Division of Forestry Directory

Science.gov (United States)

Conservation Education Timber Management Wildland Fire & Aviation Burn Permits Firewise Alaska Brochure -8441 Safety Officer Tom Greiling thomas.greiling@alaska.gov (907) 761-6247 Fire Prevention Officer /SATS Radio Shop 5900 East Tudor Road Anchorage, AK 99507 nathan.skinner@alaska.gov (907) 451-2810 State

Una guía sobre las aves de Colombia

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Jorge E. Orejuela G.

1988-10-01

Full Text Available A guide to the birds of Colombia. Steven L. Hilty y William L. Browm. Ilustrador principal: Guy Tudor, con planchas de H. W. Trimm. J. Gwynne, J. Yrizarry y P. Prall. Dibujos de M. Kleinbaunm. Princenton University Press, Princenton (Nueva Jersey, 1986.

Right-Linear Languages Generated in Systems of Knowledge Representation based on LSG

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Daniela Danciulescu

2017-04-01

Full Text Available In Tudor (Preda (2010 a method for formal languages generation based on labeled stratified graph representations is sketched. The author proves that the considered method can generate regular languages and context-sensitive languages by considering an exemplification of the proposed method for a particular regular language and another one for a particular contextsensitive language. At the end of the study, the author highlights some open problems for future research among which we remind: (1 The study of the language families that can be generated by means of these structures; (2 The study of the infiniteness of the languages that can be represented in stratified graphs. In this paper, we extend the method presented in Tudor (Preda(2010, by considering the stratified graph formalism in a system of knowledge representation and reasoning. More precisely, we propose a method that can be applied for generating any Right Linear Language construction. Our method is proved and exemplified in several cases.

Genome-wide analysis of uncapped mRNAs under heat stress in Arabidopsis

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Emilio Gutierrez-Beltran

2015-09-01

Full Text Available Recently, we have showed that Tudor Staphylococcal Nuclease (TSN or Tudor-SN proteins (TSN1 and TSN2 are localized in cytoplasmic messenger ribonucleoprotein (mRNP complexes called stress granules (SG and processing bodies (PB under heat stress in Arabidopsis. One of the primary functions of these mRNP complexes is mRNA decay, which generates uncapped mRNAs by the action of endonucleases and decapping enzymes (Thomas et al., 2011 [1]. In order to figure out whether TSN proteins could be implicated in mRNA decay, we isolated uncapped and total mRNAs of Wild type (WT; Col and Ler and TSN double knock-out (tsn1tsn2 seedlings grown under heat stress (39 °C for 40 min and control (23 °C conditions. Here, we provide the experimental procedure to reproduce the results (NCBI GEO accession number GSE63522 published by Gutierrez-Beltran et al. (2015 in The Plant Cell [2].

Structural and Histone Binding Ability Characterizations of Human PWWP Domains

Energy Technology Data Exchange (ETDEWEB)

Wu, Hong; Zeng, Hong; Lam, Robert; Tempel, Wolfram; Amaya, Maria F.; Xu, Chao; Dombrovski, Ludmila; Qiu, Wei; Wang, Yanming; Min, Jinrong (Toronto); (Penn)

2013-09-25

The PWWP domain was first identified as a structural motif of 100-130 amino acids in the WHSC1 protein and predicted to be a protein-protein interaction domain. It belongs to the Tudor domain 'Royal Family', which consists of Tudor, chromodomain, MBT and PWWP domains. While Tudor, chromodomain and MBT domains have long been known to bind methylated histones, PWWP was shown to exhibit histone binding ability only until recently. The PWWP domain has been shown to be a DNA binding domain, but sequence analysis and previous structural studies show that the PWWP domain exhibits significant similarity to other 'Royal Family' members, implying that the PWWP domain has the potential to bind histones. In order to further explore the function of the PWWP domain, we used the protein family approach to determine the crystal structures of the PWWP domains from seven different human proteins. Our fluorescence polarization binding studies show that PWWP domains have weak histone binding ability, which is also confirmed by our NMR titration experiments. Furthermore, we determined the crystal structures of the BRPF1 PWWP domain in complex with H3K36me3, and HDGF2 PWWP domain in complex with H3K79me3 and H4K20me3. PWWP proteins constitute a new family of methyl lysine histone binders. The PWWP domain consists of three motifs: a canonical {beta}-barrel core, an insertion motif between the second and third {beta}-strands and a C-terminal {alpha}-helix bundle. Both the canonical {beta}-barrel core and the insertion motif are directly involved in histone binding. The PWWP domain has been previously shown to be a DNA binding domain. Therefore, the PWWP domain exhibits dual functions: binding both DNA and methyllysine histones.

Physical activity of female Malay Muslims before, during and after ...

African Journals Online (AJOL)

Ramadan is the fasting month for Muslims, during which physical activities like sports may be held in abeyance for a more spiritual life. ... on the standard by Tudor-Locke and Bassett (2004) in which a minimum of 10,000 steps a day denotes an 'active' lifestyle, deemed sufficient to confer health benefits to the individual.

SOUTH AFRICAN ARMY RANKS AND INSIGNIA

African Journals Online (AJOL)

the lieutenant-colonel a crown and one star, the major a crown, and the captain and the lieuten- ant respectively three and two stars. The crown was the "Tudor" or "king's" pattern and the star, popularly called a "pip", was based on the Order of the Bath. Warrant officers also wore a crown, those hold- ing senior positions.

What Kinds of play is Romeo and Juliet ? | Jeffery | Shakespeare in ...

African Journals Online (AJOL)

This story was well known in Shakespeare's day, and he uses it as cover for the third kind, which is covertly political, concerned with pressing issues arising from Tudor rule. With the passage of time awareness of the first and third kinds faded, so that the play has come to be understood simply as a wonderful sad love-story.

Cover changes and regeneration status of a peri-urban mangrove

OpenAIRE

Mohamed, M.O.S.; Neukermans, G.; Kairo, J.; Dahdouh-Guebas, F.; Koedam, N.

2008-01-01

Stability of an ecosystem is determined by its resilience, regenerative capacity and numerous weak trophic links, amongst other natural and human induced factors. The Tudor creek mangroves, a typical peri-urban mangrove, are exposed to both episodic natural and recurrent human disturbances, including decades? long exposure to raw domestic sewage, sporadic unregulated-harvesting and episodic siltation. This study evaluates the regeneration patterns within extended gaps and the understorey. An ...

Towards a balanced account of autism etiology

OpenAIRE

Hall, Genae A.

2004-01-01

Drash and Tudor describe six sets of reinforcement contingencies which may be present in the environments of some children eventually diagnosed with autism and suggest that these contingencies account for the etiology of “autistic” behaviors. Nevertheless, merely observing such contingencies in the environments of these children is insufficient to establish a positive correlation between the contingencies and “autistic” behaviors, let alone a causal relationship. To demonstrate a positive cor...

Hear After: Matters of Life and Death in David Tudor’s Electronic Music

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You Nakai

2014-09-01

Full Text Available In David Tudor’s electronic music, home-brew modular devices were carefully connected together to form complex feedback networks wherein all components—including the composer/performer himself—could only partially ‘influence’ one another. Once activated, the very instability of mismatched connections between the components triggered a cascade of signals and signal modulations, so that the work “composed itself,” and took “a life of its own.” Due to this self-producing, perpetuating nature of his works, Tudor insisted on what he called “the view from inside,” focusing more on the internal observation of his devices and sound than in materials external to the immanence of performance. When Tudor passed away in 1996, it became apparent that the sheer lack of resources outside the work—scores, instructions, recordings, texts—had made many of his music impossible to perform in his absence. The works that took a life of their own could not survive their composer’s death partially because of his utter reliance on them to do their work. By connecting often mismatched resources obtained from extended research on Tudor, this paper presents modular observations that seem to offer certain perspectives on the issue of life and death surrounding Tudor’s music. A comparison with developments in systems theory, most notably autopoiesis, outlines a mechanism for the endless life of sounds that compose themselves. Moving out of this theoretical reflection, a fieldwork report of an ongoing attempt to ‘revive’ some of Tudors works is offered. This report demonstrates the observer shifting from one ‘inside’ to another—from an electronic circuitry inside a particular device, to a network composed of several devices, and further into the activation of a composite instrument. Meandering away from the archives, the composer’s “view from inside” of his electronic devices is set side by side with recent insights of object

The multi-domain protein Np95 connects DNA methylation and histone modification.

Science.gov (United States)

Rottach, Andrea; Frauer, Carina; Pichler, Garwin; Bonapace, Ian Marc; Spada, Fabio; Leonhardt, Heinrich

2010-04-01

DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. Using in vitro binding assays we observed a weak preference of Np95 and its SRA (SET- and Ring-associated) domain for hemimethylated CpG sites. However, the binding kinetics of Np95 in living cells was not affected by the complete loss of genomic methylation. Investigating further links with heterochromatin, we could show that Np95 preferentially binds histone H3 N-terminal tails with trimethylated (H3K9me3) but not acetylated lysine 9 via a tandem Tudor domain. This domain contains three highly conserved aromatic amino acids that form an aromatic cage similar to the one binding H3K9me3 in the chromodomain of HP1ss. Mutations targeting the aromatic cage of the Np95 tandem Tudor domain (Y188A and Y191A) abolished specific H3 histone tail binding. These multiple interactions of the multi-domain protein Np95 with hemimethylated DNA and repressive histone marks as well as with DNA and histone methyltransferases integrate the two major epigenetic silencing pathways.

The answers are within me. An evaluation of a person centred counselling service for men at HMP Doncaster who have had experience of domestic violence 2005-2007

OpenAIRE

Hamilton, P.; Wilkinson, K.; Meadows, L.; Cadet, N.

2008-01-01

This report is the second year evaluation of the person centred counselling service for male victims and perpetrators of domestic violence at HMP Doncaster in 2006/7. This report follows on from the previous year's evaluation, Raging Anger Within Me, which evaluated this project in 2005/6. Initiated by the Doncaster Rape and Sexual Abuse Counselling Centre (DRSACC), the second year of counselling service delivery was also funded by Lloyds TSB and the Tudor Trust.\\ud The report provides a back...

Women who served Queen Mary and Queen Elizabeth : Ladies, Gentlewomen and Maids of the Privy Chamber, 1553-1603.

OpenAIRE

Merton, Charlotte Isabelle.

1992-01-01

A brief introduction to the history of the Tudor Court and the subject in hand is enlarged upon in the second 'chapter ('The Court'). which describes the nature of the evidence available, the process of admission to the Privy Chamber, the hierarchy within it and the titles given to the various Privy Chamber posts, and official payment (wages, food and accommodation). Chamber reform and the status of the womens' own servants are also discussed. The third chapter ('Recruitment') turns to the cr...

Collections XVII (The Malone Society)

OpenAIRE

Keenan, Siobhan; Giddens, Eugene

2016-01-01

Collections XVII is the latest volume in the Malone Society's pioneering series of editions of miscellaneous documents relating to English theatre and drama before 1642. It is likely to be of special interest not only to early theatre historians but to those working on Tudor and Stuart court and civic culture, manuscript writing, household drama and early modern women's writing, as it publishes new material in each of these fields. The book includes items such as Revels Office accounts, a pla...

Pride and penitence in some Tudor histories of Thomas Wolsey

African Journals Online (AJOL)

2016-05-26

May 26, 2016 ... Limitations of space have made it necessary to exclude many texts, including the poems of John Skelton; Polydore Vergil's Anglica historia; polemics by Robert. Barnes, Simon Fish, and William Tyndale; Samuel Rowley's play When You See Me,. You Know Me; the collection of poems Mirror for Magistrates ...

Review Essay: Does Qualitative Network Analysis Exist?

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Rainer Diaz-Bone

2007-01-01

Full Text Available Social network analysis was formed and established in the 1970s as a way of analyzing systems of social relations. In this review the theoretical-methodological standpoint of social network analysis ("structural analysis" is introduced and the different forms of social network analysis are presented. Structural analysis argues that social actors and social relations are embedded in social networks, meaning that action and perception of actors as well as the performance of social relations are influenced by the network structure. Since the 1990s structural analysis has integrated concepts such as agency, discourse and symbolic orientation and in this way structural analysis has opened itself. Since then there has been increasing use of qualitative methods in network analysis. They are used to include the perspective of the analyzed actors, to explore networks, and to understand network dynamics. In the reviewed book, edited by Betina HOLLSTEIN and Florian STRAUS, the twenty predominantly empirically orientated contributions demonstrate the possibilities of combining quantitative and qualitative methods in network analyses in different research fields. In this review we examine how the contributions succeed in applying and developing the structural analysis perspective, and the self-positioning of "qualitative network analysis" is evaluated. URN: urn:nbn:de:0114-fqs0701287

Edition and Traslation of the «Carta a lord Burghley» by Pedro Sarmiento de Gamboa

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Andrés Eichmann Oehrli

2016-05-01

Full Text Available In the following article we present an unpublished and unknown text of Galician discoverer Pedro Sarmiento de Gamboa. The letter, wrote in Latin, was send to Lord Burghley, the first minister of Elizabeth Tudor. Its content links chro-nicles and legends of Spain and Galicia to the genealogy of Sarmiento de Gamboa. Here, we offer the biographical context of the letter, a brief study of the sources, apart from the transcription of the original and the translation of the text into Spanish.

The multi-domain protein Np95 connects DNA methylation and histone modification

Science.gov (United States)

Rottach, Andrea; Frauer, Carina; Pichler, Garwin; Bonapace, Ian Marc; Spada, Fabio; Leonhardt, Heinrich

2010-01-01

DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. Using in vitro binding assays we observed a weak preference of Np95 and its SRA (SET- and Ring-associated) domain for hemimethylated CpG sites. However, the binding kinetics of Np95 in living cells was not affected by the complete loss of genomic methylation. Investigating further links with heterochromatin, we could show that Np95 preferentially binds histone H3 N-terminal tails with trimethylated (H3K9me3) but not acetylated lysine 9 via a tandem Tudor domain. This domain contains three highly conserved aromatic amino acids that form an aromatic cage similar to the one binding H3K9me3 in the chromodomain of HP1ß. Mutations targeting the aromatic cage of the Np95 tandem Tudor domain (Y188A and Y191A) abolished specific H3 histone tail binding. These multiple interactions of the multi-domain protein Np95 with hemimethylated DNA and repressive histone marks as well as with DNA and histone methyltransferases integrate the two major epigenetic silencing pathways. PMID:20026581

Regulation of adipogenesis by nucelar receptor PPARγ is modulated by the histone demethylase JMJD2C

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Lizcano Fernando

2011-01-01

Full Text Available A potential strategy to combat obesity and its associated complications involves modifying gene expression in adipose cells to reduce lipid accumulation. The nuclear receptor Peroxisome Proliferator-activated receptor gamma (PPARγ is the master regulator of adipose cell differentiation and its functional activation is currently used as a therapeutic approach for Diabetes Mellitus type 2. However, total activation of PPARγ induces undesirable secondary effects that might be set with a partial activation. A group of proteins that produce histone demethylation has been shown to modify the transcriptional activity of nuclear receptors. Here we describe the repressive action of the jumonji domain containing 2C/lysine demethylase 4 C (JMJD2C/KDM4C on PPARγ transcriptional activation. JMJD2C significantly reduced the rosiglitazone stimulated PPARγ activation. This effect was mainly observed in experiments performed using the Tudor domains that may interact with histone deacetylase class 1 (HDAC and this interaction probably reduces the mediated activation of PPARγ. Trichostatin A, a HDAC inhibitor, reduces the repressive effect of JMJD2C. When JMJD2C was over-expressed in 3T3-L1 cells, a reduction of differentiation was observed with the Tudor domain. In summary, we herein describe JMJD2C-mediated reduction of PPARgamma transcriptional activation as well as preadipocyte differentiation. This novel action of JMJD2C might have an important role in new therapeutic approaches to treat obesity and its complications.

Usual physical activity in children and adolescents measured by pedometer and its association with nutritional indicators.DOI: 10.5007/1980-0037.2011v13n1p22

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Henrique Luiz Monteiro

2011-01-01

Full Text Available Monitoring physical activity levels in segments of the young population has become an important topic among specialists. The objective of this study was to analyze the physical activity level of schoolchildren based on two current recommendations and to examine the association between the number of steps/day and different indicators of adiposity. The sample consisted of 162 subjects aged 10 to 18 years (65 boys and 97 girls. Body mass index and body fat percentage were calculated. The level of physical activity was quantified with a pedometer (New Lifestyles NL-2000 based on the following recommendations: Duncan et al. (male: 16,000 steps/day and female: 13,000 steps/day and Tudor-Locke et al. (male: 15,000 steps/day and female: 12,000 steps/day. The Student t-test and one-way ANOVA (LSD post hoc test were used for comparison between groups. A level of significance of 5% was adopted (p £ 0.05. Only 18.5% of the sample met the recommendations proposed by Duncan et al. and 25.9% met the cutoff proposed by Tudor-Locke et al. Adolescents who did not meet the cutoff proposed by Duncan had higher body fat percentages than those who did (p < 0.05. In conclusion, a high rate of the youngsters did not meet the recommendations analyzed and only one indicator of adiposity was associated with meeting one of the recommendations.

The Medieval Dublin Project: A Case Study

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Niall O'hOisin

2010-04-01

Full Text Available This paper provides an overview of the Medieval Dublin Project. It covers the development and release of the DVD ‘Medieval Dublin: From Vikings to Tudors (Schools Edition,’ and outlines the major virtual and interactive features developed for that release. The paper also covers the collaboration that took place between the DVD development team and the academic community and discusses the ways in which 3D visualisations, timelines, interactivity and character-based storytelling were used to present Dublin’s archaeological heritage in an engaging and interesting way

Definition of domain boundaries and crystallization of the SMN Tudor domain

NARCIS (Netherlands)

Sprangers, Remco; Selenko, Philipp; Sattler, Michael; Sinning, Irmgard; Groves, Matthew R

Spinal muscular atropy (SMA) is the major genetic disease leading to childhood mortality and is caused by mutations in or deletions of the smn1 gene. The human survival of motor neurons (SMN) protein encoded by this gene plays an important role in the assembly of snRNPs (small nuclear

TESIS DOCTORALES Doctoral dissertations

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Esteban Hernández Esteve

2006-07-01

Full Text Available TESIS DOCTORALES Doctoral dissertations María Soledad Campos Lucena: El control de las arcas municipales a través de la rendición de cuentas. La transformación del proceso del Antiguo al Nuevo régimen y la consolidación del modelo liberal: 1745-1914 The control of municipal coffers by means of account rendering. The change from Ancien Régime to the New Regime and the consolidation of liberalism: 1745-1914 Candelaria Castro Pérez: La institución parroquial a través de los registros contables del Señorío episcopal de la Villa de Agüimes. (1500-1860 The parochial institution seen through the account books of the Episcopal domain of the city of Aguimes (1500-1860 José Julián Hernández Borreguero: El Cabildo Catedral de Sevilla: organización y sistema contable. (1625-1650 Administrative and accounting organization of the Seville Cathedral. (1625-1650 Juan Lanero Fernández: El esplendor de la teneduría de libros: la partida doble en los tratados contables ingleses de la dinastia Tudor (1543-1588 Bookkeeping splendor: double-entry in the English accounting treatises at the time of the Tudor dynasty (1543-1588 María Llompart Bibiloni: Un análisis histórico-contable de la Procuración del Real Patrimonio en el Reino de Mallorca, período 1310-1330 An accounting historical análisis of the Royal Exchequer of the Kingdom of Mallorca (1310-1330

UNDERGROUND ECONOMY, GDP AND STOCK MARKET

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Caus Vasile Aurel

2012-07-01

Full Text Available Economic growth is affected by the size and dynamics of underground economy. Determining this size is a subject of research for many authors. In this paper we present the relationship between underground economy dynamics and the dynamics of stock markets. The observations are based on regression used by Tanzi (1983 and the relationship between GDP and stock market presented in Tudor (2008. The conclusion of this paper is that the dynamics of underground economy is influenced by dynamic of financial markets. Thus, using specific stock market mathematical tools analysis, one can analyze the dynamic of underground economy

The globalization of naval provisioning: ancient DNA and stable isotope analyses of stored cod from the wreck of the Mary Rose, AD 1545.

Science.gov (United States)

Hutchinson, William F; Culling, Mark; Orton, David C; Hänfling, Bernd; Lawson Handley, Lori; Hamilton-Dyer, Sheila; O'Connell, Tamsin C; Richards, Michael P; Barrett, James H

2015-09-01

A comparison of ancient DNA (single-nucleotide polymorphisms) and carbon and nitrogen stable isotope evidence suggests that stored cod provisions recovered from the wreck of the Tudor warship Mary Rose, which sank in the Solent, southern England, in 1545, had been caught in northern and transatlantic waters such as the northern North Sea and the fishing grounds of Iceland and Newfoundland. This discovery, underpinned by control data from archaeological samples of cod bones from potential source regions, illuminates the role of naval provisioning in the early development of extensive sea fisheries, with their long-term economic and ecological impacts.

The globalization of naval provisioning: ancient DNA and stable isotope analyses of stored cod from the wreck of the Mary Rose, AD 1545

Science.gov (United States)

Hutchinson, William F.; Culling, Mark; Orton, David C.; Hänfling, Bernd; Lawson Handley, Lori; Hamilton-Dyer, Sheila; O'Connell, Tamsin C.; Richards, Michael P.; Barrett, James H.

2015-01-01

A comparison of ancient DNA (single-nucleotide polymorphisms) and carbon and nitrogen stable isotope evidence suggests that stored cod provisions recovered from the wreck of the Tudor warship Mary Rose, which sank in the Solent, southern England, in 1545, had been caught in northern and transatlantic waters such as the northern North Sea and the fishing grounds of Iceland and Newfoundland. This discovery, underpinned by control data from archaeological samples of cod bones from potential source regions, illuminates the role of naval provisioning in the early development of extensive sea fisheries, with their long-term economic and ecological impacts. PMID:26473047

The Demethylase JMJD2C Localizes to H3K4me3 Positive Transcription Start Sites and Is Dispensable for Embryonic Development

DEFF Research Database (Denmark)

Pedersen, Marianne Terndrup; Agger, Karl; Laugesen, Anne

2014-01-01

cell (ESC) self-renewal and embryonic development. Moreover, we report that JMJD2C localizes to H3K4me3 positive transcription start sites in both primary cells and in the human carcinoma KYSE150 cell line, containing an amplification of the JMJD2C locus. Binding is dependent on the double Tudor domain...... expression of a subset of target genes involved in cell cycle progression. Taken together, we show that JMJD2C is targeted to H3K4me3 positive transcription start sites, where it can contribute to transcriptional regulation, and report that the putative oncogene, JMJD2C, is not generally required...

Historical figures at the office of Endocrinology.

Science.gov (United States)

Alfaro-Martínez, José J

2014-01-01

Some historical figures have suffered endocrine diseases. This review relates those whose diseases have been published in the scientific literature. It takes a biographical summary and describes the disease process in those considered most relevant by the nature of the disease or the importance of the figure in the Spanish and Latin American context: the Pharaoh Akhenaten, Maximinus I, Bodhidharma, Sancho I of Leon, William the Conqueror, Enrique IV of Castile, Henry VIII, Mary Tudor, Carlos II of Spain, Pio Pico, Pedro II of Brazil, Eisenhower and J. F. Kennedy. Copyright © 2013 SEEN. Published by Elsevier Espana. All rights reserved.

Requirement for the Phospho-H2AX Binding Module of Crb2 in Double-Strand Break Targeting and Checkpoint Activation▿

Science.gov (United States)

Sanders, Steven L.; Arida, Ahmad R.; Phan, Funita P.

2010-01-01

Activation of DNA damage checkpoints requires the rapid accumulation of numerous factors to sites of genomic lesions, and deciphering the mechanisms of this targeting is central to our understanding of DNA damage response. Histone modification has recently emerged as a critical element for the correct localization of damage response proteins, and one key player in this context is the fission yeast checkpoint mediator Crb2. Accumulation of Crb2 at ionizing irradiation-induced double-strand breaks (DSBs) requires two distinct histone marks, dimethylated H4 lysine 20 (H4K20me2) and phosphorylated H2AX (pH2AX). A tandem tudor motif in Crb2 directly binds H4K20me2, and this interaction is required for DSB targeting and checkpoint activation. Similarly, pH2AX is required for Crb2 localization to DSBs and checkpoint control. Crb2 can directly bind pH2AX through a pair of C-terminal BRCT repeats, but the functional significance of this binding has been unclear. Here we demonstrate that loss of its pH2AX-binding activity severely impairs the ability of Crb2 to accumulate at ionizing irradiation-induced DSBs, compromises checkpoint signaling, and disrupts checkpoint-mediated cell cycle arrest. These impairments are similar to that reported for abolition of pH2AX or mutation of the H4K20me2-binding tudor motif of Crb2. Intriguingly, a combined ablation of its two histone modification binding modules yields a strikingly additive reduction in Crb2 activity. These observations argue that binding of the Crb2 BRCT repeats to pH2AX is critical for checkpoint activity and provide new insight into the mechanisms of chromatin-mediated genome stability. PMID:20679488

Requirement for the phospho-H2AX binding module of Crb2 in double-strand break targeting and checkpoint activation.

Science.gov (United States)

Sanders, Steven L; Arida, Ahmad R; Phan, Funita P

2010-10-01

Activation of DNA damage checkpoints requires the rapid accumulation of numerous factors to sites of genomic lesions, and deciphering the mechanisms of this targeting is central to our understanding of DNA damage response. Histone modification has recently emerged as a critical element for the correct localization of damage response proteins, and one key player in this context is the fission yeast checkpoint mediator Crb2. Accumulation of Crb2 at ionizing irradiation-induced double-strand breaks (DSBs) requires two distinct histone marks, dimethylated H4 lysine 20 (H4K20me2) and phosphorylated H2AX (pH2AX). A tandem tudor motif in Crb2 directly binds H4K20me2, and this interaction is required for DSB targeting and checkpoint activation. Similarly, pH2AX is required for Crb2 localization to DSBs and checkpoint control. Crb2 can directly bind pH2AX through a pair of C-terminal BRCT repeats, but the functional significance of this binding has been unclear. Here we demonstrate that loss of its pH2AX-binding activity severely impairs the ability of Crb2 to accumulate at ionizing irradiation-induced DSBs, compromises checkpoint signaling, and disrupts checkpoint-mediated cell cycle arrest. These impairments are similar to that reported for abolition of pH2AX or mutation of the H4K20me2-binding tudor motif of Crb2. Intriguingly, a combined ablation of its two histone modification binding modules yields a strikingly additive reduction in Crb2 activity. These observations argue that binding of the Crb2 BRCT repeats to pH2AX is critical for checkpoint activity and provide new insight into the mechanisms of chromatin-mediated genome stability.

Los usos políticos del cuerpo: los dos cuerpos del rey en la filosofía política de Francis Bacon

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Silvia Manzo

2008-01-01

Full Text Available Como figure destacada en el complejo escenario político de la transición entre la dinastía Tudor y la dinastía Estuardo de Inglaterra, Francis Bacon (1561-1626 adoptó la teoría de los dos cuerpos del rey en sus textos políticos y jurídicos. Su uso de esta teoría tiene una estrecha conexión con conceptos centrales de su filosofía natural que testimonia la profunda conexión que Bacon encuentra entre la política y la naturaleza. En este trabajo, analizaré su utilización de la teoría medieval de los dos cuerpos del rey y la vinculación que esta tiene con el resto de la filosofía baconiana. Tomaré como eje para el análisis su discurso sobre la naturalización de los escoceses nacidos después de la subida al trono de James I, donde Bacon trata el concepto de los dos cuerpos del rey con más profundidad.As a prominent intellectual figure in the complex political arena of England, during the transition from Tudor to Stuart dinasties, Francis Bacon (1561-1626 assumes the medieval King's two bodies theory in his political and juridical writings. His use of this theory is extremely linked to central concepts of his natural philosphy, which testifies to the deep connection that Bacon finds between politics and nature. This paper addresses Bacon's use of the King's two bodies theory and its link to the rest of the Baconian philosophy. The focus of this study will be his discourse on the naturalization of Scots born after James I assumption of power, where Bacon deals more deeply with the King's two bodies theory.

Британские истоки архитектурных форм дворца Бржозовского в Одессе

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Yuri Pismak

2016-10-01

Full Text Available An outstanding British architect Edward Blore (1787–1879 is the main author of project of Vorontsov’s Palace in Alupka (the Crimea, 1830–1848. In the opining of many researchers the Vorontsov’s Palace in Alupka is the best of Edward Blore’s works. Seemingly under impression of Vorontsov’s Alupka Palace, Brzhozovsky’s Palace (Shakh’s Palace. 1851–1852 was built to the project of a renowned Odessa architect Felix Gonsiorovski (1815–1891 in 2 Nadezhdinskaya (Gogol street in Odessa. Apart from Tudor elements and Castel style, these two Palaces are related yet by the fact that they both are situated on the Black Sea coast. Facades of Brzhozovsky’s Palace remind the same of a distinguished monument of architecture, historically connected with the names of British monarchs – the Hampton Court Palace. Hampton Court Palace was recognized as the acme of the English palace architecture. Splendid ensemble sprawling on the bank of the Thames River near London was initiated in 1514. The great vogue for all Gothic sprang up in St. Petersburg under the spell of Walter Scott’s (1771–1832 historic novels. The article tells also about prototypes of Tudor arch – an element which characterizes English architecture of 16th century. This form of the arch was spread to many countries from the United Kingdom. However, long before England, this form of arch was used by architects of Iran and other countries of Central Asia. Formation of Odessa’s and all Southern Region of the Ukraine architectural appearance in the XIXth century was influenced by different architectural European schools. Especially in this article is noted British influence. Consequently it seems proper to consider this process in the retrospect of the European development as a whole.

ŞTEFANIA CRISTESCU-GOLOPENŢIA’S CHRONOLOGY

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Professor Emerita Sanda Golopentia

2013-05-01

Full Text Available The present Chronology brings together data from the personal archive of Şt. C.-G. with data accumulated during the editing of her published and posthumous work that Sanda Golopenţia undertook after 1989. The author specifically calls attention to the new data regarding Şt. C.-G.’s 1938 and 1939 regional research in Ţara Oltului (the Olt County, the research she conducted in 1945 and 1947 in the Gurghiu Valley, and the social inquiry on the daily lives of her students in the Industrial Colege “Protopopul Tudor Economul” from Bucharest (1942–1943. The bibliography of Şt. C.-G.’s sociological, pedagogical and literary works was updated

Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage

DEFF Research Database (Denmark)

Gibbs-Seymour, Ian; Markiewicz, Ewa; Bekker-Jensen, Simon

2015-01-01

Lamins A/C have been implicated in DNA damage response pathways. We show that the DNA repair protein 53BP1 is a lamin A/C binding protein. In undamaged human dermal fibroblasts (HDF), 53BP1 is a nucleoskeleton protein. 53BP1 binds to lamins A/C via its Tudor domain, and this is abrogated by DNA...... damage. Lamins A/C regulate 53BP1 levels and consequently lamin A/C-null HDF display a 53BP1 null-like phenotype. Our data favour a model in which lamins A/C maintain a nucleoplasmic pool of 53BP1 in order to facilitate its rapid recruitment to sites of DNA damage and could explain why an absence...

Tailoring ISO/IEC 27001 for SMEs: A Guide to Implement an Information Security Management System in Small Settings

Science.gov (United States)

Valdevit, Thierry; Mayer, Nicolas; Barafort, Béatrix

While Information Security Management Systems (ISMS) are being adopted by the biggest IT companies, it remains quite difficult for smaller entities to implement and maintain all the requirements of ISO/IEC 27001. In order to increase information security in Luxembourg, the Public Research Centre Henri Tudor has been charged by the Luxembourg Ministry of Economy and Foreign Trade to find solutions to facilitate ISMS deployment for SMEs. After an initial experiment aiming at assisting a SME in getting the first national ISO/IEC 27001 certification for a private company, an implementation guide for deploying an ISMS, validated by local experts and experimented in SMEs, has been released and is presented in this paper.

IAEA statement on Iranian enrichment announcement

International Nuclear Information System (INIS)

2010-01-01

Full text: The following is a statement attributable to IAEA Spokesperson Gill Tudor: 'The IAEA can confirm that it has received a letter from the Atomic Energy Organization of Iran (AEOI) on 8 February 2010, in which the AEOI informed the Agency that production of less than 20% enriched uranium is being foreseen at the Pilot Fuel Enrichment Plant at Natanz for fuel for the Tehran Research Reactor'. 'IAEA Director General Yukiya Amano noted with concern this decision, as it may affect, in particular, ongoing international efforts to ensure the availability of nuclear fuel for the Tehran Research Reactor.' 'The Director General reiterated the Agency's readiness to play an intermediary role on the issue of the Tehran Research Reactor.' (IAEA)

PAR EXEMPLE... LA CITATION

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Maria Anca FILIP

2012-05-01

Full Text Available The aim of this paper is to discuss the relationship between the notion of “example” – as it is presented in the French dictionaries, and the manner in which it may be interpreted from the theoretical perspective suggested by Aristotle and Perelman & Olbrechts-Tyteca, respectively. We will also suggest a possible expression of the “example” in the form of “quotation”, and will support this view with evidence found in our corpus of data represented by the political discourses delivered by C. Vadim Tudor, J.- M. Le Pen and/or J. Haider, approximately in the same period. This perspective would expand the rhetorical interpretation of the “example”, including the “quotational” reading as well.

Catholic, Anglican, and Puritan Representations of Royal Martyrs

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Nick K Crown

2017-06-01

Full Text Available Abstract This journal article is derived from my doctoral thesis undertaken at UEA Norwich, which provided the first in–depth comparison of printed representations of Catholic and Protestant martyrdom in Tudor England since the work of McGrath and Dickens during the 1960s. In this piece of research, a martyr is defined as one who bore witness to persecution during the Tudor Reformation (c.1530-1600, and who ultimately died for his or her beliefs rather than abjure. The main themes discussed were issues of continuity and change: to what extent did Protestant depictions of martyrs draw upon pre–Reformation ideas? Were they a radical break from the past; or did they represent gradual evolution and transition in which some older beliefs were perpetuated, some were reinterpreted allegorically, and others were abandoned and replaced with new representations? Novel contributions to the historiography include the representation of non–martyrs (individuals executed for their religion but who failed to gain full recognition in Catholic or Protestant martyrologies; Puritan efforts to reform the Church of England internally by supplanting lingering pre–Reformation rituals, relics and images with abstract, Old Testament inspired sermons; and the depiction of persecutors’ untimely deaths as evidence not only of divine providence, but also of the illegitimacy of rival churches. Additionally, I have examined depictions of the state’s dominance over the criminal’s body and the extent order was maintained through terror or, conversely, willing popular consent. Although firmly grounded in history, my methodology also incorporated elements from other disciplines, especially gender studies, death studies, religion, philosophy, and some aspects of art history. In particular, I have reassessed gender roles in the sixteenth century, and discussed the language of inversion, where exceptionally courageous female martyrs were portrayed with the masculine

Gelo nos Trópicos: a exportação de “Blocos de cristais da frieza ianque” para Índia e Brasil

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Marc W. Herold

2011-10-01

Full Text Available O comércio de gelo natural de Boston prosperou durante 1830-70 com base na idéia de Frederic Tudor da combinação de dois produtos inúteis – gelo natural do inverno das lagoas de Nova Inglaterra e serragem de madeireiras de Maine. O gelo Tudor foi exportado extensamente para os trópicos, desde as Índias Ocidentais até o Brasil e as Índias Orientais, assim como para os portos do sul dos Estados Unidos. Nos portos tropicais de gelo, o gelo natural importado era um produto de luxo, por exemplo, sendo servido para esfriar vinhos claret (Calcutá, champanha (Havana e Manaus, e juleps de hortelã (Nova Orleans e Savannah e utilizado também em hotéis de luxo ou em banquetes. No clima temperado dos Estados Unidos, o gelo natural era usado para preservar alimentos (frigoríficos e para esfriar a água (o amor peculiar dos americanos por água gelada. Nas regiões temperadas e tropicais, o gelo natural foi usado para fins medicinais (para acalmar as febres. Com a invenção de uma nova tecnologia para a fabricação de gelo artificial como parte da Revolução Industrial, o comércio de exportação de gelo natural diminuiu assim como a industrialização por substituição de importações acontecia nos trópicos. Na virada do século XX, fábricas de gelo tinham sido estabelecidas em meia dúzia de cidades portuárias brasileiras. Tudo o que restava do que fosse um comércio extenso e global de gelo natural era um veleiro que aportou no Rio de Janeiro na época de Natal cheio de gelo e maçãs da Nova Inglaterra.

Expression of germline markers in three species of amphioxus supports a preformation mechanism of germ cell development in cephalochordates

Science.gov (United States)

2013-01-01

Background In a previous study, we showed that the cephalochordate amphioxus Branchiostoma floridae has localized maternal transcripts of conserved germ cell markers Vasa and Nanos in its early embryos. These results provided strong evidence to support a preformation mechanism for primordial germ cell (PGC) development in B. floridae. Results In this study, we further characterize the expression of B. floridae homologs of Piwi and Tudor, which play important roles in germline development in diverse metazoan animals. We show that maternal mRNA of one of the identified Piwi-like homologs, Bf-Piwil1, also colocalizes with Vasa in the vegetal germ plasm and has zygotic expression in both the putative PGCs and the tail bud, suggesting it may function in both germline and somatic stem cells. More interestingly, one Tudor family gene, Bf-Tdrd7, is only expressed maternally and colocalizes with Vasa in germ plasm, suggesting that it may function exclusively in germ cell specification. To evaluate the conservation of the preformation mechanism among amphioxus species, we further analyze Vasa, Nanos, Piwil1, and Tdrd7 expression in two Asian amphioxus species, B. belcheri and B. japonicum. Their maternal transcripts all localize in similar patterns to those seen in B. floridae. In addition, we labeled putative PGCs with Vasa antibody to trace their dynamic distribution in developing larvae. Conclusions We identify additional germ plasm components in amphioxus and demonstrate the molecular distinction between the putative germline stem cells and somatic stem cells. Moreover, our results suggest that preformation may be a conserved mechanism for PGC specification among Branchiostoma species. Our Vasa antibody staining results suggest that after the late neurula stage, amphioxus PGCs probably proliferate with the tail bud cells during posterior elongation and are deposited near the forming myomere boundaries. Subsequently, these PGCs would concentrate at the ventral tip of the

The Geometry Conference

CERN Document Server

Bárány, Imre; Vilcu, Costin

2016-01-01

This volume presents easy-to-understand yet surprising properties obtained using topological, geometric and graph theoretic tools in the areas covered by the Geometry Conference that took place in Mulhouse, France from September 7–11, 2014 in honour of Tudor Zamfirescu on the occasion of his 70th anniversary. The contributions address subjects in convexity and discrete geometry, in distance geometry or with geometrical flavor in combinatorics, graph theory or non-linear analysis. Written by top experts, these papers highlight the close connections between these fields, as well as ties to other domains of geometry and their reciprocal influence. They offer an overview on recent developments in geometry and its border with discrete mathematics, and provide answers to several open questions. The volume addresses a large audience in mathematics, including researchers and graduate students interested in geometry and geometrical problems.

introduction Introdução/Indroduction

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Margarida Gandara Rauen

2008-04-01

Full Text Available Emphasis on William Shakespeare (1564-1616 as an outstanding poet and playwright has often led to oversimplified notions of English literature and drama during the late sixteenth and early seventeenth centuries, when many other remarkable writers existed. This period is generally called the Renaissance, despite the limitations of this term. Hollander and Kermode, for example, note that “By the English Renaissance,historians of literature and culture mean the period from about 1509 to 1660, the reign of the Tudor Henry VIII and his children and the first two Stuarts, and the revolutionary government of the Commonwealth...” (5 The articles in this issue of Ilha do Desterro cover the Elizabethan and Jacobean periods. They address the work of six major writers: the playwrights Christopher Marlowe (1564-1593, John Fletcher (1579-1625, Francis Beaumont (1584?-1616 and Ben Jonson (1572-1637; the poets John Donne (1572-1631 and George Herbert (1593-1633.

The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation.

Science.gov (United States)

Claycomb, Julie M; Batista, Pedro J; Pang, Ka Ming; Gu, Weifeng; Vasale, Jessica J; van Wolfswinkel, Josien C; Chaves, Daniel A; Shirayama, Masaki; Mitani, Shohei; Ketting, René F; Conte, Darryl; Mello, Craig C

2009-10-02

RNAi-related pathways regulate diverse processes, from developmental timing to transposon silencing. Here, we show that in C. elegans the Argonaute CSR-1, the RNA-dependent RNA polymerase EGO-1, the Dicer-related helicase DRH-3, and the Tudor-domain protein EKL-1 localize to chromosomes and are required for proper chromosome segregation. In the absence of these factors chromosomes fail to align at the metaphase plate and kinetochores do not orient to opposing spindle poles. Surprisingly, the CSR-1-interacting small RNAs (22G-RNAs) are antisense to thousands of germline-expressed protein-coding genes. Nematodes assemble holocentric chromosomes in which continuous kinetochores must span the expressed domains of the genome. We show that CSR-1 interacts with chromatin at target loci but does not downregulate target mRNA or protein levels. Instead, our findings support a model in which CSR-1 complexes target protein-coding domains to promote their proper organization within the holocentric chromosomes of C. elegans.

Meniscal transplantation: procedures, outcomes, and rehabilitation

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Young J

2017-05-01

Full Text Available James Young, Francois Tudor, Ahmed Mahmoud, Peter Myers Brisbane Orthopaedic & Sports Medicine Centre, Brisbane Private Hospital, Spring Hill, Brisbane, Queensland, Australia Abstract: Meniscal allograft transplantation (MAT is a possible treatment option for patients with joint pain after meniscectomy. It is necessary that the joint be aligned and stable. Current evidence shows that MAT improves pain and mechanical function in the mid to long term with patients reporting significantly improved outcomes at up to 15 years following surgery. Studies on survivorship showed up to 76% graft survival at 10 years. Recent studies have suggested a chondroprotective effect, but there is, at present, no evidence to support MAT in the prevention of osteoarthritis. This review article reported the current evidence for MAT showing support for fresh frozen, nonirradiated allografts. However, further research is required to determine the ideal indications for MAT, the optimal graft fixation method, and the safest rehabilitation protocol. Keywords: meniscus, meniscectomy, meniscal allograft transplantation

Mw Spectroscopy Coupled with Ultrafast UV Laser Vaporization: {RIBOSE} Found in the Gas Phase

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Cocinero, Emilio J.; Ecija, Patricia; Basterretxea, Francisco J.; Fernandez, Jose A.; Castano, Fernando; Lesarri, Alberto; Grabow, Jens-Uwe

2012-06-01

Sugars are aldoses or ketoses with multiple hydroxy groups which have been elusive to spectroscopic studies. Here we report a rotational study of the aldopentose ribose. According to any standard textbook aldopentoses can exhibit either linear forms, cyclic five-membered (furanose) structures or six-membered (pyranose) rings, occurring either as α- or β- anomers depending on the orientation of the hydroxy group at C-1 (anomeric carbon). β-Furanose is predominant in ribonucleosides, RNA, ATP and other biochemically relevant derivatives, but is β-furanose the native form also of free ribose? Recent condensed-phase X-ray and older NMR studies delivered conflicting results. In order to solve this question we conducted a microwave study on D-ribose that, owing to ultrafast UV laser vaporization, has become the first C-5 sugar observed with rotational resolution. The spectrum revealed six conformations of free ribose, preferentially adopting β-pyranose chairs as well as higher-energy α-pyranose forms. The method also allowed for unambiguous distinction between different orientations of the hydroxy groups, which stabilize the structures by cooperative hydrogen-bond networks. No evidence was observed of the α-/β-furanoses or linear forms found in the biochemical derivatives. i) D. Šišak, L. B. McCusker, G. Zandomeneghi, B. H. Meier, D. Bläser, R. Boese, W. B. Schweizer, R. Gylmour and J. D. Dunitz Angew. Chem. Int. Ed. 49, 4503, 2010. ii) W. Saenger Angew. Chem. Int. Ed. 49, 6487, 2010. i) M. Rudrum, and D. F. Shaw, J. Chem. Soc. 52, 1965. ii) R. U. Lemieux and J. D. Stevens Can. J. Chem. 44, 249, 1966. iii) E. Breitmaier and U. Hollstein Org. Magn. Reson. 8, 573, 1976. E. J. Cocinero, A. Lesarri, P. Écija, F. J. Basterretxea, J. U. Grabow, J. A. Fernández and F. Castaño Angew. Chem. Int. Ed. in press: DOI: 10.1002/anie.201107973, 2012.

Edinburgh and its role in the foundation of Sydney Medical School.

Science.gov (United States)

Walker-Smith, J

2006-12-01

In 1882, Thomas Anderson Stuart (1856-1920) was appointed as Foundation Professor of Physiology and Anatomy at the University of Sydney. At the time he was Assistant-Professor of Physiology in the University of Edinburgh. He initiated the building of the Sydney Medical School in Scottish Tudor Gothic style. He attracted notable figures to Sydney Medical School, such as Dr Robert Scot Skirving. The original medical school (now the Anderson Stuart Building) continues today as the pre-clinical medical school of the University of Sydney. Its stained glass windows and many busts of distinguished figures in the history of medicine are a constant reminder of the history of medicine. The building with its gothic architecture and echoes of northern Britain has given generations of Sydney medical students a powerful message, that they were part of an ancient and noble profession. The recruitment of Edinburgh academics to Sydney ended with Professor CG Lambie who retired in 1956. The 1950s were a watershed between the Edinburgh heritage and the Australian future.

BREVE VISIÓN DE LOS PRINCIPALES PROTAGONISTAS DE LA ESCUELA INGLESA DE ATENCIÓN PRIMARIA DURANTE EL ÚLTIMO MEDIO SIGLO

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Carlota Blanco Rubio

2016-01-01

Full Text Available El objetivo es hacer un breve repaso de las influencias, el núcleo duro de perfil clínico, la enfermería científica y el presente de la Escuela Inglesa de Atención Primaria durante los últimos sesenta años. Entendemos que la influencia intelectual más profunda fue la de Archie Cochrane. Entre los médicos de familia componentes del núcleo duro destacamos a John Fry (1922- 1994, David Metcalfe y Julian Tudor Hart. Por otra parte, Lisbeth Hockey (1918-2004, doctora en enfermería, fue la responsable durante varios años de la Unidad de Investigación en Cuidados de la Universidad de Edimburgo. Las fuentes de investigación historiográfica de la enfermería inglesa en internet son notables. Finalmente, Trisha Greenhalgh, médica de familia, investigadora y docente en la Universidad de Oxford, está lanzando a la vieja Escuela Inglesa de Atención Primaria hacia la mediana del siglo XXI.

Pretextos y contextos olvidados : arte, ciencia y técnica en algunos tratados contables de la Inglaterra Tudor

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Juan Lanero Fernández

2009-06-01

Full Text Available El presente estudio pretende ver, desde una perspectiva amplia, los dos tratados de James Peele (1553, 1569. The pathe waye (1569, en su conjunto, se aparta de forma considerable de los tratados publicados con anterioridad; y por lo que se refiere a su primer libro (1553, dispone de suficientes rasgos diferenciadores para que en el todo y en las partes sea una pieza bien distinta de tratados anteriores. Algunos detalles de The maner and fourme provienen directamente de Pacioli o a través del primer tratado inglés de partida doble, obra de Hugh Oldcastle (1543, o de Ympyn. La influencia de éste, por su tratado holandés/francés (1543 y la traducción inglesa (1547, se aprecia en The pathe waye en el cierre del Libro Mayor y en la forma que ambos tienen de tratar las operaciones de trueque. Es un hecho que Manzoni (1540 está presente en The moner and fourme en lo tocante a la numeración de las partidas en el Diario y a su referencia en el texto. Por lo que se refiere a la organización de los libros de cuentas, también existen resonancias de Weddington. La excelencia es la cualidad que destaca en los dos tratado, en particular en The pathe waye. Peele supo recoger en sus libros las tendencias más innovadoras de la partida doble provenientes de los autores que le precedieron. Está demostrado que Mellis manejó, aunque no siempre de forma apropiada, los tratados de Peele para su reedición de Oldcastle (1588. Y se puede decir lo mismo de John Carpenter (1632. Pele comprendió los verdaderos principios de la partida doble; pero sus excelentes tratados se quedaron injustamente olvidados en la penumbra de los pretextos, la competencia de los contextos y casi ningún paratexto que los valorara en su justiprecio que fue, y sigue siendo, elevado.The present paper tries to have a look, from a broad perspective, at James Peele's two treatises (1553, 1569. The pathe waye (1569, as a whole, drifts away considerably from the treatises previously published; and as far as the first book is concerned (1553, it has enough differentiating features to be, as a whole and in detall, a total different production from previous treatises too. Nevertheless, some details of The maner and fourme come either directly from Pacioli's or through the first English treatise by double entry, Hugh Oldcastle's (1543 or Ympyn's. The latter's influence through the Dutch/French version (1543 and its English translation (1547 can be seen either in the closure of the Ledger in The pathe waye or in the way both deal with barter transactions. It is a fact that Manzoni's (1540 is present in The maner and fourme as far as the numbering of the entries in the journal and its reference in the text is concerned. And the organization of the account books there are echoes from Weddington.Excellence is the outstanding quality in both treatises, particularly in The pathe waye. Peele was wise enough to gather in his books the innovating double-entry tendencies coming from the preceding authors. It is evident that Mellis handled, though not always in the appropriate way, Peele's treatises for his Oldcastle's reedition (1588; and the same thing applies to John Carpenter (1632. Peele properly understood the true principles informing the double entry system. Unfortunately, his two superb treatises were unfairly forgotten in the darkness of the pretexts, the competente of the contexts and barely any paratext assessing their value which was, and still is, high.

Infrared spectroscopy of the mineralogy of coprolites from Brean Down: evidence of past human activities and animal husbandry

Science.gov (United States)

Allen, Samantha D. M.; Almond, Matthew J.; Bell, Martin G.; Hollins, Peter; Marks, Sonja; Mortimore, Joanne L.

2002-03-01

The mineralogy of 11 concretions from the Bronze Age settlement horizons at Brean Down near Weston-super-Mare, Somerset, UK, has been examined by infrared spectroscopy. The concretions are found to contain calcite and apatite and, in some cases, quartz. Four further concretions from the later Iron Age Meare Village, soil samples from Brean Down and mineralised samples of known faecal origin from a cesspit within the Tudor Merchant's house in Tenby have been similarly examined. It is found that all samples contain calcite, but only the concretions and the Tenby cesspit samples contain apatite. None of the soil samples contain apatite, although these are relatively high in quartz. This suggests that the concretions are coprolites and that the apatite has a biological origin in small bone fragments. The infrared study is backed up by scanning electron microscopy which confirms the presence of phosphorus in the coprolite samples and shows a morphology suggestive of the presence of bone fragments; it is likely, therefore, that the coprolites result from a carnivore—most probably from dogs. The findings show the usefulness of infrared spectroscopy for the rapid identification of mineralised coprolitic material from archaeological sites.

Architecture, religion, and tuberculosis in Sainte-Agathe-des-Monts, Quebec.

Science.gov (United States)

Adams, Annmarie; Poutanen, Mary Anne

2009-01-01

This paper explores the architecture of the Mount Sinai Sanatorium in Sainte-Agathe-des-Monts (Qc) to disentangle the role of religion in the treatment of tuberculosis. In particular, we analyze the design of Mount Sinai, the jewel in the crown of Jewish philanthropy in Montreal, in relation to that of the nearby Laurentian Sanatorium. While Mount Sinai offered free treatment to the poor in a stunning, Art Deco building of 1930, the Protestant hospital had by then served paying patients for more than two decades in a purposefully home-like, Tudor-revival setting. Using architectural historian Bernard Herman's concept of embedded landscapes, we show how the two hospitals differed in terms of their relationship to site, access, and, most importantly, to city, knowledge, and community. Architects Scopes & Feustmann, who designed the Laurentian hospital, operated an office at Saranac Lake, New York, America's premier destination for consumptives. The qualifications of Mount Sinai architects Spence & Goodman, however, derived from their experience with Jewish institutions in Montreal. Following Herman's approach to architecture through movement and context, how did notions of medical therapy and Judaism intersect in the plans of Mount Sinai?

Chromatoid Body Protein TDRD6 Supports Long 3' UTR Triggered Nonsense Mediated mRNA Decay.

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Grigorios Fanourgakis

2016-05-01

Full Text Available Chromatoid bodies (CBs are spermiogenesis-specific organelles of largely unknown function. CBs harbor various RNA species, RNA-associated proteins and proteins of the tudor domain family like TDRD6, which is required for a proper CB architecture. Proteome analysis of purified CBs revealed components of the nonsense-mediated mRNA decay (NMD machinery including UPF1. TDRD6 is essential for UPF1 localization to CBs, for UPF1-UPF2 and UPF1-MVH interactions. Upon removal of TDRD6, the association of several mRNAs with UPF1 and UPF2 is disturbed, and the long 3' UTR-stimulated but not the downstream exon-exon junction triggered pathway of NMD is impaired. Reduced association of the long 3' UTR mRNAs with UPF1 and UPF2 correlates with increased stability and enhanced translational activity. Thus, we identified TDRD6 within CBs as required for mRNA degradation, specifically the extended 3' UTR-triggered NMD pathway, and provide evidence for the requirement of NMD in spermiogenesis. This function depends on TDRD6-promoted assembly of mRNA and decay enzymes in CBs.

Performance of a Hot-Dry Climate Whole-House Retrofit

Energy Technology Data Exchange (ETDEWEB)

Weitzel, E. [Alliance for Residential Building Innovation, Davis, CA (United States); German, A. [Alliance for Residential Building Innovation, Davis, CA (United States); Porse, E. [Alliance for Residential Building Innovation, Davis, CA (United States)

2014-06-01

The Stockton house retrofit is a two-story Tudor style single family deep retrofit in the hot-dry climate of Stockton, CA. The home is representative of a deep retrofit option of the scaled home energy upgrade packages offered to targeted neighborhoods under the pilot Large-Scale Retrofit Program (LSRP) administered by the Alliance for Residential Building Innovation (ARBI). Deep retrofit packages expand on the standard package by adding HVAC, water heater and window upgrades to the ducting, attic and floor insulation, domestic hot water insulation, envelope sealing, lighting and ventilation upgrades. Site energy savings with the deep retrofit were 23% compared to the pre-retrofit case, and 15% higher than the savings estimated for the standard retrofit package. Energy savings were largely a result of the water heater upgrade, and a combination of the envelope sealing, insulation and HVAC upgrade. The HVAC system was of higher efficiency than the building code standard. Overall the financed retrofit would have been more cost effective had a less expensive HVAC system been selected and barriers to wall insulation remedied. The homeowner experienced improved comfort throughout the monitored period and was satisfied with the resulting utility bill savings.

Performance of a Hot-Dry Climate Whole-House Retrofit

Energy Technology Data Exchange (ETDEWEB)

Weitzel, E.; German, A.; Porse, E.

2014-06-01

The Stockton house retrofit is a two-story tudor style single family deep retrofit in the hot-dry climate of Stockton, CA. The home is representative of a deep retrofit option of the scaled home energy upgrade packages offered to targeted neighborhoods under the pilot Large-Scale Retrofit Program (LSRP) administered by the Alliance for Residential Building Innovation (ARBI). Deep retrofit packages expand on the standard package by adding HVAC, water heater and window upgrades to the ducting, attic and floor insulation, domestic hot water insulation, envelope sealing, lighting and ventilation upgrades. Site energy savings with the deep retrofit were 23% compared to the pre-retrofit case, and 15% higher than the savings estimated for the standard retrofit package. Energy savings were largely a result of the water heater upgrade, and a combination of the envelope sealing, insulation and HVAC upgrade. The HVAC system was of higher efficiency than the building code standard. Overall the financed retrofit would have been more cost effective had a less expensive HVAC system been selected and barriers to wall insulation remedied. The homeowner experienced improved comfort throughout the monitored period and was satisfied with the resulting utility bill savings.

A CONCISE PRESENTATION OF THE FIRST LITERARY MAGANIZES PUBLISHED BY WOMEN WRITERS IN ROMANIA BETWEEN 1878 - 1947

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Carmen D. CARAIMAN

2014-05-01

Full Text Available In its early days Romanian feminine writing was influenced by the ideals of emancipation that animated the female population of our country. These ideals were shared and spread not only by feminist supporters, though this happened to a large extent, but also by outstanding men of letters (Eugen Lovinescu, Garabet-Ibraileanu and Tudor Vianu and others, who were receptive to the changing status of women in their contemporary society and encouraged it as such. The present paper focuses on what we are going to call “early feminine writing in Romania”, whereby we understand the early days of literature created by women in this country. Our goal is to identify the literary publications that gathered for the first time in Romania women writers who paid a particular interest in literature. Thus, our paper has a documentary value: it presents the main Romanian literary publications edited by the first women writers in our country, the aesthetic ideals promoted and shared by these magazines and the contribution brought by female authors to the wakening and shaping of the taste for writing and for literature amidst Romanian women in general.

Cooperative DNA and histone binding by Uhrf2 links the two major repressive epigenetic pathways

Science.gov (United States)

Pichler, Garwin; Wolf, Patricia; Schmidt, Christine S; Meilinger, Daniela; Schneider, Katrin; Frauer, Carina; Fellinger, Karin; Rottach, Andrea; Leonhardt, Heinrich

2011-01-01

Gene expression is regulated by DNA as well as histone modifications but the crosstalk and mechanistic link between these epigenetic signals are still poorly understood. Here we investigate the multi-domain protein Uhrf2 that is similar to Uhrf1, an essential cofactor of maintenance DNA methylation. Binding assays demonstrate a cooperative interplay of Uhrf2 domains that induces preference for hemimethylated DNA, the substrate of maintenance methylation, and enhances binding to H3K9me3 heterochromatin marks. FRAP analyses revealed that localization and binding dynamics of Uhrf2 in vivo require an intact tandem Tudor domain and depend on H3K9 trimethylation but not on DNA methylation. Besides the cooperative DNA and histone binding that is characteristic for Uhrf2, we also found an opposite expression pattern of uhrf1 and uhrf2 during differentiation. While uhrf1 is mainly expressed in pluripotent stem cells, uhrf2 is upregulated during differentiation and highly expressed in differentiated mouse tissues. Ectopic expression of Uhrf2 in uhrf1−/− embryonic stem cells did not restore DNA methylation at major satellites indicating functional differences. We propose that the cooperative interplay of Uhrf2 domains may contribute to a tighter epigenetic control of gene expression in differentiated cells. PMID:21598301

Control over the morphology and segregation of Zebrafish germ cell granules during embryonic development

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Nakkrasae La-Iad

2008-05-01

Full Text Available Abstract Background Zebrafish germ cells contain granular-like structures, organized around the cell nucleus. These structures share common features with polar granules in Drosophila, germinal granules in Xenopus and chromatoid bodies in mice germ cells, such as the localization of the zebrafish Vasa, Piwi and Nanos proteins, among others. Little is known about the structure of these granules as well as their segregation in mitosis during early germ-cell development. Results Using transgenic fish expressing a fluorescently labeled novel component of Zebrafish germ cell granules termed Granulito, we followed the morphology and distribution of the granules. We show that whereas these granules initially exhibit a wide size variation, by the end of the first day of development they become a homogeneous population of medium size granules. We investigated this resizing event and demonstrated the role of microtubules and the minus-end microtubule dependent motor protein Dynein in the process. Last, we show that the function of the germ cell granule resident protein the Tudor domain containing protein-7 (Tdrd7 is required for determination of granule morphology and number. Conclusion Our results suggest that Zebrafish germ cell granules undergo a transformation process, which involves germ cell specific proteins as well as the microtubular network.

Mapping Henry: Synchrotron-sourced X-ray fluorescence mapping and ultra-high-definition scanning of an early Tudor portrait of Henry VIII

Energy Technology Data Exchange (ETDEWEB)

Dredge, Paula; Ives, Simon [Art Gallery of New South Wales (AGNSW), Sydney, NSW (Australia); Howard, Daryl L.; Spiers, Kathryn M. [Australian Synchrotron, Clayton, VIC (Australia); Yip, Andrew [Art Gallery of New South Wales (AGNSW), Sydney, NSW (Australia); University of New South Wales, Laboratory for Innovation in Galleries, Libraries, Archives and Museums (iGLAM), National Institute for Experimental Arts, Sydney, NSW (Australia); Kenderdine, Sarah [University of New South Wales, Laboratory for Innovation in Galleries, Libraries, Archives and Museums (iGLAM), National Institute for Experimental Arts, Sydney, NSW (Australia)

2015-11-15

A portrait of Henry VIII on oak panel c. 1535 has recently undergone technical examination to inform questions regarding authorship and the painting's relationship to a group of similar works in the collections of the National Portrait Gallery, London, and the Society of Antiquaries. Due to previous conservation treatments of the painting, the conventional transmission X-radiograph image was difficult to interpret. As a result, the painting underwent high-definition X-ray fluorescence (XRF) elemental mapping on the X-ray fluorescence microscopy beamline of the Australian Synchrotron. Scans were conducted at 12.6 and 18.5 keV, below and above the lead (Pb) L edges, respectively. Typical scan parameters were 120 μm pixel size at 7 ms dwell time, with the largest scan covering an area 545 x 287 mm{sup 2} collected in 23 h (10.8 MP). XRF mapping of the panel has guided the conservation treatment of the painting and the revelation of previously obscured features. It has also provided insight into the process of making of the painting. The informative and detailed elemental maps, alongside ultra-high-definition scans of the painting undertaken before and after varnish and over-paint removal, have assisted in comparison of the finely painted details with the London paintings. The resolution offered by the combination of imaging techniques identifies pigment distribution at an extremely fine scale, enabling a new understanding of the artist's paint application. (orig.)

Mapping Henry: Synchrotron-sourced X-ray fluorescence mapping and ultra-high-definition scanning of an early Tudor portrait of Henry VIII

International Nuclear Information System (INIS)

Dredge, Paula; Ives, Simon; Howard, Daryl L.; Spiers, Kathryn M.; Yip, Andrew; Kenderdine, Sarah

2015-01-01

A portrait of Henry VIII on oak panel c. 1535 has recently undergone technical examination to inform questions regarding authorship and the painting's relationship to a group of similar works in the collections of the National Portrait Gallery, London, and the Society of Antiquaries. Due to previous conservation treatments of the painting, the conventional transmission X-radiograph image was difficult to interpret. As a result, the painting underwent high-definition X-ray fluorescence (XRF) elemental mapping on the X-ray fluorescence microscopy beamline of the Australian Synchrotron. Scans were conducted at 12.6 and 18.5 keV, below and above the lead (Pb) L edges, respectively. Typical scan parameters were 120 μm pixel size at 7 ms dwell time, with the largest scan covering an area 545 x 287 mm 2 collected in 23 h (10.8 MP). XRF mapping of the panel has guided the conservation treatment of the painting and the revelation of previously obscured features. It has also provided insight into the process of making of the painting. The informative and detailed elemental maps, alongside ultra-high-definition scans of the painting undertaken before and after varnish and over-paint removal, have assisted in comparison of the finely painted details with the London paintings. The resolution offered by the combination of imaging techniques identifies pigment distribution at an extremely fine scale, enabling a new understanding of the artist's paint application. (orig.)

Visualizadores de imagens médicas gratuitos: é possível trabalhar apenas com eles? Freeware medical image viewers: can we rely only on them?

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Filipe Ramos Barra

2010-10-01

Full Text Available OBJETIVO: Pesquisar visualizadores de imagens médicas gratuitos disponíveis na internet capazes de funcionar como cliente PACS (picture archiving and communication system e avaliar suas principais funções e a viabilidade do uso em computadores pessoais. MATERIAIS E MÉTODOS: Foi feita pesquisa, no Google e em sites especializados, por programas gratuitos disponíveis para o Windows. Foram encontrados cerca de 70, sendo 11 capazes de funcionar como cliente PACS, e selecionados seis destes para análise: ClearCanvas Workstation, KPACS, Onis, Synedra View Personal, Mito e Tudor DicomViewer. Com base nas necessidades dos autores, 16 funções foram avaliadas. RESULTADOS: Dos seis programas avaliados, dois possuem 10 das 16 funções avaliadas e um possui apenas duas. Três realizam MPR (reconstrução multiplanar, um realiza MIP (reconstrução por projeção de intensidade máxima, dois realizam VR (renderizações volumétricas, dois funcionam como servidor PACS, dois geram CDs, um realiza fusão de imagens, três permitem utilizar múltiplos monitores e apenas um não é compatível com Windows 7. CONCLUSÃO: Diversos programas gratuitos estão disponíveis e não existe nenhum completo. Cabe ao usuário analisar e selecionar o programa que melhor se enquadra nas suas necessidades, porém, os programas Onis, Synedra e ClearCanvas se destacam, cada um com suas peculiaridades. É totalmente viável o uso de programas gratuitos para o dia-a-dia do radiologista.OBJECTIVE: To search in the internet for freeware medical image viewers capable of running as a PACS (picture archiving and communication system client, and to evaluate their main functions as well as the feasibility of their use in personal computers. MATERIALS AND METHODS: The Google search engine and specialized sites were utilized in the search for freeware softwares for Windows. The authors have found about 70 and among them 11 were able to run as PACS clients. Six were selected for

Evidence against a germ plasm in the milkweed bug Oncopeltus fasciatus, a hemimetabolous insect

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Ben Ewen-Campen

2013-04-01

Primordial germ cell (PGC formation in holometabolous insects like Drosophila melanogaster relies on maternally synthesised germ cell determinants that are asymmetrically localised to the oocyte posterior cortex. Embryonic nuclei that inherit this “germ plasm” acquire PGC fate. In contrast, historical studies of basally branching insects (Hemimetabola suggest that a maternal requirement for germ line genes in PGC specification may be a derived character confined principally to Holometabola. However, there have been remarkably few investigations of germ line gene expression and function in hemimetabolous insects. Here we characterise PGC formation in the milkweed bug Oncopeltus fasciatus, a member of the sister group to Holometabola, thus providing an important evolutionary comparison to members of this clade. We examine the transcript distribution of orthologues of 19 Drosophila germ cell and/or germ plasm marker genes, and show that none of them localise asymmetrically within Oncopeltus oocytes or early embryos. Using multiple molecular and cytological criteria, we provide evidence that PGCs form after cellularisation at the site of gastrulation. Functional studies of vasa and tudor reveal that these genes are not required for germ cell formation, but that vasa is required in adult males for spermatogenesis. Taken together, our results provide evidence that Oncopeltus germ cells may form in the absence of germ plasm, consistent with the hypothesis that germ plasm is a derived strategy of germ cell specification in insects.

Evidence against a germ plasm in the milkweed bug Oncopeltus fasciatus, a hemimetabolous insect.

Science.gov (United States)

Ewen-Campen, Ben; Jones, Tamsin E M; Extavour, Cassandra G

2013-06-15

Primordial germ cell (PGC) formation in holometabolous insects like Drosophila melanogaster relies on maternally synthesised germ cell determinants that are asymmetrically localised to the oocyte posterior cortex. Embryonic nuclei that inherit this "germ plasm" acquire PGC fate. In contrast, historical studies of basally branching insects (Hemimetabola) suggest that a maternal requirement for germ line genes in PGC specification may be a derived character confined principally to Holometabola. However, there have been remarkably few investigations of germ line gene expression and function in hemimetabolous insects. Here we characterise PGC formation in the milkweed bug Oncopeltus fasciatus, a member of the sister group to Holometabola, thus providing an important evolutionary comparison to members of this clade. We examine the transcript distribution of orthologues of 19 Drosophila germ cell and/or germ plasm marker genes, and show that none of them localise asymmetrically within Oncopeltus oocytes or early embryos. Using multiple molecular and cytological criteria, we provide evidence that PGCs form after cellularisation at the site of gastrulation. Functional studies of vasa and tudor reveal that these genes are not required for germ cell formation, but that vasa is required in adult males for spermatogenesis. Taken together, our results provide evidence that Oncopeltus germ cells may form in the absence of germ plasm, consistent with the hypothesis that germ plasm is a derived strategy of germ cell specification in insects.

Gender-specific hierarchy in nuage localization of PIWI-interacting RNA factors in Drosophila

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Mikiko C Siomi

2011-08-01

Full Text Available PIWI-interacting RNAs (piRNAs are germline-specific small non-coding RNAs that form piRNA-induced silencing complexes (piRISCs by associating with PIWI proteins, a subclade of the Argonaute proteins predominantly expressed in the germline. piRISCs protect the integrity of the germline genome from invasive transposable DNA elements by silencing them. Multiple piRNA biogenesis factors have been identified in Drosophila. The majority of piRNA factors are localized in the nuage, electron-dense non-membranous cytoplasmic structures located in the perinuclear regions of germ cells. Thus, piRNA biogenesis is thought to occur in the nuage in germ cells. Immunofluorescence analyses of ovaries from piRNA factor mutants have revealed a localization hierarchy of piRNA factors in female nuage. However, whether this hierarchy is female-specific or can also be applied in male gonads remains undetermined. Here, we show by immunostaining of both ovaries and testes from piRNA factor mutants that the molecular hierarchy of piRNA factors shows gender-specificity, especially for Krimper (Krimp, a Tudor-domain containing protein of unknown function(s: Krimp is dispensable for PIWI protein Aubergine (Aub nuage localization in ovaries but Krimp and Aub require each other for their proper nuage localization in testes. This suggests that the functional requirement of Krimp in piRNA biogenesis may be different in male and female gonads.

Aggregation of SND1 in Stress Granules is Associated with the Microtubule Cytoskeleton During Heat Shock Stimulus.

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Shao, Jie; Gao, Fei; Zhang, Bingbing; Zhao, Meng; Zhou, Yunli; He, Jinyan; Ren, Li; Yao, Zhi; Yang, Jie; Su, Chao; Gao, Xingjie

2017-12-01

Stress granules (SGs) are dynamic dense structures in the cytoplasm that form in response to a variety of environmental stress stimuli. Staphylococcal nuclease and Tudor domain containing 1 (SND1) is a type of RNA-binding protein and has been identified as a transcriptional co-activator. Our previous studies have shown that SND1 is a component of the stress granule, which forms under stress conditions. Here, we observed that SND1 granules were often surrounded by ɑ-tubulin-microtubules in 45°C-treated HeLa cells at 15 min or colocalized with microtubules at 30 or 45 min. Furthermore, Nocodazole-mediated microtubule depolymerization could significantly affect the efficient recruitment of SND1 proteins to the SGs during heat shock stress. In addition, the 45°C heat shock mediated the enhancement of eIF2α phosphorylation, which was not affected by treatment with Nocodazole, an agent that disrupts the cytoskeleton. The intact microtubule cytoskeletal tracks are important for the efficient assembly of SND1 granules under heat shock stress and may facilitate SND1 shuttling between cytoplasmic RNA foci. Anat Rec, 300:2192-2199, 2017. © 2017 The Authors The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists. Copyright © 2017 The Authors The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

Ice in the Tropics: the Export of ‘Crystal Blocks of Yankee Coldness’ to India and Brazil

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Marc W. Herold

2011-11-01

Full Text Available The Boston natural ice trade thrived during 1830-70 based upon Frederic Tudor’s idea of combining two useless products – natural winter ice in New England ponds and sawdust from Maine’s lumber mills. Tudor ice was exported extensively to the tropics from the West Indies to Brazil and the East Indies as well as to southern ports of the United States. In tropical ice ports, imported natural ice was a luxury product, e.g., serving to chill claret wines (Calcutta, champagne (Havana and Manaus, and mint juleps (New Orleans and Savannah and used in luxury hotels or at banquets. In the temperate United States, natural ice was employed to preserve foods (cold storage and to cool water (Americans’ peculiar love of ice water. In both temperate and tropical regions natural ice found some use for medicinal purposes (to calm fevers. With the invention of a new technology to manufacture artificial ice as part of the Industrial Revolution, the natural ice export trade dwindled as import substituting industrialization proceeded in the tropics. By the turn of the twentieth century, ice factories had been established in half a dozen Brazilian port cities. All that remained of the once extensive global trade in natural ice was a sailing ship which docked in Rio Janeiro at Christmas time laden with ice and apples from New England.

Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage.

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Juliano, Celina E; Voronina, Ekaterina; Stack, Christie; Aldrich, Maryanna; Cameron, Andrew R; Wessel, Gary M

2006-12-01

Two distinct modes of germ line determination are used throughout the animal kingdom: conditional-an inductive mechanism, and autonomous-an inheritance of maternal factors in early development. This study identifies homologs of germ line determinants in the sea urchin Strongylocentrotus purpuratus to examine its mechanism of germ line determination. A list of conserved germ-line associated genes from diverse organisms was assembled to search the S. purpuratus genome for homologs, and the expression patterns of these genes were examined during embryogenesis by whole mount in situ RNA hybridization and QPCR. Of the 14 genes tested, all transcripts accumulate uniformly during oogenesis and Sp-pumilio, Sp-tudor, Sp-MSY, and Sp-CPEB1 transcripts are also uniformly distributed during embryonic development. Sp-nanos2, Sp-seawi, and Sp-ovo transcripts, however, are enriched in the vegetal plate of the mesenchyme blastula stage and Sp-vasa, Sp-nanos2, Sp-seawi, and Sp-SoxE transcripts are localized in small micromere descendents at the tip of the archenteron during gastrulation and are then enriched in the left coelomic pouch of larvae. The results of this screen suggest that sea urchins conditionally specify their germ line, and support the hypothesis that this mechanism is the basal mode of germ line determination amongst deuterostomes. Furthermore, accumulation of germ line determinants selectively in small micromere descendents supports the hypothesis that these cells contribute to the germ line.

Arthurian Toponymics: Folk Tradition or Antiquarian Invention? Review of the book: Lloyd, S. (2017. The Arthurian Place Names of Wales. Cardiff: University of Wales Press.

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Will Parker

2018-03-01

Full Text Available The article reviews Scott Lloyd’s survey of Arthurian place names in Wales, and the background to this material in the literature and scholarship of the modern and medieval periods. The reviewer presents an overview of Lloyd’s scope and methodology, situating it within the context of current trends in the wider field of Celtic studies. Lloyd’s survey shows that Arthurian toponymics is a modern as much as a medieval problem. The mutual influence between the map-makers on one hand, and the scholars and story-tellers on the other, is best regarded as a dynamic work-in-progress, rather than a passive snapshot of timeless folk tradition. Lloyd’s most significant discovery is the relative fluidity of Arthurian toponymics, with many of the place names in question first appearing on the cartographic or literary record no earlier than the 19th century. The case of the common Welsh place name Arthur’s Quoits or Coetan Arthur is considered, and Lloyd’s implication of a 17th century origin for this form is critically discussed. Attention is drawn to the alternating currents of scepticism and reconstructionism that have defined Arthurian scholarship and literature from the Tudor period onwards. The author then offers some concluding thoughts on Arthur’s “ontological ambiguity,” and the powerful stimulus this seems to have exerted on topographical and historiographical speculation, both modern and medieval.

Nuclease Tudor-SN Is Involved in Tick dsRNAMediated RNA Interference and Feeding but Not in Defense against Flaviviral or Anaplasma phagocytophilum Rickettsial Infection

Czech Academy of Sciences Publication Activity Database

Ayllón, N.; Naranjo, V.; Hajdušek, Ondřej; Villar, M.; Galindo, R. C.; Kocan, K.M.; Alberdi, P.; Šíma, Radek; Cabezas-Cruz, A.; Rückert, C.; Bell-Sakyi, L.; Kazimírová, M.; Havlíková, S.; Klempa, B.; Kopáček, Petr; de la Fuente, J.

2015-01-01

Roč. 10, č. 7 (2015), e0133038 E-ISSN 1932-6203 R&D Projects: GA MŠk(CZ) EE2.3.30.0032; GA ČR GP13-12816P EU Projects: European Commission(XE) 316304 Institutional support: RVO:60077344 Keywords : disease * proteins * cell lines Subject RIV: EC - Immunology Impact factor: 3.057, year: 2015

Altered ubiquitin causes perturbed calcium homeostasis, hyperactivation of calpain, dysregulated differentiation, and cataract.

Science.gov (United States)

Liu, Ke; Lyu, Lei; Chin, David; Gao, Junyuan; Sun, Xiurong; Shang, Fu; Caceres, Andrea; Chang, Min-Lee; Rowan, Sheldon; Peng, Junmin; Mathias, Richard; Kasahara, Hideko; Jiang, Shuhong; Taylor, Allen

2015-01-27

Although the ocular lens shares many features with other tissues, it is unique in that it retains its cells throughout life, making it ideal for studies of differentiation/development. Precipitation of proteins results in lens opacification, or cataract, the major blinding disease. Lysines on ubiquitin (Ub) determine fates of Ub-protein substrates. Information regarding ubiquitin proteasome systems (UPSs), specifically of K6 in ubiquitin, is undeveloped. We expressed in the lens a mutant Ub containing a K6W substitution (K6W-Ub). Protein profiles of lenses that express wild-type ubiquitin (WT-Ub) or K6W-Ub differ by only ∼2%. Despite these quantitatively minor differences, in K6W-Ub lenses and multiple model systems we observed a fourfold Ca(2+) elevation and hyperactivation of calpain in the core of the lens, as well as calpain-associated fragmentation of critical lens proteins including Filensin, Fodrin, Vimentin, β-Crystallin, Caprin family member 2, and tudor domain containing 7. Truncations can be cataractogenic. Additionally, we observed accumulation of gap junction Connexin43, and diminished Connexin46 levels in vivo and in vitro. These findings suggest that mutation of Ub K6 alters UPS function, perturbs gap junction function, resulting in Ca(2+) elevation, hyperactivation of calpain, and associated cleavage of substrates, culminating in developmental defects and a cataractous lens. The data show previously unidentified connections between UPS and calpain-based degradative systems and advance our understanding of roles for Ub K6 in eye development. They also inform about new approaches to delay cataract and other protein precipitation diseases.

A transcriptional coregulator, SPIN·DOC, attenuates the coactivator activity of Spindlin1.

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Bae, Narkhyun; Gao, Min; Li, Xu; Premkumar, Tolkappiyan; Sbardella, Gianluca; Chen, Junjie; Bedford, Mark T

2017-12-22

Spindlin1 (SPIN1) is a transcriptional coactivator with critical functions in embryonic development and emerging roles in cancer. SPIN1 harbors three Tudor domains, two of which engage the tail of histone H3 by reading the H3-Lys-4 trimethylation and H3-Arg-8 asymmetric dimethylation marks. To gain mechanistic insight into how SPIN1 functions as a transcriptional coactivator, here we purified its interacting proteins. We identified an uncharacterized protein (C11orf84), which we renamed SPIN1 docking protein (SPIN·DOC), that directly binds SPIN1 and strongly disrupts its histone methylation reading ability, causing it to disassociate from chromatin. The Spindlin family of coactivators has five related members (SPIN1, 2A, 2B, 3, and 4), and we found that all of them bind SPIN·DOC. It has been reported previously that SPIN1 regulates gene expression in the Wnt signaling pathway by directly interacting with transcription factor 4 (TCF4). We observed here that SPIN·DOC associates with TCF4 in a SPIN1-dependent manner and dampens SPIN1 coactivator activity in TOPflash reporter assays. Furthermore, knockdown and overexpression experiments indicated that SPIN·DOC represses the expression of a number of SPIN1-regulated genes, including those encoding ribosomal RNA and the cytokine IL1B. In conclusion, we have identified SPIN·DOC as a transcriptional repressor that binds SPIN1 and masks its ability to engage the H3-Lys-4 trimethylation activation mark. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Error review: Can this improve reporting performance?

International Nuclear Information System (INIS)

Tudor, Gareth R.; Finlay, David B.

2001-01-01

AIM: This study aimed to assess whether error review can improve radiologists' reporting performance. MATERIALS AND METHODS: Ten Consultant Radiologists reported 50 plain radiographs, in which the diagnoses were established. Eighteen of the radiographs were normal, 32 showed an abnormality. The radiologists were shown their errors and then re-reported the series of radiographs after an interval of 4-5 months. The accuracy of the reports to the established diagnoses was assessed. Chi-square test was used to calculate the difference between the viewings. RESULTS: On re-reporting the radiographs, seven radiologists improved their accuracy score, two had a lower score and one radiologist showed no score difference. Mean accuracy pre-education was 82.2%, (range 78-92%) and post-education was 88%, (range 76-96%). Individually, two of the radiologists showed a statistically significant improvement post-education (P < 0.01,P < 0.05). Assessing the group as a whole, there was a trend for improvement post-education but this did not reach statistical significance. Assessing only the radiographs where errors were made on the initial viewing, for the group as a whole there was a 63% improvement post-education. CONCLUSION: We suggest that radiologists benefit from error review, although there was not a statistically significant improvement for the series of radiographs in total. This is partly explained by the fact that some radiologists gave incorrect responses post-education that had initially been correct, thus masking the effect of the educational intervention. Tudor, G.R. and Finlay, D.B. (2001

«Ibernia fabulosa»: per una storia delle immagini dell'Irlanda in Italia

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Carlo Maria Pellizzi

2011-08-01

Full Text Available The essay traces the history of the relations between Italy and Ireland from the 7th century AD to the present. The Irish monks and pilgrims of the Dark Ages brought to the peninsula their hagiographic and legendary writings (such as the Purgatorium Sancti Patricii, which contributed – together with reminiscences from Classical antiquity – to an Italian vision of Ireland as a land of portents and wonders; while after the first Anglo-Norman conquest of the isle the propagandist writings of Gerald of Wales where for centuries the lens through which Ireland was seen in Italy. In the age of the Tudor conquest and Reformation the island slowly lost its fantastic connotations, becoming an asset in the Papal struggle against Anglican England. Later on, in the course of the Italian Risorgimento, the Italian leaders of the movement – such as Mazzini, Cavour, and Cattaneo - generally saw the nationalist movement in Ireland through English eyes, both because of their innate Anglophilia and for reasons of Realpolitik. Only in the 20th century, during WW1 and its aftermath, did Ireland and its national struggle receive in Italy less jaundiced and more sympathetic glances. In the late 1930s and in WW2 the Fascist regime tried to use Ireland as a lever against England; while in the 1970s and 1980s the reemergence of the Anglo-Irish conflict attracted again the interested attention of some sections of the Italian political scene, as did later the ‘peace process’. But through all this the Italian images of Ireland arising from present political realities kept being intertwined with the much older images of centuries gone by.  

Identification of hidden relationships from the coupling of hydrophobic cluster analysis and domain architecture information.

Science.gov (United States)

Faure, Guilhem; Callebaut, Isabelle

2013-07-15

Describing domain architecture is a critical step in the functional characterization of proteins. However, some orphan domains do not match any profile stored in dedicated domain databases and are thereby difficult to analyze. We present here an original novel approach, called TREMOLO-HCA, for the analysis of orphan domain sequences and inspired from our experience in the use of Hydrophobic Cluster Analysis (HCA). Hidden relationships between protein sequences can be more easily identified from the PSI-BLAST results, using information on domain architecture, HCA plots and the conservation degree of amino acids that may participate in the protein core. This can lead to reveal remote relationships with known families of domains, as illustrated here with the identification of a hidden Tudor tandem in the human BAHCC1 protein and a hidden ET domain in the Saccharomyces cerevisiae Taf14p and human AF9 proteins. The results obtained in such a way are consistent with those provided by HHPRED, based on pairwise comparisons of HHMs. Our approach can, however, be applied even in absence of domain profiles or known 3D structures for the identification of novel families of domains. It can also be used in a reverse way for refining domain profiles, by starting from known protein domain families and identifying highly divergent members, hitherto considered as orphan. We provide a possible integration of this approach in an open TREMOLO-HCA package, which is fully implemented in python v2.7 and is available on request. Instructions are available at http://www.impmc.upmc.fr/∼callebau/tremolohca.html. isabelle.callebaut@impmc.upmc.fr Supplementary Data are available at Bioinformatics online.

Shakespeare's history plays: Glorification or subversion of the dominant ideology?

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Kostić Milena M.

2014-01-01

Full Text Available Due to prioritizing Machiavellian power-craving aspirations over the inner voice of conscience, the majority of Shakespeare's rulers from the history plays suffers from the tragic 'dissociation of -sensibility' (Eliot 1921: 64, 66. Since the process of giving priority to public obligations instead to private inclinations is easily detected in the contemporary political scene, especially in the form of the imposed choice between legalism and morality, valuable ideas of the 20th century humanist critics (Kantorowicz, Rubenstein, Bauman, -Eliade, Hamvas are problematized in the paper. Their contemporary insights have been combined with the humanist philosophical and ethic tradition in the works of Ovid, Ficino, Mirandola, Bruno, More. The Renaissance spokesperson of this humanist tradition was Shakespeare; through the chronological survey of his history plays, the paper aims at revealing the author's criticism of the dominant Tudor ideology and neccessity for its subversion. Thus, the attitude presented by the representatives of the new historicist critical school, particularly Stephen Greenblatt, that the renaissance social institutions shaped, restricted and controlled an individual's conduct, whereby every attempt at subverting the dominant system was doomed to failure, is strongly criticized. The importance of 'the -historical sense' (Eliot 1963: 34, the concept relying on the permanent bond between the past and present, for the sake of creating a more humane future, has been recognized in Shakespeare's history plays, apart from the already mentioned 20th century humanist thinkers, also by the presentist critics at the beginning of the 21st -century, particularly Hughes Grady and Terence Hawkes (2007, who tirelessly claim, like Jan Kott before them (1990, that Shakespeare is our contemporary.

Education: 15. The Principles of Artistry in Musical Education

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Morari Marina

2018-03-01

Full Text Available The phenomenon is related to the unsafe world of feelings and brings together all meanings and cultural values. The performance of an artistic phenomenon is not possible without object-subjective relations, only in the frame of such kind of relations there can be established a cultural and spiritual communication, edification of the receptor. In the artistic phenomenon study, are known several approaches, which generate its understanding in several stages. The musical and artistic activity of the human being, as well as the product of this activity, can become determinant causes in education. The production of musical education depends on human skills and their degree of development. In all artistic activities - creation, interpretation, and reception - the "ordinary human being" valorises his own spiritual and artistic skills in order to contact directly with art. This article explores the conception of Tudor Vianu on the artistry, especially the analysis of the factors that determine the appearance of artistic phenomena and features of an artist The principles of artistry are deduced in education from the features of the artistic structure: the intuitiveness, the psychic depth of the empathies, the creative fantasy and the expressive power In the process of musical education, in pupil's personality that experience is formed that empathy, which cannot be acquired via contact with other phenomena / things. The understanding of the musical and artistic phenomena, in its totality, depends mostly on respecting a set of conditions. Applying the principles of artistry in musical education can ensure the joining of art with other human activities and the pre-eminence of art in everyone’s culture and life.

Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein.

Science.gov (United States)

Gray, Steven G; Iglesias, Antonio H; Lizcano, Fernando; Villanueva, Raul; Camelo, Sandra; Jingu, Hisaka; Teh, Bin T; Koibuchi, Noriyuki; Chin, William W; Kokkotou, Efi; Dangond, Fernando

2005-08-05

To effectively direct targeted repression, the class I histone deacetylases (HDACs) associate with many important regulatory proteins. In this paper we describe the molecular characterization of a member of the Jumonji domain 2 (JMJD2) family of proteins, and demonstrate its binding to both class I HDACs and the retinoblastoma protein (pRb). JMJD2 proteins are characterized by the presence of two leukemia-associated protein/plant homeodomain (LAP/PHD) zinc fingers, one JmjN, one JmjC (containing an internal retinoblastoma-binding protein 2 (RBBP2)-like sequence), and two Tudor domains. The first member of this group, JMJD2A, is widely expressed in human tissues and cell lines, and high endogenous expression of JMJD2A mRNA was found in several cell types, including human T-cell lymphotropic virus 1 (HTLV-1)-infected cell lines. JMJD2A and JMJD2B exhibit cell type-specific responses to the HDAC inhibitor trichostatin A. We show that the JMJD2A protein associates in vivo with pRb and class I HDACs, and mediates repression of E2F-regulated promoters. In HTLV-1 virus-infected cells, we find that JMJD2A binds to the viral Tax protein. Antibodies to JMJD2A recognize the native protein but also a half-sized protein fragment, the latter up-regulated in THP-1 cells during the G(2)/M phase of the cell cycle. The ability of JMJD2A to associate with pRb and HDACs and potentiate pRb-mediated repression of E2F-regulated promoters implies an important role for this protein in cell proliferation and oncogenesis.

Hearing and sports: a bidirectional interaction. [Audición y Control motor: Una relación recíproca].

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Johannes Vogel

2014-10-01

Full Text Available Introduction Motor control is crucially dependent on many sensory inputs that involve classically the proprioreceptors located in the tendons, joints and the muscle itself as well as inputs from the vestibular organ and eyes (Fitzpatrick and McCloskey, 1994. However, additional sensory input from the auditory system is often necessary to perform the sport-associated complex motor-tasks. This holds not only for team sports that requires continuous communication with the other players but also for others such as figure skating or gymnastics where the movements of the body need to be coordinated with music. In addition, hearing is also important for avoiding accidents e.g. during skiing to recognize other people on the same track. Conversely, specific sports wear used in these sports may negatively influence hearing as it was shown for ski helmets that reduce perception of safety-relevant frequencies (Ruedl, Kopp, Burtscher, Zorowka, Weichbold, Stephan, Koci and Seebacher, 2014; Tudor, Ruzic, Bencic, Sestan and Bonifacic, 2010. Moreover, the reaction time and force generated during voluntary contractions could be influenced by sound. For instance, runners closer to the starter's pistol at Olympic Games react sooner than runners farther away (Brown, Kenwell, Maraj and Collins, 2008. Finally, hearing could even influence the overall physical fitness as it might be reduced in deaf children (Hartman, Visscher and Houwen, 2007 although other studies could not confirm (Wierzbicka-Damska, Samolyk, Jethon, Wiercinska and Murawska-Cialowicz, 2005. In the elderly, sensory deficits such as poor vision and hearing may increase the risk of mobility decline (Viljanen, Kaprio, Pyykko, Sorri, Koskenvuo and Rantanen, 2009a; Viljanen, Kaprio, Pyykko, Sorri, Pajala, Kauppinen, Koskenvuo and Rantanen, 2009b.

The microRNA effector RNA-induced silencing complex in hidradenitis suppurativa: a significant dysregulation within active inflammatory lesions.

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Hessam, S; Sand, M; Skrygan, M; Bechara, Falk G

2017-09-01

Recently, we could show that the expression levels of the key regulators of the microRNA (miRNA) maturation and transport were dysregulated in inflamed hidradenitis suppurativa (HS) tissue (Heyam et al. in Wiley Interdiscip Rev RNA 6:271-289, 2015). The RNA-induced silencing complex (RISC) is the central element of the miRNA pathway and regulates miRNA formation and function. We investigated the expression of the RISC components, namely transactivation-responsive RNA-binding protein-1 (TRBP1), TRBP2, protein activator (PACT) of the interferon-induced protein kinase R, Argonaute RISC Catalytic Component-1 (AGO1) and Component-2 (AGO2), metadherin, and staphylococcal nuclease and Tudor domain-containing-1 (SND1) in inflamed HS tissue compared to healthy and psoriatic controls by real-time reverse transcription polymerase chain reaction. Expression levels of all investigated components were significantly lower in lesional HS skin (n = 18) compared to healthy controls (n = 10). TRBP1, PACT, AGO1, AGO2, and SND1 expression levels were significantly down-regulated in lesional HS skin compared to healthy-appearing perilesional skin (n = 7). TRBP2 and SND1 expression levels were significantly lower in healthy-appearing perilesional skin compared to healthy controls. In lesional HS skin, expression levels of PACT, AGO1, and AGO2 were significantly lower compared to psoriatic skin (n = 10). In summary, our data showed that all investigated components of RISC are dysregulated in the skin of HS patients, providing support for the hypothesis that miRNAs may have a pathological role in the inflammatory pathogenesis of HS.

The social and cultural background of initial forms of the English comedy and the development thereof before the age of Shakespeare

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Jovanović Slobodan D.

2011-01-01

of historical comedy would be that it is a play dealing with common or low people, and everyday events, which invariably ends happily, with laughter or forgiveness. It was important then to see what old pagan ritual practices, preserved among peasant folk and later adopted and modified by the Christian Church (Roman Catholic, of course, in these times, gradually developed even under the church roof, among the younger and less serious of church officials. Worth mentioning are later activities of wandering professional entertainers, and their getting some social status gradually. Due attention has been paid to the real beginning of the English drama, marked by the appearance of Miracle plays, short religious performances grouped in cycles, first performed by deacons or choir boys in church grounds, and later by members of guilds. Evolving in the late 15th and early 16th centuries then come Morality plays, Christian moral allegories in dramatic form, with the influence remaining strong in the 16th century, during the whole formative period of the English comedy. Renaissance then brought development of the so-called learned comedy under classical influence, which played a decisive role in the formation of English comedy. The age of Tudors and early Stuarts was an age of transition from feudal order to commercial capitalism. The absolute monarchy, established under the first Tudors, was a reflection and instrument of this transition, which had been a continuous process, begun as early as the 14th century. It means that the society of Shakespeare's times was a shifting, changing scene, in which the survivals of the medieval order of things existed side by side with new elements that were struggling into life. The comedy of Shakespeare's times, however, first took definite shape and artistic quality in the hands of the University Wits, a group of young authors with university education, of bourgeois origin, who wrote for popular as well as court theatres, and were in fact

Willingness to pay for highlands' agro-tourism recreational facility: A case of Boh Tea plantation, Cameron Highlands, Malaysia

International Nuclear Information System (INIS)

Herman M A Syamsul; Nur A'in C M; Ramachandran S; Ahmad S

2014-01-01

The increase in tourist demand for highland experience is inevitable. Cameron Highlands, established as a Tea Plantation Estate during the British Colonial era in 1929, has evolved into a major highland tourism destination providing a cool climatic experience coupled with scenic beauty in the midst of Tudor concept architecture which enhances the destinations historical value. Realising such tourism potential, the Boh Plantation management has provided a visitor centre as recreational facility for tourist utilisation. However, the absence in imposing an entrance fee has left a vacuum in determining the recreational economic value of this facility as the benefit of this agro-tourism product to tourists remains unknown. It would be important for the management to identify the benefit since the development and maintenance of the facility is costly. Hence, the purpose of this paper is to estimate the benefit of such establishment in highlands area by assessing visitor's Willingness to pay (WTP). The study examines, explores and debates the issues in a critical yet supportive environment especially highlands. The study obtained 179 usable questionnaires from visitors during weekends, weekdays and public holidays. The result showed that 59% of the visitors were willing to pay for the agro-tourism product. The WTP was estimated at RM 7.21 (€1.81). Three factors were found to be influencing WTP which were monthly income, years of education and perception on scenery. Although the study was conducted post development, the finding indicated the WTP for current management practise. Should the management change its style, it would also affect WTP and also the total economic value. Since WTP is established concept, the finding of the study reflects on the opportunities, barriers and challenges inherent in embracing post-disciplinary approaches to research and suggest ways to further enhance the approach

Willingness to pay for highlands' agro-tourism recreational facility: A case of Boh Tea plantation, Cameron Highlands, Malaysia

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A, Syamsul Herman M.; M, Nur A'in C.; S, Ahmad; S, Ramachandran

2014-03-01

The increase in tourist demand for highland experience is inevitable. Cameron Highlands, established as a Tea Plantation Estate during the British Colonial era in 1929, has evolved into a major highland tourism destination providing a cool climatic experience coupled with scenic beauty in the midst of Tudor concept architecture which enhances the destinations historical value. Realising such tourism potential, the Boh Plantation management has provided a visitor centre as recreational facility for tourist utilisation. However, the absence in imposing an entrance fee has left a vacuum in determining the recreational economic value of this facility as the benefit of this agro-tourism product to tourists remains unknown. It would be important for the management to identify the benefit since the development and maintenance of the facility is costly. Hence, the purpose of this paper is to estimate the benefit of such establishment in highlands area by assessing visitor's Willingness to pay (WTP). The study examines, explores and debates the issues in a critical yet supportive environment especially highlands. The study obtained 179 usable questionnaires from visitors during weekends, weekdays and public holidays. The result showed that 59% of the visitors were willing to pay for the agro-tourism product. The WTP was estimated at RM 7.21 (1.81). Three factors were found to be influencing WTP which were monthly income, years of education and perception on scenery. Although the study was conducted post development, the finding indicated the WTP for current management practise. Should the management change its style, it would also affect WTP and also the total economic value. Since WTP is established concept, the finding of the study reflects on the opportunities, barriers and challenges inherent in embracing post-disciplinary approaches to research and suggest ways to further enhance the approach.

Biochemical profiling of histone binding selectivity of the yeast bromodomain family.

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Qiang Zhang

2010-01-01

Full Text Available It has been shown that molecular interactions between site-specific chemical modifications such as acetylation and methylation on DNA-packing histones and conserved structural modules present in transcriptional proteins are closely associated with chromatin structural changes and gene activation. Unlike methyl-lysine that can interact with different protein modules including chromodomains, Tudor and MBT domains, as well as PHD fingers, acetyl-lysine (Kac is known thus far to be recognized only by bromodomains. While histone lysine acetylation plays a crucial role in regulation of chromatin-mediated gene transcription, a high degree of sequence variation of the acetyl-lysine binding site in the bromodomains has limited our understanding of histone binding selectivity of the bromodomain family. Here, we report a systematic family-wide analysis of 14 yeast bromodomains binding to 32 lysine-acetylated peptides derived from known major acetylation sites in four core histones that are conserved in eukaryotes.The histone binding selectivity of purified recombinant yeast bromodomains was assessed by using the native core histones in an overlay assay, as well as N-terminally biotinylated lysine-acetylated histone peptides spotted on streptavidin-coated nitrocellulose membrane in a dot blot assay. NMR binding analysis further validated the interactions between histones and selected bromodomain. Structural models of all yeast bromodomains were built using comparative modeling to provide insights into the molecular basis of their histone binding selectivity.Our study reveals that while not all members of the bromodomain family are privileged to interact with acetylated-lysine, identifiable sequence features from those that bind histone emerge. These include an asparagine residue at the C-terminus of the third helix in the 4-helix bundle, negatively charged residues around the ZA loop, and preponderance of aromatic amino acid residues in the binding pocket

Reduced ejection fraction heart failure – new data from multicenter studies and national registries regarding general and elderly populations: hopes and disappointments

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Crișan S

2018-04-01

Full Text Available Simina Crişan,1,2 Lucian Petrescu,1,2 Mihai Andrei Lazăr,1,2 Cristina Văcărescu,1,2 Alina-Ramona Nicola,1 Dragoş Cozma,1,2 Cristian Mornoş,1,2 Constantin Tudor Luca1,2 1Cardiology Department, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania; 2Institute of Cardiovascular Diseases, Timisoara, Romania Abstract: The evaluation of patients diagnosed with impaired systolic function heart failure represents a great challenge, in both the general and elderly population. We consider that elderly patients are the most severely affected by this disease that represents the final impact of cardiovascular disease continuum. Cardiovascular diseases are associated with serious morbidity and mortality, and considerable health care costs related to diagnosis and treatment. In this report we discuss some controversies regarding methods of heart failure evaluation as well as therapeutic steps and devices, including: reparatory therapeutic steps and initiation of therapy with loop diuretics, inconsistent dose titration for angiotensin-converting enzyme inhibitors/angiotensin receptor blocker and beta blockers, as well as novel therapies, such as angiotensin receptor neprilysin inhibitor and treatments that directly improve cardiomyocyte function. We conclude that, beyond technical progress, which is raising the cost of therapy for patients with heart failure, more careful monitoring of patient progress through clinical and paraclinical control visits, both at medical facilities and at home, would have greater impact and be more cost-effective. Physical therapy and promoting emotional and psychological wellbeing, to maintain a positive state of mind, contribute substantially to the quality of life and life expectancy, and are most important in elderly people who are most affected by dramatic reductions in wellbeing. Unfortunately, for many patients with severe impairment of left ventricular ejection fraction, these goals and therapeutic

[The Theory and Trend of Microhistory: History of Medicine].

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Sul, Heasim

2015-08-01

Microhistory, first developed in the 1970s, is the study of the past on a very small scale applying zoom-in methodology. Although microhistory had been introduced to Korea during the late 1990s, there still exists much of misunderstanding and confusions surrounding its nature. Microhistory is to be distinguished from the monographs which deal with petty subject, or from the history of everyday life, and from the case studies. In the field of the history of medicine, there are not many microhistories proper. Several works that claim to utilize microhistorical approach, could not be categorized as microhistory because they carry strong characteristics of macrohistory, specifically those of the disciplinary history or the case studies. The well known work of Harold J. Cook, Trials of an Ordinary Doctor, is not an exception. These studies fail to materialize the critical mind of microhistory that pursues to write a history from below and to restore the agency of obscure people. However, Guido Ruggiero's "The Strange Death of Margarita Marcellini," David Cressy's Travesties and Transgressions in Tudor and Stuart England, and Laurel Thatcher Ulrich's A Midwife's Tale clearly demonstrate the attributes, characteristics, and methodologies of microhistory. These studies well display the emphasis of microhistory, which reveal the complexity of early modern medicine, and the complicated function of individual relationships within each and every social setting. Recently, some scholars begin to suggest that the rigid definition of microhistory should be softened, arguing that there could be various types of microhistory. The history of medicine has many advantage of aptly applying many virtues of microhistory: the de-territoriality of diseases, the peculiar elements of the training and practice in hospitals and medical schools which call for anthropological survey, and the possibility of utilizing doctor's records that contain the confessions of the patients. Also, medical

Comparative heart failure profile over a 3-year period in a Romanian general hospital

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Pop D

2013-07-01

Full Text Available Dana Pop,1 Oana Maria Penciu,1 Adela Viviana Sitar-Taut,2 Dumitru Tudor Zdrenghea11Department of Cardiology, Clinical Rehabilitation Hospital, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania; 2"Babes-Bolyai" University, Cluj-Napoca, RomaniaBackground: Heart failure (HF has become an increasingly significant public health problem, associated with repeated hospitalizations, high costs, low quality of life, and decreased survival rate. The progress of the disease may be slowed if treatment is administered in accordance with current guidelines.Objectives: To compare the clinical profile of HF patients in a Romanian general hospital over a 3-year period.Methods and results: We studied two cohorts of patients admitted in the cardiology department of a rehabilitation hospital with a diagnosis of chronic HF New York Heart Association class II–IV. The first, in 2006, included 415 patients, 67.08 ± 10.59 years; the second, in 2009, included 500 patients, 67.31 ± 11.27 years. Considering all patients, the left ventricle ejection fraction (LVEF was not statistically different in the two cohorts. Compared to the 2006 cohort, the 2009 female cohort had higher LVEF (60.49% ± 13.41% vs 64.42% ± 13.79%, P < 0.05, while males over 65 years of age had lower LVEF (52.75% ± 15.02% vs 54.37% ± 15.23%, P = NS. For females, the probability of having LVEF ,45% was higher in 2006 (odds ratio = 1.573. HF with preserved LVEF was more common in females, both in 2006 (78.2% vs 54.2% and 2009 (87.2% vs 57.3%. In the 2009 cohort, LVEF was higher both in young patients (59.08% ± 14.22% vs 55.35% ± 14.92% and patients ≥ than 75 years of age (62.28% ± 13.81% vs 56.79% ± 14.81% compared to the 2006 cohort. Ischemic heart disease was the main underlying cause for HF in both cohorts.Conclusion: HF appeared to have the same clinical profile over a 3-year period. Females diagnosed with HF showed higher rates of preserved LVEF.Keywords: heart

"Advanced Technology (Quantum Mechanics Applied: The Science Behind Scriptural “Miracles” Excerpted from Scriptural and Secular Parallels"

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Eloise T. Choice

2017-12-01

Full Text Available Nanotechnology ( the mechanics of Quantum Theory is science, engineering, and technology conducted at the nanoscale, which is about 1 to 100 nanometers (1 billionth of a meter on the metric scale and involves imaging, measuring, modeling, and manipulating matter at this length scale (10-9. Therefore, nanotechnology, nanoscience and nanobiology involve the ability to see and to control individual atoms and molecules “the internal movement of matter with a certain external cause” (Tudor 227 at the nanoscale. The focus of this discussion is on the “miracles” described in the Judeo/ Christian Old Testament and the Christian New Testament. This paper postulates that God, Jesus, the heavenly angels, and their human emissaries (Moses, Aaron, Elijah, the disciples/apostles harnessed and applied similar scientific techniques (albeit far advanced beyond what mankind has done today to accomplish what are often referred to as “miracles” (secular “magic”. Therefore, what we in the religious communities have long referred to as “miracles” (as are recounted throughout Holy Scripture and non-canonical Scriptures were actually the result of advanced technologies applied. In the case of most of the Biblical “miracles,” the creation, restructuring and/or replication of physical matter were conducted at the atomic and molecular levels (10 -9. However, one in particular may have been conducted at the giga scale (10 9 when “… the sun stood still in the midst of heaven, and hasted not to go down about a whole day.” (Joshua 10:13. Nonetheless, this discussion proposes that these extraordinary events, manifestations of Divine intervention, were all accomplished in either of two ways: (1 A force of energy – referred to in Scripture as the Holy Spirit -- was tapped, harnessed, calibrated and channeled directly by way of Divine Word – the voices/ commands of God and Jesus -- to the intended target and (2 A force of energy – the Holy

Promoting physical activity for elders with compromised function: the Lifestyle Interventions and Independence for Elders (LIFE Study physical activity intervention

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Rejeski WJ

2013-09-01

Full Text Available W Jack Rejeski,1 Robert Axtell,2 Roger Fielding,3 Jeffrey Katula,1 Abby C King,4 Todd M Manini,5 Anthony P Marsh,1 Marco Pahor,5 Alvito Rego,6 Catrine Tudor-Locke,7 Mark Newman,8 Michael P Walkup,9 Michael E Miller9  On behalf of the LIFE Study Investigator Group 1Department of Health and Exercise Science, Wake Forest University, Winston-Salem, NC, 2Exercise Science Department, Southern Connecticut State University, New Haven, CT, 3Nutrtion, Exercise Physiology, and Sarcopenia Laboratory, Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA, 4Department of Health Research and Policy and Stanford Prevention Research Center, Department of Medicine, Stanford University School of Medicine, Palo Alto, CA, 5Department of Aging and Geriatric Research, College of Medicine, University of Florida, Gainesville, FL, 6Department of Internal Medicine, Northwestern School of Medicine, Chicago, IL, 7Pennington Biomedical Research Center, Baton Rouge, LA, 8Department of Epidemiology, University of Pittsburgh, Pittsburgh, PA, 9Department of Biostatistical Sciences, Division of Public Health Sciences, School of Medicine, Wake Forest University, Winston-Salem, NC, USA Abstract: The Lifestyle Interventions and Independence for Elders (LIFE Study is a Phase III randomized controlled clinical trial (Clinicaltrials.gov identifier: NCT01072500 that will provide definitive evidence regarding the effect of physical activity (PA on major mobility disability in older adults (70–89 years old who have compromised physical function. This paper describes the methods employed in the delivery of the LIFE Study PA intervention, providing insight into how we promoted adherence and monitored the fidelity of treatment. Data are presented on participants' motives and self-perceptions at the onset of the trial along with accelerometry data on patterns of PA during exercise training. Prior to the onset of training, 31.4% of

Into the Curriculum. "Brother, Can You Spare a Dime?" [and] Aboard the Titanic [and] Summarization [and] Public Speaking [and] Western Vocabulary [and] Acid Tests for the Library Media Center [and] Tasha Tudor, How Does Your Garden Grow? [and]"Baa-a-a-ck" to the Farm.

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School Library Media Activities Monthly, 1995

1995-01-01

Eight curriculum guides are provided for music and social studies; reading/language arts/social studies; reading/language arts; science; science/art; social studies. Objectives, resources, grade level, activity procedures, evaluation and follow-up suggestions are outlined for each activity. (JKP)

The 12th Edition of the Scientific Days of the National Institute for Infectious Diseases “Prof. Dr. Matei Bals” and the 12th National Infectious Diseases Conference

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Cristian-Mihail Niculae

2016-11-01

Tălăpan A9 Streptococcus pneumoniae meningitis presenting with normal CSF – case presentation Ramona Ștefania Popescu, Luminița Bradu, Dragoș Florea, Adrian Streinu-Cercel A10 Extrapulmonary manifestations of infection with Mycoplasma pneumoniae – study on 24 cases Daniela Anicuta Leca, Elena Bunea, Andra Teodor, Egidia Miftode A11 The molecular diagnosis of severe bacterial sepsis in pediatric population Mădălina Merișescu, Gheorghiță Jugulete, Adrian Streinu-Cercel, Dragoș Florea, Monica Luminos A12 Acute Staphylococcus aureus endocarditis with multiple septic complications in a patient with diabetes mellitus – case presentation Ramona Ștefania Popescu, Anamaria Dobrotă, Adina Ilie, Liliana Lucia Preoțescu A13 Is Streptococcus suis meningitis an under-diagnosed zoonosis? Adriana Hristea, Raluca Jipa, Nicoleta Irimescu, Irina Panait, Eliza Manea, Simona Merisor, Cristian Niculae, Daniela Tălăpan A14 Klebsiella pneumoniae isolated from blood. Antimicrobial resistance – past and present Liana Cătălina Gavriliu, Otilia Elisabeta Benea, Șerban Benea, Alexandru Rafila, Olga Dorobăț, Mona Popoiu A15 Antibiotics resistance in Staphylococcus aureus isolated from blood cultures Livia Dragonu, Augustin Cupşa, Iulian Diaconescu, Irina Niculescu, Lucian Giubelan, Florentina Dumitrescu, Andreea Cristina Stoian, Camelia Guţă, Simona Puiu A16 Predominance of CTX-M enzymes in extended-spectrum β-lactamase-producing Enterobacteriaceae in two hospitals of Quebec City Bunescu Irina, Marilyse Vallée, Ann Huletsky, Dominique K. Boudreau, Ève Bérubé, Richard Giroux, Jean Longtin, Yves Longtin, Michel G. Bergeron A17 Postoperative meningoencephalitis with Acinetobacter baumannii XDR – a therapeutic challenge - Case report Cleo Nicoleta Roșculeț, Dalila-Ana Toma, Catrinel Ciuca, Daniela Tălăpan, Cătălin Apostolescu, Andrei Rogoz, Andrei Stangaciu, Viorica Mitescu, Tudor Vladoiu, Doina Iovănescu A18 Septic arthritis with Burkholderia cepacia

Evaluation of Two Mobile Health Apps in the Context of Smoking Cessation: Qualitative Study of Cognitive Behavioral Therapy (CBT) Versus Non-CBT-Based Digital Solutions.

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Tudor-Sfetea, Carina; Rabee, Riham; Najim, Muhammad; Amin, Nima; Chadha, Mehak; Jain, Minal; Karia, Kishan; Kothari, Varun; Patel, Tejus; Suseeharan, Melanie; Ahmed, Maroof; Sherwani, Yusuf; Siddiqui, Sarim; Lin, Yuting; Eisingerich, Andreas B

2018-04-18

, these participants demonstrated preliminary changes in their smoking behavior, although this was in the context of our limited sample, not yet allowing for the finding to be generalizable. Our findings underscore the use of CBT in the context of mHealth apps as a feasible and potentially effective smoking cessation tool. mHealth apps must be well developed, preferably with an underlying behavioral change mechanism, to promote positive health behavior change. Digital CBT has the potential to become a powerful tool in overcoming current health care challenges. The present results should be replicated in a wider sample using the apps for a longer period so as to allow for generalizability. Further research is also needed to focus on the effect of greater personalization on behavioral change and on understanding the psychological barriers to the adoption of new mHealth solutions. ©Carina Tudor-Sfetea, Riham Rabee, Muhammad Najim, Nima Amin, Mehak Chadha, Minal Jain, Kishan Karia, Varun Kothari, Tejus Patel, Melanie Suseeharan, Maroof Ahmed, Yusuf Sherwani, Sarim Siddiqui, Yuting Lin, Andreas B Eisingerich. Originally published in JMIR Mhealth and Uhealth (http://mhealth.jmir.org), 18.04.2018.

Mazili and ruptaşi (and other social categories in the statistics of the 1817 census

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Valentin Tomuleţ

2017-12-01

Full Text Available At the time of annexation of Bessarabia to the Russian Empire, the Russian imperial institutions lacked concrete and reliable information on the number of people in the newly annexed province. The lack of statistical data for the entire province was also a problem for the regional administration, recently established in Chisinau, which for various reasons needed these data and constantly informed the imperial authorities about it. The population census became possible only in 1816-1817, when a number of concrete measures were taken in Bessarabia to establish an interim administration, to form of the first administrative and police institutions of power and some changes were made in the territorial administrative and customs and sanitary quarantine systems. In addition, the population had experienced the new regime of domination, the outflow of the population from the province to Moldova over the Prut was partially reduced, and the imperial administration was able to collect the first statistical data on the new annexed province. The results of the population census of 1817 were published in full in Russian, in 1907 by the historian I.N. Halippa, secretary of the Bessarabian Provincial Scientific Archival Committee. Subsequently, in 1920, the census was reproduced, this time in a more concise form, by Tudor Pamfile (only for the Hotin County, in 1929 – by professor of theology and history Constantin N. Tomescu, and in 1933 it was systematized by T. Porucic. The census forms contained the following components: 1. the name of districts and localities; 2. the economic situation of villages according to the category: a good state, b middle state (satisfactory, c insufficient state (unsatisfactory; 3. statistics of the clergy: priests and their widows, deacons and their widows, church clerks and their widows, psalm readers and their widows, and vergers and their widows; 4. mazili and their widows; 5. ruptaşi and their widows; 6. statistics of lower

PREFACE: EDS2010 Preface

Science.gov (United States)

Heggie, Malcolm I.

2011-03-01

the afternoon outing to Arundel Castle and dinner in the evening at Wiston House, a mansion of Tudor origin near Steyning, West Sussex. So a short audience-participation seminar was held in the conference room of the manor, covering the history of dislocations and the history of the conference series. We were also able to extend the appreciation of the life of Prof. Yuri Ossipyan (15 Feb 1931 - 10 Sep 2008) briefly given at EDS2008. EDS2010 continued the drive into graphene-based materials with a session devoted to them, and it gave immense pleasure to many of us who were his former students to dedicate a session to the work of Professor R Jones. We are grateful to his present and former co-workers who came and presented an impressive perspective on their work with him and a vision of a vigorous future for him in his retirement and for AIMPRO, the current Density Functional Theory code that derives from the one he established with his former student, Dr Patrick Briddon. For EDS2010 we made two minor modifications to the appearance of the conference: a central webpage www.eds-conferences.org, ably managed by our webmaster, Dr Gemma Haffenden, and a Facebook page, "EDS conference series", which Dr Amy Gandy runs enthusiastically. Amongst other things the conference photographs appear here. "I like this". In fact, currently 22 FB-ers "like this" and I am sure it will grow. Finally, it is a pleasure to acknowledge the significant contributions of Co-Chair, Prof. Marek Skowronski, Conference Manager, Dr Christopher Latham, and the editors of this volume, Drs Jon Goss and Chris Ewels, who in turn wish to thank Dr Alexis Vlandas for his help proof reading the articles. We all wish the best of luck to Prof. Philomela Khomninou and her team in the organisation of EDS2012.

New results concerning geophysical and geological-engineering data. Case study Telega, Romania

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Maftei, Raluca-Mihaela; Rusu, Emil; Cristea, Paul; Manj, Valeriu; Avram, Ovidiu; Tudor, Elena; Porumbescu, Constantina; Ciurean, Roxana

2010-05-01

New results concerning geophysical and geological-engineering data. Case study Telega, Romania R.Maftei, E.Rusu, P.Cristea, V.Manj, R.Ciurean, O.Avram, E.Tudor, C.Porumbescu Geological Institute of Romania, Geohazard, Bucharest, Romania (mafteir@yahoo.com) Geophysical tests The geoelectric investigation (October-November 2009) outlines horizontally the sliding area, and vertically the elements of the landslide surface - position, depth, shape, and the bedrock's relief. The quantitative interpretation of the resistivity geoelectrical vertical tests, and the correlation with the geological structure identified 3 sliding surfaces, from which only the upper one (2-6m depth) was known before the stability works. There were localized the rainfall waters circulation and accumulation zones, areas with high sliding risk. Same results were obtained in sliding zones, been localized the principal elements of the landslides, with practical implications in land instability and estimation of the evolution of the destructive phenomena mechanisms. With this study we try to quantify the complex relationship between the natural factors that generate the terrain instability phenomena and the intensity of the socio-economic effects, at a regional and local scale, by correlating the engineering geology information and geophysical data. Recent seismic research program (September 2009) conceived for "La Butoi" landslide, Telega locality, aims to a specific monitoring of the dynamic deformations, more active in the central part of the landslide, with reference to the shallow seismic refraction information obtained in the 2004 - 2005 period. The investigations were performed on a seismic lines network, and two seismic boundaries, in the shallow seismic section, were exhibited. As a result, one can observe the curvature tendency of the first arrivals sin-phase for the end-off shot devices, setting off the velocity increasing regime with depth; relative high variations and irregularities of