed from extragalactic supernovae may be from this interaction region. The early self-similar solutions can also be applied to the young galactic remnants. (Auth.)

  4. Extruder system and method for treatment of a gaseous medium

    Energy Technology Data Exchange (ETDEWEB)

    Silvi, Norberto; Perry, Robert James; Singh, Surinder Prabhjot; Balch, Gary Stephen; Westendorf, Tiffany Elizabeth Pinard

    2016-04-05

    A system for treatment of a gaseous medium, comprises an extruder having a barrel. The extruder further comprises a first inlet port, a second inlet port, and a plurality of outlet ports coupled to the barrel. The first inlet port is configured for feeding a lean sorbent, the second inlet port is configured for feeding a gaseous medium, and the plurality of outlet ports are configured for releasing a plurality of components removed from the gaseous medium. Further, the extruder comprises a plurality of helical elements coupled to a plurality of kneading elements, mounted on a shaft, and disposed within the barrel. The barrel and the plurality of helical and kneading elements together form an absorption unit and a desorption unit. The first and second inlet ports are formed in the absorption unit and the plurality of outlet ports are formed in the absorption and desorption units.

  5. Self-consistency of a heterogeneous continuum porous medium representation of a fractured medium

    International Nuclear Information System (INIS)

    Hoch, A.R.; Jackson, C.P.; Todman, S.

    1998-01-01

    For many of the rocks that are, or have been, under investigation as potential host rocks for a radioactive waste repository, groundwater flow is considered to take place predominantly through discontinuities such as fractures. Although models of networks of discrete features (DFN models) would be the most realistic models for such rocks, calculations on large length scales would not be computationally practicable. A possible approach would be to use heterogeneous continuum porous-medium (CPM) models in which each block has an effective permeability appropriate to represent the network of features within the block. In order to build confidence in this approach, it is necessary to demonstrate that the approach is self-consistent, in the sense that if the effective permeability on a large length scale is derived using the CPM model, the result is close to the value derived directly from the underlying network model. It is also desirable to demonstrate self-consistency for the use of stochastic heterogeneous CPM models that are built as follows. The correlation structure of the effective permeability on the scale of the blocks is inferred by analysis of the effective permeabilities obtained from the underlying DFN model. Then realizations of the effective permeability within the domain of interest are generated on the basis of the correlation structure, rather than being obtained directly from the underlying DFN model. A study of self-consistency is presented for two very different underlying DFN models: one based on the properties of the Borrowdale Volcanic Group at Sellafield, and one based on the properties of the granite at Aespoe in Sweden. It is shown that, in both cases, the use of heterogeneous CPM models based directly on the DFN model is self-consistent, provided that care is taken in the evaluation of the effective permeability for the DFN models. It is also shown that the use of stochastic heterogeneous CPM models based on the correlation structure of the

  6. Improved lipid and biomass productivities in Chlorella vulgaris by differing the inoculation medium from the production medium

    Directory of Open Access Journals (Sweden)

    Shahrbanoo Hamedi

    2016-06-01

    Full Text Available Improvement of biomass and lipid productivities is now one of the main concerns in commercialization of microalgae cultivation as a feedstock for algal biofuel production. Conventional photoautotrophic processes using well-studied and rich in oil strain of Chlorella vulgaris are not able to meet such demands. A new strategy of inoculating algae production medium with cells grown in a different medium from the production medium was proposed herein. More specifically, when SH4 was used as production medium and N8 was used as inoculation medium, biomass and lipid productivities increased by 2.33 folds and 1.44 fold, respectively, compared with when the production and inoculation media were the same, such as SH4. The findings of the present investigation showed that this cultivation scheme resulted in 52% increase in cell number and 54% increase in dry weight leading to improved productivities. Although by even considering this improvement, photoautotrophic cultivation of algae can hardly compete with the heterotrophic cultivation, the high cost of hydrocarbon supply required in large-scale heterotrophic processes marks the technique proposed in the present study as a promising approach for commercialization of algal biofuel production.

  7. Apps: a new medium for non-fiction innovation

    Directory of Open Access Journals (Sweden)

    Catherine Allen

    2015-07-01

    Full Text Available Apps are now a dominant content medium: in the US people spend more time on apps than they do watching TV. Non-fiction content is being avidly consumed on mobile devices, but in a completely different way to the book model. This article explores three strands of potential that the app medium holds for non-fiction content, putting forward the case that apps have the power to further weave non-fiction into the fabric of society and life.

  8. Experimental studies on solar parabolic dish cooker with porous medium

    International Nuclear Information System (INIS)

    Lokeswaran, S.; Eswaramoorthy, M.

    2012-01-01

    The solar cooking is the alternate method of cooking to reduce consumptions of fossil fuels. An affordable, energy efficient solar cooking technology is much need due to the fossil fuels increasing cost and it is the hottest research topic in all over the world. This paper presents an experimental analysis of the heat transfer enhancement of solar parabolic dish cookers by a porous medium made of scrap material. Using the stagnation temperature test and water boiling test are conducted on the cooking vessel with and without porous medium. Experimental results are compared for both cases in terms of thermal performance, optical efficiency, heat loss factor and cooking power. (authors)

  9. Medium-term load forecasting and wholesale transaction profitability

    International Nuclear Information System (INIS)

    Selker, F.K.; Wroblewski, W.R.

    1996-01-01

    The volume of wholesale transactions quoted at firm prices is increasing. The cost, and thus profitability, of serving these contracts strongly depends upon native load during the time of delivery. However, transactions extend beyond load forecasts based on weather information, and long-term resource planning forecasts of load peaks and energy provide inadequate detail. To address this need, Decision Focus Inc. (DFI) and Commonwealth Edison (ComEd) developed a probabilistic, medium-term load forecasting capability. In this paper the authors use a hypothetical utility to explore the impact of uncertain medium-term loads on transaction profitability

  10. Binary circular inclusions in an effective medium approximation

    Energy Technology Data Exchange (ETDEWEB)

    Alexopoulos, A. [Electronic Warfare and Radar Division, Defence Science and Technology Organisation (DSTO), P.O. Box 1500, Edinburgh 5111 (Australia)]. E-mail: aris.alexopoulos@dsto.defence.gov.au

    2005-05-02

    We investigate the second order interactions of perfectly-conducting circular inclusions embedded in a medium of dielectric constant {epsilon}{sub 0} under the influence of an electric field E{sub 0}. Using dipole theory we show that the potential difference for the composite system is written in terms of complicated continued fractions. The form of these continued fractions is rederived in terms of simple square matrices which simplify the computationally intense interaction terms between the two inclusions. Asymptotic expressions are derived from the continued fractions that are then used to calculate the conductance of the medium.

  11. Medium-term marginal costs in competitive generation power markets

    International Nuclear Information System (INIS)

    Reneses, J.; Centeno, E.; Barquin, J.

    2004-01-01

    The meaning and significance of medium-term marginal costs for a generation company in a competitive power market in analysed. A methodology to compute and decompose medium-term generation marginal costs in a competitive environment is proposed. The methodology is based on a market equilibrium model. The aim is to provide a useful tool for generation companies so that they can manage their resources in an optimal way, helping them with their operation, decision-making processes, asset valuations or contract assessments. (author)

  12. Scattering theory and effective medium approximations to heterogeneous materials

    International Nuclear Information System (INIS)

    Gubernatis, J.E.

    1977-01-01

    The formal analogy existing between problems studied in the microscopic theory of disordered alloys and problems concerned with the effective (macroscopic) behavior of heterogeneous materials is discussed. Attention is focused on (1) analogous approximations (effective medium approximations) developed for the microscopic problems by scattering theory concepts and techniques, but for the macroscopic problems principally by intuitive means, (2) the link, provided by scattering theory, of the intuitively developed approximations to a well-defined perturbative analysis, (3) the possible presence of conditionally convergent integrals in effective medium approximations

  13. Safety and licensing for small and medium power reactors

    International Nuclear Information System (INIS)

    Trauger, D.B.

    1987-01-01

    Proposed new concepts for small and medium power reactors differ substantially from traditional Light Water Reactors (LWRs). Although designers have a large base of experience in safety and licensing, much of it is not relevant to new concepts. It can be a disadvantage if regulators apply LWR rules directly. A fresh start is appropriate. The extensive interactions between industry, regulators, and the public complicates but may enhance safety. It is basic to recognize the features that distinguish nuclear energy safety from that for other industries. These features include: nuclear reactivity, fission product radiation, and radioactive decay heat. Small and medium power reactors offer potential advantages over LWRs, particularly for reactivity and decay heat

  14. In-medium effects around the Fermi energy

    Directory of Open Access Journals (Sweden)

    Lopez O.

    2015-01-01

    Full Text Available We study nuclear stopping in central collisions for heavy-ion induced reactions in the Fermi energy domain (15-100 AMeV. Using the large dataset provided by the 4π array INDRA, we determine that stopping can be directly related to the transport properties in the nuclear medium. By looking specifically at protons, we present a comprehensive body of experimental results concerning the mean free path, the nucleon-nucleon cross-section and in-medium effects in nuclear matter.

  15. Dynamics of continuous medium in space with torsion

    International Nuclear Information System (INIS)

    Krechet, V.G.

    1985-01-01

    In frames of Einstein-Cartan gravitation theory general properties of continuous media dynamics using description formalism of continuous medium steam-line congruence geometry are investigated. Raichaudhuri type equations in space with torsion applied to study the problem of singularities in gravitation theory are derived. It is shown that space-time torsion tensor trace may immediately affect volumetric autoparallel divergence and torsion pseudo trace - rotation of continuous medium steam-line congruences. Using formalism considered metrics of homogeneous rotation nonstationary cosmological model is determined and investigated

  16. Precompound decay models for medium energy nuclear reactions

    International Nuclear Information System (INIS)

    Blann, M.

    1989-11-01

    The formulations used for precompound decay models are presented and explained in terms of the physics of the intranuclear cascade model. Several features of spectra of medium energy (10--1000 MeV) reactions are summarized. Results of precompound plus evaporation calculations from the code ALICE are compared with a wide body of proton, alpha, and heavy ion induced reaction data to illustrate both the power and deficiencies of predicting yield of these reactions in the medium energy regime. 23 refs., 13 figs

  17. English Medium Instruction in Multilingual and Multicultural Universities:

    DEFF Research Database (Denmark)

    Henriksen, Birgit; Holmen, Anne; Kling, Joyce

    ’ experiences in the midst of curricular change and presents reflections on ways to professionally navigate in English to meet the demands of the multilingual and multicultural classroom. English Medium Instruction in Multilingual and Multicultural Universities is key reading for university management......English Medium Instruction in Multilingual and Multicultural Universities analyses the issues related to EMI at both a local and international level and provides a broad perspective on this topic. Drawing on field studies from a Northern European context and based primarily on research carried out...

  18. Medium carbon vanadium micro alloyed steels for drop forging

    International Nuclear Information System (INIS)

    Jeszensky, Gabor; Plaut, Ronald Lesley

    1992-01-01

    Growing competitiveness of alternative manufacturing routes requires cost minimization in the production of drop forged components. The authors analyse the potential of medium carbon, vanadium microalloyed steels for drop forging. Laboratory and industrial experiments have been carried out emphasizing deformation and temperature cycles, strain rates and dwell times showing a typical processing path, associated mechanical properties and corresponding microstructures. The steels the required levels of mechanical properties on cooling after forging, eliminating subsequent heat treatment. The machinability of V-microalloyed steels is also improved when compared with plain medium carbon steels. (author)

  19. Assessment of the world market for small and medium reactors

    International Nuclear Information System (INIS)

    Csik, B.J.

    2000-01-01

    In the past decades, the major focus for nuclear power has been the design and construction of nuclear plants of ever increasing size. This was appropriate for many industrialized countries, which could readily add generation capability to their electrical grids in large increments. However, recently there has been an increasing emphasis on the development of small and medium reactors especially to meet needs in developing countries where electrical grids cannot accept the additional capacity of a large nuclear plant. The paper presents an estimation of the world market for small and medium sized reactors giving the basic assumptions, criteria, scope, methods and important factors. (author)

  20. Multipole electromagnetic moments of neutrino in dispersive medium

    International Nuclear Information System (INIS)

    Semikov, V.B.; Smorodinskij, Ya.A.; Gosudarstvennyj Komitet po Ispol'zovaniyu Atomnoj Ehnergii SSSR, Moscow

    1989-01-01

    Four multipole moments for a Dirac and Majorana neutrino in a dispersive medium are calculated viz., the electric monopole (charge), electric dipole, magnetic dipole and anapole dipole moment. For comparison the same quantities are presented in the case of vacuum. The neutrino does not possess an (induced) anapole moment in an isotropic medium; however, in a ferromagnetic such a moment exists and for the Majorana neutrino it is the only electromagnetic cjaracteristic. As an example the cross section for elastic scattering of a Majorana neutrino by nuclei in an isotropic plasma is calculated

  1. Measuring medium-induced gluons via jet grooming

    Science.gov (United States)

    Tywoniuk, Konrad; Mehtar-Tani, Yacine

    2017-11-01

    Jet substructure observables and applications of jet grooming techniques in heavy-ion collisions are still in its infancy and provide new alleys for studying medium modifications of perturbative degrees of freedom. We note that these measurements, given the right transverse momentum range, can be uniquely sensitive to rare medium-induced emissions inside of the jet cone. This corresponds to an infrared enhancement that would, for instance, affect the distribution of the groomed momentum-sharing variable zg measured using the SoftDrop procedure.

  2. Safety Analysis for Medium/Small Size Integral Reactor: Evaluation of Safety Characteristics for Small and Medium Integral Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hho jung; Seul, K W; Ahn, S K; Bang, Y S; Park, D G; Kim, B K; Kim, W S; Lee, J H; Kim, W K; Shim, T M; Choi, H S; Ahn, H J; Jung, D W; Kim, G I; Park, Y M; Lee, Y J [Korea Inst. of Nuclear Safety, Taejon (Korea, Republic of)

    1997-07-01

    The Small and medium integral reactor is developed to be utilized for non-electric areas such as district heating and steam production for desalination and other industrial purposes, and then these applications may typically imply a closeness between the reactor and the user. It requires the reactor to be designed with the adoption of special functional and inherent safety features to ensure and promote a high level of safety and reliability, in comparison with the existing nuclear power plants. The objective of the present study is to establish the bases for the development of regulatory requirements and technical guides to address the special safety characteristics of the small and medium integral reactor. In addition, the study aims to identify and to propose resolutions to the possible safety concerns in the design of the small and medium integral reactor. 34 refs., 20 tabs. (author)

  3. Probing the mechanisms of drug release from amorphous solid dispersions in medium-soluble and medium-insoluble carriers.

    Science.gov (United States)

    Sun, Dajun D; Lee, Ping I

    2015-08-10

    The objective of the current study is to mechanistically differentiate the dissolution and supersaturation behaviors of amorphous drugs from amorphous solid dispersions (ASDs) based on medium-soluble versus medium-insoluble carriers under nonsink dissolution conditions through a direct head-to-head comparison. ASDs of indomethacin (IND) were prepared in several polymers which exhibit different solubility behaviors in acidic (pH1.2) and basic (pH7.4) dissolution media. The selected polymers range from water-soluble (e.g., PVP and Soluplus) and water-insoluble (e.g., ethylcellulose and Eudragit RL PO) to those only soluble in an acidic or basic dissolution medium (e.g., Eudragit E100, Eudragit L100, and HPMCAS). At 20wt.% drug loading, DSC and powder XRD analysis confirmed that the majority of incorporated IND was present in an amorphous state. Our nonsink dissolution results confirm that whether the carrier matrix is medium soluble determines the release mechanism of amorphous drugs from ASD systems which has a direct impact on the rate of supersaturation generation, thus in turn affecting the evolution of supersaturation in amorphous systems. For example, under nonsink dissolution conditions, the release of amorphous IND from medium-soluble carriers is governed by a dissolution-controlled mechanism leading to an initial surge of supersaturation followed by a sharp decline in drug concentration due to rapid nucleation and crystallization. In contrast, the dissolution of IND ASD from medium-insoluble carriers is more gradual as drug release is regulated by a diffusion-controlled mechanism by which drug supersaturation is built up gradually and sustained over an extended period of time without any apparent decline. Since several tested carrier polymers can be switched from soluble to insoluble by simply changing the pH of the dissolution medium, the results obtained here provide unequivocal evidence of the proposed transition of kinetic solubility profiles from the

  4. Poster presentation - a visual medium for academic and scientific meetings.

    Science.gov (United States)

    Rowe, Nicholas; Ilic, Dragan

    2011-09-01

    Academic poster presentations are used as a medium of knowledge transfer by a wide range of health professional groups. Posters also provide a means of publication for academic and professional contributors. Posters are designed to give a visual representation of an issue that firstly attracts attention, and then conveys an intended message. Whilst the poster medium has become adopted into the publication orthodoxy of the scientific and academic communities, there are acknowledged limitations regarding the depth of knowledge transfer, issues of compilation and production, and the related viewer conception. If treated as a standalone medium, the limitations of a two-dimensional, page limited format may not provide the ideal opportunity to deliver the depth of information required within the academic context. Despite these limitations however, the continued use of posters is justified when supported by author presentation or multi-media resources. This paper aims to provide an overview of the current concept and practicality of academic poster publications. It also outlines by example, some of the wider principles of poster compilation and presentation, for use by those who may utilise the medium at academic and scientific meetings. Copyright © 2011 Elsevier Ltd. All rights reserved.

  5. Estimated spatial requirements of the medium- to large-sized ...

    African Journals Online (AJOL)

    Conservation planning in the Cape Floristic Region (CFR) of South Africa, a recognised world plant diversity hotspot, required information on the estimated spatial requirements of selected medium- to large-sized mammals within each of 102 Broad Habitat Units (BHUs) delineated according to key biophysical parameters.

  6. Nuclear reactions of high energy deuterons with medium mass targets

    International Nuclear Information System (INIS)

    Numajiri, Masaharu; Miura, Taichi; Oki, Yuichi

    1994-01-01

    Formation cross sections of product nuclides in the nuclear reactions of medium mass targets by 10 GeV deuterons were measured with a gamma-ray spectroscopy. The measured data were compared with the cross sections of 12 GeV protons. (author)

  7. A Systematic Review of English Medium Instruction in Higher Education

    Science.gov (United States)

    Macaro, Ernesto; Curle, Samantha; Pun, Jack; An, Jiangshan; Dearden, Julie

    2018-01-01

    After outlining why a systematic review of research in English medium instruction (EMI) in higher education (HE) is urgently required, we briefly situate the rapidly growing EMI phenomenon in the broader field of research in which content and language have been considered and compare HE research outputs with those from other phases of education.…

  8. Genetic Basis for Saccharomyces cerevisiae Biofilm in Liquid Medium

    DEFF Research Database (Denmark)

    Andersen, Kaj Scherz; Bojsen, Rasmus Kenneth; Gro Rejkjær Sørensen, Laura

    2014-01-01

    Biofilm-forming microorganisms switch between two forms: free-living planktonic and sessile multicellular. Sessile communities of yeast biofilms in liquid medium provide a primitive example of multicellularity and are clinically important because biofilms tend to have other growth characteristics...

  9. Uncovering English-medium instruction glocal issues in higher education

    CERN Document Server

    Drljaca Margic, Branka

    2016-01-01

    This book draws on a range of theoretical and empirical insights to explore the implications of English-medium instruction in higher education and how to capitalize on its strengths and respond to its challenges. It opens up new avenues for research relevant to all educational institutions undergoing change in this field.

  10. Scattering amplitude and shift in self-energy in medium

    International Nuclear Information System (INIS)

    Mallik, S.

    2002-01-01

    Two simple proofs are presented for the first order virial expansion of the self-energy of a particle moving through a medium, characterised by temperature and/or chemical potential(s). One is based on the virial expansion of the self-energy operator itself, while the other is based on the analysis of its Feynman diagrams in configuration space. (orig.)

  11. Vanadium Effect on a Medium Carbon Forging Steel

    Directory of Open Access Journals (Sweden)

    Carlos Garcia-Mateo

    2016-05-01

    Full Text Available In the present work the influence of vanadium on the hardenability and the bainitic transformation of a medium carbon steel is analyzed. While V in solid solution enhances the former, it hardly affects bainitic transformation. The results also reveal an unexpected result, an increase of the prior austenite grain size as the V content increases.

  12. Statistical properties of laser light scattering in Brownian medium

    International Nuclear Information System (INIS)

    Suwono; Santoso, Budi; Baiquni, A.

    1983-01-01

    Relationship between statistical properties of laser light scattering in Brownian medium and photon-counting distributions are described in detail. A coherence optical detection has been constructed and by using photon-counting technique the ensemble distribution of the scattered field within space and time coherence has been measured. Good agreement between theory and experiment is shown. (author)

  13. Electromagnetic radiation in a time-varying background medium

    NARCIS (Netherlands)

    Budko, N.V.

    2009-01-01

    Analytical solutions are presented for the electromagnetic radiation by an arbitrary pulsed source into a homogeneous time-varying background medium. In the constant-impedance case an explicit radiation formula is obtained for the synchronous permittivity and permeability described by any positive

  14. Electromagnetic Radiation in a Uniformly Moving, Homogeneous Medium

    DEFF Research Database (Denmark)

    Johannsen, Günther

    1972-01-01

    A new method of treating radiation problems in a uniformly moving, homogeneous medium is presented. A certain transformation technique in connection with the four-dimensional Green's function method makes it possible to elaborate the Green's functions of the governing differential equations...

  15. Calophyllum inophyllum L. of Clusiaceae is a handsome medium ...

    Indian Academy of Sciences (India)

    Calophyllum inophyllum L. of Clusiaceae is a handsome medium-sized evergreen tree. The leaves are shiny with many fine parallel lateral veins. Flowers in clusters are white, fragrant and bear many stamens. Fruit is hard, subglobose and little smaller than a golf ball. Bark astringent, sweet and diuretic. Seed oil is used ...

  16. Glass foam granulate as growing medium for tomato and cucumber

    NARCIS (Netherlands)

    Blok, C.; Winkel, van A.; Chizhmak, S.

    2011-01-01

    Glass foam granulate was evaluated for use as a horticultural rooting medium with laboratory tests and cultivation experiments. The laboratory tests included moisture characteristics, rehydration rate and pH buffering analyses. Cucumbers and later on tomatoes were propagated in rockwool propagation

  17. Film as a "Thoughtful" Medium for Teaching History

    Science.gov (United States)

    Stoddard, Jeremy D.

    2012-01-01

    This collective case study of teachers and students in two ninth-grade US history classes examines the role that films can play as a "thoughtful" medium for teaching history. Specifically, the study focuses on the nature and range of authentic intellectual work that students are engaged in with film in the classroom (Newmann, F., B.…

  18. Field equipotentials of a fast-moving charge in medium

    International Nuclear Information System (INIS)

    Strel'tsov, V.N.

    1994-01-01

    The Lienard-Wiechert field equipotentials of an uniformly moving charge in medium are presented. It is stressed that the obtained curves describe in fact the angular dependence if formation ways of the radiation. In particular, the Cherenkov radiation corresponds to the infinite formation way. 7 refs.; 1 fig. (author)

  19. Extravasation of contrast medium into the gastrointestinal tract following lymphangiography

    International Nuclear Information System (INIS)

    Mihara, K.; Koga, K.; Tsurudome, H.; Nakano, T.; Hoshi, H.; Yamada, H.; Kawahira, K.; Inakura, M.; Watanabe, K.; Haraguchi, Y.

    1981-01-01

    Two cases with roentgenologic findings of extravasation of contrast medium into the stomach and colon following lymphangiography are presented. One is clinically diagnosed as primary intestinal lymphangiectasia; the other as retroperitoneal spread from uterine cancer. The significance of lymphangiography in gastrointestinal or retroperitoneal disorders is discussed. (orig.)

  20. Extravasation of contrast medium into the gastrointestinal tract following lymphangiography

    Energy Technology Data Exchange (ETDEWEB)

    Mihara, K.; Koga, K.; Tsurudome, H.; Nakano, T.; Hoshi, H.; Yamada, H.; Kawahira, K.; Inakura, M.; Watanabe, K.; Haraguchi, Y.

    1981-07-15

    Two cases with roentgenologic findings of extravasation of contrast medium into the stomach and colon following lymphangiography are presented. One is clinically diagnosed as primary intestinal lymphangiectasia; the other as retroperitoneal spread from uterine cancer. The significance of lymphangiography in gastrointestinal or retroperitoneal disorders is discussed.

  1. Population level response of downy brome to soil growing medium

    Science.gov (United States)

    Downy brome (Bromus tectorum) is the most ubiquitous exotic invasive weed in the Intermountain West. A major issue for management is the extreme generalist plastic nature of downy brome. We hypothesized that soil growing medium would effect all measured response variables representing some degree of...

  2. Optical variability of the medium-bright quasar sample

    International Nuclear Information System (INIS)

    Huang, K.; Mitchell, K.J.; Usher, P.D.

    1990-01-01

    A variability study of the 32-member Medium-Bright Quasar Sample is reported. It is found that the star US 1953 has undergone a noticeable variation in the course of 26 hr. Apparent variations in the extragalactic object US 3498 may be illusory, owing to its partially resolved appearance. No other evidence for variability was detected. 34 refs

  3. Cellular and physiological effects of medium-chain triglycerides.

    NARCIS (Netherlands)

    Wanten, G.J.A.; Naber, A.H.J.

    2004-01-01

    From a nutritional standpoint, saturated triglycerides with a medium (6 to 12) carbon chain length (MCT) have traditionally been regarded as biologically inert substances, merely serving as a source of fuel calories that is relatively easily accessible for metabolic breakdown compared with long

  4. DIRECTIONS OF DEVELOPMENT OF SMALL AND MEDIUM ENTERPRISES IN POLAND

    Directory of Open Access Journals (Sweden)

    Joanna Szwacka-Mokrzycka

    2013-09-01

    Full Text Available The private sector, dominated by small and medium enterprises (SME, has been rapidly developing in Poland. Small and medium enterprises are defined as economic entities hiring not more than 250 employees and have turnovers lower than 50 million euros per year. The functioning of small and medium enterprises supports a decrease in unemployment, contributes to introduction of innovative operations and products as well as significantly influences GDP generation. The SME sector faces many barriers limiting their development. They include mainly social, financial and law barriers. Operations of the Polish Agency for Enterprise Development, Academic Business Incubators and other entities support small entrepreneurship. Recently, marketing in small and medium enterprises has particularly influenced their survival. A marketing programme consists of four elements a product, price, distribution, and promotion and is described as the marketing mix. In the paper, results of research in randomly selected enterprises of the SME sector are presented. The aim of the research was to diagnose a range of marketing operations’ use in these companies. The conducted research displays that marketing activity is used mainly for promotion in the investigated enterprises and to a small extent for managing a product’s portfolio in a firm. 

  5. Probing the nuclear medium with the K+ meson

    International Nuclear Information System (INIS)

    Chrien, R.E.

    1995-01-01

    Elastic differential cross sections for K + mesons scattered from targets of carbon and 6 Li have been measured at an incident momentum of 715 MeV/c. The ratios of scattering cross sections from these targets are not predicted by theory, and are consistent with earlier suggestions that the K + -nucleon interaction is modified in the nuclear medium

  6. Stator insulation systems for medium voltage PWM drives fed motors

    International Nuclear Information System (INIS)

    Gao, G.; Chen, W.

    2005-01-01

    This paper presents the partial results of a research project that studied the impact of medium voltage PWM ASD (adjustable speed drives) on motor stator insulation system. The findings from this study/ investigation have aided designers to improve the robustness of the insulation system used for ASD-fed motors, based on accelerated laboratory tests. (author)

  7. Medium-chain acyl-CoA dehydrogenase deficiency

    DEFF Research Database (Denmark)

    Waddell, Leigh; Wiley, Veronica; Carpenter, Kevin

    2006-01-01

    The fatty acid oxidation disorder most commonly identified by tandem mass spectrometry newborn screening is the potentially fatal medium-chain acyl-CoA dehydrogenase deficiency (MCAD). In clinically presenting cases, 80% are homozygous for the common mutation, c.985A > G and 18% heterozygous. We ...

  8. Extravasation of contrast medium during CT examination: An ...

    African Journals Online (AJOL)

    2013-03-31

    Extravasation of contrast medium during CT examination: An observational casecontrol study. ... Methods: every incident of extravasation which occurred between March 2012 and March 31, 2013 was recorded in an extravasation form. Ethics Committee approval was obtained and the patients gave their consent to ...

  9. Medium Access Control in Energy Harvesting - Wireless Sensor Networks

    DEFF Research Database (Denmark)

    Fafoutis, Xenofon

    Focusing on Wireless Sensor Networks (WSN) that are powered by energy harvesting, this dissertation focuses on energy-efficient communication links between senders and receivers that are alternating between active and sleeping states of operation. In particular, the focus lies on Medium Access...

  10. Workplace Health Promotion within Small and Medium-Sized Enterprises

    Science.gov (United States)

    Moore, Ann; Parahoo, Kader; Fleming, Paul

    2010-01-01

    Purpose: The purpose of this study is to explore managers' understanding of workplace health promotion (WHP) and experiences of WHP activity within small and medium-sized enterprises (SMEs) in a Health and Social Care Trust area of Northern Ireland. The paper aims to focus on engagement with activities within the context of prevention of…

  11. Minkowski spacetime does not apply to a homogeneously accelerating medium

    Directory of Open Access Journals (Sweden)

    Brian Coleman

    Full Text Available Home and comoving inertial frame parameters of an individual point of an idealized medium of launch length L uniformly co-accelerating between identical fixed-thrust rockets, are well known. This is not the case with the varying inter-rocket radar periods and related implications regarding a changing ‘noninertial own-length’ Λ which differs from a front rocket’s retrospective separation L from the simultaneously relatively moving rear rocket. On the other hand, the nonhomogeneous acceleration case involving every comoving frame’s unchanging perception of a contrived ‘rigor mortis’ medium (so-called ‘rigid motion’ traditionally associated with ‘Rindler coordinates’ whereby Λ=L=L, constitutes the sole extended accelerating medium scenario where the entrenched Minkowski metric is actually applicable. Paraphrasing Wolfgang Pauli, not only is Minkowski spacetime not correct [in the general sense], it is not even wrong [in the restricted sense]. Keywords: Homogeneous acceleration, Radar intervals, Minkowski metric, Rigor mortis acceleration, Medium-timed photon crossing rate, Retrospective separation

  12. Medium optimization for protopectinase production by batch culture ...

    African Journals Online (AJOL)

    ajl yemi

    2011-11-11

    Nov 11, 2011 ... Optimization of medium compositions for protopectinase production by ... food industry, pharmacy and cosmetic manufacture due to ... energy intensive and industrial wastes (Iglesias and ... group of enzymes, which produce the enzymatic ... development time and overall costs (Pan et al., 2008; Ren.

  13. 21 CFR 866.2390 - Transport culture medium.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Transport culture medium. 866.2390 Section 866.2390 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2390 Transport culture...

  14. Diffusion in one dimensional random medium and hyperbolic Brownian motion

    International Nuclear Information System (INIS)

    Comtet, A.; Monthus, C.; Paris-6 Univ., 75

    1995-03-01

    Classical diffusion in a random medium involves an exponential functional of Brownian motion. This functional also appears in the study of Brownian diffusion on a Riemann surface of constant negative curvature. This relationship is analyzed in detail and various distributions are studied using stochastic calculus and functional integration. (author) 17 refs

  15. Genetic Basis for Saccharomyces cerevisiae Biofilm in Liquid Medium

    DEFF Research Database (Denmark)

    Andersen, Kaj Scherz; Bojsen, Rasmus Kenneth; Gro Rejkjær Sørensen, Laura

    2014-01-01

    than free-living cells. We investigated the genetic basis for yeast, Saccharomyces cerevisiae, biofilm on solid surfaces in liquid medium by screening a comprehensive deletion mutant collection in the S1278b background and found 71 genes that were essential for biofilm development. Quantitative...

  16. Growth medium for the rapid isolation and identification of anthrax

    Science.gov (United States)

    Kiel, Johnathan L.; Parker, Jill E.; Grubbs, Teri R.; Alls, John L.

    2000-07-01

    Anthrax has been recognized as a highly likely biological warfare or terrorist agent. The purpose of this work was to design a culture technique to rapidly isolate and identify `live' anthrax. In liquid or solid media form, 3AT medium (3-amino-L-tyrosine, the main ingredient) accelerated germination and growth of anthrax spores in 5 to 6 hours to a point expected at 18 to 24 hours with ordinary medium. During accelerated growth, standard definitive diagnostic tests such as sensitivity to lysis by penicillin or bacteriophage can be run. During this time, the bacteria synthesized a fluorescent and thermochemiluminescent polymer. Bacteria captured by specific antibody are, therefore, already labeled. Because living bacteria are required to generate the polymer, the test converts immunoassays for anthrax into viability assays. Furthermore, the polymer formation leads to the death of the vegetative form and non-viability of the spores produced in the medium. By altering the formulation of the medium, other microbes and even animal and human cells can be grown in it and labeled (including viruses grown in the animal or human cells).

  17. Orientations towards English among English-Medium Instruction Students

    Science.gov (United States)

    Karakas, Ali

    2015-01-01

    Based on the empirical data of my PhD research, this paper analyses the perceptions of 351 undergraduate students enrolled at English-medium universities towards English in terms of the language ideology framework. The students were purposively sampled from three programs at three Turkish universities. The data were drawn from student opinion…

  18. Dry processing versus dense medium processing for preparing thermal coal

    CSIR Research Space (South Africa)

    De Korte, GJ

    2013-10-01

    Full Text Available of the final product. The separation efficiency of dry processes is, however, not nearly as good as that of dense medium and, as a result, it is difficult to effectively beneficiate coals with a high near-dense content. The product yield obtained from some raw...

  19. Architecture of Institution & Home. Architecture as Cultural Medium

    NARCIS (Netherlands)

    Robinson, J.W.

    2004-01-01

    This dissertation addresses how architecture functions as a cultural medium. It does so by by investigating how the architecture of institution and home each construct and support different cultural practices. By studying the design of ordinary settings in terms of how qualitative differences in

  20. Medium term results of Avon patellofemoral joint replacement

    Directory of Open Access Journals (Sweden)

    Praveen K Sarda

    2011-01-01

    Conclusion: The Avon patellofemoral joint replacement provides predictably good results and excellent survivorship in the medium term, for isolated patellofemoral arthritis. However, progression of tibiofemoral arthritis remains unpredictable and therefore patient selection is crucial to ensure success. Clicking remains a potential problem and can compromise the postoperative results in upto 15% of the cases.

  1. Sorbitol dehydration into isosorbide in a molten salt hydrate medium

    NARCIS (Netherlands)

    Li, J.; Spina, A.; Moulijn, J.A.; Makkee, M.

    2013-01-01

    The sorbitol conversion in a molten salt hydrate medium (ZnCl2; 70 wt% in water) was studied. Dehydration is the main reaction, initially 1,4- and 3,6-anhydrosorbitol are the main products that are subsequently converted into isosorbide; two other anhydrohexitols, (1,5- and 2,5-), formed are in less

  2. Prokaryotic communities in drinking water biofilters using alternative filter medium

    DEFF Research Database (Denmark)

    Breda, Inês Lousinha Ribeiro; Roslev, Peter; Ramsay, Loren

    in an alternative filter medium during the start-up of manganese removal. Filter media properties were measured using gravimetric methods and a photometric particle analyzer. Physical, chemical and microbial analyses were used to follow the manganese ripening. Microbial analyses of both inlet water and filter...

  3. Influence of the medium's dimensionality on defect-mediated turbulence.

    Science.gov (United States)

    St-Yves, Ghislain; Davidsen, Jörn

    2015-03-01

    Spatiotemporal chaos in oscillatory and excitable media is often characterized by the presence of phase singularities called defects. Understanding such defect-mediated turbulence and its dependence on the dimensionality of a given system is an important challenge in nonlinear dynamics. This is especially true in the context of ventricular fibrillation in the heart, where the importance of the thickness of the ventricular wall is contentious. Here, we study defect-mediated turbulence arising in two different regimes in a conceptual model of excitable media and investigate how the statistical character of the turbulence changes if the thickness of the medium is changed from (quasi-) two- dimensional to three dimensional. We find that the thickness of the medium does not have a significant influence in, far from onset, fully developed turbulence while there is a clear transition if the system is close to a spiral instability. We provide clear evidence that the observed transition and change in the mechanism that drives the turbulent behavior is purely a consequence of the dimensionality of the medium. Using filament tracking, we further show that the statistical properties in the three-dimensional medium are different from those in turbulent regimes arising from filament instabilities like the negative line tension instability. Simulations also show that the presence of this unique three-dimensional turbulent dynamics is not model specific.

  4. 21 CFR 864.8500 - Lymphocyte separation medium.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Lymphocyte separation medium. 864.8500 Section 864.8500 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Reagents § 864.8500 Lymphocyte separation...

  5. Medium carbon vanadium steels for closed die forging

    International Nuclear Information System (INIS)

    Jeszensky, Gabor; Plaut, Ronald Lesley

    1993-01-01

    This work analyses the medium carbon micro alloyed vanadium potential for closed die forged production. The steels reach the mechanical resistance requests during cooling after forging, eliminating the subsequent thermal treatment. Those steels also present good fatigue resistance and machinability. The industrial scale experiments are also reported

  6. Towards a medium-range coastal station fog forecasting system

    CSIR Research Space (South Africa)

    Landman, S

    2013-09-01

    Full Text Available -1 29th Annual conference of South African Society for Atmospheric Sciences (SASAS) 2013 http://sasas.ukzn.ac.za/homepage.aspx Towards a Medium-Range Coastal Station Fog Forecasting System Stephanie Landman*1, Estelle Marx1, Willem A. Landman2...

  7. Ecological sanitation in urban medium density mixed housing

    CSIR Research Space (South Africa)

    Matsebe, G

    2011-07-01

    Full Text Available are unique to the use of Ecosan in urban Medium-Density Mixed-Housing (MDMH). Firstly, decision-making is more complex because consensus needs to be reached among a diverse group of people with a varied level of understanding of ecological and sustainable...

  8. New Conditions for a Total Neutrino Conversion in a Medium

    OpenAIRE

    Chizhov, M. V.; Petcov, S. T.

    1999-01-01

    A new effect of total neutrino conversion is possible when neutrino propagates through multi-layer medium of nonperiodic constant density layers. The effect can take place in the oscillations in the Earth of the Earth-core-crossing solar and atmospheric neutrinos.

  9. Development of a new medium containing date syrup for production ...

    African Journals Online (AJOL)

    Development of a new medium containing date syrup for production of bleomycin by Streptomyces mobaraensis ATCC 15003 using response surface methodology. Hesham H Radwan, Fars K Alanazi, Ehab I Taha, Hamdy A Dardir, Ihab M Moussa, Ibrahim A Alsarra ...

  10. the influence of cartoons as instructional medium on secondary ...

    African Journals Online (AJOL)

    Global Journal

    This study examined the influence of cartoon strips as instructional medium on the academic performance of secondary school students in Cross River State. The instrument used was a structured. Achievement Test in Fine Arts (SATFA). The sample used consisted of 46 Junior Secondary School two students. SATFA was ...

  11. Role of Cooperatives in Small and Medium Scale Enterprises (SMEs ...

    African Journals Online (AJOL)

    The paper examined the contributions of cooperatives to small and medium scale enterprises (SME) development, identified the challenges and to suggested ways of strengthening this role. The paper argued that SMEs are the key to Nigeria‟s prosperity, but that past efforts by the government to promote SMEs has not ...

  12. Effective-medium tight-binding model for silicon

    DEFF Research Database (Denmark)

    Stokbro, Kurt; Chetty, N.; Jacobsen, Karsten Wedel

    1994-01-01

    A method for calculating the total energy of Si systems, which is based on the effective-medium-theory concept of a reference system, is presented. Instead of calculating the energy of an atom in the system of interest, a reference system is introduced where the local surroundings are similar. Th...

  13. Potential mechanisms behind contrast medium-induced nephropathy

    African Journals Online (AJOL)

    How contrast medium-induced nephropathy (CIN) comes about is poorly understood, although CIN is a common cause of acute renal failure. Hitherto, the various studies performed have led to different interpretations and partially contradictory conclusions. This article aimed to review the mechanisms underlying CIN and to ...

  14. Small and Medium Enterprises, Job Creation, and Sustainability ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Small and Medium Enterprises, Job Creation, and Sustainability: Maximizing ... job creation, human capital, and green production and technologies will only materialize if ... IWRA/IDRC webinar on climate change and adaptive water management. International Water Resources Association, in close collaboration with IDRC, ...

  15. An improved stress corrosion test medium for aluminum alloys

    Science.gov (United States)

    Humphries, T. S.; Coston, J. E.

    1981-01-01

    A laboratory test method that is only mildly corrosive to aluminum and discriminating for use in classifying the stress corrosion cracking resistance of aluminum alloys is presented along with the method used in evaluating the media selected for testing. The proposed medium is easier to prepare and less expensive than substitute ocean water.

  16. Affordance of English-Medium Instruction Contexts in Taiwan

    Science.gov (United States)

    Huang, Yi-Ping; Jhuang, Wun-Ting

    2015-01-01

    The proliferation of English-medium instruction (EMI) in nonnative English-speaking (NNES) contexts has compelled researchers to explore the challenges students face in such environments. Mostly quantitative in nature with foci on language-related difficulties in one type of institution or curriculum, these studies obscure the complexity of NNES…

  17. A scheme for training effective English Second Language medium of ...

    African Journals Online (AJOL)

    Effective second language medium of instruction refers to an instructional approach that differs from that of regular, first language content instruction or a language across the curriculum approach. This approach uses language teaching strategies in subjects other than the formal language classes, to promote both ...

  18. Learning in an estimated medium-scale DSGE model

    Czech Academy of Sciences Publication Activity Database

    Slobodyan, Sergey; Wouters, R.

    2012-01-01

    Roč. 36, č. 1 (2012), s. 26-46 ISSN 0165-1889 R&D Projects: GA ČR(CZ) GCP402/11/J018 Institutional support: PRVOUK-P23 Keywords : constant-gain adaptive learning * medium-scale DSGE model * DSGE- VAR Subject RIV: AH - Economics Impact factor: 0.807, year: 2012

  19. Matrix albedo for discrete ordinates infinite-medium boundary condition

    International Nuclear Information System (INIS)

    Mathews, K.; Dishaw, J.

    2007-01-01

    Discrete ordinates problems with an infinite exterior medium (reflector) can be more efficiently computed by eliminating grid cells in the exterior medium and applying a matrix albedo boundary condition. The albedo matrix is a discretized bidirectional reflection distribution function (BRDF) that accounts for the angular quadrature set, spatial quadrature method, and spatial grid that would have been used to model a portion of the exterior medium. The method is exact in slab geometry, and could be used as an approximation in multiple dimensions or curvilinear coordinates. We present an adequate method for computing albedo matrices and demonstrate their use in verifying a discrete ordinates code in slab geometry by comparison with Ganapol's infinite medium semi-analytic TIEL benchmark. With sufficient resolution in the spatial and angular grids and iteration tolerance to yield solutions converged to 6 digits, the conventional (scalar) albedo boundary condition yielded 2-digit accuracy at the boundary, but the matrix albedo solution reproduced the benchmark scalar flux at the boundary to all 6 digits. (authors)

  20. The influence of cartoons as instructional medium on secondary ...

    African Journals Online (AJOL)

    This study examined the influence of cartoon strips as instructional medium on the academic performance of secondary school students in Cross River State. The instrument used was a structured Achievement Test in Fine Arts (SATFA). The sample used consisted of 46 Junior Secondary School two students. SATFA was ...