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Sample records for histoplasma capsulatum darling

  1. owing to Histoplasma capsulatum infection

    African Journals Online (AJOL)

    numerous foam cells (Fig. 7). Special staining techniques (periodic acid. Schiff and silver stain) showed the presence of multiple yeast cells both within and outside the foamy histiocytes (Figs 8 and 9). Some yeast cells demonstrated budding. Morphologically, the fungus was consistent with. Histoplasma capsulatum.

  2. Dimorfismo y patogenia de Histoplasma capsulatum Dimorphism and pathogenesis of Histoplasma capsulatum

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    C. E. López

    2006-12-01

    Full Text Available Histoplasma capsulatum es un hongo patógeno dimorfo de importancia en todo el mundo, que causa un amplio espectro de enfermedades. Vive en estado saprobio en fase micelial, presentando hifas con dos tipos de conidios solitarios, macro y microconidias. La infección con H. capsulatum se inicia por vía respiratoria con la inhalación de propágulos fúngicos, constituidos principalmente por microconidios de 1-4 x 2-6 µm o de fragmentos hifales de 5 a 8 µm, que llegan a los bronquiolos terminales y alvéolos pulmonares. Los propágulos inhalados se convierten entonces a la fase levaduriforme, responsable de la patogénesis del H. capsulatum. Por ser un hongo del suelo sin requerimientos conocidos para interactuar con un hospedador mamífero como parte del ciclo de vida obligado, sus estrategias de patogénesis son particularmente notables. Entre éstas se incluyen la transición dimorfa micelio-levadura, entrada en las células fagocíticas del hospedador, localización subcelular, supervivencia y proliferación intracelular durante la infección activa y persistencia durante la infección clínicamente inaparente, con capacidad de reactivación. La patogénesis de H. capsulatum fue estudiada ampliamente a partir del aumento de pacientes inmunosuprimidos. Esta publicación presenta un resumen de los avances realizados en las investigaciones del dimorfismo y la patogénesis de H. capsulatum.Histoplasma capsulatum is a dimorphic fungal pathogen with worldwide significance, which causes a broad spectrum of disease. In the saprophytic stage, it lives as a mycelial form consisting of hyphae bearing both macro and microconidia. Infection with H. capsulatum occurs by inhalation of microconidia (1-4 x 2-6 µm or small mycelia fragments (5-8 µm in the terminal bronchioles and alveoli of the lung. Inhaled conidia then convert into the yeast form that is responsible for the pathogenesis of histoplasmosis. As a soil fungus with no known requirements for

  3. Could Histoplasma capsulatum Be Related to Healthcare-Associated Infections?

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    Laura Elena Carreto-Binaghi

    2015-01-01

    Full Text Available Healthcare-associated infections (HAI are described in diverse settings. The main etiologic agents of HAI are bacteria (85% and fungi (13%. Some factors increase the risk for HAI, particularly the use of medical devices; patients with severe cuts, wounds, and burns; stays in the intensive care unit, surgery, and hospital reconstruction works. Several fungal HAI are caused by Candida spp., usually from an endogenous source; however, cross-transmission via the hands of healthcare workers or contaminated devices can occur. Although other medically important fungi, such as Blastomyces dermatitidis, Paracoccidioides brasiliensis, and Histoplasma capsulatum, have never been considered nosocomial pathogens, there are some factors that point out the pros and cons for this possibility. Among these fungi, H. capsulatum infection has been linked to different medical devices and surgery implants. The filamentous form of H. capsulatum may be present in hospital settings, as this fungus adapts to different types of climates and has great dispersion ability. Although conventional pathogen identification techniques have never identified H. capsulatum in the hospital environment, molecular biology procedures could be useful in this setting. More research on H. capsulatum as a HAI etiologic agent is needed, since it causes a severe and often fatal disease in immunocompromised patients.

  4. Could Histoplasma capsulatum Be Related to Healthcare-Associated Infections?

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    Carreto-Binaghi, Laura Elena; Damasceno, Lisandra Serra; Mendes-Giannini, Maria José Soares; Zancopé-Oliveira, Rosely Maria; Taylor, Maria Lucia

    2015-01-01

    Healthcare-associated infections (HAI) are described in diverse settings. The main etiologic agents of HAI are bacteria (85%) and fungi (13%). Some factors increase the risk for HAI, particularly the use of medical devices; patients with severe cuts, wounds, and burns; stays in the intensive care unit, surgery, and hospital reconstruction works. Several fungal HAI are caused by Candida spp., usually from an endogenous source; however, cross-transmission via the hands of healthcare workers or contaminated devices can occur. Although other medically important fungi, such as Blastomyces dermatitidis, Paracoccidioides brasiliensis, and Histoplasma capsulatum, have never been considered nosocomial pathogens, there are some factors that point out the pros and cons for this possibility. Among these fungi, H. capsulatum infection has been linked to different medical devices and surgery implants. The filamentous form of H. capsulatum may be present in hospital settings, as this fungus adapts to different types of climates and has great dispersion ability. Although conventional pathogen identification techniques have never identified H. capsulatum in the hospital environment, molecular biology procedures could be useful in this setting. More research on H. capsulatum as a HAI etiologic agent is needed, since it causes a severe and often fatal disease in immunocompromised patients. PMID:26106622

  5. Identification of Histoplasma capsulatum from culture extracts by real-time PCR.

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    Martagon-Villamil, Jose; Shrestha, Nabin; Sholtis, Mary; Isada, Carlos M; Hall, Gerri S; Bryne, Terry; Lodge, Barbara A; Reller, L Barth; Procop, Gary W

    2003-03-01

    We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.

  6. Histoplasma capsulatum sinusitis: Possible way of revelation to the disseminated form of histoplasmosis in HIV patients

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    Elansari, R.; Abada, R.; Rouadi, S.; Roubal, M.; Mahtar, M.

    2016-01-01

    Introduction: Histoplasma capsulatum is rarely found in nose and sinuses in immunocompetent and even in immunosupressed patients. A literature review revealed rare cases of H. capsulatum sinusitis and the case we describe in this paper is the first case of H. capsulatum sinusitis in Morocco. The purpose of our work is to present a rare case of H. capsulatum sinusitis and a literature review. Case report: A male patient, at his 39 presented to the emergency with suffered from nasal congesti...

  7. In vitro inhibitory effect of miltefosine against strains of Histoplasma capsulatum var. capsulatum and Sporothrix spp.

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    Brilhante, Raimunda Sâmia Nogueira; Malaquias, Angela Donato Maia; Caetano, Erica Pacheco; Castelo-Branco, Débora de Souza Collares Maia; Lima, Rita Amanda Chaves de; Marques, Francisca Jakelyne de Farias; Silva, Natalya Fechine; Alencar, Lucas Pereira de; Monteiro, André Jalles; Camargo, Zoilo Pires de; Bandeira, Tereza de Jesus Pinheiro Gomes; Rodrigues, Anderson Messias; Cordeiro, Rossana de Aguiar; Moreira, José Luciano Bezerra; Sidrim, José Júlio Costa; Rocha, Marcos Fábio Gadelha

    2014-04-01

    Miltefosine (MIL), originally developed for use in cancer chemotherapy, has been shown to have important antifungal activity against several pathogenic fungi. Our aim in this study was to determine the in vitro activity of MIL against the dimorphic fungi Histoplasma capsulatum and Sporothrix spp. This was done using the broth microdilution method. MIL had an in vitro inhibitory effect against all strains of H. capsulatum var. capsulatum and Sporothrix spp. analyzed. The minimal inhibitory concentrations (MIC) varied from 0.25 μg/ml to 2 μg/ml for H. capsulatum var. capsulatum in the filamentous phase and from 0.125 μg/ml to 1 μg/ml in the yeast phase. The MIC interval for Sporothrix spp. in the filamentous phase was 0.25-2 μg/ml. The minimal fungicidal concentrations (MFCs) were ≤4 μg/ml for isolates of both analyzed species. This study demonstrates that MIL has an antifungal effect in vitro against two potentially pathogenic fungi and that more studies should be performed in order to evaluate its applicability in vivo.

  8. Autonomous replication of foreign DNA in Histoplasma capsulatum: role of native telomeric sequences.

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    Woods, J P; Goldman, W E

    1993-01-01

    Genetic transformation of the dimorphic pathogenic fungus Histoplasma capsulatum can result in chromosomal integration of the transforming DNA or the generation of multicopy linear plasmids carrying the transforming DNA. We showed previously that Escherichia coli plasmids do not replicate autonomously in H. capsulatum without significant modifications, one of which is the in vivo addition of Histoplasma telomeres at the termini of linear DNA. To address the requirements for autonomous replica...

  9. Validation of western blot for Histoplasma capsulatum antibody detection assay.

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    Almeida, Marcos de Abreu; Pizzini, Cláudia Vera; Damasceno, Lisandra Serra; Muniz, Mauro de Medeiros; Almeida-Paes, Rodrigo; Peralta, Regina Helena Saramago; Peralta, José Mauro; Oliveira, Raquel de Vasconcelos Carvalhaes; Vizzoni, Alexandre Gomes; de Andrade, Carla Lourenço Tavares; Zancopé-Oliveira, Rosely Maria

    2016-02-24

    Histoplasmosis is worldwide systemic mycoses caused by the dimorphic fungus Histoplasma capsulatum. The isolation and identification of H. capsulatum in culture is the reference test for histoplasmosis diagnosis confirmation. However, in the absence of it, serology has been used as a presumptive diagnosis through antibody and antigen detection. The purpose of the present study was to validate an immunoassay method (western blot) for antibodies detection in the diagnosis of histoplasmosis. To validate the western blot (WB) a study was conducted using 118 serum samples from patients with histoplasmosis and 118 serum controls collected from January 2000 to December 2013 in residents of the Rio de Janeiro State, Brazil. Diagnostic validation parameters were calculated based on the categorization of results obtained in a 2 × 2 table and subjected to statistical analysis. In addition, the viability of deglycosylated histoplasmin antigen (ptHMIN) onto nitrocellulose membranes previously sensitized was evaluated during the same period. The WB test showed sensitivity of 94.9 %, specificity of 94.1 %, positive predictive value of 94.1 %, negative predictive value of 94.9 %, accuracy of 94.5 %, and almost perfect precision. Besides, the strips have proved to be viable for using at least 5 years after ptHMIN antigen sensitization. Western blot test using ptHMIN provides sensitive, specific, and faster results. Therefore, could be considered a useful tool in the diagnosis of histoplasmosis being used by public health system, even in situations where laboratory facilities are relatively limited.

  10. Glycosylation and Immunoreactivity of the Histoplasma capsulatum Cfp4 Yeast-Phase Exoantigen

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    Holbrook, Eric D.; Kemski, Megan M.; Richer, Sarah M.; Wheat, L. Joseph; Rappleye, Chad A.

    2014-01-01

    The yeast phase of Histoplasma capsulatum is the virulent form of this thermally dimorphic fungal pathogen. Among the secreted proteome of Histoplasma, culture filtrate protein 4 (Cfp4) is a heavily glycosylated factor produced abundantly and specifically by Histoplasma yeast cells, suggesting its role in pathogenesis. We have generated three monoclonal antibodies as tools for characterization and detection of Cfp4 and determined the epitope each recognizes. Through site-directed mutagenesis ...

  11. Molecular characterization of Histoplasma capsulatum isolated from an outbreak in treasure hunters Histoplasma capsulatum in treasure hunters

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    Muñoz Bertha

    2010-09-01

    Full Text Available Abstract Background In Mexico, primary pulmonary histoplasmosis is the most relevant clinical form of the disease. The geographical distribution of specific strains of Histoplasma capsulatum circulating in Mexico has not been fully established. Outbreaks must be reported in order to have current, updated information on this disease, identifying new endemic areas, manner of exposure to the fungi, and molecular characterization of the causative agents. We report a recent outbreak of histoplasmosis in treasure hunters and the molecular characterization of two isolates obtained from these patients. Methods Six patients admitted to the National Institute of Respiratory Diseases (INER in Mexico City presented severe respiratory symptoms suggestive of histoplasmosis. They acquired the infection in the Veracruz (VZ endemic zone. Diagnosis was made by X-ray and Computed tomography (CT, liver function, immunological techniques, and culture. Identification of H. capsulatum isolates was confirmed by using Polymerase chain reaction (PCR was conducted with a probe from the M antigen, and the isolates were characterized by means of Random amplification of polymorphic DNA (RAPD-PCR employed the 1253 oligonucleotide and a mixture of oligonucleotides 1281 and 1283. These were compared to eight reference strain isolates from neighboring areas. Results X-ray and CT revealed disseminated micronodular images throughout lung parenchyma, as well as bilateral retrocaval, prevascular, subcarinal, and hilar adenopathies, hepatosplenomegaly, and altered liver function tests. Five of the six patients developed disseminated histoplasmosis. Two H. capsulatum strains were isolated. The same band profile was detected in both strains, indicating that both isolates corresponded to the sole H. capsulatum strain. Molecular characterization of the isolates was similar in 100% with the EH-53 Hidalgo human (HG strain (reference strain integrated into the LAm A clade described for

  12. Atypical disseminated cutaneous histoplasmosis in an immunocompetent child, caused by an "aberrant" variant of Histoplasma capsulatum var. capsulatum

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    LACAZ Carlos da Silva

    1999-01-01

    Full Text Available A case of atypical disseminated cutaneous histoplasmosis in a five-year old, otherwise healthy child, native and resident in São Paulo metropolitan area is reported. Cutaneous lesions were clinically atypical. Histologic examination disclosed a granulomatous reaction but no fungal structures could be demonstrated by specific staining nor by immunohistochemical reaction. The fungus was isolated from biopsy material on two different occasions, confirming diagnosis of an unusual fungal infection. The fungus, originally thought to be a Sepedonium sp. due to the large sized, hyaline or brownish colored tuberculated macroconidia and to lack of dimorphism (yeast form at 37 °C produce H and M antigens, visualized by the immunodiffusion with rabbit anti-Histoplasma capsulatum hyperimmune serum. Patient?s serum sample was non reactive with H. capsulatum antigen by immunodiffusion, counterimmunoelectrophoresis and complement fixation tests, and immunoenzymatic assay failed to detect the specific circulating antigen. This serum was tested negative by double immunodiffusion when antigen obtained from one of the isolated samples was used. Both cultures were sent to Dr. Leo Kaufman, Ph.D. (Mycoses Immunodiagnostic Laboratory, CDC-Atlanta/USA, who identified them as H. capsulatum by the exoantigen and gen-probe tests. Both clinic and mycologic characteristics of the present case were atypical, suggesting the fungus isolated is an ?aberrant variant? of H. capsulatum var. capsulatum, as described by SUTTON et al. in 199719. Treatment with itraconazole 100 mg/day led to cure within 90 days

  13. Aislamientos de Histoplasma capsulatum con morfología aberrante obtenidos en la República Argentina Histoplasma capsulatum with aberrant morphology isolated in Argentina

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    M.F. Zuiani

    2006-04-01

    Full Text Available Por primera vez en la Argentina se describe una cepa de Histoplasma capsulatum var. capsulatum con morfotipo aberrante, obtenida de un paciente con SIDA. En los primocultivos desarrollados en agar Sabouraud a 25-28 °C, las colonias de la fase micelial fueron blancas, glabras, umbilicadas, centralmente radiadas y de bordes regulares, más semejantes a las de hongos hialinos ambientales que a las de H. capsulatum. Al examen microscópico llamó la atención la ausencia de conidios característicos, la presencia de clamidoconidios solitarios, terminales e intercalares, de 4 µm de diámetro, y el engrosamiento de las hifas. La identificación del hongo se confirmó mediante la detección de exoantígenos específicos (H y M en los sobrenadantes de cultivos de la fase micelial y por la reversión a la fase levaduriforme típica, obtenida al incubar en agar cerebro-corazón adicionado con cisteína a 37 °C. Mediante RAPD-PCR con los iniciadores 1281-1283, el perfil del ADN genómico coincidió con el genotipo de H. capsulatum predominante entre los aislamientos de pacientes argentinos.For the first time in Argentina, we describe a strain of Histoplasma capsulatum var. capsulatum with an aberrant morphology that was isolated from a single patient with AIDS. Mycelial phase cultures on agar Sabouraud at 25-28 °C showed white, glabrous, umbilicated and centrally radiated colonies. Unusual microscopic findings were the absence of typical conidia, the presence of terminal/intercalary chlamydoconidia with a diameter of 4 µm and of thickened hyphae. Fungal identification was confirmed by the detection of bands H and M species specific antigens in mycelial culture supernatants and reversion to the typical yeast phase on agar brain-heart-cysteine at 37 °C. The genomic DNA profile obtained by RAPD-PCR with primers 1281-1283 coincided with the predominant profile of H.capsulatum among isolates from Argentine patients.

  14. Development and characterization of Histoplasma capsulatum-reactive murine T-cell lines and clones

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    Deepe, George S., Jr.; Smith, James G.; Denman, David; Bullock, Ward E.; Sonnenfeld, Gerald

    1986-01-01

    Several Histoplasma capsulatum-reactive murine cloned T-cell lines (TCLs) were isolated from spleens of C57BL/6 mice immunized with viable H. capsulatum yeast cells, using the methodology of Kimoto and Fathman (1980). These T-cells were characterized phenotypically as Thy-1.2(+) Lyt-1(+) L3T4(+) Lyt-2(-), that is, as the helper/inducer phenotype. The cloned T cells proliferate in response to histoplasmin and, in some cases, to heterologous fungal anigens. Upon injection of mice with the antigen, the T-cells mediate local delayed-type hypersensitivity responses and, after stimulation, release regulatory lymphokines.

  15. Selective photoinactivation of Histoplasma capsulatum by water-soluble derivatives chalcones.

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    Melo, Wanessa C M A; Santos, Mariana Bastos Dos; Marques, Beatriz de Carvalho; Regasini, Luis Octávio; Giannini, Maria José Soares Mendes; Almeida, Ana Marisa Fusco

    2017-06-01

    Histoplasmosis is a respiratory and systemic disease caused by the dimorphic fungus Histoplasma capsulatum. The clinical features may vary from asymptomatic infections to disseminated severe form depending of patient immunity. The treatment of histoplasmosis can be performed with itraconazole, fluconazole, and in the disseminated forms is used amphotericin B. However, the critical side effects of amphotericin B, the cases of itraconazole therapy failure and the appearance of fluconozole-resistant strains makes necessary the search of new strategies to treat this disease. Antimicrobial photodynamic therapy (aPDT) seems to be a potential candidate once have been show efficacy to inhibit others dimorphic fungi. Although the photosensitizer (PS) chalcone aggregates in biological medium, it has antifungal activity and show a high quantum yield of ROS formation. So, the aim of this study was to obtain the experimental parameters to achieve an acceptable selective chalcone water-soluble derivatives photoinactivation of H. capsulatum comparing with fibroblastic and keratinocytes cells which are the constituents of some potential host tissues. Yeast and cells were incubated with the same chalchones concentrations and short incubation time followed by irradiation with equal dose of light. The best conditions to kill H. capsulatum selectively were very low photosensitizers concentration (1.95μgmL(-1)) incubated by 15min and irradiated with LED 450nm with 24Jcm(-2). Key words: chalcone, Histoplasma capsulatum, aPDT, selectivity. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Biological activity of the mite Sancassania sp. (Acari: Acaridae from bat guano associated with the pathogenic fungus Histoplasma capsulatum

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    Daniel A Estrada-Bárcenas

    2010-03-01

    Full Text Available Mites and the mammal pathogenic fungus Histoplasma capsulatum are the major components of bat guano microbiota. Interactions between mites and H. capsulatum were evaluated under laboratory conditions. Acarid mites, mainly Sancassania sp., were the most abundant microarthropod in the sampled guano of the Mexican bat Tadarida brasiliensis mexicana and, based on its morphology, Sancassania sp. was similar to the cosmopolitan species Sancassania sphaerogaster. The mycophagous and vectoring activities of this mite were tested for H. capsulatum and two other fungal species, Sporothrix schenckii (pathogenic and Aspergillus sclerotiorum (non-pathogenic. S. ca. sphaerogaster was able to reproduce in H. capsulatum and S. schenckii colonies, multiplying in great numbers under controlled fungal mycelial-phase culture conditions. H. capsulatum colonies were completely destroyed after 14 days of in vitro interaction with mites. In contrast, S. ca. sphaerogaster did not reproduce in A. sclerotiorum cultures. S. ca. sphaerogaster was found vectoring H. capsulatum, but not the two other fungal species studied.

  17. Electrotransformation and Expression of Bacterial Genes Encoding Hygromycin Phosphotransferase and β-Galactosidase in the Pathogenic Fungus Histoplasma capsulatum

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    Woods, Jon P.; Heinecke, Elizabeth L.; Goldman, William E.

    1998-01-01

    We developed an efficient electrotransformation system for the pathogenic fungus Histoplasma capsulatum and used it to examine the effects of features of the transforming DNA on transformation efficiency and fate of the transforming DNA and to demonstrate fungal expression of two recombinant Escherichia coli genes, hph and lacZ. Linearized DNA and plasmids containing Histoplasma telomeric sequences showed the greatest transformation efficiencies, while the plasmid vector had no significant ef...

  18. Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection

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    Fabrine Sales Massafera Tristão

    2012-04-01

    Full Text Available INTRODUCTION: During histoplasmosis, Histoplasma capsulatum soluble antigens (CFAg can be naturally released by yeast cells. Because CFAg can be specifically targeted during infection, in the present study we investigated CFAg release in experimental murine histoplasmosis, and evaluated the host humoral immune response against high-molecular-mass antigens (hMMAg. >150 kDa, the more immunogenic CFAg fraction. METHODS: Mice were infected with 2.2x10(4 H. capsulatum IMT/HC128 yeast cells. The soluble CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg circulating immune complexes (CIC levels were determined by enzymelinked immunosorbent assay, at days 0, 7, 14, and 28 post-infection. RESULTS: We observed a progressive increase in circulating levels of CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg CIC after H. capsulatum infection. The hMMAg showed a high percentage of carbohydrates and at least two main immunogenic components. CONCLUSIONS: We verified for the first time that hMMAg from H. capsulatum IMT/HC128 strain induce humoral immune response and lead to CIC formation during experimental histoplasmosis.

  19. Primer aislamiento de Histoplasma capsulatum de murciélago urbano Eumops bonariensis First isolation of Histoplasma capsulatum from the urban bat Eumops bonariensis

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    C.E. Canteros

    2005-03-01

    Full Text Available Se comunica el primer aislamiento de Histoplasma capsulatum var. capsulatum de un murciélago macho de la especie Eumops bonariensis, capturado en la ciudad de Buenos Aires en 2003. Los aislamientos fueron recuperados de bazo e hígado e identificados fenotípicamente. Se los comparó por PCR, con 17 aislamientos clínicos, 12 de pacientes residentes en la ciudad de Buenos Aires y cinco de otros países de América, usando los iniciadores 1283, (GTG5, (GACA4 y M13. Con los cuatro iniciadores, los perfiles de los aislamientos de murciélago resultaron idénticos entre sí y más relacionados a los de pacientes de Buenos Aires que a los de otros países (porcentaje de similitud: 91-100% y 55-87%, respectivamente. La alta relación genética entre los aislamientos obtenidos del murciélago y de los humanos residentes en Buenos Aires sugiere una fuente común de infección. Este es el primer registro de E. bonariensis infectado con H. capsulatum en el mundo, y el primer aislamiento del hongo en la población de quirópteros de la Argentina. Así como estos mamíferos actúan como reservorio y dispersan el hongo en la naturaleza, la infección en murciélagos urbanos podría asociarse al elevado número de casos de histoplasmosis entre pacientes inmunodeprimidos en la ciudad de Buenos Aires.We report the first isolation of Histoplasma capsulatum var. capsulatum from a male bat Eumops bonariensis captured in Buenos Aires city in 2003. The pathogen was recovered from spleen and liver specimens, and was identified by its phenotypic characteristics. PCR with primers 1283, (GTG5, (GACA4 and M13 was used to compare both bat isolates with 17 human isolates, 12 from patients residing in Buenos Aires city, and 5 from other countries of the Americas. The profiles obtained with the four primers showed that both bat isolates were identical to each other and closer to Buenos Aires patients than to the other isolates (similarity percentage: 91-100% and 55

  20. Experimental annotation of the human pathogen Histoplasma capsulatum transcribed regions using high-resolution tiling arrays

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    Sil Anita

    2011-09-01

    Full Text Available Abstract Background The fungal pathogen Histoplasma capsulatum is thought to be the most common cause of fungal respiratory infections in immunocompetent humans, yet little is known about its biology. Here we provide the first genome-wide studies to experimentally validate its genome annotation. A functional interrogation of the Histoplasma genome provides critical support for continued investigation into the biology and pathogenesis of H. capsulatum and related fungi. Results We employed a three-pronged approach to provide a functional annotation for the H. capsulatum G217B strain. First, we probed high-density tiling arrays with labeled cDNAs from cells grown under diverse conditions. These data defined 6,172 transcriptionally active regions (TARs, providing validation of 6,008 gene predictions. Interestingly, 22% of these predictions showed evidence of anti-sense transcription. Additionally, we detected transcription of 264 novel genes not present in the original gene predictions. To further enrich our analysis, we incorporated expression data from whole-genome oligonucleotide microarrays. These expression data included profiling under growth conditions that were not represented in the tiling experiment, and validated an additional 2,249 gene predictions. Finally, we compared the G217B gene predictions to other available fungal genomes, and observed that an additional 254 gene predictions had an ortholog in a different fungal species, suggesting that they represent genuine coding sequences. Conclusions These analyses yielded a high confidence set of validated gene predictions for H. capsulatum. The transcript sets resulting from this study are a valuable resource for further experimental characterization of this ubiquitous fungal pathogen. The data is available for interactive exploration at http://histo.ucsf.edu.

  1. Experimental annotation of the human pathogen Histoplasma capsulatum transcribed regions using high-resolution tiling arrays.

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    Voorhies, Mark; Foo, Catherine K; Sil, Anita

    2011-09-29

    The fungal pathogen Histoplasma capsulatum is thought to be the most common cause of fungal respiratory infections in immunocompetent humans, yet little is known about its biology. Here we provide the first genome-wide studies to experimentally validate its genome annotation. A functional interrogation of the Histoplasma genome provides critical support for continued investigation into the biology and pathogenesis of H. capsulatum and related fungi. We employed a three-pronged approach to provide a functional annotation for the H. capsulatum G217B strain. First, we probed high-density tiling arrays with labeled cDNAs from cells grown under diverse conditions. These data defined 6,172 transcriptionally active regions (TARs), providing validation of 6,008 gene predictions. Interestingly, 22% of these predictions showed evidence of anti-sense transcription. Additionally, we detected transcription of 264 novel genes not present in the original gene predictions. To further enrich our analysis, we incorporated expression data from whole-genome oligonucleotide microarrays. These expression data included profiling under growth conditions that were not represented in the tiling experiment, and validated an additional 2,249 gene predictions. Finally, we compared the G217B gene predictions to other available fungal genomes, and observed that an additional 254 gene predictions had an ortholog in a different fungal species, suggesting that they represent genuine coding sequences. These analyses yielded a high confidence set of validated gene predictions for H. capsulatum. The transcript sets resulting from this study are a valuable resource for further experimental characterization of this ubiquitous fungal pathogen. The data is available for interactive exploration at http://histo.ucsf.edu.

  2. Insertional mutagenesis enables cleistothecial formation in a non-mating strain of Histoplasma capsulatum

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    Laskowski Meggan C

    2010-02-01

    Full Text Available Abstract Background Histoplasma capsulatum is a pathogenic ascomycete fungus that rapidly loses mating ability in culture. Loss of mating ability, as well as the organism's low rate of targeted gene replacement, limits techniques available for genetic studies in H. capsulatum. Understanding molecular mechanisms regulating mating in this organism may allow us to reverse or prevent loss of mating in H. capsulatum strains, introducing a variety of classical genetics techniques to the field. We generated a strain, UC1, by insertional mutagenesis of the laboratory strain G217B, and found that UC1 acquired the ability to form mating structures called cleistothecia. The aim of this study was to determine the mechanism by which UC1 gained the ability to form cleistothecia. We also present initial studies demonstrating that UC1 can be used as a tool to determine molecular correlates of mating in H. capsulatum. Results The strain UC1 was found to have increased RNA levels of the mating locus transcription factor (MAT1-1-1, and the putative alpha pheromone (PPG1 compared to G217B. Agrobacterium-mediated transformation and integration of T-DNA from the vector pCB301-GFP-HYG were found to be partially responsible for the increased RNA levels of these genes; however, the site of integration appeared to play the largest role in the strain's ability to form cleistothecia. Silencing HMK1, a putative FUS3/KSS1 homolog, had no effect on cleistothecial production by UC1. Protein kinase C (PKC1 RNA and protein levels were increased in UC1 compared to G217B, and pheromone production was found to be linked with Pkc1 activity in H. capsulatum. Conclusions The site of the T-DNA integration event appears to play the largest role in UC1's ability to form cleistothecia. We show that the UC1 strain can be used as a tool to study cleistothecia production in H. capsulatum by manipulating the strain, or by identifying differences between UC1 and G217B. Using these approaches

  3. The interaction between Histoplasma capsulatum cell wall carbohydrates and host components: relevance in the immunomodulatory role of histoplasmosis.

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    Gorocica, Patricia; Taylor, Maria Lucia; Alvarado-Vásquez, Noé; Pérez-Torres, Armando; Lascurain, Ricardo; Zenteno, Edgar

    2009-05-01

    Histoplasma capsulatum is an intracellular fungal pathogen that causes respiratory and systemic disease by proliferating within phagocytic cells. The binding of H. capsulatum to phagocytes may be mediated by the pathogen's cell wall carbohydrates, glucans, which consist of glucose homo and hetero-polymers and whose glycosydic linkage types differ between the yeast and mycelial phases. The alpha-1,3-glucan is considered relevant for H. capsulatum virulence, whereas the beta-1,3-glucan is antigenic and participates in the modulation of the host immune response. H. capsulatum cell wall components with lectin-like activity seem to interact with the host cell surface, while host membrane lectin-like receptors can recognize a particular fungal carbohydrate ligand. This review emphasizes the relevance of the main H. capsulatum and host carbohydrate-driven interactions that allow for binding and internalization of the fungal cell into phagocytes and its subsequent avoidance of intracellular elimination.

  4. The interaction between Histoplasma capsulatum cell wall carbohydrates and host components: relevance in the immunomodulatory role of histoplasmosis

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    Patricia Gorocica

    2009-05-01

    Full Text Available Histoplasma capsulatum is an intracellular fungal pathogen that causes respiratory and systemic disease by proliferating within phagocytic cells. The binding of H. capsulatum to phagocytes may be mediated by the pathogen's cell wall carbohydrates, glucans, which consist of glucose homo and hetero-polymers and whose glycosydic linkage types differ between the yeast and mycelial phases. The ±-1,3-glucan is considered relevant for H. capsulatum virulence, whereas the ²-1,3-glucan is antigenic and participates in the modulation of the host immune response. H. capsulatum cell wall components with lectin-like activity seem to interact with the host cell surface, while host membrane lectin-like receptors can recognize a particular fungal carbohydrate ligand. This review emphasizes the relevance of the main H. capsulatum and host carbohydrate-driven interactions that allow for binding and internalization of the fungal cell into phagocytes and its subsequent avoidance of intracellular elimination.

  5. Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear (Ursus arctos).

    Science.gov (United States)

    Highland, Margaret A; Chaturvedi, Sudha; Perez, Michael; Steinberg, Howard; Wallace, Roberta

    2011-07-01

    A 33-year-old brown bear (Ursus arctos) was evaluated for chronic cough, partial anorexia, and lethargy in early fall of 2009. Radiographs revealed a generalized increase in interstitial density with focal lung field consolidation and air bronchograms more prevalent in the cranial lung lobes. Tracheal sputum and wash fluid grew mixed bacteria and 2 species of Candida on bacterial and fungal cultures, respectively. Serum was negative for antibodies to Aspergillus, Blastomyces, Coccidioides, and Histoplasma by semiquantitative radial immunodiffusion. Antimicrobial and antifungal treatment was administered. The bear died 1 month after entering hibernation. Gross necropsy revealed coalescent nodules and sheets of firm tan tissue covering pleural surfaces of the thoracic cavity and within pulmonary parenchyma, enlarged mesenteric lymph nodes, and intestinal ulcerations. Histopathology revealed granulomatous inflammation with intrahistiocytic yeast, consistent with Histoplasma organisms, in lung, diaphragm, mesenteric lymph nodes, intestine, and adrenal glands. Molecular analysis performed on DNA isolated from lung tissue, including conventional polymerase chain reaction (PCR) targeting the internal transcribed spacer region for the ribosomal RNA gene complex and real-time PCR targeting the gene encoding a unique region of M specific protein, identified the organism to be 100% identical to Histoplasma capsulatum with an average of 4.9 × 10(7) gene copies per gram of tissue. The present report describes histologic and molecular techniques for diagnosing histoplasmosis.

  6. Úlcera de lengua como presentación del Histoplasma capsulatum Tongue ulceration as a presentation of Histoplasma capsulatum infection

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    E J Carbó Amoroso

    2008-06-01

    Full Text Available La histoplasmosis diseminada progresiva es una enfermedad que se manifiesta como reactivación de una infección latente en pacientes inmunodeprimidos, especialmente en personas con déficit en la inmunidad celular. Existen formas agudas, subagudas y crónicas. Las lesiones focales, en especial úlceras mucocutáneas, predominan en la forma diseminada crónica. Reportamos el caso de una paciente con artritis reumatoidea, que controlaba su patología con fármacos antirreumáticos modificadores de la enfermedad (DMARD, la que consultó por úlcera de lengua como única manifestación de una histoplasmosis diseminada crónica. La histopatología fue compatible y el cultivo positivo para Histoplasma capsulatum. La serología para el HIV fue negativa. Existen pocos casos publicados de pacientes con esta localización atípica en forma aislada, en particular aquellos HIV negativos. El itraconazol y la anfotericina B son las dos drogas más utilizadas para tratar esta enfermedad. Los datos clínicos sobre los nuevos azoles, voriconazol y posaconazol son limitados.The progressive disseminated histoplasmosis is a disease produced by reactivation of latent infection in immunocompromised host, specially in persons with defective cell-mediated immunity. There are acute, subacute and chronic forms in the progressive illness. Focal lesions, specially mucocutaneous ulcers, are most frequent in the chronic disseminated forms. We reported a patient with rheumatoid arthritis treated with disease modifying antirheumatic drug (DMARD, with an ulcer of the tongue as only clinical manifestation of a chronic disseminated histoplasmosis. The histopathology was compatible, and the culture was positive for Histoplasma capsulatum. The serology for the HIV was negative. There are few published cases of this isolated form, particularly in patients with HIV negative serological test. Itraconazole and amphotericin B are the most frequently drugs used for the treatment in this

  7. Sexual variability in Histoplasma capsulatum and its possible distribution: what is going on?

    Science.gov (United States)

    Muniz, Mauro Medeiros; Sousa, Carolina Nascimento; Evangelista Oliveira, Manoel Marques; Pizzini, Claudia Vera; Almeida, Marcos Abreu; Rodríguez-Arellanes, Gabriela; Taylor, Maria Lucia; Zancopé-Oliveira, Rosely Maria

    2014-01-01

    Histoplasma capsulatum is a dimorphic fungal pathogen naturally found in the soil. Inhalation of conidia can result in pulmonary histoplasmosis and, in some cases, causes severe disseminated disease and death. This fungus is an ascomycete that has an anamorphic or asexual stage and a teleomorphic or sexual stage, known as Ajellomyces capsulatus, which results from (+) and (-) mating types. Sexual reproduction is regulated by a specialized genomic region known as the mating-type (MAT1) locus. The mating process in this heterothallic species is represented by isolates that contain only one of the two different MAT1 locus idiomorphs (MAT1-1 or MAT1-2) that have unrelated sequences encoding different transcription factors. In medically important dimorphic pathogens and in most ascomycete molds, one MAT locus idiomorph encodes a high-mobility-group (HMG) box-domain transcription factor, and the other idiomorph encodes an alpha-box domain transcription factor. There is scarce molecular information about H. capsulatum mating type although recombinant population structures have been reported that could occur in nature and this process has been documented in distinct models such as parasites and other fungi. In this review, we shall focus on published studies on H. capsulatum sexuality, and outline the distribution of the two H. capsulatum mating types in Latin America. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012). Copyright © 2013 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.

  8. Galleria mellonella as a model host to study Paracoccidioides lutzii and Histoplasma capsulatum

    Science.gov (United States)

    Thomaz, Luciana; García-Rodas, Rocío; Guimarães, Allan J.; Taborda, Carlos P.; Zaragoza, Oscar; Nosanchuk, Joshua D.

    2013-01-01

    Non-mammalian models have been used to investigate fungal virulence. In this work we have explored the use of Galleria mellonella as an infection model for the pathogenic dimorphic fungi Histoplasma capsulatum and Paracoccidioides lutzii. In mammalian models these fungi cause similar infections, and disease outcomes are influenced by the quantity of the infective inocula. We describe a similar aspect in a G. mellonella model and characterize the pathogenesis features in this system. Infection with P. lutzii or H. capsulatum, in all inoculum used, killed larvae at 25 and 37°C. However, there was a lack of correlation between the number of yeast cells used for infection and the time to larvae death, which may indicate that the fungi induce protective responses in a dynamic manner as the lowest concentrations of fungi induced the most rapid death. For both fungi, the degree of larvae melanization was directly proportional to the inocula size, and this effect was visibly more apparent at 37°C. Histological evaluation of the larvae showed a correlation between the inoculum and granuloma-like formation. Our results suggest that G. mellonella is a potentially useful model to study virulence of dimorphic fungi. PMID:23302787

  9. Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum

    Science.gov (United States)

    Guimarães, Allan Jefferson; Hamilton, Andrew John; de M. Guedes, Herbert Leonel

    2008-01-01

    Histoplasmosis, due to the intracellular fungus Histoplasma capsulatum, can be diagnosed by demonstrating the presence of antibodies specific to the immunodominant M antigen. However, the role of this protein in the pathogenesis of histoplasmosis has not been elucidated. We sought to structurally and immunologically characterize the protein, determine yeast cell surface expression, and confirm catalase activity. A 3D-rendering of the M antigen by homology modeling revealed that the structures and domains closely resemble characterized fungal catalases. We generated monoclonal antibodies (mAbs) to the protein and determined that the M antigen is present on the yeast cell surface and in cell wall/cell membrane preparations. Similarly, we found that the majority of catalase activity was in extracts containing fungal surface antigens and that the M antigen is not significantly secreted by live yeast cells. The mAbs also identified unique epitopes on the M antigen. The localization of the M antigen to the cell surface of H. capsulatum yeast and the characterization of the protein's major epitopes have important implications since it demonstrates that although the protein may participate in protecting the fungus against oxidative stress it is also accessible to host immune cells and antibody. PMID:18927619

  10. Deactivation of the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis using hydrogen peroxide vapor.

    Science.gov (United States)

    Hall, Leslie; Otter, Jonathan A; Chewins, John; Wengenack, Nancy L

    2008-03-01

    Hydrogen peroxide vapour (HPV) has been proposed as an alternative to formaldehyde fumigation for the decontamination of biosafety level (BSL) III laboratories. The aim of this study was to evaluate the efficacy of HPV against the dimorphic fungi Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis. Working inside a class II biological safety cabinet (BSC) within a BSL III laboratory, inocula containing approximately 5-log(10) cfu/ml from the mould form of each organism suspended in RPMI medium were deposited on stainless steel discs and allowed to air dry. The organisms were exposed to HPV inside a BSC using a BIOQUELL ClarusS HPV generator. In three replicate experiments, individual discs were transferred into liquid media at timed intervals during a 105 minute HPV exposure period. Control- and HPV-exposed discs were incubated in RPMI media at 30 degrees C for 6 weeks to determine if any viable organisms remained. Positive cultures were confirmed using specific nucleic acid hybridization probes. Results indicate that H. capsulatum, B. dermatitidis and C. immitis were killed within 30 minutes of HPV exposure.

  11. A Case of Histoplasma capsulatum Causing Granulomatous Liver Disease and Addisonian Crisis

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    P Wong

    2001-01-01

    Full Text Available A 56-year-old man with persistently elevated liver enzyme levels, fatigue, lethargy and a 9.0 kg weight loss over six months underwent a percutaneous liver biopsy that demonstrated multiple granulomas. Screening serologies were positive for histoplasmosis, and he was started on itraconazole treatment. He returned to hospital the same night with coffee-ground emesis and in Addisonian crisis requiring parenteral steroids and intensive care unit support. An abdominal computed tomography scan revealed bilaterally enlarged, nonenhancing adrenal glands suggestive of infarcts, presumed secondary to histoplasmosis. Treatment was initiated with amphotericin B, and Histoplasma capsulatum was cultured from his urine and cerebrospinal fluid. A serum immunodiffusion test was also positive for both H and M bands, indicating active infection with Histoplasmosis species. His serum and urine samples were also weakly positive for the antigen. Despite complications of renal failure, pneumonia and congestive heart failure, he recovered with medical therapy and was discharged home to complete a prolonged course of itraconazole therapy. While hepatic granulomas often reflect an occult disease process, the cause may remain undiscovered in 30% to 50% of patients despite exhaustive investigations. H capsulatum is an uncommon cause of granulomatous liver disease, and with its protean clinical presentation, a high index of suspicion is needed to make the diagnosis and avoid the potentially high fatality rate associated with disseminated infection.

  12. Antibody Binding Alters the Characteristics and Contents of Extracellular Vesicles Released by Histoplasma capsulatum.

    Science.gov (United States)

    Matos Baltazar, Ludmila; Nakayasu, Ernesto S; Sobreira, Tiago J P; Choi, Hyungwon; Casadevall, Arturo; Nimrichter, Leonardo; Nosanchuk, Joshua D

    2016-01-01

    Histoplasma capsulatum produces extracellular vesicles containing virulence-associated molecules capable of modulating host machinery, benefiting the pathogen. Treatment of H. capsulatum cells with monoclonal antibodies (MAbs) can change the outcome of infection in mice. We evaluated the sizes, enzymatic contents, and proteomic profiles of the vesicles released by fungal cells treated with either protective MAb 6B7 (IgG1) or nonprotective MAb 7B6 (IgG2b), both of which bind H. capsulatum heat shock protein 60 (Hsp60). Our results showed that treatment with either MAb was associated with changes in size and vesicle loading. MAb treatments reduced vesicle phosphatase and catalase activities compared to those of vesicles from untreated controls. We identified 1,125 proteins in vesicles, and 250 of these manifested differences in abundance relative to that of proteins in vesicles isolated from yeast cells exposed to Hsp60-binding MAbs, indicating that surface binding of fungal cells by MAbs modified protein loading in the vesicles. The abundance of upregulated proteins in vesicles upon MAb 7B6 treatment was 44.8% of the protein quantities in vesicles from fungal cells treated with MAb 6B7. Analysis of orthologous proteins previously identified in vesicles from other fungi showed that different ascomycete fungi have similar proteins in their extracellular milieu, many of which are associated with virulence. Our results demonstrate that antibody binding can modulate fungal cell responses, resulting in differential loading of vesicles, which could alter fungal cell susceptibility to host defenses. This finding provides additional evidence that antibody binding modulates microbial physiology and suggests a new function for specific immunoglobulins through alterations of fungal secretion. IMPORTANCE Diverse fungal species release extracellular vesicles, indicating that this is a common pathway for the delivery of molecules to the extracellular space. However, there has

  13. Comparison of diagnostic methods to detect Histoplasma capsulatum in serum and blood samples from AIDS patients

    Science.gov (United States)

    da Silva, Marcos Vinicius; Criado, Paulo Ricardo; Luiz, Olinda do Carmo; Vicentini, Adriana Pardini

    2018-01-01

    Background Although early and rapid detection of histoplasmosis is essential to prevent morbidity and mortality, few diagnostic tools are available in resource-limited areas, especially where it is endemic and HIV/AIDS is also epidemic. Thus, we compared conventional and molecular methods to detect Histoplasma capsulatum in sera and blood from HIV/AIDS patients. Methodology We collected a total of 40 samples from control volunteers and patients suspected of histoplasmosis, some of whom were also infected with other pathogens. Samples were then analyzed by mycological, serological, and molecular methods, and stratified as histoplasmostic with (group I) or without AIDS (group II), uninfected (group III), and infected with HIV and other pathogens only (group IV). All patients were receiving treatment for histoplasmosis and other infections at the time of sample collection. Results Comparison of conventional methods with nested PCR using primers against H. capsulatum 18S rRNA (HC18S), 5.8S rRNA ITS (HC5.8S-ITS), and a 100 kDa protein (HC100) revealed that sensitivity against sera was highest for PCR with HC5.8S-ITS, followed by immunoblotting, double immunodiffusion, PCR with HC18S, and PCR with HC100. Specificity was equally high for double immunodiffusion, immunoblotting and PCR with HC100, followed for PCR with HC18S and HC5.8-ITS. Against blood, sensitivity was highest for PCR with HC5.8S-ITS, followed by PCR with HC18S, Giemsa staining, and PCR with HC100. Specificity was highest for Giemsa staining and PCR with HC100, followed by PCR with HC18S and HC5.8S-ITS. PCR was less efficient in patients with immunodeficiency due to HIV/AIDS and/or related diseases. Conclusion Molecular techniques may detect histoplasmosis even in cases with negative serology and mycology, potentially enabling early diagnosis. PMID:29342162

  14. Interleukin-5 mediates peritoneal eosinophilia induced by the F1 cell wall fraction of Histoplasma capsulatum

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    Sá-Nunes A.

    2004-01-01

    Full Text Available An alkali-insoluble fraction 1 (F1, which contains mainly ß-glucan isolated from the cell wall of Histoplasma capsulatum, induces eosinophil recruitment into the peritoneal cavity of mice. The present study was carried out to determine the participation of interleukin-5 (IL-5 in this process. Inbred C57BL/6 male mice weighing 15-20 g were treated ip with 100 µg of anti-IL-5 monoclonal antibody (TRFK-5, N = 7 or an isotype-matched antibody (N = 7, followed by 300 µg F1 in 1 ml PBS ip 24 h later. Controls (N = 5 received only 1 ml PBS. Two days later, cells from the peritoneal cavity were harvested by injection of 3 ml PBS and total cell counts were determined using diluting fluid in a Neubauer chamber. Differential counts were performed using Rosenfeld-stained cytospin preparations. The F1 injection induced significant (P < 0.01 leukocyte recruitment into the peritoneal cavity (8.4 x 10(6 cells/ml when compared with PBS alone (5.5 x 10(6 cells/ml. Moreover, F1 selectively (P < 0.01 induced eosinophil recruitment (1 x 10(6 cells/ml when compared to the control group (0.07 x 10(6 cells/ml. Treatment with TRFK-5 significantly (P < 0.01 inhibited eosinophil recruitment (0.18 x 10(6 cells/ml by F1 without affecting recruitment of mononuclear cells or neutrophils. We conclude that the F1 fraction of the cell wall of H. capsulatum induces peritoneal eosinophilia by an IL-5-dependent mechanism. Depletion of this cytokine does not have effect on the recruitment of other cell types induced by F1.

  15. Detection and characterization of Histoplasma capsulatum in a German badger (Meles meles) by ITS sequencing and multilocus sequencing analysis.

    Science.gov (United States)

    Eisenberg, Tobias; Seeger, Helga; Kasuga, Takao; Eskens, Ulrich; Sauerwald, Claudia; Kaim, Ute

    2013-05-01

    A wild badger (Meles meles) with a severe nodular dermatitis was presented for post mortem examination. Numerous cutaneous granulomas with superficial ulceration were present especially on head, dorsum, and forearms were found at necropsy. Histopathological examination of the skin revealed a severe granulomatous dermatitis with abundant intralesional round to spherical yeast-like cells, 2-5 μm in diameter, altogether consistent with the clinical appearance of histoplasmosis farciminosi. The structures stained positively with Grocott's methenamine silver and Periodic acid-Schiff stains, but attempts to isolate the etiologic agent at 25 and 37°C failed. DNA was directly extracted from tissue samples and the ribosomal genes ITS1-5.8S-ITS2 were partially sequenced. This revealed 99% identity to sequences from Ajellomyces capsulatus, the teleomorph of Histoplasma capsulatum, which was derived from a human case in Japan, as well as from horses from Egypt and Poland. Phylogenetic multi-locus sequence analysis demonstrated that the fungus in our case belonged to the Eurasian clade which contains members of former varieties H. capsulatum var. capsulatum, H. capsulatum var. farciminosum. This is the first study of molecular and phylogenetic aspects of H. capsulatum, as well as evidence for histoplasmosis farciminosi in a badger, further illuminating the role of this rare pathogen in Central Europe.

  16. Erythropoietin Exacerbates Inflammation and Increases the Mortality of Histoplasma capsulatum-Infected Mice

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    Gisele Aparecida Locachevic

    2015-01-01

    Full Text Available Erythropoietin (EPO is a key hormone involved in red blood cell formation, but its effects on nonerythroid cells, such as macrophages, have not been described. Macrophages are key cells in controlling histoplasmosis, a fungal infection caused by Histoplasma capsulatum (Hc. Considering that little is known about EPO’s role during fungal infections and its capacity to activate macrophages, in this study we investigated the impact of EPO pretreatment on the alveolar immune response during Hc infection. The consequence of EPO pretreatment on fungal infection was determined by evaluating animal survival, fungal burden, activation of bronchoalveolar macrophages, inflammatory mediator release, and lung inflammation. Pretreatment with EPO diminished mononuclear cell numbers, increased the recruitment of F4/80+/CD80+ and F4/80+/CD86+ cells to the bronchoalveolar space, induced higher production of IFN-γ, IL-6, MIP-1α, MCP-1, and LTB4, reduced PGE2 concentration, and did not affect fungal burden. As a consequence, we observed an increase in lung inflammation with extensive tissue damage that might account for augmented mouse mortality after infection. Our results demonstrate for the first time that EPO treatment has a deleterious impact on lung immune responses during fungal infection.

  17. Application of the diffusion-convection equation to modeling the infection by histoplasma-capsulatum

    Science.gov (United States)

    Jaime, Sergio A.

    2013-05-01

    Using computer algebra, the respiratory infection of the histoplasma capsulatum fungus was modeled and analyzed; the effects of the infection could also be described as a change in the lungs capacity to expand (associated with its elastic modulus). A further analysis to the immune system was also done in order to describe and model the way the body can handle those kinds of infections once they are hosted in the body. Using those models we can describe the behavior of the respiratory infection and then how to reduce or control its effects. As an investigation in the medical field, we need to test the models obtained and compare the results with the real infection behavior. The models where made based on the diffusive-convective equation; giving some initial and boundary conditions, we can get to the results obtained, which can describe how the infection is spreading and with a previous study of the immune system, the infection control done by the body can also be modeled.

  18. Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.

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    Tzu-Hsuan Chang

    2017-07-01

    Full Text Available Inflammasome is an intracellular protein complex that serves as cytosolic pattern recognition receptor (PRR to engage with pathogens and to process cytokines of the interleukin-1 (IL-1 family into bioactive molecules. It has been established that interleukin-1β (IL-1β is important to host defense against Histoplasma capsulatum infection. However, the detailed mechanism of how H. capsulatum induces inflammasome activation leading to IL-1β production has not been studied. Here, we showed in dendritic cells (DCs that H. capsulatum triggers caspase-1 activation and IL-1β production through NLRP3 inflammasome. By reciprocal blocking of Dectin-1 or Dectin-2 in single receptor-deficient DCs and cells from Clec4n-/-, Clec7a-/-, and Clec7a-/-Clec4n-/- mice, we discovered that while Dectin-2 operates as a primary receptor, Dectin-1 serves as a secondary one for NLRP3 inflammasome. In addition, both receptors trigger Syk-JNK signal pathway to activate signal 1 (pro-IL-1β synthesis and signal 2 (activation of caspase-1. Results of pulmonary infection with H. capsulatum showed that CD103+ DCs are one of the major producers of IL-1β and Dectin-2 and Dectin-1 double deficiency abolishes their IL-1β response to the fungus. While K+ efflux and cathepsin B (but not ROS function as signal 2, viable but not heat-killed H. capsulatum triggers profound lysosomal rupture leading to cathepsin B release. Interestingly, cathepsin B release is regulated by ERK/JNK downstream of Dectin-2 and Dectin-1. Our study demonstrates for the first time the unique roles of Dectin-2 and Dectin-1 in triggering Syk-JNK to activate signal 1 and 2 for H. capsulatum-induced NLRP3 inflammasome activation.

  19. Cryptococcus neoformans and Histoplasma capsulatum in dove's (Columbia livia) excreta in Bolívar state, Venezuela.

    Science.gov (United States)

    Cermeño, Julman R; Hernández, Isabel; Cabello, Ismery; Orellán, Yida; Cermeño, Julmery J; Albornoz, Rosa; Padrón, Elba; Godoy, Gerardo

    2006-01-01

    Dove's excreta samples from state Bolívar several places in Venezuela, were evaluated to determine the presence of primary pathogen fungi in dove's excreta. Filamentous fungi such as: Aspergillus spp (31.1%), Mucor spp (20.2%), Penicillium spp (9.5%) and Fusarium spp (6.7%) were the most frequently isolated strains. Species such as Candida albicans (4.1%), Cryptococcus albidus and Rhodotorula spp (2.7%), C. neoformans var neoformans (1.4%), Trichosporum asahii (1.4%), Curvularia, Microsporum and Phoma as well as Histoplasma capsulatum (1.3%) were less frecuently isolated. This study shows the presence of C. neoformans and H. capsulatum in dove's excreta from Bolívar state, it remarks infection risk with these pathogens fungi and the necessity to avoid accumulation of dove's excreta.

  20. Obtenção de exoantígenos de Histoplasma capsulatum em meio de neopeptona, glicose, tiamina e asparagina (NGTA Histoplasma capsulatum exocellular antigens. Obtention in neopeptone, glucose, thiamine and asparagine medium (NGTA

    Directory of Open Access Journals (Sweden)

    Nilma Maciel Garcia

    1990-10-01

    Full Text Available O presente trabalho teve como objetivo a produção de exoantígenos H e M das amostras 58, B-679, A-811 e O187 de Histoplasma capsulatum, utilizando o meio NGTA (neopeptona, glicose, tiamina e asparagina em períodos de cultivo de 1, 2 e 3 meses, a 36ºC, sob agitação constante (50 v.p.m.. Os antígenos brutos foram avaliados contra anti-soro e antígeno de Histoplasma capsulatum de referência (Center for Disease Control, 4 soros de pacientes portadores de paracoccidioidomicose, 7 de histoplasmose e soro hiperimune anti-H. capsulatum produzido em coelhos, através da reação de imunodifusão dupla. Verificou-se que, com exceção de B-679 com 1 mês de crescimento, todos os demais exoantígenos apresentaram as frações H e M de precipitação. Os exoantígenos obtidos de A-811 apresentaram só a banda H. Excetuando-se os exoantígenos 58 e B-679 com 1 mês de crescimento, todos os demais exoantígenos reagiram contra soros de pacientes com histoplasmose. Em relação aos soros de pacientes com paracoccidioidomicose, somente os exoantígenos 58 e O187 não apresentaram reação cruzada. Todos os exoantígenos reagiram frente ao soro hiperimune de coelho anti-H. capsulatum. Para obtenção de exoantígenos de H. capsulatum, sugerimos que as amostras sejam cultivadas sob as condições anteriormente descritas, adotando-se o período de 3 meses de crescimento, utilizando-se exoantígenos de referência como controles da reação.The purpose of this work is obtaining exocellular antigens H and M from 4 H. capsulatum strains using NGTA medium (neopeptone, glucose, thiamine and asparagine for periods of 1,2 and 3 months, at 36ºC and continuously shaken. The exocellular antigens were evaluated by double immunodiffusion test against H. capsulatum rabbit antiserum, 7 histoplasmosis sera, 4 paracoccidioidomycosis sera and a reference antigen and antibody furnished by C.D.C. (Atlanta - USA. Except for the exocellular antigen from strain B.679 with 1

  1. Evaluation of berries of Phytolacca dodecandra for growth inhibition of Histoplasma capsulatum var. farciminosum and treatment of cases of epizootic lymphangitis in Ethiopia

    OpenAIRE

    Negesse Mekonnen; Eyasu Makonnen; Nigatu Aklilu; Gobena Ameni

    2012-01-01

    Objective: To evaluate the berries of Phytolacca dodecandra (P. dodecandra) for its effect on Histoplasma capsulatum var. farciminosum (HCF) and for the treatment of cases of epizootic lymphangitis (EL). Methods: Samples were collected from un-ruptured nodules of cases of EL at Debre Zeit and Akaki (central Ethiopia). Mycological culture and isolation of HCF were performed at the Aklilu Lemma Institute of Pathobiology. Phytochemical screening was done for n-butanol extract of P. dodecandra...

  2. Investigation of an optimal cell lysis method for the study of the zinc metalloproteome of Histoplasma capsulatum.

    Science.gov (United States)

    Donnell, Anna M; Lewis, Stephanie; Abraham, Sami; Subramanian, Kavitha; Figueroa, Julio Landero; Deepe, George S; Vonderheide, Anne P

    2017-08-12

    This work sought to assess optimal extraction conditions in the study of the metalloproteome of the dimorphic fungus Histoplasma capsulatum. One of the body's responses to H. capsulatum infection is sequestration of zinc within host macrophage (MØ), as reported by Vignesh et al. (Immunity 39:697-710, 2013) and Vignesh et al. (PLOS Pathog 9:E1003815, 2013). Thus, metalloproteins containing zinc were of greatest interest as it plays a critical role in survival of the fungus. One challenge in metalloproteomics is the preservation of the native structure of proteins to retain non-covalently bound metals. Many of the conventional cell lysis, separation, and identification techniques in proteomics are carried out under conditions that could lead to protein denaturation. Various cell lysis techniques were investigated in an effort to both maintain the metalloproteins during lysis and subsequent analysis while, at the same time, serving to be strong enough to break the cell wall, allowing access to cytosolic metalloproteins. The addition of 1% Triton x-100, a non-ionic detergent, to the lysis buffer was also studied. Seven lysis methods were considered and these included: Glass Homogenizer (H), Bead Beater (BB), Sonication Probe (SP), Vortex with 1% Triton x-100 (V, T), Vortex with no Triton x-100 (V, NT), Sonication Bath, Vortex, and 1% Triton x-100 (SB, V, T) and Sonication Bath, Vortex, and no Triton x-100 (SB, V, NT). A Qubit® Assay was used to compare total protein concentration and inductively coupled plasma-mass spectrometry (ICP-MS) was utilized for total metal analysis of cell lysates. Size exclusion chromatography coupled to ICP-MS (SEC-HPLC-ICP-MS) was used for separation of the metalloproteins in the cell lysate and the concentration of Zn over a wide molecular weight range was examined. Additional factors such as potential contamination sources were also considered. A cell lysis method involving vortexing H. capsulatum yeast cells with 500 μm glass beads

  3. Antibody Binding Alters the Characteristics and Contents of Extracellular Vesicles Released by Histoplasma capsulatum

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    Baltazar, Ludmila M.; Nakayasu, Ernesto S.; Sobreira, Tiago; Choi, Hyungwon; Casadevall, Arturo; Nimrichter, Leonardo; Nosanchuk, Joshua D.

    2016-03-30

    ABSTRACT

    Histoplasma capsulatumproduces extracellular vesicles containing virulence-associated molecules capable of modulating host machinery, benefiting the pathogen. Treatment ofH. capsulatumcells with monoclonal antibodies (MAbs) can change the outcome of infection in mice. We evaluated the sizes, enzymatic contents, and proteomic profiles of the vesicles released by fungal cells treated with either protective MAb 6B7 (IgG1) or nonprotective MAb 7B6 (IgG2b), both of which bindH. capsulatumheat shock protein 60 (Hsp60). Our results showed that treatment with either MAb was associated with changes in size and vesicle loading. MAb treatments reduced vesicle phosphatase and catalase activities compared to those of vesicles from untreated controls. We identified 1,125 proteins in vesicles, and 250 of these manifested differences in abundance relative to that of proteins in vesicles isolated from yeast cells exposed to Hsp60-binding MAbs, indicating that surface binding of fungal cells by MAbs modified protein loading in the vesicles. The abundance of upregulated proteins in vesicles upon MAb 7B6 treatment was 44.8% of the protein quantities in vesicles from fungal cells treated with MAb 6B7. Analysis of orthologous proteins previously identified in vesicles from other fungi showed that different ascomycete fungi have similar proteins in their extracellular milieu, many of which are associated with virulence. Our results demonstrate that antibody binding can modulate fungal cell responses, resulting in differential loading of vesicles, which could alter fungal cell susceptibility to host defenses. This finding provides additional evidence that antibody binding modulates microbial physiology and suggests a new function for specific immunoglobulins through alterations of fungal secretion.

    IMPORTANCEDiverse fungal species release extracellular vesicles, indicating that this is a

  4. Molecular detection of Histoplasma capsulatum in the lung of a free-ranging common noctule (Nyctalus noctula) from France using the Hcp100 gene.

    Science.gov (United States)

    González-González, Antonio Ernesto; Ramírez, José Antonio; Aliouat-Denis, Cécile Marie; Demanche, Christine; Aliouat, El Moukhtar; Dei-Cas, Eduardo; Chabé, Magali; Taylor, Maria Lucia

    2013-03-01

    Histoplasma capsulatum is a dimorphic fungus that is widely distributed in the tropical or subtropical areas of the world and infects several mammalian hosts, mainly bats. Infective propagules grow in bat and bird droppings. A specific molecular marker, a highly sensitive fragment of a co-activator protein-coding gene (Hcp100), was used to detect H. capsulatum in lung samples of wild and captive bats from France using a nested polymerase chain reaction. To determine whether bats in France are potential carriers of H. capsulatum, 83 bats were sampled from two regions in France. Sixty-one specimens belonging to the Pteropus rodricensis (n = 45) and Rousettus aegyptiacus (n = 16) species were collected from a zoologic park (La Palmyre, western France). Twenty-two specimens were recovered from the Natural History Museum (Bourges) including the species Plecotus austriacus (n = 1), Pipistrellus pipistrellus (n = 3), and Nyctalus noctula (n = 18). From the lung DNA samples of 83 dead bats, only one sample of an N. noctula bat from Bourges amplified the H. capsulatum Hcp100 marker. The amplified product was sequenced and revealed a high similarity to the G217B H. capsulatum reference strain sequence that was deposited in the GenBank database. This finding suggests that H. capsulatum is an environmental pathogen in France that may infect bats.

  5. Histoplasma capsulatum sinusitis: Possible way of revelation to the disseminated form of histoplasmosis in HIV patients: Case report and literature review.

    Science.gov (United States)

    Elansari, R; Abada, R; Rouadi, S; Roubal, M; Mahtar, M

    2016-01-01

    Histoplasma capsulatum is rarely found in nose and sinuses in immunocompetent and even in immunosupressed patients. A literature review revealed rare cases of H. capsulatum sinusitis and the case we describe in this paper is the first case of H. capsulatum sinusitis in Morocco. The purpose of our work is to present a rare case of H. capsulatum sinusitis and a literature review. A male patient, at his 39 presented to the emergency with suffered from nasal congestion and yellow postnasal rhinorrhea, occasional headaches and epistaxis for over than two months. He had human immunodeficiency virus (HIV) infection for 6 years with failing treatment adherence and he had an opportunistic infection and unconfirmed pulmonary tuberculosis treated 3 years ago. Computerized tomography from paranasal sinus revealed complete opacification of the ethmoid-maxillary sinuses in the right fossa, consistent with acute sinusitis. The cultures of the sinus aspirate, skin biopsy, were positive for H. capsulatum and the histology of the nasal mucosa and skin biopsy specimen of the rash evoking a sinusitis and cutaneous histoplasmosis. The patient was started a treatment with intravenous amphotericin B at a rate of 1 mg/kg/day with clear clinical and biological improvement. Cultures and histopathologic study confirm histoplasmosis. Itraconazole and amphotericin B are the first line drugs. Patients with progressive disseminated histoplasmosis and those with AIDS should be treated with amphotericin B, and the ENT should suspect of opportunistic agents in immunosupressed patients with sinusitis. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  6. Contribution to the study of the mycobiota present in the natural habitats of Histoplasma capsulatum: an integrative study in Guerrero, Mexico Contribución al conocimiento de la micobiota presente en los hábitats naturales de Histoplasma capsulatum: un estudio integral en Guerrero, México

    OpenAIRE

    Miguel Ulloa; Patricia Lappe; Samuel Aguilar; Houng Park; Amelia Pérez-Mejía; Conchita Toriello; Maria Lucia Taylor

    2006-01-01

    The mycobiota present in natural habitats of Histoplasma capsulatum was determined in samples of bat guano, poultry droppings, and intestinal contents of bats. The following fungi were isolated: 1) from bat guano: the ascomycetes Aphanoascus fulvescens, Gymnascella citrina, Gymnoascus dankaliensis, and Chaetomidium fimeti; the mitosporic fungi Aspergillus flavo-furcatis, A. terreus, A. terreus var. aureus, Penicillium spp., Malbranchea aurantiaca, and Sporothrix sp.; and the yeasts Candida ca...

  7. Profile of cytokines in the lungs of BALB/c mice after intra-nasal infection with Histoplasma capsulatum mycelial propagules.

    Science.gov (United States)

    Sahaza, Jorge Humberto; Suárez-Alvarez, Roberto; Estrada-Bárcenas, Daniel Alfonso; Pérez-Torres, Armando; Taylor, Maria Lucia

    2015-08-01

    The host pulmonary response to the fungus Histoplasma capsulatum was evaluated, through the profile of cytokines detected by the MagPix magnetic beads platform in lung homogenates and by lung-granulomas formation, from mice intra-nasally infected with mycelial propagules (M-phase) of two virulent H. capsulatum strains, EH-46 and G-217B. Results highlight that mice lung inflammatory response depends on the H. capsulatum strain used, during the first step of the fungal infection. IL-1β and TNF-α increased their concentrations in mice infected with both strains. The highest levels of IL-6, IL-17, and IL-23 were found in EH-46-infected mice, whereas levels of IL-22 were variable at all post-infection times for both strains. Significant increases of IL-12, IFN-γ, IL-4, and IL-10 were associated to EH-46-infected mice. Histological lung findings from EH-46-infected mice revealed incipient and numerous well-developed granulomas, distributed in lung-lobes at the 14th and the 21st days after infection, according to cytokine profiles. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Detection of Histoplasma capsulatum from clinical specimens by cycling probe-based real-time PCR and nested real-time PCR.

    Science.gov (United States)

    Muraosa, Yasunori; Toyotome, Takahito; Yahiro, Maki; Watanabe, Akira; Shikanai-Yasuda, Maria Aparecida; Kamei, Katsuhiko

    2016-05-01

    We developed new cycling probe-based real-time PCR and nested real-time PCR assays for the detection of Histoplasma capsulatum that were designed to detect the gene encoding N-acetylated α-linked acidic dipeptidase (NAALADase), which we previously identified as an H. capsulatum antigen reacting with sera from patients with histoplasmosis. Both assays specifically detected the DNAs of all H. capsulatum strains but not those of other fungi or human DNA. The limited of detection (LOD) of the real-time PCR assay was 10 DNA copies when using 10-fold serial dilutions of the standard plasmid DNA and 50 DNA copies when using human serum spiked with standard plasmid DNA. The nested real-time PCR improved the LOD to 5 DNA copies when using human serum spiked with standard plasmid DNA, which represents a 10-fold higher than that observed with the real-time PCR assay. To assess the ability of the two assays to diagnose histoplasmosis, we analyzed a small number of clinical specimens collected from five patients with histoplasmosis, such as sera (n = 4), formalin-fixed paraffin-embedded (FFPE) tissue (n = 4), and bronchoalveolar lavage fluid (BALF) (n = 1). Although clinical sensitivity of the real-time PCR assay was insufficiently sensitive (33%), the nested real-time PCR assay increased the clinical sensitivity (77%), suggesting it has a potential to be a useful method for detecting H. capsulatum DNA in clinical specimens. © The Author 2015. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  9. A Multiplex Real-Time PCR Assay for Identification of Pneumocystis jirovecii, Histoplasma capsulatum, and Cryptococcus neoformans/Cryptococcus gattii in Samples from AIDS Patients with Opportunistic Pneumonia

    Science.gov (United States)

    Gago, Sara; Esteban, Cristina; Valero, Clara; Zaragoza, Óscar; Puig de la Bellacasa, Jorge

    2014-01-01

    A molecular diagnostic technique based on real-time PCR was developed for the simultaneous detection of three of the most frequent causative agents of fungal opportunistic pneumonia in AIDS patients: Pneumocystis jirovecii, Histoplasma capsulatum, and Cryptococcus neoformans/Cryptococcus gattii. This technique was tested in cultured strains and in clinical samples from HIV-positive patients. The methodology used involved species-specific molecular beacon probes targeted to the internal transcribed spacer regions of the rDNA. An internal control was also included in each assay. The multiplex real-time PCR assay was tested in 24 clinical strains and 43 clinical samples from AIDS patients with proven fungal infection. The technique developed showed high reproducibility (r2 of >0.98) and specificity (100%). For H. capsulatum and Cryptococcus spp., the detection limits of the method were 20 and 2 fg of genomic DNA/20 μl reaction mixture, respectively, while for P. jirovecii the detection limit was 2.92 log10 copies/20 μl reaction mixture. The sensitivity in vitro was 100% for clinical strains and 90.7% for clinical samples. The assay was positive for 92.5% of the patients. For one of the patients with proven histoplasmosis, P. jirovecii was also detected in a bronchoalveolar lavage sample. No PCR inhibition was detected. This multiplex real-time PCR technique is fast, sensitive, and specific and may have clinical applications. PMID:24478409

  10. Evolution of the mating type locus: insights gained from the dimorphic primary fungal pathogens Histoplasma capsulatum, Coccidioides immitis, and Coccidioides posadasii.

    Science.gov (United States)

    Fraser, James A; Stajich, Jason E; Tarcha, Eric J; Cole, Garry T; Inglis, Diane O; Sil, Anita; Heitman, Joseph

    2007-04-01

    Sexual reproduction of fungi is governed by the mating type (MAT) locus, a specialized region of the genome encoding key transcriptional regulators that direct regulatory networks to specify cell identity and fate. Knowledge of MAT locus structure and evolution has been considerably advanced in recent years as a result of genomic analyses that enable the definition of MAT locus sequences in many species as well as provide an understanding of the evolutionary plasticity of this unique region of the genome. Here, we extend this analysis to define the mating type locus of three dimorphic primary human fungal pathogens, Histoplasma capsulatum, Coccidioides immitis, and Coccidioides posadasii, using genomic analysis, direct sequencing, and bioinformatics. These studies provide evidence that all three species possess heterothallic bipolar mating type systems, with isolates encoding either a high-mobility-group (HMG) domain or an alpha-box transcriptional regulator. These genes are intact in all loci examined and have not been subject to loss or decay, providing evidence that the loss of fertility upon passage in H. capsulatum is not attributable to mutations at the MAT locus. These findings also suggest that an extant sexual cycle remains to be defined in both Coccidioides species, in accord with population genetic evidence. Based on these MAT sequences, a facile PCR test was developed that allows the mating type to be rapidly ascertained. Finally, these studies highlight the evolutionary forces shaping the MAT locus, revealing examples in which flanking genes have been inverted or subsumed and incorporated into an expanding MAT locus, allowing us to propose an expanded model for the evolution of the MAT locus in the phylum Ascomycota.

  11. Evolution of the Mating Type Locus: Insights Gained from the Dimorphic Primary Fungal Pathogens Histoplasma capsulatum, Coccidioides immitis, and Coccidioides posadasii▿ †

    Science.gov (United States)

    Fraser, James A.; Stajich, Jason E.; Tarcha, Eric J.; Cole, Garry T.; Inglis, Diane O.; Sil, Anita; Heitman, Joseph

    2007-01-01

    Sexual reproduction of fungi is governed by the mating type (MAT) locus, a specialized region of the genome encoding key transcriptional regulators that direct regulatory networks to specify cell identity and fate. Knowledge of MAT locus structure and evolution has been considerably advanced in recent years as a result of genomic analyses that enable the definition of MAT locus sequences in many species as well as provide an understanding of the evolutionary plasticity of this unique region of the genome. Here, we extend this analysis to define the mating type locus of three dimorphic primary human fungal pathogens, Histoplasma capsulatum, Coccidioides immitis, and Coccidioides posadasii, using genomic analysis, direct sequencing, and bioinformatics. These studies provide evidence that all three species possess heterothallic bipolar mating type systems, with isolates encoding either a high-mobility-group (HMG) domain or an α-box transcriptional regulator. These genes are intact in all loci examined and have not been subject to loss or decay, providing evidence that the loss of fertility upon passage in H. capsulatum is not attributable to mutations at the MAT locus. These findings also suggest that an extant sexual cycle remains to be defined in both Coccidioides species, in accord with population genetic evidence. Based on these MAT sequences, a facile PCR test was developed that allows the mating type to be rapidly ascertained. Finally, these studies highlight the evolutionary forces shaping the MAT locus, revealing examples in which flanking genes have been inverted or subsumed and incorporated into an expanding MAT locus, allowing us to propose an expanded model for the evolution of the MAT locus in the phylum Ascomycota. PMID:17337636

  12. Evaluation of berries of Phytolacca dodecandra for growth inhibition of Histoplasma capsulatum var. farciminosum and treatment of cases of epizootic lymphangitis in Ethiopia.

    Science.gov (United States)

    Mekonnen, Negesse; Makonnen, Eyasu; Aklilu, Nigatu; Ameni, Gobena

    2012-07-01

    To evaluate the berries of Phytolacca dodecandra (P. dodecandra) for its effect on Histoplasma capsulatum var. farciminosum (HCF) and for the treatment of cases of epizootic lymphangitis (EL). Samples were collected from un-ruptured nodules of cases of EL at Debre Zeit and Akaki (central Ethiopia). Mycological culture and isolation of HCF were performed at the Aklilu Lemma Institute of Pathobiology. Phytochemical screening was done for n-butanol extract of P. dodecandra to detect alkaloids, saponins, phenolic compounds and flavonoids. The minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of aqueous and n-butanol extracts of P. dodecandra against HCF were determined by agar dilution assay. For the in vivo trial, 5% simple ointment was prepared from n-butanol extract and applied topically to 24 (twelve early and twelve moderate) cases of EL. Phytochemical screening showed that n-butanol extract of P. dodecandra was positive for alkaloids, saponins and phenolic compounds but negative for flavonoids. The MICs of n-butanol and aqueous extracts of P. dodecandra were (0.039%-0.078%) and (0.625%-1.250%), respectively. The MFCs of n-butanol and aqueous extracts of P. dodecandra were (0.078%-0.156%) and (1.250%-2.500%), respectively. The MIC and MFC of ketoconazole (positive control) was (1.200×10(-5)%-2.500×10(-5)%) and (5.000×10(-5)%-1.000×10(-4)%), respectively while growth was observed on free medium (negative control). From the total of 24 treated cases of EL, 14 (58.3%) responded to treatment; however, 10 (41.7%) did not respond to treatment. There was no significant difference in the degree of response to treatment between early and moderate cases (χ(2)=0.686; P=0.408). It can be concluded that n-butanol extract of P. dodecandra demonstrates antifungal effects while the aqueous extract shows no antifungal activity.

  13. Contribution to the study of the mycobiota present in the natural habitats of Histoplasma capsulatum: an integrative study in Guerrero, Mexico Contribución al conocimiento de la micobiota presente en los hábitats naturales de Histoplasma capsulatum: un estudio integral en Guerrero, México

    Directory of Open Access Journals (Sweden)

    Miguel Ulloa

    2006-12-01

    Full Text Available The mycobiota present in natural habitats of Histoplasma capsulatum was determined in samples of bat guano, poultry droppings, and intestinal contents of bats. The following fungi were isolated: 1 from bat guano: the ascomycetes Aphanoascus fulvescens, Gymnascella citrina, Gymnoascus dankaliensis, and Chaetomidium fimeti; the mitosporic fungi Aspergillus flavo-furcatis, A. terreus, A. terreus var. aureus, Penicillium spp., Malbranchea aurantiaca, and Sporothrix sp.; and the yeasts Candida catenulata, C. ciferrii, C. famata var. flareri, C. guilliermondii var. guilliermondii, and Rhodotorula spp. 2 from poultry droppings: the coelomycete Phoma sp.; and the yeasts C. albicans, C. catenulata, C. ciferrii, C. famata var. flareri, C. tropicalis, Cryptococcus albidus, Trichosporon moniliiforme, and Trichosporon spp. 3 from the intestinal contents of insectivorous, hematophagous, nectarivorous, and frugivorous bats: Ch. fimeti; the mitosporic fungi Aspergillus candidus, A. flavo-furcatis, A. sulphureus, A. sydowii, A. terreus, A. versicolor, Aspergillus sp., M. aurantiaca, Gliomastix murorum, and Scopulariopsis sp.; and C. famata var. flareri, C. lipolytica, Cr. albidus, and Trichosporon spp. Most of the species found are first records for these substrata and environments in Mexico. The coexistence with H. capsulatum was demonstrated by high specific antibody titers in ELISA serological method, using sera from BALB/c mice previously inoculated with the supernatant of the different samples studied.Se determinó la micobiota presente en diferentes hábitats naturales de Histoplasma capsulatum, como guano de murciélago, excretas de aves de corral, y contenido intestinal de murciélagos. Se aislaron: 1 de guano: los ascomicetes Aphanoascus fulvescens, Gymnascella citrina, Gymnoascus dankaliensis, y Chaetomidium fimeti; los hongos mitospóricos Aspergillus flavo-furcatis, A. terreus, A. terreus var. aureus, Penicillium spp., Malbranchea aurantiaca, y

  14. Worldwide Phylogenetic Distributions and Population Dynamics of the Genus Histoplasma.

    Science.gov (United States)

    Teixeira, Marcus de M; Patané, José S L; Taylor, Maria L; Gómez, Beatriz L; Theodoro, Raquel C; de Hoog, Sybren; Engelthaler, David M; Zancopé-Oliveira, Rosely M; Felipe, Maria S S; Barker, Bridget M

    2016-06-01

    Histoplasma capsulatum comprises a worldwide complex of saprobiotic fungi mainly found in nitrogen/phosphate (often bird guano) enriched soils. The microconidia of Histoplasma species may be inhaled by mammalian hosts, and is followed by a rapid conversion to yeast that can persist in host tissues causing histoplasmosis, a deep pulmonary/systemic mycosis. Histoplasma capsulatum sensu lato is a complex of at least eight clades geographically distributed as follows: Australia, Netherlands, Eurasia, North American classes 1 and 2 (NAm 1 and NAm 2), Latin American groups A and B (LAm A and LAm B) and Africa. With the exception of the Eurasian cluster, those clades are considered phylogenetic species. Increased Histoplasma sampling (n = 234) resulted in the revision of the phylogenetic distribution and population structure using 1,563 aligned nucleotides from four protein-coding regions. The LAm B clade appears to be divided into at least two highly supported clades, which are geographically restricted to either Colombia/Argentina or Brazil respectively. Moreover, a complex population genetic structure was identified within LAm A clade supporting multiple monophylogenetic species, which could be driven by rapid host or environmental adaptation (~0.5 MYA). We found two divergent clades, which include Latin American isolates (newly named as LAm A1 and LAm A2), harboring a cryptic cluster in association with bats. At least six new phylogenetic species are proposed in the Histoplasma species complex supported by different phylogenetic and population genetics methods, comprising LAm A1, LAm A2, LAm B1, LAm B2, RJ and BAC-1 phylogenetic species. The genetic isolation of Histoplasma could be a result of differential dispersion potential of naturally infected bats and other mammals. In addition, the present study guides isolate selection for future population genomics and genome wide association studies in this important pathogen complex.

  15. Comparison of Different DNA-Based Methods for Molecular Typing of Histoplasma capsulatum▿

    Science.gov (United States)

    Muniz, Mauro de Medeiros; Morais e Silva Tavares, Patrícia; Meyer, Wieland; Nosanchuk, Joshua Daniel; Zancope-Oliveira, Rosely Maria

    2010-01-01

    Histoplasma capsulatum is very prevalent in the environment and is one of the most common causes of mycoses in humans and diverse animals in Brazil. Multiple typing methods have been developed to study H. capsulatum epidemiology; however, there is limited information concerning comparisons of results obtained with different methods using the same set of isolates. To explore the diversity of H. capsulatum in Brazil and to determine correlations between the results of three different molecular typing techniques, we examined 51 environmental, animal, and human isolates by M13 PCR fingerprinting, PCR-restriction fragment length polymorphism (RFLP) analysis of the internal transcribed region 1 (ITS1)-5.8S-ITS2 region of the rDNA locus, and DNA sequencing and phylogenetic analysis of parts of four protein-encoding genes, the Arf (ADP ribosylation factor), H-anti (H antigen precursor), Ole (delta-9 fatty acid desaturase), and Tub1 (alpha-tubulin) genes. Each method identified three major genetic clusters, and there was a high level of concordance between the results of the typing techniques. The M13 PCR fingerprinting and PCR-RFLP analyses produced very similar results and separated the H. capsulatum isolates included in this study into three major groups. An additional approach used was comparison of our Brazilian ITS1-5.8S-ITS2 sequences with the sequences deposited previously in NCBI data banks. Our analyses suggest that H. capsulatum can be divided into different molecular types that are dispersed around the world. Our results indicate that the three methods used in this study are reliable and reproducible and that they have similar sensitivities. However, M13 PCR fingerprinting has some advantages over the other two methods as it is faster, cheaper, and more user friendly, which especially increases its utility for molecular typing of Histoplasma in situations where laboratory facilities are relatively limited. PMID:20453140

  16. Histoplasma capsulatum sinusitis: Possible way of revelation to the disseminated form of histoplasmosis in HIV patients

    Directory of Open Access Journals (Sweden)

    R. Elansari

    2016-01-01

    Conclusion: Patients with progressive disseminated histoplasmosis and those with AIDS should be treated with amphotericin B, and the ENT should suspect of opportunistic agents in immunosupressed patients with sinusitis.

  17. Options for salinity mitigation in the Murray-Darling Basin

    OpenAIRE

    Schrobback, Peggy; Adamson, David; Quiggin, John

    2008-01-01

    The Murray-Darling Basin faces increasing pressure on water quantity and quality. In 2006-07, salt interception schemes implemented as part of the Murray–Darling Basin Salinity Management strategy removed over 470,000 tonnes of salt from the water supply, reducing the salinity of water flowing to Adelaide by about 200 EC units. However, the costs of salinity mitigation schemes are increasing. With possible continuing declines in average inflows, costs of salinity and salinity mitigation are...

  18. Persistence of histoplasma in adrenals 7 years after antifungal therapy

    Directory of Open Access Journals (Sweden)

    Deepak Kothari

    2013-01-01

    Full Text Available Adrenal histoplasmosis is an uncommon cause for adrenal insufficiency. The duration of treatment for adrenal histoplasmosis is not clear. Existing treatment regimens advocate antifungals given for periods ranging from 6 months to 2 years. We report here a rare case who showed persistence of histoplasma in adrenal biopsy 7 years after being initially treated with itraconazole for 9 months. This calls for a prolonged therapy with regular review of adrenal morphology and histology in these patients.

  19. Genetic diversity of Histoplasma and Sporothrix complexes based on sequences of their ITS1-5.8S-ITS2 regions from the BOLD System.

    Science.gov (United States)

    Estrada-Bárcenas, Daniel Alfonso; Vite-Garín, Tania; Navarro-Barranco, Hortensia; de la Torre-Arciniega, Raúl; Pérez-Mejía, Amelia; Rodríguez-Arellanes, Gabriela; Ramirez, Jose Antonio; Humberto Sahaza, Jorge; Taylor, Maria Lucia; Toriello, Conchita

    2014-01-01

    High sensitivity and specificity of molecular biology techniques have proven usefulness for the detection, identification and typing of different pathogens. The ITS (Internal Transcribed Spacer) regions of the ribosomal DNA are highly conserved non-coding regions, and have been widely used in different studies including the determination of the genetic diversity of human fungal pathogens. This article wants to contribute to the understanding of the intra- and interspecific genetic diversity of isolates of the Histoplasma capsulatum and Sporothrix schenckii species complexes by an analysis of the available sequences of the ITS regions from different sequence databases. ITS1-5.8S-ITS2 sequences of each fungus, either deposited in GenBank, or from our research groups (registered in the Fungi Barcode of Life Database), were analyzed using the maximum likelihood (ML) method. ML analysis of the ITS sequences discriminated isolates from distant geographic origins and particular wild hosts, depending on the fungal species analyzed. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012). Copyright © 2013 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.

  20. Water reform in the Murray-Darling Basin

    Science.gov (United States)

    Connell, Daniel; Grafton, R. Quentin

    2011-12-01

    In Australia's Murray-Darling Basin the Australian and state governments are attempting to introduce a system of water management that will halt ongoing decline in environmental conditions and resource security and provide a robust foundation for managing climate change. This parallels similar efforts being undertaken in regions such as southern Africa, the southern United States, and Spain. Central to the project is the Australian government's Water Act 2007, which requires the preparation of a comprehensive basin plan expected to be finalized in 2011. This paper places recent and expected developments occurring as part of this process in their historical context and examines factors that could affect implementation. Significant challenges to the success of the basin plan include human resource constraints, legislative tensions within the Australian federal system, difficulties in coordinating the network of water-related agencies in the six jurisdictions with responsibilities in the Murray-Darling Basin, and social, economic, and environmental limitations that restrict policy implementation.

  1. Toward 21st Century Supports: An Interview with Linda Darling-Hammond

    Science.gov (United States)

    Umphrey, Jan

    2009-01-01

    This article presents an interview with Linda Darling-Hammond who is the Charles Ducommun Professor of Education at Stanford University, the director of the Stanford Center for Opportunity Policy and Education, and the co-director of the school redesign network at Stanford. In this interview, Darling-Hammond shares her thoughts on how issues of…

  2. J.N. "Ding" Darling National Wildlife Refuge: Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on J.N. "Ding" Darling NWR for the next 15 years. This plan outlines the Refuge vision and...

  3. J.N. "Ding" Darling National Wildlife Refuge Wilderness Character Monitoring Back-end Database

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This is the back-end data file for the J.N. "Ding" Darling Wilderness Character Monitoring Application. User interface and lookup databases are required for use (see...

  4. Histoplasma capsulatum fungemia in patients with acquired immunodeficiency syndrome: detection by lysis-centrifugation blood-culturing technique Fungemia por Histoplasma capsulatum em pacientes com a síndrome da imunodeficiência adquirida: detecção através da técnica de hemocultivo por lise-centrifugação

    Directory of Open Access Journals (Sweden)

    Flávio de Mattos Oliveira

    2007-06-01

    Full Text Available Progressive disseminated histoplasmosis (PDH is an increasingly common cause of infection in patients with acquired immune deficiency syndrome (AIDS. We report 21 cases of PDH associated with AIDS diagnosed by lysis-centrifugation blood culture method. The most prevalent clinical findings were fever, weight loss, respiratory symptoms, and mucocutaneous lesions. Chest roentgenogram showed diffuse pulmonary infiltrates in 13 of 21 patients (62%. Brochoalveolar fluid has yelded positive culture in four patients only in medium with cycloheximide.Histoplasmose progressiva disseminada (HPD tem aumentado e é causa comum de infecção em pacientes com síndrome da imunodeficiência adquirida (Aids. Relatamos 21 casos de HPD associado com Aids diagnosticada pela técnica de hemocultivo por lise-centrifugação. Os achados clínicos mais prevalentes foram febre, perda de peso, sintomas respiratórios e lesões mucocutâneas. Raios X de tórax mostrou infiltrados pulmonares difusos em 13 dos 21 pacientes (62%. Amostras de lavado broncoalveolar foram positivos em apenas 4 pacientes através de meio com cicloheximida.

  5. 76 FR 16637 - J.N. “Ding” Darling NWR, Lee County, FL; Final Comprehensive Conservation Plan and Finding of No...

    Science.gov (United States)

    2011-03-24

    ... Fish and Wildlife Service J.N. ``Ding'' Darling NWR, Lee County, FL; Final Comprehensive Conservation... impact (FONSI) for the environmental assessment for J.N. ``Ding'' Darling National Wildlife Refuge (NWR... INFORMATION: Introduction With this notice, we finalize the CCP process for J.N. ``Ding'' Darling NWR. We...

  6. Current Issues in Teacher Education: An Interview with Dr. Linda Darling-Hammond

    Science.gov (United States)

    Martin, Linda E.; Mulvihill, Thalia M.

    2017-01-01

    Dr. Linda Darling-Hammond is the Charles E. Ducommun Professor of Education Emeritus at the Stanford Graduate School of Education at Stanford University. She is former president of the American Educational Research Association (AERA) and member of the National Academy of Education and the American Academy of Arts and Sciences. She also served as a…

  7. The Challenges of Supporting New Teachers: A Conversation with Linda Darling-Hammond

    Science.gov (United States)

    Scherer, Marge

    2012-01-01

    In this wide-ranging interview with Educational Leadership, Stanford University Professor of Education Linda Darling-Hammond discusses the kind of preparation and support new teachers need to survive their critical first years in the classroom. Among her central recommendations are more intensive mentoring that lasts through the first year of…

  8. Darling üritab mõjutada britte raha kulutama / Heiki Suurkask

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2008-01-01

    Suurbritannia valitsus on saavutanud suuremate pankadega kokkuleppe, et kodulaenude võlgnevusi talutakse kaks aastat, enne kui hakatakse kinnisvara võõrandama. Rahandusminister Alistair Darling teatas, et ollakse valmis astuma järgmisi samme, et vastutustundlikku laenamist uuesti käima panna. Baasintressimäärade alandamisest; kinnisvarahindade langusest Suurbritannias. Lisa: Austraalia jagab jõulude eel raha

  9. J. N. "Ding" Darling National Wildlife Refuge and The Florida Gulf Refuges - Annual Narrative Report Calendar Year 1995

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual narrative report for J. N. Ding Darling NWR and The Florida Gulf Refuges summarizes Refuge activities during the 1995 calendar year. The report contains...

  10. Discovery of a novel (R-selective bacterial hydroxynitrile lyase from Acidobacterium capsulatum

    Directory of Open Access Journals (Sweden)

    Romana Wiedner

    2014-06-01

    Full Text Available Hydroxynitrile lyases (HNLs are powerful carbon–carbon bond forming enzymes. The reverse of their natural reaction – the stereoselective addition of hydrogen cyanide (HCN to carbonyls – yields chiral cyanohydrins, versatile building blocks for the pharmaceutical and chemical industry. Recently, bacterial HNLs have been discovered, which represent a completely new type: HNLs with a cupin fold. Due to various benefits of cupins (e.g. high yield recombinant expression in Escherichia coli, the class of cupin HNLs provides a new source for interesting, powerful hydroxynitrile lyases in the ongoing search for HNLs with improved activity, enantioselectivity, stability and substrate scope. In this study, database mining revealed a novel cupin HNL from Acidobacterium capsulatum ATCC 51196 (AcHNL, which was able to catalyse the (R-selective synthesis of mandelonitrile with significantly better conversion (97% and enantioselectivity (96.7% than other cupin HNLs.

  11. Discovery of a novel (R)-selective bacterial hydroxynitrile lyase from Acidobacterium capsulatum.

    Science.gov (United States)

    Wiedner, Romana; Gruber-Khadjawi, Mandana; Schwab, Helmut; Steiner, Kerstin

    2014-06-01

    Hydroxynitrile lyases (HNLs) are powerful carbon-carbon bond forming enzymes. The reverse of their natural reaction - the stereoselective addition of hydrogen cyanide (HCN) to carbonyls - yields chiral cyanohydrins, versatile building blocks for the pharmaceutical and chemical industry. Recently, bacterial HNLs have been discovered, which represent a completely new type: HNLs with a cupin fold. Due to various benefits of cupins (e.g. high yield recombinant expression in Escherichia coli), the class of cupin HNLs provides a new source for interesting, powerful hydroxynitrile lyases in the ongoing search for HNLs with improved activity, enantioselectivity, stability and substrate scope. In this study, database mining revealed a novel cupin HNL from Acidobacterium capsulatum ATCC 51196 (AcHNL), which was able to catalyse the (R)-selective synthesis of mandelonitrile with significantly better conversion (97%) and enantioselectivity (96.7%) than other cupin HNLs.

  12. The Anderson-Darling test of fit for the power law distribution from left censored samples

    CERN Document Server

    Coronel-Brizio, H F

    2010-01-01

    Maximum likelihood estimation and a test of fit based on the Anderson-Darling statistic is presented for the case of the power law distribution when the parameters are estimated from a left-censored sample. Expressions for the maximum likelihood estimators and tables of asymptotic percentage points for the A^2 statistic are given. The technique is illustrated for data from the Dow Jones Industrial Average index, an example of high theoretical and practical importance in Econophysics, Finance, Physics, Biology and, in general, in other related Sciences such as Complexity Sciences.

  13. Flavobacterium capsulatum

    African Journals Online (AJOL)

    organism was resistant or poorly sensitive to all antibiotics except carbenicillin. Cure was brought about by using maximal doses of this drug. The source of the infection could not be proved, but it dated from an orthopaedic procedure to the elbow which was followed by a chronic cellulitis at the operation site. Since this is an ...

  14. Media darling

    CERN Multimedia

    Chalmers, Matthew

    2008-01-01

    He is the media-friendly face of particle physics, appearing on countless TV and radio shows in the run-up to the opening of CERN's Large Hadron Collider. Matthew Chalmers discovers how Brian Cox finds the time to be both a physicist and a media personality. (2 pages)

  15. Fungemia por Histoplasma capsulatum em pacientes com a síndrome da imunodeficiência adquirida: detecção através da técnica de hemocultivo por lise-centrifugação

    OpenAIRE

    Flávio de Mattos Oliveira; Sérgio Sônego Fernandes; Cecília Bittencourt Severo; Luciana Silva Guazzelli; Luiz Carlos Severo

    2007-01-01

    Progressive disseminated histoplasmosis (PDH) is an increasingly common cause of infection in patients with acquired immune deficiency syndrome (AIDS). We report 21 cases of PDH associated with AIDS diagnosed by lysis-centrifugation blood culture method. The most prevalent clinical findings were fever, weight loss, respiratory symptoms, and mucocutaneous lesions. Chest roentgenogram showed diffuse pulmonary infiltrates in 13 of 21 patients (62%). Brochoalveolar fluid has yelded positive cultu...

  16. Integrative Governance of Environmental Water in Australia's Murray-Darling Basin: Evolving Challenges and Emerging Pathways.

    Science.gov (United States)

    Bischoff-Mattson, Zachary; Lynch, Amanda H

    2017-07-01

    Integration, a widely promoted response to the multi-scale complexities of social-environmental sustainability, is diversely and sometimes poorly conceptualized. In this paper we explore integrative governance, which we define as an iterative and contextual process for negotiating and advancing the common interest. We ground this definition in a discussion of institutional factors conditioning integrative governance of environmental water in Australia's Murray-Darling Basin. The Murray-Darling Basin is an iconic system of social-ecological complexity, evocative of large-scale conservation challenges in other developed arid river basins. Our critical assessment of integrative governance practices in that context emerges through analysis of interviews with policy participants and documents pertaining to environmental water management in the tri-state area of southwestern New South Wales, northwestern Victoria, and the South Australian Riverland. We identify four linked challenges: (i) decision support for developing socially robust environmental water management goals, (ii) resource constraints on adaptive practice, (iii) inter-state differences in participatory decision-making and devolution of authority, and (iv) representative inclusion in decision-making. Our appraisal demonstrates these as pivotal challenges for integrative governance in the common interest. We conclude by offering a perspective on the potential for supporting integrative governance through the bridging capacity of Australia's Commonwealth Environmental Water Holder.

  17. Reviewing the adoption and impact of water markets in the Murray-Darling Basin, Australia

    Science.gov (United States)

    Wheeler, S.; Loch, A.; Zuo, A.; Bjornlund, H.

    2014-10-01

    Water markets have increasingly been adopted as a reallocation tool around the world as water scarcity intensifies. Water markets were first introduced in Australia in the 1980s, and water entitlement and allocation trade have been increasingly adopted by both private individuals and governments. As well as providing an overview of water policy in Australia since the 1900s, this paper examines the adoption of water trading in the southern Murray-Darling Basin of Australia (the largest hydrologically connected water market in Australia), and investigates the associated social, economic and environmental impacts that have arisen from the implementation of water markets. This study found that up to 86% of irrigators in one state in the southern Murray-Darling Basin had undertaken at least one water market trade by 2010-2011, hence, water market strategies are now a common tool employed by irrigators to assist their farm management. A variety of institutional, policy and informational changes are identified to increase the benefits from water markets in the future. There is no doubt that managing the impact of climate change and water scarcity are intertwined, suggesting that policy, institutional and governance responses should be similarly structured and coordinated.

  18. A rare case of primary mucocutaneous histoplasmosis presenting as ...

    African Journals Online (AJOL)

    healing and disfiguring upper lip ulcer. ... with complete resolution of the ulcer. Antiretroviral therapy was also initiated. Keywords: HIV, Histoplasmosis, Histoplasma Capsulatum, mucocutaneous, ulcer, immunocompromised, Itraconazole ...

  19. Education, Place and Sustainability: A Literature Review and Overview of Curriculum and Policy in the States and the Territory of the Murray-Darling Basin

    Science.gov (United States)

    Roberts, Philip; Downes, Natalie; Cook, Louise; Heiner, Irmgard; Caffery, Jo

    2014-01-01

    This report has been developed as part of the MDBfutures Collaborative Research Network project "Towards Place Based Education in the Murray-Darling Basin." The project explores the ways in which sustainability is understood in Murray Darling Basin (MDB) communities of Australia (including Indigenous, rural, small towns and regional…

  20. Darling Ingredients, Inc. (d/b/a Dar Pro), Sioux City, Iowa - Clean Water Act Public Notice

    Science.gov (United States)

    EPA is providing notice of a proposed Administrative Penalty Assessment against Darling Ingredients, Inc. for alleged violations of Section 311(j) of the CWA, 33 U.S.C. § 1321(j), and regulations promulgated thereunder at 40 C.F.R. Part 112 for the require

  1. Risk management frameworks: supporting the next generation of Murray-Darling Basin water sharing plans

    Directory of Open Access Journals (Sweden)

    G. M. Podger

    2014-09-01

    Full Text Available Water jurisdictions in Australia are required to prepare and implement water resource plans. In developing these plans the common goal is realising the best possible use of the water resources – maximising outcomes while minimising negative impacts. This requires managing the risks associated with assessing and balancing cultural, industrial, agricultural, social and environmental demands for water within a competitive and resource-limited environment. Recognising this, conformance to international risk management principles (ISO 31000:2009 have been embedded within the Murray-Darling Basin Plan. Yet, to date, there has been little strategic investment by water jurisdictions in bridging the gap between principle and practice. The ISO 31000 principles and the risk management framework that embodies them align well with an adaptive management paradigm within which to conduct water resource planning. They also provide an integrative framework for the development of workflows that link risk analysis with risk evaluation and mitigation (adaptation scenarios, providing a transparent, repeatable and robust platform. This study, through a demonstration use case and a series of workflows, demonstrates to policy makers how these principles can be used to support the development of the next generation of water sharing plans in 2019. The workflows consider the uncertainty associated with climate and flow inputs, and model parameters on irrigation and hydropower production, meeting environmental flow objectives and recreational use of the water resource. The results provide insights to the risks associated with meeting a range of different objectives.

  2. Episodic recharge and climate change in the Murray-Darling Basin, Australia

    Science.gov (United States)

    Crosbie, Russell S.; McCallum, James L.; Walker, Glen R.; Chiew, Francis H. S.

    2012-03-01

    In semi-arid areas, episodic recharge can form a significant part of overall recharge, dependant upon infrequent rainfall events. With climate change projections suggesting changes in future rainfall magnitude and intensity, groundwater recharge in semi-arid areas is likely to be affected disproportionately by climate change. This study sought to investigate projected changes in episodic recharge in arid areas of the Murray-Darling Basin, Australia, using three global warming scenarios from 15 different global climate models (GCMs) for a 2030 climate. Two metrics were used to investigate episodic recharge: at the annual scale the coefficient of variation was used, and at the daily scale the proportion of recharge in the highest 1% of daily recharge. The metrics were proportional to each other but were inconclusive as to whether episodic recharge was to increase or decrease in this environment; this is not a surprising result considering the spread in recharge projections from the 45 scenarios. The results showed that the change in the low probability of exceedance rainfall events was a better predictor of the change in total recharge than the change in total rainfall, which has implications for the selection of GCMs used in impact studies and the way GCM results are downscaled.

  3. Risk management frameworks: supporting the next generation of Murray-Darling Basin water sharing plans

    Science.gov (United States)

    Podger, G. M.; Cuddy, S. M.; Peeters, L.; Smith, T.; Bark, R. H.; Black, D. C.; Wallbrink, P.

    2014-09-01

    Water jurisdictions in Australia are required to prepare and implement water resource plans. In developing these plans the common goal is realising the best possible use of the water resources - maximising outcomes while minimising negative impacts. This requires managing the risks associated with assessing and balancing cultural, industrial, agricultural, social and environmental demands for water within a competitive and resource-limited environment. Recognising this, conformance to international risk management principles (ISO 31000:2009) have been embedded within the Murray-Darling Basin Plan. Yet, to date, there has been little strategic investment by water jurisdictions in bridging the gap between principle and practice. The ISO 31000 principles and the risk management framework that embodies them align well with an adaptive management paradigm within which to conduct water resource planning. They also provide an integrative framework for the development of workflows that link risk analysis with risk evaluation and mitigation (adaptation) scenarios, providing a transparent, repeatable and robust platform. This study, through a demonstration use case and a series of workflows, demonstrates to policy makers how these principles can be used to support the development of the next generation of water sharing plans in 2019. The workflows consider the uncertainty associated with climate and flow inputs, and model parameters on irrigation and hydropower production, meeting environmental flow objectives and recreational use of the water resource. The results provide insights to the risks associated with meeting a range of different objectives.

  4. The evolution and performance of river basin management in the Murray-Darling Basin

    Directory of Open Access Journals (Sweden)

    Andrew Ross

    2016-09-01

    Full Text Available We explore bioregional management in the Murray-Darling Basin (MDB in Australia through the institutional design characteristics of the MDB River Basin Organization (RBO, the actors and organizations who supported and resisted the establishment of the RBO, and the effectiveness of the RBO. During the last 25 years, there has been a major structural reform in the MDB RBO, which has changed from an interstate coordinating body to an Australian government agency. Responsibility for basin management has been centralized under the leadership of the Australian government, and a comprehensive integrated Basin plan has been adopted. The driving forces for this centralization include national policy to restore river basins to sustainable levels of extraction, state government difficulties in reversing overallocation of water entitlements, the millennium drought and its effects, political expediency on the part of the Australian government and state governments, and a major injection of Australian government funding. The increasing hierarchy and centralization of the MDB RBO does not follow a general trend toward multilevel participative governance of RBOs, but decentralization should not be overstated because of the special circumstances at the time of the centralization and the continuing existence of some decentralized elements, such as catchment water plans, land use planning, and water quality. Further swings in the centralization-decentralization pendulum could occur. The MDB reform has succeeded in rebalancing Basin water allocations, including an allocation for the environment and reduced diversion limits. There are some longer term risks to the implementation of reform, including lack of cooperation by state governments, vertical coordination difficulties, and perceived reductions in the accountability and legitimacy of reform at the local level. If implementation of the Basin plan is diverted or delayed, a new institution, the Commonwealth

  5. A review of historic and future hydrological changes in the Murray-Darling Basin

    Science.gov (United States)

    Leblanc, Marc; Tweed, Sarah; Van Dijk, Albert; Timbal, Bertrand

    2012-01-01

    The Murray-Darling Basin is Australia's food bowl and home to many iconic water bodies that are culturally and ecologically highly valued. The recent Millennium Drought (from mid-1990s to 2009) was the most severe hydrological drought since records started in the late 19th century. It severely impacted on the basin and for many acted as a wake-up call. To address the ongoing declines in water resources and environmental conditions and to prepare the region for climate change, Australia's Governments are currently attempting to introduce a new comprehensive, and integrated approach to the management of the basin's water resources. In this paper, long-term time series of climate, hydrological and environmental data are used to analyze how compounding stresses have gradually affected the hydrological system and its services. Major hydroclimatic stresses considered in this paper include salinity, water use, droughts, and climate change. Other, more localized or minor stresses exist (groundwater extraction, farm dams, afforestation, bush fires, cyanobacterial blooms and pollutants) and are reviewed more briefly. The history of water policy and planning shows that Government actions have been strongly influential on the basin. A shift in the strategic goals from water development to the protection and restoration of environmental assets is noticeable since the mid 1990s. Median climate change projections by 2030 indicate smaller reductions in rainfall and runoff than those observed during the recent Millennium Drought, but have a relatively high uncertainty attached to them. The use of regional approaches to reduce that uncertainty, such as statistical downscaling, points to a sizeable decline in rainfall by the end of the century. Most climate projections used for planning consider greenhouse emission scenarios that have smaller global emission trends than the one observed over the last decade. Other, 'less optimistic' scenarios have to be considered for long-term water

  6. Mapping the spatial distribution of subsurface saline material in the Darling River valley

    Science.gov (United States)

    Triantafilis, John; Buchanan, Sam Mostyn

    2010-02-01

    In the Australian landscape larg stores of soluble salt are present naturally. In many cases it is attributable to salts entrapped as marine sediment in earlier geological time. At the district level, the need for information on the presence of saline subsurface material is increasing, particularly for its application to salinity hazard assessment and environmental management. This is the case in irrigated areas, where changes in hydrology can result in secondary salinisation. To reduce the expense, environmental studies use a regression relationship to make use of more readily observed measurements (e.g. electromagnetic (EM) data) which are strongly correlated with the variable of interest. In this investigation a methodology is outlined for mapping the spatial distribution of average subsurface (6-12 m) salinity (EC e — mS m - 1 ) using an environmental correlation with EM34 survey data collected across the Bourke Irrigation District (BID) in the Darling River valley. The EM34 is used in the horizontal dipole mode at coil configurations of 10 (EM34-10), 20 (EM34-20), and 40 (EM34-40). A multiple-linear regression (MLR) relationship is established between average subsurface EC e and the three EM34 signal data using a forward modeling stepwise linear modeling approach. The spatial distribution of average subsurface salinity generally reflects the known surface expression of point-source salinisation and provides information for future environmental monitoring and natural resource management. The generation of EM34 data on various contrived grids (i.e. 1, 1.5, 2. 2.5 and 3 km) indicates that in terms of accuracy, the data available on the 0.5 (RMSE = 188) and 1 km (RMSE = 283) grid are best, with the least biased predictions achieved using 1 (ME = - 1) and 2 km (ME = 12) grids. Viewing the spatial distribution of subsurface saline material showed that the 0.5 km spacing is optimal, particularly in order to account for short-range spatial variation between various

  7. Effects of sediment quality on macroinvertebrates in the Sunraysia region of the Murray-Darling Rivers, Australia

    Energy Technology Data Exchange (ETDEWEB)

    Sharley, David J. [Centre for Environmental Stress and Adaptation Research-Hoffmann Laboratory, Zoology Department, University of Melbourne, Level 2, Bio 21 Institute, 30 Flemington Road, Parkville, Victoria 3010 (Australia)], E-mail: sharleyd@unimelb.edu.au; Hoffmann, Ary A. [Centre for Environmental Stress and Adaptation Research-Hoffmann Laboratory, Zoology Department, University of Melbourne, Level 2, Bio 21 Institute, 30 Flemington Road, Parkville, Victoria 3010 (Australia); Pettigrove, Vincent [Research and Technology, Melbourne Water, PO Box 4342, Melbourne, Victoria 3001 (Australia)

    2008-12-15

    A field-based microcosm approach was tested to identify deterioration of sediment quality in waterways using freshwater macroinvertebrates. The method can potentially identify the nature of contaminants based on species-specific responses. Sediments were collected from the Murray and Darling Rivers and irrigation drains within the Sunraysia region of south-eastern Australia and compared to non-polluted reference sediment. Clean sediments were also spiked with fertiliser to test whether nutrients affected the aquatic fauna. Seven of the eight sediments from the Sunraysia region had a negative impact on the macroinvertebrates, in particular sediment from the Darling River, which supported an impoverished fauna. Three species of chironomid showed varied responses to sediment quality and, although it was hypothesised that nutrients may have impacted on the macroinvertebrate fauna, the results suggest that other pollutants are also involved. The field-based microcosm method proved effective for determining the impact of sediment quality on indigenous macroinvertebrates. - Sediment quality effects on freshwater macroinvertebrates are isolated.

  8. Prioritizing Wetlands for Waterbirds in a Boom and Bust System: Waterbird Refugia and Breeding in the Murray-Darling Basin.

    Directory of Open Access Journals (Sweden)

    Gilad Bino

    Full Text Available Dryland rivers have considerable flow variability, producing complex ecosystems, processes, and communities of organisms that vary over space and time. They are also among the more vulnerable of the world's ecosystems. A key strategy for conservation of dryland rivers is identifying and maintaining key sites for biodiversity conservation, particularly protecting the quantity and quality of flow and flooding regimes. Extreme variability considerably challenges freshwater conservation planning. We systematically prioritised wetlands for waterbirds (simultaneously for 52 species, across about 13.5% of the Murray-Darling Basin (1,061,469 km2, using a 30-year record of systematic aerial surveys of waterbird populations. Nine key wetlands in this area, primarily lakes, floodplains, and swamps, consistently contributed to a representation target (80% of total abundances of all 52 waterbird species. The long temporal span of our data included dramatic availability (i.e., booms and scarcity (i.e., busts of water, providing a unique opportunity to test prioritisation at extremes of variation. These extremes represented periods when waterbirds were breeding or concentrating on refugia, varying wetland prioritisation. In dry years, important wetlands for waterbirds were riverine and lacustrine (12 wetlands but this changed in wet years to lacustrine and palustrine (8 wetlands. Such variation in ecosystem condition substantially changes the relative importance of individual wetlands for waterbirds during boom and bust phases. Incorporating this variability is necessary for effective conservation of Murray-Darling Basin waterbirds, with considerable generality for other similarly variable systems around the world.

  9. Prioritizing Wetlands for Waterbirds in a Boom and Bust System: Waterbird Refugia and Breeding in the Murray-Darling Basin.

    Science.gov (United States)

    Bino, Gilad; Kingsford, Richard T; Porter, John

    2015-01-01

    Dryland rivers have considerable flow variability, producing complex ecosystems, processes, and communities of organisms that vary over space and time. They are also among the more vulnerable of the world's ecosystems. A key strategy for conservation of dryland rivers is identifying and maintaining key sites for biodiversity conservation, particularly protecting the quantity and quality of flow and flooding regimes. Extreme variability considerably challenges freshwater conservation planning. We systematically prioritised wetlands for waterbirds (simultaneously for 52 species), across about 13.5% of the Murray-Darling Basin (1,061,469 km2), using a 30-year record of systematic aerial surveys of waterbird populations. Nine key wetlands in this area, primarily lakes, floodplains, and swamps, consistently contributed to a representation target (80%) of total abundances of all 52 waterbird species. The long temporal span of our data included dramatic availability (i.e., booms) and scarcity (i.e., busts) of water, providing a unique opportunity to test prioritisation at extremes of variation. These extremes represented periods when waterbirds were breeding or concentrating on refugia, varying wetland prioritisation. In dry years, important wetlands for waterbirds were riverine and lacustrine (12 wetlands) but this changed in wet years to lacustrine and palustrine (8 wetlands). Such variation in ecosystem condition substantially changes the relative importance of individual wetlands for waterbirds during boom and bust phases. Incorporating this variability is necessary for effective conservation of Murray-Darling Basin waterbirds, with considerable generality for other similarly variable systems around the world.

  10. Delivery of marine chloride in precipitation and removal by rivers in the Murray-Darling Basin, Australia

    Science.gov (United States)

    Simpson, H. James; Herczeg, Andrew L.

    1994-02-01

    Rising groundwater tables and increasing river salinities are major problems in the semi-arid Murray-Darling (M-D) Drainage Basin of SE Australia. Chemical data from precipitation, rivers and groundwaters are integrated here to estimate chloride fluxes in the context of dramatic alteration of the hydrologic balance in the basin initiated during European settlement approximately 150 years ago. Our analysis indicates that about half of Cl deposition from the atmosphere in the western Murray Basin is derived from resuspended regional continental dust, resulting in high Ca/Na ratios in bulk precipitation samples. Estimates of recent marine deposition of Cl to the M-D Basin were made by excluding rainfall data from sites having high Ca/Na ratios. Basin-wide budgets for Cl delivery from the atmosphere and removal by rivers indicate that the Darling Basin retains about 90% of current annual input of marine Cl whereas the River Murray exports two to three times current annual input to the Murray Basin (exclusive of the Darling). Some implications from this study are as follows: (1) Re-evaluation of net marine Cl input by rain implies that recent model calculations of {36Cl}/{Cl} inputs from the atmosphere to the Murray Basin may differ significantly from actual values. In particular, net {36Cl}/{Cl} input ratios from the atmosphere to the western Murray Basin may be a factor of two higher than those derived assuming all Cl in rain is of recent marine origin. (2) The very large inventories of salt within the two catchments, especially the saline groundwaters of the Murray Basin, accumulated over very long periods, probably of the order of a few million years. The current rearrangements of stored salinity as a result, at least in part, of human perturbations of the hydrologic balance are likely to continue for an extended period because of the massive quantities of salts involved, and thus present major limitations on options for long-term management of the M-D Basin.

  11. Why Henry Giroux's Democratic Pedagogy Is Crucial for Confronting Failed Corporate School Reform and How Liberals Like Ravitch and Darling-Hammond Are Making Things Worse

    Science.gov (United States)

    Saltman, Kenneth J.

    2012-01-01

    Progressive media and the academic community outside of education has largely embraced liberal criticisms of corporate school reform or neoliberal educational restructuring, typified by the highly publicized writing and speaking of Diane Ravitch and Linda Darling-Hammond. Despite offering valuable policy information, the liberal view is grounded…

  12. Predictability of current and future multi-river discharges: Ganges, Brahmaputra, Yangtze, Blue Nile, and Murray-Darling rivers

    Science.gov (United States)

    Jian, Jun

    2007-12-01

    Determining river discharge is of critical importance to many societies as they struggle with fresh water supply and risk of flooding. In Bangladesh, floods occur almost every year but with sufficient irregularity to have adverse social and economical consequences. Important goals are to predict the discharge to be used for the optimization of agricultural practices, disaster mitigation and water resource management. The aim of this study is to determine the predictability of river discharge in a number of major rivers on time scale varying from weeks to a century. We investigated predictability considering relationship between SST and discharge. Next, we consider IPCC model projections of river discharge while the models are statistically adjusted against observed discharges. In this study, we consider five rivers, the Ganges, the Brahmaputra, the Yangtze, the Blue Nile, and the Murray-Darling Rivers. On seasonal time scales, statistically significant correlations are found between mean monthly equatorial Pacific sea surface temperature (SST) and the summer Ganges discharge with lead times of 2-3 months due to oscillations of the El Nino-Southern Oscillation (ENSO) phenomena. In addition, there are strong correlations in the southwest and northeast Pacific. These, too, appear to be tied to the ENSO cycle. The Brahmaputra discharge, on the other hand, shows somewhat weaker relationships with tropical SST. Strong lagged correlations relationships are found with SST in the Bay of Bengal but these are the result of very warm SSTs and exceptional Brahmaputra discharge during the summer of 1998. When this year is removed from the time series, relationships weaken everywhere except in the northwestern Pacific for the June discharge and in areas of the central Pacific straddling the equator for the July discharge. The relationships are relative strong, but they are persistent from month to month and suggest that two different and sequential factors influence Brahmaputra

  13. A Farm-level Decision Support Tool for Irrigation Infrastructure Investment Decisions: Application in Murray Darling Basin of Australia

    Science.gov (United States)

    Chen, C.; Hafeez, M.; Mushtaq, S.

    2008-12-01

    Increasing water scarcity, climate change and pressure to provide water for environmental flows urge irrigators to be more efficient in water scarce Murray Darling Basin catchment. In Australia, ongoing water reforms and most recent National Water Security Plan offer incentives to irrigators to adjust their farming practices by adopting water saving irrigation infrastructures to match with soils, crop and climatic conditions. WaterWorks is a decision support tool to facilitate irrigators to make long and short term irrigation infrastructure investment decision at the farm level. It assists irrigators to improve the economic efficiency, water use efficiency and environmental performance of their farm businesses. WaterWorks has been tested, validated and accepted by the irrigation community and researchers in Southern Part of Murray Darling Basin. The interface of WaterWorks is user-friendly and flexible. The simulation and optimisation module in WaterWorks provides an opportunity to evaluate infrastructure investment decisions to suit their seasonal or long-term water availability. The sensitivity analysis allows substantiating the impact of major variables. Net present value, internal rate of return, benefit cost ratio and payback period are used to analyse the costs and benefits of modern irrigation technology. Application of WaterWorks using a whole farm-level case study indicates its effectiveness in making long term and short term investment decisions. WaterWorks can be easily integrated into commercial software such as spreadsheets, GIS, real time data acquisition and control systems to further enhance its usability. WaterWorks can also be used in regional development planning.

  14. Firewood harvest from forests of the Murray-Darling Basin, Australia. Part 2: Plantation resource required to supply present demand

    Energy Technology Data Exchange (ETDEWEB)

    West, P.W. [School of Environmental Science and Management, Southern Cross University, Lismore, NSW 2480 (Australia); SciWest Consulting, 16 Windsor Court, Goonellabah, NSW 2480 (Australia); Cawsey, E.M.; Stol, J. [CSIRO Sustainable Ecosystems, GPO Box 284, Canberra, ACT 2601 (Australia); Freudenberger, D. [Greening Australia, PO Box 74, Yarralumla, ACT 2600 (Australia)

    2008-12-15

    The Murray-Darling Basin covers 1 M km{sup 2} and occupies most of inland, south-eastern, mainland Australia. Large areas have been cleared and are now used for agriculture. In this paper, estimates are made of the minimum area of Eucalyptus globulus plantation forests needed to be established in the Basin to supply 2.25 M oven-dry t yr{sup -1} of firewood annually, the amount of firewood harvested presently from the native forests which remain in the Basin. If plantations were established in higher rainfall areas along the eastern and southern boundaries of the Basin, it was estimated that a minimum of just over 200,000 ha of plantations would be required, grown on a 10-yr rotation. If plantations were restricted to less productive areas of lower rainfall (<900 mm yr{sup -1}), or to areas where land clearing for agriculture has been particularly intensive, a minimum of just under 350,000 ha would be required, grown on an 11-yr rotation. If planting was restricted to soils in the Basin at high risk of salinisation from agriculture, which are generally in areas of lower rainfall, a minimum of about 600,000 ha would be required, grown on a 20-yr rotation. It is considered that the practicalities of plantation establishment in the Basin would require appreciably larger areas of plantations than these minima. (author)

  15. Dynamics of Murray-Darling floodplain forests under multiple stressors: The past, present, and future of an Australian icon

    Science.gov (United States)

    Mac Nally, Ralph; Cunningham, Shaun C.; Baker, Patrick J.; Horner, Gillis J.; Thomson, James R.

    2011-12-01

    We review the human actions, proximal stressors and ecological responses for floodplain forests Australia's largest river system—the Murray-Darling Basin. A conceptual model for the floodplain forests was built from extensive published information and some unpublished results for the system, which should provide a basis for understanding, studying and managing the ecology of floodplains that face similar environmental stresses. Since European settlement, lowlands areas of the basin have been extensively cleared for agriculture and remnant forests heavily harvested for timber. The most significant human intervention is modification of river flows, and the reduction in frequency, duration and timing of flooding, which are compounded by climate change (higher temperatures and reduced rainfall) and deteriorating groundwater conditions (depth and salinity). This has created unfavorable conditions for all life-history stages of the dominant floodplain tree (Eucalyptus camaldulensis Dehnh.). Lack of extensive flooding has led to widespread dieback across the Murray River floodplain (currently 79% by area). Management for timber resources has altered the structure of these forests from one dominated by large, widely spreading trees to mixed-aged stands of smaller pole trees. Reductions in numbers of birds and other vertebrates followed the decline in habitat quality (hollow-bearing trees, fallen timber). Restoration of these forests is dependent on substantial increases in the frequency and extent of flooding, improvements in groundwater conditions, re-establishing a diversity of forest structures, removal of grazing and consideration of these interacting stressors.

  16. Building resilient pathways to transformation when "no one is in charge": insights from Australia's Murray-Darling Basin

    Directory of Open Access Journals (Sweden)

    Nick Abel

    2016-06-01

    Full Text Available Climate change and its interactions with complex socioeconomic dynamics dictate the need for decision makers to move from incremental adaptation toward transformation as societies try to cope with unprecedented and uncertain change. Developing pathways toward transformation is especially difficult in regions with multiple contested resource uses and rights, with diverse decision makers and rules, and where high uncertainty is generated by differences in stakeholders' values, understanding of climate change, and ways of adapting. Such a region is the Murray-Darling Basin, Australia, from which we provide insights for developing a process to address these constraints. We present criteria for sequencing actions along adaptation pathways: feasibility of the action within the current decision context, its facilitation of other actions, its role in averting exceedance of a critical threshold, its robustness and resilience under diverse and unexpected shocks, its effect on future options, its lead time, and its effects on equity and social cohesion. These criteria could potentially enable development of multiple stakeholder-specific adaptation pathways through a regional collective action process. The actual implementation of these multiple adaptation pathways will be highly uncertain and politically difficult because of fixity of resource-use rights, unequal distribution of power, value conflicts, and the likely redistribution of benefits and costs. We propose that the approach we outline for building resilient pathways to transformation is a flexible and credible way of negotiating these challenges.

  17. 75 Years of Histoplasmosis Outbreaks in the United States

    Centers for Disease Control (CDC) Podcasts

    2016-03-24

    Kaitlin Benedict, CDC epidemiologist, discusses Histoplasma capsulatum infection.  Created: 3/24/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 3/24/2016.

  18. The Murrumbidgee Monitoring Network: Supporting CEOP, GEWEX and Hydrological Research in the Murray-Darling Basin, Australia

    Science.gov (United States)

    Ellett, K. M.; Western, A. W.; Walker, J. P.; Sirawardena, L.; Young, R. I.; Smith, A. B.; Flint, A. L.; Summerell, G.

    2006-12-01

    In 2001 a network of 18 soil moisture monitoring sites were installed across the 80,000 square km Murrumbidgee River catchment in Australia with the aim of evaluating the land surface component of the Australian Bureau of Meteorology's operational weather forecasting model. Since that time the Murrumbidgee Monitoring Network (MMN) has evolved to include 46 sites for continuous measurement of root-zone soil moisture, soil temperature and precipitation, as well as observations of deep soil moisture and ground water variability. Much of these data will soon be incorporated into the World Climate Research Programme's Coordinated Enhanced Observing Period (CEOP) database (www.ceop.net) marking a substantial new contribution from the Australian continent. This paper provides an overview of the MMN and presents current results from applications in a number of regional-scale research projects including the Murray-Darling Basin GEWEX study and the National Airborne Field Experiment 2006 (NAFE'06) aimed at improving the retrieval of soil moisture and vegetation parameters from airborne and satellite platforms. The MMN also plays an integral role in the HYDROGRACE project with the objectives of (1) providing the first in-situ based validation of terrestrial water storage observations from the GRACE (Gravity Recovery and Climate Experiment) mission and (2) improving regional-scale model simulations through data assimilation of GRACE observations. The MMN is part of the broader OzNet hydrological monitoring network throughout eastern Australia. Details on OzNet and the projects mentioned above are provided at www.oznet.unimelb.edu.au.

  19. A comparative study of historical droughts over Texas, USA and Murray-Darling Basin, Australia: Factors influencing initialization and cessation

    Science.gov (United States)

    Verdon-Kidd, Danielle C.; Scanlon, Bridget R.; Ren, Tong; Fernando, D. Nelun

    2017-02-01

    Water availability and food security are tightly coupled on a global scale. The occurrence of drought puts this balance at risk due to reductions in dryland farming production and water allocations to irrigated agriculture. Improved understanding of drought initiation and cessation would therefore be beneficial for drought planning and management. The study objective was to determine factors affecting drought initiation and cessation over the past century in two climatologically similar regions that represent net agricultural exporters; south central U.S. (Texas) and southeast Australia (Murray Darling Basin, MDB). Drought indices included the Standardized Precipitation Index (SPI, 1900-2014) for meteorological drought, the Normalized Difference Vegetation Index (NDVI, 1981-2014) for agricultural drought, and the Streamflow Drought Index (SDI, 1930-2014) for hydrological drought. Results show that meteorological drought tends to develop gradually over a period of up to six months, with agricultural drought developing shortly thereafter (within one month) in both regions. Evidence of hydrological drought was observed within one month (Texas) and within four months (MDB) on average after meteorological drought was established. Further, droughts appear to cease more quickly than they initiate over Texas, whereas rates of drought initiation and cessation are similar over the MDB. Drought breaking rainfall in Texas is generally a result of a southward shift in the Polar Jet Stream or a low-pressure trough over central North America, whereas drought cessation in the MDB is typically associated with a monsoon trough or low-pressure system in the Tasman Sea/Great Australian Bight. Improved knowledge of the climate mechanisms controlling the onset and termination of drought periods should enhance drought forecasts and improve drought management practices, particularly in regions where water security is a primary objective.

  20. Disseminated histoplasmosis in a Bengal tiger (Panthera tigris).

    Science.gov (United States)

    Keller, Dominique L; Steinberg, Howard; Sladky, Kurt K

    2011-12-01

    Disseminated infection with Histoplasma capsulatum was diagnosed in a 7-yr-old female Bengal tiger (Panthera tigris). Clinical signs were nonspecific with the exception of brief periods of tachypnea for 5 days prior to death. H. capsulatum organisms were found in the lungs, tracheobronchial lymph nodes, and liver. Diagnosis was confirmed by tracheal wash, urine H. capsulatum enzyme immunoassay, and necropsy results. This report represents the first published account of disseminated histoplasmosis in a tiger.

  1. Toward long-lead operational forecasts of drought: An experimental study in the Murray-Darling River Basin

    Science.gov (United States)

    Barros, Ana P.; Bowden, Gavin J.

    2008-08-01

    SummaryResiliency and effectiveness in water resources management of drought is strongly depend on advanced knowledge of drought onset, duration and severity. The motivation of this work is to extend the lead time of operational drought forecasts. The research strategy is to explore the predictability of drought severity from space-time varying indices of large-scale climate phenomena relevant to regional hydrometeorology (e.g. ENSO) by integrating linear and non-linear statistical data models, specifically self-organizing maps (SOM) and multivariate linear regression analysis. The methodology is demonstrated through the step-by-step development of a model to forecast monthly spatial patterns of the standard precipitation index (SPI) within the Murray-Darling Basin (MDB) in Australia up to 12 months in advance. First, the rationale for the physical hypothesis and the exploratory data analysis including principal components, wavelet and partial mutual information analysis to identify and select predictor variables are presented. The focus is on spatial datasets of precipitation, sea surface temperature anomaly (SSTA) patterns over the Indian and Pacific Oceans, temporal and spatial gradients of outgoing longwave radiation (OLR) in the Pacific Ocean, and the far western Pacific wind-stress anomaly. Second, the process of model construction, calibration and evaluation is described. The experimental forecasts show that there is ample opportunity to increase the lead time of drought forecasts for decision support using parsimonious data models that capture the governing climate processes at regional scale. OLR gradients proved to be dispensable predictors, whereas SPI-based predictors appear to control predictability when the SSTA in the region [87.5°N-87.5°S; 27.5°E-67.5°W] and eastward wind-stress anomalies in the region [4°N-4°S; 130°E-160°E) are small, respectively, ±1° and ±0.01 dyne/cm 2, that is when ENSO activity is weak. The areal averaged 12-month

  2. Histoplasmosis en América Latina

    OpenAIRE

    Ricardo Negroni

    2011-01-01

    La histoplasmosis clásica es una micosis sistémica endémica, producida por el hongo dimorfo Histoplasma capsulatum var. capsulatum. La forma de micelios del agente causal se encuentra en la tierra y puede obtenerse en cultivos incubados a 28 °C, en tanto que su forma de levadura se encuentra en los tejidos animales parasitados, en general, en el interior de células del sistema monocítico-histiocitario y en los cultivos enriquecidos, cultivados a 37 °C. Histoplasma capsulatum presenta una fase...

  3. A Trans-disciplinary Hydrogeological Systems Analysis Approach for Identifying and Assessing Managed Aquifer Recharge Options: Example from the Darling River Floodplain, N.S.W., Australia

    Science.gov (United States)

    Lawrie, K.; Brodie, R. S.; Tan, K. P.; Halas, L.; Magee, J.; Gow, L.; Christensen, N. B.

    2013-12-01

    Surface water availability and quality generally limits managed aquifer recharge (MAR) opportunities in inland Australia's highly salinized landscapes and groundwater systems. Economic factors also commonly limit MAR investigations to shallow freshwater groundwater systems near existing infrastructure. Aquifer opportunities lie mainly in zones of fresh groundwater in relatively thin fluvial sedimentary aquifer systems with highly variable hydraulic properties. As part of a broader strategy to identify water savings in the Murray-Darling Basin, the Broken Hill Managed Aquifer Recharge (BHMAR) project was tasked with identifying and assessing MAR and/or groundwater extraction options to reduce evaporative losses from existing surface water storages, secure Broken Hill's water supply, protect the local environment and heritage, and return water to the river system. A trans-disciplinary research approach was used to identify and assess MAR options across a broad area of the Darling River floodplain. This methodology enabled the team to recognise fundamental problems in discipline approaches, helped identify critical data gaps, led to significant innovation across discipline boundaries, was critical in the development of a new hydrogeological conceptual model, facilitated development of new models of landscape, geological and tectonic evolution of the study area, and enabled completion of pre-commissioning maximal and residual MAR risk assessments. An airborne electromagnetics (AEM) survey, acquired over a large (>7,500 sq km) area of the Darling Floodplain, enabled rapid identification of a multi-layer sequence of aquifers and aquitards, while a phased assessment methodology was developed to rapidly identify and assess over 30 potential MAR targets (largely in fresh groundwater zones within palaeochannels and at palaeochannel confluences). Hydraulic properties were confirmed by a 7.5 km drilling program (100 sonic and rotary mud holes), and complementary field

  4. Climate change and environmental water reallocation in the Murray-Darling Basin: Impacts on flows, diversions and economic returns to irrigation

    Science.gov (United States)

    Kirby, J. M.; Connor, J.; Ahmad, M. D.; Gao, L.; Mainuddin, M.

    2014-10-01

    Increasing river environment degradation from historical growth in withdrawal is leading to reallocation of water from irrigation in many basins. We examine how potential reduction in irrigation allocations under a newly enacted environmental water plan for the Murray Darling Basin in Australia, in combination with projected climate change, impact on flows, diversions and the economic returns to irrigation. We use an integrated hydrology-economics model capable of simulating the year-to-year variability of flows, diversions, and economic returns to model three levels of reallocation (2400, 2750 and 3200 GL) under the historical climate, and under a dry, a median and a wet climate change projection. Previous assessments of the reallocation plan do not address climate change impacts, nor the impact of year to year variability in flows on economic returns. The broad results of this analysis are that estimated river flows and diversions are more sensitive to the range of climate change projections than to the range of diversion reallocation scenarios considered. The projected median climate change more or less removes from flows the gains to the environment resulting from reallocation. Reallocations only in combination with no climate change, or climate change at the wetter end of the range of projections, will lead to flows greater than those experienced under the water management regime prior to reallocation. The reduction in economic returns to irrigation is less than the reduction in water available for irrigation: a 25% reduction in the annual average water availability is estimated to reduce the annual average gross value of irrigated agricultural production by about 10%. This is consistent with expectation of economic theory (since more marginal activities are reduced first) and also with observations of reduced water availability and returns in the recent drought in the Murray-Darling Basin. Irrigation returns vary less across the range of climate change

  5. Trace-element systematics of sediments in the Murray-Darling Basin, Australia: Sediment provenance and palaeoclimate implications of fine scale chemical heterogeneity

    Energy Technology Data Exchange (ETDEWEB)

    Marx, Samuel K., E-mail: s.marx@uq.edu.au [Climate Research Group, School of Geography, Planning and Environmental Management, University of Queensland, St. Lucia, Brisbane, Qld 4072 (Australia); Kamber, Balz S. [Department of Earth Sciences, Laurentian University, Sudbury, Ontario, Canada P3E2C6 (Canada)

    2010-08-15

    A high-resolution dataset of trace element concentrations is presented for the Murray-Darling Basin, Australia, Australia's most important river system. The data were obtained by solution quadrupole ICP-MS resulting in concentrations for 44 elements. Of these, 21 were determined with a long-term external precision of better than 1% and a further 13 at a precision better than 2%. Trace element maps for the surface sediments constructed from such high precision data reveal small but coherent variations in the four major sub-catchments of the basin, even in ratios of elements with very similar geochemical behaviour, such as Y/Ho, Nb/Ta and Zr/Hf. The origin of these chemical fingerprints of drainage systems are discussed in terms of the geochemical character of the upper continental crust. The potential of trace element maps for palaeo-environmental and climatic reconstruction is then illustrated. First, a sample of dust collected in a trap located in the far southeastern corner of the study area is used to pinpoint the location of the dust source. Next the fine-scale change in down-stream alluvial sediment chemistry is analysed to estimate the importance of sediment contribution from tributaries with a view to reconstructing river flow dynamics. Finally, the chemistry of dune sediments is compared with surrounding floodplain alluvium to estimate relative age of deposition. These examples demonstrate that in low-elevation river systems, such as the Murray-Darling Basin, extended trace element maps of sediment offer substantially more applications than radiogenic isotope data alone.

  6. Input selection and performance optimization of ANN-based streamflow forecasts in the drought-prone Murray Darling Basin region using IIS and MODWT algorithm

    Science.gov (United States)

    Prasad, Ramendra; Deo, Ravinesh C.; Li, Yan; Maraseni, Tek

    2017-11-01

    Forecasting streamflow is vital for strategically planning, utilizing and redistributing water resources. In this paper, a wavelet-hybrid artificial neural network (ANN) model integrated with iterative input selection (IIS) algorithm (IIS-W-ANN) is evaluated for its statistical preciseness in forecasting monthly streamflow, and it is then benchmarked against M5 Tree model. To develop hybrid IIS-W-ANN model, a global predictor matrix is constructed for three local hydrological sites (Richmond, Gwydir, and Darling River) in Australia's agricultural (Murray-Darling) Basin. Model inputs comprised of statistically significant lagged combination of streamflow water level, are supplemented by meteorological data (i.e., precipitation, maximum and minimum temperature, mean solar radiation, vapor pressure and evaporation) as the potential model inputs. To establish robust forecasting models, iterative input selection (IIS) algorithm is applied to screen the best data from the predictor matrix and is integrated with the non-decimated maximum overlap discrete wavelet transform (MODWT) applied on the IIS-selected variables. This resolved the frequencies contained in predictor data while constructing a wavelet-hybrid (i.e., IIS-W-ANN and IIS-W-M5 Tree) model. Forecasting ability of IIS-W-ANN is evaluated via correlation coefficient (r), Willmott's Index (WI), Nash-Sutcliffe Efficiency (ENS), root-mean-square-error (RMSE), and mean absolute error (MAE), including the percentage RMSE and MAE. While ANN models are seen to outperform M5 Tree executed for all hydrological sites, the IIS variable selector was efficient in determining the appropriate predictors, as stipulated by the better performance of the IIS coupled (ANN and M5 Tree) models relative to the models without IIS. When IIS-coupled models are integrated with MODWT, the wavelet-hybrid IIS-W-ANN and IIS-W-M5 Tree are seen to attain significantly accurate performance relative to their standalone counterparts. Importantly

  7. Darling-Dennison resonance and Coriolis coupling in the bending overtones of the A 1A(u) state of acetylene, C2H2.

    Science.gov (United States)

    Merer, Anthony J; Yamakita, Nami; Tsuchiya, Soji; Steeves, Adam H; Bechtel, Hans A; Field, Robert W

    2008-08-07

    Rotational analyses have been carried out for the overtones of the nu(4) (torsion) and nu(6) (in-plane cis-bend) vibrations of the A (1)A(u) state of C(2)H(2). The v(4)+v(6)=2 vibrational polyad was observed in high-sensitivity one-photon laser-induced fluorescence spectra and the v(4)+v(6)=3 polyad was observed in IR-UV double resonance spectra via the ground state nu(3) (Sigma(+) (u)) and nu(3)+nu(4) (Pi(u)) vibrational levels. The structures of these polyads are dominated by the effects of vibrational angular momentum: Vibrational levels of different symmetry interact via strong a-and b-axis Coriolis coupling, while levels of the same symmetry interact via Darling-Dennison resonance, where the interaction parameter has the exceptionally large value K(4466)=-51.68 cm(-1). The K-structures of the polyads bear almost no resemblance to the normal asymmetric top patterns, and many local avoided crossings occur between close-lying levels with nominal K-values differing by one or more units. Least squares analysis shows that the coupling parameters change only slightly with vibrational excitation, which has allowed successful predictions of the structures of the higher polyads: A number of weak bands from the v(4)+v(6)=4 and 5 polyads have been identified unambiguously. The state discovered by Scherer et al. [J. Chem. Phys. 85, 6315 (1986)], which appears to interact with the K=1 levels of the 3(3) vibrational state at low J, is identified as the second highest of the five K=1 members of the v(4)+v(6)=4 polyad. After allowing for the Darling-Dennison resonance, the zero-order bending structure can be represented by omega(4)=764.71, omega(6)=772.50, x(44)=0.19, x(66)=-4.23, and x(46)=11.39 cm(-1). The parameters x(46) and K(4466) are both sums of contributions from the vibrational angular momentum and from the anharmonic force field. For x(46) these contributions are 14.12 and -2.73 cm(-1), respectively, while the corresponding values for K(4466) are -28.24 and -23

  8. Quantifying water requirements of riparian river red gum (Eucalyptus camaldulensis) in the Murray-Darling Basin, Australia: Implications for the management of environmental flows

    Science.gov (United States)

    Doody, Tanya M.; Colloff, Matthew J.; Davies, Micah; Koul, Vijay; Benyon, Richard G.; Nagler, Pamela L.

    2015-01-01

    Water resource development and drought have altered river flow regimes, increasing average flood return intervals across floodplains in the Murray-Darling Basin, Australia, causing health declines in riparian river red gum (Eucalyptus camaldulensis) forests and woodlands. Environmental flow allocations helped to alleviate water stress during the recent Millennium Drought (1997–2010), however, quantification of the flood frequency required to support healthy E. camaldulensis communities is still needed. We quantified water requirements of E. camaldulensis for two years across a flood gradient (trees inundated at frequencies of 1:2, 1:5 and 1:10 years) at Yanga National Park, New South Wales to help inform management decision-making and design of environmental flows. Sap flow, evaporative losses and soil moisture measurements were used to determine transpiration, evapotranspiration and plant-available soil water before and after flooding. A formula was developed using plant-available soil water post-flooding and average annual rainfall, to estimate maintenance time of soil water reserves in each flood frequency zone. Results indicated that soil water reserves could sustain 1:2 and 1:5 trees for 15 months and six years, respectively. Trees regulated their transpiration rates, allowing them to persist within their flood frequency zone, and showed reduction in active sapwood area and transpiration rates when flood frequencies exceeded 1:2 years. A leaf area index of 0.5 was identified as a potential threshold indicator of severe drought stress. Our results suggest environmental water managers may have greater flexibility to adaptively manage floodplains in order to sustain E. camaldulensis forests and woodlands than has been appreciated hitherto.

  9. Application of FISK, an invasiveness screening tool for non-native freshwater fishes, in the Murray-Darling Basin (southeastern Australia).

    Science.gov (United States)

    Vilizzi, Lorenzo; Copp, Gordon H

    2013-08-01

    The Fish Invasiveness Scoring Kit (FISK) is currently one of the most popular pre-screening tools for freshwater fishes. A recent upgrade has ensured its wider climatic relevance to countries with subtropical regions. This enhancement is of particular importance to Australia, which encompasses tropical, arid, and temperate zones, and where the introduction of non-native fish species poses a significant risk to biodiversity. In this study, 55 fish species previously evaluated in a U.K.-based calibration of FISK are reassessed for their potential invasiveness in the Murray-Darling Basin (MDB; southeastern Australia), the continent's largest catchment encompassing arid and temperate climates. Approximately half of the species were classed as "medium risk" and the other half as "high risk," and the ≥19 threshold previously identified from the calibration study was confirmed. The three highest scoring species (common carp Cyprinus carpio carpio, goldfish Carassius auratus, and eastern mosquitofish Gambusia holbrooki) were those already present and invasive in the area, whereas nearly half of the tropical and subtropical species had lower scores compared to U.K. assessments, possibly because of climate change predictions of drier conditions across the MDB. There were some discordances between FISK and two Australian-based assessment protocols, one of which is qualitative and the other represents a simplified version of FISK. Notably, the Australian origins of FISK should provide for an additional reason for further applications of the tool in other RA areas (i.e., drainage basins) of the continent, ultimately encouraging adoption as the country's reference screening tool for management and conservation purposes. © 2012 Society for Risk Analysis.

  10. Dating megafaunal extinction on the Pleistocene Darling Downs, eastern Australia: the promise and pitfalls of dating as a test of extinction hypotheses

    Science.gov (United States)

    Price, Gilbert J.; Webb, Gregory E.; Zhao, Jian-xin; Feng, Yue-xing; Murray, Andrew S.; Cooke, Bernard N.; Hocknull, Scott A.; Sobbe, Ian H.

    2011-04-01

    A key to understanding Late Pleistocene megafaunal extinction dynamics is knowledge of megafaunal ecological response(s) to long-term environmental perturbations. Strategically, that requires targeting fossil deposits that accumulated during glacial and interglacial intervals both before and after human arrival, with subsequent palaeoecological models underpinned by robust and reliable chronologies. Late Pleistocene vertebrate fossil localities from the Darling Downs, eastern Australia, provide stratigraphically-intact, abundant megafaunal sequences, which allows for testing of anthropogenic versus climate change megafauna extinction hypotheses. Each stratigraphic unit at site QML796, Kings Creek Catchment, was previously shown to have had similar sampling potential, and the basal units contain both small-sized taxa (e.g., land snails, frogs, bandicoots, rodents) and megafauna. Importantly, sequential faunal horizons show stepwise decrease in taxonomic diversity with the loss of some, but not all, megafauna in the geographically-small palaeocatchment. The purpose of this paper is to present the results of our intensive, multidisciplinary dating study of the deposits (>40 dates). Dating by means of accelerator mass spectrometry (AMS) 14C (targeting bone, freshwater molluscs, and charcoal) and thermal ionisation mass spectrometry U/Th (targeting teeth and freshwater molluscs) do not agree with each other and, in the case of AMS 14C dating, lack internal consistency. Scanning electron microscopy and rare earth element analyses demonstrate that the dated molluscs are diagenetically altered and contain aragonite cements that incorporated secondary young C, suggesting that such dates should be regarded as minimum ages. AMS 14C dated charcoals provide ages that occur out of stratigraphic order, and cluster in the upper chronological limits of the technique (˜40-48 ka). Again, we suggest that such results should be regarded as suspicious and only minimum ages. Subsequent

  11. Locally acquired disseminated histoplasmosis in a northern sea otter (Enhydra lutris kenyoni) in Alaska, USA.

    Science.gov (United States)

    Burek-Huntington, Kathy A; Gill, Verena; Bradway, Daniel S

    2014-04-01

    Histoplasmosis of local origin has not been reported in humans or wildlife in Alaska, and the disease has never been reported in a free-ranging marine mammal. In 2005 a northern sea otter (Enhydra lutris kenyoni) was found on Kodiak Island, Alaska, at 57° latitude north, far outside the known distribution of Histoplasma capsulatum. The animal died of disseminated histoplasmosis. Microorganisms consistent with Histoplasma sp. were observed on histopathology, and H. capsulatum was identified by PCR and sequencing. We suggest migratory seabirds or aerosol transmission through prevailing winds may have resulted in transmission to the sea otter.

  12. Spatial and temporal dynamic of flooding and vegetation response to flooding using remotely sensed data in the Murray -Darling Basin, Australia

    Science.gov (United States)

    Tulbure, Mirela; Kingsford, Richard; Broich, Mark

    2013-04-01

    Australia is the driest inhabited continent and river systems have highly variable flows in space and time. The Murray-Darling Basin (MDB), a catchment covering 14% of the continent contains the nation's largest rivers and important groundwater systems. The basin has highly variable rainfall patterns in space and time and is home to several wetlands of high hydrological and ecological value. However, variation in surface and ground water availability exacerbated by a long period of drought, combined with high water demands for irrigation, human use, and ecosystem health led to the need of managing water resources in an integrated fashion. Flushes of water, stored in dams, are being released during dry periods as environmental flows. Assessment of water resources and understanding of the effectiveness of environmental flows requires knowledge of long term trends in occurrence and extent of surface water and vegetation response to flooding and environmental flows. Satellite remote sensing is the only viable way for synoptically mapping and monitoring the extent and dynamic of flooding and vegetation response to flooding. Recent La Nina -induced extreme flooding broke a decade long of drought and made 2010 the wettest calendar year on record in the MDB. This represents a unique opportunity to develop predictive models relating flow regime to vegetation response and identify trends over long term and across a large space in a drying yet highly variable climate. Using an internally consistent method, Landsat TM and ETM+ data were used to synoptically map the extent and dynamic of surface water bodies and track the response of vegetation communities to flooding in space and time at Barmah-Millewa, the largest river red gum forest in the world and one of the icon sites in the MDB. Per pixel trajectory of surface water and vegetation index time series were used. Results show high interannual variability in number and size of flooded areas and a strong relationship with

  13. Histoplasmosis in a dog from New Brunswick

    OpenAIRE

    Tyre, Erica; Eisenbart, David; Foley, Peter; Burton, Shelley

    2007-01-01

    A young dog was presented with a history of chronic diarrhea, anorexia, and weight loss. Histoplasma capsulatum was suspected, based on cytologic examination of lymph node aspirates and peritoneal fluid, and confirmed by fungal culture. To our knowledge, this is the first case of histoplasmosis diagnosed in a dog in Atlantic Canada.

  14. Histoplasmosis: clinical syndromes and management.

    Science.gov (United States)

    Stinson, J M; Talley, P A; Thomas, F E

    1979-06-01

    The fungus Histoplasma capsulatum produces a spectrum of disease forms ranging from a benign self-limited illness to progressive disseminated disease with a 50 percent mortality rate. The drug of choice, amphotericin B, must be given intravenously over a prolonged course and carries a high incidence of toxicity. Thus, optimal managment of serious forms of histoplasmosis requires considerable clinical judgment.

  15. Dimorphic Fungal Coinfection as a Cause of Chronic Diarrhea and Pancolitis

    Directory of Open Access Journals (Sweden)

    Eduar A. Bravo

    2011-01-01

    Full Text Available Histoplasma capsulatum and Paracoccidioides brasiliensis are dimorphic fungi that cause systemic mycosis mostly in tropical South America and some areas of North America. Gastrointestinal involvement is not uncommon among these fungal diseases, but coinfection has not previously been reported. We report a patient with chronic diarrhea and pancolitis caused by paracoccidioidomycosis and histoplasmosis.

  16. Histoplasmosis presenting with solitary pulmonary nodule: Two ...

    African Journals Online (AJOL)

    2014-08-04

    Aug 4, 2014 ... characterization of a case of Histoplasma capsulatum infection in Mainland. China. Am J Trop Med Hyg 2014;90:1180‑3. 4. Wheat LJ. Histoplasmosis: A review for clinicians from non‑endemic areas. Mycoses 2006;49:274‑82. 5. Kauffman CA. Histoplasmosis: A clinical and laboratory update. Clin Microbiol.

  17. Disseminated Histoplasmosis in a 13-year-old girl: a case report

    African Journals Online (AJOL)

    EB

    caused by a fungal infection, Histoplasma capsulatum. .... Figure 3: Mesangial sclerosis, cellular proliferation and thickened glomerular capsules in keeping with ... therapy. Pediatr Infect Dis J 1996; 15: 1002-8. 5. Goodwin RA, Shapiro JL, Thurman GH,. Thurman SS, Des Prez RM: Disseminated histoplasmosis: clinical and ...

  18. A Rare Case of Primary Mucocutaneous Histoplasmosis Presenting ...

    African Journals Online (AJOL)

    ADMIN

    Histoplasma Capsulatum causes acute and/or chronic pulmonary disease but can affect other reticuloendothelial organs3. Oral lesions manifesting primarily are rare4. They tend to occur in conjunction with disseminated histoplasmosis especially in HIV-infected patients5. Recently primary mucocutaneous ulcers have been ...

  19. Disseminated cutaneous histoplasmosis in an immunocompetent adult

    Directory of Open Access Journals (Sweden)

    Manoj Harnalikar

    2012-01-01

    Full Text Available Histoplasmosis, a systemic mycosis caused by the dimorphic fungus Histoplasma capsulatum var capsulatum and Histoplasma capsulatum var duboisii is endemic to many parts of the world. The clinical manifestations range from acute or chronic pulmonary infection to a progressive disseminated disease. After initial exposure to the fungus, the infection is self-limited and restricted to the lungs in 99% of healthy individuals. The remaining 1%, however, progress to either disseminated or chronic disease involving the lungs, liver, spleen, lymph nodes, bone marrow or rarely, the skin and mucous membranes. Mucocutaneous histoplasmosis is frequently reported in patients with acquired immune deficiency syndrome (AIDS, but it is rare in immunocompetent hosts. A 60-year-old male presented with asymptomatic swelling of the hard palate and crusted papules and nodules over the extremities, face and trunk. Clinically, the diagnoses of cutaneous cryptococcosis versus histoplasmosis was considered in this patient. A chest X-ray revealed hilar lymphadenopathy. Enzyme-linked immunosorbent assay (ELISA for human immunodeficiency virus (HIV was nonreactive. Skin biopsy revealed multiple tiny intracellular round yeast forms with a halo in the mid-dermis. Culture of the skin biopsy in Sabouraud′s dextrose agar showed colonies of Histoplasma capsulatum. Despite an investigation including no evidence of underlying immunosuppression was found, he was started on IV amphotericin-B (0.5 mg/kg/day. However, the patient succumbed to his disease 2 days after presentation. We report a rare case of disseminated cutaneous histoplasmosis in an immunocompetent individual.

  20. A 30 m Resolution Surface Water Mask Including Estimation of Positional and Thematic Differences Using Landsat 8, SRTM and OpenStreetMap: A Case Study in the Murray-Darling Basin, Australia

    Directory of Open Access Journals (Sweden)

    Gennadii Donchyts

    2016-05-01

    Full Text Available Accurate maps of surface water are essential for many environmental applications. Surface water maps can be generated by combining measurements from multiple sources. Precise estimation of surface water using satellite imagery remains a challenging task due to the sensor limitations, complex land cover, topography, and atmospheric conditions. As a complementary dataset, in the case of hilly landscapes, a drainage network can be extracted from high-resolution digital elevation models. Additionally, Volunteered Geographic Information (VGI initiatives, such as OpenStreetMap, can also be used to produce high-resolution surface water masks. In this study, we derive a high-resolution water mask using Landsat 8 imagery and OpenStreetMap as well as (potential a drainage network using 30 m SRTM. Our approach to derive a surface water mask from Landsat 8 imagery comprises the use of a lower 15% percentile of Landsat 8 Top of Atmosphere (TOA reflectance from 2013 to 2015. We introduce a new non-parametric unsupervised method based on the Canny edge filter and Otsu thresholding to detect water in flat areas. For hilly areas, the method is extended with an additional supervised classification step used to refine the water mask. We applied the method across the Murray-Darling basin, Australia. Differences between our new Landsat-based water mask and the OpenStreetMap water mask regarding positional differences along the rivers and overall coverage were analyzed. Our results show that about 50% of the OpenStreetMap linear water features can be confirmed using the water mask extracted from Landsat 8 imagery and the drainage network derived from SRTM. We also show that the observed distances between river features derived from OpenStreetMap and Landsat 8 are mostly smaller than 60 m. The differences between the new water mask and SRTM-based linear features and hilly areas are slightly larger (110 m. The overall agreement between OpenStreetMap and Landsat 8 water

  1. Kiirusta, Murray-Darling / Robert Milliken

    Index Scriptorium Estoniae

    Milliken, Robert

    2009-01-01

    Veehulk Murray-Darlingu jõestikus, mis varustab veega seitsmendikku Austraaliast, on vähenemas ning veevarustuse säilimise eest peab hakkama võitlema peaminister Kevin Ruddi juhitud valitsus. Kaart

  2. Histoplasmosis meningitis in a dog: case report

    Directory of Open Access Journals (Sweden)

    Sergio Arias

    2010-12-01

    Full Text Available Canine Histoplasmosis is an American important disease mostly in the central and southwest partof the United States that has not been yet reported in Colombia. Reports in human beings suggest the presence of this parasite in Colombia. This paperintends to report a case of canine histoplasmosis which was presented to the Small Animal Hospitalof La Salle University located at Bogotá- Colombia.The dog showed clinical signs of ataxia, turning, meningoecephalitis, the CSF analysis showed yeast-likeof Histoplasma capsulatum. For our information this is one of the first reports of meningo encephalitis by Histoplasma in dogs in Colombia.

  3. A new method for quantifying and modeling large scale surface water inundation dynamics and key drivers using multiple time series of Earth observation and river flow data. A case study for Australia's Murray-Darling Basin

    Science.gov (United States)

    Heimhuber, Valentin; Tulbure, Mirela G.; Broich, Mark

    2017-04-01

    Periodically inundated surface water (SW) areas such as floodplains are hotspots of biodiversity and provide a broad range of ecosystem services but have suffered alarming declines in recent history. Large scale flooding events govern the dynamics of these areas and are a critical component of the terrestrial water cycle, but their propagation through river systems and the corresponding long term SW dynamics remain poorly quantified on continental or global scales. In this research, we used an unprecedented Landsat-based time series of SW maps (1986-2011), to develop statistical inundation models and quantify the role of driver variables across the Murray-Darling Basin (MDB) (1 million square-km), which is Australia's bread basket and subject to competing demands over limited water resources. We fitted generalized additive models (GAM) between SW extent as the dependent variable and river flow data from 68 gauges, spatial time series of rainfall (P; interpolated gauge data), evapotranspiration (ET; AWRA-L land surface model) and soil moisture (SM; active passive microwave satellite remote sensing) as predictor variables. We used a fully directed and connected river network (Australian Geofabric) in combination with ancillary data, to develop a spatial modeling framework consisting of 18,521 individual modeling units. We then fitted individual models for all modeling units, which were made up of 10x10 km grid cells split into floodplain, floodplain-lake and non-floodplain areas, depending on the type of water body and its hydrologic connectivity to a gauged river. We applied the framework to quantify flood propagation times for all major river and floodplain systems across the MDB, which were in good accordance with observed travel times. After incorporating these flow lag times into the models, average goodness of fit was high across floodplains and floodplain-lake modeling units (r-squared > 0.65), which were primarily driven by river flow, and lower for non

  4. PRIMARY CUTANEOUS HISTOPLASMOSIS PADA PASIEN DENGAN INFEKSI HUMAN IMMUNODEFICIENCY VIRUS (HIV

    Directory of Open Access Journals (Sweden)

    Nyoman Upadana

    2014-09-01

    Full Text Available Histoplasmosis adalah penyakit granulomatosa disebabkan oleh jamur dimorphic Histoplasma capsulatum. Lesi kulit merupakan bentuk sekunder, tetapi dapat juga primer pada kasus self inoculation. Kasus adalah laki-laki 27 tahun dengan benjolan sejak 3 bulan yang lalu, diawali pada kedua kaki meluas ke tangan dan wajah. Pemeriksaan kulit didapatkan papul, nodul, dan ulkus dengan tepi landai, tertutup krusta tebal. Hasil pemeriksaan CD4 adalah 4/mm3. Pemeriksaan histopatologi pada stroma tampak sebaran sel histiosit mengandung mikroorganisme, bentuk bulat, berdinding tebal, berwarna eosinofilik, dan dikelilingi oleh clear halo. Positif dengan pewarnaan periodic acid schiff dan grocott methenamic silver, dan sesuai untuk Histoplasma capsulatum. Diagnosis pasien adalah primary cutaneous histoplasmosis dan diterapi flukonazol 200 mg IV dengan respon terapi yang baik. [MEDICINA 2013;44:113-117].

  5. Non-resolving pneumonia: A rare presentation of progressive disseminated histoplasmosis

    Directory of Open Access Journals (Sweden)

    Supriya Sarkar

    2014-01-01

    Full Text Available Histoplasmosis, a fungal disease caused by Histoplasma capsulatum, is endemic in North and South America. Except few scattered cases, the disease is considered to be a non-entity in India. Furthermore, disseminated histoplasmosis is rare in the immunocompetent individuals. We report an adolescent boy presenting as middle lobe consolidation which did not respond to antibiotics. His condition deteriorated with the development of mediastinal lymphadenopathy, pleural effusion and hepatosplenomegaly. A diagnosis of progressive disseminated histoplasmosis was established by his clinical findings as well as bronchoscopic biopsy, transbronchial needle aspiration cytology and bronchoalveolar lavage culture demonstrating Histoplasma capsulatum. The case represents a unique example of progressive disseminated histoplasmosis in an immunocompetent individual in India.

  6. Disease transmission from companion parrots to dogs and cats: what is the real risk?

    Science.gov (United States)

    Bush, Jamie M; Speer, Brian; Opitz, Noel

    2011-11-01

    A number of common misconceptions exist regarding the degree of transmission from companion parrots to dogs and cats. Concern regarding bacterial, viral, fungal, and parasitic transmission is generally unfounded, because disease transmission between companion parrots and dogs and cats is not well-documented. Infections with Mycobacterium spp, Aspergillus spp, Giardia spp, Chlamydophila psittaci, Salmonella spp, Yersinia pseudotuberculosis, Cryptococcus neoformans, Histoplasma capsulatum, Cryptosporidium spp, and avian influenza are often considered possible transmissible diseases, causing pet caregivers unwarranted concerns.

  7. Disseminated histoplasmosis simulating miliary tuberculosis: a case report; Histoplasmose disseminada simulando tuberculose miliar: relato de caso

    Energy Technology Data Exchange (ETDEWEB)

    Tavares Junior, Wilson Campos; Madureira, Marcus Magalhaes; Andrade, Diego Correa de; Guimaraes, Silvana Mangeon Meireles; Queiroz, Leonardo Campos [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Hospital das Clinicas. Dept. de Radiologia e Diagnostico por Imagem]. E-mail: wilsontavaresjrmd@yahoo.fr; Avila, Renata Eliane de [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Hospital das Clinicas. Servico de Doencas Infecciosas e Parasitarias; Lambertucci, Jose Roberto [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Faculdade de Medicina. Clinica Medica

    2005-04-15

    Histoplasmosis is a disease caused by inhalation of the fungus Histoplasma capsulatum. In rare cases the disease affects immunocompetent individuals. Disseminated and severe disease is seen in immunocompromised patients. We report a case of a 45 year old immunocompromised patient presenting with weight loss and abdominal pain. Chest x-ray and computerized tomography examinations showed interstitial infiltrate and diffuse micro nodules. The initial diagnosis was miliary tuberculosis. However, the definitive diagnosis of miliary histoplasmosis was made later on. (author)

  8. Immune reconstitution syndrome in a human immunodeficiency virus infected child due to giardiasis leading to shock

    Directory of Open Access Journals (Sweden)

    Sneha Nandy

    2015-01-01

    Full Text Available Human immunodeficiency virus (HIV-associated immune reconstitution inflammatory syndrome has been reported in association with tuberculosis, herpes zoster (shingles, Cryptococcus neoformans, Kaposi′s sarcoma, Pneumocystis pneumonia, hepatitis B virus, hepatitis C virus, herpes simplex virus, Histoplasma capsulatum, human papillomavirus, and Cytomegalovirus. However, it has never been documented with giardiasis. We present a 7-year-old HIV infected girl who developed diarrhea and shock following the initiation of antiretroviral therapy, and her stool showed the presence of giardiasis.

  9. Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico.

    Science.gov (United States)

    Taylor, Maria Lucia; Ruíz-Palacios, Guillermo M; del Rocío Reyes-Montes, María; Rodríguez-Arellanes, Gabriela; Carreto-Binaghi, Laura E; Duarte-Escalante, Esperanza; Hernández-Ramírez, Aurora; Pérez, Armando; Suárez-Alvarez, Roberto O; Roldán-Aragón, Yuri A; Romero-Martínez, Rafael; Sahaza-Cardona, Jorge H; Sifuentes-Osornio, José; Soto-Ramírez, Luis E; Peña-Sandoval, Gabriela R

    2005-09-01

    Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel's ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92-99%) between them.

  10. Histoplasmosis and skin lesions in HIV: a safe and accurate diagnosis.

    Science.gov (United States)

    Moreno-Coutiño, Gabriela; Hernández-Castro, Rigoberto; Toussaint-Caire, Sonia; Montiel-Robles, Melisa; Sánchez-Pérez, Fiama Selene; Xicohtencatl-Cortés, Juan

    2015-07-01

    Human histoplasmosis is caused by the dimorphic fungus Histoplasma capsulatum. This infection can run asymptomatic or be life-threatening, depending fundamentally on the host's immune status. Immunocompromised patients can present disseminated disease to the skin, making the biopsy an accessible approach. The current diagnosis gold standard is fungal culture which takes several days or weeks to grow and must be handled in a biosafe laboratory which is avoided if we use the technique here described. We propose the use of molecular biology for diagnosis confirmation, considering it can shorten diagnosis lapse, has good specificity and sensitivity and reduces the risk of infection for the medical and laboratory personnel. Seven paraffin-embedded skin biopsy samples were included from patients with confirmed HIV and histoplasmosis diagnosis. Total DNA was isolated and molecular typing of H. capsulatum var. capsulatum. All samples were positive. This is a safe and accurate method for skin histoplasmosis diagnosis. © 2015 Blackwell Verlag GmbH.

  11. [The sources of histoplasmosis infection on the Isla de la Juventud, Cuba].

    Science.gov (United States)

    Fernández Andreu, C M; Martínez Machin, G

    1992-01-01

    The purpose of this work is to report the isolation of Histoplasma capsulatum, etiologic agent of histoplasmosis, from soil in sites inhabited by bats and chicken in the Island of Youth, Cuba. The fungus was cultured from four species of cave dwelling bats too. The identification of H. capsulatum was done by mycelial to yeast conversion and exoantigen test. It is pointed out the epidemiological value of some of these isolations in caves of great importance from the archaeological, speleological or tourist point of view, and the potential risk that they represent to human health. The authors conclude with some recommendation to prevent the infection with H. capsulatum in people who have to keep in contact with those environments.

  12. 5-Lipoxygenase deficiency impairs innate and adaptive immune responses during fungal infection.

    Directory of Open Access Journals (Sweden)

    Adriana Secatto

    Full Text Available 5-Lipoxygenase-derived products have been implicated in both the inhibition and promotion of chronic infection. Here, we sought to investigate the roles of endogenous 5-lipoxygenase products and exogenous leukotrienes during Histoplasma capsulatum infection in vivo and in vitro. 5-LO deficiency led to increased lung CFU, decreased nitric oxide production and a deficient primary immune response during active fungal infection. Moreover, H. capsulatum-infected 5-LO(-/- mice showed an intense influx of neutrophils and an impaired ability to generate and recruit effector T cells to the lung. The fungal susceptibility of 5-LO(-/- mice correlated with a lower rate of macrophage ingestion of IgG-H. capsulatum relative to WT macrophages. Conversely, exogenous LTB4 and LTC4 restored macrophage phagocytosis in 5-LO deficient mice. Our results demonstrate that leukotrienes are required to control chronic fungal infection by amplifying both the innate and adaptive immune response during histoplasmosis.

  13. Histoplasmose disseminada aguda em indivíduo imunocompetente Acute disseminated histoplasmosis in an immunocompetent patient

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    Simone Castelo Branco Fortaleza

    2004-06-01

    Full Text Available A histoplasmose é uma doença fúngica causada pela inalação de esporos de Histoplasma capsulatum. A maioria dos indivíduos normais não apresenta doença após pequena inalação, porém exposições mais prolongadas podem levar ao desenvolvimento de infecção pulmonar aguda, crônica ou disseminada. Nos pacientes imunocomprometidos a infecção é disseminada e grave. Relatamos o caso de um paciente de treze anos, imunocompetente, com febre, tosse seca e dispnéia progressiva havia dois meses. O radiograma e a tomografia computadorizada de tórax evidenciavam infiltrado intersticial com micronódulos difusos. O paciente relatava contato intenso com pássaros em sua residência. Foi submetido a biópsia pulmonar a céu aberto, que evidenciou Histoplasma capsulatum em tecido pulmonar. A cultura do fragmento da biópsia confirmou a presença de Histoplasma capsulatum sp. O paciente foi tratado com anfotericina-B por 28 dias, seguida de itraconazol por seis meses, com resolução do quadro.Histoplasmosis is a fungal disease caused by inhalation of Histoplasma capsulatum fungus. The disease does not normally affect immunocompetent individuals after a single, transient inhalation exposure. However, longer exposure may cause chronic or disseminated acute pulmonary infection. In immunocompromised patients, the infection is disseminated and severe. We report the case of a 13-year-old immunocompetent patient, presenting with fever, cough and dyspnea for one month. The chest X-ray and computed tomography scan revealed interstitial infiltrate and diffuse micronodules. The patient reported having had close and prolonged contact with birds. He was submitted to an open lung biopsy and the tissue culture was positive for Histoplasma capsulatum sp. He was treated with amphotericin B for 28 days, followed by treatment with itraconazole for 6 months, and there was complete resolution of the disease.

  14. Adrenal bilateral incidentaloma by reactivated histoplasmosis.

    Science.gov (United States)

    Lio, S; Cibin, M; Marcello, R; Viviani, M A; Ajello, L

    2000-01-01

    We report a case of bilateral adrenal incidentaloma caused by the capsulatum variety of Histoplasma capsulatum diagnosed in a 74 years old man born in and a life time resident of Treviso, Italy, with the exception of two years spent in Pakistan (1964-1966) as a well-driller. The patient was hospitalized in 1995 for alcoholic chronic hepatitis, chronic Helicobacter pylori gastritis and post-infarction ischemic cardiomyopathy. Abdominal ultrasound incidentally showed bilateral adrenal masses (the right one 6.3 cm in diameter) confirmed by computed tomography, with adrenal function within normal limits. After three months, the patient was again hospitalized due to evening fever, asthenia, anorexia, weight loss and occasional hyperhidrosis. Abdominal ultrasound showed an increase of the right adrenal lesion with normal adrenal function. Ultrasound-guided fine needle aspiration did not prove useful for diagnosis. Accordingly, a laparotomy with bilateral biopsy was performed; histology showed the presence of numerous tissue form cells of H. capsulatum variety capsulatum. Serum anti-H. capsulatum antibodies were negative. Since March, 1996, the patient was given itraconazole and his symptoms quickly regressed but the computed tomography findings, however, have not changed and the patient has adrenal hypofunction that is being treated with cortisone acetate.

  15. Involvement of intraocular structures in disseminated histoplasmosis.

    Science.gov (United States)

    Ala-Kauhaluoma, Marianne; Aho, Inka; Ristola, Matti; Karma, Anni

    2010-06-01

    To describe ocular involvement and response to treatment in a patient with human immunodeficiency virus (HIV) infection with severe progressive disseminated histoplasmosis (PDH). We report a 35-year-old HIV-infected patient seen in our clinics over a period of 4 years. During antiretroviral treatment (ART), the HIV load became undetectable at 3 months; however, CD4 T-cell count increased slowly and rose to 100 cells/microl. Histoplasma capsulatum was cultured from skin pustules, cerebrospinal fluid (CF) and aqueous humour. The patient developed central nervous system (CNS) involvement 2 months and panuveitis in both eyes 4 months after the initiation of ART. With intravenous liposomal amphotericin B followed by oral voricanozole, the chorioretinal lesions of the right eye (RE) became inactivated and magnetic resonance imaging (MRI) lesions of CNS disappeared. Relapse of the inflammation in the anterior segment of the left eye (LE) resulted in a total closure of the chamber angle and severe glaucoma. Despite medical therapy, two cyclophotocoagulations, total vitrectomy and repeated intravitreal amphotericin B injections, LE became blind. Histoplasma capsulatum was cultured from the aqueous humour after antifungal therapy of 16 months' duration. PDH with intraocular and CNS manifestations was probably manifested by an enhanced immune response against a previous subclinical disseminated infection. It seems difficult to eradicate H. capsulatum from the anterior segment of the eye in an immunocompromised patient.

  16. Disseminated histoplasmosis in two juvenile raccoons (Procyon lotor) from a nonendemic region of the United States.

    Science.gov (United States)

    Clothier, Kristin A; Villanueva, Michelle; Torain, Andrea; Reinl, Steve; Barr, Bradd

    2014-03-01

    Two 6-month-old raccoon kits, which had been rescued and fostered in preparation for return to the wild, became acutely ill and died 3 weeks before scheduled release. At necropsy, the kits had grossly enlarged livers and spleens, diffusely consolidated lungs, and generalized lymphadenopathy. Histologically, extensive infiltrates of macrophages containing yeast organisms were identified in lung, liver, kidney, spleen, lymph nodes, intestinal tissues, brain, adrenal gland, bone marrow, and thymus of both animals. Histiocytic inflammation with accompanying fibrosis was widespread, with necrotic foci evident in lungs, spleen, and intestinal sections. Fungal organisms were observed on sheep blood agar plates; however, repeated subcultures to fungal media designed to induce conidial structures for fungal identification were unsuccessful. Partial DNA sequencing of the 28S ribosomal RNA gene of the blood agar isolate identified 100% homology with Ajellomyces capsulatus (anamorphic name Histoplasma capsulatum). The kits were rescued and fostered in the San Francisco Bay area and it is likely that the exposure to H. capsulatum occurred in this area. Histoplasma sp. infection in wild mammal species is often used as an indication of spore contamination of a geographic region. Northern California is not known to be an endemic region for H. capsulatum, which is not a reportable disease in this state. The presence of severe, disseminated disease and the need for molecular identification associated with the isolate from a nonendemic region identified in the present report may indicate genetic adaptation and altered characteristics of this agent and may warrant further investigation.

  17. Antigenemia without antigenuria in a cat with histoplasmosis.

    Science.gov (United States)

    Jarchow, Anthony; Hanzlicek, Andrew

    2015-01-01

    Based on demonstration of the yeast phase of Histoplasma capsulatum on fine-needle aspirate cytology of the kidney, a 5-year-old cat was diagnosed with histoplasmosis. Urine and serum were tested for antigen via a Histoplasma antigen enzyme immunoassay. At the time of diagnosis, and on multiple occasions during antifungal treatment, antigenemia was detected without antigenuria. The cat was treated with standard therapy and achieved clinical remission. Diagnosis is most commonly made by finding the yeast phase of H capsulatum via cytology of fluid samples or cytology or histopathology of infected tissues. In certain cases this may require invasive tests. Recently, a non-invasive test, a Histoplasma antigen enzyme immunoassay, has been shown to be a sensitive test for supporting the diagnosis of histoplasmosis in cats. Urine has been considered the biologic specimen of choice for antigen testing and there is a paucity of information concerning the use of other specimens such as serum. The case herein reports a cat with antigenemia without antigenuria. These findings suggest that further research is necessary to better understand the ideal biologic sample or combination of samples as it pertains to antigen testing in cats. It also suggests that to maximize sensitivity both urine and serum may need to be tested in cats with suspected histoplasmosis.

  18. Antigenemia without antigenuria in a cat with histoplasmosis

    Directory of Open Access Journals (Sweden)

    Anthony Jarchow

    2015-11-01

    Full Text Available Case summary Based on demonstration of the yeast phase of Histoplasma capsulatum on fine-needle aspirate cytology of the kidney, a 5-year-old cat was diagnosed with histoplasmosis. Urine and serum were tested for antigen via a Histoplasma antigen enzyme immunoassay. At the time of diagnosis, and on multiple occasions during antifungal treatment, antigenemia was detected without antigenuria. The cat was treated with standard therapy and achieved clinical remission. Relevance and novel information Diagnosis is most commonly made by finding the yeast phase of H capsulatum via cytology of fluid samples or cytology or histopathology of infected tissues. In certain cases this may require invasive tests. Recently, a non-invasive test, a Histoplasma antigen enzyme immunoassay, has been shown to be a sensitive test for supporting the diagnosis of histoplasmosis in cats. Urine has been considered the biologic specimen of choice for antigen testing and there is a paucity of information concerning the use of other specimens such as serum. The case herein reports a cat with antigenemia without antigenuria. These findings suggest that further research is necessary to better understand the ideal biologic sample or combination of samples as it pertains to antigen testing in cats. It also suggests that to maximize sensitivity both urine and serum may need to be tested in cats with suspected histoplasmosis.

  19. Chronic disseminated histoplasmosis with lesions restricted to the mouth: case report Histoplasmose crônica disseminada com lesões exclusivas na boca: relato de caso

    Directory of Open Access Journals (Sweden)

    Antonio Carlos F. do Valle

    2006-04-01

    Full Text Available We report a rare case of chronic disseminated histoplasmosis with several ulcerated lesions in the oral cavity in an alcoholic patient without human immunodeficiency virus infection, with no detectable signs and symptoms of systemic disease or extraoral manifestations. Histopathological analysis revealed chronic inflammatory process with granulomas containing Histoplasma-like organisms. The isolation of Histoplasma capsulatum provided the definitive diagnosis. Treatment with itraconazole resulted in complete remission of oral lesions. As far we aware, this is the second case report of oral histoplasmosis in an HIV negative patient described in Brazil.Reportamos um caso raro de histoplasmose crônica disseminada com diversas lesões ulceradas na cavidade oral de um paciente alcoólatra, sem o vírus da imunodeficiência humana, com nenhum outro sinal detectável ou sintomas de doença sistêmica ou manifestações extra-orais. Análise histopatológica revelou processo inflamatório crônico com granulomas contendo organismos fúngicos. O isolamento do Histoplasma capsulatum forneceu o diagnóstico definitivo. Tratamento com itraconazol resultou numa remissão completa das lesões orais. De acordo com nosso conhecimento, este é o segundo caso reportado de histoplasmose oral em um paciente HIV negativo descrito no Brasil.

  20. Histoplasmosis epidémica II. Hallazgos en la Cueva del Edén (Cunday, Tolima

    Directory of Open Access Journals (Sweden)

    Elizabeth Castañeda

    1981-12-01

    Full Text Available Se informa el estudio realizado en la Cueva del Edén (Cunday, Tolima para aislar, de las tierras de la cueva y de murciélagos allí capturados, al Histoplasma capsulatum, como agente causal de una anterior epidemia de histoplasmosis ocurrida entre visitantes de la cueva. El agente se aisló en 8 de las 27 muestras de tierra y guano de murciélago. Se capturaron 233 ejemplares pertenecientes a 4 géneros de murciélagos y en los cultivos, practicados en hígado, pulmón y bazo de cien de ellos, no fue posible aislar el H. capsulatum.

  1. Histoplasmose disseminada e SIDA: importância do meio de cultivo para o espécime clínico-broncoscópico

    Directory of Open Access Journals (Sweden)

    Unis Gisela

    2004-01-01

    Full Text Available A fibrobroncoscopia é um procedimento útil no diagnóstico da histoplasmose, quando o material clínico é processado adequadamente. São apresentados dez casos de histoplasmose disseminada em pacientes com SIDA, com avaliação micológica em cultivo, nos meios Mycosel® e ágar-Sabouraud cloranfenicol de espécimes clínicos colhidos por fibrobroncoscopia. A positividade do cultivo em Mycosel® foi de 60%, enquanto no ágar-Sabouraud cloranfenicol foi de 20%, evidenciando a importância do meio seletivo no isolamento do Histoplasma capsulatum var capsulatum de espécimes clínicos potencialmente contaminados, bem como a importância das informações clínicas para o laboratório, sendo esta a chave para o diagnóstico correto.

  2. Murder your darlings: Breytenbach, die dood en die vrou | Smith ...

    African Journals Online (AJOL)

    The poet Breyten Breytenbach establishes a very specific relation between death and the art of poetry, a relation which is affirmed and elucidated in his literary essays, to such a degree that one could refer to his poetics as a death-conscious aesthetics. This is a position that is not wholly uncontroversial, especially in the ...

  3. SLAMM analysis of J.N. 'Ding' Darling NWR

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Sea-Level Affecting Marshes Model (SLAMM) report presents a model for projecting the effects of sea-level rise on coastal marshes and related habitats on J.N....

  4. Not tonight darling, I'm washing my hair

    OpenAIRE

    Goldwater, H.

    2001-01-01

    Not tonight..., was first performed at 'home', London, February 2001 [hereafter 1]. A re-worked version for The Performing House of Smallbone, London/ Kent, July 2007, curated by Sigrid Wilkinson [hereafter 2] is on the DVD documentation. Version 1 was made for a house that is also a performance venue and was made as a one-to-one performance/ 5 minutes each. Version 2 was performed for a bathroom company’s event for their clientele at two of their showrooms. This was a durational piece of...

  5. An apple-core lesion in the colon: An infectious etiology

    Directory of Open Access Journals (Sweden)

    Tanmay S Panchabhai

    2011-01-01

    Full Text Available Gastrointestinal involvement occurs in about 70% to 90% of histoplasmosis cases but is usually not the initial manifestation. We present the case of a 52-yearold HIV-positive woman who presented with gastrointestinal symptoms and an apple-core lesion on CT scan of the abdomen. The patient had been diagnosed with histoplasma colitis eight months earlier and was started on long-term itraconazole therapy. However, she prematurely discontinued treatment. A colonoscopy during the present hospitalization revealed a 3.5-cm mass, biopsies of which revealed Histoplasma capsulatum. In the present report, we discuss the differential diagnosis of apple-core lesions in the colon and the importance of keeping histoplasmosis on the differential diagnosis, especially in endemic areas like the Ohio River valley. It is equally important to ensure compliance with treatment of histoplasmosis, as well as close follow-up, as progression to colonic obstruction while on medical management has been reported.

  6. In vitro antifungal activity of miltefosine and levamisole: their impact on ergosterol biosynthesis and cell permeability of dimorphic fungi.

    Science.gov (United States)

    Brilhante, R S N; Caetano, E P; Lima, R A C; Castelo Branco, D S C M; Serpa, R; Oliveira, J S; Monteiro, A J; Rocha, M F G; Cordeiro, R A; Sidrim, J J C

    2015-10-01

    This study aimed to evaluate the in vitro activity of miltefosine and levamisole against strains of Coccidioides posadasii in the filamentous phase and strains of Histoplasma capsulatum in filamentous and yeast phases. Strains of C. posadasii in the filamentous phase (n = 22) and strains of H. capsulatum in filamentous (n = 40) and yeast phases (n = 13) were, respectively, submitted to broth macrodilution and broth microdilution methods, as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of miltefosine and levamisole. The effect of the drugs on cell membrane permeability under osmotic stress conditions and total ergosterol production were also assessed, along with quantification of extravasated molecules. The results show the inhibitory effect of levamisole and miltefosine against C. posadasii and H. capsulatum and the effect of these drugs on ergosterol synthesis and the permeability of the plasma membrane using subinhibitory concentrations against strains subjected to osmotic stress. Levamisole was also able to cause the release of nucleic acids. Miltefosine and levamisole are capable of inhibiting the in vitro growth of C. posadasii and H. capsulatum, probably by altering the permeability of the cellular membrane. This work presents alternatives for the treatment of histoplasmosis and coccidioidomycosis, raising the possibility of the use of miltefosine and levamisole as adjuvants in antifungal therapy, providing perspectives for the design of in vivo studies. © 2015 The Society for Applied Microbiology.

  7. Brotes de histoplasmosis registrados en el área andina colombiana

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    Nelly Ordóñez

    1997-06-01

    Full Text Available A review of 12 histoplasmosis outbreaks that occurred in the Colombian Andean region, are presented. According to their location, 3 were detected in each of the following states: Tolima, Caldas and Antioquia and 1 each in Cundinamarca, Boyacá and Risaralda. In 9 of the outbreaks it was possible to trace the following activities which led to the infection: visits to bat-inhabited caves, removing debris incide a hollowed tree, demolition of an old house, use of contaminated lime for fertilization, handling chickenmanure fettilised soil and the severe pruning of coffee trees. Histoplasma capsulatum var. capsulatum was isolated from environmental samples in 5 of the 8 outbreaks where it was feasible to obtain material for culture and animal inoculation. It was found that 332 persons had been in contact with the various infectious sources, including the 11 index cases (2 outbreaks had the same index case. All of them were studied by means of immunological tests designed to detect antibodies; based on the findings of reactive tests, al1 the index cases and 140 (42% of those exposed had antibodies indicative of infection by the fungus. The above cases reveal the importance of histoplasmosis in immunocompetent individuals who, for occupational or recreational reasons, are exposed to infectious aerosols generated when disturbing the natural habitat of H. capsulatum var. capsulatum.

  8. Histoplasmosis epidémica II. Hallazgos en la Cueva del Edén (Cunday, Tolima)

    OpenAIRE

    Elizabeth Castañeda; Alberto Cadena; Alvaro Aguilera; Luz Helena Sanín; Liberto Ajello; Robert Weeks; Vladimir Corredor; Mauricio Restrepo

    1981-01-01

    Se informa el estudio realizado en la Cueva del Edén (Cunday, Tolima) para aislar, de las tierras de la cueva y de murciélagos allí capturados, al Histoplasma capsulatum, como agente causal de una anterior epidemia de histoplasmosis ocurrida entre visitantes de la cueva. El agente se aisló en 8 de las 27 muestras de tierra y guano de murciélago. Se capturaron 233 ejemplares pertenecientes a 4 géneros de murciélagos y en los cultivos, practicados en hígado, pulmón y bazo de cien de ellos, no f...

  9. Update: outbreak of acute febrile respiratory illness among college students--Acapulco, Mexico, March 2001.

    Science.gov (United States)

    2001-05-11

    On March 30, CDC was notified by the Pennsylvania Department of Health of an acute febrile respiratory illness characterized by fever, chills, dry cough, chest pain, and headache among college students who traveled to Acapulco during March 2001. Initial laboratory testing indicated that most students had histoplasmosis, an infection caused by the soil-inhabiting fungus, Histoplasma capsulatum. While in Acapulco, most ill students had stayed at the Calinda Beach Hotel. This report updates the investigation of the outbreak and presents possible evidence of ongoing transmissions.

  10. Seroprevalencia de micosis pulmonares en pacientes con sintomatología de enfermedad respiratoria crónica baja: Alto Huallaga, Perú 2004

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    Walter Gómez

    2006-06-01

    Full Text Available Objetivo: Detectar por serología la presencia de micosis de localización pulmonar (Paracoccidioides brasiliensis, Aspergillus sp. e Histoplasma capsulatum, en pacientes con síntomas de enfermedad respiratoria crónica. Diseño: Estudio transversal. Lugar: Alto Huallaga (selva de los departamentos de Huánuco y San Martín, Perú. Pacientes: Se incluyó personas procedentes de seis distritos (Rupa Rupa, Tocache, Uchiza, Santa Lucia, Aucayacu y Puerto Inca - Sungaro con diagnóstico de enfermedad respiratoria crónica baja, tres episodios o más de bronquitis al año y/o tos con expectoración por más de tres meses y en quienes se descartó tuberculosis pulmonar mediante baciloscopia directa. Intervenciones: Por cada persona se llenó una ficha clínica-epidemiológica durante abril a septiembre de 2004, seguido de la toma de una muestra de suero (10 mL para las pruebas de inmunodifusión para Histoplasma capsulatum, Paraccocidiodes brasiliensis, Aspergilus sp, y Aspergillus fumigatus. Principales medidas de resultados: Seroprevalencia de micosis a Histoplasma capsulatum, Aspergilosis spp. y Paracoccidioides brasiliensis. Resultados: De 640 casos, 327 (51,1% fueron de sexo masculino, edad promedio 37,1 años y tiempo de enfermedad de 7,4 meses. La seroprevalencia de micosis fue 1,7% (11/640: 4 Histoplasma capsulatum y 7 Aspergilosis spp. No se obtuvo casos positivos de Paracoccidioides brasiliensis y, en los casos positivos, solo se encontró asociación con 'baja de peso' (p = 0,004. Conclusión: Si bien la frecuencia micoserológica obtenida fue baja, se sugiere en esta zona endémica considerar a las micosis dentro del diagnóstico diferencial de casos con enfermedades respiratorias crónicas bajas, así como la utilización de técnicas de inmunodifusión para el diagnóstico de estas etiologías.

  11. Seroprevalencia de micosis pulmonares en pacientes con sintomatología de enfermedad respiratoria crónica baja: Alto Huallaga, Perú 2004

    OpenAIRE

    Walter Gómez; Miriam Guevara; Carlos Cortegana; Pedro Obregon; Juan Motta; Nancy Antholveg

    2006-01-01

    Objetivo: Detectar por serología la presencia de micosis de localización pulmonar (Paracoccidioides brasiliensis, Aspergillus sp. e Histoplasma capsulatum), en pacientes con síntomas de enfermedad respiratoria crónica. Diseño: Estudio transversal. Lugar: Alto Huallaga (selva de los departamentos de Huánuco y San Martín), Perú. Pacientes: Se incluyó personas procedentes de seis distritos (Rupa Rupa, Tocache, Uchiza, Santa Lucia, Aucayacu y Puerto Inca - Sungaro) con diagnóstico de enfermedad r...

  12. Seroprevalencia de micosis pulmonares en pacientes con sintomatología de enfermedad respiratoria crónica baja. Alto Huallaga, Perú 2004

    OpenAIRE

    Gómez, Walter; Hospital Tingo María – EsSalud. Huanuco, Perú; Guevara, Miriam; Laboratorio de Micología, Instituto Nacional de Salud. Lima, Perú; Cortegana, Carlos; Hospital Tingo María – EsSalud. Huanuco, Perú; Obregon, Pedro; Hospital Huanuco - Essalud. Huanuco, Perú; Motta, Juan; Hospital Tingo María – EsSalud. Huanuco, Perú; Antholveg, Nancy; Hospital Tingo María – EsSalud. Huanuco, Perú

    2013-01-01

    Objetivo: Detectar por serología la presencia de micosis de localización pulmonar (Paracoccidioides brasiliensis, Aspergillus sp. e Histoplasma capsulatum), en pacientes con síntomas de enfermedad respiratoria crónica. Diseño: Estudio transversal. Lugar: Alto Huallaga (selva de los departamentos de Huánuco y San Martín), Perú. Pacientes: Se incluyó personas procedentes de seis distritos (Rupa Rupa, Tocache, Uchiza, Santa Lucia, Aucayacu y Puerto Inca - Sungaro) con diagnóstico de enfermedad r...

  13. CondiÃÃes clÃnicas orais de pacientes com histoplasmose disseminada e AIDS em hospital de referÃncia de Fortaleza

    OpenAIRE

    Aglaà Francelino Freitas

    2010-01-01

    A histoplasmose disseminada (HD) Ã uma grave infecÃÃo oportunista, causada por Histoplasma capsulatum, frequentemente observada em pacientes com aids em avanÃada imunossupressÃo atendidos em serviÃo de assistÃncia a pessoas vivendo com HIV/aids em Fortaleza, CearÃ. Como outras infecÃÃes oportunistas, a histoplasmose pode ser precocemente identificada pela presenÃa de manifestaÃÃes especÃficas na cavidade oral. Para conhecer a frequÃncia e caracterÃsticas das manifestaÃÃes orais de HD associad...

  14. Reactividad a la histoplasmina en trabajadores de granjas avícolas en la provincia de Ciego de Avila, Cuba Hipersensitivity to histoplasmin in poultry farmers in the province of Ciego de Avila, Cuba

    Directory of Open Access Journals (Sweden)

    Miguel Suarez Hernandez

    1992-08-01

    Full Text Available Se realizo una encuesta epidemiológica mediante la prueba intradérmica de histoplasmina a 392 trabajadores de granjas avícolas y 265 de diferentes centros ocupacionales considerados sin riesgo profesional de exposición a Histoplasma capsulatum, agente etiológico de la histoplasinosis. Entre los trabajadores del primer grupo se detectó un 28,8% de positividad, mientras que en el segundo grupo se encontro un 13,2% de casos positivos; desde el punto de vista estadístico existió diferencia significativa entre ambos, lo que demuestra que los trabajadores de granjas avícolas son un grupo de riesgo de adquirir la infección por H capsulatum. Entre los trabajadores del grupo I se encontro un porcentaje de reactividad más alto en aquellos grupos ocupacionales que estaban más en contacto con las excretas de las aves. El tiempo de trabajo en la granja también parece influir en la reactividad a la prueba.An epidemiological survey with histoplasmin skin test was performed in 392 poultry farmers and 265 workers considered without occupational risk of exposition to Histoplasma capsulatum, etiologic agent of histoplasmosis. The results were positive in 28.8% and 13.2% in both groups respectively. Statistically, there was a significant difference between the two groups, so it can be considered that poultry farmers are in occupational risk of infection with H. capsulatum. In the first group, the workers which are more closely related with chicken manure showed a higher reactivity to histoplasmin skin test. The working time in the farms seems to influence in the test reactivity too.

  15. Fuentes de infeccion de histoplasmosis en la Isla de la Juventud, Cuba Sources of infection of histoplasmosis in the Island of Youth, Cuba

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    Carlos Manuel Fernandez Andreu

    1992-10-01

    Full Text Available El propósito del presente trabajo es reportar el aislamiento de Histoplasma capsulation, agente etiológico de la histoplasmosis, a partir del suelo de lugares habitados por murciélagos y gallinas en la Isla de la Juventud, Cuba. El hongo fue cultivado también a partir de los órganos de cuatro especies de murciélagos capturados en cuevas. La identificación de H. capsulatum se realizó mediante la conversión de la fase miceliana a levadura y por el test de exoantígenos. Se señala el valor epidemiológico de estos hallazgos en cuevas de gran importancia arqueológica, espeleológica y turística, así como el riesgo potencial que representan para la salud humana. Los autores concluyen con recomendaciones para evitar la infección por H. capsulatum en aquellas personas que por determinadas razones tengan que estar en contacto con estos ambientes contaminados.The purpose of this work is to report the isolation of Histoplasma capsulatum, etiologic agent of histoplasmosis, from soil in sites inhabited by bats and chicken in the Island of Youth, Cuba. The fungus was cultured from four species of cavedwelling bats too. The identification of H. capsulatum was done by mycelial to yeast conversion and exoantigen test. It is pointed out the epidemiological value of some of these isolations in caves of great importance from the archeologic, speleologic or touristic point of view; and the potential risk that they represent to human health. The authors conclude with some recommendation to prevent the infection with H. capsultatum in people who have to keep in contact with those environments.

  16. [Histoplasmosis - an unusual African souvenir].

    Science.gov (United States)

    Raselli, C; Reinhart, W H; Fleisch, F

    2013-02-01

    A 24-year-old woman (student of biology) was part of a study group in Uganda. She developed fever and headache, which was empirically treated as malaria. After she had returned to Switzerland, a chest x-ray showed bilateral miliary nodular infiltrates. In assumption of an atypical pneumonia, she was treated with levofloxacin, although without success. On admission, she was in a bad general condition and was markedly dyspneic. Rales were heard over both lungs. CRP, liver enzymes and LDH were elevated. A lung function test revealed a marked impairment of the diffusion capacity. The chest x-ray showed a progression of the lung infiltrates. The informal medical data exchange among the group members by a virtual social network abbreviated our diagnostic workup substantially, since we heard that histoplasmosis had been assumed in another group member. It turned out that the affected persons had visited a colony of bats living in a cave inside the trunk of a tree. Antibodies against Histoplasma capsulatum were positive. We began a treatment with itraconazole. The condition improved gradually; chest x-ray and lung function normalized after the 8 week treatment. Histoplasmosis with such a severe course is rare in immunocompetent humans, which indicates that the inoculum must have been very high. Soil contaminated with bats guano favours the proliferation of Histoplasma capsulatum. Our case is also an illustration of how the widespread use of electronic media can sometimes facilitate our work. © Georg Thieme Verlag KG Stuttgart · New York.

  17. Associação da rifampicina à anfotericina B no tratamento da paracoccidioidomicose: resultados em três pacientes tratados Association of Amphotericin B and Rifampicin in the treatment of paracoccidioidomycosis. Report of efficacy in three cases

    Directory of Open Access Journals (Sweden)

    Bodo Wanke

    1984-08-01

    Full Text Available Trabalhos experimentais demonstraram que a anfotericina B, desorganizando funcionalmente a membrana celular fúngica, permite a penetração da rifampicina no citoplasma e sua conseqüente ação contra Histoplasma capsulatum, Blastomyces dermatitidis e Candida albicans. Com metade das doses habituais' de anfotericina B associada à rifampicina conseguem-se melhores resultados do que com a anfotericina B isoladamente em doses plenas. Os Autores discutem as possíveis aplicações desta associação no tratamento da paracoccidioidomi-cose e apresentam 3 casos desta micose em que a inatividade clínica e micológica só foi obtida após o emprego combinado destas drogas.Experimental data have shown that low concentration of amphotericin B disrupts of the fungal cellular membranes, enhancing entrance of rifampicin into citoplasm, where it acts specifically as an antifungal agent against Histoplasma capsulatum, Blastomyces dermatitidis and Candida albicans. Amphotericin B in half dosage combined with rifampicin is more effective than when used alone in full dosage. The Authors discuss the possible indications of this association in the therapy of paracoccidioidomycosis, and report its efficacy in three cases of this disease only controlled after its use.

  18. Histoplasmose pulmonar cavitária crônica Chronic cavitary pulmonary histoplasmosis

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    José Wellington Alves dos Santos

    2009-11-01

    Full Text Available A histoplasmose é uma micose sistêmica causada pelo fungo dimórfico térmico Histoplasma capsulatum, que pode ser isolado a partir de solo contaminado com excrementos de aves e morcegos. Dentre as apresentações clínicas dessa doença, a histoplasmose pulmonar cavitária crônica (HPCC é uma manifestação rara. O diagnóstico diferencial com tuberculose deve ser realizado em pacientes que apresentam lesões cavitadas nos segmentos pulmonares superiores. É relatado um caso de uma paciente com HPCC que apresentou dispneia progressiva e piora do padrão radiológico em quatro anos de evolução da doença.Histoplasmosis is a systemic mycosis caused by the thermally dimorphic fungus Histoplasma capsulatum, which can be isolated from soil contaminated with droppings from birds or bats. Chronic cavitary pulmonary histoplasmosis is one of the rarest clinical presentations of this disease. The differential diagnosis with tuberculosis should be made in patients presenting with cavitated lesions in upper lung segments. We report the case of a female patient with chronic cavitary pulmonary histoplasmosis who had presented with progressive dyspnea and worsening of the radiological pattern over a four-year period.

  19. Acute Disseminated Histoplasmosis with Atypical Lymphocytosis in an Immunocompetent Host

    Directory of Open Access Journals (Sweden)

    Ayman Elbadawi, MD

    2017-01-01

    Full Text Available 65 year-old-male presented with a one-week history of high grade fever, fatigue and confusion which began abruptly two days after a cystoscopy procedure. Past medical history included pulmonary sarcoidosis diagnosed by mediastinal lymph biopsy, diabetes and hypertension. On admission he was febrile and confused with stable vital signs. Initial workup included negative Head CT and lumbar puncture. Blood work revealed normal metabolic and liver function tests with progressive anemia, thrombocytopenia and atypical lymphocytosis of 15–20%. Blood, urine and respiratory cultures all were negative for bacteria and. A bone marrow biopsy was done given the abnormal lymphocytes in peripheral smear, revealing budding yeast consistent with Histoplasma capsulatum. Histoplasma antigen was positive in urine and eventually blood and bone marrow grew H. capsulatum. Patient was started on amphotericin-B for diagnosis of disseminated histoplasmosis. After a 2 week period of amphotericin B, patient was switched to oral Itraconazole to complete 12 months course of treatment.

  20. Mycological study for a management plan of a neotropical show cave (Brazil

    Directory of Open Access Journals (Sweden)

    Erika Linzi Silva Taylor

    2013-09-01

    Full Text Available Caves are stable environments with characteristics favoring the development of microorganisms. The allocthonous input of organic matter and microbes into the warm Neotropical caves may favor the development of filamentous fungi, including pathogenic species. Histoplasma capsulatum is a pathogenic species commonly found in caves and associated with bat and bird guano. Many Brazilian caves have been historically visited due to scenic and religious tourism. The objective of this study was to perform a microbiology study for a management plan of a show cave in Brazil, focusing on the presence and distribution of pathogenic and opportunistic fungi in the cave. Statistic analysis was used to verify the influence of touristic activity on airborne fungi spore load. Fungi were isolated from air and guano in Lapa Nova Cave. Samples were obtained through serial dilution, direct and settle plate techniques. For H. capsulatum, samples were incubated in specific media and conditions. Airborne fungal spore load was compared prior and during visitation and statistically analyzed. A total of 2,575 isolates from the genera Aspergillus, Calcarisporium, Chaetomium, Cladosporium, Curvularia, Emericella, Eurotium, Fusarium, Geotrichum, Gliocladium, Mucor, Purpureocillium, Paecilomyces, Penicillium, Rhizopus and Trichoderma were identified. Histoplasma capsulatum was not isolated from the cave. Eleven opportunistic species were identified. Significant (p<0.05 variations on fungal richness in the air occurred due to cave visitation. Areas of potential microbiologic risks were indicated and management actions suggested. The results suggest a diverse community inhabiting the cave. Possible opportunistic species should be monitored in show caves and microbiota should always be included in the elaboration of cave management plans. This is the first detailed microbiologic study for a management plan of a show cave in the country. It provides relevant information for future

  1. Multiple opportunistic fungal infections in an individual with severe HIV disease: A case report.

    Science.gov (United States)

    Almeida-Silva, Fernando; Damasceno, Lisandra Serra; Serna, Maria Jose Buitrago; Valero, Clara; Quintella, Leonardo Pereira; Almeida-Paes, Rodrigo; Muniz, Mauro de Medeiros; Zancope-Oliveira, Rosely Maria

    2016-01-01

    Fungal infections have been commonly diagnosed in individuals with advanced HIV disease. Cryptococcosis, pneumocystosis, and histoplasmosis are the most frequent systemic mycoses in people suffering from HIV/AIDS. We report a case of multiple fungal infections in an advanced AIDS-patient. A 33-year-old HIV-positive man from Brazil was hospitalized due to diarrhea, dyspnea, emaciation, hypoxemia, extensive oral thrush, and a CD4+ T lymphocyte count of 20cells/mm(3). Honeycombed-structures consistent with Pneumocystis jirovecii were observed by direct immunofluorescence in induced sputum. Cryptococcus neoformans was recovered from respiratory secretion and cerebrospinal fluid cultures. Histopathology of the bone marrow also revealed the presence of Histoplasma capsulatum. Molecular assays were performed in a sputum sample. Nested-PCR confirmed the presence of P. jirovecii and H. capsulatum; qPCR multiplex was positive for C. neoformans and H. capsulatum. With the treatment of antifungal drugs the patient progressed satisfactorily. The diagnosis of several systemic mycoses demonstrates the vulnerability of advanced AIDS-patients. Thus, the detection of AIDS cases in the early stages of infection is necessary for a prompt and adequate introduction of HAART therapy, and the use of prophylaxis to control opportunistic infections. Copyright © 2015 Asociación Española de Micología. Published by Elsevier Espana. All rights reserved.

  2. Typical Facial Lesions: A Window of Suspicion for Progressive Disseminated Histoplasmosis—A Case of Asian Prototype

    Directory of Open Access Journals (Sweden)

    Prasan K. Panda

    2016-01-01

    Full Text Available Histoplasmosis is caused by a dimorphic fungus Histoplasma capsulatum in endemic areas, mainly America, Africa, and Asia. In India, it is being reported from most states; however, it is endemic along the Ganges belt. We report a case of an apparently immunocompetent male who presented with 3-month history of fever, cough, and weight loss with recent onset odynophagia and had hepatosplenomegaly and mucocutaneous lesions over the face. The differential diagnosis of leishmaniasis, tuberculosis, leprosy, fungal infection, lymphoproliferative malignancy, and other granulomatous disorders was considered, but he succumbed to his illness. Antemortem skin biopsy and bone marrow aspiration along with postmortem liver, lung, and spleen biopsy showed disseminated histoplasmosis. This case highlights the need for an early suspicion of progressive disseminated histoplasmosis in the presence of classical mucocutaneous lesions even in an immunocompetent patient suffering from a febrile illness. Cure rate approaches almost 100% with early treatment, whereas it is universally fatal if left untreated.

  3. Histoplasmosis en caninos y felinos: signos clínicos, métodos de diagnóstico y tratamiento

    Directory of Open Access Journals (Sweden)

    G. E. Martínez Cepeda

    2017-06-01

    Full Text Available La histoplasmosis es una enfermedad zoonótica, causada por el hongo dimórfico Histoplasma capsulatum, endémica en zonas tropicales, subtropicales y templadas de América y África. Suelos con abundante materia orgánica, excremento de aves y guano de murciélagos constituyen el nicho ecológico de este microorganismo. Esta micosis puede afectar a gran variedad de mamíferos. En esta revisión bibliográfica se aborda el tema de la histoplasmosis canina y felina, haciendo referencia, principalmente, a los métodos diagnósticos directos e indirectos empleados para su identificación. Además, se describen las manifestaciones clínicas características de cada fase de la enfermedad y su tratamiento y se presenta un algoritmo de diagnóstico.

  4. [Colorectal diseases in AIDS patients and endoscopic findings].

    Science.gov (United States)

    Averbach, M; Cutait, R; Corrêa, P; Duarte, M I; Leite, K; Borges, J L

    1998-01-01

    In order to establish which are the most frequent endoscopic detectable colorectal disorders in AIDS patients with intestinal complaints we analysed 236 colonoscopies in a series of 186 patients. The colonoscopic procedure was always followed by biopsies, even in the absence of macroscopic lesions. The most frequent diagnosis was colitis due to citomegalovirus infection observed in 64 exams (27.1%) and presented with an inflammatory pattern with ulcers, followed by Cryptosporidium sp., found in 31 exams (13.1%). This infection, conversely, presented as an inflammatory non-ulcerative lesion. Others pathogens found in lower frequency were: Mycobacterium sp., Histoplasma capsulatum, Herpes simplex, Isospora sp., Giardia sp., Candida sp. and Campilobacter sp.. Neoplastic lesions, mostly Kaposy's sarcoma, were detected in 10 of the colonoscopies (4.2%). The most frequent colorectal disorders in AIDS patients detected by endoscopic procedures are citomegalovirus and Cryptosporidium infection. Biopsies are always necessary to confirm the endoscopic diagnosis and to identify the presence of associated pathogens.

  5. Lymphocyte transformation in presumed ocular histoplasmosis

    Energy Technology Data Exchange (ETDEWEB)

    Ganley, J.P.; Nemo, G.J.; Comstock, G.W.; Brody, J.A.

    1981-08-01

    Lymphocytes from individuals with inactive macular disciform lesions of presumed ocular histoplasmosis challenged with three histoplasmin antigens incorporated tritiated thymidine at a significantly higher rate than histoplasmin-stimulated lymphocytes of matched control and peripheral scar groups. This finding is consistent with the etiologic association of the disciform ocular syndrome and previous systemic infection with Histoplasma capsulatum. The disciform group had a higher mean response than the other two groups to pokeweed mitogen but not to phytohemagglutinin and had higher mean counts per minute to the specific antigens Toxoplasma gondii, Blastomyces dermatitidis, Cryptococcus neoformans, Mycobacterium tuberculosis, M battery, and M gaus, but not to Candida albicans. These data would suggest that individuals with the disciform lesion of presumed ocular histoplasmosis have a hyperreactive cellular immune response; this response may play an important role in the development of the disciform.

  6. Orofacial manifestations of histoplasmosis in HIV-positive patients: a case report.

    Science.gov (United States)

    Gomes Ferreira, O; Vieira Fernandes, A; Sebastião Borges, A; Simão Ferreira, M; Mota Loyola, A

    2001-01-01

    Amongst the main opportunistic diseases that affect the HIV-positive patient, histoplasmosis is found. This systemic mycosis caused by the fungus Histoplasma capsulatum has the capacity to disseminate from the lung to the skin and oral mucosa. Oral lesions of histoplasmosis can be found with ulcerated or nodular aspect, being always very painful and infiltrating the mucosa. When they are present in the mouth, they strongly indicate the presence of some kind of immunosuppression. This study shows the disease's evolution in an HIV-positive patient, who presented several ulcerated lesions in the oral cavity and facial skin. The symptomatology and clinical aspects of the lesions were not specific for the disease, and due to this, the diagnosis was obtained by cytological smear and oral biopsy. The results of the exams defined the disseminated picture of the infection. The treatment plan involved the use of amphotericin B for the lesions' remission, and, following this, itraconazole was administered in the maintenance phase.

  7. Impairment of Neutrophil Migration to Remote Inflammatory Site during Lung Histoplasmosis

    Directory of Open Access Journals (Sweden)

    Alexandra I. Medeiros

    2015-01-01

    Full Text Available Histoplasma capsulatum (Hc induces a pulmonary disease in which leukotrienes promote activation and recruitment of effectors cells. It is also well-recognized that leukotriene B4 (LTB4 and platelet-activating factor (PAF induce leukocyte recruitment to inflammatory sites. We investigated the impact of pulmonary Hc infection on PMN migration to a remote inflammatory site. Our results show that pulmonary Hc infection impairs LTB4- or PAF-stimulated PMN recruitment to air pouch. Yet, remote inflammation did not modify PMN numbers in the bronchoalveolar lavage fluid (BALF of Hc-infected mice. Interestingly, the concomitant administration of PAF and LTB4 receptor antagonists inhibited PMN recruitment to both BALF and the remote site, demonstrating cooperation between both mediators. Along that line, our results show that PAF-elicited PMN chemotaxis was abrogated in 5-lipoxygenase-deficient animals. These results suggest caution in the indiscriminate use of anti-inflammatory drugs during infectious diseases.

  8. Virulence Factors IN Fungi OF Systemic Mycoses

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    KUROKAWA Cilmery Suemi

    1998-01-01

    Full Text Available Pathogenic fungi that cause systemic mycoses retain several factors which allow their growth in adverse conditions provided by the host, leading to the establishment of the parasitic relationship and contributing to disease development. These factors are known as virulence factors which favor the infection process and the pathogenesis of the mycoses. The present study evaluates the virulence factors of pathogenic fungi such as Blastomyces dermatitidis, Coccidioides immitis, Cryptococcus neoformans, Histoplasma capsulatum and Paracoccidioides brasiliensis in terms of thermotolerance, dimorphism, capsule or cell wall components as well as enzyme production. Virulence factors favor fungal adhesion, colonization, dissemination and the ability to survive in hostile environments and elude the immune response mechanisms of the host. Both the virulence factors presented by different fungi and the defense mechanisms provided by the host require action and interaction of complex processes whose knowledge allows a better understanding of the pathogenesis of systemic mycoses.

  9. Thermally Dimorphic Human Fungal Pathogens--Polyphyletic Pathogens with a Convergent Pathogenicity Trait.

    Science.gov (United States)

    Sil, Anita; Andrianopoulos, Alex

    2014-11-10

    Fungi are adept at changing their cell shape and developmental program in response to signals in their surroundings. Here we focus on a group of evolutionarily related fungal pathogens of humans known as the thermally dimorphic fungi. These organisms grow in a hyphal form in the environment but shift their morphology drastically within a mammalian host. Temperature is one of the main host signals that initiates their conversion to the "host" form and is sufficient in the laboratory to trigger establishment of this host-adapted developmental program. Here we discuss the major human pathogens in this group, which are Blastomyces dermatiditis, Coccidioides immitis/posadasii, Histoplasma capsulatum, Paracoccidioides brasiliensis/lutzii, Sporothrix schenckii, and Talaromyces marneffei (formerly known as Penicillium marneffei). The majority of these organisms are primary pathogens, with the ability to cause disease in healthy humans who encounter them in endemic areas. Copyright © 2015 Cold Spring Harbor Laboratory Press; all rights reserved.

  10. Histoplasmosis Presenting as Granulomatous Hepatitis: Case Report and Review of the Literature

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    Nancy A. Rihana

    2014-01-01

    Full Text Available Background. Histoplasma capsulatum is the most common endemic mycosis in the United States and is a frequent cause of opportunistic infection in immunodeficient hosts. Histoplasmosis is most often self-limiting and goes unrecognized in the immunocompetent population but can progress to disseminated histoplasmosis in patients with an impaired immune system. Liver involvement as a part of disseminated histoplasmosis which usually originates in the lung is well known. However, extrapulmonary hepatic histoplasmosis as a primary manifestation is extremely rare. Case Presentation. We report a rare case of histoplasmosis that presented as persistent fever and abnormal liver function tests in a 66-year-old female with rheumatoid arthritis, receiving infliximab. Conclusion. Emphasizing histoplasmosis as a major cause of acute granulomatous hepatitis and fever of unknown origin in cell mediated immunodeficient population, this case highlights the need for high index of suspicion and the importance of prompt diagnosis since any delay of treatment can be life threatening in this population.

  11. Histoplasmosis diseminada, linfopenia, y síndrome de Sjögren Disseminated histoplasmosis, lymphopenia and Sjögren´s syndrome

    OpenAIRE

    Horacio F. Rodrigo; Rodolfo N. Stavile; Sabino Deleo

    2012-01-01

    El síndrome de Sjögren es una enfermedad autoinmune caracterizada por disminución de las secreciones de las glándulas exocrinas; puede presentar también diversas alteraciones hemáticas, entre ellas linfopenia. Presentamos el caso de un varón de 28 años que consultó por cefalea de un mes de evolución a la que se agregaron en las últimas 48 horas vómitos y fiebre. Presentaba lesiones en piel de tronco y cara; no tenía rigidez de nuca. Se demostró infección por Histoplasma capsulatum var. capsul...

  12. Histoplasmosis diseminada, linfopenia, y síndrome de Sjögren

    OpenAIRE

    Horacio F. Rodrigo; Rodolfo N. Stavile; Sabino Deleo

    2012-01-01

    El síndrome de Sjögren es una enfermedad autoinmune caracterizada por disminución de las secreciones de las glándulas exocrinas; puede presentar también diversas alteraciones hemáticas, entre ellas linfopenia. Presentamos el caso de un varón de 28 años que consultó por cefalea de un mes de evolución a la que se agregaron en las últimas 48 horas vómitos y fiebre. Presentaba lesiones en piel de tronco y cara; no tenía rigidez de nuca. Se demostró infección por Histoplasma capsulatum var. capsul...

  13. [Outbreak of histoplasmosis in province of Neuquén, Patagonia Argentina].

    Science.gov (United States)

    Calanni, Liliana María; Pérez, Rufina Ana; Brasili, Susana; Schmidt, Norma Graciela; Iovannitti, Cristina Adela; Zuiani, María Fernanda; Negroni, Ricardo; Finquelievich, Jorge; Canteros, Cristina Elena

    2013-01-01

    In Argentina, there are no reports of autochthonous cases of histoplasmosis in the southern regions of the country. To report a histoplasmosis outbreak in Zapala town, Province of Neuquén, Patagonia Argentina. We evaluated the clinical and epidemiological characteristics of 5 patients involved in the outbreak. Environmental studies were conducted to determine the source of infection. The genetic profile of Histoplasma capsulatum strains isolated from the index case (IC) were compared with clinical isolates from Argentinean patients not related to the outbreak, using RAPD-PCR with primers 1281-1283. The patients were residents of Zapala, and had not visited other geographical areas before. All patients had an influenza-like syndrome, and X-ray revealed disseminated micronodular images throughout the lung parenchyma. The IC needed specific antifungal therapy; the remaining 4 patients had mild symptoms, and did not require therapy. All of them had a good clinical outcome. Strains of H. capsulatum isolated from blood culture and lung biopsy of the IC showed a genetic profile different from other strains analyzed. The presence of the fungus in the environment was demonstrated by the detection of anti-Histoplasma antibodies in BALB/c mice inoculated with soil obtained in a culvert where workers had dug up earth after a landslide. This outbreak suggests the histoplasmosis endemic area is under the 38° S parallel. Patients from Neuquén, Patagonia Argentina, with compatible symptoms of histoplasmosis should be tested, regardless of their travel or exposure history. Copyright © 2012 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.

  14. Rupioid histoplasmosis: first case reported in an AIDS patient in Argentina Histoplasmose rupioide: primeiro caso relatado em um paciente com AIDS na Argentina

    Directory of Open Access Journals (Sweden)

    Marcelo Corti

    2010-10-01

    Full Text Available Disseminated histoplasmosis is a relatively common AIDS-defining illness, occurring in almost 4% of patients living in endemic areas and it may be the first clinical expression of the HIV infection. A broad spectrum of clinical skin lesions associated with Histoplasma capsulatum infection have been described in AIDS patients, such as erythematous macules, papules, nodules, and pustules. Herpetic, acneiform, erythema multiforme-like, molluscum contagiosum-like, vasculitic, and exfoliative forms have also been reported. To our knowledge, this is the first case of disseminated histoplasmosis in an AIDS patient presented as a rupioid eruption.A histoplasmose disseminada é uma das doenças associadas à AIDS e relativamente comum, ocorrendo em quase 4% dos pacientes que vivem em áreas endêmicas e pode ser a primeira expressão clínica da infecção pelo HIV. Amplo espectro de lesões de pele associadas com a infecção pelo Histoplasma capsulatum têm sido descritas nos pacientes com AIDS, tais como máculas eritematosas, pápulas, nódulos e pústulas. Foram também relatadas lesões herpéticas, acneiformes, similares ao eritema multiforme, similares ao molusco contagioso, vasculíticas e esfoliativas. Em nosso conhecimento este é o primeiro caso de histoplamose disseminada em paciente com AIDS que se apresenta como erupção de aspecto rupióide.

  15. Histoplasmin reaction. Comparison of a polysaccharide antigen to the filtrate antigen

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    Sérgio Di Camilo FAVA

    1997-09-01

    Full Text Available This work was planned by taking into account all the knowledge accumulated from the immunological study of paracoccidioidomycosis. It aimed at comparing a polysaccharide antigen from Histoplasma capsulatum to a classic histoplasmin with the help of intradermal tests of delayed type of hypersensitivity. Tests were applied to 115 individuals in Santo Amaro, a town in the state of São Paulo. Positive results using classic histoplasmin were obtained in 46.0% cases whereas positive results using the polysaccharide antigen at its hightest concentration were obtained in 51.30% cases. The major conclusion in this investigation is that it is possible to use the polysaccharide antigen as histoplasmin instead of the filtrate antigenO estudo envolve a comparação entre o antígeno polissacarídico de Histoplasma capsulatum com a histoplasmina clássica em inquérito epidemiológico, através de provas intradérmicas de hipersensibilidade do tipo tardio, realizado em 115 indivíduos da região de Santo Amaro. Os resultados revelaram 46,0% de provas positivas com a histoplasmina clássica e 51,30% de resultados positivos com o antígeno polissacarídico em sua maior concentração. A principal conclusão da pesquisa: é possível utilizar o antígeno polissacarídico como histoplasmina, em substituição ao antígeno filtrado

  16. [Fungemia in hospitals of the city of Buenos Aires, Argentina].

    Science.gov (United States)

    López Moral, Laura; Tiraboschi, Iris Nora; Schijman, Mariela; Bianchi, Mario; Guelfand, Liliana; Cataldi, Silvana

    2012-01-01

    The incidence of fungi like pathogens in hospitals varies by regions. Our goal was not only to record the incidence and etiology of fungaemia, but also the change during the 4 years analysed, to determine the time of detection in automated blood culture and by lysis-centrifugation, and finally to assess the gender, age and underlying disease of the patients with fungaemia. An observational multicentre study of fungaemia was conducted in hospitals in the Mycology Network of Buenos Aires. A total of 190,920 blood cultures were processed: 182,050 automated blood culture and 8,870 lysis-centrifugation. Fungi were recovered in 1,020 episodes. The overall incidence of fungaemia was 1.72/1,000 admissions; 683 episodes were due to Candida (68%), and 325 (32%) to other fungi: 214 Cryptococcus, 105 Histoplasma, 7 Rhodotorula, 5 Trichosporon, 2 Pichia, 2 Acremonium, one Saccharomyces and one Fusarium. The incidence of candidaemia was 1.15/1,000 admissions with a wide variation between centres (0.35 to 2.65). Most Candida isolates (97%) were detected in the first 2 days of incubation. Candida albicans was recovered in 43% of the episodes. In fungaemia other than candidaemia, the predominant fungi were Cryptococcus and Histoplasma capsulatum. The incidence remained stable during the study period. Fungaemia by Candida were predominant. C. albicans was involved in less than a half of the episodes. The recovery of Cryptoccocus and H. capsulatum is strongly associated with HIV patients. Copyright © 2011 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.

  17. Diagnosis of histoplasmosis Diagnóstico laboratorial da histoplasmose

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    Allan Jefferson Guimarães

    2006-03-01

    Full Text Available Endemic mycoses can be challenging to diagnose and accurate interpretation of laboratory data is important to ensure the most appropriate treatment for the patients. Although the definitive diagnosis of histoplasmosis (HP, one of the most frequent endemic mycoses in the world, is achieved by direct diagnosis performed by micro and/or macroscopic observation of Histoplasma capsulatum (H. capsulatum, serologic evidence of this fungal infection is important since the isolation of the etiologic agents is time-consuming and insensitive. A variety of immunoassays have been used to detect specific antibodies to H. capsulatum. The most applied technique for antibody detection is immunodiffusion with sensitivity between 70 to 100 % and specificity of 100%, depending on the clinical form. The complement fixation (CF test, a methodology extensively used on the past, is less specific (60 to 90%. Detecting fungal antigens by immunoassays is valuable in immunocompromised individuals where such assays achieve positive predictive values of 96-98%. Most current tests in diagnostic laboratories still utilize unpurified antigenic complexes from either whole fungal cells or their culture filtrates. Emphasis has shifted, however, to clinical immunoassays using highly purified and well-characterized antigens including recombinant antigens. In this paper, we review the current conventional diagnostic tools, such as complement fixation and immunodiffusion, outline the development of novel diagnostic reagents and methods, and discuss their relative merits and disadvantages to the immunodiagnostic of this mycosis.As micoses endêmicas podem ser um desafio com relação ao diagnóstico e a interpretação acurada dos dados laboratoriais é importante para garantir um tratamento mais apropriado para os pacientes. Embora o diagnóstico definitivo da histoplasmose (HP, uma das micoses endêmicas no mundo, seja determinado pelo exame direto com a observação de micro e

  18. [Malian first observation of disseminated African histoplasmosis with predominant bone localizations in an HIV-negative child in Bamako (Mali). Review of the literature].

    Science.gov (United States)

    Minta, D K; Sylla, M; Traoré, A M; Soukho-Kaya, A; Coulibaly, I; Diallo, K; Théra, M A; Sidibé, A T; Sidibé, S; Traoré, H A; Pichard, E; Chabasse, D

    2014-06-01

    Endemic deep fungal infections are still under recognised diseases in daily medical practice because of their rarity in sub-Saharan area. The African histoplasmosis Histoplasma capsulatum var. duboisii (H. capsulatum duboisii) is the most frequent variety described in Mali through limited studies in adult patients, since the first case described by Catanei and Kervran (1945). Our case report is a disseminated histoplasmosis in a young 6-year-old african child. He was male and rural. The infectious localisations were mucosae, skin, lymphnodes, urinary tract and bones. Evolution has been marred by an episode of worsening of symptoms despite initial clinical improvement with ketoconazole. After healing of mucocutaneous lesions, we noticed a limitation of ampliation of both wrists. The radiographic bone lesions were lysis of the right lower end of the right radius and cubitus and fragmentation of cubital epiphysis of the same arm. Lacunes were present on the fifth right finger in metatarsus and phalanx; lacune and blowing aspect of the second phalanx of the left third finger was noted. The disseminated form of African histoplasmosis may occur in HIV-negative subject. The prognosis depends on early diagnosis and administration of appropriate and well-conducted therapy. Copyright © 2013. Published by Elsevier Masson SAS.

  19. Microbiology of systemic fungal infections

    Directory of Open Access Journals (Sweden)

    Chakrabarti A

    2005-01-01

    Full Text Available The increased incidence of systemic fungal infections in the past two decades has been overwhelming. Earlier, it was pathogenic dimorphic fungi, which were known to cause systemic infections. However, starting from the 1960s, opportunistic fungi started causing more number of infections, especially in the immunocompromised host. More recently, newer and less common fungal agents are being increasingly associated with infection in immunosuppressed hosts. Amongst dimorphic fungi, infections due to Histoplasma capsulatum and Penicillium marneffei are increasingly reported in patients with AIDS in India. H. capsulatum is found country wide, but P. marneffei remains restricted to Manipur state. Although both varieties of C. neoformans , C. neoformans var. neoformans (serotypes A & D, and C. neoformans var. gattii (serotypes B & C are reported in India, most of the cases reported are of serotype A. Increased incidence of cryptococcosis is reported from all centers with the emergence of AIDS. Systemic infection due to species under Candida , Aspergillus and zygomycetes is widely prevalent in nosocomial setting, and outbreaks due to unusual fungi are reported occasionally from tertiary care centers. This global change in systemic fungal infections has emphasized the need to develop good diagnostic mycology laboratories in this country and to recognize this increasingly large group of potential fungal pathogens.

  20. Defense reactions of Dermatobia hominis (Diptera: Cuterebridae) larval hemocytes.

    Science.gov (United States)

    Faraldo, Ana Carolina; Lello, Edy

    2003-08-01

    The defense reactions against biological (Histoplasma capsulatum and Escherichia coli) and non-biological materials (China ink and nylon thread) were tested in vivo in third instar larvae of Dermatobia hominis. The cellular defense performed by larval hemocytes was observed under electron microscopy. China ink particles were phagocytosed by granular cells 5 h after injection. E. coli cells were internalized by granular cells as early as 5 min after injection and totally cleared 180 min post-injection, when many hemocytes appeared disintegrated and others in process of recovering. H. capsulatum yeasts provoked, 24 h after being injected, the beginning of nodule formation. Nylon thread was encapsulated 24 h after the introduction into the hemocoel. Our results suggest that granular cells were the phagocytic cells and also the responsible for the triggering of nodule and capsule formation. In the presence of yeasts cells and nylon thread, they released their granules that chemotactically attracted the plasmatocytes that on their turn, flattened to surround and isolate the foreign material.

  1. Histoplasmose pulmonar canina no estado de Pernambuco, Brasil: relato de caso

    Directory of Open Access Journals (Sweden)

    T.I.B. Silva

    2013-12-01

    Full Text Available A histoplasmose é uma das principais doenças micóticas que acometem o trato respiratório inferior de pequenos animais, sendo ocasionada pelo Histoplasma capsulatum, fungo encontrado em solos ricos em compostos nitrogenados, derivados de matéria orgânica em decomposição. Descreve-se um caso de histoplasmose pulmonar em um canino da raça Boxer, domiciliado no estado de Pernambuco, Brasil, o qual apresentava uma síndrome respiratória com evolução clínica de aproximadamente seis meses. Na análise citopatológica do lavado broncoalveolar, foram visualizadas estruturas leveduriformes de aproximadamente dois micrômetros de diâmetro, características de H. capsulatum, sendo, então, indicada a terapia com itraconazol. O exame citopatológico do lavado broncoalveolar é uma ferramenta diagnóstica importante na identificação do agente, e o tratamento com itraconazol é eficiente, levando à remissão completa dos sinais clínicos.

  2. Inquérito soroepidemiológico para infecções por fungos causadores de micoses sistêmicas na Reserva Indígena Xacriabá, Estado de Minas Gerais

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    Martinez Roberto

    2002-01-01

    Full Text Available A Reserva Indígena Xacriabá situa-se no norte do Estado de Minas Gerais, próxima do município de Manga e do vale do Rio São Francisco. A população índia é miscigenada com brancos e negros e exerce atividades agrícolas e pecuária. Amostras de sangue foram coletadas de 180 habitantes da Reserva (85 homens e 95 mulheres, com 15 a 84 anos de idade, testando-se o soro pelo método da contraimunoeletroforese com antígenos de Paracoccidioides brasiliensis, Histoplasma capsulatum, Cryptococcus neoformans e Candida albicans. Verificou-se soros reagentes em, respectivamente, 5%, 3,9%, 7,2% e 6,7% do total de amostras. Os indivíduos reativos com antígenos de P. brasiliensis e H. capsulatum eram predominantemente mulheres e tinham idade menor e títulos médios mais elevados de anticorpos do que os reativos com outros antígenos.Os resultados são sugestivos da ocorrência de paracoccidiodomicose-infecção e de histoplasmose-infecção, nas primeiras décadas de vida dos habitantes, na área da Reserva Indígena Xacriabá.

  3. The Leukotriene B4/BLT1 Axis Is a Key Determinant in Susceptibility and Resistance to Histoplasmosis

    Science.gov (United States)

    Secatto, Adriana; Soares, Elyara Maria; Locachevic, Gisele Aparecida; Assis, Patricia Aparecida; Paula-Silva, Francisco Wanderlei Garcia; Serezani, Carlos Henrique; de Medeiros, Alexandra Ivo; Faccioli, Lúcia Helena

    2014-01-01

    The bioactive lipid mediator leukotriene B4 (LTB4) greatly enhances phagocyte antimicrobial functions against a myriad of pathogens. In murine histoplasmosis, inhibition of the LT-generating enzyme 5-lypoxigenase (5-LO) increases the susceptibility of the host to infection. In this study, we investigated whether murine resistance or susceptibility to Histoplasma capsulatum infection is associated with leukotriene production and an enhancement of in vivo and/or in vitro antimicrobial effector function. We show that susceptible C57BL/6 mice exhibit a higher fungal burden in the lung and spleen, increased mortality, lower expression levels of 5-LO and leukotriene B4 receptor 1 (BLT1) and decreased LTB4 production compared to the resistant 129/Sv mice. Moreover, we demonstrate that endogenous and exogenous LTs are required for the optimal phagocytosis of H. capsulatum by macrophages from both murine strains, although C57BL/6 macrophages are more sensitive to the effects of LTB4 than 129/Sv macrophages. Therefore, our results provide novel evidence that LTB4 production and BLT1 signaling are required for a histoplasmosis-resistant phenotype. PMID:24465479

  4. Extradural spinal cord lesion in a dog: first case study of canine neurological histoplasmosis in Italy.

    Science.gov (United States)

    Reginato, A; Giannuzzi, P; Ricciardi, M; De Simone, A; Sanguinetti, M; Porcellato, I; Mandara, M T

    2014-06-04

    A 7-year-old intact male mixed dog was presented with a history of acute and progressive paraparesis. Abnormal clinical signs consisted of non-ambulatory paraparesis, hind limbs hypertonia and severe thoracolumbar pain. Magnetic resonance imaging demonstrated an isointense in T1 and T2 WI epidural lesion, with good contrast enhancement, extending from T-10 to T-13. Laminectomy was carried out to remove the epidural mass. Histological examination revealed a pyogranulomatous lesion characterized by numerous macrophages containing yeast-like Grocott and PAS-positive bodies. Immunohistochemistry and PCR performed on formalin-fixed paraffin-embedded tissue confirmed Histoplasma capsulatum as the causative agent. H. capsulatum has a worldwide distribution in temperate and subtropical climates but its presence as an autochthonous fungus in Europe is now recognized. To the authors' knowledge this is the first report of canine histoplasmosis in Italy with lesion confined to the central nervous system. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Disseminated Histoplasmosis: A Challenging Differential Diagnostic Consideration for Suspected Malignant Lesions in the Digestive Tract

    Directory of Open Access Journals (Sweden)

    Bernhard Doleschal

    2016-11-01

    Full Text Available Histoplasmosis is well characterized as an endemic fungal disease restricted to certain areas of the USA. In Middle Europe, most patients present with acute pulmonary symptoms after travelling to endemic areas. Here, we want to illustrate the case of a 67-year-old man who presented with persistent oral ulcers, hoarseness, dysphagia, diarrhea, and weight loss to our Department of Otorhinolaryngology in December 2014. He was a retired construction worker and had a history of soil-disruptive activities in Africa and Middle and South America during employment. A positron emission tomography-computed tomography scan revealed prominent hypermetabolic lesions in the cecum and the lung, pointing towards a malignant disease. Surprisingly, histological examination of colonic and oral biopsies revealed abundant intracellular fungal elements, highly suspicious of Histoplasma capsulatum. Diagnosis was finally confirmed by panfungal polymerase chain reaction. Upon treatment with liposomal amphotericin followed by itraconazole, the severely ill patient showed an impressive clinical response. This case describes a disseminated manifestation of H. capsulatum years after the first exposure in an otherwise immunocompetent patient descending from a nonendemic area.

  6. Histoplasmosis diseminada, linfopenia, y síndrome de Sjögren

    Directory of Open Access Journals (Sweden)

    Horacio F. Rodrigo

    2012-10-01

    Full Text Available El síndrome de Sjögren es una enfermedad autoinmune caracterizada por disminución de las secreciones de las glándulas exocrinas; puede presentar también diversas alteraciones hemáticas, entre ellas linfopenia. Presentamos el caso de un varón de 28 años que consultó por cefalea de un mes de evolución a la que se agregaron en las últimas 48 horas vómitos y fiebre. Presentaba lesiones en piel de tronco y cara; no tenía rigidez de nuca. Se demostró infección por Histoplasma capsulatum var. capsulatum en piel y líquido cefalorraquídeo, linfopenia, anticuerpos anti Ro-SSA positivos, baja concentración del trazador en centellograma de glándulas salivales e infiltración linfocitaria en glándulas salivales, lo que permitió confirmar al diagnóstico de síndrome de Sjögren. El tratamiento con anfotericina liposomal e itraconazol mejoró el cuadro clínico. Comunicamos este caso para referir que una infección oportunista, como la histoplasmosis diseminada, puede ser una forma poco común de presentación del síndrome de Sjögren.

  7. Brote de histoplasmosis aguda en un grupo familiar: identificación de la fuente de infección

    Directory of Open Access Journals (Sweden)

    Roberto Alonso Jiménez

    2002-06-01

    Full Text Available Se informa un brote de histoplasmosis ocurrido en los integrantes de una familia y que comprometió a cuatro personas, dos mujeres, una niña y un hombre. El caso índice consultó por sintomatología respiratoria grave, de comienzo súbito, que requirió hospitalización. En los otros casos, la infección cursó de manera asintomática pero se puso en evidencia por la reactividad en las pruebas serológicas con histoplasmina. La búsqueda de una fuente común de contagio llevó a sospechar que era la tierra de un vivero que se había utilizado como fertilizante de las plantas caseras. Las suspensiones de las tierras de las macetas sirvieron para inocular ratones BALB/c, de cuyos órganos fue posible aislar el agente etiológico, Histoplasma capsulatum var. capsulatum. Si bien la histoplasmosis es más frecuente en ciertas ocupaciones y es propia de áreas rurales, las epidemias y los brotes son ahora comunes en áreas urbanas debido a actividades como la urbanización masiva, la tala de árboles, las demoliciones y el uso de tierras enriquecidas con abonos orgánicos (gallinaza, guano. Se llama la atención sobre el peligro que representa esta última actividad.

  8. Proposed additions to the National Wilderness Preservation System : Part 20 : J. N. "Ding" Darling Wilderness

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document is one of the Presidential Transmittals proposing additions to the National Wilderness Preservation System. This particular transmittal focuses on the...

  9. Mercury Concentrations in Biota from J.N. "Ding" Darling National Wildlife Refuge: A Baseline Study

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — In 1990, Lee County, Florida planned to construct a combination solid waste incinerator/power generating plant. Solid waste incinerators in other areas of the United...

  10. J.N. "Ding" Darling National Wildlife Refuge: A Report on Wilderness Character Monitoring

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document is the completed effort of the U.S. Fish and Wildlife Service, Wilderness Fellows program to develop a monitoring strategy and evaluate the status of...

  11. 75 FR 27576 - J.N. “Ding” Darling National Wildlife Refuge, Lee County, FL

    Science.gov (United States)

    2010-05-17

    ... surveying and monitoring activities and increased water management capabilities for the impoundments, the.... Ehrhardt, Natural Resource Planner, telephone: 321/861-2368; or Mr. Paul Tritaik, Refuge Manager, telephone... protection of important resources; (6) declines in and threats to rare, threatened, and endangered species...

  12. Cultural Resources Survey for the Lake Darling-Souris River Project, North Dakota (1982).

    Science.gov (United States)

    1985-06-13

    finishings on pottery , the Saskatchewan Basin sequence known from the Morkin Site, and the Cluny Complex, a proto- historic manifestation identified at...the Cluny Site in Alberta. Pottery found on the Souris River may be related to the Late Woodland ceramic-making cultures of seuthern Canada (see... Pottery and Projectile Points (site lead) Schweigert, 1978 " (SW SE ) 32RV435 Servold Homestead House Schweigert, 1978 Sec. 17 (SW NWh) McCarroll Ranch

  13. Lake Darling Flood Control Project, Souris River, North Dakota. General Project Design.

    Science.gov (United States)

    1983-06-01

    rhaa iorkl a onia rnahan atoloeasl so.ropho clatk "I..o afag. - W5 1talas 1 L lossl oss t slanlot Snort--p aiana o iapatt todad diaifianasa nold itr...R/,/66 /o PLT M0.5 EMIL / P * END LEVEE / STAION 51+15 STATION 50+00 TIO 10+0 9 00 \\0 GRA/E L FILL FOR ID.RVE*WWTTINiO O j REMOVE? P-, STE~ FILLI TO

  14. How to put your darlings on a diet. Philosophical practice as an epistemological practice

    DEFF Research Database (Denmark)

    Lystbæk, Christian Tang

    and critical attitude towards knowledge in health care practice. Drawing on the concept of philosophy as a way of “situating reason” (McCumber, 2005, a.o.), this paper explores the potential of Philosophical Practice with regards to opening up common conceptions of “knowledge management” in health care......, such as the Cochrane Collaborations. Thus, science-generated knowledge and its practices are spreading to social and professional life more generally. These developments commonly invite health care professionals to participate in a range of practices in which knowledge itself becomes an issue. It is not only knowledge...... that is spreading, but also ways of engaging with knowledge, historically associated with research communities. This contribution to the 5th Nordic Conference on Philosophical Practice describes how Philosophical Practice can enter the epistemic practices in health care organizations by inviting an open...

  15. Mercury in fishes of the J.N. "Ding" Darling National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From June 26 to 30,1990 spotted gar (Lepisosteus oculatus), spotted seatrout (Cynoscion nebulosus), gafftopsail catfish (Bagre marinus), and largemouth bass...

  16. Who is Everyone's Darling in Cyberspace? The Characteristics of Popular Online Daters

    Directory of Open Access Journals (Sweden)

    Chih-Chien Wang

    2010-06-01

    Full Text Available Online dating current is a popular activity in cyberspace. More and more people make new friends and find their partners through online dating web sites. There is an interesting observation that some people are more popular than others in online dating web sites. The current study focuses on the personal profile characteristics that make one a popular dater. By two field surveys, this study discusses the relationship between online daters' personal profiles and their popularity. The first survey investigated 800 online daters' profiles from dating web sites. The statistical analysis results indicated thatphysical body types and looks, education level, occupation, personality, and interests were characteristics that influenced the popularity of online daters, while significant gender differences were found in the characteristics that related to popularity of online daters. The second survey searched 960 online daters with profiles of popular and unpopular characteristics. By observing the popularity of these online daters, it was found that those with "popular" characteristics also were the popular daters and those with "unpopular" characteristics were the unpopular daters. This may serve as evidence that the found personal profile characteristics were indeed factors influencing the popularity of the online daters.

  17. Variation in size-structure, diet and habitat selection in the Darling hardyhead, Craterocephalus amniculus

    DEFF Research Database (Denmark)

    2017-01-01

    or cobble substrate, increased channel depth and width and distance from the bank, and reduced flow velocity. Exotic riparian vegetation cover was avoided, and in-stream woody debris was underutilised. This species may be vulnerable to further population decline in light of its habitat preferences...

  18. Acidification and buffering mechanisms in acid sulfate soil wetlands of the Murray-Darling Basin, Australia.

    Science.gov (United States)

    Glover, Fiona; Whitworth, Kerry L; Kappen, Peter; Baldwin, Darren S; Rees, Gavin N; Webb, John A; Silvester, Ewen

    2011-04-01

    The acid generation mechanisms and neutralizing capacities of sulfidic sediments from two inland wetlands have been studied in order to understand the response of these types of systems to drying events. The two systems show vastly different responses to oxidation, with one (Bottle Bend (BB) lagoon) having virtually no acid neutralizing capacity (ANC) and the other (Psyche Bend (PB) lagoon) an ANC that is an order of magnitude greater than the acid generation potential. While BB strongly acidifies during oxidation the free acid generation is less than that expected from the measured proton production and consumption processes, with additional proton consumption attributed to the formation of an acid-anion (chloride) FeIII (oxyhydr)oxide product, similar to akaganéite (Fe(OH)2.7Cl0.3). While such products can partially attenuate the acidification of these systems, resilience to acidification is primarily imparted by sediment ANC.

  19. The Road Inventory of J.N. Ding Darling National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To determine the relative needs of the U.S. Fish and Wildlife Service, the Federal Highway Administration (FHWA) was asked to inventory all public access and...

  20. Case reports 1964 - pathology. Histoplasmosis of the kidney

    Energy Technology Data Exchange (ETDEWEB)

    Will, D.W.; Hasegawa, C.M.; Kawauchi, Tadanaga; Murashima, Shoichi

    1964-07-16

    A case is presented of isolated renal histoplasmosis in a Nagasaki woman who died of pulmonary tuberculosis following massive x-ray therapy for squamous carcinoma of the mouth. No primary pulmonary lesion was identified. While the patient's history includes a 40-year residence in Malaysia and a brief internment in New Delhi where a few cases of histoplasmosis have been reported, she had been in Japan for 16 years prior to her death. In view of the presently-known absence of viability of Histoplasma in old primary foci, it was concluded that the infection was acquired in Japan and represents the first case of Histoplasma capsulatum infection reported from Japan (37 references, 5 figures). A second case is presented of a 15-year-old Japanese girl with a clinical history and postmortem findings of a subacute necrotizing encephalomyelopathy is reported. The patient's parents were consanguinous and a similar disease was also present in one sibling. This case and those available in the literature indicate that a severe peripheral neuropathy associated frequently with secondary degeneration of the dorsal white columns of the spinal cord constitutes an integral part of the disorder. The presence of peripheral neuropathy reminiscent of that due to deficiency of thiamine and pantothenic acid, frequent familial occurrence of the disorder and consanguinity in some of the parents give support to the speculation of previous investigators that the disease may represent an inborn error of metabolism especially of pyruvate metabolism. 11 references, 15 figures, 1 table.

  1. Endemic mycoses: a treatment update.

    Science.gov (United States)

    Lortholary, O; Denning, D W; Dupont, B

    1999-03-01

    Endemic mycoses remain a major public health problem in several countries and they are becoming increasingly frequent with the spread of HIV infection. Amphotericin B remains the drug of choice during the acute stage of life-threatening endemic mycoses occurring in both immunocompetent and immunocompromised hosts. Ketoconazole is effective in non-AIDS patients with non-life-threatening histoplasmosis, blastomycosis, or paracoccidioidomycosis. Itraconazole is the treatment of choice for non-life-threatening Histoplasma capsulatum or Blastomyces dermatitidis infections occurring in immunocompetent individuals and is the most efficient secondary prophylaxis of histoplasmosis in AIDS patients. Itraconazole is also effective in lymphocutaneous and visceral sporotrichosis, in paracoccidioidomycosis, for Penicillum marneffei infection, and is an alternative to amphotericin B for Histoplasma duboisii infection. Coccidioidomycosis may be effectively treated with prolonged and sometimes life-long itraconazole or fluconazole therapy. Fluconazole has relatively poor efficacy against histoplasmosis, blastomycosis and sporotrichosis. New antifungal agents have been tested in vitro or in animal models and may soon be evaluated in clinical trials.

  2. Generation of IL-23 Producing Dendritic Cells (DCs) by Airborne Fungi Regulates Fungal Pathogenicity via the Induction of TH-17 Responses

    Science.gov (United States)

    Chamilos, Georgios; Ganguly, Dipyaman; Lande, Roberto; Gregorio, Josh; Meller, Stephan; Goldman, William E.; Gilliet, Michel; Kontoyiannis, Dimitrios P.

    2010-01-01

    Interleukin-17 (IL-17) producing T helper cells (TH-17) comprise a newly recognized T cell subset with an emerging role in adaptive immunity to a variety of fungi. Whether different airborne fungi trigger a common signaling pathway for TH-17 induction, and whether this ability is related to the inherent pathogenic behavior of each fungus is currently unknown. Here we show that, as opposed to primary pathogenic fungi (Histoplasma capsulatum), opportunistic fungal pathogens (Aspergillus and Rhizopus) trigger a common innate sensing pathway in human dendritic cells (DCs) that results in robust production of IL-23 and drives TH-17 responses. This response requires activation of dectin-1 by the fungal cell wall polysaccharide b-glucan that is selectively exposed during the invasive growth of opportunistic fungi. Notably, unmasking of b-glucan in the cell wall of a mutant of Histoplasma not only abrogates the pathogenicity of this fungus, but also triggers the induction of IL-23 producing DCs. Thus, b-glucan exposure in the fungal cell wall is essential for the induction of IL-23/TH-17 axis and may represent a key factor that regulates protective immunity to opportunistic but not pathogenic fungi. PMID:20886035

  3. HEPATITIS AND PNEUMONITIS DURIN ADALIMUMAB THERAPY IN CROHN? DISEASE: mind the histoplasmosis!

    Directory of Open Access Journals (Sweden)

    Bruno do Valle PINHEIRO

    2014-03-01

    Full Text Available Context Tumor necrosis factor-alpha (TNF-α inhibitor therapy plays a pivotal role in the management of moderate to severe inflammatory bowel disease. Because of the role of TNF-α in the host defenses, anti-TNF therapy has been associated with an increase the risks of granulomatous infections. Objective To report the first case of adalimumab-associated invasive histoplasmosis presenting as an acute hepatitis-like syndrome and febrile pneumonitis in a patient with Crohn’s disease. Method Case report of a patient with progressive histoplasmosis confirmed by percutaneous fine needle aspiration biopsy lung and urine Histoplasma antigen. Results We present the case of a young man with CD who developed pneumonia and acute hepatitis-like features caused by Histoplasma capsulatum infection during adalimumab therapy. To the best of our knowledge, this acute hepatitis-like manifestation has never been reported as a presentation of the histoplasmosis in patients with Crohn’s disease. Conclusions This case underscores the potential risk for serious infection that may arise in this setting and should alert clinicians to the need to consider the histoplasmosis diagnosis in patients presenting with acute hepatitis-like syndrome associated with prolonged febrile illness or pneumonitis during therapy with anti-TNF-α antibodies.

  4. Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi.

    Science.gov (United States)

    Brilhante, Raimunda Sâmia Nogueira; Caetano, Érica Pacheco; Lima, Rita Amanda Chaves de; Marques, Francisca Jakelyne de Farias; Castelo-Branco, Débora de Souza Collares Maia; Melo, Charlline Vládia Silva de; Guedes, Glaucia Morgana de Melo; Oliveira, Jonathas Sales de; Camargo, Zoilo Pires de; Moreira, José Luciano Bezerra; Monteiro, André Jalles; Bandeira, Tereza de Jesus Pinheiro Gomes; Cordeiro, Rossana de Aguiar; Rocha, Marcos Fábio Gadelha; Sidrim, José Júlio Costa

    This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains of Coccidioides posadasii in filamentous phase (n=22) and Histoplasma capsulatum in both filamentous (n=40) and yeast phases (n=13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720μg/mL, 20-2860μg/mL, and 40-1420μg/mL, respectively for terpinen-4-ol; 250-4000μg/mL, 30-2000μg/mL, and 10-1000μg/mL, respectively, for tyrosol; and 0.48-7.8μg/mL, 0.25-16μg/mL, and 0.125-4μg/mL, respectively for β-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  5. Histoplasma-associated inflammatory pseudotumour of the kidney mimicking renal carcinoma

    NARCIS (Netherlands)

    M.A. den Bakker (Michael); N.N.T. Goemaere (Natascha); J.A. Severin (Juliëtte); J.L. Nouwen (Jan); P.C.M.S. Verhagen (Paul)

    2009-01-01

    textabstractA 56-year-old female, originally from Suriname, with an otherwise unremarkable previous medical history was found to have a renal mass highly suspicious for renal cancer for which a nephrectomy was performed. Within the kidney, a tumourous mass was found which, on histological

  6. Histoplasma com derrame pleural: relato de um caso Histoplasmoma and pleural effusion: a case report

    Directory of Open Access Journals (Sweden)

    Marcos C. de Almeida

    1990-10-01

    Full Text Available Efusões pleurais surgem raramente em associação com a histoplasmose capsu-lata, ocorrendo em geral nas formas agudas da doença. Relatamos e discutimos um caso clínico em que um histoplasmoma subpleural acompanhou-se de dor pleurítica, hidrotórax e pleurite fibrosante.

  7. Histoplasmose pulmonar aguda no Rio Grande do Sul Acute pulmonary histoplasmosis in the State of Rio Grande do Sul, Brazil

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    Gisela Unis

    2005-02-01

    Full Text Available INTRODUÇÃO: A histoplasmose pulmonar aguda depende da inalação de uma grande quantidade de propágulos fúngicos por um paciente hígido. O tempo de exposição determina a gravidade da doença. Uma epidemia é influenciada por fatores que afetam o crescimento e a transmissão do Histoplasma capsulatum var. capsulatum na natureza. OBJETIVO: Identificar os aspectos epidemiológicos e clínico-laboratoriais dos pacientes com histoplasmose pulmonar aguda no Rio Grande do Sul e compará-los com as microepidemias relatadas no Brasil. MÉTODO: Foram revisados 212 prontuários clínicos de pacientes com histoplasmose dos arquivos do Laboratório de Micologia do Complexo Hospitalar Santa Casa de Porto Alegre (RS num período de 25 anos (1977-2002. Foram identificados e incluídos no estudo os casos de histoplasmose pulmonar aguda com cultivo positivo e/ou achado histopatológico compatível. As microepidemias foram diagnosticadas com a comprovação de um caso ou evidência soromicológica com história clínica compatível. Foram revisadas as microepidemias publicadas no Brasil. RESULTADOS: Dezoito de um total de 212 pacientes (8,5% foram incluídos no trabalho. A idade variou de 8 a 63 anos (média de 35,4; mediana de 34,5, e 67% eram do sexo masculino. A história epidemiológica foi sugestiva em 11 pacientes (61%. O tipo primário de histoplasmose pulmonar aguda foi o mais freqüente (17; 95%. Houve predomínio de casos isolados. CONCLUSÃO: O reconhecimento de casos isolados e a presença de microepidemias demonstram a abundância do H. capsulatum no solo, e juntamente com a ocorrência de todas as formas da doença, confirmam o Rio Grande do Sul como hiperendêmico para histoplasmose.BACKGROUND: Acute pulmonary histoplasmosis is a respiratory infection occurring when an otherwise healthy individual inhales a large quantity of fungal propagules. Length of exposure determines disease severity. An epidemic is influenced by factors affecting the

  8. HISTOPLASMOSE AFRICAINE DISSEMINEE CHEZ UN ENFANT IMMUNOCOMPETENT AU BURKINA FASO: UN CAS [DISSEMINATED AFRICAN HISTOPLASMOSIS IN AN IMMUNOCOMPETENT CHILD IN BURKINA FASO: ONE CASE

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    Gilles Lemasson

    2013-07-01

    Full Text Available Résumé Introduction: L’histoplasmose à Histoplasma capsulatum var. duboisii est une affection rare en Afrique. Les lésions cutanées localisées sont les plus fréquentes. Nous rapportons une forme disséminée chez un enfant immunocompétent. Observation: Une élève de 8 ans est hospitalisée pour des nodules cutanés, généralisés, associés à des douleurs ostéo-articulaires très intenses de la quasi-totalité des articulations. L’examen a noté une malnutrition aiguë modérée, des nodules sous cutanés, multiples, des papules rosées, à surface plane, des papules ombiliquées, des tuméfactions nodulaires très douloureuses de plusieurs articulations, de multiples adénopathies fermes, mobiles, une atteinte osseuse multiple à la radiographie, une hépatosplénomégalie et des ulcérations secondaires douloureuses. L’histologie d’un nodule cutané et d’une papule ombiliquée a mis en évidence H.capsulatum var. duboisii. Après l’échec d’un traitement au fluconazole, l’évolution a été favorable sous l’amphotéricine B. La patiente a bénéficié de la collaboration Nord-Sud et de l’aide des structures sociales.Conclusion: Cette observation nous a permis de décrire les particularités cliniques et socio-économiques, les difficultés diagnostiques et thérapeutiques d’un cas d’histoplasmose africaine disséminée et de démontrer encore l’efficacité de l’amphotéricine B. AbstractIntroduction: Histoplasmosis due to Histoplasma capsulatum var. duboisii is a rare affection in Africa. Localized cutaneous lesions are the most common form. We report a disseminated form in an immunocompetent child. Case report: An 8-year-old student has been hospitalized for generalized, cutaneous nodules associated with very severe osteo-articular pains of almost all the joints. The examination has noted a moderate acute malnutrition, multiple and sub-cutaneous nodules, pinkish and plan papules, umbilicate papules

  9. Micoses sistêmicas: fatores associados ao óbito em pacientes com infecção pelo vírus da imunodeficiência humana, Cuiabá, Estado de Mato Grosso, 2005-2008 Systemic mycosis: factors associated with death among patients infected with the human immunodeficiency virus, Cuiabá, State of Mato Grosso, Brazil, 2005-2008

    Directory of Open Access Journals (Sweden)

    Luciano Correa Ribeiro

    2009-12-01

    Full Text Available A prevalência de micose sistêmica entre 1.300 pacientes portadores de HIV/Aids de Cuiabá, Mato Grosso foi de 4,6%, no período de 2005-2008. As espécies de fungos isoladas foram o Cryptococcus neoformans (50%, Cryptococcus gattii (1,6%, Cryptococcus spp (6,6%, Histoplasma capsulatum (38,3% e Paracoccidioides brasiliensis (3,3%. Óbito foi registrado em 32 (53,3% pacientes, sendo a criptococose a principal causa. A contagem de linfócitos T CD4+ foi baixa e semelhante entre os pacientes que sobreviveram ou faleceram por micose sistêmica. O etilismo (OR:8,2; IC95%: 1,4-62,1; p=0,005 e o nível médio de desidrogenase lática [758 (182 U/L vs 416 (268 U/L; pBetween 2005 and 2008, the prevalence of systemic mycosis among 1,300 HIV/AIDS patients in Cuiabá, Mato Grosso, was 4.6%. The fungus species isolated were Cryptococcus neoformans in 50%, Cryptococcus gattii in 1.6%, Cryptococcus spp in 6.6%, Histoplasma capsulatum in 38.3% and Paracoccidioides brasiliensis in 3.3%. Death was recorded in the cases of 32 patients (53.3%, and cryptococcosis was the main cause. The CD4+ T lymphocyte count was low and similar among patients who survived or died due to systemic mycosis. The factors independently associated with the deaths of these patients were alcoholism (OR: 8.2; 95% CI: 1.4-62.1; p = 0005 and the mean level of lactate dehydrogenase [758 (182 U/l vs. 416 (268 U/l; p < 0001]. The findings showed that systemic mycosis was highly lethal among the patients with HIV/AIDS in Cuiabá and suggested that clinical-laboratory characteristics such as alcoholism and early elevation of lactate dehydrogenase may be factors relating to worse prognosis under these conditions.

  10. Frecuencia de micosis en pacientes inmunosuprimidos de un hospital regional de la Ciudad de México Frequency of mycoses in immunosuppressed patients in a regional hospital in Mexico City

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    Francisca Hernández-Hernández

    2003-12-01

    Full Text Available OBJETIVO: Aislar e identificar hongos en diferentes especímenes de pacientes inmunocomprometidos, atendidos en un hospital de la Ciudad de México, y determinar su asociación con micosis. MATERIAL Y MÉTODOS: Se realizó un estudio de tipo observacional transversal en pacientes del Hospital Regional General Ignacio Zaragoza, de junio de 1999 a mayo de 2000. De 108 pacientes se procesaron 268 especímenes para estudio micológico que incluyó examen directo, frotis, cultivos y microcultivos en medios específicos además de pruebas bioquímicas. La mayoría de pacientes tenía diagnóstico clínico de tuberculosis pulmonar y de síndrome de inmunodeficiencia adquirida. RESULTADOS: Se obtuvieron 183 aislamientos de levaduras y 66 de hongos filamentosos. Se diagnosticaron 45 micosis que en su mayoría correspondieron a candidosis pulmonar (32 casos. Las especies de Candida más frecuentes asociadas a patología fueron Candida albicans y C parapsilosis. Se obtuvieron cinco aislamientos de Cryptococcus neoformans variedad neoformans, uno de C albidus, tres de Histoplasma capsulatum y uno de Geotrichum candidum, asociados a infección micótica. CONCLUSIONES: El 41.6% de los pacientes estudiados presentaron una micosis asociada principalmente a síndrome de inmunodeficiencia adquirida y a tuberculosis pulmonar.OBJECTIVE: To isolate and identify the fungi in specimens collected from immunocompromised patients seen in Mexico City hospital, and to assess their association with mycosis. MATERIAL AND METHODS: A total of 268 specimens from 108 patients were processed for mycological study, including direct examination, smears, cultures, and microcultures in specific media, in addition to biochemical tests. Most of the patients had been diagnosed with pulmonary tuberculosis and Acquired Immunodeficiency Syndrome (AIDS. RESULTS: One hundred and eighty-three yeasts and 66 mycelial fungi were isolated. Forty-five mycoses were diagnosed; the most frequent

  11. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen.

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    Sinem Beyhan

    2013-07-01

    Full Text Available Survival at host temperature is a critical trait for pathogenic microbes of humans. Thermally dimorphic fungal pathogens, including Histoplasma capsulatum, are soil fungi that undergo dramatic changes in cell shape and virulence gene expression in response to host temperature. How these organisms link changes in temperature to both morphologic development and expression of virulence traits is unknown. Here we elucidate a temperature-responsive transcriptional network in H. capsulatum, which switches from a filamentous form in the environment to a pathogenic yeast form at body temperature. The circuit is driven by three highly conserved factors, Ryp1, Ryp2, and Ryp3, that are required for yeast-phase growth at 37°C. Ryp factors belong to distinct families of proteins that control developmental transitions in fungi: Ryp1 is a member of the WOPR family of transcription factors, and Ryp2 and Ryp3 are both members of the Velvet family of proteins whose molecular function is unknown. Here we provide the first evidence that these WOPR and Velvet proteins interact, and that Velvet proteins associate with DNA to drive gene expression. Using genome-wide chromatin immunoprecipitation studies, we determine that Ryp1, Ryp2, and Ryp3 associate with a large common set of genomic loci that includes known virulence genes, indicating that the Ryp factors directly control genes required for pathogenicity in addition to their role in regulating cell morphology. We further dissect the Ryp regulatory circuit by determining that a fourth transcription factor, which we name Ryp4, is required for yeast-phase growth and gene expression, associates with DNA, and displays interdependent regulation with Ryp1, Ryp2, and Ryp3. Finally, we define cis-acting motifs that recruit the Ryp factors to their interwoven network of temperature-responsive target genes. Taken together, our results reveal a positive feedback circuit that directs a broad transcriptional switch between

  12. Disseminated histoplasmosis in patients with AIDS. Buenos Aires, 2009-2014

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    Adriana G López Daneri

    2016-12-01

    Full Text Available A retrospective study was carried out on 171 cases of disseminated histoplasmosis diagnosed in HIV/AIDS patients during the period 2009-2014. Although HIV diagnosis rates remained stable over the study period, a sensible increase in the number of histoplasmosis cases was observed in the last three years. Disseminated histoplasmosis was prevalent in males with an average age of 37.8 years. At diagnosis, only 54/171 (31.6% were receiving HAART, and CD4+ T-lymphocyte counts ranged from 4 to 264 cells/upsilon. Cutaneous lesions, including ulcerated papules or molluscoid plaques, were present in 110/171 (64.3%, with Histoplasma capsulatum being observed in all skin scraping specimens upon Giemsa staining. Respiratory manifestations were second in frequency with bronchoalveolar lavage showing a high diagnostic performance. Radiological findings included milliary patterns, interstitial infiltrates, and focalized condensations. Out of 141 blood cultures performed, H. capsulatum was isolated in 82 (58.2%. No significant difference in diagnostic performance was found between blood cultures and skin scraping (p = 0.6164. Other opportunistic infections were observed in 70/171 (40.9% prior to or concomitantly with histoplasmosis. Association with Mycobacterium tuberculosis was recorded in 16/171 (9.4% and one had a multi-drug resistant isolate. The severity of histoplasmosis determined the monotherapy with amphotericin B deoxycholate in 115 (67.3%, itraconazole in 42 (24.5%, and combined therapies in 14 (8.2%. Mortality was 19.9% (34/171. Finally, we emphasize that the higher prevalence in the last three years of the study should prompt the medical community to consider the diagnosis of histoplasmosis to reduce mortality of AIDS patients.

  13. Using mental-modelling to explore how irrigators in the Murray–Darling Basin make water-use decisions

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    Ellen M. Douglas

    2016-06-01

    New hydrological insights for the region: Results suggest support for greater local and irrigator involvement in water management decisions. Many, if not most, of the irrigators understood the need for, or at least the inevitability of, governmental policies and regulations. However, a lack of accountability, predictability, and transparency has added to the uncertainty in farm-based water decision-making. Irrigators supported the concept of environmental sustainability, although they might not always agree with how the concept is implemented. The mental modelling approach facilitated knowledge sharing among stakeholders and can be used to identify common goals. Future research utilizing the mental modelling approach may encourage co-management and knowledge partnerships between irrigators, water managers and government officials.

  14. An Archaeological Survey: Shoreline of Lake Darling and Proposed Burlington Dam. Flood Control Project Area, Upper Souris River, North Dakota

    Science.gov (United States)

    1978-01-01

    Sakakawea (Garrison Reservoir) by Haberman and Schneider (1975) and Leaf (1976), surveys in Dunn and Mercer counties (Fox et al. 1976; Lawrence Loendorf...Department of Botony, North Dakota State University, Fargo. Leaf, Gary 1976 Shoreline Survey of Lake Sakakawea : The Badlands in Southern Dunn County, North

  15. The transformation of organic carbon during river-groundwater exchange: An example from the Murray-Darling Basin

    Science.gov (United States)

    Keshavarzi, M.; Baker, A.; Andersen, M. S.; Kelly, B. F. J.

    2016-12-01

    Groundwater systems connected to rivers can act as carbon sinks and sources, but little is known about the distribution, transformation, and retention of organic carbon in rivers connected to aquifers as few studies are available. The characterisation of dissolved organic matter (DOM) using optical absorbance in connected water systems has potential to provide novel insights about the organic component of carbon fluxes. Here, the optical absorbance of the river and groundwater samples is investigated in a river reach that is hydraulically connected to an adjoining alluvial and karst aquifer system, within a semi-arid agricultural catchment in New South Wales, Australia. Water samples were collected from the river and groundwater within monitoring boreholes and intercepted by caves. These water samples were analysed for absorbance, dissolved organic carbon (DOC) and inorganic chemical constituents. Groundwater samples collected close to the river have DOM characteristics similar to the river water, indicating losing conditions. While, groundwater samples collected further away from the river have lower DOC and absorbance, higher SUVA, and a lower and more variable spectral slope, compared to the river. We infer that this change in DOM character reveals the presence of sedimentary OM, which provides a source of relatively high molecular weight DOM that is subsequently transformed. In a dry period, when there was low flow in the river, three downstream river-water samples exhibited low absorbance and spectral slope similar to the groundwater, while the contemporaneous upstream river-water samples had higher absorbance and spectral slope. This suggests gaining conditions and a contribution of groundwater organic carbon into the river. It is concluded that optical analyses can be used to study organic carbon fluxes to differentiate and quantify the source of organic matter, and identify losing and gaining streams.

  16. Brote de histoplasmosis en la Escuela de Cadetes de la Base Aérea de Morón, Provincia de Buenos Aires, República Argentina Histoplasmosis outbreak in Morón, Buenos Aires Province, Argentina

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    R. Negroni

    2010-12-01

    Full Text Available Se describe un brote de histoplasmosis que afectó a 6 cadetes de la Fuerza Aérea Argentina, sin antecedentes patológicos previos. Todos consultaron por problemas respiratorios después de haber limpiado un hangar. En ese recinto se encontraron abundantes deyecciones de animales, presuntamente de palomas y murciélagos. Los pacientes sufrieron fiebre, mialgias, taquipnea y tos no productiva. Las radiografías y tomografías de tórax mostraron imágenes pulmonares micronodulares, engrosamiento de los tabiques interalveolares y adenopatías hiliares. Todos tuvieron una evolución favorable y no requirieron tratamiento antifúngico. Las pruebas de inmunodifusión y contrainmunoelectroforesis con antígenos de Histoplasma capsulatum fueron positivas, al igual que las intradermorreacciones con histoplasmina. Se recogieron 5 muestras de tierra del lugar, las que fueron inoculadas por vía intraperitoneal a 20 hámsteres. De los cultivos de hígado y bazo de dichos animales se consiguió aislar la fase micelial de H. capsulatum. La cepa aislada se comparó con las obtenidas de 12 pacientes argentinos utilizando perfiles genéticos y se observó un clado único con más de 96% de similitud, lo que confirma la homogeneidad de las cepas argentinas. Si bien la histoplasmosis es endémica en la Pampa húmeda, este es el primer brote totalmente documentado al sur del paralelo 34°.An histoplasmosis outbreak affecting 6 previously healthy Air Force cadets is herein presented. The patients suffered from fever and respiratory symptoms after having cleaned an abandoned hangar soiled with pigeons and bat droppings. They all presented fever, myalgia, tachypnea, and nonproductive cough. Chest X-ray and CT scan studies showed disseminated reticulonodular images affecting both lungs. Hiliar adenomegalies were also observed. All patients achieved a favourable outcome without antifungal treatment. Both serologic tests searching for specificic antibodies

  17. [Infections in the child with acute leukemia].

    Science.gov (United States)

    Carrillo, J M; Jiménez, E; Jiménez, R

    1981-01-01

    One hundred and twenty-five febrile episodes in 82 children with acute leukemia were studied; 46% of the patients were from urban and 54% from rural areas. The origin of the fever was identified in 91% of the episodes, prevailing pneumonia, septicemia, chickenpox and herpes zoster. The etiological agent was identified in 46% of the cases. A viral predominance was evident, and among them varicela-zoster, following in importance gram-negative bacteria. Histoplasma capsulatum and Pneumocystis carinii were isolated in two occassions each. Sepsis was found more frequently in children with active leukemia than in those in remission (p less than 0.001). Forty-four febrile episodes occurred in patients with less than 1,000 neutrophils/ul. The daily-risk rate of infection was higher in children fom rural than in those from urban areas (p less than 0.001). After clinical and laboratory studies, methicillin and gentamicin were used, in addition to carbenicillin or trimethoprim-sulfamethoxazole is selected cases. This treatment was effective in 86% of the cases. Twelve (15%) children died, 6 of whom were in remission at that moment.

  18. Anti-inflammatory, analgesic and anti-oedematous effects of Lafoensia pacari extract and ellagic acid.

    Science.gov (United States)

    Rogerio, Alexandre P; Fontanari, Caroline; Melo, Mirian C C; Ambrosio, Sérgio R; de Souza, Glória E P; Pereira, Paulo S; França, Suzelei C; da Costa, Fernando B; Albuquerque, Deijanira A; Faccioli, Lúcia H

    2006-09-01

    Lafoensia pacari St. Hil. (Lythraceae) is used in traditional medicine to treat inflammation. Previously, we demonstrated the anti-inflammatory effect that the ethanolic extract of L. pacari has in Toxocara canis infection (a model of systemic eosinophilia). In this study, we tested the anti-inflammatory activity of the same L. pacari extract in mice injected intraperitoneally with beta-glucan present in fraction 1 (F1) of the Histoplasma capsulatum cell wall (a model of acute eosinophilic inflammation). We also determined the anti-oedematous, analgesic and anti-pyretic effects of L. pacari extract in carrageenan-induced paw oedema, acetic acid writhing and LPS-induced fever, respectively. L. pacari extract significantly inhibited leucocyte recruitment into the peritoneal cavity induced by beta-glucan. In addition, the L. pacari extract presented significant analgesic, anti-oedematous and anti-pyretic effects. Bioassay-guided fractionation of the L. pacari extract in the F1 model led us to identify ellagic acid. As did the extract, ellagic acid presented anti-inflammatory, anti-oedematous and analgesic effects. However, ellagic acid had no anti-pyretic effect, suggesting that other compounds present in the plant stem are responsible for this effect. Nevertheless, our results demonstrate potential therapeutic effects of L. pacari extract and ellagic acid, providing new prospects for the development of drugs to treat pain, oedema and inflammation.

  19. Highlights in pathogenic fungal biofilms.

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    Sardi, Janaina De Cássia Orlandi; Pitangui, Nayla De Souza; Rodríguez-Arellanes, Gabriela; Taylor, Maria Lucia; Fusco-Almeida, Ana Maria; Mendes-Giannini, Maria José Soares

    2014-01-01

    A wide variety of fungi have demonstrated the ability to colonize surfaces and form biofilms. Most studies on fungal biofilms have focused on Candida albicans and more recently, several authors have reported the involvement of other genera of yeasts and Candida species, as well as of filamentous fungi in the formation of biofilms, including: Cryptococcus neoformans, Cryptococcus gattii, Rhodotorula species, Aspergillus fumigatus, Malassezia pachydermatis, Histoplasma capsulatum, Paracoccidioides brasiliensis, Pneumocystis species, Coccidioides immitis, Fusarium species, Saccharomyces cerevisiae, Trichosporon asahii, Mucorales and Blastoschizomyces. There is a current interest in describing the particular characteristics of the biofilm formation by of these fungi. A major concern is the control of biofilms, requiring knowledge of the biofilm mechanisms. However, our knowledge of these microbial communities is limited, due to the complexity of these systems and metabolic interactions that remain unknown. This mini-review aims to highlight recently discovered fungal biofilms and to compare them with the current knowledge on biofilms. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012). Copyright © 2013 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.

  20. Neuroinfection survey at a neurological ward in a Brazilian tertiary teaching hospital

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    Paulo E Marchiori

    2011-01-01

    Full Text Available OBJECTIVES: This study was undertaken to characterize the neuroinfection profile in a tertiary neurological ward. INTRODUCTION: Neuroinfection is a worldwide concern and bacterial meningitis, tetanus and cerebral malaria have been reported as the commonest causes in developing countries. METHODS: From 1999 to 2007, all patients admitted to the Neurology Ward of Hospital das Clínicas, São Paulo University School of Medicine because of neuroinfection had their medical records reviewed. Age, gender, immunological status, neurological syndrome at presentation, infectious agent and clinical outcome were recorded. RESULTS: Three hundred and seventy four cases of neuroinfectious diseases accounted for 4.2% of ward admissions and the identification of infectious agent was successful in 81% of cases. Mean age was 40.5 + 13.4 years, 63.8% were male, 19.7% were immunocompromised patients and meningoencephalitis was the most common clinical presentation despite infectious agent. Viruses and bacteria were equally responsible for 29.4% of neuroinfectious diseases; parasitic, fungal and prion infections accounted for 28%, 9.6% and 3.5% respectively. Human immunodeficiency virus (HIV, herpes simplex virus 1 (HSV1, Mycobacterium tuberculosis, Treponema pallidum, Taenia solium, Schistosoma mansoni, Cryptococcus neoformans and Histoplasma capsulatum were the more common infectious pathogens in the patients. Infection mortality rate was 14.2%, of which 62.3% occurred in immunocompetent patients. CONCLUSION: Our institution appeared to share some results with developed and developing countries. Comparison with literature may be considered as quality control to health assistance.

  1. Morphological changes in the digestive system of 322 necropsies of patients with acquired immune deficiency syndrome: comparison of findings pre- and post-HAART (Highly Active Antiretroviral Therapy

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    Lucinda Calheiros Guimarães

    Full Text Available ABSTRACT Involvement of the digestive system in AIDS pathologies or injuries is frequent. Aiming at comparing the frequency, the importance that these lesions have for death and the survival time in patients using or not using HAART, we studied 322 necropsies classified as follows: Group A - without antiretroviral drugs (185 cases; B - one or two antiretroviral drugs or HAART for less than six months (83 cases; C - HAART for six months or longer (54 cases. In the overall analysis of the digestive system, changes were present in 73.6% of cases. The most frequent was Candida infection (22.7%, followed by cytomegalovirus (19.2%, Histoplasma capsulatum (6.5%, mycobacteria (5.6%, and Toxoplasma gondii (4.3%. T. gondii infection was more frequent in group A compared with group C, and cytomegalovirus (CMV was more frequent in group A compared with groups B and C (p < 0.05; 2.2% of the deaths were due to gastrointestinal bleeding. Regarding the segments, only in the large intestine, and only cytomegalovirus, were more frequent in group A compared with group C. We conclude that digestive system infections are still frequent, even with the use of HAART. However, the average survival time in group C was more than three times greater than the one in group A and nearly double that of group B, demonstrating the clear benefit of this therapy.

  2. Acquired immunodeficiency syndrome and its ocular complications

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    Rao Narsing

    1994-01-01

    Full Text Available Human immunodeficiency virus infection is the first major pandemic of the 20th century. At present, almost 10 million people are known to be infected with this virus, and it is estimated that by the year 2000, approximately 40 million people will be infected. Transmission of this deadly infection is predominantly by sexual contact. Individuals infected with this virus pass through several predictable stages with progressive decrease in circulating CD4+ T cells. During the advanced stage, these patients develop various opportunistic infections or malignancies, or both. It is this advanced stage that was first recognized as AIDS, which has a 100% mortality rate. The opportunistic organisms that can involve the eye in patients with AIDS include cytomegalovirus, herpes zoster, Toxoplasma gondii, Mycobacterium tuberculosis, Cryptococcus neoformans, Mycobacterium avium-intracellulare, Pneumocystis carinii, Histoplasma capsulatum, Candida, and others. Intraocular lesions from these agents often represent disseminated infections. Visual morbidity occurs secondary to retinitis due to cytomegalovirus, herpes zoster, or Toxoplasma gondii. Anti-viral agents such as ganciclovir or foscarnet are effective against cytomegalovirus infection. The role of the ophthalmologist in the diagnosis and management of AIDS is becoming increasingly important. Not only does the eye reflect systemic disease, but ocular involvement may often precede systemic manifestations. In the AIDS patient, the ophthalmologist thus has an opportunity to make not only a slight-saving, but also life-saving diagnosis of disseminated opportunistic infections.

  3. [Disseminated histoplasmosis treated by boluses of fluconazole].

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    Mandengue Ebenye, C; Takuefou Mfangam, B; Nouédoui, C; Atangana, P J A

    2015-01-01

    We report a case in which an HIV-infected man was cured of disseminated histoplasmosis (Histoplasma capsulatum var duboisii) after treatment by high-dose fluconazole (1,600 mg taken four times daily) for 2 months, combined with active antiretroviral therapy. The choice of fluconazole at this dosage was motivated by its availability as a generic and thus inexpensive medication, the patient's precarious status, and his critical clinical condition. At the end of the second month of treatment, the patient chose to stop the fluconazole, which he could no longer afford, while continuing the antiretroviral treatment, which was free. The clinical and laboratory improvement observed from the first week has continued to progress for more than 8 months after fluconazole treatment stopped. This single case needs - and deserves - to be confirmed in a series of patients. Nonetheless it makes it possible to envision fluconazole as a low-cost and efficacious antifungal agent for the treatment of disseminated histoplasmosis in AIDS patients in sub-Saharan Africa.

  4. High Mortality and Coinfection in a Prospective Cohort of Human Immunodeficiency Virus/Acquired Immune Deficiency Syndrome Patients with Histoplasmosis in Guatemala.

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    Samayoa, Blanca; Roy, Monika; Cleveland, Angela Ahlquist; Medina, Narda; Lau-Bonilla, Dalia; Scheel, Christina M; Gomez, Beatriz L; Chiller, Tom; Arathoon, Eduardo

    2017-07-01

    Histoplasmosis is one of the most common and deadly opportunistic infections among persons living with human immunodeficiency virus (HIV)/acquired immune deficiency syndrome in Latin America, but due to limited diagnostic capacity in this region, few data on the burden and clinical characteristics of this disease exist. Between 2005 and 2009, we enrolled patients ≥ 18 years of age with suspected histoplasmosis at a hospital-based HIV clinic in Guatemala City. A case of suspected histoplasmosis was defined as a person presenting with at least three of five clinical or radiologic criteria. A confirmed case of histoplasmosis was defined as a person with a positive culture or urine antigen test for Histoplasma capsulatum . Demographic and clinical data were also collected and analyzed. Of 263 enrolled as suspected cases of histoplasmosis, 101 (38.4%) were confirmed cases. Median time to diagnosis was 15 days after presentation (interquartile range [IQR] = 5-23). Crude overall mortality was 43.6%; median survival time was 19 days (IQR = 4-69). Mycobacterial infection was diagnosed in 70 (26.6%) cases; 26 (25.7%) histoplasmosis cases were coinfected with mycobacteria. High mortality and short survival time after initial symptoms were observed in patients with histoplasmosis. Mycobacterial coinfection diagnoses were frequent, highlighting the importance of pursuing diagnoses for both diseases.

  5. High diversity of polyketide synthase genes and the melanin biosynthesis gene cluster in Penicillium marneffei.

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    Woo, Patrick C Y; Tam, Emily W T; Chong, Ken T K; Cai, James J; Tung, Edward T K; Ngan, Antonio H Y; Lau, Susanna K P; Yuen, Kwok-Yung

    2010-09-01

    Despite the unique phenotypic properties and clinical importance of Penicillium marneffei, the polyketide synthase genes in its genome have never been characterized. Twenty-three putative polyketide synthase genes and two putative polyketide synthase nonribosomal peptide-synthase hybrid genes were identified in the P. marneffei genome, a diversity much higher than found in other pathogenic thermal dimorphic fungi, such as Histoplasma capsulatum (one polyketide synthase gene) and Coccidioides immitis (10 polyketide synthase genes). These genes were evenly distributed on the phylogenetic tree with polyketide synthase genes of Aspergillus and other fungi, indicating that the high diversity was not a result of lineage-specific gene expansion through recent gene duplication. The melanin-biosynthesis gene cluster had gene order and orientations identical to those in the Talaromyces stipitatus (a teleomorph of Penicillium emmonsii) genome. Phylogenetically, all six genes of the melanin-biosynthesis gene cluster in P. marneffei were also most closely related to those in T. stipitatus, with high bootstrap supports. The polyketide synthase gene of the melanin-biosynthesis gene cluster (alb1) in P. marneffei was knocked down, which was accompanied by loss of melanin pigment production and reduced ornamentation in conidia. The survival of mice challenged with the alb1 knockdown mutant was significantly better than those challenged with wild-type P. marneffei (P melanin-biosynthesis gene cluster contributed to virulence through decreased susceptibility to killing by hydrogen peroxide. © 2010 The Authors Journal compilation © 2010 FEBS.

  6. Frecuencia de micosis en pacientes inmunosuprimidos de un hospital regional de la Ciudad de México

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    Hernández-Hernández Francisca

    2003-01-01

    Full Text Available OBJETIVO: Aislar e identificar hongos en diferentes especímenes de pacientes inmunocomprometidos, atendidos en un hospital de la Ciudad de México, y determinar su asociación con micosis. MATERIAL Y MÉTODOS: Se realizó un estudio de tipo observacional transversal en pacientes del Hospital Regional General Ignacio Zaragoza, de junio de 1999 a mayo de 2000. De 108 pacientes se procesaron 268 especímenes para estudio micológico que incluyó examen directo, frotis, cultivos y microcultivos en medios específicos además de pruebas bioquímicas. La mayoría de pacientes tenía diagnóstico clínico de tuberculosis pulmonar y de síndrome de inmunodeficiencia adquirida. RESULTADOS: Se obtuvieron 183 aislamientos de levaduras y 66 de hongos filamentosos. Se diagnosticaron 45 micosis que en su mayoría correspondieron a candidosis pulmonar (32 casos. Las especies de Candida más frecuentes asociadas a patología fueron Candida albicans y C parapsilosis. Se obtuvieron cinco aislamientos de Cryptococcus neoformans variedad neoformans, uno de C albidus, tres de Histoplasma capsulatum y uno de Geotrichum candidum, asociados a infección micótica. CONCLUSIONES: El 41.6% de los pacientes estudiados presentaron una micosis asociada principalmente a síndrome de inmunodeficiencia adquirida y a tuberculosis pulmonar.

  7. Mycobacterial and nonbacterial pulmonary complications in hospitalized patients with human immunodeficiency virus infection: A prospective, cohort study

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    Afessa Bekele

    2001-09-01

    Full Text Available Abstract Background A prospective observational study was done to describe nonbacterial pulmonary complications in hospitalized patients with human immunodeficiency virus (HIV infection. Methods The study included 1,225 consecutive hospital admissions of 599 HIV-infected patients treated from April 1995 through March 1998. Data included demographics, risk factors for HIV infection, Acute Physiology and Chronic Health Evaluation (APACHE II score, pulmonary complications, CD4+ lymphocyte count, hospital stay and case-fatality rate. Results Patient age (mean ± SD was 38.2 ± 8.9 years, 62% were men, and 84% were African American. The median APACHE II score was 14, and median CD4+ lymphocyte count was 60/μL. Pulmonary complications were Pneumocystis carinii pneumonia (85 in 78 patients, Mycobacterium avium complex (51 in 38, Mycobacterium tuberculosis (40 in 35, Mycobacterium gordonae (11 in 11, Mycobacterium kansasii (10 in 9, Cytomegalovirus (10 in 10, Nocardia asteroides (3 in 3, fungus ball (2 in 2, respiratory syncytial virus (1, herpes simplex virus (1, Histoplasma capsulatum (1, lymphoma (3 in 3, bronchogenic carcinoma (2 in 2, and Kaposi sarcoma (1. The case-fatality rate of patients was 11% with Pneumocystis carinii pneumonia; 5%, Mycobacterium tuberculosis; 6%, Mycobacterium avium complex; and 7%, noninfectious pulmonary complications. Conclusion Most pulmonary complications in hospitalized patients with HIV are from Pneumocystis and mycobacterial infection.

  8. Systemic fungal infections in patients with human inmunodeficiency virus.

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    Rodríguez-Cerdeira, C; Arenas, R; Moreno-Coutiño, G; Vásquez, E; Fernández, R; Chang, P

    2014-01-01

    Histoplasmosis is a systemic infection caused by the dimorphic fungus Histoplasma capsulatum. In immunocompromised patients, primary pulmonary infection can spread to the skin and meninges. Clinical manifestations appear in patients with a CD4(+) lymphocyte count of less than 150 cells/μL. Coccidioidomycosis is a systemic mycosis caused by Coccidioides immitis and Coccidioides posadasii. It can present as diffuse pulmonary disease or as a disseminated form primarily affecting the central nervous system, the bones, and the skin. Cryptococcosis is caused by Cryptococcus neoformans (var. neoformans and var. grubii) and Cryptococcus gattii, which are members of the Cryptococcus species complex and have 5 serotypes: A, B, C, D, and AD. It is a common opportunistic infection in patients with human immunodeficiency virus (HIV)/AIDS, even those receiving antiretroviral therapy. Histopathologic examination and culture of samples from any suspicious lesions are essential for the correct diagnosis of systemic fungal infections in patients with HIV/AIDS. Copyright © 2011 Elsevier España, S.L. and AEDV. All rights reserved.

  9. Autopsy report of disseminated histoplasmosis: An important differential of adrenal enlargement

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    Alka Dattaray Kalgutkar

    2015-01-01

    Full Text Available A 57-year-old diabetic male, presented with altered sensorium since 2 days. He had a history of weight loss, loss of appetite, intermittent low-grade fever of 6 months duration. He had been administered empirical antituberculous therapy some months ago with no alleviation of symptoms. On examination, he was asthenic, normotensive, and anemic with hepatosplenomegaly. Ultra-sonography abdomen revealed bilateral enlarged adrenals with hepatosplenomegaly. Clinically impression was of tuberculosis disseminated malignancy. He expired within 2 days of admission. At autopsy, both the adrenals were markedly enlarged showing extensive areas of necrosis on the cut surface. Histology showed them to be brimming with colonies of yeast-forms of Histoplasma capsulatum. These organisms were also seen in the spleen, lungs and kidneys. The patient had died of septicemia following disseminated histoplasmosis that was, unfortunately, not diagnosed during his life. Histoplasmosis is amenable to treatment with Amphotericin B. Prompt diagnosis and treatment could have helped in salvaging this patient.

  10. Glutathione-dependent extracellular ferric reductase activities in dimorphic zoopathogenic fungi

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    Zarnowski, Robert; Woods, Jon P.

    2009-01-01

    In this study, extracellular glutathione-dependent ferric reductase (GSH-FeR) activities in different dimorphic zoopathogenic fungal species were characterized. Supernatants from Blastomyces dermatitidis, Histoplasma capsulatum, Paracoccidioides brasiliensis and Sporothrix schenckii strains grown in their yeast form were able to reduce iron enzymically with glutathione as a cofactor. Some variations in the level of reduction were noted amongst the strains. This activity was stable in acidic, neutral and slightly alkaline environments and was inhibited when trivalent aluminium and gallium ions were present. Using zymography, single bands of GSH-FeRs with apparent molecular masses varying from 430 to 460 kDa were identified in all strains. The same molecular mass range was determined by size exclusion chromatography. These data demonstrate that dimorphic zoopathogenic fungi produce and secrete a family of similar GSH-FeRs that may be involved in the acquisition and utilization of iron. Siderophore production by these and other fungi has sometimes been considered to provide a full explanation of iron acquisition in these organisms. Our work reveals an additional common mechanism that may be biologically and pathogenically important. Furthermore, while some characteristics of these enzymes such as extracellular location, cofactor utilization and large size are not individually unique, when considered together and shared across a range of fungi, they represent an important novel physiological feature. PMID:16000713

  11. Polyketides, Toxins and Pigments in Penicillium marneffei

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    Emily W. T. Tam

    2015-10-01

    Full Text Available Penicillium marneffei (synonym: Talaromyces marneffei is the most important pathogenic thermally dimorphic fungus in China and Southeastern Asia. The HIV/AIDS pandemic, particularly in China and other Southeast Asian countries, has led to the emergence of P. marneffei infection as an important AIDS-defining condition. Recently, we published the genome sequence of P. marneffei. In the P. marneffei genome, 23 polyketide synthase genes and two polyketide synthase-non-ribosomal peptide synthase hybrid genes were identified. This number is much higher than those of Coccidioides immitis and Histoplasma capsulatum, important pathogenic thermally dimorphic fungi in the Western world. Phylogenetically, these polyketide synthase genes were distributed evenly with their counterparts found in Aspergillus species and other fungi, suggesting that polyketide synthases in P. marneffei did not diverge from lineage-specific gene duplication through a recent expansion. Gene knockdown experiments and ultra-high performance liquid chromatography-photodiode array detector/electrospray ionization-quadruple time of flight-mass spectrometry analysis confirmed that at least four of the polyketide synthase genes were involved in the biosynthesis of various pigments in P. marneffei, including melanin, mitorubrinic acid, mitorubrinol, monascorubrin, rubropunctatin, citrinin and ankaflavin, some of which were mycotoxins and virulence factors of the fungus.

  12. Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives.

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    Sharpton, Thomas J; Stajich, Jason E; Rounsley, Steven D; Gardner, Malcolm J; Wortman, Jennifer R; Jordar, Vinita S; Maiti, Rama; Kodira, Chinnappa D; Neafsey, Daniel E; Zeng, Qiandong; Hung, Chiung-Yu; McMahan, Cody; Muszewska, Anna; Grynberg, Marcin; Mandel, M Alejandra; Kellner, Ellen M; Barker, Bridget M; Galgiani, John N; Orbach, Marc J; Kirkland, Theo N; Cole, Garry T; Henn, Matthew R; Birren, Bruce W; Taylor, John W

    2009-10-01

    While most Ascomycetes tend to associate principally with plants, the dimorphic fungi Coccidioides immitis and Coccidioides posadasii are primary pathogens of immunocompetent mammals, including humans. Infection results from environmental exposure to Coccidiodies, which is believed to grow as a soil saprophyte in arid deserts. To investigate hypotheses about the life history and evolution of Coccidioides, the genomes of several Onygenales, including C. immitis and C. posadasii; a close, nonpathogenic relative, Uncinocarpus reesii; and a more diverged pathogenic fungus, Histoplasma capsulatum, were sequenced and compared with those of 13 more distantly related Ascomycetes. This analysis identified increases and decreases in gene family size associated with a host/substrate shift from plants to animals in the Onygenales. In addition, comparison among Onygenales genomes revealed evolutionary changes in Coccidioides that may underlie its infectious phenotype, the identification of which may facilitate improved treatment and prevention of coccidioidomycosis. Overall, the results suggest that Coccidioides species are not soil saprophytes, but that they have evolved to remain associated with their dead animal hosts in soil, and that Coccidioides metabolism genes, membrane-related proteins, and putatively antigenic compounds have evolved in response to interaction with an animal host.

  13. Histoplasmosis del sistema nervioso central en un paciente inmunocompetente

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    Natalia Osorio

    2014-12-01

    Full Text Available La histoplasmosis es una afección polifacética producida por el hongo dimorfo Histoplasma capsulatum, cuyas esporas son inhaladas y llegan al pulmón, órgano primario de infección. La forma meníngea, considerada como una de las manifestaciones más graves de esta micosis, suele presentarse en individuos con alteraciones en la inmunidad celular: pacientes con síndrome de inmunodeficiencia humana adquirida, con lupus eritematoso sistémico o con trasplante de órgano sólido, así como en lactantes, debido a su inmadurez inmunológica. La forma de presentación más usual es de resolución espontánea y se observa en individuos inmunocompetentes que se han expuesto a altas concentraciones de conidias y fragmentos miceliares del hongo. En estas personas, la afección se manifiesta por trastornos pulmonares y por la posterior diseminación a otros órganos y sistemas. Se presenta un caso de histoplasmosis del sistema nervioso central en un niño inmunocompetente.

  14. Bacteremia and fungemia in patients with the acquired immunodeficiency syndrome.

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    Whimbey, E; Gold, J W; Polsky, B; Dryjanski, J; Hawkins, C; Blevins, A; Brannon, P; Kiehn, T E; Brown, A E; Armstrong, D

    1986-04-01

    Forty-nine episodes of bacteremia and fungemia occurred in 38 of 336 patients with the acquired immunodeficiency syndrome seen at our institution since 1980. There were five types of infections. Infections commonly associated with a T-cell immunodeficiency disorder comprised 16 episodes and included those with Salmonella species, Listeria monocytogenes, Cryptococcus neoformans, and Histoplasma capsulatum. Infections commonly associated with a B-cell immunodeficiency disorder included those with Streptococcus pneumoniae and Haemophilus influenzae. Infections occurring with neutropenia were caused by Pseudomonas aeruginosa, Staphylococcus epidermidis, and Streptococcus faecalis. Other infections occurring in the hospital were caused by Candida albicans, Staphylococcus epidermidis, enteric gram-negative rods, Staphylococcus aureus, and mixed S. aureus and group G streptococcus. Other infections occurring out of the hospital included those with S. aureus, Clostridium perfringens, Shigella sonnei, Pseudomonas aeruginosa, and group B streptococcus. Because two thirds of the septicemias were caused by organisms other than T-cell opportunists, these pathogens should be anticipated during diagnostic evaluation and when formulating empiric therapy.

  15. Morphological changes in the digestive system of 322 necropsies of patients with acquired immune deficiency syndrome: comparison of findings pre- and post-HAART (Highly Active Antiretroviral Therapy).

    Science.gov (United States)

    Guimarães, Lucinda Calheiros; Silva, Ana Cristina Araújo Lemos da; Micheletti, Adilha Misson Rua; Moura, Everton Nunes Melo; Silva-Vergara, Mario Léon; Tostes, Sebastião; Adad, Sheila Jorge

    2017-04-03

    Involvement of the digestive system in AIDS pathologies or injuries is frequent. Aiming at comparing the frequency, the importance that these lesions have for death and the survival time in patients using or not using HAART, we studied 322 necropsies classified as follows: Group A - without antiretroviral drugs (185 cases); B - one or two antiretroviral drugs or HAART for less than six months (83 cases); C - HAART for six months or longer (54 cases). In the overall analysis of the digestive system, changes were present in 73.6% of cases. The most frequent was Candida infection (22.7%), followed by cytomegalovirus (19.2%), Histoplasma capsulatum (6.5%), mycobacteria (5.6%), and Toxoplasma gondii (4.3%). T. gondii infection was more frequent in group A compared with group C, and cytomegalovirus (CMV) was more frequent in group A compared with groups B and C (p digestive system infections are still frequent, even with the use of HAART. However, the average survival time in group C was more than three times greater than the one in group A and nearly double that of group B, demonstrating the clear benefit of this therapy.

  16. Histoplasmosis suprarrenal incidental

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    C. Nine

    2002-12-01

    Full Text Available El hallazgo de masas adrenales detectadas mediante técnicas ecográficas o tomográficas solicitadas por algún otro motivo, y por ello incidentales, constituye un nuevo desafío diagnóstico. El paciente que presentamos se encontraba asintomático y no refería antecedentes patológicos. Una ecografía mostró agrandamiento de la glándula suprarrenal derecha, que medía 66 por 33mm. La tomografía computada mostró agrandamiento irregular de ambas suprarrenales. Se descartaron neoplasias extra-adrenales y tumores hiperfuncionantes. Se realizó una biopsia guiada por tomografía, y el estudio histopatológico informó Histoplasma capsulatum. El paciente vivía en una zona endémica para esta enfermedad y, por exposición laboral, tenía contacto diario con excremento de aves. La histoplasmosis es una causa infrecuente de masas adrenales bilaterales en el huesped inmu-nocompetente.

  17. Organization and Evolutionary Trajectory of the Mating Type (MAT) Locus in Dermatophyte and Dimorphic Fungal Pathogens▿ †

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    Li, Wenjun; Metin, Banu; White, Theodore C.; Heitman, Joseph

    2010-01-01

    Sexual reproduction in fungi is governed by a specialized genomic region, the mating type (MAT) locus, whose gene identity, organization, and complexity are diverse. We identified the MAT locus of five dermatophyte fungal pathogens (Microsporum gypseum, Microsporum canis, Trichophyton equinum, Trichophyton rubrum, and Trichophyton tonsurans) and a dimorphic fungus, Paracoccidioides brasiliensis, and performed phylogenetic analyses. The identified MAT locus idiomorphs of M. gypseum control cell type identity in mating assays, and recombinant progeny were produced. Virulence tests in Galleria mellonella larvae suggest the two mating types of M. gypseum may have equivalent virulence. Synteny analysis revealed common features of the MAT locus shared among these five dermatophytes: namely, a small size (∼3 kb) and a novel gene arrangement. The SLA2, COX13, and APN2 genes, which flank the MAT locus in other Ascomycota are instead linked on one side of the dermatophyte MAT locus. In addition, the transcriptional orientations of the APN2 and COX13 genes are reversed compared to the dimorphic fungi Histoplasma capsulatum, Coccidioides immitis, and Coccidioides posadasii. A putative transposable element, pogo, was found to have inserted in the MAT1-2 idiomorph of one P. brasiliensis strain but not others. In conclusion, the evolution of the MAT locus of the dermatophytes and dimorphic fungi from the last common ancestor has been punctuated by both gene acquisition and expansion, and asymmetric gene loss. These studies further support a foundation to develop molecular and genetic tools for dermatophyte and dimorphic human fungal pathogens. PMID:19880755

  18. Organization and evolutionary trajectory of the mating type (MAT) locus in dermatophyte and dimorphic fungal pathogens.

    Science.gov (United States)

    Li, Wenjun; Metin, Banu; White, Theodore C; Heitman, Joseph

    2010-01-01

    Sexual reproduction in fungi is governed by a specialized genomic region, the mating type (MAT) locus, whose gene identity, organization, and complexity are diverse. We identified the MAT locus of five dermatophyte fungal pathogens (Microsporum gypseum, Microsporum canis, Trichophyton equinum, Trichophyton rubrum, and Trichophyton tonsurans) and a dimorphic fungus, Paracoccidioides brasiliensis, and performed phylogenetic analyses. The identified MAT locus idiomorphs of M. gypseum control cell type identity in mating assays, and recombinant progeny were produced. Virulence tests in Galleria mellonella larvae suggest the two mating types of M. gypseum may have equivalent virulence. Synteny analysis revealed common features of the MAT locus shared among these five dermatophytes: namely, a small size ( approximately 3 kb) and a novel gene arrangement. The SLA2, COX13, and APN2 genes, which flank the MAT locus in other Ascomycota are instead linked on one side of the dermatophyte MAT locus. In addition, the transcriptional orientations of the APN2 and COX13 genes are reversed compared to the dimorphic fungi Histoplasma capsulatum, Coccidioides immitis, and Coccidioides posadasii. A putative transposable element, pogo, was found to have inserted in the MAT1-2 idiomorph of one P. brasiliensis strain but not others. In conclusion, the evolution of the MAT locus of the dermatophytes and dimorphic fungi from the last common ancestor has been punctuated by both gene acquisition and expansion, and asymmetric gene loss. These studies further support a foundation to develop molecular and genetic tools for dermatophyte and dimorphic human fungal pathogens.

  19. Co-infection of disseminated histoplasmosis and tuberculosis in an AIDS patient

    Directory of Open Access Journals (Sweden)

    Bruno Tomazini

    2013-10-01

    Full Text Available Histoplasmosis is a fungal disease caused by the dimorphic fungus Histoplasma capsulatum, recognized as an AIDS-defining illness since the Center for Disease Control’s revision criteria in 1985. This infection is reported to be present in 5-20% of AIDS patients, and in 95% of the cases it is manifested in its disseminated form. Serum antibodies and/or antigen research can make diagnosis, but the demonstration of the agent by culture or histopathological examination remains the gold standard methods. Co-infections in patients with AIDS are well known; however, reports on disseminated tuberculosis and histoplasmosis are scarce. The authors report the case of a female patient who presented a short-course history of weight loss, fever, and mild respiratory symptoms, with hepatosplenomegaly and lymphadenopathy. Laboratory workup called attention to anemia, altered liver, canalicular enzymes, liver function tests, high titer of lactate dehydrogenase (LDH, and pulmonary nodules on thoracic computed tomography. Incidental finding of yeast forms within the leukocytes during a routine blood cell count highlighted the diagnosis of histoplasmosis. The patient started receiving amphotericin B but succumbed soon after. The authors emphasize the possibility of this co-infection, the diagnosis of severe infection through the finding of yeast forms within peripheral leukocytes, and for the high titer of LDH in aiding the differential diagnosis.

  20. [Disseminated histoplasmosis in children].

    Science.gov (United States)

    Ramón-García, G; Peña-Alonso, Y R; Ontiveros-Nevares, P G

    1993-12-01

    Disseminated histoplasmosis is a rare and severe form of the infection due to the fungus Histoplasma capsulatum. This form of disease is seen mainly in patients with some sort of immune deficiency either primary or secondary, in children under two years and in elderly people. An autopsy review at the Hospital Infantil de México Federico Gómez disclosed eight cases of lethal disseminated histoplasmosis in an eight year period. All patients were malnourished, one of them also had a primary immunodeficiency an another had acquired immune deficiency syndrome. The age range was from two months to seven years; five patients were males. The organs more frequently affected were lungs, liver and spleen. In seven patients the diagnosis was established at postmortem study. The patients came from Morelos, Veracruz, Guerrero and Chiapas, regions considered endemic for histoplasmosis. A careful analysis of the clinical symptoms together with a detailed information of the living environment of patients, will lead to an accurate diagnosis and management of children with this disease.

  1. Diagnóstico molecular de histoplasmosis humana en muestras de sangre entera Molecular diagnosis of human histoplasmosis in whole blood samples

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    A. I. Toranzo

    2009-03-01

    Full Text Available Se evaluó el uso de sangre entera para el diagnóstico molecular de histoplasmosis utilizando un método artesanal de extracción de ADN fúngico y una PCR anidada que amplifica una porción del gen HcP100 específica de Histoplasma capsulatum. La sangre entera se trató con liticasa, enzima lisante de Trichoderma harzianum y proteinasa K, seguido de una extracción fenólica. Este tratamiento permitió una lisis completa de las células, mostró buen rendimiento en la obtención de ADN y posibilitó la detección de la banda de 210 pb específica de H. capsulatum en la PCR anidada. El límite de detección fue de 0,25-1 levaduras/ml de sangre. El método se evaluó en 31 muestras de sangre de 19 pacientes con diagnóstico microbiológico de histoplasmosis, en 21 muestras de pacientes con otras micosis o infecciones por micobacterias y en 30 controles sanos. La PCR fue positiva en sangre para 17/19 pacientes con histoplasmosis (14/15 inmunocomprometidos y 3/4 sin inmunocompromiso aparente. Las muestras de sangre de los 30 controles sanos y de 20 pacientes con otras patologías fueron negativas, sólo hubo un falso positivo correspondiente a un paciente con infección por Mycobacterium avium-intracellulare. El método presentó 89% de sensibilidad y 96% de especificidad para el diagnóstico de histoplasmosis en sangre entera.To assess the value of using whole blood samples for the molecular diagnosis of histoplasmosis, we applied an in-house DNA extraction method and a nested PCR targeting a 210 bp specific segment of the Histoplasma capsulatum HcP100 gene. A whole blood volume of 2.5-3 milliliters was centrifuged and the cellular pellet was treated with Trichoderma harzianum lyticase and proteinase K prior to applying a conventional phenol DNA extraction. This procedure allowed complete cell lysis, high DNA yield and specific amplification. The PCR detection limit was 0.25-1 yeast cells/ml of blood sample. The method was assessed on 31 blood

  2. [Efficacy of the treatment and secondary antifungal prophylaxis in AIDS-related histoplasmosis. Experience at the Francisco J. Muñiz Infectious Diseases Hospital in Buenos Aires].

    Science.gov (United States)

    Negroni, Ricardo; Messina, Fernando; Arechavala, Alicia; Santiso, Gabriela; Bianchi, Mario

    Classic histoplasmosis is a systemic endemic mycosis due to Histoplasma capsulatum var. capsulatum. A significant reduction in the morbidity and mortality of AIDS-related histoplasmosis has been observed since the introduction of highly active antiretroviral therapy (HAART) and secondary antifungal prophylaxis. The aim of this study was to determine the current state of prognosis and treatment response of HIV-positive patients with histoplasmosis in the Francisco J. Muñiz Infectious Diseases Hospital in Buenos Aires City. A retrospective study was conducted using the demographic, clinical, immunological and treatment data of 80 patients suffering from AIDS-related histoplasmosis. Of the 80 cases studied 65 were male, the median age was 36 years, with 73.7% of the patients being drug addicts, 82.5% of the patients was not receiving HAART at diagnosis, and 58.7% of the cases had less than 50 CD4+ cells/μl at the beginning of the treatment. The initial phase of treatment consisted of intravenous amphotericin B and/or oral itraconazole for 3 months, with 78.7% of the cases showing a good clinical response. Only 26/63 patients who were discharged from hospital continued with the follow-up of the HAART, secondary prophylaxis with itraconazole or amphotericin B. Secondary prophylaxis was stopped after more than one year of HAART if the patients were asymptomatic, had two CD4+ cell counts greater than 150cells/μl, and undetectable viral loads. No relapses were observed during a two-year follow up after prophylaxis was stopped. The treatment of histoplasmosis in HIV-positive patients was effective in 78.8% of the cases. The combination of HAART and secondary antifungal prophylaxis is safe, well tolerated, and effective. The low adherence of patients to HAART and the lack of laboratory kits for rapid histoplasmosis diagnosis should be addressed in the future. The usefulness of primary antifungal prophylaxis for cryptococcosis and histoplasmosis HIV-positive patients

  3. FungalRV: adhesin prediction and immunoinformatics portal for human fungal pathogens

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    Ramachandran Srinivasan

    2011-04-01

    Full Text Available Abstract Background The availability of sequence data of human pathogenic fungi generates opportunities to develop Bioinformatics tools and resources for vaccine development towards benefitting at-risk patients. Description We have developed a fungal adhesin predictor and an immunoinformatics database with predicted adhesins. Based on literature search and domain analysis, we prepared a positive dataset comprising adhesin protein sequences from human fungal pathogens Candida albicans, Candida glabrata, Aspergillus fumigatus, Coccidioides immitis, Coccidioides posadasii, Histoplasma capsulatum, Blastomyces dermatitidis, Pneumocystis carinii, Pneumocystis jirovecii and Paracoccidioides brasiliensis. The negative dataset consisted of proteins with high probability to function intracellularly. We have used 3945 compositional properties including frequencies of mono, doublet, triplet, and multiplets of amino acids and hydrophobic properties as input features of protein sequences to Support Vector Machine. Best classifiers were identified through an exhaustive search of 588 parameters and meeting the criteria of best Mathews Correlation Coefficient and lowest coefficient of variation among the 3 fold cross validation datasets. The "FungalRV adhesin predictor" was built on three models whose average Mathews Correlation Coefficient was in the range 0.89-0.90 and its coefficient of variation across three fold cross validation datasets in the range 1.2% - 2.74% at threshold score of 0. We obtained an overall MCC value of 0.8702 considering all 8 pathogens, namely, C. albicans, C. glabrata, A. fumigatus, B. dermatitidis, C. immitis, C. posadasii, H. capsulatum and P. brasiliensis thus showing high sensitivity and specificity at a threshold of 0.511. In case of P. brasiliensis the algorithm achieved a sensitivity of 66.67%. A total of 307 fungal adhesins and adhesin like proteins were predicted from the entire proteomes of eight human pathogenic fungal

  4. Histoplasmosis diseminada, linfopenia, y síndrome de Sjögren Disseminated histoplasmosis, lymphopenia and Sjögren´s syndrome

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    Horacio F. Rodrigo

    2012-10-01

    Full Text Available El síndrome de Sjögren es una enfermedad autoinmune caracterizada por disminución de las secreciones de las glándulas exocrinas; puede presentar también diversas alteraciones hemáticas, entre ellas linfopenia. Presentamos el caso de un varón de 28 años que consultó por cefalea de un mes de evolución a la que se agregaron en las últimas 48 horas vómitos y fiebre. Presentaba lesiones en piel de tronco y cara; no tenía rigidez de nuca. Se demostró infección por Histoplasma capsulatum var. capsulatum en piel y líquido cefalorraquídeo, linfopenia, anticuerpos anti Ro-SSA positivos, baja concentración del trazador en centellograma de glándulas salivales e infiltración linfocitaria en glándulas salivales, lo que permitió confirmar al diagnóstico de síndrome de Sjögren. El tratamiento con anfotericina liposomal e itraconazol mejoró el cuadro clínico. Comunicamos este caso para referir que una infección oportunista, como la histoplasmosis diseminada, puede ser una forma poco común de presentación del síndrome de Sjögren.Sjögren's syndrome is an autoimmune disease characterized by decreased exocrine gland secretions; patients may also present several hematological abnormalities, like lymphopenia. We describe the case of a 28 year old man who complained of headache a month of duration, with fever and vomiting in the last 48 hours. He also presented skin lesions on trunk and face, without neck stiffness. The diagnosis of duration infection was confirmed by culture from the skin biopsy and spinal fluid specimens; in addition, the presence of lymphopenia, positive anti Ro-SSA antibodies, poor concentration of the tracer in scintigraphy and lymphocytic infiltration in salivary glands confirmed the diagnosis of Sjögren's syndrome. The patient was successfully treated with liposomal amphotericin and itraconazole. We report this case to emphasize that opportunistic infections, such as disseminated histoplasmosis, may be an

  5. Les Representations Sociales de la Ville Chez les Ecoliers de Deux Villes Europeennes, d'Arles et de Sparte: Une Approche Comparative.

    Science.gov (United States)

    Zafeirakou, Aegli

    1998-01-01

    In an exchange program, 11- and 12-year olds from Arles (France) (n=25) and Sparta (Greece) (n=27) visited each other's cities and explored the culture and history. This study explores the ways they conceptualized the cultural patrimony of both cities through student drawings. The exchange created opportunities for dialog about European culture.…

  6. Three-dimensional mapping of salt load in the Murray-Darling Basin, 1 Steps in calibration of airborne electromagnetic surveys

    NARCIS (Netherlands)

    Cresswell, R.G.; Dent, D.L.; Jones, G.; Galloway, D.

    2004-01-01

    An airborne electromagnetic survey yields a three-dimensional map of ground electrical conductivity. The remotely sensed data are translated into salt load by field and laboratory calibration: drilling, measurement of borehole conductivity, electrical conductivity of 1 : 5 soil¿water extracts

  7. Determining the Statistical Power of the Kolmogorov-Smirnov and Anderson-Darling Goodness-of-Fit Tests via Monte Carlo Simulation

    Science.gov (United States)

    2016-12-01

    KSPower numsamp variance w ratio for ssize=4:2:150 for k=1:10000 x1=normrnd(s,variance,ssize,1); x1= sort (x1); x2=normrnd(5,variance...ssize,1); x2= sort (x2); xtot= sort ([x1;x2]); xtotu=unique( sort ([x1;x2])); l=length(unique( sort ([x1;x2]))); n=length(x1); m=length(x2); tot=n+m...IMPORTANT NOTE: INSERT SAMPLE DATA IN COLUMNS STARTING AT A3 AND B3

  8. Kapteni roll tõendite kogumisel / North East Branch of the Nautical Institute ; [tlk. Uno Laur ; toim. Viiu Suurväli ; eess.: Gerald Darling

    Index Scriptorium Estoniae

    1997-01-01

    Kapteni osa, logiraamatud ; lasti kahjustus, kaotus ja puudujääk ; kindlustus ja laevarike ; vaegtäitmise- ja ülekulupretensioonid ; ohtlikud sadamad ja laadimispaigad ; statsionaarsete ja ujuvobjektide vigastused ; õlireostus ; üldavarii ; päästmine ; laevakokkupõrked ; töötülide lahendamine ja distsiplinaarküsimused ; õnnetusjuhtumid inimestega ; piletita reisijad , põgenikud

  9. Kapteni roll tõendite kogumisel / North East Branch of the Nautical Institute ; [tlk. Uno Laur ; toim. Viiu Suurväli ; eess.: Gerald Darling

    Index Scriptorium Estoniae

    1998-01-01

    Kapteni osa, logiraamatud ; lasti kahjustus, kaotus ja puudujääk ; kindlustus ja laevarike ; vaegtäitmise- ja ülekulupretensioonid ; ohtlikud sadamad ja laadimispaigad ; statsionaarsete ja ujuvobjektide vigastused ; õlireostus ; üldavarii ; päästmine ; laevakokkupõrked ; töötülide lahendamine ja distsiplinaarküsimused ; õnnetusjuhtumid inimestega ; piletita reisijad , põgenikud

  10. PREFACE: Proceedings of the 7th Edoardo Amaldi Conference on Gravitational Waves (Amaldi7), Sydney Convention and Exhibition Centre, Darling Harbour, Sydney, Australia, 8 14 July 2007

    Science.gov (United States)

    Scott, Susan M.; McClelland, David E.

    2008-07-01

    At GR17 in Dublin in 2004, it was decided to hold GR18 in Sydney in 2007. Every six years, the GR conference (held every three years) and Amaldi meeting (held every two years) occur in the same year around July. This was to be the case in 2007. By mutual agreement of the International Society on General Relativity and Gravitation (ISGRG), which oversees the GR conferences and The Gravitational Wave International Committee (GWIC), which oversees the Amaldi meetings, it was decided to hold these two important conferences concurrently, for the first time, at the same venue, namely Sydney. At a time when the gravitational wave community was beginning to explore the possibility of searches to probe various aspects of the theory, the vision was to bring that community together with the community of gravitational theorists in order to better appreciate the work being done by both parties and to explore possibilities for future research using the mutual expertise. The logistics of running two such large meetings concurrently were considerable. The format agreed upon by the ISGRG and GWIC was the following: common plenary sessions in the mornings from Monday to Friday; six parallel GR workshop sessions and an Amaldi session each afternoon from Monday to Friday (except Wednesday); a combined poster session on Wednesday; a full day of Amaldi sessions on the final day (Saturday). The scientific programme for GR18 was overseen by a Scientific Organising Committee established by the ISGRG and chaired by Professor Sathyaprakash. The scientific programme for Amaldi7 was overseen by GWIC chaired by Professor Cerdonio. One of the highlights of the conferences was the breadth and quality of the plenary programme put together by the scientific committees. Not only did these talks give an excellent snapshot of the entire field at this time, but they also explored the interfaces with other related fields, which proved of special interest to participants. We were given superb overviews of the state of the art of: observational handles on dark energy; collider physics experiments designed to probe cosmology; gravitational dynamics of large stellar systems; and the use of analogue condensed-matter systems in the laboratory to investigate black hole event horizons. In the more mainstream areas we were given timely reviews of: the Gravity Probe B and STEP missions; quasi-local black hole horizons and their applications; cosmic censorship; the spin foam model approach to quantum gravity; the causal dynamical triangulations approach to quantum gravity; superstring theory applied to questions in particle physics; the current status and prospects for gravitational wave astronomy; ground-based gravitational wave detection; and technology developments for the future LISA mission. A special issue of Classical and Quantum Gravity (Volume 25, Number 11, 7 June 2008) is published as the proceedings of GR18 and Amaldi7. It contains the overview articles by the plenary speakers, the summaries of each GR18 workshop parallel session as provided by the workshop chairs, and the highlights of the Amaldi7 meeting as selected by the Amaldi7 chairs. Other Amaldi7 talks and posters appear in this refereed issue of the electronic Journal of Physics: Conference Series. This issue of JPCS and the CQG Special Issue are electronically linked. The conference organisers would like to acknowledge the financial support of: The Australian National University; IUPAP; The Australian Institute of Physics; BHP Billiton; The University of Western Australia; The University of New South Wales; The Institute of Physics; The Gravity Research Foundation; SGI; CosNet; The Australian Mathematical Sciences Institute; Springer; Duraduct; the New South Wales Government; The Australasian Society for General Relativity and Gravitation; the Mexican GR bid; the Centre for Precision Optics; The Anglo-Australian Observatory; Newspec; CSIRO; and The University of Melbourne. We would like to thank the GR18 Scientific Organising Committee, GWIC and the Local Organising Committee for all their hard work in putting together these very successful combined conferences, which attracted 520 participants. Many of the practical aspects of the organisation were handled by the event management company Conexion, and their professionalism, expertise and dedication were greatly appreciated. Finally, we would like to thank all the participants for their lively and colourful contributions to making these conferences a success. Susan M Scott Chair, Local Organising Committee David E McClelland Deputy Chair, Local Organising Committee Centre for Gravitational Physics, The Australian National University, Australia Guest Editors Participants gather prior to opening ceremony Participants gather prior to opening ceremony Participants entering auditorium for opening ceremony Participants entering auditorium for opening ceremony Chair of Local Organising Committee Susan M Scott opening ceremony Chair of Local Organising Committee Susan M Scott opening ceremony President of the International Society on General Relativity and Gravitation Clifford M Will opening ceremony President of the International Society on General Relativity and Gravitation Clifford M Will opening ceremony Amusing moment at opening ceremony Amusing moment at opening ceremony Chair of the Gravitational Wave International Committee James Hough opening ceremony Chair of the Gravitational Wave International Committee James Hough opening ceremony Welcome to the land by traditional land owner Welcome to the land by traditional land owner Welcome to the land by traditional land owner Welcome to the land by traditional land owner First plenary speaker Stan E Whitcomb First plenary speaker Stan E Whitcomb Exhibition booth Australian National University College of Science Kimberley Heenan (left), Lachlan McCalman (right) Exhibition booth Australian National University College of Science Kimberley Heenan (left), Lachlan McCalman (right) Exhibition booth Springer Exhibition booth Springer Exhibition booth GR19 Mexico City Exhibition booth GR19 Mexico City Amaldi7 posters Amaldi7 posters Participants gather before Kip Thorne's public lecture Participants gather before Kip Thorne's public lecture Participants gather before Kip Thorne's public lecture Participants gather before Kip Thorne's public lecture Entering auditorium for Kip Thorne's public lecture Entering auditorium for Kip Thorne's public lecture Public lecture by Kip Thorne Public lecture by Kip Thorne Public lecture by Kip Thorne Public lecture by Kip Thorne Kip Thorne public lecture Kip Thorne public lecture Kip Thorne public lecture Kip Thorne public lecture Roger Penrose (left), Adam Spencer (right) Roger Penrose (left), Adam Spencer (right) From left to right: John Steele, Susan Scott, Roger Penrose, David McClelland, John Webb, Adam Spencer From left to right: John Steele, Susan Scott, Roger Penrose, David McClelland, John Webb, Adam Spencer Opening of Roger Penrose's public lecture from left to right: John Webb, Adam Spencer, Roger Penrose Opening of Roger Penrose's public lecture from left to right: John Webb, Adam Spencer, Roger Penrose Roger Penrose at opening of his public lecture Roger Penrose at opening of his public lecture Public lecture by Roger Penrose Public lecture by Roger Penrose Public lecture by Roger Penrose Public lecture by Roger Penrose

  11. 'Doctor' or 'darling'? Decoding the communication partner from ECoG of the anterior temporal lobe during non-experimental, real-life social interaction

    Directory of Open Access Journals (Sweden)

    Johanna eDerix

    2012-09-01

    Full Text Available Human brain processes underlying real-life social interaction in everyday situations have been difficult to study and have, until now, remained largely unknown. Here, we investigated whether electrocorticography (ECoG recorded for pre-neurosurgical diagnostics during the daily hospital life of epilepsy patients could provide a way to elucidate the neural correlates of non-experimental social interaction. We identified time periods in which patients were involved in conversations with either their respective life partners (Condition 1; C1 or attending physicians (Condition 2; C2. These two conditions can be expected to differentially involve subfunctions of social interaction which have been associated with activity in the anterior temporal lobe (ATL, including the temporal poles (TP. Therefore, we specifically focused on ECoG recordings from this brain region and investigated spectral power modulations in the alpha (8-12 Hz and theta (3-5 Hz frequency ranges, which have been previously assumed to play an important role in the processing of social interaction. We hypothesized that brain activity in this region might be sensitive to differences in the two interaction situations and tested whether these differences can be detected by single-trial decoding. Condition-specific effects in both theta and alpha bands were observed: the left and right TP exclusively showed increased power in C1 compared to C2, whereas more posterior parts of the ATL exhibited similar (C1 > C2 and also contrary (C2 > C1 effects. Single-trial decoding accuracies for classification of these effects were highly above chance. Our findings demonstrate that it is possible to study the neural correlates of human social interaction in non-experimental conditions. Decoding the identity of the communication partner and adjusting the speech output accordingly may be useful in the emerging field of brain- machine interfacing for restoration of expressive speech.

  12. Encefalitis aguda: Manifestaciones neuropsiquiátricas como expresión de infección por virus de influenza Acute encephalitis: Neuropsychiatric manifestations as expression of influenza virus infection

    Directory of Open Access Journals (Sweden)

    Noris Moreno-Flagge

    2009-01-01

    Full Text Available El objetivo fue revisar la encefalitis en niños y adolescentes, su etiología, manifestaciones clínicas, fisiopatología, métodos diagnósticos y tratamiento, enfatizando las manifestaciones neuropsiquiátricas de la encefalitis durante una epidemia de influenza. La encefalitis se considera una inflamación del sistema nervioso central (SNC que compromete el cerebro. Se manifiesta usualmente por cefaleas, fiebre y trastorno del estado de conciencia. Puede además manifestarse por convulsiones, cambios en la personalidad y manifestaciones obsesivas (síntomas neuropsiquiátricos. Las manifestaciones dependerán del tipo de virus y las células afectadas. La encefalitis puede ser causada por una gran variedad de agentes infecciosos incluyendo virus, bacterias, hongos y parásitos. Causas virales de encefalitis incluyen herpesvirus, arbovirus, rabia y enterovirus. Casos establecidos de bacterias incluyen Borrelia burgdorferi y rickettsia y el Mycoplasma neumoniae, al cual se atribuyen varios casos de encefalitis. Otros agentes como el hongo Coccidioides immitis e Histoplasma capsulatum pueden también generarla. Más de 100 agentes se han asociado a encefalitis. El diagnóstico de encefalitis constituye un reto para el clínico, y su etiología infecciosa usualmente se identifica entre el 40% al 70% de casos. El diagnóstico se hace con absoluta certeza sólo con una biopsia cerebral. La epidemiología depende de ciertos factores como la edad, la localización geográfica, la época del año, las condiciones climáticas y la inmunocompetencia del huésped. El tratamiento temprano puede disminuir el riesgo de muerte y las secuelas. Describimos cuatro pacientes con encefalitis y manifestaciones neuropsiquiátricas durante una epidemia de influenza, con el fin de alertar sobre esta asociación.The aim is to review the encephalitis in infants and adolescents as well as its etiology, clinical manifestation, epidemiology, physiopathology, diagnostic

  13. Effect of anti-glycosphingolipid monoclonal antibodies in pathogenic fungal growth and differentiation. Characterization of monoclonal antibody MEST-3 directed to Manpα1→3Manpα1→2IPC

    Directory of Open Access Journals (Sweden)

    Straus Anita H

    2010-02-01

    Full Text Available Abstract Background Studies carried out during the 1990's demonstrated the presence of fungal glycoinositol phosphorylceramides (GIPCs with unique structures, some of them showed reactivity with sera of patients with histoplasmosis, paracoccidioidomycosis or aspergillosis. It was also observed that fungal GIPCs were able to inhibit T lymphocyte proliferation "in vitro", and studies regarding the importance of these molecules to fungal survival showed that many species of fungi are vulnerable to inhibitors of sphingolipid biosynthesis. Results In this paper, we describe a detailed characterization of an IgG2a monoclonal antibody (mAb, termed MEST-3, directed to the Paracoccidioides brasiliensis glycolipid antigen Pb-2 (Manpα1→3Manpα1→2IPC. mAb MEST-3 also recognizes GIPCs bearing the same structure in other fungi. Studies performed on fungal cultures clearly showed the strong inhibitory activity of MEST-3 on differentiation and colony formation of Paracoccidioides brasiliensis, Histoplasma capsulatum and Sporothrix schenckii. Similar inhibitory results were observed when these fungi where incubated with a different mAb, which recognizes GIPCs bearing terminal residues of β-D-galactofuranose linked to mannose (mAb MEST-1. On the other hand, mAb MEST-2 specifically directed to fungal glucosylceramide (GlcCer was able to promote only a weak inhibition on fungal differentiation and colony formation. Conclusions These results strongly suggest that mAbs directed to specific glycosphingolipids are able to interfere on fungal growth and differentiation. Thus, studies on surface distribution of GIPCs in yeast and mycelium forms of fungi may yield valuable information regarding the relevance of glycosphingolipids in processes of fungal growth, morphological transition and infectivity.

  14. Cellular and Molecular Defects Underlying Invasive Fungal Infections—Revelations from Endemic Mycoses

    Directory of Open Access Journals (Sweden)

    Pamela P. Lee

    2017-06-01

    Full Text Available The global burden of fungal diseases has been increasing, as a result of the expanding number of susceptible individuals including people living with human immunodeficiency virus (HIV, hematopoietic stem cell or organ transplant recipients, patients with malignancies or immunological conditions receiving immunosuppressive treatment, premature neonates, and the elderly. Opportunistic fungal pathogens such as Aspergillus, Candida, Cryptococcus, Rhizopus, and Pneumocystis jiroveci are distributed worldwide and constitute the majority of invasive fungal infections (IFIs. Dimorphic fungi such as Histoplasma capsulatum, Coccidioides spp., Paracoccidioides spp., Blastomyces dermatiditis, Sporothrix schenckii, Talaromyces (Penicillium marneffei, and Emmonsia spp. are geographically restricted to their respective habitats and cause endemic mycoses. Disseminated histoplasmosis, coccidioidomycosis, and T. marneffei infection are recognized as acquired immunodeficiency syndrome (AIDS-defining conditions, while the rest also cause high rate of morbidities and mortalities in patients with HIV infection and other immunocompromised conditions. In the past decade, a growing number of monogenic immunodeficiency disorders causing increased susceptibility to fungal infections have been discovered. In particular, defects of the IL-12/IFN-γ pathway and T-helper 17-mediated response are associated with increased susceptibility to endemic mycoses. In this review, we put together the various forms of endemic mycoses on the map and take a journey around the world to examine how cellular and molecular defects of the immune system predispose to invasive endemic fungal infections, including primary immunodeficiencies, individuals with autoantibodies against interferon-γ, and those receiving biologic response modifiers. Though rare, these conditions provide importance insights to host defense mechanisms against endemic fungi, which can only be appreciated in unique

  15. Disseminated cutaneous histoplasmosis with laryngeal involvement in a setting of immune reconstitution inflammatory syndrome

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    Mohamed F. Sacoor

    2017-01-01

    Full Text Available Introduction: Histoplasmosis is a systemic mycosis caused by the dimorphic fungus Histoplasma capsulatum. We report a case of disseminated cutaneous histoplasmosis with mucocutaneous involvement in an AIDS patient paradigmatic of the multifaceted nature of the disease, which is an expression of the immune reconstitution inflammatory syndrome (IRIS.Patient presentation: A 39-year-old man presented with a three month history of asymptomatic papules and nodules with necrotic centres involving the centrofacial region. The patient was diagnosed as being HIV-positive a month earlier and was commenced on antiretroviral treatment. Two weeks after the development of skin lesions, the patient complained of a sore throat and hoarseness of his voice. A fibre-optic laryngoscopy and biopsies of the skin, larynx and liver were performed.Management and outcome: The CD4 counts increased from 2 cells/µL to 124 cells/µL, whereas the viral load decreased from one million to less than 20 copies/mL. A fibre-optic laryngoscopy revealed a supraglottitis with ulceration on the epiglottis. Histology of the liver, larynx and sections of the skin demonstrated pandermal necrotising granulomatous inflammation. Grocott-Gomori methenamine silver and Periodic acid–Schiff (PAS stains revealed a relative paucity of intracellular, narrow-neck budding fungal organisms. Culture findings confirmed the diagnosis of histoplasmosis. The patient was treated with intravenous amphotericin B for two weeks followed by oral itraconazole 100 mg twice a day, with an excellent response to treatment.Conclusion: We present this case to remind clinicians that disseminated histoplasmosis in AIDS patients may occur as an expression of IRIS. A sudden onset of hoarseness with cutaneous lesions in a patient with disseminated disease should alert one to possible laryngeal histoplasmosis. Prompt recognition and treatment will avert the potential fatal complications of this disease.

  16. Identification of fungemia agents using the polymerase chain reaction and restriction fragment length polymorphism analysis

    Directory of Open Access Journals (Sweden)

    M.S. Santos

    2010-08-01

    Full Text Available Prompt and specific identification of fungemia agents is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time-consuming and not without errors. The aim of the present study was to identify the following fungemia agents: Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, Cryptococcus neoformans, Cryptococcus gattii, and Histoplasma capsulatum using the polymerase chain reaction and restriction fragment length polymorphism analysis (PCR/RFLP. More specifically: a to evaluate 3 different amplification regions, b to investigate 3 different restriction enzymes, and c to use the best PCR/RFLP procedure to indentify 60 fungemia agents from a culture collection. All 3 pairs of primers (ITS1/ITS4, NL4/ITS5 and Primer1/Primer2 were able to amplify DNA from the reference strains. However, the size of these PCR products did not permit the identification of all the species studied. Three restriction enzymes were used to digest the PCR products: HaeIII, Ddel and Bfal. Among the combinations of pairs of primers and restriction enzymes, only one (primer pair NL4/ITS5 and restriction enzyme Ddel produced a specific RFLP pattern for each microorganism studied. Sixty cultures of fungemia agents (selected from the culture collection of Fundação de Medicina Tropical do Amazonas - FMTAM were correctly identified by PCR/RFLP using the prime pair NL4/ITS5 and Ddel. We conclude that the method proved to be both simple and reproducible, and may offer potential advantages over phenotyping methods.

  17. Immune Recognition of Fungal Polysaccharides

    Directory of Open Access Journals (Sweden)

    Brendan D. Snarr

    2017-08-01

    Full Text Available The incidence of fungal infections has dramatically increased in recent years, in large part due to increased use of immunosuppressive medications, as well as aggressive medical and surgical interventions that compromise natural skin and mucosal barriers. There are relatively few currently licensed antifungal drugs, and rising resistance to these agents has led to interest in the development of novel preventative and therapeutic strategies targeting these devastating infections. One approach to combat fungal infections is to augment the host immune response towards these organisms. The polysaccharide-rich cell wall is the initial point of contact between fungi and the host immune system, and therefore, represents an important target for immunotherapeutic approaches. This review highlights the advances made in our understanding of the mechanisms by which the immune system recognizes and interacts with exopolysaccharides produced by four of the most common fungal pathogens: Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, and Histoplasma capsulatum. Work to date suggests that inner cell wall polysaccharides that play an important structural role are the most conserved across diverse members of the fungal kingdom, and elicit the strongest innate immune responses. The immune system senses these carbohydrates through receptors, such as lectins and complement proteins. In contrast, a greater diversity of polysaccharides is found within the outer cell walls of pathogenic fungi. These glycans play an important role in immune evasion, and can even induce anti-inflammatory host responses. Further study of the complex interactions between the host immune system and the fungal polysaccharides will be necessary to develop more effective therapeutic strategies, as well as to explore the use of immunosuppressive polysaccharides as therapeutic agents to modulate inflammation.

  18. Estudio histopatológico de 19 biopsias cutáneas de pacientes con sida e histoplasmosis diseminada

    Directory of Open Access Journals (Sweden)

    Gerzaín Rodríguez

    2001-06-01

    Full Text Available La histoplasmosis diseminada (HD compromete la piel y la mucosa oronasofaríngea con alta frecuencia. Entre 1.800 enfermos con sida, 19 presentaron HD, todos con compromiso cutáneo o mucocutáneo. Las lesiones cutáneas fueron máculas, pápulas umbilicadas o escamocostrosas, nódulos y úlceras, en tanto que 42% de los pacientes presentaron ulceras orales. Las biopsias mostraron dermatitis perivasculares superficiales y profundas, nodulares y difusas, foliculitis pustulosa o vasculitis con PMN y leucocitoclasia, con cantidades variables del hongo, fagocitado por macrófagos. En 5 biopsias, Histoplasma ca~sulatums e vio dentro de los nervios cutáneos que estaban rodeados de inflamación variable o tenían perinervio desflecado y estaban invadidos por macrófagos con el hongo. Los principales diagnósticos diferenciales histológicos son: vasculitis leucocitoclásica, criptococosis, aftas, paracoccidioidomicosis y leishmaniasis difusa. Los clínicos, laboratoristas y patólogos deben tener en mente la HD en toda úlcera oral o erupción máculo-papular cutánea de pacientes con sida, pues el diagnóstico rápido de la micosis conduce a un tratamiento oportuno y eficaz, que controla la enfermedad. La biopsia es un procedimiento de diagnóstico rápido, seguro y confiable. H. capsulatum es capaz de invadir los nervios cutáneos.

  19. Isolation, purification, and radiolabeling of a novel 120-kD surface protein on Blastomyces dermatitidis yeasts to detect antibody in infected patients

    Energy Technology Data Exchange (ETDEWEB)

    Klein, B.S.; Jones, J.M.

    1990-01-01

    No well-defined Blastomyces-specific antigens are currently available. We used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting to identify immunologically active molecules in the cell wall of B. dermatitidis. A major immunoreactive 120-kD protein (WI-1) was present in all five strains studied and comprised 5% of the protein in the cell wall extract obtained after freezing and thawing yeast cells. WI-1 was recognized by serum from all 10 patients with blastomycosis but by none of those from 5 patients with histoplasmosis. It was purified by electroelution, radiolabeled with 125I, and incorporated into a radioimmunoassay (RIA) for serodiagnosis of blastomycosis. Antibody to WI-1 was detected in 58 (85%) of 68 patients with blastomycosis (geometric mean titer, 1:2,981), in two (3%) of 73 patients with histoplasmosis, coccidioidomycosis, sporotrichosis, or candidiasis (titers, 1:86 and 1:91) and in none of 44 healthy persons. WI-1 was shown to be a surface molecule abundant on B. dermatitidis yeasts that were indirectly stained with serum from a rabbit immunized with WI-1. Approximately 0.93 pg of WI-1 or 4.7 x 10(6) WI-1 molecules were found on the surface of an individual yeast using an antigen-inhibition RIA; none was found on Histoplasma capsulatum or Candida albicans yeasts. We conclude that WI-1 is a novel, immunologically active surface molecule on the invasive form of B. dermatitidis and that WI-1 can be used to reliably detect antibody and study the immunopathogenesis of blastomycosis.

  20. In situ hybridization for Coccidioides immitis 5.8S ribosomal RNA sequences in formalin-fixed, paraffin-embedded pulmonary specimens using a locked nucleic acid probe: a rapid means for identification in tissue sections.

    Science.gov (United States)

    Montone, Kathleen T; Litzky, Leslie A; Feldman, Michael D; Peterman, Heather; Mathis, Benjamin; Baliff, Jeffrey; Kaiser, Larry R; Kucharczuk, John; Nachamkin, Irving

    2010-06-01

    Coccidioides immitis/Coccidioides posadasii are common causes of pulmonary infection in certain geographic areas, and are highly infectious when working with culture isolates in the laboratory. Rapid techniques to accurately identify this pathogen in tissues may be of benefit for diagnosis and in limiting the exposure of laboratory personnel to this agent. Locked nucleic acids (LNA) are modified nucleotides in which a ribonucleoside is linked between the 2'-oxygen and the 4'-carbon atoms with a methylene unit. LNA oligonucleotides exhibit increased thermal stability and make excellent probes for in situ hybridization (ISH). In this study, ISH utilizing a biotin-labeled LNA probe targeting Coccidioides sp. ribosomal RNA sequences in 6 formalin-fixed, paraffin-embedded pulmonary tissue specimens from 6 patients with culture positive or histologic findings suggestive of Coccidioides sp. infection is described. The cultures of the pulmonary specimens confirmed C. immitis in 3 of 6 patients. The ISH procedure with the LNA probe was positive in all 6 cases, although the number of organisms that were highlighted varied from rare to numerous. ISH with a biotin-labeled DNA probe of the same sequence was positive in 4 of the 6 cases and the signal intensity and number of organisms was much less than that observed with the LNA probe. Negative control tissues containing a variety of different fungal pathogens including Aspergillus sp., Fusarium sp., Blastomyces dermatitidis, Candida sp, Histoplasma capsulatum, and Zygomyces did not hybridize with the LNA and DNA probes. ISH with an LNA oligonucleotide probe targeting Coccidioides sp. ribosomal RNA is useful for rapid ISH. ISH could be rapidly performed when fungal pathogens are observed in tissue but cultures are negative or have not been performed.

  1. [Spinal cord histoplasmoma. A case report].

    Science.gov (United States)

    Rivierez, M; Heyman, D; Brebion, A; Landau-Ossondo, M; Desbois, N; Vally, P

    2002-02-01

    Over a period of 2 months, a 60-year-old man, a chicken breeder, experienced low back pain, lower limb weakness predominant on the right side, and urinary difficulties, leading progressively to a flaccid paraplegia with sphincter impairment. Concomitant poor cognitive performances were noted. MRI showed enlargement of the conus terminalis, with a low-intensity signal on T1-weighted images, high-intensity signal on T2-weighted images, and areas of intramedullar contrast enhancement. A biopsy of the lesion showed macrophages containing yeast cells, with PAS and Grocott staining aspects compatible with the presence of Histoplasma capsulatum (Hc). A brain MRI showed multiple localizations in the brain stem and in both hemispheres with associated edema. Disseminated histoplasmosis was confirmed by a biopsy of a sub-maxillary ganglion demonstrating a necrotic tuberculoid lymphadenitis containing yeast cells resembling Hc. Immune tests disclosed the presence of HTLV1 anti-bodies without immunodeficiency nor HIV co-infection. An anti-micotic treatment was started 2 weeks after surgery, with intra-venous amphotericin B, for 21 days, followed by itraconazole, orally for 90 days. Cognitive functions improved significantly in 5 weeks while paraplegia and sphincter impairment remained unchanged. Seven months later, cerebral MR aspects dramatically improved while the conus medullaris lesion diminished, and the edematous component disappeared in all areas. Even though histoplasmosis is endemic in our region, CNS localization is rare, generally in disseminated forms associated with immunodeficiency. Brain granulomas are well-known, but spinal cord histoplasmomas are exceptional: only four cases have been evaluated by MRI. Unlike our case, spinal cord forms generally improve, due to surgery associated with antifungus medication, or sometimes due to specific medical treatment alone but with sufficient dosage.

  2. Microbiological basis of oral infections and sensitivity to antibiotics.

    Science.gov (United States)

    Prieto-Prieto, José; Calvo, Almudena

    2004-01-01

    Because oral infections are common, the physician must understand the underlying etiology, pathogeny, and other variables that determine how these processes evolve in order to choose the most appropriate antibiotic drug. The special characteristics of the oral cavity determine the make-up of the microflora that lives there. Different anaerobic species belonging to the Peptostreptococcus, Prevotella, Fusobacterium, Gemella, and Porphyromonas genera are of particular interest, as are the aerobic species Streptococcus, Staphylococcus, and Corynebacterium. Each of these microorganisms occupies a different microniche within the oral cavity, and the prevailing balance is upset when conditions become modified as a result of illness or due to dental interventions such as tooth extraction or tooth scaling and polishing. Pathogenic or opportunistic bacteria (Actinomyces, Prevotella intermedia species, etc.) can develop in these conditions, as can yeasts (Candida sp., Histoplasma capsulatum), virus (herpes simplex, papilomavirus), and parasites (Entamoeba gingivalis, Trichomonas tenax). When infection occurs, the patients s immune system reacts by means of inborn immunity (non-specific) and acquired immunity (specific). Empirical treatment is administered that should be based on etiological data and on the antimicrobial sensitivity of the pathogen that is causing the infection. However, oral microflora sensitivity to different antibiotics is currently declining and there is a noticeable trend towards resistances. As a consequence of all this, the treatment of oral infections must also aim to restore the ecological balance of the oral cavity and to minimize the emergence of resistance in the microorganisms present in the mouth. Hence, epidemiological oral pathogen sensitivity studies must be conducted, fostering the administration of appropriate antibiotics at proper doses and keeping specialists abreast of the latest trends. In recent decades, oral infections comprise one of

  3. Histoplasmose do sistema nervoso central: estudo do líquido cefalorraqueano em 8 pacientes

    Directory of Open Access Journals (Sweden)

    J.A. Livramento

    1993-03-01

    Full Text Available Foram estudadas 113 amostras de LCR de 8 pacientes no período compreendido entre setembro-1980 e agosto-1992. Todos os pacientes apresentavam quadro clínico e do LCR compatível a processo meningoencefalitico de evolução protraída. Nenhum deles apresentava a síndrome de imunodeficiência adquirida. Em todos foi feito o diagnóstico de histoplasmose do SNC; em todos foram detectados anticorpos a Histoplasma capsulatum no LCR; em um foi isolada a levedura por cultura em meio de Sabouraud. As principais características do LCR por ocasião do diagnóstico foram: pleocitose moderada com predomínio de células lin-fomononucleadas porém com presença de neutrófilos e por vezes eosinófilos; hiperproteinor-raquia moderada; hipoglicorraquia; aumento moderado do teor de globulinas gama. Os pacientes foram acompanhados durante períodos que variaram de 7 a 102 meses e submetidos a exames periódicos de LCR, em função da sintomatologia clínica. O número de células do LCR e a concentração de proteínas totais apresentaram evolução caracterizada pela ocorrência de episódios de exacerbação com perfil parcialmente dissociado, favorecendo as proteínas. As concentrações de glicose eram moderadamente baixas sendo os menores valores coincidentes aos períodos de exarcebação do número de células. Os teores de globulinas gama apresentaram também oscilações, porém menos evidentes. Submetidos os pacientes a tratamento eficaz, ocorreu no LCR: rápida diminuição do número de células; aumento da taxa de glicose; lento decréscimo dos aumentos de proteínas e de globulinas gama.

  4. Comparative genomics allowed the identification of drug targets against human fungal pathogens

    Directory of Open Access Journals (Sweden)

    Martins Natalia F

    2011-01-01

    Full Text Available Abstract Background The prevalence of invasive fungal infections (IFIs has increased steadily worldwide in the last few decades. Particularly, there has been a global rise in the number of infections among immunosuppressed people. These patients present severe clinical forms of the infections, which are commonly fatal, and they are more susceptible to opportunistic fungal infections than non-immunocompromised people. IFIs have historically been associated with high morbidity and mortality, partly because of the limitations of available antifungal therapies, including side effects, toxicities, drug interactions and antifungal resistance. Thus, the search for alternative therapies and/or the development of more specific drugs is a challenge that needs to be met. Genomics has created new ways of examining genes, which open new strategies for drug development and control of human diseases. Results In silico analyses and manual mining selected initially 57 potential drug targets, based on 55 genes experimentally confirmed as essential for Candida albicans or Aspergillus fumigatus and other 2 genes (kre2 and erg6 relevant for fungal survival within the host. Orthologs for those 57 potential targets were also identified in eight human fungal pathogens (C. albicans, A. fumigatus, Blastomyces dermatitidis, Paracoccidioides brasiliensis, Paracoccidioides lutzii, Coccidioides immitis, Cryptococcus neoformans and Histoplasma capsulatum. Of those, 10 genes were present in all pathogenic fungi analyzed and absent in the human genome. We focused on four candidates: trr1 that encodes for thioredoxin reductase, rim8 that encodes for a protein involved in the proteolytic activation of a transcriptional factor in response to alkaline pH, kre2 that encodes for α-1,2-mannosyltransferase and erg6 that encodes for Δ(24-sterol C-methyltransferase. Conclusions Our data show that the comparative genomics analysis of eight fungal pathogens enabled the identification of

  5. 27 CFR 9.177 - Alexandria Lakes.

    Science.gov (United States)

    2010-04-01

    ... and Lake Darling at benchmark (BM) 1366, which is an unmarked bridge on County Road 11, known as the Carlos-Darling Bridge. From this point the boundary line continues— (2) Along the Carlos-Darling bridge... road known as County Road 38; then (4) North along County Road 38 until it intersects with an unlabeled...

  6. Disease: H01062 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H01062 Histoplasmosis Histoplasmosis is a disease caused by the fungus Histoplasma ...rtality rate of 20%. Most affected patients (70-80%) present with pneumonitis, and sometimes the disea...se may be disseminated from the lungs to other organs. Infectious disease Histoplasma c

  7. Deteccion de amastigotas en Leishmaniasis cutanea y mucocutanea por el metodo de inmunoperoxidasa, usando anticuerpo policlonal: sensibilidad y especificidad comparadas con metodos convencionales de diagnostico Detection of amastigotes in cuteneous and mucocutaneous leishmaniasis by the immunoperoxidase method, using polyclonal antibody sensibility and specifity compared with conventional diagnosis methods

    Directory of Open Access Journals (Sweden)

    Graciela Salinas

    1989-03-01

    was evaluated in 265 biopsies with the purpose of increasing the sensitivity of the diagnostic histopathology of tegumentary lesions caused by subspecies of the Leishmania braziliensis complex. A diagnosis of leishmaniasis was established by parasitological methods (181 or clinical criteria (12 in 193 patients (72.8%. In the latter group of confirmed cases standard histochemstry and immunoperoxidase were compared with direct examination of tissue scraping and culture of lesion aspirates. The detection and localization of amastigotes was more efficient using the immunoperoxidase method (61.3% than conventional histopathology with hematoxilin and eosin (34.6% or direct examination of tissue scraping (43.9%. Howerver, culture of lesion aspirates was the most sensitive procedure (89.8%. The efficiency of the immunoperoxidase method was greater in recent lesions, being positive in 75% of cases with less than 3 months evolution, while 55.6%, 37.5% and 21.1% of cases with lesion evolution of 3-5.9, 6-11, and 12 months or greater, respectively, were positive. The combined use of the direct examination of lesion scraping and immunoperoxidase applied to histological sections of the biopsy from the lesion border allowed an etiologic diagnosis of 72% of confirmed cases. Cross-reactivity was observed with Paracoccidioides braziliensis but not with Mycobacterium leprae, Sporothrix schenckii, or Histoplasma capsulatum.

  8. HIV INFECTION AND THE KIDNEY CLINICAL

    African Journals Online (AJOL)

    2008-04-04

    Apr 4, 2008 ... Viruses: herpes, cytomegalovirus, varicella zoster virus, parvovirus. Fungi: aspergillus, cryptococcus, histoplasma, candida, mucormycosis. Parasites: pneumocystis, toxoplasma, microsporidia. ▫ Glomerulopathies. HIV-associated nephropathy (HIVAN)/HIV-associated immune complex disease (HIV-IC)*.

  9. Antigen Concentrations as an Indicator of Clinical Remission and Disease Relapse in Cats with Histoplasmosis

    OpenAIRE

    Hanzlicek, A.S.; Meinkoth, J.H.; Renschler, J.S.; Goad, C.; Wheat, L.J.

    2016-01-01

    Background Treatment monitoring is subjective and disease relapse is common in cats with histoplasmosis. The Histoplasma antigen enzyme immunoassay (EIA) is a noninvasive test used for determining disease remission and detecting disease relapse in humans with histoplasmosis. The utility of the antigen EIA for these purposes in cats remains unknown. Hypothesis/Objectives Those Histoplasma antigen concentrations in urine and serum would decline with antifungal treatment and that antigen elimina...

  10. Testing Gone Amok: Leave No Teacher Candidate behind

    Science.gov (United States)

    Wepner, Shelley B.

    2006-01-01

    Teacher preparation, now acknowledged for its impact on K-12 student achievement (Darling-Hammond, 2000; Darling-Hammond, Berry, & Thoreson, 2000), needs to be part of the discussions about ways to address emerging issues with testing and accountability. What then do teacher educators who work with those preparing to teach do to guide their…

  11. Siim Nestor soovitab : Tallinn Doom Night vol.1. Odessa Pop / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2006-01-01

    Soome doom-metal ansambel Reverend Bizarre üritusel "Tallinn Doom Night vol.1" 8. dets. klubis Rockstar's. Indipoppi viljelev Rootsi duo My Darling Yoy! üritusel "Odessa Pop" 9. dets. Tallinnas klubis KuKu

  12. 78 FR 2986 - Northern Indiana Public Service Company; Notice of Application for Temporary Variance of License...

    Science.gov (United States)

    2013-01-15

    ... Energy Regulatory Commission Northern Indiana Public Service Company; Notice of Application for Temporary...-056. c. Date Filed: November 28, 2012. d. Applicant: Northern Indiana Public Service Company (licensee.... Justin Darling, Hydro Supervisor-- Chemical and Environmental Compliance, Northern Indiana Public Service...

  13. Täna kell 20 esitletakse EKA galerii...

    Index Scriptorium Estoniae

    2004-01-01

    Esitletakse norra kunstnike (loetletud) videosid "Darling, don't leave me all by myself". Korraldaja Bergeni kuraatoriduo Rakett (Asa Lövgren, Karolin Tampere). 4. IV näeb Tallinna projekti Von Krahli teatris

  14. Plaadid / Margit Tõnson

    Index Scriptorium Estoniae

    Tõnson, Margit, 1978-

    2004-01-01

    Uutest heliplaatidest Travis "Singles", Talking Heads "The Best Of", The Hidden Cameras "Mississauga Goddam", Aberfeldy "Young Forever", The Music "Welcome To The North", Bitch Alert "Kill Your Darlings", Manic Street Preachers "Lifeblood", Depeche Mode "Remixes81-04"

  15. Hiiglaslik andmekadu kõigutab Briti valitsust / Liisi Poll

    Index Scriptorium Estoniae

    Poll, Liisi, 1980-

    2007-01-01

    Rahandusminister Alistair Darling teatas parlamendile, et riigis läksid kaduma ametniku hooletuse tõttu poolte riigi perede nimed, aadressid ja kohati ka pangaandmed. Peaminister Gordon Browni ja meedia reageeringutest skandaalile. Lisa: Salainfo kaotsiminek

  16. How should agriculture adapt to worrying but uncertain information from climate science?

    Energy Technology Data Exchange (ETDEWEB)

    Hayman, P. [Climate Change Risk and Vulnerability, Allen Consulting Group, Melbourne (Australia)

    2006-02-15

    Overview of the impacts, adaptive capacity and vulnerability at crop, farm, industry and community level in the agricultural sector of the Murray-Darling basin and other key Australian agricultural areas.

  17. An Expert Instructor's Use of Social Congruence, Cognitive Congruence, and Expertise in an Online Case-Based Instructional Design Course

    National Research Council Canada - National Science Library

    Koehler, Adrie A; Kim, WooRi; Watson, Sunnie Lee; Rico, Rudy; Ertmer, Peggy

    2017-01-01

    ...—is a skill that eludes many instructors (Darling-Hammond, 2008; Ge, Yamashiro, & Lee, 2000). This study explored the role and strategies of an expert instructor in an online advanced instructional design...

  18. North Dakota Refuges : Narrative Reports of John H. Steenis : October 1937 - October 1938

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report cover monthly activities during October 1937 to October 1938 for Upper Souris, Lower Souris, Lake Darling, Des Lacs, Medicine Lake and Lostwood...

  19. 78 FR 10615 - Westfield Water Resources Department; Notice of Application Accepted for Filing and Soliciting...

    Science.gov (United States)

    2013-02-14

    ... Energy Regulatory Commission Westfield Water Resources Department; Notice of Application Accepted for.... Date filed: January 22, 2013. d. Applicant: Westfield Water Resources Department. e. Name of Project... Darling, Water Systems Engineer, Westfield Water Resources Department, 28 Sackett Street, Westfield, MA...

  20. Sobre Humanismo

    Directory of Open Access Journals (Sweden)

    Juan Zarco de Gea

    1963-04-01

    Full Text Available Nuestro tiempo vive con la conciencia de que el humanismo ha conducido al hombre al borde de la tragedia y de que, sin embargo, es menester revalorizar el humanismo, darle un sentido nuevo.

  1. Histoplasmosis presenting with solitary pulmonary nodule: Two ...

    African Journals Online (AJOL)

    2014-08-04

    Aug 4, 2014 ... acid–Schiff (PAS) revealed numerous uniform oval 1‑ to. 5‑μm PAS‑stained organisms, morphologically consistent with H. capsulatum [Figure 2]. Serological tests and culture. Histoplasmosis presenting with solitary pulmonary nodule: Two cases mimicking pulmonary metastases. C Ye, G Zhang, J Wang1, ...

  2. Resource availability and competition shape the evolution of survival and growth ability in a bacterial community.

    Directory of Open Access Journals (Sweden)

    Minna Pekkonen

    Full Text Available Resource availability is one of the main factors determining the ecological dynamics of populations or species. Fluctuations in resource availability can increase or decrease the intensity of resource competition. Resource availability and competition can also cause evolutionary changes in life-history traits. We studied how community structure and resource fluctuations affect the evolution of fitness related traits using a two-species bacterial model system. Replicated populations of Serratia marcescens (copiotroph and Novosphingobium capsulatum (oligotroph were reared alone or together in environments with intergenerational, pulsed resource renewal. The comparison of ancestral and evolved bacterial clones with 1 or 13 weeks history in pulsed resource environment revealed species-specific changes in life-history traits. Co-evolution with S. marcescens caused N. capsulatum clones to grow faster. The evolved S. marcescens clones had higher survival and slower growth rate then their ancestor. The survival increased in all treatments after one week, and thereafter continued to increase only in the S. marcescens monocultures that experienced large resource pulses. Though adaptive radiation is often reported in evolution studies with bacteria, clonal variation increased only in N. capsulatum growth rate. Our results suggest that S. marcescens adapted to the resource renewal cycle whereas N. capsulatum was more affected by the interspecific competition. Our results exemplify species-specific evolutionary response to both competition and environmental variation.

  3. Cutaneous flavobacteriosis - polymorphous skin granulomas ...

    African Journals Online (AJOL)

    A case of multiple eruptive skin granulomas caused by Flavobacterium capsulatum is described. The organism was resistant or poorly sensitive to all antibiotics except carbenicillin. Cure was brought about by using maximal doses of this drug. The source of the infection could not be proved, but it dated from an orthopaedic ...

  4. HISTOPLASMIN SURVEY IN HIV-POSITIVE PATIENTS: RESULTS FROM AN ENDEMIC AREA IN NORTHEASTERN BRAZIL

    Directory of Open Access Journals (Sweden)

    Fabricia Salvador Bezerra

    2013-07-01

    Full Text Available SUMMARY Background. Disseminated histoplasmosis is common in AIDS patients with advanced immunosuppression in Ceará, Northeastern Brazil. The goal of this study was to determine the prevalence of Histoplasma infection in patients with HIV/AIDS living in Fortaleza, the capital of Ceará. Methods. Intradermal tests with histoplasmin (mycelial phase were performed in 161 HIV patients with CD4 ≥ 350 cells/mm 3 . Evidence of recent illness was evaluated with immunodiffusion (ID tests in 76 of these individuals. Results. A total of 11.8% of patients reacted to histoplasmin and 2.63% had ID test positive to Histoplasma. The presence of mango trees (Mangifera indica in the patient neighborhood (OR = 2.870; 95% CI = 1.081-7.617; p = 0.040 and past activity involving soil (OR = 2.834; 95% CI = 1.045-7.687; p = 0.045 or visits to a farm (OR = 3.869; 95% CI = 1.189-12.591; p = 0.033 were significantly associated with Histoplasma infection. Conclusions. Patients with HIV living in Fortaleza have an expressive prevalence of infection with Histoplasma.

  5. MicroRNA-200c: A Novel Way to Attack Breast Cancer Metastases by Restoring the Epithelial Phenotype

    Science.gov (United States)

    2012-02-01

    Vannier C, Darling D, zur Hausen A, Brunton VG, Morton J, Sansom O, Schüler J, Stemmler MP, Herzberger C, Hopt U, Keck T, Brabletz S, Brabletz T: The...F, Sonntag A, Waldvogel B, Vannier C, Darling D, zur Hausen A, Brunton VG, Morton J, Sansom O, Schüler J, Stemmler MP, Herzberger C, Hopt U, Keck T...Ann Surg Oncol. 2007;14(12):3629–37. 117. Frederick BA, Helfrich BA, Coldren CD, Zheng D, Chan D, Bunn Jr PA, et al. Epithelial to mesenchymal

  6. Multiparameter probability distributions for heavy rainfall modeling in extreme southern Brazil

    Directory of Open Access Journals (Sweden)

    Samuel Beskow

    2015-09-01

    New hydrological insights for the region: The Anderson–Darling and Filliben tests were the most restrictive in this study. Based on the Anderson–Darling test, it was found that the Kappa distribution presented the best performance, followed by the GEV. This finding provides evidence that these multiparameter distributions result, for the region of study, in greater accuracy for the generation of intensity–duration–frequency curves and the prediction of peak streamflows and design hydrographs. As a result, this finding can support the design of hydraulic structures and flood management in river basins.

  7. Hemophagocytosis in a patient with chronic lymphocytic leukemia and histoplasmosis.

    Science.gov (United States)

    Rao, Ravi D; Morice, William G; Phyliky, Robert L

    2002-03-01

    We present a case of hemophagocytosis in the setting of a disseminated Histoplasma infection in a patient with B-cell chronic lymphocytic leukemia (CLL). A 68-year-old man with CLL presented with progressive pancytopenia and fevers after therapy with cyclophosphamide and fludarabine phosphate. Extensive evaluation for a source of infection revealed a pulmonary nodule. A biopsy specimen taken from the nodule showed granulomas containing Histoplasma organisms. A bone marrow biopsy specimen demonstrated disseminated histoplasmosis and intense hemophagocytosis. Antifungal therapy with amphotericin B was initiated, and the fevers and cytopenias resolved. Hemophagocytic syndrome is an uncommon condition with many origins. It is characterized by a proliferation of histiocytes with phagocytosis of formed elements of blood. Clinical manifestations include signs and symptoms of immune activation and decreased peripheral blood cell counts. This condition is often underdiagnosed because clinicians are unfamiliar with it.

  8. Three Phoma spp. synthesised novel silver nanoparticles that possess excellent antimicrobial efficacy.

    Science.gov (United States)

    Rai, Mahendra; Ingle, Avinash P; Gade, Aniket K; Duarte, Marta Cristina Teixeira; Duran, Nelson

    2015-10-01

    The authors report extracellular mycosynthesis of silver nanoparticles (AgNPs) by Phoma capsulatum, Phoma putaminum and Phoma citri. The AgNPs thus synthesised were characterised by UV-visible spectrophotometer, Fourier transform infrared spectroscopy, Nanosight LM20 and transmission electron microscopy, which confirmed the synthesis of mostly spherical and polydisperse nanoparticles capped with proteins. The size of AgNPs was found in the range of 10-80 , 5-80 and 5-90 nm with an average size of 31.85, 25.43 and 23.29 nm by P. capsulatum, P. putaminum and P. citri, respectively. Further, potential antimicrobial activity was reported against Aspergillus niger, Candida albicans, Salmonella choleraesuis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The lowest minimal inhibitory concentration (MIC) (0.85 µg/ml) was reported for AgNPs synthesised from P. citri against S. choleraesuis. However, AgNPs synthesised from P. capsulatum showed the highest MIC (10.62 µg/ml) against S. choleraesuis, P. aeruginosa and E. coli (clinical isolate). The same MIC values (10.62 µg/ml) were also reported against P. aeruginosa and both clinical and standard isolates of E. coli for AgNPs synthesised from P. citri. It was also observed that all the silver nanoparticles showed remarkable antifungal and antibacterial activity against these tested pathogens as compared with the commercially available antifungal and antibacterial agents.

  9. Planktonic algae and cyanoprokaryotes as indicators of ecosystem ...

    African Journals Online (AJOL)

    2013-10-07

    Oct 7, 2013 ... However, results also show a change from a Chrysophyceae-dominated community to one dominated by. Bacillariophyceae. Increased ... as well as the underground located groundwater aquifers and springs in the karst landscape ...... Murray-Darling. Basin Commission, Canberra City, Australia. 307 pp.

  10. Characterization of MTNR1A gene in terms of genetic variability in a ...

    Indian Academy of Sciences (India)

    2015-12-07

    Dec 7, 2015 ... have been associated with seasonal reproductive pattern in sheep breeds like Merino d'arles (Pelletier et al. 2000), small tail Han sheep (Chu et al. 2006), Awasi (Faigl et al. 2008),. Sarda (Carcangiu et al. 2009) and Dorset (Mateescu et al. Keywords. MTNR1A gene; seasonality; genotype; polymorphism.

  11. Plaadid : Pop & rock. Ameerika. Klassika

    Index Scriptorium Estoniae

    2002-01-01

    Uutest heliplaatidest. Kid Loco "Kill Your Darlings", Creed "Weathered", Cranberries "Wake Up and Smell the Coffee", "America - A Tribute To Heroes", "The Concert For New York City", "God Bless America", Michael Nyman "The Very Best of Film Music 1980-2001". Eesti plaadid : Dave Benton "From Monday to Sunday", 2 Quick Start "Ühena miljoneist"

  12. Engineering Ferroic and Multiferroic Materials for Active Cooling Applications

    Science.gov (United States)

    2014-02-11

    Ferromagnetic Metallic Manga - nites. J. Phys.: Condens. Matter 2007, 19, 315210. 43. Adamo, C.; Ke, X.; Wang, H. Q.; Xin, H. L.; Heeg, T.; Hawley, M. E...A. J.; Darling, T.; Migliori, A. Quantifying Strain Dependence in “Colossal” Magnetoresistance Manga - nites. J. Appl. Phys. 1998, 83, 1588. 48. Cao

  13. The Importance of the School Principal. Research Brief

    Science.gov (United States)

    Williamson, Ronald

    2011-01-01

    Research shows that effective leadership is essential in schools. When schools have effective principals they are far more likely to be effective and to positively impact student achievement (Davis, Darling-Hammond, LaPointe & Meyerson, 2005; Firestone & Riehl, 2005; Marzano, Waters & McNulty, 2005). Researchers have identified a set of leadership…

  14. Legislación y políticas sociales dos problemas de salud en el logro de la equidad

    Directory of Open Access Journals (Sweden)

    Helena Mercedes González Gómez

    2003-01-01

    Full Text Available La Ley para las mujeres rurales es un sueño y una esperanza largamente luchada por las mujeres colombianas, hasta ahora solo ha sido sancionada pero no ha sido aplicada, si se reglamenta en el espíritu que las mujeres han querido darle y se aplica, no sol

  15. Wonder girls: Undercurrents of resistance in the representation of teenage girls in 1980s American cinema

    NARCIS (Netherlands)

    Wilkinson, M.C.

    2013-01-01

    This thesis explores the representation of teenage girls in 1980s American teen films such as Little Darlings (1980), Smooth Talk (1985), Just One of the Guys (1985), Pretty in Pink (1986) and Mermaids (1990). It uses film analysis and feminist film theory to subject a range of case studies to three

  16. The karyotype and taxonomie status of Cryptomys hottentotus ...

    African Journals Online (AJOL)

    Cytogenetic analyses were performed on twenty specimens referable to Cryptomys hottentotus darling! from north eastern Zimbabwe. In comparison to the southern subspecies C. h. hottentotus (aFN = 102) and C. h. natalensis (aFN = 100), C. h. darHngi showed the same diploid number (2n = 54), but a striking reduction in ...

  17. Delicious Heart-Healthy Latino Recipes

    Science.gov (United States)

    ... calorías en sus recetas preferidas. Agregue hierbas y especias a las recetas en lugar de sal para darles más sabor. Si ... saber con solo mirar . Use un termómetro de cocina para estar seguro. Temperaturas ... pollo y pato Enteros, en pedazos o molida 74°C Pla Se ti ...

  18. 07 Smith 02.pmd

    African Journals Online (AJOL)

    Owner

    23 Jun 2009 ... TYDSKRIF VIR LETTERKUNDE • 46 (2) • 2009. 97. Murder your darlings: Breytenbach, die dood en die vrou. Francois Smith. Francois Smith is 'n boekredakteur en. -joernalis van Kaapstad wat vir verskeie uitgewerye manuskripte versorg en ontwikkel. Hy was voorheen die boekeredakteur van Die Burger.

  19. Adolescents' Perceptions of Parental Goals, Practices, and Styles in Relation to Their Motivation and Achievement

    Science.gov (United States)

    Spera, Christopher

    2006-01-01

    The current study examines research questions proposed by an expanded version of Darling and Steinberg's contextual model of parenting. Using a sample of 184 adolescents, the analyses indicated that adolescents' perceptions of parental educational goals and values were related positively and significantly to their reports of parental school…

  20. Cine y psicología: las emociones en el cine de terror y la comedia en personajes trastornados

    OpenAIRE

    Cabrera Muñoz, Beatriz

    2013-01-01

    Se analiza la visión que se ha dado en el cine sobre los trastornos de la personalidad. Cómo el comunicador construye a esos personajes para darle la mayor credibilidad y realidad posible. Se repasan las diferentes estrategias empleadas en el lenguaje cinematográfico en los géneros de terror y comedia

  1. Beyond Authoritarianism: A Cultural Perspective on Asian American Parenting Practices.

    Science.gov (United States)

    Chao, Ruth K.

    A study was conducted to determine Asian American conceptualizations of parenting, focusing on socialization goals, parenting style, and parenting practices related to schooling, aspects of parental influences discussed by D. Darling and L. Steinberg (1993). It was suggested that the standard conceptualizations of parenting style, those of D.…

  2. President Obama and Education: The Possibility for Dramatic Improvements in Teaching and Learning

    Science.gov (United States)

    Darling-Hammond, Linda

    2009-01-01

    From the unique perspective gained heading Obama's education policy transition team, Darling-Hammond describes President Obama's commitment to making the education of every child a collective responsibility and reviews the major tenets of the new administration's plans for education. She reflects on the importance of suggested policy changes,…

  3. Reciprocal Relations between Perceived Parental Knowledge and Adolescent Substance Use and Delinquency: The Moderating Role of Parent-Teen Relationship Quality

    Science.gov (United States)

    Abar, Caitlin C.; Jackson, Kristina M.; Wood, Mark

    2014-01-01

    The current study prospectively examined hypothesized short- and long-term reciprocal relations between perceived parental knowledge and adolescent heavy episodic drinking, marijuana use, and delinquency. Using the contextual model of parenting style (Darling & Steinberg, 1993), we examined the extent to which the bidirectional nature of…

  4. Romanticizing Culture: The Role of Teachers' Cultural Intelligence in Working with Diversity

    Science.gov (United States)

    Molina, Sarina Chugani

    2013-01-01

    As the world is becoming increasingly flat (Ang, Van Dyne, Koh, Templer, & Chandrasekar, 2007; Darling-Hammond, 2010; Friedman, 2005), the classroom has become a mirror that often reflects this phenomenon at a microcosmic level. As such, teacher preparation programs are continuing to emphasize the importance of understanding and valuing…

  5. Grrlziner

    DEFF Research Database (Denmark)

    Ladendorf, Martina

    -feministiska strömningar som riot grrrl och annan tredje vågen-feminism. Efter en inledande textanalys av elva grrlziner studeras de svenska grrlzinerna Darling och Corky, ur både ett producent- och ett användarperspektiv, samt textanalytiskt. Fokus ligger på vilka identiteter som konstrueras av de båda tidningarna. I...

  6. Analysis of humpback whale sounds in shallow waters of the ...

    Indian Academy of Sciences (India)

    2015-04-27

    Apr 27, 2015 ... Al Robaae K 1974 Tursiops aduncus bottle nosed dolphin: a new record for Arab Gulf; with notes on Cetacea of the region. Bull. Basrah Nat. Hist. Mus. 1 7–16. Au W, Darling J and Andrews K 2001a High frequency harmonics and source level of humpback whale songs. J. Acoust. Soc. Am. 110 2770.

  7. Ueber zwei bisher verkannte Arten: Pitta angolensis, Vieill. und P. pulih, Fraser

    NARCIS (Netherlands)

    Finsch, O.

    1903-01-01

    Unter dem Namen » Pitta longipennis” beschrieb Reichenow unlängst ein von A. Holz in Ipiana bei Langenburg, an der Nordostküste des Niassa-Sees, erhaltenes Exemplar als neue Art, von der Sharpe ganz kürzlich eine gute Abbildung veröffentlichte (l. c.) nach einem Exemplare das I. Ffolliott Darling

  8. From Good to Great: Exemplary Teachers Share Perspectives on Increasing Teacher Effectiveness across the Career Continuum

    Science.gov (United States)

    Behrstock-Sherratt, Ellen; Bassett, Katherine; Olson, Derek; Jacques, Catherine

    2014-01-01

    For well over a decade, teachers have been recognized as the single most important school-level factor influencing student achievement (Darling-Hammond, 2000; McCaffrey, Lockwood, Koretz, & Hamilton, 2003; Rivkin, Hanushek, & Kain, 2000; Rowan, Correnti & Miller, 2002; Wright, Horn, & Sanders, 1997). Tremendous public resources…

  9. Global Education Reform: How Privatization and Public Investment Influence Education Outcomes

    Science.gov (United States)

    Adamson, Frank, Ed.; Astrand, Bjorn, Ed.; Darling-Hammond, Linda, Ed.

    2016-01-01

    With contributions from Linda Darling-Hammond, Michael Fullan, Pasi Sahlberg, and Martin Carnoy, "Global Education Reform" is an eye-opening analysis of national educational reforms and the types of high-achieving systems needed to serve all students equitably.The collection documents the ideologically and educationally distinctive…

  10. Dropout Factories: The Profitability of "At-Risk" Students

    Science.gov (United States)

    Eastman, Nicholas J.

    2016-01-01

    Every public school teacher today is charged, above all else, with a utilitarian form of workforce preparation: college and career readiness. The distinction between college and career is superficial. The overarching goal of both is career readiness, and STEM careers are the darlings of the "readiness" discourse. Within the STEM reform…

  11. We May Well Become Accomplices: To Rear a Generation of Spectators Is Not to Educate at All

    Science.gov (United States)

    King, Joyce E.

    2016-01-01

    Research on education and society is the focus in discussing four essays of AERA past presidents, Newton Edwards, Maxine Greene, Linda Darling-Hammond, and William F. Tate, IV. The title, "We May Well Become Accomplices... ," is taken from Greene's speech to foreground inherent moral obligations of scholars when racial and social justice…

  12. Teacher Dispositions Affecting Self-Esteem and Student Performance

    Science.gov (United States)

    Helm, Carroll

    2007-01-01

    Research supports several factors related to student success. Darling-Hammond (2000) indicated that the quality of teachers, as measured by whether the teachers were fully certified and had a major in their teaching field, was related to student performance. Measures of teacher preparation and certification were the strongest predictors of student…

  13. Progesterone profiles of postpartum dairy cows as an aid to ...

    African Journals Online (AJOL)

    mining the concentration of progesterone in milk samples. (Laing & Heap, 1971; Darling, Laing & Harkness, 1974). The rapid measurement of progesterone in milk using a semi-automated radioimmunoassay (RIA) is now a well established technique for pregnancy diagnosis in dairy cows. (Booth & Holdsworth, 1976).

  14. A comparison of changes in river runoff from multiple global and catchment-scale hydrological models under global warming scenarios of 1 °C, 2 °C and 3 °C

    NARCIS (Netherlands)

    Gosling, S.N.; Zaherpour, J.J.; Mount, N.J.; Hattermann, F.F.; Dankers, R.; Arheimer, B.; Breuer, L.; Ding, J.; Haddeland, I.; Kumar, R.; Kundu, D.; Liu, J.; van Griensven, A.; Veldkamp, T.I.E.; Vetter, T.; Wang, X.; Zhang, X.

    2016-01-01

    We present one of the first climate change impact assessments on river runoff that utilises an ensemble of global hydrological models (Glob-HMs) and an ensemble of catchment-scale hydrological models (Cat-HMs), across multiple catchments: the upper Amazon, Darling, Ganges, Lena, upper Mississippi,

  15. Distorting the Past

    Science.gov (United States)

    Klehr, Harvey

    2004-01-01

    Many historians on the left are unwilling to renounce communism and the agents in this country who ran its insidious errands. Despite damning evidence from Soviet archives, Julius and Ethel Rosenberg, Alger Hiss, and other traitors remain the darlings of many leaders of the profession. Harvey Klehr documents how, in journals and recent textbooks,…

  16. 78 FR 30872 - Marine Mammals; File Nos. 14451, 14353, and 13846

    Science.gov (United States)

    2013-05-23

    .... Researchers target numerous cetacean species including endangered blue whales (Balaenoptera musculus), fin whales (B. physalus), humpback whales (Megaptera novaeangliae), sei whales (B. borealis), and sperm... 04609; and Jim Darling, Ph.D., Whale Trust, P.O. Box 384, Tofino, BC V0R2Z0, Canada, for Scientific...

  17. 78 FR 2955 - Marine Mammals; File Nos. 14451, 14353, and 13846

    Science.gov (United States)

    2013-01-15

    ... blue whales (Balaenoptera musculus), fin whales (B. physalus), humpback whales (Megaptera novaeangliae), sei whales ] (B. borealis), and sperm whales (Physeter macrocephalus), during aerial and vessel... Darling, Ph.D., Whale Trust, P.O. Box 384, Tofino, BC V0R2Z0, Canada, have applied for amendments to...

  18. Changing the Game: What Happens when Video Games Enter the Classroom?

    Science.gov (United States)

    Squire, Kurt

    2005-01-01

    Over the past few years, games have gone from social pariahs to the darlings of the media, technology, and now educational industries. E-learning educators in particular stand to learn a lot about building next-generation learning environments from games. While online courses are usually little more than "online course notes," games offer entire…

  19. "Don't Just Talk about It; Be about It": Doing Equity Work in Mathematics Education

    Science.gov (United States)

    Jett, Christopher C.

    2012-01-01

    During the last few decades, equity concerns have become a priority for some researchers and mathematics (education) organizations. Scholars have begun to address issues of equity through a critical lens (e.g., Boaler, 2007; Darling-Hammond, 2005; Martin, 2003) in their work and scholarship. In this article, the author seeks to add to the equity…

  20. The Poor Don’t Need Another Prophet: A People-Centered Approach to Microfinance and Education in Bolivia

    NARCIS (Netherlands)

    P.A. Arora (Payal)

    2006-01-01

    textabstractIntroduction: As international aid agencies increasingly embrace microfinance, the darling of international development, questions are beginning to surface. Who benefits from this savings and lending system that claims to target the poor? Does an increase in social capital of the poor

  1. The Portable Sawmill and Other Challenges to REDD+ in Papua New Guinea

    DEFF Research Database (Denmark)

    Dalsgaard, Steffen; Pedersen, Marianne

    the failure of voluntary carbon trade due largely to scams, REDD+ has been their new darling. However, there are currently only a few pilot projects mainly in areas, without large-scale logging. This paper argues that to understand the possibility for successfully implementing REDD+, one must look...

  2. An integrated community engagement initiative in the rural town of ...

    African Journals Online (AJOL)

    ... in the specialization field of Adult Education and could serve as an example of a planned integrated approach to community engagement. The initiative was implemented in the rural community of Darling in the Western Cape Province of South Africa who experience challenges associated with poverty and unemployment.

  3. Orportunidades de Mejoramiento de la Ganaderia

    Science.gov (United States)

    Es muy comun escuchar la frase de que untoro contribuye en la produccion de becerros del ano, sin embargo es conceniente darles un mayor credito de valor en su contribucion en el conjunto de la retabilidad y productividad de un hato de cria. Ha sido estimado que la fertilidad es 10 veces mas import...

  4. Jagged-1 Signaling Pathway in Prostate Cancer Cell Growth and Angiogenesis

    Science.gov (United States)

    2010-04-01

    B.Waldvogel, C. Vannier, D. Darling, H.A. zur, V.G. Brunton, J. Morton, O. Sansom, J. Schuler, M.P. Stemmler, C. Herzberger , U. Hopt, T. Keck, S...mechanisms, markers and strategies to overcome drug resistance in the clinic, Biochim. Biophys. Acta 1796 (2009) 75–90. [66] B.A. Frederick , B.A

  5. Accountability Is More than a Test Score

    Science.gov (United States)

    Turnipseed, Stephan; Darling-Hammond, Linda

    2015-01-01

    The number one quality business leaders look for in employees is creativity and yet the U.S. education system undermines the development of the higher-order skills that promote creativity by its dogged focus on multiple-choice tests. Stephan Turnipseed and Linda DarlingHammond discuss the kind of rich accountability system that will help students…

  6. Hip-Hop Literature: The Politics, Poetics, and Power of Hip-Hop in the English Classroom

    Science.gov (United States)

    Kelly, Lauren Leigh

    2013-01-01

    There is an educational disconnect between students' individual backgrounds and the instruction that they traditionally receive in school (Darling-Hammond 3). This division is even more severe for black, Latino/Latina, and economically underprivileged students, who often lack the support, experience, or resources to fully engage in…

  7. Developing Principals as Racial Equity Leaders: A Mixed Method Study

    Science.gov (United States)

    Raskin, Candace F.; Krull, Melissa; Thatcher, Roberta

    2015-01-01

    This article will present information and research on how a college of education is intentionally developing principals to lead with confidence and racial competence. The nation's student achievement research is sobering: our current school systems widen already existing gaps between white students and students of color, (Darling-Hammond, L. 2004,…

  8. Parental Characteristics, Ecological Factors, and the Academic Achievement of African American Males

    Science.gov (United States)

    Hines, Erik M.; Holcomb-McCoy, Cheryl

    2013-01-01

    Parental characteristics, ecological factors, and the academic achievement of African American male high school students were examined. One hundred fifty-three 11th and 12th grade African American males completed the Parenting Style Index (Steinberg, Lamborn, Darling, Mounts, & Dornbusch, 1994) and a demographic questionnaire. Results…

  9. "Miss, I Am Not Being Fully Prepared": Student-Teachers' Concerns about Their Preparation at a Teacher Training Institution in Jamaica

    Science.gov (United States)

    Roofe, Carmel G.; Miller, Paul

    2013-01-01

    The issue of teacher preparation continues to occupy academic discourse relating to student outcomes and student achievement (Stronge, Ward & Grant, 2011). Research has supported the view that there is an inextricable connection between student outcomes, quality of teaching and teachers, and teacher preparation (Darling-Hammond 2005; Grover…

  10. How to be a local media celebrity.

    Science.gov (United States)

    Trent, Nancy

    2009-01-01

    Building relationships with the media in your region can turn you into a local "media darling. "As a PR-savvy doctor, there are certain techniques to get you the media attention you desire. This article will offer tips for getting local media on your side and working with media, and ways to mobilize your vendors to garner PR in your region.

  11. Professional Accountability for Improving Life, College, and Career Readiness

    Science.gov (United States)

    Snyder, Jon; Bristol, Travis J.

    2015-01-01

    This article builds on Darling-Hammond, Wilhoit, and Pittenger's (2014) new paradigm on "Accountability for College and Career Readiness" by focusing on one of its three pillars--professional accountability. The article begins by offering a conceptual framework for professional accountability for improvement. Next, it highlights slices…

  12. Vol 38 2010 Art 3.pub

    African Journals Online (AJOL)

    user

    the scholarship of application. Educational goals of developing the skills of self management, autonomy and social responsibility are fostered in the process of .... of Darling, Stellenbosch, Montagu and Paarl in the. W estern Cape Province of South Africa. It was im por- tant that these projects addressed needs in a schol-.

  13. 77 FR 36510 - Applications for New Awards: Personnel Development To Improve Services and Results for Children...

    Science.gov (United States)

    2012-06-19

    ... disabilities (Jaquith, Mindich, Wei, & Darling-Hammond, 2010; Institute of Medicine (IOM) and National Research... explicit learning outcomes related to professional practice standards, a detailed outline or narrative of... strategies. Oxford, OH: Learning Forward. Institute of Medicine (IOM) and National Research Council (NRC...

  14. Engaging Second-Stage Teachers in Their Work: The Role of Professional Culture in Schools

    Science.gov (United States)

    Kirkpatrick, Cheryl Lynne

    2009-01-01

    Educational researchers acknowledge that teachers make a difference in the educational outcomes of youth (Boyd, Lankford, Loeb, Rockoff, & Wyckoff, 2008; Darling-Hammond, 2000; Hanushek, 1992; Hanushek & Rivkin, 2007; Johnson, 2006; Sanders & Rivers, 1996; Sanders & Horn, 1998). However, staffing schools with qualified teachers has proven…

  15. Digital Adults: Beyond the Myth of the Digital Native Generation Gap

    Science.gov (United States)

    Tufts, Debra Roben

    2010-01-01

    The digital native has been the darling of market research and a major focus of education consternation throughout the first decade of the 2000s. These are the children and young adults the literature describes as those born after 1980 and who exhibit high technical savvy, particularly as it pertains to information and communication technology…

  16. Sõrmuste isand Karl Fritsch / Merike Alber

    Index Scriptorium Estoniae

    Alber, Merike

    2008-01-01

    Saksa ehtekunstniku Karl Fritschi (sünd. 1963) näitus "Metrosideros robusta. Ehted" Eesti Tarbekunsti- ja Disainimuuseumis kuni 29. VI. Karl Fritsch pälvis 2006. a. Francoise van den Boschi (hollandi ehtekunstnik, 1944-1977) auhinna, 1995. ja 2007. a. Herbert Hoffmanni preemia. Darling Publications kirjastas Karl Fritschist raamatud "Metrosideros Robusta" ja "Baby Brick"

  17. Little hope on the 'Cape of Good Hope'

    Energy Technology Data Exchange (ETDEWEB)

    Schwab, Nina

    2009-07-01

    FIFA World Cup 2010 is to be running as smoothly as possible - meaning also without power cuts. Whether this can be guaranteed is uncertain. Equally incalculable is the increased implementation of wind energy in South Africa. The example of the Darling Wind Farm illustrates that wind production at the continent's southern tip is no easy endeavour. (orig.)

  18. La identidad caribe en los 12 cuentos peregrinos.

    OpenAIRE

    Herrera Molina, Luis Carlos

    2014-01-01

    En un análisis tentativo sobre los Cuentos Peregrinos aparece una obsesión que trasciende la totalidad de estos cuentos: la identidad latinoamericana. Aspecto que queremos resaltar, para lo cual partimos de la posibilidad de darles una interpretación global.

  19. Are the Guerrillas Gone? A Historical Political Economy and Social Analysis of the Rise and Demise of the Fuerzas Armadas Revolucionarias Colombianas (FARC), 1964-2010

    Science.gov (United States)

    2009-12-01

    vida en Ingles. Casa De Narino. Retrieved June 20, 2009 from http://www.presidencia.gov.co/prensa_new/presidente/hvingles.htm Darling, J. (2000). To...2006). Dos décadas de escalamiento del conflicto armado en colombia (1986–2006) (1st ed.). Bogotá: Centro de Investigaciones y Proyectos

  20. Histoplasmose no Nordeste do Brasil. Relato de três casos** Trabalho realizado em parceria entre a Clínica de Pneumologia do Hospital Getúlio Vargas, Hospital de Ensino da UFPI na cidade de Teresina/PI e o laboratório de Micologia Médica do IPEC/ Instituto Oswaldo Cruz, Rio de Janeiro/RJ, Brasil

    Directory of Open Access Journals (Sweden)

    Antônio de Deus Filho

    2009-01-01

    Full Text Available Resumo: A histoplasmose é uma micose endémica em certas áreas dos EUA e América Latina, causada pelo fungo dimórfico geofílico H. capsulatum. A descrição de casos no Nordeste do Brasil, embora não sendo área endémica, é de rara ocorrência, indicando a existência de microfocos em várias regiões. Razões precisas para esse padrão de distribuição são desconhecidas. Actividades relacionadas com a remoção do solo em tais locais estão associadas à exposição ao H. capsulatum. O objectivo desse trabalho é descrever três casos isolados de histoplasmose ocorridos na região Nordeste do Brasil, em indivíduos expostos a distintas actividades (demolição de domicílios, escavação de poços e exposição a morcegos.Rev Port Pneumol 2009; XV (1: 109-114 Abstract: Histoplasmosis is an endemic mycosis in specific areas of the U.S. and Latin America, caused by dimorphic geophilic fungus H. capsulatum. The description of cases in Brazil’s northeast, considering that this region isn’t an endemic area, indicates the existence of small outbreaks in several regions. Precise reasons for this pattern of distribution are still unknown. Activities related to the removal of soil in these places are associated with exposure to H. capsulatum. The objective of this work is to describe three isolated cases of histoplasmosis wich occurred in Brazil’s northeast region in individuals exposed to different activities (demolition of houses, wells diggers and exposure to bats.Rev Port Pneumol 2009; XV (1: 109-114 Palavras-chave: Histoplasmose, micose sistémica, cavador de poços, demolição, morcegos, Key-words: Histoplasmosis, systemic mycosis, wells diggers, demolition, bats

  1. Linfocitopenia T CD4+ idiopática: reporte de caso

    OpenAIRE

    Pulido Arenas, Jorge; Pontificia Universidad Javeriana; Tobon Trujillo, Manuela; Garzón, Javier Ricardo

    2017-01-01

    Este artículo presenta el caso de un hombre mayor que ingresó con cuatro meses deevolución de hemiparesia izquierda progresiva con sospecha de etiología neoplásica.Se le realizó una biopsia cerebral estereotáctica que reveló lesiones granulomatosascon estructuras micóticas intracelulares compatibles con histoplasma. Adicionalmente,el paciente había requerido una orquidectomía debido a una masa testicular con unabiopsia que evidenció lesiones granulomatosas. La prueba del VIH y la carga viralf...

  2. Miguel Angel

    OpenAIRE

    Otero Álvarez, Joel

    2013-01-01

    Consiste todo en aislar la actividad pictórica y escultórica a partir de la obra de un artista capaz de darle al arte el más decisivo lugar, luego de los aportes de la pintura rupestre y del desbordante despliegue de la escultura griega antigua donde lo pétreo y lo pictórico se enlazaban sin atenuantes.

  3. Impacts of changing water price and availability on irrigated dairy farms in northern Victoria

    OpenAIRE

    Ho, Christie K.M.; Armstrong, Dan P.; Doyle, Peter T.; Malcolm, Bill

    2004-01-01

    Farming systems throughout the Murray-Darling Basin are under increasing scrutiny from the perspective of ecological sustainability of farm and catchment systems. In northern Victoria, the dairy industry is a major user of water, and contributes to the environmental issues. Changes in irrigation water price, availability and policy will invariably impact on the viability of dairy farming in this region, but the diversity of dairy farm systems suggests that the impact will vary between farms. ...

  4. Benefits of increased irrigation efficiency in the Murrumbidgee Irrigation Area

    OpenAIRE

    Kemp, Adrian; Hafi, Ahmed

    2001-01-01

    Limited water availability in the face of increasing competing demands for water; including water for environmental purposes, has highlighted the need to make the most efficient use of the available water in the Murray Darling Basin. This study forces on the Murrumbidgee Irrigation Area (MIA) and considers the benefits of increasing irrigation efficiency. A model of the MIA which incorporates both on and off farm components is used to evaluate the benefits of adopting two on-farm options-twin...

  5. Defense Labs: The Innovation Engine for Sustaining Our Quality Edge

    Science.gov (United States)

    2015-10-01

    to magnetics. • Dr. Rasha Hammamieh, U.S. Army Center for Environ- mental Health , for her work in the Post-Traumatic Stress Disorder (PTSD) Systems...U.S. Army Aeromedical Research Laboratory (USAARL) • U.S. Army Center for Environmental Health Research (USACEHR) • U.S. Army Institute of...themselves in those same roles, and it pushes them to pursue STEM fields,” says Faith Darling, the STEM Coordinator at the Bullis School in Potomac

  6. Liquid assets: The Value of North Eastern British Columbia’s Groundwater Resources in the Face of Climate Change and Competing Uses

    OpenAIRE

    Candlish-Rutherford, Coral Cree

    2016-01-01

    Northeastern British Columbia will experience future groundwater scarcity as a result of climate change and competing uses of water. The Government of British Columbia (BC) now regulates groundwater for the first time in history through the Water Sustainability Act (WSA). The WSA allocates water based on a first-in-time, first-in-right system which does not promote sustainability or efficiency.This study uses lessons learned from water management in Australia’s Murray-Darling Basin, Alberta’s...

  7. Review of world genera of Ceinae, with the description of two new Palaearctic species of Spalangiopelta Masi (Hymenoptera, Chalcidoidea, Pteromalidae

    Directory of Open Access Journals (Sweden)

    Mircea-Dan Mitroiu

    2016-12-01

    Full Text Available The paper provides the first illustrated key to all described genera of Ceinae, i.e., Bohpa Darling, 1991, Cea Walker, 1837, and Spalangiopelta Masi, 1922. Based on the study of the original material, the genus Diparisca Hedqvist, 1964 stat. nov. is removed from the synonymy with Spalangiopelta and its higher classification is discussed. Spalangiopelta rameli sp. nov. and S. viridis sp. nov. are described from Greece and the Canary Islands, respectively.

  8. RESEÑA DEL LIBRO DIDÁCTICA DE LA INVESTIGACIÓN. UNA PROPUESTA FORMATIVA PARA EL DESARROLLO DE LA CREATIVIDAD Y LA INTELIGENCIA.

    Directory of Open Access Journals (Sweden)

    Omar Huertas Díaz

    2009-06-01

    Full Text Available En el mundo de hoy, el desarrollo económico y social se gestiona en términos de investigación, por cuanto ésta proporciona elementos fundamentales para aprovechar los recursos disponibles, estimular el desarrollo de talentos, ofrecer igualdad de oportunidades, darles valor agregado y, articular la economíadentro de los procesos del mercado global y la competitividad.

  9. Artes y posconflicto

    Directory of Open Access Journals (Sweden)

    PENSAMIENTO, PALABRA Y OBRA

    2014-12-01

    No bastará que las artes estén presentes en el nuevo escenario social. Tenemos que formular política y tenemos que formar  a  quienes  se  encargarán  de darle sentido a las artes en la escuela, en los espacios comunitarios, en los medios masivos, en fin, en la vida.

  10. La sentencia C-577 de 2011 y el matrimonio igualitario en Colombia

    Directory of Open Access Journals (Sweden)

    Manuel Páez Ramírez

    2013-12-01

    Full Text Available El presente artículo ofrece razones jurídicas para considerar que la forma más razonable y constitucional de darle cumplimiento a la sentencia C-577 de 2011 de la Corte Constitucional en lo referente a la formalización y solemnización del vínculo marital entre parejas del mismo sexo, es mediante el contrato de matrimonio civil.

  11. La sentencia C-577 de 2011 y el matrimonio igualitario en Colombia

    OpenAIRE

    Manuel Páez Ramírez

    2014-01-01

    El presente artículo ofrece razones jurídicas para considerar que la forma más razonable y constitucional de darle cumplimiento a la sentencia C-577 de 2011 de la Corte Constitucional en lo referente a la formalización y solemnización del vínculo marital entre parejas del mismo sexo, es mediante el contrato de matrimonio civil.

  12. La sentencia C-577 de 2011 y el matrimonio igualitario en Colombia

    Directory of Open Access Journals (Sweden)

    Manuel Páez Ramírez

    2014-01-01

    Full Text Available El presente artículo ofrece razones jurídicas para considerar que la forma más razonable y constitucional de darle cumplimiento a la sentencia C-577 de 2011 de la Corte Constitucional en lo referente a la formalización y solemnización del vínculo marital entre parejas del mismo sexo, es mediante el contrato de matrimonio civil.

  13. Realitas Citra Politik Tri Rismaharini

    OpenAIRE

    Ali Sahab

    2017-01-01

    Tri Rismaharini (Risma) is the Mayor of Surabaya who had successfully led Surabaya during the period of 2010-2015. In 2015 mayoral election, Risma was re-elected as the Mayor of Surabaya. Risma has been known as a mayor who really concerns about environment. During her first tenure, Risma had built many city parks, successfully improved beauty of Surabaya. Such leadership style had attracted the public. In addition, Risma had become a media darling in both printed and electronic media, locall...

  14. Mobilization of the south against food crisis

    Directory of Open Access Journals (Sweden)

    Laurent Delcourt

    2009-06-01

    Full Text Available “Creemos que a esta crisis alimentaria hay que darle un nombre más exacto. Todavía no damos con ese nombre. Pero no es una crisis de alimentos,es una crisis de valores en las empresas transnacionales, en los gobiernos y en la cooperación internacional”, Sinforiano Cáceres, presidente de la Federación nacional de cooperativas, Nicaragua (Envío, 2008.

  15. A new species of Smicromorpha (Hymenoptera, Chalcididae from Vietnam, with notes on the host association of the genus

    Directory of Open Access Journals (Sweden)

    Chris Darling

    2009-09-01

    Full Text Available Smicromorpha masneri Darling, sp. n. is described from Bach Ma National Park, Thua Thien-Hue Province, Vietnam. This is the first confirmed record for the genus on mainland southeast Asia. It is generally accepted that species of Smicromorpha are parasitoids of green tree or weaver ants, Oecophylla smaragdina (Fabricius but this is first rearing record of these parasitoids from the nests of weaver ants.

  16. SRM, motor para el desarrollo de la cadena de valor Supplier Relationship Management – SRM gestión de las relaciones con los proveedores

    Directory of Open Access Journals (Sweden)

    Luis Leonardo Mendoza S.

    2010-01-01

    Ejemplo de ello es el SRM, Supplier Relationship Management, en donde por medio de la constitución de acuerdos basados en intereses comunes, se establecen relaciones estrechas, funcionales y eficientes entre las partes, permitiendo por esta vía la garantía del suministro, y de esta forma, darle un importante impulso para el desarrollo de la cadena de valor.

  17. A nonpedagogical teacher education?

    DEFF Research Database (Denmark)

    Kvols, Anja Madsen; Madsen, Peter Hougaard; Beck, Mette Holdsendorf

    2017-01-01

    regarding changes in education – which seem to come from evaluations more than from educational and pedagogical theories (Gruschka, 2011). Moreover, the teacher education has increased its focus on both research-based academic training and professional training at the same time. Our study is double – 1...... characterizes teacher professionalism? (Subject studies? Pedagogical studies? Professional training? Academic studies? Bildung studies? Etc.) - What characterizes ‘pedagogy of teacher education’ (Darling-Hammond, 2006; Loughran, 2008; Zeichner, 2005)?...

  18. A Review of Models of the Human Temperature Regulation System.

    Science.gov (United States)

    1992-02-18

    numerous experimental data for, the forearm, but it was essentially applicable to any cylindrical representation of an element of the body. Because the...Herzfeld, (Ed.), Reinhold, New York, NY, pp. 637-665, 1963. 11. Downey, J.A., Phil, D., and Darling, R.C., " Thermotherapy and Thermoregulation...Burton, A.C., "The Application of the Theory of Heat Flow to the Study of Energy Metabolism." Journal of Nutrition, Vol. 7, pp. 497-533, 1934. 119

  19. Stresses and Strains in a Cold-Worked Annulus

    Science.gov (United States)

    1988-09-01

    Airways Limited Ansett Airlines of Australia, Library BHP, Melbourne Research Laboratories Hawker de Havilland Aust Pty Ltd, Victoria, Library Hawker de...Library Statutory and State Authorities and Industry Aero-Space Technologies Australia, Manager/Librarian (2 copies) Australian Airlines, Library Qantas ...DOWNGRADING DARL APPROVAL 12. SECDARY DISTRIBUTION (OF THIS DoCUmr) Approved for public release. OVERSEAS ?2KQUIRIES OUTSIDE STATED LIMITATIONS SHOULD BE

  20. DETECCION DE CARIES DENTAL CON EL SISTEMA ICDAS II Y EL ÍNDICE CPO-S, EN NIÑOS DE 6 A 8 AÑOS ATENDIDOS EN EL CENTRO COMUNITARIO DE LA UNIVERSIDAD CATOLICA SANTA MARIA CIUDAD DE DIOS-YURA. AREQUIPA, 2016

    OpenAIRE

    ÁLVAREZ COAILA, KAREN EVELYN

    2016-01-01

    CARIES DENTAL EN ESMALTE ZONA SUPERFICIAL O CAPA DE DARLING CUERPO DE LA LESIÓN O ZONA SUB-SUPERFICIAL ZONA OSCURA ZONA SUB TRANSPARENTE EN DENTINA DENTINA INFECTADA DENTINA AFECTADA ÍNDICES EPIDEMIOLÓGICOS TIPOS DE ÍNDICES DE CARIES DESVENTAJAS DE LA DETECCIÓN DE CARIES DENTAL CRITERIOS OMS2 SISTEMA DE DETECCIÓN Y VALORACIÓN DE CARIES DENTAL (ICDAS) ANTECEDENTES INVESTIGATIVOS RESULTADOS

  1. Implicaciones del gobierno corporativo en las economías emergentes

    Directory of Open Access Journals (Sweden)

    Lorena Garnica de la Espriella

    2009-12-01

    Full Text Available El presente artículo tiene como objetivo exponer el comportamiento delos mercados latinoamericanos, los cuales, como economías emergentes, deben darle prioridad al concepto de buen gobierno corporativo para propender por el crecimiento de sus empresas y evitar las crisis societarias. Se explicarán las actuaciones acertadas al interior de las sociedades, para conseguir una satisfactoria incidencia en el mercado.

  2. Genome-Wide Association Study Provides Insight into the Genetic Control of Plant Height in Rapeseed (Brassica napus L.)

    OpenAIRE

    Chengming Sun; Benqi Wang; Lei Yan; Kaining Hu; Sheng Liu; Yongming Zhou; Chunyun Guan; Zhenqian Zhang; Jiana Li; Jiefu Zhang; Song Chen; Jing Wen; Chaozhi Ma; Jinxing Tu; Jinxiong Shen

    2016-01-01

    Plant height is a key morphological trait of rapeseed. In this study, we measured plant height of a rapeseed population across six environments. This population contains 476 inbred lines representing the major Chinese rapeseed genepool and 44 lines from other countries. The 60K Brassica Infinium® SNP array was utilized to genotype the association panel. A genome-wide association study (GWAS) was performed via three methods, including a robust, novel, nonparametric Anderson-Darling (A-D) test....

  3. Performance of Jatropha curcas L. in Semi-arid Zone: Seed Germination, Seedling Growth and Early Field Growth

    Directory of Open Access Journals (Sweden)

    Sharif AHAMAD

    2013-05-01

    Full Text Available There is a lack of information on basic agronomic properties of Jatropha curcas L. (jatropha cultivation on the marginal lands in the semi-arids. Evaluation of agronomic performance of identified elite strains of J. curcas in marginal lands would be of paramount importance for addressing gap areas in their agronomic properties and subsequently for harnessing their optimum economic potentials. The present study undertook the task of analysing the growth performance of a high oil bearing elite strain of J. curcas–DARL-2 in degraded land in semi-arid zone of Deccan Plateau, India. While undertaking the assessment of growth performance of elite strain DARL-2, two other native (wild strains (namely AHN-1 and AHN-2 of J. curcas were also considered so that a comparative evaluation could be carried out. The role of gypsum was also investigated on J. curcas in the nursery stage as well its carry over effects on growth performance of transplanted trees in the field. Two types of substrates, gypsum-treated soil (GS and untreated soil (SL were used for growing seedlings of all the three jatropha strains. Seedlings (120-days-old of DARL-2 exhibited greater plant height, collar diameter and number of branches but root length was greater in the local strains. In the second year of field transplantation, DARL-2 strain exhibited significantly (p<0.05 greater plant height and number of branches/plant. No carry over effects of gypsum treatment were observed in field transplanted plants as none of the growth parameters significantly varied among the substrate types.

  4. Goodness-of-fit tests for the Gompertz distribution

    DEFF Research Database (Denmark)

    Lenart, Adam; Missov, Trifon I.

    2016-01-01

    While the Gompertz distribution is often fitted to lifespan data, testing whether the fit satisfies theoretical criteria is being neglected. Here four goodness-of-fit measures- the Anderson-Darling statistic, the correlation coefficient test, a statistic using moments and a nested test against...... the generalized extreme value distributions, are discussed. Along with an application to laboratory rat data, critical values calculated by the empirical distribution of the test statistics are also presented....

  5. Goodness-of-fit tests for the Gompertz distribution

    DEFF Research Database (Denmark)

    Lenart, Adam; Missov, Trifon

    The Gompertz distribution is often fitted to lifespan data, however testing whether the fit satisfies theoretical criteria was neglected. Here five goodness-of-fit measures, the Anderson-Darling statistic, the Kullback-Leibler discrimination information, the correlation coefficient test, testing...... for the mean of the sample hazard and a nested test against the generalized extreme value distributions are discussed. Along with an application to laboratory rat data, critical values calculated by the empirical distribution of the test statistics are also presented....

  6. Ehtekunsti kõrgmäestik Münchenis / Kadri Mälk, Piret Hirv, Tanel Veenre

    Index Scriptorium Estoniae

    Mälk, Kadri, 1958-

    2009-01-01

    Ehtekunsti suurüritusest "Schmuckszene" ("Ehtepaik"). Arnoldsche Art Publishers esitles viit aasta paremaks ehtekunstiraamatuks tituleeritut, nende seas Eestis trükikojas Pakett trükitud raamatut "Just must" (kujundaja Aadam Kaarma, fotod: Tanel Veenre). Darling Publications'i poolt esitletud raamatust "The Compendium Finale of Contemporary Jewellery Makes", kus tutvustatakse kümmet eesti ehtekunstnikku, tekstide autorid on Kadri Mälk, Harry Liivrand ja Tamara Luuk

  7. Realidad virtual como buena práctica para trabajo en equipo con estudiantes de ingeniería

    OpenAIRE

    Gasca-Hurtado, Gloria Piedad; Peña Pérez Negrón, Adriana; Gómez-Álvarez, María Clara; Plascencia-Osuna, Óscar Armando; Calvo-Manzano Villalón, José Antonio

    2015-01-01

    La realidad virtual es una alternativa de motivación de los estudiantes al darles la posibilidad de realizar acciones como volar u observar el entorno desde diferentes perspectivas de manera controlada. La interacción multiusuario en realidad virtual da lugar a los entornos virtuales colaborativos que favorecen el desarrollo de habilidades sociales como el trabajo en equipo, en particular en equipos con integrantes con ubicaciones geográficas diferentes. En estos contextos se encuentran diver...

  8. Diversity in Health: Sharing Global Perspectives. 28–30 May 2001, Sydney, Australia

    OpenAIRE

    Bandawe, C

    2001-01-01

    This landmark conference on multicultural health and well being was held in the Sydney Convention ind Exhibition Centre overlooking Darling Harbour. The conference was organised by the Australian Transcultural Mental Health Network as well as the Australian Multicultural Health Conference. It brought together over 300 delegates mainly from Australia. A unique feature of this conference was the presentation of five speakers from Southern Africa who gave an African perspective to health and wel...

  9. Bringing ecosystem services into integrated water resources management.

    Science.gov (United States)

    Liu, Shuang; Crossman, Neville D; Nolan, Martin; Ghirmay, Hiyoba

    2013-11-15

    In this paper we propose an ecosystem service framework to support integrated water resource management and apply it to the Murray-Darling Basin in Australia. Water resources in the Murray-Darling Basin have been over-allocated for irrigation use with the consequent degradation of freshwater ecosystems. In line with integrated water resource management principles, Australian Government reforms are reducing the amount of water diverted for irrigation to improve ecosystem health. However, limited understanding of the broader benefits and trade-offs associated with reducing irrigation diversions has hampered the planning process supporting this reform. Ecosystem services offer an integrative framework to identify the broader benefits associated with integrated water resource management in the Murray-Darling Basin, thereby providing support for the Government to reform decision-making. We conducted a multi-criteria decision analysis for ranking regional potentials to provide ecosystem services at river basin scale. We surveyed the wider public about their understanding of, and priorities for, managing ecosystem services and then integrated the results with spatially explicit indicators of ecosystem service provision. The preliminary results of this work identified the sub-catchments with the greatest potential synergies and trade-offs of ecosystem service provision under the integrated water resources management reform process. With future development, our framework could be used as a decision support tool by those grappling with the challenge of the sustainable allocation of water between irrigation and the environment. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

  10. Selling “Performance” Assessments with Inaccurate Pictures from Kentucky

    Directory of Open Access Journals (Sweden)

    Richard G. Innes

    2015-02-01

    Full Text Available A new white paper from Ace Parsi and Linda Darling-Hammond, “Performance Assessments: How State Policy Can Advance Assessments for 21st Century Learning,” has a discussion on Page 7 about supposed success of the Kentucky Instructional Results Information System (KIRIS used in that state from 1992 to 1998. This discussion paper explains the numerous problems with that description. The Kentucky-related comments in the Parsi/Darling-Hammond paper claim a basis in a technical report titled “Commonwealth Accountability and Testing System: 2007–08 Technical report, Version 1.2” created by Measured Progress in 2009. Unfortunately, the Kentucky Department of Education recently redesigned its web site and this technical report is no longer online. However, if the report actually does show the information attributed to it by Parsi and Darling-Hammond, that information is incorrect. Of course, as a closeout epitaph of yet another failure, Kentucky’s now failed Commonwealth Accountability Testing System (CATS, which succeeded the failed KIRIS system, the 2007-08 technical report may not have received an appropriate level of attention to detail. The comments below are referenced to reports issued during or shortly after the events occurred and are known to this long-time Kentucky resident to present an accurate picture.

  11. Histoplasmosis in patients with human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS): multicenter study of outcomes and factors associated with relapse.

    Science.gov (United States)

    Myint, Thein; Anderson, Albert M; Sanchez, Alejandro; Farabi, Alireza; Hage, Chadi; Baddley, John W; Jhaveri, Malhar; Greenberg, Richard N; Bamberger, David M; Rodgers, Mark; Crawford, Timothy N; Wheat, L Joseph

    2014-01-01

    Although discontinuation of suppressive antifungal therapy for acquired immunodeficiency syndrome (AIDS)-associated histoplasmosis is accepted for patients with immunologic recovery, there have been no published studies of this approach in clinical practice, and minimal characterization of individuals who relapse with this disease. We performed a multicenter retrospective cohort study to determine the outcome in AIDS patients following discontinuation of suppressive antifungal therapy for histoplasmosis. Ninety-seven patients were divided into a physician-discontinued suppressive therapy group (PD) (38 patients) and a physician-continued suppressive therapy group (PC) (59 patients). The 2 groups were not statistically different at baseline, but at discontinuation of therapy and at the most recent follow-up there were significant differences in adherence to therapy, human immunodeficiency virus (HIV) RNA, and urinary Histoplasma antigen concentration. There was no relapse or death attributed to histoplasmosis in the PD group compared with 36% relapse (p 150 cells/mL, HIV RNA <400 c/mL, Histoplasma antigenuria <2 ng/mL (equivalent to <4.0 units in second-generation method), and no CNS histoplasmosis.

  12. Histoplasmosis in Patients With Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS)

    Science.gov (United States)

    Anderson, Albert M.; Sanchez, Alejandro; Farabi, Alireza; Hage, Chadi; Baddley, John W.; Jhaveri, Malhar; Greenberg, Richard N.; Bamberger, David M.; Rodgers, Mark; Crawford, Timothy N.; Wheat, L. Joseph

    2014-01-01

    Abstract Although discontinuation of suppressive antifungal therapy for acquired immunodeficiency syndrome (AIDS)-associated histoplasmosis is accepted for patients with immunologic recovery, there have been no published studies of this approach in clinical practice, and minimal characterization of individuals who relapse with this disease. We performed a multicenter retrospective cohort study to determine the outcome in AIDS patients following discontinuation of suppressive antifungal therapy for histoplasmosis. Ninety-seven patients were divided into a physician-discontinued suppressive therapy group (PD) (38 patients) and a physician-continued suppressive therapy group (PC) (59 patients). The 2 groups were not statistically different at baseline, but at discontinuation of therapy and at the most recent follow-up there were significant differences in adherence to therapy, human immunodeficiency virus (HIV) RNA, and urinary Histoplasma antigen concentration. There was no relapse or death attributed to histoplasmosis in the PD group compared with 36% relapse (p 150 cells/mL, HIV RNA <400 c/mL, Histoplasma antigenuria <2 ng/mL (equivalent to <4.0 units in second-generation method), and no CNS histoplasmosis. PMID:24378739

  13. Stretch-bend combination polyads in the à 1A u state of acetylene, C 2H 2

    Science.gov (United States)

    Steeves, Adam H.; Bechtel, Hans A.; Merer, Anthony J.; Yamakita, Nami; Tsuchiya, Soji; Field, Robert W.

    2009-08-01

    Rotational analyses are reported for a number of newly-discovered vibrational levels of the S 1- trans (Ã 1A u) state of C 2H 2. These levels are combinations where the Franck-Condon active ν2' and ν3' vibrational modes are excited together with the low-lying bending vibrations, ν4' and ν6'. The structures of the bands are complicated by strong a- and b-axis Coriolis coupling, as well as Darling-Dennison resonance for those bands that involve overtones of the bending vibrations. The most interesting result is the strong anharmonicity in the combinations of ν3' ( trans bend, a g) and ν6' (in-plane cis bend, b u). This anharmonicity presumably represents the approach of the molecule to the trans- cis isomerization barrier, where ab initio results have predicted the transition state to be half-linear, corresponding to simultaneous excitation of ν3' and ν6'. The anharmonicity also causes difficulty in the least squares fitting of some of the polyads, because the simple model of Coriolis coupling and Darling-Dennison resonance starts to break down. The effective Darling-Dennison parameter, K4466, is found to increase rapidly with excitation of ν3', while many small centrifugal distortion terms have had to be included in the least squares fits in order to reproduce the rotational structure correctly. Fermi resonances become important where the K-structures of different polyads overlap, as happens with the 2 13 1B1 and 3 1B3 polyads ( B = 4 or 6). The aim of this work is to establish the detailed vibrational level structure of the S 1- trans state in order to search for possible S 1- cis ( 1A 2) levels. This work, along with results from other workers, identifies at least one K sub-level of every single vibrational level expected up to a vibrational energy of 3500 cm -1.

  14. A rapid and simple method for identifying bacterial polar lipid components in wet biomass.

    Science.gov (United States)

    Nguyen, Tuan Manh; Kim, Jaisoo

    2017-08-01

    There are marked differences between wet and freeze-dried cells with regard to the identification of polar lipid components. The determination of the polar lipid composition of freeze-dried cells is well established. However, several approaches to identifying polar lipid components in wet cells have met with limited success owing to the presence of non-polar compounds in the extracts, resulting in a lipid composition with a narrow scope. In this study, we surveyed the lipid profiles of the wet biomasses of three Gram-positive (Microbacterium lacticum, Rhodococcus koreensis, and Streptomyces longwoodensis) and two Gram-negative (Pseudomonas aeruginosa and Novosphingobium capsulatum) bacteria; the results were comparable in quality to those obtained using a standard freeze-dried approach. Moreover, our improved method ensures simple lipid extraction. Overall, the results of the analysis showed minor lipid profile differences between the two approaches with regard to quantity, and lipid identification was consistent in both methods for all species.

  15. Reações à histoplasmina e paracoccidioidina na Serra de Pereiro (estado do Ceará-Brasil Hypersensitivity to histoplasmin and paracoccidioidin in people living in Pereiro, Ceará, Brazil

    Directory of Open Access Journals (Sweden)

    M. J. N. Diógenes

    1990-04-01

    Full Text Available Foram realizados 138 testes com histoplasmina e paracoccidioidina no Município de Pereiro - Ceará, obtendo-se positividade de 61,5% e 32,1% respectivamente, a estes antígenos. Os autores discutem a possibilidade da coexistência da histoplasmose e paracoccidioidomicose-infecção e/ou doença na região.Intradermal tests using histoplasmin and paracoccidioidin antigens were performed in 138 persons from Pereiro, Ceará, Brazil. The results were positive in 61.5% and 32.5% with histoplasmin and paracoccidioidin antigens respectively. These results suggest infection by H. capsulatum and P. brasiliensis in the people living in the studied area. New studies are necessary to detect histoplasmosis and paracoccidioidomycosis as clinical diseases in that region.

  16. Amenazas actuales a la institucionalidad electoral en América Latina: La experiencia panameña

    Directory of Open Access Journals (Sweden)

    Eduardo Valdés Escoffery

    2017-07-01

    Full Text Available Analiza la reforma constitucional de Panamá de 2004 y el conflicto de competencias que genera al darle a la Corte Suprema de Justica la facultad exclusiva para juzgar penalmente a los diputados, sin excepción por tipo de delito. Por tal motivo, juzgará también los delitos electorales y por ende de forma implícita tendrá la potestad de interpretar la normativa electoral; competencia privativa del Tribunal Electoral desde su creación en el año 1956, donde se le asigna la jurisdicción de interpretar, reglamentar y aplicar la ley electoral.

  17. Los sistemas de gestión de contenidos como generadores de sinergias y redes de colaboración: relato de dos experiencias peruanas

    OpenAIRE

    Arias Martínez, Luis Miguel; Vílchez Román, Carlos

    2009-01-01

    Ponencias de la Segunda Conferencia Internacional sobre brecha digital e inclusión social, celebrada del 28 al 30 de octubre de 2009 en la Universidad Carlos III de Madrid En marzo de 2005, una propuesta de articulación de colaboración entre universidades públicas en Perú, puso a nuestra disposición un sistema de gestión de contenidos útil para darle soporte a la coordinación de la Red de Universidades Públicas para la Autoevaluación y Mejora Continua (REDUNIV´s). El proceso de apropiación...

  18. La educación y la voluntad en kant

    OpenAIRE

    Vásquez Mendoza, Evelyn; Caro, Clara Virginia

    2010-01-01

    Es un hecho bastante bien reconocido que los grandes hombres del mundo siempre han perseguido un gran ideal: hacer el mayor bien a la humanidad, buscar al hombre para hacerle el don de si-mismo y de todo su potencial humano, intelectual y moral. Uno de esos grandes hombres que siempre se preocupó por las otras existencias fue Emmanuel Kant. Este pensador halló su realización en el trabajo continuo por la promoción del hombre, no ahorró esfuerzo alguno para darle al hombre luces sobre...

  19. La dimensión política en la constitución de la identidad del sujeto.

    OpenAIRE

    Rafael Sandoval Álvarez

    2000-01-01

    En este ensayo se pretende dar un acercamiento a los elementos que constituyen la dimensión política de la identidad del sujeto. Eventualmente se utilizará, como ejemplo, un sujeto concreto: algunos actores de la sociedad civil que se han destacado por darle una resignificación y redimensionamiento a lo político, específicamente en dos sentidos: uno sobre las formas de hacer y pensar la política y, otro, sobre la recuperación de los espacios de lo público q...

  20. La dimensión política en la constitución de la identidad del sujeto.

    Directory of Open Access Journals (Sweden)

    Rafael Sandoval Álvarez

    2000-01-01

    Full Text Available En este ensayo se pretende dar un acercamiento a los elementos que constituyen la dimensión política de la identidad del sujeto. Eventualmente se utilizará, como ejemplo, un sujeto concreto: algunos actores de la sociedad civil que se han destacado por darle una resignificación y redimensionamiento a lo político, específicamente en dos sentidos: uno sobre las formas de hacer y pensar la política y, otro, sobre la recuperación de los espacios de lo público que estaban ocupados exclusivamente por los partidos y el gobierno.

  1. Redes deseantes. Tendencias político-emocionales en redes sociales

    OpenAIRE

    Torres Nabel, Luis César

    2016-01-01

    El artículo expone el concepto de red deseante para explicar  los fenómenos virales que suceden en las redes sociales. Las redes  deseantes constituyen una ruta alterna a la necesidad evidente y frustrada por la contexto, principalmente si se trata de un contexto anómico. Para darle sustento y robustez al concepto se desarrolla un análisis a partir del primer estudio basado en la metodología del big data en México donde el Instituto Nacional de Estadística y Geografía expuso el estado de ánim...

  2. Propuesta comunicativa para la creación del departamento de comunicación en Diners Club del Ecuador en la sucursal de Quito.

    OpenAIRE

    Arroyo Vanegas, Cynthia Berenice

    2011-01-01

    La comunicación hoy en día, es primordial e importante para el desarrollo de toda índole, pasando a ser más que un simple acto comunicativo, por lo cual es necesario darle importancia en el ámbito organizacional. Esta nueva comunicación innovadora parte del nuevo paradigma, permitiendo alcanzar de esta manera una comunicación organizacional más: dinámica, organizada, evolutiva, recreadora, abierta, flexible y participativa. La comunicación dentro de una empresa se encuentra presente en toda a...

  3. LIDERAZGO Y MOTIVACIÓN EN EL AMBIENTE EDUCATIVO UNIVERSITARIO (LEADERSHIP AND MOTIVATION IN THE UNIVERSITY EDUCATIONAL ENVIRONMENT)

    OpenAIRE

    Alvarado Yajaira; Prieto Sánchez Ana Teresa; Betancourt Daryeling

    2009-01-01

    Resumen:Las tareas del líder con respecto a la motivación son muchas y variadas. Entre otras cosas, un líder debe reconocer la necesidad de sus seguidores, ayudarlos a ver como pueden concretar esas necesidades y darles la confianza para que puedan alcanzar a remover constricciones o inhibiciones que han impedido la motivación. Esta inquietud ha sido la impulsora del presente artículo, que tiene como objetivo analizar el liderazgo y la motivación en el ambiente educativo universitario, concib...

  4. LA ORALIDAD COMO PARTE DE LA INVESTIGACIÓN HISTÓRICA: DE LAS FUENTES ORALES A LA LLAMADA HISTORIA ORAL

    Directory of Open Access Journals (Sweden)

    José Ignacio Reyes González

    2010-03-01

    Full Text Available El artículo profundiza en uno de los temas más polémicos de la investigación histórica, lo relacionado con el reconocimiento del valor de las fuentes orales para reconstruir la historia. Se exponen los argumentos que fundamentan la necesidad de darle el espacio que merece la historia oral, así como la connotación que esto puede tener para la enseñanza de la disciplina.

  5. La deculturación de identidad en El entierro de Cortijo de Edgardo Rodríguez Juliá.

    OpenAIRE

    Badillo, William

    2012-01-01

    Este ensayo presenta la vida de un colectivo social que por más de cuatrocientos años ha sido víctima del aislamiento cultural. En pleno desarrollo manufacturero a mediados del siglo veinte en la isla, la raza negra no tuvo las mismas oportunidades de empleo en el área urbana de la ciudad. El autor hace uso de la crónica para darle voz a una clase social desamparada que va camino a un entierro. Este artículo refleja la desigualdad y la segregación cultural junto con los efectos negativos que ...

  6. Casos prácticos Normativa APA 6ª ed.

    OpenAIRE

    Guerras-Frías, Miroslava (tr)

    2010-01-01

    A la hora de realizar un trabajo es importante leer otros documentos relacionados para ver el estado de la cuestión, además de apoyarte en sus teorías para darle valor y peso a tu trabajo. Pero todo trabajo que este basado en otros debe de citar y tener sus referencias bibliográficas normalizadas. Para esto hay muchos estilos validos normalizados, el que aquí nos ocupa es sobre el estilo de la American Psychological Association, más conocido como Nomas o Estilo APA de la 6ª edición.

  7. Pneumatización del seno maxilar. Una propuesta de clasificación.

    OpenAIRE

    Sager Ramseyer, Fernando Oscar

    2016-01-01

    En los últimos 40 años la odontología ha experimentado un avance sin precedentes que podemos observar en el desarrollo de nuevos materiales, evolución de técnicas y métodos, aparición de elementos diagnósticos más precisos o nuevas alternativas de tratamiento para aquellas personas a quienes las opciones terapéuticas tradicionales no puedan darle solución, ya sea por las limitaciones de éstas o por las exigencias propias del paciente. Paralelamente al avance de la odontolog...

  8. Metodología para el análisis de información en lavado de activos y financiación del terrorismo de proveedores, clientes e inversionistas en empresas colombianas de importación y exportación

    OpenAIRE

    Acuña Ríos, Heriberto

    2011-01-01

    RESUMEN: El lavado de activos comprende todas aquellas actividades ejecutadas por personas naturales o jurídicas encaminadas a darle apariencia de legalidad a bienes de origen ilícito. Y la financiación del terrorismo está constituida por la financiación de actividades ilícitas ejecutadas por organizaciones terroristas. En el marco de la Ley 1151 de 2007, se establece la política contra el lavado de activos y la financiación del terrorismo enmarcada en la lucha contra los grupos armados...

  9. El encuentro entre arte y militancia y la publicidad de ideas políticas en el espacio público callejero : El ejemplo del GAC

    OpenAIRE

    Russo, Pablo M.

    2008-01-01

    Se ha vuelto común hablar de la muerte del espacio público y la crisis de las formas tradicionales de la representación política. Frente a este fenómeno, el espacio público callejero invita a una renovación en las discusiones y los debates para aquellos que buscan darle publicidad a sus ideas políticas. En las ciudades, esto se vuelve aun más evidente en momentos de mayor movilización social, cuando grupos artísticos se expresan políticamente, y colectivos sociales buscan una veta e...

  10. Trastorno borderline : un viaje hacia el centro de los límites

    OpenAIRE

    Salcedo Pérez, Raquel

    2014-01-01

    Lo límite, borderline o limítrofe ha suscitado desde sus orígenes gran interés y asombro a los autores que trataban de darle forma. Se revisan aspectos generales del concepto hasta situarse en lo límite como organización estructural de la personalidad de manos del Psicoanálisis. Se utiliza para este acercamiento la terapia de expresión de Kernberg para entender por medio de la Transferencia algunos aspectos intrapsíquicos de la personalidad de estos sujetos

  11. Usted puede ser Empresario!

    Directory of Open Access Journals (Sweden)

    Pedro A. López Guzmán

    1988-04-01

    Full Text Available RESUMEN Con este artículo se pretende  que usted  señor lectos ea  un empresario consciente y lo más competente posible. A medida que usted mismo pueda plantearse un problema e ir  resumiendo y analizando los datos que ha obtenido, verá de qué manera esa práctica contribuirá a darle la claridad mental necesaria para encarar con éxito su tarea de Empresario.

  12. A mi pintura le gustaría llegar a la textura de la piedra, de un árbol o del agua

    Directory of Open Access Journals (Sweden)

    Kindi Llajtu

    2014-09-01

    Full Text Available Kindi Llajtu viene trabajando con las huellas que encuentra y las que él mismo produce y deja atrás por medio de intervenciones en el espacio, los cuerpos y las superficies. Recuperar los rastros del pueblo Inga al que pertenece, sus antiguos objetos, sus comidas, sus fiestas, para darles nueva vivacidad o hacerlos desvanecer de una nueva manera, tiene por finalidad generar y jugar con las texturas del recuerdo que cubre y descubre todo; hacer texturas que evidencian el paso del tiempo y sus fantasmas que lo van puliendo, tejiendo y transformando todo.

  13. E l coaching ontológico como estrategia para gerenciar el aprendizaje, gestionar el conocimiento, transformar los procesos educativos y potenciar cambios significativos / Ontological coaching as a strategy for learning manage, manage knowledge, transforming processes promote education and significant changes

    OpenAIRE

    Ortega Guizado, Remberto

    2012-01-01

    El mundo está cambiando a tal velocidad que todos los modelos tradicionales, incluyendo el educativo, se extinguen delante de nuestros ojos. Es por esto que, hoy más que nunca, el crecimiento y la rentabilidad de la propuesta educativa depende en gran medida de que sus líderes sean capaces de darle poder y dignidad a la persona y hacerla responsable de su manera de ser, hacer y de los resultados que generan sus esfuerzos. Pero para ello es necesario que el modelo educativo enseñe a aprender a...

  14. Sistema de actividades para lograr que la Secundaria Básica se convierta en Palacio de Pioneros

    Directory of Open Access Journals (Sweden)

    Margot Hernández Leyva

    2017-11-01

    Full Text Available Se propone un sistema de acciones para darle salida al funcionamiento de la escuela como palacio de pioneros, teniendo en cuenta la integración de componentes como: la educación patriótica, la orientación profesional, la educación física y el deporte, la educación artística y estética, La salud escolar y el trabajo preventivo, y la educación laboral.

  15. El capital social y su tratamiento en las entidades en las entidades de ahorro y crédito popular sobre la base de la normativa contable internacional

    Directory of Open Access Journals (Sweden)

    Luis Carlos Gámez Adame

    2013-03-01

    Full Text Available Las Entidades de Ahorro y Crédito Popular (Cooperativas constituyen un segmento importante dentro de los servicios financieros que se prestan en diferentes países y que han tenido un desarrollo vertiginoso al satisfacer demandas y necesidades de numerosas masas poblacionales.Se presentan algunas consideraciones asociadas al tratamiento del capital social en estas entidades sobre la base de la normatividad contable internacional vigente y donde a criterio de los autores existen una serie de incongruencias a las que hay que darles solución.

  16. Liderazgo y autoeficacia en el comportamiento organizacional positivo

    OpenAIRE

    Sanchez Hernandez, Maria Paula; Pinto Erazo, Laura María; Contreras Ruiz, Ivan Camilo

    2016-01-01

    El objetivo que tiene este proyecto es revisar los conceptos básicos acerca de las relaciones que crean los líderes con sus colaboradores dentro de las organizaciones, dichas relaciones y vínculos pueden afectar positiva o negativamente el desempeño de sus actividades diarias dentro de una organización. Para darle inicio a la investigación se estudió como primer paso el concepto de liderazgo transformacional, capital psicológico y que componentes hacían parte de este factor. El desarrollo ...

  17. Teleportation the impossible leap

    CERN Document Server

    Darling, David

    2005-01-01

    An authoritative, entertaining examination of the ultimate thrill ride Until recently the stuff of sci-fi fiction and Star Trek reruns, teleportation has become a reality-for subatomic particles at least. In this eye-opening book, science author David Darling follows the remarkable evolution of teleportation, visiting the key labs that have cradled this cutting-edge science and relating the all-too-human stories behind its birth. He ties in the fast emerging fields of cryptography and quantum computing, tackles some thorny philosophical questions (for instance, can a soul be teleported?)

  18. Hacia la optimización del proceso de desarrollo humano y social en su aspecto creativo e innovador, vinculado al emprendimiento y al espíritu empresarial constructivo

    OpenAIRE

    Calleja Montani, Walter Hugo

    2014-01-01

    El presente trabajo de Investigación tiene el enfoque de promover e impulsar una nueva generación de líderes empresariales bien motivados tengan inteligencia espiritual, tengan inteligencia emocional, tengan cuerpo y corazones sanos; así como también eficientes, efectivos y eficaces . Darle oportunidad al desarrollo de empresarios jóvenes con capacidad de emprendedores que sean destacados en el sistema social y económico como líderes de base, creativos e innovadores, amantes de propiciar los ...

  19. para aspirar al éxito de la empresa

    Directory of Open Access Journals (Sweden)

    Orlando Vásquez Castro

    2002-01-01

    Full Text Available El pensamiento estratégico tiene que traducirse a implementación estratégica para alcanzar las metas por las que lucha la organización. De no ser así, todo el proceso de planeación, tan claro, ordenado y controlado, falla en alcanzar los resultados y termina como letra muerta. El propósito del artículo es darles guías, caminos y herramientas a aquellos que quieren aceptar el desafío de la implementación estratégica en sus empresas con mínima ayuda externa.

  20. Merging hydraulics with biology

    Energy Technology Data Exchange (ETDEWEB)

    Thorncraft, Garry; Baumgartner, Lee J.; Boys, Craig A.; Brown, Richard S.

    2013-01-01

    Research staff are now working with developers in Lao PDR and Australia to ensure design criteria is optimised at time of construction. Research is presently underway on a suite of Mekong and Murray-Darling species to identify critical tolerances of shear and pressure. Work in the USA is also being expanded to lamprey and white sturgeon in an effort to increase the understanding beyond salmon species. Researchers and developers are hopeful of constructing the first fish-friendly hydro plants within the next two years. Pending favourable outcomes, the expansion of this technology to other sites and river systems is the ultimate development outcome.

  1. Proceso de construcción de la identidad del adolescente que consulta en la Comisaría de Familia de la ciudad de Manizales

    OpenAIRE

    Pinilla Amador, Claudia Patricia

    2000-01-01

    Tesis de grado (Maestría en Pedagogías Activas y Desarrollo Humano). Centro Internacional de Educación y Desarrollo humano -Cinde-Universidad de Manizales. Facultad de Ciencias Sociales y Humanas, 2000 La investigación desarrollada se implementa dentro de la perspectiva Histórico Hermenéutica, busca darle un sentido a la información obtenida a través de instrumentos como una guía de registro y análisis, la entrevista, la historia de vida y la prueba de autoconcepto de Tennessee; utiliza...

  2. LA POESÍA EN EL AULA: UNA PROPUESTA DIDÁCTICA

    OpenAIRE

    Isabel Gallardo Álvarez

    2010-01-01

    Impartir poesía en el aula ha sido, para muchos docentes, un trabajo difícil y a veces ininteligible. Ello puede deberse a la estructura y al contenido de este género lírico, que se especializa en emitir emociones y no en contar acciones o ideas. Es por eso que este artículo pretende darle herramientas al docente de Español, para que se adentre en el género lírico y pueda guiar al estudiantado a una lectura lúdica, dialógica, plurisignificativa y creativa, tal y como lo solicitan los programa...

  3. Cáncer de piel no melanoma: riesgos e itinerarios

    OpenAIRE

    Guillermo Sánchez Vanegas

    2012-01-01

    El cáncer de piel no melanoma (CNM) es una patología creciente en Colombia, producto de una serie de procesos biológicos, anclados en dinámicas historicosociales particulares. En este trabajo buscamos darle una mirada integral al problema, explorando las condiciones asociadas con el riesgo de padecer la enfermedad y estudiando las experiencias, con el sistema de salud, de las personas enfermas. Se llevó a cabo un diseño de metodología mixta y por fases, combinando un estudio de casos y co...

  4. Brujas: las mujeres escriben

    OpenAIRE

    1982-01-01

    Esta publicación es un espacio para darle voz a la mujer: una voz desde la narrativa en femenino a través de la literatura, el cuento o la poesía; a través del ensayo que reflexiona por sus derechos, por su participación en la política, por su ciudadanía. Una voz que cuestiona y rechaza las imposiciones del sistema patriarcal, los roles, el sexismo y las relaciones interpersonales basadas en la violencia y la subordinación. A través de los diferentes textos, sus autoras realizan una invitaci...

  5. Diseño de un sistema termoeléctrico aprovechando características geotérmicas

    OpenAIRE

    Muñoz Zurdo, Ricardo

    2013-01-01

    Este proyecto estudia y desarrolla el diseño e implementación de un generador termoeléctrico aprovechando las características geotérmicas de los emplazamientos que lo permiten. Para el proyecto se hizo una investigación exhaustiva de los lugares donde se pudiera desarrollar de buena manera dicho modelo así como darle una aplicación útil a la producción de ésta electricidad. También se desarrolló un modelo físico para demostrar la funcionalidad del generador termoeléctrico, parte fundame...

  6. Estudio de una forma de equilibrio

    OpenAIRE

    Ruiz Wilches, Belisario

    2010-01-01

    "Vamos a estudiar la forma de equilibrio que toma un cuerpo parcial o totalmente fluído, cuando gira alrededor de un eje que pasa por su centro de gravedad, estando sujeto, por una parte, a la atracción Newtoniana y por la otra, a una acción que tiende a darle una forma de equilibrio dinámico, diferente del estático. Estudiaremos solamente la curva que representa la sección meridiana del cuerpo, pues como la superficie de éste es engendrada por la rotación de dicha curva alrededor de un eje, ...

  7. Cuidados de enfermería en el paciente de revascularizacion miocárdica con circulación extracorpórea

    Directory of Open Access Journals (Sweden)

    Ruby Elizabeth Vargas-Toloza

    2004-12-01

    Full Text Available La presente revisión pretende mostrar la complejidad del trabajo de enfermería con los pacientes sometidos a cirugías cardiovasculares, donde el plan de atención antes y después del procedimiento hace parte del éxito y recuperación del paciente. Siendo los síndromes coronarios agudos, una de las primeras causas de muerte a nivel mundial, se ha hecho el mayor esfuerzo por darle solución a este problema como lo son la cirugía de revascularización miocardica con circulación extracorporea.

  8. ESTRATEGIAS DE MOTIVACIÓN DEL APRENDIZAJE PARA LOS ESTUDIANTES DE ELECTROTECNIA

    OpenAIRE

    Susana Prado; Karla Puerto López; Andrea Pinzón Montes

    2013-01-01

    Se muestra la experiencia de aplicar diversas herramientas didácticas en la enseñanza de Electrotecnia Aplicada. Siguiendo la tendencia de los nuevos enfoques de la educación de darle mayor protagonismo al estudiante en su proceso de formación se emplearon: experiencias prácticas para corroborar conceptos teóricos, software de cálculo para comprobar los ejercicios de gabinete y diferentes software de simulación para visualizar y analizar diferentes eventos. Este portafolio fue usado para mant...

  9. EL FUTURO DE APPLE: SWIFT VERSUS OBJECTIVE-C

    OpenAIRE

    Cristian González García; Jordán Pascual Espada B.; Cristina Pelayo G-Bustelo; Juan Manuel Cueva Lovelle

    2015-01-01

    Hace unos meses Apple presentó un nuevo lenguaje de programación para sus plataformas: Swift. Con Swift, Apple pretende atraer a los programadores de los lenguajes de programación basados en la sintaxis de C++ y darles una mayor abstracción, que con Objective-C, para que sea más fácil programar para las plataformas de Apple. Por estas razones, se hace necesario contrastar lo pretendido por Apple y realizar un estudio del lenguaje de programación a fin de contrastar su objetivo. Para ello, se ...

  10. A responsabilidade do pesquisador ou sobre o que dizemos Acerca da ética em pesquisa

    Directory of Open Access Journals (Sweden)

    Maria Itayra Coelho de Souza Padilha

    2005-01-01

    Full Text Available El presente estudio considera los cuidados éticos que el investigador debería tomar en cuenta durante el desarrollo de la investigación. Pretendemos y creemos conveniente presentar algunas reflexiones con relación a algunas cuestiones que actualmente provocan dudas entre los investigadores, asi también, sobre aquellas que relativamente parecen no provocar ninguna inseguridad, no obstante, estan siendo omitidas y dislocadas de sus bases teóricas, separadas de los principios y los valores las cuales deberian darle sustentación de forma a ser considerada como una simple tarea operacional.

  11. PSICOANÁLISIS Y PSICOLOGÍA EN LA OBRA DE LOUIS ALTHUSSER // PSYCHOANALYSIS AND PSYCHOLOGY IN LOUIS ALTHUSSER’S WORK

    OpenAIRE

    Matías Abeijón

    2013-01-01

    Este trabajo tiene como objetivo reconstruir el itinerario intelectual de la obra de Louis Althusser respecto a sus relaciones con el psicoanálisis y la psicología. De acuerdo con nuestra hipótesis, Althusser recurrió al psicoanálisis con el objetivo de darle un uso particular: impugnar las ciencias psicológicas y la noción burguesa de sujeto. Así, el psicoanálisis habría servido al desarrollo de sus conocidas críticas a la categoría de sujeto, en tanto este se presentaría como una alternativ...

  12. El rol de los gobernadores opositores en las elecciones presidenciales

    OpenAIRE

    Joel Sebastián Schneider

    2004-01-01

    Este trabajo efectúa un análisis de la forma en que los partidos de oposición actúan en las elecciones presidenciales, buscando darles un rol activo. Para ello se construye un modelo con dos niveles de gobierno, uno Nacional y el otro provincial o estadual. Estados Unidos, Argentina, Brasil y México, republicas presidencialistas federales, serán sometidas a un relevamiento empírico, que permitirá confirmar la hipótesis de que es más probable que cuando la oposición obtenga la presidencia, es ...

  13. Why Animals Can Make Us Better Victorianists

    OpenAIRE

    Moine, Fabienne

    2017-01-01

    From Elizabeth Barrett Browning’s devoted Flush and Lewis Carroll’s scurrying white rabbit to Anna Sewell’s wretched Black Beauty, Christina Rossetti’s monstrous crocodile and her brother’s darling wombat. From Dickens’s canine friends and Charles Darwin’s memorable finches, to mites under the microscope, pet cats on ladies’ laps and dinosaurs in Sydenham Park. From chimpanzees in menageries and hunting trophies on colonial walls to pedigree dogs at the Kennel Club and war dogs on the battlef...

  14. ATRACTIVO SEXUAL FEMENINO A LO LARGO DEL CICLO MENSTRUAL: ANÁLISIS BAJO LA PERSPECTIVA DE LA PSICOLOGÍA EVOLUTIVA

    OpenAIRE

    Georgina I. García-López

    2015-01-01

    Uno de los retos más importantes sobre los estudios en humanos es el de explicar los mecanismos fisiológicos que subyacen en el despliegue del comportamiento. La psicología evolutiva trata de establecer una relación de causa-efecto entre la forma en que se comporta el humano y los cambios fisiológicos que ocurren en él. A fin de darle una interpretación evolutiva a la conducta humana, en el presente trabajo se sometió a prueba la hipótesis que propone que los hombres perciben a...

  15. ESTUDIO DE LA PREPARACION DE CARBONES ACTIVADOS CON CARACTERISTICAS DE TAMIZ MOLECULAR

    OpenAIRE

    BELLO BUSTOS, GERMAN EDUARDO

    2004-01-01

    Se estudió la preparación de tamices moleculares de carbón a partir de carbonizados de madera de Eucalyptus globulus. Los tamices se prepararon mezclando un carbonizado en polvo con alquitrán mineral para darles forma de pellets, y sometiendo la mezcla a un tratamiento térmico en CO2- En la etapa de carbonización se estudió el efecto de la temperatura de carbonización de la madera en ¡as propiedades de tamiz de los materiales obtenidos. En la etapa de activación se estudiaron las ...

  16. Fallas de transparencia: hacia una incorporación efectiva de políticas de transparencia en las organizaciones públicas

    OpenAIRE

    David Arellano Gault

    2007-01-01

    La discusión sobre la apertura de la información gubernamental forma parte de un debate mucho más amplio: el de la transparencia como principio gen eral de una sociedad democrática. En México, el avance de esta agenda ha sido importante sin lugar a dudas. Sin embargo, este de bate está basado, muchas veces, en una especie de esperanza poco fundamentada teórica y analíticamente. Darle contenido a la categoría social "transparencia" pareciera fundamental, con el fin de dirigirse hacia agen d...

  17. Ukola Club. Bar americano

    Directory of Open Access Journals (Sweden)

    Azpiazu, J. R.

    1961-03-01

    Full Text Available En la calle de Serrano, aprovechando un semisótano dedicado a otro negocio anteriormente, se ha instalado un bar americano, de cuyo interior ofrecemos algunos pormenores. Se han cuidado, especialmente, las condiciones acústicas, resueltas por medio de un techo de escayola perforada, con vitrofib en su parte superior, y paredes de madera, que contribuyen a darle un ambiente cálido y acogedor. El soporte de hierro laminado existente en el centro del local, cuya supresión hubiera sido costosa, se ha revestido con lajas de mármol que le convierten en un elemento decorativo.

  18. Estudio in vitro del espesor radicular residual dejado por diferentes sistemas rotatorios en la zona de peligro

    OpenAIRE

    Luna Lara, Carlos Alberto

    2014-01-01

    INTRODUCCIÓN: La adecuada limpieza y conformación del conducto radicular consiste en darle una forma al conducto que permita remover el tejido pulpar vital o necrótico, dentina y microorganismos y facilitar la obturación tridimensional de esté. Uno de los principales retos de la endodoncia es la preparación de los conductos curvos ya que se pueden producir accidentes de procedimiento como: la transportación del conducto, fractura de instrumentos, adelgazamiento excesivo de las paredes del con...

  19. El Caribe: movimientos político-poblacionales, 1950-1994

    OpenAIRE

    Lilia Barragán A.

    2000-01-01

    Nuestro estudio pretende hacer hincapié no sólo en aquellas políticas violentas o adversas que motivan los desplazamientos de personas, sino también en cómo los factores económicos, que en muchos casos los podemos encontrar encubiertos por motivaciones políticas, también son motivo de desplazamiento forzado y cómo en muchos momentos son confundidos o encubiertos unos en los otros para no darles el valor y la categoría que les corresponde, como lo podemos observar en los casos de Haití y Cuba,...

  20. Diagnóstico y propuesta de negocio para el cultivo de mora orgánica en el municipio de El Colegio “Municipio Saludable y Polo de Desarrollo Local”

    OpenAIRE

    Parra Castañeda, Katherine Lorena; Burgos Maldonado, Gina Marcela; Castro Castro, Jorge Alberto

    2012-01-01

    El proyecto denominado “Municipio Saludable- Polo de Desarrollo Local” es un convenio entre la Universidad Del Rosario y la Gobernación de Cundinamarca, en el cual durante dos años algunos alumnos han contribuido con su conocimiento para plantear estrategias y alternativas aplicables para el desarrollo económico y social del Municipio de “El Colegio”. Gracias a éste proyecto surge la necesidad de darle continuidad a los planteamientos de estrategias y alternativas de desarrollo para el sector...

  1. Edificio de apartamentos, Cuba

    Directory of Open Access Journals (Sweden)

    Fernández, Emilio

    1961-07-01

    Full Text Available La obra consta de dos plantas iguales y en cada una de ellas se distribuyen dos apartamentos: uno de tres dormitorios, dos baños., portal, sala, comedor, cocina, patio de servicio y W.C. de servicio; otro, al fondo, con sala-comedor, dos dormitorios, cocina y patio de servicio. Los portales y patios de servicios han sido cerrados casi totalmente por medio de celosías, con objeto de darles un carácter privado y protegerlos de la exposición directa del sol, evitando así los inconvenientes de nuestro clima.

  2. SURVIVAL ANALYSIS OF CANCER PATIENTS USING PARAMETRIC AND NON-PARAMETRIC APPROACHES

    Directory of Open Access Journals (Sweden)

    M. AKRAM, M. AMAN ULLAH AND R. TAJ

    2007-10-01

    Full Text Available Exploring the health related quality of life is usually the focus of the survival studies. Using the health data of cancer registry in Multan, Pakistan, an investigation about the survival pattern of cancer patients was explored, using the non-parametric and parametric modeling strategies. The Kaplan-Meier method and Weibull model based on Anderson-Darling test were applied to the real life time data. Findings suggested different sex-superiority of survival pattern among different groups of cancer patients. Interestingly, Kaplan-Meier and Weibul model provided a very close estimate of the survival function and other characteristics of interest.

  3. Estudio de la cinética de gelatinización y de las variables operativas en el parbolizado de arroz

    OpenAIRE

    Ojeda, Carlos Alberto

    1998-01-01

    El proceso de parbolizado de arroz requiere de una etapa que gelatinice el almidón presente en el grano. Para lograrlo, muchos de los sistemas de uso industrial recurren al vapor de agua. La gelatinización del almidón tiene por objeto darle al arroz parbolizado sus peculiares características, tales como su alto rendimiento durante la molienda y sus propiedades culinarias: flavour, mayor dureza, menor pegajosidad, y mayor resistencia a la sobrecocción. La generación de vapor de agua acarrea un...

  4. Efecto del nivel de inclusión y concentración de vinaza de caña (Saccharum oficinarum) sobre los parámetros de fermentativos y calidad nutricional de un ensilaje de maralfalfa (Penissetum sp.)

    OpenAIRE

    Vargas Naranjo, Sergio Andrés

    2015-01-01

    Resumen: La vinazas producidas por la destilación del etanol, conciernen una severa preocupación ambiental debido a su alta cantidad de materia orgánica (100-130g/L), alta demanda bioquímica de oxígeno y la ausencia de opciones de plantas productoras de etanol para darle valor agregado a los subproductos. Por ello, hoy en día se reconocen propiedades nutricionales en las vinazas con potencialidad económica para aprovecharlas y direccionarlas en alimentación animal. El objetivo de este estudi...

  5. Manejo integral de residuos sólidos en la Escuela Nacional de Carabineros

    OpenAIRE

    Javier Raúl Romero Roa

    2012-01-01

    La Escuela Nacional de Carabineros de la Policía Nacional de Colombia, ubicada en el municipio de Facatativá, Cundinamarca, al hacer un diagnóstico sobre la situación del manejo de los residuos sólidos dentro de la institución, tuvo la iniciativa de optimizar sus condiciones ambientales; se mejoraron las condiciones para darle un manejo adecuado a los residuos sólidos que provenían de la Escuela. Ante estas circunstancias, se vio la necesidad de implementar un Plan de Manejo Integral de Resid...

  6. Metodología de planificación de auditoría interna con enfoque a riesgos :caso práctico, Banco Solidario S.A.

    OpenAIRE

    Arostegui Tamayo, Eliana Catalina

    2008-01-01

    Para entender, analizar, valorizar, gerenciar y decidir sobre la manera como adelantar la gestión corporativa frente al riesgo, la dirección de las entidades bancarias concibió con el apoyo de una unidad denominada “Administración de Riesgos” o “Gerencia de Riesgos”, que al final sólo es una función más, de muy alto nivel dentro de la jerarquía directiva, para darle paso a dicha necesidad; es así como se considera la Administración de Riesgos como un conjunto de estrategias que a partir de l...

  7. Desarrollo del mercado de cultivos organicos en el Ecuador con la producción del brocoli

    OpenAIRE

    Cassola Izaguirre, Aldo; Peralta Jurado, Gustavo; Pfister, Emilio

    2009-01-01

    En este trabajo nos hemos propuesto darle a conocer y llevar a cabo las ventajas que tiene consumir productos orgánicos creando una empresa dedicada a este tipo de producción con el cultivo del brócoli. Con la creación de esta empresa se diversifica la agricultura en nuestro país. El proyecto empieza dando a conocer las ventajas que tiene el consumir productos cultivados en forma orgánica y en especial el brócoli. Dar a conocer el alto valor nutritivo del brócoli como alimento. También ...

  8. El mercadeo de servicios en las bibliotecas públicas: ¿una herramienta que se usa? Marketing services in public libraries: a tool which is used?

    OpenAIRE

    Ruth Helena Vallejo Sierra; Luís Roberto Téllez

    2008-01-01

    Se presentan los resultados de la investigación realizada en las bibliotecas públicas de Colombia sobre el uso y la aplicación del mercadeo como filosofía de gestión. El desarrollo metodológico se abordó desde una investigación descriptiva que partió de tipificar las bibliotecas, lo cual permitió diagnosticarlas y darles un perfil para luego analizar cada uno de los elementos de la mercadotecnia de acuerdo con los resultados obtenidos. Se analizaron el sistema de información de mercadeo, los ...

  9. Alternativas tecnológicas que permitan la elaboración de productos conformados ricos en ácidos grasos poli-insaturados, a partir de una especie marina grasa sub-explotada (SARACA, Brevoortia aurea)

    OpenAIRE

    Pennisi Forell, Susana Carolina

    2013-01-01

    El presente trabajo de Tesis pretende realizar un aporte a la industria pesquera, en el desarrollo y utilización de especies marinas sin valor comercial. En este caso se busca darle utilidad a la especie conocida como saraca (Brevoortia aurea), la cual se pesca a lo largo del año y principalmente como fauna acompañante durante la zafra de la corvina rubia (Micropogonias furnieri); al no tener valor en el mercado se la descarta. Los volúmenes capturados son considerables por lo que se decidió ...

  10. Anti-Ageing Cultures, Biopolitics and Globalisation

    Directory of Open Access Journals (Sweden)

    Brett Neilson

    2011-11-01

    Full Text Available In March 2004, the author attended the Inaugural International Conference on Longevity at the Sydney Exhibition and Convention Centre in Darling Harbour. As a cultural researcher interested in the interactions between demographic shifts, capitalist globalisation and changing forms of political power, the prospect of a direct encounter with the debates and practices surrounding the burgeoning field of anti-ageing medicine promised a means to observe the complex cultural dynamics of population ageing at play. This article explores the discord the atuhor witnessed; a quarrel that, despite the march of technological advance, attests the ongoing conflict in the nexus where politics meets life.

  11. Mejora de la competencia socioemocional en alumnos con necesidades educativas especiales

    OpenAIRE

    Lauría Martínez, María Cristina

    2013-01-01

    Las competencias socioemocionales se incluyen, hoy día, en el ámbito de las habilidades sociales, un tema que se encuentra en pleno tratamiento, sobre todo en el ámbito escolar. Puesto que la sociedad en general está dando mucha importancia a estas habilidades, es también esencial darle importancia en los alumnos que presentan necesidades educativas especiales y diseñar una propuesta de intervención que lleve como línea principal el desarrollo de las competencias socioemocionales. Se presenta...

  12. Configuración constitucional de la jurisdicción penal militar en tiempo de paz. El caso de México

    OpenAIRE

    Sodi Cuellar, Ricardo

    2016-01-01

    La sentencia pronunciada en el caso Radilla Pacheco por la Corte Interamericana de Derechos Humanos, condenó a México por violaciones graves a los derechos humanos por hechos cometidos por el Ejército durante la “guerra sucia” en la década de 1970. La Suprema Corte de Justicia de la Nación, asumió el compromiso por parte del Estado mexicano de darle cumplimiento a la sentencia de la Corte de San José. Se ordenó la apertura del expediente varios 912/2010. Su contenido da inicio a un cambio sin...

  13. Historia de la animación

    Directory of Open Access Journals (Sweden)

    Juan Ruiz

    2015-01-01

    Full Text Available Desde hace varios años se ha venido trabajando en darle movimiento a las imágenes dibujadas o pintadas. Este artículo expone una síntesis del desarrollo de los dibujos animados desde que el hombre primitivo, impresionado por los movimientos de los animales que cazaba, plasmó estas acciones en las pinturas rupestres de las grutas de Lascaux en Francia, o las de Altamira en España, hasta los creados por la genialidad de Walt Disney.

  14. Estudio del comportamiento de pinturas acrílicas sobre tablero contrachapado tras envejecimiento artificial

    OpenAIRE

    ,; Osete Cortina, L.; Llamas-Pacheco, R.; Oliver-Villanueva, J.V.

    2017-01-01

    Entre los soportes más empleados en arte contemporáneo se encuentran los tableros derivados de la madera. Hasta el momento, las investigaciones para determinar la influencia de un soporte de tablero contrachapado en capas pictóricas acrílicas así como las condiciones óptimas para su conservación, resultan insuficientes o completamente ausentes. En la elaboración de estos tableros se utilizan adhesivos para darles consistencia pero la influencia de estos adhesivos en las capas pictóricas artís...

  15. Entornos personales de aprendizaje: cómo construir tu propio conocimiento

    OpenAIRE

    Ramos Fernández, Leonor

    2015-01-01

    Todos tenemos nuestro propio entorno personal de aprendizaje, desde la infancia. Ahora contamos con herramientas que nos ayudan a darle forma y nos sirven también para compartirlo con el resto de personas que están aprendiendo lo mismo que nosotros o que pueden compartir con nosotros lo que aprendieron. Pero el concepto de personal learning environment no se refiere solo a un software, sino a un nuevo enfoque sobre cómo podemos aprovechar al máximo la tecnología de la web 2.0 p...

  16. Detección y tratamiento del TDAH. Protocolo de actuación

    OpenAIRE

    Rogero Pascual, Andrea

    2016-01-01

    El Trastorno por Déficit de Atención e/y Hiperactividad es uno de los subtipos reconocidos por Ley desde la LOMCE, dentro del grupo de Alumnos con Necesidades Específicas de Apoyo Educativo (ACNEAE) y uno de los más frecuentes dentro de dicho grupo. Con este trabajo pretendemos informar y formar, especialmente al profesorado, acerca de las características de este tipo de alumnos y darles a conocer los principales indicadores que les permitan detectarlos lo antes posible, con el fin de pod...

  17. Fábrica de paños en Tunja, en el siglo XVIII

    Directory of Open Access Journals (Sweden)

    Sergio Elías Ortíz

    1968-05-01

    Full Text Available Con los pocos datos que se tiene respecto del desarrollo de la manufactura de tejidos en los tiempos coloniales, parece que pudiera corresponder a los señores Juan Manuel de Muelle y Pedro Hernández de Zurita el honor de haber sido los iniciadores en nuestra patria de la industria de textiles, ramo que ellos trataron de sacar del ámbito familiar para darle categoría de industria cuando menos regional, con carácter de fábrica para producción en masa y empleo de muchos brazos para las labores.

  18. La conformación del cáncer como objeto científico y problema sanitario en la Argentina: discursos, prácticas experimentales e iniciativas institucionales, 1903-1922

    OpenAIRE

    Buschini, José D.

    2014-01-01

    El trabajo analiza la conformación del cáncer como problema sanitario y como objeto científico en la Argentina en las dos primeras décadas del siglo XX. Se consideran la adquisición y circulación de conocimientos foráneos sobre el tema, el contexto en el que surgieron los primeros desarrollos experimentales, las iniciativas institucionales promovidas desde la profesión médica y el modo en que se logró interesar a las autoridades estatales y a la sociedad civil para darles curso. Se estudia de...

  19. The construction of cancer as an object of scientific study and sanitary problem in Argentina: discourses, experimental practices and institutional initiatives, 1903-1922

    OpenAIRE

    Buschini, Jose Daniel

    2017-01-01

    El trabajo analiza la conformación del cáncer como problema sanitario y como objeto científico en la Argentina en las dos primeras décadas del siglo XX. Se considera la adquisición y circulación de conocimientos foráneos sobre el tema, el contexto en el que surgieron los primeros desarrollos experimentales, las iniciativas institucionales promovidas desde la profesión médica y el modo en que se logró interesar a las autoridades estatales y la sociedad civil para darles curso. Se estudia de ma...

  20. Un depurador interactivo de programas para microprocesador i8086 PRO-EXE

    OpenAIRE

    Ordóñez Reinoso, Iván; Montalvo Ramírez, Luis; Valencia Pinargote, Galo; Ortega López, Fernando

    1989-01-01

    Se describe el diseño y desarrollo de un paquete de depuración de programas escritos en lenguaje de máquina del microprocesador i8086 en computadoras compatibles con la IBM PC bajo DOS. El paquete está enteramente escrito en lenguaje Assembly y en forma modular, y concebido para proporcionar una gran cantidad de información útil al usuario, además de darle opciones de manejo de archivos y de depuración de su programa, es decir, de ejecución de su código en varias modalidades. La interfaz con ...

  1. El sonido en el cine: percepción, historia, elementos y técnica

    OpenAIRE

    Pérez Mohedano, Ignacio

    2013-01-01

    El autor del proyecto se centra en un elemento en concreto del cine, el sonido, su definición elemental, sus características, la historia de su aparición y sus sucesivas aportaciones al mundo del séptimo arte sin desvincularse del punto (o contrapunto) visual al que está ligado. Trata, también, las características técnicas y los procesos de producción, grabación y postproducción que se llevan a cabo para darle forma

  2. Nutrigenómica y la nutrición personalizada

    OpenAIRE

    Vázquez Saldaña, Rocío

    2016-01-01

    Las bases de nuestra salud, bienestar y longevidad están muy relacionadas con la diversidad bioquímica de los alimentos que tomamos. La nutrición actualmente se apoya en diferentes ciencias como la bioquímica, biología molecular, genética y fisiología general. El cambio de pasar de una epidemiología clásica a darle un enfoque molecular está directamente relacionado con el hecho de que los efectos de los alimentos en la salud no pueden ser completamente entendidos sin una comprensión más profu...

  3. Estudio para la minimización del residuo de colillas de tabaco y su posible reutilización

    OpenAIRE

    MONZONIS MARCO, JOSE CARLOS

    2011-01-01

    Para poder comprender mejor la problemática que conlleva la realización de este estudio, en primer lugar debemos conocer la verdadera composición de los cigarrillos, las cantidades de los mismos que hay en el mundo y como los residuos que estos generan interaccionan con el medio que los rodea y pueden producir diferentes clases de contaminación. Al mismo tiempo se plantearan una serie de actuaciones para minimizar el impacto de estos residuos y darle una utilidad a los mismo...

  4. Principio de conservación de las fuentes de trabajo en la Ley de Quiebras : Una señal de la Corte a las empresas recuperadas

    OpenAIRE

    Amodio, Marcelo; Crosio, Amilcar

    2014-01-01

    La reforma de la ley de concursos y quiebras trajo aparejada la primera interpretación de la Corte Suprema de Justicia de la Nación en relación a la necesidad de darle prevalencia a los derechos de los trabajadores por sobre otros acreedores. Se trata del caso: CLINICA MARINI SA s. Quiebra CSJN, Fallos: C. 534; XLIV. 01/08/13; allí la Corte no solo rompe con el principio de paridad entre reclamantes, -dándole expresa prevalencia al trabajador- sino que reconoce la preservación de las fuentes ...

  5. Philadelphia (1993): las claves de un icono cultural

    OpenAIRE

    Aijón Oliva, José

    2005-01-01

    [ES] Philadelphia responde a la razón fundamental del cine comercial de los últimos tiempos: darle al público aquello que quiere ver. A medida que van surgiendo los problemas en la sociedad, el cine se interesa por ellos y realiza películas centradas en ese tema. A principios de los años 90 la alarma social ante el virus del SIDA era tan importante que surgieron muchas películas que trataban este tema. Philadelphia es la más popular de todas ellas y la más accesible (y probablemente la de may...

  6. UN PRIVILEGIO PARA EL ESTADO DE MICHOACÁN: SUS PUEBLOS MÁGICOS

    OpenAIRE

    Horacio Mercado Vargas; Edgar Allan Mercado Flores

    2013-01-01

    El Estado de Michoacán, que se ubica en la parte del extremo sur occidental de México, posee paisajes muy hermosos: de bosques, praderas y lagunas de gran belleza, montañas y volcanes que descienden hacia el mar. Es uno de los más ricos en recursos naturales. La Secretaría de Turismo, diversas entidades del gobierno federal y gobiernos estatales y municipales desarrollaron el Programa de Pueblos Mágicos, para darle un valor a ciertas poblaciones del país que tienen características culturales ...

  7. Enhancing pathogen identification in patients with meningitis and a negative Gram stain using the BioFire FilmArray(®) Meningitis/Encephalitis panel.

    Science.gov (United States)

    Wootton, Susan H; Aguilera, Elizabeth; Salazar, Lucrecia; Hemmert, Andrew C; Hasbun, Rodrigo

    2016-04-21

    Meningitis with a negative cerebrospinal (CSF) Gram stain represents a diagnostic and therapeutic challenge. The purpose of our study was to evaluate the performance of the BioFire FilmArray(®) Meningitis/Encephalitis (FA ME) panel in patients presenting with community-acquired meningitis with a negative Gram stain. CSF from 48 patients with community-acquired meningitis with a negative Gram stain admitted to four hospitals in Houston, TX underwent additional testing by the FA ME. FA ME results were compared to results obtained as part of routine evaluation. The panel detected pathogens not previously identified in 11 (22.9 %) of 48, but did not detect pathogens identified by standard technique (West Nile virus, Histoplasma) in 5 (15.2 %) patients. Rapid testing for the most common pathogens causing meningitis will aid in the diagnosis and treatment of patients with meningitis.

  8. Genotyping and Resolution of a Case of Osteomyelitis in a 16-Month-Old Boy of Hispanic/African American Ethnicity.

    Science.gov (United States)

    Wachira, Eunice; Tran, Kayla; Taylor, Sara; Hoger, Sally; Dunn, James

    2016-02-01

    Most cases of osteomyelitis in children are caused by Staphylococcus aureus, although Kingella kingae, various streptococci, and Salmonella species also underlie this condition. Organisms such as Mycobacterium, Histoplasma, and Cryptococcus are much less commonly identified as etiologic agents in osteomyelitis. This case report describes a 16-month-old boy of Hispanic/African American ethnicity who had extensive inflammation of and discharge from his right ankle. Imaging studies supported a diagnosis of osteomyelitis. Acid-fast bacillus (AFB) and routine wound cultures were ordered on the wound discharge. The AFB culture yielded a positive result for Mycobacterium bovis, and molecular diagnostic testing further genotyped the microorganism as Mycobacterium bovis, Bacillus Calmette-Guerin (BCG). Herein, we report a rare case of osteomyelitis that we believe resulted from a BCG vaccine that the patient had received outside the United States. © American Society for Clinical Pathology, 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  9. Evaluation of regional-scale hydrological models using multiple criteria for 12 large river basins on all continents

    Science.gov (United States)

    Huang, Shaochun; Krysanova, Valentina; Hattermann, Fred; Vetter, Tobias; Flörke, Martina; Samaniego, Luis; Arheimer, Berit; Yang, Tao; van Griensven, Ann; Su, Buda; Gelfan, Alexander; Breuer, Lutz; Haberlandt, Uwe

    2016-04-01

    A good performance of hydrological impact models under historical climate and land use conditions is a prerequisite for reliable projections under climate change. The evaluation of nine regional-scale hydrological models considering monthly river discharge, long-term average seasonal dynamics and extremes was performed in the framework of the ISI-MIP project for 12 large river basins on all continents. The modelling tools include: ECOMAG, HBV, HYMOD, HYPE, mHM, SWAT, SWIM, VIC and WaterGAP3. These models were evaluated for the following basins: the Rhine and Tagus in Europe, the Niger and Blue Nile in Africa, the Ganges, Lena, Upper Yellow and Upper Yangtze in Asia, the Upper Mississippi, MacKenzie and Upper Amazon in America, and Darling in Australia. The model calibration and validation was done using WATCH climate data for all cases. The model outputs were evaluated using twelve statistical criteria to assess the fidelity of model simulations for monthly discharge, seasonal dynamics, flow duration curves, extreme floods and low flow. The reproduction of monthly discharge and seasonal dynamics was successful in all basins except the Darling, and the high flows and flood characteristics were also captured satisfactory in most cases. However, the criteria for low flow were below the thresholds in many cases. An overview of this collaborative experiment and main results on model evaluation will be presented.

  10. Acidification of lake water due to drought

    Science.gov (United States)

    Mosley, L. M.; Zammit, B.; Jolley, A. M.; Barnett, L.

    2014-04-01

    Droughts are predicted to increase in many river systems due to increased demand on water resources and climate variability. A severe drought in the Murray-Darling Basin of Australia from 2007 to 2009 resulted in unprecedented declines in water levels in the Lower Lakes (Ramsar-listed ecosystem of international importance) at the end of the river system. The receding water exposed large areas (>200 km2) of sediments on the lake margins. The pyrite (FeS2) in these sediments oxidised and generated high concentrations of acidity. Upon rewetting of the exposed sediments, by rainfall or lake refill, surface water acidification (pH 2-3) occurred in several locations (total area of 21.7 km2). High concentrations of dissolved metals (Al, As, Co, Cr, Cu, Fe, Mn, Ni, Zn), which greatly exceeded aquatic ecosystem protection guidelines, were mobilised in the acidic conditions. In many areas neutralisation of the surface water acidity occurred naturally during lake refill, but aerial limestone dosing was required in two areas to assist in restoring alkalinity. However acidity persists in the submerged lake sediment and groundwater several years after surface water neutralisation. The surface water acidification proved costly to manage and improved water management in the Murray-Darling Basin is required to prevent similar events occurring in the future.

  11. La promoción de la salud en el contexto del desarrollo social

    Directory of Open Access Journals (Sweden)

    Enrique Guada

    2002-01-01

    Full Text Available En el marco del desarrollo social, la promoción de la salud pretende el logro del bienestar y la calidad de vida individual, familiar y comunitaria, reconciliando los intereses particulares con lo institucional y colectivo, incorporando la participación ciudadana en la gestión de salud. Ese ser humano íntegro, forma parte de un grupo familiar y es miembro de una comunidad, es el ser humano cotidiano dentro de su realidad social, en una búsqueda permanente de protagonizar su propio proceso de vida, desde la perspectiva de la salud integral y de un proyecto de salud comunitario. La noción moderna de salud está asociada a la prevención, más aún en los países en vías de desarrollo con enormes dificultades económicas para darle sustentabilidad a los procesos de desarrollo humano. La Promoción de la Salud toma cada vez mayor importancia para darle viabilidad a los procesos que buscan la superación de la pobreza en sus múltiples expresiones

  12. ¿Por qué la filosofía no debe ceder la tematización de los conceptos de organización y gerencia a las ciencias de la gestión y la economía?

    Directory of Open Access Journals (Sweden)

    Germán David Gómez Palacio

    2014-12-01

    Full Text Available En tiempos en los que la modernidad nos arroya con un progreso rampante, es necesario darle el alcance suficiente a las disciplinas que se ocupan de lo que constituye lo humano, a saber: la subjetividad y los afectos en el ámbito institucional. En esta dimensión, la organización y la gerencia no son ajenas a tales sucesos por lo tanto, en esa medida, la filosofía, así como otros dominios pertenecientes al conocimiento humano, no pueden ceder la tematización de conceptos sobre organización a otras ciencias. Esto nos debe lleva a pensar que cuestionar el progreso de las organizaciones favoreciendo ante todo el sujeto, no es ir en contra del desarrollo, de la ciencia, tampoco del capitalismo; de lo que se trata es de darle a lo humano el estatuto primordial que le corresponde dentro de ese conjunto. Tal perspectiva, además de ser una obligación para todas las ciencias humanas y sociales, es un imperativo ético frente al cual, ni las humanidades, ni los humanistas pueden ceder.

  13. Guidelines for inclusion: Ensuring Indigenous peoples' involvement in water planning processes across South Eastern Australia

    Science.gov (United States)

    Saenz Quitian, Alejandra; Rodríguez, Gloria Amparo

    2016-11-01

    Indigenous peoples within the Murray-Darling Basin have traditionally struggled for the recognition of their cultural, social, environmental, spiritual, commercial and economic connection to the waters that they have traditionally used, as well as their right to engage in all stages of water planning processes. Despite Australian national and federal frameworks providing for the inclusion of Indigenous Australians' objectives in planning frameworks, water plans have rarely addressed these objectives in water, or the strategies to achieve them. Indeed, insufficient resources, a lack of institutional capacity in both Indigenous communities and agencies and an inadequate understanding of Indigenous people's objectives in water management have limited the extent to which Indigenous objectives are addressed in water plans within the Murray-Darling Basin. In this context, the adoption of specific guidelines to meet Indigenous requirements in relation to basin water resources is crucial to support Indigenous engagement in water planning processes. Using insights from participatory planning methods and human rights frameworks, this article outlines a set of alternative and collaborative guidelines to improve Indigenous involvement in water planning and to promote sustainable and just water allocations.

  14. The role of integrative taxonomy in the conservation management of cryptic species: the taxonomic status of endangered earless dragons (Agamidae: Tympanocryptis in the grasslands of Queensland, Australia.

    Directory of Open Access Journals (Sweden)

    Jane Melville

    Full Text Available Molecular phylogenetics is increasingly highlighting the prevalence of cryptic species, where morphologically similar organisms have long independent evolutionary histories. When such cryptic species are known to be declining in numbers and are at risk of extinction due to a range of threatening processes, the disjunction between molecular systematics research and conservation policy becomes a significant problem. We investigate the taxonomic status of Tympanocryptis populations in Queensland, which have previously been assigned to T. tetraporophora, using three species delimitation approaches. The taxonomic uncertainties in this species-group are of particular importance in the Darling Downs Earless Dragon (T. cf. tetraporophora, which is ranked as an endangered 'species' of high priority for conservation by the Queensland Department of Environment and Heritage Protection. We undertook a morphological study, integrated with a comprehensive genetic study and species delimitation analyses, to investigate the species status of populations in the region. Phylogenetic analyses of two gene regions (mtDNA: ND2; nuclear: RAG1 revealed high levels of genetic divergence between populations, indicating isolation over long evolutionary time frames, and strongly supporting two independent evolutionary lineages in southeastern Queensland, from the Darling Downs, and a third in the Gulf Region of northern Queensland. Of the three species delimitation protocols used, we found integrative taxonomy the most applicable to this cryptic species complex. Our study demonstrates the utility of integrative taxonomy as a species delimitation approach in cryptic complexes of species with conservation significance, where limited numbers of specimens are available.

  15. Kajian Beberapa Uji Kenormalan dan Kaitannya dengan Asumsi Kenormalan pada Beberapa Uji Statistika

    Directory of Open Access Journals (Sweden)

    Agus Santoso

    2016-09-01

    Full Text Available T-test is proposed as a parametric test to evaluate mean of population(s.  Therefore, it is assumed that the data are normally distributed. Recently simulation based research reveal that t-test are robust against non-normality assumption, as long as the distribution of the data are symmetric. For that reason, it is interesting to evaluate the normality test that commonly used before the t-test is applied, particularly on its power and its suitability on symmetrical assumption of t-test on distribution of the data.  On this research, the evaluation on normality test was focused at several tests, which are Anderson Darling, Kolmogorov-Smirnov, Ryan-Joiner, and normality test based on skewness and kurtosis, wether the t-test was restricted in evaluation of mean of one population. The simulation reveals that, in general, Anderson-Darling has greater power than the other test in evaluating the normality of the distribution of the data, wether the normality test based on skewness and kurtosis have greater suitability on symmetrical assumption of t-test on distribution of the data. Hence, it is recommended that the normality test based on skewness and kurtosis are used to evaluate the symmetrical assumption of t-test on distribution of the data.

  16. An Ontological Vindication of Darl’s Existential Authenticity from a Heideggerian Point of View

    Directory of Open Access Journals (Sweden)

    Fazel Asadi Amjad

    2014-01-01

    Full Text Available Many existential theorists have discussed the contrasts between the notions of authentic and inauthentic lives, among whom Martin Heidegger (1889-1976 is very influential. The present paper aims at proving William Faulkner’s (1897-1962 main character, Darl, in his novel, As I Lay Dying (1930, authentic from a Heideggerian point of view. Once the theoretical grounds are laid in detail, the analysis begins with focusing on the individual character, goes on by ontologically individualizing him, and finally proves his existential nonconformity with emphasis on the affective side of his Being rather than its rational side. After unveiling his nonconformity, it will be revealed that this nonconformity is unique to him, and this fact is among the factors making him authentic from a Heideggerian point of view; for what Heidegger calls authentic existence is achieved only when a means is discovered to rescue the individual from doing “as One does” to doing as he chooses to do himself. Freedom and responsibility, which are expanded upon, are the means by which Darl proves himself authentic.

  17. Continental impacts of water development on waterbirds, contrasting two Australian river basins: Global implications for sustainable water use.

    Science.gov (United States)

    Kingsford, Richard T; Bino, Gilad; Porter, John L

    2017-11-01

    The world's freshwater biotas are declining in diversity, range and abundance, more than in other realms, with human appropriation of water. Despite considerable data on the distribution of dams and their hydrological effects on river systems, there are few expansive and long analyses of impacts on freshwater biota. We investigated trends in waterbird communities over 32 years, (1983-2014), at three spatial scales in two similarly sized large river basins, with contrasting levels of water resource development, representing almost a third (29%) of Australia: the Murray-Darling Basin and the Lake Eyre Basin. The Murray-Darling Basin is Australia's most developed river basin (240 dams storing 29,893 GL) while the Lake Eyre Basin is one of the less developed basins (1 dam storing 14 GL). We compared the long-term responses of waterbird communities in the two river basins at river basin, catchment and major wetland scales. Waterbird abundances were strongly related to river flows and rainfall. For the developed Murray-Darling Basin, we identified significant long-term declines in total abundances, functional response groups (e.g., piscivores) and individual species of waterbird (n = 50), associated with reductions in cumulative annual flow. These trends indicated ecosystem level changes. Contrastingly, we found no evidence of waterbird declines in the undeveloped Lake Eyre Basin. We also modelled the effects of the Australian Government buying up water rights and returning these to the riverine environment, at a substantial cost (>3.1 AUD billion) which were projected to partly (18% improvement) restore waterbird abundances, but projected climate change effects could reduce these benefits considerably to only a 1% or 4% improvement, with respective annual recovery of environmental flows of 2,800 GL or 3,200 GL. Our unique large temporal and spatial scale analyses demonstrated severe long-term ecological impact of water resource development on prominent

  18. Bridging the Gap between Scientific and Indigenous knowledge to Better Understand Social Impacts of Changing Rainfall Regimes

    Science.gov (United States)

    Lynch, A. H.; Joachim, L.; Zhu, X.; Hammer, C.; Harris, M.; Griggs, D.

    2011-12-01

    The Murray-Darling Basin incorporates Australia's three longest rivers and is important for an agricultural industry worth more than $9 billion per annum, a rich biodiversity of habitat and species, and the very life of its traditional owners. The complex and sometimes enigmatic relationships between modes of variability and Australian regional rainfall distribution means that reliable projections of future water availability remain highly uncertain. Persistent drought, with associated heat stress and high fire danger, and episodic flooding rains present further challenges. Indeed, recent extremes likely herald a tipping point for the communities and ecosystems that rely on the river system. The Barmah-Millewa region in the Murray-Darling Basin is the heart of Yorta Yorta Traditional Tribal Lands. The Yorta Yorta continue to assert their inherent rights to country and have shown through oral, documentary and material evidence, that their social, spiritual, economic and cultural links with country have never been broken. Current water policy and practice, highly contested community consultation processes, cross-border governance issues and a changing social landscape create in this region a microcosm for understanding the complex demands of economic, environmental and cultural security along the Murray-Darling Basin as the climate changes. New approaches to bridging the gap between scientific and Indigenous epistemologies have emerged in recent years, including for example ecosystem-based adaptation (Vignola et al. 2009) and the analysis of cultural water flows (Weir 2010). The potential for innovation using these approaches has informed a study that investigates how the deep knowledge of country of the Yorta Yorta people can be combined with state of the art climate science to develop a better understanding of the competing demands on water resources in the Barmah-Millewa region now and in the future. An important dimension of this collaborative work with the Yorta

  19. Leishmaniosis laríngea recidivante: un caso inusual en un paciente inmunocompetente tratado con corticoides Recidivant laryngeal leishmaniosis: an unusual case in an immunocompetent patient treated with corticosteroids

    Directory of Open Access Journals (Sweden)

    R. Casero

    2010-06-01

    Full Text Available La leishmaniosis es una parasitosis de evolución crónica; en Argentina, sus agentes etiológicos principales pertenecen al complejo Leishmania (Viannia braziliensis, habitualmente asociado a lesiones cutáneas y mucocutáneas. Informamos en este trabajo un caso de leishmaniosis laríngea en un hombre de 29 años procedente de Jujuy, quien a raíz de múltiples subdiagnósticos portaba esta parasitosis desde hacía 20 años. En el año 2008 este paciente consulta por disfonía crónica y trastornos en las vías aéreas superiores, refiere que fue sometido a terapias con tuberculostáticos, antifúngicos y corticoides desde 2002. Diferentes biopsias y fibroscopías revelaron los siguientes diagnósticos: laringitis granulomatosa inespecífica, laringitis compatible con tuberculosis, laringitis compatible con histoplasmosis, linfoma Natural Killer extraganglionar a células pequeñas. Finalmente, estudios realizados en nuestro hospital demostraron la presencia de una laringe granulomatosa en toda su extensión, amastigotes intra y extracelulares de Leishmania spp., ausencia de formas compatibles con Micobacterias e Histoplasma, y laringitis crónica vinculable a Leishmania spp. El paciente realizó tratamiento con antimoniato de N-metil-glucamina y demostró una muy buena evolución clínica tras ser examinado 2 meses después. Si bien la leishmaniosis laríngea como lesión única no es la presentación prevalente de esta zoonosis, su estudio amerita especial atención en pacientes tratados con corticoides, pues esto evitará un diagnóstico tardío y las mayores consecuencias asociadas a la morbimortalidad propia de esta parasitosis.Leishmaniosis is a chronic parasitic disease, which in Argentina is mainly caused by protozoa belonging to the Leishmania (Viannia braziliensis complex, leading to cutaneous and mucosal pathologies. We report a rare case of laryngeal leishmaniosis in a 29 year-old man from Jujuy province, Argentina, who had been

  20. Identificação de agentes infecciosos pulmonares em autópsias de pacientes com a síndrome da imunodeficiência adquirida Identification of infectious agents in the lungs in autopsies of patients with acquired immunodeficiency syndrome

    Directory of Open Access Journals (Sweden)

    Sanivia Aparecida de Lima Pereira

    2002-12-01

    Full Text Available As afecções pulmonares são freqüentes em indivíduos infectados pelo vírus da imunodeficiência humana. Neste trabalho, procurou-se identificar através de histoquímica e imunohistoquímica, agentes infecciosos nos pulmões de indivíduos portadores da síndrome da imunodeficiência adquirida (SIDA, autopsiados entre março de 1990 e julho de 2000 na FMTM. Fragmentos de pulmão de 40 indivíduos com SIDA autopsiados foram analisados histologicamente. Foram identificados agentes infecciosos em 34 (85% casos dos 40 analisados, sendo que bactérias foram encontradas em 22 (55% casos. Entre os agentes fúngicos o Pneumocystis carinii foi encontrado em oito (19,1% casos; Cryptococcus sp em quatro (9,5%, Histoplasma sp em dois (4,8% e Candida sp em um (2,4% caso. Detectou-se também associação entre Pneumocystis carinii, Citomegalovirus e Cryptococcus sp; CMV e Toxoplasma gondii. Em cinco casos, Candida sp, CMV e Pneumocystis carinii estiveram associados a bactérias. Entre as infecções não bacterianas, os fungos foram os agentes infecciosos mais isolados dos pulmões em autópsias de indivíduos com SIDA, sendo o Pneumocystis carinii o mais freqüente.Lung diseases are frequently observed in individuals infected with HIV. The aim of this study was to identify infectious agents in the lungs in the autopsied individuals with AIDS performed between march 1990 and july 2000 at the school of medicine (Uberaba- Brazil, using histochemical and immunohistochemical techniques. Analysis was made on lungs obtained from 40 individuals with AIDS. Infectious agents were observed in 34 (85% cases of the 40 analyzed. Regarding fungis, Pneumocystis carinii was found in 8 (19.1%cases; Cryptococcus sp in 4 (9.5%cases, Histoplasma sp in 2 (4.8%cases and Candida sp in 1 (2.4%case. Association of Pneumocystis carinii, Citomegalovirus and Cryptococcus sp, was observed in one case, and in another, the association of CMV, and Toxoplasma gondii. There were 5 cases

  1. CERN at Arles: LHC featured in prestigious photography festival

    CERN Multimedia

    Jordan Juras

    2011-01-01

    Six photographs of the LHC experiment are being featured in this year’s Rencontres d’Arles photography festival. Simon Norfolk’s series, The LHC: the spirit of enquiry, was chosen as part of an exhibition celebrating 30 years of photography at the New York Times Magazine.   Simon Norfolk’s series "The LHC: the spirit of enquiry" on display at the Rencontres d'Arles festival. The photographs were originally taken in October 2006, when Norfolk was sent on an assignment to a ‘little known’ laboratory in Switzerland. “When I came to CERN, nobody I knew had ever heard of the place,” explains Norfolk. “Everybody I spoke to when I came back said, ‘You’ve been where? You’ve done what?’” Kathy Ryan, New York Times Magazine photo editor, sent Norfolk to ‘capture something new’. He describes Ryan’s assignments...

  2. EL DEPARTAMENTO AUTÓNOMO DE PRENSA Y PUBLICIDAD; CONSTRUYENDO LA NACIÓN A TRAVÉS DEL CINE DOCUMENTAL EN MÉXICO (1937-1939)

    OpenAIRE

    Ruiz Ojeda, Tania Celina

    2013-01-01

    El cine en sus orígenes como novedad científica, fuego de artificio que deslumbró al mundo, comenzó a marcar un camino que definiría su trayectoria durante las próximas décadas. Si bien de manera casi inmediata supo encontrar su vocación de artífice y la magia y la fantasía parecieron dejar atrás a la ciencia, el cine supo darles un lugar a todos. Y es que es el asombro de lo que se muestra en la pantalla, el asombro de los que han presenciado aquellos millones de cuadros por s...

  3. A nonpedagogical teacher education?

    DEFF Research Database (Denmark)

    Kvols, Anja Madsen; Madsen, Peter Hougaard; Beck, Mette Holdsendorf

    of the students and thereby it seems to contribute to a change of this pedagogical an educational paradigm. In 2013 Danish teacher education was latest reformed into a model of performance assessment and a general focus on learning outcomes. Increased attention paid to output and assessment raises issues...... regarding changes in education – which seem to come from evaluations more than from educational and pedagogical theories (Gruschka, 2011). Moreover, the teacher education has increased its focus on both research-based academic training and professional training at the same time. Our study is double – 1...... characterizes teacher professionalism? (Subject studies? Pedagogical studies? Professional training? Academic studies? Bildung studies? Etc.) - What characterizes ‘pedagogy of teacher education’ (Darling-Hammond, 2006; Loughran, 2008; Zeichner, 2005)?...

  4. Arte contemporáneo y educación artística: Propuestas de trabajo para segundo ciclo de Educación Infantil

    OpenAIRE

    Gutiérrez Barreñada, María Montserrat

    2015-01-01

    El presente Trabajo Fin de Grado persigue principalmente darles a los más pequeños oportunidades para que experimenten el mundo que les rodea a través de los sentidos aprovechando el potencial de la Educación Artística. Además se defiende la idea de cómo ésta resulta ser un gran medio para desarrollar la creatividad y la flexibilidad de pensamiento, permitiendo así la libertad de expresión del niño y el fomento del espíritu crítico tan necesario en la sociedad en la que estamos inmersos....

  5. Incorporating uncertainties into economic forecasts: an application to forecasting economic activity in Croatia

    Directory of Open Access Journals (Sweden)

    Dario Rukelj

    2011-06-01

    Full Text Available In this paper we present a framework for incorporating uncertainties into economic activity forecasts for Croatia. Using the vector error correction model (VECM proposed by Rukelj (2010 as the benchmark model, we forecast densities of the variable of interest using stochastic simulations for incorporating future and parameter uncertainty. We exploit the use of parametric and non-parametric approaches in generating random shocks as in Garrat et al. (2003. Finally we evaluate the results by the Kolmogorov-Smirnov and Anderson-Darling test of probability integral transforms. The main findings are: (1 the parametric and the non-parametric approach yield similar results; (2 the incorporation of parameter uncertainty results in much wider probability forecast; and (3 evaluation of density forecasts indicates better performance when only future uncertainties are considered and parameter uncertainties are excluded.

  6. Reactions and Attitudes towards the Darul Arqam Movement in Southeast Asia

    Directory of Open Access Journals (Sweden)

    J.H. Meuleman

    2014-03-01

    Full Text Available This article discusses the various reactions and attitudes towards Darul Arqam in Southeast Asia, especially in mid-1994, when Darl Arqam became the object of heated debate between institutions and leaders who support the crackdown on Darul Arqam and who reject the action throughout the region. Within this context, the authors analyze the different establishments of various governments, religious organizations, other agencies and the general population in Malaysia, Indonesia, Thailand, and Singapore. The results of this analysis is not limited to the Darul Arqam issue alone, but showed various common traits of Islam in a number of regions and countries in Southeast Asia.Copyright (c 2014 by SDI. All right reserved.DOI: 10.15408/sdi.v3i1.813

  7. La ética de Johann Friedrich Herbart como estética en sentido formativo o de cómo abrirle un espacio de posibilidad a la educación

    OpenAIRE

    Runge Peña, Andrés Klaus

    2009-01-01

    El escrito presenta los aspectos que hacen que la propuesta de formación moral deHerbart tenga un anclaje en lo estético. Se busca señalar, en especial, que Herbart,por el hecho de darle un papel tan importante a la educación como camino hacia la moralidad, hace que su propia recepción de la ética kantiana entre en tensión con su pensamiento pedagógico, debido al trascendentalismo de aquélla, y opte, en consecuencia y como salida a dicha situación, por una postura estética como camino formati...

  8. El perfil del empresario del sector salud de Santa Marta (Colombia: 1990 - 2005

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Elías Caro

    2013-10-01

    Full Text Available Abordar estudios de desarrollo empresarial en el Departamento del Magdalena y en especial de su capital, el Distrito Turístico, Histórico y Cultural de Santa Marta, teniendo en cuenta el perfil del empresario de las entidades prestadoras de servicios de salud, es de imperiosa necesidad hacerlo desde la capacidad de emprendimiento y de los factores tanto endógenos como exógenos que permitieron el surgimiento, evolución y desaparición de las empresas; por ello, implica darle una ojeada de forma simultanea a aspectos históricos y teóricos, relacionados de una u otra forma con el espíritu empresarial, el desarrollo socioeconómico y el emprendimiento.

  9. ¿Qué pasa si Ecuador no firma el Tratado de Libre Comercio?

    Directory of Open Access Journals (Sweden)

    Falconí , Fander

    2006-01-01

    Full Text Available Este artículo examina la pertinencia o no de firmar el Tratado de Libre Comercio (TLC con los Estados Unidos, dado el estado actual de las negociaciones, el contexto político y los escenarios posibles en caso de no concretarse este acuerdo. Se concluye con la necesidad de darle un mayor contenido a la posibilidad de no firmar el TLC, en tanto que se requieren propuestas concretas para una inserción dinámica del país en el mercado mundial, para consolidar incluso sus relaciones con los Estados Unidos y, sobre todo, para construir una integración verdadera, que no sea simplemente la reedición de caducas prácticas integracionistas.

  10. México 2008: el año en que se vivió en peligro Mexico 2008: The Year of Living Dangerously

    OpenAIRE

    JOY LANGSTON; BERNARDO PÉREZ

    2009-01-01

    El trabajo muestra los obstáculos que tendrá que librar la administración si acaso quiere seguir contando con el apoyo de los votantes, como el combate al narcotráfico y los efectos de la crisis financiera de EUA en la economía nacional. También analiza el paquete de reformas con las que el Presidente Calderón pretende darle continuidad a sus políticas en el 2008: la judicial, que modernizaría el sistema de impartición de justicia; la energética, que modificaría el régimen de la empresa más i...

  11. Música del Magdalena Grande en el siglo XIX. Eulalio Meléndez

    Directory of Open Access Journals (Sweden)

    Guillermo Henríquez Torres

    2000-01-01

    Full Text Available "Los carnavales de Ciénaga son más antiguos que los de Barranquilla; de aquí salieron para allá. Antes la gente bien no bailaba; sólo el pueblo. En esta casa nació la sociedad de Ciénaga; se bailó por primera vez cuando vino Jorge Isaacs. Hicimos un baile y Eulalio Meléndez compuso El caimán. Él vino a esta casa de quince años, papá lo empleó como ayudante de farmacia y aquí aprendió música con el profesor José C. Alarcón, quien venía de Santa Marta a darle clases a mi hermana María"

  12. La precarización del trabajo desde una perspectiva sociocultural en un contexto fronterizo

    Directory of Open Access Journals (Sweden)

    Marlene Solís

    2014-01-01

    Full Text Available En este artículo se analiza la conformación del mercado de trabajo de la industria del vestido en Tijuana; se identifican los factores estructurales generadores de precariedad y, según las vivencias respecto a ella, se reconstruyen perfiles distintos de los y las trabajadoras. El objetivo de esta propuesta analítica es mostrar la manera en que se entrelazan las escalas micro, meso y macrosocial reproduciendo o produciendo las condiciones de precariedad que prevalecen en los empleos de la industria de la confección en Tijuana. Para interpretar la experiencia de la precariedad, se aborda su dimensión sociocultural, al darle un papel central a los significados que le otorgan las personas al trabajo.

  13. ¿TIENE SENTIDO LA VIOLENCIA, EN UN SISTEMA ÉTICO DONDE NO EXISTE EL MAL? UNA MIRADA SOBRE LA POSICIÓN ÉTICA DE BARUCH DE SPINOZA (1632-1677

    Directory of Open Access Journals (Sweden)

    José Luis Da Silva Pinto

    2008-04-01

    Full Text Available Dentro de los linderos de un mundo que se desvela por explicarlo todo desde una racionalidad portadora de principios capaces de darle sentido a las causas y sus efectos, como también del tupido entramado de relaciones que tocan por igual a personas y cosas, tenemos que es de vital importancia puntualizar el lugar que ontológicamente posee el bien y la maldad. Para Baruch de Spinoza este era un problema de primer orden que debería ser abordado por el discurso filosófico, porque ayudaría a definir el rol del entendimiento, de las pasiones y con ello la factibilidad de un mundo capaz de ofrecer respuestas a las agresiones y violencias acostumbradas a maniobrar bajo el amparo del poder religioso, político y social de turno.

  14. Manejo integral de residuos sólidos en la Escuela Nacional de Carabineros

    Directory of Open Access Journals (Sweden)

    Javier Raúl Romero Roa

    2012-01-01

    Full Text Available La Escuela Nacional de Carabineros de la Policía Nacional de Colombia, ubicada en el municipio de Facatativá, Cundinamarca, al hacer un diagnóstico sobre la situación del manejo de los residuos sólidos dentro de la institución, tuvo la iniciativa de optimizar sus condiciones ambientales; se mejoraron las condiciones para darle un manejo adecuado a los residuos sólidos que provenían de la Escuela. Ante estas circunstancias, se vio la necesidad de implementar un Plan de Manejo Integral de Residuos Sólidos, que mejorara la separación en la fuente permitiendo un aprovechamiento del material reciclable, con el fin de reincorporar estos residuos al ciclo productivo, de tal forma que se disminuyeran los residuos que son dispuestos en el relleno regional.

  15. Neobarrocos, objetivistas, epifánicos y realistas: nuevos apuntes para la historia de la nueva poesía argentina

    Directory of Open Access Journals (Sweden)

    Martín Prieto

    2007-07-01

    Full Text Available Si tuviéramos que darle una « fecha de inicio » a la « nueva » poesía argentina —el adjetivo, « nueva », claro está, es provisorio, obligadamente perecedero y, aun en este caso, un poco anacrónico, toda vez que estamos hablando de algo que empezó hace ya más de veinte años—, aun a conciencia de que esa fecha será una línea trazada sobre la corriente de un río cuyo mismo movimiento corregirá, y dejando de lado, por lo menos por esta vez, los fastidiosos « antecedentes » (pero necesarios si pen...

  16. Algunas cuestiones sobre la enseñanza y el aprendizaje de las matemáticas en los estudios de economía y empresariales

    OpenAIRE

    Juan Manuel Sánchez-Quinzá; Antonio Sarmiento

    2002-01-01

    En este trabajo se pretende hacer un estudio de la situación actual de la enseñanza de las matemáticas en los primeros cursos de los estudios de económicas y empresariales. En lugar de darle un enfoque externo y objetivo, comparar lo que se estudia y como se estudia antes de la universidad y en la universidad, se ha optado por entrar en temas cognitivos más próximos a los que estudia la educación matemática. La investigación en educación matemática se desarrolló de modo espectacular a pa...

  17. Vivienda Social y Violencia Intrafamiliar: Una Relación Inquietante. ¿Una Política Social que Genera Nuevos Problemas Sociales?

    Directory of Open Access Journals (Sweden)

    Ana Sugranyes

    2005-01-01

    Full Text Available El artículo hace presente que la producción de viviendas de laactual política de Estado ha permanecido con un bajo perfil deinnovación, llegando a una situación que amerita conurgencia un mejoramiento radical. Ello ocurre por lapermanencia de formas de licitación de construcciones,postulaciones, asignaciones, etc., donde las características delos agentes participantes permanecen estratificadas, dando lasensación de que todo marcha bien y en perfecto orden. Acontrapunto de lo anterior, se hacen ver los impactos socialesnegativos atribuibles a la calidad del hábitat producido, lo quereafirma la necesidad de darle un vuelco renovador a lapolítica habitacional estatal.

  18. Principales prácticas de recursos humanos de las PyMEs industriales exitosas.

    Directory of Open Access Journals (Sweden)

    Liliana Rocío López Suárez

    2013-04-01

    Full Text Available El éxito competitivo de la PyME depende en parte de la buena gestión de recursos humanos,por lo que no darle el valor que requiere constituye una de las principales causas de fracasode estas empresas. La responsabilidad de la gestión eficaz de los recursos humanos recae fundamentalmenteen los directivos. La presente investigación tiene como objetivo identificarlos estilos de dirección y las principales prácticas de recursos humanos que llevan a cabo lasPyMEs industriales exitosas de México y España. Para alcanzar los objetivos antes reseñados,se ha realizado un análisis documental, así como entrevistas en profundidad a expertosen el área de recursos humanos. En este trabajo se presentan los principales resultados conrespecto a las prácticas mencionadas.

  19. Jean-François Sirinelli, Les baby-boomers. Une génération 1945-1969

    OpenAIRE

    Pellistrandi, Benoît

    2009-01-01

    Si para el demógrafo, el aumento de la natalidad y la proporción creciente de jóvenes en el conjunto de la población permiten señalar un fenómeno de «rejuvenecimiento» de la sociedad, el historiador no quiere limitarse a tener en cuenta estos datos sino que pretende darles significado a través de la «identidad» social y cultural que pueda adquirir tal generación —«[…] dans le processus de montée en puissance des jeunes au sein des sociétés occidentales de l’après-guerre, le poids démographiqu...

  20. Control Servo-Visual de un Robot Manipulador Planar Basado en Pasividad

    Directory of Open Access Journals (Sweden)

    Carlos Soria

    2008-10-01

    Full Text Available Resumen: En este trabajo se diseña un controlador servo visual basado en la propiedad de pasividad del sistema visual. Se propone un regulador con ganancias de control variables, de tal manera que se evita la saturación de los actuadores y al mismo tiempo presenta la capacidad de corregir errores de pequeña magnitud. Asimismo el diseno se hace tenieñdo en cuenta el desempeño L2, a fin de darle capacidad de seguimiento de objetos en movimiento, con un error de control pequeño. Se muestran resultados experimentales realizados en un robot manipulador industrial tipo planar para verificar el cumplimiento de los objetivos del controlador propuesto. Palabras Clave: robot manipulador industrial, control servo visual, control no lineal, pasividad

  1. Una pequeña aproximación al principio de veracidad en las denominaciones de origen e indicaciones de procedencia.

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Bonilla Sabogal

    2004-01-01

    Full Text Available La veracidad se ha catalogado como un principio de vital importancia en la propiedad industrial. Las denominaciones de origen y las indicaciones de procedencia son 2 figuras que han venido siendo muy desarrolladas principalmente en la legislación española y la francesa; a nivel suramericano la decisión 486 de la comunidad andina de naciones se ha encargado de darle un tratamiento innovador para los países del continente. Es por esto que, en el presente artículo se hace un estudio de estas dos figuras desde la perspectiva del principio de veracidad, lo cual creemos será vital importancia para los productores en cumplimiento de sus deberes de otorgamiento de garantía sobre la calidad del producto y de información específica sobre el origen y cualidades geográficas del mismo.

  2. SISTEMA DE FALLAS DE CALIDAD: ESTUDIO DE CASO CON UN ENFOQUE DE GESTIÓN DEL CONOCIMIENTO

    Directory of Open Access Journals (Sweden)

    Gerardo Sánchez Schmitz

    2004-03-01

    Full Text Available

    El presente estudio de caso se desarrolló en el Departamento de Calidad en una de las empresas comercializadoras de carne más importantes de México, ubicada en Hermosillo, Sonora, al noroeste de México. El objetivo de este trabajo es presentar la solución a un problema de manejo de información de fallas de calidad documentadas en el Departamento de Control de Calidad, utilizando el enfoque de gestión del conocimiento mediante el desarrollo de una memoria organizacional, para darle una solución más rápida a las reclamaciones de los clientes (internos y externos.

  3. Los derechos económicos en una sociedad decente y en teorías de la justicia

    Directory of Open Access Journals (Sweden)

    María del Rosario Guerra González

    2015-01-01

    Full Text Available En este artículo se demuestra mediante un análisis documental, con metodología hermenéutica, que los derechos económicos tienen una base ética pero que son más que esto, porque pueden reclamarse en los aspectos nacional, regional e internacional, a pesar de ser progresivos. La contribución de la investigación es subsanar errores en el tema de derechos económicos, situarlos en una "sociedad decente" y mostrar posibilidades y límites de las teorías sobre la justicia de John Rawls, Thomas Scanlon y Amartya Sen, con respecto a este tema. Se proponen nuevos derechos para darles mayor eficacia a los existentes.

  4. Truticultura y el excursionista en áreas rurales.

    Directory of Open Access Journals (Sweden)

    Celia Guzmán Hernández

    2013-01-01

    Full Text Available La condición actual en algunos bosques mexicanos deriva de una historia de depredación socioeconómica y física; sin embargo en los espacios naturales donde el impacto no fue tan severo, la truticultura ha logrado establecerse como una actividad importante en el ramo pecuario, cuidando de manera constante tanto la calidad de las aguas como la salud animal por los comités de fomento. Desde los años setenta, los pequeños propietarios y ejidatarios han encontrado una alternativa de subsistencia representada por el producto y la posibilidad de darle un valor agregado a sus paisajes, incorporando los servicios restauranteros y recreativos con la finalidad de atender a los excursionistas; empero el aprovechamiento de los mantos acuíferos en los bosques representa amenazas diversas y cambiantes al paso de los años.

  5. Akciğer rezeksiyonu sonrası gelişen ampiyem ve septik şok tablosu (Olgu sunumu)

    OpenAIRE

    Gürsu, Soner; Sırmalı, Mehmet; Aydın, Ertan; Yazıcı, Ülkü; Güven, Tayfun; Fındık, Göktürk; Kaya, Sadi

    2009-01-01

    SüleymanDemirel Üniversitesi TIP FAKÜLTESİ DERGİSİ: 2004 Aralık; 10(4) Akciğer rezeksiyonu sonrası gelişen ampiyem ve septik şok tablosu (Olgu sunumu) Soner Gürsu, Mehmet Sırmalı, Ertan Aydın, Ülkü Yazıcı, Tayfun Güven, Göktürk Fındık, Sadi Kaya, Özet Onaltı yaşında bronşektazi tanısıyla sol alt lobektomi ve lingulektomi uygulanan olgu taburculuğundan 19 gün sonra ateş, nefes darlığı ve ağızdan kötü koku gelmesi şikayetleriyle başvurdu. Hastaneye yatır...

  6. Distributions of time averages for weakly chaotic systems: the role of infinite invariant density.

    Science.gov (United States)

    Korabel, Nickolay; Barkai, Eli

    2013-09-01

    Distributions of time averaged observables are investigated using deterministic maps with N indifferent fixed points and N-state continuous time random walk processes associated with them. In a weakly chaotic phase, namely when separation of trajectories is subexponential, maps are characterized by an infinite invariant density. We find that the infinite density can be used to calculate the distribution of time averages of integrable observables with a formula recently obtained by Rebenshtok and Barkai. As an example we calculate distributions of the average position of the particle and average occupation fractions. Our work provides the distributional limit theorem for time averages for a wide class of nonintegrable observables with respect to the infinite invariant density, in other words it deals with the situation where the Darling-Kac-Aaronson theorem does not hold.

  7. Public speaking: creating presentations that are awe inspiring not yawn producing.

    Science.gov (United States)

    DeVan, Marsheila; Baum, Neil

    2013-01-01

    Many physicians want to be good platform speakers; however, few of us have the background and training necessary to be an outstanding public speaker. Yes, we all have slide decks and PowerPoint programs provided to us so that we can turn our backs to the audience and read the verbiage on the slides. But does that technique generate useful information to the audience? Or if speaking to a lay audience, does that kind of talk make the audience members get out their cell phones and call the office to make an appointment in our practice? Probably not. This article will provide suggestions that will enhance your speaking and presentation skills. This, in turn, will make you a darling of the pharma industry and encourage potential patients in your audience to become patients in your practice.

  8. Hacia una fenomenología de la enfermedad = Towards a phenomenology of disease

    Directory of Open Access Journals (Sweden)

    Estrada, Diego Alejandro

    2012-07-01

    Full Text Available La medicina moderna, al darle un carácter pasivo al cuerpo, lo condenó a la ausencia. Según esto, el hombre está compuesto de una realidad orgánica que deviene cosa, un objeto más de la naturaleza que puede ser controlado. Al igual que todo lo perecedero y lo temporal, el cuerpo es despreciado. Querer prolongar la vida, huirle a la muerte así sea de una forma técnica, es solo la muestra del afán por mantener lo corporal en unos indicadores artificiales de normalidad. Hacer una fenomenología de la enfermedad implica tener presente el asunto del cuerpo. Por medio de ella se constata un asunto que, a los ojos del mundo antiguo, era esencial: los hombres son mortales; por eso es preciso que se cuiden, que se cultiven a sí mismos, que se curen.

  9. Primaveras, Reproducciones y Ritualidades: reconstruyendo el universo simbólico de la Semana Santa

    Directory of Open Access Journals (Sweden)

    Orlando Durango Rueda

    2013-12-01

    Full Text Available Revisar el asiento de la cultura Caribe colombiana significa también darle el lugar a lo que parece ser la fuente de constitución y afirmación de esa misma cultura, procurando buscar en el tiempo sus reproducciones, hasta tener una forma económica y sociopolítica que la conforma y le permite la comprensión de todo el tejido que la define. Recogiendo el interés metodológico de un productor social como Antonio Gramsci, que pese a la contingencia del tiempo que pasa, mantiene la vigencia de sus consideraciones frente a la posibilidad de interpretación de lo social, parece procedente desde allí mirar la cultura Caribe como constructo de poder que se mantiene en la medida que dicho poder se disemina por el cuerpo de toda la sociedad.

  10. Juan Valdez la estrategia detrás de la marca

    Directory of Open Access Journals (Sweden)

    Ana María Parente

    2017-10-01

    Full Text Available El libro Juan Valdez, la estrategia detrás de la marca busca explicar la táctica de valorización del café colombiano desarrollada por la Federación Nacional de Cafeteros (FNC, que tiene como principal objetivo beneficiar a los caficultores colombianos a lo largo de la cadena de valor. Para ello, el texto hace un recuento del contexto del mercado cafetero mundial, haciendo énfasis en los principales proveedores y consumidores hasta finalmente centrarse en la construcción de la marca Café de Colombia® y Juan Valdez® como estrategias para darle competitividad al grano colombiano.

  11. Mediated transitions between CPD-activities & teaching and collaboration at local schools

    DEFF Research Database (Denmark)

    Nielsen, Birgitte Lund

    QUEST. Autonomous initiatives also indicated collaborative agency. The quite distant leadership at this school can be seen as a “black swan”. In general visible leadership and participation in team meetings has been important. The results indicate that teachers’ individual and social learning can......, 2004; Timperley, 2011). Extant research suggests a broad consensus pertaining to the core features of effective CPD, which include content focus, active learning, coherence, duration, collaborative activities and collective participation (Desimone, 2009; van Driel, Meirink, van Veen, & Zwart, 2012......, Bolam, McMahon, Wallace & Thomas, 2006). The overall purpose is to develop a sustainable model for CPD, supporting professional capital and bottom up development (Darling-Hammond, 2005; Heargreaves & Fullan, 2012). Primary and lower secondary teachers participated in one or more of 4 half-year modules...

  12. La definición jurídica del fenómeno metropolitano en el ámbito subnacional mexicano

    Directory of Open Access Journals (Sweden)

    Alberto Arellano Ríos

    2014-10-01

    Full Text Available En este texto se analiza la definición jurídica del fenómeno metropolitano en seis Estados de la república mexicana. Se estudian seis legislaciones estatales creadas expresamente para atender y darle forma jurídica a un problema que se ha acentuado con mayor fuerza en México, el cual desde el punto de vista de las instancias gubernamentales tiene mayor relevancia en los últimos veinte años. De este modo, el documento esboza el fenómeno metropolitano como contexto social. Luego traza los mecanismos constitucionales contemplados para gestionar y gobernar las zonas metropolitanos para, finalmente, exponer los casos estatales que ante la ausencia de un marco legal nacional, y desde la lógica del sistema federal, intentan sentar bases jurídicas para resolver este problema.

  13. LA POESÍA EN EL AULA: UNA PROPUESTA DIDÁCTICA

    Directory of Open Access Journals (Sweden)

    Isabel Gallardo Álvarez

    2010-01-01

    Full Text Available Impartir poesía en el aula ha sido, para muchos docentes, un trabajo difícil y a veces ininteligible. Ello puede deberse a la estructura y al contenido de este género lírico, que se especializa en emitir emociones y no en contar acciones o ideas. Es por eso que este artículo pretende darle herramientas al docente de Español, para que se adentre en el género lírico y pueda guiar al estudiantado a una lectura lúdica, dialógica, plurisignificativa y creativa, tal y como lo solicitan los programas vigentes de Español en Costa Rica. Por ello, se presenta un ejemplo de análisis, usando el poema Penélope de Marta Rojas que pretende ser un modelo para discutir y mejorar en el aula de Español.

  14. Take a Closer Look:Biofuels Can Support Environmental, Economic and Social Goals

    Energy Technology Data Exchange (ETDEWEB)

    Dale, Bruce E. [Michigan State University, East Lansing; Anderson, James [Ford Motor Company; Brown, Dr. Robert C. [Iowa State University; Csonka, Steven [Commerical Aviation Alternative Fuels Initiative (CAAFI); Dale, Virginia H [ORNL; Herwick, Gary [Transportation Fuels Consulting; Jackson, Randall [University of Wisconsin; Johnson, Kristen [Office of Science, Department of Energy; Jordan, Nicholas [University of Minnesota; Kaffka, Stephen R [University of California, Davis; Kline, Keith L [ORNL; Lynd, Lee R [Thayer School of Engineering at Dartmouth; Malmstrom, Carolyn [Michigan State University, East Lansing; Garlock, Rebecca [Michigan State University, East Lansing; Richard, Tom [Pennsylvania State University; Taylor, Caroline [Energy Biosciences Institute (EBI), Berkeley, California; Wang, Mr. Michael [Argonne National Laboratory (ANL)

    2014-07-01

    The US Congress passed the Renewable Fuels Standard (RFS) seven years ago. Since then, biofuels have gone from darling to scapegoat for many environmentalists, policy makers, and the general public. The reasons for this shift are complex and include concerns about environmental degradation, uncertainties about impact on food security, new access to fossil fuels, and overly optimistic timetables. As a result, many people have written off biofuels. However, numerous studies indicate that biofuels, if managed sustainably, can help solve pressing environmental, social and economic problems (Figure 1). The scientific and policy communities should take a closer look by reviewing the key assumptions underlying opposition to biofuels and carefully consider the probable alternatives. Liquid fuels based on fossil raw materials are likely to come at increasing environmental cost. Sustainable futures require energy conservation, increased efficiency, and alternatives to fossil fuels, including biofuels.

  15. Gomas trituradas: Estado del arte, situación actual y posibles usos como materia prima en Puerto Rico

    OpenAIRE

    Boero, Jorge; Valentín, Milton; Suárez, Oscar; Santos, Jeannette; Acosta, Felipe; Cáceres, Arsenio; Pando, Miguel

    2005-01-01

    La acumulación de neumáticos desechados es uno de los problemas ambientales que más importancia ha tomado en los últimos años. En países desarrollados la producción es mayor cada año y los métodos más usados para la disposición final, como la colocación en vertederos, ya no son permitidos. Esto genera la necesidad de buscar nuevas alternativas para darle una disposición apropiada a los neumáticos desechados. En Puerto Rico el problema debe ser atendido con prontitud, ya que se ha llegado a un...

  16. The transnational appeal of Danish TV series

    DEFF Research Database (Denmark)

    Jensen, Pia Majbritt

    Over the last five years, the near global reach of Danish TV drama has made Denmark the darling of the international TV industry and made Danish broadcasters and producers benefit both financially and status-wise. This is not just unprecedented. It is also interesting from an academic point of view...... because it challenges existing theories on global media geography, import/export of audio-visual content, transnational media reception and the importance of transnational TV viewing. According to these theories, non-Anglophone audio-visual content rarely exports outside its geo-linguistic region...... – in Denmark’s case the Nordic region – because audiences in other regions would be too far removed culturally and linguistically, and hence feel alienated Similarly, theories on the consumption of audio-visual content have neglected transnational, ‘non-resident’, viewing – i.e. when audiences engage...

  17. Global Monthly Water Scarcity: Blue Water Footprints versus Blue Water Availability

    Science.gov (United States)

    Hoekstra, Arjen Y.; Mekonnen, Mesfin M.; Chapagain, Ashok K.; Mathews, Ruth E.; Richter, Brian D.

    2012-01-01

    Freshwater scarcity is a growing concern, placing considerable importance on the accuracy of indicators used to characterize and map water scarcity worldwide. We improve upon past efforts by using estimates of blue water footprints (consumptive use of ground- and surface water flows) rather than water withdrawals, accounting for the flows needed to sustain critical ecological functions and by considering monthly rather than annual values. We analyzed 405 river basins for the period 1996–2005. In 201 basins with 2.67 billion inhabitants there was severe water scarcity during at least one month of the year. The ecological and economic consequences of increasing degrees of water scarcity – as evidenced by the Rio Grande (Rio Bravo), Indus, and Murray-Darling River Basins – can include complete desiccation during dry seasons, decimation of aquatic biodiversity, and substantial economic disruption. PMID:22393438

  18. Controls on Extreme Droughts and Adaptation Strategies in Semiarid Regions

    Science.gov (United States)

    Scanlon, B. R.; Cook, C.; Fernando, D. N.; LeBlanc, M.

    2012-12-01

    Increasing vulnerability to droughts with reduced per capita water storage, particularly in semiarid regions, underscores the need for predictive understanding of drought controls and development of adaptation strategies for water resources management. In this study we evaluate causes of major droughts in southwest and southcentral US (California and Texas) and southeast Australia (Murray Darling Basin). Impacts of climate cycles (ENSO, PDO, AMO, NAO, IOD) and atmospheric circulation on drought initiation and persistence are examined. Effects of drought on surface water reservoir storage, groundwater storage, irrigation, and crop production are compared. Adaptation strategies being evaluated include water transfers among sectors, particularly from irrigated agriculture to other groups, increasing storage using managed aquifer recharge, water reuse, and development of new water sources (e.g. seawater desalination). It is critical to develop a broad portfolio of water sources to increase resilience to future droughts.

  19. From sex strangler to model citizen: Mexico's most famous murderer and the defeat of the death penalty.

    Science.gov (United States)

    Meade, Everard

    2010-01-01

    Gregorio Cárdenas Hernández was Mexico's most infamous serial killer. After he confessed to killing four young women and burying them behind his home, he became the darling of the crime pages and criminological experts alike, and his case provoked a lively debate over the reinstatement of the death penalty in congress. The following essay uses his story, the policy debates it provoked, and his broader institutional odyssey in La Castañeda mental asylum (1943–1947) and Lecumberri prison (1948–1976) to explore how issues that affected Mexicans across the social spectrum were discussed and settled in a political system that was neither a dictatorship nor a democracy.

  20. Uncanny Valley Project, una intervención ciberfeminista en la X Bienal Centroamericana

    Directory of Open Access Journals (Sweden)

    Susan Campos Fonseca

    2017-06-01

    Full Text Available Se reflexiona sobre la intervención ciberfeminista Uncanny Valley Project en la X Sonora de la Bienal Centroamericana (2016. Se analiza desde una “proximidad crítica, corporal y feminista”, bajo la hipótesis del uncanny valley de Masahiro Mori, para exponer cómo ésta actúa sobre un presente en crisis, junto con la música electrónica en tanto vía para construer cuerpos de invención e innovación. Además, se explora la performatividad y la estética robótica practicadas por humanos dedicados a la música electrónica. El resultado permite identificar el fenómeno dentro de la música electrónica costarricense y darle visibilidad en el contexto centroamericano.