WorldWideScience

Sample records for high availability ha

  1. High Availability Electronics Standards

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, R.S.; /SLAC

    2006-12-13

    Availability modeling of the proposed International Linear Collider (ILC) predicts unacceptably low uptime with current electronics systems designs. High Availability (HA) analysis is being used as a guideline for all major machine systems including sources, utilities, cryogenics, magnets, power supplies, instrumentation and controls. R&D teams are seeking to achieve total machine high availability with nominal impact on system cost. The focus of this paper is the investigation of commercial standard HA architectures and packaging for Accelerator Controls and Instrumentation. Application of HA design principles to power systems and detector instrumentation are also discussed.

  2. Proxmox high availability

    CERN Document Server

    Cheng, Simon MC

    2014-01-01

    If you want to know the secrets of virtualization and how to implement high availability on your services, this is the book for you. For those of you who are already using Proxmox, this book offers you the chance to build a high availability cluster with a distributed filesystem to further protect your system from failure.

  3. High availability IT services

    CERN Document Server

    Critchley, Terry

    2014-01-01

    This book starts with the basic premise that a service is comprised of the 3Ps-products, processes, and people. Moreover, these entities and their sub-entities interlink to support the services that end users require to run and support a business. This widens the scope of any availability design far beyond hardware and software. It also increases the potential for service failure for reasons beyond just hardware and software; the concept of logical outages. High Availability IT Services details the considerations for designing and running highly available ""services"" and not just the systems

  4. JUNOS High Availability

    CERN Document Server

    Sonderegger, James; Milne, Kieran; Palislamovic, Senad

    2009-01-01

    Whether your network is a complex carrier or just a few machines supporting a small enterprise, JUNOS High Availability will help you build reliable and resilient networks that include Juniper Networks devices. With this book's valuable advice on software upgrades, scalability, remote network monitoring and management, high-availability protocols such as VRRP, and more, you'll have your network uptime at the five, six, or even seven nines -- or 99.99999% of the time. Rather than focus on "greenfield" designs, the authors explain how to intelligently modify multi-vendor networks. You'll learn

  5. High availability using virtualization

    CERN Document Server

    Calzolari, Federico

    2009-01-01

    High availability has always been one of the main problems for a data center. Till now high availability was achieved by host per host redundancy, a highly expensive method in terms of hardware and human costs. A new approach to the problem can be offered by virtualization. Using virtualization, it is possible to achieve a redundancy system for all the services running on a data center. This new approach to high availability allows to share the running virtual machines over the servers up and running, by exploiting the features of the virtualization layer: start, stop and move virtual machines between physical hosts. The system (3RC) is based on a finite state machine with hysteresis, providing the possibility to restart each virtual machine over any physical host, or reinstall it from scratch. A complete infrastructure has been developed to install operating system and middleware in a few minutes. To virtualize the main servers of a data center, a new procedure has been developed to migrate physical to virtu...

  6. MongoDB high availability

    CERN Document Server

    Mehrabani, Afshin

    2014-01-01

    This book has a perfect balance of concepts and their practical implementation along with solutions to make a highly available MongoDB server with clear instructions and guidance. If you are using MongoDB in a production environment and need a solution to make a highly available MongoDB server, this book is ideal for you. Familiarity with MongoDB is expected so that you understand the content of this book.

  7. Introducing high availability to non high available designed applications

    Energy Technology Data Exchange (ETDEWEB)

    Zelnicek, Pierre; Kebschull, Udo [Kirchhoff Institute of Physics, Ruprecht-Karls-University Heidelberg (Germany); Haaland, Oystein Senneset [Physic Institut, University of Bergen, Bergen (Norway); Lindenstruth, Volker [Frankfurt Institut fuer Advanced Studies, University Frankfurt (Germany)

    2010-07-01

    A common problem in scientific computing environments and compute clusters today, is how to apply high availability to legacy applications. These applications are becoming more and more a problem in increasingly complex environments and with business grade availability constraints that requires 24 x 7 x 365 hours of operation. For a majority of applications, redesign is not an option. Either because of being closed source or the effort involved would be just as great as re-writing the application from scratch. Neither is letting normal operators restart and reconfigure the applications on backup nodes a solution. In addition to the possibility of mistakes from non-experts and the cost of keeping personnel at work 24/7, these kind of operations would require administrator privileges within the compute environment and would therefore be a security risk. Therefore, these legacy applications have to be monitored and if a failure occurs autonomously migrated to a working node. The pacemaker framework is designed for both tasks and ensures the availability of the legacy applications. Distributed redundant block devices are used for fault tolerant distributed data storage. The result is an Availability Environment Classification 2 (AEC-2).

  8. Cytoprotective responses in HaCaT keratinocytes exposed to high doses of curcumin.

    Science.gov (United States)

    Lundvig, Ditte M S; Pennings, Sebastiaan W C; Brouwer, Katrien M; Mtaya-Mlangwa, Matilda; Mugonzibwa, Emeria; Kuijpers-Jagtman, Anne Marie; Wagener, Frank A D T G; Von den Hoff, Johannes W

    2015-08-15

    Wound healing is a complex process that involves the well-coordinated interactions of different cell types. Topical application of high doses of curcumin, a plant-derived polyphenol, enhances both normal and diabetic cutaneous wound healing in rodents. For optimal tissue repair interactions between epidermal keratinocytes and dermal fibroblasts are essential. We previously demonstrated that curcumin increased reactive oxygen species (ROS) formation and apoptosis in dermal fibroblasts, which could be prevented by pre-induction of the cytoprotective enzyme heme oxygenase (HO)-1. To better understand the effects of curcumin on wound repair, we now assessed the effects of high doses of curcumin on the survival of HaCaT keratinocytes and the role of the HO system. We exposed HaCaT keratinocytes to curcumin in the presence or absence of the HO-1 inducers heme (FePP) and cobalt protoporphyrin (CoPP). We then assessed cell survival, ROS formation, and caspase activation. Curcumin induced caspase-dependent apoptosis in HaCaT keratinocytes via a ROS-dependent mechanism. Both FePP and CoPP induced HO-1 expression, but only FePP protected against curcumin-induced ROS formation and caspase-mediated apoptosis. In the presence of curcumin, FePP but not CoPP induced the expression of the iron scavenger ferritin. Together, our data show that the induction of ferritin, but not HO, protects HaCaT keratinocytes against cytotoxic doses of curcumin. The differential response of fibroblasts and keratinocytes to high curcumin doses may provide the basis for improving curcumin-based wound healing therapies. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. High-resolution crystal structure of HA33 of botulinum neurotoxin type B progenitor toxin complex

    Science.gov (United States)

    Lee, Kwangkook; Lam, Kwok-Ho; Kruel, Anna Magdalena; Perry, Kay; Rummel, Andreas; Jin, Rongsheng

    2014-01-01

    Botulinum neurotoxins (BoNTs) are produced as progenitor toxin complexes (PTCs) by Clostridium botulinum. The PTCs are composed of BoNT and non-toxic neurotoxin-associated proteins (NAPs), which serve to protect and deliver BoNT through the gastrointestinal tract in food borne botulism. HA33 is a key NAP component that specifically recognizes host carbohydrates and helps enrich PTC on the intestinal lumen preceding its transport across the epithelial barriers. Here, we report the crystal structure of HA33 of type B PTC (HA33/B) in complex with lactose at 1.46 Å resolution. The structural comparisons among HA33 of serotypes A–D reveal two different HA33–glycan interaction modes. The glycan-binding pockets on HA33/A and B are more suitable to recognize galactose-containing glycans in comparison to the equivalent sites on HA33/C and D. On the contrary, HA33/C and D could potentially recognize Neu5Ac as an independent receptor, whereas HA33/A and B do not. These findings indicate that the different oral toxicity and host susceptibility observed among different BoNT serotypes could be partly determined by the serotype-specific interaction between HA33 and host carbohydrate receptors. Furthermore, we have identified a key structural water molecule that mediates the HA33/B–lactose interactions. It provides the structural basis for development of new receptor-mimicking compounds, which have enhanced binding affinity with HA33 through their water-displacing moiety. PMID:24631690

  10. Multivalent HA DNA vaccination protects against highly pathogenic H5N1 avian influenza infection in chickens and mice.

    Directory of Open Access Journals (Sweden)

    Srinivas Rao

    Full Text Available BACKGROUND: Sustained outbreaks of highly pathogenic avian influenza (HPAI H5N1 in avian species increase the risk of reassortment and adaptation to humans. The ability to contain its spread in chickens would reduce this threat and help maintain the capacity for egg-based vaccine production. While vaccines offer the potential to control avian disease, a major concern of current vaccines is their potency and inability to protect against evolving avian influenza viruses. METHODOLOGY / PRINCIPAL FINDINGS: The ability of DNA vaccines encoding hemagglutinin (HA proteins from different HPAI H5N1 serotypes was evaluated for its ability to elicit neutralizing antibodies and to protect against homologous and heterologous HPAI H5N1 strain challenge in mice and chickens after DNA immunization by needle and syringe or with a pressure injection device. These vaccines elicited antibodies that neutralized multiple strains of HPAI H5N1 when given in combinations containing up to 10 HAs. The response was dose-dependent, and breadth was determined by the choice of the influenza virus HA in the vaccine. Monovalent and trivalent HA vaccines were tested first in mice and conferred protection against lethal H5N1 A/Vietnam/1203/2004 challenge 68 weeks after vaccination. In chickens, protection was observed against heterologous strains of HPAI H5N1 after vaccination with a trivalent H5 serotype DNA vaccine with doses as low as 5 microg DNA given twice either by intramuscular needle injection or with a needle-free device. CONCLUSIONS/SIGNIFICANCE: DNA vaccines offer a generic approach to influenza virus immunization applicable to multiple animal species. In addition, the ability to substitute plasmids encoding different strains enables rapid adaptation of the vaccine to newly evolving field isolates.

  11. High availability of hybrid wireless networks

    Science.gov (United States)

    Leitgeb, Erich; Gebhart, Michael; Birnbacher, Ulla; Kogler, Wolfgang; Schrotter, Peter

    2004-09-01

    Free Space Optical (FSO) links offer high bandwidth and the flexibility of wireless communication links. However, the availability of FSO links is limited by weather patterns like fog and heavy snowfall. Microwave based communication links operating at high frequencies (40 - 43 GHz) have similar characteristics like high data rates and needed line-of-sight. Link availability for microwave systems is limited by heavy rain. Combining FSO links with microwave links within a hybrid FSO/microwave communication network has the advantage of added redundancy and higher link availability. Measurements over a period of one year show a combined availability of 99.93% for the climatic region of Graz, Austria) which proves that the combination of both technologies leads to a highly available wireless connection offering high bandwidth.

  12. High plant availability of phosphorus and low availability of cadmium in four biomass combustion ashes

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xiaoxi, E-mail: Xiaoxi.Li@agro.au.dk; Rubæk, Gitte H.; Sørensen, Peter

    2016-07-01

    For biomass combustion to become a sustainable energy production system, it is crucial to minimise landfill of biomass ashes, to recycle the nutrients and to minimise the undesirable impact of hazardous substances in the ash. In order to test the plant availability of phosphorus (P) and cadmium (Cd) in four biomass ashes, we conducted two pot experiments on a P-depleted soil and one mini-plot field experiment on a soil with adequate P status. Test plants were spring barley and Italian ryegrass. Ash applications were compared to triple superphosphate (TSP) and a control without P application. Both TSP and ash significantly increased crop yields and P uptake on the P-depleted soil. In contrast, on the adequate-P soil, the barley yield showed little response to soil amendment, even at 300–500 kg P ha{sup −1} application, although the barley took up more P at higher applications. The apparent P use efficiency of the additive was 20% in ryegrass - much higher than that of barley for which P use efficiencies varied on the two soils. Generally, crop Cd concentrations were little affected by the increasing and high applications of ash, except for relatively high Cd concentrations in barley after applying 25 Mg ha{sup −1} straw ash. Contrarily, even modest increases in the TSP application markedly increased Cd uptake in plants. This might be explained by the low Cd solubility in the ash or by the reduced Cd availability due to the liming effect of ash. High concentrations of resin-extractable P (available P) in the ash-amended soil after harvest indicate that the ash may also contribute to P availability for the following crops. In conclusion, the biomass ashes in this study had P availability similar to the TSP fertiliser and did not contaminate the crop with Cd during the first year. - Highlights: • Effects of four biomass ashes vs. a P fertiliser (TSP) on two crops were studied. • Ashes increased crop yields with P availability similar to TSP on P-depleted soil

  13. Microsoft Exchange Server 2013 high availability

    CERN Document Server

    Mota, Nuno

    2014-01-01

    This book is a hands-on practical guide that provides the reader with a number of clear scenarios and examples, making it easier to understand and apply the new concepts. Each chapter can be used as a reference, or it can be read from beginning to end, allowing consultants/administrators to build a solid and highly available Exchange 2013 environment. If you are a messaging professional who wants to learn to design a highly available Exchange 2013 environment, this book is for you. Although not a definite requirement, practical experience with Exchange 2010 is expected, without being a subject

  14. Enabling High Data Availability in a DHT

    NARCIS (Netherlands)

    Knezevic, Predrag; Wombacher, Andreas; Risse, Thomas

    Many decentralized and peer-to-peer applications require some sort of data management. Besides P2P file-sharing, there are already scenarios (e.g. BRICKS project) that need management of finer-grained objects including updates and, keeping them highly available in very dynamic communities of peers.

  15. Discovery of a bipolar and highly variable mass outflow from the symbiotic binary StHa 190

    CERN Document Server

    Munari, U; Yudin, B F; Marrese, P M; Zwitter, T; Gratton, R G; Bonanno, G; Bruno, P; Cali, A; Claudi, R U; Cosentino, R; Desidera, S; Farisato, G; Martorana, G; Marino, G; Rebeschini, M; Scuderi, S; Timpanaro, M C

    2001-01-01

    A highly and rapidly variable bipolar mass outflow from StHa 190 has been discovered, the first time in a yellow symbiotic star. Permitted emission lines are flanked by symmetrical jet features and multi-component P-Cyg profiles, with velocities up to 300 km/sec. Given the high orbital inclination of the binary, if the jets leave the system nearly perpendicular to the orbital plane, the de-projected velocity equals or exceeds the escape velocity (1000 km/sec). StHa190 looks quite peculiar in many other respects: the hot component is an O-type sub-dwarf without an accretion disk or a veiling nebular continuum and the cool component is a G7 III star rotating at a spectacular 105 km/sec unseen by a large margin in field G giants.

  16. HA-Coated Implant

    DEFF Research Database (Denmark)

    Daugaard, Henrik; Søballe, Kjeld; Bechtold, Joan E

    2014-01-01

    The goal of osseointegration of orthopedic and dental implants is the rapid achievement of a mechanically stable and long lasting fixation between living bone and the implant surface. In total joint replacements of cementless designs, coatings of calcium phosphates were introduced as a means...... of improving the fixation of implants. Of these, hydroxyapatite (HA) is the most widely used and most extensively investigated. HA is highly osseoconductive, and the positive effect is well documented in both basic and long-term clinical research [1–6]. This chapter describes experimental and clinical studies...... evaluating bone-implant fixation with HA coatings....

  17. A Highly Available Grid Metadata Catalog

    DEFF Research Database (Denmark)

    Jensen, Henrik Thostrup; Kleist, Joshva

    2009-01-01

    This article presents a metadata catalog, intended foruse in grids. The catalog provides high availability, by replication across several hosts. The replicas are kept consistent using a replication protocol based on the Paxos algorithm. A majority of the replicas must be available in order...... for the system to function. The data model used in the catalog is RDF, which allows users to create theirown name spaces and schemas. Querying is performed using SPARQL. Additionally the catalog can be used as a synchronization mechanism, by utilizing a compare and swap operation. The catalog is accessed using...... HTTP with proxy certificates, and uses GACL for flexible access control.The performance of the catalog is tested in several ways, including a distributed setup between geographically separated sites....

  18. Haïti

    Directory of Open Access Journals (Sweden)

    Marc Pabois

    2012-04-01

    Full Text Available Figure 1Carte schématique de Haïti. Atelier de photogrammétrie de l’Inventaire généralMichel MaumontTrois missions ont eu lieu à Haïti, la première en 1997, les deux autres en 1998. Le Directeur du patrimoine à Haïti et le Directeur de la division du patrimoine culturel à l’Unesco ont ainsi fait appel au Ministère de la Culture afin de faire bénéficier Haïti de l’expérience acquise par la France dans le domaine de l’Inventaire. L’objectif de la première mission était d’analyser avec les respo...

  19. Digitally Controlled High Availability Power Supply

    Energy Technology Data Exchange (ETDEWEB)

    MacNair, David; /SLAC

    2009-05-07

    This paper will report on the test results of a prototype 1320 watt power module for a high availability power supply. The module will allow parallel operation for N+1 redundancy with hot swap capability. The two quadrant output of each module allows pairs of modules to provide a 4 quadrant (bipolar) operation. Each module employs a novel 4 FET buck regulator arranged in a bridge configuration. Each side of the bridge alternately conducts through a small saturable ferrite that limits the reverse current in the FET body diode during turn off. This allows hard switching of the FETs with low switching losses. The module is designed with over-rated components to provide high reliability and better then 97% efficiency at full load. The modules use a Microchip DSP for control, monitoring, and fault detection. The switching FETS are driven by PWM modules in the DSP at 60 KHz. A Dual CAN bus interface provides for low cost redundant control paths. The DSP will also provide current sharing between modules, synchronized switching, and soft start up for hot swapping. The input and output of each module have low resistance FETs to allow hot swapping and isolation of faulted units.

  20. Cytoprotective responses in HaCaT keratinocytes exposed to high doses of curcumin

    NARCIS (Netherlands)

    Lundvig, D.M.S.; Pennings, S.W.C.; Brouwer, K.M.; Mtaya-Mlangwa, M.; Mugonzibwa, E.; Kuijpers-Jagtman, A.M.; Wagener, F.A.D.T.G.; Hoff, J.W. Von den

    2015-01-01

    Wound healing is a complex process that involves the well-coordinated interactions of different cell types. Topical application of high doses of curcumin, a plant-derived polyphenol, enhances both normal and diabetic cutaneous wound healing in rodents. For optimal tissue repair interactions between

  1. Cytoprotective responses in HaCaT keratinocytes exposed to high doses of curcumin

    NARCIS (Netherlands)

    Lundvig, D.M.S.; Pennings, S.W.C.; Brouwer, K.M.; Mtaya-Mlangwa, M.; Mugonzibwa, E.; Kuijpers-Jagtman, A.M.; Wagener, F.A.D.T.G.; Hoff, J.W. Von den

    2015-01-01

    Wound healing is a complex process that involves the well-coordinated interactions of different cell types. Topical application of high doses of curcumin, a plant-derived polyphenol, enhances both normal and diabetic cutaneous wound healing in rodents. For optimal tissue repair interactions between

  2. High Available COTS Based Computer for Space

    Science.gov (United States)

    Hartmann, J.; Magistrati, Giorgio

    2015-09-01

    The availability and reliability factors of a system are central requirements of a target application. From a simple fuel injection system used in cars up to a flight control system of an autonomous navigating spacecraft, each application defines its specific availability factor under the target application boundary conditions. Increasing quality requirements on data processing systems used in space flight applications calling for new architectures to fulfill the availability, reliability as well as the increase of the required data processing power. Contrary to the increased quality request simplification and use of COTS components to decrease costs while keeping the interface compatibility to currently used system standards are clear customer needs. Data processing system design is mostly dominated by strict fulfillment of the customer requirements and reuse of available computer systems were not always possible caused by obsolescence of EEE-Parts, insufficient IO capabilities or the fact that available data processing systems did not provide the required scalability and performance.

  3. Availability Analysis for High Voltage Synchronous Motor

    Institute of Scientific and Technical Information of China (English)

    DING Jin-hua; Erland Olsson; ZHOU Rong

    2004-01-01

    The operating experience data for 34 motors running for approximately 15 years in paper plants are collected. According to the data set, the reliability and availability characteristics of a highvoltage synchronous motor are analyzed based on the Markov Model. The unit or subsystem main rotor with windings in the motor system is more important for the motor system's availability, though its failure rate is lower compared to other units. Preventive maintenance is first introduced as a state in the markov Model for the motor system.

  4. Thermal Characteristic Of Waste-Derived Hydroxyapatite (HA) Reinforced Ultra High Molecular Weight Polyethylene (UHMWPE) Composites For Fused Deposition Modeling (FDM) Process

    Science.gov (United States)

    Ansari, Mohamad Helmi Bin Md; Ibrahim, Mohd Halim Irwan Bin

    2017-01-01

    The present study provides a hydrothermal synthesis to obtain Hydroxyapatite (HA) powder from waste eggshells. This waste-derived HA has been characterized by X-ray diffraction, scanning electron microscopy and energy dispersive spectroscopy analysis. Waste-derived HA will be reinforced the Ultra-High Molecular Weight Polyethylene (UHMWPE) to develop a material composite for biomedical applications because of impressive mechanical properties owned by UHMWPE. Main challenger is UHMWPE has an ultra-high viscosity that renders continuous melt- state processes including one of the additive manufacturing processes which is Fused Deposition Modeling (FDM). To develop this material as feedstock in FDM process, it has been overcome by blending UHMWPE with waste-derived HA as filler. It exhibit the inclusion of 50wt% HA has reduced the degradation temperature in TGA and DSC thus enhances the processability in FDM process.

  5. PostgreSQL 9 high availability cookbook

    CERN Document Server

    Thomas, Shaun M

    2014-01-01

    A comprehensive series of dependable recipes to design, build, and implement a PostgreSQL server architecture free of common pitfalls that can operate for years to come. Each chapter is packed with instructions and examples to simplify even highly complex database operations. If you are a PostgreSQL DBA working on Linux systems who want a database that never gives up, this book is for you. If you've ever experienced a database outage, restored from a backup, spent hours trying to repair a malfunctioning cluster, or simply want to guarantee system stability, this book is definitely for you.

  6. A lightweight high availability strategy for Atlas LCG File Catalogs

    Energy Technology Data Exchange (ETDEWEB)

    Martelli, Barbara; Salvo, Alessandro de; Anzellotti, Daniela; Rinaldi, Lorenzo; Cavalli, Alessandro; Pra, Stefano dal; Dell' Agnello, Luca; Gregori, Daniele; Prosperini, Andrea; Ricci, Pier Paolo; Sapunenko, Vladimir, E-mail: barbara.martelli@cnaf.infn.i [Viale Berti Pichat 6/2, Bologna I-40127 (Italy)

    2010-04-01

    The LCG File Catalog is a key component of the LHC Computing Grid middleware [1], as it contains the mapping between Logical File Names and Physical File Names on the Grid. The Atlas computing model foresees multiple local LFC housed in each Tier-1 and Tier-0, containing all information about files stored in the regional cloud. As the local LFC contents are presently not replicated anywhere, this turns out in a dangerous single point of failure for all of the Atlas regional clouds. In order to solve this problem we propose a novel solution for high availability (HA) of Oracle based Grid services, obtained by composing an Oracle Data Guard deployment and a series of application level scripts. This approach has the advantage of being very easy to deploy and maintain, and represents a good candidate solution for all Tier-2s which are usually little centres with little manpower dedicated to service operations. We also present the results of a wide range of functionality and performance tests run on a test-bed having characteristics similar to the ones required for production. The test-bed consists of a failover deployment between the Italian LHC Tier-1 (INFN - CNAF) and an Atlas Tier-2 located at INFN - Roma1. Moreover, we explain how the proposed strategy can be deployed on the present Grid infrastructure, without requiring any change to the middleware and in a way that is totally transparent to end users and applications.

  7. A lightweight high availability strategy for Atlas LCG File Catalogs

    Science.gov (United States)

    Martelli, Barbara; de Salvo, Alessandro; Anzellotti, Daniela; Rinaldi, Lorenzo; Cavalli, Alessandro; dal Pra, Stefano; dell'Agnello, Luca; Gregori, Daniele; Prosperini, Andrea; Ricci, Pier Paolo; Sapunenko, Vladimir

    2010-04-01

    The LCG File Catalog is a key component of the LHC Computing Grid middleware [1], as it contains the mapping between Logical File Names and Physical File Names on the Grid. The Atlas computing model foresees multiple local LFC housed in each Tier-1 and Tier-0, containing all information about files stored in the regional cloud. As the local LFC contents are presently not replicated anywhere, this turns out in a dangerous single point of failure for all of the Atlas regional clouds. In order to solve this problem we propose a novel solution for high availability (HA) of Oracle based Grid services, obtained by composing an Oracle Data Guard deployment and a series of application level scripts. This approach has the advantage of being very easy to deploy and maintain, and represents a good candidate solution for all Tier-2s which are usually little centres with little manpower dedicated to service operations. We also present the results of a wide range of functionality and performance tests run on a test-bed having characteristics similar to the ones required for production. The test-bed consists of a failover deployment between the Italian LHC Tier-1 (INFN - CNAF) and an Atlas Tier-2 located at INFN - Roma1. Moreover, we explain how the proposed strategy can be deployed on the present Grid infrastructure, without requiring any change to the middleware and in a way that is totally transparent to end users and applications.

  8. Performance Analysis of a Highly Available Home Agent in Mobile Networks

    Directory of Open Access Journals (Sweden)

    Abdelgadir T. Abdelgadir

    2011-01-01

    Full Text Available Problem statement: Network Mobility as a service is provided by the NEMO protocol in IPv6 environments. NEMO is an extension to MIPv6 and thus inherits the same reliability problems of MIPv6. MIPv6 is not reliable because the Home Agent (HA is a single point of failure. In order to provide real-time services for MIPv6 networks, reliability should be considered as part of any high availability solution used to deploy Mobile IPv6 networks. Approach: Many approaches have been taken to solve the problem of HA as a single point of failure. In our proposed solution, failure detection and recovery is handled by the home agent. Therefore, recovery is transparent to the mobile network. Results: In this study we opted for using HA redundancy to provide a highly available home agent solution which achieves recovery times suitable for real-time applications. Conclusion: The results show that achieving high availability in IPv6 based mobile networks which support NEMO is possible.

  9. Development of high-availability ATCA/PCIe data acquisition instrumentation

    Energy Technology Data Exchange (ETDEWEB)

    Correia, Miguel; Sousa, Jorge; Batista, Antonio J.N.; Combo, Alvaro; Santos, Bruno; Rodrigues, Antonio P.; Carvalho, Paulo F.; Goncalves, Bruno [Instituto de Plasmas e Fusao Nuclear, Instituto Superior Tecnico, Universidade de Lisboa, 1049- 001 Lisboa (Portugal); Correia, Carlos M.B.A. [Centro de Instrumentacao, Dept. de Fisica, Universidade de Coimbra, 3004-516 Coimbra (Portugal)

    2015-07-01

    Latest Fusion energy experiments envision a quasi-continuous operation regime. In consequence, the largest experimental devices, currently in development, specify high-availability (HA) requirements for the whole plant infrastructure. HA features enable the whole facility to perform seamlessly in the case of failure of any of its components, coping with the increasing duration of plasma discharges (steady-state) and assuring safety of equipment, people, environment and investment. IPFN developed a control and data acquisition system, aiming for fast control of advanced Fusion devices, which is thus required to provide such HA features. The system is based on in-house developed Advanced Telecommunication Computing Architecture (ATCA) instrumentation modules - IO blades and data switch blades, establishing a PCIe network on the ATCA shelf's back-plane. The data switch communicates to an external host computer through a PCIe data network. At the hardware management level, the system architecture takes advantage of ATCA native redundancy and hot swap specifications to implement fail-over substitution of IO or data switch blades. A redundant host scheme is also supported by the ATCA/PCIe platform. At the software level, PCIe provides implementation of hot plug services, which translate the hardware changes to the corresponding software/operating system devices. The paper presents how the ATCA and PCIe based system can be setup to perform with the desired degree of HA, thus being suitable for advanced Fusion control and data acquisition systems. (authors)

  10. Immune escape mutants of Highly Pathogenic Avian Influenza H5N1 selected using polyclonal sera: identification of key amino acids in the HA protein.

    Directory of Open Access Journals (Sweden)

    Ioannis Sitaras

    Full Text Available Evolution of Avian Influenza (AI viruses--especially of the Highly Pathogenic Avian Influenza (HPAI H5N1 subtype--is a major issue for the poultry industry. HPAI H5N1 epidemics are associated with huge economic losses and are sometimes connected to human morbidity and mortality. Vaccination (either as a preventive measure or as a means to control outbreaks is an approach that splits the scientific community, due to the risk of it being a potential driving force in HPAI evolution through the selection of mutants able to escape vaccination-induced immunity. It is therefore essential to study how mutations are selected due to immune pressure. To this effect, we performed an in vitro selection of mutants from HPAI A/turkey/Turkey/1/05 (H5N1, using immune pressure from homologous polyclonal sera. After 42 rounds of selection, we identified 5 amino acid substitutions in the Haemagglutinin (HA protein, most of which were located in areas of antigenic importance and suspected to be prone to selection pressure. We report that most of the mutations took place early in the selection process. Finally, our antigenic cartography studies showed that the antigenic distance between the selected isolates and their parent strain increased with passage number.

  11. CMS experience of running glideinWMS in High Availability mode

    CERN Document Server

    Sfiligoi, Igor; Belforte, Stefano; Mc Crea, Alison Jean; Larson, Krista Elaine; Zvada, Marian; Holzman, Burt; P Mhashilkar; Bradley, Daniel Charles; Saiz Santos, Maria Dolores; Fanzago, Federica; Gutsche, Oliver; Martin, Terrence; Wuerthwein, Frank Karl

    2013-01-01

    The CMS experiment at the Large Hadron Collider is relying on the HTCondor-based glideinWMS batch system to handle most of its distributed computing needs. In order to minimize the risk of disruptions due to software and hardware problems, and also to simplify the maintenance procedures, CMS has set up its glideinWMS instance to use most of the attainable High Availability (HA) features. The setup involves running services distributed over multiple nodes, which in turn are located in several physical locations, including Geneva, Switzerland, Chicago, Illinois and San Diego, California. This paper describes the setup used by CMS, the HA limits of this setup, as well as a description of the actual operational experience spanning many months.

  12. Design and reliability, availability, maintainability, and safety analysis of a high availability quadruple vital computer system

    Institute of Scientific and Technical Information of China (English)

    Ping TAN; Wei-ting HE; Jia LIN; Hong-ming ZHAO; Jian CHU

    2011-01-01

    With the development of high-speed railways in China,more than 2000 high-speed trains will be put into use.Safety and efficiency of railway transportation is increasingly important.We have designed a high availability quadruple vital computer (HAQVC) system based on the analysis of the architecture of the traditional double 2-out-of-2 system and 2-out-of-3 system.The HAQVC system is a system with high availability and safety,with prominent characteristics such as fire-new internal architecture,high efficiency,reliable data interaction mechanism,and operation state change mechanism.The hardware of the vital CPU is based on ARM7 with the real-time embedded safe operation system (ES-OS).The Markov modeling method is designed to evaluate the reliability,availability,maintainability,and safety (RAMS) of the system.In this paper,we demonstrate that the HAQVC system is more reliable than the all voting triple modular redundancy (AVTMR) system and double 2-out-of-2 system.Thus,the design can be used for a specific application system,such as an airplane or high-speed railway system.

  13. Highly Available DHTs: Keeping Data Consistency After Updates

    NARCIS (Netherlands)

    Knežević, P.; Wombacher, Andreas; Risse, T.

    2006-01-01

    The research in the paper is motivated by building a decentralized/P2P XML storage on top of a DHT (Distributed Hash Table). The storage must provide high data availability and support updates. High data availability in a DHT can be guaranteed by data replication. However, DHTs can not provide a

  14. Evaluation of a conserved HA274-288 epitope to detect antibodies to highly pathogenic avian influenza virus H5N1 in Indonesian commercial poultry.

    Science.gov (United States)

    Wawegama, Nadeeka K; Tarigan, Simson; Indriani, Risa; Selleck, Paul; Adjid, Rm Abdul; Syafriati, Tati; Hardiman; Durr, Peter A; Ignjatovic, Jagoda

    2016-08-01

    A peptide enzyme linked immunosorbent assay (ELISA) based on an epitope in the haemagglutinin (HA) of avian influenza virus H5N1, amino acid positions 274-288 (HA274-288) was evaluated for detection of H5N1-specific antibodies. An optimized ELISA based on the tetrameric form of the HA274-288 epitope designated MP15 gave low background with non-immune chicken sera and detected vaccinated and infected birds. The HA274-288 epitope was highly conserved in Indonesian H5N1 strains and antibody responses were detected in the majority of the vaccinated chickens regardless of the H5N1 strain used for vaccination. The HA274-288 epitope was also conserved in the majority of H5N1 strains from the neighbouring Asian region, and other H5 subtypes potentially allowing for a wider use of the MP15 ELISA in H5N1 vaccinated and infected flocks. The MP15 ELISA results correlated significantly with haemagglutination inhibition (HI) test results and test sensitivity and specificity were 87% and 92%, respectively. The MP15 ELISA titres were significantly higher than the HI titres in all immune sera allowing for sera to be tested at a single dilution of 1:400 which is of advantage in routine surveillance. The study indicated that the MP15 ELISA is potentially useful for serological detection of H5N1 vaccinated or infected poultry and to have some advantages over the standard HI test for routine monitoring of flocks' immunity after vaccination.

  15. Mishima jo ha kyu

    Directory of Open Access Journals (Sweden)

    Matteo Casari

    2012-01-01

    Full Text Available Abstract – IT La grandezza artistica di Mishima si è espressa attraverso una eterogenea gamma di linguaggi tra i quali il teatro – nella drammaturgia ma anche nella saggistica, regia, recitazione e direzione di compagnia – ha avuto un ruolo di primo piano. Al pari di un uomo di scena Mishima inaugura, a partire dagli anni ’50, un processo di attenzione al proprio corpo come possibile, anzi necessario, veicolo di estrinsecazione etico-estetica tanto da poter istituire l’equazione corpo-teatro quale snodo profondo del suo processo creativo. Il corpo sognato e ottenuto da Mishima attraverso l’addestramento nel kendo, in altre arti marziali e nel body building si conformava ad un ideale estetico di matrice greco-classica assai lontano dal corpo teatrale nipponico. Il corpo come luogo di elaborazione e strumento di espressione autentica da realizzare con impegno, però, lo legano profondamente alle esperienze nascenti – tra gli anni ’50 e ’60 – delle avanguardie teatrali giapponesi. La metafora teatrale è spesso usata nella lettura critica del Mishima uomo e artista con accezione deteriore: un personaggio che dà spettacolo di sé con ripetute provocazioni tra le quali il suicidio del 25 novembre 1970 non sarebbe che l’esempio ultimo e più estremo. La costruzione di sé come personaggio, invece, sembrerebbe corrispondere ad una ben più profonda e meditata necessità di comporre la propria vita in una sapiente messa in scena di classica perfezione: i principi della scansione ritmico formale del jo ha kyu, pilastro teorico del teatro no codificato da Zeami tra XIV e XV secolo, offrono un valido modello di riferimento. Abstract – EN Mishima’s artistic greatness has been expressed through a diverse range of languages among wich theater – in dramaturgy as well as in written essays, as a director, performer and in company direction – played a central role. As a true front-man, Mishima – starting from the 50’s

  16. Síndrome HaNDL / HaNDL Syndrome / Síndrome HaNDL

    Directory of Open Access Journals (Sweden)

    Camilo Ernesto Barros-Gutiérrez, MD., Esp.

    2015-03-01

    Full Text Available Introducción: El Síndrome HaNDL (Headache and neurologic deficits with cerebroespinal fluid lymphocytosis por sus siglas en inglés, es una patología que cursa con cefalea, focalización neurológica y linfocitosis en el líquido cefalorraquídeo. Objetivo: este artículo busca presentar un caso de Síndrome de HaNDL, puesto que esta condición nosológica implica un reto diagnóstico. Resultados y conclusiones: Se presenta el caso de un paciente con diagnóstico de síndrome de HaNDL los hallazgos al examen físico y del líquido cefalorraquídeo. [Barros-Gutiérrez CE, Silva-Monsalve E, Gualtero-Trujillo S. Síndrome HaNDL. MedUNAB 2015; 17(3: xx-xx]. Introduction: The syndrome of transient Headache and Neurological Deficits with cerebrospinal fluid Lymphocytosis, is a pathology that presents cephalalgia, neurological focalization and lymphocytes in the cerebrospinal fluid. Objective: This article presents a HaNDL Syndrome case, since this nosological condition implies a diagnostic challenge. Results and conclusions: A case of a patient with HaNDL syndrome diagnosis, physical examination findings and cerebrospinal fluid are presented. [Barros-Gutiérrez CE, Silva-Monsalve E, Gualtero-Trujillo S. HaNDL Syndrome. MedUNAB 2015; 17(3: xx-xx]. Introdução: A síndrome de HaNDL (Headache and neurologic déficits with cerebrospinal fluid lymphocytosis por sua sigla em inglês é uma doença que causa dor de cabeça, foco neurológico e linfocitose no líquido cefalorraquidiano. Objetivo: Este artigo tem como objetivo apresentar um caso de Síndrome de HaNDL, uma vez que esta condição nosológica envolve um desafio diagnóstico. Resultados e conclusões: Apresenta-se o caso de um paciente diagnosticado com a síndrome de HaNDL, o encontrado no exame físico e o líquido cefalorraquidiano. [Barros-Gutiérrez CE, Silva-Monsalve E, Gualtero-Trujillo S. Síndrome HaNDL. MedUNAB 2015; 17(3: xx-xx].

  17. High plant availability of phosphorus and low availability of cadmium in four biomass combustion ashes

    DEFF Research Database (Denmark)

    Li, Xiaoxi; Rubæk, Gitte Holton; Sørensen, Peter

    2016-01-01

    For biomass combustion to become a sustainable energy production system, it is crucial to minimise landfill of biomass ashes, to recycle the nutrients and to minimise the undesirable impact of hazardous substances in the ash. In order to test the plant availability of phosphorus (P) and cadmium (Cd......) in four biomass ashes, we conducted two pot experiments on a P-depleted soil and one mini-plot field experiment on a soil with adequate P status. Test plants were spring barley and Italian ryegrass. Ash applications were compared to triple superphosphate (TSP) and a control without P application. Both TSP...... ash. Contrarily, even modest increases in the TSP application markedly increased Cd uptake in plants. This might be explained by the low Cd solubility in the ash or by the reduced Cd availability due to the liming effect of ash. High concentrations of resin-extractable P (available P) in the ash...

  18. Model and Implementation of Communication Link Management Supporting High Availability

    Institute of Scientific and Technical Information of China (English)

    Luo Juan; Cao Yang; He Zheng; Li Feng

    2004-01-01

    Despite the rapid evolution in all aspects of computer technology, both the computer hardware and software are prone to numerous failure conditions. In this paper, we analyzed the characteristic of a computer system and the methods of constructing a system , proposed a communication link management model supporting high availability for network applications, Which will greatly increase the high availability of network applications. Then we elaborated on heartbeat or service detect, fail-over, service take-over, switchback and error recovery process of the model. In the process of constructing the communication link, we implemented the link management and service take-over with high availability requirement, and discussed the state and the state transition of building the communication link between the hosts, depicted the message transfer and the start of timer. At Last, we applied the designed high availability system to a network billing system, and showed how the system was constructed and implemented, which perfectly satisfied the system requirements.

  19. New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization.

    Science.gov (United States)

    Klimek, Katarzyna; Przekora, Agata; Pałka, Krzysztof; Ginalska, Grażyna

    2016-10-01

    Recent studies have shown that thermal method for β-1,3-glucan (curdlan) gelation performed at temperature above 80°C enables fabrication of biocompatible bone scaffolds. The aim of this study was to establish new method for fabrication of β-1,3-glucan/hydroxyapatite (glu/HA) scaffold using ion-exchanging dialysis for curdlan gelation that allows for the modifications of the glu/HA material with thermo-sensitive agents like growth factors or adhesive proteins. Obtained results reveal that fabricated scaffold appears to be highly osteoconductive as it is nontoxic, promotes osteoblast growth and proliferation as well as increases bone alkaline phosphatase level thereby enhancing cell differentiation. It was demonstrated that developed new method for the glu/HA scaffold fabrication allows to obtain material that not only can be modified with thermo-sensitive agents at the stage of production process but also is a promising candidate for bone tissue engineering applications to act as a framework for osteoblasts to spread and form new bone. It should be noted that dialysis method for curdlan gelation has never been used before to fabricate bone scaffold. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2528-2536, 2016.

  20. Intranasal vaccination with a plant-derived H5 HA vaccine protects mice and ferrets against highly pathogenic avian influenza virus challenge.

    Science.gov (United States)

    Major, Diane; Chichester, Jessica A; Pathirana, Rishi D; Guilfoyle, Kate; Shoji, Yoko; Guzman, Carlos A; Yusibov, Vidadi; Cox, Rebecca J

    2015-01-01

    Highly pathogenic avian influenza H5N1 infection remains a public health threat and vaccination is the best measure of limiting the impact of a potential pandemic. Mucosal vaccines have the advantage of eliciting immune responses at the site of viral entry, thereby preventing infection as well as further viral transmission. In this study, we assessed the protective efficacy of hemagglutinin (HA) from the A/Indonesia/05/05 (H5N1) strain of influenza virus that was produced by transient expression in plants. The plant-derived vaccine, in combination with the mucosal adjuvant (3',5')-cyclic dimeric guanylic acid (c-di-GMP) was used for intranasal immunization of mice and ferrets, before challenge with a lethal dose of the A/Indonesia/05/05 (H5N1) virus. Mice vaccinated with 15 μg or 5 μg of adjuvanted HA survived the viral challenge, while all control mice died within 10 d of challenge. Vaccinated animals elicited serum hemagglutination inhibition, IgG and IgA antibody titers. In the ferret challenge study, all animals vaccinated with the adjuvanted plant vaccine survived the lethal viral challenge, while 50% of the control animals died. In both the mouse and ferret models, the vaccinated animals were better protected from weight loss and body temperature changes associated with H5N1 infection compared with the non-vaccinated controls. Furthermore, the systemic spread of the virus was lower in the vaccinated animals compared with the controls. Results presented here suggest that the plant-produced HA-based influenza vaccine adjuvanted with c-di-GMP is a promising vaccine/adjuvant combination for the development of new mucosal influenza vaccines.

  1. Approach to Achieve High Availability in Critical Infrastructure

    Science.gov (United States)

    2015-09-01

    Regardless, current technology includes RFID tags for systems, and tablets can be implemented to assist in real-time updates. Current Wi-Fi technology can...APPROACH TO ACHIEVE HIGH AVAILABILITY IN CRITICAL INFRASTRUCTURE Yew Kee Hoo Senior Engineer, Defence Science and Technology Agency, Singapore B.E...Between Failure MTTF Mean Time to Repair NAVFAC Naval Facilities Engineering Command xvi O&M Operations and Maintenance RFID Radio Frequency

  2. A High-Availability, Distributed Hardware Control System Using Java

    Science.gov (United States)

    Niessner, Albert F.

    2011-01-01

    Two independent coronagraph experiments that require 24/7 availability with different optical layouts and different motion control requirements are commanded and controlled with the same Java software system executing on many geographically scattered computer systems interconnected via TCP/IP. High availability of a distributed system requires that the computers have a robust communication messaging system making the mix of TCP/IP (a robust transport), and XML (a robust message) a natural choice. XML also adds the configuration flexibility. Java then adds object-oriented paradigms, exception handling, heavily tested libraries, and many third party tools for implementation robustness. The result is a software system that provides users 24/7 access to two diverse experiments with XML files defining the differences

  3. A High-Availability, Distributed Hardware Control System Using Java

    Science.gov (United States)

    Niessner, Albert F.

    2011-01-01

    Two independent coronagraph experiments that require 24/7 availability with different optical layouts and different motion control requirements are commanded and controlled with the same Java software system executing on many geographically scattered computer systems interconnected via TCP/IP. High availability of a distributed system requires that the computers have a robust communication messaging system making the mix of TCP/IP (a robust transport), and XML (a robust message) a natural choice. XML also adds the configuration flexibility. Java then adds object-oriented paradigms, exception handling, heavily tested libraries, and many third party tools for implementation robustness. The result is a software system that provides users 24/7 access to two diverse experiments with XML files defining the differences

  4. High hydrostatic pressure effects on Listeria monocytogenes and L. innocua: Evidence for variability in inactivation behaviour and in resistance to pediocin bacHA-6111-2.

    Science.gov (United States)

    Bruschi, Carolina; Komora, Norton; Castro, Sónia Marília; Saraiva, Jorge; Ferreira, Vânia Borges; Teixeira, Paula

    2017-06-01

    The effect of high hydrostatic pressure (HHP) on the survival of 14 strains of Listeria monocytogenes from food or clinical origins, selected to represent different pheno and genotypes, was evaluated. Stationary phase cells were submitted to 300, 400 and 500 MPa at 10 °C, for 5 min. A high variability in the resistance of L. monocytogenes to pressure was observed, and particularly two strains isolated from food were significantly more baroresistant than the rest. Strains of L. monocytogenes resistant to one or more antibiotics exhibited significantly higher levels of survival after the high pressure treatment at 400 MPa. No correlation was found between strains' origin or thermal tolerance and resistance to HHP. The suitability of two strains of L. innocua as surrogates of L. monocytogenes, was also investigated. These exhibited significantly higher sensitivities to HHP than observed for some L. monocytogenes. The antimicrobial effect of the antilisterial bacteriocin (bacHA-6111-2) increased after L. monocytogenes cells had been exposed to pressure. The data obtained underlines the importance of strain selection for studies aiming to evaluate HHP efficacy to ensure safety of HHP-treated foods. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. High Availability Solution: Resource Usage Management in Virtualized Software Aging

    Directory of Open Access Journals (Sweden)

    Aye Myat Myat Paing

    2012-07-01

    Full Text Available As businesses increasingly rely on IT for their mission-critical operations, continuous availability is auniversal concern. Nowadays, virtualized platform has become the popular option to deploy complexenough services. Deployed services are expected to be always, available, but these long running servicesare especially sensitive to suffer from software aging phenomenon. Software aging of virtual machinemonitors (VMMs is becoming critical because performance degradation or crash failure of a VMM affectsall virtual machines (VMs on it. To counteract this issue, we deploy software rejuvenation methodology.To eliminate the service outage during software rejuvenation process, we combine the rejuvenation withlive migration technology. Live VM migration enables a running VM on a host serer to move onto the otherhost server with very small interruption of the execution. Live VM migration depends on VMs placementand efficient resource available is required. The idea behind our paper is two-fold. First, we present theoptimization of the resource usage as accepting as many services as in virtualized environment whichsupport of VM live migration. Second, to demonstrate how much it can improve system availability, thestochastic Petri nets model a virtualized server system in case of using time based software rejuvenation forVMM is presented. Finally, we perform the numerical analysis to evaluate the model.

  6. Condom availability in high risk places and condom use

    DEFF Research Database (Denmark)

    Sandøy, Ingvild Fossgard; Blystad, Astrid; Shayo, Elizabeth H.;

    2012-01-01

    and one in Zambia. The study was based on a triangulation of data collection methods in the three study districts: surveys in venues where people meet new sexual partners, population-based surveys and focus group discussions. The data was collected within an overall study on priority setting in health......Background A number of studies from countries with severe HIV epidemics have found gaps in condom availability, even in places where there is a substantial potential for HIV transmission. Although reported condom use has increased in many African countries, there are often big differences...... by socioeconomic background. The aim of this study was to assess equity aspects of condom availability and uptake in three African districts to evaluate whether condom programmes are given sufficient priority. Methods Data on condom availability and use was examined in one district in Kenya, one in Tanzania...

  7. Morphological Effects of HA on the Cell Compatibility of Electrospun HA/PLGA Composite Nanofiber Scaffolds

    Directory of Open Access Journals (Sweden)

    Adnan Haider

    2014-01-01

    Full Text Available Tissue engineering is faced with an uphill challenge to design a platform with appropriate topography and suitable surface chemistry, which could encourage desired cellular activities and guide bone tissue regeneration. To develop such scaffolds, composite nanofiber scaffolds of nHA and sHA with PLGA were fabricated using electrospinning technique. nHA was synthesized using precipitation method, whereas sHA was purchased. The nHA and sHA were suspended in PLGA solution separately and electrospun at optimized electrospinning parameters. The composite nanofiber scaffolds were characterized by FE-SEM, EDX analysis, TEM, XRD analysis, FTIR, and X-ray photoelectron. The potential of the HA/PLGA composite nanofiber as bone scaffolds in terms of their bioactivity and biocompatibility was assessed by culturing the osteoblastic cells onto the composite nanofiber scaffolds. The results from in vitro studies revealed that the nHA/PLGA composite nanofiber scaffolds showed higher cellular adhesion, proliferation, and enhanced osteogenesis performance, along with increased Ca+2 ions release compared to the sHA/PLGA composite nanofiber scaffolds and pristine PLGA nanofiber scaffold. The results show that the structural dependent property of HA might affect its potential as bone scaffold and implantable materials in regenerative medicine and clinical tissue engineering.

  8. Architecture & Design of Affordable and Highly Available Enterprise Cloud Service

    Directory of Open Access Journals (Sweden)

    Rabi Prasad Padhy

    2013-01-01

    Full Text Available Cloud computing is a way to increase capacity or add capabilities on the fly without investing in additional infrastructure, licensing additional software or training new personnel. Enterprises must embrace this bleeding edge real-time business model in order to compete in the current marketplace. Deployment of enterprise applications in public cloud can reduce investment on IT infrastructure both in terms of hardware and software, whenever new services are to be provisioned. Furthermore, cloud services are quite attractive to business because of its dynamic scalability, privacy, performance and ability to handle heterogeneous environments. Instead of spending a lot of time in figuring out server setup and working on routers, it is judicious to subscribe cloud based 24/7 support and affordable services. It’s an extremely important consideration to subscribe to at least two cloud vendors for smooth running of applications and ensure application availability by switching from one provider to another with minimal management effort. In this article we have proposed an architecture and design of a cost affordable online enterprise cloud application that subscribes to two public cloud vendors with respect to cost and availability parameters. We also present the reasons for the current trend of enterprises moving towards 24/7 online business cloud services.

  9. Prospects of HA-Based Universal Influenza Vaccine

    Directory of Open Access Journals (Sweden)

    Anwar M. Hashem

    2015-01-01

    Full Text Available Current influenza vaccines afford substantial protection in humans by inducing strain-specific neutralizing antibodies (Abs. Most of these Abs target highly variable immunodominant epitopes in the globular domain of the viral hemagglutinin (HA. Therefore, current vaccines may not be able to induce heterosubtypic immunity against the divergent influenza subtypes. The identification of broadly neutralizing Abs (BnAbs against influenza HA using recent technological advancements in antibody libraries, hybridoma, and isolation of single Ab-secreting plasma cells has increased the interest in developing a universal influenza vaccine as it could provide life-long protection. While these BnAbs can serve as a source for passive immunotherapy, their identification represents an important step towards the design of such a universal vaccine. This review describes the recent advances and approaches used in the development of universal influenza vaccine based on highly conserved HA regions identified by BnAbs.

  10. Does High Educational Attainment Limit the Availability of Romantic Partners?

    Science.gov (United States)

    Burt, Isaac; Lewis, Sally V.; Beverly, Monifa G.; Patel, Samir H.

    2010-01-01

    Research indicates that highly educated individuals endure hardships in finding suitable romantic partners. Romantic hardships affect social and emotional adjustment levels, leading to low self-efficacy in relationship decision making. To address the need for research pertaining to this topic, the authors explored the experiences of eight…

  11. Condom availability in high risk places and condom use

    DEFF Research Database (Denmark)

    Sandøy, Ingvild Fossgard; Blystad, Astrid; Shayo, Elizabeth H.

    2012-01-01

    study findings indicate that substantial further efforts should be made to secure that condoms are easily accessible in places where sexual relationships are initiated. Although condom distribution in drinking places has been pinpointed in the HIV/AIDS prevention strategies of all the three countries......Background A number of studies from countries with severe HIV epidemics have found gaps in condom availability, even in places where there is a substantial potential for HIV transmission. Although reported condom use has increased in many African countries, there are often big differences...... and one in Zambia. The study was based on a triangulation of data collection methods in the three study districts: surveys in venues where people meet new sexual partners, population-based surveys and focus group discussions. The data was collected within an overall study on priority setting in health...

  12. Intermonomer Interactions in Hemagglutinin Subunits HA1 and HA2 Affecting Hemagglutinin Stability and Influenza Virus Infectivity

    Science.gov (United States)

    DeFeo, Christopher J.; Alvarado-Facundo, Esmeralda; Vassell, Russell

    2015-01-01

    ABSTRACT Influenza virus hemagglutinin (HA) mediates virus entry by binding to cell surface receptors and fusing the viral and endosomal membranes following uptake by endocytosis. The acidic environment of endosomes triggers a large-scale conformational change in the transmembrane subunit of HA (HA2) involving a loop (B loop)-to-helix transition, which releases the fusion peptide at the HA2 N terminus from an interior pocket within the HA trimer. Subsequent insertion of the fusion peptide into the endosomal membrane initiates fusion. The acid stability of HA is influenced by residues in the fusion peptide, fusion peptide pocket, coiled-coil regions of HA2, and interactions between the surface (HA1) and HA2 subunits, but details are not fully understood and vary among strains. Current evidence suggests that the HA from the circulating pandemic 2009 H1N1 influenza A virus [A(H1N1)pdm09] is less stable than the HAs from other seasonal influenza virus strains. Here we show that residue 205 in HA1 and residue 399 in the B loop of HA2 (residue 72, HA2 numbering) in different monomers of the trimeric A(H1N1)pdm09 HA are involved in functionally important intermolecular interactions and that a conserved histidine in this pair helps regulate HA stability. An arginine-lysine pair at this location destabilizes HA at acidic pH and mediates fusion at a higher pH, while a glutamate-lysine pair enhances HA stability and requires a lower pH to induce fusion. Our findings identify key residues in HA1 and HA2 that interact to help regulate H1N1 HA stability and virus infectivity. IMPORTANCE Influenza virus hemagglutinin (HA) is the principal antigen in inactivated influenza vaccines and the target of protective antibodies. However, the influenza A virus HA is highly variable, necessitating frequent vaccine changes to match circulating strains. Sequence changes in HA affect not only antigenicity but also HA stability, which has important implications for vaccine production, as well

  13. HA/Ti composite for biomedical application by mechanical milling

    Institute of Scientific and Technical Information of China (English)

    刘咏; 刘芳; 周科朝; 黄伯云

    2003-01-01

    In order to overcome the poor mechanical properties of HA and the low bioactivity of Ti, HA/Ti com-posites with various compositions were prepared by mechanical milling. The effects of milling condition and the com-position on the microstructure, the density and the hardness of the composites were studied. The results show thatduring the ball milling process, Ti particles are refined and the homogeneity of the HA/Ti mixtures is improved;HA will partially decompose due to the existence of Ti and high sintering temperature. The microstructure of HA/Ti composites is highly dependent on the milling condition and the composition. In the microstructure, Ti phase con-nects to be a continuous network, and HA/Ti mixtures disperse in the network. The longer the milling time, the fi-ner the network will be. The density of HA/Ti composites decreases with the content of HA increasing and themilling time prolonging, because HA deteriorates the sinterability of Ti. The hardness of HA/Ti composites increa-ses firstly with the content of HA increasing, and then drops when the content of HA exceeds 30%. Addition ofHA will strengthen the HA/Ti composite but will decrease the density of the composite, which accounts for theeffect of HA on the hardness of the composites.

  14. Enhanced Interfacial Adhesion in HDPE/HA Composites by Surface Modification of HA Particles via in situ Polymerization and Copolymerization

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Hydroxyapatite ( HA )-reinforced high density polyethylene (HDPE) was developed as a bone replacement material. In order to enhance the interfacial bondiag between HA and polyethylene and improve the mechanical properties of HDPE/ HA composites, the surface of the micron-sized HA particles was modified by in situ polymerization of butyl acrylate ( BA ) and in situ copolynerization of vinyl triethoxyl silane (VTES) and BA ,then the modified HA particles were compounded with HDPE. The effects of the surface modification of HA on morphology and mechanical properties of HDPE/ HA composites were investigated. The experimental results show that the presence of HA particles does not inhibit the polymerization of BA . The poly( butyl acrylate) ( PBA ) segments on the HA surface enhance the compatibility between HA and HDPE, improve the dispersion of HA particles in HDPE matrix, and enhance the interfacial ndhesion between HA and matrix. Surface modifications, especially by in situ copolymerization of VTES and BA , significantly increase notch impact strengths and marginal stiffness and tensile strengths of HDPE/ HA composites. And it is found that there is a critical thickness of PBA coating on HA particles for optimum mechanical properties of HDPE / HA composites.

  15. High Availability Cluster of Linux Based on DRBD%基于DRBD的Linux高可用集群

    Institute of Scientific and Technical Information of China (English)

    巩天宁; 周书明

    2012-01-01

    Analyzing traditional high availability cluster,designing a project based on free open-source software heartbeat and DRBD to solve traditional HA-cluster's deficiency on cost,using heartbeat to construct high availability cluster,using DRBD to synchronize data between cluster's nodes,constructing and testing an FTP HA-cluster based on this design,giving an resolution to brain-split.%文章分析了传统高可用性集群,针对其成本较高的缺点提出了使用免费开源软件heartbeat构造高可用集群、DR BD在集群节点之间同步数据以取代共享磁盘的方案,并设计使用该方案构建高可用FTP集群的实例,通过测试确认该方案的可行性,最后给出集群脑裂的解决方法。

  16. Influence of highly porous electrospun PLGA/PCL/nHA fibrous scaffolds on the differentiation of tooth bud cells in vitro.

    Science.gov (United States)

    Cai, Xinjie; Ten Hoopen, Sofie; Zhang, Weibo; Yi, Charles; Yang, Wanxun; Yang, Fang; Jansen, John A; Walboomers, X Frank; Yelick, Pamela C

    2017-09-01

    In this study, we investigated the use of three-dimensional electrospun poly(lactic-co-glycolic acid)/poly(ε-caprolactone) (PLGA/PCL) scaffolds seeded and cultured with postnatal dental cells, for improved dental tissue regeneration. Wet-electrospinning combined with ultrasonic treatment was studied as a method to enhance scaffold porosity and to promote cell-cell interactions. We also investigated whether nano-hydroxyapatite (nHA) incorporation could enhance dental cell differentiation. All scaffolds were seeded with human tooth pulp-derived dental mesenchymal (hDM) cells, or a combination of hDM and pig dental epithelial (pDE) cells, cultured for up to 28 days. Developmentally staged samples were assessed using scanning electron microscopy, histological, immunohistochemical, DNA and alkaline phosphatase activity assays, and quantitative-PCR for ameloblastic, odontoblastic, and osteogenic related gene expression. Results showed that electrospun scaffolds exhibited sufficient porosity to support robust cell ingrowth. Additional ultrasonic treatment led to a less homogeneous scaffold porosity, resulting in evident cell clustering and enhanced hDM-pDE cell-cell interactions. Finally, nHA incorporation was found to enhance dental cell differentiation. However, it also resulted in smaller fiber diameter and reduced scaffold porosity, and inhibited cell ingrowth and proliferation. In conclusion, ultrasonically treated wet-electrospun PLGA/PCL scaffolds are a suitable material for dental tissue engineering, and support future in vivo evaluations of this model. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2597-2607, 2017. © 2017 Wiley Periodicals, Inc.

  17. Trinh T. Minh-ha

    DEFF Research Database (Denmark)

    Nelund, Sidsel; Trinh T, Minh-ha

    2012-01-01

    Born in Vietnam, Trinh T. Minh-ha is known as a filmmaker, composer and writer. Contributing generously to these art forms, she was also a pioneer in the 1980’s theoretical conceptualisation of the Other, verbalising especially the position of women in developing countries. 
 Currently, Trinh T. ...

  18. Trinh T. Minh-ha

    DEFF Research Database (Denmark)

    Nelund, Sidsel; Trinh T, Minh-ha

    2012-01-01

    Born in Vietnam, Trinh T. Minh-ha is known as a filmmaker, composer and writer. Contributing generously to these art forms, she was also a pioneer in the 1980’s theoretical conceptualisation of the Other, verbalising especially the position of women in developing countries. 
 Currently, Trinh T....... Minh-ha holds the post of Professor of Gender and Women's Studies, and of Rhetoric at the University of California, Berkeley. She has lived, among other places, in Vietnam, USA, France, Senegal, and Japan and this geographic diversity is reflected in her work, in which several countries have been...... for marginalised groups. However, she also moves in circles of documentary, cinema, feminist studies, postcolonial theory, contemporary visual art, literature, and music composition. This interview consists of 4 parts concerning positioning, research, and media, finally coming back to positioning again....

  19. A comparative study on in vitro osteogenic priming potential of electron spun scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for tissue engineering application.

    Directory of Open Access Journals (Sweden)

    Hanumantha Rao Balaji Raghavendran

    Full Text Available A comparative study on the in vitro osteogenic potential of electrospun poly-L-lactide/hydroxyapatite/collagen (PLLA/HA/Col, PLLA/HA, and PLLA/Col scaffolds was conducted. The morphology, chemical composition, and surface roughness of the fibrous scaffolds were examined. Furthermore, cell attachment, distribution, morphology, mineralization, extracellular matrix protein localization, and gene expression of human mesenchymal stromal cells (hMSCs differentiated on the fibrous scaffolds PLLA/Col/HA, PLLA/Col, and PLLA/HA were also analyzed. The electrospun scaffolds with a diameter of 200-950 nm demonstrated well-formed interconnected fibrous network structure, which supported the growth of hMSCs. When compared with PLLA/H%A and PLLA/Col scaffolds, PLLA/Col/HA scaffolds presented a higher density of viable cells and significant upregulation of genes associated with osteogenic lineage, which were achieved without the use of specific medium or growth factors. These results were supported by the elevated levels of calcium, osteocalcin, and mineralization (P<0.05 observed at different time points (0, 7, 14, and 21 days. Furthermore, electron microscopic observations and fibronectin localization revealed that PLLA/Col/HA scaffolds exhibited superior osteoinductivity, when compared with PLLA/Col or PLLA/HA scaffolds. These findings indicated that the fibrous structure and synergistic action of Col and nano-HA with high-molecular-weight PLLA played a vital role in inducing osteogenic differentiation of hMSCs. The data obtained in this study demonstrated that the developed fibrous PLLA/Col/HA biocomposite scaffold may be supportive for stem cell based therapies for bone repair, when compared with the other two scaffolds.

  20. Lo que se ha hecho

    OpenAIRE

    Alvarado A. Víctor Hernando

    1992-01-01

    Después de transcurrido el primer módulo e iniciado el segundo, en el Curso de Postgrado en DERECHO ADMINISTRATIVO, resulta conveniente, formular algunas reflexiones sobre el avance del programa en su etapa inicial. El programa aprobado por el Instituto Colombiano para el Fomento de la Educación Superior -ICFES-, a desarrollarse en cuatro (4) MODULOS semestrales cada uno, requiriéndose dos (2) años para su cumplimiento, nos ha permitido ver que reúne las exigencias que la Universidad pretend...

  1. Lo que se ha hecho

    OpenAIRE

    1992-01-01

    Después de transcurrido el primer módulo e iniciado el segundo, en el Curso de Postgrado en DERECHO ADMINISTRATIVO, resulta conveniente, formular algunas reflexiones sobre el avance del programa en su etapa inicial. El programa aprobado por el Instituto Colombiano para el Fomento de la Educación Superior -ICFES-, a desarrollarse en cuatro (4) MODULOS semestrales cada uno, requiriéndose dos (2) años para su cumplimiento, nos ha permitido ver que reúne las exigencias que la Universidad pretend...

  2. Phylogenetic study-based hemagglutinin (HA) gene of highly pathogenic avian influenza virus (H5N1) detected from backyard chickens in Iran, 2015.

    Science.gov (United States)

    Ghafouri, Syed Ali; Langeroudi, Arash Ghalyanchi; Maghsoudloo, Hossein; Tehrani, Farshad; Khaltabadifarahani, Reza; Abdollahi, Hamed; Fallah, Mohammad Hossein

    2017-02-01

    Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype have been diversified into multiple phylogenetic clades over the past decade and are highly genetically variable. In June 2015, one outbreak of HPAI H5N1 in backyard chickens was reported in the Nogardan village of the Mazandaran Province. Tracheal tissues were taken from the dead domestic chickens (n = 10) and processed for RT-PCR. The positive samples (n = 10) were characterized as HPAI H5N1 by sequencing analysis for the hemagglutinin and neuraminidase genes. Phylogenetic analysis of the samples revealed that the viruses belonged to clade 2.3.2.1c, and cluster with the HPAI H5N1 viruses isolated from different avian species in Bulgaria, Romania, and Nigeria in 2015. They were not closely related to other H5N1 isolates detected in previous years in Iran. Our study provides new insights into the evolution and genesis of H5N1 influenza in Iran and has important implications for targeting surveillance efforts to rapidly identify the spread of the virus into and within Iran.

  3. Fabrication and evaluation of porous Ti–HA bio-nanomaterial by leaching process

    Directory of Open Access Journals (Sweden)

    A.M. Omran

    2015-05-01

    Full Text Available A porous surface of Ti–HA composite was successfully fabricated by pulsed current activated sintering (PCAS, followed by leaching using diluted H3PO4. The Ti and HA powders were mixed at different contents of the HA, Ti-5, 10, 30 and 40 wt% HA powders. The mixed powders were pressed in a coated graphite die using pulsed current activated sintering (PCAS under pressure of 60 MPa at temperature of 1000 °C for 5 min. The sintered Ti–HA specimens were immersed in the eight kinds of leaching solutions at room temperature for 24 h. The leached specimen’s surfaces were characterized using XRD, SEM, EDX and Rockwell hardness. The XRD patterns after sintering show that many phases were detected at the sintered specimen surfaces such as; Ti2O, CaO, CaTiO3, TixPy in addition to the remaining Ti and HA. Furthermore, the high concentration H3PO4 leaching solution is more efficient than the low concentration. Also the produced porous surfaces of Ti–HA materials containing more than 30% HA have a low relative density and hardness than the commercial Ti–6Al–4V ELI alloy. In a word, the presence of porous surface coated by HA will promote the nucleation of the biological apatite created with the human tissue and increase the bonding between them. So, the produced porous materials are considered so easy for the muscle cells to permeate after transplanted with high coherence.

  4. Identification and cloning of a novel isoform of mouse secretory leukocyte protease inhibitor, mSLPI-beta, overexpressed in murine leukemias and a highly liver metastatic tumor, IMC-HA1 cells.

    Science.gov (United States)

    Morita, M; Arakawa, H; Nishimura, S

    1999-01-01

    Several genes showing transcriptional alteration in a highly liver metastatic murine carcinoma cell line, IMC-HA1, were identified by mRNA differential display system. Among them, a gene identical to mSLPI was isolated as mSLPI-alpha and -beta. They were produced through an alternative splicing. Their full-length cDNA sequences were determined, and their expression in various murine tumors and normal tissues was analysed. The deduced translation product of mSLPI-alpha showed 59% identity to hSLPI. Although mSLPI-beta had the same 103-amino-acid sequence from the carboxyl terminus, the amino terminus showed hydrophilicity opposite mSLPI-alpha or hSLPI. The mSLPI-alpha was expressed ubiquitously in various tumor cell lines. Interestingly, however, mSLPI-beta expression was only observed in P388 and L1210 leukemias and IMC-HA1 cells, and in lower amounts in three normal tissues (thymus, lung and spleen), suggesting that mSLPI, and in particular the unusual splicing product, mSLPI-beta, plays a specific role in these cells, including malignant processes of tumor cells.

  5. 高可用CORBA%The Design and Realization of Highly Availability Distributed System of CORBA

    Institute of Scientific and Technical Information of China (English)

    郭乐深; 刘锦德

    2000-01-01

    Recently,while highly available computer systems used for homogeneous environment have been launched into market,those used for heterogeneous environment are still staying vendors' research labs. In this paper,a highly availability distributed system based on CORBA is proposed,and the design and implementation of this system are fully explained. The analysis results show that the scheme presented is an effective approach.

  6. Vetenfinf se ha actualizado recientemente

    Directory of Open Access Journals (Sweden)

    Veterinaria.org

    2008-02-01

    Full Text Available ResumenSe trata de un sitio web alojado en el servidor de Veterinaria.org y mantenido por el Dr. Norberto Fondevila, del Instituto de Virologia CICVyA- INTA Cautelar de Argentina .

  7. PLLA-HA composites: Synthesis and characterization

    Science.gov (United States)

    Gonzalez, Gema; Albano, Carmen; Palacios, Jordana

    2012-07-01

    A composite based on PLLA -HA was prepared by the solvent casting technique and characterized. An interaction between the polymer matrix and HA through the carbonyl and phosphate groups was obtained by FTIR . The several thermal transitions of PLLA were evaluated by DSC: the glass transition, crystallization, cold crystallization, melt-recrystallization and melting. The addition of HA to PLLA matrix increases its glass transition temperature and no major changes on the melting temperature and crystallinity were observed. The PLLA-HA composite showed better thermal stability than the neat polymer. The introduction of the nano-HA particles increased the decomposition temperature and the activation energy retarding the decomposition process.

  8. A Runtime Testability Metric for Dynamic High-Availability Component-based Systems

    NARCIS (Netherlands)

    Gonzales-Sanchez, A.; Piel, E.A.B.; Gross, H.G.; Van Gemund, A.J.C.

    2011-01-01

    Runtime testing is emerging as the solution for the integration and assessment of highly dynamic, high availability software systems where traditional development-time integration testing cannot be performed. A prerequisite for runtime testing is the knowledge about to which extent the system can be

  9. Runtime Testability in Dynamic Highly-Availability Component-based Systems

    NARCIS (Netherlands)

    Gonzalez, A.; Piel, E.; Gross, H.G.; Van Gemund, A.J.C.

    2010-01-01

    Runtime testing is emerging as the solution for the integration and assessment of highly dynamic, high availability software systems where traditional development-time integration testing cannot be performed. A prerequisite for runtime testing is the knowledge about to which extent the system can be

  10. Runtime Testability in Dynamic Highly-Availability Component-based Systems

    NARCIS (Netherlands)

    Gonzalez, A.; Piel, E.; Gross, H.G.; Van Gemund, A.J.C.

    2010-01-01

    Runtime testing is emerging as the solution for the integration and assessment of highly dynamic, high availability software systems where traditional development-time integration testing cannot be performed. A prerequisite for runtime testing is the knowledge about to which extent the system can be

  11. A Runtime Testability Metric for Dynamic High-Availability Component-based Systems

    NARCIS (Netherlands)

    Gonzales-Sanchez, A.; Piel, E.A.B.; Gross, H.G.; Van Gemund, A.J.C.

    2011-01-01

    Runtime testing is emerging as the solution for the integration and assessment of highly dynamic, high availability software systems where traditional development-time integration testing cannot be performed. A prerequisite for runtime testing is the knowledge about to which extent the system can be

  12. MySQL High Availability Tools for Building Robust Data Centers

    CERN Document Server

    Bell, Charles; Thalmann, Lars

    2010-01-01

    Server bottlenecks and failures are a fact of life in any database deployment, but they don't have to bring everything to a halt. MySQL has several features that can help you protect your system from outages, whether it's running on hardware, virtual machines, or in the cloud. MySQL High Availability explains how to use these replication, cluster, and monitoring features in a wide range of real-life situations. Written by engineers who designed many of the tools covered inside, this book reveals undocumented or hard-to-find aspects of MySQL reliability and high availability -- knowledge that

  13. TECHNICAL SUPPORT AS A BASIS OF HIGH AVAILABILITY LEVEL AND IT SYSTEM SERVICE QUALITY

    Directory of Open Access Journals (Sweden)

    Dejan Vidojevic

    2007-06-01

    Full Text Available This work presents the development and implementation methodology of technical support in IT1system operation. Methodology is developed and applied in realistic system (Information system of the Tax administration - DIS 2003, which is technically very complex and highly distributed. The results of IT system availability assessment and identification of the critical components are input parameters in the process of establishing of the technical support. The importance of technical support for achieving optimal IT system availability and IT service quality is assessed according to its operation during one year. The history of technical support system operation is a basis for further continuous improvement.

  14. High light-induced hydrogen peroxide production in Chlamydomonas reinhardtii is increased by high CO2 availability.

    Science.gov (United States)

    Roach, Thomas; Na, Chae Sun; Krieger-Liszkay, Anja

    2015-03-01

    The production of reactive oxygen species (ROS) is an unavoidable part of photosynthesis. Stress that accompanies high light levels and low CO2 availability putatively includes enhanced ROS production in the so-called Mehler reaction. Such conditions are thought to encourage O2 to become an electron acceptor at photosystem I, producing the ROS superoxide anion radical (O2·-) and hydrogen peroxide (H2 O2 ). In contrast, here it is shown in Chlamydomonas reinhardtii that CO2 depletion under high light levels lowered cellular H2 O2 production, and that elevated CO2 levels increased H2 O2 production. Using various photosynthetic and mitochondrial mutants of C. reinhardtii, the chloroplast was identified as the main source of elevated H2 O2 production under high CO2 availability. High light levels under low CO2 availability induced photoprotective mechanisms called non-photochemical quenching, or NPQ, including state transitions (qT) and high energy state quenching (qE). The qE-deficient mutant npq4 produced more H2 O2 than wild-type cells under high light levels, although less so under high CO2 availability, whereas it demonstrated equal or greater enzymatic H2 O2 -degrading capacity. The qT-deficient mutant stt7-9 produced the same H2 O2 as wild-type cells under high CO2 availability. Physiological levels of H2 O2 were able to hinder qT and the induction of state 2, providing an explanation for why under high light levels and high CO2 availability wild-type cells behaved like stt7-9 cells stuck in state 1. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.

  15. [Hypoallergenic milks (HA formulas) in infant nutrition].

    Science.gov (United States)

    Zoppi, G

    1993-01-01

    According to the definition of the European Scientific Committee for Food, hypoallergenic or hypoantigenic formulas (HA-formulas) are those which contain hydrolysed protein derived both from casein or whey. Soy-based formulas are not comprised in this definition since it has been demonstrated from several years that soy-protein, in several circumstances, may be highly allergenic. Hypoallergenic formulas contain besides hydrolysed protein, carbohydrate and lipid in amount and proportion similar to those indicated by ESPGAN recommendations on adapted formulas. As far as it concerns composition in lipid, recently great attention has been given to optimal supply and ratio of omega 3 and omega 6 fatty acids. Hypoallergenic formulas are therefore suitable for balanced nutrition of suckling infants. Specific indications on prevention of atopic diseases are not treated.

  16. Building highly available control system applications with Advanced Telecom Computing Architecture and open standards

    Science.gov (United States)

    Kazakov, Artem; Furukawa, Kazuro

    2010-11-01

    Requirements for modern and future control systems for large projects like International Linear Collider demand high availability for control system components. Recently telecom industry came up with a great open hardware specification - Advanced Telecom Computing Architecture (ATCA). This specification is aimed for better reliability, availability and serviceability. Since its first market appearance in 2004, ATCA platform has shown tremendous growth and proved to be stable and well represented by a number of vendors. ATCA is an industry standard for highly available systems. On the other hand Service Availability Forum, a consortium of leading communications and computing companies, describes interaction between hardware and software. SAF defines a set of specifications such as Hardware Platform Interface, Application Interface Specification. SAF specifications provide extensive description of highly available systems, services and their interfaces. Originally aimed for telecom applications, these specifications can be used for accelerator controls software as well. This study describes benefits of using these specifications and their possible adoption to accelerator control systems. It is demonstrated how EPICS Redundant IOC was extended using Hardware Platform Interface specification, which made it possible to utilize benefits of the ATCA platform.

  17. Availability and Price of High Quality Day Care and Female Employment

    DEFF Research Database (Denmark)

    Simonsen, Marianne

    In this paper I analyse to what degree availability and price of high quality publicly subsidised childcare affects female employment for women living in couples following maternity leave. The results show that unrestricted access to day care has a significantly positive effct on female employment...

  18. Low nutrient availability reduces high-irradiance–induced viability loss inoceanic phytoplankton

    NARCIS (Netherlands)

    Kulk, G.; van de Poll, W.H.; Visser, R.J.W.; Buma, A.G.J.

    2013-01-01

    In situ viability of oceanic phytoplankton may be relatively low in open oceans. This is assumed to be related to the high-irradiance and low-nutrient conditions typical for oligotrophic regions. However, experimental evidence for this phenomenon was not yet available. In the present study, the impo

  19. A transfer technique for high mobility graphene devices on commercially available hexagonal boron nitride

    NARCIS (Netherlands)

    Zomer, P. J.; Dash, S. P.; Tombros, N.; van Wees, B. J.

    2011-01-01

    We present electronic transport measurements of single and bilayer graphene on commercially available hexagonal boron nitride. We extract mobilities as high as 125 000 cm(2) V-1 s(-1) at room temperature and 275 000 cm(2) V-1 s(-1) at 4.2 K. The excellent quality is supported by the early developmen

  20. High School Physics Availability: Results from the 2012-13 Nationwide Survey of High School Physics Teachers. Focus On

    Science.gov (United States)

    White, Susan; Tesfaye, Casey Langer

    2014-01-01

    In this report, the authors share their analysis of the data from over 3,500 high schools in the U.S. beginning with an examination of the availability of physics in U.S. high schools. The schools in their sample are a nationally-representative random sample of the almost 25,000 high schools in forty-nine of the fifty states. Table 1 shows the…

  1. The HaDREB2 transcription factor enhances basal thermotolerance and longevity of seeds through functional interaction with HaHSFA9

    Directory of Open Access Journals (Sweden)

    Carranco Raúl

    2009-06-01

    Full Text Available Abstract Background Transcription factor HaDREB2 was identified in sunflower (Helianthus annuus L. as a drought-responsive element-binding factor 2 (DREB2 with unique properties. HaDREB2 and the sunflower Heat Shock Factor A9 (HaHSFA9 co-activated the Hahsp17.6G1 promoter in sunflower embryos. Both factors could be involved in transcriptional co-activation of additional small heat stress protein (sHSP promoters, and thus contribute to the HaHSFA9-mediated enhancement of longevity and basal thermotolerance of seeds. Results We found that overexpression of HaDREB2 in seeds did not enhance longevity. This was deduced from assays of basal thermotolerance and controlled seed-deterioration, which were performed with transgenic tobacco. Furthermore, the constitutive overexpression of HaDREB2 did not increase thermotolerance in seedlings or result in the accumulation of HSPs at normal growth temperatures. In contrast, when HaDREB2 and HaHSFA9 were conjointly overexpressed in seeds, we observed positive effects on seed longevity, beyond those observed with overexpression of HaHSFA9 alone. Such additional effects are accompanied by a subtle enhancement of the accumulation of subsets of sHSPs belonging to the CI and CII cytosolic classes. Conclusion Our results reveal the functional interdependency of HaDREB2 and HaHSFA9 in seeds. HaDREB2 differs from other previously characterized DREB2 factors in plants in terms of its unique functional interaction with the seed-specific HaHSFA9 factor. No functional interaction between HaDREB2 and HaHSFA9 was observed when both factors were conjointly overexpressed in vegetative tissues. We therefore suggest that additional, seed-specific factors, or protein modifications, could be required for the functional interaction between HaDREB2 and HaHSFA9.

  2. Adhesive strength of hydroxyl apatite(HA coating and biomechanics behavior of HA-coated prosthesis:an experimental study

    Directory of Open Access Journals (Sweden)

    Tian-yang ZHANG

    2011-05-01

    Full Text Available Objective To explore the influence of adhesive strength of hydroxyapatite(HA coating on the post-implantation stability of HA-coated prosthesis.Methods The adhesive strength and biomechanics behavior of HA coating were studied by histopathological observation,material parameters and biomechanical testing,the titanium(Ti-coated prosthesis was employed as control.Results Scratch test showed that the adhesive strength of HA coating was significantly lower than that of Ti coating(P < 0.01.Histopathological examination and bone morphometry showed that,at the early stage of prosthesis implantation,the bony growth around HA-coated prosthesis was significantly higher than that around Ti-coated prosthesis(P < 0.01,but the ultimate shear strength of HA-coated prosthesis was much lower than that of Ti-coated prosthesis(P < 0.01.After the push-out test with prosthesis,histopathological observation showed that there were accumulations of clump-and strip-like granular residues on the surface of bones that newly grew around the HA-coated prosthesis,and surface energy-dispersive X-ray spectroscopy(EDX analysis also confirmed that the shear stress induced HA decohesion from the substrate of prosthesis.Conclusions Although HA coating showed a satisfactory effect on early bone formation and prosthetic stability,due to the deficiencies of adhesive strength,the early stability of prosthesis may be gradually destroyed by the shear loads of human body and coating degradation.

  3. Designing high availability systems DFSS and classical reliability techniques with practical real life examples

    CERN Document Server

    Taylor, Zachary

    2014-01-01

    A practical, step-by-step guide to designing world-class, high availability systems using both classical and DFSS reliability techniques Whether designing telecom, aerospace, automotive, medical, financial, or public safety systems, every engineer aims for the utmost reliability and availability in the systems he, or she, designs. But between the dream of world-class performance and reality falls the shadow of complexities that can bedevil even the most rigorous design process. While there are an array of robust predictive engineering tools, there has been no single-source guide to understan

  4. Laser treatment of plasma sprayed HA coatings

    NARCIS (Netherlands)

    Khor, KA; Vreeling, A; Dong, ZL; Cheang, P

    1999-01-01

    Laser treatment was conducted on plasma sprayed hydroxyapatite (HA) coatings using a Nd-YAG pulse laser. Various laser parameters were investigated. The results showed that the HA surface melted when an energy level of greater than or equal to 2 J and a spot size of 2 mm was employed during continuo

  5. ha õhtu seltskonnalaud / Kaire Nurk

    Index Scriptorium Estoniae

    Nurk, Kaire, 1960-

    2005-01-01

    ha õhtusöömaaja teemast kunstis. Saksa kunstiajaloolase Horst Schwebeli poolt Leonardo da Vinci "Püha õhtusöömaaja" XX sajandi "töötlustes" välja toodud poliitilise agitatsiooni ja ühisest probleemist haaratud seltskonna kujutiste suunast. Laua kujutisest kunstis

  6. ha õhtu seltskonnalaud / Kaire Nurk

    Index Scriptorium Estoniae

    Nurk, Kaire, 1960-

    2005-01-01

    ha õhtusöömaaja teemast kunstis. Saksa kunstiajaloolase Horst Schwebeli poolt Leonardo da Vinci "Püha õhtusöömaaja" XX sajandi "töötlustes" välja toodud poliitilise agitatsiooni ja ühisest probleemist haaratud seltskonna kujutiste suunast. Laua kujutisest kunstis

  7. Laser treatment of plasma sprayed HA coatings

    NARCIS (Netherlands)

    Khor, KA; Vreeling, A; Dong, ZL; Cheang, P

    1999-01-01

    Laser treatment was conducted on plasma sprayed hydroxyapatite (HA) coatings using a Nd-YAG pulse laser. Various laser parameters were investigated. The results showed that the HA surface melted when an energy level of greater than or equal to 2 J and a spot size of 2 mm was employed during continuo

  8. Implementation and use of a highly available and innovative IaaS solution: the Cloud Area Padovana

    Science.gov (United States)

    Aiftimiei, C.; Andreetto, P.; Bertocco, S.; Biasotto, M.; Dal Pra, S.; Costa, F.; Crescente, A.; Dorigo, A.; Fantinel, S.; Fanzago, F.; Frizziero, E.; Gulmini, M.; Michelotto, M.; Sgaravatto, M.; Traldi, S.; Venaruzzo, M.; Verlato, M.; Zangrando, L.

    2015-12-01

    While in the business world the cloud paradigm is typically implemented purchasing resources and services from third party providers (e.g. Amazon), in the scientific environment there's usually the need of on-premises IaaS infrastructures which allow efficient usage of the hardware distributed among (and owned by) different scientific administrative domains. In addition, the requirement of open source adoption has led to the choice of products like OpenStack by many organizations. We describe a use case of the Italian National Institute for Nuclear Physics (INFN) which resulted in the implementation of a unique cloud service, called ’Cloud Area Padovana’, which encompasses resources spread over two different sites: the INFN Legnaro National Laboratories and the INFN Padova division. We describe how this IaaS has been implemented, which technologies have been adopted and how services have been configured in high-availability (HA) mode. We also discuss how identity and authorization management were implemented, adopting a widely accepted standard architecture based on SAML2 and OpenID: by leveraging the versatility of those standards the integration with authentication federations like IDEM was implemented. We also discuss some other innovative developments, such as a pluggable scheduler, implemented as an extension of the native OpenStack scheduler, which allows the allocation of resources according to a fair-share based model and which provides a persistent queuing mechanism for handling user requests that can not be immediately served. Tools, technologies, procedures used to install, configure, monitor, operate this cloud service are also discussed. Finally we present some examples that show how this IaaS infrastructure is being used.

  9. Availability and Price of High Quality Day Care and Female Employment

    DEFF Research Database (Denmark)

    Simonsen, Marianne

    In this paper I analyse to what degree availability and price of high quality publicly subsidised childcare affects female employment for women living in couples following maternity leave. The results show that unrestricted access to day care has a significantly positive effct on female employmen.......The price effect is significantly negative: An increase in the price of child care of C=1 will decrease the female employment with 0.08% corresponding to a price elasticity of −0.17. This effect prevails during the first 12 months after childbirth.......In this paper I analyse to what degree availability and price of high quality publicly subsidised childcare affects female employment for women living in couples following maternity leave. The results show that unrestricted access to day care has a significantly positive effct on female employment...

  10. Deeper snow alters soil nutrient availability and leaf nutrient status in high Arctic tundra

    DEFF Research Database (Denmark)

    Semenchuk, Philipp R.; Elberling, Bo; Amtorp, Cecilie

    2015-01-01

    Nitrogen (N) mineralization, nutrient availability, and plant growth in the Arctic are often restricted by low temperatures. Predicted increases of cold-season temperatures may be important for plant nutrient availability and growth, given that N mineralization is also taking place during the cold...... season. Changing nutrient availability may be reflected in plant N and chlorophyll content and lead to increased photosynthetic capacity, plant growth, and ultimately carbon (C) assimilation by plants. In this study, we increased snow depth and thereby cold-season soil temperatures in high Arctic...... for some species. Responses to cold-season soil warming are vegetation type- and species-specific, with potentially stronger responses in moister vegetation types. This study therefore highlights the contrasting effect of snow in a tundra landscape and has important implications for projections of whole...

  11. HaVec: An Efficient de Bruijn Graph Construction Algorithm for Genome Assembly

    Directory of Open Access Journals (Sweden)

    Md Mahfuzer Rahman

    2017-01-01

    Full Text Available Background. The rapid advancement of sequencing technologies has made it possible to regularly produce millions of high-quality reads from the DNA samples in the sequencing laboratories. To this end, the de Bruijn graph is a popular data structure in the genome assembly literature for efficient representation and processing of data. Due to the number of nodes in a de Bruijn graph, the main barrier here is the memory and runtime. Therefore, this area has received significant attention in contemporary literature. Results. In this paper, we present an approach called HaVec that attempts to achieve a balance between the memory consumption and the running time. HaVec uses a hash table along with an auxiliary vector data structure to store the de Bruijn graph thereby improving the total memory usage and the running time. A critical and noteworthy feature of HaVec is that it exhibits no false positive error. Conclusions. In general, the graph construction procedure takes the major share of the time involved in an assembly process. HaVec can be seen as a significant advancement in this aspect. We anticipate that HaVec will be extremely useful in the de Bruijn graph-based genome assembly.

  12. Constellation Ground Systems Launch Availability Analysis: Enhancing Highly Reliable Launch Systems Design

    Science.gov (United States)

    Gernand, Jeffrey L.; Gillespie, Amanda M.; Monaghan, Mark W.; Cummings, Nicholas H.

    2010-01-01

    Success of the Constellation Program's lunar architecture requires successfully launching two vehicles, Ares I/Orion and Ares V/Altair, in a very limited time period. The reliability and maintainability of flight vehicles and ground systems must deliver a high probability of successfully launching the second vehicle in order to avoid wasting the on-orbit asset launched by the first vehicle. The Ground Operations Project determined which ground subsystems had the potential to affect the probability of the second launch and allocated quantitative availability requirements to these subsystems. The Ground Operations Project also developed a methodology to estimate subsystem reliability, availability and maintainability to ensure that ground subsystems complied with allocated launch availability and maintainability requirements. The verification analysis developed quantitative estimates of subsystem availability based on design documentation; testing results, and other information. Where appropriate, actual performance history was used for legacy subsystems or comparative components that will support Constellation. The results of the verification analysis will be used to verify compliance with requirements and to highlight design or performance shortcomings for further decision-making. This case study will discuss the subsystem requirements allocation process, describe the ground systems methodology for completing quantitative reliability, availability and maintainability analysis, and present findings and observation based on analysis leading to the Ground Systems Preliminary Design Review milestone.

  13. High availability through full redundancy of the CMS detector controls system

    CERN Document Server

    Bauer, Gerry; Bouffet, Olivier; Bowen, Matthew; Branson, James G; Bukowiec, Sebastian; Ciganek, Marek; Cittolin, Sergio; Jose Antonio Coarasa; Deldicque, Christian; Dobson, Marc; Dupont, Aymeric; Erhan, Samim; Flossdorf, Alexander; Gigi, Dominique; Glege, Frank; Gomez-Reino, Robert; Hartl, Christian; Hegeman, Jeroen; Holzner, André; Yi Ling Hwong; Masetti, Lorenzo; Meijers, Frans; Meschi, Emilio; Mommsen, Remigius K; O'Dell, Vivian; Orsini, Luciano; Paus, Christoph; Petrucci, Andrea; Pieri, Marco; Polese, Giovanni; Racz, Attila; Raginel, Olivier; Sakulin, Hannes; Sani, Matteo; Schwick, Christoph; Shpakov, Dennis; Simon, Michal; Andrei Cristian Spataru; Sumorok, Konstanty

    2012-01-01

    The CMS detector control system (DCS) is responsible for controlling and monitoring the detector status and for the operation of all CMS sub detectors and infrastructure. This is required to ensure safe and efficient data taking so that high quality physics data can be recorded. The current system architecture is composed of more than 100 servers in order to provide the required processing resources. An optimization of the system software and hardware architecture is under development to ensure redundancy of all the controlled sub-systems and to reduce any downtime due to hardware or software failures. The new optimized structure is based mainly on powerful and highly reliable blade servers and makes use of a fully redundant approach, guaranteeing high availability and reliability. The analysis of the requirements, the challenges, the improvements and the optimized system architecture as well as its specific hardware and software solutions are presented.

  14. Unusually high food availability in Kaikoura Canyon linked to distinct deep-sea nematode community

    Science.gov (United States)

    Leduc, D.; Rowden, A. A.; Nodder, S. D.; Berkenbusch, K.; Probert, P. K.; Hadfield, M. G.

    2014-06-01

    Kaikoura Canyon, on the eastern New Zealand continental margin, is the most productive, non-chemosynthetic deep-sea habitat described to date, with megafaunal biomass 100-fold higher than those of other deep-sea habitats. The present study, which focused on free-living nematodes, provides the first comparison of faunal community structure and diversity between Kaikoura Canyon and nearby open slope habitats. Results show substantially higher food availability in the canyon relative to open slope sediments, which probably reflects greater levels of primary productivity above the canyon, coupled with downwelling and/or topographically-induced channelling, which serves to concentrate surface-derived organic matter along the canyon axis. This high food availability appears to be responsible for the elevated nematode biomass in Kaikoura Canyon, with values exceeding all published nematode biomass data from canyons elsewhere. There was also markedly lower local species diversity of nematodes inside the canyon relative to the open slope habitat, as well as a distinct community structure. The canyon community was dominated by species, such as Sabateria pulchra, which were absent from the open slope and are typically associated with highly eutrophic and/or disturbed environments. The presence of these taxa, as well as the low observed diversity, is likely to reflect the high food availability, and potentially the high levels of physically and biologically induced disturbance within the canyon. Kaikoura Canyon is a relatively small habitat characterised by different environmental conditions that makes a disproportionate contribution to deep-sea diversity in the region, despite its low species richness.

  15. The ATLAS Software Installation System v2: a highly available system to install and validate Grid and Cloud sites via Panda

    Science.gov (United States)

    De Salvo, A.; Kataoka, M.; Sanchez Pineda, A.; Smirnov, Y.

    2015-12-01

    The ATLAS Installation System v2 is the evolution of the original system, used since 2003. The original tool has been completely re-designed in terms of database backend and components, adding support for submission to multiple backends, including the original Workload Management Service (WMS) and the new PanDA modules. The database engine has been changed from plain MySQL to Galera/Percona and the table structure has been optimized to allow a full High-Availability (HA) solution over Wide Area Network. The servlets, running on each frontend, have been also decoupled from local settings, to allow an easy scalability of the system, including the possibility of an HA system with multiple sites. The clients can also be run in multiple copies and in different geographical locations, and take care of sending the installation and validation jobs to the target Grid or Cloud sites. Moreover, the Installation Database is used as source of parameters by the automatic agents running in CVMFS, in order to install the software and distribute it to the sites. The system is in production for ATLAS since 2013, having as main sites in HA the INFN Roma Tier 2 and the CERN Agile Infrastructure. The Light Job Submission Framework for Installation (LJSFi) v2 engine is directly interfacing with PanDA for the Job Management, the Atlas Grid Information System (AGIS) for the site parameter configurations, and CVMFS for both core components and the installation of the software itself. LJSFi2 is also able to use other plugins, and is essentially Virtual Organization (VO) agnostic, so can be directly used and extended to cope with the requirements of any Grid or Cloud enabled VO. In this work we will present the architecture, performance, status and possible evolutions to the system for the LHC Run2 and beyond.

  16. Innovative use of controlled availability fertilizers with high performance for intensive agriculture and environmental conservation

    Institute of Scientific and Technical Information of China (English)

    Sadao SHOJI

    2005-01-01

    A variety of slow release fertilizers, controlled release (availability) fertilizers (CAFs),and stability fertilizers have been developed in response to the serious drawbacks of the conventional fertilizers since the early 1960's. Of these fertilizers, CAFs which are coated with resin are consumed in the largest quantity in the world. Selecting CAFs with higher performance, the author will discuss about: 1) Innovation of agro-technologies for various field crops including new concepts of fertilizer application, 2) high yielding of field crops, 3) enhancing quality and safety of farm products, and 4) controlling the adverse effect of intensive agriculture on the environment.

  17. Availability and cost estimate of a high naphthene, modified aviation turbine fuel

    Science.gov (United States)

    Prok, George M.

    1988-01-01

    Information from an Air Force study was used to determine the potential availability and cost of a modified conventional fuel with a naphthene content which could have a thermal stability near that of JP-7 for high-speed civil transports. Results showed sufficient capacity for a fuel made of a blend of 50 percent naphthenic straight run kerosene and 50 percent hydrocracked product, assuming a near-term requirement of 210,000 BBL per day. Fuel cost would be as low as 62.5 to 64.5 cents per gallon, assuming 20 dollars per barrel for crude.

  18. High-frequency nutrient monitoring to infer seasonal patterns in catchment source availability, mobilisation and delivery.

    Science.gov (United States)

    Bende-Michl, Ulrike; Verburg, Kirsten; Cresswell, Hamish P

    2013-11-01

    To explore the value of high-frequency monitoring to characterise and explain riverine nutrient concentration dynamics, total phosphorus (TP), reactive phosphorus (RP), ammonium (NH4-N) and nitrate (NO3-N) concentrations were measured hourly over a 2-year period in the Duck River, in north-western Tasmania, Australia, draining a 369-km(2) mixed land use catchment area. River discharge was observed at the same location and frequency, spanning a wide range of hydrological conditions. Nutrient concentrations changed rapidly and were higher than previously observed. Maximum nutrient concentrations were 2,577 μg L(-1) TP, 1,572 μg L(-1) RP, 972 μg L(-1) NH₄-N and 1,983 μg L(-1) NO₃-N, respectively. Different nutrient response patterns were evident at seasonal, individual event and diurnal time scales-patterns that had gone largely undetected in previous less frequent water quality sampling. Interpretation of these patterns in terms of nutrient source availability, mobilisation and delivery to the stream allowed the development of a conceptual model of catchment nutrient dynamics. Functional stages of nutrient release were identified for the Duck River catchment and were supported by a cluster analysis which confirmed the similarities and differences in nutrient responses caused by the sequence of hydrologic events: (1) a build-up of nutrients during periods with low hydrologic activity, (2) flushing of readily available nutrient sources at the onset of the high flow period, followed by (3) a switch from transport to supply limitation, (4) the accessibility of new nutrient sources with increasing catchment wetness and hydrologic connectivity and (5) high nutrient spikes occurring when new sources become available that are easily mobilised with quickly re-established hydrologic connectivity. Diurnal variations that could be influenced by riverine processes and/or localised point sources were also identified as part of stage (1) and during late recession of some of

  19. Availability of high-magnitude streamflow for groundwater banking in the Central Valley, California

    Science.gov (United States)

    Kocis, Tiffany N.; Dahlke, Helen E.

    2017-08-01

    California’s climate is characterized by the largest precipitation and streamflow variability observed within the conterminous US This, combined with chronic groundwater overdraft of 0.6-3.5 km3 yr-1, creates the need to identify additional surface water sources available for groundwater recharge using methods such as agricultural groundwater banking, aquifer storage and recovery, and spreading basins. High-magnitude streamflow, i.e. flow above the 90th percentile, that exceeds environmental flow requirements and current surface water allocations under California water rights, could be a viable source of surface water for groundwater banking. Here, we present a comprehensive analysis of the magnitude, frequency, duration and timing of high-magnitude streamflow (HMF) for 93 stream gauges covering the Sacramento, San Joaquin and Tulare basins in California. The results show that in an average year with HMF approximately 3.2 km3 of high-magnitude flow is exported from the entire Central Valley to the Sacramento-San Joaquin Delta often at times when environmental flow requirements of the Delta and major rivers are exceeded. High-magnitude flow occurs, on average, during 7 and 4.7 out of 10 years in the Sacramento River and the San Joaquin-Tulare Basins, respectively, from just a few storm events (5-7 1-day peak events) lasting for 25-30 days between November and April. The results suggest that there is sufficient unmanaged surface water physically available to mitigate long-term groundwater overdraft in the Central Valley.

  20. The ATLAS Software Installation System v2: a highly available system to install and validate Grid and Cloud sites via Panda

    CERN Document Server

    De Salvo, Alessandro; The ATLAS collaboration; Smirnov, Yuri; Sanchez, Arturo

    2015-01-01

    The ATLAS Installation System v2 is the evolution of the original system, used since 2003. The original tool has been completely re-designed in terms of database backend and components, adding support for submission to multiple backends, including the original WMS and the new Panda modules. The database engine has been changed from plain MySQL to Galera/Percona and the table structure has been optimized to allow a full High-Availability (HA) solution over WAN. The servlets, running on each frontend, have been also decoupled from local settings, to allow an easy scalability of the system, including the possibility of an HA system with multiple sites. The clients can also be run in multiple copies and in different geographical locations, and take care of sending the installation and validation jobs to the target Grid or Cloud sites. Moreover, the Installation DB is used as source of parameters by the automatic agents running in CVMFS, in order to install the software and distribute it to the sites. The system i...

  1. Sexually transmitted infections treatment and care available to high risk populations in Pakistan.

    Science.gov (United States)

    Rahimtoola, Minal; Hussain, Hamidah; Khowaja, Saira N; Khan, Aamir J

    2008-01-01

    Limited literature exists on the quality and availability of treatment and care of sexually transmitted infections (STIs) in Pakistan. This article aims to document existing services for the care and treatment of STIs available in Pakistan's public and private sectors to high risk groups (HRG), particularly the transgendered population. We conducted a cross-sectional survey to document STI services in Lahore, Karachi, Rawalpindi, Peshawar, and Quetta. Seventy-three interviews were administered with health service providers at the 3 largest public sector hospitals in each city, as well as with general physicians and traditional healers in the private sector. Twenty-five nongovernmental organizations (NGO) providing STI services were also interviewed. Fewer than 45% of private and public sector general practitioners had been trained in STI treatment after the completion of their medical curriculum, and none of the traditional healers had received any formal training or information on STIs. The World Health Organization (WHO) syndromic management guidelines were followed for STI management by 29% of public and private sector doctors and 5% of traditional healers. STI drugs were available at no cost at 44% of NGOs and at some public sector hospitals. Our findings show that although providers do treat HRGs for STIs, there are significant limitations in their ability to provide these services. These deterrents include, but are not limited to, a lack of STI training of service providers, privacy and adherence to recommended WHO syndromic management guidelines, and costly diagnostic and consultation fees.

  2. JOSHUA: Symmetric Active/Active Replication for Highly Available HPC Job and Resource Management

    Energy Technology Data Exchange (ETDEWEB)

    Uhlemann, Kai [ORNL; Engelmann, Christian [ORNL; Scott, Steven L [ORNL

    2006-01-01

    Most of today's HPC systems employ a single head node for control, which represents a single point of failure as it interrupts an entire HPC system upon failure. Furthermore, it is also a single point of control as it disables an entire HPC system until repair. One of the most important HPC system service running on the head node is the job and resource management. If it goes down, all currently running jobs loose the service they report back to. They have to be restarted once the head node is up and running again. With this paper, we present a generic approach for providing symmetric active/active replication for highly available HPC job and resource management. The JOSHUA solution provides a virtually synchronous environment for continuous availability without any interruption of service and without any loss of state. Replication is performed externally via the PBS service interface without the need to modify any service code. Test results as well as a reliability analysis of our proof-of-concept prototype implementation show that continuous availability can be provided by JOSHUA with an acceptable performance trade-off.

  3. Immunomodulatory Effects of Hemagglutinin- (HA- Modified A20 B-Cell Lymphoma Expanded as a Brain Tumor on Adoptively Transferred HA-Specific CD4+ T Cells

    Directory of Open Access Journals (Sweden)

    Valentin P. Shichkin

    2014-01-01

    Full Text Available Previously, the mouse A20 B-cell lymphoma engineered to express hemagglutinin (HA antigen (A20HA was used as a systemic tumor model. In this work, we used the A20HA cells as a brain tumor. HA-specific CD4+ T cells were transferred intravenously in a tail vein 5 days after A20HA intracranial inoculation and analyzed on days 2, 9, and 16 after the adoptive transfer by different methods. The transferred cells demonstrated state of activation as early as day 2 after the adoptive transfer and most the of viable HA-specific cells became anergic on day 16. Additionally, symptoms of systemic immunosuppression were observed in mice with massive brain tumors at a late stage of the brain tumor progression (days 20–24 after the A20HA inoculation. Despite that, a deal of HA-specific CD4+ T cells kept the functional activity even at the late stage of A20HA tumor growth. The activated HA-specific CD4+ T cells were found also in the brain of brain-tumor-bearing mice. These cells were still responding to reactivation with HA-peptide in vitro. Our data support an idea about sufficient role of both the tumor-specific and -nonspecific mechanisms inducing immunosuppression in cancer patients.

  4. Understanding the undelaying mechanism of HA-subtyping in the level of physic-chemical characteristics of protein.

    Directory of Open Access Journals (Sweden)

    Mansour Ebrahimi

    Full Text Available The evolution of the influenza A virus to increase its host range is a major concern worldwide. Molecular mechanisms of increasing host range are largely unknown. Influenza surface proteins play determining roles in reorganization of host-sialic acid receptors and host range. In an attempt to uncover the physic-chemical attributes which govern HA subtyping, we performed a large scale functional analysis of over 7000 sequences of 16 different HA subtypes. Large number (896 of physic-chemical protein characteristics were calculated for each HA sequence. Then, 10 different attribute weighting algorithms were used to find the key characteristics distinguishing HA subtypes. Furthermore, to discover machine leaning models which can predict HA subtypes, various Decision Tree, Support Vector Machine, Naïve Bayes, and Neural Network models were trained on calculated protein characteristics dataset as well as 10 trimmed datasets generated by attribute weighting algorithms. The prediction accuracies of the machine learning methods were evaluated by 10-fold cross validation. The results highlighted the frequency of Gln (selected by 80% of attribute weighting algorithms, percentage/frequency of Tyr, percentage of Cys, and frequencies of Try and Glu (selected by 70% of attribute weighting algorithms as the key features that are associated with HA subtyping. Random Forest tree induction algorithm and RBF kernel function of SVM (scaled by grid search showed high accuracy of 98% in clustering and predicting HA subtypes based on protein attributes. Decision tree models were successful in monitoring the short mutation/reassortment paths by which influenza virus can gain the key protein structure of another HA subtype and increase its host range in a short period of time with less energy consumption. Extracting and mining a large number of amino acid attributes of HA subtypes of influenza A virus through supervised algorithms represent a new avenue for

  5. Preparation and properties of HA coating hydrothermally synthesized from plasma sprayed CaHPO4 coating

    Institute of Scientific and Technical Information of China (English)

    FU Tao; HAN Yong; ZHANG Yu-mei; XU Ke-wei

    2001-01-01

    @@ INTRODUCTION Hydroxyapatite (HA) biocoatings can form osseointegration at a shorter time than metallic implants, and plasma sprayed (PS) HA coating has received the widest studies and is now used clinically. However, due to the high temperature of plasma flame, soluble impurity phases and amorphous calcium phosphate were contained which declined the bonding strength of the coating, and spoiled the excellent biological properties of HA.

  6. High sensitivity of broadleaf trees to water availability in northeastern United States

    Science.gov (United States)

    Levesque, Mathieu; Andreu-Hayles, Laia; Pederson, Neil

    2016-04-01

    Broadleaf dominated forests of eastern US cover more than one million km2 and provide ecosystem services to millions of people. High species diversity and a varied sensitivity to drought make it uncertain whether these forests will be carbon sinks or sources under climate change. Ongoing climate change, increased in atmospheric CO2 concentration (ca) and strong reductions in acidic depositions are expected to alter growth and gas exchange of trees, and ultimately forest productivity. Still, the magnitude of these effects is unclear. A better comprehension of the species-specific responses to environmental changes will better inform models and managers on the vulnerability and resiliency of these forests. Here, we combined tree-ring width data with δ13C and δ18O measurements to investigate growth and physiological responses of red oak (Quercus rubra L.) and tulip poplar (Liriodendron tulipifera L.) in northeastern US to changes in water availability, ca and acidic depositions for the period 1950-2014. Based on structural equation modeling approaches, we found that summer water availability (June-August) is the main environmental variable driving growth, water-use efficiency and δ18O of broadleaf trees whereas ca and acidic depositions have little effects. This high sensitivity to moisture availability was also supported by the very strong correlations found between summer vapor pressure deficit (VPD) and tree-ring δ13C (r = 0.67 and 0.71), and δ18O series (r = 0.62 and 0.72), for red oak and tulip poplar, respectively. In contrast, tree-ring width was less sensitive to summer VPD (r = -0.44 and-0.31). Since the mid 1980s, pluvial conditions occurring in northeastern US have increased stomatal conductance, carbon uptake, and growth of both species. Further, the strong spatial field correlations found between the tree-ring δ13C and δ18O and summer VPD indicate a greater sensitivity of eastern US broadleaf forests to moisture availability than previously known

  7. Experience and Lessons learnt from running high availability databases on Network Attached Storage

    CERN Document Server

    Guijarro, Juan Manuel; Segura Chinchilla, Nilo

    2008-01-01

    The Database and Engineering Services Group of CERN's Information Technology Department provides the Oracle based Central Data Base services used in many activities at CERN. In order to provide High Availability and ease management for those services, a NAS (Network Attached Storage) based infrastructure has been set up. It runs several instances of the Oracle RAC (Real Application Cluster) using NFS as share disk space for RAC purposes and Data hosting. It is composed of two private LAN's to provide access to the NAS file servers and Oracle RAC interconnect, both using network bonding. NAS nodes are configured in partnership to prevent having single points of failure and to provide automatic NAS fail-over. This presentation describes that infrastructure and gives some advice on how to automate its management and setup using a Fabric Management framework such as Quattor. It also covers aspects related with NAS Performance and Monitoring as well Data Backup and Archive of such facility using already existing i...

  8. Experience and Lessons learnt from running High Availability Databases on Network Attached Storage

    CERN Document Server

    Guijarro, Manuel

    2008-01-01

    The Database and Engineering Services Group of CERN's Information Technology Department supplies the Oracle Central Database services used in many activities at CERN. In order to provide High Availability and ease management for those services, a NAS (Network Attached Storage) based infrastructure has been setup. It runs several instances of the Oracle RAC (Real Application Cluster) using NFS (Network File System) as shared disk space for RAC purposes and Data hosting. It is composed of two private LANs (Local Area Network), one to provide access to the NAS filers and a second to implement the Oracle RAC private interconnect, both using Network Bonding. NAS filers are configured in partnership to prevent having single points of failure and to provide automatic NAS filer fail-over.

  9. Low-Cost Solutions Using the Infrastructure as a Service with High Availability and Virtualization Model

    Directory of Open Access Journals (Sweden)

    Cesar Armando Moreira Zambrano

    2017-02-01

    Full Text Available This paper presents the results obtained from the implementation of an infrastructure to improve technological services of email, virtual learning environment, digital repository and virtual library at the Polytechnic Agricultural Higher School of Manabí (Polytechnic School of Agriculture of Manabí, ESPAM, through the use of high availability and virtualization mechanisms to provide more reliable resources. Virtualization is an empowering and cutting-edge technology that is transforming the operation of technological services, but it involves a paradigm shift in serviceoriented information technologies and cloud computing. To execute each of the processes the V-cycle methodology was used as a strategy. Virtualization services empowers companies and institutions by transforming how they operate to be at the forefront of innovation in their services as a technological solution. So the implementation of redundant technology in the ESPAM, has allowed its technological services are always operative, for the benefit of the university community, because if there were failures in the main system or services, the backups will be enabled quickly allowing the systems come into operation immediately.

  10. Implementasi Cloud Storage Menggunakan OwnCloud yang High-Availability

    Directory of Open Access Journals (Sweden)

    Ikhwan Ar-Razy

    2016-04-01

    Full Text Available Implementation of practicum courses in Department of Computer Engineering Diponegoro University has some drawbacks, one of them is a lot of lab assistant and the practitioner experiencing difficulties in terms of archiving. One solution to solve the problem is implementing a shared file storage system that is easy and can be accessed by both practitioners or lab assistants. The purpose of this research is to build a cloud-based storage systems that are reliable to preventing crash damage hardware and high availability. The purpose of this research is achieved by designing the appropriate methodology. The result of this research is a storage system that is on the server side by using virtualization and data replication (DRBD as a storage method. The system is composed of two physical servers and one virtual server. Physical servers are using Proxmox VE as operating system and virtual server is using Ubuntu Server as operating system. OwnCloud applications and files are stored in the virtual server. File storage system has several major functions, which are: upload, download, user management, remove, and restore. The functions are executed through web pages, desktop application and Android application.

  11. A software and hardware architecture for a high-availability PACS.

    Science.gov (United States)

    Gutiérrez-Martínez, Josefina; Núñez-Gaona, Marco Antonio; Aguirre-Meneses, Heriberto; Delgado-Esquerra, Ruth Evelin

    2012-08-01

    Increasing radiology studies has led to the emergence of new requirements for management medical information, mainly affecting the storage of digital images. Today, it is a necessary interaction between workflow management and legal rules that govern it, to allow an efficient control of medical technology and associated costs. Another important topic that is growing in importance within the healthcare sector is compliance, which includes the retention of studies, information security, and patient privacy. Previously, we conducted a series of extensive analysis and measurements of pre-existing operating conditions. These studies and projects have been described in other papers. The first phase: hardware and software installation and initial tests were completed in March 2006. The storage phase was built step by step until the PACS-INR was totally completed. Two important aspects were considered in the integration of components: (1) the reliability and performance of the system to transfer and display DICOM images, and (2) the availability of data backups for disaster recovery and downtime scenarios. This paper describes the high-availability model for a large-scale PACS to support the storage and retrieve of data using CAS and DAS technologies to provide an open storage platform. This solution offers a simple framework that integrates and automates the information at low cost and minimum risk. Likewise, the model allows an optimized use of the information infrastructure in the clinical environment. The tests of the model include massive data migration, openness, scalability, and standard compatibility to avoid locking data into a proprietary technology.

  12. Synergistic interaction of platelet derived growth factor (PDGF) with the surface of PLLA/Col/HA and PLLA/HA scaffolds produces rapid osteogenic differentiation.

    Science.gov (United States)

    Raghavendran, Hanumantha Rao Balaji; Mohan, Saktiswaren; Genasan, Krishnamurithy; Murali, Malliga Raman; Naveen, Sangeetha Vasudevaraj; Talebian, Sepehr; McKean, Robert; Kamarul, Tunku

    2016-03-01

    Scaffolds with structural features similar to the extracellular matrix stimulate rapid osteogenic differentiation in favorable microenvironment and with growth factor supplementation. In this study, the osteogenic potential of electrospun poly-l-lactide/hydroxyapatite/collagen (PLLA/Col/HA, PLLA/HA and PLLA/Col) scaffolds were tested in vitro with the supplementation of platelet derived growth factor-BB (PDGF-BB). Cell attachment and topography, mineralization, extracellular matrix protein localization, and gene expression of the human mesenchymal stromal cells were compared between the fibrous scaffolds PLLA/Col/HA, PLLA/Col, and PLLA/HA. The levels of osteocalcin, calcium, and mineralization were significantly greater in the PLLA/Col/HA and PLLA/HA compared with PLLA/Col. High expression of fibronectin, intracellular adhesion molecule, cadherin, and collagen 1 (Col1) suggests that PLLA/Col/HA and PLLA/HA scaffolds had superior osteoinductivity than PLLA/Col. Additionally, osteopontin, osteocalcin, osterix, Runt-related transcription factor 2 (Runx2), and bone morphogenic protein (BMP2) expression were higher in PLLA/Col/HA and PLLA/HA compared with PLLA/Col. In comparison with PLLA/Col, the PLLA/Col/HA and PLLA/HA scaffolds presented a significant upregulation of the genes Runx2, Col 1, Integrin, osteonectin (ON), bone gamma-carboxyglutamic acid-containing protein (BGALP), osteopontin (OPN), and BMP2. The upregulation of these genes was further increased with PDGF-BB supplementation. These results show that PDGF-BB acts synergistically with PLLA/Col/HA and PLLA/HA to enhance the osteogenic differentiation potential. Therefore, this combination can be used for the rapid expansion of bone marrow stromal cells into bone-forming cells for tissue engineering.

  13. Effect of Water-Glass Coating on HA and HA-TCP Samples for MSCs Adhesion, Proliferation, and Differentiation

    Directory of Open Access Journals (Sweden)

    Indu Bajpai

    2016-01-01

    Full Text Available Ca-P and silicon based materials have become very popular as bone tissue engineering materials. In this study, water-glass (also known as sodium silicate glass was coated on sintered hydroxyapatite (HA and HA-TCP (TCP stands for tricalcium phosphate samples and subsequently heat-treated at 600°C for 2 hrs. X-rays diffraction showed the presence of β- and α-TCP phases along with HA in the HA-TCP samples. Samples without coating, with water-glass coating, and heat-treated after water-glass coating were used to observe the adhesion and proliferation response of bone marrow derived-mesenchymal stem cells (MSCs. Cell culture was carried out for 4 hrs, 1 day, and 7 days. Interestingly, all samples showed similar response for cell adhesion and proliferation up to 7-day culture but fibronectin, E-cadherin, and osteogenic differentiation related genes (osteocalcin and osteopontin were significantly induced in heat-treated water-glass coated HA-TCP samples. A water-glass coating on Ca-P samples was not found to influence the cell proliferation response significantly but activated some extracellular matrix genes and induced osteogenic differentiation in the MSCs.

  14. ArcticDEM; A Publically Available, High Resolution Elevation Model of the Arctic

    Science.gov (United States)

    Morin, Paul; Porter, Claire; Cloutier, Michael; Howat, Ian; Noh, Myoung-Jong; Willis, Michael; Bates, Brian; Willamson, Cathleen; Peterman, Kennith

    2016-04-01

    A Digital Elevation Model (DEM) of the Arctic is needed for a large number of reasons, including: measuring and understanding rapid, ongoing changes to the Arctic landscape resulting from climate change and human use and mitigation and adaptation planning for Arctic communities. The topography of the Arctic is more poorly mapped than most other regions of Earth due to logistical costs and the limits of satellite missions with low-latitude inclinations. A convergence of civilian, high-quality sub-meter stereo imagery; petascale computing and open source photogrammetry software has made it possible to produce a complete, very high resolution (2 to 8-meter posting), elevation model of the Arctic. A partnership between the US National Geospatial-intelligence Agency and a team led by the US National Science Foundation funded Polar Geospatial Center is using stereo imagery from DigitalGlobe's Worldview-1, 2 and 3 satellites and the Ohio State University's Surface Extraction with TIN-based Search-space Minimization (SETSM) software running on the University of Illinois's Blue Water supercomputer to address this challenge. The final product will be a seemless, 2-m posting digital surface model mosaic of the entire Arctic above 60 North including all of Alaska, Greenland and Kamchatka. We will also make available the more than 300,000 individual time-stamped DSM strip pairs that were used to assemble the mosaic. The Arctic DEM will have a vertical precision of better than 0.5m and can be used to examine changes in land surfaces such as those caused by permafrost degradation or the evolution of arctic rivers and floodplains. The data set can also be used to highlight changing geomorphology due to Earth surface mass transport processes occurring in active volcanic and glacial environments. When complete the ArcticDEM will catapult the Arctic from the worst to among the best mapped regions on Earth.

  15. Computational Environments and Analysis methods available on the NCI High Performance Computing (HPC) and High Performance Data (HPD) Platform

    Science.gov (United States)

    Evans, B. J. K.; Foster, C.; Minchin, S. A.; Pugh, T.; Lewis, A.; Wyborn, L. A.; Evans, B. J.; Uhlherr, A.

    2014-12-01

    The National Computational Infrastructure (NCI) has established a powerful in-situ computational environment to enable both high performance computing and data-intensive science across a wide spectrum of national environmental data collections - in particular climate, observational data and geoscientific assets. This paper examines 1) the computational environments that supports the modelling and data processing pipelines, 2) the analysis environments and methods to support data analysis, and 3) the progress in addressing harmonisation of the underlying data collections for future transdisciplinary research that enable accurate climate projections. NCI makes available 10+ PB major data collections from both the government and research sectors based on six themes: 1) weather, climate, and earth system science model simulations, 2) marine and earth observations, 3) geosciences, 4) terrestrial ecosystems, 5) water and hydrology, and 6) astronomy, social and biosciences. Collectively they span the lithosphere, crust, biosphere, hydrosphere, troposphere, and stratosphere. The data is largely sourced from NCI's partners (which include the custodians of many of the national scientific records), major research communities, and collaborating overseas organisations. The data is accessible within an integrated HPC-HPD environment - a 1.2 PFlop supercomputer (Raijin), a HPC class 3000 core OpenStack cloud system and several highly connected large scale and high-bandwidth Lustre filesystems. This computational environment supports a catalogue of integrated reusable software and workflows from earth system and ecosystem modelling, weather research, satellite and other observed data processing and analysis. To enable transdisciplinary research on this scale, data needs to be harmonised so that researchers can readily apply techniques and software across the corpus of data available and not be constrained to work within artificial disciplinary boundaries. Future challenges will

  16. Central noradrenaline transporter availability in highly obese, non-depressed individuals

    Energy Technology Data Exchange (ETDEWEB)

    Hesse, Swen; Sabri, Osama [University of Leipzig, Department of Nuclear Medicine, Leipzig (Germany); Leipzig University Medical Centre, Integrated Treatment and Research Centre (IFB) Adiposity Diseases, Leipzig (Germany); Becker, Georg-Alexander; Bresch, Anke; Luthardt, Julia; Patt, Marianne; Meyer, Philipp M. [University of Leipzig, Department of Nuclear Medicine, Leipzig (Germany); Rullmann, Michael [University of Leipzig, Department of Nuclear Medicine, Leipzig (Germany); Leipzig University Medical Centre, Integrated Treatment and Research Centre (IFB) Adiposity Diseases, Leipzig (Germany); Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig (Germany); Hankir, Mohammed K.; Zientek, Franziska; Reissig, Georg; Fenske, Wiebke K. [Leipzig University Medical Centre, Integrated Treatment and Research Centre (IFB) Adiposity Diseases, Leipzig (Germany); Arelin, Katrin [Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig (Germany); University of Leipzig, Day Clinic for Cognitive Neurology, Leipzig (Germany); Lobsien, Donald [University of Leipzig, Department of Neuroradiology, Leipzig (Germany); Mueller, Ulrich [University of Cambridge, Department of Psychiatry and Behavioural and Clinical Neuroscience Institute, Cambridge (United Kingdom); Baldofski, S.; Hilbert, Anja [Leipzig University Medical Centre, Integrated Treatment and Research Centre (IFB) Adiposity Diseases, Leipzig (Germany); University of Leipzig, Department of Medical Psychology and Medical Sociology, Leipzig (Germany); Blueher, Matthias [University of Leipzig, Department of Internal Medicine, Leipzig (Germany); Fasshauer, Mathias; Stumvoll, Michael [Leipzig University Medical Centre, Integrated Treatment and Research Centre (IFB) Adiposity Diseases, Leipzig (Germany); University of Leipzig, Department of Internal Medicine, Leipzig (Germany); Ding, Yu-Shin [New York University School of Medicine, Departments of Radiology and Psychiatry, New York, NY (United States)

    2017-06-15

    The brain noradrenaline (NA) system plays an important role in the central nervous control of energy balance and is thus implicated in the pathogenesis of obesity. The specific processes modulated by this neurotransmitter which lead to obesity and overeating are still a matter of debate. We tested the hypothesis that in vivo NA transporter (NAT) availability is changed in obesity by using positron emission tomography (PET) and S,S-[{sup 11}C]O-methylreboxetine (MRB) in twenty subjects comprising ten highly obese (body mass index BMI > 35 kg/m{sup 2}), metabolically healthy, non-depressed individuals and ten non-obese (BMI < 30 kg/m{sup 2}) healthy controls. Overall, we found no significant differences in binding potential (BP{sub ND}) values between obese and non-obese individuals in the investigated brain regions, including the NAT-rich thalamus (0.40 ± 0.14 vs. 0.41 ± 0.18; p = 0.84) though additional discriminant analysis correctly identified individual group affiliation based on regional BP{sub ND} in all but one (control) case. Furthermore, inter-regional correlation analyses indicated different BP{sub ND} patterns between both groups but this did not survive testing for multiple comparions. Our data do not find an overall involvement of NAT changes in human obesity. However, preliminary secondary findings of distinct regional and associative patterns warrant further investigation. (orig.)

  17. Assessment of commercially available ion exchange materials for cesium removal from highly alkaline wastes

    Energy Technology Data Exchange (ETDEWEB)

    Brooks, K.P.; Kim, A.Y.; Kurath, D.E.

    1996-04-01

    Approximately 61 million gallons of nuclear waste generated in plutonium production, radionuclide removal campaigns, and research and development activities is stored on the Department of Energy`s Hanford Site, near Richland, Washington. Although the pretreatment process and disposal requirements are still being defined, most pretreatment scenarios include removal of cesium from the aqueous streams. In many cases, after cesium is removed, the dissolved salt cakes and supernates can be disposed of as LLW. Ion exchange has been a leading candidate for this separation. Ion exchange systems have the advantage of simplicity of equipment and operation and provide many theoretical stages in a small space. The organic ion exchange material Duolite{trademark} CS-100 has been selected as the baseline exchanger for conceptual design of the Initial Pretreatment Module (IPM). Use of CS-100 was chosen because it is considered a conservative, technologically feasible approach. During FY 96, final resin down-selection will occur for IPM Title 1 design. Alternate ion exchange materials for cesium exchange will be considered at that time. The purpose of this report is to conduct a search for commercially available ion exchange materials which could potentially replace CS-100. This report will provide where possible a comparison of these resin in their ability to remove low concentrations of cesium from highly alkaline solutions. Materials which show promise can be studied further, while less encouraging resins can be eliminated from consideration.

  18. A prototype Infrastructure for Cloud-based distributed services in High Availability over WAN

    Science.gov (United States)

    Bulfon, C.; Carlino, G.; De Salvo, A.; Doria, A.; Graziosi, C.; Pardi, S.; Sanchez, A.; Carboni, M.; Bolletta, P.; Puccio, L.; Capone, V.; Merola, L.

    2015-12-01

    In this work we present the architectural and performance studies concerning a prototype of a distributed Tier2 infrastructure for HEP, instantiated between the two Italian sites of INFN-Romal and INFN-Napoli. The network infrastructure is based on a Layer-2 geographical link, provided by the Italian NREN (GARR), directly connecting the two remote LANs of the named sites. By exploiting the possibilities offered by the new distributed file systems, a shared storage area with synchronous copy has been set up. The computing infrastructure, based on an OpenStack facility, is using a set of distributed Hypervisors installed in both sites. The main parameter to be taken into account when managing two remote sites with a single framework is the effect of the latency, due to the distance and the end-to-end service overhead. In order to understand the capabilities and limits of our setup, the impact of latency has been investigated by means of a set of stress tests, including data I/O throughput, metadata access performance evaluation and network occupancy, during the life cycle of a Virtual Machine. A set of resilience tests has also been performed, in order to verify the stability of the system on the event of hardware or software faults. The results of this work show that the reliability and robustness of the chosen architecture are effective enough to build a production system and to provide common services. This prototype can also be extended to multiple sites with small changes of the network topology, thus creating a National Network of Cloud-based distributed services, in HA over WAN.

  19. HA03 as an Iranian Candidate Concealed Antigen for Vaccination against Hyalomma anatolicum anatolicum: Comparative Structural and In silico Studies

    Directory of Open Access Journals (Sweden)

    Mohammadi, A.

    2013-12-01

    Full Text Available In the last decades researchers had focused on developing a vaccine against tick based on protective antigen. Recombinant vaccines based on concealed antigen from Boophilus microplus have been developed in Australia and Cuba by the name of TICKGARD and GAVAC (De La Fuente and Kocan, 2006. Further studies on this antigen have shown some extent of protection against other species (De Vos et al., 2001. In Iran most important species is Hyalomma anatolicum and limited information about its control are available. This paper reports structural and polymorphic analysis of HA03 as an Iranian candidate concealed antigen of H. a. anatolicum deposited in Gen-Bank .(Aghaeipour et al. GQ228820. The comparison between this antigen and other mid gut concealed antigen that their characteristics are available in GenBank showed there are high rate of similarity between them. The HA03 amino acid sequence had a homology of around 89%, 64%, 56% with HA98, BM86, BM95 respectively. Potential of MHC class I and II binding region indicated a considerable variation between BM86 antigen and its efficiency against Iranian H. a. anatolicum. In addition, predicted major of hydrophobisity and similarity in N-glycosylation besides large amount of cystein and seven EGF like regions presented in protein structure revealed that value of HA03 as a new protective antigen and the necessity of the development, BM86 homolog of H. a. anatolicum HA03 based recombinant vaccine.

  20. High-resolution prediction of soil available water content within the crop root zone

    Science.gov (United States)

    Haghverdi, Amir; Leib, Brian G.; Washington-Allen, Robert A.; Ayers, Paul D.; Buschermohle, Michael J.

    2015-11-01

    A detailed understanding of soil hydraulic properties, particularly soil available water content (AWC) within the effective root zone, is needed to optimally schedule irrigation in fields with substantial spatial heterogeneity. However, it is difficult and time consuming to directly measure soil hydraulic properties. Therefore, easily collected and measured soil properties, such as soil texture and/or bulk density, that are well correlated with hydraulic properties are used as proxies to develop pedotransfer functions (PTF). In this study, multiple modeling scenarios were developed and evaluated to indirectly predict high resolution AWC maps within the effective root zone. The modeling techniques included kriging, co-kriging, regression kriging, artificial neural networks (NN) and geographically weighted regression (GWR). The efficiency of soil apparent electrical conductivity (ECa) as proximal data in the modeling process was assessed. There was a good agreement (root mean square error (RMSE) = 0.052 cm3 cm-3 and r = 0.88) between observed and point prediction of water contents using pseudo continuous PTFs. We found that both GWR (mean RMSE = 0.062 cm3 cm-3) and regression kriging (mean RMSE = 0.063 cm3 cm-3) produced the best water content maps with these accuracies improved up to 19% when ECa was used as an ancillary soil attribute in the interpolation process. The maps indicated fourfold differences in AWC between coarse- and fine-textured soils across the study site. This provided a template for future investigations for evaluating the efficiency of variable rate irrigation management scenarios in accounting for the spatial heterogeneity of soil hydraulic attributes.

  1. Effects of bound versus soluble pentosan polysulphate in PEG/HA-based hydrogels tailored for intervertebral disc regeneration.

    Science.gov (United States)

    Frith, Jessica E; Menzies, Donna J; Cameron, Andrew R; Ghosh, P; Whitehead, Darryl L; Gronthos, S; Zannettino, Andrew C W; Cooper-White, Justin J

    2014-01-01

    Previous reports in the literature investigating chondrogenesis in mesenchymal progenitor cell (MPC) cultures have confirmed the chondro-inductive potential of pentosan polysulphate (PPS), a highly sulphated semi-synthetic polysaccharide, when added as a soluble component to culture media under standard aggregate-assay conditions or to poly(ethylene glycol)/hyaluronic acid (PEG/HA)-based hydrogels, even in the absence of inductive factors (e.g. TGFβ). In this present study, we aimed to assess whether a 'bound' PPS would have greater activity and availability over a soluble PPS, as a media additive or when incorporated into PEG/HA-based hydrogels. We achieved this by covalently pre-binding the PPS to the HA component of the gel (forming a new molecule, HA-PPS). We firstly investigated the activity of HA-PPS compared to free PPS, when added as a soluble factor to culture media. Cell proliferation, as determined by CCK8 and EdU assay, was decreased in the presence of either bound or free PPS whilst chondrogenic differentiation, as determined by DMMB assay and histology, was enhanced. In all cases, the effect of the bound PPS (HA-PPS) was more potent than that of the unbound form. These results alone suggest wider applications for this new molecule, either as a culture supplement or as a coating for scaffolds targeted at chondrogenic differentiation or maturation. We then investigated the incorporation of HA-PPS into a PEG/HA-based hydrogel system, by simply substituting some of the HA for HA-PPS. Rheological testing confirmed that incorporation of either HA-PPS or PPS did not significantly affect gelation kinetics, final hydrogel modulus or degradation rate but had a small, but significant, effect on swelling. When encapsulated in the hydrogels, MPCs retained good viability and rapidly adopted a rounded morphology. Histological analysis of both GAG and collagen deposition after 21 days showed that the incorporation of the bound-PPS into the hydrogel resulted in

  2. Systematic review of available evidence on 11 high-priced inpatient orphan drugs

    NARCIS (Netherlands)

    T.A. Kanters (Tim A.); C. de Sonneville (Caroline); W.K. Redekop (Ken); L. van Hakkaart-van Roijen (Leona)

    2013-01-01

    markdownabstract__Abstract__ __Background__: Attention for Evidence Based Medicine (EBM) is growing, but evidence for orphan drugs is argued to be limited and inferior. This study systematically reviews the available evidence on clinical effectiveness, costeffectiveness and budget impact for orph

  3. A comparative study on in vitro osteogenic priming potential of electron spun scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for tissue engineering application.

    Science.gov (United States)

    Balaji Raghavendran, Hanumantha Rao; Puvaneswary, Subramaniam; Talebian, Sepehr; Murali, Malliga R; Raman Murali, Malliga; Naveen, Sangeetha V; Vasudevaraj Naveen, Sangeetha; Krishnamurithy, G; McKean, Robert; Kamarul, Tunku

    2014-01-01

    A comparative study on the in vitro osteogenic potential of electrospun poly-L-lactide/hydroxyapatite/collagen (PLLA/HA/Col, PLLA/HA, and PLLA/Col) scaffolds was conducted. The morphology, chemical composition, and surface roughness of the fibrous scaffolds were examined. Furthermore, cell attachment, distribution, morphology, mineralization, extracellular matrix protein localization, and gene expression of human mesenchymal stromal cells (hMSCs) differentiated on the fibrous scaffolds PLLA/Col/HA, PLLA/Col, and PLLA/HA were also analyzed. The electrospun scaffolds with a diameter of 200-950 nm demonstrated well-formed interconnected fibrous network structure, which supported the growth of hMSCs. When compared with PLLA/H%A and PLLA/Col scaffolds, PLLA/Col/HA scaffolds presented a higher density of viable cells and significant upregulation of genes associated with osteogenic lineage, which were achieved without the use of specific medium or growth factors. These results were supported by the elevated levels of calcium, osteocalcin, and mineralization (PCol/HA scaffolds exhibited superior osteoinductivity, when compared with PLLA/Col or PLLA/HA scaffolds. These findings indicated that the fibrous structure and synergistic action of Col and nano-HA with high-molecular-weight PLLA played a vital role in inducing osteogenic differentiation of hMSCs. The data obtained in this study demonstrated that the developed fibrous PLLA/Col/HA biocomposite scaffold may be supportive for stem cell based therapies for bone repair, when compared with the other two scaffolds.

  4. RELIABILITY, AVAILABILITY, AND SERVICEABILITY FOR PETASCALE HIGH-END COMPUTING AND BEYOND

    Energy Technology Data Exchange (ETDEWEB)

    Chokchai " Box" Leangsuksun

    2011-05-31

    Our project is a multi-institutional research effort that adopts interplay of RELIABILITY, AVAILABILITY, and SERVICEABILITY (RAS) aspects for solving resilience issues in highend scientific computing in the next generation of supercomputers. results lie in the following tracks: Failure prediction in a large scale HPC; Investigate reliability issues and mitigation techniques including in GPGPU-based HPC system; HPC resilience runtime & tools.

  5. RF/optical shared aperture for high availability wideband communication RF/FSO links

    Energy Technology Data Exchange (ETDEWEB)

    Ruggiero, Anthony J; Pao, Hsueh-yuan; Sargis, Paul

    2014-04-29

    An RF/Optical shared aperture is capable of transmitting and receiving optical signals and RF signals simultaneously. This technology enables compact wide bandwidth communications systems with 100% availability in clear air turbulence, rain and fog. The functions of an optical telescope and an RF reflector antenna are combined into a single compact package by installing an RF feed at either of the focal points of a modified Gregorian telescope.

  6. RF/optical shared aperture for high availability wideband communication RF/FSO links

    Energy Technology Data Exchange (ETDEWEB)

    Ruggiero, Anthony J; Pao, Hsueh-yuan; Sargis, Paul

    2015-03-24

    An RF/Optical shared aperture is capable of transmitting and receiving optical signals and RF signals simultaneously. This technology enables compact wide bandwidth communications systems with 100% availability in clear air turbulence, rain and fog. The functions of an optical telescope and an RF reflector antenna are combined into a single compact package by installing an RF feed at either of the focal points of a modified Gregorian telescope.

  7. Investigating Commercially Available Technology for Language Learners in Higher Education within the High Functioning Disability Spectrum

    Science.gov (United States)

    Savvidou, Georgia; Loizides, Fernando

    2016-01-01

    This work presents the assistive use of a combination of technologies in language learning to individuals with high functioning disabilities within a higher education environment. The primary aim of this research is to introduce the initial findings of a pilot exploratory user test which aims to facilitate a better understanding of the suitability…

  8. Building a highly available and intrusion tolerant database security and protection system (DSPS)

    Institute of Scientific and Technical Information of China (English)

    蔡亮; 杨小虎; 董金祥

    2003-01-01

    Database Security and Protection System (DSPS) is a security platform for fighting malicious DBMS. The security and performance are critical to DSPS. The authors suggested a key management scheme by combining the server group structure to improve availability and the key distribution structure needed by proactive security. This paper detailed the implementation of proactive security in DSPS. After thorough performance analysis, the authors concluded that the performance difference between the replicated mechanism and proactive mechanism becomes smaller and smaller with increasing number of concurrent connections; and that proactive security is very useful and practical for large, critical applications.

  9. Building a highly available and intrusion tolerant database security and protection system ( DSPS)

    Institute of Scientific and Technical Information of China (English)

    蔡亮; 杨小虎; 董金祥

    2003-01-01

    Database Security and Protection System (DSPS) is a security platform for fighting malicious DBMS. The security and performance are critical to DSPS. The authors suggested a key management scheme by combining the server group structure to improve availability and the key distribution structure needed by proactive security. This paper detailed the implementation of proactive security in DSPS. After thorough performane analysis, the authors concluded that the performance difference between the replicated mechanism and proactive mechanism becomes smaller and smaller with increasing number of concurrent connections ; and that proactive security is very useful and practical for large, critical applications.

  10. HDPE-HA composites synthetized by in situ polymerization with different filler content

    Science.gov (United States)

    Hermán, V.; Karam, A.; Albano, C.; Romero, K.; González, G.

    2012-07-01

    In Situ ethylene polymerization was used to synthesize high density polyethylene - hydroxyapatite (HDPE-HA) composites, employing Cp2ZrCl2/MAO as catalytic system. A good dispersion of HA into the HDPE matrix was obtained when the following synthesis conditions were combined: high stirring velocities (2000 rpm), low quantities of solvent (100 mL), and 10 °C. Under these conditions different filler content was used to synthetized HDPE-HA composites. An interaction between HA and HDPE was obtained by FTIR. On the other hand, thermal analysis indicated that no significant differences were observed between HDPE and the composites.

  11. Metal availability in a highly contaminated, dredged-sediment disposal site: Field measurements and geochemical modeling

    Energy Technology Data Exchange (ETDEWEB)

    Lions, Julie, E-mail: j.lions@brgm.f [BRGM, 3 Avenue Claude Guillemin, 45060 Orleans Cedex 2 (France); Centre National de Recherche sur les Sites et Sols Pollues, BP 537, 59505 Douai cedex (France); Guerin, Valerie; Bataillard, Philippe [BRGM, 3 Avenue Claude Guillemin, 45060 Orleans Cedex 2 (France); Centre National de Recherche sur les Sites et Sols Pollues, BP 537, 59505 Douai cedex (France); Lee, Jan van der [Mines ParisTech, Centre de Geosciences, 77305 Fontainebleau Cedex (France); Laboudigue, Agnes [Univ Lille Nord de France, F-59000 Lille (France); EMDouai, MPE-GCE, F-59500 Douai (France); Centre National de Recherche sur les Sites et Sols Pollues, BP 537, 59505 Douai cedex (France)

    2010-09-15

    Two complementary approaches were used to characterize arsenic and metal mobilizations from a dredged-sediment disposal site: a detailed field study combined with hydrogeochemical modeling. Contaminants in sediments were found to be mainly present as sulfides subject to oxidation. Secondary phases (carbonates, sulfates, (hydr)oxides) were also observed. Oxidative processes occurred at different rates depending on physicochemical conditions and contaminant contents in the sediment. Two distinct areas were identified on the site, each corresponding to a specific contaminant mobility behavior. In a reducing area, Fe and As were highly soluble and illustrated anoxic behavior. In well-oxygenated material, groundwater was highly contaminated in Zn, Cd and Pb. A third zone in which sediments and groundwater were less contaminated was also characterized. This study enabled us to prioritize remediation work, which should aim to limit infiltration and long-term environmental impact. - A detailed case study of metal behavior in a dredged-sediment disposal site combined with geochemical modeling.

  12. Daily training with high carbohydrate availability increases exogenous carbohydrate oxidation during endurance cycling.

    Science.gov (United States)

    Cox, Gregory R; Clark, Sally A; Cox, Amanda J; Halson, Shona L; Hargreaves, Mark; Hawley, John A; Jeacocke, Nikki; Snow, Rodney J; Yeo, Wee Kian; Burke, Louise M

    2010-07-01

    We determined the effects of varying daily carbohydrate intake by providing or withholding carbohydrate during daily training on endurance performance, whole body rates of substrate oxidation, and selected mitochondrial enzymes. Sixteen endurance-trained cyclists or triathletes were pair matched and randomly allocated to either a high-carbohydrate group (High group; n = 8) or an energy-matched low-carbohydrate group (Low group; n = 8) for 28 days. Immediately before study commencement and during the final 5 days, subjects undertook a 5-day test block in which they completed an exercise trial consisting of a 100 min of steady-state cycling (100SS) followed by a 7-kJ/kg time trial on two occasions separated by 72 h. In a counterbalanced design, subjects consumed either water (water trial) or a 10% glucose solution (glucose trial) throughout the exercise trial. A muscle biopsy was taken from the vastus lateralis muscle on day 1 of the first test block, and rates of substrate oxidation were determined throughout 100SS. Training induced a marked increase in maximal citrate synthase activity after the intervention in the High group (27 vs. 34 micromol x g(-1) x min(-1), P < 0.001). Tracer-derived estimates of exogenous glucose oxidation during 100SS in the glucose trial increased from 54.6 to 63.6 g (P < 0.01) in the High group with no change in the Low group. Cycling performance improved by approximately 6% after training. We conclude that altering total daily carbohydrate intake by providing or withholding carbohydrate during daily training in trained athletes results in differences in selected metabolic adaptations to exercise, including the oxidation of exogenous carbohydrate. However, these metabolic changes do not alter the training-induced magnitude of increase in exercise performance.

  13. Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H

    Directory of Open Access Journals (Sweden)

    Guorui Yao

    2017-03-01

    Full Text Available Botulinum neurotoxins (BoNTs, which have been exploited as cosmetics and muscle-disorder treatment medicines for decades, are well known for their extreme neurotoxicity to humans. They pose a potential bioterrorism threat because they cause botulism, a flaccid muscular paralysis-associated disease that requires immediate antitoxin treatment and intensive care over a long period of time. In addition to the existing seven established BoNT serotypes (BoNT/A–G, a new mosaic toxin type termed BoNT/HA (aka type FA or H was reported recently. Sequence analyses indicate that the receptor-binding domain (HC of BoNT/HA is ~84% identical to that of BoNT/A1. However, BoNT/HA responds differently to some potent BoNT/A-neutralizing antibodies (e.g., CR2 that target the HC. Therefore, it raises a serious concern as to whether BoNT/HA poses a new threat to our biosecurity. In this study, we report the first high-resolution crystal structure of BoNT/HA-HC at 1.8 Å. Sequence and structure analyses reveal that BoNT/HA and BoNT/A1 are different regarding their binding to cell-surface receptors including both polysialoganglioside (PSG and synaptic vesicle glycoprotein 2 (SV2. Furthermore, the new structure also provides explanations for the ~540-fold decreased affinity of antibody CR2 towards BoNT/HA compared to BoNT/A1. Taken together, these new findings advance our understanding of the structure and function of this newly identified toxin at the molecular level, and pave the way for the future development of more effective countermeasures.

  14. A study on improving mechanical properties of porous HA tissue engineering scaffolds by hot isostatic pressing

    Energy Technology Data Exchange (ETDEWEB)

    Zhao Jing [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China); Xiao Suguang [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China); Lu Xiong [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China); Wang Jianxin [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China); Weng Jie [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China)

    2006-12-15

    Various interconnected porous hydroxyapatite (HA) ceramic scaffolds are universally used to induct the tissue growth for bone repair and replacement, and serve to support the adhesion, transfer, proliferation and differentiation of cells. Impregnation of polyurethane sponges with a ceramic slurry is adopted to produce highly porous HA ceramic scaffolds with a 3D interconnected structure. However, high porosity always accompanies a decrease in the strength of the HA ceramic scaffolds. Therefore, it is significant to improve the strength of the HA ceramic scaffolds with highly interconnected porosity so that they are more suitable in clinical applications. In this work, highly porous HA ceramic scaffolds are first produced by the polymer impregnation approach, and subsequently further sintered by hot isostatic pressing (HIP). The phase composition, macro- and micro-porous structure, sintering and mechanical properties of the porous HA scaffolds are investigated by x-ray diffraction (XRD), scanning electron microscopy (SEM), nanoindentation analysis and compressive test. The experimental results show that the nanohardness and compressive strength of HIP-sintered porous HA ceramics are higher than those of commonly sintered HA scaffolds. The HIP technique can effectively improve the sintering property and densification of porous HA ceramic scaffolds, so inducing an increase in the compression strength.

  15. Future water availability in North African dams simulated by high-resolution regional climate models

    Science.gov (United States)

    Tramblay, Yves; Jarlan, Lionel; Hanich, Lahoucine; Somot, Samuel

    2016-04-01

    In North Africa, the countries of Morocco, Algeria and Tunisia are already experiencing water scarcity and a strong interannual variability of precipitation. To better manage their existing water resources, several dams and reservoirs have been built on most large river catchments. The objective of this study is to provide quantitative scenarios of future changes in water availability for the 47 major dams and reservoirs catchments located in North Africa. An ensemble of regional climate models (RCM) with a spatial resolution of 12km, driven by different general circulation models (GCM), from the EuroCORDEX experiment have been considered to analyze the projected changes on temperature, precipitation and potential evapotranspiration (PET) for two scenarios (RCP4.5 and RCP8.5) and two time horizons (2040-2065 and 2065-2090). PET is estimated from RCM outputs either with the FAO-Penman-Monteith (PM) equation, requiring air temperature, relative humidity, net radiation and wind, or with the Hargreave Samani (HS) equation, requiring only air temperature. The water balance is analyzed by comparing the climatic demand and supply of water, considering that for most of these catchments groundwater storage is negligible over long time periods. Results indicated a future temperature increase for all catchments between +1.8° and +4.2°, depending on the emission scenario and the time period considered. Precipitation is projected to decrease between -14% to -27%, mainly in winter and spring, with a strong East to West gradient. PET computed from PM or HS formulas provided very similar estimates and projections, ranging between +7% to +18%. Changes in PET are mostly driven by rising temperatures and are greatest during dry summer months than for the wet winter season. Therefore the increased PET has a lower impact than declining precipitation on future water availability, which is expected to decrease by -19% to -33% on average.

  16. In Vitro Degradation of PHBV Scaffolds and nHA/PHBV Composite Scaffolds Containing Hydroxyapatite Nanoparticles for Bone Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Naznin Sultana

    2012-01-01

    Full Text Available This paper investigated the long-term in vitro degradation properties of scaffolds based on biodegradable polymers and osteoconductive bioceramic/polymer composite materials for the application of bone tissue engineering. The three-dimensional porous scaffolds were fabricated using emulsion-freezing/freeze-drying technique using poly(hydroxybutyrate-co-hydroxyvalerate (PHBV which is a natural biodegradable and biocompatible polymer. Nanosized hydroxyapatite (nHA particles were successfully incorporated into the PHBV scaffolds to render the scaffolds osteoconductive. The PHBV and nHA/PHBV scaffolds were systematically evaluated using various techniques in terms of mechanical strength, porosity, porous morphology, and in vitro degradation. PHBV and nHA/PHBV scaffolds degraded over time in phosphate-buffered saline at 37°C. PHBV polymer scaffolds exhibited slow molecular weight loss and weight loss in the in vitro physiological environment. Accelerated weight loss was observed in nHA incorporated PHBV composite scaffolds. An increasing trend of crystallinity was observed during the initial period of degradation time. The compressive properties decreased more than 40% after 5-month in vitro degradation. Together with interconnected pores, high porosity, suitable mechanical properties, and slow degradation profile obtained from long-term degradation studies, the PHBV scaffolds and osteoconductive nHA/PHBV composite scaffolds showed promises for bone tissue engineering application.

  17. Fish bones--a highly available calcium source for growing pigs.

    Science.gov (United States)

    Malde, M K; Graff, I E; Siljander-Rasi, H; Venäläinen, E; Julshamn, K; Pedersen, J I; Valaja, J

    2010-10-01

    In general, there is a lack of scientific documentation of nutritional value of marine by-products. The bone fraction from fish has been regarded as waste. Due to the high mineral content of fish bones, this material can be well suitable as a natural calcium source. In the present study, apparent calcium absorption of different fish bone sources was tested using growing pigs. The experimental diets consisted of boiled salmon frames, or salmon, saithe or cod bones treated with enzymes. Calcium carbonate (CaCO(3)) was used as control. The experimental diets were formulated to contain 0.7% total calcium of which the added calcium source to be tested contributed about 71% (study 1) and 86% (study 2). Except for the calcium and phosphorus sources, the animals received similar basal diets. Apparent calcium digestibility coefficient was calculated using yttrium as indicator (both studies) and was based on complete collection of faeces and urine (study 2). The experimental design was parallel and cross-over in study 1 and study 2, respectively. In study 1, piglets getting salmon bone treated with enzymes had significantly higher calcium absorption than piglets getting boiled fish bone or calcium carbonate. Therefore, in the second study only enzymatically treated fish bones were included. The higher calcium absorption from enzymatically treated salmon bone was also found in study 2, but this time not significant. Calcium from boiled salmon bones in study I, and from enzymatically treated saithe and cod bones in study II were absorbed as well as the calcium carbonate control. The results indicate that fish bones may be a useful and well absorbed calcium source. Due to the high mineral content of the bone fraction, salmon bones can be well suitable as a natural calcium and phosphorus source in, for example, food, feed or as supplement.

  18. Cuando la "puesta en el juego" es un hijo que aún no ha nacido.

    Directory of Open Access Journals (Sweden)

    Giuliana Prata

    1990-01-01

    Full Text Available Los juegos de la pareja pueden influir en sus "puestas" un hijo que no ha nacido o que ni siquiera ha sido concebido. Síntomas tales como esterilidad, abortos espontáneos o muertes del niño durante el parto son examinados en este artículo a la luz de esta hipótesis.

  19. Response of Streptococcus suis to iron-restricted growth conditions at high and low oxygen availability.

    Science.gov (United States)

    Winterhoff, Nora; Goethe, Ralph; Gruening, Petra; Valentin-Weigand, Peter

    2004-01-01

    Streptococcus suis (S. suis) is an important pathogen in pigs and has to overcome strict iron limitations in its host environment. Here, we studied iron-restricted growth of a highly virulent S. suis strain in vitro at aerobic and CO2-enriched growth conditions. At both conditions, depleting of iron in the culture medium with nitrilotriacetic acid (NTA) resulted in decreased growth rates and down regulation of several proteins. Sensitivity to NTA was significantly higher at aerobic versus CO2-enriched conditions. Growth could not be restored by addition of host iron sources such as ferritin, hemin, hemoglobin, lactoferrin or transferrin. Accordingly, S. suis was not able to produce detectable amounts of siderophores. On the other hand, growth at iron-restricted conditions was fully restored by addition of Mn2+ (at aerobic and CO2-enriched conditions) or Mg2+ (only at CO2-enriched conditions). In conclusion our results suggest that, unlike many other bacteria, S. suis adapts to iron restricted conditions by a change in its metabolism in order to replace Fe2+ by Mn2+ or Mg2+ rather than by expressing specific iron uptake systems.

  20. Using DNA metabarcoding to investigate honey bee foraging reveals limited flower use despite high floral availability.

    Science.gov (United States)

    de Vere, Natasha; Jones, Laura E; Gilmore, Tegan; Moscrop, Jake; Lowe, Abigail; Smith, Dan; Hegarty, Matthew J; Creer, Simon; Ford, Col R

    2017-02-16

    Understanding which flowers honey bees (Apis mellifera) use for forage can help us to provide suitable plants for healthy honey bee colonies. Accordingly, honey DNA metabarcoding provides a valuable tool for investigating pollen and nectar collection. We investigated early season (April and May) floral choice by honey bees provided with a very high diversity of flowering plants within the National Botanic Garden of Wales. There was a close correspondence between the phenology of flowering and the detection of plants within the honey. Within the study area there were 437 genera of plants in flower during April and May, but only 11% of these were used. Thirty-nine plant taxa were recorded from three hives but only ten at greater than 1%. All three colonies used the same core set of native or near-native plants, typically found in hedgerows and woodlands. The major plants were supplemented with a range of horticultural species, with more variation in plant choice between the honey bee colonies. We conclude that during the spring, honey bees need access to native hedgerows and woodlands to provide major plants for foraging. Gardens provide supplementary flowers that may increase the nutritional diversity of the honey bee diet.

  1. Detection of high-risk human papillomavirus infection in tonsillar specimens using 2 commercially available assays.

    Science.gov (United States)

    Cockerill, Cara C; Orvidas, Laura J; Moore, Eric J; Binnicker, Matthew J; Duresko, Brian J; Espy, Mark J; Cockerill, Franklin R; Tombers, Nicole M; Pritt, Bobbi S

    2016-12-01

    THE OBJECTIVE OF THE STUDY IS TO DETERMINE THE PREVALENCE OF HIGH-RISK HUMAN PAPILLOMAVIRUS (HRHPV) INFECTION IN TONSILLAR SWABS AND TISSUE: Patients undergoing tonsillectomy for nonmalignant causes were enrolled. A flocked swab and fresh tissue were collected from the left and right tonsil of each patient. Specimens were tested for hrHPV DNA using the Roche cobas test and for the presence of E6/E7 messenger RNA using the Hologic Aptima hrHPV test. Of the 193 patients enrolled, 129 were in the pediatric group (ages 1-12years; median, 5years), and 64 were in the adult group (ages 13-55; median, 22years). All swab and tissue specimens were negative for hrHPV by both methods. Positive, negative, and internal controls performed as expected. We found a 0% rate of infection indicating that detectable hrHPV infection in tonsillar tissue appears to be uncommon in the children and adults in the population sampled. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. Using DNA metabarcoding to investigate honey bee foraging reveals limited flower use despite high floral availability

    Science.gov (United States)

    de Vere, Natasha; Jones, Laura E.; Gilmore, Tegan; Moscrop, Jake; Lowe, Abigail; Smith, Dan; Hegarty, Matthew J.; Creer, Simon; Ford, Col R.

    2017-01-01

    Understanding which flowers honey bees (Apis mellifera) use for forage can help us to provide suitable plants for healthy honey bee colonies. Accordingly, honey DNA metabarcoding provides a valuable tool for investigating pollen and nectar collection. We investigated early season (April and May) floral choice by honey bees provided with a very high diversity of flowering plants within the National Botanic Garden of Wales. There was a close correspondence between the phenology of flowering and the detection of plants within the honey. Within the study area there were 437 genera of plants in flower during April and May, but only 11% of these were used. Thirty-nine plant taxa were recorded from three hives but only ten at greater than 1%. All three colonies used the same core set of native or near-native plants, typically found in hedgerows and woodlands. The major plants were supplemented with a range of horticultural species, with more variation in plant choice between the honey bee colonies. We conclude that during the spring, honey bees need access to native hedgerows and woodlands to provide major plants for foraging. Gardens provide supplementary flowers that may increase the nutritional diversity of the honey bee diet. PMID:28205632

  3. El griot no ha muerto, viva el hip-hop

    Directory of Open Access Journals (Sweden)

    Fco. Javier González García-Mamely

    2015-11-01

    Full Text Available La oratura en África ha jugado un papel vital a lo largo de los milenios como un (único medio de preservar y transmitir la historia, la cultura y el imaginario de cada comunidad. El griot es un claro modelo de las artes orales, pero ha sufrido cambios drásticos en su modo de vida y en los recursos narrativos de los que dispone. Tras el mecenazgo de los grandes reyes y las sucesivas llegadas del islam, el colonialismo y los procesos de modernización y occidentalización, los griots se han tenido que adaptar a nuevos patrones, audiencias y medios de comunicación, convirtiéndose en políticos o en figuras del espectáculo. La aparición del rap y el hip-hop reclamando ser los “modernos griots” ha ocasionado una respuesta crítica y una atracción viral hacia el nuevo fenómeno, sea éste un nuevo género o una reinvención de la oratura del antiguo griot.

  4. Preparation and characterization of bio-composite PEEK/nHA

    Science.gov (United States)

    Jin, Y. S.; Bian, C. C.; Zhang, Z. Q.; Zhao, Y.; Yang, L.

    2017-01-01

    PEEK/nHA composite material, with excellent mechanical property as polyetheretherketone (PEEK) and biological activity as hydroxyapatite (HA), has attracted wide attention of medical experts and materials science experts. The addition of hydroxyapatite was the decisive factor for biological activity in PEEK/nHA composite. In this paper, acicular nanohydroxyapatite was prepared by chemical precipitation method with Ca(NO3)2, (NH4)2HPO4 as raw material; PEEK/nHA composite was prepared by solution blending and vacuum sintering method. The composite was characterized with FT-IR, XRD, DSC, TG and mechanical property test. Results showed that the composite has good thermal stability and compressive property when the mass ratio of PEEK to nHA is 10:3; and high nHA content can improve the biological activity of the composite, which can meet the basic requirements for bone tissue engineering scaffold.

  5. Preparation and characterization of stainless steel 316L/HA biocomposite

    Directory of Open Access Journals (Sweden)

    Gilbert Silva

    2013-04-01

    Full Text Available The austenitic stainless steel 316L is the most used metallic biomaterials in orthopedics applications, especially in the manufacture of articulated prostheses and as structural elements in fracture fixation, since it has high mechanical strength. However, because it is biologically inactive, it does not form chemical bond with bone tissue, it is fixed only by morphology. The development of biocomposites of stainless steel with a bioactive material, such as hydroxyapatite - HA, is presented as an alternative to improve the response in the tissue-implant interface. However significant reductions in mechanical properties of the biocomposite can occur. Different compositions of the biocomposite stainless steel 316L/HA (5, 20 and 50 wt. (% HA were prepared by mechanical alloying. After milling the powders for 10 hours, the different compositions of the biocomposite were compacted isostatically and sintered at 1200 ºC for 2 hours. The mechanical properties of the biocomposites were analyzed by compression tests. The powders and the sintered composites were analyzed by scanning electron microscopy (SEM and X-ray diffraction (XRD.

  6. High School Physics Availability: Results from the 2008-09 Nationwide Survey of High School Physics Teachers. Focus On

    Science.gov (United States)

    White, Susan; Tesfaye, Casey Langer

    2010-01-01

    In the fall of 2008, the authors contacted a representative sample of over 3,600 high schools in the U.S.--both public and private--to determine whether or not physics was taught there. They received responses from over 99% of the schools. For the schools which indicated they were offering physics, they obtained contact information for the…

  7. Cassandra high availability

    CERN Document Server

    Strickland, Robbie

    2014-01-01

    If you are a developer or DevOps engineer who understands the basics of Cassandra and are ready to take your knowledge to the next level, then this book is for you. An understanding of the essentials of Cassandra is needed.

  8. Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines

    Science.gov (United States)

    Khurana, Surender; Larkin, Christopher; Verma, Swati; Joshi, Manju B.; Fontana, Juan; Steven, Alasdair C.; King, Lisa R.; Manischewitz, Jody; McCormick, William; Gupta, Rajesh K.; Golding, Hana

    2011-01-01

    Vaccine production and initiation of mass vaccination is a key factor in rapid response to new influenza pandemic. During the 2009–2010 H1N1 pandemic, several bottlenecks were identified, including the delayed availability of vaccine potency reagents. Currently, antisera for the single-radial immunodiffusion (SRID) potency assay are generated in sheep immunized repeatedly with HA released and purified after bromelain-treatment of influenza virus grown in eggs. This approach was a major bottleneck for pandemic H1N1 (H1N1pdm09) potency reagent development in 2009. Alternative approaches are needed to make HA immunogens for generation of SRID reagents in the shortest possible time. In this study, we found that properly folded recombinant HA1 globular domain (rHA1) from several type A viruses including H1N1pdm09 and two H5N1 viruses could be produced efficiently by using a bacterial expression system and subsequent purification. The rHA1 proteins were shown to form functional oligomers of trimers, similar to virus derived HA, and elicited high titer of neutralizing antibodies in rabbits and sheep. Importantly, the immune sera formed precipitation rings with reference antigens in the SRID assay in a dose-dependent manner. The HA contents in multiple H1N1 vaccine products from different manufacturers (and in several lots) as determined with the rHA1-generated sheep sera were similar to the values obtained with a traditionally generated sheep serum from NIBSC. We conclude that bacterially-expressed recombinant HA1 proteins can be produced rapidly and used to generate SRID potency reagents shortly after new influenza strains with pandemic potential are identified. PMID:21704111

  9. Single-dose monomeric HA subunit vaccine generates full protection from influenza challenge

    CSIR Research Space (South Africa)

    Mallajosyula, JK

    2014-03-01

    Full Text Available Recombinant subunit vaccines are an efficient strategy to meet the demands of a possible influenza pandemic, because of rapid and scalable production. However, vaccines made from recombinant hemagglutinin (HA) subunit protein are often of low...

  10. Antibiotic-containing hyaluronic acid gel as an antibacterial carrier: Usefulness of sponge and film-formed HA gel in deep infection.

    Science.gov (United States)

    Matsuno, Hiroaki; Yudoh, Kazuo; Hashimoto, Masamichi; Himeda, Yasukazu; Miyoshi, Teruzo; Yoshida, Kaoru; Kano, Syogo

    2006-03-01

    We have developed a novel bioabsorbable antibacterial carrier using hyaluronic acid (HA) gel for prevention and treatment of orthopedic infections. In this study, we investigated the in vivo antibacterial effects of two forms of this new material, an HA gel sponge and an HA gel film. A titanium cylinder was inserted into the intramedullary cavity of each rabbit femur, along with an HA gel sponge or HA gel film containing antibiotics. The HA gel sponge contained gentamycin, vancomycin, tobramycin, or minomycin. The HA gel film contained gentamycin or vancomycin. After 0, 7, and 14 days, the rabbit bone marrow was collected, and the antibacterial activity of the HA gel was determined by agar diffusion test. As a control, we used Septocoll, a commercially available antibacterial carrier. Both the HA gel sponge and HA gel film exhibited antibacterial activity. The present results indicate that HA gel containing antibiotics is a clinically useful bioabsorbable antibacterial carrier.

  11. Laser Annealing for Gas-Dynamical Spraying of HA Coating upon a Titanium Surface

    Directory of Open Access Journals (Sweden)

    Victor Saphronov

    2015-10-01

    Full Text Available Laser post-heating computer controlled detonation spraying (CCDS and cold spray (CS hybrid processes were proposed for fabrication of near sub micron structure coatings of hydroxyapatite (HA + Ti system. Optical and SEM with energy dispersive X-ray analysis and comparative XRD phase analysis were used to evaluate microstructure. After those hybrid processes, no substantial variation in HA composition was noted by structural and phase examination. Nano-sized HA powders can be recommended for laser annealing CS (LaCS process. Regimes of laser treatment optimal for increasing the adhesion between the HA and titanium coatings, providing more strength, ductility and decreasing of HA destruction in the coatings were determined.

  12. Reconstruction of HaSNPV with helicoverpa hormone receptor 3

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In order to develop a more efficient virus for controlling the cotton bollworm Helicoverpa armigera,Helicoverpa hormone receptor 3 (HHR3), which is involved in the ecdysteroid regulatory pathway, was used to genetically modify wild HaSNPV. HaSNPV-HHR3 budded virus and occlusion body virus were constructed in three steps: preparation of pFastBacHaPhpP10-HHR3 donor plasmid, transposition of HHR3 into the HaBacHZ8 bacmid, and transfection of HzAM1 cells to get HaSNPV-HHR3 virus. HHR3was proved to be expressed in the HaSNPV-HHR3 virus infected HzAM1 cells by immunoblotting. Results of bioassay indicated that the body weight of the HaSNPV-HHR3 infected larvae was lower than the larvae infected with wild virus and uninfected normal larvae, which suggests that HaSNPV-HHR3 delayed larval growth.

  13. An Exploration of Support Factors Available to Higher Education Students with High Functioning Autism or Asperger Syndrome

    Science.gov (United States)

    Rutherford, Emily N.

    2013-01-01

    This qualitative phenomenological research study used narrative inquiry to explore the support factors available to students with High Functioning Autism or Asperger Syndrome in higher education that contribute to their success as perceived by the students. Creswell's (2009) six step method for analyzing phenomenological studies was used to…

  14. An Exploration of Support Factors Available to Higher Education Students with High Functioning Autism or Asperger Syndrome

    Science.gov (United States)

    Rutherford, Emily N.

    2013-01-01

    This qualitative phenomenological research study used narrative inquiry to explore the support factors available to students with High Functioning Autism or Asperger Syndrome in higher education that contribute to their success as perceived by the students. Creswell's (2009) six step method for analyzing phenomenological studies was used to…

  15. The Availability and Utilization of School Library Resources in Some Selected Secondary Schools (High School) in Rivers State

    Science.gov (United States)

    Owate, C. N.; Iroha, Okpa

    2013-01-01

    This study investigates the availability and utilization of school library resources by Secondary School (High School) Students. Eight Selected Secondary Schools in Rivers State, Nigeria were chosen based on their performance in external examinations and geographic locations. In carrying out the research, questionnaires were administered to both…

  16. Study on the genotoxicity of HA/ZrO{sub 2} composite particles in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Quan, Renfu, E-mail: quanrenfu8@yahoo.com [Xiaoshan Traditional Chinese Medical Hospital, ZhengJiang Province 311200 (China); Tang, Yanghua; Huang, Zhongming; Xu, Jinwei [Xiaoshan Traditional Chinese Medical Hospital, ZhengJiang Province 311200 (China); Wu, Xiaochen [School of Materials Science and Engineering, Shanghai University, Shanghai (China); Yang, Disheng [Department of Orthopedics, the second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009 (China)

    2013-04-01

    To evaluate the genotoxicity of the HA/ZrO{sub 2} composite particles by using the micronucleus test (MNT) in vitro. HA/ZrO{sub 2} composite particles prepared by sintering at high temperature and pressure, that used powder of HA and ZrO{sub 2} of different proportions, were compared with pure HA particles and pure ZrO{sub 2} particles. The effect of the composite particles on cell proliferation of rabbit mesenchymal stem cells, and its the genotoxicity to rabbit mesenchymal stem cells were detected by MNT method. The MTT test showed that both pure HA particles and composite particles which contained HA promoted cell proliferation of rabbit mesenchymal stem cells, while pure ZrO{sub 2} particles did not, and there was a significant difference (P < 0.05). The MNT test showed no significant difference between the HA group and the negative control group (P > 0.05), but a significant difference between the HA group and the positive control group (P < 0.05). The difference between the ZrO{sub 2} group and the negative control group was significant (P < 0.01), while the difference between the ZrO{sub 2} group and the positive control group was insignificant (P > 0.05). The genotoxicity of the HA/ZrO{sub 2} composite particle increased with a higher proportion of ZrO{sub 2} and an increase in the concentration of the composite, and the 30 wt.% HA/70% ZrO{sub 2} composite with 200 μg/mL concentration showed significant genotoxicity (P < 0.01). - Highlights: ► HA/ZrO{sub 2} composite particles were prepared by sintering used powder of HA and ZrO{sub 2} of different proportions. ► Evaluate the genotoxicity of the HA/ZrO{sub 2} composite particle in vitro micronucleus test (MNT). ► The genotoxicity of the HA/ZrO{sub 2} composite particle increased with an increase in the proportion of ZrO{sub 2}. ► The 30%wtHA/70% ZrO{sub 2} composite with 200 μg/mL concentration showed significant genotoxicity.

  17. 一种提升高速铁路无线通信网络 HA RQ 性能的方法%Method for Improving HARQ Performance of Mobile Communication System of High-speed Railway

    Institute of Scientific and Technical Information of China (English)

    李莎莎; 方旭明; 罗万团

    2014-01-01

    In the case that the LTE(Long Term Evolution) wireless communication system is applied to high-speed railways , high moving speeds and complicated railway line environments impose great influence un-favourable for HARQ (Hybrid Automatic Retransmission Request ) performance . In this paper , the HARQ performance in high-speed railway environments was analyzed .It shows that the system with HARQ technolo-gy has lower block error rate and higher throughput as compared to the case without it . However , the HARQ performance in high-speed environments is not as good as that in the scenario of public networks where most of the users are with low-speeds . And then , a novel HARQ scheme was proposed , which could enhance the HARQ performance in the overlapping region . In this scheme , the number of maximum retransmission is re-duced w hen the train arrives at a predefined location , and the packets , w hich suffer transmission error or can not be acknowledged before handover triggering ,will be saved by the system ,and retransmitted by the target eNodeB(after the train accesses it) ,and then combined and decoded by the onboard UE(User Equipment) . Performance analysis show s as follow s :T he error rate decreases by 55% w hen these packets are retransmitted in the target cell ;compared to the situation with the fixed number of maximum retransmission , the proposed scheme provides more opportunities for new packets to be transmitted before handover , thus the throughput increases by about 12% when target eNodeB retransmits those forward packets .%在长期演进LTE无线通信系统应用到高速铁路环境时,较高的移动速度、复杂的沿线环境将给系统混合自动重传请求HARQ性能带来较大的不利影响。本文对LTEHARQ在高速铁路场景下的性能进行分析,结果表明,相对于不采用HARQ技术,带有HARQ机制的系统具有较低的误块率、较高的吞吐量;然而,相比于公网静止用户场景,高速铁路移

  18. Preparation and Properties of Biphasic Calcium Phosphate Scaffolds Multiply Coated with HA/PLLA Nanocomposites for Bone Tissue Engineering Applications

    Directory of Open Access Journals (Sweden)

    Lei Nie

    2012-01-01

    Full Text Available A well-developed BCP scaffolds coated with multilayer of HA/PLLA nanocomposites with interconnectivity, high porosity, and moderate compressive strength as well as good biocompatibility were fabricated for bone tissue engineering. After being multiply coated with HA/PLLA nanocomposites, the scaffolds maintained the BCP framework structure, and the porous network structure of scaffolds remained unchanged; however, the compressive strength was increased with the increase of coating layer number of HA/PLLA nanocomposites. The prepared scaffolds showed lower variation of pH values in SBF solution, and an increase of coating layer number led to the decrease of the biodegradation rate at different days. Moreover, the multilayer coating scaffolds had good cytocompatibility, showing no negative effects on cells growth and proliferation. Furthermore, the bone-like apatite layer was built obviously in the interface of scaffold after 21 days after implantation in SD rat muscle. In conclusion, the BCP scaffold coated with multilayer of HA/PLLA nanocomposites could be a candidate as an excellent substitute for damaged or defect bone in bone tissue engineering.

  19. L'oggetto del desiderio ha il colore del vuoto

    Directory of Open Access Journals (Sweden)

    Giovanni Bottiroli

    2011-07-01

    Full Text Available L’oggetto del desiderio non è semplicemente un oggetto empirico; su ciò, con qualche esitazione, potrebbe esistere un consenso generale. Resta la difficoltà di indicare le caratteristiche dell’oggetto del desiderio: che non sono banalmente delle proprietà, e neanche solo delle parti (in senso empirico, come si potrebbe nominarle da un punto di vista mereologico. L’oggetto del desiderio ha uno statuto modale: è un oggetto modalmente diviso, nei registri e nei regimi. Nella dimensione del desiderio, tanto il soggetto quanto l’oggetto sono abitati dal conflitto tra il diviso e l’indiviso. La spinta verso l’indiviso corrisponde a ciò che Freud ha introdotto con la nozione enigmatica di «pulsione di morte». In termini lacaniani, è l’attrazione verso la Cosa (das Ding. E la Cosa vuole coincidere con se stessa, abolendo ogni distinzione. L’oggetto del desiderio non è un dato o una forma empirica, per attraente che sia: l’attrazione che esercita proviene dal caos. Alla pericolosa coincidenza del «vuoto-pieno» (das Ding il desiderio oppone il vuoto logico della non-coincidenza. Una teoria del desiderio non può fare a meno di una riflessione sui differenti significati del vuoto.

  20. The HILW-LL (high- and intermediate-level waste, long-lived) disposal project: working toward building the Cigeo Industrial Centre for Geological Disposal; Le projet HA-MAVL: vers la realisation du centre industriel de stockage geologique Cigeo

    Energy Technology Data Exchange (ETDEWEB)

    Labalette, Th. [Agence Nationale pour la Gestion des Dechets Radioactifs - ANDRA, Dir. des Projets, 92 - Chatenay Malabry (France)

    2011-02-15

    The French Act of 28 June 2006 identifies reversible disposal in deep geological facilities as the benchmark solution for long-term management of high-level waste (HLW) and for intermediate-level long-lived waste (ILW-LL). The Act tasks ANDRA (national agency for the management of radioactive wastes) with the pursuit of studies and research on the choice of a site and the design of the repository, with a view to examining the licence application in 2015 and, provided that the licence is granted, to make the facility operational by 2025. At the end of 2009, ANDRA submitted to the Government its proposals regarding the site and the design of the Industrial Centre for Geological Disposal, known as CIGEO. With the definition of a possible area for the construction of underground disposal facilities, one of the key stages in the project has been achieved. The choice of a surface site will be validated following the public consultation scheduled for the end of 2012. The project is now on the point of entering the definition stage (preliminary design). CIGEO will be a nuclear facility unlike any other. It will be built and operated for a period of over 100 years. For it to be successful, the project must meet certain requirements related to its integration in the local area, industrial planning, safety and reversibility, while also controlling costs. Reversibility is a very important concept that will be defined by law. It is ANDRA's responsibility to ensure that a reasonable balance is found between these different concerns. (author)

  1. Availability and Quality of Family Planning Services in the Democratic Republic of the Congo: High Potential for Improvement.

    Science.gov (United States)

    Mpunga, Dieudonné; Lumbayi, J P; Dikamba, Nelly; Mwembo, Albert; Ali Mapatano, Mala; Wembodinga, Gilbert

    2017-06-27

    To determine the availability and quality of family planning services within health facilities throughout the Democratic Republic of the Congo (DRC). Data were collected for the cross-sectional study from April 2014 to June 2014 by the Ministry of Public Health. A total of 1,568 health facilities that reported data to the National Health Information System were selected by multistage random sampling in the 11 provinces of the DRC existing at that time. Data were collected through interviews, document review, and direct observation. Two dependent variables were measured: availability of family planning services (consisting of a room for services, staff assigned to family planning, and evidence of client use of family planning) and quality of family planning services (assessed as "high" if the facility had at least 1 trained staff member, family planning service delivery guidelines, at least 3 types of methods, and a sphygmomanometer, or "low" if the facility did not meet any of these 4 criteria). Pearson's chi-square test and odds ratios (ORs) were used to test for significant associations, using the alpha significance level of .05. We successfully surveyed 1,555 facilities (99.2%) of those included in the sample. One in every 3 facilities (33%) offered family planning services as assessed by the index of availability, of which 20% met all 4 criteria for providing high-quality services. Availability was greatest at the highest level of the health system (hospitals) and decreased incrementally with each health system level, with disparities between provinces and urban and rural areas. Facilities in urban areas were more likely than in rural areas to meet the standard for high-quality services (Pplanning services in health facilities in the DRC remain low, with inequitable distribution of services throughout the country. To improve access to and use of family planning, efforts should focus on improving availability and quality at lower health system levels and in

  2. Availability of state-based obesity surveillance data on high school students with disabilities in the United States.

    Science.gov (United States)

    Yamaki, Kiyoshi; Lowry, Brienne Davis; Buscaj, Emilie; Zisko, Leigh; Rimmer, James H

    2015-05-01

    The aim of this study was to assess the availability of public health surveillance data on obesity among American children with disabilities in state-based surveillance programs. We reviewed annual cross-sectional datasets in state-level surveillance programs for high school students, implemented 2001-2011, for the inclusion of weight and height and disability screening questions. When datasets included a disability screen, its content and consistency of use across years were examined. We identified 54 surveillance programs with 261 annual datasets containing obesity data. Twelve surveillance programs in 11 states included a disability screening question that could be used to extract obesity data for high school students with disabilities, leaving the other 39 states with no state-level obesity data for students with disabilities. A total of 43 annual datasets, 16.5 % of the available datasets, could be used to estimate the obesity status of students with disabilities. The frequency of use of disability questions varied across states, and the content of the questions often changed across years and within a state. We concluded that state surveillance programs rarely contained questions that could be used to identify high school students with disabilities. This limits the availability of data that can be used to monitor obesity and related health statuses among this population in the majority of states.

  3. Low temperature spark plasma sintering of TC4/HA composites

    Institute of Scientific and Technical Information of China (English)

    Huiliang Shao; Lei Cao; Daqian Sun; Zhankui Zhao

    2016-01-01

    Ti6Al4V/hydroxyapatite composites (TC4/HA) have been prepared by high energy ball milling and low temperature spark plasma sintering at 600 °C, 550 °C, 500 °C and 450 °C, respectively. The sintering temperature of the composites was sharply decreased as the result of the activation and surficial modification effects induced from high energy ball milling. The decomposition and reaction of hydro-xyapatite was successfully avoided, which offers the composites superior biocompatibility. The hydro-xyapatite in the composites was distributed in gap uniformly, and formed an ideal network structure. The lowest hardness, compressive strength and Young's modulus of the composites satisfy the requirements of human bone.

  4. Broad HA wings in young planetary nebulae

    Directory of Open Access Journals (Sweden)

    A. Arrieta

    2002-01-01

    Full Text Available De una muestra de 30 nebulosas planetarias j ovenes, proto-NPs y otros objetos con l neas de emisi on hemos encontrado 11 NPs j ovenes y dos estrellas simbi oticas que muestran la l nea de emisi on H extremadamente ancha (desde 800 km s

  5. Condom availability in high risk places and condom use: a study at district level in Kenya, Tanzania and Zambia

    Directory of Open Access Journals (Sweden)

    Sandøy Ingvild

    2012-11-01

    Full Text Available Abstract Background A number of studies from countries with severe HIV epidemics have found gaps in condom availability, even in places where there is a substantial potential for HIV transmission. Although reported condom use has increased in many African countries, there are often big differences by socioeconomic background. The aim of this study was to assess equity aspects of condom availability and uptake in three African districts to evaluate whether condom programmes are given sufficient priority. Methods Data on condom availability and use was examined in one district in Kenya, one in Tanzania and one in Zambia. The study was based on a triangulation of data collection methods in the three study districts: surveys in venues where people meet new sexual partners, population-based surveys and focus group discussions. The data was collected within an overall study on priority setting in health systems. Results At the time of the survey, condoms were observed in less than half of the high risk venues in two of the three districts and in 60% in the third district. Rural respondents in the population-based surveys perceived condoms to be less available and tended to be less likely to report condom use than urban respondents. Although focus group participants reported that condoms were largely available in their district, they expressed concerns related to the accessibility of free condoms. Conclusion As late as thirty years into the HIV epidemic there are still important gaps in the availability of condoms in places where people meet new sexual partners in these three African districts. Considering that previous studies have found that improved condom availability and accessibility in high risk places have a potential to increase condom use among people with multiple partners, the present study findings indicate that substantial further efforts should be made to secure that condoms are easily accessible in places where sexual relationships are

  6. A critical HA1 neutralizing domain of H5N1 influenza in an optimal conformation induces strong cross-protection.

    Directory of Open Access Journals (Sweden)

    Lanying Du

    Full Text Available The highly pathogenic avian influenza (HPAI H5N1 viruses, especially the laboratory-generated H5N1 mutants, have demonstrated the potential to cross the species barrier and infect mammals and humans. Consequently, the design of an effective and safe anti-H5N1 vaccine is essential. We previously demonstrated that the full-length hemagglutinin 1 (HA1 could induce significant neutralizing antibody response and protection. Here, we intended to identify the critical neutralizing domain (CND in an optimal conformation that can elicit strong cross-neutralizing antibodies and protection against divergent H5N1 strains. We thus constructed six recombinant proteins covering different regions of HA1 of A/Anhui/1/2005(H5N1, each of which was fused with foldon (Fd and Fc of human IgG. We found that the critical fragment fused with Fd/Fc (HA-13-263-Fdc, H5 numbering that could elicit the strongest neutralizing antibody response is located in the N-terminal region of HA1 (residues 13-263, which covers the receptor-binding domain (RBD, residues 112-263. We then constructed three additional recombinants fused with Fd plus His tag (HA-13-263-Fd-His, Fc only (HA-13-263-Fc, and His tag only (HA-13-263-His, respectively. We found that the HA-13-263-Fdc, which formed an oligomeric conformation, induced the strongest neutralizing antibody response and cross-protection against challenges of two tested H5N1 virus strains covering clade 1: A/VietNam/1194/2004 (VN/1194 or clade 2.3.4: A/Shenzhen/406H/06 (SZ/406H, while HA-13-263-Fc dimer and HA-13-263-Fd-His trimer elicited higher neutralizing antibody response and protection than HA-13-263-His monomer. These results suggest that the oligomeric form of the CND containing the RBD can be further developed as an effective and safe vaccine for cross-protection against divergent strains of H5N1 viruses.

  7. Chi ha paura dei neuroni specchio?

    Directory of Open Access Journals (Sweden)

    Daniela Mario

    2012-07-01

    Full Text Available Mi è capitato spesso negli ultimi anni, impegnati ad analizzare scritti utili alla tesi di dottorato (il ruolo del meccanismo mirror nella comprensione del testo, di incontrare titoli riportanti il suffisso "neuro" su articoli e libri degli ambiti di studio più disparati, nel tentativo, suppongo, di riconfigurare temi ritenuti troppo complessi per essere trattati in termini puramente neuroscientifici. “Dubitare di tutto o credere a tutto sono due strategie altrettanto comode. Con entrambe eliminiamo la necessità di riflettere” (H. Poincarè

  8. THE ASSESMENT OF MAIN CHARACTERISTICS AND PROCESSING AVAILABILITY OF WOOL OBTAINED FROM SHEEP BELONGING TO THE PALAS HIGH PROLIFICACY LINE

    Directory of Open Access Journals (Sweden)

    ANA ENCIU

    2013-12-01

    Full Text Available The purpose of these researches was to analyze the quantitative and qualitative parameters of wool obtained from the Palas sheep population with high prolificacy and to assess the processing availabilities of these fibres type. Average values recorded for the main wool traits ranged between the following limits: wool production 2.86 ÷ 4.72 kilos; fibres fineness 27.28 ÷ 29.32 microns; variability of fineness ( CV 26.88 ÷ 29.94 %; staple length 9.77 ÷ 14.23 cm, variability of length ( CV 18.01 ÷ 22.06 % ; scouring yield 54.05 ÷ 61.44 % and the variability of yield 8.35 ÷ 14.78 %. . This wool has a technological value similar to that obtained from the sheep breed with medium fineness wool (Tigaie, Spanca .

  9. Titanium Dioxide Nanoparticle Penetration into the Skin and Effects on HaCaT Cells

    Directory of Open Access Journals (Sweden)

    Matteo Crosera

    2015-08-01

    Full Text Available Titanium dioxide nanoparticles (TiO2NPs suspensions (concentration 1.0 g/L in synthetic sweat solution were applied on Franz cells for 24 h using intact and needle-abraded human skin. Titanium content into skin and receiving phases was determined. Cytotoxicity (MTT, AlamarBlue® and propidium iodide, PI, uptake assays was evaluated on HaCat keratinocytes after 24 h, 48 h, and seven days of exposure. After 24 h of exposure, no titanium was detectable in receiving solutions for both intact and damaged skin. Titanium was found in the epidermal layer after 24 h of exposure (0.47 ± 0.33 μg/cm2 while in the dermal layer, the concentration was below the limit of detection. Damaged skin, in its whole, has shown a similar concentration (0.53 ± 0.26 μg/cm2. Cytotoxicity studies on HaCaT cells demonstrated that TiO2NPs induced cytotoxic effects only at very high concentrations, reducing cell viability after seven days of exposure with EC50s of 8.8 × 10−4 M (MTT assay, 3.8 × 10−5 M (AlamarBlue® assay, and 7.6 × 10−4 M (PI uptake, index of a necrotic cell death. Our study demonstrated that TiO2NPs cannot permeate intact and damaged skin and can be found only in the stratum corneum and epidermis. Moreover, the low cytotoxic effect observed on human HaCaT keratinocytes suggests that these nano-compounds have a potential toxic effect at the skin level only after long-term exposure.

  10. [Not Available].

    Science.gov (United States)

    Navarro-González, Inmaculada; Ros, Gaspar; Martínez-García, Brígida; Rodríguez-Tadeo, Alejandra; Periago, M Jesús

    2016-07-19

    Introducción y objetivos: la evolución que ha experimentado en los últimos años la sociedad española ha originado una serie de cambios sociológicos y/o culturales que afectan claramente a los hábitos y preferencias alimentarias. El objetivo del presente trabajo ha sido evaluar la adherencia a la dieta mediterránea (DM) de la población universitaria en la ciudad de Murcia y la calidad de su desayuno, estableciendo la relación existente entre estas dos variables entre sí, y otras variables sociológicas analizadas.Métodos: se evaluó la adherencia a la DM en una muestra de 312 estudiantes mediante el test KIDMED, cuestionario de 16 preguntas. La calidad del desayuno fue determinada en función de la ingesta de los distintos grupos de alimentos (lácteos, cereales y frutas), de forma que se establecieron las siguientes categorías de calidad: pésima, mala, regular y buena. De cada encuestado se registraron los datos personales, lugar de origen, zona geográfica y tipo de residencia, sexo, edad, titulación universitaria, curso y las variables antropométricas (peso, altura, perímetro de cintura y cadera).Resultados: la muestra estuvo formada por 238 mujeres y 74 hombres todos ellos estudiantes universitarios de distintos títulos de grado. El 7,85% de los universitarios mostró una baja adherencia a la DM, el 48,4% media y el 43,8% alta. Los estudiantes de grado en Educación Primaria mostraron una mayor adherencia media a la DM; los estudiantes de grado en Enfermería tomaron más de una ración de verdura al día; y los de Ciencia y Tecnología de los Alimentos consumían una pieza de fruta al día. Teniendo en cuenta el sexo de los participantes, el 37,8% de las mujeres tomaban más de una verdura diaria y el 91,5% desayunaba bollería industrial; mientras que el 54,1% de los hombres tomaban frutos secos (al menos 2 o 3 veces por semana) y el 74,5% desayunaba todos los días. El 1,15% de los estudiantes mostró una calidad del desayuno pésima, el 17

  11. HA/UHMWPE Nanocomposite Produced by Twin-screw Extrusion

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The HA/UHMWPE nanocomposite is compounded by twin-screw extrusion of the HA and UHMWPE powder mixture in paraffin oil and then compression molded to a sheet form. TGA measurement shows the HA weight loss after processing is about 1%-2% . FTIR spectra indicate the paraffin oil residue is trivial and UHMWPE is not oxidized. SEM reveals the HA nano particles are homogeneously dispersed by twin- screw extrusion and the inter-particle spaces are penetrated with UHMWPE fibrils by swelling treatment. HRTEM image indicates the HA particles and UHMWPE are intimately contacted by mechanical interlocking. Compared with the unfilled UHMWPE, stiffness of the composite with the HA volume fraction 0.23 was significantly enhanced to 9 times without detriment of the yield strength and the ductility.

  12. Next Generation Fast RF Interlock Module and ATCA Adapter for ILC High Availability RF Test Station Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, R

    2009-10-17

    High availability interlocks and controls are required for the ILC (International Linear Collider) L-Band high power RF stations. A new F3 (Fast Fault Finder) VME module has been developed to process both fast and slow interlocks using FPGA logic to detect the interlock trip excursions. This combination eliminates the need for separate PLC (Programmable Logic Controller) control of slow interlocks. Modules are chained together to accommodate as many inputs as needed. In the next phase of development the F3's will be ported to the new industry standard ATCA (Advanced Telecom Computing Architecture) crate (shelf) via a specially designed VME adapter module with IPMI (Intelligent Platform Management Interface). The goal is to demonstrate auto-failover and hot-swap for future partially redundant systems.

  13. LHCb: Performance evaluation and capacity planning for a scalable and highly available virtulization infrastructure for the LHCb experiment

    CERN Multimedia

    Sborzacchi, F; Neufeld, N

    2013-01-01

    The virtual computing is often run to satisfy different needs: reduce costs, reduce resources, simplify maintenance and the last but not the least add flexibility. The use of Virtualization in a complex system such as a farm of PCs that control the hardware of an experiment (PLC, power supplies ,gas, magnets..) put us in a condition where not only an High Performance requirements need to be carefully considered but also a deep analysis of strategies to achieve a certain level of High Availability. We conducted a performance evaluation on different and comparable storage/network/virtulization platforms. The performance is measured using a series of independent benchmarks , testing the speed an the stability of multiple VMs runnng heavy-load operations on the I/O of virtualized storage and the virtualized network. The result from the benchmark tests allowed us to study and evaluate how the different workloads of Vm interact with the Hardware/Software resource layers.

  14. ScreenMill: A freely available software suite for growth measurement, analysis and visualization of high-throughput screen data

    Directory of Open Access Journals (Sweden)

    Rothstein Rodney

    2010-06-01

    Full Text Available Abstract Background Many high-throughput genomic experiments, such as Synthetic Genetic Array and yeast two-hybrid, use colony growth on solid media as a screen metric. These experiments routinely generate over 100,000 data points, making data analysis a time consuming and painstaking process. Here we describe ScreenMill, a new software suite that automates image analysis and simplifies data review and analysis for high-throughput biological experiments. Results The ScreenMill, software suite includes three software tools or "engines": an open source Colony Measurement Engine (CM Engine to quantitate colony growth data from plate images, a web-based Data Review Engine (DR Engine to validate and analyze quantitative screen data, and a web-based Statistics Visualization Engine (SV Engine to visualize screen data with statistical information overlaid. The methods and software described here can be applied to any screen in which growth is measured by colony size. In addition, the DR Engine and SV Engine can be used to visualize and analyze other types of quantitative high-throughput data. Conclusions ScreenMill automates quantification, analysis and visualization of high-throughput screen data. The algorithms implemented in ScreenMill are transparent allowing users to be confident about the results ScreenMill produces. Taken together, the tools of ScreenMill offer biologists a simple and flexible way of analyzing their data, without requiring programming skills.

  15. Bør leger ha reservasjonsrett ved assistert befruktning?

    Directory of Open Access Journals (Sweden)

    Morten Magelssen

    2011-10-01

    Full Text Available Omfanget av helsepersonells reservasjonsrett har nylig vært gjenstand for debatt i Norge. Vi spør om leger bør ha reservasjonsrett ved utførelse og henvisning til assistert befruktning, og drøfter argumenter for og imot ved hjelp av et rammeverk med sju kriterier for vurdering av reservasjon. Reservasjonsrettens grunnleggende dilemma er hvordan to viktige hensyn, henholdsvis pasientens rett til behandling og hensynet til helsepersonellets moralske integritet, best kan ivaretas. Det argumenteres for at leger bør ha rett til å reservere seg mot å utføre, assistere ved og henvise til assistert befruktning generelt hvis begrunnelsen er hensynet til befruktede eggs moralske verdi. Videre finner vi at leger også kan ha en moralsk rett til reservasjon mot å utføre, assistere ved og henvise til assistert befruktning for likekjønnede, men da på nærmere spesifiserte vilkår.Nøkkelord: reservasjonsrett, assistert befruktning, samvittighet, moralsk integritetEnglish summary: Should physicians have the right to conscientiously object to assisted reproduction?The extent of the healthcare worker's right to conscientious objection has recently been debated in Norway. This article asks whether physicians should have a right to conscientious objection to the performance of, and referral for, assisted reproduction, and discusses arguments for and against the same, utilizing a framework of seven criteria for the evaluation of conscientious objection. The fundamental dilemma of conscientious objection is how two important considerations can be reconciled: the patient's right to treatment, and the protection of the healthcare worker's moral integrity. It is argued that physicians should have the right to object to performing, assisting with, and referring for assisted reproduction generally when the objection is grounded in the moral value of the embryo. Furthermore, physicians may also have a moral right to object to performing, assisting with, and

  16. Se il filosofo ha un compito

    Directory of Open Access Journals (Sweden)

    Carmine Di Martino

    2011-01-01

    Full Text Available Il testo muove da una ricognizione dello “stile” complessivo dell’interrogazione filosofica contemporanea: stile decostruttivo, che si risolve in un’illustrazione del limite istitutivo e costitutivo di ogni dire filosofico (e non solo filosofico. In un secondo tempo del suo intervento, l’autore si chiede se una tale comprensione, puramente “negativa”, di quel limite e della validità della parola che ne discende, non sia essa stessa ideologica, e se non sia possibile frequentare una definizione positiva di tale limite, e una possibilità non soltanto autodecostruttiva della parola filosofica “dopo” Nietzsche, Heidegger, Derrida.

  17. Improved virus removal by high-basicity polyaluminum coagulants compared to commercially available aluminum-based coagulants.

    Science.gov (United States)

    Shirasaki, N; Matsushita, T; Matsui, Y; Oshiba, A; Marubayashi, T; Sato, S

    2014-01-01

    We investigated the effects of basicity, sulfate content, and aluminum hydrolyte species on the ability of polyaluminum chloride (PACl) coagulants to remove F-specific RNA bacteriophages from river water at a pH range of 6-8. An increase in PACl basicity from 1.5 to 2.1 and the absence of sulfate led to a reduction of the amount of monomeric aluminum species (i.e., an increase of the total amount of polymeric aluminum and colloidal aluminum species) in the PACl, to an increase in the colloid charge density of the PACl, or to both and, as a result, to high virus removal efficiency. The efficiency of virus removal at around pH 8 observed with PACl-2.1c, a nonsulfated high-basicity PACl (basicity 2.1-2.2) with a high colloidal aluminum content, was larger than that observed with PACl-2.1b, a nonsulfated high-basicity PACl (basicity 2.1-2.2) with a high polymeric aluminum content. In contrast, although extremely high basicity PACls (e.g., PACl-2.7ns, basicity 2.7) effectively removed turbidity and UV260-absorbing natural organic matter and resulted in a very low residual aluminum concentration, the virus removal ratio with PACl-2.7ns was smaller than the ratio with PACl-2.1c at around pH 8, possibly as a result of a reduction of the colloid charge density of the PACl as the basicity was increased from 2.1 to 2.7. Liquid (27)Al NMR analysis revealed that PACl-2.1c contained Al30 species, which was not the case for PACl-2.1b or PACl-2.7ns. This result suggests that Al30 species probably played a major role in virus removal during the coagulation process. In summary, PACl-2.1c, which has high colloidal aluminum content, contains Al30 species, and has a high colloid charge density, removed viruses more efficiently (>4 log10 for infectious viruses) than the other aluminum-based coagulants-including commercially available PACls (basicity 1.5-1.8), alum, and PACl-2.7ns-over the entire tested pH (6-8) and coagulant dosage (0.54-5.4 mg-Al/L) ranges.

  18. [Not Available].

    Science.gov (United States)

    Pérez-Flores, Juan Emmanuel; Chávez-Tostado, Mariana; Larios-Del-Toro, Youné Elizabeth; García-Rentería, Jesús; Rendrón-Félix, Jorge; Salazar-Parra, Marcela; Irusteta-Jiménez, Leire; Michel-Espinoza, Luis Rodrigo; Márquez-Valdez, Aída Rebeca; Cuesta-Márquez, Lisbeth; Álvarez-Villaseñor, Andrea Socorro; Fuentes-Orozco, Clotilde; González Ojeda, Alejandro

    2016-07-19

    Introducción: la desnutrición intrahospitalaria se ha descrito hace más de 70 años como un problema frecuente. En México se reportan cifras de entre el 20% al 50%; sin embargo no se ha estudiado su prevalencia ni su asociación con la morbilidad y mortalidad hospitalaria.Objetivos: evaluar el estado nutricional y su relación con la morbimortalidad hospitalaria en pacientes mexicanos.Métodos: cohorte prospectiva de pacientes que ingresaron en un hospital de referencia para una estancia hospitalaria mayor de 5 días. Se capturó peso, talla, índice de masa corporal (IMC), estado nutricional de acuerdo con la valoración global subjetiva (VGS) a su ingreso y egreso hospitalario, así como diagnóstico médico, complicaciones y mortalidad. Los datos fueron analizados mediante la prueba T de Student, prueba Chi-cuadrado y prueba Exacta de Fisher.Resultados: se incluyeron 610 pacientes en total, con un promedio de edad de 50,8 ± 17,32 años, 267 mujeres (43,8%) y 343 hombres (56,2%). Del total, 154 fueron catalogados con sospecha de desnutrición o desnutrición (pacientes expuestos, 25,2%) y 456 bien nutridos (pacientes no expuestos, 74,8%), con una relación de 1 a 3. La morbilidad total de la cohorte tuvo un RR = 2,70, IC 95 % (2,06-3,55) y la mortalidad con un RR = 2,64, IC 95% (1,74-4,0), siendo ambas estadísticamente significativas (p = 0,001).Conclusiones: el diagnóstico de desnutrición al ingreso hospitalario constituye un factor de riesgo para el desarrollo de complicaciones y mortalidad. Este padecimiento al ingreso en comparación con el paciente que no presenta desnutrición incrementó el riesgo de mortalidad hasta en 2.64 veces.

  19. High-power ultrafast Yb:fiber laser frequency combs using commercially available components and basic fiber tools

    Science.gov (United States)

    Li, Xinlong; Reber, Melanie A. R.; Corder, Christopher; Chen, Yuning; Zhao, Peng; Allison, Thomas K.

    2016-09-01

    We present a detailed description of the design, construction, and performance of high-power ultrafast Yb:fiber laser frequency combs in operation in our laboratory. We discuss two such laser systems: an 87 MHz, 9 W, 85 fs laser operating at 1060 nm and an 87 MHz, 80 W, 155 fs laser operating at 1035 nm. Both are constructed using low-cost, commercially available components, and can be assembled using only basic tools for cleaving and splicing single-mode fibers. We describe practical methods for achieving and characterizing low-noise single-pulse operation and long-term stability from Yb:fiber oscillators based on nonlinear polarization evolution. Stabilization of the combs using a variety of transducers, including a new method for tuning the carrier-envelope offset frequency, is discussed. High average power is achieved through chirped-pulse amplification in simple fiber amplifiers based on double-clad photonic crystal fibers. We describe the use of these combs in several applications, including ultrasensitive femtosecond time-resolved spectroscopy and cavity-enhanced high-order harmonic generation.

  20. Isolation and identiifcation of Serratia marcescens Ha1 and herbicidal activity of Ha1‘pesta’ granular formulation

    Institute of Scientific and Technical Information of China (English)

    YANG Juan; WANG Wei; YANG Peng; TAO Bu; YANG Zheng; ZHANG Li-hui; DONG Jin-gao

    2015-01-01

    A total of 479 bacterial strains were isolated from brine (Bohai, Qinhuangdao City, Hebei Province, China). Bioassay results indicated that 4 strains named Ha1, Ha17, Ha38, and Ha384 had herbicidal activity. And strain Ha1 had the highest effective herbicidal activity. As a result, this study aims to identify strain Ha1, characterize its physiological and biological activities, evaluate the herbicidal activity of its metabolites, and develop a‘pesta’ formulation and assess its effectiveness on Digitaria sanguinalis. Ha1 was identiifed as Serratia marcescens based on 16S rDNA sequencing. This strain has a lfagel um, a diameter of 0.5 to 0.8μm, and a length of 0.9 to 2.0μm. The indole test shows positive results, and the catalase enzyme exhibits strong positive reactions. Results further showed that the inhibitory concentration (IC50) of the crude extracts to D. sanguinalis radicula and coleoptile were 3.332 and 2.828 mg mL–1, respectively. Both the suppression of D. sanguinalis and the cel viability of the Ha1 formulation in‘pesta’ were higher when stored at 4°C than at (25±2)°C. These results indi-cated that S. marcescens Ha1 can potential y be used as a biocontrol agent against D. sanguinalis.

  1. Võidupüha 8. mail? / Leonti Kährik

    Index Scriptorium Estoniae

    Kährik, Leonti

    2005-01-01

    Eesti peaks väärikalt tähistama võidupüha kui euroopalike demokraatlike väärtuste võitu natsismi ja fašismi üle. Lähtudes 1940. a. juunini Eestis kehtinud vööndiajast, võiks võidupüha tähistada 8. mail

  2. Võidupüha 8. mail? / Leonti Kährik

    Index Scriptorium Estoniae

    Kährik, Leonti

    2005-01-01

    Eesti peaks väärikalt tähistama võidupüha kui euroopalike demokraatlike väärtuste võitu natsismi ja fašismi üle. Lähtudes 1940. a. juunini Eestis kehtinud vööndiajast, võiks võidupüha tähistada 8. mail

  3. ha Platoni ordeni sai kaks saarlast / Isa Andreas

    Index Scriptorium Estoniae

    Andreas, Isa

    2009-01-01

    ha Platoni päeval päeval jagas metropoliit Stefanus püha Platoni ordeneid aktiivsetele kirikutegelastele ning pühitseti Tartu piiskopiks arhimandriit Eelija(Ojaperv) ja Pärnu ja Saare piiskopiks arhimandriit Aleksander (Hopjorski)

  4. ha Platoni ordeni sai kaks saarlast / Isa Andreas

    Index Scriptorium Estoniae

    Andreas, Isa

    2009-01-01

    ha Platoni päeval päeval jagas metropoliit Stefanus püha Platoni ordeneid aktiivsetele kirikutegelastele ning pühitseti Tartu piiskopiks arhimandriit Eelija(Ojaperv) ja Pärnu ja Saare piiskopiks arhimandriit Aleksander (Hopjorski)

  5. [Not Available].

    Science.gov (United States)

    San Mauro Martín, Ismael; Garicano Vilar, Elena; León Fernández, Joana; Ciudad Cabañas, María José; Collado Yurrita, Luis

    2016-07-19

    Introducción: recientemente ha surgido el interés por el posible papel de la microbiota intestinal como agente potenciador del rápido aumento que se está observando en el aumento de la prevalencia de obesidad en todo el mundo.Objetivos: relacionar las modificaciones que puede ocasionar el consumo de antibióticos en la microflora intestinal con la obesidad.Métodos: se extrajeron, de las Encuestas Nacionales de Salud de España, entre los años 2001 y 2011, las cifras de ingesta de antibióticos y la prevalencia (%) de obesidad en el mismo rango de edad, género y año. La media total de encuestados fue de 29.904 participantes.Resultados: se encontraron correlaciones significativas (p=0,09) en la asociación de la prevalencia de obesidad y la ingesta de antibióticos. Además, se objetivó que en el sexo masculino esta influencia es el doble que en el femenino.Conclusiones: los descubrimientos recientes en el campo de la microbiota intestinal y su relación con el huésped abren nuevos caminos en la comprensión de ciertas enfermedades inflamatorias y metabólicas, como la obesidad.

  6. [Not Available].

    Science.gov (United States)

    Fernández Castillo, Rafael; Cañadas de la Fuente, Gustavo R; Cañadas de la Fuente, Guillermo A; De la Fuente Solana, Emilia Inmaculada; Esteban de la Rosa, Rafael José; Bravo Soto, Juan

    2016-07-19

    Introducción: la obesidad y el sobrepeso presentan efectos adversos sobre la salud, lo que contribuye a la aparición de enfermedades metabólicas y cardiovasculares que ponen en peligro la integridad del injerto.Objetivo: investigar la influencia del IMC pretrasplante renal sobre el funcionamiento del injerto renal al año de trasplante mediante el estudio de cuatro métodos distintos de medir la filtración glomerular.Material y métodos: en este trabajo se ha seguido a 1.336 pacientes de ambos sexos trasplantados renales; se les realizaron mediciones pretrasplante y postrasplante de parámetros bioquímicos, mediciones antropométricas y función renal mediante medidas de filtrado glomerular.Resultados: a mayor índice de masa corporal pretrasplante se produce una disminución del filtrado glomerular medido por cuatro métodos distintos, así como mayor porcentaje de rechazos.Conclusiones: un IMC elevado pretrasplante contribuye a la disfunción del injerto, a una disminución del filtrado glomerular y a complicaciones del injerto en el primer año postrasplante.

  7. [Not Available].

    Science.gov (United States)

    Fort Casamartina, Eduard; Arribas Hortiguela, Lorena; Bleda Pérez, Carmen; Muñoz Sánchez, Carme; Peiro Martínez, Imma; Perayre Badía, María; Clopés Estela, Ana

    2016-06-03

    Durante la última década, el conocimiento de nuevos mecanismos implicados en el desarrollo del cáncer ha permitido el diseño de nuevos fármacos para su tratamiento y la mayor parte de ellos son fármacos que se administran por vía oral. Uno de los principales problemas de los pacientes oncohematológicos es la desnutrición, que suele tener causas multifactoriales (de la propia enfermedad, de los pacientes y de los diferentes tratamientos administrados). Para minimizar el impacto de la desnutrición es necesaria una intervención nutricional, ya sea adaptando la dieta o mediante la instauración de soporte nutricional artificial, en función de la gravedad de cada caso. En cualquier paciente que esté recibiendo un tratamiento oncológico hay que evaluar las posibles interacciones que pueden existir con el soporte nutricional instaurado, ya sea dieta oral, suplementación oral o nutrición enteral. Estas interacciones pueden disminuir la eficacia, aumentar la toxicidad de los tratamientos o producir déficits nutricionales. Se detallan las principales interacciones que se pueden producir, las interacciones entre los tratamientos oncológicos y el soporte nutricional.

  8. [Not Available].

    Science.gov (United States)

    Torres Díaz, Cristina V; Martín Peña, Gonzalo; Ezquiaga, Elena; Navas García, Marta; García de Sola, Rafael

    2016-07-19

    Gracias a los avances técnicos en técnicas neuroquirúrgicas, y debido a que el diagnóstico y la clasificación de las enfermedades psiquiátricas han evolucionado significativamente a lo largo de las últimas décadas, se están desarrollando tratamientos a nivel experimental para aquellos pacientes resistentes al manejo conservador.La anorexia nerviosa es una enfermedad de prevalencia creciente, con la tasa de mortalidad más elevada dentro de los trastornos psiquiátricos, y con aproximadamente un 20% de pacientes que presentan una evolución tórpida. Para estos pacientes que no responden a manejo conservador, la estimulación cerebral profunda ha surgido como una alternativa terapéutica, si bien la literatura especializada al respecto es escasa.A continuación presentamos una revisión de la fisiopatología de la anorexia nerviosa, así como de los distintos tratamientos neuroquirúrgicos realizados a lo largo de la historia. Se detalla la perspectiva de tratamiento quirúrgico actual, así como los aspectos éticos que se han de considerar en relación con el surgimiento de estas nuevas terapias.

  9. High phosphate availability as a possible cause for massive cyanobacterial production of oxygen in the Paleoproterozoic atmosphere

    Science.gov (United States)

    Papineau, Dominic; Purohit, Ritesh; Fogel, Marilyn L.; Shields-Zhou, Graham A.

    2013-01-01

    have arisen from the biological response to high phosphate availability. We conclude that increased phosphate availability during and after the Paleoproterozoic Lomagundi-Jatuli event likely caused cyanobacterial blooms and was a key factor in the oxygenation of Earth's atmosphere. Increasing oxygenation of the shallow ocean seafloor favored the removal of excess phosphate as authigenic apatite, thus dampening effects of weathering increases on organic burial and marine δ13Ccarb after about 2.0 Ga.

  10. Statistical analysis of the limitation of half integer resonances on the available momentum acceptance of the High Energy Photon Source

    Science.gov (United States)

    Jiao, Yi; Duan, Zhe

    2017-01-01

    In a diffraction-limited storage ring, half integer resonances can have strong effects on the beam dynamics, associated with the large detuning terms from the strong focusing and strong sextupoles as required for an ultralow emittance. In this study, the limitation of half integer resonances on the available momentum acceptance (MA) was statistically analyzed based on one design of the High Energy Photon Source (HEPS). It was found that the probability of MA reduction due to crossing of half integer resonances is closely correlated with the level of beta beats at the nominal tunes, but independent of the error sources. The analysis indicated that for the presented HEPS lattice design, the rms amplitude of beta beats should be kept below 1.5% horizontally and 2.5% vertically to reach a small MA reduction probability of about 1%.

  11. Identification of Microorganisms in Duck Meat Products Available in Korea and the Effect of High Hydrostatic Pressure.

    Science.gov (United States)

    Kim, Hyun-Joo; Yong, Hae In; Lee, Hyun Jung; Jung, Samooel; Kwon, Joong-Ho; Heo, Kang Nyung; Jo, Cheorun

    2016-01-01

    The objective of this study was to investigate the microbial count of duck meat and duck meat products commercially available in Korea. High hydrostatic pressure (HHP) treatment was applied at 0.1, 300, 400, and 500 MPa for 5 min to enhance the microbiological safety of duck meats. The levels of total aerobic bacteria were in the ranges of 3.53-6.19 and 3.62-6.85 Log CFU/g in raw and smoked duck products, respectively. By DNA sequence analysis, we identified microorganisms responsible for spoilage, with the most common species in the raw and smoked duck products being Aeromonas spp. or Pseudomonas spp. and Leuconostoc mesenteroides, respectively. HHP treatment significantly reduced the levels of total aerobic bacteria in raw and smoked duck products. This study demonstrates that HHP treatment may be used to effectively improve the safety of raw and smoked duck meat products.

  12. New therapeutic agent for radiation synovectomy - preparation of {sup 166}Ho-EDTMP-HA particle

    Energy Technology Data Exchange (ETDEWEB)

    Bai, H.; Jin, X.; Du, J.; Wang, F.; Chen, D.; Fan, H.; Cheng, Z.; Zhang, J. [China Institute of Atomic Energy, Beijing (Switzerland). Isotope Department

    1997-10-01

    In order to prepare new therapeutical agent for radiation synovectomy, Hydroxyapatite (HA) was labelled with {sup 166}Ho by EDTMP that had high affinity to HA particles. Radiolabelling of HA particles was divided into two steps, {sup 166}Ho-EDTMP was prepared first; then mixed with HA particles completely and vibrated for 15 minutes on the micromixer at room temperature, washed 3 times with deionized water. Radiolabelling particle was separated from free {sup 166}Ho via centrifugation to determine its radiolabelling efficiency. {sup 166}Ho-EDTMP-HA and {sup 166}Ho-EDTMP were injected into knee joint of normal rabbits respectively, every group was killed at different time postinjection, took out major organ and collected urine and blood, then weighted and determined their radio counts. HA particles, as a natural component of bone was known to have good compatibility with soft tissue and biodegrade into calcium and phosphate in vivo. It was readily prepared from common chemical and formed into particles of desired size range in a controlled process, it had high stability in vitro and vivo. Radiolabelling of HA particle with {sup 166}Ho by EDTMP was simple to perform and provides an excellent labelling yield that was more than 95% under the optimal labelling condition. The optimal labelling condition at room temperature was pH 6.0-8.0 and vibration time 15 minutes. The absorbed capacity of HA particle was 5 mg Ho/g HA particle and size of radiolabelling particle was at range of 2-5,{mu}m that is suitable for therapy of radiation synovectomy. {sup 166}Ho-EDTMP-HA particle demonstrated high in vitro stability in either normal saline or 1% BSA solution, but instability under extremely acidic condition (pH 1-2). The control studies performed with {sup 166}Ho-EDTMP not bound to HA particle provided information on the distribution of radioactivity that would occur upon leakage of the radiochemical compound from joint. Its short half-life, its extremely low leakage from the

  13. Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.

    Science.gov (United States)

    Mulloy, Barbara; Wu, Nian; Gyapon-Quast, Frederick; Lin, Lei; Zhang, Fuming; Pickering, Matthew C; Linhardt, Robert J; Feizi, Ten; Chai, Wengang

    2016-07-05

    Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes. HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN). Commercially available HS preparations have been invaluable in many types of research activities. In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS. Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively. (1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine. The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place. HS is widely used by the scientific community to investigate HS structures and activities. Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis. Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.

  14. Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate

    Science.gov (United States)

    2016-01-01

    Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes. HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN). Commercially available HS preparations have been invaluable in many types of research activities. In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS. Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively. 1H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine. The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place. HS is widely used by the scientific community to investigate HS structures and activities. Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis. Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies. PMID:27295282

  15. Preparation of bioactive porous HA/PCL composite scaffolds

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, J.; Guo, L.Y.; Yang, X.B. [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China); Weng, J. [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China)], E-mail: jweng@swjtu.cn

    2008-12-30

    Porous hydroxyapatite (HA) bioceramic scaffold has been widely attracted the attention to act as a three-dimensional (3D) template for cell adhesion, proliferation, differentiation and thus promoting bone and cartilage regeneration because of its osteoinduction. However, the porous bioceramic scaffold is fragile so that it is not suitable to be applied in clinic for bone repair or replacement. Therefore, it is significant to improve the mechanical property of porous HA bioceramics while the interconnected structure is maintained for tissue ingrowth in vivo. In the present research, a porous composite scaffold composed of HA scaffold and polycaprolactone (PCL) lining was fabricated by the method of polymer impregnating to produce HA scaffold coated with PCL lining. Subsequently, the composite scaffolds were deposited with biomimetic coating for improving the bioactivity. The HA/PCL composite scaffolds with improved mechanical property and bioactivity is expected to be a promising bone substitute in tissue engineering applications.

  16. Preparation of bioactive porous HA/PCL composite scaffolds

    Science.gov (United States)

    Zhao, J.; Guo, L. Y.; Yang, X. B.; Weng, J.

    2008-12-01

    Porous hydroxyapatite (HA) bioceramic scaffold has been widely attracted the attention to act as a three-dimensional (3D) template for cell adhesion, proliferation, differentiation and thus promoting bone and cartilage regeneration because of its osteoinduction. However, the porous bioceramic scaffold is fragile so that it is not suitable to be applied in clinic for bone repair or replacement. Therefore, it is significant to improve the mechanical property of porous HA bioceramics while the interconnected structure is maintained for tissue ingrowth in vivo. In the present research, a porous composite scaffold composed of HA scaffold and polycaprolactone (PCL) lining was fabricated by the method of polymer impregnating to produce HA scaffold coated with PCL lining. Subsequently, the composite scaffolds were deposited with biomimetic coating for improving the bioactivity. The HA/PCL composite scaffolds with improved mechanical property and bioactivity is expected to be a promising bone substitute in tissue engineering applications.

  17. Incorporating wind availability into land use regression modelling of air quality in mountainous high-density urban environment.

    Science.gov (United States)

    Shi, Yuan; Lau, Kevin Ka-Lun; Ng, Edward

    2017-08-01

    Urban air quality serves as an important function of the quality of urban life. Land use regression (LUR) modelling of air quality is essential for conducting health impacts assessment but more challenging in mountainous high-density urban scenario due to the complexities of the urban environment. In this study, a total of 21 LUR models are developed for seven kinds of air pollutants (gaseous air pollutants CO, NO2, NOx, O3, SO2 and particulate air pollutants PM2.5, PM10) with reference to three different time periods (summertime, wintertime and annual average of 5-year long-term hourly monitoring data from local air quality monitoring network) in Hong Kong. Under the mountainous high-density urban scenario, we improved the traditional LUR modelling method by incorporating wind availability information into LUR modelling based on surface geomorphometrical analysis. As a result, 269 independent variables were examined to develop the LUR models by using the "ADDRESS" independent variable selection method and stepwise multiple linear regression (MLR). Cross validation has been performed for each resultant model. The results show that wind-related variables are included in most of the resultant models as statistically significant independent variables. Compared with the traditional method, a maximum increase of 20% was achieved in the prediction performance of annual averaged NO2 concentration level by incorporating wind-related variables into LUR model development. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. Favorable cardio-metabolic outcomes following high carbohydrate intake in accordance with the Daniel Fast: A review of available findings

    Directory of Open Access Journals (Sweden)

    Richard Bloomer

    2017-03-01

    Full Text Available The Daniel Fast is a biblically inspired dietary program rich in carbohydrate, most closely resembling a vegan diet but with additional restrictions, including the elimination of processed foods, white flour products, preservatives, additives, sweeteners, caffeine, and alcohol. While no specific requirements are placed on the ingestion of specific percentages of macronutrients, the mean daily carbohydrate intake is by default approximately 60%, while protein and fat intake are 15% and 25%, respectively. Despite a relatively high carbohydrate intake, multiple favorable cardio-metabolic effects are noted when following the plan, in as few as three weeks. This includes improvements in HOMA-IR, which may be at least in part due to the lower glycemic load and high dietary fiber content of the foods consumed. Other notable changes include reductions in systemic inflammation, total and LDL-cholesterol, oxidative stress, blood pressure, and body weight/body fat. Short and moderate-term compliance to the program is excellent-better than most dietary programs, perhaps due to the ad libitum nature of this plan. This paper presents an overview of the Daniel Fast, a carbohydrate-rich dietary program, including relevant findings from both human and animal investigations using this dietary model.

  19. High-power ultrafast Yb:fiber laser frequency combs using commercially available components and basic fiber tools

    CERN Document Server

    Li, X L; Corder, C; Chen, Y; Zhao, P; Allison, T K

    2016-01-01

    We present a detailed description of the design, construction, and performance of high-power ultrafast Yb:fiber laser frequency combs in operation in our laboratory. We discuss two such laser systems: an 87 MHz, 9 W, 85 fs laser operating at 1060 nm and an 87 MHz, 80 W, 155 fs laser operating at 1035 nm. Both are constructed using low-cost, commercially available components, and can be assembled using only basic tools for cleaving and splicing single-mode fibers. We describe practical methods for achieving and characterizing low-noise single-pulse operation and long-term stability from Yb:fiber oscillators based on nonlinear polarization evolution. Stabilization of the combs using a variety of transducers, including a new method for tuning the carrier-envelope offset frequency, is discussed. High average power is achieved through chirped-pulse amplification in simple fiber amplifiers based on double-clad photonic crystal fibers. We describe the use of these combs in several applications, including ultrasensit...

  20. A Study of Hybrid Composite Hydroxyapatite (HA-Geopolymers as a Material for Biomedical Application

    Directory of Open Access Journals (Sweden)

    Saleha

    2017-01-01

    Full Text Available The main purpose of this research is to study the physical properties and microstructure characters of hybrid composites HA-geopolymers as a material for biomedical application. Hybrid composite HA–geopolymers were produced through alkaline activation method of metakaolin as a matrix and HA as the filler. HA was synthesized from eggshell particles by using a precipitation method. The addition of HA in metakaolin paste was varied from 0.5%, 1.0%, 1.5%, and 2.0% relative the weight of metakaolin. FTIR was used to examine the absorption bands the composites. X-ray diffraction (XRD was used to study the crystal structure of the starting and the resulting materials. Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS was used to investigate the surface morphology of the composites. The thermal properties of the samples was examined by means of Differential Scanning Calorimetry (DSC. Capacitance measurement was conducted to investigate the bioactive properties of HA. The study results suggest that hybrid composite HA-geopolymers has a potential to be applied as a biomedical such as biosensor material.

  1. [Not Available].

    Science.gov (United States)

    Campos Jara, Christian; Delgado Floody, Pedro; Caamaño Navarrete, Felipe; Guzmán Guzmán, Iris; Cesp Barría, Mauricio; Jerez Mayorga, Daniel; Alarcón Hormazábal, Manuel; Osorio Poblete, Aldo

    2016-07-19

    Antecedentes: la prevalencia de obesidad infantil ha aumentado considerablemente en Chile y el mundo, presentando asociación negativa con el rendimiento físico. Objetivo: el propósito del estudio fue determinar el rendimiento físico en escolares y asociarlos a los niveles de sobrepeso, obesidad y riesgo cardiometabólico.Material y métodos: 342 escolares, entre 11 y 16 años de edad, participaron del estudio, un 44,2% correspondió a mujeres y un 55,8% a hombres. Se evaluaron las siguientes variables: peso, índice de masa corporal, porcentaje de masa grasa, contorno cintura, razón cintura estatura, Test de salto largo, Test de abdominales, Test de flexo-extensiones de brazo, Test de Cafra y Test de Navette.Resultados: s e observó que los escolares con sobrepeso y obesidad (48,3%) mostraron un incremento significativo en las medidas antropométricas, así como una disminución en todas las variables que determinan rendimiento físico. En el análisis de los Test de Cafra y Navette, se observó que la presencia de la alteración de al menos uno de ellos se asoció con riesgo cardiometabólico (OR= 9,29, pobesidad (OR = 11,8, p = 0,003) y con riesgo cardiometabólico (OR = 30, 1, pobesidad presentaron disminución en todas las variables que determinaron el rendimiento físico y fue la alteración capacidad cardiorrespiratoria medida a través de los Test de Cafra y Navette los que presentaron mayor asociación y significativa con el sobrepeso, obesidad y riesgo cardiometabólico.

  2. [Not available].

    Science.gov (United States)

    López-Sobaler, Ana M; Aparicio Vizuete, Aránzazu; Ortega Anta, Rosa María

    2016-07-12

    Los kiwis, tanto la variedad verde (Actinidia deliciosa) como la de color amarillo (Sungold®) (A. chinensis), destacan entre las frutas de consumo habitual por su composición nutricional. Son excepcionalmente ricos en vitamina C, ya que los kiwis verdes duplican la cantidad que tienen fresas o naranjas, y el kiwi de color amarillo incluso la triplica. Además, los kiwis tienen un elevado contenido en vitaminas E, K, folatos, carotenoides, potasio, fibra y otros fitoquímicos, que proporcionan no solo beneficios nutricionales, sino también sanitarios. El consumo regular de kiwi en el contexto de una dieta equilibrada tiene efectos beneficiosos sobre la función inmune y defensa antioxidante; en la función gastrointestinal, mejorando la digestión proteica y el estreñimiento; y en el tracto respiratorio superior, ayudando en la prevención de infecciones y mejorando su sintomatología. Por último, el consumo habitual de kiwi se ha asociado a mejoras del estado de ánimo. La mayoría de estos beneficios pueden deberse al elevado contenido de vitamina C del kiwi, pero también a los otros nutrientes y fitoquímicos que actúan de forma sinérgica en la matriz alimentaria. Los resultados de los estudios realizados hasta ahora sugieren que el consumo diario de kiwi puede ser una estrategia efectiva para la promoción de la salud y prevención de numerosas enfermedades.

  3. [Not Available].

    Science.gov (United States)

    Burgos Peláez, Rosa; Cuerda Compes, María Cristina; García-Luna, Pedro P; Martínez Faedo, Ceferino; Mauri Roca, Sílvia; Moreno Villares, José Manuel; Virgili Casas, M Nuria; Wanden-Berghe, Carmina

    2016-07-19

    Introducción:la nutrición parenteral (NP) a largo plazo puede asociarse a complicaciones graves, con un deterioro importante de la calidad de vida de los pacientes con síndrome de intestino corto (SIC). Teduglutida, un análogo del péptido-2 similar al glucagón, pertenece a una nueva familia terapéutica y representa el primer abordaje no sintomático del SIC. Objetivos: revisar los datos preclínicos y clínicos en cuanto a eficacia y seguridad de teduglutida. Resultados: la aprobación de teduglutida se basó en los resultados de un estudio en fase III de 24 semanas, doble ciego, controlado con placebo (STEPS). Pacientes con fallo intestinal por SIC dependientes de NP ≥ 3 veces/semana durante ≥ 12 meses recibieron 0,05 mg/kg de teduglutida (n = 43) o placebo (n = 43) 1 vez/día. En la semana 24 hubo significativamente más respondedores en el grupo de teduglutida que en el de placebo (63 vs.30%; p = 0,002). La reducción absoluta media del volumen de NP frente al valor basal en la semana 24 fue significativamente mayor con teduglutida (4,4 vs.2,3 l/semana; p tratados con teduglutida vs.23% con placebo. Del total de pacientes que recibieron teduglutida en los ensayos en fase III (n = 134), el 12% consiguió una autonomía completa de la NP. Por lo general, la administración subcutánea de teduglutida se toleró bien. Conclusiones: se ha demostrado que teduglutida recupera la absorción intestinal y reduce significativamente la dependencia de la NP, consiguiendo incluso la independencia en algunos pacientes.

  4. [Not Available].

    Science.gov (United States)

    Ballesteros-Pomar, María; Villar-Taibo, Rocío; Calleja-Fernández, Alicia; Pintor-de-la-Maza, Begoña; Álvarez-Del-Campo, Cecilia; Vidal-Casariego, Alfonso; Cano-Rodríguez, Isidoro

    2016-06-03

    Los datos del estudio PREDYCES® nos revelaron que en España la desnutrición relacionada con la enfermedad (DRE) afecta a uno de cada cuatro pacientes hospitalizados. Esta cifra aumenta hasta el 36,8% en los pacientes hematológicos. Se calcula que un 20% de los pacientes oncológicos muere por complicaciones relacionadas con la DRE. Nuestro grupo se planteó en 2011 comenzar la implantación de un cribado nutricional en los servicios con mayor riesgo de DRE. La presente revisión trata de describir todo el proceso que hemos seguido para mejorar la situación nutricional en los pacientes ingresados en el Servicio de Hematología del Complejo Asistencial Universitario de León (CAULE), mayoritariamente con diagnóstico de neoplasias hematológicas. En un primer estudio piloto, detectamos una alta prevalencia de desnutrición, que tendió a aumentar durante la hospitalización. Además, solo el 8,3% los enfermos valorados recibieron algún tipo de soporte nutricional y no se estaban cubriendo sus necesidades ni calóricas ni proteicas, lo que se asociaba a un peor pronóstico. Por este motivo, nos decidimos a implantar de manera sistemática un cribado y una intervención nutricional adecuada, que comenzó en 2011 y que ha recibido el reconocimiento como Buena Práctica del Sistema Nacional de Salud.

  5. [Not Available].

    Science.gov (United States)

    Visiedo, Andrea; Sainz de Baranda, Pilar; Crone, Diane; Aznar, Susana; Pérez-Llamas, Francisca; Sánchez-Jiménez, Regina; Velázquez, Francisca; Berná-Serna, Juan de Dios; Zamora, Salvador

    2016-07-19

    Introducción y objetivos: en el presente trabajo se ha realizado una revisión sistemática durante los últimos 15 años de los estudios científicos que se han desarrollado con el objetivo de aumentar los niveles de actividad física, mejorar la salud y disminuir la prevalencia de sobrepeso y obesidad en escolares de entre 5 y 10 años.Material y métodos: se han encontrado un total de 28 estudios: 2 revisiones sistemáticas, 2 artículos descriptivos del proceso metodológico y 24 de intervención. Se pueden diferenciar dos tipos de intervenciones, por un lado aquellas intervenciones centradas en aumentar los niveles de actividad física, y por otro, aquellas que introducen además una parte de educación y valoración nutricional.Resultados: el análisis de los resultados muestra mejoras estadísticamente significativas en el 47,3% de las intervenciones que evaluaron el IMC, en el 44,4% de las que evaluaron la composición corporal, en el 40% de las que evaluaron el índice cintura-cadera, en el 50% de las que evaluaron el sumatorio de pliegues cutáneos. En relación con los efectos sobre la condición física, destacar que en el 45,4% de las intervenciones que avaluaron la resistencia cardiovascular se observaron mejoras significativas, al igual que en el 66,6% de las que analizaron los efectos sobre la fuerza. Finalmente, se observaron cambios en los hábitos alimentarios o en el conocimiento de los escolares en alimentación y nutrición en el 66,6% de los estudios que evaluaron dichos parámetros.

  6. [Not Available].

    Science.gov (United States)

    Domínguez, Raúl; Garnacho-Castaño, Manuel Vicente; Maté-Muñoz, José Luis

    2016-06-30

    La fuerza influye directamente en el estado de salud y en la capacidad de fitness, motivo por el que el entrenamiento contra resistencias o resistance training (RT) se incluye dentro de aquellos programas de ejercicio encaminados a mejorar la salud y calidad de vida. Debido a que muchasenfermedades cursan con alteración de la masa y funcionalidad muscular y a que el RT es la principal modalidad de ejercicio encaminada a mejorar la función muscular, el objetivo de la presente revisión bibliográfica ha sido exponer las evidencias actuales sobre las adaptaciones delRT, así como su posible aplicación en patologías como la obesidad, diabetes, dislipemia, hipertensión, cáncer, Parkinson, esclerosis múltiple o fibromialgia. El RT en estas enfermedades puede aumentar los niveles de masa muscular, disminuyendo los niveles de masa grasa, los nivelesde ácidos grasos en sangre y la glucemia, incrementando la sensibilidad a la insulina, y disminuyendo los niveles de citokinas inflamatorias. El RT, además, mejora el gasto cardiaco y la funcionalidad endotelial, regulando la tensión arterial e incrementando el consumo de oxígeno. Las ganancias de fuerza muscular mejoran la funcionalidad y la calidad de vida, especialmente en población con una afectación neuromuscular grave, como pudieran ser los enfermos de esclerosis múltiple, fibromialgia o Parkinson. Por ello, el RT debe ser incorporado como parte del tratamiento en las personas que presentan determinado tipo de patologías.

  7. Registration of two confection sunflower germplasm Lines, HA-R10 and HA-R11, Resistant to sunflower rust

    Science.gov (United States)

    Two confection sunflower (Helianthus annuus L.) germplasm lines, HA-R10 (Reg. No.xxx, PI670043) and HA-R11 (Reg. No.xxx, PI670044) were developed by the USDA-ARS Sunflower and Plant Biology Research Unit in collaboration with the North Dakota Agricultural Experiment Station and released December, 20...

  8. Registration of two double rust resistant germplasms, HA-R12 and HA-R13 for confection sunflower

    Science.gov (United States)

    The confection sunflower (Helianthus annuus L.) germplasms HA-R12 (Reg. No. ______, PI 673104) and HA-R13 (Reg. No. ______, PI 673105) were developed by the USDA-ARS, Sunflower and Plant Biology Research Unit in collaboration with the North Dakota Agricultural Experiment Station, and released in Jul...

  9. Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes

    Directory of Open Access Journals (Sweden)

    Mattiuz Pier

    2005-10-01

    Full Text Available Abstract Background The minor histocompatibility antigens (mHags are self-peptides derived from common cellular proteins and presented by MHC class I and II molecules. Disparities in mHags are a potential risk for the development of graft-versus-host disease (GvHD in the recipients of bone marrow from HLA-identical donors. Two alleles have been identified in the mHag HA-1. The correlation between mismatches of the mHag HA-1 and GvHD has been suggested and methods to facilitate large-scale testing were afterwards developed. Methods We used sequence specific primer (SSP PCR and direct sequencing to detect HA-1 gene polymorphisms in a sample of 131 unrelated Italian subjects. We then set up a novel melting temperature (Tm assay that may help identification of HA-1 alleles without oligonucleotide probes. Results We report the frequencies of HA-1 alleles in the Italian population and the presence of an intronic 5 base-pair deletion associated with the immunogeneic allele HA-1H. We also detected novel variable sites with respect to the consensus sequence of HA-1 locus. Even though recombination/gene conversion events are documented, there is considerable linkage disequilibrium in the data. The gametic associations between HA-1R/H alleles and the intronic 5-bp ins/del polymorphism prompted us to try the Tm analysis with SYBR® Green I. We show that the addition of dimethylsulfoxide (DMSO during the assay yields distinct patterns when amplicons from HA-1H homozygotes, HA-1R homozygotes, and heterozygotes are analysed. Conclusion The possibility to use SYBR® Green I to detect Tm differences between allelic variants is attractive but requires great caution. We succeeded in allele discrimination of the HA-1 locus using a relatively short (101 bp amplicon, only in the presence of DMSO. We believe that, at least in certain assets, Tm assays may benefit by the addition of DMSO or other agents affecting DNA strand conformation and stability.

  10. Sphagnum-dominated bog systems are highly effective yet variable sources of bio-available iron to marine waters.

    Science.gov (United States)

    Krachler, Regina; Krachler, Rudolf F; Wallner, Gabriele; Steier, Peter; El Abiead, Yasin; Wiesinger, Hubert; Jirsa, Franz; Keppler, Bernhard K

    2016-06-15

    Iron is a micronutrient of particular interest as low levels of iron limit primary production of phytoplankton and carbon fluxes in extended regions of the world's oceans. Sphagnum-peatland runoff is extraordinarily rich in dissolved humic-bound iron. Given that several of the world's largest wetlands are Sphagnum-dominated peatlands, this ecosystem type may serve as one of the major sources of iron to the ocean. Here, we studied five near-coastal creeks in North Scotland using freshwater/seawater mixing experiments of natural creek water and synthetic seawater based on a (59)Fe radiotracer technique combined with isotopic characterization of dissolved organic carbon by Accelerator Mass Spectrometry. Three of the creeks meander through healthy Sphagnum-dominated peat bogs and the two others through modified peatlands which have been subject to artificial drainage for centuries. The results revealed that, at the time of sampling (August 16-24, 2014), the creeks that run through modified peatlands delivered 11-15μg iron per liter creek water to seawater, whereas the creeks that run through intact peatlands delivered 350-470μg iron per liter creek water to seawater. To find out whether this humic-bound iron is bio-available to marine algae, we performed algal growth tests using the unicellular flagellated marine prymnesiophyte Diacronema lutheri and the unicellular marine green alga Chlorella salina, respectively. In both cases, the riverine humic material provided a highly bio-available source of iron to the marine algae. These results add a new item to the list of ecosystem services of Sphagnum-peatlands.

  11. Formation of a solar Ha filament from orphan penumbrae

    CERN Document Server

    Buehler, D; van Noort, M; Solanki, S K

    2016-01-01

    The formation of an Ha filament in active region (AR) 10953 is described. Observations from the Solar Optical Telescope (SOT) aboard the Hinode satellite starting on 27th April 2007 until 1st May 2007 were analysed. 20 scans of the 6302A Fe I line pair recorded by SOT/SP were inverted using the SPINOR code. The inversions were analysed together with SOT/BFI G-band and Ca II H and SOT/NFI Ha observations. Following the disappearance of an initial Ha filament aligned along the polarity inversion line (PIL) of the AR, a new Ha filament formed in its place some 20 hours later, which remained stable for at least 1.5 days. The creation of the new Ha filament was driven by the ascent of horizontal magnetic fields from the photosphere into the chromosphere at three separate locations along the PIL. The magnetic fields at two of these locations were situated directly underneath the initial Ha filament and formed orphan penumbrae already aligned along the Ha filament channel. The 700 G orphan penumbrae were stable and ...

  12. Sphagnum-dominated bog systems are highly effective yet variable sources of bio-available iron to marine waters

    Energy Technology Data Exchange (ETDEWEB)

    Krachler, Regina, E-mail: regina.krachler@univie.ac.at [Institute of Inorganic Chemistry, University of Vienna, Währingerstraße 42, 1090 Vienna (Austria); Krachler, Rudolf F.; Wallner, Gabriele [Institute of Inorganic Chemistry, University of Vienna, Währingerstraße 42, 1090 Vienna (Austria); Steier, Peter [Isotope Research and Nuclear Physics, University of Vienna, Währingerstraße 17, 1090 Vienna (Austria); El Abiead, Yasin; Wiesinger, Hubert [Institute of Inorganic Chemistry, University of Vienna, Währingerstraße 42, 1090 Vienna (Austria); Jirsa, Franz [Institute of Inorganic Chemistry, University of Vienna, Währingerstraße 42, 1090 Vienna (Austria); University of Johannesburg, Department of Zoology, P. O. Box 524, Auckland Park 2006 (South Africa); Keppler, Bernhard K. [Institute of Inorganic Chemistry, University of Vienna, Währingerstraße 42, 1090 Vienna (Austria)

    2016-06-15

    Iron is a micronutrient of particular interest as low levels of iron limit primary production of phytoplankton and carbon fluxes in extended regions of the world's oceans. Sphagnum-peatland runoff is extraordinarily rich in dissolved humic-bound iron. Given that several of the world's largest wetlands are Sphagnum-dominated peatlands, this ecosystem type may serve as one of the major sources of iron to the ocean. Here, we studied five near-coastal creeks in North Scotland using freshwater/seawater mixing experiments of natural creek water and synthetic seawater based on a {sup 59}Fe radiotracer technique combined with isotopic characterization of dissolved organic carbon by Accelerator Mass Spectrometry. Three of the creeks meander through healthy Sphagnum-dominated peat bogs and the two others through modified peatlands which have been subject to artificial drainage for centuries. The results revealed that, at the time of sampling (August 16–24, 2014), the creeks that run through modified peatlands delivered 11–15 μg iron per liter creek water to seawater, whereas the creeks that run through intact peatlands delivered 350–470 μg iron per liter creek water to seawater. To find out whether this humic-bound iron is bio-available to marine algae, we performed algal growth tests using the unicellular flagellated marine prymnesiophyte Diacronema lutheri and the unicellular marine green alga Chlorella salina, respectively. In both cases, the riverine humic material provided a highly bio-available source of iron to the marine algae. These results add a new item to the list of ecosystem services of Sphagnum-peatlands. - Highlights: • We report that peat-bogs are sources of bio-available iron to marine algae. • This iron is effectively chelated with aquatic humic acids. • The radiocarbon age of the iron-carrying aquatic humic acids was up to 550 years. • Analysis was focused on mixing experiments of iron-rich creek water with seawater. • Drained

  13. Magneto-optical and catalytic properties of Fe3O4@HA@Ag magnetic nanocomposite

    Science.gov (United States)

    Amir, Md.; Güner, S.; Yıldız, A.; Baykal, A.

    2017-01-01

    Fe3O4@HA@Ag magnetic nanocomposites (MNCs) were successfully synthesized by the simple reflux method for the removal of azo dyes from the industrial aqueous media. Fe3O4@HA@AgMNCs exhibited high catalytic activity to reduce MB within 20 min from the waste water. The obtained materials were characterized by the means of different techniques. Powder X-ray diffraction (XRD) analysis confirmed the single-phase of Fe3O4 spinel structure. SEM and TEM analysis indicated that Fe3O4@HA@AgMNCs were nanoparticles like structure with small agglomeration. TG result showed that the products contained 9% of HA. The characteristic peaks of HA at 1601 cm-1 and 1703 cm-1 was observed by the means of FT-IR spectra of Fe3O4@HA@AgMNCs. The hysteresis (σ-H) curves revealed Fe3O4@HA@Ag MNCs exhibit a typical superparamagnetic characteristic with a saturation magnetization of 59.11 emu/g and measured magnetic moment is 2.45 μB. The average magnetic particle dimension (Dmag) is 13.25 nm. In accordance, the average crystallite and particle dimensions were obtained as 11.50 nm and 13.10 nm from XRD and TEM measurements, respectively. Magnetocrystalline anisotropy was offered as uniaxial and calculated effective anisotropy constant (Keff) is 2.96×105 Erg/g. The blocking temperature was estimated as 522 K. The size-dependent saturation magnetization suggests the existence of a magnetically dead layer as 0.793 nm for Fe3O4@HA@Ag MNCs. The UV-vis diffuse reflectance spectroscopy (DRS) and Kubelka-Munk theory were applied to determine the optical properties of powder samples. The direct optical energy band gap (Eg) values were estimated from Tauc plots between 1.62 eV and 2.12 eV.

  14. [Not Available].

    Science.gov (United States)

    Perales-García, Aránzazu; Estévez-Martínez, Isabel; Urrialde, Rafael

    2016-07-12

    Introducción: la hidratación se define como el aporte de agua proveniente de alimentos y bebidas. Su estudio constituye desde hace unos años un área en sí misma dentro de la nutrición, que ha supuesto que en 2010 la European Food Safety Authority (EFSA) aprobara las recomendaciones de ingesta de agua; pero el estudio del estado de hidratación implica un elevado nivel metodológico que presenta ciertos desafíos. Objetivo: ofrecer una visión general de los principales problemas metodológicos en estudios sobre el estado de hidratación. Material y métodos: revisión de la bibliografía científica existente. Resultados: los principales problemas metodológicos presentes son: selección de la muestra (ámbito de investigación y diseño muestral), selección del método de evaluación del estado de hidratación (técnicas de dilución, impedancia bioeléctrica, indicadores plasmáticos y urinarios, cambios en la composición corporal, pérdidas de agua y síntomas clínicos), selección del método de valoración de la ingesta de agua (biomarcadores, cuestionarios, programas informáticos, uso de smartphones, registro 24 h, historia dietética y frecuencia de consumo de alimentos), así como sus principales fuentes dietéticas. Conclusiones: el estado de hidratación debe ser entendido como un modelo rutinario, de frecuencia diaria y variable en función de la edad, sexo, actividad física y condiciones ambientales; por todo ello es de especial importancia un correcto diseño metodológico capaz de recoger estos matices.

  15. [Not Available].

    Science.gov (United States)

    Peñailillo Escarate, Luis; Mackay Phillips, Karen; Serrano Duarte, Natalia; Canales Espinoza, Pablo; Miranda Herrera, Pamela; Zbinden-Foncea, Hermann

    2016-07-19

    Introducción: el entrenamiento de intervalos de alta intensidad (HIIT) y el consumo de ácidos grasos omega-3 (O3) ha demostrado cada uno por separado aumentar la capacidad aeróbica, metabolismo oxidativo y función cardiovascular.Objetivo: examinar el efecto combinado de HIIT más suplementación de O3 en el rendimiento físico, presión arterial y composición corporal en jóvenes sedentarios.Método: 28 jóvenes sedentarios con sobrepeso (Edad=22 ± 4 años; IMC=25.8 ± 2.4 kg·m-2) fueron distribuidos aleatoriamente en cuatro grupos: grupo O3/HIIT (n=7) realizó un protocolo de HIIT, tres veces por semana durante seis semanas y consumió 2 g·día-1 de O3; grupo HIIT (n=7) realizó solo el HIIT; grupo O3 (n=7) solo consumió O3; y grupo CONTROL (n=7) que no realizó ninguna intervención. Consumo de oxígeno peak (VO2peak), velocidad máxima (Vmax), presión arterial sistólica y diastólica (PAS y PAD), y porcentaje de grasa fueron medidos antes y después de la intervención.Resultados: el consumo de oxígeno peak aumentó más en el grupo O3/HIIT (+10.9%) en comparación con HIIT, O3 y CONTROL. Velocidad máxima aumentó en O3/HIIT (+7.1%) y HIIT (+11.9%). La presión arterial sistólica disminuyó más en O3 (-6.8%) en comparación con O3/HIIT, HIIT y CONTROL. Por último, O3/HIIT (-19.2%), HIIT (-20.2%), y O3 (-15.2%) presentaron mayores disminuciones del porcentaje de masa grasa en relación al CONTROL.Conclusión: nuestros resultados sugieren un efecto potenciador de la capacidad aeróbica máxima producto de la combinación de HIIT y suplementación de O3. Además, se observó una disminución de masa grasa en todos los grupos intervenidos.

  16. [Not Available].

    Science.gov (United States)

    De Arriba Muñoz, Antonio; López Úbeda, Marta; Rueda Caballero, Carmen; Labarta Aizpún, José Ignacio; Ferrández Longás, Ángel

    2016-07-19

    Introducción: saber diagnosticar y tratar la obesidad se ha convertido en el mayor reto del siglo XXI, debido al aumento de su prevalencia.Objetivos: determinar los valores de normalidad de perímetro abdominal (PA) e índice de masa corporal (IMC) según edad y sexo en población española sana.Métodos: estudio longitudinal observacional realizado entre 1980 y 2014. Se incluyeron 165 niños y 169 niñas recién nacidas, recogiendo datos de forma anual hasta los 18 años (74 varones y 92 mujeres), y posteriormente a los 28 años (42 varones y 45 mujeres). Se realizó medición de peso, longitud/talla y perímetro abdominal. Se calcularon los percentiles (P3, P10, P25, P50, P75, P90, P97) de IMC y PA según edad y sexo.Resultados: se presentan datos evolutivos de IMC y PA durante la infancia, destacando cómo aumentan los valores entre los 18 y 28 años de los percentiles superiores al p50, sobre todo en mujeres. Existe una correlación positiva en relación al PA entre el valor obtenido a los 3 años con el valor de los 18 años y de los 28 años tanto en varones (r = 0,722 y r = 0,605, p = 0,000, respectivamente) como en mujeres(r = 0,922, r = 0,857, p = 0,000, respectivamente). Y entre los 18 y 28 años (r = 0,731, p = 0,000 para varones y r = 0,961, p = 0,000 para mujeres).Conclusión: se presentan valores de normalidad de PA e IMC según edad y sexo, que podrán utilizarse como herramienta de referencia para identificar a personas con riesgo de desarrollar enfermedades cardiovasculares o diabetes.

  17. [Not Available].

    Science.gov (United States)

    MIján de la Torre, Alberto

    2016-06-03

    El síndrome de caquexia cancerosa es responsable de la muerte de un número significativo de pacientes con cáncer. Se caracteriza por la presencia de una ingesta reducida, con inflamación sistémica y un metabolismo alterado. Los enfermos presentan característicamente una progresiva pérdida de peso y de masa muscular, junto a deterioro funcional. La pérdida muscular se debe a la combinación de reducción de la síntesis proteica con aumento de su degradación. Ello conduce tanto a un acortamiento como a una reducción en el área de la fibra muscular. Asimismo, existen datos que apoyan que selectivamente algunos de los tipos de fibra muscular se ven más afectados. Es necesario definir bien los valores de corte de sarcopenia para diagnosticar la pérdida muscular y existen diferentes métodos. El sistema de la ubiquitina-proteasoma parece desempeñar un papel predominante en la degradación de la proteína miofibrilar. La tendencia a perder masa muscular en los pacientes con caquexia cancerosa parece estar asociada a la activación de señales catabólicas por citoquinas proinflamatorias, así como por productos tumorales del tipo factor inductor de proteólisis. En referencia a los factores pronósticos, el riesgo de muerte está bien documentado en pacientes con sarcopenia y, especialmente, en aquellos con obesidad asociada a la sarcopenia. Asimismo, se ha establecido una relación directa entre la pérdida intensa de masa muscular y la supervivencia en pacientes con diferentes tipos de tumores del tipo de cáncer de páncreas, pulmón, tracto biliar o cáncer colorrectal. Respecto de la terapia en el síndrome de caquexia cancerosa, es factible que requiera tratamiento con varios grupos combinados que incluyan, junto al soporte nutricional, fármacos orexígenos, con efecto anabólico y antinflamatorio, asociados a intervenciones que estimulen el ejercicio físico.

  18. [Not Available].

    Science.gov (United States)

    Germán Díaz, Marta; Moreno Villares, José Manuel; Gomis Muñoz, Pilar

    2016-07-19

    Introducción: la nutrición parenteral domiciliaria se ha convertido en un punto clave en el tratamiento de pacientes con fracaso intestinal crónico. A pesar de los importantes avances que se han producido en las últimas décadas, tanto en los accesos vasculares, como en las soluciones empleadas, las infecciones asociadas a catéter venoso central siguen constituyendo una de las complicaciones más importantes. Dentro de las estrategias para la prevención o el tratamiento de estas infecciones se encuentra el empleo de sellados con antisépticos, como el etanol o la taurolidina, o de antibióticos.Objetivo: el objetivo de este artículo es revisar la evidencia disponible sobre el empleo de sellados con antisépticos o antibióticos en el manejo de pacientes pediátricos con nutrición parenteral domiciliaria.Material y métodos: el uso de sellados con etanol o taurolidina para prevenir el desarrollo de infecciones asociadas a catéter central estaría indicado en pacientes con nutrición parenteral domiciliaria que hayan tenido más de una infección en el año anterior o que se consideren pacientes de riesgo. Los sellados con antibióticos están indicados en el tratamiento de bacteriemias asociadas a catéter central producidas por S. coagulasa-negativo o gramnegativos, asociados a un tratamiento sistémico, siempre que sea posible, con el fin de salvar el catéter. Se debería llevar a cabo la retirada del mismo cuando existan signos de infección del punto de entrada o del trayecto subcutáneo, o cuando el germen responsable de la infección sea S. aureus o Cándida.Conclusión: a pesar de que la fuerza de la evidencia sobre la eficacia del sellado en la prevención o el tratamiento de infecciones asociadas al catéter es limitada, tanto en el niño como en el adulto, cada vez existen más datos a usar esta alternativa en pacientes con nutrición parenteral domiciliaria en los que la atención y salvaguarda de los catéteres es primordial.

  19. Confusional State in HaNDL Syndrome: Case Report and Literature Review

    Directory of Open Access Journals (Sweden)

    Sarah Nelson

    2013-01-01

    Full Text Available The syndrome of transient headache and neurologic deficits with cerebrospinal fluid lymphocytosis (HaNDL syndrome is a self-limited condition. Confusional states are uncommonly reported as a clinical manifestation of this syndrome. Here, I report a 76-year-old female who presented with headache, confusion, and agitation with a mild CSF lymphocytosis. Other workup to determine the cause of her altered mental status was otherwise negative. The literature available in the English language on HaNDL syndrome is reviewed, including its history, pathophysiology, possible associations with migraine and stroke, and previously reported cases of confusional states in this syndrome. While HaNDL syndrome has been a described entity since the 1980s, its pathophysiology has yet to be clearly defined.

  20. NOAA Operational Model Archive Distribution System (NOMADS): High Availability Applications for Reliable Real Time Access to Operational Model Data

    Science.gov (United States)

    Alpert, J. C.; Wang, J.

    2009-12-01

    To reduce the impact of natural hazards and environmental changes, the National Centers for Environmental Prediction (NCEP) provide first alert and a preferred partner for environmental prediction services, and represents a critical national resource to operational and research communities affected by climate, weather and water. NOMADS is now delivering high availability services as part of NOAA’s official real time data dissemination at its Web Operations Center (WOC) server. The WOC is a web service used by organizational units in and outside NOAA, and acts as a data repository where public information can be posted to a secure and scalable content server. A goal is to foster collaborations among the research and education communities, value added retailers, and public access for science and development efforts aimed at advancing modeling and GEO-related tasks. The user (client) executes what is efficient to execute on the client and the server efficiently provides format independent access services. Client applications can execute on the server, if it is desired, but the same program can be executed on the client side with no loss of efficiency. In this way this paradigm lends itself to aggregation servers that act as servers of servers listing, searching catalogs of holdings, data mining, and updating information from the metadata descriptions that enable collections of data in disparate places to be simultaneously accessed, with results processed on servers and clients to produce a needed answer. The services used to access the operational model data output are the Open-source Project for a Network Data Access Protocol (OPeNDAP), implemented with the Grid Analysis and Display System (GrADS) Data Server (GDS), and applications for slicing, dicing and area sub-setting the large matrix of real time model data holdings. This approach insures an efficient use of computer resources because users transmit/receive only the data necessary for their tasks including

  1. La Ha de la infancia en Italia. Problemas y perspectiva

    Directory of Open Access Journals (Sweden)

    Enzo CATARSI

    2010-03-01

    Full Text Available RESUMEN: La historiografía italiana de la infancia, como la de otros muchos países europeos, está hoy embarcada en una tarea de desarrollo y profundización que permitirá recuperar los retrasos que la han caracterizado. En efecto, también en Italia la historia de la infancia y su individuación como «objeto» historiografía constituyen una adquisición reciente, lograda con la ayuda de diversas influencias. La primera, estrechamente vinculada a la historia de las mentalidades, representada por la obra de Ph. Aries, «El niño y la vida familiar en el Antiguo Régimen», traducido al italiano en 1968. La segunda es la historia social, orientada al estudio de los diversos aspectos de la vida de una sociedad, y más atenta a sus acontecimientos. La tercera la representa el estructuralismo, especialmente el francés, y en concreto M. Foucault. La particular influencia que, también en el contexto italiano, han alcanzado los trabajos de Ph. Aries y L. DeMause, muy importantes por haber abierto una línea de investigación, aunque con las obligadas diferencias, ha conducido a privilegiar una perspectiva exclusiva de historia de las mentalidades que se muestra muy ambigua y claramente interpretativa en exceso.

  2. Haïti : entre urgence et reconstruction

    Directory of Open Access Journals (Sweden)

    Jean-Marc Biquet

    2014-01-01

    Full Text Available Note de l’éditeur : Cette contribution vient alimenter la section « Policy Debate » de la Revue de politique internationale de développement. Des universitaires, décideurs et praticiens y dialoguent autour d’enjeux globaux du développement. Le processus de publication des « Policy Debate » est différent de celle des autres sections. Les contributions sont éditées et corrigées, mais pas expertisés par des pairs. Une contribution thématique initiale lance le débat. Elle est suivie de commentaires critiques et de réactions de diverses parties. Jean-Marc Biquet, chargé de recherche à Médecin sans Frontière (MSF, livre une contribution sur la faillite du système d’aide en Haïti. Ce pays a été particulièrement touché par deux catastrophes coup sur coup : le séisme en janvier 2010 et une épidémie de choléra en octobre de la même année. Malgré des demandes répétées (et engagements reçus aux Nations unies (en particulier, à l’UNOCHA, nous n’avons malheureusement pas reçu de réaction écrite à l’article de Jean-Marc Biquet provenant de ces institutions. Cependant, nous avons reçu un commentaire critique de Andrea Binder, Directrice associée du Global Public Policy Institute (GPPi de Berlin ‘Is the Humanitarian Failure in Haiti a System Failure?’, que nous publions conjointement au texte de Jean-Marc Biquet. Dr Biner a notamment été étroitement impliquée dans le processus  d’évaluation inter-agences en temps réel de l’intervention humanitaire en Haïti suite au tremblement de terre. Téléchargez l’intégralité du débat en format .pdf sur http://poldev.revues.org/pdf/1600

  3. Electrochemical Properties of HA Coated Titanium Dioxide Nanotubes.

    Science.gov (United States)

    Kang, Kyungho; Zakiyuddin, Ahmad; Lee, Kwangmin

    2016-02-01

    CP Grade II titanium was first anodized in order to form TiO2 nanotubes, and then the TiO2 nano- tubes were coated with hydroxyapatite using the sol-gel method. The electrochemical properties of pure Ti, anodized TiO2 nanotubes, and HA-coated TiO2 nanotubes were investigated using poten- tiodynamic polarization and impedance tests. The sol-gel-coated HA nanoparticles were observed to sufficiently penetrate into the TiO2 nanotubes, and the polarization resistance of the HA-coated titanium nanotubes was higher than that of those that had just been anodized. In conclusion, the HA coating on the TiO2 nanotubes provides improved electrochemical properties and can be effective in overcoming the negative influence of passive TiO2 films.

  4. Haïti et l'anthropologie

    OpenAIRE

    2008-01-01

    Le premier numéro de la nouvelle série de GRADHIVA est consacré à l’anthropologie en Haïti : ses origines, son rôle, ses évolutions récentes, son devenir. Ce numéro double de 272 pages, illustré de nombreux clichés, évoque les rapports étroits entre l’anthropologie et la culture multiforme d’Haïti, à travers l’oeuvre de peintres, d’historiens, d’écrivains, ou d’ethnologues haïtiens. Le dossier analyse le développement d’une anthropologie haïtienne nécessairement liée à la question raciale et ...

  5. A-Ha. Drum'n'bassi supernimi FABIO

    Index Scriptorium Estoniae

    2002-01-01

    7.sept. annab norra menukaim popansambel A-Ha Tallinnas Lauluväljakul kontserdi, kus presenteerib ka oma viimast albumit "Lifelines". 14. sept.tuleb drum'n'bassi spetsialist Fabio Tallinna üritusele Circulation

  6. [Not Available].

    Science.gov (United States)

    1992-03-25

    Archersstar Sudha Bhuchar, right, launches a video on breast screening for women from ethnic minorities, sponsored by the NHS. The video is available in six languages. Ms Bhuchar is pictured with programme co-ordinator Julietta Patrick.

  7. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) C++ for Particle Physicists : 17 – 21.11.03 (6 X 3-hour lectures) Programmation automate Schneider TSX Premium – niveau 2 : 18 – 21.11.03 (4 jours) JAVA 2 Enterprise Edition – Part 1 : WEB Applications : 20 & ...

  8. Basonuclin regulates a subset of ribosomal RNA genes in HaCaT cells.

    Directory of Open Access Journals (Sweden)

    Shengliang Zhang

    Full Text Available Basonuclin (Bnc1, a cell-type-specific ribosomal RNA (rRNA gene regulator, is expressed mainly in keratinocytes of stratified epithelium and gametogenic cells of testis and ovary. Previously, basonuclin was shown in vitro to interact with rRNA gene (rDNA promoter at three highly conserved sites. Basonuclin's high affinity binding site overlaps with the binding site of a dedicated and ubiquitous Pol I transcription regulator, UBF, suggesting that their binding might interfere with each other if they bind to the same promoter. Knocking-down basonuclin in mouse oocytes eliminated approximately one quarter of RNA polymerase I (Pol I transcription foci, without affecting the BrU incorporation of the remaining ones, suggesting that basonuclin might regulate a subset of rDNA. Here we show, via chromatin immunoprecipitation (ChIP, that basonuclin is associated with rDNA promoters in HaCaT cells, a spontaneously established human keratinocyte line. Immunoprecipitation data suggest that basonuclin is in a complex that also contains the subunits of Pol I (RPA194, RPA116, but not UBF. Knocking-down basonuclin in HaCaT cells partially impairs the association of RPA194 to rDNA promoter, but not that of UBF. Basonuclin-deficiency also reduces the amount of 47S pre-rRNA, but this effect can be seen only after cell-proliferation related rRNA synthesis has subsided at a higher cell density. DNA sequence of basonuclin-bound rDNA promoters shows single nucleotide polymorphisms (SNPs that differ from those associated with UBF-bound promoters, suggesting that basonuclin and UBF interact with different subsets of promoters. In conclusion, our results demonstrate basonuclin's functional association with rDNA promoters and its interaction with Pol I in vivo. Our data also suggest that basonuclin-Pol I complex transcribes a subset of rDNA.

  9. Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Places available The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses : Introduction à Outlook : 19.8.2004 (1 journée) Outlook (short course I) : E-mail : 31.8.2004 (2 hours, morning) Outlook (short course II) : Calendar, Tasks and Notes : 31.8.2004 (2 hours, afternoon) Instructor-led WBTechT Study or Follow-up for Microsoft Applications : 7.9.2004 (morning) Outlook (short course III) : Meetings and Delegation : 7.9.2004 (2 hours, afternoon) Introduction ...

  10. Part II: crystalline fluorapatite-coated hydroxyapatite implant material: a dog study with histologic comparison of osteogenesis seen with FA-coated HA grafting material versus HA controls: potential bacteriostatic effect of fluoridated HA.

    Science.gov (United States)

    Nordquist, William D; Okudera, Hajima; Kitamura, Yutaka; Kimoto, Kazunari; Okudera, Toshimitsu; Krutchkoff, David J

    2011-01-01

    Success of osteogenesis in bone graft procedures can be enhanced by inhibiting oral bacterial infections through the use of prophylactic bacteriostatic fluoride within the grafting environment. Ideally, the fluoride ion should be chemically sequestered and thus unavailable unless needed at times during the process of early infection. As fluoride within fluorapatite is tightly bound at neutral pH and becomes available only during acidic conditions, fluorapatite is an ideal store for the fluoride ion which becomes released for bacteriostasis only during an acidic environment found with incipient bacterial infection. The purpose of this investigation was to compare the histologic properties of new bone formed surrounding fluorapatite (FA)-coated microcrystalline hydroxyapatite (HA) grafting material with comparable bone formed following the use of control HA material (OsteoGen, Impladent, Ltd, Holliswood, NY). The results of histologic analysis within dog studies here showed no detectable difference in new bone following therapeutic grafting procedures using each of the above 2 mineral coatings.

  11. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: C++ Programming Level 2 - Traps & Pitfalls:  16 - 19.7.02 (4 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch

  12. Cross-sectional analysis on microstructure of plasma-sprayed HA+TiO2 composite coatings on titanium substrate

    Institute of Scientific and Technical Information of China (English)

    吕宇鹏; 李木森; 王建华; 孙瑞雪; 李士同; 朱瑞富

    2004-01-01

    The cross-sectional analysis on hydroxyapatite (HA) coating and HA+TiO2 composite coating was conducted by using electron probe microanalyser (EPMA). The results reveal that annealing at 650 ℃ leads to the cracking within the HA coating or along the coating/substrate interface. The ribbon-like regions in HA coating are verified to contain less PO44- groups resulted from the high temperature melting of HA particles in plasma flame.From the viewpoint of microstructural observation, it can be concluded that the addition of TiO2 into HA coating can effectively strengthen and toughen the whole coating system with a shift of the well-bonded interface from the THA (top HA) coating/HTBC (HA+TiO2 bond coat) interface in the as-sprayed THBC (top HA-HTBC) coating to the HTBC/Ti substrate interface in the heat treated THBC coating. The THA coating bonds well to Ti substrate perhaps via its TiO2 hobnobbing with the Ti oxides formed on the Ti substrate.

  13. 77 FR 64183 - Notice of Availability of a Final General Conformity Determination for the California High-Speed...

    Science.gov (United States)

    2012-10-18

    ... California High-Speed Train System Merced to Fresno Section AGENCY: Federal Railroad Administration (FRA... Section of the California High-Speed Train (HST) System on September 18, 2012. FRA is the lead Federal... General Conformity requirements. The California High Speed Rail Authority (Authority), as the...

  14. Data of evolutionary structure change: 1B9HA-2FNIA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1B9HA-2FNIA 1B9H 2FNI A A -KAPEFPAWPQYDDAERNGLVRALEQGQWWRMGGDEVNS...------------QLFNTAPLKSAEDFYHAEISLPCHA-NLNLESVQNIAHSVLKTFESFKI - HHHHHHHHHHHH HHHHHHHHHHH EEEEE ... 2FNI A 2FNIA 2FNI A 2FNIA MLKKDFFEGEV 2FNI A 2FNIA KQLTQ--GKR

  15. Osteoblast interaction with laser cladded HA and SiO{sub 2}-HA coatings on Ti-6Al-4V

    Energy Technology Data Exchange (ETDEWEB)

    Yang Yuling [Department of Physics, Northeastern University, Shenyang 110004 (China); Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Serpersu, Kaan [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); He Wei, E-mail: whe5@utk.edu [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Department of Mechanical, Aerospace and Biomedical Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Paital, Sameer R. [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Dahotre, Narendra B. [Department of Materials Science and Engineering, University of North Texas, Denton, TX 76207 (United States)

    2011-12-01

    In order to improve the bioactivity and biocompatibility of titanium endosseous implants, the morphology and composition of the surfaces were modified. Polished Ti-6Al-4V substrates were coated by a laser cladding process with different precursors: 100 wt.% HA and 25 wt.% SiO{sub 2}-HA. X-ray diffraction of the laser processed samples showed the presence of CaTiO{sub 3}, Ca{sub 3}(PO{sub 4}){sub 2}, and Ca{sub 2}SiO{sub 4} phases within the coatings. From in vitro studies, it was observed that compared to the unmodified substrate all laser cladded samples presented improved cellular interactions and bioactivity. The samples processed with 25 wt.% SiO{sub 2}-HA precursor showed a significantly higher HA precipitation after immersion in simulated body fluid than 100 wt.% HA precursor and titanium substrates. The in vitro biocompatibility of the laser cladded coatings and titanium substrate was investigated by culturing of mouse MC3T3-E1 pre-osteoblast cell line and analyzing the cell viability, cell proliferation, and cell morphology. A significantly higher cell attachment and proliferation rate were observed for both laser cladded 100 wt.% HA and 25 wt.% SiO{sub 2}-HA samples. Compared to 100 wt.% HA sample, 25 wt.% SiO{sub 2}-HA samples presented a slightly improved cellular interaction due to the addition of SiO{sub 2}. The staining of the actin filaments showed that the laser cladded samples induced a normal cytoskeleton and well-developed focal adhesion contacts. Scanning electron microscopic image of the cell cultured samples revealed better cell attachment and spreading for 25 wt.% SiO{sub 2}-HA and 100 wt.% HA coatings than titanium substrate. These results suggest that the laser cladding process improves the bioactivity and biocompatibility of titanium. The observed biological improvements are mainly due to the coating induced changes in surface chemistry and surface morphology. Highlights: {yields} Laser cladding of Ti alloys with bioceramics creates new

  16. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval Tel. 74924technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: MATLAB Fundamentals and Programming Techniques (ML01) : 2 & 3.12.03 (2 days) Oracle 8i : SQL : 3 - 5.12.03 (3 days) The EDMS MTF in practice : 5.12.03 (afternoon, free of charge) Modeling Dynamic Systems with Simulink (SL01) : 8 & 9.12.03 (2 days) Signal Processing with MATLAB (SG01) : 11 & 12.12.03 (2 days) The JAVA Programming Language - l...

  17. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval Tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: JAVA 2 Enterprise Edition - Part 1 : WEB Applications : 20 & 21.11.03(2 days) FrontPage 2000 - niveau 1 : 20 & 21.11.03 (2 jours) Oracle 8i : SQL : 3 - 5.12.03 (3 days) Oracle 8i : Programming with PL/SQL : 8 - 10.12.03 (3 days) The JAVA Programming Language - leve...

  18. Places available**

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 :9 & 10.1.2004 (2 days) The JAVA Programming Language Level 2 : 11 to 13.1.2004 (3 days) Hands-on Introduction to Python Programming : 16 - 18.2.2004 (3 days - free of charge) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004...

  19. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses : EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) The EDMS-MTF in practice (free of charge) :  28 -  30.10.03 (6 half-day sessions) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) LabVIEW TestStand ver. 3 : 4 & 5.11.03 (2 days) Introduction to Pspice : 4.11.03 p.m. (half-day) Hands-on Introduction to Python Programm...

  20. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: MATLAB Fundamentals and Programming Techniques (ML01) :2 & 3.12.03 (2 days) Oracle 8i : SQL : 3 - 5.12.03 (3 days) The EDMS MTF in practice : 5.12.03 (afternoon, free of charge) Modeling Dynamic Systems with Simulink (SL01) : 8 & 9.12.03 (2 days) Signal Processing with MATLAB (SG01) : 11 & ...

  1. Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Project Planning with MS-Project : 15 & 22.1.2004 (2 days) Joint PVSS JCOP Framework Course : 2 sessions : 2 - 6.2.2004 and 16 - 20-2-2004 (5 days) Hands-on Introduction to Python Programming : 16 - 18.2.2004 (3 days - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 ( 6 X 4-hour sessions)

  2. Places available**

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 : 9 & 10.1.2004 (2 days) The JAVA Programming Language Level 2 : 11 to 13.1.2004 (3 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 ( 6 X 4-hour sessions) LabVIEW Basics 1 : 22 - 24.3.20...

  3. Next-generation phage display: integrating and comparing available molecular tools to enable cost-effective high-throughput analysis.

    Directory of Open Access Journals (Sweden)

    Emmanuel Dias-Neto

    Full Text Available BACKGROUND: Combinatorial phage display has been used in the last 20 years in the identification of protein-ligands and protein-protein interactions, uncovering relevant molecular recognition events. Rate-limiting steps of combinatorial phage display library selection are (i the counting of transducing units and (ii the sequencing of the encoded displayed ligands. Here, we adapted emerging genomic technologies to minimize such challenges. METHODOLOGY/PRINCIPAL FINDINGS: We gained efficiency by applying in tandem real-time PCR for rapid quantification to enable bacteria-free phage display library screening, and added phage DNA next-generation sequencing for large-scale ligand analysis, reporting a fully integrated set of high-throughput quantitative and analytical tools. The approach is far less labor-intensive and allows rigorous quantification; for medical applications, including selections in patients, it also represents an advance for quantitative distribution analysis and ligand identification of hundreds of thousands of targeted particles from patient-derived biopsy or autopsy in a longer timeframe post library administration. Additional advantages over current methods include increased sensitivity, less variability, enhanced linearity, scalability, and accuracy at much lower cost. Sequences obtained by qPhage plus pyrosequencing were similar to a dataset produced from conventional Sanger-sequenced transducing-units (TU, with no biases due to GC content, codon usage, and amino acid or peptide frequency. These tools allow phage display selection and ligand analysis at >1,000-fold faster rate, and reduce costs approximately 250-fold for generating 10(6 ligand sequences. CONCLUSIONS/SIGNIFICANCE: Our analyses demonstrates that whereas this approach correlates with the traditional colony-counting, it is also capable of a much larger sampling, allowing a faster, less expensive, more accurate and consistent analysis of phage enrichment. Overall

  4. IMPACT OF DYNAMICAL HYDRATION SHELL AROUND HA PROTEIN ON NONLINEAR CONCENTRATION DEPENDENT T-RAYS ABSORPTION

    Directory of Open Access Journals (Sweden)

    YIWEN SUN

    2013-10-01

    Full Text Available T-rays is sensitive to covalently cross-linked proteins and can be used to probe unique dynamic properties of water surrounding a protein. In this paper, we demonstrate the unique absorption properties of the dynamic hydration shells determined by hemagglutinin (HA protein in terahertz frequency. We study the changes arising from different concentrations in detail and show that nonlinear absorption coefficient is induced by the dynamic hydration water. The binary and ternary component model were used to interpret the nonlinearity absorption behaviors and predict the thickness of the hydration shells around the HA protein in aqueous phase.

  5. Trehalose improves cell proliferation and dehydration tolerance of human HaCaT cells

    Directory of Open Access Journals (Sweden)

    Lee Kyung Eun

    2015-01-01

    Full Text Available Trehalose is a disaccharide molecule that serves as a natural osmotic regulator in halophilic microorganisms and plants but not in mammals. We observed that human HaCaT cells supplied with trehalose improved cell proliferation and extended viability under dehydration. In HaCaT cells, in response to increasing concentrations of exogenous trehalose, the levels of heat shock protein (HSP 70 increased and matrix metalloproteinase (MMP 1 decreased. Proteome analysis of trehalose-treated HaCaT cells revealed remarkable increases in the levels of proteins involved in cell signaling and the cell cycle, including p21 activated kinase I, Sec I family domain protein and elongation factor G. Moreover, the proteins for cell stress resistance, tryptophan hydroxylase, serine/cysteine proteinase inhibitors and vitamin D receptors were also increased. In addition, the proteins responsible for the maintenance of the cytoskeleton and cellular structures including procollagen-lysine dioxygenase, vinculin and ezrin were increased. Proteomic data revealed that trehalose affected HaCaT cells by inducing the proteins involved in cell proliferation. These results suggest that trehalose improves the proliferation and dehydration tolerance of HaCaT cells by inducing proteins involved in cell growth and dehydration protection.

  6. HaNDL Syndrome Presenting During Pregnancy: A Case Report and Review of the Literature

    Directory of Open Access Journals (Sweden)

    Yüksel Kaplan

    2014-09-01

    Full Text Available Headache associated with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL is a self-limited syndrome characterized by sudden-onset headache with a temporary neurological deficit and cerebrospinal fluid (CSF lymphocytosis. We aimed to disscus a case of HaNDL syndrome presenting during pregnancy with relevant literature. A 20-year-old female presented with a 5-day history of severe, bilateral throbbing headache accompanied by nausea, vomiting, and phonophobia. Approximately 2 days after the pain developed, she became acutely confused for less than 90 minutes. 2 days after this episode, she experienced again confusional state and left hemiparesis. There were no symptoms consistent with meningoencephalitis. She was pregnant and at 11 weeks gestation. A neurologic examination showed confusional state, bilateral papilledema, and mild left hemiparesis. The neuroradiological examination was normal. The cerebrospinal fluid revealed lymphocytic pleocytosis, mildly elevated protein, and increased opening pressure. She recovered completely after 8 days. The precise etiology of HaNDL is unknown, although an inflammatory or infectious origin and autoimmune factors have been proposed. Moreover, the risk factors and medical conditions associated with HaNDL are unknown. It is obviously difficult to determine whether the pregnancy was coincidental or associated in this case. We believe that comprehensive studies are needed to clarify the risk factors and medical conditions associated with HaNDL.

  7. Possible detection of the stellar donor or remnant for the type Iax supernova 2008ha

    Energy Technology Data Exchange (ETDEWEB)

    Foley, Ryan J. [Astronomy Department, University of Illinois at Urbana-Champaign, 1002 West Green Street, Urbana, IL 61801 (United States); McCully, Curtis; Jha, Saurabh W. [Department of Physics and Astronomy, Rutgers, The State University of New Jersey, 136 Frelinghuysen Road, Piscataway, NJ 08854 (United States); Bildsten, Lars [Department of Physics, University of California, Santa Barbara, CA 93106 (United States); Fong, Wen-fai [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Narayan, Gautham [National Optical Astronomy Observatory, 950 North Cherry Avenue, Tucson, AZ 85719-4933 (United States); Rest, Armin [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States); Stritzinger, Maximilian D. [Department of Physics and Astronomy, Aarhus University, Ny Munkegade, DK-8000 Aarhus C (Denmark)

    2014-09-01

    Type Iax supernovae (SNe Iax) are thermonuclear explosions that are related to SNe Ia, but are physically distinct. The most important differences are that SNe Iax have significantly lower luminosity (1%-50% that of typical SNe Ia), lower ejecta mass (∼0.1-0.5 M {sub ☉}), and may leave a bound remnant. The most extreme SN Iax is SN 2008ha, which peaked at M{sub V} = –14.2 mag, about 5 mag below that of typical SNe Ia. Here, we present Hubble Space Telescope (HST) images of UGC 12682, the host galaxy of SN 2008ha, taken 4.1 yr after the peak brightness of SN 2008ha. In these deep, high-resolution images, we detect a source coincident (0.86 HST pixels; 0.''043; 1.1σ) with the position of SN 2008ha with M {sub F814W} = –5.4 mag. We determine that this source is unlikely to be a chance coincidence, but that scenario cannot be completely ruled out. If this source is directly related to SN 2008ha, it is either the luminous bound remnant of the progenitor white dwarf (WD) or its companion star. The source is consistent with being an evolved >3 M {sub ☉} initial mass star, and is significantly redder than the SN Iax 2012Z progenitor system, the first detected progenitor system for a thermonuclear SN. If this source is the companion star for SN 2008ha, there is a diversity in SN Iax progenitor systems, perhaps related to the diversity in SN Iax explosions. If the source is the bound remnant of the WD, it must have expanded significantly. Regardless of the nature of this source, we constrain the progenitor system of SN 2008ha to have an age of <80 Myr.

  8. Preparation and bioevaluation of 166Ho labelled hydroxyapatite (HA) particles for radiosynovectomy.

    Science.gov (United States)

    Unni, P R; Chaudhari, P R; Venkatesh, Meera; Ramamoorthy, N; Pillai, M R A

    2002-02-01

    The preparation of 166Ho labeled hydroxy apatite (HA) particles for radiosynovectomy applications is described in this paper. 166Ho was prepared by the irradiation of Ho2O3 at a flux of 1.8 x 10(13) neutrons/cm2/s for about 7 days. The irradiation resulted in the production of approximately 17 GBq of 166Ho activity at the end of six hours post end of bombardment and the corresponding specific activity was approximately 3-4 GBq/mg of Ho. The irradiated target was dissolved in 0.1 N HCl solution. Radionuclidic purity was ascertained by high resolution gamma ray spectrometry. HA particles were synthesized and characterized by X-ray diffractometry. Labeling studies were carried out with and without citric acid as a transchelating agent. Radiochemical yield and purity of the 166Ho-HA particles were ascertained by paper chromatography and by paper electrophoresis techniques. Labeling yield of >98% could be achieved at pH 7, with 40 mg of HA particles and 8.6 microg of Ho. 166Ho-HA particles prepared were stable for 72 h. Bio-evaluation of the 166Ho -HA particles were carried out by injecting approximately 74 MBq dose in 200 microL (approximately 8 mg of 166Ho-HA particles) directly into the arthritis induced knee joints as well as into the healthy knee joints of white New Zealand rabbits. Images of the injected joints of the animals recorded using a gamma camera at regular intervals showed good retention. Blood samples were collected from the animals and activity assayed in a scintillation detector. Experiments were also carried out under identical conditions in normal rabbits. In both the cases, it was observed that there was no significant extra articular leakage of the injected activity over the study period of 96 h post injection.

  9. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: C++ Programming Level 2 - Traps & Pitfalls:  16 - 19.7.02 (4 days) Frontpage 2000 - level 1 :  22 - 23.7.02  (2 days) Introduction à Windows 2000 au CERN : 24.7.02 (après-midi) CLEAN-2002 : Travailler en salle blanche (cours gratuit) : 13.08.2002 (matin) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch

  10. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: December 2002   PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) Introduction à la CAO Cadence (cours gratuit) :  10 & 11.12.02  (2 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch

  11. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: CLEAN-2002 : Travailler en salle blanche (cours gratuit) : 13.08.2002 (matin) Introduction to the CERN Enginnering Data Management System :  27.8.02  (1 day) The CERN Engineering Data Management System for Advanced Users :  28.8.02  (1 day) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval Tel.74924 monique.duval@cern.ch    

  12. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction to Databases :  3 - 4.7.01 (2 days) The JAVA programming language Level 2 : 4 - 6.7.01 (3 days) Enterprise JavaBeans :  9 - 11.7.01 (3 days) Design Patterns :  10 - 12.7.01 (3 days) C++ for Particle Physicists :  23 - 27.7.01 (6 3-hour lectures) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  13. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction to Perl 5 : 2 - 3.7.01 (2 days) Introduction to Databases :  3 - 4.7.01 (2 days) JAVA programming language Level 2 : 4 - 6.7.01 (3 days) Enterprise JavaBeans :  9 - 11.7.01 (3 days) Design Patterns :  10 - 12.7.01 (3 days) C++ for Particle Physicists :  23 - 27.7.01 (6 3-hour lectures) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  14. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) Introduction to PVSS : 16.6.03 (p.m.) Basic PVSS : 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) PVSS - JCOP Framework Tutorial : 20.6.03 (1 day) Programmation automate Schneider : Programmation automate Schneider TSX Premium - 2ème niveau : 24 - 27.6.03 (4 jours) - audience : toute personne qui veux maitriser la mise en uvre et la programmation des fonctions spécialisées d'un automate TSX Premium - objectifs : maitriser la mise en uvre et la programmation des fonctions spécialisées d'un automate TSX Premium Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (session de 3 jours) ** The number of places available may vary. Please check our Web site to find out the current availability. If you wish to participate in one of these courses, pl...

  15. Evaluation of user satisfaction of hearing aids (HA in the Amazon

    Directory of Open Access Journals (Sweden)

    Arakawa, Aline Megumi

    2010-03-01

    Full Text Available Introduction: The main function of human hearing is enabling oral communication. In this sense, hearing loss impairs severely communication skills and social relationships of individuals. Therefore, the project "USP in Rondônia" of FOB/USP conducts expeditions travelling to the municipality from Monte Negro/RO allowing the promotion of hearing health. Objective: To assess the level of satisfaction user with hearing aids (HA. Method: Was accomplished a prospective study of 18 individuals with hearing loss fitted with hearing aids in the Clinic of Oral and Fono Audiological Health from Monte Negro/RO. For the evaluation, we used the questionnaire for self-assessment IOI-HA (International Outcome Inventory for Hearing Aids. Results: Concerning the seven domains assessed, it was verified that the average referring to the use was 4.2, the benefit was 3.9, the limiting of residual activity was 3.7; the satisfaction was 4.4, the restriction of participation of residual activity was 3.8, the impact on others was 4.3 and 3.9 for the quality of life. Respecting the factors one and two, it was applied the statistical test t-Student founding no statistically significant difference. However, the analysis of the score relative to factors one and two showed good results as the individual's interaction with his hearing aid and with their environment, respectively. Conclusion: With this study, we can demonstrate the high grade of satisfaction from the use of hearing aids presented by the majority of the sample collected in all domains analyzed.

  16. Androgen-stimulated UDP-glucose dehydrogenase expression limits prostate androgen availability without impacting hyaluronan levels

    Science.gov (United States)

    Wei, Qin; Galbenus, Robert; Raza, Ashraf; Cerny, Ronald L.; Simpson, Melanie A.

    2009-01-01

    UDP-glucose dehydrogenase (UGDH) oxidizes UDP-glucose to UDP-glucuronate, an essential precursor for production of hyaluronan (HA), proteoglycans, and xenobiotic glucuronides. High levels of HA turnover in prostate cancer are correlated with aggressive progression. UGDH expression is high in the normal prostate even though HA accumulation is virtually undetectable. Thus, its normal role in the prostate may be to provide precursors for glucuronosyltransferase enzymes, which inactivate and solubilize androgens by glucuronidation. In this report, we quantified androgen dependence of UGDH, glucuronosyltransferase, and HA synthase expression. Androgen dependent and independent human prostate cancer cell lines were used to test the effects of UGDH manipulation on tumor cell growth, HA production and androgen glucuronidation. Dihydrotestosterone (DHT) increased UGDH expression ≈2.5-fold in androgen dependent cells. However, upregulation of UGDH did not affect HA synthase expression or enhance HA production. Mass spectrometric analysis showed that DHT was converted to a glucuronide, DHT-G, at a six-fold higher level in androgen dependent cells relative to androgen independent cells. The increased solubilization and elimination of DHT corresponded to slower cellular growth kinetics, which could be reversed in androgen dependent cells by treatment with a UDP-glucuronate scavenger. Collectively, these results suggest that dysregulated expression of UGDH could promote the development of androgen independent tumor cell growth by increasing available levels of intracellular androgen. PMID:19244115

  17. 76 FR 18298 - Notice of Availability of a Final Environmental Impact Statement for the DesertXpress High-Speed...

    Science.gov (United States)

    2011-04-01

    ... Considering Environmental Impacts (64 FR 28545, May 26, 1999). The Bureau of Land Management (BLM), Surface... DesertXpress High-Speed Passenger Train Project AGENCY: Federal Railroad Administration (FRA), United... High-Speed Passenger Train Project (DesertXpress project). FRA is the Lead Agency for the...

  18. PLACES AVAILABLE

    CERN Multimedia

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    2002-01-01

    Places are available in the following courses: The CERN Engineering Data Management System for Advanced users :  13.6.02  (1 day) The CERN Engineering Data Management System for Local Administrators :  18.6.02  (1 day) AutoCAD 2002 - niveau 2 : 24 - 25.6.02 (2 jours) Frontpage 2000 - niveau 2 : 25 - 26.6.02 (2 jours) Object-oriented Analysis and Design :  2 - 5.7.02  (4 days) C++ Programming Level 2 - Traps & Pitfalls :  16 - 19.7.02  (4 days) C++ for Particle Physicists :  22 - 26.7.02  (6 * 3 hour lectures) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of the...

  19. PLACES AVAILABLE

    CERN Multimedia

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    2002-01-01

    Places are available in the following courses: LabView Hands-on (bilingue/bilingual - gratuit/free of charge) : 13.9.02 (a.m.) LabView DAQ Hands-on (bilingue/bilingual - gratuit/free of charge) : 13.9.02 (p.m.) AutoCAD 2002 - niveau 1 : 19, 20, 26, 27.9.02 (4 jours) LabView Base 1 : 23 - 25.9.02 (3 jours) LabView DAQ (E) : 26 - 27.9.02 (2 days) AutoCAD Mechanical 6 PowerPack (F) : 30.9, 1, 2, 9, 10, 11.10.02 (6 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 10.10.02 (half-day, p.m.) AutoCAD 2002 - niveau 2 : 14 - 15.10.02 (2 jours) AutoCAD 2002 - Level 1 : 17, 18, 24, 25.10.02 (4 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Of...

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    2001-01-01

    Places are available in the following courses: Utilisation du simulateur Simplorer : 30.5 - 1.6.01 (3 jours) JAVA programming language level 1: 11-12.6.01 (2 days) LabView hands-on F ou E : 11.6.01 (1/2 journée) Comprehensive VHDL for EPLD/FPGA Design : 11 - 15.6.01 (5 days) Introduction au Langage C : 13 - 15.6.01 (3 jours) LabView Base 1 : 12 - 14.6.01 (3 jours) Habilitation électrique : superviseurs : 2 sessions d'une demi-journée les 12 et 19.6.01 Migration de LabVIEW 5 vers LabVIEW 6i Migration from LabVIEW 5 to LabVIEW 6I :  15.6.01 (1/2 journée/half-day) Introduction to Perl 5 : 2 - 3.7.01 (2 days) JAVA programming language level 2 : 4 - 6.7.01 (3 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from ...

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    Technical Training; Tel. 74924

    2000-01-01

    Places are available in the following courses:   C++ for Particle Physicists 20 - 24.11.00 6 lectures CANbus 20.11.00 1 journée CANopen 21 et 22.11.00 2 jours Sécutiré dans les installations cryogéniques 21 et 22.11.00 2 demi-journées The JAVA programming language level 2 27 ­ 29.11.00 3 days Contract Follow-up 27.11.00 3 heures 1/2 Cryogénie (introduction) 4 ­ 8.12.00 ANSYS Introduction : langue a décider suivant majorité 5 ­ 7.12.00 3 jours EXCEL 7, 8, 13 et 14.12.00 4 jours Contract Follow-up 15.12.00 3 heures 1/2 If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an “application for training” form available from your Divisional Secretariat or from your DTO (Divisional Training Officer)....

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    2002-01-01

    Places are available in the following courses: Habilitation électrique : recyclage HT/BT : 11 - 15.3.2002  (2 * 2 heures) PVSS Basics :  8 - 12.4.02  (5 days) ELEC-2002 : Spring Term :  9, 11, 16, 18, 23, 25, 30.4.02 (7 * 2.5 hours) LabVIEW base 1 : 22 - 24.4.02 (3 jours) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) LabVIEW Basics 2 : 13 & 14.5.02 (2 days) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applica...

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    Technical Training; Tel 74924

    2002-01-01

    Places are available in the following courses: LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 24 & 25.01.02 (2 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ (E) : 7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java : 11 - 13.02.02 (3 days) C++ for Particle Physicists : 11 - 15.3.2002 (6 * 3 hour lectures) Cours sur la migration AutoCAD : AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO ...

  4. PLACES AVAILABLES

    CERN Multimedia

    Enseignement Technique; Tél. 74924; monique.duval@cern.ch

    2000-01-01

    Places are available in the following courses: LabView hands-on : 13.11.00 (4 hours) LabView Basics 1 : 14 ­ 16.11.00 (3 days) Nouveautés de WORD : 19 et 20.10.00 (2 jours) ACCESS 1er niveau : 30 ­ 31.10.00 (2 jours) Advanced C programming : 2 ­ 3.11.00 (2 days) Introduction à PowerPoint : 6.11.00 (1 journée) C++ for Particle Physicists : 20 ­ 24.11.00 (12 hours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an “application for training” form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  5. PLACES AVAILABLE

    CERN Multimedia

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    2002-01-01

    Places are available in the following courses: PVSS basics :  18 - 22.2.02 (5 days) Introduction à la CAO CADENCE : 20 & 21.2.02 (2 jours) LabView Basics 1 :  4 - 6.3.02  (3 days) Introduction au VHDL et utilisation du simulateur de CADENCE : 6 & 7.3.02 (2 jours) LabView Base 2 : 11 & 12.3.02 (2 jours) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) LabView Advanced :  13 - 15.3.02 (3 days) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO...

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    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction à la CAO CADENCE : 20 & 21.2.02 (2 jours) LabView Basics 1 :  4 - 6.3.02  (3 days) Introduction au VHDL et utilisation du simulateur de CADENCE : 6 & 7.3.02 (2 jours) CLEAN-2002 : Working in a Clean Room :  7.3.2002  (1 day) LabView Base 2 : 11 & 12.3.02 (2 jours) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) LabView Advanced :  13 - 15.3.02 (3 days) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisiona...

  7. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: Hands-on Introduction to Python Programming: 11-13.08.2003(3 days) Introduction to the CERN Engineering Data Management System (EDMS): 26.08.2003 (1 day) The CERN Engineering Data Management System (EDMS) for Engineers: 27.08.2003 (1 day) CLEAN-2002 : Travailler en salle blanche : 4.09.2003 (une demi-journée) AutoCAD 2002 - Level 1: 4, 5, 15, 16.09.2003 (2 x 2 days) AutoCAD Mechanical 6 PowerPack : 17, 18, 25, 26.09.2003 et 2, 3.10.2003 (3 x 2 journées, français) AutoCAD 2002 - niveau 1 : 23, 24, 30.09.2003 et 1.10.2003 (2 x 2 journées) Introduction to the CERN Engineering Data Management System (EDMS): 23.09.2003 (1 day) The CERN Engineering Data Management System (EDMS) for Local Administrators: 24-25.09.2003 (2 days) AutoCAD 2002 - niveau 2 : 8 et 10.10.2003 (2 journées) CLEAN-2002: Working in a Cleanroom: 23.10.2003 (half day, p.m.) ** The number of places available may vary. Please ch...

  8. Places available**

    CERN Multimedia

    Places are available in the following courses: Hands-on Introduction to Python Programming: 11-13.08.2003 (3 days) Introduction to the CERN Engineering Data Management System (EDMS): 26.08.2003 (1 day) The CERN Engineering Data Management System (EDMS) for Engineers: 27.08.2003 (1 day) CLEAN-2002 : Travailler en salle blanche : 4.09.2003 (une demi-journée) AutoCAD 2002 - Level 1: 4, 5, 15, 16.09.2003 (2 x 2 days) AutoCAD Mechanical 6 PowerPack : 17, 18, 25, 26.09.2003 et 2, 3.10.2003 (3 x 2 journées, français) AutoCAD 2002 - niveau 1 : 23, 24, 30.09.2003 et 1.10.2003 (2 x 2 journées) Introduction to the CERN Engineering Data Management System (EDMS): 23.09.2003 (1 day) The CERN Engineering Data Management System (EDMS) for Local Administrators: 24-25.09.2003 (2 days) AutoCAD 2002 - niveau 2 : 8 et 10.10.2003 (2 journées) CLEAN-2002: Working in a Cleanroom: 23.10.2003 (half day, p.m.) ** The number of places available may vary. Please check our Web site to find out the current availabili...

  9. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Java Programming Language level 1 :  28 & 29.11.02  (2 days) December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training M...

  10. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Hands-on Object-Oriented Design and Programming with C++:  19 - 21.11.02  (3 days)  December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) AutoCAD 2002 - niveau 2 :  2 & 3.12.02  (2 jours) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02  (2 jours) PCAD PCB - Débutants :  9 - 11.12.02  (3 jours) FrontPage 2000 - level 1:  9 & 10.12.02  (2 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : Technical Training or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisiona...

  11. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74460

    2001-01-01

    Places are available in the following courses: LabView Base 1 : 27-29.3.01 (3 jours) Contract Follow-up : 9.4.01 (3 heures) Introduction à PowerPoint : 24.4.01 (1 journée) Publier sur le Web : 25-27.4.01 (3 demi-journées) Programmation TSX Premium 2 : 15-16.5.01 (5 jours) LabView Base 2 : 27-29.3.01 (2 jours) Hands-on Object-oriented Analysis, Design & Programming with C++ :  23-27.4.01 (5 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at : http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  12. Endothelial cell cultured on HA/TCP/chitosan scaffold for bone tissue engineering

    Directory of Open Access Journals (Sweden)

    Bachtiar EW

    2011-06-01

    Full Text Available Background: Angiogenesis is crucial for the success of bone reconstruction through tissue engineering. Currently, is still not known the activity of endothelial cells that is responsible for blood vessel formation, cultured in HA/TCP/chitosan scaffold. The ability of the scaffold to facilitate the proliferation and migration of endothelial cell to form blood vessel is essential for cell survival especially in the inner area of the scaffold that is susceptible for cell death if adequate vascularization is not occurred. Purpose: The purpose of this study was to evaluate the porosity of HA/TCP/chitosan scaffold and the biocompatibility of HA/TCP/chitosan scaffold to endothelial cells. Methods: Endothelial cells were isolated from umbilical vein (human umbilical vein endothelial cells/ HUVEC. HA/ TCP/chitosan scaffold was made from two gelling agents and various basic washing solutions. The characteristic of scaffold was examined by scanning electron microscopy. The activity of HUVEC was evaluated by MTT assay. Results: Initial average scaffold porosity size range from 68 μm and increased up to 134 μm after 7 days incubation with 10 mg/L lysozyme. There was no significant difference in the viability of HUVEC incubated with the scaffold compared to control. Conclusion: HA/TCP/chitosan has a good biocompatibility for HUVEC. This condition supports the activity of HUVEC in the scaffold for angiogenesis process, to provide oxygen and nutrient necessary for osteoblast.Latar belakang: Angiogenesis merupakan proses yang penting untuk keberhasilan rekonstruksi tulang melalui rekayasa jaringan. Saat ini, aktifitas sel endotel pembentuk dinding pembuluh darah pada HA/TCP/chitosan scaffold belum diketahui. Kemampuan scaffold sebagai tempat proliferasi dan migrasi sel endotel untuk membentuk pembuluh darah penting untuk kelangsungan hidup sel osteoblast terutama di bagian dalam scaffold. Tujuan: Mengevaluasi porositas HA/TCP/chitosan scaffold serta sifat

  13. The Effects of Different Tillage Methods on Available Soil Potassium Measured by Various Extractors in a Soil with High Specific Surface Area

    Directory of Open Access Journals (Sweden)

    M. Hosseini

    2016-02-01

    Full Text Available Introduction: The effects of any tillage method on soil properties, depends on location (soil, water and air and the number of (years their implementation. Soil compaction reduces yield through increased soil mechanical resistance against root growth and lower water and nutrient use efficiency (Gamda et al. 18 & Ishagh et al 23. Soil surface and sub surface compaction both reduce yield due to limited root growth and plant potassium uptake (Doulan et al. 14. Sabt et al. (50 reported that in the study area, which the lands are mostly illite clay (high specific surface area with sufficient nitrogen, soil potassium is the most important limiting factor for the growth of wheat.Considering the point that loess soils in Golestan Province have a high specific surface area,they can provide potassium for plants to produce crop, but for a higher production, potassium fertilizers should be used. Previous studies indicated that production of wheat is limited due to potassium deficiency (4, 49, 54 and 57. In these soils with a high specific surface area, the speed of movement of potassium from the soil solution is low, and doing solimits wheat yield.In loess soils containing high illite and high specific surface area (eg, soilsin the series of Rahmat Abad of Gorgan, ammonium acetate measured potassium on exchange and solution surfaces, which is highly correlated with grain yield (54 . There is a high correlation between grain yield with overload of potassium and Na TPB extraction (57. The aim of this study was to absorb potassium (limiting factor for plant growth with different tillage systemsat different depths. International recommendations towards reducing the depth and intensity of tillage (from minimum tillage to no-tillage in order to reduce erosion and oxidation of organic substances plays an important role in determining the amount of greenhouse gases. If potassium absorption does not reduceafter reducing tillage intensity,low or no-tillage methods

  14. [Not Available].

    Science.gov (United States)

    Meyer, J J; Paumard, J L; Milin, D; Levoir, P; Fontaine, J C

    1988-11-01

    A low-cost digital acquisition unit for a CARY-210 spectrophotometer has been designed and constructed. The data-flow is controlled by a 6809 microprocessor driving four parallel interface adapters. Two RS232-C serial links are provided for communication with a terminal and a mass storage unit. The acquired data are processed by a personal computer. Included are several software modules, based on powerful signal-processing algorithms, which are highly useful in kinetic studies.

  15. Possible Detection of the Stellar Donor or Remnant for the Type Iax Supernova 2008ha

    CERN Document Server

    Foley, Ryan J; Jha, Saurabh W; Bildsten, Lars; Fong, Wen-fai; Narayan, Gautham; Rest, Armin; Stritzinger, Maximilian D

    2014-01-01

    Type Iax supernovae (SNe Iax) are thermonuclear explosions that are related to SNe Ia, but are physically distinct. The most important differences are that SNe Iax have significantly lower luminosity (1% - 50% that of typical SNe Ia), lower ejecta mass (~0.1 - 0.5 M_sun), and may leave a bound remnant. The most extreme SN Iax is SN 2008ha, which peaked at M_V = -14.2 mag, about 5 mag below that of typical SNe Ia. Here, we present Hubble Space Telescope (HST) images of UGC 12682, the host galaxy of SN 2008ha, taken 4.1 years after the peak brightness of SN 2008ha. In these deep, high-resolution images, we detect a source coincident (0.86 HST pixels; 0.043"; 1.1 sigma) with the position of SN 2008ha with M_F814W = -5.4 mag. We determine that this source is unlikely to be a chance coincidence, but that scenario cannot be completely ruled out. If this source is directly related to SN 2008ha, it is either the luminous bound remnant of the progenitor white dwarf or its companion star. The source is consistent with ...

  16. CILogon-HA. Higher Assurance Federated Identities for DOE Science

    Energy Technology Data Exchange (ETDEWEB)

    Basney, James [Univ. of Illinois, Urbana-Champaign, IL (United States)

    2015-08-01

    The CILogon-HA project extended the existing open source CILogon service (initially developed with funding from the National Science Foundation) to provide credentials at multiple levels of assurance to users of DOE facilities for collaborative science. CILogon translates mechanism and policy across higher education and grid trust federations, bridging from the InCommon identity federation (which federates university and DOE lab identities) to the Interoperable Global Trust Federation (which defines standards across the Worldwide LHC Computing Grid, the Open Science Grid, and other cyberinfrastructure). The CILogon-HA project expanded the CILogon service to support over 160 identity providers (including 6 DOE facilities) and 3 internationally accredited certification authorities. To provide continuity of operations upon the end of the CILogon-HA project period, project staff transitioned the CILogon service to operation by XSEDE.

  17. 75 FR 17368 - Notice of Availability of an Evaluation of the Highly Pathogenic Avian Influenza Status of Czech...

    Science.gov (United States)

    2010-04-06

    ... the Czech Republic and Sweden with respect to HPAI H5N1. We are making this evaluation available to... Board of Agriculture reported their first case of HPAI H5N1 in domestic poultry to the World Organization of Animal Health (OIE). The outbreak was confirmed in mallard ducks on a game bird breeding farm...

  18. Drought response of five conifer species under contrasting water availability suggests high vulnerability of Norway spruce and European larch

    NARCIS (Netherlands)

    Lévesque, M.; Saurer, M.; Siegwolf, R.; Eilmann, B.; Brang, P.; Bugmann, H.; Rigling, A.

    2013-01-01

    The ability of tree species to cope with anticipated decrease in water availability is still poorly understood. We evaluated the potential of Norway spruce, Scots pine, European larch, black pine, and Douglas-fir to withstand drought in a drier future climate by analyzing their past growth and

  19. Sediment availability on burnt hillslopes

    Science.gov (United States)

    Nyman, Petter; Sheridan, Gary; Moody, John; Smith, Hugh; Noske, Philip; Lane, Patrick

    2013-04-01

    In general, erosion has been modeled as being proportional to some form of energy or force (such as shear stress or stream power) with the proportionality constant being erodibility, which is a characterization of sediment availability. It is unclear if erodibility is constant with depth on recently burnt hillslopes. This study used both field- and laboratory based experiments to quantify sediment availability as a depth-dependent parameter on burnt hillslopes. An explicit representation of fire-effect on sediment availability was achieved by assuming that fire-effects produce a non-cohesive soil layer of variable depth. This depth is characterized as a probability density function with a single parameter that changed during recovery (0-3 years) as the available soil was depleted. Measurements in southeastern Australia found that initially after a wildfire the hillslope had a layer (0.75-0.91 cm in depth) of non-cohesive soil, which represented 97-117 t/ha of transport limited sediment. The thickness of this layer decreased exponentially with time since the wildfire. Additional results showed that fine roots (soils for depths soil depth and root density accounted for ~60 % of variation in the erodibility at soil depths soil properties (% silt and clay in particular) became more important as predictors of erodibility. The results are organized into a conceptual framework for modeling fire-effects on sediment availability for systems with low and high pre-fire erodibility. The fire-effect produces an equal depth of non-cohesive soil for both systems but this represents a greater perturbation for systems with low pre-fire erodibility than for those systems with a high pre-fire erodibility.

  20. Functional study of the novel multidrug resistance gene HA117 and its comparison to multidrug resistance gene 1

    Directory of Open Access Journals (Sweden)

    Chen Tingfu

    2010-07-01

    Full Text Available Abstract Background The novel gene HA117 is a multidrug resistance (MDR gene expressed by all-trans retinoic acid-resistant HL-60 cells. In the present study, we compared the multidrug resistance of the HA117 with that of the classical multidrug resistance gene 1 (MDR1 in breast cancer cell line 4T1. Methods Transduction of the breast cancer cell line 4T1 with adenoviral vectors encoding the HA117 gene and the green fluorescence protein gene (GFP (Ad-GFP-HA117, the MDR1 and GFP (Ad-GFP-MDR1 or GFP (Ad-GFP was respectively carried out. The transduction efficiency and the multiplicity of infection (MOI were detected by fluorescence microscope and flow cytometry. The transcription of HA117 gene and MDR1 gene were detected by reverse transcription polymerase chain reaction (RT-PCR. Western blotting analysis was used to detect the expression of P-glycoprotein (P-gp but the expression of HA117 could not be analyzed as it is a novel gene and its antibody has not yet been synthesized. The drug-excretion activity of HA117 and MDR1 were determined by daunorubicin (DNR efflux assay. The drug sensitivities of 4T1/HA117 and 4T1/MDR1 to chemotherapeutic agents were detected by Methyl-Thiazolyl-Tetrazolium (MTT assay. Results The transducted efficiency of Ad-GFP-HA117 and Ad-GFP-MDR1 were 75%-80% when MOI was equal to 50. The transduction of Ad-GFP-HA117 and Ad-GFP-MDR1 could increase the expression of HA117 and MDR1. The drug resistance index to Adriamycin (ADM, vincristine (VCR, paclitaxel (Taxol and bleomycin (BLM increased to19.8050, 9.0663, 9.7245, 3.5650 respectively for 4T1/HA117 and 24.2236, 11.0480, 11.3741, 0.9630 respectively for 4T1/MDR1 as compared to the control cells. There were no significant differences in drug sensitivity between 4T1/HA117 and 4T1/MDR1 for the P-gp substrates (ADM, VCR and Taxol (P Conclusions These results confirm that HA117 is a strong MDR gene in both HL-60 and 4T1 cells. Furthermore, our results indicate that the MDR

  1. [Not Available].

    Science.gov (United States)

    Blanchard, Elodie; de Lara, Manuel Tunon

    2013-01-01

    Pholcodine is an opioid that has been widely used worldwide since 1950 for the treatment of non-productive cough in children and adults. The results of early preclinical studies but also those of recent clinical trials have shown the antitussive efficacy of pholcodine to be superior to that of codeine, of longer duration, and with an equivalent or safer toxicity profile. Also, there is no risk of addiction. Concern had been raised over a possible cross-sensitisation with neuromuscular blocking agents. While a recent assessment of the available data by the European Medicines Agency (EMA) has confirmed the favourable risk-benefit ratio of pholcodine, further studies are needed to clear this point.

  2. [Not Available].

    Science.gov (United States)

    Murray, Clinton K; Bennett, Jason W

    2009-01-01

    Malaria's global impact is expansive and includes the extremes of the healthcare system ranging from international travelers returning to nonendemic regions with tertiary referral medical care to residents in hyperendemic regions without access to medical care. Implementation of prompt and accurate diagnosis is needed to curb the expanding global impact of malaria associated with ever-increasing antimalarial drug resistance. Traditionally, malaria is diagnosed using clinical criteria and/or light microscopy even though both strategies are clearly inadequate in many healthcare settings. Hand held immunochromatographic rapid diagnostic tests (RDTs) have been recognized as an ideal alternative method for diagnosing malaria. Numerous malaria RDTs have been developed and are widely available; however, an assortment of issues related to these products have become apparent. This review provides a summary of RDT including effectiveness and strategies to select the ideal RDT in varying healthcare settings.

  3. Mechanical, thermal, rheological and morphological behaviour of irradiated PP/HA composites

    Energy Technology Data Exchange (ETDEWEB)

    Ramirez, C. [Universidad de Oriente, Nucleo de Anzoategui, Escuela de Ingenieria y Ciencias Aplicadas, Depto de Ingenieria Quimica, Puerto la Cruz (Venezuela); Albano, C. [Centro de Quimica, Laboratorio de Polimeros, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas 1020A (Venezuela) and Universidad Central de Venezuela, Facultad de Ingenieria, Escuela de Ingenieria Quimica, Caracas 1041A (Venezuela)]. E-mail: calbano@ivic.ve; Karam, A. [Centro de Quimica, Laboratorio de Polimeros, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas 1020A (Venezuela)]. E-mail: akaram@quimica.ivic.ve; Dominguez, N. [Centro de Quimica, Laboratorio de Polimeros, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas 1020A (Venezuela); Sanchez, Y. [Centro de Quimica, Laboratorio de Polimeros, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas 1020A (Venezuela); Gonzalez, G. [Centro Tecnologico, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas 1020A (Venezuela)

    2005-07-01

    Hydroxyapatite (HA) reinforced polypropylene (PP) composites are being developed as bone graft materials. In this research, the effect of {gamma} irradiation on mechanical, rheological, thermal and morphological behaviour of PP-HA composites was studied. The melt flow index of polymer increased markedly when it was exposed to radiation. This is indicative of chain scission reaction as the predominant process. During the tensile testing, the composites exhibited brittle behaviour, showing no fluency point. Elongation at break showed a tendency to decrease with the increase in radiation dose while stress at break did not show significant variation with radiation dose. High HA content (>20%) and radiation dose (25 kGy) had significant influence on thermal stability.

  4. Difference of adherence, proliferation and osteogenesis of mesenchymal stem cells cultured on different HA/ZrO2 composites

    Institute of Scientific and Technical Information of China (English)

    QUAN Ren-fu; TANG Yang-hua; HUANG Zhong-ming; YANG Di-sheng; LI Wei; XU Jin-wei; WU Xiao-chun

    2012-01-01

    Objective: To study the adherence,proliferation and osteogenesis of mesenchymal stem cells (MSCs) cultured on different HA/ZrO2 composites.Methods: The simplex and graded HA/ZrO2 composites were prepared using dry-laid method.The surface topography of the composites was observed by scanning electron microscope (SEM).The MSCs were isolated from rabbits and cultured on experimental groups (simplex HA/ ZrO2 composite,graded HA/ZrO2 composite,pure HA or pure ZrO2 coatings respectively) and control group (ordinary culture plate).Then,we observed the adherence,proliferation and osteogenesis of the MSCs,detected the cellular alkaline phosphatase (ALP) activities,extracted total RNA and detected the mRNA expression of collagen Ⅰ,osteocalcin and osteopontin using the reverse transcription and polymerase chain reaction (RT-PCR) method.Results: The SEM images confirmed that the surfaceof the simplex HA/ZrO2 composite was coverd by discontiguous HA layer with clear visualization of the partial ZrO2 matrix,while the surface of the graded HA/ZrO2 composite was fairly rough with porosity.X-ray diffraction showed that after high temperature sintering,the ZrO2 phase still remained,while the HA phase was transformed to β-Ca3 (pO4)2,α-Ca3(PO4)2 and CaZrO3 phases on the surface of both composites.Cell culture indicated that the HA/ ZrO2 composites supported cell attachment.Neither ALP expression nor mRNA expression of collagen Ⅰ,osteocalcin or osteopontin from RT-PCR results showed significant deviation among four groups.Conclusion:Among these four composites,the graded HA/ZrO2 composite promotes the MSCs proliferation and the osteogenic differentiation to a certain extent.

  5. High energy SEU test results for the commercially available MIPS R3000 microprocessor and R3010 floating point unit

    Energy Technology Data Exchange (ETDEWEB)

    Shaeffer, D.L.; Kimbrough, J.R.; Denton, S.M.; Kaschimitter, J.L.; Wilburn, J.W.; Davis, R.W.; Colella, N.J. (Lawrence Livermore National Lab., CA (United States)); Holtkamp, D.B. (Los Alamos National Lab., NM (United States))

    1991-08-15

    Single event upset (SEU) cross sections and total dose hardness of commercially available MIPS R3000 microprocessors (CPU) and R3010 floating point units (FPU) were obtained by exposing these parts to 256 MeV protons from the linear accelerator (LINAC) at the Los Alamos Meson Physics Facility (LAMPF). Parts from several manufacturers were tested. The CPUs and FPUs were tested dynamically during radiation exposure with specially designed assembly language codes which exercised a subset of the available instructions in order to simulate the actual operation of each part. Cross sections derived from the SEU data were used to calculate expected upset rate for a 500-km orbit during quiet solar conditions, the King 1972 solar flare, and the August 4, 1972, event modeled by Adams et al. 16 refs., 16 figs., 3 tabs.

  6. High energy proton SEU test results for the commercially available MIPS R3000 microprocessor and R3010 floating point unit

    Energy Technology Data Exchange (ETDEWEB)

    Shaeffer, D.L.; Kimbrough, J.R.; Denton, S.M.; Kaschmiter, J.L.; Wilburn, J.W.; Davis, R.W.; Colella, N.J. (Lawrence Livermore National Lab., CA (United States)); Holtkamp, D.B. (Los Alamos National Lab., NM (United States))

    1991-12-01

    This paper reports on proton single event upset (SEU) cross sections and proton total dose hardness of commercially available MIPS R3000 microprocessors (CPU) and R3010 floating point units (FPU) that were obtained by exposing these parts to 256 MeV protons from the linear accelerator (LINAC) at the Los Alamos Meson Physics Facility (LAMPF). Parts from several manufacturers were tested. The CPUs and FPUs were tested dynamically during radiation exposure with specially designed assembly language codes which exercised a subset of the available instructions. Cross sections derived from the SEU data were used to calculate expected upset rates for a 500-km, 60-degree inclination, orbit during quiet solar conditions and during the August 4, 1972, King solar flare event.

  7. Optimising power plant operation - high availability at low maintenance costs; Optimierung des Kraftwerksbetriebs - hohe Verfuegbarkeit trotz geringer Instandhaltungskosten

    Energy Technology Data Exchange (ETDEWEB)

    Kienreich, A.; Maagh, P.; Gilow, C.; Suerig, C.; Vahlenkamp, T. [McKinsey and Co., Inc., Duesseldorf (Germany)

    2008-01-15

    Most power plant operators responded to the liberalisation of the European energy markets by lowering their costs. The measures they took to this end such as lengthening maintenance intervals, cutting down on operating staff and progressively outsourcing maintenance services have indeed had an impact, albeit not as intended. In the meantime some power plants are showing first signs of impaired availability and reliability, both of which criteria had begun to play a more prominent role again in recent years. Today's challenge on power supply companies therefore is to optimise the costs and availability of their plants simultaneously. The ''McKinsey Power Gauge'', a Europe-wide benchmarking system provides assistance in identifying successful operating and maintenance strategies.

  8. A complete analysis of HA and NA genes of influenza A viruses.

    LENUS (Irish Health Repository)

    Shi, Weifeng

    2010-12-01

    More and more nucleotide sequences of type A influenza virus are available in public databases. Although these sequences have been the focus of many molecular epidemiological and phylogenetic analyses, most studies only deal with a few representative sequences. In this paper, we present a complete analysis of all Haemagglutinin (HA) and Neuraminidase (NA) gene sequences available to allow large scale analyses of the evolution and epidemiology of type A influenza.

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    Places are available in the following courses : DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) LabView DAQ (language to be defined) : 8 & 9.5.03 AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) AutoCAD 2002 - niveau 2 : 3 & 4.6.03 (2 jours) LabView DSC (language to be defined) : 19 & 20.6.03 Siemens SIMATIC Training : Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 16 - 20.6.03 (5 days) Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 (sessions of 2 days) These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (session de 3 jours) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description ...

  3. [Not Available].

    Science.gov (United States)

    Calbiac, Pascale De; Lamoureux, Fabien; Pourrat, Xavier; Bretault, Lydia; Marchand, Sophie; Grassin, Jacqueline; Antier, Daniel

    2006-01-01

    Treatment of Bronchial Superinfections: Data Related to Stability of Antibiotics in Portable Pumps. Given many data about the stability of antibiotics in portable pump (elastomer) are lacking, this study was designed to make a point about available data and to evaluate the stability of antibiotics when exposed to temperature within 35°C (average temperature measured in real conditions of use). First, to collect information about the stability of antibiotics in portable pump and to confront them with the local antibiotics protocols dedicated to the treatment of bronchial superinfection in patients with cystic fibrosis; second, to evaluate the stability of piperacillin associated with tazobactam at 35°C. While measured concentrations in tazobactam did not show significant variation during the study, piperacillin measurements showed a major reduction of concentration (up to 33%), both time and concentration related to. Such information must be pointed out to prescribers and patients to ensure a cold accumulator is placed in the pump can'ying-bag and to limit the duration of infusion to 24h with a single pump. This experimental program will keep on going with the stability study of both ticarcillin and cefsulodin in portable pump. Copyright © 2006 Société Française de Pharmacologie et de Thérapeutique. Publié par Elsevier Masson SAS.

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    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: LabVIEW - Basics 1 :  10 - 12.12.01 (3 days) Introduction to XML :  12 & 13.12.01 (2 days) Introduction au PC et Windows 2000 : 12 & 14.12.01 (2 jours) LabVIEW - Basics 2 :  13 - 14.12.01 (2 days) Habilitation électrique : superviseurs : 17.12.2001 (1/2 journée) MS-Project 2000 : 10 & 11.01.02 (2 jours) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) Sécurité dans les installations cryogéniques: 15-17.1.2002 (2 demi-journées) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) ELEC-2002 Winter Term: Readout and system electronics for Physics  15.1.2002 - 7.2.2002 (8 half- days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 -...

  7. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2002-01-01

    Places are available in the following courses: LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 22, 24 & 25.01.02 (3 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ  (E) :  7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java :  11 - 13.02.02 (3 days) PVSS basics :  11 - 15.2.02 (5 days) Introduction à Windows 2000 : 18.2.02 (1 demi-journée) Introduction to the CERN Engineering Data Management System :  20.2.02 (1 day) The CERN Engineering Data Management System for Advanced users :  21.2.02  (1 day) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) Cours sur la migration AutoCAD : AutoCAD : Mise à...

  8. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel.74924

    2001-01-01

    Places are available in the following courses: Habilitation électrique : superviseurs : 5.12.01 (1/2 journée) LabVIEW - Basics 1 :  10 - 12.12.01 (3 days) Introduction au PC et Windows 2000 : 12 & 14.12.01 (2 jours) LabVIEW - Basics 2 :  13 - 14.12.01 (2 days) Habilitation électrique : superviseurs : 17.12.2001 (1/2 journée) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) Sécurité dans les installations cryogéniques: 15-17.1.2002 (2 demi-journées) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) ELEC-2002 Winter Term: Readout and system electronics for Physics  15.1.2002 - 7.2.2002 (8 half- days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) Frontpage...

  9. PLACES AVAILABLE

    CERN Multimedia

    Enseignement Technique; Tel. 74924

    2001-01-01

    Places are available in the following courses: MS-Project 2000 : 10 & 11.01.02 (2 jours) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) Sécurité dans les installations cryogéniques: 15-17.1.2002 (2 demi-journées) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) ELEC-2002 Winter Term: Readout and system electronics for Physics  15.1.2002 - 7.2.2002 (8 half- days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) MS-Project 2000 : 24 & 25.01.02 (2 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1.02 (2 jours) LabView Base 1 : 4 - 6.2.02 (3 jours) LabView DAQ  (E) :  7 & 8.02.02 (2 days) Hands-on Object-Oriented Design & Programming with Java :&nbs...

  10. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: PVSS Basics :  8 - 12.4.02  (5 days) AutoCAD : Mise à jour AutoCAD r-14 vers 2002 : 25 & 26.4.02 (2 jours) ELEC-2002 : Spring Term :  9, 11, 16, 18, 23, 25, 30.4.02 (7 * 2.5 hours) Object-Oriented Analysis & Design: 16 - 19.4.02  (4 days) Migration from AutoCAD 14 towards AutoCAD Mechanical6 PowerPack:  17 - 19.4 and 2 &3.5.02  (5 days) LabVIEW base 1 : 22 - 24.4.02 (3 jours) LabVIEW DSC (F) 25 & 26.4.02 (2 jours) LabVIEW Basics 2 : 13 & 14.5.02 (2 days) EXCEL 2000 - niveau 2 : 22 & 23.5.02 (2 jours) LabVIEW DAQ (F) : 15 & 16.5.02 (2 jours) LabVIEW Basics 1:  3 - 5.6.02  (3 days) LabVIEW DAQ (E):  6 & 7.6.02  (2 days) If you wish to participate in one of these courses, please discuss with your supervisor and apply electronically directly from the course description pages that...

  11. PLACES AVAILABLE

    CERN Multimedia

    Monique DUVAL

    2002-01-01

    Places are available in the following courses: LabView Basics 1 :  4 - 6.3.02  (3 days) CLEAN-2002 : Working in a Clean Room :  7.3.2002  (half day) LabView Base 2 : 11 & 12.3.02 (2 jours) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) Programming the Web for Control Applications : 11, 12, 18, 19.3.2002  (4 * 2 hour lectures) Habilitation électrique : recyclage HT/BT (Français) : 13 - 14.3.2002 (2 * 2 heures) LabView Advanced :  13 - 15.3.02 (3 days) Introduction to the CERN Engineering Data Management System (EDMS) :  20.3.2002  (1 day) The CERN (EDMS) for Advanced Users :  21.3.2002  (1 day) LabVIEW DSC : 25 - 26.4.2002 (2 jours) LabVIEW DAQ : 15 - 16.5.2002 (2 jours) Cours sur la migration AutoCAD :   AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé ...

  12. PLACES AVAILABLE

    CERN Multimedia

    Technical Traininf; Tel. 74924

    2001-01-01

    Places are available in the following courses: Electromagnetic Compatibility (EMC): Introduction (bilingual) :  3.12.01 (half-day) Habilitation électrique : superviseurs : 5.12.01 (1/2 journée) Introduction to the CERN Engineering Data Management System : 07.12.2001 (1 day) LabVIEW - Basics 1 :  10 - 12.12.01 (3 days) Introduction au PC et Windows 2000 : 12 & 14.12.01 (2 jours) LabVIEW - Basics 2 :  13 - 14.12.01 (2 days) Habilitation électrique : superviseurs : 17.12.2001 (1/2 journée) EXCEL 2000 - niveau 2 : 15 - 16.1.02 (2 jours) C++ Programming Level 2 - Traps and Pitfalls :  15 - 18.1.2002  (4 days) Nouveautés de WORD 2000 : 18.1.02 (1/2 journée) LabView hands-on : 21.01.02 (1/2 journée) LabView DAQ hands-on : 21.01.02 (1/2 journée) FileMaker Pro : 22 - 25.1.02 (4 jours) Introduction au PC et à Windows 2000 au CERN : 29 - 30.1....

  13. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : FrontPage 2000 - niveau 1 : 20 & 21.5.03 (2 jours) PIPES-2003 : Pratique du sertissage de tubes métalliques et multicouches : 21.5.03 (1 jour) Introduction à la CAO Cadence : de la saisie de schéma Concept-HDL au PCB : 20 & 22.5.03 (2 jours) AutoCAD 2002 - niveau 2 : 3 & 4.6.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 5, 6, 12, 13, 26, 27.6.03 (6 jours) EXCEL 2000 - niveau 1 : 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) PowerPoint 2000 (F) : 17 & 18.6.03 (2 jours) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) FrontPage 2000 - niveau 2 : 19 & 20.6.03 (2 jours) LabView DSC (langue à décider/language to be defined) : 19 & 20.6.03 EXCEL 2000 - niveau 2 : 24 & 25.6.03 (2 jours) Siemens SIMATIC Training: Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 16 - 20.6.03 (5 days) Simatic...

  14. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : FrontPage 2000 - niveau 1: 20 & 21.5.03 (2 jours) PIPES-2003 : Pratique du sertissage de tubes métalliques et multicouches: 21.5.03 (1 jour) Introduction à la CAO Cadence: de la saisie de schéma Concept-HDL au PCB : 20 & 22.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E): 5, 6, 12, 13, 26, 27.6.03 (6 days) EXCEL 2000 - niveau 1: 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence: 11.6.03 (matin) EXCEL 2000 - level 1: 12 & 13.6.03 (2 days) Introduction to PVSS: 16.6.03 (half-day, pm) Basic PVSS: 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence: 17.6.03 (matin) LabView DSC (language to be defined): 19 & 20.6.03 PVSS - JCOP Framework Tutorial: 20.6.03 (1 day) EXCEL 2000 - niveau 2: 24 & 25.6.03 (2 jours) Siemens SIMATIC Training: Introduction to STEP7: 3 & 4.6.03 (2 days) STEP7 Programming: 16 - 20.6.03 (5 days) Simatic Net Network: 26 & 27.6.03 (2 days) These courses will be given...

  15. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) C++ for Particle Physicists : 8 - 12.10.01 (6 lectures) Cadence Board Design tools : Upgrading to release 14 : 3 1-day sessions on 9, 10 & 11.10.01 MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introd...

  16. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Contract Follow-up (F) : 30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) Nouveautés d'EXCEL : 5.11.01 (1/2 journée) Introduction a Windows 2000 au CERN : 6.11.01 (1/2 journée) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Automates et réseaux de terrain : 13 & 14.11.01 (2 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Autocad Migration support courses: a detail...

  17. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Contract Follow-up (F) : 30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Automates et réseaux de terrain : 13 & 14.11.01 (2 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (3 days) Programmation TSX Premium 2 : 26 ...

  18. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Cadence Board Design tools : Upgrading to release 14 :  3 1-day sessions on 9, 10 & 11.10.01 MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Base 2 : 18 & 19.10.01 (2 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Contract Follow-up (F) :  30.10.01 (1/2 journée) The CERN Engineering Data Management System for Electronics Design :  30.10.01 (1 day) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) LabView Base 1 : 12 - 14.11.01 (3 jours) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2 days) Programming TSX Premium 1 :  19 - 23.11.01  (5 days) Introduction to C Programming :  21- 23.11.01 (3 days) The Java programming language Level 2:  26 - 28.11.01 (...

  19. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction à Windows 2000 au CERN : 2 sessions de _ journée les 24 et 25.9.01 PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) Frontpage 2000 - niveau 1 : 8 et 9.10.01 (2 jours) C++ for Particle Physicists : 8 - 12.10.01 (6 lectures) MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) The Java programming language Level 1: 8 - 9.11.01 (2 days) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to XML :  19 - 20.11.01 (2...

  20. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView DAQ  (F) : 7 & 8.2.02 (2 jours) Hands-on Object-Oriented Design & Programming with Java :  11 - 13.02.02 (3 days) PVSS basics :  18 - 22.2.02 (5 days) Introduction à Windows 2000 : 18.2.02 (1 demi-journée) Introduction to the CERN Engineering Data Management System :  20.2.02 (1 day) Introduction à la CAO CADENCE : 20 & 21.2.02 (2 jours) The CERN Engineering Data Management System for Advanced users :  21.2.02  (1 day) LabView Basics 1 :  4 - 6.3.02  (3 days) Introduction au VHDL et utilisation du simulateur de CADENCE : 6 & 7.3.02 (2 jours) LabView Base 2 : 11 & 12.3.02 (2 jours) C++ for Particle Physicists :  11 - 15.3.2002  (6 * 3 hour lectures) LabView Advanced :  13 - 15.3.02 (3 days) Cours sur la migration AutoCAD :   AutoCAD : Mise à...

  1. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) FrontPage 2000 - niveau 1 : 20...

  2. Places available **

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: PIPES-2003 - Pratique du Sertissage de tubes métalliques et multicouches : 26.8.03 (stage pratique) The CERN Engineering Data Management System (EDMS) for Engineers : 27.8.03 (1 day, free of charge) CLEAN-2002 : Travailler en salle blanche : 4.9.03 (une demi-journée, séminaire gratuit) The CERN Engineering Data Management System (EDMS) for Local Administrators : 24 & 25.9.03 (2 days, free of charge) Siemens SIMATIC Training : Programmation STEP7 - niveau 1 : 29 - 2.10.03 (4 jours) - ouverture des inscriptions fin août Programmation STEP7 - niveau 2 : 13 - 17.10.03 (5 jours) - ouverture des inscriptions fin août Réseau Simatic Net : 22 & 23.10.03 (2 jours) - ouverture des inscriptions fin août CLEAN-2002 : Working in a Cleanroom : 23.20.03 (half day, free of charge) These courses will be given in French or Englis...

  3. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : The CERN EDMS for Local Administrators : 24 & 25.9.03 (2 days, free of charge) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom : 23.10.03 (half day, free of charge) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E) : 17, 18, 24, 25.11 & 1, 2.12.03 (6...

  4. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : EXCEL 2000 - niveau 1 : 10 & 11.6.03 (2 jours) Conception de PCB rapides dans le flot Cadence : 11.6.03 (matin) EXCEL 2000 - level 1 : 12 & 13.6.03 (2 days) Introduction to PVSS : 16.6.03 (p.m.) Basic PVSS : 17 - 19.6.03 (3 days) Réalisation de PCB rapides dans le flot Cadence : 17.6.03 (matin) PVSS - JCOP Framework Tutorial : 20.6.03 (1 day) EXCEL 2000 - niveau 2 : 24 & 25.6.03 (2 jours) Siemens SIMATIC Training : Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) STEP7 Programming : 16 - 20.6.03 (5 jours/5 days) Simatic Net Network : 26 & 27.6.03 (2 jours/2 days) These courses will be given in French or English following the requests. Programmation automate Schneider : Programmation automate Schneider TSX Premium - 1er niveau : 10 - 13.6.03 (4 jours) - audience : toute personne qui veux maitriser la msie en uvre et la programmation d'un automate TSX Premium - objectifs : maitriser la mise en uvre et la programmation d'un autom...

  5. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: CLEAN-2002 : Travailler en salle blanche (séminaire gratuit) : 4.9.03 (une demi-journée) The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours)...

  6. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   Introduction to PVSS (free of charge): 11.11.02  (afternoon) EXCEL 2000 - niveau 1 :  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) AutoCAD 2002 - niveau 1 :  14, 15, 21, 22.11.02  (4 jours) Hands-on Object-Oriented Design and Programming with C++:  19 - 21.11.02  (3 days)  EXCEL 2000 - niveau 2 :  25 & 26.11.02  (2 jours) FrontPage 2000 - niveau 1 :  27 & 28.11.02  (2 jours) December 2002   LabVIEW - DSC (English) :  2 - 3.12.02  (2 days) AutoCAD 2002 - niveau 2 :  2 & 3.12.02  (2 jours) FileMaker (Français) :  2 - 5.12.02  (4 jours) PCAD Schémas - Débutants :  5 & 6.12.02 ...

  7. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Introduction à Windows 2000 au CERN : 2 sessions de _ journée les 24 et 25.9.01 PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PowerPoint 2000 : 1 et 2.10.01 (2 jours) EXCEL 2000 - niveau 1 : 3 et 4.10.01 (2 jours) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) Introduction à Outlook : 5.10.01 (1 journée) Frontpage 2000 - niveau 1 : 8 et 9.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) C++ for Particle Physicists : 8 - 12.10.01 (6 3-hour lectures) MS-Project 2000 - niveau 1 : 15 - 18.10.01 (4 demi-journées) LabView Basics 1 :  15 - 17.10.01  (3 days) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) WORD 2000 : importer et manipuler des images : 19.10.01 (1 journée) Programmation TSX Premium 1 : 22 - 26.10.01...

  8. PLACES AVAILABLE

    CERN Multimedia

    Technical training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PVSS Basics : 20 - 24.8.01 (5 days) PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programming TSX Premium 1: 15 - 19.10.01 (5 days) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 13 - 14.9.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 15 - 17.10.01 (3 jours / 3 days) LabVIEW - Base 2 / LabVIEW - Basics 2 : 18 - 19.10.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 12 - 14.11.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 15 - 16.11.01 (2 jours / 2...

  9. PLACES AVAILABLE

    CERN Multimedia

    Technical training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) Automates et réseaux de terrain : 3 - 4.10.2001 (2 jours) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programmation TSX Premium 1 : 15 - 19.10.01 (5 jours) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Autocad Migration support courses: a detailed calendar will be published shortly for this series of sessions which will start on 15.10.2001. Registration is already open AutoCAD : Mise à jour AutoCAD r-14 vers 2002 (2 jours) AutoCAD Mechanical PowerPack 6 basé sur AutoCAD 2002 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days)...

  10. PLACES AVAILABLES

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: PVSS Basics : 20 - 24.8.01 (5 days) PROFIBUS : 25 - 26.9.01 (2 jours) PROFIBUS : 27 - 28.9.01 (2 days) PCAD Schémas - débutants : 4 - 5.10.01 (2 jours) PCAD PCB - débutants : 8 - 10.10.01 (3 jours) Programming TSX Premium 1: 15 - 19.10.01 (5 days) Programmation TSX Premium 1 : 22 - 26.10.01 (5 jours) Programming TSX Premium 2: 19 - 23.11.01 (5 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) The following LabView courses will be given in either English or French according to demand LabVIEW - Base 1 / LabVIEW - Basics 1 : 10 - 12.9.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 13 - 14.9.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 15 - 17.10.01 (3 jours / 3 days) LabVIEW - Base 2 / LabVIEW - Basics 2 : 18 - 19.10.01 (2 jours / 2 days) LabVIEW - Base 1 / LabVIEW - Basics 1 : 12 - 14.11.01 (3 jours / 3 days) LabVIEW - DAQ / LabVIEW - DAQ : 15 - 16.11.01 (2 jours / 2...

  11. Places available **

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon, ) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6....

  12. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon, ) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6...

  13. Places available **

    CERN Multimedia

    2003-01-01

    Des places sont disponibles dans les cours suivants : Places are available in the following courses : C++ for Particle Physicists : 10 - 14.3.03 (6 X 3-hour lectures) Introduction to PVSS : 10.3.03 (half day, afternoon) Basic PVSS : 11 - 13.3.03 (3 days) PVSS - JCOP Framework Tutorial : 14.3.03 (1 day) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) LabView base 1/LabView Basics 1 : 9 - 11.4.03 (3 jours/3 days) Langue à définir/language to be decided DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 11 & 12.3.03 / 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16...

  14. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : Introduction to PVSS : 10.3.03 (half-day, afternoon) CLEAN-2002 : Working in a cleanroom : 2.4.03 (half-day, afternoon, free course, registration required) LabView Basics 1 : 9 - 11.4.03 (3 days) Language to be decided. DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures). AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03 (6 jours) Siemens SIMATIC Training: Introduction to STEP7 : 3 & 4.6.03 (2 days) STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 days) Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité: Etre TSO au CERN : 3 sessions sont programmées pour 2003 : 25, 26 & 28.3.03 - 24, 25 & 27.6.03 - 4, 5 & 7.11.03 (sessions de 3 jours) ** The number o...

  15. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required) : 2.4.03 (half-day, afternoon) LabView base 1/LabView Basics 1 (Langue à définir/ language to be decided) : 9 - 11.4.03 (3 jours/3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 31.3 - 4.4.03 / 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : 3 sessions sont programmées pour 2003 : 25...

  16. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses : CLEAN-2002 : Working in a cleanroom (free course, registration required): 11.4.03 (half-day, afternoon) LabView Basics 2 : 10 - 11.4.03 (3 days) DISP-2003 - Spring II Term : Advanced Digital Signal Processing : 30.4, 7, 14, 21.5.03 (4 X 2-hour lectures) AutoCAD 2002 - niveau 1 : 29, 30.4 et 7, 8.5.03 (4 jours) Oracle iDS Reports : Build Internet Reports : 5 - 9.5.03 (5 days) AutoCAD 2002 - niveau 2 : 5 & 6.5.03 (2 jours) AutoCAD Mechanical 6 PowerPack (F) : 12, 13, 20, 21, 27 & 28.5.03(6 jours) Formation Siemens SIMATIC /Siemens SIMATIC Training : Introduction à STEP7 /Introduction to STEP7 : 3 & 4.6.03 (2 jours/2 days) Programmation STEP7/STEP7 Programming : 16 - 20.6.03 (5 jours/5 days) Réseau Simatic Net /Simatic Net Network : 15 & 16.4.03 / 26 & 27.6.03 These courses will be given in French or English following the requests. Cours de sécurité : Etre TSO au CERN : Prochaines sessions : 24, 25 & 27.6.03 - 4, 5 & 7....

  17. PLACES AVAILABLE

    CERN Document Server

    Enseignement Technique; Tél. 74924; Technical Training; Monique Duval; Tel. 74924

    2000-01-01

    Places are available in the following courses : Premiers pas avec votre PC 12 - 15.9.00 (4 demi-journées) WORD 20, 21 et 26, 27.9.2000 (4 jours) JAVA programming level 1 25 - 26.9.2000 (2 days) Gaz inflammables 1 26.9.2000 (1 journée) Advanced aspects of PERL 5 6.10.2000 (1 day) Initiation au WWW 10 - 12.10.00 (3 demi-journées) WORD : importer et manipuler des images 16.10.2000 (1 journée) FileMaker 17, 18 et 24, 25.10.00 (4 jours) Nouveautés de WORD 19 et 20.10.2000 (2 jours) ACCESS 1er niveau 30 - 31.10.00 (2 jours)Introduction à PowerPoint 6.11.00 (1 journée)Nouveautés d’EXCEL 7.11.2000(4 demi-journées)Excel 13, 14 et 20, 21.11.00 (4 jours) LabView hands-on 13.11.2000(4 hours)LabView Basics 1 14 - 16.11.2000 (3 days) MS-Project 1er niveau 14-17.11.00 (4 demi-journées) If you wish to participate in one of these courses, please discuss with your supervisor and apply elec...

  18. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView hands-on (bilingue/bilingual): 5.11.02 (matin/morning) LabView DAQ hands-on (bilingue/bilingual):  5.11.02  (après-midi afternoon) Introduction au PC et Windows 2000 au CERN:  6 & 7.11.02  (2 jours) Oracle 8i : Access the Database with Java:  7 & 8.11.02  (2 days) AutoCAD 2002 - niveau 2:  7 & 8.11.02  (2 jours) Introduction to PVSS (free of charge):  11.11.2002 pm  (1/2 day) Basic PVSS:  12 - 14.11.02  (3 days) EXCEL 2000 - niveau 1:  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) LabView Base 1 :  13 - 15.11.02  (3 jours) AutoCAD 2002 - Level 1:  14, 15, 21, 22.11.2002  (4 days) LabVIEW - Advanced:  18 - 20.11.02  (3 days) Auto...

  19. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: LabView Base 1 :  23 - 25.9.02  (3 jours) Object-Oriented Analysis & Design using UML:  25 - 27.9.02  (3 days) LabView DAQ (E):  26 - 27.9.02  (2 days) Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):...

  20. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: November 2002   LabView hands-on (bilingue/bilingual): 5.11.02 (matin/morning) LabView DAQ hands-on (bilingue/bilingual):  5.11.02  (après-midi afternoon) PCAD Schémas - Débutants :  5 & 6.11.02  (2 jours) PCAD PCB - Débutants :  9 - 11.11.02  (3 jours) Introduction au PC et Windows 2000 au CERN :  6 & 7.11.02  (2 jours) Oracle 8i : Access the Database with Java :  7 & 8.11.02  (2 days) Introduction to PVSS (free of charge):  11.11.2002 pm  (1/2 day) Basic PVSS:  12 - 14.11.02  (3 days) EXCEL 2000 - niveau 1 :  12 & 13.11.02  (2 jours) CLEAN-2002: Working in a Cleanroom (English, free of charge):  13.11.2002  (afternoon) LabView Base 1 :  13 - 15.11.02  (3 jours) AutoCAD 2002 - niveau 1 :  14, 15, 21, 22.11.02  (4 jours) LabVIEW - Advanced:  18 - 20.11.02  (3 days) Hands-on Object-Oriented Design and Programming with C++ :  19 - 21.11.02  (3 days)  LabVIEW - Basics 2:  21 - 22.11.02 ...

  1. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Java 2 Enterprise Edition - Part 2: Enterprise JavaBeans:  18 - 20.9.02  (3 days) AutoCAD 2002 - niveau 1 :  19, 20, 26, 27.9.02  (4 jours) LabView Base 1 :  23 - 25.9.02  (3 jours) Object-Oriented Analysis & Design using UML:  25 - 27.9.02  (3 days) LabView DAQ (E):  26 - 27.9.02  (2 days) Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11....

  2. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction à DesignSpace :  16.10.02  (1 journée) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS 21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) LabVIEW Basics 1 (English):  21 - 23.10.02  (3 days) LabVIEW Basics 2 (English):  24 & 25.10.02  (2 days) Oracle 8i : Access the Database with Java:  7 & 8.11.02  (2 days) AutoCAD 2002 - niveau 2 :  7 & 8.11.02  (2 jours) AutoCAD 2002 - Level 1:  14, 15, 21, 22.11.02  (4 days) LabVIEW - Advanced (English) :  18 - 20.11.2002  (3 days) AutoCAD 2002 - niveau 1 :  19, 20, 25, 26.11.02 (4 jours) Oracle iDS Designer: First Class:&...

  3. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) AutoCAD 2002 - niveau 2 :  14 - 15.10.02  (2 jours) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD 2002 - Level 1:  17, 18, 24, 25.10.02  (4 days) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):  21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) HREF-2002: Techniques de la Réfri...

  4. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: PIPES-2003 - Pratique du sertissage de tubes métalliques et multicouches :26.8.03(stage pratique) The CERN EDMS for Engineers (free of charge) : 27.8.03 (1 day) CLEAN-2002 : Travailler en salle blanche (séminaire gratuit) : 4.9.03(une demi-journée) The CERN EDMS for Local Administrators (free of charge) : 24 & 25.9.03 (2 days) HeREF-2003 : Techniques de la réfrigération Hélium (cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom (free of charge) : 23.10.03 (half day) AutoCAD Mechanical 6 PowerPack (E) : 23, 24, 30, 31.10 & 12, 13.11.03 (6 days) AutoCAD 2002 - niveau 2...

  5. PLACES AVAILABLE

    CERN Multimedia

    Monique Duval

    2002-01-01

    Places are available in the following courses: Introduction to Oracle 8i : SQL and PL/SQL:  7 - 11.10.02  (5 days) CLEAN-2002 : Working in a Cleanroom (free of charge):  10.10.02  (half-day, p.m.) LabView Hands-on (bilingue/bilingual) : 10.10.02 (matin/morning) LabView DAQ Hands-on (bilingue/bilingual)  10.10.02 (après-midi /afternoon) Introduction à DesignSpace :  16.10.02  (1 journée) Introduction to DesignSpace:  17.10.02  (1 day) AutoCAD Mechanical 6 PowerPack (F) :  21, 22, 23.10 et 4, 5, 6.11.02  (6 jours) Introduction à ANSYS/Introduction to ANSYS (langue à définir suivant demande/ Language to be chosen according to demand):  21 - 25.10.02  (5 jours/days) HREF-2002: Helium Refrigeration Techniques (English-French, bilingual) :  21 - 25.10.2002  (7 half days) HREF-2002: Techniques de la...

  6. Places available**

    CERN Multimedia

    2003-01-01

    Places are available in the following courses: The CERN EDMS for Local Administrators : 24 & 25.9.03 (2 days, free of charge) HeREF-2003 : Techniques de la réfrigération Hélium cours en français avec support en anglais) : 6 - 10.10.2003 (7 demi-journées) The Java Programming Language Level 1 : 6 - 7.10.2003 (2 days) Java 2 Enterprise Edition - Part 2 : Enterprise JavaBeans : 8 - 10.10.2003 (3 days) FileMaker - niveau 1 : 9 & 10.10.03 (2 jours) EXCEL 2000 - niveau 1 : 20 & 22.10.03 (2 jours) AutoCAD 2002 - niveau 1 : 20, 21, 27, 28.10.03 (4 jours) CLEAN-2002 : Working in a Cleanroom : 23.10.03 (half day, free of charge) AutoCAD 2002 - Level 1 : 3, 4, 12, 13.11.03 (4 days) AutoCAD 2002 - niveau 2 : 10 & 11.11.03 (2 jours) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) AutoCAD Mechanical 6 PowerPack (E) : 17, 18, 24, 25.11 & 1, 2.12.03 (6 days) FrontPage 2000 - niveau 1 : 20 & 21.11.03 (2 jours) MAGNE-03 : Magnétisme pour l'électrotechnique : 25 - 27.11.03 (3 jours) ...

  7. PLACES AVAILABLE

    CERN Multimedia

    Technical Training; Tel. 74924

    2001-01-01

    Places are available in the following courses: Nouveautés d'EXCEL : 5.11.01 (1/2 journée) Introduction a Windows 2000 au CERN : 6.11.01 (1/2 journée) UNIX pour non-programmeurs : 5 - 7.11.01 (3 jours) Design Patterns :  7 - 8.11.01 (2 days) The Java programming language Level 1: 8 - 9.11.01 (2 days) Automates et réseaux de terrain : 13 & 14.11.01 (3 jours) Introduction à Windows 2000 au CERN : 12 - 14.11.01 (1/2 journée) Introduction to Windows 2000 at CERN :  14.11.01  (half-day) Introduction to PERL 5 :  15 - 16.11.01  (2 days) Introduction to C Programming :  21- 23.11.01 (3 days) Programmation TSX Premium 2 : 26 - 30.11.01 (5 jours) Contract Follow-up (F) : 26.11.01 (1/2 journée) Object-Oriented Analysis and Design :  27 - 30.11.2001  (4 days) Hands-on Object-Oriented Design and Programming with C++ :  11 - 13.12.2...

  8. High School Seniors' Career Interests, Educational Plans, and Perceptions of Programs and Services Available at Walla Walla Community College.

    Science.gov (United States)

    VanAusdle, Steven L.; And Others

    In order to develop a marketing program for potential students and assist in institutional planning, a study was conducted at Walla Walla Community College (WWCC), Washington, to obtain information from high school seniors regarding their career interests, educational plans, and perceptions of WWCC's programs and services. Questionnaires were…

  9. [Not Available].

    Science.gov (United States)

    Gutiérrez Avendaño, Jairo

    2016-01-01

    To analyse sociodemographic characteristics, descriptive psychopathology and therapy in the first decade of service of the Hospital Psiquiátrico San Isidro del Valle del Cauca, 1958-1968. Retrospective descriptive study of a series of case histories of the studied period, and by qualitative analysis and conceptual networks. Sociodemographic variables were analysed, and it was observed that, although the patient population increased, hospitalisation was reduced due to the implementation of a "Day Hospital" and outpatient services. The majority of patients were young adults, women engaged in housework, and several lower-middle income patients, primarily referred by their families. Hospital stay was short, with a high frequency of readmissions. The main reason for discharge was improvement, and the predominant diagnosis and symptomatology was schizophrenia, with the treatment being antipsychotic neuroleptics. The correlation of the short time of hospitalisation, more outpatient clinics, the large number of readmissions, and improvement as the reason for discharge, is consistent with the results of the International Pilot Study of Schizophrenia 1968, which included a sample of 100 patients from Hospital psychiatric San Isidro del Valle del Cauca, in which it was concluded that the disorder did not cause a progressive deterioration in patients and that, on the contrary, showed a significant percentage recovery. Copyright © 2015 Asociación Colombiana de Psiquiatría. Publicado por Elsevier España. All rights reserved.

  10. [Not Available].

    Science.gov (United States)

    Palep, Jaydeep H

    2009-01-01

    The term "robot" was coined by the Czech playright Karel Capek in 1921 in his play Rossom's Universal Robots. The word "robot" is from the check word robota which means forced labor. The era of robots in surgery commenced in 1994 when the first AESOP (voice controlled camera holder) prototype robot was used clinically in 1993 and then marketed as the first surgical robot ever in 1994 by the US FDA. Since then many robot prototypes like the Endoassist (Armstrong Healthcare Ltd., High Wycombe, Buck, UK), FIPS endoarm (Karlsruhe Research Center, Karlsruhe, Germany) have been developed to add to the functions of the robot and try and increase its utility. Integrated Surgical Systems (now Intuitive Surgery, Inc.) redesigned the SRI Green Telepresence Surgery system and created the daVinci Surgical System((R)) classified as a master-slave surgical system. It uses true 3-D visualization and EndoWrist((R)). It was approved by FDA in July 2000 for general laparoscopic surgery, in November 2002 for mitral valve repair surgery. The da Vinci robot is currently being used in various fields such as urology, general surgery, gynecology, cardio-thoracic, pediatric and ENT surgery. It provides several advantages to conventional laparoscopy such as 3D vision, motion scaling, intuitive movements, visual immersion and tremor filtration. The advent of robotics has increased the use of minimally invasive surgery among laparoscopically naïve surgeons and expanded the repertoire of experienced surgeons to include more advanced and complex reconstructions.

  11. High-end climate change impact on European water availability and stress: exploring the presence of biases

    Directory of Open Access Journals (Sweden)

    L. V. Papadimitriou

    2015-07-01

    Full Text Available Climate models project a much more substantial warming than the 2 °C target making higher end scenarios increasingly plausible. Freshwater availability under such conditions is a key issue of concern. In this study, an ensemble of Euro-CORDEX projections under RCP8.5 is used to assess the mean and low hydrological states under +4 °C of global warming for the European region. Five major European catchments were analyzed in terms of future drought climatology and the impact of +2 vs. +4 °C global warming was investigated. The effect of bias correction of the climate model outputs and the observations used for this adjustment was also quantified. Projections indicate an intensification of the water cycle at higher levels of warming. Even for areas where the average state may not considerably be affected, low flows are expected to reduce leading to changes in the number of dry days and thus drought climatology. The identified increasing or decreasing runoff trends are substantially intensified when moving from the +2 to the +4 °C of global warming. Bias correction resulted in an improved representation of the historical hydrology. It is also found that the selection of the observational dataset for the application of the bias correction has an impact on the projected signal that could be of the same order of magnitude to the selection of the RCM.

  12. [Not Available].

    Science.gov (United States)

    Abril, Encarnación; Gómez-Conesa, Antonia; Gutiérrez-Santos, Manuel

    2008-05-01

    To assess the quality of physiotherapeutic care in patients treated for low back pain in a Primary Health Care physiotherapy unit, and to improve the quality of the care provided to these patients. The first assessment includes all patients treated in 2002 (n=83). Five criteria corresponding to the initial physiotherapeutic assessment were chosen: C1, pain; C2, disability; C3, mobility, muscle examination, and C5, palpation. In order to detect non-compliance, the Ishikawa Fishbone diagram was used. Corrective measures were established in November 2003: publication of an Oswestry questionnaire model for assessing disability and reflecting on the results obtained. The second assessment includes the period of time from 1st November 2003 to 6th April 2004 (n=32). In the initial assessment, compliances observed were: C1: 21.69%; C2: 0; C3: 69.87%; C4: 78.31%, and C5: 84.33%. After implementing corrective measures a significant improvement was observed in the compliance of C1, C2, and C3. In C4 there was an improvement, and in C5 there was a decrease in compliance, both of them without statistical significance. The carrying out of an improvement cycle has enabled non-compliances in the application of this protocol to be detected and corrected. The corrective measures implemented have led to a reduction of the variability in the records. It is advisable to ensure that compliance does not decrease in the areas that initially showed a high level of compliance. Copyright © 2008 Sociedad Española de Calidad Asistencial. Published by Elsevier Espana. All rights reserved.

  13. [Not Available].

    Science.gov (United States)

    Höck, P

    1994-01-01

    The central region of Switzerland did not participate in the development of psychiatry in the 19th century. It was not before 1909 that the providing for insanes was taken in hand. At that time the capuchin Rufin Steimer founded the first catholic infirmary for mental diseases under the name "Franziskusheim" in the canton of Zug. Brethren of charity came to conduct it. Steimer realized there a project which formerly had seemed impossible. But he was no expert, and his hospital could not be perfect. It was merely a place for taking persons in custody. Because this sanatorium was restricted for men, one had no provision for women until 1927. In this year, the congregation of Sisters of the Holy Cross in Menzingen inaugurated a catholic women's infirmary in an aristocratic villa called "Meisenberg". Both hospitals came under the common direction of professor Johann Baptist Manser. Then they were extended, brought up to date and got modern institutions for treatment. His succesors maintained the high standard. The Franziskusheim as the greater of them is now the psychiatric centre of the cantons of Uri, Schwyz, and Zug. A new building has been opened in March 1994. Both homes are private institutions till now and have preserved their religious background. The gradual rehabilitation of convalescents, the employment of persons with physical or mental defects and the care for sufferings from alcoholism or drugs are great duties of the present time. The above-mentioned cantons have therefore founded places of care and a Service of Social Medicine. There exist now houses for transitory or permanent lodging, special factory-places and particular workshops. These installations, self-help-groups and especially the "Foundation Phoenix" for advice and protection are giving useful and meritorious assistance.

  14. Läkaköha - aktuaalne uurimisteema / Marje Oona

    Index Scriptorium Estoniae

    Oona, Marje, 1963-

    2012-01-01

    TÜ peremeditsiini õppetooli töötajate poolt algatatud uurimistööst, mille eesmärgiks on uurida Bordetella spp. infektsioonide epidemioloogiat, molekulaargeneetikat ja kliinilisi eripärasid ning selgitada läkaköha sagedasema diagnsimise põhjusi Eestis

  15. Läkaköha - aktuaalne uurimisteema / Marje Oona

    Index Scriptorium Estoniae

    Oona, Marje, 1963-

    2012-01-01

    TÜ peremeditsiini õppetooli töötajate poolt algatatud uurimistööst, mille eesmärgiks on uurida Bordetella spp. infektsioonide epidemioloogiat, molekulaargeneetikat ja kliinilisi eripärasid ning selgitada läkaköha sagedasema diagnsimise põhjusi Eestis

  16. Dietary flavonoid fisetin increases abundance of high-molecular-mass hyaluronan conferring resistance to prostate oncogenesis.

    Science.gov (United States)

    Lall, Rahul K; Syed, Deeba N; Khan, Mohammad Imran; Adhami, Vaqar M; Gong, Yuansheng; Lucey, John A; Mukhtar, Hasan

    2016-09-01

    We and others have shown previously that fisetin, a plant flavonoid, has therapeutic potential against many cancer types. Here, we examined the probable mechanism of its action in prostate cancer (PCa) using a global metabolomics approach. HPLC-ESI-MS analysis of tumor xenografts from fisetin-treated animals identified several metabolic targets with hyaluronan (HA) as the most affected. Efficacy of fisetin on HA was then evaluated in vitro and also in vivo in the transgenic TRAMP mouse model of PCa. Size exclusion chromatography-multiangle laser light scattering (SEC-MALS) was performed to analyze the molar mass (Mw) distribution of HA. Fisetin treatment downregulated intracellular and secreted HA levels both in vitro and in vivo Fisetin inhibited HA synthesis and degradation enzymes, which led to cessation of HA synthesis and also repressed the degradation of the available high-molecular-mass (HMM)-HA. SEC-MALS analysis of intact HA fragment size revealed that cells and animals have more abundance of HMM-HA and less of low-molecular-mass (LMM)-HA upon fisetin treatment. Elevated HA levels have been shown to be associated with disease progression in certain cancer types. Biological responses triggered by HA mainly depend on the HA polymer length where HMM-HA represses mitogenic signaling and has anti-inflammatory properties whereas LMM-HA promotes proliferation and inflammation. Similarly, Mw analysis of secreted HA fragment size revealed less HMM-HA is secreted that allowed more HMM-HA to be retained within the cells and tissues. Our findings establish that fisetin is an effective, non-toxic, potent HA synthesis inhibitor, which increases abundance of antiangiogenic HMM-HA and could be used for the management of PCa. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  17. [Not Available].

    Science.gov (United States)

    Carlino, A

    1994-01-01

    Anatomical fugitive sheets are of great interest, as a typographical genre, not only for the history of printing and the history of medicine, but also, more generally, for the history of culture as a whole. They were published as folio woodcuts (more rarely engravings) in Germany, France, Italy, England and the Netherlands from the end of the XVth centkury to the mid XVIIIth century. These sheets contain a figure representing the human body (male and female) and a text describing in a concise and quite rough way the elements of the illustration. What is unusual about these sheets is that the trunk of the figures and some of the internal organs can be lifted up or peeled away, like some contemporary books for children. The figures and some internal parts are very often coloured. Judging from the number of editions (I have found 15 editions published between 1538 and 1540!), anatomical sheets met with a huge commercial success and a very wide circulation indeed. Students of the faculties of medicine and philosophy and barber-surgeons were the more obvious consumers. Students would use them as aids to memory to accompany non-illustrated medical books; barbers to grasp some basic notions of the human body, useful for the practice of phlebotomy. They could be purchased in bookshops, but they were more likely to have been sold in market places or around the University where many colporteurs displayed their cheap prints and booklets. I shall argue, however, that anatomical fugitive sheets have also been produced for and used by a non-professional public, literate but not highly educated, unable to read Latin and more used to learning from images rather than from the written word. A public that was eager to discover the inner secrets of the bodily self, the divine machine that we inhabit. From a scientific point of view anatomical fugitive sheets often reveal errors and differences compared to academic textual and iconographic material on the same subject. The discrepancy

  18. [Not Available].

    Science.gov (United States)

    Spivak, M

    1987-01-01

    Some ideas, whether right or wrong, seem never to die, whatever might be the general evolution of knowledge, of science, or simply of good common sense. This is exactly the case with physical education or sports. For centuries, and especially since the middle of the nineteenth century, its advocates have fought for recognition of its all-round virtues, taken as a concept. But to attain a high level of recognition, not only in society but in official government circles, certain conditions were necessary. Education as a whole, around the middle of the nineteenth century, had to be strict and similar to military drill to be considered efficient, although the French middle classes and bourgeoisie had very little taste for the military. Although they admired physical prowess they had a horror of regimentation. But later on, when the Prussians so easily defeated Austria, feelings of doubt in French superiority began to spread, and a handful of theoreticians of physical exercise tried to show that to combat the so-called degeneration of the race, it was essential to impose a system of education in which physical exercise, coupled with military drill, still had an important place. As scientific arguments they referred to the theories of evolution and its then accepted principle, according to Lamarck, or the transmission of acquired properties and character. Darwin was still unknown in France at that time. These zealous advocates succeeded in convincing the Minister of Public Education, Victor Duruy, to include gymnastics in the normal course of studies in all schools, and by 1869 one can say that a craze for all types of physical exercise and sport had spread into many levels of society. That was the start of a never-ending movement which has passed through various stages of evolution corresponding to the changes in society itself. It has therefore changed its methods, its ways, its forms, but the overall principle remains the same, in spite of the improved understanding

  19. Culture-Independent Metagenomic Surveillance of Commercially Available Probiotics with High-Throughput Next-Generation Sequencing.

    Science.gov (United States)

    Patro, Jennifer N; Ramachandran, Padmini; Barnaba, Tammy; Mammel, Mark K; Lewis, Jada L; Elkins, Christopher A

    2016-01-01

    Millions of people consume dietary supplements either following a doctor's recommendation or at their own discretion to improve their overall health and well-being. This is a rapidly growing trend, with an associated and expanding manufacturing industry to meet the demand for new health-related products. In this study, we examined the contents and microbial viability of several popular probiotic products on the United States market. Culture-independent methods are proving ideal for fast and efficient analysis of foodborne pathogens and their associated microbial communities but may also be relevant for analyzing probiotics containing mixed microbial constituents. These products were subjected to next-generation whole-genome sequencing and analyzed by a custom in-house-developed k-mer counting method to validate manufacturer label information. In addition, the batch variability of respective products was examined to determine if any changes in their formulations and/or the manufacturing process occurred. Overall, the products we tested adhered to the ingredient claims and lot-to-lot differences were minimal. However, there were a few discrepancies in the naming of closely related Lactobacillus and Bifidobacterium species, whereas one product contained an apparent Enterococcus contaminant in two of its three lots. With the microbial contents of the products identified, we used traditional PCR and colony counting methods to comparatively assess our results and verify the viability of the microbes in these products with regard to the labeling claims. Of all the supplements examined, only one was found to be inaccurate in viability. Our use of next-generation sequencing as an analytical tool clearly demonstrated its utility for quickly analyzing commercially available products containing multiple microbes to ensure consumer safety. IMPORTANCE The rapidly growing supplement industry operates without a formal premarket approval process. Consumers rely on product labels to

  20. Detection of highly toxic elements (lead and chromium) in commercially available eyeliner (kohl) using laser induced break down spectroscopy

    Science.gov (United States)

    Gondal, M. A.; Dastageer, M. A.; Al-Adel, F. F.; Naqvi, A. A.; Habibullah, Y. B.

    2015-12-01

    A sensitive laser induced breakdown spectroscopic system was developed and optimized for using it as a sensor for the detection of trace levels of lead and chromium present in the cosmetic eyeliner (kohl) of different price ranges (brands) available in the local market. Kohl is widely used in developing countries for babies as well adults for beautification as well eyes protection. The atomic transition lines at 405.7 nm and 425.4 nm were used as the marker lines for the detection of lead and chromium respectively. The detection system was optimized by finding the appropriate gate delay between the laser excitation and the data acquisition system and also by achieving optically thin plasma near the target by establishing the local thermodynamic equilibrium condition. The detection system was calibrated for these two hazardous elements and the kohl samples under investigation showed 8-15 ppm by mass of lead and 4-9 ppm by mass of Chromium, which are higher than the safe permissible levels of these elements. The limits of detection of the LIBS system for lead and chromium were found to be 1 and 2 ppm respectively.

  1. Variation in bird's originating nitrogen availability limits High Arctic tundra development over last 2000 year (Hornsund, Svalbard)

    Science.gov (United States)

    Skrzypek, Grzegorz; Wojtuń, Bronisław; Hua, Quan; Richter, Dorota; Jakubas, Dariusz; Wojczulanis-Jakubas, Katarzyna; Samecka-Cymerman, Aleksandra

    2016-04-01

    Arctic and subarctic regions play important roles in the global carbon balance. However, nitrogen (N) deficiency is a major constraint for organic carbon sequestration in the High Arctic. Hence, the identification of the relative contributions from different N-sources is critical for understanding the constraints that limit tundra growth. The stable nitrogen composition of the three main N-sources and numerous plants were analyzed in ten tundra types (including those influenced by seabirds) in the Fuglebekken catchment (Hornsund, Svalbard, 77°N 15°E). The percentage of the total tundra N-pool provided by seabirds' feces (from planktivorous colonially breeding little auks Alle alle), ranged from 0-21% in Patterned-ground tundra to 100% in Ornithocoprophilous tundra. The total N-pool utilized by tundra plants in the studied catchment originated from birds (36%), atmospheric deposition (38%), and N2-fixation (26%). The results clearly show that N-pool in the tundra is significantly supplemented by nesting seabirds. Thus, if they experienced climate change induced substantial negative environmental pressure, it would adversely influence the tundra N-budget (Skrzypek et al. 2015). The growth rates and the sediment thickness (PLoS ONE 10(9): e0136536.

  2. Pathophysiologic responses of sheep to brief high level nitrogen dioxide exposure. (Reannouncement with new availability information). Final report

    Energy Technology Data Exchange (ETDEWEB)

    Januszkiewicz, A.J.; Snapper, J.R.; Sturgis, J.W.; Rayburn, D.B.; Dodd, K.T.

    1992-12-31

    The cardiopulmonary response to short-duration, high- concentration nitrogen dioxide (N02) was examined in conscious domestic sheep (Ovis aries). Six intubated animals in each of three groups were administered or approximately 100 or 500 ppm NO2. Pulmonary and systemic hemodynamics, pulmonary mechanics, blood gases and hematologic variables were measured immediately before and after exposure and at 1, 4 and 24 h after exposure. Minute ventilation was monitored during exposure, and a pulmonary histopathologic examination was performed 24 h postexposure. Negligible effects were observed in the control group. Exposure to 100 ppm NO2 caused a modest increase in minute ventilation during exposure, and an increased number of leukocytes were found within interalveolar capillaries upon histologic examination. Exposure to 500 ppm NO2 induced immediate and profound lung irritant responses characterized by increases in lung resistance, respiratory rate and minute ventilation. The exposure was marked by a statistically significant, but small, mixed-venous methemoglobin increase. Pulmonary function progressively deteriorated in the 24-h period following exposure to 500 ppm NO2, and a significant arterial oxygen tension reducting and pulmonary artery pressure increase occurred at 24 h post exposure. Histologic examination after 500 ppm NO2 revealed patchy lobular exudation and accumulation of leukocytes in alveolar sacs and interalveolar capillaries.

  3. ¿Qué ha pasado con el agua en el nuevo estado plurinacional de Bolivia?

    Directory of Open Access Journals (Sweden)

    Nataly Viviana VARGAS GAMBOA

    2015-04-01

    Full Text Available La protección del derecho al agua ha sido el principal objetivo de los más emblemáticos movimientos sociales de los últimos tiempos en Bolivia. Su continua violación ha ocasionado un quiebre en la dinámica social, propiciando un con?icto que ha derivado en el empoderamiento de las clases populares frente a los abusos del gobierno. Dichos movimientos sociales se han movilizado no solo por el cese de la violación puntual del derecho al agua, sino que conscientes de su poder han ido más allá y engendraron el germen de la Asamblea Constituyente, buscando reformar completamente el texto constitucional e incluir en él un amplio espectro de protección para el derecho al agua. Ello ha derivado en el replanteamiento de una serie de políticas por parte del sector público para la realización de este derecho, motivando una fuerte inversión y una gran protección jurisdiccional. Sin embargo, los con?ictos de acceso y protección aún persisten, siendo una gran fuente de preocupación por parte de la población.

  4. Áreas metropolitanas:¿qué ha cambiado?: La experiencia de la Caracas Metropolitana

    Directory of Open Access Journals (Sweden)

    Sonia Barrios

    2001-05-01

    Full Text Available La nueva economía de la información y del conocimiento ha tenido tres decisivas implicaciones de orden territorial. Primero, ha acelerado el proceso de aglomeración urbana a escala planetaria. Segundo, ha provocado fuertes alteraciones en las redes urbanas de ámbito mundial, regional y nacional. Y tercero, ha impulsado la reestructuración interna de las áreas metropolitanas que se habían conformado durante la era industrial. Todo parece anticipar, por lo tanto, polarizaciones y fragmentaciones territoriales todavía más acentuadas que las conocidas en un pasado cercano. Tomando como referencia el caso de Caracas, se trata de averiguar en qué medida el desarrollo reciente de esta metrópoli sigue las grandes tendencias territoriales antes anotadas; y qué esfuerzos se están haciendo para ajustar sus instancias locales a las nuevas realidades urbanas. Como un recurso adicional, en distintos momentos se establecen comparaciones con Barcelona (España, considerada un ejemplo emblemático de las metrópolis de la era de la información

  5. Jom ha-Šo'a...počátky jednoho svátku

    Directory of Open Access Journals (Sweden)

    Zbyněk Tarant

    2011-12-01

    Full Text Available If one looks at the calendar, one will find there 'International Holocaust Rememberance Day' on 27th of January. This day was set by the UN General Assembly in 2005 and it refers to the anniversary of Auschwitz liberation. However, if one looks at the Israeli calendar, or any of the Jewish Luakh haShannah's, one will find there another day of mourning that is actually far older. The Yom HaShoah was set according to the Hebrew calendar and refers not to the liberation of Auschwitz, but to the Warsaw ghetto uprising. Today, this day of mourning is an interesting relict of the pioneer, upbuilding era that preferred the stories of heroes to those of victims. This article attempts to map the very beginning of the Yom HaShoah commemoration, by using Hebrew sources that were never published before in the Czech language. The story of Yom HaShoah in the context of the young state of Israel in the 1950s is an interesting case-study on the issue of how the contemporary political and public discourse affects the collective memory of  future generations.

  6. Single HA2 mutation increases the infectivity and immunogenicity of a live attenuated H5N1 intranasal influenza vaccine candidate lacking NS1.

    Directory of Open Access Journals (Sweden)

    Brigitte M Krenn

    Full Text Available BACKGROUND: H5N1 influenza vaccines, including live intranasal, appear to be relatively less immunogenic compared to seasonal analogs. The main influenza virus surface glycoprotein hemagglutinin (HA of highly pathogenic avian influenza viruses (HPAIV was shown to be more susceptible to acidic pH treatment than that of human or low pathogenic avian influenza viruses. The acidification machinery of the human nasal passageway in response to different irritation factors starts to release protons acidifying the mucosal surface (down to pH of 5.2. We hypothesized that the sensitivity of H5 HA to the acidic environment might be the reason for the low infectivity and immunogenicity of intranasal H5N1 vaccines for mammals. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrate that original human influenza viruses infect primary human nasal epithelial cells at acidic pH (down to 5.4, whereas H5N1 HPAIVs lose infectivity at pH ≤ 5.6. The HA of A/Vietnam/1203/04 was modified by introducing the single substitution HA2 58K→I, decreasing the pH of the HA conformational change. The H5N1 reassortants containing the indicated mutation displayed an increased resistance to acidic pH and high temperature treatment compared to those lacking modification. The mutation ensured a higher viral uptake as shown by immunohistochemistry in the respiratory tract of mice and 25 times lower mouse infectious dose₅₀. Moreover, the reassortants keeping 58K→I mutation designed as a live attenuated vaccine candidate lacking an NS1 gene induced superior systemic and local antibody response after the intranasal immunization of mice. CONCLUSION/SIGNIFICANCE: Our finding suggests that an efficient intranasal vaccination with a live attenuated H5N1 virus may require a certain level of pH and temperature stability of HA in order to achieve an optimal virus uptake by the nasal epithelial cells and induce a sufficient immune response. The pH of the activation of the H5 HA protein may

  7. Role of the HaHOG1 MAP kinase in response of the conifer root and butt rot pathogen (heterobasidion annosum to osmotic and oxidative stress [corrected].

    Directory of Open Access Journals (Sweden)

    Tommaso Raffaello

    Full Text Available The basidiomycete Heterobasidion annosum (Fr. Bref. s.l. is a filamentous white rot fungus, considered to be the most economically important pathogen of conifer trees. Despite the severity of the tree infection, very little is known about the molecular and biochemical aspects related to adaptation to abiotic stresses. In this study, the osmotic and oxidative tolerance as well as the role of the HaHOG1 Mitogen Activated Protein Kinase (MAPK gene were investigated. The transcript levels of the yeast orthologues GPD1, HSP78, STL1, GRE2 and the ATPase pumps ENA1, PMR1, PMC1 known to have an important role in osmotolerance were also quantified under salt osmotic conditions. The HaHOG1 gene was used for a heterologous expression and functional study in the Saccharomyces cerevisiae Δhog1 strain. Moreover, the phosphorylation level of HaHog1p was studied under salt osmotic and oxidative stress. The result showed that H. annosum displayed a decreased growth when exposed to an increased concentration of osmotic and oxidative stressors. GPD1, HSP78, STL1 and GRE2 showed an induction already at 10 min after exposure to salt stress. Among the ATPase pumps studied, PMC1 was highly induced when the fungus was exposed to 0.2 M CaCl₂ for 60 min. The heterologous expression of the HaHOG1 sequence in yeast confirmed that the gene is able to restore the osmotolerance and oxidative tolerance in the S. cerevisiae hog1Δ mutant strain. The HaHog1p was strongly phosphorylated in the presence of NaCl, KCl, H₂O₂ but not in the presence of CaCl₂ and MgCl₂. The GFP-HaHog1p fusion protein accumulated in the nuclei of the S. cerevisiae hog1Δ cells when exposed to high osmotic conditions but not under oxidative stress. These results provide the first insights about the response of H. annosum to osmotic and oxidative stress and elucidate the role of the HaHOG1 gene in such conditions.

  8. SeeHaBITaT: A server on bioinformatics applications for Tospoviruses and other species

    Directory of Open Access Journals (Sweden)

    Seethalakshmi Sakthivel

    2016-06-01

    Full Text Available Plant viruses are important limiting factors in agricultural productivity. Tospovirus is one of the severe plant pathogens, causing damage to economically important food and ornamental crops worldwide through thrips as vectors. Database application resources exclusively on this virus would help to design better control measures, which aren't available. SeeHaBITaT is a unique and exclusive web based server providing work bench to perform computational research on tospoviruses and its species. SeeHaBITaT hosts Tospoviruses specific database Togribase, MOLBIT, SRMBIT and SS with PDB. These applications would be of immense help to the Tospovirus scientific community. The server could be accessed at http://bit.srmuniv.ac.in/.

  9. The Tissue Implant Response Surrounding Subcutaneous TCP, HA, And ALCAP Bioceramics.

    Science.gov (United States)

    Butler, K R; Benghuzzi, Hamed; Tucci, Michelle; Puckett, A D

    2015-01-01

    The objective of this investigation was to quantify and further elucidate the tissue-implant response in the fibrous tissue surrounding tricalcium phosphate (TCP), hydroxyapatite (HA), and aluminum calcium phosphate (ALCAP) implants when implanted subcutaneously. Sixteen animals in four experimental groups (n = 4/group) were implanted with one implant each: Group I (control, TCP), Group II (HA), and Group III (ALCAP). At 90 days post-implantation, the fibrous tissue surrounding the implants was harvested. Sections of stained fibrous tissue were evaluated for the presence of macrophages, fibrocytes, neutrophils, vascularity and thickness for all three groups using semi-automated quantitative methods. The analysis indicated Group III demonstrated a significantly higher number of neutrophils but fewer macrophages and blood vessels per high power field and had a substantially thinner fibrous tissue capsule thickness compared to Groups I and II (alpha=0.05). Group II elicited a greater response of fibroblasts compared to Groups I and III suggesting HA may provide a slightly higher degree of stability to the implant. In total, these findings suggest both TCP and HA behave similarly in vivo when compared to ALCAP and may be better choices for subcutaneous soft-tissue application compared to ALCAP.

  10. The kinematics and binary-induced shaping of PN HaTr 4

    CERN Document Server

    Tyndall, Amy; Lloyd, Myfanwy

    2011-01-01

    We present the first detailed spatio-kinematical analysis of the planetary nebula HaTr 4, one of few known to contain a post-Common-Envelope central star system. Based on high spatial and spectral resolution spectroscopy of the [OIII]5004.84 angstrom nebular emission line, in combination with deep, narrow-band imagery, a spatio-kinematical model was developed in order to accurately determine the three-dimensional morphology and orientation of HaTr 4. The nebula is found to display an extended ovoid morphology with an equatorial enhancement consistent with a toroidal waist - a feature believed to be typical of central star binarity. The nebular inclination is found to be in good agreement with that determined for the binary plane, providing strong evidence that shaping and evolution of HaTr 4 has been influenced by its central binary system - making HaTr 4 one of only 5 planetary nebulae to have had this observationally proven.

  11. Characterization, mechanical properties and corrosion resistance of biocompatible Zn-HA/TiO2 nanocomposite coatings.

    Science.gov (United States)

    Mirak, Mohammad; Alizadeh, Morteza; Ghaffari, Mohammad; Ashtiani, Mohammad Najafi

    2016-09-01

    Biocompatible Zinc-hydroxyapatite-titania and Zinc-hydroxyapatite nanocomposite coatings have been prepared by electrodeposition on NiTi shape memory alloy. Structures of coatings were characterized using X-ray diffraction (XRD). It was found that addition of TiO2 particles cause to reduction of crystallite size of coating. Scanning Electronic Microscope (SEM) observation showed that the Zn-HA/TiO2 coating consists of plate-like regions which can express that this plate-like structure can facilitate bone growth. X-ray photoelectron microscope (XPS) was performed to investigation of chemical state of composite coating and showed that Zinc matrix was bonded to oxygen. high-resolution transmission electron microscope (HRTEM) result illustrated the crystalline structure of nanocomposite coating. Mechanical behavior of coating was evaluated using microhardness and ball on disk wear test. The TiO2 incorporated composite coatings exhibited the better hardness and anti-wear performance than the Zn-HA coatings. Polarization measurements have been used to evaluate the electrochemical coatings performance. The Zn-HA/TiO2 composite coatings showed the highest corrosion resistance compared with Zn-HA and bare NiTi. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Reduced Availability of Sugar-Sweetened Beverages and Diet Soda Has a Limited Impact on Beverage Consumption Patterns in Maine High School Youth

    Science.gov (United States)

    Whatley Blum, Janet E.; Davee, Anne-Marie; Beaudoin, Christina M.; Jenkins, Paul L.; Kaley, Lori A.; Wigand, Debra A.

    2008-01-01

    Objective: To examine change in high school students' beverage consumption patterns pre- and post-intervention of reduced availability of sugar-sweetened beverages (SSB) and diet soda in school food venues. Design: A prospective, quasi-experimental, nonrandomized study design. Setting: Public high schools. Participants: A convenience sample from…

  13. Synopsis of Precision Landing and Hazard Avoidance (PL&HA) Capabilities for Space Exploration

    Science.gov (United States)

    Robertson, Edward A.

    2017-01-01

    Until recently, robotic exploration missions to the Moon, Mars, and other solar system bodies relied upon controlled blind landings. Because terrestrial techniques for terrain relative navigation (TRN) had not yet been evolved to support space exploration, landing dispersions were driven by the capabilities of inertial navigation systems combined with surface relative altimetry and velocimetry. Lacking tight control over the actual landing location, mission success depended on the statistical vetting of candidate landing areas within the predicted landing dispersion ellipse based on orbital reconnaissance data, combined with the ability of the spacecraft to execute a controlled landing in terms of touchdown attitude, attitude rates, and velocity. In addition, the sensors, algorithms, and processing technologies required to perform autonomous hazard detection and avoidance in real time during the landing sequence were not yet available. Over the past decade, NASA has invested substantial resources on the development, integration, and testing of autonomous precision landing and hazard avoidance (PL&HA) capabilities. In addition to substantially improving landing accuracy and safety, these autonomous PL&HA functions also offer access to targets of interest located within more rugged and hazardous terrain. Optical TRN systems are baselined on upcoming robotic landing missions to the Moon and Mars, and NASA JPL is investigating the development of a comprehensive PL&HA system for a Europa lander. These robotic missions will demonstrate and mature PL&HA technologies that are considered essential for future human exploration missions. PL&HA technologies also have applications to rendezvous and docking/berthing with other spacecraft, as well as proximity navigation, contact, and retrieval missions to smaller bodies with microgravity environments, such as asteroids.

  14. Screening of the target genes trans-activated by HLA-HA8 in hepatocytes

    Directory of Open Access Journals (Sweden)

    Qi WANG

    2011-06-01

    Full Text Available Objective To clone and identify the target genes trans-activated by human minor histocompatibility antigen HLA-HA8 in hepatocytes with suppression subtractive hybridization(SSH and bioinfomatics technique.Methods mRNA was isolated from HepG2 cells transfected by pcDNA3.1(--HLA-HA8 and pcDNA3.1(- empty vector,and then used to synthesize the double-stranded cDNA(marked as Tester and Driver,respectively by reverse transcription.After being digested with restriction enzyme Rsa I,the tester cDNA was divided into two parts and ligated to the specific adaptor 1 and adaptor 2,respectively,and then hybridized with driver cDNA twice and underwent PCR twice.The production was subcloned into pEGM-Teasy plasmid vectors to set up the subtractive library.The library was then amplified by transfection into E.coli strain DH5α.The cDNA was sequenced and analyzed in GenBank with Blast search after PCR amplification.Results The subtractive library of genes trans-activated by HLA-HA8 was constructed successfully.The amplified library contained 101 positive clones.Colony PCR showed that all these clones contained 200-1000bp inserts.Twenty eight clones were selected randomly to analyze the sequences.The result of homologous analysis showed that altogether 16 coding sequences were gotten,of which 4 sequences were with unknown function.Conclusions The obtained sequences trans-activated by HLA-HA8 may code different proteins and play important roles in cell growth and metabolism,energy synthesis and metabolism,material transport and signal transduction.This finding will bring some new clues for the studies not only on the biological functions of HLA-HA8,but also on the HBV infection mechanism.

  15. A GENETIC STUDY TO DIFFERENTIAL HA/CA MRSA ISOLATED FROM CLINICAL CASES IN IRAQ HOSPITALS.

    Directory of Open Access Journals (Sweden)

    ISRAA MOHAMED SAFI AL- KADMY

    2013-01-01

    Full Text Available Methicillin-resistant Staphylococcus aureus (MRSA has been a major cause of nosocomial infections since the 1960s . Currently, MRSA is divided into two subgroups: the healthcare associated MRSA (HA-MRSA and community associated MRSA(CA-MRSA, CA-MRSA infections have been increasing. The most common of these infections present in soft skin. The aim of this study to different between CA and HA MRSA in clinical isolates of Baghdad hospitals.Methods: clinical isolates were collected from patients with different infections, Simple laboratory testing followed by the complementary API Staph, followed by antibiotic sensitivity and D-test, and finally by using PCR technique, detection of this genes : mecA, PVL, SCCmec IV and V .Results: A total of 105 S.aureus found 104 methicillin-resistant Staphylococcus aureus (MRSA strains, after a D-test , S.aureus divided to two group: CA and HA –MRSA, where the ratio of CA 18(17.1% out of 105 isolates, while HA reached 87(82.8%. MRSA was characterized by PCR amplification mecA gene, 104(99.04% isolates out of 105 gave positive result, all isolates of HA carry mecA gene, while 17 out of 18 isolates of CA carry mecA gene which was CA-MRSA and one isolates was CA-MSSA . All isolates 18(100% of CA gave positive result in risk factors PVL gene, while for detection of SCCmec IV 17 (94.4% out of 18 isolates of CA gave positive result, and finally two isolates of CA-MRSA gave positive result in SCCmec V gene .Conclusions: This is the first report in Iraq for the emergence of CA isolates especially CA-MRSA which is responsible for the majority of infection in soft tissue and skin abscesses , are likely to be sensitive to clindamycin.

  16. In vitro study of hydroxyapatite/polycaprolactone (HA/PCL) nanocomposite synthesized by an in situ sol-gel process

    Energy Technology Data Exchange (ETDEWEB)

    Rezaei, A.; Mohammadi, M.R., E-mail: mohammadi@sharif.edu

    2013-01-01

    Hydroxyapatite (HA) is the most substantial mineral constituent of a bone which has been extensively used in medicine as implantable materials, owing to its good biocompatibility, bioactivity high osteoconductive, and/or osteoinductive properties. Nevertheless, its mechanical property is not utmost appropriate for a bone substitution. Therefore, a composite consist of HA and a biodegradable polymer is usually prepared to generate an apt bone scaffold. In the present work polycaprolactone (PCL), a newly remarkable biocompatible and biodegradable polymer, was employed as a matrix and hydroxyapatite nanoparticles were used as a reinforcement element of the composite. HA/PCL nanocomposites were synthesized by a new in situ sol-gel process using calcium hydroxide and phosphoric acid precursors in the presence of Tetrahydrofuran (THF) as a solvent. Chemical and physical characteristics of the nanocomposite were studied by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared (FTIR) analyses. The results indicated that pure HA nanoparticles were well-incorporated and homogenously dispersed in the PCL matrix. It was found that the mechanical property of PCL was improved by addition of 20 wt.% HA nanoparticles. Furthermore, the biological property of nanocomposites was investigated under in vitro condition. For this purpose, HA/PCL scaffolds were prepared through a salt leaching process and immersed in a saturated simulated body fluid (SBF) after 3 and 7 days. It was found that a uniform layer of biomimetic HA could be deposited on the surface of HA/PCL scaffolds. Therefore, the prepared HA/PCL scaffolds showed good potential for bone tissue engineering and could be used for many clinical applications in orthopedic and maxillofacial surgery. - Graphical abstract: Preparation of HA/PCL nanocomposite and scaffold by an in situ sol-gel process is shown. Highlights: Black-Right-Pointing-Pointer Hydroxyapatite

  17. PREPARATION OF GENE-VIRAL THERAPEUTIC SYSTEM CNHK200-HA AND ITS ANTITUMOR ACTIVITY ON LUNG CANCER

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objective: To develop a novel adenoviral vector system, which combines the advantages of the antiangiogenic gene therapy and virus therapy, and to investigate its antitumor activity on lung cancer. Methods: A new kind of viral vector CNHK200, in which the E1b55kDa gene was deleted and the whole E1a gene was preserved, was constructed. Human angiostatin gene Kringle 1(5 (hA) was amplified and inserted into the genome of the replicative virus CNHK200, generating CNHK200-hA. The expression of hA and its effect on lung cancer cell growth in vitro and in vivo were studied. Results: The novel vector system CNHK200-hA, just like the replicative virus ONYX-015, replicated in p53-deficient tumor cells but not in normal cells, and thus specifically killed tumor cells. In in vitro experiment, both CNHK200-hA and the non-replicative virus Ad-hA could kill tumor cells but the latter needed 100 times more MOI to achieve the same level of cell killing. In in vivo experiment, the therapeutic effect of CNHK200-hA on human lung cancer A549 xenografts in nude mice was significantly better than that of Ad-hA or that of ONYX-015. Conclusion: CNHK200-hA, which carries the angiostatin gene, has the advantages of specific tumor targeting, high expression of transgene in tumor cells and potent antitumor activity.

  18. Stereo-dynamics of the exchange reaction Ha+LiHb→LiHa+Hb and its isotopic variants

    Institute of Scientific and Technical Information of China (English)

    Zhai Hong-Sheng; Yin Shu-Hui

    2012-01-01

    The quasi-classical trajectory (QCT) method is used to calculate the stereo-dynamics of the exchange reaction Ha+LiHb→LiHa+Hb and its isotopic variants based on an accurate potential energy surface reported by Prudente et al.[Prudente F V,Marques J M C and Maniero A M 2009 Chem.Phys.Lett.474 18].The reactive probability of the title reaction is computed.The vector correlations and four polarization-dependent generalized differential cross sections (PDDCSs) at different collision energies are presented.The influences of the collision energy and the reagent rotation on the product polarization are studied in the present work.The results indicate that the product rotational angular momentum j' is not only aligned,but also oriented along the direction perpendicular to the scattering plane.The product polarization distributions of the title reaction and its isotopic variants exhibit distinct differences which may arise from different mass combinations.

  19. Porous PCL/Chitosan and nHA/PCL/Chitosan Scaffolds for Tissue Engineering Applications: Fabrication and Evaluation

    Directory of Open Access Journals (Sweden)

    Rashid Mad Jin

    2015-01-01

    Full Text Available Two semicrystalline polymers were blended to fabricate porous scaffolds for tissue engineering applications. Scaffolds containing polycaprolactone (PCL/chitosan and nanohydroxyapatite (nHA incorporated nHA/PCL/chitosan were produced using the freeze-drying technique. A model drug, tetracycline hydrochloride (tetracycline HCL, was incorporated into the scaffolds. The scaffolds were characterized using a scanning electron microscope (SEM, EDX, and water contact angle. The antibacterial properties of the nHA/PCL/chitosan/tetracycline HCL scaffold were tested and the scaffolds showed positive results on gram-positive and gram-negative bacteria. The cell biocompatibility using human skin fibroblast cells (HSF 1184 was examined. The scaffold materials were found to be nontoxic to human skin fibroblast cells (HSF 1184 and showed positive proliferation activities. The nHA/PCL/chitosan/tetracycline HCL scaffold has potential for controlling implant-associated bacterial infections during operative procedures and can potentially be used as a scaffold for tissue engineering applications.

  20. Characterization of Palytoxin Binding to HaCaT Cells Using a Monoclonal Anti-Palytoxin Antibody

    Directory of Open Access Journals (Sweden)

    Chiara Florio

    2013-02-01

    Full Text Available Palytoxin (PLTX is the reference compound for a group of potent marine biotoxins, for which the molecular target is Na+/K+-ATPase. Indeed, ouabain (OUA, a potent blocker of the pump, is used to inhibit some PLTX effects in vitro. However, in an effort to explain incomplete inhibition of PLTX cytotoxicity, some studies suggest the possibility of two different binding sites on Na+/K+-ATPase. Hence, this study was performed to characterize PLTX binding to intact HaCaT keratinocytes and to investigate the ability of OUA to compete for this binding. PLTX binding to HaCaT cells was demonstrated by immunocytochemical analysis after 10 min exposure. An anti-PLTX monoclonal antibody-based ELISA showed that the binding was saturable and reversible, with a Kd of 3 × 10−10 M. However, kinetic experiments revealed that PLTX binding dissociation was incomplete, suggesting an additional, OUA-insensitive, PLTX binding site. Competitive experiments suggested that OUA acts as a negative allosteric modulator against high PLTX concentrations (0.3–1.0 × 10−7 M and possibly as a non-competitive antagonist against low PLTX concentrations (0.1–3.0 × 10−9 M. Antagonism was supported by PLTX cytotoxicity inhibition at OUA concentrations that displaced PLTX binding (1 × 10−5 M. However, this inhibition was incomplete, supporting the existence of both OUA-sensitive and -insensitive PLTX binding sites.

  1. The Protecting Effect of Deoxyschisandrin and Schisandrin B on HaCaT Cells against UVB-Induced Damage.

    Directory of Open Access Journals (Sweden)

    Wei Hou

    Full Text Available Schisandra chinensis is a traditional Chinese medicine that has multiple biological activities, including antioxidant, anticancer, tonic, and anti-aging effects. Deoxyschisandrin (SA and schisandrin B (SB, the two major lignans isolated from S. chinensis, exert high antioxidant activities in vitro and in vivo by scavenging free radicals, such as reactive oxygen species (ROS. Ultraviolet B-ray (UVB radiation induces the production of ROS and DNA damage, which eventually leads to cell death by apoptosis. However, it is unknown whether SA or SB protects cells against UVB-induced cellular DNA damage. Our study showed that both SA and SB effectively protected HaCaT cells from UVB-induced cell death by antagonizing UVB-mediated production of ROS and induction of DNA damage. Our results showed that both SA and SB significantly prevented UVB-induced loss of cell viability using 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT assays. Dichloro-dihydro-fluorescein diacetate (DCFH-DA assays showed that the production of ROS following UVB exposure was inhibited by treatment with SA and SB. Moreover, SA and SB decreased the UVB-induced DNA damage in HaCaT cells by comet assays. In addition, SA and SB also prevented UVB-induced cell apoptosis and the cleavage of caspase-3, caspase-8 and caspase-9. In a word, our results imply that the antioxidants SA and SB could protect cells from UVB-induced cell damage via scavenging ROS.

  2. Understanding the Underlying Mechanism of HA-Subtyping in the Level of Physic-Chemical Characteristics of Protein

    Science.gov (United States)

    Ebrahimi, Mansour; Aghagolzadeh, Parisa; Shamabadi, Narges; Tahmasebi, Ahmad; Alsharifi, Mohammed; Adelson, David L.

    2014-01-01

    The evolution of the influenza A virus to increase its host range is a major concern worldwide. Molecular mechanisms of increasing host range are largely unknown. Influenza surface proteins play determining roles in reorganization of host-sialic acid receptors and host range. In an attempt to uncover the physic-chemical attributes which govern HA subtyping, we performed a large scale functional analysis of over 7000 sequences of 16 different HA subtypes. Large number (896) of physic-chemical protein characteristics were calculated for each HA sequence. Then, 10 different attribute weighting algorithms were used to find the key characteristics distinguishing HA subtypes. Furthermore, to discover machine leaning models which can predict HA subtypes, various Decision Tree, Support Vector Machine, Naïve Bayes, and Neural Network models were trained on calculated protein characteristics dataset as well as 10 trimmed datasets generated by attribute weighting algorithms. The prediction accuracies of the machine learning methods were evaluated by 10-fold cross validation. The results highlighted the frequency of Gln (selected by 80% of attribute weighting algorithms), percentage/frequency of Tyr, percentage of Cys, and frequencies of Try and Glu (selected by 70% of attribute weighting algorithms) as the key features that are associated with HA subtyping. Random Forest tree induction algorithm and RBF kernel function of SVM (scaled by grid search) showed high accuracy of 98% in clustering and predicting HA subtypes based on protein attributes. Decision tree models were successful in monitoring the short mutation/reassortment paths by which influenza virus can gain the key protein structure of another HA subtype and increase its host range in a short period of time with less energy consumption. Extracting and mining a large number of amino acid attributes of HA subtypes of influenza A virus through supervised algorithms represent a new avenue for understanding and

  3. Understanding the undelaying mechanism of HA-subtyping in the level of physic-chemical characteristics of protein.

    Science.gov (United States)

    Ebrahimi, Mansour; Aghagolzadeh, Parisa; Shamabadi, Narges; Tahmasebi, Ahmad; Alsharifi, Mohammed; Adelson, David L; Hemmatzadeh, Farhid; Ebrahimie, Esmaeil

    2014-01-01

    The evolution of the influenza A virus to increase its host range is a major concern worldwide. Molecular mechanisms of increasing host range are largely unknown. Influenza surface proteins play determining roles in reorganization of host-sialic acid receptors and host range. In an attempt to uncover the physic-chemical attributes which govern HA subtyping, we performed a large scale functional analysis of over 7000 sequences of 16 different HA subtypes. Large number (896) of physic-chemical protein characteristics were calculated for each HA sequence. Then, 10 different attribute weighting algorithms were used to find the key characteristics distinguishing HA subtypes. Furthermore, to discover machine leaning models which can predict HA subtypes, various Decision Tree, Support Vector Machine, Naïve Bayes, and Neural Network models were trained on calculated protein characteristics dataset as well as 10 trimmed datasets generated by attribute weighting algorithms. The prediction accuracies of the machine learning methods were evaluated by 10-fold cross validation. The results highlighted the frequency of Gln (selected by 80% of attribute weighting algorithms), percentage/frequency of Tyr, percentage of Cys, and frequencies of Try and Glu (selected by 70% of attribute weighting algorithms) as the key features that are associated with HA subtyping. Random Forest tree induction algorithm and RBF kernel function of SVM (scaled by grid search) showed high accuracy of 98% in clustering and predicting HA subtypes based on protein attributes. Decision tree models were successful in monitoring the short mutation/reassortment paths by which influenza virus can gain the key protein structure of another HA subtype and increase its host range in a short period of time with less energy consumption. Extracting and mining a large number of amino acid attributes of HA subtypes of influenza A virus through supervised algorithms represent a new avenue for understanding and

  4. Effects of High Dissolved Inorganic and Organic Carbon Availability on the Physiology of the Hard Coral Acropora millepora from the Great Barrier Reef.

    Directory of Open Access Journals (Sweden)

    Friedrich W Meyer

    Full Text Available Coral reefs are facing major global and local threats due to climate change-induced increases in dissolved inorganic carbon (DIC and because of land-derived increases in organic and inorganic nutrients. Recent research revealed that high availability of labile dissolved organic carbon (DOC negatively affects scleractinian corals. Studies on the interplay of these factors, however, are lacking, but urgently needed to understand coral reef functioning under present and near future conditions. This experimental study investigated the individual and combined effects of ambient and high DIC (pCO2 403 μatm/ pHTotal 8.2 and 996 μatm/pHTotal 7.8 and DOC (added as Glucose 0 and 294 μmol L-1, background DOC concentration of 83 μmol L-1 availability on the physiology (net and gross photosynthesis, respiration, dark and light calcification, and growth of the scleractinian coral Acropora millepora (Ehrenberg, 1834 from the Great Barrier Reef over a 16 day interval. High DIC availability did not affect photosynthesis, respiration and light calcification, but significantly reduced dark calcification and growth by 50 and 23%, respectively. High DOC availability reduced net and gross photosynthesis by 51% and 39%, respectively, but did not affect respiration. DOC addition did not influence calcification, but significantly increased growth by 42%. Combination of high DIC and high DOC availability did not affect photosynthesis, light calcification, respiration or growth, but significantly decreased dark calcification when compared to both controls and DIC treatments. On the ecosystem level, high DIC concentrations may lead to reduced accretion and growth of reefs dominated by Acropora that under elevated DOC concentrations will likely exhibit reduced primary production rates, ultimately leading to loss of hard substrate and reef erosion. It is therefore important to consider the potential impacts of elevated DOC and DIC simultaneously to assess real world

  5. Effect of P Availability on Temporal Dynamics of Carbon Allocation and Glomus intraradices High-Affinity P Transporter Gene Induction in Arbuscular Mycorrhiza

    Science.gov (United States)

    Olsson, Pål Axel; Hansson, Maria C.; Burleigh, Stephen H.

    2006-01-01

    Arbuscular mycorrhizal (AM) fungi depend on a C supply from the plant host and simultaneously provide phosphorus to the colonized plant. We therefore evaluated the influence of external P on C allocation in monoxenic Daucus carota-Glomus intraradices cultures in an AM symbiosis. Fungal hyphae proliferated from a solid minimal medium containing colonized roots into a C-free liquid minimal medium with high or low P availability. Roots and hyphae were harvested periodically, and the flow of C from roots to fungus was measured by isotope labeling. We also measured induction of a G. intraradices high-affinity P transporter to estimate fungal P demand. The prevailing hypothesis is that high P availability reduces mycorrhizal fungal growth, but we found that C flow to the fungus was initially highest at the high P level. Only at later harvests, after 100 days of in vitro culture, were C flow and fungal growth limited at high P availability. Thus, AM fungi can benefit initially from P-enriched environments in terms of plant C allocation. As expected, the P transporter induction was significantly greater at low P availability and greatest in very young mycelia. We found no direct link between C flow to the fungus and the P transporter transcription level, which indicates that a good C supply is not essential for induction of the high-affinity P transporter. We describe a mechanism by which P regulates symbiotic C allocation, and we discuss how this mechanism may have evolved in a competitive environment. PMID:16751522

  6. A positive relationship between harm avoidance and brain nicotinic acetylcholine receptor availability.

    Science.gov (United States)

    Storage, Steven; Mandelkern, Mark A; Phuong, Jonathan; Kozman, Maggie; Neary, Meaghan K; Brody, Arthur L

    2013-12-30

    Prior research indicates that disturbance of cholinergic neurotransmission reduces anxiety, leading to the hypothesis that people with heightened cholinergic function have a greater tendency toward anxiety-like and/or harm-avoidant behavior. We sought to determine if people with elevated levels of harm avoidance (HA), a dimension of temperament from the Temperament and Character Inventory (TCI), have high α4β2* nicotinic acetylcholine receptor (nAChR) availability. Healthy adults (n=105; 47 non-smokers and 58 smokers) underwent bolus-plus-continuous infusion positron emission tomography (PET) scanning using the radiotracer 2-[18F]fluoro-3-(2(S)azetidinylmethoxy) pyridine (abbreviated as 2-FA). During the uptake period of 2-FA, participants completed the TCI. The central study analysis revealed a significant association between total HA and mean nAChR availability, with higher total HA scores being linked with greater nAChR availability. In examining HA subscales, both 'Fear of Uncertainty' and 'Fatigability' were significant, based on higher levels of these characteristics being associated with greater nAChR availabilities. This study adds to a growing body of knowledge concerning the biological basis of personality and may prove useful in understanding the pathophysiology of psychiatric disorders (such as anxiety disorders) that have similar characteristics to HA. Study findings may indicate that heightened cholinergic neurotransmission is associated with increased anxiety-like traits. Published by Elsevier Ireland Ltd.

  7. Marine mud and manure treatment in Ultisols increase pH and phosphate availability and affectCapsicum annum L. grows and production

    Directory of Open Access Journals (Sweden)

    F. Matulessy

    2015-07-01

    Full Text Available Marine mud and manure has potentially to improve ultisol soil condition, especially in soil acidity, CEC, base saturation, neutralizing organic acid, improving soil structure, soil nutrient retention, aeration, soil humidity, capacity of water holding capacity and infiltration and enhance the rapid supply of phosphate for plant grows and development. Two treatments, namely planting media with 200 ton.ha-1 marine mud and 30 ton.ha-1 manure and 400 ton.ha-1 marine mud and 30 ton.ha-1 manure were able to increase pH from 4.6 to 5.6.Significant decrease of Alexcsolubility about 0.03 meq.100 g-1 was found in M1O3; M2O1; M2O3 and M3O1 treatment about. Increase of phosphate about 5.02 ppm was found at treatment 200 ton.ha-1 marine mud and 30 ton.ha-1 manure. There are significant interaction in plant high about 62.42 cm in the media without marine mud and 30 ton.ha-1manure treatments. The amount of 30 ton,ha-1manure produce plant with leaf size about 95,52 cm2.tan-1and produce fresh fruit about 9.81 ton.ha-1.

  8. Effects of High Dissolved Inorganic and Organic Carbon Availability on the Physiology of the Hard Coral Acropora millepora from the Great Barrier Reef

    Science.gov (United States)

    Meyer, Friedrich W.; Vogel, Nikolas; Diele, Karen; Kunzmann, Andreas; Uthicke, Sven; Wild, Christian

    2016-01-01

    Coral reefs are facing major global and local threats due to climate change-induced increases in dissolved inorganic carbon (DIC) and because of land-derived increases in organic and inorganic nutrients. Recent research revealed that high availability of labile dissolved organic carbon (DOC) negatively affects scleractinian corals. Studies on the interplay of these factors, however, are lacking, but urgently needed to understand coral reef functioning under present and near future conditions. This experimental study investigated the individual and combined effects of ambient and high DIC (pCO2 403 μatm/ pHTotal 8.2 and 996 μatm/pHTotal 7.8) and DOC (added as Glucose 0 and 294 μmol L-1, background DOC concentration of 83 μmol L-1) availability on the physiology (net and gross photosynthesis, respiration, dark and light calcification, and growth) of the scleractinian coral Acropora millepora (Ehrenberg, 1834) from the Great Barrier Reef over a 16 day interval. High DIC availability did not affect photosynthesis, respiration and light calcification, but significantly reduced dark calcification and growth by 50 and 23%, respectively. High DOC availability reduced net and gross photosynthesis by 51% and 39%, respectively, but did not affect respiration. DOC addition did not influence calcification, but significantly increased growth by 42%. Combination of high DIC and high DOC availability did not affect photosynthesis, light calcification, respiration or growth, but significantly decreased dark calcification when compared to both controls and DIC treatments. On the ecosystem level, high DIC concentrations may lead to reduced accretion and growth of reefs dominated by Acropora that under elevated DOC concentrations will likely exhibit reduced primary production rates, ultimately leading to loss of hard substrate and reef erosion. It is therefore important to consider the potential impacts of elevated DOC and DIC simultaneously to assess real world scenarios, as

  9. Effects of High Dissolved Inorganic and Organic Carbon Availability on the Physiology of the Hard Coral Acropora millepora from the Great Barrier Reef.

    Science.gov (United States)

    Meyer, Friedrich W; Vogel, Nikolas; Diele, Karen; Kunzmann, Andreas; Uthicke, Sven; Wild, Christian

    2016-01-01

    Coral reefs are facing major global and local threats due to climate change-induced increases in dissolved inorganic carbon (DIC) and because of land-derived increases in organic and inorganic nutrients. Recent research revealed that high availability of labile dissolved organic carbon (DOC) negatively affects scleractinian corals. Studies on the interplay of these factors, however, are lacking, but urgently needed to understand coral reef functioning under present and near future conditions. This experimental study investigated the individual and combined effects of ambient and high DIC (pCO2 403 μatm/ pHTotal 8.2 and 996 μatm/pHTotal 7.8) and DOC (added as Glucose 0 and 294 μmol L-1, background DOC concentration of 83 μmol L-1) availability on the physiology (net and gross photosynthesis, respiration, dark and light calcification, and growth) of the scleractinian coral Acropora millepora (Ehrenberg, 1834) from the Great Barrier Reef over a 16 day interval. High DIC availability did not affect photosynthesis, respiration and light calcification, but significantly reduced dark calcification and growth by 50 and 23%, respectively. High DOC availability reduced net and gross photosynthesis by 51% and 39%, respectively, but did not affect respiration. DOC addition did not influence calcification, but significantly increased growth by 42%. Combination of high DIC and high DOC availability did not affect photosynthesis, light calcification, respiration or growth, but significantly decreased dark calcification when compared to both controls and DIC treatments. On the ecosystem level, high DIC concentrations may lead to reduced accretion and growth of reefs dominated by Acropora that under elevated DOC concentrations will likely exhibit reduced primary production rates, ultimately leading to loss of hard substrate and reef erosion. It is therefore important to consider the potential impacts of elevated DOC and DIC simultaneously to assess real world scenarios, as

  10. The relationship between school characteristics and the availability of mental health and related health services in middle and high schools in the United States.

    Science.gov (United States)

    Slade, Eric P

    2003-01-01

    Problems related to mental illness are increasingly becoming the focal point of public concern over the safety and performance of schools, yet little is known about the availability and quality of school-based mental health services in the United States. In this article it is estimated that approximately 50% of US middle and high schools have any mental health counseling services available onsite and approximately 11% have mental health counseling, physical examinations, and substance abuse counseling available on-site. There are substantial differences in mental health counseling availability by region, urbanicity, and school size, with rural schools, schools in the Midwest and South regions, and small schools being least likely to offer mental health counseling. Multivariate estimates suggest that disparities between schools in the availability of mental health counseling and related health services may be partly explained by differences in access to Medicaid for financing of health services provided at school.

  11. Availability of healthy snack foods and beverages in stores near high-income urban, low-income urban, and rural elementary and middle schools in Oregon.

    Science.gov (United States)

    Findholt, Nancy E; Izumi, Betty T; Nguyen, Thuan; Pickus, Hayley; Chen, Zunqiu

    2014-08-01

    Food stores near schools are an important source of snacks for children. However, few studies have assessed availability of healthy snacks in these settings. The aim of this study was to assess availability of healthy snack foods and beverages in stores near schools and examine how availability of healthy items varied by poverty level of the school and rural-urban location. Food stores were selected based on their proximity to elementary/middle schools in three categories: high-income urban, low-income urban, and rural. Audits were conducted within the stores to assess the presence or absence of 48 items in single-serving sizes, including healthy beverages, healthy snacks, fresh fruits, and fresh vegetables. Overall, availability of healthy snack foods and beverages was low in all stores. However, there was significant cross-site variability in availability of several snack and fruit items, with stores near high-income urban schools having higher availability, compared to stores near low-income urban and/or rural schools. Stores near rural schools generally had the lowest availability, although several fruits were found more often in rural stores than in urban stores. There were no significant differences in availability of healthy beverages and fresh vegetables across sites. Availability of healthy snack foods and beverages was limited in stores near schools, but these limitations were more severe in stores proximal to rural and low-income schools. Given that children frequent these stores to purchase snacks, efforts to increase the availability of healthy products, especially in stores near rural and low-income schools, should be a priority.

  12. Construction of Eukaryotic Expressing Plasmids Encoding HA and HA1 of Influenza A Virus and Their Transient Expression in HEK293 Cells

    Institute of Scientific and Technical Information of China (English)

    ZHANG Weidong; LI Mingyuan; CAO Kang; YANG Jing; SHI Qiaofa; WANG Baoning; JIANG Zhonghua; LI Hong

    2006-01-01

    In order to explore the feasibility and protective efficiency of influenza DNA vaccine, we constructed eukaryotic expressing plasmids encoding HA and HA1 of influenza A virus (A/PR/8/34) and studied their expression in HEK293 cells. HA and HA1 genes were amplified by RT-PCR and cloned into pcDNA3. 1 (+) to generate pcDNA3. 1 (+)/HA and pcDNA3.1 (+)/HA1, respectively. After verification of the cloning fidelity by restriction endonuclease digestion, PCR, and sequencing, pcDNA3.1 (+)/HA and pcDNA3.1 (+)/HA1 were transfected into HEK293 cells using PolyFect Transfection Reagent. Immunofluorescence assay was used to detect the transient expressing cells. Fluorescence microscopy revealed strong expression of target gene in HEK293 cells transiently transfected with either pcDNA3. 1 (+)/HA or pcDNA3. 1 (+)/HA1. Therefore, the results confirm the successful construction of eukaryotic expressing plasmids capable of driving the eukaryotic expression of influenza virus antigen HA and HA1, which is likely to provide a basis for both further investigation of the mechanism of influenza viral infection and the development of influenza DNA vaccine.

  13. Understanding the roles of nanoparticle dispersion and polymer crystallinity in controlling the mechanical properties of HA/PHBV nanocomposites

    Energy Technology Data Exchange (ETDEWEB)

    Noohom, Wadcharawadee; Jack, Kevin S; Martin, Darren; Trau, Matt, E-mail: k.jack@uq.edu.a [Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Queensland 4072 (Australia)

    2009-02-15

    Nano-sized hydroxyapatite (HA) particles stabilized using poly(acrylic acid) (PAA) as a dispersing agent, and sonic energy to further increase dispersion, were blended with poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) using a precipitation/gelation method to produce HA/PHBV nanocomposites with up to 16% by weight of HA content. The level of HA nanoparticle dispersion was monitored in the precursor dispersions prior to composite production and in the nanocomposites by a range of techniques including visual observation, turbidity measurements and electron microscopy, and the roles of the dispersing agent and the sonic energy in controlling the dispersion of HA particles in both the precursor dispersions and the final composites as well as their effects on the compressive strength and Young's modulus were investigated. It was found that HA suspensions treated with both PAA and sonic energy possessed significantly better colloidal stability compared to untreated suspensions or suspensions treated with either PAA or sonic energy. This, in turn, resulted in better dispersion of HA nanoparticles in the composites and higher compressive moduli as a function of the particle loading. This enhancement in stiffness of the composites was attributed primarily to the increased surface area of the HA filler in the more highly dispersed samples, but also to an observed increase in the crystalline content achievable after annealing of the samples. It is proposed that this increase in crystallinity is due to the more highly dispersed particles acting as nucleation sites for the crystallization of the PHBV at the particle interface, which, in turn, leads to enhancement of the bonding between the matrix and filler.

  14. Biomedical potential of chitosan/HA and chitosan/β-1,3-glucan/HA biomaterials as scaffolds for bone regeneration — A comparative study

    Energy Technology Data Exchange (ETDEWEB)

    Przekora, Agata, E-mail: agata.przekora@umlub.pl [Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin (Poland); Palka, Krzysztof [Department of Materials Engineering, Lublin University of Technology, Nadbystrzycka 36, 20-618 Lublin (Poland); Ginalska, Grazyna [Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin (Poland)

    2016-01-01

    The aim of this work was to compare biomedical potential of chitosan/hydroxyapatite (chit/HA) and novel chitosan/β-1,3-glucan/hydroxyapatite (chit/glu/HA) materials as scaffolds for bone regeneration via characterization of their biocompatibility, porosity, mechanical properties, and water uptake behaviour. Biocompatibility of the scaffolds was assessed in direct-contact with the materials using normal human foetal osteoblast cell line. Cytotoxicity and osteoblast proliferation rate were evaluated. Porosity was assessed using computed microtomography analysis and mechanical properties were determined by compression testing. Obtained results demonstrated that chit/HA scaffold possessed significantly better mechanical properties (compressive strength: 1.23 MPa, Young's modulus: 0.46 MPa) than chit/glu/HA material (compressive strength: 0.26 MPa, Young's modulus: 0.25 MPa). However, addition of bacterial β-1,3-glucan to the chit/HA scaffold improved its flexibility and porosity. Moreover, chit/glu/HA scaffold revealed significantly higher water uptake capability (52.6% after 24 h of soaking) compared to the chit/HA (30.7%) and thus can serve as a very good drug delivery carrier. Chit/glu/HA scaffold was also more favourable to osteoblast survival (near 100% viability after 24-h culture), proliferation, and spreading compared to the chit/HA (63% viability). The chit/glu/HA possesses better biomedical potential than chit/HA scaffold. Nevertheless, poor mechanical properties of the chit/glu/HA limit its application to non-load bearing implantation area. - Highlights: • Chitosan/HA and chit/β-1,3-glucan/HA scaffolds for bone regeneration were compared. • Chit/HA significantly reduced osteoblast viability to 63% compared to chit/glu/HA. • Unlike chit/HA, chit/glu/HA favoured cell adhesion, spreading, and proliferation. • Chit/HA had better compressive strength and Young's modulus than chit/glu/HA. • Chit/glu/HA revealed significantly higher

  15. Production of polyclonal antibody against Tehran strain influenza virus (A/H1N1/2009 hemagglutinin conserved domain (HA2: brief report

    Directory of Open Access Journals (Sweden)

    Somayeh Zamani

    2015-10-01

    Full Text Available Background: The influenza virus is one of the most important factors for higher morbidity and mortality in the world. Recently, researchers have been focused on influenza conserved antigenic proteins such as hemagglutinin stalk domain (HA2 for vaccine production and serological studies. The HA2 plays a major role in the fusion of the virus with host cells membrane. The immunity system enables to produce antibody against HA2. The aim of this study is polyclonal antibody production against influenza HA2. Methods: This study was done in the Influenza Research Lab, Pasteur Institute of Iran, Tehran for one year from September 2013 to October 2014. In the present study, recombinant HA2 protein was produced in prokaryotic system and purified using Nickel affinity chromatography. The purified HA2 was mixed with Freund’s adjuvant (complete and incomplete and injected into two New Zealand white rabbits by intramuscularly and subcutaneously routes. Immunization was continued for several months with two weeks interval. Before each immunization, blood was drawn by venous puncture from the rabbit ear. Function of rabbit's sera was evaluated using radial immunodiffusion (RID in both forms, Single RID (SRID and Double RID (DRID. Finally, antiserum activity against HA2 was evaluated using western blotting as serological assay. Results: Sedimentary line and zone was observed in RID assays (SRID and DRID represent interaction between HA2 protein and anti- HA2 antibody. As well as, western blotting results was positive for HA2 protein. Therefore, these results showed that polyclonal antibody produced against HA2 protein can identify HA2 protein antigenic sites. Conclusion: These findings show that humoral immune responses have properly been stimulated in rabbits and these antibodies can identify HA2 protein and may be suitable for other serological methods.

  16. Comparing the Effects of Commercially Available and Custom-Made Video Prompting for Teaching Cooking Skills to High School Students with Autism

    Science.gov (United States)

    Mechling, Linda C.; Ayres, Kevin M.; Foster, Ashley L.; Bryant, Kathryn J.

    2013-01-01

    The study compared the effects of using commercially available and custom-made video prompts on the completion of cooking recipes by four high school age males with a diagnosis of autism. An adapted alternating treatments design with continuous baseline, comparison, final treatment, and best treatment condition was used to compare the two…

  17. High Efficient of the Intermolecular Radical Reactions through three-Component Carbo-Oximation Process using new ready available Sulfonyl oxime.

    Directory of Open Access Journals (Sweden)

    Shireen Mohammed

    2015-09-01

    Full Text Available Addition of functionalized carbon fragments across the olefinic π-system through a free-radical carbo-oximation process offers a straightforward access to valuable intermediates for organic synthesis. For this purpose, we designed a new protected sulfonyl oxime, which enable rapid radical addition with high yields under mild conditions.

  18. Arbuscular mycorrhizal fungi altered the hypericin, pseudohypericin, and hyperforin content in flowers of Hypericum perforatum grown under contrasting P availability in a highly organic substrate.

    Science.gov (United States)

    Lazzara, Silvia; Militello, Marcello; Carrubba, Alessandra; Napoli, Edoardo; Saia, Sergio

    2016-12-20

    St. John's Wort (Hypericum perforatum) is a perennial herb able to produce water-soluble active ingredients (a.i.), mostly in flowers, with a wide range of medicinal and biotechnological uses. However, information about the ability of arbuscular mycorrhizal fungi (AMF) to affect its biomass accumulation, flower production, and concentration of a.i. under contrasting nutrient availability is still scarce. In the present experiment, we evaluated the role of AMF on growth, flower production, and concentration of bioactive secondary metabolites (hypericin, pseudohypericin, and hyperforin) of H. perforatum under contrasting P availability. AMF stimulated the production of aboveground biomass under low P conditions and increased the production of root biomass. AMF almost halved the number of flowers per plant by means of a reduction of the number of flower-bearing stems per plant under high P availability and through a lower number of flowers per stem in the low-P treatment. Flower hyperforin concentration was 17.5% lower in mycorrhizal than in non-mycorrhizal plants. On the contrary, pseudohypericin and hypericin concentrations increased by 166.8 and 279.2%, respectively, with AMF under low P availability, whereas no effect of AMF was found under high P availability. These results have implications for modulating the secondary metabolite production of H. perforatum. However, further studies are needed to evaluate the competition for photosynthates between AMF and flowers at different nutrient availabilities for both plant and AM fungus.

  19. A recombinant vaccine of H5N1 HA1 fused with foldon and human IgG Fc induced complete cross-clade protection against divergent H5N1 viruses.

    Directory of Open Access Journals (Sweden)

    Lanying Du

    Full Text Available Development of effective vaccines to prevent influenza, particularly highly pathogenic avian influenza (HPAI caused by influenza A virus (IAV subtype H5N1, is a challenging goal. In this study, we designed and constructed two recombinant influenza vaccine candidates by fusing hemagglutinin 1 (HA1 fragment of A/Anhui/1/2005(H5N1 to either Fc of human IgG (HA1-Fc or foldon plus Fc (HA1-Fdc, and evaluated their immune responses and cross-protection against divergent strains of H5N1 virus. Results showed that these two recombinant vaccines induced strong immune responses in the vaccinated mice, which specifically reacted with HA1 proteins and an inactivated heterologous H5N1 virus. Both proteins were able to cross-neutralize infections by one homologous strain (clade 2.3 and four heterologous strains belonging to clades 0, 1, and 2.2 of H5N1 pseudoviruses as well as three heterologous strains (clades 0, 1, and 2.3.4 of H5N1 live virus. Importantly, immunization with these two vaccine candidates, especially HA1-Fdc, provided complete cross-clade protection against high-dose lethal challenge of different strains of H5N1 virus covering clade 0, 1, and 2.3.4 in the tested mouse model. This study suggests that the recombinant fusion proteins, particularly HA1-Fdc, could be developed into an efficacious universal H5N1 influenza vaccine, providing cross-protection against infections by divergent strains of highly pathogenic H5N1 virus.

  20. 7 CFR 1945.27 - Relationship between FCIC and FmHA or its successor agency under Public Law 103-354.

    Science.gov (United States)

    2010-01-01

    ... agency under Public Law 103-354. 1945.27 Section 1945.27 Agriculture Regulations of the Department of... under Public Law 103-354. (a) General. Exhibit A of FmHA Instruction 2000-N (available in any FmHA or its successor agency under Public Law 103-354 office) is a Memorandum of Understanding between...

  1. 7 CFR 1945.28 - Relationship between ASCS and FmHA or its successor agency under Public Law 103-354.

    Science.gov (United States)

    2010-01-01

    ... agency under Public Law 103-354. 1945.28 Section 1945.28 Agriculture Regulations of the Department of... under Public Law 103-354. Exhibit A of FmHA Instruction 2000-JJ (a copy of which is available in any FmHA or its successor agency under Public Law 103-354 office) is a Memorandum of Understanding...

  2. 7 CFR 1956.145 - Disposition of essential FmHA or its successor agency under Public Law 103-354 records.

    Science.gov (United States)

    2010-01-01

    ... under Public Law 103-354 records. 1956.145 Section 1956.145 Agriculture Regulations of the Department of... successor agency under Public Law 103-354 records. FmHA or its successor agency under Public Law 103-354 Instruction 2033-A (available in any FmHA or its successor agency under Public Law 103-354 office)...

  3. 流感病毒 HA 受体结合位点抑制剂研究进展%Progress on Inhibitor Agents of Influenza Virus HA Receptor Binding Site

    Institute of Scientific and Technical Information of China (English)

    王文超; 王心竹; 尹荣焕; 赵玉军; 刘娇; 王欣; 李化生; 田菁菁

    2014-01-01

    The anti-flu drugs and flu vaccines were restricted at some time in clinic.So the influenza virus entry inhibitor agents has become the focus of current research.Sialic acid is the receptor of HA,but using sialic acid as viral entry blockers has not been successful,it indicates that sialic acid may not be an ideal scaffold for influenza virus entry inhibitor agents.The receptor binding site of HA must be exposed for binding to host sialic acid receptors,so that the receptor binding site can be monitored by the host immune system,as the potential target binding sites for antibodies.Some antibodies can bind the highly conserved amino acids on the receptor binding site of HA,that inhibits virus infection to hosts.So the antibodies tar-geting binding receptor site of HA may be the new idea for human combat influenza virus.This paper re-viewed the molecular structure and function of the influenza virus HA,mechanism of HA binding the re-ceptors,described the inhibition effect of the antibody to influenza virus entry.in order to supply help for preparation of antibodies inhibiting HA receptor binding site and research influenza virus entry inhibitor.%目前临床所用控制流感病毒的药物和疫苗受到病毒耐药性、疫苗滞后性等诸多因素的限制,使能阻止病毒侵入宿主细胞的抑制剂成为当前研究的热点。唾液酸是流感病毒囊膜表面血凝素(HA)的受体,但研究表明唾液酸并不适合作为研制流感病毒侵入宿主细胞抑制剂的结构模型。研究发现流感病毒的HA 受体结合位点必须外露才能与宿主细胞受体结合,因此 HA 受体结合位点可以被宿主免疫系统监视,成为抗体潜在的靶向契合位点。随后发现的 HA 受体结合位点抗体能与 HA 受体结合位点的保守氨基酸残基结合,能有效阻止流感病毒感染宿主。因此,可用 HA 受体结合位点抗体作为流感病毒感染抑制剂的模型,研制流感病毒抑

  4. The wisconsin Ha mapper: a new look at the warm ionized medium

    Directory of Open Access Journals (Sweden)

    L. M. Haffner

    2000-01-01

    Full Text Available El relevamiento en H-Alpha de Wisconsin (WHAM ha cubierto todo el cielo del norte en H desde Kitt Peak, Arizona. Usando un espectr ometro Fabry-Perot de alta transmisividad, con etal on doble de 15 cm y un CCD de alta sensibilidad, el relevamiento WHAM ha obtenido el primer mapa con velocidades calibradas en H en emisi on de nuestra Galaxia. Una zona amplia, que incluye el brazo Local (Ori on y el brazo de Perseo, tambi en ha sido observada por el WHAM en las l neas de [S II] y [N II]. Estos nuevos datos muestrean directamente y por primera vez, las condiciones f sicas a gran escala del medio ionizado tibio (WIM. Las tendencias de los cocientes de l neas sugieren que las variaciones en la temperatura son muestreadas por los mapas de [N II]/H y [S II]/H . Dado que estos cocientes se incrementan fuertemente en regiones alejadas del plano Gal actico, revelan un incremento sustancial en la temperatura del WIM del halo. Adem as de este resultado, los datos revelan una nueva region H II excitada por una estrella B, dan una medida de la escala de altura de los electrones del WIM y, del cociente [S II]/[N II], proveen nueva informaci on sobre la ionizaci on del WIM.

  5. Glycosylation of Residue 141 of Subtype H7 Influenza A Hemagglutinin (HA) Affects HA-Pseudovirus Infectivity and Sensitivity to Site A Neutralizing Antibodies

    Science.gov (United States)

    Alvarado-Facundo, Esmeralda; Vassell, Russell; Schmeisser, Falko; Weir, Jerry P.; Weiss, Carol D.; Wang, Wei

    2016-01-01

    Human infections with H7 subtype influenza virus have been reported, including an H7N7 outbreak in Netherlands in 2003 and H7N9 infections in China in 2013. Previously, we reported murine monoclonal antibodies (mAbs) that recognize the antigenic site A of H7 hemagglutinin (HA). To better understand protective immunity of H7 vaccines and vaccine candidate selection, we used these mAbs to assess the antigenic relatedness among two H7 HA isolated from past human infections and determine residues that affect susceptibility to neutralization. We found that these mAbs neutralize pseudoviruses bearing HA of A/Shanghai/02/2013(H7N9), but not A/Netherlands/219/2003(H7N7). Glycosylation of the asparagine residue at position 141 (N141) (N133, H3 HA numbering) in the HA of A/Netherlands/219/2003 HA is responsible for this resistance, and it affects the infectivity of HA-pseudoviruses. The presence of threonine at position 143 (T135, H3 HA numbering) in the HA of A/Netherlands/219/2003, rather than an alanine found in the HA of A/Shanghai/02/2013(H7N9), accounts for these differences. These results demonstrate a key role for glycosylation of residue N141 in affecting H7 influenza HA-mediated entry and sensitivity to neutralizing antibodies, which have implications for candidate vaccine design. PMID:26862918

  6. Structure, mechanical property and corrosion behaviors of (HA+β-TCP)/Mg-5Sn composite with interpenetrating networks.

    Science.gov (United States)

    Wang, X; Li, J T; Xie, M Y; Qu, L J; Zhang, P; Li, X L

    2015-11-01

    In this paper, a novel (Hydroxyapatite+β-tricalcium phosphate)/Mg-5Sn ((HA+β-TCP)/Mg-5Sn) composite with interpenetrating networks was fabricated by infiltrating Mg-5Sn alloy into porous HA+β-TCP using suction casting technique. The structure, mechanical property and corrosion behaviors of the composite have been evaluated by means of scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical testing, electrochemical and immersion test. It is shown that the molten Mg-5Sn alloy has infiltrated not only into the pores but also into the struts of the HA+β-TCP scaffold to forming a compact composite. The microstructure observation also shows that the Mg alloy contacts to the HA+β-TCP closely, and no reaction layer can be found between Mg-5Sn alloy and scaffold. The ultimate compressive strength of the composite is as high as 176MPa, which is about four fifths of the strength of the Mg-5Sn bulk alloy. The electrochemical and immersion tests indicate that the corrosion resistance of the composite is better than that of the Mg-5Sn bulk alloy. The corrosion products on the composite surface are mainly Mg(OH)2, Ca3(PO4)2 and HA. Appropriate mechanical and corrosion properties of the (HA+β-TCP)/Mg-5Sn composite indicate its possibility for new bone tissue implant materials.

  7. Putative Phosphorylation Sites On WCA Domain of HA2 Is Essential For Helicoverpa armigera Single Nucleopolyhedrovirus Replication

    Institute of Scientific and Technical Information of China (English)

    Yi-pin Lv; Qian Wang; Chun-chen Wu; Rong Juan Pei; Yuan Zhou; Yun Wang; Xin-wen Chen

    2011-01-01

    Protein phosphorylation is one of the most common post-translational modification processes that play an essential role in regulating protein functionality.The Helicoverpa armigera single nucleopolyhedrovirus (HearNPv) orf2-encoded nucleocapsid protein HA2 participates in orchestration of virus-induced actin polymerization through its WCA domain,in which phosphorylation status are supposed to be critical in respect to actin polymerization.In the present study,two putative phosphorylation sites (232Thr and 250Ser) and a highly conserved Serine (245Ser) on the WCA domain of HA2 were mutated,and their phenotypes were characterized by reintroducing the mutated HA2 into the HearNPV genome.Viral infectivity assays demonstrated that only the recombinant HearNPV bearing HA2 mutation at 245Ser can produce infectious virions,both 232Tbr and 250Ser mutations were lethal to the virus.However,actin polymerization assay demonstrated that all the three viruses bearing HA2 mutations were still capable of initiating actin polymerization in the host nucleus,which indicated the putative phosphorylation sites on HA2 may contribute to HearNPV replication through another unidentified pathway.

  8. Immobilization of alginate-PAC on Sepabeads EC-HA support

    Directory of Open Access Journals (Sweden)

    Žuža Milena G.

    2011-01-01

    Full Text Available Penicillin acylase (PAC is an important industrial enzyme for the production of many β-lactam antibiotics. It is capable of catalyzing the hydrolysis of penicillin G (Pen G to generate phenylacetic acid (PAA and 6-aminopenicillanic acid (6-APA. In this paper, in order to prevent enzyme inactivation, an attempt of coupling enzyme modification and immobilization was presented. Chemical modification was promoted to introduce carbohydrate moiety into the PAC molecule, capable of being covalently linked to an amino support. This seems to provide a possibility to couple the enzyme without risking a reaction at the active site which might cause a loss of activity. PAC molecules were modified by cross-linking with polyaldehyde derivatives of alginate in order to add them new and useful functions. Immobilization of alginate-PAC on Sepabeads EC-HA was used as a model system in order to demonstrate the potential of this strategy. Optimal conditions for covalent immobilization of alginate-PAC from Escherichia coli on support Sepabeads EC-HA, were investigated. The immobilized enzyme was then characterized by evaluating the potential effects of immobilization on its thermal stability, temperature and pH profile in comparison with native non-modified PAC and modified non-immobilized PAC. The maximum amount of the alginate-PAC coupled on the dry support of 99 mg/g was satisfactory. Deactivation rate constants at 50 ºC for free PAC, alginate-PAC and alginate-PAC immobilized on Sepabeads EC-HA were 2,32; 50,65 and 1,68 h-1, respectively. Alginate-PAC and alginate-PAC immobilized on Sepabeads EC-HA had the same pH and temperature optimum as the native non-modified PAC.

  9. Energy at high altitude.

    Science.gov (United States)

    Hill, N E; Stacey, M J; Woods, D R

    2011-03-01

    For the military doctor, an understanding of the metabolic effects of high altitude (HA) exposure is highly relevant. This review examines the acute metabolic challenge and subsequent changes in nutritional homeostasis that occur when troops deploy rapidly to HA. Key factors that impact on metabolism include the hypoxic-hypobaric environment, physical exercise and diet. Expected metabolic changes include augmentation of basal metabolic rate (BMR), decreased availability of oxygen in peripheral metabolic tissues, reduction in VO2 max, increased glucose dependency and lactate accumulation during exercise. The metabolic demands of exercise at HA are crucial. Equivalent activity requires greater effort and more energy than it does at sea level. Soldiers working at HA show high energy expenditure and this may exceed energy intake significantly. Energy intake at HA is affected adversely by reduced availability, reduced appetite and changes in endocrine parameters. Energy imbalance and loss of body water result in weight loss, which is extremely common at HA. Loss of fat predominates over loss of fat-free mass. This state resembles starvation and the preferential primary fuel source shifts from carbohydrate towards fat, reducing performance efficiency. However, these adverse effects can be mitigated by increasing energy intake in association with a high carbohydrate ration. Commanders must ensure that individuals are motivated, educated, strongly encouraged and empowered to meet their energy needs in order to maximise mission-effectiveness.

  10. High Soluble Expression and Immunogenicity Assessment of HA Protein of Avian Influenza Virus%H9N2禽流感病毒HA全长基因的高效可溶表达及免疫原性研究

    Institute of Scientific and Technical Information of China (English)

    谢青梅; 李少璃; 陈丽; 周庆丰; 马静云; 毕英佐; 曹永长

    2006-01-01

    采用RT-PCR方法扩增了H9N2亚型禽流感病毒(AIV)广东株的血凝素基因HA.将HA基因克隆到笔者所设计的原核表达载体pMBX上,成功构建重组表达质粒pMBX-HA.将其转化到E.codi BL-21感受态细胞中表达,经SDS-PAGE可检测到相对分子质量约为96 200的融合蛋白,表达产物占菌体总蛋白的29.5%.经蛋白可溶性分析看出,融合蛋白90%以上是以可溶形式表达的.Western-blot证实,可溶表达的融合蛋白与H9N2 AIV阳性血清具有良好的免疫反应性.将可溶表达的融合蛋白用体积分数为50%的NI-NIA树脂过柱纯化,用融合蛋白作抗原,通过ELISA方法,能特异性地检测出禽流感阳性血清.

  11. Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line

    Energy Technology Data Exchange (ETDEWEB)

    Sandre, C.; Moulin, C.; Bresson, C. [CEA Saclay, DEN, SECR, Lab Speciat Radionucleides and Mol, F-91191 Gif Sur Yvette (France); Gault, N. [CEA Fontenay Roses, DSV IRCM SCSR LRTS, F-92265 Fontenay Aux Roses (France); Poncy, J. L. [CEA Bruyeres Le Chatel, DSV IRCM SREIT LRT, F-91680 Bruyeres Le Chatel (France); Lefaix, J. L. [CEA Caen, DSV IRCM SRO LARIA, F-14070 Caen (France)

    2010-07-01

    Cobalt (Co) is an essential trace element well known as a constituent of vitamin B{sub 12}, but different compounds of Co are also described as highly toxic and/or radio-toxic for individuals or the environment. In nuclear power plants, {sup 58}Co and {sup 60}Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl{sub 2}) with or without gamma-ray doses to mimic contamination by {sup 60}Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate gamma-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species. (authors)

  12. Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line

    Energy Technology Data Exchange (ETDEWEB)

    Gault, N. [CEA Fontenay aux Roses, DSV/IRCM/SCSR/LRTS, 92265 Fontenay aux Rose (France); Sandre, C.; Moulin, B.; Bresson, C. [CEA, DEN, SECR, Laboratoire de Speciation des Radionucleides et des Molecules, F-91191 Gif-sur-Yvette (France); Poncy, J.L. [CEA Bruyeres Le Chatel, DSV/IRCM/SREIT/LRT, 91680 Bruyeres Le Chatel (France); Lefaix, J.L. [CEA Caen, DSV/IRCM/SRO/LARIA, 14070 Caen (France)

    2010-07-01

    Cobalt (Co) is an essential trace element well known as a constituent of vitamin B12, but different compounds of Co are also described as highly toxic and/or radio-toxic for individuals or the environment. In nuclear power plants, {sup 58}Co and {sup 60}Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl{sub 2}) with or without {gamma}-ray doses to mimic contamination by {sup 60}Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate {gamma}-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species. (authors)

  13. The hormesis effect of plasma-elevated intracellular ROS on HaCaT cells

    Science.gov (United States)

    Szili, Endre J.; Harding, Frances J.; Hong, Sung-Ha; Herrmann, Franziska; Voelcker, Nicolas H.; Short, Robert D.

    2015-12-01

    We have examined the link between ionized-gas plasma delivery of reactive oxygen species (ROS) to immortalized keratinocyte (HaCaT) cells and cell fate, defined in terms of cell viability versus death. Phospholipid vesicles were used as cell mimics to measure the possible intracellular ROS concentration, [ROSi], delivered by various plasma treatments. Cells were exposed to a helium cold atmospheric plasma (CAP) jet for different plasma exposure times (5-60 s) and gas flow rates (50-1000 ml min-1). Based upon the [ROSi] data we argue that plasma-generated ROS in the cell culture medium can readily diffuse into real cells. Plasma exposure that equated to an [ROSi] in the range of 3.81  ×  10-10-9.47  ×  10-8 M, measured at 1 h after the plasma exposure, resulted in increased cell viability at 72 h; whereas a higher [ROSi] at 1 h decreased cell viability after 72 h of culture. This may be because of the manner in which the ROS are delivered by the plasma: HaCaT cells better tolerate a low ROS flux over an extended plasma exposure period of 1 min, compared to a high flux delivered in a few seconds, although the final [ROSi] may be the same. Our results suggest that plasma stimulation of HaCaT cells follows the principle of hormesis.

  14. Fabrication and bioactivity behavior of HA/bioactive glass composites in the presence of calcium hexaboride

    Energy Technology Data Exchange (ETDEWEB)

    El-Bassyouni, Gehan T.; Beherei, Hanan H. [Biomaterials Dept., National Research Centre (NRC), Dokki, Cairo (Egypt); Mohamed, Khaled R., E-mail: kh_rezk1966@yahoo.com [Biomaterials Dept., National Research Centre (NRC), Dokki, Cairo (Egypt); Kenawy, Sayed H. [Ceramics Dept., National Research Centre (NRC), Dokki, Cairo (Egypt)

    2016-06-01

    In the current study, composites were prepared using both the synthesized nano-sized hydroxyapatite (HA), bioactive glass (BG) powders (obtained by the traditional melt-quenching route) together with the purchased nano-sized calcium hexaboride (CB) with different ratios and were fired at 1250 °C. The structure and composition of the solid reaction products were analyzed using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy; scanning electron microscope (SEM) coupled with energy dispersive spectroscopy (EDS), transmission electron microscope (TEM) techniques and compressive strength. The mechanical testing was to designate the role of the CB in improving the mechanical property of the prepared composites. In vitro bioactivity of the prepared composites was assessed by soaking in the simulated body fluid (SBF) at 37 ± 0.5 °°C for 10 days. The effect of different ratios of the three components (CB, HA & BG) on the bioactivity properties was assessed to explore the possibility of enhancing such property to perform in vitro imitations of in vivo conditions in the future. It can be pointed out that the Si-HA content in the composition showed outstanding in vitro bioactivity than pure hydroxyapatite which could be attributed to the excellent bioactivity of the synthesized composites. - Highlights: • The prepared of nano-composites containing CB, HA and BG powders were achieved. • The addition of CB powder enhanced the compressive strength for all the composites. • The composites containing high BG and CB contents improved formation of bone-like apatite layer.

  15. HDPE/HA composites obtained in solution: Effect of the gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Carmen, Albano [Polymer Laboratory, Chemistry Center, and Materials Laboratory, Technology Center, Venezuelan Institute for Scientific Research (IVIC), P.O. Box 21827, Caracas 1020-A (Venezuela) and Universidad Central de Venezuela, Facultad de Ingenieria, Escuela de Ingenieria Quimica, Caracas (Venezuela)]. E-mail: calbano@ivic.ve; Arquimedes, Karam [Polymer Laboratory, Chemistry Center, and Materials Laboratory, Technology Center, Venezuelan Institute for Scientific Research (IVIC), P.O. Box 21827, Caracas 1020-A (Venezuela)]. E-mail: akaram@quimica.ivic.ve; Rosestela, Perera [Universidad Simon Bolivar, Departamento de Mecanica, Caracas (Venezuela); Gema, Gonzalez [Polymer Laboratory, Chemistry Center, and Materials Laboratory, Technology Center, Venezuelan Institute for Scientific Research (IVIC), P.O. Box 21827, Caracas 1020-A (Venezuela); Nohemy, Dominguez [Polymer Laboratory, Chemistry Center, and Materials Laboratory, Technology Center, Venezuelan Institute for Scientific Research (IVIC), P.O. Box 21827, Caracas 1020-A (Venezuela); Jeanette, Gonzalez [Universidad Simon Bolivar, Departamento de Mecanica, Caracas (Venezuela); Yanixia, Sanchez [Polymer Laboratory, Chemistry Center, and Materials Laboratory, Technology Center, Venezuelan Institute for Scientific Research (IVIC), P.O. Box 21827, Caracas 1020-A (Venezuela)

    2006-06-15

    Radiation is employed to sterilize composite materials used in the biomedical field. Due to the changes induced by radiation onto polymeric materials, it is important to study variations in their melt flow index (MFI), as well as in their mechanical and thermal properties. In this work, those previous parameters were determined in composites obtained via solution of a high-density polyethylene (HDPE) in decalin, with different amounts of hydroxyapatite (HA), varying from 10 to 30 parts per hundred, after being exposed to gamma radiation at absorbed doses between 25 and 100 kGy. After the irradiation, the MFI of HDPE dissolved in decalin and precipitated afterwards and without filler increased from 6 to 24 g/10 min at the highest absorbed doses. This behavior was also observed in composites with 10 pph of HA, being the increase less pronounced, specifically in the range between 50 and 100 kGy. Composites with 20 and 30 pph of HA showed a maximum MFI value at 50 kGy, which decreased at higher doses. This implies that the filler begin to exert an influence because it does not melt at the test temperature and consequently, it does not flow. It was observed that Young's modulus increased with HA addition due to rigidity of the ceramic filler. Radiation did not significantly affect this tensile property. On the other hand, the tensile strength did not show significant variations at the different doses but the filler content did affect this property improving it. Finally, elongation at break showed a drastic decrease with filler addition. When the thermal behavior was studied it was noticed that crystallization and melting temperatures remained unchanged. Instead, crystallinity degree slightly increased in the composites, and a little decrease was obtained when they were irradiated.

  16. High-flow nasal oxygen availability for sedation decreases the use of general anesthesia during endoscopic retrograde cholangiopancreatography and endoscopic ultrasound

    Science.gov (United States)

    Schumann, Roman; Natov, Nikola S; Rocuts-Martinez, Klifford A; Finkelman, Matthew D; Phan, Tom V; Hegde, Sanjay R; Knapp, Robert M

    2016-01-01

    AIM To examine whether high-flow nasal oxygen (HFNO) availability influences the use of general anesthesia (GA) in patients undergoing endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic ultrasound (EUS) and associated outcomes. METHODS In this retrospective study, patients were stratified into 3 eras between October 1, 2013 and June 30, 2014 based on HFNO availability for deep sedation at the time of their endoscopy. During the first and last 3-mo eras (era 1 and 3), no HFNO was available, whereas it was an option during the second 3-mo era (era 2). The primary outcome was the percent utilization of GA vs deep sedation in each period. Secondary outcomes included oxygen saturation nadir during sedation between periods, as well as procedure duration, and anesthesia-only time between periods and for GA vs sedation cases respectively. RESULTS During the study period 238 ERCP or EUS cases were identified for analysis. Statistical testing was employed and a P sedation was significantly higher in era 2 compared to era 3 (P sedation (P ≤ 0.007) as was the anesthesia-only time (P ≤ 0.001). CONCLUSION High-flow nasal oxygen availability was associated with decreased GA utilization and improved oxygenation for ERCP and EUS during sedation. PMID:28058020

  17. Impact of Shrimp Farm Effluent on Water Quality in Coastal Areas of the World Heritage-Listed Ha Long Bay

    Directory of Open Access Journals (Sweden)

    Thuyet D. Bui

    2012-01-01

    Full Text Available Problem statement: Shrimp farming has rapidly developed in coastal areas of the World Heritage-listed Ha Long Bay since the last decade. Effluent discharged from shrimp farms with high levels of nutrient waste may cause eutrophication in receiving waterways. Therefore, assessing water quality at tidal creeks receiving shrimp farm effluent in coastal areas of Ha Long Bay supports environmental protection and decision making for sustainable development of the region. Approach: Water samples were collected at 3 different locations for spatial assessment: inside sections of creeks directly receiving farm effluent (IEC, from main creeks adjacent to points of effluent discharge outside concentrated shrimp farms (OEC and a few kilometers away from shrimp farm (ASF. Samples were taken on 3 occasions for temporal assessment. Parameters related to nutrient waste from shrimp farms, including: Total Ammonia Nitrogen (TAN, Nitrite-Nitrogen (NO2-N, Nitrate-Nitrogen (NO3-N, Total Phosphorus (TP, Dissolved Orthophosphate (PO4-P, Biochemical Oxygen Demand (BOD, Chemical Oxygen Demand (COD, Total Suspended Solid (TSS, Chlorophyll-a (Chl-a, Temperature, Salinity, pH and Dissolved Oxygen (DO were determined using standard methods. Results: There were statistically significant differences in the concentrations of TAN, NO2-N, NO3-N, TP, PO4-P, BOD, COD and Chl-a among IEC, OEC, ASF and the levels of these parameters increased after shrimp crops, especially after the main shrimp crop of the season in North Vietnam. The concentrations of TAN, NO3-N, TP, BOD, COD, Chl-a, TSS at IEC sites were higher than recommended for protecting aquatic ecosystems. Principal Component Analysis (PCA efficiently summarized patterns of co-variation in water quality parameters among locations and study times. Conclusion/Recommendations: The findings of this study indicate that greater awareness of the environmental impacts of shrimp farms is required if this industry is to be sustainable

  18. “Sono i [meta]dati, stupido”: perché Elsevier ha comprato SSRN

    Directory of Open Access Journals (Sweden)

    Maria Chiara Pievatolo

    2016-05-01

    Full Text Available Mentre molti autori temono che i testi da loro depositati in SSRN vengano resi meno accessibili dopo il suo acquisto da parte di Elsevier, il rischio più serio non è questo. Elsevier, infatti, grazie a SSRN, e alla possibilità di connetterlo a Mendeley, di cui si era già impadronita in precedenza, metterà le mani su un'enorme quantità di metadati. Questo articolo perché questo è un pericolo per chi ha a cuore l'autonomia della ricerca.

  19. Detection of multiple H3 receptor affinity states utilizing [3H]A-349821, a novel, selective, non-imidazole histamine H3 receptor inverse agonist radioligand.

    Science.gov (United States)

    Witte, David G; Yao, Betty Bei; Miller, Thomas R; Carr, Tracy L; Cassar, Steven; Sharma, Rahul; Faghih, Ramin; Surber, Bruce W; Esbenshade, Timothy A; Hancock, Arthur A; Krueger, Kathleen M

    2006-07-01

    1. A-349821 is a selective histamine H3 receptor antagonist/inverse agonist. Herein, binding of the novel non-imidazole H3 receptor radioligand [3H]A-349821 to membranes expressing native or recombinant H3 receptors from rat or human sources was characterized and compared with the binding of the agonist [3H]N--methylhistamine ([3H]NMH). 2. [3H]A-349821 bound with high affinity and specificity to an apparent single class of saturable sites and recognized human H3 receptors with 10-fold higher affinity compared to rat H3 receptors. [3H]A-349821 detected larger populations of receptors compared to [3H]NMH. 3. Displacement of [3H]A-349821 binding by H3 receptor antagonists/inverse agonists was monophasic, suggesting recognition of a single binding site, while that of H3 receptor agonists was biphasic, suggesting recognition of both high- and low-affinity H3 receptor sites. 4. pKi values of high-affinity binding sites for H3 receptor competitors utilizing [3H]A-349821 were highly correlated with pKi values obtained with [3H]NalphaMH, consistent with labelling of H3 receptors by [3H]A-349821. 5. Unlike assays utilizing [3H]NMH, addition of GDP had no effect on saturation parameters measured with [3H]A-349821, while displacement of [3H]A-349821 binding by the H3 receptor agonist histamine was sensitive to GDP. 6. In conclusion, [3H]A-349821 labels interconvertible high- and low-affinity states of the H3 receptor, and displays improved selectivity over imidazole-containing H3 receptor antagonist radioligands. [3H]A-349821 competition studies showed significant differences in the proportions and potencies of high- and low-affinity sites across species, providing new information about the fundamental pharmacological nature of H3 receptors.

  20. Central serotonin transporter availability in highly obese individuals compared with non-obese controls: A [{sup 11}C] DASB positron emission tomography study

    Energy Technology Data Exchange (ETDEWEB)

    Hesse, Swen; Sabri, Osama [University of Leipzig, Department of Nuclear Medicine, Leipzig (Germany); Integrated Research and Treatment Centre Adiposity Diseases Leipzig, Leipzig (Germany); Rullmann, Michael [University of Leipzig, Department of Nuclear Medicine, Leipzig (Germany); Integrated Research and Treatment Centre Adiposity Diseases Leipzig, Leipzig (Germany); Max Planck Institute for Human Cognitive and Brain Sciences Leipzig, Leipzig (Germany); Luthardt, Julia; Becker, Georg-Alexander; Bresch, Anke; Patt, Marianne; Meyer, Philipp M. [University of Leipzig, Department of Nuclear Medicine, Leipzig (Germany); Winter, Karsten [University of Leipzig, Centre for Translational Regenerative Medicine, Leipzig (Germany); University of Leipzig, Institute for Medical Informatics, Statistics, and Epidemiology, Leipzig (Germany); Hankir, Mohammed K.; Zientek, Franziska; Reissig, Georg; Drabe, Mandy [Integrated Research and Treatment Centre Adiposity Diseases Leipzig, Leipzig (Germany); Regenthal, Ralf [University of Leipzig, Division of Clinical Pharmacology, Rudolf Boehm Institute of Pharmacology and Toxicology, Leipzig (Germany); Schinke, Christian [University of Leipzig, Department of Neurology, Leipzig (Germany); Arelin, Katrin [Max Planck Institute for Human Cognitive and Brain Sciences Leipzig, Leipzig (Germany); University of Leipzig, Day Clinic for Cognitive Neurology, Leipzig (Germany); Lobsien, Donald [University of Leipzig, Department of Neuroradiology, Leipzig (Germany); Fasshauer, Mathias; Fenske, Wiebke K.; Stumvoll, Michael [Integrated Research and Treatment Centre Adiposity Diseases Leipzig, Leipzig (Germany); University of Leipzig, Medical Department III, Leipzig (Germany); Blueher, Matthias [University of Leipzig, Medical Department III, Leipzig (Germany); University of Leipzig, Collaborative Research Centre 1052 Obesity Mechanisms, Leipzig (Germany)

    2016-06-15

    The role of the central serotonin (5-hydroxytryptamine, 5-HT) system in feeding has been extensively studied in animals with the 5-HT family of transporters (5-HTT) being identified as key molecules in the regulation of satiety and body weight. Aberrant 5-HT transmission has been implicated in the pathogenesis of human obesity by in vivo positron emission tomography (PET) and single-photon emission computed tomography (SPECT) imaging techniques. However, results obtained thus far from studies of central 5-HTT availability have been inconsistent, which is thought to be brought about mainly by the low number of individuals with a high body mass index (BMI) previously used. The aim of this study was therefore to assess 5-HTT availability in the brains of highly obese otherwise healthy individuals compared with non-obese healthy controls. We performed PET using the 5-HTT selective radiotracer [{sup 11}C] DASB on 30 highly obese (BMI range between 35 and 55 kg/m{sup 2}) and 15 age- and sex-matched non-obese volunteers (BMI range between 19 and 27 kg/m{sup 2}) in a cross-sectional study design. The 5-HTT binding potential (BP{sub ND}) was used as the outcome parameter. On a group level, there was no significant difference in 5-HTT BP{sub ND} in various cortical and subcortical regions in individuals with the highest BMI compared with non-obese controls, while statistical models showed minor effects of age, sex, and the degree of depression on 5-HTT BP{sub ND}. The overall finding of a lack of significantly altered 5-HTT availability together with its high variance in obese individuals justifies the investigation of individual behavioral responses to external and internal cues which may further define distinct phenotypes and subgroups in human obesity. (orig.)

  1. Differences in home food availability of high- and low-fat foods after a behavioral weight control program are regional not racial

    Directory of Open Access Journals (Sweden)

    West Delia

    2010-09-01

    Full Text Available Abstract Background Few studies, if any, have examined the impact of a weight control program on the home food environment in a diverse sample of adults. Understanding and changing the availability of certain foods in the home and food storage practices may be important for creating healthier home food environments and supporting effective weight management. Methods Overweight adults (n = 90; 27% African American enrolled in a 6-month behavioral weight loss program in Vermont and Arkansas. Participants were weighed and completed measures of household food availability and food storage practices at baseline and post-treatment. We examined baseline differences and changes in high-fat food availability, low-fat food availability and the storage of foods in easily visible locations, overall and by race (African American or white participants and region (Arkansas or Vermont. Results At post-treatment, the sample as a whole reported storing significantly fewer foods in visible locations around the house (-0.5 ± 2.3 foods, with no significant group differences. Both Arkansas African Americans (-1.8 ± 2.4 foods and Arkansas white participants (-1.8 ± 2.6 foods reported significantly greater reductions in the mean number of high-fat food items available in their homes post-treatment compared to Vermont white participants (-0.5 ± 1.3 foods, likely reflecting fewer high-fat foods reported in Vermont households at baseline. Arkansas African Americans lost significantly less weight (-3.6 ± 4.1 kg than Vermont white participants (-8.3 ± 6.8 kg, while Arkansas white participants did not differ significantly from either group in weight loss (-6.2 ± 6.0 kg. However, home food environment changes were not associated with weight changes in this study. Conclusions Understanding the home food environment and how best to measure it may be useful for both obesity treatment and understanding patterns of obesity prevalence and health disparity.

  2. Differential Effect of Isooctane Doses on HaCaT and HeLa: A Multimodal Analysis

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    Lopamudra Das

    2014-01-01

    Full Text Available A multimodal approach is effective in analyzing biological problems critically and thus also useful in assessing cytotoxicity under chemicals assaults. In this study effects of isooctane, an organic solvent and component of gasoline produced in petroleum industries, have been explored on normal (HaCaT and cancerous (HeLa epithelial cells. Besides morphological alterations, impacts on viability, prime molecular expressions, and bioelectrical properties on exposure to different doses of isooctane were noted. Scanning electron microscopy and viability assay demonstrated remarkable structural alterations and cell death, respectively, in HaCaT but not in HeLa. Transcriptomic and immunocytochemical studies on E-cadherin expression also elucidated pronounced toxic effects on HaCaT. Remarkable changes on the bioelectrical properties (e.g., impedance and phase angle of the HaCaT, in contrast to HeLa, at different temporal points on isooctane exposure also indicated cytotoxic effects in the former. Hence this study illustrated cytotoxicity of isooctane on HaCaT multidimensionally which was evaded by HeLa.

  3. The Study on Biocompatibility of Porous nHA/PLGA Composite Scaffolds for Tissue Engineering with Rabbit Chondrocytes In Vitro

    Directory of Open Access Journals (Sweden)

    Lei Chen

    2013-01-01

    Full Text Available Objective. To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA composite and evaluate its feasibility as a scaffold for cartilage tissue engineering. Methods. Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM. The cell cycle profile was analyzed by flow cytometry. Results. The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P>0.05. Conclusion. The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

  4. Structure, mechanical property and corrosion behaviors of (HA + β-TCP)/Mg–5Sn composite with interpenetrating networks

    Energy Technology Data Exchange (ETDEWEB)

    Wang, X., E-mail: wangxiang@hrbeu.edu.cn [Center for Biomedical Materials and Engineering, Key Laboratory of Superlight Material and Surface Technology, Ministry of Education, Harbin Engineering University, Harbin, 150001 (China); Li, J.T.; Xie, M.Y. [Center for Biomedical Materials and Engineering, Key Laboratory of Superlight Material and Surface Technology, Ministry of Education, Harbin Engineering University, Harbin, 150001 (China); Qu, L.J. [Key Lab of Biomedical Materials of University in Heilongjiang Province, Jiamusi University, Jiamusi 154007 (China); Zhang, P.; Li, X.L. [Center for Biomedical Materials and Engineering, Key Laboratory of Superlight Material and Surface Technology, Ministry of Education, Harbin Engineering University, Harbin, 150001 (China)

    2015-11-01

    In this paper, a novel (Hydroxyapatite + β-tricalcium phosphate)/Mg–5Sn ((HA + β-TCP)/Mg–5Sn) composite with interpenetrating networks was fabricated by infiltrating Mg–5Sn alloy into porous HA + β-TCP using suction casting technique. The structure, mechanical property and corrosion behaviors of the composite have been evaluated by means of scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical testing, electrochemical and immersion test. It is shown that the molten Mg–5Sn alloy has infiltrated not only into the pores but also into the struts of the HA + β-TCP scaffold to forming a compact composite. The microstructure observation also shows that the Mg alloy contacts to the HA + β-TCP closely, and no reaction layer can be found between Mg–5Sn alloy and scaffold. The ultimate compressive strength of the composite is as high as 176 MPa, which is about four fifths of the strength of the Mg–5Sn bulk alloy. The electrochemical and immersion tests indicate that the corrosion resistance of the composite is better than that of the Mg–5Sn bulk alloy. The corrosion products on the composite surface are mainly Mg(OH){sub 2}, Ca{sub 3}(PO{sub 4}){sub 2} and HA. Appropriate mechanical and corrosion properties of the (HA + β-TCP)/Mg–5Sn composite indicate its possibility for new bone tissue implant materials. - Highlights: • The novel (HA + β-TCP)/Mg–5Sn composite with interpenetrating networks was fabricated using suction casting technique. • The ultimate compressive strength of the composite was near with the natural bone. • And the corrosion resistance of the composite was better than that of the Mg–5Sn bulk alloy.

  5. Development of single-chain variable fragments (scFv) against influenza virus targeting hemagglutinin subunit 2 (HA2).

    Science.gov (United States)

    Li, Tai-Wei; Cheng, Shu-Fang; Tseng, Yen-Tzu; Yang, Yu-Chih; Liu, Wen-Chun; Wang, Sheng-Cyuan; Chou, Mei-Ju; Lin, Yu-Jen; Wang, Yueh; Hsiao, Pei-Wen; Wu, Suh-Chin; Chang, Ding-Kwo

    2016-01-01

    Influenza A viruses (IAV) are widespread in birds and domestic poultry, occasionally causing severe epidemics in humans and posing health threats. Hence, the need to develop a strategy for prophylaxis or therapy, such as a broadly neutralizing antibody against IAV, is urgent. In this study, single-chain variable fragment (scFv) phage display technology was used to select scFv fragments recognizing influenza envelope proteins. The Tomlinson I and J scFv phage display libraries were screened against the recombinant HA2 protein (rHA2) for three rounds. Only the third-round elution sample of the Tomlinson J library showed high binding affinity to rHA2, from which three clones (3JA18, 3JA62, and 3JA78) were chosen for preparative-scale production as soluble antibody by E. coli. The clone 3JA18 was selected for further tests due to its broad affinity for influenza H1N1, H3N2 and H5N1. Simulations of the scFv 3JA18-HA trimer complex revealed that the complementarity-determining region of the variable heavy chain (VH-CDR2) bound the stem region of HA. Neutralization assays using a peptide derived from VH-CDR2 also supported the simulation model. Both the selected antibody and its derived peptide were shown to suppress infection with H5N1 and H1N1 viruses, but not H3N2 viruses. The results also suggested that the scFvs selected from rHA2 could have neutralizing activity by interfering with the function of the HA stem region during virus entry into target cells.

  6. Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H

    Energy Technology Data Exchange (ETDEWEB)

    Yao, Guorui; Lam, Kwok-ho; Perry, Kay; Weisemann, Jasmin; Rummel, Andreas; Jin, Rongsheng (Cornell); (Dusseldorf); (UCI)

    2017-03-01

    Botulinum neurotoxins (BoNTs), which have been exploited as cosmetics and muscle-disorder treatment medicines for decades, are well known for their extreme neurotoxicity to humans. They pose a potential bioterrorism threat because they cause botulism, a flaccid muscular paralysis-associated disease that requires immediate antitoxin treatment and intensive care over a long period of time. In addition to the existing seven established BoNT serotypes (BoNT/A–G), a new mosaic toxin type termed BoNT/HA (aka type FA or H) was reported recently. Sequence analyses indicate that the receptor-binding domain (HC) of BoNT/HA is ~84% identical to that of BoNT/A1. However, BoNT/HA responds differently to some potent BoNT/A-neutralizing antibodies (e.g., CR2) that target the HC. Therefore, it raises a serious concern as to whether BoNT/HA poses a new threat to our biosecurity. In this study, we report the first high-resolution crystal structure of BoNT/HA-HC at 1.8 Å. Sequence and structure analyses reveal that BoNT/HA and BoNT/A1 are different regarding their binding to cell-surface receptors including both polysialoganglioside (PSG) and synaptic vesicle glycoprotein 2 (SV2). Furthermore, the new structure also provides explanations for the ~540-fold decreased affinity of antibody CR2 towards BoNT/HA compared to BoNT/A1. Taken together, these new findings advance our understanding of the structure and function of this newly identified toxin at the molecular level, and pave the way for the future development of more effective countermeasures

  7. Enantiomers of HA-966 (3-amino-1-hydroxypyrrolid-2-one) exhibit distinct central nervous system effects: (+)-HA-966 is a selective glycine/N-methyl-D-aspartate receptor antagonist, but (-)-HA-966 is a potent gamma-butyrolactone-like sedative

    Energy Technology Data Exchange (ETDEWEB)

    Singh, L.; Donald, A.E.; Foster, A.C.; Hutson, P.H.; Iversen, L.L.; Iversen, S.D.; Kemp, J.A.; Leeson, P.D.; Marshall, G.R.; Oles, R.J.; Priestley, T.; Thorn, L.; Tricklebank, M.D.; Vass, C.A.; Williams, B.J. (Merck Sharp and Dohme Research Labs., Essex (England))

    1990-01-01

    The antagonist effect of {+-}-3-amino-1-hydroxypyrrolid-2-one (HA-966) at the N-methyl-D-aspartate (NMDA) receptor occurs through a selective interaction with the glycine modulatory site within the receptor complex. When the enantiomers of {+-}-HA-966 were resolved, the (R)-(+)-enantiomer was found to be a selective glycine/NMDA receptor antagonist, a property that accounts for its anticonvulsant activity in vivo. In contrast, the (S)-(-)-enantiomer was only weakly active as an NMDA-receptor antagonist, but nevertheless it possessed a marked sedative and muscle relaxant action in vivo. In radioligand binding experiments, (+)-HA-966 inhibited strychnine-insensitive ({sup 3}H)glycine binding to rat cerebral cortex synaptic membranes with an IC{sub 50} of 12.5 {mu}M, whereas (-)-HA-966 had an IC{sub 50} value of 339 {mu}M. In mice, (+)-HA-966 antagonized sound and N-methyl-DL-aspartic acid (NMDLA)-induced seizures. The coadministration of D-serine dose-dependently antagonized the anticonvulsant effect of a submaximal dose of (+)-HA-966 against NMDLA-induced seizures. The sedative/ataxic effect of racemic HA-966 was mainly attributable to the (-)-enantiomer. It is suggested that, as in the case of the sedative {gamma}-butyrolactone, disruption of striatal dopaminergic mechanisms may be responsible for this action.

  8. The physiological response of two green calcifying algae from the Great Barrier Reef towards high dissolved inorganic and organic carbon (DIC and DOC availability.

    Directory of Open Access Journals (Sweden)

    Friedrich Wilhelm Meyer

    Full Text Available Increasing dissolved inorganic carbon (DIC concentrations associated with ocean acidification can affect marine calcifiers, but local factors, such as high dissolved organic carbon (DOC concentrations through sewage and algal blooms, may interact with this global factor. For calcifying green algae of the genus Halimeda, a key tropical carbonate producer that often occurs in coral reefs, no studies on these interactions have been reported. These data are however urgently needed to understand future carbonate production. Thus, we investigated the independent and combined effects of DIC (pCO2 402 μatm/ pHtot 8.0 and 996 μatm/ pHtot 7.7 and DOC (added as glucose in 0 and 294 μmol L-1 on growth, calcification and photosynthesis of H. macroloba and H. opuntia from the Great Barrier Reef in an incubation experiment over 16 days. High DIC concentrations significantly reduced dark calcification of H. opuntia by 130 % and led to net dissolution, but did not affect H. macroloba. High DOC concentrations significantly reduced daily oxygen production of H. opuntia and H. macroloba by 78 % and 43 %, respectively, and significantly reduced dark calcification of H. opuntia by 70%. Combined high DIC and DOC did not show any interactive effects for both algae, but revealed additive effects for H. opuntia where the combination of both factors reduced dark calcification by 162 % compared to controls. Such species-specific differences in treatment responses indicate H. opuntia is more susceptible to a combination of high DIC and DOC than H. macroloba. From an ecological perspective, results further suggest a reduction of primary production for Halimeda-dominated benthic reef communities under high DOC concentrations and additional decreases of carbonate accretion under elevated DIC concentrations, where H. opuntia dominates the benthic community. This may reduce biogenic carbonate sedimentation rates and hence the buffering capacity against further ocean

  9. Effect of phytase application during high gravity (HG) maize mashes preparation on the availability of starch and yield of the ethanol fermentation process.

    Science.gov (United States)

    Mikulski, D; Kłosowski, G; Rolbiecka, A

    2014-10-01

    Phytic acid present in raw materials used in distilling industry can form complexes with starch and divalent cations and thus limit their biological availability. The influence of the enzymatic hydrolysis of phytate complexes on starch availability during the alcoholic fermentation process using high gravity (HG) maize mashes was analyzed. Indicators of the alcoholic fermentation as well as the fermentation activity of Saccharomyces cerevisiae D-2 strain were statistically evaluated. Phytate hydrolysis improved the course of the alcoholic fermentation of HG maize mashes. The final ethanol concentration in the media supplemented with phytase applied either before or after the starch hydrolysis increased by 1.0 and 0.6 % v/v, respectively, as compared to the control experiments. This increase was correlated with an elevated fermentation yield that was higher by 5.5 and 2.0 L EtOH/100 kg of starch, respectively. Phytate hydrolysis resulted also in a statistically significant increase in the initial concentration of fermenting sugars by 14.9 mg/mL of mash, on average, which was a consequence of a better availability of starch for enzymatic hydrolysis. The application of phytase increased the attenuation of HG media fermentation thus improving the economical aspect of the ethanol fermentation process.

  10. Downgrading recent estimates of land available for biofuel production.

    Science.gov (United States)

    Fritz, Steffen; See, Linda; van der Velde, Marijn; Nalepa, Rachel A; Perger, Christoph; Schill, Christian; McCallum, Ian; Schepaschenko, Dmitry; Kraxner, Florian; Cai, Ximing; Zhang, Xiao; Ortner, Simone; Hazarika, Rubul; Cipriani, Anna; Di Bella, Carlos; Rabia, Ahmed H; Garcia, Alfredo; Vakolyuk, Mar'yana; Singha, Kuleswar; Beget, Maria E; Erasmi, Stefan; Albrecht, Franziska; Shaw, Brian; Obersteiner, Michael

    2013-02-05

    Recent estimates of additional land available for bioenergy production range from 320 to 1411 million ha. These estimates were generated from four scenarios regarding the types of land suitable for bioenergy production using coarse-resolution inputs of soil productivity, slope, climate, and land cover. In this paper, these maps of land availability were assessed using high-resolution satellite imagery. Samples from these maps were selected and crowdsourcing of Google Earth images was used to determine the type of land cover and the degree of human impact. Based on this sample, a set of rules was formulated to downward adjust the original estimates for each of the four scenarios that were previously used to generate the maps of land availability for bioenergy production. The adjusted land availability estimates range from 56 to 1035 million ha depending upon the scenario and the ruleset used when the sample is corrected for bias. Large forest areas not intended for biofuel production purposes were present in all scenarios. However, these numbers should not be considered as definitive estimates but should be used to highlight the uncertainty in attempting to quantify land availability for biofuel production when using coarse-resolution inputs with implications for further policy development.

  11. Biochar from sugarcane filtercake reduces soil CO2 emissions relative to raw residue and improves water retention and nutrient availability in a highly-weathered tropical soil.

    Directory of Open Access Journals (Sweden)

    Angela Joy Eykelbosh

    Full Text Available In Brazil, the degradation of nutrient-poor Ferralsols limits productivity and drives agricultural expansion into pristine areas. However, returning agricultural residues to the soil in a stabilized form may offer opportunities for maintaining or improving soil quality, even under conditions that typically promote carbon loss. We examined the use of biochar made from filtercake (a byproduct of sugarcane processing on the physicochemical properties of a cultivated tropical soil. Filtercake was pyrolyzed at 575°C for 3 h yielding a biochar with increased surface area and porosity compared to the raw filtercake. Filtercake biochar was primarily composed of aromatic carbon, with some residual cellulose and hemicellulose. In a three-week laboratory incubation, CO2 effluxes from a highly weathered Ferralsol soil amended with 5% biochar (dry weight, d.w. were roughly four-fold higher than the soil-only control, but 23-fold lower than CO2 effluxes from soil amended with 5% (d.w. raw filtercake. We also applied vinasse, a carbon-rich liquid waste from bioethanol production typically utilized as a fertilizer on sugarcane soils, to filtercake- and biochar-amended soils. Total CO2 efflux from the biochar-amended soil in response to vinasse application was only 5% of the efflux when vinasse was applied to soil amended with raw filtercake. Furthermore, mixtures of 5 or 10% biochar (d.w. in this highly weathered tropical soil significantly increased water retention within the plant-available range and also improved nutrient availability. Accordingly, application of sugarcane filtercake as biochar, with or without vinasse application, may better satisfy soil management objectives than filtercake applied to soils in its raw form, and may help to build soil carbon stocks in sugarcane-cultivating regions.

  12. Niche shifts and energetic condition of songbirds in response to phenology of food-resource availability in a high-elevation sagebrush ecosystem

    Science.gov (United States)

    Cutting, Kyle A.; Anderson, Michelle L.; Beever, Erik; Schroff, Sean; Korb, Nathan; Klaphake, Eric; McWilliams, Scott R.

    2016-01-01

    Seasonal fluctuations in food availability can affect diets of consumers, which in turn may influence the physiological state of individuals and shape intra- and inter-specific patterns of resource use. High-elevation ecosystems often exhibit a pronounced seasonal “pulse” in productivity, although few studies document how resource use and energetic condition by avian consumers change in relation to food-resource availability in these ecosystems. We tested the hypothesis that seasonal increases (pulses) in food resources in high-elevation sagebrush ecosystems result in 2 changes after the pulse, relative to the before-pulse period: (1) reduced diet breadth of, and overlap between, 2 sympatric sparrow species; and (2) enhanced energetic condition in both species. We tracked breeding-season diets using stable isotopes and energetic condition using plasma metabolites of Brewer's Sparrows (Spizella breweri), Vesper Sparrows (Pooecetes gramineus), and their food resources during 2011, and of only Brewer's Sparrows and their food resources during 2013. We quantify diet breadth and overlap between both species, along with coincident physiological consequences of temporal changes in resource use. After invertebrate biomass increased following periods of rainfall in 2011, dietary breadth decreased by 35% in Brewer's Sparrows and by 48% in Vesper Sparrows, while dietary overlap decreased by 88%. Energetic condition of both species increased when dietary overlap was lower and diet breadth decreased, after the rapid rise of food-resource availability. However, energetic condition of Brewer's Sparrows remained constant in 2013, a year with low precipitation and lack of a strong pulse in food resources, even though the species' dietary breadth again decreased that year. Our results indicate that diet breadth and overlap in these sparrow species inhabiting sagebrush ecosystems generally varied as predicted in relation to intra- and interannual changes in food resources, and

  13. Degradation behavior and compatibility of micro, nanoHA/chitosan scaffolds with interconnected spherical macropores.

    Science.gov (United States)

    Ruixin, Li; Cheng, Xu; Yingjie, Liu; Hao, Li; Caihong, Shi; Weihua, Su; Weining, An; Yinghai, Yuan; Xiaoli, Qin; Yunqiang, Xu; Xizheng, Zhang; Hui, Li

    2017-03-30

    Hydroxyapatite/Chitosan (HA/CS) composite have significant application in biomedical especially for bone replacement. Inorganic particle shape and size of composite affect the scaffold mechanical property, biological property, and degradation. The aim of this study was to fabricate HA/CS scaffold with good pore connectivity and analyze their biological, degradation properties. Microhydroxyapatite/chitosan(mHA/CS) and nanohydroxyapatite/chitosan (nHA/CS) composite scaffolds with interconnected spherical pore architectures were fabricated. Composite scaffolds structure parameters were analyzed using micro CT. Cell proliferation and morphology were tested and compared between two scaffolds using mouse osteoblastic cell line MC3T3-E1. To research the composite degradation in lysozyme PBS solution, degradation rate and reducing sugar content were tested, and scaffolds morphology were observed by SEM. The results showed that microHA and nanoHA were fabricated by being calcined and synthesis methods, and their infrared spectra are very similar. EDAX composition analysis demonstrated that both of microHA and nanoHA were calcium deficiency HA. Micro-CT results demonstrated the scaffolds had interconnected spherical pores, and the structure parameters were similar. Cell viabilities were significant increased with cultured time, but there were no significant difference between microHA/CS and nanoHA/CS scaffolds. Scaffold structure was gradually destroyed and inorganic composition HA particles are more prominent with degradation time.

  14. Preparation and application of hydroxyapatite(HA) nanoparticles/NR2B-siRNA complex

    Institute of Scientific and Technical Information of China (English)

    YANG Hui; HUANG Dong; ZHU Shai-hong; YAN Xue-bin; GU Yong-hong; YAN Hui; WU Li-xiang

    2008-01-01

    Hydroxyapatite(HA) nanoparticles were prepared by coprecipitation-hydrothermal synthesis and their exosyndrome was estimated via transmission electron microscopy. Agarose gel electrophoresis and ultraviolet spectrophotometry were used to evaluate the ability of HA to bind NR2B-siRNA at different pH values and at different HA-NR2B-siRNA ratios. And the stability of the complex in saline was also evaluated. The effect of HA/NR2B-siRNA complex on chronic inflammatory pain was evaluated in vivo in mice. Transmission electron microscopy(TEM) reveals that HA nanoparticles are thin strips or short rod in shape and the one-dimensional particle size of HA nanoparticles is 40-50 nm. Under the acid or neutral condition, the Zeta potential of HA is positive; nanoparticles can completely bind NR2B-siRNA when the HA:NR2B-siRNA ratio is at or larger than 35-1; while under the alkaline condition, the affinity of HA to NR2B-siRNA is rather weak. HA/NR2B-siRNA complex is not dissociated when being resuspended in saline. The nociception of the tonic phase induced by formalin is significantly reduced in the HA/NR2B-siRNA treated mice as compared with the controls. Therefore, HA may be a new siRNA nano-vector material.

  15. Qimḥi’s Sefer ha-Shorashim: A Didactic Tool

    Directory of Open Access Journals (Sweden)

    Judith Kogel

    2017-01-01

    Full Text Available Less than forty years after Judah ibn Tibbon translated Ibn Janāḥ’s Kitāb al-Uṣūl into Hebrew, David Qimḥi availed himself to write a new dictionary of Hebrew roots, known as Sefer ha-Shorashim. This book achieved great success and consequently overshadowed the work of his predecessor which nevertheless served as a model to Qimḥi. A preliminary research based on the roots starting by the letter ṭet and sameḵ seems to constitute a representative sample for the history of the text. First of all, it allows to better grasp Qimḥi’s motivation for writing a new dictionary. Further, the analysis of this relatively small corpus gives us clues to understand Qimḥi’s lexicological approach to the Hebrew root and his strategy in the organization of each entry.

  16. Long-term high-temperature and pH stability assessment of modern commercially available stationary phases by using retention factor analysis.

    Science.gov (United States)

    Haun, Jakob; Oeste, Katja; Teutenberg, Thorsten; Schmidt, Torsten C

    2012-11-09

    High-temperature liquid chromatography provides several advantages concerning HPLC method development and is needed to realize special hyphenation techniques. However, hardware limitations and stationary phase degradation can prevent the successful application of this technique. Therefore, column stability is of major importance. The presented study contains results of long-term high-temperature stability tests for eight modern commercially available HPLC stationary phases. Six of them were silica-based reversed-phase C18 columns tested according to an earlier reported procedure (Teutenberg, 2009 [12]). On the basis of the extended data set that covers a wide range of column technologies, a comparative approach using retention factor analysis was evaluated in order to categorize the columns according to their long-term high-temperature stability at 150 °C and changing pH value. This approach offers a more objective alternative to conventional listings in which temperature maxima are compared for columns tested under different conditions. Additionally, customized tests are presented for one promising polymer-based C18 stationary phase and an amide column for hydrophilic interaction liquid chromatography.

  17. Available phosphorus levels in diets supplemented with phytase for male broilers aged 22 to 42 days kept in a high-temperature environment

    Directory of Open Access Journals (Sweden)

    Tarciso Tizziani

    2016-02-01

    Full Text Available ABSTRACT This study was conducted to evaluate the effect of reduction of the available phosphorus (avP in diets supplemented with 500 FTU/kg phytase on performance, carcass characteristics, and bone mineralization of broilers aged 22 to 42 days kept in a high-temperature environment. A total of 336 Cobb broilers with an average initial weight of 0.883±0.005 kg were distributed in a completely randomized design with six treatments - a positive control (0.354 and 0.309% avP without addition of bacterial phytase for the phases of 22 to 33 and 34 to 42 days, respectively, and another five diets with inclusion of phytase (500 FTU and reduction of the level of avP (0.354, 0.294, 0.233, 0.173, and 0.112%; and 0.309, 0.258, 0.207, 0.156, and 0.106% for the phases of 22 to 33 and 34 to 42 days, respectively - eight replicates, and seven birds per cage. The experimental diets were formulated to meet all nutritional requirements, except for avP and calcium. Birds were kept in climatic chambers at a temperature of 32.2±0.4 °C and air humidity of 65.3±5.9%. Phytase acted by making the phytate P available in diets with reduction in the levels of avP, keeping feed intake, weight gain, feed:gain, and carcass characteristics unchanged. Treatments affected ash and calcium deposition and the Ca:P ratio in the bone; the group fed the diets with 0.112 and 0.106%, from 22 to 33 and 34 to 42 days of age, respectively, obtained the lowest values, although the phosphorus deposition in the bone was not affected. Diets supplemented with 500 FTU of phytase, with available phosphorus reduced to 0.173 and 0.156%, and a fixed Ca:avP ratio of 2.1:1, meet the requirements of broilers aged 22 to 33 and 34 to 42 days, respectively, reared in a high-temperature environment.

  18. 超音速氧焰喷涂HA/BG涂层的制备及表征%Research on Properties of HA/BG Coatings Prepared by HVOF Spraying

    Institute of Scientific and Technical Information of China (English)

    鲍益富; 翁杰; 冯波; 段可

    2009-01-01

    Hydroxyapatite (HA) coatings with bioactive glass (BG) was prepared on Ti6A14V matrix by high velocity oxygen-fuel spray. The phase morphology and bioactivity of the coatings were studied. The X-ray diffraction shows that the coatings are mainly crystalline HA and exhibit no thermal decomposition byproducts. The addition of BG does not affect the coating phases. The SEM observation shows that the HA particles are partially melted, with melting fraction of 13%, and spherical BG particles are incorporated on the HA coating. The BG content of the surface coating is lower than that in input powder. After soaking in simulated body fluid for 7 days, only the coating with 20% BG forms a bone-like apatite, which indicates that the addition of BG increases the bioactivity of the coating.%利用超音速氧焰喷涂(HVOF)在Ti6A14V基体上制备了羟基磷灰石(HA)/生物玻璃(BG)涂层,考察了涂层的相组成、表面形貌及生物活性.XRD显示:涂层的结晶相为HA,未检测到HA分解产物,添加生物玻璃不影响涂层的相组成;SEM结果表明:HA颗粒熔化较少,计算熔化比例为13%,BG颗粒以球形方式镶嵌在涂层表面,含量小于粉末中的比例:涂层浸泡在模拟体液中7天后发现:添加20%BG的涂层表面有类骨类磷灰石涂层生成,说明添加BG可以提高涂层的生物活性.

  19. Áreas metropolitanas:¿qué ha cambiado?: La experiencia de la Caracas Metropolitana

    Directory of Open Access Journals (Sweden)

    Sonia Barrios

    2001-05-01

    Full Text Available La nueva economía de la información y del conocimiento ha tenido tres decisivas implicaciones de orden territorial. Primero, ha acelerado el proceso de aglomeración urbana a escala planetaria. Segundo, ha provocado fuertes alteraciones en las redes urbanas de ámbito mundial, regional y nacional. Y tercero, ha impulsado la reestructuración interna de las áreas metropolitanas que se habían conformado durante la era industrial. Todo parece anticipar, por lo tanto, polarizaciones y fragmentaciones territoriales todavía más acentuadas que las conocidas en un pasado cercano. Tomando como referencia el caso de Caracas, se trata de averiguar en qué medida el desarrollo reciente de esta metrópoli sigue las grandes tendencias territoriales antes anotadas; y qué esfuerzos se están haciendo para ajustar sus instancias locales a las nuevas realidades urbanas. Como un recurso adicional, en distintos momentos se establecen comparaciones con Barcelona (España, considerada un ejemplo emblemático de las metrópolis de la era de la informaciónThe developments of new informational and knowledge-based economies have had three decisive implications at the territorial level. First, it has accelerated the urban agglomeration processes worldwide. Second, it has brought about substantial alterations in the existing global, regional and national urban networks. Finally, it has triggered the internal restructuring of former industrial-based metropolitan areas. All this seems to anticipate that territorial polarization and fragmentation will reach higher levels than those we have observed in the recent past. Taking the case of Caracas as a reference point, the intention is to find out to what extent the recent development of this metropolis follows the main territorial trends mentioned above. Interest is also placed in finding what steps are being taken in order to adjust its local levels of government to the new urban realities. In addition, comparisons are

  20. Titanium Dioxide Nanoparticle Penetration into the Skin and Effects on HaCaT Cells.

    Science.gov (United States)

    Crosera, Matteo; Prodi, Andrea; Mauro, Marcella; Pelin, Marco; Florio, Chiara; Bellomo, Francesca; Adami, Gianpiero; Apostoli, Pietro; De Palma, Giuseppe; Bovenzi, Massimo; Campanini, Marco; Filon, Francesca Larese

    2015-08-07

    Titanium dioxide nanoparticles (TiO2NPs) suspensions (concentration 1.0 g/L) in synthetic sweat solution were applied on Franz cells for 24 h using intact and needle-abraded human skin. Titanium content into skin and receiving phases was determined. Cytotoxicity (MTT, AlamarBlue(®) and propidium iodide, PI, uptake assays) was evaluated on HaCat keratinocytes after 24 h, 48 h, and seven days of exposure. After 24 h of exposure, no titanium was detectable in receiving solutions for both intact and damaged skin. Titanium was found in the epidermal layer after 24 h of exposure (0.47 ± 0.33 μg/cm(2)) while in the dermal layer, the concentration was below the limit of detection. Damaged skin, in its whole, has shown a similar concentration (0.53 ± 0.26 μg/cm(2)). Cytotoxicity studies on HaCaT cells demonstrated that TiO2NPs induced cytotoxic effects only at very high concentrations, reducing cell viability after seven days of exposure with EC50s of 8.8 × 10(-4) M (MTT assay), 3.8 × 10(-5) M (AlamarBlue(®) assay), and 7.6 × 10(-4) M (PI uptake, index of a necrotic cell death). Our study demonstrated that TiO2NPs cannot permeate intact and damaged skin and can be found only in the stratum corneum and epidermis. Moreover, the low cytotoxic effect observed on human HaCaT keratinocytes suggests that these nano-compounds have a potential toxic effect at the skin level only after long-term exposure.

  1. Efficient CD44-targeted magnetic resonance imaging (MRI) of breast cancer cells using hyaluronic acid (HA)-modified MnFe2O4 nanocrystals

    Science.gov (United States)

    Lee, Taeksu; Lim, Eun-Kyung; Lee, Jaemin; Kang, Byunghoon; Choi, Jihye; Park, Hyo Seon; Suh, Jin-Suck; Huh, Yong-Min; Haam, Seungjoo

    2013-04-01

    Targeted molecular imaging with hyaluronic acid (HA) has been highlighted in the diagnosis and treatment of CD44-overexpressing cancer. CD44, a receptor for HA, is closely related to the growth of cancer including proliferation, metastasis, invasion, and angiogenesis. For the efficient detection of CD44, we fabricated a few kinds of HA-modified MnFe2O4 nanocrystals (MNCs) to serve as specific magnetic resonance (MR) contrast agents (HA-MRCAs) and compared physicochemical properties, biocompatibility, and the CD44 targeting efficiency. Hydrophobic MNCs were efficiently phase-transferred using aminated polysorbate 80 (P80) synthesized by introducing spermine molecules on the hydroxyl groups of P80. Subsequently, a few kinds of HA-MRCAs were fabricated, conjugating different ratios of HA on the equal amount of phase-transferred MNCs. The optimized conjugation ratio of HA against magnetic content was identified to exhibit not only effective CD44 finding ability but also high cell viability through in vitro experiments. The results of this study demonstrate that the suggested HA-MRCA shows strong potential to be used for accurate tumor diagnosis.

  2. DEMENCIA FRONTOTEMPORAL, CÓMO HA RESURGIDO SU DIAGNÓSTICO

    Directory of Open Access Journals (Sweden)

    Patricia Lillo Z, MD, PhD

    2016-05-01

    Full Text Available Desde la descripción inicial efectuada por Pick hace más de un siglo atrás, el interés por las demencias frontotemporales ha tenido un gran crecimiento. Gracias a los avances de las neuroimágenes y el desarrollo de biomarcadores, el progreso en este campo ha generado nuevos conocimientos en torno a la categorización clínica, correlatos neuronales de las funciones cerebrales superiores y mecanismos de neurodegeneración. Paradójicamente, estos avances han traído consigo cierta controversia y desafíos, los cuales han motivado la creación de nuevos criterios diagnósticos. Esta breve revisión muestra la evolución histórica de estas condiciones y describe los criterios diagnósticos actuales para la variante conductual y las afasias progresivas primarias, que son las dos formas clínicas de las demencias frontotemporales.

  3. LA BIOÉTICA HA MUERTO. ¡VIVA LA ÉTICA MÉDICA!

    Directory of Open Access Journals (Sweden)

    JOSÉ MARÍA BARRIO MAESTRE

    2015-01-01

    Full Text Available El objeto de este artículo es mostrar la crisis paradigmática que vive la bioética académica. Desde que una parte importante del gremio de los bioeticistas comenzó a relativizar la prohibición ética de dar muerte a un ser humano inocente, de una forma u otra comenzó a aliarse con la industria de la muerte: el negocio del aborto provocado y, después, de la eutanasia. La tesis de este trabajo es que al cruzar ese Rubicón la bioética se ha corrompido, y ha perdido su conexión con el discurso ético, político y jurídico. Sólo cabe esperar que resurja de sus cenizas si recupera el «tabú» de la sacralidad de la vida humana, algo para lo que la Ética Médica podría suministrar una ayuda inestimable, pues aún se conserva ahí la referencia de que «un médico no debe matar», si bien en forma excesivamente «discreta», y algo «avergonzada». De todos modos, los médicos con conciencia saben más de ética que la mayor parte de los bioeticistas.

  4. Formulation Changes Affect Material Properties and Cell Behavior in HA-Based Hydrogels

    Directory of Open Access Journals (Sweden)

    Thomas Lawyer

    2012-01-01

    Full Text Available To develop and optimize new scaffold materials for tissue engineering applications, it is important to understand how changes to the scaffold affect the cells that will interact with that scaffold. In this study, we used a hyaluronic acid- (HA- based hydrogel as a synthetic extracellular matrix, containing modified HA (CMHA-S, modified gelatin (Gtn-S, and a crosslinker (PEGda. By varying the concentrations of these components, we were able to change the gelation time, enzymatic degradation, and compressive modulus of the hydrogel. These changes also affected fibroblast spreading within the hydrogels and differentially affected the proliferation and metabolic activity of fibroblasts and mesenchymal stem cells (MSCs. In particular, PEGda concentration had the greatest influence on gelation time, compressive modulus, and cell spreading. MSCs appeared to require a longer period of adjustment to the new microenvironment of the hydrogels than fibroblasts. Fibroblasts were able to proliferate in all formulations over the course of two weeks, but MSCs did not. Metabolic activity changed for each cell type during the two weeks depending on the formulation. These results highlight the importance of determining the effect of matrix composition changes on a particular cell type of interest in order to optimize the formulation for a given application.

  5. Cohort Profile: The Montreal Neighbourhood Networks and Healthy Aging (MoNNET-HA) study.

    Science.gov (United States)

    Moore, Spencer; Buckeridge, David L; Dubé, Laurette

    2016-02-01

    The Montreal Neighbourhood Networks and Healthy Aging study was established: (i) to assess the added value in using formal network methods and instruments to measure social capital and its relationship to health; (ii) to determine whether older adults are more vulnerable to the effects of network and neighbourhood environments; and (iii) to examine longitudinally the relationship between social capital and health among adults in Montreal, Canada. The MoNNET-HA cohort consists of men and women aged 25 years and older, residing in the Montreal Metropolitan Area (MMA). Participants were recruited using a random stratified cluster sampling design with oversampling of adults older than 65 years. Initial MoNNET-HA study participants (n = 2707) were recruited for telephone interviews in the summer of 2008. Since 2008, participants were interviewed in the autumn of 2010 and the winter of 2013/2014. Data currently fall into five categories: (i) social network and social capital; (ii) psychosocial and psychological; (ii) socio-demographic and socioeconomic; (iv) health behaviours and conditions; and (v) neighbourhood environmental characteristics. Healthcare utilization data will be available for a subsample of participants. Upon funding, future work will measure anthropometric and metabolic health directly. Based on agreements with participants, external researchers should request access to data via collaborations with the study group.

  6. School and District Wellness Councils and Availability of Low-Nutrient, Energy-Dense Vending Fare in Minnesota Middle and High Schools

    Science.gov (United States)

    Kubik, Martha Y.; Lytle, Leslie A.; Farbakhsh, Kian

    2011-01-01

    The Child Nutrition and WIC Reauthorization Act of 2004 required school districts participating in the federal school meals program to establish by the start of the 2006–2007 school year policies that included nutrition guidelines for all foods sold on school campus during the school day and policy development involving key stakeholders. For many schools, policy development was done by wellness councils. This study examined the association between having a wellness council and availability of low-nutrient, energy-dense foods/beverages in school vending machines following enactment of the federal legislation. In 2006–2007, Minnesota middle (n=35) and high (n=54) school principals reported whether their school and district had a wellness council. Trained research staff observed foods/beverages in vending machines accessible to students. Low-nutrient, energy-dense foods/beverages (snacks >3 g fat or >200 calories/serving, and soda, fruit/sport drinks and reduced-fat/whole milk) were grouped into seven categories (eg, high-fat baked goods) and a food score was calculated. Higher scores indicated more low-nutrient, energy-dense vending fare. Multivariate linear regression, adjusted for school characteristics, was used to examine associations between scores and a three-category council variable (district-only; district and school; no council). Among schools, 53% had district-only councils, 38% district and school councils, and 9% had no council. Schools with both a district and school council had a significantly lower mean food score than schools without councils (P=0.03). The potential of wellness councils to impact availability of low-nutrient, energy-dense vending fare is promising. There may be an added benefit to having both a school and district council. PMID:21185978

  7. Biochar from Sugarcane Filtercake Reduces Soil CO2 Emissions Relative to Raw Residue and Improves Water Retention and Nutrient Availability in a Highly-Weathered Tropical Soil

    Science.gov (United States)

    Eykelbosh, Angela Joy; Johnson, Mark S.; Santos de Queiroz, Edmar; Dalmagro, Higo José; Guimarães Couto, Eduardo

    2014-01-01

    In Brazil, the degradation of nutrient-poor Ferralsols limits productivity and drives agricultural expansion into pristine areas. However, returning agricultural residues to the soil in a stabilized form may offer opportunities for maintaining or improving soil quality, even under conditions that typically promote carbon loss. We examined the use of biochar made from filtercake (a byproduct of sugarcane processing) on the physicochemical properties of a cultivated tropical soil. Filtercake was pyrolyzed at 575°C for 3 h yielding a biochar with increased surface area and porosity compared to the raw filtercake. Filtercake biochar was primarily composed of aromatic carbon, with some residual cellulose and hemicellulose. In a three-week laboratory incubation, CO2 effluxes from a highly weathered Ferralsol soil amended with 5% biochar (dry weight, d.w.) were roughly four-fold higher than the soil-only control, but 23-fold lower than CO2 effluxes from soil amended with 5% (d.w.) raw filtercake. We also applied vinasse, a carbon-rich liquid waste from bioethanol production typically utilized as a fertilizer on sugarcane soils, to filtercake- and biochar-amended soils. Total CO2 efflux from the biochar-amended soil in response to vinasse application was only 5% of the efflux when vinasse was applied to soil amended with raw filtercake. Furthermore, mixtures of 5 or 10% biochar (d.w.) in this highly weathered tropical soil significantly increased water retention within the plant-available range and also improved nutrient availability. Accordingly, application of sugarcane filtercake as biochar, with or without vinasse application, may better satisfy soil management objectives than filtercake applied to soils in its raw form, and may help to build soil carbon stocks in sugarcane-cultivating regions. PMID:24897522

  8. School and district wellness councils and availability of low-nutrient, energy-dense vending fare in Minnesota middle and high schools.

    Science.gov (United States)

    Kubik, Martha Y; Lytle, Leslie A; Farbakhsh, Kian

    2011-01-01

    The Child Nutrition and WIC Reauthorization Act of 2004 required school districts participating in the federal school meals program to establish by the start of the 2006-2007 school year policies that included nutrition guidelines for all foods sold on school campus during the school day and policy development involving key stakeholders. For many schools, policy development was done by wellness councils. This study examined the association between having a wellness council and availability of low-nutrient, energy-dense foods/beverages in school vending machines following enactment of the federal legislation. In 2006-2007, Minnesota middle (n=35) and high (n=54) school principals reported whether their school and district had a wellness council. Trained research staff observed foods/beverages in vending machines accessible to students. Low-nutrient, energy-dense foods/beverages (snacks >3 g fat or >200 calories/serving, and soda, fruit/sport drinks and reduced-fat/whole milk) were grouped into seven categories (eg, high-fat baked goods) and a food score was calculated. Higher scores indicated more low-nutrient, energy-dense vending fare. Multivariate linear regression, adjusted for school characteristics, was used to examine associations between scores and a three-category council variable (district-only; district and school; no council). Among schools, 53% had district-only councils, 38% district and school councils, and 9% had no council. Schools with both a district and school council had a significantly lower mean food score than schools without councils (P=0.03). The potential of wellness councils to impact availability of low-nutrient, energy-dense vending fare is promising. There may be an added benefit to having both a school and district council.

  9. 向日葵病程相关蛋白 HaPR1基因的克隆与功能%Cloning and Function Analysis of Pathogenesis Related Protein Gene HaPR1 from Sunflower (Helianthus annuus)

    Institute of Scientific and Technical Information of China (English)

    马立功; 张匀华; 孟庆林; 石凤梅; 刘佳; 李易初; 王志英

    2015-01-01

    Pathogenesis-related proteins are commonly used as markers of plant defense responses. The full-length cDNA of pathogenesis-related protein 1 (PR1) named HaPR1 in Helianthus annuus was cloned based on the transcriptome of H. annuus induced by Sclerotinia sclerotiorum, and its expression model and function were analyzed in this study. Sequence analysis showed that the cDNA of HaPR1 (GenBank accession No. KR071874) contained a 489 bp ORF encoding a protein of 162 amino acids residues with the molecular mass of 17.52 kD and theoretical pI of 8.19, HaPR1 possessed six conserved cysteine and four con-served allergen V5/Tpx-1 related domain. The HaPR1 was highly homologous with PR1 in other species. Real-time PCR analysis showed that expression level of HaPR1 was the highest in leaf, and was significantly induced by drought, salt stress, oxalic acid, S. sclerotiorum and its metabolites. Then the HaPR1 gene was transformed into tobacco by Agrobacterium tumefaciens to further verify its function. The results showed that the expression of HaPR1 improved the resistance of transgenic lines, and significantly increased SOD, POD, and CAT activities and reduced the content of MDA. It suggested that HaPR1 has a function of resistance to S. sclerotiorum.%病程相关蛋白(pathogenesis-related proteins)经常被用作植物抗病反应的分子标记。本文在核盘菌诱导向日葵转录组文库的基础上克隆1个病程相关蛋白1基因 HaPR1的 cDNA 全长序列,并进行了表达模式和功能分析。结果表明,该基因 cDNA 全长开放阅读框为489 bp,编码162个氨基酸,分子量为17.52 kD,等电点为8.19,具有6个保守半胱氨酸,4个保守的 allergen V5/Tpx-1结构域, GenBank 登录号为 KR071874。经比较 HaPR1与多种物种 PR1高度同源。实时荧光定量 PCR 检测结果表明, HaPR1相对表达量在向日葵叶中最高,根中其次,茎中最低。干旱、盐、草酸、核盘菌及其代谢物均可显著诱导其表达。利用农

  10. How Integrated Management Strategies Promote Protein Quality of Cotton Embryos: High Levels of Soil Available N, N Assimilation and Protein Accumulation Rate.

    Science.gov (United States)

    Yang, HongKun; Meng, YaLi; Chen, BingLin; Zhang, XingYue; Wang, YouHua; Zhao, WenQing; Zhou, ZhiGuo

    2016-01-01

    Cottonseed is widely used as a source of ruminant feed and for industrial purposes. Therefore, there is a tremendous need to improve the nutritional value of cotton embryos. In this study, a conventional management (CM) and two integrated cotton management strategies (IMS1, IMS2) were performed at two soil fertility levels to study the relationships among soil N, N assimilation, embryonic protein accumulation and protein quality. The levels of proteins, essential amino acids, and semi-essential amino acids, especially those of glutamate, lysine, and methionine, were higher in IMS1 and IMS2 embryos than in CM embryos. These changes were significantly positively correlated with the soil-available N content, glutamine synthetase activity and peak value of protein accumulation rate and were negatively correlated with the free amino acid level. These results illustrated that integrated management strategies, especially the rates and timing of N application, raise the level of soil available N, which is beneficial for N assimilation in developing cotton embryos. The protein content was limited by the rate of protein accumulation rather than by the free amino acid content. The combination of target yield fertilization, a growth-driven N application schedule, a high plant density and the seedling raising with bio-organic fertilizer can substantially improve protein quality in cotton embryos, especially at a soil with low soil organic matter and total nitrogen.

  11. High altitude may alter oxygen availability and renal metabolism in diabetics as measured by hyperpolarized [1-1C]pyruvate magnetic resonance imaging

    DEFF Research Database (Denmark)

    Laustsen, Christoffer; Lycke, Sara; Palm, Fredrik;

    2014-01-01

    The kidneys account for about 10% of the whole body oxygen consumption, whereas only 0.5% of the total body mass. It is known that intrarenal hypoxia is present in several diseases associated with development of kidney disease, including diabetes, and when renal blood flow is unaffected. The impo...... of nephropathy in patients with diabetes at high altitudes may originate from the increased sensitivity toward inspired oxygen. This increased lactate production shifts the metabolic routs toward hypoxic pathways.......The kidneys account for about 10% of the whole body oxygen consumption, whereas only 0.5% of the total body mass. It is known that intrarenal hypoxia is present in several diseases associated with development of kidney disease, including diabetes, and when renal blood flow is unaffected....... The importance of deranged oxygen metabolism is further supported by deterioration of kidney function in patients with diabetes living at high altitude. Thus, we argue that reduced oxygen availability alters renal energy metabolism. Here, we introduce a novel magnetic resonance imaging (MRI) approach to monitor...

  12. Sediment availability on burned hillslopes

    Science.gov (United States)

    Nyman, Petter; Sheridan, Gary J.; Moody, John A.; Smith, Hugh G.; Noske, Philip J.; Lane, Patrick N. J.

    2013-12-01

    describes the inherent resistance of soil to erosion. Hillslope erosion models typically consider erodibility to be constant with depth. This may not be the case after wildfire because erodibility is partly determined by the availability of noncohesive soil and ash at the surface. This study quantifies erodibility of burned soils using methods that explicitly capture variations in soil properties with depth. Flume experiments on intact cores from three sites in western United States showed that erodibility of fire-affected soil was highest at the soil surface and declined exponentially within the top 20 mm of the soil profile, with root density and soil depth accounting for 62% of the variation. Variation in erodibility with depth resulted in transient sediment flux during erosion experiments on bounded field plots. Material that contributed to transient flux was conceptualized as a layer of noncohesive material of variable depth (dnc). This depth was related to shear strength measurements and sampled spatially to obtain the probability distribution of noncohesive material as a function of depth below the surface. After wildfire in southeast Australia, the initial dnc ranged from 7.5 to 9.1 mm, which equated to 97-117 Mg ha-1 of noncohesive material. The depth decreased exponentially with time since wildfire to 0.4 mm (or < 5 Mg ha-1) after 3 years of recovery. The results are organized into a framework for modeling fire effects on erodibility as a function of the production and depletion of the noncohesive layer overlying a cohesive layer.

  13. Immunopotentiation of Different Adjuvants on Humoral and Cellular Immune Responses Induced by HA1-2 Subunit Vaccines of H7N9 Influenza in Mice.

    Directory of Open Access Journals (Sweden)

    Li Song

    Full Text Available In spring 2013, human infections with a novel avian influenza A (H7N9 virus were reported in China. The number of cases has increased with over 200 mortalities reported to date. However, there is currently no vaccine available for the H7 subtype of influenza A virus. Virus-specific cellular immune responses play a critical role in virus clearance during influenza infection. In this study, we undertook a side-by-side evaluation of two different adjuvants, Salmonella typhimurium flagellin (fliC and polyethyleneimine (PEI, through intraperitoneal administration to assess their effects on the immunogenicity of the recombinant HA1-2 subunit vaccine of H7N9 influenza. The fusion protein HA1-2-fliC and HA1-2 combined with PEI could induce significantly higher HA1-2-specific IgG and hemagglutination inhibition titers than HA1-2 alone at 12 days post-boost, with superior HA1-2 specific IgG titers in the HA1-2-fliC group compared with the PEI adjuvanted group. The PEI adjuvanted vaccine induced higher IgG1/IgG2a ratio and significantly increased numbers of IFN-γ- and IL-4-producing cells than HA1-2 alone, suggesting a mixed Th1/Th2-type cellular immune response with a Th2 bias. Meanwhile, the HA1-2-fliC induced higher IgG2a and IgG1 levels, which is indicative of a mixed Th1/Th2-type profile. Consistent with this, significant levels, and equal numbers, of IFN-γ- and IL-4-producing cells were detected after HA1-2-fliC vaccination. Moreover, the marked increase in CD69 expression and the proliferative index with the HA1-2-fliC and PEI adjuvanted vaccines indicated that both adjuvanted vaccine candidates effectively induced antigen-specific cellular immune responses. Taken together, our findings indicate that the two adjuvanted vaccine candidates elicit effective and HA1-2-specific humoral and cellular immune responses, offering significant promise for the development of a successful recombinant HA1-2 subunit vaccine for H7N9 influenza.

  14. Research on TCP High Availability Technology for Embedded Network Device%嵌入式网络设备TCP高可用技术研究

    Institute of Scientific and Technical Information of China (English)

    程友清; 余少华

    2013-01-01

    This paper focus on research of TCP high availability technology for embedded double controller network device or virtual cluster switch system in data communication network. It puts forward a new TCP high availability scheme, which only makes a small amount of modification of the TCP/IP protocol stack and upper layer TCP applications. The master synchronizes TCP connection information to slave only when the TCP connection was established or closed, the TCP/IP protocol stack in slave card or device uses the received TCP synchronization information to create or close the TCP socket and store the TCP connection information into TCP control block (TCPCB), then inform the TCP socket information to upper layer TCP applications to handle. When master crashes for some reason, the slave will change to master immediately, the synchronized TCP connection on the new master initializes its TCP control block information, such as send packet sequence number and receive packet sequence number, when receives the first TCP packet of the TCP socket. After that the TCP connection has been switched successfully, the upper layer TCP applications then send or receive packet as a normal TCP connection. The scheme described above has been used in practice on high-end Ethernet switch and router developed by the author's team, the test results and practical application show that the TCP high availability scheme consumed a small a-mount of communication bandwidth to synchronize the TCP connection information from master to slave and achieved microsecond level switchover of TCP connection when master card or device going down.%对数据网络中嵌入式双主控设备和虚拟集群交换系统的TCP高可用技术进行了研究.提出一种新的TCP高可用方案,该方案对TCP/IP协议栈和TCP应用软件做少量改动.主用设备只需在TCP连接建立或关闭时向备用发送TCP连接同步信息,备用设备上TCP/IP栈收到同步信息后强制创建或关闭TCP套接字并

  15. The Role of DCT in HPV16 Infection of HaCaTs.

    Science.gov (United States)

    Aksoy, Pınar; Meneses, Patricio I

    2017-01-01

    Persistent infection with high-risk human papillomavirus (HPV) genotype is a major factor leading to many human cancers. Mechanisms of HPV entry into host cells and genome trafficking towards the nucleus are incompletely understood. Dopachrome tautomerase (DCT) was identified as a cellular gene required for HPV infection in HeLa cells on a siRNA screen study. Here, we confirm that DCT knockdown significantly decreases HPV infection in the human keratinocyte HaCaT cells as was observed in HeLas. We investigated the effects of DCT knockdown and found that DCT depletion caused increased reactive oxygen species (ROS) levels, DNA damage and altered cell cycle in HaCaT cells. We observed increased viral DNA localization at the endoplasmic reticulum but an overall decrease in infection in DCT knockdown cells. This observation suggests that viral DNA might be retained in the ER due to altered cell cycle, and viral particles are incapable of further movement towards the nucleus in DCT knockdown cells.

  16. Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.

    Science.gov (United States)

    Tanodekaew, Siriporn; Channasanon, Somruethai; Uppanan, Paweena

    2014-04-01

    The merging of stereolithography (SLA) technology to the medical field certainly benefits the manufacturing of parts, especially those patient-specific for the clinical use. This technique, however, has hardly been exploited medically due to a limited number of biodegradable resins for SLA processing. To extend application of SLA in the biomedical field, photocurable oligolactide resins were developed and examined for biodegradation and biocompatibility. The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C. The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA). The less cytotoxic sample was also obtained from the resin with higher content of HA. These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.

  17. In vitro study of RRS HA injectable mesotherapy/biorevitalization product on human skin fibroblasts and its clinical utilization

    Directory of Open Access Journals (Sweden)

    Deglesne PA

    2016-02-01

    Full Text Available Pierre-Antoine Deglesne,* Rodrigo Arroyo,* Evgeniya Ranneva, Philippe Deprez Research and Development, SKIN TECH PHARMA GROUP, Castelló d'Empúries, Spain  *These authors contributed equally to this work Abstract: Mesotherapy/biorevitalization with hyaluronic acid (HA is a treatment approach currently used for skin rejuvenation. Various products with a wide range of polycomponent formulations are available on the market. Most of these formulations contain noncross-linked HA in combination with a biorevitalization cocktail, formed by various amounts of vitamins, minerals, amino acids, nucleotides, coenzymes, and antioxidants. Although ingredients are very similar among the different products, in vitro and clinical effects may vary substantially. There is a real need for better characterization of these products in terms of their action on human skin or in vitro skin models. In this study, we analyzed the effect of the RRS® (Repairs, Refills, Stimulates HA injectable medical device on human skin fibroblasts in vitro. Skin fibroblast viability and its capacity to induce the production of key extracellular matrix were evaluated in the presence of different concentrations of RRS HA injectable. Viability was evaluated through colorimetric MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, and key extracellular matrix genes, type I collagen and elastin, were quantified by quantitative polymerase chain reaction. Results demonstrated that RRS HA injectable could promote human skin fibroblast viability (+15% and increase fibroblast gene expression of type I collagen and elastin by 9.7-fold and 14-fold in vitro, respectively. These results demonstrate that mesotherapy/biorevitalization products can, at least in vitro, effectively modulate human skin fibroblasts.Keywords: mesotherapy, medical device, RRS, collagen, elastin, extracellular matrix

  18. Availability of a remote online hemodynamic monitoring system during treatment in a private dental office for medically high-risk patients

    Directory of Open Access Journals (Sweden)

    Shinya Yamazaki

    2008-09-01

    Full Text Available Shinya Yamazaki, Hiroyoshi Kawaai, Shigeo Sasaki, Kazuhiro Shimamura, Hiroshi Segawa, Takahiro SaitoSpecial Care Department in Dentistry, Ohu University Dental Hospital, Koriyama city, Fukushima prefecture, JapanAbstract: The importance of systemic management to prevent accidents is increasing in dentistry because co-morbid illnesses in an aging society and invasive surgical procedures are increasing. In this prefecture, a new medical system called the remote online hemodynamic monitoring system (ROHMs was started in 2001. Eight private dental offices participated in this trial. When dental practitioners feel the risk of a dental procedure, they can contact via ROHMs to this hospital. Then, the hemodynamic data (blood pressure, heart rate, ECG, SpO2, and RPP of the patient in the clinic can be transmitted here via the internet, and the images and the voice can be transmitted as well. The availability of this system was assessed in 66 patients (98 cases. The most frequent complications were hypertension, heart disease, and diabetes mellitus. Systemic management included monitoring during the dental procedure (71.4%, checking vital signs after an interview (15.3%, and monitoring under sedation (13.3%. There were 35.7% of all cases where an unscheduled procedure was necessary for the systemic management. Based on a questionnaire, the majority of the patients felt relieved and safe. This system creates a situation where a specialist is almost present during the procedure. This system will provide significant assistance for future medical cooperation for risk management.Keywords: online, high-risk patient, dental treatment, medical cooperation, medical accident, risk management

  19. Efficient generation of recombinant influenza A viruses employing a new approach to overcome the genetic instability of HA segments.

    Directory of Open Access Journals (Sweden)

    Ahmed Mostafa

    Full Text Available Influenza A viruses (IAVs are the most relevant and continual source of severe infectious respiratory complications in humans and different animal species, especially poultry. Therefore, an efficient vaccination that elicits protective and neutralizing antibodies against the viral hemagglutinin (HA and neuraminidase (NA is an important strategy to counter annual epidemics or occasional pandemics. With the help of plasmid-based reverse genetics technology, it is possible that IAV vaccine strains (IVVS are rapidly generated. However, the genetic instability of some cloned HA-cDNAs after transformation into competent bacteria represents a major obstacle. Herein, we report efficient cloning strategies of different genetically volatile HA segments (H5- and H9-subtypes employing either a newly constructed vector for reverse genetics (pMKPccdB or by the use of the Escherichia coli strain HB101. Both approaches represent improved and generalizable strategies to establish functional reverse genetics systems preventing genetic changes to the cloned (HA segments of IAV facilitating more efficient rescue of recombinant IAV for basic research and vaccine development.

  20. A Novel HA/β-TCP-Collagen Composite Enhanced New Bone Formation for Dental Extraction Socket Preservation in Beagle Dogs

    Directory of Open Access Journals (Sweden)

    Ko-Ning Ho

    2016-03-01

    Full Text Available Past studies in humans have demonstrated horizontal and vertical bone loss after six months following tooth extraction. Many biomaterials have been developed to preserve bone volume after tooth extraction. Type I collagen serves as an excellent delivery system for growth factors and promotes angiogenesis. Calcium phosphate ceramics have also been investigated because their mineral chemistry resembles human bone. The aim of this study was to compare the performance of a novel bioresorbable purified fibrillar collagen and hydroxyapatite/β-tricalcium phosphate (HA/β-TCP ceramic composite versus collagen alone and a bovine xenograft-collagen composite in beagles. Collagen plugs, bovine graft-collagen composite and HA/β-TCP-collagen composite were implanted into the left and right first, second and third mandibular premolars, and the fourth molar was left empty for natural healing. In total, 20 male beagle dogs were used, and quantitative and histological analyses of the extraction ridge was done. The smallest width reduction was 19.09% ± 8.81% with the HA/β-TCP-collagen composite at Week 8, accompanied by new bone formation at Weeks 4 and 8. The HA/β-TCP-collagen composite performed well, as a new osteoconductive and biomimetic composite biomaterial, for socket bone preservation after tooth extraction.

  1. Haïti 2010 : les leçons d’une catastrophe

    Directory of Open Access Journals (Sweden)

    Jean Marie Théodat

    2010-02-01

    Full Text Available Le tremblement de terre qui a ravagé la capitale haïtienne et fait plus de 200 000 morts, constitue un événement global, d’une importance majeure qui étonne une époque habituée à percevoir ce pays comme la scène où se répète une tragédie humaine écrite depuis plus de deux siècles. On ne comprendra toutes leçons du séisme que lorsque l’onde de choc provoquée dans les consciences par ce phénomène extraordinaire aura cessé ses effets émotionnels les plus vifs. L’on pourra réfléchir alors aux inf...

  2. Effects of substrate differences on water availability for Arctic lichens during the snow-free summers in the High Arctic glacier foreland

    Science.gov (United States)

    Inoue, Takeshi; Kudoh, Sakae; Uchida, Masaki; Tanabe, Yukiko; Inoue, Masakane; Kanda, Hiroshi

    2014-12-01

    We used observational and experimental analyses to investigate the photosynthetic activity and water relationships of five lichen species attached to different substrates in a glacier foreland in the High Arctic, Ny-Ålesund, Svalbard (79°N) during the snow-free season in 2009 and 2010. After the rains ceased, lichens and their attached substrates quickly dried, whereas photosynthetic activity in the lichens decreased gradually. The in situ photosynthetic activity was estimated based on the relative electron transportation rate (rETR) in four fruticose lichens: Cetrariella delisei, Flavocetraria nivalis, Cladonia arbuscula ssp. mitis, and Cladonia pleurota. The rETR approached zero around noon, although the crustose lichen Ochrolechia frigida grown on biological soil crust (BSC) could acquire water from the BSC and retain its WC to perform positive photosynthesis. The light-rETR relationship curves of the five well-watered lichens were characterized into two types: shade-adapted with photoinhibition for the fruticose lichens, and light-adapted with no photoinhibition for O. frigida. The maximum rETR was expected to occur when they could acquire water from the surrounding air or from substrates during the desiccation period. Our results suggest that different species of Arctic lichens have different water availabilities due to their substrates and/or morphological characteristics, which affect their photosynthetic active periods during the summer.

  3. Myo-inositol hexakisphosphate degradation by Bifidobacterium pseudocatenulatum ATCC 27919 improves mineral availability of high fibre rye-wheat sour bread.

    Science.gov (United States)

    García-Mantrana, Izaskun; Monedero, Vicente; Haros, Monika

    2015-07-01

    The goal of this investigation was to develop baking products using Bifidobacterium pseudocatenulatum ATCC27919, a phytase producer, as a starter in sourdough for the production of whole rye-wheat mixed bread. This Bifidobacterium strain contributed to myo-inositol hexakisphosphate (phytate) hydrolysis, resulting in breads with higher mineral availability as was predicted by the phytate/mineral molar ratios, which remained below the inhibitory threshold values for Ca and Zn intestinal absorption. The products with sourdough showed similar technological quality as their homologous without sourdough, with levels of acetic and d/l lactic acids in dough and bread baking significantly higher with the use of sourdough. The overall acceptability scores showed that breads with 25% of whole rye flour were highly accepted regardless of the inclusion of sourdough. This work emphasises that the in situ production of phytase during fermentation by GRAS/QPS microorganisms constitutes a strategy which is particularly appropriate for reducing the phytate contents in products for human consumption.

  4. Immunopotentiation of Different Adjuvants on Humoral and Cellular Immune Responses Induced by HA1-2 Subunit Vaccines of H7N9 Influenza in Mice

    Science.gov (United States)

    Song, Li; Xiong, Dan; Hu, Maozhi; Kang, Xilong; Pan, Zhiming; Jiao, Xinan

    2016-01-01

    In spring 2013, human infections with a novel avian influenza A (H7N9) virus were reported in China. The number of cases has increased with over 200 mortalities reported to date. However, there is currently no vaccine available for the H7 subtype of influenza A virus. Virus-specific cellular immune responses play a critical role in virus clearance during influenza infection. In this study, we undertook a side-by-side evaluation of two different adjuvants, Salmonella typhimurium flagellin (fliC) and polyethyleneimine (PEI), through intraperitoneal administration to assess their effects on the immunogenicity of the recombinant HA1-2 subunit vaccine of H7N9 influenza. The fusion protein HA1-2-fliC and HA1-2 combined with PEI could induce significantly higher HA1-2-specific IgG and hemagglutination inhibition titers than HA1-2 alone at 12 days post-boost, with superior HA1-2 specific IgG titers in the HA1-2-fliC group compared with the PEI adjuvanted group. The PEI adjuvanted vaccine induced higher IgG1/IgG2a ratio and significantly increased numbers of IFN-γ- and IL-4-producing cells than HA1-2 alone, suggesting a mixed Th1/Th2-type cellular immune response with a Th2 bias. Meanwhile, the HA1-2-fliC induced higher IgG2a and IgG1 levels, which is indicative of a mixed Th1/Th2-type profile. Consistent with this, significant levels, and equal numbers, of IFN-γ- and IL-4-producing cells were detected after HA1-2-fliC vaccination. Moreover, the marked increase in CD69 expression and the proliferative index with the HA1-2-fliC and PEI adjuvanted vaccines indicated that both adjuvanted vaccine candidates effectively induced antigen-specific cellular immune responses. Taken together, our findings indicate that the two adjuvanted vaccine candidates elicit effective and HA1-2-specific humoral and cellular immune responses, offering significant promise for the development of a successful recombinant HA1-2 subunit vaccine for H7N9 influenza. PMID:26930068

  5. Immunopotentiation of Different Adjuvants on Humoral and Cellular Immune Responses Induced by HA1-2 Subunit Vaccines of H7N9 Influenza in Mice.

    Science.gov (United States)

    Song, Li; Xiong, Dan; Hu, Maozhi; Kang, Xilong; Pan, Zhiming; Jiao, Xinan

    2016-01-01

    In spring 2013, human infections with a novel avian influenza A (H7N9) virus were reported in China. The number of cases has increased with over 200 mortalities reported to date. However, there is currently no vaccine available for the H7 subtype of influenza A virus. Virus-specific cellular immune responses play a critical role in virus clearance during influenza infection. In this study, we undertook a side-by-side evaluation of two different adjuvants, Salmonella typhimurium flagellin (fliC) and polyethyleneimine (PEI), through intraperitoneal administration to assess their effects on the immunogenicity of the recombinant HA1-2 subunit vaccine of H7N9 influenza. The fusion protein HA1-2-fliC and HA1-2 combined with PEI could induce significantly higher HA1-2-specific IgG and hemagglutination inhibition titers than HA1-2 alone at 12 days post-boost, with superior HA1-2 specific IgG titers in the HA1-2-fliC group compared with the PEI adjuvanted group. The PEI adjuvanted vaccine induced higher IgG1/IgG2a ratio and significantly increased numbers of IFN-γ- and IL-4-producing cells than HA1-2 alone, suggesting a mixed Th1/Th2-type cellular immune response with a Th2 bias. Meanwhile, the HA1-2-fliC induced higher IgG2a and IgG1 levels, which is indicative of a mixed Th1/Th2-type profile. Consistent with this, significant levels, and equal numbers, of IFN-γ- and IL-4-producing cells were detected after HA1-2-fliC vaccination. Moreover, the marked increase in CD69 expression and the proliferative index with the HA1-2-fliC and PEI adjuvanted vaccines indicated that both adjuvanted vaccine candidates effectively induced antigen-specific cellular immune responses. Taken together, our findings indicate that the two adjuvanted vaccine candidates elicit effective and HA1-2-specific humoral and cellular immune responses, offering significant promise for the development of a successful recombinant HA1-2 subunit vaccine for H7N9 influenza.

  6. Information and Communications Technology (ICT) Assessment Teams for First Responders in Humanitarian Assistance/Disaster Relief (HA/DR) Missions

    OpenAIRE

    Bell, Andrew T.

    2012-01-01

    Immediately following a natural disaster requiring Humanitarian Assistance/Disaster Relief (HA/DR), a myriad of organizations respond. Typically, these early responders send small assessment teams to determine critical needs, which are then paired with the resources available. The needs can range from basic subsistence (food, shelter, and water) to transportation and infrastructure, yet the paramount factor among each team is the need to communicate. To assist in this effort, an Information a...

  7. A novel porous aspirin-loaded (GO/CTS-HA)n nanocomposite films: Synthesis and multifunction for bone tissue engineering.

    Science.gov (United States)

    Ji, Mingxiang; Li, Han; Guo, Hailin; Xie, Anjian; Wang, Shaohua; Huang, Fangzhi; Li, Shikuo; Shen, Yuhua; He, Jiacai

    2016-11-20

    A novel porous graphene oxide (GO)/chitosan (CTS)-hydroxyapatite (HA) nanocomposite film was successfully prepared for the first time by combining layer-by-layer (LBL) assembly technology with biomimetic mineralization method. The LBL technology was used to control the thickness of film as well as induce the biomimetic mineralization of biocompatible HA. The obtained (GO/CTS-HA)n film provided ideal platform for the proliferation of mouse mesenchymal stem cells (mMSCs). The pore size in the film is about 300nm, and the porous architecture made the film have high aspirin loading efficiency. Also the accumulated loading dosage could be adjusted by the film thickness, and the sustained release of aspirin could ensure well anti-inflammatory effect. The above advantages may alleviate the pain of patients and give the better environment for bone regeneration. This multifunctional aspirin-loaded (GO/CTS-HA)n film provided an inspiration for the synthesis of novel porous inorganic/biomacromolecule nanocomposite films as the biocoatings applied in bone tissue engineering.

  8. A study of the kinematics and binary-induced shaping of the planetary nebula HaTr 4

    CERN Document Server

    Tyndall, A A; Lloyd, M; O'Brien, T J; Pollacco, D

    2012-01-01

    We present the first detailed spatio-kinematical analysis and modelling of the planetary nebula HaTr 4, one of few known to contain a post-common-envelope central star system. Common envelope evolution is believed to play an important role in the shaping of planetary nebulae, but the exact nature of this role is yet to be understood. High spatial- and spectral- resolution spectroscopy of the [OIII]5007 nebular line obtained with VLT-UVES are presented alongside deep narrowband Ha+[NII]6584 imagery obtained using EMMI-NTT, and together the two are used to derive the three-dimensional morphology of HaTr 4. The nebula is found to display an extended ovoid morphology with an enhanced equatorial region consistent with a toroidal waist - a feature believed to be typical amongst planetary nebulae with post-common-envelope central stars. The nebular symmetry axis is found to lie perpendicular to the orbital plane of the central binary, concordant with the idea that the formation and evolution of HaTr 4 has been stron...

  9. An interpenetrating HA/G/CS biomimic hydrogel via Diels-Alder click chemistry for cartilage tissue engineering.

    Science.gov (United States)

    Yu, Feng; Cao, Xiaodong; Zeng, Lei; Zhang, Qing; Chen, Xiaofeng

    2013-08-14

    In order to mimic the natural cartilage extracellular matrix, a novel biological degradable interpenetrating network hydrogel was synthesized from the gelatin (G), hyaluronic acid (HA) and chondroitin sulfate (CS) by Diels-Alder "click" chemistry. HA was modified with furylamine and G was modified with furancarboxylic acid respectively. (1)H NMR spectra and elemental analysis showed that the substitution degrees of HA-furan and G-furan were 71.5% and 44.5%. Then the hydrogels were finally synthesized by cross-linking furan-modified HA and G derivatives with dimaleimide poly(ethylene glycol) (MAL-PEG-MAL). The mechanical and degradation properties of the hydrogels could be tuned simply through varying the molar ratio between furan and maleimide. Rheological, mechanical and degradation studies demonstrated that the Diels-Alder "click" chemistry is an efficient method for preparing high performance biological interpenetrating hydrogels. This biomimic hydrogel with improved mechanical properties could have great potential applications in cartilage tissue engineering. Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. Preliminary Study on c-Ha-ras Oncogene Mutations in Hydatidiform Mole Tissues

    Institute of Scientific and Technical Information of China (English)

    王芳; 谭运年; 陈碧; 李英勇; 康旭

    2001-01-01

    Objective To study the presence of c-Ha-fas oncogene mutations in hydatidiform mole (HM) tissues and to further explore its relationship with mole's malignancy Materials & methods c-Ha-ras codon 12 mutation was detected in invasive and noninvasive HM by using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Results c-Ha-fas codon 12 mutation was detected in 7 samples (53. 85%) of 13 invasive HM and 8 samples (26. 67%) in 30 non-invasive HM. c-Ha-ras mutations also showed loss of wild-type c-Ha-fas. No mutation in control group was observed.Conclusion The tendency of c-Ha-ras codon 12 mutation may be related with a higher invasive degree of HM.

  11. The Technology and Properties of Digital Double Pulse Electrodepositing Ni-HA Composite Coating of Bioceramics

    Institute of Scientific and Technical Information of China (English)

    DONG He-yan; WANG Zhou; SHI Gu-guizhi; FU Chuan-qi; CHEN Wei-rong; JIN Zhong-hong; LI Yan

    2004-01-01

    This article discusses and analyses the technology, the surface image, microstructure and ability of digital double pulse electrodepositing Ni-HA composite coatings of bioceramics made on 1Crl8Ni9Ti substrate by SEM ,XRD and so on. The results shows that ( 1 ) the HA particles exit in substrate uniformly; (2) XRD result shows that there are HA peaks at 78. 023 ° ,43. 246°and 73. 120°differently; (3) The microhardnees of the composite coatings is increased with the rise of content of HA particles, and on the same conditions the microhardnees value is greater than that of common non-pulse electrodepositing Ni-HA composite coatings of bioceramics. (4) The grain size of digital double pulse electrodepositing Ni-HA composite coatings of bioceramics is much thinner than that of common D. C.

  12. THE EFFECT OF KARANJA OIL METHYL ESTER ON KIRLOSKAR HA394DI DIESEL ENGINE PERFORMANCE AND EXHAUST EMISSIONS

    Directory of Open Access Journals (Sweden)

    Sharanappa K Godiganur

    2010-01-01

    Full Text Available Biofuels are being investigated as potential substitutes for current high pollutant fuels obtained from the conventional sources. The primary problem associated with using straight vegetable oil as fuel in a compression ignition engine is caused by viscosity. The process of transesterifiction of vegetable oil with methyl alcohol provides a significant reduction in viscosity, thereby enhancing the physical properties of vegetable oil. The Kirloskar HA394 compression ignition, multi cylinder diesel engine does not require any modification to replace diesel by karanja methyl ester. Biodiesel can be used in its pure form or can be blended with diesel to form different blends. The purpose of this research was to evaluate the potential of karanja oil methyl ester and its blend with diesel from 20% to 80% by volume. Engine performance and exhaust emissions were investigated and compared with the ordinary diesel fuel in a diesel engine. The experimental results show that the engine power of the mixture is closed to the values obtained from diesel fuel and the amounts of exhaust emissions are lower than those of diesel fuel. Hence, it is seen that the blend of karanja ester and diesel fuel can be used as an alternative successfully in a diesel engine without any modification and in terms of emission parameters; it is an environmental friendly fuel

  13. Emerged HA and NA mutants of the pandemic influenza H1N1 viruses with increasing epidemiological significance in Taipei and Kaohsiung, Taiwan, 2009-10.

    Directory of Open Access Journals (Sweden)

    Chuan-Liang Kao

    Full Text Available The 2009 influenza pandemic provided an opportunity to observe dynamic changes of the hemagglutinin (HA and neuraminidase (NA of pH1N1 strains that spread in two metropolitan areas--Taipei and Kaohsiung. We observed cumulative increases of amino acid substitutions of both HA and NA that were higher in the post-peak than in the pre-peak period of the epidemic. About 14.94% and 3.44% of 174 isolates had one and two amino acids changes, respective, in the four antigenic sites. One unique adaptive mutation of HA2 (E374K was first detected three weeks before the epidemic peak. This mutation evolved through the epidemic, and finally emerged as the major circulated strain, with significantly higher frequency in the post-peak period than in the pre-peak (64.65% vs 9.28%, p<0.0001. E374K persisted until ten months post-nationwide vaccination without further antigenic changes (e.g. prior to the highest selective pressure. In public health measures, the epidemic peaked at seven weeks after oseltamivir treatment was initiated. The emerging E374K mutants spread before the first peak of school class suspension, extended their survival in high-density population areas before vaccination, dominated in the second wave of class suspension, and were fixed as herd immunity developed. The tempo-spatial spreading of E374K mutants was more concentrated during the post-peak (p = 0.000004 in seven districts with higher spatial clusters (p<0.001. This is the first study examining viral changes during the naïve phase of a pandemic of influenza through integrated virological/serological/clinical surveillance, tempo-spatial analysis, and intervention policies. The vaccination increased the percentage of E374K mutants (22.86% vs 72.34%, p<0.001 and significantly elevated the frequency of mutations in Sa antigenic site (2.36% vs 23.40%, p<0.001. Future pre-vaccination public health efforts should monitor amino acids of HA and NA of pandemic influenza viruses isolated at

  14. Effect of high-dose phytase supplementation in broilers from 22 to 42 days post-hatch given diets severely limited in available phosphorus.

    Science.gov (United States)

    Taheri, H R; Heidari, A; Shahir, M H

    2015-01-01

    1. Two trials were conducted from 22 to 42 d post-hatch to evaluate the effectiveness of high concentrations of supplemental phytase in maize-soya bean meal-based diets severely limited in available phosphorus (P). Growth performance, plasma P and tibia ash (TA) were measured. 2. Each trial used 220 21-d-old male broilers in 20 pens with 11 birds per pen. Dietary treatments included a positive control [PC, 4.3 g/kg nonphytate P (NPP)], negative control [NC, 2.3 g/kg NPP (Trial 1) or 1.4 g/kg NPP (Trial 2)] and NC plus 1000, 2000 or 4000 phytase U/kg of the diet. 3. Birds fed on the PC diet had higher average daily gain (ADG), gain to feed ratio (G:F), plasma P (Trials 1 and 2) and TA (Trial 2) than those fed on the NC. 4. In Trial 1, ADG and G:F values of the NC plus 1000, 2000 or 4000 phytase U/kg reached those of the PC. Plasma P values of the NC plus 2000 or 4000 phytase U/kg reached that of the PC. Although TA values of the NC, NC + 1000 or NC + 2000 reached that of the PC, TA of the NC + 4000 was more than that of the PC. 5. In Trial 2, ADG and G:F values of the NC plus 4000 phytase U/kg reached those of the PC; nevertheless, plasma P values of the NC diets did not come up to that of the PC. While TA values of the NC, NC + 1000 or NC + 2000 did not reach that of the PC, TA of the NC + 4000 was greater than that of the PC. 6. Results of this study showed that, in the diets with 2.3 and 1.4 g/kg NPP, respectively, 1000 and 4000 phytase U/kg can be sufficient to obtain a comparable performance in broilers to those given diets adequate in available P.

  15. Stanley Fish, Chi ha paura di Wolfgang Iser?

    Directory of Open Access Journals (Sweden)

    Laura Massari

    2011-12-01

    Full Text Available Presentiamo qui la traduzione del saggio di Stanley Fish, Why no one’safraid of Wolfgang Iser, pubblicato nel numero di Marzo 1981 della rivista Diacritics. L’articolo fu scritto in risposta all’intervista a Wolfgang Iser, pubblicata nel numero precedente della stessa rivista, ma prende spunto per lo più da una delle massime opere di Iser, ovvero L’atto di lettura. Una teoria della risposta estetica (Il Mulino, Bologna, 1987. In esso l’autore valuta, critica e confuta molti dei capisaldi della teoria letteraria iseriana, a partire dalla natura ontologica degli agenti coinvolti nell’atto di lettura, fino all’esame fenomenologico  dell’interpretazione, che Fish applica sia al testo letterario che alla percezione del mondo reale.

  16. Near Fault Observatories: multidisciplinary research infrastructures, high resolution data and scientific products available through dedicated services implemented within the EPOS-IP project

    Science.gov (United States)

    Festa, Gaetano; Chiaraluce, Lauro; Ergintav, Semih; Bernard, Pascal; Clinton, John; Marmureanu, Alexandru; Tataru, Dragos; Vogfjord, Kristin

    2017-04-01

    external platforms and services for accessing to standard data (e.g. seismic and geodetic) and on the direct access to the e-infrastructures of individual NFOs for distribution of non standard data (e.g. strain- and tilt-meters, geochemical data, electro- magneto-telluric data) and high-level data products. To define standards for formats and metadata, the TCS actively participates into the several harmonization groups across EPOS. Two main specific services are under implementation at the TCS level. FRIDGE (EU - NFO Specific Data and Products Gateway and Virtual Laboratory) is a NFO common gateway that enables the specific data and high-level data products availability also furnishing simple visualization tools. CREW (EU - Testing Centre for Early Warning and Source characterization) is a testing facility built on real-time and offline high-resolution data, whose focus is on operating and benchmarking various existing Earthquake Early Warning (EEW) methodologies. The backbone of the testing centre is the Irpinia NFO.

  17. Comparison of publically available Moho depth and crustal thickness grids with newly derived grids by 3D gravity inversion for the High Arctic region.

    Science.gov (United States)

    Lebedeva-Ivanova, Nina; Gaina, Carmen; Minakov, Alexander; Kashubin, Sergey

    2016-04-01

    We derived Moho depth and crustal thickness for the High Arctic region by 3D forward and inverse gravity modelling method in the spectral domain (Minakov et al. 2012) using lithosphere thermal gravity anomaly correction (Alvey et al., 2008); a vertical density variation for the sedimentary layer and lateral crustal variation density. Recently updated grids of bathymetry (Jakobsson et al., 2012), gravity anomaly (Gaina et al, 2011) and dynamic topography (Spasojevic & Gurnis, 2012) were used as input data for the algorithm. TeMAr sedimentary thickness grid (Petrov et al., 2013) was modified according to the most recently published seismic data, and was re-gridded and utilized as input data. Other input parameters for the algorithm were calibrated using seismic crustal scale profiles. The results are numerically compared with publically available grids of the Moho depth and crustal thickness for the High Arctic region (CRUST 1 and GEMMA global grids; the deep Arctic Ocean grids by Glebovsky et al., 2013) and seismic crustal scale profiles. The global grids provide coarser resolution of 0.5-1.0 geographic degrees and not focused on the High Arctic region. Our grids better capture all main features of the region and show smaller error in relation to the seismic crustal profiles compare to CRUST 1 and GEMMA grids. Results of 3D gravity modelling by Glebovsky et al. (2013) with separated geostructures approach show also good fit with seismic profiles; however these grids cover the deep part of the Arctic Ocean only. Alvey A, Gaina C, Kusznir NJ, Torsvik TH (2008). Integrated crustal thickness mapping and plate recon-structions for the high Arctic. Earth Planet Sci Lett 274:310-321. Gaina C, Werner SC, Saltus R, Maus S (2011). Circum-Arctic mapping project: new magnetic and gravity anomaly maps of the Arctic. Geol Soc Lond Mem 35, 39-48. Glebovsky V.Yu., Astafurova E.G., Chernykh A.A., Korneva M.A., Kaminsky V.D., Poselov V.A. (2013). Thickness of the Earth's crust in the

  18. Enhanced Mechanical Properties and Corrosion Behavior of Biodegradable Mg-Zn/HA Composite

    Science.gov (United States)

    Salleh, Emee Marina; Zuhailawati, Hussain; Ramakrishnan, Sivakumar; Dhindaw, Brij Kumar

    2017-03-01

    Magnesium (Mg) and its alloys have shown potential for use in the biomedical industry due to their excellent biological performance and biodegradability in the bioenvironment. Thus, the aim of the present study was to develop a reliable biodegradable hard tissue substituent. Biodegradable and bioactive Mg-Zinc (Zn) reinforced by hydroxyapatite (HA) composite was prepared using mechanically alloyed Mg-6.5 wt pct Zn and pure HA powders as starting materials. Various HA contents (i.e., 5, 10, 15, and 20 wt pct) were introduced in forming the Mg-Zn/HA composite. The effect of bioactive HA incorporation in biodegradable Mg-6.5 wt pct Zn alloy matrix on mechanical and biodegradation properties as well as microstructural observation was investigated. As measured by the Williamson-Hall formula, the Mg crystallite size of the sintered composites containing 5, 10, 15, and 20 wt pct HA were 36.76, 29.08, 27.93, and 27.31 nm, respectively. According to X-ray diffraction (XRD) analysis, there was no new crystalline phase formed during milling, indicating that no mechanochemical reactions between Mg-Zn alloy and HA occurred. The -1.70 V shifted significantly toward the passive position of the plain Mg-6.5 wt pct Zn alloy and Mg-Zn/10 wt pct HA composite, which were -1.50 and -1.46 V, respectively, indicating that the Mg-Zn/10 wt pct HA composite was least susceptible to corrosion in the bioenvironment.

  19. Synthesis and Characterization of PVA-HA-Silk Composite Hydrogel by Orthogonal Experiment

    Institute of Scientific and Technical Information of China (English)

    Dekun Zhang; Kai Chen; Lin Wu; Dagang Wang; Shirong Ge

    2012-01-01

    PVA-HA-Silk composite hydrogel was synthesized with polyvinyl alcohol (PVA),nano-hydroxyapatite (HA) and natural silk by using the method of repeated freezing and thawing.A series of tests were performed to study water content,stress relaxation behavior,elastic modulus,and creep characteristics of PVA-HA-Silk composite hydrogel.Orthogonal experimental design method was used to analyze the influence degree of PVA,HA and silk (three kinds of raw materials) on mechanical properties and water content of the PVA-HA-Silk composite hydrogel to select the best material ratio according to their overall performance.The results demonstrate that the mass percentage of PVA has the greatest impact on the water content,followed by HA and silk.Compression stress-strain variation of PVA-HA-Silk composite hydrogel presents a nonlinear relationship,which proves that it is a typical viscoelastic material.Comparing the mechanical properties of 16 formulas,the formula of PVA-HA-silk composite hydrogel with mass percentage of PVA 15%,HA 2.0% and silk 1.0% is the best.

  20. Enhanced Mechanical Properties and Corrosion Behavior of Biodegradable Mg-Zn/HA Composite

    Science.gov (United States)

    Salleh, Emee Marina; Zuhailawati, Hussain; Ramakrishnan, Sivakumar; Dhindaw, Brij Kumar

    2017-05-01

    Magnesium (Mg) and its alloys have shown potential for use in the biomedical industry due to their excellent biological performance and biodegradability in the bioenvironment. Thus, the aim of the present study was to develop a reliable biodegradable hard tissue substituent. Biodegradable and bioactive Mg-Zinc (Zn) reinforced by hydroxyapatite (HA) composite was prepared using mechanically alloyed Mg-6.5 wt pct Zn and pure HA powders as starting materials. Various HA contents ( i.e., 5, 10, 15, and 20 wt pct) were introduced in forming the Mg-Zn/HA composite. The effect of bioactive HA incorporation in biodegradable Mg-6.5 wt pct Zn alloy matrix on mechanical and biodegradation properties as well as microstructural observation was investigated. As measured by the Williamson-Hall formula, the Mg crystallite size of the sintered composites containing 5, 10, 15, and 20 wt pct HA were 36.76, 29.08, 27.93, and 27.31 nm, respectively. According to X-ray diffraction (XRD) analysis, there was no new crystalline phase formed during milling, indicating that no mechanochemical reactions between Mg-Zn alloy and HA occurred. The -1.70 V shifted significantly toward the passive position of the plain Mg-6.5 wt pct Zn alloy and Mg-Zn/10 wt pct HA composite, which were -1.50 and -1.46 V, respectively, indicating that the Mg-Zn/10 wt pct HA composite was least susceptible to corrosion in the bioenvironment.

  1. Hydrophone localization for the newly installed CTBTO station HA04 using noise from the installation vessel

    Science.gov (United States)

    Nielsen, Peter; Haralabus, Georgios; Stanley, Jerry; Zampolli, Mario; Grenard, Patrick; Brown, David; Le Bras, Ronan; Amir, Menachem; Slava, Bereza; Bittner, Paulina; Gore, Jane; Wang, Haijun

    2017-04-01

    During December 2016, the cabled hydroacoustic station HA04 was installed close to the Crozet Islands, France, in the southern Indian Ocean as part of the Comprehensive Nuclear-Test-Ban Treaty Organization's (CTBTO) International Monitoring System (IMS) designed to detect nuclear explosions worldwide. The HA04 station consists of two triplets of cabled to shore hydrophones deployed north and south of Crozet. Each triplet forms a two kilometre triangular configuration positioned in the Sound Fixing and Ranging channel for optimum performance. The water depth is approximately 1200 m with the hydrophones connected to a riser cable of approximately 700 m in length. The deployment was conducted from a cable vessel utilising high precision global and dynamic positioning system to position the sensors as close as possible to the planned locations. Although high confidence in the sensor locations was achieved during the vessel operation, the locations were verified by independent means in order to refine sensor locations, optimize station performance, and to ensure that the deployment was within the tolerance of the terms of reference for the deployment. The alternative estimate of the individual sensor locations was formulated as a global optimization problem with 10 unknown parameters, i.e., latitude, longitude and depth for each sensor within a station and an effective sound speed value in water. The objective function was defined as a least-mean-square type error function between modelled and measured arrival times between pairs of hydrophones. The arrival times were obtained by cross-correlating radiated noise from the surface vessel deploying the sensors while performing a dedicated known track for the purpose of the hydrophone localization. Results from the hydrophone localization estimation are presented and compared to the planned positions together with uncertainty estimates caused by independent modelling and measurement errors.

  2. Jardines que la Comunidad de Madrid ha perdido

    Directory of Open Access Journals (Sweden)

    Carmen Ariza Muñoz

    2001-01-01

    Full Text Available Aunque se hayan perdido algunos importantes jardines, en la actualidad la Comunidad de Madrid cuenta con un mayor números de ellos. Sírvannos como ejemplos de los que ya no se conservan el primer jardín Botánico de la capital, pero en cambio existen el del paseo del Prado o los recientes de las Ciudades Universitarias de Madrid y de Alcalá de Henares. Lo mismo podría decir de los Jardines de Recreo, puesto que no nos han llegado los hechos en el siglo XIX, pero cada día surgen nuevos Parques de Atracciones y Temáticos (como el que se está realizando en San Martín de la Vega. Imnumerables son también los de las viviendas privadas o los parques públicos, que aumentan progresivamente. Sin embargo, sí han desaparecido o han cambiado de función casi todos los Reales Sitios, que surgieron, entre los siglos XVI y XIX, como consecuencia de que Madrid fuera elegida, en 1561, capital del reino por Felipe II. Ésta es la causa de que nuestra Comunidad, siendo la cuarta más pequeña de España, cuente con casi la mitad de los jardines declarados histórico-artísticos en nuestro país.

  3. A Mathematical Model of Neutral Lipid Content in terms of Initial Nitrogen Concentration and Validation in Coelastrum sp. HA-1 and Application in Chlorella sorokiniana

    Directory of Open Access Journals (Sweden)

    Zhenhua Yang

    2017-01-01

    Full Text Available Microalgae are considered to be a potential major biomass feedstock for biofuel due to their high lipid content. However, no correlation equations as a function of initial nitrogen concentration for lipid accumulation have been developed for simplicity to predict lipid production and optimize the lipid production process. In this study, a lipid accumulation model was developed with simple parameters based on the assumption protein synthesis shift to lipid synthesis by a linear function of nitrogen quota. The model predictions fitted well for the growth, lipid content, and nitrogen consumption of Coelastrum sp. HA-1 under various initial nitrogen concentrations. Then the model was applied successfully in Chlorella sorokiniana to predict the lipid content with different light intensities. The quantitative relationship between initial nitrogen concentrations and the final lipid content with sensitivity analysis of the model were also discussed. Based on the model results, the conversion efficiency from protein synthesis to lipid synthesis is higher and higher in microalgae metabolism process as nitrogen decreases; however, the carbohydrate composition content remains basically unchanged neither in HA-1 nor in C. sorokiniana.

  4. ¿Qué ha pasado con la Edad del Bronce de la Mancha?

    Directory of Open Access Journals (Sweden)

    Arturo RUIZ TABOADA

    2009-12-01

    Full Text Available RESUMEN: Este artículo asume que el estudio del origen de la complejidad social en La Mancha ha de ser analizado no sólo como un proceso que incluye la excavación y estudio de yacimientos arqueológicos sino también el entorno geográfico y la distribución y explotación de recursos. Durante las últimas décadas, el estudio del desarrollo social en la zona ha estado influido por diferentes corrientes interpretativas que van desde el Evolucionismo al Materialismo Histórico. Este artículo pretende contrastar los diferentes trabajos efectuados en la zona a la luz de los resultados de un proyecto de investigación desarrollado a principios de la década de los 90 en el sector noroeste de La Mancha.ABSTRACT: This paper argues that the emergence of social complexity in La Mancha during the Bronze Age has to be understood as a multiple process, which involves not only survey and excavation but also geographical and economic analyses and intraregional (core-periphery interaction. During the last few decades, explanations of the emergence of complex social organization in the Bronze Age of La Mancha have been related to current anthropological theories, following shifting academic fashions. These interpretations range from the first evolutionist works linking La Mancha with the South-east of Iberia at the beginning of this century, to research in the 1980s, where two main theoretical approaches, Functionalism and Historical Materialism, have converged. The aim of this paper is to discuss these interpretations and to introduce the results of a recent project that deals with the development of the settlement pattern during the Bronze Age in norwestern La Mancha. I will try to analyze how the archaeological data has been used by a number scholars to justify the aplication of some theoretical approaches.

  5. Immunological and viral determinants of dengue severity in hospitalized adults in Ha Noi, Viet Nam.

    Directory of Open Access Journals (Sweden)

    Annette Fox

    Full Text Available BACKGROUND: The relationships between the infecting dengue serotype, primary and secondary infection, viremia and dengue severity remain unclear. This cross-sectional study examined these interactions in adult patients hospitalized with dengue in Ha Noi. METHODS AND FINDINGS: 158 patients were enrolled between September 16 and November 11, 2008. Quantitative RT-PCR, serology and NS1 detection were used to confirm dengue infection, determine the serotype and plasma viral RNA concentration, and categorize infections as primary or secondary. 130 (82% were laboratory confirmed. Serology was consistent with primary and secondary infection in 34% and 61%, respectively. The infecting serotype was DENV-1 in 42 (32%, DENV-2 in 39 (30% and unknown in 49 (38%. Secondary infection was more common in DENV-2 infections (79% compared to DENV-1 (36%, p<0.001. The proportion that developed dengue haemorrhagic fever (DHF was 32% for secondary infection compared to 18% for primary infection (p = 0.14, and 26% for DENV-1 compared to 28% for DENV-2. The time until NS1 and plasma viral RNA were undetectable was shorter for DENV-2 compared to DENV-1 (p≤0.001 and plasma viral RNA concentration on day 5 was higher for DENV-1 (p = 0.03. Plasma viral RNA concentration was higher in secondary infection on day 5 of illness (p = 0.046. We didn't find an association between plasma viral RNA concentration and clinical severity. CONCLUSION: Dengue is emerging as a major public health problem in Ha Noi. DENV-1 and DENV-2 were the prevalent serotypes with similar numbers and clinical presentation. Secondary infection may be more common amongst DENV-2 than DENV-1 infections because DENV-2 infections resulted in lower plasma viral RNA concentrations and viral RNA concentrations were higher in secondary infection. The drivers of dengue emergence in northern Viet Nam need to be elucidated and public health measures instituted.

  6. Preparation of artificial canine femoral stem with HA-Ti ladder-type coating on plasma-sprayed pure Ti substrate and its performance evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Zeng Xianlin, E-mail: zxlxhh@163.com [Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 (China); Li Jingfeng; Yang Shuhua; Zheng Qixin; Zou Zhenwei [Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 (China)

    2012-03-01

    An ideal, biological-type, artificial femoral stem prosthesis has good stability and improved bone-prosthesis bonding capacity. In the current study, pure hydroxyapatite (HA)-coated, cementless, artificial femoral stems were prepared by adopting different plasma spray powers and distances and were tested in terms of shear strength. The pure titanium (Ti) substrates, HA coatings, and composite Ti-HA ladder-type coatings prepared under vacuum and atmospheric conditions were examined to compare the shear strengths, microscopic constitutions, and structures of the coatings. The coating was fabricated and the bond strength was improved by adopting 35 kW of spray power and an 80 mm spray distance. The comparisons show that the shear strength of the Ti coating prepared under vacuum conditions was higher than that of the coating prepared under atmospheric conditions (P < 0.05). Moreover, the pressure-shear strength of the Ti + HAG + Ti + V group coating was statistically significantly different from those of the HA + Ti + V and HA + Ti + A groups (P < 0.05). The coatings were compared using scanning electron microscopy, X-ray diffraction, and infrared spectrum analysis. The composite HA-Ti ladder-type coating group, where pure Ti substrate was sprayed onto the Ti alloy under vacuum conditions, had a successive laminate structure. In addition, the intergranular bond in the HA surface layer on the gradient coating was compact and highly crystallized. Under vacuum conditions, the plasma-sprayed layer was characterized by higher tightness, moderate porosity, higher bonding strength to HA, and higher HA crystallinity. The proposed coating can be used in new, cementless, artificial femoral stems with improved bone-prosthesis bonding capacity and stability.

  7. Current state of biomedical engineering; Biomedical Engineering ha ima

    Energy Technology Data Exchange (ETDEWEB)

    Sakai, K. [Waseda Univ., Tokyo (Japan). School of Science and Engineering; Kanamori, T. [National Inst. of Materials and Chemical Research, Tsukuba (Japan)

    1996-11-05

    Medical science is divided into basic medical science and clinical medicine, and the technology of the medical treatment is established as their aggregate power. This concept can be compared with the presence of industrial engineering as a product of physical science and engineering. Basic medical science has come to be combined with science deeply as a result of the rise of recent molecular biology. As to clinical medicine, current highly advanced medical treatment can be said to be made up of scientific technology. Medical treatment can be considered to include prevention, diagnosis, remedy, and rehabilitation stages. It is closely connected with engineering in each stage. The methods of approaching medical science are elucidation of the functions of internal organs and tissue considering that a living body is a plant, and offering of new therapeutical means by applying chemical devices to a living body. The functions of artificial organs can e divided roughly into convection transport, mass transfer, structural members, and signal transfer from the viewpoint of chemical engineering. Medical treatment will be brought into close relation with scientific technology in the future. 10 refs., 3 figs., 2 tabs.

  8. 76 FR 71353 - Notice of Availability of the Record of Decision for the DesertXpress Enterprises, LLC High-Speed...

    Science.gov (United States)

    2011-11-17

    ... Enterprises, LLC High-Speed Passenger Train Project AGENCY: Bureau of Land Management, Interior. ACTION... Record of Decision (ROD) for the DesertXpress Enterprises, LLC High-Speed Passenger Train Project (Desert...-managed lands to build an Electrical Multiple Unit (EMU) high-speed passenger rail line in compliance...

  9. Amino Acid Substitutions Improve the Immunogenicity of H7N7HA Protein and Protect Mice against Lethal H7N7 Viral Challenge.

    Directory of Open Access Journals (Sweden)

    Subaschandrabose Rajesh Kumar

    Full Text Available Avian influenza A H7N7/NL/219/03 virus creates a serious pandemic threat to human health because it can transmit directly from domestic poultry to humans and from human to human. Our previous vaccine study reported that mice when immunized intranasally (i.n with live Bac-HA were protected from lethal H7N7/NL/219/03 challenge, whereas incomplete protection was obtained when administered subcutaneously (s.c due to the fact that H7N7 is a poor inducer of neutralizing antibodies. Interestingly, our recent vaccine studies reported that mice when vaccinated subcutaneously with Bac-HA (H7N9 was protected against both H7N9 (A/Sh2/2013 and H7N7 virus challenge. HA1 region of both H7N7 and H7N9 viruses are differ at 15 amino acid positions. Among those, we selected three amino acid positions (T143, T198 and I211 in HA1 region of H7N7. These amino acids are located within or near the receptor binding site. Following the selection, we substituted the amino acid at these three positions with amino acids found on H7N9HA wild-type. In this study, we evaluate the impact of amino acid substitutions in the H7N7 HA-protein on the immunogenicity. We generated six mutant constructs from wild-type influenza H7N7HA cDNA by site directed mutagenesis, and individually expressed mutant HA protein on the surface of baculovirus (Bac-HAm and compared their protective efficacy of the vaccines with Bac-H7N7HA wild-type (Bac-HA by lethal H7N7 viral challenge in a mouse model. We found that mice immunized subcutaneously with Bac-HAm constructs T143A or T198A-I211V or I211V-T143A serum showed significantly higher hemagglutination inhibition and neutralization titer against H7N7 and H7N9 viruses when compared to Bac-HA vaccinated mice groups. We also observed low level of lung viral titer, negligible weight loss and complete protection against lethal H7N7 viral challenge. Our results indicated that amino acid substitution at position 143 or 211 improve immunogenicity of H7N7HA

  10. Effect of high-dose phytase and citric acid, alone or in combination, on growth performance of broilers given diets severely limited in available phosphorus.

    Science.gov (United States)

    Taheri, H R; Jabbari, Z; Adibnia, S; Shahir, M H; Hosseini, S A

    2015-01-01

    1. Two trials were conducted to evaluate the effect of high-dose phytase alone or in combination with citric acid (CA) in the diet severely limited in available phosphorus (P) on performance, plasma P and plasma Ca of broilers from 22 to 42 d of age. 2. In Trial 1, 297 21-d-old female chicks were placed into 27 pens and allocated to 9 maize-soybean meal-based dietary treatments, which were a positive control [PC, 4.23 g/kg non-phytate P (NPP)] and 8 negative control (NC, 1.35 g/kg NPP) groups consisting of two concentrations of CA (0 and 20 g/kg) and 4 concentrations of phytase (0, 1000, 2000 and 4000 U/kg) in a 2 × 4 factorial arrangement. In Trial 2, 192 21-d-old male chicks were placed into 24 pens and allocated to 6 wheat-canola meal-based dietary treatments, which were a PC (4.2 g/kg NPP), a NC (1.68 g/kg NPP) and 4 NC groups consisting of two concentrations of CA (0 and 20 g/kg) and two concentrations of phytase (2000 and 4000 U/kg) in a 2 × 2 factorial arrangement. 3. In both trials, birds fed on the PC had significantly higher average daily gain (ADG), average daily feed intake (ADFI), plasma P and lower feed conversion ratio (FCR) and plasma Ca than those of birds fed on the NC. CA supplementation significantly increased ADG and ADFI. There was a significant interaction between CA and phytase on plasma P where CA improved the effect of phytase on plasma P. In Trial 1, phytase addition improved ADG, ADFI, FCR and plasma Ca linearly. 4. Briefly, this research showed the interaction effect between CA and phytase on plasma P when broilers were fed on diets based on maize-soybean meal or wheat-canola meal. The results showed that CA supplementation lowered the concentration of phytase that is needed in low NPP diets to increase plasma P.

  11. After the Dance d’Edwidge Danticat : visions carnavalesques de l’espace haïtien

    Directory of Open Access Journals (Sweden)

    Corinne Duboin

    2008-05-01

    Full Text Available Je me propose ici d’examiner comment, dans son récit de voyage en Haïti, l’écrivaine haïtienne-américaine Edwidge Danticat perçoit le carnavalesque et l’intègre dans son discours afin de dépeindre l’espace géographique, historique et culturel de son pays d’origine et de porter par ailleurs un regard nouveau et éclairant sur soi.In my analysis of Edwidge Danticat’s travelogue, I will explore the Haitian American writer’s treatment of the carnivalesque in both her representation of her native land (its geography, history and culture and her apprehension of a new self-image.

  12. A novel PHBV/HA microsphere releasing system loaded with alendronate

    Energy Technology Data Exchange (ETDEWEB)

    Huang Wei [School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641 (China); Key Laboratory of Special Functional Materials, South China University of Technology, Ministry of Education, Guangzhou 510641 (China); Wang Yingjun, E-mail: imwangyj@scut.edu.cn [School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641 (China); Key Laboratory of Special Functional Materials, South China University of Technology, Ministry of Education, Guangzhou 510641 (China); Ren Li; Du Chang; Shi Xuetao [School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641 (China); Key Laboratory of Special Functional Materials, South China University of Technology, Ministry of Education, Guangzhou 510641 (China)

    2009-08-31

    Microspheres of poly({beta}-hydroxybutyrate-co-{beta}-hydroxyvalerate) (PHBV) incorporated with hydroxyapatite (HA) and loaded with alendronate (AL), an osteoporosis preventing drugs, were prepared by single emulsion technique. Several methods were used to evaluate this novel drug carrier microsphere system (referred as PHBV/HA-AL). Fourier transform infrared (FTIR) was used to evaluate the enwrapping of HA and the X-ray diffraction (XRD) analysis further confirmed the success. The morphology of PHBV/HA-AL microspheres was observed by scanning electron microscope (SEM), showing rough surface with HA particles enwrapped in the PHBV matrix. The in vitro drug releasing profile of PHBV/HA-AL system was investigated in a 26-day period. There is a sustained releasing pattern after a slight burst release during the first few days. Additionally, rabbit mesenchymal stem cells (MSCs) were used to evaluate the cytotoxicity of the PHBV/HA-AL composites. This controlled release system can well support the proliferation of MSCs. The novel PHBV/HA-AL controlled release system is promising for bone repair therapy.

  13. Removal of Uranium in Drinking Water: Brimac Environmental Services, Inc. Brimac HA 216 Adsorptive Media

    Science.gov (United States)

    The Brimac HA 216 Adsorptive Media was tested for uranium (U) removal from a drinking water source (well water) at Grappone Toyota located in Bow, New Hampshire. The HA 216 media is a hydroxyapatite-based material. A pilot unit, consisting of a TIGG Corporation Cansorb® C-5 ste...

  14. Effect of bioactive extruded PLA/HA composite films on focal adhesion formation of preosteoblastic cells.

    Science.gov (United States)

    Persson, Maria; Lorite, Gabriela S; Kokkonen, Hanna E; Cho, Sung-Woo; Lehenkari, Petri P; Skrifvars, Mikael; Tuukkanen, Juha

    2014-09-01

    The quality of the initial cell attachment to a biomaterial will influence any further cell function, including spreading, proliferation, differentiation and viability. Cell attachment is influenced by the material's ability to adsorb proteins, which is related to the surface chemistry and topography of the material. In this study, we incorporated hydroxyapatite (HA) particles into a poly(lactic acid) (PLA) composite and evaluated the surface structure and the effects of HA density on the initial cell attachment in vitro of murine calvarial preosteoblasts (MC3T3-EI). Scanning electron microscopy (SEM), atomic force microscopy (AFM) and infrared spectroscopy (FTIR) showed that the HA particles were successfully incorporated into the PLA matrix and located at the surface which is of importance in order to maintain the bioactive effect of the HA particles. SEM and AFM investigation revealed that the HA density (particles/area) as well as surface roughness increased with HA loading concentration (i.e. 5, 10, 15 and 20wt%), which promoted protein adsorption. Furthermore, the presence of HA on the surface enhanced cell spreading, increased the formation of actin stress fibers and significantly improved the expression of vinculin in MC3T3-E1 cells which is a key player in the regulation of cell adhesion. These results suggest the potential utility of PLA/HA composites as biomaterials for use as a bone substitute material and in tissue engineering applications.

  15. Printsess ja pühak : Püha Elisabeth Tallinna altaritel / Kaie Hiiesalu

    Index Scriptorium Estoniae

    Hiiesalu, Kaie

    2006-01-01

    Artikkel keskendub naispühakule Tüüringi Pühale Elisabethile (1207-1231). Vaatluse all on Püha Elisabethi kujutised - nikerdatud ja maalitud üksikfiguurid ning maalitud stseenid. Pikemalt peatutakse Püha Vaimu kiriku peaaltaril, kus Elisabethi on kujutatud altari kõigis kolmes asendis

  16. Registration of an oilseed sunflower germplasm HA-DM1 resistant to sunflower downy mildew

    Science.gov (United States)

    HA-DM1 (Reg. No.xxx, PI 674793) sunflower (Helianthus annuus L.) germplasm was developed and released cooperatively by the USDA-ARS, Sunflower and Plant Biology Research Unit and the North Dakota Agricultural Experiment Station in 2015. HA-DM1 is a BC2F4 derived oilseed maintainer line from the cros...

  17. High altitude dermatology

    Directory of Open Access Journals (Sweden)

    G K Singh

    2017-01-01

    Full Text Available Approximately, 140 million people worldwide live permanently at high altitudes (HAs and approximately another 40 million people travel to HA area (HAA every year for reasons of occupation, sports or recreation. In India, whole of Ladakh region, part of Northwest Kashmir, Northern part of Sikkim and Tenga valley of Arunachal are considered inhabited areas of HAA. The low quantity of oxygen, high exposure of ultraviolet (UV light, very low humidity, extreme subzero temperature in winter, high wind velocity, make this region difficult for lowlanders as well as for tourists. Acute mountain sickness, HA pulmonary edema, HA cerebral edema, and thromboembolic conditions are known to occur in HA. However, enough knowledge has not been shared on dermatoses peculiar to this region. Xerosis, UV-related skin disorders (tanning, photomelanosis, acute and chronic sunburn, polymorphic light eruption, chronic actinic dermatitis, actinic cheilitis, etc., cold injuries (frostbite, chilblains, acrocyanosis, erythrocyanosis, etc. nail changes (koilonychias, airborne contact dermatitis, insect bite reaction, and skin carcinoma (basal cell carcinomas, squamous cell carcinomas, and also rarely malignant melanoma are the dermatoses seen in HAAs. Early diagnosis and knowledge of HA dermatoses may prevent serious consequences of disease and improve the quality of life for the visitors as well as for native of the place.

  18. ANALYSIS OF C-HA-RAS GENE AMPLIFICATION AND MUTATION IN LARYNGEAL CARCINOMA

    Institute of Scientific and Technical Information of China (English)

    刘世喜; 林代诚; 洪邦泰; 黄光琦

    1995-01-01

    In order to study the ahered molecular events during laryngeal carcinogenesis and elucidate the role of Ha-ras oncogene amplification and mutation, we have examined their profile by polymerase chain reaction (PCR) and selective oligonucleoride hybridization. We analyzed the mutational status of codon 12 of Ha-ras in 22 laryngeal carcinomas and 10 normal tissues, and found that 7 of 22 laryngeal carcinomas con-tained a Ha-ras mutation at codon 12. The frequency of mutation was 32%. None of the normal tissues re-vealed mutation. Moreover, no amplification was found in cancers when compared to the normal. Our findings indicated that the aefivmed Ha-ras gene existed in laryngeal carcinoma, and activation of the Ha-ras gene by mutation at codon 12 might play a key role in laryngeal carcinogenesis.

  19. Application of VSP to geological investigation; P ha oyobi S ha VSP wo mochiita shinso chishitsu chosa

    Energy Technology Data Exchange (ETDEWEB)

    Kinugasa, Y. [Geological Survey of Japan, Tsukuba (Japan); Feng, S.; Sugiyama, T.; Ishikawa, K. [Chuoh Kaihatsu Corp., Tokyo (Japan)

    1997-05-27

    Discussed in this paper are the P-wave and S-wave zero-offset VSPs carried out utilizing boreholes located in Nada Ward, Kobe City, and Hokudan-cho, Hyogo Prefecture, as part of the deep layer boring survey following Hanshin Earthquake Disaster. This effort aims at the elucidation of P-wave and S-wave velocity structures, high-precision identification of data obtained by the surface reflection method, and collection of basic data for active faults investigation in the future. Among the velocity structures obtained for various layers, the S-wave velocity structures in particular agree with the stratigraphy excellently and may be utilized in seismic analyses to be conducted in the future. Reflection from geological boundaries is received with precision, providing accurate information about correlation between reflection and geological cross sections. The records will be useful in formulating plans for reflection surveys for instance of the boundary between the Osaka group and Kobe group. Generally speaking, reflection coefficients are large when the reflection is from a boundary where difference is great in elastic wave impedance (mainly difference in velocity). In the case of the boundary between the Kobe group and granite in Awaji Island, however, no strong reflection is found despite the great difference in velocity. This is attributed to the complicated, sharp inclination of the basement rock and to its weathering. 4 refs., 8 figs.

  20. Availability analysis of high voltage substations considering its main equipment; Analise de disponibilidade de subestacoes de alta tensao considerando seus principais equipamentos

    Energy Technology Data Exchange (ETDEWEB)

    Santos, R.C. [Universidade Federal do ABC (UFABC), Santo Andre, SP (Brazil)], E-mail: ricardo.santos@ufabc.edu.br; Basseto, B.A.; Cugnasca, P.S.; Almeida Junior, J.R.; Camargo Junior, J.B. [Universidade de Sao Paulo (USP), SP (Brazil). Escola Politecnica], Emails: ricardo.santos@ufabc.edu.br, joao.camargo@poli.usp.br

    2009-07-01

    This paper evaluates the availability of electric power substations, based on Monte Carlo simulation and Fault Tree Analysis, which can be used in a larger way, considering various scenarios, usually founded. The main fallouts modes considered in the availability analysis of power systems, as well as the main methods applied to reach this goal are discussed. The availability indexes of the Monte Carlos simulation are sufficient to indicate the critical points of the power system, and to make comparisons between different substations, related to the performance levels. Through these indexes it is possible to evaluate the impact of a switching action in a determined substation. For this, the substation studied indexes are compared before and after the switching action be considered to the substation in reference.