Network based on statistical multiplexing for event selection and event builder systems in high energy physics experiments

International Nuclear Information System (INIS)

Calvet, D.

2000-03-01

Systems for on-line event selection in future high energy physics experiments will use advanced distributed computing techniques and will need high speed networks. After a brief description of projects at the Large Hadron Collider, the architectures initially proposed for the Trigger and Data AcQuisition (TD/DAQ) systems of ATLAS and CMS experiments are presented and analyzed. A new architecture for the ATLAS T/DAQ is introduced. Candidate network technologies for this system are described. This thesis focuses on ATM. A variety of network structures and topologies suited to partial and full event building are investigated. The need for efficient networking is shown. Optimization techniques for high speed messaging and their implementation on ATM components are described. Small scale demonstrator systems consisting of up to 48 computers (∼1:20 of the final level 2 trigger) connected via ATM are described. Performance results are presented. Extrapolation of measurements and evaluation of needs lead to a proposal of implementation for the main network of the ATLAS T/DAQ system. (author)

The Design and Analysis of Virtual Network Configuration for a Wireless Mobile ATM Network

OpenAIRE

Bush, Stephen F.

1999-01-01

This research concentrates on the design and analysis of an algorithm referred to as Virtual Network Configuration (VNC) which uses predicted future states of a system for faster network configuration and management. VNC is applied to the configuration of a wireless mobile ATM network. VNC is built on techniques from parallel discrete event simulation merged with constraints from real-time systems and applied to mobile ATM configuration and handoff. Configuration in a mobile network is a dyna...

CERNET - A high-speed packet-switching network

International Nuclear Information System (INIS)

Gerard, J.M.

1981-01-01

A general mesh-structured high-speed computer network has been designed and built. This network provides communication between any pair of connected user computers over distances of upto 6 km and at line speeds of 1 to 5 Mbit/second. The network is composed of a communication subnet providing a datagram service, complemented by tasks in the connected machines to implement an end-to-end logical link protocol. Details are given of the overall structure as well as the specific modules of which the system is composed. (orig.)

High speed network sampling

OpenAIRE

Rindalsholt, Ole Arild

2005-01-01

Master i nettverks- og systemadministrasjon Classical Sampling methods play an important role in the current practice of Internet measurement. With today’s high speed networks, routers cannot manage to generate complete Netflow data for every packet. They have to perform restricted sampling. This thesis summarizes some of the most important sampling schemes and their applications before diving into an analysis on the effect of sampling Netflow records.

High speed VLSI neural network for high energy physics

NARCIS (Netherlands)

Masa, P.; Masa, P.; Hoen, K.; Hoen, Klaas; Wallinga, Hans

1994-01-01

A CMOS neural network IC is discussed which was designed for very high speed applications. The parallel architecture, analog computing and digital weight storage provides unprecedented computing speed combined with ease of use. The circuit classifies up to 70 dimensional vectors within 20

Knowledge-Based Multiple Access Protocol in Broadband Wireless ATM Networks

DEFF Research Database (Denmark)

Liu, Hong; Gliese, Ulrik Bo; Dittmann, Lars

1999-01-01

In this paper, we propose a knowledge-based multiple access protocol for the extension of wireline ATM to wireless networks. The objective is to enable effecient transmission of all kinds of ATM traffic in the wireless channel with guaranteed QoS.The proposed protocol utilixes knowledge of the main...... guaranteed QoS requirements to a variety of ATM applications....

Flow enforcement algorithms for ATM networks

DEFF Research Database (Denmark)

Dittmann, Lars; Jacobsen, Søren B.; Moth, Klaus

1991-01-01

Four measurement algorithms for flow enforcement in asynchronous transfer mode (ATM) networks are presented. The algorithms are the leaky bucket, the rectangular sliding window, the triangular sliding window, and the exponentially weighted moving average. A comparison, based partly on teletraffic...

Multiple access protocol for supporting multimedia services in wireless ATM networks

DEFF Research Database (Denmark)

Liu, Hong; Dittmann, Lars; Gliese, Ulrik Bo

1999-01-01

The furture broadband wireless asynchronous transfer mode (ATM) networks must provide seamless extension of multimedia services from the wireline ATM networks. This requires an effecient wireless access protocol to fulfill varying Quality-og-Service (QoS) requirements for multimedia applications....... In this paper, we propose a multiple access protocol using centralized and distributed channel access control techniques to provide QoS guarantees for multimedia services by taking advantage of the characteristics of different kinds of ATM traffics. Multimedia traffic, including constant bit rate (CBR...

A 2.5 gb/s GaAs ATM Mux Demux ASIC

DEFF Research Database (Denmark)

Madsen, Jens Kargaard; Lassen, Peter Stuhr

1995-01-01

This paper describes the design and implementation of a high speed GaAs ATM Mux Demur ASIC (AMDA) which is the key element in a high speed ATM Add-Drop unit. This unit is used in a new distributed ATM multiplexing-demultiplexing architecture for broadband switching systems. The Add-Drop unit...

Experimental high-speed network

Science.gov (United States)

McNeill, Kevin M.; Klein, William P.; Vercillo, Richard; Alsafadi, Yasser H.; Parra, Miguel V.; Dallas, William J.

1993-09-01

Many existing local area networking protocols currently applied in medical imaging were originally designed for relatively low-speed, low-volume networking. These protocols utilize small packet sizes appropriate for text based communication. Local area networks of this type typically provide raw bandwidth under 125 MHz. These older network technologies are not optimized for the low delay, high data traffic environment of a totally digital radiology department. Some current implementations use point-to-point links when greater bandwidth is required. However, the use of point-to-point communications for a total digital radiology department network presents many disadvantages. This paper describes work on an experimental multi-access local area network called XFT. The work includes the protocol specification, and the design and implementation of network interface hardware and software. The protocol specifies the Physical and Data Link layers (OSI layers 1 & 2) for a fiber-optic based token ring providing a raw bandwidth of 500 MHz. The protocol design and implementation of the XFT interface hardware includes many features to optimize image transfer and provide flexibility for additional future enhancements which include: a modular hardware design supporting easy portability to a variety of host system buses, a versatile message buffer design providing 16 MB of memory, and the capability to extend the raw bandwidth of the network to 3.0 GHz.

Network Based High Speed Product Innovation

DEFF Research Database (Denmark)

Lindgren, Peter

In the first decade of the 21st century, New Product Development has undergone major changes in the way NPD is managed and organised. This is due to changes in technology, market demands, and in the competencies of companies. As a result NPD organised in different forms of networks is predicted...... to be of ever-increasing importance to many different kinds of companies. This happens at the same times as the share of new products of total turnover and earnings is increasing at unprecedented speed in many firms and industries. The latter results in the need for very fast innovation and product development...... - a need that can almost only be resolved by organising NPD in some form of network configuration. The work of Peter Lindgren is on several aspects of network based high speed product innovation and contributes to a descriptive understanding of this phenomenon as well as with normative theory on how NPD...

Teleoperation system using Asynchronous transfer mode, ATM network

International Nuclear Information System (INIS)

Mohd Dani Baba; A Nasoruddin Mohamad

1999-01-01

This paper examines the application of Asynchronous Transfer Mode (ATM) in a distributed industrial environment such as in teleoperation, which performs real time control manipulation from a remote location. In our study, two models of teleoperation are proposed; the first model is a point to point connection and the second model is through an ATM network. The performance results are analysed as to determine whether the two models can support the teleoperation traffics via simulation using commercial software design tool. (Author)

Design Issues for Traffic Management for the ATM UBR + Service for TCP Over Satellite Networks

Science.gov (United States)

Jain, Raj

1999-01-01

This project was a comprehensive research program for developing techniques for improving the performance of Internet protocols over Asynchronous Transfer Mode (ATM) based satellite networks. Among the service categories provided by ATM networks, the most commonly used category for data traffic is the unspecified bit rate (UBR) service. UBR allows sources to send data into the network without any feedback control. The project resulted in the numerous ATM Forum contributions and papers.

Hierarchical micro-mobility management in high-speed multihop access networks

Institute of Scientific and Technical Information of China (English)

TANG Bi-hua; MA Xiao-lei; LIU Yuan-an; GAO Jin-chun

2006-01-01

This article integrates the hierarchical micro-mobility management and the high-speed multihop access networks (HMAN), to accomplish the smooth handover between different access routers. The proposed soft handover scheme in the high-speed HMAN can solve the micro-mobility management problem in the access network. This article also proposes the hybrid access router (AR) advertisement scheme and AR selection algorithm, which uses the time delay and stable route to the AR as the gateway selection parameters. By simulation, the proposed micro-mobility management scheme can achieve high packet delivery fraction and improve the lifetime of network.

Efficient Bandwidth Allocation for Integrated Services in Broadband Wireless ATM Networks

DEFF Research Database (Denmark)

Liu, Hong; Dittmann, Lars; Gliese, Ulrik Bo

1999-01-01

An efficient bandwidth allocation scheme is proposed for supporting intergrated services in wireless ATM networks. These include CBR, VBR amd ABR types of traffic. The proposed scheme is based om A-PRMA for carrying ATM traffic in a dynamic TDMA type access system. It allows mobile users to adjust...

High-Speed Railways and Urban Networks in China

NARCIS (Netherlands)

Yang, Haoran

2018-01-01

Worldwide, High-Speed Railway (HSR) networks have been developed intensely over the last few decades, such as Tokyo-Osaka, the first HSR corridor in Japan, the TGV in France and the ICE in Germany. HSR has also experienced exponential growth in China so that currently China’s HSR networks are the

HIGH SPEED RAILWAY LINES – FUTURE PART OF CZECH RAILWAY NETWORK?

Directory of Open Access Journals (Sweden)

Lukáš Týfa

2017-08-01

Full Text Available The paper first describes high speed rail generally and explains the relationship between high speed and conventional railway networks (according to the vehicle types in operation on the network. The core of the paper is comprised of the methodology for choosing the best route for a railway line and its application to the high speed railway connection Praha – Brno. The Algorithm used assumes the existence of more route proposals, which could be different in terms of the operational conception, line routing or types of vehicles used. The optimal variant is the one with the lowest daily cost, which includes infrastructure and vehicle costs; investment and operational costs. The results from applying this model confirmed the assumption, that a dedicated high speed railway line, only for high speed trains, has the same or lower investment costs than a line for both high speed and conventional trains. Furthermore, a dedicated high line also has a lower cost for infrastructure maintenance but a higher cost for buying high speed multiple units.

High speed all optical networks

Science.gov (United States)

Chlamtac, Imrich; Ganz, Aura

1990-01-01

An inherent problem of conventional point-to-point wide area network (WAN) architectures is that they cannot translate optical transmission bandwidth into comparable user available throughput due to the limiting electronic processing speed of the switching nodes. The first solution to wavelength division multiplexing (WDM) based WAN networks that overcomes this limitation is presented. The proposed Lightnet architecture takes into account the idiosyncrasies of WDM switching/transmission leading to an efficient and pragmatic solution. The Lightnet architecture trades the ample WDM bandwidth for a reduction in the number of processing stages and a simplification of each switching stage, leading to drastically increased effective network throughputs. The principle of the Lightnet architecture is the construction and use of virtual topology networks, embedded in the original network in the wavelength domain. For this construction Lightnets utilize the new concept of lightpaths which constitute the links of the virtual topology. Lightpaths are all-optical, multihop, paths in the network that allow data to be switched through intermediate nodes using high throughput passive optical switches. The use of the virtual topologies and the associated switching design introduce a number of new ideas, which are discussed in detail.

Highball: A high speed, reserved-access, wide area network

Science.gov (United States)

Mills, David L.; Boncelet, Charles G.; Elias, John G.; Schragger, Paul A.; Jackson, Alden W.

1990-01-01

A network architecture called Highball and a preliminary design for a prototype, wide-area data network designed to operate at speeds of 1 Gbps and beyond are described. It is intended for applications requiring high speed burst transmissions where some latency between requesting a transmission and granting the request can be anticipated and tolerated. Examples include real-time video and disk-disk transfers, national filestore access, remote sensing, and similar applications. The network nodes include an intelligent crossbar switch, but have no buffering capabilities; thus, data must be queued at the end nodes. There are no restrictions on the network topology, link speeds, or end-end protocols. The end system, nodes, and links can operate at any speed up to the limits imposed by the physical facilities. An overview of an initial design approach is presented and is intended as a benchmark upon which a detailed design can be developed. It describes the network architecture and proposed access protocols, as well as functional descriptions of the hardware and software components that could be used in a prototype implementation. It concludes with a discussion of additional issues to be resolved in continuing stages of this project.

ATM and Internet protocol

CERN Document Server

Bentall, M; Turton, B

1998-01-01

Asynchronous Transfer Mode (ATM) is a protocol that allows data, sound and video being transferred between independent networks via ISDN links to be supplied to, and interpreted by, the various system protocols.ATM and Internet Protocol explains the working of the ATM and B-ISDN network for readers with a basic understanding of telecommunications. It provides a handy reference to everyone working with ATM who may not require the full standards in detail, but need a comprehensive guide to ATM. A substantial section is devoted to the problems of running IP over ATM and there is some discussion o

Multicast Performance Analysis for High-Speed Torus Networks

National Research Council Canada - National Science Library

Oral, S; George, A

2002-01-01

... for unicast-based and path-based multicast communication on high-speed torus networks. Software-based multicast performance results of selected algorithms on a 16-node Scalable Coherent Interface (SCI) torus are given...

Investment and Usage of New Technologies : Evidence from a Shared ATM Network

NARCIS (Netherlands)

Ferrari, S.; Verboven, F.L.; Degryse, H.A.

2007-01-01

When new technologies become available, it is not only essential that firms have the correct investment incentives, but often also that consumers make the proper usage decisions. This paper studies investment and usage in a shared ATM network. Be- cause all banks coordinate their ATM investment

Investment and Usage of New Technologies : Evidence from a Shared ATM Network

NARCIS (Netherlands)

Ferrari, S.; Verboven, F.L.; Degryse, H.A.

2008-01-01

When new technologies become available, it is not only essential that firms have the correct investment incentives, but often also that consumers make the proper usage decisions. This paper studies investment and usage in a shared ATM network. Be- cause all banks coordinate their ATM investment

ATM technology and beyond

Science.gov (United States)

Cheung, Nim K.

1993-01-01

Networks based on Asynchronous Transfer Mode (ATM) are expected to provide cost-effective and ubiquitous infrastructure to support broadband and multimedia services. In this paper, we give an overview of the ATM standards and its associated physical layer transport technologies. We use the experimental HIPPI-ATM-SONET (HAS) interface in the Nectar Gigabit Testbed to illustrate how one can use the SONET/ATM public network to provide transport for bursty gigabit applications.

Research in high speed fiber optics local area networks

Science.gov (United States)

Tobagi, F. A.

1986-01-01

The design of high speed local area networks (HSLAN) for communication among distributed devices requires solving problems in three areas: the network medium and its topology, the medium access control, and the network interface. Considerable progress was already made in the first two areas. Accomplishments are divided into two groups according to their theoretical or experimental nature. A brief summary is given.

Argonne National Laboratory high performance network support of APS experiments

International Nuclear Information System (INIS)

Knot, M.J.; McMahon, R.J.

1996-01-01

Argonne National Laboratory is currently positioned to provide access to high performance regional and national networks. Much of the impetus for this effort is the anticipated needs of the upcoming experimental program at the APS. Some APS collaborative access teams (CATs) are already pressing for network speed improvements and security enhancements. Requirements range from the need for high data rate, secure transmission of experimental data, to the desire to establish a open-quote open-quote virtual experimental environment close-quote close-quote at their home institution. In the near future, 155 megabit/sec (Mb/s) national and regional asynchronous transfer mode (ATM) networks will be operational and available to APS users. Full-video teleconferencing, virtual presence operation of experiments, and high speed, secure transmission of data are being tested and, in some cases, will be operational. We expect these efforts to enable a substantial improvement in the speed of processing experimental results as well as an increase in convenience to the APS experimentalist. copyright 1996 American Institute of Physics

A high-speed interconnect network using ternary logic

DEFF Research Database (Denmark)

Madsen, Jens Kargaard; Long, S. I.

1995-01-01

This paper describes the design and implementation of a high-speed interconnect network (ICN) for a multiprocessor system using ternary logic. By using ternary logic and a fast point-to-point communication technique called STARI (Self-Timed At Receiver's Input), the communication between...

Web Based ATM PVC Management

NARCIS (Netherlands)

van der Waaij, B.D.; Sprenkels, Ron; van Beijnum, Bernhard J.F.; Pras, Aiko

1998-01-01

This paper discusses the design of a public domain web based ATM PVC Management tool for the Dutch SURFnet research ATM network. The aim of this tool is to assists in the creation and deletion of PVCs through local and remote ATM network domains. The tool includes security mechanisms to restrict the

A hybrid medium access control for convergence of broadband wireless and wireline ATM networks

DEFF Research Database (Denmark)

Liu, Hong; Gliese, Ulrik Bo; Dittmann, Lars

2000-01-01

In this paper, we propose a hybrid medium access control protocol for supporting broadband integrated services in the wireless ATM networks. The integrated services include CBR, VBR and ABR traffic varying from low bit-rate to very high bit-rate. The proposed protocol is an excellent compromise...

Design and implementation of interface units for high speed fiber optics local area networks and broadband integrated services digital networks

Science.gov (United States)

Tobagi, Fouad A.; Dalgic, Ismail; Pang, Joseph

1990-01-01

The design and implementation of interface units for high speed Fiber Optic Local Area Networks and Broadband Integrated Services Digital Networks are discussed. During the last years, a number of network adapters that are designed to support high speed communications have emerged. This approach to the design of a high speed network interface unit was to implement package processing functions in hardware, using VLSI technology. The VLSI hardware implementation of a buffer management unit, which is required in such architectures, is described.

Parallel computing and networking; Heiretsu keisanki to network

Energy Technology Data Exchange (ETDEWEB)

Asakawa, E; Tsuru, T [Japan National Oil Corp., Tokyo (Japan); Matsuoka, T [Japan Petroleum Exploration Co. Ltd., Tokyo (Japan)

1996-05-01

This paper describes the trend of parallel computers used in geophysical exploration. Around 1993 was the early days when the parallel computers began to be used for geophysical exploration. Classification of these computers those days was mainly MIMD (multiple instruction stream, multiple data stream), SIMD (single instruction stream, multiple data stream) and the like. Parallel computers were publicized in the 1994 meeting of the Geophysical Exploration Society as a `high precision imaging technology`. Concerning the library of parallel computers, there was a shift to PVM (parallel virtual machine) in 1993 and to MPI (message passing interface) in 1995. In addition, the compiler of FORTRAN90 was released with support implemented for data parallel and vector computers. In 1993, networks used were Ethernet, FDDI, CDDI and HIPPI. In 1995, the OC-3 products under ATM began to propagate. However, ATM remains to be an interoffice high speed network because the ATM service has not spread yet for the public network. 1 ref.

Experiences with ATM in a multivendor pilot system at Forschungszentrum Julich

Science.gov (United States)

Kleines, H.; Ziemons, K.; Zwoll, K.

1998-08-01

The ATM technology for high speed serial transmission provides a new quality of communication by introducing novel features in a LAN environment, especially support of real time communication, of both LAN and WAN communication and of multimedia streams. In order to evaluate ATM for future DAQ systems and remote control systems as well as for a high speed picture archiving and communications system for medical images, Forschungszentrum Julich has build up a pilot system for the evaluation of ATM and standard low cost multimedia systems. It is a heterogeneous multivendor system containing a variety of switches and desktop solutions, employing different protocol options of ATM. The tests conducted in the pilot system revealed major difficulties regarding stability, interoperability and performance. The paper presents motivations, layout and results of the pilot system. Discussion of results concentrates on performance issues relevant for realistic applications, e.g., connection to a RAID system via NFS over ATM.

Impact of Loss Synchronization on Reliable High Speed Networks: A Model Based Simulation

Directory of Open Access Journals (Sweden)

Suman Kumar

2014-01-01

Full Text Available Contemporary nature of network evolution demands for simulation models which are flexible, scalable, and easily implementable. In this paper, we propose a fluid based model for performance analysis of reliable high speed networks. In particular, this paper aims to study the dynamic relationship between congestion control algorithms and queue management schemes, in order to develop a better understanding of the causal linkages between the two. We propose a loss synchronization module which is user configurable. We validate our model through simulations under controlled settings. Also, we present a performance analysis to provide insights into two important issues concerning 10 Gbps high speed networks: (i impact of bottleneck buffer size on the performance of 10 Gbps high speed network and (ii impact of level of loss synchronization on link utilization-fairness tradeoffs. The practical impact of the proposed work is to provide design guidelines along with a powerful simulation tool to protocol designers and network developers.

Performance Analysis of Quality-of-Service Controls in a Cell-Cluster-Based Wireless ATM Network

Energy Technology Data Exchange (ETDEWEB)

Cho, Young Jong [Ajou University, Suwon (Korea, Republic of)

1997-04-01

In this paper, an efficient cell-cluster-based call control scheme with guaranteed quality-of-service(QoS) provision ing is presented for next generation wireless ATM networks and its performance is mathematically analyzed using the open queuing network. With the cell-cluster-based call control, at the time a mobile connection is admitted to the network, a virtual cell is constructed by choosing a group of neighboring base stations to which the call may probabilistic ally hand over and by assigning to the call a collection of virtual paths between the base stations. Within a micro cell/pico cell environment, it is seen that the cell-cluster-based call control can support effectively a very high rate of handovers, provides very high system capacity, and guarantees a high degree of frequency reuse over the same geographical region without requiring the intervention of the network call control processor each time a handover occurs. But since mobiles, once admitted, are free to roam within the virtual cell, congestion condition occurs in which the number of calls to be handled by one base station exceeds the cell sites` capacity of radio channel and consequently a predefined QoS provision cannot be guaranteed. So, there must be a call admission control function to limit the number of calls existing in a cell-cluster such that required QoS objectives are met. As call acceptance criteria for constant-bit-rate or realtime variable-bit-rate ATM connections, we define four mobile QoS metrics: new-call blocking probability, wireless channel utilization efficiency, congestion probability and normalized average congestion duration. In addition, for QoS provision ing to available-bit-rate, unspecified-bit-rate or non-realtime variable-bit-rate connections, we further define another QoS metric, the minimum threshold breaking probability. By using the open network queuing model, we derive closed form expressions for the five QoS metrics defined above and show that they can be

Network based on statistical multiplexing for event selection and event builder systems in high energy physics experiments; Reseau a multiplexage statistique pour les systemes de selection et de reconstruction d'evenements dans les experiences de physique des hautes energies

Energy Technology Data Exchange (ETDEWEB)

Calvet, D

2000-03-01

Systems for on-line event selection in future high energy physics experiments will use advanced distributed computing techniques and will need high speed networks. After a brief description of projects at the Large Hadron Collider, the architectures initially proposed for the Trigger and Data AcQuisition (TD/DAQ) systems of ATLAS and CMS experiments are presented and analyzed. A new architecture for the ATLAS T/DAQ is introduced. Candidate network technologies for this system are described. This thesis focuses on ATM. A variety of network structures and topologies suited to partial and full event building are investigated. The need for efficient networking is shown. Optimization techniques for high speed messaging and their implementation on ATM components are described. Small scale demonstrator systems consisting of up to 48 computers ({approx}1:20 of the final level 2 trigger) connected via ATM are described. Performance results are presented. Extrapolation of measurements and evaluation of needs lead to a proposal of implementation for the main network of the ATLAS T/DAQ system. (author)

Network based on statistical multiplexing for event selection and event builder systems in high energy physics experiments; Reseau a multiplexage statistique pour les systemes de selection et de reconstruction d'evenements dans les experiences de physique des hautes energies

Energy Technology Data Exchange (ETDEWEB)

Calvet, D

2000-03-01

Systems for on-line event selection in future high energy physics experiments will use advanced distributed computing techniques and will need high speed networks. After a brief description of projects at the Large Hadron Collider, the architectures initially proposed for the Trigger and Data AcQuisition (TD/DAQ) systems of ATLAS and CMS experiments are presented and analyzed. A new architecture for the ATLAS T/DAQ is introduced. Candidate network technologies for this system are described. This thesis focuses on ATM. A variety of network structures and topologies suited to partial and full event building are investigated. The need for efficient networking is shown. Optimization techniques for high speed messaging and their implementation on ATM components are described. Small scale demonstrator systems consisting of up to 48 computers ({approx}1:20 of the final level 2 trigger) connected via ATM are described. Performance results are presented. Extrapolation of measurements and evaluation of needs lead to a proposal of implementation for the main network of the ATLAS T/DAQ system. (author)

Automated transportation management system (ATMS) software project management plan (SPMP)

Energy Technology Data Exchange (ETDEWEB)

Weidert, R.S., Westinghouse Hanford

1996-05-20

The Automated Transportation Management System (ATMS) Software Project Management plan (SPMP) is the lead planning document governing the life cycle of the ATMS and its integration into the Transportation Information Network (TIN). This SPMP defines the project tasks, deliverables, and high level schedules involved in developing the client/server ATMS software.

High speed all-optical networks

Science.gov (United States)

Chlamtac, Imrich

1993-01-01

An inherent problem of conventional point-to-point WAN architectures is that they cannot translate optical transmission bandwidth into comparable user available throughput due to the limiting electronic processing speed of the switching nodes. This report presents the first solution to WDM based WAN networks that overcomes this limitation. The proposed Lightnet architecture takes into account the idiosyncrasies of WDM switching/transmission leading to an efficient and pragmatic solution. The Lightnet architecture trades the ample WDM bandwidth for a reduction in the number of processing stages and a simplification of each switching stage, leading to drastically increased effective network throughputs.

High Speed PAM -8 Optical Interconnects with Digital Equalization based on Neural Network

DEFF Research Database (Denmark)

Gaiarin, Simone; Pang, Xiaodan; Ozolins, Oskars

2016-01-01

We experimentally evaluate a high-speed optical interconnection link with neural network equalization. Enhanced equalization performances are shown comparing to standard linear FFE for an EML-based 32 GBd PAM-8 signal after 4-km SMF transmission.......We experimentally evaluate a high-speed optical interconnection link with neural network equalization. Enhanced equalization performances are shown comparing to standard linear FFE for an EML-based 32 GBd PAM-8 signal after 4-km SMF transmission....

ATM security via "Stargate" solution

OpenAIRE

Hensley, Katrina; Ludden, Fredrick

1999-01-01

Approved for public release, distribution unlimited. In today's world of integrating voice, video and data into a single network, Asynchronous Transfer Mode (ATM) networks have become prevalent in the Department of Defense. The Department of Defense's critical data will have to pass through public networks, which causes concern for security. This study presents an efficient solution aimed at authenticating communications over public ATM networks. The authenticating device, Stargate, utiliz...

Wireless Connectivity to ATM Communication Grid

National Research Council Canada - National Science Library

Rajaravivarma, Veeramuthu

1998-01-01

The AFOSR funds were used to purchase a 12 port Fore ATM switch, ATM network interface cards, a SUN UltraSPARC workstation, Lucent WavePoint wireless bridge, and Lucent WaveLAN wireless network interface cards...

HIPPI-6400 -- Designing for speed

Energy Technology Data Exchange (ETDEWEB)

Tolmie, D.E.

1998-03-01

The emerging High Performance Parallel Interface 6400 Mbit/s interface (HIPPI-6400), is targeted as a local area network (LAN), or system area network (SAN), supporting data rates of 6400 Mbit/s (800 Mbyte/s). This is eight times the speed of Gigabit Ethernet. The features used, and the design choices made, for the data link and physical layers of HIPPI-6400, to achieve this unprecedented speed are the subject of this paper. HIPPI-6400 borrowed freely from other successful technologies such as ATM, Ethernet and the original HIPPI -- taking the best features of each and melding them with some new features. HIPPI-6400 is a cost effective reliable interconnect for distances up to 1 km; it intermixes large and small messages efficiently.

Hierarchy Bayesian model based services awareness of high-speed optical access networks

Science.gov (United States)

Bai, Hui-feng

2018-03-01

As the speed of optical access networks soars with ever increasing multiple services, the service-supporting ability of optical access networks suffers greatly from the shortage of service awareness. Aiming to solve this problem, a hierarchy Bayesian model based services awareness mechanism is proposed for high-speed optical access networks. This approach builds a so-called hierarchy Bayesian model, according to the structure of typical optical access networks. Moreover, the proposed scheme is able to conduct simple services awareness operation in each optical network unit (ONU) and to perform complex services awareness from the whole view of system in optical line terminal (OLT). Simulation results show that the proposed scheme is able to achieve better quality of services (QoS), in terms of packet loss rate and time delay.

High speed fiber optics local area networks: Design and implementation

Science.gov (United States)

Tobagi, Fouad A.

1988-01-01

The design of high speed local area networks (HSLAN) for communication among distributed devices requires solving problems in three areas: (1) the network medium and its topology; (2) the medium access control; and (3) the network interface. Considerable progress has been made in all areas. Accomplishments are divided into two groups according to their theoretical or experimental nature. A brief summary is given in Section 2, including references to papers which appeared in the literature, as well as to Ph.D. dissertations and technical reports published at Stanford University.

Fault-tolerance techniques for high-speed fiber-optic networks

Science.gov (United States)

Deruiter, John

1991-01-01

Four fiber optic network topologies (linear bus, ring, central star, and distributed star) are discussed relative to their application to high data throughput, fault tolerant networks. The topologies are also examined in terms of redundancy and the need to provide for single point, failure free (or better) system operation. Linear bus topology, although traditionally the method of choice for wire systems, presents implementation problems when larger fiber optic systems are considered. Ring topology works well for high speed systems when coupled with a token passing protocol, but it requires a significant increase in protocol complexity to manage system reconfiguration due to ring and node failures. Star topologies offer a natural fault tolerance, without added protocol complexity, while still providing high data throughput capability.

Implementation of virtual LANs over ATM WANs

Science.gov (United States)

Braun, Torsten; Maehler, Martin

1998-09-01

Virtual LANs (VLANs) allow to interconnect users over campus or wide area networks and gives the users the impression as they would be connected to the same local area network (LAN). The implementation of VLANs is based on ATM Forum's LAN Emulation and LAN/ATM switches providing interconnection of emulated LANs over ATM and the LAN ports to which the user's end systems are attached to. The paper discusses possible implementation architectures and describes advanced features such as ATM short-cuts, QoS, and redundancy concepts.

A Control Simulation Method of High-Speed Trains on Railway Network with Irregular Influence

International Nuclear Information System (INIS)

Yang Lixing; Li Xiang; Li Keping

2011-01-01

Based on the discrete time method, an effective movement control model is designed for a group of highspeed trains on a rail network. The purpose of the model is to investigate the specific traffic characteristics of high-speed trains under the interruption of stochastic irregular events. In the model, the high-speed rail traffic system is supposed to be equipped with the moving-block signalling system to guarantee maximum traversing capacity of the railway. To keep the safety of trains' movements, some operational strategies are proposed to control the movements of trains in the model, including traction operation, braking operation, and entering-station operation. The numerical simulations show that the designed model can well describe the movements of high-speed trains on the rail network. The research results can provide the useful information not only for investigating the propagation features of relevant delays under the irregular disturbance but also for rerouting and rescheduling trains on the rail network. (general)

A Managerial Analysis of ATM in Facilitating Distance Education.

Science.gov (United States)

Littman, Marlyn Kemper

In this paper, the fundamental characteristics and capabilities of ATM (Asynchronous Transfer Mode) networks in a distance learning environment are examined. Current and projected ATM applications are described, and issues and challenges associated with developing ATM networking solutions for instructional delivery are explored. Other topics…

Introduction of a super speed network in JNC

International Nuclear Information System (INIS)

Nosaki, Nobuhisa; Aoki, Kazuhisa; Narita, Nobuiku

2002-01-01

The construction of a super speed and broadband network with high cost performance is now possible through the resent remarkable development of information-communication technologies. Under such circumstances, the modification of LAN and WAN to the super speed network was required in accordance with the recent network demand in JNC. The trend of the information-communication technologies and the needs of information-communication infrastructure were reviewed as the first step. A modification of WAN to the super speed network with high cost performance was established in fiscal year 2000. This paper introduces the work procedures and the modification of the WAN in order to acquire a reduction in the running cost and the super speed network in the fiscal year. (author)

A Virtual Private Local PCN Ring Network Based on ATM VP Cross—Connection

Institute of Scientific and Technical Information of China (English)

LinBin; MaYingjun; 等

1995-01-01

Avirtual private local PCNring network (VPLPR)is proposed .VPLPR is a virtual logic ring seuved for digital cordless telephone system and it works on ATM VP cross-connection mechanism.Full-distributed data bases are organized for visitor location registers(VLR)and home location register(HLR).The signaling protocols are compatible upward to B-ISDN. The architecture and some of the main characteristics of VPLPR are given.How to configure the ATM VP cross-connection ring is described.And then a protocol conversion between STM frames and ATMcells in base station controller(BSC)is presented.

Synchronized High-Speed Vision Sensor Network for Expansion of Field of View

Directory of Open Access Journals (Sweden)

Akihito Noda

2018-04-01

Full Text Available We propose a 500-frames-per-second high-speed vision (HSV sensor network that acquires frames at a timing that is precisely synchronized across the network. Multiple vision sensor nodes, individually comprising a camera and a PC, are connected via Ethernet for data transmission and for clock synchronization. A network of synchronized HSV sensors provides a significantly expanded field-of-view compared with that of each individual HSV sensor. In the proposed system, the shutter of each camera is controlled based on the clock of the PC locally provided inside the node, and the shutters are globally synchronized using the Precision Time Protocol (PTP over the network. A theoretical analysis and experiment results indicate that the shutter trigger skew among the nodes is a few tens of microseconds at most, which is significantly smaller than the frame interval of 1000-fps-class high-speed cameras. Experimental results obtained with the proposed system comprising four nodes demonstrated the ability to capture the propagation of a small displacement along a large-scale structure.

System, apparatus and methods to implement high-speed network analyzers

Science.gov (United States)

Ezick, James; Lethin, Richard; Ros-Giralt, Jordi; Szilagyi, Peter; Wohlford, David E

2015-11-10

Systems, apparatus and methods for the implementation of high-speed network analyzers are provided. A set of high-level specifications is used to define the behavior of the network analyzer emitted by a compiler. An optimized inline workflow to process regular expressions is presented without sacrificing the semantic capabilities of the processing engine. An optimized packet dispatcher implements a subset of the functions implemented by the network analyzer, providing a fast and slow path workflow used to accelerate specific processing units. Such dispatcher facility can also be used as a cache of policies, wherein if a policy is found, then packet manipulations associated with the policy can be quickly performed. An optimized method of generating DFA specifications for network signatures is also presented. The method accepts several optimization criteria, such as min-max allocations or optimal allocations based on the probability of occurrence of each signature input bit.

An asynchronous data-driven event-building scheme based on ATM switching fabrics

International Nuclear Information System (INIS)

Letheren, M.; Christiansen, J.; Mandjavidze, I.; Verhille, H.; De Prycker, M.; Pauwels, B.; Petit, G.; Wright, S.; Lumley, J.

1994-01-01

The very high data rates expected in experiments at the next generation of high luminosity hadron colliders will be handled by pipelined front-end readout electronics and multiple levels (2 or 3) of triggering. A variety of data acquisition architectures have been proposed for use downstream of the first level trigger. Depending on the architecture, the aggregate bandwidths required for event building are expected to be of the order 10--100 Gbit/s. Here, an Asynchronous Transfer Mode (ATM) packet-switching network technology is proposed as the interconnect for building high-performance, scalable data acquisition architectures. This paper introduces the relevant characteristics of ATM and describes components for the construction of an ATM-based event builder: (1) a multi-path, self-routing, scalable ATM switching fabric, (2) an experimental high performance workstation ATM-interface, and (3) a VMEbus ATM-interface. The requirement for traffic shaping in ATM-based event-builders is discussed and an analysis of the performance of several such schemes is presented

The Wireless ATM Architecture

Directory of Open Access Journals (Sweden)

R. Palitefka

1998-06-01

Full Text Available An overview of the proposed wireless ATM structure is provided. Wireless communication have been developed to a level where offered services can now be extended beyond voice and data. There are already wireless LANs, cordless systems offering data services and mobile data. Wireless LAN systems are basically planned for local, on-promises and in-house networking providing short distance radio or infrared links between computer system. The main challenge of wireless ATM is to harmonise the development of broadband wireless system with service B -ISDN/ATM and ATM LANs, and offer multimedia multiservice features for the support of time-sensitive voice communication, video, desktop multimedia applications, and LAN data traffic for the wireless user.

Capacity factors of a mixed speed railway network

DEFF Research Database (Denmark)

Harrod, Steven

2009-01-01

Fifty-four combinations of track network and speed differential are evaluated within a linear, discrete time network model that maximizes an objective function of train volume, delays, and idle train time. The results contradict accepted dispatching practice by suggesting that when introducing...... a priority, high-speed train onto a network, maximum network now is attained when the priority train operates at maximum speed. in addition, increasing siding capacity at meeting points may offer a network capacity improvement comparable to partial double track. (C) 2009 Elsevier Ltd. All rights reserved....

ATM supports gammaherpesvirus replication by attenuating type I interferon pathway.

Science.gov (United States)

Darrah, Eric J; Stoltz, Kyle P; Ledwith, Mitchell; Tarakanova, Vera L

2017-10-01

Ataxia-Telangiectasia mutated (ATM) kinase participates in multiple networks, including DNA damage response, oxidative stress, and mitophagy. ATM also supports replication of diverse DNA and RNA viruses. Gammaherpesviruses are prevalent cancer-associated viruses that benefit from ATM expression during replication. This proviral role of ATM had been ascribed to its signaling within the DNA damage response network; other functions of ATM have not been considered. In this study increased type I interferon (IFN) responses were observed in ATM deficient gammaherpesvirus-infected macrophages. Using a mouse model that combines ATM and type I IFN receptor deficiencies we show that increased type I IFN response in the absence of ATM fully accounts for the proviral role of ATM during gammaherpesvirus replication. Further, increased type I IFN response rendered ATM deficient macrophages more susceptible to antiviral effects of type II IFN. This study identifies attenuation of type I IFN responses as the primary mechanism underlying proviral function of ATM during gammaherpesvirus infection. Copyright © 2017 Elsevier Inc. All rights reserved.

A perspective on how ATM lost Control

NARCIS (Netherlands)

Crosby, S.; Rooney, S.; Isaacs, R.; Bos, H.J.

2002-01-01

Contrary to the initial high expectations, ATM failed to become the universal network technology covering all services and running from the desktop to the back-bone. This paper tries to identify the technological problems that contributed to this failure.

Limits to high-speed simulations of spiking neural networks using general-purpose computers.

Science.gov (United States)

Zenke, Friedemann; Gerstner, Wulfram

2014-01-01

To understand how the central nervous system performs computations using recurrent neuronal circuitry, simulations have become an indispensable tool for theoretical neuroscience. To study neuronal circuits and their ability to self-organize, increasing attention has been directed toward synaptic plasticity. In particular spike-timing-dependent plasticity (STDP) creates specific demands for simulations of spiking neural networks. On the one hand a high temporal resolution is required to capture the millisecond timescale of typical STDP windows. On the other hand network simulations have to evolve over hours up to days, to capture the timescale of long-term plasticity. To do this efficiently, fast simulation speed is the crucial ingredient rather than large neuron numbers. Using different medium-sized network models consisting of several thousands of neurons and off-the-shelf hardware, we compare the simulation speed of the simulators: Brian, NEST and Neuron as well as our own simulator Auryn. Our results show that real-time simulations of different plastic network models are possible in parallel simulations in which numerical precision is not a primary concern. Even so, the speed-up margin of parallelism is limited and boosting simulation speeds beyond one tenth of real-time is difficult. By profiling simulation code we show that the run times of typical plastic network simulations encounter a hard boundary. This limit is partly due to latencies in the inter-process communications and thus cannot be overcome by increased parallelism. Overall, these results show that to study plasticity in medium-sized spiking neural networks, adequate simulation tools are readily available which run efficiently on small clusters. However, to run simulations substantially faster than real-time, special hardware is a prerequisite.

Multimedia information processing in the SWAN mobile networked computing system

Science.gov (United States)

Agrawal, Prathima; Hyden, Eoin; Krzyzanowsji, Paul; Srivastava, Mani B.; Trotter, John

1996-03-01

Anytime anywhere wireless access to databases, such as medical and inventory records, can simplify workflow management in a business, and reduce or even eliminate the cost of moving paper documents. Moreover, continual progress in wireless access technology promises to provide per-user bandwidths of the order of a few Mbps, at least in indoor environments. When combined with the emerging high-speed integrated service wired networks, it enables ubiquitous and tetherless access to and processing of multimedia information by mobile users. To leverage on this synergy an indoor wireless network based on room-sized cells and multimedia mobile end-points is being developed at AT&T Bell Laboratories. This research network, called SWAN (Seamless Wireless ATM Networking), allows users carrying multimedia end-points such as PDAs, laptops, and portable multimedia terminals, to seamlessly roam while accessing multimedia data streams from the wired backbone network. A distinguishing feature of the SWAN network is its use of end-to-end ATM connectivity as opposed to the connectionless mobile-IP connectivity used by present day wireless data LANs. This choice allows the wireless resource in a cell to be intelligently allocated amongst various ATM virtual circuits according to their quality of service requirements. But an efficient implementation of ATM in a wireless environment requires a proper mobile network architecture. In particular, the wireless link and medium-access layers need to be cognizant of the ATM traffic, while the ATM layers need to be cognizant of the mobility enabled by the wireless layers. This paper presents an overview of SWAN's network architecture, briefly discusses the issues in making ATM mobile and wireless, and describes initial multimedia applications for SWAN.

Fabrication and characterization of MCC [Materials Characterization Center] approved testing material---ATM-2, ATM-3, and ATM-4 glasses

International Nuclear Information System (INIS)

Wald, J.W.

1988-03-01

Materials Characterization Center glasses ATM-2, ATM-3, and ATM-4 are designed to simulate high-level waste glasses that are likely to result from the reprocessing of commercial nuclear reactor fuels. The three Approved Testing Materials (ATMs) are borosilicate glasses based upon the MCC-76-68 glass composition. One radioisotope was added to form each ATM. The radioisotopes added to form ATM-2, ATM-3, and ATM-4 were 241 Am, 237 Np, and 239 Pu, respectively. Each of the ATM lots was produced in a nominal lot size of 450 g from feed stock melted in a nitrogen-atmosphere glove box at 1200/degree/C in a platinum crucible. Each ATM was then cast into bars. Analyzed compositions of these glasses are listed. The nonradioactive elements were analyzed by inductively coupled argon plasma atomic emission spectroscopy (ICP), and the radioisotope analyses were done by alpha energy analysis. Results are discussed. 7 refs., 3 figs., 5 tabs

Simplified management of ATM traffic

Science.gov (United States)

Luoma, Marko; Ilvesmaeki, Mika

1997-10-01

ATM has been under a thorough standardization process for more than ten years. Looking at it now, what have we achieved during this time period? Originally ATM was meant to be an easy and efficient protocol enabling varying services over a single network. What it is turning to be it `yet another ISDN'--network full of hopes and promises but too difficult to implement and expensive to market. The fact is that more and more `nice features' are implemented on the cost of overloading network with hard management procedures. Therefore we need to adopt a new approach. This approach keeps a strong reminder on `what is necessary.' This paper presents starting points for an alternative approach to the traffic management. We refer to this approach as `the minimum management principle.' Choosing of the suitable service classes for the ATM network is made difficult by the fact that the more services one implements the more management he needs. This is especially true for the variable bit rate connections that are usually treated based on the stochastic models. Stochastic model, at its best, can only reveal momentary characteristics in the traffic stream not the long range behavior of it. Our assumption is that ATM will move towards Internet in the sense that strict values for quality make little or no sense in the future. Therefore stochastic modeling of variable bit rate connections seems to be useless. Nevertheless we see that some traffic needs to have strict guarantees and that the only economic way of doing so is to use PCR allocation.

Hardware demonstration of high-speed networks for satellite applications.

Energy Technology Data Exchange (ETDEWEB)

Donaldson, Jonathon W.; Lee, David S.

2008-09-01

This report documents the implementation results of a hardware demonstration utilizing the Serial RapidIO{trademark} and SpaceWire protocols that was funded by Sandia National Laboratories (SNL's) Laboratory Directed Research and Development (LDRD) office. This demonstration was one of the activities in the Modeling and Design of High-Speed Networks for Satellite Applications LDRD. This effort has demonstrated the transport of application layer packets across both RapidIO and SpaceWire networks to a common downlink destination using small topologies comprised of commercial-off-the-shelf and custom devices. The RapidFET and NEX-SRIO debug and verification tools were instrumental in the successful implementation of the RapidIO hardware demonstration. The SpaceWire hardware demonstration successfully demonstrated the transfer and routing of application data packets between multiple nodes and also was able reprogram remote nodes using configuration bitfiles transmitted over the network, a key feature proposed in node-based architectures (NBAs). Although a much larger network (at least 18 to 27 nodes) would be required to fully verify the design for use in a real-world application, this demonstration has shown that both RapidIO and SpaceWire are capable of routing application packets across a network to a common downlink node, illustrating their potential use in real-world NBAs.

Enhancing the Current Automated Teller Machine (ATM) in Nigerian ...

African Journals Online (AJOL)

This is going to be achieved by creating another input device that collects the money into the ATM system, reads its denomination and either saves it or transfers it the required customer ... Keywords: Automated Teller Machine (ATM), Interswitch, Local Area Network (LAN), Wide Area Network (WAN), Telecommunication.

Trend on High-speed Power Line Communication Technology

Science.gov (United States)

Ogawa, Osamu

High-speed power line communication (PLC) is useful technology to easily build the communication networks, because construction of new infrastructure is not necessary. In Europe and America, PLC has been used for broadband networks since the beginning of 21th century. In Japan, high-speed PLC was deregulated only indoor usage in 2006. Afterward it has been widely used for home area network, LAN in hotels and school buildings and so on. And recently, PLC is greatly concerned as communication technology for smart grid network. In this paper, the author surveys the high-speed PLC technology and its current status.

Connecting Remote Clusters with ATM

Energy Technology Data Exchange (ETDEWEB)

Hu, T.C.; Wyckoff, P.S.

1998-10-01

Sandia's entry into utilizing clusters of networked workstations is called Computational Plant or CPlant for short. The design of CPlant uses Ethernet to boot the individual nodes, Myrinet to communicate within a node cluster, and ATM to connect between remote clusters. This SAND document covers the work done to enable the use of ATM on the CPlant nodes in the Fall of 1997.

A Study of Quality of Service Communication for High-Speed Packet-Switching Computer Sub-Networks

Science.gov (United States)

Cui, Zhenqian

1999-01-01

In this thesis, we analyze various factors that affect quality of service (QoS) communication in high-speed, packet-switching sub-networks. We hypothesize that sub-network-wide bandwidth reservation and guaranteed CPU processing power at endpoint systems for handling data traffic are indispensable to achieving hard end-to-end quality of service. Different bandwidth reservation strategies, traffic characterization schemes, and scheduling algorithms affect the network resources and CPU usage as well as the extent that QoS can be achieved. In order to analyze those factors, we design and implement a communication layer. Our experimental analysis supports our research hypothesis. The Resource ReSerVation Protocol (RSVP) is designed to realize resource reservation. Our analysis of RSVP shows that using RSVP solely is insufficient to provide hard end-to-end quality of service in a high-speed sub-network. Analysis of the IEEE 802.lp protocol also supports the research hypothesis.

Adaptive Network Coded Clouds: High Speed Downloads and Cost-Effective Version Control

DEFF Research Database (Denmark)

Sipos, Marton A.; Heide, Janus; Roetter, Daniel Enrique Lucani

2018-01-01

Although cloud systems provide a reliable and flexible storage solution, the use of a single cloud service constitutes a single point of failure, which can compromise data availability, download speed, and security. To address these challenges, we advocate for the use of multiple cloud storage...... providers simultaneously using network coding as the key enabling technology. Our goal is to study two challenges of network coded storage systems. First, the efficient update of the number of coded fragments per cloud in a system aggregating multiple clouds in order to boost the download speed of files. We...... developed a novel scheme using recoding with limited packets to trade-off storage space, reliability, and data retrieval speed. Implementation and measurements with commercial cloud providers show that up to 9x less network use is needed compared to other network coding schemes, while maintaining similar...

Enhanced ATM Security using Biometric Authentication and Wavelet Based AES

Directory of Open Access Journals (Sweden)

Sreedharan Ajish

2016-01-01

Full Text Available The traditional ATM terminal customer recognition systems rely only on bank cards, passwords and such identity verification methods are not perfect and functions are too single. Biometrics-based authentication offers several advantages over other authentication methods, there has been a significant surge in the use of biometrics for user authentication in recent years. This paper presents a highly secured ATM banking system using biometric authentication and wavelet based Advanced Encryption Standard (AES algorithm. Two levels of security are provided in this proposed design. Firstly we consider the security level at the client side by providing biometric authentication scheme along with a password of 4-digit long. Biometric authentication is achieved by considering the fingerprint image of the client. Secondly we ensure a secured communication link between the client machine to the bank server using an optimized energy efficient and wavelet based AES processor. The fingerprint image is the data for encryption process and 4-digit long password is the symmetric key for the encryption process. The performance of ATM machine depends on ultra-high-speed encryption, very low power consumption, and algorithmic integrity. To get a low power consuming and ultra-high speed encryption at the ATM machine, an optimized and wavelet based AES algorithm is proposed. In this system biometric and cryptography techniques are used together for personal identity authentication to improve the security level. The design of the wavelet based AES processor is simulated and the design of the energy efficient AES processor is simulated in Quartus-II software. Simulation results ensure its proper functionality. A comparison among other research works proves its superiority.

The ATM signaling network in development and disease

Science.gov (United States)

Stracker, Travis H.; Roig, Ignasi; Knobel, Philip A.; Marjanović, Marko

2013-01-01

The DNA damage response (DDR) rapidly recognizes DNA lesions and initiates the appropriate cellular programs to maintain genome integrity. This includes the coordination of cell cycle checkpoints, transcription, translation, DNA repair, metabolism, and cell fate decisions, such as apoptosis or senescence (Jackson and Bartek, 2009). DNA double-strand breaks (DSBs) represent one of the most cytotoxic DNA lesions and defects in their metabolism underlie many human hereditary diseases characterized by genomic instability (Stracker and Petrini, 2011; McKinnon, 2012). Patients with hereditary defects in the DDR display defects in development, particularly affecting the central nervous system, the immune system and the germline, as well as aberrant metabolic regulation and cancer predisposition. Central to the DDR to DSBs is the ataxia-telangiectasia mutated (ATM) kinase, a master controller of signal transduction. Understanding how ATM signaling regulates various aspects of the DDR and its roles in vivo is critical for our understanding of human disease, its diagnosis and its treatment. This review will describe the general roles of ATM signaling and highlight some recent advances that have shed light on the diverse roles of ATM and related proteins in human disease. PMID:23532176

The ATM signaling network in development and disease

Directory of Open Access Journals (Sweden)

Travis H. Stracker

2013-03-01

Full Text Available The DNA damage response (DDR rapidly recognizes DNA lesions and initiates the appropriate cellular programs to maintain genome integrity. This includes the coordination of cell cycle checkpoints, transcription, translation, DNA repair, metabolism and cell fate decisions, such as apoptosis or senescence(Jackson and Bartek, 2009. DNA double-strand breaks (DSBs represent one of the most cytotoxic DNA lesions and defects in their metabolism underlie many human hereditary diseases characterized by genomic instability(Stracker and Petrini, 2011;McKinnon, 2012. Patients with hereditary defects in the DDR display defects in development, particularly affecting the central nervous system (CNS, the immune system and the germline, as well as aberrant metabolic regulation and cancer predisposition. Central to the DDR to DSBs is the ATM kinase, a master controller of signal transduction. Understanding how ATM signaling regulates various aspects of the DDR and its roles in vivo is critical for our understanding of human disease, its diagnosis and its treatment. This review will describe the general roles of ATM signaling and highlight some recent advances that have shed light on the diverse roles of ATM and related proteins in human disease.

Gigabit ATM: another technical mistake?

Science.gov (United States)

Christ, Paul

1998-09-01

Once upon a time, or more precisely during February 1988 at the CCITT Seoul plenary, and definitely arriving as a revolution, ATM hit the hard-core B-ISDN circuit-switching gang. Initiated by the Telecoms' camp, but, surprisingly, soon to be pushed by computer minded people, ATM's generic technological history is somewhat richer than single-sided stories. Here are two classical elements of that history: Firstly, together with X.25, ATM suffers from the connection versus datagram dichotomy, well known for more than twenty years. Secondly, and lesser known, ATM's use of cells in support of the 'I' of B-ISDN was questioned from the very beginning by the packet switching camp. Furthermore, in this context, there are two other essential elements to be considered: Firstly, the exponential growth of the Internet and later intranets, using Internet technology, sparked by the success of the Web and the WINTEL alliance, resulted in a corresponding demand for both aggregate and end-system network bandwidth. Secondly, servers, historically restricted to the exclusive club of HIPPI-equipped supercomputers, suddenly become ordinary high-end PCs with 64-bit wide PCI busses -- definitely aiming at the Gigabit. Here, if your aim is for Gigabit ATM with 5000-transactions per second classical supercomputers, a 65K ATM MTU -- as implemented by Cray -- might be okay. Following Clark and others, another part of the story is the adoption and redefinition, by the IETF, of the Telecoms' notion of 'Integrated Services' and QoS mechanisms. The quest for low-delay IP packet forwarding, perhaps possible over ATM cut-throughs, has resulted in the switching versus/or integrated-with-routing movement. However, a blow for ATM may be the recent results concerning fast routing table lookup algorithms. This, by making Gigabit routing possible using ordinary Pentium processors may eventually render the much prophesized ATM switching performance unnecessary. Recently, with the rise of Gigabit Ethernet

ATM LAN Emulation: Getting from Here to There.

Science.gov (United States)

Learn, Larry L., Ed.

1995-01-01

Discusses current LAN (local area network) configuration and explains ATM (asynchronous transfer mode) as the future telecommunications transport. Highlights include LAN emulation, which enables the interconnection of legacy LANs and the new ATM environment; virtual LANs; broadcast servers; and standards. (LRW)

Cognitive radio networks efficient resource allocation in cooperative sensing, cellular communications, high-speed vehicles, and smart grid

CERN Document Server

Jiang, Tao; Cao, Yang

2015-01-01

PrefaceAcknowledgmentsAbout the AuthorsIntroductionCognitive Radio-Based NetworksOpportunistic Spectrum Access NetworksCognitive Radio Networks with Cooperative SensingCognitive Radio Networks for Cellular CommunicationsCognitive Radio Networks for High-Speed VehiclesCognitive Radio Networks for a Smart GridContent and OrganizationTransmission Slot Allocation in an Opportunistic Spectrum Access NetworkSingle-User Single-Channel System ModelProbabilistic Slot Allocation SchemeOptimal Probabilistic Slot AllocationBaseline PerformanceExponential DistributionHyper-Erlang DistributionPerformance An

Optimization of wide-area ATM and local-area ethernet/FDDI network configurations for high-speed telemedicine communications employing NASA's ACTS.

Science.gov (United States)

McDermott, W R; Tri, J L; Mitchell, M P; Levens, S P; Wondrow, M A; Huie, L M; Khandheria, B K; Gilbert, B K

1999-01-01

A high data rate terrestrial and satellite network was implemented to transfer medical images and data. This article describes the a optimization of the workstations and switching equipment incorporated into the network. Topics discussed in this article include tuning of the network software, the configuration of the Sun Microsystems workstations, the FORE Systems asynchronous transfer mode switches, as well as the throughput results of two telemedicine experiments undertaken by Mayo's physician staff. The technical staff was successful in achieving the data throughput needed by the telemedicine software; particularly important was the proper determination of peak throughput and TCP window sizes to ensure optimum use of the resources available on the Sun Microsystems and Hewlett Packard workstations.

Rad-Tolerant, Thermally Stable, High-Speed Fiber-Optic Network for Harsh Environments

Science.gov (United States)

Leftwich, Matt; Hull, Tony; Leary, Michael; Leftwich, Marcus

2013-01-01

Future NASA destinations will be challenging to get to, have extreme environmental conditions, and may present difficulty in retrieving a spacecraft or its data. Space Photonics is developing a radiation-tolerant (rad-tolerant), high-speed, multi-channel fiber-optic transceiver, associated reconfigurable intelligent node communications architecture, and supporting hardware for intravehicular and ground-based optical networking applications. Data rates approaching 3.2 Gbps per channel will be achieved.

DNA Damage-Induced Acetylation of Lysine 3016 of ATM Activates ATM Kinase Activity▿ †

OpenAIRE

Sun, Yingli; Xu, Ye; Roy, Kanaklata; Price, Brendan D.

2007-01-01

The ATM protein kinase is essential for cells to repair and survive genotoxic events. The activation of ATM's kinase activity involves acetylation of ATM by the Tip60 histone acetyltransferase. In this study, systematic mutagenesis of lysine residues was used to identify regulatory ATM acetylation sites. The results identify a single acetylation site at lysine 3016, which is located in the highly conserved C-terminal FATC domain adjacent to the kinase domain. Antibodies specific for acetyl-ly...

Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS).

Science.gov (United States)

Enari, Masato; Matsushima-Hibiya, Yuko; Miyazaki, Makoto; Otomo, Ryo

2017-01-01

Ataxia-telangiectasia mutated (ATM) protein is a member of the phosphatidylinositol 3-phosphate kinase (PI3-K)-related protein kinase (PIKK) family and is implicated in the initiation of signaling pathways following DNA double strand breaks (DSBs) elicited by exposure to ionizing irradiation (IR) or radiomimetic compounds. Loss of function of the ATM gene product results in the human genetic disorder ataxia-telangiectasia (A-T) characterized by neurodegeneration, immunodeficiency, genomic instability, and cancer predisposition. In response to DSBs, ATM is activated and phosphorylates Ser/Thr-Gln (S/T-Q) sequences on numerous proteins participating in DNA-damage responses. Among these proteins, phosphorylation of the tumor suppressor p53 at Ser15 is known as a target for ATM, which leads to the dissociation of MDM2, an E3 ubiquitin ligase, from p53 to prevent MDM2-dependent p53 degradation. Ser46 on p53 is phosphorylated in response to DSBs and contributes to the preferential transactivation of pro-apoptotic genes, such as p53AIP1, Noxa, and PUMA, to prevent tumor formation. Our group have shown that not only ATM preferentially phosphorylates S/T-Q sequences, but also Ser46, which is a noncanonical site with an S-P sequence for ATM. Ser46 on p53 is directly phosphorylated by ATM in a p53 conformation-dependent manner using the ATP analogue-accepting ATM mutant (ATM-AS) system. This protocol summarizes an approach to identify direct numerous targets for ATM kinase and is used to elucidate ATM signaling pathways in the DNA damage responses.

High-speed railway real-time localization auxiliary method based on deep neural network

Science.gov (United States)

Chen, Dongjie; Zhang, Wensheng; Yang, Yang

2017-11-01

High-speed railway intelligent monitoring and management system is composed of schedule integration, geographic information, location services, and data mining technology for integration of time and space data. Assistant localization is a significant submodule of the intelligent monitoring system. In practical application, the general access is to capture the image sequences of the components by using a high-definition camera, digital image processing technique and target detection, tracking and even behavior analysis method. In this paper, we present an end-to-end character recognition method based on a deep CNN network called YOLO-toc for high-speed railway pillar plate number. Different from other deep CNNs, YOLO-toc is an end-to-end multi-target detection framework, furthermore, it exhibits a state-of-art performance on real-time detection with a nearly 50fps achieved on GPU (GTX960). Finally, we realize a real-time but high-accuracy pillar plate number recognition system and integrate natural scene OCR into a dedicated classification YOLO-toc model.

High-speed quantum networking by ship

Science.gov (United States)

Devitt, Simon J.; Greentree, Andrew D.; Stephens, Ashley M.; van Meter, Rodney

2016-11-01

Networked entanglement is an essential component for a plethora of quantum computation and communication protocols. Direct transmission of quantum signals over long distances is prevented by fibre attenuation and the no-cloning theorem, motivating the development of quantum repeaters, designed to purify entanglement, extending its range. Quantum repeaters have been demonstrated over short distances, but error-corrected, global repeater networks with high bandwidth require new technology. Here we show that error corrected quantum memories installed in cargo containers and carried by ship can provide a exible connection between local networks, enabling low-latency, high-fidelity quantum communication across global distances at higher bandwidths than previously proposed. With demonstrations of technology with sufficient fidelity to enable topological error-correction, implementation of the quantum memories is within reach, and bandwidth increases with improvements in fabrication. Our approach to quantum networking avoids technological restrictions of repeater deployment, providing an alternate path to a worldwide Quantum Internet.

Wireless Interconnect in Multilayer Chip-Area-Networks for Future Multimaterial High-Speed Systems Design

Directory of Open Access Journals (Sweden)

Oluwole John Famoriji

2017-01-01

Full Text Available Wireless chip area network which enables wireless communication among chips fosters development in wireless communication and it is envisioned that future hardware system and developmental functionality will require multimaterial. However, the traditional system architecture is limited by channel bandwidth-limited interfaces, throughput, delay, and power consumption and as a result limits the efficiency and system performance. Wireless interconnect has been proposed to overcome scalability and performance limitations of multihop wired architectures. Characterization and modeling of channel become more important for specification of choice of modulation or demodulation techniques, channel bandwidths, and other mitigation techniques for channel distortion and interference such as equalization. This paper presents an analytical channel model for characterization, modeling, and analysis of wireless chip-to-chip or interchip interconnects in wireless chip area network with a particular focus on large-scale analysis. The proposed model accounts for both static and dynamic channel losses/attenuation in high-speed systems. Simulation and evaluation of the model with experimental data conducted in a computer desktop casing depict that proposed model matched measurement data very closely. The transmission of EM waves via a medium introduces molecular absorption due to various molecules within the material substance. This model is a representative of channel loss profile in wireless chip-area-network communication and good for future electronic circuits and high-speed systems design.

Performance analysis of ATM/DQDB interworking

DEFF Research Database (Denmark)

Christiansen, Henning; Kvols, Kenn

1992-01-01

The cell loss ratio and cell delay variation of a distributed-queue dual-bus (DQDB) network receiving traffic from a number of asynchronous transfer mode (ATM) connections are considered. Every connection carries either connection oriented or connectionless traffic. In the analysis of the access ...... to the bus, it is shown that consecutive service times of the local access queue are correlated. Two models, one of which includes the correlation, are presented. The correlation effect is illustrated and the models are evaluated by means of a number of simulation cases......The cell loss ratio and cell delay variation of a distributed-queue dual-bus (DQDB) network receiving traffic from a number of asynchronous transfer mode (ATM) connections are considered. Every connection carries either connection oriented or connectionless traffic. In the analysis of the access...

Introduction to multiprotocol over ATM (MPOA)

Science.gov (United States)

Fredette, Andre N.

1997-10-01

Multiprotocol over ATM (MPOA) is a new protocol specified by the ATM Forum. MPOA provides a framework for effectively synthesizing bridging and routing with ATM in an environment of diverse protocols and network technologies. The primary goal of MPOA is the efficient transfer of inter-subnet unicast data in a LAN Emulation (LANE) environment. MPOA integrates LANE and the next hop resolution protocol (NHRP) to preserve the benefits of LAN Emulation, while allowing inter-subnet, internetwork layer protocol communication over ATM VCCs without requiring routers in the data path. It reduces latency and the internetwork layer forwarding load on backbone routers by enabling direct connectivity between ATM-attached edge devices (i.e., shortcuts). To establish these shortcuts, MPOA uses both routing and bridging information to locate the edge device closest to the addressed end station. By integrating LANE and NHRP, MPOA allows the physical separation of internetwork layer route calculation and forwarding, a technique known as virtual routing. This separation provides a number of key benefits including enhanced manageability and reduced complexity of internetwork layer capable edge devices. This paper provides an overview of MPOA that summarizes the goals, architecture, and key attributes of the protocol. In presenting this overview, the salient attributes of LANE and NHRP are described as well.

Wireless ATM : handover issues

OpenAIRE

Jiang, Fan; Käkölä, Timo

1998-01-01

Basic aspects of cellular systems and the ATM transmission technology are introduced. Wireless ATM is presented as a combination of radio ATM and mobile ATM. Radio ATM is a wireless extension of an ATM connection while mobile ATM contains the necessary extensions to ATM to support mobility. Because the current ATM technology does not support mobility, handover becomes one of the most important research issues for wireless ATM. Wireless ATM handover requirements are thus analysed. A handover s...

Performance of asynchronous transfer mode (ATM) local area and wide area networks for medical imaging transmission in clinical environment.

Science.gov (United States)

Huang, H K; Wong, A W; Zhu, X

1997-01-01

Asynchronous transfer mode (ATM) technology emerges as a leading candidate for medical image transmission in both local area network (LAN) and wide area network (WAN) applications. This paper describes the performance of an ATM LAN and WAN network at the University of California, San Francisco. The measurements were obtained using an intensive care unit (ICU) server connecting to four image workstations (WS) at four different locations of a hospital-integrated picture archiving and communication system (HI-PACS) in a daily regular clinical environment. Four types of performance were evaluated: magnetic disk-to-disk, disk-to-redundant array of inexpensive disks (RAID), RAID-to-memory, and memory-to-memory. Results demonstrate that the transmission rate between two workstations can reach 5-6 Mbytes/s from RAID-to-memory, and 8-10 Mbytes/s from memory-to-memory. When the server has to send images to all four workstations simultaneously, the transmission rate to each WS is about 4 Mbytes/s. Both situations are adequate for radiologic image communications for picture archiving and communication systems (PACS) and teleradiology applications.

Ataxia-telangiectasia gene (ATM) mutation heterozygosity in breast cancer: a narrative review.

Science.gov (United States)

Jerzak, K J; Mancuso, T; Eisen, A

2018-04-01

Despite the fact that heterozygosity for a pathogenic ATM variant is present in 1%-2% of the adult population, clinical guidelines to inform physicians and genetic counsellors about optimal management in that population are lacking. In this narrative review, we describe the challenges and controversies in the management of women who are heterozygous for a pathogenic ATM variant with respect to screening for breast and other malignancies, to choices for systemic therapy, and to decisions about radiation therapy. Given that the lifetime risk for breast cancer in women who are heterozygous for a pathogenic ATM variant is likely greater than 25%, those women should undergo annual mammographic screening starting at least by 40 years of age. For women in this group who have a strong family history of breast cancer, earlier screening with both magnetic resonance imaging and mammography should be considered. High-quality data to inform the management of established breast cancer in carriers of pathogenic ATM variants are lacking. Although deficiency in the ATM gene product might confer sensitivity to dna-damaging pharmaceuticals such as inhibitors of poly (adp-ribose) polymerase or platinum agents, prospective clinical trials have not been conducted in the relevant patient population. Furthermore, the evidence with respect to radiation therapy is mixed; some data suggest increased toxicity, and other data suggest improved clinical benefit from radiation in women who are carriers of a pathogenic ATM variant. As in the 2017 U.S. National Comprehensive Cancer Network guidelines, we recommend high-risk imaging for women in Ontario who are heterozygous for a pathogenic ATM variant. Currently, ATM carrier status should not influence decisions about systemic or radiation therapy in the setting of an established breast cancer diagnosis.

Train-Network Interactions and Stability Evaluation in High-Speed Railways--Part II: Influential Factors and Verifications

DEFF Research Database (Denmark)

Hu, Haitao; Tao, Haidong; Wang, Xiongfei

2018-01-01

Low-frequency oscillation (LFO), harmonic resonance and resonance instability phenomena happened in high speed railways (HSRs) are resulted from the interactions between multiple electric trains and traction network. A train-network interaction system and a unified impedance-based model......, catenary lines and autotransformers (ATs); 3) different numbers and positions of trains and railway lines will also be considered and discussed. In order to validate the theoretical results, the time-domain simulation and experiment system have been conducted. Finally, the differences and the relations...

Location accuracy evaluation of lightning location systems using natural lightning flashes recorded by a network of high-speed cameras

Science.gov (United States)

Alves, J.; Saraiva, A. C. V.; Campos, L. Z. D. S.; Pinto, O., Jr.; Antunes, L.

2014-12-01

This work presents a method for the evaluation of location accuracy of all Lightning Location System (LLS) in operation in southeastern Brazil, using natural cloud-to-ground (CG) lightning flashes. This can be done through a multiple high-speed cameras network (RAMMER network) installed in the Paraiba Valley region - SP - Brazil. The RAMMER network (Automated Multi-camera Network for Monitoring and Study of Lightning) is composed by four high-speed cameras operating at 2,500 frames per second. Three stationary black-and-white (B&W) cameras were situated in the cities of São José dos Campos and Caçapava. A fourth color camera was mobile (installed in a car), but operated in a fixed location during the observation period, within the city of São José dos Campos. The average distance among cameras was 13 kilometers. Each RAMMER sensor position was determined so that the network can observe the same lightning flash from different angles and all recorded videos were GPS (Global Position System) time stamped, allowing comparisons of events between cameras and the LLS. The RAMMER sensor is basically composed by a computer, a Phantom high-speed camera version 9.1 and a GPS unit. The lightning cases analyzed in the present work were observed by at least two cameras, their position was visually triangulated and the results compared with BrasilDAT network, during the summer seasons of 2011/2012 and 2012/2013. The visual triangulation method is presented in details. The calibration procedure showed an accuracy of 9 meters between the accurate GPS position of the object triangulated and the result from the visual triangulation method. Lightning return stroke positions, estimated with the visual triangulation method, were compared with LLS locations. Differences between solutions were not greater than 1.8 km.

Mechanisms of increased risk of tumorigenesis in Atm and Brca1 double heterozygosity

Directory of Open Access Journals (Sweden)

Wang Jufang

2011-08-01

Full Text Available Abstract Background Both epidemiological and experimental studies suggest that heterozygosity for a single gene is linked with tumorigenesis and heterozygosity for two genes increases the risk of tumor incidence. Our previous work has demonstrated that Atm/Brca1 double heterozygosity leads to higher cell transformation rate than single heterozygosity. However, the underlying mechanisms have not been fully understood yet. In the present study, a series of pathways were investigated to clarify the possible mechanisms of increased risk of tumorigenesis in Atm and Brca1 heterozygosity. Methods Wild type cells, Atm or Brca1 single heterozygous cells, and Atm/Brca1 double heterozygous cells were used to investigate DNA damage and repair, cell cycle, micronuclei, and cell transformation after photon irradiation. Results Remarkable high transformation frequency was confirmed in Atm/Brca1 double heterozygous cells compared to wild type cells. It was observed that delayed DNA damage recognition, disturbed cell cycle checkpoint, incomplete DNA repair, and increased genomic instability were involved in the biological networks. Haploinsufficiency of either ATM or BRCA1 negatively impacts these pathways. Conclusions The quantity of critical proteins such as ATM and BRCA1 plays an important role in determination of the fate of cells exposed to ionizing radiation and double heterozygosity increases the risk of tumorigenesis. These findings also benefit understanding of the individual susceptibility to tumor initiation.

Mechanisms of increased risk of tumorigenesis in Atm and Brca1 double heterozygosity

International Nuclear Information System (INIS)

Wang, Jufang; Su, Fengtao; Smilenov, Lubomir B; Zhou, Libin; Hu, Wentao; Ding, Nan; Zhou, Guangming

2011-01-01

Both epidemiological and experimental studies suggest that heterozygosity for a single gene is linked with tumorigenesis and heterozygosity for two genes increases the risk of tumor incidence. Our previous work has demonstrated that Atm/Brca1 double heterozygosity leads to higher cell transformation rate than single heterozygosity. However, the underlying mechanisms have not been fully understood yet. In the present study, a series of pathways were investigated to clarify the possible mechanisms of increased risk of tumorigenesis in Atm and Brca1 heterozygosity. Wild type cells, Atm or Brca1 single heterozygous cells, and Atm/Brca1 double heterozygous cells were used to investigate DNA damage and repair, cell cycle, micronuclei, and cell transformation after photon irradiation. Remarkable high transformation frequency was confirmed in Atm/Brca1 double heterozygous cells compared to wild type cells. It was observed that delayed DNA damage recognition, disturbed cell cycle checkpoint, incomplete DNA repair, and increased genomic instability were involved in the biological networks. Haploinsufficiency of either ATM or BRCA1 negatively impacts these pathways. The quantity of critical proteins such as ATM and BRCA1 plays an important role in determination of the fate of cells exposed to ionizing radiation and double heterozygosity increases the risk of tumorigenesis. These findings also benefit understanding of the individual susceptibility to tumor initiation

ATM QoS Experiments Using TCP Applications: Performance of TCP/IP Over ATM in a Variety of Errored Links

Science.gov (United States)

Frantz, Brian D.; Ivancic, William D.

2001-01-01

Asynchronous Transfer Mode (ATM) Quality of Service (QoS) experiments using the Transmission Control Protocol/Internet Protocol (TCP/IP) were performed for various link delays. The link delay was set to emulate a Wide Area Network (WAN) and a Satellite Link. The purpose of these experiments was to evaluate the ATM QoS requirements for applications that utilize advance TCP/IP protocols implemented with large windows and Selective ACKnowledgements (SACK). The effects of cell error, cell loss, and random bit errors on throughput were reported. The detailed test plan and test results are presented herein.

Regulation of ATM induction

International Nuclear Information System (INIS)

Clarke, R.A.; Fang, Z.M.; Kearsley, J.H.; Lee, C.S.; Sarris, M.; De Murrell, D.

2000-01-01

Full text: ATM, the tumour suppressor protein mutated in ataxia-telangiectasia, is of pivotal importance in controlling the cells primary response to ionising radiation (IR) induced DNA damage. Mutations in ATM which reduce the level of the ATM protein and/or compromise ATM functions are known to give rise to radiosensitivity and defective cell cycle checkpoint control. In response to DNA damage ATM kinase is rapidly activated and initiates downstream signalling to cell cycle control molecules including p53. To investigate additional mechanisms of ATM control we have employed ATM antisense expression in cultured cells, western analyses and immunohistochemistry in situ. We report that ATM can be up-regulated up to 10-fold following exposure to low levels of ionising radiation. ATM radiation-induction was radiation dose dependent while the rapidity of the response indicates a post translational pathway. The concurrent time frames for the radiation-induction of ATM levels and the activation of ATM kinase activity appear to be complimentary in boosting ATM's protective response to IR induced DNA damage, especially in ATM 'low expressing' systems. We also provide the first report of ATM misregulation in 2 cancer patients, indicating that ATM is not only radio-protective but has possible implications in cancer, particularly breast cancer. These results have particular importance in defining the regulation of the ATM protein as an: adaptive radio-response; radio-prognostic market in tumours and normal tissue, and breast cancer marker

Atm reactivation reverses ataxia telangiectasia phenotypes in vivo.

Science.gov (United States)

Di Siena, Sara; Campolo, Federica; Gimmelli, Roberto; Di Pietro, Chiara; Marazziti, Daniela; Dolci, Susanna; Lenzi, Andrea; Nussenzweig, Andre; Pellegrini, Manuela

2018-02-22

Hereditary deficiencies in DNA damage signaling are invariably associated with cancer predisposition, immunodeficiency, radiation sensitivity, gonadal abnormalities, premature aging, and tissue degeneration. ATM kinase has been established as a central player in DNA double-strand break repair and its deficiency causes ataxia telangiectasia, a rare, multi-system disease with no cure. So ATM represents a highly attractive target for the development of novel types of gene therapy or transplantation strategies. Atm tamoxifen-inducible mouse models were generated to explore whether Atm reconstitution is able to restore Atm function in an Atm-deficient background. Body weight, immunodeficiency, spermatogenesis, and radioresistance were recovered in transgenic mice within 1 month from Atm induction. Notably, life span was doubled after Atm restoration, mice were protected from thymoma and no cerebellar defects were observed. Atm signaling was functional after DNA damage in vivo and in vitro. In summary, we propose a new Atm mouse model to investigate novel therapeutic strategies for ATM activation in ataxia telangiectasia disease.

Speed Optimization in Liner Shipping Network Design

DEFF Research Database (Denmark)

Brouer, Berit Dangaard; Karsten, Christian Vad; Pisinger, David

In the Liner Shipping Network Design Problem (LSNDP) services sail at a given speed throughout a round trip. In reality most services operate with a speed differentiated head- and back-haul, or even individual speeds on every sailing between two ports. The speed of a service is decisive...

Scalable Active Optical Access Network Using Variable High-Speed PLZT Optical Switch/Splitter

Science.gov (United States)

Ashizawa, Kunitaka; Sato, Takehiro; Tokuhashi, Kazumasa; Ishii, Daisuke; Okamoto, Satoru; Yamanaka, Naoaki; Oki, Eiji

This paper proposes a scalable active optical access network using high-speed Plumbum Lanthanum Zirconate Titanate (PLZT) optical switch/splitter. The Active Optical Network, called ActiON, using PLZT switching technology has been presented to increase the number of subscribers and the maximum transmission distance, compared to the Passive Optical Network (PON). ActiON supports the multicast slot allocation realized by running the PLZT switch elements in the splitter mode, which forces the switch to behave as an optical splitter. However, the previous ActiON creates a tradeoff between the network scalability and the power loss experienced by the optical signal to each user. It does not use the optical power efficiently because the optical power is simply divided into 0.5 to 0.5 without considering transmission distance from OLT to each ONU. The proposed network adopts PLZT switch elements in the variable splitter mode, which controls the split ratio of the optical power considering the transmission distance from OLT to each ONU, in addition to PLZT switch elements in existing two modes, the switching mode and the splitter mode. The proposed network introduces the flexible multicast slot allocation according to the transmission distance from OLT to each user and the number of required users using three modes, while keeping the advantages of ActiON, which are to support scalable and secure access services. Numerical results show that the proposed network dramatically reduces the required number of slots and supports high bandwidth efficiency services and extends the coverage of access network, compared to the previous ActiON, and the required computation time for selecting multicast users is less than 30msec, which is acceptable for on-demand broadcast services.

THESEUS: A wavelength division multiplexed/microwave subcarrier multiplexed optical network, its ATM switch applications and device requirements

Science.gov (United States)

Xin, Wei

1997-10-01

A Terabit Hybrid Electro-optical /underline[Se]lf- routing Ultrafast Switch (THESEUS) has been proposed. It is a self-routing wavelength division multiplexed (WDM) / microwave subcarrier multiplexed (SCM) asynchronous transfer mode (ATM) switch for the multirate ATM networks. It has potential to be extended to a large ATM switch as 1000 x 1000 without internal blocking. Among the advantages of the hybrid implementation are flexibility in service upgrade, relaxed tolerances on optical filtering, protocol simplification and less processing overhead. For a small ATM switch, the subcarrier can be used as output buffers to solve output contention. A mathematical analysis was conducted to evaluate different buffer configurations. A testbed has been successfully constructed. Multirate binary data streams have been switched through the testbed and error free reception ([<]10-9 bit error rate) has been achieved. A simple, intuitive theoretical model has been developed to describe the heterodyne optical beat interference. A new concept of interference time and interference length has been introduced. An experimental confirmation has been conducted. The experimental results match the model very well. It shows that a large portion of optical bandwidth is wasted due to the beat interference. Based on the model, several improvement approaches have been proposed. The photo-generated carrier lifetime of silicon germanium has been measured using time-resolved reflectivity measurement. Via oxygen ion implantation, the carrier lifetime has been reduced to as short as 1 ps, corresponding to 1 THz of photodetector bandwidth. It has also been shown that copper dopants act as recombination centers in the silicon germanium.

High-Speed Rail Train Timetabling Problem: A Time-Space Network Based Method with an Improved Branch-and-Price Algorithm

Directory of Open Access Journals (Sweden)

Bisheng He

2014-01-01

Full Text Available A time-space network based optimization method is designed for high-speed rail train timetabling problem to improve the service level of the high-speed rail. The general time-space path cost is presented which considers both the train travel time and the high-speed rail operation requirements: (1 service frequency requirement; (2 stopping plan adjustment; and (3 priority of train types. Train timetabling problem based on time-space path aims to minimize the total general time-space path cost of all trains. An improved branch-and-price algorithm is applied to solve the large scale integer programming problem. When dealing with the algorithm, a rapid branching and node selection for branch-and-price tree and a heuristic train time-space path generation for column generation are adopted to speed up the algorithm computation time. The computational results of a set of experiments on China’s high-speed rail system are presented with the discussions about the model validation, the effectiveness of the general time-space path cost, and the improved branch-and-price algorithm.

ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion.

Science.gov (United States)

Chen, Wei-Ta; Ebelt, Nancy D; Stracker, Travis H; Xhemalce, Blerta; Van Den Berg, Carla L; Miller, Kyle M

2015-06-01

Ataxia-telangiectasia mutated (ATM) protein kinase regulates the DNA damage response (DDR) and is associated with cancer suppression. Here we report a cancer-promoting role for ATM. ATM depletion in metastatic cancer cells reduced cell migration and invasion. Transcription analyses identified a gene network, including the chemokine IL-8, regulated by ATM. IL-8 expression required ATM and was regulated by oxidative stress. IL-8 was validated as an ATM target by its ability to rescue cell migration and invasion defects in ATM-depleted cells. Finally, ATM-depletion in human breast cancer cells reduced lung tumors in a mouse xenograft model and clinical data validated IL-8 in lung metastasis. These findings provide insights into how ATM activation by oxidative stress regulates IL-8 to sustain cell migration and invasion in cancer cells to promote metastatic potential. Thus, in addition to well-established roles in tumor suppression, these findings identify a role for ATM in tumor progression.

Molecular Imaging of the ATM Kinase Activity

Energy Technology Data Exchange (ETDEWEB)

Williams, Terence M. [Department of Radiation Oncology, Ohio State University, Columbus, Ohio (United States); Nyati, Shyam [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Ross, Brian D. [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Rehemtulla, Alnawaz, E-mail: alnawaz@umich.edu [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States)

2013-08-01

Purpose: Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase critical to the cellular DNA-damage response, including from DNA double-strand breaks. ATM activation results in the initiation of a complex cascade of events including DNA damage repair, cell cycle checkpoint control, and survival. We sought to create a bioluminescent reporter that dynamically and noninvasively measures ATM kinase activity in living cells and subjects. Methods and Materials: Using the split luciferase technology, we constructed a hybrid cDNA, ATM-reporter (ATMR), coding for a protein that quantitatively reports on changes in ATM kinase activity through changes in bioluminescence. Results: Treatment of ATMR-expressing cells with ATM inhibitors resulted in a dose-dependent increase in bioluminescence activity. In contrast, induction of ATM kinase activity upon irradiation resulted in a decrease in reporter activity that correlated with ATM and Chk2 activation by immunoblotting in a time-dependent fashion. Nuclear targeting improved ATMR sensitivity to both ATM inhibitors and radiation, whereas a mutant ATMR (lacking the target phosphorylation site) displayed a muted response. Treatment with ATM inhibitors and small interfering (si)RNA-targeted knockdown of ATM confirm the specificity of the reporter. Using reporter expressing xenografted tumors demonstrated the ability of ATMR to report in ATM activity in mouse models that correlated in a time-dependent fashion with changes in Chk2 activity. Conclusions: We describe the development and validation of a novel, specific, noninvasive bioluminescent reporter that enables monitoring of ATM activity in real time, in vitro and in vivo. Potential applications of this reporter include the identification and development of novel ATM inhibitors or ATM-interacting partners through high-throughput screens and in vivo pharmacokinetic/pharmacodynamic studies of ATM inhibitors in preclinical models.

Study on the correlation between the hierarchical urban system and high-speed railway network planning in China

Directory of Open Access Journals (Sweden)

Hong Sun

2016-09-01

Full Text Available This study examines the interrelatedness between the hierarchical structure of China׳s urban system and high-speed railway (HSR network planning at the national level. As a multi-layered system, the Chinese HSR can be categorized into three sub-networks, namely, the national HSR trunk network, the national HSR extensional network, and the intercity HSR network. By examining the direct HSR network connection, HSR nodal connection, and HSR operational frequency of 287 prefecture-level cities, this study demonstrates that the hierarchies of China׳s administrative, demographic, and economic urban systems strongly influence HSR network planning. The national HSR trunk network prioritizes the connection of top-level central cities, whereas the extensional network prioritizes cities at the lower level of the urban system. Moreover, the national HSR system forms the backbone of the HSR network structure based on a national scale, whereas the intercity HSR system satisfies the travel needs within urban agglomerations based on the regional level.

The Passive Microwave Neural Network Precipitation Retrieval (PNPR) for AMSU/MHS and ATMS cross-track scanning radiometers

Science.gov (United States)

Sano', Paolo; Casella, Daniele; Panegrossi, Giulia; Cinzia Marra, Anna; Dietrich, Stefano

2016-04-01

Spaceborne microwave cross-track scanning radiometers, originally developed for temperature and humidity sounding, have shown great capabilities to provide a significant contribution in precipitation monitoring both in terms of measurement quality and spatial/temporal coverage. The Passive microwave Neural network Precipitation Retrieval (PNPR) algorithm for cross-track scanning radiometers, originally developed for the Advanced Microwave Sounding Unit/Microwave Humidity Sounder (AMSU-A/MHS) radiometers (on board the European MetOp and U.S. NOAA satellites), was recently newly designed to exploit the Advanced Technology Microwave Sounder (ATMS) on board the Suomi-NPP satellite and the future JPSS satellites. The PNPR algorithm is based on the Artificial Neural Network (ANN) approach. The main PNPR-ATMS algorithm changes with respect to PNPR-AMSU/MHS are the design and implementation of a new ANN able to manage the information derived from the additional ATMS channels (respect to the AMSU-A/MHS radiometer) and a new screening procedure for not-precipitating pixels. In order to achieve maximum consistency of the retrieved surface precipitation, both PNPR algorithms are based on the same physical foundation. The PNPR is optimized for the European and the African area. The neural network was trained using a cloud-radiation database built upon 94 cloud-resolving simulations over Europe and the Mediterranean and over the African area and radiative transfer model simulations of TB vectors consistent with the AMSU-A/MHS and ATMS channel frequencies, viewing angles, and view-angle dependent IFOV sizes along the scan projections. As opposed to other ANN precipitation retrieval algorithms, PNPR uses a unique ANN that retrieves the surface precipitation rate for all types of surface backgrounds represented in the training database, i.e., land (vegetated or arid), ocean, snow/ice or coast. This approach prevents different precipitation estimates from being inconsistent with one

A High-Speed Train Operation Plan Inspection Simulation Model

Directory of Open Access Journals (Sweden)

Yang Rui

2018-01-01

Full Text Available We developed a train operation simulation tool to inspect a train operation plan. In applying an improved Petri Net, the train was regarded as a token, and the line and station were regarded as places, respectively, in accordance with the high-speed train operation characteristics and network function. Location change and running information transfer of the high-speed train were realized by customizing a variety of transitions. The model was built based on the concept of component combination, considering the random disturbance in the process of train running. The simulation framework can be generated quickly and the system operation can be completed according to the different test requirements and the required network data. We tested the simulation tool when used for the real-world Wuhan to Guangzhou high-speed line. The results showed that the proposed model can be developed, the simulation results basically coincide with the objective reality, and it can not only test the feasibility of the high-speed train operation plan, but also be used as a support model to develop the simulation platform with more capabilities.

The high speed interconnect system architecture and operation

Science.gov (United States)

Anderson, Steven C.

The design and operation of a fiber-optic high-speed interconnect system (HSIS) being developed to meet the requirements of future avionics and flight-control hardware with distributed-system architectures are discussed. The HSIS is intended for 100-Mb/s operation of a local-area network with up to 256 stations. It comprises a bus transmission system (passive star couplers and linear media linked by active elements) and network interface units (NIUs). Each NIU is designed to perform the physical, data link, network, and transport functions defined by the ISO OSI Basic Reference Model (1982 and 1983) and incorporates a fiber-optic transceiver, a high-speed protocol based on the SAE AE-9B linear token-passing data bus (1986), and a specialized application interface unit. The operating modes and capabilities of HSIS are described in detail and illustrated with diagrams.

Transport Flow Control and Connection Admission Policies for Reliable Applications

National Research Council Canada - National Science Library

Lamont, Louise

1996-01-01

The purpose of this report was to determine transport protocol performance characteristics over high speed trans-Atlantic ATM connections, using national High Speed Test Networks and Teleglobe's trans...

ATM-dependent pathways of chromatin remodelling and oxidative DNA damage responses.

Science.gov (United States)

Berger, N Daniel; Stanley, Fintan K T; Moore, Shaun; Goodarzi, Aaron A

2017-10-05

Ataxia-telangiectasia mutated (ATM) is a serine/threonine protein kinase with a master regulatory function in the DNA damage response. In this role, ATM commands a complex biochemical network that signals the presence of oxidative DNA damage, including the dangerous DNA double-strand break, and facilitates subsequent repair. Here, we review the current state of knowledge regarding ATM-dependent chromatin remodelling and epigenomic alterations that are required to maintain genomic integrity in the presence of DNA double-strand breaks and/or oxidative stress. We will focus particularly on the roles of ATM in adjusting nucleosome spacing at sites of unresolved DNA double-strand breaks within complex chromatin environments, and the impact of ATM on preserving the health of cells within the mammalian central nervous system.This article is part of the themed issue 'Chromatin modifiers and remodellers in DNA repair and signalling'. © 2017 The Author(s).

Real-Time, Interactive Echocardiography Over High-Speed Networks: Feasibility and Functional Requirements

Science.gov (United States)

Bobinsky, Eric A.

1998-01-01

Real-time, Interactive Echocardiography Over High Speed Networks: Feasibility and Functional Requirements is an experiment in advanced telemedicine being conducted jointly by the NASA Lewis Research Center, the NASA Ames Research Center, and the Cleveland Clinic Foundation. In this project, a patient undergoes an echocardiographic examination in Cleveland while being diagnosed remotely by a cardiologist in California viewing a real-time display of echocardiographic video images transmitted over the broadband NASA Research and Education Network (NREN). The remote cardiologist interactively guides the sonographer administering the procedure through a two-way voice link between the two sites. Echocardiography is a noninvasive medical technique that applies ultrasound imaging to the heart, providing a "motion picture" of the heart in action. Normally, echocardiographic examinations are performed by a sonographer and cardiologist who are located in the same medical facility as the patient. The goal of telemedicine is to allow medical specialists to examine patients located elsewhere, typically in remote or medically underserved geographic areas. For example, a small, rural clinic might have access to an echocardiograph machine but not a cardiologist. By connecting this clinic to a major metropolitan medical facility through a communications network, a minimally trained technician would be able to carry out the procedure under the supervision and guidance of a qualified cardiologist.

ROS-activated ATM-dependent phosphorylation of cytoplasmic substrates identified by large scale phosphoproteomics screen

DEFF Research Database (Denmark)

Kozlov, Sergei V; Waardenberg, Ashley J; Engholm-Keller, Kasper

2016-01-01

ATM (ataxia-telangiectasia, mutated) protein plays a central role in phosphorylating a network of proteins in response to DNA damage. These proteins function in signalling pathways designed to maintain the stability of the genome and minimize the risk of disease by controlling cell cycle checkpoi......ATM (ataxia-telangiectasia, mutated) protein plays a central role in phosphorylating a network of proteins in response to DNA damage. These proteins function in signalling pathways designed to maintain the stability of the genome and minimize the risk of disease by controlling cell cycle...... checkpoints, initiating DNA repair and regulating gene expression. ATM kinase can be activated by a variety of stimuli, including oxidative stress. Here we confirmed activation of cytoplasmic ATM by autophosphorylation at multiple sites. Then we employed a global quantitative phosphoproteomics approach...... to identify cytoplasmic proteins altered in their phosphorylation state in control and A-T (ataxia-telangiectasia) cells in response to oxidative damage. We demonstrated that ATM was activated by oxidative damage in the cytoplasm as well as in the nucleus and identified a total of 9,833 phosphorylation sites...

ATM (ataxia-telangiectasia mutated) abnormality and diseases

International Nuclear Information System (INIS)

Takagi, Masatoshi; Nakata, Shinichiro; Mizutani, Shuki

2007-01-01

Ataxia-Telangiectasia (A-T) is an autosomal recessive inherited disease due to mutation of ATM gene on chromosome 11q22.3, with major symptoms of ataxia, telangiectasia, immunodeficiency and frequent complication of cancer, and the cells have characters of chromosomal break, high sensitivity to radiation and inappropriate continuation of DNA synthesis after radiation. This review describes past and present studies of ATM functions with clinical features in the following order: Clinical symptoms and epidemiology; ATM gene mutation in A-T patients, mainly by frame-shift (80-90%); ATM, whose gene consisted from 66 exons (150 kb), functions in phosphoinositide-3-kinase related kinase family which protecting cells from stress and integrating their system, at response to DNA double strand break, and in the cell cycle checkpoints at G1/S, S and G2/M phases; ATM nonsense/missense mutations in embryonic cells leading to carcinogenesis and role of ATM in the suppression of carcinogenesis in somatic cells; Chromosomal translocation which relating to carcinogenesis, by functional defect of ATM; and Other functions of ATM in neuronal growth, immunodeficiency, carbohydrate and lipid metabolism, early senescence, and virus infection. ATM is thus an essential molecule to maintain growth and homeostasis. (T.I.)

Learning Traffic as Images: A Deep Convolutional Neural Network for Large-Scale Transportation Network Speed Prediction.

Science.gov (United States)

Ma, Xiaolei; Dai, Zhuang; He, Zhengbing; Ma, Jihui; Wang, Yong; Wang, Yunpeng

2017-04-10

This paper proposes a convolutional neural network (CNN)-based method that learns traffic as images and predicts large-scale, network-wide traffic speed with a high accuracy. Spatiotemporal traffic dynamics are converted to images describing the time and space relations of traffic flow via a two-dimensional time-space matrix. A CNN is applied to the image following two consecutive steps: abstract traffic feature extraction and network-wide traffic speed prediction. The effectiveness of the proposed method is evaluated by taking two real-world transportation networks, the second ring road and north-east transportation network in Beijing, as examples, and comparing the method with four prevailing algorithms, namely, ordinary least squares, k-nearest neighbors, artificial neural network, and random forest, and three deep learning architectures, namely, stacked autoencoder, recurrent neural network, and long-short-term memory network. The results show that the proposed method outperforms other algorithms by an average accuracy improvement of 42.91% within an acceptable execution time. The CNN can train the model in a reasonable time and, thus, is suitable for large-scale transportation networks.

Tug of War between Survival and Death: Exploring ATM Function in Cancer

Science.gov (United States)

Stagni, Venturina; Oropallo, Veronica; Fianco, Giulia; Antonelli, Martina; Cinà, Irene; Barilà, Daniela

2014-01-01

Ataxia-telangiectasia mutated (ATM) kinase is a one of the main guardian of genome stability and plays a central role in the DNA damage response (DDR). The deregulation of these pathways is strongly linked to cancer initiation and progression as well as to the development of therapeutic approaches. These observations, along with reports that identify ATM loss of function as an event that may promote tumor initiation and progression, point to ATM as a bona fide tumor suppressor. The identification of ATM as a positive modulator of several signalling networks that sustain tumorigenesis, including oxidative stress, hypoxia, receptor tyrosine kinase and AKT serine-threonine kinase activation, raise the question of whether ATM function in cancer may be more complex. This review aims to give a complete overview on the work of several labs that links ATM to the control of the balance between cell survival, proliferation and death in cancer. PMID:24681585

Tug of War between Survival and Death: Exploring ATM Function in Cancer

Directory of Open Access Journals (Sweden)

Venturina Stagni

2014-03-01

Full Text Available Ataxia-telangiectasia mutated (ATM kinase is a one of the main guardian of genome stability and plays a central role in the DNA damage response (DDR. The deregulation of these pathways is strongly linked to cancer initiation and progression as well as to the development of therapeutic approaches. These observations, along with reports that identify ATM loss of function as an event that may promote tumor initiation and progression, point to ATM as a bona fide tumor suppressor. The identification of ATM as a positive modulator of several signalling networks that sustain tumorigenesis, including oxidative stress, hypoxia, receptor tyrosine kinase and AKT serine-threonine kinase activation, raise the question of whether ATM function in cancer may be more complex. This review aims to give a complete overview on the work of several labs that links ATM to the control of the balance between cell survival, proliferation and death in cancer.

Wave speed in excitable random networks with spatially constrained connections.

Directory of Open Access Journals (Sweden)

Nikita Vladimirov

Full Text Available Very fast oscillations (VFO in neocortex are widely observed before epileptic seizures, and there is growing evidence that they are caused by networks of pyramidal neurons connected by gap junctions between their axons. We are motivated by the spatio-temporal waves of activity recorded using electrocorticography (ECoG, and study the speed of activity propagation through a network of neurons axonally coupled by gap junctions. We simulate wave propagation by excitable cellular automata (CA on random (Erdös-Rényi networks of special type, with spatially constrained connections. From the cellular automaton model, we derive a mean field theory to predict wave propagation. The governing equation resolved by the Fisher-Kolmogorov PDE fails to describe wave speed. A new (hyperbolic PDE is suggested, which provides adequate wave speed v( that saturates with network degree , in agreement with intuitive expectations and CA simulations. We further show that the maximum length of connection is a much better predictor of the wave speed than the mean length. When tested in networks with various degree distributions, wave speeds are found to strongly depend on the ratio of network moments / rather than on mean degree , which is explained by general network theory. The wave speeds are strikingly similar in a diverse set of networks, including regular, Poisson, exponential and power law distributions, supporting our theory for various network topologies. Our results suggest practical predictions for networks of electrically coupled neurons, and our mean field method can be readily applied for a wide class of similar problems, such as spread of epidemics through spatial networks.

Usage of super high speed computer for clarification of complex phenomena

International Nuclear Information System (INIS)

Sekiguchi, Tomotsugu; Sato, Mitsuhisa; Nakata, Hideki; Tatebe, Osami; Takagi, Hiromitsu

1999-01-01

This study aims at construction of an efficient super high speed computer system application environment in response to parallel distributed system with easy transplantation to different computer system and different number by conducting research and development on super high speed computer application technology required for elucidation of complicated phenomenon in elucidation of complicated phenomenon of nuclear power field due to computed scientific method. In order to realize such environment, the Electrotechnical Laboratory has conducted development on Ninf, a network numerical information library. This Ninf system can supply a global network infrastructure for worldwide computing with high performance on further wide range distributed network (G.K.)

High speed data transmission at the Superconducting Super Collider

International Nuclear Information System (INIS)

Leskovar, B.

1990-04-01

High speed data transmission using fiber optics in the data acquisition system of the Superconducting Super Collider has been investigated. Emphasis is placed on the high speed data transmission system overview, the local data network and on subassemblies, such as optical transmitters and receivers. Also, the performance of candidate subassemblies having a low power dissipation for the data acquisition system is discussed. 14 refs., 5 figs

An HTRF® Assay for the Protein Kinase ATM.

Science.gov (United States)

Adams, Phillip; Clark, Jonathan; Hawdon, Simon; Hill, Jennifer; Plater, Andrew

2017-01-01

Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase that plays a key role in the regulation of DNA damage pathways and checkpoint arrest. In recent years, there has been growing interest in ATM as a therapeutic target due to its association with cancer cell survival following genotoxic stress such as radio- and chemotherapy. Large-scale targeted drug screening campaigns have been hampered, however, by technical issues associated with the production of sufficient quantities of purified ATM and the availability of a suitable high-throughput assay. Using a purified, functionally active recombinant ATM and one of its physiological substrates, p53, we have developed an in vitro FRET-based activity assay that is suitable for high-throughput drug screening.

Cell formation effects on the burning speeds and flame front area of synthetic gas at high pressures and temperatures

International Nuclear Information System (INIS)

Askari, Omid; Elia, Mimmo; Ferrari, Matthew; Metghalchi, Hameed

2017-01-01

Highlights: • Effect of cell formation on burning speed and flame surface area is investigated. • A new developed non-dimensional number called cellularity factor is introduced. • Cellular burning speed and mass burning rate are calculated using differential based multi-shell model. • Flame instability is studied using thermo-diffusive and hydrodynamics effects. • Power law correlations are developed for cellular burning speeds and mass burning rates. - Abstract: Cellular burning speeds and mass burning rates of premixed syngas/oxidizer/diluent (H_2/CO/O_2/He) have been determined at high pressures and temperatures over a wide range of equivalence ratios which are at engine-relevant conditions. Working on high pressure combustion helps to reduce the pollution and increase the energy efficiency in combustion devices. The experimental facilities consisted of two spherical and cylindrical chambers. The spherical chamber, which can withstand high pressures up to 400 atm, was used to collect pressure rise data due to combustion, to calculate cellular burning speed and mass burning rate. For flame structure and instability analysis the cylindrical chamber was used to take pictures of propagating flame using a high speed CMOS camera and a schlieren photography system. A new differential based multi-shell model based on pressure rise data was used to determine the cellular burning speed and mass burning rate. In this paper, cellular burning speed and mass burning rate of H_2/CO/O_2/He mixture have been measured for a wide range of equivalence ratios from 0.6 to 2, temperatures from 400 to 750 K and pressures from 2 to 50 atm for three hydrogen concentrations of 5, 10 and 25% in the syngas. The power law correlations for cellular burning speed and mass burning rate were developed as a function of equivalence ratio, temperature and pressure. In this study a new developed parameter, called cellularity factor, which indicates the cell formation effect on flame

Kinase-dead ATM protein is highly oncogenic and can be preferentially targeted by Topo-isomerase I inhibitors.

Science.gov (United States)

Yamamoto, Kenta; Wang, Jiguang; Sprinzen, Lisa; Xu, Jun; Haddock, Christopher J; Li, Chen; Lee, Brian J; Loredan, Denis G; Jiang, Wenxia; Vindigni, Alessandro; Wang, Dong; Rabadan, Raul; Zha, Shan

2016-06-15

Missense mutations in ATM kinase, a master regulator of DNA damage responses, are found in many cancers, but their impact on ATM function and implications for cancer therapy are largely unknown. Here we report that 72% of cancer-associated ATM mutations are missense mutations that are enriched around the kinase domain. Expression of kinase-dead ATM (Atm(KD/-)) is more oncogenic than loss of ATM (Atm(-/-)) in mouse models, leading to earlier and more frequent lymphomas with Pten deletions. Kinase-dead ATM protein (Atm-KD), but not loss of ATM (Atm-null), prevents replication-dependent removal of Topo-isomerase I-DNA adducts at the step of strand cleavage, leading to severe genomic instability and hypersensitivity to Topo-isomerase I inhibitors. Correspondingly, Topo-isomerase I inhibitors effectively and preferentially eliminate Atm(KD/-), but not Atm-proficientor Atm(-/-) leukemia in animal models. These findings identify ATM kinase-domain missense mutations as a potent oncogenic event and a biomarker for Topo-isomerase I inhibitor based therapy.

Practical End-to-End Performance Testing Tool for High Speed 3G-Based Networks

Science.gov (United States)

Shinbo, Hiroyuki; Tagami, Atsushi; Ano, Shigehiro; Hasegawa, Toru; Suzuki, Kenji

High speed IP communication is a killer application for 3rd generation (3G) mobile systems. Thus 3G network operators should perform extensive tests to check whether expected end-to-end performances are provided to customers under various environments. An important objective of such tests is to check whether network nodes fulfill requirements to durations of processing packets because a long duration of such processing causes performance degradation. This requires testers (persons who do tests) to precisely know how long a packet is hold by various network nodes. Without any tool's help, this task is time-consuming and error prone. Thus we propose a multi-point packet header analysis tool which extracts and records packet headers with synchronized timestamps at multiple observation points. Such recorded packet headers enable testers to calculate such holding durations. The notable feature of this tool is that it is implemented on off-the shelf hardware platforms, i.e., lap-top personal computers. The key challenges of the implementation are precise clock synchronization without any special hardware and a sophisticated header extraction algorithm without any drop.

The Time Lens Concept Applied to Ultra-High-Speed OTDM Signal Processing

DEFF Research Database (Denmark)

Clausen, Anders; Palushani, Evarist; Mulvad, Hans Christian Hansen

2013-01-01

This survey paper presents some of the applications where the versatile time-lens concept successfully can be applied to ultra-high-speed serial systems by offering expected needed functionalities for future optical communication networks.......This survey paper presents some of the applications where the versatile time-lens concept successfully can be applied to ultra-high-speed serial systems by offering expected needed functionalities for future optical communication networks....

A Method for Upper Bounding on Network Access Speed

DEFF Research Database (Denmark)

Knudsen, Thomas Phillip; Patel, A.; Pedersen, Jens Myrup

2004-01-01

This paper presents a method for calculating an upper bound on network access speed growth and gives guidelines for further research experiments and simulations. The method is aimed at providing a basis for simulation of long term network development and resource management.......This paper presents a method for calculating an upper bound on network access speed growth and gives guidelines for further research experiments and simulations. The method is aimed at providing a basis for simulation of long term network development and resource management....

Recent Greenland Thinning from Operation IceBridge ATM and LVIS Data

Science.gov (United States)

Sutterley, T. C.; Velicogna, I.

2015-12-01

We investigate regional thinning rates in Greenland using two Operation IceBridge lidar instruments, the Airborne Topographic Mapper (ATM) and the Land, Vegetation and Ice Sensor (LVIS). IceBridge and Pre-IceBridge ATM data are available from 1993 to present and IceBridge and Pre-Icebridge LVIS data are available from 2007 to present. We compare different techniques for combining the two datasets: overlapping footprints, triangulated irregular network meshing and radial basis functions. We validate the combination for periods with near term overlap of the two instruments. By combining the two lidar datasets, we are able to investigate intra-annual, annual, interannual surface elevation change. We investigate both the high melt season of 2012 and the low melt season of 2013. In addition, the major 2015 IceBridge Arctic campaign provides new crucial data for determining seasonal ice sheet thinning rates. We compare our LVIS/ATM results with surface mass balance outputs from two regional climate models: the Regional Atmospheric Climate Model (RACMO) and the Modèle Atmosphérique Régional (MAR). We also investigate the thinning rates of major outlet glaciers.

High LET Radiation Can Enhance TGF(Beta) Induced EMT and Cross-Talk with ATM Pathways

Science.gov (United States)

Wang, Minli; Hada, Megumi; Huff, Janice; Pluth, Janice M.; Anderson, Janniffer; ONeill, Peter; Cucinotta, Francis A.

2010-01-01

The TGF(Beta) pathway has been shown to regulate or directly interact with the ATM pathway in the response to radiation in mammary epithelial cells. We investigated possible interactions between the TGF(Beta) and ATM pathways following simulated space radiation using hTERT immortalized human esophageal epithelial cells (EPC-hTERT), mink lung epithelial cells (Mv1lu), and several human fibroblast cell lines. TGF(Beta) is a key modulator of the Epithelial-Mesenchymal Transition (EMT), important in cancer progression and metastasis. The implication of EMT by radiation also has several lines of developing evidence, however is poorly understood. The identification of TGF(Beta) induced EMT can be shown in changes to morphology, related gene over expression or down regulation, which can be detected by RT-PCR, and immunostaining and western blotting. In this study, we have observed morphologic and molecular alternations consistent with EMT after Mv1lu cells were treated with TGF(Beta) High LET radiation enhanced TGF(Beta) mediated EMT with a dose as low as 0.1Gy. In order to consider the TGF(Beta) interaction with ATM we used a potent ATM inhibitor Ku55933 and investigated gene expression changes and Smad signaling kinetics. Ku559933 was observed to reverse TGF(Beta) induced EMT, while this was not observed in dual treated cells (radiation+TGF(Beta)). In EPC-hTERT cells, TGF(Beta) alone was not able to induce EMT after 3 days of application. A combined treatment with high LET, however, significantly caused the alteration of EMT markers. To study the function of p53 in the process of EMT, we knocked down P53 through RNA interference. Morphology changes associated with EMT were observed in epithelial cells with silenced p53. Our study indicates: high LET radiation can enhance TGF(Beta) induced EMT; while ATM is triggering the process of TGF(Beta)-induced EMT, p53 might be an essential repressor for EMT phenotypes.

Development of a queue warning system utilizing ATM infrastructure system development and field-testing : final report.

Science.gov (United States)

2017-06-13

MnDOT has already deployed an extensive infrastructure for Active Traffic Management (ATM) on I-35W and I-94 with plans to expand on other segments of the Twin Cities freeway network. The ATM system includes intelligent lane control signals (ILCS) sp...

A Methodology to Integrate Security and Cost-effectiveness in ATM

Directory of Open Access Journals (Sweden)

Francesca Matarese

2014-01-01

prioritizing the threats and proposing cost-effective countermeasures for the weaknesses found. ATM security is concerned with securing ATM assets in order to prevent threats and limit their effects on the overall aviation network. This effect limitation can be achieved by removing the vulnerability from the system and/or increasing the tolerance in case of component failures due to attacks. The security risk assessment methodology proposed is based on what is currently being done by the industry (the International Civil Aviation Organization (ICAO and the International Standard Organization (ISO, etc..

Planning Optimization of the Distributed Antenna System in High-Speed Railway Communication Network Based on Improved Cuckoo Search

Directory of Open Access Journals (Sweden)

Zhaoyu Chen

2018-01-01

Full Text Available The network planning is a key factor that directly affects the performance of the wireless networks. Distributed antenna system (DAS is an effective strategy for the network planning. This paper investigates the antenna deployment in a DAS for the high-speed railway communication networks and formulates an optimization problem which is NP-hard for achieving the optimal deployment of the antennas in the DAS. To solve this problem, a scheme based on an improved cuckoo search based on dimension cells (ICSDC algorithm is proposed. ICSDC introduces the dimension cell mechanism to avoid the internal dimension interferences in order to improve the performance of the algorithm. Simulation results show that the proposed ICSDC-based scheme obtains a lower network cost compared with the uniform network planning method. Moreover, ICSDC algorithm has better performance in terms of the convergence rate and accuracy compared with the conventional cuckoo search algorithm, the particle swarm optimization, and the firefly algorithm.

Study on high-speed cutting parameters optimization of AlMn1Cu based on neural network and genetic algorithm

Directory of Open Access Journals (Sweden)

Zhenhua Wang

2016-04-01

Full Text Available In this article, the cutting parameters optimization method for aluminum alloy AlMn1Cu in high-speed milling was studied in order to properly select the high-speed cutting parameters. First, a back propagation neural network model for predicting surface roughness of AlMn1Cu was proposed. The prediction model can improve the prediction accuracy and well work out the higher-order nonlinear relationship between surface roughness and cutting parameters. Second, considering the constraints of technical requirements on surface roughness, a mathematical model for optimizing cutting parameters based on the Bayesian neural network prediction model of surface roughness was established so as to obtain the maximum machining efficiency. The genetic algorithm adopting the homogeneous design to initialize population as well as steady-state reproduction without duplicates was also presented. The application indicates that the algorithm can effectively avoid precocity, strengthen global optimization, and increase the calculation efficiency. Finally, a case was presented on the application of the proposed cutting parameters optimization algorithm to optimize the cutting parameters.

Effect and Analysis of Sustainable Cell Rate using MPEG video Traffic in ATM Networks

Directory of Open Access Journals (Sweden)

Sakshi Kaushal

2006-04-01

Full Text Available The broadband networks inhibit the capability to carry multiple types of traffic – voice, video and data, but these services need to be controlled according to the traffic contract negotiated at the time of the connection to maintain desired Quality of service. Such control techniques use traffic descriptors to evaluate its performance and effectiveness. In case of Variable Bit Rate (VBR services, Peak Cell Rate (PCR and its Cell Delay Variation Tolerance (CDVTPCR are mandatory descriptors. In addition to these, ATM Forum proposed Sustainable Cell Rate (SCR and its Cell delay variation tolerance (CDVTSCR. In this paper, we evaluated the impact of specific SCR and CDVTSCR values on the Usage Parameter Control (UPC performance in case of measured MPEG traffic for improving the efficiency

CaC in ATM – the Diffuse Method

OpenAIRE

I. Baroňák; M. Vozňák

2006-01-01

Connection Admission Control is an element in the of preclusive mechanisms of ATM management. Its main task is to prevent overloading of the network and to ensure the required quality of service. This means that it has to predict the service of the network and according to its state it can manage both existing and new connections. This paper deals with the diffuse method, a CAC method that enables us to obtain the required results. 

Convergence speed of consensus problems over undirected scale-free networks

International Nuclear Information System (INIS)

Sun Wei; Dou Li-Hua

2010-01-01

Scale-free networks and consensus behaviour among multiple agents have both attracted much attention. To investigate the consensus speed over scale-free networks is the major topic of the present work. A novel method is developed to construct scale-free networks due to their remarkable power-law degree distributions, while preserving the diversity of network topologies. The time cost or iterations for networks to reach a certain level of consensus is discussed, considering the influence from power-law parameters. They are both demonstrated to be reversed power-law functions of the algebraic connectivity, which is viewed as a measurement on convergence speed of the consensus behaviour. The attempts of tuning power-law parameters may speed up the consensus procedure, but it could also make the network less robust over time delay at the same time. Large scale of simulations are supportive to the conclusions. (general)

Comparison of four Adaboost algorithm based artificial neural networks in wind speed predictions

International Nuclear Information System (INIS)

Liu, Hui; Tian, Hong-qi; Li, Yan-fei; Zhang, Lei

2015-01-01

Highlights: • Four hybrid algorithms are proposed for the wind speed decomposition. • Adaboost algorithm is adopted to provide a hybrid training framework. • MLP neural networks are built to do the forecasting computation. • Four important network training algorithms are included in the MLP networks. • All the proposed hybrid algorithms are suitable for the wind speed predictions. - Abstract: The technology of wind speed prediction is important to guarantee the safety of wind power utilization. In this paper, four different hybrid methods are proposed for the high-precision multi-step wind speed predictions based on the Adaboost (Adaptive Boosting) algorithm and the MLP (Multilayer Perceptron) neural networks. In the hybrid Adaboost–MLP forecasting architecture, four important algorithms are adopted for the training and modeling of the MLP neural networks, including GD-ALR-BP algorithm, GDM-ALR-BP algorithm, CG-BP-FR algorithm and BFGS algorithm. The aim of the study is to investigate the promoted forecasting percentages of the MLP neural networks by the Adaboost algorithm’ optimization under various training algorithms. The hybrid models in the performance comparison include Adaboost–GD-ALR-BP–MLP, Adaboost–GDM-ALR-BP–MLP, Adaboost–CG-BP-FR–MLP, Adaboost–BFGS–MLP, GD-ALR-BP–MLP, GDM-ALR-BP–MLP, CG-BP-FR–MLP and BFGS–MLP. Two experimental results show that: (1) the proposed hybrid Adaboost–MLP forecasting architecture is effective for the wind speed predictions; (2) the Adaboost algorithm has promoted the forecasting performance of the MLP neural networks considerably; (3) among the proposed Adaboost–MLP forecasting models, the Adaboost–CG-BP-FR–MLP model has the best performance; and (4) the improved percentages of the MLP neural networks by the Adaboost algorithm decrease step by step with the following sequence of training algorithms as: GD-ALR-BP, GDM-ALR-BP, CG-BP-FR and BFGS

Turbulent Flame Speeds and NOx Kinetics of HHC Fuels with Contaminants and High Dilution Levels

Energy Technology Data Exchange (ETDEWEB)

Petersen, Eric; Krejci, Michael; Mathieu, Olivier; Vissotski, Andrew; Ravi, Sankar; Sikes, Travis; Levacque, Anthony; Aul, Christopher; Peterson, Eric

2011-09-30

This progress report documents the first year of the project, from October 1, 2010 through September 30, 2011. Laminar flame speeds and ignition delay times have been measured for hydrogen and various compositions of H₂/CO (syngas) at elevated pressures and elevated temperatures. Two constant-volume cylindrical vessels were used to visualize the spherical growth of the flame through the use of a schlieren optical setup to measure the laminar flame speed of the mixture. Hydrogen experiments were performed at initial pressures up to 10 atm and initial temperatures up to 443 K. A syngas composition of 50/50 was chosen to demonstrate the effect of carbon monoxide on H₂-O₂ chemical kinetics at standard temperature and pressures up to 10 atm. All atmospheric mixtures were diluted with standard air, while all elevated-pressure experiments were diluted with a He:O₂ of 7:1 to minimize hydrodynamic instabilities. The laminar flame speed measurements of hydrogen and syngas are compared to available literature data over a wide range of equivalence ratios where good agreement can be seen with several data sets. Additionally, an improved chemical kinetics model is shown for all conditions within the current study. The model and the data presented herein agree well, which demonstrates the continual, improved accuracy of the chemical kinetics model. A high-pressure shock tube was used to measure ignition delay times for several baseline compositions of syngas at three pressures across a wide range of temperatures. The compositions of syngas (H₂/CO) presented in this study include 80/20, 50/50, 40/60, 20/80, and 10/90, all of which are compared to previously published ignition delay times from a hydrogen-oxygen mixture to demonstrate the effect of carbon monoxide addition. Generally, an increase in carbon monoxide increases the ignition delay time, but there does seem to be a pressure dependency. At low temperatures and

Aurora-B Mediated ATM Serine 1403 Phosphorylation Is Required For Mitotic ATM Activation and the Spindle Checkpoint

OpenAIRE

Yang, Chunying; Tang, Xi; Guo, Xiaojing; Niikura, Yohei; Kitagawa, Katsumi; Cui, Kemi; Wong, Stephen T.C.; Fu, Li; Xu, Bo

2011-01-01

The ATM kinase plays a critical role in the maintenance of genetic stability. ATM is activated in response to DNA damage and is essential for cell cycle checkpoints. Here, we report that ATM is activated in mitosis in the absence of DNA damage. We demonstrate that mitotic ATM activation is dependent on the Aurora-B kinase and that Aurora-B phosphorylates ATM on serine 1403. This phosphorylation event is required for mitotic ATM activation. Further, we show that loss of ATM function results in...

Dancing on damaged chromatin. Functions of ATM and the RAD50/MRE11/NBS1 complex in cellular responses to DNA damage

International Nuclear Information System (INIS)

Iijima, Kenta; Ohara, Maki; Seki, Ryota; Tauchi, Hiroshi

2008-01-01

In order to preserve and protect genetic information, eukaryotic cells have developed a signaling or communications network to help the cell respond to DNA damage, and ATM and NBS1 are key players in this network. ATM is a protein kinase which is activated immediately after a DNA double strand break (DSB) is formed, and the resulting signal cascade generated in response to cellular DSBs is regulated by post-translational protein modifications such as phosphorylation and acetylation. In addition, to ensure the efficient functioning of DNA repair and cell cycle checkpoints, the highly ordered structure of eukaryotic chromatin must be appropriately altered to permit access of repair-related factors to DNA. These alterations are termed chromatin remodeling, and are executed by a specific remodeling complex in conjunction with histone modifications. Current advances in the molecular analysis of DNA damage responses have shown that the auto-phosphorylation of ATM and the interaction between ATM and NBS1 are key steps for ATM activation, and that the association of ATM and NBS1 is involved in chromatin remodeling. Identification of novel factors which function in ubiquitination (RNF8, Ubc13, Rap80, etc.) has also enabled us to understand more details of the early stages in DNA repair pathways which respond to DSBs. In this review, the focus is on the role of ATM and the RAD50/MRE11/NBS1 complex in DSB response pathways, and their role in DSB repair and in the regulation of chromatin remodeling. (author)

The development and work procedures to super speed network for the growing demands for the network

International Nuclear Information System (INIS)

Nosaki, Nobuhisa; Aoki, Kazuhisa

2001-01-01

Following the recently growing demands for the network, the modification of Local Area Network (LAN) and Wide Area Network (WAN) to super speed network have been reviewed in the Japan Nuclear Cycle Development Institute (JNC). Though the modification of the LAN to super speed network is being developed this year in Heisei 13 fiscal year, it of the WAN was developed in Heisei 12 fiscal year. In spite of various issues, technical reviews and etc., there are a lot of benefits and merit for the super speed network of the WAN and though the modification of it and reduction of the running cost are contradictory to each other, both of those have been achieved in the JNC. This paper introduces the development and work procedures for the WAN. (author)

Comparison of performance between TCP/IP over ATM e ATM nativo

OpenAIRE

Marcelo Silva Freitas

2001-01-01

Com o recente desenvolvimento de tecnologias de redes de altas taxas de transmissão, tais como Asynchronous Transfer Mode (ATM), o problema da carência por largura de banda foi solucionado. A questão atual é a implementação de sistemas que suportem os protocolos ATM de forma nativa e integral. Atualmente tem-se utilizado aplicativos tradicionais baseados nos protocolos TCP(UDP)/IP no topo da pilha de protocolos ATM. Tal modelo traz redundâncias que implicam diretamente em aumento de overhead ...

FY1995 next generation highly parallel database / dataminig server using 100 PC's and ATM switch; 1995 nendo tasudai no pasokon wo ATM ketsugoshita jisedai choheiretsu database mining server no kaihatsu

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-03-01

The objective of the research is first to build a highly parallel processing system using 100 personal computers and an ATM switch. The former is a commodity for computer, while the latter can be regarded as a commodity for future communication systems. Second is to implement parallel relational database management system and parallel data mining system over the 100-PC cluster system. Third is to run decision-support queries typicalto data warehouses, to run association rule mining, and to prove the effectiveness of the proposed architecture as a next generation parallel database/datamining server. Performance/cost ratio of PC is significantly improved compared with workstations and proprietry systems due to its mass production. The cost of ATM switch is also considerably decreasing since ATM is being widely accepted as a communication-on infrastructure. By combining 100 PCs as computing commodities and ATM switch as a communication commodity, we built large sca-le parallel processing system inexpensively. Each mode employs the Pentium Pro CPU and the communication badwidth between PC's is more than 120Mbits/sec. A new parallel relational DBMS is design-ed and implemented. TPC-D, which is a standard benchmark for decision support applicants (100GBytes) is executed. Our system attained much higher performance than current commercial systems which are also much more expensive than ours. In addition, we developed a novel parallel data mining algorithm to extract associate rules. We implemented it in our system and succeeded toattain high performance. Thus it is verified that ATM connected PC cluster is very promising as a next generation platform for large scale database/dataminig server. (NEDO)

Reactive Oxygen Species (ROS)-Activated ATM-Dependent Phosphorylation of Cytoplasmic Substrates Identified by Large-Scale Phosphoproteomics Screen*

Science.gov (United States)

Kozlov, Sergei V.; Waardenberg, Ashley J.; Engholm-Keller, Kasper; Arthur, Jonathan W.; Graham, Mark E.; Lavin, Martin

2016-01-01

Ataxia-telangiectasia, mutated (ATM) protein plays a central role in phosphorylating a network of proteins in response to DNA damage. These proteins function in signaling pathways designed to maintain the stability of the genome and minimize the risk of disease by controlling cell cycle checkpoints, initiating DNA repair, and regulating gene expression. ATM kinase can be activated by a variety of stimuli, including oxidative stress. Here, we confirmed activation of cytoplasmic ATM by autophosphorylation at multiple sites. Then we employed a global quantitative phosphoproteomics approach to identify cytoplasmic proteins altered in their phosphorylation state in control and ataxia-telangiectasia (A-T) cells in response to oxidative damage. We demonstrated that ATM was activated by oxidative damage in the cytoplasm as well as in the nucleus and identified a total of 9,833 phosphorylation sites, including 6,686 high-confidence sites mapping to 2,536 unique proteins. A total of 62 differentially phosphorylated peptides were identified; of these, 43 were phosphorylated in control but not in A-T cells, and 19 varied in their level of phosphorylation. Motif enrichment analysis of phosphopeptides revealed that consensus ATM serine glutamine sites were overrepresented. When considering phosphorylation events, only observed in control cells (not observed in A-T cells), with predicted ATM sites phosphoSerine/phosphoThreonine glutamine, we narrowed this list to 11 candidate ATM-dependent cytoplasmic proteins. Two of these 11 were previously described as ATM substrates (HMGA1 and UIMCI/RAP80), another five were identified in a whole cell extract phosphoproteomic screens, and the remaining four proteins had not been identified previously in DNA damage response screens. We validated the phosphorylation of three of these proteins (oxidative stress responsive 1 (OSR1), HDGF, and ccdc82) as ATM dependent after H2O2 exposure, and another protein (S100A11) demonstrated ATM

Low Ki67/high ATM protein expression in malignant tumors predicts favorable prognosis in a retrospective study of early stage hormone receptor positive breast cancer.

Science.gov (United States)

Feng, Xiaolan; Li, Haocheng; Kornaga, Elizabeth N; Dean, Michelle; Lees-Miller, Susan P; Riabowol, Karl; Magliocco, Anthony M; Morris, Don; Watson, Peter H; Enwere, Emeka K; Bebb, Gwyn; Paterson, Alexander

2016-12-27

This study was designed to investigate the combined influence of ATM and Ki67 on clinical outcome in early stage hormone receptor positive breast cancer (ES-HPBC), particularly in patients with smaller tumors (ATM and Ki67 proteins using fluorescence and brightfield immunohistochemistry respectively, and quantified their expression with digital image analysis. Data on expression levels were subsequently correlated with clinical outcome. Remarkably, ATM expression was useful to stratify the low Ki67 group into subgroups with better or poorer prognosis. Specifically, in the low Ki67 subgroup defined as having smaller tumors and no positive nodes, patients with high ATM expression showed better outcome than those with low ATM, with estimated survival rates of 96% and 89% respectively at 15 years follow up (p = 0.04). Similarly, low-Ki67 patients with smaller tumors, 1-3 positive nodes and high ATM also had significantly better outcomes than their low ATM counterparts, with estimated survival rates of 88% and 46% respectively (p = 0.03) at 15 years follow up. Multivariable analysis indicated that the combination of high ATM and low Ki67 is prognostic of improved survival, independent of tumor size, grade, and lymph node status (p = 0.02). These data suggest that the prognostic value of Ki67 can be improved by analyzing ATM expression in ES-HPBC.

Defining ATM-Independent Functions of the Mre11 Complex with a Novel Mouse Model.

Science.gov (United States)

Balestrini, Alessia; Nicolas, Laura; Yang-Lott, Katherine; Guryanova, Olga A; Levine, Ross L; Bassing, Craig H; Chaudhuri, Jayanta; Petrini, John H J

2016-02-01

The Mre11 complex (Mre11, Rad50, and Nbs1) occupies a central node of the DNA damage response (DDR) network and is required for ATM activation in response to DNA damage. Hypomorphic alleles of MRE11 and NBS1 confer embryonic lethality in ATM-deficient mice, indicating that the complex exerts ATM-independent functions that are essential when ATM is absent. To delineate those functions, a conditional ATM allele (ATM(flox)) was crossed to hypomorphic NBS1 mutants (Nbs1(ΔB/ΔB) mice). Nbs1(ΔB/ΔB) Atm(-/-) hematopoietic cells derived by crossing to vav(cre) were viable in vivo. Nbs1(ΔB/ΔB) Atm(-/-) (VAV) mice exhibited a pronounced defect in double-strand break repair and completely penetrant early onset lymphomagenesis. In addition to repair defects observed, fragile site instability was noted, indicating that the Mre11 complex promotes genome stability upon replication stress in vivo. The data suggest combined influences of the Mre11 complex on DNA repair, as well as the responses to DNA damage and DNA replication stress. A novel mouse model was developed, by combining a vav(cre)-inducible ATM knockout mouse with an NBS1 hypomorphic mutation, to analyze ATM-independent functions of the Mre11 complex in vivo. These data show that the DNA repair, rather than DDR signaling functions of the complex, is acutely required in the context of ATM deficiency to suppress genome instability and lymphomagenesis. ©2015 American Association for Cancer Research.

Different propagation speeds of recalled sequences in plastic spiking neural networks

Science.gov (United States)

Huang, Xuhui; Zheng, Zhigang; Hu, Gang; Wu, Si; Rasch, Malte J.

2015-03-01

Neural networks can generate spatiotemporal patterns of spike activity. Sequential activity learning and retrieval have been observed in many brain areas, and e.g. is crucial for coding of episodic memory in the hippocampus or generating temporal patterns during song production in birds. In a recent study, a sequential activity pattern was directly entrained onto the neural activity of the primary visual cortex (V1) of rats and subsequently successfully recalled by a local and transient trigger. It was observed that the speed of activity propagation in coordinates of the retinotopically organized neural tissue was constant during retrieval regardless how the speed of light stimulation sweeping across the visual field during training was varied. It is well known that spike-timing dependent plasticity (STDP) is a potential mechanism for embedding temporal sequences into neural network activity. How training and retrieval speeds relate to each other and how network and learning parameters influence retrieval speeds, however, is not well described. We here theoretically analyze sequential activity learning and retrieval in a recurrent neural network with realistic synaptic short-term dynamics and STDP. Testing multiple STDP rules, we confirm that sequence learning can be achieved by STDP. However, we found that a multiplicative nearest-neighbor (NN) weight update rule generated weight distributions and recall activities that best matched the experiments in V1. Using network simulations and mean-field analysis, we further investigated the learning mechanisms and the influence of network parameters on recall speeds. Our analysis suggests that a multiplicative STDP rule with dominant NN spike interaction might be implemented in V1 since recall speed was almost constant in an NMDA-dominant regime. Interestingly, in an AMPA-dominant regime, neural circuits might exhibit recall speeds that instead follow the change in stimulus speeds. This prediction could be tested in

Methylation of the ATM promoter in glioma cells alters ionizing radiation sensitivity

International Nuclear Information System (INIS)

Roy, Kanaklata; Wang, Lilin; Makrigiorgos, G. Mike; Price, Brendan D.

2006-01-01

Glioblastomas are among the malignancies most resistant to radiation therapy. In contrast, cells lacking the ATM protein are highly sensitive to ionizing radiation. The relationship between ATM protein expression and radiosensitivity in 3 glioma cell lines was examined. T98G cells exhibited normal levels of ATM protein, whereas U118 and U87 cells had significantly lower levels of ATM and increased (>2-fold) sensitivity to ionizing radiation compared to T98G cells. The ATM promoter was methylated in U87 cells. Demethylation by azacytidine treatment increased ATM protein levels in the U87 cells and decreased their radiosensitivity. In contrast, the ATM promoter in U118 cells was not methylated. Further, expression of exogenous ATM did not significantly alter the radiosensitivity of U118 cells. ATM expression is therefore heterogeneous in the glioma cells examined. In conclusion, methylation of the ATM promoter may account for the variable radiosensitivity and heterogeneous ATM expression in a fraction of glioma cells

NPP ATMS Snowfall Rate Product

Science.gov (United States)

Meng, Huan; Ferraro, Ralph; Kongoli, Cezar; Wang, Nai-Yu; Dong, Jun; Zavodsky, Bradley; Yan, Banghua

2015-01-01

Passive microwave measurements at certain high frequencies are sensitive to the scattering effect of snow particles and can be utilized to retrieve snowfall properties. Some of the microwave sensors with snowfall sensitive channels are Advanced Microwave Sounding Unit (AMSU), Microwave Humidity Sounder (MHS) and Advance Technology Microwave Sounder (ATMS). ATMS is the follow-on sensor to AMSU and MHS. Currently, an AMSU and MHS based land snowfall rate (SFR) product is running operationally at NOAA/NESDIS. Based on the AMSU/MHS SFR, an ATMS SFR algorithm has been developed recently. The algorithm performs retrieval in three steps: snowfall detection, retrieval of cloud properties, and estimation of snow particle terminal velocity and snowfall rate. The snowfall detection component utilizes principal component analysis and a logistic regression model. The model employs a combination of temperature and water vapor sounding channels to detect the scattering signal from falling snow and derive the probability of snowfall (Kongoli et al., 2015). In addition, a set of NWP model based filters is also employed to improve the accuracy of snowfall detection. Cloud properties are retrieved using an inversion method with an iteration algorithm and a two-stream radiative transfer model (Yan et al., 2008). A method developed by Heymsfield and Westbrook (2010) is adopted to calculate snow particle terminal velocity. Finally, snowfall rate is computed by numerically solving a complex integral. NCEP CMORPH analysis has shown that integration of ATMS SFR has improved the performance of CMORPH-Snow. The ATMS SFR product is also being assessed at several NWS Weather Forecast Offices for its usefulness in weather forecast.

Train Stop Scheduling in a High-Speed Rail Network by Utilizing a Two-Stage Approach

Directory of Open Access Journals (Sweden)

Huiling Fu

2012-01-01

Full Text Available Among the most commonly used methods of scheduling train stops are practical experience and various “one-step” optimal models. These methods face problems of direct transferability and computational complexity when considering a large-scale high-speed rail (HSR network such as the one in China. This paper introduces a two-stage approach for train stop scheduling with a goal of efficiently organizing passenger traffic into a rational train stop pattern combination while retaining features of regularity, connectivity, and rapidity (RCR. Based on a three-level station classification definition, a mixed integer programming model and a train operating tactics descriptive model along with the computing algorithm are developed and presented for the two stages. A real-world numerical example is presented using the Chinese HSR network as the setting. The performance of the train stop schedule and the applicability of the proposed approach are evaluated from the perspective of maintaining RCR.

Environmental risks of high-speed railway in China: Public participation, perception and trust

NARCIS (Netherlands)

He, G.; Mol, A.P.J.; Zhang, L.; Lu, Y.

2015-01-01

Two decades ago China entered an era with rapid expansion of transport infrastructure. In an ambitious plan on high-speed railway development, China plans to have the longest high-speed railway network by 2020. Social concerns and anxiety with the adverse environmental and social risks and impacts

A novel application of artificial neural network for wind speed estimation

Science.gov (United States)

Fang, Da; Wang, Jianzhou

2017-05-01

Providing accurate multi-steps wind speed estimation models has increasing significance, because of the important technical and economic impacts of wind speed on power grid security and environment benefits. In this study, the combined strategies for wind speed forecasting are proposed based on an intelligent data processing system using artificial neural network (ANN). Generalized regression neural network and Elman neural network are employed to form two hybrid models. The approach employs one of ANN to model the samples achieving data denoising and assimilation and apply the other to predict wind speed using the pre-processed samples. The proposed method is demonstrated in terms of the predicting improvements of the hybrid models compared with single ANN and the typical forecasting method. To give sufficient cases for the study, four observation sites with monthly average wind speed of four given years in Western China were used to test the models. Multiple evaluation methods demonstrated that the proposed method provides a promising alternative technique in monthly average wind speed estimation.

A transport layer protocol for the future high speed grid computing: SCTP versus fast tcp multihoming

International Nuclear Information System (INIS)

Arshad, M.J.; Mian, M.S.

2010-01-01

TCP (Transmission Control Protocol) is designed for reliable data transfer on the global Internet today. One of its strong points is its use of flow control algorithm that allows TCP to adjust its congestion window if network congestion is occurred. A number of studies and investigations have confirmed that traditional TCP is not suitable for each and every type of application, for example, bulk data transfer over high speed long distance networks. TCP sustained the time of low-capacity and short-delay networks, however, for numerous factors it cannot be capable to efficiently deal with today's growing technologies (such as wide area Grid computing and optical-fiber networks). This research work surveys the congestion control mechanism of transport protocols, and addresses the different issues involved for transferring the huge data over the future high speed Grid computing and optical-fiber networks. This work also presents the simulations to compare the performance of FAST TCP multihoming with SCTP (Stream Control Transmission Protocol) multihoming in high speed networks. These simulation results show that FAST TCP multihoming achieves bandwidth aggregation efficiently and outperforms SCTP multihoming under a similar network conditions. The survey and simulation results presented in this work reveal that multihoming support into FAST TCP does provide a lot of benefits like redundancy, load-sharing and policy-based routing, which largely improves the whole performance of a network and can meet the increasing demand of the future high-speed network infrastructures (such as in Grid computing). (author)

Hyperoxia activates ATM independent from mitochondrial ROS and dysfunction.

Science.gov (United States)

Resseguie, Emily A; Staversky, Rhonda J; Brookes, Paul S; O'Reilly, Michael A

2015-08-01

High levels of oxygen (hyperoxia) are often used to treat individuals with respiratory distress, yet prolonged hyperoxia causes mitochondrial dysfunction and excessive reactive oxygen species (ROS) that can damage molecules such as DNA. Ataxia telangiectasia mutated (ATM) kinase is activated by nuclear DNA double strand breaks and delays hyperoxia-induced cell death through downstream targets p53 and p21. Evidence for its role in regulating mitochondrial function is emerging, yet it has not been determined if mitochondrial dysfunction or ROS activates ATM. Because ATM maintains mitochondrial homeostasis, we hypothesized that hyperoxia induces both mitochondrial dysfunction and ROS that activate ATM. In A549 lung epithelial cells, hyperoxia decreased mitochondrial respiratory reserve capacity at 12h and basal respiration by 48 h. ROS were significantly increased at 24h, yet mitochondrial DNA double strand breaks were not detected. ATM was not required for activating p53 when mitochondrial respiration was inhibited by chronic exposure to antimycin A. Also, ATM was not further activated by mitochondrial ROS, which were enhanced by depleting manganese superoxide dismutase (SOD2). In contrast, ATM dampened the accumulation of mitochondrial ROS during exposure to hyperoxia. Our findings suggest that hyperoxia-induced mitochondrial dysfunction and ROS do not activate ATM. ATM more likely carries out its canonical response to nuclear DNA damage and may function to attenuate mitochondrial ROS that contribute to oxygen toxicity. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

ATM and KAT5 safeguard replicating chromatin against formaldehyde damage

Science.gov (United States)

Ortega-Atienza, Sara; Wong, Victor C.; DeLoughery, Zachary; Luczak, Michal W.; Zhitkovich, Anatoly

2016-01-01

Many carcinogens damage both DNA and protein constituents of chromatin, and it is unclear how cells respond to this compound injury. We examined activation of the main DNA damage-responsive kinase ATM and formation of DNA double-strand breaks (DSB) by formaldehyde (FA) that forms histone adducts and replication-blocking DNA-protein crosslinks (DPC). We found that low FA doses caused a strong and rapid activation of ATM signaling in human cells, which was ATR-independent and restricted to S-phase. High FA doses inactivated ATM via its covalent dimerization and formation of larger crosslinks. FA-induced ATM signaling showed higher CHK2 phosphorylation but much lower phospho-KAP1 relative to DSB inducers. Replication blockage by DPC did not produce damaged forks or detectable amounts of DSB during the main wave of ATM activation, which did not require MRE11. Chromatin-monitoring KAT5 (Tip60) acetyltransferase was responsible for acetylation and activation of ATM by FA. KAT5 and ATM were equally important for triggering of intra-S-phase checkpoint and ATM signaling promoted recovery of normal human cells after low-dose FA. Our results revealed a major role of the KAT5-ATM axis in protection of replicating chromatin against damage by the endogenous carcinogen FA. PMID:26420831

Analysis of Chromosomal Aberrations after Low and High Dose Rate Gamma Irradiation in ATM or NBS Suppressed Human Fibroblast Cells

Science.gov (United States)

Hada, M.; Huff, J. L.; Patel, Z.; Pluth, J. M.; George, K. A.; Cucinotta, F. A.

2009-01-01

A detailed understanding of the biological effects of heavy nuclei is needed for space radiation protection and for cancer therapy. High-LET radiation produces more complex DNA lesions that may be non-repairable or that may require additional processing steps compared to endogenous DSBs, increasing the possibility of misrepair. Interplay between radiation sensitivity, dose, and radiation quality has not been studied extensively. Previously we studied chromosome aberrations induced by low- and high- LET radiation in several cell lines deficient in ATM (ataxia telangactasia mutated; product of the gene that is mutated in ataxia telangiectasia patients) or NBS (nibrin; product of the gene mutated in the Nijmegen breakage syndrome), and gliomablastoma cells that are proficient or lacking in DNA-dependent protein kinase (DNA-PK) activity. We found that the yields of both simple and complex chromosomal aberrations were significantly increased in the DSB repair defective cells compared to normal cells. The increased aberrations observed for the ATM and NBS defective lines was due to a significantly larger quadratic dose-response term compared to normal fibroblasts for both simple and complex aberrations, while the linear dose-response term was significantly higher in NBS cells only for simple exchanges. These results point to the importance of the functions of ATM and NBS in chromatin modifications that function to facilitate correct DSB repair and minimize aberration formation. To further understand the sensitivity differences that were observed in ATM and NBS deficient cells, in this study, chromosomal aberration analysis was performed in normal lung fibroblast cells treated with KU-55933, a specific ATM kinase inhibitor, or Mirin, an MRN complex inhibitor involved in activation of ATM. We are also testing siRNA knockdown of these proteins. Normal and ATM or NBS suppressed cells were irradiated with gamma-rays and chromosomes were collected with a premature chromosome

High speed atom source

International Nuclear Information System (INIS)

Hoshino, Hitoshi.

1990-01-01

In a high speed atom source, since the speed is not identical between ions and electrons, no sufficient neutralizing effect for ionic rays due to the mixing of the ionic rays and the electron rays can be obtained failing to obtain high speed atomic rays at high density. In view of the above, a speed control means is disposed for equalizing the speed of ions forming ionic rays and the speed of electrons forming electron rays. Further, incident angle of the electron rays and/or ionic rays to a magnet or an electrode is made variable. As a result, the relative speed between the ions and the electrons to the processing direction is reduced to zero, in which the probability of association between the ions and the electrons due to the coulomb force is increased to improve the neutralizing efficiency to easily obtain fine and high density high speed electron rays. Further, by varying the incident angle, a track capable of obtaining an ideal mixing depending on the energy of the neutralized ionic rays is formed. Since the high speed electron rays has such high density, they can be irradiated easily to the minute region of the specimen. (N.H.)

High-speed imaging of explosive eruptions: applications and perspectives

Science.gov (United States)

Taddeucci, Jacopo; Scarlato, Piergiorgio; Gaudin, Damien; Capponi, Antonio; Alatorre-Ibarguengoitia, Miguel-Angel; Moroni, Monica

2013-04-01

Explosive eruptions, being by definition highly dynamic over short time scales, necessarily call for observational systems capable of relatively high sampling rates. "Traditional" tools, like as seismic and acoustic networks, have recently been joined by Doppler radar and electric sensors. Recent developments in high-speed camera systems now allow direct visual information of eruptions to be obtained with a spatial and temporal resolution suitable for the analysis of several key eruption processes. Here we summarize the methods employed to gather and process high-speed videos of explosive eruptions, and provide an overview of the several applications of these new type of data in understanding different aspects of explosive volcanism. Our most recent set up for high-speed imaging of explosive eruptions (FAMoUS - FAst, MUltiparametric Set-up,) includes: 1) a monochrome high speed camera, capable of 500 frames per second (fps) at high-definition (1280x1024 pixel) resolution and up to 200000 fps at reduced resolution; 2) a thermal camera capable of 50-200 fps at 480-120x640 pixel resolution; and 3) two acoustic to infrasonic sensors. All instruments are time-synchronized via a data logging system, a hand- or software-operated trigger, and via GPS, allowing signals from other instruments or networks to be directly recorded by the same logging unit or to be readily synchronized for comparison. FAMoUS weights less than 20 kg, easily fits into four, hand-luggage-sized backpacks, and can be deployed in less than 20' (and removed in less than 2', if needed). So far, explosive eruptions have been recorded in high-speed at several active volcanoes, including Fuego and Santiaguito (Guatemala), Stromboli (Italy), Yasur (Vanuatu), and Eyjafiallajokull (Iceland). Image processing and analysis from these eruptions helped illuminate several eruptive processes, including: 1) Pyroclasts ejection. High-speed videos reveal multiple, discrete ejection pulses within a single Strombolian

FY1995 next generation highly parallel database / dataminig server using 100 PC's and ATM switch; 1995 nendo tasudai no pasokon wo ATM ketsugoshita jisedai choheiretsu database mining server no kaihatsu

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-03-01

The objective of the research is first to build a highly parallel processing system using 100 personal computers and an ATM switch. The former is a commodity for computer, while the latter can be regarded as a commodity for future communication systems. Second is to implement parallel relational database management system and parallel data mining system over the 100-PC cluster system. Third is to run decision-support queries typicalto data warehouses, to run association rule mining, and to prove the effectiveness of the proposed architecture as a next generation parallel database/datamining server. Performance/cost ratio of PC is significantly improved compared with workstations and proprietry systems due to its mass production. The cost of ATM switch is also considerably decreasing since ATM is being widely accepted as a communication-on infrastructure. By combining 100 PCs as computing commodities and ATM switch as a communication commodity, we built large sca-le parallel processing system inexpensively. Each mode employs the Pentium Pro CPU and the communication badwidth between PC's is more than 120Mbits/sec. A new parallel relational DBMS is design-ed and implemented. TPC-D, which is a standard benchmark for decision support applicants (100GBytes) is executed. Our system attained much higher performance than current commercial systems which are also much more expensive than ours. In addition, we developed a novel parallel data mining algorithm to extract associate rules. We implemented it in our system and succeeded toattain high performance. Thus it is verified that ATM connected PC cluster is very promising as a next generation platform for large scale database/dataminig server. (NEDO)

AES Cardless Automatic Teller Machine (ATM) Biometric Security System Design Using FPGA Implementation

Science.gov (United States)

Ahmad, Nabihah; Rifen, A. Aminurdin M.; Helmy Abd Wahab, Mohd

2016-11-01

Automated Teller Machine (ATM) is an electronic banking outlet that allows bank customers to complete a banking transactions without the aid of any bank official or teller. Several problems are associated with the use of ATM card such card cloning, card damaging, card expiring, cast skimming, cost of issuance and maintenance and accessing customer account by third parties. The aim of this project is to give a freedom to the user by changing the card to biometric security system to access the bank account using Advanced Encryption Standard (AES) algorithm. The project is implemented using Field Programmable Gate Array (FPGA) DE2-115 board with Cyclone IV device, fingerprint scanner, and Multi-Touch Liquid Crystal Display (LCD) Second Edition (MTL2) using Very High Speed Integrated Circuit Hardware (VHSIC) Description Language (VHDL). This project used 128-bits AES for recommend the device with the throughput around 19.016Gbps and utilized around 520 slices. This design offers a secure banking transaction with a low rea and high performance and very suited for restricted space environments for small amounts of RAM or ROM where either encryption or decryption is performed.

Accuracy Assessment for the Three-Dimensional Coordinates by High-Speed Videogrammetric Measurement

Directory of Open Access Journals (Sweden)

Xianglei Liu

2018-01-01

Full Text Available High-speed CMOS camera is a new kind of transducer to make the videogrammetric measurement for monitoring the displacement of high-speed shaking table structure. The purpose of this paper is to validate the three-dimensional coordinate accuracy of the shaking table structure acquired from the presented high-speed videogrammetric measuring system. In the paper, all of the key intermediate links are discussed, including the high-speed CMOS videogrammetric measurement system, the layout of the control network, the elliptical target detection, and the accuracy validation of final 3D spatial results. Through the accuracy analysis, the submillimeter accuracy can be made for the final the three-dimensional spatial coordinates which certify that the proposed high-speed videogrammetric technique is a better alternative technique which can replace the traditional transducer technique for monitoring the dynamic response for the shaking table structure.

Fabrication and characterization of MCC approved testing material - ATM-1 glass

International Nuclear Information System (INIS)

Wald, J.W.

1985-10-01

The Materials Characterization Center Approved Testing Material ATM-1 is a borosilicate glass that incorporates nonradioactive constituents and uranium to represent high-level waste (HLW) resulting from the reprocessing of commercial nuclear reactor fuel. Its composition is based upon the simulated HLW glass type 76-68 to which depleted uranium has been added as UO 2 . Three separate lots of ATM-1 glass have been fabricated, designated ATM-1a, ATM-1b, and ATM-1c. Limited analyses and microstructural evaluations were conducted on each type. Each lot of ATM-1 glass was produced from a feedstock melted in an air atmosphere at between 1150 to 1200 0 C and cast into stress annealed rectangular bars. Bars of ATM-1a were nominally 1.3 x 1.3 x 7.6 cm (approx.36 g each), bars of ATM-1b were nominally 2 x 2.5 x 17.5 cm (approx.190 g each) and bars of ATM-1c were nominally 1.9 x 1.9 x 15 cm (approx.170 g each). Thirteen bars of ATM-1a, 14 bars of ATM-1b, and 6 bars of ATM-1c were produced. Twelve random samples from each of lots ATM-1a, ATM-1b, and ATM-1c were analyzed. The concentrations (except for U and Cs) were obtained by Inductively-Coupled Argon Plasma Atomic Emission Spectroscopy analysis. Cesium analysis was performed by Atomic Absorption Spectroscopy, while uranium was analyzed by Pulsed Laser Fluorometry. X-ray diffraction analysis of four samples indicated that lot ATM-1a had no detectable crystalline phases (<3 wt %), while ATM-1b and ATM-1c contained approx.3 to 5 wt % iron-chrome spinel crystals. These concentrations of secondary spinel component are not considered uncommon. Scanning electron microscopy examination of fracture surfaces revealed only a random, apparently crystalline, second phase (1-10 μm diam) and a random distribution of small voids or bubbles (approx.1 μm nominal diam)

A High Speed Mobile Communication System implementing Bicasting Architecture on the IP Layer

OpenAIRE

Yamada, Kazuhiro

2012-01-01

Having a broadband connection on high speed rails is something that business travelers want most. Increasing number of passengers is requesting even higher access speeds. We are proposing the Media Convergence System as an ideal communication system for future high speed mobile entities. The Media Convergence System recognizes plural wireless communication media between the ground network and each train, and then traffic is load-balanced over active media which varies according to circumstanc...

Place-making around high-speed railway stations in China

NARCIS (Netherlands)

Dai, G.

2015-01-01

The rapid expansion of the High-Speed Railway (HSR) network in China generates leapfrog urbanization on the urban periphery in the forms of ambitious blueprint plan around the mega hubs. Nevertheless, most of the station areas and spatial extension lack "place quality". The place-making process of

Quantitative and Dynamic Imaging of ATM Kinase Activity.

Science.gov (United States)

Nyati, Shyam; Young, Grant; Ross, Brian Dale; Rehemtulla, Alnawaz

2017-01-01

Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase critical to the cellular DNA-damage response, including DNA double-strand breaks (DSBs). ATM activation results in the initiation of a complex cascade of events facilitating DNA damage repair, cell cycle checkpoint control, and survival. Traditionally, protein kinases have been analyzed in vitro using biochemical methods (kinase assays using purified proteins or immunological assays) requiring a large number of cells and cell lysis. Genetically encoded biosensors based on optical molecular imaging such as fluorescence or bioluminescence have been developed to enable interrogation of kinase activities in live cells with a high signal to background. We have genetically engineered a hybrid protein whose bioluminescent activity is dependent on the ATM-mediated phosphorylation of a substrate. The engineered protein consists of the split luciferase-based protein complementation pair with a CHK2 (a substrate for ATM kinase activity) target sequence and a phospho-serine/threonine-binding domain, FHA2, derived from yeast Rad53. Phosphorylation of the serine residue within the target sequence by ATM would lead to its interaction with the phospho-serine-binding domain, thereby preventing complementation of the split luciferase pair and loss of reporter activity. Bioluminescence imaging of reporter expressing cells in cultured plates or as mouse xenografts provides a quantitative surrogate for ATM kinase activity and therefore the cellular DNA damage response in a noninvasive, dynamic fashion.

ATM Coastal Topography-Mississippi, 2001

Science.gov (United States)

Nayegandhi, Amar; Yates, Xan; Brock, John C.; Sallenger, A.H.; Klipp, Emily S.; Wright, C. Wayne

2009-01-01

These remotely sensed, geographically referenced elevation measurements of lidar-derived first-surface (FS) topography were produced collaboratively by the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL, and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of the Mississippi coastline, from Lakeshore to Petit Bois Island, acquired September 9-10, 2001. The datasets are made available for use as a management tool to research scientists and natural-resource managers. An innovative scanning lidar instrument originally developed by NASA, and known as the Airborne Topographic Mapper (ATM), was used during data acquisition. The ATM system is a scanning lidar system that measures high-resolution topography of the land surface and incorporates a green-wavelength laser operating at pulse rates of 2 to 10 kilohertz. Measurements from the laser-ranging device are coupled with data acquired from inertial navigation system (INS) attitude sensors and differentially corrected global positioning system (GPS) receivers to measure topography of the surface at accuracies of +/-15 centimeters. The nominal ATM platform is a Twin Otter or P-3 Orion aircraft, but the instrument may be deployed on a range of light aircraft. Elevation measurements were collected over the survey area using the ATM system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of lidar data in an interactive or batch mode. Modules for presurvey flight-line definition, flight-path plotting, lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant return within each waveform. ALPS

ATM Coastal Topography-Alabama 2001

Science.gov (United States)

Nayegandhi, Amar; Yates, Xan; Brock, John C.; Sallenger, A.H.; Bonisteel, Jamie M.; Klipp, Emily S.; Wright, C. Wayne

2009-01-01

These remotely sensed, geographically referenced elevation measurements of Lidar-derived first surface (FS) topography were produced collaboratively by the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL, and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of the Alabama coastline, acquired October 3-4, 2001. The datasets are made available for use as a management tool to research scientists and natural resource managers. An innovative scanning Lidar instrument originally developed by NASA, and known as the Airborne Topographic Mapper (ATM), was used during data acquisition. The ATM system is a scanning Lidar system that measures high-resolution topography of the land surface, and incorporates a green-wavelength laser operating at pulse rates of 2 to 10 kilohertz. Measurements from the laser ranging device are coupled with data acquired from inertial navigation system (INS) attitude sensors and differentially corrected global positioning system (GPS) receivers to measure topography of the surface at accuracies of +/-15 centimeters. The nominal ATM platform is a Twin Otter or P-3 Orion aircraft, but the instrument may be deployed on a range of light aircraft. Elevation measurements were collected over the survey area using the ATM system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of Lidar data in an interactive or batch mode. Modules for pre-survey flight line definition, flight path plotting, Lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant return within each waveform. ALPS is routinely used to create maps that

Intel Legend and CERN would build up high speed Internet

CERN Multimedia

2002-01-01

Intel, Legend and China Education and Research Network jointly announced on the 25th of April that they will be cooperating with each other to build up the new generation high speed internet, over the next three years (1/2 page).

Novel targets for ATM-deficient malignancies

Science.gov (United States)

Winkler, Johannes; Hofmann, Kay; Chen, Shuhua

2014-01-01

Conventional chemo- and radiotherapies for the treatment of cancer target rapidly dividing cells in both tumor and non-tumor tissues and can exhibit severe cytotoxicity in normal tissue and impair the patient's immune system. Novel targeted strategies aim for higher efficacy and tumor specificity. The role of ATM protein in the DNA damage response is well known and ATM deficiency frequently plays a role in tumorigenesis and development of malignancy. In addition to contributing to disease development, ATM deficiency also renders malignant cells heavily dependent on other pathways that cooperate with the ATM-mediated DNA damage response to ensure tumor cell survival. Disturbing those cooperative pathways by inhibiting critical protein components allows specific targeting of tumors while sparing healthy cells with normal ATM status. We review druggable candidate targets for the treatment of ATM-deficient malignancies and the mechanisms underlying such targeted therapies. PMID:27308314

OMNET - high speed data communications for PDP-11 computers

International Nuclear Information System (INIS)

Parkman, C.F.; Lee, J.G.

1979-12-01

Omnet is a high speed data communications network designed at CERN for PDP-11 computers. It has grown from a link multiplexor system built for a CII 10070 computer into a full multi-point network, to which some fifty computers are now connected. It provides communications facilities for several large experimental installations as well as many smaller systems and has connections to all parts of the CERN site. The transmission protocol is discussed and brief details are given of the hardware and software used in its implementation. Also described is the gateway interface to the CERN packet switching network, 'Cernet'. (orig.)

Differential Processing of Low and High LET Radiation Induced DNA Damage: Investigation of Switch from ATM to ATR Signaling

Science.gov (United States)

Saha, Janapriya; Wang, Minli; Hada, Megumi; Cucinotta, Francis A.

2011-01-01

The members of the phosphatidylinositol kinase-like kinase family of proteins namely ataxia-telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) are directly responsible for the maintenance of genomic integrity by mounting DDR through signaling and facilitating the recruitment of repair factors at the sites of DNA damage along with coordinating the deployment of cell cycle checkpoints to permit repair by phosphorylating Checkpoint kinase Chk1, Chk2 and p53. High LET radiation from GCR (Galactic Cosmic Rays) consisting mainly of protons and high energy and charged (HZE) particles from SPE (Solar Particle Event) pose a major health risk for astronauts on their space flight missions. The determination of these risks and the design of potential safeguards require sound knowledge of the biological consequences of lesion induction and the capability of the cells to counter them. We here strive to determine the coordination of ATM and ATR kinases at the break sites directly affecting checkpoint signaling and DNA repair and whether differential processing of breaks induced by low and high LET radiation leads to possible augmentation of swap of these damage sensors at the sites of DNA damage. Exposure of cells to IR triggers rapid autophosphorylation of serine-1981 that causes dimer dissociation and initiates monomer formation of ATM. ATM kinase activity depends on the disruption of the dimer, which allows access and phosphorylation of downstream ATM substrates like Chk2. Evidence suggests that ATM is activated by the alterations in higher-order chromatin structure although direct binding of ATM to DSB ends may be a crucial step in its activation. On the other hand, in case of ATR, RPA (replication protein A)-coated ssDNA (single-stranded DNA) generated as a result of stalled DNA replication or during processing of chromosomal lesions is crucial for the localization of ATR to sites of DNA damage in association with ATR-interacting protein (ATRIP). Although the

Design and Construction of a High-speed Network Connecting All the Protein Crystallography Beamlines at the Photon Factory

International Nuclear Information System (INIS)

Matsugaki, Naohiro; Yamada, Yusuke; Igarashi, Noriyuki; Wakatsuki, Soichi

2007-01-01

A private network, physically separated from the facility network, was designed and constructed which covered all the four protein crystallography beamlines at the Photon Factory (PF) and Structural Biology Research Center (SBRC). Connecting all the beamlines in the same network allows for simple authentication and a common working environment for a user who uses multiple beamlines. Giga-bit Ethernet wire-speed was achieved for the communication among the beamlines and SBRC buildings

High-Speed 3D Printing of High-Performance Thermosetting Polymers via Two-Stage Curing.

Science.gov (United States)

Kuang, Xiao; Zhao, Zeang; Chen, Kaijuan; Fang, Daining; Kang, Guozheng; Qi, Hang Jerry

2018-04-01

Design and direct fabrication of high-performance thermosets and composites via 3D printing are highly desirable in engineering applications. Most 3D printed thermosetting polymers to date suffer from poor mechanical properties and low printing speed. Here, a novel ink for high-speed 3D printing of high-performance epoxy thermosets via a two-stage curing approach is presented. The ink containing photocurable resin and thermally curable epoxy resin is used for the digital light processing (DLP) 3D printing. After printing, the part is thermally cured at elevated temperature to yield an interpenetrating polymer network epoxy composite, whose mechanical properties are comparable to engineering epoxy. The printing speed is accelerated by the continuous liquid interface production assisted DLP 3D printing method, achieving a printing speed as high as 216 mm h -1 . It is also demonstrated that 3D printing structural electronics can be achieved by combining the 3D printed epoxy composites with infilled silver ink in the hollow channels. The new 3D printing method via two-stage curing combines the attributes of outstanding printing speed, high resolution, low volume shrinkage, and excellent mechanical properties, and provides a new avenue to fabricate 3D thermosetting composites with excellent mechanical properties and high efficiency toward high-performance and functional applications. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Satellite Communications for ATM

Science.gov (United States)

Shamma, Mohammed A.

2003-01-01

This presentation is an overview on Satellite Communication for the Aeronautical Telecommunication Management (ATM) research. Satellite Communications are being considered by the FAA and NASA as a possible alternative to the present and future ground systems supporting Air Traffic Communications. The international Civil Aviation Organization (ICAO) have in place Standards and Recommended Practices (SARPS) for the Aeronautical Mobile Satellite Services (AMSS) which is mainly derived from the pre-existing Inmarsat service that has been in service since the 1980s. The Working Group A of the Aeronautical Mobile Communication Panel of ICAO has also been investigating SARPS for what is called the Next Generation Satellite Service (NGSS) which conforms less to the Inmarsat based architecture and explores wider options in terms of satellite architectures. Several designs are being proposed by Firms such as Boeing, ESA, NASA that are geared toward full or secondary usage of satellite communications for ATM. Satellite communications for ATM can serve several purposes ranging from primary usage where ground services would play a minimal backup role, to an integrated solution where it will be used to cover services, or areas that are less likely to be supported by the proposed and existing ground infrastructure. Such Integrated roles can include usage of satellite communications for oceanic and remote land areas for example. It also can include relieving the capacity of the ground network by providing broadcast based services of Traffic Information Services messages (TIS-B), or Flight Information Services (FIS-B) which can take a significant portion of the ground system capacity. Additionally, satellite communication can play a backup role to support any needs for ground replacement, or additional needed capacity even after the new digital systems are in place. The additional bandwidth that can be provided via satellite communications can also open the door for many new

Cost-benefit analysis of the ATM automatic deposit service

Directory of Open Access Journals (Sweden)

Ivica Županović

2015-03-01

Full Text Available Bankers and other financial experts have analyzed the value of automated teller machines (ATM in terms of growing consumer demand, rising costs of technology development, decreasing profitability and market share. This paper presents a step-by-step cost-benefit analysis of the ATM automatic deposit service. The first step is to determine user attitudes towards using ATM automatic deposit service by using the Technology Acceptance Model (TAM. The second step is to determine location priorities for ATMs that provide automatic deposit services using the Analytic Hierarchy Process (AHP model. The results of the previous steps enable a highly efficient application of cost-benefit analysis for evaluating costs and benefits of automatic deposit services. To understand fully the proposed procedure outside of theoretical terms, a real-world application of a case study is conducted.

Transition to Asynchronous Transfer Mode (ATM) an Implementation Model for NPS Software Metrics Lab

National Research Council Canada - National Science Library

Carney, Cameron

1999-01-01

With Asynchronous Transfer Mode (ATM), we are experiencing the emergence of a network technology that has the potential of satisfying the requirement for a worldwide standard to allow interoperability of information, regardless...

Identification of p32 as a novel substrate for ATM in heart

International Nuclear Information System (INIS)

Kato, Hisakazu; Takashima, Seiji; Asano, Yoshihiro; Shintani, Yasunori; Yamazaki, Satoru; Seguchi, Osamu; Yamamoto, Hiroyuki; Nakano, Atsushi; Higo, Shuichiro; Ogai, Akiko; Minamino, Tetsuo; Kitakaze, Masafumi; Hori, Masatsugu

2008-01-01

Chemotherapeutic agents to induce DNA damage have been limited to use due to severe side effects of cardiotoxicity. ATM (Ataxia-telangiectasia mutated) is an essential protein kinase in triggering DNA damage responses. However, it is unclear how the ATM-mediated DNA damage responses are involved in the cardiac cell damage. To elucidate these functions in heart, we searched for specific substrates of ATM from mouse heart homogenate. Combining an in vitro phosphorylation following anion-exchange chromatography with purification by reverse-phase high-performance liquid chromatography (HPLC), we successfully identified p32, an ASF/SF2-associated protein, as a novel substrate for ATM. An in vitro kinase assay using recombinant p32 revealed that ATM directly phosphorylated p32. Furthermore, we determined Ser 148 of p32 as an ATM phosphorylation site. Since p32 is known to regulate mRNA splicing and transcription, p32 phosphorylation by ATM might be a new transcriptional regulatory pathway for specific DNA damage responses in heart

DNA damage checkpoint kinase ATM regulates germination and maintains genome stability in seeds.

Science.gov (United States)

Waterworth, Wanda M; Footitt, Steven; Bray, Clifford M; Finch-Savage, William E; West, Christopher E

2016-08-23

Genome integrity is crucial for cellular survival and the faithful transmission of genetic information. The eukaryotic cellular response to DNA damage is orchestrated by the DNA damage checkpoint kinases ATAXIA TELANGIECTASIA MUTATED (ATM) and ATM AND RAD3-RELATED (ATR). Here we identify important physiological roles for these sensor kinases in control of seed germination. We demonstrate that double-strand breaks (DSBs) are rate-limiting for germination. We identify that desiccation tolerant seeds exhibit a striking transcriptional DSB damage response during germination, indicative of high levels of genotoxic stress, which is induced following maturation drying and quiescence. Mutant atr and atm seeds are highly resistant to aging, establishing ATM and ATR as determinants of seed viability. In response to aging, ATM delays germination, whereas atm mutant seeds germinate with extensive chromosomal abnormalities. This identifies ATM as a major factor that controls germination in aged seeds, integrating progression through germination with surveillance of genome integrity. Mechanistically, ATM functions through control of DNA replication in imbibing seeds. ATM signaling is mediated by transcriptional control of the cell cycle inhibitor SIAMESE-RELATED 5, an essential factor required for the aging-induced delay to germination. In the soil seed bank, seeds exhibit increased transcript levels of ATM and ATR, with changes in dormancy and germination potential modulated by environmental signals, including temperature and soil moisture. Collectively, our findings reveal physiological functions for these sensor kinases in linking genome integrity to germination, thereby influencing seed quality, crucial for plant survival in the natural environment and sustainable crop production.

Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies.

Science.gov (United States)

Sun, Yingli; Du, Fengxia

2017-01-01

The activation of ATM is critical in the DNA double strand breaks repair pathway. Acetylation of ATM by Tip60 histone acetyltransferase (HAT) plays a key role in the activation of ATM kinase activity in response to DNA damage. ATM forms a stable complex with Tip60 through the FATC domain of ATM. Tip60 acetylates lysine3016 of ATM, and this acetylation induces the activation of ATM. Several techniques are included in the study of ATM acetylation by Tip60, such as in vitro kinase assay, systematic mutagenesis, western blots. Here, we describe how to study the acetylation of ATM using acetylation-specific antibodies.

ImNet: a fiber optic network with multistar topology for high-speed data transmission

Science.gov (United States)

Vossebuerger, F.; Keizers, Andreas; Soederman, N.; Meyer-Ebrecht, Dietrich

1993-10-01

ImNet is a fiber-optic local area network, which has been developed for high speed image communication in Picture Archiving and Communication Systems (PACS). A comprehensive analysis of image communication requirements in hospitals led to the conclusion that there is a need for networks which are optimized for the transmission of large datafiles. ImNet is optimized for this application in contrast to current-state LANs. ImNet consists of two elements: a link module and a switch module. The point-to-point link module can be up to 4 km by using fiber optic cable. For short distances up to 100 m a cheaper module using shielded twisted pair cable is available. The link module works bi-directionally and handles all protocols up to OSI-Level 3. The data rate per link is up to 140 MBit/s (clock rate 175 MHz). The switch module consists of the control unit and the cross-point-switch array. The array has up to fourteen interfaces for link modules. Up to fourteen data transfers each with a maximal transfer rate of 400 MBit/s can be handled at the same time. Thereby the maximal throughput of a switch module is 5.6 GBit/s. Out of these modules a multi-star network can be built i.e., an arbitrary tree structure of stars. This topology allows multiple transmissions at the same time as long as they do not require identical links. Therefore the overall throughput of ImNet can be a multiple of the datarate per link.

Ataxia-telangiectasia mutated (ATM) silencing promotes neuroblastoma progression through a MYCN independent mechanism

Science.gov (United States)

Mandriota, Stefano J.; Valentijn, Linda J.; Lesne, Laurence; Betts, David R.; Marino, Denis; Boudal-Khoshbeen, Mary; London, Wendy B.; Rougemont, Anne-Laure; Attiyeh, Edward F.; Maris, John M.; Hogarty, Michael D.; Koster, Jan; Molenaar, Jan J.; Versteeg, Rogier

2015-01-01

Neuroblastoma, a childhood cancer with highly heterogeneous biology and clinical behavior, is characterized by genomic aberrations including amplification of MYCN. Hemizygous deletion of chromosome 11q is a well-established, independent marker of poor prognosis. While 11q22-q23 is the most frequently deleted region, the neuroblastoma tumor suppressor in this region remains to be identified. Chromosome bands 11q22-q23 contain ATM, a cell cycle checkpoint kinase and tumor suppressor playing a pivotal role in the DNA damage response. Here, we report that haploinsufficiency of ATM in neuroblastoma correlates with lower ATM expression, event-free survival, and overall survival. ATM loss occurs in high stage neuroblastoma without MYCN amplification. In SK-N-SH, CLB-Ga and GI-ME-N human neuroblastoma cells, stable ATM silencing promotes neuroblastoma progression in soft agar assays, and in subcutaneous xenografts in nude mice. This effect is dependent on the extent of ATM silencing and does not appear to involve MYCN. Our findings identify ATM as a potential haploinsufficient neuroblastoma tumor suppressor, whose inactivation mirrors the increased aggressiveness associated with 11q deletion in neuroblastoma. PMID:26053094

Research of x-ray nondestructive detector for high-speed running conveyor belt with steel wire ropes

Science.gov (United States)

Wang, Junfeng; Miao, Changyun; Wang, Wei; Lu, Xiaocui

2008-03-01

An X-ray nondestructive detector for high-speed running conveyor belt with steel wire ropes is researched in the paper. The principle of X-ray nondestructive testing (NDT) is analyzed, the general scheme of the X-ray nondestructive testing system is proposed, and the nondestructive detector for high-speed running conveyor belt with steel wire ropes is developed. The hardware of system is designed with Xilinx's VIRTEX-4 FPGA that embeds PowerPC and MAC IP core, and its network communication software based on TCP/IP protocol is programmed by loading LwIP to PowerPC. The nondestructive testing of high-speed conveyor belt with steel wire ropes and network transfer function are implemented. It is a strong real-time system with rapid scanning speed, high reliability and remotely nondestructive testing function. The nondestructive detector can be applied to the detection of product line in industry.

Ensemble Nonlinear Autoregressive Exogenous Artificial Neural Networks for Short-Term Wind Speed and Power Forecasting.

Science.gov (United States)

Men, Zhongxian; Yee, Eugene; Lien, Fue-Sang; Yang, Zhiling; Liu, Yongqian

2014-01-01

Short-term wind speed and wind power forecasts (for a 72 h period) are obtained using a nonlinear autoregressive exogenous artificial neural network (ANN) methodology which incorporates either numerical weather prediction or high-resolution computational fluid dynamics wind field information as an exogenous input. An ensemble approach is used to combine the predictions from many candidate ANNs in order to provide improved forecasts for wind speed and power, along with the associated uncertainties in these forecasts. More specifically, the ensemble ANN is used to quantify the uncertainties arising from the network weight initialization and from the unknown structure of the ANN. All members forming the ensemble of neural networks were trained using an efficient particle swarm optimization algorithm. The results of the proposed methodology are validated using wind speed and wind power data obtained from an operational wind farm located in Northern China. The assessment demonstrates that this methodology for wind speed and power forecasting generally provides an improvement in predictive skills when compared to the practice of using an "optimal" weight vector from a single ANN while providing additional information in the form of prediction uncertainty bounds.

ATM-Dependent Hyper-Radiosensitivity in Mammalian Cells Irradiated by Heavy Ions

International Nuclear Information System (INIS)

Xue Lian; Yu Dong; Furusawa, Yoshiya; Cao Jianping; Okayasu, Ryuichi; Fan Saijun

2009-01-01

Purpose: Low-dose hyper-radiosensitivity (HRS) and the later appearing radioresistance (termed induced radioresistance [IRR]) was mainly studied in low linear energy transfer (LET) radiation with survival observation. The aim of this study was to find out whether equivalent hypersensitivity occurred in high LET radiation, and the roles of ataxia telangiectasia mutated (ATM) kinase. Methods and Materials: Survival and mutation were measured by clonogenic assay and HPRT mutation assay. ATM Ser1981 activation was detected by Western blotting and immunofluorescent staining. Pretreatment of specific ATM inhibitor (10 μM KU55933) and activator (20 μg/mL chloroquine) before carbon radiation were adopted to explore the involvement of ATM. The roles of ATM were also investigated in its G2/M checkpoint function with histone H3 phosphorylation analysis and flow cytometric assay, and DNA double strand break (DSB) repair function measured using γ-H2AX foci assay. Results: HRS/IRR was observed with survival and mutation in normal human skin fibroblast cells by carbon ions, while impaired in cells with intrinsic ATM deficiency or normal cells modified with specific ATM activator or inhibitor before irradiation. The dose-response pattern of ATM kinase activation was concordant with the transition from HRS to IRR. The ATM-dependent 'early' G2 checkpoint arrest and DNA DSB repair efficiency could explain the difference between HRS and IRR. Conclusions: These data demonstrate that the HRS/IRR by carbon ion radiation is an ATM-dependent phenomenon in the cellular response to DNA damage.

Study of application technology of ultra-high speed computer to the elucidation of complex phenomena

International Nuclear Information System (INIS)

Sekiguchi, Tomotsugu

1996-01-01

The basic design of numerical information library in the decentralized computer network was explained at the first step of constructing the application technology of ultra-high speed computer to the elucidation of complex phenomena. Establishment of the system makes possible to construct the efficient application environment of ultra-high speed computer system to be scalable with the different computing systems. We named the system Ninf (Network Information Library for High Performance Computing). The summary of application technology of library was described as follows: the application technology of library under the distributed environment, numeric constants, retrieval of value, library of special functions, computing library, Ninf library interface, Ninf remote library and registration. By the system, user is able to use the program concentrating the analyzing technology of numerical value with high precision, reliability and speed. (S.Y.)

High-speed packet filtering utilizing stream processors

Science.gov (United States)

Hummel, Richard J.; Fulp, Errin W.

2009-04-01

Parallel firewalls offer a scalable architecture for the next generation of high-speed networks. While these parallel systems can be implemented using multiple firewalls, the latest generation of stream processors can provide similar benefits with a significantly reduced latency due to locality. This paper describes how the Cell Broadband Engine (CBE), a popular stream processor, can be used as a high-speed packet filter. Results show the CBE can potentially process packets arriving at a rate of 1 Gbps with a latency less than 82 μ-seconds. Performance depends on how well the packet filtering process is translated to the unique stream processor architecture. For example the method used for transmitting data and control messages among the pseudo-independent processor cores has a significant impact on performance. Experimental results will also show the current limitations of a CBE operating system when used to process packets. Possible solutions to these issues will be discussed.

Towards green high capacity optical networks

Science.gov (United States)

Glesk, I.; Mohd Warip, M. N.; Idris, S. K.; Osadola, T. B.; Andonovic, I.

2011-09-01

The demand for fast, secure, energy efficient high capacity networks is growing. It is fuelled by transmission bandwidth needs which will support among other things the rapid penetration of multimedia applications empowering smart consumer electronics and E-businesses. All the above trigger unparallel needs for networking solutions which must offer not only high-speed low-cost "on demand" mobile connectivity but should be ecologically friendly and have low carbon footprint. The first answer to address the bandwidth needs was deployment of fibre optic technologies into transport networks. After this it became quickly obvious that the inferior electronic bandwidth (if compared to optical fiber) will further keep its upper hand on maximum implementable serial data rates. A new solution was found by introducing parallelism into data transport in the form of Wavelength Division Multiplexing (WDM) which has helped dramatically to improve aggregate throughput of optical networks. However with these advancements a new bottleneck has emerged at fibre endpoints where data routers must process the incoming and outgoing traffic. Here, even with the massive and power hungry electronic parallelism routers today (still relying upon bandwidth limiting electronics) do not offer needed processing speeds networks demands. In this paper we will discuss some novel unconventional approaches to address network scalability leading to energy savings via advance optical signal processing. We will also investigate energy savings based on advanced network management through nodes hibernation proposed for Optical IP networks. The hibernation reduces the network overall power consumption by forming virtual network reconfigurations through selective nodes groupings and by links segmentations and partitionings.

NASA's ATM Technology Demonstration-1: Integrated Concept of Arrival Operations

Science.gov (United States)

Baxley, Brian T.; Swenson, Harry N.; Prevot, Thomas; Callantine, Todd J.

2012-01-01

This paper describes operations and procedures envisioned for NASA s Air Traffic Management (ATM) Technology Demonstration #1 (ATD-1). The ATD-1 Concept of Operations (ConOps) demonstration will integrate three NASA technologies to achieve high throughput, fuel-efficient arrival operations into busy terminal airspace. They are Traffic Management Advisor with Terminal Metering (TMA-TM) for precise time-based schedules to the runway and points within the terminal area, Controller-Managed Spacing (CMS) decision support tools for terminal controllers to better manage aircraft delay using speed control, and Flight deck Interval Management (FIM) avionics and flight crew procedures to conduct airborne spacing operations. The ATD-1 concept provides de-conflicted and efficient operations of multiple arrival streams of aircraft, passing through multiple merge points, from top-of-descent (TOD) to touchdown. It also enables aircraft to conduct Optimized Profile Descents (OPDs) from en route altitude to the runway, using primarily speed control to maintain separation and schedule. The ATD-1 project is currently addressing the challenges of integrating the three technologies, and implantation into an operational environment. Goals of the ATD-1 demonstration include increasing the throughput of high-density airports, reducing controller workload, increasing efficiency of arrival operations and the frequency of trajectory-based operations, and promoting aircraft ADS-B equipage.

Secure Communications in High Speed Fiber Optical Networks Using Code Division Multiple Access (CDMA) Transmission

Energy Technology Data Exchange (ETDEWEB)

Han, I; Bond, S; Welty, R; Du, Y; Yoo, S; Reinhardt, C; Behymer, E; Sperry, V; Kobayashi, N

2004-02-12

This project is focused on the development of advanced components and system technologies for secure data transmission on high-speed fiber optic data systems. This work capitalizes on (1) a strong relationship with outstanding faculty at the University of California-Davis who are experts in high speed fiber-optic networks, (2) the realization that code division multiple access (CDMA) is emerging as a bandwidth enhancing technique for fiber optic networks, (3) the realization that CDMA of sufficient complexity forms the basis for almost unbreakable one-time key transmissions, (4) our concepts for superior components for implementing CDMA, (5) our expertise in semiconductor device processing and (6) our Center for Nano and Microtechnology, which is where the majority of the experimental work was done. Here we present a novel device concept, which will push the limits of current technology, and will simultaneously solve system implementation issues by investigating new state-of-the-art fiber technologies. This will enable the development of secure communication systems for the transmission and reception of messages on deployed commercial fiber optic networks, through the CDMA phase encoding of broad bandwidth pulses. CDMA technology has been developed as a multiplexing technology, much like wavelength division multiplexing (WDM) or time division multiplexing (TDM), to increase the potential number of users on a given communication link. A novel application of the techniques created for CDMA is to generate secure communication through physical layer encoding. Physical layer encoding devices are developed which utilize semiconductor waveguides with fast carrier response times to phase encode spectral components of a secure signal. Current commercial technology, most commonly a spatial light modulator, allows phase codes to be changed at rates of only 10's of Hertz ({approx}25ms response). The use of fast (picosecond to nanosecond) carrier dynamics of semiconductors

ATM signaling and 53BP1

International Nuclear Information System (INIS)

Zgheib, Omar; Huyen, Yentram; DiTullio, Richard A.; Snyder, Andrew; Venere, Monica; Stavridi, Elena S.; Halazonetis, Thanos D.

2005-01-01

The ATM (mutated in Ataxia-Telangiectasia) protein kinase is an important player in signaling the presence of DNA double strand breaks (DSBs) in higher eukaryotes. Recent studies suggest that ATM monitors the presence of DNA DSBs indirectly, through DNA DSB-induced changes in chromatin structure. One of the proteins that sense these chromatin structure changes is 53BP1, a DNA damage checkpoint protein conserved in all eukaryotes and the putative ortholog of the S. cerevisiae RAD9 protein. We review here the mechanisms by which ATM is activated in response to DNA DSBs, as well as key ATM substrates that control cell cycle progression, apoptosis and DNA repair

Information processing speed and attention in multiple sclerosis: Reconsidering the Attention Network Test (ANT).

Science.gov (United States)

Roth, Alexandra K; Denney, Douglas R; Lynch, Sharon G

2015-01-01

The Attention Network Test (ANT) assesses attention in terms of discrepancies between response times to items that differ in the burden they place on some facet of attention. However, simple arithmetic difference scores commonly used to capture these discrepancies fail to provide adequate control for information processing speed, leading to distorted findings when patient and control groups differ markedly in the speed with which they process and respond to stimulus information. This study examined attention networks in patients with multiple sclerosis (MS) using simple difference scores, proportional scores, and residualized scores that control for processing speed through statistical regression. Patients with relapsing-remitting (N = 20) or secondary progressive (N = 20) MS and healthy controls (N = 40) of similar age, education, and gender completed the ANT. Substantial differences between patients and controls were found on all measures of processing speed. Patients exhibited difficulties in the executive control network, but only when difference scores were considered. When deficits in information processing speed were adequately controlled using proportional or residualized score, deficits in the alerting network emerged. The effect sizes for these deficits were notably smaller than those for overall information processing speed and were also limited to patients with secondary progressive MS. Deficits in processing speed are more prominent in MS than those involving attention, and when the former are properly accounted for, differences in the latter are confined to the alerting network.

ATM induction insufficiency in a radiosensitive breast-cancer patient

International Nuclear Information System (INIS)

Clarke, R.A.; Fang, Z.H.; Marr, P.J.; Kearsley, J.H.; Papadatos, G.; Lee, C.S.; University of Sydney, Camperdown, NSW

2002-01-01

ATM induction insufficiency in a radiosensitive breast-cancer patient The ataxia telangiectasia (A-T) gene (ATM) is a dominant breast cancer gene with tumour suppressor activity. ATM also regulates cellular sensitivity to ionising radiation (IR) presumably through its role as a facilitator of DNA repair. In normal cells and tissues the ATM protein is rapidly induced by IR to threshold/maximum levels. The kinase function of the ATM protein is also rapidly activated in response to IR. The fact that women carriers of ATM mutations can have an increased risk of developing breast cancer and that many sporadic breast tumours have reduced levels of the ATM protein broadens the scope of ATM's tumour suppressor within the breast. This report describes the downregulation of ATM protein levels in a radiosensitive breast cancer patient. Postinduction ATM levels were up to tenfold lower in the patient's fresh tissues compared to normal controls. These results might indicate a much broader role for ATM anomalies in breast cancer aetiology. Copyright (2002) Blackwell Science Pty Ltd

PID Neural Network Based Speed Control of Asynchronous Motor Using Programmable Logic Controller

Directory of Open Access Journals (Sweden)

MARABA, V. A.

2011-11-01

Full Text Available This paper deals with the structure and characteristics of PID Neural Network controller for single input and single output systems. PID Neural Network is a new kind of controller that includes the advantages of artificial neural networks and classic PID controller. Functioning of this controller is based on the update of controller parameters according to the value extracted from system output pursuant to the rules of back propagation algorithm used in artificial neural networks. Parameters obtained from the application of PID Neural Network training algorithm on the speed model of the asynchronous motor exhibiting second order linear behavior were used in the real time speed control of the motor. Programmable logic controller (PLC was used as real time controller. The real time control results show that reference speed successfully maintained under various load conditions.

Advanced Functionalities for Highly Reliable Optical Networks

DEFF Research Database (Denmark)

An, Yi

This thesis covers two research topics concerning optical solutions for networks e.g. avionic systems. One is to identify the applications for silicon photonic devices for cost-effective solutions in short-range optical networks. The other one is to realise advanced functionalities in order...... to increase the availability of highly reliable optical networks. A cost-effective transmitter based on a directly modulated laser (DML) using a silicon micro-ring resonator (MRR) to enhance its modulation speed is proposed, analysed and experimentally demonstrated. A modulation speed enhancement from 10 Gbit...... interconnects and network-on-chips. A novel concept of all-optical protection switching scheme is proposed, where fault detection and protection trigger are all implemented in the optical domain. This scheme can provide ultra-fast establishment of the protection path resulting in a minimum loss of data...

ATM-deficiency increases genomic instability and metastatic potential in a mouse model of pancreatic cancer.

Science.gov (United States)

Drosos, Yiannis; Escobar, David; Chiang, Ming-Yi; Roys, Kathryn; Valentine, Virginia; Valentine, Marc B; Rehg, Jerold E; Sahai, Vaibhav; Begley, Lesa A; Ye, Jianming; Paul, Leena; McKinnon, Peter J; Sosa-Pineda, Beatriz

2017-09-11

Germline mutations in ATM (encoding the DNA-damage signaling kinase, ataxia-telangiectasia-mutated) increase Familial Pancreatic Cancer (FPC) susceptibility, and ATM somatic mutations have been identified in resected human pancreatic tumors. Here we investigated how Atm contributes to pancreatic cancer by deleting this gene in a murine model of the disease expressing oncogenic Kras (Kras G12D ). We show that partial or total ATM deficiency cooperates with Kras G12D to promote highly metastatic pancreatic cancer. We also reveal that ATM is activated in pancreatic precancerous lesions in the context of DNA damage and cell proliferation, and demonstrate that ATM deficiency leads to persistent DNA damage in both precancerous lesions and primary tumors. Using low passage cultures from primary tumors and liver metastases we show that ATM loss accelerates Kras-induced carcinogenesis without conferring a specific phenotype to pancreatic tumors or changing the status of the tumor suppressors p53, p16 Ink4a and p19 Arf . However, ATM deficiency markedly increases the proportion of chromosomal alterations in pancreatic primary tumors and liver metastases. More importantly, ATM deficiency also renders murine pancreatic tumors highly sensitive to radiation. These and other findings in our study conclusively establish that ATM activity poses a major barrier to oncogenic transformation in the pancreas via maintaining genomic stability.

Robustness of Multiple High Speed TCP CUBIC Connections Under Severe Operating Conditions

DEFF Research Database (Denmark)

Pilimon, Artur; Ruepp, Sarah Renée; Berger, Michael Stübert

2015-01-01

We study the adaptation capabilities and robustness of the high-speed TCP CUBIC algorithm. For this purpose we consider a network environment with variable and high random packet loss and a large Bandwidth-Delay product, shared by multiple heterogeneous TCP connections. The analysis is based on a...

Mode of ATM-dependent suppression of chromosome translocation

Energy Technology Data Exchange (ETDEWEB)

Yamauchi, Motohiro, E-mail: motoyama@nagasaki-u.ac.jp [Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523 (Japan); Suzuki, Keiji; Oka, Yasuyoshi; Suzuki, Masatoshi; Kondo, Hisayoshi; Yamashita, Shunichi [Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523 (Japan)

2011-12-09

Highlights: Black-Right-Pointing-Pointer We addressed how ATM suppresses frequency of chromosome translocation. Black-Right-Pointing-Pointer We found ATM/p53-dependent G1 checkpoint suppresses translocation frequency. Black-Right-Pointing-Pointer We found ATM and DNA-PKcs function in a common pathway to suppress translocation. -- Abstract: It is well documented that deficiency in ataxia telangiectasia mutated (ATM) protein leads to elevated frequency of chromosome translocation, however, it remains poorly understood how ATM suppresses translocation frequency. In the present study, we addressed the mechanism of ATM-dependent suppression of translocation frequency. To know frequency of translocation events in a whole genome at once, we performed centromere/telomere FISH and scored dicentric chromosomes, because dicentric and translocation occur with equal frequency and by identical mechanism. By centromere/telomere FISH analysis, we confirmed that chemical inhibition or RNAi-mediated knockdown of ATM causes 2 to 2.5-fold increase in dicentric frequency at first mitosis after 2 Gy of gamma-irradiation in G0/G1. The FISH analysis revealed that ATM/p53-dependent G1 checkpoint suppresses dicentric frequency, since RNAi-mediated knockdown of p53 elevated dicentric frequency by 1.5-fold. We found ATM also suppresses dicentric occurrence independently of its checkpoint role, as ATM inhibitor showed additional effect on dicentric frequency in the context of p53 depletion and Chk1/2 inactivation. Epistasis analysis using chemical inhibitors revealed that ATM kinase functions in the same pathway that requires kinase activity of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to suppress dicentric frequency. From the results in the present study, we conclude that ATM minimizes translocation frequency through its commitment to G1 checkpoint and DNA double-strand break repair pathway that requires kinase activity of DNA-PKcs.

ATM-induced radiosensitization in vitro and in vivo

International Nuclear Information System (INIS)

Choi, E. K.; Ahn, S. D.; Rhee, Y. H.; Chung, H. S.; Ha, S. W; Song, C. W.; Griffin, R. J.; Park, H. J.

2003-01-01

It has been known that ATM plays a central role in response of cells to ionizing radiation by enhancing DNA repair. We have investigated the feasibility of increasing radiosensitivity of tumor cells with the use of ATM inhibitors such as caffeine, pentoxifylline and wortmannin. Human colorectal cancer RKO.C cells and RKO-ATM cells (RKO cells overexpressing ATM) were used in the present study. The clonogenic cell survival in vitro indicated that RKO-ATM cells were markedly radioresistant than RKO.C cells. Treatment with 3 mM of caffeine significantly increased the radiosensitivity of cells, particulary the RKO-ATM cells, so that the radiosensitivity of RKO.C cells and RKO-ATM cells were almost similar. The radiation induced G2/M arrest in RKO-ATM cells was noticeably longer than that in RKO.C cells and caffeine treatment significantly reduced the length of the radiation induced G2/M arrest in both RKO.C and RKO-ATM cells. Pentoxifylline and wortmannin were also less effective than caffeine to radiosensitize RKO.C or RKO-ATM cells. However, wortmannin was more effective than caffeine against human lung adenocarcinoma A549 cells indicating the efficacy of ATM inhibitor to increase radiosensitivity is cell line dependent. For in vivo study, RKO.C cells were injected s.c. into the hind-leg of BALB/c-nuslc nude mice, and allowed to grow to 130mm3 tumor. The mice were i.p. injected with caffeine solution or saline and the tumors irradiated with 10 Gy of X-rays. The radiation induced growth delay was markedly increased by 1-2 mg/g of caffeine. It was concluded that caffeine increases radiosensitivity of tumor cells by inhibiting ATM kinase function, thereby inhibiting DNA repair, that occurs during the G2/M arrest after radiation

Investigation of the effect of cutting speed on the Surface Roughness parameters in CNC End Milling using Artificial Neural Network

International Nuclear Information System (INIS)

Al Hazza, Muataz H F; Adesta, Erry Y T

2013-01-01

This research presents the effect of high cutting speed on the surface roughness in the end milling process by using the Artificial Neural Network (ANN). An experimental investigation was conducted to measure the surface roughness for end milling. A set of sparse experimental data for finish end milling on AISI H13 at hardness of 48 HRC have been conducted. The artificial neural network (ANN) was applied to simulate and study the effect of high cutting speed on the surface roughness

The Landscape of Somatic Genetic Alterations in Breast Cancers From ATM Germline Mutation Carriers.

Science.gov (United States)

Weigelt, Britta; Bi, Rui; Kumar, Rahul; Blecua, Pedro; Mandelker, Diana L; Geyer, Felipe C; Pareja, Fresia; James, Paul A; Couch, Fergus J; Eccles, Diana M; Blows, Fiona; Pharoah, Paul; Li, Anqi; Selenica, Pier; Lim, Raymond S; Jayakumaran, Gowtham; Waddell, Nic; Shen, Ronglai; Norton, Larry; Wen, Hannah Y; Powell, Simon N; Riaz, Nadeem; Robson, Mark E; Reis-Filho, Jorge S; Chenevix-Trench, Georgia

2018-02-28

Pathogenic germline variants in ataxia-telangiectasia mutated (ATM), a gene that plays a role in DNA damage response and cell cycle checkpoints, confer an increased breast cancer (BC) risk. Here, we investigated the phenotypic characteristics and landscape of somatic genetic alterations in 24 BCs from ATM germline mutation carriers by whole-exome and targeted sequencing. ATM-associated BCs were consistently hormone receptor positive and largely displayed minimal immune infiltrate. Although 79.2% of these tumors exhibited loss of heterozygosity of the ATM wild-type allele, none displayed high activity of mutational signature 3 associated with defective homologous recombination DNA (HRD) repair. No TP53 mutations were found in the ATM-associated BCs. Analysis of an independent data set confirmed that germline ATM variants and TP53 somatic mutations are mutually exclusive. Our findings indicate that ATM-associated BCs often harbor bi-allelic inactivation of ATM, are phenotypically distinct from BRCA1/2-associated BCs, lack HRD-related mutational signatures, and that TP53 and ATM genetic alterations are likely epistatic.

16th Department of Energy Computer Security Group Training Conference: Proceedings

Energy Technology Data Exchange (ETDEWEB)

1994-04-01

Various topic on computer security are presented. Integrity standards, smartcard systems, network firewalls, encryption systems, cryptography, computer security programs, multilevel security guards, electronic mail privacy, the central intelligence agency, internet security, and high-speed ATM networking are typical examples of discussed topics. Individual papers are indexed separately.

High-Speed Optical Wide-Area Data-Communication Network

Science.gov (United States)

Monacos, Steve P.

1994-01-01

Proposed fiber-optic wide-area network (WAN) for digital communication balances input and output flows of data with its internal capacity by routing traffic via dynamically interconnected routing planes. Data transmitted optically through network by wavelength-division multiplexing in synchronous or asynchronous packets. WAN implemented with currently available technology. Network is multiple-ring cyclic shuffle exchange network ensuring traffic reaches its destination with minimum number of hops.

DNA-PKcs, ATM, and ATR Interplay Maintains Genome Integrity during Neurogenesis.

Science.gov (United States)

Enriquez-Rios, Vanessa; Dumitrache, Lavinia C; Downing, Susanna M; Li, Yang; Brown, Eric J; Russell, Helen R; McKinnon, Peter J

2017-01-25

The DNA damage response (DDR) orchestrates a network of cellular processes that integrates cell-cycle control and DNA repair or apoptosis, which serves to maintain genome stability. DNA-PKcs (the catalytic subunit of the DNA-dependent kinase, encoded by PRKDC), ATM (ataxia telangiectasia, mutated), and ATR (ATM and Rad3-related) are related PI3K-like protein kinases and central regulators of the DDR. Defects in these kinases have been linked to neurodegenerative or neurodevelopmental syndromes. In all cases, the key neuroprotective function of these kinases is uncertain. It also remains unclear how interactions between the three DNA damage-responsive kinases coordinate genome stability, particularly in a physiological context. Here, we used a genetic approach to identify the neural function of DNA-PKcs and the interplay between ATM and ATR during neurogenesis. We found that DNA-PKcs loss in the mouse sensitized neuronal progenitors to apoptosis after ionizing radiation because of excessive DNA damage. DNA-PKcs was also required to prevent endogenous DNA damage accumulation throughout the adult brain. In contrast, ATR coordinated the DDR during neurogenesis to direct apoptosis in cycling neural progenitors, whereas ATM regulated apoptosis in both proliferative and noncycling cells. We also found that ATR controls a DNA damage-induced G 2 /M checkpoint in cortical progenitors, independent of ATM and DNA-PKcs. These nonoverlapping roles were further confirmed via sustained murine embryonic or cortical development after all three kinases were simultaneously inactivated. Thus, our results illustrate how DNA-PKcs, ATM, and ATR have unique and essential roles during the DDR, collectively ensuring comprehensive genome maintenance in the nervous system. The DNA damage response (DDR) is essential for prevention of a broad spectrum of different human neurologic diseases. However, a detailed understanding of the DDR at a physiological level is lacking. In contrast to many in

The Impact of High-Speed Rail on Residents’ Travel Behavior and Household Mobility: A Case Study of the Beijing-Shanghai Line, China

Directory of Open Access Journals (Sweden)

Hongsheng Chen

2016-11-01

Full Text Available With the improvement of China’s high-speed rail network, there have been many economic and social benefits for local residents. Based on a questionnaire conducted in stations on the Beijing-Shanghai line, and through an analysis of high-speed rail passenger travel behavior and family relocation, we explored the social effects of high-speed rail. The study found that high-speed rail passengers are mainly young, highly educated, and have a middle to high income. However, with the popularization of high-speed rail, such differences in the social stratum of high-speed rail passengers are expected to disappear. Through an analysis of passenger travel status, we found that the areas surrounding high-speed rail stations are very accessible to the main cities, and are well connected by other public transport. With the emergence of the “high mobility era” based on the high-speed rail network, the separation of workplace and residence and the number of “double city” households are increasing, primarily in the Beijing-Tianjin and Shanghai-Nanjing (especially in Suzhou-Kunshan-Shanghai regions. In addition, high-speed rail introduces the possibility of household mobility, with 22.7% of the respondents in this study having relocated since the Beijing-Shanghai line opened. Household mobility is apparent primarily among big cities, with movement toward nearby cities. We also found that occupation, income, residence time, and schooling of children have a significant impact on households. With the improvement of high-speed rail networks, household mobility will become a common phenomenon and research on domestic mobility will continue to increase.

A Mathematical Study of Accessibility and Cohesion Degree in a High-Speed Rail Station Connected to an Urban Bus Transport Network

Directory of Open Access Journals (Sweden)

Mota Carmen

2017-04-01

Full Text Available In the last twenty years, the implementation of High-Speed Rail (HSR has been one of the major strategies for territorial structuring used by various countries. This model has enhanced the development of countries such as France, Spain, Germany and Japan. At present, the United States and China are also starting to implement this model. Nevertheless, the lack of social and economic profitability of several networks is being increasingly analysed. Many networks located in particular regions serve populations that are not large enough to recover the initial investment. For this reason, it is necessary to evaluate the population served by this transport mode, beyond the number of users. In this sense, it is essential to identify the deficiencies and potentials of implementing a network linked to other secondary networks in a specific territory which can compensate for the so-called tunnel effect. This article proposes to apply a mathematical approach based on graph theory to measure the Degree Accessibility Node (DAN in a constrained Geographic Information System (GIS model. Hence, it would be possible to compare regions, especially medium-sized cities, where the implementation of HSR could represent a qualitative leap due to incorporation into large transport networks. The DAN function uses static and dynamic studies to evaluate the level of connection of stations to secondary transport networks—local public transport in this case. Thus, the impact of high-speed trains could be spread to greater territorial and population ranges. Four cases have been studied, two in Germany (one of them, Fulda, is analysed in depth throughout this article and two in Spain. These two countries were selected since HSR was implemented in the same relative period of time, in comparison with other European countries. Results show relevant differences, suggesting a review of inappropriate policies of transport integration in a city that could weaken the expansion of

A Mathematical Study of Accessibility and Cohesion Degree in a High-Speed Rail Station Connected to an Urban Bus Transport Network

Science.gov (United States)

Mota, Carmen; López, Miguel A.; Martínez-Rodrigo, Arturo

2017-04-01

In the last twenty years, the implementation of High-Speed Rail (HSR) has been one of the major strategies for territorial structuring used by various countries. This model has enhanced the development of countries such as France, Spain, Germany and Japan. At present, the United States and China are also starting to implement this model. Nevertheless, the lack of social and economic profitability of several networks is being increasingly analysed. Many networks located in particular regions serve populations that are not large enough to recover the initial investment. For this reason, it is necessary to evaluate the population served by this transport mode, beyond the number of users. In this sense, it is essential to identify the deficiencies and potentials of implementing a network linked to other secondary networks in a specific territory which can compensate for the so-called tunnel effect. This article proposes to apply a mathematical approach based on graph theory to measure the Degree Accessibility Node (DAN) in a constrained Geographic Information System (GIS) model. Hence, it would be possible to compare regions, especially medium-sized cities, where the implementation of HSR could represent a qualitative leap due to incorporation into large transport networks. The DAN function uses static and dynamic studies to evaluate the level of connection of stations to secondary transport networks—local public transport in this case. Thus, the impact of high-speed trains could be spread to greater territorial and population ranges. Four cases have been studied, two in Germany (one of them, Fulda, is analysed in depth throughout this article) and two in Spain. These two countries were selected since HSR was implemented in the same relative period of time, in comparison with other European countries. Results show relevant differences, suggesting a review of inappropriate policies of transport integration in a city that could weaken the expansion of the positive

Structure of the intact ATM/Tel1 kinase

Science.gov (United States)

Wang, Xuejuan; Chu, Huanyu; Lv, Mengjuan; Zhang, Zhihui; Qiu, Shuwan; Liu, Haiyan; Shen, Xuetong; Wang, Weiwu; Cai, Gang

2016-05-01

The ataxia-telangiectasia mutated (ATM) protein is an apical kinase that orchestrates the multifaceted DNA-damage response. Normally, ATM kinase is in an inactive, homodimer form and is transformed into monomers upon activation. Besides a conserved kinase domain at the C terminus, ATM contains three other structural modules, referred to as FAT, FATC and N-terminal helical solenoid. Here we report the first cryo-EM structure of ATM kinase, which is an intact homodimeric ATM/Tel1 from Schizosaccharomyces pombe. We show that two monomers directly contact head-to-head through the FAT and kinase domains. The tandem N-terminal helical solenoid tightly packs against the FAT and kinase domains. The structure suggests that ATM/Tel1 dimer interface and the consecutive HEAT repeats inhibit the binding of kinase substrates and regulators by steric hindrance. Our study provides a structural framework for understanding the mechanisms of ATM/Tel1 regulation as well as the development of new therapeutic agents.

Sensorless Speed Control of Permanent Magnet Synchronous Motors by Neural Network Algorithm

Directory of Open Access Journals (Sweden)

Ming-Shyan Wang

2014-01-01

Full Text Available The sliding mode control has the merits with respect to the variation of the disturbance and robustness. In this paper, the sensorless sliding-mode observer with least mean squared error approach for permanent magnet synchronous motor (PMSM to detect the rotor position by counter electromotive force and then compute motor speed is designed and implemented. In addition, the neural network control is also used to compensate the PI gain tuning to increase the speed accuracy without regarding the errors of the current measurement and motor noise. In this paper, a digital signal processor TMS320F2812 utilizes its high-speed ADC module to get current feedback information and thus to estimate the rotor position and takes advantage of the built-in modules to achieve SVPWM current control so that the senseless speed control will be accomplished. The correctness and effectiveness of the proposed control system will be verified from the experimental results.

Mobile phone signal exposure triggers a hormesis-like effect in Atm+/+ and Atm-/- mouse embryonic fibroblasts.

Science.gov (United States)

Sun, Chuan; Wei, Xiaoxia; Fei, Yue; Su, Liling; Zhao, Xinyuan; Chen, Guangdi; Xu, Zhengping

2016-11-18

Radiofrequency electromagnetic fields (RF-EMFs) have been classified by the International Agency for Research on Cancer as possible carcinogens to humans; however, this conclusion is based on limited epidemiological findings and lacks solid support from experimental studies. In particular, there are no consistent data regarding the genotoxicity of RF-EMFs. Ataxia telangiectasia mutated (ATM) is recognised as a chief guardian of genomic stability. To address the debate on whether RF-EMFs are genotoxic, we compared the effects of 1,800 MHz RF-EMF exposure on genomic DNA in mouse embryonic fibroblasts (MEFs) with proficient (Atm +/+ ) or deficient (Atm -/- ) ATM. In Atm +/+ MEFs, RF-EMF exposure for 1 h at an average special absorption rate of 4.0 W/kg induced significant DNA single-strand breaks (SSBs) and activated the SSB repair mechanism. This effect reduced the DNA damage to less than that of the background level after 36 hours of exposure. In the Atm -/- MEFs, the same RF-EMF exposure for 12 h induced both SSBs and double-strand breaks and activated the two repair processes, which also reduced the DNA damage to less than the control level after prolonged exposure. The observed phenomenon is similar to the hormesis of a toxic substance at a low dose. To the best of our knowledge, this study is the first to report a hormesis-like effect of an RF-EMF.

FORECASTING OF PASSENGER TRAFFIC UPON IMPLEMENTATION OF HIGH-SPEED RUNNING

Directory of Open Access Journals (Sweden)

M. B. Kurhan

2017-02-01

Full Text Available Purpose. Forecasting of passenger traffic flows in the future is an essential and integral part of the complex process of designing of high-speed network (HSN. HSN direction and its parameters are determined by the volume of passenger traffic, the estimated value of which depends on the economic performance of the country, as well as the material status of citizens living in HSN concentration area, transport mobility of population, development of competing modes of transport and so on. The purpose of this work is to analyse the existing methods of passenger traffic forecasting, to evaluate errors of the existing models concerning determination of traffic volumes and to specify the scientific approach to the development of high-speed rail transport in Ukraine. Methodology. The existing forecasting methods are reduced to the following ones: Delphi approach, extrapolation method, factor and correlation analysis, simulation method. The method described in this paper is based on scientific approaches such as analysis – a comprehensive and detailed study of various aspects of the known forecasting methods, comparing of existing methods for establishing differences and similarities, as well as deduction – use of general knowledge to get the new particular one. Thus, the unified indicators determined for the country as a whole, such as gross domestic product, national income, total population and others cannot be used to forecast the traffic flow on specific areas of HSN construction. Therefore, it is necessary to move from the overall forecast to traffic volume forecast on particular direction. Findings. The conclusions are derived from the analysis of different approaches and methods of passenger flow forecasting. It is proposed to create typical techniques of traffic flow forecasting using modern mathematical methods that would allow avoiding unreasonable decisions and shortening project development time. The resulting recommendations will help

Fast collision resolution for real time services in SDMA based wireless ATM networks

DEFF Research Database (Denmark)

Vornefeld, U.; Schieimer, D.; Walke, B.

1999-01-01

protocol, the influence of SDMA on a contention based access protocol is investigated under collision resolution schemes derived from classical splitting algorithms. Although this work is embedded in the framework of wireless ATM and HIPERLAN/2 systems, the ideas are generally applicable....

ATM in Europe: analysis of current status

DEFF Research Database (Denmark)

1999-01-01

This deliverable provides an overview of the current status of the European market for ATM services. The offer of ATM services by principal operators in Belgium, Denmark, Finland, France, Germany, Greece, Italy, Norway, Portugal, Spain, Sweden and United Kingdom is described. In addition, a number...... of international providers of ATM in Europe are presented....

NOTCH1 Inhibits Activation of ATM by Impairing the Formation of an ATM-FOXO3a-KAT5/Tip60 Complex.

Science.gov (United States)

Adamowicz, Marek; Vermezovic, Jelena; d'Adda di Fagagna, Fabrizio

2016-08-23

The DNA damage response (DDR) signal transduction pathway is responsible for sensing DNA damage and further relaying this signal into the cell. ATM is an apical DDR kinase that orchestrates the activation and the recruitment of downstream DDR factors to induce cell-cycle arrest and repair. We have previously shown that NOTCH1 inhibits ATM activation upon DNA damage, but the underlying mechanism remains unclear. Here, we show that NOTCH1 does not impair ATM recruitment to DNA double-strand breaks (DSBs). Rather, NOTCH1 prevents binding of FOXO3a and KAT5/Tip60 to ATM through a mechanism in which NOTCH1 competes with FOXO3a for ATM binding. Lack of FOXO3a binding to ATM leads to the loss of KAT5/Tip60 association with ATM. Moreover, expression of NOTCH1 or depletion of ATM impairs the formation of the FOXO3a-KAT5/Tip60 protein complex. Finally, we show that pharmacological induction of FOXO3a nuclear localization sensitizes NOTCH1-driven cancers to DNA-damage-induced cell death. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Recent studies on the ATM gene

International Nuclear Information System (INIS)

Lavin, M.F.; Khanna, K.K.; Waters, D.

1996-01-01

Full text: Radiosensitivity is a universal characteristic of ataxia-telangiectasia (A-T), observed after exposure of patients and of cells in culture to radiation. This sensitivity is manifested as higher levels of radiation-induced chromosomal aberrations and reduced survival compared to controls. The gene for A-T was mapped to chromosome 11q 22-23 seven years ago and more recently we have been involved in the cloning of a single gene, ATM (ataxia-telangiectasia mutated), mutated in this syndrome. ATM is a large gene, approximately 150 kb in size, composed of 66 exons and codes for a major mRNA of 13 kb with a predicted open reading frame of 9.135 kb. It is not yet known what activity the ATM gene product possesses, but the ralatedness of this gene sequence to the phosphatidylinositol 3-kinase gene family supports a role for ATM in intracellular signalling. Considerable information is already available on defective signalling through the p53 damage-inducible pathway in A-T. This includes failure to arrest at either the G1/S or G2/M checkpoints as well as radioresistant DNA synthesis. A reduced and/or delayed response in the induction of p53 after exposure of A-T cells to ionizing radiation can account for the defective G1/S checkpoint. More recently we have demonstrated that the ATM gene product is involved in the control of multiple cell cycle checkpoints. Antibodies prepared against ATM peptides demonstrate the presence of a protein 350 kDa in size, which is the predicted size for this protein based on open reading frame of 9 kb. This protein is present both in the nucleus and in the cytoplasm where it is present in vesicular structures. As expected from mutation data the ATM protein is absent in cells from some patients with A-T. The cloning of the ATM gene will allow for screening of radiosensitive patients for mutations in this gene and will provide a means of identifying interacting proteins and thus an understanding of how it functions

ATM localization and gene expression in the adult mouse eye.

Science.gov (United States)

Leemput, Julia; Masson, Christel; Bigot, Karine; Errachid, Abdelmounaim; Dansault, Anouk; Provost, Alexandra; Gadin, Stéphanie; Aoufouchi, Said; Menasche, Maurice; Abitbol, Marc

2009-01-01

High levels of metabolism and oxygen consumption in most adult murine ocular compartments, combined with exposure to light and ultraviolet (UV) radiation, are major sources of oxidative stress, causing DNA damage in ocular cells. Of all mammalian body cells, photoreceptor cells consume the largest amount of oxygen and generate the highest levels of oxidative damage. The accumulation of such damage throughout life is a major factor of aging tissues. Several multiprotein complexes have recently been identified as the major sensors and mediators involved in the maintenance of DNA integrity. The activity of these complexes initially seemed to be restricted to dividing cells, given their ultimate role in major cell cycle checkpoints. However, it was later established that they are also active in post-mitotic cells. Recent findings demonstrate that the DNA damage response (DDR) is essential for the development, maintenance, and normal functioning of the adult central nervous system. One major molecular factor in the DDR is the protein, ataxia telangiectasia mutated (ATM). It is required for the rapid induction of cellular responses to DNA double-strand breaks. These cytotoxic DNA lesions may be caused by oxidative damage. To understand how ATM prevents oxidative stress and participates in the maintenance of genomic integrity and cell viability of the adult retina, we determined the ATM expression patterns and studied its localization in the adult mouse eye. Atm gene expression was analyzed by RT-PCR experiments and its localization by in situ hybridization on adult mouse ocular and cerebellar tissue sections. ATM protein expression was determined by western blot analysis of proteins homogenates extracted from several mouse tissues and its localization by immunohistochemistry experiments performed on adult mouse ocular and cerebellar tissue sections. In addition, subcellular localization was realized by confocal microscopy imaging of ocular tissue sections, with a special

Robust/optimal temperature profile control of a high-speed aerospace vehicle using neural networks.

Science.gov (United States)

Yadav, Vivek; Padhi, Radhakant; Balakrishnan, S N

2007-07-01

An approximate dynamic programming (ADP)-based suboptimal neurocontroller to obtain desired temperature for a high-speed aerospace vehicle is synthesized in this paper. A 1-D distributed parameter model of a fin is developed from basic thermal physics principles. "Snapshot" solutions of the dynamics are generated with a simple dynamic inversion-based feedback controller. Empirical basis functions are designed using the "proper orthogonal decomposition" (POD) technique and the snapshot solutions. A low-order nonlinear lumped parameter system to characterize the infinite dimensional system is obtained by carrying out a Galerkin projection. An ADP-based neurocontroller with a dual heuristic programming (DHP) formulation is obtained with a single-network-adaptive-critic (SNAC) controller for this approximate nonlinear model. Actual control in the original domain is calculated with the same POD basis functions through a reverse mapping. Further contribution of this paper includes development of an online robust neurocontroller to account for unmodeled dynamics and parametric uncertainties inherent in such a complex dynamic system. A neural network (NN) weight update rule that guarantees boundedness of the weights and relaxes the need for persistence of excitation (PE) condition is presented. Simulation studies show that in a fairly extensive but compact domain, any desired temperature profile can be achieved starting from any initial temperature profile. Therefore, the ADP and NN-based controllers appear to have the potential to become controller synthesis tools for nonlinear distributed parameter systems.

The telomeric protein TRF2 binds the ATM kinase and can inhibit the ATM-dependent DNA damage response.

Directory of Open Access Journals (Sweden)

Jan Karlseder

2004-08-01

Full Text Available The telomeric protein TRF2 is required to prevent mammalian telomeres from activating DNA damage checkpoints. Here we show that overexpression of TRF2 affects the response of the ATM kinase to DNA damage. Overexpression of TRF2 abrogated the cell cycle arrest after ionizing radiation and diminished several other readouts of the DNA damage response, including phosphorylation of Nbs1, induction of p53, and upregulation of p53 targets. TRF2 inhibited autophosphorylation of ATM on S1981, an early step in the activation of this kinase. A region of ATM containing S1981 was found to directly interact with TRF2 in vitro, and ATM immunoprecipitates contained TRF2. We propose that TRF2 has the ability to inhibit ATM activation at telomeres. Because TRF2 is abundant at chromosome ends but not elsewhere in the nucleus, this mechanism of checkpoint control could specifically block a DNA damage response at telomeres without affecting the surveillance of chromosome internal damage.

Verifying cell loss requirements in high-speed communication networks

Directory of Open Access Journals (Sweden)

Kerry W. Fendick

1998-01-01

Full Text Available In high-speed communication networks it is common to have requirements of very small cell loss probabilities due to buffer overflow. Losses are measured to verify that the cell loss requirements are being met, but it is not clear how to interpret such measurements. We propose methods for determining whether or not cell loss requirements are being met. A key idea is to look at the stream of losses as successive clusters of losses. Often clusters of losses, rather than individual losses, should be regarded as the important “loss events”. Thus we propose modeling the cell loss process by a batch Poisson stochastic process. Successive clusters of losses are assumed to arrive according to a Poisson process. Within each cluster, cell losses do not occur at a single time, but the distance between losses within a cluster should be negligible compared to the distance between clusters. Thus, for the purpose of estimating the cell loss probability, we ignore the spaces between successive cell losses in a cluster of losses. Asymptotic theory suggests that the counting process of losses initiating clusters often should be approximately a Poisson process even though the cell arrival process is not nearly Poisson. The batch Poisson model is relatively easy to test statistically and fit; e.g., the batch-size distribution and the batch arrival rate can readily be estimated from cell loss data. Since batch (cluster sizes may be highly variable, it may be useful to focus on the number of batches instead of the number of cells in a measurement interval. We also propose a method for approximately determining the parameters of a special batch Poisson cell loss with geometric batch-size distribution from a queueing model of the buffer content. For this step, we use a reflected Brownian motion (RBM approximation of a G/D/1/C queueing model. We also use the RBM model to estimate the input burstiness given the cell loss rate. In addition, we use the RBM model to

OPTIMIZATION OF ATM AND BRANCH CASH OPERATIONS USING AN INTEGRATED CASH REQUIREMENT FORECASTING AND CASH OPTIMIZATION MODEL

OpenAIRE

Canser BİLİR

2018-01-01

In this study, an integrated cash requirement forecasting and cash inventory optimization model is implemented in both the branch and automated teller machine (ATM) networks of a mid-sized bank in Turkey to optimize the bank’s cash supply chain. The implemented model’s objective is to minimize the idle cash levels at both branches and ATMs without decreasing the customer service level (CSL) by providing the correct amount of cash at the correct location and time. To the best of our knowledge,...

Energy Efficiency and Capacity Tradeoff in Cloud Radio Access Network of High-Speed Railways

Directory of Open Access Journals (Sweden)

Shichao Li

2017-01-01

Full Text Available To meet the increasing demand of high-data-rate services of high-speed railway (HSR passengers, cloud radio access network (C-RAN is proposed. This paper investigates the tradeoff between energy efficiency (EE performance and capacity in C-RAN of HSR. Considering that the train location can be predicted, we propose a predictable path loss based time domain power allocation method (PPTPA to improve EE performance of HSR communication system. First, we consider that the communication system of HSR only bears the passenger information services (PISs. The energy-efficient power allocation problem with delay constraint is studied. The formulated problem is nonconvex. To deal with it, an equivalent convex problem is reformulated. Based on PPTPA, we propose an iterative algorithm to improve the EE performance. Second, we consider that the PISs and the train control services (TCSs are all bore. A capacity optimization problem with joint EE and services transmission delay constraints is formulated. Based on PPTPA, we propose a hybrid power allocation scheme to improve the capacity of the system. Finally, we analyze the effect of small-scale fading on EE performance. The effectiveness of the proposed power allocation algorithm is validated by HSR channel measurement trace based emulation results and extensive simulation results.

Loss of tumour-specific ATM protein expression is an independent prognostic factor in early resected NSCLC.

Science.gov (United States)

Petersen, Lars F; Klimowicz, Alexander C; Otsuka, Shannon; Elegbede, Anifat A; Petrillo, Stephanie K; Williamson, Tyler; Williamson, Chris T; Konno, Mie; Lees-Miller, Susan P; Hao, Desiree; Morris, Don; Magliocco, Anthony M; Bebb, D Gwyn

2017-06-13

Ataxia-telangiectasia mutated (ATM) is critical in maintaining genomic integrity. In response to DNA double-strand breaks, ATM phosphorylates downstream proteins involved in cell-cycle checkpoint arrest, DNA repair, and apoptosis. Here we investigate the frequency, and influence of ATM deficiency on outcome, in early-resected non-small cell lung cancer (NSCLC). Tissue microarrays, containing 165 formalin-fixed, paraffin-embedded resected NSCLC tumours from patients diagnosed at the Tom Baker Cancer Centre, Calgary, Canada, between 2003 and 2006, were analyzed for ATM expression using quantitative fluorescence immunohistochemistry. Both malignant cell-specific ATM expression and the ratio of ATM expression within malignant tumour cells compared to that in the surrounding tumour stroma, defined as the ATM expression index (ATM-EI), were measured and correlated with clinical outcome. ATM loss was identified in 21.8% of patients, and was unaffected by clinical pathological variables. Patients with low ATM-EI tumours had worse survival outcomes compared to those with high ATM-EI (p ATM-deficient patients may derive greater benefit from guideline-recommended adjuvant chemotherapy following surgical resection. Taken together, these results indicate that ATM loss seems to be an early event in NSCLC carcinogenesis and is an independent prognostic factor associated with worse survival in stage II/III patients.

Twenty years of high-speed lines in Spain; Zwanzig Jahre Hochgeschwindigkeitsstrecken in Spanien

Energy Technology Data Exchange (ETDEWEB)

Vega Vega, Tomas [ADIF, Madrid (Spain); Gil Ortiz, Jose Miguel [Balfour Beatty Rail Iberica, Alcobendas (Spain)

2012-07-15

In April 1992, i.e. 20 years ago, fast train services started in Spain when high-speed trains were put into operation on the almost 500 km long Madrid-Cordoba-Sevilla railway line. Both the 1,435 mm UIC standard gauge and the 1 AC 25 kV 50 Hz overhead line voltage level meant a big step in the development. Today the Spanish high-speed network covers some 2,200 km of railway line, and more lines are planned or already under construction. (orig.)

Probabilistic Safety Analysis of High Speed and Conventional Lines Using Bayesian Networks

Energy Technology Data Exchange (ETDEWEB)

Grande Andrade, Z.; Castillo Ron, E.; O' Connor, A.; Nogal, M.

2016-07-01

A Bayesian network approach is presented for probabilistic safety analysis (PSA) of railway lines. The idea consists of identifying and reproducing all the elements that the train encounters when circulating along a railway line, such as light and speed limit signals, tunnel or viaduct entries or exits, cuttings and embankments, acoustic sounds received in the cabin, curves, switches, etc. In addition, since the human error is very relevant for safety evaluation, the automatic train protection (ATP) systems and the driver behavior and its time evolution are modelled and taken into account to determine the probabilities of human errors. The nodes of the Bayesian network, their links and the associated probability tables are automatically constructed based on the line data that need to be carefully given. The conditional probability tables are reproduced by closed formulas, which facilitate the modelling and the sensitivity analysis. A sorted list of the most dangerous elements in the line is obtained, which permits making decisions about the line safety and programming maintenance operations in order to optimize them and reduce the maintenance costs substantially. The proposed methodology is illustrated by its application to several cases that include real lines such as the Palencia-Santander and the Dublin-Belfast lines. (Author)

ATM Coastal Topography-Florida 2001: Eastern Panhandle

Science.gov (United States)

Yates, Xan; Nayegandhi, Amar; Brock, John C.; Sallenger, A.H.; Bonisteel, Jamie M.; Klipp, Emily S.; Wright, C. Wayne

2009-01-01

These remotely sensed, geographically referenced elevation measurements of Lidar-derived first surface (FS) topography were produced collaboratively by the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL, and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of the eastern Florida panhandle coastline, acquired October 2, 2001. The datasets are made available for use as a management tool to research scientists and natural resource managers. An innovative scanning Lidar instrument originally developed by NASA, and known as the Airborne Topographic Mapper (ATM), was used during data acquisition. The ATM system is a scanning Lidar system that measures high-resolution topography of the land surface and incorporates a green-wavelength laser operating at pulse rates of 2 to 10 kilohertz. Measurements from the laser-ranging device are coupled with data acquired from inertial navigation system (INS) attitude sensors and differentially corrected global positioning system (GPS) receivers to measure topography of the surface at accuracies of +/-15 centimeters. The nominal ATM platform is a Twin Otter or P-3 Orion aircraft, but the instrument may be deployed on a range of light aircraft. Elevation measurements were collected over the survey area using the ATM system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of Lidar data in an interactive or batch mode. Modules for presurvey flight line definition, flight path plotting, Lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant return within each waveform. ALPS is routinely used to create

ATM Coastal Topography-Florida 2001: Western Panhandle

Science.gov (United States)

Yates, Xan; Nayegandhi, Amar; Brock, John C.; Sallenger, A.H.; Bonisteel, Jamie M.; Klipp, Emily S.; Wright, C. Wayne

2009-01-01

These remotely sensed, geographically referenced elevation measurements of Lidar-derived first surface (FS) topography were produced collaboratively by the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL, and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of the western Florida panhandle coastline, acquired October 2-4 and 7-10, 2001. The datasets are made available for use as a management tool to research scientists and natural resource managers. An innovative scanning Lidar instrument originally developed by NASA, and known as the Airborne Topographic Mapper (ATM), was used during data acquisition. The ATM system is a scanning Lidar system that measures high-resolution topography of the land surface and incorporates a green-wavelength laser operating at pulse rates of 2 to 10 kilohertz. Measurements from the laser-ranging device are coupled with data acquired from inertial navigation system (INS) attitude sensors and differentially corrected global positioning system (GPS) receivers to measure topography of the surface at accuracies of +/-15 centimeters. The nominal ATM platform is a Twin Otter or P-3 Orion aircraft, but the instrument may be deployed on a range of light aircraft. Elevation measurements were collected over the survey area using the ATM system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of Lidar data in an interactive or batch mode. Modules for presurvey flight line definition, flight path plotting, Lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant return within each waveform. ALPS is routinely used

Optimization of Trip-end Networks and Ride Price for Express Coach Systems in the High-speed Rail Era

Directory of Open Access Journals (Sweden)

Zhongzhen Yang

2017-12-01

Full Text Available Express coach (EC lost a considerable share of passengers after high-speed rail (HSR was implemented. This paper proposes a door-to-door operation mode for the EC system and builds a model to design an EC trip-end network in the origin city with the aim of maximizing the EC’s daily operating profit. A case study is undertaken, and the results show that the operating profit of the EC system first increases and then decreases with the growth of the trip-end routes. In the HSR era, door-to-door operation can effectively guarantee the market share and operating profits of the EC.

Susceptibility of ATM-deficient pancreatic cancer cells to radiation.

Science.gov (United States)

Ayars, Michael; Eshleman, James; Goggins, Michael

2017-05-19

Ataxia telangiectasia mutated (ATM) is inactivated in a significant minority of pancreatic ductal adenocarcinomas and may be predictor of treatment response. We determined if ATM deficiency renders pancreatic cancer cells more sensitive to fractionated radiation or commonly used chemotherapeutics. ATM expression was knocked down in three pancreatic cancer cell lines using ATM-targeting shRNA. Isogenic cell lines were tested for sensitivity to several chemotherapeutic agents and radiation. DNA repair kinetics were analyzed in irradiated cells using the comet assay. We find that while rendering pancreatic cancer cells ATM-deficient did not significantly change their sensitivity to several chemotherapeutics, it did render them exquisitely sensitized to radiation. Pancreatic cancer ATM status may help predict response to radiotherapy.

Toward Bulk Synchronous Parallel-Based Machine Learning Techniques for Anomaly Detection in High-Speed Big Data Networks

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Kamran Siddique

2017-09-01

Full Text Available Anomaly detection systems, also known as intrusion detection systems (IDSs, continuously monitor network traffic aiming to identify malicious actions. Extensive research has been conducted to build efficient IDSs emphasizing two essential characteristics. The first is concerned with finding optimal feature selection, while another deals with employing robust classification schemes. However, the advent of big data concepts in anomaly detection domain and the appearance of sophisticated network attacks in the modern era require some fundamental methodological revisions to develop IDSs. Therefore, we first identify two more significant characteristics in addition to the ones mentioned above. These refer to the need for employing specialized big data processing frameworks and utilizing appropriate datasets for validating system’s performance, which is largely overlooked in existing studies. Afterwards, we set out to develop an anomaly detection system that comprehensively follows these four identified characteristics, i.e., the proposed system (i performs feature ranking and selection using information gain and automated branch-and-bound algorithms respectively; (ii employs logistic regression and extreme gradient boosting techniques for classification; (iii introduces bulk synchronous parallel processing to cater computational requirements of high-speed big data networks; and; (iv uses the Infromation Security Centre of Excellence, of the University of Brunswick real-time contemporary dataset for performance evaluation. We present experimental results that verify the efficacy of the proposed system.

Synchronous and Asynchronous ATM Multiplexor Properties Comparsion

OpenAIRE

Jan Zabka

2006-01-01

The article is aimed to ATM multiplexor computer model utilisation. Based on simulation runs we try to review aspects of use a synchronous and asynchronous ATM multiplexors. ATM multiplexor is the input queuing model with three inputs. Synchronous multiplexor works without an input priority. Multiplexor inputs are served periodically. Asynchronous multiplexor model supports several queuing and priority mechanisms. CLR and CTD are basic performance parameters. Input cell flows are genera...

ATM-induced radiosensitization in vitro and in vivo

International Nuclear Information System (INIS)

Song, C. W.; Griffin, R. J.; Park, H. J.; Chung, H. S.; Choi, E. K.; Ahn, S. D.; Rhee, Y. H.; Ha, S. W.

2002-01-01

It has been known that ATM plays a central role in response of cells to ionizing radiation by enhancing DNA repair. Based in large part on studies of the homologous proteins in yeast, it is predicted that ATM function as proximal signal transducers in G1, S, and G2 checkpoint pathways. With the exception of p53, the downstream components of these pathways remain largely undefined. We have investigated the feasibility of increasing radiosensitivity of tumor cells with the use of ATM inhibitors such as caffeine, pentoxifylline, and wortmannin. Also in an effort to examine and understand the molecular mechanism by which ATM might exert its cellular effects, we have expressed the full length wild type ATM in RKO cells

OPTIMIZATION OF ATM AND BRANCH CASH OPERATIONS USING AN INTEGRATED CASH REQUIREMENT FORECASTING AND CASH OPTIMIZATION MODEL

Directory of Open Access Journals (Sweden)

Canser BİLİR

2018-04-01

Full Text Available In this study, an integrated cash requirement forecasting and cash inventory optimization model is implemented in both the branch and automated teller machine (ATM networks of a mid-sized bank in Turkey to optimize the bank’s cash supply chain. The implemented model’s objective is to minimize the idle cash levels at both branches and ATMs without decreasing the customer service level (CSL by providing the correct amount of cash at the correct location and time. To the best of our knowledge, the model is the first integrated model in the literature to be applied to both ATMs and branches simultaneously. The results demonstrated that the integrated model dramatically decreased the idle cash levels at both branches and ATMs without degrading the availability of cash and hence customer satisfaction. An in-depth analysis of the results also indicated that the results were more remarkable for branches. The results also demonstrated that the utilization of various seasonal indices plays a very critical role in the forecasting of cash requirements for a bank. Another unique feature of the study is that the model is the first to include the recycling feature of ATMs. The results demonstrated that as a result of the inclusion of the deliberate seasonal indices in the forecasting model, the integrated cash optimization models can be used to estimate the cash requirements of recycling ATMs.

High-speed AC motors

Energy Technology Data Exchange (ETDEWEB)

Jokinen, T.; Arkkio, A. [Helsinki University of Technology Laboratory of Electromechanics, Otaniemi (Finland)

1997-12-31

The paper deals with various types of highspeed electric motors, and their limiting powers. Standard machines with laminated rotors can be utilised if the speed is moderate. The solid rotor construction makes it possible to reach higher power and speed levels than those of laminated rotors. The development work on high-speed motors done at Helsinki University of Technology is presented, too. (orig.) 12 refs.

ATM protein is deficient in over 40% of lung adenocarcinomas.

Science.gov (United States)

Villaruz, Liza C; Jones, Helen; Dacic, Sanja; Abberbock, Shira; Kurland, Brenda F; Stabile, Laura P; Siegfried, Jill M; Conrads, Thomas P; Smith, Neil R; O'Connor, Mark J; Pierce, Andrew J; Bakkenist, Christopher J

2016-09-06

Lung cancer is the leading cause of cancer-related mortality in the USA and worldwide, and of the estimated 1.2 million new cases of lung cancer diagnosed every year, over 30% are lung adenocarcinomas. The backbone of 1st-line systemic therapy in the metastatic setting, in the absence of an actionable oncogenic driver, is platinum-based chemotherapy. ATM and ATR are DNA damage signaling kinases activated at DNA double-strand breaks (DSBs) and stalled and collapsed replication forks, respectively. ATM protein is lost in a number of cancer cell lines and ATR kinase inhibitors synergize with cisplatin to resolve xenograft models of ATM-deficient lung cancer. We therefore sought to determine the frequency of ATM loss in a tissue microarray (TMA) of lung adenocarcinoma. Here we report the validation of a commercial antibody (ab32420) for the identification of ATM by immunohistochemistry and estimate that 61 of 147 (41%, 95% CI 34%-50%) cases of lung adenocarcinoma are negative for ATM protein expression. As a positive control for ATM staining, nuclear ATM protein was identified in stroma and immune infiltrate in all evaluable cases. ATM loss in lung adenocarcinoma was not associated with overall survival. However, our preclinical findings in ATM-deficient cell lines suggest that ATM could be a predictive biomarker for synergy of an ATR kinase inhibitor with standard-of-care cisplatin. This could improve clinical outcome in 100,000's of patients with ATM-deficient lung adenocarcinoma every year.

The new Passive microwave Neural network Precipitation Retrieval (PNPR algorithm for the cross-track scanning ATMS radiometer: description and verification study over Europe and Africa using GPM and TRMM spaceborne radars

Directory of Open Access Journals (Sweden)

P. Sanò

2016-11-01

vegetated land. The results showed a slight tendency to underestimate moderate to high precipitation, mostly over land, and overestimate moderate to light precipitation over ocean. Similar results were obtained for the comparison with GPM-KuPR over the European area (15 months, from March 2014 to May 2015 of coincident overpasses with slightly lower CC (0.59 over vegetated land and 0.57 over ocean and RMSE (0.82 mm h−1 over vegetated land and 0.71 mm h−1 over ocean, confirming a good agreement also between PNPR v2 and GPM-KuPR. The performance of PNPR v2 over the African area was also compared to that of PNPR v1. PNPR v2 has higher R over the different surfaces, with generally better estimation of low precipitation, mostly over ocean, thanks to improvements in the design of the neural network and also to the improved capabilities of ATMS compared to AMSU/MHS. Both versions of PNPR algorithm have shown a general consistency with the TRMM-PR.

ATM Card Cloning and Ethical Considerations.

Science.gov (United States)

Kaur, Paramjit; Krishan, Kewal; Sharma, Suresh K; Kanchan, Tanuj

2018-05-01

With the advent of modern technology, the way society handles and performs monetary transactions has changed tremendously. The world is moving swiftly towards the digital arena. The use of Automated Teller Machine (ATM) cards (credit and debit) has led to a "cash-less society" and has fostered digital payments and purchases. In addition to this, the trust and reliance of the society upon these small pieces of plastic, having numbers engraved upon them, has increased immensely over the last two decades. In the past few years, the number of ATM fraud cases has increased exponentially. With the money of the people shifting towards the digital platform, ATM skimming has become a problem that has eventually led to a global outcry. The present review discusses the serious repercussions of ATM card cloning and the associated privacy, ethical and legal concerns. The preventive measures which need to be taken and adopted by the government authorities to mitigate the problem have also been discussed.

MPLS y ATM como tecnologías backbone para la transferencia de videoconferencia

Directory of Open Access Journals (Sweden)

Danilo López

2012-09-01

Full Text Available This article presents the results obtained by modeling the performance of a core IP/ATM network compared to a CRLDP/IP/MPLS network when carrying delay sensitive traffic (videoconferencing with minimum resource reservation guarantees and optimal interaction between user and applications. In order conduct the experiments, OPNET-modeler simulation software (educational version was used, which encouraged alternative implementation features that help decide which of the two technologies represents a suitable solution for current networking problems, where large volumes of information need to be transmitted and the QoS offered to average users is pretty poor.

Real-time services in IP network architectures

Science.gov (United States)

Gilardi, Antonella

1996-12-01

The worldwide internet system seems to be the success key for the provision of real time multimedia services to both residential and business users and someone says that in such a way broadband networks will have a reason to exist. This new class of applications that use multiple media (voice, video and data) impose constraints to the global network nowadays consisting of subnets with various data links. The attention will be focused on the interconnection of IP non ATM and ATM networks. IETF and ATM forum are currently involved in the developing specifications suited to adapt the connectionless IP protocol to the connection oriented ATM protocol. First of all the link between the ATM and the IP service model has to be set in order to match the QoS and traffic requirements defined in the relative environment. A further significant topic is represented by the mapping of IP resource reservation model onto the ATM signalling and in the end it is necessary to define how the routing works when there are QoS parameters associated. This paper, considering only unicast applications, will examine the above issues taking as a starting point the situation where an host launches as call set up request with the relevant QoS and traffic descriptor and at some point a router at the edge of the ATM network has to decide how forwarding and request in order to establish an end to end link with the right capabilities. The aim is to compare the proposals emerging from different standard bodies to point out convergency or incompatibility.

Study of ATM Phosphorylation by Cdk5 in Neuronal Cells.

Science.gov (United States)

She, Hua; Mao, Zixu

2017-01-01

The phosphatidylinositol-3-kinase-like kinase ATM (ataxia-telangiectasia mutated) plays a central role in coordinating the DNA damage responses including cell cycle checkpoint control, DNA repair, and apoptosis. Mutations of ATM cause a spectrum of defects ranging from neurodegeneration to cancer predisposition. We previously showed that Cdk5 (cyclin-dependent kinase 5) is activated by DNA damage and directly phosphorylates ATM at serine 794 in postmitotic neurons. Phosphorylation at serine 794 precedes and is required for ATM autophosphorylation at serine 1981, and activates ATM kinase activity. Cdk5-ATM pathway plays a crucial role in DNA damage-induced neuronal injury. This chapter describes protocols used in analyzing ATM phosphorylation by Cdk5 in CGNs (cerebellar granule neurons) and its effects on neuronal survival.

A pharmacological screen for compounds that rescue the developmental lethality of a Drosophila ATM mutant.

Science.gov (United States)

Rimkus, Stacey A; Wassarman, David A

2018-01-01

Ataxia-telangiectasia (A-T) is a neurodegenerative disease caused by mutation of the A-T mutated (ATM) gene. ATM encodes a protein kinase that is activated by DNA damage and phosphorylates many proteins, including those involved in DNA repair, cell cycle control, and apoptosis. Characteristic biological and molecular functions of ATM observed in mammals are conserved in Drosophila melanogaster. As an example, conditional loss-of-function ATM alleles in flies cause progressive neurodegeneration through activation of the innate immune response. However, unlike in mammals, null alleles of ATM in flies cause lethality during development. With the goals of understanding biological and molecular roles of ATM in a whole animal and identifying candidate therapeutics for A-T, we performed a screen of 2400 compounds, including FDA-approved drugs, natural products, and bioactive compounds, for modifiers of the developmental lethality caused by a temperature-sensitive ATM allele (ATM8) that has reduced kinase activity at non-permissive temperatures. Ten compounds reproducibly suppressed the developmental lethality of ATM8 flies, including Ronnel, which is an organophosphate. Ronnel and other suppressor compounds are known to cause mitochondrial dysfunction or to inhibit the enzyme acetylcholinesterase, which controls the levels of the neurotransmitter acetylcholine, suggesting that detrimental consequences of reduced ATM kinase activity can be rescued by inhibiting the function of mitochondria or increasing acetylcholine levels. We carried out further studies of Ronnel because, unlike the other compounds that suppressed the developmental lethality of homozygous ATM8 flies, Ronnel was toxic to the development of heterozygous ATM8 flies. Ronnel did not affect the innate immune response of ATM8 flies, and it further increased the already high levels of DNA damage in brains of ATM8 flies, but its effects were not harmful to the lifespan of rescued ATM8 flies. These results provide

A Method for Upper Bounding Long Term Growth of Network Access Speed

Directory of Open Access Journals (Sweden)

Thomas Phillip Knudsen

2006-06-01

Full Text Available The development in home Internet access speed has shown an exponential development with growth rates averaging 25% per year. For resource management in network provisioning it becomes an urgent question how long such growth can continue. This paper presents a method for calculating an upper bound to visual content driven growth, proceeding from datarate requirements for a full virtual environment. Scenarios and approaches for reducing datarate requirements are considered and discussed. The presented figures for an upper bound on network access speed are discussed and perspectives on further research presented.

A Method for Upper Bounding Long Term Growth of Network Access Speed

DEFF Research Database (Denmark)

Knudsen, Thomas Phillip; Pedersen, Jens Myrup; Madsen, Ole Brun

2004-01-01

The development in home Internet access speed has shown an exponential development with growth rates averaging 25% per year. For resource management in network provisioning it becomes an urgent question how long such growth can continue. This paper presents a method for calculating an upper bound...... to visual content driven growth, proceeding from datarate requirements for a full virtual environment. Scenarios and approaches for reducing datarate requirements are considered and discussed. The presented figures for an upper bound on network access speed are discussed and perspectives on further research...

A Cloud-Computing-Based Data Placement Strategy in High-Speed Railway

Directory of Open Access Journals (Sweden)

Hanning Wang

2012-01-01

Full Text Available As an important component of China’s transportation data sharing system, high-speed railway data sharing is a typical application of data-intensive computing. Currently, most high-speed railway data is shared in cloud computing environment. Thus, there is an urgent need for an effective cloud-computing-based data placement strategy in high-speed railway. In this paper, a new data placement strategy named hierarchical structure data placement strategy is proposed. The proposed method combines the semidefinite programming algorithm with the dynamic interval mapping algorithm. The semi-definite programming algorithm is suitable for the placement of files with various replications, ensuring that different replications of a file are placed on different storage devices, while the dynamic interval mapping algorithm ensures better self-adaptability of the data storage system. A hierarchical data placement strategy is proposed for large-scale networks. In this paper, a new theoretical analysis is provided, which is put in comparison with several other previous data placement approaches, showing the efficacy of the new analysis in several experiments.

Math Model and Calculation Analysis of Inter-harmonic of Double PWM Speed Control System in Distribution Network

Directory of Open Access Journals (Sweden)

Yang Wen-Huan

2014-11-01

Full Text Available Aiming at the problem that the distribution network voltage will fluctuate because of the inter-harmonic currents injected into the network by double PWM speed control system when regulating the speed of the asynchronous motor, we established the inter-harmonic current math model of double PWM speed control system according to switching function based on a real bridge crane. The distribution law of the inter-harmonic is got by calculating the grid-side currents and their spectrum while letting the motor run at different quadrants and frequencies. The result which is verified by simulation and experiment shows that the content of the inter harmonic currents is more than that of harmonic currents in double PWM speed control system, the frequency of the inter harmonics of the grid-side current mainly focus on the scope lower than the fundamental frequency, and when the motor runs at low frequencies, the THD of the grid-side current is high. The result has verified the reason why the voltage of a bridge crane distribution system of a deepwater port in Shanghai flickers.

ATM Quality of Service Tests for Digitized Video Using ATM Over Satellite: Laboratory Tests

Science.gov (United States)

Ivancic, William D.; Brooks, David E.; Frantz, Brian D.

1997-01-01

A digitized video application was used to help determine minimum quality of service parameters for asynchronous transfer mode (ATM) over satellite. For these tests, binomially distributed and other errors were digitally inserted in an intermediate frequency link via a satellite modem and a commercial gaussian noise generator. In this paper, the relation- ship between the ATM cell error and cell loss parameter specifications is discussed with regard to this application. In addition, the video-encoding algorithms, test configurations, and results are presented in detail.

MicroRNA203a suppresses glioma tumorigenesis through an ATM-dependent interferon response pathway.

Science.gov (United States)

Yang, Chuan He; Wang, Yinan; Sims, Michelle; Cai, Chun; He, Ping; Häcker, Hans; Yue, Junming; Cheng, Jinjun; Boop, Frederick A; Pfeffer, Lawrence M

2017-12-22

Glioblastoma (GBM) is a deadly and incurable brain tumor. Although microRNAs (miRNAs) play critical roles in regulating the cancer cell phenotype, the underlying mechanisms of how they regulate tumorigenesis are incompletely understood. We previously showed that miR-203a is expressed at relatively low levels in GBM patients, and ectopic miR-203a expression in GBM cell lines inhibited cell proliferation and migration, increased sensitivity to apoptosis induced by interferon (IFN) or temozolomide in vitro , and inhibited GBM tumorigenesis in vivo . Here we show that ectopic expression of miR-203a in GBM cell lines promotes the IFN response pathway as evidenced by increased IFN production and IFN-stimulated gene (ISG) expression, and high basal tyrosine phosphorylation of multiple STAT proteins. Importantly, we identified that miR-203a directly suppressed the protein levels of ataxia-telangiectasia mutated (ATM) kinase that negatively regulates IFN production. We found that high ATM expression in GBM correlates with poor patient survival and that ATM expression is inversely correlated with miR-203a expression. Knockout of ATM expression and inhibition of ATM function in GBM cell lines inhibited cell proliferation and migration, increased sensitivity to apoptosis induced by therapeutic agents in vitro , and markedly suppressed GBM tumor growth and promoted animal survival. In contrast, restoring ATM levels in GBM cells ectopically expressing miR-203a increased tumorigenicity and decreased animal survival. Our study suggests that low miR-203a expression in GBM suppresses the interferon response through an ATM-dependent pathway.

High-Speed Rail and Local Land Development : Case Studies in London and Las Vegas

Science.gov (United States)

2017-11-01

The efficacy of a high-speed rail system depends, in part, upon locating rail stations close to urban centers and integrating them into broader transportation networks and the urban realm. In addition, through economies of agglomeration, successful h...

ATM and p53 combined analysis predicts survival in glioblastoma multiforme patients: A clinicopathologic study.

Science.gov (United States)

Romano, Francesco Jacopo; Guadagno, Elia; Solari, Domenico; Borrelli, Giorgio; Pignatiello, Sara; Cappabianca, Paolo; Del Basso De Caro, Marialaura

2018-06-01

Glioblastoma is one of the most malignant cancers, with a distinguishing dismal prognosis: surgery followed by chemo- and radiotherapy represents the current standard of care, and chemo- and radioresistance underlie disease recurrence and short overall survival of patients suffering from this malignancy. ATM is a kinase activated by autophosphorylation upon DNA doublestrand breaks arising from errors during replication, byproducts of metabolism, chemotherapy or ionizing radiations; TP53 is one of the most popular tumor suppressor, with a preeminent role in DNA damage response and repair. To study the effects of the immunohistochemical expression of p-ATM and p53 in glioblastoma patients, 21 cases were retrospectively examined. In normal brain tissue, p-ATM was expressed only in neurons; conversely, in tumors cells, the protein showed a variable cytoplasmic expression (score: +,++,+++), with being completely undetectable in three cases. Statistical analysis revealed that high p-ATM score (++/+++) strongly correlated to shorter survival (P = 0.022). No difference in overall survival was registered between p53 normally expressed (NE) and overexpressed (OE) glioblastoma patients (P = 0.669). Survival analysis performed on the results from combined assessment of the two proteins showed that patients with NE p53 /low pATM score had longer overall survival than the NE p53/ high pATM score counterpart. Cox-regression analysis confirmed this finding (HR = 0.025; CI 95% = 0.002-0.284; P = 0.003). Our study outlined the immunohistochemical expression of p-ATM/p53 in glioblastomas and provided data on their possible prognostic/predictive of response role. A "non-oncogene addiction" to ATM for NEp53 glioblastoma could be postulated, strengthening the rationale for development of ATM inhibiting drugs. © 2018 Wiley Periodicals, Inc.

High speed heterostructure devices

CERN Document Server

Beer, Albert C; Willardson, R K; Kiehl, Richard A; Sollner, T C L Gerhard

1994-01-01

Volume 41 includes an in-depth review of the most important, high-speed switches made with heterojunction technology. This volume is aimed at the graduate student or working researcher who needs a broad overview andan introduction to current literature. Key Features * The first complete review of InP-based HFETs and complementary HFETs, which promise very low power and high speed * Offers a complete, three-chapter review of resonant tunneling * Provides an emphasis on circuits as well as devices.

Impedance Discontinuity Reduction Between High-Speed Differential Connectors and PCB Interfaces

Science.gov (United States)

Navidi, Sal; Agdinaoay, Rodell; Walter, Keith

2013-01-01

High-speed serial communication (i.e., Gigabit Ethernet) requires differential transmission and controlled impedances. Impedance control is essential throughout cabling, connector, and circuit board construction. An impedance discontinuity arises at the interface of a high-speed quadrax and twinax connectors and the attached printed circuit board (PCB). This discontinuity usually is lower impedance since the relative dielectric constant of the board is higher (i.e., polyimide approx. = 4) than the connector (Teflon approx. = 2.25). The discontinuity can be observed in transmit or receive eye diagrams, and can reduce the effective link margin of serial data networks. High-speed serial data network transmission improvements can be made at the connector-to-board interfaces as well as improving differential via hole impedances. The impedance discontinuity was improved by 10 percent by drilling a 20-mil (approx. = 0.5-mm) hole in between the pin of a differential connector spaced 55 mils (approx. = 1.4 mm) apart as it is attached to the PCB. The effective dielectric constant of the board can be lowered by drilling holes into the board material between the differential lines in a quadrax or twinax connector attachment points. The differential impedance is inversely proportional to the square root of the relative dielectric constant. This increases the differential impedance and thus reduces the above described impedance discontinuity. The differential via hole impedance can also be increased in the same manner. This technique can be extended to multiple smaller drilled holes as well as tapered holes (i.e., big in the middle followed by smaller ones diagonally).

ATM Coastal Topography-Texas, 2001: UTM Zone 14

Science.gov (United States)

Klipp, Emily S.; Nayegandhi, Amar; Brock, John C.; Sallenger, A.H.; Bonisteel, Jamie M.; Yates, Xan; Wright, C. Wayne

2009-01-01

These remotely sensed, geographically referenced elevation measurements of lidar-derived first-surface (FS) topography were produced collaboratively by the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL, and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of a portion of the Texas coastline within UTM zone 14, acquired October 12-13, 2001. The datasets are made available for use as a management tool to research scientists and natural-resource managers. An innovative scanning lidar instrument originally developed by NASA, and known as the Airborne Topographic Mapper (ATM), was used during data acquisition. The ATM system is a scanning lidar system that measures high-resolution topography of the land surface and incorporates a green-wavelength laser operating at pulse rates of 2 to 10 kilohertz. Measurements from the laser-ranging device are coupled with data acquired from inertial navigation system (INS) attitude sensors and differentially corrected global positioning system (GPS) receivers to measure topography of the surface at accuracies of +/-15 centimeters. The nominal ATM platform is a Twin Otter or P-3 Orion aircraft, but the instrument may be deployed on a range of light aircraft. Elevation measurements were collected over the survey area using the ATM system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of lidar data in an interactive or batch mode. Modules for presurvey flight-line definition, flight-path plotting, lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant return within each waveform. ALPS is used

Premeiotic germ cell defect in seminiferous tubules of Atm-null testis

International Nuclear Information System (INIS)

Takubo, Keiyo; Hirao, Atsushi; Ohmura, Masako; Azuma, Masaki; Arai, Fumio; Nagamatsu, Go; Suda, Toshio

2006-01-01

Lifelong spermatogenesis is maintained by coordinated sequential processes including self-renewal of stem cells, proliferation of spermatogonial cells, meiotic division, and spermiogenesis. It has been shown that ataxia telangiectasia-mutated (ATM) is required for meiotic division of the seminiferous tubules. Here, we show that, in addition to its role in meiosis, ATM has a pivotal role in premeiotic germ cell maintenance. ATM is activated in premeiotic spermatogonial cells and the Atm-null testis shows progressive degeneration. In Atm-null testicular cells, differing from bone marrow cells of Atm-null mice, reactive oxygen species-mediated p16 Ink4a activation does not occur in Atm-null premeiotic germ cells, which suggests the involvement of different signaling pathways from bone marrow defects. Although Atm-null bone marrow undergoes p16 Ink4a -mediated cellular senescence program, Atm-null premeiotic germ cells exhibited cell cycle arrest and apoptotic elimination of premeiotic germ cells, which is different from p16 Ink4a -mediated senescence

Tyrosine 370 phosphorylation of ATM positively regulates DNA damage response

Science.gov (United States)

Lee, Hong-Jen; Lan, Li; Peng, Guang; Chang, Wei-Chao; Hsu, Ming-Chuan; Wang, Ying-Nai; Cheng, Chien-Chia; Wei, Leizhen; Nakajima, Satoshi; Chang, Shih-Shin; Liao, Hsin-Wei; Chen, Chung-Hsuan; Lavin, Martin; Ang, K Kian; Lin, Shiaw-Yih; Hung, Mien-Chie

2015-01-01

Ataxia telangiectasia mutated (ATM) mediates DNA damage response by controling irradiation-induced foci formation, cell cycle checkpoint, and apoptosis. However, how upstream signaling regulates ATM is not completely understood. Here, we show that upon irradiation stimulation, ATM associates with and is phosphorylated by epidermal growth factor receptor (EGFR) at Tyr370 (Y370) at the site of DNA double-strand breaks. Depletion of endogenous EGFR impairs ATM-mediated foci formation, homologous recombination, and DNA repair. Moreover, pretreatment with an EGFR kinase inhibitor, gefitinib, blocks EGFR and ATM association, hinders CHK2 activation and subsequent foci formation, and increases radiosensitivity. Thus, we reveal a critical mechanism by which EGFR directly regulates ATM activation in DNA damage response, and our results suggest that the status of ATM Y370 phosphorylation has the potential to serve as a biomarker to stratify patients for either radiotherapy alone or in combination with EGFR inhibition. PMID:25601159

ATM function and its relationship with ATM gene mutations in chronic lymphocytic leukemia with the recurrent deletion (11q22.3-23.2).

Science.gov (United States)

Jiang, Y; Chen, H-C; Su, X; Thompson, P A; Liu, X; Do, K-A; Wierda, W; Keating, M J; Plunkett, W

2016-09-02

Approximately 10-20% of chronic lymphocytic leukemia (CLL) patients exhibit del(11q22-23) before treatment, this cohort increases to over 40% upon progression following chemoimmunotherapy. The coding sequence of the DNA damage response gene, ataxia-telangiectasia-mutated (ATM), is contained in this deletion. The residual ATM allele is frequently mutated, suggesting a relationship between gene function and clinical response. To investigate this possibility, we sought to develop and validate an assay for the function of ATM protein in these patients. SMC1 (structural maintenance of chromosomes 1) and KAP1 (KRAB-associated protein 1) were found to be unique substrates of ATM kinase by immunoblot detection following ionizing radiation. Using a pool of eight fluorescence in situ hybridization-negative CLL samples as a standard, the phosphorylation of SMC1 and KAP1 from 46 del (11q22-23) samples was analyzed using normal mixture model-based clustering. This identified 13 samples (28%) that were deficient in ATM function. Targeted sequencing of the ATM gene of these samples, with reference to genomic DNA, revealed 12 somatic mutations and 15 germline mutations in these samples. No strong correlation was observed between ATM mutation and function. Therefore, mutation status may not be taken as an indicator of ATM function. Rather, a direct assay of the kinase activity should be used in the development of therapies.

Effects of high-speed homogenization and high-pressure homogenization on structure of tomato residue fibers.

Science.gov (United States)

Hua, Xiao; Xu, Shanan; Wang, Mingming; Chen, Ying; Yang, Hui; Yang, Ruijin

2017-10-01

Tomato residue fibers obtained after derosination and deproteinization were processed by high-speed homogenization (HSH) and high-pressure homogenization (HPH), and their effects on fiber structure was investigated, respectively. Characterizations including particle size distribution, SEM, TEM and XRD were performed. HSH could break raw fibers to small particles of around 60μm, while HPH could reshape fibers to build network structure. Microfibrils were released and their nanostructure consisting of elementary fibrils was observed by TEM. XRD patterns indicated both HSH and HPH could hardly alter the nanostructure of the fibers. Physicochemical properties including expansibility, WHC and OHC were determined. Both HSH and HPH could increase the soluble fiber content by about 8%, but HSH-HPH combined processing did not show better result. Acid (4mol/L HCl) was used in replacement of water medium and the acidic degradation of fibers could be promoted by high speed shearing or high pressure processing. Copyright © 2017 Elsevier Ltd. All rights reserved.

Aircraft Emission Inventories Projected in Year 2015 for a High Speed Civil Transport (HSCT) Universal Airline Network. Final report

Energy Technology Data Exchange (ETDEWEB)

Baughcum, S.L.; Henderson, S.C.

1995-07-01

This report describes the development of a three-dimensional database of aircraft fuel burn and emissions (fuel burned, NOx, CO, and hydrocarbons) from projected fleets of high speed civil transports (HSCT`s) on a universal airline network. Inventories for 500 and 1000 HSCT fleets, as well as the concurrent subsonic fleets, were calculated. The objective of this work was to evaluate the changes in geographical distribution of the HSCT emissions as the fleet size grew from 500 to 1000 HSCT`s. For this work, a new expanded HSCT network was used and flights projected using a market penetration analysis rather than assuming equal penetration as was done in the earlier studies. Emission inventories on this network were calculated for both Mach 2.0 and Mach 2.4 HSCT fleets with NOx cruise emission indices of approximately 5 and 15 grams NOx/kg fuel. These emissions inventories are available for use by atmospheric scientists conducting the Atmospheric Effects of Stratospheric Aircraft (AESA) modeling studies. Fuel burned and emissions of nitrogen oxides (NOx as NO2), carbon monoxide, and hydrocarbons have been calculated on a 1 degree latitude x 1 degree longitude x 1 kilometer attitude grid and delivered to NASA as electronic files.

Florida intercity high speed rail passenger service

Energy Technology Data Exchange (ETDEWEB)

Najafi, F.T.; Watford, S.; Moore, G.; Des, A. [Florida Univ., Gainesville, FL (United States). Dept. of Civil Engineering

1997-10-01

Plans for a new high-speed rail (HSR) transportation system in Florida were reviewed. HSR is believed to be the least expensive, most energy-efficient and least environmentally harmful alternative to air and highway travel. The system in Florida will be used as a case study to determine its overall impact on the environment, people and economy. The 300-plus mile system will move travelers at speeds of over 200 mph between Miami, Orlando, and Tampa. The study will identify the impacts of a HSR system on existing transportation networks, environment, energy, growth and growth distribution, safety, economy, travel time, and tourism. Transportation problems and the innovative mechanisms needed to realize the joint public and private venture approach to planning, locating, permitting, managing, financing, constructing and maintaining an inter-regional HSR line for the state were studied. The all-electric train would greatly help the environment in two ways: (1) zero emissions from the train itself, and (2) the reduction of trips by automobile and aircraft would reduce the amount of fuel and energy being used. 4 refs., 1 fig.

STEADY STATE PERFORMANCES ANALYSIS OF MODERN MARINE TWO-STROKE LOW SPEED DIESEL ENGINE USING MLP NEURAL NETWORK MODEL

Directory of Open Access Journals (Sweden)

Ozren Bukovac

2016-01-01

Full Text Available Compared to the other marine engines for ship propulsion, turbocharged two-stroke low speed diesel engines have advantages due to their high efficiency and reliability. Modern low speed ”intelligent” marine diesel engines have a flexibility in its operation due to the variable fuel injection strategy and management of the exhaust valve drive. This paper carried out verified zerodimensional numerical simulations which have been used for MLP (Multilayer Perceptron neural network predictions of marine two-stroke low speed diesel engine steady state performances. The developed MLP neural network was used for marine engine optimized operation control. The paper presents an example of achieving lowest specific fuel consumption and for minimization of the cylinder process highest temperature for reducing NOx emission. Also, the developed neural network was used to achieve optimal exhaust gases heat flow for utilization. The obtained data maps give insight into the optimal working areas of simulated marine diesel engine, depending on the selected start of the fuel injection (SOI and the time of the exhaust valve opening (EVO.

High Speed Rail commuting: Efficiency analysis of the Spanish HSR links

Energy Technology Data Exchange (ETDEWEB)

Moyano, A.

2016-07-01

This paper is centred on the analysis of the commuting High Speed Rail (HSR) links that are possible nowadays in the Spanish network. The large development of the network multiplies the possibilities of travelling for commuting. However, not only the feasibility but also the characteristics of the trip, in terms of ticket cost and time spent on the travel, become essential factors when considering HSR commuting links. This paper presents a person-based methodology focused on travellers’ needs and working schedules constraints, which allow differentiating among connections and identifying those intervals of time spent and costs that are affordable for commuting. (Author)

Low-latency video transmission over high-speed WPANs based on low-power video compression

DEFF Research Database (Denmark)

Belyaev, Evgeny; Turlikov, Andrey; Ukhanova, Ann

2010-01-01

This paper presents latency-constrained video transmission over high-speed wireless personal area networks (WPANs). Low-power video compression is proposed as an alternative to uncompressed video transmission. A video source rate control based on MINMAX quality criteria is introduced. Practical...

Absence of Wip1 partially rescues Atm deficiency phenotypes in mice

Science.gov (United States)

Darlington, Yolanda; Nguyen, Thuy-Ai; Moon, Sung-Hwan; Herron, Alan; Rao, Pulivarthi; Zhu, Chengming; Lu, Xiongbin; Donehower, Lawrence A.

2011-01-01

Wildtype p53-Induced Phosphatase 1 (WIP1) is a serine/threonine phosphatase that dephosphorylates proteins in the ataxia telangiectasia mutated (ATM)-initiated DNA damage response pathway. WIP1 may play a homeostatic role in ATM signaling by returning the cell to a normal pre-stress state following completion of DNA repair. To better understand the effects of WIP1 on ATM signaling, we crossed Atm-deficient mice to Wip1-deficient mice and characterized phenotypes of the double knockout progeny. We hypothesized that the absence of Wip1 might rescue Atm deficiency phenotypes. Atm null mice, like ATM-deficient humans with the inherited syndrome ataxia telangiectasia, exhibit radiation sensitivity, fertility defects, and are T-cell lymphoma prone. Most double knockout mice were largely protected from lymphoma development and had a greatly extended lifespan compared to Atm null mice. Double knockout mice had increased p53 and H2AX phosphorylation and p21 expression compared to their Atm null counterparts, indicating enhanced p53 and DNA damage responses. Additionally, double knockout splenocytes displayed reduced chromosomal instability compared to Atm null mice. Finally, doubly null mice were partially rescued from infertility defects observed in Atm null mice. These results indicate that inhibition of WIP1 may represent a useful strategy for cancer treatment in general and A-T patients in particular. PMID:21765465

Using neural networks to speed up optimization algorithms

CERN Document Server

Bazan, M

2000-01-01

The paper presents the application of radial-basis-function (RBF) neural networks to speed up deterministic search algorithms used for the design and optimization of superconducting LHC magnets. The optimization of the iron yoke of the main dipoles requires a number of numerical field computations per trial solution as the field quality depends on the excitation of the magnets. This results in computation times of about 30 minutes for each objective function evaluation (on a DEC-Alpha 600/333) and only the most robust (deterministic) optimization algorithms can be applied. Using a RBF function approximator, the achieved speed-up of the search algorithm is in the order of 25% for problems with two parameters and about 18% for problems with three and five design variables. (13 refs).

Modeling and Speed Control of Induction Motor Drives Using Neural Networks

Directory of Open Access Journals (Sweden)

V. Jamuna

2010-08-01

Full Text Available Speed control of induction motor drives using neural networks is presented. The mathematical model of single phase induction motor is developed. A new simulink model for a neural network-controlled bidirectional chopper fed single phase induction motor is proposed. Under normal operation, the true drive parameters are real-time identified and they are converted into the controller parameters through multilayer forward computation by neural networks. Comparative study has been made between the conventional and neural network controllers. It is observed that the neural network controlled drive system has better dynamic performance, reduced overshoot and faster transient response than the conventional controlled system.

Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry.

Science.gov (United States)

Graham, Mark E; Lavin, Martin F; Kozlov, Sergei V

2017-01-01

ATM (ataxia-telangiectasia mutated) protein kinase is a key regulator of cellular responses to DNA damage and oxidative stress. DNA damage triggers complex cascade of signaling events leading to numerous posttranslational modification on multitude of proteins. Understanding the regulation of ATM kinase is therefore critical not only for understanding the human genetic disorder ataxia-telangiectasia and potential treatment strategies, but essential for deciphering physiological responses of cells to stress. These responses play an important role in carcinogenesis, neurodegeneration, and aging. We focus here on the identification of DNA damage inducible ATM phosphorylation sites to understand the importance of autophosphorylation in the mechanism of ATM kinase activation. We demonstrate the utility of using immunoprecipitated ATM in quantitative LC-MS/MS workflow with stable isotope dimethyl labeling of ATM peptides for identification of phosphorylation sites.

Prevalence of deleterious ATM germline mutations in gastric cancer patients.

Science.gov (United States)

Huang, Dong-Sheng; Tao, Hou-Quan; He, Xu-Jun; Long, Ming; Yu, Sheng; Xia, Ying-Jie; Wei, Zhang; Xiong, Zikai; Jones, Sian; He, Yiping; Yan, Hai; Wang, Xiaoyue

2015-12-01

Besides CDH1, few hereditary gastric cancer predisposition genes have been previously reported. In this study, we discovered two germline ATM mutations (p.Y1203fs and p.N1223S) in a Chinese family with a history of gastric cancer by screening 83 cancer susceptibility genes. Using a published exome sequencing dataset, we found deleterious germline mutations of ATM in 2.7% of 335 gastric cancer patients of different ethnic origins. The frequency of deleterious ATM mutations in gastric cancer patients is significantly higher than that in general population (p=0.0000435), suggesting an association of ATM mutations with gastric cancer predisposition. We also observed biallelic inactivation of ATM in tumors of two gastric cancer patients. Further evaluation of ATM mutations in hereditary gastric cancer will facilitate genetic testing and risk assessment.

Downregulation of ATM Gene and Protein Expression in Canine Mammary Tumors.

Science.gov (United States)

Raposo-Ferreira, T M M; Bueno, R C; Terra, E M; Avante, M L; Tinucci-Costa, M; Carvalho, M; Cassali, G D; Linde, S D; Rogatto, S R; Laufer-Amorim, R

2016-11-01

The ataxia telangiectasia mutated (ATM) gene encodes a protein associated with DNA damage repair and maintenance of genomic integrity. In women, ATM transcript and protein downregulation have been reported in sporadic breast carcinomas, and the absence of ATM protein expression has been associated with poor prognosis. The aim of this study was to evaluate ATM gene and protein expression in canine mammary tumors and their association with clinical outcome. ATM gene and protein expression was evaluated by reverse transcription-quantitative polymerase chain reaction and immunohistochemistry, respectively, in normal mammary gland samples (n = 10), benign mammary tumors (n = 11), nonmetastatic mammary carcinomas (n = 19), and metastatic mammary carcinomas (n = 11). Lower ATM transcript levels were detected in benign mammary tumors and carcinomas compared with normal mammary glands (P = .011). Similarly, lower ATM protein expression was observed in benign tumors (P = .0003), nonmetastatic mammary carcinomas (P ATM gene or protein levels were detected among benign tumors and nonmetastatic and metastatic mammary carcinomas (P > .05). The levels of ATM gene or protein expression were not significantly associated with clinical and pathological features or with survival. Similar to human breast cancer, the data in this study suggest that ATM gene and protein downregulation is involved in canine mammary gland tumorigenesis. © The Author(s) 2016.

14 CFR 23.253 - High speed characteristics.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false High speed characteristics. 23.253 Section... Requirements § 23.253 High speed characteristics. If a maximum operating speed VMO/MMO is established under § 23.1505(c), the following speed increase and recovery characteristics must be met: (a) Operating...

SPEED: A Stateless Protocol for Real-Time Communication in Sensor Networks

National Research Council Canada - National Science Library

He, Tian; Stankovic, John A; Lu, Chenyang; Abdelzaher, Tarek

2003-01-01

.... End-to-end soft real-time communication is achieved by maintaining a desired delivery speed across the sensor network through a novel combination of feedback control and non-deterministic geographic forwarding...

A novel ATM-dependent checkpoint defect distinct from loss of function mutation promotes genomic instability in melanoma.

Science.gov (United States)

Spoerri, Loredana; Brooks, Kelly; Chia, KeeMing; Grossman, Gavriel; Ellis, Jonathan J; Dahmer-Heath, Mareike; Škalamera, Dubravka; Pavey, Sandra; Burmeister, Bryan; Gabrielli, Brian

2016-05-01

Melanomas have high levels of genomic instability that can contribute to poor disease prognosis. Here, we report a novel defect of the ATM-dependent cell cycle checkpoint in melanoma cell lines that promotes genomic instability. In defective cells, ATM signalling to CHK2 is intact, but the cells are unable to maintain the cell cycle arrest due to elevated PLK1 driving recovery from the arrest. Reducing PLK1 activity recovered the ATM-dependent checkpoint arrest, and over-expressing PLK1 was sufficient to overcome the checkpoint arrest and increase genomic instability. Loss of the ATM-dependent checkpoint did not affect sensitivity to ionizing radiation demonstrating that this defect is distinct from ATM loss of function mutations. The checkpoint defective melanoma cell lines over-express PLK1, and a significant proportion of melanomas have high levels of PLK1 over-expression suggesting this defect is a common feature of melanomas. The inability of ATM to impose a cell cycle arrest in response to DNA damage increases genomic instability. This work also suggests that the ATM-dependent checkpoint arrest is likely to be defective in a higher proportion of cancers than previously expected. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Mobile networks architecture

CERN Document Server

Perez, Andre

2013-01-01

This book explains the evolutions of architecture for mobiles and summarizes the different technologies:- 2G: the GSM (Global System for Mobile) network, the GPRS (General Packet Radio Service) network and the EDGE (Enhanced Data for Global Evolution) evolution;- 3G: the UMTS (Universal Mobile Telecommunications System) network and the HSPA (High Speed Packet Access) evolutions:- HSDPA (High Speed Downlink Packet Access),- HSUPA (High Speed Uplink Packet Access),- HSPA+;- 4G: the EPS (Evolved Packet System) network.The telephone service and data transmission are the

Mobility Performance in Slow- and High-Speed LTE Real Scenarios

DEFF Research Database (Denmark)

Gimenez, Lucas Chavarria; Cascino, Maria Carmela; Stefan, Maria

2016-01-01

Mobility performance and handover data interruption times in real scenarios are studied by means of field measurements in an operational LTE network. Both slow- and high-speed scenarios are analyzed by collecting results from two different areas: Aalborg downtown and the highway which encircles...... in the city center as cells on the same site often cover different non-crossing street canyons. Moreover, no handover failures are experienced in the measurements which confirms robust LTE mobility performance. The average interruption time, which is at least equal to the handover execution time, lays within...... the same city. Measurements reveal that the terminal is configured by the network with different handover parametrization depending on the serving cell, which indicates the use of mobility robustness optimization. Although the network is dominated by three sector sites, no intra-site handovers are observed...

ATM facilitates mouse gammaherpesvirus reactivation from myeloid cells during chronic infection.

Science.gov (United States)

Kulinski, Joseph M; Darrah, Eric J; Broniowska, Katarzyna A; Mboko, Wadzanai P; Mounce, Bryan C; Malherbe, Laurent P; Corbett, John A; Gauld, Stephen B; Tarakanova, Vera L

2015-09-01

Gammaherpesviruses are cancer-associated pathogens that establish life-long infection in most adults. Insufficiency of Ataxia-Telangiectasia mutated (ATM) kinase leads to a poor control of chronic gammaherpesvirus infection via an unknown mechanism that likely involves a suboptimal antiviral response. In contrast to the phenotype in the intact host, ATM facilitates gammaherpesvirus reactivation and replication in vitro. We hypothesized that ATM mediates both pro- and antiviral activities to regulate chronic gammaherpesvirus infection in an immunocompetent host. To test the proposed proviral activity of ATM in vivo, we generated mice with ATM deficiency limited to myeloid cells. Myeloid-specific ATM deficiency attenuated gammaherpesvirus infection during the establishment of viral latency. The results of our study uncover a proviral role of ATM in the context of gammaherpesvirus infection in vivo and support a model where ATM combines pro- and antiviral functions to facilitate both gammaherpesvirus-specific T cell immune response and viral reactivation in vivo. Copyright © 2015 Elsevier Inc. All rights reserved.

Drosophila atm/telomere fusion is required for telomeric localization of HP1 and telomere position effect.

Science.gov (United States)

Oikemus, Sarah R; McGinnis, Nadine; Queiroz-Machado, Joana; Tukachinsky, Hanna; Takada, Saeko; Sunkel, Claudio E; Brodsky, Michael H

2004-08-01

Terminal deletions of Drosophila chromosomes can be stably protected from end-to-end fusion despite the absence of all telomere-associated sequences. The sequence-independent protection of these telomeres suggests that recognition of chromosome ends might contribute to the epigenetic protection of telomeres. In mammals, Ataxia Telangiectasia Mutated (ATM) is activated by DNA damage and acts through an unknown, telomerase-independent mechanism to regulate telomere length and protection. We demonstrate that the Drosophila homolog of ATM is encoded by the telomere fusion (tefu) gene. In the absence of ATM, telomere fusions occur even though telomere-specific Het-A sequences are still present. High levels of spontaneous apoptosis are observed in ATM-deficient tissues, indicating that telomere dysfunction induces apoptosis in Drosophila. Suppression of this apoptosis by p53 mutations suggests that loss of ATM activates apoptosis through a DNA damage-response mechanism. Loss of ATM reduces the levels of heterochromatin protein 1 (HP1) at telomeres and suppresses telomere position effect. We propose that recognition of chromosome ends by ATM prevents telomere fusion and apoptosis by recruiting chromatin-modifying complexes to telomeres.

Automated Transportation Management System (ATMS) Configuration Management Plan. Revision 1

International Nuclear Information System (INIS)

Weidert, R.S.

1994-01-01

This document describes the Software Configuration Management (SCM) approach and procedures to be utilized in developing and maintaining the Automated Transportation Management System (ATMS). The configuration management procedures are necessary to ensure that any changes made to software and related documentation are consistent with ATMS goals and contained securely in a central library. This plan applies to all software and associated documentation used in producing ATMS V1.0 and ATMS V2.0 system

High-Speed Photography

International Nuclear Information System (INIS)

Paisley, D.L.; Schelev, M.Y.

1998-01-01

The applications of high-speed photography to a diverse set of subjects including inertial confinement fusion, laser surgical procedures, communications, automotive airbags, lightning etc. are briefly discussed. (AIP) copyright 1998 Society of Photo-Optical Instrumentation Engineers

A NEW METHOD TO DETECT REGIONS ENDANGERED BY HIGH WIND SPEEDS

Directory of Open Access Journals (Sweden)

P. Fischer

2016-06-01

Full Text Available In this study we evaluate whether the methodology of Boosted Regression Trees (BRT suits for accurately predicting maximum wind speeds. As predictors a broad set of parameters derived from a Digital Elevation Model (DEM acquired within the Shuttle Radar Topography Mission (SRTM is used. The derived parameters describe the surface by means of quantities (e.g. slope, aspect and quality (landform classification. Furthermore land cover data from the CORINE dataset is added. The response variable is maximum wind speed, measurements are provided by a network of weather stations. The area of interest is Switzerland, a country which suits perfectly for this study because of its highly dynamic orography and various landforms.

Fabrication and characterization of MCC approved testing material - ATM-12 glass

International Nuclear Information System (INIS)

Wald, J.W.

1985-10-01

The Materials Characterization Center (MCC) Approved Testing Material ATM-12 is a borosilicate glass that incorporates elements typical of high-level waste (HLW) resulting from the reprocessing of commercial nuclear reactor fuels. The composition has been adjusted to match that predicted for HLW type 76-68 glass at an age of 300 y. Radioactive constituents contained in this glass include depleted uranium, 99 Tc, 237 Np, 239 Pu, and 241 Am. The glass was produced by the MCC at the Pacific Northwest Laboratory (PNL). ATM-12 glass ws produced from July to November of 1984 at the request of the Nevada Nuclear Waste Site Investigations (NNWSI) Program and is the third in a series of glasses produced for NNWSI. Most of the glass produced was in the form of cast bars; special castings and crushed material were also produced. Three kilograms of ATM-12 glass were produced from a feedstock melted in a nitrogen-atmosphere glove box at 1150 0 C in a platinum crucible, and formed into stress-annealed rectangular bars and the special casting shapes requested by NNWSI. Bars of ATM-12 were nominally 1.9 x 1.9 x 10 cm, with an average mass of 111 g each. Nineteen bars and 37 special castings were made. ATM-12 glass has been provided to the NNWSI Program, in the form of bars, crushed powder and special castings. As of August 1985 approximately 590 g of ATM-12 is available for distribution. Requests for materials or services related to this glass should be directed to the Materials Characterization Center Program Office, PNL

A current review of high speed railways experiences in Asia and Europe

Science.gov (United States)

Purba, Aleksander; Nakamura, Fumihiko; Dwsbu, Chatarina Niken; Jafri, Muhammad; Pratomo, Priyo

2017-11-01

High-Speed Railways (HSR) is currently regarded as one of the most significant technological breakthroughs in passenger transportation developed in the second half of the 20th century. At the beginning of 2008, there were about 10,000 kilometers of new high-speed lines in operation in Asia and Europe regions to provide high-speed services to passengers willing to pay for lower travel time and quality improvement in rail transport. And since 2010, HSR itself has received a great deal of attention in Indonesia. Some transportation analysts contend that Indonesia, particularly Java and Sumatera islands need a high-speed rail network to be economically competitive with countries in Asia and Europe. On April 2016, Indonesia-China consortium Kereta Cepat Indonesia China (KCIC) signed an engineering, procurement, and construction contract to build the HSR with a consortium of seven companies called the High-Speed Railway Contractor Consortium. The HSR is expected to debut by May 2019, offering a 45-minute trip covering a roughly 150 km route. However, building, maintaining and operating HSR line is expensive; it involves a significant amount of sunk costs and may substantially compromise both the transport policy of a country and the development of its transport sector for decades. The main objective of this paper is to discuss some characteristics of the HSR services from an economic viewpoint, while simultaneously developing an empirical framework that should help us to understand, in more detail, the factors determining the success of the HSR as transport alternative based on current experiences of selected Asian and European countries.

ATM Technology and Banking System in West African Sub-Region ...

African Journals Online (AJOL)

User

2011-04-19

Apr 19, 2011 ... At this point in time, it is pertinent to ask, what is ATM? ATM is Automated .... can be installed on ATMs running Microsoft Windows XP operating system that records sensitive ... almost real time bank services. The citizens of ...

ATM down-regulation is associated with poor prognosis in sporadic breast carcinomas

DEFF Research Database (Denmark)

Bueno, R C; Canevari, R A; Villacis, R A R

2014-01-01

BACKGROUND: Ataxia telangiectasia-mutated (ATM) gene downexpression has been reported in sporadic breast carcinomas (BC); however, the prognostic value and mechanisms of ATM deregulation remain unclear. PATIENTS AND METHODS: ATM and miRNAs (miR-26a, miR-26b, miR-203, miR-421, miR-664, miR-576-5p...... and miR-18a) expression levels were evaluated by quantitative real-time PCR (RT-qPCR) in 52 BC and 3 normal breast samples. ATM protein expression was assessed by immunohistochemistry in 968 BC and 35 adjacent normal breast tissues. ATM copy number alteration was detected by array comparative genomic...... hybridization (aCGH) in 42 tumours. RESULTS: Low ATM levels were associated with tumour grade. Absence of ATM protein expression was associated with distant metastasis (P ATM...

Absence of ERK5/MAPK7 delays tumorigenesis in Atm-/- mice.

Science.gov (United States)

Granados-Jaén, Alba; Angulo-Ibáñez, Maria; Rovira-Clavé, Xavier; Gamez, Celina Paola Vasquez; Soriano, Francesc X; Reina, Manuel; Espel, Enric

2016-11-15

Ataxia-telangiectasia mutated (ATM) is a cell cycle checkpoint kinase that upon activation by DNA damage leads to cell cycle arrest and DNA repair or apoptosis. The absence of Atm or the occurrence of loss-of-function mutations in Atm predisposes to tumorigenesis. MAPK7 has been implicated in numerous types of cancer with pro-survival and pro-growth roles in tumor cells, but its functional relation with tumor suppressors is not clear. In this study, we show that absence of MAPK7 delays death due to spontaneous tumor development in Atm-/- mice. Compared with Atm-/- thymocytes, Mapk7-/-Atm-/- thymocytes exhibited an improved response to DNA damage (increased phosphorylation of H2AX) and a restored apoptotic response after treatment of mice with ionizing radiation. These findings define an antagonistic function of ATM and MAPK7 in the thymocyte response to DNA damage, and suggest that the lack of MAPK7 inhibits thymic lymphoma growth in Atm-/- mice by partially restoring the DNA damage response in thymocytes.

CHEETAH: circuit-switched high-speed end-to-end transport architecture

Science.gov (United States)

Veeraraghavan, Malathi; Zheng, Xuan; Lee, Hyuk; Gardner, M.; Feng, Wuchun

2003-10-01

Leveraging the dominance of Ethernet in LANs and SONET/SDH in MANs and WANs, we propose a service called CHEETAH (Circuit-switched High-speed End-to-End Transport ArcHitecture). The service concept is to provide end hosts with high-speed, end-to-end circuit connectivity on a call-by-call shared basis, where a "circuit" consists of Ethernet segments at the ends that are mapped into Ethernet-over-SONET long-distance circuits. This paper focuses on the file-transfer application for such circuits. For this application, the CHEETAH service is proposed as an add-on to the primary Internet access service already in place for enterprise hosts. This allows an end host that is sending a file to first attempt setting up an end-to-end Ethernet/EoS circuit, and if rejected, fall back to the TCP/IP path. If the circuit setup is successful, the end host will enjoy a much shorter file-transfer delay than on the TCP/IP path. To determine the conditions under which an end host with access to the CHEETAH service should attempt circuit setup, we analyze mean file-transfer delays as a function of call blocking probability in the circuit-switched network, probability of packet loss in the IP network, round-trip times, link rates, and so on.

High-speed noise-free optical quantum memory

Science.gov (United States)

Kaczmarek, K. T.; Ledingham, P. M.; Brecht, B.; Thomas, S. E.; Thekkadath, G. S.; Lazo-Arjona, O.; Munns, J. H. D.; Poem, E.; Feizpour, A.; Saunders, D. J.; Nunn, J.; Walmsley, I. A.

2018-04-01

Optical quantum memories are devices that store and recall quantum light and are vital to the realization of future photonic quantum networks. To date, much effort has been put into improving storage times and efficiencies of such devices to enable long-distance communications. However, less attention has been devoted to building quantum memories which add zero noise to the output. Even small additional noise can render the memory classical by destroying the fragile quantum signatures of the stored light. Therefore, noise performance is a critical parameter for all quantum memories. Here we introduce an intrinsically noise-free quantum memory protocol based on two-photon off-resonant cascaded absorption (ORCA). We demonstrate successful storage of GHz-bandwidth heralded single photons in a warm atomic vapor with no added noise, confirmed by the unaltered photon-number statistics upon recall. Our ORCA memory meets the stringent noise requirements for quantum memories while combining high-speed and room-temperature operation with technical simplicity, and therefore is immediately applicable to low-latency quantum networks.

Noncanonical ATM Activation and Signaling in Response to Transcription-Blocking DNA Damage.

Science.gov (United States)

Marteijn, Jurgen A; Vermeulen, Wim; Tresini, Maria

2017-01-01

Environmental genotoxins and metabolic byproducts generate DNA lesions that can cause genomic instability and disrupt tissue homeostasis. To ensure genomic integrity, cells employ mechanisms that convert signals generated by stochastic DNA damage into organized responses, including activation of repair systems, cell cycle checkpoints, and apoptotic mechanisms. DNA damage response (DDR) signaling pathways coordinate these responses and determine cellular fates in part, by transducing signals that modulate RNA metabolism. One of the master DDR coordinators, the Ataxia Telangiectasia Mutated (ATM) kinase, has a fundamental role in mediating DNA damage-induced changes in mRNA synthesis. ATM acts by modulating a variety of RNA metabolic pathways including nascent RNA splicing, a process catalyzed by the spliceosome. Interestingly, ATM and the spliceosome influence each other's activity in a reciprocal manner by a pathway that initiates when transcribing RNA polymerase II (RNAPII) encounters DNA lesions that prohibit forward translocation. In response to stalling of RNAPII assembly of late-stage spliceosomes is disrupted resulting in increased splicing factor mobility. Displacement of spliceosomes from lesion-arrested RNA polymerases facilitates formation of R-loops between the nascent RNA and DNA adjacent to the transcription bubble. R-loops signal for noncanonical ATM activation which in quiescent cells occurs in absence of detectable dsDNA breaks. In turn, activated ATM signals to regulate spliceosome dynamics and AS genome wide.This chapter describes the use of fluorescence microscopy methods that can be used to evaluate noncanonical ATM activation by transcription-blocking DNA damage. First, we present an immunofluorescence-detection method that can be used to evaluate ATM activation by autophosphorylation, in fixed cells. Second, we present a protocol for Fluorescence Recovery After Photobleaching (FRAP) of GFP-tagged splicing factors, a highly sensitive and

SEAL FOR HIGH SPEED CENTRIFUGE

Science.gov (United States)

Skarstrom, C.W.

1957-12-17

A seal is described for a high speed centrifuge wherein the centrifugal force of rotation acts on the gasket to form a tight seal. The cylindrical rotating bowl of the centrifuge contains a closure member resting on a shoulder in the bowl wall having a lower surface containing bands of gasket material, parallel and adjacent to the cylinder wall. As the centrifuge speed increases, centrifugal force acts on the bands of gasket material forcing them in to a sealing contact against the cylinder wall. This arrangememt forms a simple and effective seal for high speed centrifuges, replacing more costly methods such as welding a closure in place.

ATM directs DNA damage responses and proteostasis via genetically separable pathways.

Science.gov (United States)

Lee, Ji-Hoon; Mand, Michael R; Kao, Chung-Hsuan; Zhou, Yi; Ryu, Seung W; Richards, Alicia L; Coon, Joshua J; Paull, Tanya T

2018-01-09

The protein kinase ATM is a master regulator of the DNA damage response but also responds directly to oxidative stress. Loss of ATM causes ataxia telangiectasia, a neurodegenerative disorder with pleiotropic symptoms that include cerebellar dysfunction, cancer, diabetes, and premature aging. We genetically separated the activation of ATM by DNA damage from that by oxidative stress using separation-of-function mutations. We found that deficient activation of ATM by the Mre11-Rad50-Nbs1 complex and DNA double-strand breaks resulted in loss of cell viability, checkpoint activation, and DNA end resection in response to DNA damage. In contrast, loss of oxidative activation of ATM had minimal effects on DNA damage-related outcomes but blocked ATM-mediated initiation of checkpoint responses after oxidative stress and resulted in deficiencies in mitochondrial function and autophagy. In addition, expression of a variant ATM incapable of activation by oxidative stress resulted in widespread protein aggregation. These results indicate a direct relationship between the mechanism of ATM activation and its effects on cellular metabolism and DNA damage responses in human cells and implicate ATM in the control of protein homeostasis. Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

High-Speed Data Recorder for Space, Geodesy, and Other High-Speed Recording Applications

Science.gov (United States)

Taveniku, Mikael

2013-01-01

A high-speed data recorder and replay equipment has been developed for reliable high-data-rate recording to disk media. It solves problems with slow or faulty disks, multiple disk insertions, high-altitude operation, reliable performance using COTS hardware, and long-term maintenance and upgrade path challenges. The current generation data recor - ders used within the VLBI community are aging, special-purpose machines that are both slow (do not meet today's requirements) and are very expensive to maintain and operate. Furthermore, they are not easily upgraded to take advantage of commercial technology development, and are not scalable to multiple 10s of Gbit/s data rates required by new applications. The innovation provides a softwaredefined, high-speed data recorder that is scalable with technology advances in the commercial space. It maximally utilizes current technologies without being locked to a particular hardware platform. The innovation also provides a cost-effective way of streaming large amounts of data from sensors to disk, enabling many applications to store raw sensor data and perform post and signal processing offline. This recording system will be applicable to many applications needing realworld, high-speed data collection, including electronic warfare, softwaredefined radar, signal history storage of multispectral sensors, development of autonomous vehicles, and more.

Scenarios for control and data flows in multiprotocol over ATM

Science.gov (United States)

Kujoory, Ali

1997-10-01

The multiprotocol over ATM (MPOA), specified by the ATM Forum, provides an architecture for transfer of Internetwork layer packets (Layer 3 datagram such as IP, IPX) over ATM subnets or across the emulated LANs. MPOA provides shortcuts that bypass routers to avoid router bottlenecks. It is a grand union of some of the existing standards such as LANE by the ATM Forum, NHRP by the IETF, and the Q.2931 by ITU. The intent of this paper is to clarify the data flows between pairs of source and destination hosts in an MPOA system. It includes scenarios for both the intra- and inter-subnet flows between different pairs of MPOA end-systems. The intrasubnet flows simply use LANE for address resolution or data transfer. The inter-subnet flows may use a default path for short-lived flows or a shortcut for long-lived flows. The default path uses the LANE and router capabilities. The shortcut path uses LANE plus NHRP for ATM address resoluton. An ATM virtual circuit is established before the data transfer. This allows efficient transfer of internetwork layer packets over ATM for real-time applications.

Molecular and functional characterization of a human ATM gene analogue at Arabidopsis thaliana; Caracterisation moleculaire et Fonctionnelle d'un Homologue du gene humain ATM chez Arabidopsis thaliana

Energy Technology Data Exchange (ETDEWEB)

Garcia, V.

2001-12-15

The human ATM gene, whose inactivation is responsible for the human disease ataxia telangiectasia is conserved throughout the Eukaryotes and plays an important role in the cellular responses to DNA damage, in particular to DNA double-strand breaks (DSBs). Here we describe the identification of an Arabidopsis thaliana homologue of ATM (AtATM), and the molecular and cytological characterization of plants, hereafter called atm, carrying a disrupting T-DNA insertion in this gene. AtATM covers a 32 kb region on chromosome 3. The AtATM transcript has a complex structure, is 12 kb long and formed by 79 exons. The transcriptional level of AtATM is very low in all the tissues tested, and does not vary after exposure to ionizing radiations (IR). In atm plants, the protein is not detected suggesting the mutants are null. The atm mutants are partially sterile. Aberrant segregation of chromosomes during meiosis I on both male and female sides account for this sterility. However, meiotic recombination frequency is normal. Mutant plants are also hypersensitive to gamma rays and methyl methane sulfonate, but not to UV-B, pointing to a specific defect of atm mutants in the response to DNA DSBs. In plants, ionizing radiations induce a strong, rapid and transient transcriptional activation of genes involved in the cellular response to or the repair of DSBs. This transcriptional regulation of AtRAD51, AtPARP1, atGR1 and AtL1G4 is lost in the atm mutants . The absence of AtRAD51 induction associated with ionizing radiation sensitivity suggest that AtAtm play an important function in DSB repair by homologous recombination. In addition we show that homologous intra-chromosomal recombination frequency is elevated in the mutant comparing to wild-type, with or without gamma irradiation. These results show the implication of AtAtm in the genomic stability. (author)

Parametric studies on floating pad journal bearing for high speed cryogenic turboexpanders

Science.gov (United States)

Jain, A.; Jadhav, M. M.; Karimulla, S.; Chakravarty, A.

2017-12-01

Most modern medium and large capacity helium liquefaction/refrigeration plants employ high speed cryogenic turboexpanders in their refrigeration/liquefaction cycles as active cooling devices. The operating speed of these turboexpanders is in the range of 3000-5000 Hz and hence specialized types of bearings are required. Floating pad journal bearing, which is a special type of tilting pad journal bearing, where mechanical pivots are absent and pads are fully suspended in gas, can be a good solution for stable operation of these high speed compact rotors. The pads are separated from shaft as well as from housing by fluid film between them, and both these sides of pad are interconnected by a network of feed holes. The work presented in this article aims to characterize floating pad journal bearings through parametric studies. The steady state performance characteristics of the bearing are represented by load capacity, stiffness coefficients and heat generation rate of the bearing. The geometrical parameters such as bearing clearances, preload of pads, etc. are varied and performance characteristics of the floating pad journal bearing are studied and presented. The dependence of stiffness coefficients on rotational speed of shaft is also analyzed.

ATM

Directory of Open Access Journals (Sweden)

Boo Yong Ahn

1999-01-01

Full Text Available We model the error control of the partial buffer sharing of ATM by a queueing system M1,M2/G/1/K+1 with threshold and instantaneous Bernoulli feedback. We first derive the system equations and develop a recursive method to compute the loss probabilities at an arbitrary time epoch. We then build an approximation scheme to compute the mean waiting time of each class of cells. An algorithm is developed for finding the optimal threshold and queue capacity for a given quality of service.

The high speed civil transport and NASA's High Speed Research (HSR) program

Science.gov (United States)

Shaw, Robert J.

1994-01-01

Ongoing studies being conducted not only in this country but in Europe and Asia suggest that a second generation supersonic transport, or High-Speed Civil Transport (HSCT), could become an important part of the 21st century international air transportation system. However, major environmental compatibility and economic viability issues must be resolved if the HSCT is to become a reality. This talk will overview the NASA High-Speed Research (HSR) program which is aimed at providing the U.S. industry with a technology base to allow them to consider launching an HSCT program early in the next century. The talk will also discuss some of the comparable activities going on within Europe and Japan.

Models for setting ATM parameter values

DEFF Research Database (Denmark)

Blaabjerg, Søren; Gravey, A.; Romæuf, L.

1996-01-01

essential to set traffic characteristic values that are relevant to the considered cell stream, and that ensure that the amount of non-conforming traffic is small. Using a queueing model representation for the GCRA formalism, several methods are available for choosing the traffic characteristics. This paper......In ATM networks, a user should negotiate at connection set-up a traffic contract which includes traffic characteristics and requested QoS. The traffic characteristics currently considered are the Peak Cell Rate, the Sustainable Cell Rate, the Intrinsic Burst Tolerance and the Cell Delay Variation...... (CDV) tolerance(s). The values taken by these traffic parameters characterize the so-called ''Worst Case Traffic'' that is used by CAC procedures for accepting a new connection and allocating resources to it. Conformance to the negotiated traffic characteristics is defined, at the ingress User...

ATM Coastal Topography-Texas, 2001: UTM Zone 15

Science.gov (United States)

Klipp, Emily S.; Nayegandhi, Amar; Brock, John C.; Sallenger, A.H.; Bonisteel, Jamie M.; Yates, Xan; Wright, C. Wayne

2009-01-01

These remotely sensed, geographically referenced elevation measurements of lidar-derived first-surface (FS) topography were produced collaboratively by the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL, and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of a portion of the Texas coastline within UTM zone 15, from Matagorda Peninsula to Galveston Island, acquired October 12-13, 2001. The datasets are made available for use as a management tool to research scientists and natural-resource managers. An innovative scanning lidar instrument originally developed by NASA, and known as the Airborne Topographic Mapper (ATM), was used during data acquisition. The ATM system is a scanning lidar system that measures high-resolution topography of the land surface and incorporates a green-wavelength laser operating at pulse rates of 2 to 10 kilohertz. Measurements from the laser-ranging device are coupled with data acquired from inertial navigation system (INS) attitude sensors and differentially corrected global positioning system (GPS) receivers to measure topography of the surface at accuracies of +/-15 centimeters. The nominal ATM platform is a Twin Otter or P-3 Orion aircraft, but the instrument may be deployed on a range of light aircraft. Elevation measurements were collected over the survey area using the ATM system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of lidar data in an interactive or batch mode. Modules for presurvey flight-line definition, flight-path plotting, lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant

Characterization of Early and Late Adopters of ATM Card in Indian Banking Industry

Directory of Open Access Journals (Sweden)

Kamalpreet Kaur

2012-10-01

Full Text Available The present study deals with affect of adoption pattern of the ATMs by banks on its characteristics. With the exploration of various characteristics of the banks like Size, Profi tability, Efficiency, Cost of Operations, Asset quality and Credit risk, Financing Pattern, Diversifi cation and Age etc.; the study has tried to differentiate between the early and late adopter category of the banks regarding ATM cards. The banks have been categorized into early and late adopters on the basis of their adoption period. For this purpose, 50 scheduled commercial banks consisting of 27 Public Sector Banks and 23 Private Sector Banks have been taken as sample to investigate the various aspects of and early adopter banks in comparison to late adopter banks. The time period of the study is 20 years i.e. from 1991 to 2010. It can be concluded that the initiators and fi rst movers take advantage over the late adopters and laggards. They have found to perform better in terms of various parameters. Overall, the early adopter banks are larger in size, more diversifi ed, having lesser branches, more market share and wide ATM network as compared to late adopter ones. Thus, the empirical results evidently reveal that the both the groups have their own different characteristics.

Using a High-Speed Camera to Measure the Speed of Sound

Science.gov (United States)

Hack, William Nathan; Baird, William H.

2012-01-01

The speed of sound is a physical property that can be measured easily in the lab. However, finding an inexpensive and intuitive way for students to determine this speed has been more involved. The introduction of affordable consumer-grade high-speed cameras (such as the Exilim EX-FC100) makes conceptually simple experiments feasible. Since the…

3rd ENRI International Workshop on ATM/CNS

CERN Document Server

2014-01-01

The Electronic Navigation Research Institute (ENRI) held its third　International Workshop on ATM / CNS in 2013 with the theme of "Drafting　the future sky". There is worldwide activity taking place in the research and development　of modern air traffic management (ATM) and its enabling technologies in　Communication, Navigation and Surveillance (CNS). Pioneering work is necessary to contribute to the global harmonization　of air traffic management and control. At this workshop, leading experts　in  research, industry and academia from around the world met to share　their ideas and approaches on ATM/CNS related topics.

ATM-Deficient Colorectal Cancer Cells Are Sensitive to the PARP Inhibitor Olaparib.

Science.gov (United States)

Wang, Chen; Jette, Nicholas; Moussienko, Daniel; Bebb, D Gwyn; Lees-Miller, Susan P

2017-04-01

The ataxia telangiectasia mutated (ATM) protein kinase plays a central role in the cellular response to DNA damage. Loss or inactivation of both copies of the ATM gene (ATM) leads to ataxia telangiectasia, a devastating childhood condition characterized by neurodegeneration, immune deficiencies, and cancer predisposition. ATM is also absent in approximately 40% of mantle cell lymphomas (MCLs), and we previously showed that MCL cell lines with loss of ATM are sensitive to poly-ADP ribose polymerase (PARP) inhibitors. Next-generation sequencing of patient tumors has revealed that ATM is altered in many human cancers including colorectal, lung, prostate, and breast. Here, we show that the colorectal cancer cell line SK-CO-1 lacks detectable ATM protein expression and is sensitive to the PARP inhibitor olaparib. Similarly, HCT116 colorectal cancer cells with shRNA depletion of ATM are sensitive to olaparib, and depletion of p53 enhances this sensitivity. Moreover, HCT116 cells are sensitive to olaparib in combination with the ATM inhibitor KU55933, and sensitivity is enhanced by deletion of p53. Together our studies suggest that PARP inhibitors may have potential for treating colorectal cancer with ATM dysfunction and/or colorectal cancer with mutation of p53 when combined with an ATM kinase inhibitor. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

ATM Technology and Banking System in West African Sub-Region ...

African Journals Online (AJOL)

Automated Teller Machine (ATM) technology has had its significant impact in banking system in Nigeria and some other West African Countries. The most significant impact of ATM technology is the customer's ability to withdraw money outside banking hours. But this feat achieved by ATM technology is not without ...

Experimental Characterization of LTE Wireless Links in High-Speed Trains

Directory of Open Access Journals (Sweden)

Tomás Domínguez-Bolaño

2017-01-01

Full Text Available Multimedia and data-based services experienced a nonstopping growth over the last few years. People are continuously on the move using devices to access multimedia contents or other data-based services. Due to this, railway companies are showing a great interest in deploying broadband mobile wireless networks in high-speed-trains with the aim of supporting both passenger services provisioning as well as automatic train control and signaling. Nowadays, the most widely used technology for communications between trains and the railway infrastructure is GSM for Railways (GSM-R; however, it has limited capabilities to support such advanced services. Due to its success in the mass market, Long Term Evolution (LTE seems to be the best candidate to substitute GSM-R. In this paper, we experimentally characterize the downlink between an LTE Evolved NodeB (eNodeB and a high-speed train in a commercial high-speed line. We consider two links: the one between the eNodeB and the antennas placed outdoors on the train roof, and the direct link between the eNodeB and a receiver inside the train. Such a characterization consists in assessing the path loss, the Signal to Noise Ratio, the K-Factor, the Power Delay Profile, the delay spread, and the Doppler Power Spectral Density.

33 CFR 84.24 - High-speed craft.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false High-speed craft. 84.24 Section... RULES ANNEX I: POSITIONING AND TECHNICAL DETAILS OF LIGHTS AND SHAPES § 84.24 High-speed craft. (a) The masthead light of high-speed craft with a length to breadth ratio of less than 3.0 may be placed at a...

Modern trends in designing high-speed trains

Directory of Open Access Journals (Sweden)

Golubović Snežana D.

2015-01-01

Full Text Available Increased advantages of railway transportation systems over other types of transportation systems in the past sixty years have been a result of an intensive development of the new generations of high-speed trains. Not only do these types of trains comply with the need for increased speed of transportation and make the duration of the journey shorter, but they also meet the demands for increased reliability, safety and direct application of energy efficiency to the transportation system itself. Along with increased train speed, the motion resistance is increased as well, whereby at speeds over 200 km/h the proportion of air resistance becomes the most dominant member. One of the most efficient measures for reducing air resistance, as well as other negative consequences of high-speed motion, is the development of the aerodynamic shape of the train. This paper presents some construction solutions that affect the aerodynamic properties of high-speed trains, first and foremost, the nose shape, as well as the similarities and differences of individual subsystems necessary for the functioning of modern high-speed rail systems. We analysed two approaches to solving the problem of the aerodynamic shape of the train and the appropriate infrastructure using the examples of Japan and France. Two models of high-speed trains, Shinkansen (Japan and TGV, i.e. AGV (France, have been discussed.

ATM, radiation, and the risk of second primary breast cancer.

Science.gov (United States)

Bernstein, Jonine L; Concannon, Patrick

2017-10-01

It was first suggested more than 40 years ago that heterozygous carriers for the human autosomal recessive disorder Ataxia-Telangiectasia (A-T) might also be at increased risk for cancer. Subsequent studies have identified the responsible gene, Ataxia-Telangiectasia Mutated (ATM), characterized genetic variation at this locus in A-T and a variety of different cancers, and described the functions of the ATM protein with regard to cellular DNA damage responses. However, an overall model of how ATM contributes to cancer risk, and in particular, the role of DNA damage in this process, remains lacking. This review considers these questions in the context of contralateral breast cancer (CBC). Heterozygous carriers of loss of function mutations in ATM that are A-T causing, are at increased risk of breast cancer. However, examination of a range of genetic variants, both rare and common, across multiple cancers, suggests that ATM may have additional effects on cancer risk that are allele-dependent. In the case of CBC, selected common alleles at ATM are associated with a reduced incidence of CBC, while other rare and predicted deleterious variants may act jointly with radiation exposure to increase risk. Further studies that characterize germline and somatic ATM mutations in breast cancer and relate the detected genetic changes to functional outcomes, particularly with regard to radiation responses, are needed to gain a complete picture of the complex relationship between ATM, radiation and breast cancer.

Regulation of the DNA Damage Response by DNA-PKcs Inhibitory Phosphorylation of ATM.

Science.gov (United States)

Zhou, Yi; Lee, Ji-Hoon; Jiang, Wenxia; Crowe, Jennie L; Zha, Shan; Paull, Tanya T

2017-01-05

Ataxia-telangiectasia mutated (ATM) regulates the DNA damage response as well as DNA double-strand break repair through homologous recombination. Here we show that ATM is hyperactive when the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is chemically inhibited or when the DNA-PKcs gene is deleted in human cells. Pre-incubation of ATM protein with active DNA-PKcs also significantly reduces ATM activity in vitro. We characterize several phosphorylation sites in ATM that are targets of DNA-PKcs and show that phospho-mimetic mutations at these residues significantly inhibit ATM activity and impair ATM signaling upon DNA damage. In contrast, phospho-blocking mutations at one cluster of sites increase the frequency of apoptosis during normal cell growth. DNA-PKcs, which is integral to the non-homologous end joining pathway, thus negatively regulates ATM activity through phosphorylation of ATM. These observations illuminate an important regulatory mechanism for ATM that also controls DNA repair pathway choice. Copyright © 2017 Elsevier Inc. All rights reserved.

Health ATMs in Saudi Arabia: A Perspective.

Science.gov (United States)

Aldosari, Bakheet

2017-06-01

Health ATMs are terminals which are connected to a centrally located database storing patients' electronic healthcare records (EHR). These machines are capable of collecting information in a far superior fashion than humans and are also able to rectify obsolete data in a manner that humans are generally not inclined to. The main goal of this study is to assess the importance of adopting health ATMs in the Kingdom of Saudi Arabia (KSA), which can improve the confidence of patients, reward health self-management, and achieve positive health outcomes through their easy-to-use applications that are secure and accessible through various devices. Strength, Weakness, Opportunity, and Threat (SWOT) analysis was used to assess the efficiency of adopting health ATMs in KSA and reveal the said characteristics. Three focus groups assembled in the cities of Riyadh, Jeddah and Dammam during the period 2013-2014. The groups consisted of individuals experienced in the function of health ATMs. It was found that the sector possessed a number of strengths that would help it in reaching the goals outlined therein, thereby achieving successful outcomes. Health ATMs could be a promising new advancement in the field of health if the project were to be planned and implemented correctly. Their benefits would consequently reach organizational and national levels. It is, therefore, crucial to educate the project managers about the benefits of learning from others as well as educating them about the needs and the requirements of the concerned organization.

Convective transport in ATM simulations and its relation to the atmospheric stability conditions

Science.gov (United States)

Kusmierczyk-Michulec, Jolanta

2017-04-01

The International Monitoring System (IMS) developed by the Comprehensive Nuclear-Test-Ban Treaty Organization (CTBTO) is a global system of monitoring stations, using four complementary technologies: seismic, hydroacoustic, infrasound and radionuclide. Data from all stations, belonging to IMS, are collected and transmitted to the International Data Centre (IDC) in Vienna, Austria. The radionuclide network comprises 80 stations, of which more than 60 are certified. The aim of radionuclide stations is a global monitoring of radioactive aerosols and radioactive noble gases, in particular xenon isotopes, supported by the atmospheric transport modeling (ATM). One of the important noble gases, monitored on a daily basis, is radioxenon. It can be produced either during a nuclear explosion with a high fission yield, and thus be considered as an important tracer to prove the nuclear character of an explosion, or be emitted from nuclear power plants (NPPs) or from isotope production facilities (IPFs). To investigate the transport of xenon emissions, the Provisional Technical Secretariat (PTS) operates an Atmospheric Transport Modelling (ATM) system based on the Lagrangian Particle Dispersion Model FLEXPART. To address the question whether including the convective transport in ATM simulations will change the results significantly, the differences between the outputs with the convective transport turned off and turned on, were computed and further investigated taking into account the atmospheric stability conditions. For that purpose series of 14 days forward simulations, with convective transport and without it, released daily in the period January 2011 to February 2012, were analysed. The release point was at the ANSTO facility in Australia. The unique opportunity of having access to both daily emission values for ANSTO as well as measured Xe-133 activity concentration (AC) values at the IMS stations, gave a chance to validate the simulations.

Fabrication and characterization of MCC [Materials Characterization Center] approved testing material: ATM-10 glass

International Nuclear Information System (INIS)

Maupin, G.D.; Bowen, W.M.; Daniel, J.L.

1988-04-01

The Materials Characterization Center ATM-10 glass represents a reference commercial high-level waste form similar to that which will be produced by the West Valley Nuclear Service Co. Inc., West Valley, New York. The target composition and acceptable range of composition were defined by the sponsor, West Valley Nuclear Service. The ATM-10 glass was produced in accordance with the Pacific Northwest Laboratory QA Manual for License-Related Programs, MCC technical procedures, and MCC QA Plan that were in effect during the course of the work. The method and procedure to be used in the fabrication and characterization of the ATM-10 glass were specified in two run plans for glass preparation and a characterization plan. All of the ATM-10 glass was produced in the form of bars 1.9 /times/ 1.9 /times/ 10 cm nominal size, and 93 g nominal mass. A total of 15 bars of ATM-10 glass weighing 1394 g was produced. The production bars were characterized to determine the mean composition, oxidation state, and microstructure of the ATM-10 product. Table A summarizes the characterization results. The ATM-10 glass meets all specifications. The elemental composition and oxidation state of the glass are within the specifications of the client. Visually, the ATM-10 glass bars appear uniformly glassy and generally without exterior features. Microscopic examination revealed low (less than 2 wt %) concentractions of 3-μm iron-chrome (suspected spinel) crystals and /approximately/0.5-μm ruthenium inclusions scattered randomly throughout the glassy matrix. Closed porosity, with pores ranging in diameter from 5 to 250 μm, was observed in all samples. 4 refs., 10 figs., 21 tabs

Millimeter Wave Hybrid Photonic Wireless Links for High-Speed Wireless Access and Mobile Fronthaul

DEFF Research Database (Denmark)

Rommel, Simon

As the introduction of the fifth generation of mobile services (5G) is set to revolutionize the way people, devices and machines connect, the changes to the underlying networks and technologies are no less drastic. The massive increase in user and data capacity, as well as the decrease in latency...... networks. In summary, the work presented in this thesis has regarded a multitude of aspects of millimeter wave hybrid photonic wireless links, expanding upon the state of the art and showing their feasibility for use in fifth generation mobile and high speed wireless access networks – hopefully bringing...

Preventive Methods for ATM Mode Control

OpenAIRE

Ivan Baronak; Robert Trska

2004-01-01

Broadband transfer mode ATM represent one of alternative solutions for growing requirements on transfer capabilities. Its advantage is an effort for provisions of guaranteed quality of transport services with preservations of high transfer rate. This property is covered by several mechanisms, which role is to control not only the traffic of existing connections, but also the admission of new ones and prevent the violation of requirements on transport quality of existing and new connections.

Changes of indicators of high-speed and high-speed and power preparedness at volleyball players of 12–13 years old

Directory of Open Access Journals (Sweden)

Oleg Shevchenko

2016-04-01

Full Text Available Purpose: to define changes of indicators of high-speed and high-speed and power preparedness of volleyball players of 12–13 years old. Material & Methods: the test exercises, which are recommended by the training program of CYSS on volleyball, were used for the definition of the level of development of high-speed and high-speed and power abilities of volleyball players. 25 young volleyball players from the group of the previous basic preparation took part in the experiment. Sports experience of sportsmen is 3–4 years. The analysis of scientifically-methodical literature, pedagogical testing, pedagogical experiment, methods of mathematical statistics were carried out. Results: the analyzed level of high-speed and high-speed and power abilities of volleyball players. Conclusions: the results had reliable changes (t=2,2–2,4 at р<0,05 of the level of high-speed and high-speed and power abilities of volleyball players of 12–13years old in the experimental group at the end of the experiment, except run on 30 m that demonstrates a positive influence of application of special exercises in the educational-training process.

Performance Optimization Design for a High-Speed Weak FBG Interrogation System Based on DFB Laser

Directory of Open Access Journals (Sweden)

Yiqiang Yao

2017-06-01

Full Text Available A performance optimization design for a high-speed fiber Bragg grating (FBG interrogation system based on a high-speed distributed feedback (DFB swept laser is proposed. A time-division-multiplexing sensor network with identical weak FBGs is constituted to realize high-capacity sensing. In order to further improve the multiplexing capacity, a waveform repairing algorithm is designed to extend the dynamic demodulation range of FBG sensors. It is based on the fact that the spectrum of an FBG keeps stable over a long period of time. Compared with the pre-collected spectra, the distorted spectra waveform are identified and repaired. Experimental results show that all the identical weak FBGs are distinguished and demodulated at the speed of 100 kHz with a linearity of above 0.99, and the range of dynamic demodulation is extended by 40%.

Performance Optimization Design for a High-Speed Weak FBG Interrogation System Based on DFB Laser.

Science.gov (United States)

Yao, Yiqiang; Li, Zhengying; Wang, Yiming; Liu, Siqi; Dai, Yutang; Gong, Jianmin; Wang, Lixin

2017-06-22

A performance optimization design for a high-speed fiber Bragg grating (FBG) interrogation system based on a high-speed distributed feedback (DFB) swept laser is proposed. A time-division-multiplexing sensor network with identical weak FBGs is constituted to realize high-capacity sensing. In order to further improve the multiplexing capacity, a waveform repairing algorithm is designed to extend the dynamic demodulation range of FBG sensors. It is based on the fact that the spectrum of an FBG keeps stable over a long period of time. Compared with the pre-collected spectra, the distorted spectra waveform are identified and repaired. Experimental results show that all the identical weak FBGs are distinguished and demodulated at the speed of 100 kHz with a linearity of above 0.99, and the range of dynamic demodulation is extended by 40%.

Reducing Heating In High-Speed Cinematography

Science.gov (United States)

Slater, Howard A.

1989-01-01

Infrared-absorbing and infrared-reflecting glass filters simple and effective means for reducing rise in temperature during high-speed motion-picture photography. "Hot-mirror" and "cold-mirror" configurations, employed in projection of images, helps prevent excessive heating of scenes by powerful lamps used in high-speed photography.

Modeling of High-Speed InP DHBTs using Electromagnetic Simulation Based De-embedding

DEFF Research Database (Denmark)

Johansen, Tom Keinicke; Krozer, Viktor; Konczykowska, Agnieszka

2006-01-01

In this paper an approach for high-speed InP DHBT modeling valid to 110 GHz is reported. Electromagnetic (EM) simulation is applied to predict the embedded network model caused by pad parasitics. The form of the parasitic network calls for a 4-step de-embedding approach. Applying direct parameter...... extraction on the de-embedded device response leads to accurate small-signal model description of the InP DHBT. An parameter extraction approach is described for the Agilent HBT model, which assures consistency between large-signal and bias-dependent smallsignal modeling....

High speed data acquisition

International Nuclear Information System (INIS)

Cooper, P.S.

1997-07-01

A general introduction to high speed data acquisition system techniques in modern particle physics experiments is given. Examples are drawn from the SELEX(E78 1) high statistics charmed baryon production and decay experiment now taking data at Fermilab

High speed in Portugal - metamorphosis from planning construction measures to a national railway concept; Hochgeschwindigkeit in Portugal - von der Bauplanung zu einem nationalen Eisenbahnkonzept

Energy Technology Data Exchange (ETDEWEB)

Stohler, Werner [Beratungfirma SMA und Partner AG, Zuerich (Switzerland)

2010-03-15

Portugal is planning the construction of two high-speed railway lines (using the UIC gauge of 1435 mm) between Lisbon and Madrid and between Lisbon and Porto. Integration with the country's existing Iberian-gauge network will be achieved in some places by laying three-rail tracks and in other places by running trains with adjustable wheel gauges. The adaptations to the legacy network triggered by the plants for the high-speed lines include, inter alia, a rearrangement of the networks of the regional-express services in and around Lisbon and Porto. (orig.)

Optimizing Low Speed VoIP Network for Rural Next Generation Network (R-NGN

Directory of Open Access Journals (Sweden)

Yoanes Bandung

2007-11-01

Full Text Available In this research, we propose an optimization method based-on E-Model for designing an efficient low speed VoIP network for Rural Next Generation Network (R-NGN. We are choosing 128 kbps and 256 kbps bandwidth as the typical community link to be used in the designing of R-NGN infrastructure. The method is based on selection of some VoIP network parameters such as voice coder, communication protocol, packet loss level, network utilization and resource allocation. We draw analytic approach for achieving rating value (R of E-model that represent level of quality of service. In this approach, we focus on delay and packet loss calculation to find the rating value. We state the rating value = 70 as minimum level of quality of service for each call, equivalent to 3.6 of Mean Opinion Score (MOS. In our experiments, either G.723.1 5.3 kbps or G.729 is chosen for maximizing the number of VoIP calls, it depends on link utilization and level of packet loss.

Wake flow characteristics at high wind speed

DEFF Research Database (Denmark)

Aagaard Madsen, Helge; Larsen, Torben J.; Larsen, Gunner Chr.

2016-01-01

Wake flow characteristic at high wind speeds is the main subject of this paper. Although the wake losses decrease at high wind speeds it has been found in a recent study that for multiple wake inflow the increase in loading due to wake effects are substantial even at wind speeds well above rated ...

KONTROLÖR ALAN AĞI ESASLI BİR ATM ALAN TAŞITININ TASARLANMASI

Directory of Open Access Journals (Sweden)

Mahmut TENRUH

2006-03-01

Full Text Available Kontrolör Alan Ağı (KAA taşıtı başlangıçta otomotiv uygulamaları için önerilmiş fakat düşük maliyeti yüksek hızı ve güvenilirliği sayesinde endüstriyel dağılımlı gerçek zamanlı kontrol uygulamalarında da bir standart haline gelmiştir. ATM veri, ses ve görüntü gibi tüm haberleşme türlerini bir network yapısı içerisinde birleştirmeyi hedefleyen hızlı bir ağ teknolojisidir. Ethernet ve Token Ring gibi mevcut ağ türlerinin ATM ile bağlanması için çeşitli çalışmalar sürdürülmüştür. Kontrol Taşıtı haberleşmesinin de bu çerçevede ele alınması önem taşımaktadır. Bu çalışma ATM teknolojisinin Kontrol Taşıtı haberleşmesi ile birlikte kullanılmasını amaçlamaktadır. Bu kapsamda KAA esaslı ATM Taşıt yapısı sunulmaktadır. Bu yapı aynı zamanda Kontrol Taşıt ağlarının ATM ağları ile doğrudan bağlanabilmesi için de bir imkan sunmaktadır. Önerilen modelin geçerliliğini görmek amacıyla simülasyon çalışmaları yürütülmüş ve sonuçlar sistemin ek avantajlarla uygulanabilir olduğunu göstermiştir.

Neurodegeneration in ataxia-telangiectasia: Multiple roles of ATM kinase in cellular homeostasis.

Science.gov (United States)

Choy, Kay Rui; Watters, Dianne J

2018-01-01

Ataxia-telangiectasia (A-T) is characterized by neuronal degeneration, cancer, diabetes, immune deficiency, and increased sensitivity to ionizing radiation. A-T is attributed to the deficiency of the protein kinase coded by the ATM (ataxia-telangiectasia mutated) gene. ATM is a sensor of DNA double-strand breaks (DSBs) and signals to cell cycle checkpoints and the DNA repair machinery. ATM phosphorylates numerous substrates and activates many cell-signaling pathways. There has been considerable debate about whether a defective DNA damage response is causative of the neurological aspects of the disease. In proliferating cells, ATM is localized mainly in the nucleus; however, in postmitotic cells such as neurons, ATM is mostly cytoplasmic. Recent studies reveal an increasing number of roles for ATM in the cytoplasm, including activation by oxidative stress. ATM associates with organelles including mitochondria and peroxisomes, both sources of reactive oxygen species (ROS), which have been implicated in neurodegenerative diseases and aging. ATM is also associated with synaptic vesicles and has a role in regulating cellular homeostasis and autophagy. The cytoplasmic roles of ATM provide a new perspective on the neurodegenerative process in A-T. This review will examine the expanding roles of ATM in cellular homeostasis and relate these functions to the complex A-T phenotype. Developmental Dynamics 247:33-46, 2018. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Receiver-Assisted Congestion Control to Achieve High Throughput in Lossy Wireless Networks

Science.gov (United States)

Shi, Kai; Shu, Yantai; Yang, Oliver; Luo, Jiarong

2010-04-01

Many applications would require fast data transfer in high-speed wireless networks nowadays. However, due to its conservative congestion control algorithm, Transmission Control Protocol (TCP) cannot effectively utilize the network capacity in lossy wireless networks. In this paper, we propose a receiver-assisted congestion control mechanism (RACC) in which the sender performs loss-based control, while the receiver is performing delay-based control. The receiver measures the network bandwidth based on the packet interarrival interval and uses it to compute a congestion window size deemed appropriate for the sender. After receiving the advertised value feedback from the receiver, the sender then uses the additive increase and multiplicative decrease (AIMD) mechanism to compute the correct congestion window size to be used. By integrating the loss-based and the delay-based congestion controls, our mechanism can mitigate the effect of wireless losses, alleviate the timeout effect, and therefore make better use of network bandwidth. Simulation and experiment results in various scenarios show that our mechanism can outperform conventional TCP in high-speed and lossy wireless environments.

14 CFR 25.253 - High-speed characteristics.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false High-speed characteristics. 25.253 Section...-speed characteristics. (a) Speed increase and recovery characteristics. The following speed increase and recovery characteristics must be met: (1) Operating conditions and characteristics likely to cause...

Gender, academic achievement, and ownership of ATM as predictors of accounting students’ financial literacy

Science.gov (United States)

Susanti; Hardini, H. T.

2018-01-01

This study examined the relationships between GPA, gender, and ownership of ATM on accounting students’ financial literacy (n = 184). Financial literacy was assessed using a paper-and-pencil objective (multiple choice) test measuring general knowledge of finance, income, money management savings, loans, and investment. Gender and GPA data were obtained from the university records. Regression analysis found that GPA and ownership of ATM were associated with financial literacy, but gender was not. Female students with an ownership of ATM and those with a high GPA were found to be superior to males. The implication of this research is that students are expected to increase their GPA and utilize financial facilities in the form of ownership ATM and other financial instruments so as to increase financial literacy. In addition, the need for financial literacy training from related parties to improve financial literacy for students who have low financial literacy.

Chicago-St. Louis high speed rail plan

International Nuclear Information System (INIS)

Stead, M.E.

1994-01-01

The Illinois Department of Transportation (IDOT), in cooperation with Amtrak, undertook the Chicago-St. Louis High Speed Rail Financial and Implementation Plan study in order to develop a realistic and achievable blueprint for implementation of high speed rail in the Chicago-St. Louis corridor. This report presents a summary of the Price Waterhouse Project Team's analysis and the Financial and Implementation Plan for implementing high speed rail service in the Chicago-St. Louis corridor

Chicago-St. Louis high speed rail plan

Energy Technology Data Exchange (ETDEWEB)

Stead, M.E.

1994-12-31

The Illinois Department of Transportation (IDOT), in cooperation with Amtrak, undertook the Chicago-St. Louis High Speed Rail Financial and Implementation Plan study in order to develop a realistic and achievable blueprint for implementation of high speed rail in the Chicago-St. Louis corridor. This report presents a summary of the Price Waterhouse Project Team`s analysis and the Financial and Implementation Plan for implementing high speed rail service in the Chicago-St. Louis corridor.

Ultra-high-speed Optical Signal Processing using Silicon Photonics

DEFF Research Database (Denmark)

Oxenløwe, Leif Katsuo; Ji, Hua; Jensen, Asger Sellerup

with a photonic layer on top to interconnect them. For such systems, silicon is an attractive candidate enabling both electronic and photonic control. For some network scenarios, it may be beneficial to use optical on-chip packet switching, and for high data-density environments one may take advantage...... of the ultra-fast nonlinear response of silicon photonic waveguides. These chips offer ultra-broadband wavelength operation, ultra-high timing resolution and ultra-fast response, and when used appropriately offer energy-efficient switching. In this presentation we review some all-optical functionalities based...... on silicon photonics. In particular we use nano-engineered silicon waveguides (nanowires) [1] enabling efficient phasematched four-wave mixing (FWM), cross-phase modulation (XPM) or self-phase modulation (SPM) for ultra-high-speed optical signal processing of ultra-high bit rate serial data signals. We show...

Lubrication and cooling for high speed gears

Science.gov (United States)

Townsend, D. P.

1985-01-01

The problems and failures occurring with the operation of high speed gears are discussed. The gearing losses associated with high speed gearing such as tooth mesh friction, bearing friction, churning, and windage are discussed with various ways shown to help reduce these losses and thereby improve efficiency. Several different methods of oil jet lubrication for high speed gearing are given such as into mesh, out of mesh, and radial jet lubrication. The experiments and analytical results for the various methods of oil jet lubrication are shown with the strengths and weaknesses of each method discussed. The analytical and experimental results of gear lubrication and cooling at various test conditions are presented. These results show the very definite need of improved methods of gear cooling at high speed and high load conditions.

Towards realising high-speed large-bandwidth quantum memory

Institute of Scientific and Technical Information of China (English)

SHI BaoSen; DING DongSheng

2016-01-01

Indispensable for quantum communication and quantum computation,quantum memory executes on demand storage and retrieval of quantum states such as those of a single photon,an entangled pair or squeezed states.Among the various forms of quantum memory,Raman quantum memory has advantages forits broadband and high-speed characteristics,which results in a huge potential for applications in quantum networks and quantum computation.However,realising Raman quantum memory with true single photons and photonic entanglementis challenging.In this review,after briefly introducing the main benchmarks in the development of quantum memory and describing the state of the art,we focus on our recent experimental progress inquantum memorystorage of quantum states using the Raman scheme.

ATM-mediated Snail Serine 100 phosphorylation regulates cellular radiosensitivity

International Nuclear Information System (INIS)

Boohaker, Rebecca J.; Cui, Xiaoli; Stackhouse, Murray; Xu, Bo

2013-01-01

Purpose: Activation of the DNA damage responsive protein kinase ATM is a critical step for cellular survival in response to ionizing irradiation (IR). Direct targets of ATM regulating radiosensitivity remain to be fully investigated. We have recently reported that ATM phosphorylates the transcriptional repressor Snail on Serine 100. We aimed to further study the functional significance of ATM-mediated Snail phosphorylation in response to IR. Material and methods: We transfected vector-only, wild-type, the Serine 100 to alanine (S100A) or to glutamic acid (S100E) substitution of Snail into various cell lines. We assessed colony formation, γ-H2AX focus formation and the invasion index in the cells treated with or without IR. Results: We found that over-expression of the S100A mutant Snail in HeLa cells significantly increased radiosensitivity. Meanwhile the expression of S100E, a phospho-mimicking mutation, resulted in enhanced radio-resistance. Interestingly, S100E could rescue the radiosensitive phenotype in ATM-deficient cells. We also found that expression of S100E increased γ-H2AX focus formation and compromised inhibition of invasion in response to IR independent of cell survival. Conclusion: ATM-mediated Snail Serine 100 phosphorylation in response to IR plays an important part in the regulation of radiosensitivity

The use of high-speed imaging in education

Science.gov (United States)

Kleine, H.; McNamara, G.; Rayner, J.

2017-02-01

Recent improvements in camera technology and the associated improved access to high-speed camera equipment have made it possible to use high-speed imaging not only in a research environment but also specifically for educational purposes. This includes high-speed sequences that are created both with and for a target audience of students in high schools and universities. The primary goal is to engage students in scientific exploration by providing them with a tool that allows them to see and measure otherwise inaccessible phenomena. High-speed imaging has the potential to stimulate students' curiosity as the results are often surprising or may contradict initial assumptions. "Live" demonstrations in class or student- run experiments are highly suitable to have a profound influence on student learning. Another aspect is the production of high-speed images for demonstration purposes. While some of the approaches known from the application of high speed imaging in a research environment can simply be transferred, additional techniques must often be developed to make the results more easily accessible for the targeted audience. This paper describes a range of student-centered activities that can be undertaken which demonstrate how student engagement and learning can be enhanced through the use of high speed imaging using readily available technologies.

Perspectives in high-speed transport: Maglev and/or wheel-and-rail techniques

Energy Technology Data Exchange (ETDEWEB)

Hochbruck, H

1985-03-01

Proceeding from a consideration of possible system structures, a technically and economically meaning-full top speed for a railway system is developed which offers, in competition with the airplane and the private car, good chances of winning an appropriate share of the traffic where the distances are in the range of about 400 to 600 km. It is deduced from the present state of developments in high-speed wheel/rail systems and in maglev technology that the wheel/rail mode has clear advantages where a network is concerned. Maglev technology is now being brought to the stage of operational readiness and its first applications are likely to be for point-to-point or direct-line links.

Base station MAC with APRMA protocol for broadband multimedia ATM in micro/pico-cellular mobile networks

DEFF Research Database (Denmark)

Le, Khanh Hoang; Nielsen, Søren Nørskov; Dittmann, Lars

1998-01-01

The concept for a wireless ATM access system that enables seamless mobile connectivity to the B-ISDN is presented. It is based on small, low cost and intelligent base stations running a medium access control (MAC) protocol using adaptive packet reservation multiple access (APRMA). Both...

ATM is required for SOD2 expression and homeostasis within the mammary gland.

Science.gov (United States)

Dyer, Lisa M; Kepple, Jessica D; Ai, Lingbao; Kim, Wan-Ju; Stanton, Virginia L; Reinhard, Mary K; Backman, Lindsey R F; Streitfeld, W Scott; Babu, Nivetha Ramesh; Treiber, Nicolai; Scharffetter-Kochanek, Karin; McKinnon, Peter J; Brown, Kevin D

2017-12-01

ATM activates the NF-κB transcriptional complex in response to genotoxic and oxidative stress. The purpose of this study was to examine if the NF-κB target gene and critical antioxidant SOD2 (MnSOD) in cultured mammary epithelium is also ATM-dependent, and what phenotypes arise from deletion of ATM and SOD2 within the mammary gland. SOD2 expression was studied in human mammary epithelial cells and MCF10A using RNAi to knockdown ATM or the NF-κB subunit RelA. To study ATM and SOD2 function in mammary glands, mouse lines containing Atm or Sod2 genes containing LoxP sites were mated with mice harboring Cre recombinase under the control of the whey acidic protein promoter. Quantitative PCR was used to measure gene expression, and mammary gland structure was studied using histology. SOD2 expression is ATM- and RelA-dependent, ATM knockdown renders cells sensitive to pro-oxidant exposure, and SOD mimetics partially rescue this sensitivity. Mice with germline deletion of Atm fail to develop mature mammary glands, but using a conditional knockout approach, we determined that Atm deletion significantly diminished the expression of Sod2. We also observed that these mice (termed Atm Δ/Δ ) displayed a progressive lactation defect as judged by reduced pup growth rate, aberrant lobulo-alveolar structure, diminished milk protein gene expression, and increased apoptosis within lactating glands. This phenotype appears to be linked to dysregulated Sod2 expression as mammary gland-specific deletion of Sod2 phenocopies defects observed in Atm Δ/Δ dams. We conclude that ATM is required to promote expression of SOD2 within the mammary epithelium, and that both ATM and SOD2 play a crucial role in mammary gland homeostasis.

High-speed elevators controlled by inverters

Energy Technology Data Exchange (ETDEWEB)

Sakai, Yoshio; Takahashi, Hideaki; Nakamura, Kiyoshi; Kinoshita, Hiroshi

1988-10-25

The super-high-speed elevator with superiority to 300m/min of speed, requires both the large capacity power and wide range speed controls. Therefore, in order to materialize the smooth and quiet operation characteristics, by applying the inverter control, the low torque ripple control in the low frequency range and high frequency large capacity inverting for lowering the motor in noise are necessary with their being assured of reliability. To satisfy the above necessary items, together with the development of a sine wave pulse width and frequency modulation (PWM/PFM) control system, to more precisely enable the sine wave electric current control, and 3kHz switching power converter, using a 800A power transistor module, a supervoltage control circuit under the extraordinary condition was designed. As a result of commercializing a 360m/min super-high speed inverter elevator, the power source unit, due to the effect of high power factor, could be reduced by 30% in capacity and also the higher harmonic wave including ratio could be considerably lowered to the inferiority to 5%. 2 references, 7 figures, 1 table.

Participation of ATM in cellular response to DNA damage induced by ionizing radiation

International Nuclear Information System (INIS)

Meng Xiangbing; Song Yi; Mao Jianping; Gong Bo; Dong Yan; Liu Bin; Sun Zhixian

2000-01-01

Objective: To clone ATM full length cDNA and cDNA fragments containing some functional domains and to identify proteins that interact with ATM and mediate DNA damage signal transduction in cellular response to DNA damage. Methods: ATM cDNA was amplified from MarthomTM-Ready cDNA kit of human leukocytes by LD-PCR. ATM-interacting proteins were screened by yeast two hybrid system. Results: ATM full-length cDNA and cDNA fragments containing PI3K kinase domain, leucine zipper and proline rich region were amplified from human cDNAs. Several candidate clones that interacted with ATM PI3K domain were identified. Conclusion: ATM mediates DNA damage signal transduction by interacting with many proteins

ATM Polymorphisms Are Associated With Risk of Radiation-Induced Pneumonitis

International Nuclear Information System (INIS)

Zhang Li; Yang Ming; Bi Nan; Fang Mingjing; Sun Tong; Ji Wei; Tan Wen; Zhao Lujun; Yu Dianke; Lin Dongxin; Wang Luhua

2010-01-01

Purpose: Since the ataxia telangiectasia mutated (ATM) protein plays crucial roles in repair of double-stranded DNA breaks, control of cell cycle checkpoints, and radiosensitivity, we hypothesized that variations in this gene might be associated with radiation-induced pneumonitis (RP). Methods and Materials: A total of 253 lung cancer patients receiving thoracic irradiation between 2004 and 2006 were included in this study. Common Terminology Criteria for Adverse Events version 3.0 was used to grade RP. Five haplotype-tagging single nucleotide polymorphisms (SNPs) in the ATM gene were genotyped using DNA from blood lymphocytes. Hazard ratios (HRs) and 95% confidence intervals (CIs) of RP for genotypes were computed by the Cox model, adjusted for clinical factors. The function of the ATM SNP associated with RP was examined by biochemical assays. Results: During the median 22-month follow-up, 44 (17.4%) patients developed grade ≥ 2 RP. In multivariate Cox regression models adjusted for other clinical predictors, we found two ATM variants were independently associated with increased RP risk. They were an 111G > A) polymorphism (HR, 2.49; 95% CI, 1.07-5.80) and an ATM 126713G > A polymorphism (HR, 2.47; 95% CI, 1.16-5.28). Furthermore, genotype-dependent differences in ATM expression were demonstrated both in cell lines (p < 0.001) and in individual lung tissue samples (p = 0.003), which supported the results of the association study. Conclusions: Genetic polymorphisms of ATM are significantly associated with RP risk. These variants might exert their effect through regulation of ATM expression and serve as independent biomarkers for prediction of RP in patients treated with thoracic radiotherapy.

High - speed steel for precise cased tools

International Nuclear Information System (INIS)

Karwiarz, J.; Mazur, A.

2001-01-01

The test results of high-vanadium high - speed steel (SWV9) for precise casted tools are presented. The face -milling cutters of NFCa80A type have been tested in industrial operating conditions. An average life - time of SWV9 steel tools was 3-10 times longer compare to the conventional high - speed milling cutters. Metallography of SWB9 precise casted steel revealed beneficial for tool properties distribution of primary vanadium carbides in the steel matrix. Presented results should be a good argument for wide application of high - vanadium high - speed steel for precise casted tools. (author)

Distributed medical services within the ATM-based Berlin regional test bed

Science.gov (United States)

Thiel, Andreas; Bernarding, Johannes; Krauss, Manfred; Schulz, Sandra; Tolxdorff, Thomas

1996-05-01

The ATM-based Metropolitan Area Network (MAN) of Berlin connects two university hospitals (Benjamin Franklin University Hospital and Charite) with the computer resources of the Technical University of Berlin (TUB). Distributed new medical services have been implemented and will be evaluated within the highspeed MAN of Berlin. The network with its data transmission rates of up to 155 Mbit/s renders these medical services externally available to practicing physicians. Resource and application sharing is demonstrated by the use of two software systems. The first software system is an interactive 3D reconstruction tool (3D- Medbild), based on a client-server mechanism. This structure allows the use of high- performance computers at the TUB from the low-level workstations in the hospitals. A second software system, RAMSES, utilizes a tissue database of Magnetic Resonance Images. For the remote control of the software, the developed applications use standards such as DICOM 3.0 and features of the World Wide Web. Data security concepts are being tested and integrated for the needs of the sensitive medical data. The highspeed network is the necessary prerequisite for the clinical evaluation of data in a joint teleconference. The transmission of digitized real-time sequences such as video and ultrasound and the interactive manipulation of data are made possible by Multi Media tools.

Loss of ATM kinase activity leads to embryonic lethality in mice.

Science.gov (United States)

Daniel, Jeremy A; Pellegrini, Manuela; Lee, Baeck-Seung; Guo, Zhi; Filsuf, Darius; Belkina, Natalya V; You, Zhongsheng; Paull, Tanya T; Sleckman, Barry P; Feigenbaum, Lionel; Nussenzweig, André

2012-08-06

Ataxia telangiectasia (A-T) mutated (ATM) is a key deoxyribonucleic acid (DNA) damage signaling kinase that regulates DNA repair, cell cycle checkpoints, and apoptosis. The majority of patients with A-T, a cancer-prone neurodegenerative disease, present with null mutations in Atm. To determine whether the functions of ATM are mediated solely by its kinase activity, we generated two mouse models containing single, catalytically inactivating point mutations in Atm. In this paper, we show that, in contrast to Atm-null mice, both D2899A and Q2740P mutations cause early embryonic lethality in mice, without displaying dominant-negative interfering activity. Using conditional deletion, we find that the D2899A mutation in adult mice behaves largely similar to Atm-null cells but shows greater deficiency in homologous recombination (HR) as measured by hypersensitivity to poly (adenosine diphosphate-ribose) polymerase inhibition and increased genomic instability. These results may explain why missense mutations with no detectable kinase activity are rarely found in patients with classical A-T. We propose that ATM kinase-inactive missense mutations, unless otherwise compensated for, interfere with HR during embryogenesis.

New Architecture of Optical Interconnect for High-Speed Optical Computerized Data Networks (Nonlinear Response

Directory of Open Access Journals (Sweden)

El-Sayed A. El-Badawy

2008-02-01

Full Text Available Although research into the use of optics in computers has increased in the last and current decades, the fact remains that electronics is still superior to optics in almost every way. Research into the use of optics at this stage mirrors the research into electronics after the 2nd World War. The advantages of using fiber optics over wiring are the same as the argument for using optics over electronics in computers. Even through totally optical computers are now a reality, computers that combine both electronics and optics, electro-optic hybrids, have been in use for some time. In the present paper, architecture of optical interconnect is built up on the bases of four Vertical-Cavity Surface- Emitting Laser Diodes (VCSELD and two optical links where thermal effects of both the diodes and the links are included. Nonlinear relations are correlated to investigate the power-current and the voltage-current dependences of the four devices. The good performance (high speed of the interconnect is deeply and parametrically investigated under wide ranges of the affecting parameters. The high speed performance is processed through three different effects, namely the device 3-dB bandwidth, the link dispersion characteristics, and the transmitted bit rate (soliton. Eight combinations are investigated; each possesses its own characteristics. The best architecture is the one composed of VCSELD that operates at 850 nm and the silica fiber whatever the operating set of causes. This combination possesses the largest device 3-dB bandwidth, the largest link bandwidth and the largest soliton transmitted bit rate. The increase of the ambient temperature reduces the high-speed performance of the interconnect

In situ health monitoring for bogie systems of CRH380 train on Beijing-Shanghai high-speed railway

Science.gov (United States)

Hong, Ming; Wang, Qiang; Su, Zhongqing; Cheng, Li

2014-04-01

Based on the authors' research efforts over the years, an in situ structural health monitoring (SHM) technique taking advantage of guided elastic waves has been developed and deployed via an online diagnosis system. The technique and the system were recently implemented on China's latest high-speed train (CRH380CL) operated on Beijing-Shanghai High-Speed Railway. The system incorporated modularized components including active sensor network, active wave generation, multi-channel data acquisition, signal processing, data fusion, and results presentation. The sensor network, inspired by a new concept—"decentralized standard sensing", was integrated into the bogie frames during the final assembly of CRH380CL, to generate and acquire bogie-guided ultrasonic waves, from which a wide array of signal features were extracted. Fusion of signal features through a diagnostic imaging algorithm led to a graphic illustration of the overall health state of the bogie in a real-time and intuitive manner. The in situ experimentation covered a variety of high-speed train operation events including startup, acceleration/deceleration, full-speed operation (300 km/h), emergency braking, track change, as well as full stop. Mock-up damage affixed to the bogie was identified quantitatively and visualized in images. This in situ testing has demonstrated the feasibility, effectiveness, sensitivity, and reliability of the developed SHM technique and the system towards real-world applications.

Phosphorylation of p300 by ATM controls the stability of NBS1

International Nuclear Information System (INIS)

Jang, Eun Ryoung; Choi, Jae Duk; Jeong, Gajin; Lee, Jong-Soo

2010-01-01

Acetyltransferase, p300 is a transcriptional cofactor of signal-responsive transcriptional regulation. The surveillance kinase ataxia-telangiectasia mutated (ATM) plays a central role in regulation of a wide range of cellular DNA damage responses. Here, we investigated whether and how ATM mediates phosphorylation of p300 in response to DNA damage and how p300 phosphorylation is functionally linked to DNA damage. ATM-phosphorylated p300 in vitro and in vivo, in response to DNA damage. Phosphorylation of p300 proteins was observed upon γ-irradiation in ATM + cells but not ATM - cells. Importantly, expression of nonphosphorylatable serine to alanine form of p300 (S106A) destabilized both p300 and NBS1 proteins, after DNA damage. These data demonstrate that ATM transduces a DNA damage signal to p300, and that ATM-dependent phosphorylation of p300 is required for stabilization of NBS1 proteins in response to DNA damage.

ATM regulates Cdt1 stability during the unperturbed S phase to prevent re-replication

Science.gov (United States)

Iwahori, Satoko; Kohmon, Daisuke; Kobayashi, Junya; Tani, Yuhei; Yugawa, Takashi; Komatsu, Kenshi; Kiyono, Tohru; Sugimoto, Nozomi; Fujita, Masatoshi

2014-01-01

Ataxia-telangiectasia mutated (ATM) plays crucial roles in DNA damage responses, especially with regard to DNA double-strand breaks (DSBs). However, it appears that ATM can be activated not only by DSB, but also by some changes in chromatin architecture, suggesting potential ATM function in cell cycle control. Here, we found that ATM is involved in timely degradation of Cdt1, a critical replication licensing factor, during the unperturbed S phase. At least in certain cell types, degradation of p27Kip1 was also impaired by ATM inhibition. The novel ATM function for Cdt1 regulation was dependent on its kinase activity and NBS1. Indeed, we found that ATM is moderately phosphorylated at Ser1981 during the S phase. ATM silencing induced partial reduction in levels of Skp2, a component of SCFSkp2 ubiquitin ligase that controls Cdt1 degradation. Furthermore, Skp2 silencing resulted in Cdt1 stabilization like ATM inhibition. In addition, as reported previously, ATM silencing partially prevented Akt phosphorylation at Ser473, indicative of its activation, and Akt inhibition led to modest stabilization of Cdt1. Therefore, the ATM-Akt-SCFSkp2 pathway may partly contribute to the novel ATM function. Finally, ATM inhibition rendered cells hypersensitive to induction of re-replication, indicating importance for maintenance of genome stability. PMID:24280901

NEBULAS A High Performance Data-Driven Event-Building Architecture based on an Asynchronous Self-Routing Packet-Switching Network

CERN Multimedia

Costa, M; Letheren, M; Djidi, K; Gustafsson, L; Lazraq, T; Minerskjold, M; Tenhunen, H; Manabe, A; Nomachi, M; Watase, Y

2002-01-01

RD31 : The project is evaluating a new approach to event building for level-two and level-three processor farms at high rate experiments. It is based on the use of commercial switching fabrics to replace the traditional bus-based architectures used in most previous data acquisition sytems. Switching fabrics permit the construction of parallel, expandable, hardware-driven event builders that can deliver higher aggregate throughput than the bus-based architectures. A standard industrial switching fabric technology is being evaluated. It is based on Asynchronous Transfer Mode (ATM) packet-switching network technology. Commercial, expandable ATM switching fabrics and processor interfaces, now being developed for the future Broadband ISDN infrastructure, could form the basis of an implementation. The goals of the project are to demonstrate the viability of this approach, to evaluate the trade-offs involved in make versus buy options, to study the interfacing of the physics frontend data buffers to such a fabric, a...

High-speed ground transportation development outside United States

Energy Technology Data Exchange (ETDEWEB)

Eastham, T.R. [Queen`s Univ., Kingston, Ontario (United Kingdom)

1995-09-01

This paper surveys the state of high-speed (in excess of 200 km/h) ground-transportation developments outside the United States. Both high-speed rail and Maglev systems are covered. Many vehicle systems capable of providing intercity service in the speed range 200--500 km/h are or will soon be available. The current state of various technologies, their implementation, and the near-term plans of countries that are most active in high-speed ground transportation development are reported.

Aerodynamic design on high-speed trains

Science.gov (United States)

Ding, San-San; Li, Qiang; Tian, Ai-Qin; Du, Jian; Liu, Jia-Li

2016-04-01

Compared with the traditional train, the operational speed of the high-speed train has largely improved, and the dynamic environment of the train has changed from one of mechanical domination to one of aerodynamic domination. The aerodynamic problem has become the key technological challenge of high-speed trains and significantly affects the economy, environment, safety, and comfort. In this paper, the relationships among the aerodynamic design principle, aerodynamic performance indexes, and design variables are first studied, and the research methods of train aerodynamics are proposed, including numerical simulation, a reduced-scale test, and a full-scale test. Technological schemes of train aerodynamics involve the optimization design of the streamlined head and the smooth design of the body surface. Optimization design of the streamlined head includes conception design, project design, numerical simulation, and a reduced-scale test. Smooth design of the body surface is mainly used for the key parts, such as electric-current collecting system, wheel truck compartment, and windshield. The aerodynamic design method established in this paper has been successfully applied to various high-speed trains (CRH380A, CRH380AM, CRH6, CRH2G, and the Standard electric multiple unit (EMU)) that have met expected design objectives. The research results can provide an effective guideline for the aerodynamic design of high-speed trains.

ATM and checkpoint responses to DNA double strand breaks

International Nuclear Information System (INIS)

Khanna, K.K.

2003-01-01

DNA damage checkpoints can be classified into G1/S, intra-S and G2/M checkpoints, so named according to the cell cycle transitions that they regulate. DNA damage incurred during the G1 or G2 phase of the cell cycle leads to growth arrest at the G1/S and G2/M phase boundaries, respectively, whereas genotoxic stress during S phase results in the transient suppression of DNA synthesis. In mammals, ATM (ataxia-telangiectasia mutated) is a protein kinase that controls all checkpoint responses to DNA damage. ATM is a versatile kinase which uses various means to regulate a given checkpoint pathway. It has been shown to act upon several proteins within the same pathway, many times controlling several different modifications of the same protein or using several different targets to arrive at the same end point. Some of the ATM targets act as adaptors by recruiting additional substrates for ATM. ATM controls two types of responses in G1. The p53-dependent responses inhibit Cyclin/Cdk activity by transcriptional induction of p21, whereas p53-independent responses inhibit CDKs through degradation of Cdc25A to maintain CdK2 inhibitory phosphorylation. In regulating p53, ATM directly phosphorylates p53 on Ser15, which likely causes p53 transcriptional activation, concurrently activating other kinases that phosphorylate p53 at other sites such as Ser20, which reduces the ability of MDM2 to bind p53, thus promoting its stability. ATM further ensures p53 stability by phosphorylating MDM2. At least six ATM targets, namely CHK2, CHK1, NBS1, BRCA1, SMC1 and FANCD2, have been implicated in the control of S-phase checkpoint. Cdc25A is the downstream effector of CHK1 and CHK2, though the underlying mechanism for control of intra S-phase checkpoint by other targets remain obscure. G2 checkpoint prevents mitotic entry solely through inhibitory phosphorylation of Cdc2/Cdk1. Several ATM targets including CHK1, CHK2, BRCA1, MDC1 and p53BP1 have been implicated in the control of G2/M

Firewall Architectures for High-Speed Networks: Final Report

Energy Technology Data Exchange (ETDEWEB)

Errin W. Fulp

2007-08-20

Firewalls are a key component for securing networks that are vital to government agencies and private industry. They enforce a security policy by inspecting and filtering traffic arriving or departing from a secure network. While performing these critical security operations, firewalls must act transparent to legitimate users, with little or no effect on the perceived network performance (QoS). Packets must be inspected and compared against increasingly complex rule sets and tables, which is a time-consuming process. As a result, current firewall systems can introduce significant delays and are unable to maintain QoS guarantees. Furthermore, firewalls are susceptible to Denial of Service (DoS) attacks that merely overload/saturate the firewall with illegitimate traffic. Current firewall technology only offers a short-term solution that is not scalable; therefore, the \\textbf{objective of this DOE project was to develop new firewall optimization techniques and architectures} that meet these important challenges. Firewall optimization concerns decreasing the number of comparisons required per packet, which reduces processing time and delay. This is done by reorganizing policy rules via special sorting techniques that maintain the original policy integrity. This research is important since it applies to current and future firewall systems. Another method for increasing firewall performance is with new firewall designs. The architectures under investigation consist of multiple firewalls that collectively enforce a security policy. Our innovative distributed systems quickly divide traffic across different levels based on perceived threat, allowing traffic to be processed in parallel (beyond current firewall sandwich technology). Traffic deemed safe is transmitted to the secure network, while remaining traffic is forwarded to lower levels for further examination. The result of this divide-and-conquer strategy is lower delays for legitimate traffic, higher throughput

The mammalian mid-pachytene checkpoint: meiotic arrest in spermatocytes with a mutation in Atm alone or in combination with a Trp53 (p53) or Cdkn1a (p21/cip1) mutation

NARCIS (Netherlands)

Ashley, T.; Westphal, C.; Plug-de Maggio, A.; de rooij, D. G.

2004-01-01

ATM, the protein product of the gene mutated in the human autosomal recessive disorder ataxia telangiectasia, is involved in detection of double strand breaks (DSBs) and is a key component of the damage surveillance network of cell cycle proteins. In somatic cells ATM phosphorylates many other

Molecular and functional characterization of a human ATM gene analogue at Arabidopsis thaliana

International Nuclear Information System (INIS)

Garcia, V.

2001-12-01

The human ATM gene, whose inactivation is responsible for the human disease ataxia telangiectasia is conserved throughout the Eukaryotes and plays an important role in the cellular responses to DNA damage, in particular to DNA double-strand breaks (DSBs). Here we describe the identification of an Arabidopsis thaliana homologue of ATM (AtATM), and the molecular and cytological characterization of plants, hereafter called atm, carrying a disrupting T-DNA insertion in this gene. AtATM covers a 32 kb region on chromosome 3. The AtATM transcript has a complex structure, is 12 kb long and formed by 79 exons. The transcriptional level of AtATM is very low in all the tissues tested, and does not vary after exposure to ionizing radiations (IR). In atm plants, the protein is not detected suggesting the mutants are null. The atm mutants are partially sterile. Aberrant segregation of chromosomes during meiosis I on both male and female sides account for this sterility. However, meiotic recombination frequency is normal. Mutant plants are also hypersensitive to gamma rays and methyl methane sulfonate, but not to UV-B, pointing to a specific defect of atm mutants in the response to DNA DSBs. In plants, ionizing radiations induce a strong, rapid and transient transcriptional activation of genes involved in the cellular response to or the repair of DSBs. This transcriptional regulation of AtRAD51, AtPARP1, atGR1 and AtL1G4 is lost in the atm mutants . The absence of AtRAD51 induction associated with ionizing radiation sensitivity suggest that AtAtm play an important function in DSB repair by homologous recombination. In addition we show that homologous intra-chromosomal recombination frequency is elevated in the mutant comparing to wild-type, with or without gamma irradiation. These results show the implication of AtAtm in the genomic stability. (author)

Functional analyses of ATM, ATR and Fanconi anemia proteins in lung carcinoma

International Nuclear Information System (INIS)

Beumer, Jan H.; Fu, Katherine Y.; Anyang, Bean N.; Siegfried, Jill M.; Bakkenist, Christopher J.

2015-01-01

ATM and ATR are kinases implicated in a myriad of DNA-damage responses. ATM kinase inhibition radiosensitizes cells and selectively kills cells with Fanconi anemia (FA) gene mutations. ATR kinase inhibition sensitizes cells to agents that induce replication stress and selectively kills cells with ATM and TP53 mutations. ATM mutations and FANCF promoter-methylation are reported in lung carcinomas. We undertook functional analyses of ATM, ATR, Chk1 and FA proteins in lung cancer cell lines. We included Calu6 that is reported to be FANCL-deficient. In addition, the cancer genome atlas (TCGA) database was interrogated for alterations in: 1) ATM, MRE11A, RAD50 and NBN; 2) ATR, ATRIP and TOPBP1; and 3) 15 FA genes. No defects in ATM, ATR or Chk1 kinase activation, or FANCD2 monoubiquitination were identified in the lung cancer cell lines examined, including Calu6, and major alterations in these pathways were not identified in the TCGA database. Cell lines were radiosensitized by ATM kinase inhibitor KU60019, but no cell killing by ATM kinase inhibitor alone was observed. While no synergy between gemcitabine or carboplatin and ATR kinase inhibitor ETP-46464 was observed, synergy between gemcitabine and Chk1 kinase inhibitor UCN-01 was observed in 54 T, 201 T and H460, and synergy between carboplatin and Chk1 kinase inhibitor was identified in 201 T and 239 T. No interactions between ATM, ATR and FA activation were observed by either ATM or ATR kinase inhibition in the lung cancer cell lines. Analyses of ATM serine 1981 and Chk1 serine 345 phosphorylation, and FANCD2 monoubiquitination revealed that ATM and ATR kinase activation and FA pathway signaling are intact in the lung cancer cell lines examined. As such, these posttranslational modifications may have utility as biomarkers for the integrity of DNA damage signaling pathways in lung cancer. Different sensitization profiles between gemcitabine and carboplatin and ATR kinase inhibitor ETP-46464 and Chk1 kinase inhibitor

Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress

Directory of Open Access Journals (Sweden)

Stephanie Munk

2017-10-01

Full Text Available The mechanisms that protect eukaryotic DNA during the cumbersome task of replication depend on the precise coordination of several post-translational modification (PTM-based signaling networks. Phosphorylation is a well-known regulator of the replication stress response, and recently an essential role for SUMOs (small ubiquitin-like modifiers has also been established. Here, we investigate the global interplay between phosphorylation and SUMOylation in response to replication stress. Using SUMO and phosphoproteomic technologies, we identify thousands of regulated modification sites. We find co-regulation of central DNA damage and replication stress responders, of which the ATR-activating factor TOPBP1 is the most highly regulated. Using pharmacological inhibition of the DNA damage response kinases ATR and ATM, we find that these factors regulate global protein SUMOylation in the protein networks that protect DNA upon replication stress and fork breakage, pointing to integration between phosphorylation and SUMOylation in the cellular systems that protect DNA integrity.

Development of the Advanced Technology Microwave Sounder (ATMS) for NPOESS C1

Science.gov (United States)

Brann, C.; Kunkee, D.

2008-12-01

The National Polar-orbiting Operational Environmental Satellite System's Advanced Technology Microwave Sounder (ATMS) is planned for flight on the first NPOESS mission (C1) in 2013. The C1 ATMS will be the second instrument of the ATMS series and will provide along with the companion Cross-track Infrared Sounder (CrIS), atmospheric temperature and moisture profiles for NPOESS. The first flight of the ATMS is scheduled in 2010 on the NPOESS Preparatory Project (NPP) satellite, which is an early instrument risk reduction component of the NPOESS mission. This poster will focus on the development of the ATMS for C1 including aspects of the sensor calibration, antenna beam and RF characteristics and scanning. New design aspects of the C1 ATMS, required primarily by parts obsolescence, will also be addressed in this poster.

Turbulent flame speeds and NOx kinetics of HHC fuels with contaminants and high dilution levels

Energy Technology Data Exchange (ETDEWEB)

Petersen, Eric; Krejci, Michael; Mathieu, Olivier; Vissotski, Andrew; Ravi, Sankar; Plichta, Drew; Sikes, Travis; Levacque, Anthony; Aul, Christopher; Petersen, Eric

2012-09-30

This progress report documents the second year of the project, from October 1, 2011 through September 30, 2012. Characterization of the new turbulent flame speed vessel design was completed. Turbulence statistics of three impellers with different geometric features were measured using particle image velocimetry inside a Plexiglas model (~1:1 scale) of a cylindrical flame speed vessel (30.5 cm ID × 35.6 cm L). With four impellers arranged in a central-symmetric configuration, turbulence intensities between 1.2 and 1.7 m/s with negligible mean flow (0.1u´) were attained at the lowest fan speeds. Acceptable ranges for homogeneity and isotropy ratios of the velocity fields were set within a narrow bandwidth near unity (0.9-1.1). Homogeneity ratios were unaffected by changes to the impeller geometry, and the prototype with the higher number of blades caused the flow to become anisotropic. The integral length scale of the flow fields varied between 27 and 20 mm, which correlates well with those typically observed inside a gas turbine combustor. The mechanism to independently vary the intensity level and the integral length scale was established, where turbulence intensity level was dependent on the rotational speed of the fan, and the integral length scale decreased with increasing blade pitch angle. Ignition delay times of H₂/O₂ mixtures highly diluted with Ar and doped with various amounts of N₂O (100, 400, 1600, 3200 ppm) were measured in a shock tube behind reflected shock waves over a wide range of temperatures (940-1675 K). The pressure range investigated during this work (around 1.6, 13, and 30 atm) allows studying the effect of N₂O on hydrogen ignition at pressure conditions that have never been heretofore investigated. Ignition delay times were decreased when N₂O was added to the mixture only for the higher nitrous oxide concentrations, and some changes in the activation energy were also observed at 1.5 and 30 atm. When it occurred, the decrease in

AN ANALYSIS OF SPATIAL EQUITY CONCERNING INVESTMENTS IN HIGH-SPEED RAIL SYSTEMS: THE CASE STUDY OF ITALY

Directory of Open Access Journals (Sweden)

Francesca PAGLIARA

2016-09-01

Full Text Available It is recognised in the literature that spatial accessibility is a measure of spatial equity and can be represented by the ease of travelling from an origin to a given destination via a given mode or set of transport modes. Although urban areas can benefit from improvements in accessibility when a new high-speed rail line is built, equity issues may arise. This manuscript describes a methodology for evaluating equity impacts due to an extension of the High Speed Rail network in Italy. A joint Revealed/Stated Preference survey has been carried out, collecting socioeconomic and travel data. Specifically, nine hypothetical scenarios have been submitted to Italian users aiming at understanding the motivations for not choosing the High Speed Rail as an alternative. The main outcome is that the access/egress travel costs connected with the High Speed Rail have a strong impact on spatial equity. The main policy implications of this study are that investors in high-speed rail should not only take into account the economic benefits brought by them, but also the spatial imbalance that these systems can bring.

Loss of ATM kinase activity leads to embryonic lethality in mice

DEFF Research Database (Denmark)

Daniel, J.A.; Pellegrini, M.; Filsuf, D.

2012-01-01

whether the functions of ATM are mediated solely by its kinase activity, we generated two mouse models containing single, catalytically inactivating point mutations in Atm. In this paper, we show that, in contrast to Atm-null mice, both D2899A and Q2740P mutations cause early embryonic lethality in mice......, without displaying dominant-negative interfering activity. Using conditional deletion, we find that the D2899A mutation in adult mice behaves largely similar to Atm-null cells but shows greater deficiency in homologous recombination (HR) as measured by hypersensitivity to poly (adenosine diphosphate...

Design of a QoS-controlled ATM-based communications system in chorus

Science.gov (United States)

Coulson, Geoff; Campbell, Andrew; Robin, Philippe; Blair, Gordon; Papathomas, Michael; Shepherd, Doug

1995-05-01

We describe the design of an application platform able to run distributed real-time and multimedia applications alongside conventional UNIX programs. The platform is embedded in a microkernel/PC environment and supported by an ATM-based, QoS-driven communications stack. In particular, we focus on resource-management aspects of the design and deal with CPU scheduling, network resource-management and memory-management issues. An architecture is presented that guarantees QoS levels of both communications and processing with varying degrees of commitment as specified by user-level QoS parameters. The architecture uses admission tests to determine whether or not new activities can be accepted and includes modules to translate user-level QoS parameters into representations usable by the scheduling, network, and memory-management subsystems.

Sensorless Speed/Torque Control of DC Machine Using Artificial Neural Network Technique

Directory of Open Access Journals (Sweden)

Rakan Kh. Antar

2017-12-01

Full Text Available In this paper, Artificial Neural Network (ANN technique is implemented to improve speed and torque control of a separately excited DC machine drive. The speed and torque sensorless scheme based on ANN is estimated adaptively. The proposed controller is designed to estimate rotor speed and mechanical load torque as a Model Reference Adaptive System (MRAS method for DC machine. The DC drive system consists of four quadrant DC/DC chopper with MOSFET transistors, ANN, logic gates and routing circuits. The DC drive circuit is designed, evaluated and modeled by Matlab/Simulink in the forward and reverse operation modes as a motor and generator, respectively. The DC drive system is simulated at different speed values (±1200 rpm and mechanical torque (±7 N.m in steady state and dynamic conditions. The simulation results illustratethe effectiveness of the proposed controller without speed or torque sensors.

Asynchronous transfer mode and Local Area Network emulation standards, protocols, and security implications

OpenAIRE

Kirwin, John P.

1999-01-01

A complex networking technology called Asynchronous Transfer Mode (ATM) and a networking protocol called Local Area Network Emulation (LANE) are being integrated into many naval networks without any security-driven naval configuration guidelines. No single publication is available that describes security issues of data delivery and signaling relating to the transition of Ethernet to LANE and ATM. The thesis' focus is to provide: (1) an overview and security analysis of standardized protocols ...

A Methodology to Integrate Security and Cost-effectiveness in ATM

OpenAIRE

Matarese, Francesca; Montefusco, Patrizia; Neves, José; Rocha, André

2014-01-01

The objective of this paper is the definition of a new methodology for carrying out security risk assessment in the air traffic management (ATM) domain so as to enhance security awareness and integrate secure and cost-effective design objectives. This process is carried out by modelling the system, identifying the assets, threats and vulnerabilities, prioritizing the threats and proposing cost-effective countermeasures for the weaknesses found. ATM security is concerned with securing ATM a...

Complex control of ATM in response to radiation damage to DNA

International Nuclear Information System (INIS)

Lavin, M.F.; Beamish, H.; Chen, P.; Keating, K.; Scott, S.; Spring, K.; Kozlov, S.; Walters, D.

2000-01-01

Full text: The human genetic disorder ataxia-telangiectasia is characterized by neurodegeneration, immunodeficiency, extreme sensitivity to ionizing radiation, abnormalities in cell cycle checkpoints and a predisposition to develop leukemias and lymphomas. It appears likely that the basis of the hypersensitivity to ionizing radiation is due to defective sensing of double strand breaks in DNA and as a consequence a failure to repair all of these breaks. After exposure of cells to radiation the kinase activity of pre-existing ATM protein is rapidly activated leading to the radiation-induced phosphoylation of a number of important substrates including p53, c-Abl, BRCA1, NBS1 and chk2. Defective phosphorylation of BRCA1 and NBS1 is associated with increased sensitivity to ionizing radiation. We have also demonstrated that a reduction in the amount of ATM protein using antisense ATM cDNA transfection prior to exposure to radiation also sensitizes cells. This was further confirmed by treating human lymphoblastoid cells with EGF prior to radiation exposure. Furthermore radiation reverses the downregulation of ATM by EGF over a 3 hour period. Under these conditions cells are still sensitized to radiation since the restoration of ATM kinase activity is slower than that arising from activation of existing protein. Alterations in the amount of ATM protein are also observed in response to mitogenic agents. Thus it is evident that ATM protein and kinase activity are regulated in a complex fashion and this appears to vary in different tissues. The implications for altering ATM for therapeutic benefit will be discussed

The Kaleidoscope switch-a new concept for implementation of a large and fault tolerant ATM switch system

DEFF Research Database (Denmark)

Dittmann, Lars

1997-01-01

This paper describes a new concept for implementing a large switch network based on smaller modules. The concept is based an an alternative self-routing structure that due to a point symmetry allows the bit in the routing tag to be processed in a random order. Among others this property provides ...... an inherent fault protection and allows a simple implementation of broadcast and multicast. The concept has been implemented as a small prototype, that currently is used in a national experimental ATM network in Denmark...

Full-frame, high-speed 3D shape and deformation measurements using stereo-digital image correlation and a single color high-speed camera

Science.gov (United States)

Yu, Liping; Pan, Bing

2017-08-01

Full-frame, high-speed 3D shape and deformation measurement using stereo-digital image correlation (stereo-DIC) technique and a single high-speed color camera is proposed. With the aid of a skillfully designed pseudo stereo-imaging apparatus, color images of a test object surface, composed of blue and red channel images from two different optical paths, are recorded by a high-speed color CMOS camera. The recorded color images can be separated into red and blue channel sub-images using a simple but effective color crosstalk correction method. These separated blue and red channel sub-images are processed by regular stereo-DIC method to retrieve full-field 3D shape and deformation on the test object surface. Compared with existing two-camera high-speed stereo-DIC or four-mirror-adapter-assisted singe-camera high-speed stereo-DIC, the proposed single-camera high-speed stereo-DIC technique offers prominent advantages of full-frame measurements using a single high-speed camera but without sacrificing its spatial resolution. Two real experiments, including shape measurement of a curved surface and vibration measurement of a Chinese double-side drum, demonstrated the effectiveness and accuracy of the proposed technique.

Development of a model and test equipment for cold flow tests at 500 atm of small nuclear light bulb configurations

Science.gov (United States)

Jaminet, J. F.

1972-01-01

A model and test equipment were developed and cold-flow-tested at greater than 500 atm in preparation for future high-pressure rf plasma experiments and in-reactor tests with small nuclear light bulb configurations. With minor exceptions, the model chamber is similar in design and dimensions to a proposed in-reactor geometry for tests with fissioning uranium plasmas in the nuclear furnace. The model and the equipment were designed for use with the UARL 1.2-MW rf induction heater in tests with rf plasmas at pressures up to 500 atm. A series of cold-flow tests of the model was then conducted at pressures up to about 510 atm. At 504 atm, the flow rates of argon and cooling water were 3.35 liter/sec (STP) and 26 gal/min, respectively. It was demonstrated that the model is capable of being operated for extended periods at the 500-atm pressure level and is, therefore, ready for use in initial high-pressure rf plasma experiments.

The over expression of long non-coding RNA ANRIL promotes epithelial-mesenchymal transition by activating the ATM-E2F1 signaling pathway in pancreatic cancer: An in vivo and in vitro study.

Science.gov (United States)

Chen, Shi; Zhang, Jia-Qiang; Chen, Jiang-Zhi; Chen, Hui-Xing; Qiu, Fu-Nan; Yan, Mao-Lin; Chen, Yan-Ling; Peng, Cheng-Hong; Tian, Yi-Feng; Wang, Yao-Dong

2017-09-01

This study aims to investigate the roles of lncRNA ANRIL in epithelial-mesenchymal transition (EMT) by regulating the ATM-E2F1 signaling pathway in pancreatic cancer (PC). PC rat models were established and ANRIL overexpression and interference plasmids were transfected. The expression of ANRIL, EMT markers (E-cadherin, N-cadherin and Vimentin) and ATM-E2F1 signaling pathway-related proteins (ATM, E2F1, INK4A, INK4B and ARF) were detected. Small molecule drugs were applied to activate and inhibit the ATM-E2F1 signaling pathway. Transwell assay and the scratch test were adopted to detect cell invasion and migration abilities. ANRIL expression in the PC cells was higher than in normal pancreatic duct epithelial cells. In the PC rat models and PC cells, ANRIL interference promoted the expressions of INK4B, INK4A, ARF and E-cadherin, while reduced N-cadherin and Vimentin expression. Over-expressed ANRIL decreased the expression of INK4B, INK4A, ARF and E-cadherin, but raised N-cadherin and Vimentin expressions. By inhibiting the ATM-E2F1 signaling pathway in PC cells, E-cadherin expression increased but N-cadherin and Vimentin expressions decreased. After ANRIL was silenced or the ATM-E2F1 signaling pathway inhibited, PC cell migration and invasion abilities were decreased. In conclusion, over-expression of lncRNA ANRIL can promote EMT of PC cells by activating the ATM-E2F1 signaling pathway. Copyright © 2017 Elsevier B.V. All rights reserved.

A distributed high speed data acquisition system for KT5C tokamak

International Nuclear Information System (INIS)

Sun Xiang; Wang Zhijiang; Lu Ronghua; Wang Jun; Yu Yi; Zhu Zhenghua; Wen Yizhi; Wan Shude; Liu Wandong; Yu Changxuan

2005-01-01

The development of a distributed data acquisition system with low cost to implement high speed data collection through the campus networks for a small tokamak, KT5C, is presented. Data of 512 k bytes at 5 MHz from 5 channels for each can be collected during about 10s after three researchers at different positions demand this system for acquisitions. This system realizes long distance multiuser operations; virtually efficiency of the data acquisition is enhanced. (authors)

Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway.

Science.gov (United States)

Gu, Xueyan; Qi, Yongmei; Feng, Zengxiu; Ma, Lin; Gao, Ke; Zhang, Yingmei

2018-07-01

Lead (Pb), a widely distributed environmental pollutant, is known to induce mitochondrial damage as well as autophagy in vitro and in vivo. In this study, we found that Pb could trigger mitophagy in both HEK293 cells and the kidney cortex of male Kunming mice. However, whether ataxia telangiectasis mutated (ATM) which is reported to be linked with PTEN-induced putative kinase 1 (PINK1)/Parkin pathway (a well-characterized mitophagic pathway) participates in the regulation of Pb-induced mitophagy and its exact role remains enigmatic. Our results indicated that Pb activated ATM in vitro and in vivo, and further in vitro studies showed that ATM could co-localize with PINK1 and Parkin in cytosol and interact with PINK1. Knockdown of ATM by siRNA blocked Pb-induced mitophagy even under the circumstance of enhanced accumulation of PINK1 and mitochondrial Parkin. Intriguingly, elevation instead of reduction in phosphorylation level of PINK1 and Parkin was observed in response to ATM knockdown and Pb did not contribute to the further increase of their phosphorylation level, implying that ATM indirectly regulated PINK1/Parkin pathway. These findings reveal a novel mechanism for Pb toxicity and suggest the regulatory importance of ATM in PINK1/Parkin-mediated mitophagy. Copyright © 2018 Elsevier B.V. All rights reserved.

Structures of closed and open conformations of dimeric human ATM

Science.gov (United States)

Baretić, Domagoj; Pollard, Hannah K.; Fisher, David I.; Johnson, Christopher M.; Santhanam, Balaji; Truman, Caroline M.; Kouba, Tomas; Fersht, Alan R.; Phillips, Christopher; Williams, Roger L.

2017-01-01

ATM (ataxia-telangiectasia mutated) is a phosphatidylinositol 3-kinase–related protein kinase (PIKK) best known for its role in DNA damage response. ATM also functions in oxidative stress response, insulin signaling, and neurogenesis. Our electron cryomicroscopy (cryo-EM) suggests that human ATM is in a dynamic equilibrium between closed and open dimers. In the closed state, the PIKK regulatory domain blocks the peptide substrate–binding site, suggesting that this conformation may represent an inactive or basally active enzyme. The active site is held in this closed conformation by interaction with a long helical hairpin in the TRD3 (tetratricopeptide repeats domain 3) domain of the symmetry-related molecule. The open dimer has two protomers with only a limited contact interface, and it lacks the intermolecular interactions that block the peptide-binding site in the closed dimer. This suggests that the open conformation may be more active. The ATM structure shows the detailed topology of the regulator-interacting N-terminal helical solenoid. The ATM conformational dynamics shown by the structures represent an important step in understanding the enzyme regulation. PMID:28508083

Design of an Electro-Optic Modulator for High Speed Communications

Science.gov (United States)

Espinoza, David

The telecommunications and computer technology industries have been requiring higher communications speeds at all levels for devices, components and interconnected systems. Optical devices and optical interconnections are a viable alternative over other traditional technologies such as copper-based interconnections. Latency reductions can be achieved through the use of optical interconnections. Currently, a particular architecture for optical interconnections is being studied at the University of Colorado at Boulder in the EMT/NANO project, called Broadcast Optical Interconnects for Global Communication in Many-Core Chip Multiprocessor. As with most types of networks, including optical networks, one of the most important components are modulators. Therefore adequate design and fabrication techniques for modulators contribute to higher modulation rates which lead to improve the efficiency and reductions in the latency of the optical network. Electro-optical modulators are presented in this study as an alternative to achieve this end. In recent years, nonlinear optical (NLO) materials have been used for the fabrication of high-speed electro-optical modulators. Polymers doped with chromophores are an alternative among NLO materials because they can develop large electro-optic coefficients and low dielectric constants. These two factors are critical for achieving high-speed modulation rates. These polymer-based electro-optical modulators can be fabricated using standard laboratory techniques, such as polymer spin-coating onto substrates, UV bleaching to achieve a refractive index variation and poling techniques to align the chromophores in cured polymers. The design of the electro-optic modulators require the use of the optical parameters of the materials to be used. Therefore the characterization of these materials is a required previous step. This characterization is performed by the fabrication of chromophores-doped polymer samples and conducting transmission and

High-speed packet switching network to link computers

CERN Document Server

Gerard, F M

1980-01-01

Virtually all of the experiments conducted at CERN use minicomputers today; some simply acquire data and store results on magnetic tape while others actually control experiments and help to process the resulting data. Currently there are more than two hundred minicomputers being used in the laboratory. In order to provide the minicomputer users with access to facilities available on mainframes and also to provide intercommunication between various experimental minicomputers, CERN opted for a packet switching network back in 1975. It was decided to use Modcomp II computers as switching nodes. The only software to be taken was a communications-oriented operating system called Maxcom. Today eight Modcomp II 16-bit computers plus six newer Classic minicomputers from Modular Computer Services have been purchased for the CERNET data communications networks. The current configuration comprises 11 nodes connecting more than 40 user machines to one another and to the laboratory's central computing facility. (0 refs).

Automated Transportation Management System (ATMS) user's manual. Revision 1

International Nuclear Information System (INIS)

Smith, P.D.

1994-01-01

The Automated Transportation Management System (ATMS) Software User Guide (SUG) constitutes the user procedures for the ATMS System. Information in this document will be used by the user to operate the automated system. It is intended to be used as a reference manual to guide and direct the user(s) through the ATMS software product and its environment. The objectives of ATMS are as follows: to better support the Procurement function with freight rate information; to free Transportation Logistics personnel from routine activities such as the auditing and input of freight billing information; to comply with Headquarters Department of Energy-Inspector General (DOE-IG) audit findings to automate transportation management functions; to reduce the keying of data into the Shipment Mobility Accountability Collection (SMAC) database; and to provide automation for the preparing of Bill of Lading, Declaration of Dangerous Goods, Emergency Response Guide and shipping Labels using HM181 Retrieval of hazardous material table text information

Marketing Career Speed Networking: A Classroom Event to Foster Career Awareness

Science.gov (United States)

Buff, Cheryl L.; O'Connor, Suzanne

2012-01-01

This paper describes the design, implementation, and evaluation of a marketing career speed networking event held during class time in two sections of the consumer behavior class. The event was coordinated through a partnering effort with marketing faculty and the college's Career Center. A total of 57 students participated in the event, providing…

ATM/RB1 mutations predict shorter overall survival in urothelial cancer.

Science.gov (United States)

Yin, Ming; Grivas, Petros; Emamekhoo, Hamid; Mendiratta, Prateek; Ali, Siraj; Hsu, JoAnn; Vasekar, Monali; Drabick, Joseph J; Pal, Sumanta; Joshi, Monika

2018-03-30

Mutations of DNA repair genes, e.g. ATM/RB1 , are frequently found in urothelial cancer (UC) and have been associated with better response to cisplatin-based chemotherapy. Further external validation of the prognostic value of ATM/RB1 mutations in UC can inform clinical decision making and trial designs. In the discovery dataset, ATM/RB1 mutations were present in 24% of patients and were associated with shorter OS (adjusted HR 2.67, 95% CI, 1.45-4.92, p = 0.002). There was a higher mutation load in patients carrying ATM/RB1 mutations (median mutation load: 6.7 versus 5.5 per Mb, p = 0.072). In the validation dataset, ATM/RB1 mutations were present in 22.2% of patients and were non-significantly associated with shorter OS (adjusted HR 1.87, 95% CI, 0.97-3.59, p = 0.06) and higher mutation load (median mutation load: 8.1 versus 7.2 per Mb, p = 0.126). Exome sequencing data of 130 bladder UC patients from The Cancer Genome Atlas (TCGA) dataset were analyzed as a discovery cohort to determine the prognostic value of ATM/RB1 mutations. Results were validated in an independent cohort of 81 advanced UC patients. Cox proportional hazard regression analysis was performed to calculate the hazard ratio (HR) and 95% confidence interval (CI) to compare overall survival (OS). ATM/RB1 mutations may be a biomarker of poor prognosis in unselected UC patients and may correlate with higher mutational load. Further studies are required to determine factors that can further stratify prognosis and evaluate predictive role of ATM/RB1 mutation status to immunotherapy and platinum-based chemotherapy.

A High-Speed Design of Montgomery Multiplier

Science.gov (United States)

Fan, Yibo; Ikenaga, Takeshi; Goto, Satoshi

With the increase of key length used in public cryptographic algorithms such as RSA and ECC, the speed of Montgomery multiplication becomes a bottleneck. This paper proposes a high speed design of Montgomery multiplier. Firstly, a modified scalable high-radix Montgomery algorithm is proposed to reduce critical path. Secondly, a high-radix clock-saving dataflow is proposed to support high-radix operation and one clock cycle delay in dataflow. Finally, a hardware-reused architecture is proposed to reduce the hardware cost and a parallel radix-16 design of data path is proposed to accelerate the speed. By using HHNEC 0.25μm standard cell library, the implementation results show that the total cost of Montgomery multiplier is 130 KGates, the clock frequency is 180MHz and the throughput of 1024-bit RSA encryption is 352kbps. This design is suitable to be used in high speed RSA or ECC encryption/decryption. As a scalable design, it supports any key-length encryption/decryption up to the size of on-chip memory.

Prefrontal, posterior parietal and sensorimotor network activity underlying speed control during walking

Directory of Open Access Journals (Sweden)

Thomas C Bulea

2015-05-01

Full Text Available Accumulating evidence suggests cortical circuits may contribute to control of human locomotion. Here, noninvasive electroencephalography (EEG recorded from able-bodied volunteers during a novel treadmill walking paradigm was used to assess neural correlates of walking. A systematic processing method, including a recently developed subspace reconstruction algorithm, reduced movement-related EEG artifact prior to independent component analysis and dipole source localization. We quantified cortical activity while participants tracked slow and fast target speeds across two treadmill conditions: an active mode that adjusted belt speed based on user movements and a passive mode reflecting a typical treadmill. Our results reveal frequency specific, multi-focal task related changes in cortical oscillations elicited by active walking. Low γ band power, localized to the prefrontal and posterior parietal cortices, was significantly increased during double support and early swing phases, critical points in the gait cycle since the active controller adjusted speed based on pelvis position and swing foot velocity. These phasic γ band synchronizations provide evidence that prefrontal and posterior parietal networks, previously implicated in visuo-spatial and somotosensory integration, are engaged to enhance lower limb control during gait. Sustained μ and β band desynchronization within sensorimotor cortex, a neural correlate for movement, was observed during walking thereby validating our methods for isolating cortical activity. Our results also demonstrate the utility of EEG recorded during locomotion for probing the multi-regional cortical networks which underpin its execution. For example, the cortical network engagement elicited by the active treadmill suggests that it may enhance neuroplasticity for more effective motor training.

High speed switching for computer and communication networks

NARCIS (Netherlands)

Dorren, H.J.S.

2014-01-01

The role of data centers and computers are vital for the future of our data-centric society. Historically the performance of data-centers is increasing with a factor 100-1000 every ten years and as a result of this the capacity of the data-center communication network has to scale accordingly. This

Morphology and genomic hallmarks of breast tumours developed by ATM deleterious variant carriers.

Science.gov (United States)

Renault, Anne-Laure; Mebirouk, Noura; Fuhrmann, Laetitia; Bataillon, Guillaume; Cavaciuti, Eve; Le Gal, Dorothée; Girard, Elodie; Popova, Tatiana; La Rosa, Philippe; Beauvallet, Juana; Eon-Marchais, Séverine; Dondon, Marie-Gabrielle; d'Enghien, Catherine Dubois; Laugé, Anthony; Chemlali, Walid; Raynal, Virginie; Labbé, Martine; Bièche, Ivan; Baulande, Sylvain; Bay, Jacques-Olivier; Berthet, Pascaline; Caron, Olivier; Buecher, Bruno; Faivre, Laurence; Fresnay, Marc; Gauthier-Villars, Marion; Gesta, Paul; Janin, Nicolas; Lejeune, Sophie; Maugard, Christine; Moutton, Sébastien; Venat-Bouvet, Laurence; Zattara, Hélène; Fricker, Jean-Pierre; Gladieff, Laurence; Coupier, Isabelle; Chenevix-Trench, Georgia; Hall, Janet; Vincent-Salomon, Anne; Stoppa-Lyonnet, Dominique; Andrieu, Nadine; Lesueur, Fabienne

2018-04-17

The ataxia telangiectasia mutated (ATM) gene is a moderate-risk breast cancer susceptibility gene; germline loss-of-function variants are found in up to 3% of hereditary breast and ovarian cancer (HBOC) families who undergo genetic testing. So far, no clear histopathological and molecular features of breast tumours occurring in ATM deleterious variant carriers have been described, but identification of an ATM-associated tumour signature may help in patient management. To characterise hallmarks of ATM-associated tumours, we performed systematic pathology review of tumours from 21 participants from ataxia-telangiectasia families and 18 participants from HBOC families, as well as copy number profiling on a subset of 23 tumours. Morphology of ATM-associated tumours was compared with that of 599 patients with no BRCA1 and BRCA2 mutations from a hospital-based series, as well as with data from The Cancer Genome Atlas. Absolute copy number and loss of heterozygosity (LOH) profiles were obtained from the OncoScan SNP array. In addition, we performed whole-genome sequencing on four tumours from ATM loss-of-function variant carriers with available frozen material. We found that ATM-associated tumours belong mostly to the luminal B subtype, are tetraploid and show LOH at the ATM locus at 11q22-23. Unlike tumours in which BRCA1 or BRCA2 is inactivated, tumours arising in ATM deleterious variant carriers are not associated with increased large-scale genomic instability as measured by the large-scale state transitions signature. Losses at 13q14.11-q14.3, 17p13.2-p12, 21p11.2-p11.1 and 22q11.23 were observed. Somatic alterations at these loci may therefore represent biomarkers for ATM testing and harbour driver mutations in potentially 'druggable' genes that would allow patients to be directed towards tailored therapeutic strategies. Although ATM is involved in the DNA damage response, ATM-associated tumours are distinct from BRCA1-associated tumours in terms of morphological

Modified Hermite Pulse-Based Wideband Communication for High-Speed Data Transfer in Wireless Sensor Applications

Directory of Open Access Journals (Sweden)

Kushal P. Pradhan

2017-12-01

Full Text Available With technological advances in the field of communication, the need for reliable high-speed data transfer is increasing. The deployment of large number of wireless sensors for remote monitoring and control and streaming of high definition video, voice and image data, etc. are imposing a challenge to the existing network bandwidth allocation for reliable communication. Two novel schemes for ultra-wide band (UWB communication technology have been proposed in this paper with the key objective of intensifying the data rate by taking advantage of the orthogonal properties of the modified Hermite pulse (MHP. In the first scheme, a composite pulse is transmitted and in the second scheme, a sequence of multi-order orthogonal pulses is transmitted in the place of a single UWB pulse. The MHP pulses exhibit a mutually orthogonal property between different ordered pulses and due to this property, simultaneous transmission is achieved without collision in the UWB system, resulting in an increase in transmission capacity or improved bit error rate. The proposed schemes for enhanced data rate will offer high volume data monitoring, assessment, and control of wireless devices without overburdening the network bandwidth and pave the way for new platforms for future high-speed wireless sensor applications.

Potential scenarios of concern for high speed rail operations

Science.gov (United States)

2011-03-16

Currently, multiple operating authorities are proposing the : introduction of high-speed rail service in the United States. : While high-speed rail service shares a number of basic : principles with conventional-speed rail service, the operational : ...

ATM Protein Physically and Functionally Interacts with Proliferating Cell Nuclear Antigen to Regulate DNA Synthesis*

Science.gov (United States)

Gamper, Armin M.; Choi, Serah; Matsumoto, Yoshihiro; Banerjee, Dibyendu; Tomkinson, Alan E.; Bakkenist, Christopher J.

2012-01-01

Ataxia telangiectasia (A-T) is a pleiotropic disease, with a characteristic hypersensitivity to ionizing radiation that is caused by biallelic mutations in A-T mutated (ATM), a gene encoding a protein kinase critical for the induction of cellular responses to DNA damage, particularly to DNA double strand breaks. A long known characteristic of A-T cells is their ability to synthesize DNA even in the presence of ionizing radiation-induced DNA damage, a phenomenon termed radioresistant DNA synthesis. We previously reported that ATM kinase inhibition, but not ATM protein disruption, blocks sister chromatid exchange following DNA damage. We now show that ATM kinase inhibition, but not ATM protein disruption, also inhibits DNA synthesis. Investigating a potential physical interaction of ATM with the DNA replication machinery, we found that ATM co-precipitates with proliferating cell nuclear antigen (PCNA) from cellular extracts. Using bacterially purified ATM truncation mutants and in vitro translated PCNA, we showed that the interaction is direct and mediated by the C terminus of ATM. Indeed, a 20-amino acid region close to the kinase domain is sufficient for strong binding to PCNA. This binding is specific to ATM, because the homologous regions of other PIKK members, including the closely related kinase A-T and Rad3-related (ATR), did not bind PCNA. ATM was found to bind two regions in PCNA. To examine the functional significance of the interaction between ATM and PCNA, we tested the ability of ATM to stimulate DNA synthesis by DNA polymerase δ, which is implicated in both DNA replication and DNA repair processes. ATM was observed to stimulate DNA polymerase activity in a PCNA-dependent manner. PMID:22362778

Hour-Glass Neural Network Based Daily Money Flow Estimation for Automatic Teller Machines

Science.gov (United States)

Karungaru, Stephen; Akashi, Takuya; Nakano, Miyoko; Fukumi, Minoru

Monetary transactions using Automated Teller Machines (ATMs) have become a normal part of our daily lives. At ATMs, one can withdraw, send or debit money and even update passbooks among many other possible functions. ATMs are turning the banking sector into a ubiquitous service. However, while the advantages for the ATM users (financial institution customers) are many, the financial institution side faces an uphill task in management and maintaining the cash flow in the ATMs. On one hand, too much money in a rarely used ATM is wasteful, while on the other, insufficient amounts would adversely affect the customers and may result in a lost business opportunity for the financial institution. Therefore, in this paper, we propose a daily cash flow estimation system using neural networks that enables better daily forecasting of the money required at the ATMs. The neural network used in this work is a five layered hour glass shaped structure that achieves fast learning, even for the time series data for which seasonality and trend feature extraction is difficult. Feature extraction is carried out using the Akamatsu Integral and Differential transforms. This work achieves an average estimation accuracy of 92.6%.

Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress

DEFF Research Database (Denmark)

Munk, Stephanie; Sigurðsson, Jón Otti; Xiao, Zhenyu

2017-01-01

The mechanisms that protect eukaryotic DNA during the cumbersome task of replication depend on the precise coordination of several post-translational modification (PTM)-based signaling networks. Phosphorylation is a well-known regulator of the replication stress response, and recently an essentia....... They analyze changes in the SUMO and phosphoproteome after MMC and hydroxyurea treatments and find that the DNA damage response kinases ATR and ATM globally regulate SUMOylation upon replication stress and fork breakage....

High-Speed Rail for Central and Eastern European Countries: A Conference Report

Directory of Open Access Journals (Sweden)

Jandová Monika

2016-09-01

Full Text Available The European transport strategy promotes the role of railways and expects that the key role in passenger transport should be played by high-speed rail (HSR. Although the core network of high-speed lines has already been built and is operating in Western Europe, there has been little coverage so far in Central and Eastern Europe (CEE. The aim of the conference “High-Speed Rail for CEE Countries” that took place in Prague in June 2016 was to put together academics, policy-makers, and practitioners interested in HSR and to formulate recommendations for CEE countries based on West European countries’ experience. Based on the conference presentations and subsequent discussion, the following conclusions were formulated. Firstly, there are many crucial differences in national HSR build-up and operation, which means that former experience of Western Europe is not directly applicable to CEE countries. Secondly, in comparing presentations discussing experiences in France, Britain, Italy, and Germany, it was concluded that the German approach-upgrading existing lines where possible and only building new lines for bottleneck sections-was the most likely appropriate solution in CEE. Lastly, CEE has the additional problem of many border crossings, with a reduction of traffic in comparison with purely domestic routes, and this effect has to be taken into account.

Preslikavanje parametara kvaliteta usluga na protokole ATM mreža

OpenAIRE

Milojko Jevtović

2005-01-01

Preslikavanje parametara kvaliteta usluga (Quality of Service - QoS) jedan je od bitnih elemenata u koncepciji ATM (Asynchronous Transfer Mode) širokopojasnih mreža. U radu su opisani parametri QoS-a (verovatnoće pogrešnih ramova i ćelija, propusni opseg, kašnjenje ćelija, varijacija kašnjenja) koji se preslikavaju na protokole ATM mreža. Preslikavanje se izvodi između korisničkog i aplikacionog QoS-a, a aplikacioni QoS se preslikava na QoS prenosa i komutacije, odnosno na ATM protokole, tzv....

High-Speed Non-Volatile Optical Memory: Achievements and Challenges

Directory of Open Access Journals (Sweden)

Vadym Zayets

2017-01-01

Full Text Available We have proposed, fabricated, and studied a new design of a high-speed optical non-volatile memory. The recoding mechanism of the proposed memory utilizes a magnetization reversal of a nanomagnet by a spin-polarized photocurrent. It was shown experimentally that the operational speed of this memory may be extremely fast above 1 TBit/s. The challenges to realize both a high-speed recording and a high-speed reading are discussed. The memory is compact, integratable, and compatible with present semiconductor technology. If realized, it will advance data processing and computing technology towards a faster operation speed.

Function of the ATR N-terminal domain revealed by an ATM/ATR chimera

International Nuclear Information System (INIS)

Chen Xinping; Zhao Runxiang; Glick, Gloria G.; Cortez, David

2007-01-01

The ATM and ATR kinases function at the apex of checkpoint signaling pathways. These kinases share significant sequence similarity, phosphorylate many of the same substrates, and have overlapping roles in initiating cell cycle checkpoints. However, they sense DNA damage through distinct mechanisms. ATR primarily senses single stranded DNA (ssDNA) through its interaction with ATRIP, and ATM senses double strand breaks through its interaction with Nbs1. We determined that the N-terminus of ATR contains a domain that binds ATRIP. Attaching this domain to ATM allowed the fusion protein (ATM*) to bind ATRIP and associate with RPA-coated ssDNA. ATM* also gained the ability to localize efficiently to stalled replication forks as well as double strand breaks. Despite having normal kinase activity when tested in vitro and being phosphorylated on S1981 in vivo, ATM* is defective in checkpoint signaling and does not complement cellular deficiencies in either ATM or ATR. These data indicate that the N-terminus of ATR is sufficient to bind ATRIP and to promote localization to sites of replication stress

High speed laser tomography system

Science.gov (United States)

Samsonov, D.; Elsaesser, A.; Edwards, A.; Thomas, H. M.; Morfill, G. E.

2008-03-01

A high speed laser tomography system was developed capable of acquiring three-dimensional (3D) images of optically thin clouds of moving micron-sized particles. It operates by parallel-shifting an illuminating laser sheet with a pair of galvanometer-driven mirrors and synchronously recording two-dimensional (2D) images of thin slices of the imaged volume. The maximum scanning speed achieved was 120000slices/s, sequences of 24 volume scans (up to 256 slices each) have been obtained. The 2D slices were stacked to form 3D images of the volume, then the positions of the particles were identified and followed in the consecutive scans. The system was used to image a complex plasma with particles moving at speeds up to cm/s.

On the Experimental and Theoretical Investigations of Lean Partially Premixed Combustion, Burning Speed, Flame Instability and Plasma Formation of Alternative Fuels at High Temperatures and Pressures

Science.gov (United States)

Askari, Omid

composition and thermodynamic properties. The method was applied to compute the thermodynamic properties of hydrogen/air and methane/air plasma mixtures for a wide range of temperatures (1,000-100,000 K), pressures (10-6-100 atm) and different equivalence ratios within flammability limit. In calculating the individual thermodynamic properties of the atomic species, the Debye-Huckel cutoff criterion has been used for terminating the series expression of the electronic partition function. A new differential-based multi-shell model was developed in conjunction with Schlieren photography to measure laminar burning speed and to study the flame instabilities for different alternative fuels such as syngas and GTL. Flame instabilities such as cracking and wrinkling were observed during flame propagation and discussed in terms of the hydrodynamic and thermo-diffusive effects. Laminar burning speeds were measured using pressure rise data during flame propagation and power law correlations were developed over a wide range of temperatures, pressures and equivalence ratios. As a part of this work, the effect of EGR addition and substitution of nitrogen with helium in air on flame morphology and laminar burning speed were extensively investigated. The effect of cell formation on flame surface area of syngas fuel in terms of a newly defined parameter called cellularity factor was also evaluated. In addition to that the experimental onset of auto-ignition and theoretical ignition delay times of premixed GTL/air mixture were determined at high pressures and low temperatures over a wide range of equivalence ratios.

A Historical Review of High Speed Metal Forming

OpenAIRE

Zittel, G.

2010-01-01

This paper will present a Historical Review of High Speed Metal Forming beginning with the first thought of forming metal by using an electromagnetic impulse to today, whereby High Speed Metal Forming is an accepted production process. Although this paper will briefly cover the basic physics of the process, it will not dwell on it. It will rather show how the industrial acceptance of High Speed Metal Forming is tightly connected to the knowledge acquired from many applications studies. These ...

Redes Atm de Alto Desempenho

Directory of Open Access Journals (Sweden)

Flávia Oliveira Santos de Sá Lisboa

2015-12-01

Full Text Available A tendência atual de integração de serviços de dados, voz e vídeo, estimulada pelo pleno sucesso da Internet, aumentou a demanda por maior banda e melhor desempenho nas redes de comunicação de dados. Neste contexto, a tecnologia ATM (Asynchronous Transfer Mode vem sendo utilizada na implementação de backbone de LANs e WANs, justamente por oferecer a possibilidade de integração de serviços com qualidade, alta escalabilidade e altas taxas de transferência em banda larga. Neste artigo serão abordados os principais conceitos relacionados à tecnologia ATM, suas vantagens e desvantagens em face de outras tecnologias (como Fast e Gigabit Ethernet, além de casos de sua utilização em empresas e instituições de ensino.

High-speed photography. Technique and evolution

International Nuclear Information System (INIS)

Sanchez-Tembleque, R.

1981-01-01

It is intended to present some general considerations about ''Higg-speed photography'' as a tool of work common in mos research laboratories in the world. ''High-speed photography'' relies on the principles of photography of actions, that change rapidly with the time. The evolution of this technique goes along with the discovering of new phenomena in wich higher speeds are involved. At present is normal to deal with changing rates involving picoseconds times (10 -12 s) and new developments on the field of femtosecond (10 -15 s) theoretically are contemplated. (author)

4th ENRI International Workshop on ATM/CNS

CERN Document Server

2017-01-01

This book is a compilation of selected papers from the 4th ENRI International Workshop on ATM/CNS (EIWAC2015). The work focuses on novel techniques for aviation infrastructure in air traffic management (ATM) and communications, navigation, surveillance, and informatics (CNSI) domains. The contents make valuable contributions to academic researchers, engineers in the industry, and regulators of aviation authorities. As well, readers will encounter new ideas for realizing a more efficient and safer aviation system. .

Review of High-Speed Fiber Optic Grating Sensors Systems

Energy Technology Data Exchange (ETDEWEB)

Udd, E; Benterou, J; May, C; Mihailov, S J; Lu, P

2010-03-24

Fiber grating sensors can be used to support a wide variety of high speed measurement applications. This includes measurements of vibrations on bridges, traffic monitoring on freeways, ultrasonic detection to support non-destructive tests on metal plates and providing details of detonation events. This paper provides a brief overview of some of the techniques that have been used to support high speed measurements using fiber grating sensors over frequency ranges from 10s of kHz, to MHZ and finally toward frequencies approaching the GHz regime. Very early in the development of fiber grating sensor systems it was realized that a high speed fiber grating sensor system could be realized by placing an optical filter that might be a fiber grating in front of a detector so that spectral changes in the reflection from a fiber grating were amplitude modulated. In principal the only limitation on this type of system involved the speed of the output detector which with the development of high speed communication links moved from the regime of 10s of MHz toward 10s of GHz. The earliest deployed systems involved civil structures including measurements of the strain fields on composite utility poles and missile bodies during break tests, bridges and freeways. This was followed by a series of developments that included high speed fiber grating sensors to support nondestructive testing via ultrasonic wave detection, high speed machining and monitoring ship hulls. Each of these applications involved monitoring mechanical motion of structures and thus interest was in speeds up to a few 10s of MHz. Most recently there has been interest in using fiber grating to monitor the very high speed events such as detonations and this has led to utilization of fiber gratings that are consumed during an event that may require detection speeds of hundreds of MHz and in the future multiple GHz.

High-Speed Videography Instrumentation And Procedures

Science.gov (United States)

Miller, C. E.

1982-02-01

High-speed videography has been an electronic analog of low-speed film cameras, but having the advantages of instant-replay and simplicity of operation. Recent advances have pushed frame-rates into the realm of the rotating prism camera. Some characteristics of videography systems are discussed in conjunction with applications in sports analysis, and with sports equipment testing.

ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress.

Science.gov (United States)

Liu, Rui; King, Ashleigh; Hoch, Nicolas C; Chang, Catherine; Kelly, Gemma L; Deans, Andrew J; Heierhorst, Jörg

2017-09-01

The ATM kinase plays critical roles in the response to DNA double-strand breaks, and can also be activated by prolonged DNA replication blocks. It has recently been proposed that replication stress-dependent ATM activation is mediated by ASCIZ (also known as ATMIN, ZNF822), an essential developmental transcription factor. In contrast, we show here that ATM activation, and phosphorylation of its substrates KAP1, p53 and H2AX in response to the replication blocking agent aphidicolin was unaffected in both immortalized and primary ASCIZ/ATMIN-deficient murine embryonic fibroblasts compared to control cells. Similar results were also obtained in human ASCIZ/ATMIN-deleted lymphoma cells. The results demonstrate that ASCIZ/ATMIN is dispensable for ATM activation, and contradict the previously reported dependence of ATM on ASCIZ/ATMIN. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Role of ataxia-telangiectasia mutated (ATM) in porcine oocyte in vitro maturation.

Science.gov (United States)

Lin, Zi-Li; Kim, Nam-Hyung

2015-06-01

Ataxia-telangiectasia mutated (ATM) is critical for the DNA damage response, cell cycle checkpoints, and apoptosis. Significant effort has focused on elucidating the relationship between ATM and other nuclear signal transducers; however, little is known about the connection between ATM and oocyte meiotic maturation. We investigated the function of ATM in porcine oocytes. ATM was expressed at all stages of oocyte maturation and localized predominantly in the nucleus. Furthermore, the ATM-specific inhibitor KU-55933 blocked porcine oocyte maturation, reducing the percentages of oocytes that underwent germinal vesicle breakdown (GVBD) and first polar body extrusion. KU-55933 also decreased the expression of DNA damage-related genes (breast cancer 1, budding uninhibited by benzimidazoles 1, and P53) and reduced the mRNA and protein levels of AKT and other cell cycle-regulated genes that are predominantly expressed during G2/M phase, including bone morphogenetic protein 15, growth differentiation factor 9, cell division cycle protein 2, cyclinB1, and AKT. KU-55933 treatment decreased the developmental potential of blastocysts following parthenogenetic activation and increased the level of apoptosis. Together, these data suggested that ATM influenced the meiotic and cytoplasmic maturation of porcine oocytes, potentially by decreasing their sensitivity to DNA strand breaks, stimulating the AKT pathway, and/or altering the expression of other maternal genes. © 2015 International Federation for Cell Biology.

Silicon Monoxide at 1 atm and Elevated Pressures: Crystalline or Amorphous?

KAUST Repository

AlKaabi, Khalid

2014-03-05

The absence of a crystalline SiO phase under ordinary conditions is an anomaly in the sequence of group 14 monoxides. We explore theoretically ordered ground-state and amorphous structures for SiO at P = 1 atm, and crystalline phases also at pressures up to 200 GPa. Several competitive ground-state P = 1 atm structures are found, perforce with Si-Si bonds, and possessing Si-O-Si bridges similar to those in silica (SiO2) polymorphs. The most stable of these static structures is enthalpically just a little more stable than a calculated random bond model of amorphous SiO. In that model we find no segregation into regions of amorphous Si and amorphous SiO2. The P = 1 atm structures are all semiconducting. As the pressure is increased, intriguing new crystalline structures evolve, incorporating Si triangular nets or strips and stishovite-like regions. A heat of formation of crystalline SiO is computed; it is found to be the most negative of all the group 14 monoxides. Yet, given the stability of SiO2, the disproportionation 2SiO (s) → Si(s)+SiO2(s) is exothermic, falling right into the series of group 14 monoxides, and ranging from a highly negative ΔH of disproportionation for CO to highly positive for PbO. There is no major change in the heat of disproportionation with pressure, i.e., no range of stability of SiO with respect to SiO2. The high-pressure SiO phases are metallic. © 2014 American Chemical Society.

ATMS Step By Step.

Science.gov (United States)

National Library of Australia, Canberra.

This manual is designed to provide an introduction and basic guide to the use of IBM's Advanced Text Management System (ATMS), the text processing system to be used for the creation of Australian data bases within AUSINET. Instructions are provided for using the system to enter, store, retrieve, and modify data, which may then be displayed at the…

High-speed and intercity passenger rail testing strategy.

Science.gov (United States)

2013-05-01

This high-speed and intercity passenger rail (HSIPR) testing strategy addresses the requirements for testing of high-speed train sets and technology before introduction to the North American railroad system. The report documents the results of a surv...

Application of high-speed photography to hydrodynamic instability research

International Nuclear Information System (INIS)

Chang Lihua; Li Zuoyou; Xiao Zhengfei; Zou Liyong; Liu Jinhong; Xiong Xueshi

2012-01-01

High-speed photography is used to study the Rayleigh-Taylor instability of air-water interface driven by high- pressure exploding gas. Clear images illustrating the instability are obtained, along with the air bubble peak speed and turbulent mixing speed. The RM (Richtmyer-Meshkov) instability of air/SF 6 interface driven by shock wave is also researched by using high-speed Schlieren technique on the horizontal shock tube and primary experimental results are obtained, which show the change of the turbulent mixing region clearly. (authors)

Development of high-speed video cameras

Science.gov (United States)

Etoh, Takeharu G.; Takehara, Kohsei; Okinaka, Tomoo; Takano, Yasuhide; Ruckelshausen, Arno; Poggemann, Dirk

2001-04-01

Presented in this paper is an outline of the R and D activities on high-speed video cameras, which have been done in Kinki University since more than ten years ago, and are currently proceeded as an international cooperative project with University of Applied Sciences Osnabruck and other organizations. Extensive marketing researches have been done, (1) on user's requirements on high-speed multi-framing and video cameras by questionnaires and hearings, and (2) on current availability of the cameras of this sort by search of journals and websites. Both of them support necessity of development of a high-speed video camera of more than 1 million fps. A video camera of 4,500 fps with parallel readout was developed in 1991. A video camera with triple sensors was developed in 1996. The sensor is the same one as developed for the previous camera. The frame rate is 50 million fps for triple-framing and 4,500 fps for triple-light-wave framing, including color image capturing. Idea on a video camera of 1 million fps with an ISIS, In-situ Storage Image Sensor, was proposed in 1993 at first, and has been continuously improved. A test sensor was developed in early 2000, and successfully captured images at 62,500 fps. Currently, design of a prototype ISIS is going on, and, hopefully, will be fabricated in near future. Epoch-making cameras in history of development of high-speed video cameras by other persons are also briefly reviewed.

Risk of cancer by ATM missense mutations in the general population

DEFF Research Database (Denmark)

Dombernowsky, Sarah Louise; Weischer, Maren; Allin, Kristine Højgaard

2008-01-01

PURPOSE: Truncating and missense mutations in the ATM gene, which cause insufficient DNA damage surveillance, allow damaged cells to proceed into mitosis, which eventually results in increased cancer susceptibility. We tested the hypotheses that ATM Ser49Cys and ATM Ser707Pro heterozygosity......: Multifactorially adjusted hazard ratios for ATM Ser49Cys heterozygotes versus noncarriers were 1.2 (95% CI, 0.9 to 1.5) for cancer overall, 0.8 (95% CI, 0.3 to 2.0) for breast cancer, 4.8 (95% CI, 2.2 to 11) for melanoma, 2.3 (95% CI, 1.1 to 5.0) for prostate cancer, and 3.4 (95% CI, 1.1 to 11) for cancer...... of the oral cavity/pharynx. Multifactorially adjusted hazard ratios for ATM Ser707Pro heterozygotes versus noncarriers were 0.8 (95% CI, 0.6 to 1.2) for cancer overall, 0.6 (95% CI, 0.2 to 1.6) for breast cancer, 10 (95% CI, 1.1 to 93) for thyroid/other endocrine tumors, and 2.7 (95% CI, 1.0 to 7...

High-speed imaging of inhomogeneous ignition in a shock tube

Science.gov (United States)

Tulgestke, A. M.; Johnson, S. E.; Davidson, D. F.; Hanson, R. K.

2018-05-01

Homogeneous and inhomogeneous ignition of real and surrogate fuels were imaged in two Stanford shock tubes, revealing the influence of small particle fragmentation. n-Heptane, iso-octane, and Jet A were studied, each mixed in an oxidizer containing 21% oxygen and ignited at low temperatures (900-1000 K), low pressures (1-2 atm), with an equivalence ratio of 0.5. Visible images (350-1050 nm) were captured through the shock tube endwall using a high-speed camera. Particles were found to arrive near the endwalls of the shock tubes approximately 5 ms after reflection of the incident shock wave. Reflected shock wave experiments using diaphragm materials of Lexan and steel were investigated. Particles collected from the shock tubes after each experiment were found to match the material of the diaphragm burst during the experiment. Following each experiment, the shock tubes were cleaned by scrubbing with cotton cloths soaked with acetone. Particles were observed to fragment after arrival near the endwall, often leading to inhomogeneous ignition of the fuel. Distinctly more particles were observed during experiments using steel diaphragms. In experiments exhibiting inhomogeneous ignition, flames were observed to grow radially until all the fuel within the cross section of the shock tube had been consumed. The influence of diluent gas (argon or helium) was also investigated. The use of He diluent gas was found to suppress the number of particles capable of causing inhomogeneous flames. The use of He thus allowed time history studies of ignition to extend past the test times that would have been limited by inhomogeneous ignition.

COMMUNICA TION NETWORK FOR TELEMEDICINE Debretsion G ...

African Journals Online (AJOL)

(3) continuing medical education and (4) Training the medical ... Telemedicine system consists of at least five major subsystems. [l,4]. ... and wire-line (optical network) telecommunication facilities .... An ATM network needs certain traffic control.

High-speed AFM of human chromosomes in liquid

Energy Technology Data Exchange (ETDEWEB)

Picco, L M; Dunton, P G; Ulcinas, A; Engledew, D J; Miles, M J [H H Wills Physics Laboratory and IRC in Nanotechnology, University of Bristol, Tyndall Avenue, Bristol BS8 1TL (United Kingdom); Hoshi, O; Ushiki, T [Division of Microscopic Anatomy and Bio-Imaging, Department of Cellular Function, Niigata University Graduate School of Medical and Dental Sciences, Asahimachi-Dori 1, Niigata, 951-8150 (Japan)], E-mail: m.j.miles@bristol.ac.uk

2008-09-24

Further developments of the previously reported high-speed contact-mode AFM are described. The technique is applied to the imaging of human chromosomes at video rate both in air and in water. These are the largest structures to have been imaged with high-speed AFM and the first imaging in liquid to be reported. A possible mechanism that allows such high-speed contact-mode imaging without significant damage to the sample is discussed in the context of the velocity dependence of the measured lateral force on the AFM tip.

Characterizing speed-independence of high-level designs

DEFF Research Database (Denmark)

Kishinevsky, Michael; Staunstrup, Jørgen

1994-01-01

This paper characterizes the speed-independence of high-level designs. The characterization is a condition on the design description ensuring that the behavior of the design is independent of the speeds of its components. The behavior of a circuit is modeled as a transition system, that allows data...... types, and internal as well as external non-determinism. This makes it possible to verify the speed-independence of a design without providing an explicit realization of the environment. The verification can be done mechanically. A number of experimental designs have been verified including a speed-independent...

Development of ultra high speed photographic system using high repetition rate visible laser

International Nuclear Information System (INIS)