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Sample records for highly altered rivers

  1. Assessing the hydrologic alteration of the Yangtze River using the histogram matching approach

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    Huang, F.; Zhang, N.; Guo, L. D.; Xia, Z. Q.

    2016-08-01

    Hydrologic changes of the Yangtze River, an important river with abundant water resources in China, were investigated using the Histogram Matching Approach. Daily streamflow data spanning the time interval from 1955 to 2013 was collected from Yichang and Datong stations, which monitor the hydrologic processes of the upper and lower reach of the Yangtze River, respectively. The Gezhouba Dam, the first dam constructed at the main stream of the Yangtze River, started operations in 1981. 1981 was used to differentiate the pre-dam (1955-1980) and post-dam (1981-2013) hydrologic regimes. The hydrologic regime was quantified by the Indicators of Hydrologic Alteration. The overall alteration degree of the upper Yangtze River was 31% and the alteration degree of every hydrologic indicator ranged from 10% to 81%. Only 1, 5 and 26 hydrologic indicators were altered at high, moderate and low degrees, respectively. The overall alteration degree of the lower Yangtze River was 30%, and the alteration degree of every hydrologic indicator ranged from 8% to 49%. No high alteration degree was detected at the Datong station. Ten hydrologic indicators were altered at moderate degrees and 22 hydrologic indicators were altered at low degrees. Significant increases could be observed for the low-flow relevant indicators, including the monthly flow from January-March, the annual minimum 1, 3, 7, 30 and 90-day flows, and the base flow index.

  2. Hydrologic alteration affects aquatic plant assemblages in an arid-land river

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    Vinson, Mark; Hestmark, Bennett; Barkworth, Mary E.

    2014-01-01

    We evaluated the effects of long-term flow alteration on primary-producer assemblages. In 1962, Flaming Gorge Dam was constructed on the Green River. The Yampa River has remained an unregulated hydrologically variable river that joins the Green River 100 km downstream from Flaming Gorge Dam. In the 1960s before dam construction only sparse occurrences of two macroalgae, Cladophora and Chara, and no submerged vascular plants were recorded in the Green and Yampa rivers. In 2009–2010, aquatic plants were abundant and widespread in the Green River from the dam downstream to the confluence with the Yampa River. The assemblage consisted of six vascular species, Elodea canadensis, Myriophyllum sibiricum, Nasturtium officinale,Potamogeton crispus, Potamogeton pectinatus, and Ranunculus aquatilis, the macroalgae Chara and Cladophora, and the bryophyte, Amblystegium riparium. In the Green River downstream from the Yampa River, and in the Yampa River, only sparse patches of Chara and Cladophora growing in the splash zone on boulders were collected. We attribute the observed changes in the Green River to an increase in water transparency and a reduction in suspended and bed-load sediment and high flow disturbances. The lack of hydrophyte colonization downstream from the confluence with the Yampa River has implications for understanding tributary amelioration of dam effects and for designing more natural flow-regime schedules downstream from large dams.

  3. Assessing flow regime alterations in a temporary river – the River Celone case study

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    De Girolamo Anna Maria

    2015-09-01

    Full Text Available In this paper, we present an approach to evaluate the hydrological alterations of a temporary river. In these rivers, it is expected that anthropogenic pressures largely modify low-flow components of the flow regime with consequences for aquatic habitat and diversity in invertebrate species. First, by using a simple hydrological index (IARI river segments of the Celone stream (southern Italy whose hydrological regime is significantly influenced by anthropogenic activities have been identified. Hydrological alteration has been further classified through the analysis of two metrics: the degree (Mf and the predictability of dry flow conditions (Sd6. Measured streamflow data were used to calculate the metrics in present conditions (impacted. Given the lack of data from pristine conditions, simulated streamflow time series were used to calculate the metrics in reference conditions. The Soil and Water Assessment Tool (SWAT model was used to estimate daily natural streamflow. Hydrological alterations associated with water abstractions, point discharges and the presence of a reservoir were assessed by comparing the metrics (Mf, Sd6 before and after the impacts. The results show that the hydrological regime of the river segment located in the upper part of the basin is slightly altered, while the regime of the river segment downstream of the reservoir is heavily altered. This approach is intended for use with ecological metrics in defining the water quality status and in planning streamflow management activities.

  4. Field guide to hydrothermal alteration in the White River altered area and in the Osceola Mudflow, Washington

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    John, David A.; Rytuba, James J.; Ashley, Roger P.; Blakely, Richard J.; Vallance, James W.; Newport, Grant R.; Heinemeyer, Gary R.

    2003-01-01

    . The morning of the trip will examine the White River altered area, which includes high-level alteration related to a large, early Miocene magmatic-hydrothermal system exposed about 10 km east of Enumclaw, Washington. Here, vuggy silica alteration is being quarried for silica and advanced argillic alteration has been prospected for alunite. Clay-filled fractures and sulfide-rich, fine-grained sedimentary rocks of hydrothermal origin locally are enriched in precious metals. Many hydrothermal features common in high-sulfidation gold-silver deposits and in advanced argillic alteration zones overlying porphyry copper deposits (for example, Gustafson and Hunt, 1975; Hedenquist and others, 2000; Sillitoe, 2000) are exposed, although no economic base or precious metal mineralized rock has been discovered to date. The afternoon will be spent examining two exposures of the Osceola Mudflow along the White River. The Osceola Mudflow contains abundant clasts of altered Quaternary rocks from Mount Rainier that show various types of hydrothermal alteration and hydrothermal features. The mudflow matrix contains abundant hydrothermal clay minerals that added cohesiveness to the debris flow and helped allow it to travel much farther down valley than other, noncohesive debris flows from Mount Rainier (Crandell, 1971; Vallance and Scott, 1997). The White River altered area is the subject of ongoing studies by geoscientists from Weyerhaeuser Company and the U.S. Geological Survey (USGS). The generalized descriptions of the geology, geophysics, alteration, and mineralization presented here represent the preliminary results of this study (Ashley and others, 2003). Additional field, geochemical, geochronologic, and geophysical studies are underway. The Osceola Mudflow and other Holocene debris flows from Mount Rainier also are the subject of ongoing studies by the USGS (for example, Breit and others, 2003; John and others, 2003; Plumlee and others, 2003, Sisson and others, 2003; Vallance and

  5. Evaluation of Spatial Pattern of Altered Flow Regimes on a River Network Using a Distributed Hydrological Model

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    Ryo, Masahiro; Iwasaki, Yuichi; Yoshimura, Chihiro; Saavedra V., Oliver C.

    2015-01-01

    Alteration of the spatial variability of natural flow regimes has been less studied than that of the temporal variability, despite its ecological importance for river ecosystems. Here, we aimed to quantify the spatial patterns of flow regime alterations along a river network in the Sagami River, Japan, by estimating river discharge under natural and altered flow conditions. We used a distributed hydrological model, which simulates hydrological processes spatiotemporally, to estimate 20-year daily river discharge along the river network. Then, 33 hydrologic indices (i.e., Indicators of Hydrologic Alteration) were calculated from the simulated discharge to estimate the spatial patterns of their alterations. Some hydrologic indices were relatively well estimated such as the magnitude and timing of maximum flows, monthly median flows, and the frequency of low and high flow pulses. The accuracy was evaluated with correlation analysis (r > 0.4) and the Kolmogorov–Smirnov test (α = 0.05) by comparing these indices calculated from both observed and simulated discharge. The spatial patterns of the flow regime alterations varied depending on the hydrologic indices. For example, both the median flow in August and the frequency of high flow pulses were reduced by the maximum of approximately 70%, but these strongest alterations were detected at different locations (i.e., on the mainstream and the tributary, respectively). These results are likely caused by different operational purposes of multiple water control facilities. The results imply that the evaluation only at discharge gauges is insufficient to capture the alteration of the flow regime. Our findings clearly emphasize the importance of evaluating the spatial pattern of flow regime alteration on a river network where its discharge is affected by multiple water control facilities. PMID:26207997

  6. Evaluation of Spatial Pattern of Altered Flow Regimes on a River Network Using a Distributed Hydrological Model.

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    Masahiro Ryo

    Full Text Available Alteration of the spatial variability of natural flow regimes has been less studied than that of the temporal variability, despite its ecological importance for river ecosystems. Here, we aimed to quantify the spatial patterns of flow regime alterations along a river network in the Sagami River, Japan, by estimating river discharge under natural and altered flow conditions. We used a distributed hydrological model, which simulates hydrological processes spatiotemporally, to estimate 20-year daily river discharge along the river network. Then, 33 hydrologic indices (i.e., Indicators of Hydrologic Alteration were calculated from the simulated discharge to estimate the spatial patterns of their alterations. Some hydrologic indices were relatively well estimated such as the magnitude and timing of maximum flows, monthly median flows, and the frequency of low and high flow pulses. The accuracy was evaluated with correlation analysis (r > 0.4 and the Kolmogorov-Smirnov test (α = 0.05 by comparing these indices calculated from both observed and simulated discharge. The spatial patterns of the flow regime alterations varied depending on the hydrologic indices. For example, both the median flow in August and the frequency of high flow pulses were reduced by the maximum of approximately 70%, but these strongest alterations were detected at different locations (i.e., on the mainstream and the tributary, respectively. These results are likely caused by different operational purposes of multiple water control facilities. The results imply that the evaluation only at discharge gauges is insufficient to capture the alteration of the flow regime. Our findings clearly emphasize the importance of evaluating the spatial pattern of flow regime alteration on a river network where its discharge is affected by multiple water control facilities.

  7. Evaluation of Spatial Pattern of Altered Flow Regimes on a River Network Using a Distributed Hydrological Model.

    Science.gov (United States)

    Ryo, Masahiro; Iwasaki, Yuichi; Yoshimura, Chihiro; Saavedra V, Oliver C

    2015-01-01

    Alteration of the spatial variability of natural flow regimes has been less studied than that of the temporal variability, despite its ecological importance for river ecosystems. Here, we aimed to quantify the spatial patterns of flow regime alterations along a river network in the Sagami River, Japan, by estimating river discharge under natural and altered flow conditions. We used a distributed hydrological model, which simulates hydrological processes spatiotemporally, to estimate 20-year daily river discharge along the river network. Then, 33 hydrologic indices (i.e., Indicators of Hydrologic Alteration) were calculated from the simulated discharge to estimate the spatial patterns of their alterations. Some hydrologic indices were relatively well estimated such as the magnitude and timing of maximum flows, monthly median flows, and the frequency of low and high flow pulses. The accuracy was evaluated with correlation analysis (r > 0.4) and the Kolmogorov-Smirnov test (α = 0.05) by comparing these indices calculated from both observed and simulated discharge. The spatial patterns of the flow regime alterations varied depending on the hydrologic indices. For example, both the median flow in August and the frequency of high flow pulses were reduced by the maximum of approximately 70%, but these strongest alterations were detected at different locations (i.e., on the mainstream and the tributary, respectively). These results are likely caused by different operational purposes of multiple water control facilities. The results imply that the evaluation only at discharge gauges is insufficient to capture the alteration of the flow regime. Our findings clearly emphasize the importance of evaluating the spatial pattern of flow regime alteration on a river network where its discharge is affected by multiple water control facilities.

  8. Impact of the Mekong River flow alteration on the Tonle Sap flood pulse.

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    Kummu, Matti; Sarkkula, Juha

    2008-05-01

    Rapid development in the upper reaches of the Mekong River, in the form of construction of large hydropower dams and reservoirs, large irrigation schemes, and rapid urban development, is putting water resources under stress. Recent studies have concluded that these developments will lead to flow alterations in the Mekong River. These flow alterations would threaten the sensitive ecosystems downstream, particularly Tonle Sap River, Tonle Sap Lake, its floodplain, and its gallery forest and protected areas, by changing the flood-pulse system of the lake. This article estimates the changes in parameters of the Tonle Sap flood pulse due to the aforementioned flow alterations. The impacts on the flooded area and loss of gallery forest and protected areas were analyzed using geographic information system-based methods. Relatively small rises in the dry-season lake water level would permanently inundate disproportionately large areas of floodplain, rendering it inaccessible to floodplain vegetation and eroding the productivity basis of the ecosystem. It is highly important to maintain the natural hydrological pattern of the Mekong River, particularly the dry-season water levels, to preserve Tonle Sap Lake's ecosystem productivity.

  9. Effect of Hydrologic Alteration on the Community Succession of Macrophytes at Xiangyang Site, Hanjiang River, China

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    Na Yang

    2017-01-01

    Full Text Available With the intensification of human activities over the past three decades in China, adverse effects on river ecosystem become more serious especially in the Hanjiang River. Xiangyang site is an important spawn ground for four domestic fishes in the downstream region of Hanjiang River. Based on the field survey results of macrophytes during 1997–2000 and 2013-2014, community succession of aquatic macrophytes at Xiangyang site was evaluated and discussed. Two-key ecologic-related hydrologic characteristics, flow regime and water level, were identified as the main influence factors. The EFC (environmental flow components parameters were adopted to evaluate the alteration of flow regimes at Xiangyang site during 1941–2013. Evaluation results demonstrate a highly altered flow process after being regulated by reservoir. The flow patterns tend to be an attenuation process with no large floods occurring but a higher monthly low flow. Furthermore, the water level decreased and fluctuation reduced after the dam was built, which caused the decrease of biomass but favored the submerged macrophytes during 1995–2009. However, with the water level increasing after 2010 and gently fluctuating, due to uplift by the hydraulic projects downstream as well as the flow attenuation, the dominant position of submerged macrophytes will be weakened.

  10. Effect of Hydrologic Alteration on the Community Succession of Macrophytes at Xiangyang Site, Hanjiang River, China

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    Duan, Kai

    2017-01-01

    With the intensification of human activities over the past three decades in China, adverse effects on river ecosystem become more serious especially in the Hanjiang River. Xiangyang site is an important spawn ground for four domestic fishes in the downstream region of Hanjiang River. Based on the field survey results of macrophytes during 1997–2000 and 2013-2014, community succession of aquatic macrophytes at Xiangyang site was evaluated and discussed. Two-key ecologic-related hydrologic characteristics, flow regime and water level, were identified as the main influence factors. The EFC (environmental flow components) parameters were adopted to evaluate the alteration of flow regimes at Xiangyang site during 1941–2013. Evaluation results demonstrate a highly altered flow process after being regulated by reservoir. The flow patterns tend to be an attenuation process with no large floods occurring but a higher monthly low flow. Furthermore, the water level decreased and fluctuation reduced after the dam was built, which caused the decrease of biomass but favored the submerged macrophytes during 1995–2009. However, with the water level increasing after 2010 and gently fluctuating, due to uplift by the hydraulic projects downstream as well as the flow attenuation, the dominant position of submerged macrophytes will be weakened. PMID:28127493

  11. Quantifying habitat benefits of channel reconfigurations on a highly regulated river system, Lower Missouri River, USA

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    Erwin, Susannah O.; Jacobson, Robert B.; Elliott, Caroline M.

    2017-01-01

    We present a quantitative analysis of habitat availability in a highly regulated lowland river, comparing a restored reach with two reference reaches: an un-restored, channelized reach, and a least-altered reach. We evaluate the effects of channel modifications in terms of distributions of depth and velocity as well as distributions and availability of habitats thought to be supportive of an endangered fish, the pallid sturgeon (Scaphirhynchus albus). It has been hypothesized that hydraulic conditions that support food production and foraging may limit growth and survival of juvenile pallid sturgeon. To evaluate conditions that support these habitats, we constructed two-dimensional hydrodynamic models for the three study reaches, two located in the Lower Missouri River (channelized and restored reaches) and one in the Yellowstone River (least-altered reach). Comparability among the reaches was improved by scaling by bankfull discharge and bankfull channel area. The analysis shows that construction of side-channel chutes and increased floodplain connectivity increase the availability of foraging habitat, resulting in a system that is more similar to the reference reach on the Yellowstone River. The availability of food-producing habitat is low in all reaches at flows less than bankfull, but the two reaches in the Lower Missouri River – channelized and restored – display a threshold-like response as flows overtop channel banks, reflecting the persistent effects of channelization on hydraulics in the main channel. These high lateral gradients result in punctuated ecological events corresponding to flows in excess of bankfull discharge. This threshold effect in the restored reach remains distinct from that of the least-altered reference reach, where hydraulic changes are less abrupt and overbank flows more gradually inundate the adjacent floodplain. The habitat curves observed in the reference reach on the Yellowstone River may not be attainable within the

  12. Historical Maps Potential on the Assessment of the Hydromorphological Changes in Large Rivers: Towards Sustainable Rivers Management under Altered Flows

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    Kuriqi, Alban; Rosário Fernandes, M.; Santos, Artur; Ferreira, M. Teresa

    2017-04-01

    Hydromorphological patterns changes in large rivers, result from a long history of human interventions. In this study, we evaluate the causes and effects of hydromorphological alterations in the Iberian Minho River using a planform change analysis. We performed a temporal comparison using historical maps (nineteen century) and contemporaneous maps. The studied river was divided in 2.5 km long river stretches in a total of 25 sampling units. The historical maps were initially georeferenced for the WGS84 coordinate system. We used Geographic Information System (GIS) to extract the hydromorphological features and to store and organised the spatial data. The hydromorphological features (sinuosity index, braiding intensity, river corridor and active channel width, lotic and lentic habitats) were mapped by visual interpretation of the historical and the contemporaneous maps on a scale 1:2500 by applying the same methodology. Also, we analysed certain Indicators of Hydrological Alteration (IHA) based on pre- and post-dam daily streamflow data obtained from the Spanish Water Information System (SIA). The results revealed a significant reduction in the active channel width and all sinuosity indexes representing an overall degradation of river conditions. We also noticed a drastic diminution in the number and total area of lentic habitats causing fish habitat shifts. Changes were less evident in upstream sampling units due to diverse Land Use/Land Cover (LULC) changes combine with some geological constraints. These responses were consistent with reductions in mean annual discharge, flood disturbance decrease and minimum flow increase during the summer season. This work allows to understand the evolutionary trajectory of large fluvial system over more than 100 years and to implement concrete measures for sustainable river management. Keywords: historical maps, large rivers, flow alteration, sinuosity index, lotic and lentic habitats, regulated rivers, river restoration.

  13. Big River Benthos: Linking Year Round Biological Response to Altered Hydrological Regimes

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    2017-04-02

    Big River Benthos: Linking Year- Round Biological Response to Altered Hydrological Regimes by Audrey B. Harrison , Clifford A. Ochs...Audrey B. Harrison , audrey.b.harrison@erdc.dren.mil or Jack Killgore, jack.killgore@usace.army.mil. This technical note should be cited as follows... Harrison , A. B., C. A. Ochs, W. T. Slack, and K. J. Killgore. 2017. "Big river benthos: linking year-round biological response to altered

  14. Alterations of River Flow Caused By Dams. The Ebro River (ne Spain)

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    Batalla, R. J.; Kondolf, G. M.

    The Ebro River drains 85,530 km2 of the Cantabrian Range, Pyrenees, and Iberian Massif in northeastern Spain, with a mean annual runoff of 13,400 106 m3 at Tortosa, where it debouches into the Mediterranean Sea, about 180 km south of Barcelona. One hundred eighty-seven reservoirs (two-thirds built between 1950 and 1975) built for hydroelectric production, irrigation, cooling water, and industrial and domestic uses, have a total capacity equivalent to 57% of the Ebro River's mean annual runoff. Gauging records are available from the Confederación Hidrográfica del Ebro (CHE), a government agency established in 1926 to manage the water resources in the Ebro River basin. We analyzed 38 gauging records from 22 rivers that, by virtue of their location within the drainage network and period of record, would reflect hydrological changes from reservoir construction and operation. From pre- and post-dam records, we analyzed changes in flood peaks, mean annual runoff, mean daily flows, and mean monthly flows for four distinct climatic zones. Most rivers showed reduction in flood magnitude, with average reduction of over 30% for Q2 and Q10. Greater reductions were associated with higher values of the Impounded Runoff index (IR, calculated as reservoir capacity divided by mean annual runoff). Despite similar values of IR, floods in the low-rainfall Mediterranean tributaries in the southeast part of the basin were more affected by reservoirs than those in the high-rainfall humid Atlantic tributaries in the western part of the basin, with a given percentage of regulation producing twice the flood reduction as in the humid Atlantic zone. Annual runoff did not show strong trends, but the variability of mean daily flows was reduced in most cases due to storing of winter floods and increased baseflows in summer for irrigation. Monthly flows ranged from virtually no change post-dam to complete inversion in seasonal pattern, the latter due to releases for irrigation in the summer

  15. Impact of climate change on freshwater ecosystems: a global-scale analysis of ecologically relevant river flow alterations

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    P. Döll

    2010-05-01

    Full Text Available River flow regimes, including long-term average flows, seasonality, low flows, high flows and other types of flow variability, play an important role for freshwater ecosystems. Thus, climate change affects freshwater ecosystems not only by increased temperatures but also by altered river flow regimes. However, with one exception, transferable quantitative relations between flow alterations and ecological responses have not yet been derived. While discharge decreases are generally considered to be detrimental for ecosystems, the effect of future discharge increases is unclear. As a first step towards a global-scale analysis of climate change impacts on freshwater ecosystems, we quantified the impact of climate change on five ecologically relevant river flow indicators, using the global water model WaterGAP 2.1g to simulate monthly time series of river discharge with a spatial resolution of 0.5 degrees. Four climate change scenarios based on two global climate models and two greenhouse gas emissions scenarios were evaluated.

    We compared the impact of climate change by the 2050s to the impact of water withdrawals and dams on natural flow regimes that had occurred by 2002. Climate change was computed to alter seasonal flow regimes significantly (i.e. by more than 10% on 90% of the global land area (excluding Greenland and Antarctica, as compared to only one quarter of the land area that had suffered from significant seasonal flow regime alterations due to dams and water withdrawals. Due to climate change, the timing of the maximum mean monthly river discharge will be shifted by at least one month on one third on the global land area, more often towards earlier months (mainly due to earlier snowmelt. Dams and withdrawals had caused comparable shifts on less than 5% of the land area only. Long-term average annual river discharge is predicted to significantly increase on one half of the land area, and to significantly decrease on one quarter

  16. Impact of climate change on freshwater ecosystems: a global-scale analysis of ecologically relevant river flow alterations

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    P. Döll

    2010-02-01

    Full Text Available River flow regimes, including long-term average flows, seasonality, low flows, high flows and other types of flow variability, play an important role for freshwater ecosystems. Thus, climate change affects freshwater ecosystems not only by increased temperatures but also by altered river flow regimes. However, with one exception, transferable quantitative relations between flow alterations and ecosystem responses have not yet been derived. While discharge decreases are generally considered to be detrimental for ecosystems, the effect of future discharge increases is unclear. As a first step towards a global-scale analysis of climate change impacts on freshwater ecosystems, we quantified the impact of climate change on five ecologically relevant river flow indicators, using the global water model WaterGAP 2.1g to simulate monthly time series of river discharge with a spatial resolution of 0.5 degrees. Four climate change scenarios based on two global climate models and two greenhouse gas emissions scenarios were evaluated.

    We compared the impact of climate change by the 2050s to the impact of water withdrawals and dams on natural flow regimes that had occurred by 2002. Climate change was computed to alter seasonal flow regimes significantly (i.e. by more than 10% on 90% of the global land area (excluding Greenland and Antarctica, as compared to only one quarter of the land area that had suffered from significant seasonal flow regime alterations due to dams and water withdrawals. Due to climate change, the timing of the maximum mean monthly river discharge will be shifted by at least one month on one third on the global land area, more often towards earlier months (mainly due to earlier snowmelt. Dams and withdrawals had caused comparable shifts on less than 5% of the land area only. Long-term average annual river discharge is predicted to significantly increase on one half of the land area, and to significantly decrease on one quarter

  17. Using Pressure and Alteration Indicators to Assess River Morphological Quality: Case Study of the Prahova River (Romania

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    Gabriela Ioana-Toroimac

    2015-06-01

    Full Text Available River morphological quality assessment, derived from quantification of human pressures as well as river channel alteration, is a demand of the Water Framework Directive (WFD in terms of integrating hydromorphological elements in defining ecological status. Our study’s aim is to contribute to the hydromorphological evaluation by proposing indicators and separating classes, based on a revisited Morphological Quality Index (rMQI protocol. The rMQI is based on 12 indicators of human pressures, 10 indicators of channel form adjustments, and 11 indicators of functionality. The rMQI scoring system allows for the quantification of changes when compared to reference conditions, be they undisturbed or nearly undisturbed by human interventions, with absent channel adjustments and a functioning natural river style. We used the lower, meandering sector of the Prahova River to demonstrate our assessment methodology. The Lower Prahova River suffers from a minor local intervention and a diminishing intensity of fluvial processes specific to a meandering style. Meanders geometry was affected by significant changes that included a decrease in the radius of curvature, width and width–to–mean–depth ratio. We concluded that the Lower Prahova River has a good morphological quality, which is rated as second class on a scale of five levels, from natural to severely modified. We recommend an improvement in the hydromorphological evaluation protocol in Romania by additional indicators for morphological alterations specific to each channel pattern.

  18. Carbon dynamics of river corridors and the effects of human alterations

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    Wohl, Ellen; Hall, Robert O.; Lininger, Katherine B; Sutfin, Nicholas A.; Walters, David

    2017-01-01

    Research in stream metabolism, gas exchange, and sediment dynamics indicates that rivers are an active component of the global carbon cycle and that river form and process can influence partitioning of terrestrially derived carbon among the atmosphere, geosphere, and ocean. Here we develop a conceptual model of carbon dynamics (inputs, outputs, and storage of organic carbon) within a river corridor, which includes the active channel and the riparian zone. The exchange of carbon from the channel to the riparian zone represents potential for storage of transported carbon not included in the “active pipe” model of organic carbon (OC) dynamics in freshwater systems. The active pipe model recognizes that river processes influence carbon dynamics, but focuses on CO2 emissions from the channel and eventual delivery to the ocean. We also review how human activities directly and indirectly alter carbon dynamics within river corridors. We propose that dams create the most significant alteration of carbon dynamics within a channel, but that alteration of riparian zones, including the reduction of lateral connectivity between the channel and riparian zone, constitutes the most substantial change of carbon dynamics in river corridors. We argue that the morphology and processes of a river corridor regulate the ability to store, transform, and transport OC, and that people are pervasive modifiers of river morphology and processes. The net effect of most human activities, with the notable exception of reservoir construction, appears to be that of reducing the ability of river corridors to store OC within biota and sediment, which effectively converts river corridors to OC sources rather than OC sinks. We conclude by summarizing knowledge gaps in OC dynamics and the implications of our findings for managing OC dynamics within river corridors.

  19. ALTERED DEVELOPMENT AND REPRODUCTION IN MOSQUITOFISH EXPOSED TO PULP AND PAPER MILL EFFLUENT IN THE FENHOLLOW RIVER, FLORIDA USA

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    Female mosquitofish exposed to pulp and paper mill effluent (PME) in the Fenholloway River, Florida, USA have masculinized secondary sex characteristics and altered aromatase enzyme activity. We and others have shown that the Fenholloway River PME contains androgenic and progesto...

  20. Impacts of Climate Change on Riverine Ecosystems: Alterations of Ecologically Relevant Flow Dynamics in the Danube River and Its Major Tributaries

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    Judith C. Stagl

    2016-12-01

    Full Text Available River flow dynamics play an important role for aquatic and riparian ecosystems. Climate change is projected to significantly alter river flow regimes in Europe and worldwide. In this study, we evaluate future river flow alterations in the entire Danube River basin by means of ecologically relevant river flow indicators under different climate warming scenarios (Representative Concentration Pathway (RCP 2.6, RCP 4.5, and RCP 8.5. The process-based watershed model SWIM was applied for 1124 sub-catchments to simulate daily time series of river discharge for the Danube River and its tributaries under future scenario conditions. The derived hydrological data series were then statistically analyzed using eight eco-hydrological indicators to distinguish intra-year variations in the streamflow regime. The results are used to: (a analyze the possible impacts of climate change on the ecologically relevant flow regime components; and (b identify regions at the highest risk of climate change-driven flow alterations. Our results indicate that climate change will distinctively alter the recent ecological flow regime of the Danube River and, in particular, the tributaries of the Middle and Lower Danube basin. While for the RCP 2.6 scenario the projected flow alterations might still be considered moderate for many rivers, the impacts might strongly accelerate if global mean temperatures rise more than 2 °C compared to pre-industrial times. Under RCP 4.5 and RCP 8.5 warming scenarios, the recent ecological flow regime might be highly altered, posing a serious threat to river and floodplain ecosystems.

  1. Predicting the likelihood of altered streamflows at ungauged rivers across the conterminous United States

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    Eng, Kenny; Carlisle, Daren M.; Wolock, David M.; Falcone, James A.

    2013-01-01

    An approach is presented in this study to aid water-resource managers in characterizing streamflow alteration at ungauged rivers. Such approaches can be used to take advantage of the substantial amounts of biological data collected at ungauged rivers to evaluate the potential ecological consequences of altered streamflows. National-scale random forest statistical models are developed to predict the likelihood that ungauged rivers have altered streamflows (relative to expected natural condition) for five hydrologic metrics (HMs) representing different aspects of the streamflow regime. The models use human disturbance variables, such as number of dams and road density, to predict the likelihood of streamflow alteration. For each HM, separate models are derived to predict the likelihood that the observed metric is greater than (‘inflated’) or less than (‘diminished’) natural conditions. The utility of these models is demonstrated by applying them to all river segments in the South Platte River in Colorado, USA, and for all 10-digit hydrologic units in the conterminous United States. In general, the models successfully predicted the likelihood of alteration to the five HMs at the national scale as well as in the South Platte River basin. However, the models predicting the likelihood of diminished HMs consistently outperformed models predicting inflated HMs, possibly because of fewer sites across the conterminous United States where HMs are inflated. The results of these analyses suggest that the primary predictors of altered streamflow regimes across the Nation are (i) the residence time of annual runoff held in storage in reservoirs, (ii) the degree of urbanization measured by road density and (iii) the extent of agricultural land cover in the river basin.

  2. Modeling Typhoon‐Induced Alterations on River Sediment Transport and Turbidity Based on Dynamic Landslide Inventories: Gaoping River Basin, Taiwan

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    Chih‐Hua Chang

    2015-12-01

    Full Text Available This study examines the impacts of storm‐triggered landslides on downstream sediment and turbidity responses in the Gaoping River Basin, Taiwan using the Soil and Water Assessment Tool (SWAT. Attention is given to analyzing the increased and altered baseline of suspended sediment load and turbidity after the disturbances caused by the rainfall and landslides associated with Typhoon Morakot in 2009. SWAT parameters were calibrated by the observed hydrometric data from 1999 to 2003 using the log‐scale root‐mean‐square error (log‐RMSE and Nash‐Sutcliffe Model Efficiency. Both parameter sets were applied for the simulation of suspended sediment yield and turbidity with annual updated landslide inventories for the period 2004–2012. The landslide updating mirrors the physical land‐cover changes and has slightly improved the model performance, yet landslides alone cannot explain the difference between Morakot‐induced and SWAT‐simulated sediment discharge. The set of parameters calibrated by log‐RMSE can better approximate the increased baseline and typhoon induced alterations. The results show alterations in sediment erosion and transport: (1 drastically increased the turbidity baseline and occurrence of high‐turbidity; (2 altered coefficient and exponent values of the sediment rating curve; and (3 altered relationship between rainfall and induced turbidity during major rainfall events. The research in this study provides an improved modeling approach to typhoon‐induced alterations on river sediment loads and turbidity.

  3. Global-scale analysis of river flow alterations due to water withdrawals and reservoirs

    Science.gov (United States)

    Döll, P.; Fiedler, K.; Zhang, J.

    2009-12-01

    Global-scale information on natural river flows and anthropogenic river flow alterations is required to identify areas where aqueous ecosystems are expected to be strongly degraded. Such information can support the identification of environmental flow guidelines and a sustainable water management that balances the water demands of humans and ecosystems. This study presents the first global assessment of the anthropogenic alteration of river flow regimes, in particular of flow variability, by water withdrawals and dams/reservoirs. Six ecologically relevant flow indicators were quantified using an improved version of the global water model WaterGAP. WaterGAP simulated, with a spatial resolution of 0.5 degree, river discharge as affected by human water withdrawals and dams around the year 2000, as well as naturalized discharge without this type of human interference. Compared to naturalized conditions, long-term average global discharge into oceans and internal sinks has decreased by 2.7% due to water withdrawals, and by 0.8% due to dams. Mainly due to irrigation, long-term average river discharge and statistical low flow Q90 (monthly river discharge that is exceeded in 9 out of 10 months) have decreased by more than 10% on one sixth and one quarter of the global land area (excluding Antarctica and Greenland), respectively. Q90 has increased significantly on only 5% of the land area, downstream of reservoirs. Due to both water withdrawals and reservoirs, seasonal flow amplitude has decreased significantly on one sixth of the land area, while interannual variability has increased on one quarter of the land area mainly due to irrigation. It has decreased on only 8% of the land area, in areas downstream of reservoirs where consumptive water use is low. The impact of reservoirs is likely underestimated by our study as small reservoirs are not taken into account. Areas most affected by anthropogenic river flow alterations are the Western and Central USA, Mexico, the

  4. Global-scale analysis of river flow alterations due to water withdrawals and reservoirs

    Directory of Open Access Journals (Sweden)

    P. Döll

    2009-07-01

    Full Text Available Global-scale information on natural river flows and anthropogenic river flow alterations is required to identify areas where aqueous ecosystems are expected to be strongly degraded. Such information can support the identification of environmental flow guidelines and a sustainable water management that balances the water demands of humans and ecosystems. This study presents the first global assessment of the anthropogenic alteration of river flow regimes by water withdrawals and dams, focusing in particular on the change of flow variability. Six ecologically relevant flow indicators were quantified using an improved version of the global water model WaterGAP. WaterGAP simulated, with a spatial resolution of 0.5 degree, river discharge as affected by human water withdrawals and dams, as well as naturalized discharge without this type of human interference. Mainly due to irrigation, long-term average river discharge and statistical low flow Q90 (monthly river discharge that is exceeded in 9 out of 10 months have decreased by more than 10% on one sixth and one quarter of the global land area (excluding Antarctica and Greenland, respectively. Q90 has increased significantly on only 5% of the land area, downstream of reservoirs. Due to both water withdrawals and dams, seasonal flow amplitude has decreased significantly on one sixth of the land area, while interannual variability has increased on one quarter of the land area mainly due to irrigation. It has decreased on only 8% of the land area, in areas with little consumptive water use that are downstream of dams. Areas most affected by anthropogenic river flow alterations are the western and central USA, Mexico, the western coast of South America, the Mediterranean rim, Southern Africa, the semi-arid and arid countries of the Near East and Western Asia, Pakistan and India, Northern China and the Australian Murray-Darling Basin, as well as some Arctic rivers. Due

  5. Global-scale analysis of river flow alterations due to water withdrawals and reservoirs

    Directory of Open Access Journals (Sweden)

    P. Döll

    2009-12-01

    Full Text Available Global-scale information on natural river flows and anthropogenic river flow alterations is required to identify areas where aqueous ecosystems are expected to be strongly degraded. Such information can support the identification of environmental flow guidelines and a sustainable water management that balances the water demands of humans and ecosystems. This study presents the first global assessment of the anthropogenic alteration of river flow regimes, in particular of flow variability, by water withdrawals and dams/reservoirs. Six ecologically relevant flow indicators were quantified using an improved version of the global water model WaterGAP. WaterGAP simulated, with a spatial resolution of 0.5 degree, river discharge as affected by human water withdrawals and dams around the year 2000, as well as naturalized discharge without this type of human interference. Compared to naturalized conditions, long-term average global discharge into oceans and internal sinks has decreased by 2.7% due to water withdrawals, and by 0.8% due to dams. Mainly due to irrigation, long-term average river discharge and statistical low flow Q90 (monthly river discharge that is exceeded in 9 out of 10 months have decreased by more than 10% on one sixth and one quarter of the global land area (excluding Antarctica and Greenland, respectively. Q90 has increased significantly on only 5% of the land area, downstream of reservoirs. Due to both water withdrawals and reservoirs, seasonal flow amplitude has decreased significantly on one sixth of the land area, while interannual variability has increased on one quarter of the land area mainly due to irrigation. It has decreased on only 8% of the land area, in areas downstream of reservoirs where consumptive water use is low. The impact of reservoirs is likely underestimated by our study as small reservoirs are not taken into account. Areas most affected by anthropogenic river flow

  6. Opportunities for Process-Based Management of Rivers Below Water Diversions: Exploring and Exploiting Coupled Alteration of Flow and Sediment Regimes

    Science.gov (United States)

    Kibler, K. M.; Alipour, M.

    2015-12-01

    In mountainous, rural catchments throughout the world, small to medium-sized rivers are being developed for diversion hydropower. Previous study indicates that in the absence of careful management, consequences to rivers downstream of diversion hydropower dams can be severe. However, patterns of hydrologic alteration below diversion hydropower dams have not been well described. In addition, diversion hydropower projects typically require some form of sediment management to maintain relatively small impoundments at diversion sites. Investigation of principal coupled processes, notably flow and sediment dynamics, in the context of alteration by diversion schemes may elucidate potential for working with natural processes to mitigate consequences to rivers. This analysis characterizes hydrologic alteration by diversion hydropower dams, investigating flows below 31 diversion dams. Diversion for hydropower production generated distinctive signatures of hydrologic alteration in sample rivers. For instance, flow signals were characterized by reduced low to moderate flows and steep transitions between low and high stages. In a number of rivers, small floods were reduced in number and size. However, effect to magnitude and timing of large floods was minimal across all rivers investigated. Sediments were stored in impoundments and released in pulses annually through lower sediment gates. The hydrologic signatures observed were strongly linked to diversion designs and may be transferable to other rivers developed with similar infrastructure. Understanding characteristic flow alterations due to diversion hydropower, in combination with cycles of sediment storage and release, may inform management practices for simultaneously maintaining reservoirs and downstream channels. In particular, investigating altered flood magnitudes and frequencies in the context of geomorphic work may indicate potential for maintaining natural hydrogeomorphic processes. Detailed study of sediment

  7. Evidence of altered fertility in female roach (Rutilus rutilus) from the River Seine (France).

    Science.gov (United States)

    Gerbron, M; Geraudie, P; Fernandes, D; Rotchell, J M; Porte, C; Minier, C

    2014-08-01

    A large variety of anthropogenic chemicals present in the aquatic environment have been shown to be able to alter the endocrine system of exposed organisms, potentially impacting their reproductive function. The aim of this study was to assess the effects of environmental pollution on the reproductive system of wild female roach (Rutilus rutilus) from the Seine River (Normandy, France). A suite of biomarkers of endocrine disruption including gonado-somatic index, plasmatic vitellogenin, gonadal aromatase activity and histological parameters (oocyte diameter and gonad maturation) were studied. Female fish from the polluted sites showed a number of reproductive alterations, including inhibited gonad maturation, reduced oocyte growth, reduced levels of plasmatic vitellogenin and 3-fold lower gonadal aromatase activity than females collected in the reference site. Overall, these results highlight the presence of endocrine disruption in female roach from the Seine River. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Geomorphic Framework to assess changes to aquatic habitat due to flow regulation and channel and floodplain alteration, Cedar River, Washington

    Science.gov (United States)

    Gendaszek, Andrew S.; Magirl, Christopher S.; Czuba, Christiana R.; Konrad, Christopher P.; Little, Rand

    2010-01-01

    Flow regulation, bank armoring, and floodplain alteration since the early 20th century have contributed to significant changes in the hydrologic regime and geomorphic processes of the Cedar River in Washington State. The Cedar River originates in the Cascade Range, provides drinking water to the Seattle metropolitan area, and supports several populations of anadromous salmonids. Flow regulation currently has limited influence on the magnitude, duration, and timing of high-flow events, which affect the incubation of salmonids as well as the production and maintenance of their habitat. Unlike structural changes to the channel and floodplain, flow regulation may be modified in the short-term to improve the viability of salmon populations. An understanding of the effects of flow regulation on those populations must be discerned over a range of scales from individual floods that affect the size of individual year classes to decadal high flow regime that influences the amount and quality of channel and off-channel habitat available for spawning and rearing. We present estimates of reach-scale sediment budgets and changes to channel morphology derived from historical orthoimagery, specific gage analyses at four long-term streamflow-gaging stations to quantify trends in aggradation, and hydrologic statistics of the magnitude and duration of peak streamflows. These data suggest a gradient of channel types from unconfined, sediment-rich segments to confined, sediment-poor segments that are likely to have distinct responses to high flows. Particle-size distribution data and longitudinal water surface and streambed profiles for the 56 km downstream of Chester Morse Lake measured in 2010 show the spatial extent of preferred salmonid habitat along the Cedar River. These historical and current data constitute a geomorphic framework to help assess different river management scenarios for salmonid habitat and population viability. PDF version of a presentation on changes to aquatic

  9. Floodplain geomorphic processes and environmental impacts of human alteration along coastal plain rivers, USA

    Science.gov (United States)

    Hupp, C.R.; Pierce, A.R.; Noe, G.B.

    2009-01-01

    Human alterations along stream channels and within catchments have affected fluvial geomorphic processes worldwide. Typically these alterations reduce the ecosystem services that functioning floodplains provide; in this paper we are concerned with the sediment and associated material trapping service. Similarly, these alterations may negatively impact the natural ecology of floodplains through reductions in suitable habitats, biodiversity, and nutrient cycling. Dams, stream channelization, and levee/canal construction are common human alterations along Coastal Plain fluvial systems. We use three case studies to illustrate these alterations and their impacts on floodplain geomorphic and ecological processes. They include: 1) dams along the lower Roanoke River, North Carolina, 2) stream channelization in west Tennessee, and 3) multiple impacts including canal and artificial levee construction in the central Atchafalaya Basin, Louisiana. Human alterations typically shift affected streams away from natural dynamic equilibrium where net sediment deposition is, approximately, in balance with net erosion. Identification and understanding of critical fluvial parameters (e.g., stream gradient, grain-size, and hydrography) and spatial and temporal sediment deposition/erosion process trajectories should facilitate management efforts to retain and/or regain important ecosystem services. ?? 2009, The Society of Wetland Scientists.

  10. When hillslope-derived blocks alter river evolution: A sensitivity analysis

    Science.gov (United States)

    Shobe, C. M.; Tucker, G. E.

    2016-12-01

    Many studies rely on simple models of river channel evolution for predicting channel response to perturbations and landscape evolution outcomes, as well as extracting climatic and tectonic signals from river longitudinal profiles. However, recent work has shown that large, hillslope-derived blocks delivered to rivers in response to rapid incision may noticeably alter the form and evolution of river profiles from the behavior predicted by the most common models. We use a 1-D model of fluvial erosion and block dynamics to investigate the conditions under which this channel-hillslope feedback influences river erosion and landscape evolution. In the model, block delivery by hillslopes occurs in response to channel bed lowering, and blocks inhibit further erosion by covering the bed and reducing effective bed shear stress. Using formal sensitivity analysis techniques including Morris one-at-a-time screening and variance-based decomposition, we seek to explore the full model parameter space and determine which landscape parameters set the influence of blocks on river incision. We use eight response functions to evaluate model behavior, including the mean, standard deviation, and range of the distributions of elevation and slope in the model domain. Preliminary results indicate that baselevel lowering rate, bed erodibility, initial block size, and a parameter governing the connection between river erosion and volume flux of blocks to the channel control whether or not channel evolution will be influenced by hillslope-derived blocks. Surprisingly, block erodibility and the delay timescale separating channel incision from hillslope response have relatively little influence on model outcomes. Results also show that parameter sensitivity is strongly dependent on the chosen response function, highlighting the need for multiple model metrics. Identification of the most important model parameters allows confident identification of landscapes strongly influenced by block delivery

  11. Inventory of Long-Term Braiding Activity at a Regional Scale as a Tool for Detecting Alterations to a Rivers' Hydromorphological State: A Case Study for Romania's South-Eastern Subcarpathians

    Science.gov (United States)

    Ioana-Toroimac, Gabriela

    2016-07-01

    The inventory of long-term braiding activity is a useful tool for detecting alterations in a rivers' hydromorphological state and for a river's management in the context of the Water Framework Directive on integrated river basin management for Europe. Our study focuses on braided sectors of rivers in South-Eastern Subcarpathians (Romania). The inventory evaluates types of alterations based on the spatial analysis of fluvial morphology indicators (i.e., length of the river sector forming a braided pattern; width of the braided active channel), and vegetation cover (i.e., length of banks covered by forest and shrubs; area of in-stream patches of shrubs) accumulated over the last century. Furthermore, we performed a regional scale hierarchical cluster analysis to estimate the degree of alteration when compared to an historical baseline. In South-Eastern Subcarpathians, the studied rivers experienced a decrease of braiding activity revealed by the shortening and narrowing of their braided sectors, expansion of riparian forests, and the diminishment of vegetated islands' areas. We separated three types of river clusters, corresponding to low (cluster 1), moderate (cluster 2), and high (cluster 3) degree of alteration. Moreover, the clusters demonstrate the evolutionary path of the braided pattern alterations until the functioning of another channel pattern. The inventory is relevant for differing types and levels of alterations. Additionally, this tool may serve as a first step toward the restoration of altered sectors by identifying rivers in cluster 1 as potential candidates of present-day reference sites for altered rivers with similar natural conditions as in cluster 3.

  12. THE IMPACT OF THE KOWALSKIE RESERVOIR ON THE HYDROLOGICAL REGIME ALTERATION OF THE GŁÓWNA RIVER

    Directory of Open Access Journals (Sweden)

    Mariusz Sojka

    2016-09-01

    Full Text Available This paper present an assessment of the impact of the Kowalskie reservoir on the hydrological regime alteration of the Główna river. The Główna river is a right tributary of the Warta river. The Kowalskie reservoir was built in 1984. The area of the reservoir in the normal condition is 203 ha and total capacity is 6.58×106 m3. The impact of the Kowalskie reservoir on the hydrological regime was made on the basis of RVA method. The assessment was based on the analysis of flow series from the period of 1971–2012. The calculations were done by means of IHA software version 7.1.0.10 developed by The Nature Conservancy (2009. This study indicate that the Kowalskie reservoir has an impact on two group of flow parameters that characterized frequency and duration of high and low pulses as well as rate and frequency of water condition change. The others flow parameters characterized magnitude and duration of annual extreme water conditions are less affected by the Kowalskie reservoir. This study show that Kowalskie reservoir play important role in flood protection. Additionally the reservoir operation has an positive effect on minimum flows characteristic and base flow index.

  13. Natural streamflow simulation for two largest river basins in Poland: a baseline for identification of flow alterations

    Science.gov (United States)

    Piniewski, Mikołaj

    2016-05-01

    The objective of this study was to apply a previously developed large-scale and high-resolution SWAT model of the Vistula and the Odra basins, calibrated with the focus of natural flow simulation, in order to assess the impact of three different dam reservoirs on streamflow using the Indicators of Hydrologic Alteration (IHA). A tailored spatial calibration approach was designed, in which calibration was focused on a large set of relatively small non-nested sub-catchments with semi-natural flow regime. These were classified into calibration clusters based on the flow statistics similarity. After performing calibration and validation that gave overall positive results, the calibrated parameter values were transferred to the remaining part of the basins using an approach based on hydrological similarity of donor and target catchments. The calibrated model was applied in three case studies with the purpose of assessing the effect of dam reservoirs (Włocławek, Siemianówka and Czorsztyn Reservoirs) on streamflow alteration. Both the assessment based on gauged streamflow (Before-After design) and the one based on simulated natural streamflow showed large alterations in selected flow statistics related to magnitude, duration, high and low flow pulses and rate of change. Some benefits of using a large-scale and high-resolution hydrological model for the assessment of streamflow alteration include: (1) providing an alternative or complementary approach to the classical Before-After designs, (2) isolating the climate variability effect from the dam (or any other source of alteration) effect, (3) providing a practical tool that can be applied at a range of spatial scales over large area such as a country, in a uniform way. Thus, presented approach can be applied for designing more natural flow regimes, which is crucial for river and floodplain ecosystem restoration in the context of the European Union's policy on environmental flows.

  14. Sensitivity and tolerance of Riparian arthropod communities to altered water resources along a drying river.

    Directory of Open Access Journals (Sweden)

    Kevin E McCluney

    Full Text Available BACKGROUND: Rivers around the world are drying with increasing frequency, but little is known about effects on terrestrial animal communities. Previous research along the San Pedro River in southeastern AZ, USA, suggests that changes in the availability of water resources associated with river drying lead to changes in predator abundance, community composition, diversity, and abundance of particular taxa of arthropods, but these observations have not yet been tested manipulatively. METHODS AND RESULTS: In this study, we constructed artificial pools in the stream bed adjacent to a drying section of the San Pedro River and maintained them as the river dried. We compared pitfall trapped arthropods near artificial pools to adjacent control sites where surface waters temporarily dried. Assemblage composition changed differentially at multiple taxonomic levels, resulting in different assemblages at pools than at control sites, with multiple taxa and richness of carabid beetle genera increasing at pools but not at controls that dried. On the other hand, predator biomass, particularly wolf spiders, and diversity of orders and families were consistently higher at control sites that dried. These results suggest an important role for colonization dynamics of pools, as well as the ability of certain taxa, particularly burrowing wolf spiders, to withstand periods of temporary drying. CONCLUSIONS: Overall, we found some agreement between this manipulative study of water resources and a previous analysis of river drying that showed shifts in composition, changes in diversity, and declines in abundance of certain taxa (e.g. carabid beetles. However, colonization dynamics of pools, as well as compensatory strategies of predatory wolf spiders seem to have led to patterns that do not match previous research, with control sites maintaining high diversity, despite drying. Tolerance of river drying by some species may allow persistence of substantial diversity in the

  15. High-fat diet alters gut microbiota physiology in mice

    National Research Council Canada - National Science Library

    Daniel, Hannelore; Gholami, Amin Moghaddas; Berry, David; Desmarchelier, Charles; Hahne, Hannes; Loh, Gunnar; Mondot, Stanislas; Lepage, Patricia; Rothballer, Michael; Walker, Alesia; Böhm, Christoph; Wenning, Mareike; Wagner, Michael; Blaut, Michael; Schmitt-Kopplin, Philippe; Kuster, Bernhard; Haller, Dirk; Clavel, Thomas

    2014-01-01

    ...) metaproteome and metabolome via high-resolution mass spectrometry. High-fat diet caused shifts in the diversity of dominant gut bacteria and altered the proportion of Ruminococcaceae (decrease) and Rikenellaceae (increase...

  16. Diagenetic alterations of amino acids and organic matter in the upper Pearl River Estuary surface sediments

    Directory of Open Access Journals (Sweden)

    J. Zhang

    2011-03-01

    Full Text Available The objective of this study was to investigate the sources, diagenetic alterations of, and bacterial contributions to sediment organic matter (OM in the upper Pearl River Estuary. Sediment analyses were conducted for three size fractions of OM, including coarse particulate OM (CPOM, fine particulate OM (FPOM, and ultrafiltered dissolved OM (UDOM. Results showed that the highest and lowest carbon (C: nitrogen (N ratios were in CPOM and UDOM, respectively, indicating CPOM was relatively enriched in organic C, whereas FPOM was enriched in N-containing molecules. Distributions of amino acids and their D-isomers among the sediment fractions indicated that the percentage of total N represented by total hydrolysable amino acids, C- and N-normalized yields of total D-amino acids, and C- and N-normalized yields of D-alanine, D-glutamic acid, D-serine could be used as diagenetic indicators of sediment OM. Correlations between the N yields in total D-amino acids and total hydrolysable amino acids, and total N yields suggested that the bacterial N in general reflected the bulk N changes in CPOM, FPOM, and UDOM. Our results demonstrate the crucial role of bacteria as a N source in the terrestrial (soil and vascular plant debris OM transported by the river.

  17. Effects of flow regimes altered by dams on survival, population declines, and range-wide losses of California river-breeding frogs.

    Science.gov (United States)

    Kupferberg, Sarah J; Palen, Wendy J; Lind, Amy J; Bobzien, Steve; Catenazzi, Alessandro; Drennan, Joe; Power, Mary E

    2012-06-01

    Widespread alteration of natural hydrologic patterns by large dams combined with peak demands for power and water delivery during summer months have resulted in frequent aseasonal flow pulses in rivers of western North America. Native species in these ecosystems have evolved with predictable annual flood-drought cycles; thus, their likelihood of persistence may decrease in response to disruption of the seasonal synchrony between stable low-flow conditions and reproduction. We evaluated whether altered flow regimes affected 2 native frogs in California and Oregon (U.S.A.) at 4 spatial and temporal extents. We examined changes in species distribution over approximately 50 years, current population density in 11 regulated and 16 unregulated rivers, temporal trends in abundance among populations occupying rivers with different hydrologic histories, and within-year patterns of survival relative to seasonal hydrology. The foothill yellow-legged frog (Rana boylii), which breeds only in flowing water, is more likely to be absent downstream of large dams than in free-flowing rivers, and breeding populations are on average 5 times smaller in regulated rivers than in unregulated rivers. Time series data (range = 8 - 19 years) from 5 populations of yellow-legged frogs and 2 populations of California red-legged frogs (R. draytonii) across a gradient of natural to highly artificial timing and magnitude of flooding indicate that variability of flows in spring and summer is strongly correlated with high mortality of early life stages and subsequent decreases in densities of adult females. Flow management that better mimics natural flow timing is likely to promote persistence of these species and others with similar phenology.

  18. Endocrine modulation, inhibition of ovarian development and hepatic alterations in rainbow trout exposed to polluted river water

    Energy Technology Data Exchange (ETDEWEB)

    Vigano, Luigi, E-mail: vigano@irsa.cnr.i [Water Research Institute, National Council of Research, Brugherio, Milan (Italy); Benfenati, Emilio [Mario Negri Institute, Laboratory of Environmental Chemistry and Toxicology, Milan (Italy); Bottero, Sergio; Cevasco, Alessandra; Monteverde, Martino; Mandich, Alberta [Department of Environmental, Experimental and Applied Biology, University of Genoa, Genoa (Italy)

    2010-12-15

    Under laboratory conditions, female rainbow trout were exposed to graded concentrations of water from the River Lambro, a polluted tributary of the River Po, and to the effluent of a large wastewater treatment plant which flows into the River Lambro. In field exposures, trout were held in cages in the River Po upstream and downstream from the confluence of the River Lambro. After 10-day (laboratory) and 30-day (laboratory and field) exposures, trout were examined for several chemical, biochemical and histological endpoints. The results indicated that exposure to complex mixtures of chemicals, including estrogen receptor agonists, aryl-hydrocarbon receptor agonists, and probably antiandrogens, had occurred. Exposure altered the plasma levels of 17{beta}-estradiol and testosterone, and some treatments also enhanced the activity of hepatic ethoxyresorufin O-deethylase. Gonadal histology showed varying levels of degenerative processes characterised by oocyte atresia, haemorrhages, melano-macrophage centres (MMCs), and oogonia proliferation. Liver histology showed less severe effects. - This study examined the progression of hormonal and gonadal alterations in female trout exposed to river water from an area known to affect resident fish species.

  19. Integrated approach to assessing streamflow and precipitation alterations under environmental change: Application in the Niger River Basin

    Directory of Open Access Journals (Sweden)

    Dagbegnon Clement Sohoulande Djebou

    2015-09-01

    New hydrological insights for the region: Over the period 1961–2012, I conduct a change point analysis of the streamflow and report two sub-periods 1961–1982 and 1983–2012. A comparison of precipitation and streamflow during these two time-slices shows meaningful changes. I describe a Kernel density analysis of streamflow and yield a probabilistic estimate of discharge anomalies along the river. Later, I evaluate seasonal trends of precipitation and streamflow. The analyses bring out critical alterations in time and space. However, these alterations seem to foreshadow critical environmental degradations occurring across the watershed. I consider LAI series derived from MODIS images, then I examine and discuss trends in land-cover dynamics in relation with the patterns in precipitation and streamflow. This late analytical step yields a holistic picture of the ongoing alterations in the Niger River Basin. Finally, I emphasize suggestions, valuable for a comprehensive water resources and environment management.

  20. Effects of human activities on the ecological processes of river biofilms in a highly urbanized river

    Science.gov (United States)

    Hung, R.; Li, M.

    2013-12-01

    Many anthropogenic disturbances and their effects of aquatic ecosystem are difficult to quantify in urbanized rivers. In past, specific taxa analysis of community structure was a common approach in river health monitoring studies. However, it is still difficult to understand stream ecosystem integrity without considering ecosystem processes. The complex species composition and metabolism of a river biofilm have the capacity to interact and/or modulate their surrounding environment. Because of their short life cycles, species richness, and worldwide distribution, structure and function of river biofilm communities are sensitive to change in environmental conditions. Therefore, biofilms are widely used as early warning systems of water pollution for water quality monitoring studies. In this study, we used river biofilms as a bioindicator by examining their extracellular enzyme activities and photosynthesis efficiency to understand human activities on the ecological processes of river ecosystem in a highly urbanized river. We sampled four sites along the Keelung River, Taiwan, based on different intensities of anthropogenic disturbances including water pollution index, population densities, land use types and types of stream habitats. Two study sites are heavily influenced by human activities and the others are not. The activities of extracellular enzymes within the biofilm play an important function for organic matter decomposition and nutrient cycling. We measured seven extracellular enzyme activities (β-d-glucosidase, phosphatase, leucine-aminopeptidase, sulfatase, peroxidase, polyphenol oxidase, and esterase) to examine specific enzyme activity changes at four study sites monthly. In addition, relative proportion of each extracellular enzyme activity on total enzyme activities was calculated in order to examine the relationship between functional biofilm profiles and different urban intensities. Among four study sites, leucine-aminopeptidase and esterase

  1. The Physical and Biochemical Alteration of the Platte River by Phragmites australis, an Invasive Species of Wetland Grass

    Science.gov (United States)

    Mohr, R. C.; Krueger, R.; Triplett, L.; Michal, T.; Kettenring, K. M.

    2014-12-01

    Invasive species can have a profound impact on the ecosystems to which they are introduced. Beginning in 2003, the Platte River, Nebraska, USA, was invaded by an aggressive species of wetland grass, Phragmites australis. The invasion by Phragmites, in combination with river flow reductions due to agricultural irrigation, has drastically altered the character and morphology of the river. Once a braided and largely unvegetated river, the Platte had become densely colonized with vegetation by 2010. We measured some physical and biochemical characteristics of Platte River sediments to infer how that vegetation has changed the system. Specifically, we measured particle size, which is an indicator of flow velocity, and biogenic silica (BSi), which is a critical source of silicon for some aquatic organisms. Sediment was collected from areas of the riverbed that are unvegetated, and from areas that are occupied by Phragmites or native vegetation. Particle size was measured using a laser diffractometer to determine how much fine particle deposition was occurring. Biogenic silica (BSi) concentrations were measured using timed NaOH digestions and inductively coupled plasma mass spectrometry (ICP-MS). Our results indicate that stands of Phragmites in the Platte River cause more deposition of finer silt-sized particles than other parts of the river that are unvegetated or are occupied by native vegetation. Also, Phragmites increased the sequestration of BSi in the river sediments. These changes to the Platte reverberate beyond the river itself; by sequestering silica in sediments, Phragmites could be diminishing the supply of silica to estuaries and coastal oceans. Hypothesizing that the silica content of the Platte's water had been reduced by the arrival of Phragmites, we measured dissolved (DSi) and biogenic silica (BSi) concentrations of Platte water using ICP-MS to compare to existing data from the 1990s.

  2. [Reasons of high concentration ammonium in Yellow River, China].

    Science.gov (United States)

    Zhang, Xue-qing; Xia, Xing-hui; Yang, Zhi-feng

    2007-07-01

    Ammonium nitrogen contamination is one of the major problems of the Yellow River in China. The speciation, concentration and sources of nitrogen compounds as well as the water environment conditions of the Yellow River had been analyzed to study the reasons for the fact that the ammonium nitrogen concentration was above the water quality standard. In addition, laboratory experiments had been carried out to investigate the effects of suspended sediment (SS) on nitrification rate. The results indicated that the presence of SS could accelerate the nitrification process, therefore, the effects of SS on nitrification rate was not the reason for the high level of ammonium nitrogen in the river. The excessive and continuous input of nitrogen contaminants to the river was the fundamental reason for the high concentration of ammonium nitrogen. Organic and ammonium nitrogen with high concentration inhibitted the nitrification processes. When the initial NH4+ -N concentrations were 10.1, 18.4 and 28.2 mg/L, nitrification efficiencies were 17.4%, 13.0% and 2.5%, respectively. When the initial organic nitrogen concentrations were 5.5 and 8.6 mg/L, the maximum concentrations of ammonium nitrogen produced by the oxidation of organic nitrogen would reach 0.47 and 1.69 mg/L and they would last for 2 days and 6 days, respectively. The oxygen-consuming organics and toxic substance existing in the river water could inhibit the activity of nitrifying bacteria, and thus lead to the accumulation of ammonium nitrogen. In addition, the high pH value of river water resulted in the high concentration of nonionic ammonium nitrogen which would reduce the activity of nitrifying bacteria and decrease the nitrification rates. Besides, low river runoff, low SS content and low activity of nitrifying bacteria resulted in the high level of ammonium nitrogen of the river in the low water season.

  3. Methane Emissions from a Hydrologically Altered Region of the Sprague River Salt Marsh, Phippsburg, ME

    Science.gov (United States)

    Cohen-Kaplan, Dana; Johnson, Beverly; Dostie, Phil; Carey, Joanna

    2016-04-01

    Humans have been altering salt marshes for 200 years, by ditching, or excavating channels to drain pools to reduce mosquito habitat and to make the marsh platform suitable for agriculture. The presence of these ditches has had a negative impact on the marsh hydrology. The Sprague River Salt Marsh, located in Phippsburg, ME, has been ditched for centuries. In 2002, the United States Fish and Wildlife Service installed several ditch-plugs in the marsh in and attempt to restore pool habitat and marsh hydrology. This study seeks to examine the effects of one ditch-plug in particular, and the impact it has had on methane emissions, soil salinity, and soil carbon densities. Three sites above and three sites below the ditch-plug were analyzed in these three areas. Methane emissions were slightly elevated above the ditch-plug relative to below the ditch-plug, with average fluxes of 12.9 +/- 4.83 umol/hr/m2 and 3.5 +/- 0.66 umol/hr/m^2, respectively. Soil salinities were higher above the ditch-plug, and soil carbon densities were higher below the ditch-plug. These results suggest that the hydrologic regime of the study area has been degraded, resulting in methane emissions, interrupted accretion, decreased marsh self-maintenance processes, stagnation and saturation of seawater in the pores of the sediment. Though salt marshes are generally considered effective carbon sinks, methane emissions may play a role on hydrologically altered marshes in terms of their net contribution to greenhouse gas sequestration.

  4. Impact of hydrological alterations on river-groundwater exchange and water quality in a semi-arid area: Nueces River, Texas.

    Science.gov (United States)

    Murgulet, Dorina; Murgulet, Valeriu; Spalt, Nicholas; Douglas, Audrey; Hay, Richard G

    2016-12-01

    There is a lack of understanding and methods for assessing the effects of anthropogenic disruptions, (i.e. river fragmentation due to dam construction) on the extent and degree of groundwater-surface water interaction and geochemical processes affecting the quality of water in semi-arid, coastal catchments. This study applied a novel combination of electrical resistivity tomography (ERT) and elemental and isotope geochemistry in a coastal river disturbed by extended drought and periodic flooding due to the operation of multiple dams. Geochemical analyses show that the saltwater barrier causes an increase in salinity in surface water in the downstream river as a result of limited freshwater inflows, strong evaporation effects on shallow groundwater and mostly stagnant river water, and is not due to saltwater intrusion by tidal flooding. Discharge from bank storage is dominant (~84%) in the downstream fragment and its contribution could increase salinity levels within the hyporheic zone and surface water. When surface water levels go up due to upstream freshwater releases the river temporarily displaces high salinity water trapped in the hyporheic zone to the underlying aquifer. Geochemical modeling shows a higher contribution of distant and deeper groundwater (~40%) in the upstream river and lower discharge from bank storage (~13%) through the hyporheic zone. Recharge from bank storage is a source of high salt to both upstream and downstream portions of the river but its contribution is higher below the dam. Continuous ERT imaging of the river bed complements geochemistry findings and indicate that while lithologically similar, downstream of the dam, the shallow aquifer is affected by salinization while fresher water saturates the aquifer in the upstream fragment. The relative contribution of flows (i.e. surface water releases or groundwater discharge) as related to the river fragmentation control changes of streamwater chemistry and likely impact the interpretation

  5. Combined use of stable isotopes and hydrologic modeling to better understand nutrient sources and cycling in highly altered systems (Invited)

    Science.gov (United States)

    Young, M. B.; Kendall, C.; Guerin, M.; Stringfellow, W. T.; Silva, S. R.; Harter, T.; Parker, A.

    2013-12-01

    The Sacramento and San Joaquin Rivers provide the majority of freshwater for the San Francisco Bay Delta. Both rivers are important sources of drinking and irrigation water for California, and play critical roles in the health of California fisheries. Understanding the factors controlling water quality and primary productivity in these rivers and the Delta is essential for making sound economic and environmental water management decisions. However, these highly altered surface water systems present many challenges for water quality monitoring studies due to factors such as multiple potential nutrient and contaminant inputs, dynamic source water inputs, and changing flow regimes controlled by both natural and engineered conditions. The watersheds for both rivers contain areas of intensive agriculture along with many other land uses, and the Sacramento River receives significant amounts of treated wastewater from the large population around the City of Sacramento. We have used a multi-isotope approach combined with mass balance and hydrodynamic modeling in order to better understand the dominant nutrient sources for each of these rivers, and to track nutrient sources and cycling within the complex Delta region around the confluence of the rivers. High nitrate concentrations within the San Joaquin River fuel summer algal blooms, contributing to low dissolved oxygen conditions. High δ15N-NO3 values combined with the high nitrate concentrations suggest that animal manure is a significant source of nitrate to the San Joaquin River. In contrast, the Sacramento River has lower nitrate concentrations but elevated ammonium concentrations from wastewater discharge. Downstream nitrification of the ammonium can be clearly traced using δ15N-NH4. Flow conditions for these rivers and the Delta have strong seasonal and inter-annual variations, resulting in significant changes in nutrient delivery and cycling. Isotopic measurements and estimates of source water contributions

  6. Alteration of Water Pollution Level with the Seasonal Changes in Mean Daily Discharge in Three Main Rivers around Dhaka City, Bangladesh

    Directory of Open Access Journals (Sweden)

    Md. Saiful Islam

    2015-06-01

    Full Text Available A study based on the physicochemical parameters and dissolved metals levels from three main rivers around Dhaka City, Bangladesh, was conducted in order to determine the present pollution status and their alteration trends with the seasonal change of discharge amount. The water samples were collected from the rivers Buriganga, Turag, and Shitalakkhya during both dry and monsoon seasons. Physicochemical analyses revealed that most of the water quality parameters exceeded the recommended levels set by the Department of Environment (DoE, Bangladesh, during both the dry and monsoon seasons. A very strong positive correlation was found between biochemical oxygen demand (BOD and chemical oxygen demand (COD in all sampling points. Both BOD and COD values had a strong negative correlation with dissolved oxygen (DO in the Shitalakkhya River. Most of the dissolved metals concentrations in the water samples were similar. However, the concentrations of different physicochemical properties varied with the seasons. The dry season had significantly higher contamination loads, which were decreased during the monsoon season. Anthropogenic activities, as well as the variation in river water flow during different seasons were the main reasons for this high degree of water pollution.

  7. Water Temperature Dynamics in High Arctic River Basins

    Science.gov (United States)

    Blaen, P. J.; Hannah, D. M.; Brown, L. E.; Milner, A. M.

    2012-04-01

    Despite the high sensitivity of polar regions to climate change, and the strong influence of temperature upon ecosystem processes, contemporary understanding of water temperature dynamics in Arctic river systems is limited. This research gap is addressed by exploring high-resolution water column thermal regimes for glacier-fed and non-glacial rivers at eight sites across Svalbard during the 2010 melt season. Mean water column temperatures in glacier-fed rivers (0.3 - 3.2 °C) were the lowest and most thermally-stable near the glacier terminus but increased downstream (0.7 - 2.3 °C km-1). Non-glacial rivers, where discharge was sourced primarily from snowmelt, were warmer (mean 2.9 - 5.7 °C) and more variable, indicating increased water residence times in shallow alluvial zones with increased potential for atmospheric influence. Mean summer water temperature and the magnitude of daily thermal variation were similar to those of Alaskan rivers but low at all sites when compared to alpine glacierized environments at lower latitudes. Thermal regimes were strongly correlated (pgeomorphological features (e.g. channel morphology). These results provide insight into the potential changes in high-latitude river systems in the context of projected warming in polar regions. We hypothesise warmer and more variable temperature regimes may prevail in future as the proportion of bulk discharge sourced from glacial meltwater declines and rivers undergo a progressive shift towards snow- and groundwater sources. Importantly, such changes could have implications for species diversity and abundance in benthic communities and influence rates of ecosystem functioning in high-latitude aquatic systems.

  8. Two dimensional hydrodynamic modeling of a high latitude braided river

    Science.gov (United States)

    Humphries, E.; Pavelsky, T.; Bates, P. D.

    2014-12-01

    Rivers are a fundamental resource to physical, ecologic and human systems, yet quantification of river flow in high-latitude environments remains limited due to the prevalence of complex morphologies, remote locations and sparse in situ monitoring equipment. Advances in hydrodynamic modeling and remote sensing technology allow us to address questions such as: How well can two-dimensional models simulate a flood wave in a highly 3-dimensional braided river environment, and how does the structure of such a flood wave differ from flow down a similar-sized single-channel river? Here, we use the raster-based hydrodynamic model LISFLOOD-FP to simulate flood waves, discharge, water surface height, and velocity measurements over a ~70 km reach of the Tanana River in Alaska. In order to use LISFLOOD-FP a digital elevation model (DEM) fused with detailed bathymetric data is required. During summer 2013, we surveyed 220,000 bathymetric points along the study reach using an echo sounder system connected to a high-precision GPS unit. The measurements are interpolated to a smooth bathymetric surface, using Topo to Raster interpolation, and combined with an existing five meter DEM (Alaska IfSAR) to create a seamless river terrain model. Flood waves are simulated using varying complexities in model solvers, then compared to gauge records and water logger data to assess major sources of model uncertainty. Velocity and flow direction maps are also assessed and quantified for detailed analysis of braided channel flow. The most accurate model output occurs with using the full two-dimensional model structure, and major inaccuracies appear to be related to DEM quality and roughness values. Future work will intercompare model outputs with extensive ground measurements and new data from AirSWOT, an airborne analog for the Surface Water and Ocean Topography (SWOT) mission, which aims to provide high-resolution measurements of terrestrial and ocean water surface elevations globally.

  9. Three Gorges Dam alters the Changjiang (Yangtze) river water cycle in the dry seasons: Evidence from H-O isotopes.

    Science.gov (United States)

    Deng, Kai; Yang, Shouye; Lian, Ergang; Li, Chao; Yang, Chengfan; Wei, Hailun

    2016-08-15

    As the largest hydropower project in the world, the Three Gorges Dam (TGD) has attracted great concerns in terms of its impact on the Changjiang (Yangtze) River and coastal marine environments. In this study, we measured or collected the H-O isotopic data of river water, groundwater and precipitation in the mid-lower Changjiang catchment during the dry seasons of recent years. The aim was to investigate the changes of river water cycle in response to the impoundment of the TGD. Isotopic evidences suggested that the mid-lower Changjiang river water was ultimately derived from precipitation, but dominated by the mixing of different water masses with variable sources and isotopic signals as well. The isotopic parameter "deuterium excess" (d-excess) yielded large fluctuations along the mid-lower mainstream during the initial stage of the TGD impoundment, which was inherited from the upstream water with inhomogeneous isotopic signals. However, as the reservoir water level rising to the present stage, small variability of d-excess was observed along the mid-lower mainstream. This discrepancy could be explained that the TGD impoundment had significantly altered the water cycle downstream the dam, with the rising water level increasing the residence time and enhancing the mixing of reservoir water derived from upstream. This eventually resulted in the homogenization of reservoir water, and thus small fluctuations of d-excess downstream the dam after the quasi-normal stage (2008 to present). We infer that the retention effect of large reservoirs has greatly buffered the d-excess natural variability of water cycle in large river systems. Nevertheless, more research attention has to be paid to the damming effect on the water cycle in the river, estuarine and coastal areas, especially during the dry seasons.

  10. Simulating Spawning and Juvenile Rainbow Trout (Oncorhynchus mykiss Habitat in Colorado River Based on High-Flow Effects

    Directory of Open Access Journals (Sweden)

    Weiwei Yao

    2017-02-01

    Full Text Available High flow generates significant alterations in downstream river reaches, resulting in physical condition changes in the downstream regions of the river such as water depth, flow velocity, water temperature and river bed. These alterations will lead to change in fish habitat configuration in the river. This paper proposes a model system to evaluate the high flow effects on river velocity, water depth, substrates changes, temperature distribution and consequently assess the change in spawning and juvenile rainbow trout (Oncorhynchus mykiss habitats in the downstream region of the Glen Canyon Dam. Firstly, based on the 2 dimensional (2D depth-averaged CFD (Computational Fluid Dynamics model and heat transfer equation applied for simulation, three indices were simulated, namely depth, flow velocity and temperature distribution. Then, the spawning and juvenile fish preference curves were obtained based on these three indices and substrates distribution. After that, the habitat model was proposed and used to simulate the high flow effects on juvenile and spawning rainbow trout habitat structure. Finally, the weighted usable area (WUA and overall suitability index (OSI of the spawning and juvenile fish species were quantitatively simulated to estimate the habitat sensitivity. The results illustrate that the high flow effect (HFE increased the juvenile rainbow trout habitat quality but decreased the spawning rainbow trout habitat quality. The juvenile trout were mainly affected by the water depth while the spawning rainbow trout were dominated by the bed elevation.

  11. High-fat diet alters gut microbiota physiology in mice.

    Science.gov (United States)

    Daniel, Hannelore; Gholami, Amin Moghaddas; Berry, David; Desmarchelier, Charles; Hahne, Hannes; Loh, Gunnar; Mondot, Stanislas; Lepage, Patricia; Rothballer, Michael; Walker, Alesia; Böhm, Christoph; Wenning, Mareike; Wagner, Michael; Blaut, Michael; Schmitt-Kopplin, Philippe; Kuster, Bernhard; Haller, Dirk; Clavel, Thomas

    2014-02-01

    The intestinal microbiota is known to regulate host energy homeostasis and can be influenced by high-calorie diets. However, changes affecting the ecosystem at the functional level are still not well characterized. We measured shifts in cecal bacterial communities in mice fed a carbohydrate or high-fat (HF) diet for 12 weeks at the level of the following: (i) diversity and taxa distribution by high-throughput 16S ribosomal RNA gene sequencing; (ii) bulk and single-cell chemical composition by Fourier-transform infrared- (FT-IR) and Raman micro-spectroscopy and (iii) metaproteome and metabolome via high-resolution mass spectrometry. High-fat diet caused shifts in the diversity of dominant gut bacteria and altered the proportion of Ruminococcaceae (decrease) and Rikenellaceae (increase). FT-IR spectroscopy revealed that the impact of the diet on cecal chemical fingerprints is greater than the impact of microbiota composition. Diet-driven changes in biochemical fingerprints of members of the Bacteroidales and Lachnospiraceae were also observed at the level of single cells, indicating that there were distinct differences in cellular composition of dominant phylotypes under different diets. Metaproteome and metabolome analyses based on the occurrence of 1760 bacterial proteins and 86 annotated metabolites revealed distinct HF diet-specific profiles. Alteration of hormonal and anti-microbial networks, bile acid and bilirubin metabolism and shifts towards amino acid and simple sugars metabolism were observed. We conclude that a HF diet markedly affects the gut bacterial ecosystem at the functional level.

  12. Altered River Morphology in South Africa Related to the Permian-Triassic Extinction

    National Research Council Canada - National Science Library

    Peter D. Ward; David R. Montgomery; Roger Smith

    2000-01-01

    The Permian-Triassic transition in the Karoo Basin of South Africa was characterized by a rapid and apparently basin-wide change from meandering to braided river systems, as evidenced by preserved sedimentary facies...

  13. A preliminary study of older hot spring alteration in Sevenmile Hole, Grand Canyon of the Yellowstone River, Yellowstone Caldera, Wyoming

    Science.gov (United States)

    Larson, Peter B.; Phillips, Allison; John, David A.; Cosca, Michael A.; Pritchard, Chad; Andersen, Allen; Manion, Jennifer

    2009-01-01

    Erosion in the Grand Canyon of the Yellowstone River, Yellowstone Caldera (640 ka), Wyoming, has exposed a cross section of older hydrothermal alteration in the canyon walls. The altered outcrops of the post-collapse tuff of Sulphur Creek (480 ka) extend from the canyon rim to more than 300 m beneath it. The hydrothermal minerals are zoned, with an advanced argillic alteration consisting of an association of quartz (opal) + kaolinite ± alunite ± dickite, and an argillic or potassic alteration association with quartz + illite ± adularia. Disseminated fine-grained pyrite or marcasite is ubiquitous in both alteration types. These alteration associations are characteristic products of shallow volcanic epithermal environments. The contact between the two alteration types is about 100 m beneath the rim. By analogy to other active geothermal systems including active hydrothermal springs in the Yellowstone Caldera, the transition from kaolinite to illite occurred at temperatures in the range 150 to 170 °C. An 40Ar/39Ar age on alunite of 154,000 ± 16,000 years suggests that hydrothermal activity has been ongoing since at least that time. A northwest-trending linear array of extinct and active hot spring centers in the Sevenmile Hole area implies a deeper structural control for the upflowing hydrothermal fluids. We interpret this deeper structure to be the Yellowstone Caldera ring fault that is covered by the younger tuff of Sulphur Creek. The Sevenmile Hole altered area lies at the eastern end of a band of hydrothermal centers that may mark the buried extension of the Yellowstone Caldera ring fault across the northern part of the Caldera.

  14. Structural and ecophysiological alterations of the water hyacinth [Eichhornia crassipes (Mart. Solms] due to anthropogenic stress in Brazilian rivers

    Directory of Open Access Journals (Sweden)

    Angela Pierre Vitória

    2011-10-01

    Full Text Available In this work, the structural and ecophysiological alterations (chlorophyll a fluorescence and photosynthetic pigments, and quantification of Cr, Pb and Zn in the leaf limb, petiole and younger and older roots of water hyacinth from the lower, medium and upper Paraíba do Sul river (PSR and Imbé river were evaluated. The plants from the medium and upper PSR (more industrialized and populated regions exhibited lower turgid cell in the root cortex, less root hairs and leaf epidermis, chloroplasts with plastoglobules and increased stroma volume. Higher concentrations of metals were observed in the younger and older roots from the medium PSR plants. The results suggested that the plants from more anthropized regions were able to maintain the maximum quantum yield (Fv/Fm which was a result from the metabolic fitting, increasing the non-photochemical quenching, reducing total chlorophyll/carotenoids and leading to the structural modifications.

  15. A highly specific coding system for structural chromosomal alterations.

    Science.gov (United States)

    Martínez-Frías, M L; Martínez-Fernández, M L

    2013-04-01

    The Spanish Collaborative Study of Congenital Malformations (ECEMC, from the name in Spanish) has developed a very simple and highly specific coding system for structural chromosomal alterations. Such a coding system would be of value at present due to the dramatic increase in the diagnosis of submicroscopic chromosomal deletions and duplications through molecular techniques. In summary, our new coding system allows the characterization of: (a) the type of structural anomaly; (b) the chromosome affected; (c) if the alteration affects the short or/and the long arm, and (d) if it is a non-pure dicentric, a non-pure isochromosome, or if it affects several chromosomes. We show the distribution of 276 newborn patients with these types of chromosomal alterations using their corresponding codes according to our system. We consider that our approach may be useful not only for other registries, but also for laboratories performing these studies to store their results on case series. Therefore, the aim of this article is to describe this coding system and to offer the opportunity for this coding to be applied by others. Moreover, as this is a SYSTEM, rather than a fixed code, it can be implemented with the necessary modifications to include the specific objectives of each program. Copyright © 2013 Wiley Periodicals, Inc.

  16. Three Gorges Dam alters the Changjiang (Yangtze) river water cycle in the dry seasons: Evidence from H-O isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Deng, Kai [School of Ocean and Earth Science, Tongji University, Shanghai, 200092 (China); State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092 (China); Yang, Shouye, E-mail: syyang@tongji.edu.cn [State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092 (China); Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266061 (China); Lian, Ergang [School of Ocean and Earth Science, Tongji University, Shanghai, 200092 (China); State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092 (China); Li, Chao [State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092 (China); Yang, Chengfan; Wei, Hailun [School of Ocean and Earth Science, Tongji University, Shanghai, 200092 (China); State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092 (China)

    2016-08-15

    As the largest hydropower project in the world, the Three Gorges Dam (TGD) has attracted great concerns in terms of its impact on the Changjiang (Yangtze) River and coastal marine environments. In this study, we measured or collected the H-O isotopic data of river water, groundwater and precipitation in the mid-lower Changjiang catchment during the dry seasons of recent years. The aim was to investigate the changes of river water cycle in response to the impoundment of the TGD. Isotopic evidences suggested that the mid-lower Changjiang river water was ultimately derived from precipitation, but dominated by the mixing of different water masses with variable sources and isotopic signals as well. The isotopic parameter “deuterium excess” (d-excess) yielded large fluctuations along the mid-lower mainstream during the initial stage of the TGD impoundment, which was inherited from the upstream water with inhomogeneous isotopic signals. However, as the reservoir water level rising to the present stage, small variability of d-excess was observed along the mid-lower mainstream. This discrepancy could be explained that the TGD impoundment had significantly altered the water cycle downstream the dam, with the rising water level increasing the residence time and enhancing the mixing of reservoir water derived from upstream. This eventually resulted in the homogenization of reservoir water, and thus small fluctuations of d-excess downstream the dam after the quasi-normal stage (2008 to present). We infer that the retention effect of large reservoirs has greatly buffered the d-excess natural variability of water cycle in large river systems. Nevertheless, more research attention has to be paid to the damming effect on the water cycle in the river, estuarine and coastal areas, especially during the dry seasons. - Highlights: • Stable H-O isotopes indicate the Changjiang river water cycle in dry seasons. • The isotopic parameter “d-excess” reveals the origins of

  17. Setting Preferences of High and Low Use River Recreationists: How Different are They?

    Science.gov (United States)

    Kainzinger, Silvia; Arnberger, Arne; Burns, Robert C.

    2016-11-01

    Whitewater boaters often choose a river based on their preferences for attributes important for their trip experience. This study explored whether preferences and tradeoffs of whitewater boaters for social, resource, and managerial attributes of riverscapes differ among a high and a low use river in the United States by employing a stated choice approach. River trip scenarios were displayed using verbal descriptions and computer-generated photographs. Results indicate that use levels were more important for boaters on the low use river, whereas river difficulty and river access fee was of higher importance for the high use river boaters, who are more involved in this whitewater activity. Preferences for waiting times and trip length did not differ between the samples. Findings suggest that whitewater boaters of high and low use rivers have a different tradeoff behavior among river setting attributes, which has implications for river recreation management.

  18. Source identification of high glyme concentrations in the Oder River.

    Science.gov (United States)

    Stepien, D K; Püttmann, W

    2014-05-01

    The objective of the following study was to identify the source of high concentrations of glycol diethers (diglyme, triglyme, and tetraglyme) in the Oder River. Altogether four sampling campaigns were conducted and over 50 surface samples collected. During the first two samplings of the Oder River in the Oderbruch region (km 626-690), glymes were detected at concentrations reaching 0.065 μg L(-1) (diglyme), 0.54 μg L(-1) (triglyme) and 1.7 μg L(-1) (tetraglyme). The subsequent sampling of the Oder River, from the area close to the source to the Poland-Germany border (about 500 km) helped to identify the possible area of the dominating glyme entry into the river between km 310 and km 331. During that sampling, the maximum concentration of triglyme was 0.46 μg L(-1) and tetraglyme 2.2 μg L(-1); diglyme was not detected. The final sampling focused on the previously identified area of glyme entry, as well as on tributaries of the Oder River. Samples from Czarna Woda stream and Kaczawa River contained even higher concentrations of diglyme, triglyme, and tetraglyme, reaching 5.2 μg L(-1), 13 μg L(-1) and 81 μg L(-1), respectively. Finally, three water samples were analyzed from a wastewater treatment plant receiving influents from a Copper Smelter and Refinery; diglyme, triglyme, and tetraglyme were present at a maximum concentration of 1700 μg L(-1), 13,000 μg L(-1), and 190,000 μg L(-1), respectively. Further research helped to identify the source of glymes in the wastewater. The gas desulfurization process Solinox uses a mixture of glymes (Genosorb(®)1900) as a physical absorption medium to remove sulfur dioxide from off-gases from the power plant. The wastewater generated from the process and from the maintenance of the equipment is initially directed to the wastewater treatment plant where it undergoes mechanical and chemical treatment processes before being discharged to the tributaries of the Oder River. Although monoglyme was

  19. (238)U/(235)U isotope ratios of crustal material, rivers and products of hydrothermal alteration: new insights on the oceanic U isotope mass balance.

    Science.gov (United States)

    Noordmann, Janine; Weyer, Stefan; Georg, R Bastian; Jöns, Svenja; Sharma, Mukul

    2016-01-01

    In this study, the U isotope composition, n((238)U)/n((235)U), of major components of the upper continental crust, including granitic rocks of different age and post-Archaean shales, as well as that of rivers (the major U source to the oceans) was investigated. Furthermore, U isotope fractionation during the removal of U at mid-ocean ridges, an important sink for U from the oceans, was investigated by the analyses of hydrothermal water samples (including low- and high-temperature fluids), low-temperature altered basalts and calcium carbonate veins. All analysed rock samples from the continental crust fall into a limited range of δ(238)U between -0.45 and -0.21 ‰ (relative to NBL CRM 112-A), with an average of -0.30 ± 0.15 ‰ (2 SD, N = 11). Despite differences in catchment lithologies, all major rivers define a relatively narrow range between -0.31 and -0.13 ‰, with a weighted mean isotope composition of -0.27 ‰, which is indistinguishable from the estimate for the upper continental crust (-0.30 ‰). Only some tributary rivers from the Swiss Alps display a slightly larger range in δ(238)U (-0.29 to +0.01 ‰) and lower U concentrations (0.87-3.08 nmol/kg) compared to the investigated major rivers (5.19-11.69 nmol/kg). These findings indicate that only minor net U isotope fractionation occurs during weathering and transport of material from the continental crust to the oceans. Altered basalts display moderately enriched U concentrations (by a factor of 3-18) compared to those typically observed for normal mid-ocean ridge basalts. These, and carbonate veins within altered basalts, show large U isotope fractionation towards both heavy and light U isotope compositions (ranging from -0.63 to +0.27 ‰). Hydrothermal water samples display low U concentrations (0.3-1 nmol/kg) and only limited variations in their U isotope composition (-0.43 ± 0.25 ‰) around the seawater value. Nevertheless, two of the investigated fluids display

  20. Exposing native cyprinid (Barbus plebejus) juveniles to river sediments leads to gonadal alterations, genotoxic effects and thyroid disruption.

    Science.gov (United States)

    Viganò, Luigi; De Flora, Silvio; Gobbi, Marco; Guiso, Giovanna; Izzotti, Alberto; Mandich, Alberta; Mascolo, Giuseppe; Roscioli, Claudio

    2015-12-01

    Juveniles (50 days post hatch) of a native cyprinid fish (Barbus plebejus) were exposed for 7 months to sediments from the River Lambro, a polluted tributary impairing the quality of the River Po for tens of kilometers from their confluence. Sediments were collected upstream of the city of Milan and downstream at the closure of the drainage basin of the River Lambro. Chemical analyses revealed the presence of a complex mixture of bioavailable endocrine-active chemicals, with higher exposure levels in the downstream section of the tributary. Mainly characterized by brominated flame retardants, alkylphenols, polychlorinated biphenyls, and minor co-occurring personal care products and natural hormones, the sediment contamination induced reproductive disorders, as well as other forms of endocrine disruption and toxicity. In particular, exposed male barbel exhibited higher biliary PAH-like metabolites, overexpression of the cyp1a gene, vitellogenin production in all specimens, the presence of oocytes (up to 22% intersex), degenerative alterations in their testis, liver fat vacuolization, a marked depression of total thyroxine (T4) and triiodothyronine (T3) plasma levels, and genotoxic damages determined as hepatic DNA adducts. These results clearly demonstrate that Lambro sediments alone are responsible for recognizable changes in the structure and function of the reproductive and, in general, the endocrine system of a native fish species. In the real environment, exposure to waterborne and food-web sources of chemicals are responsible for additional toxic loads, and the present findings thus provide evidence for a causal role of this tributary in the severe decline observed in barbel in recent decades and raise concern that the fish community of the River Po is exposed to endocrine-mediated health effects along tens of kilometres of its course.

  1. The alterations in high density polyethylene properties with gamma irradiation

    Science.gov (United States)

    Zaki, M. F.; Elshaer, Y. H.; Taha, Doaa. H.

    2017-10-01

    In the present investigation, high density polyethylene (HDPE) polymer has been used to study the alterations in its properties under gamma-irradiation. Physico-chemical properties have been investigated with different spectroscopy techniques, Fourier Transform Infrared spectroscopy (FTIR), X-ray diffraction (XRD), biocompatibility properties, as well as, mechanical properties change. The FT-IR analysis shows the formation of new band at 1716 cm-1 that is attributed to the oxidation of irradiated polymer chains, which is due to the formation of carbonyl groups (C˭O). XRD patterns show that a decrease in the crystallite size and increase in the Full Width at Half Maximum (FWHM). This means that the crystallinity of irradiated samples is decreased with increase in gamma dose. The contact angle measurements show an increase in the surface free energy as the gamma irradiation increases. The measurements of mechanical properties of irradiated HDPE samples were discussed.

  2. Altered river morphology in south africa related to the permian-triassic extinction

    Science.gov (United States)

    Ward; Montgomery; Smith

    2000-09-08

    The Permian-Triassic transition in the Karoo Basin of South Africa was characterized by a rapid and apparently basin-wide change from meandering to braided river systems, as evidenced by preserved sedimentary facies. This radical changeover in river morphology is consistent with geomorphic consequences stemming from a rapid and major die-off of rooted plant life in the basin. Evidence from correlative nonmarine strata elsewhere in the world containing fluvial Permian-Triassic boundary sections suggests that a catastrophic terrestrial die-off of vegetation was a global event, producing a marked increase in sediment yield as well as contributing to the global delta(13)C excursion across the Permian-Triassic boundary.

  3. Castration alters protein balance after high-frequency muscle contraction.

    Science.gov (United States)

    Steiner, Jennifer L; Fukuda, David H; Rossetti, Michael L; Hoffman, Jay R; Gordon, Bradley S

    2017-02-01

    Resistance exercise increases muscle mass by shifting protein balance in favor of protein accretion. Androgens independently alter protein balance, but it is unknown whether androgens alter this measure after resistance exercise. To answer this, male mice were subjected to sham or castration surgery 7-8 wk before undergoing a bout of unilateral, high-frequency, electrically induced muscle contractions in the fasted or refed state. Puromycin was injected 30 min before euthanasia to measure protein synthesis. The tibialis anterior was analyzed 4 h postcontraction. In fasted mice, neither basal nor stimulated rates of protein synthesis were affected by castration despite lower phosphorylation of mechanistic target of rapamycin in complex 1 (mTORC1) substrates [p70S6K1 (Thr389) and 4E-BP1 (Ser65)]. Markers of autophagy (LC3 II/I ratio and p62 protein content) were elevated by castration, and these measures remained elevated above sham values after contractions. Furthermore, in fasted mice, the protein content of Regulated in Development and DNA Damage 1 (REDD1) was correlated with LC3 II/I in noncontracted muscle, whereas phosphorylation of uncoordinated like kinase 1 (ULK1) (Ser757) was correlated with LC3 II/I in the contracted muscle. When mice were refed before contractions, protein synthesis and mTORC1 signaling were not affected by castration in either the noncontracted or contracted muscle. Conversely, markers of autophagy remained elevated in the muscles of refed, castrated mice even after contractions. These data suggest the castration-mediated elevation in baseline autophagy reduces the absolute positive shift in protein balance after muscle contractions in the refed or fasted states.

  4. TREHS (Temporary Rivers Ecological and Hydrological Status): new software for investigating the degree of hydrologic alteration of temporary streams.

    Science.gov (United States)

    Gallart, Francesc; Llorens, Pilar; Cid, Núria; latron, Jérôme; Bonada, Núria; Prat, Narcís

    2017-04-01

    individuals, and by interpretation of aerial photographs. Up to six metrics defining the permanence of water flow, the presence of stagnant pools and their temporal patterns of occurrence are used to determine the natural and observed river regime, and to assess the degree of hydrological alteration. Here, given the lack of agreed standards to evaluate the ecological relevance of the observed alterations, the thresholds that define quality class boundaries are provisional and may be updated using expert knowledge. Finally, the software characterizes the differences between the natural and actual regimes, performs a diagnosis of the hydrological status (degree of hydrologic alteration) along with an assessment of the significance and robustness of the diagnosis, and recommends the best period for biological quality samplings.

  5. Elk herbivory alters small mammal assemblages in high elevation drainages

    Science.gov (United States)

    Parsons, Elliott W.R.; Maron, John L.; Martin, Thomas E.

    2012-01-01

    Heavy herbivory by ungulates can substantially alter habitat, but the indirect consequences of habitat modification for animal assemblages that rely on that habitat are not well studied. This is a particularly important topic given that climate change can alter plant–herbivore interactions.

  6. Massive accumulation of highly polluted sedimentary deposits by river damming

    Energy Technology Data Exchange (ETDEWEB)

    Palanques, Albert, E-mail: albertp@icm.csic.es [Institute of Marine Sciences (CSIC), Passeig Maritim de la Barceloneta, 37-49, Barcelona 08003 (Spain); Grimalt, Joan [Institute of Environmental Assessment and Water Research (CSIC), Jordi Girona, 18, Barcelona 08034 (Spain); Belzunces, Marc; Estrada, Ferran; Puig, Pere; Guillén, Jorge [Institute of Marine Sciences (CSIC), Passeig Maritim de la Barceloneta, 37-49, Barcelona 08003 (Spain)

    2014-11-01

    Uncontrolled dumping of anthropogenic waste in rivers regulated by dams has created contaminated deposits in reservoirs that have remained unidentified for decades. The Flix Reservoir is located in the Ebro River, the second largest river flowing into the NW Mediterranean, has been affected by residue dumping from a chlor-alkali electrochemical plant for decades. High-resolution seismic profiles, bathymetric data, surficial sediment samples and sediment cores were obtained in the Flix Reservoir to study the characteristics of the deposit accumulated by this dumping. These data were used to reconstruct the waste deposit history. Since the construction of the Flix Dam in 1948, more than 3.6 × 10{sup 5} t of industrial waste has accumulated in the reservoir generating a delta-like deposit formed by three sediment lobes of fine-grained material highly contaminated by Hg, Cd, Zn and Cr (max: 640, 26, 420 and 750 mg kg{sup −1}, respectively). This contamination was associated with the Hg that was used for the cathode in the electrochemical plant from 1949 and with the production of phosphorite derivatives from 1973. After the construction of two large dams only a few kilometres upstream during the 1960s, the solids discharged from the industrial complex became the main sediment source to the Flix Reservoir. The deposit has remained in the reservoir forming a delta that obstructs about 50% of the river water section. Its stability only depended on the flow retention by the Flix Dam. At present, this contaminated waste deposit is being removed from the water reservoir as it is a cause of concern for the environment and for human health downriver. - Highlights: • A delta-like anthropogenic deposit prograded into the reservoir behind the Flix dam. • More than 3.6 × 10{sup 5} t of anthropogenic waste was accumulated in less than 4 decades. • A waste deposit with extreme levels of Hg and Cd was trapped in the Flix Reservoir. • The main pollution was related to

  7. MYOELECTRIC ALTERATIONS AFTER VOLUNTARY INDUCED HIGH - AND LOW - FREQUENCY FATIGUE

    Directory of Open Access Journals (Sweden)

    Vojko Strojnik

    2008-06-01

    Full Text Available The aim of the study was to find whether voluntary induced high- and low-frequency peripheral fatigue exhibit specific alteration in surface EMG signal (SEMG during evoked and maximum voluntary contractions. Ten male students of physical education performed 60 s long stretch-shortening cycle (SSC exercise with maximal intensity and 30 s long concentric (CON exercise with maximal intensity. To verify voluntary induced peripheral fatigue, knee torques during low- (T20 and high-frequency electrical stimulation (T100 of relaxed vastus lateralis muscle (VL were obtained. Contractile properties of the VL were measured with passive twitch and maximal voluntary knee extension test (MVC. Changes in M-waves and SEMG during MVC test were used to evaluate the differences in myoelectrical signals. T100/T20 ratio decreased by 10.9 ± 8.4 % (p < 0.01 after the SSC exercise and increased by 35.9 ± 17.5 % (p < 0.001 after the CON exercise. Significant SEMG changes were observed only after the CON exercise where peak to peak time of the M-waves increased by 9.2 ± 13.3 % (p < 0.06, SEMG amplitude during MVC increased by 32.9 ± 21.6 % (p < 0.001 and SEMG power spectrum median frequency decreased by 11.0 ± 10.5 % (p < 0.05. It is concluded that high frequency fatigue wasn't reflected in SEMG, however the SEMG changes after the CON seemed to reflect metabolic changes due to acidosis

  8. Interacting effects of discharge and channel morphology on transport of semibuoyant fish eggs in large, altered river systems.

    Directory of Open Access Journals (Sweden)

    Thomas A Worthington

    Full Text Available Habitat fragmentation and flow regulation are significant factors related to the decline and extinction of freshwater biota. Pelagic-broadcast spawning cyprinids require moving water and some length of unfragmented stream to complete their life cycle. However, it is unknown how discharge and habitat features interact at multiple spatial scales to alter the transport of semi-buoyant fish eggs. Our objective was to assess the relationship between downstream drift of semi-buoyant egg surrogates (gellan beads and discharge and habitat complexity. We quantified transport time of a known quantity of beads using 2-3 sampling devices at each of seven locations on the North Canadian and Canadian rivers. Transport time was assessed based on median capture time (time at which 50% of beads were captured and sampling period (time period when 2.5% and 97.5% of beads were captured. Habitat complexity was assessed by calculating width∶depth ratios at each site, and several habitat metrics determined using analyses of aerial photographs. Median time of egg capture was negatively correlated to site discharge. The temporal extent of the sampling period at each site was negatively correlated to both site discharge and habitat-patch dispersion. Our results highlight the role of discharge in driving transport times, but also indicate that higher dispersion of habitat patches relates to increased retention of beads within the river. These results could be used to target restoration activities or prioritize water use to create and maintain habitat complexity within large, fragmented river systems.

  9. Assessing inflow alterations into Lake Baikal from Selenga river basin with respect to changing climate and land use conditions

    Science.gov (United States)

    Moreido, Vsevolod; Kalugin, Andrey; Motovilov, Yuri; Millionshchikova, Tatiana

    2017-04-01

    The trans-boundary Selenga river basin is the largest tributary of Lake Baikal, which has been experiencing a profound increase of annual near-surface air temperature - 1.6 to 1.8⁰C during the last 70 years, nearly twice as global increase. A significant drought that has been registered in the Selenga basin for the last 20 years has drawn attention of a number of hydrologists worldwide to investigate its drivers. In this study we used the ECOMAG hydrological modeling software to construct a semi-distributed data-driven hydrological model that accounts for weather forcing, hydrological soil properties and land-use conditions to assess runoff generation in the river basin and its current and future response to climate and land-use alteration. Due to lack of daily weather observation data, the model was driven with EWEMBI weather dataset for calibration and validation purposes. To investigate the possible impact on runoff due to climate change in the XXI century we used an ensemble of 7 GCMs from CMIP5 experiment operating according to 4 IPCC greenhouse gas RCPs. To account for possible land-use changes we used HYDE dataset for the years 1990-2100. The modeling results show that the current drought conditions may be prolonged and intensified according to the most severe RCPs. At the same time, land-use conditions appear to have limited influence on the runoff generation for this area is not subject to heavy irrigation-intensive agriculture.

  10. Fish gills alterations as potential biomarkers of environmental quality in a eutrophized tropical river in south-eastern Brazil.

    Science.gov (United States)

    Nascimento, A A; Araújo, F G; Gomes, I D; Mendes, R M M; Sales, A

    2012-06-01

    Gill anomalies in three common fish species of different taxonomic order, habitat dwelling and feeding habits (one Characiformes, Oligosarcus hepsetus; one Siluriformes, Hypostomus auroguttatus; and one Perciformes, Geophagus brasiliensis) from a eutrophized tropical river in south-eastern in Brazil were compared. The aim of this study was to search for sentinel species that could be used as potential biomarkers of environmental quality. Most fish had gills with histological changes, namely epithelial lifting, interstitial oedema, leucocyte infiltration, hyperplasia of the epithelial cells, lamellar fusion, vasodilatation and necrosis. On the other hand, lamellar blood congestion and lamellar aneurysm, which are more serious and often irreversible changes, were recorded for the water column carnivorous O. hepsetus and, to a lesser extent, for the bottom-dwelling detritivorous H. auroguttatus. A histopathological alteration index (HAI) based on the occurrence and severity of gills anomalies indicated that O. hepsetus (mean score = 11.4) had significantly higher values (Kruskall-Wallis H(2,41) = 15.95, P = 0.0003) compared with G. brasiliensis (mean score = 7.0). Overall, the omnivorous G. brasiliensis had comparatively lesser occurrence of most gill anomalies compared with other two species, being less suitable as biomarker of environmental quality. In contrast, the water column-dweller O. hepsetus (water column) and the bottom-dweller H. auroguttatus had gills most susceptible to changes, making them more suitable for using as histological biomarkers of the environmental quality in entrophized tropical rivers.

  11. Hydrothermal alteration and melting of the crust during the Columbia River Basalt-Snake River Plain transition and the origin of low-δ18O rhyolites of the central Snake River Plain

    Science.gov (United States)

    Colón, Dylan P.; Bindeman, Ilya N.; Ellis, Ben S.; Schmitt, Axel K.; Fisher, Christopher M.

    2015-05-01

    We present compelling isotopic evidence from ~15 Ma rhyolites that erupted coeval with the Columbia River Basalts in southwest Idaho's J-P Desert and the Jarbidge Mountains of northern Nevada at that suggests that the Yellowstone mantle plume caused hydrothermal alteration and remelting of diverse compositions of shallow crust in the area where they erupted. These rhyolites also constitute the earliest known Miocene volcanism in the vicinity of the Bruneau-Jarbidge and Twin Falls (BJTF) volcanic complexes, a major center of voluminous (103-104 km3) low-δ18O rhyolitic volcanism that was previously defined as being active from 13 to 6 Ma. The Jarbidge Rhyolite has above-mantle δ18O (δ18O of +7.9‰ SMOW) and extremely unradiogenic εHf (- 34.7) and εNd (- 24.0). By contrast, the J-P Desert units are lower in δ18O (+4.5 to 5.8‰), and have more moderately unradiogenic whole-rock εHf (- 20.3 to - 8.9) and εNd (- 13.4 to - 7.7). The J-P Desert rhyolites are geochemically and petrologically similar to the younger rhyolites of the BJTF center (the one exception being their high δ18O values), suggesting a common origin for J-P Desert and BJTF rhyolites. The presence of low-δ18O values and unradiogenic Nd and Hf isotopic compositions, both of which differ greatly from the composition of a mantle differentiate, indicate that some of these melts may be 50% or more melted crust by volume. Individual J-P Desert units have isotopically diverse zircons, with one lava containing zircons ranging from - 0.6‰ to + 6.5‰ in δ18O and from - 29.5 to - 2.8 in εHf. Despite this diversity, zircons all have Miocene U/Pb ages. The range of zircon compositions fingerprints the diversity of their source melts, which in turn allow us to determine the compositions of two crustal end-members which melted to form these rhyolites. These end-members are: 1) Archean basement with normal to high-δ18O and unradiogenic εHf and 2) hydrothermally altered, shallow, young crust with low

  12. Altered neurotransmitter metabolism in adolescents with high-functioning autism

    NARCIS (Netherlands)

    Drenthen, G.S.; Barendse, E.M.; Aldenkamp, A.P.; Veenendaal, T. van; Puts, N.A.J.; Edden, R.A.E.; Zinger, S.; Thoonen, G.H.J.; Hendriks, M.P.H.; Kessels, R.P.C.; Jansen, J.F.A.

    2016-01-01

    Previous studies have suggested that alterations in excitatory/inhibitory neurotransmitters might play a crucial role in autism spectrum disorder (ASD). Proton magnetic resonance spectroscopy (1H-MRS) can provide valuable information about abnormal brain metabolism and neurotransmitter concentration

  13. Water quality assessment of highly polluted rivers in a semi-arid Mediterranean zone Oued Fez and Sebou River (Morocco)

    Science.gov (United States)

    Perrin, J. L.; Raïs, N.; Chahinian, N.; Moulin, P.; Ijjaali, M.

    2014-03-01

    Oued Fez (one of the Sebou River tributaries - Morocco) allowed us to study and quantify the effect of the lack of wastewater treatment on surface water quality in semi-arid hydrological context. The analysis is based on field data collected from June 2009 to December 2011. Concentration and load patterns of nitrogen, phosphorus and chromium (used in the processing of leather) are compared in stable hydrological conditions during low flow and high flow periods in an eight-location sampling network. The Oued Fez and the Sebou River are characterised by severe pollution downstream from the city of Fez, particularly TN (mainly NH4 and Norg), TP (mainly Ppart) and TCr. The most polluted sites are those directly under the influence of domestic and industrial waste water inputs, particularly tannery effluents. Obviously, the concentrations measured at these locations are above all environmental quality standards. Pollutant loads are very heavy in the Sebou River and can contaminate the river course for kilometres. Moreover, as the water of the Sebou River is used for the irrigation of vegetables, serious problems of public health could arise. A better understanding of contaminant dynamics and self-purifying processes in these rivers will help implement actions and steps aimed at improving water quality in the Sebou River, which is the primary water supply source in Morocco and is used for agricultural and industrials purposes as well as for drinking water.

  14. Hydraulic alterations resulting from hydropower development in the Bonneville Reach of the Columbia River

    Science.gov (United States)

    Hatten, James R.; Batt, Thomas R.

    2010-01-01

    We used a two-dimensional (2D) hydrodynamic model to simulate and compare the hydraulic characteristics in a 74-km reach of the Columbia River (the Bonneville Reach) before and after construction of Bonneville Dam. For hydrodynamic modeling, we created a bathymetric layer of the Bonneville Reach from single-beam and multi-beam echo-sounder surveys, digital elevation models, and navigation surveys. We calibrated the hydrodynamic model at 100 and 300 kcfs with a user-defined roughness layer, a variable-sized mesh, and a U.S. Army Corps of Engineers backwater curve. We verified the 2D model with acoustic Doppler current profiler (ADCP) data at 14 transects and three flows. The 2D model was 88% accurate for water depths, and 77% accurate for velocities. We verified a pre-dam 2D model run at 126 kcfs using pre-dam aerial photos from September 1935. Hydraulic simulations indicated that mean water depths in the Bonneville Reach increased by 34% following dam construction, while mean velocities decreased by 58%. There are numerous activities that would benefit from data output from the 2D model, including biological sampling, bioenergetics, and spatially explicit habitat modeling.

  15. Monitoring river morphological changes using high resolution multitemporal sar images: a case study on orco river, italy

    Science.gov (United States)

    Mitidieri, Francesco; Nicolina Papa, Maria; Ruello, Giuseppe; Amitrano, Donato; Bizzi, Simone; Demarchi, Luca

    2016-04-01

    Improving the knowledge about river processes by applying innovative monitoring techniques is extremely needed to face the challenge of a better river management. In this paper we test the capability of satellite synthetic aperture radar (SAR) images to enrich the monitoring of river geomorphological processes. Multitemporal SAR images provide observations and measurements at high spatial (3 m), and in particular temporal resolution (15 days). This information if properly processed and classified may significantly enrich our ability to monitor the evolution of river morphological phenomena (erosion/deposition, narrowing/widening, riparian vegetation's evolution and interferences with river flow). This is expected to lead to an enhancements in the river management capabilities, in particular as regards the assessment of hydro-morphological river quality, as strongly suggested by European Commission's Water Framework Directive (2000/60/EC). A case study on the Italian River Orco is here presented. The case study has used a set of 100 COSMO-SkyMed stripmap images (from October 2008 to November 2014) from Italian Space Agency. All the data were acquired with medium look angle (almost 30°) and HH polarization, also for increasing the land-water contrast. Calibration, registration and despeckling procedures were applied on the acquired dataset. In particular, the optimal weighting multitemporal De Grandi filter was adopted in order to allow an effective extraction of the water surfaces contour. This method was applied to extract water contours over the entire historical series of SAR datasets available. Thanks to the generated information we were able to monitor the lateral dynamic of the water channels and infer on the evolutions of erosion/deposition phenomena. To this aim, an RGB representation of multitemporal SAR data was implemented. The series of detected river channel morphological changes was then analyzed in the light of the series of discharge measurements in

  16. Riverbank filtration for the treatment of highly turbid Colombian rivers

    Science.gov (United States)

    Gutiérrez, Juan Pablo; van Halem, Doris; Rietveld, Luuk

    2017-05-01

    The poor quality of many Colombian surface waters forces us to seek alternative, sustainable treatment solutions with the ability to manage peak pollution events and to guarantee the uninterrupted provision of safe drinking water to the population. This review assesses the potential of using riverbank filtration (RBF) for the highly turbid and contaminated waters in Colombia, emphasizing water quality improvement and the influence of clogging by suspended solids. The suspended sediments may be favorable for the improvement of the water quality, but they may also reduce the production yield capacity. The cake layer must be balanced by scouring in order for an RBF system to be sustainable. The infiltration rate must remain high enough throughout the river-aquifer interface to provide the water quantity needed, and the residence time of the contaminants must be sufficient to ensure adequate water quality. In general, RBF seems to be a technology appropriate for use in highly turbid and contaminated surface rivers in Colombia, where improvements are expected due to the removal of turbidity, pathogens and to a lesser extent inorganics, organic matter and micro-pollutants. RBF has the potential to mitigate shock loads, thus leading to the prevention of shutdowns of surface water treatment plants. In addition, RBF, as an alternative pretreatment step, may provide an important reduction in chemical consumption, considerably simplifying the operation of the existing treatment processes. However, clogging and self-cleansing issues must be studied deeper in the context of these highly turbid waters to evaluate the potential loss of abstraction capacity yield as well as the development of different redox zones for efficient contaminant removal.

  17. Global change impacts on river ecosystems: A high-resolution watershed study of Ebro river metabolism.

    Science.gov (United States)

    Val, Jonatan; Chinarro, David; Pino, María Rosa; Navarro, Enrique

    2016-11-01

    Global change is transforming freshwater ecosystems, mainly through changes in basin flow dynamics. This study assessed how the combination of climate change and human management of river flow impacts metabolism of the Ebro River (the largest river basin in Spain, 86,100km(2)), assessed as gross primary production-GPP-and ecosystem respiration-ER. In order to investigate the influence of global change on freshwater ecosystems, an analysis of trends and frequencies from 25 sampling sites of the Ebro river basin was conducted. For this purpose, we examined the effect of anthropogenic flow control on river metabolism with a Granger causality study; simultaneously, took into account the effects of climate change, a period of extraordinary drought (largest in past 140years). We identified periods of sudden flow changes resulting from both human management and global climate effects. From 1998 to 2012, the Ebro River basin was trending toward a more autotrophic condition indicated by P/R ratio. Particularly, the results show that floods that occurred after long periods of low flows had a dramatic impact on the respiration (i.e., mineralization) capacity of the river. This approach allowed for a detailed characterization of the relationships between river metabolism and drought impacts at the watershed level. These findings may allow for a better understanding of the ecological impacts provoked by flow management, thus contributing to maintain the health of freshwater communities and ecosystem services that rely on their integrity.

  18. The Potential Importance of Conservation, Restoration and Altered Management Practices for Water Quality in the Wabash River Watershed

    Science.gov (United States)

    Yang, G.; Best, E. P.; Goodwin, S.

    2013-12-01

    Non-point source (NPS) pollution is one of the leading causes of water quality impairment within the United States. Conservation, restoration and altered management (CRAM) practices may effectively reduce NPS pollutants to receiving water bodies and enhance local and regional ecosystem services. Barriers for the implementation of CRAM include uncertainties related to the extent to which nutrients are removed by CRAM at various spatial and temporal scales, longevity, optimal placement of CRAM within the landscape, and implementation / operation / maintenance costs. We conducted a study aimed at the identification of optimal placement of CRAM in watersheds that reduces N loading to an environmentally sustainable level, at an acceptable, known, cost. For this study, we used a recently developed screening-level modeling approach, WQM-TMDL-N, running in the ArcGIS environment, to estimate nitrogen loading under current land use conditions (NLCD 2006). This model was equipped with a new option to explore the performances of placement of various CRAM types and areas to reduce nitrogen loading to a State-accepted Total Maximum Daily Load (TMDL) standard, with related annual average TN concentration, and a multi-objective algorithm optimizing load and cost. CRAM practices explored for implementation in rural area included buffer strips, nutrient management practices, and wetland restoration. We initially applied this modeling approach to the Tippecanoe River (TR) watershed (8-digit HUC), a headwater of the Wabash River (WR) watershed, where CRAM implementation in rural and urban areas is being planned and implemented at various spatial scales. Consequences of future land use are explored using a 2050 land use/land cover map forecasted by the Land Transformation Model. The WR watershed, IN, drains two-thirds of the state's 92 counties and supports predominantly agricultural land use. Because the WR accounts for over 40% of the nutrient loads of the Ohio River and

  19. Suspended sediment in a high-Arctic river

    DEFF Research Database (Denmark)

    Ladegaard-Pedersen, Pernille; Sigsgaard, Charlotte; Kroon, Aart;

    2016-01-01

    Quantifying fluxes of water, sediment and dissolved compounds through Arctic rivers is important for linking the glacial, terrestrial and marine ecosystems and to quantify the impact of a warming climate. The quantification of fluxes is not trivial. This study uses a 8-years data set (2005......-1 and 61,000±16,000ty-1. Extreme events with high discharges had a mean duration of 1day. The average suspended sediment flux during extreme events was 17,000±5000ty-1, which constitutes a year-to-year variation of 20-37% of the total annual flux. The most accurate sampling strategy was bi......-agreements and accuracies variations in the measured suspended sediment concentrations. The discharge weighted mean...

  20. The Water Quality of the River Enborne, UK: Observations from High-Frequency Monitoring in a Rural, Lowland River System

    Directory of Open Access Journals (Sweden)

    Sarah J. Halliday

    2014-01-01

    Full Text Available This paper reports the results of a 2-year study of water quality in the River Enborne, a rural river in lowland England. Concentrations of nitrogen and phosphorus species and other chemical determinands were monitored both at high-frequency (hourly, using automated in situ instrumentation, and by manual weekly sampling and laboratory analysis. The catchment land use is largely agricultural, with a population density of 123 persons km−2. The river water is largely derived from calcareous groundwater, and there are high nitrogen and phosphorus concentrations. Agricultural fertiliser is the dominant source of annual loads of both nitrogen and phosphorus. However, the data show that sewage effluent discharges have a disproportionate effect on the river nitrogen and phosphorus dynamics. At least 38% of the catchment population use septic tank systems, but the effects are hard to quantify as only 6% are officially registered, and the characteristics of the others are unknown. Only 4% of the phosphorus input and 9% of the nitrogen input is exported from the catchment by the river, highlighting the importance of catchment process understanding in predicting nutrient concentrations. High-frequency monitoring will be a key to developing this vital process understanding.

  1. VITRIFICATION OF HIGH LEVEL WASTE AT THE SAVANNAH RIVER SITE

    Energy Technology Data Exchange (ETDEWEB)

    Fox, K.; Peeler, D.

    2009-06-17

    The objective of this study was to experimentally measure the properties and performance of a series of glasses with compositions that could represent high level waste Sludge Batch 5 (SB5) as vitrified at the Savannah River Site Defense Waste Processing Facility. These data were used to guide frit optimization efforts as the SB5 composition was finalized. Glass compositions for this study were developed by combining a series of SB5 composition projections with a group of candidate frits. The study glasses were fabricated using depleted uranium and their chemical compositions, crystalline contents and chemical durabilities were characterized. Trevorite was the only crystalline phase that was identified in a few of the study glasses after slow cooling, and is not of concern as spinels have been shown to have little impact on the durability of high level waste glasses. Chemical durability was quantified using the Product Consistency Test (PCT). All of the glasses had very acceptable durability performance. The results of this study indicate that a frit composition can be identified that will provide a processable and durable glass when combined with SB5.

  2. High prevalence of multiple-antibiotic-resistant (MAR) Escherichia coli in river bed sediments of the Apies River, South Africa.

    Science.gov (United States)

    Abia, Akebe Luther King; Ubomba-Jaswa, Eunice; Momba, Maggy Ndombo Benteke

    2015-10-01

    This study aimed at investigating the presence of antibiotic-resistant Escherichia coli in river bed sediments of the Apies River, Gauteng, South Africa, in order to better inform health management decisions designed to protect users of the river. Overall, 180 water and sediment samples were collected at 10 sites along the Apies River from January to February 2014. E. coli was enumerated using the Colilert® 18/Quanti-Tray® 2000 (IDEXX). Isolates were purified by streaking on eosin methylene blue agar followed by the indole test. Pure E. coli isolates were tested for resistance to nine antibiotics by the Kirby-Bauer disc diffusion method. Over 98% of the isolates were resistant to at least one of the antibiotics tested. The highest resistance was observed against nitrofurantoin (sediments) and ampicillin (water). Over 80% of all resistant isolates showed multiple antibiotic resistance (resistance to ≥3 antibiotics). The abundance of E. coli in the sediments not only adds to the evidence that sediments are a reservoir for bacteria and possibly other pathogens including antibiotic-resistant bacteria but also suggests that antibiotic-resistant genes could be transferred to pathogens due to the high prevalence of multiple-antibiotic-resistant (MAR) strains of E. coli observed in the sediment. Using untreated water from the Apies River following resuspension for drinking and other household purposes could pose serious health risks for users. Our results suggest that river bed sediments could serve as reservoirs for MAR bacteria including pathogens under different climatic conditions and their analysis could provide information of public health concerns.

  3. Spermatogenic alterations in men with high testiculo epididymal temperatures

    Directory of Open Access Journals (Sweden)

    Dada Rima

    2002-01-01

    Full Text Available Sperms are produced by a highly complex and poorly understood differentiation process known as spermatogenesis. Occupational exposure to high temperatures adversely affect testicular function causing partial or complete spermatogenic arrest. Dyers, cooks, blast furnace workers and men with varicocele are known to develop testicular hyperthermia, which leads to oligoasthenoteratozoospermia (OAT and azoospermia. Semen analysis of 125 infertile men (and 25 fertile controls following the WHO guidelines, 1999 showed azoospermia in 109 men and oligozoospermia in 16 men. Twenty azoospermic and 14 oligozoospermic men had high testiculoepididymal temperatures either due to occupational exposure to high temperature or varicocele. All the 14 oligozoospermic men showed a very high percentage of sperms with abnormal morphology, impaired motility and they were subclassified as OAT group. Observations made in this study reiterates that high intratesticular temperature causes partial or complete spermatogenic arrest and may lead to increased production of morphologically abnormal sperms with impaired motility. This inverse relationship of sperm function with elevated temperature has implications in clinical medicine both in understanding pathological states and for therapeutic measures.

  4. The effects of water diversion and climate change on hydrological alteration and temperature regime of karst rivers in central Croatia.

    Science.gov (United States)

    Zganec, Krešimir

    2012-09-01

    Karst rivers in Croatia have been heavily dammed in the past. However, long-term impacts of dams, water diversion and climate change on karst rivers in Croatia have rarely been examined. This study analyzes long-term trends of hydrological and temperature conditions, prior to closure of new large dam, in five connected karst rivers of central Croatia: the sinking rivers Gornja Dobra and Zagorska Mrežnica that have underground connections with the Gojačka Dobra, Tounjčica and Mrežnica Rivers. It was established that hydrological conditions of these rivers have been greatly changed after the Gojak HPP, located at spring of Gojačka Dobra, became operational in 1959. Water diversion from the reservoirs on Zagorska Mrežnica and Gornja Dobra to the Gojak HPP has increased mean annual discharge (52-106%), causing great subdaily fluctuations and overall modification of natural flow regime along the whole course of Gojačka Dobra. By cutting the connection between Zagorska Mrežnica and Tounjčica, water diversion has caused a reduction of mean annual discharges of the Tounjčica (-60%) and Mrežnica (-29%) Rivers. Analyses of long-term trends in water temperature over the last 50 years have revealed significant positive trends in average annual temperature at three gauging stations on three rivers with increase of 0.17, 0.44 and 0.48°C per decade and climate change as the primary cause. The highest significant increasing trend was established at the lower course of Mrežnica where discharge was reduced by water diversion. Mitigation measures for the improvement of hydrological conditions of affected rivers and underground systems are proposed and discussed.

  5. Water quality assessment of highly polluted rivers in a semi-arid Mediterranean zone Oued Fez and Sebou River (Morocco)

    OpenAIRE

    2014-01-01

    Oued Fez (one of the Sebou River tributaries - Morocco) allowed us to study and quantify the effect of the lack of wastewater treatment on surface water quality in semi-arid hydrological context. The analysis is based on field data collected from June 2009 to December 2011. Concentration and load patterns of nitrogen, phosphorus and chromium (used in the processing of leather) are compared in stable hydrological conditions during low flow and high flow periods in an eight-location sampling ne...

  6. River

    Directory of Open Access Journals (Sweden)

    Morel Mathieu

    2016-01-01

    Full Text Available The OECD report “Boosting Resilience through Innovative Risk Governance” examines the efforts of OECD countries to prevent or reduce future disaster impacts, and highlights several key areas where improvements can be made. International collaboration is insufficiently utilised to address shocks that have increasingly global consequences. Institutional design plays a significant role in facilitating or hampering the engagement and investments of governmental and non-governmental stakeholders in disaster risk prevention and mitigation. To inform the design of “better” institutions, the OECD proposes the application of a diagnostic framework that helps governments identify institutional shortcomings and take actions to improve them. The goal of the case study on the Rhone River is to conduct an analysis of the progress, achievements and existing challenges in designing and implementing disaster risk reduction strategies through the Rhone Plan from a comparative perspective across a set of selected countries of this study, like Austria and Switzerland, will inform how to improve institutional frameworks governing risk prevention and mitigation. The case study will be used to identify examples of successful practice taking into account their specific country contexts, and analyse their potential for policy transfer.

  7. Effects of Human Alterations on Global River Basins and their Associated Coastal Zones: focus on River-dominated Ocean Margins (RiOMars)

    Science.gov (United States)

    Dürr, H. H.; Van Cappellen, P.; Meybeck, M.; Laruelle, G. G.; Mayorga, E.; Hartmann, J.; Maavara, T.; Bouwman, L.; Seitzinger, S.

    2013-12-01

    Coastal systems connected to large rivers, mostly major delta systems or river-dominated ocean margins (RiOMars), make up channels, creating a braided network of streams and many islands (Dürr et al. 2011). Here, we discuss controls on the different nutrient forms delivered to different coastal environments, and how they are assessed (Global-NEWS and other approaches), with a special focus on large deltas and RiOMars. Drivers and impacts of global change will be explored through the Millenium Assessment Scenarios and how the fluxes to these different coastal systems might change. An increasing role is also played by aquaculture in different coastal types as a non-insignificant source of nutrients. World-wide distribution of coastal types and their related river basins (Dürr et al. 2011). Characteristics of types of near-shore coastal areas and their associated river basins Greenland and Antarctica excepted. Data from Dürr et al. (2011) and the Global-NEWS program (Seitzinger et al. 2005 and Mayorga et al. 2010).

  8. The relationship betwwen ENSO cycle and high and low—flow in the upper Yellow River

    Institute of Scientific and Technical Information of China (English)

    LANYongchao; DINGYongjiang; KANGErsi; MAQuanjie; ZHANGJishi

    2003-01-01

    Firstly,the hydrological and meteorological features of the upper reaches of the Yellow River above Tangnag are analyzed based on observation data,and effects of El Nino and La Nina events on the high and low flow in the upper Yellow River are discussed.The results show El Nino and La Nina events possess consanguineous relationship with runoff in the upper Yellow River.As a whole,the probability of low flow occurrence in the upper Yellow River is relatively great along with the occurrence of El Nino event.Moreover,the flood in the upper Yellow River occurs frequently with the occurrence of La Nina event.Besides,the results also show dissimilarity of El Nino event occurring time exerts greater impact on high flow and low flow in the upper Yellow River,that is,the probability of drought will be greater in the same year if El Nino event occurs in spring,the high-flow may happen in this year if El Nino occurs in summer or autumn;the longer the continuous period of El Nino is,the lower the runoff in the upper Yellow River is.

  9. Detecting Human Hydrologic Alteration from Diversion Hydropower Requires Universal Flow Prediction Tools: A Proposed Framework for Flow Prediction in Poorly-gauged, Regulated Rivers

    Science.gov (United States)

    Kibler, K. M.; Alipour, M.

    2016-12-01

    Achieving the universal energy access Sustainable Development Goal will require great investment in renewable energy infrastructure in the developing world. Much growth in the renewable sector will come from new hydropower projects, including small and diversion hydropower in remote and mountainous regions. Yet, human impacts to hydrological systems from diversion hydropower are poorly described. Diversion hydropower is often implemented in ungauged rivers, thus detection of impact requires flow analysis tools suited to prediction in poorly-gauged and human-altered catchments. We conduct a comprehensive analysis of hydrologic alteration in 32 rivers developed with diversion hydropower in southwestern China. As flow data are sparse, we devise an approach for estimating streamflow during pre- and post-development periods, drawing upon a decade of research into prediction in ungauged basins. We apply a rainfall-runoff model, parameterized and forced exclusively with global-scale data, in hydrologically-similar gauged and ungauged catchments. Uncertain "soft" data are incorporated through fuzzy numbers and confidence-based weighting, and a multi-criteria objective function is applied to evaluate model performance. Testing indicates that the proposed framework returns superior performance (NSE = 0.77) as compared to models parameterized by rote calibration (NSE = 0.62). Confident that the models are providing `the right answer for the right reasons', our analysis of hydrologic alteration based on simulated flows indicates statistically significant hydrologic effects of diversion hydropower across many rivers. Mean annual flows, 7-day minimum and 7-day maximum flows decreased. Frequency and duration of flow exceeding Q25 decreased while duration of flows sustained below the Q75 increased substantially. Hydrograph rise and fall rates and flow constancy increased. The proposed methodology may be applied to improve diversion hydropower design in data-limited regions.

  10. Hidden Markov models for the assessment of chromosomal alterations using high-throughput SNP arrays

    OpenAIRE

    2008-01-01

    Chromosomal DNA is characterized by variation between individuals at the level of entire chromosomes (e.g., aneuploidy in which the chromosome copy number is altered), segmental changes (including insertions, deletions, inversions, and translocations), and changes to small genomic regions (including single nucleotide polymorphisms). A variety of alterations that occur in chromosomal DNA, many of which can be detected using high density single nucleotide polymorphism (SNP)...

  11. High Performance Computing-based Assessment of the Impacts of Climate Change on the Santa Cruz and San Pedro River Basin at Very High Resolution

    Science.gov (United States)

    Robles-Morua, A.; Vivoni, E. R.; Rivera-Fernandez, E. R.; Dominguez, F.; Meixner, T.

    2012-12-01

    Assessing the impact of climate change on large river basins in the southwestern United States is important given the natural water scarcity in the region. The bimodal distribution of annual precipitation also presents a challenge as differential climate impacts during the winter and summer seasons are not currently well understood. In this work, we focus on the hydrological consequences of climate change in the Santa Cruz and San Pedro river basins along the Arizona-Sonora border at high spatiotemporal resolutions (~100 m and ~1 hour). These river systems support rich ecological communities along riparian corridors that provide habitat to migratory birds and support recreational and economic activities. Determining the climate impacts on riparian communities involves assessing how river flows and groundwater recharge will change with altered temperature and precipitation regimes. In this study, we use a distributed hydrologic model, known as the TIN-based Real-time Integrated Basin Simulator (tRIBS), to generate simulated hydrological fields under historical (1991-2000) and climate change (2031-2040) scenarios obtained from an application of the Weather Research and Forecast (WRF) model. Using the distributed model, we transform the meteorological scenarios from WRF at 10-km, hourly resolution into predictions of the annual water budget, seasonal land surface fluxes and individual hydrographs of flood and recharge events. For this contribution, we selected two full years in the historical period and in the future scenario that represent wet and dry conditions for each decade. Given the size of the two basins, we rely on a high performance computing platform and a parallel domain discretization using sub-basin partitioning with higher resolutions maintained at experimental catchments in each river basin. Model simulations utilize best-available data across the Arizona-Sonora border on topography, land cover and soils obtained from analysis of remotely

  12. Automated Identification of River Hydromorphological Features Using UAV High Resolution Aerial Imagery.

    Science.gov (United States)

    Casado, Monica Rivas; Gonzalez, Rocio Ballesteros; Kriechbaumer, Thomas; Veal, Amanda

    2015-11-04

    European legislation is driving the development of methods for river ecosystem protection in light of concerns over water quality and ecology. Key to their success is the accurate and rapid characterisation of physical features (i.e., hydromorphology) along the river. Image pattern recognition techniques have been successfully used for this purpose. The reliability of the methodology depends on both the quality of the aerial imagery and the pattern recognition technique used. Recent studies have proved the potential of Unmanned Aerial Vehicles (UAVs) to increase the quality of the imagery by capturing high resolution photography. Similarly, Artificial Neural Networks (ANN) have been shown to be a high precision tool for automated recognition of environmental patterns. This paper presents a UAV based framework for the identification of hydromorphological features from high resolution RGB aerial imagery using a novel classification technique based on ANNs. The framework is developed for a 1.4 km river reach along the river Dee in Wales, United Kingdom. For this purpose, a Falcon 8 octocopter was used to gather 2.5 cm resolution imagery. The results show that the accuracy of the framework is above 81%, performing particularly well at recognising vegetation. These results leverage the use of UAVs for environmental policy implementation and demonstrate the potential of ANNs and RGB imagery for high precision river monitoring and river management.

  13. Automated Identification of River Hydromorphological Features Using UAV High Resolution Aerial Imagery

    Directory of Open Access Journals (Sweden)

    Monica Rivas Casado

    2015-11-01

    Full Text Available European legislation is driving the development of methods for river ecosystem protection in light of concerns over water quality and ecology. Key to their success is the accurate and rapid characterisation of physical features (i.e., hydromorphology along the river. Image pattern recognition techniques have been successfully used for this purpose. The reliability of the methodology depends on both the quality of the aerial imagery and the pattern recognition technique used. Recent studies have proved the potential of Unmanned Aerial Vehicles (UAVs to increase the quality of the imagery by capturing high resolution photography. Similarly, Artificial Neural Networks (ANN have been shown to be a high precision tool for automated recognition of environmental patterns. This paper presents a UAV based framework for the identification of hydromorphological features from high resolution RGB aerial imagery using a novel classification technique based on ANNs. The framework is developed for a 1.4 km river reach along the river Dee in Wales, United Kingdom. For this purpose, a Falcon 8 octocopter was used to gather 2.5 cm resolution imagery. The results show that the accuracy of the framework is above 81%, performing particularly well at recognising vegetation. These results leverage the use of UAVs for environmental policy implementation and demonstrate the potential of ANNs and RGB imagery for high precision river monitoring and river management.

  14. Alteration Map Showing Major Faults and Veins and Associated Water-Quality Signatures of the Animas River Watershed Headwaters Near Silverton, Southwest Colorado

    Science.gov (United States)

    Bove, Dana J.; Yager, Douglas B.; Mast, M. Alisa; Dalton, J. Brad

    2007-01-01

    This map was produced to provide hard-copy and digital data for alteration assemblages in the historical mining area centered on the Tertiary San Juan and Silverton calderas. The data have direct application to geoenvironmental and mineral exploration objectives. This dataset represents alteration mapping for the upper Animas River watershed near Silverton, Colorado. The map is based on detailed 1:12,000-scale field mapping, X-ray diffraction (XRD) analysis, mineral mapping by remote sensing (AVIRIS) data, and 1:24,000-scale aerial photographic interpretation. Geologic structures were compiled and generalized from multiple published and unpublished sources (Burbank and Luedke, 1964; Steven and others, 1974; Luedke and Burbank 1975a, b; Lipman, 1976; Luedke and Burbank, 1987; Luedke, 1996) (see Index Map). Unpublished mapping of the Ironton quadrangle by D.J. Bove and J.P. Kurtz in 1997-1999 was included.

  15. Is inversion based high resolution characterization of spatially heterogeneous river bed hydraulic conductivity needed and possible?

    Directory of Open Access Journals (Sweden)

    W. Kurtz

    2013-05-01

    Full Text Available River-aquifer exchange fluxes influence local and regional water balances and affect groundwater and river water quality and quantity. Unfortunately, river-aquifer exchange fluxes tend to be strongly spatially variable and it is an open research question to which degree river bed heterogeneity has to be represented in a~model in order to achieve reliable estimates of river-aquifer exchange fluxes. This research question is addressed in this paper with help of synthetic simulation experiments, which mimic the Limmat aquifer in Zurich (Switzerland, where river-aquifer exchange fluxes and groundwater management activities play an important role. The solution of the unsaturated-saturated subsurface hydrological flow problem including river-aquifer interaction is calculated for ten different synthetic realities where the strongly heterogeneous river bed hydraulic conductivities (L are perfectly known. Hydraulic head data (100 in the default scenario are sampled from the synthetic realities. In subsequent data assimilation experiments, where L is unknown now, the hydraulic head data are used as conditioning information, with help of the Ensemble Kalman Filter (EnKF. For each of the ten synthetic realities, four different ensembles of L are tested in the experiments with EnKF; one ensemble estimates high resolution L-fields with different L values for each element, and the other three ensembles estimate effective L values for 5, 3 or 2 zones. The calibration of higher resolution L-fields (i.e., fully heterogeneous or 5 zones gives better results than the calibration of L for only 3 or 2 zones in terms of reproduction of states, stream-aquifer exchange fluxes and parameters. Effective L for a limited number of zones cannot always reproduce the true states and fluxes well and results in biased estimates of net exchange fluxes between aquifer and stream. Also in case only 10 head data are used for conditioning, the high resolution L-fields outperform the

  16. Maintaining population persistence in the face of an extremely altered hydrograph: implications for three sensitive fishes in a tributary of the Green River, Utah

    Science.gov (United States)

    Bottcher, Jared L.

    2009-01-01

    The ability of an organism to disperse to suitable habitats, especially in modified and fragmented systems, determines individual fitness and overall population viability. The bluehead sucker (Catostomus discobolus), flannelmouth sucker (Catostomus latipinnis), and roundtail chub (Gila robusta) are three species native to the upper Colorado River Basin that now occupy only 50% of their historic range. Despite these distributional declines, populations of all three species are present in the San Rafael River, a highly regulated tributary of the Green River, Utah, providing an opportunity for research. Our goal was to determine the timing and extent of movement, habitat preferences, and limiting factors, ultimately to guide effective management and recovery of these three species. In 2007-2008, we sampled fish from 25 systematically selected, 300-m reaches in the lower 64 km of the San Rafael River, spaced to capture the range of species, life-stages, and habitat conditions present. We implanted all target species with a passive integrated transponder (PIT) tag, installed a passive PIT tag antennae, and measured key habitat parameters throughout each reach and at the site of native fish capture. We used random forest modeling to identify and rank the most important abiotic and biotic predictor variables, and reveal potential limiting factors in the San Rafael River. While flannelmouth sucker were relatively evenly distributed within our study area, highest densities of roundtail chub and bluehead sucker occurred in isolated, upstream reaches characterized by complex habitat. In addition, our movement and length-frequency data indicate downstream drift of age-0 roundtail chub, and active upstream movement of adult flannelmouth sucker, both from source populations, providing the lower San Rafael River with colonists. Our random forest analysis highlights the importance of pools, riffles, and distance-to-source populations, suggesting that bluehead sucker and roundtail

  17. Histopathological alterations observed in the liver of Poecilia vivipara (Cyprinodontiformes: Poeciliidae as a tool for the environmental quality assessment of the Cachoeira River, BA

    Directory of Open Access Journals (Sweden)

    DV Paulo

    Full Text Available Histopathological alterations in liver have been widely used as a tool in studies for monitoring environmental quality. To evaluate the environmental quality in the Cachoeira river, five spots were monitored between the municipal districts of Itapé and Ilhéus, using liver histological analysis. The species chosen for analysis was Poecilia vivipara due to the fact that it is one of the most abundant in the sampling. The routine technique of inclusion and impregnation in paraffin was used, and the cuts were stained with Hematoxylin and Eosin (H & E. Histopathological alterations in the liver were evaluated semi-quantitatively and based on the severity of the lesions. The results of the histopathological alteration frequency together with the average taken from the Histopathological Alteration Index from points 1 (Vila de Itapé and 2 (Fazenda Santa Amélia showed that in this area the environment is more threatened because of some stressor agent, possibly contaminants that seem to be acting in the environment and endangering the health of fish. The statistic results demonstrated that there were no significant differences among points 1, 2 and 4, which means they are very similar to one another, and are ecologically endangered.

  18. Reservoir response to thermal and high-pressure well stimulation efforts at Raft River, Idaho

    Energy Technology Data Exchange (ETDEWEB)

    Plummer, Mitchell [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bradford, Jacob [Energy & Geoscience Institute at the Univ. of Utah, Salt Lake City, UT (United States); Moore, Joseph [Energy & Geoscience Institute at the Univ. of Utah, Salt Lake City, UT (United States); Podgorney, Robert [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-08-01

    An injection stimulation test begun at the Raft River geothermal reservoir in June, 2013 has produced a wealth of data describing well and reservoir response via high-resolution temperature logging and distributed temperature sensing, seismic monitoring, periodic borehole televiewer logging, periodic stepped flow rate tests and tracer injections before and after stimulation efforts. One of the primary measures of response to the stimulation is the relationship between fluid pressure and flow rate, short-term during forced flow rate changes and the long-term change in injectivity. In this paper we examine that hydraulic response using standard pumping test analysis methods, largely because pressure response to the stimulation was not detected, or measurable, in other wells. Analysis of stepped rate flow tests supports the inference from other data that a large fracture, with a radial extent of one to several meters, intersects the well in the target reservoir, suggests that the flow regime is radial to a distance of only several meters and demonstrates that the pressure build-up cone reaches an effective constant head at that distance. The well’s longer term hydraulic response demonstrated continually increasing injectivity but at a dramatically faster rate later from ~2 years out and continuing to the present. The net change in injectivity is significantly greater than observed in other longterm injectivity monitoring studies, with an approximately 150–fold increase occurring over ~2.5 years. While gradually increasing injectivity is a likely consequence of slow migration of a cooling front, and consequent dilation of fractures, the steady, ongoing, rate of increase is contrary to what would be expected in a radial or linear flow regime, where the cooling front would slow with time. As a result, occasional step-like changes in injectivity, immediately following high-flow rate tests suggest that hydro shearing during high-pressure testing altered the near

  19. Altered multiaxial mechanical properties of the porcine anterior lens capsule cultured in high glucose.

    Science.gov (United States)

    Pedrigi, R M; Staff, E; David, G; Glenn, S; Humphrey, J D

    2007-02-01

    Hyperglycemia can alter the mechanical properties of tissues through the formation of advanced glycation endproducts in matrix proteins that have long half-lives. We used a custom experimental system and subdomain finite element method to quantify alterations in the regional multiaxial mechanical properties of porcine lens capsules that were cultured for 8 or 14 weeks in high glucose versus control media. Findings revealed that high glucose significantly stiffened the capsules in both the circumferential and the meridional directions, but it did not affect the known regional variations in anisotropy. Such information could be important in the design of both improved clinical procedures and intraocular implants for diabetic patients.

  20. Sedimentary BSi and TOC quantifies the degradation of the Changjiang Estuary, China, from river basin alteration and warming SST

    Science.gov (United States)

    Li, Maotian; Wang, Hong; Li, Yimiao; Ai, Wei; Hou, Lijun; Chen, Zhongyuan

    2016-12-01

    Environmental degradation in the Changjiang Estuary has recently become a global topic, given its proximity to Shanghai with a population >23 million. Intensification of human activities affecting the river basin is responsible for this degradation. Dam construction has cut off ca. 2/3 of the sediment flux to the sea, ca. 60% of the dissolved silicate load (DSi) has been retained in the reservoirs, while total phosphorous (TP) and total nitrogen (TN) transport to the sea are many times more than they were a few decades ago. Under such circumstances, details of the estuarine degradation remain poorly understood. This study uses sedimentary biological silicate (BSi) and total organic carbon (TOC) as environmental proxies to reveal the process-response of such degradation since the 1950s. Our results demonstrate the spatial differences of such degradation. The inner zone of the estuary used to be highly turbid, but presently has increasing diatom (BSi) and primary production (TOC), due to lower suspended sediment concentration (SSC) in relation to dam construction. In contrast, increasing riverine dissolved inorganic nitrate (DIN) and dissolved inorganic phosphorous (DIP) input (up to 2-5 times) and decreasing DSi provide a unique setting, with an excess in N and P, which catalyzes non-diatom algae in the less-turbid middle zone of the estuary. These are reflected by decreasing BSi and BSi/TOC since the 1950s, together with an increase of TOC of 20-40%. In the outer zone of the estuary, increasing DIN, DIP, and sea surface temperatures (SSTs), have resulted in the increase of diatom biomass by 15-20% and the growth of primary production by 30-60% since the 1950s. But the drastic decrease in DSi, Si/N, and Si/P depresses the ability of diatoms to develop, resulting in a reduction of 5-10% diatom proportion (BSi/TOC) since the 1930s. This study improves the understanding of the changing estuarine ecosystem in response to global change.

  1. Demonstration of Caustic-Side Solvent Extraction with Savannah River Site High Level Waste

    Energy Technology Data Exchange (ETDEWEB)

    Walker, D.D.

    2001-08-27

    Researchers successfully demonstrated the chemistry and process equipment of the Caustic-Side Solvent Extraction (CSSX) flowsheet for the decontamination of high level waste using a 33-stage, 2-cm centrifugal contactor apparatus at the Savannah River Technology Center. This represents the first CSSX process demonstration using Savannah River Site (SRS) high level waste. Three tests lasting 6, 12, and 48 hours processed simulated average SRS waste, simulated Tank 37H/44F composite waste, and Tank 37H/44F high level waste, respectively.

  2. Golden alga presence and abundance are inversely related to salinity in a high-salinity river ecosystem, Pecos River, USA

    Science.gov (United States)

    Israël, Natascha M.D.; VanLandeghem, Matthew M.; Denny, Shawn; Ingle, John; Patino, Reynaldo

    2014-01-01

    Prymnesium parvum (golden alga, GA) is a toxigenic harmful alga native to marine ecosystems that has also affected brackish inland waters. The first toxic bloom of GA in the western hemisphere occurred in the Pecos River, one of the saltiest rivers in North America. Environmental factors (water quality) associated with GA occurrence in this basin, however, have not been examined. Water quality and GA presence and abundance were determined at eight sites in the Pecos River basin with or without prior history of toxic blooms. Sampling was conducted monthly from January 2012 to July 2013. Specific conductance (salinity) varied spatiotemporally between 4408 and 73,786 mS/cm. Results of graphical, principal component (PCA), and zero-inflated Poisson (ZIP) regression analyses indicated that the incidence and abundance of GA are reduced as salinity increases spatiotemporally. LOWESS regression and correlation analyses of archived data for specific conductance and GA abundance at one of the study sites retrospectively confirmed the negative association between these variables. Results of PCA also suggested that at nutrient (nitrogen and phosphorus) concentrations whereas at higher salinity, GA was observed only at mid-to-high nutrient levels. Generally consistent with earlier studies, results of ZIP regression indicated that GA presence is positively associated with organic phosphorus and in samples where GA is present, GA abundance is positively associated with organic nitrogen and negatively associated with inorganic nitrogen. This is the first report of an inverse relation between salinity and GA presence and abundance in riverine waters and of interaction effects of salinity and nutrients in the field. These observations contribute to a more complete understanding of environmental conditions that influence GA distribution in inland waters.

  3. Hydrochemical evidence for mixing of river water and groundwater during high-flow conditions, lower Suwannee River basin, Florida, USA

    Science.gov (United States)

    Crandall, Christy A.; Katz, Brian G.; Hirten, Joshua J.

    Karstic aquifers are highly susceptible to rapid infiltration of river water, particularly during periods of high flow. Following a period of sustained rainfall in the Suwannee River basin, Florida, USA, the stage of the Suwannee River rose from 3.0 to 5.88m above mean sea level in April 1996 and discharge peaked at 360m3/s. During these high-flow conditions, water from the Suwannee River migrated directly into the karstic Upper Floridan aquifer, the main source of water supply for the area. Changes in the chemical composition of groundwater were quantified using naturally occurring geochemical tracers and mass-balance modeling techniques. Mixing of river water with groundwater was indicated by a decrease in the concentrations of calcium, silica, and 222Rn; and by an increase in dissolved organic carbon (DOC), tannic acid, and chloride, compared to low-flow conditions in water from a nearby monitoring well, Wingate Sink, and Little River Springs. The proportion (fraction) of river water in groundwater ranged from 0.13 to 0.65 at Wingate Sink and from 0.5 to 0.99 at well W-17258, based on binary mixing models using various tracers. The effectiveness of a natural tracer in quantifying mixing of river water and groundwater was related to differences in tracer concentration of the two end members and how conservatively the tracer reacted in the mixed water. Solutes with similar concentrations in the two end-member waters (Na, Mg, K, Cl, SO4, SiO2) were not as effective tracers for quantifying mixing of river water and groundwater as those with larger differences in end-member concentrations (Ca, tannic acid, DOC, 222Rn, HCO3). Résumé Les aquifères karstiques sont particulièrement sensibles à l'infiltration rapide d'eau de rivières, spécialement pendant les périodes de hautes eaux. A la suite d'une période de pluies soutenues sur le bassin de la rivière Suwannee (Floride, États-Unis), le niveau de cette rivière est monté de 3,0 à 5,88m au-dessus du niveau

  4. Fish mediate high food web connectivity in the lower reaches of a tropical floodplain river.

    Science.gov (United States)

    Jardine, Timothy D; Pusey, Bradley J; Hamilton, Stephen K; Pettit, Neil E; Davies, Peter M; Douglas, Michael M; Sinnamon, Vivian; Halliday, Ian A; Bunn, Stuart E

    2012-03-01

    High levels of hydrological connectivity during seasonal flooding provide significant opportunities for movements of fish between rivers and their floodplains, estuaries and the sea, possibly mediating food web subsidies among habitats. To determine the degree of utilisation of food sources from different habitats in a tropical river with a short floodplain inundation duration (~2 months), stable isotope ratios in fishes and their available food were measured from three habitats (inundated floodplain, dry season freshwater, coastal marine) in the lower reaches of the Mitchell River, Queensland (Australia). Floodplain food sources constituted the majority of the diet of large-bodied fishes (barramundi Lates calcarifer, catfish Neoarius graeffei) captured on the floodplain in the wet season and for gonadal tissues of a common herbivorous fish (gizzard shad Nematalosa come), the latter suggesting that critical reproductive phases are fuelled by floodplain production. Floodplain food sources also subsidised barramundi from the recreational fishery in adjacent coastal and estuarine areas, and the broader fish community from a freshwater lagoon. These findings highlight the importance of the floodplain in supporting the production of large fishes in spite of the episodic nature and relatively short duration of inundation compared to large river floodplains of humid tropical regions. They also illustrate the high degree of food web connectivity mediated by mobile fish in this system in the absence of human modification, and point to the potential consequences of water resource development that may reduce or eliminate hydrological connectivity between the river and its floodplain.

  5. Normalization of High Dimensional Genomics Data Where the Distribution of the Altered Variables Is Skewed

    Science.gov (United States)

    Landfors, Mattias; Philip, Philge; Rydén, Patrik; Stenberg, Per

    2011-01-01

    Genome-wide analysis of gene expression or protein binding patterns using different array or sequencing based technologies is now routinely performed to compare different populations, such as treatment and reference groups. It is often necessary to normalize the data obtained to remove technical variation introduced in the course of conducting experimental work, but standard normalization techniques are not capable of eliminating technical bias in cases where the distribution of the truly altered variables is skewed, i.e. when a large fraction of the variables are either positively or negatively affected by the treatment. However, several experiments are likely to generate such skewed distributions, including ChIP-chip experiments for the study of chromatin, gene expression experiments for the study of apoptosis, and SNP-studies of copy number variation in normal and tumour tissues. A preliminary study using spike-in array data established that the capacity of an experiment to identify altered variables and generate unbiased estimates of the fold change decreases as the fraction of altered variables and the skewness increases. We propose the following work-flow for analyzing high-dimensional experiments with regions of altered variables: (1) Pre-process raw data using one of the standard normalization techniques. (2) Investigate if the distribution of the altered variables is skewed. (3) If the distribution is not believed to be skewed, no additional normalization is needed. Otherwise, re-normalize the data using a novel HMM-assisted normalization procedure. (4) Perform downstream analysis. Here, ChIP-chip data and simulated data were used to evaluate the performance of the work-flow. It was found that skewed distributions can be detected by using the novel DSE-test (Detection of Skewed Experiments). Furthermore, applying the HMM-assisted normalization to experiments where the distribution of the truly altered variables is skewed results in considerably higher

  6. Normalization of high dimensional genomics data where the distribution of the altered variables is skewed.

    Directory of Open Access Journals (Sweden)

    Mattias Landfors

    Full Text Available Genome-wide analysis of gene expression or protein binding patterns using different array or sequencing based technologies is now routinely performed to compare different populations, such as treatment and reference groups. It is often necessary to normalize the data obtained to remove technical variation introduced in the course of conducting experimental work, but standard normalization techniques are not capable of eliminating technical bias in cases where the distribution of the truly altered variables is skewed, i.e. when a large fraction of the variables are either positively or negatively affected by the treatment. However, several experiments are likely to generate such skewed distributions, including ChIP-chip experiments for the study of chromatin, gene expression experiments for the study of apoptosis, and SNP-studies of copy number variation in normal and tumour tissues. A preliminary study using spike-in array data established that the capacity of an experiment to identify altered variables and generate unbiased estimates of the fold change decreases as the fraction of altered variables and the skewness increases. We propose the following work-flow for analyzing high-dimensional experiments with regions of altered variables: (1 Pre-process raw data using one of the standard normalization techniques. (2 Investigate if the distribution of the altered variables is skewed. (3 If the distribution is not believed to be skewed, no additional normalization is needed. Otherwise, re-normalize the data using a novel HMM-assisted normalization procedure. (4 Perform downstream analysis. Here, ChIP-chip data and simulated data were used to evaluate the performance of the work-flow. It was found that skewed distributions can be detected by using the novel DSE-test (Detection of Skewed Experiments. Furthermore, applying the HMM-assisted normalization to experiments where the distribution of the truly altered variables is skewed results in

  7. Non–invasive sampling of endangered neotropical river otters reveals high levels of dispersion in the Lacantun River System of Chiapas, Mexico

    Directory of Open Access Journals (Sweden)

    Ortega, J.

    2012-01-01

    Full Text Available Patterns of genetic dispersion, levels of population genetic structure, and movement of the neotropical river otter (Lontra longicaudis were investigated by screening eight polymorphic microsatellites from DNA extracted from fecal samples, collected in a hydrologic system of the Lacandon rainforest in Chiapas, Mexico. A total of 34 unique genotypes were detected from our surveys along six different rivers, and the effect of landscape genetic structure was studied. We recovered 16 of the 34 individuals in multiple rivers at multiple times. We found high levels of dispersion and low levels of genetic differentiation among otters from the six surveyed rivers (P > 0.05, except for the pairwise comparison among the Lacantún and José rivers (P < 0.05. We recommend that conservation management plans for the species consider the entire Lacantún River System and its tributaries as a single management unit to ensure the maintenance of current levels of population genetic diversity, because the population analyzed seems to follow a source–sink dynamic mainly determined by the existence of the major river.

  8. Oak Park and River Forest High School Random Access Information Center; A PACE Program. Report II.

    Science.gov (United States)

    Oak Park - River Forest High School, Oak Park, IL.

    The specifications, planning, and initial development phases of the Random Access Center at the Oak Park and River Forest High School in Oak Park, Illinois, are described with particular attention to the ways that the five functional specifications and the five-part program rationale were implemented in the system design. Specifications, set out…

  9. Response surfaces of vulnerability to climate change: The Colorado River Basin, the High Plains, and California

    Science.gov (United States)

    Romano Foti; Jorge A. Ramirez; Thomas C. Brown

    2014-01-01

    We quantify the vulnerability of water supply to shortage for the Colorado River Basin and basins of the High Plains and California and assess the sensitivity of their water supply system to future changes in the statistical variability of supply and demand. We do so for current conditions and future socio-economic scenarios within a probabilistic framework that...

  10. 75 FR 38768 - Ashley National Forest, UT, High Uintas Wilderness-Colorado River Cutthroat Trout Habitat...

    Science.gov (United States)

    2010-07-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Ashley National Forest, UT, High Uintas Wilderness--Colorado River Cutthroat Trout Habitat Enhancement AGENCY: Forest Service, USDA. ACTION: Notice of intent to prepare an environmental...

  11. Non-myogenic tumors display altered expression of dystrophin (DMD) and a high frequency of genetic alterations

    Science.gov (United States)

    Luce, Leonela N.; Abbate, Mercedes

    2017-01-01

    DMD gene mutations have been associated with the development of Dystrophinopathies. Interestingly, it has been recently reported that DMD is involved in the development and progression of myogenic tumors, assigning DMD a tumor suppressor activity in these types of cancer. However, there are only few reports that analyze DMD in non-myogenic tumors. Our study was designed to examine DMD expression and genetic alterations in non-myogenic tumors using public repositories. We also evaluated the overall survival of patients with and without DMD mutations. We studied 59 gene expression microarrays (GEO database) and RNAseq (cBioPortal) datasets that included 9817 human samples. We found reduced DMD expression in 15/27 (56%) pairwise comparisons performed (Fold-Change (FC) ≤ 0.70; p-value range = 0.04-1.5×10−20). The analysis of RNAseq studies revealed a median frequency of DMD genetic alterations of 3.4%, higher or similar to other well-known tumor suppressor genes. In addition, we observed significant poorer overall survival for patients with DMD mutations. The analyses of paired tumor/normal tissues showed that the majority of tumor specimens had lower DMD expression compared to their normal adjacent counterpart. Interestingly, statistical significant over-expression of DMD was found in 6/27 studies (FC ≥ 1.4; p-value range = 0.03-3.4×10−15). These results support that DMD expression and genetic alterations are frequent and relevant in non-myogenic tumors. The study and validation of DMD as a new player in tumor development and as a new prognostic factor for tumor progression and survival are warranted. PMID:27391342

  12. Catchment-scale conservation units identified for the threatened Yarra pygmy perch (Nannoperca obscura in highly modified river systems.

    Directory of Open Access Journals (Sweden)

    Chris J Brauer

    Full Text Available Habitat fragmentation caused by human activities alters metapopulation dynamics and decreases biological connectivity through reduced migration and gene flow, leading to lowered levels of population genetic diversity and to local extinctions. The threatened Yarra pygmy perch, Nannoperca obscura, is a poor disperser found in small, isolated populations in wetlands and streams of southeastern Australia. Modifications to natural flow regimes in anthropogenically-impacted river systems have recently reduced the amount of habitat for this species and likely further limited its opportunity to disperse. We employed highly resolving microsatellite DNA markers to assess genetic variation, population structure and the spatial scale that dispersal takes place across the distribution of this freshwater fish and used this information to identify conservation units for management. The levels of genetic variation found for N. obscura are amongst the lowest reported for a fish species (mean heterozygosity of 0.318 and mean allelic richness of 1.92. We identified very strong population genetic structure, nil to little evidence of recent migration among demes and a minimum of 11 units for conservation management, hierarchically nested within four major genetic lineages. A combination of spatial analytical methods revealed hierarchical genetic structure corresponding with catchment boundaries and also demonstrated significant isolation by riverine distance. Our findings have implications for the national recovery plan of this species by demonstrating that N. obscura populations should be managed at a catchment level and highlighting the need to restore habitat and avoid further alteration of the natural hydrology.

  13. Radiation-induced epigenetic alterations after low and high LET irradiations

    Energy Technology Data Exchange (ETDEWEB)

    Aypar, Umut, E-mail: uaypa001@umaryland.edu [Department of Radiation Oncology, Radiation Oncology Research Laboratory, University of Maryland School of Medicine, Baltimore, MD 21201 (United States); Morgan, William F. [Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352 (United States); Baulch, Janet E. [Department of Radiation Oncology, Radiation Oncology Research Laboratory, University of Maryland School of Medicine, Baltimore, MD 21201 (United States)

    2011-02-10

    Epigenetics, including DNA methylation and microRNA (miRNA) expression, could be the missing link in understanding radiation-induced genomic instability (RIGI). This study tests the hypothesis that irradiation induces epigenetic aberrations, which could eventually lead to RIGI, and that the epigenetic aberrations induced by low linear energy transfer (LET) irradiation are different than those induced by high LET irradiations. GM10115 cells were irradiated with low LET X-rays and high LET iron (Fe) ions and evaluated for DNA damage, cell survival and chromosomal instability. The cells were also evaluated for specific locus methylation of nuclear factor-kappa B (NF{kappa}B), tumor suppressor in lung cancer 1 (TSLC1) and cadherin 1 (CDH1) gene promoter regions, long interspersed nuclear element 1 (LINE-1) and Alu repeat element methylation, CpG and non-CpG global methylation and miRNA expression levels. Irradiated cells showed increased micronucleus induction and cell killing immediately following exposure, but were chromosomally stable at delayed times post-irradiation. At this same delayed time, alterations in repeat element and global DNA methylation and miRNA expression were observed. Analyses of DNA methylation predominantly showed hypomethylation, however hypermethylation was also observed. We demonstrate that miRNA expression levels can be altered after X-ray irradiation and that these miRNA are involved in chromatin remodeling and DNA methylation. A higher incidence of epigenetic changes was observed after exposure to X-rays than Fe ions even though Fe ions elicited more chromosomal damage and cell killing. This distinction is apparent at miRNA analyses at which only three miRNA involved in two major pathways were altered after high LET irradiations while six miRNA involved in five major pathways were altered after low LET irradiations. This study also shows that the irradiated cells acquire epigenetic changes suggesting that epigenetic aberrations may arise

  14. Restoring ecological integrity in highly regulated rivers: the role of baseline data and analytical references.

    Science.gov (United States)

    Downs, Peter W; Singer, Maia S; Orr, Bruce K; Diggory, Zooey E; Church, Tamara C; Stella, J C

    2011-10-01

    The goal of restoring ecological integrity in rivers is frequently accompanied by an assumption that a comparative reference reach can be identified to represent minimally impaired conditions. However, in many regulated rivers, no credible historical, morphological or process-based reference reach exists. Resilient restoration designs should instead be framed around naturalization, using multiple analytical references derived from empirically-calibrated field- and model-based techniques to develop an integrated ecological reference condition. This requires baseline data which are rarely collected despite increasing evidence for systematic deficiencies in restoration practice. We illustrate the utility of baseline data collection in restoration planning for the highly fragmented and regulated lower Merced River, California, USA. The restoration design was developed using various baseline data surveys, monitoring, and modeling within an adaptive management framework. Baseline data assisted in transforming conceptual models of ecosystem function into specific restoration challenges, defining analytical references of the expected relationships among ecological parameters required for restoration, and specifying performance criteria for post-project monitoring and evaluation. In this way the study is an example of process-based morphological restoration designed to prompt recovery of ecosystem processes and resilience. For the Merced River, we illustrate that project-specific baseline data collection is a necessary precursor in developing performance-based restoration designs and addressing scale-related uncertainties, such as whether periodic gravel augmentation will sustain bed recovery and whether piecemeal efforts will improve ecological integrity. Given the numerous impediments to full, historical, restoration in many river systems, it seems apparent that projects of naturalization are a critical step in reducing the deleterious impacts of fragmented rivers

  15. High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress.

    Directory of Open Access Journals (Sweden)

    Eui Seok Shin

    Full Text Available Astrocytes are macroglial cells that have a crucial role in development of the retinal vasculature and maintenance of the blood-retina-barrier (BRB. Diabetes affects the physiology and function of retinal vascular cells including astrocytes (AC leading to breakdown of BRB. However, the detailed cellular mechanisms leading to retinal AC dysfunction under high glucose conditions remain unclear. Here we show that high glucose conditions did not induce the apoptosis of retinal AC, but instead increased their rate of DNA synthesis and adhesion to extracellular matrix proteins. These alterations were associated with changes in intracellular signaling pathways involved in cell survival, migration and proliferation. High glucose conditions also affected the expression of inflammatory cytokines in retinal AC, activated NF-κB, and prevented their network formation on Matrigel. In addition, we showed that the attenuation of retinal AC migration under high glucose conditions, and capillary morphogenesis of retinal endothelial cells on Matrigel, was mediated through increased oxidative stress. Antioxidant proteins including heme oxygenase-1 and peroxiredoxin-2 levels were also increased in retinal AC under high glucose conditions through nuclear localization of transcription factor nuclear factor-erythroid 2-related factor-2. Together our results demonstrated that high glucose conditions alter the function of retinal AC by increased production of inflammatory cytokines and oxidative stress with significant impact on their proliferation, adhesion, and migration.

  16. Late Paleocene high Laramide ranges in northeast Wyoming: Oxygen isotope study of ancient river water

    Science.gov (United States)

    Fan, Majie; Dettman, David L.

    2009-08-01

    The distribution and initial timing of the establishment of high surface elevations in the Rocky Mountains during the Early Cenozoic remain controversial despite the importance of these data in testing tectonic models for this region. We track the timing and distribution of high elevation in the Rocky Mountains during the Late Cretaceous-Early Eocene by examining annual and seasonal δ18O values of the ancient river water, which are calculated from the δ18O values of well-preserved freshwater bivalve shells. In the Powder River basin of the eastern Laramide province, the δ18O values of the ancient river water vary between - 23.0‰ and - 8.0‰ SMOW in both seasonal and annual records in the Late Paleocene-Early Eocene. The large variation suggests that the ancient rivers were fed yearly or seasonally by snowmelt from highlands of 4.5 ± 1.3 km. This can be explained by the existence of the Bighorn Mountains and Black Hills with a drainage pattern similar to the present in northeast Wyoming. The δ18O values of ancient river water along the front of the Sevier thrust belt generally follow a trend from lower values in north, - 14.2 ± 1.4‰ in the Early Paleocene Crazy Mountains basin, to higher values in south, - 11.1 ± 0.8‰ in the Late Paleocene Bighorn basin, and - 7.1 ± 1.6‰ in the Early Eocene Washakie basin. The variations within each basin are relatively small. These rivers most likely rise in the Sevier thrust belt, and may reflect highland elevation of 1-2 km. The δ18O values in the Alberta foreland and Williston basin are very low (- 20.5‰) in the Late Cretaceous, indicating the rivers were fed by snowmelt from the Canadian Rocky Mountains of 4.3 ± 1.0 km high. The attainment of high elevation in the eastern Laramide province prior to the western province could be explained by southwestward progression of back-thrusts soled into an earlier east-directed master detachment, which may be formed by the westward rollback of subducted shallow slab.

  17. Spatiotemporal alterations of presynaptic elements in the retina after high intraocular pressure

    Institute of Scientific and Technical Information of China (English)

    Jufang Huang; Lihong Zhou; Hui Wang; Jia Luo; Kun Xiong; Leping Zeng; Dan Chen

    2012-01-01

    A rat model of acute high intraocular pressure was established by injecting saline into the anterior chamber of the left eye. Synaptophysin expression was increased in the inner plexiform layer at 2 hours following injury, and was widely distributed in the outer plexiform layer at 3–7 days, and then decreased to the normal level at 14 days. This suggests that expression of this presynaptic functional protein experienced spatiotemporal alterations after elevation of intraocular pressure. There was no significant change in the fluorescence intensity and distribution pattern for synapse-associated protein 102 following elevated intraocular pressure. Synapse-associated protein 102 immunoreactivity was confined to the outer plexiform layer, while synaptophysin immunoreactivity spread into the outer plexiform layer and the outer nuclear layer at 3 and 7 days following injury. These alterations in presynaptic elements were not accompanied by changes in postsynaptic components.

  18. River sediment supply, sedimentation and transport of the highly turbid sediment plume in Malindi Bay, Kenya

    Institute of Scientific and Technical Information of China (English)

    JOHNSON U.Kitheka

    2013-01-01

    The paper presents results of a study on the sediment supply and movement of highly turbid sediment plume within Malindi Bay in the Northern region of the Kenya coast.The current velocities,tidal elevation,salinity and suspended sediment concentrations (TSSC)were measured in stations located within the bay using Aanderaa Recording Current Meter (RCM-9),Turbidity Sensor mounted on RCM-9,Divers Gauges and Aanderaa Temperature-Salinity Meter.The study established that Malindi Bay receives a high terrigenous sediment load amounting to 5.7 × 106 ton·yr-1.The river freshwater supply into the bay is highly variable ranging from 7 to 680 m3·s-1.The high flows that are > 150 m3·s-1 occurred in May during the South East Monsoon (SEM).Relatively low peak flows occurred in November during the North East Monsoon (NEM) but these were usually <70 m3·s-1.The discharge of highly turbidity river water into the bay in April and May occurs in a period of high intensity SEM winds that generate strong north flowing current that transports the river sediment plume northward.However,during the NEM,the river supply of turbid water is relatively low occurring in a period of relatively low intensity NEM winds that result in relatively weaker south flowing current that transports the sediment plume southward.The mechanism of advection of the sediment plume north or south of the estuary is mainly thought to be due to the Ekman transport generated by the onshore monsoon winds.Limited movement of the river sediment plume southward towards Ras Vasco Da Gama during NEM has ensured that the coral reef ecosystem in the northern parts of Malindi Marine National Park has not been completely destroyed by the influx of terrigenous sediments.However,to the north there is no coral reef ecosystem.The high sediment discharge into Malindi Bay can be attributed to land use change in the Athi-Sabaki River Basin in addition to rapid population increase which has led to clearance of forests to open land

  19. Structural alterations of the retinal microcirculation in the "prehypertensive" high- normal blood pressure state.

    Science.gov (United States)

    Grassi, Guido; Buzzi, Silvia; Dell'Oro, Raffaella; Mineo, Claudia; Dimitriadis, Kyriakos; Seravalle, Gino; Lonati, Laura; Cuspidi, Cesare

    2013-01-01

    The high-normal blood pressure (also known as prehypertension) is a clinical condition characterized by an increased cardiovascular risk as well as by the presence of target organ damage. This include an increased left ventricular mass, an endothelial dysfunction and an early renal functional and structural damage. Whether this is the case also for alterations of retinal vessels network, which are frequently detectable in established hypertension, is still largey undefined. The present paper, after discussing the main characteristics of the high-normal blood pressure state, will review the different approaches used throughout the years for assessing retinal microcirculatory network. Data collected by our group in subjects with high normal blood pressure will be also discussed, showing that arterial venular ratio values are reduced in this individuals with high-normal blood pressure and more so in established hypertension. These data indicate that retinal microvascular alterations 1) are of early appearance in the clinical course of hypertension and 2) are of frequent detection in the high-normal blood pressure state. The possible hemodynamic and non-hemodynamic mechanisms resposible for these structural alteations of the retinal microcirculation will be also discussed.

  20. High-resolution digital elevation model of lower Cowlitz and Toutle Rivers, adjacent to Mount St. Helens, Washington, based on an airborne lidar survey of October 2007

    Science.gov (United States)

    Mosbrucker, Adam

    2015-01-01

    The lateral blast, debris avalanche, and lahars of the May 18th, 1980, eruption of Mount St. Helens, Washington, dramatically altered the surrounding landscape. Lava domes were extruded during the subsequent eruptive periods of 1980–1986 and 2004–2008. More than three decades after the emplacement of the 1980 debris avalanche, high sediment production persists in the Toutle River basin, which drains the northern and western flanks of the volcano. Because this sediment increases the risk of flooding to downstream communities on the Toutle and lower Cowlitz Rivers, the U.S. Army Corps of Engineers (USACE), under the direction of Congress to maintain an authorized level of flood protection, continues to monitor and mitigate excess sediment in North and South Fork Toutle River basins to help reduce this risk and to prevent sediment from clogging the shipping channel of the Columbia River. From October 22–27, 2007, Watershed Sciences, Inc., under contract to USACE, collected high-precision airborne lidar (light detection and ranging) data that cover 273 square kilometers (105 square miles) of lower Cowlitz and Toutle River tributaries from the Columbia River at Kelso, Washington, to upper North Fork Toutle River (below the volcano's edifice), including lower South Fork Toutle River. These data provide a digital dataset of the ground surface, including beneath forest cover. Such remotely sensed data can be used to develop sediment budgets and models of sediment erosion, transport, and deposition. The U.S. Geological Survey (USGS) used these lidar data to develop digital elevation models (DEMs) of the study area. DEMs are fundamental to monitoring natural hazards and studying volcanic landforms, fluvial and glacial geomorphology, and surface geology. Watershed Sciences, Inc., provided files in the LASer (LAS) format containing laser returns that had been filtered, classified, and georeferenced. The USGS produced a hydro-flattened DEM from ground-classified points at

  1. Understanding Hydrological Regime Alterations Caused by dams: the Santiago River case in the Andean Region of the Amazon Basin.

    Science.gov (United States)

    Rosero-Lopez, D.; Flecker, A.; Walter, M. T.

    2016-12-01

    Water resources in South America have been clearly targeted as key sources for hydropower expansion over the next 30 years. Ecuador, among the most biologically diverse countries in the world, has the highest density of hydropower dams, either operational, under construction, or planned, in the Amazon Basin. Ecuador's ambitious plan to change its energy portfolio is conceived to satisfy the country's demand and to empower the country to be the region's first hydroelectric energy exporter. The Santiago watershed located in the southeast part of the country has 39 facilities either under construction or in operation. The Santiago River and its main tributaries (Zamora and Upano) are expected to be impounded by large dams over the next 10 years. In order to understand the magnitude and potential impacts of regional dam development on hydrological regimes, a 35-year historical data set of stream discharge was analyzed. We examined flow regimes for time series between the construction of each dam, starting with the oldest and largest built in 1982 up until the most recent dam built in 2005. Preliminary results indicate a systematic displacement in flow seasonality following post-dam compared to pre-dam conditions. There are also notable differences in the distributions of peaks and pulses in post-dam flows. The range of changes from these results shows that punctuated and cumulative impacts are related to the size of each new impoundment. These observations and their implications to the livelihoods, biota, and ecosystems services in the Santiago watershed need to be incorporated into a broader cost-benefit analysis of hydropower generation in the western Amazon Basin.

  2. Investigation of instabilities and rotation alteration in high beta KSTAR plasmas

    Science.gov (United States)

    Park, Y. S.; Sabbagh, S. A.; Ko, W. H.; Bak, J. G.; Berkery, J. W.; Bialek, J. M.; Choi, M. J.; Hahn, S. H.; In, Y. K.; Jardin, S. C.; Jeon, Y. M.; Kim, J.; Kwak, J. G.; Lee, S. G.; Oh, Y. K.; Park, H. K.; Yoon, S. W.; Yun, G. S.

    2017-01-01

    H-mode plasma operation of the Korea Superconducting Tokamak Advanced Research (KSTAR) device has been expanded to significantly surpass the ideal MHD no-wall beta limit. Plasmas with high normalized beta, βN, up to 4.3 have been achieved with reduced plasma internal inductance, li, to near 0.7, exceeding the computed n = 1 ideal no-wall limit by a factor of 1.6. Pulse lengths at maximum βN were extended to longer pulses by new, more rapid control. The stability of the observed m/n = 2/1 tearing mode that limited the achieved high βN is computed by the M3D-C1 code, and the effect of sheared toroidal rotation to tearing stability is examined. As a method to affect the mode stability in high βN plasmas, the non-resonant alteration of the rotation profile by non-axisymmetric magnetic fields has been used, enabling a study of the underlying neoclassical toroidal viscosity (NTV) physics and stability dependence on rotation. Non-axisymmetric field spectra were applied using in-vessel control coils (IVCCs) with varied n = 2 field configurations to alter the plasma toroidal rotation profile in high beta H-mode plasmas and to analyze their effects on the rotation. The rotation profile was significantly altered with rotation reduced by more than 60% without tearing activity or mode locking. To investigate the physical characteristics and scaling of the measured rotation braking by NTV, changes in the rotation profile are analytically examined in steady state. The expected NTV scaling with the square of the normalized applied field perturbation agrees with the measured profile change δB2.1-2.3. The NTV is also found to scale as Ti2.1-2.4, in general agreement with the low collisionality "1/ν" regime scaling of the NTV theory (TNTV-(1/ν) ∝ Ti2.5).

  3. Application of a land surface model for simulating river streamflow in high latitudes

    Science.gov (United States)

    Gusev, Yeugeniy; Nasonova, Olga; Dzhogan, Larissa

    2010-05-01

    Nowadays modelling runoff from the pan-Arctic river basins, which represents nearly 50% of water flow to the Arctic Ocean, is of great interest among hydrological modelling community because these regions are very sensitive to natural and anthropogenic impacts. This motivates the necessity of increase of the accuracy of hydrological estimations, runoff predictions, and water resources assessments in high latitudes. However, in these regions, observations required for model simulations (to specify model parameters and forcing inputs) are very scarce or even absent (especially this concerns land surface parameters). At the same time river discharge measurements are usually available that makes it possible to estimate model parameters by their calibration against measured discharge. Such a situation is typical of most of the northern basins of Russia. The major goal of the work is to reveal whether a physically-based land surface model (LSM) Soil Water - Atmosphere - Plants (SWAP) is able to reproduce snowmelt and rain driven daily streamflow in high latitudes (using poor input information) with the accuracy acceptable for hydrologic applications. Three river basins, located on the north of the European part of Russia, were chosen for investigation. They are the Mezen River basin (area: area: 78 000 km2), the Pechora River basin (area: 312 000 km2) and the Severnaya Dvina River basin (area: 348 000 km2). For modeling purposes the basins were presented, respectively, by 10, 57 and 62 one-degree computational grid boxes connected by river network. A priori estimation of the land surface parameters for each grid box was based on the global one-degree datasets prepared within the framework of the International Satellite Land-Surface Climatology Project Initiative II (ISLSCP) / the Second Global Soil Wetness Project (GSWP-2). Three versions of atmospheric forcing data prepared for the basins were based on: (1) NCEP/DOE reanalysis dataset; (2) NCEP/DOE reanalysis product

  4. In vivo high-resolution 7 Tesla MRI shows early and diffuse cortical alterations in CADASIL.

    Science.gov (United States)

    De Guio, François; Reyes, Sonia; Vignaud, Alexandre; Duering, Marco; Ropele, Stefan; Duchesnay, Edouard; Chabriat, Hugues; Jouvent, Eric

    2014-01-01

    Recent data suggest that early symptoms may be related to cortex alterations in CADASIL (Cerebral Autosomal-Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy), a monogenic model of cerebral small vessel disease (SVD). The aim of this study was to investigate cortical alterations using both high-resolution T2* acquisitions obtained with 7 Tesla MRI and structural T1 images with 3 Tesla MRI in CADASIL patients with no or only mild symptomatology (modified Rankin's scale ≤1 and Mini Mental State Examination (MMSE) ≥24). Complete reconstructions of the cortex using 7 Tesla T2* acquisitions with 0.7 mm isotropic resolution were obtained in 11 patients (52.1±13.2 years, 36% male) and 24 controls (54.8±11.0 years, 42% male). Seven Tesla T2* within the cortex and cortical thickness and morphology obtained from 3 Tesla images were compared between CADASIL and control subjects using general linear models. MMSE, brain volume, cortical thickness and global sulcal morphology did not differ between groups. By contrast, T2* measured by 7 Tesla MRI was significantly increased in frontal, parietal, occipital and cingulate cortices in patients after correction for multiple testing. These changes were not related to white matter lesions, lacunes or microhemorrhages in patients having no brain atrophy compared to controls. Seven Tesla MRI, by contrast to state of the art post-processing of 3 Tesla acquisitions, shows diffuse T2* alterations within the cortical mantle in CADASIL whose origin remains to be determined.

  5. Alteration Development of the Simulated HLW Glass at High Temperature in Beishan Underground Water

    Directory of Open Access Journals (Sweden)

    Zhentao Zhang

    2012-01-01

    Full Text Available The simulated HLW glass was found to be altered in Beishan underground water at high temperature in two different stages starting with slow leaching of the immobilized elements for a period followed by a sharp degradation of the glass matrix. Immersed at 150°C in Beishan underground water with glass-surface-area-to-solution-volume ratio of 6000 m-1, the glass was alterated rapidly with the sharp release of B, Na, Li, Cs, and Mo from the cold HLW glass after a stable period of 180 days. The glass was degraded up to 73.6% for the immersion period of 730 days resulting in the release of Mo and Cs up to 73.6% and 2.7% from the glass, respectively. With the alteration underway, new minerals were identified to be zeolite P, mordenite, nontronite, dickite, okonite, quartz, saponite, and tincalconite. However, at low temperature of 90°C, the glass was very stable with limited leaching of Na, B, and Li.

  6. Altered autophagy and sympathetic innervation in salivary glands from high-fat diet mice.

    Science.gov (United States)

    de Carvalho, Polliane Morais; Gavião, Maria Beatriz Duarte; Carpenter, Guy Howard

    2017-03-01

    to investigate the effects of a high fat diet (HFD) on salivary glands in vivo, in a mouse model. In particular, whether it will induce the appearance of fat cells in salivary glands, alterations related to autophagy, mTOR pathway and sympathetic innervation. 27 adult female ICR mice were separated in six groups. Three groups fed with (HFD) containing 55% fat, for one, two and three month and another three groups fed with normal diet (2.7% of fat), for the same time periods. The submandibular glands and liver were dissected and part homogenized for protein analyses and part fixed in formalin for histological analyses. After three months the HFD fed mice total body weight fold change increased compared to controls. The Oil Red O staining showed no fat cells deposit in salivary gland however a large increase was observed in liver after three months of HFD. Adiponectin levels were significantly decreased in the HFD group after three months. The group fed with HFD for three months showed increased conversion of the LC3 autophagy marker in salivary gland. mTOR showed no activation regarding the time point studied. Tyrosine hydroxylase significantly decreased after two and three month of HFD. HFD caused several changes after three months however the earliest change was noticed after two months regarding sympathetic innervation. This suggests neural alteration may drive other diet induced changes in salivary glands. These early changes may be the starting point for longer term alterations of salivary glands with alterations in diet. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Effects of high salinity wastewater discharges on unionid mussels in the Allegheny River, Pennsylvania

    Science.gov (United States)

    Kathleen Patnode,; Hittle, Elizabeth A.; Robert Anderson,; Lora Zimmerman,; Fulton, John W.

    2015-01-01

    We examined the effect of high salinity wastewater (brine) from oil and natural gas drilling on freshwater mussels in the Allegheny River, Pennsylvania, during 2012. Mussel cages (N = 5 per site) were deployed at two sites upstream and four sites downstream of a brine treatment facility on the Allegheny River. Each cage contained 20 juvenile northern riffleshell mussels Epioblasma torulosa rangiana). Continuous specific conductance and temperature data were recorded by water quality probes deployed at each site. To measure the amount of mixing throughout the entire study area, specific conductance surveys were completed two times during low-flow conditions along transects from bank to bank that targeted upstream (reference) reaches, a municipal wastewater treatment plant discharge upstream of the brine-facility discharge, the brine facility, and downstream reaches. Specific conductance data indicated that high specific conductance water from the brine facility (4,000–12,000 µS/cm; mean 7,846) compared to the reference reach (103–188 µS/cm; mean 151) is carried along the left descending bank of the river and that dilution of the discharge via mixing does not occur until 0.5 mi (805 m) downstream. Juvenile northern riffleshell mussel survival was severely impaired within the high specific conductance zone (2 and 34% at and downstream of the brine facility, respectively) and at the municipal wastewater treatment plant (21%) compared to background (84%). We surveyed native mussels (family Unionidae) at 10 transects: 3 upstream, 3 within, and 4 downstream of the high specific conductance zone. Unionid mussel abundance and diversity were lower for all transects within and downstream of the high conductivity zone compared to upstream. The results of this study clearly demonstrate in situ toxicity to juvenile northern riffleshell mussels, a federally endangered species, and to the native unionid mussel assemblage located downstream of a brine discharge to the

  8. High Fat Diet Alters Lactation Outcomes: Possible Involvement of Inflammatory and Serotonergic Pathways

    OpenAIRE

    Hernandez, Laura L; Grayson, Bernadette E.; Ekta Yadav; Seeley, Randy J.; Nelson D Horseman

    2012-01-01

    Delay in the onset of lactogenesis has been shown to occur in women who are obese, however the mechanism altered within the mammary gland causing the delay remains unknown. Consumption of high fat diets (HFD) has been previously determined to result decreased litters and litter numbers in rodent models due to a decrease in fertility. We examined the effects of feeding a HFD (60% kcal from fat) diet versus a low-fat diet (LFD; 10% kcal from fat) to female Wistar rats on lactation outcomes. Fee...

  9. The Significance of Acid Alteration in the Los Humeros High-Temperature Geothermal Field, Puebla, Mexico.

    Science.gov (United States)

    Elders, W. A.; Izquierdo, G.

    2014-12-01

    The Los Humeros geothermal field is a high-enthalpy hydrothermal system with more than 40 drilled deep wells, mostly producing high steam fractions at > 300oC. However, although it has a large resource potential, low permeability and corrosive acid fluids have hampered development so that it currently has an installed electrical generating capacity of only 40 MWe. The widespread production of low pH fluids from the reservoir is inconsistent with the marked absence in the reservoir rocks of hydrothermal minerals typical of acid alteration. Instead the hydrothermal alteration observed is typical of that due to neutral to alkaline pH waters reacting with the volcanic rocks of the production zones. Thus it appears that since the reservoir has recently suffered a marked drop in fluid pressure and is in process of transitioning from being water-dominated to being vapor-dominated. However sparse examples of acid leaching are observed locally at depths of about 2 km in the form of bleached, intensely silicified zones, in low permeability and very hot (>350oC) parts of reservoir. Although these leached rocks retain their primary volcanic and pyroclastic textures, they are altered almost entirely to microcrystalline quartz, with some relict pseudomorphs of plagioclase phenocrysts and traces of earlier-formed hydrothermal chlorite and pyrite. These acid-altered zones are usually only some tens of meters thick and deeper rocks lack such silicification. The acid fluids responsible for their formation could either be magmatic volatiles, or could be formed during production (e.g. reaction of water and salts forming hydrogen chloride by hydrolysis at high temperatures). The very high boron content of the fluids produced by the Los Humeros wells suggests that their ultimate source is most likely magmatic gases. However, these acid gases did not react widely with the rocks. We suggest that the silicified zones are forming locally where colder descending waters are encountering

  10. Modeling of nitrobenzene in the river with ice process in high-latitude regions

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    To deal with the accidental release of pollutants into frozen rivers, those rivers in high-latitude regions should be treated differently. Pollutants could be stored up in the ice and released when the ice melts in spring, perhaps resulting in secondary pollution in the river. A water quality model of nitrobenzene has been developed in this paper to assess the influence on river water quality due to the freezing process. The model is made up of three modules: thermodynamic module, hydrodynamic module and water quality module. The thermodynamic module considers the complex heat exchange processes between the water body and the atmosphere, the water body and the river bed, the water body and the ice cover, and so on. The growth of the ice cover is simulated in a simplified form whilst taking consideration of heat balance. The hydrodynamic model uses the Saint-Venant equations in non-constant flow and the influence of the ice cover is measured. The degradation, diffusion, absorption and desorption, and the influence of the freezing process are incorporated in the water quality module and the concept of Con- tinuously Stirred Tanked Reactors (CSTRs) model is applied in the model construction. The model has been applied to supporting the management of accidental pollution in the Songhua River. The model was calibrated and validated with monitored data. Regional sensitivity analysis based on a task-based Hornberger-Spear-Young (HSY) algorithm was carried out to examine the model structure and the re- sult showed that the model could describe the system very well. Then the conditioned model was ap- plied to predicting the concentration of nitrobenzene in the water when the ice melted in spring. The predictive result showed that the release of pollutants in the ice could lead to an increase in the con- centration of pollutants in the water, but the increase would be very small because there was only a small amount of pollutants stored in the ice. A secondary pollution

  11. Modeling of nitrobenzene in the river with ice process in high-latitude regions

    Institute of Scientific and Technical Information of China (English)

    SUN PengCheng; ZENG SiYu; CHEN JiNing

    2009-01-01

    To deal with the accidental release of pollutants into frozen rivers,those rivers in high-latitude regions should be treated differently.Pollutants could be stored up in the ice and released when the ice melts in spring,perhaps resulting in secondary pollution in the river.A water quality model of nitrobenzene has been developed in this paper to assess the influence on river water quality due to the freezing process.The model is made up of three modules:thermodynamic module,hydrodynamic module and water quality module.The thermodynamic module considers the complex heat exchange processes between the water body and the atmosphere,the water body and the river bed,the water body and the ice cover,and so on.The growth of the ice cover is simulated in a simplified form whilst taking consideration of heat balance.The hydrodynamic model uses the Saint-Venant equations in non-constant flow and the influence of the ice cover is measured.The degradation,diffusion,absorption and desorption,and the influence of the freezing process are incorporated in the water quality module and the concept of Continuously Stirred Tanked Reactors (CSTRs) model is applied in the model construction.The model has been applied to supporting the management of accidental pollution in the Songhua River.The model was calibrated and validated with monitored data.Regional sensitivity analysis based on a task-based Hornberger-Spear-Young (HSY) algorithm was carried out to examine the model structure and the result showed that the model could describe the system very well.Then the conditioned model was applied to predicting the concentration of nitrobenzene in the water when the ice melted in spring.The predictive result showed that the release of pollutants in the ice could lead to an increase in the concentration of pollutants in the water,but the increase would be very small because there was only a small amount of pollutants stored in the ice.A secondary pollution could therefore be avoided

  12. The salivary microbiome is altered in the presence of a high salivary glucose concentration

    Science.gov (United States)

    Hartman, Mor-Li; Shi, Ping; Hasturk, Hatice; Yaskell, Tina; Vargas, Jorel; Song, Xiaoqing; Cugini, Maryann; Barake, Roula; Alsmadi, Osama; Al-Mutawa, Sabiha; Ariga, Jitendra; Soparkar, Pramod; Behbehani, Jawad; Behbehani, Kazem

    2017-01-01

    Background Type II diabetes (T2D) has been associated with changes in oral bacterial diversity and frequency. It is not known whether these changes are part of the etiology of T2D, or one of its effects. Methods We measured the glucose concentration, bacterial counts, and relative frequencies of 42 bacterial species in whole saliva samples from 8,173 Kuwaiti adolescents (mean age 10.00 ± 0.67 years) using DNA probe analysis. In addition, clinical data related to obesity, dental caries, and gingivitis were collected. Data were compared between adolescents with high salivary glucose (HSG; glucose concentration ≥ 1.0 mg/d, n = 175) and those with low salivary glucose (LSG, glucose concentration gingivitis in the study population. The overall salivary bacterial load in saliva decreased with increasing salivary glucose concentration. Under HSG conditions, the bacterial count for 35 (83%) of 42 species was significantly reduced, and relative bacterial frequencies in 27 species (64%) were altered, as compared with LSG conditions. These alterations were stronger predictors of high salivary glucose than measures of oral disease, obesity, sleep or fitness. Conclusions HSG was associated with a reduction in overall bacterial load and alterations to many relative bacterial frequencies in saliva when compared with LSG in samples from adolescents. We propose that hyperglycemia due to obesity and/or T2D results in HSG and subsequent acidification of the oral environment, leading to a generalized perturbation in the oral microbiome. This suggests a basis for the observation that hyperglycemia is associated with an increased risk of dental erosion, dental caries, and gingivitis. We conclude that HSG in adolescents may be predicted from salivary microbial diversity or frequency, and that the changes in the oral microbial composition seen in adolescents with developing metabolic disease may the consequence of hyperglycemia. PMID:28249034

  13. Altered gene expression in highly purified enterocytes from patients with active coeliac disease

    Directory of Open Access Journals (Sweden)

    Jackson John

    2008-08-01

    Full Text Available Abstract Background Coeliac disease is a multifactorial inflammatory disorder of the intestine caused by ingestion of gluten in genetically susceptible individuals. Genes within the HLA-DQ locus are considered to contribute some 40% of the genetic influence on this disease. However, information on other disease causing genes is sparse. Since enterocytes are considered to play a central role in coeliac pathology, the aim of this study was to examine gene expression in a highly purified isolate of these cells taken from patients with active disease. Epithelial cells were isolated from duodenal biopsies taken from five coeliac patients with active disease and five non-coeliac control subjects. Contaminating T cells were removed by magnetic sorting. The gene expression profile of the cells was examined using microarray analysis. Validation of significantly altered genes was performed by real-time RT-PCR and immunohistochemistry. Results Enterocyte suspensions of high purity (98–99% were isolated from intestinal biopsies. Of the 3,800 genes investigated, 102 genes were found to have significantly altered expression between coeliac disease patients and controls (p Conclusion This study provides a profile of the molecular changes that occur in the intestinal epithelium of coeliac patients with active disease. Novel candidate genes were revealed which highlight the contribution of the epithelial cell to the pathogenesis of coeliac disease.

  14. Switching adolescent high-fat diet to adult control diet restores neurocognitive alterations

    Directory of Open Access Journals (Sweden)

    Chloe Boitard

    2016-11-01

    Full Text Available In addition to metabolic and cardiovascular disorders, obesity is associated with adverse cognitive and emotional outcomes. Its growing prevalence in adolescents is particularly alarming since this is a period of ongoing maturation for brain structures (including the hippocampus and amygdala and for the hypothalamic-pituitary-adrenal (HPA stress axis, which is required for cognitive and emotional processing. We recently demonstrated that adolescent, but not adult, high-fat diet (HF exposure leads to impaired hippocampal function and enhanced amygdala function through HPA axis alteration (Boitard et al., 2014; Boitard et al., 2012; Boitard et al., 2015. Here, we assessed whether the effects of adolescent HF consumption on brain function are permanent or reversible. After adolescent exposure to HF, switching to a standard chow diet restored levels of hippocampal neurogenesis and normalized enhanced HPA axis reactivity, amygdala activity and avoidance memory. Therefore, while the adolescent period is highly vulnerable to the deleterious effects of diet-induced obesity, adult exposure to a standard diet appears sufficient to reverse alterations of brain function.

  15. Influence of high levels of total suspended solids on measurement of COD and BOD in the Yellow River, China.

    Science.gov (United States)

    Jingsheng, Chen; Tao, Yu; Ongley, Edwin

    2006-05-01

    Using the Yellow River, China, the study explores the problem of the use of COD and BOD(5) as water quality management parameters in the presence of very high levels of suspended sediment (TSS) that characterize this river. Although the amount of natural organic matter per unit of suspended sediment of the Yellow River is not high, the very high concentration of mineral sediment in the Yellow River results in a large concentration of organic matter, which artificially inflates the laboratory values of COD and, as a consequent, leads to greatly exaggerated reports of pollution of the Yellow River. BOD(5) can more accurately reflect the pollution of the Yellow River than COD; however, measured values of BOD under-report the actual values due to settling of the sediment in the incubation chamber resulting in values that are 21.6--38.3% less than the actual values. Therefore corrections are required for laboratory COD and BOD values so that the values are not artifacts of the sediment regime. Our work provides new insight into this phenomenon and demonstrates how correction factors may be determined and used with pollution data. Our work also suggests that the actual pollution levels of the Yellow River are probably not as high as reported by monitoring agencies.

  16. Physiological and molecular alterations in plants exposed to high [CO2] under phosphorus stress.

    Science.gov (United States)

    Pandey, Renu; Zinta, Gaurav; AbdElgawad, Hamada; Ahmad, Altaf; Jain, Vanita; Janssens, Ivan A

    2015-01-01

    Atmospheric [CO2] has increased substantially in recent decades and will continue to do so, whereas the availability of phosphorus (P) is limited and unlikely to increase in the future. P is a non-renewable resource, and it is essential to every form of life. P is a key plant nutrient controlling the responsiveness of photosynthesis to [CO2]. Increases in [CO2] typically results in increased biomass through stimulation of net photosynthesis, and hence enhance the demand for P uptake. However, most soils contain low concentrations of available P. Therefore, low P is one of the major growth-limiting factors for plants in many agricultural and natural ecosystems. The adaptive responses of plants to [CO2] and P availability encompass alterations at morphological, physiological, biochemical and molecular levels. In general low P reduces growth, whereas high [CO2] enhances it particularly in C3 plants. Photosynthetic capacity is often enhanced under high [CO2] with sufficient P supply through modulation of enzyme activities involved in carbon fixation such as ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). However, high [CO2] with low P availability results in enhanced dry matter partitioning towards roots. Alterations in below-ground processes including root morphology, exudation and mycorrhizal association are influenced by [CO2] and P availability. Under high P availability, elevated [CO2] improves the uptake of P from soil. In contrast, under low P availability, high [CO2] mainly improves the efficiency with which plants produce biomass per unit P. At molecular level, the spatio-temporal regulation of genes involved in plant adaptation to low P and high [CO2] has been studied individually in various plant species. Genome-wide expression profiling of high [CO2] grown plants revealed hormonal regulation of biomass accumulation through complex transcriptional networks. Similarly, differential transcriptional regulatory networks are involved in P

  17. In vivo high-resolution 7 Tesla MRI shows early and diffuse cortical alterations in CADASIL.

    Directory of Open Access Journals (Sweden)

    François De Guio

    Full Text Available Recent data suggest that early symptoms may be related to cortex alterations in CADASIL (Cerebral Autosomal-Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy, a monogenic model of cerebral small vessel disease (SVD. The aim of this study was to investigate cortical alterations using both high-resolution T2* acquisitions obtained with 7 Tesla MRI and structural T1 images with 3 Tesla MRI in CADASIL patients with no or only mild symptomatology (modified Rankin's scale ≤1 and Mini Mental State Examination (MMSE ≥24.Complete reconstructions of the cortex using 7 Tesla T2* acquisitions with 0.7 mm isotropic resolution were obtained in 11 patients (52.1±13.2 years, 36% male and 24 controls (54.8±11.0 years, 42% male. Seven Tesla T2* within the cortex and cortical thickness and morphology obtained from 3 Tesla images were compared between CADASIL and control subjects using general linear models.MMSE, brain volume, cortical thickness and global sulcal morphology did not differ between groups. By contrast, T2* measured by 7 Tesla MRI was significantly increased in frontal, parietal, occipital and cingulate cortices in patients after correction for multiple testing. These changes were not related to white matter lesions, lacunes or microhemorrhages in patients having no brain atrophy compared to controls.Seven Tesla MRI, by contrast to state of the art post-processing of 3 Tesla acquisitions, shows diffuse T2* alterations within the cortical mantle in CADASIL whose origin remains to be determined.

  18. Different methods to alter surface morphology of high aspect ratio structures

    Energy Technology Data Exchange (ETDEWEB)

    Leber, M., E-mail: moritz.leber@utah.edu [Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT (United States); Shandhi, M.M.H. [Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT (United States); Hogan, A. [Blackrock Microsystems, Salt Lake City, UT (United States); Solzbacher, F. [Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT (United States); Bhandari, R.; Negi, S. [Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT (United States); Blackrock Microsystems, Salt Lake City, UT (United States)

    2016-03-01

    Graphical abstract: Surface engineering of high aspect ratio silicon structures. - Highlights: • Multiple roughening techniques for high aspect ratio devices were investigated. • Modification of surface morphology of high aspect ratio silicon devices (1:15). • Decrease of 76% in impedance proves significant increase in surface area. - Abstract: In various applications such as neural prostheses or solar cells, there is a need to alter the surface morphology of high aspect ratio structures so that the real surface area is greater than geometrical area. The change in surface morphology enhances the devices functionality. One of the applications of altering the surface morphology is of neural implants such as the Utah electrode array (UEA) that communicate with single neurons by charge injection induced stimulation or by recording electrical neural signals. For high selectivity between single cells of the nervous system, the electrode surface area is required to be as small as possible, while the impedance is required to be as low as possible for good signal to noise ratios (SNR) during neural recording. For stimulation, high charge injection and charge transfer capacities of the electrodes are required, which increase with the electrode surface. Traditionally, researchers have worked with either increasing the roughness of the existing metallization (platinum grey, black) or other materials such as Iridium Oxide and PEDOT. All of these previously investigated methods lead to more complicated metal deposition processes that are difficult to control and often have a critical impact on the mechanical properties of the metal films. Therefore, a modification of the surface underneath the electrode's coating will increase its surface area while maintaining the standard and well controlled metal deposition process. In this work, the surfaces of the silicon micro-needles were engineered by creating a defined microstructure on the electrodes surface using several

  19. Alterations of mineral elements in osteoblast during differentiation under hypo, moderate and high static magnetic fields.

    Science.gov (United States)

    Zhang, Jian; Ding, Chong; Shang, Peng

    2014-12-01

    Static magnetic fields (SMFs) can enhance the ability of bone formation by osteoblast and is a potential physical therapy to bone disorders and the maintenance of bone health. But, the mechanism is not clear yet. Certain mineral elements including macro and trace elements are essential for normal bone metabolism. Deficiency of these elements can cause severe bone disorders including osteoporosis. However, there are few reports regarding the role of mineral elements in the regulation of bone formation under SMFs. In this study, hypomagnetic field (HyMF) of 500 nT, moderate SMF (MMF) of 0.2 T, and high SMF (HiMF) of 16 T were used to investigate the effects of SMFs on mineral element (calcium, copper, iron, magnesium, manganese, and zinc) alteration of MC3T3-E1 cells during osteoblast mineralization. The results showed that osteoblasts in differentiation accumulated more mineral elements than non-differentiated cell cultures. Furthermore, HyMF reduced osteoblast differentiation but did not affect mineral elements levels compared with control of geomagnetic field. MMF decreased osteoblast differentiation with elevated iron content. HiMF enhanced osteoblast differentiation and increased all the mineral contents except copper. It is suggested that the altered potential of osteoblast differentiation under SMFs may partially due to the involvement of different mineral elements.

  20. Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high let radiation

    Science.gov (United States)

    Hei, T. K.; Zhao, Y. L.; Roy, D.; Piao, C. Q.; Calaf, G.; Hall, E. J.

    Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/μm alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three ( DCC, DNA-PK and p21 CIPI) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.

  1. Acute high-intensity sound exposure alters responses of place cells in hippocampus.

    Science.gov (United States)

    Goble, T J; Møller, A R; Thompson, L T

    2009-07-01

    Overstimulation is known to activate neural plasticity in the auditory nervous system causing changes in function and re-organization. It has been shown earlier that overstimulation using high-intensity noise or tones can induce signs of tinnitus. Here we show in studies in rats that overstimulation causes changes in the way place cells of the hippocampus respond as rats search for rewards in a spatial maze. In familiar environments, a subset of hippocampal pyramidal neurons, known as place cells, respond when the animal moves through specific locations but are relatively silent in others. This place-field activity (i.e. location-specific firing) is stable in a fixed environment. The present study shows that activation of neural plasticity through overstimulation by sound can alter the response of these place cells. Rats implanted with chronic drivable dorsal hippocampal tetrodes (four microelectrodes) were assessed for stable single-unit place-field responses that were extracted from multiunit responses using NeuroExplorer computer spike-sorting software. Rats then underwent either 30 min exposure to a 4 kHz tone at 104 dB SPL or a control period in the same sound chamber. The place-field activity was significantly altered after sound exposure showing that plastic changes induced by overstimulation are not limited to the auditory nervous system but extend to other parts of the CNS, in this case to the hippocampus, a brain region often studied in the context of plasticity.

  2. Saharan dust inputs and high UVR levels jointly alter the metabolic balance of marine oligotrophic ecosystems

    Science.gov (United States)

    Cabrerizo, Marco J.; Medina-Sánchez, Juan Manuel; González-Olalla, Juan Manuel; Villar-Argaiz, Manuel; Carrillo, Presentación

    2016-10-01

    The metabolic balance of the most extensive bioma on the Earth is a controversial topic of the global-change research. High ultraviolet radiation (UVR) levels by the shoaling of upper mixed layers and increasing atmospheric dust deposition from arid regions may unpredictably alter the metabolic state of marine oligotrophic ecosystems. We performed an observational study across the south-western (SW) Mediterranean Sea to assess the planktonic metabolic balance and a microcosm experiment in two contrasting areas, heterotrophic nearshore and autotrophic open sea, to test whether a combined UVR × dust impact could alter their metabolic balance at mid-term scales. We show that the metabolic state of oligotrophic areas geographically varies and that the joint impact of UVR and dust inputs prompted a strong change towards autotrophic metabolism. We propose that this metabolic response could be accentuated with the global change as remote-sensing evidence shows increasing intensities, frequencies and number of dust events together with variations in the surface UVR fluxes on SW Mediterranean Sea. Overall, these findings suggest that the enhancement of the net carbon budget under a combined UVR and dust inputs impact could contribute to boost the biological pump, reinforcing the role of the oligotrophic marine ecosystems as CO2 sinks.

  3. Radiation-Induced Epigenetic Alterations after Low and High LET Irradiations

    Energy Technology Data Exchange (ETDEWEB)

    Aypar, Umut; Morgan, William F.; Baulch, Janet E.

    2011-02-01

    Epigenetics, including DNA methylation and microRNA (miRNA) expression, could be the missing link in understanding the delayed, non-targeted effects of radiation including radiationinduced genomic instability (RIGI). This study tests the hypothesis that irradiation induces epigenetic aberrations, which could eventually lead to RIGI, and that the epigenetic aberrations induced by low linear energy transfer (LET) irradiation are different than those induced by high LET irradiations. GM10115 cells were irradiated with low LET x-rays and high LET iron (Fe) ions and evaluated for DNA damage, cell survival and chromosomal instability. The cells were also evaluated for specific locus methylation of nuclear factor-kappa B (NFκB), tumor suppressor in lung cancer 1 (TSLC1) and cadherin 1 (CDH1) gene promoter regions, long interspersed nuclear element 1 (LINE-1) and Alu repeat element methylation, CpG and non-CpG global methylation and miRNA expression levels. Irradiated cells showed increased micronucleus induction and cell killing immediately following exposure, but were chromosomally stable at delayed times post-irradiation. At this same delayed time, alterations in repeat element and global DNA methylation and miRNA expression were observed. Analyses of DNA methylation predominantly showed hypomethylation, however hypermethylation was also observed. MiRNA shown to be altered in expression level after x-ray irradiation are involved in chromatin remodeling and DNA methylation. Different and higher incidence of epigenetic changes were observed after exposure to low LET x-rays than high LET Fe ions even though Fe ions elicited more chromosomal damage and cell killing. This study also shows that the irradiated cells acquire epigenetic changes even though they are chromosomally stable suggesting that epigenetic aberrations may arise in the cell without initiating RIGI.

  4. Examination of Poststroke Alteration in Motor Unit Firing Behavior Using High-Density Surface EMG Decomposition.

    Science.gov (United States)

    Li, Xiaoyan; Holobar, Ales; Gazzoni, Marco; Merletti, Roberto; Rymer, William Zev; Zhou, Ping

    2015-05-01

    Recent advances in high-density surface electromyogram (EMG) decomposition have made it a feasible task to discriminate single motor unit activity from surface EMG interference patterns, thus providing a noninvasive approach for examination of motor unit control properties. In the current study, we applied high-density surface EMG recording and decomposition techniques to assess motor unit firing behavior alterations poststroke. Surface EMG signals were collected using a 64-channel 2-D electrode array from the paretic and contralateral first dorsal interosseous (FDI) muscles of nine hemiparetic stroke subjects at different isometric discrete contraction levels between 2 to 10 N with a 2 N increment step. Motor unit firing rates were extracted through decomposition of the high-density surface EMG signals and compared between paretic and contralateral muscles. Across the nine tested subjects, paretic FDI muscles showed decreased motor unit firing rates compared with contralateral muscles at different contraction levels. Regression analysis indicated a linear relation between the mean motor unit firing rate and the muscle contraction level for both paretic and contralateral muscles (p < 0.001), with the former demonstrating a lower increment rate (0.32 pulses per second (pps)/N) compared with the latter (0.67 pps/N). The coefficient of variation (averaged over the contraction levels) of the motor unit firing rates for the paretic muscles (0.21 ± 0.012) was significantly higher than for the contralateral muscles (0.17 ± 0.014) (p < 0.05). This study provides direct evidence of motor unit firing behavior alterations poststroke using surface EMG, which can be an important factor contributing to hemiparetic muscle weakness.

  5. High psychosis liability is associated with altered autonomic balance during exposure to Virtual Reality social stressors.

    Science.gov (United States)

    Counotte, Jacqueline; Pot-Kolder, Roos; van Roon, Arie M; Hoskam, Olivier; van der Gaag, Mark; Veling, Wim

    2017-06-01

    Social stressors are associated with an increased risk of psychosis. Stress sensitisation is thought to be an underlying mechanism and may be reflected in an altered autonomic stress response. Using an experimental Virtual Reality design, the autonomic stress response to social stressors was examined in participants with different liability to psychosis. Fifty-five patients with recent onset psychotic disorder, 20 patients at ultra-high risk for psychosis, 42 siblings of patients with psychosis and 53 controls were exposed to social stressors (crowdedness, ethnic minority status and hostility) in a Virtual Reality environment. Heart rate variability parameters and skin conductance levels were measured at baseline and during Virtual Reality experiments. High psychosis liability groups had significantly increased heart rate and decreased heart rate variability compared to low liability groups both at baseline and during Virtual Reality experiments. Both low frequency (LF) and high frequency (HF) power were reduced, while the LF/HF ratio was similar between groups. The number of virtual social stressors significantly affected heart rate, HF, LF/HF and skin conductance level. There was no interaction between psychosis liability and amount of virtual social stress. High liability to psychosis is associated with decreased parasympathetic activity in virtual social environments, which reflects generally high levels of arousal, rather than increased autonomic reactivity to social stressors. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. About the high flow regime of the rivers of Kosovo and Metohia

    Directory of Open Access Journals (Sweden)

    Živković Nenad

    2009-01-01

    Full Text Available The examples from Kosovo and Metohia attempted to point to some problems in the domain of hydrogeographic regionalization. The river water regime, especially the phase of high flows which marks this regime, has been the topic of almost all researches which treat water resources of drainage basins. However, the thing that has not been achieved till now is the unique solution by which the classification of rivers would be made according to this feature. On this example it has been shown that even some older methods, based on genetic analysis of hydrograms and of global type, as well as some recent ones, with lot of quantitative entry and regional approaches, cannot with certainty answer all the challenges which river regimes bring with themselves. This work shows that apart from climate, orographic and physiognomic features of drainage basins, the periods of data processing and the analysis of individual intra-annual series of discharges are very important as well. Discretization on time periods shorter than one month, as well as elimination of the extreme values of discharges in the longtime series is recommended for the future research.

  7. Is high-resolution inverse characterization of heterogeneous river bed hydraulic conductivities needed and possible?

    Directory of Open Access Journals (Sweden)

    W. Kurtz

    2013-10-01

    Full Text Available River–aquifer exchange fluxes influence local and regional water balances and affect groundwater and river water quality and quantity. Unfortunately, river–aquifer exchange fluxes tend to be strongly spatially variable, and it is an open research question to which degree river bed heterogeneity has to be represented in a model in order to achieve reliable estimates of river–aquifer exchange fluxes. This research question is addressed in this paper with the help of synthetic simulation experiments, which mimic the Limmat aquifer in Zurich (Switzerland, where river–aquifer exchange fluxes and groundwater management activities play an important role. The solution of the unsaturated–saturated subsurface hydrological flow problem including river–aquifer interaction is calculated for ten different synthetic realities where the strongly heterogeneous river bed hydraulic conductivities (L are perfectly known. Hydraulic head data (100 in the default scenario are sampled from the synthetic realities. In subsequent data assimilation experiments, where L is unknown now, the hydraulic head data are used as conditioning information, with the help of the ensemble Kalman filter (EnKF. For each of the ten synthetic realities, four different ensembles of L are tested in the experiments with EnKF; one ensemble estimates high-resolution L fields with different L values for each element, and the other three ensembles estimate effective L values for 5, 3 or 2 zones. The calibration of higher-resolution L fields (i.e. fully heterogeneous or 5 zones gives better results than the calibration of L for only 3 or 2 zones in terms of reproduction of states, stream–aquifer exchange fluxes and parameters. Effective L for a limited number of zones cannot always reproduce the true states and fluxes well and results in biased estimates of net exchange fluxes between aquifer and stream. Also in case only 10 head data are used for conditioning, the high

  8. Retinal Targets ALDH Positive Cancer Stem Cell and Alters the Phenotype of Highly Metastatic Osteosarcoma Cells

    Directory of Open Access Journals (Sweden)

    Xiaodong Mu

    2015-01-01

    Full Text Available Aldehyde dehydrogenase (ALDH is a cancer stem cell marker. Retinoic acid has antitumor properties, including the induction of apoptosis and inhibition of proliferation. Retinal, the precursor of retinoic acid, can be oxidized to retinoic acid by dehydrogenases, including ALDH. We hypothesized that retinal could potentially be transformed to retinoic acid with higher efficiency by cancer stem cells, due to the higher ALDH activity. We previously observed that ALDH activity is greater in highly metastatic K7M2 osteosarcoma (OS cells than in nonmetastatic K12 OS cells. We also demonstrated that ALDH activity correlates with clinical metastases in bone sarcoma patients, suggesting that ALDH may be a therapeutic target specific to cells with high metastatic potential. Our current results demonstrated that retinal preferentially affected the phenotypes of ALDH-high K7M2 cells in contrast to ALDH-low K12 cells, which could be mediated by the more efficient transformation of retinal to retinoic acid by ALDH in K7M2 cells. Retinal treatment of highly metastatic K7M2 cells decreased their proliferation, invasion capacity, and resistance to oxidative stress. Retinal altered the expression of metastasis-related genes. These observations indicate that retinal may be used to specifically target metastatic cancer stem cells in OS.

  9. High-intensity statin therapy alters the natural history of diabetic coronary atherosclerosis: insights from SATURN.

    Science.gov (United States)

    Stegman, Brian; Puri, Rishi; Cho, Leslie; Shao, Mingyuan; Ballantyne, Christie M; Barter, Phillip J; Chapman, M John; Erbel, Raimund; Libby, Peter; Raichlen, Joel S; Uno, Kiyoko; Kataoka, Yu; Nissen, Steven E; Nicholls, Stephen J

    2014-11-01

    Although statins can induce coronary atheroma regression, this benefit has yet to be demonstrated in diabetic individuals. We tested the hypothesis that high-intensity statin therapy may promote coronary atheroma regression in patients with diabetes. The Study of Coronary Atheroma by Intravascular Ultrasound: Effect of Rosuvastatin Versus Atorvastatin (SATURN) used serial intravascular ultrasound measures of coronary atheroma volume in patients treated with rosuvastatin 40 mg or atorvastatin 80 mg for 24 months. This analysis compared changes in biochemistry and coronary percent atheroma volume (PAV) in patients with (n = 159) and without (n = 880) diabetes. At baseline, patients with diabetes had lower LDL cholesterol (LDL-C) and HDL cholesterol (HDL-C) levels but higher triglyceride and CRP levels compared with patients without diabetes. At follow-up, diabetic patients had lower levels of LDL-C (61.0 ± 20.5 vs. 66.4 ± 22.9 mg/dL, P = 0.01) and HDL-C (46.3 ± 10.6 vs. 49.9 ± 12.0 mg/dL, P 70 mg/dL (-0.31 ± 0.23 vs. -1.01 ± 0.21%, P = 0.03) but similar when LDL-C levels were ≤70 mg/dL (-1.09 ± 0.16 vs. -1.24 ± 0.16%, P = 0.50). High-intensity statin therapy alters the progressive nature of diabetic coronary atherosclerosis, yielding regression of disease in diabetic and nondiabetic patients. © 2014 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.

  10. Different methods to alter surface morphology of high aspect ratio structures

    Science.gov (United States)

    Leber, M.; Shandhi, M. M. H.; Hogan, A.; Solzbacher, F.; Bhandari, R.; Negi, S.

    2016-03-01

    In various applications such as neural prostheses or solar cells, there is a need to alter the surface morphology of high aspect ratio structures so that the real surface area is greater than geometrical area. The change in surface morphology enhances the devices functionality. One of the applications of altering the surface morphology is of neural implants such as the Utah electrode array (UEA) that communicate with single neurons by charge injection induced stimulation or by recording electrical neural signals. For high selectivity between single cells of the nervous system, the electrode surface area is required to be as small as possible, while the impedance is required to be as low as possible for good signal to noise ratios (SNR) during neural recording. For stimulation, high charge injection and charge transfer capacities of the electrodes are required, which increase with the electrode surface. Traditionally, researchers have worked with either increasing the roughness of the existing metallization (platinum grey, black) or other materials such as Iridium Oxide and PEDOT. All of these previously investigated methods lead to more complicated metal deposition processes that are difficult to control and often have a critical impact on the mechanical properties of the metal films. Therefore, a modification of the surface underneath the electrode's coating will increase its surface area while maintaining the standard and well controlled metal deposition process. In this work, the surfaces of the silicon micro-needles were engineered by creating a defined microstructure on the electrodes surface using several methods such as laser ablation, focused ion beam, sputter etching, reactive ion etching (RIE) and deep reactive ion etching (DRIE). The surface modification processes were optimized for the high aspect ratio silicon structures of the UEA. The increase in real surface area while maintaining the geometrical surface area was verified using scanning electron

  11. Quantifying braided river morphodynamics through a sequence of high-flow events

    Science.gov (United States)

    Williams, R. D.; Brasington, J.; Vericat, D.; Hicks, D. M.

    2012-04-01

    Quantifying braided river morphology and morphological change is a key task for understanding braided river behaviour. In the last decade, developments in geomatics technologies and associated data processing toolboxes have transformed the potential for producing precise, reach-scale topographic datasets. Moreover, since fast data acquisition rates enable surveys to be undertaken at frequencies that are commensurate with individual flood events, it is now possible to map morphological change for sequences of high-flow events over considerable spatial extents. The application of high-resolution remote sensing technologies to monitor braided river dynamics thus has the potential to provide considerable insight into the relationships between forcing discharges, sediment transport and morphological evolution. In this paper we present a set of Digital Elevation Models (DEMs) that have been produced by monitoring the evolution of a 2.5 x 0.7 km braided study area of the Rees River, New Zealand, through a sequence of ten high-flow events over an eight-month period. We then use the morphological approach to produce a sediment budget for the study area. The morphological evolution of the Rees River braided study area was monitored after each storm event using a combination of two remote sensing methodologies. First, dry areas of the braidplain were surveyed using a Terrestrial Laser Scanner (TLS) mounted on an Argo Amphibious All Terrain Vehicle. Second, since the TLS was not water penetrating, bathymetry was mapped using an empirically calibrated optical method, based on non-metric vertical aerial photos acquired from a helicopter and an acoustic depth survey along primary anabranches. The resulting data were fused together to produce high quality DEMs, with sub-cm and sub-decimetre vertical standard deviations of error for the TLS and optical-empirical bathymetric components respectively. The resulting set of DEMs enabled the quantification of morphological change through

  12. Linking River Management-Induced Perturbations of Hydrologic and Sediment Regimes to Geomorphic Processes Along a Highly-Dynamic Gravel-Bed River: Snake River, WY.

    Science.gov (United States)

    Leonard, C.; Legleiter, C. J.

    2015-12-01

    Encroachment of human development onto river floodplains creates a need to stabilize rivers and provide flood protection. Structural interventions, such as levees, often perturb hydrologic and sediment regimes and thus can initiate morphological responses. An understanding of how human activities affect river morphodynamics and trigger channel change is needed to anticipate future river responses and facilitate effective restoration. This study examines approximately 66 km of the Snake River, WY, USA, and links sediment transport processes to channel form and behavior by developing a morphological sediment budget that spans both a natural, unconfined reach and a reach confined by artificial levees. Sediment transport rates are inferred from the morphological sediment budget and a bed mobility study is used to estimate entrainment thresholds that allow us to link the hydrological regime during the sediment budget period to the observed channel changes. Results indicate that lateral constriction by levees triggers a positive feedback mechanism by incising the bed, focusing flow energy, thus increasing transport capacity, and leading to armoring of the bed. In other systems, armoring promotes widening of the channel but in this case levees prevent widening and the channel instead migrates across the braidplain rapidly, producing further erosion of bars and vegetated islands that is expressed as negative net volumetric changes and increased sediment transport rates. Furthermore, decreased slopes and reduced discharges due to dam regulation in the upstream unconfined reach cause gravel sheets to stall on bars and in other areas of storage, creating a spatial discontinuity in sediment conveyance downstream, and thus contributing to the sediment deficit within the leveed reach.

  13. Suspended sediment in a high-Arctic river: An appraisal of flux estimation methods.

    Science.gov (United States)

    Ladegaard-Pedersen, Pernille; Sigsgaard, Charlotte; Kroon, Aart; Abermann, Jakob; Skov, Kirstine; Elberling, Bo

    2017-02-15

    Quantifying fluxes of water, sediment and dissolved compounds through Arctic rivers is important for linking the glacial, terrestrial and marine ecosystems and to quantify the impact of a warming climate. The quantification of fluxes is not trivial. This study uses a 8-years data set (2005-2012) of daily measurements from the high-Artic Zackenberg River in Northeast Greenland to estimate annual suspended sediment fluxes based on four commonly used methods: M1) is the discharge weighted mean and uses direct measurements, while M2-M4) are one uncorrected and two bias corrected rating curves extrapolating a continuous concentration trace from measured values. All methods are tested on complete and reduced datasets. The average annual runoff in the period 2005-2012 was 190±25mio·m(3)y(-1). The different estimation methods gave a range of average annual suspended sediment fluxes between 43,000±10,000ty(-1) and 61,000±16,000ty(-1). Extreme events with high discharges had a mean duration of 1day. The average suspended sediment flux during extreme events was 17,000±5000ty(-1), which constitutes a year-to-year variation of 20-37% of the total annual flux. The most accurate sampling strategy was bi-daily sampling together with a sampling frequency of 2h during extreme events. The most consistent estimation method was an uncorrected rating curve of bi-daily measurements (M2), combined with a linear interpolation of extreme event fluxes. Sampling can be reduced to every fourth day, with both method-agreements and accuracies agreements were Arctic Zackenberg River, unless sampling was done bi-daily, every day and events sampled high-frequently.

  14. High glucose concentrations alter the biomineralization process in human osteoblastic cells.

    Science.gov (United States)

    García-Hernández, A; Arzate, H; Gil-Chavarría, I; Rojo, R; Moreno-Fierros, L

    2012-01-01

    Diabetes mellitus (DM) may alter bone remodeling, as osteopenia and osteoporosis are among the complications. Moreover, DM increases the risk and severity of chronic inflammatory periodontal disease, in which bone resorption occurs. Broad evidence suggests that chronic inflammation can contribute to the development of DM and its complications. Hyperglycemia is a hallmark of DM that may contribute to sustained inflammation by increasing proinflammatory cytokines, which are known to cause insulin resistance, via toll-like receptor (TLR)-4-mediated mechanisms. However, the mechanisms by which bone-related complications develop in DM are still unknown. Studies done on the effect of high glucose concentrations on osteoblast functions are contradictory because some suggest increases (although others suggest reductions) in the biomineralization process. Therefore, we evaluated the effect of high glucose levels on biomineralization and inflammation markers in a human osteoblastic cell line. Cells were treated with either physiological 5.5 mM or increasing concentrations of glucose up to 24 mM, and we determined the following: i) the quantity and quality of calcium-deposit crystals in culture and ii) the expression of the following: a) proteins associated with the process of biomineralization, b) the receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG), c) cytokines IL1, IL6, IL8, IL10, MCP-1 and TNF alpha, and d) TLR-2, -3, -4 and -9. Our results show that high glucose concentrations (12 mM and particularly 24 mM) alter the biomineralization process in osteoblastic cells and provoke the following: i) a rise in mineralization, ii) an increase in the mRNA expression of RANKL and a decrease of OPG, iii) an increase in the mRNA expression of osteocalcin, bone sialoprotein and the transcription factor Runx2, iv) a diminished quality of the mineral, and v) an increase in the expression of IL1beta, IL6, IL8, MCP-1 and IL10 mRNAs. In addition we

  15. High-resolution (spatial and temporal) Hydrodynamic Modeling in the Lower Mississippi River Delta

    Science.gov (United States)

    Karadogan, E.; Danchuk, S.; Berger, C.; Brown, G.; Willson, C.

    2007-12-01

    The lower Mississippi River is a highly engineered system existing in one of the world's largest deltas. This system is subject to a variety of spatial and temporal forcings due to its large watershed (drains about 41% of the continental U.S.) and from the Gulf of Mexico. Future perturbations on this system are anticipated due to the impacts of global climate change (e.g., rising eustatic sea level, changes in weather patterns) and from proposed modifications to the system such as diversion structures aimed at providing freshwater nutrients and sediments to the rapidly degrading coastal wetlands. Numerical modeling will play a large role in improving our understanding and management of the system and the ability to properly design future structural features. These models will need to have the necessary spatial and temporal resolution to account for the many important processes in the river, the Gulf of Mexico, and in the wetland areas where small distributary channels will form and wetting/drying must be accounted for. This paper will investigate the ability of a 2D shallow water and sediment model to reproduce the complex distributary development associated with flow diversions into quiescent bays. A reach of the Lower Mississippi River from Point a la Hache to the Gulf of Mexico was used as a test domain to evaluate the performance and capabilities of the U.S. Army Corps of Engineers ADaptive Hydraulics (ADH) model. ADH is an unstructured finite element modeling system that includes unsaturated Richards' equations for groundwater, Navier Stokes for nonhydrostatic flow calculations, and Shallow Water equations. ADH conducts automated refinement and coarsening of the mesh based upon flow characteristics. In this case the 2D shallow water model is being used. It includes coupled flow and sedimentation. An unstructured mesh was developed for the study area which includes detailed bathymetry and topography from available survey data. The mesh is fine enough to capture

  16. High resolution measurements of dune movement in a scale model of the River Oder

    Science.gov (United States)

    Hüsener, Thorsten; Henning, Martin

    2010-05-01

    The paper presents the analysis of three dimensional river bed topographies of high spatial and temporal resolution, obtained from scale model experiments with movable bed. The use of a stereo photogrammetric system allowed for measuring the submerged river bed during the laboratory experiments. The system is based on three synchronized cameras and a bar code system for orientation and can be used in both dry and wet conditions. For bed surface elevation measurements, a grid is projected onto the channel bed, defining the bed surface via slide projection. When applied to subaqueous problems, the system provides reliable data and insight in the distribution and migration of bed forms and the impact of steady and unsteady discharges on bed topography. The presented data has been obtained from a hydraulic scale model with moveable bed, concerning an 8km long reach of the River Oder at the German-Polish border. The model has been set up in order to investigate the influence of river training measures on accessible water depths and on the development of river bed forms. To determine the movement of the dunes, a 3 x 3 m² area of the model, representing 90,000 m² in field scale, has been recorded over a time of 11 h, providing 4000 topographic data sets of about 10,000 data points each. To simulate nature like transport conditions, the natural bedload material was substituted by synthetic granules (polystyrene) with lesser density and coarser diameter. Due to the small density of polystyrene the dune migration was considerably faster than it would have been for the use of sand as bed load material. In theory, flow is often assumed to be steady and uniform. However, during sediment transport, bed topography changes continuously. The presented analysis of the data shows the wide spatial and temporal variety of occurring dunes and the correlation between dune dimen-sions and dune migration speed. Possible future analysis of the three-dimensional data will be discussed and

  17. Effect of high sedimentation rates on surface sediment dynamics and mangrove growth in the Porong River, Indonesia.

    Science.gov (United States)

    Sidik, Frida; Neil, David; Lovelock, Catherine E

    2016-06-15

    Large quantities of mud from the LUSI (Lumpur Sidoarjo) volcano in northeastern Java have been channeled to the sea causing high rates of sediment delivery to the mouth of the Porong River, which has a cover of natural and planted mangroves. This study investigated how the high rates of sediment delivery affected vertical accretion, surface elevation change and the growth of Avicennia sp., the dominant mangrove species in the region. During our observations in 2010-2011 (4-5years after the initial volcanic eruption), very high rates of sedimentation in the forests at the mouth of the river gave rise to high vertical accretion of over 10cmy(-1). The high sedimentation rates not only resulted in reduced growth of Avicennia sp. mangrove trees at the two study sites at the Porong River mouth, but also gave rise to high soil surface elevation gains.

  18. Elk herbivory alters small mammal assemblages in high-elevation drainages.

    Science.gov (United States)

    Parsons, Elliott W R; Maron, John L; Martin, Thomas E

    2013-03-01

    Heavy herbivory by ungulates can substantially alter habitat, but the indirect consequences of habitat modification for animal assemblages that rely on that habitat are not well studied. This is a particularly important topic given that climate change can alter plant-herbivore interactions. We explored short-term responses of small mammal communities to recent exclusion of Rocky Mountain elk (Cervus elaphus) in high-elevation riparian drainages in northern Arizona, where elk impacts on vegetation have increased over the past quarter century associated with climate change. We used 10-ha elk exclosures paired with unfenced control drainages to examine how browsing influenced the habitat use, relative abundance, richness and diversity of a small mammal assemblage. We found that the small mammal assemblage changed significantly after 5 years of elk exclusion. Relative abundance of voles (Microtus mexicanus) increased in exclosure drainages, likely due to an increase in habitat quality. The relative abundances of woodrats (Neotoma neomexicana) and two species of mice (Peromyscus maniculatus and P. boylii) decreased in the controls, while remaining stable in exclosures. The decline of mice in control drainages was likely due to the decline in shrub cover that they use. Thus, elk exclusion may have maintained or improved habitat for mice inside the exclosures while habitat quality and mouse abundance both declined outside the fences. Finally, small mammal species richness increased in the exclosures relative to the controls while species diversity showed no significant trends. Together, our results show that relaxation of heavy herbivore pressure by a widespread native ungulate can lead to rapid changes in small mammal assemblages. Moreover, exclusion of large herbivores can yield rapid responses by vegetation that may enhance or maintain habitat quality for small mammal populations. © 2012 The Authors. Journal of Animal Ecology © 2012 British Ecological Society.

  19. High salt intake in pregnancy alters maturation of glomeruli in the rat offspring

    Directory of Open Access Journals (Sweden)

    Nadezda Koleganova

    2012-06-01

    Full Text Available There is currently discussion on the optimal salt intake and uncertainty whether both high and low salt intake is associated with adverse effects. One aspect has so far not be considered, i.e. the potential impact of salt intake during pregnancy on kidney function and blood pressure in the offspring. Faulty fetal programming, amongst others by high or low salt intake, leads to alterations in kidney morphology and albuminuria in the offspring. A low number of glomeruli is known to cause high blood pressure later in life. It was the purpose of the present study to clarify whether very high (or low salt intakes in pregnancy affect kidney development in the offspring. Sprague-Dawley rats were fed normal (0.15%, medium (1.3%, or high (8.0% salt diet during pregnancy and weaning. The number of glomeruli (mature, immature, and S-shape bodies was assessed at 1 week postnatally. The expression of proteins of interest was assessed (by western blotting at 1 week postnatally and at term. There was no difference between the groups with respect to litter size, birth weight, and placenta size. At age 1 week the number of S-shaped bodies was significantly lower (405±308 and the number of mature glomeruli (818±405 and layers of developing glomeruli (7.1±0.6 was significantly higher in the offspring of mothers on high-salt compared to the medium or low salt groups (1044±490, 460±304, and 5.9±0.9 respectively. As a net result the total number of glomeruli was significantly lower in the offspring of mothers on high-salt (9476±1264 compared to the medium or low salt groups (11175±1920. At 1 week of age in the offspring of mothers on high salt the glomeruli were bigger compared to lower salt intake. The expression of Pax-2 (54±23% vs. 100±28% and FGF-2 (72±33% vs. 100±30% was significantly lower in the offspring of mothers on high-salt consistent with their causative role. We conclude that high maternal salt intake during pregnancy accelerates maturation

  20. Estimates of Climate Change Impact on River Discharge in Japan Based on a Super-High-Resolution Climate Model

    Directory of Open Access Journals (Sweden)

    Yoshinobu Sato

    2012-01-01

    Full Text Available The impact of climate change on river discharge was assessed by hydrological simulations for several major river basins in Japan using the latest version of a super-high-resolution atmospheric general circulation model (AGCM with a horizontal resolution of about 20 km. Projections were made using two different datasets, one representing the present climate (1980 - 1999 and the other representing the end of the 21st century (2080 - 2099 assuming the SRES A1B scenario. River discharge was estimated by a distributed hydrological model calibrated against observed river discharge in advance. The results showed that even if the amount of precipitation does not change much in the future, river discharge will change significantly because of the increase in rainfall, decrease in snowmelt, and increase in evapotranspiration with higher air temperature. The impact of climate change on river discharge will be more significant in the northern part of Japan, especially in the Tohoku and Hokuriku regions. In these regions, the monthly average river discharge at the end of the 21st century was projected to be more than 200% higher in February and approximately 50 - 60% lower in May compared with the present flow. These results imply that the increase in air temperature has important consequences for the hydrological cycle, particularly in regions where the water supply is currently dominated by snowmelt.

  1. Water-level changes in the High Plains aquifer, Republican River Basin in Colorado, Kansas, and Nebraska, 2002 to 2015

    Science.gov (United States)

    McGuire, V.L.

    2016-12-29

    The High Plains aquifer underlies 111.8 million acres (about 175,000 square miles) in parts of eight States—Colorado, Kansas, Nebraska, New Mexico, Oklahoma, South Dakota, Texas, and Wyoming. More than 95 percent of the water withdrawn from the High Plains aquifer is used for irrigation. Water-level declines began in parts of the High Plains aquifer soon after the beginning of substantial irrigation with groundwater in the aquifer area (about 1950). The Republican River Basin is 15.9 million acres (about 25,000 square miles) and is located in northeast Colorado, northern Kansas, and southwest Nebraska. The Republican River Basin overlies the High Plains aquifer for 87 percent of the basin area. Water-level declines had begun in parts of the High Plains aquifer within the Republican River Basin by 1964. In 2002, management practices were enacted in the Middle Republican Natural Resources District in Nebraska to comply with the Republican River Compact Final Settlement. The U.S. Geological Survey, in cooperation with the Middle Republican Natural Resources District, completed a study of water-level changes in the High Plains aquifer within the Republican River Basin from 2002 to 2015 to enable the Middle Republican Natural Resources District to assess the effect of the management practices, which were specified by the Republican River Compact Final Settlement. Water-level changes determined from this study are presented in this report.Water-level changes from 2002 to 2015 in the High Plains aquifer within the Republican River Basin, by well, ranged from a rise of 9.4 feet to a decline of 43.2 feet. The area-weighted, average water-level change from 2002 to 2015 in this part of the aquifer was a decline of 4.5 feet.

  2. Reconstruction of a high-resolution late holocene arctic paleoclimate record from Colville River delta sediments.

    Energy Technology Data Exchange (ETDEWEB)

    Schreiner, Kathryn Melissa; Lowry, Thomas Stephen

    2013-10-01

    This work was partially supported by the Sandia National Laboratories, Laboratory Directed Research and Development (LDRD) fellowship program in conjunction with Texas A&M University (TAMU). The research described herein is the work of Kathryn M. Schreiner (Katie) and her advisor, Thomas S. Bianchi and represents a concise description of Katies dissertation that was submitted to the TAMU Office of Graduate Studies in May 2013 in partial fulfillment of her doctorate of philosophy degree. High Arctic permafrost soils contain a massive amount of organic carbon, accounting for twice as much carbon as what is currently stored as carbon dioxide in the atmosphere. However, with current warming trends this sink is in danger of thawing and potentially releasing large amounts of carbon as both carbon dioxide and methane into the atmosphere. It is difficult to make predictions about the future of this sink without knowing how it has reacted to past temperature and climate changes. This project investigated long term, fine scale particulate organic carbon (POC) delivery by the high-Arctic Colville River into Simpsons Lagoon in the near-shore Beaufort Sea. Modern POC was determined to be a mixture of three sources (riverine soils, coastal erosion, and marine). Downcore POC measurements were performed in a core close to the Colville River output and a core close to intense coastal erosion. Inputs of the three major sources were found to vary throughout the last two millennia, and in the Colville River core covary significantly with Alaskan temperature reconstructions.

  3. Amazon river dolphins (Inia geoffrensis) use a high-frequency short-range biosonar.

    Science.gov (United States)

    Ladegaard, Michael; Jensen, Frants Havmand; de Freitas, Mafalda; Ferreira da Silva, Vera Maria; Madsen, Peter Teglberg

    2015-10-01

    Toothed whales produce echolocation clicks with source parameters related to body size; however, it may be equally important to consider the influence of habitat, as suggested by studies on echolocating bats. A few toothed whale species have fully adapted to river systems, where sonar operation is likely to result in higher clutter and reverberation levels than those experienced by most toothed whales at sea because of the shallow water and dense vegetation. To test the hypothesis that habitat shapes the evolution of toothed whale biosonar parameters by promoting simpler auditory scenes to interpret in acoustically complex habitats, echolocation clicks of wild Amazon river dolphins were recorded using a vertical seven-hydrophone array. We identified 404 on-axis biosonar clicks having a mean SLpp of 190.3 ± 6.1 dB re. 1 µPa, mean SLEFD of 132.1 ± 6.0 dB re. 1 µPa(2)s, mean Fc of 101.2 ± 10.5 kHz, mean BWRMS of 29.3 ± 4.3 kHz and mean ICI of 35.1 ± 17.9 ms. Piston fit modelling resulted in an estimated half-power beamwidth of 10.2 deg (95% CI: 9.6-10.5 deg) and directivity index of 25.2 dB (95% CI: 24.9-25.7 dB). These results support the hypothesis that river-dwelling toothed whales operate their biosonars at lower amplitude and higher sampling rates than similar-sized marine species without sacrificing high directivity, in order to provide high update rates in acoustically complex habitats and simplify auditory scenes through reduced clutter and reverberation levels. We conclude that habitat, along with body size, is an important evolutionary driver of source parameters in toothed whale biosonars.

  4. Effects of fault-controlled CO2 alteration on mineralogical and geomechanical properties of reservoir and seal rocks, Crystal Geyser, Green River, Utah

    Science.gov (United States)

    Major, J. R.; Eichhubl, P.; Urquhart, A.; Dewers, T. A.

    2012-12-01

    An understanding of the coupled chemical and mechanical properties of reservoir and seal units undergoing CO2 injection is critical for modeling reservoir behavior in response to the introduction of CO2. The implementation of CO2 sequestration as a mitigation strategy for climate change requires extensive risk assessment that relies heavily on computer models of subsurface reservoirs. Numerical models are fundamentally limited by the quality and validity of their input parameters. Existing models generally lack constraints on diagenesis, failing to account for the coupled geochemical or geomechanical processes that affect reservoir and seal unit properties during and after CO2 injection. For example, carbonate dissolution or precipitation after injection of CO2 into subsurface brines may significantly alter the geomechanical properties of reservoir and seal units and thus lead to solution-enhancement or self-sealing of fractures. Acidified brines may erode and breach sealing units. In addition, subcritical fracture growth enhanced by the presence of CO2 could ultimately compromise the integrity of sealing units, or enhance permeability and porosity of the reservoir itself. Such unknown responses to the introduction of CO2 can be addressed by laboratory and field-based observations and measurements. Studies of natural analogs like Crystal Geyser, Utah are thus a critical part of CO2 sequestration research. The Little Grand Wash and Salt Wash fault systems near Green River, Utah, host many fossil and active CO2 seeps, including Crystal Geyser, serving as a faulted anticline CO2 reservoir analog. The site has been extensively studied for sequestration and reservoir applications, but less attention has been paid to the diagenetic and geomechanical aspects of the fault zone. XRD analysis of reservoir and sealing rocks collected along transects across the Little Grand Wash Fault reveal mineralogical trends in the Summerville Fm (a siltstone seal unit) with calcite and

  5. High-intensity urban light installation dramatically alters nocturnal bird migration.

    Science.gov (United States)

    Van Doren, Benjamin M; Horton, Kyle G; Dokter, Adriaan M; Klinck, Holger; Elbin, Susan B; Farnsworth, Andrew

    2017-10-02

    Billions of nocturnally migrating birds move through increasingly photopolluted skies, relying on cues for navigation and orientation that artificial light at night (ALAN) can impair. However, no studies have quantified avian responses to powerful ground-based light sources in urban areas. We studied effects of ALAN on migrating birds by monitoring the beams of the National September 11 Memorial & Museum's "Tribute in Light" in New York, quantifying behavioral responses with radar and acoustic sensors and modeling disorientation and attraction with simulations. This single light source induced significant behavioral alterations in birds, even in good visibility conditions, in this heavily photopolluted environment, and to altitudes up to 4 km. We estimate that the installation influenced ≈1.1 million birds during our study period of 7 d over 7 y. When the installation was illuminated, birds aggregated in high densities, decreased flight speeds, followed circular flight paths, and vocalized frequently. Simulations revealed a high probability of disorientation and subsequent attraction for nearby birds, and bird densities near the installation exceeded magnitudes 20 times greater than surrounding baseline densities during each year's observations. However, behavioral disruptions disappeared when lights were extinguished, suggesting that selective removal of light during nights with substantial bird migration is a viable strategy for minimizing potentially fatal interactions among ALAN, structures, and birds. Our results also highlight the value of additional studies describing behavioral patterns of nocturnally migrating birds in powerful lights in urban areas as well as conservation implications for such lighting installations.

  6. Chemistry of thermally altered high volatile bituminous coals from southern Indiana

    Science.gov (United States)

    Walker, R.; Mastalerz, Maria; Brassell, S.; Elswick, E.; Hower, J.C.; Schimmelmann, A.

    2007-01-01

    The optical properties and chemical characteristics of two thermally altered Pennsylvanian high volatile bituminous coals, the non-coking Danville Coal Member (Ro = 0.55%) and the coking Lower Block Coal Member (Ro = 0.56%) were investigated with the purpose of understanding differences in their coking behavior. Samples of the coals were heated to temperatures of 275????C, 325????C, 375????C and 425????C, with heating times of up to one hour. Vitrinite reflectance (Ro%) rises with temperature in both coals, with the Lower Block coal exhibiting higher reflectance at 375????C and 425????C compared to the Danville coal. Petrographic changes include the concomitant disappearance of liptinites and development of vesicles in vitrinites in both coals, although neither coal developed anisotropic coke texture. At 375????C, the Lower Block coal exhibits a higher aromatic ratio, higher reflectance, higher carbon content, and lower oxygen content, all of which indicate a greater degree of aromatization at this temperature. The Lower Block coal maintains a higher CH2/CH3 ratio than the Danville coal throughout the heating experiment, indicating that the long-chain unbranched aliphatics contained in Lower Block coal liptinites are more resistant to decomposition. As the Lower Block coal contains significant amounts of liptinite (23.6%), the contribution of aliphatics from these liptinites appears to be the primary cause of its large plastic range and high fluidity. ?? 2006 Elsevier B.V. All rights reserved.

  7. Use of a backpack alters gait initiation of high school students.

    Science.gov (United States)

    Vieira, Marcus Fraga; Lehnen, Georgia Cristina; Noll, Matias; Rodrigues, Fábio Barbosa; de Avelar, Ivan Silveira; da Costa, Paula Hentschel Lobo

    2016-06-01

    We assessed how backpack carriage influences the gait initiation (GI) process in high school students, who extensively use backpacks. GI involves different dynamics from gait itself, while the excessive use of backpacks can result in adverse effects. 117 high school students were evaluated in three experimental conditions: no backpack (NB), bilateral backpack (BB), and unilateral backpack (UB). Two force plates were used to acquire ground reaction forces (GRFs) and moments for each foot separately. Center of pressure (COP) scalar variables were extracted, and statistical parametric mapping analysis was performed over the entire COP/GRFs time series. GI anticipatory postural adjustments (APAs) were reduced and were faster in backpack conditions; medial-lateral COP excursion was smaller in this phase. The uneven distribution of the extra load in the UB condition led to a larger medial-lateral COP shift in the support-foot unloading phase, with a corresponding vertical GRF change that suggests a more pronounced unloading swing foot/loading support foot mechanism. The anterior-posterior GRFs were altered, but the COP was not. A possible explanation for these results may be the forward trunk lean and the center of mass proximity of the base of support boundary, which induced smaller and faster APA, increased swing foot/support foot weight transfer, and increased load transfer to the first step.

  8. Maternal High Fat Diet Alters Skeletal Muscle Mitochondrial Catalytic Activity in Adult Male Rat Offspring

    Science.gov (United States)

    Pileggi, Chantal A.; Hedges, Christopher P.; Segovia, Stephanie A.; Markworth, James F.; Durainayagam, Brenan R.; Gray, Clint; Zhang, Xiaoyuan D.; Barnett, Matthew P. G.; Vickers, Mark H.; Hickey, Anthony J. R.; Reynolds, Clare M.; Cameron-Smith, David

    2016-01-01

    A maternal high-fat (HF) diet during pregnancy can lead to metabolic compromise, such as insulin resistance in adult offspring. Skeletal muscle mitochondrial dysfunction is one mechanism contributing to metabolic impairments in insulin resistant states. Therefore, the present study aimed to investigate whether mitochondrial dysfunction is evident in metabolically compromised offspring born to HF-fed dams. Sprague-Dawley dams were randomly assigned to receive a purified control diet (CD; 10% kcal from fat) or a high fat diet (HFD; 45% kcal from fat) for 10 days prior to mating, throughout pregnancy and during lactation. From weaning, all male offspring received a standard chow diet and soleus muscle was collected at day 150. Expression of the mitochondrial transcription factors nuclear respiratory factor-1 (NRF1) and mitochondrial transcription factor A (mtTFA) were downregulated in HF offspring. Furthermore, genes encoding the mitochondrial electron transport system (ETS) respiratory complex subunits were suppressed in HF offspring. Moreover, protein expression of the complex I subunit, NDUFB8, was downregulated in HF offspring (36%), which was paralleled by decreased maximal catalytic linked activity of complex I and III (40%). Together, these results indicate that exposure to a maternal HF diet during development may elicit lifelong mitochondrial alterations in offspring skeletal muscle. PMID:27917127

  9. Niacin alters the ruminal microbial composition of cattle under high-concentrate condition

    Directory of Open Access Journals (Sweden)

    Dan Luo

    2017-06-01

    Full Text Available To understand the effects of niacin on the ruminal microbial ecology of cattle under high-concentrate diet condition, Illumina MiSeq sequencing technology was used. Three cattle with rumen cannula were used in a 3 × 3 Latin-square design trial. Three diets were fed to these cattle during 3 periods for 3 days, respectively: high-forage diet (HF; forage-to-concentrate ratio = 80:20, high-concentrate diet (HC; forage-to-concentrate ratio = 20:80, and HC supplemented with 800 mg/kg niacin (HCN. Ruminal pH was measured before feeding and every 2 h after initiating feeding. Ruminal fluid was sampled at the end of each period for microbial DNA extraction. Overall, our findings revealed that subacute ruminal acidosis (SARA was induced and the α-diversity of ruminal bacterial community decreased in the cattle of HC group. Adding niacin in HC could relieve the symptoms of SARA in the cattle but the ruminal pH value and the Shannon index of ruminal bacterial community of HCN group were still lower than those of HF group. Whatever the diet was, the ruminal bacterial community of cattle was dominated by Bacteroidetes, Firmicutes and Proteobacteria. High-concentrate diet significantly increased the abundance of Prevotella, and decreased the abundance of Paraprevotella, Sporobacter, Ruminococcus and Treponema than HF. Compared with HC, HCN had a trend to decrease the percentage of Prevotella, and to increase the abundance of Succiniclasticum, Acetivibrio and Treponema. Increasing concentrate ratio could decrease ruminal pH value, and change the ruminal microbial composition. Adding niacin in HC could increase the ruminal pH value, alter the ruminal microbial composition.

  10. High temperature vitrification of surrogate Savannah River Site (SRS) mixed waste materials

    Energy Technology Data Exchange (ETDEWEB)

    Applewhite-Ramsey, A.; Schumacher, R.F.; Spatz, T.L. [Westinghouse Savannah River Co., Aiken, SC (United States); Newsom, R.A.; Circeo, L.J. [Georgia Inst. of Technology, Atlanta, GA (United States); Danjaji, M.B. [Clark Atlanta Univ., Atlanta, GA (United States)

    1995-11-01

    The Savannah River Technology Center (SRTC) has been funded through the DOE Office of Technology Development (DOE-OTD) to investigate high-temperature vitrification technologies for the treatment of diverse low-level and mixed wastes. High temperature vitrification is a likely candidate for processing heterogeneous solid wastes containing low levels of activity. Many SRS wastes fit into this category. Plasma torch technology is one high temperature vitrification method. A trial demonstration of plasma torch processing is being performed at the Georgia Institute of Technology on surrogate SRS wastes. This effort is in cooperation with the Engineering Research and Development Association of Georgia Universities (ERDA) program. The results of phase 1 of these plasma torch trials will be presented.

  11. High-level waste processing at the Savannah River Site: An update

    Energy Technology Data Exchange (ETDEWEB)

    Marra, J.E.; Bennett, W.M.; Elder, H.H.; Lee, E.D.; Marra, S.L.; Rutland, P.L.

    1997-09-01

    The Defense Waste Processing Facility (DWPF) at the Savannah River Site (SRS) in Aiken, SC mg began immobilizing high-level radioactive waste in borosilicate glass in 1996. Currently, the radioactive glass is being produced as a ``sludge-only`` composition by combining washed high-level waste sludge with glass frit. The glass is poured in stainless steel canisters which will eventually be disposed of in a permanent, geological repository. To date, DWPF has produced about 100 canisters of vitrified waste. Future processing operations will, be based on a ``coupled`` feed of washed high-level waste sludge, precipitated cesium, and glass frit. This paper provides an update of the processing activities completed to date, operational/flowsheet problems encountered, and programs underway to increase production rates.

  12. Isotopic and physical evidence for persistently high eruption temperatures for Yellowstone-Snake River Plain rhyolites

    Science.gov (United States)

    Loewen, M.; Bindeman, I. N.; Melnik, O. E.

    2015-12-01

    Low crystallinity rhyolite lavas and tuffs from the Yellowstone-Snake River plain system were long-thought to erupt at high 800-900 °C temperatures with evidence derived from experimental work and geothermometry (e.g., QUILF, Ti-in-quartz). Despite this evidence, newer experimental phase equilibria studies as well as a reformulation of zircon saturation temperatures support lower temperature magma eruption conditions. Here we present two independent lines of evidence for 850 °C and greater temperatures. We present high precision oxygen isotope thermometry for coexisting quartz, glass, pyroxene, and magnetite in order make temperature estimates independent of phase equilibria. For all analyzed Snake River Plain-Yellowstone rhyolites, we determine 800-1100 °C temperatures for clinopyroxene and 850-1100 °C temperatures for magnetite. Extremely slow oxygen diffusion in pyroxene will preserve oxygen isotope crystal composition for millions of years stored at magmatic temperatures. Interestingly, oxygen in magnetite will reequilibrate in ice caps or prexisiting topography did not otherwise restrict flow. Using these results and simple conductive cooling models, we show that flows erupted at >800 °C and probably ~850 °C in order to be emplaced before cooling below the melt-glass transition and forming a more dome-like and lobate morphology.

  13. Probability modeling of high flow extremes in Yingluoxia watershed, the upper reaches of Heihe River basin

    Science.gov (United States)

    Li, Zhanling; Li, Zhanjie; Li, Chengcheng

    2014-05-01

    Probability modeling of hydrological extremes is one of the major research areas in hydrological science. Most basins in humid and semi-humid south and east of China are concerned for probability modeling analysis of high flow extremes. While, for the inland river basin which occupies about 35% of the country area, there is a limited presence of such studies partly due to the limited data availability and a relatively low mean annual flow. The objective of this study is to carry out probability modeling of high flow extremes in the upper reach of Heihe River basin, the second largest inland river basin in China, by using the peak over threshold (POT) method and Generalized Pareto Distribution (GPD), in which the selection of threshold and inherent assumptions for POT series are elaborated in details. For comparison, other widely used probability distributions including generalized extreme value (GEV), Lognormal, Log-logistic and Gamma are employed as well. Maximum likelihood estimate is used for parameter estimations. Daily flow data at Yingluoxia station from 1978 to 2008 are used. Results show that, synthesizing the approaches of mean excess plot, stability features of model parameters, return level plot and the inherent independence assumption of POT series, an optimum threshold of 340m3/s is finally determined for high flow extremes in Yingluoxia watershed. The resulting POT series is proved to be stationary and independent based on Mann-Kendall test, Pettitt test and autocorrelation test. In terms of Kolmogorov-Smirnov test, Anderson-Darling test and several graphical diagnostics such as quantile and cumulative density function plots, GPD provides the best fit to high flow extremes in the study area. The estimated high flows for long return periods demonstrate that, as the return period increasing, the return level estimates are probably more uncertain. The frequency of high flow extremes exhibits a very slight but not significant decreasing trend from 1978 to

  14. High Resolution 3-D Finite-Volume Coastal Ocean Modeling in Lower Campbell River and Discovery Passage, British Columbia, Canada

    Directory of Open Access Journals (Sweden)

    Yuehua Lin

    2014-03-01

    Full Text Available The 3-D unstructured-grid, Finite-Volume Coastal Ocean Model (FVCOM was used to simulate the flows in Discovery Passage including the adjoining Lower Campbell River, British Columbia, Canada. Challenges in the studies include the strong tidal currents (e.g., up to 7.8 m/s in Seymour Narrows and tailrace discharges, small-scale topographic features and steep bottom slopes, and stratification affected by the Campbell River freshwater discharges. Two applications of high resolution 3-D FVCOM modeling were conducted. One is for the Lower Campbell River extending upstream as far as the John Hart Hydroelectric dam. The horizontal resolution varies from 0.27 m to 32 m in the unstructured triangular mesh to resolve the tailrace flow. The bottom elevation decreases ~14 m within the distance of ~1.4 km along the river. This pioneering FVCOM river modeling demonstrated a very good performance in simulating the river flow structures. The second application is to compute ocean currents immediately above the seabed along the present underwater electrical cable crossing routes across Discovery Passage. Higher resolution was used near the bottom with inter-layer spacing ranging from 0.125 to 0.0005 of total water depth. The model behaves very well in simulating the strong tidal currents in the area at high resolution in both the horizontal and vertical. One year maximum near bottom tidal current along the routes was then analyzed using the model results.

  15. Native fish sanctuaries of the lower Colorado River: Cibola High Levee Pond, Desert Pupfish Pond

    Science.gov (United States)

    Mueller, G.

    2005-01-01

    Historically, the Colorado River was one of the most formidable rivers in the world. Each spring, melting snow from the mountains scoured the desert landscape moving millions of tons of sediment to the sea. The Grand Canyon lays testament to its erosive nature. Summer heat would bring seasonal droughts, reducing the river to a trickle impacting humans, animals, and fish.

  16. Independent Assessment of the Savannah River Site High-Level Waste Salt Disposition Alternatives Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    J. T. Case (DOE-ID); M. L. Renfro (INEEL)

    1998-12-01

    This report presents the results of the Independent Project Evaluation (IPE) Team assessment of the Westinghouse Savannah River Company High-Level Waste Salt Disposition Systems Engineering (SE) Team's deliberations, evaluations, and selections. The Westinghouse Savannah River Company concluded in early 1998 that production goals and safety requirements for processing SRS HLW salt to remove Cs-137 could not be met in the existing In-Tank Precipitation Facility as currently configured for precipitation of cesium tetraphenylborate. The SE Team was chartered to evaluate and recommend an alternative(s) for processing the existing HLW salt to remove Cs-137. To replace the In-Tank Precipitation process, the Savannah River Site HLW Salt Disposition SE Team downselected (October 1998) 140 candidate separation technologies to two alternatives: Small-Tank Tetraphenylborate (TPB) Precipitation (primary alternative) and Crystalline Silicotitanate (CST) Nonelutable Ion Exchange (backup alternative). The IPE Team, commissioned by the Department of Energy, concurs that both alternatives are technically feasible and should meet all salt disposition requirements. But the IPE Team judges that the SE Team's qualitative criteria and judgments used in their downselection to a primary and a backup alternative do not clearly discriminate between the two alternatives. To properly choose between Small-Tank TPB and CST Ion Exchange for the primary alternative, the IPE Team suggests the following path forward: Complete all essential R and D activities for both alternatives and formulate an appropriate set of quantitative decision criteria that will be rigorously applied at the end of the R and D activities. Concurrent conceptual design activities should be limited to common elements of the alternatives.

  17. Locomotor muscle fatigue does not alter oxygen uptake kinetics during high-intensity exercise

    Directory of Open Access Journals (Sweden)

    James Hopker

    2016-10-01

    Full Text Available The slow component (VO2sc that develops during high-intensity aerobic exercise is thought to be strongly associated with locomotor muscle fatigue. We sought to experimentally test this hypothesis by pre-fatiguing the locomotor muscles used during subsequent high-intensity cycling exercise. Over two separate visits, eight healthy male participants were asked to either perform a non-metabolically stressful 100 intermittent drop-jumps protocol (pre fatigue condition or rest for 33 minutes (control condition according to a random and counterbalanced order. Locomotor muscle fatigue was quantified with 6-second maximal sprints at a fixed pedaling cadence of 90 rev·min-1. Oxygen kinetics and other responses (heart rate, capillary blood lactate concentration and rating of perceived exertion, RPE were measured during two subsequent bouts of 6 min cycling exercise at 50% of the delta between the lactate threshold and VO2max determined during a preliminary incremental exercise test. All tests were performed on the same cycle ergometer. Despite significant locomotor muscle fatigue (P = 0.03, the VO2sc was not significantly different between the pre fatigue (464 ± 301 mL·min-1 and the control (556 ± 223 mL·min-1 condition (P = 0.50. Blood lactate response was not significantly different between conditions (P = 0.48 but RPE was significantly higher following the pre-fatiguing exercise protocol compared with the control condition (P < 0.01 suggesting higher muscle recruitment. These results demonstrate experimentally that locomotor muscle fatigue does not significantly alter the VO2 kinetic response to high intensity aerobic exercise, and challenge the hypothesis that the VO2sc is strongly associated with locomotor muscle fatigue.

  18. Local adaptation in Trinidadian guppies alters stream ecosystem structure at landscape scales despite high environmental variability

    Science.gov (United States)

    Simon, Troy N.; Bassar, Ronald D.; Binderup, Andrew J.; Flecker, Alex S.; Freeman, Mary C.; Gilliam, James F.; Marshall, Michael C.; Thomas, Steve A.; Travis, Joseph; Reznick, David N.; Pringle, Catherine M.

    2017-01-01

    While previous studies have shown that evolutionary divergence alters ecological processes in small-scale experiments, a major challenge is to assess whether such evolutionary effects are important in natural ecosystems at larger spatial scales. At the landscape scale, across eight streams in the Caroni drainage, we found that the presence of locally adapted populations of guppies (Poecilia reticulata) is associated with reduced algal biomass and increased invertebrate biomass, while the opposite trends were true in streams with experimentally introduced populations of non-locally adapted guppies. Exclusion experiments conducted in two separate reaches of a single stream showed that guppies with locally adapted phenotypes significantly reduced algae with no effect on invertebrates, while non-adapted guppies had no effect on algae but significantly reduced invertebrates. These divergent effects of phenotype on stream ecosystems are comparable in strength to the effects of abiotic factors (e.g., light) known to be important drivers of ecosystem condition. They also corroborate the results of previous experiments conducted in artificial streams. Our results demonstrate that local adaptation can produce phenotypes with significantly different effects in natural ecosystems at a landscape scale, within a tropical watershed, despite high variability in abiotic factors: five of the seven physical and chemical parameters measured across the eight study streams varied by more than one order of magnitude. Our findings suggest that ecosystem structure is, in part, an evolutionary product and not simply an ecological pattern.

  19. Functional alterations in neural substrates of geometric reasoning in adults with high-functioning autism.

    Directory of Open Access Journals (Sweden)

    Takashi Yamada

    Full Text Available Individuals with autism spectrum condition (ASC are known to excel in some perceptual cognitive tasks, but such developed functions have been often regarded as "islets of abilities" that do not significantly contribute to broader intellectual capacities. However, recent behavioral studies have reported that individuals with ASC have advantages for performing Raven's (Standard Progressive Matrices (RPM/RSPM, a standard neuropsychological test for general fluid intelligence, raising the possibility that ASC's cognitive strength can be utilized for more general purposes like novel problem solving. Here, the brain activity of 25 adults with high-functioning ASC and 26 matched normal controls (NC was measured using functional magnetic resonance imaging (fMRI to examine neural substrates of geometric reasoning during the engagement of a modified version of the RSPM test. Among the frontal and parietal brain regions involved in fluid intelligence, ASC showed larger activation in the left lateral occipitotemporal cortex (LOTC during an analytic condition with moderate difficulty than NC. Activation in the left LOTC and ventrolateral prefrontal cortex (VLPFC increased with task difficulty in NC, whereas such modulation of activity was absent in ASC. Furthermore, functional connectivity analysis revealed a significant reduction of activation coupling between the left inferior parietal cortex and the right anterior prefrontal cortex during both figural and analytic conditions in ASC. These results indicate altered pattern of functional specialization and integration in the neural system for geometric reasoning in ASC, which may explain its atypical cognitive pattern, including performance on the Raven's Matrices test.

  20. High temperatures alter physiological properties of pyramidal cells and inhibitory interneurons in hippocampus

    Directory of Open Access Journals (Sweden)

    Jennifer eKim

    2012-07-01

    Full Text Available Temperature has multiple effects on neurons, yet little is known about the effects of high temperature on the physiology of mammalian central neurons. Hyperthermia can influence behavior and cause febrile seizures. We studied the effects of acute hyperthermia on the immature hippocampus in vitro by recording from pyramidal neurons and inhibitory oriens-lacunosum moleculare (O-LM interneurons (identified by green fluorescent protein expression in the GIN mouse line. Warming to 41°C caused depolarization, spontaneous action potentials, reduced input resistance and membrane time constant, and increased spontaneous synaptic activity of most pyramidal cells and O-LM interneurons. Pyramidal neurons of area CA3 were more strongly excited by hyperthermia than those of area CA1. About 90% of O-LM interneurons in both CA1 and CA3 increased their firing rates at hyperthermic temperatures; interneurons in CA3 fired faster than those in CA1 on average. Blockade of fast synaptic transmission did not abolish the effect of hyperthermia on neuronal excitability. Our results suggest that hyperthermia increases hippocampal excitability, particularly in seizure-prone area CA3, by altering the intrinsic membrane properties of pyramidal cells and interneurons.

  1. High temperatures alter physiological properties of pyramidal cells and inhibitory interneurons in hippocampus.

    Science.gov (United States)

    Kim, Jennifer A; Connors, Barry W

    2012-01-01

    Temperature has multiple effects on neurons, yet little is known about the effects of high temperature on the physiology of mammalian central neurons. Hyperthermia can influence behavior and cause febrile seizures. We studied the effects of acute hyperthermia on the immature hippocampus in vitro by recording from pyramidal neurons and inhibitory oriens-lacunosum moleculare (O-LM) interneurons (identified by green fluorescent protein (GFP) expression in the GIN mouse line). Warming to 41°C caused depolarization, spontaneous action potentials, reduced input resistance and membrane time constant, and increased spontaneous synaptic activity of most pyramidal cells and O-LM interneurons. Pyramidal neurons of area CA3 were more strongly excited by hyperthermia than those of area CA1. About 90% of O-LM interneurons in both CA1 and CA3 increased their firing rates at hyperthermic temperatures; interneurons in CA3 fired faster than those in CA1 on average. Blockade of fast synaptic transmission did not abolish the effect of hyperthermia on neuronal excitability. Our results suggest that hyperthermia increases hippocampal excitability, particularly in seizure-prone area CA3, by altering the intrinsic membrane properties of pyramidal cells and interneurons.

  2. Altered slow wave activity in major depressive disorder with hypersomnia: a high density EEG pilot study.

    Science.gov (United States)

    Plante, David T; Landsness, Eric C; Peterson, Michael J; Goldstein, Michael R; Wanger, Tim; Guokas, Jeff J; Tononi, Giulio; Benca, Ruth M

    2012-03-31

    Hypersomnolence in major depressive disorder (MDD) plays an important role in the natural history of the disorder, but the basis of hypersomnia in MDD is poorly understood. Slow wave activity (SWA) has been associated with sleep homeostasis, as well as sleep restoration and maintenance, and may be altered in MDD. Therefore, we conducted a post-hoc study that utilized high density electroencephalography (hdEEG) to test the hypothesis that MDD subjects with hypersomnia (HYS+) would have decreased SWA relative to age- and sex-matched MDD subjects without hypersomnia (HYS-) and healthy controls (n=7 for each group). After correction for multiple comparisons using statistical non-parametric mapping, HYS+ subjects demonstrated significantly reduced parieto-occipital all-night SWA relative to HYS- subjects. Our results suggest hypersomnolence may be associated with topographic reductions in SWA in MDD. Further research using an adequately powered prospective design is indicated to confirm these findings. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  3. Benefits of prescribed flows for salmon smolt survival enhancement vary longitudinally in a highly managed river system

    Science.gov (United States)

    Courter, Ian; Garrison, Thomas; Kock, Tobias J.; Perry, Russell W.; Child, David; Hubble, Joel

    2016-01-01

    The influence of streamflow on survival of emigrating juvenile Pacific salmonids Oncorhynchus spp. (smolts) is a major concern for water managers throughout the northeast Pacific Rim. However, few studies have quantified flow effects on smolt survival, and available information does not indicate a consistent flow–survival relationship within the typical range of flows under management control. In the Yakima Basin, Washington, the potential effects of streamflow alterations on smolt survival have been debated for over 20 years. Using a series of controlled flow releases from upper basin reservoirs and radiotelemetry, we quantified the relationship between flow and yearling Chinook salmon smolt survival in the 208 km reach between Roza Dam and the Yakima River mouth. A multistate mark–recapture model accounted for weekly variation in flow conditions experienced by tagged fish in four discrete river segments. Smolt survival was significantly associated with streamflow in the Roza Reach [river kilometre (rkm) 208–189] and marginally associated with streamflow in the Sunnyside Reach (rkm 169–77). However, smolt survival was not significantly associated with flow in the Naches and Prosser Reaches (rkm 189–169 and rkm 77–3). This discrepancy indicates potential differences in underlying flow-related survival mechanisms, such as predation or passage impediments. Our results clarify trade-offs between flow augmentation for fisheries enhancement and other beneficial uses, and our study design provides a framework for resolving uncertainties about streamflow effects on migratory fish survival in other river systems. 

  4. Development of a high-resolution bathymetry dataset for the Columbia River through the Hanford Reach

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, Andre M.; Ward, Duane L.; Larson, Kyle B.; Lettrick, Joseph W.

    2010-10-08

    A bathymetric and topographic data collection and processing effort involving existing and newly collected data has been performed for the Columbia River through the Hanford Reach in central Washington State, extending 60-miles from the tailrace of Priest Rapids Dam (river mile 397) to near the vicinity of the Interstate 182 bridge just upstream of the Yakima River confluence (river mile 337). The contents of this report provide a description of the data collections, data inputs, processing methodology, and final data quality assessment used to develop a comprehensive and continuous merged 1m resolution bathymetric and topographic surface dataset for the Columbia River through the Hanford Reach.

  5. High Mortality of Nearctic River Otters on a Florida, USA Interstate Highway During an Extreme Drought

    Directory of Open Access Journals (Sweden)

    Kinlaw A.

    2004-10-01

    Full Text Available From 28 December 1999 until 15 July 2000, I recorded 15 river otters killed on a 16 km segment of Interstate highway in Polk County, Florida, USA. This included 9 during a 37 day period, the highest road mortality rate reported for North American otters. This compares to 22 otters killed on a south Florida road during a 2 year period. My sample of 15 killed on I-4 is a negatively biased count of the true number killed, due to the minimal sampling period and sampling method. Possible reasons for such a high number counted during such a short period are discussed, including the impact of a major drought, high traffic density, and possible dieldrin bioaccumulation.

  6. Sewage-derived nutrient dynamics in highly urbanized coastal rivers, western Japan

    Science.gov (United States)

    Onodera, S. I.; Saito, M.; Jin, G.; Taniguchi, M.

    2016-12-01

    Water pollution by domestic sewage is one of the critical environmental problems in the early stage of urbanization with significant growth of population. In case of Osaka metropolitan area in Japan, the pollution was significant until 1970s, while it has been improved by the development of sewage treatment systems. However, removal of nitrogen needs the advanced process therefore relatively large part of dissolved inorganic nitrogen (DIN) is usually discharged by treated sewage effluent. Besides, increase of sewage-derived pollutant loads through the combined sewage systems during rainfall events is recognized as a new problem in recent years. However, the impacts of sewage-derived loads on the water environment of river and coastal area have not been fully evaluated in previous studies. In the present research, we aimed to examine the dynamics of sewage-derived nutrients in highly urbanized coastal rivers. Study area is located on the coastal area of Osaka bay in Seto Inland Sea, western Japan. Treated sewage effluent is discharged from three sewage treatment plants (KH, SU and SA) to a river and channels. Water and sediment samples were collected and electric conductivity (EC), chlorophyll-a (Chl.-a) and dissolved oxygen concentration (DO) were measured from the discharging points to few kilometers offshore at 100-300 m intervals. Nutrients (nitrogen, phosphorus and silica), nitrogen and carbon contents and stable isotope ratios (δ15N and δ13C) of particulate organic matter (POM) and sediment, nitrogen and oxygen stable isotope ratios (δ15N and δ18O) in nitrate (NO3-) were measured. Nitrate-nitrogen (NO3-N) concentration were significantly high near the discharging point then it decreased to offshore suggesting that impact zone of sewage effluent is about 1 km from the discharging point. Significant NO3-N uptake by phytoplankton as well as dilution process were suggested in the area. However, the impact zone expanded more than twofold during the rainfall

  7. Fluvial sediment transport in a glacier-fed high-mountain river (Riffler Bach, Austrian Alps)

    Science.gov (United States)

    Morche, David; Weber, Martin; Faust, Matthias; Schuchardt, Anne; Baewert, Henning

    2017-04-01

    High-alpine environments are strongly affected by glacier retreat since the Little Ice Age (LIA). Due to ongoing climate change the hydrology of proglacial rivers is also influenced. It is expected that the growing proportions of snow melt and rainfall events will change runoff characteristics of proglacial rivers. Additionally, the importance of paraglacial sediment sources in recently deglaciating glacier forefields is increasing, while the role of glacial erosion is declining. Thus complex environmental conditions leading to a complex pattern of fluvial sediment transport in partly glaciated catchments of the European Alps. Under the umbrella of the joint PROSA-project the fluvial sediment transport of the river Riffler Bach (Kaunertal, Tyrol, Austria) was studied in 3 consecutive ablation seasons in order to quantify sediment yields. In June 2012 a probe for water level and an automatic water sampler (AWS) were installed at the outlet of the catchment (20km2). In order to calculate annual stage-discharge-relations by the rating-curve approach, discharge (Q) was repeatedly measured with current meters and by salt dilution. Concurrent to the discharge measurements bed load was collected using a portable Helley-Smith sampler. Bed load samples were weighted and sieved in the laboratory to gain annual bed load rating curves and grain size distributions. In total 564 (2012: 154, 2013: 209, 2014: 201) water samples were collected and subsequently filtered to quantify suspended sediment concentrations (SSC). Q-SSC-relations were calculated for single flood events due to the high variability of suspended sediment transport. The results show a high inter- and intra-annual variability of solid fluvial sediment transport, which can be explained by the characteristics of suspended sediment transport. Only 13 of 22 event-based Q-SSC-relations show causal dependency. In 2012, during a period with multiple pluvial-induced peak discharges most sediment was transported. On the

  8. Alteration of plasma glutamate and glutamine levels in children with high-functioning autism.

    Directory of Open Access Journals (Sweden)

    Chie Shimmura

    Full Text Available BACKGROUND: It has recently been hypothesized that hyperglutamatergia in the brain is involved in the pathophysiology of autism. However, there is no conclusive evidence of the validity of this hypothesis. As peripheral glutamate/glutamine levels have been reported to be correlated with those of the central nervous system, the authors examined whether the levels of 25 amino acids, including glutamate and glutamine, in the platelet-poor plasma of drug-naïve, male children with high-functioning autism (HFA would be altered compared with those of normal controls. METHODOLOGY/PRINCIPAL FINDINGS: Plasma levels of 25 amino acids in male children (N = 23 with HFA and normally developed healthy male controls (N = 22 were determined using high-performance liquid chromatography. Multiple testing was allowed for in the analyses. Compared with the normal control group, the HFA group had higher levels of plasma glutamate and lower levels of plasma glutamine. No significant group difference was found in the remaining 23 amino acids. The effect size (Cohen's d for glutamate and glutamine was large: 1.13 and 1.36, respectively. Using discriminant analysis with logistic regression, the two values of plasma glutamate and glutamine were shown to well-differentiate the HFA group from the control group; the rate of correct classification was 91%. CONCLUSIONS/SIGNIFICANCE: The present study suggests that plasma glutamate and glutamine levels can serve as a diagnostic tool for the early detection of autism, especially normal IQ autism. These findings indicate that glutamatergic abnormalities in the brain may be associated with the pathobiology of autism.

  9. Aquatic assemblages of the highly urbanized Santa Ana River Basin, California

    Science.gov (United States)

    Brown, L.R.; Burton, C.A.; Belitz, K.

    2005-01-01

    We assessed the structure of periphyton, benthic macroinvertebrate, and fish assemblages and their associations with environmental variables at 17 sites on streams of the highly urbanized Santa Ana River basin in Southern California. All assemblages exhibited strong differences between highly urbanized sites in the valley and the least-impacted sites at the transition between the valley and undeveloped mountains. Results within the urbanized area differed among taxa. Periphyton assemblages were dominated by diatoms (>75% of total taxa). Periphyton assemblages within the urbanized area were not associated with any of the measured environmental variables, suggesting that structure of urban periphyton assemblages might be highly dependent on colonization dynamics. The number of Ephemeroptera, Trichoptera, and Plecoptera (EPT) taxa included in macroinvertebrate assemblages ranged from 0 to 6 at urbanized sites. Benthic macroinvertebrate assemblages had significant correlations with several environmental variables within the urban area, suggesting that stream size and permanence were important determinants of distribution among the species able to survive conditions in urban streams. Only 4 of 16 fish species collected were native to the drainage. Fish assemblages of urbanized sites included two native species, arroyo chub Gila orcuttii and Santa Ana sucker Catostomus santaanae, at sites that were intermediate in coefficient of variation of bank-full width, depth, bed substrate, and water temperature. Alien species dominated urbanized sites with lesser or greater values for these variables. These results suggest that urban streams can be structured to enhance populations of native fishes. Continued study of urban streams in the Santa Ana River basin and elsewhere will contribute to the basic understanding of ecological principles and help preserve the maximum ecological value of streams in highly urbanized areas.

  10. High-resolution digital elevation model of Mount St. Helens crater and upper North Fork Toutle River basin, Washington, based on an airborne lidar survey of September 2009

    Science.gov (United States)

    Mosbrucker, Adam

    2014-01-01

    The lateral blast, debris avalanche, and lahars of the May 18th, 1980, eruption of Mount St. Helens, Washington, dramatically altered the surrounding landscape. Lava domes were extruded during the subsequent eruptive periods of 1980–1986 and 2004–2008. More than three decades after the emplacement of the 1980 debris avalanche, high sediment production persists in the North Fork Toutle River basin, which drains the northern flank of the volcano. Because this sediment increases the risk of flooding to downstream communities on the Toutle and Cowlitz Rivers, the U.S. Army Corps of Engineers (USACE), under the direction of Congress to maintain an authorized level of flood protection, built a sediment retention structure on the North Fork Toutle River in 1989 to help reduce this risk and to prevent sediment from clogging the shipping channel of the Columbia River. From September 16–20, 2009, Watershed Sciences, Inc., under contract to USACE, collected high-precision airborne lidar (light detection and ranging) data that cover 214 square kilometers (83 square miles) of Mount St. Helens and the upper North Fork Toutle River basin from the sediment retention structure to the volcano's crater. These data provide a digital dataset of the ground surface, including beneath forest cover. Such remotely sensed data can be used to develop sediment budgets and models of sediment erosion, transport, and deposition. The U.S. Geological Survey (USGS) used these lidar data to develop digital elevation models (DEMs) of the study area. DEMs are fundamental to monitoring natural hazards and studying volcanic landforms, fluvial and glacial geomorphology, and surface geology. Watershed Sciences, Inc., provided files in the LASer (LAS) format containing laser returns that had been filtered, classified, and georeferenced. The USGS produced a hydro-flattened DEM from ground-classified points at Castle, Coldwater, and Spirit Lakes. Final results averaged about five laser last

  11. 7-Years of Using Distributed Temperature Sensing (DTS) to assess river restoration efforts : synergies of high-resolution observation and modeling on the Middle Fork of the John Day River

    Science.gov (United States)

    Hall, A.; Diabat, M.

    2014-12-01

    Temperature is a key factor for salmonid health and is an important restoration metric on the Middle Fork of the John Day River, northeast Oregon. The longest undammed tributary to the Columbia, the headwaters of the Middle Fork are crucial to steelhead and spring Chinook and summer Chinook juvenile rearing. In the past century the river has been altered by dredge mining, overgrazing, logging activities, and irrigation resulting in bank erosion, low effective shade, and channelization. These factors decreased fish habitat and led to increased stream temperature maxima. Restoration has focused on restoring fish habitat, creating thermal refugia, and planting native vegetation. The most recent completed restoration project diverted the flow into the historic, meandering stream channel from the dredged, straightened channel. Over the past seven years, Oregon State University researchers (Tara O'Donnell-2012, Julie Huff-2009) have been involved in a planned-to-be 10-year stream temperature monitoring study to assess maximum temperatures during low-flow summer months. The use of fiber optics through distributed temperature sensing (DTS) made it possible to record high resolution temperature data at both temporal and spatial scales; data which is used to assess the efficacy of restoration efforts on the reach. Furthermore, DTS provided temperature data that reveals subtle hydrologic processes such as groundwater or hyporheic inflows and quantifies their effect on the stream. Current research has focused on large scale DTS installations on the Middle Fork of the John Day River on the Oxbow, Forrest, and the upstream Galena ("RPB") conservation properties. In the summers of 2013 and 2014, 16 km of river were monitored. Our study compares temperatures before and after the restoration project and provides essential guidance for future restoration projects. Direct comparisons coupled with a deterministic modeling using HeatSource assist in better understanding the

  12. Altered orbitofrontal sulcogyral patterns in adult males with high-functioning autism spectrum disorders.

    Science.gov (United States)

    Watanabe, Hiromi; Nakamura, Motoaki; Ohno, Taisei; Itahashi, Takashi; Tanaka, Eizaburo; Ohta, Haruhisa; Yamada, Takashi; Kanai, Chieko; Iwanami, Akira; Kato, Nobumasa; Hashimoto, Ryuichiro

    2014-04-01

    Functions of the orbitofrontal cortex include diverse social, cognitive and affective processes, many of which are abnormal in autism spectrum disorders (ASDs). Recently, altered orbitofrontal sulcogyral patterns have been revealed in several psychiatric conditions, such as schizophrenia, indicating a possibility that altered orbitofrontal sulcogyral morphology reflects abnormal neurodevelopment. However, the presence of sulcal alterations in ASD remains unexplored. Using structural magnetic resonance imaging, subtypes of the 'H-shaped' sulcus (Type I, II and III, in order of frequency), posterior orbital sulcus (POS) and intermediate orbital sulcus were identified in each hemisphere of adult males with ASD (n = 51) and matched normal controls (n = 55) based on the study by Chiavaras and Petrides. ASD showed a significantly altered distribution of H-shaped sulcal subtypes in both hemispheres, with a significant increase of Type III. A significant alteration in the distribution of sulcal subtypes was also identified in the right hemisphere POS of ASD. Categorical regression analysis revealed that Type I and II expressions predicted a reduced total Autism-Spectrum Quotient score. Furthermore, Type I expression was associated with a reduced 'attention to detail' subscale score. The results demonstrate that altered sulcogyral morphology can be a marker for abnormal neurodevelopment leading to the increased risk of developing autism.

  13. Design and maintenance of a network for collecting high-resolution suspended-sediment data at remote locations on rivers, with examples from the Colorado River

    Science.gov (United States)

    Griffiths, Ronald E.; Topping, David J.; Andrews, Timothy; Bennett, Glenn E.; Sabol, Thomas A.; Melis, Theodore S.

    2012-01-01

    Management of sand and finer sediment in fluvial settings has become increasingly important for reasons ranging from endangered-species habitat to transport of sediment-associated contaminants. In all rivers, some fraction of the suspended load is transported as washload, and some as suspended bed material. Typically, the washload is composed of silt-and-clay-size sediment, and the suspended bed material is composed of sand-size sediment. In most rivers, as a result of changes in the upstream supply of silt and clay, large, systematic changes in the concentration of the washload occur over time, independent of changes in water discharge. Recent work has shown that large, systematic, discharge-independent changes in the concentration of the suspended bed material are also present in many rivers. In bedrock canyon rivers, such as the Colorado River in Grand Canyon National Park, changes in the upstream tributary supply of sand may cause large changes in the grain-size distribution of the bed sand, resulting in changes in both the concentration and grain-size distribution of the sand in suspension. Large discharge-independent changes in suspended-sand concentration coupled to discharge-independent changes in the grain-size distribution of the suspended sand are not unique to bedrock canyon rivers, but also occur in large alluvial rivers, such as the Mississippi River. These systematic changes in either suspended-silt-and-clay concentration or suspended-sand concentration may not be detectable by using conventional equal-discharge- or equal-width-increment measurements, which may be too infrequently collected relative to the time scale over which these changes in the sediment load are occurring. Furthermore, because large discharge-independent changes in both suspended-silt-and-clay and suspended-sand concentration are possible in many rivers, methods using water discharge as a proxy for suspended-sediment concentration (such as sediment rating curves) may not produce

  14. The risk of river pollution due to washout from contaminated floodplain water bodies during high floods

    Science.gov (United States)

    Lyubimova, Tatyana; Lepikhin, Anatoly; Parshakova, Yanina; Tiunov, Alexey

    2016-04-01

    Today, the potential impact of extremely high floods, which in the last years have become a rather frequent weather-related disaster, is the problem of primary concern. In studies of the potential impact of floods the emphasis is placed first of all on the estimation of possible flood zones and the analysis of the flow regimes in these zones. However, in some cases the hydrochemical parameters related to changes in the chemical composition of water are more important than the hydraulic parameters. It is generally believed that the higher is the flow rate, the more intensive is the process of dissolution, i.e. the lower is the concentration of limiting contaminants in water. However, this statement is valid provided that flooding does not activate new sources of water pollution such as contaminated floodplain water bodies located in the vicinity of water supply systems. Being quite reliable and safe at small and moderate discharges, in the case of extremely high level of river waters they become intensive sources of water pollution, essentially limiting the water consumption schedule for downstream water consumers. It should be noted that compared to the well-studied mechanisms of waste discharge due to failure of hydraulic engineering structures by flood waves, the mechanisms of pollutant washout from the contaminated floodplain water bodies by the flood waves is still poorly understood. We analyze the impacts of such weather-related events on the quality of water in the water intake system, taking as an example, the section of the Vyatka River located in the Prikamskaya lowland of the Russian Federation. The risk of river pollution due to washout from the contaminated floodplain water bodies during high floods is studied by hydrodynamical modeling in the framework of combined approach using one-, two- and three-dimensional hydrodynamic models are implemented and by in situ measurements. It is shown that during high floods the removal of pollutants from the

  15. Using High Spatio-Temporal Optical Remote Sensing to Monitor Dissolved Organic Carbon in the Arctic River Yenisei

    Directory of Open Access Journals (Sweden)

    Pierre-Alexis Herrault

    2016-09-01

    Full Text Available In Arctic regions, a major concern is the release of carbon from melting permafrost that could greatly exceed current human carbon emissions. Arctic rivers drain these organic-rich watersheds (Ob, Lena, Yenisei, Mackenzie, Yukon but field measurements at the outlets of these great Arctic rivers are constrained by limited accessibility of sampling sites. In particular, the highest dissolved organic carbon (DOC fluxes are observed throughout the ice breakup period that occurs over a short two to three-week period in late May or early June during the snowmelt-generated peak flow. The colored fraction of dissolved organic carbon (DOC which absorbs UV and visible light is designed as chromophoric dissolved organic matter (CDOM. It is highly correlated to DOC in large arctic rivers and streams, allowing for remote sensing to monitor DOC concentrations from satellite imagery. High temporal and spatial resolutions remote sensing tools are highly relevant for the study of DOC fluxes in a large Arctic river. The high temporal resolution allows for correctly assessing this highly dynamic process, especially the spring freshet event (a few weeks in May. The high spatial resolution allows for assessing the spatial variability within the stream and quantifying DOC transfer during the ice break period when the access to the river is almost impossible. In this study, we develop a CDOM retrieval algorithm at a high spatial and a high temporal resolution in the Yenisei River. We used extensive DOC and DOM spectral absorbance datasets from 2014 and 2015. Twelve SPOT5 (Take5 and Landsat 8 (OLI images from 2014 and 2015 were examined for this investigation. Relationships between CDOM and spectral variables were explored using linear models (LM. Results demonstrated the capacity of a CDOM algorithm retrieval to monitor DOC fluxes in the Yenisei River during a whole open water season with a special focus on the peak flow period. Overall, future Sentinel2/Landsat8

  16. High fat diet alters lactation outcomes: possible involvement of inflammatory and serotonergic pathways.

    Directory of Open Access Journals (Sweden)

    Laura L Hernandez

    Full Text Available Delay in the onset of lactogenesis has been shown to occur in women who are obese, however the mechanism altered within the mammary gland causing the delay remains unknown. Consumption of high fat diets (HFD has been previously determined to result decreased litters and litter numbers in rodent models due to a decrease in fertility. We examined the effects of feeding a HFD (60% kcal from fat diet versus a low-fat diet (LFD; 10% kcal from fat to female Wistar rats on lactation outcomes. Feeding of HFD diet resulted in increased pup weights compared to pups from LFD fed animals for 4 d post-partum. Lactation was delayed in mothers on HFD but they began to produce copious milk volumes beginning 2 d post-partum, and milk yield was similar to LFD by day 3. Mammary glands collected from lactating animals on HFD diet, displayed a disrupted morphologies, with very few and small alveoli. Consistently, there was a significant decrease in the mRNA expression of milk protein genes, glucose transporter 1 (GLUT1 and keratin 5 (K5, a luminobasal cell marker in the mammary glands of HFD lactating animals. Expression of tryptophan hydroxylase 1 (TPH1, the rate-limiting enzyme in serotonin (5-HT biosynthesis, and the 5-HT(7 receptor (HTR7, which regulates mammary gland involution, were significantly increased in mammary glands of HFD animals. Additionally, we saw elevation of the inflammatory markers interleukin-6 (IL-6 and tumor necrosis factor-α (TNF- α. These results indicate that consumption of HFD impairs mammary parenchymal tissue and impedes its ability to synthesize and secrete milk, possibly through an increase in 5-HT production within the mammary gland leading to an inflammatory process.

  17. High-throughput sperm differential proteomics suggests that epigenetic alterations contribute to failed assisted reproduction.

    Science.gov (United States)

    Azpiazu, Rubén; Amaral, Alexandra; Castillo, Judit; Estanyol, Josep Maria; Guimerà, Marta; Ballescà, Josep Lluís; Balasch, Juan; Oliva, Rafael

    2014-06-01

    Are there quantitative alterations in the proteome of normozoospermic sperm samples that are able to complete IVF but whose female partner does not achieve pregnancy? Normozoospermic sperm samples with different IVF outcomes (pregnancy versus no pregnancy) differed in the levels of at least 66 proteins. The analysis of the proteome of sperm samples with distinct fertilization capacity using low-throughput proteomic techniques resulted in the detection of a few differential proteins. Current high-throughput mass spectrometry approaches allow the identification and quantification of a substantially higher number of proteins. This was a case-control study including 31 men with normozoospermic sperm and their partners who underwent IVF with successful fertilization recruited between 2007 and 2008. Normozoospermic sperm samples from 15 men whose female partners did not achieve pregnancy after IVF (no pregnancy) and 16 men from couples that did achieve pregnancy after IVF (pregnancy) were included in this study. To perform the differential proteomic experiments, 10 no pregnancy samples and 10 pregnancy samples were separately pooled and subsequently used for tandem mass tags (TMT) protein labelling, sodium dodecyl sulphate-polyacrylamide gel electrophoresis, liquid chromatography tandem mass spectrometry (LC-MS/MS) identification and peak intensity relative protein quantification. Bioinformatic analyses were performed using UniProt Knowledgebase, DAVID and Reactome. Individual samples (n = 5 no pregnancy samples; n = 6 pregnancy samples) and aliquots from the above TMT pools were used for western blotting. By using TMT labelling and LC-MS/MS, we have detected 31 proteins present at lower abundance (ratio no pregnancy/pregnancy 1.5) in the no pregnancy group. Bioinformatic analyses showed that the proteins with differing abundance are involved in chromatin assembly and lipoprotein metabolism (P values Economia y Competividad; FEDER BFU 2009-07118 and PI13/00699) and

  18. Androgen Deficiency Exacerbates High-Fat Diet-Induced Metabolic Alterations in Male Mice.

    Science.gov (United States)

    Dubois, Vanessa; Laurent, Michaël R; Jardi, Ferran; Antonio, Leen; Lemaire, Katleen; Goyvaerts, Lotte; Deldicque, Louise; Carmeliet, Geert; Decallonne, Brigitte; Vanderschueren, Dirk; Claessens, Frank

    2016-02-01

    Androgen deficiency is associated with obesity, metabolic syndrome, and type 2 diabetes mellitus in men, but the mechanisms behind these associations remain unclear. In this study, we investigated the combined effects of androgen deficiency and high-fat diet (HFD) on body composition and glucose homeostasis in C57BL/6J male mice. Two models of androgen deficiency were used: orchidectomy (ORX) and androgen receptor knockout mice. Both models displayed higher adiposity and serum leptin levels upon HFD, whereas no differences were seen on a regular diet. Fat accumulation in HFD ORX animals was accompanied by increased sedentary behavior and occurred in spite of reduced food intake. HFD ORX mice showed white adipocyte hypertrophy, correlated with decreased mitochondrial content but not function as well as increased lipogenesis and decreased lipolysis suggested by the up-regulation of fatty acid synthase and the down-regulation of hormone-sensitive lipase. Both ORX and androgen receptor knockout exacerbated HFD-induced glucose intolerance by impairing insulin action in liver and skeletal muscle, as evidenced by the increased triglyceride and decreased glycogen content in these tissues. In addition, serum IL-1β levels were elevated, and pancreatic insulin secretion was impaired after ORX. Testosterone but not dihydrotestosterone supplementation restored the castration effects on body composition and glucose homeostasis. We conclude that sex steroid deficiency in combination with HFD exacerbates adiposity, insulin resistance, and β-cell failure in 2 preclinical male mouse models. Our findings stress the importance of a healthy diet in a clinical context of androgen deficiency and may have implications for the prevention of metabolic alterations in hypogonadal men.

  19. Early life stress-induced alterations in rat brain structures measured with high resolution MRI.

    Science.gov (United States)

    Sarabdjitsingh, R Angela; Loi, Manila; Joëls, Marian; Dijkhuizen, Rick M; van der Toorn, Annette

    2017-01-01

    Adverse experiences early in life impair cognitive function both in rodents and humans. In humans this increases the vulnerability to develop mental illnesses while in the rodent brain early life stress (ELS) abnormalities are associated with changes in synaptic plasticity, excitability and microstructure. Detailed information on the effects of ELS on rodent brain structural integrity at large and connectivity within the brain is currently lacking; this information is highly relevant for understanding the mechanism by which early life stress predisposes to mental illnesses. Here, we exposed rats to 24 hours of maternal deprivation (MD) at postnatal day 3, a paradigm known to increase corticosterone levels and thereby activate glucocorticoid receptors in the brain. Using structural magnetic resonance imaging we examined: i) volumetric changes and white/grey matter properties of the whole cerebrum and of specific brain areas; and ii) whether potential alterations could be normalized by blocking glucocorticoid receptors with mifepristone during the critical developmental window of early adolescence, i.e. between postnatal days 26 and 28. The results show that MD caused a volumetric reduction of the prefrontal cortex, particularly the ventromedial part, and the orbitofrontal cortex. Within the whole cerebrum, white (relative to grey) matter volume was decreased and region-specifically in prefrontal cortex and dorsomedial striatum following MD. A trend was found for the hippocampus. Grey matter fractions were not affected. Treatment with mifepristone did not normalize these changes. This study indicates that early life stress in rodents has long lasting consequences for the volume and structural integrity of the brain. However, changes were relatively modest and-unlike behavior- not mitigated by blockade of glucocorticoid receptors during a critical developmental period.

  20. High resolution modelling of the biogeochemical processes in the eutrophic Loire River (France)

    Science.gov (United States)

    Minaudo, Camille; Moatar, Florentina; Curie, Florence; Gassama, Nathalie; Billen, Gilles

    2016-04-01

    A biogeochemical model was developed, coupling a physically based water temperature model (T-NET) with a semi-mechanistic biogeochemical model (RIVE, used in ProSe and Riverstrahler models) in order to assess at a fine temporal and spatial resolution the biogeochemical processes in the eutrophic Middle Loire hydrosystem (≈10 000 km², 3361 river segments). The code itself allows parallelized computing, which decreased greatly the calculation time (5 hours for simulating 3 years hourly). We conducted a daily survey during the period 2012-2014 at 2 sampling stations located in the Middle Loire of nutrients, chlorophyll pigments, phytoplankton and physic-chemical variables. This database was used as both input data (upstream Loire boundary) and validation data of the model (basin outlet). Diffuse and non-point sources were assessed based on a land cover analysis and WWTP datasets. The results appeared very sensible to the coefficients governing the dynamic of suspended solids and of phosphorus (sorption/desorption processes) within the model and some parameters needed to be estimated numerically. Both the Lagrangian point of view and fluxes budgets at the seasonal and event-based scale evidenced the biogeochemical functioning of the Loire River. Low discharge levels set up favorable physical conditions for phytoplankton growth (long water travel time, limited water depth, suspended particles sedimentation). Conversely, higher discharge levels highly limited the phytoplankton biomass (dilution of the colony, washing-out, limited travel time, remobilization of suspended sediments increasing turbidity), and most biogeochemical species were basically transferred downstream. When hydrological conditions remained favorable for phytoplankton development, P-availability was the critical factor. However, the model evidenced that most of the P in summer was recycled within the water body: on one hand it was assimilated by the algae biomass, and on the other hand it was

  1. Land-sea duel in the late Quaternary at the mouth of a small river with high sediment yield

    Science.gov (United States)

    Yang, Rick J.; Liu, James T.; Fan, Daidu; Burr, George S.; Lin, Hui-Ling; Chen, Ting-Ting

    2017-08-01

    The transition between sedimentary environments is compressed along land-sea boundaries in space and time. At river mouths on high standing islands, sedimentary records with high temporal resolutions formed as a result of large sediment loads and pervasive post-glacial sea level rise. Here we report on sediment core records covering the late Quaternary from the mouth of a small mountainous river in Taiwan. Results show that the study site was initially terrestrial under fluvial control. Beginning at about 10,000 yr BP (before present) the site became inundated by the rising sea and the environmental facies transitioned from a floodplain/incised river valley to a succession of marine environments, from shoreface to offshore. As the rising sea level came to a pause at 6000 yr BP, fluvial processes became dominant and sediments began to aggrade at the river mouth. After 4500 yr BP, the accumulated sediment began to prograde seaward, taking on the form of a river delta, and subtidal sand ridges appeared in the nearshore. This also introduces the deltaic development, which was limited by topography of the receiving basin. The chronology expresses the duel between sea level and fluvial processes that determined the depositional environments along the land-sea boundary at the study site.

  2. Functional and proteomic alterations of plasma high density lipoproteins in type 1 diabetes mellitus.

    Science.gov (United States)

    Manjunatha, Shankarappa; Distelmaier, Klaus; Dasari, Surendra; Carter, Rickey E; Kudva, Yogish C; Nair, K Sreekumaran

    2016-09-01

    Higher HDL-cholesterol (HDL-C) is linked to lower cardiovascular risk but individuals with type 1 diabetes mellitus (T1DM) with normal or high HDL-C have higher cardiovascular events compared to age matched non-diabetic controls (ND). We determined whether altered HDL functions despite having normal HDL-C concentration may explain increased cardiovascular risk in T1DM individuals. We also determined whether irreversible posttranslational modifications (PTMs) of HDL bound proteins occur in T1DM individuals with altered HDL functions. T1DM with poor glycemic control (T1D-PC, HbA1c≥8.5%, n=15) and T1DM with good glycemic control (T1D-GC, HbA1c≤6.6%, n=15) were compared with equal numbers of NDs, ND-PC and ND-GC respectively, matched for age, sex and body mass index (BMI). We measured cholesterol efflux capacity (CEC) of HDL in the serum using J774 macrophages, antioxidant function of HDL as the ability to reverse the oxidative damage of LDL and PON1 activity using commercially available kit. For proteomic analysis, HDL was isolated by density gradient ultracentrifugation and was analyzed by mass spectrometry and shotgun proteomics method. Plasma HDL-C concentrations in both T1DM groups were similar to their ND. However, CEC (%) of T1D-PC (16.9±0.8) and T1D-GC (17.1±1) were lower than their respective ND (17.9±1, p=0.01 and 18.2±1.4, p=0.02). HDL antioxidative function also was lower (p<0.05). The abundance of oxidative PTMs of apolipoproteins involved in CEC and antioxidative functions of HDL were higher in T1D-PC (ApoA4, p=0.041) and T1D-GC (ApoA4, p=0.025 and ApoE, p=0.041) in comparison with ND. Both T1D-PC and T1D-GC groups had higher abundance of amadori modification of ApoD (p=0.002 and p=0.041 respectively) and deamidation modification of ApoA4 was higher in T1D-PC (p=0.025). Compromised functions of HDL particles in T1DM individuals, irrespective of glycemic control, could be explained by higher abundance of irreversible PTMs of HDL proteins. These

  3. High-order chromatin architecture shapes the landscape of chromosomal alterations in cancer

    Science.gov (United States)

    Fudenberg, Geoffrey; Getz, Gad; Meyerson, Matthew; Mirny, Leonid

    2012-02-01

    The rapid growth of cancer genome structural information provides an opportunity for a better understanding of the mutational mechanisms of genomic alterations in cancer and the forces of selection that act upon them. Here we test the evidence for two major forces, spatial chromosome structure and purifying (or negative) selection, that shape the landscape of somatic copy-number alterations (SCNAs) in cancer (Beroukhim et al, 2010). Using a maximum likelihood framework we compare SCNA maps and three-dimensional genome architecture as determined by genome-wide chromosome conformation capture (HiC) and described by the proposed fractal-globule (FG) model (Lieberman-Aiden and Van Berkum et al, 2009). This analysis provides evidence that the distribution of chromosomal alterations in cancer is spatially related to three-dimensional genomic architecture and additionally suggests that purifying selection as well as positive selection shapes the landscape of SCNAs during somatic evolution of cancer cells.

  4. High-resololution topography for the analysis of palaeochannels in the Manawatu river (New Zealand)

    Science.gov (United States)

    Lo Re, Giulia; Fuller, Ian C.; Sofia, Giulia; Holt, Katerine; Macklin, Mark G.; Tarolli, Paolo

    2016-04-01

    In anthropogenic landscapes, the identification of palaeochannels provides an effective opportunity to improve current estimates of flood risk, extending with sediment analysis short instrumental records, and allowing for the definition of possible preferential flood flow dynamics. New remote sensing techniques in the last decades have led to a dramatic increase in terrain information (Tarolli, 2014), offering a new point of view for such type of landform analysis. Published literature includes a lot of applications of lidar data, and this trend is becoming more prominent due to the wider availability and the lower cost of lidar DTMs for the scientific community and environmental agencies. One of the great advances in using high-resolution topography (HRT) derived by lidar is the capability to provide an accurate and detailed recognition of terrain morphologies also under vegetation cover. The aim of this study is to use lidar HRT to automatically identify palaeochannels in the Manawatu river (New Zealand), flowing in a high-urbanized alluvial plain and for which a high-quality laser scanner data is available in addition to soil sample cores subject to sedimentology analysis and 14C-dating. The palaeochannels geomorphic feature recognition techniques considered in this study is based on a statistical analysis of morphometric parameters (Sofia et al. 2014). Further, the different topographic indices have been read along cross-sectional profiles for each extracted features, and this information together with the relative distance to the main river, combined with sediments analysis, were considered for a preliminary analysis of the relative age of the palaeochannels. The collected results highlighted the capability of high-resolution topography in the identification, mapping, and potentially dating of the palaeochannels in urbanized alluvial plains. In a large-scale context, it allowed to narrow down the area of investigation, thus reducing the cost and time involved

  5. Altered Carbon Isotope Discrimination of C3 Plants Under Very High pCO2 Levels

    Science.gov (United States)

    Panetta, R. J.; Schubert, B.; Jahren, H.

    2009-12-01

    Various modeling and proxy-based reconstructions of atmospheric pCO2 levels for the last 120 Ma have estimated RCO2 as high as 12x for the Early Cretaceous, generally decreasing into the Cenozoic, and decreasing further into the Quaternary. Multiple ecological studies to assess the effect of elevated CO2 on plant biomass and δ13C value have been spurred on by recent increases in greenhouse gases, however these studies typically grow plants under only slightly elevated CO2 levels (i.e., the twenty foremost studies published since 1990 involved 550 to 750 ppm pCO2, which equals RCO2 = 1.4 to 1.9x). In order to recreate the highest pCO2 environments of the last 120 Ma, we grew radish (Raphanus sativus L.) in growth chambers that maintained controlled environmental conditions and pCO2 levels ranging from ~5 to 11x that of today’s atmosphere (1791 to 4200 ppm); upon harvest we measured total biomass and stable carbon isotope ratio (δ13Cplant) in both above and below ground plant tissue. Unlike the 1:1 relationship between stable isotopes of atmospheric CO2 (δ13Catm) and δ13Cplant observed at lower pCO2 levels (i.e., RCO2 = 1x to 3x; Jahren et al., 2008), the δ13Cplant of biomass grown at more elevated RCO2 was dependent upon δ13Catm according to the linear relationship: δ13Cplant = 1.9(δ13Cplant) - 12.2 ‰ (r2 = 0.71). Concomitantly, we see a highly significant (p sativus L. from -27.0 to -28.0 ‰ at RCO2 = 5x to 11x, respectively. We will discuss possible mechanisms for changing isotope discrimination at very high pCO2 levels that may not be operative at lower concentrations. For example, we noted a striking reduction in the variability of biomass between plants grown at the same (very high) level of pCO2. This variability (calculated as the standard deviation of the log-transformed biomass data after Poorter and Garnier, 1996) decreased by 37 % (above-ground) and 48 % (below-ground) for plants grown at RCO2 > 5x compared to plants grown at RCO2 = 1x to 3x

  6. Water quality assessment of a highly polluted Mediterranean River - Oued Fez (Morocco)

    Science.gov (United States)

    Perrin, J.-L.; Bellarbi, M.; Raïs, N.; Chahinian, N.; Moulin, P.; Ijjaali, M.

    2012-04-01

    In the South of the Mediterranean basin, many rivers are characterized by an alternation of very long dry periods only cut by short flood events. Currently, the socio-economical development of these zones is limited by water scarcity and poor quality of the water resources. Indeed human activities, generally concentrated in overpopulated cities, generate large quantity of domestic and industrial effluents which are directly rejected in the environment without any treatment. In Morocco, the well known city of Fez illustrates perfectly this situation, observed in most developing countries. The oued Fez receives continuously the non-treated domestic and industrial effluents (90.000 m3/day) of the city and pollutes all the downstream water bodies. Indeed, it is a tributary of the Sebou River, a major body of great economical importance used for irrigation and freshwater supply. This study aims at characterising and quantifying the pollutant concentrations and fluxes in various points of oued Fez's hydrological network and assessing its impact on the Sebou River; this river's preservation being considered a national priority in Morocco. A coupled water quality-water quantity monitoring scheme has been implemented on oued Fez since 2008. In addition to basic hydrological data, water quality samples are collected at regular intervals at 8 locations where discharge is simultaneously measured using an Acoustic Doppler Current Profiler (ADCP). Water samples are analysed for different forms of nitrogen (nitrates, nitrites, ammonium and total nitrogen), phosphorus (soluble reactive phosphorus and total phosphorus) but also total chromium which is used in the leather tanning processes, one of the most important industrial production of the city of Fez, using a photospectrometer (Hach Lange DR 2800 VIS-photometer (Germany). The results of 17 sampling campaigns, carried out over 3 hydrological years, indicate that the rural areas contribute mostly to baseflow during the wet

  7. High-resolution computed tomography of the middle ear and mastoid. Part III. Surgically altered anatomy and pathology

    Energy Technology Data Exchange (ETDEWEB)

    Swartz, J.D.; Goodman, R.S.; Russell, K.B.; Ladenheim, S.F.; Wolfson, R.J.

    1983-08-01

    High-resolution computed tomography (CT) provides an excellent method for examination of the surgically altered middle ear and mastoid. Closed-cavity and open-cavity types of mastoidectomy are illustrated. Recurrent cholesteatoma in the mastoid bowl is easily diagnosed. Different types of tympanoplasty are discussed and illustrated, as are tympanostomy tubes and various ossicular reconstructive procedures. Baseline high-resolution CT of the postoperative middle ear and mastoid is recommended at approximately 3 months following the surgical procedure.

  8. Human longevity is characterised by high thyroid stimulating hormone secretion without altered energy metabolism

    DEFF Research Database (Denmark)

    Jansen, S W; Akintola, A A; Roelfsema, F;

    2015-01-01

    hormone (TH) in an inverse relationship. Greater longevity has been associated with higher TSH and lower TH levels, but mechanisms underlying TSH/TH differences and longevity remain unknown. The HPT axis plays a pivotal role in growth, development and energy metabolism. We report that offspring...... may favour longevity without altering energy metabolism....

  9. Can high resolution 3D topographic surveys provide reliable grain size estimates in gravel bed rivers?

    Science.gov (United States)

    Pearson, E.; Smith, M. W.; Klaar, M. J.; Brown, L. E.

    2017-09-01

    High resolution topographic surveys such as those provided by Structure-from-Motion (SfM) contain a wealth of information that is not always exploited in the generation of Digital Elevation Models (DEMs). In particular, several authors have related sub-metre scale topographic variability (or 'surface roughness') to sediment grain size by deriving empirical relationships between the two. In fluvial applications, such relationships permit rapid analysis of the spatial distribution of grain size over entire river reaches, providing improved data to drive three-dimensional hydraulic models, allowing rapid geomorphic monitoring of sub-reach river restoration projects, and enabling more robust characterisation of riverbed habitats. However, comparison of previously published roughness-grain-size relationships shows substantial variability between field sites. Using a combination of over 300 laboratory and field-based SfM surveys, we demonstrate the influence of inherent survey error, irregularity of natural gravels, particle shape, grain packing structure, sorting, and form roughness on roughness-grain-size relationships. Roughness analysis from SfM datasets can accurately predict the diameter of smooth hemispheres, though natural, irregular gravels result in a higher roughness value for a given diameter and different grain shapes yield different relationships. A suite of empirical relationships is presented as a decision tree which improves predictions of grain size. By accounting for differences in patch facies, large improvements in D50 prediction are possible. SfM is capable of providing accurate grain size estimates, although further refinement is needed for poorly sorted gravel patches, for which c-axis percentiles are better predicted than b-axis percentiles.

  10. High resolution aerosol optical thickness retrieval over the Pearl River Delta region with improved aerosol modelling

    Institute of Scientific and Technical Information of China (English)

    WONG; ManSing; NICHOL; Janet; LEE; Kwon; Ho

    2009-01-01

    Aerosol retrieval algorithms for the MODerate Resolution Imaging Spectroradiometer (MODIS) have been developed to estimate aerosol and microphysical properties of the atmosphere, which help to address aerosol climatic issues at global scale. However, higher spatial resolution aerosol products for urban areas have not been well researched mainly due to the difficulty of differentiating aerosols from bright surfaces in urban areas. Here, a new aerosol retrieval algorithm using the MODIS 500 m resolution images is described, to retrieve aerosol properties over Hong Kong and the Pearl River Delta region. The rationale of our technique is to first estimate the aerosol reflectance by decomposing the top-of-atmosphere reflectance from surface reflectance and Rayleigh path reflectance. For the determination of surface reflectance, a modified Minimum Reflectance Technique (MRT) is used, and MRT images are computed for different seasons. A strong correlation is shown between the surface reflectance of MRT images and MODIS land surface reflectance products (MOD09), with a value of 0.9. For conversion of aerosol reflectance to Aerosol Optical Thickness (AOT), comprehensive Look Up Tables (LUT) are constructed, in which aerosol properties and sun-viewing geometry in the radiative transfer calculations are taken into account. Four aerosol types, namely mixed urban, polluted urban, dust, and heavy pollution, were derived using cluster analysis on three years of AERONET measurements in Hong Kong. Their aerosol properties were input for LUT calculation. The resulting 500 m AOT images are highly correlated (r = 0.89) with AERONET sunphotometer observations in Hong Kong. This study demonstrates the applicability of aerosol retrieval at fine resolution scale in urban areas, which can assist the study of aerosol loading distribution and the impact of localized and transient pollution on urban air quality. In addition, the MODIS 500 m AOT images can be used to study cross

  11. High resolution aerosol optical thickness retrieval over the Pearl River Delta region with improved aerosol modelling

    Institute of Scientific and Technical Information of China (English)

    WONG ManSing; NICHOL Janet; LEE Kwon Ho; LI ZhanQing

    2009-01-01

    Aerosol retrieval algorithms for the MODerate Resolution Imaging Spectroradiometer (MODIS) have been developed to estimate aerosol and microphysical properties of the atmosphere, which help to address aerosol climatic issues at global scale. However, higher spatial resolution aerosol products for urban areas have not been well researched mainly due to the difficulty of differentiating aerosols from bright surfaces in urban areas. Here, a new aerosol retrieval algorithm using the MODIS 500 m resolu-tion images is described, to retrieve aerosol properties over Hong Kong and the Pearl River Delta re-gion. The rationale of our technique is to first estimate the aerosol reflectance by decomposing the top-of-atmosphere reflectance from surface reflectance and Rayleigh path reflectance. For the deter-mination of surface reflectance, a modified Minimum Reflectance Technique (MRT) is used, and MRT images are computed for different seasons. A strong correlation is shown between the surface reflec-tance of MRT images and MODIS land surface reflectance products (MOD09), with a value of 0.9. For conversion of aerosol reflectance to Aerosol Optical Thickness (AOT), comprehensive Look Up Tables (LUT) are constructed, in which aerosol properties and sun-viewing geometry in the radiative transfer calculations are taken into account. Four aerosol types, namely mixed urban, polluted urban, dust, and heavy pollution, were derived using cluster analysis on three years of AERONET measurements in Hong Kong. Their aerosol properties were input for LUT calculation. The resulting 500 m AOT images are highly correlated (r=0.89) with AERONET sunphotometer observations in Hong Kong. This study demonstrates the applicability of aerosol retrieval at fine resolution scale in urban areas, which can assist the study of aerosol loading distribution and the impact of localized and transient pollution on urban air quality. In addition, the MODIS 500 m AOT images can be used to study cross

  12. Signal Decomposition of High Resolution Time Series River Data to Separate Local and Regional Components of Conductivity

    Science.gov (United States)

    Signal processing techniques were applied to high-resolution time series data obtained from conductivity loggers placed upstream and downstream of an oil and gas wastewater treatment facility along a river. Data was collected over 14-60 days. The power spectral density was us...

  13. : Signal Decomposition of High Resolution Time Series River data to Separate Local and Regional Components of Conductivity

    Science.gov (United States)

    Signal processing techniques were applied to high-resolution time series data obtained from conductivity loggers placed upstream and downstream of a wastewater treatment facility along a river. Data was collected over 14-60 days, and several seasons. The power spectral densit...

  14. Contrasting climate change impact on river flows from high-altitude catchments in the Himalayan and Andes Mountains

    NARCIS (Netherlands)

    Ragettli, Silvan; Immerzeel, Walter W.; Pellicciotti, Francesca

    2016-01-01

    Mountain ranges are the world's natural water towers and provide water resources for millions of people. However, their hydrological balance and possible future changes in river flow remain poorly understood because of high meteorological variability, physical inaccessibility, and the complex interp

  15. The Availability and Utilization of School Library Resources in Some Selected Secondary Schools (High School) in Rivers State

    Science.gov (United States)

    Owate, C. N.; Iroha, Okpa

    2013-01-01

    This study investigates the availability and utilization of school library resources by Secondary School (High School) Students. Eight Selected Secondary Schools in Rivers State, Nigeria were chosen based on their performance in external examinations and geographic locations. In carrying out the research, questionnaires were administered to both…

  16. Using high-resolution phosphorus data to investigate mitigation measures in headwater river catchments

    Directory of Open Access Journals (Sweden)

    J. M. Campbell

    2014-09-01

    Full Text Available This study reports the use of high resolution water quality monitoring to assess the influence of changes in landuse management on total phosphorus (TP transfers in two 5 km2 agricultural sub-catchments. Specifically, the work investigates the "wicked problem" of agricultural soil P management and subsequent diffuse transfers at high river flows over a five year timescale. The work also investigates the phenomenon of low flow P pollution from septic tank systems (STS and mitigation efforts – here termed the "filthy issue" of rural catchment management. Results showed an inconsistent response to soil P management over five years with one catchment showing a convergence to optimum P concentrations and the other an overall increase. Both catchments indicated an overall increase in P concentration in defined high flow ranges. Low flow P concentration showed little change or higher P concentrations in defined low flow ranges despite replacement of defective systems and this is possibly due to a number of confounding reasons including increased housing densities due to new-builds. The work indicates fractured responses to catchment management advice and mitigation and that the short to medium term may be an insufficient time to expect the full implementation of policies (here defined as convergence to optimum soil P concentration and mitigation of STS and also to gauge their effectiveness.

  17. Using high-resolution phosphorus data to investigate mitigation measures in headwater river catchments

    Science.gov (United States)

    Campbell, J. M.; Jordan, P.; Arnscheidt, J.

    2014-09-01

    This study reports the use of high resolution water quality monitoring to assess the influence of changes in landuse management on total phosphorus (TP) transfers in two 5 km2 agricultural sub-catchments. Specifically, the work investigates the "wicked problem" of agricultural soil P management and subsequent diffuse transfers at high river flows over a five year timescale. The work also investigates the phenomenon of low flow P pollution from septic tank systems (STS) and mitigation efforts - here termed the "filthy issue" of rural catchment management. Results showed an inconsistent response to soil P management over five years with one catchment showing a convergence to optimum P concentrations and the other an overall increase. Both catchments indicated an overall increase in P concentration in defined high flow ranges. Low flow P concentration showed little change or higher P concentrations in defined low flow ranges despite replacement of defective systems and this is possibly due to a number of confounding reasons including increased housing densities due to new-builds. The work indicates fractured responses to catchment management advice and mitigation and that the short to medium term may be an insufficient time to expect the full implementation of policies (here defined as convergence to optimum soil P concentration and mitigation of STS) and also to gauge their effectiveness.

  18. Parameterization of High Resolution Vegetation Characteristics using Remote Sensing Products for the Nakdong River Watershed, Korea

    Directory of Open Access Journals (Sweden)

    Hyun Il Choi

    2013-01-01

    Full Text Available Mesoscale regional climate models (RCMs, the primary tool for climate predictions, have recently increased in sophistication and are being run at increasingly higher resolutions to be also used in climate impact studies on ecosystems, particularly in agricultural crops. As satellite remote sensing observations of the earth terrestrial surface become available for assimilation in RCMs, it is possible to incorporate complex land surface processes, such as dynamics of state variables for hydrologic, agricultural and ecologic systems at the smaller scales. This study focuses on parameterization of vegetation characteristics specifically designed for high resolution RCM applications using various remote sensing products, such as Advanced Very High Resolution Radiometer (AVHRR, Système Pour l’Observation de la Terre-VEGETATION (SPOT-VGT and Moderate Resolution Imaging Spectroradiometer (MODIS. The primary vegetative parameters, such as land surface characteristics (LCC, fractional vegetation cover (FVC, leaf area index (LAI and surface albedo localization factors (SALF, are currently presented over the Nakdong River Watershed domain, Korea, based on 1-km remote sensing satellite data by using the Geographic Information System (GIS software application tools. For future high resolution RCM modeling efforts on climate-crop interactions, this study has constructed the deriving parameters, such as FVC and SALF, following the existing methods and proposed the new interpolation methods to fill missing data with combining the regression equation and the time series trend function for time-variant parameters, such as LAI and NDVI data at 1-km scale.

  19. Landscape elements and river chemistry as affected by river regulation – a 3-D perspective

    Directory of Open Access Journals (Sweden)

    E. Smedberg

    2008-11-01

    Full Text Available We tested the hypothesis whether individual land classes within a river catchment contribute equally to river loading with dissolved constituents or whether some land classes act as "hot spots" to river loading and if so, are these land classes especially affected by hydrological alterations. The amount of land covered by forests and wetlands and the average soil depth of a river catchment explain 58–93% of the variability in total organic carbon (TOC and dissolved silicate (DSi concentrations for 22 river catchments in Northern Sweden. Whereas only 3% of the headwater areas of the Luleälven have been inundated by the creation of reservoirs, some 10% of the soils and aggregated forest and wetland areas have been lost due to damming and further hydrological alteration such as bypassing entire sub-catchments by headrace tunnels. However, looking at individual forest classes, our estimates indicate that some 37% of the deciduous forests have been inundated by the four major reservoirs built in the Luleälven headwaters. These deciduous forest and wetlands formerly growing on top of alluvial deposits along the river corridors forming the riparian zone play a vital role in loading river water with dissolved constituents, especially DSi. A digital elevation model draped with land classes and soil depths which highlights that topography of various land classes acting as hot spots is critical in determining water residence time in soils and biogeochemical fluxes. Thus, headwater areas of the Luleälven appear to be most sensitive to hydrological alterations due to the thin soil cover (on average 2.7–4.5 m and only patchy appearance of forest and wetlands that were significantly perturbed. Moreover, since these headwater areas are characterized often by high specific discharge, this relatively minor change in the landscape when compared to the entire river catchment may indeed explain the significant lower fluxes at the river mouth.

  20. Investigating riparian groundwater flow close to a losing river using diurnal temperature oscillations at high vertical resolution

    Directory of Open Access Journals (Sweden)

    T. Vogt

    2012-02-01

    Full Text Available River-water infiltration is of high relevance for hyporheic and riparian groundwater ecology as well as for drinking water supply by river-bank filtration. Heat has become a popular natural tracer to estimate exchange rates between rivers and groundwater. However, quantifying flow patterns and velocities is impeded by spatial and temporal variations of exchange fluxes, insufficient sensors spacing during field investigations, or simplifying assumptions for analysis or modeling such as uniform flow. The objective of this study is to investigate lateral shallow groundwater flow upon river-water infiltration at the shoreline of the riverbed and in the adjacent riparian zone of the River Thur in northeast Switzerland. Here we have applied distributed temperature sensing (DTS along optical fibers wrapped around tubes to measure high-resolution vertical temperature profiles of the unsaturated zone and shallow riparian groundwater. Diurnal temperature oscillations were tracked in the subsurface and analyzed by means of dynamic harmonic regression to extract amplitudes and phase angles. Subsequent calculations of amplitude attenuation and time shift relative to the river signal show in detail vertical and temporal variations of heat transport in shallow riparian groundwater. In addition, we apply a numerical two-dimensional heat transport model for the unsaturated zone and shallow groundwater to obtain a better understanding of the observed heat transport processes in shallow riparian groundwater and to estimate the groundwater flow velocity. Our results show that the observed riparian groundwater temperature distribution cannot be described by uniform flow, but rather by horizontal groundwater flow velocities varying over depth. In addition, heat transfer of diurnal temperature oscillations from the losing river through shallow groundwater is influenced by thermal exchange with the unsaturated zone. Neglecting the influence of the unsaturated zone

  1. Induction and reversion process of molecular and cytological alterations after highly irradiated food ingestion in mice

    Energy Technology Data Exchange (ETDEWEB)

    Rojo M, M.I. (Comision Chilena de Energia Nuclear, Santiago. Dept. de Aplicaciones de los Isotopos y Radiaciones); Fernandez C, M. (Chile Univ., Santiago. Dept. de Fisica)

    1984-04-01

    The molecular and cytological alterations induced in a mammal (Mus musculus) fed ad libitum with a balanced pellet diet irradiated with 50 KGy gamma radiation from weaning, for different periods, are analyzed. The transient chromosomal changes that recall tumor-like phenomena could be the expression of the damage and repair processes induced by changed molecules present in irradiated food. The reversible alterations of DNA structure and cytoplasmatic soluble proteins observed in mice fed with irradiated pellet diet could be interpreted as a result of the enhancement of the repair processes which could also explain the significant increase of the radioresistance of DNA found at 200 days after irradiated food ingestion. Finally, our results would suggest an induction of a pseudo-neoplasia due to a prolongated and exclusive ingestion of food irradiated with sterilizing gamma dose. Moreover, the maintenance of the irradiated diet induce the reversion of the observed phenomena by an eventual activation of the repair mechanisms.

  2. High precision terrestrial laser scanning : measuring the individual impact of floods and landslides on meandering bedrock river dynamics (Rangitikei river, New-Zealand)

    Science.gov (United States)

    Lague, D.; Bonnet, S.; Brodu, N.; Davies, T.; Leroux, J.

    2012-04-01

    Actively incising and meandering bedrock rivers are an ubiquitous feature of mountain belts, but the mechanisms leading to their formation and evolution are still poorly understood. Part of the problem lies in our limited ability to quantify in situ the mechanisms governing the dynamics of straight bedrock rivers (tool-cover effects, stochastic sediment supply and discharge effects, roughness effects,...). But the rapid and spatially variable rates of bank erosion in meanders also lead to potentially more complex hillslope-channel interactions than in straight river. Terrestrial laser scanner has the potential to shed new light on these interactions and to capture over large surfaces the impact of individual flood and landsliding events. Given the small changes of surface position that must be detected for even the largest rates of bedrock erosion (~ 5-10 mm/yr) and the 3D geometrical complexity of bedrock meanders, specific survey approaches and post-processing algorithms must be developed. To this end, we started to perform repeated Terrestrial Laser Scanner (TLS) surveys of actively incising meanders in the Rangitikei river (New-Zealand) in 2009. The Rangitikei river is incising weakly consolidated mudstone at an average rate of 5 mm/yr since 15 kyr and has developed a very sinuous meandering pattern with several cut-off bedrock meanders. Lateral undercutting of 100 m high cliffs generates failures of up to hundred of meters. Alluvial material consists of coarse resistant material (D50 ~20 cm) sourced from upstream, and large boulders locally derived from rockfalls. Exposed bedrock is rare on the bed but shows that abrasion, weathering by wetting-drying cycles and plucking are important incision mechanisms. Six TLS surveys were performed at bi-monthly to yearly intervals with a Leica Scanstation 2 in 5 reaches of variable planform curvature. Survey length varies from 300 m to 1200 m and point spacing from ~ 5 mm to 5 cm. Point clouds were co-registered between

  3. Pyrosequencing of antibiotic-contaminated river sediments reveals high levels of resistance and gene transfer elements.

    Directory of Open Access Journals (Sweden)

    Erik Kristiansson

    Full Text Available The high and sometimes inappropriate use of antibiotics has accelerated the development of antibiotic resistance, creating a major challenge for the sustainable treatment of infections world-wide. Bacterial communities often respond to antibiotic selection pressure by acquiring resistance genes, i.e. mobile genetic elements that can be shared horizontally between species. Environmental microbial communities maintain diverse collections of resistance genes, which can be mobilized into pathogenic bacteria. Recently, exceptional environmental releases of antibiotics have been documented, but the effects on the promotion of resistance genes and the potential for horizontal gene transfer have yet received limited attention. In this study, we have used culture-independent shotgun metagenomics to investigate microbial communities in river sediments exposed to waste water from the production of antibiotics in India. Our analysis identified very high levels of several classes of resistance genes as well as elements for horizontal gene transfer, including integrons, transposons and plasmids. In addition, two abundant previously uncharacterized resistance plasmids were identified. The results suggest that antibiotic contamination plays a role in the promotion of resistance genes and their mobilization from environmental microbes to other species and eventually to human pathogens. The entire life-cycle of antibiotic substances, both before, under and after usage, should therefore be considered to fully evaluate their role in the promotion of resistance.

  4. Antimicrobial activity of filamentous fungi isolated from highly antibiotic-contaminated river sediment

    Directory of Open Access Journals (Sweden)

    K. Stefan Svahn

    2012-05-01

    Full Text Available Background: Filamentous fungi are well known for their production of substances with antimicrobial activities, several of which have formed the basis for the development of new clinically important antimicrobial agents. Recently, environments polluted with extraordinarily high levels of antibiotics have been documented, leading to strong selection pressure on local sentinel bacterial communities. In such microbial ecosystems, where multidrug-resistant bacteria are likely to thrive, it is possible that certain fungal antibiotics have become less efficient, thus encouraging alternative strategies for fungi to compete with bacteria. Methods: In this study, sediment of a highly antibiotic-contaminated Indian river was sampled in order to investigate the presence of cultivable filamentous fungi and their ability to produce substances with antimicrobial activity. Results: Sixty one strains of filamentous fungi, predominantly various Aspergillus spp. were identified. The majority of the Aspergillus strains displayed antimicrobial activity against methicillin-resistant Staphylococcus aureus, extended-spectrum beta-lactamase-producing Escherichia coli, vancomycin-resistant Enterococcus faecalis and Candida albicans. Bioassay-guided isolation of the secondary metabolites of A. fumigatus led to the identification of gliotoxin. Conclusion: This study demonstrated proof of principle of using bioassay-guided isolation for finding bioactive molecules.

  5. The aquatic turtle assemblage inhabiting a highly altered landscape in southeast Missouri

    Science.gov (United States)

    Glorioso, Brad M.; Vaughn, Allison J.; Waddle, J. Hardin

    2010-01-01

    Turtles are linked to energetic food webs as both consumers of plants and animals and prey for many species. Turtle biomass in freshwater systems can be an order of magnitude greater than that of endotherms. Therefore, declines in freshwater turtle populations can change energy transfer in freshwater systems. Here we report on a mark–recapture study at a lake and adjacent borrow pit in a relict tract of bottomland hardwood forest in the Mississippi River floodplain in southeast Missouri, which was designed to gather baseline data, including sex ratio, size structure, and population size, density, and biomass, for the freshwater turtle population. Using a variety of capture methods, we captured seven species of freshwater turtles (snapping turtle Chelydra serpentina; red-eared slider Trachemys scripta; southern painted turtle Chrysemys dorsalis; river cooter Pseudemys concinna; false map turtle Graptemys pseudogeographica; eastern musk turtle Sternotherus odoratus; spiny softshell Apalone spinifera) comprising four families (Chelydridae, Emydidae, Kinosternidae, Trinoychidae). With the exception of red-eared sliders, nearly all individuals captured were adults. Most turtles were captured by baited hoop-nets, and this was the only capture method that caught all seven species. The unbaited fyke net was very successful in the borrow pit, but only captured four of the seven species. Basking traps and deep-water crawfish nets had minimal success. Red-eared sliders had the greatest population estimate (2,675), density (205/ha), and biomass (178 kg/ha). Two species exhibited a sex-ratio bias: snapping turtles C. serpentina in favor of males, and spiny softshells A. spinifera in favor of females.

  6. Contrasting climate change impact on river flows from high altitude catchments in the Himalayan and Andes Mountains

    OpenAIRE

    Ragettli, Silvan; Immerzeel, Walter; Pellicciotti, Francesca

    2016-01-01

    Mountain ranges are world’s natural water towers and provide water resources for millions of people. Yet, their hydrological balance and possible future changes in river flow remain poorly understood because of high meteorological variability, physical inaccessibility and the complex interplay between climate, cryosphere and hydrological processes. Here we use a state-of-the art glacio-hydrological model informed by data from high altitude observations and CMIP5 climate change scenarios to qu...

  7. Impact of the exceptionally high flood from the Changjiang River on the aquatic chemical distributions on the Huanghai Sea and East China Sea shelves in the summer of 1998

    Institute of Scientific and Technical Information of China (English)

    WANG Baodong; WANG Xiulin

    2006-01-01

    On the basis of the field observation in the Huanghai Sea and East China Sea in the summer of 1998, a rare event of exceptionally high discharge from the Changjiang River was described and how this high discharge altered water masses as well as chemical distributions on the shelves of the Huanghai Sea and East China Sea. The maximal extending ranges of the Changjiang diluted water and the nutrients in the freshwater from the Changjiang River were recorded for the first time. It was also found that there was a closed area with high oxygen and pH values in the offshore area of the southern Huanghai Sea and the northern East China Sea, indicating that the extensive spreading of nutrients due to the high discharge led to photosynthesis of phytoplankton mostly taking place in the offshore area far from the river mouth. The presence of "excess nitrogen" in almost all the northern East China Sea and the south of the Huanghai Sea suggests that these areas are potentially phosphorus-limited rather than nitrogen-limited, manifesting more like an estuarine ecosystem rather than a common marine ecosystem.

  8. Highly sensitivity adhesion molecules detection in hereditary haemochromatosis patients reveals altered expression.

    LENUS (Irish Health Repository)

    Norris, S

    2012-02-01

    Several abnormalities in the immune status of patients with hereditary haemochromatosis (HH) have been reported, suggesting an imbalance in their immune function. This may include persistent production of, or exposure to, altered immune signalling contributing to the pathogenesis of this disorder. Adhesion molecules L-, E- and P-Selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) are some of the major regulators of the immune processes and altered levels of these proteins have been found in pathological states including cardiovascular diseases, arthritis and liver cancer. The aim of this study was to assess L-, E- and P-Selectin, ICAM-1 and VCAM-1 expression in patients with HH and correlate these results with HFE mutation status and iron indexes. A total of 139 subjects were diagnosed with HH (C282Y homozygotes = 87, C282Y\\/H63D = 26 heterozygotes, H63D homozygotes = 26), 27 healthy control subjects with no HFE mutation (N\\/N), 18 normal subjects heterozygous for the H63D mutation served as age-sex-matched controls. We observed a significant decrease in L-selectin (P = 0.0002) and increased E-selectin and ICAM-1 (P = 0.0006 and P = 0.0059) expression in HH patients compared with healthy controls. This study observes for the first time that an altered adhesion molecules profile occurs in patients with HH that is associated with specific HFE genetic component for iron overload, suggesting that differential expression of adhesion molecules may play a role in the pathogenesis of HH.

  9. High-resolution genomic profiling of chronic lymphocytic leukemia reveals new recurrent genomic alterations.

    Science.gov (United States)

    Edelmann, Jennifer; Holzmann, Karlheinz; Miller, Florian; Winkler, Dirk; Bühler, Andreas; Zenz, Thorsten; Bullinger, Lars; Kühn, Michael W M; Gerhardinger, Andreas; Bloehdorn, Johannes; Radtke, Ina; Su, Xiaoping; Ma, Jing; Pounds, Stanley; Hallek, Michael; Lichter, Peter; Korbel, Jan; Busch, Raymonde; Mertens, Daniel; Downing, James R; Stilgenbauer, Stephan; Döhner, Hartmut

    2012-12-06

    To identify genomic alterations in chronic lymphocytic leukemia (CLL), we performed single-nucleotide polymorphism-array analysis using Affymetrix Version 6.0 on 353 samples from untreated patients entered in the CLL8 treatment trial. Based on paired-sample analysis (n = 144), a mean of 1.8 copy number alterations per patient were identified; approximately 60% of patients carried no copy number alterations other than those detected by fluorescence in situ hybridization analysis. Copy-neutral loss-of-heterozygosity was detected in 6% of CLL patients and was found most frequently on 13q, 17p, and 11q. Minimally deleted regions were refined on 13q14 (deleted in 61% of patients) to the DLEU1 and DLEU2 genes, on 11q22.3 (27% of patients) to ATM, on 2p16.1-2p15 (gained in 7% of patients) to a 1.9-Mb fragment containing 9 genes, and on 8q24.21 (5% of patients) to a segment 486 kb proximal to the MYC locus. 13q deletions exhibited proximal and distal breakpoint cluster regions. Among the most common novel lesions were deletions at 15q15.1 (4% of patients), with the smallest deletion (70.48 kb) found in the MGA locus. Sequence analysis of MGA in 59 samples revealed a truncating mutation in one CLL patient lacking a 15q deletion. MNT at 17p13.3, which in addition to MGA and MYC encodes for the network of MAX-interacting proteins, was also deleted recurrently.

  10. [Selective alteration of the declarative memory systems in patients treated with a high number of electroconvulsive therapy sessions].

    Science.gov (United States)

    Rami-González, L; Boget-Llucià, T; Bernardo, M; Marcos, T; Cañizares-Alejos, S; Penadés, R; Portella, M J; Castelví, M; Raspall, T; Salamero, M

    The reversible electrochemical effects of electroconvulsive therapy (ECT) on specific areas of the brain enable the neuroanatomical bases of some cognitive functions to be studied. In research carried out on memory systems, a selective alteration of the declarative ones has been observed after treatment with ECT. Little work has been done to explore the differential alteration of the memory subsystems in patients with a high number of ECT sessions. AIM. To study the declarative and non declarative memory system in psychiatric patients submitted to maintenance ECT treatment, with a high number of previous ECT sessions. 20 patients submitted to treatment with ECT (10 diagnosed as having depression and 10 with schizophrenia) and 20 controls, who were paired by age, sex and psychopathological diagnosis. For the evaluation of the declarative memory system, the Wechsler Memory Scale (WMS) logical memory test was used. The Hanoi Tower procedural test was employed to evaluate the non declarative system. Patients treated with ECT performed worse in the WMS logical memory test, but this was only significant in patients diagnosed as suffering from depression. No significant differences were observed in the Hanoi Tower test. A selective alteration of the declarative systems was observed in patients who had been treated with a high number of ECT sessions, while the non declarative memory systems remain unaffected.

  11. Wandering gravel-bed rivers and high-constructive stable channel sandy fluvial systems in the Ross River area, Yukon Territory, Canada

    Directory of Open Access Journals (Sweden)

    Darrel G.F. Long

    2011-07-01

    Gravel-dominated strata, inter-bedded with, and overlying coal-bearing units, are interpreted as deposits of wandering gravel-bed rivers, with sinuosity approaching 1.4. In most exposures they appear to be dominated by massive and thin planar-bedded granule to small pebble conglomerates, which would traditionally be interpreted as sheet-flood or longitudinal bar deposits of a high-gradient braided stream or alluvial fan. Architectural analysis of exposures in an open-pit shows that the predominance of flat bedding is an artefact of the geometry of the roadside exposures. In the pit the conglomerates are dominated by large scale cross stratification on a scale of 1–5.5 m. These appear to have developed as downstream and lateral accretion elements on side-bars and on in-channel bars in water depths of 2–12 m. Stacking of strata on domed 3rd order surfaces suggests development of longitudinal in-channel bar complexes similar to those observed in parts of the modern Rhône River system. Mudstone preserved in some of the channels reflects intervals of channel abandonment or avulsion. Minimum channel width is from 70 to 450 m.

  12. High-level defense waste solidification at the Savannah River Plant

    Energy Technology Data Exchange (ETDEWEB)

    Rhoad, H.D.

    1980-01-01

    Radioactive waste management at the Savannah River Plant is described. Their process for solidifying liquid wastes is discussed. Leaching studies of glass were performed and the results are discussed. (DC)

  13. Estimating subsurface water volumes and transit times in Hokkaido river catchments, Japan, using high-accuracy tritium analysis

    Science.gov (United States)

    Gusyev, Maksym; Yamazaki, Yusuke; Morgenstern, Uwe; Stewart, Mike; Kashiwaya, Kazuhisa; Hirai, Yasuyuki; Kuribayashi, Daisuke; Sawano, Hisaya

    2015-04-01

    The goal of this study is to estimate subsurface water transit times and volumes in headwater catchments of Hokkaido, Japan, using the New Zealand high-accuracy tritium analysis technique. Transit time provides insights into the subsurface water storage and therefore provides a robust and quick approach to quantifying the subsurface groundwater volume. Our method is based on tritium measurements in river water. Tritium is a component of meteoric water, decays with a half-life of 12.32 years, and is inert in the subsurface after the water enters the groundwater system. Therefore, tritium is ideally suited for characterization of the catchment's responses and can provide information on mean water transit times up to 200 years. Only in recent years has it become possible to use tritium for dating of stream and river water, due to the fading impact of the bomb-tritium from thermo-nuclear weapons testing, and due to improved measurement accuracy for the extremely low natural tritium concentrations. Transit time of the water discharge is one of the most crucial parameters for understanding the response of catchments and estimating subsurface water volume. While many tritium transit time studies have been conducted in New Zealand, only a limited number of tritium studies have been conducted in Japan. In addition, the meteorological, orographic and geological conditions of Hokkaido Island are similar to those in parts of New Zealand, allowing for comparison between these regions. In 2014, three field trips were conducted in Hokkaido in June, July and October to sample river water at river gauging stations operated by the Ministry of Land, Infrastructure, Transport and Tourism (MLIT). These stations have altitudes between 36 m and 860 m MSL and drainage areas between 45 and 377 km2. Each sampled point is located upstream of MLIT dams, with hourly measurements of precipitation and river water levels enabling us to distinguish between the snow melt and baseflow contributions

  14. High resolution seismic survey, Pen Branch Fault, Savannah River Site, South Carolina

    Energy Technology Data Exchange (ETDEWEB)

    Berkman, E. (Emerald Exploration Consultants, Inc., Austin, TX (United States))

    1991-04-01

    An investigation of the Pen Branch Fault at the Savannah River Site by a series of short, high resolution seismic reflection lines was conducted. The purpose was to acquire, process, and interpret 19.9 miles of data, optimized for the upper 300 ft of geologic strata, in sufficient density such that processing performed in the conventional stepwise approach, followed by detailed interpretation, would define small scale spatial variability and structural features in the vicinity of the fault leading to definition of the location of the fault, the shallowest extent of the fault, and the quantification of the sense and magnitude of motion. The depth of optimization for the last two lines was modified to the 300 ft of geologic strata immediately above basement. Three older seismic surveys, other geophysical data, and associated borehole and geologic data were reviewed. The equipment and the acquisition, processing, and interpretation procedures are discussed in the report. The report includes a detailed line by line description and discussion of the interpretation. Figures include reference maps, contour displays of the stacking and interval velocities, diagrammatic references sketches of the interpreted layering and sedimentary features, index sketches, and specific color prints made on the workstation during the course of the interpretation. A volume of manuals on seismic devices and related equipment is included.

  15. High resolution seismic survey, Pen Branch Fault, Savannah River Site, South Carolina. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Berkman, E. [Emerald Exploration Consultants, Inc., Austin, TX (United States)

    1991-04-01

    An investigation of the Pen Branch Fault at the Savannah River Site by a series of short, high resolution seismic reflection lines was conducted. The purpose was to acquire, process, and interpret 19.9 miles of data, optimized for the upper 300 ft of geologic strata, in sufficient density such that processing performed in the conventional stepwise approach, followed by detailed interpretation, would define small scale spatial variability and structural features in the vicinity of the fault leading to definition of the location of the fault, the shallowest extent of the fault, and the quantification of the sense and magnitude of motion. The depth of optimization for the last two lines was modified to the 300 ft of geologic strata immediately above basement. Three older seismic surveys, other geophysical data, and associated borehole and geologic data were reviewed. The equipment and the acquisition, processing, and interpretation procedures are discussed in the report. The report includes a detailed line by line description and discussion of the interpretation. Figures include reference maps, contour displays of the stacking and interval velocities, diagrammatic references sketches of the interpreted layering and sedimentary features, index sketches, and specific color prints made on the workstation during the course of the interpretation. A volume of manuals on seismic devices and related equipment is included.

  16. Summary Of Cold Crucible Vitrification Tests Results With Savannah River Site High Level Waste Surrogates

    Energy Technology Data Exchange (ETDEWEB)

    Stefanovsky, Sergey; Marra, James; Lebedev, Vladimir

    2014-01-13

    The cold crucible inductive melting (CCIM) technology successfully applied for vitrification of low- and intermediate-level waste (LILW) at SIA Radon, Russia, was tested to be implemented for vitrification of high-level waste (HLW) stored at Savannah River Site, USA. Mixtures of Sludge Batch 2 (SB2) and 4 (SB4) waste surrogates and borosilicate frits as slurries were vitrified in bench- (236 mm inner diameter) and full-scale (418 mm inner diameter) cold crucibles. Various process conditions were tested and major process variables were determined. Melts were poured into 10L canisters and cooled to room temperature in air or in heat-insulated boxes by a regime similar to Canister Centerline Cooling (CCC) used at DWPF. The products with waste loading from ~40 to ~65 wt.% were investigated in details. The products contained 40 to 55 wt.% waste oxides were predominantly amorphous; at higher waste loadings (WL) spinel structure phases and nepheline were present. Normalized release values for Li, B, Na, and Si determined by PCT procedure remain lower than those from EA glass at waste loadings of up to 60 wt.%.

  17. Summary Of Cold Crucible Vitrification Tests Results With Savannah River Site High Level Waste Surrogates

    Energy Technology Data Exchange (ETDEWEB)

    Stefanovsky, Sergey; Marra, James; Lebedev, Vladimir

    2014-01-13

    The cold crucible inductive melting (CCIM) technology successfully applied for vitrification of low- and intermediate-level waste (LILW) at SIA Radon, Russia, was tested to be implemented for vitrification of high-level waste (HLW) stored at Savannah River Site, USA. Mixtures of Sludge Batch 2 (SB2) and 4 (SB4) waste surrogates and borosilicate frits as slurries were vitrified in bench- (236 mm inner diameter) and full-scale (418 mm inner diameter) cold crucibles. Various process conditions were tested and major process variables were determined. Melts were poured into 10L canisters and cooled to room temperature in air or in heat-insulated boxes by a regime similar to Canister Centerline Cooling (CCC) used at DWPF. The products with waste loading from ~40 to ~65 wt.% were investigated in details. The products contained 40 to 55 wt.% waste oxides were predominantly amorphous; at higher waste loadings (WL) spinel structure phases and nepheline were present. Normalized release values for Li, B, Na, and Si determined by PCT procedure remain lower than those from EA glass at waste loadings of up to 60 wt.%.

  18. High prevalence of multiple-antibiotic-resistant (MAR) Escherichia coli in river bed sediments of the Apies River, South Africa

    CSIR Research Space (South Africa)

    Abia, ALK

    2015-10-01

    Full Text Available that sediments are a reservoir for bacteria and possibly other pathogens including antibiotic-resistant bacteria but also suggests that antibiotic-resistant genes could be transferred to pathogens due to the high prevalence of multiple-antibiotic-resistant (MAR...

  19. Effects of climate change on three flow regime-related ecosystem services in a highly-regulated Alpine river

    Science.gov (United States)

    Carolli, Mauro; Zolezzi, Guido; Geneletti, Davide; Majone, Bruno; Bellin, Alberto

    2016-04-01

    River systems provide several flow regime-related ecosystem services (ES) to society. The flow regime of several Alpine rivers is often regulated by hydropower production, which represents one of the most relevant ES in the area. Climate change is expected to modify the flow regime of rivers, with possible relevant consequences on the suitability of related ES. In this work we applied an approach aimed at evaluating the variations of ES under different flow regime conditions and consequently, the possibility to quantify the effects of different climate change scenarios on river ecosystem services. The case-study is the Noce River, a gravel-bed river in the Italian Alps (Trentino, North East Italy) which hydrological regime is subject to daily alterations of flow regime (hydropeaking) induced by the management of large hydropower plants. Here we considered three ES indicators: habitat for adult marble trout as representative for habitat provisioning service, rafting for recreational services, and small hydropower production as provisioning service. In particular, we evaluated the daily variations of these indicators under three different operating scenarios: a reference scenarios (REF, from 1970 to 2000) and two future scenarios (from 2040 to 2070), with (FUT) and without (FUT CC) the inclusion of the required minimum environmental flow (minimum vital flow) recently implemented in the regional water resources policy. For each scenario, four climate models have been applied (see Majone et al., 2016). Future scenarios indicate a modification of the flow regime, with a direct effect on the suitability of related ES. The effects on ES differ according with climate models and management scenarios: as a general result and considering the comparison with respect to the reference period, the applied models predict a temporal shift from late to early summer in the rafting suitability, a decrease of the suitability for trout in spring months and an increase of the suitability

  20. High homocysteine levels prevent via H2 S the CoCl2 -induced alteration of lymphocyte viability.

    Science.gov (United States)

    Bruzzese, Laurie; Fenouillet, Emmanuel; Fromonot, Julien; Durand-Gorde, Josée-Martine; Condo, Jocelyne; Kipson, Nathalie; Mottola, Giovanna; Deharo, Pierre; Guieu, Régis; Ruf, Jean

    2016-08-01

    High homocysteine (HCy) levels are associated with lymphocyte-mediated inflammatory responses that are sometimes in turn related to hypoxia. Because adenosine is a potent lymphocyte suppressor produced in hypoxic conditions and shares metabolic pathways with HCy, we addressed the influence of high HCy levels on the hypoxia-induced, adenosine-mediated, alteration of lymphocyte viability. We treated mitogen-stimulated human lymphocytes isolated from healthy individuals and the human lymphoma T-cell line CEM with cobalt chloride (CoCl2 )to reproduce hypoxia. We found that CoCl2 -altered cell viability was dose-dependently reversed using HCy. In turn, the HCy effect was inhibited using DL-propargylglycine, a specific inhibitor of the hydrogen sulphide (H2 S)-synthesizing enzyme cystathionine-γ-lyase involved in HCy catabolism. We then addressed the intracellular metabolic pathway of adenosine and HCy, and the role of the adenosine A2A receptor (A2 A R). We observed that: (i) hypoxic conditions lowered the intracellular concentration of HCy by increasing adenosine production, which resulted in high A2 A R expression and 3', 5'-cyclic adenosine monophosphate production; (ii) increasing intracellular HCy concentration reversed the hypoxia-induced adenosinergic signalling despite high adenosine concentration by promoting both S-adenosylhomocysteine and H2 S production; (iii) DL-propargylglycine that inhibits H2 S production abolished the HCy effect. Together, these data suggest that high HCy levels prevent, via H2 S production and the resulting down-regulation of A2 A R expression, the hypoxia-induced adenosinergic alteration of lymphocyte viability. We point out the relevance of these mechanisms in the pathophysiology of cardiovascular diseases.

  1. High-fidelity numerical modeling of the Upper Mississippi River under extreme flood condition

    Science.gov (United States)

    Khosronejad, Ali; Le, Trung; DeWall, Petra; Bartelt, Nicole; Woldeamlak, Solomon; Yang, Xiaolei; Sotiropoulos, Fotis

    2016-12-01

    We present data-driven numerical simulations of extreme flooding in a large-scale river coupling coherent-structure resolving hydrodynamics with bed morphodynamics under live-bed conditions. The study area is a ∼ 3.2 km long and ∼ 300 m wide reach of the Upper Mississippi River, near Minneapolis MN, which contains several natural islands and man-made hydraulic structures. We employ the large-eddy simulation (LES) and bed-morphodynamic modules of the Virtual Flow Simulator (VFS-Rivers) model, a recently developed in-house code, to investigate the flow and bed evolution of the river during a 100-year flood event. The coupling of the two modules is carried out via a fluid-structure interaction approach using a nested domain approach to enhance the resolution of bridge scour predictions. We integrate data from airborne Light Detection and Ranging (LiDAR), sub-aqueous sonar apparatus on-board a boat and in-situ laser scanners to construct a digital elevation model of the river bathymetry and surrounding flood plain, including islands and bridge piers. A field campaign under base-flow condition is also carried out to collect mean flow measurements via Acoustic Doppler Current Profiler (ADCP) to validate the hydrodynamic module of the VFS-Rivers model. Our simulation results for the bed evolution of the river under the 100-year flood reveal complex sediment transport dynamics near the bridge piers consisting of both scour and refilling events due to the continuous passage of sand dunes. We find that the scour depth near the bridge piers can reach to a maximum of ∼ 9 m. The data-driven simulation strategy we present in this work exemplifies a practical simulation-based-engineering-approach to investigate the resilience of infrastructures to extreme flood events in intricate field-scale riverine systems.

  2. Tidal Bore detection in the Garonne River using high frequency GNSS data

    Science.gov (United States)

    Frappart, Frédéric; Roussel, Nicolas; Darrozes, José; Bonneton, Philippe; Bonneton, Natalie; Detandt, Guillaume; Perosanz, Felix; Loyer, Sylvain

    2016-04-01

    A tidal bore is a positive surge propagating upstream that may form when a rising tide with significant amplitude enters shallow, gently sloping and narrowing rivers. Tidal bores have a significant impact on the river ecosystem behavior, especially in terms of sediment transport. Most of the existing field studies were limited to visual observations. Only a few field experiments have been devoted to a quantitative study of the tidal bore dynamics. We carried out a field study in August, 2015, using a GNSS buoy to measure the tidal bore in the Garonne River (France) at Podensac located 140 km upstream of the estuary mouth. Precise Point Positioning and Differential GNSS techniques were used to determine the river surface height variations with a 20 Hz sampling rate. This site was selected owing to the presence of well-developed undular tidal bores and also because of the absence of any significant curvature of the river at this location, which limits the complexity of the tidal bore structure. The Gironde estuary is located in the Bay of Biscay, on the southwest coast of France, and is formed from the meeting of the rivers Dordogne and Garonne. In the Gironde mouth, the mean neap tidal range and mean spring tidal range is 2.5 m and 5 m, respectively. As the tide propagates upstream a marked ebbflood asymmetry occurs in the upper reaches of the estuary and the wave is amplified. This large amplitude tidal wave propagates in the Garonne and Dordogne rivers up to 160 km from the estuary mouth. Both GNSS buoy and reference station use a Leica AR10 antenna and GR25 receiver. Both stations (reference and buoy) acquired data with a 20 Hz sampling rate. GNSS data were processed using RTKLib. Results allowed to detect the the wave train of the tidal bore that caused an elevation of the surface of around 1.5 m. Comparisons were performed using acoustic data showing a good agreement between both sources of data.

  3. Synoptic estimates of diffuse groundwater seepage to a spring-fed karst river at high spatial resolution using an automated radon measurement technique

    Science.gov (United States)

    Khadka, Mitra B.; Martin, Jonathan B.; Kurz, Marie J.

    2017-01-01

    Groundwater (GW) seepage can provide a major source of water, solutes, and contaminants to rivers, but identifying magnitudes, directions and locations of seepage is complicated by its diffuse and heterogeneous distributions. However, such information is necessary to develop programs and policies for protecting ecosystems and managing water resources. Here, we assess GW seepage to the Ichetucknee River, a spring-fed, low gradient, gaining stream in north-central Florida, through automated longitudinal surveys of radon (222Rn) activities at three different flow conditions. A 222Rn mass balance model, which integrates groundwater and spring water end member 222Rn activities and longitudinal 222Rn distributions in river water, shows that diffuse groundwater seepage represents about 16% of the total river baseflow, consistent with previous results obtained from ion (Ca2+, Cl-, SRP and Fe) mass balances and dye tracer methods. During high river stage, the contribution from seepage increases to 18-23% of the river flow. The spatial distribution of GW seepage is more variable in the upper 2.2-km reach of the river than the lower 2.8-km reach, regardless of river flow conditions. The upper reach has a narrower flood plain than the lower reach, which limits evapotranspiration and increases hydraulic gradients toward the river following storm events. Seepage in the lower reach is also limited by hydrologic damming by the receiving river, which inundates the floodplain during high flow conditions, and reduces the hydraulic head gradient. These results demonstrate the variable nature of seepage to a gaining river in both time and space and indicate that multiple synoptic analyses of GW seepage are required to assess seepage rates, determine time-averaged solute fluxes, and develop optimal management policies for riverine ecosystems.

  4. Vegetation cover, avoided erosion and water quality in high Andean wetlands, Yeso River Basin

    Science.gov (United States)

    León, Alejandro; Soto, Jorge; Seguel, Oscar; Pérez, Javier; Osses, Daniela; Leiva, Nicolás; Zerega, Linka

    2017-04-01

    Wetlands on the high Andes mountains near Santiago de Chile have been impacted by overgrazing and off-road tourists. We studied wetlands in El Yeso River basin. In February 2015 we established 36 exclusions and measured vegetation cover and height, biomass production in and out the exclusions starting in October. Water and undisturbed soil samples were collected. Data were analyzed statistically to estimate i) the recovery of vegetation, and ii) the influence of grazing and vehicle traffic on vegetation loss, and iii) impacts on soil and water quality. In areas with less intense traffic, the difference in vegetation coverage in and out the exclusions is 22% (± 11.4%); in areas with more intense traffic this difference is 16% (± 16%). Height of vegetation, in the less intense traffic areas, ranges from 6.25 cm (± 2.8) to 13.32 cm (± 6.3). With higher traffic it varies between 6.9 cm (± 3.1) and 13.6 cm (± 5.4). Biomass varies between 0.06 kg DM/m2 to 0.57 kg DM/m2 depending on botanical composition and date. After water circulates through the wetlands its content of nitrogen increases 37.33% to 0.37 mg N/l and the fecal coliforms 66.67% to 0.67 MPN/100 ml, because of cattle. On the contrary, turbidity decreases 20.67% to 0.21 UNT because sediments are captured by vegetation. We also estimated an avoided erosion rate, ranging between 1.23% and 31.87% (depending on the slope) due to the increase in coverage within the exclusions.

  5. Timing applications of growth regulators to alter spring cereal development at high latitudes

    Directory of Open Access Journals (Sweden)

    A. RAJALA

    2008-12-01

    Full Text Available Plant growth regulators (PGRs are commonly used in commercial farming to control lodging in cereals. PGRs have been shown to alter yield formation and plant stand structure, other than the straw length. To study their potential in Northern growing conditions PGRs and their application time impacts on plant stand structure and yield formation in tall and short statured cultivars of barley, oat, and wheat were studied in the field. Crop stands were sprayed with the gibberellin biosynthesis inhibitors CCC [chlormequat chloride CCC], Moddus [Trinexapac-ethyl TE], or with ethylene-releasing Cerone [ethephon ETH] at the recommended times or at an earlier growth stage. CCC applied at Zadoks growth scale (ZGS 13-14 increased and ETH applied at ZGS 39-40 reduced grain yield of oat by 370 kg ha-1 and 270 kg ha-1, respectively. In wheat, CCC applied at ZGS 31-32 reduced grain yield by 480 kg ha-1. This yield reduction was associated with lower grain yield production by the main head and particularly lower single grain weight. In barley cv. Kymppi, ETH and TE treatments promoted yield formation, whereas in cv. Saana they tended to reduce yield. Early applied PGRs reduced stem height at 14 days after treatment irrespective of species or stem stature, but at maturity no constant PGR effect was noted. Excluding the stem length, PGRs did not modify plant stand structure or yield formation markedly.

  6. Activity and High-Order Effective Connectivity Alterations in Sanfilippo C Patient-Specific Neuronal Networks

    Science.gov (United States)

    Canals, Isaac; Soriano, Jordi; Orlandi, Javier G.; Torrent, Roger; Richaud-Patin, Yvonne; Jiménez-Delgado, Senda; Merlin, Simone; Follenzi, Antonia; Consiglio, Antonella; Vilageliu, Lluïsa; Grinberg, Daniel; Raya, Angel

    2015-01-01

    Summary Induced pluripotent stem cell (iPSC) technology has been successfully used to recapitulate phenotypic traits of several human diseases in vitro. Patient-specific iPSC-based disease models are also expected to reveal early functional phenotypes, although this remains to be proved. Here, we generated iPSC lines from two patients with Sanfilippo type C syndrome, a lysosomal storage disorder with inheritable progressive neurodegeneration. Mature neurons obtained from patient-specific iPSC lines recapitulated the main known phenotypes of the disease, not present in genetically corrected patient-specific iPSC-derived cultures. Moreover, neuronal networks organized in vitro from mature patient-derived neurons showed early defects in neuronal activity, network-wide degradation, and altered effective connectivity. Our findings establish the importance of iPSC-based technology to identify early functional phenotypes, which can in turn shed light on the pathological mechanisms occurring in Sanfilippo syndrome. This technology also has the potential to provide valuable readouts to screen compounds, which can prevent the onset of neurodegeneration. PMID:26411903

  7. Activity and High-Order Effective Connectivity Alterations in Sanfilippo C Patient-Specific Neuronal Networks

    Directory of Open Access Journals (Sweden)

    Isaac Canals

    2015-10-01

    Full Text Available Induced pluripotent stem cell (iPSC technology has been successfully used to recapitulate phenotypic traits of several human diseases in vitro. Patient-specific iPSC-based disease models are also expected to reveal early functional phenotypes, although this remains to be proved. Here, we generated iPSC lines from two patients with Sanfilippo type C syndrome, a lysosomal storage disorder with inheritable progressive neurodegeneration. Mature neurons obtained from patient-specific iPSC lines recapitulated the main known phenotypes of the disease, not present in genetically corrected patient-specific iPSC-derived cultures. Moreover, neuronal networks organized in vitro from mature patient-derived neurons showed early defects in neuronal activity, network-wide degradation, and altered effective connectivity. Our findings establish the importance of iPSC-based technology to identify early functional phenotypes, which can in turn shed light on the pathological mechanisms occurring in Sanfilippo syndrome. This technology also has the potential to provide valuable readouts to screen compounds, which can prevent the onset of neurodegeneration.

  8. Activity and High-Order Effective Connectivity Alterations in Sanfilippo C Patient-Specific Neuronal Networks.

    Science.gov (United States)

    Canals, Isaac; Soriano, Jordi; Orlandi, Javier G; Torrent, Roger; Richaud-Patin, Yvonne; Jiménez-Delgado, Senda; Merlin, Simone; Follenzi, Antonia; Consiglio, Antonella; Vilageliu, Lluïsa; Grinberg, Daniel; Raya, Angel

    2015-10-13

    Induced pluripotent stem cell (iPSC) technology has been successfully used to recapitulate phenotypic traits of several human diseases in vitro. Patient-specific iPSC-based disease models are also expected to reveal early functional phenotypes, although this remains to be proved. Here, we generated iPSC lines from two patients with Sanfilippo type C syndrome, a lysosomal storage disorder with inheritable progressive neurodegeneration. Mature neurons obtained from patient-specific iPSC lines recapitulated the main known phenotypes of the disease, not present in genetically corrected patient-specific iPSC-derived cultures. Moreover, neuronal networks organized in vitro from mature patient-derived neurons showed early defects in neuronal activity, network-wide degradation, and altered effective connectivity. Our findings establish the importance of iPSC-based technology to identify early functional phenotypes, which can in turn shed light on the pathological mechanisms occurring in Sanfilippo syndrome. This technology also has the potential to provide valuable readouts to screen compounds, which can prevent the onset of neurodegeneration.

  9. Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model

    Science.gov (United States)

    Honorato-Sampaio, Kinulpe; Rossoni Júnior, Joamyr Victor; Andrade Leal, Diego; Pinto, Angélica Barbosa G.; Kappes-Becker, Lenice; Evangelista, Elisio Alberto; Guerra-Sá, Renata

    2017-01-01

    Endurance exercise is a remarkable intervention for the treatment of many diseases. Mitochondrial changes on skeletal muscle are likely important for many of the benefits provided by exercise. In this study, we aimed to evaluate the effects that a regular physical activity (swimming without workload) has on mitochondrial morphological alterations and glucometabolic parameters induced by a high-sugar diet (HSD). Weaned male Wistar rats fed with a standard diet or a HSD (68% carbohydrate) were subjected to 60 minutes of regular physical activity by swimming (without workload) for four- (20 sessions) or eight-week (40 sessions) periods. After training, animals were euthanized and the sera, adipose tissues, and skeletal muscles were collected for further analysis. The HSD increased body weight after an 8-week period; it also increased the fat pads and the adipose index, resulting in glucose intolerance and insulin resistance (IR). Transmission electron microscopy showed an increase in alterations of mitochondrial ultrastructure in the gastrocnemius muscle, as well as a decrease in superoxide dismutase (SOD) activity, and an increase in protein carbonylation. Regular physical activity partially reverted these alterations in rats fed a HSD, preventing mitochondrial morphological alterations and IR. Moreover, we observed a decrease in Pgc1α expression (qPCR analysis) in STD-EXE group and a less pronounced reduction in HSD-EXE group after an 8-week period. Thus, regular physical activity (swimming without workload) in rats fed a HSD can prevent mitochondrial dysfunction and IR, highlighting the crucial role for physical activity on metabolic homeostasis. PMID:28199417

  10. Cytochrome p450 induction and gonadal status alteration in common carp (Cyprinus carpio) associated with the discharge of dioxin contaminated effluent to the Hikiji River, Kanagawa Prefecture, Japan.

    Science.gov (United States)

    Sakamoto, Kentaro Q; Nakai, Kiyotaka; Aoto, Takahiro; Yokoyama, Aiko; Ushikoshi, Ryoko; Hirose, Hitomi; Ishizuka, Mayumi; Kazusaka, Akio; Fujita, Shoichi

    2003-05-01

    Accumulations of polychlorinated dibenzo-p-dioxins, dibenzofurans and coplanar polychlorinated biphenyls were analyzed in common carp (Cyprinus carpio) collected in the Hikiji River, Kanagawa Prefecture, Japan in which dioxin contaminated effluent was released during the period starting from November 1992 to March 2000. Higher levels of 2,3,7,8-tetrachlorinated dibenzo-p-dioxin toxic equivalents were observed in carps collected downstream to the dioxin release site (contaminated site) than the reference site. Modulations of cytochrome p450 (CYP) enzyme in liver, serum estrogen concentration and gonadal somatic index (GSI) were also measured as biomarkers for the contaminants. Total CYP content in livers was markedly higher in male and female carps from the contaminated site relative to the reference site fish. The expression level of the cytochrome p450 1A and Ethoxyresorufin O-deethylase activity were significantly higher in female carps from the contaminated site than from the reference site. A lower level of plasma estrogen was observed in carps from the contaminated site. The GSI in female carps from the contaminated site was smaller than that recorded at the reference site. The present study indicates that dioxins released to the Hikiji River might induce the CYP enzyme and inhibit the reproductive functions in common carps dwelling downstream from the release site.

  11. Temporal Variations in the Roughness of Eroding River Banks Revealed by High-Resolution Digital Photogrammetry and Terrestrial Laser Scanning

    Science.gov (United States)

    Darby, S. E.; Leyland, J.; Rinaldi, M.; Teruggi, L.; Ostuni, D.

    2010-12-01

    of the roughness is approximated as a series of user defined Gaussian functions, with the skin drag component characterised by the deviation of points from the fitted curves. In our study these bank ‘roughness profiles’ are extracted from an annual series (2003-present) of high-resolution DEMs of the river bank, the latter being constructed either through digital photogrammetry or terrestrial laser scanning surveys. The DEMs are used to quantify accurately the spatial trends and amounts of annual bank erosion observed in relation to the hydrological regime of the river, and are compared to the temporal variations in river bank form and skin roughness components as the bank erodes. The data are used to evaluate the extent to which there is a dynamic feedback between the bank erosion process and bank form roughness.

  12. A river-scale Lagrangian experiment examining controls on phytoplankton dynamics in the presence and absence of treated wastewater effluent high in ammonium

    Science.gov (United States)

    Kraus, Tamara; Carpenter, Kurt; Bergamaschi, Brian; Parker, Alexander; Stumpner, Elizabeth; Downing, Bryan D.; Travis, Nicole; Wilkerson, Frances; Kendall, Carol; Mussen, Timothy

    2017-01-01

    Phytoplankton are critical component of the food web in most large rivers and estuaries, and thus identifying dominant controls on phytoplankton abundance and species composition is important to scientists, managers, and policymakers. Recent studies from a variety of systems indicate that ammonium ( NH+4) in treated wastewater effluent decreases primary production and alters phytoplankton species composition. However, these findings are based mainly on laboratory and enclosure studies, which may not adequately represent natural systems. To test effects of effluent high in ammonium on phytoplankton at the ecosystem scale, we conducted whole-river–scale experiments by halting discharges to the Sacramento River from the regional wastewater treatment plant (WWTP), and used a Lagrangian approach to compare changes in phytoplankton abundance and species composition in the presence (+EFF) and absence (−EFF) of effluent. Over 5 d of downstream travel from 20 km above to 50 km below the WWTP, chlorophyll concentrations declined from 15–25 to ∼2.5 μg L−1, irrespective of effluent addition. Benthic diatoms were dominant in most samples. We found no significant difference in phytoplankton abundance or species composition between +EFF and −EFF conditions. Moreover, greatest declines in chlorophyll occurred upstream of the WWTP where NH+4 concentrations were low. Grazing by clams and zooplankton could not account for observed losses, suggesting other factors such as hydrodynamics and light limitation were responsible for phytoplankton declines. These results highlight the advantages of conducting ecosystem-scale, Lagrangian-based experiments to understand the dynamic and complex interplay between physical, chemical, and biological factors that control phytoplankton populations.

  13. Highly efficient generation of transgenic sheep by lentivirus accompanying the alteration of methylation status.

    Directory of Open Access Journals (Sweden)

    Chenxi Liu

    Full Text Available BACKGROUND: Low efficiency of gene transfer and silence of transgene expression are the critical factors hampering the development of transgenic livestock. Recently, transfer of recombinant lentivirus has been demonstrated to be an efficient transgene delivery method in various animals. However, the lentiviral transgenesis and the methylation status of transgene in sheep have not been well addressed. METHODOLOGY/PRINCIPLE FINDINGS: EGFP transgenic sheep were generated by injecting recombinant lentivirus into zygotes. Of the 13 lambs born, 8 carried the EGFP transgene, and its chromosomal integration was identified in all tested tissues. Western blotting showed that GFP was expressed in all transgenic founders and their various tissues. Analysis of CpG methylation status of CMV promoter by bisulfate sequencing unraveled remarkable variation of methylation levels in transgenic sheep. The average methylation levels ranged from 37.6% to 79.1% in the transgenic individuals and 34.7% to 83% in the tested tissues. Correlative analysis of methylation status with GFP expression revealed that the GFP expression level was inversely correlated with methylation density. The similar phenomenon was also observed in tested tissues. Transgene integration determined by Southern blotting presented multiple integrants ranging from 2 to 6 copies in the genome of transgenic sheep. CONCLUSIONS/SIGNIFICANCE: Injection of lentiviral transgene into zygotes could be a promising efficient gene delivery system to generate transgenic sheep and achieved widespread transgene expression. The promoter of integrants transferred by lentiviral vector was subjected to dramatic alteration of methylation status and the transgene expression level was inversely correlative with promoter methylation density. Our work illustrated for the first time that generation of transgenic sheep by injecting recombinant lentivirus into zygote could be an efficient tool to improve sheep performance by

  14. Lower implantation rates in high responders: evidence for an altered endocrine milieu during the preimplantation period.

    Science.gov (United States)

    Pellicer, A; Valbuena, D; Cano, F; Remohí, J; Simón, C

    1996-06-01

    To determine serum E2 and P levels around the time of implantation in normal and high IVF responders. In Vitro Fertilization program at the Instituto Valenciano de Infertilidad. Twenty-nine women undergoing IVF, who accepted to be studied daily, were classified according to the number of oocytes retrieved in normal (n = 16) and high responders (n = 13). Prospective study in which blood was drawn daily from the day of hCG administration (day 0) up to 7 days later (day 6). In vitro fertilization parameters (number of ampules, FSH-hMG, number of oocytes, fertilization rates, number of transferred embryos, implantation rates, and pregnancy rates); serum E2 and P levels during the 7 days of the study. Implantation rate was significantly higher in normal (18.5%) as compared with high (0%) responders. Estradiol and P levels were elevated significantly in high responders. The E2:P ratio was significantly different between normal and high responders during the preimplantation period. Pregnancy and implantation rates decreased as serum E2 levels increased on days 4 to 6 of the study. A different endocrine milieu between normal and high responders is detected by daily steroid measurements up to the preimplantation period, suggesting that this difference could be responsible for an impaired implantation in high responder patients undergoing IVF. An increase in serum E2 levels seems to be the cause of this difference.

  15. High Variability Is a Defining Component of Mediterranean-Climate Rivers and Their Biota

    Directory of Open Access Journals (Sweden)

    Núria Cid

    2017-01-01

    Full Text Available Variability in flow as a result of seasonal precipitation patterns is a defining element of streams and rivers in Mediterranean-climate regions of the world and strongly influences the biota of these unique systems. Mediterranean-climate areas include the Mediterranean Basin and parts of Australia, California, Chile, and South Africa. Mediterranean streams and rivers can experience wet winters and consequent floods to severe droughts, when intermittency in otherwise perennial systems can occur. Inter-annual variation in precipitation can include multi-year droughts or consecutive wet years. Spatial variation in patterns of precipitation (rain vs. snow combined with topographic variability lead to spatial variability in hydrologic patterns that influence populations and communities. Mediterranean streams and rivers are global biodiversity hotspots and are particularly vulnerable to human impacts. Biomonitoring, conservation efforts, and management responses to climate change require approaches that account for spatial and temporal variability (including both intra- and inter-annual. The importance of long-term data sets for understanding and managing these systems highlights the need for sustained and coordinated research efforts in Mediterranean-climate streams and rivers.

  16. Distinct laterality alterations distinguish mild cognitive impairment and Alzheimer's disease from healthy aging: statistical parametric mapping with high resolution MRI.

    Science.gov (United States)

    Long, Xiaojing; Zhang, Lijuan; Liao, Weiqi; Jiang, Chunxiang; Qiu, Bensheng

    2013-12-01

    Laterality of human brain varies under healthy aging and diseased conditions. The alterations in hemispheric asymmetry may embed distinct biomarkers linked to the disease dynamics. Statistical parametric mapping based on high-resolution magnetic resonance imaging (MRI) and image processing techniques have allowed automated characterization of morphological features across the entire brain. In this study, 149 subjects grouped in healthy young, healthy elderly, mild cognitive impairment (MCI), and Alzheimer's disease (AD) were investigated using multivariate analysis for regional cerebral laterality indexed by surface area, curvature index, cortical thickness, and subjacent white matter volume measured on high-resolution MR images. Asymmetry alteration of MCI and AD were characterized by marked region-specific reduction, while healthy elderly featured a distinct laterality shift in the limbic system in addition to regional asymmetry loss. Lack of the laterality shift in limbic system and early loss of asymmetry in entorhinal cortex may be biomarkers to identify preclinical AD among other dementia. Multivariate analysis of hemispheric asymmetry may provide information helpful for monitoring the disease progress and improving the management of MCI and AD.

  17. Low carbohydrate/high-fat diet attenuates cardiac hypertrophy, remodeling, and altered gene expression in hypertension

    Science.gov (United States)

    The effects of dietary fat intake on the development of left ventricular hypertrophy and accompanying structural and molecular remodeling in response to hypertension are not understood. The present study compared the effects of a high-fat versus a low-fat diet on development of left ventricular hype...

  18. High- cholesterol diet does not alter gut microbiota composition in mice

    NARCIS (Netherlands)

    Dimova, Lidiya G.; Zlatkov, Nikola; Verkade, Henkjan J.; Uhlin, Bernt Eric; Tietge, Uwe J. F.

    2017-01-01

    Introduction: Western diet containing both saturated fat and cholesterol impairs cardio- metabolic health partly by modulating diversity and function of the microbiota. While diet containing only high fat has comparable effects, it is unclear how diets only enriched in cholesterol impact the

  19. Design high water level of Suzhou River%苏州河沿线设计高水位

    Institute of Scientific and Technical Information of China (English)

    徐贵泉; 陈庆江; 陈长太

    2012-01-01

    In order to determine the crest elevation of flood-prevention wall rehabilitation along Suzhou River, by means of the combination of hydrological probability statistics and hydraulic test, mathematical model and prototype observation, a hydrological and hydrodynamic model for Suzhou River system based on digital river networks is established. It is integrated with the hydrological and hydrodynamic model for Taihu Lake basin. The factors for the high water level of Suzhou River are analyzed, including floods, waterlog, tides and control. The distribution of the high water levels of Suzhou River under the unitary condition of floods, waterlog and tides as well as the typical historical hydrological record is calculated and analyzed. The results show that the design high water levels under the current, short-term and long-term planning conditions are 4. 60 m, 4. 79 m and 4. 77 m, respectively.%为确定苏州河两岸防汛墙改造的墙顶高程,采用水文概率统计与水力模型计算相结合、原型实测与数学模型相结合的方法,与太湖流域河网水文水动力模型相衔接,建立基于数字河网的苏州河水系水文水动力模型,从影响苏州河高水位的洪、涝、潮、控等要素着手,计算分析洪、涝、潮单一水情以及历史典型年不利水情影响下的苏州河高水位分布规律.研究结果表明,现状及规划近、远期工况条件下的苏州河沿线设计高水位分别为4.60m,4.79m和4.77m.

  20. DEMONSTRATION AND EVALUATION OF POTENTIAL HIGH LEVEL WASTE MELTER DECONTAMINATION TECHNOLOGIES FOR SAVANNAH RIVER SITE

    Energy Technology Data Exchange (ETDEWEB)

    Weger, Hans, Ph.D.; Kodanda, Raja Tilek Meruva; Mazumdar, Anindra; Srivastava, Rajiv Ph.D.; Ebadian, M.A. Ph.D.

    2003-02-27

    Four hand-held tools were tested for failed high-level waste melter decontamination and decommissioning (D&D). The forces felt by the tools during operation were measured using a tri-axial accelerometer since they will be operated by a remote manipulator. The efficiency of the tools was also recorded. Melter D&D consists of three parts: (1) glass fracturing: removing from the furnace the melted glass that can not be poured out through normal means, (2) glass cleaning: removing the thin layer of glass that has formed over the surface of the refractory material, and (3) K-3 refractory breakup: removing the K-3 refractory material. Surrogate glass, from a formula provided by the Savannah River Site, was melted in a furnace and poured into steel containers. K-3 refractory material, the same material used in the Defense Waste Processing Facility, was utilized for the demonstrations. Four K-3 blocks were heated at 1150 C for two weeks with a glass layer on top to simulate the hardened glass layer on the refractory surface in the melter. Tools chosen for the demonstrations were commonly used D&D tools, which have not been tested specifically for the different aspects of melter D&D. A jackhammer and a needle gun were tested for glass fracturing; a needle gun and a rotary grinder with a diamond face wheel (diamond grinder) were tested for glass cleaning; and a jackhammer, diamond grinder, and a circular saw with a diamond blade were tested for refractory breakup. The needle gun was not capable of removing or fracturing the surrogate glass. The diamond grinder only had a removal rate of 3.0 x 10-4 kg/s for K-3 refractory breakup and needed to be held firmly against the material. However, the diamond grinder was effective for glass cleaning, with a removal rate of 3.9 cm2/s. The jackhammer was successful in fracturing glass and breaking up the K-3 refractory block. The jackhammer had a glass-fracturing rate of 0.40 kg/s. The jackhammer split the K-3 refractory block into two

  1. Circulating adiponectin concentration and body composition are altered in response to high-intensity interval training.

    Science.gov (United States)

    Shing, Cecilia M; Webb, Jessica J; Driller, Matthew W; Williams, Andrew D; Fell, James W

    2013-08-01

    Adiponectin influences metabolic adaptations that would prove beneficial to endurance athletes, and yet to date there is little known about the response of adiponectin concentrations to exercise, and, in particular, the response of this hormone to training in an athlete population. This study aimed to determine the response of plasma adiponectin concentrations to acute exercise after 2 different training programs and to determine the influence of the training on body composition. Seven state-level representative rowers (age: 19 ± 1.2 years [mean ± SD], height: 1.77 ± 0.10 m, body mass: 74.0 ± 10.7 kg, VO2peak 62.1 ± 7.0 ml·kg·min) participated in the double-blind, randomized crossover investigation. Rowers performed an incremental graded exercise test before and after completing 4 weeks of high-intensity interval ergometer training and 4 weeks of traditional ergometer rowing training. Rowers' body composition was assessed at baseline and after each training program. Significant increases in plasma adiponectin concentration occurred in response to maximal exercise after completion of the high-intensity interval training (p = 0.016) but not after traditional ergometer rowing training (p = 0.69). The high-intensity interval training also resulted in significant increases in mean 4-minute power output (p = 0.002) and VO2peak (p = 0.05), and a decrease in body fat percentage (p = 0.022). Mean 4-minute power output, VO2peak, and body fat percentage were not significantly different after 4 weeks of traditional ergometer rowing training (p > 0.05). Four weeks of high-intensity interval training is associated with an increase in adiponectin concentration in response to maximal exercise and a reduction in body fat percentage. The potential for changes in adiponectin concentration to reflect positive training adaptations and athlete performance level should be further explored.

  2. High temperatures alter physiological properties of pyramidal cells and inhibitory interneurons in hippocampus

    OpenAIRE

    Kim, Jennifer A; Barry W Connors

    2012-01-01

    Temperature has multiple effects on neurons, yet little is known about the effects of high temperature on the physiology of mammalian central neurons. Hyperthermia can influence behavior and cause febrile seizures. We studied the effects of acute hyperthermia on the immature hippocampus in vitro by recording from pyramidal neurons and inhibitory oriens-lacunosum moleculare (O-LM) interneurons (identified by green fluorescent protein (GFP) expression in the GIN mouse line). Warming to 41°C cau...

  3. Alterations in DNA methylation and genome structure in two rice mutant lines induced by high pressure

    Institute of Scientific and Technical Information of China (English)

    SHEN; Sile; WANG; Zhenwei; SHAN; Xiaohui; WANG; Hua; LI; Ling; LIN; Xuyun; LONG; Likun; WENG; Kenan; LIU; Bao; ZOU; Guangtian

    2006-01-01

    By using high-pressure treatment, two mutant lines were obtained from a genetically stable japonica rice cultivar Bijing38. Genomic DNA of the mutant lines, together with the original line (Bijing38), was either undigested or digested by Hpa II/Msp I, and then subjected to molecular analysis using two markers, ISSR and RAPD. Results indicated that changes in the PCR amplification profiles of both markers are apparent in the two mutant lines compared with the original rice cultivar, suggesting that there had been both sequence changes and DNA methylation modifications in the mutant lines. Southern blot analysis using diverse sequences, including two cellular genes (S2 and S3), a set of retrotransposons (Osr7, Osr36, Tos19 and more), and a MITE transposon family (mPing and Pong), confirmed the results, and indicated that changes in DNA methylation pattern, genomic structure, and possible activation of some transposons indeed occurred in the mutant lines. Moreover, these changes are stably maintained through selfed generations and in different organs. Thus, our results indicate that it is possible to obtain stable mutants in rice by high pressure treatments, and the molecular basis of the mutants may include both genetic and epigenetic changes. Therefore, high hydrostatic pressure seems a promising approach for plant mutagenesis.

  4. Influences of anthropogenic activities and topography on water quality in the highly regulated Huai River basin, China.

    Science.gov (United States)

    Shi, Wei; Xia, Jun; Zhang, Xiang

    2016-11-01

    Our study analyzed the spatio-temporal trends of four major water quality parameters (i.e., dissolved oxygen (DO), ammonium nitrogen (NH3-N), total phosphorus (TP) and permanganate index (CODMn)) at 17 monitoring stations in one of the most polluted large river basins, Huai River Basin, in China during 2005 to 2014. More concerns were emphasized on the attributions, e.g., anthropogenic actives (land cover, pollution load, water temperature, and regulated flow) and natural factors (topography) to the changes in the water quality. The seasonal Mann-Kendall test indicated that water quality conditions were significantly improved during the study period. The results given by the Moran's I methods demonstrated that NH3-N and CODMn existed a weak and moderate positive spatial autocorrelation. Two cluster centers of significant high concentrations can be detected for DO and TP at the Mengcheng and Huaidian station, respectively, while four cluster centers of significant low concentrations for DO at Wangjiaba and Huaidian station in the 2010s. Multiple linear regression analysis suggested that water temperature, regulated flow, and load of water quality could significantly influence the water quality variations. Additionally, urban land cover was the primary predictor for NH3-N and CODMn at large scale. The predictive ability of regression models for NH3-N and CODMn declined as the scale decreases or the period ranges from the 2000s to the 2010s. Topography variables of elevation and slope, which can be treated as the important explanatory variables, exhibited positive and negative correlations to NH3-N and CODMn, respectively. This research can help us identify the water quality variations from the scale-process interactions and provide a scientific basis for comprehensive water quality management and decision making in the Huai River Basin and also other river basins over the world.

  5. Dynamic Feedbacks Between Flow, Erosion and Evolving River Bank Roughness Revealed Through Repeat High-Resolution Topographic Surveys

    Science.gov (United States)

    Leyland, J.; Darby, S. E.; Rinaldi, M.; Teruggi, L. B.; Ostuni, D.

    2012-12-01

    Bank erosion is a key process in fluvial dynamics, with significant fractions of the total sediment load being sourced from river banks. Studies have shown that hydraulic erosion of the bank toe is a driving factor of long term rates of bank retreat. Fluvial bank erosion rates are often quantified using an excess shear stress model where the erosion rate is a function of the boundary shear stress applied by the flow above a critical threshold. Research has shown that the form roughness induced by natural topographic bank features such as slumps, spurs and embayments, is a major component of the spatially-averaged total shear stress. The skin friction component of this shear stress is typically an order of magnitude less than the total, meaning that the form roughness provides an important control on bank erosion rates. However, measuring the relative components of the total shear stress for a natural system is not straightforward. In this research we apply the method of Kean and Smith [2006, J. Geophys. Res., 111(4), F04009, doi:10.1029/2006JF000467] to partition the form and skin drag components of river bank roughness for an eroding bank of the Cecina River in central Italy. This method approximates the form drag component of the roughness along a longitudinal bank profile as a series of user defined Gaussian curves, with the skin friction component estimated through analysis of the deviations of the data from the fitted curves. For our site, a temporal sequence (2003 - 2011) of high-resolution topographic surveys has been collected through a combination of photogrammetry and Terrestrial Laser Scanning. For each survey five vertically equidistant profiles are extracted and analysed alongside DEMs of difference and associated flow data modelled using the distributed hydrological model MOBIDIC. The data are used to explore the dynamic feedbacks that exist between river discharge, bank erosion processes and bank form roughness, revealing insights into the self

  6. High glucose induced alteration of SIRTs in endothelial cells causes rapid aging in a p300 and FOXO regulated pathway.

    Directory of Open Access Journals (Sweden)

    Rokhsana Mortuza

    Full Text Available In diabetes, some of the cellular changes are similar to aging. We hypothesized that hyperglycemia accelerates aging-like changes in the endothelial cells (ECs and tissues leading to structural and functional damage. We investigated glucose-induced aging in 3 types of ECs using senescence associated β-gal (SA β-gal staining and cell morphology. Alterations of sirtuins (SIRTs and their downstream mediator FOXO and oxidative stress were investigated. Relationship of such alteration with histone acetylase (HAT p300 was examined. Similar examinations were performed in tissues of diabetic animals. ECs in high glucose (HG showed evidence of early senescence as demonstrated by increased SA β-gal positivity and reduced replicative capacities. These alterations were pronounced in microvascular ECs. They developed an irregular and hypertrophic phenotype. Such changes were associated with decreased SIRT (1-7 mRNA expressions. We also found that p300 and SIRT1 regulate each other in such process, as silencing one led to increase of the others' expression. Furthermore, HG caused reduction in FOXO1's DNA binding ability and antioxidant target gene expressions. Chemically induced increased SIRT1 activity and p300 knockdown corrected these abnormalities slowing aging-like changes. Diabetic animals showed increased cellular senescence in renal glomerulus and retinal blood vessels along with reduced SIRT1 mRNA expressions in these tissues. Data from this study demonstrated that hyperglycemia accelerates aging-like process in the vascular ECs and such process is mediated via downregulation of SIRT1, causing reduction of mitochondrial antioxidant enzyme in a p300 and FOXO1 mediated pathway.

  7. High Dietary Folate in Mice Alters Immune Response and Reduces Survival after Malarial Infection.

    Directory of Open Access Journals (Sweden)

    Danielle N Meadows

    Full Text Available Malaria is a significant global health issue, with nearly 200 million cases in 2013 alone. Parasites obtain folate from the host or synthesize it de novo. Folate consumption has increased in many populations, prompting concerns regarding potential deleterious consequences of higher intake. The impact of high dietary folate on the host's immune function and response to malaria has not been examined. Our goal was to determine whether high dietary folate would affect response to malarial infection in a murine model of cerebral malaria. Mice were fed control diets (CD, recommended folate level for rodents or folic acid-supplemented diets (FASD, 10x recommended level for 5 weeks before infection with Plasmodium berghei ANKA. Survival, parasitemia, numbers of immune cells and other infection parameters were assessed. FASD mice had reduced survival (p<0.01, Cox proportional hazards and higher parasitemia (p< 0.01, joint model of parasitemia and survival compared with CD mice. FASD mice had lower numbers of splenocytes, total T cells, and lower numbers of specific T and NK cell sub-populations, compared with CD mice (p<0.05, linear mixed effects. Increased brain TNFα immunoreactive protein (p<0.01, t-test and increased liver Abca1 mRNA (p<0.01, t-test, a modulator of TNFα, were observed in FASD mice; these variables correlated positively (rs = 0.63, p = 0.01. Bcl-xl/Bak mRNA was increased in liver of FASD mice (p<0.01, t-test, suggesting reduced apoptotic potential. We conclude that high dietary folate increases parasite replication, disturbs the immune response and reduces resistance to malaria in mice. These findings have relevance for malaria-endemic regions, when considering anti-folate anti-malarials, food fortification or vitamin supplementation programs.

  8. Preparation and properties of SYNROC D containing simulated Savannah River Plant high-level defense waste

    Energy Technology Data Exchange (ETDEWEB)

    Hoenig, C.; Rozsa, R.; Bazan, F.; Otto, R.; Grens, J.

    1981-07-23

    We describe in detail the formulation and processing steps used to prepare all SYNROC D samples tested in the Comparative Leach Testing Program at the Savannah River Laboratory. We also discuss how the composition of the Savannah River Plant sludge influences the formulation and ultimate preparation of SYNROC D. Mechanical properties are reported in the categories of elastic constants, flexural and compressive strengths, and microhardness; thermal expansion and thermal conductivity results are presented. The thermal expansion data indicated the presence of significant residual strain and the possibility of an unidentified amorphous or glassy phase in the microstructure. We summarize the standardized (MCC) leaching results for both crushed Synroc and monoliths in deionized water, silicate water, and salt brine at 90/sup 0/C and 150/sup 0/C.

  9. Impaired suppression of plasma free fatty acids and triglycerides by acute hyperglycaemia-induced hyperinsulinaemia and alterations in high density lipoproteins in essential hypertension

    NARCIS (Netherlands)

    Ligtenberg, JJM; vanTol, A; vanHaeften, TW; Sluiter, WJ; Dullaart, RPF

    1996-01-01

    Objectives. Essential hypertension may be associated with abnormalities in free fatty acids (FFA) and triglyceride metabolism, which could lead to alterations in high density lipoproteins (HDL). Lecithin: cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP) are key factor

  10. Impaired suppression of plasma free fatty acids and triglycerides by acute hyperglycaemia-induced hyperinsulinaemia and alterations in high density lipoproteins in essential hypertension

    NARCIS (Netherlands)

    Ligtenberg, JJM; vanTol, A; vanHaeften, TW; Sluiter, WJ; Dullaart, RPF

    1996-01-01

    Objectives. Essential hypertension may be associated with abnormalities in free fatty acids (FFA) and triglyceride metabolism, which could lead to alterations in high density lipoproteins (HDL). Lecithin: cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP) are key

  11. Pressure-induced alteration in effects of high CO2 on marine bacteria

    Science.gov (United States)

    Yamada, N.; Tsukasaki, A.; Tsurushima, N.; Suzumura, M.

    2013-12-01

    Carbon capture and storage (CCS) is a key mitigation technology to reduce the release of carbon dioxide (CO2) into the atmosphere. Current CCS research is dominated by improvements of the efficiency of the capturing, transport or storage of CO2. Also, it is important to estimate potential impacts on marine environments related to potential CO2 leakage. It has been demonstrated that seawater acidification effects on marine community structure and food chains. Bacteria are the basis of marine microbial food web and responsible for a significant part of marine biogeochemical cycles in both water column and bottom sediments. We used a high pressure incubation system which is composed of an HPLC pump and stainless-steel pressure vessels. The system could maintain stably the pressure up to 30 MPa. Using the system, we investigated the effects of high CO2 concentration on a deep-sea bacterium, Pseudoalteromonas sp., isolated from the western North Pacific Ocean. The isolate was incubated in acidified seawaters at various CO2 concentrations under simulated pressure conditions between 0.1 MPa and 30 MPa. We determined bacterial growth rate and live/dead cell viability. It was found that both CO2 concentration and pressure influenced substantially the growth rate of the isolate. In order to assess potential effects of leaked CO2 on microbial assemblages in marine environments, it was suggested that hydraulic pressure is one essential variable to be considered.

  12. Alterations in the upper facial growth of Macaca mulatta resulting from high-pull headgear.

    Science.gov (United States)

    Meldrum, R J

    1975-04-01

    Four prepubertal Macaca mulatta monkeys, ranging in age from 13 to 24 months, were used in an investigation of the effects of high-pull headgear (to a face-bow) therapy on the growth of the upper facial skeleton. Amalgam bone implants were placed across the frontomaxillary, frontozygomatic, zygomaticomaxillary, and zygomaticotemporal sutures in each animal. Three of the monkeys wore appliances consisting of a maxillary dental spling, a face-bow, two coil springs, and an acrylic helmet. The fourth monkey (control) wore only a dental splint and a face-bow. A continuous high-pull headgear force of 300 grams per side was applied to the three monkeys for 81, 87 and 89 days, respectively, before death. Procion brilliant red 8-HBS vital stain was administered to all four animals at the start of and 3 days before the end of the treatment period. The facial growth patterns were determined from lateral cephalograms taken before and after treatment, from direct measurement of implant separation at the sutures, and from histologic sections of the four mentioned facial sutures.

  13. High fat diet produces brain insulin resistance, synaptodendritic abnormalities and altered behavior in mice.

    Science.gov (United States)

    Arnold, Steven E; Lucki, Irwin; Brookshire, Bethany R; Carlson, Gregory C; Browne, Caroline A; Kazi, Hala; Bang, Sookhee; Choi, Bo-Ran; Chen, Yong; McMullen, Mary F; Kim, Sangwon F

    2014-07-01

    Insulin resistance and other features of the metabolic syndrome are increasingly recognized for their effects on cognitive health. To ascertain mechanisms by which this occurs, we fed mice a very high fat diet (60% kcal by fat) for 17days or a moderate high fat diet (HFD, 45% kcal by fat) for 8weeks and examined changes in brain insulin signaling responses, hippocampal synaptodendritic protein expression, and spatial working memory. Compared to normal control diet mice, cerebral cortex tissues of HFD mice were insulin-resistant as evidenced by failed activation of Akt, S6 and GSK3β with ex-vivo insulin stimulation. Importantly, we found that expression of brain IPMK, which is necessary for mTOR/Akt signaling, remained decreased in HFD mice upon activation of AMPK. HFD mouse hippocampus exhibited increased expression of serine-phosphorylated insulin receptor substrate 1 (IRS1-pS(616)), a marker of insulin resistance, as well as decreased expression of PSD-95, a scaffolding protein enriched in post-synaptic densities, and synaptopodin, an actin-associated protein enriched in spine apparatuses. Spatial working memory was impaired as assessed by decreased spontaneous alternation in a T-maze. These findings indicate that HFD is associated with telencephalic insulin resistance and deleterious effects on synaptic integrity and cognitive behaviors.

  14. Amazon river dolphins (Inia geoffrensis) use a high-frequency short-range biosonar

    OpenAIRE

    Michael Ledegaard; Frants Havmand Jensen; Mafalda de Freitas; Vera Maria Ferreira da Silva; Peter Teglberg Madsen

    2015-01-01

    Toothed whales produce echolocation clicks with source parameters related to body size; however, it may be equally important to consider the influence of habitat, as suggested by studies on echolocating bats. A few toothed whale species have fully adapted to river systems, where sonar operation is likely to result in higher clutter and reverberation levels than those experienced by most toothed whales at sea because of the shallow water and dense vegetation. To test the hypothesis that habita...

  15. High-fat diet and glucocorticoid treatment cause hyperglycemia associated with adiponectin receptor alterations

    Directory of Open Access Journals (Sweden)

    Oller do Nascimento Cláudia

    2011-01-01

    Full Text Available Abstract Background Adiponectin is the most abundant plasma protein synthesized for the most part in adipose tissue, and it is an insulin-sensitive hormone, playing a central role in glucose and lipid metabolism. In addition, it increases fatty acid oxidation in the muscle and potentiates insulin inhibition of hepatic gluconeogenesis. Two adiponectin receptors have been identified: AdipoR1 is the major receptor expressed in skeletal muscle, whereas AdipoR2 is mainly expressed in liver. Consumption of high levels of dietary fat is thought to be a major factor in the promotion of obesity and insulin resistance. Excessive levels of cortisol are characterized by the symptoms of abdominal obesity, hypertension, glucose intolerance or diabetes and dyslipidemia; of note, all of these features are shared by the condition of insulin resistance. Although it has been shown that glucocorticoids inhibit adiponectin expression in vitro and in vivo, little is known about the regulation of adiponectin receptors. The link between glucocorticoids and insulin resistance may involve the adiponectin receptors and adrenalectomy might play a role not only in regulate expression and secretion of adiponectin, as well regulate the respective receptors in several tissues. Results Feeding of a high-fat diet increased serum glucose levels and decreased adiponectin and adipoR2 mRNA expression in subcutaneous and retroperitoneal adipose tissues, respectively. Moreover, it increased both adipoR1 and adipoR2 mRNA levels in muscle and adipoR2 protein levels in liver. Adrenalectomy combined with the synthetic glucocorticoid dexamethasone treatment resulted in increased glucose and insulin levels, decreased serum adiponectin levels, reduced adiponectin mRNA in epididymal adipose tissue, reduction of adipoR2 mRNA by 7-fold in muscle and reduced adipoR1 and adipoR2 protein levels in muscle. Adrenalectomy alone increased adiponectin mRNA expression 3-fold in subcutaneous adipose

  16. High-fat diet transition reduces brain DHA levels associated with altered brain plasticity and behaviour.

    Science.gov (United States)

    Sharma, Sandeep; Zhuang, Yumei; Gomez-Pinilla, Fernando

    2012-01-01

    To assess how the shift from a healthy diet rich in omega-3 fatty acids to a diet rich in saturated fatty acid affects the substrates for brain plasticity and function, we used pregnant rats fed with omega-3 supplemented diet from their 2nd day of gestation period as well as their male pups for 12 weeks. Afterwards, the animals were randomly assigned to either a group fed on the same diet or a group fed on a high-fat diet (HFD) rich in saturated fats for 3 weeks. We found that the HFD increased vulnerability for anxiety-like behavior, and that these modifications harmonized with changes in the anxiety-related NPY1 receptor and the reduced levels of BDNF, and its signalling receptor pTrkB, as well as the CREB protein. Brain DHA contents were significantly associated with the levels of anxiety-like behavior in these rats.

  17. Deeper snow alters soil nutrient availability and leaf nutrient status in high Arctic tundra

    DEFF Research Database (Denmark)

    Semenchuk, Philipp R.; Elberling, Bo; Amtorp, Cecilie

    2015-01-01

    Nitrogen (N) mineralization, nutrient availability, and plant growth in the Arctic are often restricted by low temperatures. Predicted increases of cold-season temperatures may be important for plant nutrient availability and growth, given that N mineralization is also taking place during the cold...... season. Changing nutrient availability may be reflected in plant N and chlorophyll content and lead to increased photosynthetic capacity, plant growth, and ultimately carbon (C) assimilation by plants. In this study, we increased snow depth and thereby cold-season soil temperatures in high Arctic...... for some species. Responses to cold-season soil warming are vegetation type- and species-specific, with potentially stronger responses in moister vegetation types. This study therefore highlights the contrasting effect of snow in a tundra landscape and has important implications for projections of whole...

  18. Calorie anticipation alters food intake after low-caloric not high-caloric preloads.

    Science.gov (United States)

    Hogenkamp, P S; Cedernaes, J; Chapman, C D; Vogel, H; Hjorth, O C; Zarei, S; Lundberg, L S; Brooks, S J; Dickson, S L; Benedict, C; Schiöth, H B

    2013-08-01

    Cognitive factors and anticipation are known to influence food intake. The current study examined the effect of anticipation and actual consumption of food on hormone (ghrelin, cortisol, and insulin) and glucose levels, appetite and ad libitum intake, to assess whether changes in hormone levels might explain the predicted differences in subsequent food intake. During four breakfast sessions, participants consumed a yogurt preload that was either low caloric (LC: 180 kcal/300 g) or high caloric (HC: 530 kcal/300 g) and was provided with either consistent or inconsistent calorie information (i.e., stating the caloric content of the preload was low or high). Appetite ratings and hormone and glucose levels were measured at baseline (t = 0), after providing the calorie information about the preload (t = 20), after consumption of the preload (t = 40), and just before ad libitum intake (t = 60). Ad libitum intake was lower after HC preloads (as compared to LC preloads; P Intake after LC preloads was higher when provided with (consistent) LC information (467±254 kcal) as compared to (inconsistent) HC information (346±210 kcal), but intake after the HC preloads did not depend on the information provided (LC information: 290±178 kcal, HC information: 333±179 kcal; caloric load*information P = 0.03). Hormone levels did not respond in an anticipatory manner, and the post-prandial responses depended on actual calories consumed. These results suggest that both cognitive and physiological information determine food intake. When actual caloric intake was sufficient to produce physiological satiety, cognitive factors played no role; however, when physiological satiety was limited, cognitively induced satiety reduced intake to comparable levels. Copyright © 2012 The Obesity Society.

  19. Bacteriophage lambda terminase: alterations of the high-affinity ATPase affect viral DNA packaging.

    Science.gov (United States)

    Dhar, Alok; Feiss, Michael

    2005-03-18

    DNA packaging by large DNA viruses such as the tailed bacteriophages and the herpesviruses involves DNA translocation into a preformed protein shell, called the prohead. Translocation is driven by an ATP hydrolysis-powered DNA packaging motor. The bacteriophages encode a heterodimeric viral DNA packaging protein, called terminase. The terminases have an ATPase center located in the N terminus of the large subunit implicated in DNA translocation. In previous work with phage lambda, lethal mutations that changed ATP-reactive residues 46 and 84 of gpA, the large terminase subunit, were studied. These mutant enzymes retained the terminase endonuclease and helicase activities, but had severe defects in virion assembly, and lacked the terminase high-affinity ATPase activity. Surprisingly, in the work described here, we found that enzymes with the conservative gpA changes Y46F and Y46A had only mild packaging defects. These mild defects contrast with their profound virion assembly defects. Thus, these mutant enzymes have, in addition to the mild DNA packaging defects, a severe post-DNA packaging defect. In contrast, the gpA K84A enzyme had similar virion assembly and DNA packaging defects. The DNA packaging energy budget, i.e. DNA packaged/ATP hydrolyzed, was unchanged for the mutant enzymes, indicating that DNA translocation is tightly coupled to ATP hydrolysis. A model is proposed in which gpA residues 46 and 84 are important for terminase's high-affinity ATPase activity. Assembly of the translocation complex remodels this ATPase so that residues 46 and 84 are not crucial for the activated translocation ATPase. Changing gpA residues 46 and 84 primarily affects assembly, rather than the activity, of the translocation complex.

  20. Capsaicin-induced transcriptional changes in hypothalamus and alterations in gut microbial count in high fat diet fed mice.

    Science.gov (United States)

    Baboota, Ritesh K; Murtaza, Nida; Jagtap, Sneha; Singh, Dhirendra P; Karmase, Aniket; Kaur, Jaspreet; Bhutani, Kamlesh K; Boparai, Ravneet K; Premkumar, Louis S; Kondepudi, Kanthi Kiran; Bishnoi, Mahendra

    2014-09-01

    Obesity is a global health problem and recently it has been seen as a growing concern for developing countries. Several bioactive dietary molecules have been associated with amelioration of obesity and associated complications and capsaicin is one among them. The present work is an attempt to understand and provide evidence for the novel mechanisms of anti-obesity activity of capsaicin in high fat diet (HFD)-fed mice. Swiss albino mice divided in three groups (n=8-10) i.e. control, HFD fed and capsaicin (2mg/kg, po)+HFD fed were administered respective treatment for 3months. After measuring phenotypic and serum related biochemical changes, effect of capsaicin on HFD-induced transcriptional changes in hypothalamus, white adipose tissue (WAT) (visceral and subcutaneous), brown adipose tissue (BAT) and gut microbial alterations was studied and quantified. Our results suggest that, in addition to its well-known effects, oral administration of capsaicin (a) modulates hypothalamic satiety associated genotype, (b) alters gut microbial composition, (c) induces "browning" genotype (BAT associated genes) in subcutaneous WAT and (d) increases expression of thermogenesis and mitochondrial biogenesis genes in BAT. The present study provides evidence for novel and interesting mechanisms to explain the anti-obesity effect of capsaicin.

  1. Obesity alters immune and metabolic profiles: New insight from obese-resistant mice on high-fat diet.

    Science.gov (United States)

    Boi, Shannon K; Buchta, Claire M; Pearson, Nicole A; Francis, Meghan B; Meyerholz, David K; Grobe, Justin L; Norian, Lyse A

    2016-10-01

    Diet-induced obesity has been shown to alter immune function in mice, but distinguishing the effects of obesity from changes in diet composition is complicated. It was hypothesized that immunological differences would exist between diet-induced obese (DIO) and obese-resistant (OB-Res) mice fed the same high-fat diet (HFD). BALB/c mice were fed either standard chow or HFD to generate lean or DIO and OB-Res mice, respectively. Resulting mice were analyzed for serum immunologic and metabolic profiles and cellular immune parameters. BALB/c mice on HFD were categorized as DIO or OB-Res, based on body weight versus lean controls. DIO mice were physiologically distinct from OB-Res mice, whose serum insulin, leptin, gastric inhibitory polypeptide, and eotaxin concentrations remained similar to lean controls. DIO mice had increased macrophage(+) crown-like structures in white adipose tissue, although macrophage percentages were unchanged from OB-Res and lean mice. DIO mice also had decreased splenic CD4(+) T cells, elevated serum GM-CSF, and increased splenic CD11c(+) dendritic cells, but impaired dendritic cell stimulatory capacity (P Diet-induced obesity results in alterations in immune and metabolic profiles that are distinct from effects caused by HFD alone. © 2016 The Obesity Society.

  2. Altered resting-state connectivity in subjects at ultra-high risk for psychosis: an fMRI study

    Directory of Open Access Journals (Sweden)

    Choi Jung-Seok

    2010-10-01

    Full Text Available Abstract Background Individuals at ultra-high risk (UHR for psychosis have self-disturbances and deficits in social cognition and functioning. Midline default network areas, including the medial prefrontal cortex and posterior cingulate cortex, are implicated in self-referential and social cognitive tasks. Thus, the neural substrates within the default mode network (DMN have the potential to mediate self-referential and social cognitive information processing in UHR subjects. Methods This study utilized functional magnetic resonance imaging (fMRI to investigate resting-state DMN and task-related network (TRN functional connectivity in 19 UHR subjects and 20 matched healthy controls. The bilateral posterior cingulate cortex was selected as a seed region, and the intrinsic organization for all subjects was reconstructed on the basis of fMRI time series correlation. Results Default mode areas included the posterior/anterior cingulate cortices, the medial prefrontal cortex, the lateral parietal cortex, and the inferior temporal region. Task-related network areas included the dorsolateral prefrontal cortex, supplementary motor area, the inferior parietal lobule, and middle temporal cortex. Compared to healthy controls, UHR subjects exhibit hyperconnectivity within the default network regions and reduced anti-correlations (or negative correlations nearer to zero between the posterior cingulate cortex and task-related areas. Conclusions These findings suggest that abnormal resting-state network activity may be related with the clinical features of UHR subjects. Neurodevelopmental and anatomical alterations of cortical midline structure might underlie altered intrinsic networks in UHR subjects.

  3. Influences of high-flow events on a stream channel altered by construction of a highway bridge: A case study

    Science.gov (United States)

    Hedrick, Lara B.; Welsh, Stuart A.; Anderson, James T.

    2009-01-01

    Impacts of highway construction on streams in the central Appalachians are a growing concern as new roads are created to promote tourism and economic development in the area. Alterations to the streambed of a first-order stream, Sauerkraut Run, Hardy County, WV, during construction of a highway overpass included placement and removal of a temporary culvert, straightening and regrading of a section of stream channel, and armourment of a bank with a reinforced gravel berm. We surveyed longitudinal profiles and cross sections in a reference reach and the altered reach of Sauerkraut Run from 2003 through 2007 to measure physical changes in the streambed. During the four-year period, three high-flow events changed the streambed downstream of construction including channel widening and aggradation and then degradation of the streambed. Upstream of construction, at a reinforced gravel berm, bank erosion was documented. The reference section remained relatively unchanged. Knowledge gained by documenting channel changes in response to natural and anthropogenic variables can be useful for managers and engineers involved in highway construction projects.

  4. Dynamic alterations of serotonergic metabolism and receptors during social isolation of low- and high-active mice.

    Science.gov (United States)

    Rilke, O; Freier, D; Jähkel, M; Oehler, J

    1998-04-01

    Alterations induced by social isolation (1 day to 18 weeks) in low- and high-active mice (LAM and HAM) were studied in respect to serotonin metabolism, [3H]-8-OH-DPAT binding of presynaptic (midbrain), postsynaptic (hippocampus) 5-HT1A receptors and [3H]-ketanserin binding of cortical 5-HT2A receptors. Individual housing of mice was associated with reduction of serotonin metabolism, depending on isolation time and brain structure. Whereas a transient decrease in the striatum and cortex was detected between 1 week and 6 weeks, reduction of cerebellar and hippocampal serotonin metabolism was found later (12-18 weeks). Serotonergic systems of HAM were found to be more reactive to environmental disturbances, and their serotonin metabolism was more affected by social isolation. Isolation-induced upregulation of cortical 5-HT2A receptors was measured only in HAM. Densities of postsynaptic 5-HT1A receptors in the hippocampus did differ either in grouped or isolated mice. However, there were significant differences in hippocampal 5-HT1A receptor affinity, especially between 1 day and 3 weeks. Transient downregulation of presynaptic 5-HT1A receptors in the midbrain was found in isolated mice between 3 and 6 weeks. These results are discussed in terms of interactions between serotonergic alterations and isolation-induced aggression.

  5. High altitude increases alteration in maximal torque but not in rapid torque development in knee extensors after repeated treadmill sprinting

    Directory of Open Access Journals (Sweden)

    Olivier eGIRARD

    2016-03-01

    Full Text Available We assessed knee extensor neuromuscular adjustments following repeated treadmill sprints in different normobaric hypoxia conditions, with special reference to rapid muscle torque production capacity. Thirteen team- and racquet-sport athletes undertook 8 x 5-s all-out sprints (passive recovery = 25 s on a non-motorized treadmill in normoxia (NM; FiO2 = 20.9%, at low (LA; FiO2 = 16.6% and high (HA; FiO2 = 13.3% normobaric hypoxia (simulated altitudes of ~1800 m and ~3600 m, respectively. Explosive (∼1 s; fast instruction and maximal (∼5 s; hard instruction voluntary isometric contractions (MVC of the knee extensors, with concurrent electromyographic (EMG activity recordings of the vastus lateralis (VL and rectus femoris (RF muscles, were performed before and 1-min post-exercise. Rate of torque development (RTD and EMG (i.e., Root Mean Square or RMS rise from 0 to 30, -50, -100 and -200 ms were recorded, and were also normalized to maximal torque and EMG values, respectively. Distance covered during the first 5-s sprint was similar (P>0.05 in all conditions. A larger (P0.05. Irrespectively of condition (P>0.05, peak RTD (-6±11%; P0.05, whereas it increased (P<0.05 for RF muscle during all epochs post-exercise, independently of the conditions. In summary, alteration in repeated-sprint ability and post-exercise MVC decrease were greater at high altitude than in normoxia or at low altitude. However, the post-exercise alterations in RTD were similar between normoxia and low-to-high hypoxia.

  6. Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats.

    Science.gov (United States)

    Tinkov, Alexey A; Gatiatulina, Eugenia R; Popova, Elizaveta V; Polyakova, Valentina S; Skalnaya, Anastasia A; Agletdinov, Eduard F; Nikonorov, Alexandr A; Skalny, Anatoly V

    2017-02-01

    The primary objective of the current study was to assess the influence of early high-fat feeding on tissue trace element content in young male Wistar rats. Twenty weanling male Wistar rats were divided into two groups fed standard (STD) or high-fat diet (HFD) containing 10 and 31.6 % of total calories from fat, respectively, for 1 month. Serum lipid spectrum, apolipoproteins, glucose, insulin, adiponectin, and leptin levels were assessed. The level of trace elements was estimated using inductively coupled plasma mass spectrometry. High-fat feeding significantly increased epidydimal (EDAT) and retroperitoneal adipose tissue (RPAT), as well as total adipose tissue mass by 34, 103, and 59 %, respectively. Serum leptin levels in HFD animals were twofold higher than those in the control rats. No significant difference in serum lipid spectrum, apolipoproteins, glucose, adiponectin, and insulin was detected between the groups. HFD significantly altered tissue trace element content. In particular, HFD-fed animals were characterized by significantly lower levels of Cu, I, Mn, Se, and Zn in the liver; Cr, V, Co, Cu, Fe, and I content of EDAT; Co, Cu, I, Cr, V, Fe, and Zn concentration in RPAT samples. At the same time, only serum Cu was significantly depressed in HFD-fed animals as compared to the control ones. Hair Co, Mn, Si, and V levels were significantly increased in comparison to the control values, whereas Se and I content was decreased. HFD feeding induced excessive adiposity and altered tissue trace element content in rats without insulin resistance, adiponectin deficiency, and proatherogenic state. Hypothetically, trace element disbalance may precede obesity-associated metabolic disturbances.

  7. High and low protein∶ carbohydrate dietary ratios during gestation alter maternal-fetal cortisol regulation in pigs.

    Directory of Open Access Journals (Sweden)

    Ellen Kanitz

    Full Text Available Imbalanced maternal nutrition during gestation can cause alterations of the hypothalamic-pituitary-adrenal (HPA system in offspring. The present study investigated the effects of maternal low- and high-protein diets during gestation in pigs on the maternal-fetal HPA regulation and expression of the glucocorticoid receptor (GR, mineralocorticoid receptor (MR, 11β-hydroxysteroid dehydrogenase 1 and 2 (11β-HSD1 and 11β-HSD2 and c-fos mRNAs in the placenta and fetal brain. Twenty-seven German Landrace sows were fed diets with high (HP, 30%, low (LP, 6.5% or adequate (AP, 12.1% protein levels made isoenergetic by varying the carbohydrate levels. On gestational day 94, fetuses were recovered under general anesthesia for the collection of blood, brain and placenta samples. The LP diet in sows increased salivary cortisol levels during gestation compared to the HP and AP sows and caused an increase of placental GR and c-fos mRNA expression. However, the diurnal rhythm of plasma cortisol was disturbed in both LP and HP sows. Total plasma cortisol concentrations in the umbilical cord vessels were elevated in fetuses from HP sows, whereas corticosteroid-binding globulin levels were decreased in LP fetuses. In the hypothalamus, LP fetuses displayed an enhanced mRNA expression of 11β-HSD1 and a reduced expression of c-fos. Additionally, the 11β-HSD2 mRNA expression was decreased in both LP and HP fetuses. The present results suggest that both low and high protein∶carbohydrate dietary ratios during gestation may alter the expression of genes encoding key determinants of glucocorticoid hormone action in the fetus with potential long-lasting consequences for stress adaptation and health.

  8. Modelling algae growth and dissolved oxygen in the Seine River downstream the Paris urban area: contribution of high frequency measurements

    Science.gov (United States)

    Vilmin, Lauriane; Escoffier, Nicolas; Groleau, Alexis; Poulin, Michel; Flipo, Nicolas

    2014-05-01

    Dissolved oxygen is a key variable in the hydro-ecological functioning of river systems. The accurate representation of the different biogeochemical processes affecting algal blooms and dissolved oxygen in the water column in hydro-ecological models is crucial for the use of these models as reliable management tools. This study focuses on the water quality of the Seine River along a 225 km stretch, from Paris to the Seine estuary. The study area is highly urbanized and located downstream France's largest agricultural area, and therefore receives large amounts of nutrients. During the last decades, nutrient inputs have been significantly reduced, especially with the implementation of new sewage water treatment technologies. Even though the frequency and the intensity of observed algal blooms have decreased, blooms were observed in 2011 and 2012. These blooms are generally followed by a period of high organic matter accumulation, leading to high mineralization fluxes and potential oxygen depletion. The hydrodynamics and the water quality of the Seine River are simulated for the 2011-2012 period with the distributed process-based hydro-ecological model ProSe (Even et al., 1998). The simulated chlorophyll a and dissolved oxygen concentrations are compared to high frequency measurements at the Bougival monitoring station (50 km downstream from Paris), which is part of the CarboSeine monitoring network. The high frequency continuous dataset allows calibrating of primary producers' physiological parameters. New growth parameters are defined for the diatom community. The blooms occur at the end of the winter period (march 2011 and march 2012) and the optimal temperature for diatom growth is calibrated at 10°C, based on an analysis of the physiological response of the diatom community. One of the main outcomes of the modelling exercise is that the precise identification of the constituting communities of algal blooms must be achieved prior to the modelling itself. With the

  9. Magmatic-vapor expansion and the formation of high-sulfidation gold deposits: Chemical controls on alteration and mineralization

    Science.gov (United States)

    Henley, R.W.; Berger, B.R.

    2011-01-01

    Large bulk-tonnage high-sulfidation gold deposits, such as Yanacocha, Peru, are the surface expression of structurally-controlled lode gold deposits, such as El Indio, Chile. Both formed in active andesite-dacite volcanic terranes. Fluid inclusion, stable isotope and geologic data show that lode deposits formed within 1500. m of the paleo-surface as a consequence of the expansion of low-salinity, low-density magmatic vapor with very limited, if any, groundwater mixing. They are characterized by an initial 'Sulfate' Stage of advanced argillic wallrock alteration ?? alunite commonly with intense silicification followed by a 'Sulfide' Stage - a succession of discrete sulfide-sulfosalt veins that may be ore grade in gold and silver. Fluid inclusions in quartz formed during wallrock alteration have homogenization temperatures between 100 and over 500 ??C and preserve a record of a vapor-rich environment. Recent data for El Indio and similar deposits show that at the commencement of the Sulfide Stage, 'condensation' of Cu-As-S sulfosalt melts with trace concentrations of Sb, Te, Bi, Ag and Au occurred at > 600 ??C following pyrite deposition. Euhedral quartz crystals were simultaneously deposited from the vapor phase during crystallization of the vapor-saturated melt occurs to Fe-tennantite with progressive non-equilibrium fractionation of heavy metals between melt-vapor and solid. Vugs containing a range of sulfides, sulfosalts and gold record the changing composition of the vapor. Published fluid inclusion and mineralogical data are reviewed in the context of geological relationships to establish boundary conditions through which to trace the expansion of magmatic vapor from source to surface and consequent alteration and mineralization. Initially heat loss from the vapor is high resulting in the formation of acid condensate permeating through the wallrock. This Sulfate Stage alteration effectively isolates the expansion of magmatic vapor in subsurface fracture arrays

  10. Effect of burn-up and high burn-up structure on spent nuclear fuel alteration

    Energy Technology Data Exchange (ETDEWEB)

    Clarens, F.; Gonzalez-Robles, E.; Gimenez, F. J.; Casas, I.; Pablo, J. de; Serrano, D.; Wegen, D.; Glatz, J. P.; Martinez-Esparza, A.

    2009-07-01

    In this report the results of the experimental work carried out within the collaboration project between ITU-ENRESA-UPC/CTM on spent fuel (SF) covering the period 2005-2007 were presented. Studies on both RN release (Fast Release Fraction and matrix dissolution rate) and secondary phase formation were carried out by static and flow through experiments. Experiments were focussed on the study of the effect of BU with two PWR SF irradiated in commercial reactors with mean burn-ups of 48 and 60 MWd/KgU and; the effect of High Burn-up Structure (HBS) using powdered samples prepared from different radial positions. Additionally, two synthetic leaching solutions, bicarbonate and granitic bentonite ground wa ter were used. Higher releases were determined for RN from SF samples prepared from the center in comparison with the fuel from the periphery. However, within the studied range, no BU effect was observed. After one year of contact time, secondary phases were observed in batch experiments, covering the SF surface. Part of the work was performed for the Project NF-PRO of the European Commission 6th Framework Programme under contract no 2389. (Author)

  11. Mineral identification and mapping of hydrothermal alteration zones using high-spectral resolution images (AVIRIS)

    Science.gov (United States)

    Van Der Meer, Freek D.

    1994-01-01

    High-spectral resolution images (AVIRIS) of the cuprite mining area were used to evaluate atmospheric calibration algorithms and test several mineral mapping techniques. Four scene normalization techniques were used: (1) the flat-field method, (2) the internal average reflectance method, (3) the empirical line method, and (4) the atmospheric absorption removal method (ATREM). The algorithms were evaluated in terms of their spectral interpret- ability and their ability to remove both solar irradiance and atmospheric absorption features, noise, and artifacts. Noise was quantified by calculating the coefficient of variation of the spectra, and spectral interpretability was quantified by calcu- lating a difference spectrum (eg, laboratory spectrum minus pixel spectrum) for areas with known occurrences of clay minerals. These difference spectra were useful in evaluating the degree of removal of atmospheric features. The empirical line method produced the best calibration results. Mineral mapping as done using (1) color-composites of bands on the shoulders and centers of expected absorption features, (2) color-coded spectra, and (3) spectral angle mapping.

  12. Cellular and molecular alterations in human epithelial cells transformed by high let radiation

    Science.gov (United States)

    Hei, T. K.; Piao, C. Q.; Sutter, T.; Willey, J. C.; Suzuki, K.

    An understanding of the radiobiological effects of high LET radiation is essential for human risk estimation and radiation protection. In the present study, we show that a single, 30 cGy dose of 150 keV/mum ^4He ions can malignantly transform human papillomavirus immortalized human bronchial epithelial [BEP2D] cells. Transformed cells produce progressively growing tumors in nude mice. The transformation frequency by the single dose of alpha particles is estimated to be approximately 4 x 10^-7. Based on the average cross-sectional area of BEP2D cells, it can be calculated that a mean traversal of 1.4 particles per cell is sufficient to induce tumorigenic conversion of these cells 3 to 4 months post-irradiation. Tumorigenic BEP2D cells overexpress mutated p53 tumor suppressor oncoproteins in addition to the cell cycle control gene cyclin D1 and D2. This model provides an opportunity to study the cellular and molecular changes at the various stages in radiation carcinogenesis involving human cells.

  13. High-fructose corn syrup-55 consumption alters hepatic lipid metabolism and promotes triglyceride accumulation.

    Science.gov (United States)

    Mock, Kaitlin; Lateef, Sundus; Benedito, Vagner A; Tou, Janet C

    2017-01-01

    High-fructose corn syrup-55 (HFCS-55) has been suggested to be more lipogenic than sucrose, which increases the risk for nonalcoholic fatty liver disease (NAFLD) and dyslipidemia. The study objectives were to determine the effects of drinking different sugar-sweetened solutions on hepatic gene expression in relation to liver fatty acid composition and risk of NAFLD. Female rats were randomly assigned (n=7 rats/group) to drink water or water sweetened with 13% (w/v) HFCS-55, sucrose or fructose for 8 weeks. Rats drinking HFCS-55 solution had the highest (P=.03) hepatic total lipid and triglyceride content and histological evidence of fat infiltration. Rats drinking HFCS-55 solution had the highest hepatic de novo lipogenesis indicated by the up-regulation of stearoyl-CoA desaturase-1 and the highest (P55 solution. The observed lipogenic effects were attributed to the slightly higher fructose content of HFCS-55 solution in the absence of differences in macronutrient and total caloric intake between rats drinking HFCS-55 and sucrose solution. Results from gene expression and fatty acid composition analysis showed that, in a hypercaloric state, some types of sugars are more detrimental to the liver. Based on these preclinical study results, excess consumption of caloric sweetened beverage, particularly HFCS-sweetened beverages, should be limited. Published by Elsevier Inc.

  14. High-throughput sequencing reveals an altered T cell repertoire in X-linked agammaglobulinemia.

    Science.gov (United States)

    Ramesh, Manish; Simchoni, Noa; Hamm, David; Cunningham-Rundles, Charlotte

    2015-12-01

    To examine the T cell receptor structure in the absence of B cells, the TCR β CDR3 was sequenced from DNA of 15 X-linked agammaglobulinemia (XLA) subjects and 18 male controls, using the Illumina HiSeq platform and the ImmunoSEQ analyzer. V gene usage and the V-J combinations, derived from both productive and non-productive sequences, were significantly different between XLA samples and controls. Although the CDR3 length was similar for XLA and control samples, the CDR3 region of the XLA T cell receptor contained significantly fewer deletions and insertions in V, D, and J gene segments, differences intrinsic to the V(D)J recombination process and not due to peripheral T cell selection. XLA CDR3s demonstrated fewer charged amino acid residues, more sharing of CDR3 sequences, and almost completely lacked a population of highly modified Vβ gene segments found in control DNA, suggesting both a skewed and contracted T cell repertoire in XLA.

  15. Genomic Integration of High-Risk HPV Alters Gene Expression in Oropharyngeal Squamous Cell Carcinoma.

    Science.gov (United States)

    Walline, Heather M; Komarck, Christine M; McHugh, Jonathan B; Bellile, Emily L; Brenner, J Chad; Prince, Mark E; McKean, Erin L; Chepeha, Douglas B; Wolf, Gregory T; Worden, Francis P; Bradford, Carol R; Carey, Thomas E

    2016-10-01

    High-risk HPV (hrHPV) is the leading etiologic factor in oropharyngeal cancer. HPV-positive oropharyngeal tumors generally respond well to therapy, with complete recovery in approximately 80% of patients. However, it remains unclear why some patients are nonresponsive to treatment, with 20% of patients recurring within 5 years. In this study, viral factors were examined for possible clues to differences in tumor behavior. Oropharynx tumors that responded well to therapy were compared with those that persisted and recurred. Viral oncogene alternate transcripts were assessed, and cellular sites of viral integration were mapped and sequenced. Effects of integration on gene expression were assessed by transcript analysis at the integration sites. All of the tumors demonstrated active viral oncogenesis, indicated by expression of HPV E6 and E7 oncogenes and alternate E6 splicing. In the responsive tumors, HPV integration occurred exclusively in intergenic chromosome regions, except for one tumor with viral integration into TP63. Each recurrent tumor exhibited complex HPV integration patterns into cancer-associated genes, including TNFRSF13B, SCN2A, SH2B1, UBE2V2, SMOC1, NFIA, and SEMA6D Disrupted cellular transcripts were identified in the region of integration in four of the seven affected genes.

  16. Mineral identification and mapping of hydrothermal alteration zones using high-spectral resolution images (AVIRIS)

    Science.gov (United States)

    Van Der Meer, Freek D.

    1994-01-01

    High-spectral resolution images (AVIRIS) of the cuprite mining area were used to evaluate atmospheric calibration algorithms and test several mineral mapping techniques. Four scene normalization techniques were used: (1) the flat-field method, (2) the internal average reflectance method, (3) the empirical line method, and (4) the atmospheric absorption removal method (ATREM). The algorithms were evaluated in terms of their spectral interpret- ability and their ability to remove both solar irradiance and atmospheric absorption features, noise, and artifacts. Noise was quantified by calculating the coefficient of variation of the spectra, and spectral interpretability was quantified by calcu- lating a difference spectrum (eg, laboratory spectrum minus pixel spectrum) for areas with known occurrences of clay minerals. These difference spectra were useful in evaluating the degree of removal of atmospheric features. The empirical line method produced the best calibration results. Mineral mapping as done using (1) color-composites of bands on the shoulders and centers of expected absorption features, (2) color-coded spectra, and (3) spectral angle mapping.

  17. Alteration of gas phase ion polarizabilities upon hydration in high dielectric liquids.

    Science.gov (United States)

    Buyukdagli, Sahin; Ala-Nissila, T

    2013-07-28

    We investigate the modification of gas phase ion polarizabilities upon solvation in polar solvents and ionic liquids. To this aim, we develop a classical electrostatic theory of charged liquids composed of solvent molecules modeled as finite size dipoles, and embedding polarizable ions that consist of Drude oscillators. In qualitative agreement with ab initio calculations of polar solvents and ionic liquids, the hydration energy of a polarizable ion in both types of dielectric liquid is shown to favor the expansion of its electronic cloud. Namely, the ion carrying no dipole moment in the gas phase acquires a dipole moment in the liquid environment, but its electron cloud also reaches an enhanced rigidity. We find that the overall effect is an increase of the gas phase polarizability upon hydration. In the specific case of ionic liquids, it is shown that this hydration process is driven by a collective solvation mechanism where the dipole moment of a polarizable ion induced by its interaction with surrounding ions self-consistently adds to the polarization of the liquid, thereby amplifying the dielectric permittivity of the medium in a substantial way. We propose this self-consistent hydration as the underlying mechanism behind the high dielectric permittivities of ionic liquids composed of small charges with negligible gas phase dipole moment. Hydration being a correlation effect, the emerging picture indicates that electrostatic correlations cannot be neglected in polarizable liquids.

  18. Feeling but not caring: empathic alteration in narcissistic men with high psychopathic traits.

    Science.gov (United States)

    Marcoux, Louis-Alexandre; Michon, Pierre-Emmanuel; Lemelin, Sophie; Voisin, Julien A; Vachon-Presseau, Etienne; Jackson, Philip L

    2014-12-30

    Psychopathy is a personality disorder characterized by specific interpersonal-affective deficits and social deviance often marked by reduced empathy and decreased affective response to the suffering of others. However, recent findings in community samples suggest that the somatosensory resonance to other's pain measured with electroencephalography (EEG) is increased by psychopathic traits. This study aimed at comparing both the response to physical pain and the observation of pain being inflicted to another person in individuals with clinically significant psychopathic traits, namely patients with severe narcissistic personality disorder (NPD, n=11), and community controls (CC, n=13). The gating of somatosensory responses to a tactile steady-state stimulation (25 Hz) during the observation of pain-evoking and non-painful visual stimuli of hands was measured using EEG. Pain thresholds were assessed with a quantitative sensory testing (QST) battery. NPD compared with CC subjects showed similar thermal pain thresholds, but significantly higher pain pressure thresholds (PPT). Significantly greater somatosensory gating (SG) during the anticipation and the observation of pain in others was observed in NPD compared with CC subjects, but this difference was not associated with differences in self-pain perception. SG to pain observation was positively correlated with the Impulsivity-Egocentricity (IE) dimension of psychopathy. These findings demonstrated a stronger somatosensory resonance in the high psychopathic trait NPD group that suggests an increased somatic representation of observed pain despite lower dispositional empathy.

  19. The nitrogen cycle in highly urbanized tropical regions and the effect of river-aquifer interactions: The case of Jakarta and the Ciliwung River

    Science.gov (United States)

    Costa, Diogo; Burlando, Paolo; Priadi, Cindy; Shie-Yui, Liong

    2016-09-01

    Groundwater is extensively used in Jakarta to compensate for the limited public water supply network. Recent observations show a rise in nitrate (NO3-) levels in the shallow aquifer, thus pointing at a potential risk for public health. The detected levels are still below national and international regulatory limits for drinking water but a strategy is necessary to contain the growing problem. We combine 3 years of available data in the Ciliwung River, the major river flowing through Jakarta, with a distributed river-aquifer interaction model to characterise the impact of urbanisation on the N-cycle of both surface and groundwater systems. Results show that the N-cycle in the river-aquifer system is heterogeneous in space, seasonal dependent (i.e. flow regime) and strongly affected by urban pollution. Results suggest also that although the main sources of N related groundwater pollution are leaking septic tanks, the aquifer interaction with the Ciliwung River may locally have a strong effect on the concentrations. In the general context of pollution control in urban areas, this study demonstrates how advanced process-based models can be efficiently used in combination with field measurements to bring new insights into complex contamination problems. These are essential for more effective and integrated management of water quality in river-aquifer systems.

  20. Temporal and spatial distribution of alteration, mineralization and fluid inclusions in the transitional high-sulfidation epithermal-porphyry copper system at Red Mountain, Arizona

    Science.gov (United States)

    Lecumberri-Sanchez, Pilar; Newton, M. Claiborne; Westman, Erik C.; Kamilli, Robert J.; Canby, Vertrees M.; Bodnar, Robert J.

    2013-01-01

    Red Mountain, Arizona, is a Laramide porphyry Cu system (PCD) that has experienced only a modest level of erosion compared to most other similar deposits in the southwestern United States. As a result, the upper portion of the magmatic–hydrothermal system, which represents the transition from shallower high-sulfidation epithermal mineralization to deeper porphyry Cu mineralization, is well preserved. Within the Red Mountain system, alteration, mineralization and fluid inclusion assemblages show a systematic distribution in both time and space. Early-potassic alteration (characterized by the minerals biotite and magnetite) is paragenetically earlier than late-potassic alteration (K-feldspar–anhydrite) and both are followed by later phyllic (sericite–pyrite) alteration. Advanced argillic alteration (pyrophyllite–alunite–other clay minerals) is thought to be coeval with or postdate phyllic alteration. Minerals characteristic of advanced argillic alteration are present in the near surface. Phyllic alteration extends to greater depths compared to advanced argillic alteration. Early-potassic and late-potassic alteration are only observed in the deepest part of the system. Considerable overlap of phyllic alteration with both early-potassic and late-potassic alteration zones is observed. The hypogene mineralization contains 0.4–1.2% Cu and is spatially and temporally related to the late-potassic alteration event. Molybdenum concentration is typically In the deepest part of the system, an early generation of low-to-moderate density and salinity liquid + vapor inclusions with opaque daughter minerals is followed in time by halite-bearing inclusions that also contain opaque daughter minerals indicating that an early intermediate-density magmatic fluid evolved to a high-density, high-salinity mineralizing fluid. The increase in density and salinity of fluids with time observed in the deeper parts of the system may be the result of immiscibility (“boiling”) of

  1. Shortening Infusion Time for High-Dose Methotrexate Alters Antileukemic Effects: A Randomized Prospective Clinical Trial

    Science.gov (United States)

    Mikkelsen, Torben S.; Sparreboom, Alex; Cheng, Cheng; Zhou, Yinmei; Boyett, James M.; Raimondi, Susana C.; Panetta, John C.; Bowman, W. Paul; Sandlund, John T.; Pui, Ching-Hon; Relling, Mary V.; Evans, William E.

    2011-01-01

    Purpose To determine whether shortening the infusion duration of high-dose methotrexate (HDMTX; 1 g/m2) affects the in vivo accumulation of active methotrexate polyglutamates (MTXPG1-7) in leukemia cells and whether this differs among major acute lymphoblastic leukemia (ALL) subtypes. Methods From June 2000 through October 2007, 356 children with ALL were randomly assigned to receive initial single-agent treatment with HDMTX (1 g/m2) as either a 24-hour infusion or a 4-hour infusion at two pediatric hospitals in the United States. The primary outcome measures were the accumulation of MTXPG1-7 in leukemia cells and the antileukemic effects (eg, inhibition of de novo purine synthesis in bone marrow ALL cells, and decrease in circulating ALL cells). Results The 24-hour infusion resulted in significantly higher amounts of MTXPG1-7 in bone marrow leukemia cells (median: 1,695 v 1,150 pmol/109 cells, P = .0059), and better antileukemic effects. The 24-hour infusion had the greatest effect on MTXPG1-7 accumulation in hyperdiploid ALL (median: 3,919 v 2,417 pmol/109 cells, P = .0038); T-cell ALL exhibited smaller differences in MTXPG1-7 but greater antileukemic effects with the longer infusion (median decrease in leukemia cells: 88.4% v 51.8%, P = .0075). In contrast, infusion duration had no significant impact on MTXPG1-7 accumulation or antileukemic effects in ALL with the t(12;21)/(ETV6-RUNX1) chromosomal translocation. Conclusion Shortening the infusion time of HDMTX reduces accumulation of active methotrexate in leukemia cells and decreases antileukemic effects, with differing consequences among major ALL subtypes. PMID:21444869

  2. Neurodegeneration Alters Metabolic Profile and Sirt 1 Signaling in High-Fat-Induced Obese Mice.

    Science.gov (United States)

    Lima, Leandro Ceotto Freitas; Saliba, Soraya Wilke; Andrade, João Marcus Oliveira; Cunha, Maria Luisa; Cassini-Vieira, Puebla; Feltenberger, John David; Barcelos, Lucíola Silva; Guimarães, André Luiz Sena; de-Paula, Alfredo Mauricio Batista; de Oliveira, Antônio Carlos Pinheiro; Santos, Sérgio Henrique Sousa

    2016-05-16

    Different factors may contribute to the development of neurodegenerative diseases. Among them, metabolic syndrome (MS), which has reached epidemic proportions, has emerged as a potential element that may be involved in neurodegeneration. Furthermore, studies have shown the importance of the sirtuin family in neuronal survival and MS, which opens the possibility of new pharmacological targets. This study investigates the influence of sirtuin metabolic pathways by examining the functional capacities of glucose-induced obesity in an excitotoxic state induced by a quinolinic acid (QA) animal model. Mice were divided into two groups that received different diets for 8 weeks: one group received a regular diet, and the other group received a high-fat diet (HF) to induce MS. The animals were submitted to a stereotaxic surgery and subdivided into four groups: Standard (ST), Standard-QA (ST-QA), HF and HF-QA. The QA groups were given a 250 nL quinolinic acid injection in the right striatum and PBS was injected in the other groups. Obese mice presented with a weight gain of 40 % more than the ST group beyond acquiring an insulin resistance. QA induced motor impairment and neurodegeneration in both ST-QA and HF-QA, although no difference was observed between these groups. The HF-QA group showed a reduction in adiposity when compared with the groups that received PBS. Therefore, the HF-QA group demonstrated a commitment-dependent metabolic pathway. The results suggest that an obesogenic diet does not aggravate the neurodegeneration induced by QA. However, the excitotoxicity induced by QA promotes a sirtuin pathway impairment that contributes to metabolic changes.

  3. High resolution observations of the near-surface wind field over an isolated mountain and in a steep river canyon

    Directory of Open Access Journals (Sweden)

    B. W. Butler

    2014-06-01

    Full Text Available A number of numerical wind flow models have been developed for simulating wind flow at relatively fine spatial resolutions (e.g., ∼100 m; however, there are very limited observational data available for evaluating these high resolution models. This study presents high-resolution surface wind datasets collected from an isolated mountain and a steep river canyon. The wind data are presented in terms of four flow regimes: upslope, afternoon, downslope, and a synoptically-driven regime. There were notable differences in the data collected from the two terrain types. For example, wind speeds collected on the isolated mountain increased with distance upslope during upslope flow, but generally decreased with distance upslope at the river canyon site during upslope flow. Wind speed did not have a simple, consistent trend with position on the slope during the downslope regime on the isolated mountain, but generally increased with distance upslope at the river canyon site. The highest measured speeds occurred during the passage of frontal systems on the isolated mountain. Mountaintop winds were often twice as high as wind speeds measured on the surrounding plain. The highest speeds measured in the river canyon occurred during late morning hours and were from easterly downcanyon flows, presumably associated with surface pressure gradients induced by formation of a regional thermal trough to the west and high pressure to the east. Under periods of weak synoptic forcing, surface winds tended to be decoupled from large-scale flows, and under periods of strong synoptic forcing, variability in surface winds was sufficiently large due to terrain-induced mechanical effects (speed-up over ridges and decreased speeds on leeward sides of terrain obstacles that a large-scale mean flow would not be representative of surface winds at most locations on or within the terrain feature. These findings suggest that traditional operational weather model (i.e., with

  4. Formulation of SYNROC-D additives for Savannah River Plant high-level radioactive waste. [ADSYN code

    Energy Technology Data Exchange (ETDEWEB)

    Ryerson, F.J.; Burr, K.; Rozsa, R.

    1981-12-01

    SYNROC-D is a multiphase ceramic waste form consisting of nepheline, zirconolite, perovskite, and spinel. It has been formulated for the immobilization of high-level radioactive wastes now stored at Savannah River Plant (SRP) near Aiken, South Carolina. This report utilizes existing experimental data to develop a method for calculating additives to these waste products. This method calculates additions based on variations of mineral compositions as a function of sludge composition and radionuclide partitioning among the SYNROC phases. Based on these calculations, a FORTRAN program called ADSYN has been developed to determine the proper reagent proportions to be added to the SRP sludges.

  5. Effective use of principal component analysis with high resolution remote sensing data to delineate hydrothermal alteration and carbonate rocks

    Science.gov (United States)

    Feldman, Sandra C.

    1987-01-01

    Methods of applying principal component (PC) analysis to high resolution remote sensing imagery were examined. Using Airborne Imaging Spectrometer (AIS) data, PC analysis was found to be useful for removing the effects of albedo and noise and for isolating the significant information on argillic alteration, zeolite, and carbonate minerals. An effective technique for using PC analysis using an input the first 16 AIS bands, 7 intermediate bands, and the last 16 AIS bands from the 32 flat field corrected bands between 2048 and 2337 nm. Most of the significant mineralogical information resided in the second PC. PC color composites and density sliced images provided a good mineralogical separation when applied to a AIS data set. Although computer intensive, the advantage of PC analysis is that it employs algorithms which already exist on most image processing systems.

  6. Highly active modulators of indole signaling alter pathogenic behaviors in Gram-negative and Gram-positive bacteria.

    Science.gov (United States)

    Minvielle, Marine J; Eguren, Kristen; Melander, Christian

    2013-12-16

    Indole is a universal signal that regulates various bacterial behaviors, such as biofilm formation and antibiotic resistance. To generate mechanistic probes of indole signaling and control indole-mediated pathogenic phenotypes in both Gram-positive and Gram-negative bacteria, we have investigated the use of desformylflustrabromine (dFBr) derivatives to generate highly active indole mimetics. We have developed non-microbicidal dFBr derivatives that are 27-2000 times more active than indole in modulating biofilm formation, motility, acid resistance, and antibiotic resistance. The activity of these analogues parallels indole, because they are dependent on temperature, the enzyme tryptophanase TnaA, and the transcriptional regulator SdiA. This investigation demonstrates that molecules based on the dFBr scaffold can alter pathogenic behaviors by mimicking indole-signaling pathways.

  7. Cyclophosphamide alters the gene expression profile in patients treated with high doses prior to stem cell transplantation.

    Directory of Open Access Journals (Sweden)

    Ibrahim El-Serafi

    Full Text Available BACKGROUND: Hematopoietic stem cell transplantation is a curative treatment for several haematological malignancies. However, treatment related morbidity and mortality still is a limiting factor. Cyclophosphamide is widely used in condition regimens either in combination with other chemotherapy or with total body irradiation. METHODS: We present the gene expression profile during cyclophosphamide treatment in 11 patients conditioned with cyclophosphamide for 2 days followed by total body irradiation prior to hematopoietic stem cell transplantation. 299 genes were identified as specific for cyclophosphamide treatment and were arranged into 4 clusters highly down-regulated genes, highly up-regulated genes, early up-regulated but later normalized genes and moderately up-regulated genes. RESULTS: Cyclophosphamide treatment down-regulated expression of several genes mapped to immune/autoimmune activation and graft rejection including CD3, CD28, CTLA4, MHC II, PRF1, GZMB and IL-2R, and up-regulated immune-related receptor genes, e.g. IL1R2, IL18R1, and FLT3. Moreover, a high and significant expression of ANGPTL1 and c-JUN genes was observed independent of cyclophosphamide treatment. CONCLUSION: This is the first investigation to provide significant information about alterations in gene expression following cyclophosphamide treatment that may increase our understanding of the cyclophosphamide mechanism of action and hence, in part, avoid its toxicity. Furthermore, ANGPTL1 remained highly expressed throughout the treatment and, in contrast to several other alkylating agents, cyclophosphamide did not influence c-JUN expression.

  8. Alteration of MX-80 by hydrothermal treatment under high salt content conditions

    Energy Technology Data Exchange (ETDEWEB)

    Pusch, R. [Geodevelopment AB, Lund (Sweden); Kasbohm, J. [Greifswald Univ. (Germany). Geological Dep.

    2002-02-01

    If brammalit, i.e. sodium illite, is formed from smectite in Na-rich salt water at high temperature such conversion can also take place in the buffer clay that surrounds the canisters in a KBS-3 repository. The present study comprised two laboratory test series with MX-80 clay, one with compacted clay powder with a dry density of 1200 to 1300 kg/m{sup 3} and saturation with 10% and 20% NaCl solutions followed by heating to 110 deg C under closed conditions for 30 days. In the second series air-dry compacted clay powder in a cell was heated at 110 deg C for the same period of time and connected to vessels with 10% and 20% NaCl solutions. The first series represents the conditions in the buffer clay after saturation with Na-rich salt water while the second one corresponds to the conditions in the course of saturation with such water. All laboratory tests were made after short-term percolation with distilled water for making sure that the hydro-thermally treated samples were fully fluid-saturated. The results from the physical testing showed that the hydraulic conductivity and swelling pressure of the hydrothermally treated clay samples were on the same order of magnitude as for untreated clay. Comparison with illitic clays shows that the latter are at least a hundred times more permeable than the hydrothermally treated salt clays in the present study, which hence indicates that conversion to illite was insignificant. This is obvious also from the fact that while illitic clays have very low swelling pressures the hydrothermally treated clays exhibited swelling pressures on the same order of magnitude as untreated MX-80. XRD analysis showed a clear difference in mineral constitution between the two test series. Thus, while no significant change from the typical mineralogy of untreated MX-80 was found for hydrothermal treatment of clay saturated with 10 and 20% NaCl solution, except for some very slight neoformation of illite-smectite mixed layers or irreversible

  9. Alteration of MX-80 by hydrothermal treatment under high salt content conditions

    Energy Technology Data Exchange (ETDEWEB)

    Pusch, R. [Geodevelopment AB, Lund (Sweden); Kasbohm, J. [Greifswald Univ. (Germany). Geological Dep.

    2002-02-01

    If brammalit, i.e. sodium illite, is formed from smectite in Na-rich salt water at high temperature such conversion can also take place in the buffer clay that surrounds the canisters in a KBS-3 repository. The present study comprised two laboratory test series with MX-80 clay, one with compacted clay powder with a dry density of 1200 to 1300 kg/m{sup 3} and saturation with 10% and 20% NaCl solutions followed by heating to 110 deg C under closed conditions for 30 days. In the second series air-dry compacted clay powder in a cell was heated at 110 deg C for the same period of time and connected to vessels with 10% and 20% NaCl solutions. The first series represents the conditions in the buffer clay after saturation with Na-rich salt water while the second one corresponds to the conditions in the course of saturation with such water. All laboratory tests were made after short-term percolation with distilled water for making sure that the hydro-thermally treated samples were fully fluid-saturated. The results from the physical testing showed that the hydraulic conductivity and swelling pressure of the hydrothermally treated clay samples were on the same order of magnitude as for untreated clay. Comparison with illitic clays shows that the latter are at least a hundred times more permeable than the hydrothermally treated salt clays in the present study, which hence indicates that conversion to illite was insignificant. This is obvious also from the fact that while illitic clays have very low swelling pressures the hydrothermally treated clays exhibited swelling pressures on the same order of magnitude as untreated MX-80. XRD analysis showed a clear difference in mineral constitution between the two test series. Thus, while no significant change from the typical mineralogy of untreated MX-80 was found for hydrothermal treatment of clay saturated with 10 and 20% NaCl solution, except for some very slight neoformation of illite-smectite mixed layers or irreversible

  10. cDNA microarray reveals the alterations of cytoskeleton-related genes in osteoblast under high magneto-gravitational environment

    Institute of Scientific and Technical Information of China (English)

    Airong Qian; Shengmeng Di; Xiang Gao; Wei Zhang; Zongcheng Tian; Jingbao Li; Lifang Hu; Pengfei Yang; Dachuan Yin; Peng Shang

    2009-01-01

    The diamagnetic levitation as a novel ground-based model for simulating a reduced gravity environment has been widely applied in many fields.In this study,a special designed superconducting magnet,which can produce three apparent gravity levels (0,1,and 2 g),namely high magneto-gravitational environment (HMGE),was used to simulate space gravity environment.The effects of HMGE on osteoblast gene expression profile were investigated by microarray.Genes sensitive to diamagnetic levitation environment (0 g),gravity changes,and high magnetic field changes were sorted on the basis of typical cell func-tions.Cytoskeleton,as an intracellular load-bearing struc-ture,plays an important role in gravity perception.Therefore,13 cytoskeleton-related genes were chosen according to the results of microarray analysis,and the expressions of these genes were found to be altered under HMGE by real-time PCR.Based on the PCR results,the expressions of WASF2 (WAS protein family,member 2),WIPFI (WAS/WASL interacting protein family,member 1),paxillin:and talin 1 were further identified by western blot assay.Results indicated that WASF2 and WIPF1 were more sensitive to altered gravity levels,and talin 1 and paxillin were sensitive to both magnetic field and gravity changes.Our findings demonstrated that HMGE can affect osteoblast gene expression profile and cytoskele-ton-related genes expression.The identification of mechanosensitive genes may enhance our understandings to the mechanism of bone loss induced by microgravity and may provide some potential targets for preventing and treating bone loss or osteoporosis.

  11. Using high-throughput sequencing to assess the impacts of treated and untreated wastewater discharge on prokaryotic communities in an urban river.

    Science.gov (United States)

    Bai, Yaohui; Qi, Weixiao; Liang, Jinsong; Qu, Jiuhui

    2014-02-01

    In many megacities wastewater is an important source of surface water, particularly during drought periods. While changes in surface water chemistry associated with effluent inflow have generally been well-studied, few data have been collected on the effects to prokaryotic communities. The objective of this study was to explore the impacts of treated and untreated wastewater discharges on prokaryotic community in an urban river. High-throughput sequencing was conducted for analyzing the prokaryotic community composition and function in river water, treated wastewater and untreated wastewater. Results revealed that the prokaryotic community compositions in the upstream river reach were dominated by treated wastewater discharge. In the middle- and downstream river reaches, untreated effluent volumes are higher, thus affecting the structure of the prokaryotic community, promoting a rise in Cyanobacteria and Thaumarchaeota. Function annotation revealed a number of genes associated with xenobiotic metabolism and human diseases were observed in river and wastewater samples, suggesting wastewater discharge to river may pose a risk to human health. Quantitative real-time PCR results revealed that the treated and untreated wastewater discharges also affected the abundance of ammonia oxidation bacteria (AOB) and ammonia oxidation archaea (AOA) in river.

  12. Effect of irrigation pumpage during drought on karst aquifer systems in highly agricultural watersheds: example of the Apalachicola-Chattahoochee-Flint river basin, southeastern USA

    Science.gov (United States)

    Mitra, Subhasis; Srivastava, Puneet; Singh, Sarmistha

    2016-09-01

    In the Apalachicola-Chattahoochee-Flint (ACF) river basin in Alabama, Georgia, and Florida (USA), population growth in the city of Atlanta and increased groundwater withdrawal for irrigation in southwest Georgia are greatly affecting the supply of freshwater to downstream regions. This study was conducted to understand and quantify the effect of irrigation pumpage on the karst Upper Floridan Aquifer and river-aquifer interactions in the lower ACF river basin in southwest Georgia. The groundwater MODular Finite-Element model (MODFE) was used for this study. The effect of two drought years, a moderate and a severe drought year, were simulated. Comparison of the results of the irrigated and non-irrigated scenarios showed that groundwater discharge to streams is a major outflow from the aquifer, and irrigation can cause as much as 10 % change in river-aquifer flux. The results also show that during months with high irrigation (e.g., June 2011), storage loss (34 %), the recharge and discharge from the upper semi-confining unit (30 %), and the river-aquifer flux (31 %) are the major water components contributing towards the impact of irrigation pumpage in the study area. A similar scenario plays out in many river basins throughout the world, especially in basins in which underlying karst aquifers are directly connected to a nearby stream. The study suggests that improved groundwater withdrawal strategies using climate forecasts needs to be developed in such a way that excessive withdrawals during droughts can be reduced to protect streams and river flows.

  13. A Natural-Rule-Based-Connection (NRBC Method for River Network Extraction from High-Resolution Imagery

    Directory of Open Access Journals (Sweden)

    Chuiqing Zeng

    2015-10-01

    Full Text Available This study proposed a natural-rule-based-connection (NRBC method to connect river segments after water body detection from remotely sensed imagery. A complete river network is important for many hydrological applications. While water body detection methods using remote sensing are well-developed, less attention has been paid to connect discontinuous river segments and form a complete river network. This study designed an automated NRBC method to extract a complete river network by connecting river segments at polygon level. With the assistance of an image pyramid, neighbouring river segments are connected based on four criteria: gap width (Tg, river direction consistency (Tθ, river width consistency (Tw, and minimum river segment length (Tl. The sensitivity of these four criteria were tested, analyzed, and proper criteria values were suggested using image scenes from two diverse river cases. The comparison of NRBC and the alternative morphological method demonstrated NRBC’s advantage of natural rule based selective connection. We refined a river centerline extraction method and show how it outperformed three other existing centerline extraction methods on the test sites. The extracted river polygons and centerlines have a multitude of end uses including rapidly mapping flood extents, monitoring surface water supply, and the provision of validation data for simulation models required for water quantity, quality and aquatic biota assessments. The code for the NRBC is available on GitHub.

  14. Amazon river dolphins (Inia geoffrensis) use a high-frequency short-range biosonar

    DEFF Research Database (Denmark)

    Ladegaard, Michael; Jensen, Frants Havmand; de Freitas, Mafalda

    2015-01-01

    Toothed whales produce echolocation clicks with source parameters related to body size; however, it may be equally important to consider the influence of habitat, as suggested by studies on echolocating bats. A few toothed whale species have fully adapted to river systems, where sonar operation...... is likely to result in higher clutter and reverberation levels than those experienced by most toothed whales at sea because of the shallow water and dense vegetation. To test the hypothesis that habitat shapes the evolution of toothed whale biosonar parameters by promoting simpler auditory scenes...

  15. [Changes of wetland landscape pattern in Dayang River Estuary based on high-resolution remote sensing image].

    Science.gov (United States)

    Wu, Tao; Zhao, Dong-zhi; Zhang, Feng-shou; Wei, Bao-quan

    2011-07-01

    Based on the comprehensive consideration of the high resolution characteristics of remote sensing data and the current situation of land cover and land use in Dayang River Estuary wetland, a classification system with different resolutions of wetland landscape in the Estuary was established. The landscape pattern indices and landscape transition matrix were calculated by using the high resolution remote sensing data, and the dynamic changes of the landscape pattern from 1984 to 2008 were analyzed. In the study period, the wetland landscape components changed drastically. Wetland landscape transferred from natural wetland into artificial wetland, and wetland core regional area decreased. Natural wetland's largest patch area index descended, and the fragmentation degree ascended; while artificial wetland area expanded, its patch number decreased, polymerization degree increased, and the maximum patch area index had an obvious increasing trend. Increasing human activities, embankment construction, and reclamation for aquaculture were the main causes for the decrease of wetland area and the degradation of the ecological functions of Dayang River Estuary. To constitute long-term scientific and reasonable development plan, establish wetland nature reserves, protect riverway, draft strict inspective regimes for aquaculture reclamation, and energetically develop resource-based tourism industry would be the main strategies for the protection of the estuarine wetland.

  16. High genetic diversity in cryptic populations of the migratory sutchi catfish Pangasianodon hypophthalmus in the Mekong River.

    Science.gov (United States)

    So, N; Maes, G E; Volckaert, F A M

    2006-02-01

    The detection and conservation of spawning units is of crucial importance in highly migratory species. The sutchi catfish Pangasianodon hypophthalmus (Pangasiidae; Teleostei) is a common large-sized tropical fish, which migrates annually to several upstream spawning sites on the Lower Mekong River and feeds on the huge floodplain of the Lower Mekong and Tonle Sap for the other half of the year. We hypothesised that because of the relative size of the feeding and spawning habitat, genetic variability would be high and homogeneous in foraging populations, but that spawning stocks would be distinct in space and time. To test these predictions, 567 individuals from 10 geographic locations separated by up to 1230 km along the Lower Mekong River were genotyped at seven microsatellite loci. The level of genetic diversity was much higher than other freshwater fish and reached values comparable to marine species (mean H(e)=0.757). All samples collected at the potential spawning sites deviated from Hardy-Weinberg expectations, suggesting admixture. Individual-based clustering methods revealed genetic heterogeneity and enabled the detection of three genetically distinct sympatric populations. There was no evidence of recent reduction in effective population size in any population. Contrasting with the vast extent of the feeding grounds, the shortage of spawning grounds seems to have moved sutchi catfish towards diachronous spawning. Hence the sustainable exploitation of this natural resource hinges on the conservation of the limited spawning grounds and open migration routes between the spawning and feeding grounds.

  17. Landscape genetics informs mesohabitat preference and conservation priorities for a surrogate indicator species in a highly fragmented river system.

    Science.gov (United States)

    Lean, J; Hammer, M P; Unmack, P J; Adams, M; Beheregaray, L B

    2017-04-01

    Poor dispersal species represent conservative benchmarks for biodiversity management because they provide insights into ecological processes influenced by habitat fragmentation that are less evident in more dispersive organisms. Here we used the poorly dispersive and threatened river blackfish (Gadopsis marmoratus) as a surrogate indicator system for assessing the effects of fragmentation in highly modified river basins and for prioritizing basin-wide management strategies. We combined individual, population and landscape-based approaches to analyze genetic variation in samples spanning the distribution of the species in Australia's Murray-Darling Basin, one of the world's most degraded freshwater systems. Our results indicate that G. marmoratus displays the hallmark of severe habitat fragmentation with notably scattered, small and demographically isolated populations with very low genetic diversity-a pattern found not only between regions and catchments but also between streams within catchments. By using hierarchically nested population sampling and assessing relationships between genetic uniqueness and genetic diversity across populations, we developed a spatial management framework that includes the selection of populations in need of genetic rescue. Landscape genetics provided an environmental criterion to identify associations between landscape features and ecological processes. Our results further our understanding of the impact that habitat quality and quantity has on habitat specialists with similarly low dispersal. They should also have practical applications for prioritizing both large- and small-scale conservation management actions for organisms inhabiting highly fragmented ecosystems.

  18. Effects of acid mine drainage on fish and macroinvertebrates of the Tioga River, Pennsylvania and New York

    Science.gov (United States)

    Barker, James L.

    1972-01-01

    Acid water from abandoned coal mines in the vicinity of Morris Run and Blossburg, Pa., severely alter the aquatic environment of the Tioga River. From Morris Run to Crooked Creek, a reach of 19 miles, the river bed is influenced by a smothering blanket of heavy metal precipitates and highly acidic water. Biologically, this reach of the river is devoid of fishlife and nearly devoid of benthic macroinvertebrates. Downstream from Crooked Creek the water quality and biota are slowly restored. At Presho, N.Y., the river again supports an abundant and diversified population of fish and bottom-dwelling organisms.

  19. Altered expression of MGMT in high-grade gliomas results from the combined effect of epigenetic and genetic aberrations.

    Directory of Open Access Journals (Sweden)

    João Ramalho-Carvalho

    Full Text Available MGMT downregulation in high-grade gliomas (HGG has been mostly attributed to aberrant promoter methylation and is associated with increased sensitivity to alkylating agent-based chemotherapy. However, HGG harboring 10q deletions also benefit from treatment with alkylating agents. Because the MGMT gene is mapped at 10q26, we hypothesized that both epigenetic and genetic alterations might affect its expression and predict response to chemotherapy. To test this hypothesis, promoter methylation and mRNA levels of MGMT were determined by quantitative methylation-specific PCR (qMSP or methylation-specific multiplex ligation dependent probe amplification (MS-MLPA and quantitative RT-PCR, respectively, in a retrospective series of 61 HGG. MGMT/chromosome 10 copy number variations were determined by FISH or MS-MLPA analysis. Molecular findings were correlated with clinical parameters to assess their predictive value. Overall, MGMT methylation ratios assessed by qMSP and MS-MLPA were inversely correlated with mRNA expression levels (best coefficient value obtained with MS-MLPA. By FISH analysis in 68.3% of the cases there was loss of 10q26.1 and in 15% of the cases polysomy was demonstrated; the latter displayed the highest levels of transcript. When genetic and epigenetic data were combined, cases with MGMT promoter methylation and MGMT loss depicted the lowest transcript levels, although an impact in response to alkylating agent chemotherapy was not apparent. Cooperation between epigenetic (promoter methylation and genetic (monosomy, locus deletion changes affecting MGMT in HGG is required for effective MGMT silencing. Hence, evaluation of copy number alterations might add relevant prognostic and predictive information concerning response to alkylating agent-based chemotherapy.

  20. Convergent Findings of Altered Functional and Structural Brain Connectivity in Individuals with High Functioning Autism: A Multimodal MRI Study.

    Science.gov (United States)

    Mueller, Sophia; Keeser, Daniel; Samson, Andrea C; Kirsch, Valerie; Blautzik, Janusch; Grothe, Michel; Erat, Okan; Hegenloh, Michael; Coates, Ute; Reiser, Maximilian F; Hennig-Fast, Kristina; Meindl, Thomas

    2013-01-01

    Brain tissue changes in autism spectrum disorders seem to be rather subtle and widespread than anatomically distinct. Therefore a multimodal, whole brain imaging technique appears to be an appropriate approach to investigate whether alterations in white and gray matter integrity relate to consistent changes in functional resting state connectivity in individuals with high functioning autism (HFA). We applied diffusion tensor imaging (DTI), voxel-based morphometry (VBM) and resting state functional connectivity magnetic resonance imaging (fcMRI) to assess differences in brain structure and function between 12 individuals with HFA (mean age 35.5, SD 11.4, 9 male) and 12 healthy controls (mean age 33.3, SD 9.0, 8 male). Psychological measures of empathy and emotionality were obtained and correlated with the most significant DTI, VBM and fcMRI findings. We found three regions of convergent structural and functional differences between HFA participants and controls. The right temporo-parietal junction area and the left frontal lobe showed decreased fractional anisotropy (FA) values along with decreased functional connectivity and a trend towards decreased gray matter volume. The bilateral superior temporal gyrus displayed significantly decreased functional connectivity that was accompanied by the strongest trend of gray matter volume decrease in the temporal lobe of HFA individuals. FA decrease in the right temporo-parietal region was correlated with psychological measurements of decreased emotionality. In conclusion, our results indicate common sites of structural and functional alterations in higher order association cortex areas and may therefore provide multimodal imaging support to the long-standing hypothesis of autism as a disorder of impaired higher-order multisensory integration.

  1. Convergent Findings of Altered Functional and Structural Brain Connectivity in Individuals with High Functioning Autism: A Multimodal MRI Study.

    Directory of Open Access Journals (Sweden)

    Sophia Mueller

    Full Text Available Brain tissue changes in autism spectrum disorders seem to be rather subtle and widespread than anatomically distinct. Therefore a multimodal, whole brain imaging technique appears to be an appropriate approach to investigate whether alterations in white and gray matter integrity relate to consistent changes in functional resting state connectivity in individuals with high functioning autism (HFA. We applied diffusion tensor imaging (DTI, voxel-based morphometry (VBM and resting state functional connectivity magnetic resonance imaging (fcMRI to assess differences in brain structure and function between 12 individuals with HFA (mean age 35.5, SD 11.4, 9 male and 12 healthy controls (mean age 33.3, SD 9.0, 8 male. Psychological measures of empathy and emotionality were obtained and correlated with the most significant DTI, VBM and fcMRI findings. We found three regions of convergent structural and functional differences between HFA participants and controls. The right temporo-parietal junction area and the left frontal lobe showed decreased fractional anisotropy (FA values along with decreased functional connectivity and a trend towards decreased gray matter volume. The bilateral superior temporal gyrus displayed significantly decreased functional connectivity that was accompanied by the strongest trend of gray matter volume decrease in the temporal lobe of HFA individuals. FA decrease in the right temporo-parietal region was correlated with psychological measurements of decreased emotionality. In conclusion, our results indicate common sites of structural and functional alterations in higher order association cortex areas and may therefore provide multimodal imaging support to the long-standing hypothesis of autism as a disorder of impaired higher-order multisensory integration.

  2. Lingonberries alter the gut microbiota and prevent low-grade inflammation in high-fat diet fed mice

    Directory of Open Access Journals (Sweden)

    Lovisa Heyman-Lindén

    2016-04-01

    Full Text Available Background: The gut microbiota plays an important role in the development of obesity and obesity-associated impairments such as low-grade inflammation. Lingonberries have been shown to prevent diet-induced obesity and low-grade inflammation. However, it is not known whether the effect of lingonberry supplementation is related to modifications of the gut microbiota. The aim of the present study was to describe whether consumption of different batches of lingonberries alters the composition of the gut microbiota, which could be relevant for the protective effect against high fat (HF-induced metabolic alterations. Methods: Three groups of C57BL/6J mice were fed HF diet with or without a supplement of 20% lingonberries from two different batches (Lingon1 and Lingon2 during 11 weeks. The composition and functionality of the cecal microbiota were assessed by 16S rRNA sequencing and PICRUSt. In addition, parameters related to obesity, insulin sensitivity, hepatic steatosis, inflammation and gut barrier function were examined. Results: HF-induced obesity was only prevented by the Lingon1 diet, whereas both batches of lingonberries reduced plasma levels of markers of inflammation and endotoxemia (SAA and LBP as well as modified the composition and functionality of the gut microbiota, compared to the HF control group. The relative abundance of Akkermansia and Faecalibacterium, genera associated with healthy gut mucosa and anti-inflammation, was found to increase in response to lingonberry intake. Conclusions: Our results show that supplementation with lingonberries to an HF diet prevents low-grade inflammation and is associated with significant changes of the microbiota composition. Notably, the anti-inflammatory properties of lingonberries seem to be independent of effects on body weight gain.

  3. Altered topological organization of high-level visual networks in Alzheimer's disease and mild cognitive impairment patients.

    Science.gov (United States)

    Deng, Yanjia; Shi, Lin; Lei, Yi; Wang, Defeng

    2016-09-06

    Altered regional activation of high-level visual (HLV) cortices in patients with Alzheimer's disease (AD) or mild cognitive impairment (MCI) has been well documented in previous fMRI studies, which led us to investigate the underlying alteration of the HLV networks in the terms of intrinsic interaction and topological organization. First, the activation likelihood estimation, a coordinate-based meta-analysis approach, was used to define the cortical regions/nodes included in HLV networks of "what" and "where" visions. Secondly, the acquired HLV regions were used as seeds to calculate their interregional resting-state functional connectivities (RSFCs) based on the temporal correlation of rs-functional MRI (rs-fMRI) time series. Here, the rs-fMRI data of AD (n=30), late MCI (n=35), early MCI (n=52) and matched healthy controls (n=44) were obtained from the Alzheimer's Disease Neuroimaging Initiative dataset. Finally, based on the calculated pair-wise RSFCs, the "what" and "where" HLV networks were respectively constructed, and their topological properties were calculated and analyzed among groups using the graph theory method. The results demonstrated increased clustering coefficient combined with a prolonged characteristic path length of the "where" visual network in AD patients. No significant alternation of the "what" visual network was found among the groups. These results suggest that the abnormality of the HLV networks could be a late-stage outcome in AD and that the "where" visual network may be more susceptible to the AD-related neuropathological changes than the "what" visual network. In addition, the dysfunction of the "where" network is found to be characterized by a decreased integration combined with an increased local segregation.

  4. High-Fat Diet-Induced Alterations in the Feeding Suppression of Low-Dose Nisoxetine, a Selective Norepinephrine Reuptake Inhibitor

    OpenAIRE

    Bello, Nicholas T.; Walters, Amy L.; Verpeut, Jessica L.; Priscila P. Cunha

    2013-01-01

    Central noradrenergic pathways are involved in feeding and cardiovascular control, physiological processes altered by obesity. The present studies determined how high-fat feeding and body weight gain alter the sensitivity to the feeding suppression and neural activation to a selective norepinephrine reuptake inhibitor, nisoxetine. Acute administration of nisoxetine (saline: 0, 3, 10, and 30 mg/kg; IP) resulted in a dose-dependent reduction in the 24 h refeeding response in male Sprague Dawley...

  5. RADIOLYTIC HYDROGEN GENERATION INSAVANNAH RIVER SITE (SRS) HIGH LEVEL WASTETANKS COMPARISON OF SRS AND HANFORDMODELING PREDICTIONS

    Energy Technology Data Exchange (ETDEWEB)

    Crawford, C; Ned Bibler, N

    2009-04-15

    In the high level waste tanks at the Savannah River Site (SRS), hydrogen is produced continuously by interaction of the radiation in the tank with water in the waste. Consequently, the vapor spaces of the tanks are purged to prevent the accumulation of H{sub 2} and possible formation of a flammable mixture in a tank. Personnel at SRS have developed an empirical model to predict the rate of H{sub 2} formation in a tank. The basis of this model is the prediction of the G value for H{sub 2} production. This G value is the number of H{sub 2} molecules produced per 100 eV of radiolytic energy absorbed by the waste. Based on experimental studies it was found that the G value for H{sub 2} production from beta radiation and from gamma radiation were essentially equal. The G value for H{sub 2} production from alpha radiation was somewhat higher. Thus, the model has two equations, one for beta/gamma radiation and one for alpha radiation. Experimental studies have also indicated that both G values are decreased by the presence of nitrate and nitrite ions in the waste. These are the main scavengers for the precursors of H{sub 2} in the waste; thus the equations that were developed predict G values for hydrogen production as a function of the concentrations of these two ions in waste. Knowing the beta/gamma and alpha heat loads in the waste allows one to predict the total generation rate for hydrogen in a tank. With this prediction a ventilation rate can be established for each tank to ensure that a flammable mixture is not formed in the vapor space in a tank. Recently personnel at Hanford have developed a slightly different model for predicting hydrogen G values. Their model includes the same precursor for H{sub 2} as the SRS model but also includes an additional precursor not in the SRS model. Including the second precursor for H{sub 2} leads to different empirical equations for predicting the G values for H{sub 2} as a function of the nitrate and nitrite concentrations in

  6. Low and high doses of UV-B differentially modulate chlorpyrifos-induced alterations in nitrogen metabolism of cyanobacteria.

    Science.gov (United States)

    Srivastava, Prabhat Kumar; Singh, Vijay Pratap; Prasad, Sheo Mohan

    2014-09-01

    The present study assessed the comparative responses on the specific growth rate, nitrogen metabolism and enzymes associated with nitrogen metabolism in two nitrogen fixing cyanobacteria-Nostoc muscorum and Phormidium foveolarum exposed to two UV-B doses (low; UV-BL: 0.5472kJm(-2) and high; UV-BH: 5.472kJm(-2)) and two doses of the insecticide chlorpyrifos (O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphorothioate; low i.e. CPL, 1µgml(-1) and high i.e. CPH, 2µgml(-1)) singly and in combination. The specific growth rate, NO3(-) and NO2(-) uptake, nitrate assimilating enzymes - nitrate reductase and nitrite reductase and ammonium assimilating enzymes - glutamine synthetase and glutamate synthase were severely affected when treated either with CPH or/and UV-BH while glutamate dehydrogenase exhibited a stimulatory response. CPL also reduced all the measured parameters (except GDH activity) after 24h, however, a stimulatory effect was observed after 72h due to an increase in nitrogen metabolism (and other antioxidant) enzymes during this period. UV-BL did not cause significant alteration in the studied parameters while in combination with CP doses, it either alleviated the inhibitory effects or further enhanced the CPL induced activities of these enzymes (except GDH). Overall results indicate the resistant nature of P. foveolarum against the inhibitory doses of UV-B and chlorpyrifos in comparison to N. muscorum.

  7. Medial opening wedge high tibial osteotomy alters knee moments in multiple planes during walking and stair ascent.

    Science.gov (United States)

    Leitch, Kristyn M; Birmingham, Trevor B; Dunning, Cynthia E; Giffin, J Robert

    2015-07-01

    Medial opening wedge high tibial osteotomy is a surgical procedure intended to redistribute loads on the knee in patients with medial compartment knee osteoarthritis (OA). The surgery may affect moments in multiple planes during ambulation, with potential beneficial or detrimental effects on joint loads. The objective of this study was to investigate three-dimensional external knee moments before and after medial opening wedge high tibial osteotomy during level walking and during stair ascent. Fourteen patients with varus alignment and osteoarthritis primarily affecting the medial compartment of the tibiofemoral joint were assessed. Three-dimensional motion analyses during level walking and stair ascent was evaluated using inverse dynamics before, 6 and 12 months after surgery. Mean changes at 12 months suggested decreases in the peak knee adduction, flexion and internal rotation moments, with standardized response means ranging from 0.15 to 2.54. These decreases were observed despite increases in speed. Changes in alignment were associated with changes in the adduction and internal rotation moments, but not the flexion moment. Both pre- and postoperatively, the peak knee adduction moment was significantly lower (p=0.001) during stair ascent than during level walking, while the flexion and internal rotation moments were significantly higher (pplanes of motion during ambulation, suggesting substantial alterations of the loads on the knee during ambulation.

  8. Geochemical behavior of radionuclides in highly altered zircon above the Bangombé natural fission reactor, Gabon

    Science.gov (United States)

    Kikuchi, Makiko; Hidaka, Hiroshi; Horie, Kenji

    The isotopic compositions of rare earth elements (REE), Pb and U of highly altered zircons from the clay and black shale layers above the Bangombé natural reactor, Gabon, were determined by a sensitive high resolution ion microprobe (SHRIMP) to discuss the redistribution processes of elements into zircons under the supergene weathering. The clay layer trapped most of the fissiogenic Nd, Sm and Eu derived from the reactor and prevented them migrating into the black shale layer. On the other hand, only the Ce isotopic ratios of the clay and black shale layers have about 2 times larger variations than the other REE. This result suggests that a large chemical fractionation between Ce and other REE above the reactor occurred under the oxidizing condition. The U-Pb data of zircons suggest that the U-Pb system was largely disturbed by migration of chemically fractionated Pb and U from the 2.0 Ga-old uraninite in association with recent weathering.

  9. Altered expression of urokinase-type plasminogen activator and plasminogen activator inhibitor in high-risk soft tissue sarcomas.

    Science.gov (United States)

    Benassi, M S; Ponticelli, F; Azzoni, E; Gamberi, G; Pazzaglia, L; Chiechi, A; Conti, A; Spessotto, P; Scapolan, M; Pignotti, E; Bacchini, P; Picci, P

    2007-09-01

    In recent years, classification of soft-tissue sarcomas (STS) has improved with cytogenetic analyses, but their clinical behavior is still not easily predictable. The aim of this study was to detect alterations in the urokinase-type plasminogen system, involved in tumor growth and invasion, by comparing mRNA levels of its components with those of paired normal tissues, and relating them with patient clinical course. Real-time PCR was performed on human STS cell lines and tissues from highly malignant STS, including leiomyosarcomas and malignant fibrous histiocytomas, to evaluate the expression of urokinase-type plasminogen activator (uPA), uPA receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1). Immunohistochemistry of gene products was also performed. Median mRNA values of all genes studied were higher in tumors than in paired normal tissues. In agreement with data on STS cell lines, significant up-regulation for uPA and PAI-1 genes compared to reference values was seen. Moreover, different levels of expression were related to histotype and metastatic phenotype. There was accordance between uPA mRNA and protein expression, while immunodetection of PAI-1 product was weak and scattered. Clearly, the controversial role of PAI-1 protein requires further biological analyses, but evident involvement of uPA/PAI-1 gene overexpression in STS malignancy may highlight a molecular defect useful in discriminating STS high-risk patients.

  10. Altered Intermittent Rhythmic Delta and Theta Activity in the Electroencephalographies of High Functioning Adult Patients with Autism Spectrum Disorder

    Science.gov (United States)

    Endres, Dominique; Maier, Simon; Feige, Bernd; Posielski, Nicole A.; Nickel, Kathrin; Ebert, Dieter; Riedel, Andreas; Philipsen, Alexandra; Perlov, Evgeniy; Tebartz van Elst, Ludger

    2017-01-01

    Background: Autism spectrum disorder (ASD) is often associated with epilepsy. Previous studies have also shown increased rates of electroencephalographic (EEG) alteration in ASD patients without epilepsy. The aim of this study was to compare the rate of intermittent rhythmic delta and theta activity (IRDA/IRTA) events between high-functioning adult patients with ASD and matched healthy controls. Materials and Methods: Routine EEG records of 19 ASD patients and 19 matched controls were screened for IRDA/IRTA using a fully data driven analysis with fixed thresholds. IRDA/IRTA rates before and after hyperventilation (HV) as well as the HV-induced difference in IRDA/IRTA rates (HV difference) were analyzed. For inter-group measures, we used the Wilcoxon rank sum test. Results: Significantly increased HV difference was detected in the ASD group (p = 0.0497). However, the groups showed no difference in IRDA/IRTA rates before HV (p = 0.564) and after HV (p = 0.163). Conclusions: The lack of any group differences regarding IRDA/IRTA before HV might be related to the fact that we only studied non-secondary high-functioning autism in a small sample of epilepsy-free adult patients. A significantly increased HV difference might be regarded as a marker of subtle neuronal network instability possibly causing short-term disturbances via local area network inhibition and long-term effects via epileptic encephalopathy. PMID:28265243

  11. Wheat leaf lipids during heat stress: I. High day and night temperatures result in major lipid alterations.

    Science.gov (United States)

    Narayanan, Sruthi; Tamura, Pamela J; Roth, Mary R; Prasad, P V Vara; Welti, Ruth

    2016-04-01

    Understanding how wheat (Triticum aestivum L.) plants under high temperature (HT) regulate lipid composition is critical to developing climate-resilient varieties. We measured 165 glycerolipids and sterol derivatives under optimum and high day and night temperatures in wheat leaves using electrospray ionization-tandem mass spectrometry. Levels of polar lipid fatty acyl chain unsaturation were lower in both heat-tolerant genotype Ventnor and susceptible genotype Karl 92 under HT, compared with optimum temperature. The lower unsaturation was predominantly because of lower levels of 18:3 acyl chains and higher levels of 18:1 and 16:0 acyl chains. Levels of 18:3-containing triacylglycerols increased threefold/more under HT, consistent with their possible role in sequestering fatty acids during membrane lipid remodelling. Phospholipids containing odd-numbered or oxidized acyl chains accumulated in leaves under HT. Sterol glycosides (SG) and 16:0-acylated sterol glycosides (ASG) were higher under HT than optimum temperatures. Ventnor had lower amounts of phospholipids with oxidized acyl chains under HT and higher amounts of SG and 16:0-ASG than Karl 92. Taken together, the data demonstrate that wheat leaf lipid composition is altered by HT, in which some lipids are particularly responsive to HT, and that two wheat genotypes, chosen for their differing physiological responses to HT, differ in lipid profile under HT.

  12. In vivo induction of antioxidant response and oxidative stress associated with genotoxicity and histopathological alteration in two commercial fish species due to heavy metals exposure in northern India (Kali) river.

    Science.gov (United States)

    Fatima, Mahino; Usmani, Nazura; Firdaus, Fakiha; Zafeer, Mohammad Faraz; Ahmad, Shafeeque; Akhtar, Kafil; Dawar Husain, S M; Ahmad, Mir Hilal; Anis, Ehraz; Mobarak Hossain, M

    2015-01-01

    Heavy metals can significantly bioaccumulate in fish tissues. The step wise mechanism of heavy metal toxicities on fish health is still limited. The present study assessed the tissue-specific antioxidant response and oxidative stress biomarkers of commercially important fish species namely, Channa striatus and Heteropneustes fossilis inhabiting Kali River of northern India where heavy-metal load is beyond the World Health Organisation - maximum permissible limits. Heavy metals chromium (Cr), nickel (Ni), lead (Pb) and cadmium (Cd) were elevated in both fish species compared to recommended values of the Federal Environmental Protection Agency (FEPA), 1999 for edible fishes. Reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CATA) activities in all tissues (brachial, neural, renal and hepatic) were altered. Cellular lipid and protein compromisation in both fishes induced by heavy metals was determined by lipid peroxidation (LPO) and protein carbonylation (PC) assays. Micronucleus (MN) test of erythrocytes and comet assay of liver cells confirmed genotoxicity. Histopathology of the liver, kidney and brain of affected fishes was distorted significantly with its reference fishes thereby affecting the quality and quantity of these fish stocks. This raises a serious concern as these fishes are consumed by the local population which would ultimately affect human health.

  13. Environmental flow assessment for river Trebizat, BiH

    Science.gov (United States)

    Smolar-Zvanut, N.; Kupusovic, E.; Vucijak, B.; Mijatovic, A.; Grizelj, Z.; Antonelli, F.

    2009-04-01

    The alteration of the water flow downstream of dams is one of the most stressful factors influencing the aquatic and riverine ecosystem. The environmental flow assessment is a tool for finding the balance between water use by humans and nature and ensuring a long-term and good quality water supply both for human purposes and for ecosystems. In 2007/08 WWF has implemented a project in the Neretva basin (Bosnia and Herzegovina) with a focus on environmental flow evaluation for the river Trebizat, located in the western region of Herzegovina. The water regime of the Trebizat river is affected by the abstraction of its water for hydropower plants, irrigation and fish farming not to mention pollution problems. The Trebizat river flows through an area of remarkable ecological value hosting also protected areas (the travertine-formation around Kravice waterfall). The main aim of this paper is to present the results of the application of a methodology for environmental flow assessment, namely the GEP methodology (guaranteed ecological flow). It belongs to the category of hydrological environmental flow assessment methods and the test was done to assess the environmental flow in the river Trebizat. Using existing hydrological data as well as samples specifically collected on the field, the environmental flow was assessed applying the GEP methodology. Additionally, instream ecological values and critical parameters for environmental flow assessment were evaluated. The area was assessed in terms of its geography, climate conditions, historic heritage of the river, demography, geology of the river and its tributaries, river hydrology and morphology, ecological characteristics, river pollution, river use and river management. At five selected sampling sites along the Trebizat river, additional data on macrophytes, phytobenthos and physico-chemical parameters were collected and analysed. Although there have been many negative impacts in recent years on the Trebizat river, the

  14. SOLUBILITY OF URANIUM AND PLUTONIUM IN ALKALINE SAVANNAH RIVER SITE HIGH LEVEL WASTE SOLUTIONS

    Energy Technology Data Exchange (ETDEWEB)

    King, W.; Hobbs, D.; Wilmarth, B.; Edwards, T.

    2010-03-10

    Five actual Savannah River Site tank waste samples and three chemically-modified samples were tested to determine solubility limits for uranium and plutonium over a one year time period. Observed final uranium concentrations ranged from 7 mg U/L to 4.5 g U/L. Final plutonium concentrations ranged from 4 {micro}g Pu/L to 12 mg Pu/L. Actinide carbonate complexation is believed to result in the dramatic solubility increases observed for one sample over long time periods. Clarkeite, NaUO{sub 2}(O)OH {center_dot} H{sub 2}O, was found to be the dominant uranium solid phase in equilibrium with the waste supernate in most cases.

  15. DEVELOPMENT OF AN IMPROVED SODIUM TITANATE FOR THE PRETREATMENT OF HIGH LEVEL NUCLEAR WASTE AT THE SAVANNAH RIVER SITE

    Energy Technology Data Exchange (ETDEWEB)

    Hobbs, D

    2007-11-15

    High-level nuclear waste produced from fuel reprocessing operations at the Savannah River Site (SRS) requires pretreatment to remove Cs-137, Sr-90 and alpha-emitting radionuclides (i.e., actinides) prior to disposal onsite as low level waste. Separation processes planned at SRS include sorption of Sr-90 and alpha-emitting radionuclides onto monosodium titanate (MST) and caustic side solvent extraction, for {sup 137}Cs removal. The predominant alpha-emitting radionuclides in the highly alkaline waste solutions include plutonium isotopes Pu-238, Pu-239 and Pu-240. This paper describes recent results to produce an improved sodium titanate material that exhibits increased removal kinetics and capacity for Sr-90 and alpha-emitting radionuclides compared to the baseline MST material.

  16. DEVELOPMENT OF AN IMPROVED SODIUM TITANATE FOR THE PRETREATMENT OF HIGH LEVEL NUCLEAR WASTE AT THE SAVANNAH RIVER SITE

    Energy Technology Data Exchange (ETDEWEB)

    Hobbs D. T.; Poirier, M. R.; Barnes, M. J.; Stallings, M. E.; Nyman, M. D.

    2005-11-22

    High-level nuclear waste produced from fuel reprocessing operations at the Savannah River Site (SRS) requires pretreatment to remove {sup 137}Cs, {sup 90}Sr and alpha-emitting radionuclides (i.e., actinides) prior to disposal onsite as low level waste. Separation processes planned at SRS include caustic side solvent extraction, for {sup 137}Cs removal, and sorption of {sup 90}Sr and alpha-emitting radionuclides onto monosodium titanate (MST). The predominant alpha-emitting radionuclides in the highly alkaline waste solutions include plutonium isotopes {sup 238}Pu, {sup 239}Pu and {sup 240}Pu. This paper describes recent results to produce an improved sodium titanate material that exhibits increased removal kinetics and capacity for {sup 90}Sr and alpha-emitting radionuclides compared to the baseline MST material.

  17. Performance of double-layer biofilter packed with coal fly ash ceramic granules in treating highly polluted river water.

    Science.gov (United States)

    Jing, Zhaoqian; Li, Yu-You; Cao, Shiwei; Liu, Yuyu

    2012-09-01

    To improve trickling filters' denitrification efficiency, a biofilter with a trickling upper layer and a submerged lower layer was developed and applied in treating highly polluted river water. It was packed with porous coal fly ash ceramic granules. Its start-up characteristics, influence of hydraulic loading rates (HLR), carbon/nitrogen (C/N) ratio and filter depth on pollutants removal were investigated. The results indicated this biofilter was started quickly in 16 days with river sediment as inoculum. Alternating nitrification and denitrification were achieved when water flowed downwards. COD and nitrogen were mainly removed in the upper layer and the lower layer, respectively. With HLR of 4.0-5.0m(3)/(m(2)d), chemical oxygen demand (COD), ammonium (NH(4)(+)-N) and total nitrogen (TN) in the effluent were below 50, 5 and 15 mg/L, respectively. This biofilter removed more than 80% of COD, 85% of NH(4)(+)-N and 60% of TN with C/N ratios ranging from 6 to 10.

  18. Assessing subaqueous mudslide hazard on the Mississippi River delta front, Part 2: Insights revealed through high-resolution geophysical surveying

    Science.gov (United States)

    Obelcz, J.; Xu, K.; Bentley, S. J.; Georgiou, I. Y.; Maloney, J. M.; Miner, M. D.; Hanegan, K.; Keller, G.

    2014-12-01

    The northern Gulf of Mexico, including the subaqueous Mississippi River delta front (MRDF), has been productive for oil and gas development since the early 1900s. In 1969 cyclic seafloor wave loading associated with the passage of Hurricane Camille triggered subaqueous mudflows across the MRDF, destroying several offshore oil platforms. This incident spurred geophysical and geotechnical studies of the MRDF, which found that the delta front is prone to mass failures on gentle gradients (survey area can be classified into four primary sedimentary facies: mudflow gullies, mudflow lobes, undisturbed prodelta, and undisturbed delta front. Subbottom profiles reveal extensive biogenic gas from 20 to about 80 m water depths on the delta front; sidescan data show a variety of bottleneck slides, mudflow gullies and mudflow noses. Previous studies have attempted to constrain the periodicity and magnitude of subaqueous mudslides on the MRDF. However, large age gaps and varied resolution between datasets result in ambiguity regarding the cause and magnitude of observed bathymetric changes. We present high-temporal resolution MRDF bathymetric variations from 2005 (post Hurricane Katrina), 2009 (relatively quiescent storm period), and 2014 (post 2011 Mississippi River flood). These data yield better magnitude and timing estimates of mass movements. This exercise represents a first step towards (1) assembling a comprehensive geologic dataset upon which future MRDF geohazard assessments can be founded, and (2) understanding the dynamics of a massive passive margin deltaic lobe entering a phase of decline.

  19. High Altitude Increases Alteration in Maximal Torque but Not in Rapid Torque Development in Knee Extensors after Repeated Treadmill Sprinting

    Science.gov (United States)

    Girard, Olivier; Brocherie, Franck; Millet, Grégoire P.

    2016-01-01

    We assessed knee extensor neuromuscular adjustments following repeated treadmill sprints in different normobaric hypoxia conditions, with special reference to rapid muscle torque production capacity. Thirteen team- and racquet-sport athletes undertook 8 × 5-s “all-out” sprints (passive recovery = 25 s) on a non-motorized treadmill in normoxia (NM; FiO2 = 20.9%), at low (LA; FiO2 = 16.8%) and high (HA; FiO2 = 13.3%) normobaric hypoxia (simulated altitudes of ~1800 m and ~3600 m, respectively). Explosive (~1 s; “fast” instruction) and maximal (~5 s; “hard” instruction) voluntary isometric contractions (MVC) of the knee extensors (KE), with concurrent electromyographic (EMG) activity recordings of the vastus lateralis (VL) and rectus femoris (RF) muscles, were performed before and 1-min post-exercise. Rate of torque development (RTD) and EMG (i.e., Root Mean Square or RMS) rise from 0 to 30, −50, −100, and −200 ms were recorded, and were also normalized to maximal torque and EMG values, respectively. Distance covered during the first 5-s sprint was similar (P > 0.05) in all conditions. A larger (P sprint decrement score and a shorter (P sprints occurred in HA (−8 ± 4% and 178 ± 11 m) but not in LA (−7 ± 3% and 181 ± 10 m) compared to NM (−5 ± 2% and 183 ± 9 m). Compared to NM (−9 ± 7%), a larger (P 0.05). Irrespectively of condition (P > 0.05), peak RTD (−6 ± 11%; P 0.05), whereas it increased (P repeated-sprint ability and post-exercise MVC decrease were greater at high altitude than in normoxia or at low altitude. However, the post-exercise alterations in RTD were similar between normoxia and low-to-high hypoxia. PMID:27014095

  20. Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows.

    Directory of Open Access Journals (Sweden)

    Guozhong Dong

    Full Text Available The objective of this study was to investigate the effects of feeding a high-concentrate corn straw (HCS diet (65% concentrate+35% corn straw on the epigenetic changes in the mammary tissue of dairy cows in comparison with a low-concentrate corn straw (LCS diet (46% concentrate+54% corn straw and with a low-concentrate mixed forage (LMF diet (46% concentrate+54% mixed forage.Multiparous mid-lactation Chinese Holstein cows were fed one of these three diets for 6 weeks, at which time blood samples and mammary tissue samples were collected. Mammary arterial and venous blood samples were analyzed for lipopolysaccharide (LPS concentrations while mammary tissue samples were assayed for histone H3 acetylation and the methylation of specific genes associated with fat and protein synthesis.Extraction of histones and quantification of histone H3 acetylation revealed that acetylation was significantly reduced in cows fed the HCS diet, as compared with cows fed the LCS diet. Cows fed the HCS diet had significantly higher LPS concentrations in the mammary arterial blood, as compared with cows fed the LCS diet. We found that the extent of histone H3 acetylation was negatively correlated with LPS concentrations. The methylation of the stearoyl-coenzyme A desaturase gene associated with milk fat synthesis was increased in cows fed the HCS diet. By contrast, methylation of the gene encoding the signal transducer and activator of transcription 5A was reduced in cows fed the HCS diet, suggesting that feeding a high-concentrate corn straw diet may alter the methylation of specific genes involved in fat and protein synthesis in the mammary tissue of dairy cows.Feeding the high-concentrate diet induced epigenetic changes in the mammary tissues of dairy cows, possibly through effecting the release of differing amounts of LPS into the mammary blood.

  1. River engineering

    NARCIS (Netherlands)

    De Vries, M.

    1993-01-01

    One dimension models - basic eauations, analytical models, numberical models. One dimensional models -suspended load, roughness and resistance of river beds. Solving river problems - tools, flood mitigation, bank protection.

  2. Short-term variations of phytoplankton communities in response to anthropogenic stressors in a highly altered temperate estuary

    Science.gov (United States)

    Sin, Yongsik; Jeong, Byungkwan

    2015-04-01

    Data for phytoplankton size classes, taxonomy, and water properties were collected through an episodic freshwater discharge event (4 days) in the temperate Youngsan River estuary, which is highly disturbed by manually regulated inputs of freshwater from a sea dike, to investigate the effects of an acute change in anthropogenic stressors on the short-term dynamics of phytoplankton and their surrounding environments. The salinity of the well-mixed saline water (33.2-33.5) decreased to as low as 4.0 and water temperature increased to 24.0 °C during the freshwater discharge, resulting in a stratified water column in the upper region of the estuary. During the discharge, chlorophyll a (chl a) concentrations increased to as much as 15.66 μg L-1 with micro-sized phytoplankton being dominant due to the presence of micro-sized freshwater phytoplankton, mostly Aulacoseira ambigua (98% in cell abundance), transported from the reservoir. Primary production decreased to as little as 87.9 mg C m-2 d-1, although nutrients such as NO2- + NO3- were supplied by the freshwater inputs of the discharge. Following the discharge, dinoflagellate blooms, dominated by Heterocapsa sp. (>88%), a nano-sized red tide species, developed in the upper regions of the estuary with peaks in chl a concentrations reaching as high as 30.33 μg L-1. Another red tide species, Prorocentrum micans, was also dominant in the estuary, suggesting that harmful algal blooms (HABs) are associated with anthropogenic stressors related to the freshwater inputs. The Shannon diversity index decreased to 0.18 while the Simpson dominance index increased to 0.94 during the discharge, but the diversity increased again following the discharge. The phytoplankton communities and diversity changed along the salinity gradient, corresponding to an "ecocline" pattern. The results of multivariate statistical analysis suggested that phytoplankton species and size structure were controlled mainly by salinity, water temperature

  3. Assessment of Missouri River floodplain invertebrates during historic inundation: implications for river restoration

    Directory of Open Access Journals (Sweden)

    Gosch N.J.C.

    2014-01-01

    Full Text Available Floodplain connectivity is important to aquatic organisms in large rivers. Anthropogenic alterations regulating the Missouri River have limited connectivity and negatively affected native fauna. Determining the biological response to rare inundation events may be important when considering potential restoration options on a regulated river; thus, we assessed benthic invertebrate and zooplankton communities at three floodplain sites during a historic Missouri River high-water event. Chironomid larvae dominated during most sampling trips and densities were often highest during initial sampling trips with lower densities as high water persisted. Similar trends were evident for rotifer, cladoceran, and copepod densities. Nonmetric multidimensional scaling also showed relatively high dissimilarity of densities between early and late sampling trips for benthic invertebrate and zooplankton communities. As such, short-term inundation may be more beneficial to Missouri River benthic invertebrate (mainly chironomid larvae and zooplankton production than more prolonged inundation lasting a month or more. Furthermore, restoration projects may be designed at elevations allowing more short-term inundation, which would likely benefit native fishes with additional spawning, nursery, and foraging habitat. Levee setbacks may be an effective restoration option for increasing the amount of habitat available for short-term inundation while potentially providing socioeconomic, flood-risk reduction benefits by enhancing flow conveyance.

  4. Effects of three high-flow experiments on the Colorado River ecosystem downstream from Glen Canyon Dam, Arizona

    Science.gov (United States)

    Melis, Theodore S.

    2011-01-01

    Three high-flow experiments (HFEs) were conducted by the U.S. Department of the Interior at Glen Canyon Dam, Arizona, in March 1996, November 2004, and March 2008. These experiments, also known as artificial or controlled floods, were large-volume, scheduled releases of water from Glen Canyon Dam that were designed to mimic some aspects of pre-dam Colorado River seasonal flooding. The goal of these experiments was to determine whether high flows could be used to benefit important physical and biological resources in Glen Canyon National Recreation Area and Grand Canyon National Park that had been affected by the operation of Glen Canyon Dam. Efforts such as HFEs that seek to maintain and restore downstream resources are undertaken by the U.S. Department of the Interior under the auspices of the Grand Canyon Protection Act of 1992 (GCPA; title XVIII, secs. 1801-1809, of Public Law 102-575). Scientists conducted a wide range of monitoring and research activities before, during, and after the experiments. Initially, research efforts focused on whether HFEs could be used to rebuild and maintain Grand Canyon sandbars, which provide camping beaches for hikers and whitewater rafters, create habitats potentially used by native fish and other wildlife, and are the source of windborne sand that may help to protect some archaeological resources from weathering and erosion. As scientists gained a better understanding of how HFEs affect the physical environment, research efforts expanded to include additional investigations about the effects of HFEs on biological resources, such as native fishes, nonnative sports fishes, riverside vegetation, and the aquatic food web. The chapters that follow summarize and synthesize for decisionmakers and the public what has been learned about HFEs to provide a framework for implementing similar future experiments. This report is a product of the Glen Canyon Dam Adaptive Management Program (GCDAMP), a Federal initiative authorized to ensure

  5. Climate change and river ecosystems: protection and adaptation options.

    Science.gov (United States)

    Palmer, Margaret A; Lettenmaier, Dennis P; Poff, N Leroy; Postel, Sandra L; Richter, Brian; Warner, Richard

    2009-12-01

    Rivers provide a special suite of goods and services valued highly by the public that are inextricably linked to their flow dynamics and the interaction of flow with the landscape. Yet most rivers are within watersheds that are stressed to some extent by human activities including development, dams, or extractive uses. Climate change will add to and magnify risks that are already present through its potential to alter rainfall, temperature, runoff patterns, and to disrupt biological communities and sever ecological linkages. We provide an overview of the predicted impacts based on published studies to date, discuss both reactive and proactive management responses, and outline six categories of management actions that will contribute substantially to the protection of valuable river assets. To be effective, management must be place-based focusing on local watershed scales that are most relevant to management scales. The first priority should be enhancing environmental monitoring of changes and river responses coupled with the development of local scenario-building exercises that take land use and water use into account. Protection of a greater number of rivers and riparian corridors is essential, as is conjunctive groundwater/surface water management. This will require collaborations among multiple partners in the respective river basins and wise land use planning to minimize additional development in watersheds with valued rivers. Ensuring environmental flows by purchasing or leasing water rights and/or altering reservoir release patterns will be needed for many rivers. Implementing restoration projects proactively can be used to protect existing resources so that expensive reactive restoration to repair damage associated with a changing climate is minimized. Special attention should be given to diversifying and replicating habitats of special importance and to monitoring populations at high risk or of special value so that management interventions can occur if the

  6. A chronic high fat diet alters the homologous and heterologous control of appetite regulating peptide receptor expression.

    Science.gov (United States)

    Kentish, Stephen J; Wittert, Gary A; Blackshaw, L Ashley; Page, Amanda J

    2013-08-01

    Leptin, ghrelin and neuropeptide W (NPW) modulate vagal afferent activity, which may underlie their appetite regulatory actions. High fat diet (HFD)-induced obesity induces changes in the plasma levels of these peptides and alters the expression of receptors on vagal afferents. We investigated homologous and heterologous receptor regulation by leptin, ghrelin and NPW. Mice were fed (12 weeks) a standard laboratory diet (SLD) or HFD. Nodose ganglia were cultured overnight in the presence or absence of each peptide. Leptin (LepR), ghrelin (GHS-R), NPW (GPR7) and cholecystokinin type-1 (CCK1R) receptor mRNA, and the plasma leptin, ghrelin and NPW levels were measured. SLD: leptin reduced LepR, GPR7, increased GHS-R and CCK1R mRNA; ghrelin increased LepR, GPR7, CCK1R, and decreased GHS-R. HFD: leptin decreased GHS-R and GPR7, ghrelin increased GHS-R and GPR7. NPW decreased all receptors except GPR7 which increased with HFD. Plasma leptin was higher and NPW lower in HFD. Thus, HFD-induced obesity disrupts inter-regulation of appetite regulatory receptors in vagal afferents.

  7. Altered depth of the olfactory sulcus in ultra high-risk individuals and patients with psychotic disorders.

    Science.gov (United States)

    Takahashi, Tsutomu; Wood, Stephen J; Yung, Alison R; Nelson, Barnaby; Lin, Ashleigh; Yücel, Murat; Phillips, Lisa J; Nakamura, Yumiko; Suzuki, Michio; Brewer, Warrick J; Proffitt, Tina M; McGorry, Patrick D; Velakoulis, Dennis; Pantelis, Christos

    2014-03-01

    A shallow olfactory sulcus has been reported in schizophrenia, possibly reflecting abnormal forebrain development during early gestation. However, it remains unclear whether this anomaly exists prior to the onset of psychosis and/or differs according to illness stage. In the current study, magnetic resonance imaging was used to investigate the length and depth of the olfactory sulcus in 135 ultra high-risk (UHR) individuals [of whom 52 later developed psychosis (UHR-P) and 83 did not (UHR-NP)], 162 patients with first-episode psychosis (FEP), 89 patients with chronic schizophrenia, and 87 healthy controls. While there was no group difference in the length of the sulcus, UHR-P subjects had significantly shallower olfactory sulcus at baseline as compared with UHR-NP and control subjects. The depth of this sulcus became increasingly more superficial as one moved from UHR-P subjects to FEP patients to chronic schizophrenia patients. Finally, the depth of the olfactory sulcus in the UHR-P subjects was negatively correlated with the severity of negative symptoms. These findings suggest that the altered depth of the olfactory sulcus, which exists before psychosis onset, could be predictive of transition to psychosis, but also suggest ongoing changes of the sulcus morphology during the course of the illness.

  8. Examining the spatial and temporal variation of groundwater inflows to a valley-to-floodplain river using 222Rn, geochemistry and river discharge: the Ovens River, southeast Australia

    Directory of Open Access Journals (Sweden)

    M. C. L. Yu

    2013-04-01

    Full Text Available Radon (222Rn and major ion geochemistry were used to define and quantify the catchment-scale river-aquifer interactions along the Ovens River in the southeast Murray-Darling Basin, Victoria, Australia, between September 2009 and October 2011. The Ovens River is characterized by the transition from a single channel river residing within a mountain valley in the upper catchment to a multi-channel meandering river on flat alluvial plains in the lower catchment. Overall, the river is dominated by gaining reaches, receiving groundwater from both alluvial and basement aquifers. The distribution of gaining and losing reaches is governed by catchment morphology and lithology. In the upper catchment, rapid groundwater recharge through sediments that have high hydraulic conductivities in a narrow valley produces higher baseflow to the river during wet (high flow periods as a result of hydraulic loading. In the lower catchment, the open and flat alluvial plains, lower rainfall and finer-gained sediments reduce the magnitude and variability of hydraulic gradient between the aquifer and the river, producing lower and constant groundwater inflow. With a small difference between the water table and the river height, small changes in river height or in groundwater level can result fluctuating gaining and losing behaviour along the river. The middle catchment represents a transition in river-aquifer interactions from upper to lower catchment. High baseflow in some parts of the middle and lower catchments is caused by groundwater flow over basement highs. Mass balance calculations based on 222Rn activities indicate that groundwater inflow is 4–22% of total flow with higher baseflow occurring in high flow periods. Uncertainties in gas exchange coefficient and 222Rn activities of groundwater alter the calculated groundwater inflow to 3–35%. Ignoring hyporheic exchange appears not to have a significant impact on the total groundwater estimates. In comparison to

  9. Investigation of the Spatial Patterns of Velocity and Bed Morphology Within Actively Evolving Neck Cutoffs on a Highly Sinuous River

    Science.gov (United States)

    Richards, D.; Konsoer, K. M.; Turnipseed, C.; Willson, C. S.

    2016-12-01

    Meander bend cutoffs and oxbow lakes are essential and ubiquitous features of meandering river floodplains. Yet, the processes involved in the evolution of an elongated bend to neck cutoff and oxbow lake are incompletely understood. Five neck cutoffs occurring on the White River, Arkansas, with two types of planform geometries, and one elongate meander loop prior to cutoff were examined for this study. Three-dimensional velocities were obtained using an acoustic Doppler current profiler (ADCP), and high-resolution channel bathymetry was collected using multi-beam echo sounder (MBES). Results show similar patterns of acceleration of velocity with nearly 180 degree redirection of the flow through the cutoff, strong advection of high momentum toward the far bank, and multiple zones of flow separation and recirculation. This pattern of velocity leads to increased bank erosion rates on the downstream bank opposite of the cutoff, bar formation downstream of the cutoff along the adjacent banks, and relatively deep scour pools located within the region of flow acceleration immediately downstream of the cutoff. Analysis of high-resolution channel bathymetry show a wide range in bedform morphology, the presence of amalgamating unit bars, and a lack of sediment accumulation within the entrance and exit to the abandoned meander loops. The findings from this investigation reveal that the evolution of these neck cutoffs do not conform to current conceptual models of meander cutoff and oxbow lake formation, suggesting that cutoff location, planform geometry, and length of channel removed are important factors in determining planform evolution and the hydrologic connectivity of the abandoned bend to the active channel.

  10. Evaluating long-term trends in mean- and high- river flows using a network of reference stations in Ireland

    Science.gov (United States)

    Wilby, R. L.; Murphy, C.; Harrigan, S.; Hall, J.

    2012-12-01

    This paper describes the development of a reference hydrometric network for Ireland established primarily for the detection of climate driven trends in mean and high river flows. Thirty-five stations were identified for inclusion in the network plus a further 8 from the UK Benchmark Network. Their average record length is 40 years with a minimum of 28 and maximum of 63 years. Time series were derived for eight river flow indices: annual and seasonal mean flows and the annual maximum 1-, 10- and 30-day flows. Mann Kendall and Theil Sen statistics were applied to all indices using fixed and variable start/end dates. Trends in the winter mean are found to be highly dependent on the chosen period of analysis with the longest records showing increased flows. Contrary to expectations (of regional climate change scenarios), increases are also evident for long-term summer mean flows. High flow metrics exhibit positive and persistent trends that are less affected by inter-annual variability and period of record. Overall, there is strong spatial coherence in these patterns of change, linked to temporal variations in precipitation. Our results highlight the dangers of using conventional fixed periods such as 1961-1990 for trend detection, recognising that there is always a trade-off between record length, density of the network, and geographic coverage. Furthermore, outliers at the beginning of the record can be an artefact of the original motivation for installing the gauging station(s). In this case, water resource concerns during a markedly dry period in the mid-1970s favour positive trends over subsequent decades. Future work will focus on detection times for climate change signals and the identification of sentinel sites for discerning early signs of anthropogenic climate change across Ireland. Broader lessons for monitoring and detection of anthropogenic climate change signals will also be distilled.

  11. A geomorphologist's dream come true: synoptic high resolution river bathymetry with the latest generation of airborne dual wavelength lidar

    Science.gov (United States)

    Lague, Dimitri; Launeau, Patrick; Michon, Cyril; Gouraud, Emmanuel; Juge, Cyril; Gentile, William; Hubert-Moy, Laurence; Crave, Alain

    2016-04-01

    Airborne, terrestrial lidar and Structure From Motion have dramatically changed our approach of geomorphology, from low density/precision data, to a wealth of data with a precision adequate to actually measure topographic change across multiple scales, and its relation to vegetation. Yet, an important limitation in the context of fluvial geomorphology has been the inability of these techniques to penetrate water due to the use of NIR laser wavelengths or to the complexity of accounting for water refraction in SFM. Coastal bathymetric systems using a green lidar can penetrate clear water up to 50 m but have a resolution too coarse and deployment costs that are prohibitive for fluvial research and management. After early prototypes of narrow aperture green lidar (e.g., EEARL NASA), major lidar manufacturer are now releasing dual wavelength laser system that offer water penetration consistent with shallow fluvial bathymetry at very high resolution (> 10 pts/m²) and deployment costs that makes the technology, finally accessible. This offers unique opportunities to obtain synoptic high resolution, high precision data for academic research as well as for fluvial environment management (flood risk mapping, navigability,…). In this presentation, we report on the deployment of the latest generation Teledyne-Optech Titan dual-wavelength lidar (1064 nm + 532 nm) owned by the University of Nantes and Rennes. The instrument has been deployed over several fluvial and lacustrine environments in France. We present results and recommendation on how to optimize the bathymetric cover as a function of aerial and aquatic vegetation cover and the hydrology regime of the river. In the surveyed rivers, the penetration depth varies from 0.5 to 4 m with discrete echoes (i.e., onboard detection), heavily impacted by water clarity and bottom reflectance. Simple post-processing of the full waveform record allows to recover an additional 20 % depth. As for other lidar techniques, the main

  12. Meal pattern alterations associated with intermittent fasting for weight loss are normalized after high-fat diet re-feeding.

    Science.gov (United States)

    Gotthardt, Juliet D; Bello, Nicholas T

    2017-03-03

    Alternate day, intermittent fasting (IMF) can be an effective weight loss strategy. However, the effects of IMF on eating behaviors are not well characterized. We investigated the acute and residual effects of IMF for weight loss on meal patterns in adult obese male C57BL/6 mice. After 8weeks of ad libitum high-fat diet to induce diet-induced obesity (DIO), mice were either continued on ad libitum high-fat diet (HFD) or placed on one of 5 diet strategies for weight loss: IMF of high-fat diet (IMF-HFD), pair-fed to IMF-HFD group (PF-HFD), ad libitum low-fat diet (LFD), IMF of low-fat diet (IMF-LFD), or pair-fed to IMF-LFD group (PF-LFD). After the 4-week diet period, all groups were refed the high-fat diet for 6weeks. By the end of the diet period, all 5 groups had lost weight compared with HFD group, but after 6weeks of HFD re-feeding all groups had similar body weights. On (Day 2) of the diet period, IMF-HFD had greater first meal size and faster eating rate compared with HFD. Also, first meal duration was greater in LFD and IMF-LFD compared with HFD. At the end of the diet period (Day 28), the intermittent fasting groups (IMF-HFD and IMF-LFD) had greater first meal sizes and faster first meal eating rate compared with their respective ad libitum fed groups on similar diets (HFD and LFD). Also, average meal duration was longer on Day 28 in the low-fat diet groups (LFD and IMF-LFD) compared with high-fat diet groups (HFD and IMF-HFD). After 6weeks of HFD re-feeding (Day 70), there were no differences in meal patterns in groups that had previously experienced intermittent fasting compared with ad libitum fed groups. These findings suggest that meal patterns are only transiently altered during alternate day intermittent fasting for weight loss in obese male mice.

  13. Eight week exposure to a high sugar high fat diet results in adiposity gain and alterations in metabolic biomarkers in baboons (Papio hamadryas sp.

    Directory of Open Access Journals (Sweden)

    Tejero M Elizabeth

    2010-10-01

    Full Text Available Abstract Background Baboons (Papio hamadryas Sp. develop features of the cardiometabolic syndrome and represent a clinically-relevant animal model in which to study the aetiology of the disorder. To further evaluate the baboon as a model for the study of the cardiometabolic syndrome, we developed a high sugar high fat diet and hypothesized that it could be used to induce adiposity gain and affect associated circulating biomarkers. Methods We developed a diet enriched with monosaccharides and saturated fatty acids that was composed of solid and liquid energy sources. We provided a group of baboons (n = 9 ad libitum access to this diet for 8 weeks. Concurrently, a control group (n = 6 was maintained with ad libitum access to a low sugar low fat baseline diet and normal water for 8 weeks. Body composition was determined by dual-energy X-ray absorptiometry and circulating metabolic biomarkers were measured using standard methodology before and after the 8 week study period. Results Neither body composition nor circulating biomarkers changed in the control group. Following the 8 weeks, the intervention group had a significant increase in fat mass (1.71 ± 0.98 vs. 3.23 ± 1.70 kg, p = 0.004, triglyceride (55 ± 13 vs. 109 ± 67 mg/dL, p = 0.006,, and leptin (1.19 ± 1.40 vs. 3.29 ± 2.32 ng/mL, p = 0.001 and a decline in adiponectin concentrations (33530 ± 9744 vs. 23330 ± 7863 ng/mL, p = 0.002. Percentage haemoglobin A1C (4.0 ± 0.3 vs. 6.0 ± 1.4, p = 0.002 also increased in the intervention group. Conclusions Our findings indicate that when exposed to a high sugar high fat diet, young adult male baboons develop increased body fat and triglyceride concentrations, altered adipokine concentrations, and evidence of altered glucose metabolism. Our findings are in keeping with observations in humans and further demonstrate the potential utility of this highly clinically-relevant animal model for studying diet-induced metabolic dysregulation.

  14. Estimation of high resolution shallow water bathymetry via two-media-photogrammetry - a case study at the Pielach River

    Science.gov (United States)

    Wimmer, Michael; Mandlburger, Gottfried; Ressl, Camillo; Pfeifer, Norbert

    2016-04-01

    In our contribution, a photogrammetric approach for water depth estimation of a shallow water body is developed and applied to a gravel-bed river in order to evaluate the possibilities of passive optical remote sensing for high resolution bathymetry. While 2-media (air and water) photogrammetry has been described before, it was concentrated on reconstruction of individual points. Here, we take a different approach and aim at a dense surface description of the river bed as seen from the aerial image through the water column. In a first step, the influence of light refraction at the boundary between two media for photogrammetric point retrieval is assessed. The effect is theoretically investigated under varying conditions, i.e. the 3D point displacement caused by refraction is related to parameters such as water depth, image geometry et cetera. Especially the assumption of a plain, horizontal water surface does not hold in practice. Therefore, also the limitations of the theoretical model are determined by investigating, how water surface waves and the corresponding deviation of the surface normal vectors from vertical direction distort the results. In the second, practical part of the work, a refraction correction procedure is derived from the prior investigations and is embedded into the photogrammetric workflow. A full photogrammetric processing chain is applied to a set of aerial images of the pre-Alpine Pielach River in Lower Austria. The RGB images were taken simultaneously to an Airborne Laser Bathymetry (ALB) campaign providing high resolution reference data. Based on these images, a Digital Terrain Model is derived for the open as well as the submerged areas. Running through the procedure gives important insights about the possibilities of influencing the processing pipeline of commercial photogrammetric software packages in order to apply the developed refraction correction. Especially, the deviation from the epipolar constraint caused by refraction and the

  15. High Chromium Tolerant Bacterial Strains from Palar River Basin: Impact of Tannery Pollution

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    K. Sundar

    2010-04-01

    Full Text Available The basic survey study on tanneries and its pollution in the Palar river basin of Vellore District showed that it has been contaminated with heavy metals especially chromium and salts. This study is to improve our understanding to find the Cr contamination level and the ecology of heavy metal tolerance of the native bacterial flora of our study area. Chromium tolerant strains were isolated from contaminated sediments, water and effluents of various tanneries. The minimum and maximum concentration of chromium sediments was in the range of 47.4 and 682.4 mg/L, with an average of 306.285 mg/L in the study area. Sixty-eight chromium resistant bacterial strains were isolated and Maximum Tolerance Concentration (MTC studies have indicated that the tolerance concentrations of the isolates were in the range of 100-3300 mg/L. These bacterial isolates were also checked for their resistance to other heavy metals like Ni, Pb, Zn, Fe and Cd. Eighty percent of the isolates showed resistance to Ni, Pb, Zn, Fe at 100 ppm level and 45% had shown resistance to Cd. The isolates also had shown tolerance to salt (NaCl up to 9%. Significant note was found in the concentration of chromium and in the chromium tolerance ability of the bacteria in the study area and these chromium tolerance bacteria can be used as the indicator for the Cr contamination.

  16. High-impact hydrologic events and atmospheric rivers in California: An investigation using the NCEI Storm Events Database

    Science.gov (United States)

    Young, Allison M.; Skelly, Klint T.; Cordeira, Jason M.

    2017-04-01

    Atmospheric rivers (ARs) are long, narrow corridors of enhanced integrated water vapor and integrated vapor transport that can result in high-impact hydrologic events (HIHEs) including floods, flash floods, and debris flows. This study examined the relationship between HIHEs and ARs in California for 10 water years by using the National Centers for Environmental Information Storm Events Database and a catalog of landfalling ARs provided by Rutz et al. Results illustrated that HIHEs related to floods and debris flows are commonly associated with ARs during the cold season across Northern California, whereas HIHEs related to flash floods are commonly not associated with ARs during the warm season across Southern California. Composite analyses illustrated that HIHEs associated with landfalling ARs are associated with synoptic-scale flow patterns that support southwesterly water vapor flux that aligns favorably with California coastal topography to maximize upslope flow and orographic precipitation.

  17. SOLIDIFICATION TESTING FOR A HIGH ACTIVITY WASTESTREAM FROM THE SAVANNAH RIVER SITE USING GROUT AND GAMMA RADIATION SHEILDING MATERIALS - 10017

    Energy Technology Data Exchange (ETDEWEB)

    Burns, H.

    2009-11-10

    The U.S. Department of Energy (DOE) tasked MSE Technology Applications, Inc. (MSE) with evaluating grouts that include gamma radiation shielding materials to solidify surrogates of liquid aqueous radioactive wastes from across the DOE Complex. The Savannah River Site (SRS) identified a High Activity Waste (HAW) that will be treated and solidified at the Waste Solidification Building (WSB) for surrogate grout testing. The HAW, which is produced at the Mixed Oxide Fuel Fabrication Facility (MFFF), is an acidic aqueous wastestream generated by the alkaline treatment process and the aqueous purification process. The HAW surrogate was solidified using Portland cement with and without the inclusion of different gamma radiation shielding materials to determine the shielding material that is the most effective to attenuate gamma radiation for this application.

  18. Two new species of nematodes (Nematoda) from highly mineralized rivers of Lake El'ton basin, Russia.

    Science.gov (United States)

    Gusakov, Vladimir A; Gagarin, Vladimir G

    2016-09-05

    Two new nematode species, Mesodorylaimus rivalis sp. n. and Allodiplogaster media sp. n., from the highly mineralized rivers of the El'ton Lake basin (Russia) are described and illustrated from numerous mature females and males. Mesodorylaimus rivalis sp. n. is similar to M. vulvapapillatus Bagaturia & Eliava, 1966, but differs from it in the longer body, shorter spicules and longer female prerectum. Allodiplogaster media sp. n. resembles A. lupata (Shoshin, 1989) Kanzaki, Ragsdale & Giblin-Davis, 2014 and A. mordax (Shoshin, 1989) Kanzaki, Ragsdale & Giblin-Davis, 2014, but differs from the first species in having a shorter pharynx, shorter outer labial setae, longer spicules and different ratio between anterior and posterior pharynx sections, and from A. mordax in the thinner body, shorter pharynx and longer spicules.

  19. Extreme hydrological events and the influence of reservoirs in a highly regulated river basin of northeastern Spain

    Directory of Open Access Journals (Sweden)

    S.M. Vicente-Serrano

    2017-08-01

    New hydrological insights: Results reveal a general reduction in the occurrence of extreme precipitation events in the Segre basin from 1950 to 2013, which corresponded to a general reduction in high flows measured at various gauged stations across the basin. While this study demonstrates spatial differences in the decrease of streamflow between the headwaters and the lower parts of the basin, mainly associated with changes in river regulation, there was no reduction in the frequency of the extraordinary floods. Changes in water management practices in the basin have significantly impacted the frequency, duration, and severity of hydrological droughts downstream of the main dams, as a consequence of the intense water regulation to meet water demands for irrigation and livestock farms. Nonetheless, the hydrological response of the headwaters to these droughts differed markedly from that of the lower areas of the basin.

  20. Multi-scale evaluation of high-resolution multi-sensor blended global precipitation products over the Yangtze River

    Science.gov (United States)

    Li, Zhe; Yang, Dawen; Hong, Yang

    2013-09-01

    In the present study, four high-resolution multi-sensor blended precipitation products, TRMM Multisatellite Precipitation Analysis (TMPA) research product (3B42 V7) and near real-time product (3B42 RT), Climate Prediction Center MORPHing technique (CMORPH) and Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks (PERSIANN), are evaluated over the Yangtze River basin from April 2008 to March 2012 using the gauge data. This regional evaluation is performed at temporal scales ranging from annual to daily, based on a number of diagnostic statistics. Gauge adjustment greatly reduces the bias in 3B42 V7, a post real-time research product. Additionally, it helps the product maintain a stable skill level in winter. When additional indicators such as spatial correlation, Root Mean Square Error (RMSE), and Probability of Detection (POD) are considered, 3B42 V7 is not always superior to other products (especially CMORPH) at the daily scale. Among the near real-time datasets, 3B42 RT overestimates annual rainfall over the basin; CMORPH and PERSIANN underestimate it. In particular, the upper Yangtze always suffers from positive bias (>1 mm day-1) in the 3B42 RT dataset and negative bias (-0.2 to -1 mm day-1) in the CMORPH dataset. When seasonal scales are considered, CMORPH exhibits negative bias, mainly introduced during cold periods. The correlation between CMORPH and gauge data is the highest. On the contrary, the correlation between 3B42 RT and gauge data is more scattered; statistically, this results in lower bias. Finally, investigation of the probability distribution functions (PDFs) suggests that 3B42 V7 and 3B42 RT are consistently better at retrieving the PDFs in high-intensity events. Overall, this study provides useful information about the error characteristics associated with the four mainstream satellite precipitation products and their implications regarding hydrological applications over the Yangtze River basin.

  1. Comparison of Methods to Map and Measure River Terraces using High-Resolution Airborne LiDAR Data

    Science.gov (United States)

    Hopkins, A. J.; Snyder, N. P.

    2013-12-01

    Fluvial terraces are important recorders of land-use, climate, and tectonic history that form in both erosional and depositional landscapes and consist of a flat surface bounded by valley walls and a steep-sloping scarp adjacent to the river channel. Combining these defining characteristics with high-resolution digital elevation models (DEMs) derived from airborne light detection and ranging (lidar) surveys, several methods have been developed to identify and map terraces. The goals of this research are to compare some of these existing techniques and develop an objective approach to map terraces over entire watersheds using lidar DEMs. Additionally, we aim to quantify the thickness and volume of fill terrace deposits. Our preliminary application is to the Sheepscot River watershed, Maine, where strath and fill terraces are present and record Pleistocene deglaciation, Holocene eustatic forcing, and Anthropocene land-use change. We identify terraces along the longitudinal profile using an algorithm developed by Finnegan and Balco (2013), that computes the elevation frequency distribution at regularly spaced cross-sections normal to the channel. Next, we delineate terrace spatial extent using three separate methodologies: (1) image processing using Matlab, (2) feature classification algorithms developed by Wood (1996), and (3) image interpretation using manually placed points on known terraces to construct interpolated surfaces (Walter and Merritts, 2008). Lastly, we determine the thickness and volume of fill terrace sediments by subtracting an interpolated, adjacent water surface elevation from the defined terrace points. We compare our LiDAR-based results with field mapping, stratigraphic columns of terrace landforms, and ground penetrating radar over terrace surfaces. These findings suggest powerful new ways to rapidly analyze landscape history over large regions using high-resolution lidar DEMs, with less reliance on detailed and costly field data collection.

  2. The risk of river pollution due to washout from contaminated floodplain water bodies during periods of high magnitude floods

    Science.gov (United States)

    Lyubimova, T.; Lepikhin, A.; Parshakova, Ya.; Tiunov, A.

    2016-03-01

    The risk of river pollution due to washout (removal of pollutants) from contaminated floodplain water bodies (floodplain lakes and quarries whose origin is related to the large-scale mining of nonmetallic building materials in the floodplain zone) during high magnitude flood periods is analyzed using a combination of one-, two- and three-dimensional hydrodynamic modeling and in situ measurements. The modeling performed for the floodplain water bodies contaminated by N compounds shows that during large magnitude floods washout occurs. The washout process consists of two stages: an initial rapid stage lasting about two hours during which the upper (3-4 m thick) layer is washed out, followed by a second stage when the concentration of NH4-N in the floodplain water body remains nearly constant. The maximum contaminant concentration in the river in the vicinity of a water intake for drinking water located 21 km downstream is attained about 9 h from the beginning of the flood; concentration of NH4-N can reach values several times larger than acceptable concentration guidelines. The initial primary peak in contaminant concentration at the water intake is followed by a slight decrease in contaminant concentration; a second peak related to the contaminant transport through the inundated floodplain subsequently occurs, after which the concentration slowly decreases, reaching acceptable values after 30-40 h. Contaminated floodplain water bodies located near drinking water supply systems are not significant sources of contamination during small and moderate floods, but during high magnitude floods, they can become sources of water pollution. Operational measures that can decrease potential health risks are discussed.

  3. Developmental alterations and endocrine-disruptive responses in farmed Nile crocodiles (Crocodylus niloticus) exposed to contaminants from the Crocodile River, South Africa.

    Science.gov (United States)

    Arukwe, Augustine; Myburgh, Jan; Langberg, Håkon A; Adeogun, Aina O; Braa, Idunn Godal; Moeder, Monika; Schlenk, Daniel; Crago, Jordan Paul; Regoli, Francesco; Botha, Christo

    2016-04-01

    In the present study, the developmental (including fertility) and endocrine-disruptive effects in relation to chemical burden in male and female Nile crocodiles (Crocodylus niloticus), from a commercial crocodile farm in the Brits district, South Africa, exposed to various anthropogenic aquatic contaminants from the natural environment was investigated. Hepatic transcript levels for vitellogenin (Vtg), zona pellucida (ZP) and ERα (also in gonads) were analyzed using real-time PCR. Plasma estradiol-17β (E2), testosterone (T) and 11-ketotestosterone (11-KT) were analyzed using enzyme immunoassay. Gonadal aromatase and hepatic testosterone metabolism (6β-hydroxylase (6β-OHase)) were analyzed using biochemical methods. Overall, there is high and abnormal number (%) of infertile and banded eggs during the studied reproductive seasons, showing up to 57 and 34% of infertile eggs in the 2009/2010 and 2013/2014 seasons, respectively. In addition, the percentage of banded eggs ranged between 10 and 19% during the period of 2009-2014 seasons. While hepatic ERα, Vtg, ZP mRNA and testosterone 6β-OHase, were equally expressed in female and male crocodiles, gonadal ERα mRNA and aromatase activity were significantly higher in females compared to male crocodiles. On the other hand, plasma T and 11-KT levels were significantly higher in males, compared to female crocodiles. Principal component analysis (PCA) produced significant grouping that revealed correlative relationships between reproductive/endocrine-disruptive variables and liver contaminant burden, that further relates to measured contaminants in the natural environment. The overall results suggest that these captive pre-slaughter farm crocodiles exhibited responses to anthropogenic aquatic contaminants with potentially relevant consequences on key reproductive and endocrine pathways and these responses may be established as relevant species endocrine disruptor biomarkers of exposure and effects in this threatened

  4. Study of solid-phase extraction for the determination of sequestering agents in river water by high-performance liquid chromatography.

    Science.gov (United States)

    Kemmei, Tomoko; Kodama, Shuji; Muramoto, Tatsuya; Fujishima, Hironori; Yamamoto, Atsushi; Inoue, Yoshinori; Hayakawa, Kazuichi

    2009-02-13

    Anion-exchange solid-phase extraction accompanied with high-performance liquid chromatography has been developed for the determination of six kinds of aminopolycarboxylic acids (APCAs) in river water [N-(2-hydroxyethyl)ethylenediaminetriacetate (HEDTA), ethylenediaminetetraacetate (EDTA), 1,3-propanediaminetetraacetate (PDTA), diethylenetriaminepentaacetate (DTPA), 1,2-propanediaminetetraacetate (MeEDTA), and O,O'-bis(2-aminoethyl)ethyleneglycoltetraacetate (GEDTA)]. The enrichment of APCAs using an anion-exchange cartridge was successfully done by the removal of anions, which competed with APCAs in anion-exchange processes. Barium chloride solution was added to river water and the mixture was passed through On Guard II Ag and H cartridges and then a Bond Elut Jr.SAX cartridge to enrich APCAs. After elution, APCAs were analyzed on two reversed phase C30 columns connected in series and detected with ultraviolet detection. The enrichment using solid-phase extraction permitted the determination of APCAs in river water at concentrations as low as 1nM. Good recoveries (83-111%) were obtained for each APCA by the standard addition method on three river water samples with high accuracy (RSD 1.8-9.5%). Applying this method, two kinds of APCAs, EDTA and DTPA, were determined in samples from the Oyabe and Senbo Rivers in Japan.

  5. Potential of High Spatial and Temporal Ocean Color Satellite Data to Study the Dynamics of Suspended Particles in a Micro-Tidal River Plume

    Directory of Open Access Journals (Sweden)

    Anouck Ody

    2016-03-01

    Full Text Available Ocean color satellite sensors are powerful tools to study and monitor the dynamics of suspended particulate matter (SPM discharged by rivers in coastal waters. In this study, we test the capabilities of Landsat-8/Operational Land Imager (OLI, AQUA&TERRA/Moderate Resolution Imaging Spectroradiometer (MODIS and MSG-3/Spinning Enhanced Visible and Infrared Imager (SEVIRI sensors in terms of spectral, spatial and temporal resolutions to (i estimate the seawater reflectance signal and then SPM concentrations and (ii monitor the dynamics of SPM in the Rhône River plume characterized by moderately turbid surface waters in a micro-tidal sea. Consistent remote-sensing reflectance (Rrs values are retrieved in the red spectral bands of these four satellite sensors (median relative difference less than ~16% in turbid waters. By applying a regional algorithm developed from in situ data, these Rrs are used to estimate SPM concentrations in the Rhône river plume. The spatial resolution of OLI provides a detailed mapping of the SPM concentration from the downstream part of the river itself to the plume offshore limits with well defined small-scale turbidity features. Despite the low temporal resolution of OLI, this should allow to better understand the transport of terrestrial particles from rivers to the coastal ocean. These details are partly lost using MODIS coarser resolutions data but SPM concentration estimations are consistent, with an accuracy of about 1 to 3 g·m−3 in the river mouth and plume for spatial resolutions from 250 m to 1 km. The MODIS temporal resolution (2 images per day allows to capture the daily to monthly dynamics of the river plume. However, despite its micro-tidal environment, the Rhône River plume shows significant short-term (hourly variations, mainly controlled by wind and regional circulation, that MODIS temporal resolution failed to capture. On the contrary, the high temporal resolution of SEVIRI makes it a powerful tool to

  6. Clumped Isotope Verification of δ18O-Based Freshwater Mussel Shell Growth Chronology for a High-Resolution Climate and River Discharge Record

    Science.gov (United States)

    VanPlantinga, A.; Grossman, E. L.; Passey, B. H.; Randklev, C.

    2015-12-01

    Isotope profiles in freshwater mussel shells can be used to reconstruct climate, water source, and river discharge, but problems arise from variable water temperature and δ18O. To resolve this complexity and expand the application of isotope sclerochronology to the study of past river systems, we measured δ18O and Δ47 in two common freshwater mussel species from the Brazos River in Texas. To compare the environmental record with the shell record and develop a sclerochronology, weekly water temperature and δ18O data were collected from the Brazos River near College Station from January 2012 to August 2013. The river data reveal complex, irregular patterns for predicted aragonite δ18O. Comparing δ18O profiles from micromilled transects (70-200 µm increments) of coeval shell growth within and between shells yielded consistent patterns. Shell δ18O can be accurately matched to predicted δ18O, providing a chronology of shell growth. However, without a water temperature and δ18O record, interpreting a sclerochronology would be impossible. Shell Δ47 can potentially provide a seasonal chronology to verify the δ18O sclerochronology, which would be invaluable for the use of δ18O sclerochronology in historical and ancient shells. For Δ47 analyses, samples were taken at 0.5 mm resolution in presumed seasonal dark and light growth bands. Clumped temperatures range between 21 and 35 ± 4˚C (Henkes et al., 2013) and track the river temperature record, supporting the interpreted shell δ18O chronology. Shell Δ47-calculated water δ18O values range from -1.2 to 1.5 ± 0.9‰ and match river δ18O. High-resolution shell δ18O profiles combined with Δ47 temperatures can reconstruct a weekly history of water δ18O, and with the observed river discharge vs. water δ18O relation, produce a qualitative record of river discharge. These analytical techniques applied to a historical Brazos River mussel shell collected prior to dam construction reveal weekly records of

  7. Magmatic-vapor expansion and the formation of high-sulfidation gold deposits: Structural controls on hydrothermal alteration and ore mineralization

    Science.gov (United States)

    Berger, B.R.; Henley, R.W.

    2011-01-01

    High-sulfidation copper-gold lode deposits such as Chinkuashih, Taiwan, Lepanto, Philippines, and Goldfield, Nevada, formed within 1500. m of the paleosurface in volcanic terranes. All underwent an early stage of extensive advanced argillic silica-alunite alteration followed by an abrupt change to spatially much more restricted stages of fracture-controlled sulfide-sulfosalt mineral assemblages and gold-silver mineralization. The alteration as well as ore mineralization stages of these deposits were controlled by the dynamics and history of syn-hydrothermal faulting. At the Sulfate Stage, aggressive advanced argillic alteration and silicification were consequent on the in situ formation of acidic condensate from magmatic vapor as it expanded through secondary fracture networks alongside active faults. The reduction of permeability at this stage due to alteration decreased fluid flow to the surface, and progressively developed a barrier between magmatic-vapor expansion constrained by the active faults and peripheral hydrothermal activity dominated by hot-water flow. In conjunction with the increased rock strength resulting from alteration, subsequent fault-slip inversion in response to an increase in compressional stress generated new, highly permeable fractures localized by the embrittled, altered rock. The new fractures focused magmatic-vapor expansion with much lower heat loss so that condensation occurred. Sulfide Stage sulfosalt, sulfide, and gold-silver deposition then resulted from destabilization of vapor phase metal species due to vapor decompression through the new fracture array. The switch from sulfate to sulfide assemblages is, therefore, a logical consequence of changes in structural permeability due to the coupling of alteration and fracture dynamics rather than to changes in the chemistry of the fluid phase at its magmatic source. ?? 2010.

  8. Aquifer recharge from infiltration basins in a highly urbanized area: the river Po Plain (Italy)

    Science.gov (United States)

    Masetti, M.; Nghiem, S. V.; Sorichetta, A.; Stevenazzi, S.; Santi, E. S.; Pettinato, S.; Bonfanti, M.; Pedretti, D.

    2015-12-01

    Due to the extensive urbanization in the Po Plain in northern Italy, rivers need to be managed to alleviate flooding problems while maintaining an appropriate aquifer recharge under an increasing percentage of impermeable surfaces. During the PO PLain Experiment field campaign in July 2015 (POPLEX 2015), both active and under-construction infiltration basins have been surveyed and analyzed to identify appropriate satellite observations that can be integrated to ground based monitoring techniques. A key strategy is to have continuous data time series on water presence and level within the basin, for which ground based monitoring can be costly and difficult to be obtained consistently.One of the major and old infiltration basin in the central Po Plain has been considered as pilot area. The basin is active from 2003 with ground based monitoring available since 2009 and supporting the development of a calibrated unsaturated-saturated two-dimensional numerical model simulating the infiltration dynamics through the basin.A procedure to use satellite data to detect surface water change is under development based on satellite radar backscatter data with an appropriate incidence angle and polarization combination. An advantage of satellite radar is that it can observe surface water regardless of cloud cover, which can be persistent during rainy seasons. Then, the surface water change is correlated to the reservoir water stage to determine water storage in the basin together with integrated ground data and to give quantitative estimates of variations in the local water cycle.We evaluated the evolution of the infiltration rate, to obtain useful insights about the general recharge behavior of basins that can be used for informed design and maintenance. Results clearly show when the basin becomes progressively clogged by biofilms that can reduce the infiltration capacity of the basin by as much as 50 times compared to when it properly works under clean conditions.

  9. Determination of selected antibiotics in the Victoria Harbour and the Pearl River, South China using high-performance liquid chromatography-electrospray ionization tandem mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Xu Weihai [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, Guangzhou 510640 (China); School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510250 (China); Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong (China); Post-graduate School of the Chinese Academy of Sciences, Beijing 100039 (China); Zhang Gan [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, Guangzhou 510640 (China)]. E-mail: zhanggan@gig.ac.cn; Zou Shichun [School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510250 (China); Li Xiangdong [Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong (China); Liu Yuchun [School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510250 (China)

    2007-02-15

    Nine selected antibiotics in the Victoria Harbour of Hong Kong and the Pearl River at Guangzhou, South China, were analyzed using high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. The results showed that the concentrations of antibiotics were mainly below the limit of quantification (LOQ) in the marine water of Victoria Harbour. However, except for amoxicillin, all of the antibiotics were detected in the Pearl River during high and low water seasons with the median concentrations ranging from 11 to 67 ng/L, and from 66 to 460 ng/L, respectively; and the concentrations in early spring were about 2-15 times higher than that in summer with clearer diurnal variations. It was suggested that the concentrations of antibiotics in the high water season were more affected by wastewater production cycles due to quick refreshing rate, while those in the low water season may be more sensitive to the water column dynamics controlled by tidal processes in the river. - Antibiotics were found at high concentrations in an urban reach of Pearl River in southern China with contrast diurnal variations between the high and low water seasons.

  10. Exercise prevents weight gain and alters the gut microbiota in a mouse model of high fat diet-induced obesity.

    Directory of Open Access Journals (Sweden)

    Christian C Evans

    Full Text Available Diet-induced obesity (DIO is a significant health concern which has been linked to structural and functional changes in the gut microbiota. Exercise (Ex is effective in preventing obesity, but whether Ex alters the gut microbiota during development with high fat (HF feeding is unknown.Determine the effects of voluntary Ex on the gastrointestinal microbiota in LF-fed mice and in HF-DIO.Male C57BL/6 littermates (5 weeks were distributed equally into 4 groups: low fat (LF sedentary (Sed LF/Sed, LF/Ex, HF/Sed and HF/Ex. Mice were individually housed and LF/Ex and HF/Ex cages were equipped with a wheel and odometer to record Ex. Fecal samples were collected at baseline, 6 weeks and 12 weeks and used for bacterial DNA isolation. DNA was subjected both to quantitative PCR using primers specific to the 16S rRNA encoding genes for Bacteroidetes and Firmicutes and to sequencing for lower taxonomic identification using the Illumina MiSeq platform. Data were analyzed using a one or two-way ANOVA or Pearson correlation.HF diet resulted in significantly greater body weight and adiposity as well as decreased glucose tolerance that were prevented by voluntary Ex (p<0.05. Visualization of Unifrac distance data with principal coordinates analysis indicated clustering by both diet and Ex at week 12. Sequencing demonstrated Ex-induced changes in the percentage of major bacterial phyla at 12 weeks. A correlation between total Ex distance and the ΔCt Bacteroidetes: ΔCt Firmicutes ratio from qPCR demonstrated a significant inverse correlation (r2 = 0.35, p = 0.043.Ex induces a unique shift in the gut microbiota that is different from dietary effects. Microbiota changes may play a role in Ex prevention of HF-DIO.

  11. Exercise Prevents Weight Gain and Alters the Gut Microbiota in a Mouse Model of High Fat Diet-Induced Obesity

    Science.gov (United States)

    Evans, Christian C.; LePard, Kathy J.; Kwak, Jeff W.; Stancukas, Mary C.; Laskowski, Samantha; Dougherty, Joseph; Moulton, Laura; Glawe, Adam; Wang, Yunwei; Leone, Vanessa; Antonopoulos, Dionysios A.; Smith, Dan; Chang, Eugene B.; Ciancio, Mae J.

    2014-01-01

    Background Diet-induced obesity (DIO) is a significant health concern which has been linked to structural and functional changes in the gut microbiota. Exercise (Ex) is effective in preventing obesity, but whether Ex alters the gut microbiota during development with high fat (HF) feeding is unknown. Objective Determine the effects of voluntary Ex on the gastrointestinal microbiota in LF-fed mice and in HF-DIO. Methods Male C57BL/6 littermates (5 weeks) were distributed equally into 4 groups: low fat (LF) sedentary (Sed) LF/Sed, LF/Ex, HF/Sed and HF/Ex. Mice were individually housed and LF/Ex and HF/Ex cages were equipped with a wheel and odometer to record Ex. Fecal samples were collected at baseline, 6 weeks and 12 weeks and used for bacterial DNA isolation. DNA was subjected both to quantitative PCR using primers specific to the 16S rRNA encoding genes for Bacteroidetes and Firmicutes and to sequencing for lower taxonomic identification using the Illumina MiSeq platform. Data were analyzed using a one or two-way ANOVA or Pearson correlation. Results HF diet resulted in significantly greater body weight and adiposity as well as decreased glucose tolerance that were prevented by voluntary Ex (p<0.05). Visualization of Unifrac distance data with principal coordinates analysis indicated clustering by both diet and Ex at week 12. Sequencing demonstrated Ex-induced changes in the percentage of major bacterial phyla at 12 weeks. A correlation between total Ex distance and the ΔCt Bacteroidetes: ΔCt Firmicutes ratio from qPCR demonstrated a significant inverse correlation (r2 = 0.35, p = 0.043). Conclusion Ex induces a unique shift in the gut microbiota that is different from dietary effects. Microbiota changes may play a role in Ex prevention of HF-DIO. PMID:24670791

  12. The von Hippel-Lindau Chuvash mutation in mice alters cardiac substrate and high-energy phosphate metabolism.

    Science.gov (United States)

    Slingo, Mary; Cole, Mark; Carr, Carolyn; Curtis, Mary K; Dodd, Michael; Giles, Lucia; Heather, Lisa C; Tyler, Damian; Clarke, Kieran; Robbins, Peter A

    2016-09-01

    Hypoxia-inducible factor (HIF) appears to function as a global master regulator of cellular and systemic responses to hypoxia. HIF pathway manipulation is of therapeutic interest; however, global systemic upregulation of HIF may have as yet unknown effects on multiple processes. We used a mouse model of Chuvash polycythemia (CP), a rare genetic disorder that modestly increases expression of HIF target genes in normoxia, to understand what these effects might be within the heart. An integrated in and ex vivo approach was employed. Compared with wild-type controls, CP mice had evidence (using in vivo magnetic resonance imaging) of pulmonary hypertension, right ventricular hypertrophy, and increased left ventricular ejection fraction. Glycolytic flux (measured using [(3)H]glucose) in the isolated contracting perfused CP heart was 1.8-fold higher. Net lactate efflux was 1.5-fold higher. Furthermore, in vivo (13)C-magnetic resonance spectroscopy (MRS) of hyperpolarized [(13)C1]pyruvate revealed a twofold increase in real-time flux through lactate dehydrogenase in the CP hearts and a 1.6-fold increase through pyruvate dehydrogenase. (31)P-MRS of perfused CP hearts under increased workload (isoproterenol infusion) demonstrated increased depletion of phosphocreatine relative to ATP. Intriguingly, no changes in cardiac gene expression were detected. In summary, a modest systemic dysregulation of the HIF pathway resulted in clear alterations in cardiac metabolism and energetics. However, in contrast to studies generating high HIF levels within the heart, the CP mice showed neither the predicted changes in gene expression nor any degree of LV impairment. We conclude that the effects of manipulating HIF on the heart are dose dependent.

  13. Sex differences in high-fat diet-induced obesity, metabolic alterations and learning, and synaptic plasticity deficits in mice.

    Science.gov (United States)

    Hwang, Ling-Ling; Wang, Chien-Hua; Li, Tzu-Ling; Chang, Shih-Dar; Lin, Li-Chun; Chen, Ching-Ping; Chen, Chiung-Tong; Liang, Keng-Chen; Ho, Ing-Kang; Yang, Wei-Shiung; Chiou, Lih-Chu

    2010-03-01

    Obesity is a potential risk factor for cognitive deficits in the elder humans. Using a high-fat diet (HFD)-induced obese mouse model, we investigated the impacts of HFD on obesity, metabolic and stress hormones, learning performance, and hippocampal synaptic plasticity. Both male and female C57BL/6J mice fed with HFD (3 weeks to 9-12 months) gained significantly more weights than the sex-specific control groups. Compared with the obese female mice, the obese males had similar energy intake but developed more weight gains. The obese male mice developed hyperglycemia, hyperinsulinemia, hypercholesterolemia, and hyperleptinemia, but not hypertriglyceridemia. The obese females had less hyperinsulinemia and hypercholesterolemia than the obese males, and no hyperglycemia and hypertriglyceridemia. In the contextual fear conditioning and step-down passive avoidance tasks, the obese male, but not female, mice showed poorer learning performance than their normal counterparts. These learning deficits were not due to sensorimotor impairment as verified by the open-field and hot-plate tests. Although, basal synaptic transmission characteristics (input-output transfer and paired-pulse facilitation (PPF) ratio) were not significantly different between normal and HFD groups, the magnitudes of synaptic plasticity (long-term potentiation (LTP) and long-term depression (LTD)) were lower at the Schaffer collateral-CA1 synapses of the hippocampal slices isolated from the obese male, but not female, mice, as compared with their sex-specific controls. Our results suggest that male mice are more vulnerable than the females to the impacts of HFD on weight gains, metabolic alterations and deficits of learning, and hippocampal synaptic plasticity.

  14. Trigonelline attenuates hepatic complications and molecular alterations in high-fat high-fructose diet-induced insulin resistance in rats.

    Science.gov (United States)

    Afifi, Nehal A; Ramadan, Amer; Erian, Emad Y; Saleh, Dalia O; Sedik, Ahmed A; Badawi, Manal; El Hotaby, Walid

    2017-04-01

    The present study aimed to evaluate the effect of trigonelline (TRG) on the hepatic complications associated with high-fat high-fructose (HFHF) diet-induced insulin resistance (IR) in rats. IR was induced by giving a saturated fat diet and 10% fructose in drinking water to rats for 8 weeks. Insulin-resistant rats were orally treated with TRG (50 and 100 mg/kg), sitagliptin (SIT; 5 mg/kg), or a combination of TRG (50 mg/kg) and SIT (5 mg/kg) for 14 days. Liver homogenates were used for assessment of hepatic lipids, oxidative stress biomarkers, and inflammatory cytokines. Histopathological and DNA cytometry examinations were carried out for hepatic and pancreatic tissues. Hepatic tissues were examined using Fourier-transform infrared spectroscopy for assessment of any molecular changes. Results of the present study revealed that oral treatment of insulin-resistant rats with TRG or TRG in combination with SIT significantly decreased homeostatic model assessment of IR, hepatic lipids, oxidative stress biomarkers, and the inflammatory cytokines. TRG or TRG in combination with SIT ameliorated the histopathological, DNA cytometry, and molecular alterations induced by a HFHF diet. Finally, it can be concluded that TRG has beneficial effects on the hepatic complications associated with IR due to its hypoglycemic effect and antioxidant potential.

  15. Extracting dynamic topography from river profiles and cosmogenic nuclide geochronology in the Middle Atlas and the High Plateaus of Morocco

    Science.gov (United States)

    Pastor, Alvar; Babault, Julien; Owen, Lewis A.; Teixell, Antonio; Arboleya, María-Luisa

    2015-11-01

    The Moulouya river system has intensely eroded the Arhbalou, Missour, and Guercif Neogene foreland basins in northeastern Morocco, having changed from net aggradation during the Miocene-early Pliocene to net incision punctuated by alluvial fan deposition at late Pliocene or early Quaternary time. This region as a whole has experienced mantle-driven, surface uplift (dynamic topography) since the late Cenozoic, being locally affected by uplift due to crustal shortening and thickening of the Middle Atlas too. Knickpoints located along the major streams of the Moulouya fluvial network, appear on both the undeformed margins of the Missour and Guercif foreland basins (High Plateaus), as well as along the thrust mountain front of the southern Middle Atlas, where they reach heights of 800-1000 m. 500-550 m of the knickpoint vertical incision might be explained by long-wavelength mantle-driven dynamic surface uplift, whereas the remaining 450-500 m in the southern Middle Atlas front and 200-300 m in the northeastern Middle Atlas front seem to be thrust-related uplift of the Jebel Bou Naceur. Be-10 terrestrial cosmogenic nuclides have been used to date two Quaternary river terraces in the Chegg Ard valley at 62 ± 14 ka and 411 ± 55 ka. The dated terraces allow the incision rates associated with the frontal structures of the Middle Atlas to be estimated at ~ 0.3 mm yr- 1. Furthermore, these ages have served to evaluate mantle-driven regional surface uplift since the middle Pleistocene in the central Missour basin, yielding values of ~ 0.1-0.2 mm yr- 1.

  16. Higher high density lipoprotein cholesterol associated with moderate alcohol consumption is not related to altered plasma lecithin : cholesterol acyltransferase and lipid transfer protein activity levels

    NARCIS (Netherlands)

    Riemens, SC; vanTol, A; Hoogenberg, K; vanGent, T; Scheek, LM; Sluiter, WJ; Dullaart, RPF

    1997-01-01

    Lecithin:cholesterol acyltransferase (LCAT), cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) are important factors involved in HDL metabolism. Altered plasma activity levels of these factors could play a role in the increase in high density lipoprotein (HDL) choles

  17. Mice fed a high-fat diet supplemented with resistant starch display marked shifts in the liver metabolome concurrent with altered gut bacteria

    Science.gov (United States)

    High-amylose maize resistant starch type 2 (HAMRS2) is a fermentable dietary fiber known to alter the gut milieu, including the gut microbiota, which may explain reported effects of resistant starch to ameliorate obesity-associated metabolic dysfunction. Our working hypothesis is that HAMRS2-induced...

  18. Structural alterations of foreskin caused by chronic smoking may explain high levels of urethral reconstruction failure using foreskin flaps

    Directory of Open Access Journals (Sweden)

    João P. Rosado

    2012-08-01

    Full Text Available OBJECTIVES: The aim of the present study was to perform a stereological and biochemical analysis of the foreskin of smoker subjects. MATERIALS AND METHODS: Foreskin samples were obtained from 20 young adults (mean = 27.2 years old submitted to circumcision. Of the patients analyzed, one group (n = 10 had previous history of chronic smoking (a half pack to 3 packs per day for 3 to 13 years (mean = 5.8 ± 3.2. The control group included 10 nonsmoking patients. Masson's trichrome stain was used to quantify the foreskin vascular density. Weigert’s resorcin-fucsin stain was used to assess the elastic system fibers and Picrosirius red stain was applied to study the collagen. Stereological analysis was performed using the Image J software to determine the volumetric densities. For biochemical analysis, the total collagen was determined as µg of hydroxyproline per mg of dry tissue. Means were compared using the unpaired t-test (p < 0.05. RESULTS: Elastic system fibers of smokers was 42.5% higher than in the control group (p = 0.002. In contrast, smooth muscle fibers (p = 0.42 and vascular density (p = 0.16 did not show any significant variation. Qualitative analysis using Picrosirius red stain with polarized light evidenced the presence of type I and III collagen in the foreskin tissue, without significant difference between the groups. Total collagen concentration also did not differ significantly between smokers and non-smokers (73.1µg/mg ± 8.0 vs. 69.2µg/mg ± 5.9, respectively, p = 0.23. CONCLUSIONS: The foreskin tissue of smoking patients had a significant increase of elastic system fibers. Elastic fibers play an important role in this tissue’s turnover and this high concentration in smokers possibly causes high extensibility of the foreskin. The structural alterations in smokers’ foreskins could possibly explain the poor results in smoking patients submitted to foreskin fasciocutaneous flaps in urethral reconstruction surgery.

  19. Identification of Unknown Microcontaminants in Dutch River Water by Liquid Chromatography-High Resolution Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy.

    NARCIS (Netherlands)

    Leerdam, van J.A.; Vervoort, J.J.M.; Stroomberg, G.; Voogt, de P.

    2014-01-01

    In the past decade during automated surface water monitoring in the river Meuse at border station Eijsden in The Netherlands, a set of unknown compounds were repeatedly detected by online liquid chromatography-diode-array detection in a relatively high signal intensity. Because of the unknown nature

  20. Identification of unknown microcontaminants in Dutch river water by liquid chromatography-high resolution mass spectrometry and nuclear magnetic Resonance spectroscopy

    NARCIS (Netherlands)

    van Leerdam, J.A.; Vervoort, J.; Stroomberg, G.; de Voogt, P.

    2014-01-01

    In the past decade during automated surface water monitoring in the river Meuse at border station Eijsden in The Netherlands, a set of unknown compounds were repeatedly detected by online liquid chromatography-diode-array detection in a relatively high signal intensity. Because of the unknown nature

  1. Modelling high-resolution snow cover precipitation supply for German river catchments with SNOW 4

    Science.gov (United States)

    Böhm, Uwe; Reich, Thomas; Schneider, Gerold; Fiedler, Anett

    2013-04-01

    Formation of snow cover causes a delayed response of surface to precipitation. Both melting of snow and release of liquid water retained within the snow cover form precipitation supply which contributes to runoff and infiltration. The model SNOW 4 is developed to simulate snow cover accumulation and depletion and the resulting precipitation supply on a regular grid. The core of the model is formed by a set of equations which describe the snow cover energy and mass balance. The snow surface energy balance is calculated as a result of the radiation balance and the heat fluxes between atmosphere, soil and snow cover. The available melting heat enters the mass balance computation part of the model and melting of snow or freezing of liquid water within the snow layer takes place depending on its sign. Retention, aging and snow cover regeneration are taken into consideration. The model runs operationally 4 times a day and provides both a snow cover and precipitation supply analysis for the last 30 hours and a forecast for up to 72 hours. For the 30-hour analysis, regionalised observations are used both to define the initial state and force the model. Hourly measurements of air temperature, water vapour pressure, wind speed, global radiation or sunshine duration and precipitation are interpolated to the model grid. For the forecast period, SNOW 4 obtains the required input data from the operational products of the COSMO-EU weather forecast model. The size of a grid box is 1km2. The model area covers a region of 1100x1000km2 and includes the catchments of the German rivers completely. The internal time step is set to 1 hour. Once a day, the compliance between model and regionalized snow cover data is assessed. If discrepancies exceed certain thresholds, the model must be adjusted by a weighted approach towards the observations. The model simulations are updated every six hours based on the most recent observations and weather forecasts. The model works operationally since

  2. High Resolution Topography and Multiple Seismic Uplift on the Main Frontal Thrust near the Ratu River, Eastern Nepal

    Science.gov (United States)

    Karakas, C.; Tapponnier, P.; Sapkota, S. N.; Coudurier Curveur, A.; Ildefonso, S.; Gao, M.; Bollinger, L.; Klinger, Y.; Tiwari, D. R.

    2015-12-01

    New data at several more sites along the Main Frontal Thrust between 85°49' to 86°27' E corroborates the surface emergence of the great M ≈ 8.4, 1934 Bihar-Nepal and 1255 AD earthquakes. Here we show unambiguous evidences of surface rupture and co-seismic uplift in the Ratu river area based on high-resolution geomorphic surveys. We present a refined map of uplifted terrace surfaces and abandoned paleo-channels truncated by the MFT, obtained by newly acquired high resolution Digital Elevation Models from Total station, Terrestrial Lidar Scanner (TLS), Unmanned Aerial Vehicle (UAV) and kinematic GPS surveys. In the Ratu valley, using these new high resolution topographic datasets, we identify five and possibly seven distinct terrace levels uplifted parallel to the riverbed, that cap the folded Siwaliks. Such measurements may be taken to imply broadly characteristic increments of throw during sequences of at least five to seven events of riverbed abandonment related to co-seismic uplifts. Newly collected detrital charcoals from several pits and from a rejuvenated paleoseismological wall will help assess more precisely uplift and shortening rates over the length of segments of the MFT east and west of Bardibas. A regional comparison of comparable long term paleoseismological data at other sites along the 1934 rupture length is in progress.

  3. High-resolution sedimentary record of hydrocarbon contaminants in a core from the major reaches of the Pearl River, China

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The concentrations and compositions of hydrocarbon contaminants, and molecularmarker indices in modern sediments from a core in the major reaches of the Pearl River were investigated. The sedimentary record of hydrocarbons in the core, in combination with 210pb-dating,was used to reconstruct the pollution history of hydrocarbon pollutants in the Pearl River in the past 100 years.

  4. Occurrence of polycyclic aromatic hydrocarbons in surface sediments of a highly urbanized river system with special reference to energy consumption patterns

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, K.; Wang, J.Z.; Liang, B.; Zeng, E.Y. [Chinese Academy Science, Guangzhou (China)

    2011-06-15

    Sediment samples collected from downstream of the Dongjiang River, a highly urbanized river network within the Pearl River Delta of South China, were analyzed for 28 polycyclic aromatic hydrocarbons (PAHs). Total concentrations of 28 PAHs, 16 priority PAHs designated by the United States Environmental Protection Agency (USEPA) and the seven carcinogenic PAHs classified by the USEPA ranged from 480 to 4600, 100 to 3400 and 10 to 1700 ng/g dry weight, respectively. Principal component analysis-based stepwise multivariate linear regression showed that sediment PAHs were predominantly derived from coal combustion, refined fossil fuel combustion and oil spills, accounting for 37%, 32% and 23%, respectively, of the total loading. The levels of sediment PAHs remained steady from 2002 to 2008, during which fossil fuel consumption had doubled, probably reflecting efforts to control PAH emissions from fossil fuel combustion. Finally, use of natural gas and liquefied petroleum gas in automobiles should be encouraged to improve environmental quality.

  5. High burden of copy number alterations and c-MYC amplification in prostate cancer from BRCA2 germline mutation carriers

    NARCIS (Netherlands)

    Castro, E.; Jugurnauth-Little, S.; Karlsson, Q.; Al-Shahrour, F.; Pineiro-Yanez, E.; Van de Poll, F.; Leongamornlert, D.; Dadaev, T.; Govindasami, K.; Guy, M.; Eeles, R.; Kote-Jarai, Z.; Oosterwijk, J. C.

    2015-01-01

    Background: Germline BRCA2 mutations are associated with poorer outcome prostate cancer ( PCa) compared with sporadic tumours but this association remains to be characterised. In this study, we aim to assess if there is a signature set of copy number alterations ( CNA) that could aid to the identifi

  6. Biodegradation of high concentrations of mixed polycyclic aromatic hydrocarbons by indigenous bacteria from a river sediment: a microcosm study and bacterial community analysis.

    Science.gov (United States)

    Muangchinda, Chanokporn; Yamazoe, Atsushi; Polrit, Duangporn; Thoetkiattikul, Honglada; Mhuantong, Wuttichai; Champreda, Verawat; Pinyakong, Onruthai

    2017-02-01

    This study assessed the biodegradation of mixtures of polycyclic aromatic hydrocarbons (PAHs) by indigenous bacteria in river sediment. Microcosms were constructed from sediment from the Chao Phraya River (the main river in Thailand) by supplementation with high concentrations of fluorene, phenanthrene, pyrene (300 mg kg(-1) of each PAH), and acenaphthene (600 mg kg(-1)). Fluorene and phenanthrene were completely degraded, whereas 50% of the pyrene and acenaphthene were removed at the end of the incubation period (70 days). Community analyses revealed the dynamics of the bacterial profiles in the PAH-degrading microcosms after PAH exposure. Actinobacteria predominated and became significantly more abundant in the microcosms after 14 days of incubation at room temperature under aerobic conditions. Furthermore, the remaining PAHs and alpha diversity were positively correlated. The sequencing of clone libraries of the PAH-RHDα genes also revealed that the dioxygenase genes of Mycobacterium sp. comprised 100% of the PAH-RHDα library at the end of the microcosm setup. Moreover, two PAH-degrading Actinobacteria (Arthrobacter sp. and Rhodococcus ruber) were isolated from the original sediment sample and showed high activity in the degradation of phenanthrene and fluorene in liquid cultivation. This study reveals that indigenous bacteria had the ability to degrade high concentrations of mixed PAHs and provide clear evidence that Actinobacteria may be potential candidates to play a major role in PAH degradation in the river sediment.

  7. High-resolution insights into episodes of crystallization, hydrothermal alteration and remelting in the Skaergaard intrusive complex

    Science.gov (United States)

    Wotzlaw, Jörn-Frederik; Bindeman, Ilya N.; Schaltegger, Urs; Brooks, C. Kent; Naslund, H. Richard

    2012-11-01

    This paper presents a new high-precision zircon U-Pb geochronological view on the crystallization and assembly process of one of the most important and intensely studied intrusive bodies on Earth—the Skaergaard intrusion in East Greenland. With analytical uncertainties of a few tens of thousands of years, we were able to resolve several important events during cooling of this intrusion. Initial cooling of the shallowly intruded ˜300 km3 of tholeiitic basaltic magma from liquidus to zircon saturation at ˜1000 °C is recorded by a precise zircon crystallization age of 55.960±0.018 Ma of an intercumulus gabbroic pegmatite in the lower portion of the intrusion. Based on this zircon crystallization age and a published cooling model we estimate the "true" age of emplacement to be ˜56.02 Ma. The last portions of Skaergaard appear to crystallize completely ˜100 ka after emplacement as recorded by abundant ˜55.91-55.93 Ma zircons in the Sandwich Horizon (SH), where lower and upper solidification fronts met. Intrusion of an isotopically distinct new magma batch, the ˜600 m thick Basistoppen Sill, into the solidified upper portion of Skaergaard, happened at 55.895±0.018 Ma, suggesting close timing between crystallization of evolved rocks around the SH and intrusion of the Basistoppen Sill. The novel result of this work is the demonstration that zircons in the SH, >100 m below the Basistoppen contact, have a bimodal age distribution, with the youngest population of 55.838±0.019 Ma postdating intrusion of the Basistoppen Sill by 57±37 ka. Oxygen isotope analyses reveal that SH zircons are low and heterogeneous with respect to δ18O. These results support the proposed conclusion that the SH crystallized twice: it was fully crystalline, then hydrothermally-altered by low-δ18O surface waters and subsequently partially remelted, triggered by heat of the Basistoppen Sill. The low-degree partial melt generated during remelting partially migrated upward by intergranular

  8. CHARACTERISTICS AND CAUSE ANALYSIS OF WESTERN PACIFIC SUBTROPICAL HIGH DURING THE HUAIHE RIVER FLOODS IN 2003

    Institute of Scientific and Technical Information of China (English)

    JIN Rong-hua; Jiao Mei-yan; Xu Jing; Qin Hua-feng

    2006-01-01

    @@ 1 INTRODUCTION In summer, different assembly of the intensity,location and vertical structure of the subtropical high and the earlier/later time of its seasonal northwards jump bring about different precipitation patterns over China. Therefore, subtropical high activity and its cause during the occurrence of extreme climatic event over China and the cause of China drought/flood are studied to improve weather forecasting.

  9. High Recharge Areas in the Choushui River Alluvial Fan (Taiwan Assessed from Recharge Potential Analysis and Average Storage Variation Indexes

    Directory of Open Access Journals (Sweden)

    Jui-Pin Tsai

    2015-03-01

    Full Text Available High recharge areas significantly influence the groundwater quality and quantity in regional groundwater systems. Many studies have applied recharge potential analysis (RPA to estimate groundwater recharge potential (GRP and have delineated high recharge areas based on the estimated GRP. However, most of these studies define the RPA parameters with supposition, and this represents a major source of uncertainty for applying RPA. To objectively define the RPA parameter values without supposition, this study proposes a systematic method based on the theory of parameter identification. A surrogate variable, namely the average storage variation (ASV index, is developed to calibrate the RPA parameters, because of the lack of direct GRP observations. The study results show that the correlations between the ASV indexes and computed GRP values improved from 0.67 before calibration to 0.85 after calibration, thus indicating that the calibrated RPA parameters represent the recharge characteristics of the study area well; these data also highlight how defining the RPA parameters with ASV indexes can help to improve the accuracy. The calibrated RPA parameters were used to estimate the GRP distribution of the study area, and the GRP values were graded into five levels. High and excellent level areas are defined as high recharge areas, which composed 7.92% of the study area. Overall, this study demonstrates that the developed approach can objectively define the RPA parameters and high recharge areas of the Choushui River alluvial fan, and the results should serve as valuable references for the Taiwanese government in their efforts to conserve the groundwater quality and quantity of the study area.

  10. Geochemical characteristics and organic carbon sources within the upper reaches of the Xi River, southwest China during high flow

    Indian Academy of Sciences (India)

    Junyu Zou

    2017-02-01

    Carbon (POC, DOC) and carbon isotopes (δ13C) within two headwater tributaries to the Xi River Basin, southwest China were analyzed to document the geochemical characteristics and sources of organic carbon (C) within basins characterized by a monsoonal climate and karst landforms. δ¹³CPOC value and C/N ratio data indicate that suspended soil organic carbon (SOC) was an important source of POC in both the Nanpan and Beipan rivers (i.e., the studied tributaries). However, differences in C sources exist between the Nanpan and Beipan River Basins. Higher terrestrial plants supplied a portion of the POC within the Beipan River. In contrast, the Nanpan River was characterized by an inverse correlation between POC and DOC, and a positive relationship between the δ¹³C values. These trends indicate that DOC within the Nanpan River was partly derived from the degradation of soil C within the water column. In addition, the interception of C by hydrological projects (e.g., dams) positioned along the Nanpan River led to higher DOC/POC ratios. In contrast, within the Beipan River δ¹³CDOC values range from −20 to −25.2‰ and are consistent with ratios associated with soil C, suggesting that leaching of C from catchment soil was the dominant source of DOC. Organic C in tributaries to the Beipan River may also have been derived from intense upland soil erosion, a process that resulted in the lowest DOC/POC ratios. The collected data indicate that land-use changes have potentially influenced regionalto local-scale organic C budgets within subtropical basins subjected to karstification.

  11. Geochemical characteristics and organic carbon sources within the upper reaches of the Xi River, southwest China during high flow

    Science.gov (United States)

    Zou, Junyu

    2017-02-01

    Carbon (POC, DOC) and carbon isotopes (δ13C) within two headwater tributaries to the Xi River Basin, southwest China were analyzed to document the geochemical characteristics and sources of organic carbon (C) within basins characterized by a monsoonal climate and karst landforms. δ13 C POC value and C/N ratio data indicate that suspended soil organic carbon (SOC) was an important source of POC in both the Nanpan and Beipan rivers (i.e., the studied tributaries). However, differences in C sources exist between the Nanpan and Beipan River Basins. Higher terrestrial plants supplied a portion of the POC within the Beipan River. In contrast, the Nanpan River was characterized by an inverse correlation between POC and DOC, and a positive relationship between the δ13C values. These trends indicate that DOC within the Nanpan River was partly derived from the degradation of soil C within the water column. In addition, the interception of C by hydrological projects (e.g., dams) positioned along the Nanpan River led to higher DOC/POC ratios. In contrast, within the Beipan River δ13C DOC values range from -20 to -25.2 ‰ and are consistent with ratios associated with soil C, suggesting that leaching of C from catchment soil was the dominant source of DOC. Organic C in tributaries to the Beipan River may also have been derived from intense upland soil erosion, a process that resulted in the lowest DOC/POC ratios. The collected data indicate that land-use changes have potentially influenced regional- to local-scale organic C budgets within subtropical basins subjected to karstification.

  12. Hydrogeological and hydrochemical characterization of unconfined aquifer located in the alteration mantle of the Serra Geral Formation, in the Taquari-Antas river basin, northeast of Rio Grande do Sul State

    Directory of Open Access Journals (Sweden)

    Gisele Cemin

    2012-08-01

    Full Text Available This paper presents the hydrogeological and hydrochemical characterization of the unconfined aquifer located in the alteration mantle in the volcanic rocks from Serra Geral Formation, in the northeast region of Rio Grande do Sul State. This aquifer is conditioned by the soil, topography, lithology and climate, being characterized by a saturated layer thinner than 1,7 m, static levels between 0 and 1 m and low flow (<0,5 m3/h. These waters are of calcium or magnesium bicarbonate type, and low alkalinity and electrical conductivity, low calcium, magnesium, sodium, potassium and chloride concentrations, and high iron and manganese concentrations. Additionally, these waters have high concentration of nitrates (mean above 10 mg/L and the presence of total coliforms. These characteristics evidence a quick circulation between the recharging and discharge zones in the aquifer.

  13. Alteration of Fractured Rocks Due to Coupled Chemical and Mechanical Processes: High-Resolution Simulations and Experimental Observations

    Science.gov (United States)

    Ameli, Pasha

    Engineering activities such as enhanced geothermal energy production and improved oil recovery techniques are heavily dependent on the permeability of the subsurface, while others such as CO2 sequestration and nuclear waste disposal rely on the efficiency of rock formations as transport barriers. In either case fractures provide the main pathways for fluid flow and transport, especially in rocks with lower matrix porosity. Laboratory experiments aimed at quantifying the chemo-mechanical responses of fractures have shown a range of results, some of which contradict simple conceptual models. For example, under conditions favoring mineral dissolution, where one would expect an overall increase in permeability, experiments show that permeability increases under some conditions and decreases under others. Recent experiments have attempted to link these core-scale observations to the relevant small-scale processes occurring within fractures. Results suggest that the loss of mechanical strength in asperities due to chemical alteration may cause non-uniform deformation and alteration of fracture apertures. However, due to the lack of direct micro-scale measurements of the coupled chemical and mechanical processes that lead to alteration of contacting fracture surfaces, our ability to predict the long-term evolution of fractures is still limited. To explore the processes that control permeability evolution, I developed a computational model that uses micro-scale surface roughness and explicitly couples dissolution and elastic deformation to calculate local alterations in fracture aperture under chemical and mechanical stresses. A depth-averaged algorithm of fracture flow is used to model reactive transport and chemical alteration of the fracture surfaces. Then, I deform the resulting altered fracture-surfaces using an algorithm that calculates the elastic deformation. The results of the model are compared with flow-through experiments conducted on fractured limestone. The

  14. Occurrence of polycyclic aromatic hydrocarbons in surface sediments of a highly urbanized river system with special reference to energy consumption patterns

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Kai [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); Graduate School of Chinese Academy of Sciences, Beijing 100049 (China); Wang Jizhong [CAS Key Laboratory of Crust-Mantle Materials and Environment, School of Earth and Space Science, University of Science and Technology of China, Hefei 230026 (China); Liang Bo [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); Graduate School of Chinese Academy of Sciences, Beijing 100049 (China); Zeng, Eddy Y., E-mail: eddyzeng@gig.ac.cn [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China)

    2011-06-15

    Sediment samples collected from downstream of the Dongjiang River, a highly urbanized river network within the Pearl River Delta of South China, were analyzed for 28 polycyclic aromatic hydrocarbons (PAHs). Total concentrations of 28 PAHs, 16 priority PAHs designated by the United States Environmental Protection Agency (USEPA) and the seven carcinogenic PAHs classified by the USEPA ranged from 480 to 4600, 100 to 3400 and 10 to 1700 ng/g dry weight, respectively. Principal component analysis-based stepwise multivariate linear regression showed that sediment PAHs were predominantly derived from coal combustion, refined fossil fuel combustion and oil spills, accounting for 37%, 32% and 23%, respectively, of the total loading. The levels of sediment PAHs remained steady from 2002 to 2008, during which fossil fuel consumption had doubled, probably reflecting efforts to control PAH emissions from fossil fuel combustion. Finally, use of natural gas and liquefied petroleum gas in automobiles should be encouraged to improve environmental quality. - Highlights: > PAHs in sediment showed similar input sources as those in air particles. > Combustion of fossil fuel is the predominant source for sediment PAHs. > Local efforts to control PAHs emissions from fossil fuel combustion were effective. > Promotion of natural gas and liquefied petroleum gas can reduce emissions of PAHs. - Occurrence of PAHs in surface sediments from a highly urbanized river system is assessed in relation to energy consumption patterns.

  15. Ginseng Extracts Restore High-Glucose Induced Vascular Dysfunctions by Altering Triglyceride Metabolism and Downregulation of Atherosclerosis-Related Genes

    Directory of Open Access Journals (Sweden)

    Gabriel Hoi-huen Chan

    2013-01-01

    Full Text Available The king of herbs, Panax ginseng, has been used widely as a therapeutic agent vis-à-vis its active pharmacological and physiological effects. Based on Chinese pharmacopeia Ben Cao Gang Mu and various pieces of literature, Panax ginseng was believed to exert active vascular protective effects through its antiobesity and anti-inflammation properties. We investigated the vascular protective effects of ginseng by administrating ginseng extracts to rats after the induction of diabetes. We found that Panax ginseng can restore diabetes-induced impaired vasorelaxation and can reduce serum triglyceride but not cholesterol level in the diabetic rats. The ginseng extracts also suppressed the expression of atherosclerosis-related genes and altered the expression of lipid-related genes. The results provide evidence that Panax ginseng improves vascular dysfunction induced by diabetes and the protective effects may possibly be due to the downregulation of atherosclerosis-related genes and altered lipid metabolism, which help to restore normal endothelium functions.

  16. Dissolved Organic Matter in the Yukon River Basin

    Science.gov (United States)

    Aiken, G.; Striegl, R.; Schuster, P.

    2004-12-01

    Source materials, watershed geochemistry, oxidative processes and hydrology exert strong influences on the nature and reactivity of dissolved organic matter (DOM) in aquatic systems. At present, a critical question in carbon cycling is how climate change could alter the fate and chemical nature of dissolved organic carbon (DOC) released from watersheds, particularly those underlain by permafrost, and transported to rivers, lakes, estuaries and coastal waters. The spatial and temporal variability of DOM in surface waters associated with the Yukon River are being studied to better define processes controlling DOM in this system. Like many northern ecosystems, the Yukon River Basin is experiencing melting permafrost, drying of upland soils and changing wetland environments. Study results indicate that the transport of DOM in the river and its major tributaries is strongly seasonally dependent. Specific ultraviolet absorbance (SUVA) data, an excellent indicator of aromatic carbon content of DOM, also indicate a large variation in the chemical nature of the organic matter transported by the river. Lowest DOC concentrations and SUVA values were observed for samples collected in the winter under low flow conditions and for tributaries dominated by ground water inputs. Greatest DOC concentrations and SUVA values were measured on samples collected during the spring on the leading part of the hydrograph. High SUVA values are indicative of greater amounts of organic material originating from higher plants that are present in upper soil horizons and wetlands of the watershed. Aquatic humic substances collected from the Yukon River during the snowmelt period were found to have low nitrogen contents and greater amounts of aromatic C relative to samples from other aquatic environments. Low N content and high aromaticity are indicative of humic substances evolved from higher plant sources with little alteration resulting from microbial degradation or soil interactions. In addition

  17. Linking carbon and iron cycles by investigating transport, fate and mineralogy of iron-bearing colloids from peat-draining rivers - Scotland as model for high-latitude rivers

    Science.gov (United States)

    Wood, Deborah; Crocket, Kirsty; Brand, Tim; Stutter, Marc; Wilson, Clare; Schröder, Christian

    2016-04-01

    Linking carbon and iron cycles by investigating transport, fate and mineralogy of iron-bearing colloids from peat-draining rivers - Scotland as model for high-latitude rivers Wood, D.A¹, Crocket, K², Brand, T², Stutter, M³, Wilson, C¹ & Schröder, C¹ ¹Biological and Environmental Sciences, University of Stirling, Stirling, FK9 4LA ²Scottish Association for Marine Science, University of the Highlands and Islands, Dunbeg, Oban, PA37 1QA ³James Hutton Institute, Craigiebuckler, Aberdeen, AB15 8QH The biogeochemical iron cycle exerts significant control on the carbon cycle¹. Iron is a limiting nutrient in large areas of the world's oceans and its bioavailability controls CO2 uptake by marine photosynthesizing microorganisms. While atmospheric iron inputs to the open ocean have been extensively measured, global river inputs have likely been underestimated because most major world rivers exhibit extensive iron removal by flocculation and sedimentation during seawater mixing. Iron minerals and organic matter mutually stabilise each other², which results in a 'rusty carbon sink' in sediments³ on the one hand but may also enhance transport beyond the salinity gradient on the other. Humic-rich, high latitude rivers have a higher iron-carrying capacity⁴-⁶ but are underrepresented in iron flux calculations. The West Coast sea lochs in Scotland are fed by predominantly peatland drainage catchments, and the rivers entering the sea lochs carry a high load of organic matter. The short distance between many of these catchments and the coastal ocean facilitates source-to-sea research investigating transport, fate and mineralogy of iron-bearing colloids providing a good analogue for similar high latitude fjordic systems. We use SeaFAST+ICP-MS and Mössbauer spectroscopy to survey trace metal concentrations, with emphasis on iron concentrations, speciation and mineralogy, across salinity gradients. In combination with ultra-filtration techniques, this allows

  18. Aragonite, breunnerite, calcite and dolomite in the CM carbonaceous chondrites: High fidelity recorders of progressive parent body aqueous alteration

    OpenAIRE

    Lee, Martin R.; Lindgren, Paula; Sofe, Mahmood R.

    2014-01-01

    Carbonate minerals in CM carbonaceous chondrite meteorites, along with the silicates and sulphides with which they are intergrown, provide a detailed record of the nature and evolution of parent body porosity and permeability, and the chemical composition, temperature and longevity of aqueous solutions. Fourteen meteorites were studied that range in petrologic subtype from mildly aqueously altered CM2.5 to completely hydrated CM2.0. All of them contain calcite, whereas aragonite occurs only i...

  19. Altered expression of urokinase-type plasminogen activator and plasminogen activator inhibitor in high-risk soft tissue sarcomas

    OpenAIRE

    Benassi, M S; Ponticelli, F.; Azzoni, E.; Gamberi, G.; Pazzaglia, L.; Chiechi, A.; Conti, A; Spessotto, P.; Scapolan, M; Pignotti, E; P Bacchini; Picci, P.

    2007-01-01

    In recent years, classification of soft-tissue sarcomas (STS) has improved with cytogenetic analyses, but their clinical behavior is still not easily predictable. The aim of this study was to detect alterations in the urokinase-type plasminogen system, involved in tumor growth and invasion, by comparing mRNA levels of its components with those of paired normal tissues, and relating them with patient clinical course. Real-time PCR was performed on human STS cell lines a...

  20. Hanford Site River Protection Project High-Level Waste Safe Storage and Retrieval

    Energy Technology Data Exchange (ETDEWEB)

    Aromi, E. S.; Raymond, R. E.; Allen, D. I.; Payne, M. A.; DeFigh-Price, C.; Kristofzski, J. G.; Wiegman, S. A.

    2002-02-25

    This paper provides an update from last year and describes project successes and issues associated with the management and work required to safely store, enhance readiness for waste feed delivery, and prepare for treated waste receipts for the approximately 53 million gallons of mixed and high-level waste currently in aging tanks at the Hanford Site. The Hanford Site is a 560 square-mile area in southeastern Washington State near Richland, Washington.

  1. Antimicrobial activity of filamentous fungi isolated from highly antibiotic-contaminated river sediment

    OpenAIRE

    2012-01-01

    Background: Filamentous fungi are well known for their production of substances with antimicrobial activities, several of which have formed the basis for the development of new clinically important antimicrobial agents. Recently, environments polluted with extraordinarily high levels of antibiotics have been documented, leading to strong selection pressure on local sentinel bacterial communities. In such microbial ecosystems, where multidrug-resistant bacteria are likely to thrive, it is poss...

  2. River recharge sources and the partitioning of catchment evapotranspiration fluxes as revealed by stable isotope signals in a typical high-elevation arid catchment

    Science.gov (United States)

    Guo, Xiaoyu; Tian, Lide; Wang, Lei; Yu, Wusheng; Qu, Dongmei

    2017-06-01

    Catchment-scale hydrological cycles are expected to suffer more extremes under a background of climate change. Quantifying hydrological changes in high and remote areas is practically challenging. However, stable isotopes in river water can be seen to vary, dependent upon the combined influence exerted by recharge sources and local climatic conditions; the study of river water stable isotopes can therefore provide a meaningful method for delineating catchment-scale hydrological studies. In this study, we present high-resolution time series of river δ18O and d-excess values; additionally, we identify the seasonal dynamics of river recharge sources and major components of the catchment-scale water balance, together with precipitation and groundwater isotopes, and concurrent meteorological data recorded in Magazangbu catchment on the northwestern Tibetan Plateau (TP). Using isotopic analysis, and within a proportional framework, we partitioned the isotopic fractionation (E1) or non-fractionation (E2) from soil evaporation fluxes (Esoil) apparent in different processes, using NDVI (Normal Differential Vegetation Index) data collected by MODIS satellites to calculate the vegetation fractional coverage (VFC), and Global Land Data Assimilation System (GLDAS) records to determine evapotranspiration data (ET). Finally, the contributions made by each ET component (Esoil and plant transpiration) to total catchment ET were computed for the high and remote northwestern TP. Our results show that: (1) river δ18O values were high in summer and low in winter, while d-excess values displayed a contrary seasonal cycle; (2) for the monsoon period, precipitation contributed 60.6% to Magazangbu catchment runoff. Deeper groundwater was the main water source for the winter low base flow, and shallow groundwater or high elevation snowmelt was the principal component of the spring thaw and autumn freezing periods; and (3) a substantial proportion of Esoil (96.4% annually; 92.2% during

  3. Mercury exports from a High-Arctic river basin in Northeast Greenland (74°N) largely controlled by glacial lake outburst floods.

    Science.gov (United States)

    Søndergaard, Jens; Tamstorf, Mikkel; Elberling, Bo; Larsen, Martin M; Mylius, Maria Rask; Lund, Magnus; Abermann, Jakob; Rigét, Frank

    2015-05-01

    Riverine mercury (Hg) export dynamics from the Zackenberg River Basin (ZRB) in Northeast Greenland were studied for the period 2009-2013. Dissolved and sediment-bound Hg was measured regularly in the Zackenberg River throughout the periods with running water (June-October) and coupled to water discharge measurements. Also, a few samples of snow, soil, and permafrost were analysed for Hg. Mean concentrations of dissolved and sediment-bound Hg in the river water (±SD) were 0.39 ± 0.13 and 5.5 ± 1.4 ngL(-1), respectively, and mean concentrations of Hg in the river sediment were 0.033 ± 0.025 mg kg(-1). Temporal variations in river Hg were mainly associated with snowmelt, sudden erosion events, and outburst floods from a glacier-dammed lake in the upper part of the ZRB. Annual Hg exports from the 514 km(2) ZRB varied from 0.71 to >1.57 kg and the majority (86-96%) was associated with sediment-bound Hg. Hg yields from the ZRB varied from 1.4-3.1 gH gk m(-2)yr(-1) and were among the highest yields reported from Arctic river basins. River exports of Hg from ZRB were found to be largely controlled by the frequency, magnitude and timing of the glacial lake outburst floods, which occurred in four of the five years in July-August. Floods accounted for 5 to >10% of the annual water discharge, and up to >31% of the annual Hg export. Also, the winter snowfall and the summer temperatures were found to be important indirect controls on the annual Hg export. The occurrence and timing of glacial lake outburst floods in the ZRB in late summer at the time of maximum soil thaw depth, the location of the glacier in the upper ZRB, and increased thawing of the permafrost in Zackenberg in recent years leading to destabilisation of river banks are considered central factors explaining the high fraction of flood-controlled Hg export in this area.

  4. Tectonic, climatic, and anthropogenic influences on ultra-high rate of bedrock incision in the Ta-An River Gorge, western Taiwan

    Science.gov (United States)

    Chen, M.; Lee, J.; Hsieh, M.; Chan, Y.; Frankel, K.

    2008-12-01

    We characterize geomorphic features of a bedrock gorge with a 12-m-high knickpoint formed in the lower Ta- An River near Diaoshenshan, Taiwan that has experienced 20 to 30 m of incision since 1999. Although the same spot was uplifted 10 m during the 1999 Mw = 7.6 Chi-Chi earthquake, this amount of coseismic displacement does not fully explain the overdeepening of the channel longitudinal profile. This study focuses on a ~ 4 km reach of the lower Ta-An River, which is underlain by interbedded sandstone and shale, with the pop-up structure in the uppermost 1 km. A 700-m-long, > 20-m-deep bedrock gorge is located along this reach. River discharge and geomorphic data including knickpoint location, channel slope, depth of gorge, and height of hanging valleys are utilized to derive rates, patterns, and evolution of the bedrock incision. Two stages of fluvial incision are identified in the gorge zone: (1) 3 to 5 m of total incision from 1999 to 2003, and (2) 15 to 20 m of total incision since 2004. The knickpoint is migrating upstream at a rate of approximately 100 m/yr, which has elongated the extent of the gorge from the west limit of the pop-up structure since 2004. Significant amounts of knickpoint retreat occur during the annual summer typhoon season. We interpret that the Ta-An River responded to the 1999 coseismic uplift in the first stage of incision in similar fashion to the Ta-Chia River, located 5 km to the south. The accelerated bedrock incision in the second stage, which deepened the bedrock gorge since 2004, is correlated with human-induced sediment supply change and large flooding events. Heavy sediment mining over the study area since the post- earthquake period and closure of the Shihlin Dam 15 km upstream significantly decreased sediment supply to the channel. Moreover, both yearly precipitation and river discharge from 2003 to the present are significantly above average for the past 105 and 32 years. In 2005, the 33 year maximum daily discharge of 3679

  5. Contested Rivers

    DEFF Research Database (Denmark)

    Gorm Hansen, Louise Lyngfeldt

    explores translocal connections through ethnographic fieldwork at a global water conference and preliminary fieldwork at chosen locations on China's Nu River. The Nu River is one of the last undammed rivers in Asia and runs through China close to the Chinese-Burmese border, then flows into the Andaman Sea...

  6. The Impact of Indian Ocean Variability on HighTemperature Extremes across the Southern Yangtze River Valley in Late Summer

    Institute of Scientific and Technical Information of China (English)

    HU Kaiming; HUANG Gang; QU Xia; HUANG Ronghui

    2012-01-01

    In this study,the teleconnection between Indian Ocean sea surface temperature anomalies (SSTAs) and the frequency of high temperature extremes (HTEs) across the southern Yangtze River valley (YRV) was investigated.The results indicate that the frequency of HTEs across the southern YRV in August is remotely influenced by the Indian Ocean basin mode (IOBM) SSTAs.Corresponding to June-July-August (JJA) IOBM warming condition,the number of HTEs was above normal,and corresponding to IOBM cooling conditions,the number of HTEs was below normal across the southern YRV in August.The results of this study indicate that the tropical IOBM warming triggered low-level anomalous anticyclonic circulation in the subtropical northwestern Pacific Ocean and southern China by emanating a warm Kelvin wave in August.In the southern YRV,the reduced rainfall and downward vertical motion associated with the anomalous low-level anticyclonic circulation led to the increase of HTE frequency in August.

  7. Approximating actual flows in physical infrastructure networks: the case of the Yangtze River Delta high-speed railway network

    Directory of Open Access Journals (Sweden)

    Zhang Weiyang

    2016-03-01

    Full Text Available Previous empirical research on urban networks has used data on infrastructure networks to guesstimate actual inter-city flows. However, with the exception of recent research on airline networks in the context of the world city literature, relatively limited attention has been paid to the degree to which the outline of these infrastructure networks reflects the actual flows they undergird. This study presents a method to improve our estimation of urban interaction in and through infrastructure networks by focusing on the example of passenger railways, which is arguably a key potential data source in research on urban networks in metropolitan regions. We first review common biases when using infrastructure networks to approximate actual inter-city flows, after which we present an alternative approach that draws on research on operational train scheduling. This research has shown that ‘dwell time’ at train stations reflects the length of the alighting and boarding process, and we use this insight to estimate actual interaction through the application of a bimodal network projection function. We apply our method to the high-speed railway (HSR network within the Yangtze River Delta (YRD region, discuss the difference between our modelled network and the original network, and evaluate its validity through a systemic comparison with a benchmark dataset of actual passenger flows.

  8. Ecological Niche Modeling Identifies Fine-Scale Areas at High Risk of Dengue Fever in the Pearl River Delta, China

    Directory of Open Access Journals (Sweden)

    Qiaoxuan Li

    2017-06-01

    Full Text Available Dengue fever (DF is one of the most common and rapidly spreading mosquito-borne viral diseases in tropical and subtropical regions. In recent years, this imported disease has posed a serious threat to public health in China, especially in the Pearl River Delta (PRD. Although the severity of DF outbreaks in the PRD is generally associated with known risk factors, fine scale assessments of areas at high risk for DF outbreaks are limited. We built five ecological niche models to identify such areas including a variety of climatic, environmental, and socioeconomic variables, as well as, in some models, extracted principal components. All the models we tested accurately identified the risk of DF, the area under the receiver operating characteristic curve (AUC were greater than 0.8, but the model using all original variables was the most accurate (AUC = 0.906. Socioeconomic variables had a greater impact on this model (total contribution 55.27% than climatic and environmental variables (total contribution 44.93%. We found the highest risk of DF outbreaks on the border of Guangzhou and Foshan (in the central PRD, and in northern Zhongshan (in the southern PRD. Our fine-scale results may help health agencies to focus epidemic monitoring tightly on the areas at highest risk of DF outbreaks.

  9. Historical patterns of river stage and fish communities as criteria for operations of dams on the Illinois River

    Science.gov (United States)

    Koel, T.M.; Sparks, R.E.

    2002-01-01

    The hydrologic regime of the Illinois River has been altered over the past 100 years. Locks and dams regulate water surface elevations and flow, enabling commercial navigation to continue year round. This study relates changes in water surface elevation to fish abundance in the river, and establishes target criteria for operating locks and dams. Using longterm records of daily river stage, we identified ecologically meaningful hydrological parameters for eight gage locations along the Illinois River. Inter-annual variability of a long-term fisheries dataset beginning in 1957 was related to variability in stage, flood and recession duration, frequency, timing, and rate of change of water levels. Reversals in water surface elevation, maximum stage levels, and lenght of the spring flood were the most important parameters influencing abundance of age-zero fishes in annual collections. Smallmouth buffalo (Ictiobus bubalus), black crappie (Pomoxis nigromaculatus), freshwater drum (Aplodinotus grunneins), and white bass (Morone chrysops) were most abundant in samples during years that approximated the natural water level regime. Of the 33 hydrologic parameters evaluated for the entire water year from an Illinois River gage site on La Grange Reach, all except average stage in January and Julian date (JD) of maximum stage had moderate or high hydrologic alteration based on the historical range of variation (RVA). The highest degree of hydrologic alteration was for minimum stage levels (1-day, 3-day, and 7-day), rate-of-rise, and rate-of-fall. Other parameters that have been severely altered were 30-day minimum stage, 90-day maximum stage, and the annual number of water level reversals. Operations of the La Grange and Peoria locks and dams could be modified so water level variability would approximate that of the late 1800s, when fish and wildlife resources were abundant. The water regime could be regulated to maintain navigation and improve conditions for native plants and

  10. The Carmans River Story

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — In this study, undertaken as an independent project at Bellport High School, the authors have attempted to provide a historical description of the Carmans River area...

  11. Sandy River Delta Habitat Restoration Project, Annual Report 2001.

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, Virginia; Dobson, Robin L.

    2002-11-01

    The Sandy River Delta is located at the confluence of the Sandy and Columbia Rivers, just east of Troutdale, Oregon. It comprises about 1,400 land acres north of Interstate 84, managed by the USDA Forest Service, and associated river banks managed by the Oregon Division of State Lands. Three islands, Gary, Flag and Catham, managed by Metro Greenspaces and the State of Oregon lie to the east, the Columbia River lies to the north and east, and the urbanized Portland metropolitan area lies to the west across the Sandy River. Sandy River Delta was historically a wooded, riparian wetland with components of ponds, sloughs, bottomland woodland, oak woodland, prairie, and low and high elevation floodplain. It has been greatly altered by past agricultural practices and the Columbia River hydropower system. Restoration of historic landscape components is a primary goal for this land. The Forest Service is currently focusing on restoration of riparian forest and wetlands. Restoration of open upland areas (meadow/prairie) would follow substantial completion of the riparian and wetland restoration. The Sandy River Delta is a former pasture infested with reed canary grass, blackberry and thistle. The limited over story is native riparian species such as cottonwood and ash. The shrub and herbaceous layers are almost entirely non-native, invasive species. Native species have a difficult time naturally regenerating in the thick, competing reed canary grass, Himalayan blackberry and thistle. A system of drainage ditches installed by past owners drains water from historic wetlands. The original channel of the Sandy River was diked in the 1930's, and the river diverted into the ''Little Sandy River''. The original Sandy River channel has subsequently filled in and largely become a slough. The FS acquired approximately 1,400 acres Sandy River Delta (SRD) in 1991 from Reynolds Aluminum (via the Trust for Public Lands). The Delta had been grazed for many years

  12. Oxidative mitigation of aquatic methane emissions in large Amazonian rivers.

    Science.gov (United States)

    Sawakuchi, Henrique O; Bastviken, David; Sawakuchi, André O; Ward, Nicholas D; Borges, Clovis D; Tsai, Siu M; Richey, Jeffrey E; Ballester, Maria Victoria R; Krusche, Alex V

    2016-03-01

    The flux of methane (CH4 ) from inland waters to the atmosphere has a profound impact on global atmospheric greenhouse gas (GHG) levels, and yet, strikingly little is known about the dynamics controlling sources and sinks of CH4 in the aquatic setting. Here, we examine the cycling and flux of CH4 in six large rivers in the Amazon basin, including the Amazon River. Based on stable isotopic mass balances of CH4 , inputs and outputs to the water column were estimated. We determined that ecosystem methane oxidation (MOX) reduced the diffusive flux of CH4 by approximately 28-96% and varied depending on hydrologic regime and general geochemical characteristics of tributaries of the Amazon River. For example, the relative amount of MOX was maximal during high water in black and white water rivers and minimal in clear water rivers during low water. The abundance of genetic markers for methane-oxidizing bacteria (pmoA) was positively correlated with enhanced signals of oxidation, providing independent support for the detected MOX patterns. The results indicate that MOX in large Amazonian rivers can consume from 0.45 to 2.07 Tg CH4 yr(-1) , representing up to 7% of the estimated global soil sink. Nevertheless, climate change and changes in hydrology, for example, due to construction of dams, can alter this balance, influencing CH4 emissions to atmosphere.

  13. Short-Term Effects of the 2008 High-Flow Experiment on Macroinvertebrates in Colorado River Below Glen Canyon Dam, Arizona

    Science.gov (United States)

    Rosi-Marshall, Emma J.; Kennedy, Theodore A.; Kincaid, Dustin W.; Cross, Wyatt F.; Kelly, Holly A.W.; Behn, Kathrine A.; White, Tyler; Hall, Robert O.; Baxter, Colden V.

    2010-01-01

    Glen Canyon Dam has dramatically altered the physical environment (especially discharge regime, water temperatures, and sediment inputs) of the Colorado River. High-flow experiments (HFE) that mimic one aspect of the natural hydrograph (floods) were implemented in 1996, 2004, and 2008. The primary goal of these experiments was to increase the size and total area of sandbar habitats that provide both camping sites for recreational users and create backwaters (areas of stagnant flow in the lee of return-current eddies) that may be important as rearing habitat for native fish. Experimental flows might also positively or negatively alter the rainbow trout (Oncorhynchus mykiss) sport fishery in the clear tailwater reach below Glen Canyon Dam, Ariz., and native fish populations in downstream reaches (for example, endangered humpback chub, Gila cypha) through changes in available food resources. We examined the short-term response of benthic macroinvertebrates to the March 2008 HFE at three sites [river mile 0 (RM 0, 15.7 miles downriver from the dam), RM 62, and RM 225] along the Colorado River downstream from Glen Canyon Dam by sampling immediately before and then 1, 7, 14, and 30 days after the HFE. We selected these sites because of their importance to management; RM 0 has a valuable trout fishery, and RM 62 is the location of the largest population of the endangered humpback chub in the Grand Canyon. In addition to the short-term collection of samples, as part of parallel investigations, we collected 3 years of monthly (quarterly for RM 62) benthic macroinvertebrate samples that included 15 months of post-HFE data for all three sites, but processing of the samples is only complete for one site (RM 0). At RM 0, the HFE caused an immediate 1.75 g AFDM/m2 (expressed as grams ash-free dry mass, or AFDM) reduction of macroinvertebrate biomass that was driven by significant reductions in the biomass of the two dominant taxa in this reach-Potamopyrgus antipodarum (New

  14. Alteration in cardiac uncoupling proteins and eNOS gene expression following high-intensity interval training in favor of increasing mechanical efficiency

    OpenAIRE

    Ali Asghar Fallahi; Shahnaz Shekarfroush; Mostafa Rahimi; Amirhossain Jalali; Ali Khoshbaten

    2016-01-01

    Objective(s): High-intensity interval training (HIIT) increases energy expenditure and mechanical energy efficiency. Although both uncoupling proteins (UCPs) and endothelial nitric oxide synthase (eNOS) affect the mechanical efficiency and antioxidant capacity, their effects are inverse. The aim of this study was to determine whether the alterations of cardiac UCP2, UCP3, and eNOS mRNA expression following HIIT are in favor of increased mechanical efficiency or decreased oxidative stress. Mat...

  15. Controls on dryland mountain landscape development along the NW Saharan desert margin: Insights from Quaternary river terrace sequences (Dadès River, south-central High Atlas, Morocco)

    Science.gov (United States)

    Stokes, M.; Mather, A. E.; Belfoul, M.; Faik, F.; Bouzid, S.; Geach, M. R.; Cunha, P. P.; Boulton, S. J.; Thiel, C.

    2017-06-01

    This study documents river terraces from upstream reaches of the Dadès River, a major fluvial system draining the south-central High Atlas Mountains. Terraces occur as straths with bedrock bases positioned at 10 m altitudinal intervals up to 40 m (T1-T5) above the valley floor, becoming less common between 50 and 140 m. The rock strength, stratigraphy and structure of the mountain belt influences terrace distribution. Terraces are absent in river gorges of structurally thickened limestone; whilst well-developed, laterally continuous terraces (T1-T4) form along wide valleys occupying syncline structures dominated by weaker interbedded limestone-mudstone. Terrace staircases develop in confined canyons associated with weaker lithologies and influence from structural dip and stratigraphic configuration. Terraces comprise a bedrock erosion surface overlain by fluvial conglomerates, rare overbank sands and colluvium. This sequence with some OSL/IRSL age control, suggests terrace formation over a 100 ka climate cycle with valley floor aggradation during full glacials and incision during glacial-interglacial transitions. This integrates with other archives (e.g. lakes, glaciers, dunes), appearing typical of landscape development along the NW Saharan margin south of the High Atlas, and similar to patterns in the western-southern Mediterranean. The 100 ka climate cycle relationship suggests that the terrace sequence documents Late-Middle Pleistocene landscape development. Consistent altitudinal spacing of terraces and their distribution throughout the orogen suggests sustained base-level lowering linked to uplift-exhumation of the High Atlas. Low incision rates (<0.2 mm a-1) and general absence of terrace deformation suggests dominance of isostatically driven base-level lowering with relief generation being Early Pleistocene or older.

  16. Improving Understanding of Glacier Melt Contribution to High Asian River Discharge through Collaboration and Capacity Building with High Asian CHARIS Partner Institutions

    Science.gov (United States)

    Armstrong, Richard; Brodzik, Mary Jo; Armstrong, Betsy; Barrett, Andrew; Fetterer, Florence; Hill, Alice; Jodha Khalsa, Siri; Racoviteanu, Adina; Raup, Bruce; Rittger, Karl; Williams, Mark; Wilson, Alana; Ye, Qinghua

    2017-04-01

    The Contribution to High Asia Runoff from Ice & Snow (CHARIS) project uses remote sensing data combined with modeling from 2000 to the present to improve proportional estimates of melt from glaciers and seasonal snow surfaces. Based at the National Snow and Ice Data Center (NSIDC), University of Colorado, Boulder, USA, the CHARIS project objectives are twofold: 1) capacity-building efforts with CHARIS partners from eight High Asian countries to better forecast future availability and vulnerability of water resources in the region, and 2) improving our ability to systematically assess the role of glaciers and seasonal snow in the freshwater resources of High Asia. Capacity-building efforts include working with CHARIS partners from Bhutan, Nepal, India, Pakistan, Afghanistan, Kazakhstan, Kyrgyzstan and Tajikistan. Our capacity-building activities include training, data sharing, supporting fieldwork, graduate student education and infrastructure development. Because of the scarcity of in situ data in this High Asian region, we are using the wealth of available remote sensing data to characterize digital elevation, daily maps of fractional snow-cover, annual maps of glacier and permanent snow cover area and downscaled reanalysis temperature data in snow melt models to estimate the relative proportions of river runoff from glacierized and seasonally snow-covered surfaces. Current collaboration with Qinghua Ye, visiting scientist at NSIDC from the Institute of Tibetan Plateau Research, CAS, focuses on remote sensing methods to detect changes in the mountain cryosphere. Collaboration with our Asian partners supports the systematic analysis of the annual cycle of seasonal snow and glacier ice melt across the High Mountain Asia region. With our Asian partners, we have derived reciprocal benefits, learning from their specialized local knowledge and obtaining access to their in situ data. We expect that the improved understanding of runoff from snow and glacier surfaces will

  17. Fish assemblage shifts in the Powder River of Wyoming: an unregulated prairie river system previously considered to be relatively pristine.

    Science.gov (United States)

    Senecal, Anna C.; Walters, Annika W.; Hubert, Wayne A.

    2016-01-01

    Wyoming’s Powder River is considered an example of a pristine prairie river system. While the river hosts a largely native fish assemblage and remains unimpounded over its 1,146-km course to the Yellowstone River confluence, the hydrologic regime has been altered through water diversion for agriculture and natural gas extraction and there has been limited study of fish assemblage structure. We analyzed fish data collected from the mainstem Powder River in Wyoming between 1896 and 2008. Shifts in presence/absence and relative abundance of fish species, as well as fish assemblage composition, were assessed among historical and recent samples. The recent Powder River fish assemblage was characterized by increased relative abundances of sand shiner Notropis stramineus and plains killifish Fundulus zebrinus, and decreases in sturgeon chub Macrhybopsis gelida. Shifts in fish species relative abundance are linked to their reproductive ecology with species with adhesive eggs generally increasing in relative abundance while those with buoyant drifting eggs are decreasing. Assemblage shifts could be the result of landscape level changes, such as the loss of extreme high and low flow events and changing land use practices.

  18. High estrogen concentrations in receiving river discharge from a concentrated livestock feedlot.

    Science.gov (United States)

    Chen, Te-San; Chen, Ting-Chien; Yeh, Kuei-Jyum C; Chao, How-Ran; Liaw, Ean-Tun; Hsieh, Chi-Ying; Chen, Kuan-Chung; Hsieh, Lien-Te; Yeh, Yi-Lung

    2010-07-15

    Environmental estrogenic chemicals interrupt endocrine systems and generate reproductive abnormalities in wildlife, especially natural and synthetic estrogenic steroid hormones such as 17beta-estradiol (E2), estrone (E1), estriol (E3), 17alpha-ethynylestradiol (EE2), and diethylstilbestrol (DES). Concentrated animal feedlot operations (CAFOs) are of particular concern since large amounts of naturally excreted estrogens are discharged into aquatic environments. This study investigated E2, E1, E3, EE2, and DES with high performance liquid chromatography/tandem mass (HPLC-MS/MS) analyses along Wulo Creek in southern Taiwan, near a concentrated livestock feedlot containing 1,030,000 broiler chickens, 934,000 laying hens, 85,000 pigs, and 1500 cattle. Sampling was performed from December 2008 to May 2009, in which 54 samples were collected. Experimental results indicate that concentrations of EE2 were lower than the limit of detection (LOD), and concentrations of DES were only detected twice. Concentrations ranged from 7.4 to 1267 ng/L for E1, from not detected (ND) to 313.6 ng/L for E2, and from ND to 210 ng/L for E3. E1 had the highest average mass fraction (72.2 + or - 3.6%), which was significantly higher than E3 (16.2 + or - 1.7%) and E2 (11.5 + or - 2.6%). Additionally, the mean E2 equivalent quotient (EEQ) ranged from 17.3 to 137.9 ng-E2/L. Despite having a markedly lower concentration than E1, E2 more significantly contributed (52.4 + or - 6.0%) EEQ than E1 (19.7 + or - 3.5%). Moreover, the concentrations of E2, E1, and E3 upstream were significantly higher than concentrations downstream, suggesting a high attenuation effect and fast degradation in the study water. Most concentrations in winter season were higher than those of spring season due to the low dilution effect and low microbial activity in the winter season. Based on the results of this study, we recommend further treatment of the wastewater discharge from the feedlot.

  19. Persistence of effects of high sediment loading in a salmon-bearing river, northern California

    Science.gov (United States)

    Madej, M.A.; Ozaki, V.

    2009-01-01

    Regional high-magnitude rainstorms have produced several large floods in north coastal California during the last century, which resulted in extensive massmovement activity and channel aggradation. Channel monitoring in Redwood Creek, through the use of cross-sectional surveys, thalweg profi les, and pebble counts, has documented the persistence and routing of channel-stored sediment following these large floods in the 1960s and 1970s. Channel response varied on the basis of timing of peak aggradation. Channel-stored sediment was evacuated rapidly from the upstream third of the Redwood Creek channel, and the channel bed stabilized by 1985 as the bed coarsened. Currently only narrow remnants of flood deposits remain and are well vegetated. In the downstream reach, channel aggradation peaked in the 1990s, and the channel is still incising. Channel-bed elevations throughout the watershed showed an approximate exponential decrease with time, but decay rates were highest in areas with the thickest flood deposits. Pool frequencies and depths generally increased from 1977 to 1995, as did median residual water depths, but a 10 yr flood in 1997 resulted in a moderate reversal of this trend. Channel aggradation generated during 25 yr return interval floods has persisted in Redwood Creek for more than 30 yr and has impacted many life cycles of salmon. Watershed restoration work is currently focused on correcting erosion problems on hillslopes to reduce future sediment supply to Redwood Creek instead of attempting in-channel manipulations. ?? 2009 Geological Society of America.

  20. Cry me a river: identifying the behavioral consequences of extremely high-stakes interpersonal deception.

    Science.gov (United States)

    Ten Brinke, Leanne; Porter, Stephen

    2012-12-01

    Deception evolved as a fundamental aspect of human social interaction. Numerous studies have examined behavioral cues to deception, but most have involved inconsequential lies and unmotivated liars in a laboratory context. We conducted the most comprehensive study to date of the behavioral consequences of extremely high-stakes, real-life deception--relative to comparable real-life sincere displays--via 3 communication channels: speech, body language, and emotional facial expressions. Televised footage of a large international sample of individuals (N = 78) emotionally pleading to the public for the return of a missing relative was meticulously coded frame-by-frame (30 frames/s for a total of 74,731 frames). About half of the pleaders eventually were convicted of killing the missing person on the basis of overwhelming evidence. Failed attempts to simulate sadness and leakage of happiness revealed deceptive pleaders' covert emotions. Liars used fewer words but more tentative words than truth-tellers, likely relating to increased cognitive load and psychological distancing. Further, each of these cues explained unique variance in predicting pleader sincerity.

  1. High-resolution flow characterization close to the sediment-water interface in a run of the river reservoir

    Science.gov (United States)

    Brand, Andreas; Noss, Christian

    2017-05-01

    A bistatic high-resolution acoustic profiler was used in order to characterize the lowermost boundary layer of a run of the river reservoir. The profiler allows determining the statistics of the three-dimensional flow field at a single point (sweet spot) as well as the measurement of the time averaged flow velocity profiles at 1 mm resolution around the sweet spot. Therefore, in addition to the flow statistics provided by single point acoustic Doppler profilers, mixing coefficients as well as production of turbulent kinetic energy can be calculated using a single device. Fitting of semiempirical relations to observed cospectra allowed eliminating artifacts as they result from coordinate system rotation during calculation of Reynolds stress profiles at millimeter resolution. While most parameters showed characteristics of a constant stress layer, length scales indicated anisotropy of the turbulent flow. Under these anisotropic near wall conditions, we found that the use of the commonly accepted Kolmogorov constants for the determination of dissipation rates using the inertial dissipation method is not valid any more. Instead, these constants vary with distance from the sediment water interface. We provide evidence that coefficients determined by numerical simulations are the appropriate choice also in field applications. In addition we resolved the viscous boundary layer close to the sediment-water interface in high resolution (1 mm) profiles and identified a double logarithmic layer above 1.5 cm at one location. The discrepancy of the scales as well as the double logarithmic layer suggests the existence of roughness elements upstream of the measurement sites.

  2. High-resolution precipitation data derived from dynamical downscaling using the WRF model for the Heihe River Basin, northwest China

    Science.gov (United States)

    Zhang, Xuezhen; Xiong, Zhe; Zheng, Jingyun; Ge, Quansheng

    2017-01-01

    The community of climate change impact assessments and adaptations research needs regional high-resolution (spatial) meteorological data. This study produced two downscaled precipitation datasets with spatial resolutions of as high as 3 km by 3 km for the Heihe River Basin (HRB) from 2011 to 2014 using the Weather Research and Forecast (WRF) model nested with Final Analysis (FNL) from the National Center for Environmental Prediction (NCEP) and ERA-Interim from the European Centre for Medium-Range Weather Forecasts (ECMWF) (hereafter referred to as FNLexp and ERAexp, respectively). Both of the downscaling simulations generally reproduced the observed spatial patterns of precipitation. However, users should keep in mind that the two downscaled datasets are not exactly the same in terms of observations. In comparison to the remote sensing-based estimation, the FNLexp produced a bias of heavy precipitation centers. In comparison to the ground gauge-based measurements, for the warm season (May to September), the ERAexp produced more precipitation (root-mean-square error (RMSE) = 295.4 mm, across the 43 sites) and more heavy rainfall days, while the FNLexp produced less precipitation (RMSE = 115.6 mm) and less heavy rainfall days. Both the ERAexp and FNLexp produced considerably more precipitation for the cold season (October to April) with RMSE values of 119.5 and 32.2 mm, respectively, and more heavy precipitation days. Along with simulating a higher number of heavy precipitation days, both the FNLexp and ERAexp also simulated stronger extreme precipitation. Sensitivity experiments show that the bias of these simulations is much more sensitive to micro-physical parameterizations than to the spatial resolution of topography data. For the HRB, application of the WSM3 scheme may improve the performance of the WRF model.

  3. MECHANISMS OF PHASE FORMATION IN THE VITRIFICATION OF HIGH-FERROUS SAVANNAH RIVER SITE SB2 HLW SLUDGE SURROGATE - 9300

    Energy Technology Data Exchange (ETDEWEB)

    Marra, J

    2008-08-27

    Phase formation mechanisms associated with the vitrification of high-ferrous Savannah River Site (SRS) Sludge Batch 2 (SB2) high level waste surrogate were studied by infrared spectroscopy (IRS) and X-ray diffraction (XRD). Two mixtures at 50 wt% waste loading with commercially available Frit 320 (Li{sub 2}O - 8 wt %, B{sub 2}O{sub 3} - 8 wt %, Na{sub 2}O - 12 wt %, SiO{sub 2} - 72 wt %) and batch chemicals (LiOH {center_dot} H{sub 2}O, H{sub 3}BO{sub 3}, NaNO{sub 3}, SiO{sub 2}) to represent the frit formulation were prepared as slurries with a water content of {approx}50 wt%. The mixtures were air-dried at a temperature of 115 C and heat-treated at 500, 700, 900, 1000, 1100, 1200, and 1300 C for 1 hr at each temperature. Infrared spectra and XRD patterns of the products produced at each temperature were recorded. In both mixtures prepared using frit and batch chemicals to represent the frit, phase formation reactions were completed within the temperature range between 900 and 1000 C. However, residual quartz was still present in glass produced from the mixture with batch chemicals even at 1100 C. Although, the phase composition and structure of the glassy products obtained from both mixtures at temperatures over 1000 C were similar, the products obtained from the mixture using actual frit were more homogeneous than those from the mixture with batch chemicals. Thus, the use of frit rather than batch chemicals reduced the temperature range of phase formation and provided for production of higher quality glass.

  4. High spatial resolution geochemistry and textural characteristics of 'microtektite' glass spherules in proximal Cretaceous-Paleogene sections: Insights into glass alteration patterns and precursor melt lithologies

    Science.gov (United States)

    Belza, Joke; Goderis, Steven; Smit, Jan; Vanhaecke, Frank; Baert, Kitty; Terryn, Herman; Claeys, Philippe

    2015-03-01

    Using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), we have conducted spatially resolved trace element analysis on fresh, unaltered microtektite glasses linked to the Cretaceous-Paleogene (K-Pg) boundary Chicxulub crater and on their surrounding alteration phases. This unique approach offers the opportunity to study in situ and at high spatial resolution both the mixing of different target lithologies and the variation of the major and trace element budget during the alteration process. In addition, two-dimensional element distribution maps reveal important geochemical information beyond the capabilities of single spot laser drilling. Glasses from two localities in opposite quadrants from the source crater were studied. At the Beloc locality (Haiti), the glass population is dominated by the presence of yellow high-Ca glass and black andesitic glass formed by admixture of carbonate/dolomite/anhydrite platform lithologies with crystalline basement. These glasses alter according to the well-established hydration-palagonitization model postulated for mafic volcanic glasses. REEs become progressively leached from the glass to below the detection limit for the applied spot size, while immobile Zr, Hf, Nb, and Ta passively accumulate in the process exhibiting both inter-element ratios and absolute concentrations similar to those for the original glass. In contrast, The Arroyo El Mimbral locality (NE Mexico) is characterized by abundant green glass fragments high in Si, Al and alkalis, and low in Mg, Ca, Fe. Low Si black glass is less abundant though similar in composition to the black glass variety at Beloc. The alteration pattern of high-Si, Al green glass at the Mimbral locality is more complex, including numerous competing reaction processes (ion-exchange, hydration, dissolution, and secondary mineral precipitation) generally controlled by the pH and composition of the surrounding fluid. All green, high-Si, Al glasses are hydrated and

  5. Physico-chemical and biological study of the river Chittar at Courtallam, Tamil Nadu (India).