UNUSUAL BREEDING BY SEABIRDS AT MARION ISLAND ...

African Journals Online (AJOL)

In 1997/98, breeding at subantarctic Marion Island was exceptionally good for five species of seabirds capable of foraging over wide areas and for a tern. The number of king penguin Aptenodytes patagonicus chicks surviving to the start of spring in 1997 was considerably more than previously recorded. Greater numbers of ...

Measurement of cell proliferation in microculture using Hoechst 33342 for the rapid semiautomated microfluorimetric determination of chromatin DNA.

Science.gov (United States)

Richards, W L; Song, M K; Krutzsch, H; Evarts, R P; Marsden, E; Thorgeirsson, S S

1985-07-01

We report the development and characterization of a semiautomated method for measurement of cell proliferation in microculture using Hoechst 33342, a non-toxic specific vital stain for DNA. In this assay, fluorescence resulting from interaction of cell chromatin DNA with Hoechst 33342 dye was measured by an instrument that automatically reads the fluorescence of each well of a 96-well microtiter plate within 1 min. Each cell line examined was shown to require different Hoechst 33342 concentrations and time of incubation with the dye to attain optimum fluorescence in the assay. In all cell lines, cell chromatin-enhanced Hoechst 33342 fluorescence was shown to be a linear function of the number of cells or cell nuclei per well when optimum assay conditions were employed. Because of this linear relation, equivalent cell doubling times were calculated from growth curves based on changes in cell counts or changes in Hoechst/DNA fluorescence and the fluorimetric assay was shown to be useful for the direct assay of the influence of growth factors on cell proliferation. The fluorimetric assay also provided a means for normalizing the incorporation of tritiated thymidine ( [3H] TdR) into DNA; normalized values of DPM per fluorescence unit closely paralleled values of percent 3H-labelled nuclei when DNA synthesis was studied as a function of the concentration of rat serum in the medium. In summary, the chromatin-enhanced Hoechst 33342 fluorimetric assay provides a rapid, simple, and reproducible means for estimating cell proliferation by direct measurement of changes in cell fluorescence or by measurement of changes in the normalized incorporation of thymidine into DNA.

Comparison of UVA induced cytotoxicity by iodoHoechst isomers

International Nuclear Information System (INIS)

Karagiannis, T.C.; Lobachevsky, P.N.; Martin, R.F.

2003-01-01

Full text: Isomers of the DNA minor groove binding ligand, iodoHoechst, have been shown to sensitise DNA to cleavage by ultraviolet type A (UVA). The DNA damage has been attributed to formation of a carbon-centred radical upon UVA induced dehalogenation of the drugs. Comparison of the efficacy of the ligands in inducing DNA single strand breaks in plasmid DNA has indicated that the ortho isomer is more efficient than the para- and meta-isomers, mainly due to a greater cross-section for dehalogenation, and to some extent from increased efficiency of DNA damage per dehalogenation event. In the present study, the efficiency of dehalogenation and cytotoxicity of the three iodoHoechst isomers has been compared in human erythroleukemic, K562 cells. The uptake of the iodoHoechst compounds in K562 nuclei has been measured, and the photoefficiency of the cellular associated dehalogenation by UVA has been established for the three isomers. The results indicate that the sensitivity to UVA mediated dehalogenation is much higher for the ortho analogue compared to the para and meta-analogues. Values of the UVA D37 doses for the ortho, para and meta isomers are 49 ± 2, 327 ± 29 and 251 ± 32 J/m 2 , respectively. Clonogenic survival assays have been used to compare the efficiency of sensitisation of cells to UVA irradiation by the analogues. The ortho analogue exhibits higher efficiency compared to the meta and para analogues. The numbers of dehalogenation events required for cell kill have been calculated from the clonogenic survival at various levels of drug uptake, and the results for the ortho, para and meta isomers are 1.2x10 4 , 3.9x10 4 and 11.6x10 4 , respectively. These results indicate that the ortho analogue is the most efficient isomer in sensitising cell kill by UVA irradiation due to both the high quantum yield for dehalogenation and the higher cytotoxic efficiency of dehalogenation events

Zooplankton around Marion and Prince Edward Islands; 24 March 1976 to 25 November 1976 (NODC Accession 0000940)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Samples of zooplankton taken at 12 stations around Marion and Prince Edward Islands (47oS - 35oE) in March 1976 from the 'Marion-Dufresne' and at 2 stations in the...

[Optimization of labeling and localizing bacterial membrane and nucleus with FM4-64 and Hoechst dyes].

Science.gov (United States)

Wang, Jing; Han, Yanping; Yang, Ruifu; Zhao, Xingxu

2015-08-04

To observe cell membrane and nucleus in bacteria for subcellular localization. FM4-64 and Hoechst were dyed that can label cell membrane and nucleus, respectively. Both dyes were used to co-stain the membranes and nucleus of eight bacterial strains ( Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Yersinia pestis, Legionella pneumonia, Vibrio cholerae and Bacillus anthracis). E. coli was dyed with different dye concentrations and times and then observed by confocal fluorescence microscopic imaging. Fluorescence intensity of cell membrane and nucleus is affected by dye concentrations and times. The optimal conditions were determined as follows: staining cell membrane with 20 μg/mL FM4-64 for 1 min and cell nucleus with 20 μg/mL Hoechst for 20 min. Gram-negative bacteria were dyed better than gram-positive bacteria with FM4-64dye. FM4-64 and Hoechst can be used to stain membrane and nucleus in different types of bacteria. Co-staining bacterial membrane and nucleus provides the reference to observe cell structure in prokaryotes for studying subcellular localization.

Another Vision of Progressivism: Marion Richardson's Triumph and Tragedy.

Science.gov (United States)

Smith, Peter

1996-01-01

Profiles the career and contributions of English art teacher Marion Richardson (1892-1946). A dynamic and assertive woman, Richardson's ideas and practices changed British primary and secondary art teaching for many years. She often used "word pictures" (narrative descriptions of scenes or emotions) to inspire her students. (MJP)

Marion duPont Scott Equine Medical Center uses innovative lameness treatment

OpenAIRE

Lee, Kate

2009-01-01

Virginia Tech's Marion duPont Scott Equine Medical Center is now offering an equine lameness therapy that prevents further degeneration of the affected joint and offers a longer-lasting benefit than traditional steroid treatment.

Marion Richardson: "Art and the Child," a Forgotten Classic

Science.gov (United States)

Armstrong, Michael

2015-01-01

Marion Richardson was a revolutionary art teacher and schools inspector. First published in 1948, her book "Art and the Child" is one of the most remarkable educational documents of the period between the first and second world wars. This article reviews Richardson's philosophy and practice of art and suggests its continuing…

Aerial Photography and Imagery, Ortho-Corrected - 2008 Digital Orthophotos - Marion County

Data.gov (United States)

NSGIC Education | GIS Inventory — This data set is one component of a digital orthophoto coverage (DOI) over Marion County, FL. This dataset is comprised of 3-band 24-bit (red, green, and blue bands)...

Killer whales ( Orcinus orca ) at Marion Island, Southern Ocean ...

African Journals Online (AJOL)

Killer whales (Orcinus orca) were studied using data obtained on an opportunistic basis between 1973 and 1996 at Marion Island (46°54'S, 37°45'E) in the Southern Indian Ocean. A clear seasonal pattern of occurrence with the main peak between October and December was evident. Most killer whales were observed ...

Feeding ecology of three inshore fish species at Marion Island ...

African Journals Online (AJOL)

Three species of fish occur in the shallow inshore waters of Marion Island. Notothenia ... and then expressed as a percentage of the sum of all R.1. values for each ... also spends much of its time lying motionless on the bot- tom. Up to three H.

Synthesis of a Hoechst 32258 Analogue Amino Acid Building Block for Direct Incorporation of a Fluorescent High-Affinity DNA Binding Motif into Peptides

DEFF Research Database (Denmark)

Harrit, Niels; Behrens, Carsten; Nielsen, P. E.

2001-01-01

The synthesis of a new versatile "Hoechst 33258-like" Boc-protected amino acid building block for peptide synthesis is described. It is demonstrated that this new ligand is an effective mimic of Hoechst 33258 in terms of DNA affinity and sequence specificity. Furthermore, this minor groove binder...

Marion Industrial Substation project

International Nuclear Information System (INIS)

1992-04-01

Consumers Power, Inc. (CPI), proposes to construct a new distribution substation under the existing Bonneville Power Administration (BPA) Albay-Lebanon transmission line near the city of Albany in Linn County, Oregon. BPA is proposing to grant a new Point of Delivery to CPI at this substation. The purpose of the project is to serve the developing industrial needs in the Marion Industrial Park and on the 550 acres of nearby land within CPI's service area that are zoned for residential use. CPI prepared, and the Rural Electrification Administration (REA) has approved a Borrower's Environmental Report (BER) which addresses this action along with several other proposed projects in the CPI service area. Portions of this BER are summarized in this brief EA. BPA is preparing its own EA since Department of Energy National Environmental Policy Act (NEPA) guidelines require additional environmental concerns to be addressed than are required by REA's NEPA guidelines

Élites intellectuelles françaises, élites intellectuelles grecques et la « question de la langue » en Grèce dans les années 1920 et 1930 : le cas de Louis Roussel

OpenAIRE

Calvié, Julien

2016-01-01

Louis Roussel a tenu dans l’histoire du néo-hellénisme en France un rôle de pionnier. Son journal, Libre, qui parut régulièrement de décembre 1922 à juillet 1936, nous fournit une vision très riche et très vivante de la vie intellectuelle athénienne dans les années 1920 et 1930.Nous étudierons successivement la représentation des partisans de la langue savante et la représentation des démoticistes dans Libre. Nous conclurons en constatant que Louis Roussel a, sur « la question de la langue » ...

Summer diet of the Salvin's prion at sub-Antarctic Marion Island

African Journals Online (AJOL)

1988-02-14

Feb 14, 1988 ... Thirty-nine food samples were collected from Salvin's prions Pachyptila salvini at sub-Antarctic Marion Island,. Prince Edward Islands. ..... guide to foraging methods used by marine birds in. Antarctic and sub-Antarctic seas. BIOMASS Handbook. 24: 1-22. GRINDLEY, J.R. & LANE, S.B. 1979. Zooplankton.

Hoechst 33258 dye generates DNA-protein cross-links during ultraviolet light-induced photolysis of bromodeoxyuridine in replicated and repaired DNA

Energy Technology Data Exchange (ETDEWEB)

Guo Xicang; Morgan, W.F.; Cleaver, J.E.

1986-08-01

Substitution of bromodeoxyuridine for thymidine in the DNA of mammalian cells sensitizes them to a range of wavelengths of ultraviolet light. Cells are also sensitized to photochemical reactions involving dyes such as Hoechst 33258, which is used to produce differential staining of chromatids according to their bromodeoxyuridine content. Irradiation with 313 nm light of human and hamster cells containing bromodeoxyuridine in their DNA produced single-strand breaks but no DNA-protein cross-links. Irradiation with 360 nm light in the presence of Hoechst 33258 produced extensive DNA-protein cross-linkage as well as single-strand breaks. These cross-links were observed in DNA containing bromodeoxyuridine incorporated by either semiconservative or repair replication. When the protein was removed with proteinase K, bromodeoxyuridine in repair patches after irradiation by doses of ultraviolet (254 nm) light as low as 0.26 J/m/sup 2/ could readily be detected. Hoechst 33258-mediated photolysis, therefore, provides a sensitive new technique for measuring repair replication after ultraviolet light irradiation.

Flavour Profiling of 'Marion' and Thornless Blackberries by Instrumental and Sensory Analysis

Science.gov (United States)

The flavour of thornless blackberries grown in Pacific Northwest including 'Thornless Evergreen', 'Black Diamond', 'Black Pearl', 'Nightfall', ORUS 1843-3, 'Waldo', NZ 9351-4, and 'Chester Thornless' as well as 'Marion' was profiled by sensory evaluation and instrumental analysis. Sensory results sh...

Marion duPont Scott Equine Medical Center offers new treatment for lameness

OpenAIRE

Musick, Marjorie

2006-01-01

The Virginia-Maryland Regional College of Veterinary Medicine's Marion duPont Scott Equine Medical Center has begun offering a new therapy for treating lameness associated with osteoarthritis and cartilage damage in horses, a problem that affects all segments of the equine industry.

Effects on normal tissues during radiosensitization of Dalton's Lymphoma by the DNA ligand Hoechst 33342 in Balb/c mice

International Nuclear Information System (INIS)

Kalra, Namita; Sampath, Swapna; Adhikari, J.S.; Dwarakanath, B.S.

2014-01-01

Hoechst 33342 is a bisbenzimidazole derivative with AT specific minor groove DNA binding ability. Scavenging of free radicals and stabilization of macromolecular structure resulting in reduced induction of DNA damage contributes to radioprotection afforded by the ligand. Their ability to inhibit topoisomerases I and II, which play important roles in damage response pathways including DNA repair has been shown to sensitize tumor cells in vitro and in vivo. Due to its mutagenic and clastogenic potentials, damage to vital normal tissues are a matter of concern in deploying the ligand as adjuvant in radiotherapy. Therefore, we investigated the effects of the ligand in Dalton's Lymphoma (DL) bearing Balb/c mice by studying the local tumor control and animal survival, besides damage to normal tissues like bone marrow, kidney and testis. Hoechst 33342 (10 mg/kg b wt) was administered (i.v.) 1 h before focal irradiation (10 Gy) of the tumor (∼ 500 mm 3 ) grown on the hind leg of the mice. Partial response with a growth delay of 16 days (3 x initial volume) was seen following irradiation, while a complete response (cure; tumor-free survival) was observed in 88% mice following the combined treatment (Hoechst 33342+radiation); ligand alone had no significant effect. Although the ligand induced marginal degree of chromosomal aberrations in the bone marrow, it did not enhance aberrations induced by radiation further. In testes, the proportions of diploid, haploid and hypo-haploid cells as well as resting primary spermatocytes (RPS) were not significantly altered by either. In kidney, Hoechst 33342 alone or in combination with radiation did not cause significant damage to the proximal tubules and glomeruli. These observations suggest that radiosensitization of tumor by the DNA ligand Hoechst 33342 may not be associated with enhanced toxicity to bone marrow as well as proximal normal tissues. (author)

Volcanic ash deposition and long-term vegetation change on Subantarctic Marion Island

NARCIS (Netherlands)

Yeloff, D.; Mauquoy, D.S.; Barber, K.; Way, S.; van Geel, B.; Turney, C.S.M.

2007-01-01

A c. 5500 year record of peatland development and vegetation change was generated from a core recovered from an Agrostis magellanica peat bog on subantarctic Marion Island, using palynomorph, plant macrofossil, and tephra analyses. Two tephra horizons (both 17 cm thick) were identified and dated to

Heat Flow Data Cruise MD72 RV Marion Dufresne over the Mascarene Ridge

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Data were gathered by the R/V Marion Dufresne in May and June of 1992 over the Mascarene Ridge in the Indian Ocean on cruise MD72/MASCAFLUX. Heat flow measurements...

Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging.

Science.gov (United States)

Nakamura, Akinobu; Takigawa, Kazumasa; Kurishita, Yasutaka; Kuwata, Keiko; Ishida, Manabu; Shimoda, Yasushi; Hamachi, Itaru; Tsukiji, Shinya

2014-06-11

We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes.

Marion Milner, mysticism and psychoanalysis.

Science.gov (United States)

Sayers, Janet

2002-02-01

It is unusual to combine mysticism and psychoanalysis. Marion Milner, however, achieved precisely this. Through her self-analysis and analytic work with children and adults--and using as an illustration her own and others' imaginative ideas, paintings, doodles, drawings and pictures--she drew attention to the potential for health and creativity of undoing the obstacles to mystical experience of oneness with what is beyond or other than the self, which she sometimes called God, the unconscious or the id. This article seeks to explain and highlight this aspect of her contribution to, and continuing importance for, psychoanalytic theory and practice--particularly that associated with Winnicott--through detailing her early life and diary-keeping experiments, some of her psychoanalytic case histories during and after the Second World War, her work as an artist, ending with her travels and her involvement during the 1980s and 1990s with the Squiggle Foundation and British Association of Art Therapists.

The portrayal of J. Marion Sims' controversial surgical legacy.

Science.gov (United States)

Spettel, Sara; White, Mark Donald

2011-06-01

In the mid 1800s Dr. J. Marion Sims reported the successful repair of vesicovaginal fistulas with a technique he developed by performing multiple operations on female slaves. A venerated physician in his time, the legacy of Dr. Sims is controversial and represents a significant chapter in the mistreatment of African-Americans by the medical establishment. This review compares the modern debate surrounding his legacy with the presentation of his operation in widely consulted urological texts and journals. A literature review was performed of medical, sociological and periodical sources (1851 to the present) regarding J. Marion Sims and vesicovaginal fistula repair. During the last several decades, while the controversy around Dr. Sims' surgical development has produced a steady stream of articles in the historical and popular literature, relatively little mention is found in standard urology textbooks or journals. With increased public attention, some have debated the removal or modification of public tributes to Dr. Sims. This move has been countered by arguments against the validity of judging a 19th century physician by modern standards. While historians, ethicists and the popular press have debated Dr. Sims' legacy, medical sources have continued to portray him unquestionably as a great figure in medical history. This division keeps the medical profession uninformed and detached from the public debate on his legacy and, thus, the larger issues of ethical treatment of surgical patients. Copyright © 2011 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

Data report of the first cruise of the Marion Off-shore Ecological Study (MOES-1)

CSIR Research Space (South Africa)

Duncombe-Rae, CM

1989-01-01

Full Text Available The first cruise of the Marion Off-shore Ecological Study (MOES-I), during April and May 1987, was a multi-disciplinary effort aimed at gaining a further understanding of the relationships between productivity and the environment in the vicinity...

Leflunomide Aventis Pharma.

Science.gov (United States)

Kaplan, M J

2001-02-01

Hoechst Marion Roussel (HMR; now Aventis Pharma) launched leflunomide (HWA-486), an immunomodulator and a disease-modifying antirheumatic drug (DMARD), for the treatment of rheumatoid arthritis (RA) in the US in late 1998 [310118]. By August 2000, the compound had been launched extensively across all of Latin America and in all major European countries [380046]. The compound is also under preclinical investigationfor the prevention of transplant rejection [279727], [304402]. In 1998, HMR filed for approvalfor RA in Europe [279727]. In September 1998, the FDA approved leflunomide for the treatment of active RA in adults and it was launched shortly thereafter [298204], [299258], [310118]. In September 1999, the EU Commission accepted the view of the Committee for Proprietary Medicinal Products, published in May 1999 [326040], [337534], and gave approval for the use of leflunomide in RA in adults [339128]. Lehman Brothers has reported that EU launch was delayed by rare side effects including pancytopenia [354434]. In August 1998, the Arthritis Advisory Committee unanimously recommended that leflunomide be contraindicatedfor pregnancy, and that a pregnancy registry should be established to monitor possible teratogenic effects of the drug [296187]. Kyorin had a licence to develop leflunomide in Japan. Product approval was scheduled for 1998 [159079], but no development has been reported since 1994. Preclinical studies in an animal model of experimental allergic encephalomyelitis (EAE) have shown leflunomide to be a powerful immunosuppressant which may have potential in diseases such as multiple sclerosis [187881]. Leflunomide is rapidly processed in vivo to its active metabolite, A-771726 (RS-61980) [202941], [253615]. In 1996, leflunomide was designated as one of HMR's nine top-priority products, serving an unmet medical need and addressing a potential market in excess of US $500 million per year [221118].

Mythologizing Food: Marion Halligan’s non-fiction

Directory of Open Access Journals (Sweden)

Ulla Rahbek

2011-03-01

Full Text Available This paper discusses Marion Halligan’s non-fiction, particularly her writing on food: Those Women who go to Hotels, Eat my Words, Cockles of the Heart, Out of the Picture, and The Taste of Memory. The focus is on how Halligan deconstructs and reconstruct a mythology of food, in a Barthesian sense, revealing the contradictions at the heart of food mythology. The texts lay bare Halligan’s own personal and at times idiosyncratic mythology of food, where food is much more that just that. Venturing into areas of autobiography, memory, travel, place and gardens, this paper discusses how Halligan’s mythologizing of food doubles up, especially in her most recent food writing, as a rethinking and celebration of suburbia, which is figured as a site where nature and culture meet, and where paradise can be regained.

Hydrologic and water quality monitoring on Turkey Creek watershed, Francis Marion National Forest, SC

Science.gov (United States)

D.M. Amatya; T.J. Callahan; A. Radecki-Pawlik; P. Drewes; C. Trettin; W.F. Hansen

2008-01-01

The re-initiation of a 7,260 ha forested watershed study on Turkey Creek, a 3rd order stream, within the Francis Marion National forest in South Carolina, completes the development of a multi-scale hydrology and ecosystem monitoring framework in the Atlantic Coastal Plain. Hydrology and water quality monitoring began on the Santee Experimental...

Radiation sensitivity of tumour cells stained in vitro or in vivo with the bisbenzimide fluorochrome Hoechst 33342

Energy Technology Data Exchange (ETDEWEB)

Young, S D; Hill, R P [Ontario Cancer Inst., Toronto, ON (Canada)

1989-11-01

The DNA-binding bisbenzimide fluorochrome Hoechst 33342 is being used routinely in radio-biological studies to assess cell kinetic parameters and tumour blood flow. Results indicate that the amount of H33342 accumulated by cells may affect the radiation sensitivity of populations exposed to high concentrations of this fluorochrome, such as those required to achieve stoichiometric binding to DNA. (author).

78 FR 59913 - Revision of the Land Management Plan for the Francis Marion National Forest

Science.gov (United States)

2013-09-30

... telecommunication devices for the deaf (TDD) may call the Federal Information Relay Service (FIRS) at 1-800- 877... planning process can also be found on the Francis Marion National Forest Web site at www.fs.usda.gov/goto/scnfs/fmplan . SUPPLEMENTARY INFORMATION: Pursuant to the 2012 Forest Planning Rule (36 CFR Part 219...

Reading Chatelaine: Dr. Marion Hilliard and 1950s Women’s Health Advice

OpenAIRE

Mendes, Kaitlynn

2011-01-01

This article explores key themes and discourses surrounding women’s health advice written by Dr. Marion Hilliard between 1954 and 1957. Her works, published in eight Chatelaine articles and a 1957 book, are of particular significance because they demonstrate some of the many paradoxes Canadian women faced: women were told to stay at home and be housewives, but that they could have an identity of their own; that women too were sexual beings, but that their sexuality was confined within hete...

75 FR 39460 - Prevailing Rate Systems; Redefinition of the Chicago, IL; Fort Wayne-Marion, IN; Indianapolis, IN...

Science.gov (United States)

2010-07-09

...-AM21 Prevailing Rate Systems; Redefinition of the Chicago, IL; Fort Wayne-Marion, IN; Indianapolis, IN... wage area. These changes are based on recent consensus recommendations of the Federal Prevailing Rate... below. The Federal Prevailing Rate Advisory Committee (FPRAC), the national labor-management committee...

The biodiversity and species composition of the spider community of Marion Island, a recent survey (Arachnida: Araneae

Directory of Open Access Journals (Sweden)

T.T. Khoza

2005-12-01

Full Text Available Marion Island, the larger of the Prince Edward Islands, lies in the sub-Antarctic biogeographic region in the southern Indian Ocean. From previous surveys, four spider species are known from Marion. The last survey was undertaken in 1968. During this study a survey was undertaken over a period of four weeks on the island to determine the present spider diversity and to record information about the habitat preferences and general behaviour of the species present. Three collection methods (active search, Tullgren funnels and pitfall traps were used, and spiders were sampled from six habitat sites. A total of 430 spiders represented by four families were collected, Myro kerguelenesis crozetensis Enderlein, 1909 and M. paucispinosus Berland, 1947 (Desidae, Prinerigone vagans (Audouin, 1826 (Linyphiidae, Cheiracanthium furculatum Karsch, 1879 (Miturgidae and an immature Salticidae. The miturgid and salticid are first records. Neomaso antarticus (Hickman, 1939 (Linyphiidae was absent from samples, confirming that the species might have been an erroneous record.

The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica

CSIR Research Space (South Africa)

Sanyika, TW

2012-08-01

Full Text Available Marion Island is a Sub-Antarctic island made up of distinct ecological habitats based on soil physiochemical, plant cover and physical characteristics. The microbial diversity and ecological determinants in this harsh Sub-Antarctic environment...

A comparison of the effect of 5-bromodeoxyuridine substitution on 33258 Hoechst- and DAPI-fluorescence of isolated chromosomes by bivariate flow karyotyping

NARCIS (Netherlands)

Buys, C. H.; Mesa, J.; van der Veen, A. Y.; Aten, J. A.

1986-01-01

Application of the fluorescent DNA-intercalator propidium iodide for stabilization of the mitotic chromosome structure during isolation of chromosomes from V79 Chinese hamster cells and subsequent staining with the fluorochromes 33258 Hoechst or DAPI allowed bivariate flow karyotyping of isolated

Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage

Directory of Open Access Journals (Sweden)

P. Schendzielorz

2016-01-01

Full Text Available Adipose-derived stem cells (ASCs have been extensively studied in the field of stem cell research and possess numerous clinical applications. Cell labeling is an essential component of various experimental protocols and Hoechst 33342 (H33342 represents a cost-effective and easy methodology for live staining. The purpose of this study was to evaluate the labeling of rat ASCs with two different concentrations of H33342 (0.5 μg/mL and 5 μg/mL, with particular regard to usability, interference with cell properties, and potential DNA damage. Hoechst 33342 used at a low concentration of 0.5 μg/mL did not significantly affect cell proliferation, viability, or differentiation potential of the ASCs, nor did it cause any significant DNA damage as measured by the olive tail moment. High concentrations of 5 μg/mL H33342, however, impaired the proliferation and viability of the ASCs, and considerable DNA damage was observed. Undesirable colabeling of unlabeled cocultivated cells was seen in particular with higher concentrations of H33342, independent of varying washing procedures. Hence, H33342 labeling with lower concentrations represents a usable method, which does not affect the tested cell properties. However, the colabeling of adjacent cells is a drawback of the technique.

Gender and Hyper-Linear History in the Representation of the Female Australian Primary School Teacher in "Marion" (ABCTV, 1974)

Science.gov (United States)

May, Josephine

2018-01-01

Building on the author's previous work on Australian national cinema and schooling, this article explores the representation of the female primary school teacher in the television mini-series entitled "Marion" (Australian Broadcasting Commission, 1974). Using narrative analysis, it argues that this representation is disruptive of…

The eponymous Dr James Marion Sims MD, LLD (1813-1883).

Science.gov (United States)

West, M J; Irvine, L M

2015-02-01

Dr James Marion Sims was born in 1813 in Lancaster County, South Carolina. It was while pioneering numerous surgical procedures in Alabama that in 1849 he achieved the outstanding landmark in medical history of successfully, and consistently, repairing vesicovaginal fistulae. Sims soon developed a reputation as a fine surgeon, with new operations and techniques, using novel surgical instruments and his innovative approaches frequently published. Moving to New York City in 1853, he further established hospitals devoted entirely to women's health. Sims was controversial, with flamboyant descriptions of self-confident success, yet they were tempered with sober reflection of failure and loss. Today we remain with the Sims speculum and Sims position, eponymous tributes to his accomplishments as the 'Father of Gynaecology'. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Induction and detection of bystander effects after combined treatment of cells with 5-bromo-2 '-deoxyurine, Hoechst 33 258 and ultraviolet A light

NARCIS (Netherlands)

Xiao, Y.; de Feyter, E.; van Oven, C. H.; Stap, J.; Hoebe, R.; Havenith, S.; van Noorden, C. J. F.; Aten, J. A.

2004-01-01

Purpose: A combined treatment of cells with 5-bromo-2'-deoxyurine (BrdU), Hoechst 33 258 and ultraviolet A (UVA) light was used to introduce chromosomal aberrations in cells for the study of bystander effects in non-labelled cells. Materials and methods: Mixtures of BrdU-labelled and non-labelled

Report to the Minister of Environment Affairs on an environmental impact assessment of a proposed emergency landing facility on Marion Island - 1987

CSIR Research Space (South Africa)

Heymann, G

1987-01-01

Full Text Available of the Islands, with emphasis on the area of Marion Island which would be most seriously influenced, are described in some detail and mitigating measures discussed. The report concludes that the facility should not be constructed and so recommends...

Cloud amount/frequency, NITRATE and other data from MARION DUFRESNE in the NE Atlantic from 1987-01-14 to 1987-02-13 (NODC Accession 9000275)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Conductivity, Temperature and Depth (CTD) and other data were collected over one month period from NE Atlantic (limit-40 W) using Ship Marion Dufresne during...

Marion Milner e a clínica da criatividade: estilo, ética e técnica

OpenAIRE

Devito, Juliana

2015-01-01

Despertado pelo interesse de estudar o papel das artes na vida cotidiana e no espaço terapêutico e aprofundar o estudo sobre a criatividade nesses dois campos, o objetivo deste estudo é apresentar as ideias e os insights da psicanalista e artista inglesa Marion Milner acerca dos fenômenos psíquicos, do processo terapêutico e como eles ampliaram consideravelmente o âmbito da criatividade na Psicanálise. Veremos como sua obra dá ênfase ao papel da criatividade e da experiência estética no favor...

Annie Marion MacLean: “the mother of contemporary etnography” and pioneer in sociology distance learning

Directory of Open Access Journals (Sweden)

Silvia García Dauder

2008-05-01

Full Text Available The article introduces Anne Marion MacLean into the United States' scientific sociology at its very origins (1892, when the Department of Sociology of the Chicago University was created. It also puts MacLean in a network called "Women's School of Chicago", and the forgotten contributions of these social sciences' pioneers, submitted to the American Journal of Sociology, are analyzed. Two MacLean's key contributions are highlighted: the correspondence courses in teaching sociology and her research by means of participant observation in workplaces focusing on women's work.

Aerial gamma ray and magnetic survey: Marion quadrangle, Ohio. Final report

International Nuclear Information System (INIS)

1981-06-01

The Marion quadrangle covers a 7200 square mile area of central Ohio located within the Midwestern Physiographic Province. Up to 5000 feet of Paleozoic strata overlie the east dipping Precambrian basement. Flat lying Quaternary glacial sediments cover most of the surface within the quadrangle. A search of available literature revealed no known uranium deposits. Ninety-nine uranium anomalies were detected and are duscussed briefly. Radiometric data appear to reflect a preference for uranium occurrences in glacial moraine tills, and a minimum likelihood of occurrence in Paleozoic bedrock. Some of the largest anomalies appear to be culturally induced and no anomaly was considered to represent a significant amount of naturally occurring uranium. The magnetic data contrast somewhat with the existing structural interpretation of the area. The generally increasng magnetic gradient from west to east is interrupted by many features whose sources may be attributed to undefined lithologic and/or structural elements in the Precambrian basement

Fairy tales? Marion Jones, C.J. Hunter and the framing of doping in American newspapers

DEFF Research Database (Denmark)

Pfister, Gertrud Ursula; Gems, Gerald

2015-01-01

This article deals with the images, metaphors and narratives in the media coverage of doping in the United States. It presents a case study with a focus on Marion Jones, the most celebrated track athlete of the turn of the millennium, and her husband, C.J. Hunter, a shot put world champion...... under suspicion. At the same time, the 2000 Olympics can be considered a watershed in American anti-doping policy. The media portrayed Jones and Hunter as the Beauty and the Beast or Svengali and his victim, using a famous fairy tale and a well-known novel to capture attention, label the protagonists...

Flood-inundation maps for the Mississinewa River at Marion, Indiana, 2013

Science.gov (United States)

Coon, William F.

2014-01-01

Digital flood-inundation maps for a 9-mile (mi) reach of the Mississinewa River from 0.75 mi upstream from the Pennsylvania Street bridge in Marion, Indiana, to 0.2 mi downstream from State Route 15 were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Office of Community and Rural Affairs. The flood inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation, depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at the USGS streamgage on the Mississinewa River at Marion (station number 03326500). Near-real-time stages at this streamgage may be obtained on the Internet from the USGS National Water Information System at http://waterdata.usgs.gov/ or the National Weather Service (NWS) Advanced Hydrologic Prediction Service at http://water.weather.gov/ahps/, which also forecasts flood hydrographs at this site. Flood profiles were computed for the stream reach by means of a one-dimensional step-backwater model. The model was calibrated by using the current stage-discharge relation at the Mississinewa River streamgage, in combination with water-surface profiles from historic floods and from the current (2002) flood-insurance study for Grant County, Indiana. The hydraulic model was then used to compute seven water-surface profiles for flood stages at 1-fo (ft) intervals referenced to the streamgage datum and ranging from 10 ft, which is near bankfull, to 16 ft, which is between the water levels associated with the estimated 10- and 2-percent annual exceedance probability floods (floods with recurrence interval between 10 and 50 years) and equals the “major flood stage” as defined by the NWS. The simulated water-surface profiles were then combined with a Geographic Information System digital elevation model (derived from light detection and ranging (lidar) data having a 0.98 ft vertical accuracy and 4.9 ft

NMR characterization of the DNA binding properties of a novel Hoechst 33258 analogue peptide building block

DEFF Research Database (Denmark)

Bunkenborg, Jakob; Behrens, Carsten; Jacobsen, Jens Peter

2002-01-01

A novel aryl-bis-benzimidazole amino acid analogue of the DNA-binding compound Hoechst 33258 has recently been designed for incorporation in peptide combinatorial libraries by replacing the N-methylpiperazine group with a carboxyl group and the hydroxy group with an amino-methyl group. The DNA......-binding properties of the aryl-bis-benzimidazole monomer with the C-terminus derivatized with 3-(dimethylamino)-propylamine has been investigated in this paper by (1)H NMR studies of two different complexes with two different DNA sequences: A(5) d(5'-GCCA(5)CG-3'):d(5'-CGT(5)GGC-3') and A(3)T(3) d(5'-CGA(3)T(3)CG-3...... preference with the bis-benzimidazole moiety displaced toward the 3'-end from the center of the duplex. Two families of models of the complexes with A(5) and A(3)T(3) were derived with restrained molecular dynamics based on a large set of 70 and 61, respectively, intermolecular ligand NOEs. Both models give...

10Be application to soil development on Marion Island, southern Indian Ocean

International Nuclear Information System (INIS)

Haussmann, N.; Aldahan, A.; Boelhouwers, J.; Possnert, G.

2010-01-01

Marion Island, located in the southern Indian Ocean, constitutes the summit of an active shield volcano. It is a small terrestrial environment where glacially abraded bedrock became exposed c x 10 kyr ago. These conditions provide an interesting possibility for the assessment of 10 Be accumulation rates and their application to soil erosion studies on the island. 10 Be concentrations were measured in precipitation, soil profiles and an Azorella selago cushion plant. The data reveal a 10 Be precipitation flux several times higher than model prediction. Estimation of the 10 Be accumulation based on the soil inventory suggests a span between 2000 and 7000 yr. This time span is not in accordance with the accepted notion that the island was covered with ice about 10,000 yr ago and suggests either removal of 10 Be from the soil profile, an overestimated Holocene 10 Be-flux or a delayed soil development history. Our results provide new data on 10 Be concentrations from the sub-Antarctic islands and contribute towards enlarging the southern-hemisphere 10 Be database.

Did J. Marion Sims deliberately addict his first fistula patients to opium?

Science.gov (United States)

Wall, L Lewis

2007-07-01

American surgeon J. Marion Sims (1813-83) is regarded by many modern authors as a controversial figure because he carried out a series of experimental surgeries on enslaved African American women between 1846 and 1849 in an attempt to cure them of vesicovaginal fistulas, which they had all developed as a result of prolonged obstructed labor. He operated on one woman, Anarcha Westcott, thirty times before he successfully closed her fistula. Sims performed these fistula repair operations without benefit of anesthesia but gave these women substantial doses of opium afterwards. Several modern writers have alleged that Sims did this in order to addict them to the drug and thereby to enhance his control over them. This article examines the controversy surrounding Sims' use of postoperative opium in these enslaved surgical patients. The evidence suggests that although these women were probably tolerant to the doses of opium that he used, there is no evidence that he deliberately tried to addict them to this drug. Sims' use of postoperative opium appears to have been well supported by the therapeutic practices of his day, and the regimen that he used was enthusiastically supported by many contemporary surgeons.

County and Parish Boundaries, MFRDC has city limit boundaries for all municipalities in Dooly, Crisp, Macon, Taylor, Schley, Marion and Webster counties., Published in 2008, 1:63360 (1in=1mile) scale, Middle Flint Regional Development Commission.

Data.gov (United States)

NSGIC Regional | GIS Inventory — County and Parish Boundaries dataset current as of 2008. MFRDC has city limit boundaries for all municipalities in Dooly, Crisp, Macon, Taylor, Schley, Marion and...

The outlook of physician histories: J. Marion Sims and 'The Discovery of Anaesthesia'.

Science.gov (United States)

Rosenbloom, Julia M; Schonberger, Robert B

2015-12-01

The fact that doctors have a long tradition of writing medical history to interpret and direct their profession is well established. But readers (particularly modern physician readers) can also understand physician-authored histories as offering commentary and analysis of the world beyond medicine. In this essay, we offer a reading (perhaps a modern one) of J. Marion Sims's 1877 article, 'The Discovery of Anaesthesia' which exemplifies the stance of looking both inward and outward from the medical field. We begin by discussing Sims, including the complicated legacy he left as a physician. Next, we review late 19th-century history with a focus on Reconstruction. Finally, we show how the modern reader can use Sims's article both to trace the first use of ether and nitrous oxide for surgical anaesthesia and to provide a window into the 19th-century medical profession and the post-Civil War period. Through this study, we hope to show how to read both medicine and the world around it in physician histories. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

Address Points, MFRDC has address points for all municipalities in Dooly, Crisp, Macon, Taylor, Schley, Marion and Webster counties and for the counties as well., Published in 2008, 1:1200 (1in=100ft) scale, Middle Flint Regional Development Commission.

Data.gov (United States)

NSGIC Regional | GIS Inventory — Address Points dataset current as of 2008. MFRDC has address points for all municipalities in Dooly, Crisp, Macon, Taylor, Schley, Marion and Webster counties and...

A bulk tungsten tile for JET: Heat flux tests in the MARION facility on the power-handling performance and validation of the thermal model

International Nuclear Information System (INIS)

Mertens, Ph.; Altmann, H.; Chaumet, P.; Joffrin, E.; Knaup, M.; Matthews, G.F.; Neubauer, O.; Nicolai, D.; Riccardo, V.; Tanchuk, V.; Thompson, V.; Uhlemann, R.; Samm, U.

2011-01-01

In the frame of the ITER-like Wall (ILW) for the JET tokamak, a divertor row made of bulk tungsten material has been developed for the position where the outer strike point is located in most of the foreseen plasma configurations. In the absence of active cooling, this represents a formidable challenge when one considers the temperature reached by tungsten (T W,surf > 2000 deg. C) and the vertical gradient ∂T/∂z = 5 x 10 4 K/m. As the development is drawing to an end and most components are in production, actual 1:1 prototypes are exposed to an ion beam with a power density around 7 MW/m 2 on the plasma-facing surface. Advantage is taken of the flexibility of the MARION facility to bombard the tungsten stack under shallow angles of incidence (∼6 o ) with a powerful beam of ions and neutrals (>70 MW/m 2 on axis). The shallow angles are important, with respect to the toroidal wetted surface, for properly simulating the expected performance under actual tokamak conditions. The MARION tests have been used to validate for a few typical cases the thermal calculations that were steadily developed along with the tungsten tile and, at the same time, to gather information on the actual temperatures of individual components. The latter is an important factor to a finer estimation of the power handling capabilities.

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 2000-07-19 to 2000-08-16 (NODC Accession 0113576)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113576 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2000-07-19...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using Alkalinity titrator, CTD and other instruments from MARION DUFRESNE in the Indian Ocean from 2011-01-14 to 2011-02-20 (NODC Accession 0108369)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0108369 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2011-01-14 to...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from MARION DUFRESNE in the Indian Ocean from 2001-01-03 to 2001-01-26 (NODC Accession 0113577)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0113577 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2001-01-03...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 1998-08-18 to 1998-09-09 (NODC Accession 0113573)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113573 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1998-08-18...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 1998-12-05 to 1998-12-27 (NODC Accession 0113574)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113574 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1998-12-05...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using Alkalinity titrator, CTD and other instruments from MARION DUFRESNE in the Indian Ocean from 2009-12-19 to 2010-01-24 (NODC Accession 0109906)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0109906 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2009-12-19 to...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 2000-01-17 to 2000-01-27 (NODC Accession 0113575)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113575 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2000-01-17...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 1998-01-21 to 1998-02-19 (NODC Accession 0113570)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113570 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1998-01-21...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 2003-01-23 to 2003-02-17 (NODC Accession 0113571)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113571 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2003-01-23...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 2002-01-04 to 2002-02-01 (NODC Accession 0113578)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113578 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2002-01-04...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from MARION DUFRESNE in the Indian Ocean from 2005-01-11 to 2005-02-22 (NODC Accession 0108064)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0108064 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2005-01-11 to...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using Alkalinity titrator, CTD and other instruments from MARION DUFRESNE in the Indian Ocean from 2009-01-03 to 2009-02-12 (NODC Accession 0108065)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0108065 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2009-01-03 to...

Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from MARION DUFRESNE in the Indian Ocean and Savu Sea from 1992-02-17 to 1992-03-23 (NCEI Accession 0143946)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0143946 includes discrete sample and profile data collected from MARION DUFRESNE in the Indian Ocean and Savu Sea from 1992-02-17 to 1992-03-23. These...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 1993-01-23 to 1993-03-09 (NODC Accession 0115015)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115015 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1993-01-23 to 1993-03-09...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from MARION DUFRESNE in the Indian Ocean and Southern Oceans from 1996-02-20 to 1996-03-31 (NODC Accession 0115012)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0115012 includes discrete sample and profile data collected from MARION DUFRESNE in the Indian Ocean and Southern Oceans (> 60 degrees South) from...

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from MARION DUFRESNE in the Indian Ocean, Mozambique Channel and Southern Oceans from 2004-01-03 to 2004-02-09 (NODC Accession 0113572)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0113572 includes biological, chemical, discrete sample, optical, physical and profile data collected from MARION DUFRESNE in the Indian Ocean,...

The core competencies of James Marion Sims, MD.

Science.gov (United States)

Straughn, J Michael; Gandy, Roy E; Rodning, Charles B

2012-07-01

The concept of core competencies in graduate medical education was introduced by the Accreditation Council for Graduate Medical Education of the American Medical Association to semiquantitatively assess the professional performance of students, residents, practitioners, and faculty. Many aspects of the career of J. Marion Sims, MD, are exemplary of those core competencies: MEDICAL KNOWLEDGE: Author of the first American textbook related to gynecology. MEDICAL CARE: Innovator of the Sims' Vaginal Speculum, Sims' Position, Sims' Test, and vesico-/rectovaginal fistulorrhaphy; advocated abdominal exploration for penetrating wounds; performed the first cholecystostomy. PROFESSIONALISM: Served as President of the New York Academy of Medicine, the American Medical Association, and the American Gynecologic Society. INTERPERSONAL RELATIONSHIPS/COMMUNICATION: Cared for the indigent, hearthless, indentured, disenfranchised; served as consulting surgeon to the Empress Eugénie (France), the Duchess of Hamilton (Scotland), the Empress of Austria, and other royalty of the aristocratic Houses of Europe; accorded the National Order of the Legion of Honor. PRACTICE-BASED LEARNING: Introduction of silver wire sutures; adoption of the principles of asepsis/antisepsis; adoption of the principles of general anesthesia. SYSTEMS-BASED PRACTICE: Established the Woman's Hospital, New York City, New York, the predecessor of the Memorial Sloan-Kettering Center for the Treatment of Cancer and Allied Diseases; organized the Anglo-American Ambulance Corps under the patronage of Napoleon III. What led him to a life of clinical and humanitarian service? First, he was determined to succeed. His formal medical/surgical education was perhaps the best available to North Americans during that era. Second, he was courageous in experimentation and innovation, applying new developments in operative technique, asepsis/antisepsis, and general anesthesia. Third, his curiosity was not burdened by rigid

Dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean from 1999-01-04 to 1999-02-23 (NODC Accession 0113760)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113760 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1999-01-04 to 1999-02-23...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from MARION DUFRESNE in the Indian Ocean from 2008-01-05 to 2008-02-05 (NODC Accession 0081001)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0081001 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Indian Ocean from...

Dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using Alkalinity titrator, CTD and other instruments from the MARION DUFRESNE in the South Atlantic Ocean from 2008-02-07 to 2008-03-24 (NODC Accession 0112841)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112841 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the South Atlantic Ocean from 2008-02-07 to...

The medical ethics of Dr J Marion Sims: a fresh look at the historical record.

Science.gov (United States)

Wall, L L

2006-06-01

Vesicovaginal fistula was a catastrophic complication of childbirth among 19th century American women. The first consistently successful operation for this condition was developed by Dr J Marion Sims, an Alabama surgeon who carried out a series of experimental operations on black slave women between 1845 and 1849. Numerous modern authors have attacked Sims's medical ethics, arguing that he manipulated the institution of slavery to perform ethically unacceptable human experiments on powerless, unconsenting women. This article reviews these allegations using primary historical source material and concludes that the charges that have been made against Sims are largely without merit. Sims's modern critics have discounted the enormous suffering experienced by fistula victims, have ignored the controversies that surrounded the introduction of anaesthesia into surgical practice in the middle of the 19th century, and have consistently misrepresented the historical record in their attacks on Sims. Although enslaved African American women certainly represented a "vulnerable population" in the 19th century American South, the evidence suggests that Sims's original patients were willing participants in his surgical attempts to cure their affliction-a condition for which no other viable therapy existed at that time.

Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the MARION DUFRESNE in the Indian Ocean and Savu Sea from 1989-07-30 to 1989-09-09 (NODC Accession 0117679)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117679 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean and Savu Sea from 1989-07-30...

Monitoring Plasmodium falciparum growth and development by UV flow cytometry using an optimized Hoechst-thiazole orange staining strategy.

Science.gov (United States)

Grimberg, Brian T; Erickson, John J; Sramkoski, R Michael; Jacobberger, James W; Zimmerman, Peter A

2008-06-01

The complex life cycle of Plasmodium falciparum (Pf) makes it difficult to limit infections and reduce the risk of severe malaria. Improved understanding of Pf blood-stage growth and development would provide new opportunities to evaluate and interfere with successful completion of the parasite's life cycle. Cultured blood stage Pf was incubated with Hoechst 33342 (HO) and thiazole orange (TO) to stain DNA and total nucleic acids, respectively. Correlated HO and TO fluorescence emissions were then measured by flow cytometry. Complex bivariate data patterns were analyzed by manual cluster gating to quantify parasite life cycle stages. The permutations of viable staining with both reagents were tested for optimal detection of parasitized RBC (pRBC). Pf cultures were exposed to HO and TO simultaneously to achieve optimal staining of pRBC and consistent quantification of early and late stages of the replicative cycle (rings through schizonts). Staining of Pf nucleic acids allows for analysis of parasite development in the absence of fixatives, lysis, or radioactivity to enable examination of erythrocytes from parasite invasion through schizont rupture using sensitive and rapid assay procedures. Investigation of the mechanisms by which anti-malarial drugs and antibodies act against different Pf lifecycle stages will be aided by this cytometric strategy. (c) 2008 International Society for Advancement of Cytometry.

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Carbon dioxide (CO2) gas analyzer and other instruments from MARION DUFRESNE in the Arabian Sea, Bali Sea and others from 1991-01-05 to 1993-08-08 (NCEI Accession 0157100)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0157100 includes Surface underway, chemical, meteorological and physical data collected from MARION DUFRESNE in the Arabian Sea, Bali Sea, Gulf of...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from MARION DUFRESNE in the Indian Ocean and Southern Oceans from 1998-01-21 to 1998-12-28 (NODC Accession 0081003)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0081003 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Indian Ocean and Southern...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from MARION DUFRESNE in the Indian Ocean and Southern Oceans from 2000-01-15 to 2000-08-14 (NODC Accession 0081005)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0081005 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Indian Ocean and Southern...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from MARION DUFRESNE in the Arabian Sea, Gulf of Aden and others from 1999-10-18 to 1999-11-01 (NCEI Accession 0157283)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0157283 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Arabian Sea, Gulf of...

Efficacy of oxfendazole and fenbendazole against tortoise (Testudo hermanni) oxyurids.

Science.gov (United States)

Giannetto, S; Brianti, E; Poglayen, G; Sorgi, C; Capelli, G; Pennisi, M G; Coci, G

2007-04-01

Thirty-six tortoises (Testudo hermanni) with naturally acquired oxyurids infections were used to assess the anthelmintic efficacy of oxfendazole (Dolthene; Merial) and fenbendazole (Panacur; Hoechst Roussel Vet). Animals were randomly assigned to three groups (A, B, and C) based on sex and weight. Animals in group A (seven males and six females) were orally treated with oxfendazole at dose rate of 66 mg/kg, group B animals (nine males and eight females) were orally treated with fenbendazole at dose rate of 100 mg/kg, and group C animals (three males and three females) were not treated and served as controls. All animals were individually stabled in plexiglas boxes under controlled conditions of temperature, humidity, and light beginning 7 days pretreatment and continuing for the duration of the trial. Individual tortoises feces were examined daily by the McMaster technique and drugs efficacy was assessed by the fecal eggs count reduction (FECR) test. Both drugs showed 100% of FECR. However, oxfendazole reached this level 12 days after treatment, whereas 31 days after treatment were necessary to obtain the same stable result with fenbendazole. The two drugs were well tolerated by all the animals and no adverse reactions were observed after treatment.

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from MARION DUFRESNE in the Indian Ocean from 2013-02-10 to 2013-03-09 (NODC Accession 0116410)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0116410 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Indian Ocean from...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from MARION DUFRESNE in the Indian Ocean from 2016-01-08 to 2016-01-21 (NCEI Accession 0160553)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0160553 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Indian Ocean from...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from MARION DUFRESNE in the Indian Ocean from 2014-01-06 to 2014-02-19 (NCEI Accession 0157272)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0157272 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Indian Ocean from...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from MARION DUFRESNE in the Indian Ocean from 2015-01-07 to 2015-02-06 (NCEI Accession 0157289)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0157289 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Indian Ocean from...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from MARION DUFRESNE in the Indian Ocean from 2011-10-11 to 2011-11-21 (NODC Accession 0115604)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0115604 includes Surface underway, chemical, meteorological, optical and physical data collected from MARION DUFRESNE in the Indian Ocean from...

Thank you

CERN Multimedia

We have been deeply touched by the expressions of sympathy and affection from all the friends and colleagues of Frédéric Roussel at CERN. As it is impossible to answer each one, Baptiste, Bénédicte, Guylaine and I would like to express our gratitude to everyone who sent their condolences. Sylvie Roussel

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the MARION DUFRESNE in the Indian Ocean from 2009-01-04 to 2009-02-09 (NODC Accession 0108227)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108227 includes biological, chemical, meteorological, physical and underway - surface data collected from MARION DUFRESNE in the Indian Ocean from...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the MARION DUFRESNE in the Indian Ocean from 2011-01-15 to 2011-02-18 (NODC Accession 0114448)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0114448 includes biological, chemical, meteorological, physical and underway - surface data collected from MARION DUFRESNE in the Indian Ocean from...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the MARION DUFRESNE in the Indian Ocean from 2012-01-25 to 2012-03-07 (NODC Accession 0116411)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116411 includes biological, chemical, meteorological, physical and underway - surface data collected from MARION DUFRESNE in the Indian Ocean from...

Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Thin film type equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the MARION DUFRESNE in the Indian Ocean from 2009-12-24 to 2010-01-22 (NODC Accession 0108228)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108228 includes biological, chemical, meteorological, physical and underway - surface data collected from MARION DUFRESNE in the Indian Ocean from...

Water-resources programs and hydrologic-information needs, Marion County, Indiana, 1987

Science.gov (United States)

Duwelius, R.F.

1990-01-01

Water resources are abundant in Marion County, Indiana, and have been developed for public and industrial supply, energy generation, irrigation, and recreation. The largest water withdrawals are from surface water, and the two largest water uses are public supply and cooling water for electrical-generating plants. Water-resources programs in the county are carried out by Federal, State and local agencies to address issues of surface and groundwater availability and quality. The programs of each agency are related to the functions and goals of the agency. Although each agency has specific information needs to fulfill its functions, sometimes these needs overlap, and there are times when the same hydrologic information benefits all. Overlapping information needs and activities create opportunities for interagency coordination and cooperation. Such cooperation could lead to a savings of dollars spent on water-resources programs and could assure an improved understanding of the water resources of the county. Representatives from four agencies-- the Indiana Department of Environmental Management, the Indiana Department of Natural Resources, the Indianapolis Department of Public Works, and the U.S. Geological Survey--met four times in 1987 to describe their own water-resources programs, to identify hydrologic-information needs, and to contact other agencies with related programs. This report presents the interagency findings and is intended to further communication among water resource agencies by identifying current programs and common needs for hydrologic information. Hydrologic information needs identified by the agency representatives include more precise methods for determining the volume of water withdrawals and for determining the volume of industrial and municipal discharges to surface water. Maps of flood-prone areas need to be updated as more of the county is developed. Improved aquifer maps of the inter-till aquifers are needed, and additional observation

J. Marion Sims and the Vesicovaginal Fistula: Historical Understanding, Medical Ethics, and Modern Political Sensibilities.

Science.gov (United States)

Wall, L Lewis

To review the historical background surrounding the early work of Dr. J. Marion Sims, who developed the first consistently successful surgical technique for the repair of obstetric vesicovaginal fistulas by operating on a group of young, enslaved, African American women who had this condition between 1846 and 1849. Review of primary source documents on Sims and his operations, early 19th century clinical literature on the treatment of vesicovaginal fistula, the introduction of ether and chloroform anesthesia into surgical practice, and the literature on the early 19th century medical ethics pertaining to surgical innovation. The goals are to understand Sims's operations within the clinical context of the 1840s and to avoid the problems of "presentism," in which beliefs, attitudes, and practices of the 21st century are anachronistically projected backward into the early 19th century. The object is to judge Sims within the context of his time, not to hold him accountable to standards of practice which were not developed until a century after his death. A narrative of what Sims did is presented within the context of the therapeutic options available to those with fistula in the early 19th century. Review of the available material demonstrates that Sims' first fistula operations were legal, that they were carried out with express therapeutic intent for the purpose of repairing these women's injuries, that they conformed to the ethical requirements of his time, and that they were performed with the patients' knowledge, cooperation, assent, and assistance.

Aspm, a Key Element in Medulloblastoma Pathogenesis and a Novel Target for Treatment

Science.gov (United States)

2013-10-01

pathogenesis ( Wechsler -Reya and Scott, 1999; Kenney and Rowitch, 2000; Zurawel et al., 2000; Ellison et al., 2011; Hatten and Roussel, 2011; Roussel...Laboratories, Bar Harbor, ME, USA) to generate Aspm floxed (Aspmf/+) mice. Math-1 cre mice were generously shared by David Rowitch, MD, PhD, UCSF and...Robert Wechsler -Reya, PhD, 10 Sanford-Burnham Medical Research Institute, La Jolla, CA and have been previously described (Matei et al., 2005

Exubera. Inhale therapeutic systems.

Science.gov (United States)

Bindra, Sanjit; Cefalu, William T

2002-05-01

Inhale, in colaboration with Pfizer and Aventis Pharma (formerly Hoechst Marion Roussel; HMR), is developing an insulin formulation utilizing its pulmonary delivery technology for macromolecules for the potential treatment of type I and II diabetes. By July 2001, the phase III program had been completed and the companies had begun to assemble data for MAA and NDA filings; however, it was already clear at this time that additional data might be required for filing. By December 2001, it had been decided that the NDA should include an increased level of controlled, long-term pulmonary safety data in diabetic patients and a major study was planned to be completed in 2002, with the NDA filed thereafter (during 2002). US-05997848 was issued to Inhale Therapeutic Systems in December 1999, and corresponds to WO-09524183, filed in February 1995. Equivalent applications have appeared to date in Australia, Brazil, Canada, China, Czech Republic, Europe, Finland, Hungary, Japan, Norway, New Zealand, Poland and South Africa. This family of applications is specific to pulmonary delivery of insulin. In February 1999, Lehman Brothers gave this inhaled insulin a 60% probability of reaching market, with a possible launch date of 2001. The analysts estimated peak sales at $3 billion in 2011. In May 2000, Aventis predicted that estimated peak sales would be in excess of $1 billion. In February 2000, Merrill Lynch expected product launch in 2002 and predicted that it would be a multibillion-dollar product. Analysts Merril Lynch predicted, in September and November 2000, that the product would be launched by 2002, with sales in that year of e75 million, rising to euro 500 million in 2004. In April 2001, Merrill Lynch predicted that filing for this drug would occur in 2001. Following the report of the potential delay in regulatory filing, issued in July 2001, Deutsche Banc Alex Brown predicted a filing would take place in the fourth quarter of 2002 and launch would take place in the first

Dame Bug and her students: The science and environmental teaching of Edith Marion Patch

Science.gov (United States)

Bird, Mary Dickinson

What can we learn about science teaching from a dead entomologist? And what will this learning mean in the context of our own teaching, half a century after her death? This project is an exploration of the educational ideas and practices of Edith Marion Patch (1876-1954), a pioneering entomologist, educator, and environmentalist whose research and teaching shaped science learning among farmers, children, parents, teachers, and fellow scientists from the start of her career in 1903 until her retirement from professional endeavors in 1945. Patch was one of America's first university-trained female entomologists, the first woman president of the Entomological Society of America and one of the most respected scientists of her time. She was a beloved, bestselling author of more than one hundred natural history stories and books for young learners and dozens of nature and gardening articles for adults. She was also one of the earliest environmentalists to sound an alarm about the hazards of chemical pesticides, delivering her first address on the topic in 1906, one year before Rachel Carson was born. Because of the respect she earned in every milieu in which she worked, Edith Patch was uniquely positioned to influence a wide range of learners, from fellow scientists to home gardeners, to little children. Despite her accomplishments and her renown during her lifetime, Edith Patch is little remembered today. Yet her educational work may prove to be of particular relevance for our time, in which scientific literacy and environmental stewardship take on increasing urgency. Indeed, in the current climate of educational reform, which in some aspects reproduces the inquiry-based, experiential approach advocated by Patch and other education reformers a century ago, a close examination of Patch's teaching may prove to be of particular value. This study therefore seeks to fill the gap in our pedagogical memory regarding this eminent woman, considering the copious evidence of ways

Renovación de pasturas degradadas de kikuyo Pennisetum clandestinum, Hoechst, con labranza mínima en una región alto andina de Colombia I. Productividad forrajera.

Directory of Open Access Journals (Sweden)

García Indira Isis

2004-12-01

Full Text Available Se utilizaron tres métodos de renovación de pasturas degradadas: (Kikuyo sin labranza mínima, KSLM; Kikuyo con labranza mínima, KLM, y kikuyo con labranza mínima más la aplicación de fertilización química y siembra de tréboles, KLMFS en período lluvioso y seco; se incluyó como testigo (T el manejo tradicional de la pastura en la finca. La disponibilidad de forraje verde seco fue mayor en KLM y KLMFS en el período lluvioso (962 y 735 kg/ha que en el seco (505 y 378 kg/ha, respectivamente. La capacidad de carga en los métodos con labranza mínima se estimó en 2.5 UA/ha (5 vacas en el período de lluvias y de 1.2 y 1.4 U.A/ha (4 vacas en el período seco. En los métodos sin labranza mínima fue de 0.1 y 2 U.A/ha (1 y 4 vacas en el período de lluvias y de 0.1 y 0.4 U.A/ha (1 vaca en el período seco. ABSTRACT Renovation of pastures degraded the kikuyo pennisetum clandestinum, Hoechst, with minimum tillage in an andean high region de Colombia I. Productivity forrajera. Three methods of renewal of degraded pastures were used (Kikuyo without minimum tillage of pasture, KSLM; Kikuyo with minimum tillage, KLM and Kikuyo with minimum tillage more the application of chemical fertilization and clovers seeds, KLMFS, in rainy period and dry off; as a control it was included the traditional method of forage management in the farm (T. The availability of the green forage, was bigger in KLM and KLMFS in the rainy period (962 and 735 kg/ha that in the dry period (505 and 378 kg/ha. The load capacity in the methods with minimum farm you estimates in 2.5 UA/ha (5 cows in the period of rains and of 1.2 and 1.4 U.A/ha (4 cows in the dry period. In the methods without minimum farm was of 0.1 and 2 U.A/ha (1 and 4 cows in the period of rains and of 0.1 and 0.4 U.A/ha (1 cow in the dry period. Key words: Kikuyo grass, Pennisetum clandestinum Hoechst, pasture renewal, minimum tillage, Hills, Forage productivity, Cattle Use, High Andean Región.

Satellite tagging and biopsy sampling of killer whales at subantarctic Marion Island: effectiveness, immediate reactions and long-term responses.

Directory of Open Access Journals (Sweden)

Ryan R Reisinger

Full Text Available Remote tissue biopsy sampling and satellite tagging are becoming widely used in large marine vertebrate studies because they allow the collection of a diverse suite of otherwise difficult-to-obtain data which are critical in understanding the ecology of these species and to their conservation and management. Researchers must carefully consider their methods not only from an animal welfare perspective, but also to ensure the scientific rigour and validity of their results. We report methods for shore-based, remote biopsy sampling and satellite tagging of killer whales Orcinus orca at Subantarctic Marion Island. The performance of these methods is critically assessed using 1 the attachment duration of low-impact minimally percutaneous satellite tags; 2 the immediate behavioural reactions of animals to biopsy sampling and satellite tagging; 3 the effect of researcher experience on biopsy sampling and satellite tagging; and 4 the mid- (1 month and long- (24 month term behavioural consequences. To study mid- and long-term behavioural changes we used multievent capture-recapture models that accommodate imperfect detection and individual heterogeneity. We made 72 biopsy sampling attempts (resulting in 32 tissue samples and 37 satellite tagging attempts (deploying 19 tags. Biopsy sampling success rates were low (43%, but tagging rates were high with improved tag designs (86%. The improved tags remained attached for 26±14 days (mean ± SD. Individuals most often showed no reaction when attempts missed (66% and a slight reaction-defined as a slight flinch, slight shake, short acceleration, or immediate dive-when hit (54%. Severe immediate reactions were never observed. Hit or miss and age-sex class were important predictors of the reaction, but the method (tag or biopsy was unimportant. Multievent trap-dependence modelling revealed considerable variation in individual sighting patterns; however, there were no significant mid- or long-term changes

JPRS Report, Epidemiology.

Science.gov (United States)

1989-07-19

24 JPRS-TEP-89-013 19 JULY 1989 3 FEDERAL REPUBLIC OF GERMANY Bayer, Hoechst Test HIV Vaccine [Bonn TECHNOLOGIE NACHRICHTEN -MANAGEMENT...Hoechst Test HIV Vaccine MI89016 Bonn TECHNOLOGIE NACHRICHTEN - MANAGEMENTINFORMATIONEN in German 21 Feh 89 pp 16-17 [Excerpt] The Bayer and Hoechst

Cellular uptake, nuclear localization and cytotoxicity of 125I-labelled DNA minor groove binding ligands in K562, human erythroleukaemia cells

International Nuclear Information System (INIS)

Karagiannis, T.C.; Lobachevsky, P.N.; Martin, R.F.

2000-01-01

Full text: Iodine-125 decays by orbital electron capture and internal conversion resulting in the emission of numerous Auger electrons which produce a highly localised radiochemical damage in the immediate vicinity of the site of decay. Given the requirement to deliver 125 I to the nuclear DNA, a minor groove binding bibenzimidazole, 125 I-iodoHoechst 33258 was investigated. It has been noted that this analogue may be prone to de-iodination in vitro and in vivo, given the presence of an orthoiodophenol moiety which is analogous to that in thyroxins. Therefore, an 125 I -iodoHoechst analogue without the hydroxyl group was also studied. The 125 I -iodoHoechst 33258 analogue was prepared by direct iodination of Hoechst 33258 and 125 I iodoHoechst was prepared by demetallation of a trimethylstannyl precursor. DNA binding studies indicated that both iodo-analogues bind to calf thymus DNA, K D = 89 ± 30nM, n = 0.018 bp - 1 for iodoHoechst 33258 and K D = 121 ± 31nM, n = 0.024 bp -1 for iodoHoechst. Similarly, nuclear localization following incubation with 5μM of either ligand at 37 deg C was observed in K562 cells by fluorescence microscopy. Flow cytometry was used to investigate the kinetics of drug uptake and efflux in K562 cells. The results indicated that when 10 6 cells were incubated with 5μM ligand at 37 deg C, the uptake reached a plateau at approximately 43 minutes for iodoHoechst 33258 and approximately 52 minutes for iodoHoechst. Ligand efflux results indicated two-phase kinetics. The initial phase which involves 50-60% of drug was characterised by a half-life time (t 1/2 ) of 55.4 minutes for efflux of iodoHoechst 33258 and a t 1/2 of 10.3 minutes for efflux of iodoHoechst, at 37 deg C. Furthermore, the results suggested that the DNA binding sites in a 10 6 cell/ml suspension were saturated by incubation with 3μM iodoHoechst 33258 and 5μM iodoHoechst. In the initial cytotoxicity experiments using 125 I-iodoHoechst 33258, K562 cells were incubated for 1

Hydrogeology, water quality, and potential for contamination of the Upper Floridan aquifer in the Silver Springs ground-water basin, central Marion County, Florida

Science.gov (United States)

Phelps, G.G.

1994-01-01

The Upper Floridan aquifer, composed of a thick sequence of very porous limestone and dolomite, is the principal source of water supply in the Silver Springs ground-water basin of central Marion County, Florida. The karstic nature of the local geology makes the aquifer susceptible to contaminants from the land surface. Contaminants can enter the aquifer by seepage through surficial deposits and through sinkholes and drainage wells. Potential contaminants include agricultural chemicals, landfill leachates and petroleum products from leaking storage tanks and accidental spills. More than 560 sites of potential contamination sources were identified in the basin in 1990. Detailed investigation of four sites were used to define hydrologic conditions at representative sites. Ground-water flow velocities determined from dye trace studies ranged from about 1 foot per hour under natural flow conditions to about 10 feet per hour under pumping conditions, which is considerably higher than velocities estimated using Darcy's equation for steady-state flow in a porous medium. Water entering the aquifer through drainage wells contained bacteria, elevated concentrations of nutrients, manganese and zinc, and in places, low concentrations of organic compounds. On the basis of results from the sampling of 34 wells in 1989 and 1990, and from the sampling of water entering the Upper Floridan aquifer through drainage wells, there has been no widespread degradation of water quality in the study area. In an area of karst, particularly one in which fracture flow is significant, evaluating the effects from contaminants is difficult and special care is required when interpolating hydrogeologic data from regional studies to a specific. (USGS)

Radiobiology with DNA ligands

International Nuclear Information System (INIS)

Weinreich, R.; Argentini, M.; Guenther, I.; Koziorowski, J.; Larsson, B.; Nievergelt-Egido, M.C.; Salt, C.; Wyer, L.; Dos Santos, D.F.; Hansen, H.J.

1997-01-01

The paper deals with the following topics: labelling of DNA ligands and other tumour-affinic compounds with 4.15-d 124 I, radiotoxicity of Hoechst 33258 and 33342 and of iodinated Hoechst 33258 in cell cultures, preparation of 76 Br-, 123 I-, and 221 At-labelled 5-halo-2'-deoxyuridine, chemical syntheses of boron derivatives of Hoechst 33258.III., Gadolinium neutron capture therapy

Changes in Plasma Corticosterone and Catecholamine Contents Induced by Low Doses of Deltamethrin in Rats

NARCIS (Netherlands)

Boer, Sietse F. de; Gugten, Jan van der; Slangen, Jef. L.; Hijzen, Theo H.

1988-01-01

The effects of low doses of (S)-α-cyano-3-phenoxybenzyl (1R)-cis-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylate (Roussel UCLAF, Paris, France), (deltamethrin) upon sympathetic-adrenomedullary and pituitary-adrenocortical activity were investigated in rats by measuring plasma noradrenaline

Book Review: Current Issues in International Human Resource Management and Strategy Research

DEFF Research Database (Denmark)

Gretzinger, Susanne

2009-01-01

The article reviews the book "Current Issues in International Human Resource Management and Strategy Research," edited by Marion Festing and Susanne Royer.......The article reviews the book "Current Issues in International Human Resource Management and Strategy Research," edited by Marion Festing and Susanne Royer....

Structure-Based Design of CSDK4-Specific Inhibitors

National Research Council Canada - National Science Library

Marmorstein, Ronen

2003-01-01

...) are implicated in more than 80% of human neoplasias (Ortega et al., 2002). For example, the gene encoding the CDK4/6 inhibitory protein, p16INK4, is deleted or mutated in the majority of leukemias, bladder cancers and familial melanomas (Roussel, 1999...

40 CFR 81.341 - South Carolina.

Science.gov (United States)

2010-07-01

... County X Laurens County X Lee County X Lexington County X McCormick County X Marion County X Marlboro... X McCormick County X Marion County X Marlboro County X Newberry County X Oconee County X Orangeburg... County McCormick County Newberry County Oconee County Orangeburg County Pickens County Richland County...

The Feasibility of Educating Trainee Science Teachers in Issues of Science and Religion

Science.gov (United States)

Poole, Michael

2016-01-01

This article reflects on Roussel De Carvalho's paper "Science initial teacher education and superdiversity: educating science teachers for a multi-religious and globalized science classroom" (EJ1102211). It then offers suggestions for making some of the ambitious goals of the science-and-religion components of the science initial teacher…

Big Food, Big Agra, and the Research University

Science.gov (United States)

Warren, Cat

2010-01-01

This article presents an interview with Marion Nestle, the New York University food scientist and author of "Food Politics." Marion Nestle is Paulette Goddard Professor in the Department of Nutrition, Food Studies, and Public Health at New York University. From 1986 to 1988, she was senior nutrition policy adviser in the Department of Health and…

Author Details

African Journals Online (AJOL)

Killer whales (Orcinus orca) at Marion Island, Southern Ocean Abstract · Vol 37, No 1 (2002) - Articles Pup growth and maternal attendance patterns in Subantarctic fur seals. Abstract · Vol 37, No 1 (2002) - Articles Juvenile survival and population regulation in southern elephant seals at Marion Island Abstract · Vol 43, No 2 ...

Poet Marion Approval

Science.gov (United States)

This update August 9, 2016 letter from EPA approves, with modifications, the petition from Poet Biorefining-North Manchester, LLC, regarding non-grandfathered ethanol produced through a dry mill process, qualifying under the Clean Air Act for renewable

Research

African Journals Online (AJOL)

abp

24 juin 2015 ... Page number not for citation purposes. 1. Epaisseur de l'intima-média carotidienne et facteurs de risque cardio-vasculaires. Nicolas Fanantenana Herinirina1, Lova Hasina Ny Ony Narindra Rajaonarison2,&, Andry Roussel Herijoelison3, Ahmad Ahmad2. 1Service Imagerie Médicale, Centre Hospitalier ...

The feasibility of educating trainee science teachers in issues of science and religion

Science.gov (United States)

Poole, Michael

2016-06-01

This article reflects on Roussel De Carvalho's paper `Science initial teacher education and superdiversity: educating science teachers for a multi-religious and globalized science classroom'. It then offers suggestions for making some of the ambitious goals of the science-and-religion components of the science initial teacher education project more manageable.

Cooperation of the site and public fire and safety services in case of an incident

International Nuclear Information System (INIS)

Bauch, M.

1997-01-01

This report describes the cooperation between the site and the public fire and safety services in case of an incident. As an example, the measures and facilities of the Hoechst site of the Hoechst AG and the organisational and technical background are presented. (orig.) [de

Ways to Prepare Future Teachers to Teach Science in Multicultural Classrooms

Science.gov (United States)

Billingsley, Berry

2016-01-01

Roussel De Carvalho uses the notion of superdiversity to draw attention to some of the pedagogical implications of teaching science in multicultural schools in cosmopolitan cities such as London. De Carvalho makes the case that if superdiverse classrooms exist then Science Initial Teacher Education has a role to play in helping future science…

Thymidine analogues to assess microperfusion in human tumors

International Nuclear Information System (INIS)

Janssen, Hilde L.; Ljungkvist, Anna S.; Rijken, Paul F.; Sprong, Debbie; Bussink, Jan; Kogel, Albert J. van der; Haustermans, Karin M.; Begg, Adrian C.

2005-01-01

Purpose: To validate the use of the thymidine analogues as local perfusion markers in human tumors (no labeling indicates no perfusion) by comparison with the well-characterized perfusion marker Hoechst 33342. Methods and Materials: Human tumor xenografts from gliomas and head-and-neck cancers were injected with iododeoxyuridine (IdUrd) or bromodeoxyuridine (BrdUrd) and the fluorescent dye Hoechst 33342. In frozen sections, each blood vessel was scored for the presence of IdUrd/BrdUrd labeling and Hoechst in surrounding cells. The percentage of analogue-negative vessels was compared with the fraction of Hoechst-negative vessels. Collocalization of the two markers was also scored. Results: We found considerable intertumor variation in the fraction of perfused vessels, measured by analogue labeling, both in the human tumor xenografts and in a series of tumor biopsies from head-and-neck cancer patients. There was a significant correlation between the Hoechst-negative and IdUrd/BrdUrd-negative vessels in the xenografts (r 85, p = 0.0004), despite some mismatches on a per-vessel basis. Conclusions: Thymidine analogues can be successfully used to rank tumors according to their fraction of perfused vessels. Whether this fraction correlates with the extent of acute hypoxia needs further confirmation

The Aplacophora: History, Taxonomy, Phylogeny, Biogeography, and Ecology

Science.gov (United States)

1992-02-01

the Woods Hole Oceanographic Forschungsprojektes Polvm~de. Bulletin du Museum national Institution. d* Histoire naturelle.3 ;,s4r., 447: 413-421. Sal...British Columbia), Australian CSIRO (Hobart, Tasmania), and Departamento de Zoologia, Rio de Janeiro. Approved for Distribution: r.PtrH., Web :,Chsirman...A. F. Marion. 1882. Itudes sur les Neomenia. Zool. Anz. 5:61-64. Kowalevsky, A. and A. F. Marion. 1887. Contributions i 1’ histoire des Solenogastres

BASEMAP, MARION COUNTY, MS

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — FEMA Framework Basemap datasets comprise six of the seven FGDC themes of geospatial data that are used by most GIS applications (Note: the seventh framework theme,...

Materials Properties Data Management--Approaches to a Critical National Need

Science.gov (United States)

1983-09-01

Kanoui, R. Pasero, and P. Roussel. 1973. Un Systbme de Communication Home-machine en Francais. Rapport, Groupe Intelligence Artificielle . Luminy, France... intelligence ." This is a new and rapidly expanding field in which commercial markets have only started to develop (Verity 1982). It is worth noting...permitting the computer to perform automatic deductive reasoning. The application of such "artificial intelligence " will be discussed later. When

Perspectivas sociais e dominação simbólica: a presença política das mulheres entre Iris Marion Young e Pierre Bourdieu

Directory of Open Access Journals (Sweden)

Luis Felipe Miguel

2010-06-01

Full Text Available O artigo trata da participação política das mulheres e de sua presença no poder político. Nessa temática, discute a tensão entre o potencial emancipacionista prometido pela incorporação de múltiplas perspectivas ao debate político e a ação reprodutora do campo. Partindo das causas que, a nosso ver, são responsáveis pela relativa ausência de mulheres dos círculos decisórios e por seu "desinteresse" pela política, discutimos as perspectivas orientadas, de uma forma ou de outra, para a solução (melhoramento dessa situação. O artigo organiza-se em três seções. Na primeira, defende-se a posição de que a via mais promissora para justificar a necessidade de presença das mulheres passa pelo entendimento de que os espaços de deliberação devem abrigar uma pluralidade de perspectivas sociais relevantes - um conceito associado, sobretudo, à obra da teórica estadunidense Iris Marion Young. Na segunda, discute-se alguns problemas desse conceito, em especial, certa ingenuidade que marca um ideal dele derivado: a geração de um espaço plural de discussão e de tomada de decisão em função da adoção de cotas eleitorais. Utiliza-se o conceito de "campo", extraído da obra de Pierre Bourdieu, para depurar as idéias de Young dessa ingenuidade. Na terceira seção, introduz-se um elemento adicional: a distinção, apresentada por Nancy Fraser, entre "políticas afirmativas" e "políticas transformadoras". Conclui-se, de forma preliminar, com um balanço dos limites e das potencialidades de uma política baseada na defesa da ampliação da presença de "perspectivas sociais".

Wittgenstein, finitism, and the foundations of mathematics

CERN Document Server

Marion, Mathieu

2008-01-01

Mathieu Marion traces the development of Wittgenstein''s thinking from the 1920s through to the 1950s, in the context of the mathematical and philosophical work of the time, making sense of ideas that have often been misunderstood. He shows that study of Wittgenstein''s writings on mathematics is essential to a proper understanding of his philosophy. - ;Mathieu Marion offers a careful, historically informed study of Wittgenstein''s philosophy of mathematics. This area of his work has frequently been undervalued by Wittgenstein specialists and by philosophers of mathematics alike; but the surprising fact that he wrote more on this subject than on any other indicates its centrality in his thought. Marion traces the development of Wittgenstein''s thinking in the context of the mathematical and philosophical work of the times, to make. coherent sense of ideas that have too often been misunderstood because they have been presented in a disjointed and incomplete way. In particular, he illuminates the work of the ne...

Logic Programming in LISP.

Science.gov (United States)

1981-01-01

Rapport, Groupe Intelligence Pasero, R., Artificielle , Universite d’Aix-Marseille, Roussel, P. Luminy, France, 1973. [Kowalski 1974] Kowalski, R. A...THIS PAGZ(Whan Doee Es tMord) Item 20 (Cont’d) ------ work in the area of artificial intelligence and those used in general program development into a...logic programming with LISP for implementing intelligent data base query systems. Continued developments will allow for enhancements to be made to the

Viral Immunotherapy to Eradicate Subclinical Brain Metastases

Science.gov (United States)

2014-05-01

flash frozen brain tissue. Hoechst and CFSE labeled cells are readily visualized in fresh CSF. The brightest staining is achieved with Hoechst and is...viral infection in the meninges is in part due to reduction of the effects of suppressor macrophages. The work is complicated by the fact that...therapeutic effect of viral infection in the meninges is in part due to reduction of the effects of suppressor macrophages. • We found that mice cured

Spatial relationship between tumor perfusion and endogeneous glucose distribution

International Nuclear Information System (INIS)

Schroeder, T.; Larrier, N.; Viglianti, B.; Rabbani, Z.N.; Peltz, C.; Vujascovic, Z.; Dewhirst, M.W.

2003-01-01

Earlier studies detecting glucose in tissue and solid tumors by bioluminescence imaging suggested, that glucose distribution patterns may be spatially related to functional vascularity. The purpose of this study was to evaluate this relationship by comparing glucose distribution patterns as determined by bioluminescence imaging to perfusion patterns of endogeneous Hoechst 33342 in rats bearing mammary carcinomas. R 3230 mammary carcinoma cells have been implanted subcutaneously into 7 female Fischer 344 rats. Two months post implantation, after injection of Hoechst 33342 the tumors were removed and snap frozen to conserve metabolite levels. Concomitantly, blood was sampled from the animals for analysis of glucose concentrations using a micodialysis analyzer. Cryosections of the tumors have been prepared, and every slice has been analyzed for both, Hoechst binding by fluorescence microscopy, and for glucose distribution patterns using bioluminescence imaging. In many cases vascular structures could be retrieved by the spatial pattern of glucose distribution. In some cases however, higher glucose concentrations could be found independent from Hoechst signal. On the other hand, regions of high Hoechst signal are not necessarily correlated with high glucose concentrations. When comparing blood and tissue glucose levels, tissue glucose content as measured with bioluminescence imaging (1.9-3.5 mM) is considerably lower than blood glucose (5.6-8.0 mM), demonstrating the expected gradient from blood to tissue. This study demonstrates the feasibility of monitoring glucose gradients in relation to functional vasculature throughout the body, from blood down to tissue or tumor and further, throughout the microenvironment of the solid tumor. Glucose distribution patterns may be an important tool in perfusion studies, e. g. in detecting the direction of blood flow in ex-vivo samples or in estimating glucose consumption rates of tumor cells adjacent to or in between perfused

Novel Combinatory Approaches to Repair Visual System after Optic Nerve Damage

Science.gov (United States)

2014-09-01

Novel Combinatory Approaches to Repair Visual System After Optic Nerve Damage 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6...biological functions such as metabolism, growth, cell cycle, and survival ( Cardone et al., 1998; Diehl, Cheng, Roussel, & Sherr, 1998; Hers, Vincent...of injured adult sensory neurons. The Journal of Neuroscience, 21, 7161–7170. Cardone , M. H., Roy, N., Stennicke, H. R., Salvesen, G. S., Franke, T. F

Hunger for carbon dioxide. Foundation of the 'Task Force Biomass' for the development of new energy sources; Hunger nach Kohlendioxid. 'Task Force Biomasse' zur Erschliessung neuer Energiequellen gegruendet

Energy Technology Data Exchange (ETDEWEB)

Schlaefer, Susanne; Bayer, Thomas [Infraserv GmbH und Co. Hoechst KG, Frankfurt am Main (Germany)

2009-10-15

At the industrial park Hoechst (Frankfurt / Main, Federal Republic of Germany), Infraserv GmbH and Co. Hoechst KG pursues the disposal conception ''Waste to Energy'' in order to supply electricity and steam for the approximately 90 producing companies at the location. The company now looks for possibilities in order to develop new energy sources. The keyword is ''innovative biomass''. Among other things the possibility of power generation from algae belongs to this.

Targeting Extracellular DNA to Deliver IGF-1 to the Injured Heart

Science.gov (United States)

Khan, Raffay S.; Martinez, Mario D.; Sy, Jay C.; Pendergrass, Karl D.; Che, Pao-Lin; Brown, Milton E.; Cabigas, E. Bernadette; Dasari, Madhuri; Murthy, Niren; Davis, Michael E.

2014-03-01

There is a great need for the development of therapeutic strategies that can target biomolecules to damaged myocardium. Necrosis of myocardium during a myocardial infarction (MI) is characterized by extracellular release of DNA, which can serve as a potential target for ischemic tissue. Hoechst, a histological stain that binds to double-stranded DNA can be conjugated to a variety of molecules. Insulin-like growth factor-1 (IGF-1), a small protein/polypeptide with a short circulating-half life is cardioprotective following MI but its clinical use is limited by poor delivery, as intra-myocardial injections have poor retention and chronic systemic presence has adverse side effects. Here, we present a novel delivery vehicle for IGF-1, via its conjugation to Hoechst for targeting infarcted tissue. Using a mouse model of ischemia-reperfusion, we demonstrate that intravenous delivery of Hoechst-IGF-1 results in activation of Akt, a downstream target of IGF-1 and protects from cardiac fibrosis and dysfunction following MI.

Marion L. Williams Interview (MORS)

OpenAIRE

Williams, Marion L.

2015-01-01

Interviewers: Keethler, Greg; Sheldon, Robert S.. Interview location(s): Headquarters Air Force Operational Test and Evaluation Center, Kirkland Air Force Base, New Mexico and United States Air Force Academy, Colorado Springs, Colorado

Summary of high field diffusion MRI and microscopy data demonstrate microstructural aberration in chronic mild stress rat brain

DEFF Research Database (Denmark)

Khan, Ahmad Raza; Chuhutin, Andrey; Wiborg, Ove

2016-01-01

amygdala of the same brain hemispheres is also included with three different stains: DiI and Hoechst stained microscopic images (confocal microscopy) andALDH1L1 antibody based immunohistochemistry.These stains may be used to evaluate neurite density (DiI), nuclear density (Hoechst) and astrocytic density...... (ALDH1L1). This combination of high field diffusion data and high resolution images from microscopy enables comparison of microstructural parameters derived from diffusion MRIto histological microstructure. The data provided here is used in the article (Jespersen, 2016) [1]....

A Study on Strategy Establishment for Foreign Market Penetrating of Nuclear Technology Venture Business

Energy Technology Data Exchange (ETDEWEB)

Song, Ki Dong; Kim, S. S.; Choi, Y. D.; Park, C. S.; Bae, C. I.; Chung, J. M.; Roe, S. P

2007-05-15

The objective of this study is to suggest feasible strategy for foreign market penetration of nuclear venture technology business. Especially, this study focuses on the technology and product export to the Frankfurt-Hoechst Industrial Park in Germany. This study analyzed the operating system of German Industrial Park, especially the Frankfurt-Hoechst Industrial Park. This study also analyzed the current status of Industrial Science Parks in Northern European countries such as Finland, Sweden, and England. Then, this study suggested the strategy for nuclear technology venture business to penetrate German export market effectively.

A Study on Strategy Establishment for Foreign Market Penetrating of Nuclear Technology Venture Business

International Nuclear Information System (INIS)

Song, Ki Dong; Kim, S. S.; Choi, Y. D.; Park, C. S.; Bae, C. I.; Chung, J. M.; Roe, S. P.

2007-05-01

The objective of this study is to suggest feasible strategy for foreign market penetration of nuclear venture technology business. Especially, this study focuses on the technology and product export to the Frankfurt-Hoechst Industrial Park in Germany. This study analyzed the operating system of German Industrial Park, especially the Frankfurt-Hoechst Industrial Park. This study also analyzed the current status of Industrial Science Parks in Northern European countries such as Finland, Sweden, and England. Then, this study suggested the strategy for nuclear technology venture business to penetrate German export market effectively

Photosensitization by iodinated DNA minor groove binding ligands: Evaluation of DNA double-strand break induction and repair.

Science.gov (United States)

Briggs, Benjamin; Ververis, Katherine; Rodd, Annabelle L; Foong, Laura J L; Silva, Fernando M Da; Karagiannis, Tom C

2011-05-03

Iodinated DNA minor groove binding bibenzimidazoles represent a unique class of UVA photosensitizer and their extreme photopotency has been previously characterized. Earlier studies have included a comparison of three isomers, referred to as ortho-, meta- and para-iodoHoechst, which differ only in the location of the iodine substituent in the phenyl ring of the bibenzimidazole. DNA breakage and clonogenic survival studies in human erythroleukemic K562 cells have highlighted the higher photo-efficiency of the ortho-isomer (subsequently designated UV(A)Sens) compared to the meta- and para-isomers. In this study, the aim was to compare the induction and repair of DNA double-strand breaks induced by the three isomers in K562 cells. Further, we examined the effects of the prototypical broad-spectrum histone deacetylase inhibitor, Trichostatin A, on ortho-iodoHoechst/UVA-induced double-strand breaks in K562 cells. Using γH2AX as a molecular marker of the DNA lesions, our findings indicate a disparity in the induction and particularly, in the repair kinetics of double-strand breaks for the three isomers. The accumulation of γH2AX foci induced by the meta- and para-isomers returned to background levels within 24 and 48 h, respectively; the number of γH2AX foci induced by ortho-iodoHoechst remained elevated even after incubation for 96 h post-irradiation. These findings provide further evidence that the extreme photopotency of ortho-iodoHoechst is due to not only to the high quantum yield of dehalogenation, but also to the severity of the DNA lesions which are not readily repaired. Finally, our findings which indicate that Trichostatin A has a remarkable potentiating effect on ortho-iodoHoechst/UVA-induced DNA lesions are encouraging, particularly in the context of cutaneous T-cell lymphoma, for which a histone deacetylase inhibitor is already approved for therapy. This finding prompts further evaluation of the potential of combination therapies. Copyright © 2011

Mati Undi teater revisited / Luule Epner

Index Scriptorium Estoniae

Epner, Luule, 1953-

2009-01-01

Teatrilavastuste taastamisest üldisemalt. Pikemalt festivali UNT! programmis etendunud Mati Undi lavastustest "Majahoidja", "Vaimude tund Jannseni tänaval", "Helene, Marion ja Felix", originaali ja taastatud variandi võrdlus

Assessing microscope image focus quality with deep learning.

Science.gov (United States)

Yang, Samuel J; Berndl, Marc; Michael Ando, D; Barch, Mariya; Narayanaswamy, Arunachalam; Christiansen, Eric; Hoyer, Stephan; Roat, Chris; Hung, Jane; Rueden, Curtis T; Shankar, Asim; Finkbeiner, Steven; Nelson, Philip

2018-03-15

Large image datasets acquired on automated microscopes typically have some fraction of low quality, out-of-focus images, despite the use of hardware autofocus systems. Identification of these images using automated image analysis with high accuracy is important for obtaining a clean, unbiased image dataset. Complicating this task is the fact that image focus quality is only well-defined in foreground regions of images, and as a result, most previous approaches only enable a computation of the relative difference in quality between two or more images, rather than an absolute measure of quality. We present a deep neural network model capable of predicting an absolute measure of image focus on a single image in isolation, without any user-specified parameters. The model operates at the image-patch level, and also outputs a measure of prediction certainty, enabling interpretable predictions. The model was trained on only 384 in-focus Hoechst (nuclei) stain images of U2OS cells, which were synthetically defocused to one of 11 absolute defocus levels during training. The trained model can generalize on previously unseen real Hoechst stain images, identifying the absolute image focus to within one defocus level (approximately 3 pixel blur diameter difference) with 95% accuracy. On a simpler binary in/out-of-focus classification task, the trained model outperforms previous approaches on both Hoechst and Phalloidin (actin) stain images (F-scores of 0.89 and 0.86, respectively over 0.84 and 0.83), despite only having been presented Hoechst stain images during training. Lastly, we observe qualitatively that the model generalizes to two additional stains, Hoechst and Tubulin, of an unseen cell type (Human MCF-7) acquired on a different instrument. Our deep neural network enables classification of out-of-focus microscope images with both higher accuracy and greater precision than previous approaches via interpretable patch-level focus and certainty predictions. The use of

Renovación de pasturas degradadas de kikuyo, Pennisetum clandestinum Hoechst, con labranza mínima en una región alto andina de Colombia II. Productividad animal

Directory of Open Access Journals (Sweden)

García Indira Isis

2005-03-01

Full Text Available Se utilizaron vacas lactantes de doble propósito, en pasturas de kikuyo sometidas a tres métodos de renovación (kikuyo sin labranza mínima, KSLM; kikuyo con labranza mínima, KLM; y kikuyo con labranza mínima más la aplicación de fertilización química y siembra de tréboles, KLMFS en período lluvioso o seco; se incluyó como testigo (T el manejo en la finca. La producción de leche no presentó diferencias entre métodos con labranza mínima y sin ella, mientras que la producción en kg/ha-día fue mayor en KLMFS y KLM, en el período lluvioso (43 y 40 kg y con KSLM y T (31.2 y 8 kg. En el período seco la producción continuó siendo mayor con KLMFS y KLM (28 y 25.2 kg y KSLM y T (6.6 y 6.4 kg respectivamente. La calidad de la leche (porcentaje de grasa en el período de lluvias fue baja para todos los métodos. La condición corporal de las vacas en el período lluvioso se mantuvo estable (3.0; en el período seco fue mayor en pasturas con labranza mínima (2.8. La eficiencia reproductiva fue mejor en pasturas con labranza mínima (IEP de 12.2 meses. ABSTRACT Renovation of pastures degrades de kikuyo Pennisetum Clandestinum, Hoechst, with minimum farm in areas of hillsides in an andean high región de Colombia. II. Animal productivity. Cows lactantings of double purpose were used in kikuyo pastures, subjected to three methods of renovation (Kikuyo Without Minimum Farm, KSLM; Kikuyo with Minimum Farm, KLM and kikuyo with Minimum Farm more the application of chemical fertilization and siembra of clovers, KLMFS. in dry and rainy periods; it was included as control (C, the traditional handling pasture in the property. The production of milk in kg/vaca-day, it don’t present differences among methods with and without minimum farm. While the production in kg/ha-day was bigger in KLMFS and KLM, in the rainy period (43 and 40 kg and with KSLM and C (31.2 and 8 kg. In the dry period, the production continued being bigger with

Workshop on Diffuse Discharge Opening Switches (January 13-15, 1982).

Science.gov (United States)

1982-04-23

published). 6. E. H. A. Granneman, M. Klewer, K. J. Nygaard and M. J. Van der Wiel, "Two-photon ionization of Cs2 , Rb2 and RBCs using an Ar-ion laser...G. H. Bearman and J. J. Leventhal, Phys. Rev. Lett 41, 1227 (1978). 3. A. deJong and F. van der Volk, J. Phys. B 12, L561 (1979). 225 4. F. Roussel, P...Marshall Molen Old Dominion University R.M. Patrick AVCO Everett Research Laboratory Karl Schoenbach Texas Tech University Peter Turchi R & D Associates

Compartmental modelling of the pharmacokinetics of a breast cancer resistance protein.

Science.gov (United States)

Grandjean, Thomas R B; Chappell, Mike J; Yates, James T W; Jones, Kevin; Wood, Gemma; Coleman, Tanya

2011-11-01

A mathematical model for the pharmacokinetics of Hoechst 33342 following administration into a culture medium containing a population of transfected cells (HEK293 hBCRP) with a potent breast cancer resistance protein inhibitor, Fumitremorgin C (FTC), present is described. FTC is reported to almost completely annul resistance mediated by BCRP in vitro. This non-linear compartmental model has seven macroscopic sub-units, with 14 rate parameters. It describes the relationship between the concentration of Hoechst 33342 and FTC, initially spiked in the medium, and the observed change in fluorescence due to Hoechst 33342 binding to DNA. Structural identifiability analysis has been performed using two methods, one based on the similarity transformation/exhaustive modelling approach and the other based on the differential algebra approach. The analyses demonstrated that all models derived are uniquely identifiable for the experiments/observations available. A kinetic modelling software package, namely FACSIMILE (MPCA Software, UK), was used for parameter fitting and to obtain numerical solutions for the system equations. Model fits gave very good agreement with in vitro data provided by AstraZeneca across a variety of experimental scenarios. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

76 FR 40930 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

Science.gov (United States)

2011-07-12

... COLUMBIA District of Columbia Saint Paul African Union Methodist Church, 401 I St. SE., Washington... Marion Historic District (Boundary Increase), W. Cherry, E. Main, N. Main, Maple, N. Chestnut, Broad & N...

Marion Island and Prince Edward Island

African Journals Online (AJOL)

spamer

that nest above the surface of the ground. They include .... searched on each of the surveys is shown in Figure 2c .... tribution of nests in 1997/98 is shown in Figure 2b. The ...... RIDOUX, V. 1994 — The diets and dietary segregation of seabirds.

Floodplain Mapping for Marion County, SC

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

Iris Marion Young and Political Education

Science.gov (United States)

Frazer, Elizabeth

2006-01-01

This paper will focus on Young's theories of heterogeneity, as they have developed from the essays in "Throwing Like a Girl" (1990) to those in "Inclusion and Democracy" (2000). Reading her theories of heterogeneity together with recent developments in political theory which seek to reclaim the agonistic and frictional aspects of polity and…

DCS Terrain for Marion County, GA

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix M: Data Capture Standards, describes the digital topographic data that was used to create...

Väike pahe või suur kirg? / Katrin Pauts

Index Scriptorium Estoniae

Pauts, Katrin, 1977-

2006-01-01

Veinijoomisest, sigari suitsetamisest ning kaartide tagumisest räägivad sigarisalongi La Casa del Habano perenaine Angela Leima, Tartu Veiniklubi liige Katrin Pärgmäe ja bridzhiklubi president Marion Ründal

Why calories count: from science to politics

National Research Council Canada - National Science Library

Nestle, Marion; Nesheim, Malden C

2012-01-01

.... They are also hard to understand. In Why Calories Count, Marion Nestle and Malden Nesheim explain in clear and accessible language what calories are and how they work, both biologically and politically...

Interactions Between Mantle Plumes and Mid-Ocean Ridges: Constraints from Geophysics, Geochemistry, and Geodynamical Modeling

National Research Council Canada - National Science Library

Georgen, Jennifer

2001-01-01

This thesis studies interactions between mid-ocean ridges and mantle plumes. Chapter 1 investigates the effects of the Marion and Bouvet hotspots on the ultra-slow spreading, highly-segmented Southwest Indian Ridge (SWIR...

Cloud amount/frequency, NITRATE and other data from CAPRICORNE, KOROTNEFF and other platforms from 1957-05-14 to 1977-07-21 (NODC Accession 8000153)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Water depth and other Ocean Station data collected from multiple ships such as CAPRICORNE, CORIOLIS, JEAN CHARCOT, KOROTNEF, LE NOROIT and MARION DUFRESNE from May...

76 FR 60748 - Changes in Flood Elevation Determinations

Science.gov (United States)

2011-09-30

... respective addresses are listed in the table below. FOR FURTHER INFORMATION CONTACT: Luis Rodriguez, Chief...: Linn City of Marion (11-07- August 11, 2011; The Honorable Paul Rehn, December 16, 2011 190191 1284P...

Kanada invasioon pakub üllatusi / Peeter Riba

Index Scriptorium Estoniae

Riba, Peeter, 1967-

2003-01-01

PÖFFil linastuvaid Kanada mängufilme - Richard Kwietniowski "Mängur Mahowny" ("Owning Mahowny"), Denys Arcand'i "Barbarite sissetung" ("Les Invasions barbares"), Wiebke von Carolsfeldi "Marion Bridge" ja Thom Fitzgeraldi "Üritus" ("The Event")

Pulse radiolysis studies on DNA-Binding radioprotectors

International Nuclear Information System (INIS)

Anderson, R.F.

1996-01-01

Full text: Hoechst 33342 and newly-synthesised analogues exhibit radioprotective activity in cultured cells and in vivo, as described in accompanying abstracts. These minor groove binding ligands bind at discreet sites in DNA, characterised by 3 to 4 consecutive AT base pairs, and DNA sequencing studies have shown focussed radioprotection at these binding sites. There is evidence that the bound ligands also confer more 'global' protection including the intervening DNA between the binding sites. The observed focussed radioprotection could be explained by H-atom donation from the ligand to radiation-induced carbon-centred deoxyribosyl radicals, but this mechanism is unlikely to account for the global radioprotection. We now report pulse radiolysis studies on another possible mechanism, namely reduction of transient radiation-induced oxidising species on DNA by the ligand, which is consistent with the report of reduction of G + by TMPD. Oxidation of deoxyguanosine (dG) by Br 2 - , produced by radiolysis of Br- in N 2 0-saturated solutions, in the presence of Hoechst 33342 results in the appearance of a transient ligand species which is kinetically resolvable from that obtained from direct oxidation of Hoechst 33342 by Br 2 - . A plot of reaction rate versus ligand concentration indicates that the rate constant for reduction of G + is approximately 3 x 10 8 dm 3 M -1 sec -1 . Similar experiments with DNA, rather than dG, also revealed a transient species corresponding to oxidation of the ligand, but the absolute rate of oxidation was considerably slower for the DNA-bound ligand compared to that for oxidation of the free ligand by G+. These results are clearly consistent with the proposed mechanism of radioprotection by Hoechst 33342 and its analogues, moreover, pulse radiolysis may provide a very useful endpoint for screening new analogues, as a preliminary to radiobiological evaluation

The Information Manager Vol.8 (1)2008 Page 27

African Journals Online (AJOL)

Gbaje E.S

in her budgets for information and information technology and also open up government business to public scrutiny, required in .... stressed the importance of information management by pointing ... both internal and external sources.” Marion ...

HORMONE LEVELS IN THE PERIPHERAL BLOOD OF THE ...

African Journals Online (AJOL)

, 1972. Plasma oestrogen and progesterone levels after mating, and during late pregnancy and post partum in cows . Endocrinologt, 90, 1336. MARION, G.B. & GIER, H.T., 1968. Factors affecting bovine ovarian activity after parturition. J. Anim.

2013 Oregon Department of Geology and Mineral Industries (DOGAMI) Oregon Lidar: Clackamol

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — WSI collected Light Detection and Ranging (LiDAR) data for the Oregon LiDAR Consortium (OLC) Clackamol 2013 study area in Clackamas and Marion County, Oregon. The...

2013 Oregon Department of Geology and Mineral Industries (DOGAMI) Oregon Lidar DEM: Clackamol

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — WSI collected Light Detection and Ranging (LiDAR) data for the Oregon LiDAR Consortium (OLC) Clackamol 2013 study area in Clackamas and Marion County, Oregon. The...

Análise argumentativa do léxico: o exemplo da palavra 'medo'

Directory of Open Access Journals (Sweden)

Carel, Marion

2009-01-01

Full Text Available Neste artigo, Marion Carel apresenta uma análise argumentativa da palavra medo fundamentada na Teoria dos Blocos Semânticos, momento anual da Semântica Argumentativa, desenvolvida por Carel e Ducrot

Detecting EUV transients in near real time with ALEXIS

Energy Technology Data Exchange (ETDEWEB)

Roussel-Dupre`, D.; Bloch, J.J.; Theiler, J.; Pfafman, T.; Beauchesne, B.

1995-12-31

The Array of Low Energy X-ray Imaging Sensors (ALEXIS) experiment consists of a mini-satellite containing six wide angle EUV/ultrasoft X-ray telescopes (Priedhorsky et al. 1989, and Bloch et al. 1994). Its scientific objective is to map out the sky in three narrow ({Delta}E/E {approx} 5%) bandpasses around 66, 71, and 93 eV. During each 50 second satellite rotation period the six telescopes, each with a 30{degrees} field, of:view and a spatial resolution of 0.25{degrees}, scan most of the antisolar hemisphere of the sky. The project is a collaborative effort between Los Alamos National Laboratory, Sandia National Laboratory, and the University of California-Berkeley Space Sciences Laboratory. It is controlled entirely from a small ground station located at Los Alamos. The mission was launched on a Pegasus Air Launched Vehicle on April 25, 1993. An incident at launch delayed our ability to properly analyze the data until November of 1994. In January of 1995, we brought on line automated software to routinely carry out the transient search. After the data is downlinked from the satellite, the software processes and transforms it into sky maps that are automatically searched for new sources. The software then sends the results of these searches by e-mail to the science team within two hours of the downlink. This system has successfully detected the Cataclysmic Variables VW Hyi, U Gem and AR UMa in outburst, and has detected at least two unidentified short duration EUV transients (Roussel-Dupre et al 1995, Roussel-Dupre 1995).

Dependence of FDG uptake on tumor microenvironment

International Nuclear Information System (INIS)

Pugachev, Andrei; Ruan, Shutian; Carlin, Sean; Larson, Steven M.; Campa, Jose; Ling, C. Clifton; Humm, John L.

2005-01-01

Purpose: To investigate the factors affecting the 18 F-fluorodeoxyglucose ( 18 F-FDG) uptake in tumors at a microscopic level, by correlating it with tumor hypoxia, cellular proliferation, and blood perfusion. Methods and Materials: Nude mice bearing Dunning prostate tumors (R3327-AT) were injected with 18 F-FDG and pimonidazole, bromodeoxyuridine, and, 1 min before sacrifice, with Hoechst 33342. Selected tumor sections were imaged by phosphor plate autoradiography, while adjacent sections were used to obtain the images of the spatial distribution of Hoechst 33342, pimonidazole, and bromodeoxyuridine. The images were co-registered and analyzed on a pixel-by-pixel basis. Results: Statistical analysis of the data obtained from these tumors demonstrated that 18 F-FDG uptake was positively correlated with pimonidazole staining intensity in each data set studied. Correlation of FDG uptake with bromodeoxyuridine staining intensity was always negative. In addition, FDG uptake was always negatively correlated with the staining intensity of Hoechst 33342. Conclusions: For the Dunning prostate tumors studied, FDG uptake was always positively correlated with hypoxia and negatively correlated with both cellular proliferation and blood flow. Therefore, for the tumor model studied, higher FDG uptake is indicative of tumor hypoxia, but neither blood flow nor cellular proliferation

Frequency Characteristics of Geomagnetic Induction Anomalies in ...

Indian Academy of Sciences (India)

19

Western continental margin of India (WCMI) has been affected by (a) Marion hotspot .... Onshore region consist of NW-SE trending basin between Jamnagar and ..... potential of deep sea basins; 7th offshore south Asia conference, Singapore., ...

Population biology of house mice (Mus musculus L.) on sub ...

African Journals Online (AJOL)

1993-05-03

May 3, 1993 ... Studies on the feral house mouse Mus musculus in habitats ranging from deserts ... Previous studies on mice at Marion Island focused on ..... and food availability) may decrease the rate of development .... Wiley, New York.

Photosynthesis in a sub-Antarctic shore-zone lichen

NARCIS (Netherlands)

Smith, V.; Gremmen, N.J.M.

2001-01-01

Photosynthetic responses to moisture, light, temperature, salinity and inorganic nitrogen fertilization are reported for a shore-zone lichen Turgidiusculum complicatulum (formerly Mastodia tesselata), a possible recent introduction to sub-Antarctic Marion Island. Optimum moisture contents for net

Dandruff: How to Treat

Medline Plus

Full Text Available ... and Lectureship Marion B. Sulzberger Award and Lectureship Master Dermatologist Award Members Making a Difference Award Native ... Recognition Program Leadership Institute Programs Mentoring Leadership Learning Center Residents and Fellows Resource Center Join the AAD ...

How to Stop Biting Your Nails

Medline Plus

Full Text Available ... and Lectureship Marion B. Sulzberger Award and Lectureship Master Dermatologist Award Members Making a Difference Award Native ... Recognition Program Leadership Institute Programs Mentoring Leadership Learning Center Residents and Fellows Resource Center Join the AAD ...

Bug bites and stings: When to see a dermatologist

Medline Plus

Full Text Available ... and Lectureship Marion B. Sulzberger Award and Lectureship Master Dermatologist Award Members Making a Difference Award Native ... Recognition Program Leadership Institute Programs Mentoring Leadership Learning Center Residents and Fellows Resource Center Join the AAD ...

Urmas Sõõrumaa avas Kohilas näituse / Inge Põlma

Index Scriptorium Estoniae

Põlma, Inge

2009-01-01

Näitus Urmas Sõõrumaa kunstikogust pärit Eestimaaga seotud 19. sajandi kunstnike - Lorenz-Heinrich Petersen, Wilhelm Siegfried Stavenhagen, Eugen Gustav Dücker - graafikast, akvarellidest ja maalidest Kohila vallamajas 21. aprillist 22. juunini. Kuraator Marion Tupits

Dandruff: How to Treat

Medline Plus

Full Text Available ... and Lectureship Marion B. Sulzberger Award and Lectureship Master Dermatologist Award Members Making a Difference Award Native ... Fee schedule Managing a practice Prior authorization assistance Evaluating practice models Teledermatology Compliance HIT Webinars Media relations ...

How to Stop Biting Your Nails

Medline Plus

Full Text Available ... and Lectureship Marion B. Sulzberger Award and Lectureship Master Dermatologist Award Members Making a Difference Award Native ... Fee schedule Managing a practice Prior authorization assistance Evaluating practice models Teledermatology Compliance HIT Webinars Media relations ...

Reviews of recent publications

Directory of Open Access Journals (Sweden)

various authors

1995-01-01

Full Text Available Elisabeth Bronfen. Over Her Dead Body: Death, Feminity and the Aesthetic by Lisa Maruca Jacques Dupin: Selected Poems . (Translated by Paul Auster, Stephen Romer, and David Shipiro. by Maryann De Julio Carolyn A. Durham. The Contexture of Feminism: Marie Cardinal and Multicultural Literacy by Yolanda Astarita Patterson Mike Gonzalez and David Treece. The Gathering of Voices: The Twentieth-Century Poetry of Latin America by Steven F. White Emily D. Hicks. Border Writing: The Multidimensional Text by Roselyn Constantino Robin Régine. Socialist Realism: An Impossible Aesthetic by Peter Hitchcock Kari Weil. Androgyny and the Denial of Difference by Brigitte Roussel

The subantarctic Prince Edward Islands are globally important ...

African Journals Online (AJOL)

spamer

(Vulnerable) has increased significantly, making Prince Edward Island equal with Marion Island as supporting ... 3 Marine & Coastal Management, Department of Environmental Affairs and Tourism, ... breeding on inaccessible cliff areas were estimated by ...... Penguin Conservation Assessment and Management Plan.

How to Stop Biting Your Nails

Medline Plus

Full Text Available ... and Lectureship Clarence S. Livingood Award and Lectureship Marion B. Sulzberger Award and Lectureship Master Dermatologist Award Members ... care Nail care Nail biting "); (function () { var a = "", b = [ "adid=aad-aad-1", "site=ehs.con.aad. ...

Bug bites and stings: When to see a dermatologist

Medline Plus

Full Text Available ... and Lectureship Clarence S. Livingood Award and Lectureship Marion B. Sulzberger Award and Lectureship Master Dermatologist Award Members ... skin Bug bites and stings "); (function () { var a = "", b = [ "adid=aad-aad-1", "site=ehs.con.aad. ...

I Remember Highlander

Science.gov (United States)

Williams-Hawkins, Maria

2016-01-01

"I Remember Highlander" reflects on the life choices of Marion Barry and Herman Henning Jr., fraternity brothers who sought the same goal but took different paths. The essay examines cultural and family situations that shaped lives and decisions.

Hamilton and Zuk 1982, Earlé et al. 1991, Bennet et al. 1995, Jovani

African Journals Online (AJOL)

spamer

hatching (Graczyk et al. 1994). ... Its success has been attributed to the ... Future success of avian blood parasites and/or ... rates (Trager 1939, Roberts 1968), but this does not happen ... The insects of sub-Antarctic Marion and Prince Edward.

Off With Their Heads! A Serio-Comic Tale of Hollywood.

Science.gov (United States)

Marion, Frances

Frances Marion was one of Hollywood's leading screenplay writers from the silent era through the 1940's. This informal memoir concentrates on the personalities she knew, the development of major motion pictures, her friends, and her own career. (JK)

Salvianolic Acid-A Induces Apoptosis, Mitochondrial Membrane ...

African Journals Online (AJOL)

using Hoechst 33258 staining. The effect of the compound on mitochondrial membrane potential loss ... Fluorescence microscopy demonstrated that salvianolic acid-A induced dose- dependent ..... aggregation and anticancer properties. It has.

Quenching of nucleotide-derived radicals by bisbenzimidazole ...

Indian Academy of Sciences (India)

Unknown

aRadiation Chemistry and Chemical Dynamics Division, Bhabha Atomic ... reaction of DNA-minor-groove ligand bisbenzimidazole Hoechst 33258 with ... flights, nuclear industries and management of radiation-induced accidents 1,2.

The uranium and thorium separation in the chemical reprocessing of the irradiated fuel of thorium and uranium mixed oxides

International Nuclear Information System (INIS)

Oliveira, E.F. de.

1984-09-01

A bibliographic research has been carried out for reprocessing techniques of irradiated thorium fuel from nuclear reactors. The Thorex/Hoechst process has been specially considered to establish a method for reprocessing thorium-uranium fuel from PWR. After a series of cold tests performed in laboratory it was possible to set the behavior of several parameters affecting the Thorex/Hoechst process. Some comments and suggestions are presented for modifications in the process flosheet conditions. A discussion is carried out for operational conditions such as the aqueous to organic flow ratio the acidity of strip and scrub solutions in the process steps for thorium and uranium recovery. The operation diagrams have been constructed using equilibrium experimental data which correspond to conditions observed in laboratory. (Author) [pt

The gentoo penguin Pygoscelis papua is one of four species of ...

African Journals Online (AJOL)

spamer

This makes the assumption that birds did not nest at more than one ... on this Near Threatened species at Marion Island must take full account of its susceptibility to human disturbance. ... mated for the colonies at Trypot Beach, Macaroni Bay,.

Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni

Directory of Open Access Journals (Sweden)

AK Sarvel

2006-10-01

Full Text Available Schistosoma mansoni eggs are classified, according to morphological characteristics, as follows: viable mature and immature eggs; dead mature and immature eggs, shells and granulomas. The scope of this study was to compare the staining characteristics of different morphological types of eggs in the presence of fluorescent labels and vital dyes, aiming at differentiating live and dead eggs. The eggs were obtained from the intestines of infected mice, and put into saline 0.85%. The fluorescent labels were Hoechst 33258 and Acridine Orange + Ethidium Bromide and vital dyes (Trypan Blue 0.4% and Neutral Red 1%. When labelled with the probe Hoechst 33258, some immature eggs, morphologically considered viable, presented fluorescence (a staining characteristic detected only in dead eggs; mature eggs did not present fluorescence, and the other types of dead eggs, morphologically defined, showed fluorescence. As far as Acridine Orange + Ethidium Bromide are concerned, either the eggs considered to be live, or the dead ones, presented staining with green color, and only the hatched and motionless miracidium was stained with an orange color. Trypan Blue was not able to stain the eggs, considered to be dead but only dead miracidia which had emerged out of the shell. Neutral Red stained both live and dead eggs. Only the fluorescent Hoechst 33258 can be considered a useful tool for differentiation between dead and live eggs.

78 FR 43854 - Designation for the Muncie, IN; Fremont, NE; Annapolis, MD; and West Lafayette, IN Areas...

Science.gov (United States)

2013-07-22

..., Hendricks, Henry, Jay, Johnson, Madison, Marion, Monroe, Morgan, Randolph, Rush, Shelby, Union, and Wayne Counties. In Ohio Darke County. This designation action to provide official services in these specified... Cooperative elevator located in Shelby, Polk County, Nebraska. Correction In the Federal Register Notice...

Alguses oli Piaf / Kaisa Karu

Index Scriptorium Estoniae

Karu, Kaisa

2010-01-01

Režissöör Christopher Nolani põnevusfilmis "Algus" (USA-Inglise 2010) teeb kaasa ka prantsuse näitleja Marion Cotillard. Tuntuks sai näitlejanna peaosaga režissöör Olivier Dahani filmis "Edith Piaf - elusse armunud"

A Future Outlook for Women Executives.

Science.gov (United States)

1988-04-01

revive Kentucky Fried Chicken . Maryann Schneider, 40; Beneficial Corp., Wilmington, Del.; senior vice-president for planning and administration; KS...Marion 0. Sandler -- co-chairman of the Golden West Financial Corporation, which has $34 billion dollars in assets, and Wendy Luscombe -- President of Pan

African Zoology - Vol 29, No 2 (1994)

African Journals Online (AJOL)

Population biology of house mice (Mus musculus L.) on sub-Antarctic Marion Island · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. D.C. Matthewson, R.J. van Aarde, J.D. Skinner, 99-106 ...

76 FR 10253 - Final Flood Elevation Determinations

Science.gov (United States)

2011-02-24

... Martin Luther King Jr. Boulevard, Room 310, Evansville, IN 47708. Marion County, Kansas, and Incorporated... at the Evansville Civic Center Complex, Building Commission Department, 1 Northwest Martin Luther King Jr. Boulevard, Room 310, Evansville, IN 47708. Unincorporated Areas of Vanderburgh County Maps are...

FLOODPLAIN, MARION COUNTY, OHIO, AND INCORPORATED AREAS

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

Ya no es necesario hacer obras con ayuda de la tecnología; mejor hacer máquinas que hagan las obras por nosotros. César Aira y los procedimientos de escritura.

Directory of Open Access Journals (Sweden)

Juan Camilo Rodriguez Pira

2016-10-01

Full Text Available Resumen:La obra de César Aira es una reflexión sobre el proceso creativo. Las máquinas y procedimientos no solo son metáforas de ello; más que novelas, se proponen formas de novelar: procedimientos que, operando como máquinas, combinan elementos variopintos. Siguiendo los pasos de Raymond Roussel, al elegir un procedimiento el escritor se libera de sí mismo y de sus propias invenciones. Con premisas afines a las de Marcel Duchamp, las novelas de Aira se presentan como ‘máquinas solteras’: artefactos inútiles e intrincados, únicos e irrepetibles. El papel del artista es crear máquinas que hagan las obras por él. El lector recibe más que obras abiertas: las novelas son manuales de instrucciones.AbstractCésar Aira’s oeuvre is a constant reflection on the creative process. Machines and procedures are not only seen as metaphors that deal with this process; the very texts are not simply novels, but rather ways of composing novels: they design procedures that work like machines in order to combine different elements and produce new works. Following the steps of Raymond Roussel, if artists use a mechanical procedure, it will free them from their own inventions and personality; thus, chance and machinations allow the artist to discover something new. Following premises close to the ones of Marcel Duchamp, César Aira’s novels are presented as machines célibataires: useless, intricate and unique artifacts. Artists’ role is to create machines that do the work for them. The reader, then, faces more than an open work: these novels are instruction manuals.

Electrochemical detection of specific DNA and respiratory activity of Escherichia coli

International Nuclear Information System (INIS)

Yamanaka, Keiichiro; Ikeuchi, Tomohiko; Saito, Masato; Nagatani, Naoki; Tamiya, Eiichi

2012-01-01

We present two rapid and simplified detection methods for Escherichia coli involving the use of a hand-held potentiostat and a disposable screen-printed carbon electrode. E. coli is one of the indicator organisms used to access for food safety. Commonly, microbiological culture techniques take more than one day to yield results and therefore, a simple, cost-effective, in situ detection system is required for testing food safety. This report describes two complementary techniques for high- and low-sensitivity detection of E. coli. High-sensitivity detection relies upon quantification of DNA amplification by using polymerase chain reaction (PCR), while the simplified, low-sensitivity detection can be obtained through measurement of oxygen consumption due to respiration; importantly, both techniques utilize the same type of electrode. The former entails mixing the PCR mixture with Hoechst, an electro-active DNA intercalator, and then, measuring the oxidation current. Binding of Hoechst molecules to the amplified DNA causes the peak current to decrease because of the slow diffusion of the Hoechst-amplified DNA complex to the electrode surface. The results showed that the oxidation peak current of Hoechst decreased depending on the number of E. coli cells added to the PCR mixture as the template for amplification, and the sensitivity of the method was as low as a single bacterium. Oxygen consumption was detected by direct measurement of the cell-containing culture medium. This method required only 10 μL to be applied on the screen-printed electrode, and the reduction in oxygen current was clearly observed within 30 min when a minimum of 1 × 10 5 cells were present. These results were obtained without purifying the culture, and the samples were applied onto the electrode without any surface modifications. The techniques describes in this report are versatile, because they require the same type of electrode, have simplistic nature, use a hand-held potentiostat, and have

Comparison of commercial diagnostic kits for placental lactogen radioimmunoassay

International Nuclear Information System (INIS)

Pirkl, S.; Kulenda, Z.

1976-01-01

The biochemical laboratory compared the following diagnostic kits for HPL determination: Pharmacia (Sweden), Radiochemical Center Amersham (GB), NEN (USA), Hoechst (FRG), applying the following criteria: 1. price, 2. number of analyses for which one kit may be applied, 3. expiration time, 4. speed, i.e., time elapsed from sample delivery to report (dependent on incubation time), 5. laboriousness, 6. reproducibility, 7. compatibility of values obtained by different kits. Alcohol precipitation (Pharmacia, RCC Amersham) appeared to be the most suitable while Dextran coated charcoal (NEN) and Amberlite (Hoechst) were somewhat more complicated, and the Sorin double antibody technique (ultrafiltration and washing of precipitate on filtres was used for separation) appeared to be the most laborious technique. RCC Amersham was ranked first owing to its lower cost as compared with runner-up Pharmacia. (L.O.)

To Innovate Better, Find Divergent Thinkers

DEFF Research Database (Denmark)

Poetz, Marion; Hippel, Eric von

2015-01-01

The article discusses the practice of seeking advice and ideas from practitioners in analogous fields. It references a study on search methodologies by Marion Poetz and colleagues at the Copenhagen Business School and Zeppelin University. Poetz and colleagues used a pyramid search methodology dev...

The macaroni penguin Eudyptes chrysolophus has a subantarctic ...

African Journals Online (AJOL)

spamer

Key words: breeding, diet, Eudyptes chrysolophus, macaroni penguin, Marion Island, population, Subantarctic ... Sea Elephant Bay. Van den Boogaard River. Ship's Cove (south). Macaroni Bay (north). Archway Bay (north). East Cape (a). Bullard Beach North (a) ... of breeding birds at these two colonies were mapped.

South African red data book – Birds

CSIR Research Space (South Africa)

Brooke, RK

1984-12-01

Full Text Available Antarctic Tern Kerguelen Tern. All are included because of feral cat Felis catus predation on Marion Island. Only the Common Diving Petrel and the Kerguelen Tern do not visit South African coastal waters. It is also instructive to examine the 102...

Melt, fluid and crystal inclusions in olivine phenocrysts from Kerguelen plume-derived picritic basalts: evidence for interaction with the Kerguelen Plateau lithosphere.

NARCIS (Netherlands)

Borisova, A.U.; Nikogosian, I.; Shimizu, N.; Weis, D.; Scoates, J.S.; Touret, J.L.R.; Damasceno, D.

2002-01-01

Melt, fluid and crystal inclusions have been studied in olivine phenocrysts from a suite of picritic basalts dredged during the "Marion Dufresne" MD 109 cruise (Dredge 6) from a seamount located between the Kerguelen Archipelago and Heard Island (Southern Indian Ocean). A two-stage polybaric

76 FR 53927 - Kansas; Major Disaster and Related Determinations

Science.gov (United States)

2011-08-30

... determinations. DATES: Effective Date: July 29, 2011. FOR FURTHER INFORMATION CONTACT: Peggy Miller, Office of... Public Assistance and Hazard Mitigation will be limited to 75 percent of the total eligible costs...: Barton, Clay, Cloud, Hamilton, Jewell, Lincoln, Logan, Lyon, Marion, Mitchell, Morton, Osage, Osborne...

76 FR 48831 - Arbitration Panel Decision Under the Randolph-Sheppard Act

Science.gov (United States)

2011-08-09

... to manage the Marion County vending route comprised of vending machines at the Oregon Department of... cafeteria was selling items in competition with Complainant's vending machines. On October 4, 2006... vendor complained of unexpected low earnings soon after he began operating the vending machines. Thus...

40 CFR 81.349 - West Virginia.

Science.gov (United States)

2010-07-01

... Marshall County Mason County McDowell County Mercer County Mineral County Mingo County Monongalia County... Jefferson County Lewis County Lincoln County Logan County Marion County Marshall County Mason County... County Mason County McDowell County Mercer County Mineral County Mingo County Monongalia County Monroe...

Continuous spins in 2D gravity: Chiral vertex operators and local fields

International Nuclear Information System (INIS)

Gervais, Jean-Loup; Schnittger, Jens

1994-01-01

We construct the exponentials of the Liouville field with continuous powers within the operator approach. Their chiral decomposition is realized using the explicit Coulomb-gas operators we introduced earlier. From the quantum group viewpoint, they are related to semi-infinite highest- or lowest-weight representations with continuous spins. The Liouville field itself is defined, and the canonical commutation relations are verified, as well as the validity of the quantum Liouville field equations. In a second part, both screening charges are considered. The braiding of the chiral components is derived and shown to agree with an ansatz of a parallel paper of Gervais and Roussel. ((orig.))

Protective Effect of Modified Human Acidic Fibroblast Growth Factor ...

African Journals Online (AJOL)

HP

Methods: NRK52E cell apoptotic death was measured by several methods including cell morphologic observation, Hoechst ... In addition, the levels of phosphorylated-Akt protein were ... of serum creatinine and urea nitrogen, and enhance the ...

New records of Acari from the sub-Antarctic Prince Edward Islands

NARCIS (Netherlands)

Marshall, D.J.; Gremmen, N.J.M.; Coetzee, L.; Oconnor, B.M.; Pugh, P.J.A.; Theron, P.D.; Ueckermann, E.A.

1999-01-01

Sixty species of Acari are recorded from the sub-Antarctic Marion and Prince Edward Islands (the Prince Edward archipelago). Twenty of the 45 species collected on recent expeditions are new and currently undescribed. Other new taxa include a family of Mesostigmata, four new genera, and the first

Congruent, decreasing trends of gentoo penguins and Crozet shags ...

African Journals Online (AJOL)

Numbers of gentoo penguins Pygoscelis papua and Crozet shags Phalacrocorax [atriceps] melanogenis breeding annually at Marion Island, one of South Africa's Prince Edward Islands in the South-West Indian Ocean, were strongly correlated over 19 split-years from 1994/1995 to 2012/2013. Both species decreased ...

Profiles in Organizational Effectiveness for Nonprofits: Improving the Lives of Children, Youth and Families in Kansas City's Urban Core.

Science.gov (United States)

Ewing Marion Kauffman Foundation, Kansas City, MO.

For some time the Ewing Marion Kauffman Foundation has been studying the attributes that characterize effective nonprofit organizations. These attributes were identified after a review of the literature, discussions with national leaders, meetings with administrators and funders of nonprofit organizations, and the information from case studies by…

Kivirähk, Pinter ja Inglise spioonid Rakvere teatris / Kristo Kruusman

Index Scriptorium Estoniae

Kruusman, Kristo

2010-01-01

Tänavu 70. sünnipäeva tähistava teatri repertuaaris on mitu uuslavastust: Andrus Kivirähk "Kaelkirjak", lavastaja Marion Undusk, Patrick Barlow’ spioonikomöödia „39 astet”, lavastaja Kati Kivitar ja Harold Pinteri näidend „Eikellegimaa”, lavastajaks Peeter Raudsepp

The Marine Mammal Programme at the Prince Edward Islands: 38 ...

African Journals Online (AJOL)

The Marine Mammal Programme (MMP) conducts research on pinnipeds and killer whales Orcinus orca at Marion Island, Prince Edward Islands, under the auspices of the Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria. The history of the MMP, which has benefited from ...

76 FR 72196 - CRD Hydroelectric LLC; Western Minnesota Municipal Power Agency; Notice of Application for...

Science.gov (United States)

2011-11-22

... Hydroelectric LLC; Western Minnesota Municipal Power Agency; Notice of Application for Transfer of License, and Soliciting Comments and Motions To Intervene On October 14, 2011, CRD Hydroelectric LLC (transferor) and... Red Rock Hydroelectric Project, No. 12576, located on the Des Monies River in Marion County, Iowa...

A method for the generation of random multiple Coulomb scattering angles

International Nuclear Information System (INIS)

Campbell, J.R.

1995-06-01

A method for the random generation of spatial angles drawn from non-Gaussian multiple Coulomb scattering distributions is presented. The method employs direct numerical inversion of cumulative probability distributions computed from the universal non-Gaussian angular distributions of Marion and Zimmerman. (author). 12 refs., 3 figs

Timber resource statistics for non-federal forest land in northwest Oregon.

Science.gov (United States)

Donald R. Gedney; Patricia M. Bassett; Mary A. Mei

1986-01-01

This report summarizes a 1986 timber resource inventory of the non-Federal forest land in the 10 counties (Clackamas, Clatsop, Columbia, Hood River, Marion, Multnomah , Polk, Tillamook, Washington, and Yamhill) in northwest Oregon. Detailed tables of forest area, timber volume, growth, mortality, and harvest are presented.

A rapid fluorometric method for semiautomated determination of cytotoxicity and cellular proliferation of human tumor cell lines in microculture.

Science.gov (United States)

Larsson, R; Nygren, P

1989-01-01

A fluorometric method for the determination of cellular growth and cytotoxicity of human tumor cell lines in 96-well microculture plates is described. The assay is based on the combined use of the DNA-binding dye Hoechst 33342 and the fluorogenic substrate fluorescein diacetate (FDA). Hoechst 33342 undergoes a dramatic enhancement of fluorescence when specifically intercalated with cellular DNA, whereas the FDA fluorescence is dependent on cellular hydrolysis of the non-fluorescent substrate into its fluorescent product. Fluorescence from both dyes was linearly related to the density of freshly seeded cells (6 x 10(3)-1 x 10(5)/well) and correlated well with physical cell count of cells under normal culture conditions as well as in response to the vinca alkaloid vincristine. However, the amount of FDA fluorescence produces and retained by the cultures was clearly dependent on the fraction of intact and viable cells, whereas the fluorescence reported by Hoechst 33342 was not. The assay was found to be simple, reliable and many samples could be analysed in a short period of time with minimal waste of cells and biological reagents. Apart from giving an estimate of cell density, the protocol described also provides a separate index of viability which in certain situations may be of importance for distinguishing between cytocidal and cytostatic drug actions. The method may be well suited for several applications, including the large scale screening for antitumor activity of compounds with potential cytocidal or cytostatic actions.

Non-electrostatic complexes with DNA: towards novel synthetic gene delivery systems.

Science.gov (United States)

Soto, J; Bessodes, M; Pitard, B; Mailhe, P; Scherman, D; Byk, G

2000-05-01

We have developed new DNA complexing amphiphile based on Hoechst 33258 interaction with DNA grooves. The synthesis and physicochemical characterisation of lipid/DNA complexes, as compared to that of classical lipopolyamine for gene delivery, are described and discussed.

Using a Gravity Model to Predict Circulation in a Public Library System.

Science.gov (United States)

Ottensmann, John R.

1995-01-01

Describes the development of a gravity model based upon principles of spatial interaction to predict the circulation of libraries in the Indianapolis-Marion County Public Library (Indiana). The model effectively predicted past circulation figures and was tested by predicting future library circulation, particularly for a new branch library.…

Recent trends in numbers of Crozet shags breeding at the Prince ...

African Journals Online (AJOL)

There were similar trends in numbers breeding and breeding success of gentoo penguins Pygoscelis papua, which at Marion Island have a similar diet to Crozet shags, suggesting that both species may have been influenced by food availability. Numbers of Crozet shags breeding at Prince Edward Island approximately ...

Southwest Energy Innovation Forum: Summary Report

Science.gov (United States)

Ewing Marion Kauffman Foundation, 2010

2010-01-01

The Ewing Marion Kauffman Foundation, Arizona State University (ASU), and U.S. Department of Energy's Advanced Research Projects Agency-Energy (ARPA-E) co-convened a conference on Energy Innovation in the Southwest region of the United States that included participation by entrepreneurs, state government officials, representatives of academia,…

Briti filmiakadeemia nimetas parimaks "Lepituse"

Index Scriptorium Estoniae

2008-01-01

Briti filmiakdeemia auhinnad tehti teatavaks 10. veebruaril : parim film "Lepitus" (režii Joe Wright), parim režissöör Ethan ja Joel Coen ("Ei ole maad vanadele meestele"), parim meesnäitlelja Daniel Day-Lewis, naisnäitleja Marion Cotillard, parim muukeelne film "Teiste elu" (Saksamaa)

Training the Twenty-First Century Special Forces Warrior: Does Character Matter When Training the Adaptive Leader

Science.gov (United States)

2006-06-16

whale that caused the ship to sink, forcing Marion and three others to take refuge in one of the lifeboats (Cummings 2005, 1). He spent a week on...disorder (2004, 4). The post-traumatic stress disorder then often spurs other addictive behaviors, increasing the inability to regain normalcy among

40 CFR 81.318 - Kentucky.

Science.gov (United States)

2010-07-01

... County X Mason County X Meade County X Menifee County X Mercer County X Metcalfe County X Monroe County X... X Magoffin County X Marion County X Marshall County X Martin County X Mason County X Meade County X... Marshall County Unclassifiable/Attainment Martin County Unclassifiable/Attainment Mason County...

Writing in Nonstandard English. Pragmatics & Beyond New Series 67.

Science.gov (United States)

Taavitsainen, Irma, Ed.; Melchers, Gunnel, Ed.; Pahta, Paivi, Ed.

Chapters in this book include the following: "Dickens as Sociolinguist: Dialect in 'David Copperfield'" (Patricia Poussa); "Contemporary Irish Writing and a Model of Speech Realism (John M. Kirk); "Dialect and Accent in Jim Cartwright's Play "Road" as Seen Through Erving Goffman's Theory on Footing" (Marion Fields); "The Representation of…

Cytotoxicity of Essential Oil of Chenopodium ambrosioides L against ...

African Journals Online (AJOL)

Hoechst33258 staining and DNA ladder indicate that the essential oil induced apoptosis. SOD vitality ... Whole plant of C. ambrosioides was collected from Chengdu in ..... The role of free radicals in toxicity and disease. Journal of basic and ...

Use of simple sequence repeat (SSR) markers for screening blue ...

African Journals Online (AJOL)

Aghomotsegin

2015-10-14

Oct 14, 2015 ... 4Ukiriguru Agricultural Research Institute, P. O. Box 1433 Mwanza, Tanzania. Received 25 May ... blue disease in Tanzania may potentially be due to the .... using Hoechst dye and the quality of the DNA samples was checked ...

The effect of cat Felis catus predation on three breeding ...

African Journals Online (AJOL)

Breeding success of Pterodroma macroptera, Procellaria aequinoctialis and Pachyptila vittata salvini in three cat-free and three control areas were used to evaluate the effects of cat Felis catus predation on the avifauna of Marion Island. Breeding success of all three species was significantly higher in the combined cat-free ...

27 CFR 9.78 - Ohio River Valley.

Science.gov (United States)

2010-04-01

... Louisville map) to the town of New Marion in Ripley County, Indiana (Cincinnati map). (7) The boundary proceeds in a straight line northerly to the town of Clarksburg in Decatur County, Indiana (Cincinnati map). (8) The boundary proceeds in a straight line easterly to the town of Ridgeville in Warren County...

75 FR 58305 - Approval and Promulgation of Air Quality Implementation Plans; Indiana; Revised Format for...

Science.gov (United States)

2010-09-24

... monoxide, Hydrocarbons, Incorporation by reference, Intergovernmental relations, Lead, Nitrogen dioxide... 60735 Sec. 6. 7/31/2009 11/20/2009, 74 FR 60197 Sec. 3. 1-2 Definitions 9/26/1980 11/5/1981, 46 FR 54943.../2009, 74 FR 52891.... Paragraph (c). and Marion Cos. 2009 Chicago Hydrocarbon Control Strategy..... 8...

Fluctuations in the population of southern elephant seals Mirounga ...

African Journals Online (AJOL)

and seals (Laws 1973). Predation. Carrick and Ingham (1962) indicated that the killer whale. Orcinus orca is an important predator of elephant seals, es- pecially of immature individuals. Moreover, Condy (1977) suggests that the observed decrease in the elephant seal population at Marion Island is due to a lowered recruit-.

Prey and seasonal abundance of killer whales at sub-Antarctic ...

African Journals Online (AJOL)

The diet of killer whales Orcinus orca was investigated from 48 predation events observed during sightings at sub-Antarctic Marion Island between 2006 and 2009. From these events, there were 10 cases where prey could be identified. Killer whales fed on fur seals Arctocephalus tropicalis, elephant seals Mirounga leonina ...

Sajandilõpu müüdid ja muinasjutud / Krista Kaer

Index Scriptorium Estoniae

Kaer, Krista

1997-01-01

Ulmekirjandusest (science fiction) ja nn. fantaasiakirjandusest (fantasy). Ka Dan Simmonsi, Octavia E. Butleri, Ursula K. Le Guini, Kim Stanley Robinsoni, Iain M. Banksi, Stephen Baxteri, Sheri S. Tepperi, Ellen Daitlowi, Terry Windlingi, T. H. White'i, Robin Hobbi, Charles de Linti, Marion Zimmer Bradley, Terry Pratchetti, ja Douglas Adamsi loomingust

Expression of early transcription factors Oct-4, Sox-2 and Nanog by porcine umbilical cord (PUC matrix cells

Directory of Open Access Journals (Sweden)

Schultz Bruce

2006-02-01

Full Text Available Abstract Background Three transcription factors that are expressed at high levels in embryonic stem cells (ESCs are Nanog, Oct-4 and Sox-2. These transcription factors regulate the expression of other genes during development and are found at high levels in the pluripotent cells of the inner cell mass. The downregulation of these three transcription factors correlates with the loss of pluripotency and self-renewal, and the beginning of subsequent differentiation steps. The roles of Nanog, Oct-4 and Sox-2 have not been fully elucidated. They are important in embryonic development and maintenance of pluripotency in ESCs. We studied the expression of these transcription factors in porcine umbilical cord (PUC matrix cells. Methods Cells were isolated from Wharton's jelly of porcine umbilical cords (PUC and histochemically assayed for the presence of alkaline phosphatase and the presence of Nanog, Oct-4 and Sox-2 mRNA and protein. PCR amplicons were sequenced and compared with known sequences. The synthesis of Oct-4 and Nanog protein was analyzed using immunocytochemistry. FACS analysis was utilized to evaluate Hoechst 33342 dye-stained cells. Results PUC isolates were maintained in culture and formed colonies that express alkaline phosphatase. FACS analysis revealed a side population of Hoechst dye-excluding cells, the Hoechst exclusion was verapamil sensitive. Quantitative and non-quantitative RT-PCR reactions revealed expression of Nanog, Oct-4 and Sox-2 in day 15 embryonic discs, PUC cell isolates and porcine fibroblasts. Immunocytochemical analysis detected Nanog immunoreactivity in PUC cell nuclei, and faint labeling in fibroblasts. Oct-4 immunoreactivity was detected in the nuclei of some PUC cells, but not in fibroblasts. Conclusion Cells isolated from PUC express three transcription factors found in pluripotent stem cell markers both at the mRNA and protein level. The presence of these transcription factors, along with the other

Radiation sensitization by an iodine-labelled DNA ligand

Energy Technology Data Exchange (ETDEWEB)

Martin, R F; Murray, V; D' Cunha, G; Pardee, M; Haigh, A; Hodgson, G S [Peter MacCallum Cancer Inst., Melbourne (Australia); Kampouris, E; Kelly, D P [Melbourne Univ., Parkville (Australia)

1990-05-01

An iodinated DNA ligand, iodoHoechst 33258, which binds in the minor groove of DNA, enhances DNA strand breakage and cell killing by UV-A irradiation. The sites of UV-induced strand breaks reflect the known sequence specificity of the ligand. (author).

Additions and corrections to the lichen mycobiota of the subantarctic Prince Edward Islands

NARCIS (Netherlands)

Øvstedal, D.O.; Gremmen, N.J.M.

2007-01-01

Fifteen species are reported as new to the subantarctic Prince Edward Islands (47 degrees S, 38 degrees E). Of these 12 are added to the known lichens of Prince Edward Island, and four are added to the Marion Island species list. Two species are new to science, viz. Gyalecta azorellae Ovstedal, with

Finding Conjectures Using Geometer's Sketchpad

Science.gov (United States)

Fallstrom, Scott; Walter, Marion

2011-01-01

Conjectures, theorems, and problems in print often appear to come out of nowhere. Scott Fallstrom and Marion Walter describe how their thinking and conjectures evolved; they try to show how collaboration helped expand their ideas. By showing the results from working together, they hope readers will encourage collaboration amongst their students.…

Erotic Love

DEFF Research Database (Denmark)

Søltoft, Pia

2011-01-01

The claim of this article is that in Søren Kierkegaard's notion of love as a basic urge in every human being we find a special dialectic between lack and surplus, between need-love and gift-love. Through a comparison with Jean-Luc Marion's description of erotic love in The Erotic Phenomenon, I...

Applying the SWAT hydrologic model on a watershed containing forested karst.

Science.gov (United States)

Devendra M. Amatya; Amy E. Edwards

2009-01-01

The US Forest Service Center for Forested Wetlands Research is working on a South Carolina Department of Health and Environmental Control (SC DHEC)'s Section 319 Grant Program funded Total Maximum Daily Load (TMDL) project for the watershed of Chapel Branch Creek (CBC) draining to Lake Marion in Santee, South Carolina (Fig. 1)....

Production and cost of a live skyline cable yarder tested in Appalachia

Science.gov (United States)

Edward L. Fisher; Harry G. Gibson; Cleveland J. Biller

1980-01-01

Logging systems that are profitable and environmentally acceptable are needed in Appalachian hardwood forests. Small, mobile cable yarders show promise in meeting both economic and environmental objectives. One such yarder, the Ecologger, was tested on the Jefferson National Forest near Marion, Virginia. Production rates and costs are presented for the system along...

Mervyn Hine (1920-2004)

CERN Multimedia

2004-01-01

Dr. Mervyn Hine, CERN pioneer of the Proton Synchrotron construction, and of computing and networking, ex CERN Director, passed away yesterday 26 April, following a serious accident in his home. He died peacefully, surrounded by his wife Jenny, and his daughters Alison, Marion and Jessica. An obituary will appear in the next Bulletin.

Young Entrepreneurs: Young People Dream of Becoming Entrepreneurs, despite America's Lingering Recession

Science.gov (United States)

Ewing Marion Kauffman Foundation, 2011

2011-01-01

An August 2010 Harris Interactive[R] survey, commissioned by the Ewing Marion Kauffman Foundation, posed questions about entrepreneurship to 5,077 U.S. young people ages eight to twenty-four. The results show that business ownership continues to capture the imaginations of America's youth, particularly for those who know a successful entrepreneur…

School Attendance, Absenteeism, and Student Success. Research Brief

Science.gov (United States)

Oregon Department of Education, 2015

2015-01-01

Oregon Department of Education staff spoke with principals from five Oregon schools that had low rates of chronic absenteeism compared to schools with similar demographics during the 2014-15 school year: Echo Shaw Elementary School, Free Orchards Elementary School, Dayton Junior High School, Valor Middle School, and North Marion High School. Each…

40 CFR 52.773 - Approval status.

Science.gov (United States)

2010-07-01

... Clark, Elkhart, Floyd, Lake, Marion, Porter, and St. Joseph Counties satisfy all requirements of Part D... ozone plan for Lake and Porter Counties, which was required to be submitted by July 1, 1992, does not... the Clean Air Act, as amended in 1977: (1) The transportation control plans for Lake, Porter, Clark...

Long-term flow dynamics of three coastal experimental forested watersheds

Science.gov (United States)

Devendra M. Amatya; Artur Radecki-Pawlik

2005-01-01

Three 1st2nd, and 3rd order experimental forested watersheds located within Francis Marion National Forest in Coastal South Carolina were monitored for rainfall and stream outflows. These watersheds were WS80, a pine-hardwood forest (206 ha); WS79 a predominantly pine forest (500 ha); and WS78, a...

Selection of viable cell subpopulations from murine tumours using FACS

International Nuclear Information System (INIS)

Chaplin, D.J.; Durand, R.E.; Olive, P.L.

1985-01-01

The authors developed a technique which enables isolation of viable tumour cells subpopulation as a function of their distance from the blood supply. The basis for this separation procedure is that the fluorochrome, Hoechst 33342, as a result of its high avidity for cellular DNA, exhibits a marked diffusion/consumption gradient when it has to pass through several cell layers. As a result intravenous injection of Hoechst 33342 into tumour bearing animals, results in a heterogeneous straining pattern within the tumour with cells close to blood vessels being brightly fluorescent while those more distant are less intensely stained. Since these differences in staining intensity persist after tumour disaggregation, cells can be sorted into subpopulations on the basis of their fluorescence intensity using a fluorescence activated cell sorter. This technique offers the unique possibility of identifying the location of those cell subpopulations resistant to treatment with either radiation or chemotherapeutic drugs

Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage.

Science.gov (United States)

Huo, Li-Jun; Ma, Xing-Hong; Yang, Zeng-Ming

2002-10-15

The purpose of this study was to assess sperm quality in extended boar semen during in vitro storage in order to determine which extender should be used and how long boar semen can be stored. Freshly ejaculated boar semen was diluted with equal volumes of Beltsville thaw solution (BTS), Androhep, KIEV or Zorlesco extenders and stored at 17 degrees C for up to 15 days. Sperm quality was evaluated by examining viability using SYBR-14/PI and Hoechst 33258 staining, mitochondrial activity using 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) staining, acrosome intactness by Coomassie blue staining, and capacitation status by chlortetracycline (CTC) staining. There were over 50% viable spermatozoa in boar semen extended with Zorlesco and Androhep extenders on Day 13 of storage. The percentage of JC-1-stained spermatozoa was 53.8 +/- 2.1% for Zorlesco and 57.7 +/- 1.60% for Androhep extenders on Day 13 of storage. The percentage of acrosome-intact spermatozoa detected by Coomassie blue staining was higher than that in the SYBR-14PI-, Hoechst 33258-, and JC-1-stained samples in our study. The results from SYBR-14/PI, Hoechst 33258, JC-1, and Coomassie blue staining were highly correlated (r > or = 0.9461). There were less than 15% capacitated spermatozoa in the semen extended with BTS, Androhep and Zorlesco extenders during 9 days of storage. However, most viable boar spermatozoa became capacitated by Day 13 of storage. The rank order of four extenders for maintaining sperm viability and mitochondrial activity was as follows: Androhep, Zorlesco, BTS, KIEV.

The lichens of Marion and Prince Edward Islands

NARCIS (Netherlands)

Øvstedal, D.O.; Gremmen, N.J.M.

2001-01-01

A total of 100 lichen species are listed for the islands. Six species are described as new to science: Arctomia subantarctica Øvstedal, Arthothelium evanescens Øvstedal, Coccotrema stellata Øvstedal, Ramonia subantarctica Øvstedal, Thelocarpon subantarcticum Øvstedal and Verrucaria umbilicata

Uus projekt tõstab tarkvara kvaliteeti / Marion Lepmets

Index Scriptorium Estoniae

Lepmets, Marion

2006-01-01

Projekti ESPIN (Estonian Software Process Improvement Network) eesmärk on projektis osalevate tarkvarafirmade teenuste ja toodete kvaliteedi tõstmine kvaliteedialaste konsultatsioonide ja koolituste abil

Floodplain Mapping, Marion County, Kansas and Incorporated Areas

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

Freshwater invertebrates of sub-Antarctic Marion Island | Dartnall ...

African Journals Online (AJOL)

The aquatic species include five platyhelminthes, a gastrotrich, three tardigrades, 28 rotifers, six nematodes, two annelids and 11 arthropods. Most are familiar species that have been recorded on other sub-Antarctic islands. The invertebrate faunas of the various freshwater habitats were basically similar in species ...

Nigerian Veterinary Journal - Vol 36, No 2 (2015)

African Journals Online (AJOL)

Anthelmintic Efficacy Trials using Fractionsof Ethanolic Crude Extract of Anogeissusschimperi Hoechst against Nippostrongylus braziliensis in Rats · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. OC Jegede, MS Abubakar, BDJ George, OJ Ajanusi, SS Obeta, 1175- ...

Semi-automated detection of aberrant chromosomes in bivariate flow karyotypes

NARCIS (Netherlands)

Boschman, G. A.; Manders, E. M.; Rens, W.; Slater, R.; Aten, J. A.

1992-01-01

A method is described that is designed to compare, in a standardized procedure, bivariate flow karyotypes of Hoechst 33258 (HO)/Chromomycin A3 (CA) stained human chromosomes from cells with aberrations with a reference flow karyotype of normal chromosomes. In addition to uniform normalization of

Fraction against Human Cancer Cell Lines

African Journals Online (AJOL)

fraction of A. sieberi against seven cancer cell lines (Colo20, HCT116, DLD, MCF7, Jurkat, HepG2 and ... The morphology of the HepG2 cell nucleus was investigated by Hoechst 33342, ..... Gong F, Liang Y, Xie P, Chau F. Information theory.

The survival of cultured mouse cerebellar granule cells is not dependent on elevated potassium-ion concentration

DEFF Research Database (Denmark)

Mogensen, Helle Smidt; Hack, N; Balázs, R

1994-01-01

The effects of K(+)-induced membrane depolarization were studied on the survival and biochemical parameters in mouse and rat cerebellar granule cells grown in micro-well cultures. Cell numbers were determined by estimating DNA content using the Hoechst 33258 fluorochrome binding assay. DNA from d...

Engl 1102: Literature and Composition: Handwriting and Typography

Science.gov (United States)

Kashtan, Aaron

2015-01-01

Aaron Kashtan taught three sections of ENGL 1102, the second course in a mandatory first-year writing sequence with a heavy multimodal focus, during his first semester as a Marion L. Brittain Postdoctoral Fellow in the Department of Literature, Media and Communication at Georgia Tech. The specific subject matter for these sections was handwriting…

Both the Antarctic fur seal Arctocephalus gazella and the ...

African Journals Online (AJOL)

spamer

cause the pupping season continues into the second week of January (Kerley 1983b). On the open vegetated areas behind landing beaches, all fur seals were counted and classified. Pup numbers were derived from three sources: first, an estimate of the degree of polygyny. (2.4 pups per adult male) calculated for a Marion.

Lavakad, 23. lend / Eva Kübar

Index Scriptorium Estoniae

Kübar, Eva

2008-01-01

Eesti Muusika- ja Teatriakadeemia lavakunstikooli XXIII lennu lõpetajatest - näitlejad Marek Tammets, Ago Soots, Agnes Aaliste, Martin Mill, Andres Oja, Juss Haasma, Maarja Mitt, Meelis Põdersoo, Jekaterina Novosjolova, Elina Pähklimägi ja Kristo Viiding, dramaturgid Marion Jõepera, Mart Kase ja Maria Lee Liivak ning lavastajad Robert Annus ja Uku Uusberg

Author Details

African Journals Online (AJOL)

Ameh, GI. Vol 10 (2006) - Articles Crassulacean acid metabolism in some succulent plants. Abstract · Vol 10 (2006) - Articles Growth analysis studies of some accessions of African yam bean, Sphenostylis stenocarpa (Hoechst, ex. A. Rich.) Harms Abstract. ISSN: 1119-2283. AJOL African Journals Online. HOW TO USE ...

POPULATION AND BREEDING OF THE GENTOO PENGUIN ...

African Journals Online (AJOL)

The numbers of gentoo penguins Pygoscelis papua breeding at subantarctic Marion Island fell by 40% from 1994/95 to 2002/03, from 1 352 pairs to 806 pairs. Apart from a slight increase in 1998/99, there was a steady decrease in numbers breeding between 1995/96 and 2000/01, when the population stabilized. There is ...

Annual evapotranspiration of a forested wetland watershed, SC

Science.gov (United States)

Devendra M. Amatya; Carl Trettin

2007-01-01

In this study, hydro-meteorological data collected from 1 964 to 1 9 76 on an approximately 5, 000 ha predominantly forested coastal watershed (Turkey Creek) at the Francis Marion National Forest near Charleston, SC were analyzed to estimate annual evapotranspiration (E T) using four different empirical methods. The first one, reported by Zhang et a/. (2001), that...

Kauffman Teen Survey. An Annual Report on Teen Health Behaviors: Use of Alcohol, Tobacco, and Other Drugs among 8th-, 10th-, and 12th-Grade Students in Greater Kansas City, 1991-92 to 2000-01.

Science.gov (United States)

Ewing Marion Kauffman Foundation, Kansas City, MO.

The Ewing Marion Kauffman Foundation began surveying Kansas City area teens during the 1984-85 school year. The Kauffman Teen Survey now addresses two sets of issues for teens. Teen Health Behaviors, addressed in this report, have been a focus of the survey since its inception. The report focuses on teen use of alcohol, tobacco, and other drugs in…

A Clean Energy Roadmap: Forging the Path Ahead

Science.gov (United States)

Ewing Marion Kauffman Foundation, 2010

2010-01-01

In 2010, the Ewing Marion Kauffman Foundation co-convened three cross-sector summits to develop recommendations for growing energy innovation in the United States. The first summit was held in Washington, D.C., on May 7, 2010, in partnership with the White House. Gallup and the city of Omaha, Nebraska, hosted the second summit on June 16, 2010,…

Mis on elus olulisim / Aita Kivi

Index Scriptorium Estoniae

Kivi, Aita, 1954-

2002-01-01

Sisu : Mitch Albom. Teisipäevad Morrie'ga; Santa Montefiore. Oota mind ombupuu all; Ridley Pearson. Tont teab kus; Sebastian Faulks. Linnulaul; Kerttu Rakke. Iluasjake; Merike Hanni. Kooliraha; Viivi Luik. Ajaloo ilu; Hermann Hesse. Stepihunt; Eesti luulet läbi aegade / [koostanud Marika Vingissar; Philip K. Dick. Ubik; Marion Zimmer Bradley. Avaloni udud; Kuus eesti luuletajat / koostanud Anne Lange

Fire management ramifications of Hurricane Hugo

Science.gov (United States)

J. M. Saveland; D. D. Wade

1991-01-01

Hurricane Hugo passed over the Francis Marion National Forest on September 22, 1989, removing almost 75 percent of the overstory. The radically altered fuel bed presented new and formidable challenges to fire managers. Tractor-plows, the mainstay of fire suppression, were rendered ineffective. The specter of wind-driven escaped burns with no effective means of ground...

Study of the reactions 9Be(p, α)6Li, 9Be(p,d)8Be from 300 keV to 900 keV

International Nuclear Information System (INIS)

Bertrand, F.; Grenier, G.; Pornet, J.

1968-10-01

The experimental results concerning the two reactions 9 Be(p,α) 6 Li and 9 Be(p,d) 8 Be from 300 to 900 keV are presented. The angular distribution, excitation and total cross-section curves are expressed in absolute values after a normalization carried out using results given by Weber, Davis and Marion. (authors) [fr

Eestit kaitseb krediidipuuduse eest EL-i pühapaiste efekt / Villu Zirnask

Index Scriptorium Estoniae

Zirnask, Villu, 1966-

2007-01-01

Deutsche Bank Researchi analüütikud Marion Mühlberger ja Thorsten Nestmann peavad oma raportis Ida-Euroopas tõenäoliseks laenukasvu aeglustumist ehk pehmet maandumist. Kõige haavatavamaks peavad nad Kasahstani, Venemaa ja Läti pangasüsteemi. Reitinguagentuuri Fitch areneva Euroopa riigireitingute vanemdirektor Edward Parker põhjendab, miks Eestis ei peaks kartma suure finantskriisi tekkimist

Comparing vegetation cover in the Santee Experimental Forest, South Carolina (USA), before and after hurricane Hugo: 1989-2011

Science.gov (United States)

Giovanni R. Cosentino

2013-01-01

Hurricane Hugo struck the coast of South Carolina on September 21, 1989 as a category 4 hurricane on the Saffir-Simpson Scale. Landsat Thematic mapper was utilized to determine the extent of damage experienced at the Santee Experimental Forest (SEF) (a part of Francis Marion National Forest) in South Carolina. Normalized Difference Vegetation Index (NDVI) and the...

Mirounga leonina

African Journals Online (AJOL)

Marion Island and Antarctica (SANAE). JI. S. Afr. bioI. Soc. 3: 27-84. LAWS, R.M. 1953. The elephant seal (Mirounga leonina Linn.). I. Growth and age. Sci. Rep. Falkland Is. Dep. Surv. 8: 1-62. LAWS, R.M. 1956. The elephant seal (Mirounga leonina Linn.) II. General, social and reproductive behaviour. Sci. Rep. Falkland Is.

Status and perspectives of waste product utilisation in the T2C plant at Hoechst; Stand und Perspektiven in der EBS-Verwertung in der T2C-Anlage Hoechst

Energy Technology Data Exchange (ETDEWEB)

Lorbach, Dirk [Infraserv GmbH und Co. Hoechst KG, Frankfurt am Main (Germany). Geschaeftsfeld Energien

2012-11-01

The plant concept is based on combustion of a multitude of substitute fuels. According to the substitute fuel specifications for general entrepreneur EBARA, comminution of waste materials in a slow shredder should be sufficient, and experience so far has shown that this is indeed the case. The plant was commissioned in 2011, and the envisaged performance parameters were achieved after some initial difficulties, i.e. the envisaged volumes of waste could be combusted. The efficiency of heat generation was below expectations, however, as the integrated heat exchangers did not run at full capacity. Reconstruction of the plant is now planned with a view to optimizing the energy yield. It is expected that the energy efficiency goals will be reached in 2013. Modernisation will comprise retrofitting of a flue gas circulation system for better temperature control in the fluidized bed and for optimum energy yield. All other plant components - off-gas filters, water/steam cycle and turbine - work satisfactorily according to the planned parameters. (orig.) [German] Das Konzept der Anlage basiert auf der thermischen Verwertung einer Vielzahl von Ersatzbrennstoffen. Entsprechend der EBS-Spezifikation fuer den Generalunternehmer EBARA sollte fuer eine ausreichende Stueckgroesse eine Zerkleinerung der Abfaelle mit einem langsam laufenden Shredder ausreichen. In der bisherigen Inbetriebnahme hat sich gezeigt, dass dies grundsaetzlich von der Anlagentechnik, bis auf eine Einschraenkung, auch erreicht wird. Die Inbetriebnahme der Anlage wurde 2011 fuer den Dauerbetrieb abgeschlossen. Nach anfaenglichen Schwierigkeiten hat die Firma EBARA die vertraglichen Leistungsparameter im Bereich der Verbrennung erreicht. Die vorgesehenen EBS-Mengen koennen verbrannt werden. Bisher is allerdings die Waermeausbeute hinter den Erwartungen zurueckgeblieben, da die Einbauten in der Wirbelschicht, das sind integrierte Waermetauscher (HRC's), bisher ihre vollstaendige Leistung nicht erbracht haben. Ein entsprechender Umbau der Anlage wird derzeit geplant, um auch die Energieausbeute zu optimieren. Es wird erwartet, dass dadurch 2013 die erwartete Energieeffizienz erreicht wird. Im Rahmen des Umbauprogramms wird insbesondere die Nachruestung einer Rauchgasrezirkulation geplant, die eine verbesserte Temperaturfuehrung in der Wirbelschicht und eine optimierte Energieausbeute gewaehrleisten soll. Alle anderen Anlagenkomponenten, Abgasreinigung, Wasser-Dampf-Kreislauf und Turbine arbeiten entsprechend den geplanten Parametern zufriedenstellend. (orig.)

Evidence for particle transport between alveolar macrophages in vivo

Energy Technology Data Exchange (ETDEWEB)

Benson, J.M.; Nikula, K.J.; Guilmette, R.A.

1995-12-01

Recent studies at this Institute have focused on determining the role of alveolar macrophages (AMs) in the transport of particles within and form the lung. For those studies, AMs previously labeled using the nuclear stain Hoechst 33342 and polychromatic Fluoresbrite microspheres (1 {mu}m diameter, Polysciences, Inc., Warrington, PA) were instilled into lungs of recipient F344 rats. The fate of the donor particles and the doubly labeled AMs within recipient lungs was followed for 32 d. Within 2-4 d after instillation, the polychromatic microspheres were found in both donor and resident AMs, suggesting that particle transfer occurred between the donor and resident AMs. However, this may also have been an artifact resulting from phagocytosis of the microspheres form dead donor cells or from the fading or degradation of Hoechst 33342 within the donor cells leading to their misidentification as resident AMs. The results support the earlier findings that microspheres in donor AMs can be transferred to resident AMs within 2 d after instillation.

Association of Pinus banksiana Lamb. and Populus tremuloides Michx. seedling fine roots with Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina).

Science.gov (United States)

Potvin, Lynette R; Richter, Dana L; Jurgensen, Martin F; Dumroese, R Kasten

2012-11-01

Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina, Hydanaceae), commonly regarded as a wood decay fungus, was consistently isolated from bareroot nursery Pinus banksiana Lamb. seedlings. S. brinkmannii was found in ectomycorrhizae formed by Thelephora terrestris Ehrh., Laccaria laccata (Scop.) Cooke, and Suillus luteus (L.) Roussel. In pure culture combinations with sterile P. banksiana and Populus tremuloides Michx. seedlings, S. brinkmannii colonized root cortical cells while not killing seedlings. Colonization by S. brinkmannii appeared to be intracellular but typical endo- or ectomycorrhizae were not formed. The fungus did not decay roots, although it was shown to produce cellulase in enzyme tests. Results suggest a unique association between S. brinkmannii and seedling roots that is neither mycorrhizal nor detrimental; its exact function remains to be elucidated.

An iterative method for the canard explosion in general planar systems

DEFF Research Database (Denmark)

Brøns, Morten

2013-01-01

The canard explosion is the change of amplitude and period of a limit cycle born in a Hopf bifurcation in a very narrow parameter interval. The phenomenon is well understood in singular perturbation problems where a small parameter controls the slow/fast dynamics. However, canard explosions are a...... on the van der Pol equation, showing that the asymptotics of the method is correct, and on a templator model for a self-replicating system....... are also observed in systems where no such parameter can obviously be identied. Here we show how the iterative method of Roussel and Fraser, devised to construct regular slow manifolds, can be used to determine a canard point in a general planar system of nonlinear ODEs. We demonstrate the method...

Journal of Biosciences | Indian Academy of Sciences

Indian Academy of Sciences (India)

The expression patterns of CHOP and glucose-regulated protein (GRP) 78, a good marker of ER stress, were assessed by Western blotting, real-time PCR, Hoechst and immunohistochemistry in the hippocampus, cortex and striatum on a status epilepticus (SE) model. Double-fluorescent staining of CHOP and the terminal ...

A fluorescence in situ staining method for investigating spores and vegetative cells of Clostridia by confocal laser scanning microscopy and structured illuminated microscopy.

Science.gov (United States)

D'Incecco, P; Ong, L; Gras, S; Pellegrino, L

2018-04-18

Non-pathogenic spore-forming Clostridia are of increasing interest due to their application in biogas production and their capability to spoil different food products. The life cycle for Clostridium includes a spore stage that can assist in survival under environmentally stressful conditions, such as extremes of temperature or pH. Due to their size, spores can be investigated by a range of microscopic techniques, many of which involve sample pre-treatment. We have developed a quick, simple and non-destructive fluorescent staining procedure that allows a clear differentiation between spores and vegetative cells and effectively stains spores, allowing recovery and tracking in subsequent experiments. Hoechst 34580, Propidium iodide and wheat germ agglutinin WGA 488 were used in combination to stain four strains of Clostridia at different life cycle stages. Staining was conducted without drying the sample, preventing changes induced by dehydration and cells observed by confocal laser scanner microscopy or using a super-resolution microscope equipped with a 3D-structured illumination module. Dual staining with Hoechst/Propidium iodide differentiated spores from vegetative cells, provided information on the viability of cells and was successfully applied to follow spore production induced by heating. Super-resolution microscopy of spores probed by Hoechst 34580 also allowed chromatin to be visualised. Direct staining of a cheese specimen using Nile Red and Fast Green allowed in situ observation of spores within the cheese and their position within the cheese matrix. The proposed staining method has broad applicability and can potentially be applied to follow Clostridium spore behaviour in a range of different environments. Copyright © 2018 Elsevier Ltd. All rights reserved.

Design of a Bioactive Small Molecule that Targets the Myotonic Dystrophy Type 1 RNA Via an RNA Motif-Ligand Database & Chemical Similarity Searching

Science.gov (United States)

Parkesh, Raman; Childs-Disney, Jessica L.; Nakamori, Masayuki; Kumar, Amit; Wang, Eric; Wang, Thomas; Hoskins, Jason; Tran, Tuan; Housman, David; Thornton, Charles A.; Disney, Matthew D.

2012-01-01

Myotonic dystrophy type 1 (DM1) is a triplet repeating disorder caused by expanded CTG repeats in the 3′ untranslated region of the dystrophia myotonica protein kinase (DMPK) gene. The transcribed repeats fold into an RNA hairpin with multiple copies of a 5′CUG/3′GUC motif that binds the RNA splicing regulator muscleblind-like 1 protein (MBNL1). Sequestration of MBNL1 by expanded r(CUG) repeats causes splicing defects in a subset of pre-mRNAs including the insulin receptor, the muscle-specific chloride ion channel, Sarco(endo)plasmic reticulum Ca2+ ATPase 1 (Serca1/Atp2a1), and cardiac troponin T (cTNT). Based on these observations, the development of small molecule ligands that target specifically expanded DM1 repeats could serve as therapeutics. In the present study, computational screening was employed to improve the efficacy of pentamidine and Hoechst 33258 ligands that have been shown previously to target the DM1 triplet repeat. A series of inhibitors of the RNA-protein complex with low micromolar IC50’s, which are >20-fold more potent than the query compounds, were identified. Importantly, a bis-benzimidazole identified from the Hoechst query improves DM1-associated pre-mRNA splicing defects in cell and mouse models of DM1 (when dosed with 1 mM and 100 mg/kg, respectively). Since Hoechst 33258 was identified as a DM1 binder through analysis of an RNA motif-ligand database, these studies suggest that lead ligands targeting RNA with improved biological activity can be identified by using a synergistic approach that combines analysis of known RNA-ligand interactions with virtual screening. PMID:22300544

Defining Adaptive Leadership in the Context of Mission Command

Science.gov (United States)

2011-06-10

Schreiber. 2006. Complexity leadership theory : An interactive perspective on leading in complex adaptive systems. http://digitalcommons. unl.edu/cgi...Free Press. Schneider, Marguerite, and Mark Somers, Organizations as complex adaptive systems: Implications of complexity theory for leadership ...12 December 2010). Uhl-Bien, Mary, Russ Marion, and Bill McKelvey. Complexity leadership theory : Shifting leadership from the industrial age to the

William Healy, M.D., Father of the American Child Guidance Movement

Science.gov (United States)

1981-08-01

courts, was another important development, starting in 1899. Meyer, Sigmund Freud , and Healy all contributed to the next step, which coupled the...Dummer Papers lists 454 correspondents. The prominent psychiatrists include: Franz Alexander, Trigant Burrow, Havelock Ellis, Flanders Dunbar, Sigmund ... Freud , Roy Grinker, William Healy, Karen Homey, Marion E. Kenworthy, Lawson Lowrey, Julse Masserman, Karl Menninger, Adolf Meyer, Smith Ely Jelliffe

Bilimsel Toplantı Duyuruları

OpenAIRE

Adli Tıp Uzmanları Derneği ATUD

2005-01-01

The 2006 Techno Security Conference The Eighth Annual International Techno Security Conference Further information : June 4-7, 2006 Myrtle Beach Marriott Resort 8400 Costa Verde Drive Myrtle Beach, SC 29572 Medical Forensic Management of Adult Sexual Assault 16-18 June 2006, Auckland Further information : Marion Davis Library Building 43, Auckland Hospital, Park Road, Grafton Auckland The 6th Annual Digital Forensic Research Work...

Real-time and time-integrated PM2.5 and CO from prescribed burns in chipped and non-chipped plots: firefighter and community exposure and health implications

Science.gov (United States)

Luke P. Naeher; Gary L. Achtemeier; Jeff S. Glitzenstein; Donna R. Streng; David Macintosh

2006-01-01

In this study, smoke data were collected from two plots located on the Francis Marion National Forest in South Carolina during prescribed burns on 12 February 2003. One of the plots had been subjected to mechanical chipping, the other was not. This study is part of a larger investigation of fire behavior related to mechanical chipping, parts of which are presented...

Race as Seriality: A Response to David Benatar and Zimitri Erasmus

Science.gov (United States)

Gouws, A.

2010-01-01

In this article I draw on the Sarte's notion of "seriality" as theorized in his book "Dialectic of Reason" and as interpreted by Iris Marion Young. I argue that seriality can be used to escape the false essentialism and identity politics of race as a category for admissions to universities. A series is a social collective whose members are unified…

REMINDER FROM MEDIAL SERVICE

CERN Multimedia

Service médical

2000-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor. For information, call the Nurses - on Telephone: 73802. - by electronic mail: Infirmary.Service@cern.ch Marion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.ch

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Medical Service

2001-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor. For information, call the Nurses on Telephone: 73802. by electronic mail: Infirmary.Service@cern.ch Marion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.ch

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Medical Service

2000-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor. For information, call the Nurses on Telephone: 73802. by electronic mail: Infirmary.Service@cern.ch Marion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.ch

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Service Médical

2000-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor. For information, call the Nurses on telephone: 73802. by electronic mail to: Infirmary.Service@cern.chMarion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.ch Medical Service

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Service Médical

2000-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor.For information, call the Nurses- on Telephone73802- by electronic mailInfirmary.Service@cern.chMarion.Diedrich@cern.chJanet.Doody@cern.chMireille.Vosdey@cern.chMedical Service

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Service Médical

2000-01-01

iFor medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor. For information, call the Nurses on Telephone: 73802. by electronic mail: Infirmary.Service@cern.ch Marion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.ch

REMINDER FROM THE MEDICAL SERVICE

CERN Multimedia

Medical Service

2000-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor. For information, call the Nurses on Telephone: 73802 by electronic mail: Infirmary.Service@cern.ch Marion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.ch

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Medical Service

2000-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor.For information, call the Nurses on Telephone: 73802 or by electronic mail:Infirmary.Service@cern.chMarion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.chMedicalService

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Medical Service

2001-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor. For information, call the Nurses on telephone: 73802. by electronic mail: Infirmary.Service@cern.ch Marion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.ch

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Service médical

2000-01-01

For medical problems, we would like to remind all personnel working on the CERN sites,be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor.For information, call the Nurseson telephone: 73802.by electronic mail to:Infirmary.Service@cern.chMarion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.chMedicalService

REMINDER FROM MEDICAL SERVICE

CERN Multimedia

Medical Service

2001-01-01

For medical problems, we would like to remind all personnel working on the CERN sites, be they staff or from outside firms, that they are welcome at the Infirmary, building 57, ground floor. For information, call the Nurses on Telephone: 73802 by electronic mail: Infirmary.Service@cern.ch Marion.Diedrich@cern.ch Janet.Doody@cern.ch Mireille.Vosdey@cern.ch  

40 CFR 81.315 - Indiana.

Science.gov (United States)

2010-07-01

... County X LaPorte County X Marion County X Porter County: An area bound on the north by Lake Michigan, on the west by the Lake-Porter County line, on the south by I-80 and 90 and on the east by the LaPorte-Porter County line ..... X The remainder of Porter County...... X Vigo County X Warrick County 1 X Wayne...

T-dependent B-cell activation is signalled by an early increase in potassium influx

DEFF Research Database (Denmark)

Owens, T; Kaplan, J G

1982-01-01

both by incorporation of 3H-thymidine after 48 h of culture and by uptake of potassium (measured as 86Rb) after 15 h. Analysis of metaphase chromosomes stained with Hoechst dye 33258 from co-cultured T-enriched and nude lymphocytes (from female and male donors, respectively) mixed in the proportion 1...

[Population Council responsible for RU486 clinical trials in USA].

Science.gov (United States)

Aguillaume, C J

1993-04-01

As a result of the sudden political change that came with the Clinton Administration, RU-486's manufacturer, Roussel-Uclaf, and the Population Council agreed on April 20, 1992, on the manufacture and distribution of RU-486 in the US. In the US, there are less than 1.6 million induced abortions annually. From now on, US women will be able to have a choice between medical and surgical abortion. The Population Council and Roussel-Uclaf have had a contract since 1982. The Council is solely responsible for the phase 2 clinical trial of RU-486 in the US and other countries. It must present to the US Food and Drug Administration (FDA) an amendment allowing it to begin phase 3 clinical trials. The Council will also lead the US medical facilities in this study. It will identify partners for future production of RU-486 and its distribution in the US. It will also submit to FDA a New Drug Application (NDA). FDA will review the scientific literature on RU-486 and evaluate all data submitted by the Population Council. There are still obstacles to be surmounted. The Population Council must demonstrate good judgment when selecting the criteria for choosing a pharmaceutical firm before a Technical Committee which will be part of a group of players promoting women's health, scientific experts, and other interested parties. It must find the necessary funds to conduct the clinical trials and prepare the NDA. Phase 3 clinical trials in the US must have at least 2000 women. They will test RU-486's efficacy, safety, and acceptability among women choosing medical abortion over surgical abortion. Since the Council operates in almost all countries in the world, has innovated contraceptive research and development activities, and has been endorsed by the UN, product approval of RU-486 in the US will affect policy in all countries concerned about abortion.

DIGITAL FLOOD INSURANCE RATE MAP DATABASE, MARION COUNTY, FLORIDA

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — The Digital Flood Insurance Rate Map (DFIRM) Database depicts flood risk information and supporting data used to develop the risk data. The primary risk...

DIGITAL FLOOD INSURANCE RATE MAP DATABASE, MARION COUNTY, KY

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — The Digital Flood Insurance Rate Map (DFIRM) Database depicts flood risk information and supporting data used to develop the risk data. The primary risk...

DIGITAL FLOOD INSURANCE RATE MAP DATABASE, MARION COUNTY, USA

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — The Digital Flood Insurance Rate Map (DFIRM) Database depicts flood risk information and supporting data used to develop the risk data. The primary risk...

Flow Cytometry of the Side Population: Tips & Tricks

Directory of Open Access Journals (Sweden)

Irene Sales-Pardo

2006-01-01

Full Text Available Background: The Side Population (SP has become an important hallmark for the definition of the stem cell compartment, especially in the detection of these cells and in their physical isolation by fluorescence-activated cell sorting (FACS. SP cells are CD34neg and were discovered using ultraviolet excitation based on the efflux of Hoechst 33342 (Ho342. Although the method works as originally described, we believe that this method is difficult for most investigators. First, because the ability to discriminate SP cells is based on the differential retention of Ho342 during a functional assay; second, because of the difficulties in setting the right experimental and acquisition conditions; and third, because the analysis of the acquired data requires an extensive expertise on flow cytometry to accurately detect the SP events. Methods: First of all and mainly for the SP application, the laser beam paths were exhaustively checked to ensure the lowest coefficients of variation. Blood suspensions were prepared by erythrocyte lysis with ammonium chloride and hematopoietic cells were labeled with Ho342. Results: The Ho342 concentration and the staining procedure are critical for the optimal resolution of the SP cells. Although UV laser alignment is very important to resolve the dim tail that outlines the SP, the problem with Ho342 excitation is not the Hoechst Blue emission, but rather the Hoechst Red's (because of the weak emission. Conclusions: Each laboratory must establish its own expected ranges based on its instrument and results may vary slightly due to instrument differences such as the narrowness of the band pass filters, laser power, laser emission wavelength, nozzle type, differential of pressure, light collection system (cuvette versus jet-in-air and beam shaping optics.

Rainfall-runoff modeling of the Chapel Branch Creek Watershed using GIS-based rational and SCS-CN methods

Science.gov (United States)

Elizabeth N. Mihalik; Norm S. Levine; Devendra M. Amatya

2008-01-01

Chapel Branch Creek (CBC), located within the Town of Santee adjacent to Lake Marion in Orangeburg County, SC, is listed on the SC 2004 303(d) list of impaired waterbodies due to elevated levels of nitrogen (N), phosphorus (P), chlorophyll-a, and pH. In this study, using a GIS-based approach, two runoff modeling methods, the Rational and SCS-CN methods, have been...

Eesti teater tegi puhta töö / Reet Weidebaum

Index Scriptorium Estoniae

Weidebaum, Reet

2009-01-01

Heidelbergi kaasaegse dramaturgia festivalist "Stückemarkt 2009", kus sel aastal oli külaliseks Eesti oma algupäraste näidenditega - Andrus Kiviräha "Aabitsa kukk", Jim Ashilevi "Portselansuits", Urmas Lennuki "Boob teab". Kommentaare jagavad festivali kunstiline juht ning žürii liige Axel Preuss, tõlkija Irja Grönholm, žürii liikmed Nino Haratischwili, Marion Tiedke ja Andreas Jüttner

Ten years of real-time streamflow gaging of turkey creek - where we have been and where we are going

Science.gov (United States)

Paul Conrads; Devendra Amatya

2016-01-01

The Turkey Creek watershed is a third-order coastal plain stream system draining an area of approximately 5,240 hectares of the Francis Marion National Forest and located about 37 miles northwest of Charleston near Huger, South Carolina. The U.S. Department of Agriculture (USDA) Forest Service maintained a streamflow gaging station on Turkey Creek from 1964 to 1981....

Utmost requirements in an extreme situation; 'Hoechste Anforderungen in einer Extremsituation'

Energy Technology Data Exchange (ETDEWEB)

Aeberli, O. E.

2009-07-01

In this interview with the architect Ueli Lehmann, the planning and realisation of the highest restaurant in Europe - the Matterhorn Glacier Paradise near Zermatt in Switzerland - is discussed. Lehmann is the architect responsible for the building and discusses the special challenges placed on the restaurant in this high-alpine environment. Topics discussed also include the specific criteria valid for high-alpine building, the realisation of the building to meet the stringent 'Minergie-P' extremely low energy consumption standard, the building's indirect contributions to environmental protection, drinking water supplies, choice of materials, the building technical services and the photovoltaic installation, as well as snow and ice factors.

Uncertainty and Sensitivity Analysis for an Ibuprofen Synthesis Model Based on Hoechst Path

DEFF Research Database (Denmark)

da Conceicao Do Carmo Montes, Frederico; Gernaey, Krist V.; Sin, Gürkan

2017-01-01

into consideration the effects of temperature, acidity, and the choice of the catalyst. Parameter estimation and uncertainty analysis were conducted on the kinetic model parameters using experimental data available in the literature. Finally, one factor at a time sensitivity analysis in the form of deviations......The pharmaceutical industry faces several challenges and barriers when implementing new or improving current pharmaceutical processes, such as competition from generic drug manufacturers and stricter regulations from the U.S. Food and Drug Administration and the European Medicine agency. The demand...... for efficient and reliable models to simulate and design/improve pharmaceutical processes is therefore increasing. For the case of ibuprofen, a well-known anti-inflammatory drug, the existing models do not include its complete synthesis path, usually referring only to one out of aset of different reactions...

Quantitative gene expression profiling of CD45+ and CD45- skeletal muscle-derived side population cells

DEFF Research Database (Denmark)

Ditte Caroline Andersen, Ditte Caroline; Kristiansen, Gitte Qvist; Jensen, Line

2012-01-01

The skeletal muscle-derived side population (mSP) which highly excludes Hoechst 33342 is composed of CD45(+) and CD45(-) subpopulations; yet, rareness of mSP cells in general has complicated extensive quantitative analysis of gene expression profiles in primarily isolated mSP cells. Here, we desc...... a satellite cell subpopulation) remain in the mSPCD45(-) fraction, and we show that these cells express high levels of many of the known myogenic precursor/stem cell related markers, including Pax7 and Myf5.......The skeletal muscle-derived side population (mSP) which highly excludes Hoechst 33342 is composed of CD45(+) and CD45(-) subpopulations; yet, rareness of mSP cells in general has complicated extensive quantitative analysis of gene expression profiles in primarily isolated mSP cells. Here, we...... describe the isolation of adult mouse normal skeletal muscle residing SPCD45(+) and SPCD45(-) cells from a parent mononuclear muscle-derived cell (MDC) population. Relative quantitative real time PCR (RT-PCR) of 64 genes revealed that mSPCD45(-) compared with mSPCD45(+) was enriched for cells expressing...

Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts

Directory of Open Access Journals (Sweden)

ACA Mattos

2006-10-01

Full Text Available Praziquantel (PZQ is effective against all the evolutive phases of Schistosoma mansoni. Infected Biomphalaria glabrata snails have their cercarial shedding interrupted when exposed to PZQ. Using primary in vitro transformed sporocysts, labeled with the probe Hoechst 33258 (indicator of membrane integrity, and lectin of Glycine max (specific for carbohydrate of N-acetylgalactosamine membrane, we evaluated the presence of lysosomes at this evolutive phase of S. mansoni, as well as the influence of PZQ on these acidic organelles and on the tegument of the sporocyst. Although the sporocyst remained alive, it was observed that there was a marked contraction of its musculature, and there occurred a change in the parasite's structure. Also, the acidic vesicles found in the sporocysts showed a larger delimited area after contact of the parasites with PZQ. Damages to the tegument was also observed, as show a well-marked labeling either with Hoechst 33258 or with lectin of Glycine max after contact of sporocysts with the drug. These results could partially explain the interruption/reduction mechanism of cercarial shedding in snails exposed to PZQ.

Spatial and temporal distribution of onroad CO2 emissions at the Urban spatial scale

Science.gov (United States)

Song, Y.; Gurney, K. R.; Zhou, Y.; Mendoza, D. L.

2011-12-01

The Hestia Project is a multi-disciplinary effort to help better understand the spatial and temporal distribution of fossil fuel carbon dioxide (CO2) emission at urban scale. Onroad transportation is an essential source of CO2 emissions. This study examines two urban domains: Marion County (Indianapolis) and Los Angeles County and explores the methods and results associated with the spatial and temporal distribution of local urban onroad CO2 emissions. We utilize a bottom-up approach and spatially distribute county emissions based on the Annual Average Daily Traffic (AADT) counts provided by local Department of Transportation. The total amount of CO2 emissions is calculated by the National Mobile Inventory Model (NMIM) for Marion County and the EMission FACtors (EMFAC) model for Los Angeles County. The NMIM model provides CO2 emissions based on vehicle miles traveled (VMT) data at the county-level from the national county database (NCD). The EMFAC model provides CO2 emissions for California State based on vehicle activities, including VMT, vehicle population and fuel types. A GIS road atlas is retrieved from the US Census Bureau. Further spatial analysis and integration are performed by GIS software to distribute onroad CO2 emission according to the traffic volume. The temporal allocation of onroad CO2 emission is based on the hourly traffic data obtained from the Metropolitan Planning Orgnizations (MPO) for Marion County and Department of Transportation for Los Angeles County. The annual CO2 emissions are distributed according to each hourly fraction of traffic counts. Due to the fact that ATR stations are unevenly distributed in space, we create Thiessen polygons such that each road segment is linked to the nearest neighboring ATR station. The hourly profile for each individual station is then combined to create a "climatology" of CO2 emissions in time on each road segment. We find that for Marion County in the year 2002, urban interstate and arterial roads have

EFECTO DEL MEDIO DE CULTIVO EN EL DESARROLLO DE Suillus granulatus (L. Roussel y S. brevipes (Pk. Kuntze

Directory of Open Access Journals (Sweden)

Dulce Ma. Murrieta-Hernández

2014-01-01

Full Text Available La tasa de crecimiento micelial de los hongos ectomicorrícicos Suillus granulatus y S. brevipes, se evaluó en tres medios de cultivo (PDA, BAF y MNM con dos valores de pH (4.8 y 5.8, con el fin de seleccionar el mejor medio de cultivo. Las cepas se aislaron de esporomas colectados en el bosque de Pinus hartwegii del Parque Nacional Cofre de Perote, Veracruz, México. Se encontraron diferencias significativas (Tukey, P ≤ 0.05 en el área de crecimiento de ambas especies; los valores más altos se registraron en el medio PDA. Respecto a los valores de pH evaluados, no hubo diferencias significativas. Cada uno de los medios evaluados se puede utilizar para el cultivo de las cepas S. granulatus y S. brevipes dependiendo de los objetivos. El medio PDA fue el mejor sustrato para el crecimiento de las cepas. Se sugiere utilizar el medio BAF para la producción masiva de micelio para inóculo y el medio MNM se recomienda ya sea para el mantenimiento de las cepas o para pruebas de micorrización.

Blue Marsh Lake, Bernville Protective Works. Design Memorandum Number 13 Schuylkill River basin. Tulpehocken Creek, Pennsylvania

Science.gov (United States)

1975-03-01

Aetna Telephone and Telegraph Company Z60 E. Washington Avenue Meyerstown, PA 19602 Mr. James Vallosio, Supervisor Engineering Department Heidelberg...line: (1) Miscellaneous Book Volume 311, page 456, Berks County Records - Clarence W. Mengel and Lillian W. Mengel, his wife and Henry L. Kalbach and...Marion B. Kalbach , his wife - agreement ) and right of way to Bernville Borough Authority. (2) Mi ’ ellaneous Book Volume 311, Page 509, Berks County

Journal of Rehabilitation Research and Development. Volume 27 Number 3, Summer 1990

Science.gov (United States)

1990-01-01

spatial neglect. and use of micro - and concludes with Solution Implementation and Verifica- computers in therapy. The inevitable unevenness in qual- tion...Neurophysiol 30(1):41-45. 1990. 24. Voluntary Control of Submaximal Grip Strength. Contact: Martin J. Segura. MD. Servicio de Neurologia. Niebuhr BR. Marion R...September 10-L, 1990 Trieste. Italy MICRO SYSTEMS 1990, Berlin, Germany Contact: ICTP, Conference on Application of Physics in Contact: MESAGO. Messe

Deception in Covert Nuclear Weapons Development: A Framework to Identify, Analyze, and Mitigate Future Long-Term Deception Efforts

Science.gov (United States)

2016-03-01

launch of Sputnik in 1957. Private sector and criminal examples, such as the 2001 Enron scandal and the rapid expansion of the Colombian cocaine trade...presented to legislators during annual budget debates with significant overcharging for equipment and services. Once approved, the surplus money was then... environmental monitoring station on Marion Island in the Antarctic in 1986. Their purpose was to conduct a survey for building an airstrip to handle large

Annual Systems Engineering Conference (12th). Volume 1

Science.gov (United States)

2009-10-29

This Presentation will discuss : • Modularity: what it is, Pros and Cons , how it is used on LCS • Overview of extended systems – What’s the concern...is Export Approval # ACK219 (assigned IAW PRO -4527, PRO -3439). Marion L. Butterfield, Alaka Shivananda, and Dennis Schwarz (The Boeing Company...Other Tech Maturity Assessment Methods 7. SWOT (Strength, Weakness, Threat, Opportunity) Analysis 8. Conclusions & Recommendations GWU 3 Introduction The

Proceedings of the Military Operational Medicine Research Program Return-to-Duty Toolkit Working Group Symposium, 1-2 September 2015

Science.gov (United States)

2015-09-01

ton of data, so that’s why we didn’t pursue aim trace in the final study. DR. MARION: Interesting finding and consistent, I think, with functional MRI ... Tablet -Based Test Procedure   97 The results are printed on Standard Form 2216 and given to Service Members Test Procedures Hearing...Hearing Conservation   98 Android-based tablet allows experimenter to Scan QR code with audiogram, age, sex Test Procedures Hearing Conservation

Igavesti uuesti üllatav romaanivõistlus / Peeter Helme

Index Scriptorium Estoniae

Helme, Peeter, 1978-

2009-01-01

2008. a. romaanivõistlusel 1. auhinda välja ei antud. 2. auhinna võitis Milvi Martina Piiri romaan "Liblikad janus". 3. auhinna võitjaid oli kaks: Birk Rohelend romaaniga "Minu sõraline sõber" ja Marion Andra "Algolagnia". Ergutusauhinna võitnud romaanid: Liina Lüecke "Sügismüsteerium", Robert Kurvitza "Püha ja õudne lõhn", Robert Randma "Sigaret" ja Helju Petsi "Õnneõiteta sirelid"

Romaanivõistlus 2008 - naised ruulivad! / Peeter Helme

Index Scriptorium Estoniae

Helme, Peeter, 1978-

2009-01-01

2008. a. romaanivõistlusel 1. auhinda välja ei antud. 2. auhinna võitis Milvi Martina Piiri romaan "Liblikad janus". 3. auhinna võitjaid oli kaks: Birk Rohelend romaaniga "Minu sõraline sõber" ja Marion Andra "Algolagnia". Ergutusauhinna võitnud romaanid: Liina Lüecke "Sügismüsteerium", Robert Kurvitza "Püha ja õudne lõhn", Robert Randma "Sigaret" ja Helju Petsi "Õnneõiteta sirelid"

Novel competition 2008 - women's and genre literature / Peeter Helme

Index Scriptorium Estoniae

Helme, Peeter, 1978-

2009-01-01

2008. a. romaanivõistlusel 1. auhinda välja ei antud. 2. auhinna võitis Milvi Martina Piiri romaan "Liblikad janus". 3. auhinna võitjaid oli kaks: Birk Rohelend romaaniga "Minu sõraline sõber" ja Marion Andra "Algolagnia". Ergutusauhinna võitnud romaanid: Liina Lüecke "Sügismüsteerium", Robert Kurvitza "Püha ja õudne lõhn", Robert Randma "Sigaret" ja Helju Petsi "Õnneõiteta sirelid"

Evidence-based diabetes nutrition therapy recommendations are effective: the key is individualization

OpenAIRE

Franz, Marion J; Boucher, Jackie L; Evert, Alison B

2014-01-01

Marion J Franz,1 Jackie L Boucher,2 Alison B Evert3 1Nutrition Concepts by Franz, Inc., Minneapolis, MN, 2Minneapolis Heart Institute Foundation, Minneapolis, MN, 3Diabetes Care Center, University of Washington Medical Center, Seattle, WA, USA Abstract: Current nutrition therapy recommendations for the prevention and treatment of diabetes are based on a systematic review of evidence and answer important nutrition care questions. First, is diabetes nutrition therapy effective? Clinical trials...

University Curricula in the Marine Sciences and Related Fields. Academic Years 1969-70 and 1970-71.

Science.gov (United States)

1971-01-01

equipment to be temporarily installed and removed each cruise. The ACONA has a speed of nine knots and an en - durance of three weeks or 4500 miles...Professor of Biology Harville, John, Ph.D., Professor of Biology and Marine Science Kenk, Vida , M.S., Assistant Professor McMaster, Pauline, M.A...Associate Professor of Resource Policies and Utilization Marts , Marion E., Ph.D., Vice Provost; Director, Summer Quarter; Professor of Geography and

Near Surface Ocean Experimental Technology Workshop Proceedings Held at Naval Ocean Research and Development Activity, NSTL Station, Mississippi on 6-8 November 1979,

Science.gov (United States)

1980-02-01

have been made by Sippican Corporation of Marion, Massachusetts. An example of our use was a survey in ,June 1978 around the 2 km high Caryn Seamount ...Sound. McGraw-Hill, New York, 384 pp. 8. Wishner, K. F., 1979, The biomass and ecology of the deep-sea benthopelagic (near-bottom) zooplankton, Ph.D...COMPONENT PART NOTICE THIS PAPER IS A COMPONENT PART OF THE FOLLOWING COMPILATION REPORT: (TITLE): Near Sarface Ocean Experimental Technology Workshop

Nanovesicles released by Dictyostelium cells: a potential carrier for drug delivery.

Science.gov (United States)

Lavialle, Françoise; Deshayes, Sophie; Gonnet, Florence; Larquet, Eric; Kruglik, Sergei G; Boisset, Nicolas; Daniel, Régis; Alfsen, Annette; Tatischeff, Irène

2009-10-01

Nanovesicles released by Dictyostelium discoideum cells grown in the presence of the DNA-specific dye Hoechst 33342 have been previously shown to mediate the transfer of the dye into the nuclei of Hoechst-resistant cells. The present investigation extends this work by conducting experiments in the presence of hypericin, a fluorescent therapeutic photosensitizer assayed for antitumoral photodynamic therapy. Nanovesicles released by Dictyostelium cells exhibit an averaged diameter between 50 and 150 nm, as measured by transmission cryoelectron microscopy. A proteomic analysis reveals a predominance of actin and actin-related proteins. The detection of a lysosomal membrane protein (LIMP II) indicates that these vesicles are likely generated in the late endosomal compartment. The use of the hypericin-containing nanovesicles as nanodevices for in vitro drug delivery was investigated by fluorescence microscopy. The observed signal was almost exclusively located in the perinuclear area of two human cell lines, skin fibroblasts (HS68) and cervix carcinoma (HeLa) cells. Studies by confocal microscopy with specific markers of cell organelles, provided evidence that hypericin was accumulated in the Golgi apparatus. All these data shed a new light on in vitro drug delivery by using cell-released vesicles as carriers.

The use of fluorescent indoline dyes for side population analysis.

Science.gov (United States)

Kohara, Hiroshi; Watanabe, Kohei; Shintou, Taichi; Nomoto, Tsuyoshi; Okano, Mie; Shirai, Tomoaki; Miyazaki, Takeshi; Tabata, Yasuhiko

2013-01-01

Dye efflux assay evaluated by flow cytometry is useful for stem cell studies. The side population (SP) cells, characterized by the capacity to efflux Hoechst 33342 dye, have been shown to be enriched for hematopoietic stem cells (HSCs) in bone marrow. In addition, SP cells are isolated from various tissues and cell lines, and are also potential candidates for cancer stem cells. However, ultra violet (UV) light, which is not common for every flow cytometer, is required to excite Hoechst 33342. Here we showed that a fluorescent indoline dye ZMB793 can be excited by 488-nm laser, equipped in almost all the modern flow cytometers, and ZMB793-excluding cells showed SP phenotype. HSCs were exclusively enriched in the ZMB793-excluding cells, while ZMB793 was localized in cytosol of bone marrow lineage cells. The efflux of ZMB793 dye was mediated by ATP binding cassette (ABC) transporter Abcg2. Moreover, staining properties were affected by the side-chain structure of the dyes. These data indicate that the fluorescent dye ZMB793 could be used for the SP cell analysis. Copyright © 2012 Elsevier Ltd. All rights reserved.

Nuevo silo – Austria

Directory of Open Access Journals (Sweden)

Schmidt, W.

1971-11-01

Full Text Available The firm Waagner-Biro, collaborating with Farbwerke Hoechst, AG., of Frankfurt, and with assistance from the Austrian Government, has designed and produced a new type of silo, made of steel, intended for use in underdeveloped countries, in emergency areas, and in any zone where silos are needed which can be rapidly assembled and taken down, are light in weight and easy to transport. These silos can contain solids, liquids, and, with a special external treatment, also aggressive chemicals for agricultural purposes.La firma Waagner-Biro, en colaboración con la Farbwerke Hoechst AG de Frankfurt y bajo la eficaz promoción del Gobierno austríaco, fabrica este moderno silo de acero-trevira destinado a cubrir las necesidades de países en vía de desarrollo, de zonas catastróficas y, en general, de cualquier lugar donde se requiera gran rapidez de montaje y desmontaje, poco peso propio y facilidad subsiguiente de transporte y celeridad de entrega. Puede contener productos sólidos y líquidos y, aplicándole un recubrimiento especial, incluso materias químicas biológicamente agresivas.

Modification of the radiation sensitivity of human tumour cells by a bis-benzimidazole derivative

Energy Technology Data Exchange (ETDEWEB)

Smith, P J; Anderson, C O [Medical Research Council, Cambridge (UK)

1984-10-01

A comparison was made of the ability of either X-radiation or a DNA-specific ligand (the vital bis-benzimidazole dye; Hoechst 33342) to induce: cell killing, inhibition of de novo DNA synthesis, DNA strand breakage and the delay of cell division in human colon adenocarcinoma cells in vitro. Unlike radiation-induced cell killing, ligand-induced cytotoxicity appeared to be positively correlated with the extent of inhibition of de novo DNA synthesis-a feature consistent with the persistent binding of ligand molecules to nuclear DNA. Ligand-induced DNA strand-breaks disappeared slowly although ligand-treated cells retained apparently normal capacities to repair discrete radiogenic DNA strand-breaks. Pre-treatment of cells with Hoechst 33342 resulted in a dose-modifying enhancement of radiation resistance not associated with altered dosimetry for strand-break induction. However, radioresistance was accompanied by the protracted retention of cells in the G/sub 2/ phase of the cell cycle. We suggest that the results provide direct evidence that the retention of cells in G/sub 2/ phase is a sparing phenomenon and is triggered by the responses of chromatin domains to the presence of DNA damage.

DNA binding studies of tartrazine food additive.

Science.gov (United States)

Kashanian, Soheila; Zeidali, Sahar Heidary

2011-07-01

The interaction of native calf thymus DNA with tartrazine in 10 mM Tris-HCl aqueous solution at neutral pH 7.4 was investigated. Tartrazine is a nitrous derivative and may cause allergic reactions, with a potential of toxicological risk. Also, tartrazine induces oxidative stress and DNA damage. Its DNA binding properties were studied by UV-vis and circular dichroism spectra, competitive binding with Hoechst 33258, and viscosity measurements. Tartrazine molecules bind to DNA via groove mode as illustrated by hyperchromism in the UV absorption band of tartrazine, decrease in Hoechst-DNA solution fluorescence, unchanged viscosity of DNA, and conformational changes such as conversion from B-like to C-like in the circular dichroism spectra of DNA. The binding constants (K(b)) of DNA with tartrazine were calculated at different temperatures. Enthalpy and entropy changes were calculated to be +37 and +213 kJ mol(-1), respectively, according to the Van't Hoff equation, which indicated that the reaction is predominantly entropically driven. Also, tartrazine does not cleave plasmid DNA. Tartrazine interacts with calf thymus DNA via a groove interaction mode with an intrinsic binding constant of 3.75 × 10(4) M(-1).

Polymer Based Molecular Composites. Volume 171. Materials Research Society Symposium Proceedings Held in Boston, Massachusetts on 27-30 November 1989

Science.gov (United States)

1990-09-01

reactivity features promoted by the polymeric sulfonic acid moiety in contrast to HCI. Further work is being undertaken utilizing para toluene 21 sulfonic...styrene and isoprene block and homo- polymers were synthesized by anionic polymerization. Cyclohexane (Burdick and Jackson HPLC Grade) was degassed and...terephthalic acid and 21 mole-% 4 aminophenol , was purchased from Hoechst Celanese. Blends containing 70 weight-% PPE and 30 weight-% PS were kindly

Proceedings of the International Wire and Cable Symposium (29th) Held at Cherry Hill, New Jersey on November 18, 19 and 20, 1980.

Science.gov (United States)

1980-11-01

Comunicaciones , S.A. Elizabeth, NJ Zaragoza, SPAIN Ilmercable Corporation Cabot Corporation El Dorado, Arkansas Boston, MA American Hoechst Corporation...FILLING RELATIONSHIP TO TRANSMISSION, V. Abadia, J. Z. COMPOUND, D. M. Mitchell, R. Sabia, Bell Lab- Avalos, Cables de Comunicaciones , S. A., Zaragoza...RESISTANCE PROBLEMS AND ITS RELATIONSHIP TO TRANSMISSION V. Abadia and J.Z. Avalos CABLES DE COMUNICACIONES , S,A, ABSTRACT TABLE I A good number of

Viral Oncolytic Therapeutics for Neoplastic Meningitis

Science.gov (United States)

2014-09-01

ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER Massachusetts General Hospital RICHARD BRINGHURST, M.D. 55 FRUIT ST BOSTON...Martuza. The cell lines were tested for mycoplasma, Hoechst DNA staining, PCR, and culture testing for contaminant bacteria, yeast, and fungi ...complication of breast cancer affects 5-8% of patients when circulating cancer cells seed in the meninges. Their subsequent growth causes severe

Eugeni Granell. La pintura y el lenguaje o cómo transgredir a Marcel Duchamp y a Raymond Roussel

Directory of Open Access Journals (Sweden)

X. Antón Castro

2013-11-01

Full Text Available La atmósfera dadá y surreal, que se esconde en el mundo pictórico de Granell, alcanza a su conside- ración del ser humano como cuerpo, amor, sexo, erotismo, mujer, mujer-máquina, mujer-fantástica, bajo unos soportes conceptuales que fían su discurso al humor, al azar, a la ironía, a la metaironía y a un mirar voyeurista, donde el espectador debe ser siempre un cómplice, todo lo cual se refuerza con la utilización críptica del lenguaje a partir de sus títulos barrocos. The Dada and surreal atmosphere that hides in Granell’s pictorial world refers to his consideration of the human being as body, love, sex, eroticism, woman, machine-woman, fantastic-woman. His conceptual bases build the discourse on humor, chance, irony, meta-irony and on a voyeuristic look, where the spectator must always be an accomplice. All this is reinforced by a cryptic use of the language, evident in his baroque titles.

2014 Marion County, GA DMC 4-Band 8 Bit Imagery

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This data set consists of one-foot pixel resolution, natural color orthoimages covering the urban area footprint. An orthoimage is remotely sensed image data in...

Töö röövib lastelt vanemad / Marion Pajumets

Index Scriptorium Estoniae

Pajumets, Marion

2012-01-01

Triin Roosalu Tallinna Ülikoolis kaitstud doktoriväitekirjast "Taking care of children and work in Estonian society : running out of post-socialist time?", mis käsitleb Eesti töö ja pereelu ühitamise mudelit

Doktoriväitekiri töö ülimuslikkusest / Marion Pajumets

Index Scriptorium Estoniae

Pajumets, Marion

2012-01-01

Triin Roosalu Tallinna Ülikoolis kaitstud doktoriväitekirjast "Taking care of children and work in Estonian society : running out of post-socialist time?", mis käsitleb Eesti töö ja pereelu ühitamise mudelit

Penitenciaría federal de Marion. Illinois (U. S. A.

Directory of Open Access Journals (Sweden)

Hellmuth, Obata y Kassabaum, Arquitectos

1966-09-01

Full Text Available This federal prison is designed to house 600 prisioners, and has been planned so that it can be easily enlarged, to accommodate 1,000 inmates. The distribution is radial, and entry is via an administrative building, which lies outside the double metallic safety enclosure. A main corridor leads to the central nucleus, from which three other corridors branch off. The one on the right connects with five units, containing the cells, a general dormitory, the library, public hall, gymnasium and a school. The central corridor leads to further cell units, the admission office, hospital and secondary services, whilst the left hand corridor provides access to the chapel, the prisoners dining mess, kitchens and washing rooms. The building is of high quality and has a noble aspect, following modern trend in this type of constructions.Esta penitenciaría Federal ha sido construida para albergar 600 reclusos, pero con la posibilidad de poder ampliar fácilmente su capacidad hasta 1.000. Al conjunto, que presenta una distribución general radial, se accede a través del edificio de la Administración situado a la entrada, fuera de la doble cerca metálica de seguridad. A través del corredor principal, se llega al núcleo central, del que parten otros tres corredores; el de la derecha consta de cinco unidades constructivas, que contienen las celdas de los reclusos y una unidad dormitorio, la biblioteca, el auditorio-gimnasio y la escuela. Al corredor del fondo dan otras unidades con celdas, la unidad de admisión, hospital, etc.; y al corredor de la izquierda: la capilla, el comedor de reclusos, las zonas de servicio, cocinas, lavandería, etc. El edificio es noble y de calidad, de acuerdo con las modernas directrices en este tipo de construcciones.

The Sutton (13MA266) Site and the Townsites of Percy (13MA347) and Dunreath (13MA449): Data Recovery at Three Historic Sites, Lake Red Rock, Iowa

Science.gov (United States)

1989-07-01

farm also is done by truck chiefly by common carriers although much milk and cream ;s hauled by contract haulers. The development of Marion County...chimney, and a whole medicine bottle. The latter had an improved tooled cork closure and was embossed with the following: I Chamberlain’s/ Colic Cholera...merchants did become actively involved in the truck shipment of eggs, butter, and cream to Des Moines. In later years all the store supplies were brought

New Planetariums For Old

Science.gov (United States)

Peterson, David

2005-11-01

The audio and visual capabilities of the planetarium at Francis Marion University were upgraded in Fall 2004 to incorporate three Barco CRT projectors and surround sound. Controlled by the Astro-FX media manager system developed by Bowen Technovation, the projectors focus on the 33 foot dome installed in 1978 for the Spitz 512 Star projector. The significant additional capabilities of the new combined systems will be presented together with a review of the planetarium renovation procedure.

Bagatellid : maailm / Nele-Eva Steinfeld, Ivo Heinloo

Index Scriptorium Estoniae

Steinfeld, Nele-Eva

2010-01-01

Lühisõnumaid maailmast: Vene pianist Julianna Avdejeva saavutas XVI rahvusvahelisel Chopini konkursil Varssavis võidu. Suri poola helilooja Henryk Mikolaj Gorecki. Daniel Barenboimi 60 lava-aastat. Suri dirigent ja vioolamängija Rudolf Baršai. Kitarrilegend Sonny Sharrock sai oma kodulinnas enda nimelise tänava. 23. Thelonious Monki nimelise jazzkonkursi võitis Cecile McLorin Salvant. Youn Sun Nah Prantsuse müügiedetabeli tipus. Ansamblil Jamiroquai ilmus uus plaat. Suri saksofonist Marion Brown

Effects of the Huguenot Diaspora on the American Revolution

Science.gov (United States)

2016-06-10

Garland McGee, Mr. Harry DuBose, Mr. Edward Thomas, Ms. Lindie Newman , Mr. Mike Cantine, Ms. Carolyn Goff, Ms. Michelle Miller, and Dr. Marion Paris...to serve this young gentleman, and act a Part towards him which I am sure would be exceedingly agreeable to my Parents , who always expressed the...117 By far the most entertaining connection is that of the eccentric Peter Billière, veteran of the British Army, and self- styled “Christian Patriot

YOGA CLUB

CERN Document Server

CLUB DE YOGA

2010-01-01

DÉBUT DES COURS   mercredi 1er septembre 2010   (Yoga, Sophrologie, Tai Chi, Zen) Liste des cours pour le semestre allant du 1 er septembre 2010 au 31 janvier 2011. New: one course will be held in English Viniyoga (progressive and adaptable yoga, emphasis on breathing) Courses held in English Teacher: Marta Csatari 1 course per week Tuesday 12h15 – 13h30 Viniyoga (yoga progressif et adapté, appui sur la respiration) Professeur : Magali Roussel 2 cours hebdomadaires Lundi       11h15 – 12h30                12h30 – 13h45 (débutants) Hatha Yoga et yoga chinois (tonification musculaire) Professeur : Marie-Claude Pihet 2 cours hebdomadaires Mardi      17h30 – 18h30 &nb...

Montelukast induced acute hepatocellular liver injury

Directory of Open Access Journals (Sweden)

Harugeri A

2009-01-01

Full Text Available A 46-year-old male with uncontrolled asthma on inhaled albuterol and formoterol with budesonide was commenced on montelukast. He developed abdominal pain and jaundice 48 days after initiating montelukast therapy. His liver tests showed an increase in serum total bilirubin, conjugated bilirubin, aspartate aminotranferase, alanine aminotranferase, and alkaline phosphatase. The patient was evaluated for possible non-drug related liver injury. Montelukast was discontinued suspecting montelukast induced hepatocellular liver injury. Liver tests began to improve and returned to normal 55 days after drug cessation. Causality of this adverse drug reaction by the Council for International Organizations of Medical Sciences or Roussel Uclaf Causality Assessment Method (CIOMS or RUCAM and Naranjo′s algorithm was ′probable′. Liver tests should be monitored in patients receiving montelukast and any early signs of liver injury should be investigated with a high index of suspicion for drug induced liver injury.

PS overcomes two serious magnet failures

CERN Multimedia

Maximilien Brice

2003-01-01

Two magnets (no.'s 6 and 19)and a bus bar connection in the PS were found to be faulty during high-voltage tests at the end of the accelerator shutdown. A five-week repair schedule was quickly devised. A team of mechanics, technicians and engineers worked at full speed to replace the faulty magnets, succeeding in limiting the delay of the accelerators' spring start-up to two weeks. Pictured here are members of the PS team with the replacement no. 6 magnet. From left to right: In the back row, Frédéric Roussel (Transport DBS), Yves Bernard (Transport DBS), Luc Moreno (Cegelec), Thierry Battimanza (Transport DBS), Raymond Brown (AB/ABP), Thomas Zickler (AT/MEL); at the front, Steven Southern (AT/VAC), Thierry Gaidon (Brun & Sorensen), Philippe Vidales (Cegelec), Daniel Aubert (Cegelec), Jerome Cachet (Transport DBS), Jose Manual Gomes de Faria (AT/MEL), Eric Page (AT/VAC).

Pärnus on uus nüüdiskunsti galerii!

Index Scriptorium Estoniae

2004-01-01

17. VI Pärnus Lõuna t. 20 avatud eksperimentaalne Rael Artel Gallery keskendub esimesel hooajal eelkõige foto ja sound art'i eksponeerimisele. Kuraatorid: Anu Allas, Andreas W, EKA kunstiteaduse üliõpilased Marion Tupits, Aleksander Tsapov, Urmas Oja. Galeristi Rael Arteli kureeritud avanäitusel "Jagatud kehad" on väljas Eveli Variku, Tanja Muravskaja, Imre Malva, Andreas W ja Haide Rannakivi tööd. Galerii audiogalerii avatakse Projekt Unisoni (Hello Upan, Raul Keller, Heikki Tikas) mürateosega "DOHC"

O funcionamento de mecanismos coesivos na argumentação

Directory of Open Access Journals (Sweden)

Barbisan, Leci Borges

2001-01-01

Full Text Available Estuda-se neste trabalho o funcionamento de dois mecanismos coesivos no texto argumentativo: a elipse e a anáfora conceitual. Parte-se da hipótese de que ambos desempenham papel no processo argumentativo. Para as análises, utilizam-se conceitos da teoria da argumentação na língua, propostos por Jean-Claude Anscombre e Oswald Ducrot, em sua primeira versão, e Marion Carel e Oswald Ducrot, em seu segundo momento

Nutritional compensation to exercise- vs. diet-induced acute energy deficit in adolescents with obesity

OpenAIRE

Thivel , David; Doucet , Eric; Julian , Valérie; Cardenoux , Charlotte; Boirie , Yves; Duclos , Martine

2017-01-01

This article belongs to a special issueConference: 24th Annual Meeting of the Society-for-the-Study-of-Ingestive-Behavior (SSIB)Location: Porto, PORTUGALDate: JUL 12-16, 2016Sponsor(s):Soc Study Ingest BehavThe authors want to thank the adolescents who took part in the study as well as Miss Nais Petiot and Miss Audrey Marion for their help; BACKGROUND: To compare the energy and macronutrient intake responses to equivalent energy deficits induced by diet (food restriction) and exercise in adol...

Measurements and simulation of the interaction of turbulence and premixed flames; Messungen und Simulationen zur Wechselwirkung zwischen Turbulenz und vorgemischten Flammen

Energy Technology Data Exchange (ETDEWEB)

Durst, B

2000-11-01

The interaction between turbulence and hydrogen-flames was investigated in an explosion tube. The flow velocity around single flow obstacles was measured with a laser-Doppler system and compared to the flame velocity which was recorded using photodiodes. The highest turbulence intensity (up to 10 m/s) and correspondingly the highest flame acceleration was measured in the shear layer downstream of the obstacle with the highest blockage ratio. A closure model based on probability density functions (PDF) was developed for the time averaged chemical reaction rate for the purpose of simulating turbulent combustion processes. Comparisons of the results gained from simulations using the PDF combustion modell showed good agreement with the measurements performed. [German] Die Wechselwirkung zwischen Turbulenz und Wasserstoff-Flammen wurde in einem Explosionsrohr untersucht. Die Stroemungsgeschwindigkeit wurde mit einem Laser-Doppler System an Einzelhindernissen gemessen und mit der Flammengeschwindigkeit, die mittels Photodioden erfasst wurde, verglichen. In der Scherschicht hinter dem Hindernis mit der hoechsten Blockierrate wurde die hoechste Turbulenzintensitaet (bis 10 m/s) und damit die hoechste Flammenbeschleunigung gemessen. Fuer numerische Simulationen der turbulenten Verbrennung wurde ein Schliessungsansatz fuer die zeitgemittelte chemische Reaktionsrate entwickelt, der auf Wahrscheinlichkeitsdichtefunktionen (englisch: PDF) basiert. Vergleichsrechnungen mit dem PDF-Verbrennungsmodell zeigten gute Uebereinstimmung mit den durchgefuehrten Messungen.

Terpenoids Isolated From the Shoot of Plectranthus hadiensis Induces Apoptosis in Human Colon Cancer Cells Via the Mitochondria-Dependent Pathway.

Science.gov (United States)

Menon, Darsan B; Gopalakrishnan, V K

2015-01-01

The plant Plectranthus hadiensis is a rich source of many bioactive phytochemicals, especially terpenoids. The terpenoid fraction was isolated and phytochemical characterization was done using GC-MS. The aim of the present study was to find out the antiproliferative activity and the mechanism of cell death induction by the terpenoid fraction on human colon cancer cells (HCT-15). MTT assay was performed with different concentrations of the fraction (10, 20, and 50 µg/mL) to obtain IC50 value for 24 h to induce cell death. The induction of apoptosis were studied by Hoechst staining, acridine orange/ethidium bromide staining, Comet assay, DNA fragmentation, and caspase-3 activity assays. The mechanism of apoptosis induction was studied by expression analysis of antiapoptotic Bcl-2 and proapoptotic Bax using RT-PCR and also by Western blot analysis of proteins involved in the apoptotic pathway. The terpenoid fraction induced significant morphological changes and DNA fragmentation in the cells. Positive Hoechst staining and acridine orange/ethidium bromide staining indicated apoptosis induction by the fraction. DNA fragmentation, which is a characteristic feature of apoptosis, was also observed. Upregulation of caspase-3 activity and proapoptotic Bax, and the downregulation of antiapoptotic Bcl-2 and COX-2 confirmed that the apoptosis induction was via the mitochondria-dependent pathway.

Synthetic Beta-Lactam Antibiotics as a Selective Breast Cancer Cell Apoptosis Inducer: Significance in Breast Cancer Prevention and Treatment

Science.gov (United States)

2008-03-01

Hoechst 33258, cremophore, dimethylsulfoxide ( DMSO ) and trypan blue were purchased from Sigma-Aldrich (St. Louis, MO). Apoptag peroxidase in situ...treated with solvent ( DMSO ), 50 or 100 μM of each indicated compound for 24 h, followed by trypan blue exclusion assay. (C) HL-60 leukemic cells were...treated with solvent ( DMSO ), 50 or 100 μM of each indicated analog for 24 h, followed by trypan blue exclusion assay. The numbers given are

Antilogic

OpenAIRE

Benoît Castelnérac; Mathieu Marion

2013-01-01

This paper is an interim report of joint work begun in (Castelnérac & Marion 2009) on dialectic from Parmenides to Aristotle. In the first part we present rules for dialectical games, understood as a specific form of antilogikê developed by philosophers, and explain some of the key concepts of these dialectical games in terms of ideas from game semantics. In the games we describe, for a thesis A asserted by the answerer, a questioner must elicit the answerer’s assent to further assertions B1,...

Synoptic surveys of major reservoirs in South Carolina, 1988--1989

International Nuclear Information System (INIS)

Bowers, J.A.

1992-03-01

Comprehensive synoptic surveys of ten South Carolina airs (L Lake, Savannah River Site (SRS), Par Pond, SRS, Pond B, SRS, Lake Moultrie, Lake Marion, Lake Murray, Lake Monticello, Lake Robinson, Lake Richard B. Russell, and Lake Greenwood) were performed to characterize and compare these basins with regard to water quality, trophic status, and community structure during September 1988 and September 1989. All of the reservoirs were mesoeutrophic to eutrophic having significantly greater productivity rates than oligotrophic ecosystems. This report presents and discusses the results of these surveys

A Challenge for Diagnosing Acute Liver Injury with Concomitant/Sequential Exposure to Multiple Drugs: Can Causality Assessment Scales Be Utilized to Identify the Offending Drug?

Directory of Open Access Journals (Sweden)

Roxanne Lim

2014-01-01

Full Text Available Drug-induced hepatotoxicity most commonly manifests as an acute hepatitis syndrome and remains the leading cause of drug-induced death/mortality and the primary reason for withdrawal of drugs from the pharmaceutical market. We report a case of acute liver injury in a 12-year-old Hispanic boy, who received a series of five antibiotics (amoxicillin, ceftriaxone, vancomycin, ampicillin/sulbactam, and clindamycin for cervical lymphadenitis/retropharyngeal cellulitis. Histopathology of the liver biopsy specimen revealed acute cholestatic hepatitis. All known causes of acute liver injury were appropriately excluded and (only drug-induced liver injury was left as a cause of his cholestasis. Liver-specific causality assessment scales such as Council for the International Organization of Medical Sciences/Roussel Uclaf Causality Assessment Method scoring system (CIOMS/RUCAM, Maria and Victorino scale, and Digestive Disease Week-Japan were applied to seek the most likely offending drug. Although clindamycin is the most likely cause by clinical diagnosis, none of causality assessment scales aid in the diagnosis.

Cd-tolerant Suillus luteus: a fungal insurance for pines exposed to Cd.

Science.gov (United States)

Krznaric, Erik; Verbruggen, Nathalie; Wevers, Jan H L; Carleer, Robert; Vangronsveld, Jaco; Colpaert, Jan V

2009-05-01

Soil metal pollution can trigger evolutionary adaptation in soil-borne organisms. An in vitro screening test showed cadmium adaptation in populations of Suillus luteus (L.: Fr.) Roussel, an ectomycorrhizal fungus of pine trees. Cadmium stress was subsequently investigated in Scots pine (Pinus sylvestris L.) seedlings inoculated with a Cd-tolerant S. luteus, isolated from a heavy metal contaminated site, and compared to plants inoculated with a Cd-sensitive isolate from a non-polluted area. A dose-response experiment with mycorrhizal pines showed better plant protection by a Cd-adapted fungus: more fungal biomass and a higher nutrient uptake at high Cd exposure. In addition, less Cd was transferred to aboveground plant parts. Because of the key role of the ectomycorrhizal symbiosis for tree fitness, the evolution of Cd tolerance in an ectomycorrhizal partner such as S. luteus can be of major importance for the establishment of pine forests on Cd-contaminated soils.

Albendazole Induced Recurrent Acute Toxic Hepatitis: A Case Report.

Science.gov (United States)

Bilgic, Yilmaz; Yilmaz, Cengiz; Cagin, Yasir Furkan; Atayan, Yahya; Karadag, Nese; Harputluoglu, Murat Muhsin Muhip

2017-01-01

Drug induced acute toxic hepatitis can be idiosyncratic. Albendazole, a widely used broad spectrum antiparasitic drug is generally accepted as a safe drug. It may cause asymptomatic transient liver enzyme abnormalities but acute toxic hepatitis is very rare. Case Report : Herein, we present the case of 47 year old woman with recurrent acute toxic hepatitis after a single intake of albendazole in 2010 and 2014. The patient was presented with symptoms and findings of anorexia, vomiting and jaundice. For diagnosis, other acute hepatitis etiologies were excluded. Roussel Uclaf Causality Assessment Method (RUCAM) score was calculated and found to be 10, which meant highly probable drug hepatotoxicity. Within 2 months, all pathological findings came to normal. There are a few reported cases of albendazole induced toxic hepatitis, but at adults, there is no known recurrent acute toxic hepatitis due to albendazole at this certainty according to RUCAM score. Physicians should be aware of this rare and potentially fatal adverse effect of albendazole. © Acta Gastro-Enterologica Belgica.

DCS Terrain Submission for City of Marion - 2m LiDAR

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describes the digital topographic data that was used to create...

Aerial Photography and Imagery, Ortho-Corrected - 2010 Digital Orthophotos - Marion County

Data.gov (United States)

NSGIC Education | GIS Inventory — This data set is one component of a digital orthophoto imagery (DOI) coverage over the Southwest Florida Water Management District (SWFWMD) North District Area, for...

Geologic map of the Maumee quadrangle, Searcy and Marion Counties, Arkansas

Science.gov (United States)

Turner, Kenzie J.; Hudson, Mark R.

2010-01-01

This map summarizes the geology of the Maumee 7.5-minute quadrangle in northern Arkansas. The map area is in the Ozark plateaus region on the southern flank of the Ozark dome. The Springfield Plateau, composed of Mississippian cherty limestone, overlies the Salem Plateau, composed of Ordovician carbonate and clastic rocks, with areas of Silurian rocks in between. Erosion related to the Buffalo River and its tributaries, Tomahawk, Water, and Dry Creeks, has exposed a 1,200-ft-thick section of Mississippian, Silurian, and Ordovician rocks mildly deformed by faults and folds. An approximately 130-mile-long corridor along the Buffalo River forms the Buffalo National River that is administered by the National Park Service. McKnight (1935) mapped the geology of the Maumee quadrangle as part of a larger 1:125,000-scale map focused on understanding the lead and zinc deposits common in the area. Detailed new mapping for this study was compiled using a Geographic Information System (GIS) at 1:24,000 scale. Site location and elevation were obtained by using a Global Positioning Satellite (GPS) receiver in conjunction with a U.S. Geological Survey 7.5-minute topographic map and barometric altimeter. U.S. Geological Survey 10-m digital elevation model data were used to derive a hill-shade-relief map used along with digital orthophotographs to map ledge-forming units between field sites. Bedding attitudes were measured in drainage bottoms and on well-exposed ledges. Bedding measured at less than 2 degree dip is indicated as horizontal. Structure contours constructed for the base of the Boone Formation are constrained by field-determined elevations on both upper and lower formation contacts.

Aerial Photography and Imagery, Ortho-Corrected - 2009 Digital Orthophotos - Marion County

Data.gov (United States)

NSGIC Education | GIS Inventory — This data set is one component of a digital orthophoto imagery (DOI) coverage over the Southwest Florida Water Management District (SWFWMD) North District Area, for...

2003 Southwest Florida Water Management District (SWFWMD) LiDAR: Marion County

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Merrick and Company produced one foot contours, spot elevations, and obscured areas for the Southwest Florida Water Management District.

Additional file 4: of MHC class II expression and potential antigen-presenting cells in the retina during experimental autoimmune uveitis

OpenAIRE

Lipski, Deborah; Dewispelaere, RÊmi; Foucart, Vincent; Caspers, Laure; Defrance, Matthieu; Bruyns, Catherine; Willermain, François

2017-01-01

Figure S4. MHC class II expression in the retina during classical EAU. Three weeks after immunization, eye cryosections were prepared and stained for MHC class II (green) and IBA1 (red) or endoglin (magenta) detection. Cell nuclei were stained with Hoechst (blue). Each picture was chosen as representative of an experiment conducted on six or more animals. A. MHC class II and IBA1 expression. B. MHC class II and endoglin expression. (PPTX 7276 kb)

Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases

OpenAIRE

Cha, Jeong-Dan; Kim, Ji-Young

2012-01-01

Cryptomeria japonica D. Don (C. japonica) has been used in traditional medicines from Asia for a variety of indications, including liver ailments, and an antitussive, and for its antiulcer activities. We examined the cell viability and apoptosis of KB cells treated with C. japonica essential oil at several concentrations for 12 h by MTT assay, Hoechst-33258 dye staining, DNA fragmentation, flow cytometry (cell cycle), and Western blotting for mitochondria stress, activation of caspases, and p...

Erfolgsfaktoren unternehmerischer Krisen-PR

OpenAIRE

Scharr, Florian

2006-01-01

Ob "Peanuts", "Elchtest" oder "Hoechst-Störfall" - bei diesen bekannten Öffentlichkeitskrisen der letzten Jahre rief die mangelhafte Krisen-PR der betroffenen Unternehmen bei Medien und Bevölkerung mehr Empörung hervor als die originären Auslöser und potenzierte so den entstandenen Vertrauensverlust. Auch die sich gerade erst entwickelnde PR-Forschung bietet noch keine konkrete Hilfestellung zur Gestaltung von Krisen-PR. Mit dem bedeutenden Diskurs- und dadurch Realitätsgestaltungsmittel Pres...

German PV-producers - an overview. Look back in humility; Photovoltaik Fabriken in Deutschland- eine Uebersicht. Blick zurueck in Demut

Energy Technology Data Exchange (ETDEWEB)

Iken, J.

2001-03-01

Three years are a long period of time in a growing field of industry like photovoltaic power generation. In January 1998, Sonne Wind and Waerme published the first list of German PV producers. The update is presented here. [German] Drei Jahre sind in einer Wachstumsbranche wie der Photovoltaik eine lange Zeit. Im Januar 1998 veroeffentlichte Sonne Wind and Waerme einen Ueberblick ueber die deutschen Photovoltaik-Fabriken. Hoechste Zeit also, die Uebersicht auf den neuesten Stand zu bringen. (orig.)

Old shovels with digital control technology retrofits give new units a run for their money

Energy Technology Data Exchange (ETDEWEB)

Duskey, M.R. [Avtron Manufacturing Inc., Cleveland, OH (United States)

2006-04-15

New digital drive control systems can be retrofitted to shovels and draglines to maximize their performance and keep them running smoothly and reliably. Case histories of successful retrofits are reported. The static drives on a Marion 310 DC drive shovel operating in British Columbia were replaced with Avtron Firing Modules to improve machine availability. Three old Bucyrus 295 M-G set-controlled shovels operated by mines in North Dakota and Labrador which were experiencing excessive downtime were replaced with an Avtron ADD-32DMG field regulator. 2 figs.

EKA 90. aastapäeva juubelinädalale on planeeritud mitmeid üritusi nii peamajas kui osakondades

Index Scriptorium Estoniae

2004-01-01

Kunstööstuskooli tutvustavast näitusest. 29. X audoktorite Udo Kultermanni ja Juhani Pallasmaa loengud ning ümarlaua ettekanded (osalejad loetletud), ümarlauda juhib Jüri Soolep. EKA galeriis valgusinstallatsioonide näitus "Think Lightly", osalevad Sirli Põllumäe, Riho Tiivel, Helis Podnek, Marion Uuspõld, Pent Talvet, Mehis Tiitsar, Merike Rehepapp, Veiko Vaine. Üliõpilaste loomingu väljapanekud. 30. X vilistlaspäev, pidulikul aktusel promoveeritakse audoktoriteks Juhani Pallasmaa, Udo Kultermann, Lars Olof Larsson ja Yrjö Sotamaa. Vabade kunstide osakonna rändnäitus "Ekskursioon" sisekaitseakadeemias

A Comparison of Exogenous Labels for the Histological Identification of Transplanted Neural Stem Cells

Science.gov (United States)

Nicholls, Francesca J.; Liu, Jessie R.; Modo, Michel

2017-01-01

The interpretation of cell transplantation experiments is often dependent on the presence of an exogenous label for the identification of implanted cells. The exogenous labels Hoechst 33342, 5-bromo-2′-deoxyuridine (BrdU), PKH26, and Qtracker were compared for their labeling efficiency, cellular effects, and reliability to identify a human neural stem cell (hNSC) line implanted intracerebrally into the rat brain. Hoechst 33342 (2 mg/ml) exhibited a delayed cytotoxicity that killed all cells within 7 days. This label was hence not progressed to in vivo studies. PKH26 (5 μM), Qtracker (15 nM), and BrdU (0.2 μM) labeled 100% of the cell population at day 1, although BrdU labeling declined by day 7. BrdU and Qtracker exerted effects on proliferation and differentiation. PKH26 reduced viability and proliferation at day 1, but this normalized by day 7. In an in vitro coculture assay, all labels transferred to unlabeled cells. After transplantation, the reliability of exogenous labels was assessed against the gold standard of a human-specific nuclear antigen (HNA) antibody. BrdU, PKH26, and Qtracker resulted in a very small proportion (Exogenous labels can therefore be reliable to identify transplanted cells without exerting major cellular effects, but validation is required. The interpretation of cell transplantation experiments should be presented in the context of the label's limitations. PMID:27938486

German legislation for promotion of renewable energies 2014. Act on feed-in and guaranteed pricing of electricity from renewable energy sources (EEG). Commentary. 7. new rev. ed.; Erneuerbare-Energien-Gesetz 2014. Gesetz fuer den Ausbau erneuerbarer Energien. Kommentar

Energy Technology Data Exchange (ETDEWEB)

Salje, Peter [Hannover Univ. (Germany). Lehrstuhl fuer Zivilrecht und Recht der Wirtschaft

2015-07-01

The EEG is continuously subjected to changes and the permanent center of political discussions. Therefore now already the 7th edition of the EEG comment of the science and practice well recognized and highly recognized author Prof. Dr. Dr. Peter Salje appear. The revision includes, inter alia, further evaluation of the law of electric power produced from renewable energy sources from the EEG of 2012 and the regulations made there under (including Biomass and AusglMechV). The changes associated with the so-called Photovoltaic amendment dated August 2012 and the recent case law since the publication of the previous edition handed down are considered. In particular, the incorporation of the full basic EEG reform in 2014 guarantees the highest topicality. [German] Das EEG ist fortlaufend Neuerungen unterworfen und im staendigen Mittelpunkt der politischen Diskussionen. In nunmehr bereits 7. Auflage erscheint daher der EEG-Kommentar von dem in Wissenschaft und Praxis bestens ausgewiesenen und hoechst anerkannten Autor Prof. Dr. Dr. Peter Salje. Die Neubearbeitung umfasst u.a. die weitere Auswertung des Rechts der Stromerzeugung aus Erneuerbaren Energien aus dem EEG 2012 und den dazu ergangenen Verordnungen (u.a. BiomasseV und AusglMechV). Die mit der sog. Photovoltaik-Novelle vom August 2012 einhergehenden Aenderungen sowie die seit Erscheinen der Vorauflage ergangene Rechtsprechung werden beruecksichtigt. Insbesondere die vollumfaengliche Einarbeitung der grundlegenden EEG-Reform 2014 garantiert hoechste Aktualitaet.

In situ surface-enhanced Raman scattering spectroscopy exploring molecular changes of drug-treated cancer cell nucleus.

Science.gov (United States)

Liang, Lijia; Huang, Dianshuai; Wang, Hailong; Li, Haibo; Xu, Shuping; Chang, Yixin; Li, Hui; Yang, Ying-Wei; Liang, Chongyang; Xu, Weiqing

2015-02-17

Investigating the molecular changes of cancer cell nucleus with drugs treatment is crucial for the design of new anticancer drugs, the development of novel diagnostic strategies, and the advancement of cancer therapy efficiency. In order to better understand the action effects of drugs, accurate location and in situ acquisition of the molecular information of the cell nuclei are necessary. In this work, we report a microspectroscopic technique called dark-field and fluorescence coimaging assisted surface-enhanced Raman scattering (SERS) spectroscopy, combined with nuclear targeting nanoprobes, to in situ study Soma Gastric Cancer (SGC-7901) cell nuclei treated with two model drugs, e.g., DNA binder (Hoechst33342) and anticancer drug (doxorubicin, Dox) via spectral analysis at the molecular level. Nuclear targeting nanoprobes with an assembly structure of thiol-modified polyethylene glycol polymers (PEG) and nuclear localizing signal peptides (NLS) around gold nanorods (AuNRs) were prepared to achieve the amplified SERS signals of biomolecules in the cell nuclei. With the assistance of dark field/fluorescence imaging with simultaneous location, in situ SERS spectra in one cell nucleus were measured and analyzed to disclose the effects of Hoechst33342 and Dox on main biomolecules in the cell nuclei. The experimental results show that this method possesses great potential to investigate the targets of new anticancer drugs and the real-time monitoring of the dynamic changes of cells caused by exogenous molecules.

Anti-Proliferative and Apoptotic Effects of Beta-Ionone in Human Leukemia Cell Line K562

Directory of Open Access Journals (Sweden)

Zohreh Faezizadeh

2016-06-01

Full Text Available Background Beta-ionone is an aroma compound found in the Rosaceae family. Some evidence supported that beta-ionone has a great potential for cancer prevention. To date, the anti-proliferative and apoptotic effects of beta-ionone in human leukemia cell line K562 were not studied. Objectives Hence, we investigated whether beta-ionone could inhibit cell growth and induce apoptosis in the K562 cells. Materials and Methods In this experimental study, human leukemia cell line K562 was cultured and anti-proliferation effect of beta-ionone with different doses (25 - 400 µm at different times (24 - 96 hours on treated cells was evaluated by the MTT assay. To determine apoptosis rate, the Hoechst 33342 staining and flow cytometry was performed. Results The MTT assay showed that beta-ionone inhibited proliferation of K562 cells in a dose-dependent manner significantly (P = 0.0008. Moreover, the increased apoptotic rate was found after incubation of K562 cells with 200 µm beta-ionone. The Hoechst staining and flow cytometry analysis indicated that beta-ionone could increase apoptosis of K562 cells in a dose-dependent manner. Conclusions The results demonstrated that beta-ionone has anti-proliferative and apoptotic effects on K562 cells, and in the future may be used in the treatment of some leukemia sub-types.

Bibliometrics of intraoperative radiotherapy. Analysis of technology, practice and publication tendencies

Energy Technology Data Exchange (ETDEWEB)

Sole, Claudio V. [Instituto de Radiomedicina, Service of Radiation Oncology, Santiago (Chile); School of Medicine Complutense University, Madrid (Spain); Institut Gustave Roussy, Department of Radiation Oncology, Paris (France); Calvo, Felipe A. [School of Medicine Complutense University, Madrid (Spain); Hospital General Universitario Gregorio Maranon, Department of Oncology, Madrid (Spain); Hospital General Universitario Gregorio Maranon, Institute for Sanitary Research, Madrid (Spain); Ferrer, Carlos [Hospital Provincial de Castellon, Institute of Oncology, Castellon de la Plana (Spain); Cardenal Herrera-CEU University, School of Medicine, Castellon de la Plana (Spain); Pascau, Javier [Hospital General Universitario Gregorio Maranon, Institute for Sanitary Research, Madrid (Spain); Marsiglia, Hugo [Institut Gustave Roussy, Department of Radiation Oncology, Paris (France)

2014-12-15

wurden. Es wurde eine bibliometrische Suche nach IORT-Publikationen vorgenommen, die in der Pubmed-Datenbank zwischen 1997 und 2013 veroeffentlicht wurden. Als Mengenindikator verwendeten wir die Publikationsrate und als Qualitaetsindikator den ''2012 Citations Index Impact Factor''. Die Arbeiten wurden ausserdem geordnet nach Studientyp, wissenschaftlichem Fokus, Tumorentitaet, Art der Zeitschrift sowie Erscheinungsjahr. Um die Unterschiede zwischen den Mittelwerten der untersuchten Gruppen zu bestimmen, wurde eine Einwegvarianzanalyse (ANOVA) durchgefuehrt. Von insgesamt 207 Fachzeitschriften wurde signifikant haeufiger in jenen der Chirurgie (n = 399; 41 %) und der Strahlentherapie (n = 273; 28 %; p < 0,01) publiziert, wobei der hoechste Impactfaktor in Fachjournalen der klinischen Onkologie erzielt wurde (p < 0,01). Die meisten Veroeffentlichungen bezogen sich auf retrospektive Kohortenstudien (n = 622; 64 %), gefolgt von Uebersichtsarbeiten (n = 204; 21 %; p < 0,001). Zu klinischen Ergebnissen nach spezifischer Tumortherapie erschienen im Vergleich zu anderen Themen die zahlreichsten Arbeiten (n = 661; 68 %, p < 0,001); hier wurde auch der hoechste mittlere Impactfaktor erreicht (p < 0,01). Am haeufigsten fanden sich Veroeffentlichungen ueber gastrointestinale Tumoren (n = 456; 47 %; p < 0,001); im Bereich gynaekologischer Tumoren und Mammakarzinome wurde dagegen der hoechste mittlere Impactfaktor erzielt (p < 0,01). Es war festzustellen, dass die wissenschaftliche Produktivitaet internationaler IORT-Expertengruppen bestaendig und nachhaltig ist. Die meisten Veroeffentlichungen erschienen in chirurgischen und radioonkologischen Fachzeitschriften. Der hoechste Impactfaktor wurde in Fachzeitschriften mit internistisch-onkologischer Ausrichtung erzielt. (orig.)

O paradoxo na Teoria da Argumentação na Língua: uma questão linguístico-argumentativa = The paradox in Language Argumentation Theory: a linguisticargumentative issue

Directory of Open Access Journals (Sweden)

Rosi Ana Grégis

2009-04-01

Full Text Available Na Teoria da Argumentação na Língua (TAL, o conceito de paradoxo estáligado à hipótese de que ele pode ser definido em termos puramente linguísticos. Nessa definição, entende-se que há uma argumentação estruturalmente relacionada a algumas expressões das línguas naturais. Esse conceito indica que a argumentação na língua estáligada a algumas palavras e às suas argumentações internas. Por meio da análise de encadeamentos paradoxais, definidos por Oswald Ducrot e Marion Carel (2001, na TAL, este artigo tem o propósito de mostrar que alguns tipos de paradoxo podem ser explicados em termos puramente linguísticos, sem serem considerados contraditórios, absurdos ou, atémesmo, de difícil compreensão.In the Language Argumentation Theory (TAL, the concept of paradox is linked to the hypothesis that it can be defined in merely linguistic terms. In this definition, it is understood that there is a structural argumentation related to some expressions in natural languages. This concept indicates that language is associated to somewords and their internal argumentations. Through the analysis of paradoxical enchainments, defined by Oswald Ducrot and Marion Carel (2001, in the Language Argumentation Theory, this article aims to confirm that some kinds of paradox can be explained in strictly linguistic terms, without being considered something contradictory,absurd or even difficult to understand.

La production de l'impartialité: la construction du discours universel à partir de la perspective journalistique

OpenAIRE

Miguel, Luis Felipe; Tokarski, Flávia Millena Biroli

2010-01-01

Neste trabalho, procuramos indicar abordagens teóricas e hipóteses alternativas para a crítica à noção de imparcialidade no jornalismo. A proposta consiste em uma abordagem crítica a posições teóricas que legitimam o jornalismo como fiador do pluralismo político. A partir das noções de perspectiva e conhecimento situado, trabalhadas por Iris Marion Young e Nancy Fraser, estabelecemos um deslocamento em relação à crítica liberal pluralista que mantém a imparcialidade como um valor-guia. Com ba...

Argumentação linguística, enunciação e polifonia = Linguistic argumentation, utterance and polyphony

OpenAIRE

Gomes, Neiva Maria Tebaldi

2016-01-01

Este artigo faz uma incursão pela teoria polifônica apresentada por Oswald Ducrot em 1984, em O Dizer e o Dito, atualizada posteriormente por Marion Carel e Oswald Ducrot, e em constante revisão em estudos de Carel. Essa teoria é criada em oposição ao pressuposto da unicidade do sujeito falante. A partir de um estudo teórico-reflexivo, buscam-se, na teoria polifônica decorrente da Teoria da Argumentação na Língua, elementos que contribuam para a explicitação dos sentidos produzidos em textos ...

A New "Shield of the Weak": Continued Paternalism of Domestic Violence Services in Uruguay.

Science.gov (United States)

Bloom, Allison

2018-03-01

Drawing on ethnographic and historical research, this article illuminates the limitations of the Uruguayan domestic violence services system. In spite of how advocates in Uruguay successfully used a human rights platform to secure legislation and services, this system now faces significant critique. Using Iris Marion Young's work on the "logic of masculinist protection" and historical parallels in Uruguay's welfare system, I discuss how a paternalistic approach may be to blame. I highlight how this paternalism contributes to the paternalism that problematically underlies gendered violence-reinforcing rather than addressing oppressive ideologies and structures that impede improving conditions for women.

Ergonomics for users of VDU screens

CERN Multimedia

2003-01-01

(course in French) A 1/2 day course to promote awareness of the risks incurred by bad posture when working on a computer screen: backache, tired eyes, wrist pains.... and advice on avoiding them. Next course in September. Costs are covered by TIS, Clemente Pilly and Ribordy Marion from « PM postures » will present the course. Registration is obligatory. For more information and to enrol on this course go to the safety section of Human Resources Training and Development web pages, or contact : Ana-Paula Bernardes/TIS-GS (71385) Ana-Paula.Bernardes@cern.ch

Ergonomics for users of VDU screens

CERN Multimedia

2003-01-01

(course in French) A 1/2 day course to promote awareness of the risks incurred by bad posture when working on a computer screen: backache, tired eyes, wrist pains.... and advice on avoiding them. Next course in September. Costs are covered by TIS, Clemente Pilly and Ribordy Marion from « PM postures » will present the course. Registration is obligatory. For more information and to enrol on this course go to the safety section of Human Resources Training and Development web pages, or contact : /TIS-GS (71385) or e-mail Ana-Paula.Bernardes@cern.ch

Geologic map of the St. Joe quadrangle, Searcy and Marion Counties, Arkansas

Science.gov (United States)

Hudson, Mark R.; Turner, Kenzie J.

2009-01-01

This map summarizes the geology of the St. Joe 7.5-minute quadrangle in the Ozark Plateaus region of northern Arkansas. Geologically, the area lies on the southern flank of the Ozark dome, an uplift that exposes oldest rocks at its center in Missouri. Physiographically, the St. Joe quadrangle lies within the Springfield Plateau, a topographic surface generally held up by Mississippian cherty limestone. The quadrangle also contains isolated mountains (for example, Pilot Mountain) capped by Pennsylvanian rocks that are erosional outliers of the higher Boston Mountains plateau to the south. Tomahawk Creek, a tributary of the Buffalo River, flows through the eastern part of the map area, enhancing bedrock erosion. Exposed bedrock of this region comprises an approximately 1,300-ft-thick sequence of Ordovician, Mississippian, and Pennsylvanian carbonate and clastic sedimentary rocks that have been mildly deformed by a series of faults and folds. The geology of the St. Joe quadrangle was mapped by McKnight (1935) as part of a larger area at 1:125,000 scale. The current map confirms many features of this previous study, but it also identifies new structures and uses a revised stratigraphy. Mapping for this study was conducted by field inspection of numerous sites and was compiled as a 1:24,000-scale geographic information system (GIS) database. Locations and elevations of sites were determined with the aid of a global positioning satellite receiver and a hand-held barometric altimeter that was frequently recalibrated at points of known elevation. Hill-shade-relief and slope maps derived from a U.S. Geological Survey 10-m digital elevation model as well as U.S. Geological Survey orthophotographs from 2000 were used to help trace ledge-forming units between field traverses within the Upper Mississippian and Pennsylvanian part of the stratigraphic sequence. Strikes and dips of beds were typically measured along stream drainages or at well-exposed ledges. Beds dipping less than 2 degrees are shown as horizontal. Structure contours constructed on the base of the Boone Formation were hand drawn based on elevations of control points on both lower and upper contacts of the Boone Formation as well as other limiting information on their maximum or minimum elevations.

DIGITAL FLOOD INSURANCE RATE MAP DATABASE, MARION COUNTY, TENNESSEE AND INCORPORATED AREAS

Data.gov (United States)

Federal Emergency Management Agency, Department of Homeland Security — The Digital Flood Insurance Rate Map (DFIRM) Database depicts flood risk information and supporting data used to develop the risk data. The primary risk...

Diagnosing Guerilla Warfare: Was William Clarke Quantrill Missouri’s Francis Marion?

Science.gov (United States)

2010-04-28

the Military Struggle for American Independence. rev ed. A1m Arbor: University of Michigan Press, 1990. Sutherland , Daniel, ed. Guerillas, Unionists...Directed by Ray Emigh. Universal Intemational Pictures, 1950. The Outlaw Josey Wales. With Clint Eastwood and Sondra Locke. Directed by Clint ...raiding Jayhawkers. 87 The Outlaw Josey Wales. With Clint Eastwood and Sondra Locke. Directed by Clint ·Eastwood. Warner Studios, 1976. In one of

Cd-tolerant Suillus luteus: A fungal insurance for pines exposed to Cd

International Nuclear Information System (INIS)

Krznaric, Erik; Verbruggen, Nathalie; Wevers, Jan H.L.; Carleer, Robert; Vangronsveld, Jaco; Colpaert, Jan V.

2009-01-01

Soil metal pollution can trigger evolutionary adaptation in soil-borne organisms. An in vitro screening test showed cadmium adaptation in populations of Suillus luteus (L.: Fr.) Roussel, an ectomycorrhizal fungus of pine trees. Cadmium stress was subsequently investigated in Scots pine (Pinus sylvestris L.) seedlings inoculated with a Cd-tolerant S. luteus, isolated from a heavy metal contaminated site, and compared to plants inoculated with a Cd-sensitive isolate from a non-polluted area. A dose-response experiment with mycorrhizal pines showed better plant protection by a Cd-adapted fungus: more fungal biomass and a higher nutrient uptake at high Cd exposure. In addition, less Cd was transferred to aboveground plant parts. Because of the key role of the ectomycorrhizal symbiosis for tree fitness, the evolution of Cd tolerance in an ectomycorrhizal partner such as S. luteus can be of major importance for the establishment of pine forests on Cd-contaminated soils. - The evolutionary adaptation for higher Cd tolerance in Suillus luteus, an ectomycorrhizal fungus, is of major importance for the amelioration of Cd toxicity in pine trees exposed to high Cd concentrations.

Cd-tolerant Suillus luteus: A fungal insurance for pines exposed to Cd

Energy Technology Data Exchange (ETDEWEB)

Krznaric, Erik [Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590 Diepenbeek (Belgium); Verbruggen, Nathalie [Laboratoire de Physiologie et de Genetique Moleculaire des Plantes, Universite Libre de Bruxelles, Campus Plaine, CP242, Bd du Triomphe, 1050 Brussels (Belgium); Wevers, Jan H.L. [Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590 Diepenbeek (Belgium); Carleer, Robert [Laboratory of Applied Chemistry, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590 Diepenbeek (Belgium); Vangronsveld, Jaco [Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590 Diepenbeek (Belgium); Colpaert, Jan V., E-mail: jan.colpaert@uhasselt.b [Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590 Diepenbeek (Belgium)

2009-05-15

Soil metal pollution can trigger evolutionary adaptation in soil-borne organisms. An in vitro screening test showed cadmium adaptation in populations of Suillus luteus (L.: Fr.) Roussel, an ectomycorrhizal fungus of pine trees. Cadmium stress was subsequently investigated in Scots pine (Pinus sylvestris L.) seedlings inoculated with a Cd-tolerant S. luteus, isolated from a heavy metal contaminated site, and compared to plants inoculated with a Cd-sensitive isolate from a non-polluted area. A dose-response experiment with mycorrhizal pines showed better plant protection by a Cd-adapted fungus: more fungal biomass and a higher nutrient uptake at high Cd exposure. In addition, less Cd was transferred to aboveground plant parts. Because of the key role of the ectomycorrhizal symbiosis for tree fitness, the evolution of Cd tolerance in an ectomycorrhizal partner such as S. luteus can be of major importance for the establishment of pine forests on Cd-contaminated soils. - The evolutionary adaptation for higher Cd tolerance in Suillus luteus, an ectomycorrhizal fungus, is of major importance for the amelioration of Cd toxicity in pine trees exposed to high Cd concentrations.

Autophagy involved in resveratrol increased radiosensitivity in glioma stem cells

International Nuclear Information System (INIS)

Long Linmei; Zhang Qingqing; Yang Neng; Ji Wenjun; Song Yunzhen; Zhao Jianghu; Liang Zhongqin

2012-01-01

Objective: To investigate the effect of Resveratrol combined with X-ray on radiosensitivity in glioma stem cells. Methods: The proliferation inhibition of glioma stem cells induced by X-rays and Resveratrol was assessed with MTT assay. The activation of proapoptotic effect was characterized by Hoechst 33258 stain. MDC stain and Western blot analysis were used to analyze the autophagy mechanism in X-rays-induced death of glioma stem cells. Results: MTT assay indicated that X-rays and Resveratrol decreased the viability of glioma stem cells (P<0.05); we found the proliferative inhibition of glioma stem cells was declined when we used 3-MA to inhibit autophagy(P<0.05). When the cells were treated by the Resveratrol and x-rays, their spherical shape were changed. Apoptosis was induced in glioma stem cells by combined X-rays and Resveratrol as detected by Hoechst 33258 staining. In addition, autophagy was induced in glioma stem cells in the combined treatment group as detected by MDC staining. Western blotting showed that Bcl-2 expression was decreased. in the combined treatment group (P<0.01), and the LC3-Ⅱ expression was increased in the combined treatment group (P<0.01). Conclusion: Resveratrol can increased the radiation sensitivity of glioma stem cells, the apoptosis and autophagy was induced in the glioma stem cells in the combined treatment X-rays and Resveratrol. Our results suggest that autophagy plays an essential role in the regulation of radiosensitization of glioma stem cells. (authors)

Effects of intravenous administration of bone marrow stromal stem cells on cognitive impairment of the whole-brain irradiated rat models

International Nuclear Information System (INIS)

Ding Weijun; Wang Jianhua; Zhu Min; Chen Baoguo; Wang Yang

2007-01-01

Objective: To explore the effect of intravenous infusion of bone marrow stromal stem cells(MSCs) on cognitive function of rats after whole brain irradiation. Methods: MSCs were isolated and cultured from adult rats. After Sprague-Dawly female rats were anaesthetized with chloral hydrate, their whole cerebrum was irradiated with a single dose of 20 Gy by 6 MV X-ray. Seven days after irradiation, 4 x 106 Hoechst33342-1abelled MSCs were intravenously injected into the tail vein of these rats. Four and 8 weeks after transplantation, the learning and memorizing ability was measured with the Y maze test. Immunohistochemical method was used to identify MSCs or ceils derived from MSCs in the brain. Results: The learning and memorizing ability of irradiation groups were significantly different from that of normal control group (P < 0.01). Significant improvement of cognitive impairment was observed in rats treated with MSCs at 4 and 8 weeks after transplantation as compared with the controll groups (P<0.05). This showed that the MSCs survived and were localized to the brain tissue. The number of Hoechst33342 immunohistofluorescence positive cells and double-immunostaining cells significantly decreased in 8 weeks group as compared with the 4 weeks group. Conclusion: Marrow stromal stem cells delivered to the irradiation brain tissue through intravenous route improve the cognitive impairment after whole brain irradiation. These cells may survive and differentiate in the brain tissue of irradiated rats. (authors)

The effect of β-ionone on telomerase activity in the human leukemia cell line K562

Directory of Open Access Journals (Sweden)

Zohreh Faezizadeh

2015-06-01

Full Text Available Background: Telomerase is highly activated in most human cancer cells, therefore, its inhibition has been proposed as a novel and promising strategy for cancer therapy. Many plant-derived anticancer agents act through inhibition of telomerase activity and induction of apoptosis. β-ionone, a carotenoid compound isolated from Roseaceae, has been reported to possess anticancer properties. The present study was undertaken to examine the mechanism of β-ionone-induced apoptosis in human leukemia cell line K562 with special emphasis on its role in telomerase inhibition. Method: In this study the anti-proliferation effect of β-ionone on K562 cells was evaluated by MTT assay. Apoptosis rate was detected by Hoechst staining and flow cytometry analysis. Telomerase activity was measured by (TRAP ELISA assay. Results: Exposure of K562 cells to β-ionone caused a dose-dependent decrease in proliferation. Flow cytometry analysis and Hoechst staining showed that percentage of apoptotic cells markedly increased with an increase in β-ionone concentration. Compared to control cells, treatment of K562 cells with β-ionone resulted in a significant decrease of telomerase activity. Moreover, a positive correlation was detected between telomerase inhibition and apoptosis induction in the treated K562 cells. Conclusion: Based on these results, β-ionone is an appropriate candidate for inhibiting telomerase activity in K562 cells. Therefore, it may be utilized as a novel drug against some leukemia cell lines.

Modification of radiation damage in mouse lung by DNA-binding radioprotectors

International Nuclear Information System (INIS)

Budd, R.; D'Abrew, S.; Coultas, P.; Martin, R.F.

1996-01-01

Full text: The limited diffusion of Hoechst 33342 through cell layers, which has been exploited in mapping the location of cells in multi-cellular spheroids, and in vivo, reflects a general characteristic of DNA-ligands. This property may confer special advantages on DNA-binding radioprotectors in the context of radiotherapy, where it is important to minimise delivery of the radioprotector to the tumour. For example, one might expect limited diffusion to capillaries and systemic uptake following topical application to epithelial cells, which can be dose-limiting tissues in radiotherapy. These potential applications will require delivery of sufficient concentrations of the DNA-binding radioprotectors to cells in vivo. In this context, the findings of Young and Hill, who could not detect any radioprotective effect in an in vivo setting, is of concern. We have re-examined this question by investigating radioprotection in the mouse lung model. A single intravenous injection of Hoechst 33342 (80mg/kg) given 30min prior to the lung irradiation, extends the radiation dose required for death in 50% of mice at 16 weeks post irradiation, from 19Gy to 23Gy (ie: a DMF of 1.2). This is similar to the extent of radioprotection reported by Travis et al for WR2721 (300 mg/kg) in this model. These results auger well for the potential of the more potent radioprotectors, and indeed preliminary experiments with methylproamine in the mouse lung model suggests a DMF of 1.35

Physics and Materials Science of High Temperature Superconductors

Science.gov (United States)

1989-08-26

30 M. Nikolo: Effect of Texture and Density on Grain Coupling of Sintered Y-Ba-Cu-O Superconductors 11:30 - 12:00 A. SzAsz : The Symmetries and High...The Hall coefficient is always positive and obeys the RH (x T- 1 law . The I Hall mobility p2-I2 < I cm2/V.S. At T < Tc, the thermoelectric power equals...461561 80-6550 Prof. A. Szasz PP Dr. Martin Schwarz PP Eotvos University Angewandte Physik Muzeum Krt. 6-8 Hoechst AG H-1088 Budapest Hungary Gebaeude

Argumentação linguística, enunciação e polifonia

OpenAIRE

Gomes, Neiva Maria Tebaldi; Centro Universitário Ritter dos Reis/Laureate

2016-01-01

Este artigo faz uma incursão pela teoria polifônica apresentada por Oswald Ducrot em 1984, em O Dizer e o Dito, atualizada posteriormente por Marion Carel e Oswald Ducrot, e em constante revisão em estudos de Carel. Essa teoria é criada em oposição ao pressuposto da unicidade do sujeito falante. A partir de um estudo teórico-reflexivo, buscam-se, na teoria polifônica decorrente da Teoria da Argumentação na Língua, elementos que contribuam para a explicitação dos sentidos produzidos em textos ...

Asteroid Lightcurve Analysis at the Palmer Divide Observatory: 2008 December - 2009 March

Science.gov (United States)

Warner, Brian D.

2009-07-01

Lightcurves for 34 asteroids were obtained at the Palmer Divide Observatory from 2008 December through 2009 March: 91 Aegina, 261 Prymno, 359 Georgia, 402 Chloe, 497 Iva, 506 Marion, 660 Crescentia, 691 Lehigh, 731 Sorga, 779 Nina, 802 Epyaxa, 908 Buda, 1015 Christa, 1518 Rovaniemi, 1600 Vyssotsky, 1656 Suomi, 2000 Herschel, 2735 Ellen, 3169 Ostro, 3854 George, 3940 Larion, (5558) 1989 WL2, (5747) 1991 CO3, 6517 Buzzi, (11304) 1993 DJ, (22195) 3509 P-L, (26383) 1999 MA2, (29780) 1999 CJ50, (45878) 2000 WX29, (45898) 2000 XQ49, (76800) 2000 OQ35, (76929) 2001 AX34, (87343) 2000 QH25, and (207398) 2006 AS2.

Usurper la médiévité : stratégies archaïsantes des réécritures arthuriennes contemporaines

OpenAIRE

Besson, Anne

2014-01-01

Les réécritures arthuriennes contemporaines constituent un énorme corpus romanesque regroupant plusieurs dizaines de vastes cycles et de nombreux succès commerciaux, comme Les Dames du Lac de Marion Zimmer Bradley, les ensembles de Bernard Cornwell ou Stephen Lawhead – un corpus d’une grande homogénéité thématique (on y redit toujours la même histoire à peu près, avec les mêmes personnages à peu près), mais d’une identité générique en revanche hétérogène, fluctuant entre roman historique et f...

Neuroprotective effect of the natural iron chelator, phytic acid in a cell culture model of Parkinson's disease

International Nuclear Information System (INIS)

Xu Qi; Kanthasamy, Anumantha G.; Reddy, Manju B.

2008-01-01

Disrupted iron metabolism and excess iron accumulation has been reported in the brains of Parkinson's disease (PD) patients. Because excessive iron can induce oxidative stress subsequently causing degradation of nigral dopaminergic neurons in PD, we determined the protective effect of a naturally occurring iron chelator, phytic acid (IP6), on 1-methyl-4-phenylpyridinium (MPP + )-induced cell death in immortalized rat mesencephalic/dopaminergic cells. Cell death was induced with MPP + in normal and iron-excess conditions and cytotoxicity was measured by thiazolyl blue tetrazolium bromide (MTT assay) and trypan blue staining. Apoptotic cell death was also measured with caspase-3 activity, DNA fragmentation, and Hoechst nuclear staining. Compared to MPP + treatment, IP6 (30 μmol/L) increased cell viability by 19% (P + treatment was decreased by 55% (P < 0.01) and 52% (P < 0.05), respectively with IP6. Cell survival was increased by 18% (P < 0.05) and 42% (P < 0.001) with 30 and 100 μmol/L of IP6, respectively in iron-excess conditions. A 40% and 52% (P < 0.001) protection was observed in caspase-3 activity with 30 and 100 μmol/L IP6, respectively in iron-excess condition. Similarly, a 45% reduction (P < 0.001) in DNA fragmentation was found with 100 μmol/L IP6. In addition, Hoechst nuclear staining results confirmed the protective effect of IP6 against apoptosis. Similar protection was also observed with the differentiated cells. Collectively, our results demonstrate a significant neuroprotective effect of phytate in a cell culture model of PD

Cadmium telluride quantum dots induce apoptosis in human breast cancer cell lines.

Science.gov (United States)

Naderi, Saeed; Zare, Hakimeh; Taghavinia, Nima; Irajizad, Azam; Aghaei, Mahmoud; Panjehpour, Mojtaba

2018-05-01

Semiconductor quantum dots (QDs), especially those containing cadmium, have undergone marked improvements and are now widely used nanomaterials in applicable biological fields. However, great concerns exist regarding their toxicity in biomedical applications. Because of the lack of sufficient data regarding the toxicity mechanism of QDs, this study aimed to evaluate the cytotoxicity of three types of QDs: CdTe QDs, high yield CdTe QDs, and CdTe/CdS core/shell QDs on two human breast cancer cell lines MDA-MB468 and MCF-7. The breast cancer cells were treated with different concentrations of QDs, and cell viability was evaluated via MTT assay. Hoechst staining was applied for observation of morphological changes due to apoptosis. Apoptotic DNA fragmentation was visualized by the agarose gel electrophoresis assay. Flow cytometric annexin V/propidium iodide (PI) measurement was used for apoptosis detection. A significant decrease in cell viability was observed after QDs treatment ( p < 0.05). Apoptotic bodies and chromatin condensation was observed by Hoechst staining. DNA fragmentation assay demonstrated a DNA ladder profile in the exposed cells and also annexin V/PI flow cytometry confirmed apoptosis in a dose-dependent manner. Our results revealed that CdTe, high yield CdTe, and CdTe/CdS core/shell QDs induce apoptosis in breast cancer cell lines in a dose-dependent manner. This study would help realizing the underlying cytotoxicity mechanism, at least partly, of CdTe QDs and may provide information for the development of nanotoxicology and safe use of biological applications of QDs.

OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation

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Lu Liu

2017-01-01

Full Text Available Introduction. Infection and apoptosis are combined triggers for inflammation in dental tissues. Octamer-binding transcription factor 4-B1 (OCT4B1, a novel spliced variant of OCT4 family, could respond to the cellular stress and possess antiapoptotic property. However, its specific role in dental pulpitis remains unknown. Methods. To investigate the effect of OCT4B1 on inflammation of dental pulp cells (DPCs, its expression in inflamed dental pulp tissues and DPCs was examined by in situ hybridization, real-time PCR, and FISH assay. OCT4B1 overexpressed DPCs model was established, confirmed by western blot and immunofluorescence staining, and then stimulated with Lipopolysaccharide (LPS. Apoptotic rate was determined by Hoechst/PI staining and FACS. Cell survival rate was calculated by CCK8 assay. Results. In situ hybridization, real-time PCR, and FISH assay revealed that OCT4B1 was extensively expressed in inflamed dental pulp tissues and DPCs with LPS stimulation. Western blot and immunofluorescence staining showed the expression of OCT4B1 and OCT4B increased after OCT4B1 transfection. Hoechst/PI staining and FACS demonstrated that less red/blue fluorescence was detected and apoptotic percentage decreased (3.45% after transfection. CCK8 demonstrated that the survival rate of pCDH-OCT4B1-flag cells increased. Conclusions. OCT4B1 plays an essential role in inflammation and apoptosis of DPCs. OCT4B might operate synergistically with OCT4B1 to reduce apoptosis.

Kas riik võib kliimamuutuste tõttu kaduda? Rahvusvahelise õiguse probleemid / Marion Ross

Index Scriptorium Estoniae

Ross, Marion, 1988-

2013-01-01

Riigi staatusest, kui tema territoorium jääb kliima soojenemise tõttu püsivalt vee alla. Võimalikud lahendused riigi staatuse säilitamiseks - territooriumi ostmisest, rentimisest ja tehissaartele ümberasumisest. Riikide õiguste ja kohustuste konventsioonist (Montevideo, 1933). Artikkel põhineb autori 2013. a. kevadel Tartu Ülikooli õigusteaduskonnas kaitstud magistritööl "Kas riik võib kliima soojenedes kaduda? Rahvusvahelis-õiguslikud probleemid"

Mature Erythrocytes of Iguana iguana (Squamata, Iguanidae) Possess Functional Mitochondria.

Science.gov (United States)

Di Giacomo, Giuseppina; Campello, Silvia; Corrado, Mauro; Di Giambattista, Livia; Cirotti, Claudia; Filomeni, Giuseppe; Gentile, Gabriele

2015-01-01

Electron microscopy analyses of Iguana iguana blood preparations revealed the presence of mitochondria within erythrocytes with well-structured cristae. Fluorescence microscopy analyses upon incubation with phalloidin-FITC, Hoechst 33342 and mitochondrial transmembrane potential (Δψm)-sensitive probe MitoTracker Red indicated that mitochondria i) widely occur in erythrocytes, ii) are polarized, and iii) seem to be preferentially confined at a "perinuclear" region, as confirmed by electron microscopy. The analysis of NADH-dependent oxygen consumption showed that red blood cells retain the capability to consume oxygen, thereby providing compelling evidence that mitochondria of Iguana erythrocytes are functional and capable to perform oxidative phosphorylation.

Uptake of macromolecules by cercariae during skin penetration and transformation to schistosomula (Schistosoma mansoni

Directory of Open Access Journals (Sweden)

Joyce Thornhill

2010-07-01

Full Text Available Here, we observed the uptake of membrane-impermeant molecules by cercariae as they penetrate the skin and are transformed into schistosomula. We propose that membrane-impermeant molecules, Lucifer Yellow, Propidium iodide and Hoechst 33258 enter the parasite through both thenephridiopore and the surface membrane and then diffuse throughout the body of the parasite. We present a hypothesis that the internal cells of the body of the schistosomulum represent a new host-parasite interface, at which skin-derived growth factors may stimulate receptors on internal membranes during transformation of the cercariae into the schistosomulum.

Eiffel tower of the Siegerland region; Eiffelturm des Siegerlandes

Energy Technology Data Exchange (ETDEWEB)

Dreifert, M.

2001-01-12

The first wind power system constructed at Siegen-Wittgenstein is the highest ever, with a hub height of 117 m for the Vesta V66. The impressive steel trelliswork power is also the first wind power system granted a special license by the state of Nordrhein-Westfalen for construction in a protected forest site. [German] Die erste Windkraftanlage in Siegen-Wittenstein ist zugleich die derzeit hoechste ueberhaupt: Auf 117 Meter befindet sich die Nabe der Vestas V66. Das imposante Gittermast-Bauwerk ist aber auch die erste Windkraftanlage, fuer die das Land Nordrhein-Westfalen eine Waldumwandlungsgenehmigung erteilte. (orig.)

Studies of cellular damage induced by X-rays and visible light

International Nuclear Information System (INIS)

Christensen, T.; Kinn, G.; Reitan, J.B.

1989-01-01

DNA-damage in cells has been studied by use of spectrophotometry and fluorometry. The method is based on the differential fluorescence quantum yield of the fluorochrome Hoechst 33258 when bound to single and double stranded DNA, respectively. DNA-damage by doses of X-rays below 2 Gy was clearly detectable. Blue light from phototherapy lamps induced DNA-damage in human TMG-1 glioblastoma, but no significant effect could be observed after irradiation with green lamps. In the presence of bilirubin the amount of DNA-damage was increased, notably at high bilirubin concentration and by blue light. 9 refs; 12 figs

Anisotropic tomography of the Indian continent and the geodynamic role of its keel

Science.gov (United States)

Montagner, J. P.; Maurya, S.; Sibrant, A.; Davaille, A.; Stutzmann, E.; Kumar, R.; Jean, B.

2017-12-01

The Indian moved at an exceptional high rate (18-20cm/year) after the birth of La Réunion hotspot, ≈65Ma ago and the Deccan volcanic province before the collision with the Asian continent. Other older plumes Marion, Kerguelen located in the Indian ocean are also associated with very fast plate motion. We present a high-resolution 3D anisotropic model of the Indian plate region down to 300 km depth, obtained by inverting a new massive database of surface-wave observations. The Rayleigh and Love wave dispersion measurements along 14,000 paths are made in a broad frequency range (16-250s). Our estimates of the depth to the Lithosphere-Asthenosphere Boundary (LAB) derived from seismic velocity Vsv variations at depth reveal large variations (120-250 km) beneath the different cratonic blocks. A low velocity layer associated with the Mid-lithospheric discontinuity is present when the root of the lithosphere is deep. This extensive anisotropic tomographic investigation of the Indian continent displays an almost north-south keel, 600km long and 300km wide, down to 250km depth. The keel is characterized by fast velocities, smaller than average radial and azimuthal anisotropies. The distribution of azimuthal anisotropy defines the flow lines around the keel, and, at the LAB, coincides with the APM direction of the Indian plate. The fast axis azimuths at 250 km depth are also in accordance with the results from SK(K)S splitting. Such a keel could probably perturb plume-induced flow in the asthenosphere. To determine the influence of such a keel on the interaction of India with several mantle plumes (Marion, Crozet, Kerguelen, La Réunion), we used laboratory experiments. Some preliminary results will be presented on the comparison between the laboratory flowlines and the direction of seismic anisotropy.

Nuevas fuentes de subjetivación: Hacia una teoría política del cuerpo

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Martínez Ramírez, Máriam

2009-06-01

Full Text Available The modernity establishes the basis of subjectivity from the Cartesian dualist separation of the mind as the active consciousness and subject and the body as a passive material object. This essay takes as standpoint the political philosophy of Iris Marion Young in relation to her phenomenological writings. Thus, it accepts a metaphysical approach on the body as lived body and situation which constructs subjectivity for any human being. It argues that the author gives to the body the ontological status that Cartesian tradition attributes to the consciousness alone. The essay reflects on different aspects of the social consequences of this tradition, and the new horizons that several feminist studies give in many significant ways to the embodiment political theory.

La modernidad instaura las bases de la subjetividad a partir de la separación cartesiana que distingue entre la mente, como conciencia activa del sujeto, y el cuerpo, como objeto material pasivo de esa conciencia activa. Este artículo toma como punto de partida la filosofía política de Iris Marion Young, en relación a sus escritos sobre fenomenología, corporalidad y género, para aceptar un enfoque metafísico sobre el cuerpo como significación de la experiencia vivida y situación que conforma el proceso de subjetivación de todo ser humano. De esta manera, la autora confiere al cuerpo el estatus ontológico que aquella tradición cartesiana confiere a la sola conciencia. Sobre las consecuencias sociales del peso de esta tradición metafísica cartesiana, y los nuevos horizontes que ofrecen algunos estudios feministas en relación con el pensamiento de la encorporación («embodiment thinking» indaga este artículo.

Laser-induced ionization of Na vapor

International Nuclear Information System (INIS)

Wu, R.C.Y.; Judge, D.L.; Roussel, F.; Carre, B.; Breger, P.; Spiess, G.

1982-01-01

The production of Na 2 + ions by off-resonant laser excitation in the 5800-6200A region mainly results from two-photon absorption by the Na 2 molecule to highly excited gerade states followed by (a) direct ionization by absorbing a third photon or (b) coupling to the molecular Na 2 D 1 PIμ Rydberg state which is subsequently ionized by absorbing a third photon. This mechanism, i.e., a two-photon resonance three photon ionization process, explains a recent experimental observation of Roussel et al. It is suggested that the very same mechanism is also responsible for a similar observation reported by Polak-Dingels et al in their work using two crossed Na beams. In the latter two studies the laser-induced associative ionization processes were reported to be responsible for producing the Na 2 + ion. From the ratio of molecular to atomic concentration in the crossed beam experiment of Polak-Dingels et al we estimate that the cross section for producing Na 2 + through laser-induced associative ionization is at least four orders of magnitude smaller than ionization through the two-photon resonance three photon ionization process in Na 2 molecules

A rare case of methimazole-induced cholestatic jaundice in an elderly man of Asian ethnicity with hyperthyroidism: A case report.

Science.gov (United States)

Ji, Hongjian; Yue, Feng; Song, Jianxiang; Zhou, Xiaohua

2017-12-01

Methimazole is an antithyroid drug that is widely used for the treatment of hyperthyroidism. As an inhibitor of the enzyme thyroperoxidase, methimazole is generally well-tolerated. However, there have been increasing reports of methimazole-induced liver damage, although this effect of methimazole has been limited by the absence of objective diagnosis of the liver condition or the inappropriate use of the Naranjo scale. We present the case of an elderly man with hyperthyroidism, gastritis, and epilepsy who developed liver damage after administration of multiple drugs. Considering the low sensitivity of the Naranjo scale in detecting rare reactions associated with liver damage, we used the Roussel-Uclaf Causality Assessment Method scale, with a finding of cholestatic jaundice hepatitis induced by methimazole. The patient's liver enzyme levels improved after discontinuation of methimazole. Our case underlines the possible hepatoxicity associated with the use of methimazole. A review of the literature confirmed a selective hepatoxicity risk in individuals of Asian ethnicity, which has not been identified in Caucasian or Black populations. Physicians should be aware of the risk of hepatoxicity when prescribing oral methimazole to patients of Asian ethnicity.

Division B Commission 6: Astronomical Telegrams

Science.gov (United States)

Yamaoka, H.; Green, D. W. E.; Samus, N. N.; Aksnes, K.; Gilmore, A. C.; Nakano, S.; Sphar, T.; Tichá, J.; Williams, G. V.

2016-04-01

IAU Commission 6 ``Astronomical Telegrams'' had a single business meeting during Honolulu General Assembly of the IAU. It took place on Tuesday, 11 August 2015. The meeting was attended by Hitoshi Yamaoka (President), Daniel Green (Director of the Central Bureau for Astronomical Telegrams, CBAT, via Skype), Steven Chesley (JPL), Paul Chodas (JPL), Alan Gilmore (Canterbury University), Shinjiro Kouzuma (Chukyo University), Paolo Mazzali (Co-Chair of the Supernova Working Group), Elena Pian (Scuola Normale Superiore di Pisa), Marion Schmitz (chair IAU Working Group Designations + NED), David Tholen (University of Hawaii), Jana Ticha (Klet Observatory), Milos Tichy (Klet Observatory), Giovanni Valsecchi (INAF\\slash Italy), Gareth Williams (Minor Planet Center). Apologies: Nikolai Samus (General Catalogue of Variable Stars, GCVS).

The history of atopic dermatitis.

Science.gov (United States)

Kramer, Owen N; Strom, Mark A; Ladizinski, Barry; Lio, Peter A

Fred Wise (1881-1950) and Marion Sulzberger (1895-1983) are often credited with introducing the term atopic dermatitis to dermatology in 1933. This definition was based on atopy, a term first created by Arthur Coca (1875-1959) and Robert Cooke (1880-1960) in 1923, when they recognized an association between allergic rhinitis and asthma. Despite its recent introduction into our medical lexicon, historical precursors of atopic dermatitis date back to at least as early as 69-140 ce. In this contribution, we highlight both the prominent individuals credited with shaping the disorder into our current interpretation and the suspected historical precursors of this disease and reported treatments. Copyright © 2017 Elsevier Inc. All rights reserved.

Curcumin inhibits growth potential by G1 cell cycle arrest and induces apoptosis in p53-mutated COLO 320DM human colon adenocarcinoma cells.

Science.gov (United States)

Dasiram, Jade Dhananjay; Ganesan, Ramamoorthi; Kannan, Janani; Kotteeswaran, Venkatesan; Sivalingam, Nageswaran

2017-02-01

Curcumin, a natural polyphenolic compound and it is isolated from the rhizome of Curcuma longa, have been reported to possess anticancer effect against stage I and II colon cancer. However, the effect of curcumin on colon cancer at Dukes' type C metastatic stage III remains still unclear. In the present study, we have investigated the anticancer effects of curcumin on p53 mutated COLO 320DM human colon adenocarcinoma cells derived from Dukes' type C metastatic stage. The cellular viability and proliferation were assessed by trypan blue exclusion assay and MTT assay, respectively. The cytotoxicity effect was examined by lactate dehydrogenase (LDH) cytotoxicity assay. Apoptosis was analyzed by DNA fragmentation analysis, Hoechst and propidium iodide double fluorescent staining and confocal microscopy analysis. Cell cycle distribution was performed by flow cytometry analysis. Here we have observed that curcumin treatment significantly inhibited the cellular viability and proliferation potential of p53 mutated COLO 320DM cells in a dose- and time-dependent manner. In addition, curcumin treatment showed no cytotoxic effects to the COLO 320DM cells. DNA fragmentation analysis, Hoechst and propidium iodide double fluorescent staining and confocal microscopy analysis revealed that curcumin treatment induced apoptosis in COLO 320DM cells. Furthermore, curcumin caused cell cycle arrest at the G1 phase, decreased the cell population in the S phase and induced apoptosis in COLO 320DM colon adenocarcinoma cells. Together, these data suggest that curcumin exerts anticancer effects and induces apoptosis in p53 mutated COLO 320DM human colon adenocarcinoma cells derived from Dukes' type C metastatic stage. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Je-Chun-Jun induced apoptosis of human cervical carcinoma HeLa cells

Institute of Scientific and Technical Information of China (English)

Han-jung CHAE; Kyung-mi PARK; Geun-youn LEE; Gi-seup JEONG; Hyung-rae PARK; Hyung-min KIM; Soo-wan CHAE; Shim-keun YOO; Hyung-ryong KIM

2004-01-01

AIM: To study the mechanism of Je-Chun-Jun (JCJ)-inducing the apoptosis of the human cervical carcinoma,HeLa cells. METHODS: The cell viability was assessed using MTT assay. The optical density was measured at 570 nm. The caspase activity was measured using 50 mmol/L of fluorogenic substrate, AC-DEVD-AMC (caspase3), AC-VEID-AMC (caspase-8) or AC-LEHD-AFC (caspase-9). To confirm the expression of proteins, Western blotting was performed. To detect the characteristic of apoptosis chromatin condensation, HeLa cells were stained with Hoechst 33258 in the presence of JCJ. For the cell cycle analysis, HeLa cells were incubated with Propidium iodide (PI) solution. Fluorescence intensity of cell cycle was measured using flow cytometry system. RESULTS:The loss of viability occurred following the exposure of 10 g/L JCJ. Cells treated with 10 g/L JCJ for 3 d exhibited the apoptotic morphology (brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining) and the reduction of cell volume. Cells incubated with JCJ for 48 h were arrested at the G1 phase of cell cycle and their G1 checkpoint related gene products such as cyclin D1 were transiently decreased. We showed that JCJ induced the p38 MAPK activation in HeLa cells. The p38 MAPK inhibitor, SB203580 protected Hela cells from the JCJ-induced death as well as intervened the JCJ-induced accumulation of cells at the G1 phase. In contrast, MEK1 (-ERK upstream) inhibitor, PD98059 had no effect on HeLa cells. CONCLUSION: JCJ induced cell cycle arrest and apoptosis of HeLa cells through p38 MAPK pathway.

Effects of ethanol on pancreatic beta-cell death: interaction with glucose and fatty acids.

Science.gov (United States)

Dembele, Korami; Nguyen, K Hoa; Hernandez, Tiffany A; Nyomba, B L Grégoire

2009-04-01

Western lifestyle plays an important role in the prevalence of type 2 diabetes by causing insulin resistance and pancreatic beta-cell dysfunction, a prerequisite for the development of diabetes. High fat diet and alcohol are major components of the western diet. The aim of the present study was to investigate the effects of ethanol and fatty acids on beta-cell survival and metabolism. We treated the rat beta-cell line RINm5F with ethanol, a mixture of palmitic and oleic acids, or both. Reactive oxygen species (ROS) were determined by (5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate) (CM-H2DCFDA) fluorescence assay, and mitochondrial activity was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and by determining ATP production. Cell viability was assessed with a cell counter and trypan blue exclusion, and the mode of cell death by Hoechst33342 and propidium iodide staining. With both ethanol and fatty acid treatments, MTT reduction and ATP production decreased, whereas ROS production increased. Ethanol treatment had no effect on cell number, whereas fatty acid treatment reduced the cell number. Cell incubation with ethanol, fatty acids, or both increased the number of Hoechst 33342-positive nuclei. However, the majority of nuclei from fatty acid-treated cells were stained with propidium iodide, indicating a loss of plasma membrane integrity. We conclude that both ethanol and fatty acids generate cellular oxidative stress, and affect mitochondrial function in RINm5F beta-cells. However, ethanol causes beta-cell death by apoptosis, whereas fatty acids cause cell death predominantly by necrosis. It is not known whether these results are applicable to human beta-cells.

Mature Erythrocytes of Iguana iguana (Squamata, Iguanidae Possess Functional Mitochondria.

Directory of Open Access Journals (Sweden)

Giuseppina Di Giacomo

Full Text Available Electron microscopy analyses of Iguana iguana blood preparations revealed the presence of mitochondria within erythrocytes with well-structured cristae. Fluorescence microscopy analyses upon incubation with phalloidin-FITC, Hoechst 33342 and mitochondrial transmembrane potential (Δψm-sensitive probe MitoTracker Red indicated that mitochondria i widely occur in erythrocytes, ii are polarized, and iii seem to be preferentially confined at a "perinuclear" region, as confirmed by electron microscopy. The analysis of NADH-dependent oxygen consumption showed that red blood cells retain the capability to consume oxygen, thereby providing compelling evidence that mitochondria of Iguana erythrocytes are functional and capable to perform oxidative phosphorylation.

Radioimmunoassay of glicagon secretion in diabetes mellitus

International Nuclear Information System (INIS)

Milanova, St.; Koparanova, O.; Milkov, V.; Visheva, N.; Kurtev, I.; Maleeva, A.

1988-01-01

Radioimunoassay of glucagon, lh, fsh, aldosterone, cortisol, acth, sth and lth was performed using preparations from the firms SORIN, AMERSHAM and HOECHST. The basal glucagon secretion was estimated in 403 diabetic patients and 84 normal subjects. It was transitorily suppressed by glucose. The alpha-cellular reactivity in diabetic patients was stimulated by insulin-induced hypoglycemia and with arginine and vasopressin. The tested group of diabetic patients had absolute hyperglucagonemia, despite the hyperglycemia, which is an evidence of abnormal alpha-cellular function. The insulin-dependent nature of hyperglucagonemia in diabetes mellitus and the hyperreactivity of glucagon secretion after protein stimulation was demonstrated. The correlation of these results leads to essential diagnostic inferences

Urban development and employment in Abidjan.

Science.gov (United States)

Joshi, H; Lubell, H; Mouly, J

1975-04-01

The city of Abidjan in the Ivory Coast has grown physically, economically, and demographically at rates exceeding all reasonable expectation. Yet, as in many other development nations, the employment generated by Abidjan's rapid economic expansion has failed to keep pace with the increase in working population it has attracted. Consequently, economic success has been accompanied by a variety of social strains. Some of these have been discussed in earlier issues of the "International Labour Review" by Louis Roussel. This discussion expands on Roussel's earlier treatment by focusing more specifically on several facets of the urban employment problem created by the rapid growth of Abidjan. Attention is directed to labor supply and employment, factors affecting migration, foreign Africans in the Ivory Coast labor force; the urban informal sector; urban infrastructure and development; social problems of population pressure; employment policy options (current government policies and other policy options); and general issues and policy alternatives (motivations for rural urban migration, smaller urban centers as alternative growth poles, and distributing the gains from development). Several essential features of the employment problem stem from the rural urban distribution of the workforce. The rural labor force, including temporary seasonal workers from the savannah countries to the north, remains more or less in balance with increasing rural employment opportunities, since the migration of Ivory Coast nationals to the cities is balanced by the inflow of foreign workers. In contrast, the influx of migrants into urban areas has led to a more rapid increase in the urban labor force than in urban employment, with a consequent rise in unemployment. In 1970 the Abidjan rate of open unemployment was probably around 20%. At this time, most people's idea of a desirable job is one in the formal sector of the urban economy. If there is to be any hope of an eventual balance between

Ergonomie - Sensibilisation à l'ergonomie bureautique - French version only

CERN Multimedia

2003-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et de présentation de bonnes pratiques pour y remédier. Prochaine session en septembre. Les cours sont pris en charge par la division TIS et animés par Clemente Pilly et Ribordy Marion de « PM postures ». L'inscription est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages des formations en sécurité, ou contacter l'organisateur. Organisateur : Ana-Paula Bernardes/TIS-GS (71385) Ana-paula.bernardes@cern.ch

MDMA-induced neurotoxicity of serotonin neurons involves autophagy and rilmenidine is protective against its pathobiology.

Science.gov (United States)

Mercer, Linda D; Higgins, Gavin C; Lau, Chew L; Lawrence, Andrew J; Beart, Philip M

2017-05-01

Toxicity of 3,4-methylenedioxymethamphetamine (MDMA) towards biogenic amine neurons is well documented and in primate brain predominantly affects serotonin (5-HT) neurons. MDMA induces damage of 5-HT axons and nerve fibres and intracytoplasmic inclusions. Whilst its pathobiology involves mitochondrially-mediated oxidative stress, we hypothesised MDMA possessed the capacity to activate autophagy, a proteostatic mechanism for degradation of cellular debris. We established a culture of ventral pons from embryonic murine brain enriched in 5-HT neurons to explore mechanisms of MDMA neurotoxicity and recruitment of autophagy, and evaluated possible neuroprotective actions of the clinically approved agent rilmenidine. MDMA (100 μM-1 mM) reduced cell viability, like rapamycin (RM) and hydrogen peroxide (H 2 O 2 ), in a concentration- and time-dependent manner. Immunocytochemistry revealed dieback of 5-HT arbour: MDMA-induced injury was slower than for RM and H 2 O 2 , neuritic blebbing occurred at 48 and 72 h and Hoechst labelling revealed nuclear fragmentation with 100 μM MDMA. MDMA effected concentration-dependent inhibition of [ 3 H]5-HT uptake with 500 μM MDMA totally blocking transport. Western immunoblotting for microtubule associated protein light chain 3 (LC3) revealed autophagosome formation after treatment with MDMA. Confocal analyses and immunocytochemistry for 5-HT, Hoechst and LC3 confirmed MDMA induced autophagy with abundant LC3-positive puncta within 5-HT neurons. Rilmenidine (1 μM) protected against MDMA-induced injury and image analysis showed full preservation of 5-HT arbours. MDMA had no effect on GABA neurons, indicating specificity of action at 5-HT neurons. MDMA-induced neurotoxicity involves autophagy induction in 5-HT neurons, and rilmenidine via beneficial actions against toxic intracellular events represents a potential treatment for its pathobiology in sustained usage. Copyright © 2017 Elsevier Ltd. All rights reserved.

Transcriptional blockages in a cell-free system by sequence-selective DNA alkylating agents.

Science.gov (United States)

Ferguson, L R; Liu, A P; Denny, W A; Cullinane, C; Talarico, T; Phillips, D R

2000-04-14

There is considerable interest in DNA sequence-selective DNA-binding drugs as potential inhibitors of gene expression. Five compounds with distinctly different base pair specificities were compared in their effects on the formation and elongation of the transcription complex from the lac UV5 promoter in a cell-free system. All were tested at drug levels which killed 90% of cells in a clonogenic survival assay. Cisplatin, a selective alkylator at purine residues, inhibited transcription, decreasing the full-length transcript, and causing blockage at a number of GG or AG sequences, making it probable that intrastrand crosslinks are the blocking lesions. A cyclopropylindoline known to be an A-specific alkylator also inhibited transcription, with blocks at adenines. The aniline mustard chlorambucil, that targets primarily G but also A sequences, was also effective in blocking the formation of full-length transcripts. It produced transcription blocks either at, or one base prior to, AA or GG sequences, suggesting that intrastrand crosslinks could again be involved. The non-alkylating DNA minor groove binder Hoechst 33342 (a bisbenzimidazole) blocked formation of the full-length transcript, but without creating specific blockage sites. A bisbenzimidazole-linked aniline mustard analogue was a more effective transcription inhibitor than either chlorambucil or Hoechst 33342, with different blockage sites occurring immediately as compared with 2 h after incubation. The blockages were either immediately prior to AA or GG residues, or four to five base pairs prior to such sites, a pattern not predicted from in vitro DNA-binding studies. Minor groove DNA-binding ligands are of particular interest as inhibitors of gene expression, since they have the potential ability to bind selectively to long sequences of DNA. The results suggest that the bisbenzimidazole-linked mustard does cause alkylation and transcription blockage at novel DNA sites. in addition to sites characteristic of

Correlation of FMISO simulations with pimonidazole-stained tumor xenografts: A question of O{sub 2} consumption?

Energy Technology Data Exchange (ETDEWEB)

Wack, L. J., E-mail: linda-jacqueline.wack@med.uni-tuebingen.de; Thorwarth, D. [Section for Biomedical Physics, Department of Radiation Oncology, University Hospital Tübingen, Tübingen 72076 (Germany); Mönnich, D. [Section for Biomedical Physics, Department of Radiation Oncology, University Hospital Tübingen, Tübingen 72076 (Germany); German Cancer Consortium (DKTK), Tübingen 72076 (Germany); German Cancer Research Center (DKFZ), Heidelberg 69121 (Germany); Yaromina, A. [OncoRay—National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden 01309, Germany and Department of Radiation Oncology (MAASTRO), GROW—School for Oncology and Developmental Biology, Maastricht University Medical Centre, Maastricht 6229 ET (Netherlands); Zips, D. [German Cancer Consortium (DKTK), Tübingen 72076 (Germany); German Cancer Research Center (DKFZ), Heidelberg 69121 (Germany); Department of Radiation Oncology, University Hospital Tübingen, Tübingen 72076 (Germany); and others

2016-07-15

Purpose: To compare a dedicated simulation model for hypoxia PET against tumor microsections stained for different parameters of the tumor microenvironment. The model can readily be adapted to a variety of conditions, such as different human head and neck squamous cell carcinoma (HNSCC) xenograft tumors. Methods: Nine different HNSCC tumor models were transplanted subcutaneously into nude mice. Tumors were excised and immunoflourescently labeled with pimonidazole, Hoechst 33342, and CD31, providing information on hypoxia, perfusion, and vessel distribution, respectively. Hoechst and CD31 images were used to generate maps of perfused blood vessels on which tissue oxygenation and the accumulation of the hypoxia tracer FMISO were mathematically simulated. The model includes a Michaelis–Menten relation to describe the oxygen consumption inside tissue. The maximum oxygen consumption rate M{sub 0} was chosen as the parameter for a tumor-specific optimization as it strongly influences tracer distribution. M{sub 0} was optimized on each tumor slice to reach optimum correlations between FMISO concentration 4 h postinjection and pimonidazole staining intensity. Results: After optimization, high pixel-based correlations up to R{sup 2} = 0.85 were found for individual tissue sections. Experimental pimonidazole images and FMISO simulations showed good visual agreement, confirming the validity of the approach. Median correlations per tumor model varied significantly (p < 0.05), with R{sup 2} ranging from 0.20 to 0.54. The optimum maximum oxygen consumption rate M{sub 0} differed significantly (p < 0.05) between tumor models, ranging from 2.4 to 5.2 mm Hg/s. Conclusions: It is feasible to simulate FMISO distributions that match the pimonidazole retention patterns observed in vivo. Good agreement was obtained for multiple tumor models by optimizing the oxygen consumption rate, M{sub 0}, whose optimum value differed significantly between tumor models.

Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment.

Science.gov (United States)

Chen, Zongjing; Yang, Yunxiu; Liu, Biao; Wang, Benquan; Sun, Meng; Zhang, Ling; Chen, Bicheng; You, Heyi; Zhou, Mengtao

2015-01-01

Histone deacetylase inhibitors represent a promising class of potential anticancer agents for the treatment of human malignancies. In this study, the effects of trichostatin A (TSA) on apoptosis, metastasis-associated gene expression, and activation of the Notch pathway in human pancreatic cancer cell lines were investigated. After treatment with TSA, cell viability and apoptosis were evaluated using the MTT [3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide] assay, Hoechst 33258 staining, and flow cytometry. Moreover, RT-PCR and western blot analyses were performed to measure the expression levels of apoptosis-associated genes (Bcl-2, Bax, and caspase-3), metastasis-associated genes (E-cadherin, vimentin, and matrix metalloproteinases), and Notch pathway activation (Notch intracellular domain, NICD). The levels of matrix metalloproteinase 2 and NICD were also semi-quantified by immunoassay. Following treatment with TSA for 24 h, PANC-1, SW1990, and MIATACA-2 cells exhibited cell death. The MTT assay revealed that TSA significantly decreased cell viability in a dose-dependent manner in PANC-1 cells. The Hoechst 33258 staining and flow cytometry results evidenced a significant increase in PANC-1 cell apoptosis following TSA treatment. The expression levels of Bax and caspase-3 were increased significantly, whereas Bcl-2 was down-regulated after TSA treatment. In the PANC-1 cells that survived after TSA treatment, the expression levels of vimentin, E-cadherin, and MMP genes were altered by the promotion of potential metastasis and increased expression of NICD. TSA can induce apoptosis of pancreatic cancer cells. In addition, the up-regulation of metastasis-related genes and the activation of the Notch pathway in the survived PANC-1 cells may be associated with a too-low level of TSA or resistance to TSA.

Resveratrol Suppresses Growth and Migration of Myelodysplastic Cells by Inhibiting the Expression of Elevated Cyclin D1 (CCND1).

Science.gov (United States)

Zhou, Wei; Xu, Shilin; Ying, Yi; Zhou, Ruiqing; Chen, Xiaowei

2017-11-01

Myelodysplastic syndromes (MDS) are a group of heterogeneous diseases characterized by poorly formed blood cells. We wanted to elucidate the underlying molecular mechanism to better determine pathogenesis, prognosis, diagnosis, and treatment for patients with MDS. We compared gene expression levels between normal and MDS tissue samples by immunohistochemical analysis. We studied the proliferation, survival, and migration of MDS cells using the EDU assay, colony formation, and transwell assays. We assessed the apoptotic rate and cell cycle status using flow cytometry and Hoechst staining. Finally, we evaluated RNA and protein expressions using polymerase chain reaction and Western blots, respectively. We found that resveratrol suppressed SKM-1 (an advanced MDS cell line) proliferation in a dose-dependent manner. Consistent with this finding, the EDU and colony formation assays also showed that resveratrol inhibited SKM-1 growth. Moreover, flow cytometry and Hoechst 33258 staining demonstrated that resveratrol induced apoptosis and a change in cell cycle status in SKM-1 cells, while the transwell assay showed that resveratrol reduced the migratory ability of SKM-1 cells. Resveratrol also decreased the expression of CCND1 (a gene that encodes the cyclin D1 protein) and increased expressions of KMT2A [lysine (K)-specific methyltransferase 2A] and caspase-3, suggesting that resveratrol exerts its effect by regulating CCND1 in SKM-1 cells. In addition, a combination of resveratrol and the PI3K/AKT inhibitor LY294002 exhibited a stronger inhibitory effect on the SKM-1 cells, compared with resveratrol alone. Our study proved that resveratrol suppresses SKM-1 growth and migration by inhibiting CCND1 expression. This finding provides novel insights into the pathogenesis of MDS and might help develop new diagnosis and treatment for patients with MDS.

[The characters and specific features of new human embryonic stem cells lines].

Science.gov (United States)

Krylova, T A; Kol'tsova, A M; Zenin, V V; Gordeeva, O F; Musorina, A S; Goriachaia, T S; Shlykova, S A; Kamenetskaia, Iu K; Pinaev, G P; Polianskaia, G G

2009-01-01

Four continuous human embryonic stem cell lines (SC1, SC2, SC3 and SC4), derived from the blastocysts has been described. The cell lines were cultivated on mitotically inactivated human feeder cells. The cell lines SC1 and SC2 have passed through 150 population doublings and the cell lines SC3 and SC4 -- near 120 populations doublings, which exceeds Hayflick limit sufficiently. These cell lines maintain high activity of alkaline phosphatase, expression of transcription factor OCT-4 and cell surface antigens (SSEA-4, TRA-1-60 and TRA-1-81), confirming their ESC status and human specificity. Immunofluorescent detection of antigens, characteristic of ectoderm, endoderm and mesoderm confirms the ability of these cells to retain their pluripotency under in vitro condition. PCR analysis revealed expression of six genes specific for pluripotent cells (OCT-4, NANOG, DPPA3/STELLA, TDGF/CRIPTO and LEFTYA). Correlation between the level of proliferative activity and the character of DNA-bound fluorescent staining was found. Fluorescent dyes, Hoechst 33342 and PI, produced diffuse staining of the nuclei in slowly proliferating cells of the SC1 and SC2 lines. In contrast, in actively proliferating cells of the SC3 and SC4 lines, the clear staining of the nuclei was observed. Upon changing the cultivation condition, proliferative activity of SC3 and SC4 lines decreased and became similar to that of SC1 and SC2 lines. The character of the fluorescent staining of all these lines was also shown to be similar. These results show that quality of the fluorescent staining with Hoechst 33342 and PI reflects the level of proliferation. Possible causes and mechanisms of this feature of human ESC are discussed.

Extracting Fluorescent Reporter Time Courses of Cell Lineages from High-Throughput Microscopy at Low Temporal Resolution

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Downey, Mike J.; Jeziorska, Danuta M.; Ott, Sascha; Tamai, T. Katherine; Koentges, Georgy; Vance, Keith W.; Bretschneider, Till

2011-01-01

The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the expression of fluorescent reporters through cell divisions. The ability to track individual cell lineages is essential for the analysis of gene regulatory factors involved in the control of cell fate and identity decisions. In our approach, cell nuclei are identified using Hoechst, and a characteristic drop in Hoechst fluorescence helps to detect dividing cells. We first compare the efficiency and accuracy of different segmentation methods and then present a statistical scoring algorithm for cell tracking, which draws on the combination of various features, such as nuclear intensity, area or shape, and importantly, dynamic changes thereof. Principal component analysis is used to determine the most significant features, and a global parameter search is performed to determine the weighting of individual features. Our algorithm has been optimized to cope with large cell movements, and we were able to semi-automatically extract cell trajectories across three cell generations. Based on the MTrackJ plugin for ImageJ, we have developed tools to efficiently validate tracks and manually correct them by connecting broken trajectories and reassigning falsely connected cell positions. A gold standard consisting of two time-series with 15,000 validated positions will be released as a valuable resource for benchmarking. We demonstrate how our method can be applied to analyze fluorescence distributions generated from mouse stem cells transfected with reporter constructs containing transcriptional control elements of the Msx1 gene, a regulator of pluripotency, in mother and daughter cells. Furthermore, we show by tracking zebrafish PAC2 cells expressing FUCCI cell cycle markers, our framework can be easily adapted to different cell

Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution.

Directory of Open Access Journals (Sweden)

Mike J Downey

Full Text Available The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the expression of fluorescent reporters through cell divisions. The ability to track individual cell lineages is essential for the analysis of gene regulatory factors involved in the control of cell fate and identity decisions. In our approach, cell nuclei are identified using Hoechst, and a characteristic drop in Hoechst fluorescence helps to detect dividing cells. We first compare the efficiency and accuracy of different segmentation methods and then present a statistical scoring algorithm for cell tracking, which draws on the combination of various features, such as nuclear intensity, area or shape, and importantly, dynamic changes thereof. Principal component analysis is used to determine the most significant features, and a global parameter search is performed to determine the weighting of individual features. Our algorithm has been optimized to cope with large cell movements, and we were able to semi-automatically extract cell trajectories across three cell generations. Based on the MTrackJ plugin for ImageJ, we have developed tools to efficiently validate tracks and manually correct them by connecting broken trajectories and reassigning falsely connected cell positions. A gold standard consisting of two time-series with 15,000 validated positions will be released as a valuable resource for benchmarking. We demonstrate how our method can be applied to analyze fluorescence distributions generated from mouse stem cells transfected with reporter constructs containing transcriptional control elements of the Msx1 gene, a regulator of pluripotency, in mother and daughter cells. Furthermore, we show by tracking zebrafish PAC2 cells expressing FUCCI cell cycle markers, our framework can be easily adapted

MDM2 Antagonist Nutlin-3a Reverses Mitoxantrone Resistance by Inhibiting Breast Cancer Resistance Protein Mediated Drug Transport

Science.gov (United States)

Zhang, Fan; Throm, Stacy L.; Murley, Laura L.; Miller, Laura A.; Zatechka, D. Steven; Guy, R. Kiplin; Kennedy, Rachel; Stewart, Clinton F.

2011-01-01

Breast cancer resistance protein (BCRP; ABCG2), a clinical marker for identifying the side population (SP) cancer stem cell subgroup, affects intestinal absorption, brain penetration, hepatobiliary excretion, and multidrug resistance of many anti-cancer drugs. Nutlin-3a is currently under pre-clinical investigation in a variety of solid tumor and leukemia models as a p53 reactivation agent, and has been recently demonstrated to also have p53 independent actions in cancer cells. In the present study, we first report that nutlin-3a can inhibit the efflux function of BCRP. We observed that although the nutlin-3a IC50 did not differ between BCRP over-expressing and vector control cells, nutlin-3a treatment significantly potentiated the cells to treatment with the BCRP substrate mitoxantrone. Combination index calculations suggested synergism between nutlin-3a and mitoxantrone in cell lines over-expressing BCRP. Upon further investigation, it was confirmed that nutlin-3a increased the intracellular accumulation of BCRP substrates such as mitoxantrone and Hoechst 33342 in cells expressing functional BCRP without altering the expression level or localization of BCRP. Interestingly, nutlin-3b, considered virtually “inactive” in disrupting the MDM2/p53 interaction, reversed Hoechst 33342 efflux with the same potency as nutlin-3a. Intracellular accumulation and bi-directional transport studies using MDCKII cells suggested that nutlin-3a is not a substrate of BCRP. Additionally, an ATPase assay using Sf9 insect cell membranes over-expressing wild-type BCRP indicated that nutlin-3a inhibits BCRP ATPase activity in a dose-dependent fashion. In conclusion, our studies demonstrate that nutlin-3a inhibits BCRP efflux function, which consequently reverses BCRP-related drug resistance. PMID:21459080

基于3D细胞培养模型的新型纳米载药系统抗胆囊癌活性的效果及作用机制

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JIANG Han

2014-03-01

Full Text Available ObjectiveTo establish a three-dimensional (3D cell culture model for gallbladder carcinoma (GC cells and to investigate the killing effect of a novel nano-drug delivery system on GC cells and its action mechanism. MethodsTwo-dimensional (2D and 3D cell culture models for GC GBC-SD cells were established, and the normal cell toxicity of nano-drug delivery system (CP24, folate receptor-mediated targeting poly(epsilon-caprolactone and the inhabitation rate of GC cells exposed to 5-fluorouracil (5-FU carried by CP24 were determined by MTT assay, HE staining, flow cytometry (FCM, and Hoechst/PI staining. ResultsCP24 showed no significant cytotoxicity in liver and kidney cells, according to the result of HE staining. CP24 showed no significant killing effect on GC cells, according to the result of FCM. Given a 5-FU concentration of 100 μg/ml, the killing and inhibitory effects of both 5-FU-CP24 and 5-FU on GBC-SD cells were lower when using the 3D cell culture model than when using the 2D cell culture model; whether using a 2D or 3D cell culture model, 5-FU-CP24 had a significantly stronger killing effect on GBC-SD cells than 5-FU (P<0.05. 5-FU-CP24 mainly led to apoptosis in GBC-SD cells, while 5-FU mainly led to necrosis, as shown by Hoechst/PI staining. Conclusion3D cell culture can reflect the intracellular microenvironment in vivo more accurately. Intercellular matrix may affect the cellular sensitivity to drugs. 5-FU can kill more GC cells when carried by a nano-drug delivery system. This degradable nano-drug delivery system has good drug delivery performance, sustained drug release, and high biosafety and holds promise for application in chemotherapy for GC.

Driverless vehicles; Automatisches Fahren

Energy Technology Data Exchange (ETDEWEB)

Goetting, H.H. [MSEE, Goetting KG, Lehrte (Germany)

2000-07-01

New sensor systems, radiocommunication and computer technology have speeded up progress in automation. Driverless vehicles are now feasible. They offer maximum mobility and driving comfort, arrival on time and with minimum environmental effects. Germany should make use of its technological advantage and start trial projects on public roads now. [German] Mit der neuen Sensor-, Funk- und Rechnertechnik ist Bewegung in die Automatisierung gekommen. Automatisches Fahren hat grosse Vorteile fuer unser Wirtschafts- und Sozialleben. Es bietet fuer uns alle ein hoechstes Mass an Mobilitaet, entspannter Bewegung, puenktlicher Ankunft, geringster Belastung fuer die Umwelt (weniger Kraftstoff, weniger Laerm, weniger Platzbedarf). Es ist jetzt entscheidend, dass wir unseren technologischen Vorsprung in Deutschland nutzen, und die ersten Erprobungsprojekte auf oeffentlichen Strassen starten. (orig.)

[Inhibition of gap junctional intercellular communication protects astrocytes from hypoxia/reoxygenation injury].

Science.gov (United States)

Tong, Xu-Hui; Gu, Yu-Chen; Jiao, Hao; Yu, Li; Dong, Shu-Ying

2015-01-01

To investigate the effects of inhibiting gap junctional intercellular communication on hypoxia/reoxygenation injury in astrocytes. Primary cultured cerebral cortical astrocytes of neonate rats were divided into normal control group, hypoxia reoxygenation injury group and 18-α-glycyrrhetinic acid and oleamide (gap junctional intercellular channel inhibitors) group. The gap junction intercellular communication was determined by Parachute assay. The viability of astrocyes was detected by MTT assay. The apoptosis of astrocytes were detected with annexin V/PI and Hoechst 33258 staining. Compared with the normal control group, the gap junctional function of astrocytes was increased significantly in ischemia/reperfusion group (Pastrocytes decreased significantly (Pastrocytes in18-α-glycyrrhetinic acid and oleamide group decreased significantly (Pastrocytes increased significantly (Pastrocytes.

A CONCEPÇÃO ARGUMENTATIVA DE LINGUAGEM NO ENSINO DE REDAÇÃO DE TEXTOS DISSERTATIVO-ARGUMENTATIVOS

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Telisa Furlanetto Graeff

2016-01-01

Full Text Available A crescente demanda no uso de redações de textos dissertativo-argumentativos como instrumento de seleção em concursos para ingresso no ensino superior ou em recrutamento de pessoal para o mercado de trabalho exerce uma pressão, ainda maior, sobre a escola no sentido de didatizar esse gênero textual/discursivo. O presente trabalho propõe uma aplicação da semântica argumentativa, criada em 1983 por Oswald Ducrot e Jean-Claude Anscombre, denominada Argumentation dans la langue (ADL, ao desenvolvimento da competência argumentativa e retórica, especificamente materializada na forma de textos dissertativo-argumentativos escritos. Mais especificamente serão utilizados princípios e conceitos da Teoria dos Blocos Semânticos, versão técnica mais recente da ADL, introduzida pelos estudos de Marion Carel (1992 e desenvolvida juntamente com Ducrot. Palavras-chave: Argumentação linguística. Argumentação retórica. Ensino de dissertação. Abstract The growing demand for the use of writing expository argumentative texts as selection tools on admittance examinations to higher education institutions or when selecting personnel for the working market has put even greater pressure upon schools so as to make such textual/discursive genre didactic. This work proposes a semantic argumentative application, created in 1983 by Oswald Ducrot and Jean-Claude Anscombre, called Argumentation dans la langue (ADL, to the development of argumentative and rhetorical competence, specifically materialised as written expository argumentative texts. More specifically, principles and concepts from the Theory of Semantic Blocks, a newer technical version of LDA, introduced by Marion Carel (1992 and developed with Ducrot, shall be used. Keywords: Linguistic argumentation. Rhetorical argumentation. Teaching dissertation.

Ergonomie - Sensibilisation à l'ergonomie bureautique - French version only

CERN Multimedia

2003-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et de présentation de bonnes pratiques pour y remédier. Prochaine session en juillet et en septembre. Les cours en français, sont pris en charge par la division TIS et animés par Clemente Pilly et Ribordy Marion de « PM postures ». L'inscription est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages des formations en sécurité, ou contacter l'organisateur. Organisateur : Ana-Paula Bernardes/TIS-GS (71385) Ana-paula.bernardes@cern.ch

Medical pluralism: global perspectives on equity issues.

Science.gov (United States)

Marian, Florica

2007-12-01

Over the last decades, awareness has increased about the phenomenon of medical pluralism and the importance to integrate biomedicine and other forms of health care. The broad variety of healing cultures existing alongside biomedicine is called complementary or alternative medicine (CAM) in industrialized countries and traditional medicine (TM) in developing countries. Considerable debate has arisen about ethical problems related to the growing use of CAM in industrialized countries. This article focuses on equity issues and aims to consider them from a global perspective of medical pluralism. Several dimensions of equity are explored and their interrelatedness discussed: access to care, research (paradigm and founding) and recognition. This so-called 'equity circle' is then related to Iris Marion Young's justice theory and particularly to the concepts of cultural imperialism, powerlessness and marginalisation.

Financing Child Care. A Public Policy Report from the Ewing Marion Kauffman Foundation. Winter 2002.

Science.gov (United States)

Ewing Marion Kauffman Foundation, Kansas City, MO.

This public policy report focuses on financing child care in the United States. The report contains brief articles on the following topics: (1) child care wages in comparison to other positions; (2) benefits to businesses when employees have high-quality child care; (3) resources for funding early education systems; (4) comparison of the cost of…

Longleaf Pine Ground-Layer Vegetation in Francis Marion National Forest: Reintroduction, Restoration, and Vegetation Assembly

Energy Technology Data Exchange (ETDEWEB)

Glitzenstein, J.; Streng, D.; Wade, D.

2001-01-01

Study represents significant progress in understanding of compositional gradients in longleaf pine plant communities of Central South Carolina. Study shows the importance of water table depths as a controlling variable with vegetation patterns in the field and similar effects in a garden experiment. Grass planting study suggests that observed field distributions of dormant pine savannah grasses derive from complex interactive effects of fire history, hydrology and light environments. Use of regional longleaf data set to identify candidate species for introduction also appears to be a pioneering effort.

Longleaf Pine Ground-Layer Vegetation in Francis Marion National Forest: Reintroduction, Restoration, and Vegetation Assembly

International Nuclear Information System (INIS)

Glitzenstein, J.; Streng, D.; Wade, D.

2001-01-01

Study represents significant progress in understanding of compositional gradients in longleaf pine plant communities of Central South Carolina. Study shows the importance of water table depths as a controlling variable with vegetation patterns in the field and similar effects in a garden experiment. Grass planting study suggests that observed field distributions of dormant pine savannah grasses derive from complex interactive effects of fire history, hydrology and light environments. Use of regional longleaf data set to identify candidate species for introduction also appears to be a pioneering effort

Pulmonary exposure to carbonaceous nanomaterials and sperm quality

DEFF Research Database (Denmark)

Skovmand, Astrid; Lauvas, Anna Jacobsen; Christensen, Preben

2018-01-01

. Pulmonary inflammation was determined by differential cell count in bronchoalveolar lavage fluid. Epididymal sperm concentration and motility were measured by computer-assisted sperm analysis. Epididymal sperm viability and morphological abnormalities were assessed manually using Hoechst 33,342/PI...... inflammation is a potential modulator of endocrine function. The aim of this study was to investigate the effects of pulmonary exposure to carbonaceous nanomaterials on sperm quality parameters in an experimental mouse model.Methods: Effects on sperm quality after pulmonary inflammation induced by carbonaceous...... flourescent and Spermac staining, respectively. Epididymal sperm were assessed with regard to sperm DNA integrity (damage). Daily sperm production was measured in the testis, and testosterone levels were measured in blood plasma by ELISA.Results: Neutrophil numbers in the bronchoalveolar fluid showed...

Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells

Institute of Scientific and Technical Information of China (English)

Li-Xin Zhu; Jing Liu; You-Hua Xie; Yu-Ying Kong; Ye Ye; Chun-Lin Wang; Guang-Di Li; Yuan Wang

2004-01-01

AIM: To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis.METHODS: A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CHO)cell line. Cell proliferation was assessed by 3H thymidine uptake. Apoptosis was examined by Hoechst 33258staining, flow cytometry and DNA fragmentation analysis.RESULTS: Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage,chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line.CONCLUSION: HCV E2 can induce apoptosis in cultured mammalian cells.

PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN

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Takdir Saili

2009-12-01

Full Text Available Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.

In this tale of Arthur the women do shine

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Carlos A. Sanz Mingo

2012-12-01

Full Text Available This paper aims at analyzing the role of some Arthurian feminine characters incontemporary Arthurian literature. Whilst their medieval counterparts had a mainly passive role andthey did seldom take part in the action of the text, current Arthurian literature has turned this ideaupside down. The first hints at this change could be observed in a few Victorian poems, but it hasbeen in thetwentieth and twenty-first centuries that this trend has shown more popular, not only intexts written by women, as the popularThe Mists of Avalonby Marion Zimmer Bradley, but also inthose written by male authors, as it is the case of Bernard Cornwell’strilogy “The WarlordChronicles”, on which this papers focuses. The study deals with the four most important femininecharacters of the trilogy and how they interact in the political and religious upheavals of the time.

A transformação dos fatos em discurso: um olhar argumentativo = La transformation des faits en discours: un regard argumentatif

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Dall’ Cortivo, Cristiane

2016-01-01

Full Text Available Este artigo realiza um estudo polifônico e argumentativo de dois discursos publicados em dois portais online de notícias. Nosso objetivo é discutir o modo como os locutores constroem sentidos distintos em cada um dos textos a partir do mesmo fato: a divulgação, pela Organização das Nações Unidas, do relatório contendo os dados do Índice de Desenvolvimento Humano. Para embasar este estudo, buscamos o referencial teórico da Teoria da Argumentação na Língua em sua fase atual, a Teoria dos Blocos Semânticos, desenvolvida por Marion Carel e Oswald Ducrot. Nossa hipótese é de que a subjetividade é responsável pela construção de pontos de vista diferentes a respeito da mesma realidade

Climate Change and the Concept of Shared Responsibility

DEFF Research Database (Denmark)

Martinsen, Franziska; Seibt, Johanna

2013-01-01

the spatial to the temporal dimension of such wide-scope results of individual actions. This shift from ‘global ethics’ to ‘intergenerational ethics’ and, in particular, ‘climate ethics’ requires some new analytical concepts, however. In this paper we provide a definition of wide-scope responsibility geared...... to articulate our moral concerns about emergent effects in complex systems, such as climate change. Working from Iris Marion Young’s “social connection model of responsibility”, we present a notion of shared ecological responsibility with global and intergenerational scope. We show that our account...... is not affected by the so-called non-identity objection to intergenerational ethics. Since we work from an action-theoretic rather than normative perspective, our account is ‘ethically parametrized’ in the sense that it can be combined with different conceptions of structural and intergenerational justice...

From essentialism to another way of doing politics: resuming the transformative potential of the politics of difference

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Léa Tosold

2010-12-01

Full Text Available This article aims at resuming and critically scrutinizing the debate on the politics of difference taken among liberal feminist political theorists. Firstly, I shall provide an overview of theoretical attempts to legitimate the politicization of collective-based subjects taking into account the problem of essentialism. Then I will argue that an exclusive focus on the relationship between essentialism and the conceptualization of collective-based subjects tends to overshadow the relationship between the politicization of differences and a new way of doing politics. I will defend the view that the politicization of differences is transforming rather than undermining the functioning of the polity and the possibility of real social justice. I shall evaluate the works of Anne Phillips and Iris Marion Young as offering new ways of reshaping the political sphere in order to enable proper institutional action against structural inequalities.

Feministiske bidrag til politisk teori

DEFF Research Database (Denmark)

Siim, Birte

political theories are based upon a democratic paradox: they are based on assumptions about political equality that contrasts with the political practice where women and minorities are marginalised in relation to political representation, influence and political power. The feminist theories have different...... understandings of politics and power and different visions of how to include women and marginalized groups in democracy. One example is Carole Pateman's theory about the sexual contract that interprets the social contract as an expression of men’s patriarchal power over women rooted in the contradiction between...... the public and private arena. Another contribution is Anne Phillips theory of a "politics of presence" to include both women and marginalized social groups in political institutions by changing the principles of representation, and a third contribution is Iris Marion Young’s "the politics of difference...

Metabolic production of amphetamine following administration of clobenzorex.

Science.gov (United States)

Valtier, S; Cody, J T

1999-01-01

Many of the anorectic drugs that are metabolized to amphetamine and/or methamphetamine pose significant concerns in the interpretation of amphetamine-positive drug testing results. One of these drugs--clobenzorex--has been shown to produce amphetamine. Thirty milligrams of clobenzorex hydrochloride, in the form of a single Asenlix capsule (Roussel, Mexico), were administered orally to five human volunteers with no history of amphetamine, methamphetamine or clobenzorex use. Following administration, urine samples (total void volume) were collected ad lib for seven days and pH, specific gravity and creatinine values were determined. To determine the excretion profile of amphetamine and parent drug, samples were extracted, derivatized, and analyzed by gas chromatography/mass spectrometry (GC/MS) using a standard amphetamine procedure with additional monitoring of ions at m/z 91, 118, 125 and 364 for the detection of clobenzorex. Peak concentrations of amphetamine were detected at 4 to 19 h postdose and ranged from approximately 715 to 2474 ng/mL amphetamine. Amphetamine could be detected (> 5 ng/mL) in the urine in one subject for up to 116 h postdose. GC/MS was also used to determine the enantiomeric composition of the metabolite, amphetamine. This analysis revealed the metabolically derived amphetamine was only the d-enantiomer. This differs from previous literature which indicates clobenzorex is the racemic N-orthochlorobenzyl derivative of amphetamine.

Automatic Slide-Loader Fluorescence Microscope for Discriminative Enumeration of Subseafloor Life

Directory of Open Access Journals (Sweden)

Fumio Inagaki

2010-04-01

Full Text Available The marine subsurface environment is considered the potentially largest ecosystem on Earth, harboring one-tenth of all living biota (Whitman et al., 1998 and comprising diverse microbial components (Inagaki et al., 2003, 2006; Teske, 2006; Inagaki and Nakagawa, 2008. In deep marine sediments, the discrimination of life is significantly more difficult than in surface sediments and terrestrial soils because buried cells generally have extremely low metabolic activities (D’Hondt et al., 2002, 2004, and a highly consolidated sediment matrix produces auto-fluorescence fromdiatomaceous spicules and other mineral particles (Kallmeyer et al., 2008. The cell abundance in marine subsurface sediments has conventionally been evaluated by acridine orange direct count (AODC; Cragg et al., 1995; Parkes et al., 2000 down to 1613 meters below the seafloor (mbsf (Roussel et al., 2008. Since the cell-derived AOsignals often fade out in a short exposure time, recognizing and counting cells require special training. Hence, such efforts to enumerate AO-stained cells from the subseafloor on photographic images have been difficult, and a verification of counts by other methods has been impossible. In addition, providing mean statistical values from low biomass sedimentary habitats has been complicated byphysical and time limitations, yet these habitats are considered critical for understanding the Earth’s biosphere close to the limits of habitable zones (Hoehler, 2004; D’Hondt et al., 2007.

Protective effect of guggulsterone against cardiomyocyte injury induced by doxorubicin in vitro

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Wang Wen-Ching

2012-08-01

Full Text Available Abstract Background Doxorubicin (DOX is an effective antineoplastic drug; however, clinical use of DOX is limited by its dose-dependent cardiotoxicity. It is well known that reactive oxygen species (ROS play a vital role in the pathological process of DOX-induced cardiotoxicity. For this study, we evaluated the protective effects of guggulsterone (GS, a steroid obtained from myrrh, to determine its preliminary mechanisms in defending against DOX-induced cytotoxicity in H9C2 cells. Methods In this study, we used a 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyl-2H-tetrazolium bromide (MTT assay, lactate dehydrogenase (LDH release measurements, and Hoechst 33258 staining to evaluate the protective effect of GS against DOX-induced cytotoxicity in H9C2 cells. In addition, we observed the immunofluorescence of intracellular ROS and measured lipid peroxidation, caspase-3 activity, and apoptosis-related proteins by using Western blotting. Results The MTT assay and LDH release showed that treatment using GS (1–30 μM did not cause cytotoxicity. Furthermore, GS inhibited DOX (1 μM-induced cytotoxicity in a concentration-dependent manner. Hoechst 33258 staining showed that GS significantly reduced DOX-induced apoptosis and cell death. Using GS at a dose of 10–30 μM significantly reduced intracellular ROS and the formation of MDA in the supernatant of DOX-treated H9C2 cells and suppressed caspase-3 activity to reference levels. In immunoblot analysis, pretreatment using GS significantly reversed DOX-induced decrease of PARP, caspase-3 and bcl-2, and increase of bax, cytochrome C release, cleaved-PARP and cleaved-caspase-3. In addition, the properties of DOX-induced cancer cell (DLD-1 cells death did not interfere when combined GS and DOX. Conclusion These data provide considerable evidence that GS could serve as a novel cardioprotective agent against DOX-induced cardiotoxicity.

Chemoresistance in prostate cancer cells is regulated by miRNAs and Hedgehog pathway.

Directory of Open Access Journals (Sweden)

Saurabh Singh

Full Text Available Many prostate cancers relapse due to the generation of chemoresistance rendering first-line treatment drugs like paclitaxel (PTX ineffective. The present study aims to determine the role of miRNAs and Hedgehog (Hh pathway in chemoresistant prostate cancer and to evaluate the combination therapy using Hh inhibitor cyclopamine (CYA. Studies were conducted on PTX resistant DU145-TXR and PC3-TXR cell lines and clinical prostate tissues. Drug sensitivity and apoptosis assays showed significantly improved cytotoxicity with combination of PTX and CYA. To distinguish the presence of cancer stem cell like side populations (SP, Hoechst 33342 flow cytometry method was used. PTX resistant DU145 and PC3 cells, as well as human prostate cancer tissue possess a distinct SP fraction. Nearly 75% of the SP cells are in the G0/G1 phase compared to 62% for non-SP cells and have higher expression of stem cell markers as well. SP cell fraction was increased following PTX monotherapy and treatment with CYA or CYA plus PTX effectively reduced their numbers suggesting the effectiveness of combination therapy. SP fraction cells were allowed to differentiate and reanalyzed by Hoechst staining and gene expression analysis. Post differentiation, SP cells constitute 15.8% of total viable cells which decreases to 0.6% on treatment with CYA. The expression levels of P-gp efflux protein were also significantly decreased on treatment with PTX and CYA combination. MicroRNA profiling of DU145-TXR and PC3-TXR cells and prostate cancer tissue from the patients showed decreased expression of tumor suppressor miRNAs such as miR34a and miR200c. Treatment with PTX and CYA combination restored the expression of miR200c and 34a, confirming their role in modulating chemoresistance. We have shown that supplementing mitotic stabilizer drugs such as PTX with Hh-inhibitor CYA can reverse PTX chemoresistance and eliminate SP fraction in androgen independent, metastatic prostate cancer cell

Exposure to α-Tocopherol, Lutein or Ascorbic Acid improve Cumulus Expansion, Viability and Maturation of Swine Oocytes

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Ileana Miclea

2010-05-01

Full Text Available Protection of the fatty acid and lipid components of oocytes that render them susceptible to free radical or other oxidative injury may prevent the damage currently associated with culture. The goal of this study was to establish the influence of several α-tocopherol, lutein and ascorbic acid concentrations on swine oocyte maturation, viability and the function of cumulus cells in order to improve culture media. Pig oocytes were cultured for 45 hours at 37°C in 5% CO2 atmosphere; in M199 containing several α-tocopherol (5, 10, 20, 40, 80 μM, lutein (2.5, 4, 5, 8, 10 M or ascorbic acid (50, 150, 250, 500, 750 μM concentrations and cumulus expansion was assessed. Afterwards oocytes were coloured using FDA, PI and Hoechst 33258. The differences between treatments were analyzed by the analysis of variance and interpreted using the Newman-Keuls method. When cultured in α-tocopherol supplemented medium the number of expanded COCs to be scored as 3 was significantly greater (p<0.05 for the 5 and 40 μM concentrations. The addition of 8 M lutein to the maturation medium lead to a significant (p<0.05 increase in the number of COCs that were scored at 4. For both α-tocopherol and lutein additions the numbers of oocytes stained by FDA, as well as those stained by Hoechst were greater than the control without being statistically significant. When cultured in 150 and 500 μM ascorbic acid the percentages of COCs scored at 4 were significantly lower (p<0.05 than the control. Also, significantly (p<0.05 fewer oocytes were stained with FDA when matured in 500 μM. Differences between the control and the several concentrations were significant (p<0.05 for 150 and 750 μM and distinctly significant (p<0.01 for 250 μM.

Pretreatment of MQA, a caffeoylquinic acid derivative compound, protects against H2O2-induced oxidative stress in SH-SY5Y cells.

Science.gov (United States)

Tian, Xing; Gao, Lingyue; An, Li; Jiang, Xiaowen; Bai, Junpeng; Huang, Jian; Meng, Weihong; Zhao, Qingchun

2016-12-01

Compound MQA (1,5-O-dicaffeoyl-3-O-[4-malic acid methyl ester]-quinic acid) is a natural caffeoylquinic acid derivative isolated from Arctium lappa L. roots. This study aims to explore the neuroprotective effects of MQA against hydrogen peroxide (H 2 O 2 )-induced oxidative stress in SH-SY5Y neuroblastoma cells. The SH-SY5Y cells were divided into four groups, including control, 20 μM MQA, 200 μM H2O2, 200 μM H2O2 + 20 μM MQA groups. The effects of MQA on H 2 O 2 -induced cell death were measured by MTT and LDH assays. Hoechst 33342 and Annexin V-PI double staining were used to observed H2O2-induced apoptosis. Also, the effects of MQA on antioxidant system and mitochondrial pathway were explored. Further, steady-state phosphorylation levels of ERK1/2, Akt and GSK-3β were examined by Western blot analysis. Pretreatment with MQA prevented cell death in SH-SY5Y cells exposed to 200 μM H2O2 for 3 h. Meanwhile, Hoechst 33342 and Annexin V-PI double staining showed that MQA attenuated H 2 O 2 -induced apoptosis. These changes are related to elevation in SOD activity, reduction in MDA production and ROS formation, and increases in mitochondrial membrane potential (MMP). In addition, the potential mechanisms of MQA against H 2 O 2 -induced apoptosis are associated with increases in the Bcl-2/Bax ratio, decreases in cytochrome c release, caspase-3 and caspase-9 expressions, phosphorylation of ERK1/2, and dephosphorylation of AKT and GSK-3β. These findings suggest that protective effects of MQA against H 2 O 2 -induced apoptosis might be associated with mitochondrial apoptosis, ERK1/2 and AKT/GSK-3β pathway.

Radioprotection of mouse CNS endothelial cells in vivo

International Nuclear Information System (INIS)

Lyubimova, N.; Coultas, P.; Martin, R.

1996-01-01

Full text: Radioprotection using the minor groove binding DNA ligand Hoechst 33342 has been demonstrated in vitro, and more recently in vivo, in mouse lung. Intravenous administration was used for the lung studies, and both endothelial and alveolar epithelial cells-showed good up-take. Radiation damage to the endothelial cell population has also been postulated as important in late developing radionecrosis of spinal cord and brain. Endothelial cell density in brain can be readily determined by a fluorescent-histochemical technique. Treatment with a monoamine oxidase inhibitor and subsequent injection with L-DOPA results in an accumulation of dopamine (DA) in CNS endothelial cells. DA is converted to a fluorophore by exposure to paraformaldehyde, and cell numbers assayed by fluorescence microscopy. Earlier studies used this technique to monitor post-irradiation changes in endothelial cell density in rodent brain and showed the loss, within 24 hours, of a sensitive subpopulation comprising about 15% of the endothelial cells. Ten minutes after intravenous injection of Hoechst 33342 (80mg/kg) the ligand is confined by its limited penetration to the endothelial cells in mouse brain. When we irradiated at this time, there was protection against early endothelial cell loss. Ablation of the sensitive subpopulation in unprotected mice takes place over a dose range of 1 to 3 Gy γ-rays, but doses between 12 to 20 Gy are required in the presence of ligand. This protection equates to a very high dose modification factor of about 7 and possibly reflects a suppression of apoptosis in the sensitive endothelial subpopulation. The extent to which there is enhanced survival in the endothelial population as a whole and how the observed protection affects late CNS necrosis development has yet to be determined. However present results clearly show potential for the use of DNA-binding radioprotectors with limited penetration for investigations into the relative significance of

THESEUS - achieving maximum possible road transport tanker safety by means of experimental accident simulation; THESEUS - Tankfahrzeuge mit hoechsterreichbarer Sicherheit durch experimentelle Unfallsimulation

Energy Technology Data Exchange (ETDEWEB)

Rompe, K.; Heuser, G.

1996-03-01

In spring 1990, the Federal German Minister for Education, Science, Research and Technology (BMBF) commissioned the team from the Federal Institute for Materials Research and Testing (BAM), DEKRA, Daimler-Benz, the Federal Road Research Institute (BASt) with the University of Cologne, Ellinghaus and TUeV Rheinland (project leader) to perform the research project `THESEUS` (the acronym THESEUS comes from the German `Tankfahrzeuge mit hoechst erreichbarer Sicherheit durch experimentelle Unfallsimulation`, which translates as `achieving maximum possible road transport tanker safety by means of experimental accident simulation`). Arting from an analysis of road transport tanker accidents, crash tests and overturn tests were performed and supplemented by investigations of road transport tanker components and accompanying complex calculations of the failure processes. Parallel to this, the static side-tilt stability of road transport tankers was determined on a tilting test platform. The causes of and constructive possibilities for avoiding overturning, which is the most common cause of accidents in which hazardous materials escape, wewre analysed in dynamic driving tests with supplementary computer simulation. Various measures for improving the safety of road transport tankers were determined and subjected to a cost-benefit analysis. (orig.) [Deutsch] Das Bundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie hat seit 1990 das Forschungsproject `THESEUS` (Tankfahrzeuge mit hoechst erreichbarer Sicherheit durch experimentelle Unfallsimulation) der Arbeitsgemeinschaft aus TUeV Rheinland (Federfuehrung), Bundesanstalt fuer Materialforschung und -pruefung (BAM), DEKRA, Daimler Benz, Bundesanstalt fuer Strassenwesen (BASt), Universitaet Koeln und Ellinghaus gefoerdert. Ausgehend von der detaillierten Analyse von 231 Tankfahrzeugunfaellen wurden Tankfahrzeug-Crashversuche und Tankfahrzeug-Umsturzversuche durchgefuehrt. Diese Messungen an kompletten Fahrzeugen

Apoptosis inducing activity of benzophenanthridine-type alkaloids and 2-arylbenzofuran neolignans in HCT116 colon carcinoma cells.

Science.gov (United States)

Mansoor, Tayyab A; Borralho, Pedro M; Luo, Xuan; Mulhovo, Silva; Rodrigues, Cecília M P; Ferreira, Maria-José U

2013-07-15

Thirteen compounds belonging to different classes of alkaloids (1-9) and lignans (10-13), isolated from the methanol extract of roots of the African medicinal plant Zanthoxylum capense, were assayed for their ability as apoptosis inducers in HCT116 colon carcinoma cells. The cytotoxicity of these compounds was evaluated in HCT116 colon carcinoma cells by the MTS assay. Out of the tested compounds, three benzophenanthridine alkaloids (1, 4, and 7), a dibenzyl butyrolactone lignan (10), and two 2-arylbenzofuran neolignans (12 and 13) displayed significant cytotoxicity to HCT116 cells, confirmed by the Guava ViaCount viability assay. The selected compounds (1, 4, 7, 10, 12, and 13) were further tested for apoptosis induction activity in HCT116 cells, by evaluation of nuclear morphology following Hoechst staining, and by caspase-3 like activity assays. Morphologic evaluation of HCT116 nuclei following Hoechst staining and fluorescence microscopy revealed that compounds 1, 4, 7, 10, 12, and 13 induced apoptosis in HCT116 colon carcinoma cells, producing similar, or higher, apoptosis levels when compared with 5-fluorouracil (5-FU), the cornerstone cytotoxic used in colon cancer treatment for several decades. In fact, HCT116 cells developed morphological changes characteristic of apoptosis, including chromatin condensation, nuclear fragmentation and formation of apoptotic bodies. Importantly, compounds 4 and 13 at 20 μM were the most promising in this study, inducing respectively ∼11- and 7-fold increases in apoptotic cells as compared to vehicle control, whereas 5-FU increased apoptosis by ∼2-fold. Apoptosis induction for compounds 4 and 13 was further confirmed by caspase-3-like activity assays, which showed respectively ∼2- and 1.5-fold increases in caspase-3-like activity compared to vehicle control. These results suggested that specific benzophenanthridine alkaloids and 2-arylbenzofuran neolignans isolated from Zanthoxylum capense show strong anticancer

Non-cultured adipose-derived CD45(-) side population cells are enriched for progenitors that give rise to myofibres in vivo

DEFF Research Database (Denmark)

Andersen, Ditte C; Schrøder, Henrik D; Jensen, Charlotte H

2008-01-01

Side population (SP) cells are highly able to exclude the Hoechst 33342 dye through membrane transporters, a feature associated with cell immaturity and therefore proposed as a marker of stem cells. Herein we demonstrate that the adipose tissue derived stromal vascular fraction (SVF) contains...... skeletal muscle repair mainly relies on the satellitecell, several reports have shown that vessel-associated cells may adopt a myogenic phenotype when exposed to a muscle environment. In accordance with these findings, we also observed invitro myogenic specification of SPCD45(-) cells when cocultured...... a novel population of non-haematopoietic "side population" (SPCD45(-)) cells. Simultaneous qRT-PCR of 64 genes revealed that the freshly isolated SPCD45(-) was highly enriched for cells expressing genes related to stem cells, the Notch pathway, and early vascular precursors. Notably, the expression...

Discovery of novel 4-anilinoquinazoline derivatives as potent inhibitors of epidermal growth factor receptor with antitumor activity.

Science.gov (United States)

Xu, Yun-Yun; Li, Si-Ning; Yu, Gao-Jian; Hu, Qing-Hua; Li, Huan-Qiu

2013-10-01

Two new series of new compounds containing a 6-amino-substituted group or 6-acrylamide-substituted group linked to a 4-anilinoquinazoline nucleus have been discovered as potential EGFR inhibitors. These compounds proved efficient effects on antiproliferative activity and EGFR-TK inhibitory activity. Especially, N(6)-((5-bromothiophen-2-yl)methyl)-N(4)-(3-chlorophenyl)quinazoline-4,6-diamine (5e), showed the most potent inhibitory activity (IC50=3.11μM for Hep G2, IC50=0.82μM for A549). The EGFR molecular docking model suggested that the new compound is nicely bound to the region of EGFR, and cell morphology by Hoechst stain experiment suggested that these compounds efficiently induced apoptosis of A549 cells. Copyright © 2013 Elsevier Ltd. All rights reserved.

The effect of hydrostatic pressure on staurosporine-induced neural differentiation in mouse bone marrow‑derived mesenchymal stem cells.

Science.gov (United States)

Javanmard, F; Azadbakht, M; Pourmoradi, M

2016-01-01

In this study, the role of hydrostatic pressure on staurosporine-induced neural differentiation in mouse bone marrow mesenchymal stem cells were investigated. The cells were cultured in treatment medium containing 100 nM of staurosporine for 4 hours; then the cells were affected by hydrostatic pressure (0, 25,50, 100 mmHg). The percentage of cell viability by trypan blue staining and the percentage of cell death by Hoechst/PI differential staining were assessed. We obtained the total neurite length. Expression of β-tubulin III and GFAP (Glial fibrillary acidic protein) proteins were also analyzed by immunocytochemistry. The percentage of cell viability in treatments decreased relative to the increase in hydrostatic pressure and time (p Keywords: bone marrow mesenchymal stem cell, hydrostatic pressure, immunocytochemistry, neural differentiation, neurite length, cell differentiation.

Public Policies of Solar Energy

International Nuclear Information System (INIS)

Bouvier, Yves; Pehlivanian, Sophie; Teissier, Pierre; Chauvin-Michel, Marion; Forget, Marie; Raymond, Roland; Hyun Jin Yu, Julie; Popiolek, Nathalie; Guthleben, Denis

2013-01-01

This dossier about the Public Policies of Solar Energy brings together the presentations given in June 2013 at a colloquium organised by the Savoie university of Chambery (France): Introduction (Yves Bouvier, Sophie Pehlivanian); Passive solar energy in the shade of the French energy policy, 1945-1986 (Pierre Teissier); Solar architectures and energy policies in France: from oil crisis to solar crisis (Marion Chauvin-Michel); Sun in media, between promotion and contestation (Sophie Pehlivanian); Public policies of solar energy and territorial jurisdictions: the example of village photovoltaic power plants (Marie Forget); Energy social system and ordinary creative movement (Roland Raymond); The Historical Evolution of South Korea's Solar PV Policies since the 1970's (Julie Hyun Jin Yu, Nathalie Popiolek); Research on solar energy from yesterday to the present day: an historical project (Denis Guthleben); Photovoltaic power: public policies and economical consequences. The French choices in the international context - 1973-2013 (Alain Ricaud)

SEMÂNTICA E ARGUMENTAÇÃO: DIÁLOGO COM OSWALD DUCROT

Directory of Open Access Journals (Sweden)

Moura Heronides Maurílio de Melo

1998-01-01

Full Text Available Em uma entrevista recente o lingüista Oswald Ducrot discute a Teoria dos Topoi, versão mais recente da Teoria da Argumentação na Língua, desenvolvida por ele e colaboradores. A Teoria dos Topoi é apresentada como um modelo alternativo a uma semântica baseada no conceito de condições de verdade. Na tentativa de lançar luz sobre os postulados dessa teoria, o entrevistador discute com Ducrot e Marion Carel tópicos tais como a relação entre o conceito de pressuposição e a Teoria da Argumentação, a relação entre linguagens lógicas e línguas naturais, a definição de sentido lexical no modelo, e por fim, a traduzibilidade de sentidos lexicais não definidos referencialmente.

Outras vozes na argumentação: atualização da polifonia e reformulação da descrição semântico-argumentativa do discurso

Directory of Open Access Journals (Sweden)

Azevedo, Tânia Maris de

2011-01-01

Full Text Available A concepção polifônica do sentido expressa na Teoria da Argumentação na Língua (TAL vem sendo constantemente reelaborada, desde sua formulação original, por Ducrot, em 1980, até a versão mais recente da TAL, a Teoria dos Blocos Semânticos, de Oswald Ducrot e Marion Carel, acompanhando a evolução da própria Teoria na qual se inscreve. Dado esse percurso, pretendo, neste artigo, dar início à reformulação do modelo por mim proposto para a descrição semântica do discurso pela TAL, especificamente aqui, no que diz respeito ao papel nele exercido pela polifonia, uma vez que o referido modelo toma por base a concepção polifônica da TAL desenvolvida até 2002

Magical arts: the poetics of play.

Science.gov (United States)

Jacobus, Mary

2005-01-01

The paper argues that links between play and magic in British Object Relations point to the persistence of aesthetic concerns within psychoanalysis. Magical thinking is present in British Object Relations psychoanalysis from its beginnings in Klein's play technique and early aesthetic writings, surfacing elsewhere in Susan Isaac's educational experiments and her theories of metaphor. Marion Milner's clinical account of the overlapping areas of illusion and symbol-formation in a boy's war-games link the primitive rituals of Frazer's "The Golden Bough" with her patient's creativity. In Winnicott's concept of the transitional object, the theory of play achieves its apotheosis as a diffusive theory of culture or "private madness," and as a paradigm for psychoanalysis itself. Tracing the non-positivistic, mystical, and poetical elements in British Object Relations underlines the extent to which aesthetics is not just entangled with psychoanalysis, but constitutive of it in its mid-twentieth century manifestations.

Formation en sécurité: Sensibilisation aux gestes et postures de travail - French version only

CERN Multimedia

2004-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. La prochaine session aura lieu le 30 septembre 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Pily Clemente et Marion Ribordy de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d';information et inscription par EDH à ce cours, veuillez consulter les pages Formation et Développement (groupe sécurité) de HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.ch

Ergonomie - Sensibilisation à l'ergonomie bureautique - French version only

CERN Multimedia

2004-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. La prochaine session aura lieu le 30 septembre 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Pily Clemente et Marion Ribordy de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation et Développement (groupe sécurité) des HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.ch

ent-Kaurane Diterpenoids with Neuroprotective Properties from Corn Silk ( Zea mays).

Science.gov (United States)

Qi, Xiao-Li; Zhang, Ying-Ying; Zhao, Peng; Zhou, Le; Wang, Xiao-Bo; Huang, Xiao-Xiao; Lin, Bin; Song, Shao-Jiang

2018-05-25

Thirteen new ent-kaurane diterpenoids, stigmaydenes A-M (1-13), together with two known compounds (14, 15), were isolated from the crude extract of corn silk ( Zea mays). The structures of the compounds were confirmed by comprehensive spectroscopic analyses. The absolute configuration of compound 1 was defined by single-crystal X-ray diffraction. The absolute configurations of the compounds were also confirmed by comparison of experimental and calculated specific rotations. The compounds were evaluated for their neuroprotective effects against H 2 O 2 -induced SH-SY5Y cell injury, and compound 8 was active at 100 μM, as determined by flow cytometry (annexin V-FITC/PI staining) and Hoechst 33258 staining. The results suggested that compound 8 could protect neuronal cells from H 2 O 2 -induced injury by inhibiting apoptosis in SH-SY5Y cells.

Aptamer sensor for cocaine using minor groove binder based energy transfer.

Science.gov (United States)

Zhou, Jinwen; Ellis, Amanda V; Kobus, Hilton; Voelcker, Nicolas H

2012-03-16

We report on an optical aptamer sensor for cocaine detection. The cocaine sensitive fluorescein isothiocyanate (FITC)-labeled aptamer underwent a conformational change from a partial single-stranded DNA with a short hairpin to a double-stranded T-junction in the presence of the target. The DNA minor groove binder Hoechst 33342 selectively bound to the double-stranded T-junction, bringing the dye within the Förster radius of FITC, and therefore initiating minor groove binder based energy transfer (MBET), and reporting on the presence of cocaine. The sensor showed a detection limit of 0.2 μM. The sensor was also implemented on a carboxy-functionalized polydimethylsiloxane (PDMS) surface by covalently immobilizing DNA aptamers. The ability of surface-bound cocaine detection is crucial for the development of microfluidic sensors. Copyright © 2012 Elsevier B.V. All rights reserved.

Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanning microscopy.

Science.gov (United States)

Schulz, Olaf; Pieper, Christoph; Clever, Michaela; Pfaff, Janine; Ruhlandt, Aike; Kehlenbach, Ralph H; Wouters, Fred S; Großhans, Jörg; Bunt, Gertrude; Enderlein, Jörg

2013-12-24

We demonstrate how a conventional confocal spinning-disk (CSD) microscope can be converted into a doubly resolving image scanning microscopy (ISM) system without changing any part of its optical or mechanical elements. Making use of the intrinsic properties of a CSD microscope, we illuminate stroboscopically, generating an array of excitation foci that are moved across the sample by varying the phase between stroboscopic excitation and rotation of the spinning disk. ISM then generates an image with nearly doubled resolution. Using conventional fluorophores, we have imaged single nuclear pore complexes in the nuclear membrane and aggregates of GFP-conjugated Tau protein in three dimensions. Multicolor ISM was shown on cytoskeletal-associated structural proteins and on 3D four-color images including MitoTracker and Hoechst staining. The simple adaptation of conventional CSD equipment allows superresolution investigations of a broad variety of cell biological questions.

Studies on the interaction of the food colorant tartrazine with double stranded deoxyribonucleic acid.

Science.gov (United States)

Basu, Anirban; Suresh Kumar, Gopinatha

2016-05-01

Interaction of the food additive tartrazine with double-stranded DNA was studied by spectroscopic and calorimetric techniques. Absorbance studies revealed that tartrazine exhibited hypochromism in the presence of DNA without any bathochromic effects. Minor groove displacement assay of DAPI and Hoechst 33258 suggested that tartrazine binds in the minor groove of DNA. The complexation was predominantly entropy driven with a smaller but favorable enthalpic contribution to the standard molar Gibbs energy. The equilibrium constant was evaluated to be (3.68 ± .08) × 10(4) M(-1) at 298.15 K. The negative standard molar heat capacity value along with an enthalpy-entropy compensation phenomenon proposed the involvement of dominant hydrophobic forces in the binding process. Tartrazine enhanced the thermal stability of DNA by 7.53 K under saturation conditions.

75 FR 71525 - Prevailing Rate Systems; Redefinition of the Chicago, IL; Fort Wayne-Marion, IN; Indianapolis, IN...

Science.gov (United States)

2010-11-24

... economic impact on a substantial number of small entities because they will affect only Federal agencies... * * * * * PENNSYLVANIA * * * * * PITTSBURGH Survey Area Pennsylvania: Allegheny Beaver Butler Washington Westmoreland...

"Ei maksa seda isatamist väga üle ka pingutada". Lapsevanemate isapuhkusest loobumise diskursused / Marion Pajumets

Index Scriptorium Estoniae

Pajumets, Marion

2007-01-01

Lapsehoolduspuhkusest ning 2008. aasta jaanuaris rakenduvast vanemahüvitise seaduse muudatusest, mis pikendab hüvitise maksmise perioodi nelja kuu võrra ja lubab isadel seda võtta juba laste 70 päevaseks saades. Uurimus isapuhkuse kasutamisest. Tabel

Physiological responses of Pinus sylvestris var. mongolica seedlings to the interaction between Suillus luteus and Trichoderma virens.

Science.gov (United States)

Yin, D; Deng, X; Chet, Ilan; Song, R

2014-09-01

The effects of the interaction between Suillus luteus (L.) Roussel and Trichoderma virens (J.H. Mill., Giddens & A.A. Foster) Arx on Pinus sylvestris var. mongolica Litv. were studied using plant physiology, mycorrhizal science, forest pathology, and biochemistry. Seedling growth and physiological parameters were determined, including the colonization rate of mycorrhizal fungi, biomass, root activity, photosynthetic pigment content, soluble protein content, antioxidant enzyme activities, rhizosphere soil enzyme activities, and protective enzyme activities. In addition, an optimal resistance system involving T. virens, mycorrhizal fungus (S. luteus), and P. sylvestris var. mongolica seedlings was constructed. Synergies between S. luteus and T. virens were observed, and most of the parameters of P. sylvestris var. mongolica seedlings inoculated with S. luteus 30 days + T. virens were higher than other treatments. After three months, when compared the control, the S. luteus 30 days + T. virens treatment gave increases in height (42.3 %); collar diameter (66.7 %); fresh weight (54 %); dry weight (50 %); soluble protein content (69.86 %); root activity (150 %); chlorophyll a (77.6 %); chlorophyll b (70.5 %); carotenoids (144 %); CAT activity (876.9 %); POD activity (268.3 %); SOD activity (66.18 %); β-1,3-glucanase activity (125.8 %); chitinase activity (40 %); rhizosphere soil catalase activity (97.8 %); and phosphatase activity (266.7 %). These results indicate that there may be a stimulating factor between S. luteus and T. virens when they are inoculated together (S. luteus 30 days + T. virens).

Traditional Chinese Medicine and Herb-induced Liver Injury: Comparison with Drug-induced Liver Injury.

Science.gov (United States)

Jing, Jing; Teschke, Rolf

2018-03-28

Cases of suspected herb-induced liver injury (HILI) caused by herbal Traditional Chinese Medicines (TCMs) and of drug-induced liver injury (DILI) are commonly published in the scientific literature worldwide. As opposed to the multiplicity of botanical chemicals in herbal TCM products, which are often mixtures of several herbs, conventional Western drugs contain only a single synthetic chemical. It is therefore of interest to study how HILI by TCM and DILI compare with each other, and to what extent results from each liver injury type can be transferred to the other. China is among the few countries with a large population using synthetic Western drugs as well as herbal TCM. Therefore, China is well suited to studies of liver injury comparing drugs with TCM herbs. Despite some concordance, recent analyses of liver injury cases with verified causality, using the Roussel Uclaf Causality Assessment Method, revealed major differences in HILI caused by TCMs as compared to DILI with respect to the following features: HILI cases are less frequently observed as compared to DILI, have a smaller proportion of females and less unintentional rechallenge events, and present a higher rate of hepatocellular injury features. Since many results were obtained among Chinese residents who had access to and had used Western drugs and TCM herbs, such ethnic homogeneity supports the contention that the observed differences of HILI and DILI in the assessed population are well founded.

Bicalutamide-Associated Acute Liver Injury and Migratory Arthralgia: A Rare but Clinically Important Adverse Effect

Directory of Open Access Journals (Sweden)

Helga M. Gretarsdottir

2018-06-01

Full Text Available We describe a case of acute liver injury and migratory arthralgia in a patient receiving bicalutamide treatment for prostate cancer. A 67-year-old male with metastatic prostate cancer presented with a 6-day history of migratory arthralgia. He had been undergoing treatment with bicalutamide for 4 months; 3 weeks prior to symptom appearance the bicalutamide dose had been increased. He had no other symptoms. Liver tests and inflammatory markers were markedly elevated. Serology for hepatitis viruses A, B, and C, CMV, and EBV and autoimmune causes were all negative, and an ultrasound of the upper abdomen was normal. There was no history of blood transfusion, intravenous drug abuse, or alcohol abuse. Due to the suspicion of a drug-induced symptomatology, bicalutamide was discontinued and the patient started on 30 mg prednisolone daily. Three weeks later he was symptom free and after 6 weeks his liver tests were almost normal. The Roussel Uclaf Causality Assessment Method (RUCAM suggested a high probability of liver injury. Bicalutamide has very rarely been reported as a causative agent for liver injury and to our knowledge never for migratory polyarthralgia. The migratory polyarthralgia was attributed to bicalutamide due to the absence of other etiological factors and the disappearance of symptoms after discontinuation of the drug. To our knowledge, this is the first published case report of migratory arthralgia and concomitant liver injury attributed to bicalutamide.

Ways to prepare future teachers to teach science in multicultural classrooms

Science.gov (United States)

Billingsley, Berry

2016-06-01

Roussel De Carvalho uses the notion of superdiversity to draw attention to some of the pedagogical implications of teaching science in multicultural schools in cosmopolitan cities such as London. De Carvalho makes the case that if superdiverse classrooms exist then Science Initial Teacher Education has a role to play in helping future science teachers to become more knowledgeable and reflective about how to teach school students with a range of worldviews and religious beliefs. The aim of this paper is to take that proposition a step further by considering what the aims and content of a session in teacher education might be. The focus is on helping future teachers develop strategies to teach school students to think critically about the nature of science and what it means to have a scientific worldview. The paper draws on data gathered during an interview study with 28 students at five secondary schools in England. The data was analysed to discover students' perceptions of science and their perceptions of the way that science responds to big questions about being human. The findings are used to inform a set of three strategies that teachers could use to help young people progress in their understanding of the nature of science. These strategies together with the conceptual framework that underpins them are used to develop a perspective on what kinds of pedagogical content knowledge teacher education might usefully provide.

Macromolecular mechanisms of sputum inhibition of tobramycin activity.

Science.gov (United States)

Hunt, B E; Weber, A; Berger, A; Ramsey, B; Smith, A L

1995-01-01

Tobramycin, an aminoglycoside antibiotic, is used in the treatment of Pseudomonas aeruginosa infections in cystic fibrosis patients. Tobramycin bioactivity, however, is antagonized by sputum. Glycoproteins (mucins) and high-molecular-weight DNA make up 2 to 3% (P. L. Masson and J. F. Heremans, p. 412-475, In M. J. Dulfano, ed., Sputum: Fundamentals and Clinical Pathology, 1973) and 3 to 10% (W. S. Chernick and G. J. Barbero, Pediatrics 24:739-745, 1959, and R. Picot, I. Das, and L. Reid, Thorax 33:235-242, 1978) of the dry weight of sputum, respectively. tobramycin binds to both mucins and DNA obtained from sputum (R. Ramphal, M. Lhermitte, M. Filliat, and P. Roussel, J. Antimicrob. Chemother. 22:483-490, 1988). In vitro, recombinant human DNase (rhDNase) hydrolyzes high-molecular-weight DNA of > 50 kb within sputum to fragments of 2 to 4 kb. Studying dialyzable tobramycin, we examined drug binding to whole sputum and to "mock sputum," which consisted of porcine gastric mucin and calf thymus DNA. We also studied the effects of rhDNase treatments of sputum, mock sputum, and calf thymus DNA on tobramycin binding. We found that treatments of sputum, mock sputum, and calf thymus DNA with rhDNase did not significantly increase the tobramycin bioactivity within the dialysates; surprisingly, sputum binding of tobramycin was increased by rhDNase. We conclude that rhDNase does not increase the bioactivity of tobramycin in sputum.

New Onset Autoimmune Hepatitis during Anti-Tumor Necrosis Factor-Alpha Treatment in Children.

Science.gov (United States)

Ricciuto, Amanda; Kamath, Binita M; Walters, Thomas D; Frost, Karen; Carman, Nicholas; Church, Peter C; Ling, Simon C; Griffiths, Anne M

2018-03-01

To evaluate a large anti-tumor necrosis factor (TNF)-treated pediatric inflammatory bowel disease cohort for drug-induced liver injury (DILI) following presentation of an index case with suspected DILI with autoimmune features after infliximab exposure. To characterize the incidence, natural history, and risk factors for liver enzyme elevation with anti-TNF use. We reviewed the index case and performed a retrospective cohort study of 659 children receiving anti-TNF therapy between 2000 and 2015 at a tertiary pediatric inflammatory bowel disease center. Patients with alanine aminotransferase (ALT) ≥×2 the upper limit of normal were included. The incidence, evolution, and risk factors for liver injury were examined with univariate and multivariable proportional hazards regression. Causality was assessed using the Roussel-Uclaf Causality Assessment Method. The index case, a teenage girl with Crohn's disease, developed elevated liver enzymes and features of autoimmune hepatitis on liver biopsy 23 weeks after starting infliximab. The injury resolved entirely within 4 months of withdrawing infliximab without additional therapy. Overall, 7.7% of our cohort developed new ALT elevations while on anti-TNF. Most ALT elevations were mild and transient and attributable to alternate etiologies. No additional clear cases of autoimmune hepatitis were identified. Transient liver enzyme abnormalities are relatively common among anti-TNF-treated children. Anti-TNF-related DILI with autoimmune features is rare but must be recognized so that therapy can be stopped. Copyright © 2017 Elsevier Inc. All rights reserved.

WHY GENDER MAINSTREAMING IS NOT ENOUGH? A CRITIQUE TO SYLVIA WALBY’S THE FUTURE OF FEMINISM

Directory of Open Access Journals (Sweden)

Oana Crușmac

2015-03-01

Full Text Available The present paper represents a detailed analysis of the proposals made by Sylvia Walby in “The Future of Feminism”. The structure of the article follows two main directions, according to Walby’s discourse, respectively feminism outside the state and feminism inside the state/transnational institutions. The first direction will focus on feminist theories and will canvass their present forms and also the postfeminist trends. The second direction will examine feminist projects which have entered into the transnational and state policies – gender mainstreaming, affirmative action, equality of treatment, reconfiguration of the human rights. Another important section will be dedicated to inequalities within the family and how can gender policies correct them and why Walby omits this aspect from her analysis. The nature of gender inequalities shall be presented both from the point of view of distributive theories as well as the standpoint of the theory of oppression as a source of inequality, according to its formulation made by Iris Marion Young

THE VARIATION OF SOME BIOCHEMICAL PARAMETERS FROM FRESH COW CHEESE CREAM TYPE WITH ADDED AROMATIC PLANTS

Directory of Open Access Journals (Sweden)

CRISTIANA DIACONESCU

2007-10-01

Full Text Available Recent advanced studies in the field of cheese fabrication technology include, amongother, the usage of aromatic plants and spices as a mean of improving theorganoleptic properties and as a mean of decreasing the lipidic peroxidationphenomenon. For this purpose, three types of fresh cow cheese produced by S.C.Marion Invest Trade 94 SRL, experimentally added with dill, savory and rosemary,were comparatively studied from a chemical point of view (pH, acidity, humidity, ashand a biochemical point of view(raw fat, total proteins, total cholesterol, acidphosphatase’s enzymatic activity, superoxide dismutase’s enzymatic activity, calciumcontent.From a chemical point of view, no significant differences appeared between the creamcheese types analyzed, excepting the pH( lower in the cheeses with dill and rosemaryFrom a biochemical point of view, two phenomenon can be observed: a significantdecrease of the acidic phosphatase activity in the cheeses with added dill androsemary ( positively correlating with the pH value and decreased values of the SODactivity in all cheese types.

Genealogy of John and Charles Bell: their relationship with the children of Charles Shaw of Ayr.

Science.gov (United States)

Kaufman, M

2005-11-01

The Reverend William Bell had six children who survived infancy. Two of his sons entered the legal profession and two other sons became distinguished anatomists and surgeons--John Bell, said for 20 years to have been the leading operating surgeon in Britain and throughout the world--and Sir Charles Bell, possibly the most distinguished anatomist and physiologist of his day. Information is not known about the fifth son or their sister. Charles Shaw, a lawyer of Ayr, had four sons and two daughters who survived infancy. Two of his sons, John and Alexander, became anatomists and later surgeons at the Middlesex Hospital, and both worked closely with Charles Bell at the Great Windmill Street School of Anatomy. His third son entered the law and his fourth son became a distinguished soldier. The two daughters of Charles Shaw married into the Bell family: Barbara married George Joseph Bell and Marion married Mr (later Sir) Charles Bell.

LIDERAZGO Y CONOCIMIENTO COMPARTIDO EN CONTEXTOS INTERORGANIZACIONALES

Directory of Open Access Journals (Sweden)

GUSTAVO ADOLFO ESGUERRA PÉREZ

2017-01-01

Full Text Available Este documento presenta una descripción teórica, desde la perspectiva relacional, de uno de los factores determinantes de ventaja competitiva: las rutinas de conocimiento compartido (Dyer & Singh, 1998, las cuales se consideran fundamental en el logro de rentas relacionales, y se revi- san algunos elementos dirigidos a comprender su desarrollo. También se describe la función del liderazgo entendido como el ejercicio deliberado de influencia para el logro de objetivos (Huxham & Vangen, 2000, en este caso, compartir conocimiento. Se proponen además, los tipos y carac- terísticas de liderazgo que pueden emerger específicamente en el proceso de transferencia y com- partimiento de conocimiento interorganizacional, desde las propuestas de Cox, Pearce & Sims (2011 y Uhl-Bien & Marion (2008. Para terminar se señalan las implicaciones y perspectivas que de este trabajo se desprenden para la in vestigación en el campo.

Estimates of natural streamflow at two streamgages on the Esopus Creek, New York, water years 1932 to 2012

Science.gov (United States)

Burns, Douglas A.; Gazoorian, Christopher L.

2015-01-01

Streamflow in the Esopus Creek watershed is altered by two major watershed management activities carried out by the New York City Department of Environmental Protection as part of its responsibility to maintain a water supply for New York City: (1) diversion of water from the Schoharie Creek watershed to the Esopus Creek through the Shandaken Tunnel, and (2) impoundment of the Esopus Creek by a dam that forms the Ashokan Reservoir and subsequent release through the Catskill Aqueduct. Stakeholders in the Catskill region are interested and concerned about the extent to which these watershed management activities have altered streamflow, especially low and high flows, in the Esopus Creek. To address these concerns, natural (in the absence of diversion and impoundment) daily discharge from October 1, 1931, to September 30, 2012, was estimated for the U.S. Geological Survey streamgages at Coldbrook (station number 01362500), downstream of the Shandaken Tunnel discharge, and at Mount Marion (01364500), downstream of the Ashokan Reservoir.

Pb-Sr-Nd isotopic data of Indian Ocean ridges: New evidence of large-scale mapping of mantle heterogeneities

International Nuclear Information System (INIS)

Hamelin, B.; Dupre, B.; Allegre, C.J.

1986-01-01

A Pb-Sr-Nd isotope study of South West and East Indian Ridges confirms that the Indian Ocean belongs to a specific regional isotopic domain, as previously suggested by the results from islands of this ocean. The isotopic domain defined by the Indian MORB is indeed different from that of the North Atlantic and East Pacific Oceans. This demonstrates that the convective circulation of the upper mantle does not allow a rapid homogenization from one region to the other. The isotopic data of the Indian ridges can be interpreted by a contamination model, in which the depleted upper mantle (identical to that under the North Atlantic) is contaminated by two different types of contaminant, one corresponding to the source of the ''central Indian Ocean'' islands (Amsterdam, St. Paul, Marion, Prince Edward, Reunion, Rodriguez, Mauritius), and the other to a source similar to that of Walvis or Ninety East aseismic ridges. These two contaminants would have contributed to the ridge volcanism in different proportion over time. (orig.)

Safety Training: Ergonomie - Sensibilisation à l'ergonomie bureautique

CERN Multimedia

2004-01-01

If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. SAFETY TRAINING Laetitia Laddada tel. 73811 - 79236 safety.training@cern.ch Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 11 mars 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Clemente Pilly et Ribordy Marion de « PM postures...

Formation en sécurité : Ergonomie - Sensibilisation à l'ergonomie bureautique

CERN Multimedia

2004-01-01

If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. SAFETY TRAINING Laetitia Laddada tel. 73811 - 79236 safety.training@cern.ch Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 6 mai 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Clemente Pilly et Ribordy Marion de « PM postures&...

Formation en sécurité : Ergonomie - Sensibilisation à l'ergonomie bureautique

CERN Multimedia

2004-01-01

If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. SAFETY TRAINING Laetitia Laddada tel. 73811 - 79236 safety.training@cern.chNous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 6 mai 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Clemente Pilly et Ribordy Marion de « PM postures&...

Safety Training: Ergonomie - Sensibilisation à l'ergonomie bureautique - French version only

CERN Multimedia

Laetitia Laddada

2004-01-01

If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Formation en SECURITE SAFETY Training Laetitia Laddada 73811 - 79236 safety.training@cern.ch Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 8 juillet 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Clemente Pilly et Ribordy Marion de « PM postures &ra...

Ergonomie - Sensibilisation à l'ergonomie bureautique - French version only

CERN Multimedia

2004-01-01

If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. SAFETY TRAINING Laetitia Laddada tel. 73811 - 79236 safety.training@cern.chNous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 12 février et le 11 mars 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Clemente Pilly et Ribordy Marion de &laq...

THE STUDY AND TREATMENT OF MOTHERS AND INFANTS, THEN AND NOW: MELANIE KLEIN'S "NOTES ON BABY" IN A CONTEMPORARY PSYCHOANALYTIC CONTEXT.

Science.gov (United States)

Aguayo, Joseph; Salomonsson, Björn

2017-04-01

This paper draws on Melanie Klein's (unpublished) observational notes of her infant grandson, written primarily in 1938 and 1939. Apart from moving glimpses into a young family's life, the notes contain astute observations of an infant's behavior and emotions. Compared with Klein's published writings, the style is less theoretical and polemical. Later, in his latency years, Klein's grandson was in analysis with Marion Milner, who in 1952 published a paper drawing on the treatment. The present paper focuses on (1) how observations and treatment of the same child and his family by clinicians in close relationships with each other (Klein, Milner, and Winnicott) fertilized reciprocal influence but also brought into question the validity of Klein's observations, and (2) the relative merits and contributions of various modalities in understanding the infant's psyche, including experimental research, direct observation, parent-infant psychotherapy, and reconstructions from older patients-as occurs, for example, in psychoanalysis. © 2017 The Psychoanalytic Quarterly, Inc.

Die ontwikkeling van die menslike bewussyn: Teorieë oor die ontwikkeling van menslike bewussyn – die ontdekking van die ‘misterie van die siel’

Directory of Open Access Journals (Sweden)

Ignatius (Naas W. Ferreira

2009-10-01

A number of theories on the development of human consciousness have tried to incorporate all views on the subject into one integral theory. However, Ken Wilber is the first philosopher who managed to combine the external with the internal fields of study. Using Wilber’s integral theory, a number of researchers developed their own theories in their field of speciality. Jim Marion used Wilber’s development theory to show the evolution of consciousness from a Christian perspective. Steve McIntosh, an integral philosopher, takes Wilber’s ideas further, and even criticises him on a few points. Another important researcher following Wilber is Andre Marquis, who developed an integral questionnaire to help pastors gauge clients’ problems. James Fowler, Clare Graves and Bill Plotkin also researched the evolution of human consciousness. This article examines each of these researchers, and concludes with a glance at several viewpoints on the soul and the mystical union with God.

On the evaluation of global sea-salt aerosol models at coastal/orographic sites

Science.gov (United States)

Spada, M.; Jorba, O.; Pérez García-Pando, C.; Janjic, Z.; Baldasano, J. M.

2015-01-01

Sea-salt aerosol global models are typically evaluated against concentration observations at coastal stations that are unaffected by local surf conditions and thus considered representative of open ocean conditions. Despite recent improvements in sea-salt source functions, studies still show significant model errors in specific regions. Using a multiscale model, we investigated the effect of high model resolution (0.1° × 0.1° vs. 1° × 1.4°) upon sea-salt patterns in four stations from the University of Miami Network: Baring Head, Chatam Island, and Invercargill in New Zealand, and Marion Island in the sub-antarctic Indian Ocean. Normalized biases improved from +63.7% to +3.3% and correlation increased from 0.52 to 0.84. The representation of sea/land interfaces, mesoscale circulations, and precipitation with the higher resolution model played a major role in the simulation of annual concentration trends. Our results recommend caution when comparing or constraining global models using surface concentration observations from coastal stations.

Safety Training: Ergonomie - Sensibilisation à l'ergonomie bureautique

CERN Multimedia

Laetitia Laddada

2005-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 24 février 2005 (matin et après-midi). Les cours sont pris en charge par la Commission de Sécurité et animés par Pily Clemente et Marion Ribordy de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation et Développement (groupe sécurité) de HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.ch FORMATION EN SECURITE SAFETY TRAINING Laetitia Laddada ...

Safety Training: Ergonomie - Sensibilisation à l'ergonomie bureautique

CERN Multimedia

Laetitia Laddada

2005-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 24 février 2005 (matin et après-midi). Les cours sont pris en charge par la Commission de Sécurité et animés par Pily Clemente et Marion Ribordy de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation et Développement (groupe sécurité) de HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.ch FORMATION EN SECURITE SAFETY TRAINING Laetitia Laddada 7...

Safety Training: Ergonomie - Sensibilisation à l'ergonomie bureautique

CERN Multimedia

2005-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 24 février 2005 (matin et après-midi). Les cours sont pris en charge par la Commission de Sécurité et animés par Pily Clemente et Marion Ribordy de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation et Développement (groupe sécurité) de HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.ch FORMATION EN SECURITE SAFETY TRAINING safety.training@ce...

Safety Training: Ergonomie - Sensibilisation à l'ergonomie bureautique

CERN Multimedia

Laetitia Laddada

2004-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. La prochaine session aura lieu le 30 septembre 2004 (matin). Les cours sont pris en charge par la Commission de Sécurité et animés par Pily Clemente et Marion Ribordy de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation & Développement (Groupe Sécurité) des HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.ch FORMATION EN SECURITE SAFETY TRAINING Laetitia Laddada 73811 - 79236 ...

Safety Training: Ergonomie - Sensibilisation à l'ergonomie bureautique

CERN Multimedia

Laetitia Laddada

2004-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. La prochaine session aura lieu le 30 septembre 2004 (matin). Les cours sont pris en charge par la Commission de Sécurité et animés par Pily Clemente et Marion Ribordy de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation & Développement (Groupe Sécurité) des HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.ch FORMATION EN SECURITE SAFETY TRAINING Laetitia Laddada 73811 - 79236 ...

ERGONOMIE - SENSIBILISATION A L'ERGONOMIE BUREAUTIQUE

CERN Multimedia

Laetitia Laddada

2004-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 6 mai 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Clemente Pilly et Ribordy Marion de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation et Développement (groupe sécurité) de HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) / Ana-paula.bernardes@cern.ch FORMATION EN SECURITE Laetitia Laddada Tél. 73811 - 79236 safety.training@cern.ch

Safety Training: Ergonomie - sensibilisation à l'ergonomie bureautique - French version only

CERN Multimedia

Laetitia Laddada

2004-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 8 juillet 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Clemente Pilly et Ribordy Marion de « PM postures ». L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation et Développement (groupe sécurité) de HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.ch Formation en SECURITE SAFETY Training Laetitia Laddada 73811 - 79236 safety.training@cern.ch

Safety training: Ergonomie - Sensibilisation à l'ergonomie bureautique

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Laetitia Laddada

2004-01-01

Nous vous proposons une 1/2 journée de sensibilisation sur les risques engendrés par une mauvaise posture lors du travail sur écran (mal de dos, fatigue visuelle, douleurs des poignets...) et des bonnes pratiques pour y remédier. Les prochaines sessions auront lieu le 8 juillet 2004. Les cours sont pris en charge par la Commission de Sécurité et animés par Pily Clemente et Marion Ribordy de « PM postures » . L'inscription via EDH est obligatoire. Pour plus d'information et inscription par EDH à ce cours, veuillez consulter les pages Formation et Développement (groupe sécurité) de HR, ou contacter l'organisateur. Les places seront attribuées dans l'ordre de réception des inscriptions. Organisateur : Ana-Paula Bernardes/SC-GS (71385) Ana-paula.bernardes@cern.chFORMATION EN SECURITE SAFETY TRAINING Laetitia Laddada 73811 - 79236 safety.training@cern.ch

A comparative study on the efficacy of four anthelmintics on some important reindeer parasites

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Magnus Nordkvist

1983-05-01

Full Text Available Four anthelmintic preparations were tested against some of the most important parasites of reindeer, i.e. warble fly (Oedemagena tarandi, nostril fly (Cephenemyia trompe, brainworm (Elaphostrongylus rangiferi, and lungworm (Dictyocaulus viviparus. Their efficacy against intestinal nematodes was also registered. Test drugs were Fenthion (Bayer, Fenbendazole (Hoechst, Mebendazole (Janssen, and Ivermectin (Merk Sharp & Dohme. Against O. tarandi and C. trompe Ivermectin was 100% effective and Fenthion 86 and 100% respectively. The efficacy of Fen- and Mebendazole against these parasites was not significant. Against E. rangiferi the benzimidazole compounds were highly effective, with Mebendazole a bit ahead. Ivermectin had a moderate effect and Fenthion had no effect on this parasite. Against D. viviparus Fenbendazole, Mebendazole and Ivermectin were of equal, moderate-high effectiveness. No drug had a complete effect on the «arrested» larvae of D. viviparus. Fenthion had no effect at all. Fenbendazole and Ivermectin were both 100% effective against intestinal nematodes. Mebendazole was less effective and Fenthion had no effects. Ivermectin is considered to be the overall most effective anthelmintic in this test.En jamforande studie av effekten av fyra anthelmintika mot några betydelsesfulla parasiter hos ren.Abstract in Swedish / Sammandrag: Fyra antiparasitmedel har prôvats mot några av renens viktigaste parasiter, nàmligen hudkorm (Oedemagena tarandi, svalgkorm (Cephenemyia trompe, hjårnmask (Elaphostrongylus rangiferi och lungmask (Dictyocaulus viviparus. Vidare har medlens effekt på mag- tarmnematoder (Trichostongylider också noterats. De prôvade medicinerna var Fenthion (Bayer, Mebendazole (Leo/Janssen, Fenbendazole (Hoechst och Ivermectin (Merck Sharp & Dohme. Mot hud- och svalgkorm var Ivermectin 100% effektivt medan for Fenthion effekten var 86 resp 100%. Effekten av Fen- och Mebendazole mot de båda parasiterna var inte

INGN 201: Ad-p53, Ad5CMV-p53, Adenoviral p53, INGN 101, p53 gene therapy--Introgen, RPR/INGN 201.

Science.gov (United States)

2003-01-01

Introgen's adenoviral p53 gene therapy [INGN 201, ADVEXIN] is in clinical development for the treatment of various cancers. The p53 tumour suppressor gene is deleted or mutated in many tumour cells and is one of the most frequently mutated genes in human tumours. INGN 201 has been shown to kill cancer cells directly. In August 2002, Introgen announced plans to file an application for INGN 201 with the European Agency for the Evaluation of Medicinal Products (EMEA) for the treatment of head and neck cancer; the European filing will be submitted simultaneously with the previously scheduled (planned for 2004) submission of a Biologics License Application (BLA) for ADVEXIN to the US FDA. On 20 February 2003, INGN 201 received orphan drug designation from the US FDA for head and neck cancer. INGN 201 is available for licensing although Introgen favours retaining partial or full rights to the therapy in the US. Introgen Therapeutics and its collaborative partner for the p53 programme, Aventis Gencell, have been developing p53 gene therapy products. The agreement was originally signed by Rhône-Poulenc Rorer's Gencell division, which became Aventis Gencell after Rhône-Poulenc Rorer merged with Hoechst Marion Roussel to form Aventis Pharma. According to the original agreement, Introgen was responsible for phase I and preclinical development in North America, while Aventis Gencell was responsible for clinical trials conducted in Europe and for clinical trials in North America beyond phase I. In April 2001, Aventis Gencell and Introgen restructured their existing collaboration agreement for p53 gene therapy products. Aventis Gencell indicated that p53 research had suffered from internal competition for resources and was pulling back from its development agreement with Introgen for p53 gene therapy products. Introgen will assume responsibility for worldwide development of all p53 programmes and will obtain exclusive worldwide commercial rights to p53-based gene therapy

Polimixina B: efeito dose e tempo dependente na nefrotoxicidade in vitro Polymyxin B: dose and time dependent nephrotoxicity effect in vitro

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Luciana Barros de Moura Neiva

2013-01-01

Full Text Available OBJETIVO: Caracterizar a toxicidade da polimixina B (PmxB em células renais em dosagem e tempos diferentes. MÉTODOS: Células LLC-PK1, cultivadas em placas multiwell de 12 poços, foram divididas nos seguintes grupos: Controle (CTL - células mantidas em meio DMEM suplementado a 5%; G1 - células expostas à concentração de 75mM de PmxB; G2 - células expostas à concentração de 375mM de PmxB. Cada grupo foi avaliado nos tempos de 24, 48 e 72 horas quanto à viabilidade celular (Acridine Orange/Brometo de Etídio e apoptose (Hoechst 33342. RESULTADOS: Os dados demonstraram a viabilidade celular e a apoptose à exposição de três doses de PmxB em três intervalos de tempo, com um aumento significativo da toxicidade à elevação das doses e ao maior tempo de permanência no antibiótico para apoptose. CONCLUSÃO: A citotoxicidade pela PmxB, no modelo de cultivo celular, se mostrou tempo e dose dependente, aumentando com a maior exposição e maior dose de antibiótico.OBJECTIVE: To characterize the toxicity of polymyxin B (PmxB in renal cell in different dosage and times. METHODS: LLC-PK1 cells grown in 12 well multiwell plates were divided into the following groups: Control (CTL - cells maintained in DMEM supplemented with 5%; G1 - cells exposed to concentration of 75µM PmxB G2 - cells exposed to concentration of 375µM PmxB. Each group was assessed at 24,48 and 72 hours as for cell viability (Acridine orange/ethidium bromide and apoptosis (Hoechst 33342. RESULTS: The data demonstrate the cell viability and apoptosis exposure of three doses of PmxB in three time intervals, with a significant increase in toxicity to high doses and longer duration of stay in the antibiotic to apoptosis. CONCLUSION: Cytotoxicity by PmxB in cell culture model, showed to be time and dose dependent, increasing with increased exposure and higher dose of antibiotic.

Albumin-induced apoptosis of glomerular parietal epithelial cells is modulated by extracellular signal-regulated kinase 1/2

Science.gov (United States)

Ohse, Takamoto; Krofft, Ron D.; Wu, Jimmy S.; Eddy, Allison A.; Pippin, Jeffrey W.; Shankland, Stuart J.

2012-01-01

Background. The biological role(s) of glomerular parietal epithelial cells (PECs) is not fully understood in health or disease. Given its location, PECs are constantly exposed to low levels of filtered albumin, which is increased in nephrotic states. We tested the hypothesis that PECs internalize albumin and increased uptake results in apoptosis. Methods. Confocal microscopy of immunofluorescent staining and immunohistochemistry were used to demonstrate albumin internalization in PECs and to quantitate albumin uptake in normal mice and rats as well as experimental models of membranous nephropathy, minimal change disease/focal segmental glomerulosclerosis and protein overload nephropathy. Fluorescence-activated cell sorting analysis was performed on immortalized cultured PECs exposed to fluorescein isothiocyanate (FITC)-labeled albumin in the presence of an endosomal inhibitor or vehicle. Apoptosis was measured by Hoechst staining in cultured PECs exposed to bovine serum albumin. Levels of phosphorylated extracellular signal-regulated kinase 1 and 2 (p-ERK1/2) were restored by retroviral infection of mitogen-activated protein kinase (MEK) 1/2 and reduced by U0126 in PECs exposed to high albumin levels in culture and apoptosis measured by Hoechst staining. Results. PECs internalized albumin normally, and this was markedly increased in all of the experimental disease models (P PECs also internalize FITC-labeled albumin, which was reduced by endosomal inhibition. A consequence of increased albumin internalization was PEC apoptosis in vitro and in vivo. Candidate signaling pathways underlying these events were examined. Data showed markedly reduced levels of phosphorylated extracellular signal-regulated kinase 1 and 2 (ERK1/2) in PECs exposed to high albumin levels in nephropathy and in culture. A role for ERK1/2 in limiting albumin-induced apoptosis was shown by restoring p-ERK1/2 by retroviral infection, which reduced apoptosis in cultured PECs, while a forced

Quantitative imaging of tumor vasculature using multispectral optoacoustic tomography (MSOT)

Science.gov (United States)

Tomaszewski, Michal R.; Quiros-Gonzalez, Isabel; Joseph, James; Bohndiek, Sarah E.

2017-03-01

The ability to evaluate tumor oxygenation in the clinic could indicate prognosis and enable treatment monitoring, since oxygen deficient cancer cells are often more resistant to chemotherapy and radiotherapy. MultiSpectral Optoacoustic Tomography (MSOT) is a hybrid technique combining the high contrast of optical imaging with spatial resolution and penetration depth similar to ultrasound. We hypothesized that MSOT could reveal both tumor vascular density and function based on modulation of blood oxygenation. We performed MSOT on nude mice (n=8) bearing subcutaneous xenograft PC3 tumors using an inVision 256 (iThera Medical). The mice were maintained under inhalation anesthesia during imaging and respired oxygen content was modified from 21% to 100% and back. After imaging, Hoechst 33348 was injected to indicate vascular perfusion and permeability. Tumors were then extracted for histopathological analysis and fluorescence microscopy. The acquired data was analyzed to extract a bulk measurement of blood oxygenation (SO2MSOT) from the whole tumor using different approaches. The tumors were also automatically segmented into 5 regions to investigate the effect of depth on SO2MSOT. Baseline SO2MSOT values at 21% and 100% oxygen breathing showed no relationship with ex vivo measures of vascular density or function, while the change in SO2MSOT showed a strong negative correlation to Hoechst intensity (r=- 0.92, p=0.0016). Tumor voxels responding to oxygen challenge were spatially heterogeneous. We observed a significant drop in SO2 MSOT value with tumor depth following a switch of respiratory gas from air to oxygen (0.323+/-0.017 vs. 0.11+/-0.05, p=0.009 between 0 and 1.5mm depth), but no such effect for air breathing (0.265+/-0.013 vs. 0.19+/-0.04, p=0.14 between 0 and 1.5mm depth). Our results indicate that in subcutaneous prostate tumors, baseline SO2MSOT levels do not correlate to tumor vascular density or function while the magnitude of the response to oxygen

DaonilÒ y glibenclamida 5 mg de producción nacional: liberación in vitro Daonil ® and glibenclamide 5 mg of national production: release in vitro

Directory of Open Access Journals (Sweden)

Diana Pereda Rodríguez

2007-04-01

Full Text Available Dentro del proceso global de la liberación de un fármaco, la disolución es el paso más importante pues está íntimamente relacionado con los procesos de absorción, determinantes en la biodisponibilidad de un medicamento administrado por vía oral. En el presente trabajo se realizaron los perfiles de disolución de 2 lotes de DaonilÒ (Hoechst Farma SA, Barcelona, medicamento líder del principio activo glibenclamida (DCI, y de 3 lotes de glibenclamida 5 mg de producción nacional. Los datos de porcentaje de principio activo liberado contra tiempo se sometieron a un estudio de ajuste a 4 modelos comunes a perfiles de disolución mediante el programa CurveExpert y se realizó la comparación estadística de los perfiles de disolución con la utilización de un método independiente de modelo matemático (f2: factor de similitud. Los lotes estudiados no cumplen con los criterios de la Food and Drug Administration (FDA para los estudios de equivalencia in vitro y muestran diferencias significativas (p 0,05 sus perfiles de disolución.Dissolution is the most important step in the global release process of a drug, since it is closely related to the absorption processes, which are determinant in the bioavailability of a drug administered by oral route. The dissolution profiles of two batches of Daonil® (Hoechst Farma SA Barcelona, a leading drug of the glibenclamide active principle (DCI, and of 3 batches of glibenclamide 5 mg of national production were studied. The percentage data of the active principle released against time were subjected to a study of adjustment of 4 models to common dissolution profiles by the CurveExpert program, and the statistical comparison of dissolution profiles was made by using a mathematical independent model (f2: similarity factor. All the batches studied do not fulfill the criteria of the Food and Drug Administration (FDA for the in vitro equivalence studies and showed significant differences in their

Cellular effect of styrene substituted biscoumarin caused cellular apoptosis and cell cycle arrest in human breast cancer cells.

Science.gov (United States)

Perumalsamy, Haribalan; Sankarapandian, Karuppasamy; Kandaswamy, Narendran; Balusamy, Sri Renukadevi; Periyathambi, Dhaiveegan; Raveendiran, Nanthini

2017-11-01

Coumarins occurs naturally across plant kingdoms exhibits significant pharmacological properties and pharmacokinetic activity. The conventional, therapeutic agents are often associated with poor stability, absorption and increased side effects. Therefore, identification of a drug that has little or no-side effect on humans is consequential. Here, we investigated the antiproliferative activity of styrene substituted biscoumarin against various human breast cancer cell lines, such as MCF-7, (ER-) MDA-MB-231 and (AR+) MDA-MB-453. Styrene substituted biscoumarin induced cell death by apoptosis in MDA-MB-231 cell line was analyzed. Antiproliferative activity of Styrene substituted biscoumarin was performed by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Styrene substituted biscoumarin induced apoptosis was assessed by Hoechst staining, Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) staining and flow cytometric analysis. Migratory and proliferating characteristic of breast cancer cell line MDA-MB-231 was also analyzed by wound healing and colony formation assay. Furthermore, mRNA expression of BAX and BCL-2 were quantified using qRT-PCR and protein expression level analyzed by Western blot. The inhibition concentration (IC 50 ) of styrene substituted biscoumarin was assayed against three breast cancer cell lines. The inhibition concentration (IC 50 ) value of styrene substituted biscoumarin toward MDA-MB-231, MDA-MB-453 and MCF-7 cell lines was 5.63, 7.30 and 10.84μg/ml respectively. Styrene substituted biscoumarin induced apoptosis was detected by Hoechst staining, DAPI/PI analysis and flow-cytometric analysis. The migration and proliferative efficiency of MDA-MB-231 cells were completely arrested upon styrene substituted biscoumarin treatment. Also, mRNA gene expression and protein expression of pro-apoptotic (BAX) and anti-apoptotic (BCL-2) genes were analyzed by qRT-PCR and western blot analysis upon

Real-time Tumor Oxygenation Changes After Single High-dose Radiation Therapy in Orthotopic and Subcutaneous Lung Cancer in Mice: Clinical Implication for Stereotactic Ablative Radiation Therapy Schedule Optimization

Energy Technology Data Exchange (ETDEWEB)

Song, Changhoon [Department of Radiation Oncology, Seoul National University College of Medicine, Seoul (Korea, Republic of); Hong, Beom-Ju; Bok, Seoyeon; Lee, Chan-Ju; Kim, Young-Eun [Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Gyeongbuk (Korea, Republic of); Jeon, Sang-Rok [Department of Radiation Oncology, Seoul National University College of Medicine, Seoul (Korea, Republic of); Cancer Research Institute, Seoul National University College of Medicine, Seoul (Korea, Republic of); Wu, Hong-Gyun [Department of Radiation Oncology, Seoul National University College of Medicine, Seoul (Korea, Republic of); Cancer Research Institute, Seoul National University College of Medicine, Seoul (Korea, Republic of); Institute of Radiation Medicine, Medical Research Center, Seoul National University College of Medicine, Seoul (Korea, Republic of); Lee, Yun-Sang [Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul (Korea, Republic of); Department of Molecular Medicine and Biopharmaceutical Sciences, Seoul National University College of Medicine, Seoul (Korea, Republic of); Cheon, Gi Jeong; Paeng, Jin Chul [Cancer Research Institute, Seoul National University College of Medicine, Seoul (Korea, Republic of); Institute of Radiation Medicine, Medical Research Center, Seoul National University College of Medicine, Seoul (Korea, Republic of); Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul (Korea, Republic of); Carlson, David J. [Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut (United States); and others

2016-07-01

Purpose: To investigate the serial changes of tumor hypoxia in response to single high-dose irradiation by various clinical and preclinical methods to propose an optimal fractionation schedule for stereotactic ablative radiation therapy. Methods and Materials: Syngeneic Lewis lung carcinomas were grown either orthotopically or subcutaneously in C57BL/6 mice and irradiated with a single dose of 15 Gy to mimic stereotactic ablative radiation therapy used in the clinic. Serial [{sup 18}F]-misonidazole (F-MISO) positron emission tomography (PET) imaging, pimonidazole fluorescence-activated cell sorting analyses, hypoxia-responsive element-driven bioluminescence, and Hoechst 33342 perfusion were performed before irradiation (day −1), at 6 hours (day 0), and 2 (day 2) and 6 (day 6) days after irradiation for both subcutaneous and orthotopic lung tumors. For F-MISO, the tumor/brain ratio was analyzed. Results: Hypoxic signals were too low to quantitate for orthotopic tumors using F-MISO PET or hypoxia-responsive element-driven bioluminescence imaging. In subcutaneous tumors, the maximum tumor/brain ratio was 2.87 ± 0.483 at day −1, 1.67 ± 0.116 at day 0, 2.92 ± 0.334 at day 2, and 2.13 ± 0.385 at day 6, indicating that tumor hypoxia was decreased immediately after irradiation and had returned to the pretreatment levels at day 2, followed by a slight decrease by day 6 after radiation. Pimonidazole analysis also revealed similar patterns. Using Hoechst 33342 vascular perfusion dye, CD31, and cleaved caspase 3 co-immunostaining, we found a rapid and transient vascular collapse, which might have resulted in poor intratumor perfusion of F-MISO PET tracer or pimonidazole delivered at day 0, leading to decreased hypoxic signals at day 0 by PET or pimonidazole analyses. Conclusions: We found tumor hypoxia levels decreased immediately after delivery of a single dose of 15 Gy and had returned to the pretreatment levels 2 days after irradiation and had decreased

(3'R)-hydroxytabernaelegantine C: A bisindole alkaloid with potent apoptosis inducing activity in colon (HCT116, SW620) and liver (HepG2) cancer cells.

Science.gov (United States)

Paterna, Angela; Gomes, Sofia E; Borralho, Pedro M; Mulhovo, Silva; Rodrigues, Cecília M P; Ferreira, Maria-José U

2016-12-24

Tabernaemontana elegans Stapf. (Apocynaceae) is a medicinal plant traditionally used in African countries to treat cancer. To discover new apoptosis inducing lead compounds from T. elegans and provide scientific validation of the ethnopharmacological use of this plant. Through fractionation, (3'R)-hydroxytaberanelegantine C (1), a vobasinyl-iboga bisindole alkaloid, was isolated from a cytotoxic alkaloid fraction of the methanol extract of T. elegans roots. Its structure was identified by spectroscopic methods, mainly 1D and 2D NMR experiments. Compound 1 was evaluated for its ability to induce apoptosis in HCT116 and SW620 colon and HepG2 liver carcinoma cells. The cell viability of compound 1 was evaluated by the MTS and lactate dehydrogenase (LDH) assays. Induction of apoptosis was analyzed through Guava ViaCount assay, by flow cytometry, caspase-3/7 activity assays and evaluation of nuclear morphology by Hoechst staining. To determine the molecular pathways elicited by 1 exposure, immunoblot analysis was also performed. (3'R)-hydroxytaberanelegantine C (1) displayed strong apoptosis induction activity as compared to 5-fluorouracil (5-FU), the most used anticancer agent in colorectal cancer treatment. In the MTS assay, compound 1 exhibited IC 50 values similar or lower than 5-FU in the three cell lines tested. The IC 50 value of 1 was also calculated in CCD18co normal human colon fibroblasts. The lactate dehydrogenase assay showed increased LDH release by compound 1, and the Guava ViaCount assay revealed that 1 significantly increased the incidence of apoptosis to a further extent than 5-FU. Moreover, the induction of apoptosis was corroborated by evaluation of nuclear morphology by Hoechst staining and caspase-3/7 activity assays of 1 treated cells. As expected, in immunoblot analysis, compound 1 treatment led to poly(ADP-ribose) polymerase cleavage. This was accompanied by decreased anti-apoptotic proteins Bcl-2 and XIAP steady state levels in all three cancer

Measurement of serum type III procollagen aminopeptide with RIA-gnost PIIIP coated tube

International Nuclear Information System (INIS)

Maruyama, Katsuya; Takagi, Toshikazu; Okazaki, Isao

1992-01-01

In 55 patients with various alcoholic liver diseases, serum type III procollagen aminopeptide (PIIIP) was measured on admission, on biopsy (2-4 weeks after admission) and on discharge from hospital (3 months after admission) using RIA-gnost PIIIP coated tube, an assay kit recently launched by Hoechst Japan Limited. Serum PIIIP on admission showed a good correlation with the degree of hepatic fibrosis and was significantly higher in any disease group than in healthy controls, suggesting its usefulness as an indicator of hepatic fibrosis. After abstinence, however, serum PIIIP level exhibited an increasing tendency in all the disease groups expect alcoholic hepatitis, and the number of laboratory tests showing a significant correlation with PIIIP decreased as compared with that on admission. The mechanism of serum PIIIP elevations seen after abstience is yet to be elucidated in detail, considering their possible association with factors other than collagen synthesis in the liver. (author)