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Sample records for hill sobre aedes

  1. Dominância de Aedes aegypti sobre Aedes albopictus no litoral sudeste do Brasil Dominance of Aedes aegypti over Aedes albopictus in the southeastern coast of Brazil

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    Ricardo A Passos

    2003-12-01

    Full Text Available OBJETIVO: Analisar a infestação de Aedes aegypti e Aedes albopictus e verificar sua associação com fatores climáticos e com a sua freqüência em recipientes de área urbana. MÉTODOS: Foi selecionado o município de São Sebastião, localizado no litoral Sudeste do Brasil. Foram utilizados os dados do "Programa de Controle de Vetores de Dengue e Febre Amarela no Estado de São Paulo" que realiza a vigilância entomológica em pontos estratégicos, armadilhas e delimitação de focos. Os pontos estratégicos são imóveis onde existem recipientes em condições favoráveis à proliferação de larvas. Para análise dos dados, foram utilizados os testes de significância estatística: Kruskal-Wallis, Dwass-Steel-Chritchlow-Fligne e Mann-Whitney. RESULTADOS: Verificou-se crescimento anual da positividade de armadilhas e pontos estratégicos para Ae. aegypti e diminuição para Ae. albopictus. Observou-se aumento do número de imaturos de Ae. aegypti e diminuição da outra espécie. Na positividade de imóveis para a presença de larvas, verificou-se aumento gradativo do número de imóveis com Ae. Aegypti, superando a positividade para Ae. albopictus. Houve uma fraca correlação das espécies com os fatores abióticos. As maiores freqüências de imaturos de ambas espécies foram em recipientes artificiais passíveis de remoção. CONCLUSÕES: Os resultados evidenciaram no período de estudo a predominância de Ae. aegypti sobre Ae. albopictus em área urbana, indicando que o crescimento populacional do primeiro parece ter afetado a chance de sua coexistência. Sugere-se que algum processo de seleção natural possa estar operando e desse modo contribuindo para levar à separação das duas espécies.OBJECTIVE: To assess infestation of Aedes aegypti and Aedes albopiticus and describe their association with weather conditions and container colonization in urban areas. METHODS: The town of São Sebastião in the southeastern coast of Brazil

  2. Avaliação da influência da temperatura sobre o desenvolvimento de Aedes albopictus Evaluation of the temperature influence on the development of Aedes albopictus

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    Daniéla Cristina Calado

    2002-04-01

    Full Text Available OBJETIVO: Verificar a influência da temperatura sobre os estágios de desenvolvimento de Aedes albopictus sob condições de laboratório. MÉTODOS: Foi avaliado o período de desenvolvimento dos estágios de ovo, larva e pupa em quatro temperaturas constantes de 15ºC, 20ºC, 25ºC e 30ºC em câmaras climatizadas com umidade relativa do ar oscilando entre 70% e 85% e fotoperíodo de CE 12:12 horas. Pela análise de variância, foram testados os efeitos das quatro temperaturas constantes. RESULTADOS: O período de incubação dos ovos compreendeu 38,38; 19,09; 13,10; e 10,44 dias; o período larval dos indivíduos machos atingiu 30,13; 13,83; 7,36; e 5,57 dias; o período larval para fêmeas apresentou duração de 33,22; 15,00; 8,06; e 6,16 dias; o período pupal em machos compreendeu 8,01; 4,92; 2,40; e 1,76 dias, enquanto nas fêmeas foi de 8,15; 5,11; 2,60; e 1,88 dias sob as temperaturas de 15ºC, 20ºC, 25ºC e 30ºC, respectivamente. Em todos os estágios de desenvolvimento, foram encontradas diferenças significativas, entre as temperaturas avaliadas, em torno de 5% no tempo de desenvolvimento. O período de desenvolvimento foi inversamente relacionado à temperatura, com o período larval mais breve em machos que em fêmeas. Nas temperaturas de 20ºC a 30ºC, o desenvolvimento foi mais breve e com maior viabilidade que a 15ºC. CONCLUSÕES: Nas condições em que foram realizados os experimentos, a temperatura afetou de forma significativa e inversamente proporcional os estágios imaturos de Aedes albopictus. Esses resultados mostram que os estágios imaturos são mais suscetíveis durante os períodos de baixas temperaturas devido ao maior tempo necessário para completar o desenvolvimento, informação que pode ser utilizada em estratégias de controle.OBJECTIVE: To verify the temperature's influence on the Aedes albopictus development stages under laboratory conditions. METHODS: The development period of eggs, larvae and pupas were

  3. Avaliação da influência da temperatura sobre o desenvolvimento de Aedes albopictus

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    Daniéla Cristina Calado

    2002-04-01

    Full Text Available OBJETIVO: Verificar a influência da temperatura sobre os estágios de desenvolvimento de Aedes albopictus sob condições de laboratório. MÉTODOS: Foi avaliado o período de desenvolvimento dos estágios de ovo, larva e pupa em quatro temperaturas constantes de 15ºC, 20ºC, 25ºC e 30ºC em câmaras climatizadas com umidade relativa do ar oscilando entre 70% e 85% e fotoperíodo de CE 12:12 horas. Pela análise de variância, foram testados os efeitos das quatro temperaturas constantes. RESULTADOS: O período de incubação dos ovos compreendeu 38,38; 19,09; 13,10; e 10,44 dias; o período larval dos indivíduos machos atingiu 30,13; 13,83; 7,36; e 5,57 dias; o período larval para fêmeas apresentou duração de 33,22; 15,00; 8,06; e 6,16 dias; o período pupal em machos compreendeu 8,01; 4,92; 2,40; e 1,76 dias, enquanto nas fêmeas foi de 8,15; 5,11; 2,60; e 1,88 dias sob as temperaturas de 15ºC, 20ºC, 25ºC e 30ºC, respectivamente. Em todos os estágios de desenvolvimento, foram encontradas diferenças significativas, entre as temperaturas avaliadas, em torno de 5% no tempo de desenvolvimento. O período de desenvolvimento foi inversamente relacionado à temperatura, com o período larval mais breve em machos que em fêmeas. Nas temperaturas de 20ºC a 30ºC, o desenvolvimento foi mais breve e com maior viabilidade que a 15ºC. CONCLUSÕES: Nas condições em que foram realizados os experimentos, a temperatura afetou de forma significativa e inversamente proporcional os estágios imaturos de Aedes albopictus. Esses resultados mostram que os estágios imaturos são mais suscetíveis durante os períodos de baixas temperaturas devido ao maior tempo necessário para completar o desenvolvimento, informação que pode ser utilizada em estratégias de controle.

  4. Novedades sobre el género Pilosella Hill.: (Asteraceae, Lactuceae) en España, II

    OpenAIRE

    Mateo Sanz, Gonzalo

    2016-01-01

    Se comunica la presencia de diversas especies nuevas del género Pilosella Hill. (Asteraceae, Lactuceae) en España. Novelties on the genus Pilosella Hill (Asteraceae, Lactuceae) in Spain, II. Several species of Pilosella Hill (Asteraceae, Lactuceae) found in Spain.

  5. Efeito larvicida de óleos essenciais de plantas medicinais sobre larvas de Aedes aegypti L. (Diptera:Culicidae

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    Toshik Iarley da Silva

    2017-06-01

    Full Text Available O Aedes aegypti L. é o mosquito mais relevante em termos de inseto de importância para a saúde pública no mundo, pois é transmissor de vírus que causam várias doenças, dentre elas a dengue. Na procura de larvicidas alternativos, os óleos essenciais extraídos de plantas medicinais têm demonstrado alta eficiência. Com isso, objetivou-se com avaliar o potencial larvicida de óleos essenciais, extraídos de espécies medicinais, sobre larvas de A. aegypti. O experimento foi realizado nos Laboratórios de Tecnologia de Produtos e de Entomologia Agrícola da Universidade Federal do Cariri (UFCA, na cidade de Crato, Ceará. Os óleos essenciais de alfazema (Hyptis suaveolens; gonçalo-alves (Astronium fraxinifolium0; alecrim de tabuleiro (Lippia Microphylla; mussambê (Cleome spinosa; marmeleiro (Croton sonderianus; aroeira (Myracrodruon urundeuva; velame (Croton heliotropiifolius e candeeiro (Vanillosmopsis arborea foram extraídos pelo método de hidrodestilação em aparelho tipo Clevenger. Os óleos foram emulsionados com Dimetilsulfóxido 2% (DMSO e diluídos para a concentração de 100 ppm (partes por milhão, utilizando-se o delineamento inteiramente casualisado com nove tratamentos (consistindo nas soluções de 100 ppm de cada óleo mais a solução controle com água e DMSO e quatro repetições, utilizando-se dez larvas para cada tratamento, sendo avaliado o número de larvas mortas. Observou-se que todos os óleos essenciais apresentaram efeito larvicida, porém os de candeeiro e de alfazema foram os que se destacaram.Abstract: Aedes aegypti L. is the most relevant mosquito in terms of public health importance of the world, because it’s the virus transmitter that causes many diseases, among this dengue. Looking for alternatives larvicides, the essential oils extracted from medicinal plants have demonstrated high efficiency. Thereby, it was objectified in this work to evaluate the larvicide potential of some essential oils

  6. GINCANA SOBRE O MOSQUITO Aedes aegypti COM ALUNOS DO ENSINO FUNDAMENTAL DE UMA ESCOLA PÚBLICA DO MUNICÍPIO DE ALEGRE-ES

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    Paola Cerbino Doblas

    2017-02-01

    Full Text Available Os vírus da Dengue, Zica e Chikungunya tem alarmado o Governo e a população tendo em vista o elevado número de casos no Brasil e seus consequentes prejuízos à saúde pública. Dessa forma a escola tem um papel fundamental em instruir seus alunos sobre os riscos, métodos de transmissão e prevenção quanto ao vetor dessas doenças: o mosquito Aedes aegypti. Com o objetivo de prevenir a proliferação do mosquito na cidade de Alegre, ES, os alunos participantes do Programa Institucional de Bolsa de Iniciação à Docência (PIBID de biologia, da Universidade Federal do Espírito Santo (UFES no campus de Alegre, aplicaram uma gincana para alunos do ensino fundamental de uma escola da rede pública do município de Alegre. O resultado da gincana foi benéfico visto que os alunos se demonstraram bastante participativos e mostraram obter conhecimento sobre o assunto. Por meio da análise dos resultados é possível perceber que os diferentes métodos de ensino podem ser muito eficientes para o processo de ensino-aprendizagem.

  7. Evaluación larvicida de suspensiones acuosas de Annona muricata Linnaeus «guanábana» sobre Aedes aegypti Linnaeus (Diptera, Culicidae

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    Miguel Bobadilla

    2013-05-01

    Full Text Available EL presente trabajo evaluó la toxicidad larvicida de suspensiones acuosas provenientes de extractos etanólicos de las semillas, flores, hojas, corteza de ramas y corteza de raíces de Annona muricata L. «guanábana» sobre larvas del IV estadio de Aedes aegypti para determinar de esta manera los niveles de susceptibilidad. El mayor efecto tóxico correspondió a la suspensión de las semillas con un 100% de mortalidad a las 24 horas a 0,5 mg/mL, seguidos por las flores a las 48 horas a 10 mg/mL y hojas a las 36 horas a 100 mg/mL. En semillas, las concentraciones letales al 50% (CL50 y 90% (CL90 a las 48 horas de exposición fueron 0,02 mg/mL y 0,11 mg/mL, en flores 3,33 y 12,16 mg/mL, en hojas 8,25 y 26,87 mg/mL y en corteza de ramas 19,21 y 97,23 mg/mL, respectivamente. Los resultados de las rectas probit-logarítmicas indicaron susceptibilidad de los individuos a cada suspensión, gracias a la acción de diversos principios activos distribuidos en todo el árbol.

  8. Evaluación de un módulo escolar sobre dengue y Aedes aegypti dirigido a escolares en Honduras Evaluation of an educational module on dengue and Aedes aegypti for schoolchildren in Honduras

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    Gustavo Adolfo Ávila Montes

    2004-08-01

    Full Text Available OBJETIVOS: Evaluar la eficacia de un curso sobre salud ambiental y dengue, impartido a alumnos de primaria y encaminado a promover en las madres de familia cambios de comportamiento que lleven a un manejo seguro del agua, a la disposición adecuada de la basura y al control de los criaderos domésticos de Aedes aegypti, el mosquito vector de la enfermedad. MÉTODOS: En el período de abril a noviembre de 2002 se llevó a cabo un ensayo de campo comunitario que incluyó cuatro escuelas públicas de la ciudad de Comayaguela, Honduras, dos de las cuales formaron el grupo de intervención (GI y las otras dos el grupo testigo o de control (GC. Las actividades de recolección de información pre y posintervención incluyeron pruebas de conocimiento a escolares y maestros, encuestas de conocimientos, actitudes y prácticas a madres de familia, encuestas entomológicas (registro sistemático para encontrar depósitos que contengan agua, en busca de larvas de mosquitos en las viviendas de los escolares, entrevistas a profundidad a madres de familia, observaciones participativas en las aulas y un grupo focal posintervención con los maestros. RESULTADOS: Durante el período de la intervención se presentó la mayor epidemia de dengue clásico en la historia de la ciudad capital, que motivó actividades de control a gran escala por parte de la Secretaría de Salud. En el ámbito escolar, el GI mostró un incremento significativo en el conocimiento de los alumnos en las siguientes variables: dengue causado por un virus (78,5% frente a 56,6%, ciclo de vida del vector (79,4% frente a 23,6% y reducción de fuentes -la medida más eficaz de control del vector- (85,2% frente a 58,1%; los incrementos en todas las variables tuvieron un valor P OBJECTIVE: To evaluate the effectiveness of a special course on environmental health and dengue, given to primary school students and intended to promote behavioral changes in the mothers of those students that would lead to

  9. Impact of environmental temperatures on resistance to organophosphate insecticides in Aedes aegypti from Trinidad Repercusión de las temperaturas ambientales sobre la resistencia de Aedes aegypti a los insecticidas organofosforados en Trinidad

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    Karen A. Polson

    2012-07-01

    Full Text Available OBJECTIVE: To examine the effects of increasing larval rearing temperatures on the resistance status of Trinidadian populations of Aedes aegypti to organophosphate (OP insecticides. METHODS: In 2007-2008, bioassays and biochemical assays were conducted on A. aegypti larvae collected in 2006 from eight geographically distinct areas in Trinidad (Trinidad and Tobago. Larval populations were reared at four temperatures (28 ± 2ºC, 32ºC, 34ºC, and 36ºC prior to bioassays with OP insecticides (fenthion, malathion, and temephos and biochemical assays for esterase enzymes. RESULTS: Most larval populations reared at 28 ± 2ºC were susceptible to fenthion (>98% mortality but resistant to malathion and temephos (OBJETIVO: Examinar los efectos del aumento de las temperaturas de desarrollo larvario sobre el estado de resistencia a los insecticidas organofosforados de las poblaciones de Aedes aegypti en Trinidad. MÉTODOS: En 2007 y 2008 se llevaron a cabo ensayos biológicos y bioquímicos en larvas de A. aegypti recogidas en el 2006 de ocho áreas geográficamente separadas en Trinidad (Trinidad y Tabago. Las poblaciones larvarias se desarrollaron en cuatro temperaturas (28 ± 2 ºC, 32 ºC, 34 ºC y 36 ºC antes de los ensayos biológicos con insecticidas organofosforados (fentión, malatión y temefós y los análisis bioquímicos para las enzimas de esterasa. RESULTADOS: La mayoría de las poblaciones larvarias que se desarrollaron a 28 ± 2 ºC fueron susceptibles al fentión (mortalidad > 98% pero resistentes al malatión y al temefós (mortalidad < 80%. Se encontró una asociación positiva entre la resistencia a los insecticidas organofosforados y la mayor actividad de αy β-esterasas en las poblaciones larvarias que se desarrollaron a 28 ± 2 ºC. Aunque las poblaciones larvarias que se desarrollaron a temperaturas mayores mostraron variaciones en la resistencia a los organofosforados, hubo un aumento general de la sensibilidad. Sin embargo

  10. Evaluación del efecto tóxico de extractos de Eupatorium microphyllum L.F. (Asteraceae) sobre larvas de Aedes aegypti (Diptera: Culicidae) en condiciones de laboratorio

    OpenAIRE

    Bello, Felio J.; Rozo, Álvaro; Zapata, Cristina

    2010-01-01

    En el presente trabajo se evaluó la actividad tóxica de extractos de Eupatorium microphyllum L.F. sobre larvas de IV estadio del mosquito Aedes aegypti (Linneaus), bajo condiciones de laboratorio. Se utilizaron extractos acuosos en concentraciones del 500 mg L-1, 1.500 mg L-1 y 2.500 mg L-1 y acetónicos en concentraciones de 10 mg L-1, 20 mg L-1, 30 mg L-1, 40 mg L-1 y 50 mg L-1. Los bioensayos se realizaron por triplicado, cada uno con 20 larvas, expuestas durante 24 horas a 150 mL de soluci...

  11. Sobre a noção de causalidade na medicina: aproximando Austin Bradford Hill e John L. MackieOn the notion of causality in medicine: addressing Austin Bradford Hill and John L. Mackie

    OpenAIRE

    Araújo, Luís Fernando S. C. de; Dalgalarrondo, Paulo; Banzato, Cláudio E. M.

    2014-01-01

    Almost 50 years ago appeared the seminal article by Austin Bradford Hill where he presented parameters for inferring causes from statistical associations, which became known as Hill’s causal criteria. This was a milestone for the renewal of the idea of cause in medicine. Our article revisits his contribution in light of the ideas from the Australian philosopher John L. Mackie, whose important works on causality reached an audience distinct from Hill’s. We suggest that both the Bri...

  12. Influencia de factores abióticos sobre la incidencia de Aedes aegypti en el municipio 10 de Octubre de Ciudad de La Habana, 1982-1992

    OpenAIRE

    MARQUETTI, MARIA DEL CARMEN; CARUS, FERNANDO; AGUILERA, LUCITA; NAVARRO, AGUSTIN

    1995-01-01

    Se realiza un análisis de la relación entre el número de focos de Aedes aegypti, las precipitaciones y las temperaturas ocurridas en las épocas de lluvia y seca durante los años 1982 a 1992, en el municipio 10 de Octubre, en Ciudad de La Habana. No se encontró correlación entre el número de focos y las precipitaciones ocurridas en ambas estaciones, así como tampoco entre el número de focos y las temperaturas, por lo que se enfatiza la necesidad de mantener una estricta vigilancia durante todo...

  13. Parks of Chapel Hill

    Data.gov (United States)

    Town of Chapel Hill, North Carolina — Hours, location, and amenity information for Chapel Hill parks as shown on the Town of Chapel Hill's website. Includes a map with points for each park location.

  14. Fauna de mosquitos asociada con Aedes aegypti en Guaduas, Colombia

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    Víctor Alberto Olano

    1993-06-01

    Full Text Available Durante un estudio sobre la ecología del Aedes aegypti llevado a cabo en el área urbana de Guaduas, Colombia, se hallaron un total de siete especies de mosquitos que compartían hábitats con esta especie. Los criaderos en los cuales se encontró un mayor número de mosquitos asociados con el Aedes aegypti fueron los tanques bajos (albercas. Larvas de Toxorhynchites spp. se encontraron en tanques elevados. Se discute la importancia de estos hallazgos con relación a los aspectos de ecología y control del Aedes aegypti.

  15. Influência do período de quiescência dos ovos sobre o ciclo de vida de Aedes aegypti (Linnaeus, 1762 (Diptera, Culicidae em condições de laboratório

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    Silva Heloisa Helena Garcia da

    1999-01-01

    Full Text Available Estudou-se a influência do período de quiescência dos ovos no ciclo de vida de Aedes aegypti (Linnaeus, 1762 (Diptera, Culicidae em condições de laboratório, na busca de informações que possam melhorar o direcionamento das ações de controle, pois sabe-se que o ovo é a forma mais resistente do ciclo biológico, possibilitando ao mosquito ampla sobrevida, devido à resistência às adversidades climáticas. Os experimentos foram realizados numa câmara biológica, mantida à temperatura de 28 ± 1oC, com umidade relativa de 80 ± 5% e fotofase de 12 horas. Apresentam-se os dados da influência de diferentes períodos de quiescência sobre a eclosão das larvas, desenvolvimento larval e pupal, ciclo evolutivo. Verificou-se o efeito altamente significativo do período de quiescência na eclosão das larvas. O período de quiescência não influenciou nas durações dos períodos de incubação, larval e pupal. Constatou-se que ovos de um mesmo período de quiescência apresentaram períodos de incubação estatisticamente diferentes entre si. As larvas eclodiam em grupos, definidos pela incubação, e este efeito de grupo foi significativo na duração do ciclo. Pode-se afirmar que, em 99,8% dos ciclos, a variação foi determinada pela incubação.

  16. Loess Hills of Iowa

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — This coverage outlines the boundary of the Loess Hills in Iowa at 1:100,000 scale. Criteria applied to the delineation of the Loess Hills included drainage density,...

  17. Comparative study of the inhibition produced by CMU (3-p-chlorophenyl-1. 1-dimethyl-urea) in barley leaves, on the photophosphorilation, photocarboxilation and hill reaction; Estudio comparativo de la inhibicion que produce el CMU sobre la fotogosforilacion, totocarboxilacion y reaccion de hill en cebada. (Hordeum vulgare L.)

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez, J; Sancho, C

    1977-07-01

    The effect of different concentrations of CMU (from 10 {sup -}8 M to 10{sup -}3 H) on the photophosphorilation, photocarboxilation and Hill reaction was studied. CMU (Carbon-H labelled) was utilized to determine the concentration of CMU in leaf parenchyma. Photocarboxilation and photophosphorolation was sensible to concentrations less than 10{sup -}7 M. Hill reaction in isolated chloroplasts was sensible from concentrations of the order of 10{sup -}8 M. (Author) 50 refs.

  18. IDENTIFIKASI AEDES AEGYPTI DAN AEDES ALBOPICTUS

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    Diah Fitri Rahayu

    2013-09-01

    Full Text Available ABSTRAK. Demam Berdarah Dengue (DBD adalah penyakit yang disebabkan oleh virus Dengue yangditularkan oleh nyamuk . Kabupaten Banjarnegara merupakan daerah endemis reseptif DBD. Dari tahun ketahun kasus DBD cenderung meningkat, terutama di tahun 2009 - 2010. Vektor DBD di Kabupaten Banjarnegaraadalah Ae. aegypti dan Ae. albopictus. Ae. aegypti secara makroskopis terlihat sama seperti Ae. albopictus,namun perbedaannya terletak pada morfologi kepala (mesonotum di mana Ae. aegypti memiliki gambar garisseperti kepala kecapi berbentuk dengan dua garis lengkung dan dua garis lurus putih sementara Ae. albopictushanya memiliki satu garis putih di mesonotum tersebut.Kata kunci: identifikasi, Ae. aegypti, Ae. albopictusABSTRACT. Dengue hemorrhagic fever (DHF is a disease caused by the dengue virus transmitted by mosquito.Banjarnegara district is a Dengue receptive endemic area. From year to year the Dengue cases is likely toincrease, especially in 2009 - 2010. The main vector of dengue in Banjarnegara district is Aedes aegypti andAedes albopictus. Ae. aegypti morphologically look like with Ae. albopictus, but the difference lies in the headmorphology (mesonotum where A. aegypti has a picture of the line like a lyre-shaped head with two curved linesand two white straight line while A. albopictus has only one white stripe on the mesonotum.Key words:identification, Ae. aegypti, Ae. albopictus

  19. Hill, Prof. Archibald Vivian

    Indian Academy of Sciences (India)

    Home; Fellowship. Fellow Profile. Elected: 1935 Honorary. Hill, Prof. Archibald Vivian Nobel Laureate (Medicine) - 1922. Date of birth: 26 September 1886. Date of death: 3 June 1977. YouTube; Twitter; Facebook; Blog. Academy News. IAS Logo. 29th Mid-year meeting. Posted on 19 January 2018. The 29th Mid-year ...

  20. Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae against Aedes aegypti (Diptera, Culicidae Atividade larvicida das frações do óleo-resina da planta medicinal brasileira Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae sobre o Aedes aegypti (Diptera, Culicidae

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    Heloísa Helena Garcia da Silva

    2007-06-01

    Full Text Available Oil-resin fractions from Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae were evaluated for larvicidal activity on third larval instars of Aedes aegypti, in searching for alternative control methods for this mosquito. The bioactive fractions were chemically monitored by thin-layer chromatography, ¹H and 13C nuclear magnetic resonance and mass spectrometry. Bioassays were performed using five repetitions, at a temperature of 28 ± 1°C, relative humidity of 80 ± 5% and light and dark cycles of 12h. Mortality was indicated by darkening of the cephalic capsule after 24h of exposure of the larvae to the solutions. The most active fractions were CRM1-4 (sesquiterpenes and CRM5-7 (labdane diterpenes, which showed LC50 values of 0.2 and 0.8ppm, respectively.A atividade larvicida das frações do óleo-resina de Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae foi avaliada em larvas de 3º estádio de Aedes aegypti, na busca de alternativas para o controle desse mosquito. As frações bioativas foram monitoradas quimicamente através de cromatografia de camada delgada, analisada por ressonância magnética nuclear de hidrogênio (¹H e 13C e espectrometria de massas. Os bioensaios foram realizados à temperatura de 28±1°C, 80±5% de umidade relativa e fotofase de 12h, com cinco repetições. A mortalidade foi determinada através do escurecimento da cápsula cefálica, após 24h de exposição das larvas às soluções. As frações mais ativas foram CRM1-4 (sesquiterpenos e CRM5-7 (diterpeno labdano, que mostraram os valores de CL50 de 0,2 e 0,8ppm, respectivamente.

  1. Systematics of Aedes Mosquito Project.

    Science.gov (United States)

    1984-01-01

    Fever and Zika viruses . During a recent field trip to Cameroon and Kenya in the early part of 1983 numerous specimens were collected, mostly as reared...1942) isolated Yellow Fever virus is Aedes (Stejomyia) broeliae (Theobald) and is the common man-biting member of -th-e complex in East Africa. The...PERIOD COVERED Five Month Report Systematics of Aedes Mosquito Project August 1 - December 31, 1983 p - 6. PERFORMING ORG. REPORT NUMBER 7. AUTHOR(&) S

  2. Aedes albopictus em área rural do Brasil e implicações na transmissão de febre amarela silvestre Aedes albopictus in rural zone of Brazil and its implication in the sylvatic yellow fever transmission

    Directory of Open Access Journals (Sweden)

    Almério de Castro Gomes

    1999-02-01

    Full Text Available Durante estudos ecológicos sobre mosquitos anofelíneos no município de Bataguassu, Estado de Mato Grosso do Sul, foram encontradas larvas e adultos de Aedes albopictus. Pela primeira vez sua introdução ocorre numa área enzoótica do vírus selvático da febre amarela no Brasil. Isto sugere risco potencial para transferência desse vírus para área urbana infestada com Aedes aegypti.Larvae and adult forms of Aedes albopictus were found during ecological study of anopheline mosquitos in the rural zone of the state of Mato Grosso do Sul in Brazil. This occurrence was registered, for the first time in Brazil, in an enzoootic area if sylvatic yellow fever virus. This implies a potential risk of the transfer of this virus to an urban area infested with Aedes aegypti.

  3. Registro de altitud del Aedes Aegypti en Colombia

    Directory of Open Access Journals (Sweden)

    Marco F. Suárez

    1981-12-01

    Full Text Available En Colombia se había considerado que el mosquito Aedes oegypti no habitaba en alturas superiores a los 1.500 metros sobre el nivel del mar (m/nm. Se disponía de los registros de Oiba [Santander, a 1.400 m/nm  y con temperatura media de 20°C y del Líbano (Tolima], a 1.585 m/nm y con temperatura media de 20°C. (1.

  4. Rhamnolipids: solution against Aedes aegypti?

    Directory of Open Access Journals (Sweden)

    Vinicius Luiz Silva

    2015-02-01

    Full Text Available Aedes aegypti mosquitoes are the primary transmitters of dengue fever, urban yellow fever and chikungunya viruses. This mosquito has developed resistance to the insecticides currently used to control their populations. These chemical insecticides are harmful to the environment and can have negative effects on human health. Rhamnolipids are environmentally compatible biological surfactants, but their insecticidal activity has not been extensively studied. The present study evaluated the potential larvicidal, insecticidal and repellent activities of rhamnolipids against Aedes aegypti. At concentrations of 800, 900 and 1000 mg/L, rhamnolipids eliminated all mosquito larvae in 18 hours and killed 100% of adults at 1000 mg/L. According to the results it may be conclude that rhamnolipids should be applied to control larvae and mosquitos besides present the repellency activity against Aedes aegypti.

  5. Programa de computador para reconhecimento da larva de Aedes aegypti e Aedes albopictus

    OpenAIRE

    São Thiago, André Iwersen de; Kupek, Emil; Ferreira Neto, Joaquim Alves; São Thiago, Paulo de Tarso

    2002-01-01

    Software for pattern recognition of the larvae of mosquitoes Aedes aegypti and Aedes albopictus, biological vectors of dengue and yellow fever, has been developed. Rapid field identification of larva using a digital camera linked to a laptop computer equipped with this software may greatly help prevention campaigns.Foi desenvolvido um programa de computador para reconhecimento da larva de Aedes aegypti e Aedes albopictus, vetores biológicos de dengue e febre amarela. O programa possibilita rá...

  6. Antigravity hills are visual illusions.

    Science.gov (United States)

    Bressan, Paola; Garlaschelli, Luigi; Barracano, Monica

    2003-09-01

    Antigravity hills, also known as spook hills or magnetic hills, are natural places where cars put into neutral are seen to move uphill on a slightly sloping road, apparently defying the law of gravity. We show that these effects, popularly attributed to gravitational anomalies, are in fact visual illusions. We re-created all the known types of antigravity spots in our laboratory using tabletop models; the number of visible stretches of road, their slant, and the height of the visible horizon were systematically varied in four experiments. We conclude that antigravity-hill effects follow from a misperception of the eye level relative to gravity, caused by the presence of either contextual inclines or a false horizon line.

  7. Hill's formula

    Energy Technology Data Exchange (ETDEWEB)

    Bolotin, Sergey V [Steklov Mathematical Institute, Russian Academy of Sciences, Moscow (Russian Federation); Treschev, Dmitrii V [M. V. Lomonosov Moscow State University, Moscow (Russian Federation)

    2010-07-27

    In his study of periodic orbits of the three-body problem, Hill obtained a formula connecting the characteristic polynomial of the monodromy matrix of a periodic orbit with the infinite determinant of the Hessian of the action functional. A mathematically rigorous definition of the Hill determinant and a proof of Hill's formula were obtained later by Poincare. Here two multidimensional generalizations of Hill's formula are given: for discrete Lagrangian systems (symplectic twist maps) and for continuous Lagrangian systems. Additional aspects appearing in the presence of symmetries or reversibility are discussed. Also studied is the change of the Morse index of a periodic trajectory upon reduction of order in a system with symmetries. Applications are given to the problem of stability of periodic orbits. Bibliography: 34 titles.

  8. Are hills like white elephants?

    Directory of Open Access Journals (Sweden)

    Sunil Sharma

    2016-11-01

    Full Text Available 'Are Hills Like White Elephants?' is, of course, inspired by Hemingway; the tribute reflects on the abiding relevance of serious art in a changed world and extends the boundaries of his message to other human situations.

  9. Truck-mounted Area-wide Application of Pyriproxyfen Targeting Aedes aegypti and Aedes albopictus in Northeast Florida

    Science.gov (United States)

    2014-12-01

    TRUCK-MOUNTED AREA-WIDE APPLICATION OF PYRIPROXYFEN TARGETING AEDES AEGYPTI AND AEDES ALBOPICTUS IN NORTHEAST FLORIDA1 CARL W. DOUD,2,3 ANTHONY M...truck-mounted ultra-low volume applications of pyriproxyfen against Aedes aegypti larvae in artificial water containers and wild adult Ae. albopictus...larval control, Aedes aegypti, Aedes albopictus INTRODUCTION Aedes albopictus (Skuse) (Asian tiger mosquito) and Ae. aegypti (L.) (yellow fever

  10. Chikungunya Virus Infection of Aedes Mosquitoes.

    Science.gov (United States)

    Wong, Hui Vern; Chan, Yoke Fun; Sam, I-Ching; Sulaiman, Wan Yusof Wan; Vythilingam, Indra

    2016-01-01

    In vivo infection of mosquitoes is an important method to study and characterize arthropod-borne viruses. Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that is transmitted primarily by Aedes mosquitoes. In this chapter, we describe a protocol for infection of CHIKV in two species of Aedes mosquitoes, Aedes aegypti and Aedes albopictus, together with the isolation of CHIKV in different parts of the infected mosquito such as midgut, legs, wings, salivary gland, head, and saliva. This allows the study of viral infection, replication and dissemination within the mosquito vector.

  11. Recomendaciones para la vigilancia de Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Roberto Barrera

    2016-09-01

    Full Text Available La enfermedades causadas por arbovirus transmitidos por Aedes aegypti, como el dengue, el chikungunya y el zika, continúan aumentando en incidencia anual y expansión geográfica. Una limitación clave para el control de A. aegypti ha sido la ausencia de herramientas eficaces para vigilar su población y poder determinar las medidas de control que realmente funcionan. La vigilancia de A. aegypti se ha basado principalmente en la obtención de los índices aédicos, los cuales guardan poca relación con el número de hembras del mosquito, que son las responsables de la transmisión de los virus. El reciente desarrollo de técnicas de muestreo de adultos de este vector promete facilitar las labores de vigilancia y control. En esta revisión se presentan las diversas técnicas de vigilancia del mosquito, así como una discusión sobre su utilidad, con recomendaciones para lograr una vigilancia entomológica más efectiva.

  12. Soufriere Hills Volcano

    Science.gov (United States)

    2002-01-01

    In this ASTER image of Soufriere Hills Volcano on Montserrat in the Caribbean, continued eruptive activity is evident by the extensive smoke and ash plume streaming towards the west-southwest. Significant eruptive activity began in 1995, forcing the authorities to evacuate more than 7,000 of the island's original population of 11,000. The primary risk now is to the northern part of the island and to the airport. Small rockfalls and pyroclastic flows (ash, rock and hot gases) are common at this time due to continued growth of the dome at the volcano's summit.This image was acquired on October 29, 2002 by the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) on NASA's Terra satellite. With its 14 spectral bands from the visible to the thermal infrared wavelength region, and its high spatial resolution of 15 to 90 meters (about 50 to 300 feet), ASTER images Earth to map and monitor the changing surface of our planet.ASTER is one of five Earth-observing instruments launched December 18, 1999, on NASA's Terra satellite. The instrument was built by Japan's Ministry of Economy, Trade and Industry. A joint U.S./Japan science team is responsible for validation and calibration of the instrument and the data products.The broad spectral coverage and high spectral resolution of ASTER will provide scientists in numerous disciplines with critical information for surface mapping, and monitoring of dynamic conditions and temporal change. Example applications are: monitoring glacial advances and retreats; monitoring potentially active volcanoes; identifying crop stress; determining cloud morphology and physical properties; wetlands evaluation; thermal pollution monitoring; coral reef degradation; surface temperature mapping of soils and geology; and measuring surface heat balance.Dr. Anne Kahle at NASA's Jet Propulsion Laboratory, Pasadena, California, is the U.S. Science team leader; Bjorn Eng of JPL is the project manager. The Terra mission is part of NASA

  13. Globally invasive, withdrawing at home: Aedes albopictus and Aedes japonicus facing the rise of Aedes flavopictus

    Science.gov (United States)

    Chaves, Luis Fernando

    2016-11-01

    It has been suggested that climate change may have facilitated the global expansion of invasive disease vectors, since several species have expanded their range as temperatures have warmed. Here, we present results from observations on two major global invasive mosquito vectors (Diptera: Culicidae), Aedes albopictus (Skuse) and Aedes japonicus (Theobald), across the altitudinal range of Mt. Konpira, Nagasaki, Japan, a location within their native range, where Aedes flavopictus Yamada, formerly a rare species, has now become dominant. Spatial abundance patterns of the three species suggest that temperature is an important factor influencing their adult distribution across the altitudinal range of Mt. Konpira. Temporal abundance patterns, by contrast, were associated with rainfall and showed signals of density-dependent regulation in the three species. The spatial and temporal analysis of abundance patterns showed that Ae. flavopictus and Ae. albopictus were negatively associated, even when accounting for differential impacts of weather and other environmental factors in their co-occurrence patterns. Our results highlight a contingency in the expansion of invasive vectors, the potential emergence of changes in their interactions with species in their native communities, and raise the question of whether these changes might be useful to predict the emergence of future invasive vectors.

  14. Comparison of BG-Sentinel Trap and Oviposition Cups for Aedes aegypti and Aedes albopictus Surveillance in Jacksonville, Florida, USA

    Science.gov (United States)

    2015-03-01

    COMPARISON OF BG-SENTINELH TRAP AND OVIPOSITION CUPS FOR AEDES AEGYPTI AND AEDES ALBOPICTUS SURVEILLANCE IN JACKSONVILLE, FLORIDA, USA JENNIFER A...trap and oviposition cups (OCs) have both proven effective in the surveillance of Aedes species. This study aimed to determine which of the 2 traps could...best characterize the relative population sizes of Aedes albopictus and Aedes aegypti in an urban section of Jacksonville, FL. Until 1986, Ae

  15. IMPLEMENTASI SANDI HILL UNTUK PENYANDIAN CITRA

    Directory of Open Access Journals (Sweden)

    JJ Siang

    2002-01-01

    Full Text Available Hill's code is one of text encoding technique. In this research, Hill's code is extended to image encoding. The image used is BMP 24 bit format. 2x2 and 3x3 matrices is used as a key. The results show that Hill's code is suitable for image whose RGB values vary highly. On the contrary, it is not suitable for less varied RGB images since its original pattern is still persisted in encrypted image. Hill's code for image encoding has also disadvantage in the case that the key matrix is not unique. However, for daily application, with good key matrix, Hill's code can be applied to encode image since it's process only deals with simple matrix operation so it become fast. Abstract in Bahasa Indonesia : Sandi Hill merupakan salah satu teknik penyandian teks. Dalam penelitian ini, pemakaian sandi Hill diperluas dari teks ke citra bertipe BMP 24 bit. Matriks yang dipakai berordo 2x2 dan 3x3. Hasil percobaan menunjukkan bahwa sandi Hill cocok untuk enkripsi citra dengan variasi nilai RGB antar piksel berdekatan yang tinggi (seperti foto, tapi tidak cocok untuk citra dengan variasi nilai RGB yang rendah (seperti gambar kartun karena pola citra asli masih tampak dalam citra sandi. Sandi Hill juga memiliki kelemahan dalam hal tidak tunggalnya matriks kunci yang dapat dipakai. Akan tetapi untuk pemakaian biasa, dengan pemilihan matriks kunci yang baik, sandi Hill dapat dipakai untuk penyandian karena hanya melibatkan operasi matriks biasa sehingga prosesnya relatif cepat. Kata kunci: Sandi Hill, Citra, Relatif Prima.

  16. Mapping the spatial distribution of Aedes aegypti and Aedes albopictus.

    Science.gov (United States)

    Ding, Fangyu; Fu, Jingying; Jiang, Dong; Hao, Mengmeng; Lin, Gang

    2018-02-01

    Mosquito-borne infectious diseases, such as Rift Valley fever, Dengue, Chikungunya and Zika, have caused mass human death with the transnational expansion fueled by economic globalization. Simulating the distribution of the disease vectors is of great importance in formulating public health planning and disease control strategies. In the present study, we simulated the global distribution of Aedes aegypti and Aedes albopictus at a 5×5km spatial resolution with high-dimensional multidisciplinary datasets and machine learning methods Three relatively popular and robust machine learning models, including support vector machine (SVM), gradient boosting machine (GBM) and random forest (RF), were used. During the fine-tuning process based on training datasets of A. aegypti and A. albopictus, RF models achieved the highest performance with an area under the curve (AUC) of 0.973 and 0.974, respectively, followed by GBM (AUC of 0.971 and 0.972, respectively) and SVM (AUC of 0.963 and 0.964, respectively) models. The simulation difference between RF and GBM models was not statistically significant (p>0.05) based on the validation datasets, whereas statistically significant differences (p<0.05) were observed for RF and GBM simulations compared with SVM simulations. From the simulated maps derived from RF models, we observed that the distribution of A. albopictus was wider than that of A. aegypti along a latitudinal gradient. The discriminatory power of each factor in simulating the global distribution of the two species was also analyzed. Our results provided fundamental information for further study on disease transmission simulation and risk assessment. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. The man and the hill

    Science.gov (United States)

    Leopold, Luna Bergere

    1962-01-01

    He was sitting on a large slab of rock. As he looked at the cloud of dust hanging hazily on the horizon, the piece of antler and the block of flint he held in his hand hung as if they were suspended from their previous rapid motion. The man gazed intently across the swaying grass which rose in wave-like billows across the distant hills. What was that dust - a herd of buffalo, a band of hunters, or were coyotes chasing the antelope again? After watching for a while he started again to chip the flint with a rapid twisting motion of the bone in his right hand. The little chips of flint fell in the grass before him. It is the same hill but the scene has changed. Seated on the same rock, holding the reins of a saddle horse, a man dressed in buckskin took the fur cap off his head and wiped his brow. He was looking intently across a brown and desolate landscape at a cloud of dust on the far horizon. Was it the hostile tribe of Indians? It could be buffalo. Nervously he kicked at the ground with the deerhide moccasin, pushing the flint chips out of the way. He wiped the dust from his long rifle. What a terrible place - no water, practically no grass, everything bare and brown. Now at sunset, slanting across the hills green with springtime, a cowman sits on a big rock, pushes his sombrero back on his head, and looks across the valley at a large but quiet herd of stock, moving slowly as each steer walks from one lush patch of grass to another, nibbling. Suddenly he stood up. Far on the horizon some dark objects were moving. Is it the sheepmen? Could it be the stage coach from Baggs to the Sweetwater Crossing?Same hill - a gray truck was grinding slowly toward the summit. It pulled up near a small fenced enclosure where there were some instruments painted a bright silver color. A man stepped out of the truck and turned to his younger companion, "You've never found an arrowhead? Maybe you have never thought about it correctly. If you want to find where an Indian camped long

  18. Dispersal of Engineered Male Aedes aegypti Mosquitoes.

    Science.gov (United States)

    Winskill, Peter; Carvalho, Danilo O; Capurro, Margareth L; Alphey, Luke; Donnelly, Christl A; McKemey, Andrew R

    2015-11-01

    Aedes aegypti, the principal vector of dengue fever, have been genetically engineered for use in a sterile insect control programme. To improve our understanding of the dispersal ecology of mosquitoes and to inform appropriate release strategies of 'genetically sterile' male Aedes aegypti detailed knowledge of the dispersal ability of the released insects is needed. The dispersal ability of released 'genetically sterile' male Aedes aegypti at a field site in Brazil has been estimated. Dispersal kernels embedded within a generalized linear model framework were used to analyse data collected from three large scale mark release recapture studies. The methodology has been applied to previously published dispersal data to compare the dispersal ability of 'genetically sterile' male Aedes aegypti in contrasting environments. We parameterised dispersal kernels and estimated the mean distance travelled for insects in Brazil: 52.8 m (95% CI: 49.9 m, 56.8 m) and Malaysia: 58.0 m (95% CI: 51.1 m, 71.0 m). Our results provide specific, detailed estimates of the dispersal characteristics of released 'genetically sterile' male Aedes aegypti in the field. The comparative analysis indicates that despite differing environments and recapture rates, key features of the insects' dispersal kernels are conserved across the two studies. The results can be used to inform both risk assessments and release programmes using 'genetically sterile' male Aedes aegypti.

  19. Hill climbing algorithms and trivium

    DEFF Research Database (Denmark)

    Borghoff, Julia; Knudsen, Lars Ramkilde; Matusiewicz, Krystian

    2011-01-01

    This paper proposes a new method to solve certain classes of systems of multivariate equations over the binary field and its cryptanalytical applications. We show how heuristic optimization methods such as hill climbing algorithms can be relevant to solving systems of multivariate equations....... A characteristic of equation systems that may be efficiently solvable by the means of such algorithms is provided. As an example, we investigate equation systems induced by the problem of recovering the internal state of the stream cipher Trivium. We propose an improved variant of the simulated annealing method...

  20. Wolbachia Sebagai Alternatif Pengendalian Vektor Nyamuk Aedes SP.

    OpenAIRE

    Lusiyana, Novyan

    2014-01-01

    Demam Berdarah Dengue (DBD) adalah penyakit yang disebabkan oleh virus dengue. Virus dengue ditransmisikan kepada manusia melalui perantara vektor yaitu nyamuk Aedes aegypti dan Aedes albopictus (Aedes sp.). Penyakit ini dapat terjadi sepanjang tahun dan meningkat seiring dengan datangnya musim penghujan. Penyakit DBD memiliki mortalitas yang cukup tinggi terutama di negara-negara beriklim tropis dimana banyak faktor yang mendukung perkembangbiakan nyamuk vektor tersebut.

  1. Aedes albopictus em área rural do Brasil e implicações na transmissão de febre amarela silvestre

    Directory of Open Access Journals (Sweden)

    Almério de Castro Gomes

    1999-02-01

    Full Text Available Durante estudos ecológicos sobre mosquitos anofelíneos no município de Bataguassu, Estado de Mato Grosso do Sul, foram encontradas larvas e adultos de Aedes albopictus. Pela primeira vez sua introdução ocorre numa área enzoótica do vírus selvático da febre amarela no Brasil. Isto sugere risco potencial para transferência desse vírus para área urbana infestada com Aedes aegypti.

  2. Aedes albopictus em área rural do Brasil e implicações na transmissão de febre amarela silvestre

    Directory of Open Access Journals (Sweden)

    Gomes Almério de Castro

    1999-01-01

    Full Text Available Durante estudos ecológicos sobre mosquitos anofelíneos no município de Bataguassu, Estado de Mato Grosso do Sul, foram encontradas larvas e adultos de Aedes albopictus. Pela primeira vez sua introdução ocorre numa área enzoótica do vírus selvático da febre amarela no Brasil. Isto sugere risco potencial para transferência desse vírus para área urbana infestada com Aedes aegypti.

  3. On the analysis of parasite effect for Aedes aegypti and Aedes albopictus population

    Science.gov (United States)

    Kallista, Meta; Aldila, Dipo; Nuraini, Nuning; Soewono, Edy

    2014-03-01

    It has been reported in some countries that the population of Aedes aegypti has been significantly reduced by the invasion of Aedes albopictus. There has been a hypothesis explaining this phenomenon of which investigated the influence of parasites pathogenesis to the competition between these two mosquito species in the fields. Ascogregarina taiwanensis and Ascogregarina culicis are known as parasites that infect Aedes albopictus and Aedes aegypti, respectively. Several studies have concluded that Ascogregarina taiwanensis caused high fatality for Aedes aegypti larvae, but Ascogregarina culicis was not pathogenic to Aedes albopictus larvae. Therefore, Ascogregarina taiwanensis may contribute to reduce the number of populations Aedes aegypti in the fields. Inspired by these facts, a mathematical model depicting interaction between parasites and mosquitoes is constructed in this paper. In this model are included six dynamic mosquito compartments, i.e. egg, larvae, infected larvae, adult, infected adult and one dynamic compartment for parasite. Derivation of the existence criteria and the stability analysis of parasite-free equilibrium as well as the basic offspring for the model are presented. Numerical simulations for sensitivity analysis indicating the invasive species for variation parameters are shown.

  4. Geographic distribution of Aedes aegypti and Aedes albopictus collected from used tires in Vietnam.

    Science.gov (United States)

    Higa, Yukiko; Yen, Nguyen Thi; Kawada, Hitoshi; Son, Tran Hai; Hoa, Nguyen Thuy; Takagi, Masahiro

    2010-03-01

    The spatial distribution of Aedes aegypti and Aedes albopictus in environmental and geographical zones, e.g., urban-rural, coastal-mountainous, and north-south, was investigated throughout Vietnam. Immature stages were collected from used tires along roads. The effects of regions, seasons, and the degree of urbanization on the density and the frequency were statistically analyzed. Aedes aegypti predominated in the southern and central regions, while Ae. albopictus predominated in the northern region, which may be related to climatic conditions (temperature and rainfall). Larval collection from used tires may be suitable to assess rapidly the current distribution of dengue mosquitoes for estimating health risks and implementing vector control measures.

  5. Cruzamiento interespecífico entre Aedes aegypti y Aedes albopictus en el laboratorio

    OpenAIRE

    Martínez López, Yanisley; Martinez Pérez, Yanet; Acosta Rodríguez, Miriam; Fuentes González, Omar

    2014-01-01

    Introducción: existen algunos estudios realizados para verificar el posible apareamiento interespecífico, pero solo algunos trabajos han obtenido resultados positivos en este fenómeno. Objetivo: probar la posibilidad de obtener huevos viables del cruce entre Aedes aegypti y Aedes albopictus. Métodos: experimentos de apareamiento recíproco entre Aedes aegypti procedentes del insectario del Departamento de Control de Vectores del Instituto de Medicina Tropical "Pedro Kourí" y una población de A...

  6. The Camp Hill Project: Objectives and Design

    Science.gov (United States)

    Mattingly, John B.

    1976-01-01

    Available from: EC 090 474. Outlined are the problems and objectives of Pennsylvania's Camp Hill Project--a program designed to complete psychological needs assessments for juveniles incarcerated at Camp Hill, to develop project policies and guidelines in preparation for meeting with juvenile court judges, and to hire staff. (SBH)

  7. Criadouros de Aedes (Stegomyia aegypti (Linnaeus, 1762 em bromélias nativas na Cidade de Vitória, ES Aedes (Stegomyia aegypti (Linnaeus, 1762 breeding sites in native bromeliads in Vitória City, ES

    Directory of Open Access Journals (Sweden)

    José Benedito Malta Varejão

    2005-05-01

    Full Text Available Alguns insetos transmissores de doenças procriam exclusivamente nas proximidades das residências. O Aedes aegypti, responsável por epidemias de dengue em cidades brasileiras, representa sério risco também para a febre amarela. Com o insucesso da campanha de erradicação do inseto, justifica-se a busca de criadouros fora do alcance das medidas de controle atualmente adotadas. Na Cidade de Vitória, ES, investigou-se a ocorrência de criadouros de Aedes aegypti na água coletada em bromélias nativas, sobre as rochas. Paralelamente, avaliou-se a infestação predial nas áreas urbanas contíguas. Em quatro das cinco áreas investigadas foram encontradas larvas de culicídeos nas bromélias, sendo que em duas foi identificado Aedes aegypti. A presença dos criadouros em bromélias não guardou relação com a infestação predial nas áreas próximas. Torna-se necessário definir se os criadouros em bromélias constituem focos primários do Aedes aegypti, ou se representam uma conseqüência da elevada infestação urbana.Some insects that are vectors of human diseases have accompanied man in his migrations throughout the world and breed exclusively in the proximity of human dwellings. The mosquito Aedes aegypti has been responsible for epidemics of dengue in Brazil and its presence also constitutes a serious risk for future outbreaks of urban yellow fever. The failure of campaigns to eradicate this species justifies the search for alternative breeding sites, which may be beyond the reach of present control measures. In this study the occurrence of Aedes aegypti breeding sites in native bromeliads on rocky slopes was investigated in five areas of Vitória, capital of the Brazilian State of Espírito Santo, ES. Water contained in the bromeliads was collected with the aid of a suction apparatus to search for culicid larvae. The degree of infestation of buildings in adjacent urban areas was evaluated simultaneously. Culicid larvae were found in

  8. Redescription and resurrection from synonymy, of Aedes (Ochlerotatus Rhyacophilus Costa Lima, 1933 Redescrição e revalidação de Aedes (Ochlerotatus rhyacophilus Costa Lima, 1933

    Directory of Open Access Journals (Sweden)

    Maria Anice Mureb Sallum

    1988-03-01

    Full Text Available Aedes (Ochlerotatu rhyacophilus Costa Lima i resurrected from the synonymy with Aedes (Ochlerotatus scapularis (Rondani. Lectotype and paralectotypes are designated Larval, pupal and both sexes of adult stages are redescribed and illustrated. Bionomics include a picture of a brreding place. Diagnostic characters for distinguishing rhyacophilus from other species of the Scapularis Group are provided. Some data about known distribution are presented.Aedes (Ochlerotatus rhyacophilus Costa Lima é retida da sinonímia con Aedes (Ochlerotatus scapularis (Rondani. São designados lectótipo e paralectótipos. As formas larval, pupal e adulta de ambos os sexos são redescritas e acompanhadas de ilustrações representativas desses estádios, além do aspecto de um criadouro natural. Apresentam-se caracteres diagnósticos que permitem separar rhyacophilus das outras espécies do Grupo Scapularis, e alguns dados sobre a distribuição geográfica até agora conhecida.

  9. The Origin of the Columbia Hills

    Science.gov (United States)

    Rice, J. W.

    2005-12-01

    The Columbia Hills form a rugged ridge complex comprised of 7 peaks spanning some 3.5 km in length and reaching a maximum height of 106 m (Husband Hill) above the plains of Spirit's landing site. As of this writing Spirit has been exploring the Columbia Hills for over 440 sols (since sol 156 on June 11, 2004). Thus far the origin of the Columbia Hills has remained elusive despite detailed analysis of numerous rock and soil targets, including outcrops. The chemical differences among the 6 distinct rock classes attest to the lithologic diversity and geologic complexity of the Hills. Origin of the Columbia Hills Several hypothesis have been put forth to explain the origin of the Columbia Hills: Old eroded partially buried impact crater rim(s), central peak, residual intracrater fill material, volcanic construct, wrinkle ridge, delta and or combinations of the above. Observations that support various aspects of these multiple hypotheses will be discussed. Numerous buried craters are observed on the floor of Gusev lending credence to the idea that the Columbia Hills are the remains of an ancient impact crater rim or possibly a central peak. Morphologic evidence of the rim of Thira crater and the Columbia Hills appears to support this hypothesis. The Aeolis region contains numerous craters that contain layered materials in the absence of any major fluvial systems. This could imply that the Columbia Hills are the remnants of a formerly extensive unit(s). Gusev contains many hills scattered across its floor such as Grissom, White, Chaffee and numerous other buttes and mesas that may be remnants of a former extensive intracrater deposit. Another possibility is that the Columbia Hills are composed of volcanic materials (cinder cones and associated ash and lava flows) derived either locally or from Apollinaris Patera located 300 km to the north. Several depressions are located in the Columbia Hills. These features could be calderas but are most likely impact craters. The most

  10. New documentary data from the Geominero Museum (the Spanish Geological Survey, Madrid) on the archaeocyathan collection from the Ermitas Hill of Cordoba; Nuevos datos documentales sobre la coleccion de arqueociatos del Cerro de las Ermitas de Cordoba del Museo Geominero (Instituto Geologico y Minero de Espana)

    Energy Technology Data Exchange (ETDEWEB)

    Perejon, A.; Menendez, S.; Rabano, I.; Moreno-Eiris, E.

    2014-06-01

    The archaeocyathan collection of the Ermitas Hill (Cordoba) of the Geominero Museum in Madrid has recently been revised due to the discovery of some documents related to the study by one of this paper's authors of the professional life of Eduardo Hernandez-Pacheco in the National Natural History Museum archives. This documentation shows that the Ermitas Hill archaeocyathan collection now preserved in the Geominero Museum were sent by Eduardo Hernandez-Pacheco to Lucas Mallada before 1902 to be incorporated in the museum's collections and were then studied by Hernandez Sampelayo in 1933 and 1935. This fact implies that this collection is the oldest Spanish archaeocyathan collection deposited in a Spanish museum, besides clearing up the doubts about its origins and when the material was in deposited in the Geominero Museum. (Author)

  11. Workbook on Identification of Aedes Aegypti Larvae.

    Science.gov (United States)

    Pratt, Harry D.; And Others

    This self-instructional booklet is designed to enable yellow fever control workers to identify the larvae of "Aedes aegypti." The morphological features of mosquito larvae are illustrated in this partially programed text, and the distinguishing features of "A. aegypti" indicated. A glossary is included. (AL)

  12. Soufriere Hills, Montserrat, West Indies

    Science.gov (United States)

    2002-01-01

    Volcanic activity on the West Indian island of Montserrat has remained high for several years-the current activity started in 1995. However, remote sensing of the island has been difficult because of frequent cloud cover. The International Space Station crew flew north of the island on a clear day in early July (July 9, 2001) and recorded a vigorous steam plume emanating from the summit of Soufriere Hills. The image also reveals the extensive volcanic mud flows (lahars) and new deltas built out from the coast from the large amounts of volcanic debris delivered downstream by the rivers draining the mountain. As a small island (only 13 x 8 km), all of Montserrat has been impacted by the eruptions. Sources of Information: Smithsonian Global Volcanism Program Italy's Volcanoes Montserrat Volcano Observatory Digital photograph number ISS002-E-9309 was taken on 9 July 2001 from Space Station Alpha and was provided by the Earth Sciences and Image Analysis Laboratory at Johnson Space Center. Additional images taken by astronauts and cosmonauts can be viewed at the NASA-JSC Gateway to Astronaut Photography of Earth.

  13. 27 CFR 9.190 - Red Hill Douglas County, Oregon.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Red Hill Douglas County... Areas § 9.190 Red Hill Douglas County, Oregon. (a) Name. The name of the viticultural area described in this section is “Red Hill Douglas County, Oregon”. For purposes of part 4 of this chapter, “Red Hill...

  14. Nepenthes ampullaria (Nepenthaceae) Pitchers Are Unattractive to Gravid Aedes aegypti and Aedes albopictus (Diptera: Culicidae).

    Science.gov (United States)

    Chou, Lee Yiung; Dykes, Gary A; Wilson, Robyn F; Clarke, Charles M

    2016-02-01

    Nepenthes pitcher plants are colonized by a variety of specialized arthropods. As Aedes mosquitoes are container breeders, Nepenthes pitchers are a potential candidate oviposition site for vector species, such as Aedes aegypti (L.) and Aedes albopictus (Skuse). However, Aedes spp. are not commonly encountered in Nepenthes pitchers, and the environment inside the pitchers of some species is lethal to them. One exception is Nepenthes ampullaria Jack, whose pitchers are known to be colonized by Ae. albopictus on very rare occasions. Given that Ae. albopictus larvae can survive in N. ampullaria pitcher fluids, we sought to determine why pitcher colonization is rare, testing the hypothesis that gravid Aedes mosquitoes are deterred from ovipositing into container habitats that have similar characteristics to N. ampullaria pitchers. Using plastic ovitraps of different sizes, colors, and with different types of fluids (based on the characteristics of N. ampullaria pitchers), we compared oviposition rates by Aedes mosquitoes in urban and rural areas within the geographical range of N. ampullaria near Kuala Lumpur, Malaysia. Ovitraps that were black and large (>250-ml capacity) accumulated significantly more eggs than ovitraps that were smaller, or green in color. In terms of size and color, small, green ovitraps are analogous to N. ampullaria pitchers, indicating that these pitchers are not particularly attractive to gravid Ae. albopictus. Although Aedes spp. are capable of colonizing N. ampullaria pitchers, the pitchers are relatively unattractive to gravid females and do not represent a significant habitat for larvae of dengue vectors at present. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  15. Repellency of Lantana camara (Verbenaceae) flowers against Aedes mosquitoes.

    Science.gov (United States)

    Dua, V K; Gupta, N C; Pandey, A C; Sharma, V P

    1996-09-01

    The repellent effect of Lantana camara flowers was evaluated against Aedes mosquitoes. Lantana flower extract in coconut oil provided 94.5% protection from Aedes albopictus and Ae. aegypti. The mean protection time was 1.9 h. One application of Lantana flower can provide more than 50% protection up to 4 h against the possible bites of Aedes mosquitoes. No adverse effects of the human volunteers were observed through 3 months after the application.

  16. Bunker Hill Sediment Characterization Study

    Energy Technology Data Exchange (ETDEWEB)

    Neal A. Yancey; Debby F. Bruhn

    2009-12-01

    The long history of mineral extraction in the Coeur d’Alene Basin has left a legacy of heavy metal laden mine tailings that have accumulated along the Coeur d’Alene River and its tributaries (U.S. Environmental Protection Agency, 2001; Barton, 2002). Silver, lead and zinc were the primary metals of economic interest in the area, but the ores contained other elements that have become environmental hazards including zinc, cadmium, lead, arsenic, nickel, and copper. The metals have contaminated the water and sediments of Lake Coeur d’Alene, and continue to be transported downstream to Spokane Washington via the Spokane River. In 1983, the EPA listed the Bunker Hill Mining and Metallurgical Complex on the National Priorities List. Since that time, many of the most contaminated areas have been stabilized or isolated, however metal contaminants continue to migrate through the basin. Designation as a Superfund site causes significant problems for the economically depressed communities in the area. Identification of primary sources of contamination can help set priorities for cleanup and cleanup options, which can include source removal, water treatment or no action depending on knowledge about the mobility of contaminants relative to water flow. The mobility of contaminant mobility under natural or engineered conditions depends on multiple factors including the physical and chemical state (or speciation) of metals and the range of processes, some of which can be seasonal, that cause mobilization of metals. As a result, it is particularly important to understand metal speciation (National Research Council, 2005) and the link between speciation and the rates of metal migration and the impact of natural or engineered variations in flow, biological activity or water chemistry.

  17. Evaluation of Bifenthrin and Acorus calamus Linn. Extract against Aedes aegypti L. and Aedes albopictus (Skuse

    Directory of Open Access Journals (Sweden)

    S Sulaiman

    2008-12-01

    Full Text Available "nBackground: Bifenthrin and Acorus calamus Linn extract were evaluated against dengue vectors in the laboratory."nMethods: Both Bifenthrin and Acorus calamus Linn crude hexane extract were bioassayed against the adults and larval stages of dengue vectors Aedes aegypti L. and Aedes albopictus(Skuse in the laboratory."nResults: The A. calamus crude hexane extract exhibited a larvicidal activity against 4th-instar Ae. aegypti larvae with LC50 and LC90 values of 0.4418 and 11.3935 ppm respectively. The plant crude extract exhibited against Ae. albopictus larvae with a higher LC50 and LC90 values of 21.2555 ppm and 36.1061 ppm, respectively. There was a significant difference on the effect of A. calamus extract on both Aedes spp. Larvae (P< 0.05. However, bifenthrin showed a significant difference on larvicidal effect to that of A. calamus hexane extract on both Aedes spp (P< 0.05. In testing the adulticidal activity, this plant extract exhibited the LC50 and LC90 values of 17.4075 and 252.9458 ppm against Ae .aegypti and a higher LC50 and LC90 values of 43.9952 and 446.1365 ppm respectively on Ae. albopictus. There was no significant difference on the effect of A. calamus extract on both Aedes spp adults (P> 0.05."nConclusion: Bifenthrin however showed a significant difference on both Aedes spp adults (P< 0.05. With the wide availability of A. calamus in Malaysia, it could be utilized for controlling dengue vectors. "n 

  18. Peridomestic Aedes malayensis and Aedes albopictus are capable vectors of arboviruses in cities.

    Science.gov (United States)

    Mendenhall, Ian H; Manuel, Menchie; Moorthy, Mahesh; Lee, Theodore T M; Low, Dolyce H W; Missé, Dorothée; Gubler, Duane J; Ellis, Brett R; Ooi, Eng Eong; Pompon, Julien

    2017-06-01

    Dengue and chikungunya are global re-emerging mosquito-borne diseases. In Singapore, sustained vector control coupled with household improvements reduced domestic mosquito populations for the past 45 years, particularly the primary vector Aedes aegypti. However, while disease incidence was low for the first 30 years following vector control implementation, outbreaks have re-emerged in the past 15 years. Epidemiological observations point to the importance of peridomestic infection in areas not targeted by control programs. We investigated the role of vectors in peri-domestic areas. We carried out entomological surveys to identify the Aedes species present in vegetated sites in highly populated areas and determine whether mosquitoes were present in open-air areas frequented by people. We compared vector competence of Aedes albopictus and Aedes malayensis with Ae. aegypti after oral infection with sympatric dengue serotype 2 and chikungunya viruses. Mosquito saliva was tested for the presence of infectious virus particles as a surrogate for transmission following oral infection. We identified Aedes albopictus and Aedes malayensis throughout Singapore and quantified their presence in forested and opened grassy areas. Both Ae. albopictus and Ae. malayensis can occupy sylvatic niches and were highly susceptible to both arboviruses. A majority of saliva of infected Ae. malayensis contained infectious particles for both viruses. Our study reveals the prevalence of competent vectors in peri-domestic areas, including Ae. malayensis for which we established the vector status. Epidemics can be driven by infection foci, which are epidemiologically enhanced in the context of low herd immunity, selective pressure on arbovirus transmission and the presence of infectious asymptomatic persons, all these conditions being present in Singapore. Learning from Singapore's vector control success that reduced domestic vector populations, but has not sustainably reduced arboviral incidence

  19. Dengue virus transovarial transmission by Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Monica Dwi Hartanti

    2016-02-01

    Full Text Available Dengue is a disease that is caused by dengue virus and transmitted to humans through the bite of infected Aedes mosquitoes, especially Aedes aegypti. The disease is hyper-endemic in Southeast Asia, where a more severe form, dengue hemorrhagic fever (DHF and dengue shock syndrome (DSS, is a major public health concern. The purpose of the present study was to find evidence of dengue virus transovarial transmision in local vectors in Jakarta. Fifteen Aedes larvae were collected in 2009 from two areas in Tebet subdistrict in South Jakarta, namely one area with the highest and one with the lowest DHF prevalence. All mosquitoes were reared inside two cages in the laboratory, eight mosquitoes in one cage and seven mosquitoes in another cage and given only sucrose solution as their food. The results showed that 20% of the mosquitoes were positive for dengue virus. Dengue virus detection with an immunohistochemical method demonstrated the occurrence of transovarial transmission in local DHF vectors in Tebet subdistrict. Transovarial dengue infection in Ae.aegypti larvae appeared to maintain or enhance epidemics. Further research is needed to investigate the relation of dengue virus transovarial transmission with DHF endemicity in Jakarta.

  20. Dengue virus transovarial transmission by Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Monica Dwi Hartanti

    2010-08-01

    Full Text Available Dengue is a disease that is caused by dengue virus and transmitted to humans through the bite of infected Aedes mosquitoes, especially Aedes aegypti. The disease is hyper-endemic in Southeast Asia, where a more severe form, dengue hemorrhagic fever (DHF and dengue shock syndrome (DSS, is a major public health concern. The purpose of the present study was to find evidence of dengue virus transovarial transmision in local vectors in Jakarta. Fifteen Aedes larvae were collected in 2009 from two areas in Tebet subdistrict in South Jakarta, namely one area with the highest and one with the lowest DHF prevalence. All mosquitoes were reared inside two cages in the laboratory, eight mosquitoes in one cage and seven mosquitoes in another cage and given only sucrose solution as their food. The results showed that 20% of the mosquitoes were positive for dengue virus. Dengue virus detection with an immunohistochemical method demonstrated the occurrence of transovarial transmission in local DHF vectors in Tebet subdistrict. Transovarial dengue infection in Ae.aegypti larvae appeared to maintain or enhance epidemics. Further research is needed to investigate the relation of dengue virus transovarial transmission with DHF endemicity in Jakarta.

  1. Newtonian and pseudo-Newtonian Hill problem

    International Nuclear Information System (INIS)

    Steklain, A.F.; Letelier, P.S.

    2006-01-01

    A pseudo-Newtonian Hill problem based on the Paczynski-Wiita pseudo-Newtonian potential that reproduces general relativistic effects is presented and compared with the usual Newtonian Hill problem. Poincare maps, Lyapunov exponents and fractal escape techniques are employed to study bounded and unbounded orbits. In particular we consider the systems composed by Sun, Earth and Moon and composed by the Milky Way, the M2 cluster and a star. We find that some pseudo-Newtonian systems-including the M2 system-are more stable than their Newtonian equivalent

  2. Photovoltaics - 10 years after Cherry Hill

    Science.gov (United States)

    Ralph, E. L.

    The status of R&D programs connected with photovoltaic (PV) systems 10 years after the Cherry Hill workshop on 'Photovoltaic Conversion of Solar Energy for Terrestrial Applications' is assessed. The five categories of research recommended by the Cherry Hill Workshop are listed in a table together with their recommended research budget allocations. The workshop categories include: single-crystal Si cells; poly-Si cells; systems and diagnostics. Categories for thin film CdS/Cu2S and CuInSe2 cells are also included. The roles of government and private utility companies in providing adequate financial support for PV research programs is emphasized.

  3. Characterization of Tolypocladium cylindrosporum (Hypocreales: Ophiocordycipitacea) and its impact against Aedes aegypti and Aedes albopictus eggs at low temperature

    Science.gov (United States)

    We examined the growth characteristics of Tolypocladium cylindrosporum IBT 41712 and its potential to infect eggs of Aedes aegypti Linnaeus and Aedes albopictus (Skuse) mosquitoes at low temperature (15 deg C). When grown on sabouraud dextrose agar supplemented with yeast extract, IBT 41712 formed w...

  4. Impact of Tolypocladium cylindrosporum (Hypocreales: Ophiocordycipitaceae) on eggs of Aedes aegypti and Aedes albopictus at low temperature

    Science.gov (United States)

    We examined the potential of Tolypocladium cylindrosporum IBT 41712 to infect eggs of Aedes aegypti Linnaeus and Aedes albopictus (Skuse) mosquitoes at low temperature (15 deg C). To determine the optimum temperature for the fungus, we cultured the fungus at eight temperatures (4, 12, 15, 21, 28, 33...

  5. Mitigating Zika Risk: Aedes Aegypti and Aedes Albopictus Risk Mapping in Brownsville, TX

    Science.gov (United States)

    The presentation is intended to (1) engage audiences on a “System’s” approach to model Aedes species risks at a community level, (2) highlight US EPA EnviroAtlas ecosystem services and data rich capacity, and (3) present ORD research that is directly support EPA...

  6. The Kapsiki of the Mandara Hills

    NARCIS (Netherlands)

    Beek, van W.E.A.

    1987-01-01

    The Kapsiki of Cameroon and the Higi of Nigeria are two tribes from the Mandara hills area of central and western Africa. Though they form one coherent group of villages, they are usually considered as two separate ethnic units. The author normally uses the term Kapsiki for both. Based on fieldwork

  7. Accounting for imperfect detection in Hill numbers for biodiversity studies

    Science.gov (United States)

    Broms, Kristin M.; Hooten, Mevin B.; Fitzpatrick, Ryan M.

    2015-01-01

    Hill numbers unify biodiversity metrics by combining several into one expression. For example, species richness, Shannon's diversity index and the Gini–Simpson index are a few of the most used diversity measures, and they can be expressed as Hill numbers. Traditionally, Hill numbers have been calculated from relative abundance data, but the expression has been modified to use incidence data as well. We demonstrate an approach for estimating Hill numbers using an occupancy modelling framework that accounts for imperfect detection.

  8. Ecología del Aedes aegypti en un pueblo de Colombia, Suramérica

    Directory of Open Access Journals (Sweden)

    Milton E. Tinker

    1993-03-01

    Full Text Available Un estudio de la ecología de las larvas y adultos del Aedes aegypti fue llevado a cabo en Guaduas (Cundinamarca entre noviembre de 1978 y noviembre de 1979. Este estudio comprendió cuatro ciclos de inspección, dos en época seca y dos en época lluviosa. Las albercas fueron encontradas como el mejor hábitat larvario para ambas estaciones. Las llantas fueron igualmente importantes en la época de lluvia. Las alcobas constituyeron el hábitat más favorable para los adultos. En general más predios estaban infestados por adultos que por larvas. La prueba de las precipitinas indicó que la mayoría de las hembras de Aedes aegypti se alimentaron sobre el hombre. Las colecciones con cebo humano demostraron 2 picos de actividad: 10 -1 1 a.m. y 4-5 p.m.

  9. Ecological interactions in Aedes species on Reunion Island.

    Science.gov (United States)

    Bagny Beilhe, L; Delatte, H; Juliano, S A; Fontenille, D; Quilici, S

    2013-12-01

    Two invasive, container-breeding mosquito species, Aedes aegypti (Stegomyia aegypti) and Aedes albopictus (Stegomyia albopicta) (Diptera: Culicidae), have different distribution patterns on Reunion Island. Aedes albopictus occurs in all areas and Ae. aegypti colonizes only some restricted areas already occupied by Ae. albopictus. This study investigates the abiotic and biotic ecological mechanisms that determine the distribution of Aedes species on Reunion Island. Life history traits (duration of immature stages, survivorship, fecundity, estimated finite rate of increase) in Ae. aegypti and Ae. albopictus were compared at different temperatures. These fitness measures were characterized in both species in response to competitive interactions among larvae. Aedes aegypti was drastically affected by temperature, performing well only at around 25 °C, at which it achieved its highest survivorship and greatest estimated rate of increase. The narrow distribution of this species in the field on Reunion Island may thus relate to its poor ability to cope with unfavourable temperatures. Aedes aegypti was also more negatively affected by high population densities and to some extent by interactions with Ae. albopictus, particularly in the context of limited food supplies. Aedes albopictus exhibited better population performance across a range of environmental conditions. Its ecological plasticity and its superior competitive ability relative to its congener may further enhance its invasion success on Reunion Island. © 2012 The Royal Entomological Society.

  10. La Crosse Encephalitis Virus Infection in Field-Collected Aedes albopictus, Aedes japonicus, and Aedes triseriatus in Tennessee.

    Science.gov (United States)

    Westby, Katie M; Fritzen, Charissa; Paulsen, Dave; Poindexter, Stephanie; Moncayo, Abelardo C

    2015-09-01

    La Crosse virus (LACV) is a mosquito-borne virus and a major cause of pediatric encephalitis in the USA. La Crosse virus emerged in Tennessee and other states in the Appalachian region in 1997. We investigated LACV infection rates and seasonal abundances of the native mosquito vector, Aedes triseriatus, and 2 recently introduced mosquito species, Ae. albopictus and Ae. japonicus, in an emerging disease focus in Tennessee. Mosquitoes were collected using multiple trapping methods specific for Aedes mosquitoes at recent human case sites. Mosquito pools were tested via reverse transcriptase-polymerase chain reaction (RT-PCR) of the S segment to detect multiple Bunyamwera and California serogroup viruses, including LACV, as well as real-time RT-PCR of the M segment. A total of 54 mosquito pools were positive, including wild-caught adult females and laboratory-reared adults, demonstrating transovarial transmission in all 3 species. Maximum likelihood estimates (per 1,000 mosquitoes) were 2.72 for Ae. triseriatus, 3.01 for Ae. albopictus, and 0.63 for Ae. japonicus. We conclude that Ae. triseriatus and Ae. albopictus are important LACV vectors and that Ae. japonicus also may be involved in virus maintenance and transmission.

  11. Risk factors for the presence of Aedes aegypti and Aedes albopictus in domestic water-holding containers in areas impacted by the Nam Theun 2 hydroelectric project, Laos

    NARCIS (Netherlands)

    Hiscox, A.F.; Kaye, A.; Vongphayloth, K.; Banks, I.; Piffer, M.; Khammanithong, P.; Sananikhom, P.; Kaul, S.; Hill, N.; Lindsay, S.W.; Brey, P.T.

    2013-01-01

    We assessed risk factors for vectors of dengue and chikungunya viruses near a new hydroelectric project, Nam Theun 2, in Laos. Immature stages of Aedes aegypti were found only in sites within 40 km of the urban provincial capital, but Aedes albopictus was found throughout. Aedes aegypti pupae were

  12. Efektivitas Ovitrap Bambu terhadap Jumlah Jentik Aedes sp yang Terperangkap

    Directory of Open Access Journals (Sweden)

    Wiwit Aditama

    2015-05-01

    places of 10 subdistricts. Data of trapped Aedes sp larvae numbers was collected four times repeatedly within one week time-lapse. Analysis was conducted using the mean number of larvae in ovitraps and ovitrap index. The number of Aedes sp larvae trapped was 1,265. The most effective ovitrap is piece of bamboo, mean = 123, p value = 0.006, HI = 10.01% (16.66 26.67%, CI = 36.8% (336.06 - 39.74%, BI = 29.97% (73.33 - 103.3%. Health authorities should promote bamboo ovitrap, especially to public as an effort to control Aedes sp.

  13. Presencia de Aedes albopictus en Leticia, Amazonas, Colombia

    Directory of Open Access Journals (Sweden)

    Iván Darío Vélez

    1998-09-01

    Full Text Available La especie Aedes albopictus es un eficiente vector del dengue en Asia y, posiblemente, de otros arbovirus incluyendo la fiebre amarilla y la encefalitis. En la ultima década se ha extendido la distribución de A. albopictus a las Américas. En la ciudad de Tabatinga, Brasil, muy cerceide la frontera con Colombia, se ha informado la presencia de esta especie en 1996 y, posteriormente, en octubre de 1997. En marzo de 1998, en desarrollo de un e&udio sobre enfermedades tropicales realizado por el Programa de Estudio y Cotítrol de Enfermedades Tropicales, PECET, de la Universidad de Antioquia y la secretaría de Salud del Amazonas, se capturaron 8 ejemplares adultos de A. albopictus en la ciudad de Leticia, departamento del Amazonas, siendo éste el primer hallazgo de esta especie en Colombia. La presencia en Colombia de A. albopictus vuelve más complejo el problema del dengue por la mayor adaptación de este vector a climas más fríos, la capacidad de transmisión transovariana, la buena capacidad vectorial para transmitir los cuatro serotipos del virus y la mayor dificultad para su control dado que sus criaderos están en áreas peridomésticas y rurales. La distribución de esta especie tanto en zonas selváticas como rurales y urbanas presenta un peligro potencial de urbanizar la transmisión de la fiebre amarilla. Se presenta el plan de contingencia que se debe implementar por parte de la Secretaría de Salud del Amazonas con miras a evitar la propagación de este vector a otras regiones del país.

  14. The Hill Chart Calculation for Pelton Runner Models using the HydroHillChart - Pelton Module Software

    Directory of Open Access Journals (Sweden)

    Adelina Bostan

    2015-07-01

    Full Text Available The Pelton turbines industrial design is based on the hill chart characteristics obtained by measuring the models. Primary data measurements used to obtain the hill chart can be processed graphically, by hand or by using graphic programs respectively CAD programs; the HydroHillChart - Pelton module software is a specialized tool in achieving the hill chart, using interpolation cubic spline functions. Thereby, based on measurements of several models of Pelton turbines, a computerized library, used to design industrial Pelton turbines can be created. The paper presents the universal characteristics calculated by using the HydroHillChart - Pelton module software for a series of Pelton runners.

  15. Evidence to the Marley Hill Public Inquiry

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, G.K. (Council for the Protection of Rural England (UK). Durham and Teesdale Branch)

    1989-11-01

    George Kenneth Wilson, a retired power station enginer, and holder of such offices as Vice-Chairman of the CPRE Durham and Teesdale Branch, Secretary of the Derwent Valley Protection Society and an officer of the Opencast Mining Intelligence Group, presents reasons for dismissing the appeal for opencast mining at the Marley Hill site in the NE of England saying that the Mineral Planning Guidance Note, MPG3 seemed to be repeating mistakes inherent in the previous 'Plan for coal'. He considers that the application cannot be justified on the grounds of demand or forecast market trends. The type of coal is unsuitable for local power station boilers. The working of the site would destroy a large area of pleasant countryside. The standard of restoration of the 32 sites surrounding Marley Hill is in his opinion very poor.

  16. La Crosse virus infection alters blood feeding behavior in Aedes triseriatus and Aedes albopictus (Diptera: Culicidae).

    Science.gov (United States)

    Jackson, Bryan T; Brewster, Carlyle C; Paulson, Sally L

    2012-11-01

    The effects of La Crosse virus (LACV) infection on blood feeding behavior in Aedes triseriatus (Say) and Aedes albopictus (Skuse) were investigated in the laboratory by measuring the size of the bloodmeal imbibed and the extent of refeeding by virus-infected and uninfected mosquitoes. LACV-infected Ae. triseriatus and Ae. albopictus took significantly less blood compared with uninfected mosquitoes. Twice as many virus-infected Ae. triseriatus mosquitoes refed compared with uninfected individuals (18 vs. 9%; P < 0.05); however, virus infection had no significant effect on the refeeding rate of Ae. albopictus. Reduction in bloodmeal size followed by an increased avidity for refeeding may lead to enhanced horizontal transmission of the LACV by its principal vector, Ae. triseriatus.

  17. Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia

    Czech Academy of Sciences Publication Activity Database

    Bocková, E.; Rudolf, Ivo; Kočišová, A.; Betášová, Lenka; Venclíková, Kristýna; Mendel, Jan; Hubálek, Zdeněk

    2013-01-01

    Roč. 112, č. 10 (2013), s. 3465-3470 ISSN 0932-0113 Institutional support: RVO:68081766 Keywords : Dirofilaria * mosquitoes * Aedes vexans Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.327, year: 2013

  18. Zika virus: Endemic and epidemic ranges of Aedes mosquito transmission

    Directory of Open Access Journals (Sweden)

    David F. Attaway

    2017-01-01

    Full Text Available Summary: As evidence linking Zika virus with serious health complications strengthens, public health officials and clinicians worldwide need to know which locations are likely to be at risk for autochthonous Zika infections. We created risk maps for epidemic and endemic Aedes-borne Zika virus infections globally using a predictive analysis method that draws on temperature, precipitation, elevation, land cover, and population density variables to identify locations suitable for mosquito activity seasonally or year-round. Aedes mosquitoes capable of transmitting Zika and other viruses are likely to live year-round across many tropical areas in the Americas, Africa, and Asia. Our map provides an enhanced global projection of where vector control initiatives may be most valuable for reducing the risk of Zika virus and other Aedes-borne infections. Keywords: Geographic information systems, Geographic information science, Risk mapping, Zika, Aedes modeling

  19. Susceptibility of Aedes aegypti pupae to neem seed kernal extracts ...

    African Journals Online (AJOL)

    Azadirachta indica) seed kernel extracts (NSKE) on Aedes aegypti. The neem seed kernel powder was sequentially extracted with hexane, benzene, ethyl acetate, acetone, DMSO, 2-propanol, ethanol, methanol and dstiledwater.

  20. Characterising the spatial dynamics of sympatric Aedes aegypti and Aedes albopictus populations in the Philippines

    Directory of Open Access Journals (Sweden)

    Jennifer Duncombe

    2013-11-01

    Full Text Available Entomological surveillance and control are essential to the management of dengue fever (DF. Hence, understanding the spatial and temporal patterns of DF vectors, Aedes (Stegomyia aegypti (L. and Ae. (Stegomyia albopictus (Skuse, is paramount. In the Philippines, resources are limited and entomological surveillance and control are generally commenced during epidemics, when transmission is difficult to control. Recent improvements in spatial epidemiological tools and methods offer opportunities to explore more efficient DF surveillance and control solutions: however, there are few examples in the literature from resource-poor settings. The objectives of this study were to: (i explore spatial patterns of Aedes populations and (ii predict areas of high and low vector density to inform DF control in San Jose village, Muntinlupa city, Philippines. Fortnightly, adult female Aedes mosquitoes were collected from 50 double-sticky ovitraps (SOs located in San Jose village for the period June-November 2011. Spatial clustering analysis was performed to identify high and low density clusters of Ae. aegypti and Ae. albopictus mosquitoes. Spatial autocorrelation was assessed by examination of semivariograms, and ordinary kriging was undertaken to create a smoothed surface of predicted vector density in the study area. Our results show that both Ae. aegypti and Ae. albopictus were present in San Jose village during the study period. However, one Aedes species was dominant in a given geographic area at a time, suggesting differing habitat preferences and interspecies competition between vectors. Density maps provide information to direct entomological control activities and advocate the development of geographically enhanced surveillance and control systems to improve DF management in the Philippines.

  1. Aedes Mosquitoes and Aedes-Borne Arboviruses in Africa: Current and Future Threats

    Science.gov (United States)

    Weetman, David; Shearer, Freya M.; Coulibaly, Mamadou

    2018-01-01

    The Zika crisis drew attention to the long-overlooked problem of arboviruses transmitted by Aedes mosquitoes in Africa. Yellow fever, dengue, chikungunya and Zika are poorly controlled in Africa and often go unrecognized. However, to combat these diseases, both in Africa and worldwide, it is crucial that this situation changes. Here, we review available data on the distribution of each disease in Africa, their Aedes vectors, transmission potential, and challenges and opportunities for Aedes control. Data on disease and vector ranges are sparse, and consequently maps of risk are uncertain. Issues such as genetic and ecological diversity, and opportunities for integration with malaria control, are primarily African; others such as ever-increasing urbanization, insecticide resistance and lack of evidence for most control-interventions reflect problems throughout the tropics. We identify key knowledge gaps and future research areas, and in particular, highlight the need to improve knowledge of the distributions of disease and major vectors, insecticide resistance, and to develop specific plans and capacity for arboviral disease surveillance, prevention and outbreak responses. PMID:29382107

  2. Survey on aedes mosquito density and pattern distribution of aedes aegypti and aedes albopictus in high and low incidence districts in north sumatera province

    Science.gov (United States)

    Siregar, Fazidah A.; Makmur, Tri

    2018-03-01

    Transmission and control of dengue hemorrhagic fever are related to its vectors. This study investigated vector density and distribution patterns of Aedes aegypty and Aedes albopictus in Medan and Langkat as high and low incidence district, respectively. An entomological survey was carried out in 304 households both in Medan and Langkat. The results showed that adult Ae. aegypti were predominantly in Medan, while adult Ae. albopictus was only in Langkat. Larvae indices (HI, CI, BI) for Aedes in Medan ( 35,13 and 43) were higher than langkat ( 22,8 and 30). Adult indices (AHI, AD, RR) for Ae. aegypti in Medan and for Ae. albopictus in Langkat were 20,38,24 and 3,5, and 5, respectively. Pattern distribution of Aedes larvae and adult mosquitoes in both district had similar pattern. Aedes larval indices and adult indices both in HIDs and LIDs were above the critical level, indicating potential high risk for DHF transmission. By multiple regression analysis, HI is predictor for DHF transmission in North Sumatera. Thus, in designing an effective control measures for dengue hemorrhagic fever, monitoring distribution and vector density is crucial.

  3. Confidence Hills Mineralogy and Chemin Results from Base of Mt. Sharp, Pahrump Hills, Gale Crater, Mars

    Science.gov (United States)

    Cavanagh, P. D.; Bish, D. L.; Blake, D. F.; Vaniman, D. T.; Morris, R. V.; Ming, D. W.; Rampe, E. B.; Achilles, C. N.; Chipera, S. J.; Treiman, A. H.; hide

    2015-01-01

    The Mars Science Laboratory (MSL) rover Curiosity recently completed its fourth drill sampling of sediments on Mars. The Confidence Hills (CH) sample was drilled from a rock located in the Pahrump Hills region at the base of Mt. Sharp in Gale Crater. The CheMin X-ray diffractometer completed five nights of analysis on the sample, more than previously executed for a drill sample, and the data have been analyzed using Rietveld refinement and full-pattern fitting to determine quantitative mineralogy. Confidence Hills mineralogy has several important characteristics: 1) abundant hematite and lesser magnetite; 2) a 10 angstrom phyllosilicate; 3) multiple feldspars including plagioclase and alkali feldspar; 4) mafic silicates including forsterite, orthopyroxene, and two types of clinopyroxene (Ca-rich and Ca-poor), consistent with a basaltic source; and 5) minor contributions from sulfur-bearing species including jarosite.

  4. Development of Dirofilaria immitis and Dirofilaria repens in Aedes japonicus and Aedes geniculatus

    Directory of Open Access Journals (Sweden)

    Cornelia Silaghi

    2017-02-01

    Full Text Available Abstract Background The mosquito-borne filarial nematodes Dirofilaria immitis and Dirofilaria repens primarily affect dogs but also cats, causing heartworm disease or subcutaneous dirofilariosis, respectively, and both may also cause zoonotic diseases in humans. Several mosquito species have been reported as competent vectors for these nematodes, but no data are available for the invasive mosquito species Aedes japonicus (Theobald, 1901. The objective of this study was to describe the development of both D. immitis and D. repens under standardised experimental laboratory conditions in mosquitoes. Methods For this purpose, both a laboratory strain and field-collected individuals of the invasive mosquito species Ae. japonicus and, for comparative purposes, a laboratory strain of Aedes geniculatus, a rare indigenous species sharing habitats with Ae. japonicus, and of the tropical species Aedes aegypti were used. Anticoagulated microfilariaemic blood was fed at a density of 3000 mf/ml to mosquitoes with a hemotek system. Blood-fed mosquitoes were incubated at 27 °C and 85% relative humidity, and specimens were dissected under the microscope at pre-set time points to observe developmental stages of both Dirofilaria species. Additionally, real-time PCRs were carried out in some microscopically negative samples to determine the infection rates. Results In field-collected Ae. japonicus infectious L3 larvae of both D. immitis and D. repens developed, rendering this mosquito species an efficient vector for both filarial species. Additionally, Ae. geniculatus was shown to be an equally efficient vector for both filarial species. Aedes japonicus mosquitoes from a laboratory colony were refractory to D. immitis but susceptible to D. repens, whereas Ae. aegypti was refractory to both filarial species. Conclusions To our knowledge, Aedes japonicus was for the first time shown to be an efficient vector for both D. immitis and D. repens, indicating that this

  5. Role of Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) in local dengue epidemics in Taiwan.

    Science.gov (United States)

    Tsai, Pui-Jen; Teng, Hwa-Jen

    2016-11-09

    Aedes mosquitoes in Taiwan mainly comprise Aedes albopictus and Ae. aegypti. However, the species contributing to autochthonous dengue spread and the extent at which it occurs remain unclear. Thus, in this study, we spatially analyzed real data to determine spatial features related to local dengue incidence and mosquito density, particularly that of Ae. albopictus and Ae. aegypti. We used bivariate Moran's I statistic and geographically weighted regression (GWR) spatial methods to analyze the globally spatial dependence and locally regressed relationship between (1) imported dengue incidences and Breteau indices (BIs) of Ae. albopictus, (2) imported dengue incidences and BI of Ae. aegypti, (3) autochthonous dengue incidences and BI of Ae. albopictus, (4) autochthonous dengue incidences and BI of Ae. aegypti, (5) all dengue incidences and BI of Ae. albopictus, (6) all dengue incidences and BI of Ae. aegypti, (7) BI of Ae. albopictus and human population density, and (8) BI of Ae. aegypti and human population density in 348 townships in Taiwan. In the GWR models, regression coefficients of spatially regressed relationships between the incidence of autochthonous dengue and vector density of Ae. aegypti were significant and positive in most townships in Taiwan. However, Ae. albopictus had significant but negative regression coefficients in clusters of dengue epidemics. In the global bivariate Moran's index, spatial dependence between the incidence of autochthonous dengue and vector density of Ae. aegypti was significant and exhibited positive correlation in Taiwan (bivariate Moran's index = 0.51). However, Ae. albopictus exhibited positively significant but low correlation (bivariate Moran's index = 0.06). Similar results were observed in the two spatial methods between all dengue incidences and Aedes mosquitoes (Ae. aegypti and Ae. albopictus). The regression coefficients of spatially regressed relationships between imported dengue cases and Aedes mosquitoes

  6. Viruses vector control proposal: genus Aedes emphasis

    Directory of Open Access Journals (Sweden)

    Nelson Nogueira Reis

    2017-07-01

    Full Text Available The dengue fever is a major public health problem in the world. In Brazil, in 2015, there were 1,534,932 cases, being 20,320 cases of severe form, and 811 deaths related to this disease. The distribution of Aedes aegypti, the vector, is extensive. Recently, Zika and Chikungunya viruses had arisen, sharing the same vector as dengue and became a huge public health issue. Without specific treatment, it is urgently required as an effective vector control. This article is focused on reviewing vector control strategies, their effectiveness, viability and economical impact. Among all, the Sterile Insect Technique is highlighted as the best option to be adopted in Brazil, once it is largely effectively used in the USA and Mexico for plagues related to agribusiness.

  7. [Aedes aegypti control strategies: a review].

    Science.gov (United States)

    Zara, Ana Laura de Sene Amâncio; Santos, Sandra Maria Dos; Fernandes-Oliveira, Ellen Synthia; Carvalho, Roberta Gomes; Coelho, Giovanini Evelim

    2016-01-01

    to describe the main strategies to control Aedes aegypti, with emphasis on promising technological innovations for use in Brazil. this study is a non-systematic review of the literature. several technologies have been developed as alternatives in the control of Ae. aegypti, using different mechanisms of action, such as selective monitoring of the infestation, social interventions, dispersing insecticides, new biological control agents and molecular techniques for population control of mosquitoes, also considering the combination between them. Evolving technologies require evaluation of the effectiveness, feasibility and costs of implementation strategies as complementary to the actions already recommended by the National Program for Dengue Control. the integration of different compatible and effective vector control strategies, considering the available technologies and regional characteristics, appears to be a viable method to try to reduce the infestation of mosquitoes and the incidence of arbovirus transmitted by them.

  8. Global genetic diversity of Aedes aegypti.

    Science.gov (United States)

    Gloria-Soria, Andrea; Ayala, Diego; Bheecarry, Ambicadutt; Calderon-Arguedas, Olger; Chadee, Dave D; Chiappero, Marina; Coetzee, Maureen; Elahee, Khouaildi Bin; Fernandez-Salas, Ildefonso; Kamal, Hany A; Kamgang, Basile; Khater, Emad I M; Kramer, Laura D; Kramer, Vicki; Lopez-Solis, Alma; Lutomiah, Joel; Martins, Ademir; Micieli, Maria Victoria; Paupy, Christophe; Ponlawat, Alongkot; Rahola, Nil; Rasheed, Syed Basit; Richardson, Joshua B; Saleh, Amag A; Sanchez-Casas, Rosa Maria; Seixas, Gonçalo; Sousa, Carla A; Tabachnick, Walter J; Troyo, Adriana; Powell, Jeffrey R

    2016-11-01

    Mosquitoes, especially Aedes aegypti, are becoming important models for studying invasion biology. We characterized genetic variation at 12 microsatellite loci in 79 populations of Ae. aegypti from 30 countries in six continents, and used them to infer historical and modern patterns of invasion. Our results support the two subspecies Ae. aegypti formosus and Ae. aegypti aegypti as genetically distinct units. Ae. aegypti aegypti populations outside Africa are derived from ancestral African populations and are monophyletic. The two subspecies co-occur in both East Africa (Kenya) and West Africa (Senegal). In rural/forest settings (Rabai District of Kenya), the two subspecies remain genetically distinct, whereas in urban settings, they introgress freely. Populations outside Africa are highly genetically structured likely due to a combination of recent founder effects, discrete discontinuous habitats and low migration rates. Ancestral populations in sub-Saharan Africa are less genetically structured, as are the populations in Asia. Introduction of Ae. aegypti to the New World coinciding with trans-Atlantic shipping in the 16th to 18th centuries was followed by its introduction to Asia in the late 19th century from the New World or from now extinct populations in the Mediterranean Basin. Aedes mascarensis is a genetically distinct sister species to Ae. aegypti s.l. This study provides a reference database of genetic diversity that can be used to determine the likely origin of new introductions that occur regularly for this invasive species. The genetic uniqueness of many populations and regions has important implications for attempts to control Ae. aegypti, especially for the methods using genetic modification of populations. © 2016 John Wiley & Sons Ltd.

  9. Global Genetic Diversity of Aedes aegypti

    Science.gov (United States)

    Gloria-Soria, Andrea; Ayala, Diego; Bheecarry, Ambicadutt; Calderon-Arguedas, Olger; Chadee, Dave D.; Chiappero, Marina; Coetzee, Maureen; Elahee, Khouaildi bin; Fernandez-Salas, Ildefonso; Kamal, Hany A.; Kamgang, Basile; Khater, Emad I. M.; Kramer, Laura D.; Kramer, Vicki; Lopez-Solis, Alma; Lutomiah, Joel; Martins, Ademir; Micieli, Maria Victoria; Paupy, Christophe; Ponlawat, Alongkot; Rahola, Nil; Rasheed, Syed Basit; Richardson, Joshua B.; Saleh, Amag A.; Sanchez-Casas, Rosa Maria; Seixas, Gonçalo; Sousa, Carla A.; Tabachnick, Walter J.; Troyo, Adriana; Powell, Jeffrey R.

    2016-01-01

    Mosquitoes, especially Aedes aegypti, are becoming important models for studying invasion biology. We characterized genetic variation at 12 microsatellite loci in 79 populations of Ae. aegypti, from 30 countries in six continents and used them to infer historical and modern patterns of invasion. Our results support the two subspecies Ae. aegypti formosus and Ae. aegypti aegypti as genetically distinct units. Ae. aegypti aegypti populations outside Africa are derived from ancestral African populations and are monophyletic. The two subspecies co-occur in both East Africa (Kenya) and West Africa (Senegal). In rural/forest settings (Rabai District of Kenya) the two subspecies remain genetically distinct whereas in urban settings they introgress freely. Populations outside Africa are highly genetically structured likely due to a combination of recent founder effects, discrete discontinuous habitats, and low migration rates. Ancestral populations in sub-Saharan Africa are less genetically structured, as are the populations in Asia. Introduction of Ae. aegypti to the New World coinciding with trans-Atlantic shipping in the 16th to 18th Centuries was followed by its introduction to Asia in the late 19th Century from the New World or from now extinct populations in the Mediterranean Basin. Aedes mascarensis is a genetically distinct sister species to Ae. aegypti s.l.. This study provides a reference database of genetic diversity that can be used to determine the likely origin of new introductions that occur regularly for this invasive species. The genetic uniqueness of many populations and regions has important implications for attempts to control Ae. aegypti, especially for methods using genetic modification of populations. PMID:27671732

  10. Aedes albopictus and the reemergence of Dengue

    Directory of Open Access Journals (Sweden)

    Rezza Giovanni

    2012-01-01

    Full Text Available Abstract Dengue is a vector-borne disease that is estimated to affect millions of individuals each year in tropical and subtropical areas, and it is reemerging in areas that have been disease-free for relatively long periods of time. In this issue of the journal, Peng et al. report on a Dengue outbreak in a city in southern China that had been disease-free for more than two decades. The infection, which was due to serotype 1, was introduced by a traveler from South-east Asia and transmitted by Aedes albopictus, the Asian tiger mosquito. Compared to Aedes aegypti, which is the most important vector of Dengue, Ae albopictus is a less competent vector of arboviruses, and the epidemics it causes are milder. However, Ae albopictus is becoming an increasingly important vector because of its rapidly changing global distribution. In particular, the worldwide trade in second hand tires, which often contain water and are an ideal place for eggs and larvae, has been a key factor in the large-scale conquest of Ae albopictus, which easily adapts to new environments, even in a temperate climate. This expansion is creating new opportunities for viruses to circulate in new areas, becoming a common cause of epidemics in Ae aegypti-free countries, from Hawaii to Mauritius. The outbreak in China, like similar events, was mild and short-lived. Because epidemics due to Ae albopictus are milder, the replacement of Ae aegypti with the tiger mosquito could even result in public-health benefits. However, there is no solid evidence of this, and the milder course of the outbreak could be in part explained by the relatively short duration of the hot season in some affected areas. Since it is almost impossible to prevent Ae albopictus from being introduced in a country, mosquito-control measures at local level remain the most effective means of controlling arbovirus outbreaks.

  11. HydroHillChart – Francis module. Software used to Calculate the Hill Chart of the Francis Hydraulic Turbines

    Directory of Open Access Journals (Sweden)

    Dorian Nedelcu

    2015-07-01

    Full Text Available The paper presents the Hydro Hill Chart - Francis module application, used to calculate the hill chart of the Pelton, Francis and Kaplan hydraulic turbine models, by processing the data measured on the stand. After describing the interface and menu, the input data is graphically presented and the universal characteristic for measuring scenarios ao=const. and n11=const is calculated. Finally, the two calculated hill charts are compared through a graphical superimposition of the isolines.

  12. The Goodwin model: behind the Hill function.

    Directory of Open Access Journals (Sweden)

    Didier Gonze

    Full Text Available The Goodwin model is a 3-variable model demonstrating the emergence of oscillations in a delayed negative feedback-based system at the molecular level. This prototypical model and its variants have been commonly used to model circadian and other genetic oscillators in biology. The only source of non-linearity in this model is a Hill function, characterizing the repression process. It was mathematically shown that to obtain limit-cycle oscillations, the Hill coefficient must be larger than 8, a value often considered unrealistic. It is indeed difficult to explain such a high coefficient with simple cooperative dynamics. We present here molecular models of the standard Goodwin model, based on single or multisite phosphorylation/dephosphorylation processes of a transcription factor, which have been previously shown to generate switch-like responses. We show that when the phosphorylation/dephosphorylation processes are fast enough, the limit-cycle obtained with a multisite phosphorylation-based mechanism is in very good quantitative agreement with the oscillations observed in the Goodwin model. Conditions in which the detailed mechanism is well approximated by the Goodwin model are given. A variant of the Goodwin model which displays sharp thresholds and relaxation oscillations is also explained by a double phosphorylation/dephosphorylation-based mechanism through a bistable behavior. These results not only provide rational support for the Goodwin model but also highlight the crucial role of the speed of post-translational processes, whose response curve are usually established at a steady state, in biochemical oscillators.

  13. New type of hill-top inflation

    Energy Technology Data Exchange (ETDEWEB)

    Barvinsky, A.O.; Nesterov, D.V. [Theory Department, Lebedev Physics Institute, Leninsky Prospect 53, Moscow 119991 (Russian Federation); Kamenshchik, A.Yu., E-mail: barvin@td.lpi.ru, E-mail: Alexander.Kamenshchik@bo.infn.it, E-mail: nesterov@td.lpi.ru [Dipartimento di Fisica and INFN, via Irnerio 46, 40126 Bologna (Italy)

    2016-01-01

    We suggest a new type of hill-top inflation originating from the initial conditions in the form of the microcanonical density matrix for the cosmological model with a large number of quantum fields conformally coupled to gravity. Initial conditions for inflation are set up by cosmological instantons describing underbarrier oscillations in the vicinity of the inflaton potential maximum. These periodic oscillations of the inflaton field and cosmological scale factor are obtained within the approximation of two coupled oscillators subject to the slow roll regime in the Euclidean time. This regime is characterized by rapid oscillations of the scale factor on the background of a slowly varying inflaton, which guarantees smallness of slow roll parameters ε and η of the following inflation stage. A hill-like shape of the inflaton potential is shown to be generated by logarithmic loop corrections to the tree-level asymptotically shift-invariant potential in the non-minimal Higgs inflation model and R{sup 2}-gravity. The solution to the problem of hierarchy between the Planckian scale and the inflation scale is discussed within the concept of conformal higher spin fields, which also suggests the mechanism bringing the model below the gravitational cutoff and, thus, protecting it from large graviton loop corrections.

  14. New type of hill-top inflation

    Energy Technology Data Exchange (ETDEWEB)

    Barvinsky, A.O. [Theory Department, Lebedev Physics Institute,Leninsky Prospect 53, Moscow 119991 (Russian Federation); Department of Physics, Tomsk State University,Lenin Ave. 36, Tomsk 634050 (Russian Federation); Department of Physics and Astronomy, Pacific Institue for Theoretical Physics,University of British Columbia, 6224 Agricultural Road, Vancouver, BC V6T 1Z1 (Canada); Kamenshchik, A.Yu. [Dipartimento di Fisica and INFN,via Irnerio 46, 40126 Bologna (Italy); L.D. Landau Institute for Theoretical Physcis,Kosygin str. 2, 119334 Moscow (Russian Federation); Nesterov, D.V. [Theory Department, Lebedev Physics Institute,Leninsky Prospect 53, Moscow 119991 (Russian Federation)

    2016-01-20

    We suggest a new type of hill-top inflation originating from the initial conditions in the form of the microcanonical density matrix for the cosmological model with a large number of quantum fields conformally coupled to gravity. Initial conditions for inflation are set up by cosmological instantons describing underbarrier oscillations in the vicinity of the inflaton potential maximum. These periodic oscillations of the inflaton field and cosmological scale factor are obtained within the approximation of two coupled oscillators subject to the slow roll regime in the Euclidean time. This regime is characterized by rapid oscillations of the scale factor on the background of a slowly varying inflaton, which guarantees smallness of slow roll parameters ϵ and η of the following inflation stage. A hill-like shape of the inflaton potential is shown to be generated by logarithmic loop corrections to the tree-level asymptotically shift-invariant potential in the non-minimal Higgs inflation model and R{sup 2}-gravity. The solution to the problem of hierarchy between the Planckian scale and the inflation scale is discussed within the concept of conformal higher spin fields, which also suggests the mechanism bringing the model below the gravitational cutoff and, thus, protecting it from large graviton loop corrections.

  15. Effects of tire leachate on the invasive mosquito Aedes albopictus and the native congener Aedes triseriatus

    Directory of Open Access Journals (Sweden)

    Oswaldo C. Villena

    2017-09-01

    Full Text Available Discarded vehicle tire casings are an important artificial habitat for the developmental stages of numerous vector mosquitoes. Discarded vehicle tires degrade under ultraviolet light and leach numerous soluble metals (e.g., barium, cadmium, zinc and organic substances (e.g., benzothiazole and its derivatives [BZTs], polyaromatic hydrocarbons [PAHs] that could affect mosquito larvae that inhabit the tire casing. This study examined the relationship between soluble zinc, a common marker of tire leachate, on mosquito densities in tire habitats in the field, and tested the effects of tire leachate on the survival and development of newly hatched Aedes albopictus and Aedes triseriatus larvae in a controlled laboratory dose-response experiment. In the field, zinc concentrations were as high as 7.26 mg/L in a single tire and averaged as high as 2.39 (SE ± 1.17 mg/L among tires at a single site. Aedes albopictus (37/42 tires, 81.1% and A. triseriatus (23/42, 54.8% were the most widespread mosquito species, co-occurred in over half (22/42, 52.4% of all tires, and A. triseriatus was only collected without A. albopictus in one tire. Aedes triseriatus was more strongly negatively associated with zinc concentration than A. albopictus, and another common mosquito, C. pipiens, which was found in 17 tires. In the laboratory experiment, A. albopictus per capita rate of population change (λ′ was over 1.0, indicating positive population growth, from 0–8.9 mg/L zinc concentration (0–10,000 mg/L tire leachate, but steeply declined to zero from 44.50–89.00 mg/L zinc (50,000–100,000 mg/L tire leachate. In contrast, A. triseriatus λ′ declined at the lower concentration of 0.05 mg/L zinc (100 mg/L tire leachate, and was zero at 0.45, 8.90, 44.50, and 89.00 mg/L zinc (500, 10,000, 50,000 and 100,000 mg/L tire leachate. These results indicate that tire leachate can have severe negative effects on populations of container-utilizing mosquitoes at

  16. Aedes aegypti: modelo experimental de atividade biológica de fitoprodutos

    Directory of Open Access Journals (Sweden)

    Michele Teixeira Serdeiro

    2017-06-01

    Full Text Available Aedes aegypti (Linnaeus, 1762 é reconhecido como transmissor de várias arboviroses de importância na saúde pública, como a dengue, zika, chikungunya e febre amarela urbana. O principal método de prevenir a transmissão desses vírus ainda é o controle do mosquito vetor. Produtos naturais de origem vegetal vêm sendo investigados, como mais uma ferramenta no controle de vetores, e compostos menos impactantes ao meio ambiente e a saúde humana. Devido à importância deste culicídeo, buscou-se extrato e frações de C. catharinensis com atividade larvicida sobre Ae. aegypti. O extrato bruto metanólico (EBM e sua fração (EBM 1 obtidos da embaúba foram aplicados no meio de criação das larvas (L3 nas concentrações de 10, 30 e 50 μg/mL. O tratamento com C. cahtarinensis resultou na alteração do período de desenvolvimento larval, pupal e de L3-adulto do mosquito. A mortalidade pupal (25% foi obtida pela fração EBM1. Este estudo demonstrou a eficácia de C. catharinensis sobre o período de desenvolvimento de Ae. aegypti.

  17. Weak Properties and Robustness of t-Hill Estimators

    Czech Academy of Sciences Publication Activity Database

    Jordanova, P.; Fabián, Zdeněk; Hermann, P.; Střelec, L.; Rivera, A.; Girard, S.; Torres, S.; Stehlík, M.

    2016-01-01

    Roč. 19, č. 4 (2016), s. 591-626 ISSN 1386-1999 Institutional support: RVO:67985807 Keywords : asymptotic properties of estimators * point estimation * t-Hill estimator * t-lgHill estimator Subject RIV: BB - Applied Statistics, Operational Research Impact factor: 1.679, year: 2016

  18. 78 FR 73187 - Black Hills National Forest Advisory Board

    Science.gov (United States)

    2013-12-05

    ... DEPARTMENT OF AGRICULTURE Forest Service Black Hills National Forest Advisory Board AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Black Hills National Forest Advisory Board (Board... all members of the Advisory Board; (2) provide orientation to Board Members on Basic Laws governing...

  19. Plants profile of Malakand Pass Hills, District Malakand, Pakistan

    African Journals Online (AJOL)

    use

    2011-11-21

    Nov 21, 2011 ... famous Malakand fort guards the road on both sides of the pass. Malakand is a ... Underneath Malakand Hills, the waters of the River Swat ... southern sloping faces of the hills. They speak ...... Pinus roxburghii Sarg. Antidote ...

  20. 27 CFR 9.162 - Sta. Rita Hills.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Sta. Rita Hills. 9.162.... Rita Hills. (a) Name. The name of the viticultural area described in this section is “Sta. Rita Hills”. For purposes of part 4 of this chapter, “Sta. Rita Hills” is a term of viticultural significance. (b...

  1. Spectral parameter power series representation for Hill's discriminant

    International Nuclear Information System (INIS)

    Khmelnytskaya, K.V.; Rosu, H.C.

    2010-01-01

    We establish a series representation of the Hill discriminant based on the spectral parameter power series (SPPS) recently introduced by Kravchenko. We also show the invariance of the Hill discriminant under a Darboux transformation and employing the Mathieu case the feasibility of this type of series for numerical calculations of the eigenspectrum.

  2. Microhabitats of Merriam's turkeys in the Black Hills, South Dakota

    Science.gov (United States)

    Mark A. Rumble; Stanley H. Anderson

    1996-01-01

    Merriam’s Turkeys (Meleagris gallopavo merriami) are associated with ponderosa pine (Pinus ponderosa) forests in the western United States, but are not native to the ponderosa pine forest of the Black Hills, South Dakota. The Black Hills population was established by transplanting birds from New Mexico and Colorado between 1948 and...

  3. The montane forest associated amphibian species of the Taita Hills ...

    African Journals Online (AJOL)

    The montane forest associated amphibian species of the Taita Hills, Kenya. ... They are surrounded by the dry Tsavo plains. ... The biodiversity importance of the Taita Hills lies with the number of endemics per unit of area of remaining forest, ...

  4. Influence of larval and pupal products on the oviposition behavior of Aedes Fluviatilis (Lutz (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Rotraut A. G. B. Consoli

    1988-06-01

    Full Text Available Several larval and pupal products of Aedes fluviatilis (Lutz were tested for their influence on the oviposition behaviour of females of the same species. Significant (alfa = 0,05 atractiveness was shown by: larval water, previously containing 5 to 15 larvae/1,5 ml; larval water, preserved up to 38 days; evaporate and reconstructed larval water extracts up to 2 years after production and water filtered through fresh or dried ground larvae. hexanic larval water extracts and water filtered through fresh or dired ground pupae did not influence oviposition.Estudou-se a influência sobre o comportamento de oviposição das fêmeas de Aedes fluviatilis (Lutz de produtos derivados das formas imaturas da mesma espécie. As fêmeas foram atraídas significativamente (x=0,05 por ocasião da ovoposição por: água destilada que contivera 5 ou 15 larvas/1,5 ml, a mesma água (5 larvas/1,5 mlapós sua preservação por 38 dias; extratos evaporados e reconstituídos de água que conteve larvas, por até dois anos a sua produção, e filtrados de macerados frescos e secos de larvas. Extratos hexânicos de água que conteve larvas e filtrados de macerados descos e secos de pupas não atraíram a ovoposição das fêmeas.

  5. Aedes (Stegomyia) aegypti and Aedes (Howardina) cozumelensis in Yucatán State, México, with a summary of published collection records for Ae. cozumelensis

    OpenAIRE

    García-Rejón, Julián E.; López-Uribe, Mildred P.; Loroño-Pino, María Alba; Arana-Guardia, Roger; Puc-Tinal, Maria; López-Uribe, Genny M.; Coba-Tún, Carlos; Baak-Baak, Carlos M.; Machain-Williams, Carlos; Reyes-Solis, Guadalupe C.; Lozano-Fuentes, Saul; Saavedra-Rodriguez, Karla; Black IV, William C.; Beaty, Barry J.; Eisen, Lars

    2012-01-01

    We collected mosquito immatures from artificial containers during 2010–2011 from 26 communities, ranging in size from small rural communities to large urban centers, located in different parts of Yucatán State in southeastern México. The arbovirus vector Aedes (Stegomyia) aegypti was collected from all 26 examined communities, and nine of the communities also yielded another container-inhabiting Aedes mosquito: Aedes (Howardina) cozumelensis. The communities from which Ae. cozumelensis were c...

  6. Mathematical model of temephos resistance in Aedes aegypti mosquito population

    Science.gov (United States)

    Aldila, D.; Nuraini, N.; Soewono, E.; Supriatna, A. K.

    2014-03-01

    Aedes aegypti is the main vector of dengue disease in many tropical and sub-tropical countries. Dengue became major public concern in these countries due to the unavailability of vaccine or drugs for dengue disease in the market. Hence, the only way to control the spread of DF and DHF is by controlling the vectors carrying the disease, for instance with fumigation, temephos or genetic manipulation. Many previous studies conclude that Aedes aegypti may develop resistance to many kind of insecticide, including temephos. Mathematical model for transmission of temephos resistance in Aedes aegypti population is discussed in this paper. Nontrivial equilibrium point of the system and the corresponding existence are shown analytically. The model analysis have shown epidemiological trends condition that permits the coexistence of nontrivial equilibrium is given analytically. Numerical results are given to show parameter sensitivity and some cases of worsening effect values for illustrating possible conditions in the field.

  7. Effect of temperature on the population dynamics of Aedes aegypti

    Science.gov (United States)

    Yusoff, Nuraini; Tokachil, Mohd Najir

    2015-10-01

    Aedes aegypti is one of the main vectors in the transmission of dengue fever. Its abundance may cause the spread of the disease to be more intense. In the study of its biological life cycle, temperature was found to increase the development rate of each stage of this species and thus, accelerate the process of the development from egg to adult. In this paper, a Lefkovitch matrix model will be used to study the stage-structured population dynamics of Aedes aegypti. In constructing the transition matrix, temperature will be taken into account. As a case study, temperature recorded at the Subang Meteorological Station for year 2006 until 2010 will be used. Population dynamics of Aedes aegypti at maximum, average and minimum temperature for each year will be simulated and compared. It is expected that the higher the temperature, the faster the mosquito will breed. The result will be compared to the number of dengue fever incidences to see their relationship.

  8. Stage-Structured Population Dynamics of AEDES AEGYPTI

    Science.gov (United States)

    Yusoff, Nuraini; Budin, Harun; Ismail, Salemah

    Aedes aegypti is the main vector in the transmission of dengue fever, a vector-borne disease affecting world population living in tropical and sub-tropical countries. Better understanding of the dynamics of its population growth will help in the efforts of controlling the spread of this disease. In looking at the population dynamics of Aedes aegypti, this paper explored the stage-structured modeling of the population growth of the mosquito using the matrix population model. The life cycle of the mosquito was divided into five stages: eggs, larvae, pupae, adult1 and adult2. Developmental rates were obtained for the average Malaysian temperature and these were used in constructing the transition matrix for the matrix model. The model, which was based only on temperature, projected that the population of Aedes aegypti will blow up with time, which is not realistic. For further work, other factors need to be taken into account to obtain a more realistic result.

  9. Zika virus: Endemic and epidemic ranges of Aedes mosquito transmission.

    Science.gov (United States)

    Attaway, David F; Waters, Nigel M; Geraghty, Estella M; Jacobsen, Kathryn H

    As evidence linking Zika virus with serious health complications strengthens, public health officials and clinicians worldwide need to know which locations are likely to be at risk for autochthonous Zika infections. We created risk maps for epidemic and endemic Aedes-borne Zika virus infections globally using a predictive analysis method that draws on temperature, precipitation, elevation, land cover, and population density variables to identify locations suitable for mosquito activity seasonally or year-round. Aedes mosquitoes capable of transmitting Zika and other viruses are likely to live year-round across many tropical areas in the Americas, Africa, and Asia. Our map provides an enhanced global projection of where vector control initiatives may be most valuable for reducing the risk of Zika virus and other Aedes-borne infections. Copyright © 2016 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Ltd. All rights reserved.

  10. Multiple blood feeding and host-seeking behavior in Aedes aegypti and Aedes albopictus (Diptera: Culicidae).

    Science.gov (United States)

    Farjana, Thahsin; Tuno, Nobuko

    2013-07-01

    The body size of mosquitoes can influence a number of bionomic factors, such as their blood-feeding ability, host attack rate, and fecundity. All of these traits are important determinants of their potential to transmit diseases. Among abiotic and biotic factors, high temperature and low nutrition in the developing stages of mosquitoes generally result in small adults. We studied the relationship between body size and multiple feeding in a gonotrophic cycle and some fecundity attributes by using three strains of two competent vector species, Aedes aegypti (L.) and Aedes albopictus (Skuse). We raised small and large mosquitoes under low and high food conditions in the laboratory to measure parameters of fecundity and blood-feeding behavior. Fecundity was positively correlated with body size in both species, whereas the number of bloodmeals, the frequency of host-seeking behavior, and egg retention were negatively correlated with body size in the Ae. albopictus Nagasaki strain. We found that multiple feeding and host-seeking behavior were negatively correlated with body size, i.e., small mosquitoes tended to have more contact with hosts. We found that two mechanisms that inhibit engorged mosquitoes from seeking out hosts, distension-induced and oocyte-induced inhibition, were not strong enough to limit host-seeking behavior, and multiple feeding increased fecundity. Size-dependent multiple feeding and host-seeking behavior affect contact frequency with hosts and should be considered when predicting how changes in mosquito body size affect disease transmission.

  11. Invasiveness of Aedes aegypti and Aedes albopictus and Vectorial Capacity for Chikungunya Virus.

    Science.gov (United States)

    Lounibos, Leon Philip; Kramer, Laura D

    2016-12-15

    In this review, we highlight biological characteristics of Aedes aegypti and Aedes albopictus, 2 invasive mosquito species and primary vectors of chikungunya virus (CHIKV), that set the tone of these species' invasiveness, vector competence, and vectorial capacity (VC). The invasiveness of both species, as well as their public health threats as vectors, is enhanced by preference for human blood. Vector competence, characterized by the efficiency of an ingested arbovirus to replicate and become infectious in the mosquito, depends largely on vector and virus genetics, and most A. aegypti and A. albopictus populations thus far tested confer vector competence for CHIKV. VC, an entomological analog of the pathogen's basic reproductive rate (R 0 ), is epidemiologically more important than vector competence but less frequently measured, owing to challenges in obtaining valid estimates of parameters such as vector survivorship and host feeding rates. Understanding the complexities of these factors will be pivotal in curbing CHIKV transmission. © The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

  12. Differentiation of Aedes aegypti and Aedes notoscriptus (Diptera: Culicidae) eggs using scanning electron microscopy.

    Science.gov (United States)

    Faull, Katherine J; Williams, Craig R

    2016-05-01

    Aedes notoscriptus and Aedes aegypti are both peri-domestic, invasive container-breeding mosquitoes. While the two potential arboviral vectors are bionomically similar, their sympatric distribution in Australia is limited. In this study, analyses of Ae. aegypti and Ae. notoscriptus eggs were enabled using scanning electron microscopy. Significant variations in egg length to width ratio and outer chorionic cell field morphology between Ae. aegypti and Ae. notoscriptus enabled distinction of the two species. Intraspecific variations in cell field morphology also enabled differentiation of the separate populations of both species, highlighting regional and global variation. Our study provides a comprehensive comparative analysis of inter- and intraspecific egg morphological and morphometric variation between two invasive container-breeding mosquitoes. The results indicate a high degree of intraspecific variation in Ae. notoscriptus egg morphology when compared to the eggs of Ae. aegypti. Comparative morphological analyses of Ae. aegypti and Ae. notoscriptus egg attributes using SEM allows differentiation of the species and may be helpful in understanding egg biology in relation to biotope of origin. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Widespread evidence for interspecific mating between Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in nature.

    Science.gov (United States)

    Bargielowski, I E; Lounibos, L P; Shin, D; Smartt, C T; Carrasquilla, M C; Henry, A; Navarro, J C; Paupy, C; Dennett, J A

    2015-12-01

    Aedes aegypti and Aedes albopictus, two important vectors of the dengue and chikungunya viruses to humans, often come in contact in their invasive ranges. In these circumstances, a number of factors are thought to influence their population dynamics, including resource competition among the larval stages, prevailing environmental conditions and reproductive interference in the form of satyrization. As the distribution and abundance of Ae. aegypti and Ae. albopictus have profound epidemiological implications, understanding the competitive interactions that influence these patterns in nature is important. While evidence for resource competition and environmental factors had been gathered from the field, the evidence for reproductive interference, though strongly inferred through laboratory trials, remained sparse (one small-scale field trial). In this paper we demonstrate that low rates (1.12-3.73%) of interspecific mating occur in nature among populations of these species that have co-existed sympatrically from 3 to 150yrs. Finally this report contributes a new species-specific primer set for identifying the paternity of sperm extracted from field collected specimens. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. A new tent trap for monitoring the daily activity of Aedes aegypti and Aedes albopictus.

    Science.gov (United States)

    Casas Martínez, Mauricio; Orozco Bonilla, Arnoldo; Muñoz Reyes, Miguel; Ulloa García, Armando; Bond, J Guillermo; Valle Mora, Javier; Weber, Manuel; Rojas, Julio C

    2013-12-01

    In this study, we designed a new tent trap; the BioDiVector (BDV) tent trap, consisting of two rectangular tents that use human bait without endangering the technical personnel. The daily activity pattern of Aedes aegypti and Aedes albopictus in intra, peri, and extradomiciliary sites was studied in an endemic area of dengue in southern Mexico by using the BDV tent trap. Totals of 3,128 individuals of Ae. aegypti and 833 Ae. albopictus were captured. More Ae. aegypti males than females were caught, while the opposite was true with Ae. albopictus. The activity of both mosquito species was affected by the interaction between the collection site and time of day. In general, more individuals of both mosquito species were captured at the extradomicillary sites than at the peri and intradomicillary sites. Mosquitoes showed two peaks of activity, one in the morning and the other in the afternoon, but in general this only occurred at the extradomicillary sites, whereas no peak of activity was observed at the intra and peridomicillary sites. Overall, Ae. aegypti had a higher indirect biting rate than Ae. albopictus. Finally, due to its efficiency, simplicity, and low cost, we suggest the use of this innovative tool for entomological surveillance, bionomics and vector incrimination studies in geographical areas where dengue and other arboviruses are present. © 2013 The Society for Vector Ecology.

  15. EKSISTENSI DAN SEBARAN NYAMUK AEDES AEGYPTI DAN AEDES ALBOPICTUS DI KAMPUS UNIVERSITAS HASANUDDIN MAKASSAR

    Directory of Open Access Journals (Sweden)

    Yosefina Dota T

    2015-03-01

    Full Text Available Penelitian yang bertujuan untuk mengetahui eksistensi dan sebaran nyamuk Aedes aegypti dan Aedes albopictus telah dilakukan di Kampus Universitas Hasanuddin, Kec. Tamalanrea, Makassar. Penelitian bersifat eksploratif dengan melakukan sampling terhadap lima lokasi yaitu : a Fak. Peternakan (Utara, b Fak. Hukum (Timur, c Pusat Kegiatan Penelitian/PKP (Selatan, d Workshop/Dekat Pondokan mahasiswa (Barat dan e Fak. MIPA (Tengah. Sampling nyamuk menggunakan metode ovitrap (menggunakan attraktan Eluisine Indica L. dan survei terhadap berbagai tempat penampungan air. Sampel telur dan larva nyamuk yang diperoleh disimpan dalam microtube berisi alkohol 70% kemudian diidentifikasi berdasarkan Rueda (2004. Hasil penelitian menunjukkan bahwa Nyamuk Ae. aegypti dan Ae. albopictus ditemukan hidup dan berkembang biak di kampus Universitas Hasanuddin, Makassar. Eksistensi dan sebaran kedua jenis nyamuk tersebut dipengaruhi oleh faktor adanya manusia/masyarakat kampus yang beraktivitas baik di dalam ruangan (indoor maupun di luar ruangan (outdoor, adanya berbagai tempat penampungan air baik buatan (bak mandi, ember maupun barang bekas (botol/kaleng bekas, tempurung kelapa, vegetasi/tanaman dan berbagai macam hewan yang berada di sekitaran kampus. Hasil penelitian dalam ruangan (indoor menunjukkan bahwa nyamuk Ae. aegypti lebih banyak ditemukan hidup di dalam ruangan gedung PKP sedangkan Ae.albopictus lebih banyak di Fak. Hukum. Hasil penelitian di luar ruangan (outdoor menunjukkan bahwa nyamuk Ae. aegypti lebih banyak ditemukan hidup di area Workshop sedangkan Ae.albopictus lebih banyak di area PKP.

  16. Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands

    Directory of Open Access Journals (Sweden)

    Cao-Lormeau Van-Mai

    2009-03-01

    Full Text Available Abstract Dengue virus (DENV, the etiological agent of dengue fever, is transmitted to the human host during blood uptake by an infective mosquito. Infection of vector salivary glands and further injection of infectious saliva into the human host are key events of the DENV transmission cycle. However, the molecular mechanisms of DENV entry into the mosquito salivary glands have not been clearly identified. Otherwise, although it was demonstrated for other vector-transmitted pathogens that insect salivary components may interact with host immune agents and impact the establishment of infection, the role of mosquito saliva on DENV infection in human has been only poorly documented. To identify salivary gland molecules which might interact with DENV at these key steps of transmission cycle, we investigated the presence of proteins able to bind DENV in salivary gland extracts (SGE from two mosquito species. Using virus overlay protein binding assay, we detected several proteins able to bind DENV in SGE from Aedes aegypti (L. and Aedes polynesiensis (Marks. The present findings pave the way for the identification of proteins mediating DENV attachment or entry into mosquito salivary glands, and of saliva-secreted proteins those might be bound to the virus at the earliest step of human infection. The present findings might contribute to the identification of new targets for anti-dengue strategies.

  17. Motivations of female Black Hills deer hunters

    Science.gov (United States)

    Gigliotti, Larry M.; Covelli Metcalf, Elizabeth

    2016-01-01

    State fish and wildlife agencies are particularly interested in attracting female participation because of the potential to offset declining participation in hunting. Understanding female hunters’ motivations will be critical for designing effective recruitment and retention programs for women hunters. Although female participation in hunting is increasing, males still outnumber females by about tenfold. Gender differences in deer hunters were explored by comparing ratings of eight motivations (social, nature, excitement, meat, challenge, trophy, extra hunting opportunity, and solitude). Hunter types were defined by hunters’ selection of the most important motivation for why they like Black Hills deer hunting. Overall, females and males were relatively similar in their ratings of the eight motivations, and we found 85% gender similarity in the selection of the most important motivation. Women were slightly more motivated by the food aspect of the hunt while men placed slightly more value on the hunt as a sporting activity.

  18. Oviposition ecology and species composition of Aedes spp. and Aedes aegypti dynamics in variously urbanized settings in arbovirus foci in southeastern Côte d'Ivoire.

    Science.gov (United States)

    Zahouli, Julien B Z; Utzinger, Jürg; Adja, Maurice A; Müller, Pie; Malone, David; Tano, Yao; Koudou, Benjamin G

    2016-09-29

    Aedes mosquito-transmitted outbreaks of dengue and yellow fever have been reported from rural and urban parts of Côte d'Ivoire. The present study aimed at assessing Aedes spp. oviposition ecology in variously urbanized settings within arbovirus foci in southeastern Côte d'Ivoire. Aedes spp. eggs were sampled using a standard ovitrap method from January 2013 to April 2014 in different ecosystems of rural, suburban and urban areas. Emerged larvae were reared until the adult stage for species identification. Aedes spp. oviposition ecology significantly varied from rural-to-urban areas and according to the ecozones and the seasons. Species richness of Aedes spp. gradually decreased from rural (eight species) to suburban (three species) and urban (one species) areas. Conversely, emerged adult Aedes spp. mean numbers were higher in the urban (1.97 Aedes/ovitrap/week), followed by the suburban (1.44 Aedes/ovitrap/week) and rural (0.89 Aedes/ovitrap/week) areas. Aedes aegypti was the only species in the urban setting (100 %), and was also the predominant species in suburban (85.5 %) and rural (63.3 %) areas. The highest Ae. aegypti mean number was observed in the urban (1.97 Ae. aegypti/ovitrap/week), followed by the suburban (1.20 Ae. aegypti/ovitrap/week) and rural (0.57 Ae. aegypti/ovitrap/week) areas. Aedes africanus (9.4 %), Ae. dendrophilus (8.0 %), Ae. metallicus (1.3 %) in the rural, and Ae. vittatus (6.5 %) and Ae. metallicus (1.2 %) in the suburban areas each represented more than 1 % of the total Aedes fauna. In all areas, Aedes species richness and abundance were higher in the peridomestic zones and during the rainy season, with stronger variations in species richness in the rural and in abundance in the urban areas. Besides, the highest Culex quinquefasciatus abundance was found in the urban areas, while Eretmapodites chrysogaster was restricted to the rural areas. Urbanization correlates with a substantially higher abundance in Aedes mosquitoes and

  19. HydroHillChart – Pelton module. Software used to Calculate the Hill Chart of the Pelton Hydraulic Turbines

    OpenAIRE

    Dorian Nedelcu; Adelina Bostan; Florin Peris-Bendu

    2015-01-01

    The paper presents the HydroHillChart - Pelton module application, used to calculate the hill chart of the Pelton hydraulic turbine models, by processing the data measured on the stand. In addition, the tools offered by the application such as: interface, menu, input data, numerical and graphical results, etc. are described.

  20. HydroHillChart – Pelton module. Software used to Calculate the Hill Chart of the Pelton Hydraulic Turbines

    Directory of Open Access Journals (Sweden)

    Dorian Nedelcu

    2015-07-01

    Full Text Available The paper presents the HydroHillChart - Pelton module application, used to calculate the hill chart of the Pelton hydraulic turbine models, by processing the data measured on the stand. In addition, the tools offered by the application such as: interface, menu, input data, numerical and graphical results, etc. are described.

  1. Ash and Steam, Soufriere Hills Volcano, Monserrat

    Science.gov (United States)

    2002-01-01

    International Space Station crew members are regularly alerted to dynamic events on the Earth's surface. On request from scientists on the ground, the ISS crew observed and recorded activity from the summit of Soufriere Hills on March 20, 2002. These two images provide a context view of the island (bottom) and a detailed view of the summit plume (top). When the images were taken, the eastern side of the summit region experienced continued lava growth, and reports posted on the Smithsonian Institution's Weekly Volcanic Activity Report indicate that 'large (50-70 m high), fast-growing, spines developed on the dome's summit. These spines periodically collapsed, producing pyroclastic flows down the volcano's east flank that sometimes reached the Tar River fan. Small ash clouds produced from these events reached roughly 1 km above the volcano and drifted westward over Plymouth and Richmond Hill. Ash predominately fell into the sea. Sulfur dioxide emission rates remained high. Theodolite measurements of the dome taken on March 20 yielded a dome height of 1,039 m.' Other photographs by astronauts of Montserrat have been posted on the Earth Observatory: digital photograph number ISS002-E-9309, taken on July 9, 2001; and a recolored and reprojected version of the same image. Digital photograph numbers ISS004-E-8972 and 8973 were taken 20 March, 2002 from Space Station Alpha and were provided by the Earth Sciences and Image Analysis Laboratory at Johnson Space Center. Additional images taken by astronauts and cosmonauts can be viewed at the NASA-JSC Gateway to Astronaut Photography of Earth.

  2. Flotation process control optimisation at Prominent Hill

    International Nuclear Information System (INIS)

    Lombardi, Josephine; Muhamad, Nur; Weidenbach, M.

    2012-01-01

    OZ Minerals' Prominent Hill copper- gold concentrator is located 130 km south east of the town of Coober Pedy in the Gawler Craton of South Australia. The concentrator was built in 2008 and commenced commercial production in early 2009. The Prominent Hill concentrator is comprised of a conventional grinding and flotation processing plant with a 9.6 Mtpa ore throughput capacity. The flotation circuit includes six rougher cells, an IseMill for regrinding the rougher concentrate and a Jameson cell heading up the three stage conventional cell cleaner circuit. In total there are four level controllers in the rougher train and ten level controllers in the cleaning circuit for 18 cells. Generic proportional — integral and derivative (PID) control used on the level controllers alone propagated any disturbances downstream in the circuit that were generated from the grinding circuit, hoppers, between cells and interconnected banks of cells, having a negative impact on plant performance. To better control such disturbances, FloatStar level stabiliser was selected for installation on the flotation circuit to account for the interaction between the cells. Multivariable control was also installed on the five concentrate hoppers to maintain consistent feed to the cells and to the IsaMill. An additional area identified for optimisation in the flotation circuit was the mass pull rate from the rougher cells. FloatStar flow optimiser was selected to be installed subsequent to the FloatStar level stabiliser. This allowed for a unified, consistent and optimal approach to running the rougher circuit. This paper describes the improvement in the stabilisation of the circuit achieved by the FloatStar level stabiliser by using the interaction matrix between cell level controllers and the results and benefits of implementing the FloatStar flow optimiser on the rougher train.

  3. First record of Aedes koreicus (Diptera, Culicidae) in Slovenia.

    Science.gov (United States)

    Kalan, Katja; Šušnjar, Jana; Ivović, Vladimir; Buzan, Elena

    2017-08-01

    The first record of Aedes koreicus was made in the village of Lovrenc na Dravskem Polju, north-eastern part of the country. The discovery of Ae. koreicus in various continental European countries motivated us to revise samples of the collected Aedes japonicus japonicus. We found Ae. koreicus in samples from 2013, where the larvae were misidentified as Ae. j. japonicus. The species was identified morphologically and molecularly. The first discovery of Ae. koreicus advocates an urgent need for a nationwide mosquito surveillance programme.

  4. Aedes nigrinus (Eckstein, 1918 (Diptera, Culicidae, a new country record for England, contrasted with Aedes sticticus (Meigen, 1838

    Directory of Open Access Journals (Sweden)

    Ralph E. Harbach

    2017-04-01

    Full Text Available We report the discovery of Aedes nigrinus (Eckstein, 1918 in the New Forest of southern England, bringing to 36 the number of mosquito species recorded in Britain. Because it seems that this species has been misidentified previously in Britain as the morphologically similar Aedes sticticus (Meigen, 1838, the two species are contrasted and distinguished based on distinctive differences exhibited in the adult and larval stages. The pupa of Ae. nigrinus is unknown, but the pupa of Ae. sticticus is distinguished from the pupae of other species of Aedes by modification of the most recent key to British mosquitoes. The history of the mosquito fauna recorded in the UK is summarized and bionomical information is provided for the two species.

  5. Susceptibilidade de Aedes aegypti aos inseticidas temephos e cipermetrina, Brasil Susceptibility of Aedes aegypti to temephos and cypermethrin insecticides, Brazil

    Directory of Open Access Journals (Sweden)

    Jonny E Duque Luna

    2004-12-01

    Full Text Available Realizaram-se bioensaios para detectar a susceptibilidade de Aedes aegypti aos inseticidas químicos, temefós e cipermetrina. Os resultados mostraram que esta espécie é suscetível a temefós e apresenta resistência a cipermetrinae.Bioassays were performed in order to detect the susceptibility of Aedes aegypti to the chemical insecticides temephos and cypermethrin. The results showed that this species is susceptible to temephos and presents resistance to cypermethrin.

  6. Insecticide susceptibility of Aedes aegypti and Aedes albopictus in Central Africa

    Directory of Open Access Journals (Sweden)

    Nwane Philippe

    2011-05-01

    Full Text Available Abstract Background Aedes aegypti (Linnaeus, 1762 and Aedes albopictus (Skuse, 1894 are the main vectors of dengue (DENV and chikungunya (CHIKV viruses worldwide. As there is still no vaccine or specific treatment for DENV and CHIKV, vector control remains the cornerstone of prevention and outbreak control. Unfortunately, vector control programs are facing operational challenges with mosquitoes becoming resistant to commonly used insecticides in several areas through the world. Throughout Central Africa no recent data are available susceptible/resistant status of either vector species since the introduction/arrival of Ae. albopictus in this area. We therefore studied the level of resistance of these two major vectors to insecticides commonly used in Africa for mosquito control. Results Aedes aegypti and Ae. albopictus were sampled in six urban localities of Cameroon (Garoua, Bertoua, Yaoundé, Bafia, Buea and Gabon (Libreville. Larval bioassays, carried out to determine the lethal concentrations (LC50 and LC95 and resistance ratios (RR50 and RR95 suggested that both vector species were susceptible to Bti (Bacillus thuringiensis var israeliensis and temephos. Bioassays were also performed on adults using WHO diagnostic test kits to assess phenotypic resistance to deltamethrin, DDT, fenitrothion and propoxur. These experiments showed that one population of Ae. aegypti (Libreville and two populations of Ae. albopictus (Buea and Yaoundé were resistant to DDT (mortality 36% to 71%. Resistance to deltamethrin was also suspected in Ae. albopictus from Yaoundé (83% mortality. All other field mosquito populations were susceptible to deltamethrin, DDT, fenitrothion and propoxur. No increase in the knockdown times (Kdt50 and Kdt95 was noted in the Yaoundé resistant population compared to other Ae. albopictus populations, suggesting the possible involvement of metabolic resistance to deltamethrin and DDT. Conclusion In view of the recent increase in

  7. Funny hills in pion spectra from heavy-ion collisions

    International Nuclear Information System (INIS)

    Rasmussen, J.O.

    1982-03-01

    A discussion of some of the systematic features of the pion spectra in heavy-ions reactions is given. A discussion of the hills and valleys in heavy ion pion spectra that show up at the lower pion energies is given. The following topics are discussed: (1) three kinds of funny hills; (2) π - / + ratios near center of mass; (3) new Monte Carlo studies of charged pion spectra; and (4) pion orbiting about fireballs and Bose-Einstein behavior as explanation for the mid-rapidity P/sub perpendicular to/ approx. = 0.4 to 0.5 m/sub π/c hill

  8. TNO en Aedes over voordelen regisserend opdrachtgeverschap bij planmatig onderhoud

    NARCIS (Netherlands)

    Arts, S.

    2016-01-01

    Regisserend opdrachtgeverschap is voor corporaties een goede manier om met planmatig onderhoud om te gaan. Dat stellen Stefan Arts van TNO en Maarten Georgius van Aedes, die onderzoek deden naar dat nieuwe opdrachtgeverschap. Hoe het moet, staat helder op papier. Maar de praktijk is weerbarstig, zo

  9. A review on symmetries for certain Aedes aegypti models

    Science.gov (United States)

    Freire, Igor Leite; Torrisi, Mariano

    2015-04-01

    We summarize our results related with mathematical modeling of Aedes aegypti and its Lie symmetries. Moreover, some explicit, group-invariant solutions are also shown. Weak equivalence transformations of more general reaction diffusion systems are also considered. New classes of solutions are obtained.

  10. Breeding of Aedes albopictus Skuse (Diptera: Culicidae) in water ...

    African Journals Online (AJOL)

    of adult mosquitoes, with humans acting as baits, was employed for the study. Environmental inspection and collection of larvae in the water-holding containers around the buildings were used to study the breeding sites of the mosquitoes. A total of 73 adult mosquitoes, made up of Aedes albopictus (76.71%) and A. aegypti ...

  11. The larvicidal activities of three Jatropha species against Aedes ...

    African Journals Online (AJOL)

    ... reports the larvicidal activities of the petroleum ether, ethylacetate and methanol extracts of the leaves and stem barks of the three plants and the column chromatographic fractions of the petroleum ether extract of J. gossipifolia stem bark against the larvae of Aedes aegypti mosquito, the vector of dengue and yellow fevers ...

  12. Invasion of cemeteries in Florida by Aedes albopictus.

    Science.gov (United States)

    O'Meara, G F; Gettman, A D; Evans, L F; Scheel, F D

    1992-03-01

    Aedes albopictus has been found in 53 of the 67 Florida counties. The initial discoveries in 11 of these counties were made in cemeteries. At several locations, Ae. albopictus became well-established in cemeteries before appearing in nearby accumulations of waste tires. The recycling of plastic floral baskets may be aiding the spread of Ae. albopictus. Mosquitoes were commonly found in all types of flower-holding containers in cemeteries, except bronze vases. In the laboratory, most Aedes aegypti eggs laid in bronze vases hatched, but larvae subsequently died. The spread of Ae. albopictus in cemeteries seems to occur at the expense of Ae. aegypti populations. At one cemetery immature Ae. albopictus and Ae. aegypti were found in about 70% of the Aedes-positive containers at the start of a monitoring program. In subsequent collections from this site, Ae. albopictus was found in nearly all Aedes-positive containers, whereas there was a progressive decrease in containers with Ae. aegypti. This trend did not appear to be the result of any seasonal pattern because in a nearby cemetery where Ae. albopictus was absent, Ae. aegypti did not show a similar decline. Limiting flower-holding containers to those with drain holes or to bronze vases would greatly limit mosquito production.

  13. Evolution of the Puente Hills Thrust Fault

    Science.gov (United States)

    Bergen, K. J.; Shaw, J. H.; Dolan, J. F.

    2013-12-01

    This study aims to assess the evolution of the blind Puente Hills thrust fault system (PHT) by determining its age of initiation, lateral propagation history, and changes in slip rate over time. The PHT presents one of the largest seismic hazards in the United States, given its location beneath downtown Los Angeles. The PHT is comprised of three fault segments: the Los Angeles (LA), Santa Fe Springs (SFS), and Coyote Hills (CH). The LA and SFS segments are characterized by growth stratigraphy where folds formed by uplift on the fault segments have been continually buried by sediment from the Los Angeles and San Gabriel rivers. The CH segment has developed topography and is characterized by onlapping growth stratigraphy. This depositional setting gives us the unique opportunity to measure uplift on the LA and SFS fault segments, and minimum uplift on the CH fault segment, as the difference in sediment thicknesses across the buried folds. We utilize depth converted oil industry seismic reflection data to image the fold geometries. Identifying time-correlative stratigraphic markers for slip rate determination in the basin has been a problem for researchers in the past, however, as the faunal assemblages observed in wells are time-transgressive by nature. To overcome this, we utilize the sequence stratigraphic model and well picks of Ponti et al. (2007) as a basis for mapping time-correlative sequence boundaries throughout our industry seismic reflection data from the present to the Pleistocene. From the Pleistocene to Miocene we identify additional sequence boundaries in our seismic reflection data from imaged sequence geometries and by correlating industry well formation tops. The sequence and formation top picks are then used to build 3-dimensional surfaces in the modeling program Gocad. From these surfaces we measure the change in thicknesses across the folds to obtain uplift rates between each sequence boundary. Our results show three distinct phases of

  14. Presence and Potential Distribution of Aedes albopictus and Aedes japonicus japonicus (Diptera: Culicidae) in Slovenia.

    Science.gov (United States)

    Kalan, Katja; Ivovic, Vladimir; Glasnovic, Peter; Buzan, Elena

    2017-11-07

    In Slovenia, two invasive mosquito species are present, Aedes albopictus (Skuse, 1895) (Diptera: Culicidae) and Aedes japonicus (Theobald, 1901) (Diptera: Culicidae). In this study, we examined their actual distribution and suitable habitats for new colonizations. Data from survey of species presence in 2013 and 2015, bioclimatic variables and altitude were used for the construction of predictive maps. We produced various models in Maxent software and tested two bioclimatic variable sets, WorldClim and CHELSA. For the variable selection of A. albopictus modeling we used statistical and expert knowledge-based approach, whereas for A. j. japonicus we used only a statistically based approach. The best performing models for both species were chosen according to AIC score-based evaluation. In 2 yr of sampling, A. albopictus was largely confined to the western half of Slovenia, whereas A. j. japonicus spread significantly and can be considered as an established species in a large part of the country. Comparison of models with WorldClim and CHELSA variables for both species showed models with CHELSA variables as a better tool for prediction. Finally, we validated the models performance in predicting distribution of species according to collected field data. Our study confirms that both species are co-occurring and are sympatric in a large part of the country area. The tested models could be used for future prevention of invasive mosquitoes spreading in other countries with similar bioclimatic conditions. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  15. Weak Larval Competition Between Two Invasive Mosquitoes Aedes koreicus and Aedes albopictus (Diptera: Culicidae).

    Science.gov (United States)

    Baldacchino, Frédéric; Arnoldi, Daniele; Lapère, Charlotte; Rosà, Roberto; Montarsi, Fabrizio; Capelli, Gioia; Rizzoli, Annapaola

    2017-09-01

    Aedes (Hulecoeteomyia) koreicus (Edwards) and Aedes (Stegomyia) albopictus (Skuse) are two invasive mosquito species well established in northeastern Italy, and these two species may co-occur in artificial larval habitats such as tires, buckets, drums, and catch basins. Because Ae. albopictus has been shown experimentally to be a superior competitor to several mosquito species, we investigated larval competition between Ae. koreicus and Ae. albopictus using two diet levels (low level and high level) and 10 Ae. albopictus: Ae. koreicus density combination levels (30:0, 60:0, 15:15, 30:30, 10:20, 20:10, 20:40, 40:20, 0:60, and 0:30). A multivariate analysis (MANOVA) demonstrated a significant effect of the density combination on Ae. koreicus survivorship, female development time, and female wing length considered simultaneously in low-level diet and high-level diet treatments. Pairwise comparisons across low-level diet treatments showed a significant reduction of Ae. koreicus survivorship in 20:10 combination treatments (i.e. 20 Ae. albopictus and 10 Ae. koreicus larvae) compared to 10:20, 20:40, and 30:30 combination treatments, while no difference was detected for Ae. albopictus between density combination treatments. Furthermore, Ae. albopictus developed faster than Ae. koreicus regardless of diet and density combination treatments. Our results show weak larval competition between Ae. koreicus and Ae. albopictus with a slight advantage of the latter species. On the other hand, the presence of Ae. albopictus seems to favor the emergence of larger Ae. koreicus females. We suggest that factors such as habitats preferences or seasonal distributions may be determinant for the invasion success of Ae. koreicus. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  16. Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus.

    Directory of Open Access Journals (Sweden)

    Thais Chouin-Carneiro

    2016-03-01

    Full Text Available Since the major outbreak in 2007 in the Yap Island, Zika virus (ZIKV causing dengue-like syndromes has affected multiple islands of the South Pacific region. In May 2015, the virus was detected in Brazil and then spread through South and Central America. In December 2015, ZIKV was detected in French Guiana and Martinique. The aim of the study was to evaluate the vector competence of the mosquito spp. Aedes aegypti and Aedes albopictus from the Caribbean (Martinique, Guadeloupe, North America (southern United States, South America (Brazil, French Guiana for the currently circulating Asian genotype of ZIKV isolated from a patient in April 2014 in New Caledonia.Mosquitoes were orally exposed to an Asian genotype of ZIKV (NC-2014-5132. Upon exposure, engorged mosquitoes were maintained at 28° ± 1 °C, a 16h:8h light:dark cycle and 80% humidity. 25-30 mosquitoes were processed at 4, 7 and 14 days post-infection (dpi. Mosquito bodies (thorax and abdomen, heads and saliva were analyzed to measure infection, dissemination and transmission, respectively. High infection but lower disseminated infection and transmission rates were observed for both Ae. aegypti and Ae. albopictus. Ae. aegypti populations from Guadeloupe and French Guiana exhibited a higher dissemination of ZIKV than the other Ae. aegypti populations examined. Transmission of ZIKV was observed in both mosquito species at 14 dpi but at a low level.This study suggests that although susceptible to infection, Ae. aegypti and Ae. albopictus were unexpectedly low competent vectors for ZIKV. This may suggest that other factors such as the large naïve population for ZIKV and the high densities of human-biting mosquitoes contribute to the rapid spread of ZIKV during the current outbreak.

  17. Evaluation of Alternative Killing Agents for Aedes aegypti (Diptera: Culicidae) in the Gravid Aedes Trap (GAT).

    Science.gov (United States)

    Heringer, Laila; Johnson, Brian J; Fikrig, Kara; Oliveira, Bruna A; Silva, Richard D; Townsend, Michael; Barrera, Roberto; Eiras, Álvaro E; Ritchie, Scott A

    2016-07-01

    The Gravid Aedes Trap (GAT) uses visual and olfactory cues to attract gravid Aedes aegypti (L.) that are then captured when knocked down by a residual pyrethroid surface spray. However, the use of surface sprays can be compromised by poor availability of the spray and pesticide resistance in the target mosquito. We investigated several "alternative" insecticide and insecticide-free killing agents for use in the GAT. This included long-lasting insecticide-impregnated nets (LLINs), vapor-active synthetic pyrethroids (metofluthrin), canola oil, and two types of dry adhesive sticky card. During bench top assays LLINs, metofluthrin, and dry sticky cards had 24-h knockdown (KD) percentages >80% (91.2 ± 7.2%, 84.2 ± 6.8%, and 83.4 ± 6.1%, respectively), whereas the 24-h KD for canola oil was 70 ± 7.7%, which improved to 90.0 ± 3.7% over 48 h. Importantly, there were no significant differences in the number of Ae. aegypti collected per week or the number of traps positive for Ae. aegypti between the sticky card and canola oil treatments compared with the surface spray and LLIN treatments in semifield and field trials. These results demonstrate that the use of inexpensive and widely available insecticide-free agents such as those described in this study are effective alternatives to pyrethroids in regions with insecticide-resistant populations. The use of such environmentally friendly insecticide-free alternatives will also be attractive in areas where there is substantial resistance to insecticide use due to environmental and public health concerns. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. Public Computer Usage in Chapel Hill Public Library

    Data.gov (United States)

    Town of Chapel Hill, North Carolina — Data collected November 2014 - May 2016. As of June 2016, this data is no longer collected on a continual basis.This dataset includes frequency and length of use of...

  19. 78 FR 21098 - Black Hills National Forest Advisory Board

    Science.gov (United States)

    2013-04-09

    ... Hills National Forest Advisory Board (Board) will meet in Rapid City South Dakota. The Board is... staff before the meeting. The agenda will include time for people to make oral statements of three...

  20. USGS Hill Shade Base Map Service from The National Map

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — USGS Hill Shade (or Shaded Relief) is a tile cache base map created from the National Elevation Dataset (NED), a seamless dataset of best available raster elevation...

  1. "Beverly Hills 90210" : kes mida teeb / Tiina Lepiste

    Index Scriptorium Estoniae

    Lepiste, Tiina

    2003-01-01

    Aaron Spellingu produtseeritud ja 2000. aastal lõpetatud menuseriaalis "Beverly Hills 90210" osalenud näitlejate edaspidisest elust seoses plaaniga teha täispikk mängufilm "10 Year High School Reunion"

  2. The effect of photoperiod on life history and blood-feeding activity in Aedes albopictus and Aedes aegypti (Diptera: Culicidae).

    Science.gov (United States)

    Costanzo, K S; Schelble, S; Jerz, K; Keenan, M

    2015-06-01

    Several studies have examined how climatic variables such as temperature and precipitation may affect life history traits in mosquitoes that are important to disease transmission. Despite its importance as a seasonal cue in nature, studies investigating the influence of photoperiod on such traits are relatively few. This study aims to investigate how photoperiod alters life history traits, survival, and blood-feeding activity in Aedes albopictus (Skuse) and Aedes aegypti (Linnaeus). We performed three experiments that tested the effects of day length on female survival, development time, adult size, fecundity, adult life span, and propensity to blood feed in Ae. albopictus and Ae. aegypti. Each experiment had three photoperiod treatments: 1) short-day (10L:14D), 2) control (12L:12D), and 3) long-day (14L:10D). Aedes albopictus adult females were consistently larger in size when reared in short-day conditions. Aedes aegypti adult females from short-day treatments lived longer and were more likely to take a blood meal compared to other treatments. We discuss how species-specific responses may reflect alternative strategies evolved to increase survival during unfavorable conditions. We review the potential impacts of these responses on seasonal transmission patterns, such as potentially increasing vectorial capacity of Ae. aegypti during periods of shorter day lengths. © 2015 The Society for Vector Ecology.

  3. Stormwater Management Plan for the Arden Hills Army Training Site, Arden Hills, Minnesota

    Energy Technology Data Exchange (ETDEWEB)

    Carr, Adrianne E. [Argonne National Lab. (ANL), Argonne, IL (United States); Wuthrich, Kelsey K. [Argonne National Lab. (ANL), Argonne, IL (United States); Ziech, Angela M. [Argonne National Lab. (ANL), Argonne, IL (United States); Bowen, Esther E. [Argonne National Lab. (ANL), Argonne, IL (United States); Quinn, John [Argonne National Lab. (ANL), Argonne, IL (United States)

    2013-03-01

    This stormwater management plan focuses on the cantonment and training areas of the Arden Hills Army Training Site (AHATS). The plan relates the site stormwater to the regulatory framework, and it summarizes best management practices to aide site managers in promoting clean site runoff. It includes documentation for a newly developed, detailed model of stormwater flow retention for the entire AHATS property and adjacent upgradient areas. The model relies on established modeling codes integrated in a U.S. Department of Defense-sponsored software tool, the Watershed Modeling System (WMS), and it can be updated with data on changes in land use or with monitoring data.

  4. After runaway: The trans-Hill stage of planetesimal growth

    International Nuclear Information System (INIS)

    Lithwick, Yoram

    2014-01-01

    When planetesimals begin to grow by coagulation, they first enter an epoch of runaway, during which the biggest bodies grow faster than all the others. The questions of how runaway ends and what comes next have not been answered satisfactorily. We show that runaway is followed by a new stage—the 'trans-Hill stage'—that commences when the bodies that dominate viscous stirring ('big bodies') become trans-Hill, i.e., when their Hill velocity matches the random speed of the small bodies they accrete. Subsequently, the small bodies' random speed grows in lockstep with the big bodies' sizes, such that the system remains in the trans-Hill state. Trans-Hill growth is crucial for determining the efficiency of growing big bodies, as well as their growth timescale and size spectrum. Trans-Hill growth has two sub-stages. In the earlier one, which occurs while the stirring bodies remain sufficiently small, the evolution is collisionless, i.e., collisional cooling among all bodies is irrelevant. The efficiency of forming big bodies in this collisionless sub-stage is very low, ∼10α << 1, where α ∼ 0.005(a/AU) –1 is the ratio between the physical size of a body and its Hill radius. Furthermore, the size spectrum is flat (equal mass per size decade, i.e., q = 4). This collisionless trans-Hill solution explains results from previous coagulation simulations for both the Kuiper Belt and the asteroid belt. The second trans-Hill sub-stage commences once the stirring bodies grow big enough (>α –1 × the size of the accreted small bodies). After that time, collisional cooling among small bodies controls the evolution. The efficiency of forming big bodies rises and the size spectrum becomes more top heavy. Trans-Hill growth can terminate in one of two ways, depending on the sizes of the small bodies. First, mutual accretion of big bodies can become significant and conglomeration proceeds until half of the total mass is converted into big bodies

  5. Biogeography of the Shimba Hills ecosystem herpetofauna in Kenya.

    Science.gov (United States)

    Malonza, Patrick K; Mulwa, David M; Nyamache, Joash O; Jones, Georgina

    2018-03-18

    The Shimba Hills ecosystem along the south coast of Kenya is a key East African biodiversity hotspot. Historically, it is biogeographically assignable to the East African coastal biome. We examined the current Shimba Hills herpetofauna and their zoogeographical affinities to the coastal forests and nearby Eastern Arc Mountains biodiversity hotspots. The key studied sites included the Shimba Hills National Reserve, forest reserves, Kaya forests, and adjacent private land. Data on herpetofaunal richness were obtained from recent field surveys, literature, and specimens held at the National Museums of Kenya, Herpetology Section Collection, Nairobi. The Makadara, Mwele, and Longo-Mwagandi forests within the Shimba Hills National Reserve hosted the highest number of unique and rare species. Generally, the forest reserves and Kaya forests were important refuges for forest-associated species. On private land, Mukurumudzi Dam riparian areas were the best amphibian habitat and were host to three IUCN (Red List) Endangered-EN amphibian species, namely, Boulengerula changamwensis, Hyperolius rubrovermiculatus, and Afrixalus sylvaticus, as well as one snake species Elapsoidea nigra. Using herpetofauna as zoogeographic indicators, the Shimba Hills were determined to be at a crossroads between the coastal forests (13 endemic species) and the Eastern Arc Mountains (seven endemic species). Most of the Eastern Arc Mountains endemic species were from recent records, and thus more are likely to be found in the future. This 'hybrid' species richness pattern is attributable to the hilly topography of the Shimba Hills and their proximity to the Indian Ocean. This has contributed to the Shimba Hills being the richest herpetofauna area in Kenya, with a total of 89 and 36 reptile and amphibian species, respectively. Because of its unique zoogeography, the Shimba Hills ecosystem is undoubtedly a key biodiversity area for conservation investment.

  6. Archeological Investigations at Big Hill Lake, Southeastern Kansas, 1980.

    Science.gov (United States)

    1982-09-01

    settled primarily along the Neosho river and Labette, Big Hill, and Pumpkin creeks. One of the first settlers in Osage township, in which Big Hill...slabs is not known at present. About 10 years later, in 1876, materials were reported- ly collected from an aboriginal site along Pumpkin creek...and length- ening its lifetime of use. As would therefore be expected, cracks are present between each of the paired holes on both of the two restored

  7. The Bradford Hill considerations on causality: a counterfactual perspective

    Directory of Open Access Journals (Sweden)

    Höfler Michael

    2005-11-01

    Full Text Available Abstract Bradford Hill's considerations published in 1965 had an enormous influence on attempts to separate causal from non-causal explanations of observed associations. These considerations were often applied as a checklist of criteria, although they were by no means intended to be used in this way by Hill himself. Hill, however, avoided defining explicitly what he meant by "causal effect". This paper provides a fresh point of view on Hill's considerations from the perspective of counterfactual causality. I argue that counterfactual arguments strongly contribute to the question of when to apply the Hill considerations. Some of the considerations, however, involve many counterfactuals in a broader causal system, and their heuristic value decreases as the complexity of a system increases; the danger of misapplying them can be high. The impacts of these insights for study design and data analysis are discussed. The key analysis tool to assess the applicability of Hill's considerations is multiple bias modelling (Bayesian methods and Monte Carlo sensitivity analysis; these methods should be used much more frequently.

  8. Amostragem por larva-única na vigilância de Aedes aegypti Single-larva sampling for Aedes aegypti surveillance

    Directory of Open Access Journals (Sweden)

    José Eduardo Bracco

    1995-04-01

    Full Text Available Com a finalidade de testar a metodologia de amostragem por larva-única na vigilância entomológica do Aedes aegypti, foram pesquisados domicílios do Município de Araraquara, SP (Brasil. Nos criadouros que continham larvas de Aedes uma delas foi coletada. Como controle, após a coleta da larva-única, todas as larvas foram coletadas para identificação posterior. Esse processo foi repetido no laboratório. Dos 447 domicílios visitados, apenas 12 foram considerados positivos e 20 criadouros foram identificados; destes, 13 continham larvas de Aedes; 5, larvas de Aedes e Culex e 2, larvas de Culex. Os resultados mostram o reconhecimento correto, no campo, de todos os criadouros, evidenciando que o método poderia ser utilizado na vigilância entomológica de municípios sem infestação domiciliar ou infestados apenas com uma única espécie de Aedes.Buildings in Araraquara city, Southeastern Brazil, were searched during a year for the presence of Aedes larvae using single larva sampling in order to check the single-larva methodology. In those breeding places in wich Aedes larvae were found, one of them was collected. As a control, after the single larva had been collected, all the larvae from the breeding place were collected for later identification. This process was repeated in the laboratory. Of the 447 domiciles searched, 12 were considered positive and 20 breeding places were found. Of the breeding places, 13 contained Aedes larvae, 5 both Aedes and Culex larvae and 2 Culex larvae only. The results show that all the breeding places in the field were properly recognited showing the method may be used for Aedes surveillance in cities infested with one species only or without any domiciliary infestation.

  9. The Maxillary Palp of Aedes aegypti, a Model of Multisensory Integration

    Science.gov (United States)

    2014-01-01

    The maxillary palp of Aedes aegypti, a model of multisensory integration Jonathan D. Bohbot, Jackson T. Sparks, Joseph C. Dickens* United States...24 February 2014 Keywords: Aedes aegypti Olfaction Mosquito Maxillary palp Thermosensation Mechanosensation a b s t r a c t Female yellow-fever...mosquitoes, Aedes aegypti, are obligate blood-feeders and vectors of the pathogens that cause dengue fever, yellow fever and Chikungunya. This feeding

  10. Toxicity of Thiophenes from Echinops transiliensis (Asteraceae) against Aedes aegypti (Diptera: Culicidae) Larvae

    Science.gov (United States)

    2014-01-01

    Toxicity of Thiophenes from Echinops transiliensis (Asteraceae) against Aedes aegypti (Diptera: Culicidae) Larvae by Hiroshi Nakano*a)b)c), Abbas...larvicides against Aedes aegypti. Structural differences among compounds 3, 5, and 8 consisted in differing AcO and OH groups attached to C(3’’) and C(4...serious human diseases including malaria, Japanese encephalitis, yellow fever, dengue, and filariasis. The urban-adapted Aedes aegypti mosquito has become

  11. Global risk mapping for major diseases transmitted by Aedes aegypti and Aedes albopictus.

    Science.gov (United States)

    Leta, Samson; Beyene, Tariku Jibat; De Clercq, Eva M; Amenu, Kebede; Kraemer, Moritz U G; Revie, Crawford W

    2018-02-01

    The objective of this study was to map the global risk of the major arboviral diseases transmitted by Aedes aegypti and Aedes albopictus by identifying areas where the diseases are reported, either through active transmission or travel-related outbreaks, as well as areas where the diseases are not currently reported but are nonetheless suitable for the vector. Data relating to five arboviral diseases (Zika, dengue fever, chikungunya, yellow fever, and Rift Valley fever (RVF)) were extracted from some of the largest contemporary databases and paired with data on the known distribution of their vectors, A. aegypti and A. albopictus. The disease occurrence data for the selected diseases were compiled from literature dating as far back as 1952 to as recent as 2017. The resulting datasets were aggregated at the country level, except in the case of the USA, where state-level data were used. Spatial analysis was used to process the data and to develop risk maps. Out of the 250 countries/territories considered, 215 (86%) are potentially suitable for the survival and establishment of A. aegypti and/or A. albopictus. A. albopictus has suitability foci in 197 countries/territories, while there are 188 that are suitable for A. aegypti. There is considerable variation in the suitability range among countries/territories, but many of the tropical regions of the world provide high suitability over extensive areas. Globally, 146 (58.4%) countries/territories reported at least one arboviral disease, while 123 (49.2%) reported more than one of the above diseases. The overall numbers of countries/territories reporting autochthonous vector-borne occurrences of Zika, dengue, chikungunya, yellow fever, and RVF, were 85, 111, 106, 43, and 39, respectively. With 215 countries/territories potentially suitable for the most important arboviral disease vectors and more than half of these reporting cases, arboviral diseases are indeed a global public health threat. The increasing proportion of

  12. Biogeography of the two major arbovirus mosquito vectors, Aedes aegypti and Aedes albopictus (Diptera, Culicidae, in Madagascar

    Directory of Open Access Journals (Sweden)

    Raharimalala Fara

    2012-03-01

    Full Text Available Abstract Background In the past ten years, the Indian Ocean region has been the theatre of severe epidemics of chikungunya and dengue. These outbreaks coincided with a high increase in populations of Aedes albopictus that outcompete its sister taxon Aedes aegypti in most islands sampled. The objective of this work was to update the entomological survey of the two Aedes species in the island of Madagascar which has to face these arboviroses. Methods The sampling of Aedes mosquitoes was conducted during two years, from October 2007 to October 2009, in fifteen localities from eight regions of contrasting climates. Captured adults were identified immediately whereas immature stages were bred until adult stage for determination. Phylogenetic analysis was performed using two mtDNA genes, COI and ND5 and trees were constructed by the maximum likelihood (ML method with the gene time reversible (GTR model. Experimental infections with the chikungunya virus strain 06.21 at a titer of 107.5 pfu/mL were performed to evaluate the vector competence of field-collected mosquitoes. Disseminated infection rates were measured fourteen days after infection by immunofluorescence assay performed on head squashes. Results The species Aedes aegypti was detected in only six sites in native forests and natural reserves. In contrast, the species Aedes albopictus was found in 13 out of the 15 sites sampled. Breeding sites were mostly found in man-made environments such as discarded containers, used tires, abandoned buckets, coconuts, and bamboo cuts. Linear regression models showed that the abundance of Ae. albopictus was significantly influenced by the sampling region (F = 62.00, p -16 and period (F = 36.22, p = 2.548 × 10-13, that are associated with ecological and climate variations. Phylogenetic analysis of the invasive Ae. albopictus distinguished haplotypes from South Asia and South America from those of Madagascar, but the markers used were not discriminant enough

  13. Sodium Channel Mutations and Pyrethroid Resistance in Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Yuzhe Du

    2016-10-01

    Full Text Available Pyrethroid insecticides are widely used to control insect pests and human disease vectors. Voltage-gated sodium channels are the primary targets of pyrethroid insecticides. Mutations in the sodium channel have been shown to be responsible for pyrethroid resistance, known as knockdown resistance (kdr, in various insects including mosquitoes. In Aedes aegypti mosquitoes, the principal urban vectors of dengue, zika, and yellow fever viruses, multiple single nucleotide polymorphisms in the sodium channel gene have been found in pyrethroid-resistant populations and some of them have been functionally confirmed to be responsible for kdr in an in vitro expression system, Xenopus oocytes. This mini-review aims to provide an update on the identification and functional characterization of pyrethroid resistance-associated sodium channel mutations from Aedes aegypti. The collection of kdr mutations not only helped us develop molecular markers for resistance monitoring, but also provided valuable information for computational molecular modeling of pyrethroid receptor sites on the sodium channel.

  14. Identification of germline transcriptional regulatory elements in Aedes aegypti

    Science.gov (United States)

    Akbari, Omar S.; Papathanos, Philippos A.; Sandler, Jeremy E.; Kennedy, Katie; Hay, Bruce A.

    2014-02-01

    The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmitting mosquitoes with individuals refractory to disease transmission, or bringing about population suppression. Several of these systems, including Medea, UDMEL, and site-specific nucleases, which can be used to drive genes into populations or bring about population suppression, utilize transcriptional regulatory elements that drive germline-specific expression. Here we report the identification of multiple regulatory elements able to drive gene expression specifically in the female germline, or in the male and female germline, in the mosquito Aedes aegypti. These elements can also be used as tools with which to probe the roles of specific genes in germline function and in the early embryo, through overexpression or RNA interference.

  15. Factores que modifican los índices larvarios de Aedes aegypti en Colima, México Factors that modify the larval indices of Aedes aegypti in Colima, Mexico

    Directory of Open Access Journals (Sweden)

    Francisco Espinoza Gómez

    2001-07-01

    Full Text Available Objetivos. Con el propósito de explorar las variables que pudieran tener mayor impacto sobre los índices larvarios de Aedes aegypti, principal vector del dengue, se realizó un estudio descriptivo y longitudinal en la ciudad mexicana de Colima, ubicada en la costa central del Pacífico. Métodos. Se inspeccionaron 187 domicilios en los que se determinó el índice de viviendas (IV y el número de contenedores positivos por casa (C+/C durante las temporadas lluviosa y seca. Como variables independientes se analizaron la temperatura ambiental, la temporada, la aplicación de malatión en rociados a volumen ultrabajo (ULV, el índice de calidad de la vivienda (ICV y el grado de conocimientos, actitudes y prácticas (CAP. Resultados. Tanto el análisis de regresión logística multivariada como el de regresión lineal múltiple mostraron una reducción del IV y del C+/C atribuible a un efecto de la temporada seca. Paradójicamente, la temperatura mostró una correlación negativa con los índices larvarios, la cual se hizo más aparente en la temporada seca. El ICV tuvo la mayor asociación con el IV y con el C+/C, independientemente de las demás variables. Las nebulizaciones de malatión mostraron un discreto efecto negativo sobre los índices, mientras que los CAP no mostraron ninguna asociación con ellos. Conclusiones. Se concluye que la temperatura alta puede reducir el número de criaderos durante la temporada seca, que el ICV puede ser un buen estimador de la infestación por Ae. aegypti, que el uso de malatión ULV reduce los criaderos y que el índice de CAP presenta escasa asociación con la presencia de los mismos.Objective. In order to investigate the variables that could have the greatest impact on larval indices of Aedes aegypti, the main vector of dengue, a descriptive, longitudinal study was carried out in the city of Colima, which is located in the Mexican Pacific coastal state of the same name. Methods. A total of 187 dwellings

  16. History of domestication and spread of Aedes aegypti - A Review

    OpenAIRE

    Jeffrey R Powell; Walter J Tabachnick

    2013-01-01

    The adaptation of insect vectors of human diseases to breed in human habitats (domestication) is one of the most important phenomena in medical entomology. Considerable data are available on the vector mosquito Aedes aegypti in this regard and here we integrate the available information including genetics, behaviour, morphology, ecology and biogeography of the mosquito, with human history. We emphasise the tremendous amount of variation possessed by Ae. aegypti for virtually all traits consid...

  17. Larvicidal activity of Cybistax antisyphilitica against Aedes aegypti larvae.

    Science.gov (United States)

    Rodrigues, A M S; de Paula, J E; Roblot, F; Fournet, A; Espíndola, L S

    2005-12-01

    The larvicidal activity against Aedes aegypti larvae of a stem wood hexane extract of Cybistax antisyphilitica was evaluated. Bioassay-guided fractionation of the crude extract, monitored by larvicidal assay, led to the isolation of a natural quinone identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1.4-naphthoquinone (lapachol). This compound was quite potent against A. aegypti larvae (LC50 26.3 microg/ml).

  18. Intraspecific Competition and Population Dynamics of Aedes aegypti

    Science.gov (United States)

    Paixão, C. A.; Charret, I. C.; Lima, R. R.

    2012-04-01

    We report computational simulations for the evolution of the population of the dengue vector, Aedes aegypti mosquitoes. The results suggest that controlling the mosquito population, on the basis of intraspecific competition at the larval stage, can be an efficient mechanism for controlling the spread of the epidemic. The results also show the presence of a kind of genetic evolution in vector population, which results mainly in increasing the average lifespan of individuals in adulthood.

  19. Similarity solutions for systems arising from an Aedes aegypti model

    Science.gov (United States)

    Freire, Igor Leite; Torrisi, Mariano

    2014-04-01

    In a recent paper a new model for the Aedes aegypti mosquito dispersal dynamics was proposed and its Lie point symmetries were investigated. According to the carried group classification, the maximal symmetry Lie algebra of the nonlinear cases is reached whenever the advection term vanishes. In this work we analyze the family of systems obtained when the wind effects on the proposed model are neglected. Wide new classes of solutions to the systems under consideration are obtained.

  20. First record of Aedes (Stegomyia) albopictus in Belgium.

    Science.gov (United States)

    Schaffner, Francis; Van Bortel, Wim; Coosemans, Marc

    2004-06-01

    The 1st record of Aedes albopictus in Belgium was made in a village in Oost-Vlaanderen Province. Two preimaginal stages were collected on October 31, 2000, in the used tire stock of a recycling company that imports tires from the USA and Japan. The species has reproduced on site, and local environmental conditions make its establishment possible. Anopheles plumbeus was a common companion species found in tires in high densities.

  1. Vector Competence of French Polynesian Aedes aegypti and Aedes polynesiensis for Zika Virus.

    Directory of Open Access Journals (Sweden)

    Vaea Richard

    2016-09-01

    Full Text Available In 2013-2014, French Polynesia experienced for the first time a Zika outbreak. Two Aedes mosquitoes may have contributed to Zika virus (ZIKV transmission in French Polynesia: the worldwide distributed Ae. aegypti and the Polynesian islands-endemic Ae. polynesiensis mosquito.To evaluate their vector competence for ZIKV, mosquitoes were infected per os at viral titers of 7 logs tissue culture infectious dose 50%. At several days post-infection (dpi, saliva was collected from each mosquito and inoculated onto C6/36 mosquito cells to check for the presence of ZIKV infectious particles. Legs and body of each mosquito were also collected and submitted separately to RNA extraction and ZIKV RT-PCR. In Ae. aegypti the infection rate was high as early as 6 dpi and the dissemination efficiency get substantial from 9 dpi while the both rates remained quite low in Ae. polynesiensis. The transmission efficiency was poor in Ae. aegypti until 14 dpi and no infectious saliva was found in Ae. polynesiensis at the time points studied.In our experimental conditions, the late ability of the French Polynesian Ae. aegypti to transmit ZIKV added by the poor competence of Ae. polynesiensis for this virus suggest the possible contribution of another vector for the propagation of ZIKV during the outbreak, in particular in remote islands where Ae. polynesiensis is predominating.

  2. Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Muñoz

    2013-01-01

    Full Text Available The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV through receptors of midgut epithelial cells. The envelope protein (E of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized to bind cell receptors. In order to identify potential receptors, proteins from mosquito midgut tissue and C6/36 cells were purified by affinity using columns with the recombinant E protein domain III (rE-DIII or DENV particles bound covalently to Sepharose 4B to compare and evaluate their performance to bind proteins including putative receptors from female mosquitoes of Ae. aegypti. To determine their identity mass spectrometric analysis of purified proteins separated by polyacrylamide gel electrophoresis was performed. Our results indicate that both viral particles and rE-DIII bound proteins with the same apparent molecular weights of 57 and 67 kDa. In addition, viral particles bound high molecular weight proteins. Purified proteins identified were enolase, beta-adrenergic receptor kinase (beta-ARK, translation elongation factor EF-1 alpha/Tu, and cadherin.

  3. Aedes aegypti, Aedes albopictus, and dengue in Argentina: current knowledge and future directions

    Directory of Open Access Journals (Sweden)

    Darío Vezzani

    2008-02-01

    Full Text Available Since the reinfestation of South American countries by Ae. aegypti, dengue fever (DF and dengue hemorrhagic fever (DHF have become a major public health concern. The aim of this paper was to review the information related with Aedes vectors and dengue in Argentina since the reintroduction of Ae. aegypti in 1986. The geographic distribution of Ae. albopictus is restricted to the Northeast, and that of Ae. aegypti has expanded towards the South and the West in comparison with the records during the eradication campaign in the 1960s. Since 1998, 4,718 DF cases have been reported concentrated in the provinces of Salta, Formosa, Misiones, Jujuy and Corrientes. Despite the circulation of three dengue virus serotypes (DENV-1, -2 and -3 in the North of the country, DHF has not occurred until the present. The information published over the last two decades regarding mosquito abundance, temporal variations, habitat characteristics, competition, and chemical and biological control, was reviewed. Considering the available information, issues pending in Argentina are discussed. The presence of three DENV, the potential spread of Ae. albopictus, and the predicted climate change suggest that dengue situation will get worse in the region. Research efforts should be increased in the Northern provinces, where DHF is currently an actual risk.

  4. Fertility in Hill Korwas -- a primitive tribe of Madhya Pradesh.

    Science.gov (United States)

    Pandey, G D; Tiwary, R S

    1996-12-01

    This study examines fertility behavior among 604 eligible couples in Hill Korwa tribes in Madhya Pradesh state, India. Low fertility patterns are compared to those of neighboring Gonds and nontribals from rural Jabalpur. The Hill Korwa are a subtribe of the Korwa, who remained in the hills and dense forests. Over 60% live in three tehsils of Surguja district, including Ambikapur tehsil where the study was conducted. Data were obtained in March 1991. Eligible couples were those where both partners live together and the noncontracepting wife is under age 50 and nonmenopausal. Only 3% were literate. Female marriage age was about 15 years. The median age was 23.8 years. 92% lived below the poverty line. The average number of children ever born (CEB) per couple was 1.9, compared to 2.5 for the Gond and 2.9 for nontribal couples. The CEB in a reproductive lifetime was 2.9, compared to 5.3 for Gond women and 5.9 for nontribal women. Fecundity among Hill Korwa women was 66% lower at younger ages (16-17 years and 17-18 years), and the differences increased with an increase in age at marriage. Hill Korwas had a low female age at marriage, low literacy, low percentages engaged in agriculture, and higher percentages living above the poverty line.

  5. Digital signage sobre IP

    OpenAIRE

    Casademont Filella, Albert

    2012-01-01

    Projecte que versa sobre el disseny i la implementació d'un sistema de Digital Signage (Cartelleria Digital) sobre IP, creant un gestor que permeti controlar de forma remota tot un conjunt de petits dispositius connectats a pantalles que emeten continguts multimèdia com vídeos, imatges, feeds rss...

  6. Midwest nukes tumble, rock industry: Byron, Marble Hill, Zimmer

    International Nuclear Information System (INIS)

    Nogee, A.

    1984-01-01

    The nuclear industry is suffering from a lack of investor confidence because of cancelled projects and the unprecedented rejection of an operating license for Commonwealth Edison's Byron plant on grounds that the utility failed to meet quality assurance responsibilities. When plans to complete the Zimmer and Marble Hill nuclear plants were abandoned, Bechtel came forward with a financing plan that, while rejected for Zimmer and Marble Hill, could lead to future bailouts. Both Zimmer and Marble Hill plants could be partially converted to coal. The loss of investor confidence in nuclear plants is largely due to political pressures brought on by a combination of citizen intervenors, whistle blowers on construction sites and disagreements between participating utilities. A rise in stock prices followed the cancellation announcements and the lowered investment security ratings

  7. Laboratory Evaluation of a Novel Lethal Ovitrap for Control of Aedes aegypti

    Science.gov (United States)

    Aedes aegypti and Aedes albopictus are known to flourish in a variety of natural and residential habitats and are competent vectors of at least 22 different arboviruses including dengue, chikungunya, and zika. Their global distribution, anthropophilic nature, and vector competency make them species ...

  8. Systematic Review: Land Cover, Meteorological, and Socioeconomic Determinants of Aedes Mosquito Habitat for Risk Mapping

    Science.gov (United States)

    Asian tiger and yellow fever mosquitoes (Aedes albopictus and Ae. aegypti) are global nuisances and are competent vectors for viruses such as Chikungunya (CHIKV), Dengue (DV), and Zika (ZIKV). This review aims to analyze available spatiotemporal distribution models of Aedes mosqu...

  9. Ecological Factors Determining Abundance of Parasitic Mites on Aedes spp. Larvae

    Directory of Open Access Journals (Sweden)

    Nurhadi Eko Firmansyah

    2017-12-01

    Full Text Available Ability to infestation and abundance of parasitic mites in Aedes spp. larvae cannot be separated from the influence of various factors. Ecological factors have been suggested to play a role determine the presence of parasitic mites that under certain conditions become a key factor in determining the abundance of parasitic mites on Aedes spp. larvae. The aim of this study to determine the ecological factors affect the abundance of parasitic mites on Aedes spp. larvae in Bogor Regency. Capturing of Aedes spp. larvae was performed directly on the habitats found in indoor and outdoor. Capturing mites in the body of Aedes spp. larvae was performed using insect forceps. Ecological factors measured were dissolved oxygen (DO, pH, temperature, and total dissolved solid (TDS. The influence of ecological factors was analyzed using regression and correlation analysis. The result of mite identification has been obtained three species of mites that are Halacarus sp., Histiostoma sp., and Hydrozetes sp. The result indicated that total dissolved solid (TDS and temperature was the factors that determined the abundance of mites. The factors of pH, and dissolved oxygen (DO did not determine the abundance of parasitic mites of Aedes spp. larvae. The research result can be further developed as a new alternative to Dengue Hemorraghic Fever control and provide information on parasitic mites that infest Aedes spp. larvae. In addition, this results become an early step in controlling of Aedes spp. strategy platform by the parasitic mites.

  10. Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia.

    Science.gov (United States)

    Ishak, Intan H; Jaal, Zairi; Ranson, Hilary; Wondji, Charles S

    2015-03-25

    Knowledge on the extent, distribution and mechanisms of insecticide resistance is essential for successful insecticide-based dengue control interventions. Here, we report an extensive resistance profiling of the dengue vectors Aedes aegypti and Aedes albopictus across Malaysia and establish the contribution of knockdown resistance mechanism revealing significant contrast between both species. Aedes mosquitoes were collected from four states in Malaysia in 2010 using ovitraps and tested against six major insecticides using WHO bioassays. Knockdown resistance (kdr) was investigated in both species. A moderate resistance to temephos was detected from samples collected in 2010 in Penang, Kuala Lumpur, Johor Bharu and Kota Bharu (1.5 Malaysia but neither of these mutations were found in Ae. albopictus. Additionally, signatures of selection were detected on the Voltage-gated sodium channel gene in Ae. aegypti but not in Ae. albopictus. The presence of the 1534C allele was significantly associated with pyrethroid resistance and an additive effect to pyrethroid resistance was observed in individuals containing both kdr alleles. Findings from this study will help to design and implement successful insecticide-based interventions against Ae. aegypti and Ae. albopictus to improve dengue control across Malaysia.

  11. 2013 strategic petroleum reserve big hill well integrity grading report.

    Energy Technology Data Exchange (ETDEWEB)

    Lord, David L.; Roberts, Barry L.; Lord, Anna C. Snider; Bettin, Giorgia; Sobolik, Steven Ronald; Park, Byoung Yoon; Rudeen, David Keith; Eldredge, Lisa; Wynn, Karen; Checkai, Dean; Perry, James Thomas

    2014-02-01

    This report summarizes the work performed in developing a framework for the prioritization of cavern access wells for remediation and monitoring at the Big Hill Strategic Petroleum Reserve site. This framework was then applied to all 28 wells at the Big Hill site with each well receiving a grade for remediation and monitoring. Numerous factors affecting well integrity were incorporated into the grading framework including casing survey results, cavern pressure history, results from geomechanical simulations, and site geologic factors. The framework was developed in a way as to be applicable to all four of the Strategic Petroleum Reserve sites.

  12. Susceptibility of larvae of Aedes aegypti (Linnaeus (Diptera: Culicidae to entomopathogenic nematode Heterorhabditis bacteriophora (Poinar (Rhabditida: Heterorhabditidae

    Directory of Open Access Journals (Sweden)

    María L. PESCHIUTTA

    2014-01-01

    Full Text Available Aedes aegypti (Linnaeus (Diptera: Culicidae es vector de los agentes etiológicos de la fiebre amarilla y del dengue. Una alternativa al control químico de este vector es el uso de agentes biológicos. Los nematodos entomopatógenos son efectivos en el control de plagas. La infectividad y el ciclo de vida de un aislado argentino de Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae en larvas de A. aegypti se registró por primera vez bajo condiciones de laborato - rio. Para cada unidad experimental, 30 larvas de mosquito de segundo estadio fueron expuestas a 8 dosis del nematodo (0:1, 1:1, 5:1, 15:1, 100:1, 500:1, 750:1, 1500:1. Los juveniles infectivos (JIs utilizados fueron multiplicados sobre Galleria mellonella (Lepidoptera: Pyralidae. La continuidad infectiva de los JIs obtenidos de A. aegypti fue probada aplicándolos en una dosis de 100:1 sobre larvas del mosquito . Las tasas de mortalidad fueron de 0% a 84%. El número de nematodos desarrollados dentro de la larva de mosquito, la mortalidad larval y los nuevos JIs se incrementaron con el aumento de la dosis de nematodos. Los resultados indican que H. bacteriophora es capaz de infectar larvas de A. aegypti , se desarrolla y produce nuevos JIs, permitiendo la continuidad de su ciclo de vida.

  13. An updated checklist of species of Aedes and Verrallina of northeastern India.

    Science.gov (United States)

    Dutta, P; Khan, S A; Khan, A M; Sharma, C K; Mahanta, J

    2010-06-01

    A mosquito faunistic survey conducted during 1999-2005 in 7 different states of the northeastern (NE) region of India revealed that the region is very rich in mosquito fauna, particularly of the genus Aedes. From the present survey and earlier published reports, a total of 48 species of Aedes and a related genus Verrallina were recorded. The state of Assam contributed the highest number of species compared to the other 6 states, namely Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Nagaland, and Tripura. Larvae of 46 species were collected from 16 different habitats and for some species the habitats were species specific. The known dengue vectors, Aedes aegypti and Ae. albopictus, were recorded from all 7 states, although Ae. aegypti was not known earlier from Mizoram, Nagaland, and Tripura states. Aedes albotaeniatus was a new addition to the Aedes fauna of the region.

  14. The Subgenus Stegomyia of Aedes in the Afrotropical Region. 2. The Dendrophilus Group of Species (Diptera: Culicidae) (Contributions of the American Entomological Institute. Volume 29, Number 4, 1997)

    Science.gov (United States)

    1997-01-01

    or reservoirs of eight viruses , six of which cause human illness (Chikungunya, dengue 1 and 2, Dugbe, Rift Valley fever, yellow fever and Zika ...suggested that Ae. deboeri may be the jungle vector of the yellow fever virus in Langata. A edes Aedes Aedes Aedes (Stegomyia) demeilloni Edwards... Aedes (Stegomyia) Iedgeri (Diptera: Culicidae). Mosq. Syst. 13: 92-113. Aedes (Stegomyia) bromeliae (Diptera: Culicidae), the yellow fever virus

  15. Vector Competence of Aedes aegypti and Aedes polynesiensis Populations from French Polynesia for Chikungunya Virus.

    Directory of Open Access Journals (Sweden)

    Vaea Richard

    2016-05-01

    Full Text Available From October 2014 to March 2015, French Polynesia experienced for the first time a chikungunya outbreak. Two Aedes mosquitoes may have contributed to chikungunya virus (CHIKV transmission in French Polynesia: the worldwide distributed Ae. aegypti and the Polynesian islands-endemic Ae. polynesiensis mosquito.To investigate the vector competence of French Polynesian populations of Ae. aegypti and Ae. polynesiensis for CHIKV, mosquitoes were exposed per os at viral titers of 7 logs tissue culture infectious dose 50%. At 2, 6, 9, 14 and 21 days post-infection (dpi, saliva was collected from each mosquito and inoculated onto C6/36 mosquito cells to check for the presence of CHIKV infectious particles. Legs and body (thorax and abdomen of each mosquito were also collected at the different dpi and submitted separately to viral RNA extraction and CHIKV real-time RT-PCR.CHIKV infection rate, dissemination and transmission efficiencies ranged from 7-90%, 18-78% and 5-53% respectively for Ae. aegypti and from 39-41%, 3-17% and 0-14% respectively for Ae. polynesiensis, depending on the dpi. Infectious saliva was found as early as 2 dpi for Ae. aegypti and from 6 dpi for Ae. polynesiensis. Our laboratory results confirm that the French Polynesian population of Ae. aegypti is highly competent for CHIKV and they provide clear evidence for Ae. polynesiensis to act as an efficient CHIKV vector.As supported by our findings, the presence of two CHIKV competent vectors in French Polynesia certainly contributed to enabling this virus to quickly disseminate from the urban/peri-urban areas colonized by Ae. aegypti to the most remote atolls where Ae. polynesiensis is predominating. Ae. polynesiensis was probably involved in the recent chikungunya outbreaks in Samoa and the Cook Islands. Moreover, this vector may contribute to the risk for CHIKV to emerge in other Polynesian islands like Fiji, and more particularly Wallis where there is no Ae. aegypti.

  16. Larval mosquito habitat utilization and community dynamics of Aedes albopictus and Aedes japonicus (Diptera: Culicidae).

    Science.gov (United States)

    Bartlett-Healy, Kristen; Unlu, Isik; Obenauer, Peter; Hughes, Tony; Healy, Sean; Crepeau, Taryn; Farajollahi, Ary; Kesavaraju, Banu; Fonseca, Dina; Schoeler, George; Gaugler, Randy; Strickman, Daniel

    2012-07-01

    Aedes albopictus (Skuse) and Ae. japonicus (Theobald) are important container-inhabiting mosquitoes that transmit disease agents, outcompete native species, and continue to expand their range in the United States. Both species deposit eggs in natural and artificial containers and thrive in peridomestic environments. The goal of our study was to examine the types and characteristics of containers that are most productive for these species in the northeastern United States. In total, 306 containers were sampled in urban, suburban, and rural areas of New Jersey. Multiple biotic and abiotic factors were recorded in an attempt to identify variables associated with the productivity of each species. Based on pupal abundance and density of container types, results showed that tires, trash cans, and planter dishes were the most important containers for Ae. albopictus, while planter dishes were the most important containers for Ae. japonicus. Container color (black and gray), material (rubber), and type (tires) were correlated with species presence for Ae. albopictus and Ae. japonicus. These factors may play a role in the selection of oviposition sites by female mosquitoes or in the survival of their progeny. Differences in species composition and abundance were detected between areas classified as urban, suburban, and rural. In urban and suburban areas, Ae. albopictus was more abundant in container habitats than Ae. japonicus; however, Ae. japonicus was more abundant in rural areas, and when water temperatures were below 14 degrees C. Our results suggest many variables can influence the presence of Ae. albopictus and Ae. japonicus in container habitats in northeastern United States.

  17. Primeiro registro de Aedes (Stegomyia albopictus no Estado do Ceará, Brasil First report of Aedes (Stegomyia albopictus in the state of Ceará, Brazil

    Directory of Open Access Journals (Sweden)

    Víctor Emanuel Pessoa Martins

    2006-08-01

    Full Text Available Pela primeira vez é registrada a ocorrência de Aedes (Stegomyia albopictus em área urbana da cidade de Fortaleza, no Estado do Ceará, Brasil. De janeiro a julho de 2005 foram utilizadas ovitrampas para a coleta de ovos de Aedes spp., os quais foram mantidos em laboratório para desenvolvimento até a fase adulta. Os mosquitos resultantes foram identificados e submetidos a testes para o isolamento dos vírus da dengue. Foram identificados 13 espécimes de Aedes albopictus, todos fêmeas. Não foi isolado vírus da dengue em nenhum dos pools de mosquitos. Apesar de o Aedes albopictus não ter sido incriminado por surtos de dengue no Brasil, não se pode descartar a possibilidade da transmissão dos vírus da dengue por tais mosquitos.For the first time, the occurrence of Aedes (Stegomyia albopictus in an urban area of the city of Fortaleza, Northeastern, Brazil, is reported. From January to July 2005, ovitraps were used to collect eggs from Aedes spp., which were kept under laboratory conditions to develop into the adult phase. The resultant mosquitoes were identified and subjected to dengue virus isolation tests. Thirteen specimens of Aedes albopictus, all females, were identified. No dengue virus was isolated in any of the mosquito pools. Even though Aedes albopictus has not been incriminated in Brazilian dengue outbreaks, the possibility of dengue virus transmission by these mosquitoes cannot be dismissed.

  18. Apuntes sobre esporotricosis

    OpenAIRE

    Londoño, Fabio

    2011-01-01

    Se hace una breve reseña histórica de las primeras comunicaciones sobre esporotricosis. Se destaca la amplia difusión de la enfermedad en nuestro país. 3. Se hace notar la no existencia de datos bibliográficos sobre formas extracutáneas. 4. Se llama la atención sobre la eficaz ayuda de la intradermo-reacción con esporotriquina en el diagnóstico de la enfermedad. 5- Se comunica un caso de esporotricosis verrugosa tratado con anfotericin B, con excelentes resultados

  19. Apuntes sobre esporotricosis

    Directory of Open Access Journals (Sweden)

    Fabio Londoño

    1960-01-01

    Full Text Available Se hace una breve reseña histórica de las primeras comunicaciones sobre esporotricosis. Se destaca la amplia difusión de la enfermedad en nuestro país. 3. Se hace notar la no existencia de datos bibliográficos sobre formas extracutáneas. 4. Se llama la atención sobre la eficaz ayuda de la intradermo-reacción con esporotriquina en el diagnóstico de la enfermedad. 5- Se comunica un caso de esporotricosis verrugosa tratado con anfotericin B, con excelentes resultados

  20. Eagle Hill, Kenya: changes over 60 years | Thomsett | Scopus ...

    African Journals Online (AJOL)

    Eagle Hill, the study site of the late Leslie Brown, was first surveyed over 60 years ago in 1948. The demise of its eagle population was near-complete less than 50 years later, but significantly, the majority of these losses occurred in the space of a few years in the late 1970s. Unfortunately, human densities and land use ...

  1. 78 FR 76100 - Black Hills National Forest Advisory Board

    Science.gov (United States)

    2013-12-16

    ...: The Black Hills National Forest Advisory Board (Board) will meet in Rapid City, South Dakota. The... Ranger District, 8221 South Highway 16, Rapid City, South Dakota. Written comments may be submitted as... the public. The agenda will include time for people to make oral statements of three minutes or less...

  2. Correct thermodynamic forces in Tsallis thermodynamics: connection with Hill nanothermodynamics

    International Nuclear Information System (INIS)

    Garcia-Morales, Vladimir; Cervera, Javier; Pellicer, Julio

    2005-01-01

    The equivalence between Tsallis thermodynamics and Hill's nanothermodynamics is established. The correct thermodynamic forces in Tsallis thermodynamics are pointed out. Through this connection we also find a general expression for the entropic index q which we illustrate with two physical examples, allowing in both cases to relate q to the underlying dynamics of the Hamiltonian systems

  3. Key tiger habitats in the Garo Hills of Meghalaya

    Science.gov (United States)

    Ashish Kumar; Bruce G. Marcot

    2010-01-01

    We describe assumed tiger habitat characteristics and attempt to identify potential tiger habitats in the Garo Hills region of Meghalaya, North East India. Conserving large forest tracts and protected wildlife habitats provides an opportunity for restoring populations of wide-ranging wildlife such as tigers and elephants. Based on limited field observations coupled...

  4. Some noteworthy distributional records from the Gwassi Hills area ...

    African Journals Online (AJOL)

    A preliminary inventory of birds occurring in the Gwassi Hills area was compiled by Bradley et .... Woodpecker by some), on the basis of a paler grey head and underparts, and the absence of .... Bay, 50 km to the northeast (Lewis & Pomeroy 1989, D. Turner pers. ... These birds appeared slightly darker with more slaty tones.

  5. State of conservation of Santa Martha's hills, Colombia

    International Nuclear Information System (INIS)

    Lopez Salgado, Hector Jaime; Carbono Delahoz, Eduino

    2006-01-01

    The urban growth of Santa Marta and the occupation without planning of their surrounding hills, affect their natural conditions. The hills are part of the scenic beauty of the city and its conservation is indispensable to maintain the standard of life in her the vegetal cover corresponds to forests and scrubs of isomegathermic floor with xeromorphic characters, due to the low pluvial precipitation, this landscape characterizes the Colombian Caribbean coast in a narrow strip that extends from the south of the Guajira to the gulf of Morrosquillo. At the present time, only they are left surpluses in natural state, due to the floristic simplification of the vegetation, the introduction of invading species and modification of the landscape. The responsibility of the suitable handling of the urban space is imperative for the official, private sector and citizen institutions. The objective is to make an evaluation of the environmental conditions of hills of the city, in order to obtain basic elements, for the design of appropriate strategies of handling and its conservation. By means of the use of the GIS, with verification and aerial photo interpretation of field a multi-temporary study (1955-1993-2003) of the vegetal cover of the hills was elaborated that allowed to establish the happened changes. The execution of inventors allowed knowing the wealth and the structure floristic the vegetal cover of the area. The work was executed between May of the 2004 and May of the 2005

  6. Dental disease control in Pine Hill, New Mexico.

    Science.gov (United States)

    Carberry, Frank J; Cloud, Bill; Finster, Carolyn

    2004-02-01

    One-year results of a community-operated dental disease control project in Pine Hill, New Mexico. The program uses fluoride, chiefly rinse, and has not only reduced the amount of decay in permanent teeth, but has markedly reduced the need for restorative care of primary teeth.

  7. Amphibian diversity in Shimba Hills National Reserve, Kenya: A ...

    African Journals Online (AJOL)

    We present the first annotated amphibian checklist of Shimba Hills National Reserve (SHNR). The list comprises of 30 currently known amphibians (28 anurans and two caecilians), which includes 11 families and 15 genera. In addition, individual records per species, distribution in the reserve and brief remarks about the ...

  8. Measurements of the near-surface flow over a hill

    Science.gov (United States)

    Vosper, S. B.; Mobbs, S. D.; Gardiner, B. A.

    2002-10-01

    The near-surface flow over a hill with moderate slope and height comparable with the boundary-layer depth is investigated through field measurements of the mean flow (at 2 m), surface pressure, and turbulent momentum flux divergence between 8 and 15 m. The measurements were made along an east-west transect across the hill Tighvein (height 458 m, approximate width 8 km) on the Isle of Arran, south-west Scotland, during two separate periods, each of around three-weeks duration. Radiosonde ascents are used to determine the variation of a Froude number, FL = U/NL, where U is the wind speed at the middle-layer height, hm, N is the mean Brunt-Väisälä frequency below this height and L is a hill length-scale. Measurements show that for moderately stratified flows (for which FL 0.25) a minimum in the hill-induced surface-pressure perturbation occurs across the summit and this is accompanied by a maximum in the near-surface wind speed. In the more strongly stratified case (FL 0.25) the pressure field is more asymmetric and the lee-slope flow is generally stronger than on the windward slope. Such a flow pattern is qualitatively consistent with that predicted by stratified linear boundary-layer and gravity-wave theories. The near-surface momentum budget is analysed by evaluating the dominant terms in a Bernoulli equation suitable for turbulent flow. Measurements during periods of westerly flow are used to evaluate the dominant terms, and the equation is shown to hold to a reasonable approximation on the upwind slope of the hill and also on the downwind slope, away from the summit. Immediately downwind of the summit, however, the Bernoulli equation does not hold. Possible reasons for this, such as non-separated sheltering and flow separation, are discussed.

  9. Phosphorus forms in soils of Oban Hills, Akamkpa, Cross River State ...

    African Journals Online (AJOL)

    Oban Hills is located at Akamkpa in the Southern Senatorial District of Cross River, State, Nigeria. Phosphorus (P)-rich soil from the Hills is expected to have an effect on retention and distribution in the highly acidic soils surrounding the area inundated for several years. Phosphorus forms in the soils of the Hills varied with ...

  10. Multi-scale habitat use of male ruffed grouse in the Black Hills National Forest

    Science.gov (United States)

    Cassandra L. Mehls; Kent C. Jensen; Mark A. Rumble; Michael C. Wimberly

    2014-01-01

    Ruffed grouse (Bonasa umbellus) are native upland game birds and a management indicator species (MIS) for aspen (Populus tremuloides) in the Black Hills National Forest (Black Hills). Our objective was to assess resource selection of male ruffed grouse to identify the most appropriate scale to manage for aspen and ruffed grouse in the Black Hills. During spring 2007...

  11. Evaluation of a sticky trap (AedesTraP, made from disposable plastic bottles, as a monitoring tool for Aedes aegypti populations

    Directory of Open Access Journals (Sweden)

    de Santos Eloína Maria

    2012-09-01

    Full Text Available Abstract Background Dengue virus, which is transmitted by Aedes aegypti mosquitoes is the most important emerging viral disease, infecting more than 50 million people annually. Currently used sticky traps are useful tools for monitoring and control of A. aegypti, despite differences in efficiency, labor requirements and cost. In the present work, a field assay was carried out to evaluate the performance of a sticky trap (AedesTrap, produced using disposable material, in capturing gravid Aedes spp. females. Additionally, conditions necessary for the improved performance of the device, such as number of traps per site and location (indoors or outdoors were evaluated. Methods During a one year period, traps were placed in a dengue endemic area in 28 day cycles. The trap, named AedesTrap, consisted of a disposable plastic soda bottle coated inside with colophony resin, which served as a sticky substrate. Disposable bottles were donated by restaurants, and traps were made by laboratory staff, reducing the cost of the sticky trap (less than U$3. Mosquito capture in indoor and outdoor areas was compared by placing the traps in laundry room, kitchen or bedroom (indoors and front or back yard (outdoors. The relationship between the number of AedesTraps and quantity of captured mosquitoes was investigated by utilizing one or three traps/site. Results During a 28 day cycle, a single AedesTrap was capable of capturing up to 15 A. aegypti in a house, with a mean capture of 0.5 to 2.63 females per premise. The AedesTrap collected three times more outdoors versus indoors. Similarly, the capability of detecting Aedes spp. infestation, and of capturing females, was three times higher when using three AedesTraps per house, compared with one trap per house. Conclusions AedesTrap was shown to be capable of capturing A. aegypti and other culicidae, providing information on the adult mosquito population, and allowing the identification of areas critically

  12. Evaluation of a sticky trap (AedesTraP), made from disposable plastic bottles, as a monitoring tool for Aedes aegypti populations.

    Science.gov (United States)

    de Santos, Eloína Maria Mendonça; de Melo-Santos, Maria Alice Varjal; de Oliveira, Claudia Maria Fontes; Correia, Juliana Cavalcanti; de Albuquerque, Cleide Maria Ribeiro

    2012-09-07

    Dengue virus, which is transmitted by Aedes aegypti mosquitoes is the most important emerging viral disease, infecting more than 50 million people annually. Currently used sticky traps are useful tools for monitoring and control of A. aegypti, despite differences in efficiency, labor requirements and cost. In the present work, a field assay was carried out to evaluate the performance of a sticky trap (AedesTrap), produced using disposable material, in capturing gravid Aedes spp. females. Additionally, conditions necessary for the improved performance of the device, such as number of traps per site and location (indoors or outdoors) were evaluated. During a one year period, traps were placed in a dengue endemic area in 28 day cycles. The trap, named AedesTrap, consisted of a disposable plastic soda bottle coated inside with colophony resin, which served as a sticky substrate. Disposable bottles were donated by restaurants, and traps were made by laboratory staff, reducing the cost of the sticky trap (less than U$3). Mosquito capture in indoor and outdoor areas was compared by placing the traps in laundry room, kitchen or bedroom (indoors) and front or back yard (outdoors). The relationship between the number of AedesTraps and quantity of captured mosquitoes was investigated by utilizing one or three traps/site. During a 28 day cycle, a single AedesTrap was capable of capturing up to 15 A. aegypti in a house, with a mean capture of 0.5 to 2.63 females per premise. The AedesTrap collected three times more outdoors versus indoors. Similarly, the capability of detecting Aedes spp. infestation, and of capturing females, was three times higher when using three AedesTraps per house, compared with one trap per house. AedesTrap was shown to be capable of capturing A. aegypti and other culicidae, providing information on the adult mosquito population, and allowing the identification of areas critically infested by mosquitoes. Low requirements for skilled labor

  13. Especificidade da armadilha Adultrap para capturar fêmeas de Aedes aegypti (Diptera: Culicidae Specificity of the Adultrap for capturing females of Aedes aegypti (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Almério de Castro Gomes

    2007-04-01

    Full Text Available A Adultrap é uma nova armadilha feita para capturar fêmeas de Aedes aegypti. Foram realizados testes para avaliar sua especificidade tendo como referência a técnica da aspiração da espécie em abrigos artificiais. A Adultrap ficou exposta por 24 horas no intradomicílio e peridomicílio de 120 casas sorteadas em dois bairros da Cidade de Foz do Iguaçu, Estado do Paraná. O teste estatístico foi o modelo log-linear de Poisson. O resultado foi a captura de 726 mosquitos Culicidae, dos quais 80 eram Aedes aegypti. A Adultrap capturou apenas fêmeas desta espécie, enquanto o aspirador os dois sexos de Aedes aegypti e mais cinco outras espécies. A Adultrap capturou Aedes aegypti dentro e fora das casas, mas a análise indicou que no peridomicílio a armadilha capturou significantemente mais fêmeas do que a aspiração. Também, ficou evidenciada a sensibilidade da Adultrap para detectar Aedes aegypti em situação de baixa freqüência.The Adultrap is a new trap built for capturing females of Aedes aegypti. Tests were carried out to evaluate the specificity of this trap in comparison with the technique of aspiration of specimens in artificial shelters. Adultraps were kept for 24 hours inside and outside 120 randomly selected homes in two districts of the city of Foz do Iguaçú, State of Paraná. The statistical test was Poisson’s log-linear model. The result was 726 mosquitoes captured, of which 80 were Aedes aegypti. The Adultrap captured only females of this species, while the aspiration method captured both sexes of Aedes aegypti and another five species. The Adultrap captured Aedes aegypti inside and outside the homes, but the analysis indicated that, outside the homes, this trap captured significantly more females than aspiration did. The sensitivity of the Adultrap for detecting females of Aedes aegypti in low-frequency situations was also demonstrated.

  14. Actividad larvicida de la toronja, Citrus paradisi (Rutaceae sobre dos vectores del dengue

    Directory of Open Access Journals (Sweden)

    Jorge Morales-Saldaña

    2013-04-01

    Full Text Available La actividad larvicida del aceite esencial de la toronja (Citrus paradisi fue evaluada contra los mosquitos Aedes aegypti y Aedes albopictus, los cuales representan vectores potenciales de la fiebre del dengue. La CL50 del aceite esencial de la toronja fue de 47,3 ppm y 85,1 ppm para Ae. aegypti y Ae. albopictus, respectivamente. El análisis del aceite por GC-MS, mostró químicos conocidos como insecticidas los cuales podría explicar la acción tóxica sobre las larvas de los mosquitos evaluados. Esta actividad tóxica sobre Ae. aegypti fue mayor en comparación con otros aceites cítricos, lo que sugiere mayores instigaciones en esta línea y de esta manera poder recomendarlo como regulador de mosquitos a las diferentes campañas contra el Ae. aeygpti. En la literatura revisada no encontramos ninguna investigación donde haya sido evaluado aceites esenciales de cítricos contra larvas del mosquito Ae. albopictus.

  15. Identification of Essential Containers for Aedes Larval Breeding to Control Dengue in Dhaka, Bangladesh.

    Science.gov (United States)

    Ferdousi, Farhana; Yoshimatsu, Shoji; Ma, Enbo; Sohel, Nazmul; Wagatsuma, Yukiko

    2015-12-01

    Dengue fever (DF), one of the most important emerging arboviral diseases, is transmitted through the bite of container breeding mosquitoes Aedes aegypti and Aedes albopictus. A household entomological survey was conducted in Dhaka from August through October 2000 to inspect water-holding containers in indoor, outdoor, and rooftop locations for Aedes larvae. The objective of this study was to determine mosquito productivity of each container type and to identify some risk factors of households infested with Aedes larvae. Of 9,222 households inspected, 1,306 (14.2%) were positive for Aedes larvae. Of 38,777 wet containers examined, 2,272 (5.8%) were infested with Aedes larvae. Containers used to hold water, such as earthen jars, tanks, and drums were the most common containers for larval breeding. Tires in outdoor and rooftop locations of the households were also important for larval breeding. Although present in abundance, buckets were of less importance. Factors such as independent household, presence of a water storage system in the house, and fully/partly shaded outdoors were found to be significantly associated with household infestation of Aedes larvae. Identification and subsequent elimination of the most productive containers in a given area may potentially reduce mosquito density to below a level at which dengue transmission may be halted.

  16. The immune strategies of mosquito Aedes aegypti against microbial infection.

    Science.gov (United States)

    Wang, Yan-Hong; Chang, Meng-Meng; Wang, Xue-Li; Zheng, Ai-Hua; Zou, Zhen

    2018-06-01

    Yellow fever mosquito Aedes aegypti transmits many devastating arthropod-borne viruses (arboviruses), such as dengue virus, yellow fever virus, Chikungunya virus, and Zika virus, which cause great concern to human health. Mosquito control is an effective method to block the spread of infectious diseases. Ae. aegypti uses its innate immune system to fight against arboviruses, parasites, and fungi. In this review, we briefly summarize the recent findings in the immune response of Ae. aegypti against arboviral and entomopathogenic infections. This review enriches our understanding of the mosquito immune system and provides evidence to support the development of novel mosquito control strategies. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Larvicidal activity of some Cerrado plant extracts against Aedes aegypti

    OpenAIRE

    Rodrigues, A.M.S.; De Paula, J.E.; Dégallier, Nicolas; Molez, Jean-François; Espindola, L.S.

    2006-01-01

    One hundred ninety hexanic and ethanolic extracts from 27 plant species from the Cerrado biome of Brazil were tested for larvicidal activity against 3rd-stage Aedes aegypti larvae at 500 mu g/ml. Fourteen extracts from 7 species showed activity (> 65% mortality) against the larvae. Of these, Duguetia furfuracea, Piptocarpha rotundifolia, Casearia sylvestris var. lingua, Serjania lethalis, and Xylopia aromatica were active at 56.6, 162.31, 232.4, 285.76, and 384.37 mu g/ml, respectively. Annon...

  18. History of domestication and spread of Aedes aegypti - A Review

    Directory of Open Access Journals (Sweden)

    Jeffrey R Powell

    2013-01-01

    Full Text Available The adaptation of insect vectors of human diseases to breed in human habitats (domestication is one of the most important phenomena in medical entomology. Considerable data are available on the vector mosquito Aedes aegypti in this regard and here we integrate the available information including genetics, behaviour, morphology, ecology and biogeography of the mosquito, with human history. We emphasise the tremendous amount of variation possessed by Ae. aegypti for virtually all traits considered. Typological thinking needs to be abandoned to reach a realistic and comprehensive understanding of this important vector of yellow fever, dengue and Chikungunya.

  19. Aedes aegypti Larvicidal Sesquiterpene Alkaloids from Maytenus oblongata.

    Science.gov (United States)

    Touré, Seindé; Nirma, Charlotte; Falkowski, Michael; Dusfour, Isabelle; Boulogne, Isabelle; Jahn-Oyac, Arnaud; Coke, Maïra; Azam, Didier; Girod, Romain; Moriou, Céline; Odonne, Guillaume; Stien, Didier; Houël, Emeline; Eparvier, Véronique

    2017-02-24

    Four new sesquiterpene alkaloids (1-4) with a β-dihydroagrofuran skeleton and a new triterpenoid (5) were isolated from an ethyl acetate extract of Maytenus oblongata stems. Their structures were elucidated using 1D and 2D NMR spectroscopy as well as MS and ECD experiments. The M. oblongata stem EtOAc extract and the pure compounds isolated were tested for larvicidal activity against Aedes aegypti under laboratory conditions, and compounds 2 and 3 were found to be active.

  20. Aktivitas Beberapa Atraktan Pada Perangkap Telur Berperekat Terhadap Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Milana Salim

    2017-02-01

    Full Text Available AbstractControl of Aedes aegypti mosquito as dengue haemorrhagic fever/DHF vector can be conducted using the ovitrap modified into a sticky ovitrap. The addition of attractant substances to the ovitrap can attract more mosquitoes comes to the trap, and prevent mosquitoes laying eggs in other places. The aim of this research was to compare hay infusion water and larva rearing water as attractant which combined with sugar-apple (Annona squamosa seed extract by counting the mosquitoes and eggs trapped. This research used six types medium: hay infusion water, larva rearing water, hay infusion water + sugar-apple seed extract, larva rearing water + sugar-apple seed extract, aquadest + sugar-apple seed extract, and aquadest only as a control. Sample used were 25 gravid female of Ae. aegypti mosquitoes with five replications. Mosquitoes and eggs which trapped were counted. This research showed that the number of mosquito trapped and eggs hatched more found in sticky ovitrap with hay infusion water. Statistic analyzed by ANOVA showed that there is no significant difference towards number off mosquito trapped in sticky ovitrap (p>0,05 whereas the medium material has significant difference towards number off egg hatched than others (p<0,05.Keywords: Sticky ovitrap, attractant, Aedes aegypti AbstrakPengendalian nyamuk Aedes aegypti sebagai vektor demam berdarah dengue/DBD dapat dilakukan menggunakan ovitrap yang dimodifikasi dengan perekat menjadi sticky ovitrap. Penambahan atraktan pada ovitrap dapat menarik lebih banyak nyamuk datang ke perangkap yang dipasang dan mencegah nyamuk bertelur di tempat lain. Tujuan penelitian ini adalah untuk mengetahui aktivitas atraktan air rendaman jerami dan air bekas kolonisasi yang dikombinasikan dengan ekstrak biji srikaya pada sticky ovitrap terhadap jumlah nyamuk dan telur yang ditemukan. Enam jenis media uji digunakan dalam penelitian ini yaitu air rendaman jerami, air bekas kolonisasi nyamuk, air rendaman jerami

  1. [Potential of Bacillus thuringiensis israelensis Berliner for controlling Aedes aegypti].

    Science.gov (United States)

    Polanczyk, Ricardo Antonio; Garcia, Marcelo de Oliveira; Alves, Sérgio Batista

    2003-12-01

    The importance of the entomopathogenic bacterium Bacillus thuringiensis israelensis in the control of Aedes aegypti is presented. The use and potential of B. thuringiensis israelensis against the mosquito vector of dengue fever is described. Other aspects such as insect's resistance development against chemicals and advantages and constraints of using microbial control are discussed. Emphasis is given to the importance of the use of this bacterium in Brazil, which could contribute significantly to solving the mosquito problem without affecting the environment, humans and others invertebrate organisms in critical regions.

  2. Distribution and larval breeding habitats of Aedes mosquito species in residential areas of northwest Ethiopia.

    Science.gov (United States)

    Ferede, Getachew; Tiruneh, Moges; Abate, Ebba; Kassa, Wondmeneh Jemberie; Wondimeneh, Yitayih; Damtie, Demekech; Tessema, Belay

    2018-01-01

    The Aedes mosquito is a vector for transmitting many arboviruses. Knowledge of the breeding habitat of this vector is vital for implementing appropriate interventions. Thus, this study was conducted to determine the breeding habitats and presence of Aedes mosquito species in the study areas. A house-to-house cross-sectional survey of Aedes mosquito breeding habitats was carried out in Metema and Humera, Ethiopia, in August 2017. All available water-holding containers present in and around houses were inspected for the presence of immature stages of Aedes mosquitoes, and they were collected and reared to the adult stage for species identification. In the larval survey, the house index, container index, and Breteau index were computed as risk indices. Of the 384 houses surveyed for the presence of Aedes mosquito larval breeding, 98 were found to be positive for larvae. During the survey, a total of 566 containers were inspected, of which 186 were found to be infested with Aedes mosquito larvae, with a container index of 32.9, a house index of 25.5, and a Breteau index of 48.4. The most common Aedes mosquito breeding habitats were discarded tires (57.5%), followed by mud pots (30.0%). Of the 1,077 larvae and pupae collected and reared, Aedes aegypti (49.3%), Ae. vittatus (6.5%), and Culex species (44.2%) were identified. Discarded tires were the most preferred breeding habitats for Aedes mosquitoes. Moreover, Ae. aegypti , the main vector of dengue and other arboviruses, was identified for the first time in this region, suggesting a high potential for arbovirus transmission in the study areas.

  3. Spinosad as an effective larvicide for control of Aedes albopictus and Aedes aegypti, vectors of dengue in southern Mexico.

    Science.gov (United States)

    Marina, Carlos F; Bond, J Guillermo; Casas, Mauricio; Muñoz, José; Orozco, Arnoldo; Valle, Javier; Williams, Trevor

    2011-01-01

    Field trials were conducted during the wet and dry seasons in periurban and semi-rural cemeteries in southern Mexico to determine the efficacy of a suspension concentrate formulation of spinosad (Tracer 480SC) on the inhibition of development of Aedes albopictus L. and Ae. aegypti Skuse. For this, oviposition traps were treated with spinosad (1 or 5 mg L(-1)), Bacillus thuringiensis israelensis (Bti, VectoBac 12AS), a sustained release formulation of temephos and a water control. Ae. albopictus was subordinate to Ae. aegypti during the dry season, but became dominant or codominant during the wet season at both sites. The two species could not be differentiated in field counts on oviposition traps. Mean numbers of larvae + pupae of Aedes spp. in Bti-treated containers were similar to the control at both sites during both seasons. The duration of complete absence of aquatic stages varied from 5 to 13 weeks for the spinosad treatments and from 6 to 9 weeks for the temephos treatment, depending on site, season and product concentration. Predatory Toxorhynchites theobaldi Dyar and Knab suffered low mortality in control and Bti treatments, but high mortality in spinosad and temephos treatments. Egg counts and percentage of egg hatch of Aedes spp. increased significantly between the dry and wet seasons, but significant treatment differences were not detected. Temephos granules and a suspension concentrate formulation of spinosad were both highly effective larvicides against Ae. aegypti and Ae. albopictus. These compounds merit detailed evaluation for inclusion in integrated control programs targeted at Ae. aegypti and Ae. albopictus in regions where they represent important vectors of human diseases. Copyright © 2010 Society of Chemical Industry.

  4. [Infestation status Aedes albopictus and related mosquito-borne infectious disease risk in central urban area in Shanghai].

    Science.gov (United States)

    Gao, Q; Xiong, C L; Zhou, Y B; Cao, H; Jiang, Q W

    2016-05-01

    To evaluate Aedes albopictus infestation status in the central urban area of Shanghai, and analyze the related epidemic risk of mosquito-borne infectious disease. Consecutive mosquito surveillance was conducted in the green lands and residential areas in the central urban area of Shanghai during 2012-2014, the Aedes albopictus density and its seasonal fluctuation were observed; the sequence of Aedes albopictus in Shanghai was aligned with that in other epidemic area abroad, and the susceptibility of Aedes albopictus to mosquito-borne virus and endemic risk were analyzed. No Aedes aegypti was found in the central urban area of Shanghai. As predominant species in both the residential area and the green lands, the proportion of Aedes albopictus in the residential area was significantly higher than that in the green lands(78.53% vs. 19.99%, χ(2) =15 525.168, PAedes albopictus in Shanghai and Aedes albopictus in Africa was quite far. No Aedes aegypti was found in Shanghai and its surrounding areas, while Aedes albopictus infestation in the central urban area of Shanghai was serious. Strict measures should be taken to reduce the Aedes albopictus density for the effective control Zika virus spread.

  5. A language for image processing HILLS and its supporting system SDIP

    International Nuclear Information System (INIS)

    Suzuki, H.; Toriwaki, J.

    1984-01-01

    This paper presents a language HILLS and its supporting system SDIP for image processing. HILLS is a key-word type language for describing image processing procedures by using subroutine packages SLIP and SPIDER. SDIP, written in FORTRAN to keep portability, supports programming by HILLS in interactive mode including functions such as editing, translating HILLS into FORTRAN, error detection, and providing manual information. Results of preliminary experiments suggest that HILLS and SDIP are very useful tools for beginners and researchers in application fields of image processing to develop desired image analysis procedures

  6. Actividades de la brigada de control de focos del Aedes aegypti: evaluación de su calidad en un área de salud

    Directory of Open Access Journals (Sweden)

    Meinardo Zayas Vinent

    2014-01-01

    Full Text Available Se efectuó una investigación sobre sistemas y servicios de salud, con vistas a evaluar la calidad de las actividades de la brigada de control de focos del Aedes aegypti en el Policlínico "Dr. Carlos Juan Finlay" de Santiago de Cuba, durante el mes de Junio del 2013, para lo cual se diseñó un sistema de indicadores de estructura, procesos, resultados e impacto, con una evaluación final integradora que favoreció la valoración de los objetivos de trabajo de esta brigada en la vigilancia y lucha antivectorial. Todos los indicadores fueron evaluados de bien y se propuso este instrumento para ser aplicado por los diferentes niveles de actuación en la lucha antivectorial

  7. EMG activities and plantar pressures during ski jumping take-off on three different sized hills.

    Science.gov (United States)

    Virmavirta, M; Perttunen, J; Komi, P V

    2001-04-01

    Different profiles of ski jumping hills have been assumed to make the initiation of take-off difficult especially when moving from one hill to another. Neuromuscular adaptation of ski jumpers to the different jumping hills was examined by measuring muscle activation and plantar pressure of the primary take-off muscles on three different sized hills. Two young ski jumpers volunteered as subjects and they performed several trials from each hill (K-35 m, K-65 m and K-90 m) with the same electromyographic (EMG) electrode and insole pressure transducer set-up. The results showed that the differences in plantar pressure and EMGs between the jumping hills were smaller than expected for both jumpers. The small changes in EMG amplitudes between the hills support the assumption that the take-off was performed with the same intensity on different jumping hills and the timing of the gluteus EMG demonstrates well the similarity of the muscle activation on different hills. On the basis of the results obtained it seems that ski jumping training on small hills does not disturb the movement patterns for bigger hills and can also be helpful for special take-off training with low speed.

  8. Horizontal gene transfer between Wolbachia and the mosquito Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Walker Thomas

    2009-01-01

    Full Text Available Abstract Background The evolutionary importance of horizontal gene transfer (HGT from Wolbachia endosymbiotic bacteria to their eukaryotic hosts is a topic of considerable interest and debate. Recent transfers of genome fragments from Wolbachia into insect chromosomes have been reported, but it has been argued that these fragments may be on an evolutionary trajectory to degradation and loss. Results We have discovered a case of HGT, involving two adjacent genes, between the genomes of Wolbachia and the currently Wolbachia-uninfected mosquito Aedes aegypti, an important human disease vector. The lower level of sequence identity between Wolbachia and insect, the transcription of all the genes involved, and the fact that we have identified homologs of the two genes in another Aedes species (Ae. mascarensis, suggest that these genes are being expressed after an extended evolutionary period since horizontal transfer, and therefore that the transfer has functional significance. The association of these genes with Wolbachia prophage regions also provides a mechanism for the transfer. Conclusion The data support the argument that HGT between Wolbachia endosymbiotic bacteria and their hosts has produced evolutionary innovation.

  9. Efficacy of topical permethrin as repellent against Aedes aegypti's bites.

    Science.gov (United States)

    Miot, Hélio Amante; Ferreira, Daniela Pinho; Mendes, Fabiana Guandalini; Carrenho, Flávia Roberta Hernandes; de Oliveira Amui, Isabela; Carneiro, Carlos Augusto Sá; Madeira, Newton Goulart

    2008-07-15

    Mosquitoes are the most important vectors of infectious diseases and their bites are related to several adverse skin reactions. Permethrin impregnated clothes are an efficient strategy against arthropods' bites; however, its topical efficacy as a repellent has not been well established. We studied the response to permethrin lotion 5 percent and N,N-Diethyl-meta-toluamide (DEET) spray 50 percent applied to the unprotected forearms of 10 volunteers. Each arm was exposed to 20 female mosquitoes of Aedes aegypti. We performed 71 bilateral comparative measurements evaluating the timing for the first bites. The average times for the arm without the product, with permethrin 5 percent, and with DEET 50 percent were: 7.9 seconds, 336.2 seconds and 7512.1 seconds. The results showed a significant difference between repellency times between either product and unprotected controls. In addition, there was a significant difference in time to first bite between permethrin and DEET treated arms (pAedes aegypti bites in this experimental setting. However, permethrin's profile of repellency was significantly inferior to that of DEET.

  10. Larvicidal Activity of Citrus Limonoids against Aedes albopictus Larvae

    Directory of Open Access Journals (Sweden)

    Hazrat Bilal

    2012-12-01

    Full Text Available Background: Development of insecticide resistance occurred due to the continuous and misuse of synthetic insecticidestherefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to suppressmosquito larval population.Methods: WHO recommended mosquito larval bioassay method for insecticide was used while for the analysis of citrus oils for limonin and nomilin content HPLC was used.Results: Among the two citrus cultivars tested as larvicide against Aedes albopictus, valencia late (Citrus sinensis wasthe best in terms of LC50 (297 ppm, % mortality (97% and LT50 (18.49 hours then freutrall early (Citrus reticulatewith LC50 (377.4 ppm, % mortality (88% and LT50 (31 hours, While nomilin gave lowest LC50 (121.04 ppm than limonin (382.22 ppm after 72 hours of exposure. Valencia late also had more limonin and nomilin (377 μg/ml and 21.19 μg/ml than freutrall early (5.29 μg/ml and 3.89 μg/ml respectively.Conclusion: Valencia late showed best results in term of LC50, LT50 and percentage mortality against Aedes albopictus as it has more amount of nomilin then freutrall early, however further evaluation in the field conditions is required.

  11. Profiles in medical courage: causation and Austin Bradford Hill

    Directory of Open Access Journals (Sweden)

    Robbins RA

    2012-09-01

    Full Text Available Austin Bradford Hill was a British epidemiologist and statistician who is best remembered for two landmark pulmonary studies. He was the statistician on the Medical Research Council Streptomycin in Tuberculosis Trial. This is regarded as the first randomized clinical trial. The second was the demonstration of the connection between cigarette smoking and lung cancer. However, Hill’s most lasting contribution may be his establishment of a group of conditions necessary to provide adequate evidence of a causal relationship between an incidence and a consequence, widely known as the Bradford Hill Criteria of Causation. In this profile of medical courage we examine his remarkable background that led to the epidemiological equivalent of Koch’s postulates.

  12. Environmental protection and regulatory compliance at the Elk Hills field

    International Nuclear Information System (INIS)

    Chappelle, H.H.; Donahoe, R.L.; Kato, T.T.; Ordway, H.E.

    1991-01-01

    Environmental protection has played an integral role in the development and operation of the Elk Hills field since production at the maximum efficient rate was authorized in 1976. The field is located in a non-attainment area for California and National Ambient Air Quality Standards for two criteria pollutants and their associated precursors, is home to four endangered species, and operates within the California regulatory framework. Environmental protection and regulatory compliance is a multi-faceted program carried out through a substantial commitment of resources and workforce involvement. This paper describes the actions taken and resources employed to protect the environment, specific technologies and projects implemented, and the ongoing nature of these efforts at Elk Hills

  13. Meteorological observations in support of a hill cap cloud experiment

    Energy Technology Data Exchange (ETDEWEB)

    Nielsen, Morten

    1998-06-01

    Humid air flows form a hill cap cloud over the Agana mountain ridge in the north-east of Tenerife. The HILLCLOUD project utilised this cloud formation to investigate the chemical and physical properties of cloud aerosols by land based observations. The project was part of the second Aerosol characterisation Experiment (ACE-2) of the International Global Atmospheric chemistry project (IGAC). The present report describes meteorological observations in support of the hill cap cloud experiment. Time-series of wind speed, wind direction, temperature and humidity were collected at ground-based meteorological stations during a period starting one year in advance of the main campaign. A series of radiosonde detecting the upstream stability and wind profile were launched during the main campaign. (au) 5 tabs., 32 ills., 6 refs.

  14. Topographical Hill Shading Map Production Based Tianditu (map World)

    Science.gov (United States)

    Wang, C.; Zha, Z.; Tang, D.; Yang, J.

    2018-04-01

    TIANDITU (Map World) is the public version of National Platform for Common Geospatial Information Service, and the terrain service is an important channel for users on the platform. With the development of TIANDITU, topographical hill shading map production for providing and updating global terrain map on line becomes necessary for the characters of strong intuition, three-dimensional sense and aesthetic effect. As such, the terrain service of TIANDITU focuses on displaying the different scales of topographical data globally. And this paper mainly aims to research the method of topographical hill shading map production globally using DEM (Digital Elevation Model) data between the displaying scales about 1 : 140,000,000 to 1 : 4,000,000, corresponded the display level from 2 to 7 on TIANDITU website.

  15. Generation of terrestrial radiation database in the Larsemann Hills, Antarctica

    International Nuclear Information System (INIS)

    Pal, Rupali; Dhabekar, Bhushan; Jose, Jis Romal; Chinnaesakki, S.; Bakshi, A.K.; Datta, D.; Pradeepkumar, K.S.

    2018-01-01

    Natural background radiation in the environment includes terrestrial radiation, cosmic radiation from space and air activity due to radon/thoron. It is known that cosmic contribution increases near the poles. The terrestrial component is largely due to 232 Th and 238 U series and 40 K. BARC under the cosmic ray dosimetry project with National Centre for Antarctic and Ocean Research (NCAOR) has taken up measurement of natural background radiation at Larsemann Hills, Antarctica. The project includes generation of baseline data on terrestrial radioactivity in water, soil and rock and estimation of cosmic ray doses. Extensive radiation surveys were carried out by the BARC team in the 35 th and 36 th expedition in and around Larsemann hills in East Antarctica where the third Indian station 'Bharati' is situated. This paper presents mapping of terrestrial radiation levels in Antarctica which will help in strengthening the background radiation database and develop a Radiation Informatics System (RIS)

  16. Diversity and biogeography of land snails (Mollusca, Gastropoda) in the limestone hills of Perak, Peninsular Malaysia

    Science.gov (United States)

    Foon, Junn Kitt; Clements, Gopalasamy Reuben; Liew, Thor-Seng

    2017-01-01

    Abstract Limestone hills are now gaining global conservation attention as hotspots for short-range endemic species. Levels of land snail endemism can be high at limestone hills, especially at hill clusters that are geographically isolated. In the State of Perak, Peninsular Malaysia, limestone hills have been opportunistically surveyed for land snails in the past, but the majority have yet to be surveyed. To address this knowledge gap, we systematically surveyed the terrestrial malacofauna of 12 limestone hills that, based on our opinion, are a representation of the limestone land snail assemblages within the State. Our inventory yielded high sampling completeness (>85%). We found 122 species of land snails, of which 34 species were unique to one of the surveyed hills. We identified 30 species that are potentially new to science. The number of land snail species recorded at each hill ranged between 39 and 63 species. Four of the sampled limestone hills namely, Prk 01 G. Tempurung, Prk 55 G. Pondok, Prk 47 Kanthan, and Prk 64 Bt Kepala Gajah, have high levels of species richness and unique species, representing 91% of the total species recorded in this study. We identified two clusters of limestone hills in central Perak with distinct differences in land snail species composition – a northern hill cluster on elevated granite bedrock and southern hill cluster in a low-lying valley surrounded by alluvial soils. As limestone hills continue to be quarried to meet the cement demand, the four identified limestone hills, along with other hills from the two clusters, warrant urgent conservation attention in order to maintain high species diversity within Perak’s terrestrial malacofauna. PMID:28769723

  17. Susceptibility of Shallow Landslide in Fraser Hill Catchment, Pahang Malaysia

    Directory of Open Access Journals (Sweden)

    Wan Nor Azmin Sulaiman

    2010-01-01

    Full Text Available In tropical areas especially during monsoon seasons intense precipitation is the main caused that trigger the natural shallow landslide phenomena. This phenomenon can be disastrous and widespread in occurrence even in undisturbed forested catchment. In this paper, an attempt has been made to evaluate the susceptibility of natural hill slopes to failure for a popular hill resort area, the Fraser Hill Catchment under different rainfall regimes and soil thickness. A Digital Elevation Model (DEM was prepared for the 8.2 km2 catchment. A GIS based deterministic model was then applied to predict the spatial landslide occurrence within catchment. Model input parameters include bulk density, friction angle, cohesion and hydraulic conductivity were gathered through in situ and lab analysis as well as from previous soil analysis records. Landslides locations were recorded using GPS as well as previous air photos and satellite imagery to establish landslide source areas inventory. The landslide susceptibility map was produced under different precipitation event’s simulation to see the effects of precipitation to stability of the hill slopes of the catchment. The results were categorized into naturally unstable (Defended, Upper Threshold, Lower Threshold, marginal instability (Quasi Stable and stable area (Moderately Stable and Stable. Results of the simulation indicated notable change in precipitation effect on Defended area is between 10mm to 40mm range in a single storm event. However, when storm event is exceeded 120mm, the result on Defended area produced by the model tends to be constant further on. For area categorized as naturally unstable (Factor of Safety, SF<1, with 110 mm of precipitation in a single storm event and soil depth at 2 meters and 4 meters could affect 69.51% and 69.88% respectively of the catchment area fall under that class. In addition, the model was able to detect 4% more of the landslide inventory under shallower soil depth of

  18. Do Welsh hill farmers dream of radioactive sheep?

    International Nuclear Information System (INIS)

    Roberts, Gwyn; Williams, Aled; Last, D.

    1993-01-01

    A low-power portable device is being used successfully in North Wales to provide precise position-logging of sheep grazing on upland hill pastures following irradiation by fallout from the Chernobyl reactor. This follows the discovery that radiation levels appear to vary significantly among sheep from the same flock, suggesting hot-spots of radiation. The design and execution of the system is described. (UK)

  19. The Lister Hill National Center for Biomedical Communications.

    Science.gov (United States)

    Smith, K A

    1994-09-01

    On August 3, 1968, the Joint Resolution of the Congress established the program and construction of the Lister Hill National Center for Biomedical Communications. The facility dedicated in 1980 contains the latest in computer and communications technologies. The history, program requirements, construction management, and general planning are discussed including technical issues regarding cabling, systems functions, heating, ventilation, and air conditioning system (HVAC), fire suppression, research and development laboratories, among others.

  20. Mammals of Kalimpong Hills, Darjeeling District, West Bengal, India

    Directory of Open Access Journals (Sweden)

    J.K. Mallick

    2012-10-01

    Full Text Available Neora Valley National Park (NVNP in the Kalimpong Hills, Darjeeling District, having a wide range of altitudinal variations (183-3,200 m and climatic conditions and forming an ecological trijunction with Sikkim and Bhutan, is the last virgin wilderness in West Bengal. It is a global hotspot for the unique ecosystem, where tropical, sub-tropical, temperate and sub-temperate forests represent a wealth of biodiversity including many threatened and rare mammals. It is the prime habitat of Ailurus fulgens (estimated population 28-32, Neofelis nebulosa (population unassessed, Ursus thibetanus (18, Bos gaurus (81, Hemitragus jemlahicus (32, Naemorhedus goral (73, Capricornis sumatraensis (89, Rusa unicolor (286, Muntiacus vaginalis (590 and Sus scrofa (615. Discovery of Panthera tigris (20 in 1998 prompted the forest department to include NVNP as a sensitive wildlife zone. Many authors recorded the mammalian diversity in Darjeeling District since the mid-nineteenth century, but most of them referred to the Darjeeling Hills. The documentations on Kalimpong Hills are scarce because of the dense canopy, thick undergrowth and inaccessible terrain, particularly in the pristine forests of Neora Valley. Consequently, a comprehensive compendium of the mammals in this region was not prepared. A study was undertaken in 2008-2009 with a view to bridging this knowledge-gap and presenting an updated account of the mammalian species in this new short-listed World Heritage Site and surrounding forests of the Kalimpong Hills based on literature review, questionnaire survey, direct sighting and indirect evidences. During June-October 1916, N.A. Baptista recorded 29 mammalian species (22 genera out of 563 specimens collected, from the region. The present study registered 99 species (68 genera after 94 years.

  1. Antimicrobial activity of Davilla elliptica St. Hill (Dilleniaceae

    Directory of Open Access Journals (Sweden)

    D.C. Michelin

    Full Text Available Davilla elliptica St. Hill ("lixinha", family Dilleniaceae, is commonly used in the Brazilian folk medicine as purgative and stimulant. This work evaluated the antimicrobial activity of the methanol and chloroform extracts of the leaves and barks of D. elliptica using the disc-diffusion method. The results obtained showed that the methanolic extracts of the leaves and barks presented antimicrobial activity against the tested microorganisms.

  2. Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology

    Czech Academy of Sciences Publication Activity Database

    Bizzarro, B.; Barros, M.S.; Maciel, C.; Gueroni, D.I.; Lino, C.N.; Campopiano, J.; Kotsyfakis, Michalis; Amarante-Mendes, G.P.; Calvo, E.; Capurro, M.L.; Sa-Nunes, A.

    2013-01-01

    Roč. 6, NOV 2013 (2013), s. 329 ISSN 1756-3305 Institutional support: RVO:60077344 Keywords : dendritic cells * T-cells * Aedes aegypti * saliva * apoptosis Subject RIV: EC - Immunology Impact factor: 3.251, year: 2013

  3. Global dynamics of a PDE model for aedes aegypti mosquitoe incorporating female sexual preference

    KAUST Repository

    Parshad, Rana; Agusto, Folashade B.

    2011-01-01

    In this paper we study the long time dynamics of a reaction diffusion system, describing the spread of Aedes aegypti mosquitoes, which are the primary cause of dengue infection. The system incorporates a control attempt via the sterile insect

  4. Zika virus in Brazil and the danger of infestation by Aedes (Stegomyia mosquitoes

    Directory of Open Access Journals (Sweden)

    Carlos Brisola Marcondes

    2015-01-01

    Full Text Available Abstract Zika virus, already widely distributed in Africa and Asia, was recently reported in two Northeastern Brazilian: State of Bahia and State of Rio Grande do Norte, and one Southeastern: State of São Paulo. This finding adds a potentially noxious virus to a list of several other viruses that are widely transmitted by Aedes (Stegomyia aegypti and Aedes (Stegomyia albopictus in Brazil. The pathology and epidemiology, including the distribution and vectors associated with Zika virus, are reviewed. This review is focused on viruses transmitted by Aedes (Stegomyia mosquitoes, including dengue, Chikungunya, Zika, Mayaro, and yellow fever virus, to emphasize the risks of occurrence for these arboviruses in Brazil and neighboring countries. Other species of Aedes (Stegomyia are discussed, emphasizing their involvement in arbovirus transmission and the possibility of adaptation to environments modified by human activities and introduction in Brazil.

  5. Zika virus in Brazil and the danger of infestation by Aedes (Stegomyia) mosquitoes.

    Science.gov (United States)

    Marcondes, Carlos Brisola; Ximenes, Maria de Fátima Freire de Melo

    2016-02-01

    Zika virus, already widely distributed in Africa and Asia, was recently reported in two Northeastern Brazilian: State of Bahia and State of Rio Grande do Norte, and one Southeastern: State of São Paulo. This finding adds a potentially noxious virus to a list of several other viruses that are widely transmitted by Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus in Brazil. The pathology and epidemiology, including the distribution and vectors associated with Zika virus, are reviewed. This review is focused on viruses transmitted by Aedes (Stegomyia) mosquitoes, including dengue, Chikungunya, Zika, Mayaro, and yellow fever virus, to emphasize the risks of occurrence for these arboviruses in Brazil and neighboring countries. Other species of Aedes (Stegomyia) are discussed, emphasizing their involvement in arbovirus transmission and the possibility of adaptation to environments modified by human activities and introduction in Brazil.

  6. Community-based control of Aedes aegypti by adoption of eco ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    empower women and student communities to estab- lish a sustainable nature ... Control of Aedes aegypti by eco-health methods in Chennai. Pathogens and Global ... predominantly middle class with good/satisfactory housing — often of two ...

  7. DOE to accept bids for Elk Hills crude

    International Nuclear Information System (INIS)

    Anon.

    1992-01-01

    This paper reports that the Department of Energy will accept bids in a reoffering sale covering 53,400 b/d of Elk Hills field oil but later may exercise an option to cut sales volumes and ship 20,000 b/d to Strategic Petroleum Reserve sites in Texas. DOE rejected all 19 bids submitted in an earlier semiannual sale of crude oil from the California naval petroleum reserve, saying they were too low. DOE the, The unique combination of federal and state government policies affecting the movement of oil into and out of the California market has contributed to a situation in which it apparently is very difficult for the government to receive a price for Elk Hills oil that satisfies the minimum price tests that govern the sale of Elk Hills oil. The 12 winning bids in the reoffering sale averaged $13.58/bbl, with bids for the higher quality Stevens zone crude averaging $13.92/bbl, about 67 cents/bbl higher than bids rejected last month. DOE the 20,000 b/d is all local pipelines can ship to the interstate All-American pipeline for transfer to Texas beginning in June

  8. ACCOMMODATION INFRASTRUCTURE AND TOURISM FLOWS ON FELEACU HILL (CLUJ COUNTY

    Directory of Open Access Journals (Sweden)

    DANIELA-LIVIA GHEORGHIEȘ

    2016-06-01

    Full Text Available Accommodation Infrastructure and Tourism Flows on Feleacu Hill (Cluj County. Feleacu Hill experienced tourism development between 2001 and 2015. The INS data indicates that the number of accommodation units increased from one (2001 to four (2015 and there are a few more which are not registered in the INS database. The accommodation capacity increases, as many guesthouses are expanding their premises to receive more tourists and new accommodation units emerge, such as Hotel Premier in Vâlcele (Feleacu commune. Tourism flows also registered a highly positive trend. The number of arrivals increased from 95 tourists in 2002 to 7791 tourists in 2015. However, there was a downturn between 2009 and 2012, due to the economic crisis and the opening of the Turda – Gilău motorway (A3, which redirected transit routes outside the region and led to the closure of Paradis Hotel in 2012. Since 2012, the number of arrivals and overnight stays increased steadily due to the development of new forms of tourism – rural tourism, agrotourism, extreme tourism and complex tourism, materialized in growing numbers of tourists at the two guesthouses in Ciurila commune (“La Mesteceni” and “Domeniul Regilor”. Tourism brings obvious benefits to the rural communities on Feleacu Hill, even if the average duration of stay is still low.

  9. Geologic map of the Bodie Hills, California and Nevada

    Science.gov (United States)

    John, David A.; du Bray, Edward A.; Box, Stephen E.; Vikre, Peter G.; Rytuba, James J.; Fleck, Robert J.; Moring, Barry C.

    2015-01-01

    The Bodie Hills covers about 1,200 km2 straddling the California-Nevada state boundary just north of Mono Lake in the western part of the Basin and Range Province, about 20 km east of the central Sierra Nevada. The area is mostly underlain by the partly overlapping, middle to late Miocene Bodie Hills volcanic field and Pliocene to late Pleistocene Aurora volcanic field (John and others, 2012). Upper Miocene to Pliocene sedimentary deposits, mostly basin-filling sediments, gravel deposits, and fanglomerates, lap onto the west, north, and east sides of the Bodie Hills, where they cover older Miocene volcanic rocks. Quaternary surficial deposits, including extensive colluvial, fluvial, glacial, and lacustrine deposits, locally cover all older rocks. Miocene and younger rocks are tilted ≤30° in variable directions. These rocks are cut by several sets of high-angle faults that exhibit a temporal change from conjugate northeast-striking left-lateral and north-striking right-lateral oblique-slip faults in rocks older than about 9 Ma to north- and northwest-striking dip-slip faults in late Miocene rocks. The youngest faults are north-striking normal and northeast-striking left-lateral oblique-slip faults that cut Pliocene-Pleistocene rocks. Numerous hydrothermal systems were active during Miocene magmatism and formed extensive zones of hydrothermally altered rocks and several large mineral deposits, including gold- and silver-rich veins in the Bodie and Aurora mining districts (Vikre and others, in press).

  10. Age-Stage, Two-Sex Life Table Characteristics of Aedes albopictus and Aedes Aegypti in Penang Island, Malaysia.

    Science.gov (United States)

    Maimusa, Hamisu A; Ahmad, Abu Hassan; Kassim, Nur Faeza A; Rahim, Junaid

    2016-03-01

    The life table developmental attributes of laboratory colonies of wild strains of Aedes albopictus and Aedes aegypti were analyzed and compared based on the age-stage, two-sex life table. Findings inclusive in this study are: adult preoviposition periods, total preoviposition period, mean intrinsic rate of increase (r), mean finite rate of increase (λ), net reproductive rates (R0), and mean generation time (T). The total preadult development time was 9.47 days for Ae. albopictus and 8.76 days for Ae. aegypti. The life expectancy was 19.01 days for Ae. albopictus and 19.94 days for Ae. aegypti. Mortality occurred mostly during the adult stage. The mean development time for each stage insignificantly correlated with temperature for Ae. albopictus (r  =  -0.208, P > 0.05) and (r  =  -0.312, P > 0.05) for Ae. aegypti. The population parameters suggest that Ae. albopictus and Ae. aegypti populations are r-strategists characterized by a high r, a large R0, and short T. This present study provides the first report to compare the life parameters of Ae. albopictus and Ae. aegypti strains from Penang island, Malaysia.

  11. Inferencias sobre Grafos

    OpenAIRE

    Sira M. Allende; Carlos N. Bouza

    2002-01-01

    El estudio de un juego puede ser modelado asumiendo que solo algunas partidas son observadas. Entonces el árbol del juego debe ser estimado utilizando información muestral. Similarmente ocurre al obtener información sobre el comportamiento de las decisiones tomadas por individuos muestreados sobre un árbol de decisión teórico. Al considerar una medida de probabilidad que caracterice el comportamiento de redes aleatorias se puede obtener un estimado del árbol asociado. Este problema es el anal...

  12. Sobre software libre

    OpenAIRE

    Matellán Olivera, Vicente; González Barahona, Jesús; Heras Quirós, Pedro de las; Robles Martínez, Gregorio

    2004-01-01

    220 p. "Sobre software libre" reune casi una treintena de ensayos sobre temas de candente actualidad relacionados con el software libre (del cual Linux es su ex- ponente más conocido). Los ensayos que el lector encontrará están divididos en bloques temáticos que van desde la propiedad intelectual o las cuestiones económicas y sociales de este modelo hasta su uso en la educación y las administraciones publicas, pasando por alguno que repasa la historia del software libre en l...

  13. Biting activity of Aedes scapularis (Rondani and Haemagogus mosquitoes in Southern Brazil (Diptera: Culicidae Atividade hematófaga de mosquitos Aedes scapularis (Rondani e Haemagogus no sul do Brasil (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Oswaldo Paulo Forattini

    1988-04-01

    Full Text Available The biting activity of a population of Aedes scapularis (Rondani, Haemagogus capricornii Lutz and Hg. leucocelaenus (Dyar and Shannon in Southern Brazil was studied between March 1980 and April 1983. Data were obtained with 25-hour human bait catches in three areas with patchy residual forests, named "Jacaré-Pepira", "Lupo" Farm, and "Sta. Helena" Farm, in the highland region of S. Paulo State (Brazil. Data obtained on Ae. scapularis were compared with those formerly gathered in the "Ribeira'' Valley lowlands, and were similar, except in the "Lupo" Farm study area, where a precrepuscular peak was observed, not recorded at the "Jacaré-Pepira" site or in the "Ribeira" Valley. In all the areas this mosquito showed diurnal and nocturnal activity, but was most active during the evening crepuscular period. These observations support the hypothesis about the successful adaptation of Ae. scapularis to man-made environments and have epidemiological implications that arise from it. As for Haemagogus, results obtained on the "Lupo" and "Sta. Helena" regions agree with previous data obtained in several other regions and show its diurnal activity. The proximity of "Lupo" Farm, where Hg. capricornii and Hg. leucocelaenus showed considerable activity, to "Araraquara" city where Aedes aegypti was recently found, raises some epidemiological considerations about the possibility of urban yellow fever resurgence.No período de março de 1980 a abril de 1983 foi estudada a atividade hematófaga de uma população de Aedes scapularis (Rondani, Haemagogus capricornii Lutz, Hg. leucocelaenus (Dyar e Shannon, na região sul do Brasil. Os dados foram obtidos com o emprego de isca humana, mediante captura de 25 horas de duração e correspondente a três áreas, contendo manchas de floresta residual, denominadas Jacaré-Pepira, Fazenda Lupo e Fazenda Santa Helena. Os resultados relativos a Ae. scapularis foram comparados com as coletas anteriormente feitas em áreas de

  14. Oviposition and vertical dispersal of Aedes mosquitoes in multiple storey buildings in Colombo district, Sri Lanka.

    Science.gov (United States)

    Jayathilake, T A Hasini D G; Wickramasinghe, Mervyn B; de Silva, B G D Nissanka K

    2015-09-01

    The Colombo City in Sri Lanka is experiencing tremendous development and construction of multiple storey buildings and high rise apartments. The change in housing types and microhabitats might have altered the flight and breeding behaviour of Aedes mosquito population. This study was carried out to determine the vertical dispersal and abundance of Aedes mosquitoes in multiple storey buildings in the Colombo district, with respect to abiotic factors such as rainfall, humidity and wind speed. Hence, this study is of paramount importance, particularly for planning and implementation of control measures against Aedes mosquitoes. An ovitrap based study was carried out at four selected multiple storey buildings in four residential areas located in Colombo, Sri Lanka, from August to December 2013. Results were analyzed using four indices; ovitrap index, mean number of larvae, mean number of eggs and mean number of larvae per ovipaddle. The results implied that Aedes mosquitoes could be found in different elevations from ground floor to the highest floor (130 ft). There was a significant difference between height and ovitrap index (pAedes mosquitoes (pAedes mosquitoes are able to breed at any level of the buildings and not restricted by their height. The indices (mean number of larvae, mean number of eggs) representing the vertical dispersal with respect to abundance seemed to be statistically non-significant (p>0.05) with height which indicates high abundance of Aedes mosquitoes at higher floors. Abiotic factors also seemed to cause significant effect to the vertical dispersal of Aedes mosquitoes in high rise buildings.

  15. Wolbachia Blocks Currently Circulating Zika Virus Isolates in Brazilian Aedes aegypti Mosquitoes

    OpenAIRE

    Dutra, Heverton Leandro Carneiro; Rocha, Marcele Neves; Dias, Fernando Braga Stehling; Mansur, Simone Brutman; Caragata, Eric Pearce; Moreira, Luciano Andrade

    2016-01-01

    Summary The recent association of Zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the Zika vector, Aedes aegypti mosquitoes. Wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. Here we report that Aedes aegypti harboring Wolbachia are highly resistant to infection with two currently circulating Zika virus isolates from the recent Brazilian epide...

  16. Aedes (Stegomyia) Corneti, A New Species of the Africanus Subgroup (Diptera: Culicidae)

    Science.gov (United States)

    1986-10-14

    virus , chikungunya, Zika and Bouboui viruses have been isolated from Ae. africanus and Ae. opok in the Central African Republic (Germain et al., 1978...J., M. C. Williams, J. P. Woodall, D. I. H. Simpson, and L. K. H. Goma. 1964. Twelve isolations of Zika virus from Aedes (Stegomyiu) africanus...du Banco), Abidjan, Ivory Coast. Medical importance. -Unknown. However, Aedes africanus has been recog- nized as one of the most important virus

  17. Zika virus in Brazil and the danger of infestation by Aedes (Stegomyia) mosquitoes

    OpenAIRE

    Marcondes,Carlos Brisola; Ximenes,Maria de Fátima Freire de Melo

    2016-01-01

    Abstract Zika virus, already widely distributed in Africa and Asia, was recently reported in two Northeastern Brazilian: State of Bahia and State of Rio Grande do Norte, and one Southeastern: State of São Paulo. This finding adds a potentially noxious virus to a list of several other viruses that are widely transmitted by Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus in Brazil. The pathology and epidemiology, including the distribution and vectors associated with Zika virus, are ...

  18. Active metabolites of the genus Piper against Aedes aegypti: natural alternative sources for dengue vector control

    OpenAIRE

    Marques, André M; Kaplan, Maria Auxiliadora C

    2015-01-01

    The mosquito, Aedes aegypti, is the principal vector of the viruses responsible for dengue and dengue hemorrhagic fevers. The mosquito is widespread throughout tropical and sub-tropical regions; its prevalence makes dengue one of the most important mosquito-borne viral diseases in the world occurring annually in more than 100 endemic countries. Because blood is essential to their development cycle, the Aedes species maintains a close association with humans and their dwellings. Fittingly, the...

  19. Evaluation of Three Commercial Backpack Sprayers with Aqualuer (registered trademark) 20-20 Against Caged Adult Aedes aegypti

    Science.gov (United States)

    2016-03-11

    Sprayers with Aqualuer® 20–20 Against Caged Adult Aedes Aegypti Author(s): Derrick Conover, Ali Fulcher, Michael L. Smith, Muhammad Farooq, Marcia K... AEDES AEGYPTI DERRICK CONOVER,1 ALI FULCHER,1 MICHAEL L. SMITH,1 MUHAMMAD FAROOQ,2 MARCIA K. GAINES1 AND RUI-DE XUE1,3 ABSTRACT. Three commercially...adult Aedes aegypti in semifield trials in northeastern Florida. Two battery-powered sprayers, Birchmeier and Hudson, were compared with the standard

  20. The Effects of Pre-Exposure to DEET on the Downstream Blood-Feeding Behaviors of Aedes aegypti Mosquitoes

    Science.gov (United States)

    2016-06-10

    1 Sugiharto et al.: Aedes aegypti blood- Michael J. Turell 1 feeding after DEET pre-exposure Virology Division, USAMRIID 2 1425 Porter...Pre-exposure to DEET on the Downstream Blood-Feeding Behaviors of 9 Aedes aegypti Mosquitoes1 10 11 Victor A. Sugiharto,2 John P. Grieco,2,3...insect behavior for disease prevention. However, genetic insensitivity and 31 habituation in Aedes aegypti (L.) mosquitoes after pre-exposure to DEET

  1. Investigating Potential Effects of Dengue Virus Infection and Pre-exposure to DEET on Aedes aegypti Behaviors

    Science.gov (United States)

    2016-02-05

    Investigating Potential Effects of Dengue Virus Infection and Pre-exposure to DEET on Aedes aegypti Behaviors by Victor A...exposure to DEET on Aedes aegypti Behaviors" Name of Candidate: Victor Sugiharto Doctor of Philosophy Degree February 5, 2016 DISSERTATION AND...Infection and Pre-exposure to DEET on Aedes aegypti Behaviors" is appropriately acknowledged and. beyond brief excerpts , is with the permission of

  2. Identification and Transcription Profiling of NDUFS8 in Aedes taeniorhynchus (Diptera: Culicidae): Developmental Regulation and Environmental Response

    Science.gov (United States)

    2014-12-18

    Identification and transcription profiling of NDUFS8 in Aedes taeniorhynchus (Diptera: Culicidae): developmental regulation and environmental response...7205 Email lmzhao@ufl.edu Abstract: The cDNA of a NADH dehydrogenase-ubiquinone Fe-S protein 8 subunit (NDUFS8) gene from Aedes (Ochlerotatus...information useful for developing dsRNA pesticide for mosquito control. Keywords: Aedes taeniorhynchus, AetNDUFS8, mRNA expression, development

  3. Swarming Mechanisms in the Yellow Fever Mosquito: Aggregation Pheromones are Involved in the Mating Behavior of Aedes aegypti

    Science.gov (United States)

    2014-12-01

    behavior of Aedes aegypti Emadeldin Y. Fawaz1, Sandra A. Allan2, Ulrich R. Bernier2, Peter J. Obenauer3, and Joseph W. Diclaro II1 1Vector Biology... Aedes aegypti swarming behavior and identified associated chemical cues. Novel evidence is provided that Ae. aegypti females aggregate by means of...the isolated aggregation pheromones in controlling Ae. aegypti. Journal of Vector Ecology 39 (2): 347-354. 2014. Keyword Index: Aedes aegypti, swarm

  4. PENGARUH PENGGUNAAN LETHAL OVITRAP TERHADAP POPULASI NYAMUK AEDES SP SEBAGAI VEKTOR DEMAM BERDARAH DENGUE

    Directory of Open Access Journals (Sweden)

    Tri Ramadhani

    2013-09-01

    Full Text Available ABSTRACT. Field evaluation against lethal ovitrap (LO to control dengue vector Aedes mosquitoes carried intwo endemic areas (Bojongsari and Ledug in Banyumas. Lethal ovitrap made ?with the intent to kill mosquito,because mosquitoes will lay eggs containing ovistrip contact with the insecticide and the relatively short time todie.The aim of research to assess the effect of LO applications with the Aedes of mosquito populations sp. Thisstudy includes a quasi experimental design with pretest-posttest control group without randomization. Researchsites in dengue endemic areas with a total sample of 100 houses in each treatment and control areas. Populationsof Aedes sp measured every week for three weeks prior to the intervention and twelve weeks during theintervention. The mean density of Aedes sp compared before and after intervention and between the treatment andcontrol. Results showed mean mosquito density before and after the intervention in the treatment group of 0.07(p-value 0.044, whereas in the control group by 0037 ( p-value 0341. Use LO made ?with the addition ofinsecticide active cypermetrin on ovistrip impact on the density of Aedes sp in the residential neighborhood.Keywords: lethal ovitrap, DHF, Aedes sp

  5. From ground pools to treeholes: convergent evolution of habitat and phenotype in Aedes mosquitoes.

    Science.gov (United States)

    Soghigian, John; Andreadis, Theodore G; Livdahl, Todd P

    2017-12-19

    Invasive mosquito species are responsible for millions of vector-borne disease cases annually. The global invasive success of Aedes mosquitoes such as Aedes aegypti and Aedes albopictus has relied on the human transport of immature stages in container habitats. However, despite the importance of these mosquitoes and this ecological specialization to their widespread dispersal, evolution of habitat specialization in this group has remained largely unstudied. We use comparative methods to evaluate the evolution of habitat specialization and its potential influence on larval morphology, and evaluate whether container dwelling and invasiveness are monophyletic in Aedes. We show that habitat specialization has evolved repeatedly from ancestral ground pool usage to specialization in container habitats. Furthermore, we find that larval morphological scores are significantly associated with larval habitat when accounting for evolutionary relationships. We find that Ornstein-Uhleinbeck models with unique optima for each larval habitat type are preferred over several other models based predominantly on neutral processes, and that OU models can reliably simulate real morphological data. Our results demonstrate that multiple lineages of Aedes have convergently evolved a key trait associated with invasive success: the use of container habitats for immature stages. Moreover, our results demonstrate convergence in morphological characteristics as well, and suggest a role of adaptation to habitat specialization in driving phenotypic diversity in this mosquito lineage. Finally, our results highlight that the genus Aedes is not monophyletic.

  6. Aedes-Borne Virus-Mosquito Interactions: Mass Spectrometry Strategies and Findings.

    Science.gov (United States)

    Pando-Robles, Victoria; Batista, Cesar V

    2017-06-01

    Aedes-borne viruses are responsible for high-impact neglected tropical diseases and unpredictable outbreaks such as the ongoing Zika epidemics. Aedes mosquitoes spread different arboviruses such as Dengue virus (DENV), Chikungunya virus (CHIKV), and Zika virus, among others, and are responsible for the continuous emergence and reemergence of these pathogens. These viruses have complex transmission cycles that include two hosts, namely the Aedes mosquito as a vector and susceptible vertebrate hosts. Human infection with arboviruses causes diseases that range from subclinical or mild to febrile diseases, encephalitis, and hemorrhagic fever. Infected mosquitoes do not show detectable signs of disease, even though the virus maintains a lifelong persistent infection. The infection of the Aedes mosquito by viruses involves a molecular crosstalk between cell and viral proteins. An understanding of how mosquito vectors and viruses interact is of fundamental interest, and it also offers novel perspectives for disease control. In recent years, mass spectrometry (MS)-based strategies in combination with bioinformatics have been successfully applied to identify and quantify global changes in cellular proteins, lipids, peptides, and metabolites in response to viral infection. Although the information about proteomics in the Aedes mosquito is limited, the information that has been reported can set up the basis for future studies. This review reflects how MS-based approaches have extended our understanding of Aedes mosquito biology and the development of DENV and CHIKV infection in the vector. Finally, this review discusses future challenges in the field.

  7. Risk factors for the presence of Aedes aegypti and Aedes albopictus in domestic water-holding containers in areas impacted by the Nam Theun 2 hydroelectric project, Laos.

    Science.gov (United States)

    Hiscox, Alexandra; Kaye, Angela; Vongphayloth, Khamsing; Banks, Ian; Piffer, Michele; Khammanithong, Phasouk; Sananikhom, Pany; Kaul, Surinder; Hill, Nigel; Lindsay, Steven W; Brey, Paul T

    2013-06-01

    We assessed risk factors for vectors of dengue and chikungunya viruses near a new hydroelectric project, Nam Theun 2, in Laos. Immature stages of Aedes aegypti were found only in sites within 40 km of the urban provincial capital, but Aedes albopictus was found throughout. Aedes aegypti pupae were most common in water storage jars (odds ratio [OR] = 4.72) and tires (OR = 2.99), and Ae. albopictus pupae were associated with tires in 2009 (OR = 10.87) and drums, tires, and jars in 2010 (drums OR = 3.05; tires OR = 3.45, jars OR = 6.59). Compared with water storage vessels, containers used for hygiene, cooking, and drinking were 80% less likely to harbor Ae. albopictus pupae in 2010 (OR = 0.20), and discarded waste was associated with a 3.64 increased odds of infestation. Vector control efforts should focus on source reduction of water storage containers, particularly concrete jars and tires.

  8. Genetic Characterization of Spondweni and Zika Viruses and Susceptibility of Geographically Distinct Strains of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus (Diptera: Culicidae) to Spondweni Virus

    Science.gov (United States)

    2016-10-26

    301 policy or position of the U.S. Department of Defense, the Department of the Army, 302 Centers for Disease Control and Prevention, or the Kingdom...Aedes albopicutus (Thailand) 12 0 (0.0) 0 (0.0) Aedes albopicutus ( Venezuela ) 3 0 (0.0) 0 (0.0) Culex quinquefaciatus (Galveston, USA) 24 0 (0.0...albopicutus ( Venezuela ) 24 0 (0.0) 0 (0.0) Culex quinquefaciatus (Galveston, USA) 24 0 (0.0) 0 (0.0) 550 551 552 553 554 555 556 557

  9. Temporal genetic stability of Stegomyia aegypti (= Aedes aegypti) populations.

    Science.gov (United States)

    Gloria-Soria, A; Kellner, D A; Brown, J E; Gonzalez-Acosta, C; Kamgang, B; Lutwama, J; Powell, J R

    2016-06-01

    The mosquito Stegomyia aegypti (= Aedes aegypti) (Diptera: Culicidae) is the primary vector of viruses that cause yellow fever, dengue and Chikungunya fever. In the absence of effective vaccines, the reduction of these diseases relies on vector control strategies. The success of these strategies is tightly linked to the population dynamics of target populations. In the present study, 14 collections from St. aegypti populations separated by periods of 1-13 years were analysed to determine their temporal genetic stability. Although temporal structure is discernible in most populations, the degree of temporal differentiation is dependent on the population and does not obscure the geographic structure of the various populations. The results suggest that performing detailed studies in the years prior to and after population reduction- or modification-based control interventions at each target field site may be useful in assessing the probability of success. © 2016 The Royal Entomological Society.

  10. Targeted genome editing in Aedes aegypti using TALENs.

    Science.gov (United States)

    Aryan, Azadeh; Myles, Kevin M; Adelman, Zach N

    2014-08-15

    The Culicine mosquito, Aedes aegypti, is both a major vector of arthropod-borne viruses (arboviruses) and a genetic model organism for arbovirus transmission. TALE nucleases (TALENs), a group of artificial enzymes capable of generating site-specific DNA lesions, consist of a non-specific FokI endonuclease cleavage domain fused to an engineered DNA binding domain specific to a target site. While TALENs have become an important tool for targeted gene disruption in a variety of organisms, application to the mosquito genome is a new approach. We recently described the use of TALENs to perform heritable genetic disruptions in A. aegypti. Here, we provide detailed methods that will allow other research laboratories to capitalize on the potential of this technology for understanding mosquito gene function. We describe target site selection, transient embryo-based assays to rapidly assess TALEN activity, embryonic microinjection and downstream screening steps to identify target site mutations. Copyright © 2014 Elsevier Inc. All rights reserved.

  11. Modelling the Dynamics of an Aedes albopictus Population

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    Thomas Anung Basuki

    2010-08-01

    Full Text Available We present a methodology for modelling population dynamics with formal means of computer science. This allows unambiguous description of systems and application of analysis tools such as simulators and model checkers. In particular, the dynamics of a population of Aedes albopictus (a species of mosquito and its modelling with the Stochastic Calculus of Looping Sequences (Stochastic CLS are considered. The use of Stochastic CLS to model population dynamics requires an extension which allows environmental events (such as changes in the temperature and rainfalls to be taken into account. A simulator for the constructed model is developed via translation into the specification language Maude, and used to compare the dynamics obtained from the model with real data.

  12. Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection.

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    Michael J Conway

    2016-09-01

    Full Text Available Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV types 1-4. Ae. aegypti transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. Ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. Previously, we showed that Ae. aegypti salivary gland extracts (SGEs enhanced dissemination of DENV to draining lymph nodes. In contrast, HPLC-fractionation revealed that some SGE components inhibited infection. Here, we show that D7 proteins are enriched in HPLC fractions that are inhibitory to DENV infection, and that recombinant D7 protein can inhibit DENV infection in vitro and in vivo. Further, binding assays indicate that D7 protein can directly interact with DENV virions and recombinant DENV envelope protein. These data reveal a novel role for D7 proteins, which inhibits arbovirus transmission to vertebrates through a direct interaction with virions.

  13. Larvicidal activity of some Cerrado plant extracts against Aedes aegypti.

    Science.gov (United States)

    Rodrigues, A M S; De Paula, J E; Degallier, N; Molez, J E; Espindola, L S

    2006-06-01

    One hundred ninety hexanic and ethanolic extract from 27 plant species from the Cerrado biome of Brazil were tested for larvicidal activity against 3rd-stage Aedes aegypti larvae at 500 microg/ml. Fourteen extracts from 7 species showed activity (>65% mortality) against the larvae. Of these Dugeutia furfuracea, Piptocarpha rotundifolia, Casearia sylvestris var. lingua, Serjania lethalis, and Xylopia aromatica were active at 56.6, 162.31, 232.4, 285.76, and 384.37 microg/ml, respectively. Annona crassiflora and Cybistax antisyphilitica showed activity at 23.06 and 27.61 microg/ml. The larvicidal properties of these species are described for the first time, and may prove to be promising in active chemical compound isolation.

  14. The global compendium of Aedes aegypti and Ae. albopictus occurrence

    Science.gov (United States)

    Kraemer, Moritz U. G.; Sinka, Marianne E.; Duda, Kirsten A.; Mylne, Adrian; Shearer, Freya M.; Brady, Oliver J.; Messina, Jane P.; Barker, Christopher M.; Moore, Chester G.; Carvalho, Roberta G.; Coelho, Giovanini E.; van Bortel, Wim; Hendrickx, Guy; Schaffner, Francis; Wint, G. R. William; Elyazar, Iqbal R. F.; Teng, Hwa-Jen; Hay, Simon I.

    2015-07-01

    Aedes aegypti and Ae. albopictus are the main vectors transmitting dengue and chikungunya viruses. Despite being pathogens of global public health importance, knowledge of their vectors’ global distribution remains patchy and sparse. A global geographic database of known occurrences of Ae. aegypti and Ae. albopictus between 1960 and 2014 was compiled. Herein we present the database, which comprises occurrence data linked to point or polygon locations, derived from peer-reviewed literature and unpublished studies including national entomological surveys and expert networks. We describe all data collection processes, as well as geo-positioning methods, database management and quality-control procedures. This is the first comprehensive global database of Ae. aegypti and Ae. albopictus occurrence, consisting of 19,930 and 22,137 geo-positioned occurrence records respectively. Both datasets can be used for a variety of mapping and spatial analyses of the vectors and, by inference, the diseases they transmit.

  15. Invasion Biology of Aedes japonicus japonicus (Diptera: Culicidae)

    Science.gov (United States)

    Fonseca, Dina M.

    2014-01-01

    Aedes japonicus japonicus (Theobald) (Diptera: Culicidae) has recently expanded beyond its native range of Japan and Korea into large parts of North America and Central Europe. Population genetic studies begun immediately after the species was detected in North America revealed genetically distinct introductions that subsequently merged, likely contributing to the successful expansion. Interactions, particularly in the larval stage, with other known disease vectors give this invasive subspecies the potential to influence local disease dynamics. Its successful invasion likely does not involve superior direct competitive abilities, but it is associated with the use of diverse larval habitats and a cold tolerance that allows an expanded seasonal activity range in temperate climates. We predict a continued but slower expansion of Ae. j. japonicus in North America and a continued rapid expansion into other areas as this mosquito will eventually be considered a permanent resident of much of North America, Europe, Asia, and parts of Hawaii. PMID:24397520

  16. Variation in Aedes aegypti Mosquito Competence for Zika Virus Transmission.

    Science.gov (United States)

    Roundy, Christopher M; Azar, Sasha R; Rossi, Shannan L; Huang, Jing H; Leal, Grace; Yun, Ruimei; Fernandez-Salas, Ildefonso; Vitek, Christopher J; Paploski, Igor A D; Kitron, Uriel; Ribeiro, Guilherme S; Hanley, Kathryn A; Weaver, Scott C; Vasilakis, Nikos

    2017-04-01

    To test whether Zika virus has adapted for more efficient transmission by Aedes aegypti mosquitoes, leading to recent urban outbreaks, we fed mosquitoes from Brazil, the Dominican Republic, and the United States artificial blood meals containing 1 of 3 Zika virus strains (Senegal, Cambodia, Mexico) and monitored infection, dissemination, and virus in saliva. Contrary to our hypothesis, Cambodia and Mexica strains were less infectious than the Senegal strain. Only mosquitoes from the Dominican Republic transmitted the Cambodia and Mexica strains. However, blood meals from viremic mice were more infectious than artificial blood meals of comparable doses; the Cambodia strain was not transmitted by mosquitoes from Brazil after artificial blood meals, whereas 61% transmission occurred after a murine blood meal (saliva titers up to 4 log 10 infectious units/collection). Although regional origins of vector populations and virus strain influence transmission efficiency, Ae. aegypti mosquitoes appear to be competent vectors of Zika virus in several regions of the Americas.

  17. Developmental neurogenetics of sexual dimorphism in Aedes aegypti.

    Science.gov (United States)

    Duman-Scheel, Molly; Syed, Zainulabeuddin

    Sexual dimorphism, a poorly understood but crucial aspect of vector mosquito biology, encompasses sex-specific physical, physiological, and behavioral traits related to mosquito reproduction. The study of mosquito sexual dimorphism has largely focused on analysis of the differences between adult female and male mosquitoes, particularly with respect to sex-specific behaviors related to disease transmission. However, sexually dimorphic behaviors are the products of differential gene expression that initiates during development and therefore must also be studied during development. Recent technical advancements are facilitating functional genetic studies in the dengue vector Aedes aegypti, an emerging model for mosquito development. These methodologies, many of which could be extended to other non-model insect species, are facilitating analysis of the development of sexual dimorphism in neural tissues, particularly the olfactory system. These studies are providing insight into the neurodevelopmental genetic basis for sexual dimorphism in vector mosquitoes.

  18. Seasonal monitoring of Aedes albopictus: practical applications and outcomes

    Directory of Open Access Journals (Sweden)

    Stefano Gavaudan

    2014-06-01

    Full Text Available The introduction of the Asian tiger mosquito Aedes (Stegomya albopictus (Diptera: Culicidae into temperate regions poses serious concerns for the risk of the spreading of arboviral epidemics, as confirmed by the Chikungunya fever outbreak in Italy. This article describes the implementation and the results of a strategy for the pest management implemented over 4 years in Pesaro (a city in the Marche region, Italy. The strategy used 60 integrated wide‑sized ovitraps for monitoring purposes. Twenty-day larvicide-based treatment cycles were implemented for the manholes of the urban area and also the inhabitants were involved in pest control relating to their own properties. It was observed that the weekly median of eggs laid decreased consistently from 2008 to 2011, indicating the good performance of the vector control and a reduction in the related epidemics risk.

  19. Developmental neurogenetics of sexual dimorphism in Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Molly eDuman-Scheel

    2015-06-01

    Full Text Available Sexual dimorphism, a poorly understood but crucial aspect of vector mosquito biology, encompasses sex-specific physical, physiological, and behavioral traits related to mosquito reproduction. The study of mosquito sexual dimorphism has largely focused on analysis of the differences between adult female and male mosquitoes, particularly with respect to sex-specific behaviors related to disease transmission. However, sexually dimorphic behaviors are the products of differential gene expression that initiates during development and therefore must also be studied during development. Recent technical advancements are facilitating functional genetic studies in the dengue vector Aedes aegypti, an emerging model for mosquito development. These methodologies, many of which could be extended to other non-model insect species, are facilitating analysis of the development of sexual dimorphism in neural tissues, particularly the olfactory system. These studies are providing insight into the neurodevelopmental genetic basis for sexual dimorphism in vector mosquitoes.

  20. Toksisitas Insektisida Organofosfat Dan Karbamat Terhadap Nyamuk Aedes aegypti

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    Endang Puji Astuti

    2013-02-01

    Full Text Available Abstract. Aedes aegypti mosquito is increasing problem of public health, being the vector responsible for Dengue and Chikungunya. Chlorpirifos (Organofosfat and Metonil (Carbamate were known to posses insecticide activity against insect. The study was aimed to examine effectiveness of Chlorpirifos and Metonil as insectiside against Ae. aegypti mos­quito Chlorpirifos a significantly higher insecticide activity against Ae. aegypti_than Metonil. The mosquito mortality was observed after 24 h exposure. The LCso value of Chlor­pirifos and Metonil were 0.64 mg/lt and 0,802 mg/lt, against Ae. aegypti mosquito. The mixed of both insecticide was LCso value 108.04 mg/lt, this result prove that mixed of both insecticede not sinergism. The result of th is study suggested that Chlorpirifos more effective insecticide against Ae. aegypti than Metonil. Key Words : Culex quinquefasciatus, insecticide, chlorpirifos, metonil

  1. Repellents inhibit P450 enzymes in Stegomyia (Aedes aegypti.

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    Gloria Isabel Jaramillo Ramirez

    Full Text Available The primary defence against mosquitoes and other disease vectors is often the application of a repellent. Despite their common use, the mechanism(s underlying the activity of repellents is not fully understood, with even the mode of action of DEET having been reported to be via different mechanisms; e.g. interference with olfactory receptor neurones or actively detected by olfactory receptor neurones on the antennae or maxillary palps. In this study, we discuss a novel mechanism for repellence, one of P450 inhibition. Thirteen essential oil extracts from Colombian plants were assayed for potency as P450 inhibitors, using a kinetic fluorometric assay, and for repellency using a modified World Health Organisation Pesticide Evaluations Scheme (WHOPES arm-in cage assay with Stegomyia (Aedes aegypti mosquitoes. Bootstrap analysis on the inhibition analysis revealed a significant correlation between P450-inhibition and repellent activity of the oils.

  2. Coexistence of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Peninsular Florida Two Decades After Competitive Displacements.

    Science.gov (United States)

    Lounibos, L Philip; Bargielowski, Irka; Carrasquilla, María Cristina; Nishimura, Naoya

    2016-11-01

    The spread of Aedes albopictus (Skuse) eastward in the mid-1980s from its initial establishment in Houston, TX, was associated with rapid declines and local disappearances of Aedes aegypti (L.) in Gulf Coast states and Florida where annual larval surveillance during the early 1990s described temporal and spatial patterns of competitive displacements in cemeteries and tire shops. Approximately 20 yr later in 2013-2014, we re-visited former collection sites and sampled aquatic immatures of these two species from tire shops in 10 cities on State Route 441 and from 9 cemeteries from Lakeland to Miami in southwest Florida. In the recent samples Ae. aegypti was recovered from three central Florida cities where it had not been detected in 1994, but its northern limit on Rte. 441, Apopka, did not change. Other evidence, such as trends at a few cemeteries, suggested a moderate resurgence of this species since 1994. Cage experiments that exposed female progeny of Ae. aegypti from recent Florida collection sites to interspecific mating by Ae. albopictus males showed that females from coexistence sites had evolved resistance to cross-mating, but Ae. aegypti from sites with no Ae. albopictus were relatively susceptible to satyrization. Habitat classifications of collection sites were reduced by principal component (PC) analysis to four variables that accounted for > 99% of variances; PCs with strong positive loadings for tree cover and ground vegetation were associated with collection sites yielding only Ae. albopictus Within the coexistence range of the two species, the numbers of Ae. aegypti among total Aedes collected were strongly correlated in stepwise logistic regression models with two habitat-derived PCs, distance from the coast, and annual rainfall and mean maximum temperatures at the nearest weather station. Subtle increases in the range of Ae. aegypti since its previous displacements are interpreted in the context of the evolution of resistance to mating

  3. Biologi dan Peranan Aedes albopictus (Skuse 1894 sebagai Penular Penyakit

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    Hasan Boesri

    2013-02-01

    Full Text Available Abstracts. Behavior of the mosquito Aedes albopictus is generally res ting outside the home with the brood in a natural or artificial containers protected from sunlight. Human biting activity between the hours of9:00 to 11:00 and between the hours of 17:00 to 18:00 inside and outside the home. The period of rest after sucking the blood 4-5 days and is ready to lie. Habitat or the environment that most coveted of th is mosquito is a forest or gar­den with temperatures of 24-30 0 C. eggs hatch after 4-5 days with a temperature of 24-30 0 C, the eggs usually form elus ters of 49-60 eggs Larvae and pupae usually found in contain­ers, pieces of bambo containing water. The period of the larvae to adults between 20-25 days. The spread of Ae albopictus mosquitoes from Africa, India, Pakistan, Sri Lanka, Thai­land, Malaysia, Vietnam, Papua New Guinea, northern Australia, and Indonesia. Role in disease transmission is a secondary vector or as the primary vector of dengue hemorrhagic fever. On viral diseases that attack the nerves like encephalistis Japanese, Western or East­ern encephalistis, and Chikuguya has been demonstrated by laboratories, as well as on ani­mal diseases caused by Dirofilaria immitis agent, Plasmodium lophurae, P. gallinaceum, and P. fallax.Keyword : Aedes albopictus mosquito, bionomic, the habit of biting / looking for blood, the ability to fly

  4. Aedes albopictus and Its Environmental Limits in Europe.

    Science.gov (United States)

    Cunze, Sarah; Kochmann, Judith; Koch, Lisa K; Klimpel, Sven

    2016-01-01

    The Asian tiger mosquito Aedes albopictus, native to South East Asia, is listed as one of the worst invasive vector species worldwide. In Europe the species is currently restricted to Southern Europe, but due to the ongoing climate change, Ae. albopictus is expected to expand its potential range further northwards. In addition to modelling the habitat suitability for Ae. albopictus under current and future climatic conditions in Europe by means of the maximum entropy approach, we here focused on the drivers of the habitat suitability prediction. We explored the most limiting factors for Aedes albopictus in Europe under current and future climatic conditions, a method which has been neglected in species distribution modelling so far. Ae. albopictus is one of the best-studied mosquito species, which allowed us to evaluate the applied Maxent approach for most limiting factor mapping. We identified three key limiting factors for Ae. albopictus in Europe under current climatic conditions: winter temperature in Eastern Europe, summer temperature in Southern Europe. Model findings were in good accordance with commonly known establishment thresholds in Europe based on climate chamber experiments and derived from the geographical distribution of the species. Under future climatic conditions low winter temperature were modelled to remain the most limiting factor in Eastern Europe, whereas in Central Europe annual mean temperature and summer temperatures were modelled to be replaced by summer precipitation, respectively, as most limiting factors. Changes in the climatic conditions in terms of the identified key limiting factors will be of great relevance regarding the invasive potential of the Ae. albopictus. Thus, our results may help to understand the key drivers of the suggested range expansion under climate change and may help to improve monitoring programmes. The applied approach of investigating limiting factors has proven to yield valuable results and may also provide

  5. Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.

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    Davi F. Farias

    2010-09-01

    Full Text Available This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100% of mortalit y after 1 to 3 h of exposure. They showed LC50 and LC90 values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high soluble proteins content (0.98 to 7.71 mg/mL, lectin (32 to 256 HU/mL and trypsin inhibitory activity (3.64 = 0.43 to 26.19 = 0.05 gIT/kg of flour The electrophoretic profiles showed a great diversity of protein bands, many of which already described as insecticide proteins. The extracts showed low toxicity to mice (LD50 > 0.15 = 0.01 g/kg body weight, but despite these promising results, further studies are necessary to understand the toxicity of these extracts and their constituentsfrom primary and secondary metabolism upon Ae. aegypti.Este trabalho objetivou avaliar a toxicidade dos extratos aquosos de sementes de 15 espécies de leguminosas contra larvas de Aedes aegypti. Foi realizada uma caracterização química e bioquímica parcial dos extratos aquosos e a avaliação da toxicidade aguda em camundongos. Os extratos de Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum e Piptadenia moniliformis causaram 100% de mortalidade depois de 1 a 3 h de exposição e mostraram valores de CL50 e CL90 entre 0,43 = 0,01 e 9,06 ± 0,12 e entre 0,71 = 0,02 e 13,03 = 0,15 mg/mL, respectivamente. Dentre os constituintes do metabolismo secundário, os extratos

  6. Jumlah Telur Aedes Spp yang Terperangkap di Kelurahan Jati Kota Padang

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    Gusti Rati

    2016-08-01

    Full Text Available AbstrakAedes spp adalah vektor pembawa virus dengue yang dapat menimbulkan penyakit Demam Berdarah Dengue (DBD. DBD merupakan penyakit menular yang cenderung mengalami peningkatan dan penyebarannya semakin luas. Cara mengurangi penyebaran penyakit DBD dengan mengendalikan vektor, salah satunya dengan memutus siklus hidup vektor dengan  menggunakan perangkap telur/pemasangan ovitrap. Tujuan penelitian ini adalah untuk membandingkan efektivitas berbagai media ovitrap terhadap jumlah telur Aedes spp yang terperangkap. Jenis penelitian ini adalah eksperimental quasi dengan rancangan post test only design. Populasi dalam penelitian ini adalah semua telur Aedes spp yang terperangkap pada ovitrap, sampel diambil dari sepuluh rumah dengan ulangan enam kali. Lokasi penelitian adalah RW I, III, V, VII, IX Kelurahan Jati. Variabel bebas adalah berbagai media (air sumur, air jerami, air kolam, air mineral ovitrap. Variabel terikat adalah jumlah telur Aedes spp yang terperangkap. Uji normalitas data menggunakan uji kolmogorov smirnov, data terdistribusi normal (p>0,05 dan uji varian data tidak homogen (p<0,05, maka dgunakan uji Kruskal Walls dan Mann Withney. Hasil penelitian diperoleh telur nyamuk Aedes spp yang terperangkap selama penelitian adalah 3.090 butir dengan sebaran 1.563 butir di luar rumah dan 1.527 butir di dalam rumah. Berdasarkan media ovitrap, telur yg terperangkap pada media air jerami 1.758 butir, air mineral 576 butir, air kolam 523 butir, air sumur 233 butir. Uji statistik mendapatkan p = 0,000. Hal ini menunjukkan bahwa terdapat perbedaan jumlah telur Aedes spp yang terdapat pada masing-masing media ovitrap.  Disimpulkan bahwa air jerami lebih efektif sebagai media ovitrap daripada  air mineral air kolam, air sumur.    Kata kunci: telur Aedes spp, ovitrap, media ovitrap AbstractAedes spp are vectors of dengue viruses that can cause Dengue Hemorrhagic Fever (DHF. Dengue is one of the infectious diseases that the number of

  7. The proterozoic Georgetown Province - a Broken Hill analogue

    International Nuclear Information System (INIS)

    Carr, G.R.

    2000-01-01

    Collaborative work between CSIRO and AGSO has resulted in the development of a Pb isotope model that attempts to place relatively precise (∼5 Ma) age constraints on Proterozoic mineralisation in the Mount Isa and McArthur River terrains (Sun et al., 1994). Although this model was developed for sediment hosted mineralisation in low grade metamorphic terrains, the CSIRO-AGSO model ages for other mineralisation in high-grade terrains such as Broken Hill appear to be consistent with the U-Pb zircon ages obtained for the high-grade host sequences. Without independent evidence that the model is applicable to such terrains, the observations cannot be used to indicate the age of the mineralisation. Lead isotope data obtained on potassium feldspar separates from five felsic intrusive samples in the Georgetown terrain show a wide range of Pb isotope ratios. The lowest 206 Pb/ 204 Pb analyses are considered to approximate to the Proterozoic initial ratio and indicate a model age of ∼1510 Ma based on the CSIRO-AGSO model. This age is 45 Ma younger than the crystallisation age of the granite, but must be considered a minimum as the initial 206 Pb/ 204 Pb ratio may well prove to be lower after more comprehensive analysis. Sulfide mineralisation within the Einasleigh Metamorphics has a wide range of 206 Pb/ 204 Pb ratios that lie between this granite value and the relatively homogeneous population from Railway Flat. The Railway Flat data are very similar to values for Broken Hill and also the Broken Hill-type Pegmont mineralisation in the Mount Isa Eastern Succession. These data all have significantly lower 207 Pb/ 204 Pb ratios than the CSIRO-AGSO model, suggesting a significantly different source rock environment for this style of mineralisation from that for the sediment hosted deposits

  8. Survei Larva Nyamuk Aedes Vektor Demam Berdarah Dengue di Kelurahan Kuranji Kecamatan Kuranji Kotamadya Padang Provinsi Sumatera Barat

    Directory of Open Access Journals (Sweden)

    Muhammad Arifudin

    2016-01-01

    Full Text Available AbstrakDemam berdarah dengue merupakan salah satu masalah kesehatan masyarakat yang masih ditemukan di Indonesia. Penyakit ini disebabkan oleh virus Dengue yang ditularkan melalui gigitan nyamuk Aedes. Kelurahan Kuranji merupakan kelurahan dengan kasus demam berdarah dengue terbanyak di Kota Padang. Tujuan penelitian ini adalah menentukan density figure (kepadatan larva nyamuk Aedes di Kelurahan Kuranji Padang. Penelitian dilaksanakan di Kelurahan Kuranji pada bulan April 2014. Desain penelitian adalah survei deskriptif dengan jumlah sampel 50 buah rumah yang diambil dengan cara multistage sampling. Hasil penelitian menunjukkan bahwa aedes aegypti ditemukan lebih banyak dibandingkan aedes albopictus, yaitu  97,74%. house index yaitu 52,50%, container index yaitu 34,72%,  breteau index yaitu 66,50% dan density figure yaitu 7.  Hasil penelitian ini menunjukkan bahwa aedes aegypti lebih banyak ditemukan di dalam rumah dan aedes albopictus hanya ditemukan di luar rumah. Bak mandi dari semen merupakan kontainer yang paling banyak ditemukan larva Aedes dibandingkan jenis kontainer lain.Kata kunci: dengue, aedes, density figure AbstractDengue haemorraghic fever is one of  important public health problem in Indonesia. This disease is caused by the dengue virus which is transmitted by  Aedes bytes. Kuranji is the most prevalent dengue haemorraghic fever in Padang.The objective of this study was to determine density figure in Kuranji district. This research had been held in Kuranji district in April 2014. The design was descriptive survey research method. The number of sampel was 50 that was taken by multi stage sampling method. The result showed that aedes aegypti found more than aedes albopictus, about 97.74%. house index 52.50%, container index 34.72%,  breteau index 66.50% and density figure 7. The research showed that Aedes aegypti is mostly found at house and aedes albopictus only found outdoor. The bath tank which are made from cement is the

  9. Molecular and Phytochemical Investigation of Angelica dahurica and Angelica pubescentis Essential Oils and Their Biological Activity against Aedes aegypti, Stephanitis pyrioides, and Colletotrichum Species

    Science.gov (United States)

    2014-08-18

    Molecular and Phytochemical Investigation of Angelica dahurica and Angelica pubescentis Essential Oils and Their Biological Activity against Aedes ...against yellow fever mosquito, Aedes aegypti, and azalea lace bugs, Stephanitis pyrioides, than A. pubescentis root oil. The major compounds in the A...dahurica, Angelica pubescentis, internal transcribed spacer region, Colletotrichum species, Aedes aegypti, Stephanitis pyrioides, 1-dodecanol, 1

  10. Taming a Tiger in the City: Comparison of Motorized Backpack Applications and Source Reduction Against the Asian Tiger Mosquito, Aedes albopictus

    Science.gov (United States)

    2014-06-01

    TAMING A TIGER IN THE CITY: COMPARISON OF MOTORIZED BACKPACK APPLICATIONS AND SOURCE REDUCTION AGAINST THE ASIAN TIGER MOSQUITO, AEDES ALBOPICTUS...FONSECA2 AND ARY FARAJOLLAHI2,3,7 ABSTRACT. We evaluated 2 strategies to manage Aedes albopictus: 1) motorized backpack applications and 2) source...the community level. KEY WORDS Bacillus thuringiensis israelensis, Bti, methoprene, hand applications, backpack applications INTRODUCTION Aedes

  11. Water quality in vicinity of Fenton Hill Site, 1974

    International Nuclear Information System (INIS)

    Purtymun, W.D.; Adams, W.H.; Owens, J.W.

    1975-09-01

    The water quality at nine surface water stations, eight ground water stations, and the drilling operations at the Fenton Hill Site have been studied as a measure of the environmental impact of the Los Alamos Scientific Laboratory geothermal experimental studies in the Jemez Mountains. Surface water quality in the Jemez River drainage area is affected by the quality of the inflow from thermal and mineral springs. Ground water discharges from the Cenozoic Volcanics are similar in chemical quality. Water in the main zone of saturation penetrated by test hole GT-2 is highly mineralized, whereas water in the lower section of the hole, which is in granite, contains a higher concentration of uranium

  12. La Ecuación de Hill con Potencial Irregular

    Directory of Open Access Journals (Sweden)

    Santiago Cambronero

    2012-03-01

    Full Text Available Se considera la ecuación de Hill cuyo potencial es la derivada formal de una función Hölder - continua de parámetro \\theta \\in (0,1 y se muestra que las soluciones de la versión discreta correspondiente convergen adecuadamente a las soluciones de la ecuación original. Este hecho se usa para establecer teoremas de existencia de soluciones para este caso singular y para deducir algunas propiedades de las soluciones y el discriminante de la ecuación estudiada.

  13. The Hill-determinant perturbation theory with triangular propagators

    International Nuclear Information System (INIS)

    Znojil, M.

    1996-01-01

    A new version of the Rayleigh-Schroedinger perturbation prescription is proposed. Its main formal feature lies in an unusual choice of the model space and unperturbed H 0 and in a resulting lower-triangular matrix structure of its propagators. Within the framework of the so-called Hill-determinant method, an admissibility of any incompletely solvable zero-order Hamiltonian is achieved in this way. As a consequence, the range of practical applicability of our new perturbative formalism may be expected to incorporate many new phenomenological interactions with a strongly anharmonic character. 18 refs

  14. Iowa Hill Pumped Storage Project Investigations - Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Hanson, David [Sacramento Municipal Unitlity District, Sacramento, CA (United States)

    2016-07-01

    This Final Technical Report is a summary of the activities and outcome of the Department of Energy (DOE) Assistance Agreement DE-EE0005414 with the Sacramento Municipal Utility District (SMUD). The Assistance Agreement was created in 2012 to support investigations into the Iowa Hill Pumped-storage Project (Project), a new development that would add an additional 400 MW of capacity to SMUD’s existing 688MW Upper American River Hydroelectric Project (UARP) in the Sierra Nevada mountains east of Sacramento, California.

  15. Inferencias sobre Grafos

    Directory of Open Access Journals (Sweden)

    Sira M. Allende

    2002-05-01

    Full Text Available El estudio de un juego puede ser modelado asumiendo que solo algunas partidas son observadas. Entonces el árbol del juego debe ser estimado utilizando información muestral. Similarmente ocurre al obtener información sobre el comportamiento de las decisiones tomadas por individuos muestreados sobre un árbol de decisión teórico. Al considerar una medida de probabilidad que caracterice el comportamiento de redes aleatorias se puede obtener un estimado del árbol asociado. Este problema es el analizado en este trabajo. Algunos resultados experimentales obtenidos usando Recocido Simulado ilustran el procedimiento. Su papel en el estudio de modelos económicos y de mercadeo es discutido.

  16. Escrito sobre el cuerpo

    Directory of Open Access Journals (Sweden)

    Antonio Malalana Ureña

    2013-09-01

    Full Text Available El catálogo, en una edición bilingüe español-inglés, es una pequeña guía de la exposición Shirin Neshat. Escrito sobre el cuerpo, muestra que se integra dentro de los actos de PHOTOESPAÑA13, el festival internacional de fotografía y artes visuales. El esquema del libro es sencillo y los textos son extremadamente divulgativos. El primero de ellos, Escrito sobre el cuerpo (pp. 10-15, cuyo autor es Octavio Zaya, comisario de la exposición, resume la trayectoria de esta intelectual, que "interactúa" con la fotografía y los formatos audiovisuales, y nos presenta la argumentación narrativa de los documentos expuestos, contextualizando la obra recopilada.

  17. Updated Reported Distribution of Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus (Diptera: Culicidae) in the United States, 1995-2016.

    Science.gov (United States)

    Hahn, Micah B; Eisen, Lars; McAllister, Janet; Savage, Harry M; Mutebi, John-Paul; Eisen, Rebecca J

    2017-09-01

    Aedes (Stegomyia) aegypti (L.) and Aedes (Stegomyia) albopictus (Skuse) are potential vectors of Zika, dengue, and chikungunya viruses in the United States. A Zika virus outbreak in Florida in the summer of 2016, driven by Ae. aegypti and resulting in > 200 locally acquired cases of human illness, underscored the need for up-to-date information on the geographic distribution of Ae. aegypti and Ae. albopictus in the United States. In early 2016, we conducted a survey and literature review to compile county records for presence of Ae. aegypti and Ae. albopictus in the United States from 1995 to 2016. Surveillance for these vectors was intensified across the United States during the summer and fall of 2016. At the end of 2016, we therefore conducted a follow-up survey of mosquito control agencies, university researchers, and state and local health departments to document new collection records for Ae. aegypti and Ae. albopictus. The repeated survey at the end of the year added Ae. aegypti collection records from 38 new counties and Ae. albopictus collection records from 127 new counties, representing a 21 and 10 percent increase, respectively, in the number of counties with reported presence of these mosquitoes compared with the previous report. Moreover, through our updated survey, 40 and 183 counties, respectively, added additional years of collection records for Ae. aegypti and Ae. albopictus from 1995 to 2016. Our findings underscore the continued need for systematic surveillance of Ae. aegypti and Ae. albopictus. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.

  18. Screening for larvicidal activity of ethanolic and aqueous extracts of selected plants against Aedes aegypti and Aedes albopictus larvae

    Directory of Open Access Journals (Sweden)

    Michael Russelle Alvarez

    2016-02-01

    Full Text Available Objective: To screen for larvicidal activity of aqueous and ethanolic extracts (95% ethanol from Selaginella elmeri, Christella dentata, Elatostema sinnatum, Curculigo capitulata, Euphorbia hirta, Murraya koenigii (M. koenigii, Alpinia speciosa, Cymbopogon citratus, Eucalyptus globulus (E. globulus, Jatropha curcas (J. curcas, Psidium guajava, Gliricidia sepium, Ixora coccinea and Capsicum frutescens (C. frutescens against Aedes aegypti (A. aegypti and Aedes albopictus (A. albopictus 3rd instar larvae. Methods: Ethanolic and aqueous extracts were screened for larvicidal activity by exposing the A. aegypti and A. albopictus 3rd instar larvae (15 larvae per trial, triplicates for 48 h, counting the mortalities every 24 h. Additionally, phytochemical screening for flavonoids, tannins, alkaloids, anthraquinones, anthrones, coumarins, indoles and steroids were performed on active extracts using spray tests. Results: Against A. aegypti, the three most active extracts were C. frutescens ethanolic (100% after 24 and 48 h, J. curcas ethanolic (84.44% after 24 h and 88.89% after 48 h and M. koenigii ethanolic (53.33% after 24 h and 71.11% after 48 h. On the other hand, against A. albopictus, the three most active extracts were C. frutescens ethanolic (93.33% after 24 h and 100% after 48 h, J. curcas ethanolic (77.78% after 24 h and 82.22% after 48 h and E. globulus ethanolic (64.44% after 24 h and 73.33% after 48 h. Phytochemical screening was also performed on the active extracts, revealing alkaloids, tannins, indoles and steroids. Conclusios: The results demonstrate the larvicidal activities of ethanolic extracts of Cymbopogon citratus, Euphorbia hirta, Ixora coccinea, Gliricidia sepium, M. koenigii, E. globulus, J. curcas and C. frutescens against A. aegypti and A. albopictus 3rd instar larvae. These could be used as potential larvicidal agents for the control of these mosquitoes.

  19. Audio-magnetotelluric (AMT) study to investigate the genesis of Mujil hill

    Science.gov (United States)

    Rahmania, Suryanto, Wiwit

    2017-07-01

    Gunung Mujil is an isolated hill located near Pondoworejo village, Kalibawang sub-district, Kulon Progo district, and Special Province of Yogyakarta. The hill is part of the eastern Kulon Progo mountain range extended relatively in the North-South direction. The lithology of the hill consists of andesite breccia and it's similar with the Old Andesite Formation that built the Kulon Progo Mountains. There are at least two hypothesis about the genesis and the formation mechanism of this hill, (1) it was formed by debris mass from Kulon Progo Mountains, and (2) ) it was formed by an intrusion. Our study intended to determine the subsurface resistivity below the hill and to relating those results to with the scenario of the genesis of the Mujil hill. We conducted Audio-magnetotellurics (AMT) measurements along two lines survey crossing the Mujil hill consisting of 20 measurements. Since the measurements are located near the villages, most of the data has a fair to bad quality and only one station yielded an excellent data. A 1D Forward modeling was then applied to find best-fit model of the AMT data. The results shows that the Mujil hill was built by debris mass of the Old Andesite Formation from Kulon Progo mountain which is represented by a lower resistivity value under the Mujil hill.

  20. Wine Industry Competitiveness: A survey of the Shawnee Hills American Viticultural Area

    Directory of Open Access Journals (Sweden)

    C. Matthew Rendleman

    2016-06-01

    Shawnee Hill׳s AVA winery owner/operators regard increases in regional tourism, growth in the US wine market continuous innovation, unique services and processes, and flow of information from customers to have the most enhancing effects on their businesses, and that confidence/trust in Illinois state political systems, tax systems, and administrative/bureaucratic regulations were the most constraining factors. Furthermore the Shawnee Hills AVA has growing competition, yet consists of innovative winery owners. It may currently lack external financial support, but with a community focus on product differentiation, the Shawnee Hills AVA has a chance, owners believe, to capture a portion of the growing market for regional products.

  1. Soils of wet valleys in the Larsemann Hills and Vestfold Hills oases (Princess Elizabeth Land, East Antarctica)

    Science.gov (United States)

    Mergelov, N. S.

    2014-09-01

    The properties and spatial distribution of soils and soil-like bodies in valleys of the coastal Larsemann Hills and Vestfold Hills oases—poorly investigated in terms of the soil areas of East Antarctica—are discussed. In contrast to Dry Valleys—large continental oases of Western Antarctica—the studied territory is characterized by the presence of temporarily waterlogged sites in the valleys. It is argued that the deficit of water rather than the low temperature is the major limiting factor for the development of living organisms and the pedogenesis on loose substrates. The moisture gradients in the surface soil horizons explain the spatial distribution of the different soils and biotic complexes within the studied valleys. Despite the permanent water-logging of the deep suprapermafrost horizons of most of the soils in the valleys, no gley features have been identified in them. The soils of the wet valleys in the Larsemann Hills oasis do not contain carbonates. They have a slightly acid or neutral reaction. The organic carbon and nitrogen contents are mainly controlled by the amount of living and dead biomass rather than by the humic substances proper. The larger part of the biomass is concentrated inside the mineral soil matrix rather than on the soil surface. The stresses caused by surface drying, strong winds, and ultraviolet radiation prevent the development of organisms on the surface of the soil and necessitate the search for shelter within the soil fine earth material (endoedaphic niche) or under the gravelly pavement (hypolithic niche). In the absence of higher plants, humified products of their decomposition, and rainwater that can wash the soil profile and upon the low content of silt and clay particles in the soil material, "classical" soil horizons are not developed. The most distinct (and, often, the only diagnosed) products of pedogenesis in these soils are represented by organomineral films on the surface of mineral particles.

  2. The effect of Piper aduncum Linn. (Family: Piperaceae) essential oil as aerosol spray against Aedes aegypti (L.) and Aedes albopictus Skuse.

    Science.gov (United States)

    Misni, Norashiqin; Othman, Hidayatulfathi; Sulaiman, Sallehudin

    2011-08-01

    The bioefficacy of Piper aduncum L. essential oil formulated in aerosol cans was evaluated against Aedes aegypti and Aedes albopictus in a simulated room. The aerosol spray test was based on the Malaysian test standard for aerosol (MS 1221:1991UDC 632.982.2 modified from WHO 2009 methodology) and examined the knockdown effect within 20 minutes of exposure. Mortality rate after 24 hour of holding period was also determined. A commercial aerosol spray (0.09% prallethrin 0.05% d-phenothrin) was also tested as a comparison. Our results showed that the knockdown effect of the commercial aerosol spray and P. aduncum essential oil spray (8% and 10% concentrations) was significantly higher in Ae. albopictus adult females, when compared with that of Ae. aegypti adult females (P<0.05). There was a significant difference in knockdown between commercial aerosol spray and essential oil spray for both Aedes spp. (P<0.05). The essential oil induced significantly higher mortality in Ae. aegypti (80%) than in Ae. albopictus (71.6%) (P<0.05). The commercial aerosol spray caused 97.7% and 86.5% mortality against Ae. aegypti and Ae. albopictus respectively (P<0.05). Based on these data, P. aduncum essential oil has the potential to be used as an aerosol spray against Aedes spp.

  3. Effects of insemination and blood-feeding on locomotor activity of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) females under laboratory conditions

    OpenAIRE

    Lima-Camara, Tamara N; Lima, José B P; Bruno, Rafaela V; Peixoto, Alexandre A

    2014-01-01

    Abstract Background Dengue is an arbovirus disease transmitted by two Aedes mosquitoes: Ae. aegypti and Ae. albopictus. Virgin females of these two species generally show a bimodal and diurnal pattern of activity, with early morning and late afternoon peaks. Although some studies on the flight activity of virgin, inseminated and blood-fed Ae. aegypti females have been carried out under laboratory conditions, little...

  4. Estimates of public health risks through import of Aedes albopictus via Dracaena's in the Netherlands : Investigation in the establlishment of Aedes albopictus : final report

    NARCIS (Netherlands)

    Takken, W.; Jacobs, F.H.H.

    2008-01-01

    Between September 2005 and October 2007 studies were done in six horticultural companies in the Netherlands to assess the presence of the exotic Aedes albopictus and to determine whether the species had become established. Adult mosquitoes were sampled with liberty plus carbon dioxide traps. The

  5. Comparison of BG-Sentinel® Trap and Oviposition Cups for Aedes aegypti and Aedes albopictus Surveillance in Jacksonville, Florida, USA.

    Science.gov (United States)

    Wright, Jennifer A; Larson, Ryan T; Richardson, Alec G; Cote, Noel M; Stoops, Craig A; Clark, Marah; Obenauer, Peter J

    2015-03-01

    The BG-Sentinel® (BGS) trap and oviposition cups (OCs) have both proven effective in the surveillance of Aedes species. This study aimed to determine which of the 2 traps could best characterize the relative population sizes of Aedes albopictus and Aedes aegypti in an urban section of Jacksonville, FL. Until 1986, Ae. aegypti was considered the dominant container-breeding species in urban northeastern Florida. Since the introduction of Ae. albopictus, Ae. aegypti has become almost completely extirpated. In 2011, a resurgence of Ae. aegypti was detected in the urban areas of Jacksonville; thus this study initially set out to determine the extent of Ae. aegypti reintroduction to the area. We determined that the BGS captured a greater number of adult Ae. aegypti than Ae. albopictus, while OCs did not monitor significantly different numbers of either species, even in areas where the BGS traps suggested a predominance of one species over the other. Both traps were effective at detecting Aedes spp.; however, the BGS proved more diverse by detecting over 20 other species as well. Our results show that in order to accurately determine vectorborne disease threats and the impact of control operations on these 2 species, multiple trapping techniques should be utilized when studying Ae. aegypti and Ae. albopictus population dynamics.

  6. Hallazgo de mesocyclops aspericornis (Daday) (Copepoda: Cyclopidae) depredador de larvas de aedes aegypti en Anapoima Colombia (1)

    OpenAIRE

    Marco Fidel Suárez; Dwight Ayala; Michael J. Nelson; Janet W. Reid

    1984-01-01

    En Anapoima, Colombia, se encontró que el copépodo Mesocyclops aspericornis era depredador de larvas del mosquito Aedes aegypti. Este encuentro representa el primer hallazgo de este copépodo en recipientes artificiales en la región neotropical, y el primer hallazgo como depredador de larvas de Aedes aegypti.

  7. Analogy in causal inference: rethinking Austin Bradford Hill's neglected consideration.

    Science.gov (United States)

    Weed, Douglas L

    2018-05-01

    The purpose of this article was to rethink and resurrect Austin Bradford Hill's "criterion" of analogy as an important consideration in causal inference. In epidemiology today, analogy is either completely ignored (e.g., in many textbooks), or equated with biologic plausibility or coherence, or aligned with the scientist's imagination. None of these examples, however, captures Hill's description of analogy. His words suggest that there may be something gained by contrasting two bodies of evidence, one from an established causal relationship, the other not. Coupled with developments in the methods of systematic assessments of evidence-including but not limited to meta-analysis-analogy can be restructured as a key component in causal inference. This new approach will require that a collection-a library-of known cases of causal inference (i.e., bodies of evidence involving established causal relationships) be developed. This library would likely include causal assessments by organizations such as the International Agency for Research on Cancer, the National Toxicology Program, and the United States Environmental Protection Agency. In addition, a process for describing key features of a causal relationship would need to be developed along with what will be considered paradigm cases of causation. Finally, it will be important to develop ways to objectively compare a "new" body of evidence with the relevant paradigm case of causation. Analogy, along with all other existing methods and causal considerations, may improve our ability to identify causal relationships. Copyright © 2018 Elsevier Inc. All rights reserved.

  8. Rainwater harvesting potential sites at margalla hills national park

    International Nuclear Information System (INIS)

    Khalid, B.; Mushtaq, N.; Sial, M.

    2013-01-01

    Life without water is not possible. Adoption of modern lifestyle and increase in population is leading to a water scarce world. The demand of world population cannot be met , which is resulting in increased groundwater abstraction. The world is facing water crisis and Pakistan is no exception. Urban areas of Pakistan are affected badly where extraction is higher while the construction of pavements has disturbed groundwater infiltration. The Federal Capital of Pakistan, Islamabad, is located in Pothohar region of the country and faces severe water shortages, particularly during summers. Extensive drilling by public and private users lowers groundwater table. Satellite imagery of LANDSAT 7 ETM+ and ASTER DEM 30m resolution were used to construct the site suitability map for groundwater recharge of Margalla Hills National Park. Factors considered included land cover, drainage density, elevation and slope. Suitable weight ages were assigned to these factors according to their influence on infiltration in the study area. Groundwater recharge at Margalla Hills National Park will be effective in dealing with water crisis in Islamabad as it will raise groundwater table of the adjacent areas. (author)

  9. Woods and Russell, Hill, and the emergence of medical statistics.

    Science.gov (United States)

    Farewell, Vern; Johnson, Tony

    2010-06-30

    In 1937, Austin Bradford Hill wrote Principles of Medical Statistics (Lancet: London, 1937) that became renowned throughout the world and is widely associated with the birth of modern medical statistics. Some 6 years earlier Hilda Mary Woods and William Thomas Russell, colleagues of Hill at the London School of Hygiene and Tropical Medicine, wrote a similar book An Introduction to Medical Statistics (PS King and Son: London, 1931) that is little known today. We trace the origins of these two books from the foundations of early demography and vital statistics, and make a detailed examination of some of their chapters. It is clear that these texts mark a watershed in the history of medical statistics that demarcates the vital statistics of the nineteenth and early twentieth centuries from the modern discipline. Moreover, we consider that the book by Woods and Russell is of some importance in the development of medical statistics and we describe and acknowledge their place in the history of this discipline. (c) 2010 John Wiley & Sons, Ltd.

  10. Vertical distribution of Aedes mosquitoes in multiple storey buildings in Selangor and Kuala Lumpur, Malaysia.

    Science.gov (United States)

    Lau, K W; Chen, C D; Lee, H L; Izzul, A A; Asri-Isa, M; Zulfadli, M; Sofian-Azirun, M

    2013-03-01

    The aim of the present study was to determine the vertical distribution and abundance of Aedes mosquitoes in multiple storey buildings in Selangor and Kuala Lumpur, Malaysia. Ovitrap surveillance was conducted for 4 continuous weeks in multiple storey buildings in 4 residential areas located in Selangor [Kg. Baiduri (KB)] and Kuala Lumpur [Student Hostel of University of Malaya (UM), Kg. Kerinchi (KK) and Hang Tuah (HT)]. The results implied that Aedes mosquitoes could be found from ground floor to highest floor of multiple storey buildings and data from different elevation did not show significant difference. Ovitrap index for UM, KB, HT and KK ranged from 0 - 29.17%, 0 - 55.56%, 8.33 - 83.33% and 0 - 91.17% respectively. Aedes aegypti and Aedes albopictus were found breeding in HT, KK and KB; while only Ae. albopictus was obtained from UM. The results indicate that the invasion of Aedes mosquitoes in high-rise apartments could facilitate the transmission of dengue virus and new approaches to vector control in this type of residential area should be developed.

  11. Investigation of the Cry4B-prohibitin interaction in Aedes aegypti cells.

    Science.gov (United States)

    Kuadkitkan, Atichat; Smith, Duncan R; Berry, Colin

    2012-10-01

    Bacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, and chikungunya. Previous work has shown that Cry4B binds to several mid-gut membrane proteins in Aedes aegypti larvae including prohibitin, a protein recently identified as a receptor that also mediates entry of dengue virus into Aedes cells. This study confirms the interaction between Cry4B and prohibitin by co-immunoprecipitation analysis and demonstrates colocalization of prohibitin and Cry4B by confocal microscopy. While activated Cry4B toxin showed high larvicidal activity, it was not cytotoxic to two Aedes cell lines, allowing determination of its effect on dengue virus infectivity in the absence of Cry4B-induced cell lysis. Pre-exposure of Aedes cells to Cry4B resulted in a significant reduction in the number of infected cells compared to untreated cells.

  12. Hubungan keberadaan larva nyamuk Aedes Spp dengan kasus Demam Berdarah Dengue di Kota Bandung

    Directory of Open Access Journals (Sweden)

    Lukman Hakim

    2015-12-01

    Full Text Available Abstract. Bandung is the region with most cases of dengue hemorrhagic fever (DHF in West Java with 24,491 cases in the period 2009-2013. Dengue virus transmission occurs from DHF patients through the bite of Aedes aegypti and Ae. albopictus as the primary vector, while Ae. polynesiensis, Ae. scutellaris and Ae. (Finlayaniveus as secondary vectors. To determine the relationship of the presence of larvae of Aedes spp with DHF, has conducted research with larval survey and analysis of dengue cases in the city of Bandung. Research was carried out by recording DHF patients in the period 2011-2013, then visit to interview and survey of Aedes mosquito larvae in water containers inside and outside the home. Samples were successfully visited are 402 houses consist of 201 houses with DHF cases and 201 without DHF cases. The results showed that of 402 samples were obtained 75 positive of larvae of Aedes spp (house index/HI 18.7% consisted of 36 without DHF patient and 39 with DHF patient. Among the 8 villages research sites, highest HI is Cijaura village is 21.9%, and the lowest is village Manjahlega (11.1% (9 houses, while in the Cidurian village was  not found Aedes mosquitoes larvae. The most number of houses which positive of larvae of Aedes spp water containers are Sekejati village (37 houses, whereas the least was the Manjahlega village (2 houses. The results of chi square analysis and correlation, showed that there was no significant association between the presence of larvae of Aedes spp with DHF cases. Concluded, the presence of larvae of Aedes spp not significant associated with DHF cases in Bandung City, West Java. For the occurrence of dengue cases, beside the presence of Aedes spp, is also influenced by other factors such as the vector capacity, dengue virus virulence and host immune status. We recommended, to DHF control, beside conducting the control of dengue vector, is also needs to be carried out the other activities related to the

  13. Distribution of Aedes mosquitoes in the Kilimanjaro Region of northern Tanzania.

    Science.gov (United States)

    Hertz, Julian T; Lyaruu, Lucille J; Ooi, Eng Eong; Mosha, Franklin W; Crump, John A

    2016-05-01

    Little is known about the presence and distribution of Aedes mosquitoes in northern Tanzania despite the occurence of viruses transmitted by these mosquitoes such as Chikungunya virus (CHIKV) and Dengue virus (DENV) in the region. Adult and larval mosquitoes were collected from rural and urban settings across a wide range of altitudes in the Kilimanjaro Region using the Mosquito Magnet CO2 Trap for collection of adults and old tires for breeding of larvae. Polymerase chain reaction assays were performed on captured adult mosquitoes to detect the presence of CHIKV and DENV. A total of 2609 Aedes aegypti adult mosquitoes were collected; no other Aedes species larvae were found. Mosquito yields were significantly higher in urban settings than rural settings (26.5 vs. 1.9 mosquitoes per day, p = 0.037). A total of 6570 Ae. aegypti larvae were collected from old tires; no other Aedes species larvae were found. Of the 2609 adult mosquitoes collected, none tested positive for CHIKV or DENV. As far as we are aware, this paper reports for the first time the presence of Ae. aegypti in the Kilimanjaro Region of northern Tanzania. Although CHIKV and DENV were not isolated from any of the collected mosquitoes in this study, the apparent absence of other Aedes species in the area suggests that Ae. aegypti is the primary local vector of these infections.

  14. Systematic Review: Land Cover, Meteorological, and Socioeconomic Determinants of Aedes Mosquito Habitat for Risk Mapping

    Directory of Open Access Journals (Sweden)

    Mohamed F. Sallam

    2017-10-01

    Full Text Available Asian tiger and yellow fever mosquitoes (Aedes albopictus and Ae. aegypti are global nuisances and are competent vectors for viruses such as Chikungunya (CHIKV, Dengue (DV, and Zika (ZIKV. This review aims to analyze available spatiotemporal distribution models of Aedes mosquitoes and their influential factors. A combination of five sets of 3–5 keywords were used to retrieve all relevant published models. Five electronic search databases were used: PubMed, MEDLINE, EMBASE, Scopus, and Google Scholar through 17 May 2017. We generated a hierarchical decision tree for article selection. We identified 21 relevant published studies that highlight different combinations of methodologies, models and influential factors. Only a few studies adopted a comprehensive approach highlighting the interaction between environmental, socioeconomic, meteorological and topographic systems. The selected articles showed inconsistent findings in terms of number and type of influential factors affecting the distribution of Aedes vectors, which is most likely attributed to: (i limited availability of high-resolution data for physical variables, (ii variation in sampling methods; Aedes feeding and oviposition behavior; (iii data collinearity and statistical distribution of observed data. This review highlights the need and sets the stage for a rigorous multi-system modeling approach to improve our knowledge about Aedes presence/abundance within their flight range in response to the interaction between environmental, socioeconomic, and meteorological systems.

  15. Potensi Tanaman Di Indonesia Sebagai Larvasida Alami Untuk Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Yoke Astriani

    2017-02-01

    Full Text Available AbstractDengue Hemorrhagic Fever (DHF still become one of the health problems in Indonesia. This disease has emerged since 1968 and has become one of the deadliest disease in various regions in Indonesia. Indonesia has not implemented an effective dengue vaccine program to provide protection against all four serotypes of the dengue virus. Vector control using chemical insecticides has made the vectors resistant to the insectisides. One option to avoid those bad effect is natural larvicide. The lack of literature that can be used as a foundation for further studies on the natural larvicidal is the reason why a review of several articles and research results is needed. We use the method of literature review. Our literature reveals that 68% of the 25 plants species are categorized as highly effective with LC50 <750 ppm. Jasmine, Zodia and Tobacco have the highest effectivity compared to the other plants use in this literature review. The LC50 of these plants are 0.999 ppm, 1.94 ppm, and 1.94 ppmrespectively. All twenty five plants that we present suitable to be cultivated in the region of Indonesia with tropical climate so that people can easily cultivate and use it as a natural larvicides.Keyword: Natural larvicides, Aedes aegypti, LC50, and essential oilAbstrakPenyakit Demam Berdarah Dengue (DBD sampai saat ini masih menjadi salah satu permasalahan dunia kesehatan di Indonesia. Penyakit yang muncul sejak tahun 1968 ini telah banyak menelan korban di berbagai daerah dan hingga saat ini, Indonesia belum menerapkan program vaksin yang efektif untuk memberikan perlindungan terhadap empat serotipe dari virus dengue. Pengendalian vektor menggunakan bahan insektisida kimiawi banyak memberikan efek resisten terhadap insektisida tersebut. Salah satu pilihan untuk menghindari hal tersebut dibutuhkan adanya larvasida alami. Oleh karena masih tersebarnya informasi yang dapat dijadikan dasar untuk studi lanjut mengenai larvasida alami, maka tulisan ini

  16. Terraplenes sobre suelos colapsables

    OpenAIRE

    Arnedo Gaute, Diego

    2004-01-01

    Esta tesina está motivada por el diseño y construcción del Canal Segarra-Garrigues. El trazado del canal atraviesa varios pequeños valles de fondo llano mediante terraplenes sobre los cuales circula el canal. En estas vaguadas se encuentran suelos limo-arcillosos de baja densidad cuya potencia puede llegar a ser de varios metros. Estos materiales, debido a su estructura abierta, son potencialmente colapsables una vez cargados si experimentan incrementos de humedad. Las deformacion...

  17. DISCUTINDO SOBRE SEXTING

    Directory of Open Access Journals (Sweden)

    Suzana da Conceição de Barros

    2013-07-01

    Full Text Available Na contemporaneidade, os sujeitos vêm se utilizando das diversas tecnologias digitais, para exporem e compartilharem sua intimidade, com intuito de seduzirem e tornarem-se visíveis. Esse fenômeno vem sendo chamado de sexting, e vem sendo aderido por sujeitos de várias faixas etárias. Assim, leitor/a, convido você a pensar sobre o sexting e o que a escola tem a ver com essa prática?

  18. Excertos sobre o sedentarismo

    OpenAIRE

    Palma,Alexandre; Vilaça,Murilo Mariano; Assis,Monique Ribeiro de

    2014-01-01

    O sedentarismo tem sido tratado como algo perfeitamente determinável, objetivo e, portanto, que pode ser apreendido. Por outro lado, o conceito de sedentarismo ainda permanece questionável e, por conseguinte, os instrumentos mostram-se inconsistentes. Neste sentido, o objetivo do presente estudo foi mostrar que diferentes discursos de verdade vêm concorrendo por esse conceito, o que nos motiva a pensar qual é a verdade ou, até mesmo, se há alguma verdade sobre ele. Após apresentarmos diferent...

  19. MANEJO DE RESIDUOS DE Eucalyptus grandis Hill ex Maiden EN SITIOS CONTRASTANTES

    Directory of Open Access Journals (Sweden)

    Ana Maria Lupi

    2017-01-01

    Full Text Available En la región NE de Argentina existe poca información sobre el impacto que tienen las diferentes alternativas de manejo de residuos de la cosecha forestal sobre las propiedades edáficas. En este estudio se analizó el efecto a corto plazo de sistemas de manejo de residuos de la cosecha de Eucalyptus grandis Hill ex Maiden, sobre la concentración del carbono orgánico total del suelo (COT y la fracción particulada (COP, su estado estructural y la conductividad hidráulica saturada. El estudio se desarrolló en dos sitios de la Provincia de Entre Ríos, Argentina, en un suelo Aquic Hapluderts (S1 y en un Fluventic Hapludolls (S2. Se aplicaron los siguientes tratamientos: 1. Extracción total de residuos (ER, 2. Conservación de residuos (CR, 3. Quema de residuos-testigo (QR, 4. Doble adición de residuos (DAR. El muestreo de suelos en las profundidades 0-5 cm y 5-10 cm se realizó a los 18 (S1 y a los 22 (S2 meses de aplicar los tratamientos. En el corto plazo, el efecto de los tratamientos dependió del tipo de suelo y de la variable analizada. Los contrastes más importantes se debieron al efecto de las características intrínsecas del suelo sobre el tamaño de los agregados y la conductividad hidráulica saturada. La conservación de residuos no aumentó la concentración de COT ni modificó la calidad de la materia orgánica medida a partir de COP. El suelo Aquic Hapluderts presentó agregados más grandes, más estables y una mayor K sat en comparación con el Fluventic Hapludolls. En el Aquic Hapluderts se observó una mayor conductividad hidráulica saturada en los tratamientos con conservación de los residuos (DAR y CR, debido a que la cobertura preservaría el sistema poroso en la interfase suelo-atmosfera. El Fluventic Hapludolls fue menos estable y los tratamientos no modificaron significativamente el tamaño de los agregados ni la conductividad hidráulica saturada . Considerando que las características del sitio tienen efectos

  20. Conservation assessment for the autumn willow in the Black Hills National Forest, South Dakota and Wyoming

    Science.gov (United States)

    J. Hope Hornbeck; Carolyn Hull Sieg; Deanna J. Reyher

    2003-01-01

    Autumn willow, Salix serissima (Bailey) Fern., is an obligate wetland shrub that occurs in fens and bogs in the northeastern United States and eastern Canada. Disjunct populations of autumn willow occur in the Black Hills of South Dakota. Only two populations occur on Black Hills National Forest lands: a large population at McIntosh Fen and a small...

  1. 77 FR 33560 - Southwest Pennsylvania Railroad Company-Acquisition Exemption-Laurel Hill Development Corporation

    Science.gov (United States)

    2012-06-06

    ... Pennsylvania Railroad Company--Acquisition Exemption-- Laurel Hill Development Corporation Southwest... 49 CFR 1150.41 to acquire a 0.66-mile line of railroad owned by Laurel Hill Development Corporation... rail line. Most recently, in Southwest Pennsylvania Railroad Company--Acquisition Exemption--Laurel...

  2. 77 FR 2128 - Southwest Pennsylvania Railroad Company-Acquisition Exemption-Laurel Hill Development Corporation

    Science.gov (United States)

    2012-01-13

    ... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [Docket No. FD 35584] Southwest Pennsylvania Railroad Company--Acquisition Exemption-- Laurel Hill Development Corporation Southwest... 49 CFR 1150.41 to acquire a number of rail lines now owned by Laurel Hill Development Corporation...

  3. Hume, Mill, Hill, and the sui generis epidemiologic approach to causal inference.

    Science.gov (United States)

    Morabia, Alfredo

    2013-11-15

    The epidemiologic approach to causal inference (i.e., Hill's viewpoints) consists of evaluating potential causes from the following 2, noncumulative angles: 1) established results from comparative, observational, or experimental epidemiologic studies; and 2) reviews of nonepidemiologic evidence. It does not involve statements of statistical significance. The philosophical roots of Hill's viewpoints are unknown. Superficially, they seem to descend from the ideas of Hume and Mill. Hill's viewpoints, however, use a different kind of evidence and have different purposes than do Hume's rules or Mill's system of logic. In a nutshell, Hume ignores comparative evidence central to Hill's viewpoints. Mill's logic disqualifies as invalid nonexperimental evidence, which forms the bulk of epidemiologic findings reviewed from Hill's viewpoints. The approaches by Hume and Mill cannot corroborate successful implementations of Hill's viewpoints. Besides Hume and Mill, the epidemiologic literature is clueless about a plausible, pre-1965 philosophical origin of Hill's viewpoints. Thus, Hill's viewpoints may be philosophically novel, sui generis, still waiting to be validated and justified.

  4. Sharing the Gift of Jazz: An Interview with Willie L. Hill Jr.

    Science.gov (United States)

    Howe, Brad

    2011-01-01

    This article presents an interview with Willie L. Hill Jr., founder and director of the Society for Jazz Education. Currently a professor of music education at the University of Massachusetts-Amherst and the director of the UMass Fine Arts Center, Hill has served as director of education for the Thelonious Monk Institute of Jazz. He is a past…

  5. Rare Plants and Animals of the Texas Hill Country: Educator's Guide.

    Science.gov (United States)

    Texas State Dept. of Parks and Wildlife, Austin.

    Texas Hill Country is a land of fresh water springs, stony hills, and steep canyons and home to many rare plants and animals. Six activities for grades 3-5 and six activities for grades 6-12 are contained in this guide. Elementary activity highlights include using "The Lorax" by Dr. Seuss to stimulate critical thinking about…

  6. The Hill's three-body problem: a new deduction of motion equations

    International Nuclear Information System (INIS)

    Fuente Marcos, C. de la.

    1995-01-01

    Although the Hill's problem has been considered traditionally as a particular case of the restricted problem, it is not but rather a different problem with the same degree of generality. In this paper it is presented a new derivation of the motion equations obtained originally by Hill in 1878 as well as a brief discussion about its possible applications. (Author) 13 refs

  7. Leaking Containers: Success and Failure in Controlling the Mosquito Aedes aegypti in Brazil.

    Science.gov (United States)

    Löwy, Ilana

    2017-04-01

    In 1958, the Pan American Health Organization declared that Brazil had successfully eradicated the mosquito Aedes aegypti, responsible for the transmission of yellow fever, dengue fever, chikungunya, and Zika virus. Yet in 2016 the Brazilian minister of health described the situation of dengue fever as "catastrophic." Discussing the recent epidemic of Zika virus, which amplified the crisis produced by the persistence of dengue fever, Brazil's president declared in January 2016 that "we are in the process of losing the war against the mosquito Aedes aegypti." I discuss the reasons for the failure to contain Aedes in Brazil and the consequences of this failure. A longue durée perspective favors a view of the Zika epidemic that does not present it as a health crisis to be contained with a technical solution alone but as a pathology that has the persistence of deeply entrenched structural problems and vulnerabilities.

  8. PENGAMATAN TEMPAT PERINDUKAN AEDES AEGYPTI PADA TEMPAT PENAMPUNGAN AIR RUMAH TANGGA PADA MASYARAKAT PENGGUNA AIR OLAHAN

    Directory of Open Access Journals (Sweden)

    H. Hasyimi

    2012-11-01

    Full Text Available An observation of Aedes aegypti breeding places in domestic pipe waters container provided by PAM (Water supply company customer regency was carried out. The area observation in RW (a hamlet 05 kelurahan Papanggo Tanjung priuk North Jakarta. As a control area, RW 04 kelurahan Tanjung Priok in the same district was selected. The observation was conducted on Agust - September 2001. The result showed that Aedes aegypti larvae were found mostly in clay water container or tempayan (66,7 %. The house index (HI rate is 27,3%. In the control area the larvae were found predominantly in bath cistern (65,4% and HI rate is 100%. So in the study area HI rate is lower than in the control area.   Keywords: Aedes aegypti, breeding places, domestic container, house index

  9. Penyelesaian Masalah 8-Puzzle dengan Algoritma Steepest-Ascent Hill Climbing

    Directory of Open Access Journals (Sweden)

    David Abraham

    2016-03-01

    Full Text Available 8 puzzle merupakan salah satu implementasi dari Artificial Intelegence. Dalam proses penyelesaiannya banyak terdapat algoritma-algoritma pencarian yang dapat diterapkan. Solusi 8 puzzle akan lebih cepat diperoleh jika digunakan prinsip array dengan variasi algoritma Steepest-Ascent Hill Climbing (Hill Climbing dengan memilih kemiringan yang paling tajam / curam dengan parameter heuristik posisi yang benar dan heuristik jarak serta dikombinasikan dengan LogList sebagai penyimpanan state state yang pernah dilalui untuk menanggulangi permasalah pada algoritma hill climbing itu sendiri dan terhindar dari looping state yang pernah dilalui. Metode-metode yang termasuk ke dalam teknik pencarian yang berdasarkan pada fungsi heuristik salah satu diantaranya adalah Hill Climbing, Best First Search, A* (A Bintang. Loglist merupakan tempat penyimpanan setiap kunjungan dari state-state puzzle yang telah dilakukan untuk menghindari looping atau pengulangan terhadap state yang pernah dilalui. Untuk menanggulangi permasalahan pada SteepestAscent Hill Climbing.

  10. Leucemias y radiación: juicio causal según los criterios de SIR Austin Bradford Hill

    Directory of Open Access Journals (Sweden)

    Octavio Martínez Betancur

    2010-07-01

    Full Text Available El cometido del presente trabajo es la lectura interpretativa con base en la propuesta de inferencia causal de Austin Bradford Hill, del documento Radiation-related leukemia in Hiroshima and Nagasaki, 1946-1964. I. Distribution, incidence and appearance time. El documento informado por el comité de las víctimas de la bomba atómica cumple con la totalidad de los nueve “criterios” propuesto por Hill, y entre las limitaciones se cuentan la presunciones de homogeneidad genética de los sujetos expuestos y linealidad de los efectos de las radiaciones en la leucemia, además del hecho de sólo considerar la edad como variable confusora. Por otra parte, el número de muertes por leucemia, patrón de oro de la sensibilidad y la especificidad del sistema de vigilancia del estudio de duración de la vida, fue 61 muertes por leucemia entre los supervivientes situados dentro de 1500 metros del epicentro de la explosión y 25 muertes en el grupo que recibió radiación estando entre 1501 y 10000 metros, lo que habla de posible subregistro de casos y de sobrediagnóstico por la sumatoria de criterios empleados. Los “criterios” de Hill no son reglas lógicas sino metodológicas, que ayudan en el proceso de decisión sobre la inferencia causal en epidemiología. Se apartan de la discusión de si las inferencias a partir de ellos se ajustan a la lógica inductiva o a la deductiva, y deben entenderse pragmáticamente como “inferencias de la mejor explicación” en el contexto de la abducción. La única conclusión permitida dentro del marco de la “inferencia de la mejor explicación”, es que se tienen buenas razones para considerar seriamente la hipótesis que la asociación estadística que se estudia, es una relación causal.

  11. Sobre historia mundial hoy

    Directory of Open Access Journals (Sweden)

    Vera Weiler

    2001-01-01

    Full Text Available Los procesos de globalización son una realidad; su enorme impacto sobre la vida humana los ha convertido en un tema recurrente sobre el que se escribe y se comenta a diario también en Colombia. Los libros que de ellos tratan, ante todo los que 10 hacen en tono crítico, baten records en la industria editorial en todo el mundo. Las expectativas que acerca del futuro abrigan cientos de millones de personas se relacionan con 10 que ellas esperan, para bien o para mal, de la globalización. Las posturas que los gobernantes ocupan al respecto en el mundo son, cuando menos de aceptación, generalmente de activa participación en pos de la globalización. Por un lado, crece la preocupación y se multiplican las protestas; por el otro, dominan los razonamientos de los especialistas en materia de maximización de los rendimientos de los capitales que presentan la globalización, al estilo que se viene imponiendo, como el camino ineludible del gobierno universal de la eficiencia. ¿Tienen que decir algo los historiadores frente a todo esto?.

  12. Citrus Seed Oils Efficacy against Larvae of Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Hazrat Bilal

    2017-10-01

    Full Text Available Background: Dengue fever is a serious public health issue in Pakistan for many years. Globally plants have been reported to contain compounds with insecticidal properties. These properties have been demonstrated more recently on the larval stages of mosquitoes. Therefore, Citrus cultivar seeds were evaluated for larvicidal potential against the primary dengue vector Aedes aegypti.Methods: Extraction of oil was done by a steam distillation method and oils were evaluated according to WHO guidelines for larvicides 2005 for evaluation of insecticidal properties of citrus seed extracts against mosquito larvae.Result: Among the Citrus cultivar seed oil, rough lemon (Citrus jambhiri had the lowest LC50 value (200.79ppm, while musambi (C. sinensis var musambi had the highest LC50 value (457.30ppm after 24 h of exposure.Conclusion: Citrus cultivars have some larvicidal potential but C. jambhiri had the greatest potential against A. ae­gypti larvae. Further small-scale field trials using the extracts of C. jambhiri will be conducted to determine opera­tional feasibility.

  13. Insights into the transcriptome of oenocytes from Aedes aegypti pupae

    Directory of Open Access Journals (Sweden)

    Gustavo Ferreira Martins

    2011-05-01

    Full Text Available Oenocytes are ectodermic cells present in the fat body of several insect species and these cells are considered to be analogous to the mammalian liver, based on their role in lipid storage, metabolism and secretion. Although oenocytes were identified over a century ago, little is known about their messenger RNA expression profiles. In this study, we investigated the transcriptome of Aedes aegypti oenocytes. We constructed a cDNA library from Ae. aegypti MOYO-R strain oenocytes collected from pupae and randomly sequenced 687 clones. After sequences editing and assembly, 326 high-quality contigs were generated. The most abundant transcripts identified corresponded to the cytochrome P450 superfamily, whose members have roles primarily related to detoxification and lipid metabolism. In addition, we identified 18 other transcripts with putative functions associated with lipid metabolism. One such transcript, a fatty acid synthase, is highly represented in the cDNA library of oenocytes. Moreover, oenocytes expressed several immunity-related genes and the majority of these genes were lysozymes. The transcriptional profile suggests that oenocytes play diverse roles, such as detoxification and lipid metabolism, and increase our understanding of the importance of oenocytes in Ae. aegypti homeostasis and immune competence.

  14. Larvicidal activity against Aedes aegypti of pacharin from Bauhinia acuruana.

    Science.gov (United States)

    da Silva Góis, Roberto Wagner; de Sousa, Leôncio Mesquita; Santiago, Gilvandete Maria Pinheiro; Romero, Nirla Rodrigues; Lemos, Telma Leda Gomes; Arriaga, Angela Martha Campos; Braz-Filho, Raimundo

    2013-07-01

    The aim of the present study was to evaluate the activity of pacharin isolated from the ethanol extract from roots of Bauhinia acuruana on third-instar larvae of Aedes aegypti Linn. (Diptera: Culicidae). The crude ethanol extract showed larvicidal activity at the concentration of 500 μg/mL. Given this larvicidal activity, this extract was submitted to chromatographic fractionation on a silica gel column eluted with n-hexane, dichloromethane, ethyl ether, ethyl acetate, and methanol in order to isolate the active compound(s). Pacharin, obtained in pure form from fraction eluted with ethyl ether, was evaluated for their larvicidal effects against A. aegypti. In these bioassays, the larvae were exposed at concentrations of 500, 250, 100, 50, and 25 μg/mL of the crude ethanol extract or pacharin. After 24 h, the number of dead larvae was counted and the LC₅₀ values for larval mortality were calculated. Pacharin showed LC50 value of 78.9 ± 1.8 μg/mL. The structure of isolated compound was identified on the basis of their spectral data (IR, 1D- and 2D-NMR) and by comparison with literature spectral data. The results indicate pacharin as a potential natural larvicide.

  15. The Aedes aegypti toll pathway controls dengue virus infection.

    Directory of Open Access Journals (Sweden)

    Zhiyong Xi

    2008-07-01

    Full Text Available Aedes aegypti, the mosquito vector of dengue viruses, utilizes its innate immune system to ward off a variety of pathogens, some of which can cause disease in humans. To date, the features of insects' innate immune defenses against viruses have mainly been studied in the fruit fly Drosophila melanogaster, which appears to utilize different immune pathways against different types of viruses, in addition to an RNA interference-based defense system. We have used the recently released whole-genome sequence of the Ae. aegypti mosquito, in combination with high-throughput gene expression and RNA interference (RNAi-based reverse genetic analyses, to characterize its response to dengue virus infection in different body compartments. We have further addressed the impact of the mosquito's endogenous microbial flora on virus infection. Our findings indicate a significant role for the Toll pathway in regulating resistance to dengue virus, as indicated by an infection-responsive regulation and functional assessment of several Toll pathway-associated genes. We have also shown that the mosquito's natural microbiota play a role in modulating the dengue virus infection, possibly through basal-level stimulation of the Toll immune pathway.

  16. Germline excision of transgenes in Aedes aegypti by homing endonucleases.

    Science.gov (United States)

    Aryan, Azadeh; Anderson, Michelle A E; Myles, Kevin M; Adelman, Zach N

    2013-01-01

    Aedes (Ae.) aegypti is the primary vector for dengue viruses (serotypes1-4) and chikungunya virus. Homing endonucleases (HEs) are ancient selfish elements that catalyze double-stranded DNA breaks (DSB) in a highly specific manner. In this report, we show that the HEs Y2-I-AniI, I-CreI and I-SceI are all capable of catalyzing the excision of genomic segments from the Ae. aegypti genome in a heritable manner. Y2-I-AniI demonstrated the highest efficiency at two independent genomic targets, with 20-40% of Y2-I-AniI-treated individuals producing offspring that had lost the target transgene. HE-induced DSBs were found to be repaired via the single-strand annealing (SSA) and non-homologous end-joining (NHEJ) pathways in a manner dependent on the availability of direct repeat sequences in the transgene. These results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, and confirm the utility of HEs in the manipulation and modification of transgenes in this important vector.

  17. Dengue virus replicates and accumulates in Aedes aegypti salivary glands

    Energy Technology Data Exchange (ETDEWEB)

    Raquin, Vincent, E-mail: vincent.raquin@univ-lyon1.fr [Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris (France); Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, 75015 Paris (France); Lambrechts, Louis, E-mail: louis.lambrechts@pasteur.fr [Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris (France); Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, 75015 Paris (France)

    2017-07-15

    Dengue virus (DENV) is an RNA virus transmitted among humans by mosquito vectors, mainly Aedes aegypti. DENV transmission requires viral dissemination from the mosquito midgut to the salivary glands. During this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. Despite the implications for virus transmission and evolution, DENV replication in salivary glands has not been directly demonstrated. Here, we used a strand-specific quantitative RT-PCR assay to demonstrate that negative-strand DENV RNA is produced in Ae. aegypti salivary glands, providing conclusive evidence that viral replication occurs in this tissue. Furthermore, we showed that the concentration of DENV genomic RNA in salivary glands increases significantly over time, indicating that active replication likely replenishes DENV genetic diversity prior to transmission. These findings improve our understanding of the biological determinants of DENV fitness and evolution. - Highlights: •Strand-specific RT-qPCR allows accurate quantification of DENV (-) RNA in mosquito tissues. •Detection of DENV (-) RNA in salivary glands provides evidence of viral replication in this tissue. •Viral replication in salivary glands likely replenishes DENV genetic diversity prior to transmission.

  18. Dengue virus replicates and accumulates in Aedes aegypti salivary glands

    International Nuclear Information System (INIS)

    Raquin, Vincent; Lambrechts, Louis

    2017-01-01

    Dengue virus (DENV) is an RNA virus transmitted among humans by mosquito vectors, mainly Aedes aegypti. DENV transmission requires viral dissemination from the mosquito midgut to the salivary glands. During this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. Despite the implications for virus transmission and evolution, DENV replication in salivary glands has not been directly demonstrated. Here, we used a strand-specific quantitative RT-PCR assay to demonstrate that negative-strand DENV RNA is produced in Ae. aegypti salivary glands, providing conclusive evidence that viral replication occurs in this tissue. Furthermore, we showed that the concentration of DENV genomic RNA in salivary glands increases significantly over time, indicating that active replication likely replenishes DENV genetic diversity prior to transmission. These findings improve our understanding of the biological determinants of DENV fitness and evolution. - Highlights: •Strand-specific RT-qPCR allows accurate quantification of DENV (-) RNA in mosquito tissues. •Detection of DENV (-) RNA in salivary glands provides evidence of viral replication in this tissue. •Viral replication in salivary glands likely replenishes DENV genetic diversity prior to transmission.

  19. Modulation of Host Learning in Aedes aegypti Mosquitoes.

    Science.gov (United States)

    Vinauger, Clément; Lahondère, Chloé; Wolff, Gabriella H; Locke, Lauren T; Liaw, Jessica E; Parrish, Jay Z; Akbari, Omar S; Dickinson, Michael H; Riffell, Jeffrey A

    2018-02-05

    How mosquitoes determine which individuals to bite has important epidemiological consequences. This choice is not random; most mosquitoes specialize in one or a few vertebrate host species, and some individuals in a host population are preferred over others. Mosquitoes will also blood feed from other hosts when their preferred is no longer abundant, but the mechanisms mediating these shifts between hosts, and preferences for certain individuals within a host species, remain unclear. Here, we show that olfactory learning may contribute to Aedes aegypti mosquito biting preferences and host shifts. Training and testing to scents of humans and other host species showed that mosquitoes can aversively learn the scent of specific humans and single odorants and learn to avoid the scent of rats (but not chickens). Using pharmacological interventions, RNAi, and CRISPR gene editing, we found that modification of the dopamine-1 receptor suppressed their learning abilities. We further show through combined electrophysiological and behavioral recordings from tethered flying mosquitoes that these odors evoke changes in both behavior and antennal lobe (AL) neuronal responses and that dopamine strongly modulates odor-evoked responses in AL neurons. Not only do these results provide direct experimental evidence that olfactory learning in mosquitoes can play an epidemiological role, but collectively, they also provide neuroanatomical and functional demonstration of the role of dopamine in mediating this learning-induced plasticity, for the first time in a disease vector insect. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Physical characteristics and reproductive performance in Aedes (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    H. Dieng

    2016-12-01

    Full Text Available Body size is a physical factor of crucial importance underlying important traits of the reproductive dynamics of both sexes in mosquitoes. Most studies on the influence of body size in mating success of dengue vectors addressed sperm transfer to females and did not consider egg production, a prerequisite for population maintenance; male body size impact on reproduction has attracted little research interest with respect to sterile insect technique. In experiments involving differently sized adults, we examined whether the body size of the mates is a source of variation in reproductive outcome in Aedes aegypti. In the absence of male partners, large females (LF showed better fecundity than small females (SF. In intraclass mating trials, egg production was much greater in largesized than smallsized pairs. There were comparable fecundities in large females mated with small males and large pairs. [SF•SM] and [SF•LM] pairs showed equivalent fecundity. Nonmating did not result in the production of viable eggs by either small or large females. We also observed that eggs produced by largesized females mated with small males had better hatching success than those from either small or large pairs. Mating between small females and large males resulted in poor egg viability.

  1. Late Devonian spermatophyte diversity and paleoecology at Red Hill, north-central Pennsylvania, USA

    Energy Technology Data Exchange (ETDEWEB)

    Cressler, Walter L. III. [Francis Harvey Green Library, 29 West Rosedale Avenue, West Chester University, West Chester, PA, 19383 (United States); Prestianni, Cyrille [Universite de Liege, Boulevard du Rectorat B18, Liege 4000 (Belgium); LePage, Ben A. [The Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA, 19103 and PECO Energy Company, 2301 Market Avenue, S9-1, Philadelphia, PA 19103 (United States)

    2010-08-01

    Early spermatophytes have been discovered at Red Hill, a Late Devonian (Famennian) fossil locality in north-central Pennsylvania, USA. The Red Hill locality contains an Archaeopteris-dominated flora within an outcrop of the Duncannon Member of the Catskill Formation. Palynological analyses of the plant fossil-bearing horizons within the Red Hill outcrop indicate deposition within the VCo palynozone. This is the earliest time horizon known to contain evidence for spermatophytes, and is contemporaneous with well-known spermatophyte-bearing deposits in West Virginia and Belgium. Some of the spermatophyte material from Red Hill compares well with Aglosperma sp., previously known as isolated ovules from the latest Devonian of South Wales and England, thus extending its geographic and stratigraphic range. Red Hill specimens of Aglosperma sp. occur both as isolated ovules and attached to dichotomously forking axes. Additional spermatophyte cupules discovered at Red Hill are morphologically similar to those of the previously described Late Devonian spermatophytes Elkinsia Rothwell, Scheckler, et Gillespie, Moresnetia Stockmans, and Xenotheca Arber et Goode. Some of the Red Hill cupule complexes are distinct from the aforementioned taxa in consisting of slender dichotomously forking axes terminating in paired cupules with highly fused and symmetric cupule quadrant lobes. The distinctive nature of these Red Hill specimens warrants the creation of Duodimidia pfefferkornii Cressler, Prestianni, et LePage gen. et sp. nov. Plant fossil remains with sphenopteroid foliage are also present at Red Hill, possibly attributable to the spermatophytes. Previous systematic sampling of the rich plant-fossil bearing layer at Red Hill and analysis of its floristic diversity and abundance as well as the presence and absence of charcoal suggests a pattern of floral turnover from a local-scale Rhacophyton-dominated community to spermatophyte colonization following disturbance by wildfires

  2. DOE turns down all bids for Elk Hills crude

    International Nuclear Information System (INIS)

    Anon.

    1992-01-01

    This paper reports that the U.S. Department of Energy has rejected all bids submitted in the Mar. 5 semiannual sale of crude oil from Elk Hills Naval Petroleum Reserve (NPR-1) in California. DOE the all 19 bids for the 53,740 b/d of crude were too low. The bids ranged from $11.71 to $14.06/bbl, with the top bids for the highest quality Stevens zone crude averaging $13.25/bbl. California oil companies the they bid what the market would bear, explaining a surplus of Alaskan crude on the West Coast has driven down the price of local crudes, notably heavy crudes. DOE will extend the current oil purchase contracts through April while it issues a new request for bids. It planned to issue the solicitation Mar. 23 and receive bids Apr. 15

  3. Column Experiments to Interpret Weathering in Columbia Hills

    Science.gov (United States)

    Hausrath, E. M.; Morris, R.V.; Ming, D.W.; Golden, D.C.; Galindo, C.; Sutter, B.

    2009-01-01

    Phosphate mobility has been postulated as an indicator of early aqueous activity on Mars. In addition, rock surfaces analyzed by the Mars Exploration Rover Spirit are consistent with the loss of a phosphate- containing mineral To interpret phosphate alteration behavior on Mars, we performed column dissolution experiments leaching the primary phases Durango fluorapatite, San Carlos olivine, and basalt glass (Stapafjell Volcano, courtesy of S. Gislason, University of Iceland) [3,4]) with acidic solutions. These phases were chosen to represent quickly dissolving phases likely present in Columbia Hills. Column dissolution experiments are closer to natural dissolution conditions than batch experiments, although they can be difficult to interpret. Acidic solutions were used because the leached layers on the surfaces of these rocks have been interpreted as resulting from acid solutions [5].

  4. Hills and valleys: Understanding the under-eye

    Directory of Open Access Journals (Sweden)

    Milind N Naik

    2016-01-01

    Full Text Available Soft tissue deflation and descent have long been implicated in the pathogenesis of facial aging. In the periorbital area, the upper orbital region is thought to change by descent of the eyebrow, as well as deflation of brow fat. While the understanding of the aging changes in the upper eyelid region are relatively simple, the lower eyelid poses a myriad of aging changes, each demanding a specific management plan. These can be best described in terms of elevations, or 'Hills' and hollows, or 'Valleys'. This article simplifies the understanding of the lower eyelid in the light of anatomical knowledge, and available literature. It forms a basis of easy diagnosis and treatment of the soft tissue changes in the lower eyelid and malar region.

  5. A new Lower Triassic ichthyopterygian assemblage from Fossil Hill, Nevada

    Directory of Open Access Journals (Sweden)

    Neil P. Kelley

    2016-01-01

    Full Text Available We report a new ichthyopterygian assemblage from Lower Triassic horizons of the Prida Formation at Fossil Hill in central Nevada. Although fragmentary, the specimens collected so far document a diverse fauna. One partial jaw exhibits isodont dentition with blunt tipped, mesiodistally compressed crowns and striated enamel. These features are shared with the Early Triassic genus Utatsusaurus known from coeval deposits in Japan and British Columbia. An additional specimen exhibits a different dentition characterized by relatively small, rounded posterior teeth resembling other Early Triassic ichthyopterygians, particularly Grippia. This Nevada assemblage marks a southward latitudinal extension for Early Triassic ichthyopterygians along the eastern margin of Panthalassa and indicates repeated trans-hemispheric dispersal events in Early Triassic ichthyopterygians.

  6. Analysis of ancient pottery from the palatine hill in Rome

    International Nuclear Information System (INIS)

    De Sena, E.; Landsberger, S.; Wisseman, S.

    1995-01-01

    A program of compositional analysis using neutron activation has been performed on samples of Roman fine ware from the Palatine East excavations in Rome at the University of Illinois' TRIGA reactor. These experiments are ultimately intended to assist the authors in advancing the understanding of the organization of pottery production and distribution in central Italy during the late Roman imperial period (4th-5th c. AD). The objectives of this paper are to present an archaeological background of two regionally-produced fine wares, to discuss the methods of sampling, irradiation and data analyses, and to demonstrate the preliminary results of our investigation, which included the analyses of Plio-Pleistocene clays from the Janiculum Hill in Rome. (author). 5 refs., 2 figs., 6 tabs

  7. Draft tube flow phenomena across the bulb turbine hill chart

    International Nuclear Information System (INIS)

    Duquesne, P; Fraser, R; Maciel, Y; Aeschlimann, V; Deschênes, C

    2014-01-01

    In the framework of the BulbT project launched by the Consortium on Hydraulic Machines and the LAMH (Hydraulic Machine Laboratory of Laval University) in 2011, an intensive campaign to identify flow phenomena in the draft tube of a model bulb turbine has been done. A special focus was put on the draft tube component since it has a particular importance for recuperation in low head turbines. Particular operating points were chosen to analyse flow phenomena in this component. For each of these operating points, power, efficiency and pressure were measured following the IEC 60193 standard. Visualizations, unsteady wall pressure and efficiency measurements were performed in this component. The unsteady wall pressure was monitored at seven locations in the draft tube. The frequency content of each pressure signal was analyzed in order to characterize the flow phenomena across the efficiency hill chart. Visualizations were recorded with a high speed camera using tufts and cavitation bubbles as markers. The predominant detected phenomena were mapped and categorized in relation to the efficiency hill charts obtained for three runner blade openings. At partial load, the vortex rope was detected and characterized. An inflection in the partial load efficiency curves was found to be related to complex vortex rope instabilities. For overload conditions, the efficiency curves present a sharp drop after the best efficiency point, corresponding to an inflection on the power curves. This break off is more severe towards the highest blade openings. It is correlated to a flow separation at the wall of the draft tube. Also, due to the separation occurring in these conditions, a hysteresis effect was observed on the efficiency curves

  8. Surface and Flow Field Measurements on the FAITH Hill Model

    Science.gov (United States)

    Bell, James H.; Heineck, James T.; Zilliac, Gregory; Mehta, Rabindra D.; Long, Kurtis R.

    2012-01-01

    A series of experimental tests, using both qualitative and quantitative techniques, were conducted to characterize both surface and off-surface flow characteristics of an axisymmetric, modified-cosine-shaped, wall-mounted hill named "FAITH" (Fundamental Aero Investigates The Hill). Two separate models were employed: a 6" high, 18" base diameter machined aluminum model that was used for wind tunnel tests and a smaller scale (2" high, 6" base diameter) sintered nylon version that was used in the water channel facility. Wind tunnel and water channel tests were conducted at mean test section speeds of 165 fps (Reynolds Number based on height = 500,000) and 0.1 fps (Reynolds Number of 1000), respectively. The ratio of model height to boundary later height was approximately 3 for both tests. Qualitative techniques that were employed to characterize the complex flow included surface oil flow visualization for the wind tunnel tests, and dye injection for the water channel tests. Quantitative techniques that were employed to characterize the flow included Cobra Probe to determine point-wise steady and unsteady 3D velocities, Particle Image Velocimetry (PIV) to determine 3D velocities and turbulence statistics along specified planes, Pressure Sensitive Paint (PSP) to determine mean surface pressures, and Fringe Imaging Skin Friction (FISF) to determine surface skin friction (magnitude and direction). This initial report summarizes the experimental set-up, techniques used, data acquired and describes some details of the dataset that is being constructed for use by other researchers, especially the CFD community. Subsequent reports will discuss the data and their interpretation in more detail

  9. Aedes albopictus mosquito: the main vector of the 2007 Chikungunya outbreak in Gabon.

    Directory of Open Access Journals (Sweden)

    Frédéric Pagès

    Full Text Available The primary vector at the origin of the 2007 outbreak in Libreville, Gabon is identified as Aedes albopictus, trapped around the nearby French military camp. The Chikungunya virus was isolated from mosquitoes and found to be identical to the A226V circulating human strain. This is the first field study showing the role of the recently arrived species Aedes albopictus in Chikungunya virus transmission in Central Africa, and it demonstrates this species' role in modifying the epidemiological presentation of Chikungunya in Gabon.

  10. A highly stable blood meal alternative for rearing Aedes and Anopheles mosquitoes.

    Directory of Open Access Journals (Sweden)

    Ted Baughman

    2017-12-01

    Full Text Available We investigated alternatives to whole blood for blood feeding of mosquitoes with a focus on improved stability and compatibility with mass rearing programs. In contrast to whole blood, an artificial blood diet of ATP-supplemented plasma was effective in maintaining mosquito populations and was compatible with storage for extended periods refrigerated, frozen, and as a lyophilized powder. The plasma ATP diet supported rearing of both Anopheles and Aedes mosquitoes. It was also effective in rearing Wolbachia-infected Aedes mosquitoes, suggesting compatibility with vector control efforts.

  11. Theory of synergistic effects: Hill-type response surfaces as 'null-interaction' models for mixtures.

    Science.gov (United States)

    Schindler, Michael

    2017-08-02

    The classification of effects caused by mixtures of agents as synergistic, antagonistic or additive depends critically on the reference model of 'null interaction'. Two main approaches are currently in use, the Additive Dose (ADM) or concentration addition (CA) and the Multiplicative Survival (MSM) or independent action (IA) models. We compare several response surface models to a newly developed Hill response surface, obtained by solving a logistic partial differential equation (PDE). Assuming that a mixture of chemicals with individual Hill-type dose-response curves can be described by an n-dimensional logistic function, Hill's differential equation for pure agents is replaced by a PDE for mixtures whose solution provides Hill surfaces as 'null-interaction' models and relies neither on Bliss independence or Loewe additivity nor uses Chou's unified general theory. An n-dimensional logistic PDE decribing the Hill-type response of n-component mixtures is solved. Appropriate boundary conditions ensure the correct asymptotic behaviour. Mathematica 11 (Wolfram, Mathematica Version 11.0, 2016) is used for the mathematics and graphics presented in this article. The Hill response surface ansatz can be applied to mixtures of compounds with arbitrary Hill parameters. Restrictions which are required when deriving analytical expressions for response surfaces from other principles, are unnecessary. Many approaches based on Loewe additivity turn out be special cases of the Hill approach whose increased flexibility permits a better description of 'null-effect' responses. Missing sham-compliance of Bliss IA, known as Colby's model in agrochemistry, leads to incompatibility with the Hill surface ansatz. Examples of binary and ternary mixtures illustrate the differences between the approaches. For Hill-slopes close to one and doses below the half-maximum effect doses MSM (Colby, Bliss, Finney, Abbott) predicts synergistic effects where the Hill model indicates 'null

  12. Vapor toxicity of five volatile pyrethroids against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles quadrimaculatus (Diptera: Culicidae).

    Science.gov (United States)

    Bibbs, Christopher S; Tsikolia, Maia; Bloomquist, Jeffrey R; Bernier, Ulrich R; Xue, Rui-De; Kaufman, Phillip E

    2018-05-25

    Mosquito mortality has been documented in numerous studies of spatial repellents but the concentration-dependent toxicity of spatial repellent vapors has not been documented. To address this issue, prallethrin, flumethrin, metofluthrin, transfluthrin, and meperfluthrin were selected for comparative study against Aedes albopictus (Skuse), Ae. aegypti (L.), Culex quinquefasciatus Say, and Anopheles quadrimaculatus Say. Mosquito were exposed to vapors of each chemical for 2h, 4h, and 24h with mortality recorded at each time point. A second experiment involved exposing mosquitoes to vapors for 2h, then transferring them to untreated holding containers and held for 24h. For these mosquitoes, readings were only taken after 24h to allow for metabolic detoxification and recovery. The LC 50 and LC 90 data indicated that transfluthrin and meperfluthrin had the greatest toxicity across all species, followed by metofluthrin, prallethrin, and flumethrin. Our findings, through the direct comparison of these compounds, suggest that transfluthrin, meperfluthrin, and metofluthrin be considered for further development. The vapor toxicity for the aforementioned compounds significantly exceeds prallethrin, which is currently market available as an adulticidal active ingredient in public health pest control. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  13. Voz sobre frame relay

    OpenAIRE

    D´Elia, Gabriel Anibal

    2000-01-01

    Esta tesis trata el tema de VOFR, desde la digitalización de la voz hasta su transmisión a través de dicha red, así también como la comparación con otros medios de transporte como VOIP. Dada las características del protocolo frame relay y su disponibilidad se eligió como el medio más apropiado para la transmisión de voz y datos en forma integrada sobre una misma red. El trabajo comienza con una breve explicación de la voz, su digitalización y forma actual de transmisión a través de una red di...

  14. Larvicidal activity of the water extract of Moringa oleifera seeds against Aedes aegypti and its toxicity upon laboratory animals

    Directory of Open Access Journals (Sweden)

    Paulo M.P. Ferreira

    2009-06-01

    Full Text Available In this work, biological effects of the water extract of Moringa oleifera seeds (WEMOS were assessed on eggs and 3rd instar larvae of Aedes aegypti and on its toxicity upon laboratory animals (Daphnia magna, mice and rats. Crude WEMOS showed a LC50 value of 1260µg/mL, causing 99.2 ± 2.9% larvae mortality within 24 h at 5200µg/mL, though this larvicidal activity has been lost completely at 80ºC/10 min. WEMOS did not demonstrate capacity to prevent egg hatching. After extensive dialyses of the crude WEMOS into watersoluble dialyzable (DF and nondyalizable (NDF fractions, only DF maintained its efficacy to kill larvae. Acute toxicity evaluations on daphnids (EC50 of 188.7µg/mL and mice (LD50 of 446.5 mg/kg body weight pointed out to low toxicity. Despite the thymus hypertrophy, WEMOS revealed to be harmless in orally and subacutelytreated rats. In conclusion, WEMOS has thermostable bioactive compounds against Ae. aegypti larvae with apparent molecular mass lower than 12 kDa and moderately toxic potential.Neste trabalho, o extrato aquoso das sementes de Moringaoleifera (EASMO foi avaliado quanto aos seus efeitos biológicos sobre ovos e larvas de Aedes aegypti no 3ºestágio de desenvolvimento e sua toxicidade sobre animais de laboratório(Daphnia magna, camundongos e ratos. O EASMO bruto revelou uma CL50 de 1.260 µg/mL, causando 99, 2 ± 2, 9% de mortalidade em 24 h na concentração de 5.200 µg/mL, embora o mesmo não tenha sido capaz de impedir a eclosão dos ovos. A atividade larvicida extinguiu-se após aquecimento do extrato a 80ºC/10 min. Diálises sucessivas do EASMO bruto resultaram em duas frações solúveis em água (Fração dializável, FD; Fração nãodializável, FND, dentre as quais apenas a FD mostrou ação larvicida. Testes de toxicidade aguda realizadosem dáfnias (CE50 de 188, 7 µg/mL e camundongos (DL50 de446,5 mg/kg de peso corpóreo evidenciaram baixa toxicidade. Apesar da hipertrofia tímica, o EASMO mostrou ser

  15. Conditioning exercises in ski jumping: biomechanical relationship of squat jumps, imitation jumps, and hill jumps.

    Science.gov (United States)

    Lorenzetti, Silvio; Ammann, Fabian; Windmüller, Sabrina; Häberle, Ramona; Müller, Sören; Gross, Micah; Plüss, Michael; Plüss, Stefan; Schödler, Berni; Hübner, Klaus

    2017-11-22

    As hill jumps are very time-consuming, ski jumping athletes often perform various imitation jumps during training. The performed jumps should be similar to hill jumps, but a direct comparison of the kinetic and kinematic parameters has not been performed yet. Therefore, this study aimed to correlate 11 common parameters during hill jumps (Oberstdorf Germany), squat jumps (wearing indoor shoes), and various imitation jumps (rolling 4°, rolling flat, static; jumping equipment or indoor shoes) on a custom-built instrumented vehicle with a catch by the coach. During the performed jumps, force and video data of the take-off of 10 athletes were measured. The imitation and squat jumps were then ranked. The main difference between the hill jumps and the imitation and squat jumps is the higher maximal force loading rate during the hill jumps. Imitation jumps performed on a rolling platform, on flat ground were the most similar to hill jumps in terms of the force-time, and leg joint kinematic properties. Thus, non-hill jumps with a technical focus should be performed from a rolling platform with a flat inrun with normal indoor shoes or jumping equipment, and high normal force loading rates should be the main focus of imitation training.

  16. Field evaluation of the Off! Clip-on Mosquito Repellent (metofluthrin) against Aedes albopictus and Aedes taeniorhynchus (Diptera: Culicidae) in northeastern Florida.

    Science.gov (United States)

    Xue, Rui-De; Qualls, Whitney A; Smith, Michael L; Gaines, Marcia K; Weaver, James H; Debboun, Mustapha

    2012-05-01

    Repellent efficacy of the Off! Clip-on Mosquito Repellent device (S. C. Johnson and Son, Inc., Racine, WI) containing Metofluthrin was evaluated on six human volunteers against the container-breeding mosquito Aedes albopictus (Skuse) and the salt marsh mosquito Aedes taeniorhynchus (Wiedemann) at two field locations in northeastern Florida. The device repelled mosquitoes by releasing a vaporized form of the pyrethroid insecticide metofluthrin ([AI] 31.2%) and provided 70% protection from Ae. albopictus bites for > 3 h. For the second field trial, a repellent device that was used in the first trial was tested after being open for >1 wk. This device provided 79% protection from Ae. taeniorhynchus bites for 3 h. Our field results showed that the repellent device was 70 and 79% effective at repelling Ae. albopictus and Ae. taeniorhynchus from human test subjects in both field locations in northeastern Florida.

  17. Temporal distribution and insecticide resistance profile of two major arbovirus vectors Aedes aegypti and Aedes albopictus in Yaoundé, the capital city of Cameroon.

    Science.gov (United States)

    Kamgang, Basile; Yougang, Aurelie P; Tchoupo, Micareme; Riveron, Jacob M; Wondji, Charles

    2017-10-10

    Aedes aegypti and Ae. albopictus are the major epidemic vectors of several arbovirus diseases such as yellow fever, dengue, Zika and chikungunya worldwide. Both Aedes vectors are presents in Cameroon; however, knowledge on the dynamic of the distribution of these species across cities and their resistance profile to insecticide are limited. Here, we assessed the current distribution of Ae. aegypti and Ae. albopictus in Yaoundé, the Capital City, established the resistance profile to insecticides and explored the resistance mechanisms involved. Immature stages of Aedes were sampled in several breeding sites in December 2015 (dry season) and June 2016 (rainy season) in three central neighborhoods and four peripheral neighborhoods and reared to adult stage. The G0 adults were used for molecular identification and genotyping of F1534C mutation in Ae. aegypti. Bioassays and piperonyl butoxide (PBO) assays were carried out according to WHO guidelines. Analysis revealed that both species Ae. aegypti and Ae. albopictus are present in all prospected sites in Yaounde. However, in the dry season Ae. aegypti is most abundant in neighborhoods located in downtown. In contrast, Ae. albopictus was found most prevalent in suburbs whatever the season and in downtown during the rainy season. Bioassay analysis showed that both Ae. aegypti and Ae. albopictus, are resistant to 0.05% deltamethrin, 0.1% bendiocarb and 4% dichlorodiphenyltrichloroethane (DDT). A decreased of susceptibility to 0.75% permethrin and a full susceptibility to malathion 5% was observed. The mortality rate was increased after pre-exposure to synergist PBO. None of Ae. aegypti assayed revealed the presence of F1534C mutation. These findings are useful to planning vector control programme against arbovirus vectors in Cameroon and can be used as baseline in Africa where data on Aedes resistance is very scarce to plan further works.

  18. Maternal invasion history of Aedes aegypti and Aedes albopictus into the Isthmus of Panama: Implications for the control of emergent viral disease agents

    Science.gov (United States)

    Eskildsen, Gilberto A.; Rovira, Jose R.; Smith, Octavio; Miller, Matthew J.; Bennett, Kelly L.; McMillan, W. Owen

    2018-01-01

    Despite an increase in dengue outbreaks and the arrival of chikungunya and Zika disease in Panama, studies on the demographic history of the invasive Aedes mosquitoes that are the principle vectors of these diseases are still lacking in this region. Here, we assess the genetic diversity of these mosquitoes in order to decipher their invasion histories into the Isthmus of Panama. DNA sequences from the mitochondrial cytochrome C oxidase I gene obtained from 30 localities in 10 provinces confirmed the presence of more than one mitochondrial haplogroup (i.e., maternal lineage) in each species. The invasion of Aedes albopictus was likely from temperate European countries, as the most frequent and widespread haplogroup in Panama harbored variants that are uncommon elsewhere in the Americas. Two infrequent and geographically restricted Ae. albopictus haplotypes appear to have subsequently invaded Panama from neighboring Costa Rica and the USA, respectively. In addition, we recovered two deeply divergent mitochondrial clades in Panamanian Aedes aegypti. The geographic origins of these clades is unknown, given that divergence in the mitochondrial genome is probably due to ancient population processes within the native range of Ae. aegypti, rather than due to its global expansion out of Africa. However, Panamanian Ae. aegypti mitochondrial sequences within the first clade were closely related to others from Colombia, Bolivia, Brazil, Mexico and the USA, suggesting two separate invasions from Western Hemisphere source populations. The pattern of increased genetic diversity in Aedes mosquitoes in Panama is likely facilitated by the numerous land and water inter-connections across the country, which allows them to enter via sea- and land-transportation from Europe, North, Central and South America. Our results here should be considered in disease mitigation programs if emergent arboviruses are to be effectively diminished in Panama through vector suppression. PMID:29579112

  19. Principales criaderos para Aedes aegypti y culícidos asociados, Argentina Main breeding-containers for Aedes aegypti and associated culicids, Argentina

    Directory of Open Access Journals (Sweden)

    Marina Stein

    2002-10-01

    Full Text Available Se identificaron criaderos de Aedes (Stegomyia aegypti en dos ciudades de la provincia del Chaco (Noreste de Argentina: Presidencia Roque Saenz Peña y Machagai. Los recipientes encontrados en los patios de las viviendas capaces de colectar agua se clasificaron según tipo y tamaño, se contaron y examinaron. Aedes aegypti y Culex quinquefasciatus fueron las especies mas abundantes, encontrándose además Cx. maxi, Cx. saltanensis y Ochlerotatus scapularis. Los neumáticos y cajas de baterías para autos fueron los recipientes que más aportaron a la producción de formas inmaduras de los culícidos colectados. Las lluvias fueron un factor importante para la proliferacion de Ae. aegypti, así como también el habito comun en la población de guardar recipientes en sus casas que permitan el desarrollo de estos culícidos.Breeding containers for Aedes (Stegomyia aegypti were identified in two cities of Chaco Province (northeast Argentina: Presidencia Roque Saenz Peña and Machagai. All water-retaining recipients found in house backyards capable to retain water were classified according to their type and size, counted and checked. Aedes aegypti and Culex quinquefasciatus were the most frequently collected species, being also found Cx. maxi, Cx. saltanensis and Ochlerotatus scapularis. Tires and car batteries represented the most important type of container where immature forms of culicids could be found. Rain was an important factor for Ae. aegypti proliferation, as well as the widespread habit of the population of keeping useless containers at home, which allows the development of culicids.

  20. Effects of larval crowding on development time, survival and weight at metamorphosis in Aedes aegypti (Diptera: Culicidae

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    Arnaldo MACIÁ

    2009-01-01

    Full Text Available Los efectos del hacinamiento larval sobre el tiempo de desarrollo, la supervivencia y el peso en la metamorfosis fueron estudiados en el mosquito del dengue, Aedes aegypti L., en el laboratorio. Se criaron cohortes de larvas en 7 densidades (4, 8, 16, 32, 64, 128 y 256 larvas/ recipiente de 175 ml mientras se mantuvo constante el volumen de agua y la calidad y cantidad de alimento, bajo fotoperíodo y temperatura controlados. Se usaron detritos naturales, principalmente hojas, obtenidos de contenedores colonizados naturalmente por A. aegypti como fuente de nutrientes para las larvas. En cada densidad se registraron el tiempo de desarrollo, la mortalidad, el peso en la metamorfosis y la biomasa total. El tiempo de desarrollo varió entre 4 y 23 días en los machos, y 5 a 24 días en hembras; fue más prolongado a la densidad de 64 (en las hembras y 128 (en los machos larvas por recipiente. En densidades altas la proporción de sexos favoreció los machos. Hubo un incremento en la mortalidad en densidades iguales o mayores que 0,4 larvas/ ml (0,32 larvas/cm2. Se detectó una relación inversa entre la densidad larval y el peso de las pupas. La biomasa por individuo alcanzó un valor asintótico de aproximadamente 1 mg/individuo en una densidad de 128 individuos/ recipiente (0,64 larvas/cm2. Las poblaciones de A. aegypti, cercanas a su extremo sur de distribución, serían sensibles al hacinamiento en pequeños contenedores de agua.

  1. Resistance of Aedes aegypti to temephos and adaptive disadvantages

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    Morgana Michele Cavalcanti de Souza Leal Diniz

    2014-10-01

    Full Text Available OBJECTIVE To evaluate the resistance of Aedes aegypti to temephos Fersol 1G (temephos 1% w/w associated with the adaptive disadvantage of insect populations in the absence of selection pressure. METHODS A diagnostic dose of 0.28 mg a.i./L and doses between 0.28 mg a.i./L and 1.40 mg a.i./L were used. Vector populations collected between 2007 and 2008 in the city of Campina Grande, state of Paraíba, were evaluated. To evaluate competition in the absence of selection pressure, insect populations with initial frequencies of 20.0%, 40.0%, 60.0%, and 80.0% resistant individuals were produced and subjected to the diagnostic dose for two months. Evaluation of the development of aquatic and adult stages allowed comparison of the life cycles in susceptible and resistant populations and construction of fertility life tables. RESULTS No mortality was observed in Ae. aegypti populations subjected to the diagnostic dose of 0.28 mg a.i./L. The decreased mortality observed in populations containing 20.0%, 40.0%, 60.0%, and 80.0% resistant insects indicates that temephos resistance is unstable in the absence of selection pressure. A comparison of the life cycles indicated differences in the duration and viability of the larval phase, but no differences were observed in embryo development, sex ratio, adult longevity, and number of eggs per female. CONCLUSIONS The fertility life table results indicated that some populations had reproductive disadvantages compared with the susceptible population in the absence of selection pressure, indicating the presence of a fitness cost in populations resistant to temephos.

  2. Multiple introductions of the dengue vector, Aedes aegypti, into California.

    Science.gov (United States)

    Pless, Evlyn; Gloria-Soria, Andrea; Evans, Benjamin R; Kramer, Vicki; Bolling, Bethany G; Tabachnick, Walter J; Powell, Jeffrey R

    2017-08-01

    The yellow fever mosquito Aedes aegypti inhabits much of the tropical and subtropical world and is a primary vector of dengue, Zika, and chikungunya viruses. Breeding populations of A. aegypti were first reported in California (CA) in 2013. Initial genetic analyses using 12 microsatellites on collections from Northern CA in 2013 indicated the South Central US region as the likely source of the introduction. We expanded genetic analyses of CA A. aegypti by: (a) examining additional Northern CA samples and including samples from Southern CA, (b) including more southern US populations for comparison, and (c) genotyping a subset of samples at 15,698 SNPs. Major results are: (1) Northern and Southern CA populations are distinct. (2) Northern populations are more genetically diverse than Southern CA populations. (3) Northern and Southern CA groups were likely founded by two independent introductions which came from the South Central US and Southwest US/northern Mexico regions respectively. (4) Our genetic data suggest that the founding events giving rise to the Northern CA and Southern CA populations likely occurred before the populations were first recognized in 2013 and 2014, respectively. (5) A Northern CA population analyzed at multiple time-points (two years apart) is genetically stable, consistent with permanent in situ breeding. These results expand previous work on the origin of California A. aegypti with the novel finding that this species entered California on multiple occasions, likely some years before its initial detection. This work has implications for mosquito surveillance and vector control activities not only in California but also in other regions where the distribution of this invasive mosquito is expanding.

  3. Is Switzerland suitable for the invasion of Aedes albopictus [corrected]?

    Science.gov (United States)

    Neteler, Markus; Metz, Markus; Rocchini, Duccio; Rizzoli, Annapaola; Flacio, Eleonora; Engeler, Luca; Guidi, Valeria; Lüthy, Peter; Tonolla, Mauro

    2013-01-01

    Over the last 30 years, the Asian tiger mosquito, Aedes albopictus, has rapidly spread around the world. The European distribution comprises the Mediterranean basin with a first appearance in Switzerland in 2003. Early identification of the most suitable areas in Switzerland allowing progressive invasion by this species is considered crucial to suggest adequate surveillance and control plans. We identified the most suitable areas for invasion and establishment of Ae. albopictus in Switzerland. The potential distribution areas linked to the current climatic suitability were assessed using remotely sensed land surface temperature data recorded by the MODIS satellite sensors. Suitable areas for adult survival and overwintering of diapausing eggs were also identified for future climatic conditions, considering two different climate change scenarios (A1B, A2) for the periods 2020-2049 and 2045-2074. At present, the areas around Lake Geneva in western Switzerland provide suitable climatic conditions for Ae. albopictus. In northern Switzerland, parts of the Rhine valley, around Lake Constance, as well as the surroundings of Lake Neuchâtel, appear to be suitable for the survival at least of adult Ae. albopictus. However, these areas are characterized by winters currently being too cold for survival and development of diapausing eggs. In southern Switzerland, Ae. albopictus is already well-established, especially in the Canton of Ticino. For the years 2020-2049, the predicted possible spread of the tiger mosquito does not differ significantly from its potential current distribution. However, important expansions are obtained if the period is extended to the years 2045-2074, when Ae. albopictus may invade large new areas. Several parts of Switzerland provide suitable climatic conditions for invasion and establishment of Ae. albopictus. The current distribution and rapid spread in other European countries suggest that the tiger mosquito will colonize new areas in Switzerland

  4. Is Switzerland Suitable for the Invasion of Aedes albopictus?

    Science.gov (United States)

    Neteler, Markus; Metz, Markus; Rocchini, Duccio; Rizzoli, Annapaola; Flacio, Eleonora; Engeler, Luca; Guidi, Valeria; Lüthy, Peter; Tonolla, Mauro

    2013-01-01

    Background Over the last 30 years, the Asian tiger mosquito, Aedes albopictus, has rapidly spread around the world. The European distribution comprises the Mediterranean basin with a first appearance in Switzerland in 2003. Early identification of the most suitable areas in Switzerland allowing progressive invasion by this species is considered crucial to suggest adequate surveillance and control plans. Methodology/Principal Findings We identified the most suitable areas for invasion and establishment of Ae. albopictus in Switzerland. The potential distribution areas linked to the current climatic suitability were assessed using remotely sensed land surface temperature data recorded by the MODIS satellite sensors. Suitable areas for adult survival and overwintering of diapausing eggs were also identified for future climatic conditions, considering two different climate change scenarios (A1B, A2) for the periods 2020–2049 and 2045–2074. At present, the areas around Lake Geneva in western Switzerland provide suitable climatic conditions for Ae. albopictus. In northern Switzerland, parts of the Rhine valley, around Lake Constance, as well as the surroundings of Lake Neuchâtel, appear to be suitable for the survival at least of adult Ae. albopictus. However, these areas are characterized by winters currently being too cold for survival and development of diapausing eggs. In southern Switzerland, Ae. albopictus is already well-established, especially in the Canton of Ticino. For the years 2020–2049, the predicted possible spread of the tiger mosquito does not differ significantly from its potential current distribution. However, important expansions are obtained if the period is extended to the years 2045–2074, when Ae. albopictus may invade large new areas. Conclusions/Significance Several parts of Switzerland provide suitable climatic conditions for invasion and establishment of Ae. albopictus. The current distribution and rapid spread in other European

  5. Evolutionary enhancement of Zika virus infectivity in Aedes aegypti mosquitoes.

    Science.gov (United States)

    Liu, Yang; Liu, Jianying; Du, Senyan; Shan, Chao; Nie, Kaixiao; Zhang, Rudian; Li, Xiao-Feng; Zhang, Renli; Wang, Tao; Qin, Cheng-Feng; Wang, Penghua; Shi, Pei-Yong; Cheng, Gong

    2017-05-25

    Zika virus (ZIKV) remained obscure until the recent explosive outbreaks in French Polynesia (2013-2014) and South America (2015-2016). Phylogenetic studies have shown that ZIKV has evolved into African and Asian lineages. The Asian lineage of ZIKV was responsible for the recent epidemics in the Americas. However, the underlying mechanisms through which ZIKV rapidly and explosively spread from Asia to the Americas are unclear. Non-structural protein 1 (NS1) facilitates flavivirus acquisition by mosquitoes from an infected mammalian host and subsequently enhances viral prevalence in mosquitoes. Here we show that NS1 antigenaemia determines ZIKV infectivity in its mosquito vector Aedes aegypti, which acquires ZIKV via a blood meal. Clinical isolates from the most recent outbreak in the Americas were much more infectious in mosquitoes than the FSS13025 strain, which was isolated in Cambodia in 2010. Further analyses showed that these epidemic strains have higher NS1 antigenaemia than the FSS13025 strain because of an alanine-to-valine amino acid substitution at residue 188 in NS1. ZIKV infectivity was enhanced by this amino acid substitution in the ZIKV FSS13025 strain in mosquitoes that acquired ZIKV from a viraemic C57BL/6 mouse deficient in type I and II interferon (IFN) receptors (AG6 mouse). Our results reveal that ZIKV evolved to acquire a spontaneous mutation in its NS1 protein, resulting in increased NS1 antigenaemia. Enhancement of NS1 antigenaemia in infected hosts promotes ZIKV infectivity and prevalence in mosquitoes, which could have facilitated transmission during recent ZIKV epidemics.

  6. Wolbachia-associated bacterial protection in the mosquito Aedes aegypti.

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    Yixin H Ye

    Full Text Available BACKGROUND: Wolbachia infections confer protection for their insect hosts against a range of pathogens including bacteria, viruses, nematodes and the malaria parasite. A single mechanism that might explain this broad-based pathogen protection is immune priming, in which the presence of the symbiont upregulates the basal immune response, preparing the insect to defend against subsequent pathogen infection. A study that compared natural Wolbachia infections in Drosophila melanogaster with the mosquito vector Aedes aegypti artificially transinfected with the same strains has suggested that innate immune priming may only occur in recent host-Wolbachia associations. This same study also revealed that while immune priming may play a role in viral protection it cannot explain the entirety of the effect. METHODOLOGY/FINDINGS: Here we assess whether the level of innate immune priming induced by different Wolbachia strains in A. aegypti is correlated with the degree of protection conferred against bacterial pathogens. We show that Wolbachia strains wMel and wMelPop, currently being tested for field release for dengue biocontrol, differ in their protective abilities. The wMelPop strain provides stronger, more broad-based protection than wMel, and this is likely explained by both the higher induction of immune gene expression and the strain-specific activation of particular genes. We also show that Wolbachia densities themselves decline during pathogen infection, likely as a result of the immune induction. CONCLUSIONS/SIGNIFICANCE: This work shows a correlation between innate immune priming and bacterial protection phenotypes. The ability of the Toll pathway, melanisation and antimicrobial peptides to enhance viral protection or to provide the basis of malaria protection should be further explored in the context of this two-strain comparison. This work raises the questions of whether Wolbachia may improve the ability of wild mosquitoes to survive pathogen

  7. Factors influencing stakeholders attitudes toward genetically modified aedes mosquito.

    Science.gov (United States)

    Amin, Latifah; Hashim, Hasrizul

    2015-06-01

    Dengue fever is a debilitating and infectious disease that could be life-threatening. It is caused by the dengue virus which affects millions of people in the tropical area. Currently, there is no cure for the disease as there is no vaccine available. Thus, prevention of the vector population using conventional methods is by far the main strategy but has been found ineffective. A genetically modified (GM) mosquito is among the favoured alternatives to curb dengue fever in Malaysia. Past studies have shown that development and diffusion of gene technology products depends heavily upon public acceptance. The purpose of this study is to identify the relevant factors influencing stakeholders' attitudes toward the GM Aedes mosquito and to analyse the relationships between all the factors using the structural equation model. A survey was carried out on 509 respondents from various stakeholder groups in the Klang Valley region of Malaysia. Results of the survey have confirmed that public perception towards complex issues such as gene technology should be seen as a multi-faceted process. The perceived benefit-perceived risk balance is very important in determining the most predominant predictor of attitudes toward a GM mosquito. In this study the stakeholders perceived the benefit of the GM mosquito as outweighing its risk, translating perceived benefit as the most important direct predictor of attitudes toward the GM mosquito. Trust in key players has a direct influence on attitudes toward the GM mosquito while moral concern exhibited an indirect influence through perceived benefits. Other factors such as attitudes toward technology and nature were also indirect predictors of attitudes toward the GM mosquito while religiosity and engagement did not exhibited any significant roles. The research findings serve as a useful database to understand public acceptance and the social construct of public attitudes towards the GM mosquito to combat dengue.

  8. PENGENDALIAN JENTIK Aedes Aegypti MENGGUNAKAN Mesocyclops Aspericomis MELALUI PARTISIPASI MASYARAKAT

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    Umi Widyastuti

    2012-07-01

    Full Text Available Mesocyclops aspericornis was investigated for its effectiveness in controlling Aedes aegypti larvae in a variety of containers e.g metal drum, cistern, clay jars, and other container made of plastic. A study was carried out in Kenteng hamlet, Tegalrejo village, Salatiga Municipality. It was conducted by health-workers (staff of Vector and Reservoir Control Research Unit and Health Center of Tegalrejo and the community, especially the woman's organization namely "family empowering and welfareness ". which participate in releasing M. aspericornis for controlling Ae. aegypti larvae. The community has responsibility to release M. aspericornis in Kenteng RT01 and 02 as the treated area I. Meanwhile, Health-workers have responsibility to release it in Kenteng RT 04, 05, and 07 as the treated area II and Kenteng RT 03 and 06 as the untreated control area (no M. aspericornis released. The aim of the study were: a, to determine the effectiveness of M. aspericornis in decreasing larval populations of Ae. aegypti in the containers, and b. to determine the Knowledge, Attitude, and Practice (KAP of the community, referring to disease, vector and control of Dengue Haemorhagic Fever (DHF. M. aspericornis was effective to decrease larval populations of Ae. aegypti in Kenteng area. The increasing number of Ae. aegypti larvae free containers of 24.29-84,02% and 35.75-92.01% were shown in respectively treated area I and II. The KAP of the community referring to disease, vector and control of DHF increased after the health education conducted. It's concluded that the community of Kenteng hamlet is active in participation to control Ae. aegypti. As a recommend, control of Ae. aegypti larvae using M. aspericornis through community partisipation should be considered due to a good prospect and effectiveness of this agent to control of Ae. aegypti larvae in the laboratory as well as in the field   Key words : Vector control, M. aspericornis, Ae. aegypti, Dengue

  9. Multiple introductions of the dengue vector, Aedes aegypti, into California.

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    Evlyn Pless

    2017-08-01

    Full Text Available The yellow fever mosquito Aedes aegypti inhabits much of the tropical and subtropical world and is a primary vector of dengue, Zika, and chikungunya viruses. Breeding populations of A. aegypti were first reported in California (CA in 2013. Initial genetic analyses using 12 microsatellites on collections from Northern CA in 2013 indicated the South Central US region as the likely source of the introduction. We expanded genetic analyses of CA A. aegypti by: (a examining additional Northern CA samples and including samples from Southern CA, (b including more southern US populations for comparison, and (c genotyping a subset of samples at 15,698 SNPs. Major results are: (1 Northern and Southern CA populations are distinct. (2 Northern populations are more genetically diverse than Southern CA populations. (3 Northern and Southern CA groups were likely founded by two independent introductions which came from the South Central US and Southwest US/northern Mexico regions respectively. (4 Our genetic data suggest that the founding events giving rise to the Northern CA and Southern CA populations likely occurred before the populations were first recognized in 2013 and 2014, respectively. (5 A Northern CA population analyzed at multiple time-points (two years apart is genetically stable, consistent with permanent in situ breeding. These results expand previous work on the origin of California A. aegypti with the novel finding that this species entered California on multiple occasions, likely some years before its initial detection. This work has implications for mosquito surveillance and vector control activities not only in California but also in other regions where the distribution of this invasive mosquito is expanding.

  10. Insecticide resistance status of Aedes aegypti (L.) from Colombia.

    Science.gov (United States)

    Fonseca-González, Idalyd; Quiñones, Martha L; Lenhart, Audrey; Brogdon, William G

    2011-04-01

    To evaluate the insecticide susceptibility status of Aedes aegypti (L.) in Colombia, and as part of the National Network of Insecticide Resistance Surveillance, 12 mosquito populations were assessed for resistance to pyrethroids, organophosphates and DDT. Bioassays were performed using WHO and CDC methodologies. The underlying resistance mechanisms were investigated through biochemical assays and RT-PCR. All mosquito populations were susceptible to malathion, deltamethrin and cyfluthrin, and highly resistant to DDT and etofenprox. Resistance to lambda-cyhalothrin, permethrin and fenitrothion ranged from moderate to high in some populations from Chocó and Putumayo states. In Antioquia state, the Santa Fe population was resistant to fenitrothion. Biochemical assays showed high levels of both cytochrome P450 monooxygenases (CYP) and non-specific esterases (NSE) in some of the fenitrothion- and pyrethroid-resistant populations. All populations showed high levels of glutathione-S-transferase (GST) activity. GSTe2 gene was found overexpressed in DDT-resistant populations compared with Rockefeller susceptible strain. Differences in insecticide resistance status were observed between insecticides and localities. Although the biochemical assay results suggest that CYP and NSE could play an important role in the pyrethroid and fenitrothion resistance detected, other mechanisms remain to be investigated, including knockdown resistance. Resistance to DDT was high in all populations, and GST activity is probably the main enzymatic mechanism associated with this resistance. The results of this study provide baseline data on insecticide resistance in Colombian A. aegypti populations, and will allow comparison of changes in susceptibility status in this vector over time. Copyright © 2011 Society of Chemical Industry.

  11. Seasonal Synchronization of Diapause Phases in Aedes albopictus (Diptera: Culicidae).

    Science.gov (United States)

    Lacour, Guillaume; Chanaud, Lionel; L'Ambert, Grégory; Hance, Thierry

    2015-01-01

    In temperate areas, population dynamics of the invasive Asian tiger mosquito Aedes albopictus are strongly affected by winter. The work we present here analyzes the adaptive synchronization of the diapause process in the wintry generation of A. albopictus, where the egg stage is exposed to adverse winter conditions. The seasonal pattern of egg laying activity of a French Mediterranean population of the Asian tiger mosquito was monitored weekly for 2 years with ovitraps. The field diapause incidence and the critical photoperiod (CPP, i.e. the maternal day length inducing diapause in 50% of the eggs), were determined by hatching experiments on the collected eggs. The period of diapause termination was estimated by a field survey of the first hatchings for both years. The CPP is equal to 13.5 hours of light and occurs in the field on the 25th of August. Thus, it is on September 11th, 17 days after the CPP, that 50% of the eggs are in a prediapause stage in the field. The egg diapause rate increases rapidly during September, whereas the mean number of eggs laid decreases sharply after mid-September. Surprisingly, after having reached a peak of 95% at the end of September, from mid-October the diapause incidence declined and stayed below 50%. Indeed, both years the diapause initiates before the rapid decrease of the environmental temperature. This leaves a sufficient period of time to the complete development of one generation of A. albopictus with effective induction of diapause in the laid eggs. The very first larvae hatched were sampled both years in the first half of March. With 20 to 26 weeks in the egg stage and about 7 weeks in the larval stages, the first annual generation spends a long time in immature stages. On a practical point of view, this long development time represents a wide window for eggs and larvae control in early spring.

  12. Effects of mineralogy on sorption of strontium and cesium onto Calico Hills Tuff

    International Nuclear Information System (INIS)

    Meyer, R.E.; Arnold, W.D.; Case, F.I.; O'Kelley, G.D.; Land, J.F.

    1990-04-01

    The sorption properties of tuff formations at the proposed site for the high-level nuclear waste repository at Yucca Mountain, Nevada, have been extensively studied. Sorption and desorption measurements were made of strontium and cesium onto clinoptilolite and Calico Hills Tuff. The object was to see whether there was a correlation between sorption of strontium and cesium onto Calico Hills Tuff and the sorption of strontium and cesium onto clinoptilolite based on the content of clinoptilolite in the Calico Hills Tuff. 13 refs., 10 figs., 6 tabs

  13. Aedes (Stegomyia) aegypti and Aedes (Howardina) cozumelensis in Yucatán State, México, with a summary of published collection records for Ae. cozumelensis.

    Science.gov (United States)

    García-Rejón, Julián E; López-Uribe, Mildred P; Loroño-Pino, María Alba; Arana-Guardia, Roger; Puc-Tinal, Maria; López-Uribe, Genny M; Coba-Tún, Carlos; Baak-Baak, Carlos M; Machain-Williams, Carlos; Reyes-Solis, Guadalupe C; Lozano-Fuentes, Saul; Saavedra-Rodriguez, Karla; Black, William C; Beaty, Barry J; Eisen, Lars

    2012-12-01

    We collected mosquito immatures from artificial containers during 2010-2011 from 26 communities, ranging in size from small rural communities to large urban centers, located in different parts of Yucatán State in southeastern México. The arbovirus vector Aedes (Stegomyia) aegypti was collected from all 26 examined communities, and nine of the communities also yielded another container-inhabiting Aedes mosquito: Aedes (Howardina) cozumelensis. The communities from which Ae. cozumelensis were collected were all small rural communities (holes as well as various artificial containers including metal cans, flower vases, buckets, tires, and a water storage tank. The co-occurrence with Ae. aegypti in small rural communities poses intriguing questions regarding linkages between these mosquitoes, including the potential for direct competition for larval development sites. Additional studies are needed to determine how commonly Ae. cozumelensis feeds on human blood and whether it is naturally infected with arboviruses or other pathogens of medical or veterinary importance. We also summarize the published records for Ae. cozumelensis, which are restricted to collections from México's Yucatán Peninsula and Belize, and uniformly represent geographic locations where Ae. aegypti can be expected to occur. © 2012 The Society for Vector Ecology.

  14. First isolation of Aedes flavivirus in the Western Hemisphere and evidence of vertical transmission in the mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae)

    Energy Technology Data Exchange (ETDEWEB)

    Haddow, Andrew D., E-mail: adhaddow@gmail.com [Institute for Human Infections and Immunity, Center for Emerging Infectious Diseases and Biodefense, Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609 (United States); Guzman, Hilda; Popov, Vsevolod L. [Institute for Human Infections and Immunity, Center for Emerging Infectious Diseases and Biodefense, Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609 (United States); Wood, Thomas G.; Widen, Steven G. [Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555-0609 (United States); Haddow, Alastair D. [Mercy Clinic, 2115 S. Fremont, Springfield, MO 65804 (United States); Tesh, Robert B.; Weaver, Scott C. [Institute for Human Infections and Immunity, Center for Emerging Infectious Diseases and Biodefense, Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609 (United States)

    2013-06-05

    We report here the first evidence of vertical transmission of Aedes flavivirus (AEFV) and its first isolation in the Western Hemisphere. AEFV strain SPFLD-MO-2011-MP6 was isolated in C6/36 cells from a pool of male Aedes albopictus mosquitoes that were reared to adults from larvae collected in southwest Missouri, USA, in 2011. Electron micrographs of the virus showed virions of approximately 45 nm in diameter with morphological characteristics associated with flaviviruses. The genomic sequence demonstrated that AEFV-SPFLD-MO-2011-MP6 shares a high degree of nucleotide and amino acid sequence identity with the AEFV Narita-21 strain, isolated in Japan in 2003. Intracerebral inoculation of newborn mice with the virus failed to produce observable illness or death and the virus did not replicate in vertebrate cells, consistent with a lack of vertebrate host range. - Highlights: ► The first report of Aedes flavivirus (AEFV) in the Western Hemisphere. ► The first evidence of vertical transmission of AEFV in mosquitoes. ► The first electron micrograph of AEFV. ► The first attempt to infect animals with AEFV.

  15. First isolation of Aedes flavivirus in the Western Hemisphere and evidence of vertical transmission in the mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae)

    International Nuclear Information System (INIS)

    Haddow, Andrew D.; Guzman, Hilda; Popov, Vsevolod L.; Wood, Thomas G.; Widen, Steven G.; Haddow, Alastair D.; Tesh, Robert B.; Weaver, Scott C.

    2013-01-01

    We report here the first evidence of vertical transmission of Aedes flavivirus (AEFV) and its first isolation in the Western Hemisphere. AEFV strain SPFLD-MO-2011-MP6 was isolated in C6/36 cells from a pool of male Aedes albopictus mosquitoes that were reared to adults from larvae collected in southwest Missouri, USA, in 2011. Electron micrographs of the virus showed virions of approximately 45 nm in diameter with morphological characteristics associated with flaviviruses. The genomic sequence demonstrated that AEFV-SPFLD-MO-2011-MP6 shares a high degree of nucleotide and amino acid sequence identity with the AEFV Narita-21 strain, isolated in Japan in 2003. Intracerebral inoculation of newborn mice with the virus failed to produce observable illness or death and the virus did not replicate in vertebrate cells, consistent with a lack of vertebrate host range. - Highlights: ► The first report of Aedes flavivirus (AEFV) in the Western Hemisphere. ► The first evidence of vertical transmission of AEFV in mosquitoes. ► The first electron micrograph of AEFV. ► The first attempt to infect animals with AEFV

  16. Determination of Insecticidal Effect (LC50 and LC90) of Organic Fatty Acids Mixture (C8910+Silicone) Against Aedes aegypti and Aedes albopictus (Diptera: Culicidae).

    Science.gov (United States)

    Dunford, James C; Falconer, Aneika; Leite, Laura N; Wirtz, Robert A; Brogdon, William G

    2016-05-01

    Emerging and re-emerging vector-borne diseases such as chikungunya and dengue and associated Aedes vectors are expanding their historical ranges; thus, there is a need for the development of novel insecticides for use in vector control programs. The mosquito toxicity of a novel insecticide and repellent consisting of medium-chain carbon fatty acids (C8910) was examined. Determination of LC 50 and LC 90 was made against colony-reared Aedes aegypti (L.) and Aedes albopictus (Skuse) using probit analysis on mortality data generated by Centers for Disease Control and Prevention bottle bioassays. Six different concentrations of C8910 + silicone oil yielded an LC 50 of 160.3 µg a.i/bottle (147.6-182.7) and LC 90 of 282.8 (233.2-394.2) in Ae. aegypti; five concentrations yielded an LC 50 of 125.4 (116.1-137.6) and LC 90 of 192.5 (165.0-278.9) in Ae. albopictus. Further development of C8910 and similar compounds could provide vector control specialists novel insecticides for controlling insect disease vectors. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  17. Geospatial analysis of invasion of the Asian tiger mosquito Aedes albopictus: competition with Aedes japonicus japonicus in its northern limit area in Japan

    Directory of Open Access Journals (Sweden)

    Naoko Nihei

    2014-05-01

    Full Text Available The mosquito Aedes albopictus, indigenous to Southeast Asia and nearby islands, has spread almost worldwide during recent decades. We confirm the invasion of this mosquito, first reported in Yamagata city in northeast Honshu, Japan in 2000. Previously, only Ae. japonicus japonicus had been collected in this place, but 2 years later, the population of Ae. albopictus had increased, so more than 80% of the total number of larval colonies there consisted of this species. In contrast to Yamagata’s new residential area, now infested by Ae. albopictus, the original mosquito remains in the city but its habitats are generally closer to the surrounding mountains, where the normalized difference vegetation index is higher. The factors affecting the distribution of both species in Yamagata city were studied using geographical information systems (GIS based on data derived from field surveys, aerial photographs, satellite images and digital maps. The range of Aedes mosquito habitats was estimated and visualised on polygon maps and no significant differences were noted when the polygon area was calculated by GIS software in comparison with the satellite images. Although Ae. j. japonicus was expected to be rapidly overrun by Ae. albopictus, this did not happen. Currently, both species coexist; not only in separate sites, but also simultaneously in various water bodies, where larvae from both species have frequently been seen. However, the competitive relationship between these two Aedes species within a warming environment is an issue that should be closely monitored.

  18. Factors influencing the development of Plasmodium gallinaceum in Aedes fluviatilis

    Directory of Open Access Journals (Sweden)

    Mariana V. Tasón de Camargo

    1983-03-01

    Full Text Available Aedes fluviatilis is susceptible to infection by Plasmodium gallinaceum and is a convenient insect host for the malaria parasite in countries where Aedees aegypti cannot be maintained in laboratories. In South America, for instance, the rearing of A. aegypti the main vector of urban yellow fever, is not advaisable because of the potential health hazard it represents. Our results of the comparative studies carried out between the sporogonic cycle produced with two lines of P. gallinaceum parasites into A. fuviatilis were as follows. As proved for A. aegypti, mosquito infection rates were variable when A. fluviatilis blood-fed on chicks infected with and old syringe-passaged strain of P. gallinaceum. Oocysts developed in 41% of those mosquitos and the mean peak of oocyst production was 56 per stomach. Salivary gland infections developed in about 6% of the mosquitos. The course of sporogony was unrelated to the size of the inoculum administered to chicks or to the route by which the birds were infected. The development of infected salivary glands was unrelated to oocyst production. Sporogony of P. gallinaceum was more uniform when mosquitos blood-fed on chicks infected with a sporozoite-passaged strain. Oocysts developed in about 50% of those mosquitoes and the mean peak of oocyst production was 138 per stomach, with some individuals having as many as 600-800 oocysts. Infected salivary glands developed in a mean of 27% of the mosquitos but, in some batches, was a high as 50%. Patterns of salivary gland parasitism were similar to those of oocyst production. The course of sporogony of P. gallinaceum in A. fluviatilis is analized in relation to degree of parasitemia and gametocytemia in the vertebrate host.Aedes fluviatilis é susceptível à infecção por Plasmodium gallinaceum, sendo considerado um modelo experimental útil para esta infecção em regiões nas quais Aedes aegypti não deve ser criado em laboratório, por razões de segurança. Na

  19. Geodynamic modelling of the Broken Hill mineralising system

    International Nuclear Information System (INIS)

    Hobbs, B.; Walshe, J.; Ord, A.; Zhang, Y.; Zhao, C.

    1999-01-01

    Full text: The origin of the Broken Hill ore body is the topic of considerable controversy and there are at least seven ore genesis models in vogue (Parr and Plimer, 1993). Unfortunately none of the data are definitive with respect to one model or another and progress in the area is hindered by protagonists adhering to their pet model by inventing processes that enable a problematic data set to fit their particular model. We attempt here to overcome some of these difficulties by adopting a very simplistic approach and assume that each data set is to be interpreted at face value with no attempt to distort the data so that it fits some predetermined philosophy. The data sets to be taken at face value are: (i) The carbon isotopic data which are ambiguous and can be taken to indicate either a source of carbon from organic sources and/or from deep crustal/mantle sources. (ii) The sulphur isotopic data which indicate a plutonic/hydrothermal source for the sulphur. (iii) The lead isotopic data which indicate a crustal source for the lead but perhaps with some mixing with a mantle source. (iv) The lead model age which indicates an age for the mineralisation ( 1675 Ma), 15 million years younger than the SHRIMP U-Pb ages for the host sediments (1690 Ma). However the errors are such that an origin synchronous with sedimentation is still possible. In the form of model constraints, we also assume that Broken Hill Type mineralisation is a true type, namely, Pb/Zn mineralisation that occurs in what are now amphibolite to granulite grades of metamorphism. This observation implies that the mineralisation is spatially and temporally associated with high grade metamorphism or that the site of mineralisation is associated with diagenesis or low grade metamorphism but is such that late in the geotectonic history that site is predestined to undergo high grade metamorphism. Since the only experimental data available on Pb/Zn solubilities involves oxidised fluids we are forced to develop

  20. Ovicidal activity of Metarhizium brunneum (Mb F52) on dengue fever vector, Aedes aegypti

    Science.gov (United States)

    The ovicidal activity of Metarhizium brunneum F52 (Mb F52) grown from granules was evaluated against Aedes aegypti eggs over time. Survival of larvae from treated eggs was significantly less when compared with untreated eggs at 7, 10 and 14 days post treatment. Only 27 % of treated eggs produced vi...

  1. Evaluation of bifenthrin applications in tires to prevent Aedes mosquito breeding.

    Science.gov (United States)

    Nguyen, Huy T; Whelan, Peter I; Shortus, Matthew S; Jacups, Susan P

    2009-03-01

    The efficacy of maximum label rates of bifenthrin applications to dry tires to prevent Aedes mosquito breeding was investigated by field colonization and bioassay trials in shaded and unshaded locations. Aedes notoscriptus and Culex quinquefasciatus larvae were the most abundant species present in the field colonization trial. Colonization and survival of Ae. notoscriptus larvae to the late instar occurred significantly earlier in treated tires in shaded compared with unshaded locations (P = 0.002). Bifenthrin applications in shaded tires only prevented early instar survival for approximately 2.6 wk. Aedes notoscriptus late instars did not appear in the treated unshaded tires. Culex quinquefasciatus colonized treated tires from the 2nd wk in both shaded and unshaded treatments. In the bioassay, water from bifenthrin-treated tires, through extrapolation, was found to kill approximately 100% of late instar Ae. notoscriptus for only approximately 2.0-2.2 wk in shaded and unshaded tires. Under conditions optimal for Aedes breeding, such as shaded locations, high ambient temperatures, high relative humidity, and high amounts of leaf/organic matter accumulations, bifenthrin may not be effective as a larval control measure in tires for greater than 2.0-2.6 wk.

  2. Aedes (Stegomyia) albopictus (Skuse), a potential new Dengue vector in southern Cameroon.

    OpenAIRE

    Fontenille, D.; Toto, J. C.

    2001-01-01

    Aedes albopictus, a mosquito vector of Dengue virus, has been recorded for the first time in Cameroon. Entomologic surveys in 2000 demonstrated that it is widespread in southern Cameroon, colonizing a wide variety of breeding sites and biting humans in every district surveyed. The presence of this vector increases the risk for emergence of dengue in Cameroon.

  3. The tale of two buckets and associated containers: impact on aedes albopictus oviposition

    Science.gov (United States)

    Aedes albopictus is an invasive species. Its oviposition behavior is the subject of several projects in our research unit. The main emphasis of this presentation is a study which utilizes two five gallon buckets, one heated and one with ambient temperature. The heat is provided by an aquarium hea...

  4. High Resolution Spatial Analysis of Habitat Preference of Aedes Albopictus (Diptera: Culicidae) in an Urban Environment

    NARCIS (Netherlands)

    Cianci, D.; Hartemink, N.; Zeimes, C.B.; Vanwambeke, S.O.; Ienco, A.; Caputo, B.

    2015-01-01

    Over the past decades, the Asian tiger mosquito (Aedes albopictus (Skuse, 1895)) has emerged in many countries, and it has colonized new environments, including urban areas. The species is a nuisance and a potential vector of several human pathogens, and a better understanding of the habitat

  5. Occurrence and distribution of Aedes albopictus (Skuse) in the Netherlands; survey 2006-2007 : report

    NARCIS (Netherlands)

    Scholte, E.J.; Takken, W.

    2007-01-01

    In the summer of 2005, the Asian tigermosquito (Aedes albopictus) was found for the first time in the Netherlands. It was intercepted in several horticultural companies that import the ornamental plant Lucky bamboo from southern China, an endemic area for this mosquito species. The current report

  6. Transgenic expression of the Aedes aegypti CYP9J28 confers pyrethroid resistance in Drosophila melanogaster

    NARCIS (Netherlands)

    Pavlidi, N.; Monastirioti, M.; Daborn, P.; Van Leeuwen, T.; Vontas, J.

    2012-01-01

    The emergence and spread of insecticide resistance in mosquitoes, such as the major vector of dengue and yellow fever Aedes aegypti, is a major public health problem. A number of studies have been conducted to-date aiming to identify specific molecular changes that are associated with the phenotype,

  7. REPELLENCY OF ESSENTIAL OIL OF PIPER ADUNCUM AGAINST AEDES ALBOPICTUS IN THE LABORATORY

    NARCIS (Netherlands)

    Misni, Norashiqin; Sulaiman, Sallehudin; Othman, Hidayatulfathi; Omar, Baharudin

    2009-01-01

    The repellent activity of Piper aduncum essential oil against Aedes albopictus was investigated under laboratory conditions with human volunteers. The lowest median effective dose (ED(50)) value was 1.5 mu g/cm(2) at 60 sec of exposure when compared to 90 see (2.1 mu g/cm(2)) and 120 see (1.8 mu

  8. Evaluation of pyriproxyfen dissemination via Aedes albopictus from a point source larvicide application in northeast Florida

    Science.gov (United States)

    The Asian tiger mosquito, Aedes albopictus, ranks among the most important vectors of dengue fever, Zika virus, and chikungunya virus. With no specific medications or vaccines available, vector control is the only way to combat these diseases. Autodissemination of the insect growth regulator pyripro...

  9. Synthesis and Mosquitocidal Activity of a Series of Hydrazone Derivatives against Aedes aegypti

    Science.gov (United States)

    Background: Aedes aegypti is an important mosquito vector for the transmission of several infectious diseases. Current insecticides play a vital role in controlling mosquitoes; however, the frequent use of insecticides has led to the development of insecticide resistance. In order to control mosquit...

  10. Spatial repellency screening in a high-throughput apparatus with Aedes aegypti and Anopheles gambiae

    Science.gov (United States)

    Spatial repellents are essential for personal protection against mosquitoes, such as Aedes aegypti and Anopheles gambiae, to reduce annoyance biting and transmission of mosquito-borne diseases. The number of safe and effective repellents, including DEET, picaridin, and IR3535, is limited and contin...

  11. Identification of insecticidal principals from cucumber seed oil against the yellow fever mosquito, Aedes aegypti

    Science.gov (United States)

    The yellow fever mosquito, Aedes aegypti, is one of the most medically important mosquito species due to its ability to spread viruses of yellow fever, dengue fever and Zika in humans. In this study, the insecticidal activity of seventeen plant essential oils were evaluated to toxicity by topical a...

  12. A MIDGUT DIGESTIVE PHOSPHOLIPASE A2 IN LARVAL MOSQUITOES, AEDES ALBOPICTUS AND CULEX QUINQUEFASCIATUS

    Science.gov (United States)

    Phospholipase A2 (PLA2) is a secretory digestive enzyme that hydrolyzes ester bond at sn-2 position of dietary phospholipids, creating free fatty acid and lysophopholipid. The free fatty acids (arachidonic acid) are absorbed into midgut cells. Aedes albopictus and Culex quinquefasciatus digestive PL...

  13. Wingbeat frequency-sweep and visual stimuli for trapping male Aedes aegypti (Diptera: Culicidae)

    Science.gov (United States)

    Combinations of female wingbeat acoustic cues and visual cues were evaluated to determine their potential for use in male Aedes aegypti (L.) traps in peridomestic environments. A modified Centers for Disease control (CDC) light trap using a 350-500 Hz frequency-sweep broadcast from a speaker as an a...

  14. Vertical Transmission of Zika Virus by Aedes aegypti and Ae. albopictus Mosquitoes.

    Science.gov (United States)

    Ciota, Alexander T; Bialosuknia, Sean M; Ehrbar, Dylan J; Kramer, Laura D

    2017-05-01

    To determine the potential role of vertical transmission in Zika virus expansion, we evaluated larval pools of perorally infected Aedes aegypti and Ae. albopictus adult female mosquitoes; ≈1/84 larvae tested were Zika virus-positive; and rates varied among mosquito populations. Thus, vertical transmission may play a role in Zika virus spread and maintenance.

  15. Technologies to Combat Aedes Mosquitoes: A Model Based on Smart City.

    Science.gov (United States)

    de Souza Silva, Geovanna Cristine; Peltonen, Laura-Maria; Pruinelli, Lisiane; Yoshikazu Shishido, Henrique; Jacklin Eler, Gabrielle

    2018-01-01

    Aedes aegypti and Aedes albopictus mosquitoes are responsible for the transmission of diseases such as dengue fever, yellow fever, chikungunya fever, zika virus fever, some of which can cause irreversible central nervous system problems and death. This study investigates what technologies are being used for combatting and monitoring the Aedes mosquitoes and to propose joining these technologies into a single and complete solution using the Smart Cities concept. A search for newscasts on Google and mobile apps in app stores were performed to identify technological solutions for combat to Aedes mosquitoes. Also, a model for joint technology was proposed. Results identified the following technologies: 170 software, two sensors, two drones, one electronic device, ten mosquito traps/lures, seven biological tools, six biotechnologies, and eight chemical formulations. Technological resources and adoption of preventive measures by the population could be a useful method for the mosquito control. Examples include a georeferenced model for identification and examination of larvae, application of chemical/biological products, real-time mapping, sending of educational materials via email or social media for the population, and alerts to health professionals in the zones of combat/risk. In combination, these technologies may indicate a better solution to the current problem.

  16. Ovicidal activity of Ageratina adenophora (Family: Asteraceae) against dengue vector, Aedes aegypti (Diptera: Culicidae)

    Science.gov (United States)

    To determine the ovicidal efficacy of different solvent leaf extracts of Ageratina adenophora against dengue vector Aedes aegypti . Methods: The ovicidal efficacy of the crude leaf extracts of A. adenophora with five different solvents (hexane, benzene, chloroform, ethyl acetate, methanol) and was ...

  17. Resistance to deltamethrine in two populations of Aedes aegypti (Diptera, Culicidae from Peru

    Directory of Open Access Journals (Sweden)

    Julio Chávez

    2013-05-01

    Full Text Available The objective of the present work was to determine the resistance levels to deltamethrine in two populations of Aedes aegypti from Peru. Bioassays in adults were carried out following the methodology of the World Health Organization. We met resistance in the Sullana population with 70% of mortality and susceptibility in the population The Future El Porvenir with 99% of mortality.

  18. An invasive mosquito species Aedes albopictus found in the Czech Republic, 2012

    Czech Academy of Sciences Publication Activity Database

    Šebesta, Oldřich; Rudolf, Ivo; Betášová, Lenka; Peško, Juraj; Hubálek, Zdeněk

    2012-01-01

    Roč. 17, č. 43 (2012), s. 20301 ISSN 1560-7917 Institutional support: RVO:68081766 Keywords : invasive mosquito species * Aedes albopictus Subject RIV: EG - Zoology Impact factor: 5.491, year: 2012 http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20301

  19. Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Rudolf, Ivo; Šebesta, Oldřich; Mendel, Jan; Betášová, Lenka; Bocková, E.; Jedličková, Petra; Venclíková, Kristýna; Blažejová, Hana; Šikutová, Silvie; Hubálek, Zdeněk

    2014-01-01

    Roč. 113, č. 12 (2014), s. 4663-4667 ISSN 0932-0113 EU Projects: European Commission(XE) 261504 - EDENEXT Institutional support: RVO:68081766 Keywords : Aedes vexans * Mosquito vectors * Dirofilaria repens * Dogs * Zoonotic dirofilariosis * Setaria spp. Subject RIV: EG - Zoology Impact factor: 2.098, year: 2014

  20. Infection of adult Aedes aegypti and Ae. albopictus mosquitoes with the entomopathogenic fungus Metarhizium anisopliae

    NARCIS (Netherlands)

    Scholte, E.J.; Takken, W.; Knols, B.G.J.

    2007-01-01

    This study describes a laboratory investigation on the use of the insect-pathogenic fungus Metarhizium anisopliae against adult Aedes aegypti and Ae. albopictus mosquitoes. At a dosage of 1.6 × 1010 conidia/m2, applied on material that served as a mosquito resting site, an average of 87.1 ± 2.65% of

  1. [Behavior of immatures Aedes aegypti in the coast State of São Paulo, Brazil].

    Science.gov (United States)

    Glasser, Carmen Moreno; Arduino, Marylene de Brito; Barbosa, Gerson Laurindo; Ciaravolo, Ricardo Mario de Carvalho; Domingos, Maria de Fátima; Oliveira, Cleide Dantas; Pereira, Marisa; Silva, Marcos; Trevisan, Alexandra Myuki Yoshioka

    2011-01-01

    In a region of high dengue incidence, on the coast of the State of São Paulo, 9 areas were selected to evaluate the behavior of immature Aedes aegypti. The 9 areas were grouped into 4 strata according to soil use and occupation. Larvae and pupas were collected in a sample of approximately 500 buildings in each area. Although tires and canvas presented the highest positive rates for Aedes aegypti, drains and other containers fixed to the buildings were highly predominant among positive containers; 32 to 76% of the positive containers in the 4 study strata. Public areas of apartment buildings and large non-residential premises presented the highest positive rates for Aedes Aegypti, while apartments presented the lowest. Infestation levels were greater in residential areas with predominance of apartment buildings, where 76% of the breeding sites detected were containers fixed to the buildings. This knowledge is an important tool in the control strategy, since it reinforces the need for special attention regarding certain types of buildings and the adjustment of technical norms for pluvial water drains and improvement of building maintenance. Moreover, systematic observations are required to follow-up the occupancy dynamic of different buildings and containers by Aedes aegypti and the incorporation of this knowledge in the control of vectors in the region.

  2. Pengaruh Perbedaan Warna Ovitrap terhadap Jumlah Telur Nyamuk Aedes spp yang Terperangkap

    Directory of Open Access Journals (Sweden)

    Anif Budiyanto

    2013-02-01

    Full Text Available Abstract. The main of Aedes sp breeding site are water containers in or around the home or public places. Container or vessel that is used daily by people, such as flower vase, bird drinks, ant tra ps, etc. that can become a breeding place of mosquitoes Aedes sp, has a varie­ty of colors. It has been studied with the aim of knowing the influence of color dijJerence ovitrap to the number of eggs of Aedes sp This study used 25 ovitrap is divided into 5 groups consisting of red, yellow, blue, black and white. Each group ovitrap placed in houses and left for 5 days. This activity is carried out up to 8 times repetition, so that the total sample numbered 200. From dijJerent test two proportions is known that there are significant dijJerences from their respective mean number of eggs of dijJerent colors of ovitrap. And the results of multiple comparison analysis, it is known that the mean number of eggs of red and black color of ovitrap significantly dijJerent when paired with the mean number of eggs from ovitrap a yel­low, blue and white.Keywords: breeding site, Aedes, color of ovitrap, ovitrap

  3. Geologic map of the northern White Hills, Mohave County, Arizona

    Science.gov (United States)

    Howard, Keith A.; Priest, Susan S.; Lundstrom, Scott C.; Block, Debra L.

    2017-07-10

    IntroductionThe northern White Hills map area lies within the Kingman Uplift, a regional structural high in which Tertiary rocks lie directly on Proterozoic rocks as a result of Cretaceous orogenic uplift and erosional stripping of Paleozoic and Mesozoic strata. The Miocene Salt Spring Fault forms the major structural boundary in the map area. This low-angle normal fault separates a footwall (lower plate) of Proterozoic gneisses on the east and south from a hanging wall (upper plate) of faulted middle Miocene volcanic and sedimentary rocks and their Proterozoic substrate. The fault is part of the South Virgin–White Hills Detachment Fault, which records significant tectonic extension that decreases from north to south. Along most of its trace, the Salt Spring Fault dips gently westward, but it also has north-dipping segments along salients. A dissected, domelike landscape on the eroded footwall, which contains antiformal salients and synformal reentrants, extends through the map area from Salt Spring Bay southward to the Golden Rule Peak area. The “Lost Basin Range” represents an upthrown block of the footwall, raised on the steeper Lost Basin Range Fault.The Salt Spring Fault, as well as the normal faults that segment its hanging wall, deform rocks that are about 16 to 10 Ma, and younger deposits overlie the faults. Rhyodacitic welded tuff about 15 Ma underlies a succession of geochemically intermediate to progressively more mafic lavas (including alkali basalt) that range from about 14.7 to 8 Ma, interfingered with sedimentary rocks and breccias in the western part of the map area. Upper Miocene strata record further filling of the extension-formed continental basins. Basins that are still present in the modern landscape reflect the youngest stages of extensional-basin formation, expressed as the downfaulted Detrital Valley and Hualapai Wash basins in the western and eastern parts of the map area, respectively, as well as the north-centrally located

  4. NORTH HILL CREEK 3-D SEISMIC EXPLORATION PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    Marc T. Eckels; David H. Suek; Denise H. Harrison; Paul J. Harrison

    2004-05-06

    Wind River Resources Corporation (WRRC) received a DOE grant in support of its proposal to acquire, process and interpret fifteen square miles of high-quality 3-D seismic data on non-allotted trust lands of the Uintah and Ouray (Ute) Indian Reservation, northeastern Utah, in 2000. Subsequent to receiving notice that its proposal would be funded, WRRC was able to add ten square miles of adjacent state and federal mineral acreage underlying tribal surface lands by arrangement with the operator of the Flat Rock Field. The twenty-five square mile 3-D seismic survey was conducted during the fall of 2000. The data were processed through the winter of 2000-2001, and initial interpretation took place during the spring of 2001. The initial interpretation identified multiple attractive drilling prospects, two of which were staked and permitted during the summer of 2001. The two initial wells were drilled in September and October of 2001. A deeper test was drilled in June of 2002. Subsequently a ten-well deep drilling evaluation program was conducted from October of 2002 through March 2004. The present report discusses the background of the project; design and execution of the 3-D seismic survey; processing and interpretation of the data; and drilling, completion and production results of a sample of the wells drilled on the basis of the interpreted survey. Fifteen wells have been drilled to test targets identified on the North Hill Creek 3-D Seismic Survey. None of these wildcat exploratory wells has been a dry hole, and several are among the best gas producers in Utah. The quality of the data produced by this first significant exploratory 3-D survey in the Uinta Basin has encouraged other operators to employ this technology. At least two additional 3-D seismic surveys have been completed in the vicinity of the North Hill Creek Survey, and five additional surveys are being planned for the 2004 field season. This project was successful in finding commercial oil, natural gas

  5. Sobre la muerte voluntaria

    Directory of Open Access Journals (Sweden)

    Juan Álvarez-Cienfuegos Fidalgo

    2012-03-01

    Full Text Available El problema de cómo morir en nuestra sociedad se ha convertido en una inquietud para los ciudadanos y la reflexión moral. Especialmcnte por dos razones: por el desarrollo de la tecnología médica y por el hecho de que la mayor parte de los fallecimientos tienen lugar fuera del hogar, donde tradicionalmente ocurra el deceso. El presente artículo tiene como objetivo acercarse a esta cuestión. Para ello, se parte de la autonomía del ciudadano, con derecho a decidir sobre lo que considera cuál sea su manera de vivir y de morir. A tal fin, se hace una breve introducción sobre el dolor, el suicidio y la muerte, desde un punto de vista antropológico, pasando, después, a mostrar las cuestiones relacionadas con el problema de la eutanasia, donde la atención se dirigirá, ante todo, al enjuiciamiento crítico del tenso equilibrio en el que se mantienen dos principios fundamentales de la bioética: el de no maleficencia y el de autonomía del ciudadano.The problem of how to die in modern society has become a concern for both the citizen and moral reflection for two reasons in particular: due to developments in medical technology, and because most deaths occur outside the honre, where they traditionaÍlY took place. This paper addresses this issue, starting from citizen autonomy, the right to decide on what s/he considers to be his or her way of living and dying. To this end, a brief introduction considers pain, suicide and death from an anthropological viewpoint. Questions related to the problem of euthanasia are then exposed, with particular attention to, above all the critical judgement of the tense balance in which two fundamental principies of bioethics are held, the principle of non-maleficence and that of citizen autonomy.

  6. Potensi Serbuk Daun Sirih (piper betle, Linn Sebagai Larvasida Nyamuk Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Betriyon Betriyon

    2017-02-01

    Full Text Available Abstract Larvacide was compound/essence used to kill  larvae stadium. Many people used Piper betle to traditional medicine. Piper betle plant L,.has contents bioactive compound as flavonoid compound, atsiri volatile, polifenol, tannin, alkaloid and saponin which have quality as larvacide. Based on the case above, this research aim to put the experiment Piper betle of the Aedes aegypti mosquito instars IV. This was pure experiment research using complete random design. This research was done at laboratory . Larva used was  Aedes aegypti mosquito instars IV. Concentration used of this research was : 0,1; 0,2; 0,3; 0,4; 05 percent b/v. This research has control (+ was temephos 0,0001 persen, control (- was aquades water not added anything. Observation done every hour up to all the larva death with the replication 5 times. Larva death cumulative data on 10th hour and 24 th used to calculate LC50 and LT50 used probit regression analysis than data analyzed using Levene’s test. The result of this research showed that the cost of LC50 piper betle plant L. on 10th hour was 0,54 ± 0,147 persen b/v, than on 24 hour LC50 on concentration 0,07 up to 0,28 percent b/v. the time needed to cross out 50 percent Aedes aegypti instar IV of the concentration 0,05 percent time needed 19. Based on a probit analysis of the relationship between the level of concentration with the number of larvae mortality, Piper betle have activities as larvacide of Aedes aegypti. Keywods: piper betle leaf powder, larvacide, Aedes aegyti AbstrakLarvasida adalah senyawa/zat yang digunakan untuk membunuh stadium larva. Tanaman Piper betle, Linn. (sirih hijau sudah banyak dimanfaatkan oleh kalangan masyarakat sebagai obat tradisional. Daun sirih hijau memiliki kandungan senyawa bioaktif seperti senyawa flavonoid, minyak atsiri, polifenol, tannin, alkaloid dan saponin yang dapat bersifat sebagai larvasida. Penelitian ini bertujuan untuk mengetahui manfaat daun sirih hijau yaitu

  7. Modeling the Environmental Suitability for Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus (Diptera: Culicidae) in the Contiguous United States.

    Science.gov (United States)

    Johnson, Tammi L; Haque, Ubydul; Monaghan, Andrew J; Eisen, Lars; Hahn, Micah B; Hayden, Mary H; Savage, Harry M; McAllister, Janet; Mutebi, John-Paul; Eisen, Rebecca J

    2017-11-07

    The mosquitoes Aedes (Stegomyia) aegypti (L.)(Diptera:Culicidae) and Ae. (Stegomyia) albopictus (Skuse) (Diptera:Culicidae) transmit dengue, chikungunya, and Zika viruses and represent a growing public health threat in parts of the United States where they are established. To complement existing mosquito presence records based on discontinuous, non-systematic surveillance efforts, we developed county-scale environmental suitability maps for both species using maximum entropy modeling to fit climatic variables to county presence records from 1960-2016 in the contiguous United States. The predictive models for Ae. aegypti and Ae. albopictus had an overall accuracy of 0.84 and 0.85, respectively. Cumulative growing degree days (GDDs) during the winter months, an indicator of overall warmth, was the most important predictive variable for both species and was positively associated with environmental suitability. The number (percentage) of counties classified as environmentally suitable, based on models with 90 or 99% sensitivity, ranged from 1,443 (46%) to 2,209 (71%) for Ae. aegypti and from 1,726 (55%) to 2,329 (75%) for Ae. albopictus. Increasing model sensitivity results in more counties classified as suitable, at least for summer survival, from which there are no mosquito records. We anticipate that Ae. aegypti and Ae. albopictus will be found more commonly in counties classified as suitable based on the lower 90% sensitivity threshold compared with the higher 99% threshold. Counties predicted suitable with 90% sensitivity should therefore be a top priority for expanded mosquito surveillance efforts while still keeping in mind that Ae. aegypti and Ae. albopictus may be introduced, via accidental transport of eggs or immatures, and potentially proliferate during the warmest part of the year anywhere within the geographic areas delineated by the 99% sensitivity model. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work

  8. Puente sobre el Maracaibo

    Directory of Open Access Journals (Sweden)

    Equipo Editorial

    2017-05-01

    Full Text Available Para salvar el lago de Maracaibo (Venezuela, el conocido ingeniero italiano Riccaido Morandi ha presentado un proyecto de puente para ferrocarril y carretera, de hormigón pretensado, de tramos independientes, constituyendo un sistema Gerber, que tiene una longitud total de 8.720 m y un vano central de 400 m de luz. Este último tramo, en unión de los dos adyacentes al mismo, constituirán, una vez terminada la obra, un conjunto continuo. El tablero del puente se halla a una altura de 45 m sobre el nivel del agua en la zona del canal, con objeto de permitir la navegación. El tablero, de 20,90 m de anchura total, se ha subdividido en tres partes, dos simétricas respecto al eje, laterales, de 7,20 m de anchura, para la circulación en sentidos opuestos por carretera, una plataforma central, de 5 m de ancho, para el ferrocarril y dos andenes para el paso de peatones y servicios.

  9. Sobre el nombre "Quechua"

    Directory of Open Access Journals (Sweden)

    Rodolfo Cerrón-Palomino

    1985-06-01

    Full Text Available Uno de los primeros problemas con el que a menudo tropiezael estudioso de los idiomas ágrafos, como es el caso de las lenguasaborígenes de América, es el de su denominación (glotónimo.En la medida ,en que, por razones históricas, las noticias sobretales lenguas, y por consiguiente los primeros estudios sobre lasmismas, surgen a partir del contacto con los miembros de la civilización occidental, los nombres de los distintos idiomas, así comode los grupos étnicos que los manejan, son el resultado casi siempre arbitrario cie designaciones fortuitas hechas por viajeros, misiioneros y exploradores, quienes por lo general se basan, para ello, bien en descripciones físicas de los grupo étnicos, en ciertos indios geográficos que constituyen el habitat de los mismos, e incluso en prejuicios que se nutren de un marcado europocentrismo.

  10. In situ fragmentation and rock particle sorting on arid hills

    Science.gov (United States)

    McGrath, Gavan S.; Nie, Zhengyao; Dyskin, Arcady; Byrd, Tia; Jenner, Rowan; Holbeche, Georgina; Hinz, Christoph

    2013-03-01

    Transport processes are often proposed to explain the sorting of rock particles on arid hillslopes, where mean rock particle size often decreases in the downslope direction. Here we show that in situ fragmentation of rock particles can also produce similar patterns. A total of 93,414 rock particles were digitized from 880 photographs of the surface of three mesa hills in the Great Sandy Desert, Australia. Rock particles were characterized by the projected Feret's diameter and circularity. Distance from the duricrust cap was found to be a more robust explanatory variable for diameter than the local hillslope gradient. Mean diameter decreased exponentially downslope, while the fractional area covered by rock particles decreased linearly. Rock particle diameters were distributed lognormally, with both the location and scale parameters decreasing approximately linearly downslope. Rock particle circularity distributions showed little change; only a slight shift in the mode to more circular particles was noted to occur downslope. A dynamic fragmentation model was used to assess whether in situ weathering alone could reproduce the observed downslope fining of diameters. Modeled and observed size distributions agreed well and both displayed a preferential loss of relatively large rock particles and an apparent approach to a terminal size distribution of the rocks downslope. We show this is consistent with a size effect in material strength, where large rocks are more susceptible to fatigue failure under stress than smaller rocks. In situ fragmentation therefore produces qualitatively similar patterns to those that would be expected to arise from selective transport.

  11. ANALYSIS OF A MODEL OF TEAMWORK BY HILL

    Directory of Open Access Journals (Sweden)

    Konstantin Petkovski

    2014-06-01

    Full Text Available The contemporary management of the intellectual capital of an organization, as a major determinant for efficient and effective operation of the organization has access to the teamwork. Teamwork means quality leadership which is necessary for a successful team management. In the theory and the practice are given a number of models for teamwork and team leadership, however, in this case the subject of this paperwork will be the analyzing of the model of team leadership according to Hill. According to this model there are two functions of team leading established: leading functions in the team and leading functions out of the team. In the first part, which refers to the functions of leadership in the team, are set two major categories: team leader’s tasks and the built relationships and atmosphere in the team. In terms of the functions of the leader out of the team, the model focuses on two categories, namely: the functions of leadership out of the team, but within the organization and leading functions of the team outside the organization.

  12. Status of avifauna at Taranga Hill-forest, Gujarat, India

    Directory of Open Access Journals (Sweden)

    C.D. Patel

    2010-02-01

    Full Text Available Taranga is one of the famous pilgrim places of northern Gujarat. It is located (240 00’N & 72046’E at starting point of Aravalli ranges. Climate of this area is semi-arid with irregular rainfall. Variable width line transect method was adapted to study the avifaunal diversity. Taranga Hill-forest has atleast 90 species of birds belonging to 11 orders, 33 families and 68 genera. Passeriformes being the largest family. All common residents appear to be adapted to the prevailing conditions. Red-vented Bulbul and Rock Pigeon were most abundant while Asian Paradise-flycatcher, Crested Bunting and European Roller were rare. White-naped Tit a globally threatened and endemic resident has been found as local migrant, scarce in number, common in occurrence and breeder in the tropical thorn-scrub habitat of THf. Plum-headed Parakeet may be a breeding possible species. In comparison to other places, the avian diversity is observed poor, because Aravallis are not on the migratory route or landing site of migratory birds. In addition, anthropogenic factors, presence of predators and loss of vegetation may be having a telling effect.

  13. Environmental review of the Radium hill mine site, South Australia

    International Nuclear Information System (INIS)

    Lottermoser, B.G.; Ashley, P.M.

    2005-01-01

    The Radium Hill uranium deposit, in semi-arid eastern South Australia, was discovered in 1906 and mined for radium between 1906 and 1931 and for uranium between 1954 and 1961 (production of 969,300 t of davidite ore averaging 0.12% U 3 O 8 ). Rehabilitation was limited to removal of mine facilities, sealing of underground workings and capping of selected waste repositories. In 2002, gamma-ray data, plus tailings, uncrushed and crushed waste rock, stream sediment, topsoil and vegetation samples were collected to assist in the examination of the current environmental status of the mine site. The preliminary data indicate that capping of tailings storage facilities did not ensure the long-term containment of the low-level radioactive wastes due to the erosion of sides of the impoundments. Moreover, active wind erosion of waste fines from various, physically unstable waste repositories causes increasing radiochemical (up to 0.94 μSv/h) and geochemical (Ce, La, Sc, Th, U, V, Y) impacts on local soils and sediments. However, measured radiation levels of soils and sediments are at or below Australian Radiation Protection Standards (20 mSv/a averaged over five consecutive years). Additional capping and landform design of the crushed waste and tailings repositories are required in order to minimise erosion and impacts on surrounding soils and sediments. (orig.)

  14. Efficient utilization of rain water in hill agriculture

    International Nuclear Information System (INIS)

    Singh, S.R.; Singh, S.S.; Khan, A.R.

    2002-05-01

    Hill areas generally receive rainfall of around or more than 1150 mm. More than 2500 mm rainfall is received in about 30.2 million ha hilly area of the country. Besides many other factors high rainfall and heavy runoff are mainly responsible for low productivity. Because of sloppy characters and shallow soil depth, major fraction of rainwater is lost as runoff. Invariably evaporation exceeds the moisture stored and thereby depletes soils of their moisture reserve when crops are to be sown. Too much water at one time and too little in another during the same year causes wide instability in the production and productivity (Gupta et al., 2000). In high rainfall/hilly areas small and scattered land holdings exclusively rain dependent subsistence type of agriculture, low irrigated area of eastern Himalaya (northeastern region of India) further aggravate the problem. Harvesting of runoff at micro level for storage and recycling, control of erosion and moisture conservation are necessary and possible measures for better crop production. (author)

  15. Hill Cipher and Least Significant Bit for Image Messaging Security

    Directory of Open Access Journals (Sweden)

    Muhammad Husnul Arif

    2016-02-01

    Full Text Available Exchange of information through cyberspace has many benefits as an example fast estimated time, unlimited physical distance and space limits, etc. But in these activities can also pose a security risk for confidential information. It is necessary for the safety that can be used to protect data transmitted through the Internet. Encryption algorithm that used to encrypt message to be sent (plaintext into messages that have been randomized (ciphertext is cryptography and steganography algorithms. In application of cryptographic techniques that will be used is Hill Cipher. The technique is combined with steganography techniques Least Significant Bit. The result of merging techniques can maintain the confidentiality of messages because people who do not know the secret key used will be difficult to get the message contained in the stego-image and the image that has been inserted can not be used as a cover image. Message successfully inserted and extracted back on all samples with a good image formats * .bmp, * .png , * .jpg at a resolution of 512 x 512 pixels , 256 x 256 pixels. MSE and PSNR results are not influenced file format or file size, but influenced by dimensions of image. The larger dimensions of the image, then the smaller MSE that means error of image gets smaller.

  16. Performance of Garden Pea Genotypes in Eastern Hills of Nepal

    Directory of Open Access Journals (Sweden)

    Krishna Poudel

    2017-05-01

    Full Text Available Garden pea (Pisum sativum L is an important winter legume used as fresh vegetables and other drier food products. Despite of its importance as cash crop in many parts of Nepal, much study on various aspects for enhancing production and productivity has yet to be done. Therefore, to evaluate the production performance different genotypes of garden pea in eastern hills agro-ecological conditions present experiments were carried out consecutively for two years (2015 and 2016 at Agricultural Research Station, Pakhribas. The experiment comprised of 11 different genotypes of garden pea including a check variety Arkel. The production performance was evaluated in a completely randomized block design with three replications. The seeds were sown at 50 × 10 cm spacing during first week of October for two years. The result showed that DGP-05 genotype had earliest 104 days after sowing. The DGP-08 genotype showed 13 which were the maximum numbers of pods per plant (13, while DGP-01 showed 8 numbers of seeds as the maximum per pod. The DGP-03 genotype had the longest pod of 9.78 cm among others. The highest fresh pod yield of 18.14 t/ha was achieved from genotype DGP-09 followed by Arkel with (16.32 t/ha.

  17. The Myres Hill remote sensing intercomparison study: preliminary results

    Energy Technology Data Exchange (ETDEWEB)

    Clive, P J M; Chindurza, I [SgurrEnergy Ltd, 79 Coplaw Street, Glasgow G42 7JG, Scotland (United Kingdom); Ravey, I; Bass, J [RES Group Ltd, James Blyth House, 7000 Academy Park, Glasgow, Scotland (United Kingdom); Boyle, R J; Jones, P [TUV NEL Ltd, East Kilbride, Glasgow G75 0QF, Scotland (United Kingdom); Lang, S J [Sustainable Energy Research Group, University College Cork (Ireland); Bradley, S [Mighty River Power, Level 14, 23-29 Albert Street, Auckland (New Zealand); Hay, L [Garrad Hassan and Partners Ltd, 2064 Maryhill Road, Glasgow G20 0AB, Scotland (United Kingdom); Oldroyd, A [Oldbaum Services Ltd, Schoolhouse, Brig o' Turk, Callander, Scotland (United Kingdom); Stickland, M [University of Strathclyde, 16 Richmond Street, Glasgow G1 1XQ, Scotland (United Kingdom)], E-mail: peter.clive@sgurrenergy.com

    2008-05-01

    Two remote sensing techniques (SODAR and LIDAR) have been developed for measuring wind speed and turbulence from ground level up to altitudes of 300 m or higher. Although originally developed in the defence sector, these techniques are now generating considerable interest in the renewable energy and meteorological sectors. Despite the benefits of these instruments they are not yet generally accepted for due diligence measurements by wind energy developers and financial institutions. There is a requirement for a series of independent assessments of these new metrology techniques, comparing their measurements with the approved cup-type anemometer readings. This is being addressed at TUV NEL's Myres Hill wind turbine test site in a measurement programme supported by the DIUS National Measurement Systems Measurement for Innovators scheme and a consortium of 21 industrial collaborators. Data from SODAR and LIDAR systems are being compared with results from cup-type anemometers mounted at different heights on an 80m meteorological mast. An ultrasonic sensor is also mounted on the mast. The objective of the test programme is to assess the effectiveness of SODAR and LIDAR wind speed measurement techniques under different operating regimes and atmospheric conditions. Results from the measurements will provide definitive data on the performance of the remote wind speed sensing techniques under test on complex terrain typical of many wind farm sites. Preliminary measurements based on data acquired during the initial measurement campaign are presented.

  18. Use of health services in Hill villages in central Nepal.

    Science.gov (United States)

    Niraula, B B

    1994-10-01

    This paper reports the use and non-use of health care facilities in the Hill villages in central Nepal. The health behaviour model (HBM) is applied to test the significance of socioeconomic variables on the use of the modern health care system. The study finds that all three characteristics of the HBM model, predisposing, enabling and need, are significantly related to use and non-use of the modern health care system. The analysis shows that number of living children, respondent's education, nearness to the road and service centre, value of land, knowledge about health workers and experience of child loss are some of the variables that are positively and significantly related to the use of modern health care. Age of the respondents and household size were found to be negatively associated with health-care use. Contrary to expectation, caste is unimportant. Making use of the qualitative data, this paper argues that the health care system is unnecessarily bureaucratic and patriarchal, which favours the socio-economically well-off.

  19. Gender, professionalization, and the child in the Progressive Era: Patty Smith Hill, 1868-1946.

    Science.gov (United States)

    Allen, Ann Taylor

    2011-01-01

    This article discusses the career of Patty Smith Hill, a major figure in the American kindergarten movement, in the context of the Progressive Era in American history. Hill, an educator and child-welfare activist, became known both as a reformer of early-childhood education and as an advocate of the inclusion of the kindergarten, originally a private institution, in public-school systems. The article acknowledges this as one of the most significant achievements of the woman-led reform movements of the Progressive Era, but at the same time notes that it involved a substantial transfer of power from the women who had originally developed the kindergarten to the male principals and superintendants who now supervised kindergarten teachers, often without much understanding of their distinctive methods and aims. As a professor at Columbia Teachers College, Hill also exercised an international influence. Hill's career exemplifies broader patterns of women's professionalization during the late-nineteenth and early-twentieth centuries.

  20. NPP Grassland: Beacon Hill, U.K., 1972-1993, R1

    Data.gov (United States)

    National Aeronautics and Space Administration — This data set contains two ASCII text files, one providing productivity measurements for a chalk grassland on Beacon Hill, West Sussex, U.K. (50.92 N, -0.85 W) and...

  1. Paul Hill d/b/a Alternative Energy Windows and Siding

    Science.gov (United States)

    Paul Hill d/b/a Alternative Energy Windows and Siding (the Company) is located in Concord, New Hampshire. The settlement involves renovation activities conducted at property constructed prior to 1978, located in Concord, New Hampshire.

  2. 76 FR 48120 - Black Hills National Forest, Custer, SD-Mountain Pine Beetle Response Project

    Science.gov (United States)

    2011-08-08

    ... species on lands of all ownerships in the Black Hills is ponderosa pine (Pinus ponderosae). Since 1997 the... rated as having high wildfire hazard. Since 1980, due to several factors including drought the Forest...

  3. 77 FR 10717 - Black Hills National Forest, Custer, South Dakota-Mountain Pine Beetle Response Project

    Science.gov (United States)

    2012-02-23

    .... The predominant tree species on lands of all ownerships in the Black Hills is ponderosa pine (Pinus... drought the Forest has seen a dramatic increase in acreage burned by wildfires. In that period over 250...

  4. Systematic studies of Bornean Zingiberaceae V. Zingiberoideae of Lambir Hills, Sarawak

    NARCIS (Netherlands)

    Sakai, S.; Nagamasu, H.

    2006-01-01

    This paper reports the subfamily Zingiberoideae (Zingiberaceae) of Lambir Hills National Park, Sarawak, Malaysia. Twelve species representing Boesenbergia, Globba, and Zingiber are recorded. Systematic and ecological notes are provided, and the key to the Bornean species of Boesenbergia is updated.

  5. Mida teha, et lapsed koolist ei puuduks? / Triin Tomingas, Hille Hinsberg, Sirje Ess...[jt

    Index Scriptorium Estoniae

    2011-01-01

    Küsimusele vastavad lapsevanemad Triin Tomingas ja Hille Hinsberg, Saaremaa ühisgümnaasiumi algklasside õppealajuhataja Sirje Ess, Tallinna Pelgulinna gümnaasiumi direktor Tõnu Piibur, MTÜ Ellu psühholoog-koolitaja Tiia Lister

  6. On the numerical treatment of the Griffin-Hill-Eheeler equation

    International Nuclear Information System (INIS)

    Galleti, D.; Toledo Piza, A.F.R.

    The precision attainable in the numerical treatment of the Griffin-Hill-Wheeler equation is studied in a solvable model. Trucation errors related to the generator coordinate kinematics are exhibited and briefly discussed [pt

  7. CH2M Hill Hanford Group Inc (CHG) Information Resource Management (IRM) Strategic Plan

    Energy Technology Data Exchange (ETDEWEB)

    NELSON, R.L.

    2000-05-08

    The CH2M Hill Hanford Group, Inc., Information Resource Management Strategic Plan is the top-level planning document for applying information and information resource management to achieve the CHG mission for the management of the River Protection Project

  8. Preliminary report on diatoms from the deep lake terraces, Vestfold Hills, Antarctica

    Digital Repository Service at National Institute of Oceanography (India)

    Setty, M.G.A.P.; Kellogg, D.E.; Kellogg, T.B.

    with fragments of pyroxenite, quartzite, permatite, gneiss, and glacial debris. Halite and mirabilite crystals are common. A general study of foraminifera of the lake sediments of the Vestfold Hills was made and the results are presented here...

  9. Turbulence Models: Data from Other Experiments: FAITH Hill 3-D Separated Flow

    Data.gov (United States)

    National Aeronautics and Space Administration — Exp: FAITH Hill 3-D Separated Flow. This web page provides data from experiments that may be useful for the validation of turbulence models. This resource is...

  10. CH2M Hill Hanford Group Inc (CHG) Information Resource Management (IRM) Strategic Plan

    International Nuclear Information System (INIS)

    NELSON, R.L.

    2000-01-01

    The CH2M Hill Hanford Group, Inc., Information Resource Management Strategic Plan is the top-level planning document for applying information and information resource management to achieve the CHG mission for the management of the River Protection Project

  11. The West Beverly Hills Lineament and Beverly Hills High School: Ethical Issues in Geo-Hazard Communication

    Science.gov (United States)

    Gath, Eldon; Gonzalez, Tania; Roe, Joe; Buchiarelli, Philip; Kenny, Miles

    2014-05-01

    Results of geotechnical studies for the Westside Subway were disclosed in a public hearing on Oct. 19, 2011, showing new "active faults" of the Santa Monica fault and the West Beverly Hills Lineament (WBHL), identified as a northern extension of the Newport-Inglewood fault. Presentations made spoke of the danger posed by these faults, the possibility of killing people, and how it was good news that these faults had been discovered now instead of later. The presentations were live and are now memorialized as YouTube videos, (http://www.youtube.com/watch?v=Omx2BTIpzAk and others). No faults had been physically exposed or observed by the study; the faults were all interpreted from cone penetrometer probes, supplemented by core borings and geophysical transects. Several of the WBHL faults traversed buildings of the Beverly Hills High School (BHHS), triggering the school district to geologically map and characterize these faults for future planning efforts, and to quantify risk to the students in the 1920's high school building. 5 exploratory trenches were excavated within the high school property, 12 cone penetrometers were pushed, and 26-cored borings were drilled. Geologic logging of the trenches and borings and interpretation of the CPT data failed to confirm the presence of the mapped WBHL faults, instead showing an unfaulted, 3° NE dipping sequence of mid-Pleistocene alluvial fan deposits conformably overlying an ~1 Ma marine sand. Using 14C, OSL, and soil pedology for stratigraphic dating, the BHHS site was cleared from fault rupture hazards and the WBHL was shown to be an erosional margin of Benedict Canyon, partially buttressed by 40-200 ka alluvial deposits from Benedict Wash. The consequence of the Westside Subway's active fault maps has been the unexpected expenditure of millions of dollars for emergency fault investigations at BHHS and several other private properties within a densely developed urban highrise environment. None of these studies have found

  12. Optimal barrier zones for stopping the invasion of Aedes aegypti mosquitoes via transgenic or sterile insect techniques

    KAUST Repository

    Lee, S. Seirin; Baker, Ruth E.; Gaffney, Eamonn A.; White, Steven M.

    2013-01-01

    (Release of Insects carrying a Dominant Lethal), for controlling invasion of the mosquito Aedes aegypti using a spatial stage-structured mathematical model. In particular, we explore the use of a barrier zone of sterile/transgenic insects to prevent

  13. Developing Exon-Primed Intron-Crossing (EPIC) markers for population genetic studies in three Aedes disease vectors.

    Science.gov (United States)

    White, Vanessa Linley; Endersby, Nancy Margaret; Chan, Janice; Hoffmann, Ary Anthony; Weeks, Andrew Raymond

    2015-03-01

    Aedes aegypti, Aedes notoscriptus, and Aedes albopictus are important vectors of many arboviruses implicated in human disease such as dengue fever. Genetic markers applied across vector species can provide important information on population structure, gene flow, insecticide resistance, and taxonomy, however, robust microsatellite markers have proven difficult to develop in these species and mosquitoes generally. Here we consider the utility and transferability of 15 Ribosome protein (Rp) Exon-Primed Intron-Crossing (EPIC) markers for population genetic studies in these 3 Aedes species. Rp EPIC markers designed for Ae. aegypti also successfully amplified populations of the sister species, Ae. albopictus, as well as the distantly related species, Ae. notoscriptus. High SNP and good indel diversity in sequenced alleles plus support for amplification of the same regions across populations and species were additional benefits of these markers. These findings point to the general value of EPIC markers in mosquito population studies. © 2014 Institute of Zoology, Chinese Academy of Sciences.

  14. Aedes aegypti (Diptera: Culicidae) in Mauritania: First Report on the Presence of the Arbovirus Mosquito Vector in Nouakchott.

    Science.gov (United States)

    Mint Lekweiry, Khadijetou; Ould Ahmedou Salem, Mohamed Salem; Ould Brahim, Khyarhoum; Ould Lemrabott, Mohamed Aly; Brengues, Cécile; Faye, Ousmane; Simard, Frédéric; Ould Mohamed Salem Boukhary, Ali

    2015-07-01

    Aedes aegypti L. (Diptera: Culicidae) is a major vector of yellow fever, dengue, and chikungunya viruses throughout tropical and subtropical areas of the world. Although the southernmost part of Mauritania along the Senegal river has long been recognized at risk of yellow fever transmission, Aedes spp. mosquitoes had never been reported northwards in Mauritania. Here, we report the first observation of Aedes aegypti aegypti (L.) and Aedes (Ochlerotatus) caspius (Pallas, 1771) in the capital city, Nouakchott. We describe the development sites in which larvae of the two species were found, drawing attention to the risk for emergence of arbovirus transmission in the city. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  15. Immature Aedes mosquitoes colonize Culex quinquefasciatus breeding sites in neighborhoods in the municipality of Olinda, State of Pernambuco

    Directory of Open Access Journals (Sweden)

    Suzane Alves dos Santos

    2014-12-01

    Full Text Available Introduction The present study shows the colonization of Aedes mosquitoes in breeding sites specific for Culex quinquefasciatus in neighborhoods in the municipality of Olinda. Methods Samples were collected between May 2011 and June 2012 from breeding sites positive for Cx. quinquefasciatus by using a ladle and manual suction pump. Results Aedes aegypti (0.12%, Aedes albopictus (0.03%, and Cx. quinquefasciatus (99.8% were found across the breeding sites. Conclusions The presence of Aedes ssp. in several Cx. quinquefasciatus breeding sites with a heavy load of organic material demonstrates the need to review the concepts and methods used for treatment, as the use of specific larvicide for breeding sites of Culex.

  16. Mass, charge, and energy separation by selective acceleration with a traveling potential hill

    Science.gov (United States)

    Tung, L. Schwager; Barr, W. L.; Lowder, R. S.; Post, R. F.

    1996-10-01

    A traveling electric potential hill has been used to generate an ion beam with an energy distribution that is mass dependent from a monoenergetic ion beam of mixed masses. This effect can be utilized as a novel method for mass separation applied to identification or enrichment of ions (e.g., of elements, isotopes, or molecules). This theory for mass-selective acceleration is presented here and is shown to be confirmed by experiment and by a time-dependent particle-in-cell computer simulation. Results show that monoenergetic ions with the particular mass of choice are accelerated by controlling the hill potential and the hill velocity. The hill velocity is typically 20%-30% faster than the ions to be accelerated. The ability of the hill to pickup a particular mass uses the fact that small kinetic energy differences in the lab frame appear much larger in the moving hill frame. Ions will gain energy from the approaching hill if their relative energy in the moving hill frame is less than the peak potential of the hill. The final energy of these accelerated ions can be several times the source energy, which facilitates energy filtering for mass purification or identification. If the hill potential is chosen to accelerate multiple masses, the heaviest mass will have the greatest final energy. Hence, choosing the appropriate hill potential and collector retarding voltage will isolate ions with the lightest, heaviest, or intermediate mass. In the experimental device, called a Solitron, purified 20Ne and 22Ne are extracted from a ribbon beam of neon that is originally composed of 20Ne:22Ne in the natural ratio of 91:9. The isotopic content of the processed beam is determined by measuring the energy distribution of the detected current. These results agree with the theory. In addition to mass selectivity, our theory can also be applied to the filtration of an ion beam according to charge state or energy. Because of this variety of properties, the Solitron is envisioned to

  17. Distance-Based Functional Diversity Measures and Their Decomposition: A Framework Based on Hill Numbers

    Science.gov (United States)

    Chiu, Chun-Huo; Chao, Anne

    2014-01-01

    Hill numbers (or the “effective number of species”) are increasingly used to characterize species diversity of an assemblage. This work extends Hill numbers to incorporate species pairwise functional distances calculated from species traits. We derive a parametric class of functional Hill numbers, which quantify “the effective number of equally abundant and (functionally) equally distinct species” in an assemblage. We also propose a class of mean functional diversity (per species), which quantifies the effective sum of functional distances between a fixed species to all other species. The product of the functional Hill number and the mean functional diversity thus quantifies the (total) functional diversity, i.e., the effective total distance between species of the assemblage. The three measures (functional Hill numbers, mean functional diversity and total functional diversity) quantify different aspects of species trait space, and all are based on species abundance and species pairwise functional distances. When all species are equally distinct, our functional Hill numbers reduce to ordinary Hill numbers. When species abundances are not considered or species are equally abundant, our total functional diversity reduces to the sum of all pairwise distances between species of an assemblage. The functional Hill numbers and the mean functional diversity both satisfy a replication principle, implying the total functional diversity satisfies a quadratic replication principle. When there are multiple assemblages defined by the investigator, each of the three measures of the pooled assemblage (gamma) can be multiplicatively decomposed into alpha and beta components, and the two components are independent. The resulting beta component measures pure functional differentiation among assemblages and can be further transformed to obtain several classes of normalized functional similarity (or differentiation) measures, including N-assemblage functional generalizations of

  18. Investigation of periodic systems by means of the generalized Hill method

    International Nuclear Information System (INIS)

    Baitin, A.V.; Ivanov, A.A.

    1994-01-01

    We propose the new method of investigation of infinite periodic determination which is a generalized Hill method. This method has been used for finding of the characteristic value for the Hill equation. finding the band structure of the one-dimensional periodic and obtaining of the dispersion equation for the electromagnetic wave propagation in the waveguide by plasma arbitrary periodic density modulation by plasma arbitrary periodic density modulation

  19. Orno-Cotino-Quercetum Pubescentis Ass. nova prov. on the slopes of Titel hill (Serbia

    Directory of Open Access Journals (Sweden)

    Butorac Branislava

    2008-01-01

    Full Text Available Studied by many botanists from the floristic and vegetation-geographical aspects over the last hundred years, the herbaceous plant cover in dominant on Titel Hill. The first data on plant communities were registered in 1983, when a significant contribution to the study of vegetation of steppe character was made. At that time, presence of shrubby remnants of forest vegetation on Titel Hill was registered. Since 1983, fragments of typical xerothermic woods of pubescent oak have been registered.

  20. Review of Barrow Hill: Curse of the Ancient Circle PC Game

    OpenAIRE

    Michael Charno

    2007-01-01

    Archaeological video games are few and far between. Internet Archaeology has reviewed a number of archaeologically-based computer applications, but none that in my opinion would fall under the video game category. Barrow Hill: Curse of the ancient circle from Shadow Tor Studios, is a video game…with archaeology in it. It would be a stretch to claim that Barrow Hill is actually an archaeological video game, but it does have an occasionally solid archaeological foundation and back story.

  1. Solar heating and hot water system installed at Cherry Hill, New Jersey

    Science.gov (United States)

    1979-01-01

    The solar heating and hot water system installed in existing buildings at the Cherry Hill Inn in Cherry Hill, New Jersey is described in detail. The system is expected to furnish 31.5% of the overall heating load and 29.8% of the hot water load. The collectors are liquid evacuated tube type. The storage system is an above ground insulated steel water tank with a capacity of 7,500 gallons.

  2. Distance-based functional diversity measures and their decomposition: a framework based on Hill numbers.

    Directory of Open Access Journals (Sweden)

    Chun-Huo Chiu

    Full Text Available Hill numbers (or the "effective number of species" are increasingly used to characterize species diversity of an assemblage. This work extends Hill numbers to incorporate species pairwise functional distances calculated from species traits. We derive a parametric class of functional Hill numbers, which quantify "the effective number of equally abundant and (functionally equally distinct species" in an assemblage. We also propose a class of mean functional diversity (per species, which quantifies the effective sum of functional distances between a fixed species to all other species. The product of the functional Hill number and the mean functional diversity thus quantifies the (total functional diversity, i.e., the effective total distance between species of the assemblage. The three measures (functional Hill numbers, mean functional diversity and total functional diversity quantify different aspects of species trait space, and all are based on species abundance and species pairwise functional distances. When all species are equally distinct, our functional Hill numbers reduce to ordinary Hill numbers. When species abundances are not considered or species are equally abundant, our total functional diversity reduces to the sum of all pairwise distances between species of an assemblage. The functional Hill numbers and the mean functional diversity both satisfy a replication principle, implying the total functional diversity satisfies a quadratic replication principle. When there are multiple assemblages defined by the investigator, each of the three measures of the pooled assemblage (gamma can be multiplicatively decomposed into alpha and beta components, and the two components are independent. The resulting beta component measures pure functional differentiation among assemblages and can be further transformed to obtain several classes of normalized functional similarity (or differentiation measures, including N-assemblage functional

  3. Medical Entomology Studies - XI. The Subgenus Stegomyia of Aedes in the Oriental Region with Keys to the Species (Diptera: Culicidae)

    Science.gov (United States)

    1979-01-01

    Dengue l-4 viruses Saigon area, Vietnam Dengue 3 virus Rangoon, Burma Zika virus Bentong, Malaysia 2 5 isolations Smith et al. from 88 pools...and A. RUDNICK. 1969. Isolation of Zika virus from Aedes aegypti mosquitoes in Malaysia. Am. J. Trop. Med. Hyg. 18: 411-5. MATSUO, K., YOSHIDA, Y...number of virus diseases. It is one of the most dominant subgenera of the genus Aedes Meigen in the Oriental region, as indicated by the number of

  4. The origin of Mauna Loa's Nīnole Hills: Evidence of rift zone reorganization

    Science.gov (United States)

    Zurek, Jeffrey; Williams-Jones, Glyn; Trusdell, Frank A.; Martin, Simon

    2015-01-01

    In order to identify the origin of Mauna Loa volcano's Nīnole Hills, Bouguer gravity was used to delineate density contrasts within the edifice. Our survey identified two residual anomalies beneath the Southwest Rift Zone (SWRZ) and the Nīnole Hills. The Nīnole Hills anomaly is elongated, striking northeast, and in inversions both anomalies merge at approximately −7 km above sea level. The positive anomaly, modeled as a rock volume of ~1200 km3 beneath the Nīnole Hills, is associated with old eruptive vents. Based on the geologic and geophysical data, we propose that the gravity anomaly under the Nīnole Hills records an early SWRZ orientation, now abandoned due to geologically rapid rift-zone reorganization. Catastrophic submarine landslides from Mauna Loa's western flank are the most likely cause for the concurrent abandonment of the Nīnole Hills section of the SWRZ. Rift zone reorganization induced by mass wasting is likely more common than currently recognized.

  5. Experimental investigation of flow over two-dimensional multiple hill models.

    Science.gov (United States)

    Li, Qing'an; Maeda, Takao; Kamada, Yasunari; Yamada, Keisuke

    2017-12-31

    The aim of this study is to investigate the flow field characteristics in ABL (Atmospheric Boundary Layer) flow over multiple hills and valleys in two-dimensional models under neutral conditions. Active turbulence grids and boundary layer generation frame were used to simulate the natural winds in wind tunnel experiments. As a result, the mean wind velocity, the velocity vector diagram and turbulence intensity around the hills were investigated by using a PIV (Particle Image Velocimetry) system. From the measurement results, it was known that the average velocity was increased along the upstream slope of upside hill, and then separated at the top of the hills, the acceleration region of U/U ref >1 was generated at the downstream of the hill. Meanwhile, a large clockwise circulation flow was generated between the two hill models. Moreover, the turbulence intensity showed small value in the circulation flow regions. Compared to 1H model, the turbulence intensity in the mainstream direction showed larger value than that in the vertical direction. This paper provided a better understanding of the wind energy distribution on the terrain for proper selection of suitable sites for installing wind farms in the ABL. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Exploring New Thermal Fog and Ultra-Low Volume Technologies to Improve Indoor Control of the Dengue Vector, Aedes aegypti (Diptera: Culicidae)

    Science.gov (United States)

    2014-07-01

    Dengue Vector, Aedes aegypti (Diptera: Culicidae) JAMES F. HARWOOD,1,2 MUHAMMAD FAROOQ,1 ALEC G. RICHARDSON,1 CARL W. DOUD,1 JOHN L. PUTNAM,3 DANIEL E...vector, Aedes aegypti (L.), inside human habitations must be performed quickly and efÞciently to reduce the risk of transmission during dengue...immediate knockdown of vector populations that may lower the risk of infection and allow other suppression strategies to be implemented. KEY WORDS Aedes

  7. Composition of the Essential Oil of Pink Chablis Bluebeard (Caryopteris x clandonensis ’Durio’) and Its Biological Activity against the Yellow Fever Mosquito Aedes aegypti

    Science.gov (United States)

    2016-04-07

    oil of Pink Chablis™ bluebeard (Caryopteris ×clandonensis ’Durio’) and its biological activity against the yellow fever mosquito Aedes aegypti ARTICLE...bluebeard (Caryopteris ×clandonensis ’Durio’) and its biological activity against the yellow fever mosquito Aedes aegypti Eugene K. Blythe1...mosquito [ Aedes aegypti (L.) (Diptera: Culicidae)]. Essential oil from the aerial parts of this mildly aromatic ornamental species was extracted by water

  8. Impact of Diurnal Temperature Fluctuations during Larval Development on Adult Life History Traits and Insecticide Susceptibility in Two Vectors; Anopheles gambiae and Aedes aegypti

    Science.gov (United States)

    2014-04-30

    ANOPHELES GAMBIAE AND AEDES AEGYPTI. by Jeffrey W. Clark Dissertation submitted to the Faculty of the Department of Preventive Medicine and...Vectors; Anopheles gambiae and Aedes aegypti." Name of Candidate: Jeffrey Clark Doctor of Philosophy Degree April 30, 2014 DISSERTATION AND ABSTRACT...for the many fruitful discussions and the standing offer to help whenever I needed it; and to Joe Wagman, for providing needed Aedes aegypti eggs from

  9. Daya Repelan dan Uji Iritasi Formula Lotion Ekstrak Etanol Daun Sirih (Piper Betle Linn) dengan Variasi Basis Lanolin terhadap Nyamuk Aedes Aegypti

    OpenAIRE

    Fitriana, Apri Yudis; Wahyuningrum, Retno; Sudarso, Sudarso

    2012-01-01

    Demam berdarah dengue (DBD) yang ditularkan melalui gigitan nyamuk Aedes aegypti dan Aedes albopictus betina masih menjadi masalah kesehatan khususnya di Indonesia. Sediaan repelan biasa digunakan untuk menghindari gangguan atau gigitan nyamuk Aedes aegypti. Namun sediaan repelan mengandung Diethyl toluamide (DEET) yang dalam penggunaannya dapat menyebabkan eritema (kemerahan pada kulit) dan iritasi. Penelitian ini bertujuan untuk menentukan aktivitas daya repelan lotion ekstrak daun sirih de...

  10. Algunos apuntes sobre comunidad

    Directory of Open Access Journals (Sweden)

    Clara Pérez Cárdenas

    2000-08-01

    Full Text Available Comunidad es, entre los temas de estudio del curriculum de los residentes en Medicina General Integral, uno de aquellos en las que mayores dificultades encontramos para poderlo impartir, pues la bibliografía sobre el tema está un tanto dispersa. Es un aspecto que los propios residentes no evalúan con justeza; sin embargo es de sumo interés que como profesionales de la salud no obviemos que nuestro trabajo de promoción, prevención y tratamiento, debe darse a nivel de individuo, pero también en las familias y en la comunidad como un todo, y por lo tanto debamos incorporar, al menos, nociones de lo que es una comunidad y qué es participación comunitaria para tener idea de cómo fomentarla, única vía para que nuestras acciones de salud sean más efectivasCommunity is one of the most difficult subjects to be taught among those included in the curriculum of General Comprehensive Medicine residents, since bibliography on this topic is a bit dispersed. This aspect, which is not properly evaluated by residents, is very important for health professionals to do our work of promotion, prevention and treatment not only at the level of the individual, but also at the level of the family and of the community as a whole. Therefore, we need to have some notions about community and community participation to be able to promote them, as the only way to make our health actions more effective

  11. Human IgG Antibody Response to Aedes Nterm-34kDa Salivary Peptide, an Epidemiological Tool to Assess Vector Control in Chikungunya and Dengue Transmission Area.

    Science.gov (United States)

    Elanga Ndille, Emmanuel; Doucoure, Souleymane; Poinsignon, Anne; Mouchet, François; Cornelie, Sylvie; D'Ortenzio, Eric; DeHecq, Jean Sébastien; Remoue, Franck

    2016-12-01

    Arboviral diseases are an important public health concerns. Vector control remains the sole strategy to fight against these diseases. Because of the important limits of methods currently used to assess human exposure to Aedes mosquito bites, much effort is being devoted to develop new indicators. Recent studies have reported that human antibody (Ab) responses to Aedes aegypti Nterm-34kDa salivary peptide represent a promising biomarker tool to evaluate the human-Aedes contact. The present study aims investigate whether such biomarker could be used for assessing the efficacy of vector control against Aedes. Specific human IgG response to the Nterm-34kDa peptide was assessed from 102 individuals living in urban area of Saint-Denis at La Reunion Island, Indian Ocean, before and after the implementation of vector control against Aedes mosquitoes. IgG response decreased after 2 weeks (P Aedes mosquito density, as estimated by entomological parameters and closely correlated to vector control implementation and was not associated with the use of individual protection, daily commuting outside of the house, sex and age. Our findings indicate a probable short-term decrease of human exposure to Aedes bites just after vector control implementation. Results provided in the present study indicate that IgG Ab response to Aedes aegypti Nterm-34kDa salivary peptide could be a relevant short-time indicator for evaluating the efficacy of vector control interventions against Aedes species.

  12. HABITABILITY OF EXOMOONS AT THE HILL OR TIDAL LOCKING RADIUS

    Energy Technology Data Exchange (ETDEWEB)

    Hinkel, Natalie R.; Kane, Stephen R., E-mail: natalie.hinkel@gmail.com [NASA Exoplanet Science Institute, Caltech, MS 100-22, 770 South Wilson Avenue, Pasadena, CA 91125 (United States)

    2013-09-01

    Moons orbiting extrasolar planets are the next class of object to be observed and characterized for possible habitability. Like the host-planets to their host-star, exomoons have a limiting radius at which they may be gravitationally bound, or the Hill radius. In addition, they also have a distance at which they will become tidally locked and therefore in synchronous rotation with the planet. We have examined the flux phase profile of a simulated, hypothetical moon orbiting at a distant radius around the confirmed exoplanets {mu} Ara b, HD 28185 b, BD +14 4559 b, and HD 73534 b. The irradiated flux on a moon at its furthest, stable distance from the planet achieves its largest flux gradient, which places a limit on the flux ranges expected for subsequent (observed) moons closer in orbit to the planet. We have also analyzed the effect of planetary eccentricity on the flux on the moon, examining planets that traverse the habitable zone either fully or partially during their orbit. Looking solely at the stellar contributions, we find that moons around planets that are totally within the habitable zone experience thermal equilibrium temperatures above the runaway greenhouse limit, requiring a small heat redistribution efficiency. In contrast, exomoons orbiting planets that only spend a fraction of their time within the habitable zone require a heat redistribution efficiency near 100% in order to achieve temperatures suitable for habitability. This means that a planet does not need to spend its entire orbit within the habitable zone in order for the exomoon to be habitable. Because the applied systems comprise giant planets around bright stars, we believe that the transit detection method is most likely to yield an exomoon discovery.

  13. HABITABILITY OF EXOMOONS AT THE HILL OR TIDAL LOCKING RADIUS

    International Nuclear Information System (INIS)

    Hinkel, Natalie R.; Kane, Stephen R.

    2013-01-01

    Moons orbiting extrasolar planets are the next class of object to be observed and characterized for possible habitability. Like the host-planets to their host-star, exomoons have a limiting radius at which they may be gravitationally bound, or the Hill radius. In addition, they also have a distance at which they will become tidally locked and therefore in synchronous rotation with the planet. We have examined the flux phase profile of a simulated, hypothetical moon orbiting at a distant radius around the confirmed exoplanets μ Ara b, HD 28185 b, BD +14 4559 b, and HD 73534 b. The irradiated flux on a moon at its furthest, stable distance from the planet achieves its largest flux gradient, which places a limit on the flux ranges expected for subsequent (observed) moons closer in orbit to the planet. We have also analyzed the effect of planetary eccentricity on the flux on the moon, examining planets that traverse the habitable zone either fully or partially during their orbit. Looking solely at the stellar contributions, we find that moons around planets that are totally within the habitable zone experience thermal equilibrium temperatures above the runaway greenhouse limit, requiring a small heat redistribution efficiency. In contrast, exomoons orbiting planets that only spend a fraction of their time within the habitable zone require a heat redistribution efficiency near 100% in order to achieve temperatures suitable for habitability. This means that a planet does not need to spend its entire orbit within the habitable zone in order for the exomoon to be habitable. Because the applied systems comprise giant planets around bright stars, we believe that the transit detection method is most likely to yield an exomoon discovery

  14. Fracture Patterns within the Shale Hills Critical Zone Observatory

    Science.gov (United States)

    Singha, K.; White, T.; Perron, J.; Chattopadhyay, P. B.; Duffy, C.

    2012-12-01

    Rock fractures are known to exist within the deep Critical Zone and are expected to influence groundwater flow, but there are limited data on their orientation and spatial arrangement and no general framework for systematically predicting their effects. Here, we explore fracture patterns within the Susquehanna-Shale Hills Critical Zone Observatory, and consider how they may be influenced by weathering, rock structure, and stress via field observations of variable fracture orientation within the site, with implications for the spatial variability of structural control on hydrologic processes. Based on field observations from 16-m deep boreholes and surface outcrop, we suggest that the appropriate structural model for the watershed is steeply dipping strata with meter- to decimeter-scale folds superimposed, including a superimposed fold at the mouth of the watershed that creates a short fold limb with gently dipping strata. These settings would produce an anisotropy in the hydraulic conductivity and perhaps also flow, especially within the context of the imposed stress field. Recently conducted 2-D numerical stress modeling indicates that the proxy for shear fracture declines more rapidly with depth beneath valleys than beneath ridgelines, which may produce or enhance the spatial variability in permeability. Even if topographic stresses do not cause new fractures, they could activate and cause displacement on old fractures, making the rocks easier to erode and increasing the permeability, and potentially driving a positive feedback that enhances the growth of valley relief. Calculated stress fields are consistent with field observations, which show a rapid decline in fracture abundance with increasing depth below the valley floor, and predict a more gradual trend beneath ridgetops, leading to a more consistent (and lower) hydraulic conductivity with depth on the ridgetops when compared to the valley, where values are higher but more variable with depth. Hydraulic

  15. Effects of larval crowding on development time, survival and weight at metamorphosis in Aedes aegypti (Diptera: Culicidae Efectos del hacinamiento larval en el tiempo de desarrollo, la supervivencia y el peso en la metamorfosis de Aedes aegypti (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Arnaldo Maciá

    2009-06-01

    Full Text Available The effects of larval crowding on survival, weight at metamorphosis and development time were assessed in the dengue mosquito, Aedes aegypti L., under a controlled environment. Larval cohorts were bred at 7 different densities (4, 8, 16, 32, 64, 128 and 256 larvae / 175 ml pot, while keeping constant water volume and food amount and quality, under controlled temperature and photoperiod. Natural detritus, mainly leaves, obtained from containers naturally colonized by A. aegypti, were used as a source of nutrients for larvae. Development time, mortality, mass at metamorphosis, and total biomass were recorded for each density. Development time ranged from 4 to 23 days in males, and from 5 to 24 in females, whereby larvae took longer to develop at 64 (females and 128 (males larvae per recipient. At high densities there was a male-biased sex proportion. At densities equal to or higher than 0.4 larvae/ml (0.32 larvae/cm² there was an increase of mortality. An inverse relationship between larval density and pupal weight was detected. Biomass per individual reached asymptotic values of about 1 mg/individual at a density of 128 individuals/pot (0.64 larvae/cm². This experiment shows that this southern strain of A. aegypti is sensitive to crowding in small containers.Los efectos del hacinamiento larval sobre el tiempo de desarrollo, la supervivencia y el peso en la metamorfosis fueron estudiados en el mosquito del dengue, Aedes aegypti L., en el laboratorio. Se criaron cohortes de larvas en 7 densidades (4, 8, 16, 32, 64, 128 y 256 larvas/ recipiente de 175 ml mientras se mantuvo constante el volumen de agua y la calidad y cantidad de alimento, bajo fotoperíodo y temperatura controlados. Se usaron detritos naturales, principalmente hojas, obtenidos de contenedores colonizados naturalmente por A. aegypti como fuente de nutrientes para las larvas. En cada densidad se registraron el tiempo de desarrollo, la mortalidad, el peso en la metamorfosis y la

  16. Tingkat Kerentanan Aedes aegypti (Linn. terhadap Malation di Provinsi Sumatera Selatan

    Directory of Open Access Journals (Sweden)

    Lasbudi P. Ambarita

    2015-07-01

    Full Text Available AbstractDengue vector control program in Indonesia and also South Sumatera Province has been using malathion quite long enough. The extensive use of chemical in dengue vector control can lead to development of resistance. This study aims to determine the susceptibility of Aedes aegypti against malathion in 11 district of South Sumatera Province. Larva or pupae were collected with entomology survey kit and colonized until first generation (F1 that were used for bioassay. This test was conducted according to WHO adult susceptibility bioassay procedure.Twenty five blood-fed mosquitoes were exposed to insecticide impregnated paper in each of 4 WHO test kits and 1 control tube. Aedes aegypti from all study sites were still susceptible to operational dose of malathion (5%after 1 hour exposure. The estimated resistance ratio (ERR of knockdown time (KT to operasional dose of malathion is about 1,02 – 1,27 for KT50 and 0,96 – 1,24 for KT95. The susceptibility test of adult mosquitoes to diagnostic dose (0,8% of malathion showed a variety of susceptibility after 24 hours. Strain of 7 districts showed resistance, 3 districts toleran and 1 district still susceptible. The detection of resistance can actually help public health personnel to formulate appropriate steps in encountering the reduction in effectiveness of vector control efforts.Keywords : Aedes aegypti, Malathion, Susceptibility, South SumateraAbstrakProgram pengendalian vektor DBD di Indonesia termasuk di Provinsi Sumatera Selatan telah cukup lama menggunakan malation dengan konsentrasi 5%. Penggunaan satu jenis insektisida kimiawi secara ekstensif dapat memicu perkembangan resistensi. Penelitian ini bertujuan untuk menentukan status kerentanan Aedes aegypti terhadap malation dari 11 kabupaten/kota di Provinsi Sumatera Selatan. Larva atau pupa dikumpulkan menggunakan alat survei entomologi dan selanjutnyadipelihara hingga mendapatkan generasi pertama (F1 yang akan digunakan pada uji

  17. Uncovering the Repertoire of Endogenous Flaviviral Elements in Aedes Mosquito Genomes.

    Science.gov (United States)

    Suzuki, Yasutsugu; Frangeul, Lionel; Dickson, Laura B; Blanc, Hervé; Verdier, Yann; Vinh, Joelle; Lambrechts, Louis; Saleh, Maria-Carla

    2017-08-01

    Endogenous viral elements derived from nonretroviral RNA viruses have been described in various animal genomes. Whether they have a biological function, such as host immune protection against related viruses, is a field of intense study. Here, we investigated the repertoire of endogenous flaviviral elements (EFVEs) in Aedes mosquitoes, the vectors of arboviruses such as dengue and chikungunya viruses. Previous studies identified three EFVEs from Aedes albopictus cell lines and one from Aedes aegypti cell lines. However, an in-depth characterization of EFVEs in wild-type mosquito populations and individual mosquitoes in vivo has not been performed. We detected the full-length DNA sequence of the previously described EFVEs and their respective transcripts in several A. albopictus and A. aegypti populations from geographically distinct areas. However, EFVE-derived proteins were not detected by mass spectrometry. Using deep sequencing, we detected the production of PIWI-interacting RNA-like small RNAs, in an antisense orientation, targeting the EFVEs and their flanking regions in vivo The EFVEs were integrated in repetitive regions of the mosquito genomes, and their flanking sequences varied among mosquito populations. We bioinformatically predicted several new EFVEs from a Vietnamese A. albopictus population and observed variation in the occurrence of those elements among mosquitoes. Phylogenetic analysis of an A. aegypti EFVE suggested that it integrated prior to the global expansion of the species and subsequently diverged among and within populations. The findings of this study together reveal the substantial structural and nucleotide diversity of flaviviral integrations in Aedes genomes. Unraveling this diversity will help to elucidate the potential biological function of these EFVEs. IMPORTANCE Endogenous viral elements (EVEs) are whole or partial viral sequences integrated in host genomes. Interestingly, some EVEs have important functions for host fitness and

  18. Un juego como estrategia educativa para el control de Aedes aegypti en escolares venezolanos

    Directory of Open Access Journals (Sweden)

    Edison Vivas

    2003-12-01

    Full Text Available OBJETIVO: Evaluar el juego conocido por Jugando en salud: dengue, como medio de enseñanza-aprendizaje que favorece la incorporación de los escolares de la segunda etapa de educación básica a las actividades de control del mosquito Aedes aegypti y de prevención del dengue en el municipio de Girardot, Estado Aragua, Venezuela. MÉTODOS: Se efectuó un estudio descriptivo cuasiexperimental basado en una investigación de campo realizada en 9 escuelas seleccionadas por muestreo aleatorio sistemático entre las 29 escuelas estatales del municipio de Girardot, Estado Aragua, Venezuela, que tenían tres o más salones de sexto grado de primaria. En cada escuela se constituyeron tres salones de trabajo: en el grupo de salones no. 1 (210 estudiantes se practicó el juego tres veces por semana durante 60 días y al maestro se le entregó un material teórico sobre el dengue; en el grupo de salones no. 2 (196 estudiantes se utilizó solo el material teórico que se le entregó al maestro, y en el grupo no. 3 o grupo de control (215 estudiantes se siguió la programación habitual establecida por el Ministerio de Educación. A los escolares participantes se les realizó una evaluación antes y después de aplicar el programa propuesto, con un cuestionario que fue evaluado mediante una escala de Likert. Se calcularon las medidas de concentración y dispersión de los datos obtenidos durante las evaluaciones. Se empleó el análisis de la varianza de clasificación simple para comparar los promedios de los datos obtenidos en los distintos salones y detectar diferencias significativas entre los grupos en cuanto a conocimientos y destrezas antes y después de aplicar el programa propuesto. Se utilizó la prueba de Scheffé para determinar diferencias dentro de los grupos. RESULTADOS: En la investigación participaron 621 escolares de 8 a 16 años de edad (media: 11,8 años. De los 210 estudiantes que participaron en el juego, 41% lo valoraron ''de alta

  19. Characterization of Tolypocladium cylindrosporum (Hypocreales: Ophiocordycipitaceae) and Its Impact Against Aedes aegypti and Aedes albopictus Eggs at Low Temperature.

    Science.gov (United States)

    Flor-Weiler, Lina B; Rooney, Alejandro P; Behle, Robert W; Muturi, Ephantus J

    2017-09-01

    We examined the growth characteristics of Tolypocladium cylindrosporum IBT 41712 and its potential to infect eggs of Aedes aegypti and Ae. albopictus at a low temperature (15°C). When grown on Sabouraud dextrose agar supplemented with yeast extract, the IBT 41712 formed white colonies turning to a slightly darker, off-white color when mature. The mycelia bore swollen conidiophores producing smooth-walled, oblong to cylindrical conidia with varying sizes, ranging from 1.5 to 3.5 μm long. To determine the optimum temperature for the fungus, we cultured the fungus at eight temperatures (4°C, 12°C, 15°C, 21°C, 28°C, 33°C, 37°C, and 40°C) and measured the diametric growth. The optimum temperature for growth was 28°C since it had the highest diametric growth rate (2.1 ± 0.05 mm/day) and the fastest sporulation period (within 8-10 days of incubation). There was no fungal growth at the 3 highest temperatures (33°C, 37°C, and 40°C) but plates incubated at 33°C, when shifted to optimal temperature (28°C), showed visible growth indicating that following incubation at 33°C, the fungus remained viable. The IBT 41712 successfully infected mosquito eggs at 15°C. Fungal treatment induced egg hatch on moist seed-germination paper and this effect was more pronounced in Ae. aegypti compared to Ae. albopictus. When treated eggs were immersed in dH 2 O 21 days posttreatment, larval hatch of both Ae. aegypti (control = 91%, 1 × 10 7 conidia/ml, fungal treatment = 0%) and Ae. albopictus (control = 85%, fungal treatment = 28%) was significantly lower in fungal treatment compared to the controls. The ability of the strain to grow in a wide temperature range, and effectively infect mosquito eggs and induce egg hatch at a low temperature warrants further investigation for its potential as a mosquito control agent targeting eggs that overwinter or undergo long diapause.

  20. Informe preliminar sobre paludrina

    Directory of Open Access Journals (Sweden)

    Aquiles Peñuela Rozo

    1948-05-01

    Full Text Available En el año de 1945, Curd Davey y Rose eligieron la pirimidina como substancia de experimentación debido a que esta substancia se encuentra en las nucleo-proteinas y por su presencia en algunas sulfadrogas especial mente la sulfadiazina, de conocidas propiedades anti-palúdicas. Ensayaron la pirimidina y derivados en infestaciones por plasmodium gallinaceum en gallinas; y comprobaron que estas substancias tenían realmente acción antipalúdica. Pero al trasladar sus experiencias al hombre, encontraron que la pirimidina y sus derivados eran demasiado tóxicos. Pensando, entonces, en la similitud química del grupo de la pirimida y de la guanidina, incluyeron este último grupo en los compuestos para ensayar, descubriendo que la inclusión de la guanidina aumentaba grandemente la acción de las substancias en experiencia. Se obtuvo de esta manera un producto denominado en el numero 4-888, o paludrina, cuya fórmula o nombre químico es N. P. clorofenil N5 isopropildiguanidina, droga que se encontró muy activa contra las formas sanguíneas del Plasmodium Gallinaceum y que también se hallo activa contra las formas exoeritrocíticas del parásito. Esta capacidad de obrar sobre la fase exoeritrocítica es prácticamente exclusiva de esta substancia. Hasta hace poco tiempo esta fase exoeritrocítica, había sido demostrada solamente en trabajos de experimentación en las  aves; pero en la actualidad ha quedado también demostrada en la enfermedad humana, por experiencias del Filipino Nolasco and África, quien logro demostrar el plasmodium Vivax en el tejido retículo endotelial del pulmón de enfermos de paludismo, aunque ya Brug, en el año de 1940 había hecho comunicaciones en el mismo sentido. Se comprende la grande importancia de la paludrina, en el tratamiento del paludismo; si se considera que las recidivas de la enfermedad son debidas a la existencia de esta fase xeoeritrocítica.

  1. George William Hill, the Great but Unknown 19th Century Celestial Mechanician

    Science.gov (United States)

    Corbin, Brenda G.

    2012-01-01

    George William Hill (1838-1914) has long been considered one of the most famous and talented celestial mechanicians of the past century and a half. However, many people have never heard of him and his work. Simon Newcomb said he "will easily rank as the greatest master of mathematical astronomy during the last quarter of the nineteenth century.” After receiving a B.A. at Rutgers in 1859, Hill began work in 1861 at the office of the American Ephemeris and Nautical Almanac in Cambridge, MA. He moved to Washington with the group in 1882 which then became part of the U. S. Naval Observatory. Newcomb, beginning his work on planetary motion, assigned the theory of Jupiter and Saturn to him, calling it about the most difficult topic. Hill's work was published by the USNO in 1890 as A New Theory of Jupiter and Saturn. From 1898 to 1901, Hill lectured on the subject of celestial mechanics at Columbia University in a position created just for him. After 1892 and until his death, he lived at the family homestead in West Nyack, NY. He never married, was something of a recluse, and spent most of his time with his books and research. Hill was an amateur botanist and enjoyed exploring on long walks in the countryside. Many honors and awards came to him during his lifetime, both from the U.S. and abroad, including serving as president of the American Mathematical Society. All of Hill's mathematical and astronomical research was incorporated in The Collected Mathematical Works of George William Hill. This work, containing a preface in French by Poincare, was published in 4 large volumes by the Carnegie Institution of Washington in 1905.

  2. Spatial and Temporal Variation in Aedes aegypti and Aedes albopictus (Diptera: Culicidae) Numbers in the Yogyakarta Area of Java, Indonesia, With Implications for Wolbachia Releases.

    Science.gov (United States)

    Tantowijoyo, W; Arguni, E; Johnson, P; Budiwati, N; Nurhayati, P I; Fitriana, I; Wardana, S; Ardiansyah, H; Turley, A P; Ryan, P; O'Neill, S L; Hoffmann, A A

    2016-01-01

    of mosquito vector populations, particularly through Wolbachia endosymbionts. The success of these strategies depends on understanding the dynamics of vector populations. In preparation for Wolbachia releases around Yogyakarta, we have studied Aedes populations in five hamlets. Adult monitoring with BioGent- Sentinel (BG-S) traps indicated that hamlet populations had different dynamics across the year; while there was an increase in Aedes aegypti (L.) and Aedes albopictus (Skuse) numbers in the wet season, species abundance remained relatively stable in some hamlets but changed markedly (>2 fold) in others. Local rainfall a month prior to monitoring partly predicted numbers of Ae. aegypti but not Ae. albopictus. Site differences in population size indicated by BG-S traps were also evident in ovitrap data. Egg or larval collections with ovitraps repeated at the same location suggested spatial autocorrelation (<250 m) in the areas of the hamlets where Ae. aegypti numbers were high. Overall, there was a weak negative association (r<0.43) between Ae. aegypti and Ae. albopictus numbers in ovitraps when averaged across collections. Ae. albopictus numbers in ovitraps and BG-S traps were positively correlated with vegetation around areas where traps were placed, while Ae. aegypti were negatively correlated with this feature. These data inform intervention strategies by defining periods when mosquito densities are high, highlighting the importance of local site characteristics on populations, and suggesting relatively weak interactions between Ae. aegypti and Ae. albopictus. They also indicate local areas within hamlets where consistently high mosquito densities may influence Wolbachia invasions and other interventions.

  3. Identification of entomopathogenic nematodes and symbiotic bacteria from Nam Nao National Park in Thailand and larvicidal activity of symbiotic bacteria against Aedes aegypti and Aedes albopictus.

    Science.gov (United States)

    Yooyangket, Temsiri; Muangpat, Paramaporn; Polseela, Raxsina; Tandhavanant, Sarunporn; Thanwisai, Aunchalee; Vitta, Apichat

    2018-01-01

    Entomopathogenic nematodes (EPNs) that are symbiotically associated with Xenorhabdus and Photorhabdus bacteria can kill target insects via direct infection and toxin action. There are limited reports identifying such organisms in the National Park of Thailand. Therefore, the objectives of this study were to identify EPNs and symbiotic bacteria from Nam Nao National Park, Phetchabun Province, Thailand and to evaluate the larvicidal activity of bacteria against Aedes aegypti and Ae. albopictus. A total of 12 EPN isolates belonging to Steinernema and Heterorhabditis were obtained form 940 soil samples between February 2014 and July 2016. EPNs were molecularly identified as S. websteri (10 isolates) and H. baujardi (2 isolates). Symbiotic bacteria were isolated from EPNs and molecularly identified as P. luminescens subsp. akhurstii (13 isolates), X. stockiae (11 isolates), X. vietnamensis (2 isolates) and X. japonica (1 isolate). For the bioassay, bacterial suspensions were evaluated for toxicity against third to early fourth instar larvae of Aedes spp. The larvae of both Aedes species were orally susceptible to symbiotic bacteria. The highest larval mortality of Ae. aegypti was 99% after exposure to X. stockiae (bNN112.3_TH) at 96 h, and the highest mortality of Ae. albopictus was 98% after exposure to P. luminescens subsp. akhurstii (bNN121.4_TH) at 96 h. In contrast to the control groups (Escherichia coli and distilled water), the mortality rate of both mosquito larvae ranged between 0 and 7% at 72 h. Here, we report the first observation of X. vietnamensis in Thailand. Additionally, we report the first observation of P. luminescens subsp. akhurstii associated with H. baujardi in Thailand. X. stockiae has potential to be a biocontrol agent for mosquitoes. This investigation provides a survey of the basic diversity of EPNs and symbiotic bacteria in the National Park of Thailand, and it is a bacterial resource for further studies of bioactive compounds.

  4. Produtividade de criadouro de Aedes albopictus em ambiente urbano Productivity of container-breeding Aedes albopictus in an urban environment

    Directory of Open Access Journals (Sweden)

    Oswaldo Paulo Forattini

    1997-12-01

    Full Text Available INTRODUÇÃO: O encontro de Aedes albopictus na cidade de Cananéia, região Sudeste do Estado de São Paulo, Brasil, ensejou a ocasião de realizar observações que visassem avaliar a produtividade de criadouro grande e permanente. Como objetivo, após selecionar o habitat a ser estudado, tentou-se avaliar-lhe a contribuição para a densidade local do mosquito. MATERIAL E MÉTODO: Em área predeterminada procedeu-se a levantamento de criadouros potenciais. Constatada a presença da espécie, foi selecionado um dos recipientes que preenchia os requisitos desejados. O acompanhamento foi feito de maneira ininterrupta, no período de novembro de 1996 a maio de 1997. As observações obdeceram a ritmo quinzenal retirando, cada vez, amostra da água correspondente a 0,14, ou seja, um sétimo do volume total de 70 litros. Procurou-se coletar, identificar e numerar, por sexo, as pupas existentes. Concomitantemente, procedeu-se à captura de formas adultas. Foi utilizada a isca humana das 15:00 às 18:00h, instalada a cerca de 6 metros do mencionado criadouro. Finda essa coleta, foi feita aspiração com 30 min. de duração em locais de abrigo representados pela abundante vegetação circunjacente. RESULTADOS: Nas coletas de formas imaturas do criadouro, o Ae. albopictus compareceu com 44,9%. Ao longo de 15 amostras regularmente realizadas obteve-se a média de 31,13 pupas pertencentes a essa espécie. O índice de emergência(E foi de 2,1. A multiplicação desse valor por sete forneceu a média diária de 14,7 fêmeas. Nas coletas de adultos desse sexo, a média de Williams para a isca humana foi de 30,7, enquanto a densidade média horária da aspiração dos locais de abrigo foi de 9,2. O cálculo do acúmulo diário concluiu pela presença de 22,8 fêmeas, por dia, capazes de freqüentar a isca humana, nessa situação e condições. DISCUSSÃO: A contagem de pupas possibilitou estimar a produtividade de criadouro de Ae. albopictus, tipo grande

  5. Hill functions for stochastic gene regulatory networks from master equations with split nodes and time-scale separation

    Science.gov (United States)

    Lipan, Ovidiu; Ferwerda, Cameron

    2018-02-01

    The deterministic Hill function depends only on the average values of molecule numbers. To account for the fluctuations in the molecule numbers, the argument of the Hill function needs to contain the means, the standard deviations, and the correlations. Here we present a method that allows for stochastic Hill functions to be constructed from the dynamical evolution of stochastic biocircuits with specific topologies. These stochastic Hill functions are presented in a closed analytical form so that they can be easily incorporated in models for large genetic regulatory networks. Using a repressive biocircuit as an example, we show by Monte Carlo simulations that the traditional deterministic Hill function inaccurately predicts time of repression by an order of two magnitudes. However, the stochastic Hill function was able to capture the fluctuations and thus accurately predicted the time of repression.

  6. Repellent activity of herbal essential oils against Aedes aegypti (Linn. and Culex quinquefasciatus (Say.

    Directory of Open Access Journals (Sweden)

    Duangkamon Sritabutra

    2013-08-01

    Full Text Available Objective: To determine the mosquito repellent activity of herbal essential oils against female Aedes aegypti and Culex quinquefasciatus. Methods: On a volunteer’s forearm, 0.1 mL of each essential oil was applied to 3 cm伊10 cm of exposed skin. The protection time was recorded for 3 min after every 30 min. Results: Essential oil from clove oil in olive oil and in coconut oil gave the longest lasting period of 76.50 min and 96.00 min respectively against Aedes aegypti. The citronella grass oil in olive oil, citronella grass oil in coconut oil and lemongrass oil in coconut oil exhibited protection against Culex quinquefasciatus at 165.00, 105.00, and 112.50 min respectively. Conclusions: The results clearly indicated that clove, citronella and lemongrass oil were the most promising for repellency against mosquito species. These oils could be used to develop a new formulation to control mosquitoes.

  7. Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.

    Directory of Open Access Journals (Sweden)

    Roya Elaine Haghighat-Khah

    Full Text Available Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests.

  8. Indeks Pertumbuhan Larva Aedes aegypti L. Yang Terdedah Dalam Ekstrak Air Kulit Jengkol (Pithecellobium lobatum

    Directory of Open Access Journals (Sweden)

    Firda Yanuar Pradani

    2013-02-01

    Full Text Available Abstracts. The control of dengue fever (DBD vector generally use the synthetic insecti­cides, however it's utilization had negative effect to the environment. Jengkol (Pithecellobium lobaturn was one of the plants which could be used as larvacide alternative because it had phenolate acid, alkaloid, terpenoid and saponin in its rind. This research was conducted to know the growth index of Aedes aegypti larvae which was soaked in the extract ofP. lobaturn rind. This experiment used P. lobaturn rind ekstract at concentration 36%, 18%, 9% and 0% . The data were analyzed by using Zhang et.al methode (1993. The P. lobaturn rind extract were toxic to larvae especially in first and second instar at concentration 17,94% respectively. Relativelly growth index range from 0,817-1, it is mean that some larvae were stuned, and some were growth into the next phase.Key words: Aedes aegypti, Jengkol, LCso, growth index.

  9. Oral susceptibility of Aedes aegypti (Diptera: Culicidae) from Senegal for dengue serotypes 1 and 3 viruses.

    Science.gov (United States)

    Gaye, Alioune; Faye, Oumar; Diagne, Cheikh T; Faye, Ousmane; Diallo, Diawo; Weaver, Scott C; Sall, Amadou A; Diallo, Mawlouth

    2014-11-01

    To investigate the potential for domestic and wild populations of Aedes aegypti from Dakar and Kedougou to develop a disseminated infection after exposure to DENV-3 and DENV-1. We have exposed sylvatic and urban population of Ae. aegypti from Senegal to bloomeals containing dengue serotype 1 and 3. At different incubation period, individual mosquito legs/wings and bodies were tested for virus presence using real time RT-PCR to estimate the infection and dissemination rates. The data indicated low susceptibility to DENV-3 (infection: 2.4-15.2%, and dissemination rates: 0-8.3%) and higher susceptibility to DENV-1 (infection and dissemination rates up to 50%). Aedes aegypti from Senegal seem able to develop a disseminated infection of DENV-1 and DENV-3. Further studies are needed to test their ability to transmit the two serotypes. © 2014 John Wiley & Sons Ltd.

  10. Wolbachia Blocks Currently Circulating Zika Virus Isolates in Brazilian Aedes aegypti Mosquitoes.

    Science.gov (United States)

    Dutra, Heverton Leandro Carneiro; Rocha, Marcele Neves; Dias, Fernando Braga Stehling; Mansur, Simone Brutman; Caragata, Eric Pearce; Moreira, Luciano Andrade

    2016-06-08

    The recent association of Zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the Zika vector, Aedes aegypti mosquitoes. Wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. Here we report that Aedes aegypti harboring Wolbachia are highly resistant to infection with two currently circulating Zika virus isolates from the recent Brazilian epidemic. Wolbachia-harboring mosquitoes displayed lower viral prevalence and intensity and decreased disseminated infection and, critically, did not carry infectious virus in the saliva, suggesting that viral transmission was blocked. Our data indicate that the use of Wolbachia-harboring mosquitoes could represent an effective mechanism to reduce Zika virus transmission and should be included as part of Zika control strategies. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  11. Site-Specific Cassette Exchange Systems in the Aedes aegypti Mosquito and the Plutella xylostella Moth

    Science.gov (United States)

    Haghighat-Khah, Roya Elaine; Scaife, Sarah; Martins, Sara; St John, Oliver; Matzen, Kelly Jean; Morrison, Neil; Alphey, Luke

    2015-01-01

    Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE) mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests. PMID:25830287

  12. Produtividade de criadouro de Aedes albopictus em ambiente urbano

    Directory of Open Access Journals (Sweden)

    Forattini Oswaldo Paulo

    1997-01-01

    Full Text Available INTRODUÇÃO: O encontro de Aedes albopictus na cidade de Cananéia, região Sudeste do Estado de São Paulo, Brasil, ensejou a ocasião de realizar observações que visassem avaliar a produtividade de criadouro grande e permanente. Como objetivo, após selecionar o habitat a ser estudado, tentou-se avaliar-lhe a contribuição para a densidade local do mosquito. MATERIAL E MÉTODO: Em área predeterminada procedeu-se a levantamento de criadouros potenciais. Constatada a presença da espécie, foi selecionado um dos recipientes que preenchia os requisitos desejados. O acompanhamento foi feito de maneira ininterrupta, no período de novembro de 1996 a maio de 1997. As observações obdeceram a ritmo quinzenal retirando, cada vez, amostra da água correspondente a 0,14, ou seja, um sétimo do volume total de 70 litros. Procurou-se coletar, identificar e numerar, por sexo, as pupas existentes. Concomitantemente, procedeu-se à captura de formas adultas. Foi utilizada a isca humana das 15:00 às 18:00h, instalada a cerca de 6 metros do mencionado criadouro. Finda essa coleta, foi feita aspiração com 30 min. de duração em locais de abrigo representados pela abundante vegetação circunjacente. RESULTADOS: Nas coletas de formas imaturas do criadouro, o Ae. albopictus compareceu com 44,9%. Ao longo de 15 amostras regularmente realizadas obteve-se a média de 31,13 pupas pertencentes a essa espécie. O índice de emergência(E foi de 2,1. A multiplicação desse valor por sete forneceu a média diária de 14,7 fêmeas. Nas coletas de adultos desse sexo, a média de Williams para a isca humana foi de 30,7, enquanto a densidade média horária da aspiração dos locais de abrigo foi de 9,2. O cálculo do acúmulo diário concluiu pela presença de 22,8 fêmeas, por dia, capazes de freqüentar a isca humana, nessa situação e condições. DISCUSSÃO: A contagem de pupas possibilitou estimar a produtividade de criadouro de Ae. albopictus, tipo grande

  13. Produtividade de criadouro de Aedes albopictus em ambiente urbano

    Directory of Open Access Journals (Sweden)

    Oswaldo Paulo Forattini

    1997-12-01

    Full Text Available INTRODUÇÃO: O encontro de Aedes albopictus na cidade de Cananéia, região Sudeste do Estado de São Paulo, Brasil, ensejou a ocasião de realizar observações que visassem avaliar a produtividade de criadouro grande e permanente. Como objetivo, após selecionar o habitat a ser estudado, tentou-se avaliar-lhe a contribuição para a densidade local do mosquito. MATERIAL E MÉTODO: Em área predeterminada procedeu-se a levantamento de criadouros potenciais. Constatada a presença da espécie, foi selecionado um dos recipientes que preenchia os requisitos desejados. O acompanhamento foi feito de maneira ininterrupta, no período de novembro de 1996 a maio de 1997. As observações obdeceram a ritmo quinzenal retirando, cada vez, amostra da água correspondente a 0,14, ou seja, um sétimo do volume total de 70 litros. Procurou-se coletar, identificar e numerar, por sexo, as pupas existentes. Concomitantemente, procedeu-se à captura de formas adultas. Foi utilizada a isca humana das 15:00 às 18:00h, instalada a cerca de 6 metros do mencionado criadouro. Finda essa coleta, foi feita aspiração com 30 min. de duração em locais de abrigo representados pela abundante vegetação circunjacente. RESULTADOS: Nas coletas de formas imaturas do criadouro, o Ae. albopictus compareceu com 44,9%. Ao longo de 15 amostras regularmente realizadas obteve-se a média de 31,13 pupas pertencentes a essa espécie. O índice de emergência(E foi de 2,1. A multiplicação desse valor por sete forneceu a média diária de 14,7 fêmeas. Nas coletas de adultos desse sexo, a média de Williams para a isca humana foi de 30,7, enquanto a densidade média horária da aspiração dos locais de abrigo foi de 9,2. O cálculo do acúmulo diário concluiu pela presença de 22,8 fêmeas, por dia, capazes de freqüentar a isca humana, nessa situação e condições. DISCUSSÃO: A contagem de pupas possibilitou estimar a produtividade de criadouro de Ae. albopictus, tipo grande

  14. Soil Health Management under Hill Agroecosystem of North East India

    Directory of Open Access Journals (Sweden)

    R. Saha

    2012-01-01

    Full Text Available The deterioration of soil quality/health is the combined result of soil fertility, biological degradation (decline of organic matter, biomass C, decrease in activity and diversity of soil fauna, increase in erodibility, acidity, and salinity, and exposure of compact subsoil of poor physicochemical properties. Northeast India is characterized by high soil acidity/Al+3 toxicity, heavy soil, and carbon loss, severe water scarcity during most parts of year though it is known as high rainfall area. The extent of soil and nutrient transfer, causing environmental degradation in North eastern India, has been estimated to be about 601 million tones of soil, and 685.8, 99.8, 511.1, 22.6, 14.0, 57.1, and 43.0 thousand tones of N, P, K, Mn, Zn, Ca, and Mg, respectively. Excessive deforestation coupled with shifting cultivation practices have resulted in tremendous soil loss (200 t/ha/yr, poor soil physical health in this region. Studies on soil erodibility characteristics under various land use systems in Northeastern Hill (NEH Region depicted that shifting cultivation had the highest erosion ratio (12.46 and soil loss (30.2–170.2 t/ha/yr, followed by conventional agriculture system (10.42 and 5.10–68.20 t/ha/yr, resp.. The challenge before us is to maintain equilibrium between resources and their use to have a stable ecosystem. Agroforestry systems like agri-horti-silvi-pastoral system performed better over shifting cultivation in terms of improvement in soil organic carbon; SOC (44.8%, mean weight diameter; MWD (29.4%, dispersion ratio (52.9%, soil loss (99.3%, soil erosion ratio (45.9%, and in-situ soil moisture conservation (20.6% under the high rainfall, moderate to steep slopes, and shallow soil depth conditions. Multipurpose trees (MPTs also played an important role on soil rejuvenation. Michelia oblonga is reported to be a better choice as bioameliorant for these soils as continuous leaf litter and root exudates improved soil physical

  15. Landslide susceptibility estimations in the Gerecse hills (Hungary).

    Science.gov (United States)

    Gerzsenyi, Dávid; Gáspár, Albert

    2017-04-01

    Surface movement processes are constantly posing threat to property in populated and agricultural areas in the Gerecse hills (Hungary). The affected geological formations are mainly unconsolidated sediments. Pleistocene loess and alluvial terrace sediments are overwhelmingly present, but fluvio-lacustrine sediments of the latest Miocene, and consolidated Eocene and Mesozoic limestones and marls can also be found in the area. Landslides and other surface movement processes are being studied for a long time in the area, but a comprehensive GIS-based geostatistical analysis have not yet been made for the whole area. This was the reason for choosing the Gerecse as the focus area of the study. However, the base data of our study are freely accessible from online servers, so the used method can be applied to other regions in Hungary. Qualitative data was acquired from the landslide-inventory map of the Hungarian Surface Movement Survey and from the Geological Map of Hungary (1 : 100 000). Morphometric parameters derived from the SRMT-1 DEM were used as quantitative variables. Using these parameters the distribution of elevation, slope gradient, aspect and categorized geological features were computed, both for areas affected and not affected by slope movements. Then likelihood values were computed for each parameters by comparing their distribution in the two areas. With combining the likelihood values of the four parameters relative hazard values were computed for each cell. This method is known as the "empirical probability estimation" originally published by Chung (2005). The map created this way shows each cell's place in their ranking based on the relative hazard values as a percentage for the whole study area (787 km2). These values provide information about how similar is a certain area to the areas already affected by landslides based on the four predictor variables. This map can also serve as a base for more complex landslide vulnerability studies involving

  16. The Aquaporin Gene Family of the Yellow Fever Mosquito, Aedes aegypti

    OpenAIRE

    Drake, Lisa L.; Boudko, Dmitri Y.; Marinotti, Osvaldo; Carpenter, Victoria K.; Dawe, Angus L.; Hansen, Immo A.

    2010-01-01

    Background The mosquito, Aedes aegypti, is the principal vector of the Dengue and yellow fever viruses. During feeding, an adult female can take up more than its own body weight in vertebrate blood. After a blood meal females excrete large amounts of urine through their excretion system, the Malpighian tubules (MT). Diuresis starts within seconds after the mosquito starts feeding. Aquaporins (AQPs) are a family of membrane transporters that regulate the flow of water, glycerol and other small...

  17. The developmental transcriptome of the mosquito Aedes aegypti, an invasive species and major arbovirus vector.

    Science.gov (United States)

    Akbari, Omar S; Antoshechkin, Igor; Amrhein, Henry; Williams, Brian; Diloreto, Race; Sandler, Jeremy; Hay, Bruce A

    2013-09-04

    Mosquitoes are vectors of a number of important human and animal diseases. The development of novel vector control strategies requires a thorough understanding of mosquito biology. To facilitate this, we used RNA-seq to identify novel genes and provide the first high-resolution view of the transcriptome throughout development and in response to blood feeding in a mosquito vector of human disease, Aedes aegypti, the primary vector for Dengue and yellow fever. We characterized mRNA expression at 34 distinct time points throughout Aedes development, including adult somatic and germline tissues, by using polyA+ RNA-seq. We identify a total of 14,238 novel new transcribed regions corresponding to 12,597 new loci, as well as many novel transcript isoforms of previously annotated genes. Altogether these results increase the annotated fraction of the transcribed genome into long polyA+ RNAs by more than twofold. We also identified a number of patterns of shared gene expression, as well as genes and/or exons expressed sex-specifically or sex-differentially. Expression profiles of small RNAs in ovaries, early embryos, testes, and adult male and female somatic tissues also were determined, resulting in the identification of 38 new Aedes-specific miRNAs, and ~291,000 small RNA new transcribed regions, many of which are likely to be endogenous small-interfering RNAs and Piwi-interacting RNAs. Genes of potential interest for transgene-based vector control strategies also are highlighted. Our data have been incorporated into a user-friendly genome browser located at www.Aedes.caltech.edu, with relevant links to Vectorbase (www.vectorbase.org).

  18. Construction of a field trap for initiating an ovipositional response in Aedes taeniorhynchus.

    Science.gov (United States)

    Wallace, F L

    1996-09-01

    An oviposition trap was constructed for the black saltmarsh mosquito, Aedes taeniorhynchus. The trap consisted of a 50 x 60-cm piece of contaminated 100% cotton bath towel, saturated with 85% tap water, a container, and a cover of dried plant parts placed over the contaminated toweling. This combination initiated oviposition. Contamination of the toweling was due to populations of bacteria and fungi. The eggs recovered were free from soil and debris.

  19. Oral infection of Aedes aegypti with yellow fever virus: geographic variation and genetic considerations.

    Science.gov (United States)

    Tabachnick, W J; Wallis, G P; Aitken, T H; Miller, B R; Amato, G D; Lorenz, L; Powell, J R; Beaty, B J

    1985-11-01

    Twenty-eight populations representing a worldwide distribution of Aedes aegypti were tested for their ability to become orally infected with yellow fever virus (YFV). Populations had been analyzed for genetic variations at 11 isozyme loci and assigned to one of 8 genetic geographic groups of Ae. aegypti. Infection rates suggest that populations showing isozyme genetic relatedness also demonstrate similarity to oral infection rates with YFV. The findings support the hypothesis that genetic variation exists for oral susceptibility to YFV in Ae. aegypti.

  20. Family level variation in Wolbachia-mediated dengue virus blocking in Aedes aegypti

    OpenAIRE

    Terradas, Gerard; Allen, Scott L.; Chenoweth, Stephen F.; McGraw, Elizabeth A.

    2017-01-01

    Background The mosquito vector Aedes aegypti is responsible for transmitting a range of arboviruses including dengue (DENV) and Zika (ZIKV). The global reach of these viruses is increasing due to an expansion of the mosquito’s geographic range and increasing urbanization and human travel. Vector control remains the primary means for limiting these diseases. Wolbachia pipientis is an endosymbiotic bacterium of insects that has the ability to block the replication of pathogens, including flaviv...

  1. Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia.

    Science.gov (United States)

    Rohani, A; Aidil Azahary, A R; Malinda, M; Zurainee, M N; Rozilawati, H; Wan Najdah, W M A; Lee, H L

    2014-12-01

    BACKGROUND & OBJECTIVESI: Transovarial transmission of dengue virus in the Aedes vectors is now a well-documented phenomenon reported from many parts of the endemic areas in the world, which played an important role in initiating and maintaining the outbreak in human populations. This study investigated the factors affecting breeding habitats and the relationship with transovarial dengue virus in larvae of Aedes aegypti and Ae. albopictus. Larval surveillance was conducted in dengue outbreak areas in Malaysia from 2008 until 2009. Sampling was carried out based on habitat type, water condition (substrate type), canopy coverage, temperature and pH at breeding habitats. RT-PCR was performed to detect presence of transovarial dengue virus in larvae collected in the study areas. A total of 789 breeding habitats were identified during this study and the majority of these breeding sites were plastic containers (57.46%). Aedes albopictus dominated most of the water condition surveyed, while Ae. aegypti indicated preference toward habitats with clear water. Aedes aegypti was selective in selecting ovipositional sites compared to Ae. albopictus where shaded areas were shown to be the most preferred. From a total of 363 mosquito larvae pools, 23 (6.3%) pools were positive for dengue virus where 18 of them were from Ae. albopictus and five were from Ae. aegypti mosquito larvae pools. This study indicated the presence of transovarial transmission of dengue virus in immature Ae. aegypti and Ae. albopictus in the field. This study also showed that combination of water conditions, canopy coverage, temperature and pH of breeding habitats were the factors affecting the larval population. The study suggested that larval survey programme could serve as a tool not only to monitor the local dengue vector distribution but also to provide objective information for taking appropriate action by the community against dengue vectors.

  2. Notes on the blood-feeding behavior of Aedes albopictus (Diptera : Culicidae) in Cameroon

    OpenAIRE

    Kamgang, Basile; Nchoutpouen, Elysée; Simard, Frédéric; Paupy, Christophe

    2012-01-01

    Abstract Background The invasive mosquito Aedes albopictus is often considered a poor vector of human pathogens, owing to its catholic feeding behavior. However, it was recently incriminated as a major vector in several Chikungunya epidemics, outside of its native range. Here we assessed two key elements of feeding behavior by Ae. albopictus females in Yaoundé, Cameroon, Central Africa. Host preference was explored and the human-biting activity of females was monitored over 24 h to determine ...

  3. miRNA genes of an invasive vector mosquito, Aedes albopictus.

    Directory of Open Access Journals (Sweden)

    Jinbao Gu

    Full Text Available Aedes albopictus, a vector of Dengue and Chikungunya viruses, is a robust invasive species in both tropical and temperate environments. MicroRNAs (miRNAs regulate gene expression and biological processes including embryonic development, innate immunity and infection. While a number of miRNAs have been discovered in some mosquitoes, no comprehensive effort has been made to characterize them from different developmental stages from a single species. Systematic analysis of miRNAs in Ae. albopictus will improve our understanding of its basic biology and inform novel strategies to prevent virus transmission. Between 10-14 million Illumina sequencing reads per sample were obtained from embryos, larvae, pupae, adult males, sugar-fed and blood-fed adult females. A total of 119 miRNA genes represented by 215 miRNA or miRNA star (miRNA* sequences were identified, 15 of which are novel. Eleven, two, and two of the newly-discovered miRNA genes appear specific to Aedes, Culicinae, and Culicidae, respectively. A number of miRNAs accumulate predominantly in one or two developmental stages and the large number that showed differences in abundance following a blood meal likely are important in blood-induced mosquito biology. Gene Ontology (GO analysis of the targets of all Ae. albopictus miRNAs provides a useful starting point for the study of their functions in mosquitoes. This study is the first systematic analysis of miRNAs based on deep-sequencing of small RNA samples of all developmental stages of a mosquito species. A number of miRNAs are related to specific physiological states, most notably, pre- and post-blood feeding. The distribution of lineage-specific miRNAs is consistent with mosquito phylogeny and the presence of a number of Aedes-specific miRNAs likely reflects the divergence between the Aedes and Culex genera.

  4. New insights into HCV replication in original cells from Aedes mosquitoes.

    Science.gov (United States)

    Fallecker, Catherine; Caporossi, Alban; Rechoum, Yassine; Garzoni, Frederic; Larrat, Sylvie; François, Olivier; Fender, Pascal; Morand, Patrice; Berger, Imre; Petit, Marie-Anne; Drouet, Emmanuel

    2017-08-22

    The existing literature about HCV association with, and replication in mosquitoes is extremely poor. To fill this gap, we performed cellular investigations aimed at exploring (i) the capacity of HCV E1E2 glycoproteins to bind on Aedes mosquito cells and (ii) the ability of HCV serum particles (HCVsp) to replicate in these cell lines. First, we used purified E1E2 expressing baculovirus-derived HCV pseudo particles (bacHCVpp) so we could investigate their association with mosquito cell lines from Aedes aegypti (Aag-2) and Aedes albopictus (C6/36). We initiated a series of infections of both mosquito cells (Ae aegypti and Ae albopictus) with the HCVsp (Lat strain - genotype 3) and we observed the evolution dynamics of viral populations within cells over the course of infection via next-generation sequencing (NGS) experiments. Our binding assays revealed bacHCVpp an association with the mosquito cells, at comparable levels obtained with human hepatocytes (HepaRG cells) used as a control. In our infection experiments, the HCV RNA (+) were detectable by RT-PCR in the cells between 21 and 28 days post-infection (p.i.). In human hepatocytes HepaRG and Ae aegypti insect cells, NGS experiments revealed an increase of global viral diversity with a selection for a quasi-species, suggesting a structuration of the population with elimination of deleterious mutations. The evolutionary pattern in Ae albopictus insect cells is different (stability of viral diversity and polymorphism). These results demonstrate for the first time that natural HCV could really replicate within Aedes mosquitoes, a discovery which may have major consequences for public health as well as in vaccine development.

  5. Rotenoids from Tephrosia toxicaria with larvicidal activity against Aedes aegypti, the main vector of dengue fever

    International Nuclear Information System (INIS)

    Vasconcelos, Jackson Nunes e; Santiago, Gilvandete Maria Pinheiro; Lima, Jefferson Queiroz; Arriaga, Angela Martha Campos

    2012-01-01

    In the search for new larvicides from plants, we have investigated the potential activity of the rotenoids deguelin (1), 12a-hydroxy-a-toxicarol (2) and tephrosin (3), isolated from the bioactive ethanol extract of roots of Tephrosia toxicaria Pers., against Aedes aegypti, the main vector of dengue. The absolute configuration of these compounds was determined by circular dichroism (CD) spectra. The LC50 values of the compounds evaluated justify the potential of T. toxicaria as a new natural larvicide. (author)

  6. Effect of an intervention in storm drains to prevent Aedes aegypti reproduction in Salvador, Brazil.

    Science.gov (United States)

    Souza, Raquel Lima; Mugabe, Vánio André; Paploski, Igor Adolfo Dexheimer; Rodrigues, Moreno S; Moreira, Patrícia Sousa Dos Santos; Nascimento, Leile Camila Jacob; Roundy, Christopher Michael; Weaver, Scott C; Reis, Mitermayer Galvão; Kitron, Uriel; Ribeiro, Guilherme Sousa

    2017-07-11

    Aedes aegypti, the principal vector for dengue, chikungunya and Zika viruses, is a synanthropic species that uses stagnant water to complete its reproductive cycle. In urban settings, rainfall water draining structures, such as storm drains, may retain water and serve as a larval development site for Aedes spp. reproduction. Herein, we describe the effect of a community-based intervention on preventing standing water accumulation in storm drains and their consequent infestation by adult and immature Ae. aegypti and other mosquitoes. Between April and May of 2016, local residents association of Salvador, Brazil, after being informed of water accumulation and Ae. aegypti infestation in the storm drains in their area, performed an intervention on 52 storm drains. The intervention consisted of placing concrete at the bottom of the storm drains to elevate their base to the level of the outflow tube, avoiding water accumulation, and placement of a metal mesh covering the outflow tube to avoid its clogging with debris. To determine the impact of the intervention, we compared the frequency at which the 52 storm drains contained water, as well as adult and immature mosquitoes using data from two surveys performed before and two surveys performed after the intervention. During the pre-intervention period, water accumulated in 48 (92.3%) of the storm drains, and immature Ae. aegypti were found in 11 (21.2%) and adults in 10 (19.2%). After the intervention, water accumulated in 5 (9.6%) of the storm drains (P Aedes mosquitoes (mainly Culex spp.) in the storm drains also decreased after the intervention. This study exemplifies how a simple intervention targeting storm drains can result in a major reduction of water retention, and, consequently, impact Ae. aegypti larval populations. Larger and multi-center evaluations are needed to confirm the potential of citywide structural modifications of storm drains to reduce Aedes spp. infestation level.

  7. Experimental studies of susceptibility of Italian Aedes albopictus to Zika virus.

    Science.gov (United States)

    Di Luca, Marco; Severini, Francesco; Toma, Luciano; Boccolini, Daniela; Romi, Roberto; Remoli, Maria Elena; Sabbatucci, Michela; Rizzo, Caterina; Venturi, Giulietta; Rezza, Giovanni; Fortuna, Claudia

    2016-05-05

    We report a study on vector competence of an Italian population of Aedes albopictus for Zika virus (ZIKV). Ae. albopictus was susceptible to ZIKV infection (infection rate: 10%), and the virus could disseminate and was secreted in the mosquito's saliva (dissemination rate: 29%; transmission rate: 29%) after an extrinsic incubation period of 11 days. The observed vector competence was lower than that of an Ae. aegypti colony tested in parallel.

  8. Assessing the potential risk of Zika virus epidemics in temperate areas with established Aedes albopictus populations.

    Science.gov (United States)

    Guzzetta, Giorgio; Poletti, Piero; Montarsi, Fabrizio; Baldacchino, Frederic; Capelli, Gioia; Rizzoli, Annapaola; Rosà, Roberto; Merler, Stefano

    2016-04-14

    Based on 2015 abundance of Aedes albopictus in nine northern Italian municipalities with temperate continental/oceanic climate, we estimated the basic reproductive number R0 for Zika virus (ZIKV) to be systematically below the epidemic threshold in most scenarios. Results were sensitive to the value of the probability of mosquito infection after biting a viraemic host. Therefore, further studies are required to improve models and predictions, namely evaluating vector competence and potential non-vector transmissions.

  9. Evaluation of Aedes Aegypti Presence and Abundance in Septic Tanks and Their Impacts on Dengue Transmission

    Science.gov (United States)

    2009-03-10

    aforementioned applications, GIS has been used for cost analysis decisions in vector control planning . GIS software was used to remotely identify... plan for dengue outbreaks, a contingency plan to hospitalize large numbers of dengue hemorrhagic patients if necessary, education of the medical...de nicho en tres culicidae urbanos (Culex fatigans Weid, C. coringer Theo, y Aedes aegypti L.) en el cemeterio de Caracas. Acta Cientifica

  10. How does the dengue vector mosquito Aedes albopictus respond to global warming?

    OpenAIRE

    Jia, Pengfei; Chen, Xiang; Chen, Jin; Lu, Liang; Liu, Qiyong; Tan, Xiaoyue

    2017-01-01

    Background Global warming has a marked influence on the life cycle of epidemic vectors as well as their interactions with human beings. The Aedes albopictus mosquito as the vector of dengue fever surged exponentially in the last decade, raising ecological and epistemological concerns of how climate change altered its growth rate and population dynamics. As the global warming pattern is considerably uneven across four seasons, with a confirmed stronger effect in winter, an emerging need arises...

  11. Synergistic Efficacy of Aedes aegypti Antimicrobial Peptide Cecropin A2 and Tetracycline against Pseudomonas aeruginosa

    OpenAIRE

    Zheng, Zhaojun; Tharmalingam, Nagendran; Liu, Qingzhong; Jayamani, Elamparithi; Kim, Wooseong; Fuchs, Beth Burgwyn; Zhang, Rijun; Vilcinskas, Andreas; Mylonakis, Eleftherios

    2017-01-01

    The increasing prevalence of antibiotic resistance has created an urgent need for alternative drugs with new mechanisms of action. Antimicrobial peptides (AMPs) are promising candidates that could address the spread of multidrug-resistant bacteria, either alone or in combination with conventional antibiotics. We studied the antimicrobial efficacy and bactericidal mechanism of cecropin A2, a 36-residue α-helical cationic peptide derived from Aedes aegypti cecropin A, focusing on the common pat...

  12. Contact Irritant Responses of Aedes aegypti Using Sublethal Concentration and Focal Application of Pyrethroid Chemicals

    OpenAIRE

    Manda, Hortance; Shah, Pankhil; Polsomboon, Suppaluck; Chareonviriyaphap, Theeraphap; Castro-Llanos, Fanny; Morrison, Amy; Burrus, Roxanne G.; Grieco, John P.; Achee, Nicole L.

    2013-01-01

    BACKGROUND: Previous studies have demonstrated contact irritant and spatial repellent behaviors in Aedes aegypti following exposure to sublethal concentrations of chemicals. These sublethal actions are currently being evaluated in the development of a push-pull strategy for Ae. aegypti control. This study reports on mosquito escape responses after exposure to candidate chemicals for a contact irritant focused push-pull strategy using varying concentrations and focal application. METHODS: Cont...

  13. Determinants of Heterogeneous Blood Feeding Patterns by Aedes aegypti in Iquitos, Peru

    OpenAIRE

    Liebman, Kelly A.; Stoddard, Steven T.; Reiner, Robert C.; Perkins, T. Alex; Astete, Helvio; Sihuincha, Moises; Halsey, Eric S.; Kochel, Tadeusz J.; Morrison, Amy C.; Scott, Thomas W.

    2014-01-01

    Background Heterogeneous mosquito biting results in different individuals in a population receiving an uneven number of bites. This is a feature of many vector-borne disease systems that, if understood, could guide preventative control efforts toward individuals who are expected to contribute most to pathogen transmission. We aimed to characterize factors determining biting patterns of Aedes aegypti, the principal mosquito vector of dengue virus. Methodology/Principal Findings Engorged female...

  14. Potential for dengue in South Africa: mosquito ecology with particular reference to Aedes aegypti.

    Science.gov (United States)

    Kemp, A; Jupp, P G

    1991-12-01

    Observations on prevalence, geographical distribution, utilization of artificial larval habitats and anthropophilism were made on diurnal mosquitoes at selected localities along the coast of Natal and inland in the Transvaal to identify potential vectors of dengue in South Africa. Larval collections made in artificial containers on the ground, the exposure of bamboo pots as ovitraps in trees and collection of mosquitoes biting man showed the following species as the most likely candidates for vectors: Aedes aegypti, Ae. demeilloni, Ae. simpsoni, Ae. strelitziae, Ae. furcifer, Ae. cordellieri and Eretmapodites quinquevittatus. The bamboo pots showed that Ae. aegypti and Ae. simpsoni were the most widespread species, occurring at 11 of 12 localities. Aedes aegypti was the most prevalent species with mean pot index of 60.3 +/- 9.8% (SE) and abundance index of 0.43 +/- 0.15 (SE). Aedes aegypti was frequently present as larvae in artificial containers at indices of 11-83% (mean 56.8 +/- 5.6%, SE) and was the most anthropophilic species with average biting rates of 10-29 per man-hour at 7 localities. Although Ae. aegypti was abundant in the pots at Ndumu (northern Natal) and at Skukuza (eastern Transvaal), the local populations were poorly anthropophilic at these localities. At some localities, populations of Ae. demeilloni, Ae. simpsoni and Ae. strelitziae had average biting rates of 5.4-9.6 per man-hour. Aedes furcifer was collected for the first time at Durban, extending its distribution southward to latitude 29 degrees 53' S.

  15. Wolbachia Infection Reduces Blood-Feeding Success in the Dengue Fever Mosquito, Aedes aegypti

    OpenAIRE

    Turley, Andrew P.; Moreira, Luciano A.; O'Neill, Scott L.; McGraw, Elizabeth A.

    2009-01-01

    BACKGROUND: The mosquito Aedes aegypti was recently transinfected with a life-shortening strain of the endosymbiont Wolbachia pipientis (wMelPop) as the first step in developing a biocontrol strategy for dengue virus transmission. In addition to life-shortening, the wMelPop-infected mosquitoes also exhibit increased daytime activity and metabolic rates. Here we sought to quantify the blood-feeding behaviour of Wolbachia-infected females as an indicator of any virulence or energetic drain asso...

  16. Pengaruh Frekuensi Penghisapan Darah Terhadap Perkembangan, Reproduksi,vertilitas Dan Rasio Sex Aedes Aegypti

    OpenAIRE

    Setiyaningsih, Riyani; Agustini, Maria

    2012-01-01

    Aedes aegypti is a vector of Dengue hemorrhagic fever in Indonesia. Aedesaegypti has a high reproduction ability. Each individual can produce 50-100 eggs. Which80% of them are fertile. The mosquito is multiple biting (which means each individualsucks blood several time). Based on that background, this research was aimed torecognize the frequency of blood sucking to development, reproduction, fertility, and sexratio of Ae. aegypti. Thirty Ae. aegypti mosquito were put into the plastic cupsindi...

  17. Synthesis and Larvicidal and Adult Topical Activity of Some Hydrazide-Hydrazone Derivatives Against Aedes aegypti

    Science.gov (United States)

    2014-01-01

    anticancer, antifungal, antiviral, antitumoral, antibacterial and antimalarial activities [11-13]. Recently, our group has been investigating the...ABSTRACT A series of novel hydrazide-hydrazone derivatives were synthesized and evaluated for their larvicidal and adult topical activity against...4b) showed noteworthy larvacidal activity against Aedes aegypti. Dose-response data of compound 4b showed LC50 and LC90 values of 30.5 (15.4 – 22.7

  18. Invasion of Aedes albopictus (Diptera: Culicidae) into central Africa: what consequences for emerging diseases?

    OpenAIRE

    Ngoagouni, Carine; Kamgang, Basile; Nakoun?, Emmanuel; Paupy, Chistophe; Kazanji, Mirdad

    2015-01-01

    Aedes albopictus, a mosquito native to Asia, has invaded all five continents during the past three decades. It was reported in central Africa in the 2000s, first in Cameroon, and, since then, has colonised almost all countries of the region. The species, originally considered a secondary vector of dengue viruses, has been showed to play a major role in transmission of chikungunya virus in numerous countries, including in the central African region. We review the current spread of Ae. albopict...

  19. Reducing biting rates of Aedes aegypti with metofluthrin: investigations in time and space

    OpenAIRE

    Darbro, Jonathan M.; Muzari, M. Odwell; Giblin, Arthur; Adamczyk, Rebecca M.; Ritchie, Scott A.; Devine, Gregor J.

    2017-01-01

    Background Indoor residual spraying is key to dengue control in Cairns and other parts of northern Queensland, Australia, where Aedes aegypti is prevalent, but the strategy faces challenges with regards to slow application time and, therefore, community coverage. A faster potential improvement might be the use of polyethylene netting impregnated with the volatile pyrethroid metofluthrin (SumiOne?). This formulation was assessed in rooms in three houses in Cairns, Australia. One emanator was p...

  20. A New Aedes (Finlaya) of the Niveus-Subgroup (Diptera: Culicidae)

    Science.gov (United States)

    1987-01-01

    specimens. bRange (Mode). TNTC-Too numerous to count. Table 3. Number of branches for setae of the fourth-instar larva of Aedes (Finlaya...2) 1,2(l) 1,2(2) 1,2(l) 1 2-4(2) 2,3(2) 7-ll(7) 1 1 1,2(l) aBased on counts from at least 10 setae on the holotype and 8 other specimens. bRange

  1. Ensaios sobre economia do empreendorismo

    OpenAIRE

    Gabrielito Rauter Menezes

    2015-01-01

    Esta tese é composta por três ensaios sobre Economia do Empreendedorismo. O primeiro deles trata sobre os determinantes do empreendedorismo no Brasil a partir de modelos de escolha ocupacional, usando os microdados da Pesquisa Nacional por Amostra por Domicílios (PNAD) do ano de 2012. A estratégia empírica adotada empregou os modelos de escolha discreta na estimação da escolha ocupacional. Os resultados demonstraram que existem efeitos significativos para as variáveis: anos de estudos iniciai...

  2. Reactive oxygen species production and Brugia pahangi survivorship in Aedes polynesiensis with artificial Wolbachia infection types.

    Directory of Open Access Journals (Sweden)

    Elizabeth S Andrews

    Full Text Available Heterologous transinfection with the endosymbiotic bacterium Wolbachia has been shown previously to induce pathogen interference phenotypes in mosquito hosts. Here we examine an artificially infected strain of Aedes polynesiensis, the primary vector of Wuchereria bancrofti, which is the causative agent of Lymphatic filariasis (LF throughout much of the South Pacific. Embryonic microinjection was used to transfer the wAlbB infection from Aedes albopictus into an aposymbiotic strain of Ae. polynesiensis. The resulting strain (designated "MTB" experiences a stable artificial infection with high maternal inheritance. Reciprocal crosses of MTB with naturally infected wild-type Ae. polynesiensis demonstrate strong bidirectional incompatibility. Levels of reactive oxygen species (ROS in the MTB strain differ significantly relative to that of the wild-type, indicating an impaired ability to regulate oxidative stress. Following a challenge with Brugia pahangi, the number of filarial worms achieving the infective stage is significantly reduced in MTB as compared to the naturally infected and aposymbiotic strains. Survivorship of MTB differed significantly from that of the wild-type, with an interactive effect between survivorship and blood feeding. The results demonstrate a direct correlation between decreased ROS levels and decreased survival of adult female Aedes polynesiensis. The results are discussed in relation to the interaction of Wolbachia with ROS production and antioxidant expression, iron homeostasis and the insect immune system. We discuss the potential applied use of the MTB strain for impacting Ae. polynesiensis populations and strategies for reducing LF incidence in the South Pacific.

  3. [Susceptibility of Aedes aegypti (L.) strains from Havana to a Bacillus thuringiensis var. israelensis].

    Science.gov (United States)

    Menéndez Díaz, Zulema; Rodríguez Rodríguez, Jinnay; Gato Armas, René; Companioni Ibañez, Ariamys; Díaz Pérez, Manuel; Bruzón Aguila, Rosa Yirian

    2012-01-01

    the integration of chemical and biological methods is one of the strategies for the vector control, due to the existing environmental problems and the concerns of the community as a result of the synthetic organic insecticide actions. The bacterium called Bacillus thuringiensis var. israelensis in liquid formulation has been widely used in the vector control programs in several countries and has shown high efficacy at lab in Cuba. to determine the susceptibility of Aedes aegypti collected in the municipalities of La Habana province to Bacillus thuringiensis var. israelensis. fifteen Aedes aegypti strains, one from each municipality, were used including larvae and pupas collected in 2010 and one reference strain known as Rockefeller. The aqueous formulation of Bacillus thuringiensis var. israelensis (Bactivec, Labiofam, Cuba) was used. The bioassays complied with the World Health Organization guidelines for use of bacterial larvicides in the public health sector. The larval mortality was read after 24 hours and the results were processed by the statistical system SPSS (11.0) through Probit analysis. the evaluated mosquito strains showed high susceptibility to biolarvicide, there were no significant differences in LC50 values of Ae. aegypti strains, neither in the comparison of these values with those of the reference strain. the presented results indicate that the use of Bacillus thuringiensis var. israelensis continues to be a choice for the control of Aedes aegypti larval populations in La Habana province.

  4. Wolbachia age-sex-specific density in Aedes albopictus: a host evolutionary response to cytoplasmic incompatibility?

    Directory of Open Access Journals (Sweden)

    Pablo Tortosa

    2010-03-01

    Full Text Available Wolbachia bacteria have invaded many arthropod species by inducing Cytoplasmic Incompatibility (CI. These symbionts represent fascinating objects of study for evolutionary biologists, but also powerful potential biocontrol agents. Here, we assess the density dynamics of Wolbachia infections in males and females of the mosquito Aedes albopitcus, an important vector of human pathogens, and interpret the results within an evolutionary framework.Wolbachia densities were measured in natural populations and in age controlled mosquitoes using quantitative PCR. We show that the density dynamics of the wAlbA Wolbachia strain infecting Aedes albopictus drastically differ between males and females, with a very rapid decay of infection in males only.Theory predicts that Wolbachia and its hosts should cooperate to improve the transmission of infection to offspring, because only infected eggs are protected from the effects of CI. However, incompatible matings effectively lower the fertility of infected males, so that selection acting on the host genome should tend to reduce the expression of CI in males, for example, by reducing infection density in males before sexual maturation. The rapid decay of one Wolbachia infection in Aedes albopictus males, but not in females, is consistent with this prediction. We suggest that the commonly observed reduction in CI intensity with male age reflects a similar evolutionary process. Our results also highlight the importance of monitoring infection density dynamics in both males and females to assess the efficiency of Wolbachia-based control strategies.

  5. Effect of water availability in opening containers of breeding site on Aedes aegypti life cycle

    Science.gov (United States)

    Tokachil, Najir; Yusoff, Nuraini; Saaid, Alif; Appandi, Najwa; Harun, Farhana

    2017-11-01

    The distribution of rainfall is one of the factors which contribute to the development of Aedes aegypti life cycle. The fluctuation of rainfall might influence the acceleration of Aedes aegypti growth by providing sufficient breeding sites. In this research, the availability of water in an opening container of the breeding site is considered as a significant variable which affects the distinct stages structure in mosquito life cycle which egg, larva, pupa, and adult. A stage-structured Lefkovitch matrix model was used by considering the quantity of water contains in an opening container and life cycle of Aedes aegypti. The maximum depth of water in the container was also taken into account in order to find the time duration of mosquito life cycle to complete. We found that the maximum depth of water availability in mosquito breeding site influenced the abundance of the mosquito population. Hence, the containers are filled with sufficient water be able to stand from hot temperature for several days before drying out might continue to provide mosquito breeding site. In the future, it is recommended to consider other factors which affect the quantity of water in mosquito breeding sites such as heavy rain and wind blows.

  6. Local extinction of the Asian tiger mosquito (Aedes albopictus) following rat eradication on Palmyra Atol

    Science.gov (United States)

    Lafferty, Kevin D.; McLaughlin, John P.; Gruner, Daniel S.; Bogar, Taylor A.; Bui, An; Childress, Jasmine N.; Espinoza, Magaly; Forbes, Elizabeth S.; Johnston, Cora A.; Klope, Maggie; Kuile, Ana Miller-ter; Lee, Michelle; Plummer, Katherine A.; Weber, David A.; Young, Ronald T.; Young, Hillary S.

    2018-01-01

    The Asian tiger mosquito, Aedes albopictus, appears to have been extirpated from Palmyra Atoll following rat eradication. Anecdotal biting reports, collection records, and regular captures in black-light traps showed the species was present before rat eradication. Since then, there have been no biting reports and no captures over 2 years of extensive trapping (black-light and scent traps). By contrast, the southern house mosquito, Culex quinquefasciatus, was abundant before and after rat eradication. We hypothesize that mammals were a substantial and preferred blood meal for Aedes, whereas Culex feeds mostly on seabirds. Therefore, after rat eradication, humans and seabirds alone could not support positive population growth or maintenance of Aedes. This seems to be the first documented accidental secondary extinction of a mosquito. Furthermore, it suggests that preferred host abundance can limit mosquito populations, opening new directions for controlling important disease vectors that depend on introduced species like rats.

  7. Adulticidal Susceptibility Evaluation of Aedes albopictus Using New Diagnostic Doses in Penang Island, Malaysia.

    Science.gov (United States)

    Rahim, Junaid; Ahmad, Abu H; Ahmad, Hamdan; Ishak, Intan H; Rus, Adanan Che; Maimusa, Hamisu A

    2017-09-01

    Insecticide-based vector control approaches are facing challenges due to the development of resistance in vector mosquitoes. Therefore, a proper resistance surveillance program using baseline lethal concentrations is crucial for resistance management strategies. Currently, the World Health Organization's (WHO) diagnostic doses established for Aedes aegypti and Anopheles species are being used to study the resistance status of Aedes albopictus. In this study, we established the diagnostic doses for permethrin, deltamethrin, and malathion using a known susceptible reference strain. Five field-collected populations were screened against these doses, following the WHO protocol. This study established the diagnostic dose of malathion at 2.4%, permethrin at 0.95%, and deltamethrin at 0.28%, which differ from the WHO doses for Aedes aegypti and Anopheles spp. Among the insecticides tested on the 5 wild populations, only deltamethrin showed high effectiveness. Different susceptibility and resistance patterns were observed with permethrin, malathion, and dichloro-diphenyl-trichloroethane (DDT) at 4%. This study may assist the health authorities to improve future chemical-based vector control operations in dengue-endemic areas.

  8. In silico models for predicting vector control chemicals targeting Aedes aegypti

    Science.gov (United States)

    Devillers, J.; Lagneau, C.; Lattes, A.; Garrigues, J.C.; Clémenté, M.M.; Yébakima, A.

    2014-01-01

    Human arboviral diseases have emerged or re-emerged in numerous countries worldwide due to a number of factors including the lack of progress in vaccine development, lack of drugs, insecticide resistance in mosquitoes, climate changes, societal behaviours, and economical constraints. Thus, Aedes aegypti is the main vector of the yellow fever and dengue fever flaviviruses and is also responsible for several recent outbreaks of the chikungunya alphavirus. As for the other mosquito species, the A. aegypti control relies heavily on the use of insecticides. However, because of increasing resistance to the different families of insecticides, reduction of Aedes populations is becoming increasingly difficult. Despite the unquestionable utility of insecticides in fighting mosquito populations, there are very few new insecticides developed and commercialized for vector control. This is because the high cost of the discovery of an insecticide is not counterbalanced by the ‘low profitability’ of the vector control market. Fortunately, the use of quantitative structure–activity relationship (QSAR) modelling allows the reduction of time and cost in the discovery of new chemical structures potentially active against mosquitoes. In this context, the goal of the present study was to review all the existing QSAR models on A. aegypti. The homology and pharmacophore models were also reviewed. Specific attention was paid to show the variety of targets investigated in Aedes in relation to the physiology and ecology of the mosquito as well as the diversity of the chemical structures which have been proposed, encompassing man-made and natural substances. PMID:25275884

  9. Efficacy of alpha-cypermethrin and lambda-cyhalothrin applications to prevent Aedes breeding in tires.

    Science.gov (United States)

    Pettit, William J; Whelan, Peter I; McDonnell, Joseph; Jacups, Susan P

    2010-12-01

    The efficacy of alpha-cypermethrin (Cyperthor) and lambda-cyhalothrin (Demand) to prevent mosquito larval colonization of water-containing receptacles was investigated using 2 differing applications in disused car tires in Darwin, Australia. Insecticide treatments were applied uniformly to the inside surfaces of 2 categories of tires: 1) dry tires that were partially filled with water 24 h after spraying and 2) wet tires partially filled with water prior to spraying. All mosquito larvae, pupae, and dead adults were collected from the treatment and control tires weekly over the 24-wk study period and were later identified to species in the laboratory. Control tires were colonized by Aedes notoscriptus in wk 2 and by Culex quinquefasciatus in wk 4. Aedes notoscriptus failed to colonize any alpha-cypermethrin-treated tires until wk 22 and did not colonize any lambda-cyhalothrin-treated tires during the 24-wk trial. Culex quinquefasciatus colonized alpha-cypermethrin and lambda-cyhalothrin-treated tires from wk 11 and wk 15, respectively. These results indicate both insecticides using either application method can prevent colonization of Ae. notoscriptus for at least 20 wk and demonstrate great potential for the prevention of breeding in receptacles for other receptacle-breeding Aedes species, such as the dengue vectors, Ae. aegypti and Ae. albopictus.

  10. Detecção de Aedes aegypti e Aedes albopictus, na zona urbana do município de Catanduva-SP, após controle de epidemia de dengue

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    Rubens Pinto Cardoso Junior

    1997-02-01

    Full Text Available Após a realização dos trabalhos de controle visando à interrupção da transmissão do vírus do dengue, iniciou-se um trabalho de monitorização de Aedes aegypti e Aedes albopictus com dois métodos de vigilância entomológica: Índice de Breteau (IB e ovitrampas. Pretendeu-se avaliar o tempo necessário para que as espécies envolvidas fossem novamente detectadas na área urbana do município de Catanduva, SP. As ovitrampas apresentaram positividade para Aedes aegypti dois meses após os trabalhos de controle, enquanto o Índice de Breteau veio a positivar-se somente no quarto mês após o término dos referidos trabalhos.After the realization of control research that had in view the transmition of dengue virus, we started to monitor two kinds of entomological vigilance, Breteau Index and ovitrap. We intended to evaluate the necessary time elapsed before Aedes sp mosquitoes were again detected at the urban area of Catanduva s town (SP. The ovitraps showed positiviness for the Aedes aegypti two months after the control research, while the Breteau Index became positive only at the fourth month after the end of the refered research.

  11. Larval development of Aedes aegypti and Aedes albopictus in peri-urban brackish water and its implications for transmission of arboviral diseases.

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    Ranjan Ramasamy

    2011-11-01

    Full Text Available Aedes aegypti (Linnaeus and Aedes albopictus Skuse mosquitoes transmit serious human arboviral diseases including yellow fever, dengue and chikungunya in many tropical and sub-tropical countries. Females of the two species have adapted to undergo preimaginal development in natural or artificial collections of freshwater near human habitations and feed on human blood. While there is an effective vaccine against yellow fever, the control of dengue and chikungunya is mainly dependent on reducing freshwater preimaginal development habitats of the two vectors. We show here that Ae. aegypti and Ae. albopictus lay eggs and their larvae survive to emerge as adults in brackish water (water with 30 ppt salt are termed fresh, brackish and saline respectively. Brackish water with salinity of 2 to 15 ppt in discarded plastic and glass containers, abandoned fishing boats and unused wells in coastal peri-urban environment were found to contain Ae. aegypti and Ae. albopictus larvae. Relatively high incidence of dengue in Jaffna city, Sri Lanka was observed in the vicinity of brackish water habitats containing Ae. aegypti larvae. These observations raise the possibility that brackish water-adapted Ae. aegypti and Ae. albopictus may play a hitherto unrecognized role in transmitting dengue, chikungunya and yellow fever in coastal urban areas. National and international health authorities therefore need to take the findings into consideration and extend their vector control efforts, which are presently focused on urban freshwater habitats, to include brackish water larval development habitats.

  12. Bromeliad-inhabiting mosquitoes in an urban botanical garden of dengue endemic Rio de Janeiro. Are bromeliads productive habitats for the invasive vectors Aedes aegypti and Aedes albopictus?

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    Mocellin, Márcio Goulart; Simões, Taynãna César; do Nascimento, Teresa Fernandes Silva; Teixeira, Maria Lucia França; Lounibos, Leon Philip; de Oliveira, Ricardo Lourenço

    2012-01-01

    Immatures of both Aedes aegypti and Aedes albopictus have been found in water-holding bromeliad axils in Brazil. Removal of these plants or their treatment with insecticides in public and private gardens have been undertaken during dengue outbreaks in Brazil despite uncertainty as to their importance as productive habitats for dengue vectors. From March 2005-February 2006, we sampled 120 randomly selected bromeliads belonging to 10 species in a public garden less than 200 m from houses in a dengue-endemic neighborhood in Rio de Janeiro. A total of 2,816 mosquito larvae and pupae was collected, with an average of 5.87 immatures per plant per collection. Culex (Microculex) pleuristriatus and Culex spp of the Ocellatus Group were the most abundant culicid species, found in all species of bromeliads; next in relative abundance were species of the genus Wyeomyia. Only two individuals of Ae. aegypti (0.07%) and five of Ae. albopictus (0.18%) were collected from bromeliads. By contrast, immatures of Ae. aegypti were found in manmade containers in nearly 5% of nearby houses. These results demonstrate that bromeliads are not important producers of Ae. aegypti and Ae. albopictus and, hence, should not be a focus for dengue control. However, the results of this study of only one year in a single area may not represent outcomes in other urban localities where bromeliads, Ae. aegypti and dengue coincide in more disturbed habitats. PMID:20140379

  13. Host-feeding pattern of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in heterogeneous landscapes of South Andaman, Andaman and Nicobar Islands, India.

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    Sivan, Arun; Shriram, A N; Sunish, I P; Vidhya, P T

    2015-09-01

    Mosquito foraging behavior is a determinant of host-vector contact and has an impact on the risk of arboviral epidemics. Therefore, blood-feeding patterns is a useful tool for assessing the role in pathogen transmission by vector mosquitoes. Competent vectors of dengue and chikungunya viz. Aedes aegypti and Aedes albopictus are widely prevalent in the Andaman and Nicobar archipelago. Considering the vector potential, medical importance of both these mosquito species and lack of information on host-feeding patterns, blood meal analysis of both these vector mosquitoes was undertaken. Biogents Sentinel traps were used for sampling blooded mosquitoes, for identifying the source of blood meal by agar gel-precipitin test. We identified vertebrate source of 147 and 104 blood meals in Ae. aegypti and Ae. albopictus from heterogeneous landscapes in South Andaman district. Results revealed that Ae. aegypti (88 %) and Ae. albopictus (49 %) fed on human and a small proportion on mammals and fowls, indicative of predominance of anthropophilism. Ae. aegypti predominantly fed on human blood (94.2 %-densely built urban, 89.8 %-low vegetation coverage, and 78.3 %-medium vegetation coverage). Anthropophilism in Ae. albopictus was maximal in densely built urban (90.5 %) and progressively decreased from low vegetation-vegetation/forested continuum (66.7, 36.4, and 8.7 %), indicating plasticity in feeding across these landscapes. Epidemiological significance of the findings is discussed.

  14. Dispersal of Aedes aegypti and Aedes albopictus (Diptera: Culicidae in an urban endemic dengue area in the State of Rio de Janeiro, Brazil

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    Nildimar Alves Honório

    2003-03-01

    Full Text Available Experimental releases of female Aedes (Stegomyia aegypti and Aedes (Stegomyia albopictus were performed in August and September 1999, in an urban area of Nova Iguaçu, State of Rio de Janeiro, Brazil, to estimate their flight range in a circular area of 1,600 m where 1,472 ovitraps were set. Releases of 3,055 Ae. aegypti and 2,225 Ae. albopictus females, fed with rubidium (Rb-marked blood and surgically prevented from subsequent blood-feeding, were separated by 11 days. Rb was detected in ovitrap-collected eggs by atomic emission spectrophotometry. Rb-marked eggs of both species were detected up to 800 m from the release point. Eggs of Ae. albopictus were more numerous and more heterogeneously distributed in the area than those of Ae. aegypti. Eggs positively marked for Rb were found at all borders of the study area, suggesting that egg laying also occurred beyond these limits. Results from this study suggest that females can fly at least 800 m in 6 days and, if infected, potentially spread virus rapidly.

  15. Evaluating the Vector Control Potential of the In2Care® Mosquito Trap Against Aedes aegypti and Aedes albopictus Under Semifield Conditions in Manatee County, Florida.

    Science.gov (United States)

    Buckner, Eva A; Williams, Katie F; Marsicano, Ambyr L; Latham, Mark D; Lesser, Christopher R

    2017-09-01

    Successful integrated vector management programs may need new strategies in addition to conventional larviciding and adulticiding strategies to target Aedes aegypti and Ae. albopictus, which can develop in small, often cryptic, artificial and natural containers. The In2Care® mosquito trap was recently developed to target and kill larval and adult stages of these invasive container-inhabiting Aedes mosquitoes by utilizing autodissemination. Gravid females that visit the trap pick up pyriproxyfen (PPF) that they later transfer to nearby larval habitats as well as Beauveria bassiana spores that slowly kill them. We assessed the efficacy of the In2Care mosquito trap in a semifield setting against locally sourced strains of Ae. aegypti and Ae. albopictus. We found that the In2Care mosquito trap is attractive to gravid Ae. aegypti and Ae. albopictus females and serves as an egg sink, preventing any adult emergence from the trap (P = 0.0053 for both species). Adult females successfully autodisseminated PPF to surrounding water-filled containers, leading to a statistically significant reduction in new mosquito emergence (P ≤ 0.0002 for both species). Additionally, we found effective contamination with Beauveria bassiana spores, which significantly reduced the survivorship of exposed Ae. aegypti and Ae. albopictus (P ≤ 0.008 for both species in all experimental setups). In summary, the In2Care mosquito trap successfully killed multiple life stages of 2 main mosquito vector species found in Florida under semifield conditions.

  16. The Climate Range Expansion of Aedes albopictus (Diptera: Culicidae) in Asia Inferred From the Distribution of Albopictus Subgroup Species of Aedes (Stegomyia).

    Science.gov (United States)

    Mogi, M; Armbruster, P A; Tuno, N; Aranda, C; Yong, H S

    2017-11-07

    We compared climatic distribution ranges between Aedes albopictus (Skuse) (Diptera: Culicidae) and the five wild (nondomesticated) species of Albopictus Subgroup of Scutellaris Group of Aedes (Stegomyia) in southern Asia. Distribution sites of the wild species concentrate in seasonal forest and savannah climate zones in India, Indochina, and southern China. The distribution of Ae. albopictus is broader than the wild species under 1) tropical rain-forest climate, 2) steppe and temperate savannah climate, and 3) continental climate with large seasonal temperature variation (hot summer and cold winter) at temperate lowlands (northernmost sites 40°N in Ae. albopictus vs 32°N in the wild species). However, the distribution of Ae. albopictus is more limited at tropical and subtropical highlands where the climate is cool but less continental (small seasonal variation, mild summer, and winter). We discuss a possibility that the broader climate ranges of Ae. albopictus are ecological or eco-evolutionary consequences of adaptation to human habitats. We also propose a general scenario for the origin, dispersal, and adaptation of Ae. albopictus in Asia as a hypothesis for future research. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  17. Potencial de Bacillus thuringiensis israelensis Berliner no controle de Aedes aegypti Potential of Bacillus thuringiensis israelensis Berliner for controlling Aedes aegypti

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    Ricardo Antonio Polanczyk

    2003-12-01

    Full Text Available Relata-se a importância da bactéria entomopatogênica Bacillus thuringiensis israelensis para o controle de Aedes aegypti. São abordados a utilização e potencial de B. thuringiensis israelensis contra o mosquito vetor da dengue. Outros aspectos são discutidos como a evolução da resistência dos insetos em relação aos inseticidas químicos e as vantagens e desvantagens do controle microbiano como estratégia de controle. É dada ênfase à importância da utilização desta bactéria no Brasil como alternativa para resolver o problema em questão sem afetar o ambiente, o homem e outros vertebrados nas áreas de risco.The importance of the entomopathogenic bacterium Bacillus thuringiensis israelensis in the control of Aedes aegypti is presented. The use and potential of B. thuringiensis israelensis against the mosquito vector of dengue fever is described. Other aspects such as insect's resistance development against chemicals and advantages and constraints of using microbial control are discussed. Emphasis is given to the importance of the use of this bacterium in Brazil, which could contribute significantly to solving the mosquito problem without affecting the environment, humans and others invertebrate organisms in critical regions.

  18. Co-breeding Association of Aedes albopictus (Skuse) and Aedes aegypti (Linnaeus) (Diptera: Culicidae) in Relation to Location and Container Size.

    Science.gov (United States)

    Hashim, Nur Aida; Ahmad, Abu Hassan; Talib, Anita; Athaillah, Farida; Krishnan, Kumara Thevan

    2018-03-01

    The occurrence of major outbreaks of dengue, and other vector borne diseases such as chikungunya and zika in tropical and subtropical regions has rendered control of the diseases a top-priority for many affected countries including Malaysia. Control of the mosquito vectors Aedes aegypti and Aedes albopictus through the reduction of breeding sites and the application of insecticides to kill immature forms and adults are the main control efforts to combat these diseases. The present study describes the association between Ae. albopictus and Ae. aegypti in shared breeding sites. This study is important given that any measure taken against one species may affect the other. A yearlong larval survey was conducted in four dengue endemic areas of Penang Island. Sorenson's coefficient index indicated that no association between number of the immatures of the two species regardless of container size and study location. Therefore, the mean number Ae. albopictus immature was not decreased in the presence of Ae. aegypti in shared breeding container. However Ae. aegypti appeared to prefer breeding in habitats not occupied by Ae. albopictus , the two species sharing breeding sites only where available containers were limited. In control efforts, eliminating the preferred breeding containers for one species might not affect or reduce the population of the other species.

  19. Bromeliad-inhabiting mosquitoes in an urban botanical garden of dengue endemic Rio de Janeiro - Are bromeliads productive habitats for the invasive vectors Aedes aegypti and Aedes albopictus?

    Directory of Open Access Journals (Sweden)

    Márcio Goulart Mocellin

    2009-12-01

    Full Text Available Immatures of both Aedes aegypti and Aedes albopictus have been found in water-holding bromeliad axils in Brazil. Removal of these plants or their treatment with insecticides in public and private gardens have been undertaken during dengue outbreaks in Brazil despite uncertainty as to their importance as productive habitats for dengue vectors. From March 2005-February 2006, we sampled 120 randomly selected bromeliads belonging to 10 species in a public garden less than 200 m from houses in a dengue-endemic neighborhood in Rio de Janeiro. A total of 2,816 mosquito larvae and pupae was collected, with an average of 5.87 immatures per plant per collection. Culex (Microculex pleuristriatus and Culex spp of the Ocellatus Group were the most abundant culicid species, found in all species of bromeliads; next in relative abundance were species of the genus Wyeomyia. Only two individuals of Ae. aegypti (0.07% and five of Ae. albopictus(0.18% were collected from bromeliads. By contrast, immatures of Ae. aegypti were found in manmade containers in nearly 5% of nearby houses. These results demonstrate that bromeliads are not important producers of Ae. aegypti and Ae. albopictus and, hence, should not be a focus for dengue control. However, the results of this study of only one year in a single area may not represent outcomes in other urban localities where bromeliads, Ae. aegypti and dengue coincide in more disturbed habitats.

  20. Stochastic modeling and simulation of reaction-diffusion system with Hill function dynamics.

    Science.gov (United States)

    Chen, Minghan; Li, Fei; Wang, Shuo; Cao, Young

    2017-03-14

    Stochastic simulation of reaction-diffusion systems presents great challenges for spatiotemporal biological modeling and simulation. One widely used framework for stochastic simulation of reaction-diffusion systems is reaction diffusion master equation (RDME). Previous studies have discovered that for the RDME, when discretization size approaches zero, reaction time for bimolecular reactions in high dimensional domains tends to infinity. In this paper, we demonstrate that in the 1D domain, highly nonlinear reaction dynamics given by Hill function may also have dramatic change when discretization size is smaller than a critical value. Moreover, we discuss methods to avoid this problem: smoothing over space, fixed length smoothing over space and a hybrid method. Our analysis reveals that the switch-like Hill dynamics reduces to a linear function of discretization size when the discretization size is small enough. The three proposed methods could correctly (under certain precision) simulate Hill function dynamics in the microscopic RDME system.